LOCUS       DAOMQG010000001        47183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_331_length_47183_cov_10.710745, whole genome shotgun sequence.
ACCESSION   DAOMQG010000001 DAOMQG010000000
VERSION     DAOMQG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 47183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47183
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_331_length_47183_cov_10.710745"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..683
                     /locus_tag="PLC06_00005"
     CDS             204..683
                     /locus_tag="PLC06_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO88863.1"
                     /translation="MADERKEAIKAKLAANREAFMATMGELSAEQWDMQAYSEGSEWR
                     VVDILRHVADSERGMTALMVQIKAGGGGVPEDFDLARWNQRVVNKLADKGPRELLAGM
                     ADSRQALLEFIDSLEETDWGKKGRHASLKIMTIEEVCHLIADHEALHLAGIREALGN"
     gene            complement(737..1363)
                     /locus_tag="PLC06_00010"
     CDS             complement(737..1363)
                     /locus_tag="PLC06_00010"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRO88864.1"
                     /translation="MILHVDEISHHDRYRLVIGAVIPRPIAWVSTMDADGRLNLAPFS
                     YFNAVCPSPMTLVFCPGVHADGRKKDSWRNIEAVPEFVINITNEETAEVMNQTSIVLP
                     AGQSEFEWAGLTPVPSETIRVPRVAEAPIAFECTLDRVVVISDQPGGGAAIFGRVRCI
                     HVNDAIYDNGRIDMAALRPIGRLAGDAYTRATDVFHMQRPAPVETSKM"
     gene            complement(1487..2644)
                     /locus_tag="PLC06_00015"
     CDS             complement(1487..2644)
                     /locus_tag="PLC06_00015"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRO88865.1"
                     /translation="MTYYYKLGQLPHKRHTQFRKDDGSLHHEEVMGIHGFAGIQSILY
                     HLRPPTAIRRLDLVPVEPIPYEEAGPLRPRHLRSTPLPAGGDAITGRVPLMGNSDVVL
                     YVARPTKPMDYWYKFAHGDDVLFIHEGTGVLESQFGLLRYRPGDYLVIPTGVVWRLLP
                     DPGEPQRMLVVESYGHVTPPKRYINNYGQFLENSPYCERDIRPPNELVTHDETGEFEL
                     HVRARGQTTCFQYDHHPLDVVGWDGHLWPFAFNIEDFEPITGRVHQPPPVHQTFDGPG
                     FVLCSFVPRLFDYHPLSIPAPYNHSNVDSDEVLYYVEGDFMSRKGIERASLTIHPNGI
                     PHGPHPGTYEGSIGKTRTEELAVMVDTFRPLRLTRHALEMEDDGYAYSWLT"
     gene            complement(2666..3784)
                     /gene="hppD"
                     /locus_tag="PLC06_00020"
     CDS             complement(2666..3784)
                     /gene="hppD"
                     /locus_tag="PLC06_00020"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRO88866.1"
                     /translation="MTHDFLPIKAIDHVELYVGNAKQAAHFYRTGFGFAQAAYSGLET
                     GNRQTASYVMVQGKIRLVLTTALGPDHPVARHAHLHGDGVAVIALSVPDAESAYHETT
                     IRGASGAIPPTTVEDECGVLRYSAIRAYGDTIIKFVDRSDYRGAFAPGFQPRNGYSPE
                     ADNGRAWHKGVGLAAIDHMVGNVELGAMNRWVEFFATTMGFSQLIHFDDQDISTEYSA
                     LMSKVMQDGSGKVKFPINEPAEGKKKSQIQEYLDYYYTPGVQHIACSTGDIIKTVTQL
                     RDNGIEFLRVPTTYYEDLESRVGKINEPVDKLAELGVLVDFDEEGYLLQIFTKPVEDR
                     PTVFFEIIERHGSRGFGKGNFKALFESIEREQELRGNL"
     gene            complement(3808..4977)
                     /locus_tag="PLC06_00025"
     CDS             complement(3808..4977)
                     /locus_tag="PLC06_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRO88867.1"
                     /translation="MQRSVLRQMLAVVSRPGILSFAGGLPAPDLFPTSDYSTALSTVL
                     STDTRALQYGPPFAPLKDHIVALMAERGVLCAPEEIFLTTGAQQALDVLTRYLLDAAG
                     QVLLEEFVYTGIRQAVAPYQPEVLSVQTDLETGIEVDAVVDHLEKGANPAYLYVIPDA
                     HNPLGVSISQEKRLRLADAAATYGLPIIEDDPYGFLSYDDRCLPPIRAYNDSHVFYVG
                     SFSKILAPALRLGWLVAPEALIPKLTVIKEAGDLESSALTQRAVVKYLDGGHLPEHVA
                     RLQDEYRLRRDTMLGAMERFFPSGITWTRPRAGMFIWVKLPEQIDAGALLETAVEREK
                     VAFIPGQAFVGSGVSARNFMRLNFSNCCLADIEEGIERLGRVLGAIVSDAMSQAN"
     gene            complement(5058..5543)
                     /locus_tag="PLC06_00030"
     CDS             complement(5058..5543)
                     /locus_tag="PLC06_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRO88868.1"
                     /translation="MTVSLDCIDRELLTALQRDARQTNAELARQVNLSATGLHKRLRR
                     LEEAGVIKGYVAQVDREAVGYDMLCFVQVTLQRHEPDAVDNFRREVQSMPEVLECHHL
                     TGEFDYLLKVIVCNRKHLEHFILNTLTPVRGMDKIRTSLVLSEIKATAAIPMDELSRQ
                     E"
     gene            5678..7285
                     /locus_tag="PLC06_00035"
     CDS             5678..7285
                     /locus_tag="PLC06_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88869.1"
                     /translation="MTHYVMICDYVVSSLFNNQFILFNERWAILGKPMRRTIVLLIAI
                     IFLPVSVACSGRVAVSPTPFPTAGPSPTPGSNGPITLTVTELMSAPGLYRDSVIQVTG
                     LLRKQPLIVCETKPNLSPAGWGLAEEGVLAPAGGFEQQVRSLLPDNLTMTVEGRWRQW
                     SGLVGCGKSATQQEVWFLEASRILSPSPLTQVTLTPSSGVAIAEITDTPENSLSQPEP
                     GGEFPTPALENTPVDPFSTPEPAASIGPTFDPIAPVPTGTLPGTLLITPTFEGTLAAI
                     ATSGTPSILATPTAPATGLAGTPQPTVTGTLPTPTRGASSGMIVDKGDFFIESFGDFA
                     ISSLAGGTIDSWVLDLFEGDSFNIYAVAPSPADIILSVLKDGQPIVNRQNSAPAGSPE
                     IINSAPITVDGIYEIQVLTVNSQSTDYVLVLSTDPETPVSIPGLIISGTPRSAVQLPE
                     FGYHYWVFVADAGDQISITLTPLAGEDPAIYLYDSTGEELVGADYGVEGEPEEIEETA
                     AVSGLYVISIEEIYSEPMRYDLVLTIE"
     gene            7370..8293
                     /locus_tag="PLC06_00040"
     CDS             7370..8293
                     /locus_tag="PLC06_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRO88870.1"
                     /translation="MTIIFIAVLSILLTGFMLWSSRRHQRLLSTIEVVTLPAITATQL
                     GNRRSIRVYLPPGYREREGERFEVLYINDGQEYSALGLHDTLARLSAAGRIRPIIAVA
                     IPTNENRLHEYGTAVAANDLGLGTLAQDYAHFLVNELMPRIDEAFRTRPGAGLLGVSL
                     GGLSAFDIAWNHPELFSVVGVLSGSFWWRAGNEEEKIDAGQRIAHSMARRTAHPPPVR
                     FFFQAGTRDEVGDRDGNGVIDAIQDTQELIVELQATGCSREQITYVEVLGGRHDFETW
                     ARVLPMFLEWAFSSVPGRQTRATSFDDHLLR"
     gene            complement(8232..8807)
                     /locus_tag="PLC06_00045"
     CDS             complement(8232..8807)
                     /locus_tag="PLC06_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88871.1"
                     /translation="MNEKPHIVRVVAGEARKLFFGRNRDFDAIPRPQVREDEIGVIFD
                     DGEFIPYSDLGYSGDIGFFRLSETDGVANEAAPYASGGRLIDLGQIIISTGAARRLTH
                     DEIRDLLERHAEGDYGEYGEFYELDVDDALLAGDLAWVPDRGLANKAHTLSGLDAIVS
                     AYIVREHRIWIITEAGDHQTTSLLFAGPARN"
     gene            9191..11959
                     /locus_tag="PLC06_00050"
     CDS             9191..11959
                     /locus_tag="PLC06_00050"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HRO88872.1"
                     /translation="MAGLTDLHQLGQSTWLNYLRNSFIRSGELADRVKLGIQGVTANA
                     QVYERAIAGSSDYDAGIHRAMAEGISARLIYQSLMVDDIQRAADVLLPIFKESNRLDG
                     FVSFEVDPATFSDPKAAVSGVRHISHLIDRNNVMVEVPATAVGAEIIQALIADGISVN
                     ATHLFSADDYERAALAYRDGLAHYIHANKVWHTAPTAVASFSLSPIDAAVDAALATIG
                     RQDLSGLTAIAMAKTLYRRYLDLFSGQEWDELVANGAHPMRPKWTRVTPSDFQHRADY
                     YVNNLIGPDTIVTFSPIALNTFRQEGNTIETLTTDIDAAREHLLKLSTLGIDLKAITG
                     KLQQDHLVASDRRFQSLVDAVFEKRDELDKGWRDSSWALGAAESDVEKGLQALCRQQT
                     VCRIWSHDYTVWKPQPTEITNRLGWLHIMDAMAQKAARLETFAKDIVAEGFTHALLLG
                     MGGSSLAPELFQKTFGRPARPANMPFPFLDLVVVDTTDADAIRAAEQAVNLAKTLVIV
                     STKSGGTVETLSAFKYFYNRIADKVGKERAGSHFIGITDPGSKITGLADRYHFRDVYL
                     NDSNIGGRYSALSYFGLVPAALVGVDIARLLERAAIMASNASLCHCDAIDDNLAARLG
                     TAMGKLALSGRDKITFISSLPLASFGDWVEQLIAESMGKEGKGILPVVSEPRAPVETY
                     GSDRIFVHLRLEGDDRDDEFVGRLAGAGHPVITLKLKDLYDIGGQFFLWEMATAIAGH
                     FLNINPFDQPNVEAAKIKARAVVSEYAEKGALPAGKFADLSADALNGFLSQARPGDYV
                     AIQAYVQPTAEAENALQALRQAILLRTGLATTAAFGPRYLHSTGQLHKGDRGNGLFIQ
                     FVSDAVEDVAIPDEAGEEKSSMTFNVLKKAQALGDAQALRDNHRRLISFETGPDAVRA
                     IQGVTTKS"
     gene            complement(12042..13940)
                     /locus_tag="PLC06_00055"
     CDS             complement(12042..13940)
                     /locus_tag="PLC06_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO88873.1"
                     /translation="MTTKEEKATLVVDELSPADKAHIARDAREARIRQLLRDDDELLG
                     KAYDSRLVKRLFTYIAPYKNSLIVAVILMTISSLAGTAGPAIIGFAIDKGIRANSFDV
                     LRFWTLVFLFTTITEWLTNRARVRIMAFVGTKIVADVRSELFRHLHRLTLNFYSNYSV
                     GRLMSRLIGDVGVLLDFVTWSITGLFRSVFNLVGIIIAMLLLNWQLALVAFAVMPLMI
                     ILTSYWRDRVRQAYRATRQRLALINGYLNESISGIRVTKSFTREERNFQHFDDLNLSF
                     FDANIQATRLSALFFPGVDFIGSLATALVVGVGGWLVLGDALTAGTLVAVVLYVERFF
                     DPIRELAQRYNTFQATMAASERLFELLDLEPDLVDAPGAIPLPQIRGEVEFKDVSFAY
                     REKGRPVLNNINLLAKPGERIALVGETGAGKSTIIRLLSRFYDVTDGSLTIDGHDVRE
                     VTSDSLRQQLGIVLQDTFLFTGTVADNIRYGRLNATDEEVLAASKAVGAHDFISRMPD
                     GYDSEVGENGSNLSVGQRQLVSFARALLADPRILILDEATSSVDTATERIIQRALDTL
                     MEGRTSFVIAHRLSTIINADQIIVLDHGRIIEQGTHQELLARRGRYFNLYTMQWAQQK
                     NKHLFSVN"
     gene            complement(13948..15753)
                     /locus_tag="PLC06_00060"
     CDS             complement(13948..15753)
                     /locus_tag="PLC06_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO88874.1"
                     /translation="MTEEQDNHPSKANSTWRLLGYLRPYGRYFGLAYIAMLLGTGLNL
                     IVPQIIAWAIDYGLSEGSGRTLILAGVVIMGIALVRGGLGFIQRYYGEWLTNRVAYDL
                     RNEFYNAVQYQPFAFHDQSHTGDLMSRATSDISESERFAGIGLMDLTATILLLVGVIV
                     AMLLESVPLALLAMIPLPILLIVTIHFGMTVEPMFKRIQEQMGILSTVMQESLTGIHV
                     VKAFAREPHELNKFDRENNEWFDRRQGLIKKWANNWPFMTFLIMMSVFLLLWFGGPKA
                     LEGDITVGALFALISYALMLNTPVQRLGFLVNLAATAGASASRVFEIIDLPREVVDRP
                     DALALANVNGDVKFEHVSFSYRDGLHVLADVDFHAEPGARIALIGPTGSGKTTITNLI
                     PRFYDATSGRVFVDGHDVRDLQLKSLRSHIGIVLQAPFLFSQTVKENIAYGRVDASME
                     DIIAAARVARAHDFILELDKGYDTRVGERGVTLSGGQKQRIAIARALLADPRILILDD
                     STSSVDTETEHLIQEALSELMKGRTTFIIAQRLLTLKHADSILVVDDGRIVERGTHEQ
                     LLAAEGLYRQIYDMQLRDQEEFVALQERMAPEAGG"
     gene            16015..16212
                     /locus_tag="PLC06_00065"
     CDS             16015..16212
                     /locus_tag="PLC06_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88875.1"
                     /translation="MLVKRLILAAISIGVGFGLTVLITMLIGTTPAEYGTIYMTFTTL
                     SLAIALGIWLDKFMGTNILPK"
     gene            16304..17194
                     /gene="folD"
                     /locus_tag="PLC06_00070"
     CDS             16304..17194
                     /gene="folD"
                     /locus_tag="PLC06_00070"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRO88876.1"
                     /translation="MTASIIDGKAIAESVREQVRQSVIQMKEEYGYSPGLATVLVGED
                     PASATYVRSKRKTCEDLGIRSIGHELPADATQEQVEQLVASLNADPTVNGILVQLPLP
                     KHLNEEAVLNTISLEKDVDGFHPVNIGRLAMKGRDPLFIPCTPYGCIVLLEESGIPIR
                     GAEAVVVGRSNIVGLPVAMLLQKRDATVTICHSRTRDLAEHIRNADIVIAAIGQPEMI
                     TGDMLKPGAAVIDVGINQKADPSTKRGYRLVGDVDFESAKEVAGAITPVPGGVGPMTI
                     AMLMKNTLRAAEIALAKQSH"
     gene            17226..18236
                     /locus_tag="PLC06_00075"
     CDS             17226..18236
                     /locus_tag="PLC06_00075"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO88877.1"
                     /translation="MGSGRERDLFWRGRRVFITGCTGFLGSWLTAALLERGAAVVGLI
                     RMREPESQLIRTGLIGKIEQVYGDLLDYELIKHTLTDKDIDTVFHLAGQTIVGMANRE
                     PAATFETNIRGTWLLLEAIRHTPSVRGVVVASSEKAYGEHNTLPYLEEYPLQGRHPYE
                     VSKSCADLIAQSFAHTYGLAIAVTRCSNLFGGGDLSWNRLIPGTIRSVLSGEQPVIRS
                     DGSFRRDYLYVADAVRGYLMLAEKLTEDGVRGEPFNFGSGCAVSALEVVRTIVALSGS
                     PALEPVILNEVKNEILDEFLSPAKAKQAIGWRPQYTLAGGLKETMAWYKTYLDTWDAP
                     AS"
     gene            18284..19117
                     /locus_tag="PLC06_00080"
     CDS             18284..19117
                     /locus_tag="PLC06_00080"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO88878.1"
                     /translation="MEEAEVILLLLEEFGSGTPLPAPLDKLRPNLETVYADLRNYQLT
                     ARALRQARPDKVIHLAAVGVSDPFLNVNTALSHNVTGTLNLLRACFEADGMDVSQLVV
                     ARTPGERTAMNVYAASKAAAWSFCRMYARTAQWPIQGAMIFQAFGPHQPPHLLIPAAL
                     QAALAGEDFPMTTGEQEKDWIYIDDIVAGFQALLRAELLPGTTVELGTGRATRVADVV
                     EELYGIVRHGGRPIIGAIPNRPGEAPRQIADAKQTAATINWRADLSLSEGLRKLVNSA
                     T"
     gene            19242..20078
                     /locus_tag="PLC06_00085"
     CDS             19242..20078
                     /locus_tag="PLC06_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HRO88879.1"
                     /translation="MRWPPFEHIFFDCDSTLTKVEGIDVLAEAMGKRQRVELLTQAAM
                     EGMIDLEEVYAKRLRTLRPTRQQVLDIRHSYKRNIVEDAAIVVSALQSLGHKVYIISG
                     GLAEPVEEFGVSLGIPREQIRAVGITYNELSGQWWEKSETDDIRYMTYTDGALTISDG
                     KAQIVRELLGTQRGRSLLIGDGISDLLAGKAVDLLVGFGGVVSRPKVKESAPVFIEST
                     SLAPLLGLAGGPAELRILSKWSMQYQSLSLKAQYLIQKGAVIFNHEHLESRFQQAFFS
                     TP"
     gene            20029..21153
                     /locus_tag="PLC06_00090"
     CDS             20029..21153
                     /locus_tag="PLC06_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRO88880.1"
                     /translation="MNTSNPDSSKHSSVRPDKHVKLFIPGPTEVRPEILDAQAQWMIG
                     HRMPECADLIGSILPKLSQVFFTNQTVLITASSGTGLWEAAVRNCINKKALTCVNGAF
                     ASRFSDVVGLNGKDMEILSVPWGQPVLPEQIVERLSTGEFDAVTVVHNETSTGATSPI
                     KEIAQAIRAMPGGEDIMILVDSVSGLSGARIEMDAWDLDVVLSSSQKAFALPPGLAFC
                     AVSDRAMAKAKTVPARGYYFDFISLAKSIAKNQTPATPAVSLLYALDLQLDNMMAEGM
                     ENRFARHLAMRDRTLEWSKSKGFEIFVTPGYESPTVTCLANNLEIDISALNKYLRGHG
                     MIISNGYGDLKGKTLRIAHMGDIQMYEMEELFAAMDGFFA"
     gene            21262..22848
                     /gene="serA"
                     /locus_tag="PLC06_00095"
     CDS             21262..22848
                     /gene="serA"
                     /locus_tag="PLC06_00095"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRO88881.1"
                     /translation="MYRILVSDKLGDAALEKLGEFNDVTVDVLLGHDQDQLLEIIPNY
                     DALIVRSGTQVTADMLAAAKRLRVVGRAGVGVDNIDVRAATMNGVVVMNTPGANSIAT
                     AELTLGLMLAAGRHIPQAHASLKAGEWRRGDFVGTQLYGKTLGVVGFGRVGKLVTERA
                     KAFGMDILVCDPAIMEETARDLGVLLVELDDLLAESDFITLHTALAPATIGLINAESI
                     ARMKDGAILINAARGKLIDEHAVADALRSGKLAAVALDVYSTEPPDDGNPLIDLTNVI
                     HTPHIGASTHEAERDVGIQIVNQVVSALRGTDFTYAVNLPFDVIGDFADIKPYLDLAE
                     TLGRLHAGLADKTVKRIEIEVHGDVVGNLVRAIGAGMLKGVLAATSNVPVNYINAPTL
                     ANEQGIVTTQAVGLNNLDYPNLIASRAIWNGGERLLAGVLFGGSEARIVQIDEYRLEA
                     KPEGLVLVMQNLDVPGVIGQVGTLLANHGVNIGEWRLGRVCPGGEAMSFINLDTLPGE
                     DTLRELANIEAVTQVRVVRL"
     gene            complement(22925..23746)
                     /gene="jag"
                     /locus_tag="PLC06_00100"
     CDS             complement(22925..23746)
                     /gene="jag"
                     /locus_tag="PLC06_00100"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRO88882.1"
                     /translation="MALSSNQEIESRGVTVDAAVNAGLERLGVGRDRVKIIVIDEGSR
                     GLLGIGSREAIVRLIVTPVEEAPKPPVTPAPVREAPKPAPPPAKPIERPQARAAQPVD
                     DEDEDDTSSSSEENLDAEQQTAIEVITELLDKMHVKGQVSGYVTEPDDLTGQRVNIIE
                     IKGDDLGVLIGPRGETLDSLQFLSRLMVAHRLHRRSNFVVDIEGFRQRREQALTRLAE
                     RMAEKARQRGEPIGLEPMSAYERRIIHMTLRNSADVYTESAGEGKQRRVRIIPKK"
     gene            complement(23753..24619)
                     /locus_tag="PLC06_00105"
     CDS             complement(23753..24619)
                     /locus_tag="PLC06_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRO88883.1"
                     /translation="MINALLFFYDLLGNNFILAIAVFTVVIRLITLPLNMRQQRSMMR
                     TQEIQPQIQAIQKKYRDNPQKMQEEFRRIGYNPADTLTGCLPTLIQFPILIGLYQAII
                     IVLGSTPQSLFELVPRVYSAINLTPLIPVSNKFFWMNLSQPDPFFVLPIVVFASMFIQ
                     QKLLTPSTKGQNQQDNPAAAMTQSMQYTMPLMFGFFSLQFPAGLSIYFIMANIIGIFQ
                     GLYMRRITDREKAERAANGGTPYVIDADDEKGSKAATKSSQSANGSGAAKSKSSSSSQ
                     TTTKRKKRSAKR"
     gene            complement(24665..25819)
                     /locus_tag="PLC06_00110"
     CDS             complement(24665..25819)
                     /locus_tag="PLC06_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRO88884.1"
                     /translation="MQQLRRPLRRPALWLLIIAVGVMFLTACSGRIANTNWAGLSTDG
                     SLVYLAFGPRVLAYDPATQTRQWIYPAEADTVQFYSAPSAEEGQVVFGDYGRAGGFLS
                     PRVTVSIYALGNANTNTPKELWINSSAATDRIVAPPLQVGDQVYVGTADNHILALNAK
                     DGSVTWDYEAGHAIWGKPSFRDGILYINSMDWSVYALHADTGELLWKTRLGGALAGQA
                     VLGDDLLYVSSFDGNVHALEIASGEVKWSAPASGWVWGAPAFADGVIYYSDIEGNIHA
                     ADAESGAQRWTQSTGNRIQTAPVVVGDTLYIASQNGGATPSGALTAYSTADGTQKWSQ
                     PTSAPLLATPVVVGEKTIVVGLQNADALLIGFDLATGQELWRYSLPESDN"
     gene            complement(25832..26041)
                     /gene="yidD"
                     /locus_tag="PLC06_00115"
     CDS             complement(25832..26041)
                     /gene="yidD"
                     /locus_tag="PLC06_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRO88885.1"
                     /translation="MKHIALLLIKLYQNTISRVLPPSCRFTPSCSVYGYQAIEKYGFF
                     KGGWMAIKRISRCHPFNPGGYDPVP"
     gene            complement(26074..26424)
                     /gene="rnpA"
                     /locus_tag="PLC06_00120"
     CDS             complement(26074..26424)
                     /gene="rnpA"
                     /locus_tag="PLC06_00120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRO88886.1"
                     /translation="MLPSQHRLRNSAEIGLVRREGQRRQHPFVALFVYSQPQEGAAAS
                     RFAIAVGRHIGKATRRNRVKRRIREILRARLDKMAAGYDCLIVARSGVVTASYSELES
                     ALIELLSRSGVLID"
     gene            complement(26543..26674)
                     /gene="rpmH"
                     /locus_tag="PLC06_00125"
                     /pseudo
     CDS             complement(26543..26674)
                     /gene="rpmH"
                     /locus_tag="PLC06_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003646185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
     gene            complement(26876..28168)
                     /gene="murA"
                     /locus_tag="PLC06_00130"
     CDS             complement(26876..28168)
                     /gene="murA"
                     /locus_tag="PLC06_00130"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRO88887.1"
                     /translation="MSKFIIEGGLPLGGEVTASGNKNAALKLLPACLLTDEPVTLHNI
                     PNIRDVENTILILRDLGVEMTDLGEGSWRIHAENVTKTELDPVLAARTRASFVFAGPM
                     LARMGRVALPIPGGDVIGGRPLDTHVQALTALGVSVSMAQRGVFEMAAPQLHGAGRLL
                     LAEASVTATENAVMAAVLAKGETIIDNAACEPHVRDLCEFLNSMGARIDGVGTNRLSI
                     SGVDKLCGTEFRIGSDFMEVGSFIGAAAVTKGEIRIRDAQPRNLGMIRLVYKRFGISW
                     LEDGEDIIVPGDQELAIQQGLGGRIPEIKPLPWPGFPPDLMSIAVVMGTQAAGSILLH
                     DWMYESRFFFVDHLTFMGARIVLCDPHRVLVQGPSKLFANPSGVTSPDIRAGMAMVLA
                     ALCARGTSTINNIQQIDRGYERIEAKLRGLGAEISRIE"
     gene            28269..29027
                     /locus_tag="PLC06_00135"
     CDS             28269..29027
                     /locus_tag="PLC06_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO88888.1"
                     /translation="MNGRNLHYDEKGDKGADFTLLFIHGAGGSLDVWAEETITLKGCR
                     SIALDLPGHGQSDPPGRRTVAHYAAIVEELIGSMALERPILVGHSMGSAIALTIAHRK
                     LVSLHGLILMGGSARMPVGAPILSGALADLEGVATFVAEHGAVHITDEWREVIRRQVM
                     ATGEMTTYGDYLACNRFDLRLHLSDINVPTLFIAGRQDSLVPLRFSESTAAAMPHARV
                     AILEDTGHFVMLERPQAFRGITMGFLHDPLVATR"
     gene            complement(29031..29657)
                     /gene="rdgB"
                     /locus_tag="PLC06_00140"
     CDS             complement(29031..29657)
                     /gene="rdgB"
                     /locus_tag="PLC06_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRO88889.1"
                     /translation="MTKVSDRKLLVATHNKGKIAEYTDILGNGRIAFLMLSDVGITED
                     VPETGSTFRENAILKATAYAGQTGLLTLADDSGLEVAALGGRPGVLTARYGGPGLSMA
                     ERYTLLLQEMTEIPDSLRAAQFCCVIALAAPDGTILGVSEGVCRGKIVWEPSGEAGFG
                     YDPIFYFPEYGLTMAQLRPAEKHRISHRGRAMEAIAPLLREVLNGRKP"
     gene            29745..30266
                     /locus_tag="PLC06_00145"
     CDS             29745..30266
                     /locus_tag="PLC06_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRO88890.1"
                     /translation="MKLSRLRFNFGFLLEADYGTIRQIELDYPSIKLSDDVTLTPLVG
                     FITATRTTEGIYIQGQLESSMSLECVRCLEEAIVPVNVTLDELFYYPPHIAPPGEQHV
                     GEDGMIDLAPLVRELSLLSVPIKVLCRPDCQGLCLECGANLNLGECGCETEEVDPRLA
                     VLQSLLKNDPANN"
     gene            30291..31562
                     /gene="obgE"
                     /locus_tag="PLC06_00150"
     CDS             30291..31562
                     /gene="obgE"
                     /locus_tag="PLC06_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRO88891.1"
                     /translation="MFYDEAKIHIRSGDGGDGMISFRREKFVRQGGPDGGDGGRGGNV
                     IFSASSHLNSLANFQRNKHYRAGNGVHGTVQRMNGASGDDLRLEAPPGTIIRNAETGE
                     ILADLIEPGQEVVILSGGRGGRGNQHFATSRHQAPRIAERGEPGEELWIKLELKLIAD
                     VGVIGVPNAGKSTLLSVVSAARPKIGDYPFTTLQPNLGVAAIDDYETIVLADIPGLIE
                     GASEGVGLGHDFLRHIERTRVLIHLLDGMAEDPLADFRMINKELAAYDAELAAKPQLV
                     VLNKIDLPDVRAREGKIRKAIMKAGYPFMSISAVTNEGVKALLYEVKRMVDATPRELP
                     TATISEPVVIRPPVEQQAFTIKREGGNTWRVRGKDIERIATMTYFEFDDSLLRFQTVL
                     EKSGITAALTEAGVKVGDTVYIGDQELEWGE"
     gene            31581..32786
                     /locus_tag="PLC06_00155"
     CDS             31581..32786
                     /locus_tag="PLC06_00155"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRO88892.1"
                     /translation="MTDSPDILLRKAILNNEEFVEAAFLGTRGNQTNPWARVTIRPVL
                     LKEQHLLQFEYFDSKQAFAKNYREAEAEEALMALVALPFKNVQLSTTEGTVRIQYSKK
                     GRPIIHHDAPRQKRVDLGLTHDRVKPGILSEDQAAPFLNAIGVTTADGHVRADQQRKF
                     RQINEFLRLIDETHEMEHFGHSPVHVVDLGCGSAALTFATYHYLNTIKGIPAAMTGVD
                     TKTYLLERHRETAVQLGWTTMTFEPSRIIDFQPAESPDIVLALHACDTATDEALAQAI
                     RWRSKLIFSAPCCHHHLQAQLSQATTPEPFRPVLRHGIMRERLGDVLTDSFRAHILRL
                     LGYRAEIMEFVAVEHTPKNLMIRAVRMDTPPPPALVNEYIALKAYWGVTPYLEELLRD
                     QLAPILAAA"
     gene            32821..34422
                     /locus_tag="PLC06_00160"
     CDS             32821..34422
                     /locus_tag="PLC06_00160"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRO88893.1"
                     /translation="MPATLVLTNGRIYTIDPAQPQATAVAIRDGRFLAIGSDSEMKAL
                     LGPGGEVVDLGGRFVTPGLVDAHVHFQHFALSLQNVDVEGAPTRQAALERIAAFIEQH
                     PGSGWLFGRGWVQDAWPDHAFPTAADLDDIIPDRPVYLSHKSGHAAWVNSLALRIAQV
                     NETTTDPPGGQIQRDATGRPTGILFEDAMDLVARHIPRPTVDEIALAMRRAQAYCWSV
                     GLTGLHDFDGRDCFLALQQLHEADELGLRIVKNVPVYRLEHALGVGLRSGFGDDWLRI
                     GGVKIFADGALGSRTAAMIAPYEGDPGNVGIVVTDKEEMLEKARQAGAGGLSVTVHAI
                     GDRANHDVLDVYELLRKEEANRGGPRLRHRIEHVQILHPSDLNRLAELDVIASMQPVH
                     ATSDMEMADRYWGERTRYSYAWRTMWDSGALVVFGSDAPVERIDPLPGIHAAVTRRRA
                     DGFPGKDGWHPQERLTMGETLHAFTQAAAITAGQAKTQGSISPGKLADLTIFEKDLFE
                     TPADELLNVGIAGTIVGGTFRHRTW"
     gene            complement(34487..35644)
                     /locus_tag="PLC06_00165"
     CDS             complement(34487..35644)
                     /locus_tag="PLC06_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRO88894.1"
                     /translation="MAVPEQPLTLGIEEEYQIIDAETRDLRTYISELLTQDQTREHKL
                     DLKPELMQSQVEVGSRICQNISEARAEITRLRRDVLELADENGLMIAAASTHPFARWE
                     DQIITEGTRYKELLDDMQGVARQLLIFGMHVHVGFGDDPASREMLIDTMNQARYFIPH
                     LLALSTSSPFWRGQNTGLKSYRSVVFESLPRTGIPHSFISWADYKAYEQMLERVGAFG
                     KFDKRAKIWWDIRPHPVYSTLEFRISDICTSVDDCICIAALFQAICAKLLKLRRQNMS
                     WRQYRHVHITENKWRAVRYGIEGELIDFGIQASVPVPQLIGELLEFLDDVVDELDSRR
                     EVEHARNILRDGTSADKQLRVYYEHGGDGNQDEAMRAVVDHLVAETKRNVL"
     gene            complement(35778..37274)
                     /locus_tag="PLC06_00170"
     CDS             complement(35778..37274)
                     /locus_tag="PLC06_00170"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRO88895.1"
                     /translation="MDIKIENGNIVHSGADTIIVNLFEGVTAPGGATGAVDIALGGMI
                     SDLIADGDFKGKAGEVAVLYPHGAIAARRVLVVGLGKADSFNLEGVRRASAAAIKRAR
                     DLNAQHVATIVHGAGIGGLDVKAAAQATVEGALLSTYRFDAPKKREPANEIGSLTLVE
                     FDNSRIDAIAAGAKAAEAVAAGVYLARDLVNMPPNVATPTKLAAAAQAIAGAHGMGVI
                     IGDREWAGERKMGAYLAVAQGAGEPPKFVVLEHNADRTDLPTVVLVGKGITFDTGGIS
                     IKPSEGMGSMKSDMGGAAAVLGAMETVGRLDLPLRVIGITPCTENMPDANAYRPSDII
                     TASNGKTIEIISTDAEGRMVLADALVHAETYKPDAVIDLATLTGACVVALGGGVAAGI
                     FCNDDSLRDRLLAASEATYERIWPLPLYDDYRRTINSEVADMKNSGGRMGGVGTSAVF
                     LREFTNYSWAHLDIAGMALSEKAVDYSPVGGTGFGVRLLIAYLQGMAG"
     gene            37467..38570
                     /gene="ald"
                     /locus_tag="PLC06_00175"
     CDS             37467..38570
                     /gene="ald"
                     /locus_tag="PLC06_00175"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRO88896.1"
                     /translation="MIVGVPKEIKNNEYRVAMTPAGVQQLIEQGHTVMVETGAGDGSR
                     FLDEHYARIGATIVPTAADAWSAEMVVKVKEPIAVEYQYLRSDLLLFTYLHLAAEEAL
                     THAMVDSGVTGIAYETVEAPDGSLPLLTPMSEVAGRMAIQVGAHFLERSHLGRGMLLG
                     GVAGVPGANVVVIGGGVVGTNAAQIALGMEANVTIMDTNLDRLRFLEHVLHGRLTTVA
                     SNSYNIAEAVRNADLVVGGVLIKGARAPRLVTREMISTMNLGSVIVDVAVDQGGCVET
                     IHATTHSDPVYIVDGVVHYGVANMPGAVPRTSTFALSNATLPYVLRLARVGAEAAMAE
                     NHGLALGLNTYQGKVTYKAVADAFGLEYTPYQA"
     gene            38648..>40204
                     /locus_tag="PLC06_00180"
     CDS             38648..>40204
                     /locus_tag="PLC06_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88897.1"
                     /translation="MDLLAITAQLSIINGTVQDEGTVPGLLAQPAPQKAARGRERDYL
                     FIHLALTGIPEQTAELARSLVEGLRTRFFAASGSVTSALRRAVIETNEELLRFNVEKR
                     ATHEGALSCAVLHSGELYTLQTGEGLAFLGHNFGVERLPARPPQHLTPLGRSAGIDIR
                     FAFHRLQPGDMMLLCDPRLSYLTGDTLGSVLVDTEIESGFEALMDVVAGDSARVMLVE
                     FADELPSTLPLTFQHSKQPAPKPATTKAQLKPASGKAIPVREATTETTTPVVQEEPSP
                     SFDTTAVETSARRAASSSARGLSRFIAWIAAVLEQLLGRRSDEPSIHWAIPTTVALII
                     PLIVGSVVTSVYIQRDTVNQLSQIKQQMMDELATAESLRDDAAAAQGHYLTVVSLAGQ
                     AETLRIGDIEVATMRSDALDGLDRLDGVSRLTATTFYRYAAGTNLTSLTLRGVDGGIA
                     VLDQSGNRVYFHPTDETFEEMTSEEPQTIGFGGQSVGANTIKTLFDIRWLQGSTAETR
                     DSIGMLDTTGG"
     assembly_gap    40205..40304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <40305..40962
                     /locus_tag="PLC06_00185"
     CDS             <40305..40962
                     /locus_tag="PLC06_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88898.1"
                     /translation="GSTAETRDSIGMLDTTGGLFNYYPNLGDTSGITLGNSAVWVSPT
                     AMSTYLDRLYVLDTGARKIWKYYASSGYAQFEGDETIFFSDEANLEQAIDFDLYADDG
                     SLLVLYKDGQIRYYDTHSGRIVWDENTLAQNGLMTPLIAPVSVEIVGTGLKASIFVLD
                     PGSGRLLQLSRGGMALAQYRILDASGNDVLTKAADFAVTDSPMRIFVVAGDQLLVAER
                     "
     gene            complement(40971..41756)
                     /locus_tag="PLC06_00190"
     CDS             complement(40971..41756)
                     /locus_tag="PLC06_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRO88899.1"
                     /translation="MSYETILYDVSDGVATVTLNRPSRLNAFNDQMIDETTDAFKKAG
                     RDADVRCVVITGVGRGFSSGQDLADFQGRGDDVSIGEHLRHGYHRLVRQMVSLEKPVI
                     GSINGIAAGAGCGVALAADIRIAADSASFMLAFSRIGLIPDSGTNWFLPRLIGYARAY
                     EMALTADRVPAARAMEWGMVNYVVPAEQLPEITAAWARRLADGPTLAFGLTKRSLHRS
                     WDMTLDEALEYEAHLQEVAGRSKDNREGITAFVEKREPKFIGS"
     gene            complement(41782..42147)
                     /locus_tag="PLC06_00195"
     CDS             complement(41782..42147)
                     /locus_tag="PLC06_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88900.1"
                     /translation="MEPELITIIEGPTPDFMPNPQGWMQSVYEGPHETDVALCQLRTG
                     NGSDIVERCQQAWQEGRPVRLDYPDDLRLRQQVDVVALRLTQIEEGELLLLWVTMLWE
                     EEVDEGSEMDDDIDSDIPF"
     gene            complement(42325..42855)
                     /locus_tag="PLC06_00200"
     CDS             complement(42325..42855)
                     /locus_tag="PLC06_00200"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRO88901.1"
                     /translation="MGHIPRIAFVVAMDENRLIGRDNDLPWRLPEDMGWFRRQTIGKP
                     CVMGRKTYDSLPDRFRPLPGRLNIVVTRNREYGAPGAVVVHSVDDALAAAGDAEEIVV
                     IGGADLFRRLLPVADRLYLTQVHGAAEGDVYFPEFKTDDWDEVFRQEHPADERHRFAF
                     TWLILDRNRDGAHLET"
     gene            complement(42866..43660)
                     /locus_tag="PLC06_00205"
     CDS             complement(42866..43660)
                     /locus_tag="PLC06_00205"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRO88902.1"
                     /translation="MRQYLDLMRHVIQTGTTKTDRTGVGTISVFGYQMRFDLANGFPL
                     LTTKKMHLRSIIHELLWFLRGETNVRYLRENGVTIWDEWADADGELGPIYGRQWRSWG
                     TADGTTIDQIADVVEQIRARPDSRRLIVSAWNAGEIDKMALPPCHCLFQFYVADGRLS
                     CQLYQRSADIFLGVPFNIASYALLTQMMAQVAGLQPGEFIHTLGDAHLYLNHMEQALL
                     QLSREPYPLPTMQLNPAVRSIFDFAYDDFQLLDYRSHPRIVAPIAV"
     gene            43772..43854
                     /locus_tag="PLC06_00210"
     tRNA            43772..43854
                     /locus_tag="PLC06_00210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43805..43807,aa:Leu,seq:taa)
     gene            43884..45335
                     /gene="tilS"
                     /locus_tag="PLC06_00215"
     CDS             43884..45335
                     /gene="tilS"
                     /locus_tag="PLC06_00215"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRO88903.1"
                     /translation="MSLVDQVLSALRLASNSARFDLDRDQIVVGVSGGPDSLALLHTL
                     RSLIPAGRLIVAHLDHGLRPSSAAEAQLVARAAQGLRFYTERVDVAALARQNGQSLEE
                     TGRTARYALLAQVARQEGSTHIAVGHNREDQVETILIHILRGSGLAGLRGMQPAGPLP
                     GHKGLWLLRPLLSVTREEIETYCVEKHLVPIYDESNSSYSFLRNRVRGELLPLLETYN
                     PQVRPRIIEMGEVLTAEEEYLAAQAASEWSRLIIEQEETYVILQRPGWLALPLALRRR
                     LLRLAIAAIQPGIRDVGFRALESARAIAERGETGADADLPGNITISVSYNSLIVKNEA
                     ADLANKYPQLLGLDPLPLSVPGKTPPANGWRLTAEWLDPGEFDLHDIHENRDRWQAFV
                     VLSAADTLIVRPRLPGERMRPLGLNGERKLKEIMIDRKMPAHLRRRWPILATKDHAVW
                     VVGHIIDERVRVIFGEQRVVRLQCLPPDHHEAG"
     gene            45380..45453
                     /locus_tag="PLC06_00220"
     tRNA            45380..45453
                     /locus_tag="PLC06_00220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45414..45416,aa:Pro,seq:tgg)
     gene            45464..45534
                     /locus_tag="PLC06_00225"
     tRNA            45464..45534
                     /locus_tag="PLC06_00225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45496..45498,aa:Gly,seq:tcc)
     gene            45582..45653
                     /locus_tag="PLC06_00230"
     tRNA            45582..45653
                     /locus_tag="PLC06_00230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45614..45616,aa:Ile,seq:cat)
     gene            complement(45722..47143)
                     /locus_tag="PLC06_00235"
     CDS             complement(45722..47143)
                     /locus_tag="PLC06_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88904.1"
                     /translation="MSAGYQAVTNLTAAGRFHEGRDSRAFTELLLKSAPPNSAILAHW
                     HWVTPLWYLQEIEGLRSDVEIQYVFPEGESYDAAWERRVREAHAAGLDVITTWIPVTP
                     LADLPVPEPVGEAQLYPAMPRESLPDDFVGAELSLADSIHVLGYHVDQPDNLRIGEEA
                     AITVAWRPLAKFAQGTTLFVHLVGQDDVIYAQDDKLIIPAEGITLTQFRVTPRPGISP
                     GALQVYIGTSGESVTREPLTGIIFNLSDVAPYTRNVVERLLMTSLPGTLVGYDWDHTL
                     ADRSRLYLHWRTVEGYYTQVIDDAAVPQLQLPPYRGVWGVPITDWRFPRGMEGGHYVP
                     FGQGIVWTGKAFDDLTLEPGEALVLDQAFHSAKPINRDYVVSVRLIGLEADGVHWAWW
                     DLQDSIPAMGAIPTLKWVDGSFVRSPHRVTAADSAVPGQSLTCALALYDSFTNRPLPI
                     LDERLTAENPWVPHCRGTVQPAK"
BASE COUNT        10414 a        13826 c        13284 g         9559 t
ORIGIN      
        1 gctggaatgg cgcgacggcc gctgggagat cgcacaccat cgagtcgctt atgatctcgt
       61 cgccgaagcg ctcgcgttcc gcactcacca accaccgggc tgggaggaag cagtcgccga
      121 gatcgaagcc aacggctatt attatccgca aaggatttag aaatatcctg cattacctga
      181 cccaactgga ggagacctga ccgatggcag acgaacggaa agaggcgatc aaggccaagc
      241 tggccgccaa ccgcgaggca tttatggcga cgatgggtga actgtccgcc gagcaatggg
      301 acatgcaagc ctactccgag ggatcggagt ggcgcgtcgt cgatattctc cggcacgtag
      361 ctgactcgga gcggggcatg acagcgttga tggtccagat caaggccggc gggggcggcg
      421 ttccggagga tttcgatctc gctcgctgga atcagcgcgt cgtcaacaaa ctcgccgata
      481 aaggcccgcg agagctattg gccggcatgg ccgacagccg tcaggcgctc ctggagttta
      541 ttgattcact tgaggaaact gattggggca agaaaggccg ccacgccagc ctgaagatca
      601 tgactatcga agaagtatgt cacctcatcg ccgatcatga ggccctgcat ctcgccggta
      661 ttcgcgaggc actggggaat taacgagcaa cagcaaatca taaacagcag gtcagtggac
      721 aggctgacct gccgatctac attttagacg tttcgaccgg tgcggggcgc tgcatgtgaa
      781 atacgtcggt cgcgcgggtg taggcgtcac cggccagccg accgatgggc cgcaaggccg
      841 ccatatcgat tcggccgttg tcgtaaatcg cgtcgttcac gtggatgcag cgcacccgac
      901 cgaagatagc cgcgccgccg ccgggctgat cgctgataac caccacccga tccaacgtgc
      961 attcaaaggc aatgggcgct tcggccacac gcggcacacg aattgtctcg ctcggcacgg
     1021 gggtcagccc ggcccactca aactccgact gaccggcggg caaaacaatc gaagtctggt
     1081 tcatcacttc ggccgtttcc tcattggtga tgttgatgac aaactccggc accgcctcga
     1141 tattacgcca cgaatctttc ttgcggccgt cagcatggac gccgggacaa aaaacaagcg
     1201 tcatcggaga cgggcaaaca gcgttgaaat aggagaaggg ggccaggttc aatcggccgt
     1261 cggcatccat cgtgctgacc caggcgatcg gccgtggaat cacagcgcca ataaccaatc
     1321 gataacgatc atggtgcgaa atctcgtcaa cgtggagaat caaggttaat tacctcaagc
     1381 ggaagagaaa tatacggaat ttgcgcggca tcctgggcca cgaaagcaca gattataaat
     1441 aaaaagaggt ggagcgcttt ccaaaaacgc tccatccttg actggtttag gtcagccagc
     1501 tgtaggcgta gccatcatct tccatctcca gcgcatgacg tgtcagacgc agtggccgga
     1561 aggtgtcgac catcaccgcc agctcttcag tacgcgtctt gccgatggaa ccttcatacg
     1621 tgccggggtg cggaccgtgg ggaatgccgt ttgggtggat ggtcagcgac gcgcgctcga
     1681 tacccttacg tgacatgaaa tccccctcaa cgtaatagag cacctcgtcg gaatcgacgt
     1741 tggaatgatt atacggcgcg gggatcgaca acgggtgata gtcgaacagg cgcggcacga
     1801 acgaacaaag cacaaagccg gggccatcaa atgtctggtg caccggtggc ggctggtgga
     1861 cgcggccggt aattggctcg aaatcttcga tgttgaaggc gaatggccag agatgaccat
     1921 cccaaccaac cacatcgaga gggtgatggt catactggaa acaagtagtc tggccgcgtg
     1981 ccctgacgtg aagctcaaac tcgccggtct cgtcgtgggt caccagctca ttcggcggcc
     2041 gaatgtcgcg ctcacaatag ggtgagttct ccaggaattg gccatagttg ttgatatagc
     2101 gtttgggcgg cgtgacgtgg ccataggatt ccacaacgag catgcgttgc ggctcgccgg
     2161 ggtcgggcag caagcgccag acgacgccgg tcgggatcac gagatagtcg cccggtcgat
     2221 agcgcaacag gccaaattgg ctctccagaa cgcccgtgcc ctcatgaatg aacaacacat
     2281 catcgccgtg agcgaacttg taccagtagt ccatcggttt ggtcggccga gccacataaa
     2341 gcaccacgtc actgttgccc atgagaggca cgcgcccggt gatggcgtct cctccggccg
     2401 gcaagggcgt ggaacgcaga tggcgcggcc gcagcgggcc ggcttcttca taggggatcg
     2461 gttccaccgg tacaagatcc agacggcgga tggccgtggg cggccgcaaa tggtagagaa
     2521 tggactgtat ccccgcaaag ccgtgaatgc ccatcacctc ttcgtgatgc agcgagccgt
     2581 catccttgcg gaactgggta tggcgcttgt gcggaagctg tcccagctta tagtagtatg
     2641 tcattgttgc ctccaggccg attcattaca gattcccgcg taattcctgt tcgcgttcga
     2701 tggactcgaa gagggctttg aagttgccct tgccgaaccc gcgcgagccg tgacgctcga
     2761 tgatctcaaa gaataccgtc ggccgatctt ccaccggctt ggtaaaaatt tgcagcagat
     2821 acccctcttc atcaaaatcg accagtacac ccagctcggc cagcttgtca accggctcgt
     2881 tgatcttgcc cacccgactt tccagatctt cgtagtacgt cgtgggcacg cgcaggaatt
     2941 cgatgccgtt atcccgcaac tgcgtgactg tcttaataat gtcgccggtt gaacaggcaa
     3001 tatgctggac gccgggggta tagtaatagt cgaggtactc ctgaatctgc gatttctttt
     3061 tgccttcagc cggttcattg atggggaact tcaccttgcc cgatccatcc tgcatcacct
     3121 tcgacatgag cgccgagtat tcggtagaga tatcctgatc atcaaaatgg atcaactggc
     3181 tgaaccccat cgtcgtggca aagaattcca cccaacgatt catggccccc agttcaacgt
     3241 tgccgaccat gtggtcaatg gccgccagcc caaccccctt gtgccacgcg cggccgttgt
     3301 ccgcttcggg gctatatcca ttacgaggtt gaaagccggg tgcaaacgct ccacgataat
     3361 cactccgatc gacaaatttg atgatcgtat cgccataagc gcggatagct gaatagcgca
     3421 acacgccgca ctcgtcctcg acggtcgtcg gcgggatggc ccccgacgcg ccccgaatgg
     3481 tggtctcgtg ataagcggat tcggcgtcgg gcacagacag ggcaatgaca gccacgccat
     3541 caccgtgcaa atgggcgtgc cgcgccacag gatgatcagg cccaagcgca gtcgtcagca
     3601 caagccggat tttcccctga accatcacat atgaggcggt ttgacgattg ccggtttcca
     3661 ggccgctata agccgcctgt gcaaatccaa aaccggtacg atagaaatgg gccgcctgct
     3721 tggcgttgcc aacatatagc tcgacatgat cgatcgcttt aatgggtaag aaatcgtggg
     3781 tcatcgttaa cactcctctc ggtttgatta atttgcttgt gacatcgcat cgctgactat
     3841 agcccccagc actcgcccca atcgctcgat cccctcttca atatctgcca ggcagcagtt
     3901 tgaaaaatta agtcgcatga agttgcgggc tgaaacgccc gaaccgacga acgcctgccc
     3961 cggaataaag gccactttct cccgttcgac ggccgtctcc agcaaggccc cggcatcgat
     4021 ttgctccggg agcttcaccc aaatgaacat tcccgcgcga ggccttgtcc aggttatccc
     4081 ggatgggaag aaacgctcca tggcaccgag cattgtgtca cgtcgcaagc ggtattcatc
     4141 ttgcagccgc gccacatgtt cgggtaaatg gccaccgtcc agatacttga ccacagcccg
     4201 ttgagtcagg gccgaagact ccagatcgcc ggcttccttg atgaccgtca gcttgggtat
     4261 gagcgcttcc ggcgcaacca gccaccccag ccgcaatgcc ggagcgagaa tcttggagaa
     4321 cgaaccgaca tagaacacgt ggctgtcgtt gtaggcacga atcggcggca agcaacggtc
     4381 atcgtagctc agaaagccat agggatcatc ctcgatgatc ggcaaaccat aggtcgcggc
     4441 cgcatcagcc aaccgcaagc gcttttcctg gctgatgctc acaccaagcg ggttgtgggc
     4501 atcagggatg acatataagt aggccgggtt ggctcctttc tccagatgat cgacaaccgc
     4561 atcgacctcg ataccggttt ccagatctgt ctgcaccgac aacacctcag gctggtaggg
     4621 agcaaccgcc tgacgtatgc cggtatatac gaactcctcc agcaacacct gcccggcagc
     4681 atccaacaga tagcgggtca ggacatcgag cgcttgctgg gctccggtcg tcaggaagat
     4741 ctcctctggc gcacagagca caccgcgttc ggccataagc gcaacgatgt ggtcctttaa
     4801 gggggcaaac ggtgggccat actgcaacgc gcgagtatcg gtcgaaagga ctgttgatag
     4861 agccgtgcta tagtcgctcg tcggaaataa atccggcgcg ggcaggccgc cggcaaacga
     4921 aagaatgcct ggccgagaaa cgacggccaa catctggcgc agcacggatc gttgcatacc
     4981 ctgcgtccac tgggcggatc ggatatccgg catggtaaca gagcgcgttg aaacagcagt
     5041 gtccatggga attgaggtta ctcctgccgc gacaattcat ccatcgggat ggcggccgtg
     5101 gccttgatct cgctcaggac gaggctggtg cgtatcttgt ccatgcctcg cacaggggtg
     5161 agcgtgttga ggataaaatg ttccagatgc ttgcggttgc aaacaatgac cttcagaaga
     5221 taatcaaact caccagtcag atgatggcac tccagcacct caggcatcga ttgaacttcc
     5281 cggcgaaagt tatccaccgc gtccggctca tggcgttgaa gagtaacctg aacaaagcaa
     5341 agcatgtcat agcccaccgc ctcgcggtca acctgggcta cgtagccctt gatgacaccg
     5401 gcttcttcca gccgtcgcaa tcgcttgtgc agccccgtgg ccgaaagatt gacctgtcgt
     5461 gcgagttcag cgttcgtctg ccgagcatca cgttgtaagg ctgtcagtaa ctctcggtcg
     5521 atacaatcca gtgatacagt catgccatcc aagggtaatc aaaaatgaat atgcaccaag
     5581 tataaggggt taatatcaaa tcctcaacca tttatcaaga attaggtaaa tattaacccc
     5641 aatacttagc aataggtgat tattttacac ttattatatg acgcattatg tcatgatttg
     5701 cgattatgtt gtatcgtcac tctttaacaa ccaattcatt ctatttaacg aacgctgggc
     5761 tatactgggt aagcctatga gacgaacgat cgttttactt attgctatta tctttctgcc
     5821 ggtttcggtc gcttgttcgg gtcgtgtcgc ggtttcgcca acgccctttc ccactgccgg
     5881 cccaagccct acccccggca gcaatgggcc aattaccctg acggttacgg aattgatgag
     5941 cgctcccggc ctctatcgag attcagtcat ccaggtaacc ggacttctgc gcaagcaacc
     6001 gctcatcgtc tgcgaaacga agccgaatct gtcgccagcc ggatgggggc tggccgaaga
     6061 aggcgtcctg gcgccggccg gaggatttga gcaacaagtt cgatcgctct tgcccgataa
     6121 cctgaccatg accgtcgaag ggcgctggcg acagtggtcc ggcctggtcg gctgtggcaa
     6181 gagcgccact caacaagaag tatggtttct ggaggccagc cggattctct cgcccagccc
     6241 gctcacccag gtgaccttaa cgccgtcgtc gggcgttgca atcgcggaga tcaccgacac
     6301 accggaaaat tcgctttccc agccggaacc ggggggcgaa ttccccacac ctgctctcga
     6361 gaacacgccg gtcgatccct tctccacccc ggaaccagcc gcgtcgatcg gtccgacatt
     6421 cgatccaatc gctcccgtac ccacaggcac tctgccgggg acgttactaa ttacccctac
     6481 gtttgagggc acgctcgctg ccatcgcgac ctctggcacc ccgagtatat tggctacacc
     6541 gaccgcacca gccaccggat tggccggaac gccacaacca acggtaaccg gcacacttcc
     6601 aacaccgacc aggggagcat cgtcaggcat gatcgtagac aagggagact tcttcataga
     6661 gtcgttcggc gatttcgcga tttcttcatt ggcaggcgga acgatcgata gttgggtgct
     6721 cgaccttttt gagggtgatt cattcaatat ctatgccgtt gctcccagcc cggccgatat
     6781 cattctgtct gtcctcaagg atggacaacc catcgtcaat cgtcaaaata gcgcgcctgc
     6841 cggatcgccc gagatcatca acagtgcgcc aattacggtc gatgggatct acgaaatcca
     6901 ggtactaacc gttaatagcc aatcgactga ctacgtgttg gttctgtcca ccgatccgga
     6961 gacaccggtc agtattcccg gccttattat ttcaggcacc ccgcgtagtg cggttcagtt
     7021 gccggagttt ggctatcatt attgggtctt tgtggctgat gcaggtgatc agatttccat
     7081 cacgctaaca cctttagccg gtgaggaccc agccatttat ctgtacgatt cgacagggga
     7141 agaactagtg ggagccgatt atggcgttga gggcgaacct gaagagatcg aggagacggc
     7201 tgcagttagc ggactgtatg tcatcagcat tgaagagatt tacagcgagc cgatgcggta
     7261 cgatcttgtc ttgactatcg aatagcgcca aattcgcgtg cttccgggcg acctttaatg
     7321 gcctgataac ccttacgcga ggagcaccag gcaatacatc cgcataacga tgacgatcat
     7381 tttcattgca gtactgagca tcttgctgac aggcttcatg ctgtggagca gtcgcaggca
     7441 ccaacgcctg ctcagtacca tcgaggtcgt cactttaccc gctatcactg ccactcagtt
     7501 aggaaatcgg cgaagtatca gggtatacct gcctcctggt tatcgggaac gagaaggaga
     7561 aagattcgag gttctctaca tcaacgatgg ccaggagtat agcgcgctgg gactgcatga
     7621 tacgctggcc cgactttccg cagccggccg catcagaccg atcatcgccg ttgccatacc
     7681 caccaacgag aatcgcctgc atgagtatgg cacggccgtg gcggcaaacg atctgggttt
     7741 gggcacgctg gctcaagact acgcccattt tctcgtcaac gagctgatgc cacggatcga
     7801 cgaagccttc cgcaccagac ccggggccgg attgctgggc gtctcattgg gcggcctgtc
     7861 ggcctttgat attgcctgga accaccctga gttattctcg gtggtcgggg tgctgtcggg
     7921 cagcttctgg tggcgcgccg ggaatgaaga agaaaaaatt gatgccgggc agcgaattgc
     7981 ccattcaatg gcgcgacgaa ccgcccaccc cccgccggtt cgctttttct ttcaggccgg
     8041 aacgagagac gaagtcggcg accgcgacgg caatggagtt atcgacgcaa ttcaggatac
     8101 acaggagctg attgtggaac ttcaggccac aggatgcagc cgggagcaaa taacctacgt
     8161 tgaagttctc ggcggccgcc atgattttga aacatgggcg cgggtgttgc ccatgttttt
     8221 ggagtgggcc ttcagttccg tgccgggccg gcaaacaaga gcgacgtcgt ttgatgatca
     8281 cctgcttcgg tgataatcca aatacgatgc tcgcgcacaa tgtaggctga aacgatcgca
     8341 tccagaccgc tcagcgtgtg ggccttgttg gccaggccgc gatccggaac ccaggccaaa
     8401 tcgcctgcaa ggagagcatc atccacgtcc agctcgtaga actcgccata ctctccgtaa
     8461 tcaccttctg cgtgccgttc cagaagatcg cggatttcat cgtgagtgag ccgacgggct
     8521 gcaccggtgg agatgatgat ttgccccagg tcaatgaggc ggccgccgga tgcgtaaggc
     8581 gcagcctcat tggcaacccc gtccgtctcc gataagcgaa agaacccgat gtcaccgcta
     8641 tagcccagat cgctataggg aatgaactcc ccgtcgtcga agatgacgcc tatttcatcc
     8701 tcgcgaacct gtggtctggg aatagcatcg aagtcacgat tgcgaccaaa aaacagcttg
     8761 cgagcctcgc ccgctaccac gcgaacgata tggggtttct cgttcatact gccgcctctg
     8821 ttccactacg acaaggccgc ccttggggcg gatcctcagc aaaccaatac ctgtttatat
     8881 tctaatcggc agccatcatt tgtggggaaa tttctacggg gttttcacga tgcaaacagg
     8941 caatggcgag agcgacgtat tcaccaacca cgaagagaag gcaatatgac aatgatagtt
     9001 tctctctcct gcttggaatt taggctccaa cttgctattc ttaacttgga ttgatcgccg
     9061 gacacagcag cttgcgaaac ccggtgattc tctcaacttc tccaacatat tgtcgctatt
     9121 tcatacgccg gggaaccaat ctcgatcgaa taatgagatt tcgtctccgc tttcaaagac
     9181 aggagctaac atggccggcc tgacagatct ccaccaattg ggacaatcaa cctggttgaa
     9241 ctacttgcgc aacagtttca tccgatcggg cgaacttgcc gaccgggtca agctgggcat
     9301 acagggcgtc accgccaacg ctcaggttta tgaacgagcc atcgcgggca gcagcgacta
     9361 cgacgccggg attcatcgcg ccatggccga aggcatatcg gcgcgactga tctatcaatc
     9421 gctcatggtc gacgacatcc agcgtgcggc cgatgtgctc ctgccgatat tcaaggagag
     9481 caaccgtctc gatgggttcg tcagcttcga agttgatccg gctaccttca gtgatccaaa
     9541 ggccgctgtc tccggcgtgc gtcatatctc gcatctcatc gatcgcaata acgtgatggt
     9601 tgaggtaccg gccacggcgg ttggcgccga aatcatacaa gcgctgatcg ccgacgggat
     9661 ctccgtcaac gccacccacc tgttttcggc cgatgattat gagcgagcgg ctctcgccta
     9721 tagggatgga ctcgctcatt acattcacgc caacaaggta tggcacaccg caccgaccgc
     9781 tgtagcctct ttctctctca gcccgatcga tgcggccgtc gatgcagcgc tggccacgat
     9841 cggcagacag gacctgtcgg gtcttacggc catcgctatg gccaaaaccc tctaccggcg
     9901 ctatctggac ctatttagcg gtcaggaatg ggacgaactg gtggccaatg gcgctcaccc
     9961 catgcggcca aaatggaccc gcgtcacccc aagtgatttt cagcatcgtg ctgattatta
    10021 tgtaaataat cttatcgggc cggacactat tgttactttt tcgccaatcg cgctgaatac
    10081 cttccggcaa gagggaaaca ccatcgaaac attgaccaca gatatcgacg ccgcccgcga
    10141 acatctttta aagttatcga ctctcggcat cgatctcaag gctatcaccg gcaagctcca
    10201 gcaagatcat ttggtcgcgt cggatcgccg tttccagtct ctcgttgacg cggtgtttga
    10261 aaagcgggac gaactggaca aagggtggcg cgattcctca tgggcgcttg gcgcggccga
    10321 gtccgacgtt gagaagggat tacaagcgct ttgtcggcag caaacggtct gtcgcatctg
    10381 gtcccatgac tacacggtct ggaagccaca acccacagag atcaccaatc ggctcggttg
    10441 gctgcatatt atggacgcga tggcccagaa agccgcacga ctcgaaacat tcgcaaaaga
    10501 catcgtcgcg gaagggttca cccatgccct gcttctgggc atgggcggct ccagcctggc
    10561 acccgaactg ttccagaaga ctttcgggcg gccggctcgc cccgccaata tgcccttccc
    10621 gttccttgat ctagtcgtgg ttgatacgac cgacgccgac gccatccgcg cggccgaaca
    10681 ggcggtcaat ctcgccaaaa cactggtcat cgtcagcaca aaatccggcg gcacggtgga
    10741 gacgctatcc gccttcaaat atttctacaa ccggattgcc gacaaggttg ggaaagaacg
    10801 agccggcagc cacttcatcg ggatcaccga tcccggcagc aagataacgg ggctggccga
    10861 ccgctaccac ttccgggatg tatatctcaa cgacagcaac atcggcggcc gctattcggc
    10921 cctgtcctac ttcggcctgg tgccggccgc gctcgtcggc gtcgatatcg ccaggctgct
    10981 ggaacgcgcc gccatcatgg cctcaaacgc ctcgctctgc cattgcgacg ccattgacga
    11041 caacctggcg gcacgcctcg gcacggcgat gggcaagctg gcattgtccg gccgggacaa
    11101 gatcacgttt atctcatccc tgccgttggc cagtttcggc gactgggtgg agcagctaat
    11161 cgccgagagc atgggcaagg aggggaaagg tatcctgccc gtcgtgagcg aaccgcgtgc
    11221 acctgtggag acgtatggat cggatcggat attcgtacac ctgcgattgg aaggagacga
    11281 ccgggatgat gagttcgtgg ggcggctggc gggtgccggt catcccgtaa tcaccttgaa
    11341 gctgaaagac ctgtatgaca ttggcgggca attcttcttg tgggagatgg ctaccgcgat
    11401 cgcgggacac tttctgaata tcaatccgtt tgaccaaccc aacgtagaag ccgccaaaat
    11461 caaggcgcgg gcagttgtca gcgaatacgc cgagaagggc gctctgccgg ctgggaagtt
    11521 cgccgacttg agcgccgatg cgctcaacgg cttcctgtcc caggcacggc ccggcgatta
    11581 tgtggctata caagcctacg ttcagcccac agccgaggcc gagaatgcgc tgcaagcgct
    11641 gcggcaagcc attctcctgc gcaccggcct ggccacgacg gccgcgttcg gcccacgcta
    11701 tctgcattcg acaggccaat tgcataaggg cgaccggggt aatggcctgt ttattcagtt
    11761 tgtttccgac gcagttgagg atgtcgccat acccgatgag gccggtgaag agaaatcgag
    11821 catgaccttc aacgtattga agaaagctca ggcgttgggc gacgcgcagg cattgcgcga
    11881 caaccatcga cgactcatct cattcgaaac cgggccggac gcagtcagag ccattcaagg
    11941 cgtaacgacg aagtcataga ggcccccgaa taatcaggaa gggcaaaggg ggcctgagaa
    12001 caagccatcc tttgcccttt tttgatgcgt tcaacaccca atcagttcac tgagaacagg
    12061 tgcttattct tctgctgcgc ccattgcatc gtatacaagt tgaagtagcg accgcgacga
    12121 gccaggagtt cctggtgcgt gccttgttca atgattcggc catgatcaag cacgatgatc
    12181 tggtcggcgt tgatgatggt gctcaaccgg tgggcgatga cgaagctcgt gcgtccctcc
    12241 atcaacgtgt ccagcgcgcg ctggatgatt cgctcggtgg ccgtatcaac gctgctggtc
    12301 gcctcatcga ggatcaggat gcgcggatcg gccagaagcg cccgggcaaa agagaccagt
    12361 tggcgctgac ctacgctgag gttgctgccg ttctcgccca cctcggagtc gtagccgtcg
    12421 ggcatgcggc tgatgaaatc atgagcgcca accgcttttg aggcggccag aacttcttca
    12481 tcggtggcat tgaggcggcc gtagcggatg ttatcggcga ccgtgccggt gaacagaaac
    12541 gtgtcttgca agacgatccc caactgctgc cgcaagctgt cgctggtcac ctcgcgcacg
    12601 tcatggccgt cgatcgtcaa cgagccgtca gtcacatcat agaagcgcga gagcaagcga
    12661 atgatcgtgc tcttgccggc ccccgtctcc ccaacgagtg cgatgcgctc acccggcttg
    12721 gccagcaggt taatattgtt cagaacagga cgccccttct ctcgataggc aaaagagaca
    12781 tccttgaact ccacctcgcc ccgaatttgc ggcagaggaa tcgccccggg cgcgtcgact
    12841 aaatcaggtt ccagatcaag gagctcgaat aaccgctccg acgcggccat cgtcgcctgg
    12901 aacgtgttat agcgttgcgc caactcgcga atggggtcga agaagcgctc cacatagagc
    12961 accacggcga ccagggttcc agccgtcagg gcgtctccca gcaccagcca cccgcccacg
    13021 ccgactacaa gcgccgtggc aagcgagccg ataaaatcaa cgccgggaaa gaacagcgcc
    13081 gacaagcgcg tggcctggat gttggcatcg aagaacgaca ggttcagatc gtcaaaatgc
    13141 tggaagttgc gctcctcgcg cgtgaagctc ttggtcacgc gtatgccgga gatcgactca
    13201 ttcagatagc cattgatcag tgccagtcgc tgccgcgtcg cccgatatgc ctgacgcacg
    13261 cgatcgcgcc agtagctggt caggatgatc atcaacggca tgacggcaaa cgccaccaac
    13321 gccagctgcc agttgagcaa cagcatggcg atgatgatgc cgacgaggtt gaagaccgag
    13381 cggaacaggc cggtgatcga ccaggtgacg aaatcgagca agacgccgac gtcgccaata
    13441 aggcggctca tcaggcggcc gacgctgtaa ttgctgtaga agttcagcgt caggcgatgc
    13501 agatggcgga atagctcgct gcgcacatca gcgacgatct tcgtgccgac aaatgccatg
    13561 atgcgcacac gcgcgcggtt agtcagccat tcggtgatcg tcgtgaacag aaataccagc
    13621 gtccaaaatc tgagcacgtc gaacgagttt gcccgaatac ccttgtcgat agcgaagccg
    13681 ataatcgccg gcccggcagt gcctgccaga gagctgatag tcatcaggat tacggcgaca
    13741 atgaggctgt ttttgtaggg cgcgatgtag gtgaaaaggc gcttcaccag gcggctgtca
    13801 taagccttgc ccaacaactc atcatcgtcg cgcagtaatt gccgaatgcg tgcctcacgg
    13861 gcatcgcgcg cgatatgagc cttgtcggcc ggagatagtt cgtcaacgac gagcgttgct
    13921 ttttcttctt ttgttgtcat ctcgtcctca acctcccgcc tcgggggcca tccgctcttg
    13981 cagcgccaca aactcttcct ggtctctcaa ctgcatgtcg taaatctgtc gatagagacc
    14041 ttccgcagcc agcagttgct cgtgtgttcc gcgctccacg atgcggccgt catccacgac
    14101 caaaatactg tcagcgtgct taagcgtcag cagtcgctga gcgatgatga acgtcgttcg
    14161 ccccttcatc agctccgaca acgcttcctg gatgagatgc tccgtttcgg tatccacgct
    14221 gctggtggag tcgtcgagga tcaggatgcg cgggtcggcc agcaaggcgc gcgcgatggc
    14281 gatgcgttgc ttctggccgc cgctcaacgt cacgccacgc tcccccaccc gggtgtcata
    14341 ccctttatcc aattcgagaa taaagtcatg ggcacgagcc actcgagcgg cggcgatgat
    14401 gtcttccatc gacgcatcga cgcggccata agcgatattt tctttcaccg tctggctgaa
    14461 gaggaacggc gcctgcaaga caatgccgat gtgggagcga aggctcttca gctgcaaatc
    14521 acgcacgtcg tggccgtcca caaagactcg gccgctggtg gcgtcataaa agcggggaat
    14581 gaggttcgtg atggttgtct tgcccgaacc cgtggggcca attagcgcga tgcgcgcgcc
    14641 cggttcggcg tggaaatcaa catccgccag cacgtggagg ccgtcgcgat aggagaacga
    14701 cacatgctcg aacttcacgt ccccattcac gtttgccaat gccaacgcat caggacgatc
    14761 gacgacctct cgcggcagat caatgatctc gaacacacgg gatgcgcttg ccccggccgt
    14821 ggccgcaagg ttgaccagaa accccaggcg ctgtaccggc gtattgagca tgagcgcata
    14881 tgaaatcagg gcaaagagag cgccaaccgt gatatcgcct tccagagcct tggggccgcc
    14941 aaaccagagc aggagaaaga cgctcatcat gatcaggaag gtcataaacg gccagttatt
    15001 ggcccatttc ttgattagcc cctggcggcg atcgaaccac tcattatttt cgcgatcgaa
    15061 tttattcagc tcatgcggct cacgcgcaaa cgccttgacc acatgaatcc ccgtgaggct
    15121 ctcctgcatc accgtggaca gaatgcccat ctgctcctgg atgcgcttaa acattggttc
    15181 gaccgtcatg ccgaaatgaa tcgtcacaat gagcaatatc ggcagaggga tcatcgcaag
    15241 caacgccagg gggacggatt ccagcagcat cgccacgatc acaccgacca gcaacagaat
    15301 cgtcgcggtc aagtccatca gaccgatgcc ggcaaatcgc tccgattcac taatatcgct
    15361 cgtggctcgg ctcatcaagt caccggtgtg gctctggtca tggaacgcaa aaggttgata
    15421 ttgaaccgcg ttgtagaact cattgcgcag gtcataggcc acacggttgg tcaaccactc
    15481 gccgtaatag cgttggatga accccaggcc gccacgaacg agcgcgatgc ccatgatgac
    15541 tacgccggcc agaatcaacg ttcggccgct tccctcggat agcccgtaat caatcgccca
    15601 ggcgatgatt tgcggaacga taagattgag gcccgttccc aggagcatgg cgatataagc
    15661 caaaccaaaa tagcgaccat atggccgcag ataccccaga aggcgccacg tggaatttgc
    15721 cttcgatgga tgattgtctt gctcttcggt cacacgtata ccccctctgt ggagttaaat
    15781 ttgagccgat tggataaagc cggagcaata acgatcggtc agcgtggtga gtgtaaaata
    15841 cgtaacggca attataccat aaattttgcc gggcattagg tatttatcta attcccgtca
    15901 aaacctgtga cctgaaatgg tgtgaggttc aatcaataca ctggcttgcg gccacatagc
    15961 aatctgctac aattgccctg gtttgtgatg gaacatgatt ggaggaattt atccatgttg
    16021 gtaaaacgac ttattctggc agccatctcc attggtgtcg ggtttggcct gactgtattg
    16081 atcacgatgc tgatcggcac aaccccggct gagtatggca ccatctatat gaccttcacg
    16141 actttatcgt tggcgatcgc ccttggaatc tggctggaca aattcatggg caccaatatc
    16201 ctgccgaagt aggagccaat tttcacctcc cccgcggact tatttttagg cctctcattt
    16261 caggtagagg ctttttttgt ttcacgacag gaaggtaaac aaaatgacag cttcaatcat
    16321 tgacggcaag gcaattgccg agagcgtgcg cgagcaagtt cgtcaatccg tcatccaaat
    16381 gaaggaagag tatgggtact ctcccggcct ggcgacggtt ctggtgggag aagaccctgc
    16441 ctcggcaacg tatgttcgca gcaagcgcaa gacctgcgaa gatctgggaa ttcggtcgat
    16501 cggccatgag ttgccggccg atgcgacaca agagcaggtc gagcagttag tggcgtcttt
    16561 gaacgctgat ccaaccgtca acggcattct cgttcagctt ccgctcccca aacatttgaa
    16621 tgaggaagcc gtcctgaata cgatcagtct ggagaaagat gtcgatggct ttcatccggt
    16681 gaatatcggg cgcctggcca tgaaaggtcg cgatccgctg ttcatcccct gcacgcccta
    16741 cggctgcatc gttctactgg aagagagcgg catccccatc cgtggcgcgg aagcggtcgt
    16801 ggtcggccgt tccaatatcg tcggcctgcc ggtagccatg ctgctgcaga agcgcgacgc
    16861 gacggtgacg atttgccata gccgcacccg cgacctggcc gaacacatcc gcaacgccga
    16921 tatcgtcatc gcggctatcg gccaaccgga gatgatcacc ggagatatgt tgaagcccgg
    16981 cgcggccgtc atcgacgtcg gcattaacca aaaggccgat ccctcaacca aacgtggcta
    17041 ccgactggtc ggcgacgtgg attttgaatc ggccaaggaa gtggctggcg cgatcacgcc
    17101 ggtcccgggc ggtgtggggc caatgaccat cgccatgctg atgaagaaca ccctgcgggc
    17161 ggccgaaatc gccctggcca aacagagcca ttagccgcgc gcaatcatta ttggagagaa
    17221 agcaattggg ttcgggacgt gaacgggatt tgttctggcg cgggcgacgg gttttcatca
    17281 ccggttgcac cggcttcctg ggctcatggc tgacggccgc gctgctggaa cgcggcgcgg
    17341 ccgtcgttgg gctgattcgc atgcgcgaac ccgaatctca actcatcaga acagggttga
    17401 tcgggaaaat tgagcaggtg tatggcgatc ttctcgatta cgagctgatc aaacacacgc
    17461 tgaccgacaa agacatcgac accgtctttc atctggcggg gcaaacaatc gttggaatgg
    17521 ccaatcggga acccgcggcc acattcgaga cgaacatacg cggcacgtgg cttttgctgg
    17581 aggcgatccg acatacgccc agcgttcgcg gagtcgtcgt tgccagcagc gagaaggctt
    17641 acggcgaaca caacaccctg ccataccttg aggagtatcc acttcaagga aggcacccct
    17701 acgaagtatc aaagagctgc gccgacctaa tcgcccagtc gtttgcccac acttacgggc
    17761 tggcaatcgc cgtaacgcgc tgttccaatc tctttggcgg cggcgacctg agctggaatc
    17821 gcctcatccc cggcacgatc cgcagtgtgc taagtggtga gcaaccggtc attcgatccg
    17881 atggctcttt ccggcgcgac tatctctatg tggccgatgc cgtccgcggc tatctgatgc
    17941 tggccgaaaa gttgaccgag gatggggtgc ggggcgaacc cttcaatttt ggctcaggct
    18001 gtgcggtctc ggcattagag gtagtgcgca ctatcgtcgc cctgtccggc agcccggcgc
    18061 ttgagcctgt catccttaac gaagttaaaa acgaaatcct cgacgaattc ctaagcccgg
    18121 caaaagctaa acaagccatc ggctggcggc cgcaatacac gctggccgga gggctgaagg
    18181 agacaatggc ctggtacaag acctacctcg atacatggga cgcccccgca tcctaatcac
    18241 cggcgccacc ggttttatcg cgcggtctct tgtgccgaaa ctcatggagg aggcggaggt
    18301 gattcttctg cttctggaag aattcggcag cggaacgccc cttcccgcgc cactcgacaa
    18361 gctgcggcca aatctggaaa cggtctatgc cgatctgcgc aattaccagc tcacagctcg
    18421 cgcattacgg caggcccggc ccgacaaagt cattcatctg gcagctgtcg gcgtcagcga
    18481 tccctttctc aacgtcaaca cggccttgag ccacaacgtg accggcacac tgaacttgtt
    18541 gcgtgcgtgc ttcgaggcag atggcatgga tgtgtcacaa ctcgtcgttg cacgtactcc
    18601 gggcgagcgc accgcgatga acgtctatgc agccagcaaa gccgccgcgt ggtcgttctg
    18661 caggatgtac gcacgcactg ctcaatggcc catccagggg gccatgatat tccaggcgtt
    18721 cggccctcac cagccacccc atctcctgat accggccgcg cttcaggcgg ctctggccgg
    18781 ggaagatttc cccatgacca cgggagaaca ggagaaagac tggatctaca tcgacgacat
    18841 tgtcgccgga tttcaagctc tcctgagggc cgaattgtta ccggggacga cggtggaatt
    18901 gggcaccggc cgcgccaccc gcgtggctga cgtggtcgag gagctgtacg ggattgttcg
    18961 ccatggcggg cgaccaatca tcggtgccat ccccaaccgg ccgggtgagg ccccccggca
    19021 gattgccgat gccaaacaaa cggcggcaac tatcaattgg cgagccgatc tatcgctcag
    19081 cgaggggctc cggaagctgg tcaacagtgc tacataagaa tcaactgcca tgcaatcacc
    19141 cgttggcaaa atgcgttatt cttaatagaa tcgccggtta atataaggtc gctacctgtt
    19201 agatacttta ccccctgaac gaagaaccaa tctgaatcac tatgcgttgg cccccttttg
    19261 agcatatctt tttcgattgc gattccaccc tgaccaaggt ggagggtatc gacgtgcttg
    19321 ccgaagccat gggcaagcgg caacgtgttg aattgttgac acaagcggcg atggagggca
    19381 tgatcgacct ggaggaggtg tatgccaaac gactgcgtac actcaggccc acaaggcaac
    19441 aagtccttga catccgccac agttacaaac gaaatattgt tgaggatgcg gcgatcgtgg
    19501 tatccgcgct gcaatctctg ggccataagg tgtacatcat cagtggcgga ttggccgagc
    19561 cggtcgaaga gttcggcgtc tctctgggta tcccgcgcga gcaaatacgc gcggtaggca
    19621 tcacctataa tgagctgtcc ggccaatggt gggaaaagtc ggagaccgat gacattcgct
    19681 acatgaccta caccgatggc gcgctcacga ttagtgacgg aaaggcccag attgtccgcg
    19741 aactattggg aacccaacgc ggccgttctc ttttgatcgg tgacggcata agcgatctgc
    19801 tggccggaaa agcagtcgac ctgcttgtcg gctttggggg cgtcgtatcc cgccccaagg
    19861 tgaaggagag cgcgcctgtt tttatcgaga gcaccagcct ggccccgctg ttggggctgg
    19921 ccggtggccc ggccgaatta cgaatcctga gcaagtggtc gatgcaatat caatcactca
    19981 gtctgaaagc ccaatatttg attcaaaaag gagcggtgat atttaaccat gaacacctcg
    20041 aatccagatt ccagcaagca ttcttcagta cgccctgaca agcacgtcaa gctgttcatc
    20101 cccggcccaa ccgaagttcg gccggaaatc ctcgacgcgc aagcccaatg gatgatcggc
    20161 catcgcatgc cggagtgtgc cgatctcatc ggtagcatcc tgccgaagct gtcacaagtc
    20221 ttcttcacca accagaccgt cctgatcacc gcctcgtcgg gcaccggatt atgggaagcg
    20281 gccgtgcgca actgcatcaa caagaaagcc ctgacctgcg tcaatggcgc ttttgcctcc
    20341 cgcttcagcg atgttgtcgg cctgaatggc aaggatatgg aaatcctgtc tgtcccttgg
    20401 ggccagccgg tcctgcccga gcagatcgtt gagcggctat cgaccggcga attcgacgcc
    20461 gtaaccgtcg tccacaacga aaccagcacc ggagcgacca gcccaatcaa ggagatcgcg
    20521 caggctatcc gggccatgcc gggcggcgaa gatattatga tcctggttga ttccgtcagc
    20581 ggcctttccg gagcgcgaat cgagatggac gcctgggatc tggacgtcgt gttgtcgtcg
    20641 agccagaagg cgtttgcctt gcccccgggc ctggcctttt gcgccgtctc cgatcgagcg
    20701 atggccaagg ccaaaacggt ccccgcgcgc ggctactact tcgacttcat cagcctggcc
    20761 aaatccatcg ccaagaatca gactccggca acgccggccg tctccttgct ctatgcgttg
    20821 gatctccagt tggacaacat gatggccgag ggtatggaaa accggttcgc ccgccacctg
    20881 gccatgcgcg atcggacgct tgagtggtcc aagtccaagg gatttgagat tttcgtcacg
    20941 cccggatacg agtcgccgac ggtcacttgc ctggccaata acctggaaat cgacatttcc
    21001 gccctgaaca aatatctgcg cggccacggc atgatcatct ccaacggcta tggtgatctg
    21061 aaaggcaaga cgctgcgcat cgctcacatg ggcgacatac aaatgtatga gatggaagaa
    21121 ctattcgccg cgatggacgg tttcttcgct taagtgtgat tttagatgac caacaagacc
    21181 tgacgggtcg gcgaaaaaac accagtcacc caggacatag gctgccggcc tgttaggtct
    21241 ctgtagatag cccagaacac catgtaccga atattggtat ccgacaagct cggcgatgcc
    21301 gcgctggaaa agctcgggga attcaatgat gtaaccgttg acgtgttact cggacatgat
    21361 caggatcaat tgctggaaat tatccccaac tacgacgccc tgatcgtgcg cagcggcact
    21421 caggttacgg ccgatatgtt ggcagcggca aagcgcttgc gcgtggtcgg ccgcgccggc
    21481 gttggtgtgg acaatatcga tgtcagggcc gccacgatga acggcgtcgt ggtgatgaat
    21541 acgcccggcg cgaacagcat agcgaccgcc gaattgacgc tggggttgat gctggctgcg
    21601 ggtcgtcaca tcccgcaagc ccatgcctcg ctcaaggcag gcgaatggcg gcgcggcgat
    21661 tttgtcggca cccagttata tgggaagaca ctgggcgtcg tcggtttcgg ccgcgttggc
    21721 aagcttgtca ccgagcgggc caaggcgttc ggcatggata tcctggtctg tgatccggcg
    21781 atcatggaag agacggcgcg tgacctcgga gttttgctgg tcgaattgga cgacttgctg
    21841 gccgaatcgg atttcatcac cctccatacc gcattggccc ccgcgacgat cgggctaatc
    21901 aacgccgaat cgatcgcccg tatgaaggat ggcgctatct tgataaacgc cgcccgaggc
    21961 aagttgatcg atgaacatgc agtggccgat gccctcagaa gcggcaagct ggcggccgtg
    22021 gctctggatg tttactccac cgaaccaccc gacgacggaa acccgttgat cgacctgact
    22081 aacgtaatcc acacacccca tatcggagcg agcacgcacg aagcggagcg tgatgtaggc
    22141 atccagatcg tcaatcaggt ggtgtcggcc ctgcgaggta ccgatttcac ctacgccgtc
    22201 aatctgccgt tcgacgttat aggcgacttc gcggatatca agccctatct ggatttggcg
    22261 gagacattag gccggcttca cgccgggttg gccgacaaaa cagtaaagcg aatcgagatc
    22321 gaagtccacg gcgatgtggt cggcaacctc gtccgtgcca tcggcgcagg aatgctgaaa
    22381 ggcgtcctcg ctgcgacaag caatgtgccg gtgaactaca tcaatgctcc caccctcgcc
    22441 aacgagcaag gcatcgttac aacccaggct gtcggcctca ataaccttga ttaccccaat
    22501 ctgatagcca gccgtgccat ctggaacggc ggcgagcgcc tgctggcggg cgttttgttc
    22561 ggcggcagcg aggctcggat cgtgcaaata gatgagtatc ggctggaggc caagccggag
    22621 ggtctcgtgc tggtcatgca gaatctggac gtacccggcg tcattgggca agtgggcacc
    22681 ttgctggcaa atcacggggt caacatcggt gaatggcgtc tcggtcgggt ctgtcctgga
    22741 ggtgaggcga tgtcgttcat caatttggac accctgcccg gcgaggacac tctgcgcgaa
    22801 ctcgccaaca tcgaagctgt gacacaagtc agggtagtca ggctttagca ctaaaccgac
    22861 agctgaaaca aaaaaaggcc gcgcgaaaat cgcgcggcct tttatttctg aaaatgtaat
    22921 cgggttactt cttgggaata atccgaacgc ggcgctgctt gccctcgccg gcgctctcgg
    22981 tatacacgtc ggccgaattc cgtagagtca tgtggatgat tcgccgttca taagccgaca
    23041 tcggctccag cccaatcggc tcaccccgct gccgcgcctt ctcagccatg cgctccgcca
    23101 gtcgcgtcaa ggcttgttcg cgccgctggc gaaagccctc aatatcaaca acaaaatttg
    23161 aacgccggtg cagacgatgg gcgaccatca ggcggctgag aaattgcagc gaatccagcg
    23221 tctccccacg cgggccaatc agcacgccca gatcgtcacc cttgatttca atgatgttca
    23281 cacgctgtcc ggtgaggtca tcaggctcgg tcacatatcc actgacctgc cctttgacgt
    23341 gcattttgtc cagcagttcg gtgataacct cgatggctgt ctgctgttcc gcatccaggt
    23401 tttcctcaga agaagatgat gtgtcatcct catcctcgtc gtcgaccggc tgggctgcgc
    23461 gcgcctgcgg tcgttcgatc ggcttggccg gcggtggcgc aggcttgggt gcttcccgaa
    23521 cgggcgcggg ggtaacaggc ggtttgggag cttcctctac cggagtaacg ataaggcgga
    23581 cgattgcctc gcgactgcct atccccaata acccgcggct gccttcatca atgacgatga
    23641 tcttcactcg atcacgcccg acgccaaggc gttcaagccc cgcgttaacg gctgcgtcaa
    23701 cggtgacgcc gcgagattca atttcctggt tcgaagataa tgccatgatc tatcaccgtt
    23761 ttgcggatcg tttcttccgc ttcgtcgtgg tctggctgga agaagacgat ttactcttgg
    23821 ctgcacctga tccattggcc gattggctgc tctttgttgc agctttactg cccttctcat
    23881 catcggcatc aatcacataa ggcgtgccgc cattcgcggc gcgctcggcc ttttccctat
    23941 cggtaatccg ccgcatgtag agaccctgga agataccaat gatgttagcc atgatgaagt
    24001 aaatcgacaa tcccgccggg aattgcagcg agaaaaagcc aaacatgagc ggcatggtgt
    24061 actgcatcga ctgggtcatc gcggcggccg gattgtcctg ctgattttgc cccttggtcg
    24121 aaggagtcaa cagcttttgc tggatgaaca tgctggcaaa caccacgata ggcagcacga
    24181 aaaaaggatc cggctggctc aggttcatcc agaaaaactt gttactgacc gggatcaatg
    24241 gcgtcaggtt gatagccgaa taaacccgcg ggaccaattc aaacagtgat tggggagtcg
    24301 aacccaggac gatgatgatc gcctgataaa gaccgatgag gatggggaac tggatcaacg
    24361 tcggcaagca accggtcaat gtgtccgccg ggttataccc gatcctccga aactcctcct
    24421 gcatcttctg ggggttgtcc cgatatttct tctggatggc ctgaatctgc ggctgtattt
    24481 cctgtgtccg catcatcgag cgctgctggc gcatattcag cggcaacgtg atcaagcgga
    24541 tcacgaccgt gaaaacggcg atcgccagga taaaattgtt gcccagcaag tcataaaaga
    24601 agagcagggc gttgatcatg gggttgacga ttagggaatc ccacatggcg gcgttctcct
    24661 gcgattaatt atcgctctcc ggcagtgaat agcgccagag ttcctggccc gtcgccaggt
    24721 cgaatccgat gagcagggca tccgcattct gcaaccccac aacaatggtc ttctcaccta
    24781 caacaacagg cgtcgccaga aggggcgcag aagttggctg tgaccatttc tgcgtgccgt
    24841 cggccgtcga ataagcggtt aacgcgcccg aaggcgtggc gcctccgttc tgcgaggcga
    24901 tatagagcgt gtcccctacc accacaggcg ctgtctggat acgattgccg gttgactgcg
    24961 tccaacgctg cgctccactc tccgcatcag cggcgtggat gttgccttca atatcgctgt
    25021 aatagatgac gccatcagca aatgccggcg ctccccagac ccagcctgaa gcgggagcag
    25081 accattttac ttcgcccgag gcgatctcaa gggcatggac gttgccgtca aagctggaga
    25141 cgtagagcaa atcatcgccc agtaccgcct ggcccgccaa agcgccgccc aatcgggtct
    25201 tccagagcag ttcacccgta tctgcgtgca aggcgtagac ggaccaatcc attgagttga
    25261 tatagaggat gccatcacga aaagaaggct tcccccagat ggcatgcccc gcctcgtaat
    25321 cccaggtcac gctgccgtct ttggcattca aagccaggat gtggttatcg gctgtgccca
    25381 catatacttg gtctcccact tgtaacggcg gcgcaacaat tcggtcggtc gcggcgctcg
    25441 aattgatcca tagctctttg ggagtattcg tattagcgtt cccgagtgca tatatggaaa
    25501 cggtcacgcg aggcgacaag aacccgccgg cgcggccgta atcaccgaaa acgacctgtc
    25561 cctcttcggc cgagggagcg gaatagaatt gaactgtgtc agcctcagca ggataaatcc
    25621 actgccgggt ttgggtagcc ggatcatagg caagtacccg cgggccgaaa gcgaggtaaa
    25681 ccaggctccc gtccgtggac aaacccgccc agttggtatt ggcgatgcga ccgctacatg
    25741 cggtcaaaaa catgaccccc acagctataa tcagcagcca aagcgcgggc cgccgcagcg
    25801 gccgtctcaa ttgttgcaag gaacacctcc ctcacggaac cgggtcatag ccgccagggt
    25861 tgaacgggtg acagcggctg atacgcttga tcgccatcca gccgcccttg aagaacccgt
    25921 atttctcaat tgcctggtac ccatagacgg aacacgaggg cgtaaaccgg cagcttggcg
    25981 gcagcactcg cgagatcgtg ttttggtaca gcttgataag tagtaatgct atgtgtttca
    26041 cgacagtgat ccttcaaccc cggaggtcgg ttactaatca atcaggactc cgcttcggga
    26101 caatagctca atcaatgcgc tttccagttc gctatagctt gctgtaacaa cgcccgatcg
    26161 agcgacgata agacaatcgt aacccgccgc catcttgtcc agtcgggcac ggagaatctc
    26221 ccgtatcctg cgctttactc gattgcggcg agtggctttg ccgatgtgac ggccgacggc
    26281 gatggcaaaa cggctcgccg cggcgccctc ctgaggttga ctgtaaacga acaaggcaac
    26341 aaatggatgt tgccggcgct gcccctcccg gcggacaaga ccgatttcgg ccgaattccg
    26401 caagcgatgc tgagaaggca gcatgaaagg atcacatcat catgaacaat tattagccgc
    26461 cgccataacg cttgacggaa cgatatgccg tggccgacgt gccgttccag ttcacgcgct
    26521 tggtgtggct gttcatggaa acagacaaca cgcgacggcc gcgagcgcga cgacgcttca
    26581 ggacatcgcg tcctcccttg gtcgacatgc gcgtgcggaa accatggacg cgcatccggc
    26641 ggcgaatttt cggttgatag gtacgtttag tcatctcaca ctctccaaaa aaacagggcc
    26701 tactgccctg taaatcaagc caattgattg aaacctgctc aggtgcctcg ccggaatgcc
    26761 agggaagcaa tgcaccatta tagcgtaatc ggtgaagcgt ggtcaaatcc cggccgccca
    26821 aaaacggccg gaaatgccgg ttctaataaa aataacccgc ctgttgaaaa ttcttctact
    26881 cgattctgct gatttcggct cccaggccac gcaatttggc ctcaattcgc tcataacccc
    26941 ggtcgatctg ttgaatattg ttaatagtgc tggtgcctcg cgcacaaagc gcggccaaca
    27001 ccatcgccat gccggcccgg atatccgggc tggtgacgcc ggaggggttg gcaaacaatt
    27061 tggatggccc ctgcaccaac acgcgatggg gatcgcacag cactatcctc gcacccatga
    27121 acgtcaggtg gtcgacaaag aaaaagcgac tctcatacat ccagtcatgg agcagaatag
    27181 agccggcggc ctgggtaccc atcacaacgg caatgctcat gagatccggc ggaaaaccgg
    27241 gccagggcaa cggcttgatc tccgggatgc ggccgcccag accttgctgg atggccagct
    27301 cctgatcgcc cggcacgatg atgtcttcgc cgtcttcaag ccaactaatg ccaaaccgtt
    27361 tgtacacgag gcggatcatg cccaaatttc ggggctgggc atcgcgaata cgaatctcac
    27421 ccttggtgac ggccgccgcg ccgatgaaac tacccacttc catgaagtcg gacccaatgc
    27481 ggaactccgt tccgcacaat ttgtccacgc cgctgataga cagacgattc gtgcccacgc
    27541 catcaatccg cgcgcccatg ctgttcagga actcgcacag gtcgcgcaca tgcggctcgc
    27601 aagcggcgtt gtcgataatc gtttcaccct tggccaaaac agcggccatg acggcattct
    27661 ccgtcgcagt cacgctggcc tcggccagaa gaagccggcc ggctccatgc agttgtggag
    27721 ctgccatctc aaacacgccg cgctgggcca tactcacgct tacgccaagg gcagtgagag
    27781 cctgcacgtg tgtgtcgagc gggcggccgc cgatcacatc accgccggga atgggcagcg
    27841 ccacacggcc catccgcgcc aacatgggcc cggcgaacac gaatgacgcc cgtgtgcgcg
    27901 ccgccagcac cggatccagc tccgtcttgg tgacgttttc ggcgtggatt cgccagcttc
    27961 cttcgcccag atcggtcatc tccacgccca gatcgcgcag gatcagtatc gtgttttcta
    28021 cgtcccgaat attgggaata ttgtggagcg taaccggttc gtcagtcagt aaacaagcgg
    28081 ggagcagctt aagcgcggcg ttcttgttac cgctggcagt cacctctccc ccaagcggca
    28141 agccgccctc aataataaat ttactcattg ttcaatcccg gccggatatt ttccggcaat
    28201 tccagccatt gtatcaaccc ccaaacggga ggcaaacgag ctataatctt ctcatgccca
    28261 tcattaacat gaacggtcga aatctgcatt atgacgaaaa aggcgacaaa ggcgctgatt
    28321 tcacgcttct cttcatccac ggagcgggcg gctctttgga cgtatgggcg gaagaaacca
    28381 taacgttgaa aggatgtcgg tctattgcgc tcgatctccc cggccatggg caatccgatc
    28441 cgccgggcag gcgtaccgtt gcccactatg ccgctattgt cgaggagctc atcggctcca
    28501 tggcactgga gcgcccaatc ctggtcggcc attcgatggg cagcgcaatt gccctgacta
    28561 tcgcccaccg aaaactcgta tcgctccacg gccttatcct gatgggcggg agcgccagga
    28621 tgccggtcgg cgcgccaatc ctgtcaggcg cattagctga tttagaggga gtcgccacgt
    28681 ttgtggccga gcatggggcc gtccacatca cggatgaatg gcgggaagtg attcgccggc
    28741 aggtcatggc caccggagaa atgacgactt atggcgacta cctggcctgc aaccggtttg
    28801 atctgaggct tcatctatcg gacatcaacg tgccaactct attcatcgcc ggacggcagg
    28861 acagcctggt accgctacgc ttctcggaat cgacggccgc ggcgatgccg cacgcgcgtg
    28921 tcgctattct ggaagacaca ggacattttg tcatgcttga gagaccacaa gcctttcggg
    28981 gaatcaccat ggggttcctg catgatccgc tagtcgcgac tcgttagagc ttacggcttt
    29041 cggccattga gcacctctcg cagaagggga gcgatggctt ccatagcgcg gccgcgatga
    29101 ctgatgcggt gcttttctgc cggccgaagc tgggccatcg tcagaccgta ttcggggaaa
    29161 tagaaaatcg ggtcatagcc aaaaccggct tccccggacg gctcccaaac gatttttcct
    29221 cgacacacac cctcggacac gccgagaatc gtcccatccg gcgccgccag agcaatcacg
    29281 cagcaaaact gcgctgccct gagcgagtcg ggtatctccg tcatttcttg cagcagcaag
    29341 gtgtaccgct ccgccatgga aaggcccgga ccgccatagc gagcggtgag aacgccgggc
    29401 cggccgccca gcgccgcaac ttccaggccg gaatcatcgg ccagagtcag cagccccgtt
    29461 tgcccggcat aggccgtggc cttaaggata gcattctccc gaaatgtact gcccgtctcg
    29521 ggcacatctt cagtgatgcc gacatcgctc aacatgagaa aggcaatgcg gccgtttcca
    29581 agaatgtcgg tatactccgc tatctttcct ttgttgtgcg ttgctaccag caacttcctg
    29641 tcgcttacct tagtcacggc tgataccctt atacgccggt taaacattca tcaccggcta
    29701 tgttataatc cgattttatc ccgaaccgag ttggaaggtg tatcatgaaa ctttcacgcc
    29761 tgcgttttaa ttttggcttt ctgttagagg cggattatgg caccatccgg cagatcgaac
    29821 tcgactatcc cagcatcaag ctcagcgatg atgtgacctt aaccccattg gtggggttta
    29881 ttacagctac gcgcacgacg gaaggtattt acattcaagg acaattggaa agctcgatga
    29941 gcctggagtg cgtgcgctgt ctggaagaag ctatcgtgcc cgtgaatgtc accctggatg
    30001 agctgttcta ctatccaccc cacatcgcgc cgccgggcga acagcacgtg ggcgaggacg
    30061 ggatgatcga tctggccccg ctggtgcgcg agctaagtct gttgagtgtg ccgatcaaag
    30121 tcctgtgccg gcctgattgt cagggcttgt gcctggaatg tggcgcaaat ttgaatttag
    30181 gggaatgtgg ttgcgagacg gaagaggtcg acccacggct ggccgtgcta cagtcacttc
    30241 tgaagaacga cccagccaac aactagttac aggacatatc cagggttttt atgttttacg
    30301 acgaagcaaa aatccacatt cgcagcggtg acggtgggga cggcatgatt agttttcgcc
    30361 gcgagaaatt cgtgcggcaa ggcggccccg acggcggcga cggggggcgc ggcggtaacg
    30421 tgatcttttc cgcctccagc catctgaaca gtctggccaa tttccaacgc aacaaacatt
    30481 atcgggcagg aaatggtgtg catggaaccg tccagcgaat gaacggcgcg agcggcgatg
    30541 atctgcgact tgaagcccca cccggcacga tcatccgcaa cgctgagacc ggcgaaatcc
    30601 tggccgacct gatagagccg ggacaggaag tagtcattct gtcgggcggg agaggcggcc
    30661 gcgggaatca gcatttcgcc accagccgcc atcaggctcc gcgaatcgct gagcgcggcg
    30721 agccgggcga agagttgtgg atcaagctgg agttgaagct catcgccgat gtcggcgtga
    30781 tcggcgtgcc caacgcgggg aaatcgacgt tgctttccgt cgtcagcgcc gcgcgcccaa
    30841 aaatcggcga ctatccattc acgactctgc aacccaacct gggtgtggcg gcaatcgacg
    30901 attacgagac gatcgttctc gcagacattc caggcctcat cgaaggcgca tccgaaggtg
    30961 ttgggctggg ccatgacttt ctgcgccaca tcgagcgcac gcgcgttctc atccatctgc
    31021 tggatggcat ggctgaagac ccgctggccg acttcaggat gatcaacaaa gaactggccg
    31081 cctacgacgc tgaactggcc gccaaaccac aactggttgt cctgaacaag atcgatctgc
    31141 ccgacgttcg tgcccgggaa ggcaaaattc gtaaggcgat catgaaggcc ggttacccgt
    31201 ttatgagcat ctccgccgtc accaatgaag gggtgaaggc cctgctctac gaggtcaagc
    31261 gcatggtcga cgccacgccg cgcgagctgc cgacggcgac catcagcgag ccggtcgtca
    31321 ttcgcccacc cgtcgaacag caagccttca ctatcaaacg ggaaggcggc aacacgtggc
    31381 gagtgcgcgg caaggatatc gagcgcatcg ccaccatgac ctacttcgaa ttcgacgact
    31441 cgctcctgcg attccagacg gtcctggaga agtccggcat cacggccgcg ctgaccgaag
    31501 ccggggtcaa ggtgggcgac acggtctata tcggcgatca ggaacttgag tggggtgagt
    31561 aacctccacc cttgccggca atgaccgatt ctcccgacat tctcctgcgt aaagctatcc
    31621 tcaacaacga ggagtttgtt gaggctgcct tcctgggcac acgaggaaat cagacaaacc
    31681 cgtgggcgcg agtgacgatt cgccccgtcc tgctgaaaga gcaacacctt ttgcagtttg
    31741 aatacttcga cagcaaacaa gccttcgcca aaaattaccg cgaagccgaa gctgaggaag
    31801 cattgatggc gctcgtggcg ctgccgttca aaaatgtgca actctccaca accgagggca
    31861 cggttcgaat tcaatacagc aagaaagggc ggccgatcat ccaccacgac gctccccgac
    31921 agaagagggt tgacctgggc ctgacccacg atcgggtcaa gccggggatc ctgagtgaag
    31981 accaggccgc cccctttttg aacgcgatcg gcgtaaccac ggccgatggg catgtgcgcg
    32041 cggatcaaca gcgcaagttc cgccagatca acgagttcct gcgcctgatc gacgagaccc
    32101 acgagatgga gcatttcggg cacagtccgg tgcacgtcgt ggatcttggc tgcggcagcg
    32161 ccgccctgac tttcgctacc tatcattacc tgaacaccat caaaggcatc ccggccgcca
    32221 tgaccggcgt cgatacaaaa acatatctgc tggaacgcca tcgggagacg gccgtccaat
    32281 tgggatggac gaccatgact ttcgagccaa gccggatcat cgatttccag ccggcggaat
    32341 cgcccgatat tgttctggcc ttgcatgcct gtgataccgc caccgatgag gcgttggccc
    32401 aagccatcag gtggcggagc aagctcatct tcagcgctcc ttgctgccat catcatctgc
    32461 aagcccaact gtcccaggcc accacccccg aacccttcag gcctgtcctg cgccacggga
    32521 tcatgcgcga gcgcctggga gatgtcctga ccgactcgtt cagggcgcac atcctgcgct
    32581 tgctcggata tcgggcggag atcatggagt ttgtagcggt ggagcacact cccaagaacc
    32641 tgatgatccg cgccgttcgg atggacaccc caccaccgcc ggcacttgtc aatgaatata
    32701 tcgcgcttaa agcctactgg ggcgttacgc cttatcttga ggagttattg cgcgaccaac
    32761 ttgccccaat cctggccgca gcctagatcg agcggccaaa cgcacaagga gtcattctct
    32821 atgccggcaa ccttggtact cacgaacgga cgaatttaca cgatcgatcc ggcacagcca
    32881 caggccacag ccgtcgccat ccgggacggc cgctttctgg ccatcggcag cgatagcgag
    32941 atgaaggctc tcctgggacc tggtggcgag gtggtggatc tgggtggccg gtttgtgaca
    33001 cccggcctgg tggatgccca cgtccacttc cagcacttcg ccctcagcct gcaaaacgta
    33061 gacgtagaag gcgcaccaac ccgacaggca gcgctcgagc gcatcgccgc gttcatcgag
    33121 caacaccccg gcagcggctg gctattcggc cgtggctggg tgcaggacgc ctggcccgat
    33181 catgctttcc ccacggccgc cgatctggat gacatcatcc cggatcggcc ggtctattta
    33241 tctcacaaga gcggccacgc cgcgtgggtg aactcgctgg cgctcaggat cgcgcaggtg
    33301 aatgagacga cgaccgatcc gccgggcggg caaatccagc gcgatgccac cgggcgcccg
    33361 acagggatcc tgttcgaaga tgcgatggat ctggtcgcgc gtcacatccc ccgccccacg
    33421 gtggacgaaa tcgctttggc tatgcgcagg gcgcaggcat attgctggtc ggttggcctg
    33481 accggactgc acgacttcga cgggcgcgac tgcttcctcg ccctgcaaca attgcatgag
    33541 gcggatgaac tggggttgcg aatcgtcaag aatgtgccgg tctatcgcct ggagcacgct
    33601 ctgggcgtcg gtttgcggtc gggctttggc gatgactggt tgagaattgg tggcgtcaag
    33661 atcttcgccg acggcgcgct gggctccagg acggcggcga tgatcgcccc ctatgaaggc
    33721 gatccgggaa atgtgggcat cgtcgtcaca gacaaagagg aaatgctgga gaaggcgcgc
    33781 caggccggcg ccggcggact cagcgtgacc gtccacgcca ttggcgatcg ggctaaccat
    33841 gatgtgctgg atgtttatga actcctgcga aaagaggaag cgaatcgggg tggcccgcga
    33901 ctgcgtcacc gtatcgagca cgttcagata ctccatccca gcgacctcaa ccgtctggcc
    33961 gagttggacg tcatcgcttc gatgcagccc gttcacgcca cgtcggacat ggaaatggcc
    34021 gaccggtatt ggggggagcg cacgcgctat agctacgcct ggcgaaccat gtgggatagt
    34081 ggcgcgctgg tggtgttcgg ctccgatgcg ccggtggagc ggatcgaccc gctgccgggc
    34141 atccatgcgg ccgtcacgcg acgaagagcc gacggctttc cgggcaagga tggctggcat
    34201 ccgcaggaac gcctgacgat gggggagacg ctccatgcct tcacccaggc agcggccatc
    34261 accgcagggc aagcgaaaac acaggggagc atttcgcccg gcaaactggc cgatctgacg
    34321 atctttgaaa aagacctgtt cgagacgccg gccgatgaac tcctcaatgt tggaatagcc
    34381 gggacgatcg tcgggggaac cttcaggcac cgtacctggt aacgcccaaa gtaaaaaagc
    34441 cgagttacct ttcggcaact cggctctcaa attcgttcga accttcttat aacacgttgc
    34501 gtttggtctc ggcgacaagg tgatcgacga cggcacgcat ggcctcgtcc tgattcccgt
    34561 ccccgccatg ttcgtaatag acccgcagct gcttgtcggc gctggtgccg tcgcgcagga
    34621 tattgcgggc gtgttccact tcccggcggc tgtccagctc atcgaccacg tcgtcgagaa
    34681 actccagcaa ttcgccgatg agctggggaa cgggaaccga agcctggatc ccaaagtcaa
    34741 tcagctcgcc ctcaatacca tagcgcacgg cccgccactt gttttccgtg atgtggacgt
    34801 gacgatattg ccgccaggac atattctgcc tgcgcagctt gagcagcttg gcacaaatcg
    34861 cctggaatag cgcggcaatg cagatgcaat cgtctacgct ggtgcagata tcgctaatgc
    34921 gaaactccag cgtgctatag accgggtgcg gccgaatatc ccaccagatt ttggctcgtt
    34981 tatcaaactt gccgaacgcc ccaacccgtt cgagcatctg ctcgtatgct ttataatcgg
    35041 cccaggaaat gaacgagtgg gggatacccg tgcgcggcaa tgactcaaaa accacgctgc
    35101 gataggactt gagacctgtg ttttgcccgc gccagaatgg cgaactggtt gatagtgcca
    35161 gaaggtgggg gatgaagtag cgcgcctgat tcatggtgtc gatgagcatt tcccgcgaag
    35221 caggatcgtc accgaaaccc acgtggacat gcatgccgaa tatcagcaac tggcgcgcca
    35281 cgccctgcat gtcatccaac agttccttat atcgagttcc ctcagtgatg atctggtctt
    35341 cccagcgggc aaaagggtgg gtcgaggcgg cggcgatcat cagcccattc tcatcagcca
    35401 attccagcac atcccggcgc aggcgggtaa tctcggcccg cgcctcactg atattttggc
    35461 agatgcggct gcccacttca acttgtgatt gcataagctc cggcttcaga tcgagcttat
    35521 gttcgcgggt ctgatcctgc gtaagaagct cgctgatata cgtgcgcaaa tcacgggttt
    35581 cggcgtcgat gatttgatac tcttcttcga tgccgagggt caaaggttgc tctggaactg
    35641 ccataatgtt tcctcaaacc aggctcatca tgtatagaac cccgcaatca acaggcctgg
    35701 ttattggacg aggattatag cacgtagcaa tcggcccacg taattacaac cctcgcccga
    35761 tactcatcaa tctatcgtca accagccatt ccctgcaaat aggcgatcag gagacgcacg
    35821 ccaaaaccgg tgccacccac cggggaataa tcaacagcct tttcagacaa cgccatcccg
    35881 gcaatgtcaa gatgggccca ggaatagttg gtaaactcgc gcaggaagac ggccgatgtg
    35941 ccgaccccac ccatacggcc gccgctgttc ttcatgtcgg ccacttcgga gttgatcgtg
    36001 cggcgataat catcatagag cggcagcggc cagatgcgct cgtaagtcgc ttcggaagcg
    36061 gcgagtagtc tatcgcgcaa tgagtcatcg ttgcagaaga tgcccgcggc cacgccaccg
    36121 cccagcgcga cgacgcacgc ccccgtgagg gtcgccagat cgatcacggc atcaggctta
    36181 tatgtttcgg cgtgaacaag cgcgtcggcc aacaccatgc gcccctcggc gtcagtgctg
    36241 atgatctcga tcgttttgcc atttgatgcg gtgatgatat ccgacggccg ataggcgttg
    36301 gcatcgggca tattctccgt gcaaggggtg atgccgatga cgcgcagcgg cagatcgagc
    36361 cggccgacag tctccatggc acccagcacg gccgccgcgc cgcccatgtc ggacttcatg
    36421 ctgcccatgc cctccgacgg cttgatggag atgccgccag tgtcgaaggt aatccctttg
    36481 cctaccagaa cgacggtcgg cagatcggtg cggtctgcat tgtgctcgag gacaacgaac
    36541 tttggtggtt cgcccgcgcc ctgagccacg gccagatacg cgcccatctt gcgctccccg
    36601 gcccactcgc gatcgccgat gatgaccccc atgccgtgcg cgccggcaat agcctgagcc
    36661 gcggcggcca gctttgtcgg cgtggccaca ttgggcggca tattgaccag atcgcgagcc
    36721 agatacaccc ccgcggcaac ggcctcggcc gctttcgcgc cggcagcaat agcatcaata
    36781 cgggaattgt cgaattcaac caatgtcagg gagccgattt cgttcgccgg ctctctcttc
    36841 ttcggcgcgt cgaagcgata agtggacagg agcgcccctt caacggtcgc ctgcgcggca
    36901 gctttcacat caagaccacc aatgcccgcg ccatggacaa tggtcgccac gtgttgcgca
    36961 ttcaaatcgc gcgcccgctt aatggccgcg gccgacgccc gacgtacgcc ttccaaatta
    37021 aagctgtcgg ctttgcccaa ccccaccacg aggacgcgcc gcgccgcaat agcgccatgt
    37081 gggtaaagca cggcaacttc accggccttt cccttaaagt caccgtcggc gatcaagtcg
    37141 gagatcatgc ctccgagcgc tatatctacg gcgccggtcg ccccaccggg ggcagtgacg
    37201 ccttcaaaaa gattgacaat gatcgtatcc gcgcctgagt gcacaatatt gccgttttca
    37261 attttgatat ccatggtttc tccctgatga ttgaattaat catctgattc ttgtttctcc
    37321 actattggac ttattctctc attaataccc agccggacaa tgagcgcagg gctggtcatt
    37381 cttatccttg atctgcggtt atttatggta acgttgagat tggggtataa tatgaattcc
    37441 cacagatttc catcaaggag tatcccatga tagtcggagt tcccaaagaa attaagaata
    37501 atgaatatcg cgtggctatg acgcccgcgg gcgttcaaca actaattgag caaggccata
    37561 cggtgatggt tgagaccggc gccggcgacg gcagccgctt cctggatgag cattacgcgc
    37621 gaatcggcgc aaccattgtt cccacggcgg ccgacgcctg gagcgccgag atggtcgtca
    37681 aagtaaagga gccaatcgcc gtcgagtatc agtacttgcg gtccgattta ctattattca
    37741 cctatctcca cctggcggcc gaagaagcat tgacccatgc catggtcgac tccggcgtca
    37801 cgggcatcgc ctacgagacc gtcgaagcgc cggacggcag tctgcctctg ctaacgccca
    37861 tgagcgaagt agccggccgc atggcgattc aggtcggcgc ccatttcctg gagcgctctc
    37921 acctcggccg cggcatgctg ctgggcgggg tggccggcgt gccgggggcc aatgtcgtcg
    37981 tcatcggcgg tggcgtggtc ggcaccaatg ccgcccaaat tgcgctcggc atggaagcca
    38041 acgtcaccat catggacaca aacctggatc gcctgcgctt ccttgagcat gtccttcacg
    38101 gccgcctgac aacagtcgca tccaactcct acaacatcgc cgaagcggtg cgcaatgccg
    38161 atctcgtcgt tggcggcgtc ctgatcaagg gcgcacgcgc tccgcgactg gtgacgcgcg
    38221 agatgatctc gaccatgaat ctgggtagcg tcatcgtcga cgtcgctgtt gaccagggcg
    38281 gctgcgtcga gactatccac gccaccactc actccgatcc ggtctacatc gtggatggcg
    38341 tcgtccatta tggcgtcgcc aatatgcccg gagccgtgcc gcgcacgagc acgtttgcct
    38401 tgagcaatgc caccttgccc tacgtcctgc gtctggcgcg ggtaggcgcc gaagccgcga
    38461 tggccgaaaa tcacggtttg gctctgggtc tcaacaccta tcagggcaag gtcacctaca
    38521 aggcggttgc cgacgcattc gggctggaat acacgccgta ccaggcataa caaaaataca
    38581 catcccactg cccctgatgg aatcctccat taggggcagt gacgattggg cctctgccta
    38641 agccaacgtg gatctactcg ccatcaccgc ccaactctcg attataaacg ggacagtcca
    38701 ggacgaaggg actgtcccgg gcttgttggc gcaacccgcg ccacagaaag cagcgcgcgg
    38761 acgggagcgt gactatctct tcatccatct cgcgctgaca ggcataccgg aacagaccgc
    38821 tgaactcgct cgtagccttg tcgagggatt acgcacgcga ttttttgccg cgtcgggcag
    38881 cgtgacgtct gccttgagac gcgcggtcat cgagacaaac gaagagctat tgcgatttaa
    38941 tgtggaaaag cgtgccacgc atgagggcgc gctcagttgc gcggtcttgc acagcggcga
    39001 gttgtacacg ctacagacag gcgaaggact ggcctttttg ggccataact ttggtgttga
    39061 gcgcctgccg gctcgcccgc cacaacacct cacaccactc ggtcgcagcg ccggcatcga
    39121 cattcgcttc gccttccatc ggttgcagcc cggagatatg atgcttctgt gcgatccgcg
    39181 cctgtcctat ctgaccgggg acacgctggg atcggtactg gtcgataccg agatcgaaag
    39241 tggctttgag gcgttgatgg acgttgtagc cggggacagc gccagggtca tgctggtgga
    39301 attcgccgac gaactgccgt ccaccctgcc acttacattt cagcacagca agcagcctgc
    39361 tcccaagccg gcaacgacca aggcacaatt gaagcccgca tcggggaagg cgatccctgt
    39421 cagggaggcc acaaccgaga caacgactcc cgtcgttcag gaagagccat ctccatcatt
    39481 tgacacgacg gcagtcgaga cgagcgcccg tcgtgccgcc tcatcctccg ccagaggatt
    39541 gtcacgattc atcgcctgga tagcggccgt cctggagcaa ttattgggca ggcgaagcga
    39601 cgagccgtcg attcattggg ccatccccac taccgtcgcc ctcattatcc cgttaatcgt
    39661 tggctcggtc gttaccagtg tgtacatcca gcgggacacg gtaaaccagc tgtcgcaaat
    39721 caagcaacag atgatggatg agttggcaac cgcggaaagc ctccgcgatg atgccgctgc
    39781 agctcagggc cattatctca ccgttgtctc gttggccggg caagccgaaa cgctgcggat
    39841 tggcgacatc gaggtcgcca caatgcgctc cgatgctttg gatggactgg atcgcctcga
    39901 cggtgtgagc cgcctgacgg caacgacttt ttatcgttat gcagccggca cgaacctgac
    39961 ctccctgact ttgcgcggcg tcgacggagg tattgccgtc ctggatcaat cgggtaatcg
    40021 cgtttacttc catccgaccg atgaaacgtt cgaagagatg accagtgaag agcctcagac
    40081 cattggtttc ggcgggcaat cggttggcgc caacacaata aagaccttgt tcgatatccg
    40141 ctggctacag ggcagcaccg cagaaacgcg cgactcgatc ggcatgctgg acacgacagg
    40201 cggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggcag caccgcagaa
    40321 acgcgcgact cgatcggcat gctggacacg acaggcggtc tgttcaacta ttatcccaat
    40381 ttgggagata ccagcgggat cacgttgggc aacagcgccg tatgggttag ccccacggcg
    40441 atgtctacct atctggatcg cctctatgtg cttgatacag gcgcgaggaa gatttggaaa
    40501 tattacgcgt catcaggcta tgcccagttc gaaggggacg agacgatctt cttcagtgat
    40561 gaagcgaatt tggagcaggc gatcgacttc gatctctatg ccgatgacgg aagcctgctc
    40621 gtcctgtata aagacggcca aattcgctac tacgacacgc actcgggacg cattgtgtgg
    40681 gatgaaaaca cgctggccca gaacgggttg atgacgcccc tcatcgcacc ggtatcggtg
    40741 gaaatcgtcg gcaccggcct taaagcgtcg atttttgttc tcgatcccgg tagcggccgc
    40801 ctccttcaac tctctcgtgg tggtatggcg ttggcccaat atcgcatcct ggatgcgtcg
    40861 gggaatgacg tgttgacaaa ggcggccgat ttcgcggtta ccgactcgcc gatgaggatc
    40921 tttgtcgtgg cgggggatca gctgctggta gccgaaaggt aaaacaggct ctaactgccg
    40981 atgaatttcg gctctcgctt ttcaacgaag gcagtgatgc cctcgcgatt atctttgctg
    41041 cggccggcta cttcctgcaa atgcgcctcg tactccagcg cttcatccaa tgtcatatcc
    41101 caactgcggt gcagcgaccg cttggtcagg ccaaaggcca gcgtcgggcc atcggccagg
    41161 cggcgcgccc aggcggcggt gatctccggc agttgttcgg ccggcaccac atagttcacc
    41221 atcccccact ccatagcgcg cgcggcggga acgcgatcag ccgtcaacgc catctcataa
    41281 gcgcgagcat agccgatcag ccgggggagg aaccagttcg tgccgctgtc ggggatcaac
    41341 ccgatgcggc tgaacgccag cataaaggag gcactatccg cggcgatgcg gatgtcggcc
    41401 gccagggcca cgccgcagcc cgcgccggcg gcgatgccgt tgattgaacc gataacgggt
    41461 ttctccaacg agaccatttg ccgaacgagg cggtggtagc catggcgaag atgctcccca
    41521 atagaaacat catctccgcg accctggaag tcggcaaggt cctggccgga ggaaaagccg
    41581 cgccccactc cggttatcac cacacaacga acatccgcat cgcgaccggc tttcttaaaa
    41641 gcatcagtcg tctcgtctat catctggtcg ttgaatgcgt ttagcctgga agggcgattg
    41701 agggtgacgg tggccacacc atcgctcaca tcatacagga tcgtctcgta gctcatcgcg
    41761 gcctttcctg ttggatagcc tttagaaagg aatgtccgaa tcgatatcgt cgtccatctc
    41821 gcttccttcg tcgacttcct cttcccagag catcgtgacc cagagcagga gcaactcccc
    41881 ctcctcaatt tgggtcaaac gaagggcgac gacatctacc tgctggcgca ggcgcaaatc
    41941 gtccgggtaa tcaaggcgta ccggccggcc ttcctgccat gcctgttgac agcgctccac
    42001 aatatcgctg ccgttgccgg tcctcaattg acacagcgcc acatcagttt catgtggccc
    42061 ttcatatacc gattgcatcc agccctgagg gttcggcatg aaatcagggg tgggtccttc
    42121 aatgatagtg atcagttccg gttccatggg gaatcaaacc tgcgtaagat tttctactcg
    42181 tcggacaagt ctactgagta tatgacggct atgcaaataa gtatttgcct accgccacgc
    42241 cacaaaccat atatcaaagc agcaaatcac aggggctgcc cagcgagaag cgatggttcc
    42301 gtaacaatcg tgaggcatgg aaaatcaggt ttccaagtga gcgccgtcac ggttccgatc
    42361 aaggataagc caggtgaagg caaaccggtg gcgctcatcg gccgggtgct cctgacgaaa
    42421 aacctcgtcc caatcatccg tcttaaattc ggggaaatac acgtcgccct cggccgcacc
    42481 atgaacctgg gtgagataga gacgatccgc cacgggcagc aatcgtcgaa aaagatcggc
    42541 accgccgatg acgacgatct cttccgcgtc tcccgcggcc gcaagcgcat cgtcaacgga
    42601 gtggacgaca actgcgccgg gcgctccgta ttcccgattg cgggtgacaa cgatattgag
    42661 tcgcccgggc agcggccgaa atcgatccgg caacgagtcg taggtcttgc ggcccatgac
    42721 gcatggttta ccgatcgttt gacgacgaaa ccagcccatg tcttcgggca agcgccatgg
    42781 cagatcgtta tcgcggccga tgagccgatt ctcgtccatg gccactacaa acgctatgcg
    42841 ggggatgtga cccatcaaga gcctcctaaa ccgctatcgg tgcgacaatg cgtggatgag
    42901 accggtaatc aagtagctga aagtcgtcat aggcgaaatc gaatatcgac cgaacggcgg
    42961 ggttcagttg catcgtcggc agcgggtacg gctcgcgcga cagctgcagc aaagcttgct
    43021 ccatatgatt caaataaaga tgtgcatcac ccaaagtatg aataaattcg ccgggttgaa
    43081 gcccggccac ttgcgccatc atttgcgtca ggagcgcata ggaagcgata ttaaacggca
    43141 caccgaggaa gatatcggcg ctgcgttgat aaagctggca ggagaggcgg ccgtcggcga
    43201 cgtaaaactg gaacaggcaa tggcagggcg gcaaggccat cttgtcgatc tcacctgcgt
    43261 tccaggcgct gacgataaga cggcgggagt ctggcctggc gcgaatttgc tccaccacat
    43321 cagcaatttg atcaatagtg gtgccgtcgg ccgtgcccca tgaacgccat tgacgaccat
    43381 agatcgggcc taactcaccg tccgcatcgg cccattcgtc ccatatcgtc acgccatttt
    43441 cccgcagata acgaacgttc gtctcaccac gcaggaacca cagaagctca tggatgatcg
    43501 agcgcaggtg catcttcttg gtcgtcagca agggaaaccc gttagccaga tcgaagcgca
    43561 tctgatagcc gaatacgctg atcgtcccca caccggtgcg atcggtcttg gttgtgccgg
    43621 tttgaatgac atggcgcatc aaatcgaggt attgccgcac aaatccacct cccgtttatt
    43681 ctcgcaccga agtataacag catgaatcct tgacggcacg gcggttgatg agcgcggcat
    43741 tgatggttac aataggttct ctgcctcgaa tggggtggtg gcgaaatggc agacgcagcg
    43801 gacttaaaat ccgccggggt aacacccatg tgggttcgag tcccacccgc cctactgcaa
    43861 gatgaatgct tcatgcgaac gctatgagcc tggttgatca ggtattgtct gccctgcgtc
    43921 tcgcttccaa cagcgcccga tttgatctcg accgagacca gatcgttgtc ggcgtgtcgg
    43981 gtgggcctga ttccctggcg ctcctccata ccctgcgcag ccttatcccc gccggccgtc
    44041 tcatcgtcgc ccatctggat catggattgc ggccttcctc tgcggccgaa gcgcaattgg
    44101 tggcccgagc tgcccaggga ctgcgcttct acactgaacg ggtcgacgtg gccgctctgg
    44161 cccgccagaa cggacaatca ctggaggaga caggacgcac tgctcgttat gcgctattgg
    44221 cgcaggttgc tcggcaagaa gggtcaaccc acatcgccgt tggtcacaac agagaggatc
    44281 aggtcgagac gatcctgatc cacatcttgc gcggcagcgg attggccggc ctgcgtggga
    44341 tgcagccggc cggccccttg cctggtcata aggggctgtg gctgctacga ccgctacttt
    44401 ccgtcactcg ggaagaaatc gaaacctatt gcgtcgagaa gcatctcgtc ccgatatacg
    44461 atgagagcaa cagttcgtac tcatttctcc gcaatcgcgt tcgcggcgaa ctgctccccc
    44521 ttctcgaaac atacaacccg caagttcggc cgcgcatcat cgagatgggc gaggtattaa
    44581 cagcagagga agaatatctg gccgcgcaag ctgcgagcga atggagcagg ctgatcatcg
    44641 agcaggaaga gacatatgtc attctgcaac gtcccggctg gttggccctg cccctggcgt
    44701 tacgtcgccg cctgctgcga ttggccatcg ccgccattca accaggcata agggatgtgg
    44761 ggtttcgcgc gcttgaatcg gcgcgggcta tcgccgagcg aggagagacg ggggccgacg
    44821 ccgatttgcc gggcaatatc acgatttcag tcagttataa cagcctgatc gtcaagaacg
    44881 aagcggcgga tcttgccaat aagtaccccc aattattggg cctcgaccca ttaccgttat
    44941 ctgttccagg gaaaaccccg ccggccaatg gatggcggct aaccgcggaa tggctcgacc
    45001 ctggggagtt cgatctgcac gatatccatg agaaccggga taggtggcaa gcctttgtcg
    45061 ttttgagcgc ggccgatact ctgatcgttc ggccgcggct gccgggtgaa cggatgcgcc
    45121 ctctgggtct gaacggcgag cgcaagctca aagagattat gatcgaccgg aagatgcccg
    45181 ctcatttgcg ccggcgttgg ccaatcctcg ccacaaagga tcatgccgtg tgggtcgtgg
    45241 ggcacatcat cgatgagcgt gtccgggtta tcttcgggga acaacgcgtc gttcgtctgc
    45301 aatgcctgcc gcccgaccat catgaggcag gttgacgcgc catcatggag tcagtatact
    45361 aacgaatgag gtcgggtacc ggggcgtagc tcagcttggc agagcgctcg gtttgggacc
    45421 gagaggccgc cggttcgagt ccggccgccc cgactgatta cgtgcggcag tggtgtagtg
    45481 gtaacacaac agccttccaa gctgttttca cgggttcgaa tcccgtctgc cgctcttgag
    45541 attcaggggt agcttcatct acccctctca ttatttctgg agggcctgta gctcaacggc
    45601 agagcggtcg gctcataacc gattggttca aggttcgaat ccttgcgggc ccactcccac
    45661 ctattcctcg ataacctcca ataattgctc caatgctcaa cctctattct ggctgaaacg
    45721 atcatttcgc cggttgaacc gttccccggc aatgcggaac ccacggattt tcggccgtca
    45781 ggcgttcgtc gagaattggt agaggccgat tggtgaacga gtcgtacaaa gccagcgcgc
    45841 aagtaagaga ttgaccgggc acggccgaat cggcggccgt cacccgatgg ggcgagcgca
    45901 cgaacgagcc gtccacccac ttgagcgtgg ggatggcccc catggcgggg atggaatcct
    45961 gcaaatccca ccaggcccaa tgaacaccgt ccgcttccag cccgatcagg cgaacagaaa
    46021 ccacataatc ccgattgatg ggcttcgcgc tatgaaacgc ctgatcaaga accaaagcct
    46081 cccccggctc aagggtcaga tcatcgaacg cttttcctgt ccacacgatg ccttggccaa
    46141 agggaacata atggccacct tccatgccac gcgggaagcg ccaatcggtt atcggcacgc
    46201 cccaaactcc ccggtaagga gggagttgta actggggtac ggctgcatca tcaatcactt
    46261 gcgtataata gccttcaacc gtgcgccaat ggagatagag gcgcgagcga tccgccagag
    46321 tatggtccca atcatagccg accagtgtgc cgggtaggga agtcattagg agacgttcca
    46381 cgacattccg ggtatacggc gctacatctg acaggttgaa aatgattcct gtcaacggct
    46441 cacgcgtgac ggactcaccg gaggttccga tgtacacctg caaagcaccc ggtgatatac
    46501 cggggcgggg cgtgactcga aattgggtca atgtgatccc ttcggctgga ataatcagct
    46561 tgtcatcctg cgcgtagatc acatcatctt gcccaacaag gtgaacgaat aacgtagttc
    46621 cctgcgcgaa tttggctaac ggccgccatg cgaccgtgat cgccgcttcc tccccgatcc
    46681 tcaaattatc aggctggtca acgtgataac ccagaacgtg gatcgaatcc gcaagcgaga
    46741 gttcggcccc gacaaaatca tccggcaggc tttctctcgg catggccgga tagagttgcg
    46801 cctcaccgac cggttcagga accggcaaat cagccaaggg agtcactggt atccaggtgg
    46861 taatcacatc gagaccggct gcatgcgcct ccctgacgcg ccgttcccag gcggcatcgt
    46921 aagattctcc ttcgggaaag acgtattgaa tctcgacgtc ggagcgcaat ccctcgatct
    46981 cctgcaggta ccataacggt gtcacccagt gccagtgggc gaggattgca ctattagggg
    47041 gtgcggactt gagaaggagt tcggtaaatg cgcgggaatc ccggccctca tggaaacgtc
    47101 ccgcagcggt aaggtttgtg actgcctgat agccggccga cacggcaacc agagcgcaca
    47161 ggaatatagc gccgcttcgc ccc
//
LOCUS       DAOMQG010000002        45452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_358_length_45452_cov_7.622200, whole genome shotgun sequence.
ACCESSION   DAOMQG010000002 DAOMQG010000000
VERSION     DAOMQG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 45452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45452
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_45452_cov_7.622200"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="PLC06_00240"
     CDS             complement(<1..1447)
                     /locus_tag="PLC06_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006055828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88905.1"
                     /translation="MFEPNVGDEILIGGYSRFITAHPTAGAMLTPYGQEGRAGTVYQL
                     RDSAGGLTAFKVFNPGFRHPSLVEQVGQLRRFGELPGLSVCHRAVMTPEQQGPLLQQY
                     PELAYAVLMPWIEGRPWNQIIQDRRPLTRHQSQALAEALRTVLAYMDREGVAHGDLSG
                     ANLMIDGLDTGDIRIELVDVEQLYAPGLTRPAKVPAGSPGYAHATIAGRPWQADTDRF
                     AGAILLAEILGWCDERVRAAADSITTNDGKIIPGESYFAEDELQQDCERYRLLCRVLA
                     ENWGADATVLLQRAWNSSALAECPSFSADQAKPLPSPPPSPRQKARTDLMANATAAEQ
                     NGRWGQAVVFYDRLLTGWPGDPATDQWTAARDLARQKAGPPPKPVPPRRIGWRWAAPA
                     LALLLILFGGGAALAGLWPFGGGSEPAPTATMIAAVATESLPTATPGAETAVPPADSD
                     TDGDGLSDSQETQNYNTDPNNADSDGDGLSDG"
     gene            complement(1572..2363)
                     /locus_tag="PLC06_00245"
     CDS             complement(1572..2363)
                     /locus_tag="PLC06_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88906.1"
                     /translation="MSDEFALEFTTQELKFLGSLFRSGDEARDWGDENTSSGPAQASL
                     AVKNYIRPATNVEELVLDTSVAALISVMGDPQSAVLVSSFHGRAVEPEQLRLCAARGL
                     IVDQRDDDNNHTLTAIRTRDVALQRLLAFVGLGKQPAAHTESFRLATDEMAKLPYVIA
                     GGGAQDGESFLREAGAPAQAAARLAAALDSPVRQSNVRAAVWRGGEPREVGRLTLLEE
                     VYGLWLIASVEGDQVAIIPVAADVAAGHIRELAFRVLPLGADSEV"
     gene            complement(2390..3208)
                     /locus_tag="PLC06_00250"
     CDS             complement(2390..3208)
                     /locus_tag="PLC06_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88907.1"
                     /translation="MQTKDETLELRLSLEEVVVLLNLMGHAQLAGETLRALVGPIAPD
                     EMRGRLLAANRSLLARGIFKLAGDKLQIDPAYARLLMPLADNRFVLRCAAREPGQPEE
                     AVTFYVRDDVVVEQRIAYDVAHTLREVADVAAASAICEAFLDLARPIPFQAAPFSLTD
                     EQLEEARRLVAVEIEQAGVYLRDLGLDDDTNAFFVDDLAHQRRRGSAVRLEIDAVAET
                     VRDSGFLSLVGESGRGWLFIAAAAATGPIVQIRPADSNLVRQSLEAAFAPRMTV"
     gene            complement(3254..4957)
                     /locus_tag="PLC06_00255"
     CDS             complement(3254..4957)
                     /locus_tag="PLC06_00255"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRO88908.1"
                     /translation="MAISSARYTDLYDYARTAEQIDYDLMGQADRLANRLNYFEATCR
                     EGGFQVSSDSLGPAMRSYSVRALPVDQHVRKVGEDFQRADGAWGSSLWLPGWLPGWWH
                     EATSWAERFGDWWAWLGGLAFAPAYLVMTMPHGTKYMHEIIIRGPALAREILGLSPNL
                     GHIKLANLTSHLWKNALNVGPWFLVGAAIASAEQWMKDIESYQGDLVGMINALMIDTE
                     VIVATMGLGWVAATGISYVIFHGVVIAGVGIGGVAVGSTLAPFLVTGGIVLGIGLLAD
                     KFLLEPFMKSEFRLDAIEWLTDKEYDLVNFLDARVDEFLGRDMQPMPVVGPIPMPRPT
                     PSRPPSPVPPARPVEGLGKIEPSPFAELVQKAKIARAEDVPLSVSRNVNTPNPPENFT
                     PYYRGSSDTSKSNCTWYAATALATYTGGKIDLHGVHGDYRMGDAGNWPVQAMNAKENV
                     DHPLHGFVTDVDKKPAAGTVITFDPKKGGAGDVGHVAWVEEANVVMGKDNKKYWELVI
                     SEENYGGGNWKGATKITTGDETVKRWRRTVTYPVSENDGSEAVTDGIRFIHWDPGYKP
                     E"
     gene            complement(5054..5335)
                     /locus_tag="PLC06_00260"
     CDS             complement(5054..5335)
                     /locus_tag="PLC06_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HRO88909.1"
                     /translation="MTGRVLSTDIARQSITTMKSLIDGGLAEQIASLHREGTTLSDPN
                     NWDGQLAVQFRGDWTQMNTTLNQLKEQLAQLQAQISTINQNIMSAGGNA"
     gene            complement(5469..5858)
                     /locus_tag="PLC06_00265"
     CDS             complement(5469..5858)
                     /locus_tag="PLC06_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88910.1"
                     /translation="MPNWQPNRSDVVWDWGAANDAANELERAAGLLDELTARRRRAAD
                     TAREEWRGRYRDEFDRDLDEMLRRAGELADQMRRKAREIRGAADRARDQQNFRVSERN
                     RWESERREEERREREERERREREQRNR"
     gene            complement(5899..9921)
                     /locus_tag="PLC06_00270"
     CDS             complement(5899..9921)
                     /locus_tag="PLC06_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRO88911.1"
                     /translation="MTESMTHPPKFSQGPRDFCVPPEATLKIPSPGPHPGEPKLRLES
                     VLIPLGVMITGAILAVALLNSGPFVLVGVLMALGGAIVTFTGYWRNKSEFRQSVALRK
                     ERFERILKEREGEAVALRARTQACLMKNDPGIDECLQLAESRDPTRLWARIANDRDFL
                     RVRLGLGAVPFQVKIEPPQQMNPLVRDPFETQAEQLAKRFETVTDVPVLLDLQEAGVC
                     GVAGERSDAANATRAIAMQLAAHHSPEILRLAAVYPSGEAGQWDWMRWLPHVWRPDRQ
                     LRYLADDHAAAQVMLDDLAGILRQRDNQLQARKNATGAPGWPVYYVVFVAATTALNPE
                     TRRLLTERGPALGFHAVFLATREAELPRECGAVVTVGAAPVLRDRHGQAAPIPYAADE
                     VGVGAADKFARLLAPLRFESSAAAIPNLVSLFDLLGLNRVEEWRVIDQWRRNDSTQSL
                     QIPIGIGAGGRRVMFDIHDDGHGPHGLAAGTSGSGKTRFLECLVATLAAHYHPHELAF
                     MLVDFKGSDFLQDLKYLPHCISILSSIEGESEEQQSWHATRALKALQAESKRRQRLFA
                     DVGVGKISQYHRKQRAAPDRLRPVPRLIIIIDEFAELASQQPDFLAGLVSLSRIGRSL
                     GMHLILSTQQPSGIVTDQIQANSRFRLSMKFTKTEDSQAVLKRPDAAYIEQRGRGYLQ
                     VGENEVFDLFQGPYGGIPYEETDPALIDKERELEVIQVALNGERVLHRKQEKPRPART
                     QLTALIDHIRHEMAQAGIVPVPDLLPEDTGGLVALDDIRGPGGWDGRGWARSDAWLQP
                     VMGMLDDPTGQLARRLGGLPRLRPDLERFGHLFICCDMAEHTQLPLRTLVTSLAMDHS
                     PAELVFYALDFGNNTMGVFKDLPHLGAIVRVTETRRITRLFRWLFTELEQRRELLAAH
                     GLTWAQARRQGIDLGRPAIVLVVDNLIKWKDEADRRDELGALINEGARNGIHLILAGE
                     ARSAVVFDSILGSIGPRLALGFPDRRAFREIIDSMPHDQNVLGGVPEQGVYYDVDTGP
                     IECRVVAPVRADSPDEHEKTLRALAREMKDAAGKARLPRPFEIGELPPKLSLAELMPG
                     DIGRAWRDWHTAPHLRAPIGLDDLALGPLSADLREDGPHFLIAGPPKGGKTVALRTWL
                     LSLAARVPPEGVRLVLFDNFHHTLDPLRPLPHVWRVVSSNEEIKELFGELRELLAGDT
                     AQMLDPPVVLVFDDLSQFNDLPLRTALAGLVTQGALRGLHVLAAGRTADMSRYAELEK
                     ALLKYRSGLFVGSQTVETETAFFDIALPVGLGREKLPTGRGYLVRQGDYHMLQVATPG
                     DSDALDDYVARIAAAAMQAA"
     gene            complement(9940..10428)
                     /locus_tag="PLC06_00275"
     CDS             complement(9940..10428)
                     /locus_tag="PLC06_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HRO88912.1"
                     /translation="MQSVIVSVKLKGQAVEYDLEVPAEVPSRKLAELITRNINPQAAV
                     LANLTLRCRGPGPGRLLPLDMSLAQAGLWDGAQLEIGSQNDADNLSWHELIFGWVPIY
                     EESQPIPRMIPAPPAPRPAPLPRPDKPMMDDGEVEWIPLTAAPRPNNPTPDEADDGWK
                     PA"
     gene            complement(10432..11775)
                     /locus_tag="PLC06_00280"
     CDS             complement(10432..11775)
                     /locus_tag="PLC06_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRO88913.1"
                     /translation="MSQESSFRLDQKRESAPQAGENAFLGYRYAYARAADSRRDDEPG
                     QDYLAFRDGADHFVFAVCDGVGQSFYGDLAARLLGDALVDWLETGPPVADHPAALVDS
                     LPAFLSALTATAAERVDQYPIPDGPPLLREVLLEKQARGSEAMFVCGRVDAPGPARPN
                     GRVLLAWMGDMRVYLRRGDRPVDLGGVHETDQRWSSRRGPVGGAVNLYHGPLTDAQGE
                     PARLVAYSDGLKVLDKWANLRLTDVGLQRAIDHSNSLPDSDDLVYLELTTHSAQPATQ
                     PRPAAPAQQAPSTRRPMAPLPRPEPQPVPPPAAPVPAIEQPARAAAVVRRAPRWPIVA
                     GGLGLLVLCGILWLVLRPTIQGRLVPILSGLLGLNEAAPTPTPTAASTLVPTAISPIA
                     PTSVPTAIPPVAPTSVPTAIPPVAPPATPASVPTIAPTVTPPGVDVQAASNISMR"
     gene            complement(11802..12545)
                     /locus_tag="PLC06_00285"
     CDS             complement(11802..12545)
                     /locus_tag="PLC06_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRO88914.1"
                     /translation="MSYELKATSRTPALAVFLIDVSASMGQPLGDKTRIDVVIDALQG
                     IFRRMIYLSTRGSNIQPRYHIAIYAYNDQLFIPFDGIKSIDQVANIGVPKMKPRATTN
                     TALAFAKVEELLKREIHKYNNCPAPIVCHLTDGEHTGDDPEPIARRIMQMSVPDGPVL
                     VENIFMSDTLLPTPIVDVTQWPGILPGTRLETDAGRKLRAMSSALPESYREMMIEMGY
                     HIHPSALMLLPGTNPDLVRMAFAMATTTR"
     gene            12905..13063
                     /locus_tag="PLC06_00290"
     CDS             12905..13063
                     /locus_tag="PLC06_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88915.1"
                     /translation="MQPDNAVVMADLRAFAFVLARNLLTGLLSLMVISLQYQLEQGLL
                     IVLRRILP"
     gene            13060..13992
                     /locus_tag="PLC06_00295"
     CDS             13060..13992
                     /locus_tag="PLC06_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO88916.1"
                     /translation="MSVCPNCQETNSDNSRFCQFCGMPLPKKQVEALSTTIPLTQSTS
                     IPHQKASTQQLDHNIGQLGDGGTAGGSLWGPFAGFGTRGRHSSWLLDNLGEKAGALHE
                     AVTQRFQQRQIPNTSMKWYALVAQGVLVERRPFYFVRRGITTVALYIAQFGNDLYISQ
                     VTYAKGPISNVRIGILALMALFQLFFMFQFTNTVVGQMESYVSSFNPFGGGSSGSTSL
                     LAIMLCCVGPLGLLNTLAWLFILAFSGFKYLTEKDLPAILRRPPNEFQIDDTIALEKA
                     VEETVRQSLDAIGIDASLMIPVQDPDAGRTIRLI"
     gene            13996..16704
                     /locus_tag="PLC06_00300"
     CDS             13996..16704
                     /locus_tag="PLC06_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRO88917.1"
                     /translation="MVNLIGPGHSRITQSAGSQLATAIFGYRIPHLPDFLADHTLLAE
                     ESQEQLREVLERLVRFVAGLRKWRNSAFSLRFISDPSKGRIDVAVLGRISGHTNTIRD
                     MTAQVIIDLGINLALYGINVELLDVENTGSSGLNYYLYPFSGTNTIVEVRQQGVVVPM
                     MTVQKEAYVVYPFWGPSTPFLEPFEALMRVAAPVAISIHLEPTELTAAESEALFTAAF
                     LADTVADMDRRTFSDASARRLRDPAAELVGRIYTAYRKTLDEPFLLLSQVSGPHPQTT
                     WTIARALATSISTGNERHAAEAAERGLPSQAAIVAPGNDAESAAARRTFTSLYLSPWG
                     VTIASPGKERFPYLVGAEGAAAAFRFPVSVRGGVPGIMVRQRPPDFEIGPRRQEIAAD
                     EIHLGNFHSGGAVTVKRNSLLRHGLITGFTGSGKTNTMLYLLDQLWKQHRVPFLVIES
                     AKKEYRGLQNVPGFEKLLVFTLGDETTTPFRLNPFELLDGVRVEAHLGRLQTCFDGAL
                     PQFGILPSIVAESLELIYREKGWRLTDRGGESNRPFPTMRDMFRVAVRVSESRGYAGE
                     TYQNIRAAVGGRIGGLLRGSKGRMFGAQRSYPLDVLMSQPCVLELNDLNADDKSMTSM
                     FLLMLLREFREQHPGHTLQHVTVVEEAHNVMGNVQSTGPSEVAADTKGQAVQSFADML
                     TEIRALGEGVLIADQSPSKLMPDAMRNTNLQIAHQLRDAKDREAIGRAMIMDKAQQDY
                     VGKLSIGEAAVFLTGMEKATFMQVPPYKDDAGLHQLPDDAAVRAHMQSFIAKNLTAHL
                     PFDGCRFCGSPCEFVELIEPQTREPELHEQFIGALGKFEEAESDNELAASWRGVARVC
                     YDAAIRGARPHPDAAYCYLAHEIDFPFTEHMRREFIRAYESVTRLG"
     gene            16767..17339
                     /locus_tag="PLC06_00305"
     CDS             16767..17339
                     /locus_tag="PLC06_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRO88918.1"
                     /translation="MPVAQPEQSGKADADMARIEAAMKGMREQQDRELKEDVAAFDNR
                     PKPAYYQDSAPPAADVREGAHWEDRGIIDVPVADLPQPEGVDGPQDFNHHIKYEDALS
                     ATRTVQEMRPQIDAGYTSDDFADLDKAKGLNYEHGQQRVYDLYYGSDPIAVDKDGDKY
                     DIVSGRHRIFAAKEIGLDFIPARVKEKVPG"
     gene            17367..17933
                     /locus_tag="PLC06_00310"
     CDS             17367..17933
                     /locus_tag="PLC06_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88919.1"
                     /translation="MSPPKNESTDILVDLKHEGTKSVDAGLSEKGIRTMQGCGTERVS
                     AGRKGSDFEQWAKTNVFHGEGHRLVALPEDNEHLDKYGDGLGLTKGRRSDSYIMERGE
                     IWELKSGYEKGGIDQQQLLEYSIMEDAGHVNIRTAEGQLKVMPVKSINYLFETKDGAA
                     ANMNSLRGQATAWYKNNEGQVHLYTEDA"
     gene            18002..18331
                     /locus_tag="PLC06_00315"
     CDS             18002..18331
                     /locus_tag="PLC06_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88920.1"
                     /translation="MSETERYTPPEMRQEASPEKTSSLRPETISKIQGDSPNHAPITP
                     ERQELHKGRESLPEKEPGSPFKPSGDPSAFKSDSSMTDTQAPVSDDPSASTNDDAGTD
                     DQASDVQ"
     gene            18360..18530
                     /locus_tag="PLC06_00320"
     CDS             18360..18530
                     /locus_tag="PLC06_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88921.1"
                     /translation="MPAEMILKETTDAARKSVKEGVNMIKKAWSIMDAASEKKTPQEV
                     LSPKAIGKIQGQ"
     gene            18712..18858
                     /locus_tag="PLC06_00325"
     CDS             18712..18858
                     /locus_tag="PLC06_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88922.1"
                     /translation="MNSSSRLVRTICFVFGLTLVVGSIGCIRGGDQVAIEVAVAQTIA
                     AQAG"
     gene            18907..19608
                     /locus_tag="PLC06_00330"
     CDS             18907..19608
                     /locus_tag="PLC06_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88923.1"
                     /translation="MAPTDTVAPTDTPAPTETPAPTETPPPTVTPTPTETPAPTDTPT
                     PTVTPTPLPLPDFADNFDAGLRPEWKLETDDAWASVNGRLTSLRHGYPMVVGDESWTD
                     YTLSLDVIQLMDRCSLSIITDNNDDGRVDKELKMEMNPTNNIYWLIPGTTDVMPGTYI
                     SDIGSPFNLKLEVKSDGAVTTLINDEVVTNIVMEGYTQGRISLDCHRPGRDGSRWFRP
                     GSSFDNLQISPLTVP"
     gene            complement(19656..20321)
                     /locus_tag="PLC06_00335"
     CDS             complement(19656..20321)
                     /locus_tag="PLC06_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO88924.1"
                     /translation="MAIGGLPIAIAILSNRQIEIDGIHYRLAGEHDICISGVARCGAA
                     LPQLLFRGGADLLLADQATPTAPDDATPYPILQCLPDLRRAYPGLKIALVAARGESSD
                     ARRARREGAVGLLLKSDHAAWSNLAQVVRQLALGDHYVSPSLGDPWAERESGRAGLTP
                     RQFDLLSLLADCPDMTTYEAALRLDIAHSTVRNMLSQSYARLGVSTRAAAIARLRGDA
                     GQS"
     gene            20791..20967
                     /locus_tag="PLC06_00340"
                     /pseudo
     CDS             20791..20967
                     /locus_tag="PLC06_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008108598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            21158..24013
                     /locus_tag="PLC06_00345"
     CDS             21158..24013
                     /locus_tag="PLC06_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO88925.1"
                     /translation="MVRVQADDLPAQTSSAACHNLDLVVLVDQSASMRNNDVNRQRIE
                     VPKLIVEQLGNHAILLCPDQNIVHRIAIFGFGDASRYLGPENNYINDTSEYLSPQSIP
                     LSYEYDDWVRSRDALKQQLNAFRNDNLSYTDHKSALLRARDVLQMWKQQPVAGAEDRR
                     QAILLITDGEPCTGSGGCSTNPGQYKFDRVTYMNDLIQVAQPSSVDFPYVDGDPNNTI
                     FISMVALSDSSQSYNYRTQPTFRNGWQTIIGQRGQIYDTQESNLDLATGMFEILRPLI
                     GSNLQEWDCRRPINVVPYLDSSLVININRQPTDSTVDPASIAVFLDVQTGGQTITLEG
                     GEQVWADGTRQPLPPTIKYTADGGDDGTVINENYAFTNPWPGVYNVRTQGGDVCHDIR
                     VNYGKGSVTANILHPGADTVLVQAPQEPYYDELAPNYLSLAVLDEGAPLQEIDGFPLH
                     IHAEIASPQDNQDLGELTLSNKGIYDASDPILLRDASDYTWTAIGCVESPTELVLLSA
                     QGYDGQIVSCSWAGADDNLIQVFSASGNFSVFPVSFFTWRITNPLDGAMSAMNSVQGS
                     QQVPAAIPVVVELIGEDGNLLPASSVFNKPDEALRAELYKVGNDTPVESITLSPTDAA
                     AAEFSGQFANSAQAGAGDYRIVVRPNWEPTSIDRTKFAVQNNFIAAEAAHSQFEVYPL
                     DVRITPPSDVALHSTTIPQCFLKPGECFGNAVQPFDFNVELVNMKTGLVVPLADALVD
                     VNAGHTLKVAAPSGAEEIIKLKPLNAANLQMLQAIGAGTDLDEAGEYTIEVSLAGIPL
                     RDGFSWVDSRRTATFSREDTLMTNPLLAKGAVILAGLVLLALLIWLIYIHTGGPSGPL
                     LYDSVNSLDQTEIVGEWKLSSRKRTNVLKSKTLKERGVKLIKVTKGQALMKEGAKAIQ
                     IEAEDLQGLPLYSGPMEDGDIQSFNDGRLHYGGPIIN"
     gene            24034..27708
                     /locus_tag="PLC06_00350"
     CDS             24034..27708
                     /locus_tag="PLC06_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HRO88926.1"
                     /translation="MKQKHVESNDSSRYKSVTEEINIMTRPALIVGLGGTGQWVLTWL
                     KRDLMLSNGGTVPDNIRLLSIDTATTLEAGQKQVTANDKEDNQPAKVGGVTLDRTEFI
                     YVGGDARPIALETRDNRHPQLKRWFRAKYWLDTQPPAVFVLDNGAGRIRQFGRLAIYK
                     DIMGGDDGQKLWSAFRTAIGTVGRMTNAERRLEIIVVGSFAGGTGSGMFLDVGLILRK
                     LAKEISPHHVLRGFFALPGVFTPNPSRDMKARTFAAWRELNRFMVIDSDFPMSSVDYV
                     ENDSTYGIDPAERIFDACYLVEGKRKGAPVASEARFGVFPMVSEVISAILDEQAGQVY
                     NDWVYTNLAEEYGKHPTTPMYSAIGAYTVQVPANFVEVMSSHIFGQEMLKRLLSPEVA
                     PDANTKKLVASGALRHLTLAAKARNLEDPGFAGRQRADTFLTSESAYEGQMVNPTIFM
                     ARAATLLSEAREQGKLPQVIDRLARAGTMGGGGAEGWARFYPEFGNDPEFESLLRDVR
                     DHTHFNIVTAFSRREKEKAEDFRKRIIQLDPLVQTKFGSSKIGGDQMIEYDGSFGVVL
                     DRVHETQMIVFRQALRLRLLSLLMGRDANPLKARGGKLGYTWDFFDGVAANLDWFLNQ
                     VMDGVSKRRKELRPSIDAEIRNRKAEDYMDKVRDKRFLFLWESPSVKKSETLFLRTEQ
                     EKMDIRREDILYRYVDRTLRAMKAAAEQTRDAVQAWIWHLSTGDDASELPGIWDEMLS
                     SEANVKEAHGWDQSTDTVQRMVAEEVLPVSEEDLANILRRWQWDAGYEHEHLWLKVNI
                     LPEAEGEPIDVLNNPVRESLARRDEVGAHNTSRLLGLARRNYAGLVGRTTVAETIIDE
                     YGPNGGVRFANDVATKNAEPLFVGSTTNKPKRQSNLIRVKSPANDQFFTGKDSAQSQL
                     RVLAQKDPVNADANYLIQVVGSENPFKLTLVRSDDLYNFDEFAAWEDCQSAYAAHVND
                     KEGLMRPVNLHNFAAEANAVEFETRLVKDKKKSYRPLHPRVVMLLENPEPLRQFLYLL
                     ILGNITEAHDRSAYHWELSWDRADGTETIYLTKPWNEDEERAHSAKPDIFNAAHGYVI
                     RQKSHEKYSQAEVDIEFGRIYIDRAMADAGRQAVHDVLNNELNGKGEIGKMERAARER
                     DKNTIIRQDLEDLAIVFRMMLQDDLSHHQPVIEERRENAFERARRLREETEAAAAAEA
                     TKAAKARSKTNKTGGSTKTT"
     gene            27869..31693
                     /locus_tag="PLC06_00355"
     CDS             27869..31693
                     /locus_tag="PLC06_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO88927.1"
                     /translation="MAKIILGAWLAVTLILAVPIVITLIRGSVSTDSFSRSAEPRILF
                     GPGPSHVQVDFTPESTGCPVIPLSILLLIDKSGSMNDGNAFPLAREAAIAFINNVDLS
                     QSTVAIAYFDDTAHLIQAATQDRDALITAVNQVTLPYDGTDLTRALQFAASNIGSDRP
                     VVVLLTDGGANDPAGAMAAGDALKSAGAHLVTVAVGPLAEVDYLRSLASSPAAALASD
                     DPADLQSIYTSLAGQLNNIVARDIMLTEPVSEGLTIVPESVAPLATQNGNDLVWQVGF
                     IPATGVTFRYDVTTEGIGWPKIASETATMSFIDCQAGLASLSLGGGPRILILPGPAFW
                     IPWLLALLVPVFLLFRGKKQVPPPVTVAAASSVPIASPDPTPAWLRRLDSTDKVLGIS
                     EVESESGTLVPTIIVGLGPVGRIVLSQVAQALNGRFARRDKLPVRLLQVDVQPTSGPI
                     SERPDYLLPDEWVILRPDYQLISRTLQTHPDEWEHMNWYEPTAAADYGRARGRMALFY
                     DLRNGTANSTLYRGLMKAADNMVNPRLRVIGSTFDDVSSGMLVDVSWLMWLATKRHVD
                     VELWLSGPLNQNWSPRLDNANHTVTVDEQRARTLATLREIERFQRNAIVPLHYVPHDM
                     GQEEFYQEVDSAVVQTLFLFEAPQDKKIVVEDHLATLTDSLVALLNPSVQKEVTEHLV
                     RFQADAFDLVNRDGHGLVCGIGTYAISTPLAPLHEALTWRLVHDLVCEARTGLLPARQ
                     LGLDGKYETKKPDDIISFTVRRTEIEDFIDNRRAHLHHDAFRQAVAAQVADMLNGEGG
                     KIPAIDRSGGIRRARKWVDMLRTVLKQDGEEMAAAPLADLIRELDEWDNFLHKDLSTL
                     ATERLAAARERLGKLTSQGGRSWSIDAELEWPAYKAHIRSWLDRPSTALAGEAIVRVC
                     QRFGWQVTYDPTAPGWQVELWIPDGEFIWAGQPIASGGLAVPQDSAKVLDHLYHLARP
                     LAYNTESHGNVLKRAADMDSVTWLNAAEPRLRVNDLTISKLLKGANSVHPFLAARETD
                     NTPKIRQGLSSTGDAQQVTACAIEDATTVTLLRVRDRLPMSATYLYGEDRDGNSAWNQ
                     AIVTPAHYVWRGEQAAAEHEIGEHLGSRFVGYLERDEKLVDLYARAVLFGLFEETRDG
                     VAIGGAQGWAWPGKTAGDALVNLFNVDSTQRPGVLNERGGAGRMEAFQAVEEAIERSV
                     EHIRSSTGLAQFRREAERVLQKRQRSRIELESVEPWQRDYEDYFRPLAKDASSEDRDL
                     RLYLLYILSTLQ"
     gene            31732..35394
                     /locus_tag="PLC06_00360"
     CDS             31732..35394
                     /locus_tag="PLC06_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88928.1"
                     /translation="MKSTNDQYRIIKYLGEGLTAIVYEAERLTANANGLNFPIGSHVA
                     VKVLQEGLPDDIRRNFRDEADVIAKLSHEETKAGLKRSLIPNLVEQYLGSGAGPQFLA
                     MEFVQGTPLDRRVREGDPLSEQMGLQVADSVLTVLDLLHTQLHKSYTDFQLQNVWLLP
                     DQQNIKIMDWNHVSREQNPLPYELVDADLRRMGAYLYQILTGKGAIQDGETAEALSRR
                     AGKHWDNLSVGTRHLIRRSLNPNPANRFRSAAEFRDRVLQQHRLWEANTDSVVDEAQT
                     LMRPVTKAIADGSRLSTDELTSAETWVDMADRRHRGDQLVQYWKETISKATRDVSATW
                     GSGMQLFGGKQYREAAAILRNEASSWGRADLWRQVMTADAAVELGDGLFQSVAAELIK
                     VVDTMNSGNWEEARELFGAPPLAAAHGVAIGSLRGEIDAQIAYQAARPAFNEGRWNAA
                     ARDFHIVNEALPKIAYAEVLRTQYGWDGVDELAAEAKRLAEDVERGNAQIRELTDALS
                     RDLDDGLAQIQDALLRRSDSPEIIRLCEREAERRPASEAQRLLLTALTYGAVPAAQEV
                     ALKSAYKENEERIKKERLQAEAAQREAAEARTREEINSQIQAGRWAETAGLLGAIPLR
                     LSDNVIDQVRSAFDAAVDGENSRQAREIAAVLDVVEPGNADVRRGRLEAQQTRQKAAT
                     DAWQTNLPNQVQGIIAAGQFDEARERIHLALPLLPEKSPITSFLRKQEQQIDEFTAAQ
                     TKLDDIHDKFSNRGLGPDDADKALNLLDGRIAALGASLTPAATQHLKDSADALRYDVR
                     LRRLRNELDEAVKAISAKSYDTAESKLADAETVLNSVPSGHPGFSEQQERLTNSKSRL
                     ERERSAGKKFWDRFGKWIFGALVALALVLAAGAAYGLVSANRERDVRATTESALATTD
                     ANYNFVFAELEKLRQPNINVTQTVEAFQTRAATDAAALIVANAAATALVASNEGLSGT
                     VTAAQTTPEPLNLVTNLVGAAPVTGTIGEDVPVFYDLPISMNVTAPPDWSFQIDPERG
                     LQLVDPGETAHTLRLVHTSLSRGTTIQTAVPASSVQLNPDTPHEATIALAALPVVLPN
                     DSGHYRLGWEAVAADDANNRRVGASVAEFEIISPPTVMIKSERLYRTRPLWEDSFIVP
                     GTGDLTVEVLGKLDLADEQTGDGQTPPVPDFLMIRLRGSREIFWLPSWNAVEFDDSEK
                     WQALLDSLPEVSAPDA"
     gene            35441..39856
                     /locus_tag="PLC06_00365"
     CDS             35441..39856
                     /locus_tag="PLC06_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88929.1"
                     /translation="MTHEEKESLINDLRLWMEGVDDDETYGRMGAALRRIRAWDPSGN
                     DPIPYKSEIEQWANARGGASAWPPYSETRPMPDDTQSKEDEPLSTIASAESAESADPD
                     DHLQEAYDSAMLRMEGGAYYEAMQGFQDIAEKARGRLLRPAEEYQFRAKAELEHRKAP
                     LLDIARAAAPEAAEAAWEAVRGVDPDDVEAAAALETSQSQAVVERTRRRAEEVLDEAN
                     SAFNRRNLGALNLLVGEVDTLRRANKAIALRPVLDDLLQDVIQRRDQLRAQLGSPSTL
                     IATGNNREAYARARQMFVEKILTIVDTAGIMGGEPGAEVETARFLDKAREIYLAGLNK
                     FAGDRLTVAESEQGVDPSGALETLNEVVSALTDDILTGDDRVHLQNSLDTIEKRIKSV
                     EMRLRLYEQAQALVVAAGEPGLAADDKFLKLRQAREIYTEYPEIDDKLRAALTDVSAA
                     AAADLEGEIALAKAQIKRDDFNEARRILQEARVKATNKVPDPIPGSSLHQRLDELSKL
                     DDEISDAEVAYERLMSRLAGINNLLNDEDVSRQKANLIEARSRLESLSPSDTNREEVV
                     TLKGRLRRLQGLDSNWTEGQRAYQAGQWTKAIDELALVAAAANHTARDRADVMAKRAE
                     AAQQVEVAVEAEKERRFGDAYAAYTDALTRFKQYDHDPQTQGTRRTAEAAIERLKPYT
                     DGDVEVGKLRRDAAARLAQAKAITDSQRGSPSLLQPNQGYRDAIVKLGQAAKVEYTNL
                     GPQVEADLQKVRAQWEEANSEGMLLAVGSDDPEVLEKGITLGQELENEKLLTKPTNRD
                     LLRRLRLKALDIKYRRLLSNRSTSPAEIETNRRDRLNHTPDDAGDRSEIEKQLSEAVA
                     NRVIVEMGQKYDSGGADAAMKYLSQEMNQPEVYGSRALFQEFMQLLWEEEHWDDARTE
                     ADRLRFRQLARPTELRQLWHDLTDIAERMCANDKKAFEAQLDSLNEWFLAGSDHSGIG
                     GAGGRTAYVWFTDQNERRAYINDEIAQLKRKRVDKLVRQAITEQGTGNLLKVAQYYGY
                     ARLWNPGDPRVINGLQGVGPQLQNSLQALLTRAGNVTLQANAFAKSLEDADEIRKQLV
                     DIREVGNALGLTTTTMNQLSITAESLKERTDGWRQVEMILKGIDVDARKALRNPAGIQ
                     NSQGGWDLTNVLTQFSNAVTEANKQATDRTALNTIINNHRTDLNNLNSDADVLNKDVV
                     AFLEAVDQEDFDKVISLGKSLDTTWSARQSSGFDGLEGLITRKMFYGSGELRTLKAHI
                     TTAQNQSRDLARWQTWADKTVAAHDVLDTAVSQLNKPMDDLRDTYSLKELKEMCEAAL
                     NLVEQFLNVAGQKPESNPLSKKAQEQRTRIPDDWEEDLIAGGPENPRSIILQRKGEIM
                     DDIQKLSGQNGPLQQLNGAMTQLTNAHKEAQGKNGGLFGRKPKGVSDNLFKNAARFLE
                     ACRKLDPANPDVRDARKGLHQLATLYNKQDLLKEK"
     gene            39859..40860
                     /locus_tag="PLC06_00370"
     CDS             39859..40860
                     /locus_tag="PLC06_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRO88930.1"
                     /translation="MSSRFALVTDIHANWHALRAIIDDVADVNSSCIDNPYRFWFLGD
                     IIGYGPHPVECLNWLRYKSRIGDRWVPGNHDAWLINPEGVNPDADESLKRHRDLLAED
                     TQSELGRWFREELYRALGTGFGIDPNNEEPRWRVCEKSSDWTLFFVHANLFDRRRTYL
                     WAWDPGQLSLEFGRMKKEVGEDANAVLFCGHAHYPLFARQLPNPDESVALQSIRYFTR
                     LKIQPGLTIINPGSAGQPRDGDSRAAYALFDAEDMTIEFRRVGYDVDKTIADLDEKKY
                     PKSLGERLATGNGKSELASYFSVYRRPRWDLEAYESSAPGELPEEPDEHWYGRRGDA"
     gene            40826..41392
                     /locus_tag="PLC06_00375"
     CDS             40826..41392
                     /locus_tag="PLC06_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88931.1"
                     /translation="MSIGTGEEVMPRKNDPFADLDNMSWDADFASHDSRRQKIFLIVV
                     GLVIIGAIMTGIYYLLSGTLFDSPERAAERWLNALAKGDTDRMLELTCNDQLQTASIL
                     SLPEMVGGDALQELGLSEWLKNIDFDMGNLKYTGSEVSAGESEVMVRGILRIRAINDF
                     WLPIPLNQRWRMIEEDNAWKWCGYIPQA"
     gene            41404..41604
                     /locus_tag="PLC06_00380"
     CDS             41404..41604
                     /locus_tag="PLC06_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88932.1"
                     /translation="MNRIFAFFLFIIGAGLVAIGACGNLTYLVSLNTWGIIWRIGLGV
                     LLIILAIILANADSITGSDDPF"
     gene            41619..41762
                     /locus_tag="PLC06_00385"
     CDS             41619..41762
                     /locus_tag="PLC06_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88933.1"
                     /translation="MGLDIFTQMLIVFAVIVLEQVIVWFAEDFWEFRKQISQRGIGKR
                     GGS"
     gene            41759..42772
                     /locus_tag="PLC06_00390"
     CDS             41759..42772
                     /locus_tag="PLC06_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88934.1"
                     /translation="MSSDEWELLSPRYFSDILVPLFNGGLIAYAVIFVLLFFGTIIMP
                     IIVKLIRKQATQRYANNSSQVDPNHKKTLLTLTDIPQYLPDDRWSYRLFYYTSLSLGV
                     LYFSFHIAISVWPTTILTTGGKLQLLTADDIEAMIQFLYRWILTGYALVVFSISLGLD
                     FVARWMAVSWPKEKQPDTGPQEPAAHPPSPPTPASSTSLPMGQTSLKNVITSIKQSSF
                     LVEIRIEKSNHRKKGEDKLMHDSSRAVCVSTKGQIEYEDEWPTGDEMQRLLRFRSNDG
                     VWCVAYVESPLVGTSVKYLKGGRGRWQLLTNEMPINNEIIKFQWGIDGVRYYCECTRY
                     VKL"
     gene            42873..43931
                     /locus_tag="PLC06_00395"
     CDS             42873..43931
                     /locus_tag="PLC06_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88935.1"
                     /translation="MGENGAVVNHEGFPATEARFLILDDGSPIHTAWPEDKILVLEDD
                     QKVSAEYDPYKQPIDTTVVYHYTEYTDIESGGSHREVMDELVANLKQETRLRSLCLTM
                     VSVREPVPCRPPGTGETLGESFKSRTDYSRTEVTNSIRPISSQLIWDSIIAERVPNRP
                     HLVIWVVDDDILTHDDMPGDLLPATSDQLTRLRDADISLIVLDLTPADDLLRDEQWKG
                     MLKTFNILHLKYAGTNYLLNDTKCGPAWICVKQELDPKRPMVHVLRYNSSLFVDGAQH
                     SLRVQLQSGNLGGNAGTASSIIAHERTDFIIAPPYQNDHISLRSEVRFFSRAFNAIAM
                     IVLMLVLVLIKRPQDGSV"
     gene            44387..44746
                     /locus_tag="PLC06_00400"
     CDS             44387..44746
                     /locus_tag="PLC06_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88936.1"
                     /translation="MQDVQDISSPTNGFEYSDDRTYIELHGSAAWPADREGQFLKYEV
                     FYYDPGNQKHDLAEVWTSVSGGLLHRQAFGPLVHQYGEPGWYTFGVLGIRTDSNHFPL
                     GSYNEGCFVRVYLPWPR"
     gene            44871..45395
                     /locus_tag="PLC06_00405"
     CDS             44871..45395
                     /locus_tag="PLC06_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88937.1"
                     /translation="MEKLHAPVPGEVWLLHTPPRQAMFALVARMATIGPVLALDAADE
                     FDAHQVARLIRRRAPRRFNRSLGHVRVGQAGTCYQVLALFKQLPATSMPHIIFDLPAT
                     FCDESVPLPESRRLLQIALNHVDRLRREAPIIISVRPTPGGRRAELLQAVIDRADHLF
                     VWESPGVPVPVKLF"
BASE COUNT        10017 a        13175 c        13178 g         9082 t
ORIGIN      
        1 ggccgtcgct cagcccgtcg ccgtcgctgt cggcgttgtt ggggtcggtg ttgtaatttt
       61 gcgtctcctg gctgtcgctc agcccgtcgc cgtcggtatc gctgtcggcg gggggaacgg
      121 ccgtttcagc ccccggcgtg gcggtcggca aggattcggt cgccacggcg gctatcatcg
      181 ttgctgtcgg cgccggctca ctcccgccac cgaaaggcca aaggccggcc agcgcggcgc
      241 cacccccaaa caagatcagc aagagcgcca gggccggcgc agcccatcgc caaccgattc
      301 gcctcggcgg aacaggcttg gggggcggcc ctgccttctg tcgggccaga tccctcgcgg
      361 ccgtccactg atcggtcgcg gggtcgccgg gccagccggt gagaagacga tcatagaaga
      421 cgaccgcctg tccccatcgc ccgttttgtt cggcggccgt ggcattcgcc atcagatccg
      481 tcctggcctt ttggcgcggc gaaggcgggg gcgacggcaa cggcttcgcc tggtcggcgg
      541 agaaggaagg gcattcggcc aacgccgagc tgttccacgc ccgttgcagc aacaccgtag
      601 cgtctgcgcc ccagttctcg gcgaggaccc tgcacagcag ccgataacgc tcgcaatcct
      661 gttgcagttc gtcctcggca aaatagcttt cgccagggat gattttgccg tcgttggttg
      721 tgatgctgtc ggccgccgct cgcacccgct cgtcgcacca gcccaaaatc tcggccagca
      781 ggattgcccc ggcgaagcga tccgtgtcgg cttgccaagg gcgaccggcg atcgttgcgt
      841 gggcatagcc cggcgacccg gccggaacct tggccggtcg ggtcaacccg ggtgcataga
      901 gttgctcgac gtcgaccagc tcgatccgga tgtcgcctgt gtccagcccg tcgatcatca
      961 gattggcgcc ggacaggtcg ccatgggcca caccctcccg gtccatataa gccagaacgg
     1021 ttcgtaaggc ctcggccaag gcctggcttt ggtggcgggt cagcggccgg cgatcctgta
     1081 tgatctggtt ccacggccgg ccctcgatcc agggcatcag cacggcatac gccaactcag
     1141 ggtattgctg cagcagcggc ccttgttgtt cgggcgtcat cacggcccga tggcagacgc
     1201 tgaggccggg caactcgccg aagcgccgca gctggccaac ctgttcaacg agcgaaggat
     1261 gacgaaaacc cggattgaag accttgaaag cggtcaggcc gccggctgaa tcacgcagct
     1321 gatagaccgt gccggcccgc ccttcctggc catagggggt gagcatggcg cccgcggtcg
     1381 ggtgcgcggt gataaaacgg ctgtaaccac ctatgaggat ctcgtcgccg acgttgggtt
     1441 caaacatggt caggtgtcgt cacttggggt gcgttatggt ttgtcatcta taaagcgtta
     1501 atcggtttat cggccgggtc agccggcgac ggccggcgat agcgggtttg aacatagagt
     1561 cgtccctccg atcagacttc cgaatcggcc ccaaggggca aaacgcgaaa agccagctcg
     1621 cggatgtgcc cggcggccac gtcggccgcg acggggatga tagcgacctg atccccctcg
     1681 acggaagcga tgagccacag gccgtagacc tcctccagca gcgtcaggcg gccgacctcc
     1741 cgcggctcgc cgccacgcca gacggcggcc cgaacgttgc tctgccgcac agggctatcg
     1801 agggcagccg ccagccgggc ggcggcctgg gcgggcgcac ccgcctcgcg caggaaggac
     1861 tcgccgtcct gcgcgccgcc gccggcgatg acatagggca atttggccat ctcatcggtg
     1921 gcgaggcgga aggattcggt atgggcggcc ggctgcttcc ccaggccaac gaaggccagc
     1981 agccgctgca gggccacatc gcgggtgcgg atggcggtca gggtgtggtt gttgtcgtcg
     2041 tcgcgctggt cgacgatcag accccgggcg gcacagagcc gcaattgctc cggttcgact
     2101 gctcgcccat ggaatgacga gacaagcacg gccgactgcg ggtcgcccat gacgctaatc
     2161 agggcggcca cgctcgtatc aagaaccagc tcctcgacat ttgtcgcggg gcgaatgtag
     2221 tttttcacag ccaggcttgc ctgtgccggt ccggaactcg tgttctcatc cccccagtcg
     2281 cgcgcttcat ctcccgaccg gaacaatgac cccagaaatt taagctcctg cgtcgtgaac
     2341 tcaagagcga attcgtcact catggcgtgt acctcccgat ctctcccggc taaacggtca
     2401 tgcgcggcgc gaaggctgcc tccagcgatt ggcgaaccag attggaatcg gccggtcgaa
     2461 tctgtacaat cgggccggtg gcggcggccg cggcaatgaa caaccacccc ctgccggact
     2521 cgcccaccag cgacagaaag ccgctatcac ggaccgtctc ggcgacggcg tcgatctcca
     2581 gccgcacggc cgaaccgcgc cggcgttggt gagccagatc atcgacaaag aaagcgttgg
     2641 tgtcgtcatc cagccccagg tctcgcagat ataccccggc ctgctcaatc tcgacggcca
     2701 ccaggcgtcg cgcctcttcc agttgttcat cggtgaggct gaagggcgcg gcctggaatg
     2761 gaattggccg ggccaggtcg agaaacgcct cgcaaatggc cgatgcggcg gcaacgtccg
     2821 ccacctcgcg caaggtgtgg gcaacgtcat aggcgatgcg ctgctcgaca acaacgtcgt
     2881 cgcggacata gaacgtcacg gcttcttccg gttggcccgg ttcgcgggcg gcacagcgca
     2941 agacaaagcg attgtcggcc agcggcatca acagacgagc ataggccggg tcaatctgca
     3001 atttgtcacc ggccaacttg aaaatgccac gggccagcag agatcgattg gcggccagca
     3061 gacggccacg catttcgtcg ggggcaattg gccccaccag ggcgcggagt gtctccccgg
     3121 ccagttgggc atggcccatc agattcaaca gaacaaccac ttcctccagg ctgaggcgta
     3181 attcgagcgt ttcgtctttt gtttgcatcg tcgttctcct cgggtgctac cggcgccgta
     3241 cccggcggct aactcattca ggcttatagc caggatccca gtgaatgaat cgaatgccgt
     3301 ccgtgaccgc ttcgctccca tcattctcac tcaccgggta agtgaccgtt cgtcgccagc
     3361 gcttgacggt ctcatcaccg gttgtgatct ttgtagcccc tttccagtta ccgccgccat
     3421 agttttcctc actaatgacc agctcccaat actttttgtt atccttgccc ataaccacgt
     3481 tggcttcttc gacccaggct acatgtccca catcccccgc gcctcctttc tttggatcaa
     3541 aggtaataac agtcccggcg gcaggcttct tgtccacgtc ggtgacaaaa ccatgcaggg
     3601 ggtgatctac attttccttg gcgttcattg cctgcacggg ccaatttccg gcatccccca
     3661 tgcgatagtc gccatgcaca ccgtgtagat cgatctttcc gccggtataa gtagccagcg
     3721 ccgtggccgc ataccaggtg cagttgctct tcgaagtgtc gctcgatcca cgataatacg
     3781 gagtaaaatt ctccggcggg ttcggcgtgt taacattccg ggagacagac aacggcacat
     3841 cttccgcgcg agctattttt gccttctgga ccaattccgc gaacggagac ggttcgatct
     3901 tgcccaaccc ttcaacgggc ctggcgggcg gcacaggtga tggggggcgg gaaggcgtcg
     3961 gccgcggcat tggaatcggc cccaccaccg gcatcggctg catatcccgc cccaggaatt
     4021 catcgacccg cgcgtcgaga aagttcacca gatcgtactc tttgtcggtc agccattcga
     4081 tggcgtcgag gcgaaactcg ctcttcatga aaggctcgag caagaattta tcggccagca
     4141 gtccgatgcc caagacaata ccacccgtaa ccaggaaagg ggccagagtg ctgccgacag
     4201 ccacaccgcc gatgcctacg ccggcgatga caaccccatg aaagatgaca taggaaatcc
     4261 cggtcgcggc cacccagccc aagcccatcg tcgccacaat gacctcggta tcgatcatca
     4321 aggcgttaat catgccaacc agatcgcctt gatacgactc aatatctttc atccactgct
     4381 cggcgctggc gatcgctgcg ccgacgagaa accagggacc aacatttagc gcgtttttcc
     4441 agaggtggct cgtcaggttg gcgagcttga tgtgacccaa gttcggcgac aatcccagta
     4501 tctcgcgcgc caatgccgga ccgcggatga taatttcatg catgtatttc gtcccgtggg
     4561 gcatggtcat caccaggtag gctggggcaa aagccagccc gcccagccag gcccaccagt
     4621 cgccgaagcg ctcggcccag gacgtggcct cgtgccacca tccgggaagc catccgggaa
     4681 gccacaacga cgacccccac gccccatccg cccgctgaaa atcctcgccc accttgcgca
     4741 cgtgctgatc gaccggcagc gcccgcacac tatacgaccg cattgccggc cccaagctat
     4801 cgctgctgac ctgaaagcct ccttcgcggc aggtggcctc gaagtaattc aagcgattgg
     4861 ccagccgatc ggcctgaccc atcaggtcgt agtcaatctg ctcggccgtg cgagcataat
     4921 cataaagatc ggtatatcgc gctgaactga ttgccatgcc ctaatctccc atttcgatat
     4981 ccgggttgga aaacaaccgg ccgggttggc ggggtcgggt gtcccccgcc ccaccaaccc
     5041 ctatcatcga cgcctaggca ttgccgccgg cagacatgat gttctgattg atcgtgctga
     5101 tctgcgcctg caattgggcc aactgctcct tgagctggtt gagcgtggta ttcatctgcg
     5161 tccagtcgcc gcggaactga acggccagct gcccgtccca gttgttggga tcggacaggg
     5221 tggtgccctc gcgatgcagc gaggcgatct gctcggccag cccgccgtcg ataagcgatt
     5281 tcatcgtggt gatggactgc cgcgctatgt cagtggaaag aactcgacct gtcattttat
     5341 tttcctccag ggtttaagag acaaagatac caatcgatcc tgaccgatca ccagaccggc
     5401 ccaagagcca ccggcgatgc cggcgacgtc attgctcatt agtcgtggat tcatcacagg
     5461 gttgttggtc atcgattgcg ctgttcgcgt tcgcgtcgct cgcgttcttc ccgctcgcgg
     5521 cgctcttctt ccctcctttc cgattcccag cgattgcgtt cgctgacacg gaagttttgt
     5581 tggtcacggg cgcggtcggc ggcgccgcga atttccctgg ccttgcggcg catctggtcg
     5641 gccaattcac cggcgcggcg cagcatctcg tccaggtcgc ggtcgaactc gtcgcggtag
     5701 cggccgcgcc attcctcgcg ggcggtgtcg gccgcccggc gacggcgggc cgtcagctcg
     5761 tcgagcaggc cggccgcccg ctccagctcg ttggctgcgt cgttggccgc gccccaatcc
     5821 cacaccacgt cactgcgatt cggctgccag ttaggcataa ctccatcacc tccatccgtt
     5881 ccgcctggct cgcgccactc acgccgcttg catcgcggcg gcggcaattc gggccacata
     5941 gtcatccagg gcgtcagagt cgcccggcgt ggccacctgc aacatgtggt agtcgccctg
     6001 gcgcaccaga tagccgcgcc cggtcggcaa cttctcgcgc cccagcccca cgggcaatgc
     6061 gatatcgaag aaagcggtct ccgtctcaac tgtctggctg cccacgaaca ggccggagcg
     6121 atacttcagc aacgcctttt ccagctcagc gtaccgcgac atgtcggccg tccggcccgc
     6181 ggccagcaca tgcagcccgc gcaatgcgcc ctgagtcacc agaccggcca gagccgtgcg
     6241 cagcggcagg tcgttgaact ggctgaggtc gtcgaagacg aggacgacgg gcgggtcaag
     6301 catctgcgcc gtatcgcccg ccagaagctc gcgcagttcg ccgaaaagct ccttgatctc
     6361 ctcgtttgaa gacacgacgc gccacacatg aggcagcggc cgcagcggat cgagcgtatg
     6421 gtggaaatta tcgaacagca ccagccggac gccctccggc ggcacgcgag ccgccaacga
     6481 cagcagccag gtcctcagcg ccaccgtctt gccgcccttc ggcgggccgg cgatcagaaa
     6541 gtgggggccg tcttcgcgca agtcggccga gagtggcccc agggccaggt cgtccagccc
     6601 gatgggcgcg cgcagatggg gggccgtgtg ccaatcgcgc caggcgcggc cgatgtcgcc
     6661 gggcatcagc tcggccagcg acagcttcgg gggcagctcg ccgatctcga acgggcgcgg
     6721 caggcgcgcc ttgccggccg cgtccttcat ctcccgcgcc agcgcgcgca aggttttctc
     6781 gtgctcgtcg ggcgagtcgg cgcgcaccgg agcaacgacc cgacactcga tggggccggt
     6841 atcgacatcg tagtagacgc cctgttcggg aaccccgccc aggacgttct ggtcgtgggg
     6901 catgctgtcg atgatttcgc ggaacgcccg ccggtcgggg aaaccgagcg ccagacgcgg
     6961 gccgatgctg ccgaggatcg agtcgaaaac caccgccgac cgggcctccc ccgccaggat
     7021 caggtgaatg ccgttgcggg cgccttcgtt gatcagcgcg cccaattcat ccctgcggtc
     7081 ggcctcgtct ttccacttga tgagattgtc caccaccagc acgatggccg gccggcccag
     7141 gtcaatgccc tgccggcgag cctgcgccca ggtcaaccca tgggcggcca gcaactcgcg
     7201 gcgctgttcc agctcggtga agagccagcg gaacaggcgg gtaattcgcc tcgtctcggt
     7261 gacgcgcacg atggcgccca gatggggcaa atccttgaag acgcccatgg tgttgttgcc
     7321 gaagtcgagc gcgtagaaga ccagctcggc cggcgaatga tccatcgcca gactggtgac
     7381 aagggtgcgc agcggcagct gggtgtgttc ggccatgtcg caacagatga acagatggcc
     7441 gaagcgctcc agatcgggcc gcaaccgggg caggccgccc agtcggcggg ccaattggcc
     7501 cgtcgggtcg tccagcatgc ccatgaccgg ctgcagccag gcatcggagc gcgcccagcc
     7561 gcggccgtcc cagccgcccg ggccgcgaat gtcgtcgagt gcgaccaggc cgccggtatc
     7621 ttccggcaac aggtcgggca ccgggacgat gccggcttgc gccatctcgt gccggatatg
     7681 atcgatgagc gccgtgagtt gggtccgggc cggcctcggt ttttcctgct tccgatgcag
     7741 cacgcgctcc ccgttgagag ccacctggat gacctccagc tcccgctcct tgtcgatcag
     7801 ggccgggtcc gtctcttcat atggaatccc gccatacggc ccctggaaaa ggtcgaagac
     7861 ctcgttttcg cccacttgaa gatagccgcg gccgcgctgc tcaatatagg ccgcgtcggg
     7921 ccgcttcagc actgcctggc tgtcctcggt cttggtgaac ttcatcgaca ggcgaaagcg
     7981 cgagttggcc tgaatctggt cggtgacgat acccgacggc tgctgcgtag acaggatgag
     8041 atgcatgccc aggctgcggc cgatgcggga caggctgacc aaccccgcca gaaagtcggg
     8101 ctgctgcgag gccagctcgg cgaattcgtc gatgatgatg atcagccgcg gcaccgggcg
     8161 cagccggtcg ggggcggcgc gctgcttccg gtgatattgg gagatcttgc ccacgcccac
     8221 gtcggcaaac aggcgctggc ggcgcttgct ctcggcctgc agcgccttca gcgcccgcgt
     8281 ggcatgccag ctctgctgct cctccgattc gccctcgatg ctgctcagga tgctgatgca
     8341 gtggggcaga tacttcaggt cctgcagaaa atcgctgccc ttgaagtcga cgagcatgaa
     8401 agccagctcg tgggggtgat agtgggcggc cagcgtggcg acgaggcatt ccaggaagcg
     8461 cgtcttgccc gagccggacg tgccggcggc cagcccgtgg gggccgtggc cgtcgtcgtg
     8521 gatgtcgaac atcacccgcc gcccgccggc gccgatgccg atggggattt gcagcgattg
     8581 ggtggagtcg ttgcgccgcc attggtcgat cacccgccac tcctccactc gattaaggcc
     8641 caggagatca aacaggctga ccaggttggg gatggccgcg gccgacgatt cgaaccgcag
     8701 cggggccagc agccgcgcga acttgtcggc cgcccccacg ccgacttcgt cggccgcata
     8761 agggatgggc gcggcctggc cgtggcggtc gcgcagcacg ggcgcggcgc ccaccgtgac
     8821 caccgcgccg cattcgcgcg gcaattcagc ctcgcgcgtg gccagaaaga cggcatggaa
     8881 gcccaacgcc gggccgcgct cggtcagcag gcggcgcgtc tcggggttga gcgccgtcgt
     8941 ggcggccacg aagacgacgt agtagaccgg ccagccgggc gccccggtcg cattttttct
     9001 ggcctgcagc tggttgtcac gctggcgcag gatgccggcc agatcgtcga gcatgacctg
     9061 ggcagcagcg tggtcgtcgg ccagatagcg cagctgccga tccggccgcc agacatgggg
     9121 gagccagcgc atccagtccc attgcccggc ttccccgctg gggtagacgg ccgccaggcg
     9181 cagtatctcc ggcgaatgat gggcggccag ctgcatggcg atggcgcgcg tcgcattggc
     9241 cgcgtcgctt cgttcgccgg ccacgccgca caccccggcc tcctgcaaat ccagcaacac
     9301 cggcacgtcg gtgacggtct cgaaccgttt ggccagctgt tcggcctgcg tttcgaacgg
     9361 gtcacgaacc agagggttca tctgctgcgg gggttcgatc ttcacctgga aaggcaccgc
     9421 acccaggccc aggcgaaccc gcagaaagtc gcggtcgttg gcgatgcgcg cccacagccg
     9481 ggtgggatcg cgtgattcgg ccagctgcag gcactcgtcg atcccgggat cgtttttcat
     9541 caggcaggcc tgcgtccggg cgcgcagggc gacggcctcc ccctctcgct ccttcaaaat
     9601 cctttcaaac cgctccttgc gcaaggccac gctctgtctg aattcactct tgttgcgcca
     9661 atagccggtg aacgtgacga tggcgccgcc gagggccatg agcacgccga cgagcacaaa
     9721 aggccccgaa ttcaacaggg cgacggccaa tatcgcgccg gtgatcatca cgcccagcgg
     9781 aatgagcacg ctttcgaggc gaagcttggg ctcgccgggg tgggggccgg ggctggggat
     9841 tttcagcgtt gcttccggcg gcacgcagaa gtcgcgcggg ccctggctaa atttgggtgg
     9901 gtgggtcatc gactccgtca caggggcctc ctgggagaat taggcgggct tccagccgtc
     9961 gtccgcttcg tccggcgtcg ggttgttcgg ccgcggcgca gccgtcaacg ggatccactc
    10021 gacttcgcca tcgtccatca tcggtttatc cggtcgcggc agcggcgcgg gtcgcggcgc
    10081 gggcggcgcc ggtatcatcc gcgggattgg ttggctctct tcataaatcg gaacccagcc
    10141 gaaaatgagt tcatgccagc tgagattgtc ggcatcgttc tgcgagccga tctccaattg
    10201 ggcgccgtcc cacagcccgg cctgggccaa cgacatatcg agcggcaaca gccggccggg
    10261 gccaggccca cggcagcgca aggtcagatt ggccagcacg gccgcctgcg ggttgatatt
    10321 gcgcgtgatc agctcggcca gcttgcgtga gggcacctcg gcgggcactt cgagatcgta
    10381 ctcgaccgcc tggcccttca gcttcacgga aacgatgacg ctttgcatgg cttacctcat
    10441 agatatattg gaagcggcct ggacatcgac ccccggcggc gtgaccgtcg gcgcgatcgt
    10501 cggaacggat gccggcgtgg ccggcggcgc gactggcggt atggccgtcg gaacagatgt
    10561 cggagcgact ggcggtatgg ccgtcggaac ggatgtcggt gcgatcggcg atatggccgt
    10621 cggaacgagt gtcgacgcgg ccgttggcgt gggcgtcggc gctgcttcgt tcaggcccaa
    10681 caatccgctc agtatgggta ccaacctgcc ctgaatggtg ggcctgagaa ccagccacaa
    10741 gatgccgcac agaaccaata gccccaaccc gccggcgaca atgggccaac ggggagcgcg
    10801 ccggacgacg gccgccgccc gcgcgggctg ctcaatcgcc gggacaggcg ctgcgggggg
    10861 cggcaccggt tgtggctcgg gtcgtggcaa cggggccatc ggccgccgtg tcgatggcgc
    10921 ttgttgcgcc ggcgcggcgg ggcgcggttg ggtggccggc tgtgccgaat gtgtggtgag
    10981 ttccagatag accagatcgt cgctgtcggg caggctgttt gaatggtcga tggccctctg
    11041 caaccctaca tcggtcagcc taaggttcgc ccacttgtcc agcaccttga ggccgtcgga
    11101 ataggctacc aggcgcgccg gctctccctg ggcatccgtc agcgggccgt ggtagaggtt
    11161 gaccgcgccg ccgaccggcc cgcgccggct cgaccagcgc tgatcggtct catgaacgcc
    11221 gcccagatcg accgggcgat cgccgcgccg gagataaacg cgcatgtcgc ccatccaggc
    11281 cagcagcacg cggccgttgg ggcgggccgg gccgggcgca tcgacgcggc cgcaaacgaa
    11341 catggcctcg ctgccgcggg cctgcttttc cagcaacacc tcgcgcagca gcggcgggcc
    11401 gtcggggatg gggtattggt ccacgcgctc ggccgccgtc gcggtcagcg ccgacagaaa
    11461 cgccggcagg gaatcgacca gtgcggccgg gtggtcggca accggggggc cggtctccag
    11521 ccaatcgacc agcgcgtcgc ccagcagccg cgcggccaga tcgccataga aagactggcc
    11581 gacgccgtcg cagacggcaa agacaaagtg gtccgccccg tcgcggaagg cgagataatc
    11641 ctgaccgggt tcatcgtcgc ggcgactgtc ggccgcgcgg gcataggcat agcgatagcc
    11701 gaggaaggca ttctccccgg cttgcggcgc gctttcgcgc ttttgatcga gcctgaagga
    11761 tgattcctgg ctcaacgttc gctccttatg cccgccggcg gctagcgggt cgtcgtcgcc
    11821 atggcgaagg ccattcgcac cagatcaggg ttcgtgcccg gcagcaacat cagggcgctg
    11881 ggatggatgt gatagcccat ctcgatcatc atctcgcggt aggattcggg cagcgccgag
    11941 gacatggccc gcagcttgcg tccggcgtcg gtctccagcc gggtgccggg caggatgccc
    12001 ggccattggg tgacgtcgac gatcggggtt ggcagcaggg tgtcggacat gaagatgttc
    12061 tccaccagca ccgggccgtc gggcacgctc atctgcatga tgcgccgggc gatgggttcg
    12121 ggatcgtcgc cggtgtgttc gccgtccgtc agatggcaga cgatgggcgc ggggcagttg
    12181 ttgtatttgt ggatctcgcg cttcagcagc tcttcgacct tggcaaaggc cagggcggtg
    12241 ttggtggtgg ccctcggctt cattttgggg acgccgatgt tggccacctg gtcgatcgat
    12301 ttgatgccgt cgaacgggat aaagagctgg tcgttatagg cgtagatggc gatgtggtag
    12361 cggggctgga tattgctgcc gcgggtcgac aggtagatca tgcgccggaa gatgccttgc
    12421 agggcgtcga tgaccacgtc aatgcgcgtt ttatcgccca gcggctgtcc catcgacgcg
    12481 ctgacgtcaa tgagaaatac ggccagcgcc ggcgtccggc tggtcgcctt cagttcgtag
    12541 ctcacaccaa acctcctgtt tccgtcttgt ctctgcccgt cttttggcat cgatcagata
    12601 ccacaccatt gcacaatatt gtgcaatagc tattgcgcga tggccattgc gccgcgatca
    12661 tcgcctggtc ttattcatct gtctgacgga ctcatcccga tggaggagcg attttgctgc
    12721 ggctgattgc tgtggttccc gcgccgatat ggaacaattg ctgcgggcag ggcacattgc
    12781 caaactgaac ctcactgctc atgcccaagg gcgagcagat tagcgctaga ttagcacagg
    12841 cggggatttt gtcaacaata atggaaatgt caccaggtga tgcggtgaga aacatcgact
    12901 ccgaatgcag ccggacaatg cggtagttat ggcagacctg cgcgccttcg cttttgtcct
    12961 ggcgcgcaac ttgctgacag gtttgttgtc gctgatggtt atttcgttac aatatcaact
    13021 tgagcagggg ctgctcatcg ttctcaggag gattttgcca tgagtgtttg ccctaactgt
    13081 caagaaacaa attccgacaa ctcacgtttt tgccagttct gtggaatgcc tcttcccaaa
    13141 aagcaggtgg aagctctatc cacaactata ccattaactc aatccacatc cattccccat
    13201 cagaaagcgt cgacccagca actcgatcac aacatcggcc aactgggcga cggtggcacg
    13261 gccgggggca gcttatgggg gccattcgcc ggtttcggca cccgcggccg acattcgtca
    13321 tggctgctgg ataatttggg tgagaaagcc ggcgcactcc atgaagccgt aacccagcgc
    13381 tttcagcaaa ggcaaatccc gaacaccagc atgaaatggt acgcccttgt tgcccaaggt
    13441 gtgctggtcg agcgtcggcc cttttacttt gttcgccggg gtatcaccac agtcgcttta
    13501 tatatcgctc agttcgggaa cgacctctat atctcacaag tcacctacgc caaggggcca
    13561 atcagtaatg tacggattgg gatattggca ctgatggccc tattccagct gtttttcatg
    13621 ttccaattca ctaacacagt ggttggccaa atggaaagct atgtcagctc cttcaaccct
    13681 tttggaggtg ggagcagcgg aagcacatca ttgctggcta ttatgttatg ttgtgttggg
    13741 ccgctggggc ttctcaatac gctggcctgg ttgttcatcc tggcgttcag cggcttcaag
    13801 tatctgactg aaaaggactt accggcgatt ttacgtcgcc cgcccaatga attccagatt
    13861 gacgacacca ttgccctgga aaaagccgtc gaggagaccg tgcgtcaatc gctcgacgcg
    13921 atcggcatcg atgcttcatt gatgatccct gtccaggatc ccgatgccgg gcgcacaatc
    13981 aggctgatct aggccatggt caacttaatc gggccgggcc attcacgaat tactcagagc
    14041 gcaggctcac aattagcaac agctattttc ggctatcgga tccctcatct gccggatttt
    14101 ttggccgatc acaccttgct ggctgaggaa tcgcaagaac aattgcgaga ggtgttggag
    14161 cggttggttc ggtttgttgc cggcctgcgc aagtggcgta acagcgcttt ttcccttcgt
    14221 ttcatttcag atccatcaaa ggggcgaatc gacgttgccg tgctcggccg catctccggc
    14281 cacacgaaca ctatccgtga catgacggca caggtaatta ttgatctcgg gatcaacctc
    14341 gcgctctacg gcatcaacgt cgagttgctg gacgtggaaa acacgggatc gagcggactc
    14401 aattactatc tgtatccatt tagtggaacc aataccattg tcgaagttcg gcaacaaggg
    14461 gttgtggttc ccatgatgac tgtccagaaa gaagcctatg ttgtctatcc attttggggg
    14521 ccgtcaacac ccttcctgga gccgtttgaa gcgctgatgc gggtagcagc cccagtggcc
    14581 ataagcattc atctggaacc aaccgagttg acggcggccg agtcggaggc gctcttcacg
    14641 gccgcattcc tggctgatac cgtcgccgac atggatcgga gaacattttc cgacgccagc
    14701 gcgcgacgct tgcgggaccc ggccgcagaa ctggtgggac gaatctatac cgcctatcga
    14761 aaaacacttg atgaaccgtt tctgctcctt tcccaggttt ccggtcccca tccgcaaacg
    14821 acctggacaa tcgcgcgggc cctggcgacg agcatcagca ccggaaatga acgacacgct
    14881 gccgaagcgg ccgaacgggg actgccgagc caggccgcca tcgttgcgcc gggcaatgat
    14941 gccgaatcgg ccgccgcccg gcgcaccttc acctcgcttt atctctcacc ttggggagtc
    15001 accattgcct caccaggaaa agaacgtttc ccctatctgg tcggcgccga gggagcagcc
    15061 gccgcctttc gcttccccgt gagcgtccgc ggcggagtac ccggcatcat ggttcgccaa
    15121 cgcccaccgg attttgaaat cggcccacgc cgccaggaaa tagcagcgga cgagatccac
    15181 ttgggaaatt ttcacagcgg gggggctgta acggtcaaac gtaattcact tctgcgtcat
    15241 ggcttgatca ccggattcac cggctcaggc aaaaccaata caatgctcta tttgctcgac
    15301 caactatgga agcaacatcg ggtgcctttc ctggtcattg aatcagcaaa aaaggaatat
    15361 cgaggcctgc agaatgtgcc cgggttcgaa aaacttctgg tgttcacctt gggtgatgag
    15421 acgaccaccc cattccgcct caacccgttc gagttgctcg acggcgtgcg tgtggaggcc
    15481 cacctgggac gattacagac ttgcttcgat ggcgcattac cccaattcgg aattttgccg
    15541 tccatcgttg ccgagagtct cgagttaata tatcgagaaa aaggttggcg tttgacggac
    15601 cgcggcggcg aatcgaaccg cccgtttccc acaatgcgcg acatgtttcg cgtcgccgtg
    15661 cgcgtgtccg agtcgcgcgg ctatgccggt gagacatatc aaaatatccg cgcggccgtc
    15721 ggtggtcgta tcggcgggtt gctgcgtggc agcaaggggc gcatgttcgg tgcacagcgc
    15781 tcctacccgc tggatgtcct catgagccaa ccttgtgttc tggagctaaa cgatctcaac
    15841 gctgacgata aatcaatgac cagcatgttc ctgctcatgt tattgcgtga attccgcgaa
    15901 caacatcccg gccacacgct gcaacacgtc accgttgttg aagaagccca caatgtgatg
    15961 gggaatgtcc agtcgaccgg cccatcggaa gtagctgccg acaccaaggg ccaggctgtt
    16021 caatcatttg ccgacatgtt gaccgagatc cgcgcgctcg gtgaaggggt tctgatcgcc
    16081 gatcaaagcc cctccaagct aatgcccgac gcgatgcgca acaccaatct tcaaatcgct
    16141 catcagttgc gcgatgccaa agatcgtgag gcaatcggcc gggccatgat tatggacaag
    16201 gcccagcagg attacgtggg taaattgagc atcggcgaag cggctgtgtt tctgaccggt
    16261 atggaaaagg caaccttcat gcaggtgccg ccctacaagg atgacgccgg tctccatcaa
    16321 ctacctgacg atgccgccgt gcgtgcccac atgcagtcgt tcatcgccaa aaacctgacc
    16381 gctcacctcc cgtttgatgg ttgccgcttt tgcggcagcc catgtgaatt cgtagaacta
    16441 atcgaaccac agacgagaga gcctgagctc catgaacagt ttatcggagc gttgggcaaa
    16501 tttgaggagg ccgaatcgga taacgaattg gcggccagtt ggcggggggt ggcgcgcgtt
    16561 tgttacgatg ccgccatacg aggcgcaagg ccacaccccg atgccgccta ttgctatctg
    16621 gcacacgaga tcgattttcc tttcacggaa cacatgcgtc gtgaatttat tcgcgcctat
    16681 gagtccgtga cgcgtttggg ctaagccggg atatccggca aagcaggagg gtatccatga
    16741 caaatgacac aagagacata gccgatatgc cggtggccca gccggagcaa tcaggcaagg
    16801 ccgatgcgga catggccagg atcgaagcgg ccatgaaagg tatgcgtgaa cagcaagata
    16861 gagagttaaa ggaagatgtt gccgccttcg ataacaggcc gaagcccgct tattatcagg
    16921 atagcgcgcc accggccgcg gacgttcggg aaggcgccca ttgggaagac cgaggcatta
    16981 tcgatgtccc cgtggctgac ctgcctcagc cggagggcgt ggatgggccg caggacttca
    17041 atcaccatat caaatatgaa gatgccctct cggcgacaag aaccgtgcag gagatgaggc
    17101 cacaaattga cgctggatat acgagcgatg actttgccga cctggacaaa gcgaaaggat
    17161 tgaactatga gcacggccaa cagcgagtgt acgaccttta ttatggaagc gaccccatcg
    17221 cggtcgacaa ggacggtgat aaatacgata ttgtgagcgg tcgccatcgc atcttcgccg
    17281 cgaaggaaat cggcctggat ttcattcccg cgcgcgtcaa ggaaaaggtc cccggatagc
    17341 taaagacaag agccggggaa attgatatgt caccacccaa aaacgaaagc accgatattc
    17401 tcgtggattt gaaacatgaa ggcacaaaat cggtcgatgc cggattgagt gagaaaggta
    17461 tacgtactat gcagggctgc ggtacagaga gagttagcgc cggccgtaag ggatccgact
    17521 tcgaacaatg ggcaaagacc aatgtcttcc atggcgaagg ccaccgcctg gttgcgctgc
    17581 ctgaggacaa cgaacatctc gataaatatg gcgatggcct ggggcttaca aaaggtcgac
    17641 gaagtgactc ctatattatg gagcggggcg agatatggga actcaagtca ggttacgaga
    17701 agggcggcat cgatcagcaa cagctactcg aatattccat tatggaggac gcaggtcacg
    17761 tgaatatccg caccgcagaa ggtcagctga aggttatgcc agtcaaatct atcaactatc
    17821 tgtttgaaac aaaagatggc gcagcggcta atatgaatag cctgcgcggc caagcaacag
    17881 cctggtataa aaacaatgaa ggtcaagtgc atttatatac cgaagacgcc tgagagcaac
    17941 gtcaagggca aaagcctaat cgcttattgt cagttatgaa gcgatgttca ggaggaacac
    18001 tatgagtgaa acagagcggt ataccccgcc ggaaatgcgc caggaggcaa gcccggagaa
    18061 aacgagcagc cttcgcccgg agacgatcag caagattcag ggggattcac cgaatcatgc
    18121 accaatcacg cccgagcgac aggaacttca caagggcagg gagagtctgc cggagaaaga
    18181 accgggatcg ccattcaaac cttccggcga cccctccgcc tttaagagcg acagttcaat
    18241 gacagacacc caagccccag tttcagatga cccatccgca tcgaccaatg atgacgccgg
    18301 gacggatgat caggcttctg atgtacaata agatcgttag gtaattacag gaggacacaa
    18361 tgccggcaga aatgatatta aaagagacga ccgacgcggc caggaaaagc gtaaaggaag
    18421 gcgtgaacat gattaagaag gcctggtcca tcatggatgc cgcctctgag aagaaaacgc
    18481 cgcaagaggt tttgtcaccc aaagctatcg gcaagattca gggacagtaa caggcaacac
    18541 accggcgtcg cgccttgccc gattgaagac cgctctccgc gagcctcaca ggtgtcttcc
    18601 gcgcggatac tatggcgcat ccggtagcag acgcatggaa ggcaaatgac gacacgctcg
    18661 ccccgaagcc ggttcggggc ttgtttgcat gataaccatc aggaggagtt catgaatagt
    18721 tcaagcagat tagttcgaac catatgcttt gtcttcgggt tgacgctcgt ggtagggagc
    18781 atcggctgca tcagaggggg cgatcaggtc gccatcgaag tcgccgtcgc ccagacaatc
    18841 gccgcccagg cggggtaacc ggccaatacg gccgcgccaa gccggaccgc cgttccgacc
    18901 aaaacagtgg cgccgactga taccgtggcg cccaccgaca cacctgcgcc gaccgagacg
    18961 cccgcaccga ccgagacgcc accccccacc gtgaccccca cgccgaccga gacgcccgca
    19021 cccaccgaca cgccgacgcc gaccgtgacc cccacgccgc tgccgttgcc cgactttgcc
    19081 gataatttcg acgcgggctt gcggccggaa tggaagctgg agactgacga cgcctgggcc
    19141 agtgtgaatg gacggctgac gagtttacgt catggatacc caatggtcgt tggtgatgag
    19201 agttggacgg attacacctt atctcttgat gtgatccaat tgatggaccg ttgttccttg
    19261 tcaatcataa ctgataataa tgatgatggt cgagtggaca aggaactcaa aatggagatg
    19321 aaccccacaa ataatattta ttggcttatc cccgggacca cggatgttat gcccggaacc
    19381 tatatcagtg atatagggag tccgtttaat ctgaagcttg aagtcaagag cgatggtgcg
    19441 gttacgacct tgatcaatga tgaagtcgta acgaatatcg tcatggaggg gtatacccaa
    19501 gggcggatat cgcttgattg tcaccgtcct ggtcgtgatg gtagccgttg gtttaggcca
    19561 ggctcttctt tcgataattt acaaattagt ccgctgaccg ttccttgaac caaccgcgat
    19621 gatctgaatt gagatcatac gccagacgat ttgggttagc tctgtcctgc atccccgcgc
    19681 aagcgggcaa tggccgcggc acgcgtgctg acgcccagcc gcgcataaga ctgggacagc
    19741 atattgcgca ccgttgaatg ggcaatatcc aatcgcagtg ccgcctcata agttgtcatg
    19801 tccggacagt cggccagcag tgacaataaa tcgaactggc gaggggtcag gccggcacgg
    19861 ccgctttccc tctctgccca gggatcgcca agcgatgggc tgacgtaatg gtcacccaag
    19921 gcgagctgtc gcaccacctg cgccaaattc gaccaagcag catgatcact cttcaacaga
    19981 agcccgacgg ctccttcgcg gcgagcacga cgggcatcac tggattcgcc ccgagccgcc
    20041 accagcgcga tcttcagtcc aggataagca cgccgcaagt caggcaggca ctggagaatc
    20101 gggtaaggag tggcatcatc aggtgcggtc ggcgttgcct ggtcggccag caacaaatcg
    20161 gcgccgcccc ggaataacag ttgcggcaat gccgcgccgc agcgcgccac gccggaaatg
    20221 caaatgtcat gctcgcctgc gaggcgataa tgaatgccgt cgatctcgat ctgccgattg
    20281 cttagtattg caattgcaat gggcaagcca ccaatcgcca tgatcacccc cctttgggat
    20341 cacaggacat cttgagacgg cattgccggg atttggcacc aaatttttag gagcaatgcc
    20401 gagagctttc gtcggaaaac gaacaatgtc ccaaacaccg aaacacaatc ggaaattata
    20461 aatgaattat gagaaaaatg caataggcat gtgtaccata ccacgaatat gattctcacg
    20521 tacaatgcga caagttccgg tcgaaattag ctaaagttgc gtaataaaag caaaattcgg
    20581 ctgatttctg ccacttcttg gggtgggcgt atgacacctt tttcgatgcc tcacttagcc
    20641 tgaatgacgg tatctgctct ctccctgggt aatctgcgtc aaagttcaac aagtcggtag
    20701 ccaggccggt acggctgccg ggcgcggggt tgcgttgctc atgatttcgc gcggttggca
    20761 cccgacagaa ataccggcgg gagacgaaag atggatggaa ccgctggagc gattgaacag
    20821 gacatcatca tgaccctcgc cgaggtagcc gagtatctga agatgagctc ctcaaccgtc
    20881 tatcgactgg cacgacaagg ggtggtgccg ggccgaaaaa ttggcggtac gtggcgattc
    20941 tctcgtcgtg tcatcgatgc atggatcaag gagcaagaag cccaccctgc tgctgagcgt
    21001 ttgatatcac tcacccctaa caatgaggca gatttgaatt taacgatccc atgatcttct
    21061 gaggtgacca gaacccggta tgaaccaaaa cgcccaacgt aatcgattga tttgctcaat
    21121 tcccatcttt gtattgttag ccggcttatt gttgccgatg gtacgcgtcc aggcagatga
    21181 tctgccggcc caaaccagtt ccgcagcttg ccacaatctc gacctggtgg tgctagtcga
    21241 ccaatcggcc tccatgcgta ataacgacgt caaccgccag cgaatcgaag tgccgaaact
    21301 gatcgttgag cagctcggca accacgccat cttgctgtgc cctgaccaga acatcgtcca
    21361 tcgcattgcc atttttggct ttggtgatgc ttctcgctac cttggccccg agaacaatta
    21421 catcaacgat acatcagaat acctctcgcc gcagtcgatc ccgctctcct atgaatacga
    21481 cgactgggtc aggtcgcgcg atgccctaaa acagcaactc aacgccttcc gcaacgacaa
    21541 tctcagctat accgatcata aatcggcgtt gttgcgcgcc cgcgatgtgt tgcagatgtg
    21601 gaaacagcag cccgtcgccg gggccgaaga tcgccgacag gcaattctgc tcatcaccga
    21661 cggagaaccc tgcaccggct caggggggtg ctccacaaac ccgggccaat acaaattcga
    21721 tcgcgtcacc tatatgaacg atctgatcca ggtcgcacag ccgtccagcg ttgattttcc
    21781 atatgttgat ggcgacccaa ataacaccat cttcatttct atggttgctc tgtctgacag
    21841 cagccagtcc tacaactacc gtactcagcc gacatttcgc aacggctggc agactatcat
    21901 cggtcagcgt gggcagatct atgacactca ggagagtaac cttgacctgg caacgggcat
    21961 gttcgagatc ttgcgcccgc tcatcggttc caacctgcag gaatgggatt gtcgccgacc
    22021 gatcaacgtc gtcccctatc ttgacagttc gctggtgatc aatatcaacc gccagcccac
    22081 ggactccaca gtcgatccgg cttcgattgc ggtctttctc gatgtgcaaa ccggcggcca
    22141 aacgatcacc ctggaaggcg gtgagcaggt ctgggccgac ggcacgcgcc agccactgcc
    22201 tccgacaatc aagtacaccg ccgacggagg agacgacgga acagtaataa acgaaaacta
    22261 cgcctttacc aatccatggc ctggtgtcta caacgtgcgc acccagggcg gcgatgtttg
    22321 ccatgacatc cgggtcaact acggcaaagg ctcggtcacc gccaacatcc ttcaccccgg
    22381 ggccgacaca gtgctcgtgc aggcgccgca agaaccgtat tacgatgaac tggcgcccaa
    22441 ttatctgagc ctggctgtgc ttgatgaagg tgcgccacta caggaaattg atggatttcc
    22501 actgcatatt cacgccgaga tcgccagccc acaagacaac caggacctgg gcgaactgac
    22561 cctgagcaac aagggcattt atgatgcatc cgaccctatc ttgttgcgcg acgccagcga
    22621 ctatacatgg acagctatcg ggtgcgtaga aagccccacc gaactggtct tgctgagcgc
    22681 tcagggatat gacggccaaa tagtttcttg ttcgtgggcc ggagctgatg acaacctcat
    22741 tcaggtgttc agcgcatcgg gcaatttttc cgtattcccc gtatcatttt tcacttggcg
    22801 cattaccaac ccattggatg gggccatgtc ggctatgaat agcgttcagg gaagccaaca
    22861 ggtacccgcc gccatccctg ttgtcgtcga gctgattgga gaagacggca acttgctccc
    22921 ggcaagctct gttttcaata agcctgatga agcattgcgc gccgaacttt acaaggtggg
    22981 caacgataca ccggtagaga gtattacgct tagcccaacc gacgctgccg cggccgagtt
    23041 ttccgggcaa tttgctaaca gtgcccaggc aggagcaggc gattatcgaa tcgtcgtccg
    23101 gccaaattgg gagccaacaa gtattgaccg caccaaattt gccgtacaga ataatttcat
    23161 cgccgcagag gcggcccact cacagtttga ggtctatcct ctcgatgtac gcatcacccc
    23221 gccgagcgat gtcgcgctcc atagcaccac catcccccaa tgcttcctga agcccggcga
    23281 atgctttggt aatgccgtcc agccgttcga cttcaatgtt gaactggtta acatgaaaac
    23341 cggcctggtt gttcccctgg ccgacgcact ggttgatgtc aacgccgggc ataccctgaa
    23401 agtggctgcg ccgagcgggg ccgaggaaat tatcaagctg aagccgctga acgcggccaa
    23461 tctgcagatg ttgcaagcca ttggcgccgg aacagacctg gacgaggccg gcgagtatac
    23521 catagaggta tcgttggccg gaataccgtt gcgcgacggc ttcagttggg tcgattctcg
    23581 acgcacagct accttttccc gcgaagacac actaatgacc aatccgctcc tggctaaagg
    23641 tgcggttatc ctggccgggc tggttcttct ggctttgctt atctggttga tctatatcca
    23701 caccggcggt cccagtgggc ctctactcta cgactccgtc aacagtctgg accagacgga
    23761 aatagttggc gaatggaaac tctcgtcccg gaagcgcacc aacgtgctaa agagcaagac
    23821 cttgaaggaa agaggggtga aattaatcaa ggttaccaag gggcaggcct taatgaaaga
    23881 aggggcgaag gctattcaaa ttgaagccga agatctacaa ggactgccgc tctacagtgg
    23941 cccgatggaa gatggcgaca tccagtcgtt caatgacggt cggctgcatt atggtggccc
    24001 cataatcaat tagtttccat ttgatatatc tgcatgaagc agaaacatgt cgaatcaaac
    24061 gatagtagtc gttataagtc agttacagag gagataaata tcatgacgag accagcatta
    24121 attgtgggct tgggcggaac ggggcaatgg gtgctgacct ggctcaagcg tgatcttatg
    24181 ttgtcgaacg gcggcacagt gccggataac atccggctcc tgtcgatcga taccgcgact
    24241 acgctggagg cgggtcagaa gcaggtcacg gccaacgata aagaagataa tcaacctgcc
    24301 aaggttggcg gtgtgacgct cgatcggact gaatttatct acgtcggcgg cgatgctcgc
    24361 ccgatagctc tggagacacg tgacaaccga catcctcaac tgaaacggtg gttccgcgcc
    24421 aaatactggc tggacaccca gccaccggcc gtcttcgtac tcgacaatgg cgccggtcgc
    24481 atccgccagt tcggacgcct ggccatctac aaggatatca tggggggcga cgacggtcag
    24541 aaattatgga gcgcatttcg cacggctatc ggcaccgtcg gccggatgac caatgccgaa
    24601 aggcgacttg agatcatcgt cgttgggtct ttcgccggcg gcaccggatc gggcatgttc
    24661 ctcgatgtag gtttgatcct gcgcaagctg gccaaggaaa tcagccccca ccacgtgttg
    24721 cgcggcttct ttgccctgcc gggtgtattc acgcctaacc ccagtcgcga tatgaaagcc
    24781 cgcacctttg ctgcctggcg cgagttgaac cgcttcatgg tcatcgactc cgattttccc
    24841 atgtcgtcag tcgactatgt ggagaatgac agcacctatg ggattgatcc tgccgagcgt
    24901 atcttcgacg cgtgttattt ggtggaaggc aaacgcaagg gggccccagt ggccagcgaa
    24961 gcccgtttcg gcgtttttcc gatggtatcc gaggtgatca gcgccatcct agacgaacag
    25021 gccggccaag tctacaacga ctgggtctat accaacctgg cagaggaata cggcaagcac
    25081 cccacaacgc ccatgtacag cgccatcgga gcctatactg ttcaggttcc ggccaacttc
    25141 gtcgaggtga tgtccagtca tatcttcggc caagagatgc tcaagaggtt gctcagcccc
    25201 gaagtggctc ccgacgcaaa caccaagaaa ctggtcgcct ccggcgcctt gcgccatctg
    25261 acattggcgg ccaaagctcg caacctggaa gaccccgggt tcgccggtcg ccagcgcgcg
    25321 gatactttcc tcaccagcga gagcgcctat gaggggcaga tggtcaatcc gacgatcttc
    25381 atggcccggg ccgccacatt gctctcggag gctcgtgaac aaggaaaact gccgcaagtg
    25441 atcgatcgac tggcccgcgc cggcacaatg ggcggcggcg gtgcagaagg ctgggcgcga
    25501 ttttatcctg agttcggcaa tgaccccgaa tttgaatcac tcctccgtga cgtgcgcgat
    25561 catacccatt tcaacatcgt cactgccttt agtcgccgcg aaaaggaaaa ggccgaagac
    25621 ttccggaagc gcatcattca gctcgatccc ctggtccaga ccaagttcgg atcaagcaag
    25681 atcggaggcg accaaatgat cgagtacgat ggatcctttg gggtagtact cgatcgcgtc
    25741 cacgagaccc agatgatcgt cttccgccag gcattgcgct tgcggctact ctcgctcctg
    25801 atggggcgcg acgcgaatcc tcttaaagcc cgtggtggca agctgggcta tacctgggat
    25861 ttcttcgacg gtgttgccgc taatctggat tggttcctca accaggtaat ggatggagtg
    25921 tccaaacggc gcaaagaact ccgcccgtca atcgacgctg agattcgtaa tcgcaaagcc
    25981 gaagattaca tggacaaggt acgggacaaa aggttcctgt tcttgtggga aagcccaagt
    26041 gttaagaagt ctgaaacatt atttctgcgc accgagcagg aaaagatgga catacgccgc
    26101 gaggatatcc tctaccgcta cgtggaccgg acattgcgtg caatgaaagc tgccgcggaa
    26161 cagacgcgcg atgctgtcca ggcctggatc tggcacctct caaccggcga cgatgccagc
    26221 gaactccccg gcatatggga tgagatgctc tccagcgaag ccaatgtgaa agaagctcat
    26281 ggctgggatc aaagtaccga caccgtacag cgaatggtcg ccgaagaagt actgcccgtc
    26341 agcgaagaag acctggctaa tatacttcgt cgatggcaat gggacgccgg ctatgaacac
    26401 gaacatctct ggctgaaggt caatattctg cctgaggctg agggtgaacc gatcgacgtg
    26461 ctgaataatc ctgttcgtga gagcctggcg cgccgcgatg aagtaggcgc tcacaacacg
    26521 agccgcttgc tggggttggc tcgacgcaat tacgccggcc tggtcgggcg cacaacagtt
    26581 gccgagacga ttatcgacga atacgggcct aatggcggag ttcgatttgc caatgacgtc
    26641 gctaccaaga acgccgagcc gttgttcgtt ggctcaacaa ccaacaaacc caaacgccaa
    26701 agtaatctga tacgcgtgaa gtcgcctgcc aatgatcagt tctttaccgg gaaagacagc
    26761 gcccagagcc agcttcgcgt gctggcccag aaggatccgg tgaacgctga tgccaattac
    26821 ctgattcagg tcgtcgggtc ggagaacccc ttcaaattga cgcttgttcg ctcggacgat
    26881 ctctacaatt tcgatgagtt cgcggcctgg gaagattgcc aatcagcgta tgcagcacat
    26941 gtaaacgaca aagagggact gatgcgccct gtaaatctcc acaacttcgc cgcggaggcc
    27001 aacgctgtcg aattcgagac gcgtttggtg aaggacaaga aaaaaagtta ccgtcccctg
    27061 catcctcgcg ttgtcatgtt gctggagaac cctgaaccac tgcgccaatt tctctatctg
    27121 cttatactgg gcaacatcac cgaagcacac gatcgctctg cctatcattg ggaattatcc
    27181 tgggatcggg ccgacggcac ggagacgatc tacctgacca aaccatggaa cgaagatgaa
    27241 gaacgagctc atagcgcaaa gccggacatc ttcaatgcag ctcatggata tgtcatccgc
    27301 caaaaatcgc acgagaaata tagccaggcg gaagttgata ttgaattcgg tcggatttat
    27361 atcgatcgcg ccatggccga tgcgggccgg caggccgtac acgatgtact gaataacgaa
    27421 ctgaatggca aaggcgaaat aggcaagatg gaacgagcgg cacgagaaag ggacaagaac
    27481 accatcattc gtcaagacct ggaggatctg gctatcgtct ttcgaatgat gctgcaagat
    27541 gacctgagtc accaccagcc ggttatagaa gagcgtcgag agaacgcctt tgagcgtgct
    27601 cggcgcctgc gcgaagaaac cgaagcggcg gcagcagccg aggcgaccaa ggctgccaaa
    27661 gctcgatcca agaccaacaa gaccggagga agcaccaaaa cgacctaatc aatcgccaaa
    27721 ggtcttggac cagaaaattc cgaccggaaa ccctccccga acgactgaat gatcgttcgg
    27781 ggagagccgg ccgtatcgcc gacaggccgg cgcagacacg gaaaagcaac ggcctgtaga
    27841 acaggaggca atcaattcag tggcaaggtt ggcaaagata atccttggtg cctggttggc
    27901 cgtcaccctc attctggccg tgccgatcgt catcacgctg atacgcggct ccgtatccac
    27961 cgattcattc agtcgttcgg ccgagccgcg gatcctgttc gggcctggcc caagccatgt
    28021 tcaggtagat ttcacccccg aatctaccgg ctgcccggtc attcccctgt ccattcttct
    28081 cctcatcgat aaatcgggga gcatgaatga cggtaatgcc tttcctctgg cacgtgaagc
    28141 ggcaattgct ttcatcaata atgtcgatct aagccagtcg acggttgcca ttgcctattt
    28201 tgatgacaca gcccatctca tccaggcggc tacacaggac agagatgcct tgattaccgc
    28261 cgttaatcag gtcacattac cttacgatgg gacggacctc acgagagcgt tgcagtttgc
    28321 tgcgagcaac attggttctg accggcccgt tgttgttttg ctaactgacg gcggcgcaaa
    28381 tgatccggcc ggggccatgg ccgcaggaga tgctctcaaa tcggccggcg cccatttggt
    28441 caccgtggct gtcggcccgt tagctgaagt cgactatctg cgcagtcttg cctcttctcc
    28501 ggctgcagca ctcgcatccg acgacccggc agacttgcaa tccatctata ccagcctcgc
    28561 gggacagcta aacaatatcg tcgccaggga tattatgcta accgagccag tgtcggaggg
    28621 cctaacgatt gttccggaat ccgttgcccc attggccacg cagaacggca atgatcttgt
    28681 ctggcaagta ggctttatcc ccgctaccgg tgtgaccttc cgttatgacg tcacaactga
    28741 aggtatcggc tggcctaaaa tcgcatcgga gacagcaacc atgtcgttta tagattgtca
    28801 ggccggattg gcctctcttt cgctcggtgg cgggccacgg atcctgatat tgccggggcc
    28861 ggcattctgg atcccctggc tcctggcgtt attagtgccc gtgttcctgc tgtttcgcgg
    28921 caaaaagcaa gtccccccac ctgtgaccgt cgccgcggcg tcatcggtcc ccatcgcatc
    28981 tcccgatccg acgccggcct ggctgcgccg gcttgattcg accgacaagg tgttaggtat
    29041 ttccgaggtt gaatccgagt ctggaaccct tgtgccgaca atcatcgttg ggcttggacc
    29101 ggtgggtcga attgtgcttt cacaggtagc ccaggcgctc aacggccgct tcgcccgtcg
    29161 cgataaacta ccagtacgat tgctgcaagt ggatgtgcaa cccacgagcg gccccatctc
    29221 cgaacgtcct gactacctgt tgcccgatga gtgggtcatc ttgcgccctg actatcagtt
    29281 gatcagtcgg acgctgcaaa cacaccccga cgaatgggag cacatgaact ggtatgagcc
    29341 gacggccgcc gccgactatg ggcgggctcg tgggcgcatg gctcttttct atgacttgcg
    29401 taatggaact gccaattcca ccctctatcg gggcctcatg aaagccgcag acaacatggt
    29461 caatcctcgc ctgcgtgtta tcggatccac attcgatgac gtcagcagcg gcatgctggt
    29521 cgatgtttcc tggctgatgt ggctggcgac caaacgccat gtcgatgtgg agctatggct
    29581 tagcggccca ttgaaccaaa attggtcgcc acgcctggac aacgcaaatc ataccgtcac
    29641 cgttgatgaa cagcgtgcgc gcacactggc aaccctgcgc gagattgagc gattccaacg
    29701 taacgccatc gttccccttc actatgtccc gcatgatatg gggcaggagg agttttatca
    29761 ggaagtcgat tcggccgtcg tccaaacgct ctttctcttc gaagcgccgc aggacaagaa
    29821 aatcgttgtt gaagaccacc tggcaactct gacagacagt ctggtggctc ttcttaatcc
    29881 ttctgtgcag aaagaggtca ctgaacatct ggtacgcttt caggccgatg ctttcgacct
    29941 ggttaacagg gatggccacg gcctggtgtg cggcataggc acctatgcta tcagcacgcc
    30001 attagcccct ttgcacgaag cattgacctg gagactagtc cacgatctgg tctgcgaagc
    30061 gcgaaccggt ttgctgccgg cacgccaatt gggacttgac ggcaagtacg aaacaaagaa
    30121 acccgatgac atcatcagct tcaccgtccg ccgaacggaa attgaggatt tcattgacaa
    30181 ccggcgcgcg catcttcacc atgatgcctt tcgacaggct gtggccgcac aggtcgccga
    30241 tatgctgaac ggcgaaggcg gtaaaatccc ggcgatagat cggtccggcg gcatccgtcg
    30301 cgcccggaag tgggttgaca tgttgcgaac cgttctcaag caagatggcg aggagatggc
    30361 cgcagcgcca ttggccgatc tcatccggga gcttgatgaa tgggataatt tcctccacaa
    30421 agacctgtcc actttggcca ccgagcggct tgcggccgcg cgcgaacgcc ttggcaaatt
    30481 aacgtcccaa ggcgggcgat cctggtcgat cgacgccgaa ctggagtggc ctgcctataa
    30541 agctcacatt cgctcatggc ttgatcggcc gtccaccgct ctggcgggcg aggctatcgt
    30601 acgcgtctgt cagcggttcg gctggcaggt aacctatgat ccgactgcac ctggatggca
    30661 agtcgaattg tggattcccg atggcgagtt tatatgggcc ggccagccca tcgcatcagg
    30721 tggcctggcg gtgccacagg attcggcaaa ggtcctcgac cacctttatc atcttgcccg
    30781 gccgctggct tacaacactg agtcacacgg aaatgtgtta aaacgagcgg ccgacatgga
    30841 tagtgttacc tggctgaatg ctgccgaacc ccgattgaga gtcaacgacc tgaccatctc
    30901 caaactcctg aagggagcca atagcgttca cccattcctg gccgcgcgag aaaccgacaa
    30961 cacaccaaaa atccgccagg gcttgagcag caccggcgat gcccagcagg tcacagcctg
    31021 cgccattgaa gacgccacaa cggtgaccct gttgcgtgta cgcgatcgtt tgccgatgag
    31081 cgccacttat ctttatggcg aagaccgcga cggcaacagc gcctggaatc aggctatcgt
    31141 cacccccgcc cattacgtat ggcgtggcga gcaggcggcg gccgagcatg aaatcggaga
    31201 acacctcggt tcgcggttcg ttggctatct ggagcgcgat gaaaagcttg tcgatctgta
    31261 tgcccgcgcc gtgctcttcg gcttgttcga ggaaactcgt gacggcgttg caatcggtgg
    31321 cgcgcaggga tgggcctggc cggggaaaac agccggtgat gccttggtca atctcttcaa
    31381 tgttgattct acgcaaaggc caggtgttct gaacgaaaga ggtggagccg gtcgcatgga
    31441 ggcgtttcag gctgtggagg aggctattga gcgatcggta gaacacatcc gatcatctac
    31501 cggcctggcc caatttcgcc gcgaagccga acgagtcttg cagaaacgcc aacgctcacg
    31561 catcgaactg gaatcagtcg aaccctggca gcgtgattat gaagattact ttcgcccgct
    31621 ggctaaggat gcctcttctg aagaccgtga tctccgcttg tatttgttat acatcctcag
    31681 cacgctgcag taaaaggtga tgctatgcca cgattgaata aagaccatgt attgaagagt
    31741 actaatgacc agtaccggat tatcaagtac ctgggcgagg gattgacggc catcgtatat
    31801 gaagccgagc gtctcactgc caatgccaat ggccttaact ttccgatcgg cagtcacgtc
    31861 gctgtgaagg tcctgcaaga ggggctgccg gatgatattc gtcgcaactt ccgcgatgaa
    31921 gccgatgtaa ttgccaaatt aagccatgag gagaccaaag ccgggttgaa gcggtcactc
    31981 atacctaacc tggtcgagca atacctggga tcgggcgcag gaccacagtt cttggctatg
    32041 gaattcgtcc agggcacgcc tcttgatcgc cgtgtgcgag aaggagaccc gctgtcggaa
    32101 cagatgggtc tccaggtagc tgactcggtg ttaacagtac ttgatttgct ccatactcag
    32161 ctgcacaaat cgtatactga tttccagttg cagaatgttt ggcttcttcc agaccagcag
    32221 aatattaaaa ttatggactg gaatcacgtc agcagagagc aaaacccgct cccatatgag
    32281 ctggtggatg cggatctcag acgaatggga gcctacctct atcagattct taccggtaag
    32341 ggtgcaattc aggacggtga aacagctgaa gcattgagca ggcgagcggg taaacattgg
    32401 gataatcttt ccgtcggaac gcgccatctc atccgccgat cactcaaccc caatcccgcc
    32461 aaccggttcc gtagcgcggc cgagttccgc gacagggttc tccaacaaca ccgattatgg
    32521 gaagcgaata ccgatagcgt cgttgatgaa gcgcagactc tcatgagacc tgtcacaaaa
    32581 gccatcgccg acggttctcg gctgtcaacc gatgaactga cctcggctga gacctgggtc
    32641 gacatggcag accgccgtca tcgcggcgat cagcttgttc aatattggaa agagactatc
    32701 agtaaagcaa ctcgggacgt atcggctacg tggggcagtg gaatgcaact atttggcggc
    32761 aagcagtatc gggaagctgc cgccatcctg cgcaacgaag cctcgtcctg gggacgagcc
    32821 gacttgtggc gacaggtcat gacggccgac gcggctgtcg aactgggcga cggtctcttt
    32881 cagtcggtcg ccgctgaact catcaaggtt gtcgacacga tgaatagcgg caattgggag
    32941 gaggctcgcg agctgtttgg cgcgccgccc ctggctgccg cccacggggt ggctataggc
    33001 tccctgcgag gcgagatcga cgcccaaata gcatatcagg cagcgcgccc ggcctttaat
    33061 gaaggtcgct ggaacgcagc tgcccgcgat tttcacatcg tgaatgaggc gctacctaaa
    33121 atcgcctatg ccgaggtatt gcgaacgcaa tacggctggg atggggtaga tgaattggcg
    33181 gccgaagcca aacgtctggc ggaagatgtt gagcgcggca atgcccagat aagagaactg
    33241 actgacgccc tctctcgcga tctggacgac ggcttggcgc aaatccagga tgcccttctc
    33301 aggcgttctg actcacccga gatcatccgc ctttgcgagc gggaagctga acgccgcccg
    33361 gcatcggagg cccagcgctt gctccttacc gcgctgactt acggagctgt ccccgctgcg
    33421 caggaagttg cgttgaagtc ggcttacaaa gaaaacgaag agagaatcaa aaaagagcgt
    33481 cttcaggctg aagccgcgca acgagaggca gccgaagcgc gcacgcgaga agaaataaat
    33541 tcccaaattc aagcaggacg atgggctgaa acggccggct tgcttggggc aatcccgctc
    33601 cggctgtcgg acaacgttat cgatcaggtt cgttcggcct tcgatgcggc cgtcgacgga
    33661 gagaacagcc ggcaggcgcg ggagatcgct gcggttctcg acgtcgttga gccggggaat
    33721 gccgacgtcc gccgcggccg tctcgaggcc cagcaaacgc gacaaaaggc cgccaccgat
    33781 gcctggcaaa cgaacctgcc taaccaggtc cagggtatta tagctgccgg ccaattcgat
    33841 gaagcccggg agcggattca tctcgccctg cccctgctgc cggaaaaatc tccaatcact
    33901 tcgttcttgc gcaaacaaga gcagcaaatc gacgagttca cggcagccca aaccaaactt
    33961 gacgatatcc acgacaaatt cagcaaccgg ggcctcggcc ccgatgatgc ggacaaagcg
    34021 ctcaatctac tggatgggcg catcgctgca ctcggcgcat cgttgacacc cgcagcgact
    34081 caacacctta aagattccgc cgacgcgcta cgatacgacg tccggctgcg tcgcctcagg
    34141 aacgaactcg atgaagccgt taaggctatt agcgcgaaaa gctacgatac cgctgaaagc
    34201 aagcttgccg atgctgaaac tgtactcaat agcgtcccat ccggccaccc cggtttcagt
    34261 gagcagcaag agcgcttgac gaacagcaaa tcacgacttg agagggaacg ctcggccggg
    34321 aagaagtttt gggatcgatt cggcaaatgg atcttcggcg cgctggttgc gctggcgttg
    34381 gtgctggcgg ccggggccgc gtacggattg gtgtcggcga accgggaacg cgacgttcgg
    34441 gccacaactg agtccgcgct ggctacaact gatgcaaact acaactttgt cttcgccgag
    34501 ctggaaaaac tacggcaacc caacataaat gtcacacaga cggttgaagc ctttcagacc
    34561 cgtgcagcaa ccgatgcggc agcattgata gtggctaacg ctgccgcaac ggcgctggtt
    34621 gcgtccaatg aggggctgag cggcacggtg accgccgccc aaaccacccc ggagccgttg
    34681 aacctggtca ccaacctggt cggggccgct ccagtgaccg gtacgatagg cgaagatgtg
    34741 ccggtgtttt acgatcttcc gattagcatg aatgtgaccg ctccacccga ttggtcattc
    34801 caaattgatc ccgagcgtgg tctgcagctt gtcgatcctg gcgagaccgc ccatacccta
    34861 aggttggtcc acacctctct tagtcggggc acaaccatcc agacagctgt cccggcgtcg
    34921 agtgtccagt tgaatccgga cacgccgcat gaggcgacga ttgccctggc tgccctcccg
    34981 gtcgttttgc caaacgactc cggtcactat cgccttggct gggaagccgt ggccgcggat
    35041 gacgcgaaca atcgcagggt cggggcgtcg gtggctgagt tcgagataat ctccccaccg
    35101 acagtgatga ttaaaagcga gagattatat cgcacgcggc cgttatggga ggacagtttt
    35161 atcgtgccgg gaactggcga tctgacagtt gaggtattgg gcaagctgga ccttgcggac
    35221 gagcaaacag gggacggcca aacgccgccc gttccggatt tcctgatgat ccgactgcgg
    35281 ggcagccggg agatcttttg gctgccgtcc tggaacgcag tggaatttga cgatagcgag
    35341 aagtggcaag ctctgctcga tagtctgcct gaagtgtcgg cgccagacgc ttagatcgag
    35401 aaggggcagc agctccacca atgcgcgatc aagaggaata atgacccacg aagagaaaga
    35461 aagcctgatt aacgacctcc gcctgtggat ggaaggtgta gacgacgacg agacttatgg
    35521 ccgaatgggg gccgcgctgc ggcgcattcg cgcctgggat ccctccggca acgatccaat
    35581 accctataaa agtgaaatcg agcaatgggc caatgcccgt ggcggagcga gcgcctggcc
    35641 accctattcg gaaacccgcc cgatgccgga tgacactcaa tcaaaagagg atgagccgct
    35701 atccaccata gcgagcgcgg aatcggctga gtcggcagac ccggatgacc acctgcaaga
    35761 agcctacgac agcgcgatgt tgcgaatgga gggtggggca tattacgagg ctatgcaggg
    35821 tttccaggac atcgccgaaa aggcccgggg ccgcttgctc cgaccagccg aagagtacca
    35881 gtttcgggcc aaggccgagc tggaacatcg gaaagccccc ctgctggata tcgctcgcgc
    35941 tgccgcgccg gaggcggccg aagcagcctg ggaagctgtt cgcggtgttg atcccgacga
    36001 tgttgaggcc gcggccgctc tggagacatc ccaatcccaa gccgtggtcg aacgaaccag
    36061 acggcgtgcc gaagaggttc ttgatgaagc aaattcggca tttaacaggc gaaacctggg
    36121 ggccttgaat ctgctggtgg gcgaagtcga cacgctgcgg cgtgccaata aggccatcgc
    36181 actgcgtcca gtgctggatg atctgctgca ggacgtgata caacgccgcg atcaattacg
    36241 cgcccagttg ggttccccca gtaccctcat tgccacgggc aacaaccgcg aggcgtatgc
    36301 tcgagccagg cagatgttcg tcgaaaaaat cctgacaatt gtggataccg ccggcatcat
    36361 gggcggtgaa ccgggggccg aggtggaaac ggcccgtttt ctggataagg ctcgggaaat
    36421 ctatttggct gggttgaaca aattcgccgg cgaccgcctg acggtggctg aaagcgagca
    36481 aggcgtggat ccatccggcg ccctggagac gctcaacgaa gtcgtttcag cgctgaccga
    36541 tgatatcctc accggtgatg atcgcgtcca tctgcagaac agcctggata ccattgaaaa
    36601 acggatcaag tccgtcgaga tgcgcctgcg cttatatgaa caagcacagg cgctggtggt
    36661 ggccgcgggc gaaccagggt tggccgccga cgacaagttt ctgaaattgc gccaggcccg
    36721 ggaaatctac accgagtacc ctgaaatcga cgacaaattg cgggcggccc tgacggatgt
    36781 ttccgccgcg gcggcggccg atctggaagg cgagatcgcc ctggcgaaag cacaaatcaa
    36841 gcgggatgat ttcaatgaag cgcgccgcat tctgcaagag gcgcgcgtca aggcaaccaa
    36901 taaggtcccg gatcccatcc caggttcatc gcttcatcag cgcctggatg aactgagcaa
    36961 gcttgatgac gagatatcag atgccgaagt agcctatgaa cggctgatgt cgcgacttgc
    37021 cgggatcaat aacctgctca atgacgaaga cgtcagtcgg caaaaggcaa acctaatcga
    37081 agcacgctcg cgtctcgaat cattgtctcc cagcgataca aatcgagagg aagtcgtgac
    37141 cctaaaaggc cgcctgcgac gactccaggg cctcgattca aactggaccg aggggcagcg
    37201 tgcttaccag gccggtcaat ggacaaaggc gattgatgag ttggcgctcg tcgctgccgc
    37261 tgccaatcac acggccagag acagggccga tgttatggcc aaacgagccg aagcagcaca
    37321 gcaggttgag gtggctgttg aggctgagaa ggaaaggcgc ttcggggatg cctacgccgc
    37381 ctataccgat gccctcactc gcttcaagca atacgatcat gacccacaga cccagggcac
    37441 acgccgaaca gccgaggcgg ccatagagcg gctcaaacca tacaccgatg gagatgtaga
    37501 ggtcggtaaa ttgcgccggg atgctgcggc acgattggcg caagctaaag caatcaccga
    37561 ttcgcaacgc ggttcaccct cacttctcca gcccaatcag ggttaccgtg acgcgattgt
    37621 gaagctgggc caagccgcaa aggtggaata caccaatctg gggccacagg tggaagctga
    37681 cttgcaaaaa gtccgcgctc agtgggaaga agccaatagc gagggaatgc tactggccgt
    37741 cggttccgac gacccggagg tgttagaaaa gggtatcaca ctgggtcagg agctggaaaa
    37801 cgaaaagctc ctgaccaagc caaccaatcg cgacttgcta cggcgtctca gactgaaggc
    37861 ccttgacatc aaataccgac gcttgctgag taaccgatcg acatctcccg ctgagattga
    37921 gactaaccgt cgtgaccggc tgaatcacac cccggatgat gccggtgatc ggtcggaaat
    37981 tgaaaagcag ctatcggagg cagtggccaa ccgggtgatc gtcgagatgg gccagaagta
    38041 tgattccggc ggggcagacg cagcgatgaa atacctcagc caagagatga accagcccga
    38101 agtttatggc agtcgcgccc tcttccagga gttcatgcag ttgctctggg aggaagagca
    38161 ctgggatgat gcacgcaccg aagcggatcg attacggttc cggcaattgg cgcggccgac
    38221 cgaactgcgc caattgtggc acgacctgac cgacatcgcc gagagaatgt gcgcaaatga
    38281 caagaaggcg ttcgaggctc agctcgattc cttaaatgaa tggtttctcg ccggcagcga
    38341 tcattcgggc attggtgggg ccggcgggcg caccgcctac gtctggttta ccgatcagaa
    38401 tgagcgacgc gcgtatataa acgacgagat agcgcaactc aagcgcaagc gcgtggataa
    38461 actggtcagg caagccatca ccgaacaagg caccgggaac ttgctgaagg tagcacaata
    38521 ttacggatac gctcgtctct ggaaccctgg agatcctcgg gtcatcaacg ggttacaagg
    38581 ggtgggacca caactgcaaa acagcctgca ggcactcttg acccgtgccg ggaacgtgac
    38641 attgcaggcg aacgcgttcg ccaaaagcct ggaggatgcc gacgagataa ggaaacaact
    38701 ggtcgatatt cgcgaagtcg gcaacgcgtt ggggctgacg accaccacca tgaatcagct
    38761 ttcgatcacg gccgaatctc tcaaggagag gaccgacggt tggcgtcagg tagagatgat
    38821 cttaaagggg atcgatgtag atgcgcgcaa ggcacttcga aatccagccg gtatccagaa
    38881 cagtcagggg ggctgggatc tgacaaacgt gctgactcaa ttttccaatg ccgtaacaga
    38941 ggcaaacaag caagcaactg atcgcactgc gctgaatacg atcatcaaca accatcgtac
    39001 tgacctgaat aatctaaatt ccgacgcaga cgtcctcaac aaagacgtgg tcgcctttct
    39061 ggaagccgtg gatcaagagg acttcgataa ggttatcagt ttgggcaagt ctttggatac
    39121 aacatggagc gctcgtcaga gcagcgggtt cgacggattg gagggactga ttacccgcaa
    39181 aatgttctat ggctcgggcg agttgagaac tctgaaagcg cacatcacca cagcccagaa
    39241 ccaaagccgc gacttggcca gatggcagac ctgggccgac aagaccgttg ccgcgcatga
    39301 tgtgctcgat acggccgtat cgcagcttaa caaaccgatg gatgatttgc gcgatacgta
    39361 ttccctgaag gaactgaagg agatgtgcga ggcggcgctt aatttggtgg aacagttttt
    39421 gaatgtggca ggccaaaagc cggaaagtaa cccgttgagc aagaaagcgc aggaacaacg
    39481 cacacggatc ccggatgact gggaagaaga tctgatagca ggcggccctg aaaaccctcg
    39541 ttctatcatc ctgcagcgaa agggtgagat catggacgat atccagaaat tgagcggaca
    39601 gaatggacca ttgcaacaac taaatggggc catgacgcag ctaacaaacg cccacaagga
    39661 agctcaaggc aaaaacggcg ggcttttcgg acgaaaaccg aaaggggttt cagataactt
    39721 gttcaaaaat gcggctcgtt tccttgaagc gtgccggaag ctggacccgg ccaacccgga
    39781 tgttcgtgat gcccgaaagg gactacacca gttggcaaca ttgtataaca agcaggacct
    39841 gcttaaagag aagtgattat gtcttcacgc ttcgctttgg ttactgatat tcatgccaac
    39901 tggcacgcgc tgagggcgat catcgacgac gtggccgatg tgaatagcag ctgcatagat
    39961 aacccctatc gcttctggtt cttgggcgac attatcggct atggaccgca tccggtcgag
    40021 tgtttaaact ggctccgcta caagagccgg atcggtgacc gttgggtacc cgggaatcat
    40081 gatgcctggc tgatcaaccc cgaaggcgtc aaccctgatg ccgacgaaag cctaaaacgc
    40141 caccgcgacc tgttggccga agacactcag tctgaattgg gccgttggtt tcgggaggag
    40201 ctctatagag cgctaggtac aggatttggg atcgacccaa acaatgaaga accgcgctgg
    40261 cgggtatgcg aaaagagtag cgattggacg ttgttcttcg tccatgcgaa tcttttcgat
    40321 agacgacgga cttatctttg ggcgtgggat ccgggccagt tgagtctgga gttcggtcga
    40381 atgaagaaag aggtcggcga agacgcaaat gcagttctct tctgtgggca cgcacattat
    40441 ccgctcttcg ctcgccaact tcccaatccg gatgagagtg ttgccctgca gtccattcga
    40501 tattttacca gactaaagat tcaacccggg ctgacgatca tcaatcctgg cagtgccggc
    40561 caaccacggg acggcgactc tcgcgccgcc tatgcgctct tcgatgccga ggatatgaca
    40621 attgagtttc gccgcgtggg atacgacgtc gataaaacga tagccgattt ggatgagaaa
    40681 aagtacccaa aatccttagg agagcgcctg gctactggca acggcaagtc cgaattggct
    40741 tcatactttt cagtctatcg gcgaccgcgt tgggatctgg aggcttacga aagctcggcg
    40801 cccggagagc tacccgagga acctgatgag cattggtacg ggagaagagg tgatgcctag
    40861 gaaaaacgat ccattcgctg atttagataa catgtcctgg gatgccgact ttgcatctca
    40921 tgattcgcgc agacagaaga ttttcctgat agtggtcggg ctggtgatta taggcgcgat
    40981 tatgacaggt atttattacc tgctaagtgg gacgctgttc gattcgccgg agcgtgcggc
    41041 cgaacgatgg ctaaacgcgc tggctaaagg cgacaccgac cgtatgctcg aattgacctg
    41101 taatgatcag ctccagaccg catcgattct cagtttgcct gaaatggtcg gcggtgatgc
    41161 cttgcaggaa ttggggttgt cggaatggct gaaaaatatt gatttcgata tgggaaacct
    41221 gaaatatacc ggatctgagg tttcagccgg ggaatcggaa gtgatggtca ggggtatctt
    41281 gcgaatcaga gccatcaatg atttctggct gccgatacca ttgaaccaac gctggcgaat
    41341 gatagaagaa gacaatgctt ggaaatggtg cggctatata ccccaggcat agaaggagac
    41401 atcatgaatc ggatatttgc cttttttctc tttattatcg gcgcggggct ggttgctatc
    41461 ggcgcgtgcg gcaacttgac atacctggtg tccctgaaca cttggggcat catctggcgc
    41521 atcggcctgg gcgtgctgct catcattctg gccatcattc tggcgaatgc ggactcgatt
    41581 accggatcgg atgatccgtt ttaggacgga ggaacccaat gggactggat atttttaccc
    41641 aaatgctgat cgtgtttgct gtgatcgtgt tggagcaggt aatcgtctgg tttgctgaag
    41701 atttctggga gttcaggaaa cagataagcc aacgtgggat aggcaaacga ggtgggtcat
    41761 gagttccgat gagtgggagc tgttgtcgcc acgatatttt agcgacattc ttgttccatt
    41821 atttaatggt gggctaatcg cctatgccgt catttttgtt ctgctctttt tcggcaccat
    41881 catcatgcca ataattgtga agctgattag aaaacaagcg acgcaaagat atgcaaataa
    41941 ctcatcccag gttgacccga accacaaaaa gacgttactg actctcacgg acattcctca
    42001 atatctgccg gacgatcgct ggagctacag gctattctac tacacctcct taagcttggg
    42061 agtattatat ttttccttcc atatcgccat cagtgtatgg cctacgacta tattaaccac
    42121 gggcggaaag ttgcaattgc tcaccgctga cgacattgaa gccatgatcc agtttctcta
    42181 tcgttggata ctcacgggtt acgcgctggt tgtgttctct atttctttgg ggcttgattt
    42241 tgtagcccga tggatggcgg tgtcatggcc aaaggagaag cagcctgata cggggccgca
    42301 ggagccggct gctcacccgc cttcaccccc aacaccagca tcctcgacaa gcttgccgat
    42361 gggtcaaaca tcattgaaaa acgtcatcac aagcataaaa cagtcatctt tcctggttga
    42421 aatcagaatt gagaagtcaa atcacagaaa gaagggtgaa gacaagttaa tgcatgatag
    42481 ctcaagagca gtatgtgttt caaccaaggg tcagattgaa tatgaggacg aatggccaac
    42541 tggagacgag atgcaacgct tacttcgctt tcgatcaaat gacggcgtgt ggtgcgtcgc
    42601 ttatgttgaa tctccactcg tcggaacaag tgttaaatat ttaaaaggag gtcgtggtcg
    42661 ctggcaacta ttgacaaatg aaatgccaat taacaatgag atcatcaagt ttcagtgggg
    42721 gatagatggt gttcgatatt attgcgaatg cacgcgttat gtcaaactat agaatcgctt
    42781 tcgcttgctt ggcttgcata ttctcgcttg ttgcggcgaa tgcatcacat gcccaacagg
    42841 aagcgaatac ctataccata caatttttgc ctatgggtga aaacggagct gtcgttaacc
    42901 acgaaggctt tccggctacc gaggctcgtt tcctcatcct ggatgatgga tctccgatac
    42961 ataccgcatg gccggaggac aaaatccttg tattggaaga cgatcaaaaa gtttccgcag
    43021 agtatgaccc ctacaagcaa cctatagaca ccacagtagt ctatcactat acagaatata
    43081 cagacataga aagtggggga tcgcacaggg aggtaatgga tgagttagta gcaaatttga
    43141 aacaggaaac ccgcttgcgt tccctatgcc tcacaatggt atccgtcagg gagccggttc
    43201 cttgtcggcc accgggcacc ggggaaactt tgggcgagtc atttaaatcg aggactgatt
    43261 attcgagaac ggaagttacc aattcgatcc gtcctattag ttcccagttg atatgggaca
    43321 gtatcattgc cgagcgcgtc cctaatcgtc ctcatttggt catttgggtg gtcgatgatg
    43381 atatattaac ccatgatgat atgcccggtg acttgctgcc tgcgacttca gaccaactta
    43441 ctcgcctgcg tgacgccgat atctccctga tcgttctcga tctgacaccg gcggacgatc
    43501 ttttgcgaga tgaacagtgg aagggcatgc taaaaacctt taacatcctc caccttaaat
    43561 atgcgggaac gaactatcta ttgaacgaca ccaagtgtgg tcctgcctgg atctgtgtaa
    43621 aacaggaact ggatccaaag cgaccgatgg tccacgtcct gcgatataat tcaagccttt
    43681 ttgttgatgg cgcccaacac agcctccgtg ttcaattgca atccgggaat cttggcggca
    43741 acgccggaac cgcatcttca ataattgctc atgaaagaac cgactttatc atcgcgccac
    43801 cttatcaaaa cgatcacatc agcctacgtt cagaagtacg attttttagc agagcgttca
    43861 atgcgattgc catgatcgtt ttgatgttgg tgctggtctt gatcaagcgt ccccaggatg
    43921 gatcggtcta gaacggattt ggaagaggca tcttatgctt gggcgcatca tcggtctcgt
    43981 gctcgtagta gttcttattt tgttattcgt cgtactatcg ttgtttatgc gttccaatga
    44041 aaatcctcct tcttcaacgg cggagcctac ggaactcgtt gatgagcctt caagcactcc
    44101 gaatgaggga ccagggccca ttcctctcac ccttacgcca tcagccactc ctttcattcc
    44161 tgcgccgtca gccactcctt tcattcctac gccgtcaccc agcgcaactc cagaggccga
    44221 tctcacccta accttcgttg ccataccctc cgaacagcct cctgtcatat taacgcctgc
    44281 ccccactgaa acgccacaac ccacagtgac ggtaacaacc accccgaccc ccatcacgaa
    44341 catgccactt ccccccttgc ctccccttcc gccggggaat atatgcatgc aagatgttca
    44401 ggacatcagc tcaccgacca atggatttga gtatagcgat gatcggactt atatcgaact
    44461 tcacggatcc gcggcctggc ccgccgatcg ggaaggacag tttctcaaat acgaggtgtt
    44521 ttattatgat ccgggcaatc agaaacacga cctggctgaa gtctggacct ccgtttccgg
    44581 cggcttgcta caccggcagg cgtttggccc cctggtccat cagtacggcg aacccggatg
    44641 gtacaccttt ggtgtgcttg ggataagaac cgacagcaat cacttcccgc tgggttctta
    44701 caatgagggt tgctttgtcc gcgtctatct gccgtggccg cgttgagtct ctcttcgggg
    44761 aacacgtctt ccgtgccccc cacttcgccg cgagactagc tcccacccct accccaaaac
    44821 cgtccgcgga caggattgcg gggcgagggg gcagcgttta tcgtggcggc atggagaaat
    44881 tacatgcccc cgtccccgga gaagtctggc tgttgcacac gccgccgcgc caggctatgt
    44941 tcgccctcgt ggcccgcatg gccacgatcg ggccggtgct ggcgctcgac gccgccgacg
    45001 aattcgacgc ccatcaagtc gcccgcctca tccgccgccg agcgccgcga cggttcaacc
    45061 gctcgctcgg ccatgttcgc gtcggtcagg ccggcacctg ctatcaggtg ctggccctct
    45121 tcaagcagct gccggccacg tccatgcccc acatcatctt cgacctgccg gccaccttct
    45181 gcgacgagag cgtcccgctg ccggagagcc ggcgcctgct gcagatcgct ctcaaccacg
    45241 tcgaccggct gcggcgggag gcgccgatca tcatcagcgt gcggccgacg ccgggcggcc
    45301 gtcgcgccga actgctgcag gcggtcatcg atcgcgccga tcatcttttt gtctgggaat
    45361 cgcccggcgt gcccgtgccg gtgaagttgt tttgaaatga cgagtgacga gtggcaagtg
    45421 acgctcttca ttcataattc ataattcgta at
//
LOCUS       DAOMQG010000003        31255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_650_length_31255_cov_11.104423, whole genome shotgun sequence.
ACCESSION   DAOMQG010000003 DAOMQG010000000
VERSION     DAOMQG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 31255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31255
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_650_length_31255_cov_11.104423"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLC06_00410"
     CDS             <1..447
                     /locus_tag="PLC06_00410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO88938.1"
                     /translation="TGSPEYENMFSRLASYHRDKMAAYIGFHAGLAPLIYAGSDMFLM
                     PSRFEPCGLGQMIAMRYGSVPVVRATGGLADTVHNGITGFSFYEFSAGALWDAIQRAT
                     FTYNTDKESWRQIQLNGMNTDFSWRRSAQGYQQLYEWAVAQKRYRG"
     gene            517..3375
                     /locus_tag="PLC06_00415"
     CDS             517..3375
                     /locus_tag="PLC06_00415"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRO88939.1"
                     /translation="MPDDNSTRFTLINPVTTGFYINDPFNSPRSYANGRHEGVDLRAV
                     SGGRPSEIIAAQRGIIERIRPGDSGYGNYVRIRHDWGDGTTWVTWYAHMSSFNPTIQE
                     GIMVEAGRRLGLAGTTGNSTGVHLHLTLQHLGHGLKGYVIPDVVDPTRYFSDVTVPLI
                     DEVSYVADVTIPDGSALVANTPFIKTWRVRNTGTSTWAGFTLEHFSDDRLDGPESVVL
                     PSLEPDETGEVSVELVAPNAPGRYRSTWKARNGRGRLFAFEMFADIVVTPVSRRDDAV
                     LVANIAPADGEDIEINRKVLKTWRVRNTGDTTWDKTYTLVNESNPLGADVSIRLPATK
                     PGATADLSVVLTTPAKPGIFHSTWQLKAPDGRAFGPSLIAELRVISLPGKLRDNAEFV
                     TDVTIPDGARLSPGARFTKTWKVRNAGTSTWGDGYLLAFLGDSRMEGPEAVAVPLTKP
                     GESAELSVELKAPDSPGHHRSAWQLKSPAGEPFGDILRVEIEVISLGAVDNARFVTDE
                     TFPDGDLVTAGKRFIKTWRIRNTGSSSWVAGFALAFAGDNQMSGPDSMPLPAALPGEE
                     VVVSVSLVAPLTPGSHRSIWRPRNPNGMLFGDLLYADIRVPITSTPGSPALEDAQLES
                     HVSYPDGTEVRAGATFEKIWAIRNTGSTPWSEGYRLVRVGGTDMGAVPYIPVGRTGSQ
                     EVTQITVSMTAPPEPGRYISRWRMVNPRGEQFGSTFFAAIVAVESPTKYDMLPYLRGD
                     GRLYEMKHIFEMPNGLQIGQQRVQTQRDGTRFYQTKNSEWEEMWADSRFIYRGTDTSP
                     GSGNFYTLMDGERYGTVWIPRFMAVGQTYRRSVTVVSRRKGNCVMNSHLSGRHITWAR
                     LEAMHSSLALPDVEGRPGRGIKMRDVIVLAAYNEVQGRPADRPFERYYYAMDFGLVMW
                     EGIDTDHRGISFLVQVHNPGARPDNVRERIPCLENLRP"
     gene            3537..>4341
                     /gene="malQ"
                     /locus_tag="PLC06_00420"
     CDS             3537..>4341
                     /gene="malQ"
                     /locus_tag="PLC06_00420"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRO88940.1"
                     /translation="MSFERSAGVLLHPTSLPSRYGIGDLGAGAYHFVDFLTSAKQRLW
                     QILPLGPTGYGDSPYQCFSAFAGNPLLISPDRLVADGYLPAKAIEVVPDFPTSKVDFG
                     PVIEYKRALMRQSHEYFKANGDKSQHEAYNQFCRNTAYWLDDFALFMALKEYHKDQDG
                     GVWNTWPKAIAKRQVRAMKQWTKQLAEEIDLHKFQQFLFFKQWLELKAYANGRGIRII
                     GDIPIFVAYDSADVWAHPELFYLDKNGSPSVVAGVPPDYFSATGQRWGNP"
     assembly_gap    4342..4441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4442..5266
                     /locus_tag="PLC06_00425"
     CDS             <4442..5266
                     /locus_tag="PLC06_00425"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRO88941.1"
                     /translation="AGVPPDYFSATGQRWGNPLYRWDVIAANNYAWWVQRIHMNLVQA
                     DIVRIDHFRGFEAYWEIPATEPTAVIGRWVKGPDADFFDAIRAKLGDLPIIAEDLGVI
                     TPEVVALRDRYNFPGMKILQFAFGGERNSTFLPYTYDKNAVVYTGTHDNETTLGWYLN
                     ATEDERDHVRRYTVSSGRDIVWDLIRLAYASVADLAIIPMQDFFVLGNEARMNYPGRE
                     GGWWSWRYTREMFSARAAGIAMGLAELARLYGRIPEDTIAPSEAEVVEHGATAASE"
     gene            5241..6032
                     /locus_tag="PLC06_00430"
     CDS             5241..6032
                     /locus_tag="PLC06_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HRO88942.1"
                     /translation="MEQRPQASDPAAYAEINSLLNNLLKDAQAILGDQLIGFYIDGSL
                     AFGGFDPATSDIDFIMATAGDLPDEIVGQLAAIHRRITLSNRAYADQLEGSYIPLSAL
                     RRYNPTDATHPNIERGPNEVLRPKYHHSDWVIHRYITREHGITLHGPSPKTLIDPVTP
                     DDLRWATIEALRSWWATPEAAESIRHSHPGYWGYVTQTMCRALYTLEHGEVIDKATAC
                     RWALDTCDEQWRPLIENSRSRNEAIEVEHLLQYISYVHERAGNVL"
     gene            6112..6618
                     /locus_tag="PLC06_00435"
     CDS             6112..6618
                     /locus_tag="PLC06_00435"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HRO88943.1"
                     /translation="MRRRSNIRSTRRTVRRMHRRRVRMRRRRILLTGGLVAIAVAGTA
                     SAVKMSQRDAQRIEAHTGKKPEDLTEEQLEAAINDLEIDTQELTDADVAAIEAAGPEP
                     AAESASAAPPPAAPAAPAPPAAAPAAPAAPAQPDYITELKRLAELRDMGIITAEDFET
                     KKNQLLGL"
     gene            6822..8210
                     /locus_tag="PLC06_00440"
     CDS             6822..8210
                     /locus_tag="PLC06_00440"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRO88944.1"
                     /translation="MSELSNAARQYSREHAEQFRGELHEMLRIPSLSADPAHAGDIRR
                     MAEWLANHMRELGLDNVAVMPTAGHPVVYGEWLGAGAGQPTMLVYGHYDVVPALMEDG
                     WHTDPFEPVEKDGRIYARGATDDKGQLFIHVKALESWLKTAGRAPINVKFLLEGEEEV
                     SSPNLVPFIKENLDLLSADLCVISDSSMRSIDEPAILHSLRGMTYVEMEVHGPTDDLH
                     SGLWGGAAHNPALALVEILGKLYNPDNTIAVPGFYDDVVPLTDEERAMIAKTDLTEEQ
                     YKASTGVPAIWGDAKYNIRERIAARPTLDINGMWSGWTGPGPKTIIPSKASAKFSSRL
                     VGNQDPNKIFELIKSYIESITPPTVTVDVRLLTTGKAALFPFDTPEMKAASRAYEEGW
                     GATPIFTRGGGSIPVVAEIADLMGIPVVMMGYGLDDDGLHSPNERFSIEMFHRGIETA
                     IVYLEELARPGN"
     gene            8262..9725
                     /locus_tag="PLC06_00445"
     CDS             8262..9725
                     /locus_tag="PLC06_00445"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRO88945.1"
                     /translation="MKITVKSGNVLHQESDLSVLATFEDIDLPGDIAGLLEPNDYRGR
                     TGQTVLLYPRGAVAPRRLLLVGLGKRESATAENVRRATAEAVRRARELQVASFNIGSA
                     GNLPFDAESAGQALAEGLSLGAYRYWKYRTGLTAEQTFAVESATAYVNDERAAGVAAG
                     VATGLVVARGVAFARDLVNSPGYHMTPARMGKEALDLGERLGLKVTVLDKAQLIEGGF
                     GGIVAVGQGSANDPRFIVMEYGQAAEGTPTICLVGKGLTFDSGGLSIKPAEAMDTMKS
                     DMAGSAAVFGAMQVVAELGLPLHVVGLVSSAENMPSAMAYRPGDVVTTLSGKTIEVLN
                     TDAEGRIILSDALYYAQRYNPDAIIELSTLTGAIVIALGPHAIGMMGTDQALADKVSS
                     AGDASGERVWQLPMWPEYHEMIKSEIADVKNIGGRAAGSITAGAFLAAFTGDYPFVHL
                     DIAGTAWNDRPAKSYDSHGGTGVGVRLLTEYLRDYTR"
     gene            9838..11475
                     /locus_tag="PLC06_00450"
     CDS             9838..11475
                     /locus_tag="PLC06_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRO88946.1"
                     /translation="MAKAAAKQPKKSGGWLGTVERIGNKVPHPVLMFLYLIIGVILLS
                     AVLSWMGVSVTEEIAVPIPSESVPDFYEDTTEPSQAFPTSPYDTDYEIQEQTIAINSL
                     LTVEGIRFIFTSFVPNFAGFSVVAVVFIAMMGAGVAEESGLMTALIHKLVEVSPRWAL
                     SFIIIFVGVLSSVATDAGYLILIPLGAAAFLSVKRHPLVGVAAAYGGVSAIFAINILI
                     TPLDAMLTEITNEAIALAGGEPITIVSNLYFQVVMSILLSIVAAFITDRIVEPRMGKY
                     VPEAGAKLAEDVKLDKAAQSRGMRFAGLGALAVVVIVFLLTVFPGAPLRHPETGGIIG
                     ATPFMDSLLFIITLIFLMAGIGYGYGAKTFSGSNDVIKAITKTFASLAGLVFMLLMIA
                     QFIAYFNFSNMPSVIAVTLANWLEQANIGAVPLLIGFIIVIMLLDIIIPGSMPKWAIF
                     APIFVPLFMRLGTAPQTVLAAYRIGDSPMNVITPLMVYLPFIITVMQRYQKDTGIGTV
                     IALMLPYTLIIAVAWIILFILWYVIGIPLGPGYPVGV"
     gene            11533..12774
                     /gene="pepT"
                     /locus_tag="PLC06_00455"
     CDS             11533..12774
                     /gene="pepT"
                     /locus_tag="PLC06_00455"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRO88947.1"
                     /translation="MTMNFEREVEERLLRYVQIDTTADESSPTSPSTERQYDLLNLLV
                     DELNEIGAQDVRLTDYGAVLATIPATIESDAPVIALLAHVDTSPAYSGAGVKPLVHRS
                     YQGGDIPLPDDPEQILTYENSPYLKERIGDDIVTASGTTLLGADDKAGVAIVMAAARH
                     LLNNPDIPHGTIRIGFTPDEEIGRGVHADLPADLGATFAYTLDGAHRGELVYETFSAD
                     RAVVHVQGVPIHPGWAKDKLVNALHLAAKIVDTMPQVTLTPETTEGRQGFIHLYAMSG
                     TAAAAELHFIMRDFEMDELERQGKLIKQVCATIQATEPRAHITCEVLPQYRNMRYWLE
                     NDMRPVEIAVEAYRRANIEHFFLPIRGGTDGSRLTEKGVPTPNLFTGMQNIHGPLEWV
                     SVQDMARATEMCIKLAECWSE"
     gene            12855..14516
                     /locus_tag="PLC06_00460"
     CDS             12855..14516
                     /locus_tag="PLC06_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO88948.1"
                     /translation="MSEKTVHKPSDATPYVTWLYMGIIVLAQAQMAFNVNAIPVSIGP
                     IVEELNIPATGVGTALVVYSLFVAAFVMLGAKIGKIFGERLVFQVTALLHGLSMAVMA
                     VSQDATTMNIAQALAGIAAAALVPTLVVLIAANYNGAQKAQALGILAATPALSGALAF
                     FVAGFLGTYLSWRYSFGMLTFISIIVFLLSFRLKPIPRQSGVKIDFIGVSLSAIAVIL
                     ISFGFNNLNSWGIVLASTNAPFSLVGLSPALLFLVFGLMLFQAFFAWSHQRTELGREP
                     LLSLEVIDSPKERATIVSLLIIGALGPAVNFLIPLFIQIVQGRTTMQTAVAVVPYTLA
                     IATSAIFIVRFYDRFTPRRIAVVAFTLVAAGLALLSFVVGNNWGTAMVIFGLVMVGLG
                     EGSLLTLLFNVMVTSAPKSLAGDVGALRGVANNLSTALGTAFAGVVAVSLLSMFVMGG
                     IDQSSLPHSLVRQVNLDNVNFVSNGHLDEVMAQTTATPEQVTQAVQINETARLRALRA
                     SFLILSALALLAIFPAMGLPDYSFGDEPPEEPAAKPKRRSRKKPA"
     gene            complement(14579..15589)
                     /gene="glsA"
                     /locus_tag="PLC06_00465"
     CDS             complement(14579..15589)
                     /gene="glsA"
                     /locus_tag="PLC06_00465"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HRO88949.1"
                     /translation="MKETVNPQAKKKRKKYVSTGHLPPTEAVQTIVDEAYARYKDNDE
                     GQNASHYPALARVPRDLFGVCVINTHGEVMAAGDADYEFTIMSVSKPFTFGLVCEAIG
                     AKEARDKLGVNATGLPFNSIMAIELNPSRLTNPMVNSGAIAAVTLVPGETAEAKWRFI
                     HEGLSRFAGRTLNLNDEVYASASATNHRNRAIARIMYDYGRLYFDPVEAADIYTRQCS
                     LNVTARDLAIMATTLANGGINPVTGEQVLDAAHCRRVLAVMTTAGMYETTGDWEFDVG
                     LPGKSGVSGGIITVSPGKGGMGVFAPPLDSAGNSVKGQLVARFLSDYLGMNVFASAPE
                     SQ"
     gene            15943..16674
                     /locus_tag="PLC06_00470"
     CDS             15943..16674
                     /locus_tag="PLC06_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HRO88950.1"
                     /translation="MGARRVNRTRLRPKPDKPKEGFMAHLLDPIDRLAETIYSILVLL
                     TFTLAFRLFHLSPGEIVSSEYVNELLIGASLAILAWGIIDGIVYVLTEVFERGEKHRI
                     LLYIESADSPAEAVEAIADELDFILEPITSESQRRHLYADILEHLHDSTPQPVHLKRE
                     DLTGGLASVLVAVLAVLPSLAPFILFRNDHNLAIRASNVVSFAVLFYSGYEWGKYTGS
                     NPWKTGLLLLTVGVLLVAIAIPFGG"
     gene            complement(16706..18220)
                     /locus_tag="PLC06_00475"
     CDS             complement(16706..18220)
                     /locus_tag="PLC06_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO88951.1"
                     /translation="MEGILIPSIFYIRGEHMQKYLSTMLKAVLTVVGGVSFLLSCTNE
                     VPGVPSVATIINETIITPPPETVSPSATGSVEPAVITSDLATSPIPIPTITVPAVRAT
                     NTPIPSPTVITPEIVGLDDTLIFYTTLVDMRTSPNRPPGVSTLDDYYAFKTWPPLPFV
                     DPVIFERLYGEREMIEPIGMFFLNFRLRPSPDGRYMLVPGLSHNSNNDPEIAGTWLLD
                     LEVGEARKLLPDGVIATWNPAGDAITYVSEGTLYTLDIATGSEPKSLFEDEGLWELYA
                     YWSPDGRWIAVLTSVNPELIDPENPKYAAAYWIVPTNGDQARTLTVQETFAIEYTTSD
                     ISWSPDGHYLLVRNKVFDLEGNLLSPDYPGGVSWLPNNSDLLVNSREELQIMTIAGEK
                     IAHIADIEYPVTWAWSRDGRRFAYSLGRGASGTDVEVVVYDVERGENQVIGTIPKVNS
                     VGLLRWSADDSHLIIGLDRGNQDEIWVVAAQSGSMAELILENALLIEVVPYPVR"
     gene            complement(18245..19441)
                     /locus_tag="PLC06_00480"
     CDS             complement(18245..19441)
                     /locus_tag="PLC06_00480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRO88952.1"
                     /translation="MRPRFPHRSISATIVAMLLIVAVVVIAQAQNSAPKDDVKWVTYV
                     DPRFHFSVKYPSDWQVIPRDDSDPRALSGLLTFVPVEVSQFTGSQNHQGSGPQITIGL
                     YLAEIEPSQSLSEWTERYESLSIASDEGSVQRQQRRVFEVGDVTAIHEEGISPVTTYQ
                     FTNLARGRIVWFIWTNIPSGPQSADSKVYRKMVRTFRVSKEMPSTLSEVYGSDFKSLD
                     LNDNSRTPEVTDEALTSDDIEVTNLTSSWLSPVLKKSDGKFWNVQCGSDFHTGIIRWA
                     ADIGVGVGNKVYSARVGVVTWAGQHNSSYGNLIKIKASGGKEAFYAHLQSIYVQPGAH
                     VSSPSQIALSGNSGGVDAHLHFHVQNGSDGENLVGMSGFYPKSNWPTDNDFPIDQDES
                     CANMGR"
     gene            19620..19691
                     /locus_tag="PLC06_00485"
     tRNA            19620..19691
                     /locus_tag="PLC06_00485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19653..19655,aa:Arg,seq:ccg)
     gene            19902..21539
                     /locus_tag="PLC06_00490"
     CDS             19902..21539
                     /locus_tag="PLC06_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO88953.1"
                     /translation="MTGAGLDSETRDMILDTLRKYAERKLTTDYLLQLDHDDRFPEEV
                     LKELYDPMQLGLHLLFIPEEFNGLGGGAYDIYRVSELMAAIDLGVATGVLATFLGSDP
                     IRVGGTPEQKAHWMGRIADEALLMAYGATEPQAGSDLAAMSTKAVPIQENGSVTGYKI
                     SGRKQWISNGGVADLYTILALAPGGPSWFVVERGAEGLSHGKGEDKHGIRASNTAALF
                     LEDVHVPTERLIGGVEGQGLAQAQAVFGYTRLMVGAFGLGAGWEALRRAIHYAHERIQ
                     GGAPLSEKQGYTHKLIVPNASRLEAARTYIEWVAERLDSGEEGLQTEGAVAKYMATEA
                     GNKAAEDAIQALGGYGYTKEYMVEKIKRDVRITCIYEGTSEIMEWTIARDRWQQHLKT
                     QGAFYNDWAARLDKLHAAEPDNGANFAALAMRALAIILERARIDRLTRNQHVLFRLGE
                     LIAWAETAAVFAERVTSHPTEAIHLDVPTRQALARIHGRNAALKVATDGLQWAIGAGQ
                     TDPNLAGSLNLPGVYAAQAGQIDDMNFARDQLNRAFS"
     gene            21639..>27388
                     /locus_tag="PLC06_00495"
     CDS             21639..>27388
                     /locus_tag="PLC06_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRO88954.1"
                     /translation="MDSADRAIAIVGVGAILPDAPSAAAFWNNIVGKRYSITETPPER
                     WSITDYYDSDPKTPDKTYSKIGGWVRGFEFDWKRFRMPPKVAASMDEGQQWAVTIAAD
                     ALADYGYPDRPLDTERTGVILGTAMGGELHYVTSQRIVFPEYVRWLRSTPEFAKLSPA
                     TQEALIEQFHAALDQDVPPITEDSMPGELPNIVSGRVANMLNLRGPNFITDAACASSF
                     AAVEAAIQMLNENKVDAVLTGGVDRNMGATTFVKFCKIGALSATGTRPFGDGADGFVM
                     GEGAGAFLLKRLSDAERAGDKIYAVIRGVAGSSDGKGKGITAPNPVGQQLAVQRAWES
                     AGLDPATASLVEAHGTSTRVGDVVEVESLSQMFSGAARGSIGLGSAKSNIGHLKAGAG
                     AAGLLKATMALHHKILPPTLHANPTNPNIDFTATPFYPLHDAREWTKSNGTPRRAGVS
                     AYGFGGTNFHLVLEEHMPGALTQRPKLYAGAGEKGSRGAGEKEASSELATRQPAIVPP
                     RGIVALGANSPDELKNKLDELLLRVDAGWTPPNALPRPVDIRAKERLVIDFGDHEELL
                     DRLQKARKVMGFDTPQAWKAMEAQGIFRGRGPKPGKLAFLFPGQGSQYVNMGRELAAV
                     SPTVQATLDEADAIMQPILGKPLTSYLFVDANDKAAVSQANFDLMQTEITQPSMLTLD
                     EAIRRLLADYGFEPDMVMGHSLGEYGALVAAGVLPFAEALEAAAGRGREMAKVKVDDN
                     GWMAAVLAPFEIVEATLKEIDGYVVPANINSTSQAVIGGESKAVEAAIKLFQERGYQA
                     MQLPVSHAFHTRIVAPASAPLRKMLDRMHITPPRLPLVANVTGDFYPASENEIKDLLE
                     KQIASPVQWVKGLQTMHNAGVRTFIEIGPKRALRGFVKDVFGDRDDVVALMTNHPKGG
                     ELPTFNQALCGLYAAGYGAEESAGQRQPAIAATADDGRLTTDVNRTAANGTIAVANEN
                     DMNNNAQPTAASLDTLAQALAQVLQSNGHAASQRVYDRNETPLGSIVISGTGLGLPGI
                     EKPVMDPDNAMRILRGEQFVDLIPERFRNLIVRKQITRLVKGEDGSGRFEAISDPNEV
                     IKLAGRPGSFDLAEEYGVPAELIEALDTTTQLAMAAGIDALREAGIPLVQTYKRTSTG
                     KHLPERWMLPEALRDDTGVIFASAFPGGDRFADEFKRYYINVNRTQQLEMLEDLRNYA
                     TDPATQSEVNRRIDELRDQLEREPYTFDRRFLFRILAMGHSQFAQYIGARGPNTQVNA
                     ACASTAQAIAIAEDWIHSGRCRRVVVIGADNVTGDNLMEWVGAGFLAVGAVTTKDQVA
                     EAALPFDKRRHGTIMGMGACALVVESEDAVRERGMRGIVELLSSETNNSAFHGTRLDV
                     SHIGMVMNSLITAAERRFGLNRYAIAPQTVFMSHETYTPARGGSASAEVMALRHTFGE
                     AANSIIMANTKGFTGHPMGVGIEDVIAVKILEHGIVPPVPNFREVDPDLGPLNLSRGG
                     RYPVQYAIHLAAGFGSQISLTFTRRIPGSLDRVDNPPMYRRWLADVSGYDTAETEVVK
                     RTLRVVAQGAPTRPPMPNRWQPGTGPTMRAAAGGEIAAVELPARMVTPTVDRRPSTAA
                     PVQQPVDNGQKPVEVKPQVVIEQPRPEPVVVAAIPTADSRPPTTVSEAPPAAVVDQPS
                     AVDPVTDKVLAIVAEQTGYPQEMLDLDLDMEADLGIDTVKQAETFLAIRQAFDIPRRD
                     NLKLRDYPTLDAVIGFVRQSRPDLAAAAVKEEPQPAPVTHAPTVPVLATPATDPVTDK
                     VLAIVAEQTGYPQEMLDLDLDMEADLGIDTVKQAETFLAIRQAFDIPRRDDLKLRDYP
                     TLDAVIGFVRQSRPDLAATAAKEEPQPAPVTHAPPVPVLATPAIDPVTDKVLAIVAEQ
                     TGYPQEMLELDLDMEA"
     assembly_gap    27389..27488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27489..30286
                     /locus_tag="PLC06_00500"
     CDS             <27489..30286
                     /locus_tag="PLC06_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO88955.1"
                     /translation="GYPQEMLELDLDMEADLGIDTVKQAETFLAIRQAFDIPRRDDLK
                     LRDYPTLGAVIGFVHQSRPDLAEPTVDHRPPTAVSEASPAAVVGQSSAVTPGYTLADA
                     DAMPRRVPVPSLRPSLDLCKPTGVTLDANSRIVIMMDRGGVGKALVTRLEKLGATPLI
                     INDIPTAEALEAQLKTWLAEGSIQGVYWLPALDVEPEPDEMTLEEWREHNRARVKNLY
                     TTMRTLYDAVKDRGTFLVSGTRLGGLHGYGPEGATAPLGGAVSGFTKAYKRERGDALV
                     KVVDFEPGRKTAPPADALLAETLLDPGVVEVGYYQDNRYTVTLVEEPAANGQPGLALG
                     PDSVYLVTGAAGGITSAIIGDLAAAGGGGSFYLLDLVPEPARDDQQIALFRQGKDALK
                     QHLIATAKTAGEKVTPAQIDKQLMVVERNEAALRAIESVEAAGGKAHYRSVNLLDGLA
                     LTAVVDEIRHQHGRIDVLVHAGGIEISRSLADKDYTQFSLVYDIKADGFFSLMRAAKD
                     MPIGATVAFSSVAGRFGNNGQTDYSAANDLLCKLTSSLRRRRPTTKGIAIDWTAWGEI
                     GMATRGSIPRIMEMAGIDMLPPSAGIPTVRRELVAGSCRGEIVVGGRLGILVDEWDET
                     GGLDTAKANEWLAAQHPPLHMLGEVKAAKLYGGLEVETTLDPNEQPFLYDHAMDGTPL
                     LPGVMGTEAFGQLALALAPGYRVAAVYDEEFHAPFKFYRMESQTLHLSAIATPAGDNN
                     GLIVQASLRSLRDLGKPGLPPQEKLHFTGKVRLVQELGQPVIDFTPPASESLSISAED
                     VYRIYFHGPAYQVLERVDVHDDRAIGLLAAALPSNTNPADAASIMAPRLIELCFQTAG
                     VWDIKTNGTMALPLGLNSVTAYRQPMETNGNRLYAVVTALDGGEAYHAQVVDDSGAVY
                     VDLKGYRTVALPGSVTL"
     gene            30469..31227
                     /locus_tag="PLC06_00505"
     CDS             30469..31227
                     /locus_tag="PLC06_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRO88956.1"
                     /translation="MNATTRSGVITTPQGPLHWLVLHAGQDNLLASDTPPDFLHPTEI
                     RHWQTLKINKRRREWTLGRRAAKELIIEQIYENNGHRLDLARIAIVPHGDGWPMVMLP
                     DQADFPAITLSISHSRDKAFCATTEGADRLLGADIEAIEPRTPAFVEEYFTPLENRFL
                     TVVPADQRMTLINAVWSGKEAALKAIRRGLAEDTRIVSCLPHPAMGDKAQWLPMRIEW
                     TAERTQQPMPPLRGYWRHENGFIMTLAFAADAAP"
BASE COUNT         6445 a         9789 c         8868 g         5953 t
ORIGIN      
        1 accggcagtc ccgaatacga gaatatgttc tcgcggctgg ccagctatca tcgggacaag
       61 atggccgcct atattggctt ccacgccggg ctggccccgc tcatctacgc cgggagtgac
      121 atgttcctga tgccttcccg cttcgagccg tgcgggctgg ggcaaatgat cgccatgcgc
      181 tacggcagcg tgcccgtggt gcgggccacc ggcggtctgg ccgacaccgt ccacaacggc
      241 atcaccggct tctcttttta tgagttcagc gccggcgcgc tatgggatgc catccagcgt
      301 gccaccttca cttataacac cgataaagaa agctggcgcc agatccagtt gaacggcatg
      361 aacaccgact tctcctggcg gcgctccgct cagggctacc aacaactcta cgagtgggct
      421 gtggcccaaa aacgttatcg cggctaacgt gccggtcatt aatttgtcga acagatccaa
      481 caaggtgggc tgtcgtaatt caggtaggaa gtatcaatgc cggacgacaa ctcgacgcgc
      541 ttcacactaa tcaacccggt cacaaccggt ttctacatca acgatccgtt caacagcccg
      601 cgctcctacg ccaacggccg tcatgaaggc gtggacctgc gggctgtttc cggcggccgg
      661 ccgtcggaga tcatcgccgc gcagcgcggc atcatcgagc gcattcggcc cggcgacagc
      721 ggatacggca actatgtgcg catccgccac gattggggcg acggcacaac ctgggtcacc
      781 tggtacgccc acatgtcttc tttcaaccca acgattcagg aaggcattat ggtggaagca
      841 gggcgacgcc tgggtctggc cgggacgacc ggcaactcta ccggcgttca tttgcacctg
      901 acgctccagc acctgggtca tggcttgaag gggtatgtaa tccccgacgt agtcgatccc
      961 actcgatact tcagcgatgt gacggttccg ctgatcgacg aggtgagcta tgtcgccgac
     1021 gtcaccatcc ccgatggctc cgcgctggta gccaacacgc cgtttatcaa gacgtggcgc
     1081 gtgcgcaaca ccggcacgtc cacgtgggcc ggcttcacgc tggagcattt tagcgatgac
     1141 cggctcgacg ggccggagag cgtggtgctg ccgtcgcttg agccggatga aacgggcgag
     1201 gtcagcgtcg agcttgtcgc ccccaacgcg cccggccgct atcgcagcac ctggaaggcg
     1261 cgcaacggcc gcggccgtct cttcgcattt gagatgttcg ccgacattgt agtgacgcct
     1321 gtctcccgtc gcgatgatgc cgtgcttgtg gccaacatcg cgccggccga tggcgaggac
     1381 atcgagatca atcgcaaagt cctgaagacc tggcgcgtgc gcaacaccgg cgacaccacc
     1441 tgggacaaga cctacacctt ggtcaacgag agcaaccccc tgggtgccga tgtcagtatc
     1501 agactgccgg cgaccaaacc gggcgcgacg gccgacctgt ccgtcgtgct gacgacaccg
     1561 gccaagccgg gtatcttcca ctctacctgg caactaaaag ccccggacgg ccgcgccttc
     1621 ggcccttcat tgatcgctga gttacgggtg attagcctgc ccggcaaatt acgcgataac
     1681 gccgaattcg tcaccgacgt cactattccc gacggcgcgc gactgtctcc gggcgcacgc
     1741 ttcaccaaga cctggaaagt tcgcaacgcc ggaacctcga cctggggtga tggctatttg
     1801 ctggccttcc tcggcgatag ccggatggaa ggccccgaag cggtggccgt ccccctgacc
     1861 aaaccgggcg aatcggccga actgtccgtc gaactcaagg cgccggattc ccctggccat
     1921 caccgttctg cgtggcaatt aaaatcgcct gccggcgagc ctttcggcga tattctgcgg
     1981 gtggagatcg aggtaatctc ccttggtgct gtggacaatg cgcgcttcgt caccgacgag
     2041 acctttcccg acggcgattt ggtgacagcc ggcaaacgct tcatcaaaac atggcgcatc
     2101 cgcaataccg gctcttcttc gtgggtggcg ggcttcgctc tggccttcgc cggggacaat
     2161 caaatgagcg gccccgacag catgccgttg ccggccgcgc tccccggtga ggaagtcgtg
     2221 gtgagcgttt cgctcgtcgc gcccctgact ccgggcagcc atcgcagtat ctggcggcca
     2281 cgaaatccca acggcatgct cttcggagat ctgctctacg cggacattcg cgtgcccatc
     2341 acctcgacgc caggttctcc ggctctggaa gacgcccaac tggagagcca cgtcagctat
     2401 cccgacggca ccgaggtgcg ggcaggcgca accttcgaaa aaatctgggc cattcggaat
     2461 acaggctcga cgccatggag cgagggctac cgtctggttc gggtgggcgg caccgatatg
     2521 ggcgctgtcc cttatatccc cgtcggtcgc acaggctcgc aggaagtgac ccaaataacg
     2581 gtcagcatga ctgcgccgcc ggagccgggc cgctacatca gccgttggcg gatggttaac
     2641 ccgcgcggcg aacagttcgg ttcaactttc tttgccgcca tcgtcgccgt cgagtcgccg
     2701 acgaagtacg acatgctccc ctatctgcgc ggggacggcc gtctctacga gatgaagcac
     2761 atcttcgaaa tgcccaacgg ccttcagatc ggccagcagc gcgttcagac ccagcgcgac
     2821 ggcacacgtt tttatcagac caagaacagc gaatgggaag agatgtgggc cgatagccga
     2881 ttcatctatc gcgggacgga tacttcgccg gggtcgggca acttctatac actaatggac
     2941 ggtgagcgct atgggacggt ctggattccg cgattcatgg ctgtcggcca gacttaccgc
     3001 cggagcgtca ccgtcgtgag ccggcgcaag ggtaattgtg tcatgaattc gcacctgtcg
     3061 gggcgacaca tcacctgggc gcgtctcgag gccatgcaca gcagcctggc gctgcccgat
     3121 gttgaaggtc gaccaggtcg gggcatcaaa atgcgggacg tgatcgtttt ggcggcgtat
     3181 aatgaggtgc agggtcgacc ggccgaccga cctttcgagc gatattatta cgccatggat
     3241 tttggcctcg tcatgtggga aggcatcgat acggatcatc gcggcatctc gtttctcgtt
     3301 caggtgcata atcccggcgc tcgccccgac aatgtgcgcg agaggattcc gtgcctggag
     3361 aatctgcgac catagcattt tttgtggctg attggcgtta tttgattgcg cttttgtggt
     3421 caatgctcaa tattactgtt cgtagtcggc aactattgag cgtttacaaa tgaataacag
     3481 aatttaattc attcatgcag cggtgcacat acctaattgc acaggagaac tcatccatga
     3541 gctttgaacg atccgcggga gttttgctcc acccgaccag tttgccgagt cgttacggca
     3601 tcggcgatct gggcgccggc gcataccatt tcgtcgactt tctgaccagc gccaaacagc
     3661 gtctgtggca gatattgccg cttggcccca ccggctacgg cgactccccc tatcaatgct
     3721 tttcggcatt tgcaggaaac ccgctgctca tcagccccga ccgtctggtg gccgacggct
     3781 acttgccggc caaggcgatc gaggttgtgc ctgacttccc cacgagcaaa gtcgatttcg
     3841 ggccggtcat tgagtacaaa cgcgcgctca tgcgccaatc gcacgaatac ttcaaggcaa
     3901 acggggacaa atctcaacac gaggcctata accaattctg ccgcaatacc gcctactggc
     3961 tcgatgattt cgcgctcttc atggcgctga aggagtacca caaggatcag gatggcggcg
     4021 tctggaacac ctggcccaag gccatcgcca agcggcaagt tcgggccatg aaacaatgga
     4081 cgaaacaact ggccgaggag atcgacctgc acaagttcca gcaattcctg ttcttcaaac
     4141 aatggctcga actgaaagcc tatgccaacg gccgcggtat ccgcatcatc ggcgatatcc
     4201 ccatcttcgt cgcctacgac agcgccgacg tgtgggccca ccccgagctg ttctacctcg
     4261 ataaaaacgg ctcaccctcg gtcgtggccg gtgtgccgcc cgattatttc agcgcgaccg
     4321 gccagcgctg gggcaacccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 ngccggtgtg ccgcccgatt atttcagcgc gaccggccag cgctggggca acccgctcta
     4501 tcgctgggac gttatcgccg ccaacaacta cgcctggtgg gtgcagcgca tccatatgaa
     4561 cctggtgcag gccgatatcg tccgcatcga tcacttccgc ggcttcgagg cctattggga
     4621 gattccggcg acggaaccga cggcggtcat cggccgttgg gtcaaggggc cggacgccga
     4681 cttcttcgat gccatccggg ccaagctggg cgacctgccg atcatcgccg aagacctggg
     4741 cgtcatcacg ccggaagttg tggccctgcg cgaccgctac aacttccccg gcatgaaaat
     4801 cctccagttt gccttcggcg gcgagcgaaa cagcaccttc ctgccctata cctacgacaa
     4861 aaacgcggtc gtctacacgg gcacccacga caatgagacg acgctgggct ggtatctcaa
     4921 cgccaccgaa gacgagcgcg accacgtgcg gcgctacacc gtatcttccg gccgcgacat
     4981 cgtctgggac ctgattcggc ttgcttatgc ctcggttgcc gatttggcca tcatccctat
     5041 gcaggatttc ttcgtgctgg gcaacgaggc gcgcatgaat taccccggcc gcgagggcgg
     5101 ctggtggagc tggcgctata cgcgggagat gttcagcgct cgcgccgccg ggatcgccat
     5161 ggggttggcc gaactggcgc ggctctatgg gcgcattccg gaagatacga tagccccaag
     5221 cgaggcagag gtcgtggagc atggagcaac ggccgcaagc gagtgatcct gccgcctatg
     5281 cagagatcaa ctcgctgctc aacaacctgt tgaaggatgc gcaagccata ctcggcgacc
     5341 aactgatcgg cttctacatc gacggttcgc tggccttcgg tggtttcgat ccggcgacca
     5401 gcgatattga cttcattatg gccacggccg gcgatctgcc tgacgagatc gtgggccaac
     5461 tggccgccat acaccggcgt atcaccctca gcaaccgagc ttatgccgac caactggagg
     5521 gcagctacat ccctctgtcc gctctgcgcc gctataaccc gaccgatgcg actcacccca
     5581 acatcgaacg tggcccgaac gaggtgctgc ggccgaaata ccatcacagc gactgggtga
     5641 ttcatcgcta tattacgcgc gaacacggca ttactctgca tggcccatcg ccaaagacac
     5701 taatcgatcc cgtcacaccc gacgatttgc gttgggctac gattgaggct ctgcgttcgt
     5761 ggtgggcgac accggaagcg gccgaatcga ttcgccacag ccaccccggc tattggggtt
     5821 acgtgaccca gacgatgtgt cgggcactct acacgttgga gcacggcgag gtgatcgaca
     5881 aggcgaccgc gtgtcgctgg gcactggata cttgtgatga acaatggcgg ccgcttatcg
     5941 agaactcaag gtcccggaac gaggccatag aggtcgagca tttattgcaa tacatcagct
     6001 acgttcacga aagagctggc aacgttctct agacgtttcg cgtataagca tttatactct
     6061 caatatattt cattgaaaga agatactggt taattcacca aggagctacc tatgagacgt
     6121 cgaagcaata ttcgctctac ccgccgcaca gttcggcgca tgcaccggcg gcgagtgcgc
     6181 atgcgccgcc gccgcattct gctgacgggc ggcctggtcg ccatcgccgt ggccggcacc
     6241 gccagcgccg tcaagatgag ccaacgcgat gcccagcgca tcgaggccca caccggtaaa
     6301 aagccggaag acctgaccga agaacaactg gaagcggcga tcaacgatct ggagatcgat
     6361 acccaggagt tgaccgatgc cgacgtagcc gccattgaag cggccggccc cgaacccgcg
     6421 gccgagagtg cgtcagccgc accgccgccg gcggcgcccg ctgctccagc accgcccgcc
     6481 gccgctccgg cagcacccgc cgcccctgcc caaccggact acatcaccga gttaaagcgg
     6541 ctggccgagt tgcgcgatat gggcatcatc acggccgagg atttcgagac caagaagaat
     6601 caactgttgg ggctttagcc ttgccttatc aagccggctg ttcggtcaga cgaacagccg
     6661 gcttcagtcg ttgttaccgg tggcgatttg gggctgggga gttctgtatc gactcaatgt
     6721 gccggtttgc cgatgttccc cacccttcta gtaatctttg atatttaaaa taaaatagcg
     6781 tcagtcgcag gcaaaataga tctctgtttc aggagcaagc tatgtctgaa ttaagtaacg
     6841 cagcgcgcca atactctcgt gaacacgcgg aacagttccg cggagagctt cacgagatgt
     6901 tgcgcatccc cagcctcagc gccgaccccg cccacgccgg ggacatccgc cgcatggcgg
     6961 agtggctggc caatcacatg cgcgagttgg ggctggacaa tgtcgccgtc atgcccacgg
     7021 ccgggcatcc cgtcgtttac ggcgaatggc tgggcgcagg agccggccag cccacgatgc
     7081 tcgtctatgg ccattacgac gtcgtgcccg ccctgatgga agacggctgg cacacggatc
     7141 cgttcgaacc ggtggagaag gacggccgaa tctacgcccg tggcgcgacc gacgacaagg
     7201 ggcaactatt catccacgtc aaagcccttg agtcctggct gaaaacggcg ggtcgcgcgc
     7261 cgatcaacgt caaattcctg ctcgaaggcg aagaggaagt ctcgtcgccc aacctcgtcc
     7321 ctttcatcaa ggaaaatctg gatcttctga gcgccgatct gtgcgtcatc agcgactctt
     7381 ccatgcgcag catcgacgaa ccggctatcc tccacagctt gcgcggcatg acctatgtcg
     7441 agatggaagt ccacggcccg accgacgacc tccacagcgg cctgtggggc ggcgcggccc
     7501 ataaccccgc gctggcgctg gtcgaaatcc tgggcaagct ctataacccc gacaacacca
     7561 tcgccgtgcc cggcttctat gacgacgtcg tgccgctgac ggacgaagaa cgggcgatga
     7621 tcgccaaaac cgacctcacc gaggagcaat acaaggcatc caccggcgtc ccggccatct
     7681 ggggcgacgc caaatacaac atccgcgagc gcatcgccgc ccggccaacg ctggacatca
     7741 acggcatgtg gagcggctgg accggccccg gcccgaagac gatcatcccg tccaaggcat
     7801 cggccaaatt cagctcccgc ctggtgggta atcaggaccc gaacaagata ttcgagctga
     7861 tcaagagcta tatcgagtcc atcacgccgc caacggtgac ggtcgatgtc cgtctgctga
     7921 ccaccggcaa ggccgccctc ttcccgttcg acacgcccga aatgaaggcc gcctcgcgcg
     7981 cctatgagga gggctgggga gccacgccga tcttcacgcg cggcggcggc agcatcccgg
     8041 ttgtagccga gatcgccgac ctgatgggca tcccggtcgt gatgatgggc tacggtctgg
     8101 acgacgacgg cctgcactcg cccaacgagc gcttcagtat cgagatgttc catcgtggca
     8161 tcgagacagc catcgtctac ctggaagagc tggctcggcc gggcaactag cctcagatta
     8221 agggaataca tccgtctcta actcattgag aaggagcaat catgaaaatc acagttaaat
     8281 cgggcaacgt attacatcag gagtccgacc tgagcgtcct cgccactttt gaagatatcg
     8341 atctgccggg cgatatagcg ggattgctgg agccgaacga ctaccgcggc cgaaccggtc
     8401 aaacggtgtt gctctatccc cgtggcgcgg tcgccccgcg ccggctgctg ctggtcggac
     8461 tgggcaagcg cgagagcgcg acggccgaga acgtgcgacg agccacggcc gaagctgtgc
     8521 gtcgggcgcg cgagcttcag gtggcttcgt tcaacatcgg gtccgccggg aacctgccgt
     8581 tcgatgccga atcagccggt caggcgctgg ccgaaggctt gtcgctcggc gcttaccgct
     8641 actggaaata tcgcaccgga ctgacggccg agcagacctt cgccgtcgag agcgccaccg
     8701 cctatgtcaa cgacgagcgg gcggcgggag tcgccgccgg tgtggccaca ggcctggtcg
     8761 tcgcccgcgg cgtggccttc gcccgcgatc tggtcaacag ccccggctac cacatgaccc
     8821 cggctcgcat gggcaaagaa gcgctggacc tcggcgaacg gctcggcctg aaggtcacag
     8881 tgctggacaa ggcgcaatta atcgaaggcg gtttcggcgg catcgtggcc gttggccagg
     8941 gttcggctaa cgatcctcgc ttcatcgtca tggagtatgg tcaggcggcc gagggcacac
     9001 cgacaatctg cctcgtcggc aaggggctga ccttcgattc cggtggcctg tcgatcaaac
     9061 cggccgaggc gatggacacg atgaagtccg acatggccgg ctcagcggcc gtcttcggcg
     9121 caatgcaagt cgtcgccgaa ctggggctgc cactccacgt ggttggattg gtgtcgtcgg
     9181 ccgaaaatat gcccagcgcc atggcctatc ggcccggtga cgtggtaacc accctgagcg
     9241 gcaagacgat cgaggtgctc aacaccgacg ccgaagggcg catcatcctg tccgacgcgc
     9301 tatactacgc ccaacgctac aaccccgacg cgatcatcga gctttcgacg ctgaccggag
     9361 ccatcgtcat cgccctgggg ccgcatgcca tcggcatgat gggcaccgac caggccctgg
     9421 ccgacaaggt gagcagcgcc ggcgacgcga gcggcgagcg cgtgtggcag ttgccgatgt
     9481 ggccggaata tcacgaaatg atcaagagcg aaatcgccga cgtcaagaac atcggcggcc
     9541 gggccgccgg gtctatcact gccggggctt tcctggccgc tttcacgggc gactatccgt
     9601 tcgtccatct cgacatcgcc ggcacagcct ggaacgaccg ccccgccaag tcctacgact
     9661 ctcatggcgg caccggcgtg ggcgtgcgtc tgttgaccga gtatctgcgg gattacaccc
     9721 gctagaacta tgagcctgcc cgcgtccaat cgacatcagg gatagttgat tgggggcggg
     9781 caactcattt tatcgtttga ttattgttca ttgccaagga atcaaaagga gagaatcatg
     9841 gccaaagctg ccgcaaaaca gccgaagaaa tccggcggtt ggttggggac cgtcgagcgt
     9901 atcggcaaca aagtgcctca cccggtattg atgtttttgt atctgattat tggcgtcatc
     9961 ctcctctcgg ccgtcctgag ctggatgggc gtgagcgtca ccgaggagat cgccgtcccg
    10021 atccccagcg aatctgtgcc cgacttctac gaggacacca ccgaaccgag ccaggcattc
    10081 cccacttctc cctacgacac ggactatgaa atacaggaac agacgatcgc cattaacagc
    10141 ctcctgactg tggaaggcat ccgcttcatc ttcacctcgt ttgtgccgaa cttcgccggt
    10201 ttcagtgtcg ttgccgtggt cttcatcgcc atgatgggcg cgggcgtggc cgaagaatcg
    10261 gggttgatga cggcccttat ccataaactg gtcgaggtat cgccccgctg ggcgctgtca
    10321 ttcatcatca tcttcgtcgg cgtcttgtca agcgtggcca cagacgcggg gtacctgatc
    10381 ctcataccat taggcgcggc cgctttcctc agtgtgaagc ggcatccgct cgtcggtgtg
    10441 gccgcggcct acggcggcgt cagcgccatc ttcgccatca atatcctcat caccccactc
    10501 gacgcgatgc tgaccgagat caccaacgaa gcgatcgccc tggccggagg agagccgatc
    10561 accatcgtct ccaatctcta cttccaggtc gtgatgtcga ttcttctgag tatcgtcgcc
    10621 gcgttcatca ccgaccggat cgtcgaaccg cgcatgggca agtatgtacc cgaagcaggt
    10681 gcaaagctgg ctgaagatgt aaaactcgac aaggctgccc agagccgcgg catgcgcttt
    10741 gcggggttag gcgcgctggc cgtcgtcgtc attgtctttc tgctgaccgt cttcccgggc
    10801 gccccgctgc gccatcccga gacgggcggc atcattggcg cgacgccgtt catggacagc
    10861 ttgctgttca tcatcacgct gatcttcctg atggcgggca tcggctacgg ttacggcgcc
    10921 aagaccttca gcggcagcaa cgacgtcatc aaggccatca ccaagacgtt tgccagtctg
    10981 gccggcctgg tcttcatgct gctgatgatc gcccagttca tcgcctactt taacttcagc
    11041 aacatgccca gcgtcatcgc cgtgacactg gcgaactggc tggagcaagc caatatcggc
    11101 gcggtgccgc tgctgatcgg cttcatcatc gtgatcatgc tgctggacat catcatcccc
    11161 ggctcgatgc ccaaatgggc catcttcgcc cctatcttcg tcccgctgtt catgcgactg
    11221 ggcacggccc cgcagacggt actggccgcc taccgcatcg gcgactcgcc catgaacgtc
    11281 atcacgccgt tgatggtcta tctgccgttc atcatcaccg tcatgcagcg ctatcaaaag
    11341 gatacgggca tcggcacggt catcgccctg atgctgccct acaccctgat catcgccgtg
    11401 gcgtggatca tcctgttcat cctgtggtac gtcatcggta tcccgttagg cccgggctat
    11461 ccggtaggcg tctgataatt gttactttgt agagacgggt cggggacccg tctctactct
    11521 atcgaggcta ccatgaccat gaacttcgaa cgcgaggtcg aggaacgctt gctgcgctat
    11581 gtccaaatcg acacgacggc cgacgaatcc tcacccacct cgcccagcac cgagcggcag
    11641 tacgatctgc tgaacctgct cgttgatgaa ctgaatgaga tcggcgcgca ggacgtgcgt
    11701 ctgaccgact acggcgcggt gctggccacc atcccggcca caatcgagag tgacgcaccg
    11761 gtaatcgcgc ttttggccca cgtggacacc agcccggcct acagcggcgc gggcgtcaag
    11821 ccgctcgtcc accgcagcta tcagggcggc gacatccccc tgcccgacga cccggaacag
    11881 atactcacct atgagaactc cccctacctg aaggagcgca tcggtgacga catcgtgacc
    11941 gccagcggca cgacgctatt gggggcggac gacaaggcgg gcgtagccat cgtcatggcc
    12001 gccgcccgtc acttgctgaa caaccccgac atcccccacg gcacaatccg catcggcttc
    12061 acacccgacg aggagatcgg ccgcggtgtc cacgccgact tgcccgccga cctgggggca
    12121 accttcgcct atacgctcga cggcgcgcat cgcggcgaac tggtctatga gacgttctcg
    12181 gccgaccgcg ccgtcgtcca tgtccagggc gtgcccatcc atcccggctg ggccaaggac
    12241 aagctggtca acgccctgca tctggcggcc aagattgtcg acaccatgcc ccaggtcacg
    12301 ctgacgccgg aaaccaccga agggcgccag gggttcatcc atctctacgc catgagcggc
    12361 acggcggcgg cggccgaact ccatttcatc atgcgcgatt tcgagatgga cgagctggag
    12421 cgacagggaa aactaatcaa acaggtctgc gccacgatcc aggcgaccga gccccgcgcc
    12481 cacatcacct gcgaggtcct gccgcaatat cgcaacatgc gttactggct cgaaaacgac
    12541 atgcgccccg tggagatagc cgtcgaggcc taccgccggg cgaacatcga gcatttcttc
    12601 ctgcccatcc gtggcggcac cgacggctcg cgcctgaccg aaaagggtgt gccgaccccc
    12661 aacctgttca ccggcatgca aaatatccat ggcccactgg aatgggtcag cgtacaggac
    12721 atggcccgcg cgacggagat gtgcatcaag ctggccgagt gctggagcga ataacaaata
    12781 acacaccaag acagtcgggc ggcgtaaact tcagccgccc gacttcttat ttttccacaa
    12841 caggatgaat atccatgagc gaaaaaacgg ttcacaagcc ctctgacgcg acaccctatg
    12901 taacctggct ctacatgggc attatcgtcc tggcccaggc gcagatggcc ttcaacgtta
    12961 acgccatacc cgtctcaatc gggccgattg tcgaggagtt gaacatcccg gctaccggcg
    13021 tgggcacggc gctcgtcgtc tactctctct tcgtggccgc gttcgtcatg ctgggagcca
    13081 agatcggcaa aatctttggc gaacgactgg tgttccaggt gaccgcactt ctccatggct
    13141 tgtcgatggc agtcatggca gtcagccagg acgcaaccac catgaatatt gcccaggccc
    13201 tggccggtat cgccgccgcg gcgcttgtgc cgacactggt tgtgctgatt gccgccaact
    13261 ataacggggc gcaaaaggcg caggcgctgg gcatactggc ggccacgccc gccctttccg
    13321 gcgctctggc tttcttcgtc gccggcttcc tgggcaccta cctcagttgg cgctattcct
    13381 tcggcatgct gacctttatc tcgatcattg tcttcctgct cagcttccgg ttgaaaccca
    13441 tcccccggca gagcggagtc aagatcgact tcatcggcgt gagcctgtcg gccattgccg
    13501 tcattctcat cagctttggc ttcaacaatc tcaattcgtg gggtattgtt ttggcatcga
    13561 ccaacgcccc tttcagcctg gtggggcttt cgccggccct gctattcctg gtcttcggcc
    13621 tgatgctgtt ccaggctttc tttgcctggt cccaccaacg cacggaactg ggccgcgagc
    13681 cactcctctc gttggaggtt atcgactccc ccaaggaacg ggcgacgatc gtttccctgc
    13741 tcatcatcgg cgcacttggc ccggcggtca acttcctgat tccgctcttc atccagatcg
    13801 tacagggccg aacgacgatg caaaccgcgg ttgccgtcgt cccctacaca ttagctattg
    13861 ccacttcggc aatattcatc gttcgcttct atgatcgatt tacgccgcgc cgcattgccg
    13921 ttgtcgcctt tacgttagtt gccgccggcc tggccttgct gtcattcgtc gtcggcaaca
    13981 attggggcac agcgatggtc atctttggac tggtcatggt cggcctcggg gaaggttcgt
    14041 tgctgacact gctcttcaat gtgatggtca cctcggcgcc caagtcattg gccggtgacg
    14101 tcggcgcgct gcgtggcgtg gccaataatt tatcgactgc gttaggcact gcgtttgccg
    14161 gggtggtcgc cgtcagcctg ctaagcatgt tcgtgatggg tggcatcgat cagtcatcgc
    14221 ttcctcactc tctggttcgg caggtcaacc tggataacgt caactttgtt tccaatggcc
    14281 atctggatga agtaatggcc caaacgacag ccacacccga gcaagtcacc caggccgtgc
    14341 aaatcaatga aacggcccgg ctgagggcgc tcagggcgtc gttcctgatc ctgagtgcgc
    14401 tggcactgct ggcgatcttc ccggccatgg ggttgccgga ttattcattc ggtgacgaac
    14461 cgccggagga accggcggca aaacccaaac ggcgatcccg gaagaaaccc gcataggatc
    14521 taccgtcctg catatagaca gaaaatcaaa cgggagcctt ccaaccggct cccgtttgct
    14581 attgcgactc aggcgcggag gcaaagacat tcatgccaag atagtccgac aggaatctcg
    14641 ccaccaattg gccctttacg ctgttgccgg ccgaatcaag cggcggggcg aaaactccca
    14701 taccgccctt gcccggcgag acagtgataa ttccgccgct gacgccgctc ttgcccggca
    14761 gtccgacatc gaattcccaa tcgccggtgg tctcgtacat gccggcggtc gtcatcaccg
    14821 ccaacacgcg gcgacaatga gccgcatcga gcacctgctc gcccgtcacc ggattgatgc
    14881 cgccattggc cagtgtggtc gccatgatcg ccaggtctct ggcagtgaca tttagcgagc
    14941 attgccgggt atagatatcc gctgcctcaa ccgggtcgaa gtagagccgg ccatagtcgt
    15001 acatgatgcg agcaatcgcg cgattgcgat gattcgtggc cgaggccgac gcgtagactt
    15061 catcgtttag attgagcgtt cgcccggcaa atcgggacaa tccttcgtgg atgaagcgcc
    15121 atttggcttc ggccgtctct cctggaacca gcgtgacggc ggcgatagct cctgaattga
    15181 ccatcgggtt cgtcaaccgg ctggggttca gttcgatagc catgattgaa ttgaagggca
    15241 gccctgtagc attgacgccc agtttatccc gtgcttcctt cgcaccgata gcctcgcaca
    15301 ccaggccgaa cgtgaaaggc ttggacacgc tcatgatggt gaactcatag tccgcgtccc
    15361 cggcggccat cacctcacca tgggtgttga tcacacatac gccaaacaga tcgcgcggca
    15421 cacgagccag agcgggatag tgggatgcgt tttggccctc atcgttatcc ttgtagcgcg
    15481 cgtaagcttc gtccacgatc gtctgaacag cttcggtggg cggtaaatga ccggtcgata
    15541 cgtatttttt ccttttcttt tttgcttgcg ggttaacagt ttccttcatt ttttcggccc
    15601 cccccctcga aattatcctc ttgcaagtca cgacccaatg gcctgattat attcaacctt
    15661 gccgggaaat actgtcattt cgcgaaaaat gatgactcat ccgtttttgg cctctgtacc
    15721 caggaacaac caagttggta taattcggta gcctgtgtcc gggatcggcg cattgcaacc
    15781 catcagtcca atcgtcatga ataattttct tattgccttc acttttgccg ccatgtccgc
    15841 ctttcttgtc cgctcaagag aggaatggcc caacgtgtct tattgtgtca gcagtcaggc
    15901 gcatcggttc gcagccaggc taaccctcac agtgaactga tcatgggtgc cagacgagtt
    15961 aaccgtacac ggctccgccc caagcctgac aagccaaaag aaggcttcat ggctcatctg
    16021 ctcgacccca tcgaccggct ggccgagacc atctacagca tcctggtgct cctgaccttc
    16081 acactggcct tcaggctgtt ccatttgtcg cccggcgaaa ttgtttcgtc cgagtacgtc
    16141 aatgagttac tgatcggcgc gtcgttggcc attcttgcct ggggcattat cgacggcatc
    16201 gtctacgtgc tgaccgaggt gttcgagcgc ggcgagaaac accgcatact tctatacatt
    16261 gagtcggccg attccccggc cgaagccgta gaagccatcg cggacgaact tgatttcatt
    16321 ctggaaccca tcaccagcga aagccaacgc cggcacctct acgccgacat tctggagcat
    16381 ctccacgaca gcaccccgca accggttcac ctgaagcgcg aagacctgac cggcgggctg
    16441 gcttcggtgt tggttgccgt cctggcggtc ctgccgtcgc tcgccccgtt catcctgttt
    16501 cgcaacgacc acaacctggc gatccgcgcc tccaacgtcg tttccttcgc cgtcttgttc
    16561 tattccggct acgaatgggg caaatacaca ggcagcaacc cgtggaaaac agggctgttg
    16621 ctgctgacgg taggcgtgtt gctggtagcg attgcgattc cgtttggggg gtagccaccc
    16681 gtgcgtgata cgacgggcgt ttcgctcagc gaacgggata gggcacaact tcgatcaaga
    16741 gagcattttc cagtataagt tcggccatac tacccgattg cgcagcaacc acccaaatct
    16801 catcctgatt tccccgatcg aggccgatga tcaaatggct gtcgtcggca ctccagcgca
    16861 gcaatcccac ggaattcact ttaggtatag tcccgatgac ctgattttca ccccgctcca
    16921 cgtcgtagac aactacctcc acgtccgtgc cggacgctcc ccggcccaat gaataagcga
    16981 atcgccgccc atcgcgcgac caggcccagg ttacagggta ttcaatgtcg gcaatatgag
    17041 cgatcttttc accggcaatg gtcataattt gtaattcctc acgactattt acgagcaaat
    17101 ccgaattatt cggcagccag cttacaccac ccggataatc gggcgaaagc aaattacctt
    17161 ccagatcgaa caccttgttg cgcaccaaca ggtaatgtcc atcgggcgac caggatatat
    17221 cggaagtggt gtactcgata gcgaaggtct cttgaacggt tagtgtgcgc gcctgatcgc
    17281 cattggtcgg gactatccag taggcggcag catacttcgg attctccgga tctatcaatt
    17341 caggattgac tgaagtgagc acagcgatcc aacggccatc gggcgaccag tacgcatata
    17401 gctcccagag accctcatct tcgaagagcg attttggctc ggatcctgta gcgatatcga
    17461 gcgtgtagag cgtgccttct gatacatagg tgatcgcatc accggcaggg ttccacgttg
    17521 cgatcacccc atctggcagt agcttgcgcg cctcccccac ttccagatca agcagccacg
    17581 tgccggcgat ttccgggtcg ttattggaat tatggctcag gcctggaacc agcatatacc
    17641 gaccatccgg cgaaggcctt agccggaagt tgaggaagaa cataccgatc ggctcgatca
    17701 tctctcgctc gccatagagc ctttcgaaga tgaccggatc gacgaatggt aacggcggcc
    17761 acgtcttgaa ggcatagtag tcatcgagtg tcgagacgcc cggcggccga ttgggtgagg
    17821 tacgcatgtc cacaagggtc gtataaaaga taagcgtatc atccagaccg actatttccg
    17881 gtgtaataac cgtcgggctt ggaataggcg tgttcgtcgc ccgcacggcg gggactgtga
    17941 tcgtgggtat cgggatgggg gaggtggcaa ggtcagaggt tataacggcc ggctccaccg
    18001 aacccgtcgc agaaggcgag acagtttcag gtggcggggt aattatcgtc tcatttatga
    18061 tagtcgctac gcttggaact cccggaactt cattagtaca tgaaagcagg aaacttaccc
    18121 caccgactac tgttaacaca gctttgagca tcgtggataa atatttttgc atatgttccc
    18181 cacggatata aaagatggaa ggaatcaaga tcccttccat ccgacttgtt aagccaatgt
    18241 ggcgttaacg tcccatatta gcgcaagatt catcctgatc tatcgggaag tcattatctg
    18301 tgggccagtt ggacttcgga tagaaacctg acataccgac caagttttct ccatcgctac
    18361 cgttctgtac atggaaatgc agatgtgcat cgacgccgcc tgaatttcct gacagcgcaa
    18421 tctgactagg agaagacaca tgcgcacccg gttgcacata aatactctgt aaatgcgcat
    18481 aaaaagcttc ttttccacca gacgctttta tctttataag attgccatag ctactgttat
    18541 gctgtccggc ccaagtgaca acaccaactc tggcggagta gaccttattc cctacgccaa
    18601 cgccaatatc cgctgcccac ctgataatac cggtatggaa atccgaacca cattgcacgt
    18661 tccaaaactt gccatccgat ttcttaagca cgggcgatag ccaggaactc gtaagatttg
    18721 taacttcaat atcatcagat gtcagagcct cgtcagtaac ttcaggggtt cttgaattat
    18781 cgttgagatc aagcgattta aagtcggaac catatacctc acttagagta gacggcatct
    18841 ccttactgac acgaaaagta cgcaccattt ttctgtatac tttagaatca gcactttgtg
    18901 ggccgcttgg gatatttgtc caaatgaacc aaactattct gcctcgagct aaattggtaa
    18961 attgataagt tgtaacaggg gaaatacctt cctcatgtat agccgtaacg tcgccaacct
    19021 cgaagactct tctttgttgt ctttggactg aaccttcatc agaggcaata ctcaacgact
    19081 cgtagcgttc agtccactca ctcaacgact gactaggttc tatttcagcc aagtaaaggc
    19141 caatagttat ctgtgggcct gaaccttggt ggttttgaga accggtaaac tgcgacactt
    19201 ccacgggcac aaatgtgagc aatccactca aagctcttgg atcactatcg tcccgtggaa
    19261 tcacctgcca atccgacgga tacttgaccg aaaaatgaaa gcgtggatct acgtaagtta
    19321 cccatttaac atcatctttc ggcgcgctgt tttgcgcctg ggcaataacg acaacagcga
    19381 caatcagcaa catggctaca atagtagcag agattgaacg gtgcggaaat cggggcctca
    19441 tggtatcctc ctgaaagccg ggatgtttcg actgatttga acgcttggat catatcattg
    19501 cagtgaaggg gggggggcca gaggacttta ttcctattct ttgggaatca tttgcaaccc
    19561 tgaccgagag ttgacgtgac tcggctcttg aaggacaatt gcttcacttc aaggcttctg
    19621 cccccgtagc tcagaggata gagcgctggc ctccgaagcc aggtgcacag gttcgagtcc
    19681 tgtcgggggt acttccctta atgcatgaca catcgcgtta tgccttttgc ctccaaatcc
    19741 tgttacattt gtcactaagc acaaatagcc ccctccttta gaatgtagtc caggaacgta
    19801 aaacaacccg atattcgcgt tttgagcgcc ggcggccaat ctcactatcc gccgcgtggg
    19861 tttgtcttgc gttgcccgga ccctttccgt aggagaaaac gatgaccggt gcaggtttgg
    19921 acagcgaaac ccgcgatatg attctggata cgctgcgaaa gtacgccgag cgtaaactga
    19981 cgaccgacta tttactgcag ctcgaccatg atgaccgctt ccccgaggaa gttctcaaag
    20041 agttgtatga cccgatgcaa cttggattgc atctcctgtt tatccctgaa gagttcaacg
    20101 gcctcggcgg cggcgcgtat gatatctatc gcgtatccga attgatggcc gcaattgatt
    20161 tgggcgttgc cacgggtgtc ctggcgacgt tcctcggctc cgaccccatt cgcgtcggcg
    20221 gcacaccgga gcagaaagcg cactggatgg gccgcatcgc cgacgaggca ctgctgatgg
    20281 cctatggcgc gaccgagcca caggcaggca gcgacctggc cgccatgagc accaaggccg
    20341 tacccattca ggaaaacggc agcgtcaccg gctacaagat cagcgggcgc aagcaatgga
    20401 tcagcaacgg cggtgtggcc gacctgtaca cgatcctcgc cctggctccc ggcgggccat
    20461 catggttcgt cgtcgaacgt ggcgcggaag gtctcagcca tggcaagggc gaagacaagc
    20521 acggtatccg cgccagcaac acggccgccc tcttccttga agatgtccac gtccccaccg
    20581 agcgactgat cggcggcgtc gagggtcaag gtttggccca ggcccaggcc gtcttcggct
    20641 acacccgcct gatggtcggc gcgtttggcc ttggcgcggg ctgggaagca ttacgccgcg
    20701 ccatccacta cgcccacgag cgcatccagg gcggcgcgcc gctcagcgaa aagcagggct
    20761 acacccacaa gctcatcgtc cccaacgcct cccgtcttga agctgcccga acctacattg
    20821 agtgggtcgc cgaacgactg gacagcggcg aggaaggatt acagacagag ggggccgtgg
    20881 ctaaatacat ggcaaccgaa gcgggcaaca aagcggccga ggatgccatc caggccctcg
    20941 gcggctacgg ctacaccaaa gagtatatgg tcgagaagat caagcgcgat gtgcgtatca
    21001 cctgcatcta cgaggggacc tcggagatca tggaatggac catcgcccgc gaccgctggc
    21061 agcaacatct gaagacgcag ggagcgttct acaacgactg ggcagcgcga ctggacaagc
    21121 tccacgccgc cgagccggac aacggcgcca acttcgccgc gctggcgatg cgcgctctgg
    21181 ccatcatcct ggaacgcgcc cgaattgatc gcctcacccg caatcagcac gtgctgttcc
    21241 gccttggcga gctaatcgcc tgggccgaaa cggcggccgt ctttgccgaa cgtgtcacct
    21301 cccatccaac agaggccatc catctcgacg tgccgactcg acaggcactg gctcgcatcc
    21361 acggccgcaa cgccgcgctc aaggtcgcca ccgacggcct gcaatgggca atcggcgcgg
    21421 gtcagaccga cccgaatctg gccggctcgc tcaacctgcc cggcgtctac gctgcgcagg
    21481 ccggccagat cgacgatatg aatttcgcgc gcgaccaact caaccgcgca ttcagttaac
    21541 agaaatatca cagctattac agaggtcacg gattcacagc cttgcctgcg taatccgtga
    21601 cctctgcgga tgatcttcat cctaaaggga aaacaatcat ggattcagca gatcgcgcaa
    21661 ttgctatcgt cggggtggga gccatccttc ccgatgcgcc gagcgcggcc gcgttctgga
    21721 acaacatcgt cggcaagcgc tatagcatca ccgagacgcc acccgagcgg tggagcatca
    21781 ccgactacta cgactccgac cccaaaacac ccgacaaaac ctacagcaag atcggtggct
    21841 gggtgcgagg atttgagttc gactggaaac gattccgcat gccgcccaaa gtagccgcat
    21901 caatggacga aggccagcag tgggccgtca ccatcgccgc cgatgcactg gccgactatg
    21961 gctatcccga ccggccgctc gataccgaac gaaccggcgt cattctgggc acggccatgg
    22021 gcggagagct gcactatgtg acatcacagc gcatcgtctt ccccgaatat gtacgctggc
    22081 tgcgcagcac gcccgagttc gccaaactct ccccggcgac ccaggaagcg ctcatcgaac
    22141 agttccacgc cgcactcgac caagatgtac cgcccatcac cgaagactcc atgcccggcg
    22201 aactgcccaa catcgtctcc gggcgtgtgg ccaacatgct caatctgcgc ggcccgaact
    22261 ttatcaccga cgccgcctgc gccagcagct tcgccgccgt cgaggcggcc atccagatgc
    22321 tgaacgagaa caaggtggac gccgtgctca ccgggggcgt cgaccgcaac atgggcgcga
    22381 cgaccttcgt caaattctgc aagatcggcg cgctgagcgc caccggcacg cggccgtttg
    22441 gggatggcgc cgacggcttc gtcatgggcg agggcgcggg cgcgttcctg cttaaacgat
    22501 tgtccgacgc cgagcgcgcc ggcgacaaaa tctacgccgt cattcgcggc gtggccggca
    22561 gcagcgacgg caagggcaag ggcattaccg cgccgaatcc tgtcggccaa cagctcgctg
    22621 tgcaacgcgc ctgggaatcc gccggactag acccggccac cgcatcactc gttgaagccc
    22681 acggcaccag cacccgcgtc ggcgacgtgg ttgaggtcga aagcctgtct caaatgttca
    22741 gcggtgcggc tcgcggcagc atcggccttg gctcggccaa gagcaatatc ggccatctga
    22801 aagcgggcgc aggagcagcc ggcctgttaa aagccaccat ggcgctccat cacaagatat
    22861 tgccgcccac tctccacgcc aacccgacca atcccaacat cgactttacg gccaccccct
    22921 tctacccgct ccatgacgca cgggaatgga caaaaagtaa cgggactcct cgccgcgccg
    22981 gcgtcagcgc ctatggtttc ggcgggacga acttccatct ggtacttgag gagcatatgc
    23041 cgggagcgct tacacagcgg ccgaagctct atgcgggagc aggggagaaa gggagcaggg
    23101 gagcagggga aaaagaggcg agttccgaac ttgccactcg tcagccggcg attgttcctc
    23161 cgcgaggcat cgtggcatta ggagccaatt cgcccgacga gttgaagaac aagctcgacg
    23221 aactgctcct ccgcgtcgat gcgggctgga cgccgcccaa cgcgctgccc cgaccggtcg
    23281 acatccgcgc caaagagcga ttggtcatcg acttcggcga tcacgaagag ctgctcgatc
    23341 gcctgcaaaa agcgcgcaag gtgatgggct tcgacacacc ccaggcctgg aaagcgatgg
    23401 aagcccaggg catcttccgc ggccgaggcc ccaaaccggg caagctggct ttcctcttcc
    23461 ctggtcaggg cagccaatac gtcaatatgg gtcgcgagct ggcggccgtc tcccccacgg
    23521 tgcaggccac actcgatgag gccgacgcca tcatgcagcc catcctgggc aagccgctga
    23581 cctcctatct gtttgtcgat gccaacgaca aggccgccgt cagtcaggcc aacttcgacc
    23641 tgatgcagac ggagatcacc caaccatcca tgctgactct cgacgaggct atccgccgcc
    23701 tgctggccga ctatggcttt gagccggata tggtcatggg ccactcgctg ggcgagtatg
    23761 gcgcgctggt ggcagcgggc gtcctgccct tcgccgaggc gctggaagcg gccgccgggc
    23821 gcgggcgcga aatggccaag gtcaaggtgg acgacaacgg ctggatggcc gccgtactgg
    23881 cccccttcga gatcgtcgag gcgacgctca aggagatcga cggctatgtc gttccggcca
    23941 acatcaacag caccagtcag gccgtcatcg gcggcgaaag caaagccgtc gaagcggcca
    24001 tcaagctctt ccaggagcgc ggctaccagg ccatgcaatt gccggtcagt cacgccttcc
    24061 acacccgcat cgtcgccccg gccagcgcgc cgctgcgcaa gatgctcgac cggatgcaca
    24121 tcaccccgcc acggctgccg ttggtcgcca acgttaccgg cgatttctac ccggcaagcg
    24181 agaacgagat caaggatttg ctggaaaaac agatcgcttc cccagtgcaa tgggtgaagg
    24241 gcctccagac gatgcacaat gccggtgttc gcacctttat tgagatcggc ccgaagcgcg
    24301 cgctacgcgg ctttgtaaag gatgtcttcg gcgaccgcga cgatgttgta gctctcatga
    24361 ccaaccaccc caagggtggc gagctaccca cattcaatca agcattgtgc gggctgtatg
    24421 ccgcgggata cggtgctgaa gagagcgccg gtcaacgaca accggccata gcagcgaccg
    24481 cagatgacgg acgactgacg actgacgtga atagaacggc ggccaatgga acgattgccg
    24541 tcgccaacga gaatgatatg aataataatg cacaaccgac ggccgcgtct ctggacacgc
    24601 tggctcaggc gttggctcag gtgctgcaaa gcaacggcca tgccgcctcg cagcgcgtct
    24661 acgatcgaaa cgagacaccc ctcggctcaa tcgtcatcag cggcaccggc ctgggcctgc
    24721 ccggcataga gaagccggtg atggaccccg acaacgccat gcgcatcctg cgcggcgagc
    24781 aattcgtaga tttgatcccg gagcggttcc gcaacctcat cgtcaggaag cagatcaccc
    24841 gtcttgtgaa aggtgaggac ggcagcggcc gcttcgaggc gatctccgac ccgaatgaag
    24901 tcatcaagtt ggccggtcgg cccggctcct tcgatctggc cgaggaatac ggcgtgcccg
    24961 ccgaattgat cgaggcgctg gatacaacca cgcagctggc aatggcggcc ggaatcgacg
    25021 cgctacgtga ggcaggcatc ccgttggtgc agacctataa gcgcaccagc accggcaagc
    25081 atctgcccga acgctggatg ctgcccgagg ccttgcgcga cgacaccggc gtaatcttcg
    25141 ccagcgcctt ccccggcggc gaccgcttcg ccgacgagtt caagcggtat tacatcaacg
    25201 tgaaccgcac ccagcaactg gaaatgctgg aagatttgcg caactacgcc actgatccgg
    25261 caacacagag cgaggtcaac cgacgcatcg acgagttgcg cgaccagttg gagcgcgaac
    25321 cctacacctt cgatcgccgg ttcctgttcc gcatcctggc catgggtcac agccagttcg
    25381 cgcaatacat cggcgcgcgc ggccccaata cacaagtgaa cgccgcctgc gccagcactg
    25441 cccaggccat cgccatcgcc gaggactgga tccacagcgg ccggtgccgt cgtgtcgtcg
    25501 ttatcggcgc cgacaacgtg accggcgaca acctgatgga gtgggtcggc gcaggcttcc
    25561 tggccgtggg cgcggtgacg acgaaagatc aggtggctga agccgccctc ccgttcgaca
    25621 aacggcggca cggcacgatc atggggatgg gcgcgtgcgc gctggtcgtc gagagcgaag
    25681 acgccgtgcg cgagcgcggg atgcgcggca tcgtcgaact gctgagcagc gagacgaaca
    25741 acagcgcctt ccacggcacc cggctggacg tttcccacat tggcatggtc atgaacagcc
    25801 tgatcactgc tgccgagcgc cgctttggcc tgaaccgtta cgccatcgcg ccgcagaccg
    25861 tcttcatgtc ccatgagacc tatacgcccg cccgcggcgg cagcgcctcg gccgaagtca
    25921 tggctttgcg ccacaccttt ggcgaagcgg ccaacagcat catcatggcc aacacaaaag
    25981 gcttcaccgg ccacccgatg ggcgtcggca tcgaggatgt gatcgccgtc aagattctgg
    26041 aacacggcat cgtcccacct gtgcccaact tccgcgaggt cgatcccgat ctggggccgc
    26101 tcaatttgag ccgtggcggc cgctatccgg ttcaatacgc cattcatctg gcggccggct
    26161 tcggttcgca aatctcgctc accttcacgc gacgtatccc cggcagcctc gatcgcgtcg
    26221 ataacccgcc catgtatcgg cgttggttgg ccgacgtcag cggctacgac acagccgaaa
    26281 ccgaagtcgt caagcgcacc ctgcgcgttg tcgcccaagg ggcgcccacc cgtccgccga
    26341 tgcccaatcg ctggcagccg ggcaccggcc cgacgatgcg cgccgcggcc ggcggcgaaa
    26401 tagcggcagt ggaactgcct gcgcgaatgg ttaccccaac cgtcgatcgc cggccgtcga
    26461 ccgctgctcc cgtccagcaa cccgtagata acgggcaaaa gcccgtagaa gtcaagccgc
    26521 aggtcgtaat cgaacagccc cggcccgagc cggtcgtggt ggctgccata ccgaccgcag
    26581 acagccggcc gccgaccacc gtcagtgaag cgcctccggc agcggtcgtc gatcagccgt
    26641 cggccgtcga tccagtcacc gacaaagtcc tggccatcgt cgccgagcag accggctatc
    26701 cgcaggagat gctcgacctt gatctggata tggaagccga cctcggcatt gacacggtta
    26761 agcaagccga aacgttcctt gccatccggc aagcgtttga catcccccgc cgcgacaatc
    26821 tgaagttgcg cgactatccg acgctggacg ccgtcatcgg cttcgtgcgt cagagccggc
    26881 ccgatctggc cgcagccgcc gtaaaagagg agccgcaacc tgcccctgtg acccatgccc
    26941 caacagtgcc cgtccttgca acaccggcca ccgacccagt cactgacaaa gtcctggcca
    27001 tcgtcgccga gcagaccggt tacccgcaag agatgctgga ccttgatctg gatatggaag
    27061 ccgatctcgg cattgacacg gttaagcaag ccgaaacgtt cctcgccatc cggcaagcgt
    27121 ttgacatccc ccgccgcgac gatctgaagt tgcgcgacta tccgacgctg gacgccgtca
    27181 tcggcttcgt gcgtcagagc cggcccgatc tggccgcaac cgccgcaaaa gaggagccgc
    27241 aacctgctcc tgtgacccat gccccaccag tgcccgtcct tgcaacaccg gccatcgacc
    27301 cagtcaccga caaggtcctg gccatcgtcg ccgagcagac cggctatccg caggagatgc
    27361 tggagcttga cctcgatatg gaagccgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27481 nnnnnnnncc ggctatccgc aggagatgct ggagcttgac ctcgatatgg aagccgacct
    27541 cggtatcgac acggtcaagc aggcggagac attcctcgcc atccgtcagg cgttcgacat
    27601 cccccgccgc gacgatctga agctgcgcga ctacccgacg ttgggcgcgg tcatcggctt
    27661 cgtgcatcag agccgacccg atctggctga gccgaccgtc gaccatcgac cgccgaccgc
    27721 tgtcagtgaa gcgtctccgg cagcggtcgt cggtcagtcg tcggccgtca cccctggcta
    27781 caccctggcc gacgccgatg caatgccccg ccgcgtgccg gtgccatcct tgcggccgtc
    27841 gctcgacctg tgcaagccaa ccggcgtcac actggatgca aacagccgga tcgttatcat
    27901 gatggatcgt gggggtgtgg gcaaggcact ggtaacccgc ctggagaagc tcggcgcgac
    27961 gccgctaatc attaacgata tacccacggc tgaagcactg gaagctcaac taaagacatg
    28021 gctggccgaa ggctccattc agggcgtcta ctggctgccc gcgctcgatg tcgagccgga
    28081 gccggatgag atgacgctcg aagaatggcg cgaacacaac cgcgcccgcg tcaagaatct
    28141 ctacaccaca atgcgcacgc tctatgacgc ggtgaaggat cgcggcacgt tcctcgtctc
    28201 gggtacgcgg ctcggtggtc tccacggcta tggaccggag ggagccacgg ctccgctggg
    28261 cggcgcagtg agcggcttca ccaaggctta caagcgcgaa cgcggcgatg ctctggtcaa
    28321 ggtcgttgac ttcgaaccgg gacgcaagac cgcgccaccg gccgacgcgc tgctggccga
    28381 gacgctgctc gaccccggcg tcgtcgaggt tggctactat caggacaacc gatatacggt
    28441 gactctggtg gaggaaccgg cggccaacgg gcaacccggc ctggcgctcg gcccggacag
    28501 cgtctacctc gtcaccggcg cggctggcgg catcaccagc gccatcatcg gcgatctggc
    28561 ggccgcgggc ggtggtggct cattctattt gctcgacctc gtgcccgaac cggcgcgcga
    28621 cgaccaacag atagccttgt tccggcaagg gaaggacgcg ctcaagcaac acctgatcgc
    28681 cacggccaag actgccggcg agaaggtgac cccggcccaa atcgacaagc agttgatggt
    28741 tgtggagcga aatgaagcag ccttgcgcgc catcgaatcg gtggaagcgg cgggcggcaa
    28801 ggcccattac cgcagcgtga atctgctcga tggcctggcc ctgacggccg tcgtcgacga
    28861 aattcgtcac caacatggcc ggatcgacgt gttggttcat gccggcggca ttgagatcag
    28921 ccgttcgctg gccgacaagg attacaccca gttcagcctg gtctacgaca tcaaggccga
    28981 cggtttcttc agcctcatgc gcgcggcgaa agacatgccc atcggtgcga cggtggcgtt
    29041 tagctcggtg gccgggcgct tcggcaataa cggccaaacc gactatagcg ccgccaatga
    29101 cctgctctgc aagttgacga gcagtctgcg ccgccggcgg cccactacga agggcatcgc
    29161 catcgactgg acggcatggg gcgaaatcgg catggcgacg cgcggctcca tccccaggat
    29221 tatggagatg gccggcatcg atatgctccc tccatcggca ggtatcccca cggtgcgccg
    29281 cgaacttgtt gccggctcct gtcggggcga gatcgtcgtc ggcggaaggc tgggcatcct
    29341 ggtcgatgag tgggacgaga ccggcggcct ggatacggcg aaggccaacg aatggctggc
    29401 tgcccaacac ccgcccttgc atatgctcgg tgaagtaaaa gctgcaaagc tctatggcgg
    29461 actggaggtc gagacgacgc tcgatcccaa cgagcagcca ttcctgtacg accacgccat
    29521 ggacgggacg ccacttctgc ccggcgtcat gggcactgaa gcgttcggtc aactggcgct
    29581 ggcgctggct cccggctatc gggtggccgc cgtatacgat gaggagttcc acgcgccctt
    29641 caagttctat cgcatggaga gccagacgct gcacttgagc gccatcgcca cacctgcggg
    29701 agataataat ggcttgatcg ttcaggcgag cctgcgctcg ctgcgcgacc tgggcaagcc
    29761 tggactgccg ccacaagaga agctccactt caccggcaag gtgaggctgg tacaagaact
    29821 gggccaaccc gtgatcgatt ttactccgcc ggcatccgaa tccctgtcga tttcggccga
    29881 ggatgtctat cggatctact tccacggccc agcctatcag gtattggaac gggttgacgt
    29941 tcacgacgat cgagctatcg gcttgctggc ggcggcgttg ccctccaaca cgaatccggc
    30001 cgatgccgcc tcaatcatgg ctccccgact catcgaacta tgctttcaga cagccggcgt
    30061 atgggacatc aagactaacg gcacgatggc tctgccgctc ggcctgaact cagtcaccgc
    30121 ctatcggcaa ccgatggaaa ccaacggcaa tcggctctat gccgtggtga cggctctcga
    30181 tggtggcgaa gcgtatcatg cccaggtcgt ggacgactcc ggcgcagttt atgtcgactt
    30241 gaaggggtat cgcactgtcg cactccccgg cagcgttacg ttataacgtc gcaccttatc
    30301 aacaatatcg gggcgccaac cattggcgcc agtcataggg atcccgtcga gcgttaggcc
    30361 ataacgaccg actgcatctt catctctttt cttctcgaca cagattgccc gcgagcttca
    30421 aaccgcgggc aatctgcatt tatggcaggg acaggtataa tgaactggat gaacgcgaca
    30481 accaggagcg gcgtcatcac cactccccaa ggacctctcc actggcttgt ccttcacgcc
    30541 ggacaagaca acctgcttgc ctcggatact ccacctgact tcttacatcc aaccgaaatc
    30601 cggcattggc agacgctcaa gatcaacaaa cgccggcggg agtggacgct cggccgccgg
    30661 gctgccaaag agttgattat cgagcaaata tacgagaata atggccatcg cctcgatctg
    30721 gcccggatcg cgattgttcc ccatggtgac ggctggccca tggtcatgct gcccgatcag
    30781 gccgatttcc cggcgatcac tctttcgatc agccactcca gagacaaggc cttttgtgcc
    30841 acgaccgaag gcgcagaccg gctactggga gccgacatcg aggcaattga gccgcgcacg
    30901 cccgccttcg tggaggagta cttcacccca ctcgaaaacc ggttcctgac cgtggttcct
    30961 gcggatcaac gcatgaccct gataaacgcc gtctggagcg ggaaagaggc cgccctgaag
    31021 gcgattcgtc gtgggctggc cgaagacacg cgtattgtct cttgcctgcc ccaccccgcc
    31081 atgggcgata aagcccaatg gctgcccatg cgcatcgaat ggacggcaga acgcacgcaa
    31141 cagccaatgc cgccgttaag aggctattgg cggcatgaga acggcttcat tatgacactg
    31201 gcctttgctg ccgatgcagc gccgtaatgc aatatattta agcagtgacc gccgg
//
LOCUS       DAOMQG010000004        28937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_712_length_28937_cov_11.427048, whole genome shotgun sequence.
ACCESSION   DAOMQG010000004 DAOMQG010000000
VERSION     DAOMQG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 28937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28937
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_712_length_28937_cov_11.427048"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..819
                     /gene="map"
                     /locus_tag="PLC06_00510"
     CDS             55..819
                     /gene="map"
                     /locus_tag="PLC06_00510"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRO88957.1"
                     /translation="MRLKTPKEIVKLRAAGRLVAECFDILQENIKPGVSLKTLDKMVE
                     DHIYRLGAEPLYKGYRGSAREHPPFPGVICASVNHEICHGLPDGRVLEEGDVIGVDIG
                     LRLKGYCGDACVTYPVGKVAPHVERLLDIGREALRVGIEAAQYDNHLNDIGRAIEGYA
                     HTQGVTVVHEWGGHGIGRVLHENLSVSHIRQSSPGPRLVPGMTFTIEPMINEGTYEWI
                     LLPDGWTVITADGKLSVQFEHTIAITRNGPDILTRL"
     gene            complement(966..>1694)
                     /locus_tag="PLC06_00515"
     CDS             complement(966..>1694)
                     /locus_tag="PLC06_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRO88958.1"
                     /translation="QLRHHLEIQRMSKSKGNVVNPDELVAQFGADAVRAYLMFFAKWD
                     QGGPWNYGGIKGPQRLLQDIWEIGRTDYAPEHEDEAATRALRRKTHQTIRKVTQDLDE
                     FSFNTAVAAIMELRNTMSAALRAAEASRAAWDEAVDTILLLLAPISPHITEELWSQRG
                     RPYSIHQQSWPQWDEDAAREEMISLVIQINGKTRDKLDVPAGMDDDQLSRIALESEKV
                     ERLLDGKQPRKVIVVRGTLVNIVI"
     assembly_gap    1695..1794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1795..3599)
                     /gene="leuS"
                     /locus_tag="PLC06_00520"
     CDS             complement(<1795..3599)
                     /gene="leuS"
                     /locus_tag="PLC06_00520"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRO88959.1"
                     /translation="MSESYEPAALEPKWQEKWEADGLYRSVVDWNKPKHYALTMLPYP
                     SGDLHIGHWFAMTPSDARARFMRMRGYNVLFPMGFDAFGLPAENAAIQRNIHPKTWTY
                     ANIERMRKQLRSMGAMFDWEREAVSCDPAYYHWTEWFFSQFYKNGLAYRGEGMVNWSE
                     TLQTVLANEQVIDGKDERTGQPVVQKLMEQWFFAITRYCDELLNFERLDWPDPVKLMQ
                     TNWIGRSEGARVVFMSEQGDPIEIFTTRPDTLWGATFMVLSPEHSLVDKLTSDTQREA
                     VEAYREQAARATEIERLSDTREKTGVFTGGYAINPVNEERVPVWIADYVLSTYGTGAI
                     MAVPAHDQRDFDFARKYGLTIIPVIQPEGETLDGETMEAAYVGPGSMINSAQFDGTPV
                     TSAKGWANPSVSAVGDWLSERGIGSKSVNYRMRDWLISRQRYWGAPIPAIYGPDGIEM
                     VPDDELPVLLPDDVEFMPTGRSPLTYHAAFLHTTDAEGRPARRETDTMDTFMCSSWYQ
                     YRYLSPRYDQGPFDPAEAAYWLPVDVYTGGAEHATMHLLYTRYFTKAMRDLGMFAEVE
                     QMMRANDRDPRGAFDEPMTMLRNQGQILGAERKGDT"
     gene            complement(3709..3781)
                     /locus_tag="PLC06_00525"
     tRNA            complement(3709..3781)
                     /locus_tag="PLC06_00525"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3746..3748),aa:Ala,seq:ggc)
     gene            complement(3992..4064)
                     /locus_tag="PLC06_00530"
     tRNA            complement(3992..4064)
                     /locus_tag="PLC06_00530"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4029..4031),aa:Ala,seq:cgc)
     gene            4270..4476
                     /locus_tag="PLC06_00535"
                     /pseudo
     CDS             4270..4476
                     /locus_tag="PLC06_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(4492..>5265)
                     /locus_tag="PLC06_00540"
     CDS             complement(4492..>5265)
                     /locus_tag="PLC06_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRO88960.1"
                     /translation="QEPLNMKVLIAGEIPFVDEVERLCNRAAHDVRVFLVEDFLGAIE
                     AEFDMDHLADIEIAIELHNESAAAKQELLLALSSLLPDDALILTSSLATSATLAASWT
                     RRPERVVGFAVLPPVPDEGIIELTSALQTDESALVQAELFWRGLGQETIVVADGPGLV
                     RARTVCCLINEAAGLLMEGAASADDIDKAMRLGMNYPIGPLAWADMIGLDAVLGVMTG
                     LFEEWGDDRYRPTPLLRRMVAADMLGRKTGRGFFIYEEE"
     assembly_gap    5266..5365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5366..5895)
                     /locus_tag="PLC06_00545"
     CDS             complement(<5366..5895)
                     /locus_tag="PLC06_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO88961.1"
                     /translation="MPTLHTNGINLKYDEQGQGEPLLFLHGLGSQGEDWGFQVPFFAP
                     HYRVVTVDLRGHGESDKPAGPYSVSMMAGDVVGLLDALKIESAHIVGLSMGGMIAFQL
                     VVDKPERVRSMVIVNSGPALVARNFGEWLRIQQRLLLARLFGPSRTGAFLSKRLFPKP
                     EQEAIREQFVERWALN"
     gene            complement(5867..6886)
                     /gene="trmB"
                     /locus_tag="PLC06_00550"
     CDS             complement(5867..6886)
                     /gene="trmB"
                     /locus_tag="PLC06_00550"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRO88962.1"
                     /translation="MSFVDWYKVEQLSWPADWASLFGRNAPLVLEIGFGSGLFLVDLA
                     RRFPDANILGLEISIPSLRNAMRKVERLGLTNVCLLQSDAHSALQILCEPSTIAAAYI
                     NYPDPWPKKDHTDRRLIDGEFLALLASRMMPEAFLDIATDHDEYAEQITASLGETPYF
                     HSRTSQPFEREDEGRVRTKYEQVALDEGRSPRYYKWQRNTVPVVERFLIPKELSMPHV
                     VFRLPATVEEIGRQFRPRVVTVESTRIRFIETYQSFHDGKLLIETYIHEEPLMQRIGL
                     EIRPRASGEIVISMAEVGFPRPTQGVHRAIAALVDWLRDEYPSLVIVQTNLQGEHADA
                     PYKRN"
     gene            7017..7910
                     /gene="rocF"
                     /locus_tag="PLC06_00555"
     CDS             7017..7910
                     /gene="rocF"
                     /locus_tag="PLC06_00555"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRO88963.1"
                     /translation="MQRKIRILGVPMDLGQNRRGVDTGPSAVRYANLKTRLEELGHIV
                     HDEGNIAVPNPEEHVAEGTGRRLQTITTICQTVYDRACPWIEAGEYAIFLGGDHSISI
                     GSVAAAAAHHEPIGVIWIDAHSDFNTPETSPSGNIHGMPVATLIGEGPEPLVSIGHPG
                     PKVDSTQIVQIGIRNLDGIERERLIHSGIHVFTMRHVDELGMAAVARQALDRLHHLPR
                     IHISLDMDSLDPSEAPGVGTPVPGGLTYREAHLLMEILGDSGRVESLDIVEINPILDE
                     SNKTAELAVELAASLLGRRIL"
     gene            7926..9749
                     /locus_tag="PLC06_00560"
     CDS             7926..9749
                     /locus_tag="PLC06_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO88964.1"
                     /translation="MAALTRGLGAEKYDRIYSDHDLVVRIVNYFKPYKTKVIVIAIAL
                     FTIAAMGALLPILIADSLNSSTDGGAFPLHLVILVFVIGVATWVFSAIRRYLTAQVIA
                     DVVMTMRDDAFNAAMRQDLSFFDEFNTGRIVSRITSDTQDFGEVITIATDVINQLTVA
                     FILIFVLFTIDWRLTLAVLAMTPLVAVVALAFRHLARDVTRQSSRALAEVNKAIQEAV
                     SGIRVAKNFRQEGAIYNEFMQVNDQSYRINIRRAFVLSNIFPTLNALAGLGTAALVYF
                     GGRSAVTGAITIASWYLFVTTVDRFWFPVTNLSAFWSQFQAGLASTERVFGLIDADSS
                     VVQTADKPVHSLRGEIVFDHVSFRYSSQEQVLNDFSLRIQPGESIALVGHTGAGKSSI
                     IKLVARFYEFQEGTITVDGTDIREFDLKSYRDKLGIVSQVPFLFAGTVADNIRYACPS
                     ATDEQIMTVACKIGNGEWLETLPDGIYSEVGERGSRLSMGQRQLVALTRVLVQGPSIF
                     ILDEATASVDPFTESQIQQALDLIMANSTAIIIAHRLSTVRSADRIIVLQKGRIIEEG
                     SHSWLMKQGGHYAELYDTYFRHQSPQAIHTPSWMQERSQSA"
     gene            complement(9805..10434)
                     /locus_tag="PLC06_00565"
     CDS             complement(9805..10434)
                     /locus_tag="PLC06_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO88965.1"
                     /translation="MNQVLNIVLLSLVGLLVLAALYFASKALGARSSVHRQAYNVGQA
                     EAKRTSQIYWVRAAFSLIVGLIVLGIFAVRPLFSGLGATIMNPTPTVPAVEIEPTSAI
                     ATEPAVQATVEPTPTSPPSSPTPQVTAEPTATTAPVTAAVSSAVGVWLRGAPSTSGEQ
                     LEWLLDGTVVTLLAGQETADDLSWQQVRTEAGVEGWVARDFLSVAEPAP"
     gene            complement(10456..11946)
                     /gene="mazG"
                     /locus_tag="PLC06_00570"
     CDS             complement(10456..11946)
                     /gene="mazG"
                     /locus_tag="PLC06_00570"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRO88966.1"
                     /translation="MTITIVGLGPGDAGLITIQAWHLLSAADTVYLRTARHPAVDGLP
                     ARIKRHAFDEIYQTAERFDEVYRQIAEEVLRIGIEKDILYAVPGNPFVGETTVATILR
                     GAANAGIPVRIVPGISFVEPALAAMGIDALDGLQIFDGIEIASALYPPLNPDVPLLLG
                     QVYSRMVASELKLSLMGIYPAEHKAALIHSAGQEDQLVEWSPLYEIDRSDYIDHLTSL
                     YVPPLPLKAGLPALAETVAVLRSPGGCPWDQEQTPQSMRTGFLEEAYEVLDALDAEDV
                     DNLREELGDLLYHIVMQTQMATEAGEFTLTDVIAGIEAKLKRRHPHVWGDWQVSDSAE
                     VLRNWEIIKRMEKGDRAADEFVSRLSGIPSSLPTLMRAQKIQSRAAKTGFDWPDISGV
                     YEKLEEELDELREASTPEELRLELGDVLFVMTNLAQWLGVEAEIALREANARFMRRFQ
                     LVEKFIVERGLEWSNLSFVEMDALWEEAKAALANSDVAAKVDDESA"
     gene            complement(12010..12417)
                     /gene="rpsI"
                     /locus_tag="PLC06_00575"
     CDS             complement(12010..12417)
                     /gene="rpsI"
                     /locus_tag="PLC06_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRO88967.1"
                     /translation="MSEYFEGIGRRKEASARVRIYFREASQEEGPSFIVNEKPVQEFF
                     PRYGDYQTLIGPLEDSGLMGKVDVTVLVQGGGITGQTSAVRLGLARALVKYDENLRTP
                     LRAGDHLTRDARVKERKKPGLKRARKAPTYTKR"
     gene            complement(12453..12881)
                     /gene="rplM"
                     /locus_tag="PLC06_00580"
     CDS             complement(12453..12881)
                     /gene="rplM"
                     /locus_tag="PLC06_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRO88968.1"
                     /translation="MKTYVTKPADIERAWYVVDAEGQTLGRLASSVATILKGKHKPTY
                     SPAVDCGDFVVVVNADKIAVTGRRLEQKKYYRHSLYIGGLKEITLRDQLQQHPERVVE
                     SAVRGMLPKNTLGRKMFKKLKVYAGTEHPHQAQQPQPLEL"
     gene            complement(12921..13649)
                     /gene="truA"
                     /locus_tag="PLC06_00585"
     CDS             complement(12921..13649)
                     /gene="truA"
                     /locus_tag="PLC06_00585"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRO88969.1"
                     /translation="MQYDGTNYYGFQRQRRGVPSVQAELEHALARLVDHPVRVLGAGR
                     TDTGVHALGQVIGFTIEWPEKHGSEALHRALNANLPQDIVISELAMAQPRFHPRFDAR
                     RRSYVYHILNVPIRQPLWRHRAWHVAQRLDPERMNEAAALLIGVHDFATFGRPTVGES
                     TIREVYSAVWRRDAQMVTFEISANGFLYRMVRSIVGSLKAVGCGKWSVGDFADALAAR
                     ERKRSATAAPAHGLYLVSVEYDNE"
     gene            complement(13716..14141)
                     /gene="rplQ"
                     /locus_tag="PLC06_00590"
     CDS             complement(13716..14141)
                     /gene="rplQ"
                     /locus_tag="PLC06_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRO88970.1"
                     /translation="MRHKVFGRRLNRDTAHRNALRRNLIADLLVFEQITTTEAKARTI
                     RPAAEKMITLAKRGLAQSDEQPGSAIHARRLAASRLPNTRTTEAEDGTFEDVDVVRKL
                     FEEIAPRYANRPGGYTRMVKIGRRPGDNAEMAVLMLVED"
     gene            complement(14171..15115)
                     /locus_tag="PLC06_00595"
     CDS             complement(14171..15115)
                     /locus_tag="PLC06_00595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRO88971.1"
                     /translation="MPKIESTAMTAEYGRFVIGPLERGYGTTVGNALRRVLLASLPGA
                     AITSIRVTDVPHEFSAIPGVREDMMQFILHVKQLRLRLVNTDIARMRLEVQGAGPVTA
                     ADIQVPPEVEIINPDLYLFTVDADDTFLEIELMAETGRGYSPAEDRGRLPIGELPVDS
                     IFSPIRRVAYEVEKTRVGQAADYDRTVMEIWTDGAIKPEEALAQAAQILIQHLRPIAG
                     VSEETFVTVEEEREEETIPNEIYDTPIEQLDLSVRVFNSLKRTGITKVGEMLEMLDRG
                     EETMLAIRNFGDKSLDELKQQLRLKGFLPEEDEAKRSA"
     gene            complement(15249..15887)
                     /gene="rpsD"
                     /locus_tag="PLC06_00600"
     CDS             complement(15249..15887)
                     /gene="rpsD"
                     /locus_tag="PLC06_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRO88972.1"
                     /translation="MARYSGPVCRLCRREGEKLFLKGSRCLTPKCSVERRGYPPGQHG
                     RENQFRRGRASDYLLQLREKQKARRIYGVLERQFSRYFERASRQAGLTGYNLLAILER
                     RLDNVVYRLGLADSRAQARQLVQHGHIMLNGRKTNIPSALVAPGDVVSVRKESTRRTY
                     FKDLRQNMDDRRVPRWLTVDTANLSGRVMHAPAREDIDISLNEQLIVEYYSR"
     gene            complement(16012..16407)
                     /gene="rpsK"
                     /locus_tag="PLC06_00605"
     CDS             complement(16012..16407)
                     /gene="rpsK"
                     /locus_tag="PLC06_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRO88973.1"
                     /translation="MGRRRQAQTTRKKAKKMVPKGQAHIYAAFNNTIVTITDTKGNTV
                     SWSSSGAAGFKGSRKSTPYAARLAGQNAARVAVDNGVQEVDVIIKGPGPGREAAIRSI
                     QASGIRVTSIQDITPVPHNGCRPPKKRRI"
     gene            complement(16496..16879)
                     /gene="rpsM"
                     /locus_tag="PLC06_00610"
     CDS             complement(16496..16879)
                     /gene="rpsM"
                     /locus_tag="PLC06_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRO88974.1"
                     /translation="MARIAGVDIPRNKRVEISLTYIYGIGRTSSHQILEQAEVNPDTR
                     VRDLSEAEVTRLRQLVEKEYTVEGDLRRETAMNIKRLGEIGSYRGLRHRRHLPARGQR
                     TRTNSRTRKGPKKTVAGRGRRKGGK"
     gene            complement(16892..17005)
                     /gene="rpmJ"
                     /locus_tag="PLC06_00615"
     CDS             complement(16892..17005)
                     /gene="rpmJ"
                     /locus_tag="PLC06_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRO88975.1"
                     /translation="MKVTSSVKRRCPKCKIIKRNGLVRVICSDPNHKQRQG"
     gene            complement(17047..17619)
                     /locus_tag="PLC06_00620"
     CDS             complement(17047..17619)
                     /locus_tag="PLC06_00620"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRO88976.1"
                     /translation="MRFIILIGPPGAGKGTQAKMLEKSLGLPQVSTGDLFRYNLKNET
                     ELGKLARTFMDKGELVPDEVTVAMVKDRLGQEDAANGAILDGFPRTQAQADALDKMLV
                     QLGGRITVVPNIVVEREELVQRLVKRSQIEGRSDDTEDTIRNRMRVYEEQTKPLLDYY
                     EQQGLVVDVDGQQSIEAVQQDLHRVIEQAH"
     gene            complement(17616..18986)
                     /gene="secY"
                     /locus_tag="PLC06_00625"
     CDS             complement(17616..18986)
                     /gene="secY"
                     /locus_tag="PLC06_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRO88977.1"
                     /translation="MIESVRAAFTLPDLRRRILYTIGMLIIYRLLANIPVPGVNLQAW
                     LLFTSQQTGNAVVDFLDLLSGGAVSNFSVMAMGVYPYITASIIIQLLTPIIPQLEELQ
                     SEGETGQNKLNRYTYYLTVPLAFLQAIGQIRLVGVSLTGGVESIMPNFGFGAAELLPT
                     LTTLIAMVGGTMFAIWIGERITEEGVGQGISLIIFGGIVAGIIPSLAQMLTLDTTARI
                     FSIFTFLLYLVLTVLVIVIIQEGQRRIAVQYGRRVRGRKIYQGQSTYIPLKVNTAGMI
                     PIIFAQSILTFFPLIAGLFMSGSGRFVDRVATAISQFGTNQDPTVAPFAFGAYWILYF
                     ILVVAFTFFYTDVMIRQQNLPETLQRQGGFIPGIRPGKRTESYIMAVVRRITLVGALF
                     LGLIAVLPGIMAVVAAVLQIDGLEQSVLVISGSGLIIVVGVVIDTMRQLEAQLLMRHY
                     EGFIKS"
     gene            complement(19041..19499)
                     /gene="rplO"
                     /locus_tag="PLC06_00630"
     CDS             complement(19041..19499)
                     /gene="rplO"
                     /locus_tag="PLC06_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003216888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRO88978.1"
                     /translation="MKLNDLGPNEGSKKSRKRVGRGISAGQGKTAGRGTKGQGARSGG
                     GKGAYFEGGQLPLARRLPFKRGFTNIRRVAYKAVNLDRLAEFEFGGETVDPDLMAAIG
                     LIKKPTDRVVVLGEGELAEAITVKAHRFSATAKEKIEAAGGKAEVIPFNA"
     gene            complement(19501..19713)
                     /gene="rpmD"
                     /locus_tag="PLC06_00635"
     CDS             complement(19501..19713)
                     /gene="rpmD"
                     /locus_tag="PLC06_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRO88979.1"
                     /translation="MTRLRITYSKSAIGYTVRQKATVKALGFKKLYETIEHEDSPAIR
                     GMINKISHLLTVEEVETGKSRSKKGE"
     gene            complement(19713..20246)
                     /gene="rpsE"
                     /locus_tag="PLC06_00640"
     CDS             complement(19713..20246)
                     /gene="rpsE"
                     /locus_tag="PLC06_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRO88980.1"
                     /translation="MGQRRQQSFREQKDDFDERIIDIARVAKVVQGGRRFAFRVTVVV
                     GDNRGKVGVGIGKARTVPDAIRKALETARKNMKDVPMYGTTLPHEVVGRHGAAKVLLK
                     PASPGTGVIAGGGVRAVIEAAGYRDILTKSLGSSNVLNVMMATMDGLDRLMDIRQVAI
                     NRGKDPKEVAPFWGRNK"
     gene            complement(20278..20640)
                     /gene="rplR"
                     /locus_tag="PLC06_00645"
     CDS             complement(20278..20640)
                     /gene="rplR"
                     /locus_tag="PLC06_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRO88981.1"
                     /translation="MAKNMNDPRKRRQRRIRLKISGTPERPRLNVFRSLEHIYAQVID
                     DVAGKTLVSASTVDKEVSEQVAGKKKREQATIVGKIVAQRALDAGIQEVVFDRGGYIY
                     HGRVKALADGAREGGLKL"
     gene            complement(20656..21198)
                     /gene="rplF"
                     /locus_tag="PLC06_00650"
     CDS             complement(20656..21198)
                     /gene="rplF"
                     /locus_tag="PLC06_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRO88982.1"
                     /translation="MSRVGKAPIQLPKGVSIDIDGTTVAVKGPKGELTRTLHPEMNIA
                     LEDGVLSVTRPSDDRQHRSLHGLTRALLNNMVTGVSNGYTKVLLIEGVGYRAEMDGKR
                     LVLYVGYSHPVYFEPDNGISFAVEDRGRKVIISGIDKEYVGEIAARVRKQRPPEPYKG
                     KGVRYEGEIVRRKAGKTGKV"
     gene            complement(21249..21656)
                     /gene="rpsH"
                     /locus_tag="PLC06_00655"
     CDS             complement(21249..21656)
                     /gene="rpsH"
                     /locus_tag="PLC06_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRO88983.1"
                     /translation="MSMTDPIADMLTRMRNALDRQQPSVAMPHSKLKVSIAEVLRNEG
                     YIESYEVVTQDKFPTLVIRLRYVGGRRDRRSVINGLQRVSSPGRRIYVGKKEIPWVLS
                     GLGVSILTTSRGVMTDQKARQLGLGGELLCKVW"
     gene            complement(21669..21851)
                     /locus_tag="PLC06_00660"
     CDS             complement(21669..21851)
                     /locus_tag="PLC06_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRO88984.1"
                     /translation="MAKKSMIARETKRKYRVRVRNRCQLCGRPRGYIRRFGLCRICFR
                     EQALKGNIPGVVKSSW"
     gene            complement(21877..22431)
                     /gene="rplE"
                     /locus_tag="PLC06_00665"
     CDS             complement(21877..22431)
                     /gene="rplE"
                     /locus_tag="PLC06_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRO88985.1"
                     /translation="MPLPQLYREEVVPALMKEFDYSSVMQAPKITKVVVNVGLGEALD
                     NAKAIEFATNDITIITGQKPVLNKAKQSIAGFKLREGRIIGLKVTLRGERMWAFLTRL
                     IHVALPRTRDFQGISPDAFDGRGNYTLGLREQLIFPEIDYDKIDKLRGMEVTIVTTAP
                     TDDEGRRLLALMGMPFRREVAEAA"
     gene            complement(22564..22887)
                     /gene="rplX"
                     /locus_tag="PLC06_00670"
     CDS             complement(22564..22887)
                     /gene="rplX"
                     /locus_tag="PLC06_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006700863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRO88986.1"
                     /translation="MRIKVNDTVEVISGNFKGVRGTVQRVYPKKQMVVVSGVNVIKKH
                     QKPQSASGRSRAQGGIIEFEAPIHVSNVMLVESGSGEKTRIGVRRDGNNHVVRYSKKS
                     GAELD"
     gene            complement(22922..23293)
                     /gene="rplN"
                     /locus_tag="PLC06_00675"
     CDS             complement(22922..23293)
                     /gene="rplN"
                     /locus_tag="PLC06_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRO88987.1"
                     /translation="MIQKESRLNVADNSGAREILVIQVMGGSRRRYGTIGDVVTATVK
                     KATPNATVKKSAVVKAVIVRTTKEYKREDGSYIRFDDNAAVILDAEGKNPVGTRIFGP
                     VARELRDKGFGRILSLAPESL"
     gene            complement(23307..23558)
                     /gene="rpsQ"
                     /locus_tag="PLC06_00680"
     CDS             complement(23307..23558)
                     /gene="rpsQ"
                     /locus_tag="PLC06_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRO88988.1"
                     /translation="MREQRKRLVGVVTSDKMDKTIIVSVAETMRHPIYGKVVSRVKKY
                     KAHDENNESQIGDRVLIIESRPYSREKHYALVSILERAD"
     gene            complement(23611..24027)
                     /gene="rpmC"
                     /locus_tag="PLC06_00685"
     CDS             complement(23611..24027)
                     /gene="rpmC"
                     /locus_tag="PLC06_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRO88989.1"
                     /translation="MANIVELNQMSNDKLEKTLEEAREEMFNLRFQVASARLENTARL
                     RQVRREVAQVETVLHQRQLATDAAAGEPEIAKRLEGAEWQANARYVYEDSAWQVEFND
                     KGGKKLATAWVNLNKARPKSRSDKQPKLVVRHELAR"
     gene            complement(24027..24443)
                     /gene="rplP"
                     /locus_tag="PLC06_00690"
     CDS             complement(24027..24443)
                     /gene="rplP"
                     /locus_tag="PLC06_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRO88990.1"
                     /translation="MLMPKRVKYRKQFRGRMRGMAKGGTTLLNGEYGLQALEPGWITS
                     RQIEAIRRTIVRQMRRRGKYWIRIFPDKPVTAKPAETRMGKGKGSVDHWVAVVKPGRI
                     IFEISGVPDDIAQEALRSAGFKLPIRTQVVAREEVL"
     gene            complement(24533..25198)
                     /gene="rpsC"
                     /locus_tag="PLC06_00695"
     CDS             complement(24533..25198)
                     /gene="rpsC"
                     /locus_tag="PLC06_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRO88991.1"
                     /translation="MGRKVHPVGFRLKSIRDWNARWFAEGGRYRELLHEDMMIRRVVK
                     KDLNQAGVAHIEIERFPNQVQVTIHTARPGIVIGRKGAAVKDLRQKLKDLTGKTVRVE
                     VEEITQPDANAQLIAENITGQLERRISHSRAMKRAVQQAMRAGVKGIRIEASGRLSGS
                     EMARNEKLWEGRVPRNMIRADLDYGFAEALTTYGRIGVKVWVYKGEILPQRVEATDVY
                     ISE"
     gene            complement(25241..25609)
                     /gene="rplV"
                     /locus_tag="PLC06_00700"
     CDS             complement(25241..25609)
                     /gene="rplV"
                     /locus_tag="PLC06_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRO88992.1"
                     /translation="MADTFEVKAIARHIPISTQKVRLVVDAVRGKAAGEALDALRFMP
                     QRSAEPIFKLIASAVANAEENYGLEMDELVVSRIWADEGPRHRKAPHGGRFAGRGRFR
                     PVVRRSSHITVVLAEREAAA"
     gene            complement(25612..25893)
                     /gene="rpsS"
                     /locus_tag="PLC06_00705"
     CDS             complement(25612..25893)
                     /gene="rpsS"
                     /locus_tag="PLC06_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRO88993.1"
                     /translation="MSRSLKKGPFIEPKLLKKVEDLNTARKRQVIRTWSRSSTIFPQM
                     VGHTIAVYDGRRHIPIYITENMVGHKLGEFAPTRTFRGHVAKSEKKGRK"
     gene            complement(25979..26818)
                     /gene="rplB"
                     /locus_tag="PLC06_00710"
     CDS             complement(25979..26818)
                     /gene="rplB"
                     /locus_tag="PLC06_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRO88994.1"
                     /translation="MPVKLFKPTSPGRRDMSGFTFEEITRTKPERSLVHGLRKRGGRN
                     VRGKITVRHHGGGHKRLYREIDFKRDKANIPARVATIEYDPNRSARIALLVYADGEKR
                     YILAPLGVKVGDTLVSGTKAEIRPGNSMPLRNIPLGTTIHNIELYAGRGGQLVRSAGV
                     SAQLMAKDHPQYATIRLPSGEERYVSQDCLATIGQVGNVEHGNIKLGKAGRNRHKGIR
                     PSVRGSVMSPRDHPHGGGEGTSPIGMAAPKTPWGKVALGKRTRNNKATDKYIFRRRGK
                     KRR"
     gene            complement(26842..27135)
                     /gene="rplW"
                     /locus_tag="PLC06_00715"
     CDS             complement(26842..27135)
                     /gene="rplW"
                     /locus_tag="PLC06_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRO88995.1"
                     /translation="MHWRDVIRRPVVTEKSNFLADVNNQYTFVVHPRANKMQVKQAIE
                     LAWPNVRVEKIRIANMPAKRARRVRRVTIRKSGWKKAMVTLKPGGRIDLFEGV"
     gene            complement(27137..27808)
                     /gene="rplD"
                     /locus_tag="PLC06_00720"
     CDS             complement(27137..27808)
                     /gene="rplD"
                     /locus_tag="PLC06_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRO88996.1"
                     /translation="MELPVYNMAGEQVSTFELPASIFEANINRDLMHQALVRQLANAR
                     LGTHKAKTRSEVNRTTAKAYRQKGTGRARHGSRKAPIFVGGGKAHGPLPRKYTKNMPR
                     KMRRAALCSALSVKADAGDIVLLDQISMDVPKTKSIAAMMKTLTQGETTLLLLADRNE
                     TVEKSARNLTDVKALRAGYLNIRDLLGYSKIVMPLAALDVVSSFLEQDMDDEWVDAED
                     AAVEG"
     gene            complement(27856..28551)
                     /gene="rplC"
                     /locus_tag="PLC06_00725"
     CDS             complement(27856..28551)
                     /gene="rplC"
                     /locus_tag="PLC06_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRO88997.1"
                     /translation="MRQLSSAGRLLVKGLIGKKVGMTQIFDDKGVVTPVTIIQAGPCF
                     VTQVKDNENDGYSAVQLGYGDVSPKRLTKGQQGHLGILKGEKKGRRKDKGIPAVRHLR
                     EFRTSSAGDYKVGQQLTVEQFIVGEKVDVTGKTKGRGFAGNVKRHGYAGGVKTHGQSD
                     RWRAPGSIGSATEMSRVPKGKSMAGRMGNERSTSQNLEVVRIDSERNLIAIKGAVPGA
                     KGGLVIIRDAAKG"
     gene            complement(28552..28860)
                     /gene="rpsJ"
                     /locus_tag="PLC06_00730"
     CDS             complement(28552..28860)
                     /gene="rpsJ"
                     /locus_tag="PLC06_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRO88998.1"
                     /translation="MSKQRIRIRLKAYDHRVLDQSAKRIVGTAERTGARVIGPVPLPT
                     RLERFTVRRSPFIDKDSQEHFEIRTHKRLIDVINPDSKTIDTLMRLNLPAGVDIEISL
                     "
BASE COUNT         5930 a         8883 c         7739 g         6185 t
ORIGIN      
        1 ggcaagggcg atcgcagagt gacaagccca tttgtgctat aatcccatac gattatgaga
       61 ctaaagaccc ccaaagaaat agtaaaattg cgagctgccg gtcgtctggt ggccgagtgc
      121 ttcgacatcc tgcaagaaaa catcaaacct ggcgtttccc tgaagaccct tgacaagatg
      181 gtcgaggacc atatctaccg gcttggcgcg gaaccactgt acaaaggcta tcgcggcagc
      241 gcccgagagc atcctccctt ccccggcgtc atctgcgcct cggtcaacca tgagatatgc
      301 cacggcctgc ccgacggccg cgttctggaa gagggcgacg tcattggtgt cgatatcggc
      361 ctgcggctga agggttactg tggcgatgcc tgcgtcactt accccgtcgg caaggtcgct
      421 ccccacgtgg aacgcttgct ggacatcggc cgcgaggccc tgcgcgtcgg cattgaggcg
      481 gcccaatacg acaaccacct caacgacatc ggtcgcgcca ttgaaggtta cgcccacacc
      541 cagggggtca cggttgtcca cgagtggggc ggtcatggca tcggccgcgt cctgcatgaa
      601 aatctgtccg tctcccacat caggcaatca agcccggggc cgcgactggt tccgggaatg
      661 accttcacca ttgagccaat gatcaatgag ggcacctacg agtggatcct gctgcccgac
      721 ggctggacgg tgatcaccgc tgacggcaaa ctatccgtcc agttcgagca caccatcgcc
      781 atcactcgca acggcccgga tatcctgacc cggctatagg aacccggaca cacgaacatt
      841 tgccggagaa caggcaagac aagccgcacc gaaattacct tgacttggtt gggtgtcaat
      901 caatggcggg atatcaaggt tgggggtgac tctggcgagc tgagtagtca cccccaaccg
      961 tcgtgtcaaa taacgatatt aacgagcgta ccgcgcacga caatcacttt gcggggctgt
     1021 ttgccgtcca acagacgctc gactttctcc gattcgaggg caatccgact taactgatca
     1081 tcatccatcc cggcgggcac atcgagtttg tcgcgggtct tgccgttgat ctggatgacc
     1141 aggctgatca tctcttcgcg cgccgcgtct tcgtcccact gcggccaaga ctgctgatgg
     1201 atgctatagg ggcggccgcg ctgcgaccat agctcttccg tgatatgagg cgagatggga
     1261 gccagcaaca ggaggatcgt gtccacggcc tcgtcccagg ctgcgcggct cgcctcggcg
     1321 gcgcgcagag cggccgacat cgtgtttcgc agctccatga tggccgccac cgccgtgttg
     1381 aaagagaact catccaggtc ctgcgtgacc ttgcgaatgg tttgatgagt cttgcgacgc
     1441 aaagcccgcg tggccgcttc atcctcatgc tcaggcgcat aatcagtgcg gccaatctcc
     1501 cagatgtcct gcagcaatcg ttgcggccct ttgatgccgc cgtaattcca cggcccgccc
     1561 tgatcccact tggcaaaaaa catcagatag gcgcgcacgg cgtccgcgcc gaactgggct
     1621 accaactcgt cgggattgac gacattgccc ttgctcttgg acattcgctg gatttccaga
     1681 tgatggcgca gctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgtcg
     1801 cccttgcgtt ccgcgcccag gatctgcccc tgattgcgca gcatggtcat cggctcgtca
     1861 aatgctcctc gcggatcgcg gtcatttgcg cgcatcatct gctccacctc ggcgaacata
     1921 cccagatcgc gcatggcctt ggtgaagtag cgcgtgtaga gcaaatgcat tgtggcgtgt
     1981 tcggcgccac cggtgtagac gtccaccggc agccagtaag cggcctcggc cggatcgaat
     2041 ggcccctgat catagcgagg gcttagatag cggtattgat accaggatga acacataaag
     2101 gtgtccatgg tgtccgtttc gcgtcgcgcc ggtcgccctt cggcgtcagt cgtgtggaga
     2161 aacgccgcgt gatacgtcag cgggctgcgg ccggtaggca tgaactcgac atcatcgggc
     2221 agcagcaccg gcaattcatc atccggcacc atctcaatcc cgtccggccc atagatcgcc
     2281 ggaatcggag cgccccaata gcgctgccgg ctgatgagcc agtcgcgcat ccgatagttg
     2341 accgacttgc tgccgatgcc tctctccgat agccaatcgc ccacggcgct gaccgaaggg
     2401 ttcgcccatc ccttggcact cgtcaccggt gtgccgtcaa actgtgcgct gttgatcatg
     2461 ctacccggcc ccacataagc cgcctccatc gtttcgccat ccaacgtttc gccttcaggc
     2521 tgaatgacgg ggatgatcgt cagaccatac ttgcgcgcaa aatcaaagtc gcgctggtca
     2581 tgggcgggca cggccatgat cgcgccggtg ccgtaggtgc tcaacacgta gtcggcgatc
     2641 cagaccggaa cacgttcctc attgaccgga ttgatggcat atccaccggt gaaaacaccg
     2701 gtcttctcgc gcgtgtcgga caatcgctcg atctccgtgg cgcgcgccgc ctgctcgcga
     2761 taggcctcaa ctgcttcgcg ctgcgtgtcg gaggtcagct tatccaccag gctatgctcc
     2821 ggcgacagga ccatgaatgt cgcgccccat agcgtatccg gccgggtcgt gaagatctcg
     2881 atggggtcgc cttgttcact catgaaaacg acgcgggcgc cttcgctgcg gccgatccag
     2941 ttggtttgca tgagcttgac cgggtcgggc caatcgagac gctcgaagtt gagcagctcg
     3001 tcacaataac gcgtgatggc gaagaaccac tgctccatga gcttctggac aaccggttgc
     3061 ccggtacgct cgtccttgcc gtcaattacc tgctcgttgg ccagcacggt ctgcaaggtc
     3121 tccgaccagt tgaccatacc ctcgccacga taagccaagc cgtttttata gaactgagag
     3181 aagaaccact cggtccaatg gtagtaggcc ggatcgcagc tcaccgcctc gcgttcccag
     3241 tcgaacatgg cacccatgct gcgcagctgc ttgcgcatcc gctcgatatt ggcgtaggtc
     3301 cacgtcttgg ggtgaatgtt gcgctggatg gcggcatttt ctgccggcag cccgaaggcg
     3361 tcgaacccca tcgggaacaa cacgttatag ccgcgcattc gcatgaaacg cgctcgcgca
     3421 tcagaagggg tcatggcgaa ccagtgaccg atatggagat ccccgctggg gtagggcagc
     3481 atggtcagcg cgtaatgttt tggcttattc caatcgacaa cggaacggta taacccatca
     3541 gcctcccact tctcttgcca tttaggctcc agtgccgccg gttcgtaact ttcgctcata
     3601 atcaggactc cacaatcacc aggataagtt cacttttcaa acaaaaaacc cctttcgctc
     3661 agagacgaaa ggggttccgc ggtaccactc tgattgacgg tacaaaggtg gacctgaagg
     3721 ggttcgaacc cttgacctcc acaatgccat tgtggcgctc tcccaattga gctacaggcc
     3781 cattaccatc cacttattac acgtaacggg tgtaatccga taccggctat tgtatcaccg
     3841 atatgactcc caggcgagtt cgacgccagt tgtcatcctt tcagccagcg ggatgactct
     3901 ctaaatgttt ccgcgtctac tactcctgat cgcagccttt atatcttcag ttgtaagtta
     3961 caggaggagc gcacgcgctc cggctgtcta atggacctga aggggctcga acccttggcc
     4021 tctacagtgc gattgtagcg ctctcccagc tgagctacag gcccaacagc aagacggaat
     4081 tctagcccag gtagcgcaca ttgtcaagcg gccgcaagcc tcgcccggcc ccaaatggct
     4141 cccccatttg acctaaacac cttcgttgcc tatcattgcc ggcaatacaa attagctgga
     4201 gatttgatga aaaagcacgt catcacgctt gaatattgcg ttccctgaaa ctattggtcg
     4261 cgtgccgccc gtgtggcgga cgacattatg tcaaattatc atcacgtgat tgaacgattt
     4321 accttgataa ccggttcgaa aggtgtcttc aacttcgtgg tggacggtca gacccttttt
     4381 tccaagaaag aagccggccg ccacgctgag ccgggcgaga tattgcgcct ctttcaggag
     4441 tatatcggcc cggacgtaga gccgtatccg caataatcac gcgccgaccg cttattcttc
     4501 ttcatagatg aagaacccgc gccccgtttt gcggccgagc atgtccgcgg cgaccattcg
     4561 gcgcaagagc ggcgtcggcc gatagcgatc gtcgccccat tcctcgaaca ggcccgtcat
     4621 cacgcccaac acggcgtcga ggccgatcat atccgcccag gccagcggac caatgggata
     4681 gttcatgccc aggcgcatcg ccttatcgat atcgtcggcc gaggcggctc cttccatcag
     4741 caagccggcg gcctcgttga tcaggcagca aaccgtccgc gctcgcacca gtcccggccc
     4801 atcagccacg acgatcgtct cctgacccaa cccgcgccag aacaactccg cctgcaccaa
     4861 ggccgattca tccgtctgca aggcgcttgt cagttcgatg ataccttcat caggtaccgg
     4921 cggcagcacg gcaaagccaa ccacgcgctc aggtcgcctt gtccacgaag cggccaacgt
     4981 ggcgctggtc gccagggaag acgtcaaaat caacgcgtcg tcgggtaata gagaactcag
     5041 cgccagcagc agctcttgct tggcggcggc cgactcgtta tgaagctcaa tggcgatctc
     5101 gatatccgcc agatggtcca tatcgaattc tgcctcaatt gccccgagga aatcttccac
     5161 caagaatacc cgcacatcat gagctgcccg attgcacaga cgctcaactt catcgacaaa
     5221 ggggatttcc ccggcaatca ataccttcat gttaagaggc tcctgnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnntcatt caaggcccat cgctcgacaa actgctcgcg
     5401 aatggcctct tgctccggct tggggaaaag ccgcttgcta agaaacgcac ccgtgcgtga
     5461 tgggccaaac agccgggcaa gcaaaagccg ctgttgaatg cgcaaccatt cgccaaagtt
     5521 ccgcgccacc agcgccgggc cgctattgac gatgaccatg ctgcgcaccc gttccggctt
     5581 gtcgacgacc aactggaagg cgatcatgcc acccatcgac aaaccaacaa tgtgggccga
     5641 ttcaattttc agagcatcga gcaatccgac gacatcgcct gccatcatcg atacgctata
     5701 ggggcctgcc ggcttatccg attcaccatg gccgcgcagg tcaaccgtga ccactcgata
     5761 atgcggggcg aaaaacggca cctggaatcc ccagtcttct ccctgggagc caagaccatg
     5821 caggaaaaga agaggctcac cctgcccttg ttcatcgtat ttgagattaa ttccgtttgt
     5881 atggagcgtc ggcatgttca ccctgcaggt ttgtctgaac aataacaagt gatggatact
     5941 cgtcgcgcaa ccagtccacc agggcggcga ttgcgcgatg aacgccctgc gtcggccgcg
     6001 ggaagcctac ctcagccatg gagatgacga tctcgccgct cgcccgcggc cggatttcca
     6061 ggccaatccg ctgcatcaac ggctcttcgt ggatataggt ctcgatcagt aatttgccgt
     6121 catggaagga ttgataagtc tctataaaac gaatccgggt cgattccacc gtcacaaccc
     6181 gcggacggaa ttggcggccg atctcctcca ccgtcgccgg cagccgaaaa acaacatgag
     6241 gcatcgataa ctccttggga atgaggaagc gttcaaccac cggcacagta ttgcgttgcc
     6301 acttgtaata acgagggctt cggccctcgt ccaaggcgac ctgctcatac ttggtccgaa
     6361 cgcgaccctc atcctctcgc tcaaatggtt gactcgtccg gctgtggaaa tagggcgtct
     6421 cccccaggct tgcggtgatc tgctcagcgt actcatcgtg atcggtggca atgtcgagaa
     6481 aggcctcagg catcatacga gaggcgagca aggcaagaaa ttccccatcg atgagccttc
     6541 gatccgtatg atcctttttc ggccatggat caggataatt aatgtaagcg gccgcgatgg
     6601 tgctcggctc acataatatt tgtaatgcgg agtgggcatc ggattgcagc aaacacacgt
     6661 tggtgagacc caatctctcc accttgcgca tggcattgcg gagagagggg atggatatct
     6721 ccaatcccaa gatattggca tcggggaatc tgcgggccag gtcgaccaaa aagaggccgc
     6781 tcccaaaccc gatttccagg acaagcggtg cgtttcggcc aaacaaggaa gcccaatccg
     6841 cgggccacga caactgctcg accttatacc aatcgacgaa agacatcttg tgcccattat
     6901 atgccggtgc gccattctcc gcacaatgac accacatccg gcgcgaggtg tggttgcgct
     6961 atcattccac atgcgccaca atacctgtct caacgaaact cactggacaa gatggtatgc
     7021 aacgtaaaat aaggattctg ggcgtgccca tggacctggg ccaaaatcga cgtggcgtgg
     7081 acacaggccc cagcgcggtt cgctacgcca acctgaagac caggctggaa gagctggggc
     7141 acatcgtaca cgatgaaggc aatatcgctg ttcccaaccc tgaagaacac gtggccgagg
     7201 ggacaggacg ccgcttgcag accatcacca ccatctgcca aacggtctac gaccgcgcct
     7261 gcccgtggat cgaggcgggc gagtatgcga tattcctggg cggcgatcat agcatcagca
     7321 tcggctctgt cgctgccgcc gcggcgcatc acgaaccgat aggcgtgata tggattgacg
     7381 cccattccga cttcaacacc cccgaaacgt cgccgagcgg gaacatccac ggcatgccgg
     7441 ttgccacgtt gattggcgaa gggcctgagc cgcttgtcag catcgggcat cccggcccca
     7501 aggtagacag cacccagatc gtccagatcg gcattcgcaa tctggacggg attgaacgcg
     7561 agcgcctcat ccatagcggc atccacgttt tcaccatgcg ccacgtggat gaactgggca
     7621 tggccgccgt cgcgcggcag gctctcgatc ggctgcacca tctcccgcgc attcacatca
     7681 gcctggatat ggacagcctc gatccatcgg aagcgccggg cgtgggcacg ccggtgccgg
     7741 gtgggctgac ctatcgcgag gctcatttgt tgatggagat cctgggggat agcgggcgag
     7801 tcgaatctct cgatatcgtc gaaatcaacc ccatcctgga cgagtcaaac aaaacggctg
     7861 aactggcggt cgaactggct gcctcgctac tcggccggcg aattctgtaa tagaggtcct
     7921 tctcaatggc agcactcaca cgcgggctgg gcgcggaaaa atatgaccgc atttacagcg
     7981 accatgatct ggtcgtccgc atcgttaatt atttcaaacc gtacaagaca aaagtcattg
     8041 tcatcgccat cgcgctattt acgattgccg cgatgggcgc actgctgccc atccttatcg
     8101 ccgatagttt gaactcaagc accgatggag gtgctttccc gctgcatctg gtcatcctgg
     8161 tctttgtcat tggcgtggcg acatgggtct tttcggccat tcggcgctat ttaaccgccc
     8221 aggtgatcgc cgacgttgtc atgaccatgc gcgacgacgc tttcaacgcg gcgatgaggc
     8281 aagacctgtc cttttttgat gaattcaaca cgggccgaat cgtcagccgc atcacgagcg
     8341 acacacagga cttcggcgaa gtcatcacga tcgctacgga cgtgatcaac caactgacgg
     8401 tagcgttcat tctcatcttc gttctgttca ctatcgattg gcggctgacc ctggccgtgc
     8461 tggcaatgac tccgcttgta gctgtcgtcg cccttgcctt tcgtcatctg gcccgagacg
     8521 tcacccgcca aagctcccgc gctttggcag aggtcaataa agccatccag gaagcggtca
     8581 gcggcattcg tgtggccaag aattttcggc aggaaggggc gatatacaac gagttcatgc
     8641 aggtcaacga tcaatcgtac cgcatcaaca ttcggcgcgc ctttgtgctg tccaatattt
     8701 tccccacgct gaacgccctg gcagggctgg gcacggccgc gctcgtctac tttggcggcc
     8761 gcagcgccgt tacaggggcg atcaccatcg cctcctggta tctgttcgtc accaccgtcg
     8821 atcggttctg gttcccggta acgaaccttt ccgccttttg gagtcagttc caggcaggat
     8881 tggcctccac ggaacgcgtg ttcggcctta tcgacgcgga ctccagcgtc gtccagaccg
     8941 ccgataagcc ggtgcattcg ctccggggcg aaattgtttt cgatcacgtc tcctttcgtt
     9001 actcaagtca ggaacaagtg ctcaacgact tcagcctgcg catccagccg ggcgaaagta
     9061 tcgctctggt cggtcatacc ggtgcgggca agtcgagcat catcaagctc gtggcgcggt
     9121 tttatgagtt ccaggaaggg acaatcaccg tggacggcac cgacatccgt gaattcgatc
     9181 tgaagagcta tcgcgacaag ttggggattg tctcccaggt acccttcctc tttgccggca
     9241 cagtcgccga caatattcgt tatgcctgcc catcggctac cgacgagcag attatgacgg
     9301 tcgcctgcaa gatcggcaac ggggagtggc tcgagacatt gcccgacggc atctacagcg
     9361 aggtaggcga gcgtggcagt cgcctgtcaa tgggccagag gcagctggtc gccctgaccc
     9421 gcgtcctcgt tcaggggccg agtatcttca tcctcgatga ggcgactgcc agcgtggacc
     9481 cgttcaccga atcgcaaatt cagcaggctc tggacctgat catggcgaac agcacagcca
     9541 tcatcatcgc tcaccgactg tccaccgtgc gatcggccga tcgcatcatc gttctgcaaa
     9601 aaggtcgaat tattgaggaa ggcagccact cgtggctcat gaaacagggc ggacactacg
     9661 ccgaactgta tgacacctac ttccgccatc agtcgccaca ggcgatccac acaccatcgt
     9721 ggatgcaaga acgctcacag agcgcatgat ggttccaccg gaataaagtg cctgtttact
     9781 ccaaagcagt cgtggatgtc tttgtcaggg ggccggctcg gctacactga ggaagtcacg
     9841 ggcaacccac ccctctacac cggcctcggt gcgcacctgt tgccacgaca agtcgtcggc
     9901 cgtttcctgc ccggccaata gcgtgacgac cgtgccgtcc aacaaccatt ccagttgctc
     9961 accggatgtg ctcggtgccc cacgcaacca gacacctacc gcgctactga cagcagccgt
    10021 aaccggcgcg gtcgttgctg ttggttcggc ggtaacctgg ggtgttgggg atgagggcgg
    10081 gctggtcggg gtcggctcaa ctgtcgcctg caccgcgggc tccgtggcta tagccgatgt
    10141 aggctcgatc tccaccgccg gtactgtcgg ggttgggttc ataatcgtcg cccccaaacc
    10201 cgagaagagc ggccgaaccg cgaagatgcc cagaacgatc agccccacga tgagtgaaaa
    10261 ggcggcgcga acccaataaa tctgactggt gcgtttggcc tccgcctgcc caacattata
    10321 ggcttgccgg tgaacgctgg atcgggcgcc cagtgccttc gacgcgaagt acagggccgc
    10381 cagcaccaac aaacctacca gcgataagag gacgatattc aatacttggt tcacggttct
    10441 tcaggcgaat cagggtcagg ctgactcatc gtcaacttta gcagctacat ctgaattcgc
    10501 aagggcagcc ttagcttctt cccataaagc gtccatttcg acaaacgaca gattgctcca
    10561 ttcaagaccg cgctcgacga tgaacttttc gaccagctgg aagcggcgca taaaacgggc
    10621 attggcctcg cgcaatgcga tttcagcctc aacccccagc cattgagcca ggttggtcat
    10681 gacaaacaaa acatcgccca gttcaagccg taactcttcg ggcgtggagg cctcgcgcag
    10741 ttcatccagc tcctcctcaa gcttctcata gacgccggag atatccggcc aatcgaagcc
    10801 tgtcttcgct gctctggact gaatcttttg agcgcgcatc aaagtgggca agctggaagg
    10861 aataccactc agcctggaga cgaattcatc ggctgcccga tcgcccttct ccatgcgctt
    10921 gataatctcc caattgcgaa gcacttcggc cgaatcgctg acctgccaat cgccccagac
    10981 atggggatga cgacgcttga gcttggcttc gatccccgcg atgacatcgg ttaaggtgaa
    11041 ctccccggcc tcggtcgcca tctgtgtctg catcacgatg tgatagagaa gatcgcccag
    11101 ctcctctctc aagttatcga catcctcggc atccagcgca tcgagaactt catacgcctc
    11161 ttcgagaaaa cccgtcctca tgctctgtgg cgtctgctcc tgatcccagg gacatccgcc
    11221 agggctgcgc agaacggcca ccgtctcggc cagcgcgggc aatccggcct tcagaggtaa
    11281 tggcggaaca tacagactag tgagatgatc gatataatcg cttcgatcga tctcatagag
    11341 gggagaccat tccacaagct ggtcttcctg accggcggag tgaatcaacg ccgccttatg
    11401 ctcggccgga tagatgccca tcagggagag cttcagctcg ctggccacca tgcggctata
    11461 tacctgcccc agtaatagag gcacgtcggg gttaagcggt gggtacaggg ctgaagcgat
    11521 ctcgataccg tcgaatatct gcagaccatc aagggcatca atccccatcg ccgccagagc
    11581 cggctcgaca aaactaatgc ccggcacaat gcgaacgggt ataccggcgt tcgccgcgcc
    11641 tctcaggatc gttgcgaccg ttgtctcgcc aacgaacggg ttaccgggca ctgcatagag
    11701 gatatctttc tcaatgccta tacgaagcac ttcttctgcg atttgtcgat aaacctcgtc
    11761 gaatcgctcg gccgtctgat agatctcatc gaaagcatgg cgtttgatgc gggcggggag
    11821 gccgtccacg gccgggtggc gggccgtgcg aagataaacg gtgtcggccg cagaaaggag
    11881 atgccaggcc tgaatggtga tcaggccggc atctccgggt ccgagaccaa caatggtgat
    11941 ggtcatgggt gaagagctgc gccaatcggc atgacggcgc agctcaataa tgccttaaac
    12001 aggcgacgct taacgcttgg tgtaggtcgg agccttgcgg gcacgcttga ggccgggctt
    12061 tttgcgttcc ttcacgcgcg cgtcccgtgt cagatgatct ccggcgcgaa gcggtgtacg
    12121 caaattctcg tcatacttga cgagcgcacg agccaggcca aggcgaacgg cgctggtctg
    12181 gccggtgatg ccaccgccct gaaccaacac ggtcacgtcg accttaccca tgaggccgct
    12241 atcttccaac gggccgatca acgtctggta atcgccatag cgcgggaaga actcctgcac
    12301 aggcttttcg ttgacgatga acgaaggacc ttcctcctga ctcgcctcac ggaagtagat
    12361 gcgcacgcgc gcggaggcct ctttacgacg cccaattccc tcgaaatatt cgctcataat
    12421 tgattcctct taacctggtg aataccgggt ttctacagct ccaacggctg cggctgctgc
    12481 gcctggtgag gatgttccgt accggcgtat accttcagct tcttgaacat cttgcggccg
    12541 agcgtattct tcggcaacat ccctcgaacc gcagactcga ccacgcgctc cgggtgctgc
    12601 tgcaattgat cgcgcagggt gatctctttc aggccaccga tatagagcga atggcggtaa
    12661 tacttcttct gctccaggcg gcggccggtc acagcgatct tgtcggcgtt cacgacaacc
    12721 acgaagtcgc cgcagtccac cgcagggctg taagtcggct tgtgcttgcc cttcaggatc
    12781 gtcgccacgc tcgacgccag gcgacccagg gtctggccct cggcgtccac aacgtaccaa
    12841 gcgcgctcta tgtccgcagg tttggttacg taagtcttca tctcattcct ccaccggcgg
    12901 gcggccggat attcaggtcc tcattcatta tcgtattcaa cagataccag gtaaagcccg
    12961 tgggccgggg ccgccgtcgc cgagcgcttt cgctctcgcg cagccagcgc gtccgcaaaa
    13021 tcgcccaccg accatttacc gcagccgacc gctttaagtg accccacgat gctgcgcacc
    13081 attcgataca gaaagccatt ggcgctgatt tcgaaggtca ccatctgtgc atcgcgtcgc
    13141 catacggccg agtacacttc gcgtatcgtg ctctcgccaa cggttggccg gccgaatgtg
    13201 gcgaaatcgt gcaccccaat gagcaaggcg gccgcttcgt tcattcgctc cggatccaat
    13261 cgctgagcaa catgccaggc gcggtgacgc cataacggct gtcgaatcgg cacattcagg
    13321 atatggtaca cgtaagatcg ccggcgggcg tcgaaccgtg gatgaaacct cggttgcgcc
    13381 atcgccaact cgctaatgac gatatcctgt ggcagattcg cgttcagcgc tcgatgcaac
    13441 gcctcagacc catgcttttc cggccactca attgtaaacc ctatgacctg tcccagcgcg
    13501 tgaacgccgg tatctgtccg ccccgcccca agcacccgta ccgggtgatc gacaagtcgg
    13561 gccaaggcat gttcaagctc ggcctgaacc gaaggaactc cgcgtcgctg tcgttgaaac
    13621 ccataataat tggttccatc gtactgcaca gttgcccgat aagtgcgggc atcgacgcta
    13681 tgtttctcct tcggctcaaa cgtcacatga atcgtttagt cttcgaccag catcagcacg
    13741 gccatttcgg cgttgtcgcc gggccgccgg ccaatcttca ccatgcgagt gtagccgccc
    13801 gggcggttcg cgtatcgggg agcgatctcc tcgaacagct tgcgcacaac gtctacatct
    13861 tcgaaggtgc cgtcttccgc ttcggtcgtg cgcgtgttgg gcaggcgcga ggccgccaac
    13921 cggcgcgcat gaatcgcgga acccggctgc tcatcagact gggccaaacc acgcttggcc
    13981 agggtgatca tcttctccgc ggccgggcga atggttcgcg ctttggcctc ggtcgtcgta
    14041 atttgttcga ataccagcag atcggcgatc aggttacgcc gtagcgcgtt gcggtgggcg
    14101 gtatcgcgat ttagcctgcg gccaaatact ttatgacgca tcgttaactc ctctattagc
    14161 catcggaggc ttaggccgac cgcttggcct catcttcttc cggtaggaaa ccctttaacc
    14221 gaagctgttg tttcaactca tccagcgact tgtcgccgaa gttacgaatg gccagcatcg
    14281 tctcttcgcc acggtccagc atttccagca tctcgccaac cttggtgatg ccggtgcgct
    14341 tcagagagtt aaagacgcga acgctgagat cgagttgctc gatcggtgtg tcgtaaattt
    14401 cgttggggat cgtctcctct tctctctctt cctcgacggt gacaaaggtc tcttcgctga
    14461 cgccggcaat tggccggaga tgctggatca ggatttgcgc ggcctgagcc aacgcctctt
    14521 caggcttgat cgcgccgtcg gtccatattt ccatgacggt gcgatcataa tcggccgcct
    14581 gaccaacacg ggtcttttcg acttcgtagg ctacgcggcg gatcgggctg aagatcgagt
    14641 cgaccggcaa ctcaccgatc ggcaagcggc cacggtcctc ggcgggcgag tagccgcgtc
    14701 cggtctccgc catcagttcg atctcaagaa atgtatcatc ggcgtcaacc gtaaacagat
    14761 acagatcagg attaatgatc tccacctcgg gcggaacctg aatgtcggcc gcggtaacgg
    14821 ggccggcacc ctgcacctca aggcgcatac gggcaatatc cgtattcacg agacgcagcc
    14881 gcagctgctt cacgtgcaaa ataaactgca tcatatcttc gcgcacgccg gggatggccg
    14941 aaaactcatg cggcacgtcg gtcacgcgaa ttgatgtaat ggccgcaccg ggcaacgacg
    15001 ccagcagcac gcgccgcagc gcattgccga ccgtggtgcc atatccccgc tccagcggcc
    15061 cgatcacgaa gcggccatat tcagcagtca tggccgtgct ttcgattttc ggcattacaa
    15121 gattattgat agccaaggcg attacctccc tctcgggaat ccgcgggcgg gatcgatccg
    15181 ttcgagaccg ctcccgccgc gcttgtcaac tcccataaat cggtctgttt caagataagc
    15241 aactgccgtt aacgggagta gtactcgaca atcagctgtt cgttgagcga gatgtcgata
    15301 tcttcgcggg caggcgcgtg catgacgcgt cccgacagat tggcggtatc gaccgtcaac
    15361 cagcgcggta cgcggcgatc atccatattt tgccgcaggt ccttgaagta agtccgccgg
    15421 gtgctctcct tgcggacgga cacgacatca ccgggggcta ccagggccga cgggatattg
    15481 gtcttgcggc cgttgagcat gatgtgacca tgctggacca actggcgagc ctgagcgcgc
    15541 gagtcagcca gacccaggcg atagaccacg ttatccaggc gacgctcaag aatggccaac
    15601 aggttataac cggtcagacc cgcctggcgg gaggcgcgct caaagtaacg gctgaactga
    15661 cgttccagaa cgccgtagat acgacgagcc ttctgcttct cgcgcaactg caacagatag
    15721 tcggatgcgc ggccacggcg gaactgattc tcccgcccgt gctgccccgg cggatagcca
    15781 cggcgctcga ctgaacattt gggggtcaga caacgcgacc ccttcaaaaa gagcttttcg
    15841 ccctcgcgcc ggcacaaccg gcatacaggt ccactataac gtgccaattt aggtacctcc
    15901 tattgagtac cgagcgcggc ggcaaatgta tcgccccgcg gccaggattg ggagaggttt
    15961 acgggcttgg ctacccttcg tccctctcag catcaccaat gaatgattaa attaaatacg
    16021 acgtttcttc ggcggccggc aaccattgtg gggcacgggc gtgatgtcct gaatcgaggt
    16081 cacgcggatg ccgctggcct gaatcgaacg aattgccgcc tcacggcccg ggccggggcc
    16141 tttaatgatg acgtctactt cctgcacgcc attatcaacc gccacgcggg cggcgttctg
    16201 gccggccaga cgagcggcat agggcgtgct cttgcgtgag cccttgaagc cggccgcgcc
    16261 tgagctggac caggaaacgg tgttgccctt ggtatcggtg atcgtcacga tggtgttatt
    16321 gaaggcggca taaatatgag cctgcccctt tggcaccatc ttcttggctt tcttacgcgt
    16381 cgtttgtgct tgtcttctac gtcccatcgt tcctctatga ttaacccaac agcggacgac
    16441 gcgccggcaa cgataaatcg ttacccggca cgtcgtccgt cactcattat ttcaactact
    16501 tgccgccctt acgacgacca cgcccggcaa ccgtcttctt cggccccttg cgggtgcgcg
    16561 aattggtgcg cgtgcgctgc ccacgagcgg gcagatgccg gcgatgacgc aggccgcgat
    16621 agctgccgat ttcgcccagg cgcttgatgt tcattgccgt ttcgcggcgc agatcacctt
    16681 caaccgtgta ctctttctcg accaattgac gcaagcgcgt gacttcggct tcagacagat
    16741 cgcgaactcg ggtatccggg ttcacttcgg cctgctcgag aatttggtgg ctggatgtcc
    16801 ggccgatccc gtaaatatac gtcagactga tctctacgcg cttgttgcgc gggatgtcga
    16861 cacctgcaat acgagccatt ctgtctctcc cttaaccttg acgctgcttg tgattcggat
    16921 cagaacagat cacgcgcacc aggccatttc gcttaatgat cttgcatttc ggacaacgcc
    16981 gcttgacaga tgatgtaact ttcatgattc cactctccaa tgtcgggcga tatcccgacc
    17041 attctatcaa tgagcctgct cgatcacccg gtgcaggtcc tgctgaacag cctcaatact
    17101 ttgctggccg tccacatcca cgactaaacc ctgctgttca taatagtcca gcagcggctt
    17161 ggtctgttct tcataaacgc gcatccgatt gcggattgta tcttccgtat catcggagcg
    17221 cccttcaatc tgcgagcgct tgacgaggcg ctggaccagt tcttcccgct cgaccacaat
    17281 gttggggacg acggtgatcc gaccgcccaa ttgtaccaac attttgtcaa gtgcgtcagc
    17341 ctgggcctgg gttcgcggga atccatcgag gattgccccg ttggccgcgt cttcctgacc
    17401 cagacgatcc ttcaccatcg ccacggtgac ttcgtcggga accagctcgc ccttatccat
    17461 aaaggtgcgg gcgagcttgc ccaactcggt ctcgttctta agattgtaac gaaacaaatc
    17521 gcctgtcgaa acctggggca gccccaggga tttctccaac atcttggcct gagtgccttt
    17581 tccggctccc ggcgggccaa tcaggatgat gaacctcatg acttgatgaa cccttcatag
    17641 tggcgcatca acagttgggc ttccaactgg cgcatcgtat cgatgacgac acccaccacg
    17701 atgatgagtc ccgaaccact gataaccagc acgctctgct ccagaccgtc gatttgcagc
    17761 accgctgcaa caaccgccat aatgccgggc aatacagcga tgagacccag gaacagcgcg
    17821 ccgaccagtg taatacggcg cacaacggcc atgatgtagc tctcggttcg cttgccgggg
    17881 cgaatgccgg ggatgaaacc accctggcgc tgcaacgtct ccggcagatt ctgctggcga
    17941 atcatcacgt cggtgtagaa gaacgtgaag gctacaacca gaatgaagta cagaatccag
    18001 taggccccaa aggcaaacgg cgcgacagtc gggtcctgat tggtgccgaa ctggctgatt
    18061 gccgtcgcca ctcgatcgac gaagcgcccg ctgccactca tgaacagacc ggcaatgagc
    18121 gggaagaacg tcaggatcga ttgggcaaag ataatcggga tcatacccgc ggtgttaacc
    18181 ttcagcggta tataggtgct ctgaccctga taaatcttac gtccacgcac gcggcggcca
    18241 tattgcacgg caattcgcct ctgcccttcc tggatgataa cgatgaccag caccgttagc
    18301 accagataga gcaggaaggt gaaaatcgag aagattcgcg ccgtcgtgtc caacgtcagc
    18361 atctgggcca gactgggaat aatgccggca acgataccgc caaagatgat caaggagata
    18421 ccctggccga cgccttcttc cgtgatccgc tcgccaatcc agatagcgaa cattgtgccg
    18481 ccgaccatcg cgataagtgt ggtcagtgtc ggcaatagtt ccgctgcgcc aaacccaaag
    18541 ttgggcataa tcgactcgac gccaccggtc aggctgacac ctaccagacg aatctggccg
    18601 atcgcctgca ggaacgccaa cggcacggtc agataatagg tataacgatt cagcttgttc
    18661 tggccggtct cgccctcaga ctgcaactct tcaagctgcg gaatgatcgg cgtgagcaac
    18721 tgaatgataa tcgacgcggt gatgtagggg taaacgccca tggccatcac cgagaagttg
    18781 ctgaccgcgc cgcccgacag aaggtcgagg aagtcaacta ccgcgttacc ggtttgctgc
    18841 gatgtaaaca gcaaccaggc ttgcaggttc acccccggaa cggggatgtt tgccagcaag
    18901 cgatagatga tcagcatccc gatggtatac aggatgcgac gcctcaggtc aggcaaggta
    18961 aaggctgcgc gtactgattc tatcattaca tttctccggc acgctcaaat gccacccttc
    19021 gttcggcaca ggccgaccga ttaggcattg aacgggataa cctctgcctt gccacccgcg
    19081 gcctcgatct tctctttggc cgtggctgaa aagcgatgcg ccttcacggt gatcgcttcg
    19141 gccagttcgc cttcgcccag gacaacaacc cgatccgtgg gcttcttgat cagaccaatg
    19201 gccgccatca aatcgggatc aaccgtttcg ccgccgaatt caaactctgc caaccgatcg
    19261 aggttgacgg ctttgtatgc cacgcggcga atgttggtga atccgcgctt gaacggcaag
    19321 cgccgcgcca acggcagctg gccgccttca aagtaggcgc ccttgccgcc gccgctgcgc
    19381 gcaccctggc ccttcgttcc gcggccggca gttttgcctt gaccggcgga gatgccgcgg
    19441 cccacgcgct tgcgcgattt cttcgagcct tcgtttggac ccaaatcgtt gagtttcatc
    19501 tcactcgccc ttcttggacc gcgatttacc ggtctcaact tcttcaaccg tcaacaggtg
    19561 gctgatcttg ttgatcatcc cccgaatcgc cggcgaatcc tcgtgttcga tcgtctcgta
    19621 caacttctta aagccaagag ccttcacagt ggccttttgg cggacggtgt acccgatcgc
    19681 gcttttgcta taggtgatgc gtaacctggt cattacttat tacgccccca gaacggcgcc
    19741 acttctttcg gatctttgcc ccggttgatc gccacctggc gaatgtccat cagccgatcc
    19801 agaccatcca tggtcgccat catcacgttg agcacattcg aactgcccag cgacttcgtc
    19861 agaatatcgc ggtagcccgc cgcctcgatc acggcgcgaa cgccaccacc ggcgataacg
    19921 ccggtaccgg gggaggccgg cttgagcagc accttggcgg cgccatgccg accgactacc
    19981 tcgtgaggca gcgtcgtgcc atacatcggc acatccttca tattcttccg cgcggtctcc
    20041 agcgccttgc gaatcgcatc gggcaccgtg cgggccttgc cgattcccac gccgaccttg
    20101 ccgcggttgt cgccaaccac aacggtgaca cggaaggcga accgacggcc gccctgcaca
    20161 accttggcta cacgggcgat gtcgatgatg cgctcgtcga aatcgtcttt ctgctcgcga
    20221 aagctttgtt gtcttctctg acccatcgtt gactcctgaa cgggcaatcg ccccgtccta
    20281 cagtttcagg ccgccctctc gcgcgccatc agccagggcc ttcacacggc catggtaaat
    20341 atagccgcct cgatcgaaga cgacttcctg aattcccgcg tcgagagcgc gctgggcaac
    20401 gatctttcca acgatcgttg cttgctctcg cttcttcttg ccggccacct gctcgctcac
    20461 ttccttgtcc acggtagacg ccgagaccag ggttttcccg gccacgtcgt cgatcacctg
    20521 ggcatagatg tgctccaggc tgcgaaagac gttcagacgc ggacgctcgg gtgtgccgga
    20581 gatcttcagc cggatgcgac gctggcgacg cttccgcgga tcattcatgt tctttgccat
    20641 aatgtcccta tcctattaaa ccttaccggt cttaccagcc ttgcgtcgaa cgatttcgcc
    20701 ttcatagcga acgcccttgc ccttataagg ctccgggggc cgctgcttgc gcacgcgagc
    20761 cgcaatttcg ccgacgtatt ccttatcgat accgctgatg atcaccttgc ggccgcggtc
    20821 ttccacggca aaggatattc cgttgtccgg ctcaaagtaa accgggtggg aatagcccac
    20881 atagagcacc agccgcttgc cgtccatctc ggcgcgatag ccgacgcctt cgatgagcag
    20941 caccttggtg tagccattgc tgacacccgt gaccatattg tttaacaacg cccgtgttaa
    21001 cccgtgcaat gagcgatgct gccggtcgtc ggacggccgg gtgacgctca ggacaccatc
    21061 ctcgagcgcg atgttcatct ccgggtgaag ggtgcgcgtg agttcgccct tcggaccttt
    21121 caccgcgacg gtcgtgccat caatatcgat gctcacgccc ttgggcagtt gtattggcgc
    21181 cttaccaact cgagacatac caaatactcc ctggcggacg aatgtccgcc atcttgtgtc
    21241 gaatcttcct accagacctt gcagagcaat tcgccaccca aaccgagctg gcgggccttc
    21301 tggtcggtca tgacgcctcg tgacgtagtc aagatcgaga caccaaggcc actcagaacc
    21361 cagggaatct cctttttgcc cacgtagatg cgacggcccg gtgagctaac acgctgcagg
    21421 ccgttgatga ccgagcgacg atcgcggcgg ccgcctacgt agcgcaagcg aatgaccagg
    21481 gtcgggaact tgtcctgcgt cacaacttca taactctcga tgtagccctc gttcctcaga
    21541 acctcggcga tcgagacttt caatttcgaa tggggcatgg cgaccgacgg ctgctgcctg
    21601 tctagggcat tacgcatccg cgtcaacatg tcggcaatcg gatcagtcat tgacatttcg
    21661 cacctctact accaactcga tttgacgacg ccgggaatat tccctttcaa ggcctgttcg
    21721 cggaagcaaa tgcgacacag tccgaagcga cggatatatc cacgcgggcg gccgcacaac
    21781 tggcagcggt tacggacacg aacccgatat ttccgcttcg tctcacgtgc gatcattgat
    21841 ttcttcgcca tctatattct ccagagcgct tagcgcttag gctgcttcag cgacttcgcg
    21901 cctaaaaggc attcccataa gcgccaacag tcgtcgacct tcatcatcgg ttggggcggt
    21961 ggtgacaatg gtcacttcca tgccccgaag tttatcgatc ttgtcgtaat cgatctccgg
    22021 aaagatcaac tgctcgcgca ggcccagggt gtaattgccc cgaccgtcaa acgcgtcggg
    22081 cgaaattccc tggaagtcac gggtacgcgg caaggcgaca tggatcaggc gggtcaaaaa
    22141 cgcccacatc cgctctcctc gcagggtcac cttcagaccg atgatgcgac cttcgcgcag
    22201 tttgaaaccg gcgatcgact gcttggcctt gttcagcaca ggcttctggc cagttattat
    22261 cgtgatatca tttgttgcga actcgatcgc cttcgcgtta tcgagtgcct cgcccaaacc
    22321 cacattgacc acgaccttgg taatcttcgg cgcctgcatg acactggagt aatcgaactc
    22381 cttcatgagc gccggaacta cctcttcgcg atacaactgg ggcagcggca taaaattggt
    22441 tgccattgtt gtttctccgg gtcgagtagc aggtggccac ggttcccgcc tgagacgcat
    22501 atagcgcgtc tcggttcagg ggccgcttgg gctgcctggc tcgcctattt taaattcctg
    22561 ctgttaatcc agctccgcgc ctgacttctt cgagtagcgc acgacgtggt tattcccgtc
    22621 gcgacgcaca ccgatgcgcg tcttctcgcc gctgcccgat tcaacgagca tcacgttgga
    22681 cacgtggata ggcgcttcga actcgatgat gccaccctgt gcccgcgaac ggccgctggc
    22741 cgactgtggc ttctgatgct tcttgataac gttgaccccg gaaacgacaa ccatttgctt
    22801 cttggggtag acacgctgga cggtgccgcg cactccctta aagttcccgg agataacttc
    22861 taccgtatcg tttaccttaa ttcgcatgac ttaccttatc cagtccctgt aatccaatcg
    22921 gttacagaga ttccggcgcc agggacagaa tacgcccaaa acctttgtca cgcagctcgc
    22981 gagccaccgg cccgaaaatg cgtgtgccga caggattctt gccctcggca tccaggatga
    23041 ccgccgcatt atcatcgaaa cggatgtagc tgccgtcttc ccgcttgtat tccttggtgg
    23101 tgcgcacgat gacggccttg accacggcgc tcttcttcac ggttgcgttg ggcgtcgctt
    23161 tcttgacagt cgcggttacg acatcgccga tggtgccata acggcgccgc gatccaccca
    23221 tcacctgaat caccaggatt tcgcgggccc ccgaattatc ggctacattg agacgacttt
    23281 ccttctgaat cattgggatg cctcgcttag tccgcgcgct ccagaatgga gacaagcgca
    23341 tagtgctttt cccggctata aggacgggat tcgattatta agacccgatc gccaatctgg
    23401 ctctcgttat tctcgtcgtg ggccttgtac tttttcacgc ggctgacaac cttgccataa
    23461 attggatggc gcatggtctc ggccacggag acaatgatgg tcttatccat cttgtcgctg
    23521 gtcacaactc ccacgagacg cttacgttgc tctctcatct tgattacctc gtgttgcctg
    23581 gcgtagcgca attgctagcg agcgcgatcc ttatcgcgcc agctcatggc gaacgaccaa
    23641 tttcggctgc ttgtccgaac gactcttggg gcgcgccttg ttcaggttca cccacgcagt
    23701 cgccagcttt ttgccgccct tgtcattaaa ctcaacttgc cacgcgctat cctcatagac
    23761 atagcgggca ttggcctgcc attcagcgcc ttcgaggcgc ttggcaattt ccggctcgcc
    23821 cgcggccgcg tcggttgcca gttgtcgctg atgcaaaact gtctctacct gggcgacctc
    23881 gcggcgcacc tggcgcaagc gggcagtatt ttccaatcga gccgaggcaa cctggaagcg
    23941 cagattgaac atctcttcgc gcgcttcttc gagcgtcttc tcgagcttat cgttgctcat
    24001 ctgattgagt tcgacgatgt tggccattag aggacctcct cgcgagcgac gacctgcgta
    24061 cgaatcggca gtttaaagcc ggccgagcgc aaggcctcct gggcgatgtc gtcgggcaca
    24121 ccggaaatct cgaagataat ccggcccggc ttaacgactg ccacccaatg atcgaccgaa
    24181 cccttgccct tacccatgcg ggtctccgcc ggcttggcgg taacgggctt gtccggaaaa
    24241 atacgaatcc agtatttgcc acgacggcgc atctggcgaa cgattgtccg gcgaattgcc
    24301 tcgatctgac ggctggtgat ccagccgggt tcgagcgcct gcaaaccgta ctcgccattt
    24361 agcagcgtgg taccgccctt ggccatgccc cgcatgcggc cccggaactg cttgcgatac
    24421 ttaacgcgtt tgggcattaa catgtgactt tctccgctag ttactcagtg tgcgctattt
    24481 cgtgacatcg aacgcgagcc gcagcccgtc gctcatccgt ttccgatcac gcttactcgc
    24541 taatatatac gtccgtcgcc tcaacgcgct gcggcaggat ctcacccttg tagacccata
    24601 ccttcacgcc gatgcggccg taggtcgtta atgcttcggc aaagccatag tccagatcag
    24661 cgcggatcat attacgcggc acacggcctt cccacagctt ttcgttgcga gccatttccg
    24721 aaccggacag acgaccactc gcctcgatgc gaatgccctt caccccggcg cgcattgcct
    24781 gttgaacggc gcgcttcatg gcccgactgt gcgaaatacg acgctccagt tgcccggtga
    24841 tattttcggc aatcaattgg gcgttggcgt cgggttgggt gatctcctca acctcgaccc
    24901 gcaccgtctt gccggtcagg tccttcagct tttggcgcaa atccttcacg gccgcgccct
    24961 ttcggccaat tacgatgccg gggcgagccg tatggatcgt gacctgtacc tgatttggaa
    25021 agcgctcgat ctcgatatgc gccacacccg cctgattcag gtcctttttg accacgcggc
    25081 ggatcatcat atcttcgtgc agcaactcgc gatagcggcc accctcggca aaccaacggg
    25141 cgttccaatc acggattgac ttcaaacgaa agcctactgg gtgtacttta cgtcccactt
    25201 aatcctccat ccgggtatcc cggatcagtt tgcaatggcc ttaggcggcc gcctcgcgct
    25261 cggccaatac gaccgtgatg tgcgatgaac gacggacgac ggggcgaaac cggccacgcc
    25321 cggcaaagcg gccaccatga ggcgctttcc gatggcgcgg gccttcgtcg gcccaaattc
    25381 ggcttaccac caattcgtcc atttccaatc catagttctc ttcggcattg gcaacggccg
    25441 aagcgatcag cttaaatatc ggctcggccg agcgctgggg catgaatcgc agcgcgtcca
    25501 atgcctcacc ggcagccttg ccgcgaaccg catctaccac gaggcgcacc ttttgcgtcg
    25561 agatcgggat gtggcgagca atcgctttta cttcaaatgt atcggccatg attacttacg
    25621 ccccttcttc tcggatttgg ccacatggcc gcggaacgtg cgtgtcggcg cgaattcacc
    25681 cagcttgtgc cccaccatat tctcggtgat gtaaatcgga atatggcgac gaccatcata
    25741 tacggcgatc gtgtgaccca ccatctgtgg aaagatggtg ctgctgcgcg accacgtgcg
    25801 aatgacctgg cgcttgcgcg cggtattcag gtcctcaacc ttcttcagaa gtttcggctc
    25861 gataaatggc cctttcttca atgaacgtga catgttcaat ctccagaact acgaactacg
    25921 atctacaaac gtgatacgaa agatttgctc tgaattcgtc gttcgcaatt cgtaattcct
    25981 aacgacgctt cttgccccga cgacggaaga tatatttatc cgtcgccttg ttattacgcg
    26041 tccgcttgcc cagagcaacc ttgccccagg gcgtcttggg cgccgccata ccaatcgggg
    26101 aagttccttc gccaccacca tgaggatggt cgcgcgggct catcacagat ccacgcaccg
    26161 aaggccgaat acccttgtgc cgattgcggc ccgccttgcc cagcttgata ttgccgtgct
    26221 cgacgttacc gacctggccg atggtggcca ggcaatcttg cgatacatat cgctcttcgc
    26281 cggatggcag gcgaatcgtc gcgtattgcg ggtggtcctt ggccatcaac tgagccgaga
    26341 caccggcgga acgaaccaac tggccaccgc ggccggcata caactcgata ttgtgaatcg
    26401 tcgtgccgag cgggatattg cgcaaaggca tgctattgcc cgggcgaatc tccgccttcg
    26461 ttccactcac aagcgtatcg ccaaccttga cgcccaatgg ggccaggata tagcgctttt
    26521 cgccgtcggc atacacgagc aacgcgatgc gcgccgagcg attcggatcg tattcaatcg
    26581 tcgcaacacg ggccgggatg ttggccttgt cacgtttgaa atcgatctcg cgatagagtc
    26641 gcttgtggcc gccgccatga tggcgaacgg tgatcttgcc acgcacattg cggccaccgc
    26701 gcttgcgcag tccgtgtacc aggcttcgct ccggcttcgt gcgggtgatt tcttcgaacg
    26761 taaagccact catatcacgg cggccgggcg aagttggctt aaacagtttt acaggcatga
    26821 ttcctccagt caggcgccaa cttatacgcc ttcaaacaaa tcgatgcggc cacccggctt
    26881 aagagtgacc attgccttct tccacccgct cttgcgaata gtcacgcggc gaacccgacg
    26941 tgcccgcttg gcaggcatat tggcaatgcg aatcttttcc acacgcacgt tgggccaggc
    27001 caattcaatg gcctgtttca cctgcatttt gttcgcgcgc gggtgaacaa caaatgtata
    27061 ttgattattg acgtcggcca gaaagttgct tttctcggta accaccggcc ggcggataac
    27121 atcgcgccaa tgcatcttac ccctccaccg cagcgtcttc ggcgtctacc cattcgtcgt
    27181 ccatgtcctg ttcgaggaag ctggagacga cgtcgagcgc cgctagaggc attactattt
    27241 tgctatatcc caaaagatcg cgaatgttca ggtagccggc tcgcagggcc ttgacatccg
    27301 tgaggttgcg cgccgacttc tcgaccgttt cattgcgatc agccaggagc aacagcgtcg
    27361 tttcgccctg cgtcagggtc ttcatcatgg ccgcgatgct cttggtcttc ggtacatcca
    27421 tgctgatctg gtcgagcaga acgatgtccc ccgcatccgc cttaacagac agagcagagc
    27481 agagcgcggc tcgacgcatc ttgcgcggca tattcttcgt gtatttgcgc ggcagcggac
    27541 catgagcctt gccaccgccg acgaagatcg gcgccttgcg gctgccatgt cgcgcccgac
    27601 cggtaccttt ctgacgatag gccttggcag tcgtgcggtt gacttccgaa cgggtcttgg
    27661 ccttgtgggt gcccaagcgc gcgtttgcca actgacgcac caatgcctga tgcatcaggt
    27721 cacggttaat attcgcttcg aagatagagg ccgggagctc gaaggtgctc acttgttcgc
    27781 cggccatgtt ataaactgga agttccatcg tactgtctcc caagacaact catttccaac
    27841 ttgcggtcgg ttgtcctaac ccttggccgc atcgcggata atgaccaggc cgcccttcgc
    27901 gccgggaacc gcgcccttga tggcgatcag gttccgctcg gaatcaatcc ggacaacttc
    27961 cagattctga gaagtcgaac gttcgttgcc catgcggccg gccattgact tgcccttcgg
    28021 aacgcgtgac atctcggtag ctgagccgat cgagccgggc gcacgccaac ggtcagattg
    28081 gccatgtgtc ttgacgccac cggcataccc atggcgcttc acattgccgg cgaagccgcg
    28141 gccctttgtt ttcccggtca cgtcaacctt ttcaccgacg ataaattgct ccaccgtcag
    28201 ctgctggcca accttgtaat cgccggcgct gctcgtgcgg aactcgcgca ggtgacgtac
    28261 ggccgggatg cctttatcct tgcggcgacc tttcttttca cccttcagaa ttcccagatg
    28321 gccttgctgc cccttggtca accgcttggg tgatacatcg ccatagccca gttgaaccgc
    28381 gctatagcca tcgttctcat tatccttgac ctgggtcaca aagcagggcc cggcctggat
    28441 aatggttaca ggtgtaacga cgcccttatc atcaaagatc tgcgtcatac ccactttctt
    28501 tccaatcaaa cctttcacga gtaacctccc ggcgctcgat aactgtctca gttagagcga
    28561 aatctcgatg tccacaccgg ccggcaggtt cagccgcatc agcgtatcaa tcgtcttcga
    28621 gtcagggttg atcacatcga tcaatcgctt atgagttctg atctcgaaat gttcttgcga
    28681 gtccttgtca atgaacggac tccggcgaac ggtaaaacgc tccagacggg tcggcaacgg
    28741 cacagggccg ataacccgcg cgcctgtgcg ctcggccgtg ccgacaatgc gcttggctga
    28801 ctggtccaga actcgatgat cgtacgcctt caaccggata cggattctct gtttcgacat
    28861 atcacgcctc gttcagcttg gccttacttg ataatcttgg taataacacc ggcgccgacg
    28921 gtcaggccgc cttcgcg
//
LOCUS       DAOMQG010000005        28729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_715_length_28729_cov_11.984237, whole genome shotgun sequence.
ACCESSION   DAOMQG010000005 DAOMQG010000000
VERSION     DAOMQG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 28729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28729
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_28729_cov_11.984237"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..985
                     /locus_tag="PLC06_00735"
     CDS             248..985
                     /locus_tag="PLC06_00735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRO88999.1"
                     /translation="MSTLVPMPPETAANVAAIYARGRELGRDPNAFSKLGDSTILNPH
                     FLGPFDEGAYNLGDFGYLQPTIDRWRGSFKRHGVGAHFGLHSWTVFDPMWADEKWCEP
                     GEHLLACELRLQNPSVLFVRLGSNDSGSPSGFRFNVRDVIKYAIENGVIPIIGTKADR
                     FEGSNENNEILRELAAEYHVPLWDFDLLAGTLPGRGLDKDNIHLVIDDLPHDYTLPET
                     FQRGHPMQDLSALIALDQVRRIIEDTN"
     gene            1072..1482
                     /locus_tag="PLC06_00740"
     CDS             1072..1482
                     /locus_tag="PLC06_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89000.1"
                     /translation="MKQLALLVFSLLLLSLVACGGNSSTNGGANSDPAARAVEKYLSA
                     KVRGDAEAIRAGLCSEMEANLERETRTFESVSNATIEAMSCAGDDPAAASETTVRCSG
                     EIVALYGTEETNFPLGAYRAVIEDGEWKWCGETR"
     gene            1819..2880
                     /locus_tag="PLC06_00745"
     CDS             1819..2880
                     /locus_tag="PLC06_00745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRO89001.1"
                     /translation="MNNQANASIPLLSLKRNRVFGLLFLLILVAGCGQVGSAAPVPTI
                     EEVAALTQPAPLSSPVSTATATLTPTATATALPSSTPTATATATPFPTHTPTPDPTTL
                     VNGVPIEDFIIMPPETQANVRHIFAQGQEMGRNPHAFSKVGDSASLTSHYLVRFDQKR
                     YNLGIYEDLQETIDFYAKSFKRFGVALRIGLHSWIAFRAGEADSDKCGADEHMVQCEF
                     RLHNPSVLLIRLGTNDTASGDAYERAIRFAIEYSIENGVIPVLVTKSDRFEGDNRHNE
                     TLRELAQEYKVPLWDFDVVAGTLPNRGLGTDGVHLTSYDVDDYTDPATLSFGYPLSDL
                     TGLMMLDAIRQTVTQEGNE"
     gene            2877..>4162
                     /locus_tag="PLC06_00750"
     CDS             2877..>4162
                     /locus_tag="PLC06_00750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRO89002.1"
                     /translation="MTLDLTAILVIGLAAVLYAAVIPGRWRAWAVLLGSIVALFWLQA
                     PLAPRYADFLLPSATVALSVAGWWLSRPPRRAFMAPASPATGDPGTAQLDDNNIEVPR
                     VYVRDDWFTLIVVITVILALSLFRYAGADFRLTASRPPSPVFVGITALEVGLVFTALA
                     IILRRLPQRVVLNGAIAAMIALFVVVKWAPATAAVAGWWRGLSGQDITLAAPSDLAWL
                     GFSYVAFRILHTLRDRQTGTLPDLSLRAYLSYILFAPSIVAGPIDRAERFTQDYQALP
                     TLHAFDATRWGMGLWRIAEGLFKKFVIADMLAQGMSLTPAIAAESQNAIALWALLFGY
                     GLRLYFDFGGYSDIAIGLGTLFGIKLPENFIRPYWRTNITAFWQSWHITLSNWARFYV
                     FTPLSRSLLRRRQPDRPSLLTPTTIVLTGHMATMIV"
     assembly_gap    4163..4262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4263..4576
                     /locus_tag="PLC06_00755"
     CDS             <4263..4576
                     /locus_tag="PLC06_00755"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRO89003.1"
                     /translation="LTPTTIVLTGHMATMIVIGLWHGITWNFLIWGTWHALALFAHKQ
                     WSDRTRKWYRQLQTKKWPRRGWATVSWTATILYVMLGWVWFLMPTPEAAIQTFAKLFS
                     F"
     gene            4589..5224
                     /locus_tag="PLC06_00760"
     CDS             4589..5224
                     /locus_tag="PLC06_00760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89004.1"
                     /translation="MINSNSEAQFDQWSATYDHDVQDESGFPFSGYTRVLNRVISLAQ
                     IEPGLEVLELGAGTGNLTGKLVDAGAAVWALDFSADMLAIAKGKVPGAQFGQADLQAE
                     FPPQFRRKYDRVVSTYTFHEVPLSAKIELLHRLVNDYLVQGGRIVIGDIGFINVDARE
                     SVRIASAEAWDDEFYWIRDETMTVLNDKGMALAWEQISSCGAVIEITRRPR"
     gene            complement(5316..6137)
                     /locus_tag="PLC06_00765"
     CDS             complement(5316..6137)
                     /locus_tag="PLC06_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRO89005.1"
                     /translation="MRVIKQYEKGLVIRLGRYDHTADAGLVFLIPFIDSLIRIDMREQ
                     VINVMPQQVITADNVVVTVDAVIYYKVVDPVKAEFEIQNFNFAATTLAQTNLRNLIGD
                     KQLDETLTARDVINTSLREVLDEATNPWGVKVSKVELQKIDPPQDITNAMSKQMKAER
                     EKRANILEAQGLRESAILKAEGEAQARLLRADAEAKSIEMVAQAAEKNFTDRAKDLRR
                     LEVVEKVLAGNSTKFIIPSGSDIVNVLGLDSPDGAHNQVLPVRRSKSIPTTEPSE"
     gene            complement(6211..6660)
                     /locus_tag="PLC06_00770"
     CDS             complement(6211..6660)
                     /locus_tag="PLC06_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRO89006.1"
                     /translation="MDNIVGILPEIFVVIGLLMVLAELVIGIQTGFDLLLIGSIMVIS
                     GIVGILTGSELLMLLLAIGLSVFYIAVGRKKIRQKITSVGKKTNIDKLIGAPGIVVRD
                     INPESAGIIRVDDEQWRAAAEEILYAGDPVVIEAIDGVTLIVRKLTK"
     gene            complement(6924..9551)
                     /locus_tag="PLC06_00775"
     CDS             complement(6924..9551)
                     /locus_tag="PLC06_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRO89007.1"
                     /translation="MRKEARERNIPEFAIAATGAAEDGGIPTIHMAASGDQPPKRTIR
                     GQRRRPGSDRPSSGRADAPTRDDRPSGGGGSYPPTSSGGGMNGIPGGMRGAAGGGGML
                     LALCALIAYMMFGGGGDGQDPFNTGGGIGPSQNQEEFDLGSGLDNPTRPPSQPLPTVN
                     APATREASFAGGNSAITGASGAAASSLPAASTSSATPGQTWTIMLYQDADDKVLEQDI
                     FIDFNEAELVGSSDRVNIVAQMDRYVGGFRGDGDWSDTRRFHLQRDNDLDKINSPYQS
                     IGEVDMSDPQTLYDFATWAIQNYPADKYVLIMSDHGMGWPGGWTDPEPPGSRQINVPI
                     ARAIGDAMYLKDIDAALQAVRDQTGLDAFELVGMDACLMAHMEVFTALAPHARFAAAS
                     QEVEPAVGWAYASFLKDLVDNPDVTGRELGQWIVDSYIVEDQRVVDPNARAQFVGRSG
                     ISAQQLADQLEQGVTLSVADLSEMPAAIEGLNRFSLVLQDADQRAVAKARNYAQSFTS
                     IFGQNVPASYIDLAHFSALLVRETGDANLRTAVEDMFGAFQNLVVAEKSGVKKPAATG
                     LSIYFPNSQLYSSPAAGPASYVPVAERFAGVSLWDDFLNFHYTGRRFQDTAGSLVIPD
                     RNAVIEAPGAGTISVSPLTVDQSTVAPGGAILMSADVDGENLGYSYLFAGYYDREANS
                     INVIDMDFIDSGESREVNGIYYPDWGEGAFTLEFEWEPIVFAINDGTNRVVALLEPQV
                     YGATPELAVYAVDGIYTYQDGTQRRARLLFSNETLLQVISFQEADQNEGSNVMPSPRE
                     ILPSVGDQFTVYERWYDLDENGQMVDVAYEEGGTLTFGESLWTYDVLDAPAGGYAIGY
                     IVEDLDGNRVETYLDVTVE"
     gene            9707..10882
                     /locus_tag="PLC06_00780"
     CDS             9707..10882
                     /locus_tag="PLC06_00780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRO89008.1"
                     /translation="MAIANHSSQGIPPEHTHNDVDWRSIARIIIKAAILFAILNALFA
                     ACRPVEWLGAASLYNRLITGRQRLPYGEVPEKDYNLTLNNIPAMFAAHEWERPKKDDE
                     FRVLLIGDSGTWGWFLRNDDTLAGQLNTMGLKSADGRRVVVYNLGYPVMSLTKDLLLL
                     DEALNRSQADLILWPVTLQSLARGRQLEHPLLHENAERVRALIDRYSLALDKADERFV
                     IRSFIEESIVGRRRDLADLTRLQAWGFAWAATGKDQDIPEDIPLRKSDLEADERWLDI
                     AKPRPLEAGDLSMDVLIAGIRRANTTPIVIINEPIFISEGANSDIRYNSFYPRWAYDE
                     YRELMKALAEAEGWTYLDLWDAIPPEEFTDTPVHLTPNGSRLFAELISAAVFDGSAP"
     gene            10959..11183
                     /locus_tag="PLC06_00785"
     CDS             10959..11183
                     /locus_tag="PLC06_00785"
                     /inference="COORDINATES: protein motif:HMM:NF021552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HRO89009.1"
                     /translation="MCRNIKKLRIPDNPPTDEEIALAALQFVRKVSGYQKPSRVNQPA
                     FEAAVDEIASATRRLLSQLEPKGNPQSDGL"
     gene            complement(11217..13688)
                     /locus_tag="PLC06_00790"
     CDS             complement(11217..13688)
                     /locus_tag="PLC06_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89010.1"
                     /translation="MASRDYLGEQTQPDRLERVVVPEWLRNPAGAIIVAAVIFGLMGV
                     TSLALDVGRPFAKFMTYGYFARSDAEVAHETPRWWEPIATGKVIYGDEVDTINGLPFT
                     PHVREEFDQAYRLGVPVVLTLRRIGEELPITLSFRPDLFTIAEYLDVALPELLVGMVF
                     WLLAVIVLRARPDSTTNQVFAMLAACITIHRLTAITSVITDESLIVNLPKLGHMMAAG
                     MSGPLLIHLALLYPVPLRKMPTKLLAVLYGFGVLSGVILFLTRLPFWRVLPLSVGDFA
                     DAFAYQLMLFLFLVGVVVLFVRLLWLWGNWKKVSLRERRMINIIFVGLIASLPPIIVV
                     LSPLLPFGGETRSTFWQVLDVRYFMLAMPMAFALTIIRYHAFQSPSPLFVTIIVLSLS
                     ALIAAIAAAMWGIVRPDTFSPTERPPFILFFLFIFFPSYFWSRQTDWRGWFGRILNRT
                     DRNYDAVREFGNRVVGRNDIKVLPSAMAHALVEELALERAAVWLWETNSNSYELVGFA
                     GNDTPPADKQIFTLAPLFGRAREVAWPSTPTWIRESALRSNFEAIVPLVAEDRPIGLL
                     GLGRRWDEEIFDERDLAVAELVGQQATLFMTAAIQVDELRRVPARVAQAQERERYRLA
                     SELHDTIQQFLGRLPFFLVVSQELMDTDRDSASKLLDRCMDDVEDAARVLREIRANLA
                     PNQLEVSLIKPLNRLTDHARRRTGLAVYLAAPDDLDDATMAGTRHALYRVIQQALDNI
                     ITHAEATEAEIALGVKDNRVLFSVTDNGKGATEAVLEAARAQGSFGLQSMRARVEAVG
                     GEFSFDSSAGRGTVVSGWVPAPPAP"
     gene            13795..14040
                     /locus_tag="PLC06_00795"
     CDS             13795..14040
                     /locus_tag="PLC06_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRO89011.1"
                     /translation="MDEIIVREKLRAFITRDLVMDDTYPLADGEGIISGGLMDSFALA
                     ELAVFVEREFAVYIPDPDLTVEKMNSLNQIVARVLRG"
     gene            14100..15782
                     /locus_tag="PLC06_00800"
     CDS             14100..15782
                     /locus_tag="PLC06_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRO89012.1"
                     /translation="MPSVKTRGRFNTLLDAITLTAADDDPAIILVSSTNDPVVVTRHA
                     FSRQVQNTANSMSALGIRPRDLVVIAHTQDLESIYSFWGALAIGAIPSMFPALTDKLD
                     PDIYIHNMAELVLLSGVAAVLTTDEFAPHLRNHLSCAVFSSTELANHLTERDFAGAYQ
                     SRPEEIAFLQHSSGTTGLQKGVALSHRAVLNQIAAYSDSIHLAHDDIIVSWLPLYHDM
                     GLIAGFLLPIIQGIPLVLMSPFDWVSHPALLLKAIHDHKGTLCWLPNFAYNHCAHRIR
                     HRDSEGLSIASMRMFINCSEPVHHESHQLFLERFSENGARPEQLAVSYAMAENTFAVT
                     QTSPGVPPRLDVVEGPRLEADRYAKQISKDDPNAQIRVSCGPPIPGTLIRIIAEDGHL
                     PPDRNVGEIAIQSDYLLSSYYKRPDIEPFIDGWFMTGDMGYLADGELFVVGRRKDLII
                     NAGKNIYPADIEAIINQVAGVRPGRAVVFGVANEREGTELVAAVAEVVTEDEEERRAI
                     GRAIRQAVVRQSSVTLSYVHLVGPKWLLKTSSGKIARGANHDKWLREKSGNP"
     gene            complement(15944..16291)
                     /locus_tag="PLC06_00805"
     CDS             complement(15944..16291)
                     /locus_tag="PLC06_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRO89013.1"
                     /translation="MSKKIYVGNLSFQATEDEVREMFAEFGAIESLAMINDRDTGRFR
                     GFAFVEMEDSAANAAIKALNGKDVQGRELTVNEARPREERPSSGGYGNRGGGGGGGNR
                     RGGDYNRGGRNNW"
     gene            16594..18645
                     /locus_tag="PLC06_00810"
     CDS             16594..18645
                     /locus_tag="PLC06_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRO89014.1"
                     /translation="MSENQSTPSKRINLSSLLIGRPLETRTLAHQVISNKVGLAVFAS
                     DAISSTAYATEEILVILALTGTMAHLGLSVPIAIAISILLLIVTISYRQTIFAYPNGG
                     GAYIVARENLGETAALVAGSALLTDYVLTVAVSISSGVAQIVSAFPNLTDFRVPMAVG
                     IIAFVTLVNLRGVRESGTIFAIPTYFFVVMMFLTLAVGFYRWASGSLGTVTGVEFVTE
                     TAAPLTLFLIMRAFSSGSTALTGIEAISNGITAFREPKSKNAARTLTVMSTLLVVLFL
                     GITFLSRHIEAVPSHEETIISQLARTVYGNGSILYLFVIAGTALILLMAANTSYADFP
                     RLAALQAADGFLPRQLTYRGGRLVFSWGIMTLALAASLLVIIMKASTTALIPLYAIGV
                     FLSFTLSQSGMVVHYRKVGQLQPGEIVKGTDSIMEYDSKWRLHMLISGIGATLTFIVM
                     VIFAITKFASGAWVVIIIIPTLVFIFSRIHRHYKDVAQALSLENAHFQVKTDRVVTLL
                     LVDGVHMGTLQMVNFAKSLGNPWKALHVGVNPEKARITQEKWDQYVGDDNLIILQSPY
                     RHLMAPIREYVMNILREQPDAIVHIVMGHLAMDSVLTQALHQNTSLILNLGLTGLERV
                     VVTIVPLQVRQSEEGGDINLNMMTGEDLRRARQAREEAERQQRAERTHRPPPGKKASG
                     L"
     gene            complement(18712..20223)
                     /locus_tag="PLC06_00815"
     CDS             complement(18712..20223)
                     /locus_tag="PLC06_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRO89015.1"
                     /translation="MAEDRNALLNFLARHYTLDELKTLCFKLFIDFDNLSGDTKSAKA
                     RELILHLERSGRIDDLAPVLGREKPVAYRREFQKEPPIPRIPPRRNRDMNQVFISHAH
                     EDEAFARRIAADLQAEGWSVWLAPDSIMPGETWVEAINRGLEECGYYLLVQTPAAAAS
                     PWVVTETNVAISLEHQRQMRFIPLDVAPSRPPPLWTAYQNVPFRDNYLRGLEHLLSRL
                     NGRPPRPWPTNGDAHSRSGNGQGKKGVTESKLFSDRRLHDRTRVEMVRIPAGNFLYGA
                     VQVDPDHEVAQRAIHLHEFWIGCKPVTNAQFARFVEAVQYRTTAEQTGFSRVWSAGRW
                     IEVERAYWRRPEGSRSNIDDRLHHPVVCVSWFDAQAYCEWAGLRLPAEKEWEKAARGT
                     DGRIYPWGNESPTSKRANYAMRHGTTTPGGEFSPAGDSFYGVSDLAGNVWEWTSSWFD
                     SRGSSTQTRVIRGGAWPSEAANLRTTYRLDVDPMLRFNTMGFRVAAHLGDPGF"
     gene            20415..21698
                     /locus_tag="PLC06_00820"
     CDS             20415..21698
                     /locus_tag="PLC06_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="jacalin-like lectin"
                     /protein_id="HRO89016.1"
                     /translation="MDNFHIGPAGGSGGQPFDSYDIPEDARLTAIHVFTEWVINALQF
                     EFIHPDGTPGGNAIIGGLGGEHHVFYLDEDEYLTGISGRAGWYIDSIRFHTSKRVSPT
                     FGGAGGEREFSFDAPEGFELYGLFGRSGWYIDALGVYARRHTEAEGSDESWDEAEDES
                     WLALAGEGEALPASVVVRREVIASNEALDELEDSTLAEAIAEMGADTEGEGTVDAAVY
                     TQIVDDAESGQTVAVIMAVASEVGGVETVGDEPDEVAVMVTDTIESEDDIHLLEEEAV
                     EGAIDALLAEMDEEADEVEVTIYTGVDEREESGKAYAAVVAIATRLEPSADLPSRSAE
                     VAPAGKTRRPPGKGLEIVEGIGPKIAELLMSHDIHDLAELAATPVDKLREILGAAGRR
                     FRLADPATWPEQAALGAAGMWDKLTELQARLKGGK"
     gene            complement(21814..22650)
                     /locus_tag="PLC06_00825"
     CDS             complement(21814..22650)
                     /locus_tag="PLC06_00825"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRO89017.1"
                     /translation="MTNLFTTIIDVEALAGHLADPNWVIVDCRFDLLNKSVGSQHYQA
                     AHIPGAVYAHLEDDLSVPMGPMGEGGRHPLPSPERMAAVFGRLGIGSNTQVIAYDDTS
                     GHYAARLWWMLRFMGHDAVALLDGGWPAWRDAGLPVRSGFEQNEPREFAGSPRWDRLV
                     KMDDVPDAPLLVDGRAPERYRGEVEPIDPIPGHIPGAINRPYSANWTDQGKWRSSEDL
                     LTDYLTLLGEIPPEDAVFYCGSGVSACVNLVGMVRAGIPQGRLYVGSWGEWSRLKPEQ
                     KA"
     gene            complement(22723..23295)
                     /locus_tag="PLC06_00830"
     CDS             complement(22723..23295)
                     /locus_tag="PLC06_00830"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRO89018.1"
                     /translation="MQTRCPWAGNDDEYIAYHDHEWGVPVHDDHKFFEFLILEGAQAG
                     LSWQTILRKRENYRRAFAAFDPVVVAVYDEQKIGELLLDAGLVRNRLKINSAVRNARE
                     FVRIQEEFGSFDSYIWDFVGGRPIVNEWESLRQVPAETPGSRAISKDLKKRGFSFVGP
                     TIIYAFMQATGIVNDHLVSCFRHAQVMAEN"
     gene            complement(23350..>23660)
                     /locus_tag="PLC06_00835"
     CDS             complement(23350..>23660)
                     /locus_tag="PLC06_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89019.1"
                     /translation="RRETESIMDQLEAVENDIAGRLGQLKERAARLHYLESDVNQILQ
                     LASGRDDRMSIETSDRSRQDRVSVLIDHHLRSAHVAMHYEDASVALLRAAEAANKLAL
                     "
     assembly_gap    23661..23760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23761..25403)
                     /locus_tag="PLC06_00840"
     CDS             complement(<23761..25403)
                     /locus_tag="PLC06_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89020.1"
                     /translation="MTWQSLLVPFTLFLIGLVVVLIFIPAYLERLSRVNLAKAAQYQK
                     LQKELEREERRIERLLTPYRRARSDMYRSAVGQVDEQLAHTRGEIAGTAQLMGALQCP
                     QLFDYLFPIQHFAIAPNHVNVIVADARTLQRVKSQLGIIESKLTNASESFDRLADLPA
                     HLHRQQDGLAKRLESLQAVIMRERDEGIEALDDFVRDVAAVGRLMSQAEPVLTDLPEI
                     DAAAFSLQQAEATLAEAELRMTDLERERIALDRRIRRAATELDNAQAFTKSGPGTDAV
                     TQARPILRQAAALLNESAQDHRKRREFNAAGADVTSAVQLITFSRDLLSAHGQIQLLV
                     ERDDGTTLAEPIAALKKEMAELLKRMDGDGVSGYSALSGAAVAGQAARVKTRADTLVR
                     RQDSIIAELQLEAARTKEQLDRSWLAGQNLLSLSPDDPLARRYANLTMKFEGVKPRPT
                     ELEAYRRDAAAFEEIWGKWVARLQASHARIIRIREALPSQIDQSLALAEPWACLTEDL
                     KFIQQRAADFGNLRSRFGDSRYRRETESIMDQLEAVEND"
     gene            complement(25490..25999)
                     /locus_tag="PLC06_00845"
     CDS             complement(25490..25999)
                     /locus_tag="PLC06_00845"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HRO89021.1"
                     /translation="MTQPSLYREVQRFRDVWWVMILVFGLAALQWWIFFMQIVRGIPV
                     GNNPGSDTLVVIIWLVVGIGLPVFFLWLYMVVEVVPGAVIIRYHPFVNRRIPANEIVR
                     VESRTYQPLGEFGGWGVRGWNRRIAYNVHGNSGVELTLTDGRRVLIGSQRADELAGAV
                     YEILGNTGR"
     gene            complement(26001..27329)
                     /locus_tag="PLC06_00850"
     CDS             complement(26001..27329)
                     /locus_tag="PLC06_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO89022.1"
                     /translation="MNEYIDLLRCNPNFRNLWLGSVISQFGDWFNVIAAAELITHLTN
                     SGVALSYLFLARFLPLFVFSPFAGILADRLNRKHIMVFSDVLRGVTVLGFLFINDAGD
                     IWVFYLLTILQFSLSALFTPARGAVLANVVQPSELVAANALDSLTWSTMLAIGALTGG
                     IVAAFFGADAAFIADSFSFFLSAMFIARIVLPAVLAVPLVTGSGGPDSERRFRKRLAF
                     LDGFSYLLNERFILGIALAKAGGSLVWGAINVLEITYANDLFPLQNAARFGIEDPGTA
                     TLGLIYVASGIGTGIGPLVVRHFLGDSPRRLMMGVGLGFATMTIGITWLSTVGRLPTL
                     LMATMIRTLGTGTLWVFTAALLQTIVPDRYRGRVFSFEFAMLTLTQSISVLVGGYLLD
                     TAGMPMQNVMAVVATASAIITLLWLAFHYSYRGPLNEGRIPVRVTSEQGA"
     gene            complement(27490..27606)
                     /gene="rrf"
                     /locus_tag="PLC06_00855"
     rRNA            complement(27490..27606)
                     /gene="rrf"
                     /locus_tag="PLC06_00855"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(27741..>28729)
                     /locus_tag="PLC06_00860"
     rRNA            complement(27741..>28729)
                     /locus_tag="PLC06_00860"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         6680 a         8265 c         7484 g         6100 t
ORIGIN      
        1 caataatacc caggcaacgc caccaaatat tgatacaccc gctactcgcc cggcgcctga
       61 agtggaacag aatgcagggc caactccatc aatagctacg ctcacggccg ttgcatcctt
      121 ggcgggagaa gacggtgaat ctgctaccaa acggccccaa ccagccgtca ctgagacgcc
      181 aacagcgaca ccatcaccgg agccttcgcc tacgccactc ggaccacagg agatcaacgg
      241 catcccattg tcaactcttg tgccgatgcc cccggaaacg gccgccaacg tcgccgctat
      301 ttatgcgcga ggacgggaac tcggccgcga cccgaacgct ttctcaaaat tgggcgacag
      361 tacgatcctg aacccccatt tcctcgggcc attcgacgag ggcgcgtata acctgggtga
      421 ttttggctat ctgcagccga ctatcgatcg gtggcgtggt tcgttcaaac gtcatggggt
      481 aggcgctcat tttggtcttc attcctggac cgtgttcgat cccatgtggg ccgatgaaaa
      541 atggtgcgaa ccaggcgagc acttgctggc ttgcgaattg cggctacaaa atccgtccgt
      601 gcttttcgtg cgactgggca gcaatgactc aggatcaccc agcgggtttc gattcaacgt
      661 cagggacgtg atcaaatatg ccattgaaaa tggcgtcata ccgattatcg gcacgaaggc
      721 cgaccgcttt gagggaagca acgagaacaa cgagattctg cgcgagctgg cggcggaata
      781 ccacgttcca ttgtgggact ttgatttact ggcggggacg ctccccggcc gcgggctgga
      841 caaggataat atccatctcg ttatcgatga tctgccccac gactatacat tgcctgagac
      901 ttttcagcgt gggcatccca tgcaagacct gtccgccctg attgcgctgg atcaggtgcg
      961 caggatcatt gaggatacaa attgatcata gccgcgcgat cattaccctc ctgattacca
     1021 attccactat aattcggatt catccttttt gtggagcatc tgtctgagat tatgaaacaa
     1081 ttggcactac ttgttttctc actgctgttg cttagccttg tggcttgtgg aggcaatagc
     1141 tcaacgaatg gaggggcaaa cagcgatccg gcagcgcgtg ccgtggaaaa gtatctgtcg
     1201 gcgaaggtgc gcggcgacgc agaggcgatt cgcgccggct tgtgctcaga gatggaggcc
     1261 aatcttgaac gagaaacacg cacgttcgag agcgtgagca atgccacgat cgaagctatg
     1321 agctgcgccg gcgatgatcc cgcggccgca agcgaaacga ccgttcggtg cagcggagaa
     1381 atcgtcgccc tctatggcac agaagaaacc aacttccccc tgggggccta ccgcgccgtc
     1441 atcgaggatg gcgaatggaa atggtgcggc gaaacccgat aagtctgcac ggatcggaaa
     1501 ttcagatcgg cgaaatcacg atcagggcct aactccggcc tcgatattca aaagacagag
     1561 agaacctccc ctttcatcga tctccatgcg taaacgaatc ttcaccagag agaacctgtt
     1621 agccttgttc atctgcttgc tggctatttt gctactgatc gtcgcaaccg atagcgcccc
     1681 gcaatggatt tatcaaggct tctaggaggc atgatagccg atcatctgag cgatccttcg
     1741 gcatgggtta ccatggatgc aatcatcggg gaactgaatg cgatcgaata atccgggatg
     1801 gaaaagcgca cacgttgtat gaacaatcaa gccaatgctt caattcccct tctctccttg
     1861 aagcgcaata gggtgttcgg attgcttttc ttgttgatcc tcgtagcagg ttgtggacaa
     1921 gtagggtcgg ccgctcctgt gcctacaatt gaagaagtcg ccgctttgac acagccggcg
     1981 ccactctcaa gcccagtcag cacagccacg gcaaccctga cgcccacggc gacggcgacg
     2041 gcacttccca gctcaacccc cacagcgacg gcaaccgcca cgcctttccc gacccacaca
     2101 ccaactcctg atccgaccac cctcgtaaat ggcgtaccga ttgaagattt catcatcatg
     2161 ccgccggaaa cgcaggcgaa tgttcgtcac atcttcgccc aaggccagga aatgggtcgt
     2221 aacccacacg cattttcaaa ggtcggggat agcgcctccc taacgagtca ttacctcgtt
     2281 cgtttcgacc agaagcgcta caacctgggc atctacgagg atttgcagga aacgattgat
     2341 ttttacgcca aatccttcaa acgatttggc gtcgccttga gaatcggtct ccattcctgg
     2401 attgcttttc gggccgggga ggcagactcg gacaaatgcg gggcagacga gcatatggtc
     2461 caatgcgagt tcaggctcca taaccccagc gtgctattaa ttcgactggg aacgaacgac
     2521 acggcttccg gagatgccta cgagcgagcc attcgcttcg ccattgaata tagcatcgaa
     2581 aacggggtga tccccgtgct ggtgactaaa tccgaccggt tcgaagggga caaccggcat
     2641 aatgagaccc tgcgagaact ggcccaggaa tataaggtgc ccttatggga tttcgatgtc
     2701 gtcgcgggca cgcttcccaa ccgcgggttg ggcacagatg gcgttcattt gacatcgtac
     2761 gatgtggatg attacaccga tccggcgact ctctcgttcg gctatccgct cagcgatctg
     2821 acagggttaa tgatgctgga tgcaattcgc caaactgtga cgcaagaggg gaacgagtga
     2881 cgcttgattt aacggcgatt ctggtgatcg gtctggcggc cgtcctctat gcggccgtta
     2941 tccccggccg ctggcgagca tgggcagtac tgctgggcag cattgtagct ctcttctggc
     3001 ttcaggctcc tctggccccc agatacgccg acttcctatt gcccagcgcg actgtggccc
     3061 tcagcgtcgc cgggtggtgg ttatcgcgcc ctcctcgtcg cgcattcatg gccccggcgt
     3121 cgcctgcaac aggcgatcca ggcacggccc agttagatga caacaatatc gaggtgccga
     3181 gagtctacgt gcgtgacgac tggttcacct tgatcgtcgt catcacagtt attctggctc
     3241 tgtcgttatt tcgctatgca ggcgccgatt ttcgtctcac ggcttcgcgg ccgccctcgc
     3301 cggtttttgt cggcatcaca gctcttgaag taggccttgt tttcaccgcg ctggcgatta
     3361 tcttgcggcg gcttccgcag cgggttgtcc tcaatggggc aatagcggcg atgattgcgt
     3421 tattcgtcgt cgtgaaatgg gctccagcga ctgcggccgt cgccggctgg tggcgcggcc
     3481 tgtccgggca ggatataacg ttagccgcgc catccgatct ggcctggttg ggtttctcct
     3541 acgtcgcctt ccggatcctc cacaccttgc gcgatcggca gaccggcacc ctgcctgacc
     3601 tgtcattgcg cgcataccta agctatatcc tgttcgcacc ctccatcgta gccggcccga
     3661 ttgacagggc cgagcgcttc acgcaggatt atcaggcatt gcccacgctg catgctttcg
     3721 acgcgacgcg atggggcatg ggattgtggc gcatcgcgga aggattgttc aagaaattcg
     3781 tcatcgccga tatgctggca caagggatgt ccttgacccc cgccatcgcc gccgagtcac
     3841 agaatgcaat cgcgctctgg gccctcctgt tcggctacgg cctgcggctc tatttcgatt
     3901 tcggcggcta tagcgacatc gccattggtc tgggtacctt gttcggcatc aagctcccgg
     3961 agaacttcat ccggccctac tggcgcacga acatcaccgc cttctggcaa agttggcaca
     4021 tcacccttag taactgggcc agattctacg tctttacccc gctctcgcgt agcttgctgc
     4081 ggcgacggca gccggaccgg ccatcactcc tcacgccgac aacgattgta ttgaccgggc
     4141 acatggcgac catgatcgtc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nntcctcacg ccgacaacga ttgtattgac cgggcacatg gcgaccatga tcgtcattgg
     4321 gctatggcat ggcattacct ggaactttct gatctggggc acctggcacg cgctggccct
     4381 cttcgcccac aagcaatgga gcgaccgcac gcggaaatgg tatcggcaac tccaaacgaa
     4441 gaaatggccg cgtcgtgggt gggcaactgt gagttggaca gcaacaatcc tttacgtcat
     4501 gctcggctgg gtttggtttc tgatgcctac gcccgaagcc gctatccaaa ctttcgccaa
     4561 attattttcg ttttaacctg atgtctcgat gataaactca aattcagaag cgcaattcga
     4621 tcaatggtca gccacctacg accacgatgt tcaggatgaa tcgggcttcc ccttctcagg
     4681 ctatactcgg gtcttgaatc gggtaatctc attggcccaa atcgaacccg ggctggaagt
     4741 gctggaactc ggagcaggca caggcaacct gaccggtaaa ctggtcgacg ccggcgcggc
     4801 cgtttgggcg ctggactttt cagccgacat gctggctatt gcaaagggaa aagtgcccgg
     4861 agcacagttc gggcaagctg accttcaagc agaattccca ccgcaattca ggcggaaata
     4921 tgatcgcgtc gtgtcaacgt atacctttca tgaggtacca ctctcagcca aaatagaatt
     4981 acttcatcgg ctcgtcaatg actatcttgt acagggcggc cgcatcgtta ttggcgatat
     5041 cggctttatc aatgtcgatg ctcgtgaatc cgttaggatt gcaagcgccg aagcatggga
     5101 cgatgaattt tactggattc gagatgaaac tatgacggta ttgaacgaca agggcatggc
     5161 ccttgcctgg gagcaaatat ccagttgtgg cgcggtgatt gagattaccc gccgaccccg
     5221 ttgataaagg catctctacc atcaatcaaa caaaatgccc caaggatttc aaacccttgg
     5281 ggcattttta gttctcagac ggctgctcaa tcagcttatt cggaaggttc agtcgttgga
     5341 atggatttac tccgccgaac agggagcacc tggttatgcg ccccatcggg agaatccagc
     5401 cccagcacat taacgatatc ggagccggaa gggatgatga acttggtgct gttgccggcc
     5461 aacacttttt caaccacctc aaggcgacgc agatccttgg cccgatcagt gaagtttttc
     5521 tcggcggcct gggcgaccat ttcgatcgat ttggcctcag catccgcccg taacagacgc
     5581 gcctgagcct caccttccgc cttcaggatg gcggattcgc gcaagccttg tgcttccagg
     5641 atattggcac gcttctcacg ctcggctttc atctgcttgc tcatagcgtt ggtaatatcc
     5701 tgcggcggat caatcttctg caattcaacc ttggaaacct tcacacccca agggttcgtc
     5761 gcttcgtcca acacctcgcg caaggaagtg ttgataacgt cacgagcggt cagcgtttca
     5821 tcaagctgtt tgtcgccaat caggttacgc agattcgtct gcgccagggt ggtggccgca
     5881 aaattgaagt tctggatttc gaactccgcc ttaaccgggt cgaccacctt gtaataaatc
     5941 actgcatcga cggtaacaac cacgttatcg gcagtgatga cctgctgggg catcacgttg
     6001 atgacctgct cgcgcatatc aatgcgaatc aggctgtcga taaaggggat cagaaagacc
     6061 agaccggcat cggccgtatg gtcatagcgc ccaagacgaa tgacaaggcc cttctcatac
     6121 tgcttgatga cgcgcacgct gaacgccagc gcgacgacgg caatgataaa aatcagggtt
     6181 aatatgatga ataggaacat gtttctcctg ttacttggtc aacttgcgga cgatcaatgt
     6241 gacgccatcg attgcctcga tcacgacagg atctccggca tacagtattt cttcggccgc
     6301 ggcgcgccat tgctcatcgt caacccggat gatgccggcc gattcaggat tgatatcacg
     6361 cacaacgatt ccgggggcgc ctatcaattt gtcgatattg gtcttcttgc caaccgatgt
     6421 gatcttttgg cgtatcttct ttcgtccgac ggcaatgtag aagacactca aaccaatcgc
     6481 cagcagcagc atcaacagct cactgccggt cagaattcca acaataccgg aaattaccat
     6541 gatcgaaccg atgaggagca gatcaaatcc ggtctggata ccgatgacca gttccgccag
     6601 gaccatcagc agcccgatga cgacgaaaat ttccggcaag attcctacaa tattatccat
     6661 aaatttctat ctcccgaccc ggcacaatcc gctgccggag atatctgttt aagagcaaat
     6721 caatcgaata aaggcaagag gcttgtaacg atctatacgt caatccggat cgtgggttgc
     6781 gccagatatg ccggatgtag aaattataac atatttgccc caaatatggg ggagtccatg
     6841 ttttacacac gtggcagcga acaaacaaaa agggcggcct attctcttcc aggccgccct
     6901 ttttcgatta tgcgagagtc ggtctactcg accgtcacat ccaaataggt ctcaacacgg
     6961 ttaccgtcaa ggtcctcaac gatatagcca atcgcgtagc caccggcagg cgcatcgaga
     7021 acatcgtagg tccatagaga ttcgccgaag gtcagcgtgc ctccttcctc atacgcaacg
     7081 tcgaccatct gtccgttttc gtcaaggtcg taccagcgct cataaaccgt aaattggtcg
     7141 cctactgatg gcaaaatctc gcggggcgac ggcataacat ttgagccctc attctgatcc
     7201 gcttcctgga agctaatgac ctgcagcaag gtttcgttgc tgaatagcag tcgcgctcgt
     7261 cgctgcgtgc cgtcctgata cgtatagatg ccgtccacag catagacggc cagctccgga
     7321 gttgccccat acacttgagg ctccagcaag gcaaccacgc gattggtgcc gtcattgatc
     7381 gcgaagacaa tcggctccca ttcgaactcc agagtgaatg caccctcgcc ccaatcggga
     7441 tagtagatgc cgttgacctc gcgggattca ccactatcga tgaaatccat gtcgataacg
     7501 ttgatggaat tcgcctcgcg gtcgtagtac ccggcaaaga ggtagctgta gcccaggttt
     7561 tcgccatcga cgtcggcgct catcaaaatc gcgccgccgg gagcaaccgt agattgatcg
     7621 accgtcagag gtgaaaccga gattgtcccg gctccgggcg cttcaatcac agcattgcgg
     7681 tcagggatca ccagcgagcc ggccgtatcc tggaatctcc ggcctgtgta atggaagttc
     7741 agaaagtcgt cccacaggga caccccggcg aagcgctcgg ccacaggcac gtaggaagcc
     7801 ggcccggccg cgggcgaact gtagagctgg gagttgggga agtagatcga caaacccgtt
     7861 gccgcgggct tcttgactcc actcttctcg gccacgacga ggttctggaa ggcaccgaac
     7921 atgtcctcaa cggccgtgcg caggttcgca tcgcccgttt cccgcaccag caaagccgag
     7981 aaatgagcca ggtcaatata agacgcgggt acattctgcc cgaatatgct ggtgaaggat
     8041 tgggcgtagt tgcgcgcctt ggcaacagcc ctctggtccg catcctgcaa cacgagcgag
     8101 aatcggttca gcccttcaat cgctgcgggc atttcagaca agtcggcaac cgaaagcgtg
     8161 acgccttgct ccaactggtc ggccaattgt tgcgccgaaa tgccggaccg ccccacaaat
     8221 tgagcgcgcg cattggggtc aacgacgcgc tggtcttcga caatatagct atcgacgatc
     8281 cattgaccca gttcccggcc ggtgacatcg gggttatcga caagatcctt gagaaaagac
     8341 gcataagccc aacccacggc cggctcaacc tcctgtgaag cggcggcaaa gcgcgcatga
     8401 ggagccaggg cggtgaaaac ttccatgtga gccatgaggc aagcatccat gccgacaagc
     8461 tcgaacgcat cgaggccggt ctgatcacgc acggcctgca aggcggcatc gatatctttc
     8521 aggtacatgg cgtcgccgat ggctcgagca atcgggacat tgatctgcct gctccctggt
     8581 ggttcggggt cggtccagcc gccgggccag cccatgccat ggtccgacat aatcaggacg
     8641 tacttatcgg ccgggtaatt ctggatcgcc caggtggcaa agtcatagag cgtctgggga
     8701 tcggacatat cgacttcgcc aatggattgg tatggcgaat tgatcttgtc cagatcatta
     8761 tcgcgctgaa gatggaagcg ccgggtgtcg ctccagtcgc cgtctcctct gaagccgccc
     8821 acatatcgat ccatctgggc gactatattg acacgatccg atgagccgac cagttccgcc
     8881 tcattgaagt cgatgaaaat atcctgctcc aggactttat cgtcagcatc ctgatagagc
     8941 atgatcgtcc aggtttgtcc aggcgtggcg ctgctcgtgg acgcagcggg aagagagctt
     9001 gccgcggcac cggatgcgcc ggtgatcgct gaattcccgc cggcaaacga tgcttccctc
     9061 gtcgccggag cgttgaccgt cggcaatggc tgcgagggcg gccgcgtcgg attgtccagc
     9121 ccgctgccca ggtcaaactc ctcctgattt tgtgaagggc caatgccgcc gccggtattg
     9181 aaggggtcct gcccatcacc gccaccgcca aacatcatgt aggcgataag agcacataga
     9241 gccagcaaca taccaccgcc gccggctgcg ccgcgcatac cgccggggat accgttcatc
     9301 ccaccaccac tggatgttgg aggataactg cccccaccac cggacggccg atcgtcacgc
     9361 gtcggcgcat ccgctcggcc gctgctgggc cgatcgctgc ctggccgacg gcgctggccg
     9421 cggatagtcc gcttgggcgg ctgatcgccg gaagctgcca tatggatggt cggtattccg
     9481 ccatcctcgg ccgcgccggt agcggctatc gcgaactcgg gaatattccg ttctcttgct
     9541 tctttcctca taactttctt tctcctcaat gaggtatcat atatcagagt ttattcttat
     9601 gggtaaaatt acgccatgat tttgtaattt atcgacgcat atcagaggtc gctgtcgaca
     9661 gtataacccc atccacttct ctcattcgca aacatcagga tatcgtgtgg ccatagcaaa
     9721 tcattcatcg cagggaatcc cacccgaaca cacccataat gatgtggatt ggcggagcat
     9781 tgctcgcatt atcatcaaag cggcgatact gttcgcgatc ctcaatgcct tgtttgcggc
     9841 atgccggccc gtggaatggt taggtgcggc ctccctctac aatcggctga ttaccggccg
     9901 tcagaggctt ccctatggcg aagttcctga gaaagattat aacctgaccc tcaataacat
     9961 tccggccatg ttcgcggctc atgaatggga gcggccgaaa aaggacgatg aatttcgcgt
    10021 cttgctcatc ggcgactccg gcacctgggg ctggttccta aggaatgatg acacactggc
    10081 agggcagctg aacacaatgg gtttgaaatc agccgacggc cggcgagtgg ttgtctataa
    10141 tctgggctat ccggtcatgt cattgacgaa agatttgctg ctgttggacg aagctctaaa
    10201 cagatcgcag gccgatctca tcctgtggcc ggtgaccttg caatcccttg cccgtggccg
    10261 gcagctggag caccccctgc tccatgagaa tgccgaacgc gtgcgcgccc taatcgaccg
    10321 ttacagcctc gcgcttgaca aggcagatga gcgattcgtg atacgctcat ttattgaaga
    10381 aagcattgtc gggcgacggc gagacctggc ggatctaacc cggttgcagg cgtggggctt
    10441 tgcctgggcc gctacgggga aggaccagga tatcccggaa gatatccctc ttcgcaaatc
    10501 tgaccttgag gctgatgaac gttggctcga tatcgccaag ccgcgccccc tggaagccgg
    10561 cgatctgtcg atggatgttc tcatcgcggg gatcaggcgg gccaacacga caccgatcgt
    10621 catcatcaac gaacctatct ttatcagcga gggggcaaac agcgatatcc ggtacaactc
    10681 cttctaccca cgttgggcct acgacgagta tcgcgagttg atgaaagcac tggccgaggc
    10741 cgaaggctgg acctatctcg atctttggga cgccataccg ccggaagaat tcaccgatac
    10801 accagtccac cttaccccta atggcagccg attgttcgcc gaattaatct cggcggccgt
    10861 ctttgatggg tctgctccct gaggaatttc gacgagaact tgcttcagtt ggtaaagcga
    10921 acaaaccgtt tttttagcga acgcgctata attcggccat gtgtcgcaat atcaagaaac
    10981 ttcgaatacc cgacaatcca ccgactgatg aagagatcgc tttagccgcg ctccagttcg
    11041 tgcgcaaggt aagcggatat caaaagccat cgcgcgtcaa tcagccggca tttgaagcgg
    11101 ccgttgatga aatcgcctca gcgacgcggc ggctgttgtc tcaactggaa ccaaaaggaa
    11161 acccccaaag cgatggccta taacggagca tcacccgtta tgggcagtca agagggttag
    11221 ggagcgggag gcgcaggtac ccagcccgag acgactgttc cccgcccggc gcttgaatcg
    11281 aaagaaaact caccaccgac agcctccacc cgagcgcgca tcgattgcaa gccgaagctt
    11341 ccttgtgctc gcgctgcctc aagcactgcc tcggtagcgc ccttcccatt gtcggtaacc
    11401 gagaacaaca cccgattatc cttcacccca agggcgatct cggcctcagt cgcttcggca
    11461 tgagtgatga tattatccaa cgcctgctga atcactcgat ataaggcatg acgagtgccg
    11521 gccatcgtcg catcatccag atcatccggt gcggcgaggt atacggccaa gccggtccga
    11581 cgacgcgcgt gatcggtcag cctgttcagc ggcttgataa gacttacctc caactggttg
    11641 ggagcgaggt tggcgcggat ctcgcgcaat acacgcgcag cgtcttcgac gtcatccata
    11701 caacgatcga gcaactttga agcgctatcg cgatcggtat ccataagttc ttgactgacc
    11761 accaggaaga acggcagtcg gccgagaaat tgctggatgg tgtcatgcaa ctcgctggcc
    11821 aaccgatagc gctcacgctc ctgggcctgg gctactcgcg ccggtacacg cctgagttca
    11881 tccacctgaa tcgcggcagt catgaacaag gtggcctgtt gacccacaag ctcggccacg
    11941 gccagatcgc gctcgtcaaa tatctcctca tcccagcggc gccccagacc aagcagccca
    12001 atcggccgat cttcggcgac cagcggcaca atcgcctcga aattgctgcg caatgccgac
    12061 tcccgaatcc aggtcggggt agaaggccag gccacttccc tggcacgacc aaacaatggg
    12121 gcaagcgtga aaatttgttt gtctgccggc ggcgtatcat tgccggcaaa accaacgagt
    12181 tcgtatgaat tcgagttcgt ttcccacaac cagaccgcgg cccgctccaa tgccagctct
    12241 tccaccagag cgtgcgccat tgcactcgga agcaccttga tatcgttgcg tcccaccacg
    12301 cgattgccaa actcccgcac tgcgtcgtaa ttccgatctg tgcgatttaa tattcggcca
    12361 aaccacccac gccagtccgt ctgccgactc caaaagtaac tgggaaagaa aataaagagg
    12421 aagaacaaaa tgaacggcgg gcgctcggta gggctgaacg tatcggggcg aacaattccc
    12481 cacatggccg ccgcgatggc tgcgatcaac gcgctaagcg acagaacaat aatggtgacg
    12541 aacaacggcg aagggctctg aaacgcgtgg taacggatga tagtcagggc gaatgccatc
    12601 ggcatcgcta acataaagta gcgcacatcc agaacctgcc aaaatgtaga gcgtgtttca
    12661 cccccaaatg ggagaagtgg gctaagtaca acgattatcg gcggcaagga agcaatgaga
    12721 ccaacgaata taatattgat catccggcgt tcgcggagag aaaccttctt ccaattgccc
    12781 catagccata aaagacggac gaataaaaca acaacaccca ccaggaacaa gaagagcatt
    12841 agttggtagg caaatgcatc agcaaagtcc ccgacagaaa gaggaagcac gcgccagaag
    12901 ggaagcctcg tgaggaacaa gatgactcca ctaagtaccc caaaaccata caaaactgcc
    12961 aacagctttg tcggcatctt tctcaatgga accggataca acaaagcaag gtggattaaa
    13021 agaggcccgc tcatcccggc agccatcata tgacccagct tgggaagatt tacgatcaga
    13081 ctttcatcgg ttatcaccga agtaatcgcc gtaagccgat ggattgttat acaagcggcc
    13141 aacatagcga atacctgatt cgtggttgaa tccggcctgg ctctgagaac aataactgcc
    13201 aacagccaaa agaccatccc aaccagtaat tcaggcagcg ccacatccag atattcggcg
    13261 atcgtgaata aatccggccg aaagctcagg gttatcggta attcttcccc aatcctgcgg
    13321 agagttaaaa caaccggaac tcccagccgg taggcttggt caaactcctc tcgcacatgc
    13381 ggggtaaaag gtaagccatt gatagtatcg acctcatcgc catagatcac ttttccggtt
    13441 gcgattggct cccaccagcg cggggtctcg tgggccacct ccgcatccga tcgggcaaaa
    13501 tacccatagg tcatgaattt ggcaaagggg cggccgacgt caagcgccag ggaggtgacg
    13561 cccatgagac cgaaaatgac ggccgcgacg atgatcgccc ccgccgggtt acgtaaccac
    13621 tcagggacaa ccacacgctc gagccgatcc ggttgtgttt gttcacccaa gtagtcccgt
    13681 gatgccactg aaaacctccc tgacccacac aaccaggaaa ttgaatggcc acagtataac
    13741 acaaacatcg cccatctttg cgttccggcc tcaactcgat tattctcgct gtccatggat
    13801 gaaataatcg tacgtgagaa gttgcgcgca tttattactc gtgaccttgt tatggatgat
    13861 acctatccgc tagccgacgg cgaaggcatt atcagtggcg gcctcatgga ctctttcgct
    13921 ctggcagaac tggccgtttt tgtggagcga gagtttgccg tctatatccc tgatcccgat
    13981 ctcactgtcg aaaaaatgaa ctcgctcaat cagattgtcg ctcgcgtgct gcgggggtag
    14041 ccgcctgcct cccaaccgcc actaccatca taatctcaag caatacccaa agggtactca
    14101 tgccaagcgt aaaaacacgc ggccgattca acaccctgct tgatgcgatt accctcacgg
    14161 ccgccgacga tgatccggca attattctcg tgagttcgac caatgacccc gtcgttgtca
    14221 cccgccacgc cttcagccgg caagttcaaa atacagcaaa ttccatgtcg gcgctcggca
    14281 taaggccacg cgatcttgtt gtgatcgctc acacccagga ccttgaaagc atttattcat
    14341 tttggggggc actcgccatt ggtgcaatcc cctcaatgtt tccggccctg accgacaagc
    14401 tggacccaga catttatatc cataacatgg ccgaactggt tctgctttcc ggcgtcgcgg
    14461 ccgtgctcac gaccgatgag ttcgcgcctc atttgcgaaa ccatctatca tgtgccgtct
    14521 tcagctctac cgaattggca aatcatttga ccgaaagaga ctttgcaggg gcttatcaaa
    14581 gccggccgga agaaattgcc ttcctgcaac atagcagcgg gacaaccggg ttacagaaag
    14641 gggtcgcgtt gagccacagg gcggtgctca atcaaatcgc cgcatatagc gattccattc
    14701 atcttgcaca tgatgacatc atcgtcagtt ggctgccgct ctaccatgat atggggctaa
    14761 tcgccggctt cttgttgccg atcatccagg gcatccctct ggtcttgatg tcacccttcg
    14821 actgggtgag tcatccggca ctactcctta aggccatcca tgatcacaag gggacattat
    14881 gctggctgcc caatttcgcc tataaccatt gtgctcaccg gattcgccac cgcgatagcg
    14941 aagggctttc catcgcctca atgcgcatgt ttatcaattg ttccgagccg gtgcatcacg
    15001 aaagccacca gctatttctg gagcgattta gcgaaaacgg cgctcgaccc gaacagctgg
    15061 ccgtcagcta cgctatggcc gaaaacacct tcgcggtcac acaaacttct cccggcgttc
    15121 caccccgttt ggacgtagta gaaggccccc ggctcgaagc cgatcgctat gcgaagcaaa
    15181 tatccaaaga cgatcccaat gctcaaattc gtgtttcctg tggccctccg atccccggca
    15241 ccctaattcg gatcatcgcc gaagatggtc atcttccccc ggacagaaac gtgggggaga
    15301 tcgcgattca atccgattat cttctctcaa gctactacaa gcgcccggat atcgagccgt
    15361 ttatcgatgg ctggtttatg acaggggata tgggctatct ggccgatgga gagctattcg
    15421 tcgttggtcg ccgaaaagac ctaatcatta acgccggcaa gaatatttat cccgccgata
    15481 tcgaagccat tattaatcag gtggctgggg tcaggccggg gcgagctgtc gtcttcggcg
    15541 tagcgaatga gcgtgaaggc acggaacttg tggcggccgt cgccgaggtg gtcacagaag
    15601 acgaggaaga gcgacgagcc atcggccgcg caatccgaca agcggtcgtg cgccaaagtt
    15661 cggttacgct cagttacgtc caccttgtcg ggccaaaatg gcttctcaag acatcgagcg
    15721 gcaaaatcgc tcgcggggca aaccatgata aatggttgcg ggaaaaatcc gggaatcctt
    15781 gagcgatttt ctacacgtgc atccacccgg aagtaaacaa cgtaccgcct gatggccgga
    15841 tttgtagccg tctggcagtt tccaacaaaa aagccgtccc gatttgctca ggacggcttt
    15901 ttcatctttt acgattgttt tcgccctttc aggggcaatg aatttaccag ttgttgcggc
    15961 cgccgcgatt gtaatcgccg ccacgacgat tgccaccgcc gccaccaccg ccacgattgc
    16021 cataaccgcc ggatgacgga cgctcttcgc gcgggcgagc ttcgtttaca gtcagctcac
    16081 gaccctggac atccttaccg ttcaaagcct tgatggccgc gttggctgcg gaatcttcca
    16141 tctcgacgaa tgcgaaaccg cggaaacggc ccgtgtcacg atcattaatc atggccagcg
    16201 attcaatagc gccaaactcg gcgaacatct cgcggacctc gtcctcagta gcctggaacg
    16261 acaaattacc tacataaatc ttcttggaca attgaaactc tctcttaaaa ctaaattcgg
    16321 gccaggagca gtgtcacacc actcaggccc ggacgacaac ttcacacagt atacaccaat
    16381 ttatgtcgaa tacaagggct tttaatgtcg ataacctact gcaaggcgct tgcccccaca
    16441 tttcgctcat gttatattca tccgtcggtg ttagccgtca ttatcatgat ctatttgccc
    16501 accctaagga accgcaatta gatatcaggc taaattcagc ccgcggtcga agcatatacc
    16561 aatgtttcgg ctagttcttc acggggtata accatgtcgg aaaatcaatc gacaccatcg
    16621 aaacgaatca atctctccag cctcctgatc ggccggccac tggaaacgcg gacgctggct
    16681 catcaagtta tcagcaacaa agtcggcctg gctgtttttg catcggatgc catctcctcc
    16741 acagcctatg cgactgagga aatcctggtc attctggcat tgaccggaac aatggcccat
    16801 ttggggctat ctgtccccat tgccatcgcc atctccatat tattgctcat cgtcaccatc
    16861 tcctatcggc agacgatatt cgcatatccc aacggcggtg gagcttatat cgtcgctcgg
    16921 gaaaacctcg gagaaacggc cgctctggtg gctggttctg ccctcctgac tgactatgtt
    16981 ctcactgtgg ccgtgtcaat ttcctccggc gtcgcccaga tcgtatcggc attccccaat
    17041 ctgaccgatt tccgggtgcc aatggcagtg ggcatcatcg cttttgtcac actggtaaac
    17101 ctgcgcggtg ttcgggagag tggaaccatc ttcgccattc ctacttattt cttcgtcgtg
    17161 atgatgttcc tcacattggc ggtaggcttc tatcgctggg ccagcgggtc gctcggaacc
    17221 gtcaccggcg tggaattcgt caccgagact gcggcgccgc tcactctctt tctgatcatg
    17281 cgcgcttttt ccagcggttc caccgcattg accggtattg aagcgatatc taacggcatt
    17341 acagcttttc gtgaacccaa gagcaaaaat gcggccagaa cattaacggt catgagcacc
    17401 ctcctggttg tgctatttct cggcatcacc ttcctgtcac gccatatcga ggcagtccca
    17461 tcccacgagg aaacgatcat ctcacagtta gccaggaccg tctatggcaa cggcagcatc
    17521 ttgtatctgt tcgtcatcgc cggcacagca ttgatcttgt tgatggctgc gaacaccagc
    17581 tatgcggact ttccccggct ggcagcgctc caggcggccg acggattcct gccccgccag
    17641 cttacctatc gcggcgggcg gctcgtattt tcgtggggaa tcatgacgct ggctctcgcc
    17701 gcctcgctgc tggttatcat catgaaggcc agcactacgg cattaatccc cctctatgcg
    17761 atcggcgtat tcctgagctt caccctgtct caatcaggta tggtggttca ctaccgcaag
    17821 gtcggtcaat tacagcccgg tgaaatagtt aagggcacag attccattat ggaatacgat
    17881 tcgaaatggc ggctccacat gctcatcagt ggcattggag ccacactaac cttcatcgtt
    17941 atggtgatct tcgccatcac gaagttcgcg agtggggcct gggtcgttat catcatcatc
    18001 ccgacgctcg ttttcatctt ctcgcgcatt catcgccact acaaggacgt cgctcaagcg
    18061 ctaagcctgg aaaacgccca tttccaggtt aaaaccgacc gggtcgtgac attgttgctg
    18121 gtcgatgggg ttcacatggg aacgttacaa atggtcaact ttgccaaatc ccttggcaac
    18181 ccctggaagg cgctgcacgt aggagtgaat ccggaaaaag cccgaatcac tcaggagaaa
    18241 tgggaccagt atgtaggcga cgacaatctg attattcttc agtcccccta tcgccatctc
    18301 atggccccta tccgggaata cgtgatgaat atattgcggg agcagcccga cgccatcgtc
    18361 cacatcgtca tgggtcatct ggctatggat tcggttctga ctcaggcact ccatcagaac
    18421 acctctctca tcctcaattt gggactcact ggcctggagc gcgtggttgt gaccatcgtg
    18481 ccgctccagg ttcgccagag cgaggaaggg ggagacatca acctgaacat gatgaccggc
    18541 gaggacctcc gccgggcacg acaggctcgt gaagaagcag aacggcaaca gcgcgccgaa
    18601 agaacccatc ggccaccccc cggaaagaaa gcgtcaggct tataaaaaaa agaggctccc
    18661 gaattgggag cctcttttta ttgcctaacg acccaatagc gggagcggga attaaaaacc
    18721 gggatcgccc aaatgggccg ctactcgaaa gcccatcgta ttgaagcgca acatgggatc
    18781 gacatccagg cgataggtcg ttcgcaggtt ggcagcctcg ctgggccagg cacccccacg
    18841 aataacgcgc gtctgcgtgg agcttccccg cgaatcgaac caggacgaag tccattccca
    18901 aacattaccc gccagatcgc tgacgccata aaaactatca ccggccggcg agaattcccc
    18961 accgggcgtg gttgtgccgt ggcgcatggc ataatttgca cgcttcgatg taggggattc
    19021 attgccccag gggtaaatcc ggccatccgt cccgcgggcg gccttttccc actccttctc
    19081 ggccggaagg cgaaggccgg cccactcaca ataggcctga gcgtcaaacc agctcacgca
    19141 tacaacagga tggtggagcc gatcatcgat attgctgcga gagccttcgg gacgacgcca
    19201 gtaagcccgc tccacttcaa tccagcggcc ggctgaccag acacgggaaa acccggtctg
    19261 ttcggccgtc gttcgatatt gaacagcttc tacgaatcgg gcaaactggg cgttagtcac
    19321 cggcttgcat ccgatccaga attcatggag atgaatcgct cgctgagcaa cctcatgatc
    19381 aggatcgacc tgcaccgctc catacagaaa gttaccggcc ggtatcctga ccatctcaac
    19441 tcgtgtgcga tcgtgcaagc gtcgatcgga aaacagcttg gactcagtca ctcccttttt
    19501 gccctgacca ttgcctgatc gggaatgggc atcaccattg gtgggccaag gacgcggcgg
    19561 gcgaccattc agccgggata gcaaatgctc cagcccacgc agatagttat cgcgaaaagg
    19621 aacgttctga taggcagtcc aaagcggcgg tggacgactg ggagcaacgt ccagcgggat
    19681 gaagcgcatc tgacgctgat gttccaggct aatagcgaca ttggtctcgg tgacgaccca
    19741 gggcgaggcc gcggccgcag gtgtctgcac cagcagataa tagccacatt cctccaggcc
    19801 cctgttgatg gcttctaccc aggtctcccc cggcataatg gaatcggggg caagccagac
    19861 cgaccaacct tctgcctgca aatcggccgc aatacgccgg gcaaaggctt catcctcatg
    19921 ggcatgactg ataaagacct gattcatgtc acgattgcgt cggggcggaa tgcggggaat
    19981 aggcggttcc ttctggaact cgcggcgata ggcgaccggc ttttcacggc ccagcaccgg
    20041 cgcaagatca tcaatcctgc cgctgcgctc caggtgaaga atgagttctc gagccttggc
    20101 gctcttggtg tcgccgctca gattgtcgaa atcgataaac agcttgaagc aaagcgtctt
    20161 cagctcgtcg agcgtataat gacgcgccag gaagttgagt aatgcgttcc gatcttcagc
    20221 cataatcttg ttgtagcgtt acaacaattg tgccctgcga gcctgctcgc ggcaaccgtt
    20281 tgccaatatt tttacaatgg tcaagtcctc gaagacctgc gcttactacg cttgtcggta
    20341 tggcgcagag gaagaaagta ggccacaata gggcataagg aggagcaatg aatcaatctc
    20401 ttggaggaaa tattatggac aatttccaca ttgggccggc gggcggcagt ggcggccaac
    20461 ctttcgacag ttatgatatc cctgaagatg cgcgattgac ggcgatccac gtattcacgg
    20521 agtgggtcat caatgccctt caatttgagt tcattcaccc ggatggcact cccggtggta
    20581 atgcgataat cggaggcctg ggaggagagc accatgtgtt ctacctcgat gaagatgaat
    20641 atttaaccgg catcagcggc cgtgccggtt ggtacataga ctctattcgc tttcatacca
    20701 gcaaacgagt ttcgcccacc tttggcggtg ccggcgggga gcgcgagttt tccttcgacg
    20761 cccccgaagg atttgagcta tatggattat ttgggcgttc aggatggtat atcgatgcgc
    20821 tgggcgtgta cgcgcggagg cataccgagg ctgaaggaag tgacgagtct tgggacgaag
    20881 ctgaggatga gtcatggttg gcgttggccg gcgagggcga agcgttaccg gcgtccgttg
    20941 tcgttcggcg ggaagtcatt gcttccaatg aggctctgga cgagctggaa gactcaactc
    21001 ttgcagaggc cattgccgag atgggggcgg atacagaggg ggaagggacg gtcgatgcgg
    21061 ccgtctatac acaaatcgtt gacgacgccg agtcgggcca aaccgtcgcg gtcatcatgg
    21121 ctgtcgcctc agaagttggc ggcgtggaaa ccgttggcga tgagccggat gaggtcgcgg
    21181 tcatggtgac ggacacgatc gaatccgaag atgatattca tctgctcgaa gaagaggctg
    21241 ttgaaggcgc aattgacgcg cttctggccg agatggacga ggaagccgat gaggtcgagg
    21301 tgacgatcta taccggcgtc gacgagagag aagagagcgg caaagcctat gccgccgtgg
    21361 tcgccattgc gacgcgcctc gaaccatctg ccgatctgcc gtcacgttcc gcagaggttg
    21421 ccccggccgg caagacgcgc cgccccccgg gaaaaggtct cgaaatagta gaaggcatcg
    21481 gccccaagat cgccgagctg ctcatgtccc atgacatcca tgatctggcc gagcttgcag
    21541 cgacgcccgt ggacaagctc cgcgagattc tcggcgctgc cggccggcgc ttccgactgg
    21601 ccgatcctgc tacctggccg gaacaagccg ctctgggggc cgccggtatg tgggacaaat
    21661 taaccgaact tcaggcgcgg ctcaagggcg gcaaatagac agtcgtcata tcgggcttgc
    21721 ttgaatctac atagcaagaa ggccgatcgc acctatgtgt gatcggcctt ttttaatcgg
    21781 ctcgggctaa cattgggccg caagtaacca gcgctacgct ttctgttccg gctttagtcg
    21841 gctccactca ccccacgaac cgacgtaaag acgcccttgt ggaatcccgg ccctgaccat
    21901 gccaaccagg ttgacacagg ccgacactcc tgagccacaa taaaagacgg cgtcttccgg
    21961 cgggatttct cccagcaagg tcaagtaatc ggttaacagg tcctccgacg agcgccattt
    22021 accctgatcc gtccaattag ctgaataggg acgattgatg gcgccaggga tatgccccgg
    22081 tattggatca atcggctcaa cttcaccgcg atagcgctca ggcgcgcggc catccaccaa
    22141 taggggagca tcgggaacat catccatttt caccagacga tcccatcggg gcgatcctgc
    22201 aaattcgcga ggctcattct gttcgaaccc ggaacggaca ggcaacccgg catcgcgcca
    22261 ggccggccat ccgccgtcga gcaatgccac agcatcatgc cccatgaagc gcagcatcca
    22321 ccacaatcgg gctgcatagt ggccgctggt gtcgtcgtag gcgatcacct gggtattcga
    22381 cccaatgccc aggcggccga atacggcagc catccgttca ggcgaaggta aaggatgccg
    22441 tccgccctcg cccattggcc ccatgggaac cgacagatca tcttccaggt gagcataaac
    22501 cgcgccgggg atatgggcag cctgataatg ctgactaccg accgacttat tgagtagatc
    22561 aaaccggcaa tcgacgatca cccagttcgg atcggcaagg tggccggcga gggcttcaac
    22621 atcaatgatc gtagtaaata ggttagtcat ggaaggcatg atagcacgag agagggcaaa
    22681 tcgcctgtga ttggcacgaa ataatatgac tccacaacat ggtcagttct cagccatgac
    22741 ttgtgcatgt cgaaagcacg atacgagatg atcgttcacg atgccggtag cctgcataaa
    22801 ggcataaatg atcgtcggcc ccacgaagct aaagcctcgt tttttcagat ccttgctgat
    22861 ggctcgggac ccaggcgttt cggccggcac ctggcgcaat gattcccact cgtttacgat
    22921 cggccgccca ccgacaaaat cccaaatata gctatcgaag ctgccgaatt cctcctgaat
    22981 gcggacaaat tcgcgcgcat ttcgaaccgc ggagttgatc ttcaggcgat tgcgaaccaa
    23041 ccccgcgtcc aacagcaatt cgccaatctt ctgttcatcg tagacggcaa caacgacagg
    23101 atcgaatgcg gcaaaggccc gccggtagtt ttcgcgcttc cgcaaaatcg tctgccagct
    23161 caaaccggcc tgggcacctt cgaggatcag aaactcgaaa aacttgtgat catcatgcac
    23221 cggcacaccc cattcatgat catgataggc gatatactcg tcatcattcc cggcccaggg
    23281 gcaacgtgtt tgcattttta agtctttcat atcaacagga tgattccgca cacagggatg
    23341 tttacagggc tagagcgcca gtttattagc cgcttcggcc gcccgcagca aagccacaga
    23401 ggcgtcttcg taatgcatgg ccacatgagc agagcgaaga tggtggtcaa tcagtacgga
    23461 cacccgatcc tgccgggaac gatctgaggt ctcgatcgac attctatcat ctctaccgga
    23521 tgccagctga aggatctgat tgacgtcaga ttccagatag tgcaatcggg cggccctctc
    23581 cttcagctga cccagccgac cggcgatatc gttttccaca gcctcaagct gatccatgat
    23641 cgattccgtt tcacgccggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 atatcgtttt ccacagcctc aagctgatcc atgatcgatt ccgtttcacg ccggtaacgg
    23821 ctatcgccaa aacgagagcg gagattccca aaatcagcgg ctcgctgctg gatgaatttc
    23881 agatcttcgg tcaggcaggc ccacggctca gccagagcca acgattgatc aatttggctc
    23941 ggtaaagcct cacggattcg gataattcgg gcatggctcg cttgcaaccg cgccacccat
    24001 ttgccccaga tctcttcaaa tgcggccgca tcacgcctgt acgcctccaa ctccgttggc
    24061 cttggcttca ctccctcgaa tttcatcgta aggttcgcat atcgccgggc aagcggatca
    24121 tcaggagata gactgagcaa attctggcct gccaaccagg atcgatccag ttgctccttc
    24181 gttcgcgcgg cttccaattg caattcggct attatcgaat cctggcggcg aacaagcgta
    24241 tcggctcgcg tcttgactcg cgcggcttgg ccggcaacgg ctgcgccgga aagggcacta
    24301 tagccgctca cgccgtcgcc atccatacgc ttaagcagct cggccatttc cttcttcaga
    24361 gcggcgatag gctcagccaa ggttgtgccg tcatcacgtt ccacaagtaa ctgtatttgc
    24421 ccgtgggccg acagcaaatc gcgactaaaa gtgatgagtt gaacggcgct cgttacgtct
    24481 gcgccggcag cgttgaattc acggcgtttc ctgtgatcct gagcactctc gttgagcagc
    24541 gccgctgcct gtcgcaatat cggccgagcc tgcgtcactg catccgtgcc gggaccggat
    24601 ttcgtgaatg cctgggcgtt atccaactca gtggcggcgc gccggatgcg gcggtcgagg
    24661 gcgatccgct cgcgttccag atccgtcatc cgcaactcgg cttcagcaag cgtggcctca
    24721 gcctgctgaa gagaaaaagc ggccgcgtct atctcgggca agtcagttaa aacaggctca
    24781 gcctggctca tcaggcggcc aactgcggca acatcacgca cgaagtcatc cagcgcctca
    24841 atgccttcat ctcgctcgcg cataattaca gcctggagcg attccagccg tttagccagg
    24901 ccatcctgtt ggcgatgcaa atgggccggc aaatcggcga ggcgatcaaa tgactcgctc
    24961 gcgttggtta atttgctttc gataatccct agttgggatt ttacacgctg caaagtccgt
    25021 gcatcagcca cgatgacatt gacgtggttg ggagctatcg caaagtgctg gatgggaaag
    25081 agataatcaa acagttgagg acactgtaat gctcccatca attgtgcggt tccggcaatc
    25141 tcgcctctgg tatgtgcgag ttgctcatcg acctgaccga cggccgaacg gtacatatca
    25201 gacctggcgc gccgatatgg cgttagcaat cgttcaatcc ggcgctcttc gcgctccagt
    25261 tccttctgta acttctgata ctgtgctgct ttggccagat tgacgcgcga caaacgctca
    25321 agataggccg gtatgaatat gagcactaca accagaccta tcagaaataa cgtaaaagga
    25381 acaagaaggg attgccaggt cattccatcc acccaaggta tatcccatta tataagcaaa
    25441 agcccgtcag gcgatgatga ccggtcagac ttgccatcaa ttgccggggt catcgcccag
    25501 tgttgcccag tatttcgtaa actgcaccgg ctaattcatc cgcccgttga ctgccaatga
    25561 gaacacggcg gccatctgtc aacgtaagct cgaccccgga attgccgtgc acattatagg
    25621 ctatacgcct attccagccg cgaactcccc aaccgccaaa ctcccccaaa ggctgatagg
    25681 ttcgtgactc aacacgaacg atctcattgg ccggtatccg ccgattgacg aaaggatgat
    25741 agcggatgat aaccgctccg ggaacgacct ccacaaccat ataaagccac aagaaaaata
    25801 cggggagccc gatgccgacg accaaccaga tgatcacaac cagtgtgtcc gatcccggat
    25861 tattgcccac tggaatgccg cgaacgatct gcatgaaaaa gatccaccac tggagagcgg
    25921 ccagcccaaa gaccaatatc atcacccacc agacgtcacg aaaacgttgg acttcacgat
    25981 aaagagatgg ttgcgtcata tcatgcgcct tgctcgctgg ttacacgtac aggaatgcgc
    26041 ccttcattca agggccctcg gtatgaataa tgaaacgcca accacagcag cgttataatg
    26101 gccgacgctg tagccacaac agccatgaca ttctgcatcg gcataccggc cgtatccaac
    26161 agatatcctc cgacaaggac tgaaatcgat tgggtcagtg ttagcatggc gaactcaaat
    26221 gaaaacacac gtccacggta cctgtcggga acgatcgtct gcaacagagc ggctgtgaaa
    26281 acccacaagg tgccggttcc caaggttcga atcatcgtcg ccataagcaa ggttggtaat
    26341 cgaccaactg tggataacca ggtgataccg atggtcatgg tggcgaagcc aaggccaacc
    26401 cccatcatca gacgccgggg ggaatcgccc agaaaatgac ggacaaccag cggccctatc
    26461 cccgtgccga tgccgctggc gacgtagatg aggcccagag tcgccgtccc aggatcctcg
    26521 atcccgaatc gagccgcgtt ctgcaagggg aaaaggtcgt ttgcataggt gatctccagc
    26581 acgttaatcg ctccccatac cagtgaacca cctgccttgg ccaaagcgat acccaaaata
    26641 aaccgctcgt ttaacaaata gctgaatcca tccagaaacg ccagccgttt acgaaagcgc
    26701 ctctcggaat ctggcccacc gctgcctgta accaacggca cagcaagcac ggccggcaat
    26761 acgatgcggg cgataaacat cgctgaaagg aaaaatgaga atgaatcggc gataaaagct
    26821 gcgtctgcgc cgaaaaaggc cgcaacaatc cctcctgtca atgcaccaat ggccagcatg
    26881 gtgctccagg tcaggctatc aagcgcattg gcagcgacca actcgctcgg ctgcacgacg
    26941 ttcgccagca ccgccccgcg ggcaggagta aacaacgcac tgaggctgaa ttggaggatg
    27001 gtcaatagat agaaaaccca tatgtcaccg gcatcattga tgaacagaaa accaagcacc
    27061 gtcacacccc gcaggacatc ggagaaaacc atgatgtgct tgcgattcag gcggtcggcc
    27121 agaatcccgg caaaagggct aaacacgaaa aggggcagaa accgtgccag gaacaggtag
    27181 ctgagggcaa cgccactgtt agtcagatgg gtaatcagct ccgcggcggc tatgacgttg
    27241 aaccagtcgc caaattgaga aataacgctt cccaaccaga gattgcgaaa gttggggtta
    27301 caacggagca gatcgatgta ttcgttcatt cgcttgtaat tctgactcaa agccgacgcg
    27361 ggggcaatcc catgaggctg agggcatgga ccaaatctat agacacaaac aaaaaagggc
    27421 ggtcaaccct tgtcggtcag ccgccctcaa ataaattctg tgctatccaa atataaaaca
    27481 aaaaaggcct tctggcaatg acctactctc ccaggaagtt gccctccaag taccatcggc
    27541 gctgtcgagc ttaactgccg ggttcgggat gggaccgggt gtgacctcga cgctcaaatc
    27601 accagaagac ccttttaatt aactttcgta agggcaggtt tgaagcctgc ccctacaaag
    27661 gttgggtcaa cttgaataga tgaagacacg gggtccgtgc atgattacga accaaaatcc
    27721 ccaattctcc gcaccatatg catagtaagc cctcgaccat tagtacggct tggctgaaca
    27781 tattgctatg cgtacacctg ccgcctatca aactagtagt cttctagtgg tcttactcca
    27841 tacggatgag ggatctagtc ttgagacgag tttcccactt agatgctttc agcggttatc
    27901 tctgccagac gtagctaccc agcaatgccc ctggcggaac aactggcaca ctagaggtct
    27961 gtccatcccg gtcctctcgt actagggaca gcttctctca aatcccttac gcccacagcg
    28021 gatagagacc gacctgtctc acgacggtct gaacccagct cacgtaccac tttaatgggc
    28081 gaacagccca acccttggga ccttatccag ccccaggatg tgatgagccg acatcgaggt
    28141 ggcgaacctg gtcgtcgata tgaactcttg gaccagacta ccctgttatc cccggagtag
    28201 cttttatccg gtaagccacg gccattccac tctgaaccgt gggatcacta agccctactt
    28261 tcgtacctgc gcgactcgtt ggtcttgcag tcaagctccc ttgtgccttt acactcaacg
    28321 gctggtttcc attcagcctg agggaacctt tgggcgcctc tgttactctt tcggaggcga
    28381 ccgccccagt caaactaccc accagccact gtccccatgc cggataacgg ctacaggtta
    28441 gagccaagaa taaaccaggg tggtatttca acggtggctc caccgagact ggcgtcccag
    28501 cttcaaagcc tcccacctat gctacacagg tttagcccta actcaatggc aagttatagt
    28561 aaagcttcac ggggtctttt tgtcccgctg cgggtcacgt gtatcttcac acgtccttca
    28621 atgttcgccg agtccctcgt tgagacagtg ctcctctcat tacgccattc gtgcgggtcg
    28681 gaacttaccc gacaaggaat ttcgctacct taggaccgtt atagttacg
//
LOCUS       DAOMQG010000006        28618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_718_length_28618_cov_11.214998, whole genome shotgun sequence.
ACCESSION   DAOMQG010000006 DAOMQG010000000
VERSION     DAOMQG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 28618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28618
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_28618_cov_11.214998"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLC06_00865"
     CDS             complement(<1..181)
                     /locus_tag="PLC06_00865"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HRO89023.1"
                     /translation="MTTNGRLYLSAPVNAIVEGIYEEHVPFSEIRRHGDFGLGTFDLL
                     DGEMVMLDGRIYQMTA"
     gene            complement(187..483)
                     /locus_tag="PLC06_00870"
     CDS             complement(187..483)
                     /locus_tag="PLC06_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89024.1"
                     /translation="MFAVVNHLYFNKPVDEIRDSIMNEGMPILVGLPGFRDFYLVKSA
                     EDRAIVILLWEDALSAINGSKVFGPTWFNDNIIPHLAGEQQRSVGPVIVASEPK"
     gene            complement(571..>2025)
                     /locus_tag="PLC06_00875"
     CDS             complement(571..>2025)
                     /locus_tag="PLC06_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRO89025.1"
                     /translation="TFMTGVNPGKHGVFDFIQPHGENFTLVNSTQIRQPTIFQRLSQA
                     GYRVGVLNVPVTYPPQEVNGFMVTDILSPKNATISYPADLISRYEPELGPYRVAPNIQ
                     YKAGNEAEFIADIYDLIDAHTRWALKLLESEPVDVLMVHFISMDIAKHALWRFMDHDH
                     ERYEPSPWEHAIRDGYAKVDASIGQLMARLPAGSTTIAMSDHGFGPLKNMVNLNVFFM
                     QRGLMKLKPDAVTQLKAWAFSHGITPAGAYQWIARAGLQNTVAQVSKKARNDIVGKFL
                     SFDSVDWSRTIAYSMGHVGQVYINNIGRDGHGIVAEADYERRLTEVSDALTELRDDDG
                     RPILTNLWRGREVYHGPYAHLGPDLHLELDHYAMIACPLFATEGRVMTNQIRGDSGCH
                     RREGIFIAAGPAIHNGVELPEVNILDLAPTIMHLLGEPVPRVMDGRVLDEIFTQSNPV
                     QFSEDDDANMTAQQGFDAAEAEQIEERLRGLGYL"
     assembly_gap    2026..2125
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2126..2339)
                     /locus_tag="PLC06_00880"
     CDS             complement(<2126..2339)
                     /locus_tag="PLC06_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRO89026.1"
                     /translation="MTLLIIGFDGATFDLIKPWARDGFLPNLAGLMRDGVTADLASTL
                     PPVTSPAWPTFMTGVNPGKHGVFDFIQ"
     gene            complement(2336..2611)
                     /locus_tag="PLC06_00885"
     CDS             complement(2336..2611)
                     /locus_tag="PLC06_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRO89027.1"
                     /translation="MFPRITHVQHVRDYILHLKFTNGEEADIDLESRIRNRGGVFSPL
                     EKIEYFARVAVDPEAHTLVWPNGVDLDPDVLYSVATGTPLPALQAIQ"
     gene            complement(2628..2888)
                     /locus_tag="PLC06_00890"
     CDS             complement(2628..2888)
                     /locus_tag="PLC06_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRO89028.1"
                     /translation="MPELSRFFGIIIRMYYDDHPPPHFHAIYGQYEIQVSIDPIVILN
                     GQLPRRAISMVIEWAAIHQQELMDNWKRLEVNQTMVRIEPLD"
     gene            complement(2907..3920)
                     /locus_tag="PLC06_00895"
     CDS             complement(2907..3920)
                     /locus_tag="PLC06_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89029.1"
                     /translation="MTPITTKGLSLVIPVYNEEENLRPLAEEIRVALDGTGLDYEAIF
                     IDDGSKDRSFTVLAELAAEDDRIVAIRFRRNYGQTAAFAAGFDHARGRVIVTLDADRQ
                     NDPADIPAMLAELDKGYDVVNGWRENRQDDLVRRFPSRVANWLIARTSGVHLKDRGCS
                     LRAFRSEVVRDLHLYGELHRFIPELVNFAGFTMSEVPVNHRARVAGTSKYGLSRTFRV
                     ILDLFTIHFLRKYGDRPMQLFGRWGIILFALGALIGGYLTALKLWAGITGGIEGFNAM
                     TIGDRPLLLLSVLLVIIGVQFIIMGLIAELTVRTYYETQDLRVYRVREIIGQESSTFN
                     QLK"
     gene            complement(3924..4292)
                     /locus_tag="PLC06_00900"
     CDS             complement(3924..4292)
                     /locus_tag="PLC06_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRO89030.1"
                     /translation="MEVKPIRNEADYQCALKEIDRLLNAPPDATDEDRLEVLITLVDA
                     YEEATDPIDAPDPIEAIHHYLESQELSFDELEPYLGRGQVKAVLERRIPLSLEMIRNL
                     REGLGIPAEILIQRYELEVA"
     gene            complement(4369..4662)
                     /locus_tag="PLC06_00905"
     CDS             complement(4369..4662)
                     /locus_tag="PLC06_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRO89031.1"
                     /translation="MRIIARRTLREFWEKHPDAEQPLRTWYTRVRNADWQTPADVKQA
                     YRTASFIGNDRVVFNIKGNGYRLVVVVVYRHHAVYVRFIGTHREYDDIDVSTV"
     gene            complement(4668..6332)
                     /locus_tag="PLC06_00910"
     CDS             complement(4668..6332)
                     /locus_tag="PLC06_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRO89032.1"
                     /translation="MTQRKVAVFGLDGITFDLLQPWLDEGHLPNLARLLAEGAGGRLR
                     STIPPVSASAWPSFATGTNPGQHSLIDFTYPAQDSYEIRVSNGLTVAVPAVWQIAGAA
                     GKQVGVVSLPMTYPPRPMNGFMLCSFLTPSADSDYTWPPELKQELIDACGPFPLRMSE
                     KGRGVDPAVFVRACKQLEIDRARNVQYLLREKPWDLFAYVMETTDNLQHEVWHLVDKS
                     HPMHDPEMAIRVLPDILDYYETVDRLLGEMVALVPEEALVIVLSDHGFGPFHKFFHIN
                     NWLADNGWLVFKRSPMSRLKRAAFKLGVTPINALKWLTALGLSDMRKNVKRGRGGKTL
                     RRFFLSFNDVDWSRTKAFSVGNFGQVYLNVKGQRPQGAVDPAEYESLRDEIIARAKEL
                     RDPKDNSQVVPVVYRREEVFHGISAARLPDLVLHTDRAKYVSFGHADFGSNRIIEPST
                     GQTGHHHMEGVVGLRGPDVRPGVKLEGASLLDPAPTILHYLGLPVPEYMDGHVLTDAF
                     TDVFNEANPITYSQIGPGDGVGSDAIYTDAEEELVMQKLRDLGYVA"
     gene            complement(6376..7377)
                     /locus_tag="PLC06_00915"
     CDS             complement(6376..7377)
                     /locus_tag="PLC06_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRO89033.1"
                     /translation="MGKFLKANRKKLFGLSQILLSIILLVWLLRQVGLTEVMEALANI
                     HWGWYALAFLLFQLNAVIRAYRWYVLLRALNDKPSFGYLIYLYYIGFFANNFIPSGFG
                     GDLVKVVSLRQSHGHGAEALSSVLMERITGLVGSSLVALIALTWNLAVHATTLDLPWI
                     LWALIVVTAVGIPLAFFIARWANPIGLLNRVYPNAGNLPFYNKIENLVNTIHRYPLRA
                     LISALLISLPFTLNLILVQMTVALALGVHLPFRIFALFVPVIAIINLLPITFNGLGLR
                     EGIYTFLFVPVGIPAATAVSMSLAFYFLRFSAGLLGGLMYAFRSILSFARPPEAHKL"
     gene            complement(7534..7914)
                     /locus_tag="PLC06_00920"
     CDS             complement(7534..7914)
                     /locus_tag="PLC06_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89034.1"
                     /translation="MDKVVTDRLIRPIVVIFFAGFLLILAGLCIFVIVRGSVMWPVPV
                     IVAVICVIMAQGIWRLKEWARLFTIFLLGVSLFIVPLAVAGYWWAQRIVPIADLLAII
                     CACPPLVPLGLLLYWFLSNEEHFQ"
     gene            complement(7907..8356)
                     /locus_tag="PLC06_00925"
     CDS             complement(7907..8356)
                     /locus_tag="PLC06_00925"
                     /inference="COORDINATES: protein motif:HMM:NF039927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HRO89035.1"
                     /translation="MIDQCPECGAPQAINQTCRDHFHQMLFWENENPTRWEVHHLMVL
                     CYHLQHPGLYSAEGLAVARGLLADFIEHGLSPEEVRKKNSGRVASGARDWSITARPGN
                     QGAYDRPIRWTMTAADVVAGGPENYVVNVRTWASEVHRTLTEGGHYG"
     gene            8455..9534
                     /locus_tag="PLC06_00930"
     CDS             8455..9534
                     /locus_tag="PLC06_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRO89036.1"
                     /translation="MRAIRVHEYGGPDVLHLEEIDMPEPGWGEARVMLKAAGVNFVDI
                     YQRSGQYKMNLPYTPGREGGGIVDAVGPNVTDVAPGDYVVFAMHLGAYADYAIVPAWM
                     LAPVPPGIDMMSATAAVLQGMTAHYLTHDTYPIKPGDTVLIHAAAGGTGLLLVQMAKM
                     RGARVIGTASTEEKEQLARQAGADEIVRYAEQDFEAEVKRLTGGRGVDAVYDSVGRDT
                     FLKSLNCLRPHGYLVLFGQSSGAVAPFDPQMLNHAGSVYLIRPSLHHYIRDRKEILRR
                     TSDVYTWLGDGRLTVRIDKTFPLEDAADAHRYLEGRQSKGKVLLGTQMGRELKVGLLN
                     RAIERGDVIDEAGWESFPASDPPAY"
     gene            complement(9568..9798)
                     /locus_tag="PLC06_00935"
     CDS             complement(9568..9798)
                     /locus_tag="PLC06_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89037.1"
                     /translation="MIQVISVIGALLILIPFAANQFERLTRDSFVYQLMNLAGSGTLA
                     AVALVERQYGFLLLEGTWALVSLWGLASVLKK"
     gene            complement(9852..11291)
                     /locus_tag="PLC06_00940"
     CDS             complement(9852..11291)
                     /locus_tag="PLC06_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89038.1"
                     /translation="MFRTLRGRLLLSFVVVILVGLLIVAVSLFGFASVSDARLLPSLE
                     RLSAISRTNQRELLQLWGAGAGSSELQGLLHNTSEQADVRILVVDTSAEEIVFDTAVG
                     DDWVGDRLTNVDRPQSLVLTNVRRGSIFGTFVHTDGSRWLVFAEPDPALGLALIFYAQ
                     PEPTAGEFFNEFFLRPLFNAGVLAVLLAMLLAAIIAQSVARPLGAIAVAAEAVARGDY
                     DQHVPQEGPDEVRRVAGSFNSMAAQVKTTQQAQRDFVANVSHDLKTPLTAISGWSQAL
                     IDGAAEGPEERKRAAETIHAEADRMSRLVNELLDLARLESGQMPLTMQRVDLNDILAG
                     VHRSQLPNARAREIDLALGVAGEAFVMGDPDRLAQIFTNLVDNALAYTPAGGIVRLTG
                     RLNGNQVEGIVSDTGQGIPEEEIPRVFERFYRLEKSRARGEHGRGSGLGLAIVHELVT
                     AHGGTIGVSSQVGRGTVFVVRFPAPSSRQ"
     gene            complement(11393..13951)
                     /gene="phnE"
                     /locus_tag="PLC06_00945"
     CDS             complement(11393..13951)
                     /gene="phnE"
                     /locus_tag="PLC06_00945"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRO89039.1"
                     /translation="MPSENKTSLSPWLPAFLSLIIPGSGQIILSHKTRGFALFLAFFA
                     LLGLVLWTQAYALLAPLALLLIWIARDAYRLAKGSEPSWGVSLLLIGIVLYGTALIVT
                     EVRPTRMITGLPNVTPYLRSLFNPELFETPMKEVVGVTPIMVPCVDPLPAPNREATTS
                     PQLILSAPCTEVGDLLQVTGAGFEPNESGQMQWIDPLGSPRRATFDGEVVTFKADENG
                     RFDVTLLVPQAVPLTAQPAPGETLTHAVRAVQNIPSGRLQPTQTLSLVIEKIGETIAL
                     AFLATVMGVIFAVPVSFLAARNLMSGNPVTMLIYNVVRAILNVIRSIETLLWAIIFAV
                     WVGLGPFAGTLALWFHTVAALAKLYSEAIESIDSGPIEAVKATGASWPQMVIYAVFPQ
                     ILPTFTSFTLYRFDINVRLSTVIGLVSDAGLGFLVVQWVRLNRFSAMATALIAIILVV
                     AILDFLSSWLRERIIQGRPIISSTNPLVRTVLKTVIIVGFVATFIWSWNVAQIKLIEL
                     VKGAPQGLALAREFATPELFTRPTKTVAISAPLTVPCGAAEPSTPADATITLSADCGE
                     TGDPLVIEGTGLPPNRTVSVRWVLPDGGYLRVRSNCCDTDDDGNLRVETAINPIVVME
                     EGQTEPARVEITYEEIAGRIQLSETVRTVIRLSIVTLLMALVATTLGALFAIPLSFLA
                     ARNIMGDTPAGRTIYYGLRTFFNVARSIEPLILVLIAATWVGAGPFAGVLALALNNIP
                     NLGKLFSDSIEEINSGPVEAVTSTGATRMQSLVYAVVPQLVPPFLAFIIYQWDINIRM
                     STVIGFVGGGGIGQQFRIWVSLNQYGAAGTAILAIVIMVWTMDYLSAKARERLI"
     gene            complement(13941..14714)
                     /gene="phnC"
                     /locus_tag="PLC06_00950"
     CDS             complement(13941..14714)
                     /gene="phnC"
                     /locus_tag="PLC06_00950"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRO89040.1"
                     /translation="MLQIKNLTKIFPNGTVALNNVSFDVQDGEFLVIIGLSGSGKSTL
                     LRCINRLIEPTSGAVLWNGQNITEAGSAELKQIRRQIGMIFQHFNLVKRSSVTTNVLT
                     GRLGYVHPVWSLFGRFTADDRRRALAALDRVGIADKADSRADQLSGGQQQRVGIARAL
                     MQEPQLLLADEPVASLDPVLSHSILRYLELLNQQDSITVLCSLHFLDLVHRYATRVVG
                     LKGGELVFDGLPSELTPEKFKEVYGEEAEMVSVAPPNAV"
     gene            complement(14797..15696)
                     /locus_tag="PLC06_00955"
     CDS             complement(14797..15696)
                     /locus_tag="PLC06_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRO89041.1"
                     /translation="MNARKLLLLVSMVAVLVFALAACGGAPKLGTAENPIIMSFVPSG
                     DTQDIIASGDTLAQMVSERTGLVVKANVGTDFSSVREAMCAGKAQIGWLNTFNYVLAN
                     EQCGVDAALATSRFGSSTYAGQIIVRADSGISTIADLKGKVMCWVDPASTSGYIIPRI
                     MLAAEGVNPDTDFSQTVEAGSHNNVVTQVYNGDCDAGATFSDARTGIEKEFPDVLEKV
                     VVLATTSDIPNDSVSFIKDFPAEMRTKIVAALLDISASPEGQEALNTLYNIESLVESS
                     DSFYDVFRADLSRAGIDIESLAK"
     gene            complement(15873..16496)
                     /locus_tag="PLC06_00960"
     CDS             complement(15873..16496)
                     /locus_tag="PLC06_00960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRO89042.1"
                     /translation="MTKISLIRHGLVHNPAEVYYGRLPGYGLAALGRAQAAAAGDYLA
                     GAGIVAVYHSPMLRAFQTAEIVRSRCAANAPLVECDLLNEIHSPFDGLAAAEMEKRDW
                     NFYDEINPPYEQPADIVDRLLRFFDLARQRHPGRHVVGVSHADPIAFAILWAADLSLS
                     ASQRKRLIDAGISEPYPSPASVTTFEFADSEERKLLTFRYHAPAALL"
     gene            complement(16510..17193)
                     /locus_tag="PLC06_00965"
     CDS             complement(16510..17193)
                     /locus_tag="PLC06_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89043.1"
                     /translation="MPATILIADDEANIRDLVRLYLEKEGFRVQTAENGRQALEQAQS
                     GSPDLIVLDLMMPELDGWEVCRRLRAEGSLPILMLTARDQDIDKIVALEMGADDYLTK
                     PFNPRELVARVRAILRRTSGGRPGPDQPRRLGRLTLNPASREVTVGDEEIALRAREFD
                     LLLALIDHANQVLSRDQLLDLVWGYEYHGQTRTVDVHVAQLRDRLTNSDVEIETVWGR
                     GYKLNVRAA"
     gene            complement(17294..17677)
                     /locus_tag="PLC06_00970"
     CDS             complement(17294..17677)
                     /locus_tag="PLC06_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRO89044.1"
                     /translation="MEKKRVHTNDAPAAVGPYSQAIRIGDFVFTAGQVALDPASGEMV
                     GNDVAAQTEQVMSNLGAVLAAAGSDLSRVIKTTVFLQSMGDFAAMNAVYARYFPEPFP
                     ARSTVEVGALPKGGLVEIECIAHAQ"
     gene            complement(17696..18244)
                     /locus_tag="PLC06_00975"
     CDS             complement(17696..18244)
                     /locus_tag="PLC06_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89045.1"
                     /translation="MAPGQDDQVIQCPNCRSAVAIADEGLNILPAQYGSPAVEAPPRW
                     LPFWVCRGRVTILKRKTQAGQSSERVAREFWAEPRRVYIPAWACDLLEARDMVESLLE
                     QQPILEAGTPPEGVIFDPAVVAPADARKLLELVVLTIEAERSDYLEAFEFELSLEDEA
                     LWLFPAERQKDRWKILLKYISP"
     gene            complement(18291..19322)
                     /locus_tag="PLC06_00980"
     CDS             complement(18291..19322)
                     /locus_tag="PLC06_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89046.1"
                     /translation="MPVAEGVRIVQCPYCSLHSLVQGDRGIRRWQVARRIDRATAEQS
                     VRGFLGGMRKASNLRRTAKIDEMLVAYLPFWRVEATVAGWLFGRVRKDKDETKPDEHA
                     VFESMHWNDAAVDVSEFGVHRIVVSRDDLQPFDSQALHAEAMVFEPSESRTAALDEAQ
                     AHFTYRSQVAAGQSKTHYENIQLLRPEFSLVYFPVWLARYSYRNRVYQVVVDATSGKV
                     MYGKAPGNTLYRAAALVVGLAVGNLVLVNGTVLTAIGLSDSDDGLAALLLPIAAGIGL
                     ILYGYKQFRYGEEVEDKPNEFQKAATGPGKGFFGGSTPDLDLQGMLKTGMTLLEDLEE
                     ASQNSDTDD"
     gene            complement(19450..20262)
                     /locus_tag="PLC06_00985"
     CDS             complement(19450..20262)
                     /locus_tag="PLC06_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89047.1"
                     /translation="MAETVTTTQLLCKQCAAVLVVEQGAQYATCEYCGTVNFLDKSES
                     VLHYAVRPTIDDAQATAALRRWMAGNHTVKGLDAEAKIESQRFEYFPMWLARTEMSGR
                     EEVHLKPAAALSIVELGNLRVPASDLEPYDPALDSTAIRATVPVTAVRSWLTDNERIA
                     PGAIREVSLVHVPIYQFKYRYRENTYTALVDAATSQVFSAIYPEKFELPYVSIGGFGC
                     LAYFLAALIPLISYSVFDGGEGAAIGTAIYLIVAVALAIPIFIAAAAISRKY"
     gene            20501..21058
                     /gene="efp"
                     /locus_tag="PLC06_00990"
     CDS             20501..21058
                     /gene="efp"
                     /locus_tag="PLC06_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRO89048.1"
                     /translation="MIDVNQLRRGVTFLHDGALLKVTEYSHTKPGRGKATIRVKVRDL
                     RTGTNKEVTFSSGDRVDDVRLDKSAFQYLYDDGTFYVFMDLSSYEQKQVVHSVLEDDA
                     KFLRDNMELELLSYEGEVIDYELPKTMEFDVVEAENAVAGDTATGATKEIRTDTGLRV
                     KTPLFVQVGDRIRVNTDTGEYLTRV"
     gene            21146..21604
                     /locus_tag="PLC06_00995"
     CDS             21146..21604
                     /locus_tag="PLC06_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO89049.1"
                     /translation="MSLNKAAVRLVAVVMLMMLFLVATRPAEAQASGVYHIVQRGEYL
                     SVIAQRYGTTVQAILTANPTITNANLIFAGQTIFVPFGAQPPQPTPVPPPPTPVPPVT
                     PACRWNHLVVPGDTMLKIGRLYGVDPFVIARANNIFNLNLIFAGSTLCIP"
     gene            complement(21712..24225)
                     /gene="leuS"
                     /locus_tag="PLC06_01000"
     CDS             complement(21712..24225)
                     /gene="leuS"
                     /locus_tag="PLC06_01000"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRO89050.1"
                     /translation="MTTQSKSRSKPYDFRALESKWRPRWRAADLYRTGDDPDKPNYYI
                     LDFFPYPSGDGLSVGHCRNYVPTCVSARFHRMRGYNVLHPMGWDAFGLPAENYAIKHG
                     IHPVETTQRFVANYRRQMDLVECSYDWSREINSTDPAYYRWTQWLFLLLHRRGLAYRA
                     SGSQWWCPQCRTILANEQVENGRCWRCDSVVERKQLEQWYFSITDYAERLLSGLDTID
                     WPDRIKEMQRNWIGRSEGAEAVFMADGHPIPVFTTRSDTLFGVTFLALAPEHPLAAHV
                     TIPDRAAEVNAYRVAAGRMSEIERQTTDKEKTGVFTGSYATHPLTGEPVPIWVADYVL
                     ASYGTGAVMGVPGHDARDFAFARRYRLPIIAVVEERRPTTDGRPQTDSDSQPSTVVDQ
                     VTSVFEEPGIVINSGPYSGLSSAAASAKIAAELAARERGGPRVAYRFRDWLISRQRYW
                     GAPIPIIHCSACGPVPVPENELPVLLPDITDFTPSDDGRSPLARAEAWVNTTCPQCGG
                     PARRETDTMDGFACSSWYFLRFANPHIDDRPFDPAAVSHWLPVNTYVGGAEHAVMHLL
                     YARFWTKVIHDAGLIDFDEPFTQLRNQGMLLSPYDGQKMSKSKGNVITPDEVIAEYGT
                     DVLRAYILFLGPFDANALWDNRNIVGVQRFIERYWTLAHEMASHPNSGQAINEEFERT
                     RHKIIRRLTGDMSRFRFNTAVAALMEYVNYLYAARSWPIPGDQWRAAIRTLTLLLAPI
                     TPFITEEVWRDVLGERKSVHQADWPAYDEELARDEEVTIIVQVNGKLRDRLTMSAGAD
                     ETTMREETLANEKVRLTIGRRPIIDVIVVPGRLVNVVTG"
     gene            24894..25718
                     /gene="cdaA"
                     /locus_tag="PLC06_01005"
     CDS             24894..25718
                     /gene="cdaA"
                     /locus_tag="PLC06_01005"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRO89051.1"
                     /translation="MELFESLLERLSLIGIVDIIDILLVTLIIFGVLMLIRGTRAVQV
                     LRGLLVLGAIVFLLAQIFELRAFSWLVDNMLPVLLIAIPVIFQPELRRALEQLGLAGR
                     YLRLFKRDDTNPVVEALKEAALRLSQRRHGALVVFEQDTGLQEYIDTGVILDADPSPE
                     LFLTIFNKNTELHDGAIIIRENKLIAAACVLPLSTSSLSDRQMGLRHRAALGISEVSD
                     AVAVVVSEETGQVSIAHNGRILRRQDIARLDTILHAFLTGPRQPGFLRRDSSEPSG"
     gene            25702..27093
                     /locus_tag="PLC06_01010"
     CDS             25702..27093
                     /locus_tag="PLC06_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRO89052.1"
                     /translation="MNRAGETLTNVATFILSLILAIFIWTSASQSQDPIRTRFLEVPV
                     QYVGLPENTTLVDVDPRQTVQIRLEGPDTILQKYTPEDFSATVDLSQVPSGERVSVDI
                     NVTTTKTGAEISFITPEQVDVTLEEQVTRDIPVELDIRGSVARGHSQGEPLIEPPTIR
                     VSGPASQVNQLNFALVTIFLNSAVETLVETSTPLFYDLAGRVSSVNALALSHNTVSVT
                     IPVEELAGFADKLVTVVWTGEPAPGYRLLSVTADPPSLLVEGRPAQVNALNTVSTEAI
                     DIDGLTESFQQTAVLDLPPGISVDAEQTVSVKIEIAPILTTSSFNRVPDPRGLRAGYE
                     VKVEPEQVRVVLFGPLPLLDSLAENDVRVILDLFGLEPGTYTVKPEVDIPEREIEIRS
                     IQPSSVTVTISQTVPSTTSLLDGQLVAAGQVPLSGSPPVRPARVPTNYYPMSWGAIAS
                     RLPELYLERRGFE"
     gene            27090..28466
                     /gene="der"
                     /locus_tag="PLC06_01015"
     CDS             27090..28466
                     /gene="der"
                     /locus_tag="PLC06_01015"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018976390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRO89053.1"
                     /translation="MNQKALVALVGRPNVGKSTLFNRIIGRRLAVVSDVAGTTRDRLY
                     ADGEWGGVAFTVVDTGGIEVTEGRNTAPLSEDSEQFLPLIRQQAVIAMQDADVIVQVV
                     DGQTGITAADREVASILRQAKKPVIVAANKLESSKLWDTAYEFYELGLGEVFAVSALH
                     GAGTGDLLDAIVESIPPFDPDDDEEDTSIKIAILGRPNVGKSTLLNKMIGEERVIVSP
                     IAGTTRDAIDEKLRWHGQDFTIIDTAGIRRRGKIDQGIEKYSVLRAIKTLRRADVALL
                     LIDGEEGVTAQDAHIGGMLVEENVGVILIVNKWDAVEKDTHTMPAYETDVRRELAFLS
                     YAPLLFISAETGQRVNKILETVMAVYEARQTRLSTSEMNDLMRDIVAHHPPPTKGGTQ
                     LKFYYATQVAVAPPTFVFFVNRPEMLHFGYERYMENRIREQYPFTGTPIRLLFRGRTD
                     ERKPGQPK"
BASE COUNT         5791 a         8125 c         8892 g         5710 t
ORIGIN      
        1 cggccgtcat ctggtagatg cggccgtcga gcatgaccat ctcgccgtcg agcagatcaa
       61 acgtgcccag cccaaaatcg ccgtggcgcc tgatctcgct gaaggggaca tgctcctcgt
      121 agatgccttc gacgatggcg ttgacggggg cggagagata gagtcggccg ttcgtagtca
      181 tagggcttac ttcggctcgg aggcaacgat gacggggcca accgaccgct gttgctcgcc
      241 ggcgagatgc gggatgatgt tgtcattgaa ccaggtcggg ccgaagactt tggaaccgtt
      301 gatcgcactg agagcatcct cccataagag gattacgatg gctcgatctt cggccgactt
      361 gaccaggtag aagtcccgaa atccgggcaa cccgaccaga atcggcattc cctcattcat
      421 gatgctatcg cggatttcat caactggttt gttgaaatag agatggttta cgactgcgaa
      481 catagcaaat gacctcctcg aaatctaaca gattgtcaat aaggttttac aattcgtatc
      541 tgcagcgatc atatctgcaa cgacactggc ttacagatac cccaacccgc gcaaccgctc
      601 ctcaatttgc tccgcttcgg ccgcatcgaa cccctgctga gcggtcatat tcgcatcgtc
      661 atcttccgag aattgtaccg ggtttgactg cgtgaaaatc tcatccagca cgcggccgtc
      721 catcactcgc ggtacaggct cacccagcag atgcataata gtcggggcca ggtcgaggat
      781 attgacttcg ggtagctcaa cgccgttgtg gatggccggc ccggcagcga tgaagatgcc
      841 ctcgcgccgg tggcagccgg agtcgccgcg aatctgattg gtcatcactc ggccctcggt
      901 ggcgaagagg gggcaggcga tcatggcata atgatccagt tccaggtgta ggtcggggcc
      961 gagatgggcg taggggccgt ggtagacctc gcggccgcgc cacaggttag tcaggatggg
     1021 ccggccgtca tcgtcgcgca gctcggtcag cgcgtcgctg acctcggtca gccgccgctc
     1081 gtaatcggct tcggcgacga tgccgtggcc gtctctcccg atattgttta tgtaaacttg
     1141 tcccacatgc cccatgctgt aggcgatcgt ccggctccag tcgacgctgt cgaagctgag
     1201 aaacttgccg acgatgtcgt tgcgcgcctt cttgctgacc tgggcgacgg tgttttgcaa
     1261 gcccgcccgc gcgatccact gataggcgcc ggccggggtg atgccatgac tgaaggccca
     1321 cgccttgagt tgggtcacgg cgtcaggctt cagcttcatc aggccgcgct gcatgaagaa
     1381 gacattgagg ttgaccatgt tcttcaacgg gccgaagccg tggtcggaca tggcgatggt
     1441 ggtggaaccg gcgggcagtc gcgccatgag ttggccgatg gacgcgtcca ctttggcgta
     1501 gccgtcgcgg atggcgtgtt cccagggcga tggctcatac cgttcgtggt cgtgatccat
     1561 gaagcgccac agggcatgct tggcgatgtc catggagatg aagtggacca tgagcacgtc
     1621 caccggctcc gactccagca gctttagcgc ccagcgggtg tgggcgtcga tgaggtcgta
     1681 gatatcggca atgaattcgg cctcgttgcc cgccttgtac tggatgttcg gcgcgacgcg
     1741 gtaaggcccc aactccggtt cgtagcgact gatgagatcg gccgggtagc tgatggtcgc
     1801 gttcttcggg ctgaggatgt cggtcaccat gaagccgttg acttcctgcg gcgggtaggt
     1861 caccggcacg ttgagcacgc cgacgcgata gccggcctgc gacaaccttt ggaagatggt
     1921 cggctgacgg atctgggtcg aattgacgag ggtgaagttc tcgccgtggg gctggatgaa
     1981 atcgaagacg ccgtgcttgc cggggttgac gccggtcatg aaggtnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnngctgg atgaaatcga agacgccgtg cttgccgggg
     2161 ttgacgccgg tcatgaaggt tggccaggcg gggctggtga cggggggcag ggtggaggcg
     2221 agatcggccg tcacgccgtc gcgcatcaat ccagccagat tgggcagaaa gccgtcgcgc
     2281 gcccagggtt tgatcaggtc gaaggtcgcg ccgtcaaagc cgatgatgag aagggtcatt
     2341 gtatcgcttg cagtgctggt agtggtgtac cggttgctac gctgtaaagc acatccggat
     2401 caaggtctac gccattaggc cagaccaatg tatgtgcttc tgggtccaca gcgacacgtg
     2461 cgaagtattc tatcttttcc aacggactaa acacgccgcc gcgattgcga atgcgcgact
     2521 ctaaatctat gtccgcctct tcaccgttcg taaatttcag atgcaaaata taatctcgaa
     2581 cgtgttggac gtgggttatt cttggaaaca tgatgctcct caaatgtcta atcaagcggc
     2641 tcgatcctaa ccatcgtttg attgacctca agccgtttcc agttatccat tagctcctgc
     2701 tgatgaatgg ctgcccattc aataaccatc gaaatcgccc ttctaggcaa ctgcccgttt
     2761 agaattacga tcgggtcaat gctgacctgt atttcatatt gcccataaat tgcatgaaag
     2821 tgaggtggtg gatgatcgtc ataatacatc cttatgatga taccgaaaaa gcgacttaac
     2881 tcaggcattt gaatagtata tcatgctcat ttgagctgat tgaatgtgct tgactcttgg
     2941 ccgatgattt ctctcacgcg atagacccgt aaatcctgcg tctcatagta tgtgcgcacg
     3001 gttagctcgg caataaggcc catgatgata aactggacgc cgatgatgac cagcagcacg
     3061 ctcagcagca acagcggccg atcgccgatg gtcatggcgt tgaagccctc gatgccgccg
     3121 gtgatgcccg cccagagctt gagcgcggtc agatagccgc cgatgagcgc gccgagagcg
     3181 aagaggatga tcccccagcg gccgaagagc tgcatcggcc gatcgccgta tttgcgcagg
     3241 aagtggatgg tgaacaggtc gaggatgacg cggaaggtgc gcgagaggcc gtacttcgac
     3301 gtgcccgcca cgcgggcacg gtgattgacc ggcacctccg acatggtgaa tccggcgaag
     3361 ttgaccagtt cggggatgaa gcggtggagt tcgccgtaga gatgcaggtc gcggacgacc
     3421 tcggaccgga aggcgcgcaa cgagcagccg cgatccttca ggtggacgcc gctggtgcgg
     3481 gcgatgagcc aattggcgac gcgcgacggg aagcggcgca ccagatcgtc ctgccggttc
     3541 tcgcgccagc cgttgacgac atcgtagccc ttgtccagtt cggccagcat ggcagggatg
     3601 tcggccgggt cgttctggcg gtcggcgtcg agggtgacga tgacgcggcc gcgggcgtgg
     3661 tcgaacccgg cggcgaaggc ggccgtctga ccgtagttgc gccggaaacg gatggcgacg
     3721 atgcggtcat cttcggccgc cagttccgcg agaacggtga agctgcggtc cttgctgccg
     3781 tcgtcgatga agatcgcctc gtaatcgagg cccgtgccgt ccagcgcgac gcgaatctcc
     3841 tcggccagcg ggcgcagatt ctcttcctcg ttgtagacgg ggatgaccag gctgaggcct
     3901 tttgtggtga tcggtgtcaa tgcttaggct acttccagtt cataccgttg aataaggatt
     3961 tcggctggta tacctaaacc ctctcgcagg tttctgatca tttcaagaga aagagggata
     4021 cggcgctcca gtactgcctt gacttggcct cggcccagat agggttcaag ttcatcgaac
     4081 gataattcct ggctctccag gtagtggtga atagcctcaa taggatccgg cgcgtcgatg
     4141 ggatccgtcg cttcctcgta ggcatcgacg agggtgatca ggacttccaa cctgtcttcg
     4201 tctgttgcat cgggaggcgc attcagtaac cgatcaattt ccttgagcgc gcactggtaa
     4261 tccgcttcat tacgaattgg ttttacttcc atcgatttag tccttgtatg cgaaagtgaa
     4321 gcttgggtac agataaattg tcagccttac ctcgattaca aattcgaact ataccgttga
     4381 cacgtcgata tcgtcatact cgcgatgagt tccaatgaaa cgaacataga cggcatggtg
     4441 tcgatagaca actacgacta ccaaacgata gccgttgcct ttgatgttga agacgacgcg
     4501 atcgttgccg atgaagcttg ccgtgcgata ggcttgctta acatcggccg gtgtctgcca
     4561 atcggcgttt cgtactctag tataccatgt tcgtaatggt tgttcggcat ccgggtgttt
     4621 ttcccagaac tctcgaagcg tgcgacgggc gatgatcctc atagcttcta ggccacatac
     4681 cccaaatcac gcaacttctg catgaccagc tcttcttcgg cgtcggtgta gatggcgtcg
     4741 ctgccgacgc catcgccggg gccgatctgc gagtaggtga tggggttggc ttcgttgaaa
     4801 acgtcggtga aggcgtcggt caacacgtgg ccgtccatgt attcgggcac gggcaagccc
     4861 aggtaatgga gaatagtcgg cgcggggtcg agcagactag ctccttcgag cttcacgcca
     4921 gggcggacat ccggcccgcg caagccgacg acgccttcca tgtggtgatg gccggtctgg
     4981 cccgtgctgg gttcgatgat gcgattggag ccgaaatcgg catggccgaa ggagacatat
     5041 ttggcccggt cggtatggag caccagatcg ggcagccgtg ccgcgctgat gccgtggaat
     5101 acttcctcgc ggcgatagac cacgggcacg acctggctgt tgtctttcgg gtctcgtaat
     5161 tccttggccc tggcgatgat ctcgtcgcgc aaggattcat attccgccgg gtcgaccgcg
     5221 ccctgcggcc gttgaccctt cacgttcagg tagacctggc cgaagttgcc gacggagaag
     5281 gccttggtgc gtgaccagtc gacgtcgttg aacgacagga agaagcgccg cagcgtcttg
     5341 ccgccgcggc cgcgcttcac gttcttgcgc atgtcgctca gtcccaacgc tgtcagccac
     5401 ttgagggcgt tgatcggtgt cacgccgagc ttgaaggcgg cgcgcttgag ccggctcatc
     5461 ggcgagcgct tgaagaccag ccagccgttg tcggccagcc agttgttgat gtggaagaac
     5521 ttgtggaacg ggccgaaacc gtggtcggag aggacaatga ccaacgcctc ttccggcacc
     5581 agcgccacca tctcgcccaa caggcgatcg acggtttcgt agtagtcaag aatgtcgggc
     5641 aggacgcgga tggccatttc ggggtcgtgc attgggtggg atttatcgac cagatgccag
     5701 acctcatgtt gcaaattgtc ggtcgtttcc atcacatagg cgaacagatc ccagggcttt
     5761 tcgcgcagca ggtattggac gttacgcgcc cgatcgattt ccagttgctt gcaggcgcgg
     5821 acgaagacgg ccgggtcgac gccacgcccc ttttccgaca tgcgcagcgg gaacgggccg
     5881 caggcgtcga ttagctcttg tttgagttcc ggtggccagg tgtagtcgct gtcggcgctg
     5941 ggcgtcagga aagagcagag cataaagccg ttcatcggcc gcggcggata ggtcatcggc
     6001 aggctgacga cgcccacctg cttgccggcc gcgccggcga tctgccacac agcgggcacg
     6061 gcgacggtca ggccgttgga cacccgaatc tcgtagctgt cctgggccgg gtaggtgaag
     6121 tcgatcaggc tgtgctggcc ggggttagtg ccggtggcga aggacggcca ggccgaagcc
     6181 gagacgggtg ggatggtcga gcgcaagcgg ccgccggctc cctcggccaa caatcgggcg
     6241 aggttgggca gatggccctc gtcgagccag ggttgcagca gatcaaaggt gatgccgtcg
     6301 agaccgaaga cggcgacttt tcgttgggtc ataagtggtt cggtggggcg gggctaaagc
     6361 taatcgtatt cgcccctaga gcttgtgggc ctccggtggt cgagcgaagc tcaggatgct
     6421 acggaaggcg tacatcaggc cacccagcag acccgcgctg aagcgcagga aatagaaagc
     6481 cagcgacatg gagacggccg tggccgccgg aatgccgacc gggacgaaca agaacgtata
     6541 aattccttcg cgcaggccaa ggccattgaa ggtaatgggc agcaggttga tgatggcgat
     6601 gaccgggaca aacagggcga agatgcgaaa gggcaaatgg acgccgagtg ccagagcgac
     6661 cgtcatctgc accaggatca gattcagggt aaagggcagg ctgatcagca gcgccgaaat
     6721 caaggcacgc aagggatagc gatgaatggt gttgacgagg ttctcgattt tgttatagaa
     6781 gggcagattg cccgcattcg gatagacacg attgagtagc ccgatcgggt tggcccatcg
     6841 ggcgatgaag aaggccagcg ggatgccgac ggccgtcacg acgatcagcg cccacaggat
     6901 ccacggcagg tcaagggtcg tggcatggac ggccagattc catgtcaagg cgatcagggc
     6961 taccagcgat gacccgacca ggccggtgat gcgttccatc aggacggaac tgagcgcctc
     7021 ggcaccgtgg ccgtggctct ggcgcaagct gacgactttc accagatcgc cgccgaagcc
     7081 ggagggaatg aagttgttgg cgaagaaacc gatgtaatag aggtagatca aatagccgaa
     7141 gctgggcttg tcgttgaggg cgcgcaagag gacgtaccag cgataggcgc ggatgaccgc
     7201 gtttaattgg aatagcagga aggcgagggc ataccatccc caatggatat tggcgagggc
     7261 ctccatgacc tcggtcaacc caacctgacg caatagccag accagcagga tgatgctaag
     7321 cagaatttgg gagaggccaa atagtttctt gcgattggcc ttcaggaatt tgcccataac
     7381 caccggatta tagcacaagg ggttgggacg cgataacgat acagctacga gacattgacg
     7441 ccaagccgcc gcgaccacgc tgtgtaccga tgccgaggtg gactaatagt cggaccgact
     7501 acttgttcgc cgggcgtaca agtacggtat atcttactga aaatgttcct cgttagacag
     7561 gaaccagtat agtaagaggc ccagcggcac aaggggcgga caagcgcaga ttatggcgag
     7621 caagtccgcg attggcacaa ttctctgtgc ccaccagtaa cccgccacgg ccagaggaac
     7681 gatgaacaga ctcacgccca gaaggaagat ggtaaacaat cgcgcccact ctttcaaccg
     7741 ccaaatccct tgggccatga tgacgcagat gactgctacg atgacaggaa caggccacat
     7801 cacactaccc ctgacgatta caaaaatgca taacccggcc aatatgagaa gaaatccggc
     7861 gaaaaagatg accactatcg gtcgaataag gcgatcagtt accaccttat ccatagtgtc
     7921 ctccctccgt tagcgtccga tgtacttcac ttgcccaggt tcgtacgttg acgacatagt
     7981 tttccggtcc acccgccacc acgtcggccg ccgtcattgt ccaacggatc ggccggtcat
     8041 aagcgccctg attgccgggg cgggccgtga tcgaccagtc gcgcgcgccc gaagccaccc
     8101 gcccgctgtt cttctttctg acttcttcgg gactcaggcc gtgctcgatg aaatcggcca
     8161 gcaatccccg cgcgacagcc aacccctcgg ccgagtagag gccgggatgc tgcaaatggt
     8221 agcacagcac catcagatga tggacttccc atcgcgtcgg gttttcgttt tcccagaaga
     8281 gcatttgatg aaagtggtcg cggcaggttt ggtttatcgc ctgaggcgcg ccacattcgg
     8341 ggcattgatc gatcatgatg agattgtctt ttatatcccg atttatcttc ttatactgta
     8401 tcttatagaa agaatcatca acagatttca caggtatgaa aggagcgaca atccatgaga
     8461 gcaatccgcg tccacgaata cggcggcccc gatgtgctgc atttggaaga gatcgatatg
     8521 cctgaacccg gctgggggga ggcgcgggtc atgctgaagg ctgccggcgt caactttgtc
     8581 gatatctatc agcgcagcgg tcagtacaag atgaaccttc cctatacgcc cggccgcgag
     8641 ggcgggggca tcgtcgacgc cgtagggcct aatgtcactg atgtcgcgcc gggagattat
     8701 gtggtcttcg ccatgcacct tggcgcgtat gcggactatg ccattgtgcc ggcctggatg
     8761 ctggcgccgg tcccgcccgg catcgatatg atgtcggcga cggcggccgt gctgcaaggg
     8821 atgaccgccc actatctgac ccatgacacc taccccatca agccgggcga caccgtgctc
     8881 atccacgctg cggccggtgg caccggctta ctgctggtgc aaatggccaa gatgcgcggt
     8941 gcgcgcgtca tcgggactgc ctccaccgaa gaaaaggagc agctggcccg ccaggccggt
     9001 gcggatgaga tcgtccgcta tgccgagcag gatttcgagg ccgaagtgaa acgcctgaca
     9061 ggcggccgag gcgtcgatgc cgtctatgat tccgtcggcc gcgacacgtt cctgaaaagc
     9121 ctcaactgtc ttcggccgca cggctatctg gtgctgtttg ggcagtcgag cggcgccgtg
     9181 gcacccttcg atcctcaaat gctgaaccac gccggctcgg tctacctgat ccggccgtcg
     9241 ctgcatcatt acatccgcga ccgcaaggag attttgcgac ggacgagcga cgtctacacc
     9301 tggttgggag acggccgcct gacggtgagg atcgacaaga ccttcccgct ggaagacgcg
     9361 gccgacgctc atcgttatct ggaagggcgc caaagcaagg ggaaggtgct gttgggaacg
     9421 cagatgggtc gggaattaaa agtcggcctg ttgaacagag cgattgagcg cggcgatgtg
     9481 attgacgaag cggggtggga atctttcccg gccagcgatc cgcccgctta ttaatagcgc
     9541 agggtggcgg cctgcttcgc cgccgtgcta ttttttcagc accgacgcca gcccccacag
     9601 gctgaccaat gcccacgtcc cttccagaag caggaagccg tattgccgct cgacgagggc
     9661 cacggcggcc agcgtgccgg atccggcgag gttcatgagt tgatagacaa agctgtcacg
     9721 ggtgagtcgc tcaaattgat tggcagcgaa cgggatgagg atcagcagcg caccgataac
     9781 cgagataacc tgaatcatag ggcaaatact cctgacttat aagctcgccg ggcacgccgc
     9841 ggccactatt atcactggcg cgacgaaggc gcggggaatc tgacgacgaa gaccgtcccg
     9901 cggccgacct ggctcgaaac gccgatcgtg ccgccatggg cggtgaccag ttcgtggacg
     9961 atggccagcc ccaacccgga gccgcggccg tgctcgccgc gcgcgcggga tttctcaagc
    10021 cgatagaaac gctcgaacac gcgcggaatt tcttcttcgg ggatcccttg cccggtatcg
    10081 ctgacgatcc cttcgacctg gttgccgttt aatcgaccgg tcaatcgcac gatgccgcct
    10141 gccggcgtat aggccagggc attatcgacg aggttcgtga atatctgcgc cagccgatcc
    10201 gggtcgccca tgacgaacgc ttctccggcg acgccaagcg caaggtcgat ctctctggcg
    10261 cgcgcgttgg gcaactggct gcgatggacg ccggccaaaa tgtcgtttag atcgacgcgc
    10321 tgcatcgtca acggcatttg gcccgattca aggcgagcca gatcgagcag ctcgttcacc
    10381 aggcgggaca tgcggtcggc ttcggcatgg atggtttcgg ccgcccgttt gcgctcctct
    10441 gggccttcag ccgcgccgtc gatcagtgcc tggctccagc cggagatcgc cgtcaacggt
    10501 gtcttcagat cgtgggagac attggccaca aaatcgcgtt gggcttgttg agtggttttc
    10561 acctgggcgg ccatgctgtt gaagctgccg gccacgcgtc ggacttcatc cggcccttcc
    10621 tgtgggacgt gctggtcata atcaccacgc gccacggctt cggccgcgac ggctatcgcg
    10681 cccaaaggcc gggccacgga ttgggcaatg atcgctgcca gcagcatcgc caacaatacc
    10741 gccagtacgc ctgcattgaa gagcggccgg agaaagaatt cgttgaaaaa ttcacccgcg
    10801 gtgggttcgg gctgggcgta gaagatgagg gcgaggccga gtgcggggtc cggctcggcg
    10861 aaaaccagcc agcgcgagcc gtcagtgtgg acgaacgtgc cgaagatgct gccgcgtctg
    10921 acgttggtca aaaccagcga ttgcggtcga tctacgttgg tcagccggtc gcccacccaa
    10981 tcgtcgccga cggccgtatc gaagacgatc tcttcggcgc tcgtatcgac gaccagtatc
    11041 cgcacgtcgg cctgttccga ggtgttatgg agcaggccct gcagctcgga gctgccggcc
    11101 cctgcccccc acagttggag taattcccgt tggttggtgc ggctgatggc cgaaaggcgc
    11161 tcgagcgacg gcagcaagcg ggcgtcggac acgctggcga agccaaacag cgagacggcg
    11221 acgatcagca gtccgacgag aatgactacg acgaatgaca gcagcaggcg accgcgcagc
    11281 gttcgaaaca tggtatgaag ttagcgcaaa ggggcaaaga cgtaaaggat tgacaatgag
    11341 gattaaggag aacaggggcc ggactgatta acgacccggc ccctgatcaa gcttatatca
    11401 gtctctcgcg cgctttggcg ctcaggtaat ccatcgtcca gaccatgatg acgatggcca
    11461 ggatggccgt accggcggct ccgtactggt tcaggctgac ccagatgcgg aactgctggc
    11521 cgatgccacc gccgccgacg aagccgatga cggtagacat gcggatatta atgtcccatt
    11581 ggtagatgat gaaggccagg aaggggggca ccaactgtgg cacgacggcg tagaccaggg
    11641 actgcatgcg cgtggccccg gtcgaagtca cggcttccac cggaccgctg ttgatctcct
    11701 cgatggaatc ggagaacagc tttcccagat tggggatgtt gttgagcgcc agtgccagca
    11761 cgccggcgaa cggccccgcg ccaacccagg tcgccgcgat gaggaccagg atcaacggct
    11821 cgatcgagcg tgcgacattg aaaaatgtac gcaagccgta ataaatggtt cgaccggccg
    11881 gcgtatcgcc catgatgttg cgcgccgcca ggaagctcag ggggatggcg aatagcgcgc
    11941 ccagcgtggt ggcaaccagg gccatgagca gggtgacaat cgacagcctg atgactgttc
    12001 tgacggtctc gctgagctgg atgcggccgg caatctcttc ataggtgatt tcgacgcgag
    12061 ccggttctgt ctgcccttct tccatgacga caatcgggtt gatcgccgtc tctacccgca
    12121 gattgccgtc atcatcagta tcgcaacagt tcgagcggac gcgcaaatac ccgccatcgg
    12181 gcaagaccca gcgaacggaa accgtgcggt ttggcggcag gcccgttccc tcgatgacca
    12241 gcggatcgcc ggtttctccg cagtcagccg atagtgtgat ggtcgcgtcg gccggcgtcg
    12301 aaggctccgc cgcgccacaa ggtacggtca atggggcgct gatggccacc gtcttcgtgg
    12361 ggcgggtgaa tagctccggc gtggcaaact cgcgagcgag cgccaacccc tgtggcgcgc
    12421 ctttgaccag ctcgatcagc ttgatttggg ccacattcca tgaccagata aacgtagcca
    12481 cgaagccaac gatgatgact gtcttaagga cggtgcgaac cagcggattc gtgctggaaa
    12541 tgatcggccg cccctggatg attctttcgc gcagccaact ggacaggaag tccagtatgg
    12601 cgacgacgag gatgatggcg atgagggccg tggccatggc tgagaaacga ttcagccgca
    12661 cccattgaac cacgaggaaa cctagtccgg catcgctcac gaggccgatg accgtggaca
    12721 gacgcacgtt gatatcgaac cggtagagcg tgaacgaggt gaatgtgggc aggatttgcg
    12781 ggaagacggc gtagatgacc atctgcggcc agctggctcc ggtggctttc accgcctcaa
    12841 ttggccccga atcgatgctc tcgatggctt ccgaatagag cttggccaat gcggccacgg
    12901 tgtggaacca caaggccaat gtgccggcaa acgggccgag gcccacccat acggcgaaaa
    12961 tgatggccca cagcagggtc tcgatggaac ggatgacgtt gagaatggcc cgcacgacgt
    13021 tgtagatcag catcgtgacg gggttcccgc tcatcagatt gcgggcggcc aggaagctga
    13081 ccggcacggc aaatataacg cccatcaccg tggccagaaa ggccagggcg atcgtctcgc
    13141 cgatcttctc gatgaccagc gacagggttt gcgtgggttg cagccggcca gacgggatgt
    13201 tctgcaccgc gcgaacggca tgggtcaacg tctcgccggg agcaggttgg gcggtcaggg
    13261 ggacggcttg gggaacaagg agcgtcacgt caaaacggcc gttctcatcc gccttgaaag
    13321 tgactacttc gccgtcgaat gtagcccgac gaggggatcc cagcggatca atccattgca
    13381 tctgccccga ttcatttggc tcgaagcctg cccccgtcac ctgcaacaaa tcgccgacct
    13441 cggtgcaggg cgcgctcagg attaattgag gggacgtggt tgcttcacgg ttcggagccg
    13501 gtaacggatc gacgcagggc accatgatcg gcgtcacgcc gaccacctct ttcatcggcg
    13561 tctcgaataa ctccggatta aacagagatc gcagatacgg agtgacgttg ggcaaccccg
    13621 ttatcattcg ggtgggacgc acttcggtga cgatgagcgc cgtgccatag agaacgatgc
    13681 caatgagcag caacgataca ccccaactcg gctcactccc tttggccaat cgataggcgt
    13741 cgcgggcgat ccagatgagg agcagggcca gtggagccag aagcgcgtaa gcctgagtcc
    13801 acagaaccag gcccagcagc gcaaaaaaag ccagaaaaag agcgaagccg cgagtcttgt
    13861 ggctaaggat aatctggccg ctgccgggaa tgatcagaga gaggaaggcg ggcagccaag
    13921 ggctgagaga tgtcttgttt tcagacggca ttaggcggcg ctaccgatac catttctgcc
    13981 tcctccccgt acacctcttt aaatttctcg ggcgtcaatt cggaagggag accatcgaag
    14041 accagttcgc cacctttaag gcccacaacg cgggttgcat agcgatggac gaggtccaga
    14101 aaatgaagac tgcacaacac ggtgatgcta tcctgctgat tcagcagttc cagatagcgt
    14161 aaaatggagt gcgagaggac gggatcgagg ctggcgacgg gttcgtcagc cagtaatagt
    14221 tgtggctctt gcatgagggc acgagcaatg ccgacccgtt gctgctggcc accggaaagc
    14281 tggtcggccc ggctgtcggc cttatcggcg atgcccacgc gatcgagcgc agccagggcg
    14341 cggcggcggt catcggccgt gaagcgccca aagagactcc atacgggatg tacataaccc
    14401 agccggccgg tcaggacgtt ggtagtcacg ctgctgcgct tcaccaggtt gaaatgctgg
    14461 aaaatcatgc cgatctgccg gcgaatttgc ttcagttcgg cgctgccggc ttcggttatg
    14521 ttctggccat tccagagcac cgcgccggaa gtgggttcga tcaggcgatt gatgcatcgc
    14581 agaagtgtgg atttcccgga gccggagagg ccgatgatga ccaggaactc gccgtcctga
    14641 acgtcaaaac tgacgttgtt cagggcgacg gtcccgttgg ggaatatctt tgtcaggttc
    14701 ttgatttgca gcacgacaat tctcttgtgg gtcctgcctg attaaagaac ccccggcagc
    14761 catcggactg ccgggggttc aggttcaaga cagggcttac ttggccaacg actcgatgtc
    14821 gatacccgcg cggctcaggt cggcgcggaa cacatcgtag aagctgtcgc tcgattcgac
    14881 gagactctcg atgttataga gcgtgttgag cgcttcctga ccctcgggcg aggcggagat
    14941 gtccagcaga gcggcgacga tcttcgtgcg catctcggcg gggaaatcct tgatgaagga
    15001 aacgctgtcg ttggggatgt cgctggtggt ggccagcacg acgacctttt cgagcacatc
    15061 cgggaactct ttctcgatgc cggtacgggc gtcggagaag gtggcgccgg cgtcgcaatc
    15121 accattgtag acctgggtta cgacgttgtt gtgggaaccg gcttcgacgg tctggctgaa
    15181 gtccgtgtcg gggttgaccc cttcggcggc cagcatgatg cgcggaatga tgtagccgga
    15241 ggtcgaggcc gggtcgaccc agcacatgac cttgcctttc aggtccgcga tcgtggagat
    15301 gccgctgtcg gcgcgcacga tgatctggcc ggcatacgtg gacgagccga agcgagaggt
    15361 cgccagggcc gcatcaacgc cgcactgctc gttggccagc acgtagttga aggtgttcaa
    15421 ccagccgatt tgggccttgc cggcgcacat ggcctcgcgc acggaggaga agtcggtgcc
    15481 gacgttggcc ttaacgacca gaccggtccg ctcgcttacc atctgagcca gcgtgtcacc
    15541 actggcgatg atgtcctgcg tgtcaccgga tgggacgaag gacatgatga tgggattctc
    15601 tgcggttccc agctttggcg cgccgccgca cgcggcgagg gcgaacacca gaacggcaac
    15661 catgctaacc aacaacaata acttacgggc gttcattctt tacctcctca ggtgaaaaaa
    15721 aattggaaag agcctggtta aatagacgat cgtccttcaa ccgtctcata aggggatcga
    15781 gactccatac gagataaggg aatgataatt gtctactacc ggattgccaa caaaaaacga
    15841 agggaaatct caggttgata acttcctcag gttcacaaca gtgcggcagg agcgtggtat
    15901 cggaaagtca gcagctttct ttcctcgctg tcggcaaatt caaatgtggt gacggacgcg
    15961 ggcgaggggt atggctcgga tatgcccgcg tcgatcagcc gcttgcgttg gctcgcggac
    16021 agggaaagat cggcagccca gagaatggca aaggcgatgg gatcggcgtg gctgacgccg
    16081 acgacatgtc ggccggggtg tctttgccgg gccagatcaa agaacctgag caggcggtct
    16141 acgatgtcgg ccggctgctc ataaggcggg tttatctcat cgtagaagtt ccagtcgcgc
    16201 ttttccatct ccgccgccgc gagaccatcg aagggagaat ggatctcgtt gaggagatcg
    16261 cactccacga gcggcgcgtt ggccgcacag cgcgagcgca cgatctcggc cgtctggaag
    16321 gcccgcaaca tggggctgtg gtagactgcc acgatccctg cgcctgccag ataatccccg
    16381 gcagcggccg cctgcgctcg accaagggct gccagcccat atcccggcag gcggccatag
    16441 tagacctcgg cggggttatg aaccagccca tgtctgatca atgaaatttt cgtcataaag
    16501 tcgccaacct cacgctgcgc gcacgtttaa tttgtatccc cgcccccata ccgtctcgat
    16561 ctcgacgtca ctattcgtca atcgatcccg tagttgggcg acatggacat cgaccgtgcg
    16621 tgtctgtcca tgatattcat acccccacac cagatcgagc agctgatcgc gcgagaggac
    16681 ctgattggcg tgatcgatga gggcgaggag cagatcgaac tcccgggcgc gcagggcgat
    16741 ctcttcgtcg ccgacggtga cttcgcggct ggccgggtta agcgtgagcc gaccgagccg
    16801 tcgcggctga tcggggccgg ggcggccgcc gctcgtgcgc ctcaggatgg ccctcactcg
    16861 ggcgaccaac tcgcgcggat tgaacggctt ggtcagataa tcgtccgcgc ccatctccag
    16921 ggcgacgatt ttgtctatat cctgatcgcg ggcggtcagc atgaggatgg gcaagctgcc
    16981 ttcggcgcgg agcctgcggc acacttccca gccgtccagt tccggcatca tcaggtcgag
    17041 cacgatcagg tccggcgagc cactctgcgc ctgctccagt gcttgccgac cgttttcggc
    17101 cgtctgcacc cggaaacctt ctttttccag atacaggcga accaggtcgc gaatgttagc
    17161 ttcgtcgtcg gctatcagga tcgtagcagg catagttcag gttgagggaa agcgttcgac
    17221 gcgaatcatt gctcgcggca tgagtgttgc cgagggcacg gggccggtct gctttccctc
    17281 acaaatcatt ctgctattga gcgtgggcga tgcactcgat ttccaccaat ccgcctttgg
    17341 gaagcgcgcc aacttccacg gtggagcggg ccgggaacgg ctcggggaag tagcgggcat
    17401 agacggcatt catggccgcg aaatcgccca tgctttgcag gaagacggtg gtcttgatca
    17461 cgcgcgacag atcggaaccg gccgcggcca gaacggcgcc aagattgctc atcacctgtt
    17521 cggtctgcgc ggcgacgtcg ttgccgacca tctcgccgct ggcggggtcg agggcgacct
    17581 gcccggcggt gaagacaaag tcgccgatgc gaatcgcctg cgaatagggc ccgacggccg
    17641 cgggcgcgtc attggtgtgt actcgtttct tttccatgtg aatccttttc ctccgttatg
    17701 gtgagatgta cttcaataat atcttccatc tgtctttttg ccgctcggcc gggaaaagcc
    17761 aaagcgcctc gtcttccagc gatagctcga actcgaaagc ttccagatag tcggatcgtt
    17821 cggcctcgat ggtgaggacg accaattcca gcagcttgcg ggcatcggcg ggcgccacga
    17881 cggccggatc gaaaatcacc ccttcgggtg gcgtgccggc ctccagtatc ggttgctgct
    17941 ccagcaggga ttccaccata tcgcgggcct cgagcagatc gcaggcccac gcgggaatgt
    18001 agacgcggcg cggttcggcc cagaactccc gtgccactct ctcgctcgat tgaccagcct
    18061 gggtcttgcg cttcaggatc gttacccggc cgcggcagac ccaaaaaggc agccagcggg
    18121 gtggggcctc gaccgccggc gaaccgtatt gcgccggcag gatattcaat ccctcatcgg
    18181 cgatggcgac cgcgctccgg cagttggggc attggatgac ctgatcgtcc tgcccggggg
    18241 ccaaagggtg attacagcgg ggacaacgca ataacagcaa cttcatggca tcaatcgtcc
    18301 gtatcgctat tctggcttgc ctcttcgagg tcctcgagca gggtcatgcc ggtcttgagc
    18361 atgccctgca gatccagatc gggagttgac ccgccaaaga agcccttccc cggcccggtg
    18421 gccgccttct ggaattcgtt gggcttgtcc tcgacttcct cgccatatcg gaactgcttg
    18481 tatccgtaga gaataaggcc aatgccggcg gcaatgggca gaagcagggc cgccaacccg
    18541 tcgtcgctgt cggatagccc gatggcggtc agcaccgtac cgttgacgag caccagattg
    18601 cccacggcca gcccgacaac cagagccgcc gcacgataga gggtgttgcc cggcgccttg
    18661 ccgtacatca ccttcccgct ggttgcgtcc accaccactt gatagacgcg gttgcgatag
    18721 gagtagcgcg ccagccagac cggaaaatag accagcgaga actccggccg gagtagttga
    18781 atattttcgt agtgggtttt gctctgcccg gcggccacct ggctgcgata ggtgaagtgc
    18841 gcctgggctt catcgagggc cgccgtgcgc gactccgacg gttcgaaaac catcgcttcg
    18901 gcatggagtg cctggctgtc gaacggctgc aaatcatcac gcgagacgac gatgcgatgg
    18961 acgccgaact ccgacacgtc gactgcagcg tcgttccagt gcatcgactc gaagacagcg
    19021 tgctcgtcgg gcttcgtctc atccttgtcc ttgcggacgc gaccgaagag ccagcccgcg
    19081 acggtcgcct ccactcgcca gaacggcagg taagcgacca gcatctcgtc gattttggct
    19141 gtgcgtctga gattggatgc cttgcgcatg ccgcccagga atccgcgcac actctgctcc
    19201 gccgtggcac gatcgatgcg ccgtgccact tgccagcgtc gtatgccgcg atccccctgc
    19261 acaagggaat gaagactgca ataggggcat tgaacgatgc gcaccccttc cgcgacgggc
    19321 actatcccgc cgcaacccgg acagaccagc ccttgggcct gttcttttgg ctttatgttt
    19381 gcattcactg ccggcgcagt cgcagaagtt ggatcaggtg tcgtgagtgc agtcatggct
    19441 ttcaggagat taatacttgc gcgaaatggc agcggcggcg atgaagatag ggattgccag
    19501 ggcgacggcc acgataaggt agattgccgt gccaatggcc gcaccttcac ccccatcgaa
    19561 cacagagtag ctgatcaagg ggatgagcgc cgccaggaag taggccagac agccgaaacc
    19621 gccgatggaa acataaggca actcaaattt ctcggggtag atggcggaga agacctggct
    19681 ggtggctgcg tctaccagcg ctgtataggt gttttcccta tagcgatatt tgaattggta
    19741 gatgggcacg tggacgaggg agacctcccg gatagcgccg ggggcaattc tctcgttgtc
    19801 cgtcagccac gagcggacgg ccgtcaccgg cacggtggcc cgaatggccg tgctgtccag
    19861 cgcgggatcg tatggctcaa ggtcggaagc aggcacgcgt aaattgccca actcgacgat
    19921 cgacagcgcg gcggcgggct tgagatgaac ctcttcacgg ccgctcattt ctgttcgcgc
    19981 cagccacatg gggaagtact cgaaccgttg cgactcgatt tttgcctcgg catccagccc
    20041 cttgacggtg tggttgccgg ccatccagcg acgaagcgcg gcggtggcct gggcgtcgtc
    20101 gatggtgggt cgcacggcgt aatgcagcac ggactcgctt ttgtccagaa agttgaccgt
    20161 gccgcagtat tcgcaggtgg cgtattgcgc gccttgctcc accacaagga cggccgcgca
    20221 ttgtttgcac agtaactggg tagttgtcac ggtttcggcc atgctgctga tcctcaaccg
    20281 ccattgtagc aaaggcccgg cgtatggggt aataacctcg ttgggcctga tggatagtca
    20341 atttgggatg ccgttttgcg caagggattc actcgtttat ctatcagatt aggttaatca
    20401 cctgttcctc tgaggcgatt gccaaatgta gcggatttat gctaaactgt gtgaagtaat
    20461 tgacattatg tcttgtaaat taggagtaga atagcaacca atgatcgacg taaatcagct
    20521 tcgtcgcgga gtcacttttc tccatgacgg cgctttgctc aaagtaaccg agtattccca
    20581 caccaagccc gggcgcggca aggccaccat ccgggttaag gtgcgcgact tgcgcaccgg
    20641 cacgaacaag gaagtgacct ttagctccgg cgaccgggtc gatgatgtgc gcctcgacaa
    20701 gagcgccttc cagtatctct atgacgacgg cacgttctac gtattcatgg atctgagttc
    20761 gtatgagcag aagcaggtcg tccatagcgt gctggaagac gacgccaagt tcctgcgcga
    20821 caatatggag cttgaactgc tctcctatga gggcgaggtg atcgactacg aacttccgaa
    20881 gacgatggag ttcgatgtgg ttgaggcgga aaacgccgtc gccggcgata cggctaccgg
    20941 cgcgacgaaa gagatcagga cggatacggg attacgggtg aagacgccat tgtttgtcca
    21001 ggttggcgat cggatccggg tgaataccga tacgggtgaa tatctcacgc gagtctagat
    21061 tgatccgctg tgggatcggg gttggggccg gttttaataa tcgcggagat gcagtcagaa
    21121 tctggagggt aacatgagat cagtcttgtc tttgaataaa gcggccgtcc ggttagtggc
    21181 ggtggtgatg ttgatgatgc tgttccttgt ggccactcgc cccgccgagg cgcaggcgag
    21241 cggcgtctac cacatcgtcc agcgcggcga atacctgtcg gtcatcgcgc aacgatatgg
    21301 caccacggtt caggcgatcc tgaccgccaa tccgacgatc acgaacgcga acctgatctt
    21361 tgccgggcag acgatcttcg tgccgtttgg cgctcagccg ccgcagccga cgcctgtccc
    21421 gccgccaccg acgcctgtcc cgccggtgac gcctgcctgt cgctggaacc atctggtggt
    21481 gccgggcgac accatgctga agatcggccg cttgtatggc gtggatccgt tcgtcatcgc
    21541 gcgggccaac aatatcttca acctgaacct gatttttgcg ggttcgactt tatgtatccc
    21601 gtaaccgggc gctagaagcg ggcattgagc ctggctctag cccatgatag ctaatttttt
    21661 ccggggcggt ggcaacaccg ccccttttat ttgatttcgc cggacgggaa tttaacccgt
    21721 caccacattg accagccggc cgggcacaac gatcacatcg atgatcggcc gccggccaat
    21781 cgtcaaccga actttctcgt ttgccagggt ttcctcacgc atagtcgtct cgtcggcgcc
    21841 ggcggacatg gtcagtcggt cgcgcagctt gccgttcacc tgcacgatga tcgtgacttc
    21901 ctcgtcgcga gccagctctt cgtcataggc gggccagtct gcctggtgga ccgatttgcg
    21961 ctctcccagc acgtcacgcc acacttcctc ggttatgaac ggcgtgatag gagccagcag
    22021 cagcgtcagg gtgcggatgg ccgcgcgcca ttgatcgccg ggaattggcc acgagcgcgc
    22081 tgcatacaga tagttgacgt actccatcag tgcggccacg gccgtattga agcggaaccg
    22141 gctcatgtct ccggtcaggc gacggatgat cttgtggcgt gttcgctcga attcctcgtt
    22201 gattgcctga ccactattcg gatgcgacgc catctcgtga gccagtgtcc agtagcgctc
    22261 gatgaagcgc tggacgccga cgatgttccg attgtcccac agtgcgttgg cgtcaaacgg
    22321 ccccaggaac aggatgtagg cgcgcaggac gtcggtgcca tactcggcga ttacctcgtc
    22381 gggggtgatg acgttgcctt tcgatttgga catcttttgg ccgtcgtagg gcgacagcag
    22441 catgccctga ttgcgcagtt gggtgaacgg ttcgtcaaaa tcgatcagac cggcgtcgtg
    22501 gataactttc gtccagaagc gtgcgtagag cagatgcatg acggcatgtt ccgcgccgcc
    22561 gacgtaggta ttcaccggca gccaatgact tacggccgcc gggtcgaacg gcctgtcgtc
    22621 gatatgggga ttggcgaagc gcaggaagta ccacgatgaa caggcaaagc catccatcgt
    22681 gtccgtttcg cggcgagcgg ggccaccgca ttgcgggcag gtggtattga cccacgcttc
    22741 ggcgcgggcc aacggcgaac ggccgtcgtc cgagggagtg aaatcggtta tgtccggcag
    22801 caacaccggc agttcattct ccggaacggg caccgggcca catgcggagc aatggatgat
    22861 ggggatcggc gcgccccaat atcgctggcg gctgatcagc caatcgcgga aacgataggc
    22921 gacacgtggc ccgcctcgct cgcgggcggc cagttccgcg gcgatcttcg ccgacgctgc
    22981 ggccgaggac agtccgctat atgggccgga gttgatgacg atgccgggtt cttcgaacac
    23041 ggatgttacc tgatcgacga cggtcgatgg ttgactgtcg ctatctgtct gcggtcgacc
    23101 atcggtggtc ggccgtcgct cttccaccac cgctatgatc ggtagcctgt agcgccgggc
    23161 gaaggcgaag tcacgggcgt cgtgaccggg cacgcccatt actgcgcccg tgccgtagct
    23221 cgccagcacg taatcggcga cccagatcgg cacaggctcg cctgtcagcg ggtgggtggc
    23281 atagctgccg gtgaagacac cggtcttctc cttgtcggtc gtctgccgtt cgatctcgct
    23341 cattctcccg gcggcaaccc tgtaggcgtt cacttcggcc gcgcgatcgg gtatggtaac
    23401 gtgcgccgcc agcggatgct cgggagccag ggccaggaat gtgacgccaa acagcgtgtc
    23461 ggagcgtgtg gtgaagacgg ggatgggatg tccatcggcc atgaacacag cctcggctcc
    23521 ttcggagcgg ccgatccagt tgcgctgcat ctccttgatc cgatccggcc agtcgatcgt
    23581 atccaggccg ctcagcaggc gttcggcgta gtcggtgatg ctgaagtacc attgctccag
    23641 ttgcttgcgc tcgacgacgg aatcgcagcg ccagcagcgg ccgttctcca cctgttcatt
    23701 ggccagaata gtgcgacatt gcgggcacca ccactgactg cccgaagccc gataggccag
    23761 cccgcgcctg tgcagcagca gaaagagcca ttgtgtccag cggtagtagg cgggatcggt
    23821 ggagttgatc tcgcgcgacc agtcgtagga gcattcaacc agatccatct ggcggcggta
    23881 gttggccacg aagcgttggg tggtctccac ggggtggatg ccgtgcttga tggcatagtt
    23941 ttcggccggc agcccgaagg cgtcccaacc catcgggtgc agcacgttgt agccgcgcat
    24001 gcggtggaaa cgggcgctca cgcaagtagg gacatagttg cggcagtggc cgaccgacag
    24061 cccgtcgcct gaggggtagg ggaagaagtc caggatgtag tagttgggct tgtcggggtc
    24121 gtcgccggtt cgatacagat cggccgcccg ccagcgtggc cgccattttg attccagagc
    24181 gcggaagtca taaggtttac tgcgagattt tgattgggtt gtcatgacat cactctttag
    24241 aaagaagaat ccatagattt cacaaattgg cttctcgttg tcatctgcgg cctgctattt
    24301 gccccctgtt gccgaaaaac aaaaaggccg cagggagcac ctgcggcctt tgctggatga
    24361 attttgggat cgatcgttac gcaagacggg caggcagagg taaaccggcg actaaagcgc
    24421 caagtaataa gctctgcaac tgcgtggaat gcgatttggt gttcatgatc gattctggcg
    24481 acagagtagc ataggctgat ggcgatgtca atcgggtttg tcaaaaggtg ttattcttca
    24541 gcgaatctga caccccataa ggaaaaagcg aaaatgtcac cctaatggac aaggtgacat
    24601 tgaacaacaa ggaacagaag cgattgataa tgctcaacga ggcagggggc gggttgctcg
    24661 gtctgttgag catttggagc aaatggcaat cgccgcctgg tcgattgcct tccatcgctt
    24721 tctcgtcact ttcagtgtgg tatacttgcg cctcagaata ttcgctcaca ggcgttcgcg
    24781 gccgtttgcc cgcccgatta caaccggaaa cccctgaagt cctctcggtt ggcccaatac
    24841 ggagaagcag agcagccgca tcatacgccg gagcagggtc tggccggaaa cacttggaac
    24901 tattcgaatc cttactggaa cgcctgtcgc ttatcggcat tgtcgacatc atcgacatat
    24961 tgcttgtcac cctcatcatc tttggggtgc tgatgctgat ccgcggcaca cgagccgtgc
    25021 aggtgttgcg cggcttgctt gtgctgggcg cgattgtctt cctgctggct cagatattcg
    25081 aacttcgcgc gttcagttgg ctggttgaca acatgctgcc ggtcttgctc atcgccatac
    25141 cagtcatttt tcagccggaa ttgcggcgag cgctggagca actgggtctg gccggccgtt
    25201 atctgcggct gtttaagcgc gacgacacaa acccggttgt cgaggccctc aaggaggctg
    25261 cattgcgcct gtcgcagcgc cggcacggag cgctcgttgt ctttgagcag gacacggggc
    25321 tacaggaata catcgacact ggcgtgatcc tcgatgccga tccttcgccg gaactgttcc
    25381 tgacgatttt caacaagaat accgagctgc atgacggggc tattatcatc cgcgagaaca
    25441 agctcattgc cgcggcttgc gtgttgcccc tgtcgaccag cagcctgtcc gaccggcaga
    25501 tggggttgcg ccatcgcgcc gcgctgggta tcagcgaggt cagcgatgcc gtcgccgtgg
    25561 tcgtgtcgga agagacgggg caggtgtcca tcgcccacaa tggccgcatc ctgcgccgcc
    25621 aggacatcgc gcggctggat acgatcctgc acgcatttct gaccggtccc cgccagccgg
    25681 gctttcttcg gagggatagc agtgaaccga gcgggtgaga cactgaccaa cgtagccacg
    25741 ttcatcctgt ccctgatcct ggctatcttc atctggacgt cggcctcgca atcccaggat
    25801 ccgattcgga cgcggttcct cgaggtcccg gttcaatatg tcggcctgcc ggagaacacg
    25861 acattggtgg acgtggaccc gcggcagacc gttcagattc gactggaagg gccggacacc
    25921 atcctgcaga aatacacccc tgaggatttc tccgcgaccg tagatctgag ccaggtgcct
    25981 tcgggggagc gtgtgtcggt cgatatcaac gtcacgacca ccaaaaccgg tgcagagatc
    26041 tcgttcatca cgcctgagca ggtcgatgtt acgctggaag aacaagtgac ccgcgacatc
    26101 ccggtggaac tggacattcg cggctccgtg gcgcgcggcc attcgcaagg cgagccattg
    26161 attgagccgc cgacgatccg cgtgagtggg ccggccagcc aggttaatca actcaatttc
    26221 gccttggtga cgatatttct caacagcgcg gtggagaccc tggtagagac atcgacgccc
    26281 ttgttttatg atctggccgg gcgtgtgtcg agtgtcaacg cccttgcctt gagccacaac
    26341 acggtgtcgg tcacgatccc ggttgaggaa ctggcgggtt ttgccgacaa actggtgacg
    26401 gtcgtatgga cgggcgagcc ggcgccgggc tatcgattgc tcagcgtgac ggccgaccca
    26461 cccagcttgc tcgtcgaggg ccgcccggcg caagtcaatg cgctcaatac cgtgtccacc
    26521 gaggcaatcg acatcgatgg cctgacagaa tcgttccagc agacggccgt tctcgatttg
    26581 ccacctggaa tatcggtgga tgccgagcaa accgtgtcgg tcaagattga gatcgcaccg
    26641 atcctgacga ccagttcatt taatcgggtg ccggatccgc gcggcctgcg cgcaggttat
    26701 gaggtgaagg tggagccgga gcaggtgcgc gtggttctgt ttggccctct gccgttgctc
    26761 gattcgctgg ccgagaatga tgtccgcgtg atcctggatt tgtttgggct ggaacctgga
    26821 acctataccg tcaaacctga ggttgacatc cccgagcggg aaatcgagat caggtcgata
    26881 cagccatcgt cggtcacggt gaccatcagc cagaccgttc cgagcaccac gtcacttctc
    26941 gacgggcaac tcgttgcggc cgggcaagtt ccgttgtcgg ggtcgcctcc ggtgcggcct
    27001 gcccgcgtcc ccactaacta ttacccgatg tcgtggggcg ctattgcttc tcgcctgccc
    27061 gaattatacc ttgagagacg agggtttgaa tgaatcaaaa agcgttggtg gccctcgtcg
    27121 gccgcccgaa tgtgggtaag tctaccctgt ttaaccggat catcggccgt cgcctggctg
    27181 tggtgtccga cgtagccggc accactcgcg accgcctgta tgctgatggc gaatggggtg
    27241 gcgtggcctt cacggtggtc gatacgggcg gcattgaggt gaccgagggg cgcaataccg
    27301 cgccgttatc cgaggattcg gagcagttcc tgccgctcat ccggcagcag gcagtgatcg
    27361 ccatgcagga tgcggacgtg atcgttcagg tcgtggacgg ccagaccgga atcaccgccg
    27421 ccgatcgcga agtcgcttcc atcttgcgac aggccaagaa gccggtcatc gtcgccgcca
    27481 acaagctgga atcgagcaag ctgtgggaca cggcctacga gttctatgag ctggggctgg
    27541 gcgaggtgtt cgctgtgtcg gctctccatg gcgcgggcac cggcgatttg ctcgatgcca
    27601 tcgtcgaatc tattccgccg tttgatcccg atgacgatga agaggatacc tcgatcaaga
    27661 tcgctatttt ggggcggccg aatgtgggca agtcgaccct gctcaacaag atgatcggcg
    27721 aggagcgggt catcgtcagc cccatcgccg gaacaacgcg cgatgccatt gacgaaaaac
    27781 tgcgctggca tgggcaggac ttcacgatta tcgacacagc cggtatccgg cggcgcggca
    27841 agatcgatca ggggatcgag aaatacagcg tgctgcgagc catcaagacg ctgcgtcggg
    27901 cggatgtggc tttgttattg atcgacggcg aggaaggcgt gacggcccag gacgcccaca
    27961 tcggcggcat gctggtcgag gaaaacgtcg gcgtgatcct gatcgtcaac aaatgggatg
    28021 ccgtggaaaa agacacccac accatgcccg cttacgagac ggacgtgcgg cgggagctgg
    28081 ctttcctgtc gtatgcgccg ctgttgttta tctcggccga gacaggccaa cgcgtgaaca
    28141 agatcctcga gacggtgatg gcggtctatg aggcgcgcca aactcgcctc tccaccagcg
    28201 agatgaacga cctgatgcgc gatattgttg cccatcatcc gccgccgacg aagggcggca
    28261 cgcagctcaa gttctattac gccacgcagg tggccgtcgc cccgccgacc tttgtgttct
    28321 tcgtcaaccg gccggagatg ctccatttcg gctacgagcg ctacatggag aaccgcatcc
    28381 gcgagcagta cccgttcacc ggcacgccta tccggttgct gttccgcggc cggacggacg
    28441 agcgcaagcc ggggcaaccc aagtaggtcg acgggcgctt atcgctctac caacgagagg
    28501 agcgcctctt tcatctgacg gccgatggcc gggatgctgt tgcccgccag atagcgcgcg
    28561 tgcccggctg cggcgattcg ctcgcgcagg gccggatcgt cgcgcagttc gtgcagcc
//
LOCUS       DAOMQG010000007        28070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_738_length_28070_cov_11.399964, whole genome shotgun sequence.
ACCESSION   DAOMQG010000007 DAOMQG010000000
VERSION     DAOMQG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 28070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28070
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_738_length_28070_cov_11.399964"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLC06_01020"
     CDS             complement(<1..878)
                     /locus_tag="PLC06_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89054.1"
                     /translation="MNSELTATGDNWRERIAELQALLEELRPELIEAEAQLADRLAAI
                     SAFEYTVRARLEPLSRRLDELQAEIDTLRRELRRYQAGSLLWDEEANPKDSAGEEWRF
                     EESAASAGAYRYRRQTEAPRPAPGGKRLVLLKQLYRQLARRFHPDLVLDEADRAYRTD
                     MMMAINAAYAAGDLEALERLANEPDTISYEPRSPEELIAAFQREVDHCRRRLAEIAAE
                     MAMLENHDSARLMKRVEKAAAVGRDLLTELAADLRLRISEKMVERNVLETQLDEIEKE
                     GVEVSYDDLADIVYNLG"
     gene            complement(1037..2353)
                     /locus_tag="PLC06_01025"
     CDS             complement(1037..2353)
                     /locus_tag="PLC06_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO89055.1"
                     /translation="MSTLSGSNLQVESPIQPKHSTIGRVARRAGEGIVKLLIALLALL
                     LALPVILLPGTTAVPWFGWLALGIVDIALLVVLFTRAQTPVHVSLIFMAITGISILAI
                     ALSQWFAATPPIRDSTGRPIPGSIATLEAVELNGRRQWITIRGHDQTKPVLLFLAGGP
                     GGSQLAATRKSLGELEKHFVVVNWDQPGAGKSFHATDFATLTPEQYIADGHALTLYLR
                     QRFGQEKIYILGESWGSILGVWLVQRYPDHYQAMVGAAQMVDFLETDAYDYTLALRLA
                     FESGDFTKVGKLMRQGPPPYYGEGVAWKVAEYVLYLSQRMTSNPAVTGPGYDTLGDIA
                     SPEYGLYDKVNYFRGLITTMNAVWSQLWEVDLREQAPRLQVPIYFLEGRHDINAPPVL
                     VESYLQQLEAPHKEIIWFEHSGHSPWVDEAAKVVDIMVNTVLADTQ"
     gene            complement(2441..3010)
                     /locus_tag="PLC06_01030"
     CDS             complement(2441..3010)
                     /locus_tag="PLC06_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89056.1"
                     /translation="MTRSRKIVLILTAGAFITLMSICITGYISLRLANWGLSRITEDE
                     PTTVAEVGRSIAGYDVPVDFKEEYAIQVADFSLVAYQNTNQAGHIYLIQFPEAIEVDP
                     GALAQQLYEMADTNSWAELVVVYHIPCSIRGQAATLVISEGFSHDRQRYRSASAVFAG
                     NGGQALVNISGPADSWDQTMVDTFVQSLH"
     gene            complement(3007..3255)
                     /locus_tag="PLC06_01035"
     CDS             complement(3007..3255)
                     /locus_tag="PLC06_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89057.1"
                     /translation="MSSIEVVYEIRIQGILDAQRADWFPGMSIHSLSEGDTVLIGPVV
                     DQAALHSLLSRIRDLGVPLISVQRIEPPPTSSDMTGEP"
     assembly_gap    3325..3424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3425..4944)
                     /locus_tag="PLC06_01040"
     CDS             complement(<3425..4944)
                     /locus_tag="PLC06_01040"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRO89058.1"
                     /translation="MAADVLERAIQEVSTWSRGDVSRLTGWLDMLPDSILVTRPTLSL
                     HASRALYLSGQMERAEVLLHQAEQSLRDLDEGGLKAVAMVYRAAMSAMRGEDPAAAAT
                     MMRRLLDHPETMNIHTAARAADTLGLACELVGDVAAAEQAYRQAADLAQAAGVAYLAI
                     NARCECALVQITRGQLDSAEAMCRLALDSTSEETLPPTGLAWSILGEIARERNDPATA
                     EKLIPLGIEIARKGGIVDDLRHALLFLVRLRQAQEDWTAARTAWAEVDTLLRAYNIPR
                     LIDLSAAYRARLDLAQGYITSAGHWARHYQELRTSRADAYLREFEDLTLIRVLIAENK
                     ADEAMRWLRPLTTSAREAGRNRTVIEACILQAGILAGSDVPAACVALGDALALAADAG
                     FRQLFIDEGPSISPLLAKTRHIAPAFVDSLALPQSHAKADSLSDDTPTDLLTRQPLSP
                     RELDVLRLLAAGLSNQEIAATLVITVGTAKWHVHNIFEKLGARGRTQAVARAREQGL"
     gene            complement(4996..5988)
                     /locus_tag="PLC06_01045"
     CDS             complement(4996..5988)
                     /locus_tag="PLC06_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89059.1"
                     /translation="MTLISAPAGYGKTTLTVTWLQQHETQSVGPHFHVAWVSLDDDDD
                     NPNRFFACLAAAIQEAGVNWAPPNPHVWEFTDSPPVKTILTSLLNELNEFAGQASLIL
                     VLDDYHRIRSPVIHEALQFAVDHAPPNLHIILITREDPPLTLARWRARAQLTEIRAAD
                     LRFNPQEAAGFLAQTMALHLSADQIVALETRTEGWVAGLQLAALALKSTRPETGQSTE
                     AFITSFGGSHHYIIDYLMDEVLRRQDDTVRDFLRYTSVLERLCPSLCDAVSGHTDGRE
                     RLTQLEHANAFLIPLDNQREWYRYHHLMADSLQAGLDPQTRDIAHRRASQWHEA"
     gene            6291..7211
                     /locus_tag="PLC06_01050"
     CDS             6291..7211
                     /locus_tag="PLC06_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89060.1"
                     /translation="MIHTENLTKKFGDHVAVDRLNLDIAEGEVFGFLGPNGAGKTTTI
                     RMLTCLIAPTSGSVRVLDYEVGRDDQQIRRNVGILTETPGMYDRLTARRNLTIFAKLY
                     EVEHVDYQVDKYLKLLGLWERRDDPVGEFSKGMRQKLAIGRALLHEPKVLFLDEPTSA
                     LDPEAARLVHDFIGELKGKGRTIFLCTHNLDEADRLCDRVAVFKTHLRVLDTPGKLRR
                     QVYGRQVAFHLGGPAKEYLPAVQPFSYVKEATAADNKLVLSLDDPETHNPDIIRALVA
                     AGADIQFVGELRYSLEDVYLQLMKEDTTKA"
     gene            7233..8024
                     /locus_tag="PLC06_01055"
     CDS             7233..8024
                     /locus_tag="PLC06_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRO89061.1"
                     /translation="MDKMITIIRKEWAEVFKNKLIFSTVLFLPLILAIMPLGMLFAFG
                     SIDGMSAEMNDPEIMALAGEMCAGLNAMDCSYVYTLNMFVLMFMILPVAIPVTIAAYS
                     IVGEKTTRSLEPLLATPITTAELLGAKIIAAAVPAVIGTWIAFGLFLIGVKIMATPAV
                     FGEFFKVHWLLAIFFVSPLLAILSTCIAVIVSSRVTDPRVAEQLSAVVILPVIMLVIG
                     QSVGLILIDRRIMLWLGLAVLALDALLLWLAIRLFQRETILTRWK"
     gene            8107..8521
                     /gene="ssrA"
                     /locus_tag="PLC06_01060"
     tmRNA           8107..8521
                     /gene="ssrA"
                     /locus_tag="PLC06_01060"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9215..11815
                     /locus_tag="PLC06_01065"
     CDS             9215..11815
                     /locus_tag="PLC06_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89062.1"
                     /translation="MSKKVWALIALFVLVGGLMTACGGQQVVEVTRVVTETEVVEQEP
                     EVVEVEVTSAPVEVEVTRVVEVEVEPVNEEEATRKGGWLDTIVVIQEPDSNAAVARLE
                     AGDMQMYADDIGGEAALQAVASENIETRTQYGLFDELLFNIGTCSDATMLNPFANQTI
                     RQAMNFAMDRNYVAQELYQGLAVPKYTTIAEASADRARFAKEIRLEEAKWGYDLEKAR
                     GIITTEMEGMGATMDGGVWSFEGKPINIIFLIRNEDTRLLIGGYVATQLEELGFQVER
                     VERRSAELSPLWIESDPTACSWHLYTGAWSQTAIDRESNSSFEQYYTNRLLPWPLWAL
                     YAPVPELDEAAQIIYNGSFESLDQRNELFRTAIPLSNEYATRDWITSRTTVVPFRNEV
                     SVTTDLAGGVSGAPMWSKTIRYEDKVGGSMNIGLPSVFTEPWNPIGGSNWVFDMMVIR
                     GVGDAGLYPNPNTGLNFPNRIERAELTVREGYPMAVSSDWVTLAFEPEVTVPDDAWAG
                     WDAENQVFLTAAEVYTETQTALVKSTVYYPADLYTTVTWHDGSPISVADFIMGMITSF
                     DLGDPASANYDESLVPNREQFLAAFRGVRIASVDPLVIETWSEAGSLDAENAVTTWWP
                     NYDYGDAAWHNMALMLRGDANGGFAFTPDKATAAEIERINLVAGPSLEVLAGELISAT
                     AEAYVPYAPTLGQYVTAEDATARYTNLAEFARRYGHYYISTGSYFLSGVFPVEGQAVM
                     SNYAAHPDFANRFAGFSAPAFPVTEIDGPARVTIGQEASFDIFIDVFDGPYPAADIEA
                     VNYLVFGADGSLVAEGVAENVEDGVWNAVLGADVTGGLAEGANRLDVVVVSKLVAIPG
                     LSSYEFVTAP"
     assembly_gap    12048..12147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12148..12984
                     /locus_tag="PLC06_01070"
     CDS             <12148..12984
                     /locus_tag="PLC06_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89063.1"
                     /translation="KRFGLDRPFAIRSFEFLRDGMTLNLGYSQQLMSDSGSRLVRNIL
                     LERLPSTLILFGTANFILFFLALFAGLSLSRRYGSFADKAVLTLAPTSAAPAWFYGLF
                     LILIFAAWLKVLPFGGMVDAPPPDNALDYALSMAKHMILPIMAWLVSAIFLTAYNWRT
                     FFLIYSSEDYVEMAKAKGLSDSMIERRYVLRPTLPNIITSFALMLIGLWTGAIIFETV
                     FNWPGIGELYFRAIGVFDTPVIVANTVLYAYLLALTVFLLDFVYALVDPRVRVGGGGR
                     KQ"
     gene            12981..14342
                     /locus_tag="PLC06_01075"
     CDS             12981..14342
                     /locus_tag="PLC06_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89064.1"
                     /translation="MKGFIGVLRQMAHYPTAVIGMVVIALLLIVSIVTPIVIPYNEAV
                     RLWRGGENVWRFYPRNAAPAWFNWFRSTDQAGSIIMDSAEGDVESTIEQISADTREER
                     FSFEFDFPYDTFPSEMIMFASAKYASKQPFIEMTWITPDGREIRAGEFSSGPNTPIRF
                     DQDTRLQRRLERETGIKNVPVVKGLFDDPNQEGLQVLQGTYRLDATGLLFEPESTLDL
                     AFVSYGQVHGWAGTDHQRRDLSVALLWGTPIAMAFGLLAALLTTVTTMGIAAFGVWVG
                     GWVDAVIQRITEVNSIIPLLPILIMVGTLYSRSIWITLGLVIVLSIFGLQIKNYRAIF
                     MQVKESAYIDAARAYGAGDMRIIFRYLIPRILPLVIPAIVILVPSFVFLEASLAVLGL
                     GDPFIPTWGKLINDAQTQGALFNGYYYWILLPSVLLMIAGLAFAMVGFALDRVFNPRL
                     REV"
     gene            14348..15313
                     /locus_tag="PLC06_01080"
     CDS             14348..15313
                     /locus_tag="PLC06_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89065.1"
                     /translation="MEKKNELVLKVRNLRLYFNTRGGPVQAVDGVDFDLRKNEATVII
                     GESGCGKSSLAKAILRLLPRNAGLYDGEIWLHDVNIMNMDAETYRKEIRWKRISMVPQ
                     AAMNALNPVMKIGDQVAEPAMIHMNMNKEEATKSAVRMMEHVGVPVDFLHRYSFELSG
                     GMRQRSALAMALVTLPDVVILDEPTSALDVLTQASIMNLMKRMKTELETSFILITHDI
                     ATSSELADNVAVMYAGQVVEVSDARRFFTKPLHPYSHALMASVPRLRETIEPEFITGQ
                     PPSLLNPPTGCRFKDRCPKRFEKCDEEPPTVLVDGDRKVKCWLYA"
     gene            15353..16396
                     /locus_tag="PLC06_01085"
     CDS             15353..16396
                     /locus_tag="PLC06_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89066.1"
                     /translation="MTDTMTEISHTDHDNNGQALVQLKDLHVWYELKKFGFSHAGYVR
                     AVDNVSFDLNYGEAIACVGESGCGKSSLMKTILGLNIPTKGDVIFDGASLTHTKGEDL
                     RRYRSKIGYIQQDPFGALPPFMNVERILEEPLIINGVKDKEERRRRIEKILEEVKLTP
                     IAEVLPKFPHMLSGGQQQRVVIARAMIMEPKLLVADEPVSMLDASVRVEVLRLLRKLQ
                     ETHNLAVLYITHDLSTVRYFSEYIFVMYAGEIVEFARMDDLLDDSKHPYTHALLEASS
                     DPNADNALVQREIPKGEPPSLVNPPTGCRFHPRCPQFMPGICDVKAPPNIEVSPKHLV
                     ACWLYDENRGHAF"
     gene            16386..16607
                     /locus_tag="PLC06_01090"
     CDS             16386..16607
                     /locus_tag="PLC06_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89067.1"
                     /translation="MPFNRLIVLGFFMVLAGVILPFLIVMHVLPSTILLNFLAYGVSI
                     TGVFLGVIGVAMYVGEERRKSKDDWDEDR"
     gene            16771..17829
                     /locus_tag="PLC06_01095"
     CDS             16771..17829
                     /locus_tag="PLC06_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89068.1"
                     /translation="MGNNKNVILDIKNLKTQFFTEAGVVRAVDGIDFTVSRGEVVGLV
                     GESGCGKSVTSLSIMRLISQPGKIVDGQIIFDNQDLVELPESRMVEIRGNRISMIFQQ
                     PQSSLNPVFRIGDQLSEVLQIHQDVGKELGEKRAIELLALVGVPDPESRVKAFPHELS
                     GGMAQRVMIAMALACVPELLIADEPTTALDVTIQAQILDLMRNLRSKMDTAIILITHD
                     LGVVAEMCDRVNVMYAGRIVEQASVIDLFNSPMHPYTDALIGSTPVLGHADKPLTTIP
                     GSVPNLIKLPKGCKFSPRCAARVQYNLQICLEEEPELKLLSPGHTVRCWLYEDLPEMK
                     DAPAAQWKADRPELHKRG"
     gene            17846..18931
                     /locus_tag="PLC06_01100"
     CDS             17846..18931
                     /locus_tag="PLC06_01100"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRO89069.1"
                     /translation="MSQQETRATAPSRKKLIEVNNLVKYFPVRAGLMQRVKAWVKAVD
                     DVSFYINEGETLGLVGESGCGKTTVGRTILRLIPATSGEVLLDGKNVFDFDSSELKKM
                     RADMQIIFQDPYSSLDPRMPIGESIAEGLRVHTNMSGQERYDVVTEMLTRVGMRAEHA
                     RRYPHEFSGGQRQRIGIARALALRPKFIVCDEPVSALDVSIQAQVLNILSELQRDFGL
                     TYLFIAHNLSVVEHFSERVGVMYLGKMVEMAPRDLLYSDPLHPYTQALMSAIPVPDPT
                     YKRQRIILEGDVPSPLNPPSGCRFHTRCPIAFDLCSQEEPPFKDYGGGHFAACHWLEV
                     GGHGASKLTPDNIAANKGRDVAQKMIH"
     gene            complement(18963..19907)
                     /locus_tag="PLC06_01105"
     CDS             complement(18963..19907)
                     /locus_tag="PLC06_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HRO89070.1"
                     /translation="MRIGILSRNPGLYSTRRLAETARERGHRVEIIDTTTVAVHIGHN
                     NNSAAVPQRLVASQPGTAETTKLPMLDAVIPRIGTSVTFYGLAVVRQFETAGVLTTAS
                     SSAIARSRDKLQSLQLMSQVGLPIPRTAVVARPEALFSAIEAIGGLPAVVKLISGTQG
                     RGVFLAHQFATVAAILQKVAELNRQAIVQEFIAEADGRDMRLIVVGDRCVAAMQRQAP
                     AGEFRANLHRGGSASAFVPEPAIVAMAVAAARIHGLAVAGVDLVFSHRGPLLLEVNSS
                     PGLEGIEKATGIDVAGEIIGYVETALSQSKPARKSRSK"
     gene            20072..20848
                     /locus_tag="PLC06_01110"
     CDS             20072..20848
                     /locus_tag="PLC06_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRO89071.1"
                     /translation="MIIDTFVIVPTYNEADNLDELLEQLLALPTPIGAIVVDDNSPDG
                     TGDMAERWATENPGRVHVIHRPAKLGLGTAYIAGFKKAINDLAAERILTMDADFSHNP
                     RYIPDMIALSRTKHVVIGSRYVPGGGARNCTRKRIYLSRGANFVARTLLGLKARDATA
                     GFRLYRREVLQSIPLDEIFSSGYSFLVEMLFLCQRRGWQIGEVPIIFEDRRKGQTKIS
                     RQEILKAQYTVLRLFLRRLRGGEPRRPPITVSSAISNDKS"
     gene            20835..21740
                     /locus_tag="PLC06_01115"
     CDS             20835..21740
                     /locus_tag="PLC06_01115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89072.1"
                     /translation="MIKVNCNLCGRDEYEVRFPATTDGNALRVDAFRCTHSGYGHHPQ
                     IVECRHCGLIYANPRWSTDDVMSAYTAVQDETYVEERLGRELTFRHHLRHMERVIGPA
                     AGRALLDVGAYIGVFVEAAAAVGWQAQGVEPSEWAAAEARNRGLDVRVGTLDNVDLTE
                     ASFDVVTMWDVIEHLADPAAELDKARRLLRPGGWLVVHTMDIDAPVSRLAGSRWPWLM
                     DMHLYYFGKRTLRRMLSDHGYNVVWDGAQRRYLSLGYLASRVAGMNDALGRLSHALVG
                     GLGLNHSAIPVNFGDLRTYIGQRVR"
     gene            21816..22511
                     /locus_tag="PLC06_01120"
     CDS             21816..22511
                     /locus_tag="PLC06_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89073.1"
                     /translation="MDPIKLLIVDDHPLFRQGLVDVLESDPGLTVVAQAADGEVAVHH
                     AIEYQPDIVLMDVNLPNANGLRVTRRILNELPDTKVIILTGYDDAEQVFHALRIGAAA
                     YCSKDIPPEGLLATVYSVHQGGYVIHGQVMSYEEVLSWVQQRLGRYNYPIDEQSADTY
                     TPLSPREMEILDMVTRGSSNKEIALRLSISQQTVKNHMTAILRKLHVDDRTQAAVYAL
                     RHGWVRLDTSASS"
     gene            22561..22731
                     /locus_tag="PLC06_01125"
     CDS             22561..22731
                     /locus_tag="PLC06_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRO89074.1"
                     /translation="MRFFRSEDGQGLVEYALVLVLVAVVVIAILALLGPYIGDWLNNI
                     AGWFSQLLGNGG"
     gene            23335..23832
                     /locus_tag="PLC06_01130"
     CDS             23335..23832
                     /locus_tag="PLC06_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRO89075.1"
                     /translation="MPLRQMRAPLRYQEPTSDLIHRFKYEGYSALGRPLAHFLIAGWP
                     DWEQNPDLIVPVPLHPKRRRWRGYNQSELLARPLGSAKSIAVCASALRRTRDTIPQVG
                     LGPDERQSNVRGAFEADATVVDGRHVLLVDDVLTTGATMSAAAEALMAAGAVSVSAYC
                     LARVS"
     gene            23948..24505
                     /gene="raiA"
                     /locus_tag="PLC06_01135"
     CDS             23948..24505
                     /gene="raiA"
                     /locus_tag="PLC06_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRO89076.1"
                     /translation="MELQIFSTNMEVTPRLQNYVEKKTARLDRYMPTLTTIQVDLAME
                     NTRSAVQRHVAEITIRDERGTILRAEERNSDMFAAVDAVIDKLYRQIERYRGKQKRKY
                     RGKAGEVDMGEPLPIAEELEEEDRAIVRQKRFALHPMSAEEAIDQMELLGHDFYVFFN
                     SEDSAVNVIYRRRDGTFGLLQPEMG"
     gene            24520..24999
                     /locus_tag="PLC06_01140"
     CDS             24520..24999
                     /locus_tag="PLC06_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRO89077.1"
                     /translation="MANILIICTANICRSPVAAALLCDRLRRQGLADWTVRSAGTWAM
                     EPRGASRYSIDVVRREGLDLSRHRATMVDEPQLANADLVLTMEVGHAEALRAEFPQQA
                     HKVFMLTEMIGHTYNIPDPYGGPYEEYERMYEGLTSVIDQGLDRIIELARENTNGRG"
     gene            complement(25092..25613)
                     /locus_tag="PLC06_01145"
     CDS             complement(25092..25613)
                     /locus_tag="PLC06_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRO89078.1"
                     /translation="MSSNGLLQEPVLILNVTFEPIHVCSTKRALALVLSGKAEIVING
                     RGTIHSAASEFEIPSVIKLSHMIRRPRPRIALSKREILRRDDYTCQYCGRKLRTLTLD
                     HVIPRRQGGGHTWENLVAACAPCNRRKGGKLAAEMNMTLHRMPFEPNPSAAYRFGSLL
                     EQRQEWAQFIEGW"
     gene            25691..26878
                     /locus_tag="PLC06_01150"
     CDS             25691..26878
                     /locus_tag="PLC06_01150"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRO89079.1"
                     /translation="MQQDGRTAWLRDEIENLKGAGLYNNIRTIDSPMDAWVTIDGRRL
                     LNFCANNYLGLANHPRIRQAAQQGIEAYGVGPGAVRSIAGTTTLHVDLEQELATFKRA
                     EACITFQSGFTANLATIPALVGPGDLVFSDELNHASIIDGCRLSRAEVIRYAHNDVDD
                     LRTKIAGREDYKRRLIITDGVFSMDGDIAPLDKITEIAEEFGIMLMVDDAHGEGVLGH
                     GGRGIVDHFGLHGRVDVEVGTLSKAFGVVGGLVAGKAIIIEWLRQRGRPFLFSSAMTA
                     PDAAACIEAVKILSESTELIDRLWSNAKMFRDEMQAMGFNTGFSQTPIVPVMLGEATL
                     AQQFSRRLFEEGVFAMSIAFPTVAKGKARIRVMNSAAHNHSDIEIALETFNRVGRELG
                     VIK"
     gene            complement(26868..>28070)
                     /locus_tag="PLC06_01155"
     CDS             complement(26868..>28070)
                     /locus_tag="PLC06_01155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO89080.1"
                     /translation="IPNLVKSGFNGDIISTFATRDLCNIMLRDSARIQESDIEYLNKK
                     RERDNLPPLEPIYTMNDALDSLKLFIGIGYERPYHVAPGIALTLYDAGHVLGSAIVAL
                     DIEDKDGGPTQRLVFSGDLGRANRPILKDPVFLDEADILIMESTYGDREHPDDEDTDH
                     KLEDVIRDTVRQGGKVIVPAFAIGRTQELVYRLNNLVEAGDLPRDLAVYVDSPLAIDA
                     TSIFRAHPEAYDEEVAEVMMTDRDHDPFGFHLMHYTRSTQESKALNFVKTPAVIISAS
                     GMAEAGRILHHLKNNVEDSRNTILITGFQAPNTLGRRIVEKQKRIKIFGEDYDLRAQV
                     ESLSGMSGHADRSELIAWAGHFKRPPRQTFLVHGEPDAAFELAERLRAQLGFPQVTVP
                     ELGERFTL"
BASE COUNT         5475 a         7920 c         8447 g         6028 t
ORIGIN      
        1 cccaggttgt agacgatgtc ggccagatcg tcgtagctga cctctacgcc ttccttttct
       61 atctcatcca gttgggtttc cagcacgttg cgctcgacca tcttttccga gatgcgcagg
      121 cgcagatcgg cggccagttc ggtcaacagg tctcgcccga cggcggccgc tttttcgacg
      181 cgcttcatca atcgcgcgct atcgtggttt tccagcatcg ccatctcggc cgcaatttca
      241 gccaggcggc gacgacaatg atccacctcg cgctgaaacg cggcgatcag ctcttcgggc
      301 gagcgcggct cgtaggagat ggtgtctggc tcattggcca accgctccag ggcttcgagg
      361 tcgcctgcgg cgtaggcggc attgatggcc atcatcatat ccgttcggta ggcgcgatcc
      421 gcttcgtcga ggaccagatc ggggtggaag cgacgggcca gctgccgata gagctgcttc
      481 agcaagacca gccgcttgcc tcccggtgca ggacggggag cctcggtctg ccggcggtag
      541 cgataggcgc cggctgaagc ggcgctctcc tcgaaacgcc attcctctcc ggcgctatcc
      601 ttggggttcg cctcctcgtc ccaaagcaaa ctcccggcct gatagcggcg cagttcccgg
      661 cgcagcgtgt ctatctcggc ttgaagttcg tcgaggcggc ggctcaacgg ttccaggcga
      721 gcgcggactg tgtattcgaa ggcgctgatg gccgccagac gatcggccag ttgggcctcg
      781 gcttcgatga gttcggggcg tagttcttca agcaaggcct gaagttcggc tattcgctcg
      841 cgccaattat ctccggttgc ggtcagttcg ctgttcatcc gtcactctgt gccgagttgg
      901 ggcatcatgt tccatgatag cacaattgcc gcgagccaat ggcaatatgt ggggcgaaga
      961 taaagatgac ccaaaatacg aacggcgcat cgtgatctga cgcgccgttc gtatgagtga
     1021 tgctatgcag ctgtcgttat tgtgtgtcgg ccagcactgt attgaccatg atgtcgacta
     1081 ccttggcggc ctcatcgacc cacgggctat ggccggaatg ttcgaaccag atgatctcct
     1141 tgtgcggggc ttccagttgc tggagatagc tctccaccag aactggtggt gcgttgatgt
     1201 catgacgccc ttccaaaaag tagattggca cctgcaaccg cggggcctgc tcgcgcagat
     1261 ccacctccca gagttgggac cagaccgcgt tcatggtggt gatcaggcct cgaaagtagt
     1321 tgaccttgtc atacaggcca tattcaggcg aggcaatgtc ccccagcgtg tcgtagcccg
     1381 ggccggtgac agccggattg ctcgtcatgc gttggctcag atagagcacg tattcggcga
     1441 ccttccaggc gacaccctcc ccgtaataag gaggtggccc ctgacgcatc agtttgccga
     1501 ctttggtgaa atcgccgctt tcgaaggcca gtcgcagggc gagcgtgtaa tcataggcgt
     1561 cggtttccag aaaatccacc atctgggccg cgccgaccat ggcttgatag tgatcgggat
     1621 agcgctgtac cagccagacc ccgagaatgc tgccccacga ttcgcccaga atgtagatct
     1681 tctcctgacc gaagcgttgc cgcagataga gcgtcagggc gtgcccgtcg gcgatgtact
     1741 gttcgggcgt gagggtggcg aaatccgtgg cgtggaatga tttgcccgcg ccaggttgat
     1801 cccagttgac caccacgaaa tgcttctcca gctcgccaag ggatttgcgg gtggcggcca
     1861 actggctgcc gcccggcccg ccggccagaa acaagagaac cggcttcgtc tgatcgtggc
     1921 cgcggatggt gatccattgc cggcggccgt tcagttccac ggcttccaat gtggcgatgc
     1981 tgcccggaat gggtcgacct gtggaatcgc gaatcggggg tgtggcggcg aaccactgcg
     2041 acaacgcgat cgccagaatc gagatgccgg tgatggccat gaagatgagc gatacgtgga
     2101 ccggcgtttg ggcgcgggtg aagaggacga ccagcaatgc gatatcgacg attcccaacg
     2161 ccagccagcc gaaccacggc actgcggtcg ttcccggcag caggatgacc ggcagggcaa
     2221 gcaatagcgc cagcagcgcg atgagaagct taacgatgcc ctctcctgcc cggcgcgcta
     2281 cccgcccgat agttgagtgt ttgggttgaa ttggtgattc aacctgcaag ttcgaaccgg
     2341 ataatgtgct catgagaaac tcctgtgttt ggccggtctt tgttcatccg gcccatgcaa
     2401 gaaacgtgtg cggattaaca atgtcgattt ccctgctttg ttaatgaagc gactgcacaa
     2461 aggtatctac catggtctgg tcccacgagt cggccgggcc gctgatgttg accagcgctt
     2521 gcccaccgtt gcccgcgaag acggcgctgg cagagcgata gcgctgccgg tcgtggctga
     2581 acccttcact gatgaccaac gtggccgcct gaccgcggat cgaacagggg atgtggtaga
     2641 cgacgaccag ttcagcccat gagttcgtgt cggccatctc atacaattgt tgcgccaatg
     2701 cgccgggatc gacttcgatg gcctcgggga actggatcag atagatatgg ccagcctgat
     2761 tggtattttg ataggcaacc agcgagaagt cggccacctg tatggcgtat tcttccttga
     2821 agtcgacggg tacatcgtag ccggcaattg aacggccgac ttcggcgact gtggtcggtt
     2881 cgtcctcggt gatgcggctc aggccccaat tggccagtcg caggctgata tagccggtga
     2941 tgcagatgct catcagggtg atgaatgcgc cggcggtcag gataaggact atcttgcgtg
     3001 aacgggtcat ggctctcctg tcatgtctga ggatgtgggt ggcggctcta tccgctgtac
     3061 cgagatgagc ggcacgccca gatcgcgtat tcgactcaat aagctgtgca atgccgcctg
     3121 gtcgaccacc gggccgatca gaacggtatc gccctcggat agggagtgga tgctcatgcc
     3181 cgggaaccag tcggcccgct gagcgtccaa tatgccctgg atgcgtatct cataaacgac
     3241 ttcaattgaa gacataggat gctccattac ttgtgacgtt ctattggcag gataaccggg
     3301 agcggatagg gttgtcgcct atcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnagaccc tgctcgcggg cgcgagcgac ggcctgggtt cgcccgcggg cgcctagctt
     3481 ctcgaagatg ttgtggacgt gccatttggc cgtgccgacg gtgatgacca gcgtggcggc
     3541 gatctcctga ttcgacaaac cggcggccag caaacgaagc acatcaagct cgcgcgggct
     3601 gagtggctgt cttgtcaaca ggtcagttgg tgtgtcatcg gagaggctat cggctttggc
     3661 atgcgactga ggaagggcca gtgaatcgac aaatgccggc gcgatgtggc gagtctttgc
     3721 cagcaggggc gagatggacg ggccttcatc gatgaacaat tgccggaacc ccgcatcggc
     3781 agcgagcgcg agcgcatcgc caagcgcgac acaagccgcc ggaacgtcgc ttcccgccag
     3841 tattccggct tgcaggatgc acgcctcaat gacggtccga ttgcgtccgg cttcgcgcgc
     3901 agaggttgtc aacggacgca accatcgcat ggcttcgtcg gccttgttct cggcgatcag
     3961 aacgcgaata agggtaaggt cttcaaactc gcgcaggtaa gcgtctgccc gggatgtgcg
     4021 cagctcctga tagtggcgcg cccaatgacc tgctgaggta atgtacccct gtgccagatc
     4081 gaggcgggcg cgatatgctg ccgatagatc gatcagacgg ggtatgttgt aggctctcag
     4141 gagtgtgtcg acttcggccc aggctgtccg ggcggccgtc caatcctctt gcgcttgcct
     4201 aagacgaacc agaaaaagca aggcgtggcg cagatcgtcg acgatgcctc ccttccgcgc
     4261 gatctcgatc ccgaggggaa tgagtttttc agcggtggcc ggatcattcc gctcgcgggc
     4321 aatctcgccg aggatgctcc atgccagccc cgtgggggga agggtttctt cgctggttga
     4381 atcaagcgcc agccgacaca tggcctcggc cgaatcgagt tgcccgcgtg tgatttgcac
     4441 gagcgcacat tcgcaacggg cattgatcgc cagataggcc acaccggccg cctgggccag
     4501 atcggcggcc tgccggtagg cttgttcggc cgcagccacg tcaccgacca gctcgcaagc
     4561 caaacccagc gtatcggcgg cacgggcggc cgtgtggatg ttcatcgttt caggatggtc
     4621 gagtaagcgg cgcatcatgg tagccgccgc ggccgggtct tcgccgcgca tggcgctcat
     4681 ggcggcgcga tagaccattg ccacggcctt taatccgcct tcgtcgagat cgcgtaacga
     4741 ctgttcggcc tgatgcagca agacctcggc gcgctccatc tgcccggaca ggtacagggc
     4801 gcgagaggca tggaggctta aggttggtct tgtgaccagg atagaatcgg gcagcatatc
     4861 cagccagcct gtcagtcggg atacatcgcc gcgcgaccac gtggagactt cctgaattgc
     4921 tctttccagc acgtcggccg ccaaaatcgg atcgccggcg gcgaaggcgt ggcgaacggc
     4981 ttcagcatac agaccctagg cttcatgcca ttgggacgcg cgccggtggg cgatatcgcg
     5041 cgtctgtgga tccagccccg cttgcaggga atcagccatc aggtgatggt agcgatacca
     5101 ctctcgttga ttatccagtg gaatcagaaa ggcgttggcg tgctccagtt gtgtgagtcg
     5161 ctcgcggccg tccgtgtggc cgctcacggc atcgcacagg gaagggcaga ggcgttccag
     5221 aacagaggtg tagcgcaaaa aatcccgcac ggtgtcgtcc tgacggcgca acacttcgtc
     5281 catcagatag tcgatgatgt aatgatggct gccgccgaag gaagtgatga atgcctcggt
     5341 cgactggcct gtttcaggcc gggttgattt cagcgccagc gccgccaact gtaagcccgc
     5401 aacccatccc tctgtgcgag tttccagcgc cactatttgg tcggccgaca ggtgcagcgc
     5461 catcgtctgg gcgagaaagc cggccgcttc ctgggggttg aaacgcaggt cggccgcgcg
     5521 gatttccgtc aattgcgccc gtgctcgcca acgggccagc gtcaggggtg gatcttcacg
     5581 cgtgatcagg atgatgtgga gattcggcgg ggcgtgatcc acggcgaact ggagcgcctc
     5641 gtggatgacg ggcgagcgga tccggtggta atcatcaagc accagaatca acgatgcctg
     5701 cccggcgaat tcgttcaatt catttagcag ggaggtgagg atcgttttga cgggcggcga
     5761 atcggtgaat tcccatacgt gcgggttagg cggtgcccaa ttaactcccg cttcctggat
     5821 cgcggccgcc aggcaggcga agaacctgtt tgggttatcg tcgtcatcat ccagcgagac
     5881 ccaggccaca tggaaatggg ggccgaccga ttgtgtctcg tgttgttgca gccaggtgac
     5941 ggtaagggtc gtcttgccat agcccgcggg ggcggaaatc agcgtcaagg ggcgtctttg
     6001 ccgtaaccct tcttcaagct gcgccagtag ccgcggccgc gctaccagat cggcccgcaa
     6061 ggaaggagca aagaatttcg tcctcagtag gtcccgcttg atcgtcattg gagttgaatc
     6121 cgaccccgct cggactgtcg ggtgctcatc ctgagtatac cattatcaca ggacgaggtg
     6181 ctgtgtatcg ttgtcaaaat gacaaggtct ctcgctcttg tcaggtataa acaacgcttg
     6241 acgggccggg cggctggcgt taaagtgatc tataatgctg gagtttcgtt atgatccaca
     6301 cggaaaatct cacaaagaaa ttcggcgacc atgtcgccgt cgatcgtttg aatctcgaca
     6361 tcgccgaagg cgaggtgttc ggttttctgg ggccgaacgg cgcggggaag accacgacga
     6421 tccgcatgtt gacctgcctt atcgcgccca cgtcgggcag cgtgcgcgtc ctcgattatg
     6481 aggtcggccg cgacgaccag cagatccggc gcaatgtcgg catcctgacc gagacgcccg
     6541 gcatgtacga tcggctgacg gcgcgtcgca acctgacgat cttcgccaaa ctttatgaag
     6601 tcgagcatgt ggattatcag gtggataaat atctcaagct gctcggtctc tgggagcggc
     6661 gggacgatcc ggtgggcgag ttcagcaagg gcatgcggca gaagttggct atcggccggg
     6721 cgctgctcca cgagccgaaa gtactcttcc ttgatgagcc gacgtccgcc ctcgacccgg
     6781 aggcggcccg cctggttcat gacttcatcg gcgagctaaa gggcaaggga cgcaccatct
     6841 ttctgtgcac ccacaacctg gatgaggccg accggctgtg tgaccgggtg gccgtgttca
     6901 agacccatct gcgcgtgctc gatacgcccg gcaagctgcg gcggcaggtt tatgggcggc
     6961 aggttgcttt ccatctgggc gggccggcca aggagtatct gccggctgtg cagccctttt
     7021 cctatgtaaa ggaagcgacg gcggccgata acaagctcgt cctgtcgctg gacgatcccg
     7081 agacccacaa tcccgacatt atccgggcgc tcgtagccgc gggggccgat atccagttcg
     7141 tgggtgaatt gcgctattcg ctggaagatg tgtacctgca attgatgaaa gaggacacaa
     7201 ccaaagcctg atggggcggg gcggataatc tcatggataa aatgatcacc atcattcgca
     7261 aggaatgggc cgaggttttc aagaacaagc tcatcttttc cacggtcctg tttctaccgc
     7321 tcattttggc catcatgccg ttgggcatgt tgtttgcctt cggcagcatc gacgggatga
     7381 gcgccgagat gaacgacccg gagatcatgg cgctggccgg ggagatgtgc gcgggcctca
     7441 atgcgatgga ttgctcgtat gtctacaccc tgaacatgtt tgtgttgatg ttcatgatcc
     7501 tgccggtggc cattccggtg accatcgccg cctacagcat cgtgggggag aagacgaccc
     7561 gcagcctgga gcctctgctg gcgacgccga tcacaaccgc cgagttgctc ggggcgaaga
     7621 tcatcgccgc cgccgtgccc gccgtcatcg gcacctggat cgccttcggc ttgtttctta
     7681 tcggcgtgaa gatcatggcg acgccagccg tcttcggcga gttcttcaag gttcactggc
     7741 tattggccat cttttttgtg tcgcctctgc tggcgatatt gtctacctgc atcgccgtca
     7801 tcgtgtcctc gcgcgtgacc gacccgcggg tggcggagca gctttcggcc gtggtcattc
     7861 tgccggttat catgctggtc atcggccaat cggtcgggct gatcctcatc gatcgccgga
     7921 tcatgctctg gctggggctg gccgtgctgg ctctcgatgc cctgttgctc tggctggcga
     7981 tccggctctt ccagcgagag acgattttga cccggtggaa gtaggggatg ccgggtgtcg
     8041 ccgccggatt tttacttatt tcaaatagac ggccgcggct gatgatgcta tactgatgtt
     8101 atgttcgggg atgactggct tcgacgggga aggctggccc tggattgcaa gccgagcacg
     8161 ctttacacct cgtaaaactg gaagcacacg cataagtgcc aaccgcacca atgcatacag
     8221 cgctctcccc atggctgcct aaagcggcat aagggaaagc cagatgttcc cctaaacaag
     8281 gaacccgttc gctatcgctc tctcgtcgac tgggctttgg cgggcgacac taatgtcgac
     8341 gtgctctgcg cagatgccga tatgcgcggc tagagacgcc ctttcggggg taatcggata
     8401 ggcccggctg acccggttga tcggggcgcc cggcgccgaa acctaaatag atcgactaag
     8461 cttgtagaca tctggaggtc gaatttttcg gacgcgggtt cgattcccgc catctccacc
     8521 aatcagggtt ttcccggcag caggccacca tttcggtggc ctgttttgtt ttgtcgcccg
     8581 aacggccgcc cctgttgcac gtaaacagat gaggggtagg ggtgaggcag cggcgatttc
     8641 atcgccgttt tgcagaacca ccctcattcg ccgctgcctc gccctcttcg caggagggtc
     8701 aggcaaccgg gaagggaggc cgcgcgtttc catggagatt attgcccggt tgcctgaccc
     8761 ctactgcatc cttgattaat taaatatcca aaaggggcgc gatcccgctg aacccggccc
     8821 ccataggcca cctttcgtca cctaaatgtt actaatttga cagcaaaatt gtgttgaact
     8881 atactgtaac gcaggatatc ttgtagcgct taaaacacat gagactgtct gtcccgattc
     8941 cggttgaaaa caaaaccctg tcccgacaac gcagcgttgt ccttgccgat ccggcatggg
     9001 cgatggctgc gtttttgttt tcctggcaca acagttgagt tacttgagga ggtgatgccg
     9061 taacccaaga gggatatcga aatgcctaaa cgtattgaga taatcacgct tatgagcaca
     9121 gttggatgaa cgtcgacata ggccaggccg atcaaatctg tcgttcggcc caggccacga
     9181 acctgacaat ctcgttagag gaggacaacc tataatgtcg aagaaagtgt gggcgcttat
     9241 tgcgctgttt gttttggtag gtgggcttat gactgcctgc ggcggccagc aggtcgtcga
     9301 ggttacccgt gtcgtcaccg agacggaagt tgttgagcaa gagcccgaag tcgtcgaagt
     9361 tgaggtgacc agcgcgcccg tcgaggtcga agtcacccgc gtcgtcgaag ttgaagttga
     9421 gcccgtgaac gaggaagagg ctacccgcaa gggcggttgg ctcgacacca tcgtcgttat
     9481 tcaggaaccg gactccaacg cggccgtggc ccgtctggaa gccggcgaca tgcaaatgta
     9541 cgctgacgac atcggcggcg aagcggccct gcaggccgtc gcttccgaga atatcgagac
     9601 ccgcacgcag tacggtctct ttgatgagct gctgttcaat atcggcacct gctcggacgc
     9661 cacgatgctg aacccgtttg cgaaccagac gattcgccag gcgatgaact tcgccatgga
     9721 tcgcaactac gtcgctcagg aactgtatca gggtctggcc gttcccaagt acacgacgat
     9781 tgccgaagcc agcgccgacc gcgcccgctt cgccaaggaa atccgcctgg aagaagccaa
     9841 gtggggttat gaccttgaga aggctcgtgg gatcatcacg accgagatgg aaggcatggg
     9901 cgccacgatg gacggtggcg tttggtcctt cgaaggcaag cccatcaaca tcatcttcct
     9961 gatccgtaac gaggacaccc gcctgctgat cggtggctat gttgctaccc agctcgagga
    10021 attgggcttc caggtcgagc gcgtcgagcg ccgttcggcc gagcttagcc cgctgtggat
    10081 tgaaagcgat ccgaccgctt gctcgtggca cctctacacc ggcgcctgga gccagacggc
    10141 catcgaccgc gagtccaact cttccttcga gcagtactac accaaccgtc tgctgccgtg
    10201 gccgctgtgg gcactctatg cgccggttcc tgagctggac gaagccgcgc agattatcta
    10261 taacggcagc ttcgaatcgc tcgatcagcg caatgagctg ttccgcacgg cgatcccgtt
    10321 gagcaacgaa tacgcgaccc gcgactggat caccagccgt accaccgtcg tgccgttccg
    10381 caatgaagta tcggtgacga ccgacctggc cggcggcgtc agcggcgccc cgatgtggtc
    10441 caagacgatt cgctacgaag acaaggttgg cggctccatg aacatcggcc tgcccagcgt
    10501 cttcaccgag ccctggaacc ccatcggcgg ctccaactgg gtcttcgaca tgatggtcat
    10561 ccgcggtgtt ggtgatgcgg gcctctaccc gaatccgaac accggcttga acttcccgaa
    10621 ccgcatcgag cgggccgaac tgaccgtccg cgagggctac ccgatggctg tgtcctccga
    10681 ctgggtcacg ctcgccttcg agcctgaagt caccgtcccc gacgacgcgt gggccggctg
    10741 ggatgccgag aaccaggtct tcctgaccgc tgccgaggtt tacaccgaga cgcagaccgc
    10801 tctggtcaag tcgaccgtct actacccggc cgacctgtac acgaccgtga cctggcatga
    10861 cggcagcccc atcagcgtgg ctgacttcat catgggcatg atcacctcgt tcgacctggg
    10921 cgatcccgcc agcgcgaact acgatgagtc gctggttccg aatcgcgagc agttcctggc
    10981 cgccttccgc ggcgtccgca tcgcttccgt cgatccgctg gtcattgaga cctggagcga
    11041 ggctggcagc ctggacgccg agaacgccgt cacgacctgg tggccgaact acgactatgg
    11101 cgacgctgcc tggcacaaca tggccctcat gctgcgtggt gatgccaacg gtggcttcgc
    11161 cttcacgccc gacaaggcca cggcggccga gatcgagcgc atcaacctgg ttgccggccc
    11221 gtccctcgag gtcctggctg gtgaactgat ctccgctacg gccgaggcct acgttcccta
    11281 cgccccgaca ctgggtcagt acgtgactgc tgaggatgcg accgcgcgtt acaccaacct
    11341 ggctgagttc gctcgccgct acggtcacta ctacatcagc accggctcct acttcctgtc
    11401 cggtgtcttc cccgtcgagg gtcaggccgt gatgtccaac tacgcggccc acccggactt
    11461 cgccaaccgc ttcgccggct tctctgctcc cgccttcccg gtgacggaga ttgacggacc
    11521 cgcgcgcgtg accatcgggc aagaagccag cttcgacatc ttcatcgatg tcttcgacgg
    11581 tccttacccg gccgccgata tcgaggcggt taactacctg gtgttcggcg cggacggatc
    11641 gctggttgcc gaaggcgtgg cggagaacgt cgaggatggc gtctggaacg ctgtcctggg
    11701 cgccgatgtc accggcggcc tggccgaagg cgcgaaccgc ctggatgtcg tcgtcgtctc
    11761 caagctggta gccatccccg gcctgtccag ctacgagttc gtgaccgccc cgtagttgac
    11821 tttctttctt aatcggtagc acagacgatc ggatgtgcta aaatgtagat aaatgtagag
    11881 gaggtgactt tcgagtcacc tcctcctact gataaccctg ttcttctcgc gaaatttgag
    11941 gaggtgtgac catggcgaca gacgttatca ccggcggcct ggatttcaac gccgaagtag
    12001 atgtcgaatc cattgaagcc tcggcacgca aagcccggat gcagagcnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa cggttcggcc tggaccgacc gttcgccatt
    12181 cgaagctttg agtttctgag agacgggatg accctgaatc tcggttactc ccagcagctg
    12241 atgagtgaca gcggctcgcg actggtgaga aatattcttc tggagcgatt gccgtccact
    12301 ttaatcctct ttggaaccgc aaacttcatt ttgtttttcc ttgcattgtt cgccgggttg
    12361 agtctgtccc ggcgttacgg cagttttgcc gacaaggcgg tgctgacgct ggcgccgact
    12421 tcggccgcgc cggcatggtt ctatgggctt ttcctgatcc tgatctttgc cgcgtggctc
    12481 aaggtattgc cctttggcgg gatggtggat gcgccaccgc ccgataacgc tctggactac
    12541 gcgcttagta tggcgaagca tatgattctg cccattatgg cctggctcgt ttccgccatt
    12601 ttcctgacgg cctataactg gcggacgttc ttcctgattt attccagcga agattacgtg
    12661 gagatggcca aggccaaagg gctttccgac tcgatgatcg agcgacgtta tgtcctgcgc
    12721 ccgacactgc ccaatatcat caccagtttt gccttgatgc tcatcggcct ctggaccggg
    12781 gccattatct ttgagacggt ctttaactgg cccggaatcg gcgagctata ttttcgggcg
    12841 atcggtgtat tcgacacccc ggtgatcgtc gccaatacgg tgctgtacgc ctatttgttg
    12901 gccctgactg ttttcttgct ggactttgtc tatgctctgg tcgatccgcg ggtgagggtc
    12961 ggaggtggag gtcggaaaca atgaaaggat tcataggtgt tctgcggcag atggcccatt
    13021 atccaacggc cgttatcggc atggtcgtta ttgccttgct gttgatcgtc tctatcgtga
    13081 cccccatcgt cataccctat aatgaagcgg tgaggctgtg gcgcggcggc gagaacgtct
    13141 ggcgttttta cccccgcaac gccgccccgg cgtggtttaa ctggttccgg agcacggacc
    13201 aggccggatc gatcattatg gatagtgccg aaggagacgt tgaatccacg atcgaacaga
    13261 tttcggccga cacgcgcgag gaacgtttct cgttcgagtt tgacttcccc tatgacacct
    13321 tcccctcgga gatgatcatg tttgcctcgg cgaaatatgc cagcaaacag ccttttatcg
    13381 agatgacctg gataacgccc gacggccggg agattcgtgc cggcgagttc tcgtccgggc
    13441 ctaacacgcc tatacgtttc gaccaggaca ctcgcttgca gcgtcgcctg gagcgggaaa
    13501 ccgggatcaa gaacgtcccg gtggtcaaag gcctttttga tgatcccaat caggaagggt
    13561 tgcaggtgct ccaggggact taccggctgg acgccacagg cctgttgttt gaaccagagt
    13621 cgacactcga tctggcattt gtcagctatg ggcaggtcca cggctgggcc ggaaccgacc
    13681 accagcgacg cgatctctcc gtggctctgc tgtgggggac gccgatcgcc atggcctttg
    13741 gtttgctggc cgccctgttg acgaccgtca ccaccatggg gatcgccgcc ttcggcgtct
    13801 gggttggtgg ctgggttgac gcggtcatcc agcggatcac ggaggtgaac tccatcatcc
    13861 cgctcttgcc tatcctgatc atggtgggca cgctctatag ccggagtatc tggatcacgt
    13921 tgggcctggt tattgtgttg agcatctttg ggttgcagat caagaattac cgggccatct
    13981 tcatgcaagt gaaggaatcg gcttatatcg acgccgcgcg tgcctatggg gcgggcgata
    14041 tgcggatcat tttccgctat ctgatcccgc gcattctgcc tctggtcatc ccggctatcg
    14101 tgattctggt gcccagcttc gttttcctgg aagcctctct ggctgtgctg ggcctgggcg
    14161 accccttcat ccctacctgg ggcaagttga tcaacgacgc tcaaacccag ggcgcgctct
    14221 tcaacggcta ttactactgg attctgctgc cgtcggtcct gctcatgatc gccggtctgg
    14281 cgtttgccat ggtaggtttt gccctggacc gggtattcaa tccgagatta cgtgaggtat
    14341 aaggcacatg gaaaagaaaa acgaattggt tctgaaggtt cgcaaccttc gcctgtactt
    14401 caatacacgt ggcgggccgg tccaggctgt tgacggcgtc gattttgatt tgcgcaagaa
    14461 cgaagccacg gtgatcatcg gtgaatccgg ttgcggcaaa agctcgctgg ccaaggccat
    14521 cctgcgcttg ttgccgcgca acgcggggct ttatgacggc gagatatggc tccatgatgt
    14581 gaatatcatg aacatggatg ccgagaccta tcgcaaggaa attcgctgga aacggatctc
    14641 gatggttccg caggcggcta tgaacgcgct gaatccggtc atgaaaatcg gcgaccaggt
    14701 cgccgagccg gccatgatcc atatgaacat gaacaaggag gaggcgacga aatcggccgt
    14761 gcgcatgatg gagcacgtcg gtgtgcccgt tgacttcctc catcgctact cgtttgagtt
    14821 gagcggcggt atgcggcagc gctcagccct ggctatggcc ctggtgaccc ttcccgatgt
    14881 ggtcatcctg gacgagccga cgtcggcgct ggatgtgctg acccaggctt ccatcatgaa
    14941 cctgatgaag cgcatgaaaa cggagctgga gaccagcttc attctcatca cccatgacat
    15001 cgccacctcc agcgagctgg ccgacaacgt ggccgtcatg tatgccgggc aggtggtcga
    15061 ggtcagtgat gcccgtcgat tcttcactaa accgctccat ccttattccc atgcgctgat
    15121 ggccagcgtg cctcgtttgc gcgagacgat cgaaccggag ttcatcaccg gccaaccgcc
    15181 cagcctgctc aacccgccta cgggttgccg tttcaaggat cgctgcccca agcgttttga
    15241 gaagtgtgac gaagaaccgc cgaccgtcct ggttgacggc gaccggaaag tgaagtgctg
    15301 gctttatgca taagaccgtg gaaaagccaa aaccgggagg aagaccagcg aaatgacaga
    15361 cacaatgacc gagatcagcc atactgatca tgacaacaac ggccaggcat tggtgcagct
    15421 caaggatctg catgtctggt atgaattgaa aaagtttggc ttcagccatg ccggctatgt
    15481 ccgcgcggtc gataacgtca gtttcgacct taattatggc gaggccatcg cctgcgtcgg
    15541 cgagagcggc tgcggcaagt cgagtctgat gaagaccatt ctgggtctga acatccccac
    15601 caagggggac gtgatctttg acggcgcgtc gttgacacat acgaaggggg aggacttgcg
    15661 ccgctatcgc tccaagatcg gctacatcca gcaagacccg ttcggggcat tgccaccctt
    15721 catgaacgtc gagcggattc ttgaggagcc gctgatcatt aatggcgtga aggacaagga
    15781 agagcgccgc cgccgaatcg aaaagatcct cgaggaagtc aagctgacgc ctattgcgga
    15841 agtcctgccc aagttccccc acatgttgag cggtggccag cagcagcgcg tggtcatcgc
    15901 ccgggccatg atcatggaac ccaagctgct ggtagccgac gagccggtgt cgatgctcga
    15961 cgcctccgtg cgcgttgagg tgctgcgtct gttgcgcaag ctgcaggaga cccacaacct
    16021 ggccgtcctt tacatcaccc atgacctctc caccgtgcgc tacttctcgg aatacatctt
    16081 cgtcatgtat gccggagaga tcgtcgagtt cgccaggatg gacgatttgc tcgacgattc
    16141 caagcatccc tacactcatg ccttgcttga ggcgtcgtca gacccgaacg cggataatgc
    16201 acttgtccag cgcgagatac ccaagggcga gccgcccagc ctggtcaacc ctccgaccgg
    16261 ctgccgcttc cacccgcggt gcccgcaatt catgccgggc atttgcgatg tgaaggcgcc
    16321 gcccaatatc gaggtgtcac ccaagcattt ggtcgcgtgc tggctctacg acgagaaccg
    16381 aggtcatgcc ttttaatcga ttgatcgtgc ttggcttctt tatggtattg gctggggtta
    16441 tcctgccgtt cctgatcgtc atgcatgtct tgccgtctac cattctgctg aactttctgg
    16501 cttacggcgt ttctatcacc ggcgtattcc tcggagttat cggggtagcc atgtatgtgg
    16561 gcgaggagcg tcgaaagagc aaggatgatt gggatgagga tcgttagcca tcggccgaca
    16621 tcattttagt gatcaagaga cagttcgctt gccgatgggc aagcgaactg tttttgtgcg
    16681 gcatgcagtt ttctcaattg atgttgcatt tggtattatc gggttatcat tgtttatcca
    16741 ataaacgtat gcgaacggaa gcgggatatt ttgggaaata acaagaacgt tatactcgat
    16801 attaagaacc tgaaaaccca gtttttcacc gaagccgggg tggtgcgcgc tgtcgacggc
    16861 attgatttca cggtcagtcg cggcgaggtc gttggtctgg tgggcgagtc ggggtgcggc
    16921 aagagcgtga catccctctc catcatgcgc ctgattagcc agccgggcaa gatcgtcgat
    16981 ggtcaaatca tcttcgataa tcaggatctg gttgagctgc cggagtcgcg catggttgaa
    17041 atccgcggca accgcatatc gatgatcttc cagcagccgc agagcagcct gaatccggtg
    17101 ttccgcattg gcgaccaact gtcggaagtg ttgcagatcc accaggatgt aggcaaggag
    17161 ttgggcgaga aacgcgccat cgagctattg gctctggtgg gcgttcccga tcccgaatct
    17221 cgcgtgaagg ctttccccca tgagttgtcg ggcggtatgg cccagcgggt gatgatcgcc
    17281 atggcgctgg cctgcgtccc tgagttgctg atcgccgatg agccgacgac ggcgctcgac
    17341 gtgacgatcc aggctcagat cctggacctg atgcgcaatc tccgctccaa gatggacacg
    17401 gctatcatcc tgattaccca cgatctgggc gtggtggccg agatgtgcga tcgggtcaat
    17461 gtcatgtatg ccggccgcat cgtcgagcag gcgtcggtca tcgacctgtt caactctccc
    17521 atgcacccct acaccgacgc gctcattggc tcgactccgg tacttggcca tgccgataag
    17581 ccactgacta ccatccctgg ttcggttccc aacttgatca aacttccgaa aggatgcaaa
    17641 ttttcgccac gttgcgcagc acgcgtgcaa tataacctgc aaatttgcct tgaggaagaa
    17701 ccggagttga aattgctttc tcctgggcac acggtgcgct gctggctgta tgaggatttg
    17761 ccggaaatga aagacgcccc agcggcccaa tggaaggctg accgaccgga attgcataag
    17821 aggggataag cggatgcaac acgagatgag ccaacaagaa acgcgcgcga ccgctccgtc
    17881 acgaaagaag ctgatcgagg tcaataacct cgtgaaatat tttccggttc gcgccggatt
    17941 aatgcagcgc gttaaggcct gggtgaaggc tgtagatgac gtgtccttct atattaacga
    18001 aggggaaacc ctgggccttg tcggcgaatc gggttgcggc aagacgaccg tcggccgcac
    18061 gatcctgcgt ctcatcccgg ctacgtctgg agaagtcctt ctggatggta aaaacgtctt
    18121 tgatttcgac tccagcgagt tgaagaagat gcgcgccgat atgcagatca tcttccagga
    18181 cccttactcg tcccttgacc cccgtatgcc gattggcgaa tctatcgccg agggcctgcg
    18241 cgtccatacg aacatgagcg gtcaggagcg ctacgacgtc gtcacggaaa tgctgacccg
    18301 cgtcggcatg cgtgccgaac atgcccgccg ctatcctcat gagttttccg gcggtcagcg
    18361 gcagcgtatc ggcatcgccc gcgcgctggc cttgcggccg aaattcatcg tatgtgatga
    18421 gccggtctcg gcgctcgacg tgtcgattca ggcgcaggtg ctgaatatcc tgagcgaact
    18481 tcagcgcgac ttcgggctga catatctctt tatcgcccat aacctcagcg tggtcgagca
    18541 tttcagcgag cgcgtcggtg tgatgtactt gggcaagatg gtcgagatgg ccccccgcga
    18601 tttgctctac agcgacccgc tacaccccta tacacaggcg ctgatgtcgg ccatcccggt
    18661 gccggacccg acctacaagc ggcagcgtat cattttggag ggtgacgtgc ccagcccgtt
    18721 gaacccgcct tccggctgcc ggtttcatac ccgctgccca atcgcctttg atttgtgttc
    18781 acaggaagag ccaccgttca aggactatgg cggtgggcat ttcgccgcct gccactggct
    18841 cgaagttggc ggccatggcg caagcaagct gacgcccgac aatatcgctg ccaacaaggg
    18901 cagggatgtc gcccaaaaga tgattcattg aggatgaacc gccggccgca aaccggcggc
    18961 ttttattttg aacgcgattt tcgggccggc ttcgattggc ttaaggcggt ctccacatag
    19021 ccgatgatct cgcccgcgac gtcgataccg gtcgccttct caatcccctc caggccggga
    19081 gacgagttaa cctccaacaa tagcgggccg cggtgtgaga acacgaggtc aacaccggcc
    19141 accgccaggc catgaatccg ggcggcagcc acggccatcg cgactatggc cggttcgggg
    19201 acgaaggccg atgccgaacc gccgcgatgg agattcgctc gaaactctcc cgccggagcc
    19261 tgacgctgca tggccgccac gcagcggtct cctaccacga tgagtcgcat gtcgcggccg
    19321 tctgcctcgg cgatgaactc ctggacgatg gcctgccggt tcagttcggc cactttctgc
    19381 aggatggccg cgactgtggc gaactgatga gccagaaaca ccccacggcc ctgagtcccg
    19441 ctgatgagct tgacgaccgc cggcagtccc ccgatggcct cgatggccga gaagagggct
    19501 tccggtcggg cgacgacggc cgtgcgcggt atcggcagac cgacctggga catgagttgc
    19561 aggctttgca gtttgtcgcg ggaacgggcg attgcgctcg atgaggccgt cgtcagcacg
    19621 ccggccgtct cgaactgacg cactacggct aatccataga aagtcactga tgtgccgatg
    19681 cgggggatga cggcgtcgag catgggcagc tttgtcgttt cggctgtacc gggctgcgag
    19741 gcgacaagac gttggggcac cgctgccgaa ttattgttgt ggccgatgtg gaccgcgaca
    19801 gtcgtcgtat cgatgatctc cacccggtgg ccacgttcgc gcgctgtttc cgccagccgc
    19861 cgcgtgctat agagtcccgg attgcgtgag aggattccga ttcgcatggt gtcgatgatt
    19921 gtccatccga ccgcgaactt aggccaccat tcgatgccat ggcgatcaga tatagggcca
    19981 accggacgga ttaggcggat tttcgcccag ccggccgctt tgaccggcta cgaccttatc
    20041 gttacaatcc gccctgattg gagcatcttc aatgattatt gatactttcg tcattgtgcc
    20101 gacgtataac gaggccgaca atctggacga gttgctggag cagctactgg ctctgcccac
    20161 gccaattggc gcgatcgtcg tggacgataa ctcgcccgac ggcacggggg atatggccga
    20221 gcgatgggcg acggagaacc ctggtcgagt tcatgtcatt catcgcccgg ccaagctggg
    20281 ccttggcacc gcctatatcg ccggcttcaa gaaggccatc aatgatcttg ccgccgagcg
    20341 aattctgacc atggatgccg acttttccca caacccacgt tacattcccg acatgatcgc
    20401 gttgagccga accaaacatg tggttatcgg ctcgcgctat gtccccggcg gcggcgcgcg
    20461 taattgcacc cgaaagcgga tttatctaag ccgtggggcg aattttgtgg cccgaactct
    20521 cctgggcctg aaagcgcgcg atgcaacggc cgggtttcgt ctctaccggc gcgaggtgtt
    20581 gcaatccatc ccgctggatg agatattttc cagcggctat tcctttctcg tagagatgtt
    20641 gttcctttgc cagcgccggg gctggcagat tggagaagtc cccatcattt ttgaagatcg
    20701 ccgcaagggg cagaccaaga tttcgcgcca ggagatattg aaagcccagt acaccgtgct
    20761 gagacttttc ctgcgtcgtt tgcgcggcgg tgagccccgt cgcccaccca tcactgtttc
    20821 atctgctata agtaatgata aaagttaact gtaatctgtg cgggcgggac gaatatgaag
    20881 tgcgtttccc ggccacgaca gacggcaatg ccctgcgggt tgatgctttt cgttgtaccc
    20941 attccggcta tggccatcat ccgcagatcg tggaatgtcg tcattgcgga ttgatatatg
    21001 ccaacccacg ctggtcgacc gatgacgtga tgagcgccta tacggccgtc caggatgaga
    21061 cctatgtcga agagaggctt ggccgcgagc tgacctttcg ccatcatcta cgccacatgg
    21121 agcgcgtgat tggccccgca gcgggtcgcg cgttgctcga tgtgggcgcc tacatcggcg
    21181 tatttgtcga ggcggcggcc gcggttggct ggcaagccca gggcgtggag ccgtcggagt
    21241 gggcggcggc cgaggcccgg aatcgtgggc ttgatgtgcg agtcggtacg ctggataacg
    21301 ttgacctgac cgaggcgtca ttcgacgtgg tgacgatgtg ggacgttatc gagcatctgg
    21361 ctgatccggc ggccgaactg gacaaagccc gcaggttgtt gcgccccggc ggctggctgg
    21421 tcgttcacac gatggacatc gatgcccctg tctcgcgcct ggccgggagc cggtggccct
    21481 ggttgatgga tatgcatttg tattacttcg gcaagcgcac tttaaggcgc atgttaagcg
    21541 atcatgggta taacgtggtg tgggacggcg cgcaacgacg ctatctaagc ctgggctacc
    21601 tggcttcacg tgtggcgggg atgaatgacg ctctgggtcg gctgtctcat gcgctggttg
    21661 gtggccttgg cctgaatcat tcggctattc ctgtcaattt tggggacttg cgaacgtata
    21721 ttggtcaacg ggtacgttaa tcacgatatg atatgatgat tgacgagatc gtgttatact
    21781 gattgaatca cttgaacaat tccggaacgc agtacatgga tccgattaag ttactcatcg
    21841 ttgacgacca tcctctcttt cgacaggggc tcgtcgatgt attggagagc gatcccggat
    21901 tgacggtggt tgcccaggcg gccgacggcg aagttgccgt tcatcatgcg attgaatacc
    21961 agccggatat tgttctgatg gacgttaatc tccccaatgc caatgggctg cgggtgacgc
    22021 gtcgcatcct gaacgaattg cccgacacca aagtgattat cctgaccggg tatgatgacg
    22081 ccgagcaggt tttccatgcc ttgcgcattg gcgcggcggc ctactgttca aaggacatcc
    22141 cccccgaagg gctactcgcc acggtttatt ccgtccatca gggtggatac gtgattcatg
    22201 gacaggtgat gtcctatgaa gaggtgttga gctgggtcca gcaacggctg gggcgatata
    22261 actaccccat cgatgaacag tcagccgata cctatactcc tctttcgccg cgagagatgg
    22321 agatcctgga tatggtcacc cgtggatcca gcaacaaaga gatcgccttg cggctcagta
    22381 ttagccagca gacggtcaag aatcacatga cagccatctt gcgcaagctc catgtcgacg
    22441 accgcacgca ggcggccgtc tatgcgcttc gtcatggttg ggtgcgcctg gacacatcgg
    22501 cctcttcctg aacgttgagc caagaaaacc tgtgagggta tatgcggagg aaatgtaatg
    22561 atgcgcttct ttaggagcga agatggacaa ggactagtcg agtacgctct ggtgctggtc
    22621 ctggttgccg ttgtggtcat cgccatactc gccctgcttg gcccgtatat cggcgattgg
    22681 ctcaataaca tcgccgggtg gttcagtcag ctgctcggca acgggggcta aggttctccc
    22741 gatgggtatt gattgattaa tcttgtgggg ggtaactttc gtggggatcg ataacgaaaa
    22801 caaccggcga gctatagacg ccgatgattt gcgcgaaatt cgggtacgtc gtgattcgcc
    22861 ttctccgggc acccgcgacg cctttttgga tctaaaaaac tcattaagcg atcggattct
    22921 gagagagatg gatccgacta tcgatctatc ccagcgctcg caggtacggc cttacgtcca
    22981 tgatcgcctg gactctcttc tggaagaacg gggcatcgtt ctgaatcgta gtgaaaagcg
    23041 gcaactattg gaagcaatcg tagctgattt gattcattcg cgaacgtgaa caaagctgat
    23101 ctcccggccg gatcatacac cacgcgaata cgtgaggtgg cgtctttttg gctggacgtc
    23161 gtctttccgc cggtttgcgc cctctgcggc cgcgtgggtt ggctattgtg cccgcaatgt
    23221 gaggcgaacc tcgccgttgt atcgcccacg atttgctatc gttgcggccg aatgagtgaa
    23281 ggcgcgcaac ctctaccaac tggccggctt tgtcgtgaat gccgcgccaa ccccttgccg
    23341 ctcagacaga tgcgcgcccc attgcggtat caggaaccca ccagcgatct catccatcgt
    23401 ttcaagtatg aggggtattc tgccctgggc cggccgctgg cgcactttct gattgccggc
    23461 tggcccgatt gggagcaaaa tcccgatctg attgtgcctg tgccgcttca tccaaagcgc
    23521 cgtcgttggc gcggttacaa ccaatcggag ctattggccc gaccgctggg gagcgcgaag
    23581 tcgatcgctg tctgtgcctc tgccttgcgc agaacccgcg acacgattcc gcaagtggga
    23641 ttagggccgg atgagcgaca atcgaatgtt cgcggagcat ttgaggcgga cgccacggtt
    23701 gtcgatggac ggcatgtcct gcttgtcgat gacgtgttga ccactggcgc gacgatgtcg
    23761 gccgcggccg aggcgttgat ggcggccggc gcggtgagcg tatcggcgta ttgtttggcg
    23821 cgggttagct agtcatggga taataggtat cagccaaaca gatagttcct gcagcatctg
    23881 agttttcagg ccggcgggcc ttttgagtac gcacccaatc tcattgaaag gagcagggat
    23941 acccattatg gaactgcaga tcttctcaac caatatggaa gtgactccac gtttgcaaaa
    24001 ctacgtggag aagaagaccg cacgacttga ccgatacatg cctaccctga cgaccatcca
    24061 ggtcgatctg gctatggaaa atactcgcag cgccgtgcag cgccacgtag ccgagatcac
    24121 catccgcgac gaacgcggga cgattctgcg cgctgaagag cgtaacagcg acatgtttgc
    24181 cgccgtggat gcggtgatcg acaaactcta ccggcaaatc gagcgctatc gcggcaagca
    24241 gaagcgcaag taccgtggca aggcgggcga ggtcgacatg ggtgagccgt tgcccattgc
    24301 cgaggaattg gaagaagagg atcgagccat cgtgcgccag aagcggtttg cgctccatcc
    24361 aatgtcggcc gaggaagcca ttgatcagat ggaactgctg ggccacgatt tctatgtgtt
    24421 cttcaattcc gaggactctg cggtgaatgt catctatcgc cgccgtgacg ggacgtttgg
    24481 actgcttcag cccgaaatgg gttagcgagt ggctttctga tggcaaatat ccttatcatt
    24541 tgtaccgcga atatttgtcg ctcgccggtc gccgcggcgc tgctgtgcga ccggctgcgg
    24601 cgacagggtt tggcggattg gacggtgcgc tcagccggca catgggccat ggagccgcgc
    24661 ggcgcttcgc gctatagtat cgacgttgtc cgtcgcgagg ggctggacct gagccgccac
    24721 cgagcgacga tggttgacga gccgcaattg gccaacgccg atcttgtcct gaccatggaa
    24781 gtcggccacg ccgaagcgct tcgcgcggaa ttccctcagc aagcgcacaa ggtattcatg
    24841 ttgacggaga tgattggcca cacctacaac attcccgatc cttacggcgg cccatatgag
    24901 gaatatgagc gaatgtatga gggcctgacg agcgtcattg atcaagggct tgatcgcatc
    24961 atcgaattgg ccagggaaaa tacaaacggc cgtggttgat tccgtccgcg gccgttttgg
    25021 tatgatgatg aattgatgga gcaggcgaag gccgacaggt tttcgctcgc ggccaaaccg
    25081 ggcttgagac tttaccaacc ttcaataaac tgtgcccatt cctgccgttg ctccagcagc
    25141 gagccaaagc ggtaggcggc cgagggatta ggctcaaatg gcatccgatg aagggtcatg
    25201 ttcatttccg ccgccaattt gccgccttta cgccggttac agggcgcgca ggccgctacg
    25261 aggttttccc atgtgtggcc gccgccttgg cggcggggaa tgacgtggtc gagggtcagt
    25321 gtgcgcaact tgcggccgca atactggcag gtgtaatcgt cgcggcgcag gatctcgcgc
    25381 ttcgacaggg cgattcgcgg ccgtggtcgc cggatcatat ggcttagttt gatgactgac
    25441 gggatttcaa attcggatgc ggcactatga atggtcccgc gaccattgat gacaatctca
    25501 gcctttccgg aaagtaccag agccaaagcc cgcttcgtgc tgcaaacgtg tatcggttcg
    25561 aacgtcacgt ttaaaatgag cactggctct tgcaaaagcc cattactgct catactgtcg
    25621 ggagtatatc gatgagagga gcaatcgtca attgctctga aagaggcttg ttcacgaaag
    25681 agaggaaact atgcaacaag acggacgtac cgcatggcta cgagatgaga ttgagaattt
    25741 gaagggcgcc ggcctgtata acaacatccg taccatcgat tcaccgatgg acgcctgggt
    25801 gacgatcgat ggtcgccggt tgctcaattt ttgcgccaac aattatctgg gtctggccaa
    25861 ccacccacgt attcggcagg cggctcaaca aggcatcgaa gcgtatggcg tggggccggg
    25921 cgctgtccgt tcaatcgccg gaaccacaac cctgcacgtc gatctggaac aggaactggc
    25981 gacgttcaag cgcgccgagg cctgcataac cttccagagc ggctttacgg ccaacctggc
    26041 cacgattccc gctcttgtcg gcccgggtga tctggtcttc tccgacgaac tgaaccacgc
    26101 cagcatcatc gatggctgcc gcctgagccg cgccgaggtg attcgctacg ctcataacga
    26161 tgtcgatgat ctgcggacca agatcgccgg gcgcgaggat tacaagcgcc ggctgatcat
    26221 caccgatggc gtgttcagca tggatggcga tattgctccg ctggacaaga tcaccgaaat
    26281 cgccgaggag ttcggcatca tgctgatggt cgatgacgcg catggggaag gggtgctggg
    26341 tcacggcggc cgaggcatcg tcgatcattt cgggctgcat ggtcgggtgg acgtcgaggt
    26401 tggcacactg agcaaggcct tcggtgtggt tggcgggctg gtggccggca aggccatcat
    26461 cattgagtgg ctgcgccagc gcggccgtcc gttcctcttc agcagcgcca tgaccgcccc
    26521 cgacgcagcg gcgtgcatcg aagcggtgaa aatcctgagc gaatcgacgg agctaatcga
    26581 ccgtctgtgg tcgaacgcca aaatgttccg cgatgagatg caggcgatgg gcttcaacac
    26641 cggctttagc cagacgccca tcgtcccggt catgttgggc gaggccacgc tggcccaaca
    26701 gttcagccgg cgcctgttcg aggaaggtgt cttcgcgatg tcgatcgctt ttccgaccgt
    26761 cgccaagggc aaggcgcgca tccgggtgat gaattcggcc gctcataacc acagcgacat
    26821 cgagatcgcg ctggagacgt tcaaccgcgt ggggcgcgag ctgggcgtta taaagtaaag
    26881 cgctcgccca attcgggcac tgtcacttgc ggaaaaccga gctgggcgcg cagtctttca
    26941 gccaattcga aggccgcgtc cggctcgcca tgaaccagga acgtttggcg tggcggccgc
    27001 ttgaagtggc ctgcccatgc gatgagctcc gagcggtcgg cgtggccgct catgccgctc
    27061 aggctctcca cttgcgctct caagtcataa tcctcgccga agatcttgat ccgcttttgc
    27121 ttttccacga tgcgccggcc aagggtattt ggcgcctgga aaccggtgat caggatagtg
    27181 ttgcggctgt cttcgacgtt gttcttcaga tggtgcaaaa tgcggcccgc ctcagccatg
    27241 ccgctggcgc tgatgatgac cgccggcgtc ttcacgaagt ttagtgcctt cgattcctgg
    27301 gtggaacgcg tgtagtgcat caggtggaag ccgaacgggt cgtggtcgcg gtcggtcatc
    27361 atcacctcgg cgacctcttc gtcgtaggct tcgggatggg cgcggaagat gcttgtcgcg
    27421 tcgatggcca gcggactgtc gacgtagacg gccagatcgc gcggcagatc accggcttcc
    27481 accaggttgt tgagtcggta gaccagctcc tgcgttcggc cgatggcgaa ggccgggacg
    27541 attaccttgc cgccctgtct gactgtgtcg cggatgacgt cctcaagttt gtgatcggta
    27601 tcttcatcgt cagggtgttc gcgatcgccg taggtgcttt ccatgatcag gatgtcggcc
    27661 tcatcgagaa agacgggatc cttcaggatg gggcgattgg cgcggccgag atcaccgctg
    27721 aagacgaggc gttgcgtcgg gccgccgtcc ttgtcctcga tgtcgagggc gacgatggcc
    27781 gaacccagaa cgtggccggc atcataaagc gtcagggcaa tgcccggcgc gacatgatag
    27841 gggcgctcgt agccgatccc gatgaacagt ttcaggctgt ccagcgcgtc gttcatggtg
    27901 tagatgggtt caagcggcgg caagttgtcg cgctcgcgct tcttgttgag gtattcgata
    27961 tccgattcct gaattctggc gctgtcgcgc agcatgatgt tgcacaggtc tcgcgtggcg
    28021 aaggtcgaga tgatgtcgcc gttgaaaccg ctcttgacca gattgggaat
//
LOCUS       DAOMQG010000008        27557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_751_length_27557_cov_10.630172, whole genome shotgun sequence.
ACCESSION   DAOMQG010000008 DAOMQG010000000
VERSION     DAOMQG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 27557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27557
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_751_length_27557_cov_10.630172"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="PLC06_01160"
     CDS             complement(<1..1779)
                     /locus_tag="PLC06_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRO89081.1"
                     /translation="MTLKPRLFPSNQNLTNDIRNALRPDRSIPALLAGTLTGILQIAL
                     SISFAALIFRGEIAADLPRGIGMALFSATIGLGITSLLTSCSAILGGNQSAPAAIMGV
                     MAAGIAGITTGGETRLATVAVAVALTTLITGVSLYGLGAFRLAGLARFLPYPVAGGFL
                     AGTGWLLVVGGIGTMAHRTFSFTGLPALFDPNTLILWLPGLILGAAILILAARIRHYF
                     LMPAVLIAIIVGFYLIARLAGLSASELSADGWLLGPFPTGGLWQPISMAELSIVDWRA
                     IWPQLPHLISAVVITTIALLLNATGLELAINRDIDLNREMRVAGVTNMAAGVGAGLVG
                     YLQLGTSTLNERMGAASRLTGFTAAAISGVTLWMGGAVLGFVPTVVFGSLLIYLGLSL
                     LWEWLVVAWKRLPTVDYLIVLLILAVIATAGFLEGVLVGILATVILFVVSYSRTSVVK
                     HTLTGTSFKSRVTRSPEDRALLEAIGDQAYLLQLQGFIFFGTANSLLDKVRDRVCDVP
                     TRYVVIDFRQVIGLDSTALLSFNKMAQTARAGNFTLVFSGLSGKLQTRFEQGGLREEA
                     GVVRYAHDLDHAAEWCEDQLCANQRDD"
     gene            complement(1909..4122)
                     /locus_tag="PLC06_01165"
     CDS             complement(1909..4122)
                     /locus_tag="PLC06_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRO89082.1"
                     /translation="MTMKSRLSPSTMDDIRNVLRPGQLFPILLAGGLIGILQIAIAAS
                     FGALIFSGEMATYLSRGIGMALFSAMLGLAITSLLTSYPAIMGGNQDSPAAIMGVMAA
                     GIAGMVTGGEARLATVVVAVALTTLITGVFLFGLGTFRLAGLVRFLPYPVAGGFLAGT
                     GWLLAVGGIGTMARRPFAFTDLPALFAADTLIYWLPGLILGAAILILSTRIRHYLFMP
                     GMIIAIAAGFYLIAALAGMSPAELSAKGWLLGPFPQGRMWQPVGMAELSAVDWRAIWS
                     QLPNLVTAVIISSIALLLNATGLELAFNRDIDLNREMRVAGLANMASGTGAGLTGFVQ
                     FSFSVLVERLGAANRLTGLLAAAVCGITLWFGGAALGFVPTVVFGALLVYLGLSFLWE
                     WVVVARKRLPLPDYLVILLILIIIAAVGFLEGVLVGIIVTVVMFVVNYSRTSVVKHEL
                     NGTTFRSRVTRNLDDRAVLDALGDQAYILQLQGFVFFGTANGLLEQVRARTHRQPTRY
                     IVLDFHQVIGLDSTALLSFDKMRQLARGQGLILVLSGLSERLRQQFTRGGIDEEPGVI
                     HFAADIDRAAEWYEDQLCAANNRNTAERRLTAWLQDIVPGEATGRLVGYMERRDVAQG
                     DHLIRQGAESEQLFFIESGQVTAQLENDGSPPVRLETMQGGRSVGELGFYLGTTRSAA
                     VVVDRPGIIYSLSRESLNHIEQEDPEAAYAFHRIIVHLLGERVLHLVRTMDALQS"
     gene            4292..5458
                     /locus_tag="PLC06_01170"
     CDS             4292..5458
                     /locus_tag="PLC06_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRO89083.1"
                     /translation="MVEQSKPLLFFLVGLASVFIIISGIRGLAFILNPILLSLVITIT
                     VLPLPAWFAKRGMPGWLSLVMTFLVVLGMLGLVLALVFFGIAQLGDVVPTLQATTITE
                     PATIIEGTPDELIGIQVSYIQTILEEFVTSQRITSMVSWLVGALFIGLSQAFLVMLIF
                     AFMLSSALAMPSSARLGLRTDHPLLLQVANLTADVRRYITLTTALNFLTGLGNTILLL
                     FLGIGPALLWGILSGFMGYIPAVGFWVAMIPPLVLGFIQGGPTQALMVLIGYALINGT
                     VENLVKPRILGQQLKISPAVVVIALFVWGWLLGAVGAILSTPLTLLILSILEGFDSTR
                     WITILLRTAGTGESADESKERAAAVGRMRGLWDKAVTTVRNGTTSPAKRADEPE"
     gene            complement(5499..6269)
                     /locus_tag="PLC06_01175"
     CDS             complement(5499..6269)
                     /locus_tag="PLC06_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRO89084.1"
                     /translation="MPLDPLQYLLIALAAVAAGAVNALAGGGTLITFPALTSVGVPTV
                     AANITNTVALSPGYLGATLAQRVEILDQRRRLMYLLPAATLGGLVGGFLLLNTSEKLF
                     RQLVPFLILLASLLLAVQEPLRRRLVARAARSGGGARHEGWAAVPVLFAAIYGGYFGA
                     GLSVIVLAVLGLSLDDNLTRLNALKQIISFTTNMTAALLFLFSGQVVWSAAAVMAVGA
                     MIGGAMGGRLAGRVKPATLRLVVVTIGIIIAIIYFIRG"
     gene            6645..6929
                     /locus_tag="PLC06_01180"
     CDS             6645..6929
                     /locus_tag="PLC06_01180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRO89085.1"
                     /translation="MASPKRILVACGTAIATSTVVAIAIEEAMKARGIPVITRQCKAT
                     EVRGLAPGYDLIVTTTPVPKDLNVPVIQTLAFLTGVGKEAVIEQIANALK"
     gene            6999..8396
                     /locus_tag="PLC06_01185"
     CDS             6999..8396
                     /locus_tag="PLC06_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRO89086.1"
                     /translation="MDTFLNAIKSVFDNLGATVLLPIIIFFLALALGTKPGRAFRSAI
                     TIGIAFIGINLVFGLMWSTLTEVGQAMVTNTGLERNVIDVGWPAAAAIAFATQVGLWV
                     IPIAILVNVVLLLARLTKTLNIDVWNFWHFAFIGSFVAIASKNFALGLFAAAVAAAWA
                     LFFGDWVAKAIQHFYGLPGISIPHMTTGPGVPFAVATNWVIERIPGANKINWDPDSIQ
                     KRWGVFGDPIVLGLLIGLGLGLLAFYNVGSLLEMTVKVLQTAMNLAAVMLLLPRMVQI
                     LMEGLIPISEAARDFLQKRASNREIYIGLDSAILIGHPSAISATLLLVPIAILLSLIL
                     PGNQVILFADLAVIPFVVAGFAPIMRGNIFRMVIAGIVVLGLGFYIGTALAPSMTAAA
                     VEANFTMPEGAAQIISIADGFLWPPFVFHWLGRSLGWIGIGLLAVLVLVVMYFFQRNP
                     GPWERLAGAPAEGED"
     gene            8539..8844
                     /locus_tag="PLC06_01190"
     CDS             8539..8844
                     /locus_tag="PLC06_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89087.1"
                     /translation="MAKLEVSRAFPDISADRLYAGFEPAFAKAGFQPQKLRPIGWLAL
                     ATRTDVGGSIQANLSTRPGNPASATLIMIGTRQTMEELQSLAEVVWAALATYLEANV"
     gene            8837..9253
                     /locus_tag="PLC06_01195"
     CDS             8837..9253
                     /locus_tag="PLC06_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89088.1"
                     /translation="MFDIFIRSLLGPWGQNILDAYIANSLWINGLLLLYFAILVLSRW
                     NYRRTQAAIVAGIQARYAQSSGKKATRDIRARLAQDGVPWEVGLGASFWPLIAGPRHV
                     IPRLKNTPTLQKLFSPDDLIDLYVNQVALATTSENE"
     gene            9272..9751
                     /locus_tag="PLC06_01200"
     CDS             9272..9751
                     /locus_tag="PLC06_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRO89089.1"
                     /translation="MATSRILDLLVPAAVCVQLNAADSRDVIATLGQRLHAAGYVRDT
                     YVQAALDREAQMPTGLPLDGEYNVAIPHTDIEHVIKPALAMATLANPVEFRNMIDPDD
                     IVPVQLVILMAMEEAKSQVEMLQQIAGMLQDPDLIAELVAANDEEEVRRALAGAEPV"
     gene            complement(9789..>10586)
                     /locus_tag="PLC06_01205"
     CDS             complement(9789..>10586)
                     /locus_tag="PLC06_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRO89090.1"
                     /translation="TEEEQYEAYSRAARQMGDRPLIIRTLDVGGDKPLPYLDLGDEAN
                     PFLGWRAIRFCLQRPDIFMPQLRAILRAGCDGSTPLNVKIMFPMVGTLAELREAKSML
                     DEARRQLRREGTSFNEKMDVGIMIEVPSAVAIADQLAREVDFFSIGTNDLTQYVMAAD
                     RGNARVAHLASALQPAVLRSVRDAAEAAHAAEIWIGMCGELAGNPLAAPVLLGLGLDE
                     LSMSAPAIPAVKEAVRGLTMEEARRIAEEVLNMESAEAIGRFLEKPA"
     assembly_gap    10587..10686
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10687..11482)
                     /gene="dhaM"
                     /locus_tag="PLC06_01210"
     CDS             complement(<10687..11482)
                     /gene="dhaM"
                     /locus_tag="PLC06_01210"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HRO89091.1"
                     /translation="MVGLVIVSHSARLAEGVRELAQQMTQGRVPLAIAGGIDDPDNPI
                     GTDAMKVIEAIDAVYSDDGVVVLMDLGSALLSAETALDFLPDEQRAHVFLSKAPLVEG
                     AMAAAVQAMVGAPVVQVLAEAESALSVKREQLGGDPEALPATTSAVPASAPSESVTLL
                     VRNRLGLHARPAARFVMTAGHYEADIRVAKGGKSANAKSINQVAILGARQGDEIVVTA
                     TGPDAAAALAALQALAADNFGDRDNEPADQAPLRPTDHRDSRRATTD"
     gene            complement(11454..12029)
                     /locus_tag="PLC06_01215"
     CDS             complement(11454..12029)
                     /locus_tag="PLC06_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HRO89092.1"
                     /translation="MLDDSRWETMWATYDEETYTQALAWVPRGAVVLDIGAGDLRLAR
                     RIAERVRIVYAIEQRPELIAGAPLPPNGVVIYGDARALPFPAGVDTAVLLMRHCRHFP
                     LYRAKLEAIGCERLITNARWGMGVECIDLTTPAQRYEQMEMGWYACRCGATGFRPGPP
                     EQLSEYLIEHIHEVVNCPECINHGRISHRLA"
     gene            complement(12045..12287)
                     /locus_tag="PLC06_01220"
     CDS             complement(12045..12287)
                     /locus_tag="PLC06_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89093.1"
                     /translation="MAGKKTKEARIEEEVRRGQRYLRESSRQYWIRAAIVTIGLFAVV
                     LWLNYPTRGASAFLWAFGIGGLVLGYFIVSYIMFRR"
     gene            complement(12295..12690)
                     /locus_tag="PLC06_01225"
     CDS             complement(12295..12690)
                     /locus_tag="PLC06_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89094.1"
                     /translation="MNSALPHRSLEQMAARRDRSGSLWLIQAFSGLILVILLGLHMIA
                     HHYVVEGGLRDYQQVLDYVNNPVVFILQLSFLVLGVGHGLLGVRAIITDMRPSASTLR
                     VVNWVLIVIGAVAIVYGFYLALALQRMAG"
     gene            complement(12719..13123)
                     /gene="sdhC"
                     /locus_tag="PLC06_01230"
     CDS             complement(12719..13123)
                     /gene="sdhC"
                     /locus_tag="PLC06_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRO89095.1"
                     /translation="MPDIQPTTDPARPKSSLRAWFDVRGRSVSTLAFALNRLTGLGLT
                     AYLFLHLIVLSQLLRGPEGWNDFLRIAKSPLFLMLDVVLLFGVVYHMFNGIRVALVGM
                     GMANRQQKGLLYGLVGTGLVLFVVGAVLIFTK"
     gene            complement(13159..13878)
                     /locus_tag="PLC06_01235"
     CDS             complement(13159..13878)
                     /locus_tag="PLC06_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRO89096.1"
                     /translation="MAEQMIEFKVSRYKQGQPGPTYQTFKMLCDENTTVLVALQDIRR
                     DLDDSLTLRHSCHHASCGTCGMRINGKEELSCVVKVLELGTPVVTVEPMRNLPIISDL
                     VVNMEPFYDRFNSVGLPFIRGSEFLEDAEIAEGLEAYGRYENCIECNLCMSACPIMGS
                     DETYLGPATLAAAWRVVEEPRGLPVKPLLDWVDSENGCWRCHVAYECNVACPSGVYPG
                     DTIMSLRRQLTKRKFRGLFGK"
     gene            complement(13968..15128)
                     /locus_tag="PLC06_01240"
     CDS             complement(13968..15128)
                     /locus_tag="PLC06_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO89097.1"
                     /translation="MASFHAVIIGGGGTGAAVAHDLILRGYKVTLVERGEVTSGTTGR
                     HHGLLHSGARYAVKDKESAVECIEENMILRRIAPGSFELNDGLFVALTEEEAHYSHEF
                     IEACNECGIPARRLTGEEVLAREPNVNPDVIMAVQVPDATMDAMRLPLRFFATAKHNG
                     AVIKTYTEVVDFIKRGNAVSAIRVRDHTSNKVYEIGGDIFINAAGAWSGQIGDMAGVD
                     VPIRPSPGVILAVRGRLCNMVINRLHTSGDGDIIVPQRALSVIGTSSWVVEDPDNLGL
                     PQDHIDMMIKYGSELIPAVHTAPFRAAWSAARPLIGPRGADTGRELSRTFKCFDHIDE
                     GVEGFVTITGGKATTLRGMAEATANVVCAKMGVDVVCSTRETVMLPHTAYYV"
     gene            complement(15350..>16564)
                     /gene="glpK"
                     /locus_tag="PLC06_01245"
     CDS             complement(15350..>16564)
                     /gene="glpK"
                     /locus_tag="PLC06_01245"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRO89098.1"
                     /translation="NAIVWQDTRTDKLIKDFEGNVGQNRFRDKVGLPLATYFSGPKVR
                     WVLDNVPEAKAAAARGDAIFGNIDTWVIWNLTGGTNGGAHVTDVTNASRTMLMNLKTL
                     DWDKEVADIMGVPLSMMPKIMPSSAVYGHTTESGAFGGRIPVAGDLGDQQAATVGQAA
                     LSPGEAKNTYGTGCFMIMNTGTDIVPSKNGLLTTLCYKFGEKPAVYALEGSIAITGAL
                     VQWLRDNLDFFDYSPHIEDYAKSVADNGGVYFVPAFSGLFAPYWKSDARGVIVGLTRY
                     VNKGHIARAALEATAYQTREVLDAMNADSGVPLTALKVDGGMVYNDTLMQFQADVLGV
                     PVVRPKVAETTALGAAYAAGLAVGFWKSEEDIRENWAMDKTWEPDKNSKAATENYRMW
                     KKAVTRTFDWVE"
     assembly_gap    16565..16664
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16809..17444)
                     /gene="dhaL"
                     /locus_tag="PLC06_01250"
     CDS             complement(16809..17444)
                     /gene="dhaL"
                     /locus_tag="PLC06_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016871890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HRO89099.1"
                     /translation="MTNVTKEQIVRWLELTAAVLEENKDYLTQLDSAIGDADHGTNMD
                     RGFRKVAEKLPTVADKDIGNILKTVGMTLISSVGGASGPLYGTFFQRGGMAADAKEEL
                     NDEDLLTMLQGAVDGVLARGRAQPGDKTMIDALLPAMAALRAGIAAGKGNVGAMEDAV
                     AAAEQGMKDTIPLQARKGRASYLGERSIGHQDPGATSSYLMLNALLEAIRQ"
     gene            complement(17531..18604)
                     /gene="dhaK"
                     /locus_tag="PLC06_01255"
     CDS             complement(17531..18604)
                     /gene="dhaK"
                     /locus_tag="PLC06_01255"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRO89100.1"
                     /translation="MKKLINAVPDVVKEQLEGMALAHPDELKVHFEPNYVYRVDAPVA
                     GKVAIISGGGSGHEPMHGGFVGMGMLDAACPGEVFTSPTPDQMYEAARAVNSGAGVLF
                     LVKNYTGDVLNFETAAELAYGDGIPVQNILIDDDVAVKDSLYTAGRRGVGTTVLVEKI
                     VGAAAEAGYDLDQCADLARKANQYGRSIGIALTSCTVPAAGKPTFELGENEMEFGIGI
                     HGEPGQKRMPMAPADVITEMMALEIIDDPAYSRVVREWDRDAGDWVDKSLTDEPFKRG
                     DKVIAFVNSMGGTPVSELYAVYRKLDEVCKAKGIEIVRNLIGPYITSLEMAGVSITLM
                     KVDDEILKFWDAPVKTPGLRWGV"
     gene            complement(18824..19555)
                     /locus_tag="PLC06_01260"
     CDS             complement(18824..19555)
                     /locus_tag="PLC06_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRO89101.1"
                     /translation="MPSNFMGELLGTMFLIILGDGVVANVVLAKTKGNGSGWIVITTG
                     WAMAVVIGVFIAISAGAPEADINPAVTIGKWLILGVYSASEAIMTMVAQLIGAFLGAV
                     VVWLSYFNHWGATPDPGLKLAVFSTGPAISNPLWNVVTEVVGTIVLLFGVGAIFSKAV
                     APDGLAAGLGPYLVGMLVWGIGLSLGGPTGYAINPARDLGPRIAHQILPIPGKGPSGW
                     GYGWIPVVGPLVGGVIGAIMWNMFF"
     gene            complement(19986..21971)
                     /gene="dhaR"
                     /locus_tag="PLC06_01265"
     CDS             complement(19986..21971)
                     /gene="dhaR"
                     /locus_tag="PLC06_01265"
                     /inference="COORDINATES: protein motif:HMM:NF008485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HRO89102.1"
                     /translation="MLPADIGTRNEAIRESWLSFTETGRLDAIEAARMDPAIVRSWRR
                     CVNRFNPLARPRVAPINAGALSSIMRAQLDLITVATPFIEDIHQFTEGSNYAILLADG
                     AGCILLIGGDPKAMAQATALGFHQGTYWSENNAGTNALGLVLMEAMPTQVIGAEHYFD
                     FLHQTVTAAAPIHDVHGRIIGLLGMAGALESPSFHTLGLVMSAARAIGNQFQANWYLE
                     EVNQHLSQVNTILGAITEGVIVWNQEGEIHHVNEQAAHILQINPRNVLGRPLGDVLRL
                     PPELTAAIETGSELHDVELSLQADGRDVRAVITLRPIYEAGPQPVSYVALLSPIEHVR
                     QIAQQQYGAQTGLSIEDVQADSPGMRRVVRQARTAARGTAPVLIMGEGGAGKNRLARA
                     IHNDGPRASKPFIDINCRAIPHELMVREILGAEQDTGTRGQPSKFELADGGTLLLDQI
                     ENLSLEIQAAVLHVIETQHVTRLGGSMPIPVDVRIIASTMANLEDLVGQGSFMPHLFY
                     RFGVFRLDVPPLRERVEDIPLLAERFLTRQTTRDGRAVWIEDEAVRFLSRYPWPGNVR
                     ELESALERAMNQAREGIIRPSDLPEMVRNGRVLSGRHPLAQPVISVDEAERDAILRAG
                     WACNGRVTEMAKTLGIGRSTLWRKMKQLDISPKTFKK"
     gene            22397..24301
                     /gene="ftsH"
                     /locus_tag="PLC06_01270"
     CDS             22397..24301
                     /gene="ftsH"
                     /locus_tag="PLC06_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRO89103.1"
                     /translation="MDEQPNDSRPSIPTWVWIITIFAVILGLQLWLSGKFSGPEQISL
                     QKMADHIAAGDVKQLVVSGDRLQVTLKDDRTFGAIKSPSDSLIETFEYYGISAEQLNN
                     NLVIRDQSTWNTLITIALSLGPVLLLIWIFTRGFRQMQGGGGNNIFGFGRSRARNLTD
                     ANRPTVTFDDVAGVEEAKLELAEVVQFLREPEKFVQVGARIPKGVLMVGPPGTGKTLL
                     ARAVAGEAGVPFFHISGSEFVEMFVGVGASRVRDLFEKAKAAAPSIVFVDEIDAVGRQ
                     RGAGLGGGHDEREQTLNQILVEMDGFDNETNVIVIAATNRADILDPALLRPGRFDRKV
                     FVDLPDIAGREKILQVHARGKPIAGEVNFKEVARLTAGFSGADLENLINEAAIFAARR
                     SKRTIGLLEFQDAFDRVVMGPERQTRVMSEDDKMTVAYHEAGHAVVSFFLTHTDPVQK
                     ITIVPRGRAGGYVMSLPEDRALYSREFFDDQIAMALGGRASEEHFFGRVTTGASNDLQ
                     NATRLARAMVMEYGMSDRLGLPTYGGGSGSPFVGREMGFFGSGRDYSEEAAQAIDAEV
                     KRILEENYARALGVIEQKHDRMVQLATTLMDVETLDRPAFEKLMNDPISDNGRVPDEV
                     VADAQPVEAI"
     gene            24410..25198
                     /locus_tag="PLC06_01275"
     CDS             24410..25198
                     /locus_tag="PLC06_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRO89104.1"
                     /translation="MTEMTQAVVTSRDGSVLTVSLNRPEQHNAMNPAIMHELMRIFGE
                     LPARDDVRVVVLTGNGRSFCAGADLAFMRAAADYTFEQNVADGQAIFNLMMAVDSCPQ
                     PVVGRINGAAIGGGAGLVSCCDIVVASERATFAFSEARLGIAPAVISPFVLSRIGPGH
                     ARELFLTGERFDVRRAQAIGLVHHVAAESELDAVVAERVTQLLQAAPGAQAAIKELIR
                     AVAHQPKADAREYTAETIAARRASDEGREGMSAFLEKRKPRWQE"
     gene            25260..26150
                     /locus_tag="PLC06_01280"
     CDS             25260..26150
                     /locus_tag="PLC06_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRO89105.1"
                     /translation="MKLAILSDIHGNWPALEAVAADIDAWRPDRVLVNGDVVNDGPSS
                     VACWRYVARRRHDDGWQVLRGNHEEYVTEWCDPSTPRQGPAYELIRLSQWTYEQFGGD
                     VAELAELPDRWEWAAPDGTALIAMHGTLPGNRVGIYPFTPDEIIRQRIPTAAAVFVTA
                     HTHIAHIRQLDSTLVVNIGSAGIPGDGDGRAAYGRFTWEQGCGWQAEIRRVDYDRSAA
                     ERDYFTSGFMTGVGPEAELSLVQFRMARDVRTRWSANYRERILNGEMSVMESVDQWLR
                     RDEFRGYRLADAPAPRPAVC"
     gene            26298..27122
                     /locus_tag="PLC06_01285"
     CDS             26298..27122
                     /locus_tag="PLC06_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89106.1"
                     /translation="MNYIDLLLSGTILPWLLGLLLALGLVALLMSMKLWRDTKRSPYF
                     FMRQQAARRMQSYLSAFVALMLLAVGIGVYGWRTPEDATVRMALITRAKPATAEVQEL
                     MAQPSVAERATAAAVATTTRSGGVANVLLSADEATSLVPELPAAYDQVKSLVDLKSST
                     ELGTVVLSSTVTDEYKAINPASVFGEGFYTLYATFAYKDMADGMAWSWVWRHNGQVID
                     GGNELWAYGDEGPGYIYLNPEEGFAEGRYSLEVWVNGELLGQASATMNDAAVAAGN"
     gene            complement(27210..>27557)
                     /locus_tag="PLC06_01290"
     CDS             complement(27210..>27557)
                     /locus_tag="PLC06_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89107.1"
                     /translation="FPTLGWLPLGGLALGFSLSNLLEVGLLLWLIRGKLNGLNGRSLL
                     SGLARMGVASLAMAAAVAGVLLWLPASAVWSRAIIGTAAGGVAYLLISQLLRVEEWQR
                     FVGILARRLPGRS"
BASE COUNT         5411 a         8479 c         8195 g         5272 t
ORIGIN      
        1 atcatctcgc tgattggcgc atagctgatc ctcgcaccac tcggcggcgt gatcgaggtc
       61 atgggcatag cgcactactc ccgcttcctc cctcaatccg ccctgctcaa accgggtttg
      121 cagcttcccg gacaaaccgg aaaacaccaa cgtgaagttg cccgcccgcg cggtctgggc
      181 cattttgttg aagctcaata aagccgtcga atcgaggccg atgacctggc gaaaatcaat
      241 gacaacataa cgggtaggca catcgcacac ccgatcccgc accttatcaa gcaagctgtt
      301 ggcagttccg aaaaagatga acccctgcaa ctgtaacagg taagcctggt cgccgatagc
      361 ctccagtagg gcgcgatctt ccgggctgcg ggtgacgcgg ctcttgaagc tggtcccggt
      421 cagggtatgc ttaacgacgc tggtgcggct gtaactgacg acaaacagga tgacggtcgc
      481 gagaataccg acgagcaccc cctccagaaa gccggcagtg gcgatgacgg ccaggatcag
      541 gaggacgatg aggtaatcga cagtgggcaa gcgcttccag gccacaacga gccactccca
      601 cagcagcgac aaccccagat agatcagcag cgacccaaaa acgacggtcg gcacaaaacc
      661 caggacggct ccacccatcc acaaggtcac gccgctgata gcggcggcgg tgaacccggt
      721 caggcggctg gccgcgccca ttcgctcatt cagcgtcgat gtgccgagtt gtaaatagcc
      781 cacaagtccc gctcccacgc cggccgccat attcgtcaca ccggccacgc gcatctcgcg
      841 attgagatcg atgtcgcggt tgatggccag ttccagcccc gtggcgttca gcagcaaggc
      901 gatggtggtg atgacgacgg cgctgatcag atgcggcaac tgtggccaga tcgctcgcca
      961 atccacgatc gacagctcag ccatgctgat tggctgccat aatccccccg tggggaatgg
     1021 ccccagcagc cagccgtcgg cgctcagctc cgaggccgac aaacccgcca gcctggcgat
     1081 taggtagaac ccgacgatga tggcgatgag caccgccggc ataaggaagt agtggcgaat
     1141 gcgcgcagca aggatcagga tcgctgcccc caatatcagg ccgggcagcc acaagatgag
     1201 cgtattggga tcgaacaagg cgggcaggcc ggtgaaggaa aaggttcggt gggccatcgt
     1261 gccgatgccg ccgaccacca gcagccagcc cgtgcccgcc aaaaatccac cggccaccgg
     1321 atagggcaaa aaccgcgcca gccccgccag ccggaaagca cccaaaccgt acagggagac
     1381 gccggtgatc aaggtggtca gggcgacggc aacagcgaca gtggctaatc gggtttcgcc
     1441 cccggtggtg atgccggcga tccccgcagc catgacaccc atgatcgccg ccggagcgct
     1501 ctggttgccg cccaggatgg ccgagcagga cgtcagcaag ctggtgatgc ccaggccgat
     1561 tgtggccgag aacagcgcca tgccgatgcc gcgggggaga tcggcggcta tttctcctct
     1621 gaagatgagg gccgcaaatg agatggacag ggcaatttgg aggatgccgg tcagtgtgcc
     1681 ggccagcagc gcgggaatcg atcggtctgg ccgcaatgca tttcgaatgt cattggtcag
     1741 gttttgattg gatggaaaca gccgtggctt caatgtcatg aaactcccca ccggcaacgg
     1801 cgcaggcgtt gggtttgggt aatcgaccag ggtgatatta tgccgtgtgc acctgatcgc
     1861 gcaatcctcc gtccgtcggc gccggcatct tgccgcaagt gaatgacgtc agctttgcaa
     1921 ggcatccatc gtccgcacca ggtgcagcac gcgctctccc aacagatgaa cgataatgcg
     1981 atggaacgca taggctgctt cgggatcctc ttgctcgatg tggttcaagg attcccgcga
     2041 gaggctatag atgatacccg gtcgatcgac aacaacggcc gcgctgcgcg tcgtgcccag
     2101 atagaacccc agctcaccca cgcttcggcc gccctgcatc gtttccagac gcacgggtgg
     2161 gctgccgtca ttctccaatt gcgccgtcac ctgacccgac tcaatgaaaa atagctgctc
     2221 cgattcggct ccctgccgga tcaggtggtc gccctgagcg acatcccggc gttccatata
     2281 gccgaccaga cgaccggtcg cttcgcccgg gacgatatcc tggagccagg cggtgagacg
     2341 tcgttcggcg gtgttcctgt tattggcggc acataactgg tcctcatacc attcggccgc
     2401 gcgatcgata tcggcggcaa aatgaattac gcccggttct tcatcgattc cgccgcgcgt
     2461 gaactgctgc cgcaagcgct ccgacaagcc cgacaacacc aggatcaggc cctgaccgcg
     2521 agccaactgg cgcattttgt caaagctgag aagagccgtt gaatccaggc cgatgacctg
     2581 gtgaaagtca aggacgatgt agcgcgtcgg ctgccggtga gttcgggcgc gcacctgttc
     2641 cagcaggccg ttggctgttc caaaaaagac gaagccctgc aactgaagga tgtaggcctg
     2701 atcaccgagc gcgtccagca cggcgcggtc atcgaggttg cgcgtgacgc gactcctgaa
     2761 cgtggtgcca ttcagctcat gcttgacgac gctggtgcgg ctatagttga cgacgaacat
     2821 gaccacggtg acgatgatgc cgaccagtac cccttccaga aaaccaacgg cggcgatgat
     2881 gatcaggatc aggaggatga ccagatagtc gggaagaggc agacgcttgc gggccacgac
     2941 gacccattcc cacagaaaag acaggcccag atagaccagc aaggccccaa agacgaccgt
     3001 gggcacgaac cccagcgccg ctccgccaaa ccacaaagtg ataccacaaa cggccgccgc
     3061 caatagcccg gtcaggcgat tggccgcgcc cagccgctcg accagtaccg aaaaactaaa
     3121 ctgcacgaaa ccggtcaatc ccgcccccgt gcccgatgcc atattggcca gaccggccac
     3181 gcgcatctca cgattgagat cgatgtcgcg attgaaggcc agttcaagcc cggtcgcgtt
     3241 cagcagcaag gcgatggaac tgatgatgac ggcagtgacc agattgggca actgcgacca
     3301 aatcgcccgc cagtccacgg ccgacaactc agccatgccg acaggttgcc acatccgccc
     3361 ctgggggaat ggccccagca gccatccttt ggcgctcagt tccgccggcg acatgcccgc
     3421 cagcgcggcg attagataga acccggcggc gatagcgatg atcatcccag gcatgaacag
     3481 gtaatgacga atacgagtag agaggatcag gatcgctgct cccagtatca acccgggcaa
     3541 ccaatagatg agcgtatcgg ccgcgaacaa ggcgggcaga tcggtgaagg cgaacggccg
     3601 gcgagccatc gtgccgatgc cgccgacggc cagcagccac cccgtccccg ccagaaatcc
     3661 acccgccacc ggatagggca aaaaccgcac cagacccgcc agacgaaatg ttcccagccc
     3721 aaacaggaag accccggtga tcaaggttgt cagagcgacg gcgacgacga ccgtcgccag
     3781 ccgtgcctcg ccccccgtga ccatgccggc gatccccgcg gccataacgc ccatgatcgc
     3841 tgccggggag tcctgatttc cgcccatgat ggccggatag gatgtcagca ggctggtgat
     3901 ggccaggcca agcatggccg agaacagagc catgccgatg ccgcgcgaga gataggtcgc
     3961 catttcgcca ctgaagatga gcgcgccaaa cgaggcagct atagcgatct gaagaatgcc
     4021 gatcagcccg ccggccaaca ggatggggaa caactgccca ggacgaagca cgttgcgaat
     4081 gtcgtccatg gtagatgggg atagccgtga cttcattgtc atgataattc tctggcaaat
     4141 agcacaggcc cttgatgata aaggaaaccg gtaggcagat attatgtcat gtactcccgg
     4201 ttacgcaaaa actttcaatt ccgcatcact gggtgtatga tcaccgacca agtttgaagc
     4261 atatcattca tgatggacgg acagtgatgt tatggtagaa cagtcgaaac cgttgctgtt
     4321 ttttctcgtt gggttggcca gcgtcttcat aatcatctcc ggcattcgcg gtctggcgtt
     4381 catcctgaat cctatcctgc tgtcgctggt catcaccatc actgtgcttc ctctgccggc
     4441 ctggttcgcc aaacggggaa tgcccggctg gttgtcgctg gtgatgacct tccttgtagt
     4501 cctgggaatg cttggcctgg tattggcgct tgtattcttc ggcatagccc agctgggcga
     4561 tgttgtcccg actttgcagg cgacgaccat aaccgaacct gccacgatca tcgaggggac
     4621 gccggacgag cttatcggca tccaggtcag ctacatccag actattcttg aggagttcgt
     4681 caccagccaa aggattacca gcatggtttc gtggctggtg ggcgcgctgt tcattgggct
     4741 gtcacaagcc ttcctggtga tgctcatctt cgccttcatg ctcagttcgg cgctggccat
     4801 gcccagcagc gcgcggctgg gtctgcgcac cgaccacccc ctgctcctgc aagtggcgaa
     4861 tctgacggcg gatgtgcgtc gctacatcac cctgacgacg gcgctgaact tcctcaccgg
     4921 tctgggcaac accatcctgc tcctgtttct gggcatcggc ccggcgctct tgtggggtat
     4981 tttgtccggc ttcatgggct atattccggc tgtcggcttc tgggtcgcca tgatcccgcc
     5041 gctggtgctg ggctttattc agggcgggcc aacccaggcg cttatggtgc tgatcggcta
     5101 cgcgctcatc aacggcacgg tcgagaatct ggtgaagccg cgcatcctgg ggcagcagtt
     5161 gaaaatctcc ccggcggtcg tggtgatcgc cctgttcgtg tggggctggt tgctgggcgc
     5221 ggtgggggcc attctctcca cgccgttgac cttgctcatc ctgagcatcc tggaaggatt
     5281 cgacagcaca cgctggatca cgatcttgct gcggacggcg gggacaggtg aatcggccga
     5341 cgaaagcaag gagcgcgcgg ccgcagtcgg acgcatgcgt ggcctgtggg acaaggcggt
     5401 gacgactgtc cgcaatggga caacctcgcc cgcgaagagg gccgacgagc cggaataatc
     5461 gcgctgcctg cgggggagta tctcggccgc cgcaaaagtt acccgcgtat gaaatagatg
     5521 atggcgatga tgatgccgat ggtgacgacg accaggcgca acgtcgccgg cttgacgcgc
     5581 ccggccagcc gcccgcccat cgccccaccg atcatcgcgc cgaccgccat gaccgccgcc
     5641 gccgaccaca cgacctgccc cgaaaaaaga aacagcagcg ccgcggtcat gttggtggtg
     5701 aaggatatga tctgtttcag cgcgttcagc cgggtcagat tgtcgtcgag gctcagcccc
     5761 agcacggcca ggacgataac gctcagcccc gccccaaaat agccgccgta gatggcggcg
     5821 aacaggacgg gcacggccgc ccagccctcg tgtcgcgccc cgccgcccga acgcgctgcc
     5881 ctggcgacca gtcggcgccg cagcggctcc tgcacggcca gcaacagcga agccagcagg
     5941 atgagaaagg gcaccagttg gcgaaacagc ttctcgctcg tattgagcag caagaaaccc
     6001 ccaaccagtc cacccaatgt agccgcgggt aacaaataca tcagccgccg gcgctgatcg
     6061 aggatttcca cccgctgagc cagcgtggcc cccagatagc cggggctgag ggccaccgtg
     6121 ttggtgatat tggcggccac ggtggggacg ccgaccgagg tgagcgccgg aaatgtgatc
     6181 agcgtgccgc cgccggccaa cgcgttgact gcccccgcgg cgacggcggc gagagcgatg
     6241 agaagatact gtaacggatc aagaggcata ggtgactccg gttccaattc tgcctgccgg
     6301 atttcaagca gtctgtccag catccctcac attgtagctc tctcgtgatg ccgcgtcagc
     6361 caaaaacacc tcacccaccg ggtcgatggt tcgtaaccaa tgacagccgc atggtgttgt
     6421 attgattata tgggttatct ttgggccaat ggagtcgccc attgattctg tataggttga
     6481 ttagaccggc taatcacgct tgcagggaag ttcgccaatc cattaccccc gatggatcaa
     6541 cggcgcaagc tgcagaaaat cacatatttg acgatctctc cccgcgggct tgacaagctt
     6601 ggttacccta tataattaga gaatcattaa tttaaggaga tgatatggca tcccctaaac
     6661 gaattctggt cgcttgcggg acagcgatcg caacctcaac cgtcgtcgct atcgcgatcg
     6721 aagaggcgat gaaagcgcga ggcatccccg tgatcacgcg ccaatgcaag gcgaccgaag
     6781 tcaggggcct ggctcccggc tatgacttga tagtgacgac taccccagtg ccgaaagacc
     6841 tcaatgtccc tgttattcaa acattggcgt ttctgaccgg cgtcgggaag gaggcggtta
     6901 tcgagcagat tgccaacgca ttgaagtaaa caaataccgc ttcgcggatc cttgcatcgt
     6961 tcattgtctg ttctttaagc cttgggagac ttcatgttat ggatactttt ctaaacgcca
     7021 ttaagtctgt tttcgataac ctgggcgcca ccgttctgct ccccattatc attttcttcc
     7081 tcgctctcgc attagggaca aaacccggcc gggcttttcg ttcggcgatc accatcggca
     7141 tcgctttcat tggtatcaac ctggtattcg ggttgatgtg gagcacacta accgaagtgg
     7201 gccaggctat ggtcacgaat accggcctgg aacgtaacgt tattgacgtc ggctggccgg
     7261 ccgcggccgc catcgccttc gccacccagg tcggcctgtg ggtcatcccc atcgccattc
     7321 tggtcaacgt ggtcttgttg ttggcgcgcc tcaccaagac gctcaatatc gacgtgtgga
     7381 acttctggca ctttgccttt atcggttcgt tcgtcgccat tgccagcaaa aacttcgcct
     7441 tggggctgtt cgccgccgcc gtggccgccg cttgggcgct tttctttggt gactgggtcg
     7501 cgaaagccat ccagcatttt tatggcttgc ccggcatctc catcccccac atgacaaccg
     7561 gccccggagt ccctttcgcc gtcgccacaa actgggtgat tgagcgaatc cccggcgcca
     7621 ataaaatcaa ctgggacccg gacagcattc agaagcgctg gggcgtcttc ggcgacccca
     7681 tcgttcttgg cctgctgatc ggtttgggtt tgggtctgct ggctttctat aatgtgggat
     7741 cgcttttgga aatgacggtc aaggtgctgc aaacggccat gaatttggcg gcggtgatgt
     7801 tgctgttgcc gcgcatggtt cagattctga tggaaggcct gattcccatc tccgaggcag
     7861 cccgcgattt cctgcagaag cgcgcgtcca atcgcgagat ttacatcggc ctcgactcgg
     7921 ccatcctgat tggccatccg tcggccattt cggcgaccct gctgctggtc ccaatcgcca
     7981 ttctgctcag cctcattctg cccggcaatc aggtcatcct gtttgccgac ctggcggtca
     8041 tcccgtttgt ggtggccgga ttcgcgccaa tcatgcgcgg caatatcttc cgcatggtca
     8101 tcgccggcat cgtcgtgttg ggcctgggct tctacatcgg cacagcgctg gccccgtcca
     8161 tgaccgctgc cgcggtagag gccaacttca ccatgcccga aggcgcggcg cagatcatca
     8221 gcatcgccga cggctttttg tggccgcctt ttgtgttcca ctggttaggg cgctcgctgg
     8281 gctggatcgg catcggcttg ttggccgtgc ttgtgctggt cgtcatgtat ttcttccagc
     8341 ggaacccggg gccgtgggag cggctggctg gtgcgcctgc tgagggggaa gactgatttc
     8401 agcacgatcg ttgtaatcgg tgtaaggatc gcaaccggta attaaccgga acgatcgatg
     8461 atgaaggatc attaagaggc cggtcggggc gagaagcgta tcgccccaac cggccgatcg
     8521 attggagaag caacatccat ggcgaaattg gaagttagtc gcgcttttcc cgatatatcg
     8581 gccgatcggc tttatgccgg gttcgagccg gccttcgcca aagccggttt ccagccccag
     8641 aaattacgcc ccatcggctg gttggccttg gcaacgcgga cggatgtcgg cggcagcatc
     8701 caggccaatc tgtcgacccg acccggcaac ccggcctcgg cgacattgat catgatcggc
     8761 acccgccaga cgatggaaga gctgcaatcc ctggccgagg ttgtctgggc agcgctggca
     8821 acgtacctcg aagccaatgt ttgacatctt catccgttca cttttagggc cgtggggcca
     8881 gaatatcctc gacgcctaca tcgccaatag cctgtggatc aatgggctgc tcctgctcta
     8941 cttcgcgata ttggtgctct cgcgctggaa ttaccgccga acgcaggcgg caatagtggc
     9001 cgggatccag gcgcgctacg cccaatcaag cggcaagaag gccactcgcg acatccgtgc
     9061 ccgcctggca caggatggcg tgccgtggga agtcggcctg ggcgcttcct tctggccgtt
     9121 aatcgccggg ccacgccatg tgattccgcg acttaagaat acgcccacgc tacaaaaact
     9181 cttctcgccg gacgacctaa tcgatcttta tgtaaatcaa gtggctcttg ccacaacgtc
     9241 cgaaaatgaa taaggctgat catcatcgct tatggctact tcaagaatcc tcgacctgct
     9301 cgtccctgcg gcggtttgtg tccaactgaa cgctgccgat tcccgtgacg ttatcgccac
     9361 cctcggccaa cgtctccatg cggccggcta cgtgcgtgat acctatgtgc aggcagcact
     9421 cgaccgcgaa gcgcaaatgc ccaccggatt gccgctcgac ggcgaataca acgtcgccat
     9481 cccccatacc gatattgaac atgttatcaa gcctgcgctg gccatggcaa cgctggccaa
     9541 cccggtcgaa tttcgcaata tgatcgaccc cgatgatatt gtgcccgtgc aattggtgat
     9601 attgatggcc atggaagagg ccaaatcaca ggtggagatg cttcagcaaa tcgccggtat
     9661 gttgcaggac cccgatctca tcgcggagtt ggtggcggcc aatgacgagg aagaggttcg
     9721 gcgggccttg gcgggcgctg agccggtgtg agcgtttgaa gcggttgctc agattcgccc
     9781 ggtcaacttc aggctggttt ttccaggaaa cgaccgattg cctcggccga ttccatgttt
     9841 aatacttcct cggcaatccg ccgcgcttct tccatcgtca ggccgcgcac ggcctctttg
     9901 accgccggga tagcgggcgc gctcatgctc agctcgtcga gacccaggcc gagcaatacc
     9961 ggcgcggcca gcggattgcc cgccaattcg ccgcacatgc cgatccagat ctccgccgca
    10021 tgagcggcct cggccgcgtc gcgtacgctg cgcagcaccg ccggctggag cgcactggcc
    10081 agatgggcta cgcgggcatt accgcgatcg gcggccatca cgtattgggt caggtcgttg
    10141 gtgccgatgc tgaagaaatc gacctcgcgg gccagttggt cggcgatggc cacggccgac
    10201 ggcacttcaa tcatgatgcc aacatccatc ttttcgttaa aggaagtccc ttcccggcgc
    10261 aattgccgtc ttgcctcgtc gagcatgctt ttggcctcgc gcagttcggc gagtgttccg
    10321 accatgggga acatgatctt cacgttcaat ggggtgctgc cgtcgcagcc ggcgcgcagg
    10381 atggcgcgca gctgcggcat gaagatatcg ggtcgttgca ggcaaaagcg aatggcccgc
    10441 caaccgagga acgggttggc ttcatcgccc aggtcgagat aaggcagggg cttgtcgccg
    10501 cccacgtcga gcgtgcggat gatgagcgga cggtcgccca tttgccgggc cgcccgtgaa
    10561 taggcttcgt actgttcttc ttcggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnggtc agtggtcgcg cgacgggagt cgcggtggtc agttgggcgg agaggggcct
    10741 ggtctgccgg ttcgttgtcc cggtcgccga agttgtcggc ggccagagct tgcagcgcgg
    10801 ccagagccgc ggcggcgtcg gggccggtgg cggtcacgac gatctcgtcc ccttgccgcg
    10861 cacccaggat cgccacctga ttgatgctct tggcgttggc cgatttgccg cccttcgcta
    10921 cgcggatgtc ggcttcataa tgtccggcgg tcatcacgaa acgggcggcc ggtcgggcgt
    10981 gtaaccccag gcgattgcgg acgagcaagg tcacactttc gcttggggcg ctggccggaa
    11041 ctgcgcttgt tgtggccggc aatgcttccg gatcgccgcc caactgctct cgcttcacgc
    11101 tcagcgcgct ctcggcctcg gccaacacct ggacgaccgg cgcgccaacc atcgcctgga
    11161 cggcggcggc catcgccccc tcaaccagcg gcgctttgct caggaatacg tgcgcgcgct
    11221 gctcgtcggg cagaaaatcg agcgccgtct cggcgctcaa cagggcgctg cccagatcca
    11281 tgagaactac aacgccgtca tcgctgtaga cggcgtcgat ggcctcgatg accttcatcg
    11341 catccgtgcc gatagggtta tcgggatcgt caattccccc ggcaatggcc aatggcacgc
    11401 gcccctgggt catttgctgg gccaattccc gcaccccctc ggccaggcgc gcgctatgcg
    11461 agacgatgac taatccgacc atgatttata cactcagggc aattgactac ttcgtggatg
    11521 tgttcgatca aatattcgga aagctgctcc ggtgggccgg gccgaaagcc ggtcgcacca
    11581 caacggcagg cataccagcc catttccatc tgctcgtacc gttgggccgg ggtcgtcaga
    11641 tcgatgcact cgacccccat cccccagcgc gcgttcgtga tcagccgctc acagccaatg
    11701 gcctccagct tcgcgcgata gagggggaaa tggcgacaat ggcgcatgag cagtacggcc
    11761 gtgtcgactc ccgccggaaa gggcagggcg cgcgcgtcgc cgtaaatgac gacaccattg
    11821 ggcgggagtg gcgctccggc aatcagttcg gggcgttgct cgatagcgta tacgatgcgc
    11881 actcgctccg ctatgcgccg cgccagccgc aaatcgcccg cgccgatgtc gagaacgacc
    11941 gcgccgcgcg ggacccaggc cagcgcctgc gtgtatgtct cctcgtcata ggtcgcccac
    12001 atcgtctccc agcgtgaatc gtccagcacc gtcattattg tggactaccg cctgaacatg
    12061 atgtagctga caataaaata acccagaaca agcccgccga tgccgaaggc ccacaggaag
    12121 gccgatgcgc cgcgggtggg atagttcaac cacaacacga cggcgaacag gccgatggtg
    12181 acgatcgctg cgcggatcca gtattggcgc gaggattccc gaaggtagcg ttgacccctg
    12241 cggacctctt cctcgatgcg ggcttccttt gtcttttttc cggccatatt actcctaacc
    12301 cgccatacgc tgcagggcca gcgccagata aaagccatag acaatggcga ctgcgccaat
    12361 gacgatcagt acccaattga cgacgcgcaa tgtactcgcg ctcggccgca tgtcggtgat
    12421 gatcgcgcgc acgcccagca ggccatggcc cacgccaagg accagaaatg ataactggag
    12481 gataaacacg acaggattat tgacataatc cagtacctgc tgatagtcgc gcagtccgcc
    12541 ttccacaacg tagtgatggg cgatcatatg caggcccaga aggatcacca aaatcaggcc
    12601 ggaaaaggcc tggataagcc agagcgagcc ggagcggtca cgcctggcgg ccatttgttc
    12661 cagcgagcga tgaggtaatg cgctattcat atgaatgact ccttgatcgc gatcgatgtc
    12721 acttggtgaa gatgagaacc gcgccgacga cgaacagaac cagaccggtg ccgaccagcc
    12781 catagagcaa tcccttctgc tgccggttgg ccatgcccat gccgaccagc gccacgcgaa
    12841 tgccgttgaa catgtggtag accacaccaa acagcaggac gacgtccagc atcagaaaca
    12901 gcggcgactt ggcgatgcgc aggaaatcgt tccagccttc cggcccgcgc agcagttggc
    12961 tgagcacgat cagatgcagg aacaaataag ccgtgaggcc aagccccgtc aggcgattga
    13021 gggcgaaagc cagcgtgctg acgctgcggc cgcgaacgtc gaaccacgcc ctgagggatg
    13081 atttaggccg cgccggatcg gtggtcggtt ggatatcggg cataattgct tctcccagaa
    13141 taatgacgag tgacgaattc actttccaaa gaggccgcgg aacttgcgct tggtcaattg
    13201 gcggcgcagg gacatgatcg tgtcacccgg atagactccc gacgggcagg cgacgttgca
    13261 ttcataggcc acgtgacagc gccagcagcc gttttcgctg tctacccaat cgaggagcgg
    13321 cttcaccggc agcccgcgcg gttcctcgac cacgcgccag gccgcggcca gcgtcgccgg
    13381 ccccaggtac gtttcgtcgc tgcccatgat ggggcaggcc gacatgcaca agttgcactc
    13441 gatgcaattt tcgtagcgac cgtaggcctc cagcccctca gctatctcgg catcttccag
    13501 aaattcgctt ccgcgaatga acggcaggcc gaccgaattg aaccggtcat agaacggctc
    13561 catgttgacc accagatcgc tgatgatcgg gaggttgcgc atcggctcaa cggtgacgac
    13621 cggcgtgccc agttcgagca ccttgacgac gcaactcaac tcctccttgc cgttgatgcg
    13681 catgccgcac gtgccgcaac tggcatggtg gcaggagtga cgcagagtga gcgagtcatc
    13741 cagatcgcgc cgaatatcct gcagagccac caggacggtg gtgttctcgt cgcagagcat
    13801 tttgaaggtc tggtaagtcg gtccaggctg gccttgcttg tagcgcgaga ccttgaattc
    13861 aatcatttgt tcggccatga atggaacctc tctcaatacg accgcagatg accgaccacg
    13921 gaccgcagca agacaggtgc actgcggtcg gccgttggtg atcatcttta tacatagtag
    13981 gccgtgtgcg gcaacatgac ggtctcgcga gtggagcaaa ccacgtcgac gcccatcttc
    14041 gcgcacacca cattggccgt cgcttcggcc atgccgcgca aggttgtcgc cttgccgccg
    14101 gtgatcgtga cgaacccttc gacgccctcg tcaatatggt cgaagcattt gaaggtacgg
    14161 ctcaactcgc ggcctgtatc ggccccgcgc gggccgatga gcggccgcgc cgccgaccag
    14221 gctgcccgga agggcgcggt gtgcacggcg ggtatgagtt ccgagccgta cttgatcatc
    14281 atgtcgatgt ggtcttgcgg cagccccagg ttatcgggat cttccaccac ccacgagctg
    14341 gtgccgataa ccgacagcgc ccgctgcggg acgatgatgt cgccgtcgcc ggacgtatgg
    14401 agccgattga tgaccatgtt gcacaaccgc ccgcgcacgg ccagaatgac gcctggcgag
    14461 ggccgaatcg ggacatcgac acctgccatg tcgccgatct gaccgctcca tgcgccggcg
    14521 gcattgatga aaatatcgcc gccgatctcg tataccttgt tggacgtgtg gtcgcgcaca
    14581 cgaatggcgc tgacggcatt gccgcgcttg atgaagtcga ctacttccgt atatgttttg
    14641 atgactgcgc cgttgtgctt ggccgtggcg aagaagcgca gcggcaggcg catggcgtcc
    14701 atcgtcgcat cggggacctg aacggccatg atgacatcgg gattgacgtt gggttcgcgg
    14761 gccagcacct cttctccggt caggcgacga gcgggaatgc cgcactcgtt acaggcttcg
    14821 atgaattcgt ggctgtaatg ggcctcttcc tcggtcaggg caacgaataa tccgtcattg
    14881 agttcgaatg agccgggggc gatccgtcgc aggatcatgt tttcctcgat gcactcgacg
    14941 gccgactcct tgtcctttac ggcgtagcgc gctccgctat ggagcaggcc atggtggcgg
    15001 ccggtcgtgc ccgacgtcac ttcaccgcgc tcgaccagcg taaccttgta gccgcgcaat
    15061 atcagatcgt gggcgacggc tgcccccgtt cccccgccgc cgatgatgac tgcatgaaat
    15121 gacgccatcg tatcctcgct tgtcgcatcg ttttccgggc gacgtgtcct tcgttgcccg
    15181 tgcccccgat gctacaatgg tctccctgaa ataacaatca gagatcggac tgtggccgcc
    15241 cactgcccga tctctgttgc gctaaaccgg caggtaagca gcgcctcgct gccgatgagc
    15301 tggacgatcg ccaacggccg gatggtcgcc ggcaatcgtc catgctatct tattctaccc
    15361 aatcgaaggt gcgcgtgacg gccttcttcc acatccgata gttctcggtc gccgctttgc
    15421 tgttcttgtc cggttcccag gtcttgtcca tggcccagtt ctcgcggatg tcttcctcgc
    15481 tcttccagaa gccgactgcc agaccggcgg cataggccgc gccgagggct gtcgtctccg
    15541 ccaccttcgg ccgaacgacc ggcacgccga ggacgtccgc ctggaactgc atgagggtgt
    15601 cgttatagac catgccgcca tcgaccttca gcgccgtcaa cggcacgccg gagtcggcgt
    15661 tcatggcatc gagcacctct cgggtctgat acgcggtcgc ctcgagagcg gcgcgggcga
    15721 tgtggccctt gttgacgtag cgcgtcaggc cgacgatgac gccgcgggca tcggacttcc
    15781 agtagggcgc gaacaggccg gaaaaagcgg gcacaaagta gacgccgcca ttgtccgcaa
    15841 cggacttggc gtaatcctca atgtgggggc tgtaatcgaa gaagtcgagg ttgtcgcgca
    15901 accattggac cagagcgccg gtgatggcga tcgagccttc gagcgcgtag acggccggct
    15961 tctcgccgaa tttgtagcac agggtcgtca gcaagccgtt cttggacggg acgatgtcgg
    16021 tgccggtgtt catgatcatg aaacagccgg tgccataggt gttcttggct tcgccggggc
    16081 tgagggctgc ctggcccacg gtggccgcct gctggtcgcc caggtcgccc gccacgggga
    16141 tacgaccgcc aaacgcgccg ctctcggtcg tgtggccata gacggccgac gacggcatga
    16201 tcttgggcat catgctcaac ggcacgccca tgatgtcggc gacttccttg tcccagtcga
    16261 gggtcttgag gttcatcagc atcgtgcggc tggcgttggt gacgtcggtg acatgggcgc
    16321 cgccgttggt gccgccggtc aggttccaga tcacccaggt gtcgatgttg ccgaagatgg
    16381 cgtcgccgcg cgcggccgcg gccttggctt cgggcacatt atcgagaacc cagcgcacct
    16441 tcgggccgga gaaataggtg gccagcggca ggccgacctt gtcgcggaac cggttctggc
    16501 ccacgttgcc ctcgaaatcc ttgatgagct tgtcggtgcg ggtgtcctgc cagacgatgg
    16561 cattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttaaaa atcatgcaac
    16681 gggtactggt ggtaccctgg tcgattgctg ctacataatc cgccatttta aactctcctt
    16741 tgctatcgtt ggttcgtgaa tcagtgggtg tcaacgaccg gcaatttgct gatcgctgcc
    16801 ggatactgct actgcctaat cgcctcaagc aaggcattga gcatcagata ggacgaggtg
    16861 gcgcccgggt cctggtggcc aatgcttcgc tcgcccaggt agctggcgcg ccccttgcgg
    16921 gcctgtagcg gaatggtgtc cttcatgccc tgttcggccg cggcaacggc gtcttccatc
    16981 gcgccgacat tgcctttgcc cgcagcgatg cccgctctca acgcggccat cgccggtaac
    17041 agggcgtcga tcatcgtctt gtcccccggc tgcgcccgcc cccgcgccag aacgccgtcg
    17101 actgcgccct ggagcatggt taatagatct tcatcattca actcttcctt ggcgtcggcg
    17161 gccatgccgc cgcgctggaa gaatgtgccg tagagaggcc cgctggctcc gccgacgctg
    17221 gagatgaggg tcatgcccac agttttcagg atgttgccga tgtccttgtc ggccacggtc
    17281 ggcagcttct cggccacttt ccgaaagccg cgatccatgt tggtgccgtg gtcggcgtca
    17341 ccgattgccg agtcgagttg ggtcaggtaa tccttgttct cttcaagaac cgcggccgtc
    17401 aactccagcc agcgaacaat ttgctctttg gtaacgttag tcatgcgggt ctctttccaa
    17461 atcaggaatt gaggatcagg aattatccca atcctcaatt cctgattcgt aattcctgat
    17521 tcctaattac tcagacgccc cagcgcagcc ccggcgtctt caccggcgcg tcccagaact
    17581 tcaatatctc atcatcgacc ttcatcaagg tgatcgacac accggccatc tccagcgagg
    17641 tgatataggg gccgatcagg ttgcgcacga tctcgatgcc cttggccttg cagacttcgt
    17701 ccagcttgcg atagacggcg tacagctcgg atacgggcgt gccgcccatg ctgttgacga
    17761 aggcgatgac cttgtcgccc cgtttgaacg gctcatcggt gagcgacttg tccacccagt
    17821 cgccggcgtc gcgatcccac tcgcggacca cgcgactgta ggcggggtcg tcgatgatct
    17881 ccagcgccat catctcggtg atgacgtcgg ccggcgccat cggcatgcgc ttctggcccg
    17941 gctcgccgtg gataccgatg ccgaactcca tctcgttctc gcccagctcg aaggtgggtt
    18001 tgccggcggc gggtacggtg caggaagtca gggctatgcc gatgctgcgg ccgtactggt
    18061 tggccttgcg ggccaggtcg gcgcactgat cgaggtcata accggcctcg gcggccgcgc
    18121 cgacgatctt ctcaacgaga accgtcgtgc ccacgccacg gcgaccggct gtataaaggc
    18181 tgtccttcac ggctacgtcg tcgtcgatca ggatattctg caccgggatg ccgtcgccgt
    18241 aggccagctc ggccgccgtc tcgaagttga gcacgtcgcc ggtgtagttc ttgaccagga
    18301 acagcacacc cgcgccgctg ttgacggctc tggctgcctc gtacatctgg tcgggtgtgg
    18361 gtgaggtgaa tacttcgccg gggcaagccg cgtcgagcat gcccatgccg acgaaaccgc
    18421 cgtgcatcgg ctcgtggccg ctgccgccgc cggagataat cgccaccttg ccggccaccg
    18481 gcgcgtcgac gcgataaaca tagttcggct cgaaatggac ctttaattcg tcgggatggg
    18541 ccagcgccat cccctcaagt tgttccttga cgacatccgg aaccgcattg atcagttttt
    18601 tcatctttcg gactccttgc cgcagcgcac aaacaattaa ataagccggg tgcttgccaa
    18661 atttgatccg gccttcatga agccaatcgg ggcgacccgg ctctccaaat caaaagaggt
    18721 agccgatttg gcgcgtcggg ggatggacat cgcggggcag gtaaatccgc gatgtccatc
    18781 tccggacaat gcctaaaaac gaccttgtct catggcgttg attctagaag aacatattcc
    18841 acatgatagc gccaatcacg ccaccgacga gcgggcctac gaccggaatc cagccatatc
    18901 cccaaccgga aggacccttc ccggggatgg gcagaatctg atgggcaata cgagggccga
    18961 ggtcacgggc ggggttgatg gcgtagcccg tcgggccacc gagggagagg ccgatgcccc
    19021 agaccagcat gccgacaaga taggggccaa gaccggcggc cagaccgtct ggggcgacgg
    19081 ctttactgaa gatggcgccg acaccgaaca gcaggacgat cgtgcccacc acttcagtga
    19141 cgacgttcca caacgggttg gaaatggccg ggccggtcga gaagacggcc agcttgaggc
    19201 ccgggtcggg cgtcgcgccc cagtgattga aataggacaa ccagacgaca accgcgccga
    19261 ggaaggcgcc gataagctgg gccaccatcg tcatgatggc ctcgctggcg gaatagacgc
    19321 cgaggatcag ccatttgccg atcgtgacgg ccgggttaat atcggcctct ggagcacccg
    19381 cggaaatcgc aataaacaca ccaataacaa ccgccatggc ccagcctgtg gttatgacga
    19441 tccagccgga gccgttaccc ttggttttgg ccaggacgac gttggcgacc acaccatcac
    19501 caagaatgat caggaacatg gttccgagta attcacccat aaaattagat ggcactttct
    19561 ttctccttgt caggttttca tgtccgcaga atggccgatc tattcccgta aatcgcgttc
    19621 catttctgcc ggaatttgaa cggaagtcgc tttgtcaggg gggatgtatt cggggtatct
    19681 tctctcgata gaaccaactc cttgtccagg cggctacatc ttcacgcgca tgtatttaga
    19741 tgacacaaac aactgatgat gaagatgacg tgaccgacgc ccccatggac gaactacccg
    19801 gacgctgctg acggacgaac ctgttgaccc aatggtatgc acaattgcca actgcaggca
    19861 gtatccattt gtgcaaaaca gataacctac atcataaaag gctaaaatga tgaggtcaag
    19921 cgaaacgcaa aaaatgtttc ttttcgaaac aaataggtaa tttgtatgtt tcagaacgaa
    19981 acggtctatt tcttgaacgt tttgggcgag atatccagtt gcttcatctt ccgccagagg
    20041 gtcgaccggc cgatgcccag tgttttcgcc atctccgtca ctcggccgtt gcaggcccag
    20101 cccgcgcgca atatcgcgtc gcgctcggcc tcgtcgaccg agatcaccgg ctgggccaac
    20161 gggtggcgac cggaaagcac gcgcccgttg cgcaccatct ccggcagatc gctggggcga
    20221 atgatcccct cacgcgcctg attcatggcc cgttccagcg cgctttccag ctcgcgcacg
    20281 ttgcccggcc aggggtagcg cgagaggaaa cgcacggcct catcctcgat ccacaccgcc
    20341 cgcccgtcgc gcgtcgtttg ccgggtcagg aagcgctcag ccagcagggg gatatcctcg
    20401 acgcgctcgc gcagcggggg gacatcgagc ctgaacacgc cgaagcggta gaacagatga
    20461 ggcatgaagc tgccctggcc caccagatct tccaggttgg ccatggtgga tgcgatgatg
    20521 cgcacgtcga ccgggatggg cattgagccg cccagccggg tcacatgctg ggtctcgatg
    20581 acatgcagca ccgccgcctg aatctccaat gacagattct cgatctggtc gagcagcaag
    20641 gtgccgccgt cggccagctc aaacttgctg ggttggccgc gtgtgccggt gtcctgttcg
    20701 gcccccagaa tctcgcgaac catcaattcg tggggaatgg cccggcagtt gatgtcgata
    20761 aatggcttgc tggcccgggg gccgtcgttg tggatggcgc gagccagccg atttttgcct
    20821 gcgccgccct cgcccatgat gagcacgggg gcggtgccgc gggcggccgt gcgtgcctgt
    20881 cgcactacac gacgcatgcc gggcgaatcc gcttgcacgt cctcgatgct cagtccggtc
    20941 tgcgccccgt attgctgttg ggcgatctgt cgaacgtgct cgatcgggct gagcaaggcc
    21001 acgtagctga ccggttgtgg gccggcctca tagatcggtc gcagcgtgat gacggcgcgc
    21061 acgtcccggc cgtccgcctg caaacttaac tcaacatcgt gcagttcaga cccggtctca
    21121 atggcggcgg tcagctcggg cggcagccgc agcacgtcac ccaggggtcg ccccagcaca
    21181 ttacgcgggt tgatctggag gatatgggcg gcctgctcgt tgacatggtg gatctccccc
    21241 tcctgattcc agacgatgac cccttcggta atggccccca gaatcgtgtt gacctggctc
    21301 agatgctggt tgacctcttc cagataccaa ttggcctgaa attggttgcc gatggcccgc
    21361 gcggccgaca tcaccaggcc gagcgtatgg aaagagggcg actccagcgc cccggccatg
    21421 cccagcaagc cgataatccg gccatgcacg tcgtggatgg gcgcggcggc ggtgaccgtc
    21481 tgatggagaa aatcaaaata atgctcggcg ccgatcacct gcgtgggcat ggcttccatc
    21541 aataccaggc cgagggcatt ggtgccggcg ttgttttccg accagtaggt cccctgatgg
    21601 aaacccagcg cggttgcctg tgccatagcc tttgggtcgc cgccaatcag caggatgcag
    21661 cccgcgccgt cggccagcag gatggcgtag ttcgatcctt cggtgaattg atgaatgtct
    21721 tcgatgaagg gggtggcgac ggtgatcagg tccagttggg cgcgcatgat ggagctgagc
    21781 gcgccggcat tgatcggcgc cacgcgcggc cgcgccaagg ggttgaagcg attgacgcag
    21841 cgccgccacg aacgaacgat ggcggggtcc atgcgcgccg cctcgatcgc gtcaagtcgc
    21901 ccggtctcgg tgaacgagag ccaggactcg cggattgcct cgtttcgggt gccgatgtct
    21961 gccggtagca tggttatcaa gaaatggcga tgtctgtcgt cgcgatcctg tcactcggtg
    22021 atgacagaaa gcctgcgatc acctcaacga catgtcgcgg atccatagcg cctaactata
    22081 acgcctcccc ggcaaagtcg gtagattctg ccgcatccat tcgtttggat gacccgccgg
    22141 atagacgtac aatccatgat cctggcgaac atttccgccg cgttgacggt tacctgctta
    22201 acccaaagtt aaatacttat atggttccta tggaatgctg gtaagctacc attatataat
    22261 gaaagatgtc gaatcaatga ccgtagacac cgaccggccg gcgccctcat tatacgattg
    22321 ccgaaattgg ctcggctcgc gccgactcac tttaatacga aaaaaggccg gttgccttgc
    22381 gggagtctta taaattatgg atgaacaacc gaacgatagc cgaccctcga ttccaacatg
    22441 ggtctggatc atcaccatct tcgcggtgat attggggctg cagctctggc tgagcggcaa
    22501 atttagcggg ccggagcaaa tctcgctcca gaaaatggcc gaccacatcg ccgccggcga
    22561 tgtgaaacag ttggtcgtct ctggcgaccg cttgcaggtg acgcttaagg acgatcgcac
    22621 ctttggcgcg atcaaatctc cttccgacag tctgatcgag accttcgaat attacggcat
    22681 ctcggccgaa caattaaaca acaacctggt cattcgcgat caatcgacgt ggaataccct
    22741 gatcaccatt gcattgagcc tcggcccggt gctgctgctg atctggatct tcacgcgcgg
    22801 ctttcgccag atgcagggcg gcggcggcaa caacatcttc ggcttcggcc gcagccgcgc
    22861 ccgcaatctg accgacgcca accggccgac cgtcaccttc gacgatgtgg ccggggtcga
    22921 ggaagccaag ctggagctgg ccgaggtggt gcaattcctg cgcgaaccgg agaaattcgt
    22981 gcaggtcggc gcgcgcattc ccaagggcgt cctcatggtc ggcccgccgg gaacgggcaa
    23041 gaccttgctg gctcgcgccg tggccggcga agcgggcgtg ccgttcttcc acatctccgg
    23101 ctcggagttc gtggagatgt tcgttggcgt gggcgccagc cgcgtgcgcg acctgttcga
    23161 gaaggccaag gccgccgcgc cctccatcgt cttcgtggac gagatcgacg ctgtcggccg
    23221 ccagcgtggt gcgggcctcg gcggcggcca cgatgagcgc gagcagacgc tcaaccagat
    23281 tctcgtcgag atggacggct tcgataacga gaccaatgtc atcgtcatcg ccgccaccaa
    23341 ccgggccgac atcctcgacc cggctctgct gcgccccggt cgcttcgacc gcaaggtctt
    23401 cgtcgatttg cccgacattg ccggacgcga gaaaatcctg caggtccacg cccgcggcaa
    23461 accgatcgcc ggcgaagtga acttcaagga agtcgcccgc ctgacggccg gcttctccgg
    23521 cgccgatctg gagaacctca tcaacgaggc ggcgatcttc gccgctcgac gcagcaagcg
    23581 caccatcggc ctgctggagt tccaggacgc cttcgaccgc gtggtcatgg ggccggaacg
    23641 ccagacccgc gtgatgagcg aagacgacaa gatgacggtg gcctatcatg aggcgggaca
    23701 tgccgtcgtc agcttcttcc tgactcacac cgacccggtg cagaaaatca ccatcgtgcc
    23761 gcgcggccgc gccggcggct acgtcatgtc attgcccgaa gaccgcgccc tctatagtcg
    23821 cgagttcttc gatgaccaga tcgccatggc tctgggcggc cgggcatcgg aggagcattt
    23881 cttcggccgc gtcacaaccg gcgcatccaa cgacctgcag aatgctacgc gattggctcg
    23941 cgccatggtc atggaatacg gcatgtcgga caggctcggc ttgcccacct acggcggcgg
    24001 ctccggcagt ccgttcgtcg gccgcgagat gggctttttc ggcagcggcc gcgactatag
    24061 cgaggaagcc gctcaggcca tcgacgccga ggtcaagcgc atcctggaag agaactatgc
    24121 ccgcgcgctg ggggtcatcg agcagaagca tgatcgcatg gtgcaactgg cgacgacgct
    24181 tatggacgtg gagacgctcg accggcccgc gttcgagaaa ctcatgaacg acccgatatc
    24241 ggataatggc cgggtccctg atgaggttgt ggccgacgcg cagcccgtcg aagcaattta
    24301 ggcgtttgga tgcagggggc aggtgcgaca ggcagcctgt cgtccaaacc cgcccctaca
    24361 cattggcggg tgggttgcaa gcccgttcca atcagccgca ggactcgcaa tgaccgaaat
    24421 gacccaggcc gtcgtaactt cccgggacgg cagcgtgctg acggtatccc tcaaccggcc
    24481 ggagcagcac aacgccatga accccgccat catgcatgag ctaatgcgca ttttcggcga
    24541 gctgccggcg cgggatgacg tgcgcgtggt ggttctgacc ggcaacggcc gctctttctg
    24601 tgccggggcc gacctggcct tcatgcgggc cgcggccgat tacaccttcg agcagaacgt
    24661 ggccgacggg caggcgattt tcaatctgat gatggctgtc gactcctgcc cacagccggt
    24721 cgtcggccga atcaacggcg cggccatcgg cggcggggcg gggctggtca gctgttgcga
    24781 tatcgtcgtc gcttccgagc gagcgacctt cgccttcagc gaggcgcggc tgggcatcgc
    24841 tccggccgtc atctccccct tcgtcctgtc gcgcatcggc cccggccacg cccgcgaact
    24901 tttcctgacc ggcgaacggt tcgacgtccg ccgcgcccag gctatcggcc tcgtccatca
    24961 tgtggcggcc gagagcgaac tcgacgccgt cgtggccgag cgggtgaccc aattgctgca
    25021 agccgccccc ggcgcgcaag ccgccatcaa ggagcttatc cgcgccgtgg cccatcaacc
    25081 caaggccgat gcgcgcgaat acacggccga aacgatcgcc gctcgccgtg ccagtgacga
    25141 aggccgtgaa gggatgagcg cattcctcga aaagcgcaag ccgcgatggc aggaataatc
    25201 aggaattagg gattgcgaat tagggatggt gcgcattcct gattcgcgac ctgttgttta
    25261 tgaagctggc tattctctcc gacatccacg gcaattggcc cgcgctggag gccgtcgcgg
    25321 ccgatatcga cgcgtggcgg cccgacaggg tactggtcaa cggggatgta gtcaacgatg
    25381 ggccaagcag cgtcgcttgc tggcgctacg tggcccgacg acggcatgac gacggctggc
    25441 aagtgctgcg cggcaaccac gaggaatacg tcaccgaatg gtgcgacccg tccacaccgc
    25501 gccaaggccc cgcctatgag ctgattcgcc tcagtcaatg gacgtatgag cagtttggcg
    25561 gagacgtagc cgagctggcc gaactgcccg accgctggga atgggccgct cccgacggca
    25621 cggcgctgat cgccatgcac ggcacattgc ccggcaatcg cgtcggcatc tacccattca
    25681 cgcccgatga gatcatccgg caacgaatcc ccaccgccgc ggccgtgttc gtgaccgccc
    25741 acacccacat agcccacata cgccaactgg atagcacgct ggtcgtcaat atcggctcgg
    25801 ccggcatccc cggcgacggc gacggccgcg ctgcctatgg ccggttcacg tgggaacagg
    25861 gttgcggctg gcaggcagag atccggcgtg tcgactacga tcgatcggcg gccgagcgcg
    25921 attacttcac ctccggcttc atgaccggcg tgggaccgga agcggaactg tcgctggtgc
    25981 aattccgcat ggcgcgcgac gtgcgcacgc gctggtcggc caactatcgc gagcgcatcc
    26041 tcaatgggga gatgtcggtc atggaatcgg ttgaccaatg gttgcgccgc gacgaatttc
    26101 gcggctatcg cctggccgac gcgcccgcgc cgcgaccggc tgtgtgctaa tcaggcgctc
    26161 ataaaccggc gtatcagaga caaaatagcg acactttggg ggcattcatg ggctttccgc
    26221 ggttataaac aggcttgggg cttgcggaat ttcagccgct tttctataat gtgcgtggat
    26281 ctggaagagg aatttgtttg aattacattg atctgttgtt gtctggaacc atattgccct
    26341 ggctgctagg gttactgctg gcgcttggcc tcgtggctct cctgatgtcc atgaagctgt
    26401 ggcgtgatac caagcggtcg ccctacttct ttatgcgcca gcaggccgcc cgacggatgc
    26461 aatcctatct gtcggcattt gtcgccctca tgctgttggc ggtcggcatc ggcgtttatg
    26521 ggtggcgcac ccctgaagat gccacggtgc gcatggccct catcacccgc gccaagccgg
    26581 cgaccgccga agtccaggag ttgatggccc agccgtcggt tgccgagcgc gccacggccg
    26641 ccgccgtagc gactacaacc cgcagtggcg gcgtggccaa cgtcttgctc agcgcggacg
    26701 aagccacctc ccttgtccct gaacttcccg ccgcctatga tcaggttaaa tcactcgttg
    26761 atctgaaatc atccactgaa ctcggcaccg tcgttctttc cagcaccgtc accgacgaat
    26821 acaaagccat caaccccgcc agtgtcttcg gcgaggggtt ctacacgctc tatgccacct
    26881 tcgcctataa ggacatggcg gacggcatgg cctggtcatg ggtgtggcga cacaacgggc
    26941 aggtcatcga cggtggcaac gagctttggg cctatggcga tgaaggcccc ggctatattt
    27001 atctgaaccc cgaagagggc tttgccgaag gtcgctattc gcttgaagtc tgggtcaatg
    27061 gcgaactgtt gggccaggcc tcagccacca tgaacgacgc ggccgttgcc gctggaaact
    27121 aaacatcgcg cttgtccgaa cagcgaaaga aggcccacgg ggcctttttt gttatccggc
    27181 catccccgcc gctattatcc aaaccaatcc tatgatcgac ccggcaaacg acgcgccaga
    27241 atgccgacga accgctgcca ctcctcgacg cgcagcagtt ggctgatcag cagataggcg
    27301 acgcctccgg cggccgtgcc gatgatggcg cgtgaccaga cggccgaagc cggcagccac
    27361 agcagcaccc cggcgacggc cgcggccatg gccagcgatg ccacccccat ccgcgccagg
    27421 cccgacagca gggaacgacc attcagccca ttcagcttgc cccgtatcag ccacaacaac
    27481 agacccactt caagcaggtt ggacaggctg aaacccaggg ccaggccgcc caacggcaac
    27541 cagcccagcg tcggaaa
//
LOCUS       DAOMQG010000009        27347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_762_length_27347_cov_12.419170, whole genome shotgun sequence.
ACCESSION   DAOMQG010000009 DAOMQG010000000
VERSION     DAOMQG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 27347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27347
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_762_length_27347_cov_12.419170"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2240)
                     /locus_tag="PLC06_01295"
     CDS             complement(<1..2240)
                     /locus_tag="PLC06_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRO89108.1"
                     /translation="MRPNKRPLIYLLLVALLLLVGLLVACSNQGGQTSGGGQGTGASQ
                     PTAGAVAEATAAVSEESGGTGAVTEVEVTRIVEVPVESAPSVAVPFYDEWANSPHNDT
                     EGEPFNHWNEEDPAEVPTDCAKCHSTPGYIDHLGADGSTPGVVDAPAPIGTTIECTAC
                     HNQVTLTKTSVVFPSGIEITNLGDEARCMECHQGRASTISVDQRIADAGLTEKVDATS
                     EDLGFTNIHYYAAAATQFGTLAKGGYEYMGQPYDARFDHVPTYNTCIECHDMHTLEIK
                     VSECQACHTDVKTTEDLVNIRMNGSLVDYDGDGDMKEGIYYEIEGLREKLYGALQMYS
                     VETTGSMIGFSDTAYPYFFIDTDMDGTLSEAEAVYPNAFNAWTPRLAKSAYNYVVSKK
                     DPGAYAHGGKYIIQLLYDSITDLNEAVDAPVDLSAANRIDHGHFAGSEEAFRHWDAEG
                     GIVPGSCAKCHSATGQPEFIAEGVVTSQPAGNGLTCGTCHNDVQNFTLYDVPAVRFPS
                     GATVSFGERDSAANMCMNCHQGRESTVSVNRLIGDLPGDEVSEGLRFLNIHYFAAGAT
                     LFGTEVKGAYEFAGKEYTGFNEHVGTFNSCTECHDTHALQIVIKDCGECHEGADTLEG
                     LRTIRMTAVDYDGDGDVTEGVYGEIDTMRERLYAAIQAYAIDVVGTPILYDSHAYPYW
                     FADTDGNGRVNDEEGGFSTWTPNLLRAAYNYQDSHKDPGGFAHNSSYVLQILFDSIQA
                     VGGDLTG"
     gene            complement(2339..3442)
                     /locus_tag="PLC06_01300"
     CDS             complement(2339..3442)
                     /locus_tag="PLC06_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRO89109.1"
                     /translation="MDDSSIEPEAIAPETKVPEGAAPEAPAAPEAPVMPPAPEVLAAS
                     VETPPVPVTPGELPADEVAHEVAHEVAHEVAHDPGAAVMTPHGPEYPRFRLMARIEHM
                     TLLISFTILCLTGLPQKFPFSPVSQGLIQIIGGIEVARMIHRWAAVVLILGTVYHLLT
                     SAYRLFVKREDMRMLPELKDGYDLRDTVAYNLGFIDEPPRMHKFNFGEKFEYWAVVWG
                     TGVMIFTGFILWNPLAATQIIPGDLIPVALEAHGWEAVLAAVSIVIWHLYNVLVKHRN
                     VSMFTGTLSHKIMEEEHALELERLEAGGAPWKVVPPKILARRRRIYFAVASVVTVLAL
                     ALVIWMFTFEDTAIIMEPAATRGVFVPLATPYP"
     gene            complement(3459..5153)
                     /locus_tag="PLC06_01305"
     CDS             complement(3459..5153)
                     /locus_tag="PLC06_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRO89110.1"
                     /translation="MRQVSKVGPRVVAPVLIAFLVALSFFANMESARAEQPVAPSGAP
                     PVGPITILLPSLDQPAQTGQPAIHPTTDFACHSCHSDTDAVVEFPSGESLPVEVDLRS
                     FDASIHGADSGAFIGCSGCHAPARYQFPHPSPEAGTLREYTILQSQSCVNCHDPHLTA
                     HPGPEWSGGYDVAASESGLPVVCTDCHGDHHVQTAKAWRLPTSTTVCVDCHTGAGIAL
                     TDPGSLSRHIEAGLFAQKQINNDFCMGCHGPPNKTMTFPNGDEISISIDGEGLHASVH
                     GIGNSWQALNCTDCHQDYDYPHPPLQAETARDYTIQQSELCARCHEPQHEGQMSSSHA
                     QALADGNQDAATCVDCHSSHYTPPPSEPRSRISETCRQCHSAIFDEYAESVHGAALLG
                     DEDPNVPTCIECHGVHDVGDPTTPLFRNRSPELCASCHANEEIMSQYDISTDVFDTYV
                     DDFHGTTVTIFQSSDPNVATNKAVCYDCHGVHDIRRPNDPDSGIKENLLETCQQCHPG
                     ATANFSNSWTGHHRPSLRENPLMLLVAIFYAMVIPGTVLFLTFLVGTDVYRQMRGR"
     gene            complement(5250..6482)
                     /locus_tag="PLC06_01310"
     CDS             complement(5250..6482)
                     /locus_tag="PLC06_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRO89111.1"
                     /translation="MNVGRTAIWLLLATLVLAVAACSGRPGPAGPQGSQGLQGPPGPT
                     GPQGLAGARGPAGQDGVSFTPAGFIGSAACANCHQDTFNTFAQSGHARALTPVVNGEA
                     PAFLPSTARRTPPEGLAWSDIAYVVGGFNWKAQFVKTDGSLVTGEAAQFNLDNNQLEA
                     GNEYVAYHAGEEKPFDCGACHTTGYSGRESMPGMPNMVGGFVEPGVQCEACHGPGSLH
                     VNNPRSFALKVSRDNQECRACHMSGELVTADGFIQHTGHEYNDLFPGKHRLIDCVDCH
                     DPHTAVVSPRADHEPFLEATCDGCHFQQAQVEKVHLRIRVACVDCHMPQLIQNAIGRP
                     ESFMADMMTHQVVINPTQMDQFAADGTILPQIGLDYACRQCHNGELGIGPNLPDSALL
                     GAAQGYHEPEPEDLTGEQ"
     gene            6844..9375
                     /gene="gyrA"
                     /locus_tag="PLC06_01315"
     CDS             6844..9375
                     /gene="gyrA"
                     /locus_tag="PLC06_01315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRO89112.1"
                     /translation="METGTINSIDINQEMRSSYLDYAMSVIVSRALPDARDGLKPVHR
                     RILYAMYDMGLRPNTPHRKSARIVGEVLGKYHPHGDSSVYDAMVRMAQEFSMRYMLVD
                     GQGNFGSVDGDSPAAMRYTEARLASIAMELLEDLDKDTVDFTTNFDDSLEEPSLLPAR
                     LPNLLLNGASGIAVGMATNIPPHNLGELCDAITYLIDRQDDIDDVTLDELMEFVKGPD
                     FPTGGVIIGNEGIRNAYATGRGRVVMRGVANIEPNPRHPDRMQINITELPYQVNKAML
                     IERMAELVTKGVIDDISDLRDSSDRNGISIIVELKRHTQPRKVLNQLFKYTALQSTFG
                     VQLLALVDKQPRLLSLKRALQIHIDHRVDVITRRTRFELDKAERRQHILAGLLIAIGN
                     LDEVIQTIRQAADADDARSQLMTRFGLTEIQATAILDMQLRRLAALERQKIEDEYAEI
                     TAHIAYLQSLLQDHGKILQLIKEDLAKLKEKYGDPRRTQILPGMDEEFNIEDLIKDED
                     VFISITRRGYIKRTPVSIYRRQSRGGKGLIGMTTRAEDELEHFFVAGTLNTMLFFSDL
                     GRVYATKAYEIPELDRTAKGVSLMNILPLSQEEKITAVLPVHDFEAGKYLIMITRKGR
                     IKRVELGMFANVRPSGLIAINLETGDSLRWVKMTQGNEDIILVSQQAKGIRFNEENVR
                     PMGRTAVGVNAMRLDTWDQLAGADVASSDDDLLIITEKGYGKRTPLEEYRQQGRYGQG
                     VRAMNLSPERTGAIVAARVVGPKDEVTLISSGGILLRMSAAHISQQGRASQGVRVMDV
                     RDGDTVASVAVIRETRQAVTDAEMNGDDGQVTNDERRVTNEERSS"
     gene            9889..10794
                     /locus_tag="PLC06_01320"
     CDS             9889..10794
                     /locus_tag="PLC06_01320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRO89113.1"
                     /translation="MEDLLQWADYQTELGGMFQGKFEEWIQRPEFELLNGKIQLIFTS
                     PPFALNNKKRYDNHQGEAYKHWLASYAPIFRRLLTDSGSLVIEMGNAWEPRLPVMSTL
                     ALEALLDLKRAGDFYLCEQFVVHNPARLPSPAQWVTINRNRVTDSFTHIWWLSNTPYP
                     KADNRSVLRPYSKAMLRLLERGTYNDGKRPSEHVVGAESFLKNNGGSIPKNVLEFSNT
                     SSSDPYQKYCKENGILVHPARMNIGIPNFFIKFLTDSNDIVLDPFAGSNTTGSSAEAL
                     GRSWLAFEPVADYVAGSRGRFSGLD"
     gene            10798..11991
                     /locus_tag="PLC06_01325"
     CDS             10798..11991
                     /locus_tag="PLC06_01325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRO89114.1"
                     /translation="MKQNFELRTWQKEAFSIWERQMRGVVAAVTGAGKTSFALLCIEK
                     FFRTIPNGVVLVVVPTQALLDQWYLSLVDDAGVQSSDIGVVASGRKDWRGRKYVISVI
                     NSARALAHKISEQAPTMLVVDECHRAGSEKNAQIFTGSFSATLGLSATPERQYDDGFK
                     RLIQPNLGDIIYRYGYERAYADGVIAPFKLTNIRFSLTQQEQEEYDMLSRRIAVLLQN
                     TNEDSGERIKLMLLKRSRISWNSPLRVPLAARIALEHQRDRVIVFHESVKQADEIYRI
                     LRARNLRTTIYHTGMSPEHRRENLRLYRNGYYTSLICCRALDEGLNVPLTSVGIIASG
                     TSSMRQRIQRLGRVLRQIPGKESAIIYTLYATDSEANRLMHEADKMVEITSVKWLGVN
                     LEFSG"
     gene            11984..12790
                     /locus_tag="PLC06_01330"
     CDS             11984..12790
                     /locus_tag="PLC06_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89115.1"
                     /translation="MAKIIHEGNVYQEVSLTMFHEREVQDQLASRANLVFPGYIYAEF
                     MPLVTTGTDSAKPDFILVENEYRSWVVGEVEMVRHDLTNHVLRQVQVFLNGEYGEKHV
                     HHALHQCSSLNEKRLRLLIESISPSVLVVADRPDVAWKTVINGQGAKMAVFEVFRRHN
                     SADLIFRVNGDIPEVHGDFITTLKFDRSLRRLLRINTPSGLAAENGNKIEIEYNGMIT
                     EWVVMIAEDKAWLNPVGHPDMPDDATFMLGTTSEGYLRMEVAKNAAHNRS"
     gene            12768..14273
                     /locus_tag="PLC06_01335"
     CDS             12768..14273
                     /locus_tag="PLC06_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89116.1"
                     /translation="MQLTIGLEAINSYKRLSYSTWHALAEFVDNSTQSYFDNRTILDE
                     AFRKEESKLTVSILYDRDSKTITIKDNSIGMSETDLEEALKIASRPPRSNSRSIYGMG
                     LKTAACWFGDRWTVTTKKLGEPNVISVTVDVPATAAGNGNLTPIRKEADIDQHYTEIT
                     ISHLHRGINGPAVKIIKEYLCSIYRCDLRENVLELLFQGKPLKWEGFEDRILRDDQGQ
                     LYKRDLDFYVLGKRVTGWVAVLESGGRPYGGFSVLQNKRVIKGYPKGWRPRKIFGQEE
                     GSNDLINQRVCGELFLEDFQVSHTKDDIVWESDEEDMIESELFIRSEDYRRVATKQRK
                     GDQRGPSQLALDNAVNQVFKELSSDEMIDVIRLTEIPPESILKQTSQMTIQHAAKGKP
                     RFDVELGDMRVRFFVDSDASPRDTYYALDWKPDGTIEIAINRNHDYMVQLGNDVADFI
                     RQCVYDGISEFKATQKRGRVEPNTIRFIKDNLLRVPYRIHDDTYEGDDDLE"
     gene            14654..17473
                     /locus_tag="PLC06_01340"
     CDS             14654..17473
                     /locus_tag="PLC06_01340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89117.1"
                     /translation="MTRLTRYHALAFMVLALLVAAALRLPDLSAIPPGVHYDEAAYGL
                     NAGDIGLRGDRPIFIPAFTGREPLYLYVAGGMARVLGNTIFALRLTSAFFGLLTIAAT
                     YWLGREMLADRRIALLAAALLAISFWHVLFSRLGFRVISQPLLQALAVAALFRGLRGG
                     RWPWLWFAGLLVGLSAYTYLAARLFPVLLAFALLPLLLDAGTRPVRLRQLAFVALVAF
                     LTALPLLAYFYFNPDAFWVRIGQVAPGESGLSLTESLARSFAMFFLRGDPYLRFNLPG
                     RPLFDFVTGGLLLVGWLFGLLRYRRFPYDWQRAAVLLLLLAPLVMILPTALATNEIVP
                     SNLRAMGLIPFIFFLPPIGLIALLRDVERRFGRPPLTRAVSWVALFILLAGGTYTQYL
                     YFNHWATQDELLLVNDGDLTAAAGWLDDTLAATPLAERPAVYVAAVHYRHPTMAFLSA
                     NYDSFNWLPGSHALVFPASGPALYLYPANSPAPAWALPYLEAEGPPEVVTAENGVELF
                     RVYRLSAPPTLPAATEPANFGGLIALDGVITEPAAGGESLPVTLRWTVTAASPAGVAY
                     STFVHLEDRNGHRWSQIDQDGYPAGQWRPGDVILERLDVAVPPGMPPGEDGVYRLRIG
                     RFDPADGERLPRLAPNGAFGGNSLLSDDVTIRHAAPPNPLPEPPFPINEAVIDGLRFL
                     GHEPIARAAETGETVPLALWWAAEQPLPSLAINLSLVGPDGIAHPFGWTQPAYGTYPF
                     SLWETPAFVIDRQDVRIPDELPSGEYRWRLELAQGDKLVYLVDLGSLRVTQAERLFEL
                     PPFATAMGATFGEEIALQGYTLETGFPGRTPVSNSIPLELVWQAVAQPTNDYTVFVHV
                     LNADGTCCAWQSDVMPHGGTYPTSRWWPGEVVVDSYEIVLPEGASIEDHGIEVGLYLA
                     ETGQRLGVMVDGEATGDAVRPGSGQ"
     gene            17531..18190
                     /locus_tag="PLC06_01345"
     CDS             17531..18190
                     /locus_tag="PLC06_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRO89118.1"
                     /translation="MKRTAILFDMDGLMVDTEPLARRAWERTVALYGASIDDDLYNQT
                     IGRRTVETTQLVLDALRLPVAHADLMRRKTDEYMNILAGGVPVMPGLWTLLDRIDAAG
                     LPWAVATSTPRPVAEIVLGMLGVTERYHALACGDEVTNGKPAPDIFLLAASRLGALPA
                     ACLALEDSPAGCTAAAAAGMRVAAVPTDMTRSEAFPCAYRCYPSLAEVAADLEQLLAA
                     D"
     gene            complement(18187..19347)
                     /locus_tag="PLC06_01350"
     CDS             complement(18187..19347)
                     /locus_tag="PLC06_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89119.1"
                     /translation="MAEPRPIDRIELDLSAEDENAARTYRRLSTLNGLLIGLAVGLGA
                     WGMDVLQIARLPMASYLPLLLAGIGLTTAMGAAIGWLTGRIARTPVTILLWSLAGMAF
                     MLILSYLPYQGRTLLVWLTDSRFRGRDIYPFTLEATVVGLLLGGFFITLALAGLGLVQ
                     NYRLEQSLLHIGQRGGLSARGWASLLLPLPIVFLASMVTNNTMANPAASAARLVHQVI
                     PIARDYDGDLRLLDLGDGISYAALRPVHELLRGGDYRLSIVDVNPLMATVVIGVDIPD
                     TGAWIYCRVVNDQLNFCYDASIPYVLGLHSLVTGMPPPEDCRDCILPTTHEAEAWLDE
                     HRARFGLDPVIERLARQGSHVLMQITGDDLVAECWIEGITSMQLMECGEVRN"
     gene            complement(19326..20066)
                     /locus_tag="PLC06_01355"
     CDS             complement(19326..20066)
                     /locus_tag="PLC06_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89120.1"
                     /translation="MLRRFAAFGAALIMAGALWLFLYGMTMAQIERGGPASTPTPDAG
                     PPPTLAPGVMPTVDRLAPPPTVVSPNQADEGAYLFWLHCMPCHGDQGQGLTDEFRAQY
                     PHEDQNCWNSGCHGERPYGQGFQLPTAVPAIIGDGTLQKFDTMDAVYRYASVAMPYFY
                     PGSLTQEEYLAILAHIAREDGLWDGTELTIDNLTDLQLRPEAAVSSPTVAADAPRAGG
                     IDAATFTIFGIIIVALIGGVHLWRNRGR"
     gene            complement(20249..21307)
                     /locus_tag="PLC06_01360"
     CDS             complement(20249..21307)
                     /locus_tag="PLC06_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89121.1"
                     /translation="MTATTTEPVDHGPVSLDGGELREQTRLEKAVGPETARLLRGIAT
                     NPLSVTGIIVIVLFALVALFAPVLAPPPKENWPRMQIPRDGFRADPQPPGSPWNKNAP
                     MTVPWWYRNIWPRQEEWVHLWGTTSGQYDIWYGTIWGTRTAFFASFVVTLASTLIGVT
                     IGALAGFYGGWVDEGLMRIVEIFIVFPSLTGALILASLLTPVFGRSVLPATVALVIFG
                     WTGYARQLRGDILATKQRDYVLAARASGAKDSHLMMKHVIPNAMFPTMVRISLDLGAV
                     VLSFAALSFLGIGLPEGYADWGQIVSFARSWILTLDEYWFIIVFPGVALLLYGLGWNL
                     IGDALRDILDPKLRGKKA"
     gene            complement(21328..22392)
                     /locus_tag="PLC06_01365"
     CDS             complement(21328..22392)
                     /locus_tag="PLC06_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89122.1"
                     /translation="MAAYIIRRLLILPIILLGVTLLIFIFLQALGPVERSALYVRDIP
                     KTDRQVEAIITRYGLDKPFYVQYWNWLVGIEDGSTGKRMGGLLRGDLGYSRTGREEVI
                     DVLVRRFPATLELALWSMVPVIGFGIWLGIKAALNHNRPVDQTLRIFAILGWSFPTFV
                     FALLLLLVFYAKLGWFQPGRLSIAFSQEVLRPGFTKYTGMMTFDSLLNLRFDIFIDAL
                     RHLIMPVVTLMVIQVALLLKVTRSAMLEEMRQDYVTTARSKGLPEKLVVNRHIRRNAL
                     IPVITVAGLTLAGLLNGVVITETVFDYPGIGSWAAAAAVSLDVLSMLGYALFTGVLIV
                     IANLIVDILYAVLDPRVRLS"
     assembly_gap    22577..22586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22684..24741)
                     /locus_tag="PLC06_01370"
     CDS             complement(22684..24741)
                     /locus_tag="PLC06_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89123.1"
                     /translation="MFTKNRIGLLMAALLVLALGLVACQPQTQTVEVTRVVTETITEE
                     GQEVVVTSVVTEVQEVVVTATPVPPAAIAAPDPTTYVSQTFGDMDTLDPALGYDTASN
                     TTIMHIYESLIFYDGLDPTKFVPALATEVPSIENGGISADGKTYTFHIREGVTFHDGA
                     TLTPSDVAYSFQRGLLQSDPNGPQWLFLEPILGYTSGDVTEGIQEGAFAGDPEGLKAG
                     ATPEELLATCELVKAAVVADDAAGTVTFNLAQSWGPLLATLAGTWGSVIDQDWAVAQG
                     SWDGDCATWQDHYAPGAENTPLGAVANGTGPYMLDHWTPGEEYVLTANENYWRTAETP
                     LFEGGPFGPPAIKTVIYRLVDEWGTRFAALQAGDAAVVAINTADRPQVDPLVGEACVY
                     QQGCSPTESPDAPLRRWTGIPGISRTDIFLTWNIAPDSPYIGSGKLDGNGIPPEFFSD
                     VNVRKAMATCFDYDTYIAEVQLGEGIRNNGPIPTGMLGYNPDGPTYNYNPEECAAYFE
                     AAWDGALPATGFRFQAAFNTGNTARQTIGEMLQSELGGINELYRVETIGLPWPTFLRS
                     FRVSQLPIAASGWQEDIHDPHNWIQPYTFGTYGGRQAMPQEIKDKYQELAGAAAAESD
                     PAKREQMYFAIQQEWYNDIPTVILAMQTGTRYEQRWVNGYSEELARVVPGSFVYTMSL
                     QGQ"
     gene            complement(24964..26016)
                     /locus_tag="PLC06_01375"
     CDS             complement(24964..26016)
                     /locus_tag="PLC06_01375"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRO89124.1"
                     /translation="MTENISNAEVQSQASSLAPTSSQALIQVRGLKKYFPIKRGVLRR
                     TVGHVRAVDGIDFDIYKGETLGLVGESGCGKSTAGRSILQLEVPTAGSVKFADRELTE
                     MGKSDLRRARRHMQMIFQDPYASLNPRMTVGSIIGEPLAIHGIGDAASRKERVQELLR
                     VVGLNPYFVNRYPHEFSGGQRQRIGVARALATNPPFIVADEPISALDVSIQAQVVNML
                     DDLKHELGLTYLFIAHDLSMVRYISDRVAVMYLGRIVELSSRDEVYDHPLHPYTQALL
                     SAIPVPDPDKEARRQRIILEGDVPSPVNPPSGCRFHPRCAYATEICREVDPEFRNMGT
                     AAVPHMVACHHAEKFM"
     gene            complement(26016..27071)
                     /locus_tag="PLC06_01380"
     CDS             complement(26016..27071)
                     /locus_tag="PLC06_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89125.1"
                     /translation="MTILLDVKNLTTRFYTEDGIVHAVNGISFDLDEGQSMAIVGESG
                     SGKSVSMKSIMGIIPSPPGRIEAGEVMFNGRDLIKLSPEEMRRIRGREMAMIFQDPMT
                     SLNPVLTVGMQMTEALVLHLGMKQEQADERAAELLTLVGVGNAADRLKDYPHQFSGGQ
                     RQRIGIAMALSCNPSLLIADEPTTALDVTIQAQIVDLVLRLKQQLGMAIIWITHDLGV
                     IAGMVDRIIVMYAGFIVENAPVRALYKETAHPYTVGLLESLPKVDATKKERLIPIKGL
                     PPDLLKEPTRCPFAPRCPYAIEHCWTENPPLMPVSPDHYSACWRADEVFAGLRIHDNE
                     QMDDINKEALATLKEVA"
BASE COUNT         5759 a         7793 c         8142 g         5643 t
ORIGIN      
        1 ccggtcaagt cgccgccgac cgcctggatg gagtcgaaca ggatctgcag gacgtaagac
       61 gagttgtggg caaagccacc cggatccttg tgggaatcct ggtagttata ggccgcgcgc
      121 aacaggttgg gcgtccatgt cgagaacccg ccctcttcgt cgttgacgcg gccgttgccg
      181 tccgtgtccg cgaaccagta gggataggcg tgcgaatcgt agaggatcgg cgtgccgacg
      241 acatcgatcg cgtaggcctg gatggccgca tacagccttt cacgcatcgt gtcgatctcg
      301 ccatagacgc cttcggtcac gtcgccgtcg ccgtcgtaat ccacagcggt catgcggatg
      361 gtgcgcaggc cttcgagcgt gtccgcgccc tcatggcatt cgccgcaatc cttgatgacg
      421 atctgcagcg cgtgggtgtc gtggcactcg gtgcaggagt tgaacgtgcc gacatgctca
      481 ttgaagccgg tatattcctt gcccgcgaac tcgtaagcgc ccttcacttc cgtgccgaac
      541 agcgtcgcgc cggcggcgaa gtagtggata ttgaggaagc gcagaccttc ggatacctcg
      601 tcacccggca gatcgccgat gaggcgattc acgctgacgg tcgactcgcg gccctgatgg
      661 cagttcatgc acatgttggc ggccgaatcg cgctcgccga agctgacggt cgccccactg
      721 gggaagcgca ccgcgggaac gtcgtagagc gtgaagttct gcacgtcgtt gtggcaggtg
      781 ccgcaggtga ggccgttgcc ggccggctgg ctggtcacca cgccctcggc gatgaactcc
      841 ggctggccgg tggccgagtg gcacttggcg catgagccgg ggacgatgcc gccctcagca
      901 tcccagtggc ggaacgcctc ctcggagccg gcaaagtggc cgtgatcgat gcgattggcg
      961 gccgacagat cgaccggcgc atcgacggcc tcgttcaggt ccgtgatgga gtcatagagc
     1021 aactggatga tgtatttgcc gccgtgggca taggcgccgg ggtccttctt ggagaccacg
     1081 tagttgtagg cagacttggc cagtcggggc gtccaggcgt tgaacgcgtt gggatagact
     1141 gcctcggcct cgctcaacgt tccgtccata tcggtgtcga taaagaagta aggataggcc
     1201 gtatcgctga aaccgatcat cgagccggtc gtctccacgg agtacatctg caaggctcca
     1261 tagagtttct cgcgcaagcc ctcgatctcg taatagatgc cttccttcat gtcgccgtcg
     1321 ccgtcgtagt cgacgagcga gccgttcatg cggatgttga ccaggtcctc agtcgtcttg
     1381 acatcggtat ggcacgcctg gcactccgag accttgatct cgagcgtgtg catgtcatgg
     1441 cattcgatgc aagtgttgta ggtgggcacg tggtcgaacc gggcatcgta gggctgcccc
     1501 atgtattcat agccaccctt ggccagtgtc ccgaactggg tcgcggccgc ggcgtagtaa
     1561 tgaatatttg tgaagcccaa atcctcactg gttgcatcaa ccttttcggt cagaccggca
     1621 tcagcgatgc gctggtcaac cgagatggtg gacgcgcgac cctgatgaca ctccatgcaa
     1681 cgcgcttcgt cgcccagatt ggtgatctcg atgcccgagg ggaacaccac gctggtcttg
     1741 gtgagcgtta cctggttgtg gcaggccgtg cactcgatgg tcgtgccgat gggtgccggg
     1801 gcgtctacga cgccgggcgt gctcccgtcg gcgccgaggt ggtcgatata gccgggcgtg
     1861 ctgtggcact tggcgcaatc ggtcggaacc tcggccgggt cttcctcgtt ccagtgattg
     1921 aaaggctcgc cctccgtatc gttatgcgga gagttggccc actcgtcgta gaacggaaca
     1981 gccaccgagg gagccgattc taccggcacc tctacgatgc gggtgacttc gacttcggtc
     2041 actgcccccg ttccaccgct ttcctccgag accgcggccg tcgcctccgc cacggctccc
     2101 gccgtgggtt ggctcgcccc ggtcccctgg cctcctccgc tggtttggcc gccctggttg
     2161 ctgcaagcaa ccagcaaccc caccaacaac aggagagcca ccaacaatag atagatgagc
     2221 ggacgcttgt tgggtctcat ttaaaaaaac ctccttaaag ttttttacca aattgtgatg
     2281 ccggccgggc gtcgctctat gtccggtggg caccttgtat cgaactggct cccggcccct
     2341 acgggtaagg ggttgccagt gggacaaaca cacctcttgt cgctgccggc tccatgatga
     2401 tggccgtgtc ttcgaatgtg aacatccaga tgaccaacgc cagggccagg acagtgacga
     2461 cgctcgcgac ggcgaagtag atgcgccgcc gtcgggccaa aattttgggc gggacgacct
     2521 tccagggcgc gccgcctgcc tccagacgct ccagttcgag cgcgtgttct tcctccatga
     2581 tcttgtgcga cagcgtgccg gtgaacatgc tcacgttgcg gtgcttcacc agcacgttat
     2641 agagatgcca gatgacgatc gacacggccg ccagcaccgc ctcccagccg tgggcctcga
     2701 gggccaccgg aatgagatcg ccggggatga tttgcgtcgc cgcaagaggg ttccacagga
     2761 tgaagccggt gaaaatcatc acccccgtgc cccagacgac ggcccagtac tcgaacttct
     2821 cgccgaagtt gaacttgtgc atcctgggcg gctcgtcgat gaagcccagg ttataggcca
     2881 cggtgtcgcg caggtcgtag ccgtccttga gttcgggcag catgcgcatg tcctcgcgct
     2941 tgacgaacag acgataagcg gatgtgagca ggtggtagac cgtccccagg atgaggacga
     3001 cggccgccca gcggtggatc atgcgcgcga cctcaatgcc gccgatgatc tgaatcagcc
     3061 cttgcgacac gggcgagaag gggaacttct gcggcaaccc ggtgaggcag aggatggtga
     3121 aactgatcag cagggtcatg tgctcgatgc gcgccatgag ccggaagcgc ggatattcgg
     3181 ggccgtgcgg ggtcatgacc gctgcgcctg ggtcatgggc cacttcgtgg gccacttcgt
     3241 gagccacttc gtgggccact tcgtcggccg gcagttcgcc aggtgtcaca ggcacgggcg
     3301 gcgtctccac cgaggcggca agcacttccg gagcaggggg cattaccggt gcttccggtg
     3361 ccgccggtgc ttccggcgcc gcgccctcag ggacctttgt ctccggcgcg atcgcttcgg
     3421 gttcgataga agaatcgtcc attcgtttca acctttcgtt atcgcccacg catctgacga
     3481 tagacatctg tgcccaccag gaaagtcagg aagagcaccg tccccggaat gaccatggca
     3541 tagaagatgg ccaccaggag catgagcggg ttctcccgca acgacggccg atgatggccc
     3601 gtccatgagt tcgagaagtt ggccgtcgcg ccgggatggc attgctggca tgtctccagc
     3661 aagttctcct tgatgcccga atcgggatcg ttcggccgcc ggatgtcgtg gacgccatgg
     3721 cagtcgtagc aaacggcctt gttcgtagcc acgttgggat cgctggattg gaagatcgtg
     3781 accgttgtgc cgtggaaatc gtccacgtag gtatcaaata catccgtcga gatgtcgtat
     3841 tgcgacatta tctcttcgtt ggcgtggcag gaagcgcaca gctccggcga acggttgcgg
     3901 aagaggggcg ttgtgggatc gcccacgtca tggacgccat ggcactcgat gcaggtgggc
     3961 acgttcgggt cctcgtcgcc caacagcgcc gcaccgtgga cgctttcggc gtattcatcg
     4021 aagatggcgc tatgacattg gcggcaggtc tcggagatgc gcgagcgtgg ctcgctgggt
     4081 ggtggagtgt aatgagagct gtggcaatcg acgcaggtgg cggcgtcctg gttgccgtcg
     4141 gccaatgcct gggcatggga gctggacatt tgcccttcgt gctgcggttc atggcagcgg
     4201 gcgcataatt cggattgctg tatggtgtag tcgcgagccg tttcggcttg caggggtgga
     4261 tgggggtagt cgtagtcctg atgacagtcg gtgcagttca gcgcttgcca gctgtttccg
     4321 atgccgtgga ctgaggcgtg gaggccctcg ccgtcgatgc tgatcgaaat ttcgtcccca
     4381 ttggggaatg tcatcgtctt gttgggcggg ccgtgacagc ccatacagaa gtcgttgttg
     4441 atctgctttt gcgcgaacag gcccgcctcg atatgccggc tcagactgcc gggatcggtc
     4501 agggcaatgc ccgcgccggt gtggcagtcg acgcaaacgg tcgtcgatgt gggcagccgc
     4561 cacgccttgg ccgtctgcac gtgatggtcg ccatggcaat cggtgcaaac gaccggcagg
     4621 cccgactccg acgcggccac gtcataaccg cccgaccatt ccggccccgg atgggcggtc
     4681 agatggggat cgtggcagtt gacgcagctc tgtgactgga ggatggtgta ctcgcgcagg
     4741 gtgccggctt cgggtgaggg gtggggaaac tggtagcgag cgggcgcgtg gcagccgctg
     4801 cagccgatga acgcgccgga gtccgcgccg tggatcgagg cgtcgaagct gcgcaggtca
     4861 acctcgacgg gcaacgattc ccccgaagga aactccacca ccgcgtccgt gtcgctatgg
     4921 catgaatgac aggcaaagtc ggtcgtcggg tgaatggccg gctgcccggt ttgggccggt
     4981 tgatcgagcg acggcagcaa aatcgtgatc ggcccgacag gaggagcgcc ggatggcgcg
     5041 acgggctgct cggcccgcgc cgattccatg ttggcgaaga acgacaacgc caccaggaaa
     5101 gcgatcaaga ctggagccac gacgcgtggc ccgactttac ttacttgacg caaggcgcct
     5161 acctctcaat ccgtaaaacg atcatggttt gctcttcttg tctagacctg acaggtgggc
     5221 gacaaagcct cttgctttgc cgagaccatt tattgctcac ctgtcaggtc ttcaggctcc
     5281 ggctcgtgat acccctgggc cgcgcccagc aacgcgctat ccggcaggtt tggcccgatg
     5341 cccagctcgc cgttatggca ttgacgacag gcataatcca gcccgatctg cggcaggatc
     5401 gtgccatcgg ccgcgaattg atccatttgg gtcggattga tgaccacctg atgggtcatc
     5461 atgtcggcca tgaatgactc cggccggccg atggcgttct gaatcaactg cggcatgtgg
     5521 caatcaacac aggccacgcg aatccgcagg tgaaccttct ctacctgcgc ctgctggaaa
     5581 tgacagccgt cacaggtggc ctcaaggaac ggctcatgat ccgcgcgcgg cgatacgacg
     5641 gccgtgtgcg ggtcgtggca atcgacgcaa tcgatcaggc ggtgcttgcc ggggaacaga
     5701 tcgttgtact cgtgaccggt gtgctggatg aacccgtcgg ccgtcaccag ctcgcccgac
     5761 atatgacaag cgcggcattc ctgattgtcg cgggagacct tcagcgcgaa ggaacggggg
     5821 ttgttgacgt gcaggctgcc cggtccatgg caggcctcac attgcacgcc cggctcgacg
     5881 aacccgccga ccatgttggg catgccgggc atgctctcgc ggccgctata gccggtggtg
     5941 tggcacgcgc cacaatcgaa cggtttctct tcgcccgcgt ggtaggccac atactcattg
     6001 ccggcctcca gctggttgtt gtcgaggtta aattgcgccg cctcgccggt gacgaggctg
     6061 ccgtcggtct tcacaaactg cgccttccaa ttgaaaccgc ccacgacata ggcgatatcg
     6121 gaccaggcca ggccctccgg cggcgtgcgc cgagcagtcg aggggaggaa ggcgggtgct
     6181 tcaccgttca cgacgggtgt gagggcgcgc gcatgaccgc tttgggcgaa ggtgttgaag
     6241 gtgtcctgat ggcagttggc acaggccgcg gagccgatga agcccgcggg ggtgaaactg
     6301 accccatcct gtccggccgg gccgcgcgca ccggcgagac cttgcgggcc ggtggggccg
     6361 ggcggccctt gcaacccctg ggagccttgg gggccggcgg ggccggggcg ccccgaacag
     6421 gcggcgacag cgagcaccag ggtcgccaat aacagccata tcgctgtgcg gcctacgttc
     6481 atgatagcct cccgtcgttt cacgtaaata ccagggtgga gctaccaccc gacagacgta
     6541 cagtatatct gatccccgat cacacctact actgctgatt gtcataaatt tcatatgact
     6601 tttgtatata tgcgcataga tttgctatga atcggcgacg gacggcggct aaattcggaa
     6661 aaaacgggcc tttcaggtat aatttaggtc attattcgtc ctctggattg cccatcgccg
     6721 atcacgatcg aacatggaaa aatcggccgc catgaatcat gccactgctt gtttcaatat
     6781 catcgtggct cgattatgtg gcgcaagggg catgaagtga accggagccg gcggaggttt
     6841 tagttggaaa caggaacaat caacagcatt gacattaacc aggagatgcg cagctcctat
     6901 ctcgattacg ccatgagcgt gatcgtctcg cgggcgttgc ccgatgcccg cgacgggttg
     6961 aagccggtgc accgccgcat cctctatgcc atgtatgaca tgggcttgcg gccgaatacc
     7021 ccccaccgca agagcgcccg tatcgtcggc gaggtgctgg gcaaatacca ccctcacggt
     7081 gacagctccg tctatgacgc gatggtgcgt atggcccagg agttctccat gcgctacatg
     7141 ctggtcgacg gccagggcaa tttcggcagc gtcgacggcg acagcccggc agctatgcgt
     7201 tataccgagg cccgtctggc atccatcgcc atggaactgc tggaagatct cgacaaggac
     7261 accgtcgatt ttacgaccaa tttcgacgac tcactggaag agccgagcct gttgccggcg
     7321 cggctgccca atctgctgct caacggcgcg tcgggcatcg ccgtcggcat ggccaccaac
     7381 atcccgcccc acaacctggg tgagttgtgc gacgccatca cctacctgat cgaccgtcag
     7441 gatgatatcg acgatgtgac tctggacgag ctgatggagt tcgtcaaagg ccccgacttt
     7501 cccaccggtg gcgtcatcat cggcaacgag ggcattcgca acgcctacgc caccggccgc
     7561 ggccgtgtgg tcatgcgcgg cgtggccaat atcgagccaa acccgcgcca tcccgaccgg
     7621 atgcagatca acatcaccga actgccctac caggtcaaca aggccatgct catcgagcgt
     7681 atggccgaac tggtgaccaa gggcgtcatc gacgacatct ccgacctgcg cgattcgtcg
     7741 gaccgcaacg gcatctcgat catcgttgaa ctgaagcgcc acacccagcc gcgcaaggtg
     7801 ctgaaccagc tcttcaaata cacggccttg caatcgacct tcggcgtcca gctattggcg
     7861 ctggtggaca agcagccgcg cctgctctcg ctgaagcgag cgctgcaaat ccacatcgat
     7921 caccgggtgg atgtcatcac ccggcgcacg cgcttcgaac tggacaaggc cgagcgccgc
     7981 cagcatatcc tggccggcct gctcatcgcc atcggcaacc tcgatgaggt catccagacc
     8041 attcgccagg cggccgacgc cgacgacgcc cgcagccaac tgatgacgcg cttcggcctg
     8101 accgagattc aggccacggc catcctcgat atgcagttgc gccggctggc tgcgctggag
     8161 cggcagaaga tcgaggacga gtacgccgag atcaccgccc atatcgccta cctgcaaagc
     8221 ctgttgcagg atcacggcaa gattctccag ctgatcaagg aagatttggc caagctgaag
     8281 gagaagtatg gcgacccgcg gcggacgcag atcctgcccg gcatggatga agagttcaac
     8341 atcgaagacc tgatcaagga tgaggatgtc ttcatctcca tcacacgccg cggctacatc
     8401 aagcgcaccc ccgtctcgat ctatcgtcgc cagtcgcgcg gcggtaaggg gctgatcggc
     8461 atgactaccc gcgccgagga cgaactggag catttcttcg tcgccggcac gctcaacacc
     8521 atgctgttct tctctgatct ggggcgggtt tacgccacga aggcgtatga gattcccgaa
     8581 ctcgaccgga cggccaaggg cgtatcgctc atgaacatcc tgcccctgtc gcaggaagag
     8641 aagatcaccg ccgtgctgcc ggtccacgac ttcgaggcgg gcaagtatct gatcatgatc
     8701 acccgcaagg ggcgcatcaa gcgcgtagag ctggggatgt tcgccaatgt gcggccgagc
     8761 ggcctcatcg ctatcaacct ggagacaggt gacagcctgc gctgggtgaa gatgacgcag
     8821 gggaacgaag acatcatcct cgtcagccag caggcgaagg gtattcgctt taatgaggaa
     8881 aacgtgcggc cgatgggccg gacggcggtg ggcgtcaacg ccatgcgcct cgacacctgg
     8941 gatcagctgg ccggggctga tgtcgcctct tccgacgacg atctgctcat catcaccgag
     9001 aagggctacg gcaaacgcac gccgctcgaa gaatatcgcc agcaagggcg ctatggtcag
     9061 ggcgtgcgag ccatgaatct cagccccgag cgcaccgggg ccatcgtcgc cgcgcgggtc
     9121 gtggggccaa aggacgaagt gacgctcatc tccagcggcg gtatcctgct gcgcatgtcg
     9181 gccgcccaca tctcgcagca aggacgcgcc tcgcagggcg tgcgggtgat ggatgtgcgc
     9241 gacggggata cggtggcctc ggtggctgtc atccgcgaga cacgccaggc ggtgactgac
     9301 gctgagatga acggggacga tgggcaagtg acgaatgacg aacgacgagt gacgaatgag
     9361 gagcgcagtt cgtagctcat aattcgtggc tggaaaagca aaaggggccg gttgattgac
     9421 cggccccttt ttgttgggga gagtcaggaa cgccgaaacc tgactctcgt taaaaaggag
     9481 gagaagaaga agagattgtt ttacccttca ttgactattc tatgacgaaa agtcaatccc
     9541 cgctgtacgt acgttctacg cgctagctac gggaaaccta cgcttgtgaa aaaaatcatt
     9601 atcgtgacct gaaaaaggcc aaggtagtgg cgaaggcaat accaccaggc agaactaact
     9661 tcatatgctg ctgtcacgtc ttcttgtggg ttgcgagccc cgaggagggt atggcaaacc
     9721 gggattaggc caaactgttt gccgaacaaa tagcgtggtg ttaattttgt tcgactggcg
     9781 aggatataat gaaaactcaa ggaacgagat ttgatgtata atgacaacaa acttatcaaa
     9841 tctaataatt attgacacaa gaaacattgg gcaatattga gggtacatat ggaggatttg
     9901 ttacaatggg ctgattatca aacagagcta gggggtatgt ttcaaggaaa atttgaggag
     9961 tggattcaac ggcccgaatt tgaattactt aatggtaaga tccaactcat ttttacatcc
    10021 cctccctttg ctttaaacaa caaaaagcgt tacgacaatc atcaaggtga agcgtataag
    10081 cattggcttg ccagttatgc acctatattt cgcaggcttc ttacagacag tggatctctt
    10141 gtaattgaga tgggaaatgc ttgggagccc agactaccag tcatgtcaac acttgcattg
    10201 gaggccttgc ttgatttgaa acgcgccggc gatttttatc tttgcgagca gtttgtagtt
    10261 cataatcctg ccagactccc ctctccagct cagtgggtta ctatcaatcg aaatcgagtc
    10321 acagacagtt ttacccatat atggtggctc tcgaatactc cttacccaaa agccgataac
    10381 cgttctgtat tgaggcccta cagtaaagca atgcttcgtc tactcgagcg cggaacatat
    10441 aacgatggaa agcggccgtc ggagcatgta gttggggccg aatcattctt aaaaaataat
    10501 gggggctcaa taccaaagaa tgtacttgag ttttccaata cttcctcaag tgatccttat
    10561 cagaagtact gtaaggaaaa cgggatcctc gtgcatcccg cccgcatgaa tattggcatc
    10621 ccaaattttt ttataaaatt tctaacagat tcaaatgata tagttcttga tccctttgct
    10681 ggcagtaaca caactggttc atcggcagag gcgctcggcc gttcatggct tgcatttgag
    10741 ccagtagccg attacgtagc tggttctcgt ggaaggttct caggattgga ttaacaagtg
    10801 aagcaaaact tcgagctacg tacctggcaa aaagaggctt tttcaatctg ggaaaggcag
    10861 atgcgtggtg ttgttgcagc agtaaccgga gcaggcaaga ctagcttcgc tctgttgtgc
    10921 atagagaaat tttttagaac tattcccaac ggagtagttc tcgtcgtggt gccaacccaa
    10981 gccttgctag atcagtggta tctcagctta gtagatgatg caggtgttca atcttcagat
    11041 attggtgttg ttgcttcagg acgtaaggat tggcgaggaa gaaagtatgt catctctgta
    11101 attaatagcg cacgggctct tgctcacaag attagcgaac aagcacccac aatgctcgtc
    11161 gtagatgagt gccatcgagc gggtagtgaa aagaatgctc aaattttcac aggcagtttt
    11221 tccgcaacac ttggcctttc ggccactcca gagcgtcaat atgatgatgg gtttaagcgg
    11281 ttaatacaac ctaatctcgg agatataata tatagatacg gatatgagag agcctacgct
    11341 gatggtgtta ttgcgccgtt taagctaaca aatattcggt tttctttaac acaacaggag
    11401 caagaagaat atgacatgct atcccgcaga atagcagttt tgcttcaaaa cactaatgaa
    11461 gatagtggag agcgaatcaa gttgatgctg ctcaagcgtt ctaggataag ttggaatagt
    11521 ccattaagag tccctctagc tgctagaata gctttggaac atcaaaggga tagagtaatc
    11581 gtgtttcatg agtctgtgaa gcaggcagac gaaatttacc gtattcttag ggccagaaat
    11641 ctacgaacta ctatctatca tactggaatg tcacctgagc accgtcgcga aaatctaagg
    11701 ttatacagaa acggatatta tacgagcctt atttgttgtc gtgcacttga tgaaggtcta
    11761 aatgtaccgc tcacctcagt aggaattatc gcaagtggta catccagtat gcgccaaaga
    11821 attcagaggt tgggtagggt acttcgtcag attcctggaa aggaaagtgc gataatatat
    11881 acgctatatg ctacagacag tgaggctaat aggttaatgc atgaagcaga caaaatggtt
    11941 gaaataacct ctgtaaaatg gttaggggtg aatttggagt tcagtggcta aaatcattca
    12001 tgaaggaaat gtgtatcagg aagtctcgct aacaatgttc catgagcgag aagttcaaga
    12061 ccagcttgcc agccgagcaa atctcgtttt ccctgggtat atttatgcag aattcatgcc
    12121 tcttgtgacc acgggtactg attcagcgaa accagatttt attttggttg aaaacgaata
    12181 tcgcagctgg gttgttggtg aagttgaaat ggttagacat gacctcacca atcatgttct
    12241 cagacaagtt caagtatttc tcaatggtga atatggagaa aaacatgtgc accatgccct
    12301 ccatcaatgt tcgagcctca atgagaaacg tcttcgtctt ctaattgaat caatttctcc
    12361 atctgtttta gtagtagctg acagacctga tgtagcatgg aaaaccgtca ttaatgggca
    12421 aggcgcaaaa atggcagttt tcgaagtctt ccgaagacat aactcagcag atctcatatt
    12481 tcgagtaaat ggagatattc cagaagtaca tggggatttt attaccaccc taaaatttga
    12541 tcgttcttta agacgcttgc taaggatcaa tacaccctca ggtttggcag ctgagaacgg
    12601 aaacaaaatt gaaattgagt ataacggtat gataactgaa tgggtggtaa tgatcgcaga
    12661 agataaggca tggctcaacc cagttggtca tccagatatg cctgacgatg caacctttat
    12721 gcttggaaca acatcagagg gatacttgag aatggaggtg gcgaaaaatg cagctcacaa
    12781 tcggtcttga agcaataaat tcttataaac gattatccta ttccacatgg catgcattag
    12841 cagaatttgt cgataattcg acccagtctt acttcgacaa tcggacgatt cttgatgagg
    12901 cattcagaaa agaggagtca aaattaaccg tttcaattct ttatgacaga gacagtaaaa
    12961 caatcactat taaagataac tcaattggaa tgtctgaaac agatttggag gaagccttaa
    13021 aaatcgcgtc ccggccacca cgttccaaca gtaggagtat ttacggtatg gggctgaaga
    13081 cagctgcatg ttggtttggt gatcgctgga cggtaacaac taagaaactc ggtgaaccaa
    13141 atgttatatc cgttactgta gatgttcctg caaccgcggc tggaaacggt aacctaactc
    13201 cgattcgaaa agaggcagat attgatcaac attatacaga gatcacaata tctcatttgc
    13261 atcggggaat aaacggacca gcagtaaaaa tcattaaaga atacttgtgc tctatatatc
    13321 gatgtgacct tcgcgagaat gttctagagt tattatttca aggaaagccc ttaaaatggg
    13381 aaggctttga ggatcggatc ctccgtgatg accaaggcca gttatataag agagatctgg
    13441 acttttacgt gcttggaaaa cgcgttacag gttgggtggc agtattagaa tcaggagggc
    13501 gtccgtatgg tggattctct gttctccaaa acaagcgggt tataaagggt tatccaaaag
    13561 ggtggcgtcc aagaaaaatc ttcggacagg aggagggatc taacgaccta ataaatcaac
    13621 gagtttgtgg cgagctattc cttgaggatt ttcaagtgag tcacacaaag gacgatattg
    13681 tttgggagag tgacgaagag gatatgatcg agagcgaact ctttattaga agtgaggact
    13741 atcgaagagt agcaactaaa caacgaaagg gtgatcagag agggccttca caactcgcgc
    13801 tcgataacgc agtcaatcaa gtatttaagg agctcagttc tgatgaaatg atcgacgtaa
    13861 tccgactaac tgagattccc ccagaatcta tccttaagca aacgagtcag atgactattc
    13921 aacatgcggc gaaggggaaa cctcgatttg atgtagagtt gggggatatg agagtcaggt
    13981 ttttcgttga ctcagatgca tcaccccgcg atacgtatta cgcacttgat tggaagccag
    14041 atggcacaat agagatcgct attaatcgta accatgatta tatggtccaa cttggaaatg
    14101 atgttgctga ctttataaga caatgtgtgt atgatggtat ttccgagttt aaggcaactc
    14161 agaagagagg ccgcgtagag ccaaatacaa ttcgctttat taaagataat ctcttaagag
    14221 tgccatatag aatccacgat gatacctatg agggggatga tgatttagaa tgatatggtc
    14281 ataatctgta cttttaaagt ctttgaccga atagtgattt gaagatcaaa cgtggcagtg
    14341 ataggcttca gaggtcttcc gatatctctg agatatggta gtgacaaggg aatcctcaac
    14401 cgcctggtgg ctcgcaatgg cggttcaagc cgcctacaaa atcgaatcgt ccgcaaaaaa
    14461 ctccttgaac gaccgctacc tatagccccg cagatttacc aagacgacca agggccatac
    14521 cgagagacgc ctccatcttc cctatcctaa agccatctct ctgatcaaca atcccgaccc
    14581 aatccgcccc aacaataaac acccatcctt cattcatcgt tcctccgccc tatccgagct
    14641 acaattcccc cctatgaccc gcctcacgcg ctaccacgcg ctggccttca tggttctcgc
    14701 cctgctcgtg gctgccgcct tgcgcctgcc tgacctgtcg gccattcccc ccggcgtcca
    14761 ctatgacgaa gccgcctacg gcctcaatgc cggcgacatc ggcctgcgcg gcgatcggcc
    14821 tatcttcatc cccgccttca ccggccgcga gccgctctac ctttacgtcg ccggaggcat
    14881 ggcccgcgtg ctgggcaaca cgatcttcgc cctgcgcctc acctcggcct tcttcggcct
    14941 gctgaccatc gccgccacct actggctcgg ccgcgagatg ctggccgacc ggcgtatcgc
    15001 tctgctggcc gcggcgctgc tggcgatcag cttctggcac gtgctgttca gccgcctcgg
    15061 cttcagggtc atcagccagc cgttgttgca ggcgctggct gtggcggcgc tctttcgcgg
    15121 gctgcgcggc ggccgttggc cgtggttgtg gttcgccggg ctgctcgtcg gcctcagcgc
    15181 ctacacctat ctggcggcgc ggctgtttcc cgtgctcctg gctttcgctc tgctgccgct
    15241 tctgctcgat gccggcactc ggcccgttcg cttgcgccag ctggccttcg tcgccctggt
    15301 cgcctttctg acggccctgc cgctgctggc ctacttttac ttcaaccccg acgctttctg
    15361 ggtgcgcatc ggccaggtcg cgccgggcga aagcggcctc agcctgaccg agagcctcgc
    15421 gcgctccttt gccatgttct tcctgcgcgg cgatccctat ctgcgcttca atctgccggg
    15481 gcggccgctg tttgattttg tcaccggcgg gttgctgctc gtcggctggc tgttcggcct
    15541 gctgcgctat cgccgcttcc cttatgattg gcagcgcgcg gccgtattgc tgcttttgct
    15601 ggctccgctg gtcatgatcc tgcctacggc gctggccacg aacgagatcg tgcccagcaa
    15661 tctgcgggcc atgggcctca tcccgttcat cttcttcctg ccacccattg gcctcatcgc
    15721 cttgctgcgc gacgtggagc gacgcttcgg ccgcccgccg ctcacccgtg ccgtatcctg
    15781 ggttgcccta ttcatcctgt tggccggtgg cacgtatacc caatatctct acttcaacca
    15841 ttgggcgacg caggatgagt tgctgctggt caacgacggc gacctgactg ccgccgccgg
    15901 atggctcgac gacacgctgg ccgccacgcc gctcgccgaa cggccggccg tctatgtcgc
    15961 cgccgtccat tatcgccatc cgacgatggc ctttctcagc gccaactacg acagcttcaa
    16021 ctggctgccc ggcagccacg cgctcgtctt tcccgcatcc ggcccggcgc tttatcttta
    16081 tccggccaac agccccgcgc cggcgtgggc gctgccgtat ctggaagcgg aaggcccgcc
    16141 ggaagtcgtc acggccgaga atggggttga actgttccgc gtctaccgtc tcagcgcgcc
    16201 gccgacgctg cccgccgcga ccgaaccggc caacttcggc gggctaatcg cgctggatgg
    16261 cgtcatcacc gaaccggcgg ccggtgggga gagcttgccg gtgacattgc gctggacggt
    16321 gacggccgca tcgcccgccg gtgtcgctta ttccacgttc gtccacctcg aagatcggaa
    16381 cggccaccgc tggagccaga tcgatcagga cggctacccc gccgggcaat ggcggccggg
    16441 cgacgtaatt ctggagcggc tcgacgtggc cgtgccgccg gggatgccgc cgggcgaaga
    16501 tggcgtctat cgactgcgca tcggccgctt cgatccggcc gatggcgagc gcctgccacg
    16561 ccttgctccc aacggcgcgt tcggcgggaa ttcgctgctc agcgacgacg tgacgattcg
    16621 tcacgccgcg ccgcccaatc cgctgccgga gccaccattc ccgatcaatg aggctgtgat
    16681 tgacgggttg cgttttctgg ggcatgaacc cattgcccgc gccgccgaga cgggcgagac
    16741 ggtgccgctg gcgctgtggt gggcggccga acaaccgctg ccgtccctgg ccatcaatct
    16801 gtcgctggta gggccggacg gcatagccca ccccttcggc tggacgcaac cggcctatgg
    16861 gacctatccc ttctctctgt gggaaacgcc ggcgttcgtc atcgatcgac aggatgtgcg
    16921 catccccgac gagttgccgt ccggcgaata tcgttggcgg ctggaactgg cgcagggtga
    16981 caagctggtc tatttggttg atctcggctc gcttagggtg acgcaggccg agcgcctgtt
    17041 tgaattgccg ccgttcgcca ccgcgatggg cgcaactttc ggcgaagaga tcgcgttgca
    17101 aggatatact ttagaaaccg ggtttcccgg aagaaccccg gtttccaact ccatcccgct
    17161 ggaactcgtc tggcaggcag tcgcccagcc gacgaacgac tacaccgtct ttgtccatgt
    17221 gttgaacgcc gacggcacgt gctgcgcgtg gcaatcggac gttatgcccc atggcggcac
    17281 ttatcccact tcgcgctggt ggccgggcga ggtggtggtc gattcatatg agatcgtcct
    17341 gcccgaagga gcatccatcg aggatcacgg gattgaggtg gggctgtacc tggcggagac
    17401 ggggcagcgg ttgggggtga tggtggatgg agaagcgaca ggggatgcag tcaggccagg
    17461 ctctggtcaa tagatacgag tacaataact cgcatccaga gcaaacaccc gagtatttta
    17521 ttactgaacc atgaaacgaa ctgcaattct atttgatatg gatggcttga tggtcgatac
    17581 tgagccgctg gcccgccgcg catgggagcg cacggtggct ctttatgggg cgagcattga
    17641 cgacgacctc tacaatcaga cgatcggccg ccgcacggtc gaaactaccc aactggtgct
    17701 ggatgccctg cgcctgccgg tggctcatgc cgacctgatg cggcgcaaaa cagacgaata
    17761 catgaatatc ctggccggcg gggttccggt catgcccggc ctgtggacgc tgctcgaccg
    17821 tatcgacgcg gcaggcctgc cctgggccgt ggccacgtcc acgccgcgac cggtggcgga
    17881 gatcgtcctg ggcatgctgg gcgtcacgga gcgatatcac gccctggctt gtggtgacga
    17941 agtgacgaac ggcaaacccg cgcccgacat ctttctgctg gccgcttcgc ggctgggcgc
    18001 cttgccggcc gcctgcctgg cgctggaaga ttcgcccgcg ggctgcacgg ccgccgccgc
    18061 agccgggatg cgtgtagccg ctgtgcccac ggacatgacg cgcagcgagg cgttcccgtg
    18121 cgcctatcgc tgctacccgt cgctggccga agtggcggcc gaccttgagc agctactggc
    18181 ggccgattaa ttcctgactt cgccgcattc catcagctgc atcgaggtaa tgccctcaat
    18241 ccagcattca gcgacgagat cgtcgccggt gatctgcatc agcacatgac tgccctgtcg
    18301 ggcgaggcgc tcgatgactg ggtcaaggcc gaagcgggcg cggtgctcat ccagccacgc
    18361 ctcagcctcg tgggtcgtcg gcaagatgca atcgcggcaa tcttccggcg gcggcatgcc
    18421 cgtcaccagg ctatgcagcc ccaggacgta ggggatcgag gcgtcgtaac agaagttcaa
    18481 ctggtcgtta acgacccggc agtaaatcca cgcgccggtg tcgggtatat ccacaccgat
    18541 aacgaccgtc gccattagcg ggttaacgtc gacgatgctc aacctgtagt ccccaccccg
    18601 aagcagttca tgtaccggcc gcagcgccgc gtagctgatg ccgtcgccca ggtccagcag
    18661 acgcaggtcg ccgtcgtagt cgcgcgcgat ggggatgact tgatgcacca gccgcgcggc
    18721 cgatgccgcc ggattggcca tcgtattgtt ggtgaccatg ctcgccagaa agacaatcgg
    18781 cagcggcagc aaaagcgacg cccagccgcg cgcgctaagc ccgccacgct gcccaatatg
    18841 gagcaagctc tgttccaggc gatagttttg taccaggccg aggcccgcca gtgccagggt
    18901 gatgaaaaat ccacccaaca acagcccgac gaccgtagct tccagcgtaa aggggtagat
    18961 atcgcggccg cgaaaacggc tgtcggttag ccagaccaat agcgtgcgcc cctgataggg
    19021 cagatagctg aggatgagca tgaacgccat gcccgccagc gaccacagga ggatggtgac
    19081 gggcgttcgg gcgatgcggc cggtcaacca gccaatcgcc gcccccatcg ctgtcgtcaa
    19141 tccgatcccg gccagcagca acggcaaata ggacgccatc ggcagccggg cgatttgcag
    19201 cacatccatt ccccatgctc ccagaccgac cgccagtccg atgagcaagc cgttgagcgt
    19261 gctaagccgg cgataggtac gcgcggcatt ttcgtcctct gccgagaggt ccagctcgat
    19321 ccggtctatc ggcctcggtt ccgccatagg tgaacgcctc caattagtgc aactataatg
    19381 ataccgaaaa tcgtgaacgt tgccgcgtca attccgccgg ctcgcggcgc atccgccgcg
    19441 accgtgggtg acgaaacagc cgcttccggt cgcagctgca ggtcggtcag attatcgatg
    19501 gtcaactccg tgccgtccca cagcccgtcc tcgcgagcaa tatgagccag aatggccagg
    19561 tattcctctt gagtcagaga gccgggataa aagtagggca tggctacgct ggcataacga
    19621 tagacagcat ccatcgtatc gaacttctgc agcgtgccgt caccgatgat cgcgggcacg
    19681 gccgtcggca gttgaaatcc ctgaccatag ggtcgctcgc cgtgacagcc gctgttccag
    19741 cagttttggt cttcatgggg atattgggcg cggaactcat cggttagccc ctgcccctgg
    19801 tcgccgtggc agggcatgca gtgcagccag aacagatacg cgccctcgtc ggcctgattg
    19861 gggctgacca ccgttggcgg cggagccagg cgatcgacgg tcggcatgac gccaggggcg
    19921 agcgtgggcg gcgggccggc atccggcgtt ggtgttgagg ccggaccgcc acgctcgatc
    19981 tgggccatcg tcatgccgta cagaaatagc caaagcgcgc ctgccataat cagagccgcg
    20041 ccgaaagcag caaatcgtcg taacatgtca tgtaccaccg gcttcctgac cggcgtcttt
    20101 ttcctctaac gccggtttcc tgaccgacgc tacaggcatt acaagcaaga aggcgggcgt
    20161 cttgctgcgt cgcccgcctt ccctatcatt ttgtaatgag gcgattgtat cgccattccg
    20221 acgccacgcg agcgcctgct cgcaactgtt aagctttctt tcctcgcagc ttcggatcga
    20281 ggatgtcgcg caaagcgtcg ccgatcagat tccagcccaa cccgtagagc aacaaggcca
    20341 cgccgggaaa gacgatgatg aaccagtatt catcgagcgt caggatccac gagcgggcga
    20401 agctgacgat ctgtccccag tcggcatagc cttccggcag gccgatgccc aggaagctaa
    20461 gcgcggcgaa cgacagcacc accgcgccca ggtcgagcga gatgcgcacc atcgtcggga
    20521 acatggcgtt ggggatgacg tgtttcatca tcagatggct gtccttggcc ccgctggcgc
    20581 gggcagccag cacataatcg cgttgctttg tcgccagaat gtcgccccgg agctgtcggg
    20641 cataacccgt ccagccaaat atgaccaggg cgaccgtggc aggcaagacg ctacgcccaa
    20701 agacaggcgt caacaaggac gcgaggatca gcgcaccggt caacgaggga aagacgatga
    20761 atatctccac gatacgcatt agcccctcgt ccacccaacc tccatagaat ccggccaacg
    20821 cgccgatagt gaccccgatc agcgtcgagg ccagagtgac tacgaagctg gcgaaaaacg
    20881 ccgttcgggt gccccagatc gtgccgtacc agatatcata ttgaccgctc gtcgtgcccc
    20941 acaggtgaac ccactcttcc tgacgcggcc agatgttacg ataccaccag ggcacggtca
    21001 tgggcgcatt tttgttccat gggctgccgg gcggctgggg gtcggcgcga aaaccgtcgc
    21061 gcgggatctg catccgcggc caattttcct tgggcggtgg agccaacacg ggcgcgaaca
    21121 gggccaccag tgcgaagagg acaatcacga tgatgcccgt gaccgaaagc ggattggtgg
    21181 caatgccgcg caacagccgc gccgtttccg ggccgaccgc cttctccagg cgcgtctgtt
    21241 cgcgtagttc gccgccgtcg agtgataccg ggccgtgatc aacaggttcg gtggttgtag
    21301 ctgtcatggg cttgtctcct ccagaaatta gctgagccgc acgcggggat cgaggaccgc
    21361 atacaggata tcaacgatca gattggcgat cacgatcagc acgccggtga agagcgcgta
    21421 accgagcatc gacaacacgt cgaggctgac cgccgccgcc gctgcccacg agccgatgcc
    21481 gggatagtcg aacacggtct cggtgatgac cacgccgttg agcaatccgg ccagcgttag
    21541 gccggcgacg gtgataaccg gaatgagggc gttgcgccgg atgtgacgat tgaccaccag
    21601 cttctccggc aatcccttcg agcgtgccgt cgtgacgtaa tcctgccgca tctcctccag
    21661 catggccgag cgcgtcacct tcagcaacag cgcgacctga atgaccatca gcgtcaccac
    21721 gggcatgatc agatggcgca gcgcgtcgat gaagatatcg aaccgcaggt taagcagcga
    21781 gtcgaaagtc atcataccgg tgtacttggt gaagcccgga cgcagcactt cctggctaaa
    21841 cgcgatcgac agtcgtcccg gctggaacca gcccagcttg gcatagaata ccagtaacaa
    21901 taacagggcg aagacgaacg tggggaagga ccagcccagg atggcaaaga tgcgcagagt
    21961 ctgatccacg ggccggttat ggttgagcgc cgccttgatg cccagccaga tgccgaaccc
    22021 gatgaccggc accatgctcc acaaggccag ctccagcgtg gccgggaagc gacggaccag
    22081 cacgtcgatg acctcctcgc gtccggtgcg cgaatagccc aaatcgccgc gcagcaaccc
    22141 gcccatccgc ttgccggtcg agccgtcttc gatgccgacc agccagttcc agtattgcac
    22201 gtagaacggc ttgtccagac cgtagcgggt gatgatcgcc tcgacctgac gatcggtctt
    22261 gggaatatcg cgcacataga gggctgaacg ctccaccggg ccaagcgcct gcaggaagat
    22321 gaagatgagg agtgtgacgc ccaacagaat gatcggcaaa atcaggagtc ggcgaatgat
    22381 gtatgcggcc ataacctttt ttatctccct tcaaatcatt cgaagcaaat aacttcagat
    22441 attcgcctct tttgtaatca gcaaacgcca caaaacgata tctgtgaatt cgctgtagca
    22501 ggcggctcaa cgctccctgg ccttcgccta aagcgctgag gcgctcgctc caactctggt
    22561 gccggtcggg ttgcggnnnn nnnnnncgca acccgaccct gcatagacct gtcaggccgc
    22621 ggatgacgat ggcgtttgat cgccatcgtc acctacgcct gcaggtgggc tggaacaaca
    22681 ggtttactga ccctggagcg acatcgtgta gacgaacgat ccggggacga cccgagccag
    22741 ttcctcgctg tagccattca cccagcgctg ctcatagcga gtgccggtct gcatggccag
    22801 gatgacggtc gggatgtcgt tgtaccattc ctgctggatg gcgaagtaca tctgctcgcg
    22861 ctttgccgga tcgctctcgg ccgcggcagc gccggccaat tcctggtact tgtccttgat
    22921 ctcctggggc atagcctggc ggccgccgta cgtcccgaag gtgtagggct ggatccagtt
    22981 atgcgggtca tggatgtctt cctgccagcc gctggcggca atcggcagct gcgacacgcg
    23041 gaaggagcgc aggaaggtcg gccagggcag accgatggtc tcgacccggt acaactcgtt
    23101 gatgccaccc aactcggatt gcagcatttc gccgatcgtc tggcgggcgg tgttgccggt
    23161 gttgaaggcg gcctggaagc ggaagccggt cgccggcagc gcgccgtccc atgcagcctc
    23221 aaagtatgcg gcgcactcct cggggttgta gttgtacgtc gggccgtcgg ggttgtagcc
    23281 gagcatgccc gtcgggatcg ggccgttgtt gcggataccc tcgccgagct gaacctcggc
    23341 gatgtaggta tcatagtcga agcaggtggc catcgccttg cggacgttga catcgctgaa
    23401 gaattccggc gggataccat tgccgtccag tttgccggag ccgatgtatg gcgaatccgg
    23461 ggcgatgttc caggtcagga agatgtcggt ccgggagatg ccggggatgc cggtccagcg
    23521 gcgcagcggg gcatcggggc tttcggtcgg cgagcagccc tgctggtaga cgcaggcttc
    23581 acccacgagc gggtcgacct gcgggcggtc ggcggtgttg atcgccacca cagcggcgtc
    23641 gccggcttgc agcgcggcaa agcgggtgcc ccactcatca acaaggcggt agatgaccgt
    23701 cttgatcgcg ggcgggccga acgggccacc ctcgaacaac ggggtttccg cggtccgcca
    23761 gtagttctcg ttggccgtca ggacatactc ttcgccgggg gtccagtgat cgagcatgta
    23821 ggggccggtg ccgttggcca ccgcgccgag cggggtgttc tccgcgccgg gggcgtagtg
    23881 atcttgccag gtggcgcaat cgccgtccca gctgccctga gcgacagccc agtcttgatc
    23941 aatcacgctg ccccaggtgc cggcgagggt cgccagcagc ggcccccacg actgggccag
    24001 attgaaggtc acggtgccgg cggcgtcgtc ggcaacgacg gcggccttga ccaattcaca
    24061 ggtggccagc agttcttcgg gcgtggcccc ggccttcagg ccttcgggat cgccggcgaa
    24121 ggcgccttcc tggatcccct cggtgacgtc accggaggta tagcccagga tcggctccag
    24181 gaacagccac tgcgggccgt tcgggtcgct ctgcagcagg ccgcgctgga agctgtaggc
    24241 tacgtcgctg ggggtcagcg tggcgccgtc atggaaggtg acgccctcgc gaatgtggaa
    24301 ggtgtaggtc ttgccgtcgg ccgagatgcc gccgttttct attgatggaa cttcggtggc
    24361 cagagccggg acgaacttgg tcgggtcaag gccatcatag aagatcaggc tctcatagat
    24421 atgcatgatc gtggtgttgc tggccgtatc atagcccagc gccggatcga gcgtgtccat
    24481 gtcgccaaat gtctggctga cgtaggtcgt cggatcggga gcggcgatcg ccgcgggcgg
    24541 gaccggggtc gccgtgacga cgacttcctg gacctcggtg acgaccgaag tgacgaccac
    24601 ttcctgccct tcctcggtga tagtctcggt gacgacgcgc gtcacttcaa ccgtctgcgt
    24661 ttgcggctgg caggcgacta atccgagcgc caaaaccagc agcgcggcca tcaataggcc
    24721 aatgcgattc ttggtaaaca tgttttgtgt ctcctccatc atctaaatgg ataaactgca
    24781 tttgtcgaat gtgtgcgcca gctacgcacc agtcaagtta aacctgaaat cctctctgct
    24841 tctcctgctt ttgacatcac ctcctcaagg tgaattacga attacgaatt aggaatcagg
    24901 aataaagaac tgtttttcgc cactatcttc attcgtaatt cgtaattcgt aattcgtaat
    24961 tgatcacata aatttctccg cgtggtgaca ggccaccatg tggggcacgg cggccgtgcc
    25021 catattacga aactcggggt ccacttcacg gcatatctcg gtggcatagg cgcaccgggg
    25081 gtggaagcgg cagccggacg gcgggttgac cgggctgggc acatcccctt ccaggatgat
    25141 ccgctgtcgc cgggcttcct tgtccgggtc ggggacgggg atggcggaca gcagcgcctg
    25201 ggtataggga tgcagcggat ggtcataaac ctcatcgcgc gagcttagct ccacgatgcg
    25261 gccaagatac atcaccgcga cccgatcgct gatataacga accatggaca ggtcgtgggc
    25321 gatgaacaga taggtgagcc ccagttcgtg cttcaggtca tcgagcatgt tgacaacctg
    25381 ggcctgaatc gagacgtcga gggccgagat aggttcgtcg gcgacgatga agggcgggtt
    25441 tgttgccaga gcgcgagcta cgccgatgcg ctggcgttgg ccgccggaaa attcatgcgg
    25501 gtaacgattg acgaaataag ggttcaggcc cacaacgcgc agcaactctt gcacgcgttc
    25561 cttgcgggag gcggcgtctc cgatgccgtg gatagcgagc ggttcgccaa tgatgctgcc
    25621 gacagtcatg cgcggattga gcgaagcgta ggggtcctgg aagatcatct gcatgtggcg
    25681 gcgcgcccgg cgcagatcgg acttgcccat ctccgtcagt tcgcgatcgg cgaacttgac
    25741 cgaaccggcc gtgggcactt ccagctgcag gatggagcgg ccggccgttg atttgccgca
    25801 accgctttcg cccaccagac ccagtgtttc gcccttatag atatcgaagt cgatgccatc
    25861 gacggctcta acatgaccga ccgttcggcg caggacgccg cgcttgatgg ggaaatactt
    25921 cttaaggcca cggacctgaa taagggcctg gctggatgtt ggtgcaagtg aggaagcctg
    25981 cgactgaact tcggcgttgg atatattctc agtcatcacg ccacctcctt cagggtcgcc
    26041 agggcttctt tattgatatc gtccatttgc tcgttatcat gaatgcgcag cccagcgaac
    26101 acttcgtcgg cgcgccagca agcggagtaa tggtcgggag acaccggcat aagcggcggg
    26161 ttttctgtcc aacaatgctc gatagcgtaa gggcagcgtg gtgcgaacgg gcagcgggtc
    26221 ggctctttca acaagtccgg cggcagacct ttgatcggga tgagacgctc tttcttggtg
    26281 gcatcgactt taggcagcga ttccagcaga cctaccgtat aagggtgggc tgtttcctta
    26341 tagagcgcgc ggacgggcgc gttctcgacg atgaagccgg catacatgac gatgatccga
    26401 tcgaccatgc cggctataac gcccaggtca tgggtaatcc agatgatagc catgcccagt
    26461 tgctgcttga ggcgcagaac caggtctacg atttgggctt gaatggtgac gtcgagcgcg
    26521 gtcgtcggct cgtcggcgat gagaagagag gggttacacg agagggccat ggcgatgccg
    26581 atgcgttgtc gttgaccgcc ggagaactgg tggggatagt ccttcaggcg atcagccgcg
    26641 ttgcccacgc cgaccaacgt caacagttcg gcggcgcgtt catcggcttg ctcctgtttc
    26701 atgcccagat ggagcaccag cgcttcggtc atctgcatgc cgacggtcag caccgggttg
    26761 agcgaggtca tcgggtcctg gaaaatcatc gccatttccc gaccacgaat tcggcgcatc
    26821 tcttcaggcg agagcttgat tagatcgcga ccgttgaaca tgacctctcc cgcctcaatg
    26881 cgaccgggcg ggctggggat gatacccatg atcgatttca ttgacaccga tttaccggaa
    26941 ccgctctcgc cgacgatggc catcgattgg ccttcgtcca aatcgaacga gatcccgttc
    27001 accgcatgga caataccatc ttcagtataa aaacgggtcg taaggttttt gacgtcaagc
    27061 agtattgtca ttcgtccccc gtgtgcgatt gaaaaagtcc aggagtcata accgcttcct
    27121 tatggctgca aggttgaata tcgtgaacaa tcgacctctt cacaatatta gagcgcgata
    27181 aatataacgc aaaacattgc attgccaaga aattcggcca gacacatgaa aagagcaaat
    27241 tcgacgcaca catcaggaag ttggcccgat ggttgcgttt acagcggatg tagtatagcg
    27301 aacttgattt caggtgacaa gttggtggca aaagaggtgc gcggggg
//
LOCUS       DAOMQG010000010        26311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_803_length_26311_cov_11.417048, whole genome shotgun sequence.
ACCESSION   DAOMQG010000010 DAOMQG010000000
VERSION     DAOMQG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 26311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26311
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_803_length_26311_cov_11.417048"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLC06_01385"
     CDS             <1..294
                     /locus_tag="PLC06_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89126.1"
                     /translation="WLILVYFGDLGLMGTAVVMKLKAGQLLALALINPLQVFKIAAVF
                     DLRNNLEVLGPAGIYAFRTYGTTLWPLLVGLLLTWVIVPFALATQVFKKRGVI"
     gene            291..902
                     /locus_tag="PLC06_01390"
     CDS             291..902
                     /locus_tag="PLC06_01390"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HRO89127.1"
                     /translation="MKHKTRNTGLGGRLKSFRPLRIRFWLMALGLLALSACGGSETYD
                     EPPEIMLGVDVCHACNMIINEGNFASAYWTKDGEARMFDDLGEMLRYMHHNPEEIASA
                     WARDMHTAEWIPLEDAWIVLNAGIHTPMGTGIASVLHEDDAQALAFDQDGAMILKFDE
                     IMKQLASGHLEIKMGGGHGGGMGGESMDGQSQDSHDGMPMGDD"
     gene            1006..1425
                     /locus_tag="PLC06_01395"
     CDS             1006..1425
                     /locus_tag="PLC06_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRO89128.1"
                     /translation="MMRKFTVLAVLAMLVMSLALAACGGGGSAPSAPAATTAPVATEA
                     PKGDPVAGKTQFDSVCIACHGPGGVGVTGLGKPFTTSEFLLTVDDQQLLDFIKTGRPI
                     SDPANTTGVDMPPKGGNPALTDAQLLDIIAYVRTLHE"
     gene            1546..1788
                     /locus_tag="PLC06_01400"
     CDS             1546..1788
                     /locus_tag="PLC06_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRO89129.1"
                     /translation="MNQDILPTNQELGNMMITDVLERWPRTAEVFHNHAMACVGCAVA
                     PFYSISDAAIIHGLVPEQFADELLAAIRDSRGSIKN"
     gene            1792..2112
                     /locus_tag="PLC06_01405"
     CDS             1792..2112
                     /locus_tag="PLC06_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRO89130.1"
                     /translation="MNEQYSFFPNLHDLAPAVPEDSILSRTFYKDERLSAILFSFAAG
                     EELSEHTSAYPAILYFLSGRARLKLGEDEFMVEAGAWVRMTPKLPHTVIAETPVVMQL
                     LMLR"
     gene            2235..2666
                     /locus_tag="PLC06_01410"
     CDS             2235..2666
                     /locus_tag="PLC06_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89131.1"
                     /translation="MPPLTRWHIKSAFIYLAAAMLLGIGLAMGAVVQIPNWLSYLSPS
                     FFHLIMVGWVTQMIIGVIFWMFPIVTRAKPRGNEKFGWATYIFLNVGLIMRVLCEPLN
                     AMNPQGIWGWGLVVSALLQWVAAVLFVYNSWPRVKERYRGE"
     gene            2672..3079
                     /locus_tag="PLC06_01415"
     CDS             2672..3079
                     /locus_tag="PLC06_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89132.1"
                     /translation="MPRLSQWYIKLSFCYLLLGFTFGALLLAHKGEPLHPALWNWLPA
                     HIEWLLIGWVAQLTLGVAFWIMPRYWKEPRRGNTTGAYVAIVLLNAGIWLATLGPVLW
                     LSPFVLIAGRVFEVVAALAFAHAIFPRVVGREG"
     gene            complement(3148..3561)
                     /locus_tag="PLC06_01420"
     CDS             complement(3148..3561)
                     /locus_tag="PLC06_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89133.1"
                     /translation="MAEDIKELKDVERKLMATDGEATDKDEGACDSQSGWIPGLVLIA
                     IGAFFLINNFTNFHLTNWWALFILIPAFSSLGSFVHAYRDGGWRNSKASGSLMMSFAI
                     FFVAATFLFGWSWGTVWPVLLIIGGLGALLSGFHD"
     gene            complement(3660..4073)
                     /locus_tag="PLC06_01425"
     CDS             complement(3660..4073)
                     /locus_tag="PLC06_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRO89134.1"
                     /translation="MMAEQPVEKILEETRTIAVVGFSSNAAKAGHYVPAYLKRNGYTI
                     YAVNPNVSEGLDGDPAYASLDEVPKPIDLVLLFQRSENVLPFVDQAIEIGAKAVWMQL
                     GITNQASAEKAAAAGLDVVQDACMLVEHQSWIGGE"
     gene            4357..5961
                     /locus_tag="PLC06_01430"
     CDS             4357..5961
                     /locus_tag="PLC06_01430"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRO89135.1"
                     /translation="MQNFGAFVSHYGVDNHGIKNPGTVYWNLTTAMLYEHVTRKDEGK
                     IAHLGPLVVQTGGHTGRSPNDRFVVKEPESESKIWWGKVNRPFSQENFDYLYRKMLAY
                     VQNRDIYVFDGYVGADPTYRMPVRIITEYAWHNLFARNMFIREFDPEKLRNHVPQFTV
                     IDMPRLKAEPDLDGTNSQTFILVDFGRRLVLIGGTEYAGEIKKSIFTAMNYFMPQQGV
                     MSMHCSANFGKDRDDVALFFGLSGTGKTTLSSDPSRTLIGDDEHGWSDDGVFNYEGGC
                     YAKVIRLDPKGEPEIYETTRKFGTILENVILDNSTRRVDLNDGSLTENTRSSYPISHV
                     SNAVRDGMGGHPNNVFFLTADAFGVLPPIARLTNEQAMYHFLSGYTAKVAGTERGVTE
                     PQPNFSTCFGAPFMPLRPGIYAELLGDKIQKHGAKVWLVNTGWTGGPYGVGSRMKLAY
                     TRRMITAALNGELDDVPMVEEPFFGLSIPQAIQGVPSEVLNPRNTWSDKDAYDAQAQR
                     LVGMFKENFKQFEENVSNEIKAAGPR"
     gene            complement(6028..7521)
                     /locus_tag="PLC06_01435"
     CDS             complement(6028..7521)
                     /locus_tag="PLC06_01435"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRO89136.1"
                     /translation="MQKKLDELKDRLREVEDLNLVTALLNWDQSTYMPPGGAPARGRQ
                     MALVSRLAHEKFIDPAIGRLLDDLRGYEESLDPGHDDAALIRVARRGYEKAVRVPPQL
                     LSELCEHSARSYQTWTTARPADDFAAVEPMLEKTLDLSRRIADCFPGYDHVADPLVDF
                     ADEGMKAESVRALFADLRAQLVPLLREVLEQPEVDDSCLHQTFPEEGQRAFGEMVIRQ
                     LGYDFNRGRQDKTHHPFMTKFSLGDVRITTRFQEDFLANGLFSTIHEAGHAMYEQGID
                     MKYEGTPLAGGTSAGVHESQSRLWENVVGRSRVFWNHYYPQLQTYFPQQLGDVELETF
                     YKAINKVQQSLIRTEADQMTYDLHVMIRFDLELALLEGSLSVHDLPDAWRSRYQSDLG
                     VSAEQERNGVLQDVHWFAGTIGGVFQGYTLGNIMSALFYQQALLAHPEIPSQIGQGEF
                     KTLHDWLELNIYRHGSKYTANELIQRVTGGPLTIEPYIAYLRGKFLD"
     gene            complement(7577..8395)
                     /locus_tag="PLC06_01440"
     CDS             complement(7577..8395)
                     /locus_tag="PLC06_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRO89137.1"
                     /translation="MSGSIRIALGVYSPQLDNKRDLLVYLPPSYHHSERRYPVIYMQD
                     GQNLFDSDTSYAGEWGVDETMEHLGREEGLEAIIVGIPNAGIHRLDEYSPFRDARLGG
                     GRGDDYLRFIIDTIKPQIDGEFRTLPEPKYTGILGSSMGGLISLYAFFEYPTVFGYAG
                     VMSPSLWFASGAIFDYIDAASFHPGKIYLDAGTREYGESHGGGRLHRAAASRRYYASV
                     RRLKGMLVRKGYRPLRDILHVEEKWAEHSESSWARRLPPALRFLLSEALRQTHG"
     gene            8690..9229
                     /locus_tag="PLC06_01445"
     CDS             8690..9229
                     /locus_tag="PLC06_01445"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HRO89138.1"
                     /translation="MNSSNDDERRQEEESGSLDEHQRRMRFQWNDLIEDLIEDGRRRG
                     LFEDLPGKGKPLNLEQNIFEGNSTLANQLMRANDIKPIWLAQRIGVLERIGELRADMA
                     RAWERYDIAFSHVYGDSHRQALTIGWDEQCQRWQKTIEKLNKEIGTYNLKRPDGQPEL
                     IKLRLNDELERIDAPRYLL"
     gene            9518..10795
                     /locus_tag="PLC06_01450"
     CDS             9518..10795
                     /locus_tag="PLC06_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO89139.1"
                     /translation="MTTTLVDNTEYISPNERLDYFRKIDSANIPSVTRKLIEEPQHND
                     PSSWLEGPDRGNPPNNLEAIEDHENIIAIDETGNGSDEAEAESFVDVEGYLDEYDRQQ
                     AGDKYESLQKLTQQRRSALDDVPTADFLSRYMLETNRQRLLTAEEEVELAKQIELGNF
                     AAQRLNEEDIEAEELAELQRIIDQADAARTLLVRSNTRLVISIAKRYFGQGLDFLDLI
                     QEGNIGLLTAVNKFDYTRGTRFSTYATWWIRQGITRALSNYGRMIRIPAHQTGNVRKL
                     YRAIRDIEQNQGRQADVEELAQYVELPSSQVRWLLQITRPLLALEQPAGDDQDAELAD
                     FIEDRDAEQPADIVDTKLFGEQVGEILAELTPREAAVIRLRYGLQGTDAHTLKEVGEI
                     FKLSRERIRQVEKSALSKLRHSHSLGDYAYMMN"
     gene            complement(10829..12226)
                     /locus_tag="PLC06_01455"
     CDS             complement(10829..12226)
                     /locus_tag="PLC06_01455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRO89140.1"
                     /translation="MSPVDVAWLSMDEPTNLMMVNAVFMFDEPLDIDRVKKVLEYRWL
                     QYERFKQRVIRPAIPFFRPYWETDPNFNLDMHLQRIALPAPGDQAALQDMVSDLMSMP
                     MDFSRPPWKFYIIENYGEGCAVMARLHHALADGMALVAVLLSMTDFYADAPLVPTETM
                     NPLPDGGLISGLAREMTSTVDLFRRTADRALQGAVSAVQDTDKARQIGEQGAGLGLAA
                     TRLLARSADPKTRLKGKLGVMKRAAWSGAIPLADIKLIKSNLGGTVNDVLVTVVAGGL
                     RRYLVAKGDDVADLEFRAAIPVNLRGEEDGGKLGNKFGLVFLTLPIEIEEPLMRLTEV
                     RRRMEALKRSAEAPVTLGVLGMMGMGTDRFRELVVRILEPKATIVLTNVPGPPIPLYM
                     AGQKIKEIMFWVPQAGRLGVGVSILSYAGNVYLGIATDAGLVPDPDTVLEGMYAELER
                     MKVLAQAVSSSNGHQ"
     gene            12392..12464
                     /locus_tag="PLC06_01460"
     tRNA            12392..12464
                     /locus_tag="PLC06_01460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12425..12427,aa:Thr,seq:cgt)
     gene            12553..13215
                     /gene="udk"
                     /locus_tag="PLC06_01465"
     CDS             12553..13215
                     /gene="udk"
                     /locus_tag="PLC06_01465"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRO89141.1"
                     /translation="MSSPKYKPVVFGVAGGTASGKTTVARAILDAVGASQIAYLPHDA
                     YYHDRVDLSFEDRARLNYDHPDSLDTKLLVRHIKDLRMGSPVHVPVYDFTTDRRTEDT
                     ILVEPAPIILVDGILIFTKRKLRDLMDIKIFVDTDADVRFIRRLQRDMKERGRSLDSV
                     VQQYLETVRPMHLKFVEPSKRYADIIIPGGGQNKVAMEMVVSRLQMLLKVREEQAEEE
                     IA"
     gene            13215..14183
                     /locus_tag="PLC06_01470"
     CDS             13215..14183
                     /locus_tag="PLC06_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRO89142.1"
                     /translation="MINWSEQRVVVTGGSGFLGRHVVGALQRRGAGNVVVPLREDYDL
                     RDQGAILDLLNDARPTMVIHLAASVGGIGANLAHPAEFFYDNLMMGAQLLHESWRSGV
                     DKFVAIGTVCAYPKFTPIPFHEEDLWNGYPEETNAPYGLAKKMLLVQSEAYRQQYGYN
                     SIFLLPVNLYGPGDNFDPESSHVIPALIRKCLEARQRGDERIVAWGDGSPTREFLYVE
                     DAAEGIVMAGERYNQSDPVNLGSGFEISIKDLLQTIARHTGFTGQIEWDTTKPNGQPR
                     RALNTDRAFEYFGFRATTDFDEGLNKTVEWYQQTIADSSQTLVRPQ"
     gene            14206..15693
                     /locus_tag="PLC06_01475"
     CDS             14206..15693
                     /locus_tag="PLC06_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRO89143.1"
                     /translation="MSLAQRSITSVAWNVFANLFKIAILLVRSIWLARLLPVETFGVY
                     ALATSIVTFTGVIPMFGMASAFLHRSPETANEEQAAAVHFTLRLVLTSCWAGILILAA
                     FLFTSAELRLALIVLTLVFSGLYLTDTPKAILTRRVEHRRLAMLDLLTALVTSIVAVY
                     LAARGFGLTALLATDIVTLILAVIMLYLWQPVWRPRLLWLRATVRYYLTFGGRAMLGG
                     LLSDALDHLDDIWVGAFLGNQALGFYSRAYTFATYPRRLIAFPVNMVAGGTYAELKSD
                     RLRLSQAFFRTNALLVRSGFLIGGLLVLIAPEFVSIALGNKWLPMIPAFRLMTVFTLL
                     DPIRITVSQVFLAVGQPDRIIRVRTVQLIVLIAGLFAFGLSLGIEGVAIVMNLVLLAG
                     LVPLLHAAREHVDLSVWRLFGAPTIALIIGVAAALGITYWVCLSSTCPNHWITGFSKS
                     IVFSLAFVGTIGSLEGKEALELIGRVTQIWRGKPMPNPDQPSSPE"
     gene            15690..16613
                     /locus_tag="PLC06_01480"
     CDS             15690..16613
                     /locus_tag="PLC06_01480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89144.1"
                     /translation="MNITVGITSYNQSALLRVAIESVLSQTRQPAEIIIVDDASTDDS
                     RHLIDSYVNKYPDLIRPIFLNHNGGPNNARNQVIDAAGGEFLTFLDGDDRWLATKLEQ
                     ETKRLTMADQPDAVFSNFYFTDGAGNREFMWVSDSTPRPPEGHILPQVLALNLPRNTL
                     FRSEIVPTRLWREAGYFDSSFTIYGDWDMRVRLAAAVGRFGYLDEPLCEYRRHGQGLS
                     NKPIEMHLAAVDHIEQKYAGLISQLNETTRKSPIRGLDKFRAKLLRRAALDSLLLPST
                     PARKQAIRYYQQSLRFDRVLDFRLFWYLLKA"
     gene            16700..17578
                     /locus_tag="PLC06_01485"
     CDS             16700..17578
                     /locus_tag="PLC06_01485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89145.1"
                     /translation="MDAANVPIREGTWPKITIITPSLNQGRFLEAAIRSVLMQGYPNL
                     EYIIADGGSTDDSLKIIRHYAPLLSSWSSEPDGGPAEALNRAFSRATGDILAWLNADD
                     VYLPGALFAVAEALEQHNDAALVYGEGWYIDESGRLIEPCRFVRRTFTRQFLVNKDPI
                     LQPAAFWRRELWERTGPLDTSLRWVFDWEWFIRAHTHGRFQYIPRDFAYYRVQPNALT
                     RTGGLPRQIEHGRVTRRYGTWWHPNHLVQQTRRLDAVGQKLTHGWPRVPAAIFRTPFA
                     IPRYLAEWLLHGMYMR"
     gene            17611..18648
                     /locus_tag="PLC06_01490"
     CDS             17611..18648
                     /locus_tag="PLC06_01490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89146.1"
                     /translation="MNVAFLPVYPNPYQKLLGNALAAEGVTVDLLESLPDNQWLRDNI
                     GRVQILHYHWLSGLYMNRFLTPLQVRKFCARFRLARDFGYRIVWTAHNILPHRTFLAP
                     LDKAIRRLMMAEADVVIAHCEYGRRELLARFPRKGPVVVIPLGHYRDAYPATLDRRSA
                     RERLGLHPDQFAYLALGNIAAYKGLSDLVHAFKQVAGINDVLFIAGRNRDQDVISRLL
                     QAAQTDSRIHIQPRFIPDDEMQIYLFAADAMVAPFRRVLTSSSVMTGLSFDLPQIVPA
                     LGCLPELVTPDAGIIYDPQDENGLANALTDIKVRDHDQMRAAARRITDGLGWADIARQ
                     TADAYRSCLTS"
     gene            18645..19802
                     /locus_tag="PLC06_01495"
     CDS             18645..19802
                     /locus_tag="PLC06_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89147.1"
                     /translation="MTGSGQKVAILSSVHLAMDNRVFYREARTLAQAGYDVTLIAIHD
                     RNEEIDGVHVRALPRVPRSRRPLLWRRLAQMALETEAGIYHIHDPELLLITPYLRRKS
                     GRPTIYDIHEANADFIAVKDYLPGLIRLPLSVLFRQLEPILARGEDGLIFADDSIAAD
                     FESVRAIKTTLFNFPGDELIMRGSLCPPPDPDVSPVILYLGGMERNRGSALMVAAFAE
                     VLEKRPDARLLVVGHFMPATLEDEIRAAAIAHNITHALTITGRVPFQKIGQYLAEATI
                     GWVTWQPFAKNERNIPTKLFEYMAFGRPVISSDLPSTRPFVEDGVNGFRVKADDPHAH
                     AEAILRLIENPDNAQAMGLAGREMVQTRFNWGRMIPRLLEIYDKVLSSRKT"
     gene            complement(19769..20785)
                     /locus_tag="PLC06_01500"
     CDS             complement(19769..20785)
                     /locus_tag="PLC06_01500"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRO89148.1"
                     /translation="MISSPILIIGCARSGTTLLYNLLSEEPSLWSIGGESKAIIERYH
                     APVTKDWVSGELVAEDLTAESAAFIHRQFLEQAAPGSYWYRVNRLRRSLNGNGLYRAV
                     KRHGRQGSQGASFSSAVPGAGLGFFRRVVELRNRLMPPGAPVRLLEKTPENCLRLPFL
                     AALFPDARVIYLTRDGRANIHSLVEGWRQPHLFPGYRTPVAVTSAGQARGRWAFTLIP
                     GWRDLVSSPLEVISAHQWVASNQAVLDYTNRPGALPALTIRFEDLVAAPAPTLTRVSE
                     FLGLSPDLIPAVDGPLPEVNAVSAPDLDKWRREAEMIARIAPIIEPMMERLGYVLRDD
                     NTLS"
     gene            20874..22067
                     /locus_tag="PLC06_01505"
     CDS             20874..22067
                     /locus_tag="PLC06_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HRO89149.1"
                     /translation="MRILFLTGRLPYPPNRGDRLRVYHFLRVLSQQHQITLLSFIGDN
                     RETSFIGPLRHFCEDIQLVHRGELQSTMSTGLNVWRPMPFQSLYYRSSVMQGAVDRLL
                     DRGRFDAVYVHLFRMAQFVAGRTQPYRILDLTDAISSEVERSLPYRDPKWRLVYRLEL
                     PRIRRYERDIVRHFDETWLISNAEKQKLSADNYNDKIQVVPNGVDSARYRPSGLPVDS
                     PRLIFVGHMGVFHNVDAAEHLVRDILPRVRAIFPNASLDLVGAEPAPAVRHLASTPGV
                     RVLGHVADLNAALNDASIFVAPLRFAAGVQNKVLEAMAVGLPIVTTTYVNDGLEAEDG
                     RHLLVADEPDQFADAVITLLKNTQLRRSLGRAGREFVMNHYRWEDVLDRVNLIQNQIA
                     HQQRR"
     gene            22151..26053
                     /locus_tag="PLC06_01510"
     CDS             22151..26053
                     /locus_tag="PLC06_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO89150.1"
                     /translation="MNSRLSRWCDGFLEAGWLVAILAIPLFFNIHSERVFEPDKLTLL
                     RSIAVLMAAVWVVRFIDQQGWRGLAEQRFSNPNSIWHKPFVLPVILLLLVYLLSTLFS
                     INPRISWAGSYQRLQGTYTTLSYAAIFAIMAGTLRTREQVRRVINVAIVTSIPVALYG
                     ILQHFGHDPLPWGGDVQTRVAGQMGNAIFIAAYLIMVIPLTLARIIDAFSNILGDERL
                     AAADVVRASVYIFTLAIQLLTLYWSGSRGPLIGLAVGLFSFLLVLLVSLRDATKDQAT
                     SRVKEALPALGFLLPAILALLISPIISATFGPMASFGFFFGVVALSVVSIFLMVSIRR
                     GWRWLWLSWILLTVLMAGWLLLFNIPSEQTAGLRGVPVVGSLFNTLEEWRNLPAVGTY
                     GRMLDPTDTTGREKSGRVRVLIWEGVIDLITPHTPLEYPDGSSDPFNWLRPLLGYGPE
                     SMYTAYNRFYPPELATVEARNASPDRSHNETFDALVITGLAGLLAWQALYLSVVHFAF
                     RYLGVIRSRRDTWVLIGLWVTGALLATLATIIINEPVYLGVAVPIGVIAGVIIYLIYY
                     ALFGRDQSVVAAEPSIAAFLPFSADRLLMNGLVAAVLAHYVEIHFGIAISATRLYFFV
                     YIALMFALGYRLRQPEPSTEEMPAVAPVPAKSSKRNRRTTVVPTEPTIRAGWQRLIVP
                     ALLLILMLGLFGYGFITYALPPDKVITGPADLTAGEIFRQSLFQNSRKGFVDWPFVTS
                     MIILSWALGWLVFLSEMAKYGEIKLASAGSIAGRGRAAAGVLFALALAGAGARFIIPA
                     GSITTTLGLSLGLIAALACALAAVLLIMKRPSGRLAGGITAASLIIFAFPLLIAGAIL
                     PATMMILGGAIVLWLLWDGNWRHSLLPIAGVILVSLLTGFAYIYVHAINYRSMLFYRG
                     SEQPASSTMLRVFEASQTSELIAFFFVFVFIMLLLLAFALSWSGLTSGRLPRTNVSPL
                     PAFGTLALVFIAAFFIISQSNVRQVQADMIYKRARPYDDQAARSSQARPEVRRDLWDT
                     AIAIYSAAIDRIPSEDFYYLFLGRAYLERAALTEDPTEQTELLSSAETLLLQAQALNP
                     LNTDHTANLARLNTRWYAAVGDGSEKAKRLDLAESYYEDALRLSPQNSVIRNELARLV
                     LELRGDCDRALALYDESATIDPFYSQTQLARADAYILCSSGRTDAERDTLYRAAAGAL
                     EEALADAPSNIRGWVQLAEIYRQLGEFDQAATAVENARANNGKATFPPSEIDFMDAQI
                     AAGLGDLDKARTLAEQALKTATPNTAEQIEAFLAELGD"
     gene            26115..>26311
                     /locus_tag="PLC06_01515"
     CDS             26115..>26311
                     /locus_tag="PLC06_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HRO89151.1"
                     /translation="MRAFLAVFLIALTITLGSLPWVRRLAIAMGFVDIPSHRKAHRTP
                     TPLLGGAAIFVGAILGVLIIYG"
BASE COUNT         5593 a         7745 c         7156 g         5817 t
ORIGIN      
        1 tggctcattc tggtctattt cggcgatctg ggcttaatgg gcacggccgt ggtcatgaag
       61 ctgaaggctg ggcaactgct ggcgctggcc ctgatcaacc cactacaggt gttcaagatc
      121 gccgccgtct ttgatttgcg caataacctt gaggttttgg gaccggcggg catctacgct
      181 ttccggacat acggcacgac gttatggccc ttgctggtcg gcttattgtt gacctgggtc
      241 atcgttccct tcgccttggc cacccaggtg ttcaagaaaa ggggagtaat atgaagcaca
      301 aaacacgaaa tacgggattg ggagggcggt tgaagtcatt tcggccgctg aggatcaggt
      361 tctggctcat ggcgttgggt ctgttggcgc tgtccgcctg tggcgggagc gagacgtacg
      421 atgagccacc ggagatcatg ctgggtgtgg atgtatgcca cgcctgtaac atgatcatta
      481 acgagggtaa cttcgcctcg gcctactgga cgaaagacgg cgaagcccga atgttcgatg
      541 atctgggcga gatgttgcgc tatatgcacc acaacccgga ggagatcgcc tccgcctggg
      601 cgcgggatat gcatacggcc gaatggatac cgctcgagga cgcctggatc gtcctcaacg
      661 cgggcatcca cacgccgatg ggaacgggca tagcgtcggt gctccacgag gatgatgccc
      721 aggcgctggc tttcgaccag gacggcgcga tgatcctgaa gttcgatgag atcatgaaac
      781 agctggcttc gggccacctg gagatcaaga tgggcggcgg tcatggcggt ggtatgggtg
      841 gcgagagcat ggatggccag agccaggata gccatgacgg gatgccgatg ggcgacgatt
      901 agcggccgcc acttggccga agcgcgcctg ctacggtata gtttagggtt gcaatccgtt
      961 ggggttgctt tgaaaaacca ttttatttga ggagagattc aagagatgat gcgtaagttt
     1021 actgttttgg ccgtgctggc catgctggtg atgagtctgg ctctggcggc ctgcggcggt
     1081 ggcggttctg ccccatccgc cccggcggct acgacggctc ctgtggcgac cgaggccccc
     1141 aagggcgatc cggttgccgg caagacccaa ttcgattccg tgtgcatcgc ctgccatggt
     1201 ccgggcggcg ttggtgtcac cggactgggc aagcccttca cgaccagtga attcctgttg
     1261 acggtcgatg accagcagtt gctggatttc atcaagaccg gccggccgat cagcgacccg
     1321 gcgaatacca ccggcgtgga tatgcctccc aagggcggta acccggccct gacggacgcg
     1381 caactgctcg acatcattgc ttacgtccgt actctgcacg agtaggatca acgaaaacca
     1441 taggcaggtt ggcaaccggg agaattctta accagatcgc tcggttgcca acctgccaat
     1501 ttagtgacac tcgcttagat agtcgaaaat gtgccaaggt gtgtgatgaa tcaggacatc
     1561 ctgcccacaa atcaggaact gggcaacatg atgatcaccg atgtgctgga gcgttggccc
     1621 cgaacggccg aggtgttcca caatcatgcc atggcttgcg tcgggtgtgc cgtcgctcct
     1681 ttctattcca tcagtgatgc cgcgatcatc catgggttgg tgccggaaca atttgccgac
     1741 gaattgctgg ccgcgatccg ggatagtcgc ggttcgatca agaactaacc catgaacgag
     1801 caatatagtt tcttccccaa tctgcatgat ttggcgccgg ctgtgcccga ggactccatc
     1861 ctgagccgca cgttctataa ggacgaacgc ctaagcgcta tcctctttag ttttgccgcc
     1921 ggcgaagaac tttcggaaca tacctctgct tatcccgcaa ttctctattt tttgagcggc
     1981 cgcgctcgcc ttaagctggg cgaagacgag tttatggtcg aagccggcgc ctgggtgcgc
     2041 atgactccta aattacccca cacggtgatc gccgaaaccc cggtagtcat gcagttgctc
     2101 atgcttcgct agaagcttaa gccgcataga aagccctggg gattgctacc gcctcactcg
     2161 cgcgcagcaa taatcaggtt tcatatttgg ccggaattag gatcaggtcg tagaataccg
     2221 cctgaggaat ttcaatgccc cccctaactc gctggcacat caaatccgcc tttatctatc
     2281 tggccgcggc catgttgctg ggcatcggcc tggcgatggg cgccgtcgtc cagatcccca
     2341 actggttatc ctatctctcg ccatcatttt tccacctgat catggtcggc tgggtgaccc
     2401 aaatgatcat tggcgtcatc ttctggatgt tccccatcgt cacacgcgcc aagccgcgcg
     2461 gcaatgagaa gtttggttgg gccacatata tctttctgaa tgttgggctg atcatgcgcg
     2521 ttctgtgcga acccctcaat gccatgaatc ctcaagggat ttggggctgg gggctggtcg
     2581 tttcggcgct gttgcagtgg gtggcggccg tcttgtttgt ctataattcg tggccgcgag
     2641 tgaaagaaag gtaccgggga gagtaagcga aatgcccaga ctcagccaat ggtatatcaa
     2701 attatctttc tgctacctgc ttctaggctt caccttcggc gcgctgctcc tggcccacaa
     2761 gggggaaccg cttcaccccg ccttgtggaa ctggttgccg gcccatatcg aatggcttct
     2821 gatcgggtgg gtggctcagc taaccctggg tgtcgccttc tggatcatgc cccgctattg
     2881 gaaagagccg cgtcggggga acaccaccgg ggcgtatgtc gccattgtcc ttttaaatgc
     2941 aggaatatgg ctggccacac tcgggccggt gctctggcta tcacctttcg tgctcattgc
     3001 cgggagagtt ttcgaagtcg tcgcggcgct ggcttttgcc catgcgattt tcccgcgggt
     3061 cgtcggccgc gaggggtagg cggctcgtgg atgacagttg tcgggtgccg ggcaaacacc
     3121 gccagcaaca cgaccatgag ggttaagcta gtcgtggaaa ccggataaca aggctcccag
     3181 accgccaata atcaacagca ccggccaaac cgttccccag gaccaaccaa acagaaaagt
     3241 ggccgcaaca aaaaagatgg caaagctcat catcagcgat ccgcttgcct tgctgttccg
     3301 ccaaccgccg tctctataag cgtgcacgaa gctgcccagg ctgctaaagg cagggatgag
     3361 aataaatagc gcccaccagt tcgtcaggtg gaaattcgtg aagttgttga tcaggaaaaa
     3421 cgcgccgatt gcgatgagaa ccagcccggg gatccatccc gactgtgagt cacatgcccc
     3481 ttcgtcctta tccgtggctt ctccgtccgt cgccattaac ttgcgttcca cgtccttcaa
     3541 ttccttgata tcctcagcca tggtgctaac ctccatctct ctatacgtga gcagtatcgc
     3601 caggttgtgc gttcatcgaa ggaatctatt atgcgagcta ctccgccatc tgctccaggc
     3661 tattcgccac cgatccagct ttgatgctcc accagcatgc aggcatcctg aacgacatcc
     3721 agacccgcag cggcggcctt ctctgctgaa gcctgattgg taattcccaa ttgcatccag
     3781 accgccttcg cgccgatctc aattgcctgg tcgacgaacg ggagaacgtt ctcacttctc
     3841 tgaaagagga gcacgaggtc aatcggcttg ggaacttcat cgagagaagc ataggctgga
     3901 tcgccgtcca gcccttcaga aacgttcgga ttgacggcat agatagtgta gccgttccgt
     3961 ttcagataag caggcacata atgccccgcc ttggcggcgt ttgacgaaaa tccaactacg
     4021 gcgatggtgc gtgtttcttc aagtattttt tcaacaggtt gttcggccat catttgtctt
     4081 tctccttatc atcattggtg tagattctac acatctaatc gattaagctc gagtccaacg
     4141 agagccaaat cgcaatttat cctaacgcct gtatagcgag caaagaacgt tggtgcaacc
     4201 tatgacggat cgcggggttg taataatagt gacttatctc atactgaaaa tgtgacaaaa
     4261 gatcggcatc atcaattgtc aatccggttg cgttgacata cactaccata agccactcgc
     4321 acgcacccac ttttccatta ttacaggagt aacgctatgc aaaactttgg cgcttttgta
     4381 agtcactacg gcgtcgataa ccacggtatc aagaacccgg ggaccgttta ctggaacctg
     4441 acgaccgcga tgctatatga acatgtaacc cgcaaggacg aaggcaagat agcccatctc
     4501 ggcccactcg tcgtgcaaac cggtggacac accggtcgtt cccctaatga caggtttgtt
     4561 gtcaaggaac cggagagcga atcgaagatt tggtggggca aggtcaatcg ccccttctcc
     4621 caggaaaatt tcgattatct ctatcgcaaa atgctggcct atgtccagaa ccgcgatatc
     4681 tacgtattcg atgggtacgt cggcgccgat ccgacttacc ggatgccggt tcgcatcatc
     4741 accgaatatg cctggcacaa cctgttcgcg cgtaatatgt tcatccgcga gtttgacccg
     4801 gaaaaattgc gcaaccacgt gccgcagttt acggtcatcg acatgcccag gctcaaggcg
     4861 gagcctgatc tcgacggcac aaacagccag acattcatcc tcgtggactt cggtcgccgt
     4921 ctcgtattaa tcggtggcac agagtatgcc ggcgagatca agaagagcat cttcactgcg
     4981 atgaactatt tcatgccgca gcagggtgtc atgtcgatgc actgcagcgc gaatttcggc
     5041 aaggatcggg acgatgtcgc gctcttcttt ggcctcagcg gcacgggcaa gaccactctg
     5101 agcagcgatc caagtcggac gcttatcggc gatgatgaac atggctggag cgacgacgga
     5161 gtgttcaact acgagggtgg ctgctatgcc aaggtgatcc ggctcgatcc caagggggag
     5221 cctgaaatct atgagaccac gcgtaagttt gggaccatcc ttgagaacgt gatcctggac
     5281 aatagcacgc gacgcgtcga tctgaatgat ggttcactga ccgagaatac ccgctcgagc
     5341 tatccgatct cccacgtctc caatgccgtg cgtgacggta tgggtggaca cccgaacaat
     5401 gtcttcttcc tgacggccga cgcgttcggt gttttgcctc ccatcgcccg cctgacgaat
     5461 gagcaagcca tgtatcattt cctcagcggc tacaccgcca aggttgccgg aacagagcgt
     5521 ggcgtcaccg agccacaacc gaacttcagc acctgcttcg gcgcgccgtt tatgcctctc
     5581 agaccgggca tctacgctga attgctgggc gataaaatcc aaaaacacgg cgccaaggtg
     5641 tggctcgtca ataccggttg gaccggcggc ccctatggtg tcggcagtcg catgaagctg
     5701 gcctacacac gccgcatgat taccgccgcg ctgaacggtg agctggatga cgtgccgatg
     5761 gttgaggaac cgttcttcgg gttgtcaata cctcaagcaa ttcaaggcgt gccaagcgaa
     5821 gtgctgaacc cgcgcaacac gtggagcgac aaggacgcct acgacgccca ggcccaacgc
     5881 cttgtgggga tgttcaagga aaacttcaag caatttgagg aaaacgtcag caatgagata
     5941 aaagcggccg gacctcgcta ggtaatcggc ctattaccgt ataaaaaacg cccgggggct
     6001 tgcgcatccg ggcgttttct tttgggccta atcaagaaat tttccgcgca gataagcgat
     6061 atagggttcg atcgtcaatg ggccgcctgt cacccgttga ataagctcgt tggccgtata
     6121 cttggagcca tgccggtata tgttaagttc caaccagtca tggagcgttt tgaactctcc
     6181 ctgaccgatt tgggaaggaa tctcaggatg cgccagcaag gcctgctgat aaaacagagc
     6241 cgacatgatg ttccccagcg tgtaaccctg gaagacgccg ccaatcgtcc cggcaaacca
     6301 atggacatcc tgcaagacgc cgtttcgctc ctgttccgcg gagacgccca gatcggattg
     6361 atagcgtgaa cgccaggcgt cgggcaaatc gtgaacgcta agagagcctt caaggagagc
     6421 aagctccaga tcgaaccgga tcatgacatg caagtcatag gtcatctggt cggcctcggt
     6481 gcgaatgagc gattgttgaa ccttgttgat cgccttgtag aaagtctcaa gctctacatc
     6541 gcccaactgt tggggaaagt aggtctgcaa ttgaggatag tagtgattcc aaaacacacg
     6601 actacggccg accacgtttt cccagaggcg agactggctc tcatgcacac cagcggaagt
     6661 accgcccgcc agaggtgtgc cctcatactt catgtcgatg ccctgctcat acatggcatg
     6721 gcccgcctca tggatcgtgc tgaacagacc gttagccaga aaatcttcct ggaaccgcgt
     6781 ggtgatgcgc acatcgccaa gggagaactt ggtcatgaat ggatgatggg tcttgtcctg
     6841 gcggccacgg ttgaaatcat accccagttg acgaatcacc atctcgccga atgcccgctg
     6901 cccctcctca gggaaggtct gatgcagaca actatcatcg acttcaggct gctccagcac
     6961 ttcacgcagg agcgggacca gctgggcgcg caaatcggcg aagagcgcgc gcacgctttc
     7021 ggccttcatg ccctcgtcgg caaaatcgac caatgggtcg gcaacgtgat cgtagccagg
     7081 gaagcaatct gcgattcgac gactcaggtc cagagtcttc tcaagcattg gttccacggc
     7141 ggcgaaatca tccgccggac gagcggtcgt ccaggtctgg tagcttctcg cagaatgctc
     7201 gcacaactcc gacaggagtt gtggtggaac gcgcacggcc ttttcgtatc cgcgacgagc
     7261 aacacgaatc agggcggcat catcatgacc cggatcgagg ctctcttcat aacccctcag
     7321 atcatccagc aggcggccga tcgccggatc gataaatttc tcatgtgcca accgtgacac
     7381 cagcgccatc tggcgaccac gcgcaggcgc gccgccgggc ggcatatacg tcgattgatc
     7441 ccagttcaac agcgctgtaa cgaggttgag gtcttcgacc tcgcgcagtc gatccttcag
     7501 ttcgtccagt ttcttttgca taggagctcc ttaatatcaa atgggcagaa actcatcgtc
     7561 ctgcaggcta tctgccttag ccgtgggtct ggcgcaacgc ctcactcaga agaaatcgca
     7621 atgcgggcgg caggcgcctg gcccaggatg attcactatg ctcggcccac ttttcttcaa
     7681 cgtgcaatat atcgcgcaac ggccggtagc ccttgcgcac aagcatccct ttcagccggc
     7741 gaacgctggc ataatagcgg cgcgaggcag ccgcgcgatg caaacggccg ccgccatggc
     7801 tctcgccata ttcacgcgtg cccgcatcca gatagatttt cccggggtga aaagaggccg
     7861 catcgatgta gtcaaagatg gccccacttg caaaccacag cgacggactc atcactccag
     7921 catagccaaa gacggtggga tactcaaaaa acgcatagag actgatcagc cctcccatcg
     7981 atgaccccag gatgccggtg tacttgggtt ccggcaacgt tcggaactct ccatcgatct
     8041 gcggcttgat cgtatcgatg ataaatctca ggtaatcatc gcctcggccg ccgcccagtc
     8101 gggcgtcgcg aaaaggacta tactcatcaa gtcgatggat tcccgcgttg ggaatgccaa
     8161 cgatgatagc ttccagccct tcctctctcc ccaggtgctc catcgtctca tctacgcccc
     8221 actcgccggc atagctggtg tcactatcaa agaggttttg gccatcctgc atgtagatga
     8281 ccggataacg ccgctcgctg tgatggtatg atggcggcaa atagacgagc agatcgcgct
     8341 tgttgtccag ctgtgggctg tagacaccaa gcgcgatgcg aatgcttcca ctcacactat
     8401 gcccacgcga gtggctgtaa taagagcgat aatcttgcca atccaaagct agttctttgg
     8461 taatcgatcc ggtattcatt tccattaagc aaaccagggt tgtgcctttg atgacacaac
     8521 cccatgtaat tctgcccatt ttattggatt tgtccaatca ctttaacgtt taacaacgac
     8581 atgcctgttc tataaatcat atctgttggc ctaccccctg tactcctcgg aaaaaattag
     8641 ccgtctctgc agggttggag cgacgtggcc aaccttgtag aatcagacca tgaacagttc
     8701 aaacgatgac gagcgacgtc aggaagaaga gagcggctcc ctggatgaac atcagcgacg
     8761 catgcgattc caatggaatg acttgattga ggacctaatc gaagacggcc gccgtcgtgg
     8821 gctgtttgaa gacttgccgg gcaagggcaa gcccctcaat ctcgaacaaa acatcttcga
     8881 aggcaacagc acgctggcca atcaattgat gagagccaat gacatcaagc cgatctggtt
     8941 ggcgcagcgt atcggcgtgt tggagaggat cggcgaattg cgggcagaca tggcgcgagc
     9001 atgggaacgc tacgacatcg ctttctccca cgtctatggc gatagtcatc gacaggcgct
     9061 cacaatcggc tgggatgagc aatgccaacg ctggcaaaaa acgatcgaga agctgaacaa
     9121 agaaatcggg acctacaacc tgaagcgccc cgacggccaa cctgaactca taaagctgag
     9181 gctgaatgat gagctggagc ggatcgatgc accacgatac cttttataat ggtcggccca
     9241 tcatcacctc atctatttca cacgattagc tgaatatcta acatcgcagg taagattatc
     9301 ccccgcacgc atcgtcagat gaacaactga tagctctgct caacttctat caacccatca
     9361 gttctatatt cgccgatcgg tcggtctttg atcgacccga tcgatattgg ctgttatgca
     9421 tttatatggc gttggagcca ggctgcttac taaatattgg agcttccttc gccgatacat
     9481 caccgatcca acagctatcg ttcatgggat caggataatg acaacaacgc ttgttgataa
     9541 cacggaatat atttcgccaa atgaacgcct cgactacttc cggaaaatag actcagcaaa
     9601 catcccatca gttacaagaa agctgattga agagcctcaa cataatgacc catcgtcctg
     9661 gttagaaggt cctgatcgag gcaatccacc caacaatctt gaggcgattg aagatcacga
     9721 aaacatcatt gcaattgatg agacgggtaa cggttctgat gaggccgagg ccgaatcgtt
     9781 cgtcgatgtg gaaggctatc ttgatgagta tgatcgccaa caagccgggg acaaatacga
     9841 atcactgcaa aagctcaccc aacaacgacg ttcggccctg gacgacgtgc cgacggccga
     9901 tttcctcagt cgctatatgc tggaaaccaa tcgacagcgt ctcctgaccg ctgaagagga
     9961 agtggagctg gcaaaacaga ttgagcttgg gaactttgcc gctcaacgcc tgaatgaaga
    10021 ggatatagaa gccgaggaat tggcggaact gcagcgaatc atcgatcagg cagacgcagc
    10081 gcgcacgctt ctcgtccgct caaatacgcg cctggtcatc agcatcgcca aacgctactt
    10141 tggccagggg cttgactttc tcgatctcat tcaggaaggc aacatcggcc tcctgaccgc
    10201 ggtgaacaag tttgattaca cccgcggcac gcgcttcagc acctacgcaa catggtggat
    10261 tcggcaaggc atcacgcgcg cgctctcaaa ttacggtcgc atgattcgca tcccggccca
    10321 ccagaccggc aacgtgcgca agctctatcg cgccattcgg gatatcgaac aaaatcaggg
    10381 gcgacaggcc gacgtcgaag agttggccca atacgttgag ctgccctcaa gtcaggttcg
    10441 ctggctgctg cagattactc ggccgctcct ggccctggaa caaccggcgg gggatgatca
    10501 ggacgcggag ttagccgatt tcatcgaaga tcgcgacgcg gaacaaccgg cggatatcgt
    10561 cgatacaaaa ctgttcggcg agcaggtcgg cgaaattctg gccgaactca cgccccgcga
    10621 ggcagcggtg attcgcctgc gctatggcct tcagggcaca gatgcccata cgctgaagga
    10681 agtcggcgag atcttcaagc tctcgcgcga gcgcattcgt caggttgaaa aatcggcact
    10741 gagcaagttg cgccacagcc acagcctggg tgattacgcg tacatgatga actgatcaat
    10801 aaaaaagagg cgcatgtgcg cctctttttt attgatggcc gttgctgctg ctcacagcct
    10861 gagccagcac tttcatccgt tcaagctccg catacatccc ttccaggact gtgtcgggat
    10921 cgggaaccag tccggcatcg gtggcgatcc ccagataaac attcccggca tagcttagaa
    10981 tgctaacccc cacacccagg cggccggcct ggggaaccca aaacatgatt tccttaatct
    11041 tttggccggc catatagaga gggatcggcg ggccgggtac attagtcagg acaatggttg
    11101 ctttcggttc gagtatgcgc acaacgagtt cgcggaaccg atctgtgccc atgcccatca
    11161 tacccagaac gccgagcgtg acgggggcct cggccgatcg ctttaaggct tccatccgcc
    11221 gccgaacctc cgtcaatcgc atcagaggtt cctcaatttc aatgggcaac gtgaggaata
    11281 ccaggccgaa cttgtttccg agcttgccgc catcttcctc gccacgcaaa ttgacgggga
    11341 tggccgcgcg aaactccaga tcggcgacat catccccctt ggccaccaaa tagcgtcgta
    11401 atccgccggc aacgaccgta acaagaacgt cattcaccgt gccgcccaga ttgcttttga
    11461 tcaacttgat atcagccagc ggaatggcgc cggaccaggc ggcccgcttc atgaccccca
    11521 actttccctt caagcgcgtt ttggggtcag cgctccgagc gagcaggcgc gtagccgcca
    11581 aacccaggcc cgctccctgc tcgccgattt gccgcgcctt atcggtatcc tgaaccgccg
    11641 aaacggcccc ctgtagggcg cgatcggccg ttcgacgaaa caaatcaacc gtactcgtca
    11701 tctcccgtgc caggccggat atgaggccgc catcgggcaa aggattcatc gtttcagtcg
    11761 ggacaagagg cgcgtcggca taaaaatcag tcatcgacag gaggacggcc accaacgcca
    11821 ttccgtccgc caaagcatgg tgcaggcgcg ccatgactgc gcaaccttcc ccgtaattct
    11881 caatgatata gaatttccat ggcggccggg aaaagtccat cggcatgctc atcagatcgc
    11941 tcaccatgtc ctgcagggca gcttgatcgc caggcgcagg aagggcaatt cgttgcaaat
    12001 gcatatccag attgaagttc gggtcggtct cccaataagg ccggaagaag ggaatggcgg
    12061 gccggataac gcgctgcttg aaacgctcat actgtaacca gcgatattcc agcacttttt
    12121 tcacccgatc gatatccaat ggctcatcaa acatgaagac tgcattcacc atcatcaagt
    12181 tggtcggctc atccatgctg agccaggcca cgtctacggg ggacattatc tcgatttttc
    12241 cggccatgcc ttctctctcc gggagcactg atacatctta ttgagcgacg aattataacc
    12301 ccatttggcc aggatatggt actaacagat cacgcgcccc atcaactcaa tttgccgcca
    12361 agcgtgcatc tgctataatt cagtccacaa tgccacccta gctcaattgg cagagcaatc
    12421 gcttcgtaag cgataggtta tcggttcaac tccgatgggt ggctcagaag gttagccctg
    12481 gaggagccag ggctttttta ttgttgagcg gtgcctgatt ccttcgcaat ggggtcaatg
    12541 aacgtaaaca ggatgagttc tcctaagtat aaacccgtcg tgttcggtgt agccggcggg
    12601 accgcatcag gaaaaaccac agtcgcccgc gccattctcg acgctgtcgg cgcatcacag
    12661 atcgcctatc tgccccatga tgcttactat catgatcggg tggatctatc gttcgaggac
    12721 cgtgcccggc tcaattacga tcatcccgat tcactggaca ccaagttgct cgtccgccac
    12781 atcaaggatt tacgcatggg ttcccccgtc cacgtaccgg tctatgattt cacgaccgac
    12841 cggcgcactg aggatacgat cctggtggaa ccggcgccga ttatcctggt cgatggcatc
    12901 ctgattttca ccaagcggaa gttgcgcgat ctaatggaca tcaagatatt cgtggacacc
    12961 gacgccgatg tgcgatttat ccgccggttg cagcgcgata tgaaggaacg cggccgctcc
    13021 ctggattccg ttgtccagca atatcttgaa accgtgcggc cgatgcactt gaagttcgtc
    13081 gaacccagca aacgctacgc cgacatcatc atccccggcg gtgggcaaaa taaagtagcc
    13141 atggaaatgg tcgtcagccg gctccagatg cttttgaaag ttcgcgagga gcaagctgaa
    13201 gaggagattg cgtgatgatc aattggagcg aacaacgagt agtcgtgacc ggcggatccg
    13261 gttttctcgg ccgccatgtt gtgggtgcct tgcagcgccg cggtgcaggc aacgtcgttg
    13321 tgccgctgcg cgaagattac gatcttcgcg accagggcgc catcctcgat ctgttaaatg
    13381 acgcgcggcc cacgatggtc attcatttgg cagcctctgt gggcggaatc ggcgcgaatc
    13441 tggcgcatcc ggccgagttt ttctatgaca atctgatgat gggcgcgcag cttctccatg
    13501 agagctggcg gtccggggtc gacaagttcg tggctattgg gacggtttgc gcctacccga
    13561 aattcacccc cattcctttt cacgaggaag atttatggaa cggttaccct gaggaaacaa
    13621 atgcgcctta tgggctggcc aagaaaatgc tcctggtaca gagcgaggcc tatcgccagc
    13681 aatacggcta caactctatc ttcttgctgc cggtgaacct ctatggcccc ggtgataatt
    13741 tcgatcccga gtcttcccac gtcatcccgg ccctaattcg caaatgcctg gaggccagac
    13801 agcggggaga cgagcgtatc gtcgcctggg gcgatggctc gcccacccgc gagttcctct
    13861 atgtcgagga cgccgccgag gggatcgtca tggccggcga acgctataac caaagcgatc
    13921 cggtcaatct cggctcaggg ttcgagatca gcatcaagga tctgctccaa actatcgctc
    13981 gtcacaccgg cttcaccggc caaatcgaat gggacaccac caagcccaat ggccaaccgc
    14041 gtcgcgctct aaacaccgat cgcgccttcg aatatttcgg ctttcgggcc acaaccgact
    14101 tcgacgaggg tttgaacaag actgtggagt ggtatcaaca aacgatcgcg gattcatcac
    14161 agacattagt caggccgcag tagggatgcg gggagacgaa ccccgatgag cctggcgcag
    14221 cgaagcatca cctccgtcgc ctggaacgtt ttcgccaatc tgttcaagat cgcgatcctc
    14281 ctcgtgcgat caatctggtt ggcacgtttg ctgcccgtcg agaccttcgg cgtctacgca
    14341 ttggctacct ccatcgtcac cttcaccggc gttataccca tgtttgggat ggcaagcgcc
    14401 tttctgcatc gcagtccgga gacggccaac gaagagcaag ccgcggccgt ccactttacg
    14461 ctgcgtctgg tgttgacatc ctgctgggcc ggtatcctta tcctcgcggc atttctattc
    14521 accagcgccg aattgcgtct ggcgctcatt gtgctgaccc tggtattttc aggcctctat
    14581 ctgacggaca cgccaaaggc tatcctgacc cgccgggttg agcatcgtcg gctggccatg
    14641 ctcgacctgc tgacggcgct ggtgacctcc atcgtagctg tatatcttgc cgctcgcggg
    14701 tttggcctta cagcccttct ggccaccgac atcgtcaccc tcattctggc cgtgatcatg
    14761 ctttatctgt ggcagcccgt ctggcggccc cggctcctgt ggctgcgcgc cacggttcgt
    14821 tattacctga ccttcggcgg ccgcgccatg ctgggcggtc ttctcagcga cgcgctggat
    14881 catttggacg acatctgggt gggtgctttc ctggggaacc aggcgctggg cttctactcg
    14941 cgcgcctaca cattcgccac ctacccgcgg cggctgatcg cctttcccgt gaacatggtc
    15001 gccggcggca cctatgccga actcaagagc gatcgcctca ggctttcaca agcgtttttc
    15061 cgaacgaatg cgctgctcgt ccgcagcggc tttttgatcg gcgggttact cgtgctcatt
    15121 gccccggagt ttgtcagcat tgccctgggc aacaagtggc tgccgatgat acccgccttt
    15181 cgtctaatga ctgtcttcac cttgctcgat ccgatccgca ttaccgtcag tcaggttttc
    15241 ctggcggtgg gccagccgga ccgtatcatt cgcgtgcgaa ctgtccagct catcgttctc
    15301 atcgccggtc tgttcgcatt cggcctttcg ctgggaatcg agggggtagc aatcgtcatg
    15361 aacctcgtgc tcctcgcggg gcttgttccc ctgctccatg cagcccgcga gcatgtcgat
    15421 ctgtcggtat ggcgcttgtt tggcgcgccg acaattgcct tgatcattgg cgtagccgcc
    15481 gctctgggca ttacttattg ggtttgcctc agttccacct gccccaatca ttggatcaca
    15541 ggcttcagca aatctatcgt attttcgttg gcgtttgttg ggacgatcgg gtcactggag
    15601 gggaaagagg ctttggaact tatcgggcgt gtaactcaaa tttggcgcgg caagccgatg
    15661 cccaacccag accaaccttc aagcccggaa tgaatatcac agtcggcatc acctcctaca
    15721 atcagagcgc gttactgcgc gtggccatcg agagcgtact gagccagacc aggcaaccgg
    15781 ccgaaatcat catcgtcgat gatgcttcta ccgacgactc caggcatttg atcgatagtt
    15841 atgtcaataa gtaccccgat ctaatccggc caatctttct caatcacaat ggcggcccca
    15901 acaacgcgcg caatcaggtc attgacgccg ccggagggga atttttgact ttcctggatg
    15961 gggacgaccg ctggctggcg accaagctgg agcaggagac gaaacggctg accatggccg
    16021 atcagccgga cgcggtgttc tccaacttct acttcacgga tggcgcgggt aatcgcgagt
    16081 tcatgtgggt gagtgattcg acgcctcggc cgccggaggg ccacatcctg ccccaggtgc
    16141 tggccctcaa cctgccgcga aacacgctct tccgctccga gatagttccc acccgactct
    16201 ggcgcgaggc aggctacttt gactcgtcgt tcacgatcta tggcgattgg gacatgcgag
    16261 tcagactggc agcggcggtg ggacgattcg gttatctgga tgaaccgcta tgcgaatatc
    16321 gccgacacgg ccaaggcttg tccaacaaac cgatcgagat gcatctggca gccgtcgacc
    16381 atatcgagca gaaatatgcc gggctgatct cgcagctaaa tgagactact cgcaaatcgc
    16441 cgatcagggg actggacaaa tttcgcgcca agctgctgcg gcgagccgcg cttgattcgc
    16501 tcttgctgcc atctaccccg gcgcgaaaac aagcaatccg atattatcaa cagagcttgc
    16561 ggtttgaccg ggtgctcgat tttcgtttat tctggtatct tttgaaggca tgaatctttc
    16621 tccccctaca ttgccggacc cgcttgagcc gtcgaacccg caggatgctc gcgattcttg
    16681 gccatggctc gccccaccta tggacgccgc caacgttccg atccgtgaag gaacctggcc
    16741 aaaaatcacc atcattactc cctccctcaa tcagggcaga tttctggagg cggccatccg
    16801 ctcggttctg atgcaaggtt acccgaatct ggaatatatc atcgccgacg gcggctcgac
    16861 cgatgacagc ctgaagatca tccgtcatta tgcgccattg ttgtcgagtt ggtccagcga
    16921 accggacggt ggcccggcag aagcgctcaa ccgtgccttt tcgcgcgcaa ccggcgatat
    16981 tctggcctgg ctcaatgccg atgacgtcta tttgcccggg gcacttttcg ccgtggccga
    17041 agcgctggaa cagcacaatg acgccgctct cgtctatggt gaaggctggt atatcgacga
    17101 atccggccgg cttatcgaac catgtcgttt cgtgcgccgc accttcacac gtcaattcct
    17161 ggtgaacaaa gacccgatcc tccagccggc ggcattttgg cgtcgcgagc tatgggaacg
    17221 gaccggcccg ctggatacga gtctgcgttg ggtttttgat tgggaatggt tcattcgcgc
    17281 ccatacccac ggccgctttc aatacatccc ccgtgatttc gcctattatc gcgtgcagcc
    17341 caatgccctg acgcgcacgg gcggattgcc gcgtcagatc gagcacggcc gtgtcactcg
    17401 ccgctatggc acatggtggc atcccaatca tctcgtccag caaacccggc ggctggacgc
    17461 agtggggcag aaactaaccc acggatggcc gcgtgttcct gcggcgattt tccgtacgcc
    17521 atttgcgata ccgcgatacc tggcggaatg gctgctccat ggcatgtaca tgcggtaaca
    17581 tgtaccagtt ctaaaatcat ccttttcatt atgaacgtcg ccttcctgcc tgtctatcct
    17641 aacccctacc agaagctgct aggcaatgcg ttggcggccg aaggagtgac ggttgacctg
    17701 ctggaaagcc ttcctgataa ccaatggcta cgggacaaca tcggccgtgt tcaaatcctt
    17761 cattatcatt ggctctccgg cctatatatg aaccggtttc tcacgccgct tcaggtcaga
    17821 aaattttgcg cccgtttccg gctggcccgc gactttggct atcgaatcgt atggacagcc
    17881 cacaacattc tcccccatcg cactttcctc gctccgctgg acaaggcgat tcggcggctg
    17941 atgatggctg aagcggatgt cgtgatcgcc cattgcgagt acggccgccg cgaattgctg
    18001 gcccggtttc cgcgaaaagg accggtcgtc gtcatccccc ttgggcatta ccgggatgca
    18061 tatccggcca ccctggatcg ccgaagcgcg cgcgaacggc ttggcctcca ccccgatcaa
    18121 ttcgcctacc ttgccctggg gaatattgcc gcctacaaag gattgagcga tctggtgcat
    18181 gccttcaagc aggtcgcagg aataaatgac gtgctgttca ttgccggacg aaaccgcgac
    18241 caggacgtta tatcccggct gctgcaagcc gcgcagaccg acagccgaat tcatatccag
    18301 ccacgcttta tccccgatga tgagatgcag atctatctct tcgcggccga tgcgatggtg
    18361 gcgccgttca ggcgagtgct cacctcaagc tcggttatga ccggtctgtc cttcgatctc
    18421 ccgcagatcg tccccgctct ggggtgcctg ccggaattgg taacgccgga cgcagggatc
    18481 atctatgacc cgcaggacga aaacggcctg gctaatgccc ttaccgacat taaggttcgc
    18541 gatcacgacc aaatgcgagc cgcggcacgg cgcatcacgg atggattagg atgggccgat
    18601 atcgcccggc agaccgccga tgcctacagg agttgcctca cgtcatgaca ggcagtggcc
    18661 aaaaggttgc catcctctct tccgtccacc tcgccatgga caatcgcgtc ttttatcgcg
    18721 aggcacgaac gctggcacag gcgggctatg atgttacgct gatcgccatc cacgatcgga
    18781 acgaagagat cgatggcgtg cacgttcgcg cattgccgcg cgtgccgcgg agccgacggc
    18841 cgttgctctg gcggcgtctg gcgcagatgg cactggagac cgaggccggc atctaccaca
    18901 ttcacgatcc cgaactgctg ctcatcacgc cttaccttcg ccggaaaagc gggcgcccca
    18961 ctatctacga catccatgaa gccaacgccg acttcatcgc cgtcaaggat tacttgcccg
    19021 ggctgattcg attgccgctc tcagtcctgt tccggcaact cgaacccatt ctggcacgag
    19081 gtgaggatgg tttgattttt gccgatgact ccatcgcggc cgattttgaa tccgttcggg
    19141 ctattaaaac gaccctgttc aatttcccgg gagacgaatt gatcatgcgc gggagccttt
    19201 gcccgccgcc tgatcctgac gtgtctcccg tcattctcta tttaggcggg atggaacgga
    19261 atcggggttc ggcgctgatg gtggctgcct tcgccgaggt gctggaaaaa aggcctgacg
    19321 cacgtttact cgttgtgggg catttcatgc ccgccacgct ggaagacgag atacgcgccg
    19381 cggccatcgc ccacaatatc acccacgcgc tgactatcac cggccgcgtc cccttccaga
    19441 aaatcgggca atatctggcc gaggcgacga tcggctgggt aacctggcaa ccgttcgcca
    19501 agaacgagag aaacatccca accaagctat tcgagtacat ggccttcggc cgtccagtga
    19561 tcagcagcga tctgccctca accaggccgt tcgtagagga tggagtcaat ggcttccggg
    19621 tgaaggcaga tgatccgcac gcacacgccg aggccatcct tcggttgatc gagaatcctg
    19681 ataatgcgca ggcaatgggg cttgcgggca gagagatggt gcaaacccgg ttcaactggg
    19741 gcaggatgat cccccgcctt ttggagattt acgacaaggt gttgtcgtct cgcaaaacat
    19801 agcccagccg ctccatcatt ggttcgatga tgggcgctat ccgtgcgatc atttcagcct
    19861 cgcgtcgcca tttatccaaa tcgggcgcag aaacggcgtt tacctccggc agaggaccgt
    19921 cgacagcggg gatcagatca ggcgacagcc cgagaaattc gctgacacgc gtcagggtcg
    19981 gggccggagc cgcaacaaga tcctcaaatc gaatcgttag cgcgggcaga gcgcccggcc
    20041 ggttggtgta gtcgagtacc gcctgattgg aagcaaccca ttgatgagcg gaaatcactt
    20101 ccagagggct actcaccaga tcacgccagc cggggatcag cgtgaaggcc cagcggcctc
    20161 gcgcttgccc ggcgctggtc acggccacgg gcgttcgata gccggggaag aggtggggct
    20221 gtcgccagcc ctccaccaga gaatgaatgt tcgctcggcc gtctcgcgtc agatagatca
    20281 ctcgcgcatc gggaaagagc gccgccagga acggcaagcg caggcagttc tccggcgtct
    20341 tttccagcaa gcgaacaggc gcgcccggag gcatcagtcg gtttcgcaac tcgaccaccc
    20401 ggcgaaaaaa gcctaacccg gcacccggca ccgcgctgct aaacgatgcg ccctgagaac
    20461 cctgccttcc atgccgcttg actgctctgt agaggccatt gccgttcaac gatcggcgca
    20521 accgattgac ccgataccag taactgccgg gagcagcctg ttcaagaaat tgtcgatgga
    20581 taaacgcggc cgattcggcc gtcaaatcct cggcaaccaa ctcgccggat acccaatcct
    20641 tcgtcacggg agcatgatag cgctcaataa tagccttgct ctcgccacca atagaccata
    20701 gagaaggctc ttccgacaac aggttgtaga gcaaggtcgt tcccgaacgg gcacatccga
    20761 taatcaggat tggcgaagaa atcataaggc agctatcata ccgtcaggca cgttcgcagc
    20821 gccaccctaa ctttcctgga gatgcgcgtg cccgacgtgc cattgggtga accatgcgga
    20881 ttttgtttct gacaggtcga ctgccctatc ctccgaaccg cggcgatcgc ttgcgcgtct
    20941 atcatttcct gcgcgtcctc tcgcaacaac atcaaatcac cctcctgtcc ttcatcggcg
    21001 acaatcgcga aacaagtttc atcgggccat tgcgccattt ttgcgaagat atccagctcg
    21061 tccatcgtgg agagcttcag tcaaccatgt cgacgggatt aaacgtctgg cgaccaatgc
    21121 ccttccagtc gctttattat cgctcatcgg tcatgcaagg cgctgtggat cgattgctgg
    21181 atcggggacg attcgatgcc gtctatgtac acctgtttcg catggctcaa ttcgtcgcgg
    21241 gccgcacaca accctatcgc atcctcgatc tgaccgacgc tatatccagc gaagttgaga
    21301 ggtcgctgcc ctatcgcgat ccgaaatggc ggctggtcta tcggcttgag ctgccgcgca
    21361 tccggcgcta cgaacgtgat atcgtgcgtc actttgatga gacatggctt atctccaatg
    21421 ccgaaaagca gaagttatcg gcagacaatt acaacgacaa gattcaagtc gtgcccaatg
    21481 gggtagacag cgcccgttat cggccgtccg gcctcccggt cgatagcccc cgattgattt
    21541 tcgtcggcca tatgggcgtc tttcacaatg tggacgcggc ggagcacctt gtgcgcgaca
    21601 tacttccccg cgttcgcgcc atattcccca acgcaagtct ggacctggtc ggcgccgaac
    21661 cggcgcctgc cgtgagacat ttggcatcta ctcctggagt acgcgtatta ggtcatgtcg
    21721 ccgacctgaa cgccgcgctc aatgatgctt ctatttttgt cgcccccttg cgcttcgcgg
    21781 cgggcgtcca gaacaaggtt ctggaggcaa tggctgtcgg ccttcccatt gtcactacaa
    21841 cttacgttaa cgacggtctg gaggcggaag acggccgcca tttgctggtg gccgatgaac
    21901 ccgaccaatt tgccgatgcc gtcatcacac tgctcaagaa cacccagtta cgacgaagcc
    21961 tcggccgcgc cgggcgggag ttcgtaatga accattatcg ttgggaagat gtcctggatc
    22021 gggtgaacct catccaaaat cagatcgccc atcaacaacg acgctagatc aatgacctgc
    22081 ccgaccctct ccccactgat ctgccatatc cgtttccaga gttcccaaaa ctgtgatatt
    22141 ctatggccca atgaactcca ggctctcacg atggtgcgac ggcttccttg aagccggttg
    22201 gttggtagcc attctggcta ttcctctgtt tttcaatatc cactccgagc gtgtttttga
    22261 accggacaag ctgactttgc tgcgttccat cgccgttctg atggctgcgg tttgggtcgt
    22321 gcggttcatc gatcaacagg gctggcgcgg tctggccgag cagcgattct ccaatcccaa
    22381 ttcgatctgg cacaagccgt tcgtcctgcc ggtcattctc ctgctgctag tctatttgct
    22441 ctctacgctc ttctccatca atccgcgcat cagttgggcc gggtcctatc agcgcttaca
    22501 gggaacctat accacgctgt cctacgcggc aatattcgct atcatggccg gcaccttgcg
    22561 cacacgcgag caagtccggc gcgtgattaa cgtggcaatt gtgaccagca tcccggttgc
    22621 tctctatggg attctgcaac atttcgggca cgatcccctg ccatgggggg gcgatgtgca
    22681 aacgcgagtg gcgggacaga tgggcaatgc gatcttcatc gccgcttatc tgatcatggt
    22741 gatccctctg acgctggctc gcatcatcga cgccttcagc aatatcctgg gggacgaacg
    22801 gcttgccgcg gccgacgtag tacgagcttc cgtctatatc ttcacgctgg ccatccagct
    22861 tctgacgctc tattggtcgg gcagccgcgg cccattgatc ggcctcgctg ttggcctttt
    22921 ctcttttctg ctggtgctgc tcgtctcact gcgggatgcc acgaaagatc aggcgaccag
    22981 ccgggttaag gaagcattgc cggctttggg ctttctgctg ccggcaatat tggctctatt
    23041 gatcagcccc atcatcagcg ccacattcgg ccctatggcg tcatttggct tcttctttgg
    23101 cgtcgtagct ctgtcggttg tttcaatctt tctgatggtc tcaattcgtc gcggatggcg
    23161 ctggctttgg ctcagctgga tcttgttgac cgtgctaatg gcgggctggc tccttctctt
    23221 caatatccca tccgagcaaa ctgccggctt gcgtggcgtt cccgtggtgg gaagtctctt
    23281 caatacgctt gaggaatggc gcaatctgcc ggctgtcggt acctatggcc ggatgctcga
    23341 cccgacggac acaaccgggc gcgaaaagag cggccgcgtg cgtgtgctca tctgggaagg
    23401 ggtcattgac ctgatcacac cccacacccc gctggaatat cccgacggaa gctcagaccc
    23461 attcaactgg ctgcggccgc ttcttggcta tggcccggag tcgatgtata ccgcctacaa
    23521 ccgattctat ccaccagaat tagctaccgt cgaggcgcgt aatgcttcgc ctgatcgctc
    23581 ccacaacgag acattcgatg ccctggttat aaccggcctc gccggtctac tggcctggca
    23641 agctttatac ctgtccgtgg tgcacttcgc ctttcgctac ctgggcgtca ttcgttcgcg
    23701 ccgggacacg tgggtcctca tcggcctctg ggtgacgggg gcgttgcttg ccactctcgc
    23761 cacaatcatt atcaatgagc ctgtttatct tggcgtggct gtccccattg gcgtgattgc
    23821 cggcgttatc atctacctga tttactacgc cctctttggg agagaccaaa gtgtggttgc
    23881 ggctgagcca agcatcgcgg cattcctccc cttttcggcc gaccgactgc tcatgaacgg
    23941 gctggtcgcc gcggtgttgg cacactatgt agagattcac ttcgggatcg ccatctccgc
    24001 cacgcgcctg tattttttcg tctatatcgc gctgatgttt gccctcggtt accggttgcg
    24061 ccagccggaa ccatcaaccg aagagatgcc tgccgtcgct ccagttcccg cgaaaagcag
    24121 caagcgcaac cgccggacga ccgtcgtccc caccgaaccg acgatcagag ccgggtggca
    24181 gcggctcatc gtcccggcgc tcctcctgat actcatgctg ggcctcttcg gctatggttt
    24241 tatcacctat gcattgccgc cggataaagt aatcaccggc cccgcggatt tgacggccgg
    24301 agaaatattc cgccaatctc tttttcagaa ctcgcgcaaa ggttttgtcg attggccttt
    24361 cgtcacgtcc atgatcattc tgtcgtgggc gctgggctgg ctcgtctttc tcagcgagat
    24421 ggccaagtat ggcgagatca agctcgcttc agcagggagc atagccggac gggggcgcgc
    24481 cgcggcgggg gtgttgttcg ctctggccct ggcgggagca ggggcacgat tcatcatacc
    24541 ggcaggcagc atcacgacga cactcggctt gagcctcggc ctgatcgccg cgctcgcctg
    24601 tgcgttagcc gcagttctgc tcatcatgaa aaggccatca ggacggctag cgggcggcat
    24661 cacggccgcg agcctgatca tattcgcgtt tcccctattg atagcggggg caatcctccc
    24721 ggccacaatg atgatcctgg gtggcgcgat cgtgctctgg ctgctgtggg acggcaattg
    24781 gcgccattcc ctgttgccta tcgcgggcgt gattcttgtc tcactcctga ccggcttcgc
    24841 ctatatctat gttcatgcga tcaactatcg ttcgatgctc ttttatcgcg gcagcgagca
    24901 accggcctca tcgaccatgc tgcgggtgtt cgaagcgtca caaacatcgg aattgatcgc
    24961 tttctttttc gttttcgtat tcatcatgct gttattgctg gcctttgccc ttagctggtc
    25021 ggggttgacc agcgggcgtt tgccacgaac caatgtatcc cccctgccgg ccttcgggac
    25081 gctggctctg gtcttcatcg cggccttttt catcatctcg caatcgaacg tgcgtcaggt
    25141 gcaagctgat atgatctaca agcgtgcccg accctatgac gatcaggcgg cgcgctccag
    25201 tcaggcaaga cctgaagtac gccgcgacct gtgggatacg gccatcgcca tctatagcgc
    25261 ggccatcgac cggatacctt cggaagattt ctactacctg ttcctcgggc gtgcttacct
    25321 ggagcgcgcg gcgctcactg aggatccaac agaacagacc gagttgctct cctcggccga
    25381 gaccttgctg ttgcaagcgc aggcactaaa cccgctgaac accgatcata ccgccaactt
    25441 ggctcgactc aacacgcgtt ggtatgcagc cgttggtgac ggatccgaaa aagccaaacg
    25501 gctcgatctg gccgaaagct attacgagga cgcgctaagg ctcagcccgc aaaactcggt
    25561 cattcgcaac gaactggccc gccttgtcct ggagctgcgc ggcgattgcg atcgcgctct
    25621 ggcgctctac gacgaaagcg ccaccattga tcccttctat tcccagaccc aactggcgcg
    25681 ggccgatgcc tatattttgt gcagcagcgg ccgcacggat gcggaacgag acactttata
    25741 tcgggccgcc gccggtgcat tggaagaagc gttagcggat gctccctcca atatccgtgg
    25801 ctgggtccaa ctggctgaaa tctatcgaca actgggcgag ttcgaccaag cggcaacggc
    25861 tgtggaaaac gctcgcgcca ataatggcaa agccaccttc cccccttcgg agatcgattt
    25921 catggacgcg cagatcgccg ccggcctggg cgatctcgac aaagccagga cattggccga
    25981 acaggcattg aagacagcca ccccgaatac ggccgaacaa atcgaggcat ttctggcgga
    26041 gcttggcgac tgactaatgg gaacggcgat gtaccagctg gtcgacttct cctgtccttc
    26101 ttgattatct gctcatgcgc gcgtttcttg ccgtctttct catcgcatta acgatcacgc
    26161 ttggcagcct cccctgggtt cgtcgccttg caatcgccat gggttttgtc gacatccctt
    26221 cccaccgcaa ggcgcatcgc acgccgacac cgttgctggg tggcgcggcc attttcgtcg
    26281 gcgccatact cggcgtgctg atcatctatg g
//
LOCUS       DAOMQG010000011        26120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_813_length_26120_cov_11.631345, whole genome shotgun sequence.
ACCESSION   DAOMQG010000011 DAOMQG010000000
VERSION     DAOMQG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 26120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26120
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_813_length_26120_cov_11.631345"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLC06_01520"
     CDS             <1..553
                     /locus_tag="PLC06_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89152.1"
                     /translation="LRGQHIVPLPASLTSGRYRFFLGDFPLEEITVIAPERSFTQPEI
                     SQSIDAAFATADGAPVATLVGLAVDSSSSSCSPAPLLPCALPLVWRADGEASAGYHAF
                     VHLVDEAGNILAQSDAVPAGWTRPTTGWLPGEYIMDVHTLTLPPELPPGQLAFRAGLY
                     DPVTDERLSVNGTDAVLITLPGS"
     gene            complement(569..1267)
                     /locus_tag="PLC06_01525"
     CDS             complement(569..1267)
                     /locus_tag="PLC06_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89153.1"
                     /translation="MFRFKALLLVGVSAILLAGLIVFTRSTSADNPYPPVVIETREIP
                     EGIVTESMEEVDSPFPNLEGKPVKVYTNVIEVPEGTITEQIEVIDPLPTDTQDAQKDV
                     PFNTDDLLQIERIEFNNDTGLIDLVIDEPQSKTPTLDKKGNPNPLSWSIFADDLYATC
                     SAGAKFDYSSTTGTSYYTLRWNGGQTANSKTCSGAQCNYIISTSWSDYYTYADVQILS
                     PALVWGPSNAYRCK"
     gene            complement(1349..2530)
                     /locus_tag="PLC06_01530"
     CDS             complement(1349..2530)
                     /locus_tag="PLC06_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRO89154.1"
                     /translation="MNKLHQRLRLVLTLRLRLTLWFIVLLAITSIGFVLAVNVVAAIL
                     IPQTVSDIPLQDFASEESDDPSVAPNSSSLPSIQIPRRNSSLIIDLTLQGVRAISLVV
                     MGFMIILGSAGAYFLAGRALRPISYLSQEIAEIDSGNLGKRLSVEGAVDEVSRLADAF
                     DNALERLDYSFGQQRRFASNAAHELRTPLAALRANLEVILDDPRATLADYQEMAITQE
                     QMLARLERLVSNLLLLTRGEYESVDKEISLLPLLEEVVANLSPQAEKYQVSLRLVAQT
                     ESFLNGDDVLISSIFSNLIENGIRYNRPGGSVEVSAWEEPAWIVISVADTGVGIPAEE
                     QSLILEPLYRRPVPSSGHDPGTGLGLSIVAHLVHLYRGRLDIESTVGVGSTFIVRLPK
                     T"
     gene            complement(2564..3265)
                     /locus_tag="PLC06_01535"
     CDS             complement(2564..3265)
                     /locus_tag="PLC06_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89155.1"
                     /translation="MSAEEVGTNVMRLLIIEDEVVLAQAIAKGLRRQGFAVDIAANDE
                     ESQEFTGVNEYDLLILDLNLPGADGLEICSRLRAEKPELLILILTARDRLSDKIAGLD
                     SGADDYIVKPFHFEELSARIRALLRRDLRVREPIRSVSDLKLDPAAQIVWQEDRRLEL
                     TRKEYGILAYLMSRPGEVISQEELLEHVWDSSVNPFTHTVRVHINSLRRKLGDDPQTP
                     RYIETVAGEGYRLLT"
     gene            complement(3287..4189)
                     /locus_tag="PLC06_01540"
     CDS             complement(3287..4189)
                     /locus_tag="PLC06_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRO89156.1"
                     /translation="MNRIQLTDNGPTFSRIVAGFMRLREWDMSTAALLDFVHQCLDLG
                     ITTFDHADIYGRYTCEELFGKALAAEPSLRGRIELVTKCGIQLVSPNRPNTRVKHYDT
                     SRKHIIASAERSLRNLHTDVLDLLLIHRPDPLMDADETAAALAELRSSGKIRHAGVSN
                     FTPRQFELLQSRLDFPLVTNQIELSVLYLEPLHDGTLDQLQQLRVPPMAWSPVAGGRL
                     FDPANERGRRVRTALESIGDALGASADQVALAWLLRHPARVLPVMGTGKIERLREAAA
                     AESIMLDRQQWFMIWEASAGREVP"
     gene            complement(4186..4542)
                     /locus_tag="PLC06_01545"
     CDS             complement(4186..4542)
                     /locus_tag="PLC06_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRO89157.1"
                     /translation="MELKTVTSWLLAKKGATEETPFGPDALVYKVMGKMFALVAWEEE
                     PLAISLKCEPGQALFLRDLYPAVRPGYHMNKKHWNTVTLDGTIPEDEVRGMIDDSYEL
                     VVKSLTRAMKEELDAL"
     gene            complement(4696..5850)
                     /locus_tag="PLC06_01550"
     CDS             complement(4696..5850)
                     /locus_tag="PLC06_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRO89158.1"
                     /translation="MSINTFQINDNHRLNLPTIDTPPRILLGAGPSNSHPRVFAAMIA
                     PTVGILDPTLIRVMDEISELLRYTWQTDNEFTFVTSGTGSSAMEAAFANVVEPGDRVL
                     VGVAGYFGRRMVEMAGIYGGDVRCLNAVWGEAFTLDEIRQGLEAHRPQVLALVHAETS
                     TGVRQPLEGIAELCREHDCLLLVDTVTSLGGVPLYADAWGLDISYSCSQKCLSTPSGV
                     APLTFGPRAMDKIRNRRSPIANWYMNANLLGKYWAGSPRVYHHTAPAHLFYALREGLR
                     LVAEEGLGERWARHQFVAETLWEGLDAIGLELHVAREIRLSSLTTVRVPDGVDAAAVS
                     RKLLTEYNIEIGGGLGDLAGKVWRVGLLGYNARRENVAVLLKALAEVLGR"
     gene            5900..6457
                     /locus_tag="PLC06_01555"
     CDS             5900..6457
                     /locus_tag="PLC06_01555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRO89159.1"
                     /translation="MIAPNLLRGENVRLTAVTSNDMPVITRWWSDPDFLRLYNTAPAV
                     PLNEDQLSRRFETSHTSPDNFLFAVRPLEDDELIGLLEFDGVVWAHRTTFVSIGIGEA
                     RHRGRGYGREAMQVGLNFVFNELNLHRVCLTVFSYNTAAIALYEALGFVREGVYREHL
                     ERDGRRHDMILYGLLRPEWLGNNPP"
     gene            6525..8069
                     /locus_tag="PLC06_01560"
     CDS             6525..8069
                     /locus_tag="PLC06_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRO89160.1"
                     /translation="MLNLAVLLEDSAREAPAKTAVIFNDMKLSYAAVNGAANQVANGL
                     VSIGIRPGDKVALTCPNLPHFPVAYYGILKAGAVVVPLNVLLKGREVAYHLADSDARA
                     YLCFQGTPDLPMGQFGYEGFQQTPGCEHFFMLTVDYTGPSPIEGTKTIGQLMAGQPPT
                     FDTVATHADDTAVILYTSGTTGSPKGAELTHSNMLLNARLSDTMYHAEPDDVHLVTLP
                     LFHSFGQTVQMNTGFYNKSALTLLPRFDPDTALSIMQRDKVTIFAGVPTMYWAMLNHP
                     NPEKFDLAQIAHNLRLAVSGGSAMPVEVMRAFGERFGVQILEGYGLSETSPVATFNRL
                     DHAPKPGSVGLPVWGVQVRVVDPLDHDVAQGEMGEIVMKGHNIMKGYYKRPEATADAM
                     RGGWFHSGDLGHKDEDGYVYITDRVKDMIIRGGFNVYPREIEEVMMTHPAISLAAVVG
                     VPDEGHGEEIKAYVILKQGQSAGEDELIAWCRDCMAGYKYPRIIEIRETLPMTATGKI
                     LKRELR"
     gene            8257..8346
                     /gene="kdpF"
                     /locus_tag="PLC06_01565"
     CDS             8257..8346
                     /gene="kdpF"
                     /locus_tag="PLC06_01565"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HRO89161.1"
                     /translation="MDFGLLLAGLIALGLLIYLIYSLLYPERF"
     gene            8348..10039
                     /gene="kdpA"
                     /locus_tag="PLC06_01570"
     CDS             8348..10039
                     /gene="kdpA"
                     /locus_tag="PLC06_01570"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRO89162.1"
                     /translation="MTFLELFQVIVFVGLLIVCTPFLGDYMKRVFDGERTIMSPVLRP
                     IERITYRLAGVNPEEEQTWKQYTLALLVFNVLGFLVLFLMQLLQGVLPLNPEHLPGVE
                     PLLAFNTATSFMTNTNWQSYAGEVTLSQLTQMLGLGVQNFLSPATGVAVVLALTRGIA
                     RKSAATLGNFWVDMVRSVLYVLLPLSIILAIILVSQGVVQNFDANVTATTLMGETQVL
                     PQGPAASQVAIKQLGSNGGGFFNTNSAHPYENPTPLSNFLEMLALLLIPAALTNTYGR
                     MVGSTRQGWAIFAAMMIMMVGLLSVMLVAEYATNPVLGLAESMEGKETRFGIAGTILW
                     VSATTSASNGAVNTMHSSLSPLAGGIAMLNMMLGEVIFGGLGAGLYGMLVFVILTVFI
                     AGLMVGRTPEYLGKKIEAREIKMATLAILFPAASILLFTALALATETGLSSRLSAGAH
                     GFSEILYAFVSTTANNGSAFAGLNANTPFYNITLGLALWIGRFGIIVPVMVIAGSMVG
                     KTAAPPSPGTFPTDQPLFVAMLIAVVLIVGALTFFPALSLGPIVEHFLLLSGRGF"
     gene            10058..12172
                     /gene="kdpB"
                     /locus_tag="PLC06_01575"
     CDS             10058..12172
                     /gene="kdpB"
                     /locus_tag="PLC06_01575"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRO89163.1"
                     /translation="MIHQSNPQPLFDPPIVGRAIVDAFRKLDPRQQARNPVIFVVAVG
                     AMMTSVILIADLLRGITDGLLFNLQITLWLWFTVLFSNFAEAMAEGRGKAQADSLRKT
                     RREMTARLLVGGNGSQKEKRVPAPELRIGDLVVCEAGDQIPGDGEVVEGIASVDESAI
                     TGESAPVIRESGGDRSAVTGGTRVLSDRIVIRITAEQGSTFLDRMIALVEGAKRKKTP
                     NEIALTILLSGLTIIFLLAVISLKPFADYAEAESGLTRTAVTIPVLIALLVCLIPTTI
                     GGLLSAIGISGMDRLIQRNVLATSGRAVEAAGDVDVLLLDKTGTITLGNRMATAFHPA
                     AGVDQARLAHAAQLSSLADETPEGRSVVILAKEKYGLRGQELAAPHAHFVPFTAQTRM
                     SGIDFPALNGRPAIAIRKGAADAVRQHVTVLGGRYPNDVDALVNHIAAEGGTPLVVAE
                     DREVLGVIELKDVVKGGMSERFAQLRKMGIRTVMITGDNPLTAAAIAAEAGVDDFMAQ
                     ATPEDKLRRIRQEQANGHLVAMTGDGTNDAPALAQSDVGVAMNTGTQAAREAGNMIDL
                     DSDPTKLIEIVEIGKQLLMTRGALTTFSIANDVAKYFAIIPALFGGIYAATGTTAGPL
                     SALNIMRLTSPESAILSAIIFNALIIVALIPLALRGIAYRPLGAAAVLRRNLLIYGLG
                     GLVIPFIGIKLIDIVITILGLV"
     gene            12192..12527
                     /locus_tag="PLC06_01580"
     CDS             12192..12527
                     /locus_tag="PLC06_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89164.1"
                     /translation="MSTRKKPAWRALLIIGLGSACALYVESQLPVDSQAHTWLLLAWV
                     ALFYGALTIWTKRNSEALDNEPPALDCTGRPIIDTNDQPAAKVQPKSRAYKPYTEPLY
                     FGSSRSRVN"
     gene            12529..13095
                     /gene="kdpC"
                     /locus_tag="PLC06_01585"
     CDS             12529..13095
                     /gene="kdpC"
                     /locus_tag="PLC06_01585"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HRO89165.1"
                     /translation="MFKQLRSALLMLVILTFVTGVIYPLIITGVAHLEFPGQARGSLV
                     GDPEQPVGSTLIGQANTDPRYFWPRPSATDYGTLPSGASNLAPTSLALAESVAIRAAD
                     FRAANGLGAADPVPVEMLFASGSGLDPHISPDAARLQVSRVAEARGLDVTRLQALVDA
                     HVEGPQLGFLGNPRVNVLVLNLALDALK"
     gene            13102..15798
                     /locus_tag="PLC06_01590"
     CDS             13102..15798
                     /locus_tag="PLC06_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HRO89166.1"
                     /translation="MFDEQRPNPDELLASIQKQEAQAERGKLKIFLGMAAGVGKTYAM
                     LLEARQLKAEGIDVVIGYIETHSRRDTELLLGGLELIPRQQVEYRGAWLEEMDLEAVL
                     ERRPALVLVDELAHTNAPGSRHAKRHQDVLDILEAGSDVYTTVNVQHFESRADAVREI
                     TGITIHETVPDTLLELANDIELIDLTPEELRKRLFEGKVYTPERVNVAADNFFRVGNL
                     TALRMMALRLTAERVDHQLQDYMELKRIAGPWKSGERLMVAIGPGPFSARLIRWTRRM
                     AYNLEAPWLAVFVESSHPLSDEARATVNRHFDLARSLGAEVLTVAGDDVPDTLLHLAR
                     RRNITQIIIGKPLTKRRIKLFNRGSYVERLIHDSGDIDVYVVTGDEPSNESSKAALPT
                     LPRIDRHSGWRGYLLAAGAIGLITLADLLIVPIAGYQLVGLTDLLAVLLVAIYLGRGP
                     ALLAAVLSALSWNYLFIEPHLTFQISLPQDIALLLLYFVIALFAGNLTARLRIREQQA
                     QYNAERNLALFTLTHETAGAVDMDAVLTTAVEQVGRVFDAEVAVLLPRVGGDGIVAHP
                     SGSLTMDEKEEGVANWVFEHGRVAGRNTDTLPKASARYYPLHTPSGIVGVIGIRRHAT
                     ERLPFDQESLLETFVGQIALAVEREMLDEAAEQAALLRESERLSAALLNSISHELRTP
                     IASIRGGADSLLQPGTGLGDDARRQLTHDILDASERLNRLVENLLDMSRLESGRLHLK
                     QEWCDVREIIGVAANRLSSCLMERSLTIDSQSDLPLVKLDFVLIEQVLVNLLDNACAY
                     TPPGTHIRIQARELNGWCELTISNDGPPIPTADLERIFDKFYRVPGQVITGTGLGLSI
                     SRGLVEAHGGTLTAENQPTGGVRFTLRLPMPDEPPTVREATQ"
     gene            15795..>16474
                     /locus_tag="PLC06_01595"
     CDS             15795..>16474
                     /locus_tag="PLC06_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO89167.1"
                     /translation="MSSGARVLVIDDEVQIRRFLRISLEANGYEVHEAAAGLDGISRA
                     AQLRPDLIILDMGLPDIDGLEVLRRLREWTTTPTIILSVRDADDDKVAALDAGADDYL
                     TKPFSLEELMARMRVTQRHALPGSQSHLFKAGDIAVDLSRRLVTRNGEAVKLTPTEYA
                     LLRLLIQHAGRVLTHKQILREVWGPEYVEETHYLRVYFAQLRQKLEENPSLPALILTE
                     PGVGYRLAA"
     assembly_gap    16475..16574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16575..16842)
                     /locus_tag="PLC06_01600"
     CDS             complement(<16575..16842)
                     /locus_tag="PLC06_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO89168.1"
                     /translation="MTVSMLEGLIVRIPEIHENRPIIGGTGVAVRTVVGHYKLGLSAE
                     EIADEMALDLAGVYAALAYFHLNRDEIEADLLANSEESVRHEIER"
     gene            complement(16935..18317)
                     /locus_tag="PLC06_01605"
     CDS             complement(16935..18317)
                     /locus_tag="PLC06_01605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRO89169.1"
                     /translation="MIQPGQQIENYRIDGVIGEGGMGTIYRATDVNLMRPVAVKVMHG
                     ELAEDPAFQERFLQEARAAARLDHPSIVRIYHFGRESGLLYIVMELIDGLSLGAYLRQ
                     LARLNQVVRLEETLSLVAQAADALGYAHRQGVIHRDVKPDNILVKRLDRADRPGDPPL
                     RAMVTDFGLAKLLGIEQDTQSGLLMGTLPYMSPEQVLDRPLDGRSDIYSLGVVLYQLA
                     TGKLPLDIRSPEGAVQAHQYEEIVAPREMHVGVPPAVETIILRALNRQPENRYQTAEE
                     LAADLRRTAATLGNQEARTFAEDSGSTIVSMVTEFPTPARRLEWDMRPRELRGEGVCR
                     VLLQNNAPTLQTISLTVETPRGGLYVDAAQKQVSLVPNQQGVVDFYLQPMKQPFIGRK
                     REWPFVVRVTPLSSPGSATEMDGQVVMAPQLPWWLALLLLVLIFALCGLGMWLLLSLP
                     VIGNLLSSLQ"
     gene            18434..18646
                     /locus_tag="PLC06_01610"
     CDS             18434..18646
                     /locus_tag="PLC06_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89170.1"
                     /translation="MPQLHLYIPDELAERIQREAQAADMSISRYLAQLVQREISPDWP
                     TGYFEDVVGGWKGGLLERPSQGTLEQ"
     gene            18675..19079
                     /locus_tag="PLC06_01615"
     CDS             18675..19079
                     /locus_tag="PLC06_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011377919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO89171.1"
                     /translation="MYLLDTNACIRILNGQSISVVERLRRQSPRAVYLCSIVKAELIY
                     GAHRSARPAENMRLLNRFFEPYESLPFDDRCTEVYGRIRNELERSEMVIGPNDLMIAA
                     TAVTHRLVLVTANTRQFSRVINLEIENWELDK"
     gene            complement(19100..19597)
                     /locus_tag="PLC06_01620"
     CDS             complement(19100..19597)
                     /locus_tag="PLC06_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89172.1"
                     /translation="MIISVTPTTLIQPSATPCQIPVQAGLADAWDIELLGCPINPGAA
                     AINTAYAPFEGGQMLWRGDTDLIYVLTNDGRWTSFPNMWREGDPPFTCDKEDPAITPI
                     RGFGRVWCDNPGIRGALGNVTAAEIGDSASAAQDFVNGTILAAPFGDLFIFVGEDGVW
                     RRVDH"
     gene            complement(19771..20316)
                     /locus_tag="PLC06_01625"
     CDS             complement(19771..20316)
                     /locus_tag="PLC06_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRO89173.1"
                     /translation="MRLFGWRAVGEIPDLPRFVLVGAPHTSNLDFFMTALTMAALGVD
                     VHFVMKHTPFVGPVGWFLRWFGGIALDRDRSRDFVSQMVDEFEHRDQFLLAIMPEGTR
                     GKDRDSATKGWRSGFYYIAKGAGVPLVMVLFDYANKRMRVGPMVWPGDSYEADLPRIQ
                     ALFAGIEGRNPERMMALEKGA"
     assembly_gap    20517..20616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20617..22347
                     /locus_tag="PLC06_01630"
     CDS             <20617..22347
                     /locus_tag="PLC06_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRO89174.1"
                     /translation="RFRNGDPTNDPTAASLEGAWPHDHTSPWEVHPWGSDWYAAQPYE
                     QQNGRDIWYNIQRRRYGGDLQGVIDSLDYLVDLGIEAIYLNPVFDSPSLHKYDGATYH
                     HIDPHFGPDPDGDRRLIAIENPIDPATWQWTSADRLALRLIDEAHRRGLRIIFDGVWN
                     HMGINSFAFRDVVARQRDSVYRDWFRIDSWDDPAAGTTFAYTGWFGAHELPEILQDEN
                     GHAPGPRDYIYAATRRWMDPDGGGDPSDGIDGWRLDVAFCIRHPFWKGWRQHVRSINP
                     EAYLVAEVVQSPDEEKPYLEGDEFDATMNYNFFIDLYEFFISRDYRVTATEFDARLRY
                     LREAHPAQVAHVMQNLLDCHDTSRIASHIVNRDIWNIREWNTFFQRTKPADTPEFDTR
                     RPTAAERRIQRLVALFQMTYIGAPMIYYGDEAGMWGGNDPCCRKPMVWPDMVYNAEAV
                     LPDGTLRPTADEVAFDHELHDYYRQIIALRKASPALRHGEYRTLLTDDARHLFAFARE
                     TEDDAAVCVFNADDLHHIVELPVGDGQWREGFTPQESSETSEVLDGVIHVAIPPHSGI
                     IFRRTVEPVS"
     gene            complement(22539..24317)
                     /locus_tag="PLC06_01635"
     CDS             complement(22539..24317)
                     /locus_tag="PLC06_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRO89175.1"
                     /translation="MGPIAHAKGTAFRVWAPNAQAVYVIGDFNDWKENANPLVHEVDG
                     YWSIDVAGAKPGQEYRYIIVNGEQRLHRLDPYSRQVTSSVGNSLIHDPNFDWGGAGNF
                     QSASWNELIIYEMHIGTFNPMSEERVGTFQRAARKLSYLRTLGVNAVQIMPPMEFPGG
                     RSWGYNPALPFAVETDYGGMMGFKQFVKTAHGHGIAVILDVVYNHLGPGDLDLWRFDG
                     WSEGDGGGIYFYNDRRNETPWGHTRPDYGRPEVRQMLRDNALMWLHEYKVDGLRWDAT
                     AYIRNIFGNDNEPANDLPDGWSLMQWINEEVHEQEPNNLTIAEDLRNNEWLVKDTGAG
                     GAGFGSQWDANFVHPVRTAIISSDDAFRDMKAVKKALTFKYAGDVFKRVVYTESHDEV
                     ANGKARVPEEIWPGNSHNWYAKKRSTLGAVLVFTAPGIPMIFQGQEFLEDKWFTDTVP
                     LDWEYVKEEQGIVYLYRDLMHLRRNLKGTTRGLTGQHIKVYHMNQKDKVLAYHRWYDG
                     GPGDSTVVVINMANRTLTDYVIGFPAAGKWSLRFDSHAAGYSPDYKGQVSVDVVAEEG
                     AYDGLPAHGKVNVAPYSGVIYSQNPG"
     gene            24742..25818
                     /locus_tag="PLC06_01640"
     CDS             24742..25818
                     /locus_tag="PLC06_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89176.1"
                     /translation="MRVAVLANLIKNAPTWPGMPEDRWDDLDSEETIAAIVAALETGG
                     HDVTFLEGDLSLFETLPKLKPDICFNICEGHWGDSREAQVPAILEMLRVPYTGSGPLT
                     LALALDKPMTKRVLWYHSLPTPTFQTFERVHEPLDPDMTFPLFVKPSREGTGMGVSAD
                     SIVHNEEQLRAQLRYQFEKFDQTILVEHFIEGREVTVGLVGNLSTPVARRLPDDLEAA
                     RISRGLHFFPPLEVDMSRYPVEEAGVYTSRIKTELVHTFHYLCPAPLPEATIEELNWL
                     AAATFRVTGSFDVGRIDFRLDANDNNKPYILEINPLPGLNPGYSDLPLEAKAAGWTYE
                     QLINRILEEAVDRYGLEEDASRPA"
     gene            25846..26103
                     /locus_tag="PLC06_01645"
     CDS             25846..26103
                     /locus_tag="PLC06_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89177.1"
                     /translation="MPAKDPSLMARCLECDSRIYFERKPDPGEIIVCPECETSLEVIR
                     SSPIKFGWPDDAGQGGSSIPRVDLDEFFNELADNESEDEDY"
BASE COUNT         5320 a         8154 c         7274 g         5172 t
ORIGIN      
        1 cctgcgcggg caacacatcg tgcccctgcc cgcaagcctg acgagtggtc gataccggtt
       61 cttcctcggc gatttcccct tggaagaaat taccgtcatc gcgccggagc gcagcttcac
      121 ccagccggag atcagccaat cgatcgatgc cgcgttcgcc acggccgacg gcgcgccggt
      181 cgccactctg gtgggcctcg ccgtcgattc gtcctcgtcc tcttgctccc ctgcgcccct
      241 gctcccctgc gcccttcctc tcgtctggcg cgccgacggc gaagcgagcg ccggctatca
      301 tgccttcgtc catctggtgg acgaggcggg caacatcctg gcccagtcgg acgcggttcc
      361 ggcgggctgg acgcggccga ctaccggctg gctgcccggc gagtacatca tggatgtcca
      421 cacgctgaca ctgccgcctg agttgccgcc cggccaactc gccttccgcg caggtctcta
      481 cgatcccgtc acggacgagc gcttgtccgt caatggtacg gatgccgtac tcattacctt
      541 gccgggatcg tgagccttta tatcgccgtt atttgcatcg atatgcattt gacggccccc
      601 agaccaaggc gggcgagagt atttgcacat ccgcatatgt atagtagtca ctccagcttg
      661 tcgagatgat gtagttgcac tgagcgccgg agcacgtttt actattggcg gtttgcccgc
      721 cgttccacct caatgtgtag tatgaggttc ctgtcgttga ggaataatca aattttgctc
      781 ctgcggagca agtcgcgtac agatcgtcgg caaagattga ccaggatagt gggttgggat
      841 tacccttctt atccagtgtg ggcgttttgc tttgcggctc atcaatgaca agatcgatca
      901 agcctgtgtc gttgttaaat tcaatcctct cgatttgcag aaggtcatca gtattgaatg
      961 gcacatcctt ctgagcatct tgagtatcag ttggcagagg atcaatcacc tcgatctgtt
     1021 cggtaatagt cccttcagga acctcaataa cattcgtata aactttgaca ggcttccctt
     1081 ccagattggg aaagggtgaa tcaacttctt ccatcgactc agtgacaatt ccttccggga
     1141 tttctcttgt ctcaataaca accgggggat atggattatc ggccgaagtt gagcgcgtaa
     1201 atacgatcaa accagctaat agaatggcgc tcacgccaac caacaaaagc gctttgaatc
     1261 tgaacatggc agtatctcct tatccttaaa tgaattgtag tgggttcact actgccatta
     1321 ggttaacaaa ccgcatgtaa agcaggtgtt aagtttttgg gagacgcaca atgaaggtgc
     1381 tgccgactcc gactgtgctt tctatatcaa ggcggcccct atataagtga acaaggtgcg
     1441 cgacgatgga taagcccagc ccggttcccg ggtcatgacc agaggagggg acagggcggc
     1501 ggtagagtgg ctcaagaatc aggctctgct cctcagccgg aatgcccaca cctgtatcag
     1561 ccaccgagat aactatccat gccggctcct cccaggccga cacttcaacc gatccacccg
     1621 gtcgattgta gcgaattccg ttctcgatca agttgctgaa aatcgagctt atcaagacat
     1681 catccccgtt cagaaatgac tctgtttggg caactagacg caagctgacc tgatattttt
     1741 ctgcctgcgg agacaaattg gcgaccactt cttccagcaa aggcagaagc gatatttcct
     1801 tatcgactga ttcatactct ccccttgtca gcaaaagcaa attgcttacc aatcgttcca
     1861 atcgcgcaag catttgttcc tgggttattg ccatttcctg atagtcagct aacgtcgccc
     1921 gcggatcatc gaggattacc tccagattcg ctcgcagggc agccaaggga gtacgtaatt
     1981 cgtgggcggc attagaggcg aagcgtctct gctgcccgaa tgaatagtcg agccgctcaa
     2041 gcgcattgtc aaaagcatct gccaatcggc taacctcgtc cacagcacct tcaacagata
     2101 accgcttgcc aaggttgccg ctatcgattt cggcaatctc ctgactgaga taggaaatag
     2161 gccgaagggc gcggcccgcc aaaaaataag cgccggcact acccaaaatg atcataaagc
     2221 ccataaccac cagcgagata gcgcgtactc cttgtaatgt cagatcgatg ataagcgagg
     2281 aattccggcg cggaatttgg atggatggta gagaagagga atttggagca accgagggat
     2341 catcactctc ttccgaggca aaatcctgga gaggaatgtc gctcaccgtt tgaggaatca
     2401 ggattgccgc cactacattg acagccagaa caaagccaat actcgtaata gcaagtaaca
     2461 cgataaacca aagcgtcagg cgcaaccgca aagttagaac caggcgtaac cgctggtgta
     2521 atttgttcat gcgaccatag ctccctctcg cactgtatgg ctctcatgtg agcaagcgat
     2581 agccctctcc cgcaactgtc tcgatataac gcggggtctg aggatcatcg ccgagtttgc
     2641 gtcgaagaga attgatatgg acgcgaaccg tatgtgtaaa tgggtttaca gatgagtccc
     2701 atacgtgttc caacagttct tcctgactta tgacttcacc tggccgggac atcaaatagg
     2761 ccaggatacc gtattccttg cgggttaatt ccagacggcg atcctcttgc caaactattt
     2821 gtgctgccgg atccagcttg agatcactca cggatcgaat cggctctcgc acgcgcaaat
     2881 cacggcgcaa cagcgcacga atacgagccg acaactcctc aaaatggaag ggcttcacaa
     2941 tataatcatc cgctccactg tcaagcccgg ctattttgtc cgataagcgg tcccgcgccg
     3001 ttaaaataag gattagcaac tcaggctttt cggctcgcaa acggctgcat atttcaagac
     3061 cgtctgctcc gggaagattg aggtcaagga taagcaaatc gtattcattc acccccgtga
     3121 attcctggct ctcttcgtca ttagctgcaa tatcaactgc aaacccttgc cggcgcaacc
     3181 ctttagcgat cgcctgggcc aatacaactt catcctcgat aattaacagc cgcattacat
     3241 tcgtacccac ttcttcagcg ctcataggcg cactcacgcc aaggctctac ggcacttccc
     3301 tccccgccga agcctcccaa atcataaacc actgttgccg gtcaagcatg atgctctccg
     3361 ccgcggccgc ctcgcgcagc cgctcgatct tgcccgtgcc catgaccggc agcacccgtg
     3421 ccggatggcg cagcagccag gccagggcta cctgatccgc cgaggctccc agcgcgtccc
     3481 caatcgattc cagagccgtc cgcacccgcc gccctcgctc gttggcgggg tcgaacaggc
     3541 ggccgccggc caccggcgac caggccatcg gcggcacgcg caattgttgc aactggtcga
     3601 gcgtgccgtc atggagcggc tccagataca gcaccgacag ctcgatctgg ttcgtcacca
     3661 gcgggaagtc gagccgcgat tgaagtagct caaactggcg cggggtgaag ttgctgacgc
     3721 cggcgtggcg tatcttgccg gagcttcgca actcggccaa cgctgcggcc gtctcatcag
     3781 cgtccatcag cggatcgggc cggtggataa gcagcaggtc cagcacgtcg gtgtgcaggt
     3841 tgcgcagcga acgctcggcc gaagcgatga tatgcttgcg gctggtgtca taatgtttga
     3901 cgcgcgtatt cggccgattg ggcgacacaa gctggatgcc gcacttggtc accagttcga
     3961 tgcggccgcg caaggacggc tcggcggcca acgctttgcc gaacaactcc tcgcaagtgt
     4021 agcggccgta gatgtcggcg tggtcgaagg tggtgatgcc cagatcgagg cactggtgga
     4081 cgaaatcgag caaggcggcc gtgctcatat cccactcgcg caggcgcatg aagccggcga
     4141 cgatgcgcga gaaggtcggc ccgttatcgg tcagttgaat ccggttcata gggcgtccag
     4201 ctcttccttc atggcgcgcg tcaggctctt gacaaccagc tcatacgagt cgtcgatcat
     4261 gccccttact tcgtcttcgg gaattgtgcc gtcgagcgtc actgtattcc aatgcttctt
     4321 gttcatgtga taacccggcc ggacggcggg gtacagatcg cgcaggaaaa gcgcctgccc
     4381 cggctcgcac ttcagcgaga tggcgagcgg ctcttcttcc caggcaacga gcgcgaacat
     4441 cttgcccatg accttgtaga ccagcgcgtc cggcccgaaa ggtgtttcct cggttgcgcc
     4501 ctttttggcg agcaaccagg atgtcacggt cttgagttcc ataatgaacc gattttcagt
     4561 gaatcagtag gtcagtattc agtgggtcag tattcagtgc atcggcgtcg atctatttca
     4621 ggtcaggttg atcgactgac cactgcccca ctgatctact gacctactga cctactgacc
     4681 tactgttcta ccgtcctatc gccccaacac ttccgccagc gccttcagca ggacagccac
     4741 attctcgcgg cgagcgttat agcccaggag gcctacgcgc cagaccttgc cggccaggtc
     4801 acccaagcca ccgccgatct cgatgttgta ctccgtcaac agcttgcgcg acacggccgc
     4861 ggcgtcaacc ccatcgggca cacgcaccgt ggtcagactc gatagccgga tttcgcgcgc
     4921 gacatggagt tccagaccaa tcgcatccaa cccttcccac aacgtctcgg ccacaaactg
     4981 gtggcgcgcc caacgctcgc ccagcccttc ctcggccacg aggcgcaacc cctcgcgcaa
     5041 ggcataaaac aaatgcgccg gcgcggtatg gtgatagaca cgcggcgatc cggcccagta
     5101 tttgccgagc aggttggcgt tcatatacca gttagcgatc ggcgagcggc ggttgcgaat
     5161 cttgtccatc gcccgcgggc cgaacgtgag cggcgcgacg cccgacgggg tgctcaggca
     5221 tttctgcgag cagctatagg agatgtcgag gccccaggcg tcggcataga gcggcacgcc
     5281 gcccaggctg gtgacagtat caaccagaag caggcagtcg tgctcgcgac aaagctcggc
     5341 gatcccttcg agcggctggc ggacgccggt tgaggtttcg gcatggacaa gggccagcac
     5401 ttgcggccgg tgggcttcca ggccttgccg aatctcatcc agggtgaagg cttcacccca
     5461 cacggcattg aggcaacgaa catcaccgcc gtaaatgccg gccatttcaa ccatgcggcg
     5521 gccgaaatag ccggccacgc cgaccagcac ccggtcgccc ggctcgacaa cgttggcaaa
     5581 cgccgcctcc atggccgaac tgcctgtgcc gctggtgacg aaagtgaact cattgtcagt
     5641 ctgccacgta tagcgcagca gttcgctgat ttcgtccatc accctgatta atgtcgggtc
     5701 gagaatacca acagtcggcg cgatcatggc cgcaaagacg cgcggatggc tgttcgacgg
     5761 ccccgcgccc agaagaattc gcggcggtgt gtcgatggtc ggcagattca aacggtgatt
     5821 gtcattgatt tggaaggtgt ttatactcat gtctgtcctc gccccatacg gcgaatcagc
     5881 gccacacggg agaactgtca tgatagcacc taacctgttg cgcggcgaaa acgttcgact
     5941 gacggccgtc acatccaacg acatgccggt catcacgcgc tggtggagcg accccgactt
     6001 cctgcgcctc tataacacag ccccggccgt cccgctcaac gaagatcagc taagccgccg
     6061 cttcgaaaca agccatacca gccccgataa ttttctcttc gccgttcggc cattggaaga
     6121 cgatgaactc atcggcctgc tggaatttga cggcgtcgtg tgggcacacc ggacgacgtt
     6181 tgtcagcatc ggcatcggcg aagcgcgcca ccgcggccgc ggctatggcc gcgaggccat
     6241 gcaggtcggt cttaacttcg tcttcaacga gttgaatctc caccgcgttt gcctgaccgt
     6301 gttcagctat aacacagcgg ccattgcttt atatgaggcg ctgggctttg tgcgcgaagg
     6361 cgtatatcgc gagcatctcg aacgggacgg ccgacgccac gacatgattc tctatggcct
     6421 tctgcggcct gagtggctgg ggaacaaccc tccatagtca gaccccatca cgcaaacaaa
     6481 ctccataaca ttcaccaatc ctcattcaat ttaggagaca atcaatgcta aacctcgcag
     6541 tacttttgga agacagcgcg cgcgaagcgc ccgcaaaaac ggccgtcatt ttcaacgata
     6601 tgaagctcag ctacgccgcc gtcaacgggg ctgccaatca ggtcgccaac ggattggtga
     6661 gcatcggcat ccggccgggc gacaaggtag ccctgacctg ccccaacctg ccgcactttc
     6721 ccgtggccta ttacgggatt ctgaaggccg gggcggtcgt cgtaccgctc aacgtcctgc
     6781 tcaaagggcg cgaggtcgcc tatcatctgg ccgactccga tgcccgggct tatctttgct
     6841 tccagggcac gcccgacctg ccgatgggac aattcggcta cgaaggcttc cagcagactc
     6901 ccggctgcga gcatttcttt atgctcaccg tcgattatac cgggccgtcg cccatcgagg
     6961 ggacgaagac gattggacaa ctgatggccg ggcagccgcc gacgttcgac acggtcgcca
     7021 cccatgccga cgacacggcc gtcatcctct acaccagcgg caccaccggc agccccaagg
     7081 gcgcggaact cacccacagc aacatgttgc tcaatgcccg tctgtcggac acgatgtacc
     7141 atgccgaacc ggacgatgtg catctggtaa ccttgccgct gttccactcc ttcggccaga
     7201 cggtgcagat gaacaccggc ttctataaca agtccgccct gacgctattg ccgcgcttcg
     7261 accccgacac ggccctgagc atcatgcagc gcgacaaggt aaccatcttc gccggtgtgc
     7321 ccacgatgta ttgggccatg ctcaaccatc ccaacccgga gaaattcgat ctggcccaaa
     7381 tcgcccacaa cctgcggttg gccgtatccg gcggctcggc catgccggtc gaggtcatgc
     7441 gcgccttcgg cgagaggttc ggcgtgcaga ttctggaagg atacggcctc tccgagacca
     7501 gccccgtggc aacgttcaac cgcctcgatc acgcgcccaa acccggctcg gttggtctgc
     7561 cggtctgggg cgtgcaggtg cgcgtggttg acccgctgga tcacgacgtg gcccaaggcg
     7621 agatgggcga gatcgtcatg aaaggccaca acatcatgaa aggctattac aaaaggccgg
     7681 aagccacggc cgacgcgatg cgcggcggct ggttccattc cggtgacctt gggcataagg
     7741 atgaggacgg ctatgtctac atcaccgacc gcgtgaaaga catgatcatc cgcggcggct
     7801 tcaacgtcta cccgcgtgag atcgaggaag tgatgatgac ccatccggcg atcagcttgg
     7861 cggccgtcgt cggcgtgccc gacgaaggac atggcgagga gatcaaggcc tacgtcatcc
     7921 tgaaacaggg ccaatctgcc ggcgaagatg aactgatcgc ctggtgccgt gattgcatgg
     7981 ccggctacaa atatccgcgc atcatcgaga ttcgcgagac gctgccgatg acggcaacgg
     8041 ggaagatcct caagcgagaa ctgcgataaa ccctgtagcg cagtcattcc cgactgcact
     8101 atgtccaatc agaggcgcaa agatagcatt ctttgcgcct cttcttttat tccatccaat
     8161 cttgatattt tcttgatgtc tttgcgccgt ttcttgagcg cggcgcgtga tgtcctggcc
     8221 gataataccg atgaaccact tacaaggaga atcggtatgg atttcggctt gttattggcc
     8281 gggctgatcg ccctgggcct cctcatttat ctcatctact cattgctcta tccggagaga
     8341 ttctgatatg acgttccttg agctattcca ggtgatcgtc tttgtcggcc tgctcatcgt
     8401 ttgcacgccg tttctgggcg actatatgaa gcgtgtcttc gacggcgagc ggaccattat
     8461 gtcacccgtg ctgcggccaa tcgagcggat cacctatcgg ctggcaggcg tgaaccccga
     8521 agaagaacag acctggaaac aatatacgct ggccctgctg gtctttaacg tcctgggctt
     8581 cctggtcctg tttctcatgc aattgttgca aggcgtgttg cccctcaacc cggagcatct
     8641 gccgggtgtc gaaccattgc tggccttcaa tacggccacc agcttcatga ctaacaccaa
     8701 ttggcaatcc tatgccggcg aggtgacgct gagccaattg acgcagatgc tggggctagg
     8761 cgtgcagaac ttcctcagcc ccgcaacggg cgtcgccgtt gtccttgcct tgacgcgcgg
     8821 catcgcccgc aagtcggccg ctactctggg caacttctgg gtcgatatgg tgcgctcggt
     8881 actctatgtc ttgctgccgc tctccatcat cctggccatt attctcgtga gccagggagt
     8941 ggtgcaaaac tttgacgcca acgtgacggc cacgacattg atgggcgaaa cccaggtctt
     9001 gccgcaaggc ccggccgcgt cgcaagtcgc catcaagcag ctcggctcca acgggggcgg
     9061 cttcttcaac accaacagcg cccatcccta cgagaacccg acgccgctga gcaacttcct
     9121 ggagatgctg gcgcttctgc tcatccccgc cgcgctaacc aacacctatg gccggatggt
     9181 cggctccacc cggcaaggat gggccatctt tgccgccatg atgatcatga tggtcggcct
     9241 gctctcggtc atgctggtgg ccgaatacgc gacgaacccg gttctgggtt tggccgagtc
     9301 gatggaaggc aaggaaaccc gattcggcat cgccgggacc attctgtggg tttcggcaac
     9361 gacgtctgcg tcgaacggcg cggtcaacac gatgcacagc agcctaagcc cgctggccgg
     9421 cggcatcgcc atgctgaaca tgatgctcgg cgaggtgatt ttcgggggtc tgggtgcagg
     9481 cctctacgga atgctggtat tcgtcattct gactgtcttt atcgccggtc tgatggtcgg
     9541 ccgcaccccc gagtatctgg gcaaaaagat cgaagctcgc gagataaaga tggcaaccct
     9601 ggccatcctg tttcccgcgg cgtcgattct gctgttcacc gcactggctc tggcgaccga
     9661 gaccggcctg tcgagccggc tcagcgccgg agcccacggc ttcagcgaaa tactctatgc
     9721 cttcgtctca accaccgcca acaacggcag cgccttcgcc gggctaaacg ccaatacgcc
     9781 cttttataac atcaccctgg ggctggcatt atggatcggc cgcttcggaa tcatcgtgcc
     9841 cgtgatggtc atcgccggca gcatggtcgg caagacggcc gcgccgccgt caccgggcac
     9901 gttcccgacc gaccaacccc tgttcgttgc catgctaatc gccgtcgtac tgatcgtcgg
     9961 cgcgctgact ttcttcccgg cgctgtcgct aggcccaatc gttgaacact tccttctatt
    10021 atccgggcgg ggattttagt cacggagtaa caagtaaatg atccaccaat caaatcccca
    10081 acccttgttc gatcccccga ttgtggggcg cgcaatcgtt gatgcttttc gcaagctcga
    10141 cccgcgccaa caggcccgga acccggtcat ctttgtcgtt gccgtcggcg cgatgatgac
    10201 gtcggtcatt ctgatcgccg atctgctgcg cggcattacc gacgggctgc tgttcaacct
    10261 gcagatcacg ctctggctat ggttcacggt gttgttctcc aatttcgccg aggcgatggc
    10321 cgaggggcgc ggcaaggctc aggctgacag cctgcgcaag acacggcgcg aaatgaccgc
    10381 ccgcctgctg gtcggcggga atggctctca aaaggaaaaa cgcgtcccgg cgccggaact
    10441 gagaatcggc gacctggtag tttgcgaagc gggtgatcag atacccggcg acggcgaagt
    10501 cgtcgagggc atcgccagcg tcgacgaatc agccattacc ggtgaatccg ccccggtcat
    10561 ccgcgaaagc gggggcgacc gcagcgccgt caccggcggc acgcgcgtcc tcagcgaccg
    10621 gattgtcatt cgtatcacgg ccgagcaagg cagcaccttt ctggacagga tgatcgccct
    10681 ggtagagggc gccaagcgca aaaagacacc gaacgagatc gccctgacta tcttgctctc
    10741 cggcctgacg atcatcttcc tgttggccgt catctcgctg aaaccgttcg ccgactacgc
    10801 ggaagccgaa tcgggcctga cgcgcacggc ggtgacgatc ccggtgctca tcgccctgct
    10861 cgtctgtctc atcccgacga cgatcggcgg cctgctaagc gccatcggca tcagcggcat
    10921 ggatcgcctc atccagcgca acgtattggc cacgagcggc cgggccgtcg aggcggccgg
    10981 cgacgtggac gtcctgctat tggataaaac ggggacgatc acactcggca atcgcatggc
    11041 aaccgctttc catccggcag cgggtgtcga ccaggcgcgc ctggcccatg ccgcccaact
    11101 ctcctcgctg gctgatgaaa cacccgaagg ccgctccgtc gttatcctgg ccaaggaaaa
    11161 gtacggtctg cgcgggcagg aactggccgc gccgcacgcc catttcgtgc ccttcaccgc
    11221 ccaaacccgc atgagcggca tcgacttccc ggcgctgaac ggccggcccg ctatcgccat
    11281 tcgtaaaggc gcggccgacg ccgtgcggca acatgtcact gtgctcggcg gccgctatcc
    11341 aaacgatgtc gacgcgctcg tcaaccacat cgccgcggaa ggcggcacgc cgctggttgt
    11401 ggctgaggat cgtgaagtgc tcggcgtaat cgaactgaag gacgtggtca agggcggcat
    11461 gagcgaacgc ttcgctcaac tgcgcaagat gggcattcgc accgtcatga tcaccggcga
    11521 caaccctctg acggccgcgg ccattgccgc cgaagcgggc gtcgatgact tcatggccca
    11581 ggccacgccc gaagacaagc tgcggcgcat ccgccaggaa caggccaacg ggcatctggt
    11641 ggccatgacc ggcgacggca cgaacgacgc ccccgcgctg gcccaatccg acgtcggcgt
    11701 ggccatgaac accggcaccc aggccgcacg cgaagccggt aacatgatcg atctggacag
    11761 cgatccgacc aagctgatcg agatcgtcga gatcggcaag caactgctga tgacccgagg
    11821 cgcgctgact accttcagca tcgccaacga cgtcgccaaa tatttcgcca ttatcccggc
    11881 cttgtttggc ggaatctatg cggcgaccgg aacaacagcc ggcccgctca gcgctctgaa
    11941 catcatgcgc ctgacctcgc cggagagcgc catcctgagc gccatcatct tcaacgcgct
    12001 gatcattgtc gccctgatcc ctctggcgtt acgcggcatc gcttaccggc cacttggggc
    12061 ggcggccgtt ctgcggcgca atctgctcat ctatgggttg ggggggctgg tgattccgtt
    12121 catcggcatc aagctcatcg atattgtgat aacgatcctt ggattggtct aactaacagg
    12181 agtaaaagat catgtctact cgcaagaaac ctgcctggcg tgcgctatta atcattgggc
    12241 tgggtagcgc atgtgcgctc tatgtcgaat cacaactgcc tgttgattca caggctcata
    12301 cctggctgct gctggcctgg gtggccctgt tttacggcgc tttgactatc tggacaaagc
    12361 gtaatagcga ggcattggac aacgaaccac ctgcgcttga ttgcacgggg cggccaatca
    12421 tcgataccaa tgaccagccg gcggctaaag tccaaccaaa aagccgcgct tataaacctt
    12481 acacagaacc actttacttt ggctccagtc gttcaagggt aaactaacgt gttcaaacaa
    12541 cttcgatccg cattgttgat gctggtaata ttaaccttcg tcaccggggt catctatcca
    12601 ctgatcataa ccggtgtcgc ccatctggaa tttccgggcc aggctcgtgg cagccttgtg
    12661 ggcgatccgg agcaacccgt cggttcgacg ctgataggcc aggccaacac cgacccacgc
    12721 tacttctggc cgcggccatc ggctaccgac tatggcactt tgccttccgg ggcgagcaac
    12781 ctggccccga caagcctggc tctggctgaa tcggtggcga tacgggcggc cgactttcgc
    12841 gccgccaacg ggctgggcgc ggcagacccc gtgccggtcg agatgctctt cgcttccggc
    12901 agcggcctcg acccgcatat cagccccgac gcagctcgat tgcaagtcag tcgtgtcgcc
    12961 gaggcgcgcg gactggacgt aactcggcta caggcgttgg tcgacgccca tgtggaaggc
    13021 ccgcaattgg gctttctggg aaacccacgg gtcaatgtcc tggttctgaa tttggcgctg
    13081 gatgcattaa aatagctgca catgtttgac gaacaacgac ccaatcccga tgagttgctg
    13141 gcttccatcc aaaaacagga agcacaagcg gaacgcggca agctgaagat ctttctgggg
    13201 atggcggccg gcgtaggcaa gacctacgcc atgcttctgg aggcacggca gctaaaggcg
    13261 gaaggtatcg atgtcgtcat tggttacatt gagactcaca gccgacgcga caccgaactg
    13321 ctgttgggcg gcctggagtt gatcccacgc caacaggtcg agtatcgcgg cgcgtggctg
    13381 gaagagatgg acctcgaggc cgtgctggag cggcgccccg ctctcgtcct cgtcgacgaa
    13441 ctggcccaca ccaatgctcc cggttctcgg cacgccaaac ggcatcagga cgtactggac
    13501 atcctggagg cggggagcga cgtttacaca acggtcaacg tccagcattt tgagagtcgg
    13561 gccgacgccg tgcgcgagat caccggcatc accattcatg aaaccgtccc cgacacgctg
    13621 ctcgaactgg ccaacgacat cgagctgatc gatctgacgc ctgaagagct gcgcaagcgg
    13681 ctgtttgagg gcaaggtcta tacgcccgaa cgggtaaacg tcgccgcaga caacttcttc
    13741 cgcgtcggca acctgaccgc cctgcgtatg atggctttgc gcctgacggc cgagcgcgtc
    13801 gaccaccagc ttcaggatta catggagctg aagcgtatcg ccgggccgtg gaaatcaggc
    13861 gaacgactga tggtcgccat cggccccggc cctttctccg cccggctaat ccgctggacg
    13921 cggcgcatgg cttataatct ggaagcgccc tggctggccg tattcgtcga aagctcgcac
    13981 ccgctctccg acgaagcccg ggccacggtg aaccgccatt tcgatctggc gcgctcgctc
    14041 ggcgcggaag tgctcaccgt cgccggggat gacgtgccgg acacgttgct ccatcttgct
    14101 cgccggcgca acatcaccca gatcatcatc ggcaagccgc taaccaaacg gcgaatcaag
    14161 cttttcaacc gtggctccta tgtcgagcgg ctcatccacg acagcggcga tatcgatgtg
    14221 tacgtggtga ccggcgatga accgtcgaat gaatcaagca aggccgctct gccaacacta
    14281 ccgagaatcg atcgtcactc cggttggcgg ggatacctgc tggcagccgg cgcgattggg
    14341 ctaatcaccc tggccgatct attgatcgtg ccgatcgccg gctatcagct cgtggggctg
    14401 accgatctgc tggccgtact gctggtcgca atctatcttg gtcgtggccc ggccctgctg
    14461 gcggccgttc taagtgccct cagctggaac tatctgttca tcgaaccaca cctgacgttc
    14521 cagatttccc tgccacagga catcgctctg ctcttgctct acttcgtcat cgcgctcttc
    14581 gccggcaacc tgactgcccg attgcgcatt cgcgagcagc aagcccaata taacgccgag
    14641 cgcaatctgg cgctcttcac cctgacccat gagacagccg gcgcagtcga tatggatgct
    14701 gtcctgacca cggccgttga acaggttggc cgtgtttttg atgccgaagt cgccgttctg
    14761 ctgccacgcg tcggaggaga cggtatcgtc gcgcacccaa gcggctcgtt gacgatggat
    14821 gaaaaagaag agggagtggc caactgggta tttgaacacg gtcgggttgc cggccgcaac
    14881 accgacacgc tcccgaaagc ttcggctcgc tactacccgc tgcacacgcc aagcggaatc
    14941 gtcggcgtta tcggcattcg ccggcacgcg acggagcgat tgccgttcga tcaggaatca
    15001 ctgctggaga cattcgtcgg tcagattgcc ctggccgtcg agcgggagat gctggacgaa
    15061 gccgccgagc aggccgccct gctgcgggaa tcggagcgcc tgtcggccgc tttgctcaac
    15121 tccatctctc acgaactgcg cacaccgatc gcctcgatcc ggggcggggc cgatagtttg
    15181 ctccagcccg gcacagggtt gggagatgac gcccgccgcc agttgaccca cgacatcctc
    15241 gatgcttccg agcggctaaa ccgactggtg gagaatttgc tcgatatgtc ccggctggaa
    15301 agcggccggc tgcatctgaa gcaagaatgg tgtgatgtgc gcgagattat cggcgtggca
    15361 gccaatcgat tatcgagttg cttgatggag cgatcgctga ccattgactc gcagtccgat
    15421 ctgccgctgg tcaagctcga cttcgtgctc atcgaacaag tgctggtcaa tctgctcgac
    15481 aatgcttgcg cctacacgcc tcccggaaca cacattcgaa tccaggcacg cgagctaaac
    15541 ggatggtgtg aattgaccat ctcaaacgac ggcccgccta tcccgactgc cgatttggaa
    15601 cgtatcttcg acaagttcta tcgtgtcccg gggcaagtca tcaccggcac tggattgggt
    15661 ctgtctatct cgcgcgggct ggtagaagcc catggcggca cgctgacggc cgagaatcaa
    15721 cctacgggcg gtgtgcgttt cacgctccgg ctgcccatgc cggacgaacc acccacggtt
    15781 cgggaggcga cccaatgagt tcgggggcgc gggtgctggt gatcgatgat gaggtgcaaa
    15841 ttcgccgctt tttgcgcatc agtctggagg cgaacggcta tgaggtgcat gaggctgccg
    15901 ccgggctgga tggcattagc cgggcggcac aactgcgacc cgacttgatc atcctggata
    15961 tgggtctgcc tgatatcgac ggactggagg tgctgcgacg gctgcgagag tggacgacga
    16021 caccgacgat catcttgtcg gtgcgcgacg ccgacgacga caaggtggcg gcattggatg
    16081 ccggggctga cgattatctg accaagccgt tcagcctgga agagctgatg gcccgaatgc
    16141 gcgtgacgca gcgccacgcc ctacccggct cgcaatcaca tctgttcaag gcgggggata
    16201 tcgcggtcga tctgtctcgc cggctggtca cacgcaacgg agaggccgtg aaattgacgc
    16261 caacggagta cgccttgctg cggctgctca ttcagcacgc cggtcgagtc ctgacacaca
    16321 agcaaatcct gcgcgaagtc tgggggccgg aatacgtgga ggagacgcat tacctgcgcg
    16381 tctatttcgc tcaattgcgc cagaagctgg aggaaaatcc atctctgccc gcgctcattc
    16441 tgaccgagcc gggggttggt tatcgattag ctgcnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnncacgct cgatctcgtg cctcacactt tcttcagaat tggcgagcaa
    16621 atcggcttca atctcatccc gattgagatg gaagtaggcc aacgcagcat agacccccgc
    16681 caaatcaagg gccatctcat cggcgatctc ctcagccgac aagccaagct tgtaatgccc
    16741 gacaaccgtt cgcaccgcga ctcccgttcc gccgatgatc gggcgattct catgtatctc
    16801 aggtatccgc actatcaggc cttccagcat ggaaacagtc atagcgttct cccgacggta
    16861 tctcgacttc attttaccat aggaagtcaa acaagtgtga cgctcaacct gcacgataga
    16921 tcatcccgga gatctcactg caacgaagag agcaagttac cgatcaccgg caatgagagc
    16981 agcagccaca tccccaaccc gcacagcgca aaaatcagca cgagcagcag caacgccagc
    17041 caccatggta attgaggcgc catgaccact tgcccatcca tctccgtcgc cgaaccgggg
    17101 gacgacaagg gcgtgacccg gacgacaaac ggccactctc gcttgcgccc gatgaacggc
    17161 tgtttcatcg gctgcaaata aaaatcgacc acgccttgct ggtttggcac gagactaacc
    17221 tgcttttgcg ccgcatccac gtaaaggccg ccgcgcggtg tctccacagt caacgagata
    17281 gtctgaagcg tgggcgcgtt gttctgcaac aagacgcgac aaacaccctc tccccgcagt
    17341 tcgcgcggcc gcatatccca ttccagccgt cgagcggggg tcggaaactc cgtcaccatg
    17401 ctcacgatgg tcgagccgct atcttcggcg aaggtgcgcg cttcctgatt gcccagcgtg
    17461 gcggccgttc ggcgcagatc ggcggccagc tcctcggccg tctggtagcg attctccggc
    17521 tgccgattca gggcgcgcaa gataatcgtc tcgacagcgg ggggtacccc gacatgcatc
    17581 tcgcgcgggg cgacgatctc ttcatactgg tgggcctgca ccgctccctc aggcgagcga
    17641 atgtccagcg gcagcttgcc ggtagccaat tgataaagca ccacgccgag cgaatagatg
    17701 tcggagcgac cgtcaagcgg ccgatcaagc acctgctccg gtgacatgta gggcagcgtg
    17761 cccatcagca ggcccgattg cgtatcctgc tcaatcccca gcagcttggc cagcccgaag
    17821 tcggtgacca tggctcgcaa agggggatcg ccgggacggt cggcgcggtc gagtcgcttt
    17881 acgaggatgt tgtccggctt cacgtcgcga tggataaccc cctgccggtg ggcgtagccc
    17941 agcgcatcgg ccgcctgggc cacaagggag agcgtctcct ccagccgcac cacctgattg
    18001 agccgggcca gttggcgcaa atacgcgccg aggctcagcc cgtcgatcag ctccatgacg
    18061 atgtagagca acccggactc gcggccgaaa tggtagatgc gcacgatgga cggatgatcg
    18121 agacgagccg cggcgcgcgc ttcctgcaag aaacgctcct ggaatgccgg gtcctcggcc
    18181 agctcgccat gcatcacctt gacggcgacc ggccgcatca ggttgacatc ggtggcgcga
    18241 tagatggtgc ccatcccgcc ctcgccgatc acgccgtcga tgcggtagtt ctcgatttgt
    18301 tgccccggtt ggatcatggg ctaatcatcg cctacgttag acggggaggc aaatttgtga
    18361 ttgacatccc ctattgatta ccgtaaaata aaatatataa aatgtatttg tccacatagt
    18421 tcggaacaat attatgcctc aactccatct ttatattcct gatgaattgg ctgagcgaat
    18481 tcaacgagaa gcacaggcag ccgatatgag catctcgcgc tatttggcgc aattagttca
    18541 gcgtgaaata tcgcccgatt ggccgacagg gtattttgaa gacgtggttg gtggctggaa
    18601 aggcggatta ctggaacggc cgtcgcaagg caccctggaa caatgagagc ggctggaggc
    18661 ggactggtaa ccccatgtac ctgcttgaca caaatgcatg tattcgcatt ctcaatggcc
    18721 aatccatatc ggtggttgag cgtcttcggc gacaatctcc gcgagcagtc tatttatgtt
    18781 caatcgtgaa ggctgaattg atctatggcg ctcaccgcag cgccagaccg gcagagaaca
    18841 tgcggttgct caatcgcttc ttcgaaccat acgaatcgct cccctttgac gaccggtgta
    18901 ctgaggttta cggccgcatt cgtaacgaac tggagcgctc ggaaatggtc atcggcccga
    18961 acgatcttat gatcgctgcg acggccgtca cacatcgact ggtgctggtg acagccaaca
    19021 cccgtcaatt cagtcgcgtg atcaatcttg agatcgagaa ctgggaattg gacaaataat
    19081 gggccggttc gacgcgtggt taatgatcca cgcgccgcca cacgccatct tcgccgacga
    19141 aaataaaaag atcgccaaac ggcgcggcca ggatcgtgcc gttgacgaaa tcctgggcgg
    19201 ccgaggcgct gtcgccaatc tcggcggccg tcacgttgcc cagcgcgcca cgaatcccgg
    19261 gattgtcgca ccagacacgg ccgaaaccgc ggatgggggt gatcgccggg tcttccttgt
    19321 cgcaggtgaa gggcggatca ccctcacgcc acatattagg gaacgatgtc cagcggccgt
    19381 cgttggtcag gacatagatc agatccgtgt cgccgcgcca aagcatttgc ccaccctcga
    19441 agggcgcata agcagtgttg atggctgctg cgccggggtt gatggggcag ccgagcagct
    19501 cgatatccca ggcgtcggcc aggccggcct gaacggggat ttggcaggga gtggcggatg
    19561 gctgtatcag cgtagttgga gtaacggaaa taatcacgat tggagccgcg agtgtcgcag
    19621 gatcgagtgg ctcatcggcc gtcgctattg aaagtgtcgg cgcaagtgtc ggaagagact
    19681 ctgtgcccgc actgccgata catccctgca gaccgaacac aaacaagcaa agcgccagcg
    19741 aaaaggcaag ggatcggggc ttcgtaattc ctacgccccc ttctccaacg ccatcatccg
    19801 ctccggattg cgtccctcga tgccggcgaa cagcgcctga atgcgcggca ggtccgcttc
    19861 gtaactatcg cccggccaca ccatcggccc gacgcgcatc cgcttgttgg cgtagtcgaa
    19921 caagaccatc accagcggca cccccgcccc tttggcgatg tagtagaacc cgctgcgcca
    19981 gcctttcgtc gcgctgtcgc ggtccttgcc ccgtgtgcct tccggcatga tcgccaacag
    20041 gaattgatcg cggtgctcga actcgtccac catttgcgac acgaaatccc gcgatcggtc
    20101 gcggtccagc gcaatgccgc cgaaccagcg caagaaccag cccaccggtc ccacaaacgg
    20161 cgtgtgcttc atcacgaaat gcacgtccac ccccaaggcc gccatcgtca gcgccgtcat
    20221 gaagaagtcc agattcgacg tatggggcgc gcccaccaac acgaaacgcg gcagatcagg
    20281 tatttcgccc accgcccgcc agccgaacag gcgcatcacc agcgaccaaa acccgcgcga
    20341 cagcctattg ccccgccgcg gaatcgccgc ccccacctgg ggtagcgttg tagatgtaat
    20401 atccaccgta tcggaaacca ttggctccat cttcaacaat ctcctcaatc attcgattgg
    20461 gtccggaaac gatccggctc ttctttatta aacccgaaaa cgccccaaaa gaaacgnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct tccgcaacgg tgaccccacc
    20641 aacgacccga cggccgccag ccttgaaggc gcctggcccc atgaccacac ctcgccctgg
    20701 gaagttcatc catgggggtc agactggtac gccgcccagc cctacgagca gcagaacggc
    20761 cgagacatct ggtacaacat ccagcgacgg cgttatggcg gcgacctgca gggggtcatc
    20821 gacagcctcg actatctcgt cgatctcggc atcgaggcca tctatctcaa ccccgtcttc
    20881 gactccccct cattgcataa atacgacggg gccacctacc accacatcga cccccacttc
    20941 ggccccgacc ccgacggcga ccgccgcctc attgccatcg agaacccgat cgaccccgcc
    21001 acctggcaat ggacatccgc cgaccgtctg gccctgcgtc tcatcgacga agcccatcgc
    21061 cgcggcctgc ggatcatctt cgacggcgtc tggaaccaca tgggcatcaa cagcttcgcc
    21121 ttccgcgatg tcgtcgctcg ccaacgcgac tccgtctatc gcgactggtt ccgcatcgac
    21181 agttgggacg accccgccgc gggaaccacc ttcgcctata ccggctggtt cggcgcgcac
    21241 gagttgcccg agattctgca ggacgaaaac ggccacgccc ccggcccgcg tgactacatc
    21301 tacgccgcca cccgccgctg gatggacccc gacggcggcg gcgacccatc cgacggcatc
    21361 gacggctggc ggctcgacgt cgccttctgc atccgccacc cgttctggaa gggttggcgg
    21421 cagcatgtcc ggagcatcaa ccccgaagcc tatctggtgg ccgaggtcgt ccagtccccc
    21481 gacgaggaga agccttatct ggaaggggat gaattcgacg cgacgatgaa ctacaacttc
    21541 ttcatcgacc tctacgagtt cttcatctcc cgggactacc gcgtcaccgc caccgaattc
    21601 gacgccaggc tgcgctacct gcgcgaagct cacccggcac aggtggccca cgtcatgcaa
    21661 aatctgctcg actgccacga caccagccgc atcgcctcgc atatcgtcaa ccgcgacatc
    21721 tggaatattc gcgaatggaa cacattcttc cagcgcacca aaccggccga cacgcccgaa
    21781 ttcgacaccc gccggcccac ggccgccgaa cgccgcatcc agcgcctcgt cgccctgttc
    21841 cagatgacct acattggcgc accgatgatc tactacggag atgaggccgg catgtggggc
    21901 ggcaacgatc cctgctgccg caagccgatg gtctggccgg atatggtcta caacgctgag
    21961 gcggtgctgc ccgacggcac gctccggccc acggccgatg aggtcgcctt cgatcacgaa
    22021 ctccacgact attaccggca gatcatcgcc ctgcgcaagg catcgcccgc cctgcgtcac
    22081 ggcgaatacc gtactctatt gaccgacgac gcccgtcatc tcttcgcctt cgcccgcgag
    22141 acggaagatg acgcggctgt atgcgtattt aatgccgacg atctgcacca tatcgtcgag
    22201 ctacccgtcg gcgacggcca atggcgcgag ggcttcacac cgcaagagtc ttccgagacc
    22261 tctgaggtat tggatggtgt tatccacgtc gccatcccgc cccactccgg catcatcttc
    22321 cgccgcactg tggagccggt ttcctaaccg gctgcctttg ctgctgttgt tgagaagatc
    22381 gccgcataag aatccggcgc cacagtcggt cacccgtagg gcgctgccct gctgtggcgc
    22441 aacctgtcca ggttgcgtct gccccgcaat cgctgtcgcc atcgaagatc gatggcgaca
    22501 gcgattacga tagcgatagc ggcgaagaga acccacccct accccggatt ctgcgaatag
    22561 atcaccccgc tatagggggc cacattcacc tttccatgcg ccggcaggcc atcatatgcg
    22621 ccttcctcgg ccacgacatc gacgctcacc tgccccttat agtcggggct atagccggcg
    22681 gcgtggctgt cgaatcgcag cgaccatttc cccgcagccg ggaagccaat cacgtagtcg
    22741 gtcaacgtgc ggttagccat gttgatcacg acgacggtgc tgtcacccgg cccaccgtca
    22801 taccagcgat ggtaggccag caccttgtcc ttctgattca tgtggtagac cttgatatgc
    22861 tgcccggtca ggccgcgcgt cgtgcccttc aggttgcgcc gcaggtgcat cagatcgcgg
    22921 tagagataga cgatcccctg ctcttccttg acatattccc aatccagcgg cacggtatcg
    22981 gtgaaccact tgtcctccag gaactcctgc ccctgaaaga tcatcgggat gcccggcgcg
    23041 gtgaagacca gcaccgcccc cagcgtcgag cgcttcttgg cataccaatt atggctgttg
    23101 cccggccata tctcctccgg cacgcgggcc ttgccgttgg ccacttcgtc gtgggactcg
    23161 gtataaacca cccgcttgaa cacgtccccg gcatacttga aggtcagtgc ctttttcacc
    23221 gccttcatgt cgcgaaaagc gtcatcgctg gagatgatgg ccgtccgcac cgggtggacg
    23281 aagttggcgt cccattgcga tccgaacccc gcgccgcccg cgccggtatc cttcacgagc
    23341 cactcgttgt ttcgcagatc ctcggcaatc gtcagattat tcggctcctg ctcgtgcact
    23401 tcctcgttga tccactgcat caggctccag ccatcaggca gatcgttggc cggttcgttg
    23461 tcgttgccga agatattgcg aatgtaggcc gtcgcatccc agcgcaaccc atccaccttg
    23521 tactcgtgca gccacatcag cgcattatcg cgcagcatct ggcgcacttc ggggcggccg
    23581 tagtccggcc gggtgtggcc ccatggcgtt tcgttgcgcc gatcgttata gaaatagata
    23641 ccgccgccgt ccccctccga ccaaccgtcg aatcgccaca gatccagatc gcccggcccc
    23701 agatggttgt agaccacatc gaggatgacg gcgataccat gcccgtgggc tgtcttcaca
    23761 aactgcttga agcccatcat cccgccgtaa tccgtctcca cggcgaaagg cagggccggg
    23821 ttataacccc acgaacggcc gccggggaac tccatcggcg gcatgatctg caccgcgttg
    23881 acgcccagcg ttcgcaaata cgagagtttc ctggccgctc gctggaacgt gcccacccgt
    23941 tcttccgaca tcgggttgaa cgtgccgata tgcatctcat agatgattag ctcgttccat
    24001 gacgcgcttt ggaagtttcc ggccccgccc cagtcgaaat tggggtcatg gatgaggctg
    24061 ttgccgacgg agctggtcac ctgccgcgag taaggatcga ggcgatggag tcgctgctcg
    24121 ccattgacga taatgtacct gtattcctgg ccgggtttgg ctccggccac gtcgattgac
    24181 caatagccgt cgacctcatg taccaatgga ttggcgtttt ccttccaatc attgaaatcg
    24241 ccgatgacgt acaccgcttg cgcgttgggc gcccaaactc gaaaggccgt tcccttggcg
    24301 tgggcgatcg gccccatgcc cgccttcagc gccggccgcc ggccgcgatc ataagcctta
    24361 aatcgctccc caatggcctt cttcactgtt gtgccaatat ccttcaacaa atagtttcac
    24421 tcctctccgg caaccgcagc gcagtatttc ctgcctcgac cacaatcgtc ttatcggtgt
    24481 ccggatcaca ctctgattct ctacattata gcaaacccaa agaaggcgca ggccttctca
    24541 tatcctcagg ccccccttct gccgtgcggt gttctccatc ccggcaacga cattcgggca
    24601 accatgattc ctcgccggtg atgtggagag gggtcgcggc ccgactgcgt cataatggtt
    24661 gcgctacgtg ggttgcgcta caatacgtgc gttcgataac ccccggcaca ggccggatcc
    24721 gacggatgat gagaggaact gatgcgcgtt gctgtgttgg cgaatctgat taaaaatgca
    24781 ccgacgtggc cgggtatgcc tgaggatcgc tgggatgacc tcgattccga ggagactatc
    24841 gccgccattg tcgcagcgct ggagacgggc ggccacgatg tcaccttcct ggaaggcgac
    24901 ctgtcgcttt tcgaaacgtt acccaagttg aagcccgata tctgcttcaa catctgcgaa
    24961 gggcattggg gcgattcccg cgaggcgcag gtgccggcca tcctggagat gctgcgcgtc
    25021 ccctacaccg gctccggccc gctcacgctg gcgttggctc tcgacaagcc aatgaccaag
    25081 cgggtgctat ggtaccatag cctccctact cccactttcc agaccttcga gcgcgttcac
    25141 gagccgctgg accccgatat gacctttccc cttttcgtga agccaagccg cgagggcaca
    25201 ggcatggggg tttcggccga ttccatcgtc cataatgagg agcaacttcg cgcgcaactg
    25261 cgctaccaat tcgagaagtt cgatcaaacc attctggtcg agcatttcat cgagggacgc
    25321 gaagtgacgg tcgggctggt gggcaatcta tccacgccgg ttgcgcgccg tctgcccgac
    25381 gacctggaag cggcgcgcat ctcacgcggg ctgcatttct tcccgccgct ggaagtggac
    25441 atgagccgct atccggtcga ggaagcgggc gtctatacca gtcgcatcaa gaccgagttg
    25501 gtgcatacgt tccactatct gtgccccgcg ccgctgcccg aggcgaccat cgaagagcta
    25561 aactggctgg ccgcggccac gttccgcgtg acgggcagtt ttgatgtcgg ccgcattgat
    25621 ttccggctcg atgccaacga caataacaag ccgtatattc tggagatcaa cccgctgccg
    25681 gggctgaacc ccggctattc cgacctgcct ctggaggcca aggccgccgg gtggacttat
    25741 gaacaattga tcaaccgcat cctggaagaa gcggtggatc gttatgggct ggaggaagac
    25801 gcttcccggc ccgcctgagg tttccgttcg agaggagaaa tatcgatgcc tgcgaaagac
    25861 ccttccctca tggcgcgctg tcttgaatgt gactcgcgta tctatttcga gcgcaaaccc
    25921 gaccccgggg aaatcatcgt ttgtccagag tgcgaaacga gtctggaggt gatccggtca
    25981 tcgccaatca agtttggctg gcccgacgac gccggccagg gcggcagttc catccctcgc
    26041 gtcgatctgg atgagttctt caacgaactg gctgacaacg agagcgagga cgaggattac
    26101 taaaagaggg ttcagagatt
//
LOCUS       DAOMQG010000012        25676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_830_length_25676_cov_11.335974, whole genome shotgun sequence.
ACCESSION   DAOMQG010000012 DAOMQG010000000
VERSION     DAOMQG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 25676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25676
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_830_length_25676_cov_11.335974"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(295..457)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(530..2677)
                     /locus_tag="PLC06_01650"
     CDS             complement(530..2677)
                     /locus_tag="PLC06_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89178.1"
                     /translation="MKNNYSWETDDEADWEPSPTPVVTSSRGKLYTLLLLIAGVAVIG
                     ILILLLLNRRLEGREDIIRQNVIAAQHTWEQAVERQDLELFSTLISRKQADWYQSQRR
                     LLMSGRALDREALGLALLPVETNQFEVELDANWRKAELTFPRQYGVLGVDDQPPITLY
                     QTLTYQISGDRWQLAQPSVSFWGDSVVEDVGMLHITYPERDAEFISRLASNLARDLAA
                     ICAESSAARECLPDSLIPVEFSTDPESLLALDDINTPAMNGRTLMLPAPSLVGLPADA
                     AAYDALYRGYTERIREMLRNDLALPVPLPEQDIVALCFPAFDEGTALYSYNPVSDQWA
                     QQSTPRRFSSLQALPDDSGLVFRVGLPGTYIAHLELVLQQAGSELTLVNEGTTELSAR
                     LNAIPSRPQSDSLIYSTVQGSTGITNYRLLPFDSCVDGACDAIPLAGFPVWSPDGEKT
                     LVQVGSQLHIGDSEGEPQELVDRAFSPFWLTDDTFGFIRLVGSSTAESPEMELVIRSV
                     TTEQERLLVRSADLMSQLDPNRSGAFRIMYGTGNPANPNQMFLAGTPIGGAQKQFFVI
                     KVQLDGSSGFPSSDDHDPKLSVILDLGDLPVGDSHTLTPTGYPPFVVSPDGRWLMVVR
                     FVDSITNTWQLYLYNTESQEMKTITFNYPVYPAPFPFYDWSADGNWLLLVDNGFLRLI
                     APDYDYERVITHEFAACRYPAWINRDVLATSDQ"
     gene            complement(2756..3322)
                     /locus_tag="PLC06_01655"
     CDS             complement(2756..3322)
                     /locus_tag="PLC06_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89179.1"
                     /translation="MVDQLTPPPSPAANGQAYRAYLERALEVLDSLTVESYYPSPMPD
                     AADDALAEIVSAFTAWPLPVREQFLAQLPSNKRSLFGIFGHRAATLAVRRGDRDLLRL
                     GLVGNLIANSPIPPNRNIEAALAVFHHCARKLEIEPGEFFEEATQFADEAMANRLTAF
                     GRRSDVTLKQYGWREIKTPEGVRYKFEW"
     gene            complement(3436..5004)
                     /gene="gpmI"
                     /locus_tag="PLC06_01660"
     CDS             complement(3436..5004)
                     /gene="gpmI"
                     /locus_tag="PLC06_01660"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRO89180.1"
                     /translation="MKPYRPVALIILDGWGIREMEHGNAVVQAHTPNYDRWMREGERA
                     VVDASGEAVGLPDGQMGNSEVGHLNLGAGRIIYQDLTRINLSIDRGDFYTNPTLVETL
                     EGVKSRGGKLHLIGLLGEGGVHSYSKHMYALLQAAEQHGVAPILHVITDGRDTPPESA
                     LGYARKLETYLAEGHPGTIASVTGRYYIMDRDKRWQRTELGYQDVVYHEGYRGDDGTM
                     RSAPSLSKALEASYKNGVTDEFVLPVAIDPANPEDRQNVLVEPGDAIIFFNFRADRMR
                     QILAAFIKPAEENFPHEHIPDLDIVTMTNYDSTFPVKVLYPETDITNPLAEVLSDAGL
                     KQFHAAETEKYAHVTYFFNGGVETPFPGEERHLEPSPKVATYDLQPEMSARPLADAVI
                     DRVRTHDDDFILVNFANPDMVGHTGVLAAAIKAVETVDECAGRLVKAITDKGGVAIIT
                     ADHGNCERMINEVTGEPHTYHTTQPVSLFVLGDRYYNLRPRGILADVSPTILHLMGLP
                     QPSDMTGRSLIDEA"
     gene            complement(5090..5161)
                     /locus_tag="PLC06_01665"
     tRNA            complement(5090..5161)
                     /locus_tag="PLC06_01665"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5127..5129),aa:Asn,seq:gtt)
     gene            complement(5223..5435)
                     /locus_tag="PLC06_01670"
     CDS             complement(5223..5435)
                     /locus_tag="PLC06_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRO89181.1"
                     /translation="MSVYKMIEIIGTSKTSWEDAARVAIETAGESLRDLRVAEITKLD
                     VRLDDGEIVEYRARVMISFKFEGADD"
     gene            complement(5526..6164)
                     /locus_tag="PLC06_01675"
     CDS             complement(5526..6164)
                     /locus_tag="PLC06_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRO89182.1"
                     /translation="MPTYFVTIAGNIGVGKSTLVTLLSRKLGWEPVFEAVAENPYLAD
                     FYDDMNRWSFHSQVFFLTRRLQQHHSLLQQPSAVLQDRSVYEDAEIFARNLYMQGHLS
                     ERDWASYFELYQTLAQLLKPPDLVIYLRASTATLRRRIAGRGREYERNIADTYLQDLN
                     RLYDEWAAGFRLSPVLTVDTDNLDYVQYDEHLEQIWSRISDRLHGREYLALK"
     gene            6349..6660
                     /locus_tag="PLC06_01680"
     CDS             6349..6660
                     /locus_tag="PLC06_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89183.1"
                     /translation="MRVLIDRDLCDASLPFCQRCSAAFIRNPEGIDRHCIREIIDDGQ
                     DLLTIQMLTDGRELEIELSEEERELAAIEGWDVIAQFDPALFRTGADKRWRELRMLSI
                     R"
     gene            complement(6730..7020)
                     /locus_tag="PLC06_01685"
     CDS             complement(6730..7020)
                     /locus_tag="PLC06_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRO89184.1"
                     /translation="MSTTWPWYWNWLIAAGVVTFVFYAYDKISAKAGGGRIPEILFHL
                     MSLSGGFAGALLGMLAFRHKTNMRAHPLFLPAILIGAALWSVIIYRLLVTAG"
     gene            complement(7137..7493)
                     /locus_tag="PLC06_01690"
     CDS             complement(7137..7493)
                     /locus_tag="PLC06_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89185.1"
                     /translation="MTPENDQYEDSPFAAILRGELEGTVIARDDEQKFAIITSMEPEA
                     AIHWLAVPFESGFGTSEMQEIHSDRFMALLDYAMAQTRARIAEYPALENGFTIKFHCG
                     AYETVPHAKFHILSIE"
     gene            complement(7553..8437)
                     /locus_tag="PLC06_01695"
     CDS             complement(7553..8437)
                     /locus_tag="PLC06_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRO89186.1"
                     /translation="MTVAIIVHGGAGAWSASDNRLEQAKAACAEAAEAGRAILLKGGS
                     ALDAVEAAVNVLEDCPVLDAGRGSYPNAEGDVEMDALIMDGRDLSMGAIAAIQRVRHP
                     ISLARRVMAESGHNFLVGLGANAFADSIDFPRCSVEELLVHNLHLDEIITSQSADQAA
                     IGDTVGAVALDMAGNLAAATSTGGTANKRAGRVGDSPLVGSGAYADNWTAAVSATGYG
                     EALMRVVISKRVCDFVGTGLSARSACESAIRVLEERTGGQGGLIAVDARGQVGWAFNT
                     RAMPYAYGVDGEPVIAES"
     gene            complement(8596..9774)
                     /locus_tag="PLC06_01700"
     CDS             complement(8596..9774)
                     /locus_tag="PLC06_01700"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRO89187.1"
                     /translation="MREAVIVAGSRTAVGKSKKGTTRNWRSDEMAAAVIQDLLRKAPE
                     LDPIEIDDVIIGCAMPEGAQGLNFARTIALRAGLPVETPGQTVNRFCSSGLQTIAMAA
                     ERIIANGADVIIAGGAETMSMVPMTGFRVNPNPYMVENMPEVYMGMGLTAERVADEYE
                     VSRQAQDEFALNSHLKAAAAYERGAFREEIVPLVIEEVSAGPDGPVRMTTLFDRDEHL
                     RPDTTLEGLAKLKPVFKQGGSVTAGNSSPLSDGAAAVIVMERSKAEALGLTPILRFVG
                     FNVGGVRPEIMGVGPIAAVPRLLNRVGMGLADIDLIELNEAFASQSVAVIRSLGLDES
                     RVNVNGGAIALGHPLGCTGAKLTVQLMHEMRRRNSQFGMVTMCIGGGMGAAGIFERLN
                     "
     gene            complement(9866..12259)
                     /locus_tag="PLC06_01705"
     CDS             complement(9866..12259)
                     /locus_tag="PLC06_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRO89188.1"
                     /translation="MTYRIDKVGIIGAGTMGGGIAAHLANIGIPVVLLDIVPPDLSEA
                     EKNNPAARNKIINSLYDRMTKARPANLARADRGDLITLGNLEDDFDKLADCDWIIEVI
                     IEQLAPKQALMKRLEAIRKPNAIVSSNTSGIPIGQIAAGRSDEFRAHFLGTHFFNPPR
                     YLKLLEIIPTTDTDPEVVEFMKRFGSEVLGKGVVVCKDTPNFIGNRFFAVASSFGLEH
                     ALQNGYSIAEVDAITGPTIGRPKTATYRLLDLVGLDVMAHVNNNLYDAVPHDAYREML
                     RPEKTSALMTAMLEKKWLGNKAGQGFYKQERVNGVREFWTLNPATMAHEPTPKVRFDS
                     IGAVRNVEDLGERVRKLLTFDDRAANYVRDILYYGFAYAAYVAPEIAYHLSDVDDAMR
                     WGFSHEAGPFEMWDMLGVAETAEKMEAQGLRVAPWVKEMLAAGHKSFYKRNGATQVYD
                     FNAKAYRAAEPDKNIILVSDLRAAGKTVEHNDSASLLDMGDGVALFEFHAKMNAIDQD
                     IIDMAFKALERLDSDFDALVIGNNGEHFCAGANLFAVGVAAASGHFDALDKMIKALQT
                     ATFNLRHAPKPVVTAPHQMALGGGVEMAMAGWEAVADHETYMGLVEFGVGLIPAGGGC
                     KEILRRKVNPTMKIANADVLPPLQEAFEQIALAKVGTSAWEARAMGYLTPQDTIVMNS
                     DHRLAKAKQRALQLVEFGVRPPEVEKICAAGRDALYALKLGVRSLHWGGYASEHDAKV
                     AGKLAYVLTGGDLSGPTWVDPWYILDLEREAFLSLLGEEKTRERMMYMLQTGKPLRN"
     gene            complement(12377..13687)
                     /locus_tag="PLC06_01710"
     CDS             complement(12377..13687)
                     /locus_tag="PLC06_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO89189.1"
                     /translation="MISFEIPPFIENQLQMIRMVAEQVMRPYSRQLDENEHQRPEAFV
                     QMTWPFTQQMVKTSLKPLMAHSNGSNGNNGSNGANGSNDVNGSAKPKREGPDFGMLSF
                     IMMIEQLSWGDAGQYLCLPHPMLGGAAVDSAGTPEQRVRFLKKFTEGDPKWGAMAITE
                     PGAGSDNSAMRTTAVLDPETNEWILNGEKIFITNGALALKESDGLCVVWATVDPKAGR
                     AGIKSFVVEGNTPGVTIAKQEHKLGIRASDTVALHFNNARIPFENMLGDQEAREMEGR
                     KGFRGAMKTFDASRPAVAASAIGIARAALEFTKSKLVEEGIVIDYTKSLHEMTAVERD
                     IIEMEARYKGAWLLTLRATAQMAHGESNRLEASMCKYRAGDAVTWITQKAVELLGPMG
                     YSREWLVEKWMRDAKINDIYEGTKQINQLIVARSILGYSRRELK"
     gene            complement(13721..14812)
                     /locus_tag="PLC06_01715"
     CDS             complement(13721..14812)
                     /locus_tag="PLC06_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO89190.1"
                     /translation="MLDFRPNEEQKMLAETISRYSRERVRKVFRDAEEEGDIPEAVIK
                     AGWEIGLLPAALPEEYGGFGDYSAVTGALAFEEFAWGDLAVTLEVMTPNLVAIPLLLC
                     GTDEQKAQFLPRFCEETPPKMTAALTEPAVQFSPYKLRTTAVRDGDAYVLSGTKTFVP
                     QANDAEYILIYAAEGGRTQAFLVPAGAEGLTVGGRNKLMGIHSLPAYLVTLDKVRVPA
                     ANKLGGENGIDFSLILNHSRVALGAAAVGMARAGFEYARDYAKERVQFGEPIAHRQSI
                     AFMLADMATEVDEARLLVWEAAWKLDQGHDATPDATIMKQQVDRMVMQVADRAVQILG
                     GYGYIREYPVELWLRNARGFATFDGLAIV"
     gene            complement(14851..15717)
                     /gene="folP"
                     /locus_tag="PLC06_01720"
     CDS             complement(14851..15717)
                     /gene="folP"
                     /locus_tag="PLC06_01720"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRO89191.1"
                     /translation="MFQWGTRTYVMGIINVTPDSFSGDGVAVGTGEKLVERAVEQAHR
                     FVEQGADILDIGGESTRPGSKPVTADEEVSRLIPAIQAIRSEIGDVVISADTYRADVA
                     ELALDAGANWVNDVWGLRMDPDMAGLIARRGCPIVIMHNRSKPKDVAQETRLGGRYVG
                     VAYGDLLGDIKAELTEQIAVALAAGVRHEQIIIDPGIGFGKTVEQNLELLDRLGELKA
                     LGYPLLSGPSRKSFIGYTLDLPPDERMEGTAAAVAISIDRGADIVRVHDVAEIVRVAR
                     MTDAIVRRRAGQ"
     gene            complement(15744..16268)
                     /gene="folK"
                     /locus_tag="PLC06_01725"
     CDS             complement(15744..16268)
                     /gene="folK"
                     /locus_tag="PLC06_01725"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRO89192.1"
                     /translation="MSRIYLGLGSNLGDRYANLQHAVDELRQFVTVTAVSPVYSTEPW
                     GDTDQPAFLNICVAAISSLPPHELLADIKKVERELGRTPTRHWGPRLIDIDILFYDDL
                     VLSDETLTVPHPFIADRSFVLVPLADIIPDFRHPQTGLSVQEMLDKVDSSGVERLFEM
                     PFPGEALPTDASPA"
     gene            16437..17687
                     /locus_tag="PLC06_01730"
     CDS             16437..17687
                     /locus_tag="PLC06_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRO89193.1"
                     /translation="MRTVIALLILAMTALGANNVSVEAQQPLPHVVDPGDTWPALAMR
                     FGVEESELRAMNPQFNTLRQPAIGSTISLPEEASEQPGRLVRDGDAGILAIALQYNVS
                     PWSLALQNELDSPYRPTFYRPLFVPTEGTIRDLPQGIDSLEVSMSPALPGVALGIRGT
                     SQQETINLNGHLGGLPLAFAAENNRFIGVAGTGAFFTGGEPELVLKSGDAPAWVQPWQ
                     FAKREWIYQELTLTGEAAQIDQNARDEERARLRELWGQITPEPLWQGPFVTPVTTYLE
                     ISAEYGARRSYNGGPYLTYHEGVDYSAYGGTPVMAPAAGKVVLAEPLYVRGGTVIIDH
                     GLGIFTGYYHLSAIHAIAGQAVEPGDLLGEVGTTGLSTGNHLHWDLLINGIWVDAAAW
                     QEQRMDCWLLEGLGRSCATEQPAD"
     gene            complement(17692..18693)
                     /locus_tag="PLC06_01735"
     CDS             complement(17692..18693)
                     /locus_tag="PLC06_01735"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRO89194.1"
                     /translation="MSRIVQSLSQSDVRVFAGRSNPGLAKSIADNLGVRLEETRVSRF
                     SNDNLFVQLGASVRGRVVYIVQSLVPPVSDHLVELLMMLDIAKSAAAREIHAVIPYFS
                     YARSDKKDAPRISITARLVADLLNTAGATHIMTMTLHSPQVHGFFSVPADPLTARGLF
                     ADYFRARQYSAEDTIVVSPDVGSAKSSVRFADMLGLGVATANKTRIDDTRVQYSGLIG
                     RQVSGFKRAIIHDDEIATGGTIVELARLLLDHGLEEISVVCTHGVFLHGALARLEAVP
                     EIREIVTTDTVPQNSSDQSRLVVLSTGPIFAGAIRQNYFRRSIGELFDFGDRSVTEE"
     gene            complement(18807..19388)
                     /gene="aat"
                     /locus_tag="PLC06_01740"
     CDS             complement(18807..19388)
                     /gene="aat"
                     /locus_tag="PLC06_01740"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRO89195.1"
                     /translation="MDLTPELLLTAYAQGIFPMADEEGQISWYSPDPRAILPLDRLHV
                     SHSLRRVIRSGRYDVRFDSAFTEVMAACAAPAPDRELTWISDELIEAYTWLHELGFAH
                     SVEAWLGDELVGGLYGVSLRGLFAGESMFSIRSNASKVALYYLVEHLLGRGFTLLDVQ
                     FQTAHLAGLGSVEVSQARYRYLLKQAMALDARF"
     gene            19523..20683
                     /locus_tag="PLC06_01745"
     CDS             19523..20683
                     /locus_tag="PLC06_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRO89196.1"
                     /translation="MYDVESLRREEFPATATLTYLNHAGISPLPQRTKRAVQTAVERL
                     SEEPSAFFGKYAQPAFATLQESLARYINADLPSEIVYSTSTSAALNGVAQAIDWQPGD
                     NILFCDQEFPSNAYPWMSLARDGVESRRVPSEGGGLTLRQVEAHADDRTRAVAVSAIQ
                     FFSGHRTDLVAIGRFCHEHNILFIVDAIQAIGHMVIDVQAMNIDVLATGGMKSLLALP
                     GAGFIYVRDEVSAKMNPRLIHGNSTVDYIHWLAYDLTPQPGAARLSSGTTNVPGILSI
                     GASLTLLNELNPADIDAHTTALIHYAIDGLTRDGYEVITPKSASGPIATFRSPFDSET
                     TDRLVQFLSDRQIIVCKHLDAGTSAYVRASVHCYNTAADIDRLLEGLRGFVN"
     gene            complement(20676..>21206)
                     /locus_tag="PLC06_01750"
     CDS             complement(20676..>21206)
                     /locus_tag="PLC06_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRO89197.1"
                     /translation="DGVATRVYVMTAWVCPFEGVISPLKTMATVQEIADWGVDEIAIA
                     DTIGHADPLSVGRLIDRIARRVAIERLAVHLHDTQALGLANATTALQAGIRTFDSSVG
                     GLGGCPFAPGAAGNLATEDLVFLAFKVGLGTGVDFARLWDVVYDLERMIGRPIGGRIR
                     QWWESSRDHEPRVDFN"
     assembly_gap    21207..21306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21373..23277)
                     /gene="acs"
                     /locus_tag="PLC06_01755"
     CDS             complement(21373..23277)
                     /gene="acs"
                     /locus_tag="PLC06_01755"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRO89198.1"
                     /translation="MSSEKGLTGEVYYPAPEVSAHAHIKDYDKVYAEAAADPAAFWGR
                     IAAENFEWYKPWNTVLDDSNAPFYKWYDGAQVNIVHNALDRHLRTSVRNKAAIIFEGE
                     AGDSRTYTYQQLSHEVSKFAGVLHSQGVGRGDRVTIYMGRIPEMVVAMLACAKIGAIH
                     SVVYGGFSTEALYDRIVDSNSKVLVTCDGAWLRGEVVELKKIVNESLEKANPIQSVIV
                     VKRTGREVEMKAERDHWYHDLMALPIADPKTPTEHMDAEDPLFILYTSGTTGRPKAIL
                     HTHGGYMVGTAITLKWVFDVKPHDIYWCAADPGWITGHSYIVYAPLVLGATSFIYEGA
                     PTYPNPDRWWAMVEKYGITILYTAPTAIRGLMRFGESWPGLHDLSSLRLLGSVGEPIN
                     PEAWRWYYRVIGKERCPIMDTWWQTETAMFMITPLPTMPLKPGSGAKPFPGIKVDVVN
                     EEGESAAPNEEGYLVIQTPWPAMLRTIFGDPDRYVQQYWSRYDEQGWYLPGDSARKDE
                     DGYIWVIGRIDDVIKVSGYRLGTAEIESALVSHPAVAEAAVIGVPDQLRGHIIKAYCI
                     LRLGYEPSERLTRALIDHVGHEMGPIAKPSSVEIVDSLPKTRSGKIMRRVLKARALGQ
                     DEGDTSTLES"
     gene            23382..24767
                     /locus_tag="PLC06_01760"
     CDS             23382..24767
                     /locus_tag="PLC06_01760"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89199.1"
                     /translation="MSKRKIVIIGAGSASFGPTTLATIVRNEALRGSELALVDLDRTA
                     AETAAWVARRMNESWQADMTISSTTDRREALPGATHVVVSIEVAPREEHWRLDWEIPL
                     RHGLRQPYAENGGPGGMMHTFRQVPAHMAIARDMEVLCPDAWLILFSNPLPRLCRAIS
                     KYTRVKVVGKCHQINIGYALAAALLHRDYNIPVPEGVVLHSDPGNAPTIYTLSHAGRK
                     HFEITSAGLNHFIWLLDIRDRRTGEDLYPRLREAIDLAPPTLEPLALELFRTFGLLPI
                     AGDTHMAEYLPWLHDPVAGPWETYQLPLYDWTGNESVREALHSLMAAMGSGDIPVDGM
                     REAVSEGATELVEALGDGDSYVDETVNVPNRGAIAGLPHETIVEVPAYISHTGIRPLQ
                     IGALPYPVTELCRREAALVELVVDAAVTGDRSLALQALLLDPMITDIGSARAILDDYL
                     TTFADYLPQFQ"
     gene            complement(24801..25334)
                     /locus_tag="PLC06_01765"
     CDS             complement(24801..25334)
                     /locus_tag="PLC06_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89200.1"
                     /translation="MIWYDLIQPYLLPALGISGGVLLLLWLAVVIWAFRDIRARSRSG
                     WAVFFTVLLIALVPIVGLVVYWLLRPRETLSAAYDRALEQEALLQQIEERPVCPTCSR
                     PTQANWFLCPTCHTHLRQPCPVCQSPLELHWDICPYCGYLFPDETADDDAVAPMSFAE
                     EEAVAPVQSFDEMDDRS"
     gene            complement(25436..>25676)
                     /gene="rpsU"
                     /locus_tag="PLC06_01770"
     CDS             complement(25436..>25676)
                     /gene="rpsU"
                     /locus_tag="PLC06_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRO89201.1"
                     /translation="RCQNYKKKGVNTLPKVTLRSDESQEQLLRRFSREVVKSRLLTDV
                     RRKRWFVSKSELNRIAKKKASRRTRKSQKEQQQGV"
BASE COUNT         5682 a         7600 c         6955 g         5339 t
ORIGIN      
        1 acggccgcct gaaccgacga ccgctgatca gggaaagccg gcgcgcttgc agcttcggga
       61 ttagccagga gcgcatcact ccgcccaaaa agcagagcca ggacagcaaa caaaaccaca
      121 ataatcagaa cacgattttt tatcattgtt tcctcgtctc tccgtataac tatcgctgtg
      181 tcgggataac gagggagggg aaacaggtaa caaaaaactc gatggtccgc catcgagttg
      241 agaattttgg agacaaattg ttttgccact aagttcccaa cccttatccg cgagggcaga
      301 aaactcatct caggatgtcg acctggctta ttgagcgcta agtctcgctc aaatcacagt
      361 tgcgggaaca gcatcggcct tgctcatggg agcgcaccga tttcgcgatt acgccctgcc
      421 ttccggggca cgggcgacct gagatggaat ctatatttaa ttgtatcggc agtctatcag
      481 atggatccgg gagcgtcaag ccgtctgcgc ttcgttcact cccctcgaat tattgatcac
      541 tcgttgccaa cacgtcccga tttatccagg ccggatagcg gcaggcggca aattcatggg
      601 tgatcacccg ttcatagtca taatcgggcg cgatcaatct gaggaaacca ttgtcgacca
      661 gcagaagcca attgccgtcg gccgaccagt cgtagaaggg aaaaggagcc ggatagacag
      721 gataattgaa cgtgatggtc ttcatctcct gactttcggt attgtagaga tacaactgcc
      781 atgtattggt gatcgaatcg acaaagcgga caaccatcaa ccacctgccg tcgggtgaca
      841 caacaaatgg cgggtagcct gtcggggtaa gcgtatgcga atcgccgacc gggagatccc
      901 ccagatcaag gatcacgctg agcttgggat catgatcgtc cgacgagggg aaccccgaag
      961 agccgtcaag ttgcaccttg atgacgaaga actgcttttg cgccccacca attggcgttc
     1021 cggctaaaaa catctgattg ggattcgccg gattgcccgt gccgtacata attctgaacg
     1081 cgccggaccg attggggtcc aattggctca tcagatcggc cgatctgacg agaagccgtt
     1141 cctgttcggt ggttaccgac cggatcacta actccatttc ggggctttcc gcagtagaac
     1201 tcccaaccaa acgaatgaac ccaaatgtgt catcggtcaa ccagaatggg ctgaatgctc
     1261 ggtcgaccaa ttcctgcggc tccccctcgc tgtcgccgat atgaagttgc gatccaacct
     1321 ggacaagcgt cttctcgccg tcaggggacc acacaggaaa accggccagc ggtatggcgt
     1381 cgcaagcgcc atcgacgcag ctatcgaacg gtaaaaggcg ataattggtt atccctgtgc
     1441 tgccctgcac agtactataa attaggctgt cactttgcgg ccgcgacgga atggcattaa
     1501 ggcgcgcgga tagttcagtg gtgccctcat tcaccagcgt cagctcgctc cccgcctgtt
     1561 gcagaaccaa ttccagatgg gcaatatagg tgcccggcag acccactcga aaaacaagcc
     1621 ctgagtcatc agggagagcc tgtaaagagc taaagcgcct tggcgtcgat tgctgtgccc
     1681 attggtcgga gacaggatta taggaataca aagctgtccc ttcatcaaaa gcagggaagc
     1741 aaagcgccac aatatcctgc tcaggcagcg gaacaggtaa agccagatcg ttgcgaagca
     1801 tttcccggat tcgttcggta tagccgcgat aaagcgcgtc atatgcggca gcatcagccg
     1861 gcaggccgac caatgaaggc gcgggcagca taagcgtgcg tccgttcatg gccggcgtgt
     1921 tgatgtcgtc caatgcgagg agtgattcag gatccgttga aaattcaaca ggaataagcg
     1981 aatcaggcaa acattcgcgg gcagcggagc tttctgcgca aatggcggcg agatcacgtg
     2041 ccagattcga agccaggcga ctaataaatt ctgcatcccg ttcgggataa gtgatatgca
     2101 acatgccaac gtcttcaacg acggaatcgc cccagaacga aactgagggc tgcgccagtt
     2161 gccagcgatc gccgctgatt tgataagtca gggtttgata gagcgtgatc gggggctgat
     2221 catccacgcc aagtacgcca tattgacgag ggaaggtcag ctctgctttc cgccaattgg
     2281 cgtcaagctc gacctcgaac tgattcgtct caaccggtaa cagcgccagc cccagcgctt
     2341 cgcgatcaag cgctcggcct gacatcagca atctgcgttg ggattggtac cagtcagcct
     2401 gcttccggga aatgagcgtt gagaataact ccaggtcctg ccgttctacc gcctgctccc
     2461 aggtgtgttg ggcggcgatc acgttttggc gaatgatatc ttcccggcct tcaagacgcc
     2521 ggttgaggag gagaagtatc aaaataccga tgacggcaac accggcaatc aataataaaa
     2581 gagtatataa ttttccccgc gatgaggtaa ccacaggggt gggggaaggt tcccaatcag
     2641 cttcatcgtc cgtttcccaa ctataattat tcttcatttg ggaactcagg tttgccgtcg
     2701 atggcgagca agcaattagt cgtgcctgct atcacgggtt atttggcagt tttctctacc
     2761 attcaaactt gtagcggacg ccctccgggg tcttgatctc tcgccagccg tattgcttca
     2821 acgtcacatc cgatcttcga ccaaacgccg tcagtctgtt cgccatcgcc tcatcagcaa
     2881 attgagtcgc ctcttcgaag aattcgcccg gttcaatctc aagctttctc gcacaatggt
     2941 gaaagacagc cagggctgcc tctatatttc tgttaggcgg gatcggcgaa ttagcaatca
     3001 ggttaccgac caaccccaac cgaagcaaat cacgatcgcc acgacgaacg gccagcgtgg
     3061 ctgcccgatg accgaaaatt ccgaacaggc ttcgcttgtt tgacgggagt tgggccagaa
     3121 attgctcgcg caccggcagg ggccaggctg tgaaggcact gacgatttcc gccagagcat
     3181 catcggccgc atccggcatg ggagaagggt aatacgattc gacagtcaga ctatcgagga
     3241 cctcaagcgc gcgctccaga taggctcggt aggcctggcc atttgcggcc gggctggggg
     3301 gtggggttag ttgatcgacc atgttggctt atttatacat ggtcggattc cataagccaa
     3361 caggtttgtc atgaatagcc aatcggcccc ggagcttgct gcagtttctc tccggggccg
     3421 ttaatattgc cgaatttaag cctcgtcgat gaggctgcgt cccgtcatgt cggaaggttg
     3481 aggaagtccc ataaggtgga gaatggttgg ggaaacatcg gcgaggatgc cgcgcggccg
     3541 aaggttgtaa tagcgatcac cgagtacaaa taacgacacg ggctgagtgg tgtgatatgt
     3601 atgaggttcg cctgtgacct cgttgatcat gcgctcacaa tttccgtgat cggcggtgat
     3661 aatcgccacg ccgcccttgt cagtgatcgc ttttaccaaa cgaccggcac attcatctac
     3721 agtctcaacc gctttaatgg ctgcggccag gacgccggtg tgcccgacca tatcggggtt
     3781 ggcgaagtta accaggatga aatcgtcgtc gtgcgttcgg acccgatcga tgacggcatc
     3841 ggccaatggc cgcgcgctca tctccggctg gagatcatag gtagccacct tgggagaggg
     3901 ttccaggtga cgctcctcac ccggaaaagg tgtttccacg ccgccgttga agaagtaagt
     3961 gacatgcgca tatttctccg tttcggccgc atggaactgc ttcaggccgg catcactgag
     4021 aacctccgcc aagggattgg tgatatccgt ctccggatat aggaccttga ccgggaaggt
     4081 ggagtcgtaa ttggtcatgg tcacaatatc gagatcggga atatgctcgt ggggaaaatt
     4141 ttcctccgcg ggcttgatga aggccgcgag gatctggcgc atccggtcgg cgcgaaagtt
     4201 aaagaagatg atcgcatcac ccggctcgac gagtacattt tgacgatcct cagggttcgc
     4261 agggtcgatg gccacaggca gaacaaattc atccgtgacg ccgtttttgt aggacgcttc
     4321 aagcgctttc gacagcgatg gcgcacttcg catcgtgccg tcatctccgc gatatccctc
     4381 gtgatagacg acatcctggt accccagctc cgtgcgttgc caccgcttgt cgcgatccat
     4441 gatgtagtaa cggcccgtca ctgaagcgat ggtgccgggg tgaccttcag ccagataggt
     4501 ttcaagcttg cgagcgtatc ccaatgcgct ttcgggggga gtgtcgcgcc catcggtgat
     4561 aacatggaga atcggcgcca ccccatgctg ctcggccgcc tgcaggagag cgtacatatg
     4621 cttgctgtag ctatgtacgc cgccttcgcc taataaaccg atcaggtgca actttccacc
     4681 acgcgatttg accccctcga gcgtctcgac aagcgtcgga ttggtataga aatctccgcg
     4741 gtcgatcgac aagttgattc gagtcaagtc ctgatagatg atccttcccg cgcccaaatt
     4801 gagatgcccg acctcggagt tgcccatttg cccgtcgggc agccctacgg cctcgccgga
     4861 agcatcgaca acagcgcgct ctccctcacg catccatcga tcatagttgg gcgtatgcgc
     4921 ctgcacgacg gcattcccat gttccatctc gcgaattccc cagccgtcca atatgatgag
     4981 agcgaccggt cgatagggtt tcattcgaat ttgctcctct gaacgacgcc aatcgttcaa
     5041 acgaaaaagc ggccgacctg tcggccgccg cttcttgtaa atattccagc tcctcaggtt
     5101 ggactcgaac caacaacctt gcggttaaca gccgcgtgct ctgccattga gctactgagg
     5161 aacaacagta ggtgattata ctaatagagc gttttgtgtc aagggaattt taatccctcc
     5221 ggctagtcgt cagccccttc gaacttgaac gaaatcatca cccgcgcgcg atattccacg
     5281 atctcgccat catcaagccg cacatcgagt ttggtgatct ccgccacacg cagatcgcga
     5341 agcgactcgc cggccgtttc gattgccacg cgcgcggcat cttcccagga agttttactc
     5401 gtcccgatta tctcgatcat tttgtagaca ctcatggcta tactcctttt ccgggccgat
     5461 cagggcctgg atgttatttt acgcgagttg cagaatcagc tcgcgagtga aaccgatcca
     5521 ccacatcatt ttagggccag gtattcgcgg ccgtgcagcc tgtctgaaat ccgcgaccag
     5581 atttgctcca gatgctcatc gtattgaaca tagtcgagat tatccgtatc gacggtcagg
     5641 acagggctaa ggcgaaagcc ggccgcccat tcatcgtaca gacgattcaa gtcctgcaga
     5701 tatgtgtcgg caatattgcg ctcgtactct ctccctcgcc cggcaattcg gcggcgcagg
     5761 gtagccgtcg atgcccgtaa atagatcacc aggtcgggcg gcttcaacag ttgggccaat
     5821 gtctgataaa gctcaaaata ggatgcccaa tcgcgctcgc tcagatgacc ttgcatatac
     5881 agattgcggg caaagatctc cgcgtcctcg tagacgctcc gatcctgaag cacagcgctg
     5941 ggctgttgga gcaagctgtg atgctgctgt aatctccggg ttagaaagaa aacctgggag
     6001 tgaaaactcc accggttcat atcatcataa aaatcagcca gatagggatt ttcggccaca
     6061 gcctcaaaaa ccggttccca acccagcttg cgagacagaa gcgtcacaag cgtcgatttt
     6121 ccaacgccga tatttccggc aatcgttacg aaataagtgg gcatgaatca gacctgtagc
     6181 gtgggaaagt tgacctccga ggtatatgac aaatcgtacg gcaaaacctg acatttgtca
     6241 ctattgcccg gccggggaag tagtagagta atagataatg aggcggctgc gaagattccc
     6301 gagcggccgt gatggggcca gttttgcagg cctcagagga gtgtagtcat gcgagttcta
     6361 atcgatcggg acctgtgcga cgcgagctta ccgttttgcc agcgctgctc ggcggcattc
     6421 atccgcaatc ccgaaggaat agatcgacac tgcatccgtg agatcatcga tgatggtcaa
     6481 gacctattga ccattcagat gttgaccgac ggccgtgaat tggagatcga actgtccgag
     6541 gaagaacgag agcttgcagc aatcgaagga tgggacgtca tcgctcagtt tgatccggcg
     6601 ctcttccgaa cgggagccga caaacgctgg cgcgaactga gaatgctttc gattcggtaa
     6661 gcagttcaac cgagcagcaa tttcgatatc atcgacgata gtcgagttat atacggctgc
     6721 tgatcacgcc tacccggctg tcacgagcag ccgatagatg atcacgctcc aaagtgccgc
     6781 gccgatcaat atagcgggca aaaagagagg atgtgccctc atgttggtct tgtgcctgaa
     6841 cgccaacata cccaacaacg cccccgcgaa cccgccggac agcgacataa ggtgaaaaag
     6901 tatttcaggg attcgaccac ccccggcctt cgctgaaatt ttgtcgtagg cgtagaaaac
     6961 aaaagtcacg acgccggcag caatgagcca gttccaatac cagggccacg ttgttgacaa
     7021 tgccagaacc aggaataacc cggcggccgc aacaatggct atgatcccga agattgtaaa
     7081 atagcgactc ctcattcccg gaaactcccg aacgaatggt aatctttctc tatcctctat
     7141 tcgatagaga ggatatggaa tttggcatgg gggactgttt cgtacgcgcc acaatggaac
     7201 ttgatcgtga acccattctc aagcgccgga tattcggcga tgcgggcgcg ggtttgagcc
     7261 atcgcataat ccaacagggc cataaagcga tcactgtgga tctcctgcat ctcggatgtg
     7321 ccgaagcccg attcaaacgg gacggcaagc caatggatcg ccgcctccgg ctccatactg
     7381 gtgataatgg cgaacttctg ctcatcgtca cgagcaatga cggttccctc tagctcgcca
     7441 cgcaaaatag cggcaaaagg gctgtcttca tactggtcat tctcaggcgt catgatttcc
     7501 cccacgttgc aataatcaag ctattgtaaa ttgaatctgc ctcgaactgc cttcagcttt
     7561 cggcgataac cggctcgcca tctaccccat aggcataggg catggctcgc gtgttaaatg
     7621 cccaacccac ctggccacgc gcatccaccg cgatcaggcc gccctgtcca cccgtccgtt
     7681 cttccagaac acgaatcgcc gattcgcagg ccgaacgcgc cgacaatcct gtaccgacaa
     7741 agtcgcaaac ccgtttacta atgactacgc gcattagggc ctcgccatat cctgtggcac
     7801 tcacggcggc cgtccaattg tcggcataag cgccgctgcc gactagcggg ctgtcaccca
     7861 cgcggccggc ccgtttgttg gctgttcctc cggtcgatgt ggcggcagcc agattaccgg
     7921 ccatatccag cgcgaccgcc ccgaccgtat cgccaatggc cgcttgatcg gcggattgtg
     7981 aggtgattat ttcatccaaa tggagattat gcaccaaaag ttcttcgacg ctgcaacggg
     8041 gaaaatcgat gctatcggcg aaagcattcg cgcccaggcc aaccaggaaa ttgtggccac
     8101 tttccgccat cacccgccgc gccaggctga tggggtgacg cacccgctgg atggcggcaa
     8161 tggcccccat gctcaggtcg cggccgtcca taatgagggc atccatttcg acatcgcctt
     8221 ccgcgttggg ataactgcca cgaccggcgt ccagaacagg gcaatcttcg agaacattaa
     8281 cggccgcctc cactgcatcc agggcagagc cgcctttgag aaggatagcc cgaccggcct
     8341 cagccgcttc ggcgcaagct gctttggcct gttccaaacg gttatcagag gcgctccagg
     8401 ccccggctcc accatgaaca ataatcgcaa cggtcatatt cgtgtgtctc cattccttgg
     8461 cgatgaacga attattagca ccaatcgggc gatttgtcga aatggcgcgc ccctcgatgc
     8521 acaacagcca aaaagcccgg cggagtttcc ctcgccgggc cttttggttc agtggcaaga
     8581 atccgtccct ccggattagt tgaggcgttc gaagataccg gccgcgccca tgccgccgcc
     8641 gatgcacatc gtcaccatgc cgaattggga attccggcga cgcatctcat gcatcagttg
     8701 gacggtcagc ttcgccccgg tgcatcccaa cggatgaccc agcgcgatcg caccaccgtt
     8761 gacattgacc cgtgattcat ccagtccgag gctccgaatg acagcgacgg attgcgaggc
     8821 gaaagcctca ttcaactcga tcaggtcaat atcggccagg cccatgccga cacgattcaa
     8881 caagcggggc acggccgcaa tcgggccaac ccccatgatt tccggacgca cgccgcccac
     8941 gttaaagccg acaaaacgca ggataggcgt caggcccagg gcctcggcct tgctgcgctc
     9001 catgacgatg accgccgcgg cgccatcgct caggggcgag gaattgccgg ccgtcaccga
     9061 tccgccttgc ttgaatacgg gcttgagttt ggccaaccct tccagcgtcg tgtccgggcg
     9121 aagatgctcg tcacggtcga agagcgtcgt catgcgcaca gggccatccg gcccggcgct
     9181 cacctcttca atcaccaggg gaacgatttc ttcccggaaa gcgccacgtt cataagccgc
     9241 ggccgccttg aggtggctat tgagggcgaa ctcatcctgt gcctggcggc tgacttcata
     9301 ttcgtcggcg acccgttcgg ccgtcaggcc catgcccata tagacctccg gcatgttctc
     9361 gaccatatac gggttggggt tcacccgaaa gcccgtcata ggcaccatgc tcatcgtttc
     9421 ggccccaccg gcaatgatca cgtccgcgcc gttggcgatg attcgctccg cggccatggc
     9481 aatagtctgc aagccgctgg aacaaaaacg gttcaccgtc tggcccggcg tttccaccgg
     9541 cagcccggcc cgcagggcga tggtacgggc gaagttaagc ccctgcgcac cctcaggcat
     9601 ggcgcagccg atgatgacat catcgatctc aatcggatca agttccggag ccttgcgcag
     9661 caggtcctga atgacggcgg ccgccatctc atctgagcgc cagttgcggg ttgtgccttt
     9721 cttcgatttt ccgacagccg tccgggaacc ggccacaata acagcttctc gcatcatcat
     9781 tatctcctta ttacccgttg tgccgcgacg tttggcccgc gatgatcgaa agacaacgtc
     9841 aggcggcaat ccgggtcggt gcgtcctagt tccgcagcgg cttgcccgtc tgcaacatat
     9901 acatcatgcg ttcgcgcgtt ttctcctcgc ccagcaagga caggaatgct tcacgctcca
     9961 ggtccagaat ataccagggg tcaacccagg ttgggccgct cagatcgcca ccggtcagca
    10021 cgtaggccag cttgcccgcg accttcgcgt catgctccga ggcatagccg ccccaatgca
    10081 aggagcgcac gcccagcttg agtgcataaa gagcgtcgcg accggccgcg cagatcttct
    10141 cgacttccgg cggcctgact ccaaattcga ccagttgcag tgcgcgctgc ttggccttcg
    10201 ccaggcggtg gtcgctgttc atgacgatgg tatcctgcgg ggtcaggtaa cccatcgcac
    10261 gggcctccca ggcgctcgtg ccaaccttgg ccagcgcaat ctgctcgaaa gcttcctgca
    10321 atggaggcaa tacatcagcg ttggcgattt tcatggtcgg attaaccttc cgtcgcagga
    10381 tctccttgca gccgccgccg gccgggatca aaccgacacc aaactccaca aggcccatgt
    10441 aggtctcatg atcggcgacc gcctcccaac cggccatcgc catctcgacg ccgccaccca
    10501 atgccatctg atggggtgcg gtgacgaccg gcttgggtgc atgccgcaga ttaaacgtgg
    10561 ccgtttgcaa ggccttgatc atcttgtcca gcgcatcgaa atggcccgac gccgcagcca
    10621 ccccgacagc gaacaggttc gccccggcgc agaaatgctc gccattgttg ccgatgacca
    10681 aagcatcaaa atcactatca agccgttcca gcgccttgaa agccatatcg ataatatcct
    10741 ggtcgatggc attcatcttg gcgtggaact cgaacaacgc gacaccgtcc cccatatcga
    10801 gcaggctggc gctgtcattg tgctcaaccg tcttgccggc ggcgcgcaag tcgctcacca
    10861 gaatgatatt tttgtccggt tcggccgcgc gataggcctt ggcgttgaag tcgtatacct
    10921 gagtagcgcc gttccgctta tagaaggact tatgccccgc ggccagcatc tctttcaccc
    10981 acggtgccac cctcaggcct tgagcctcca ttttttcggc ggtctcggcc acgcccagca
    11041 tatcccacat ctcaaacggc cccgcttcgt ggctgaatcc ccagcgcatg gcatcgtcga
    11101 cgtccgacag atgataggcg atttccggcg ccacataagc ggcataggca aaaccatagt
    11161 aaaggatatc acggacgtaa ttggcagcgc gatcatcgaa cgtcagtagc ttgcgcacgc
    11221 gctcgcccaa atcttcaaca ttgcgcaccg cgccgatgga gtcaaatcgc accttcggcg
    11281 tcggttcatg ggccattgtg gccgggttga gcgtccagaa ctcacggacg ccgttaactc
    11341 gctcctgctt gtagaaaccc tgtccggcct tgttgccaag ccatttcttc tcaagcatag
    11401 ccgtcatcag agccgatgtc ttttcggggc gcagcatctc gcgataggca tcatggggca
    11461 cagcgtcata gaggttgtta ttcacatgag ccattacatc gaggccaacc aggtccaaaa
    11521 gccggtaagt ggccgtcttc ggtcggccga tcgtcggacc ggtgatggca tccacctcag
    11581 caatggaata gccgttctgc agggcatgct ccagaccaaa tgacgaggcg acggcgaaga
    11641 accggttgcc gatgaagttc ggcgtatcct tgcaaaccac gacgcccttc cccaacacct
    11701 ccgaaccaaa ccgcttcatg aactcaacaa cttcaggatc agtgtcggtc gtggggatga
    11761 tctccagcag cttcaaatag cgcggggggt tgaagaaatg cgtgcccagg aaatgggcgc
    11821 gaaactcgtc cgagcgaccg gcggcgatct gcccgatcgg gatgccggaa gtgttgctgc
    11881 tgacgatagc gttgggcttg cggatcgcct ccaggcgttt cataagcgcc tgtttggggg
    11941 ccagctgctc gataatcact tcgattatcc agtcacaatc ggccagtttg tcgaagtcgt
    12001 cctccagatt gccgagggtg atcagatcgc cgcgatcggc gcgagccaga ttagccgggc
    12061 gggccttggt catgcgatca tagagggagt tgatgatctt gttgcgtgcg gccgggttat
    12121 tcttctcagc ctcactgaga tcaggcggga cgatatcgag caagacgacg ggaatgccaa
    12181 tatttgccag atgagctgcg atgccgccgc ccatcgttcc cgctccgata atacccacct
    12241 tatcaattcg ataggtcatt gaagcgctcc ttgttcagta gtgattcagg cacagaccaa
    12301 cgcagacata acgaatctcg ccaccggctt cgggcagcga aattcgttat atctgtttcg
    12361 atcttgtgcc gagtgcttat tttaattctc ggcgagagta tcccaaaata ctgcgggcga
    12421 cgattagctg gttaatttgc ttggttcctt cgtagatgtc gttaatcttg gcgtcacgca
    12481 tccacttctc gaccagccac tctcgcgaat agcccatcgg ccccagcagt tcaaccgctt
    12541 tctgggtaat ccaggtcacg gcatcgccgg cacgatactt gcacatactg gcttcgaggc
    12601 ggttgctttc cccgtgggcc atctgggccg tggcccgcaa cgtgaggagc cacgcgcctt
    12661 tatagcgagc ttccatctcg atgatgtcgc gctcgacggc cgtcatctca tgcagcgact
    12721 tggtgtaatc aatgacgatc ccttcttcga ccagcttgct ctttgtgaac tcaagcgccg
    12781 cacgcgcaat accaatcgcg ctggcggcca cagccgggcg cgatgcatcg aaagtcttca
    12841 tcgcgccgcg gaaacccttg cggccttcca tttcgcgcgc ctcttgatcg ccgagcatgt
    12901 tctcaaaggg aatccgggca ttgttgaaat gcaaagccac ggtgtcgctg gcgcggatgc
    12961 ccagcttgtg ctcctgcttg gcgatcgtga caccgggcgt attcccctcg acaacgaacg
    13021 atttgatgcc cgcccgccct gccttgggat caacggtagc ccacacgaca caaaggccgt
    13081 cggattcctt cagcgccagc gcgccgttgg tgatgaaaat cttttcgcca ttgaggatcc
    13141 actcattcgt ctcggggtcg aggacggccg tcgtgcgcat ggcgctgttg tctgagccgg
    13201 cacccggctc cgtgatcgcc attgcgcccc acttcgggtc gccttcggta aacttcttca
    13261 ggaaacgcac gcgctgctca ggcgtgccag ccgaatcgac ggccgcgccg cccagcatcg
    13321 ggtgcggcag gcaaaggtat tgcccggcat ctccccagct aagctgctcg atcatcatga
    13381 tgaacgacag catgccaaaa tcaggccctt cgcgtttggg cttggcgctg ccgttgacgt
    13441 cattactgcc gttagcgcca ttactgccat tattgccgtt gctgccgttc gagtgagcca
    13501 tgagcggctt tagtgaggtc ttgaccatct gctgggtgaa aggccaggtc atctggacaa
    13561 aagcctcagg gcgctggtgc tcgttctcgt ccaattgacg cgaatacggc cgcatcacct
    13621 gttcggcaac catacggatc atctgcaact gattttcaat gaatggaggt atctcaaaac
    13681 taatcatggt tcccgctcct cttgtgcgtt cgattcgcgc ctaaacaatc gccaacccgt
    13741 caaaggtggc aaacccgcgc gcattgcgca gccataactc aaccggatac tcgcgaatat
    13801 agccatagcc accgagaatt tgcacagccc ggtcggcgac ctgcatgacc atgcgatcga
    13861 cttgctgttt catgatcgtg gcgtcgggcg tggcgtcatg cccctgatcc agcttccagg
    13921 cggcttccca gacgagaagg cgagcctcat ctacttcagt cgccatatcc gccagcataa
    13981 aggcgattga ctgccgatgg gcgatcggct cgccgaactg gacgcgctct ttggcgtaat
    14041 cccgggcata ttcgaatccg gcgcgagcca tacccacggc tgcggctccc agcgccaccc
    14101 ggctgtgatt gaggatgagg ctgaaatcga taccattctc accgcccaat ttattcgccg
    14161 ccgggacgcg caccttgtct aaagtgacca gataagcggg caacgaatga atacccatga
    14221 gcttgttgcg gccgcccaca gtcaaccctt cggcgccggc cggcaccaaa aatgcctgcg
    14281 tcctgccgcc ttcggcggcg tagatcagga tatattccgc atcatttgcc tgcggcacaa
    14341 aggtcttcgt acccgacagc acataggcgt caccgtcgcg gacggcggtg gttcgcaatt
    14401 tatatgggct gaactggacg gccggctcgg tcaaggcggc cgtcatttta ggcggggttt
    14461 cctcgcaaaa ccggggcaga aattgggctt tctgctcatc cgtaccacag agtagaagcg
    14521 ggatcgccac caggttgggg gtcatcactt ccagtgtcac ggccaggtct ccccaggcaa
    14581 actcctcgaa agccagcgcg ccggtcacgg ccgagtaatc gccgaaacca ccgtattctt
    14641 ccggcaacgc agcaggcagg agaccgatct cccaaccggc cttgatgacc gcttcaggga
    14701 tatcaccctc ctcttcggca tcacgaaaaa ccttgcgcac acgctcccgg gaatagcggg
    14761 agatcgtttc cgcaagcatc ttctgctctt cgttaggtct aaaatccagc atggcaaact
    14821 cctcacaacc gagagattgt cttgtataac ttactgtcca gcgcggcgac ggacgatagc
    14881 atccgtcatc cgagccacgc gcacaatttc agcgacatca tgcacgcgca cgatatccgc
    14941 accacgatcg atactgatgg ccacagccgc ggccgtgcct tccatccgtt catccggagg
    15001 caaatccagg gtgtagccga tgaacgattt acgtgatggg ccactcaata gcgggtagcc
    15061 gagggccttt aattcaccga ggcgatccag cagctccagg ttttgttcca ccgttttgcc
    15121 gaaccctatc cccggatcaa tgatgatctg ctcgtgtcgt acccccgcag cgagagcgac
    15181 cgcaatctgt tccgtcagct cggccttgat atcgcccaga agatcgccat aggcaacccc
    15241 aacgtaccgg ccgccaaggc gggtttcctg agcgacatcc ttcggcttgc tgcggttatg
    15301 cataatgacg atcgggcacc cccggcgggc gatcaggccg gccatatcgg gatccattcg
    15361 tagcccccag acgtcgttga cccagttcgc tcccgcgtcg agagccaact cggccacatc
    15421 ggcacgatag gtatcggcag aaatgacgac atcgccgatt tcactacgta tcgcctggat
    15481 agcgggtata agccgcgaaa cttcctcgtc ggccgtcaca ggcttactgc ccggccgcgt
    15541 gctttccccg ccaatatcca gaatgtctgc cccctgctcc acaaaacggt gcgcctgttc
    15601 caccgcccgc tcgaccaatt tttcgccggt tccaacggca acaccatcac cggaaaagct
    15661 gtccggcgtc acattgatga tccccatcac ataggtacgt gtgccccatt gaaacatcac
    15721 tgtccttcga ttcttgcaat aagctatgcc ggcgaagcgt cagtcggcaa cgcttcgccc
    15781 ggaaacggca tctcaaacag gcgctcaacc ccagaagagt ccactttgtc cagcatctcc
    15841 tggacactta gcccggtttg cgggtggcga aagtcaggaa ttatgtcggc caatggaacc
    15901 aaaacaaacg agcgatcagc gataaaggga tgggggacgg ttagtgtttc gtcggagagg
    15961 accagatcgt catagaagag aatatcaata tctatcaaac gcggtcccca atggcgagtg
    16021 ggcgtgcgcc ccagctcccg ctctaccttc ttgatatcgg cgagtaattc gtggggtggc
    16081 aaagaagaga ttgcagcgac acagatattg agaaatgcgg gttgatcggt gtcgccccac
    16141 ggttcggtgg agtagacagg cgacaccgcg gtgacggtta caaattgacg caattcatcg
    16201 accgcatgct ggagattggc gtatcgatcg cccagattcg atccaagccc caaataaatt
    16261 ctgctcatat gatttcaccg ccgtgccaaa ggtcgtagct gttaatggtg cccaatatga
    16321 cgcgctgcat tattatgcat gtttataaca cactgcgtca tgagtgtcaa cggggtaata
    16381 tcttcgggaa tgacagattt ctcgaattcc caatcgcagg cggctagaat agcttcatgc
    16441 gaactgtcat agcattgcta attcttgcaa tgaccgctct gggggcgaat aacgtgtcgg
    16501 ttgaggcaca acaacccctg ccccatgtcg ttgatccggg tgacacgtgg ccggcgctgg
    16561 caatgcgatt tggcgtagaa gagagtgaac tgagagccat gaaccctcag ttcaacacgc
    16621 tgcgccagcc ggcaatcggt tcgacgatca gcctgccgga agaggcaagc gaacagccag
    16681 gcaggcttgt tcgcgacggc gacgccggca tcctcgccat agctcttcaa tacaatgtat
    16741 cgccgtggtc tttggctctc caaaacgagc ttgattcacc atatcgccca acgttttatc
    16801 ggccgctgtt tgtcccgaca gagggcacga ttcgtgatct gccgcaaggg atcgactcac
    16861 tggaagtatc catgtcccct gctctaccgg gggtcgccct ggggatacgg gggacatcac
    16921 agcaagagac gataaacctt aacgggcacc tgggcgggct tccccttgca ttcgccgccg
    16981 agaacaaccg ctttatcggc gtagcaggaa caggcgcatt cttcacgggt ggagagccgg
    17041 agcttgtgct caaatccggt gatgccccgg cctgggttca accctggcaa ttcgcgaagc
    17101 gtgaatggat ctatcaggag ttgaccctta ccggcgaggc ggcgcagatc gaccaaaatg
    17161 cgcgtgatga agaacgggcg cgattgcgcg aattatgggg ccaaataaca ccggagccat
    17221 tatggcaagg cccgttcgtt acgccggtca cgacttatct ggaaatctcg gccgaatacg
    17281 gggcgcggcg ctcctataac ggcgggccat atctcaccta tcatgagggc gtcgattaca
    17341 gcgcctatgg cggcacaccg gtcatggctc cggccgcggg caaggtggtc ctggccgaac
    17401 cgctttacgt gcgcggcggg acggtcatca tcgatcatgg attaggtatc ttcacaggtt
    17461 actatcacct gtcggccatc catgccatcg ccgggcaggc ggttgagccg ggcgatctcc
    17521 tgggcgaagt tggcaccacc ggtctctcga ccggcaatca tctccactgg gatctgctga
    17581 ttaatggtat ctgggttgat gcagccgcct ggcaggaaca acggatggac tgctggctcc
    17641 tcgagggatt ggggcgatct tgcgccacag aacagccggc agattgagtg gttactcttc
    17701 cgtaacggat cgatcgccga aatcaaacag ctcgccgatt gagcggcgga agtagttctg
    17761 gcgaatcgca ccggcaaata ttggcccggt ggataacacc accagcctgg actggtcgga
    17821 agagttttgc ggcaccgtgt ccgtcgtgac gatctccctg atttccggca cagcctccaa
    17881 tcgcgccaaa gcgccatgga gaaacacacc gtgggtgcaa acaacactaa tctcttcaag
    17941 cccatgatcc agaagaagcc gcgccaactc gacgatagtc ccgccggtgg cgatttcgtc
    18001 atcatggatg atcgctcgct tgaagcccga cacctggcgg ccgatcaggc cactatattg
    18061 tacgcgggtg tcgtcgatgc gcgtcttgtt cgccgtcgcc acaccaagtc ccagcatatc
    18121 ggcaaaacga accgacgatt tggcactgcc tacatcaggg gaaacgacta tcgtatcctc
    18181 tgcgctatat tggcgcgctc taaagtaatc ggcaaacaag ccgcgagcag tgagcggatc
    18241 agccggcaca ctaaaaaaac catgaacctg cggcgaatgc agtgtcatgg tcataatgtg
    18301 agtcgccccg gccgtattga gcagatccgc gacaagacga gccgtgatcg agatgcgagg
    18361 ggcgtctttt ttatccgacc gcgcatagga gaaatatggg atgacggcat ggatctcccg
    18421 cgcagcggca cttttggcga tgtcgagcat cataagcaac tcgacaagat ggtcgctgac
    18481 cggcgggaca agcgattgca caatgtagac gacgcggccg cggacgctcg cccccaattg
    18541 cacgaacagg ttatcgttgc tgaaccgcga aacgcgagtt tcctccagac gcacgccaag
    18601 attatcggcg atagatttgg cgagaccggg attggaacgg ccggcaaaca cacgaacatc
    18661 actctggctc aaggactgga ctatacggct catatttttc cctccgggga ggaacacagg
    18721 ctatacagga tcttcgccta tctattatct tcgccttcca gaagcaatca aggacgagtt
    18781 agaagattac aaggttacct gtgcccttaa aaccgcgcat caagggccat cgcctgcttg
    18841 agcaaataac ggtagcgcgc ctgtgacacc tcgacagacc ccaggccggc taaatgagct
    18901 gtctggaact ggacatccag aagagtaaat ccacgtccaa gcagatgttc aacgagataa
    18961 tataaagcga ccttgctcgc gttcgaccga atactgaaca tgctttcgcc ggcgaaaagg
    19021 ccgcgcaggc tgaccccata gaggccgcca accagctcgt cacccagcca ggcttcgacg
    19081 ctgtgggcaa aacccaattc atgaagccag gtgtaggcct cgatcagttc atcgctgatc
    19141 caggtcagtt cgcgatccgg ggccggagcc gcgcaagcgg ccatcacctc cgtgaaagct
    19201 gaatcaaaac gcacatcgta tctgccggaa cgtatgacac gccggagaga atgggacaca
    19261 tgcaacctgt ccagcggcaa tatcgcgcgg ggatccggcg aataccagga tatctgccct
    19321 tcctcgtctg ccatcgggaa gatcccttgg gcataagccg taagcagcaa ttcaggcgtc
    19381 aagtccatgg taagttcatc gcaagtcacc tcataattgg ggatcgcaat cagggataac
    19441 tttcccttca tcgacaatgt tttaattccc cgatcttgtg cctataattc tccctaacct
    19501 caccctatgg aacaagtgta acatgtatga tgtcgaatca ctccgccgcg aggaattccc
    19561 cgctacggct actctaactt atttgaatca cgccgggatc tcacccttac cgcagcggac
    19621 caagcgcgcc gttcaaacgg ccgtcgagag actgagcgag gagccgagcg cgtttttcgg
    19681 aaaatatgct cagcccgcat tcgcgacgct ccaggaatca ttggcacgat atatcaacgc
    19741 agacctgccc tcggagatcg tgtactcaac ctcgaccagc gcggccctca atggcgttgc
    19801 acaggcgatt gattggcagc cgggcgacaa catcctgttc tgcgatcagg agttcccttc
    19861 caatgcctac ccatggatga gcctggctcg cgatggtgtc gagagccggc gcgtcccctc
    19921 ggagggaggc ggcctgaccc tgcgtcaggt cgaagcgcac gcagacgatc gcactcgcgc
    19981 cgtcgccgtt agcgcaatcc agttcttctc cggccatcgc acagacctgg tcgcgatcgg
    20041 gcgtttctgc cacgaacaca atattctttt catcgtcgac gctattcagg ccatcggcca
    20101 catggtgatt gacgtgcagg cgatgaacat cgatgtcctg gctaccgggg gcatgaagtc
    20161 gcttctcgcc ttgcccggcg ccggattcat ctatgtgcgg gatgaggttt cagcgaagat
    20221 gaaccctcgc cttatccacg gcaactccac cgtcgattac atccactggc tggcttacga
    20281 tctgactccg cagccggggg cggcgcggct atcgagcgga acgaccaacg tgcccggcat
    20341 actctcaatc ggcgcgagtc tgaccttgct caacgaactg aatccggccg acatcgacgc
    20401 ccacacgacg gcactaatcc attatgcgat agacggcctg acccgcgatg gatacgaggt
    20461 catcacgccc aaaagcgcat cagggccaat cgccaccttc cgttccccat tcgacagcga
    20521 aacgaccgac cggcttgtgc aattcctgtc ggacagacag ataatcgtgt gcaagcatct
    20581 ggacgccggc acgtcggctt acgtgcgcgc ctcggtccat tgctataaca ccgcggccga
    20641 catcgaccgc ttgctggagg ggttacgcgg cttcgtcaat tgaaatcgac gcggggttcg
    20701 tggtcccggc tgctttccca ccactgacgg attcggccgc cgatcgggcg gccgatcatc
    20761 ctctccagat catagacgac atcccacaaa cgggcgaagt ctacacctgt ccccaggccg
    20821 accttgaacg ccagaaaaac caggtcctca gtggccagat taccggccgc gccgggcgca
    20881 aaggggcatc cgcccaaccc accaacgctg gagtcaaagg tgcggatgcc cgcctgcaag
    20941 gccgtcgtgg cgttagccag gccaagcgcc tgcgtatcgt gcaaatgaac agccaggcgc
    21001 tcgatggcga cgcgtcgggc gatcctgtcg atcaggcggc cgactgatag cggatcggca
    21061 tgacctatgg tgtcggcgat ggcgatctcg tccacgcccc aatcagcgat ctcctgcacg
    21121 gtcgccatcg tcttcagcgg agatatcacc ccctcgaatg ggcagaccca ggcggtcatc
    21181 acataaacgc gggtggccac gccgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnntttt gcaacccatc ccgcggcccg acctcgtaga ccttcagcat ctcggtaacc
    21361 gaatagaccg catcaacttt ccagcgtcga agtatcgccc tcatcctggc ccaaggcacg
    21421 agctttcaac acgcgccgca tgatcttgcc cgaccgcgtc ttcggtagtg aatcgacgat
    21481 ctctaccgat gatggtttgg caatcgggcc catttcgtga cccacatggt cgatcaaggc
    21541 acgggttagc cgttccgaag gctcgtagcc caggcgcagg atgcagtacg ccttgatgat
    21601 atggccacgg agctggtcgg gcacgccaat cacagccgcc tcggccacag ccggatggct
    21661 gactagcgcg ctctcgattt cggccgttcc cagccggtag ccggacacct tgatcacatc
    21721 gtcgatgcgg ccgataaccc agatatagcc atcctcatcc ttccgtgcac tatcgcccgg
    21781 caaataccag ccctgctcat catatcggct ccagtattgc tgcacatatc gatcaggatc
    21841 accgaaaatc gtgcgcaaca tggccggcca tggcgtctgg atgaccagat atccttcctc
    21901 gttaggagcg gccgattcgc cttcttcatt gaccacgtca accttgatcc ccggaaaagg
    21961 tttggccccc gaacccggtt ttagcggcat tgtcggaagc ggcgtgatca tgaacatggc
    22021 cgtttccgtc tgccaccagg tgtccatgat ggggcagcgc tccttgccga tcacgcgata
    22081 ataccagcgc cacgcttcgg gattgatcgg ctcgccgacc gatcccagca agcgcaacga
    22141 agacagatcg tggaggcccg gccagctctc gccaaaacgc atcagcccgc ggatagccgt
    22201 cggcgcggtg tagagaatcg taatgccgta cttctcgacc atcgcccacc agcgatccgg
    22261 gttaggatag gtgggagcgc cttcgtagat gaagctcgtg gcgcccagga caagcggcgc
    22321 atagacgata tagctgtggc cggtaatcca gcccggatcg gctgcgcacc agtagatatc
    22381 gtggggcttc acatcaaaga cccatttgag cgtgatcgcc gtcccgacca tatagccgcc
    22441 atgcgtgtgc aaaatcgcct tggggcgacc cgtcgtgccg gaagtgtaga ggatgaacaa
    22501 cggatcctcg gcatccatat gctccgtggg agttttgggg tcggcaatag gcagggccat
    22561 caggtcgtga taccaatggt cgcgttcggc cttcatctcc acttcccggc cggttcgctt
    22621 gacgacgatc acgctctgga tagggtttgc cttctccagt gattcattga cgatcttctt
    22681 aagctccacc acctcgccac gcagccatgc cccgtcacac gtcaccagca ccttcgaatt
    22741 actatcaacg atgcgatcat aaagcgcctc ggtcgagaag ccgccataaa cgacggaatg
    22801 gatagcgcca atcttggcgc aggccagcat ggcaacaacc atctccggta tgcgtcccat
    22861 gtagatggtc actcgatcgc cacgccccac gccctgagaa tggagaacgc cggcaaactt
    22921 cgatacctca tggcttagct gctgataggt ataggtgcgg ctgtcccccg cttcgccctc
    22981 aaagataatg gcggccttgt tgcgcaccga cgtcctcaga tgtcgatcca gagcattatg
    23041 gacgatattg acttgcgcgc cgtcatacca cttgtaaaaa ggcgcgttgc tgtcgtcgag
    23101 aaccgtattc cagggcttgt accattcgaa attttcggcc gcaattcgcc cccagaatgc
    23161 cgctggatcg gccgctgcct cagcataaac cttgtcgtag tccttaatgt gggcatgggc
    23221 actaacttcc ggcgcaggat agtaaacctc tccggtcagt cctttctccg aagacatgat
    23281 tcctccgtga cttgatggat aaagagtgtg tgatgttaag caactcttgg ctaaccaagt
    23341 ataatggtca tattcggttt cgtaaaattg acgtggatgc tatgagtaaa cgcaaaatcg
    23401 taatcattgg cgcggggagc gccagctttg ggccaaccac attggccact atcgtccgca
    23461 atgaggcgtt gcgcggcagc gaactggctc tggtcgatct ggatagaact gcggccgaga
    23521 ctgcggcatg ggtagctcgt cgaatgaatg agtcatggca agcggacatg accatttcct
    23581 ccacaacgga tcgtcgtgaa gcgctgcccg gcgcaactca cgtcgtcgtc tccatcgagg
    23641 tcgccccgcg cgaggagcat tggcggctcg attgggagat tcccttgcgg catgggttgc
    23701 gccaacccta cgccgaaaac ggcggacccg gcggtatgat gcacaccttc cgccaggtgc
    23761 cggcccacat ggccattgca cgagacatgg aagttctttg ccccgatgcg tggctgattc
    23821 tttttagcaa ccctttaccg cgcctctgcc gggcgatctc caagtacacg cgcgtcaagg
    23881 tcgtgggaaa atgccaccag atcaatatcg gctacgcgct ggcggccgcg ttactgcatc
    23941 gtgattataa tatcccggtg ccggaaggtg tggtgctgca ttccgacccc ggcaacgccc
    24001 ccaccatata cacgctatcc catgcaggcc gcaagcattt cgaaatcacc tcggccggac
    24061 taaatcactt catttggttg ctcgatatcc gcgaccgccg gacaggcgag gacctctatc
    24121 cgcggctgag agaagcgatc gatctcgcgc cccccacgct ggaaccgctt gccctggaac
    24181 tgttccgcac cttcgggctg ctgccgatcg ccggggacac gcatatggcc gaatatctgc
    24241 cgtggctcca cgacccggtt gccggacctt gggaaaccta tcagctgccg ctctatgact
    24301 ggaccggcaa cgagagcgtg cgcgaagcac tccattcact gatggcagcg atggggtcgg
    24361 gggatattcc tgtggatggt atgcgggaag ccgttagcga aggtgcgacc gaattggtcg
    24421 aggcgcttgg tgacggcgac agctacgttg atgagacggt caacgtgccg aatcgaggcg
    24481 caattgccgg attgcctcat gagacaatcg ttgaagtgcc ggcttatatc agtcataccg
    24541 gtatccgtcc gttgcaaata ggagcgctgc cctaccctgt cactgagcta tgtcggcggg
    24601 aggctgccct ggtggagctt gttgttgacg cagcggtgac cggcgatcgc tctctggcct
    24661 tgcaagcgct gctgctcgac ccgatgatca ccgatatcgg ttccgcgcgc gcaattctcg
    24721 acgactatct gacgaccttc gcggattatt tgccgcaatt tcaataaaca gggctaaatc
    24781 ctgagcgcgg gaacgcccat ctacgatcga tcgtccatct catcgaacga ttggacaggt
    24841 gcaactgcct cctcttccgc aaaggacatg ggggcaaccg catcatcatc ggctgtctca
    24901 tcgggaaaca gatagccgca atagggacaa atatcccaat gaagctccag cggcgattgg
    24961 cataccgggc atggctggcg caagtgagtg tggcaggttg ggcacaggaa ccagtttgcc
    25021 tgcgtcggcc gcgaacaggt gggacatacg ggacgctcct cgatctgttg taacagcgct
    25081 tcctgttcca gcgcccgatc ataggcagcg ctcagggttt cccgcggccg aagcagccaa
    25141 tagacgacga gtccaacaat tggcacaagc gcaatcaaca agacggtgaa gaaaaccgcc
    25201 cacccggatc gggaacgggc acgaatgtcg cgaaatgccc aaatgacaac cgccagccac
    25261 aacagaagca acactccacc cgaaatgccg agggcaggaa gcagataggg ttggattaga
    25321 tcgtaccaaa tcataggcgc accattttga tgtatagtag cacaaggcag taaaacaaaa
    25381 agcccctggg cagaaacccg ggggctttta ttttttatgg ggtatcgatc tgggatcaaa
    25441 caccttgctg ctgttctttc tgcgatttgc gcgtacggcg cgacgccttc ttcttggcga
    25501 tgcggttcag ttcgctcttg gatacgaacc agcgcttccg gcgcacgtca gtcagcaaac
    25561 ggctcttgac aacctcacgg ctgaaacggc gaagcaattg ctcctgcgat tcatcagatc
    25621 gtagcgttac cttaggcaga gtattcaccc ccttcttttt gtagttttga catctc
//
LOCUS       DAOMQG010000013        24897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_863_length_24897_cov_10.433097, whole genome shotgun sequence.
ACCESSION   DAOMQG010000013 DAOMQG010000000
VERSION     DAOMQG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 24897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24897
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_24897_cov_10.433097"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLC06_01775"
     CDS             <1..467
                     /locus_tag="PLC06_01775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRO89202.1"
                     /translation="EAVVAAEGAAKDVEHKAREDARGIIATAQSEATEERNRILADLR
                     GQVAAISIAAANKLVGDSLDEKRQHELINDFFSRVPAGVSELKGEAAEVTSALPLTDA
                     EKQSAAKVLGIKDVHFKVDPSILGGLVVRVGDRVVDDSVSNRMSALQESLRH"
     gene            723..1337
                     /locus_tag="PLC06_01780"
     CDS             723..1337
                     /locus_tag="PLC06_01780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRO89203.1"
                     /translation="MSQEQPVPQRATLTDQLRARTKFIIDPVVDVLARFKLGPDFLTV
                     LGFLTHFLFAWLIAMGEFRWAAVAIALISPLDALDGALARKLGRKQGGFGAFLDSSLD
                     RLAEIVLFGGFIYYYYSNVNPLMLAVAYLAVTGSLMVSYARSRAEALGFQAKMGLLSR
                     VERYVVLIVFLALRMPHIALGILAAFTYFTFFQRMWAVWKQSRD"
     gene            1352..2533
                     /gene="lgt"
                     /locus_tag="PLC06_01785"
     CDS             1352..2533
                     /gene="lgt"
                     /locus_tag="PLC06_01785"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRO89204.1"
                     /translation="MSRRIDPWWYAIALVLLLAGGLYAYHLATGLTPPRAAVTILGFP
                     VYWYGIWIVTGVALGAWLVAGLAAERARRIFETTVPVDVRARPLAEAGLPDEVVAVLA
                     SRNIGALGPLLMDVGLDPRRLGLKKAQTTEMVTSLAAAPDIDPAWVSDAPWRRWNPEH
                     VWNALIWILILGLIGARLYHVLTPSPSMAALGIYSPLDYFRDPIQLINFRGGGLGIFG
                     GLIGGLLGLVIYTRRARIPFLGWADLAVVGLALGQAVGRWGNFFNQELYGRPTNLPWA
                     VHIDPIYRLPDYVSFERFHPAFLYESLWSLATFFVLYRLTRRYSERLLPGEMMGLYLI
                     AYAVGRSLLELVRLDSRVIPFFGVETGLAVATAVSIVIALASAVMLIGRRWSRRRRGV
                     G"
     gene            2586..>3355
                     /locus_tag="PLC06_01790"
     CDS             2586..>3355
                     /locus_tag="PLC06_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89205.1"
                     /translation="MSTKRYNPVLVLVIAALVVLLAVLLGGRLLNGDDSLLRNVAMGD
                     SAITPNADGTTDATPIRYELSRNAAVSIYFEDAAGDRFYFRQDKARGAGEYEVLFSGV
                     VDGYRLAGDEIEGEILSRLLHDGEYTWTIEATDFDGITERHQGTITISGADPLLPELR
                     NFTLDRHLFTPNRDGIDDRVLMQYYLPKEVISARVYLRRPDGSELPIAEQERDIPANM
                     PGRHYYDYEGGVDHDETPPPDGTYTVVAEAQDAEGQRVR"
     assembly_gap    3356..3455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3456..3761
                     /locus_tag="PLC06_01795"
     CDS             <3456..3761
                     /locus_tag="PLC06_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89206.1"
                     /translation="ENELTNYPYRWALGKGSDLEEIDGHLYLMPGKRAVITGGIRVVD
                     VFGNRNPQPIWAGLIHEDVQITEFNNRVDPQSILVDIPNAAARQDCEPREIPVRVAD"
     gene            4065..5270
                     /locus_tag="PLC06_01800"
     CDS             4065..5270
                     /locus_tag="PLC06_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HRO89207.1"
                     /translation="MGIVQLGLLLVACNSPVAQPPLAGSPAIEVTAPAVTPSTAVPVP
                     VAARTLAATPAPQIHEVTPAVTMIPVTVEVTRPPVGTAERPVQLLFPPVAAGAVIAQR
                     AAPLVAALQAATGAEFVVGVVDSEAALVELLCTAPEDVIGFISAAAYTIAHDQCGAQP
                     GLVAVREDGLTWQMGMLAIRPGGAAGLADLAGKRWAVADTHSLPNYLYFLSQMTAAGI
                     APGEIVDAPEESSALLSLYNGEVDFSTASFIPPIMPHDRQWIAGETEAEEWRLLGIPP
                     SRSPIGYVVVAGEPEFGGYRLRDARARMFDTTPEIFNATRILALSEPIPNDTIVFGAN
                     FPLGIARQALATMTEFAASDACATSLCSADLFGWAGLQPAEDAAYDPIRVIKDTLELE
                     AADLFAELD"
     gene            5272..6195
                     /locus_tag="PLC06_01805"
     CDS             5272..6195
                     /locus_tag="PLC06_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89208.1"
                     /translation="MIDVAIIIVSWNVRNYLSDCLRSVAADLRVSRLQGEIWVVDNAS
                     TDGTVALLEDLFPQVHVIANQTNAGFGAANNQGMRAAAAHHPRYYFFLNPDTLVRPGA
                     IGRLVKCLDERPKGGMAGARLTYGDGRFQHSAFSFPGLGQLVFDLYQMPSRLYESRLN
                     GRYPRRYYRTDRAPFTVDHPLGASMLVRADVAESTQGFDESFHMYCEEIDWSWRVRES
                     GWEIYAVPAAEIVHFSGESTRQIPAQSMVNLWESRARLYHKHHGRIRQALARWLVRSR
                     MAARARHAGDPELKAAYEHIVDVWSMNGKVG"
     gene            complement(6280..7233)
                     /locus_tag="PLC06_01810"
     CDS             complement(6280..7233)
                     /locus_tag="PLC06_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRO89209.1"
                     /translation="MTQRKPALLALIILLLSAVACRNAGSSTTLLPVATAPGDAATLP
                     PAAAATNTAVPPSPTPTEPLAALVNGEQIFLSDFEAELARYSTQQTTPGSETTDVREQ
                     VLNALIERQLIMQAAATAGVVATPEMVDQRLAELRAADEAAFQTFLQTNGWTEEEFRE
                     TLAAEIVTGEMIARVTADVPTTMEHIRASYIQMDDGALAQSVLERARAGDDFAFLAEQ
                     NSVDRVTGANGGDLGFFRVGSLLVPDVEAAAFALRPGEISDVIAVTDAAGKTTYYIIK
                     VTERDPNRELTADDRYGLMQAAYDVWLEGLWANATIERFIQ"
     gene            complement(7264..8526)
                     /gene="hutI"
                     /locus_tag="PLC06_01815"
     CDS             complement(7264..8526)
                     /gene="hutI"
                     /locus_tag="PLC06_01815"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRO89210.1"
                     /translation="MSEPVDLLIHSAEQLCVVPTHDEGPQRGAALGDLGLIPDGALVA
                     RNGRVVAVGPSAELRARFQAEITIDAGGRCVIPGFVDPHTHLPWAGDRAGEFEQRLAG
                     ATYMEIMNAGGGIMSTVRNTRQAGIDELVADNLPRLARMLRHGTTSAEAKTGYGLDTA
                     TEIKQLDAIVALAMTQPVELTPTFLPAHAVPAEYRGDTEGYVRLVIDEMLPAGAAWMH
                     ERGISLFCDVFCEDGAFDLEQTRRILTAAAALGYRLKVHADEFVGLGGTKLAVELGAT
                     SADHLVMTPEADIIALGRGDTLAVGLPGTPFGLAHKEYTPARAILAAGGALALATDLN
                     PGTTWCESMQMVVALACRYMGLTQAQAIVAATLNAAFAIGRGHDVGSLAPGYQADILI
                     LNVADYRQVGYRYGTNLVETVIKRGRIV"
     gene            complement(8519..9736)
                     /locus_tag="PLC06_01820"
     CDS             complement(8519..9736)
                     /locus_tag="PLC06_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO89211.1"
                     /translation="MAQPQESASAVATWSWDGRRLAQLGVLTFARFLQNTLLRVVYPF
                     APAFARGLGVPIETIYFIVSLRNLLGLFAPLFAPLPERYGRRVVMSTSLLIFGIASLL
                     VVGVPVIWTFGIAIIMAGVLKVIYDPAMQSYLGDIVPYHQRGKALSVTEFAWSGAFLV
                     GAPLTGWLIANQGWPAPFLWLGVGGLAAAAMLWRALPPAGKGRRHGIGISYTWQMLRK
                     HPIIWAAALYMMLVLVAQETLFIVYGDWMESTFNLSLTSLGFATTLIGLAEMGGEATA
                     GWSVDRFGKRPVVILCGILNAIIFLIIPFSTGSLATAMAAMIALFFTFEIAVVGAIPL
                     LTELVPSERAAVMSMSLGAMAAGRTIGSLVGPYAWEVAGVAGNSTISAIMMLLAVLVL
                     ARWIREGKEASHE"
     gene            10027..10347
                     /locus_tag="PLC06_01825"
     CDS             10027..10347
                     /locus_tag="PLC06_01825"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRO89212.1"
                     /translation="MSVAELFNSMPGRLQVDKAAGVSMSILFDLSGDGGGQYLVDLKD
                     GKLNVAEGAPAAAPSATVKMAADDFKAMSAGSLNPMMAFMTGKIKVDGDLNSVMKFQS
                     LVGF"
     gene            complement(10418..12241)
                     /locus_tag="PLC06_01830"
     CDS             complement(10418..12241)
                     /locus_tag="PLC06_01830"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HRO89213.1"
                     /translation="MNDQERHNRTVMLRRVKTVWLDGVLEESLHGGEIIDLGFAYRPA
                     ALGNSGVPSWQQAPEYDYLLPVGTRIEDVFAAADRVLLVLGEPGAGKTTMLLQLVSHL
                     MAQAEADETCPMPAVFSLATYTNGRTLSQWLVEQLAGNYEVPRKLGEQWIDAGQFIPL
                     LDGLDEVDPAHRPACAEAINEFRARHPGVPLLVTARNRDYQALATRLHMDKAIILQPL
                     TLEQIDTYLQRRGNKLDGLRASLAADHTLQELAQTPLMLSIMTLAANRPPESGAADLG
                     DGLLSRQHLFDVYVERMARYRGKNMSYSPSDTISWLSWLAKQQTRAMKPTFFLEDMQP
                     SWLPDDAGRGFVRQMRLSVFGILAGAAVPGALLLLLYNRWGAAGALLLVGLFAAAVPA
                     ITGRFLVRGRLPFKRIETVESLGWSWPWAALGFGTGAAVGLGLGALVAWLDRWTSTPW
                     PALLSVAGGVLTMVENALLRSDLRLRTTPGQGVEQSRRNGLMVAGLVIATTLILGAVA
                     VAVAAAVTSRQAFIPAAAWLLWLALFLGAGCGLSFGFLAWLQHWRLRSILHDRGDFPD
                     DATAFLNLAAERNLLRKVGGGYTFVHALLLDYFSRRGEQAR"
     gene            12368..13231
                     /locus_tag="PLC06_01835"
     CDS             12368..13231
                     /locus_tag="PLC06_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO89214.1"
                     /translation="MNDEFARALWRIYNRPDSPALWQGGGNLPWNDPDFSERMLREHL
                     DEGHGAATRQAAERAAQLDWFQEQLGLRPGSRVLDLTCGPGLYAVALAERGMNVTGVD
                     FSPASIAYARELAVAAGVGDRCRFIETDVRDYEPEREGYDAALFLYGQLAVFPREEAA
                     ALLGKVASALRPGGWLVVELLDPARVDKSDSSWWFTDDTGLWGGRPFLHLGERRWNAA
                     ERASVERYTILHLESGVMDEIILCDQTYEAEEMAGMMRAAGFNEMKSFAAWAGLPLYD
                     AEEWVAYIAQR"
     gene            complement(13233..13727)
                     /locus_tag="PLC06_01840"
     CDS             complement(13233..13727)
                     /locus_tag="PLC06_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89215.1"
                     /translation="MNVDYLVLAARIPQELAELNQVISRAERGNIAAKQHAEDSDLYI
                     DAVALNLHDFYTGLERIFRQIVTTVDRSMPASEAWHRDLLRQMCVELPGIRPRVLTEV
                     TCTALDDFLRFRHVVRNVYAFQLDGDRVGHLTLEARTVFDQLYSELMMFVIFLEQSGQ
                     DTTS"
     gene            complement(13724..14101)
                     /locus_tag="PLC06_01845"
     CDS             complement(13724..14101)
                     /locus_tag="PLC06_01845"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRO89216.1"
                     /translation="MAESIWSQEELAVFRANAVYLRVKDQEMEKRRRETAWAAAHAAA
                     DLLRQDFHASRVVAFGSLVRDGSFTRWSDVDIAAWGIDSKDTLRAIGAMMDMEMGIDV
                     NLVDVATATPALMESIMSEGVEL"
     gene            14176..14874
                     /gene="ccmA"
                     /locus_tag="PLC06_01850"
     CDS             14176..14874
                     /gene="ccmA"
                     /locus_tag="PLC06_01850"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HRO89217.1"
                     /translation="MIRLNGVVKQYGLNPVLRGVDLNVEQGEFVTLVGPNGAGKSTLM
                     GIVATLLRPTSGDVFVGGWRLPEQADRVRRHIGLVSHHSLLYRDLTAAENLAFFAKLY
                     RMEDADERVADALHKVGLYARQRDPVGTFSRGMVQRLTIARATLHEPDVLLLDEPYTG
                     LDQEASYLLDELLRGETARGRTILMITHDLGHGLGLADRLAILYGGIIAREVSRAEIS
                     PAAVYDLYAEVTMA"
     gene            14950..15651
                     /locus_tag="PLC06_01855"
     CDS             14950..15651
                     /locus_tag="PLC06_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRO89218.1"
                     /translation="MKEFWAAVWAVVWKDLRIEGRTRQTVSVMVMFSVATIIMFNFAL
                     GMAVNVNLSAGRDVAPALLWAIVLLAAILGLNRSLSLDRENQVFDAMLIAPIPRTALY
                     AGKVISITLFTLMLDAILVVLFTVFFNQPYYLPMIMLVLALGTIGYIAAGVLITSMTI
                     QSRTREVLLPVLLLPVSLPLVLPAAMATTTYIASAMRGEASWSLIQSPMLLVVAYDLM
                     MLGVGFLTYHFVVES"
     gene            15781..16767
                     /gene="ccsA"
                     /locus_tag="PLC06_01860"
     CDS             15781..16767
                     /gene="ccsA"
                     /locus_tag="PLC06_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRO89219.1"
                     /translation="MSPVSLFNPRLDRWIRIFNVAAVVAMVIAIGAAFFYAPVEMTMG
                     NVQRLFYFHVGAAWVGSVVFGVALLCGVLYLRGGRRVYDVISLASVEVGLVFLTMTIV
                     GGSFWGKPAWNTWWIWSPRLTLITVAWLVYAAYFMLRGAIEDPHKRARYAAVYAIVAF
                     VTIILTFVSIRIFRDIHPVVIGGTTEAAAGAAEGLQQFNGLDSAKMGITLMISVIAFS
                     ILGTAWILVRMRLQDMIDYTDNLKTRVAAYLSSRPRRSPSPVSSLLLTLPALQPRIAD
                     PNGFNQYLIGGYVIMSGLALAYMLYLYVRQRNLQKDINLLSQLLQEDKRPRR"
     gene            16861..17976
                     /locus_tag="PLC06_01865"
     CDS             16861..17976
                     /locus_tag="PLC06_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HRO89220.1"
                     /translation="MSDLKAPAPQTRKLSTTQLVLIGLAVLLVLFLILGSLIRPDTSN
                     QLMFGVQRQPFATLAVLSFLGGLLSFASPCTLPILTAYFAFAFQSDRQRIAANTLAFM
                     LGLATTFSLLGATGFILGRVLGQNQGFIMLVGGSAIVIFGVMSLLGRGFTGATSLATE
                     TRSPDMRGSYLFGLTFAIGWSACVGPILGVILTLALQTATVWHGMMLLFIFTLGLGLP
                     LLIVSTFFGRMSRQSAFWRMLRGKGWSWDTHVFVVALVWVLALWRIVAAFARYAIRTF
                     SFLDGVTYTPAMEFGILGLLLVGAILWTVTSKESHRTTVHLHTTQLISGALFILLGLL
                     MLEGQMGLINGMLVRWSAVVDERMLGFQDWLLNAFSQ"
     gene            17985..18659
                     /locus_tag="PLC06_01870"
     CDS             17985..18659
                     /locus_tag="PLC06_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRO89221.1"
                     /translation="MAPLNPQQPQEPEARPVPRGVLIAFGLAALALAGALLFYAYGRF
                     SDVSTREEPLLDPIPAFNATTTSGVAPVQINLDGLPEVGDPAPDFELATLSGDTLRLS
                     DYAGRPVILNFWATWCAPCRLEMPELQRAQTEFDAIENGPIVLAINQGDSAEQVSEFF
                     DEVGLTLPAVLDTEIAVGKTYGAFFLPTTVIVGPDGNVAAVHRGMLSRKELDAYLAEI
                     ASTEGS"
     gene            18656..19414
                     /locus_tag="PLC06_01875"
     CDS             18656..19414
                     /locus_tag="PLC06_01875"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HRO89222.1"
                     /translation="MIIDRIFYTLARRWLLFMNLAVGIYVGLPILAPVLMHVGMTGPA
                     NLIYRAYSPMCHQLASRSFFLFGDQPAYPRAITGSSLTPIEAYMPSIPEFANASPDPA
                     QWSTFLLPARAFRGNEQMGYKMAICQRDIGIYLSILIGGLIYGVLRRRGPVWPLPFWL
                     FLIIGLGPIALDGFSQLFSQMFVGLGIDSLMRLFPLRESSPLLRSLTGAIMGFSVVWL
                     VYPRLDDQFTMTANDIRRRLEAREAAVEEVIPAD"
     gene            19423..20214
                     /locus_tag="PLC06_01880"
     CDS             19423..20214
                     /locus_tag="PLC06_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89223.1"
                     /translation="MRQLQAVDTRRQQSDQLRAELEQARQELVAAEADLAEEQAAVNA
                     FRMHARLMLDDLADAIAALMSEKQSLLTRLELARQAADDEENPLGPDAEEQAAIGDLD
                     DLPIPTITPHDKAAEKRLYRELARRFHPDLAEGAAELAYRTSVMTAVNAAYTAGDVGA
                     LYDLAGELEPGELRELEAIETHEVRRMRERLLQCRRRRRKVTRRLQILREDKTARLWR
                     KARELDERNEDWWSAVRRDLEAARARLEGEVMLLKEQIILSEEAG"
     gene            20620..20922
                     /locus_tag="PLC06_01885"
     CDS             20620..20922
                     /locus_tag="PLC06_01885"
                     /inference="COORDINATES: protein motif:HMM:NF039814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89224.1"
                     /translation="MATTKINMPKGVVERNIALVGQIMQYLMEHPDIFESLPGDFALV
                     ILPEDDPEIRLYNLELLDSYDSEGKPIVFARTRTKTKRGRPAPRPSLFIPIVSVTA"
     gene            20999..21667
                     /locus_tag="PLC06_01890"
     CDS             20999..21667
                     /locus_tag="PLC06_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HRO89225.1"
                     /translation="MKAFRFLSKNWYYAGGVLFVALAFLVGFFGDALDPLRNILLLSF
                     MALLVHQFEEYAIPGGFPAVFNIGLMGEKEFPDRRPLNRKSALIVNVIEGYPFYLLAV
                     IFPGVIWLGLVQILFGMAQFLIHGIVINLRLKTFYNPGLATVVFLHWPIGLYYIWYVY
                     TNGLIQPWHWIVAVACTVLAALSIIVLPIRVFTNTVNDAYAFSAEEMERFHVKEKMAN
                     LVAQ"
     gene            complement(21681..22721)
                     /locus_tag="PLC06_01895"
     CDS             complement(21681..22721)
                     /locus_tag="PLC06_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO89226.1"
                     /translation="MRRNSLPLILSVLALLIVSAACGNNEPPVLIVPAPATSTIAVPT
                     TDSQSSTIAPIVDSPSVAGATIQPTATLPLIPTVAPPLPTDTPDVRPPTATPAPTSEP
                     EAAVDYRVAFVAANDTLNVRRRPGTNAAVIAELPADATGIQVIDEGETLLGDSLWLPV
                     ETAAGDGWVNSRFLTEDIGRDRFCADPEVDELLDVLQKAIANEDGELLRELVHPDRGL
                     RLRINWWNNEILVAGEEVETLFLAPTQYDWGIEDGSGEEIHGSFGEVMMPRLERDLLG
                     ATTWACDEGLFGGTAGSTVLPEEYEAVRFYSAHRAAPADLELDWGTWLIGVERWEGRY
                     YVSYLVHYRWEI"
     gene            complement(22913..24001)
                     /locus_tag="PLC06_01900"
     CDS             complement(22913..24001)
                     /locus_tag="PLC06_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRO89227.1"
                     /translation="MKLGIIGLPSSGKTTIFNALTMGNAPTGQSMGGRFDVLTAIVDV
                     RDPRVDILSRMFNPKKTTYARITYTDVAGLQKSDDDAGGLSPQLLNHLSGLEGFVHVV
                     RAFENASVPHPDGNVDPARDLAALDTEFLINDLGTVERRIEKIQEGLKKGASKDKAAA
                     QAELALFEQMNETLSADRPLRVLDLSEEQLKGLRGYGLLTLKPKIVLINLGDDQTDPA
                     LTYDQPGTILATIRGRLEMELAQLSAAGDEDTLAMFMEEYGVTELSLDRVIRLSYDLM
                     GLQSFFTVGEDEVRAWEVKRGASAVDAAGTIHTDLAKGFIRAEVVHYDDLTGLGGMAG
                     ARNAGKLRQEGKTYSVVDGDILNIKFNL"
     gene            24243..24893
                     /locus_tag="PLC06_01905"
     CDS             24243..24893
                     /locus_tag="PLC06_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO89228.1"
                     /translation="MLVRELMTPKPIVVSPDTTVPDALHLMRDRKVRRLPVVDSHGRL
                     VGIVSDKDLLYASPSPATTLAVWEIPDLLGKLKVEKVMTRDVFTVAEKTTLEEAARIM
                     ADKKVGGLPVMRGPDLVGIITETDLFKSLLELFGGRRSGVRVSVTVPAVKGVLAKVTT
                     AVFEAGGNIVGIGMKGILGSYGDTAEMVLKVQDVDRDALVAVLKPVVNEITDVREV"
BASE COUNT         4467 a         7386 c         7882 g         5062 t
ORIGIN      
        1 gcgaggcggt tgtggccgcc gagggcgccg cgaaggatgt ggagcacaag gcgcgcgagg
       61 atgcgcgggg catcatcgcc acggcgcaat ccgaggcaac cgaggagcgc aaccgcattc
      121 tggccgactt gcgcggccag gtggcggcca tctccatcgc cgcggccaac aagctggtcg
      181 gcgactcgct cgatgagaag cgccagcatg agctgatcaa cgacttcttc agccgcgtgc
      241 cggccggggt gtcggagctg aagggcgagg cggccgaagt gaccagcgcg ctgccgctga
      301 cggatgccga aaagcaatcc gcggccaagg tgctgggcat caaggacgtt cacttcaagg
      361 tcgatccgtc gatcctggga gggctggttg tccgtgttgg cgaccgcgtt gtcgacgaca
      421 gcgtctccaa ccggatgagt gcgctgcagg aatcgctgcg ccactaagca agattggtga
      481 aggagaccgc tcgcggaggc tgctcggggg aaatccctgg ggcatgtctt cgaagagcag
      541 ccggccttca gactctcgtt gagaggggag aagatggcga ccgcgccgaa acggtcgccg
      601 gatatctttg ttgtgaatgc aggccggggt acgactgaat ccccggcctg ttttgttgcc
      661 gggcggcagg cctgtttgac agctgcctcc gcatccggta gcatccggcc tcaggggtag
      721 caatgtcgca agaacaaccc gttccacagc gcgccacgtt gaccgatcag ttgcgggcgc
      781 gcaccaaatt catcatcgac cctgtcgtcg atgtgctggc tcgcttcaag ctggggccgg
      841 atttcctgac tgtcctgggg ttcctgaccc atttcctgtt tgcctggctt atcgccatgg
      901 gggagtttcg ttgggcggcc gtggccatcg cgctcatatc accgctcgac gcgctggacg
      961 gagcgctggc ccgcaaactt ggccggaagc agggcggctt cggcgcgttc ctcgattcca
     1021 gcctcgaccg tttggcggag atcgtcctct ttggcgggtt catctattac tactacagca
     1081 acgtgaaccc gttgatgctg gccgtggcct atctggccgt gaccggctcg ctgatggtca
     1141 gctatgcccg ttcacgagcc gaggcgctgg gcttccaggc caagatgggc ctgctcagcc
     1201 gggtggagcg ctacgtcgtc ctgatagtat ttctggcgct cagaatgccc catatcgccc
     1261 tcggcatatt ggccgccttc acgtatttca ccttttttca gcgcatgtgg gccgtctgga
     1321 agcaatccag agactgagag gacattgacg catgtcccga cgtattgatc cctggtggta
     1381 tgcaattgcg ctcgtcctgc tgttggcggg cgggttatac gcgtaccatt tggccacggg
     1441 cctgacgcca ccgcgagcgg ccgtcaccat cctgggtttt cctgtttact ggtacggcat
     1501 ctggatcgtc acgggtgtgg cgctgggggc gtggctcgtg gctgggttgg cggccgaacg
     1561 cgcgcgccgg atttttgaaa ccaccgtgcc ggtggatgtg cgcgctcgcc ctttggccga
     1621 ggcggggctg cccgatgaag ttgttgccgt gctcgcatcg cgcaatatcg gggcgctggg
     1681 gccgctgctc atggatgtgg gactggaccc acgccggttg gggctgaaga aggcgcagac
     1741 gaccgaaatg gtcacgagcc tggccgccgc gccggacatt gacccggcgt gggtctctga
     1801 tgctccgtgg cgacgctgga accccgaaca cgtgtggaac gcgctgatct ggattctgat
     1861 cctgggcctg attggggcgc ggctttacca cgtgttgacg ccgtcgccca gcatggcggc
     1921 gctcggcatc tattcgccgc tcgactattt ccgcgacccc atccagttga ttaactttcg
     1981 cggcggaggc ctgggtattt tcggcggatt gatcggcggc ttgctgggcc tggtcatcta
     2041 tacccgacgc gctcgcatcc cgtttctcgg ctgggccgat ttggcggtgg tgggtctggc
     2101 gctggggcag gccgttggcc gttggggcaa tttcttcaat caggaactct atggccggcc
     2161 gaccaatttg ccgtgggccg tccatatcga ccccatctac cgcttgcccg attacgtctc
     2221 ctttgagcgc tttcatccgg cgtttctgta tgaatcgctc tggagcctgg ccacattctt
     2281 cgtgctctat cgactaaccc gacgctatag cgagcgactg ctgccggggg agatgatggg
     2341 gctatacctg atcgcctatg ccgtcggccg cagcttgctg gagctggttc gtctcgatag
     2401 ccgggtcatc cctttcttcg gggtggagac cgggctggcc gtcgccacgg ccgtgtccat
     2461 cgttattgcg ctggcgtcgg cggtcatgct gatcggccgt cgatggtcgc gccgccgcag
     2521 aggcgtgggg tgagcgccgg ttaatcgtct ctgacagtcc gcgagacctc tgttaaacta
     2581 ccgctgtgtc gaccaaacgt tataaccctg tccttgtcct tgtcattgcc gcgctcgtcg
     2641 tcttgctggc cgtgctgctc ggcggccgat tgctgaacgg cgacgattcc ctgttgcgca
     2701 acgtcgccat gggcgacagc gccatcacgc cgaacgccga cggcacgacc gatgccaccc
     2761 cgatccgcta tgagttgtcg cgcaatgccg ccgtgtcgat ctatttcgaa gatgcggccg
     2821 gtgatcgatt ctactttcgc caggacaagg cgcgcggcgc gggggagtac gaggtgctgt
     2881 tcagcggcgt tgtcgacggc taccgactgg cgggggacga gatcgagggt gagatactat
     2941 cccgactcct gcatgacggg gaatatacct ggacgatcga ggccactgac tttgacggca
     3001 tcacggagcg gcatcagggc acgatcacca tatccggggc cgatccattg ctgccggaat
     3061 tgcgcaattt cacgctcgac cgccacctct tcacgcccaa tcgcgacggt atcgacgacc
     3121 gggtgctgat gcaatattac ctcccgaagg aagtgatcag cgcgcgggtc tatttgcgac
     3181 gacctgacgg cagcgaactg cccatcgccg agcaggagcg cgatattccc gccaatatgc
     3241 ccggccgtca ttattacgac tatgagggcg gcgtggatca tgatgagacg ccgccgcccg
     3301 acggcacgta tacggttgtg gccgaggcgc aggacgcgga aggccagcgc gtgcgnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagaa tgagttgacc aattacccat
     3481 accgttgggc gctgggcaag ggctcggacc tggaagagat tgacggccat ctgtatctga
     3541 tgcccggcaa gcgcgccgtg atcaccggcg gcattcgcgt cgtggacgtg ttcggcaatc
     3601 gcaacccgca gccgatttgg gctggtctga tccacgaaga cgtgcagatc accgaattta
     3661 ataatcgcgt cgatccgcag tcgatactgg tcgatatccc caatgcggcc gcccgccagg
     3721 attgcgagcc gcgagagata ccggtgcggg tggcggatta gacgacggcc gaccgccggc
     3781 tgcggacggc cgacgacaga ccacgccaat cgctcgcgac ttgccgcttg ctccccttcc
     3841 ccctctctcc ctttctctcc ttccttcttc ccaccttaac ctcccctaaa tgtcgttgga
     3901 gtgccccggt aaacgggtta cactaaacat ggttagcagg ggccggccgc gctcaatcag
     3961 cgaggcgatc tgtgtcgagg attgacgatg atggccggca gatgactggc gacaccgtca
     4021 gtcttttttt gtctatgaag aggatcatca acgtcggagt aatgatggga attgtgcagc
     4081 tggggctgct gctggtcgct tgcaactcgc cggtcgctca gccgccgttg gccggcagtc
     4141 ccgcgattga ggtgacggcg ccggcggtta cgccatcgac ggccgtcccc gttcctgttg
     4201 ccgctcgcac gttggcggca acgcccgctc cccagattca tgaggtcacc ccggcggtta
     4261 cgatgatacc ggtcacggtc gaagtcactc gaccgccggt gggcaccgcc gagcgtcccg
     4321 tgcaattgtt gttcccgcct gtcgccgccg gagccgtgat cgcccagcgc gcggcgccct
     4381 tggtcgcggc gttgcaggcc gccaccggcg cggagtttgt cgtgggggtc gtcgacagtg
     4441 aggccgcatt ggtcgaatta ttgtgtaccg cccccgaaga cgttatcggc tttatctcgg
     4501 ccgcggccta caccatcgcc cacgaccagt gtggcgcaca gcccgggctt gtggccgtgc
     4561 gggaagacgg cctgacgtgg cagatgggga tgctggctat tcgtcccggg ggcgccgcgg
     4621 ggctggccga tctggcgggc aagcgctggg ccgtagccga tacccatagt ctgcccaatt
     4681 atctgtattt cctgtcgcaa atgactgcgg cgggcattgc gccgggcgag atcgtggatg
     4741 cgcctgagga atcctcggcc ttgttgtcgc tctataacgg agaggtagat ttctccacgg
     4801 cgagcttcat cccgcccatc atgccccacg atcggcaatg gatcgccggg gagacggaag
     4861 cggaagagtg gcggctgctc ggtattccgc cgtcgcgtag ccccattggc tatgtggtcg
     4921 tcgccggcga gcctgagttt ggcgggtatc gccttcgcga cgcgcgcgcg cgaatgttcg
     4981 acacgacgcc ggagatattc aacgcgaccc gtatcctggc tctgagcgaa ccgatcccca
     5041 atgataccat cgtgttcggg gccaatttcc cgttggggat tgcccgccag gcgttggcaa
     5101 ccatgaccga gtttgccgca tccgatgcct gcgccacgtc cctgtgttcg gccgacctgt
     5161 tcggctgggc cggcctgcaa ccggccgaag atgctgccta tgacccgatt cgcgttatca
     5221 aagatacgct ggaactggaa gctgccgact tattcgcaga actggactga gatgatcgac
     5281 gtcgccatta tcattgtaag ctggaacgtg cggaactatt tgtccgattg cctgcgttcg
     5341 gtcgcggccg atttgcgcgt ctcgcgcctg cagggtgaaa tctgggtcgt ggataatgcc
     5401 tctacggacg gcacggtcgc cctgctggaa gacttgttcc ctcaggtcca cgtcatcgcc
     5461 aatcagacaa acgccggttt tggcgcggcc aacaatcagg gcatgcgcgc ggcggcggcc
     5521 catcacccgc gctactattt cttcctgaac cccgacacgc tggtgcggcc gggggccatc
     5581 ggccgtctgg tgaaatgcct cgacgaacgc cccaaggggg gcatggccgg cgcgcgcctg
     5641 acctacggtg acgggcgctt ccagcacagc gcgttttctt ttcccgggct gggtcaattg
     5701 gtgttcgacc tctatcagat gccgtcgcga ctctatgaga gccgcctcaa cggccgatat
     5761 ccgcgccgct actatcgcac cgaccgcgcg ccgttcaccg tcgatcaccc tttgggggcg
     5821 tcgatgctgg tgcgagccga tgtggccgaa tcgacccagg gctttgacga gtcgtttcac
     5881 atgtattgtg aggagatcga ctggagttgg cgcgtgcgcg agtcgggctg ggagatttac
     5941 gccgtacccg cggccgaaat cgtccacttc agcggcgaaa gcacccgcca gatcccggcc
     6001 cagtcgatgg tcaacctgtg ggagagccgg gcgcgcctct atcataaaca ccacggccgc
     6061 attcgacagg cattggcccg ctggctggtg cgctcgcgca tggccgcgcg cgctcgccat
     6121 gccggtgatc ccgaactgaa ggcggcatat gagcatatcg tcgacgtctg gtcgatgaac
     6181 ggtaaagtgg gctagccgct gcggcggccg aaaaataaag gcggatgggt caccagacct
     6241 gtccgccttt ttgtttgcga tatcggggtg ttcgggcgtc tactggataa agcgttcgat
     6301 ggtcgcgttg gcccacaagc cttccagcca cacgtcgtaa gccgcttgca tgaggccgta
     6361 gcgatcgtcg gcggtcaact cgcgattggg gtcgcgttcc gtcaccttga tgatgtagta
     6421 agttgtttta ccggcggcat cggtgacggc gatcacgtcg ctgatctcgc ccggccggag
     6481 agcaaaggcg gccgcctcga cgtcgggcac cagcaggctg cccaccctga agaagcccaa
     6541 atcgccgccg ttggccccgg tcacccgatc gaccgagttt tgttccgcca gaaaggcgaa
     6601 gtcatccccg gcgcgggcgc gctccagcac cgattgggcc agcgcgccgt cgtccatctg
     6661 gatgtagctg gcgcgaatgt gttccatcgt cgtcggcacg tcggccgtca cgcgggcgat
     6721 catctcgccg gtgacgatct cggccgccag cgtctcgcga aattcctctt ctgtccagcc
     6781 gttggtctgc aggaaggtct ggaacgcggc ctcatccgcg gcgcgcagtt cggccagacg
     6841 ttggtcgacc atttcgggcg tggcgacgac gccggcggtg gccgccgcct gcatgatgag
     6901 ttggcgctcg atgagcgcgt tgagcacctg ctcgcggacg tcggtcgtct cgctgcccgg
     6961 cgtcgtttgc tgcgtgctat agcgcgccag ctccgcctcg aaatcggaca ggaatatctg
     7021 ctcgccattg accagcgcgg ccagcggctc ggtcggcgtg ggcgagggcg ggacggcggt
     7081 gttggtcgcc gcggccgccg gggggagcgt tgcggcgtct ccgggggcag tggccacggg
     7141 caggagcgtt gtcgatgatc cggcgttgcg acatgccacg gccgacagga gcaaaatgat
     7201 gagcgccaga agcgccggtt ttcgttgagt catagcggtt aattatagcg taatgtcgcc
     7261 tctctatacg attcggccgc gcttgatgac agtctcaacc agattcgtgc cgtagcgata
     7321 gccgacctgc cggtaatcgg cgacattgag gatgaggatg tcggcctgat agccgggggc
     7381 caggctgccg acgtcgtggc cgcggccgat ggcgaaggcg gcgttgagcg tggcggcgac
     7441 gatggcctgg gcctgcgtca gccccatata gcggcaggcg agggcgacga ccatctgcat
     7501 ggattcgcac cacgtcgtgc cggggttcag gtcggtggcc agcgccagcg cgccgccggc
     7561 agccaaaatg gcccgggccg gggtgtactc cttgtgggcc aacccgaacg gagtaccggg
     7621 cagaccaacg gccagcgtgt cgccgcggcc gagggcgatg atgtcggctt cgggtgtcat
     7681 caccagatga tcggccgatg tcgctccgag ttcgacggcc agctttgtac cgcccagacc
     7741 gacaaactca tcggcgtgga ccttgagccg gtagccgagc gcggcggcgg cggtcaggat
     7801 gcgccgcgtc tgctccagat cgaaagcgcc gtcttcgcaa aacacgtcgc aaaatagcga
     7861 gatgccccgc tcgtgcatcc aggccgcccc ggcgggcagc atctcgtcga tgacgagtcg
     7921 gacatagccc tcggtatcgc cgcgatattc ggccggtacg gcgtgggccg ggagaaaggt
     7981 gggcgtcagt tcgaccggct gggtcatggc cagagcgacg atggcgtcga gttgcttgat
     8041 ctcggtcgcc gtgtccagcc cgtagccggt cttggcttcg gcgctggtcg tgccgtggcg
     8101 cagcatgcgc gccaggcgcg gcaggttgtc ggcgacgagt tcgtcaatgc cggcctgccg
     8161 cgtgttgcgc acggtggaca tgatgccgcc gccggcgttc atgatctcca tgtaggtggc
     8221 cccggccagt cgttgctcga attcaccggc gcgatcgccc gcccagggca gatgggtgtg
     8281 cgggtcgacg aagccgggta tgacgcagcg gccgccggcg tcgatggtga tctcggcctg
     8341 gaagcgagcg cgcagttcgg ccgacgggcc gacggcgacg acgcggccgt tgcgggcgac
     8401 caacgcccca tcggggataa ggcccaggtc acccagcgcc gcgccccgtt gcgggccctc
     8461 gtcgtgggtc ggaacaacac agagttgctc cgccgaatga atcagcagat caacaggttc
     8521 actcatggga agcctccttg ccctcgcgta tccagcgagc cagcacgagc acggccagca
     8581 gcatcatgat ggccgagatg gtgctgttgc cggccacccc cgccacctcc caggcgtatg
     8641 gtccaacgag cgagccgatg gtgcgccccg cggccatcgc ccccaggctc atcgacatga
     8701 ccgcggcgcg ttcgctgggc accagttcgg tcaacagggg gatggccccg acgacggcga
     8761 tctcgaaggt gaagaacagg gcgatcatcg ccgccatggc cgtcgccagc gagccggtgc
     8821 tgaaagggat gatcaggaag atgatggcgt taaggatgcc gcacaggata accaccggcc
     8881 gcttgccgaa gcgatcgaca gaccaaccgg ccgtcgcctc gccgcccatt tcggccaggc
     8941 cgatgagcgt ggtggcgaaa ccgaggctgg tcaggctcag attaaaagtg ctttccatcc
     9001 agtcgccata gacgatgaac agggtctcct gggctaccaa aaccagcatc atgtagagcg
     9061 cggcggccca gatgatcggg tgcttgcgca acatctgcca ggtgtagctt atgccgatac
     9121 cgtgacgacg acccttgccg gcggggggca gggcgcgcca cagcatggct gccgccgcga
     9181 ggccacctac tcccagccac aggaagggcg caggccagcc ctggttggcg atcagccagc
     9241 cggtgagggg cgcgccgacg agaaaggccc cgctccaggc gaattcggtg accgacagcg
     9301 ccttaccacg ctggtgatag ggcacgatgt cgccgaggta ggattgcatc gccgggtcgt
     9361 agatgacttt gaggacgccg gccatgatga tggcgatgcc aaacgtccag attaccggga
     9421 cgcccaccac cagcagcgag gcaatgccaa agatcaacag cgaggtcgac atgaccaccc
     9481 ggcggccgta acgctccggc agcggcgcaa acaggggcgc gaacagaccc agcaagttgc
     9541 gcagggagac gataaaatag atggtctcga tgggcacgcc gaggccgcgg gcgaacgccg
     9601 gggcgaaggg gtagacgacg cgcaagagcg tgttctggag aaaacgggcg aaggtcagaa
     9661 cgcccaactg ggcgagtcgc cggccatccc agctccatgt cgcgacggcc gacgcgcttt
     9721 cctgtggttg ggccatatgg cagggagttt aacccatgcg caggggactc acaatgatta
     9781 acggcccggg agacatgtgt tgcgtcagtt gtttctttgc aggagtcatg caatatccgc
     9841 ctttccgcgc ttgatacgtt tatcgttagg gagccttgac agaggtaggg tgactgttat
     9901 actccctttt aatgtcatga cgcacaatgt catggaattg atacgttcgc tgcaatgtgc
     9961 agaacggtta agcgttgaaa cccatacccg ccaccagggt aaccaggaaa ggagatcaga
    10021 tttcgaatga gcgtagccga attatttaac agtatgccgg gtcggcttca ggtcgacaag
    10081 gccgccgggg tcagcatgag catcctcttt gacttgagcg gtgatggcgg cggccagtac
    10141 ctcgtcgacc tcaaggacgg caagttgaac gtggccgagg gcgcgcccgc ggccgccccc
    10201 agcgccaccg tcaagatggc ggccgatgat ttcaaggcga tgtccgctgg cagcctgaac
    10261 ccgatgatgg ctttcatgac cggcaagatt aaggtcgacg gcgacctgaa ctccgtcatg
    10321 aagttccagt cactggttgg gttctaattc tgaagacaga cctgactggt cggcgatgta
    10381 agagcgggag cgatcccgct cttttttgtt ggggacctca acgcgcctgc tcgccgcggc
    10441 ggctgaaata atcgagcagc agggcgtgga cgaaggtgta gccgccaccg accttgcgca
    10501 gcaggttccg ctcggcggcc aggttgagga aagcggtcgc gtcgtcgggg aaatcgcccc
    10561 ggtcatggag aatgctccgc agccgccaat gctgcagcca ggccaggaag ccgaacgaca
    10621 ggccgcagcc cgcgcccaga aacagggcca accacagcag ccatgctgcc gccgggatga
    10681 atgcctgacg gctcgtgacg gccgcggcga cggcgacggc caccgcgccg aggatcaatg
    10741 tcgtggcgat gaccaggccg gcgaccatca ggccattgcg ccgcgactgc tccacgccct
    10801 gaccgggtgt cgtgcgcagc cgcaagtcgc tgcgcagcag ggcgttctcg accattgtaa
    10861 ggacgccgcc ggccacggac aggagtgccg gccagggcgt acttgtccag cgatcgagcc
    10921 aggcgaccag cgcgcccaat cccaacccaa cggccgcgcc ggtgccaaac cccaacgccg
    10981 cccacggcca cgaccagccc agagattcca cggtctcgat gcgcttgaag ggcagccggc
    11041 cgcgcaccag aaagcgcccc gtgatcgccg ggacggccgc ggcgaataac ccgacaagca
    11101 gcagcgcccc cgccgcgccc cagcgattgt acaagagcag caatagcgct cccggcacgg
    11161 ccgctcccgc caatatgccg aaaacggaca ggcgcatctg acgaacgaaa ccgcggccgg
    11221 cgtcatcggg cagccaggac ggctgcatgt cttccaggaa gaaggtaggc ttcatggcgc
    11281 gcgtctgctg tttggccagc cacgacagcc agctgatggt gtcgctgggc gaatagctca
    11341 tgttcttgcc gcggtagcga gccatacgtt ccacgtagac gtcgaagagg tgttggcggc
    11401 tgagcaggcc gtcgcccaga tcggccgcgc cgctctccgg cggccggttg gcggccaggg
    11461 tcatgatcga cagcatgagt ggtgtctggg ccagctcctg gagcgtgtga tcggcggcca
    11521 gcgaggcgcg tagcccatcg agtttattac cccgccgctg cagataggtg tcgatctgct
    11581 ccagggtcag cggttggaga atgatcgcct tgtccatgtg caggcgagtg gccagggcct
    11641 gataatcccg gttgcgggcc gtcaccagca gcggcacgcc gggatggcga gcgcggaact
    11701 cgttaatggc ttcggcgcag gccgggcgat gggccggatc cacctcatcg aggccgtcga
    11761 gcagggggat gaattgtccg gcgtcgatcc attgctcgcc aagcttgcgc gggacctcgt
    11821 agttgccggc caactgctcc accagccatt ggctcagggt tcggccgttg gtataggtcg
    11881 ccaggctgaa gacggccggc atggggcagg tttcatcagc ctcggcctga gccatcaaat
    11941 ggctgaccag ttgcagcagc atggtcgtct tgcccgcgcc gggttcaccc agcacgagca
    12001 ggacgcgatc ggccgcggcg aaaacatctt cgatccgggt tccgacaggc aacaggtagt
    12061 cgtattcggg tgcctgctgc cagctgggca caccgctgtt gcccaacgct gccgggcgat
    12121 aggcgaagcc caggtcgatg atttcgccgc cgtggagcga ctcctcaagg acgccatcca
    12181 gccacaccgt tttcacgcgg cgcagcatga cagttcgatt gtgacgttcc tggtcgttca
    12241 ttttgcctgc ccgcgctttg gttatgattg gctatcgcga gaggggcggg atagtgggga
    12301 attatacctc ctttacagag agggctatac tattcagcga gtgatcccgc ttcatggaaa
    12361 tacgccgatg aatgatgaat tcgcccgcgc gttgtggcgc atctataacc gcccggactc
    12421 gccggcgctg tggcagggtg gcggcaatct gccgtggaac gaccccgatt tctcggagcg
    12481 gatgctgcgc gaacatctgg atgagggaca cggcgcggcc acgcggcagg cggccgagcg
    12541 ggccgcgcaa ctcgattggt ttcaggagca attgggtctg cggccaggta gtcgtgtgct
    12601 cgacctgacc tgtggccccg gcctctacgc ggtggcgctg gccgaacggg gaatgaacgt
    12661 gacgggcgtg gacttcagcc ccgcgtcaat cgcctatgcg cgcgagttgg ccgtcgccgc
    12721 cggcgtgggt gaccgctgtc gattcatcga gactgatgtg cgcgactatg agccggaacg
    12781 ggaaggctac gacgcggcgc tttttctcta tggccaactg gccgtctttc cgcgcgagga
    12841 ggcggcggcg ctgctgggca aggtggcgag cgcgctgcgg cctggtgggt ggctggtcgt
    12901 ggagctgctc gatccggcgc gggtggacaa gagcgatagc tcgtggtggt tcaccgacga
    12961 cacggggctg tggggcgggc ggccgtttct ccatttggga gagcggcgct ggaacgcggc
    13021 cgaacgggcc tcggtggagc gctacactat cctgcatctg gagagtgggg tgatggatga
    13081 gatcatcctc tgcgatcaga cctacgaggc ggaagagatg gcggggatga tgcgcgcggc
    13141 cggattcaat gagatgaaga gcttcgcggc atgggcgggg ctgccgctct atgacgctga
    13201 ggaatgggtg gcctatatcg cgcagcgata gcttacgatg ttgtatcttg tcctgattgc
    13261 tcaaggaaga tgacgaacat catcaattcc gaatagagtt gatcaaacac ggttcgggcc
    13321 tctagagtca gatgacccac tcgatcgccg tccaattgaa aagcatagac gttacggacg
    13381 acatgccgga atcgcagaaa atcatccaga gccgtgcagg tgacttcggt aagcactcgc
    13441 gggcgaatgc caggaagctc gacgcacatt tgtcttaaca aatcgcgatg ccaggcttca
    13501 ctcgccggca tacttctgtc aactgtcgtc acaatttggc gaaaaatccg ttccaggcca
    13561 gtgtagaaat cgtgcaggtt cagtgcaacg gcatcaatat agaggtcgga gtcttcggca
    13621 tgttgttttg ctgcaatatt gcctctctcc gcgcgtgaga tcacctgatt tagttcagcc
    13681 agttcttgag gaattcttgc tgcgagaacc agataatcaa cgttcacagt tctaccccct
    13741 cgctcataat cgactccatc aacgcggggg tggccgtcgc tacatcaacc agattgacat
    13801 ctatccccat ttccatatcc atcattgcgc caattgctcg caatgtatct ttggaatcga
    13861 tcccccaggc agcaatatca acatctgacc accgcgtgaa actaccgtct ctgactaatg
    13921 aaccaaatgc gaccacgcgc gaagcgtgga aatcttgccg tagaagatcg gcggcagcat
    13981 gagcggccgc ccaggccgtt tcccgacgcc gcttctccat ctcttgatcc ttgacgcgca
    14041 gataaacggc attggcacga aaaacagcaa gctcttcctg agaccaaata gattcagcca
    14101 tcaattcaat tataacagga aatcctttgg tcgagtgagt tgtcacatca tagcaatccc
    14161 ctacaattca catccatgat ccgtctcaat ggcgtcgtta aacaatatgg ccttaacccg
    14221 gttctgcgag gcgtcgatct gaacgtcgag cagggggagt ttgtcaccct ggtggggccg
    14281 aacggggcgg gcaagagcac gctgatgggc atcgtcgcca ccttgctgcg gccgacgtcg
    14341 ggcgatgtat tcgttggcgg ctggcggctg ccggagcagg ccgatcgcgt ccggcgccat
    14401 atcggtctcg tttcccacca ttcgctgctc tatcgtgatt tgaccgcggc cgagaatttg
    14461 gctttcttcg ccaaactcta ccggatggaa gacgccgacg agagggtcgc cgacgcgctg
    14521 cacaaggtgg gcctctatgc ccggcagcgc gacccggtgg gcaccttctc gcgcggcatg
    14581 gtgcagcgac tgaccatcgc ccgcgccaca ctccatgagc cggacgtgct gctgcttgat
    14641 gagccgtata ccggcctgga tcaggaagcg agctatctgc tggacgaact gttgcgcggc
    14701 gagacggcgc gcgggcgcac catcctgatg atcacccacg acctgggtca cgggttgggg
    14761 ctggctgatc ggctggcgat cctttatggc ggcattatcg ctcgcgaggt gtcgcgggcc
    14821 gagatcagcc cggcggcggt ctatgacctg tatgcggaag tgacgatggc ctaagcgaca
    14881 atcggctgag aaagcaagag accggatcat gggcgaccca aacctgcatt ctcgcgagtc
    14941 ctgatcagta tgaaagaatt ctgggcggcc gtgtgggccg tagtgtggaa agatctgcgc
    15001 atcgaggggc gaacacgcca gacggtcagc gtcatggtga tgttctcggt ggcgacgatc
    15061 atcatgttca actttgcgtt gggtatggcc gtgaacgtca acctgagcgc gggacgcgat
    15121 gtggccccgg cgctgctgtg ggcgattgtg ctcctggccg ccatcctcgg cctgaatcgc
    15181 tcgctgtctc tcgatcgtga gaaccaggtc ttcgacgcca tgctcatcgc tcccattccc
    15241 cgcacggcgc tctatgcggg caaggtgatc agcatcacgc tcttcacttt gatgctggac
    15301 gccattctcg tcgttctctt caccgttttc ttcaaccagc cgtactatct gccgatgatt
    15361 atgctggtgc tggcgttggg gacgattggc tacatcgctg ccggagtgct catcacctcc
    15421 atgaccattc agagccgcac gcgcgaggtg ttgctgccgg tgctgttgtt gccggtgtcg
    15481 ctgccgctgg tgttgcccgc ggccatggcg accacgacct acatcgcttc tgccatgcgc
    15541 ggggaggcgt cgtggagcct gatccagtcg ccgatgctgc tggtcgttgc ctatgacctg
    15601 atgatgctcg gcgtcggttt tctgacctat cattttgtgg tagagtcctg ataattgcca
    15661 agtggcgagg gacgaaattt gcaaatagaa tggcagcggc gacccttcaa cctgtcacat
    15721 gccgcttccc gtttatgacc tgaacctgat caatcatcca tcattgcggg gtaaccaatt
    15781 atgtctcctg ttagcctttt caatccgagg ctggatcgct ggattcgcat tttcaacgtc
    15841 gcggccgtag tcgccatggt cattgccatc ggggcggcct ttttctacgc tccggttgaa
    15901 atgacgatgg gcaacgtcca gcgcctgttc tacttccacg tcggcgcggc gtgggttggt
    15961 tcggtagtat tcggtgtggc cctgttatgc ggcgtgctct atctgcgcgg cggtcggcgc
    16021 gtttatgacg tcatctcgct ggcttccgtc gaagtcggcc tcgtatttct gacgatgacc
    16081 atcgtcggcg gctccttctg gggcaagccg gcctggaaca cgtggtggat ctggagccca
    16141 cgtctgacgc tcatcaccgt ggcctggctg gtttatgccg cctatttcat gttacggggc
    16201 gcaatcgaag acccgcataa gcgcgcccgc tacgccgctg tctatgccat tgtggccttc
    16261 gtgacgatca tcctgacctt cgtcagcatc cgcatcttcc gcgacattca tccggtggtg
    16321 attggcggga cgaccgaagc ggccgccggc gcggccgagg gcttgcagca gttcaacggc
    16381 cttgattcag ccaagatggg cattaccctg atgatcagcg tcatcgcttt ctccatcctg
    16441 ggcacggcct ggattttggt tcgtatgcgc ctgcaggaca tgatcgacta caccgacaac
    16501 ctgaagaccc gcgtggcggc ctatctgagc agccggcctc gccgcagtcc ctcgccggtc
    16561 agcagcctgt tgctgacttt gcccgcgctt cagccacgta tcgccgatcc caacggcttc
    16621 aaccagtacc ttatcggcgg ctacgtgatc atgtcgggcc tggcgctggc ctacatgctc
    16681 tatctctacg tgcgccagcg caatctgcag aaggacatca acctgctgtc gcaattgtta
    16741 caggaagata aacggccgcg acgctaggag ccgccgaacc gatcaccgtc ccggtcgtgt
    16801 ggtatactct cccgtgtgag aacggcggcg gtgaggtcgt ccgatctcag agagcttttc
    16861 atgtctgatc tgaaggcacc cgcgccgcaa acgcgcaagt tatcgaccac gcagctcgtt
    16921 ctcatcgggc tggccgttct gctggttctc tttctgatcc ttggatcact gattcgcccc
    16981 gacacgagca accagctcat gttcggcgtc cagcggcagc cgttcgccac gttggcggtg
    17041 ctgtcgtttc tcggcgggct gctcagcttc gcctcgccct gcacgctgcc catcctgacc
    17101 gcctatttcg ccttcgcctt ccagagcgac cggcaacgta tcgccgccaa taccctggcc
    17161 ttcatgctgg ggttggcgac gaccttcagt ctgctggggg cgacggggtt catcctcggc
    17221 cgggtgttgg gtcagaacca gggcttcatc atgctcgtcg gcggatcggc catcgttatt
    17281 ttcggggtca tgagcctgct cggccgcggc ttcaccgggg ccaccagcct ggccacggag
    17341 acccgcagcc ccgatatgcg cggctcctat ctcttcgggc tgacgttcgc catcggctgg
    17401 tcggcctgcg tcggccccat cctgggcgtc atcctgaccc tggccctgca aaccgcgacc
    17461 gtctggcacg gcatgatgtt gttgttcatc ttcacgctgg gcctaggtct gccgctgctc
    17521 atcgtctcca ccttctttgg ccgcatgagc cgtcaaagcg ccttctggcg catgctgcgc
    17581 ggcaagggct ggtcgtggga tacccatgtt ttcgtcgtcg ctttggtgtg ggtgctggct
    17641 ctgtggcgca tcgtcgccgc cttcgccaga tacgccatac gcacgttcag cttcctcgat
    17701 ggagtcacct ataccccggc gatggagttc ggcatcctgg gcttattgct ggtcggcgcg
    17761 atcttgtgga cggtgaccag caaggaatcg caccggacga cggtgcattt gcataccaca
    17821 caactgatca gtggcgcgct gttcatcctg ctggggctgc tcatgctgga agggcagatg
    17881 ggcctgatca acggcatgct ggtgcgctgg tcggccgtcg tcgacgagcg catgctcggc
    17941 ttccaggact ggctgctcaa cgctttcagt caataatttt atcgatggct cccctgaatc
    18001 cccaacaacc acaggaaccc gaagcgaggc ctgtcccgcg cggcgtgctc atcgccttcg
    18061 ggttggccgc attggcgctg gctggagcct tgctgtttta tgcctacggc cgttttagcg
    18121 acgtctcgac gagagaagaa ccgttactgg accccatccc cgctttcaac gctacgacta
    18181 cgagcggcgt cgccccggtg cagatcaatc tggacgggct gcccgaagtg ggcgatcccg
    18241 cgcctgattt cgagctggcc acattgagtg gcgacaccct gcgactcagt gactatgccg
    18301 ggcggccggt catcctcaac ttctgggcca cctggtgtgc cccctgccgc ctggagatgc
    18361 cggagttgca gcgagcgcaa actgaattcg acgctattga gaatggccct atcgtcctgg
    18421 ctattaatca gggggattcg gccgagcagg tgagcgagtt cttcgacgaa gttggcctga
    18481 cgctccctgc cgtgctggat accgaaatcg ccgtcggcaa gacctatggc gcgttcttcc
    18541 tgcctacgac ggtcattgtt ggaccggacg gcaacgtcgc cgccgtccat cgcggcatgc
    18601 tctcccgcaa agaactcgac gcctatttgg ccgaaatcgc ttcgacggag ggatcgtgat
    18661 cattgaccgc atcttctata cgctggcccg ccgctggctg ctgttcatga atctggctgt
    18721 cggcatttac gtgggcttgc ccattctggc cccggtgctg atgcatgtcg ggatgaccgg
    18781 cccggccaac ctgatctatc gcgcctatag cccgatgtgc caccagttgg cctcgcgctc
    18841 tttcttcctc ttcggcgacc agccggccta cccgcgagcc atcaccggca gcagtctgac
    18901 gccgatcgag gcttatatgc ccagtatccc cgaattcgcc aacgcctcgc ccgacccggc
    18961 gcagtggagc acgtttctcc tgccggcgcg ggcctttcgc ggcaacgagc agatgggcta
    19021 caagatggcc atctgtcagc gcgacatcgg catctatctg tcgatcctga ttggcgggct
    19081 aatctatggc gtcttgcgcc ggcgcgggcc ggtatggcct ctgccgttct ggttgtttct
    19141 catcatcgga ctgggaccga tcgcgcttga cggctttagt cagctcttca gccagatgtt
    19201 cgtcgggctg ggcatcgaca gcctgatgcg cctgttcccc ttgcgcgaga gttctcccct
    19261 gctgcgcagc ctgaccgggg cgatcatggg cttcagcgtc gtctggctgg tttatccacg
    19321 actggacgac cagttcacga tgacggccaa tgatattcgc cggcggctgg aggcgcggga
    19381 ggcagcagtc gaagaagtga ttcccgccga ctaaccgttt gcatgcgcca attacaggca
    19441 gttgataccc ggcgacaaca atcggatcag ttacgcgctg aactggagca ggcacgacag
    19501 gagcttgtcg ctgccgaagc cgatctggcc gaggagcagg cggccgtcaa cgcctttcgc
    19561 atgcacgccc ggctgatgct cgacgatctg gccgatgcca tcgccgcgct gatgagcgag
    19621 aagcaatcgc tactgacgcg gctggaattg gcgcggcagg cggccgatga cgaggagaac
    19681 ccgcttggcc cggacgcgga ggagcaagcc gcgatcggag atctggacga cctgcccata
    19741 cccacaatca ccccacacga caaggcggcc gaaaaacgcc tctatcgtga gttggcccgg
    19801 cgttttcacc ctgatctggc tgagggcgcg gccgaactgg cctatcgcac gtcggtcatg
    19861 acggctgtta acgctgccta taccgccggc gatgttggcg cgctttacga tctggccggt
    19921 gagctggagc cgggcgagtt gcgcgagctg gaggcaatcg agacccatga ggtgcgtcgg
    19981 atgcgggagc gactcctgca atgccgtcgt cgccgtcgca aggtgactcg ccggctccag
    20041 atattgcgcg aagacaagac ggcgcgtctg tggcgcaagg ctcgcgagct ggacgagcgg
    20101 aacgaggatt ggtggtcggc cgtccggcgc gatctggagg cggcacgggc gcggctggaa
    20161 ggcgaggtga tgcttctaaa ggagcaaatt atcttgtctg aggaagctgg ttagcagatt
    20221 ggacccaagg ggtaatattg gtcaccagcc ctcttaattt gtcggatgcg attagtcgag
    20281 gccattgacg cgcggcaacc ataagcgata gaatgagagg gtgcgcaccg tagaattcag
    20341 gcagctattg agcgaggaaa acgctcttcg agtgcgcttt aaactcgaac acgggcaggt
    20401 tgtcgagttt gttgttcagt tggaatgtct atttgaggaa agttggacag ccgttgttcg
    20461 ctatgacaca gcgcatgggt ttgctcattg cgaccgactc catccttatg agccggccct
    20521 gaaaacaagg attgagacca gggactacaa cgaagcgttg acgatcgctc ttaatgatct
    20581 ttccgctcat tgggagcatt atcgcaggag atacgcagaa tggctacaac aaagataaat
    20641 atgcccaagg gtgttgttga aaggaatatt gctttggtcg gccagattat gcagtatctg
    20701 atggagcatc ccgacatctt tgagtctttg cccggcgact ttgcgttggt gatattgccc
    20761 gaagatgatc cggagatacg tctctataat ctggaactgc tggacagtta tgacagcgag
    20821 ggcaagccca tcgttttcgc ccgtacccgt acaaagacaa aaagggggcg gccggctcct
    20881 cggccgagcc tgtttatccc tattgtttcc gtgactgcgt agggcttgaa aaagcattcg
    20941 caaaacaaag aggaatcatc cgcagattac tcagattaag aaagaataac tttgtgctat
    21001 gaaggccttc agattcctca gcaagaattg gtactatgct ggcggcgttc tgtttgtggc
    21061 attagcgttt cttgtcgggt tttttggtga tgctctcgat ccacttcgca atatccttct
    21121 tctaagcttt atggcgttgc tggttcatca gtttgaggaa tacgcaatcc ccggtgggtt
    21181 ccccgccgtc tttaacatag ggctgatggg agagaaggag ttcccggaca ggcgtccgtt
    21241 gaacaggaag agcgccttga tagtgaatgt gatcgaggga tatccgttct atctcctggc
    21301 ggtcatcttt ccgggggtga tttggttggg gcttgtccag atcctcttcg gcatggctca
    21361 gttcttgata catggcatcg tgatcaactt aaggctgaag actttctata acccgggcct
    21421 ggcgacggtt gtctttcttc actggccgat cggtctttat tacatttggt acgtttatac
    21481 gaatggcctt atacaaccgt ggcactggat cgtggccgtt gcctgcactg tgttggcggc
    21541 actgtcgatt atcgtcttgc ctatccgggt cttcaccaac actgtgaacg acgcctatgc
    21601 attttcagcg gaagaaatgg aacgttttca cgtgaaagag aagatggcaa atcttgtcgc
    21661 tcaataatct gccggctcaa ctatatctcc cagcgatagt gaaccaaata gctgacgtag
    21721 tagcgtcctt cccagcgttc gacgccgatg agccacgtgc cccaatccag ttccaaatcg
    21781 gccggcgcgg cgcggtgggc gctgtagaag cgcacggcct cgtactcttc gggcagaact
    21841 gtggaacctg ctgtcccgcc gaagaggcct tcatcgcaag cccaggtcgt cgcgcccagc
    21901 agatcgcgct ccaggcgcgg catcatgacc tcgccgaatg agccgtgaat ctcttcgccg
    21961 ctgccgtcct cgatgcccca gtcgtattgc gtcggggcga gaaaaagggt ctccacctct
    22021 tcgccggcca cgaggatctc attgttccac caattgatgc gcaggcgaag gccgcggtcg
    22081 ggatgaacca actcgcgcag cagttcgccg tcctcgtttg caatggcctt ctgcaacaca
    22141 tccagcagtt catccacttc gggatcggcg cagaaccgat cgcggccgat gtcttcggtc
    22201 agaaaacgac tgttgaccca gccgtccccg gcggcggtct ccacggggag ccataacgaa
    22261 tcgccgagca gggtctcgcc ctcgtcgatg acctggatgc cggtcgcgtc ggcgggcagt
    22321 tcggcgatga cagccgcgtt ggtgccgggg cggcggcgga cattcagcgt gtcgttggcg
    22381 gcgacgaagg ctacccggta gtcgacggcg gcttccggtt ccgatgtcgg ggccggcgtt
    22441 gcggtcgggg gtcggacatc cggcgtatcg gtcggcaacg gcggagctac ggtgggtatc
    22501 agcggcagcg ttgcggttgg ctgtatcgtg gcccctgcga cagaggggga gtctactata
    22561 ggagcgattg tcgatgattg gctatcagtc gttggcacgg cgattgtgct cgtggctggt
    22621 gcagggacga ttaatacagg cggttcgttg tttccacagg cggccgatac gatcagcagc
    22681 gccagcacgc tcaggatcag tggtaagctg tttcttctca tgacagtgcc ctcgcgtcga
    22741 gccggccgtc ggggaagatg atggcgcggg catatcttcg gccggcgatc gaatcgctac
    22801 gtctatcagg tagacgacga accggcgaaa gaggttccat ttgtaccggc gcaagcatca
    22861 gtcttgcgtc gtacaaatga ggcggggcac gcaacttgcg caccccgtga tcctaaagat
    22921 tgaacttgat gttcaatatg tcaccatcga ccacagagta ggtcttgcct tcctggcgta
    22981 gcttgccggc gttgcgcgca ccggccatgc cacccaatcc ggtcaggtcg tcataatgga
    23041 cgacctcggc gcggatgaag cccttggcca ggtcggtgtg gatggtgccg gcggcgtcga
    23101 cggccgaagc gccgcgcttc acttcccagg ctcgcacctc gtcctcgccg acggtgaaga
    23161 aggattgtag tcccatcagg tcatagctga ggcggatgac gcggtcgagg ctcagctccg
    23221 tcacgccata ttcttccatg aacattgcca acgtatcttc gtcgccggcg gcgctcagtt
    23281 gggccaattc catctccaga cggccgcgaa tggtggccag gattgtcccc ggctggtcgt
    23341 aggtcagagc gggatcggtc tggtcatcgc ccaggttgat gagtacgatc tttggcttca
    23401 gcgtcagcaa gccgtagccg cgtaaccctt tcagttgctc ctcggacaga tcgagaacgc
    23461 gcagagggcg gtcggccgag agcgtttcgt tcatctgctc gaaaagcgcc agttcggcct
    23521 gggcggcggc cttgtccttc gacgcacctt tcttgaggcc ttcctggatc ttctcgatgc
    23581 ggcgctcgac ggtgcccaga tcgttgatca ggaactccgt gtcgagggcg gccagatcgc
    23641 gggccgggtc gacgttgccg tcagggtggg ggacgctggc gttctcgaag gcgcgcacga
    23701 cgtggacgaa cccttccagc ccggacaggt ggttgagcag ttgcgggctg aggccgccgg
    23761 cgtcatcgtc gcttttttgc agcccggcca cgtcggtgta ggtgatgcgg gcgtaggtgg
    23821 tctttttcgg gttgaacatc cggctgagta tgtcaactcg tgggtcgcgg acgtcgacga
    23881 tggcggtcag cacgtcgaag cggccgccca tgctctggcc ggtgggggcg ttgcccattg
    23941 tcagggcgtt gaagatggta gtcttgcctg atgagggcaa gccgataatg cctaatttca
    24001 taggggccga gtatagcagg aaataatgaa atggaacggg ggcgttattg tctcaataac
    24061 gatatgatcg aaaggagatt gactgtttca aagcaagcct atgatataat tgatcacagg
    24121 ccaggagggt tgccttggct ggtcgtgaga cttacgggta acctggctta accgaaacag
    24181 aaaagtgaga gggactctgc ctcgcgcttt ttaaaatatt ctcccaatat caggaggttc
    24241 tcatgctcgt ccgcgaactt atgaccccca aacccattgt tgtcagtccc gacacgacag
    24301 tgcccgacgc gctgcatctg atgcgcgatc gcaaggtgcg ccgtctgccg gtggtcgatt
    24361 cccacggccg cctcgtcggc atcgtctccg acaaggattt gctctatgcc tcgccttccc
    24421 cggccacgac gctggccgtg tgggagatcc ccgatctatt gggcaagctc aaggtggaga
    24481 aagtgatgac tcgcgacgtc ttcactgtgg ccgagaagac gacgttggaa gaagccgcac
    24541 ggatcatggc cgacaagaag gtcggcggtc tgcccgtcat gcgggggccg gatctggtcg
    24601 gcatcatcac cgagacggat ctgttcaaga gcttgctgga gctttttggc ggccgtcggt
    24661 cgggcgtgcg cgtctcggtc actgttccgg cggtcaaggg tgtactggcg aaggtcacca
    24721 cggccgtctt cgaagccggg ggcaatatcg tcggcatcgg catgaaaggc atcctcggct
    24781 cctatgggga cacggccgag atggtgctca aggtgcagga tgtggatcgc gacgcccttg
    24841 tggccgtgct caagccggtg gtcaacgaga tcacggacgt gcgcgaagtg taattgc
//
LOCUS       DAOMQG010000014        24614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_876_length_24614_cov_10.960625, whole genome shotgun sequence.
ACCESSION   DAOMQG010000014 DAOMQG010000000
VERSION     DAOMQG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 24614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24614
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_24614_cov_10.960625"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1038
                     /gene="aroF"
                     /locus_tag="PLC06_01910"
     CDS             19..1038
                     /gene="aroF"
                     /locus_tag="PLC06_01910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRO89229.1"
                     /translation="MIVIMQAEATMREKSAIIARAEDFGLKVHLSDGKERTIIGIIGD
                     DRVIDREQIERMPGVERVVPILKPFKLASREFKAEDTVFPLGNHTIGGDEVLVMAGPC
                     SVESRTQIIETAIACKEAGAHILRGGAFKPRTSPYAFQGMGEEGLRYLAEAREITGLP
                     IVTEVMEPALVPLVCEYADVLQVGARNMQNYALLHAVGESQYPVLLKRGMSSLIEEWL
                     MCAEYILSHGNTRVMLCERGIRTFESYTRNTFDINAIPVAKHLSHLPVVADPSHATGK
                     WEYVAGAAKAAVAAGADGLIIEVHPRPEEAMSDGGQSLKPEKFAKLVVEMKAIAQAVG
                     RDIKP"
     gene            1199..2305
                     /locus_tag="PLC06_01915"
     CDS             1199..2305
                     /locus_tag="PLC06_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89230.1"
                     /translation="MARRAVFPFTAIVNQERMKRALILNAVSPRIGGVLIRGERGTAK
                     STAARALAALLPDITVLAGSRYNCDPLRPDEWSDDSHARYDHRPLETEIRQTPFIDLP
                     VSATEDRVVGTLDIEKAIKSGEKHFEPGVLASANRGVLYVDEVNLLDDHVVDLLLDAA
                     AMGVNIVEREGISFSHPAKFILVGTMNPEEGDLRPQLLDRFAFSVQIEGLDDPEDRVA
                     IIERRIAFEVDPEGFRHQWAKNERDLSERIARARDRVNEVRYSRHDLMSIAGLVSSLN
                     VDGHRADLVILRGAQAHAALQGRDRITDEDIILAAELALPHRIRGRVFQDSVLSAAEL
                     SERLDEVQGEMGASDKGQQEQEAEDSAATGKKKP"
     gene            2563..3255
                     /locus_tag="PLC06_01920"
     CDS             2563..3255
                     /locus_tag="PLC06_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO89231.1"
                     /translation="MRDLAFDATLRAAAPHQIRRDRSKLALALEKEDLREKVRVRRSA
                     NLILFVVDASWSMAVAERMEATKGAIMSLLTDAYQRRDRVGLIVFHKNDANLVLPPTN
                     SVQLAKKALINIAVGGKTPLSAGLYLAYQVFRQEAYNHPDVLPLMILLTDGAGNVSMT
                     DMPPQDEAHKIAELISETDVRSVVINMEHAAFDQGFARQLAERLGAPCHTLEDLKADT
                     LYRTVLDQLVTN"
     gene            complement(3260..4393)
                     /gene="dnaN"
                     /locus_tag="PLC06_01925"
     CDS             complement(3260..4393)
                     /gene="dnaN"
                     /locus_tag="PLC06_01925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRO89232.1"
                     /translation="MKISCLQENLAKGLSIVGRAVSPRSTLPVLGNVLLAVDNGRLKL
                     SATNLEVVITCWIGATVSEAGATSVPARTFSELINTFPQEKVELSLNKTTETLHVSCG
                     RTQANVKGINANEFPVIPAPDQENRIRMEPAVLKRVISQVAFAAATDDSRPSLTGVLT
                     TFEGNRVSMAATDGFRLSVRSAHIAGYVEKPFNVIIPARALNEVARIVTDDLEAVYIS
                     MPPGRNQIIFDMDNVLLVSQLIDGKFPDYQPIIPRSHNTRTVLNTADFSKACRTADIF
                     ARESSNTARVSVEPGDGLSPAYAIIAANSKETGDNVAQVDANVEGMPIEIAFNVKFMA
                     DALGVIDTPQVALETTNPNEPGVLKPVGDNDFVHIIMPMNFNR"
     gene            complement(4530..5057)
                     /locus_tag="PLC06_01930"
     CDS             complement(4530..5057)
                     /locus_tag="PLC06_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRO89233.1"
                     /translation="MRPLNGTDLKRFLRDYRRAHKPAGDLIALLQSVEYPANVGSIFR
                     LADGAGVSELILTGITPTPPNATIDKVGRYKSLRLPWRYEADPLPAIEGLRGKGYGIV
                     GIELTEDAVPYHEFNYPDKTCLIVGHEDHGVTKATLAACDAAVFIPMYGKGLSLNVHV
                     ALAIVLYHIRHSTAD"
     gene            complement(5142..6815)
                     /gene="ilvD"
                     /locus_tag="PLC06_01935"
     CDS             complement(5142..6815)
                     /gene="ilvD"
                     /locus_tag="PLC06_01935"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRO89234.1"
                     /translation="MLNKYSSRITQPASQGASQAMLYGTGLTEEDMNKPQVGIAAMWY
                     EGNTCNMHLNQLSAWVKSGMADAGLVGMQFNTIGVSDGISMGNDGMSYSLQSREIIAD
                     SIETAMGAHWYDALVAIPGCDKNMPGCLIAMGRLNRPALMVYGGTIRAGCTPQHPQLD
                     IVSAFQSYGQAIAGSITEEDRRAIVRHSCPGPGACGGMYTANTMASAIEALGMSLPYS
                     SSTPAESAEKKEECVRAAAAVRLLIERDIKPRDIMTRAAFENAMVIVMALGGSTNAVL
                     HLIAMARSVDVDLSIDDFQTVSDRVPFLADLKPSGAFVMEDMQGIGGTPGLMKYLWEN
                     GFINGDCLTVTGKTIAENLAEVPGLSEGQRIIRPLDNPIKATGHIQILRGNLAPEGSV
                     AKITGKEGNRFSGPARVYDAEEEMLAGLEKGQIKKGDVVIIRYEGPKGGPGMPEMLTP
                     TSAIMGAGLGKDVALLTDGRFSGGSHGFIVGHITPEAQEGGPVALVRDGDIVTIDAEK
                     RIIDMAVTEEELAARRAAWSAPPLKVTRGTLYKYVRLVKSASEGCVTDC"
     gene            complement(6880..8079)
                     /gene="lhgO"
                     /locus_tag="PLC06_01940"
     CDS             complement(6880..8079)
                     /gene="lhgO"
                     /locus_tag="PLC06_01940"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HRO89235.1"
                     /translation="MTSTDIIVIGGGIVGLATARSISHRYPNKHITVLEKEQTLAFHQ
                     TGRNSGVLHSGIYYKPNSLKATTCRTGKRMMEEFCREEGIPYSLVGKVIVATDEKELP
                     ALNNLFERGRANGVDCEIIDRERLLEIEPHVLNGIKAIHVPEAGIVDYVQVCERFAAQ
                     LREAGHSIVTGAKVTEINEAGDGLTVITTAGEFKASYLVNCAGLQSDHVTKMSAGEAP
                     AKIIPFRGEYFELKPEAWHLCNALIYPVPDPNFPFLGVHFTRMISGGVECGPNAVLAF
                     AREGYHLSDINAGELWEVLSYPGFRKLAGRYWKMGLGEIWRSASKAAFVKALQRLIPD
                     IQGEHLEEAPAGIRAQALGPDGALLDDFAFYESSRIVNVINAPSPAATSSLSIAEAVV
                     DKLEGHF"
     gene            complement(8134..9216)
                     /gene="leuB"
                     /locus_tag="PLC06_01945"
     CDS             complement(8134..9216)
                     /gene="leuB"
                     /locus_tag="PLC06_01945"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRO89236.1"
                     /translation="MNATISLLPGDGIGPEIVAEARRVLEAVAAAGNHTFSFVEGLIG
                     GCAIDATGSALPSNTLDACLAADAVLLGAVGGPKWSDPKAPVRPEQGLLGLRKAMGVF
                     ANLRPVTVADALIESSPLRPERVRGVDIMIVRELTGGLYFGKPQGPEMVDGERAAVDT
                     LRYKESEIRRVLTLAFELAKGRRKKVTSVDKANVLASSRLWREIANEVAAGYPDITCE
                     HILVDACAMYLINRPADFDVIVTENMFGDILSDEASMITGSLGMLPSASLGNPGGPGL
                     YEPIHGSAPDIAGKGIANPLATILSAAMLLRSSLGLTDEAAAIESAVAQVLADGARTA
                     DIARSGEPTIGTKEMGQRVIAALRIA"
     gene            complement(9282..9776)
                     /locus_tag="PLC06_01950"
     CDS             complement(9282..9776)
                     /locus_tag="PLC06_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRO89237.1"
                     /translation="MSESNLHPSAQRVAAAAEALGLTIDIVTFKESTRTAEDAAAAIG
                     CTVGQIVKSLIFTVRGEPVVALVSGSNQLDTRKLAALFGVGRKQVERANADTVRAATG
                     FAIGGVPPFGYTGSPAMYIDEDLTHYEVIWAAAGTPNTVFSIKPDKLVHATGGTVANL
                     RADA"
     gene            complement(9785..11335)
                     /gene="cimA"
                     /locus_tag="PLC06_01955"
     CDS             complement(9785..11335)
                     /gene="cimA"
                     /locus_tag="PLC06_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRO89238.1"
                     /translation="MTIYLYDTTLRDGTQREGISLSLDDKLKIAARLDAFGMHYIEGG
                     WPGSNPKDVEFFRRAPSLGLSHARLAAFGSTRRKNTRPADDANIQALVGANTPVVTLV
                     GKSWDLHVIEILEATPAENQAMIEESVAYCKSLGKEVIYDAEHFFDGYKADPEHALAT
                     LEAAARGNADCIVLCDTNGGSLPWEIEHAVRQAIERLGDVPVGIHTHDDGGCGVANSL
                     AAVRAGAIQVQGTINGYGERVANANLCSIIPDLQLKMGLECVSAEQLVQLTDLSRYVA
                     ELANLTHDDHLPYVGGSAFAHKGGIHVAAMLKHAASYQHIDPVQVGNQQRSVVSELSG
                     RGNLVDKIRQYELNPDNIDVPKVLDQIKQLEAQGFTFEGAEASVELMLRRTHPAYVPP
                     FEMTDYWVMVQRRRARGTIVEATVKVRVGPKIMHTVAEGNGPVNALDAALRQALVDVY
                     PQISGVRLEDYKVRILDGENNTAATTRVLIETRQGTRRWSTVGASPNIIDASWRALAD
                     SMEYALIH"
     gene            complement(11332..11952)
                     /gene="leuD"
                     /locus_tag="PLC06_01960"
     CDS             complement(11332..11952)
                     /gene="leuD"
                     /locus_tag="PLC06_01960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRO89239.1"
                     /translation="MEPFNKLTAQVVAIPADHIDTDQIIPARFLKTISKTGLGKNLFA
                     DWRYDSDGAPNRDFPLNQPQHEGAQILLAGDNFGCGSSREHAPWALTDFGFRAIISTS
                     FADIFRNNALKNGLLPLVVDDDTHRQLMSLAAEEPDLELSIDLDTQSLILPDGRRVQF
                     PIDAFNKTCLLEGLDQLGYLLKHKPEIEAYETGHGTRVNTLAHPLS"
     gene            complement(12030..13442)
                     /gene="leuC"
                     /locus_tag="PLC06_01965"
     CDS             complement(12030..13442)
                     /gene="leuC"
                     /locus_tag="PLC06_01965"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRO89240.1"
                     /translation="MTDLKPRTIIDKIWDNHIVAQQPNSPVVLYIDLHLIHEVTSPQA
                     FSGLRERGLVVRRPQQTVATIDHSVPTTPLDVPIFDQMAAAQIAQMEKNARDFGIQLL
                     GMHSPNRGIVHVIGPELGLTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQ
                     CLLQVKPKTYAVNVTGQLKPNVTAKDIILALLAKIGVGGGTGHIFEYRGSGIRGLSME
                     ERMTICNMSIEGGARAGLVAPDDTTFDYLHGRPFAPQGADWDAAVANWRQLPTDEGAL
                     YDKSVDIDAGALEPMITYGTNPGMGIPISAPVPNPEDIRDLTQRLTLQRALDYMSLEP
                     GRPLLGHPVDVVFIGSCTNSRISDLREAARLMDGRSVKDGVRVMVVPGSQDVKRQAEA
                     EGLDRIFKAAGAEWREAGCSMCIAMNGDQLSPGQYAVSTSNRNFEGRQGKGGRTFLAS
                     PLTAAATAVTGVITDVREFA"
     gene            complement(13501..14256)
                     /locus_tag="PLC06_01970"
     CDS             complement(13501..14256)
                     /locus_tag="PLC06_01970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89241.1"
                     /translation="MTDPRQDTVWQAIGLADTYLDGVRGAIPFASAQIDILIRLALAA
                     QPAATALLDLGCGDGILGHALLDAYAGKVDTAVFADFSPRMLEVARDRLSGYDGLFLE
                     IDYAQPDWAEVVAKSLPNGSDGFDVIVSGFSIHHQPDERKREIYAEIYALLSPGGLFL
                     NLEHVASATRWVEEQFEEYFIDALYRHHAPNGKTRDEVAAEYYYRPDKSANILAPVEL
                     QCDWLREIGYEHVDCYFKAFELALFGGMKPASS"
     gene            complement(14256..15911)
                     /locus_tag="PLC06_01975"
     CDS             complement(14256..15911)
                     /locus_tag="PLC06_01975"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRO89242.1"
                     /translation="MTQQMPTTDSILIFDTTLRDGEQSPGATLNVEEKLEIARQLSRL
                     GVDICEAGFPIASPGDFEAVRRIAEEVGSLVEGRKDGRPMVIAGLARANKEDISRAYD
                     AVKAAPRHRIHTFLATSDIHLKHKLRIDREECIDQVIKAVSFARSLCDDVEFSPEDAG
                     RSDPEFLVQVLGEAIKAGATTLNIPDTVGYTTPDEFGNLIHYLIRNTPGSDKVVWSVH
                     CHNDLGLATANTLAGIQAGARQAEVTLNGIGERAGNTALEEVVMALTTRPQNFNLTTN
                     IDTTQIARTSHMVSTYTGMVIQPNKAIVGANAFAHEAGIHQDGMLKNHQTYEIMRPEV
                     VGLNTSKLVLGKHSGRHAFRAHLHEMGYDNMDDDEVAAAFKRFKRLADKKKVVTDADI
                     EAIIADEIYQPPVIWQLEHIQVSCGDHSIPTASVVMIGPDGEQYQDAALGSGPVDAVY
                     QAINRIIGVTNRLTEFSINAVTEGLDAVAEATIRIQPDSGTDFTVNPQTDKPVYRTFS
                     GHGASTDIVVASARAYLSALNKMLAERDGYVDTLQPATNGAAK"
     gene            complement(15987..16988)
                     /gene="ilvC"
                     /locus_tag="PLC06_01980"
     CDS             complement(15987..16988)
                     /gene="ilvC"
                     /locus_tag="PLC06_01980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRO89243.1"
                     /translation="MATVYYDQDADLSVIQNKKIAIIGYGSQGHAHALNLRDSGMDVR
                     VGLYEGSPSWAKAEAQGLVVKSSAKAAAEADIIMILAPDTSQARIYREAIAPHLSPGK
                     TLMFGHGFNIRYAQIVPPAGVDVSLVAPKSPGHRVRELYVDGVGTPGLMAVHQDATGN
                     AAATALAYAKGIGCTRAGVIETTFTEETETDLFGEQVILCGGVSHLIESAFNTLVEAG
                     YQPEIAYFECLHELKLIVDLIYQGGLNYMRYSVSDTAEHGDYSAGPRIVTDQTRKAMK
                     EILADIQSGAYAEGWIDENAQGRPWFNQRREEARNGQVEQVGRELRKMMPWLNAKEV"
     gene            complement(17066..17653)
                     /gene="ilvN"
                     /locus_tag="PLC06_01985"
     CDS             complement(17066..17653)
                     /gene="ilvN"
                     /locus_tag="PLC06_01985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRO89244.1"
                     /translation="MSDLQIRRHTIIAWLEDKPSVLNRVTGLFSRRNFNIESLNVGHT
                     ETAGISRMTFVARGTERDLQQITTQLDKLINVVHIEDVTNEPAVVREMAFVKVRTTSQ
                     TRGEVMQLVEIYRASIVDVDMSSVIIQIVAPEDRVNSMIGLLENFGIEEMVRTGRVAM
                     VRGSSSHGHSHNASEWTTAPSATVPVVAERNGHRD"
     gene            complement(17667..19373)
                     /gene="ilvB"
                     /locus_tag="PLC06_01990"
     CDS             complement(17667..19373)
                     /gene="ilvB"
                     /locus_tag="PLC06_01990"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRO89245.1"
                     /translation="MSELKTGGQIIWESLIHEGVDVVFGLPGGAILPTYDDLYKYDYP
                     IHHVLVTHEQGAAHMADGYARATGKTGVCIATSGPGATNLITGLATAYMDSTPVVAIT
                     GQVSTALLGKDGFQEADIRAISMPVTKHNYLITDIKDLPAALKEAFYIARTGRPGPVL
                     VDICKDVQQATMEFTYPTEVYLPGYRPNILAPDPGVIDRVARLINEAQRPVIVAGQGV
                     SMARAEAELRQLAEKANIPVATSLLGIGAIPATHPLSISWGGMHGEAYCNYALQECDV
                     MFAIGARLDDRLTGAFSTFAPKARIIHVDLDPAEMGKNLTIDTPVVADALMTLRALLP
                     KVEPNEHPAWLGQINEWRSESVERDILTQETDELVAPYVMRQLWYATNQGDATIITDV
                     GQHQMWEAQYFNHTKPRSLLTSGGLGTMGYSLPAAIGAQIGNPQEEMWVVAGDGGFQM
                     TMIELSTIMQERLPIKIAIINNGYLGMVRQWQDVFYNKRYVGTPLYNPDFVKIAEAYG
                     IPARAVTSKEDVYQAIRDAQETDGPYLLDFQVEEFTNVYPMVAPGKSNADMIRRGRPV
                     EN"
     gene            complement(19882..21549)
                     /locus_tag="PLC06_01995"
     CDS             complement(19882..21549)
                     /locus_tag="PLC06_01995"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO89246.1"
                     /translation="MRTDSPPAGINIGDVLRLALPIDTTLIGGSAEARRFVNWVVILA
                     SWEDLESQIDPHDLVIVPPNLSEVPDSGKLTTALEVLANLPIAGLVCFSNELAHRAEA
                     HNLTVLIAPENASVREIHHNVSVLLLDRQTATSERAIQLYRQLSAMSREGQGLAAMTE
                     VMSKLTGNIVVVQDKRLEIQAISWPSNTIVNREALVEALQQRDALPPVLRNRKAAAKS
                     RQSIWQQLLPLGDSSVHLGRLLSPIISGDRARGYLSVIGPVAELDMFDSLTVEHGAAA
                     CALEMAKAKAVNEAKKSLRGDFLEGLLAGSLPRKEIERLEGRLDHNTSEPHAVMVAGW
                     IGANKLSLRRLETSTHWVLSNHPRPALVHIYGNQYLVIFQALKNPEDMDSAHQLGRRI
                     REQIEMEYPEATLIGGISGPAKTLSEWPAAYDQALQAMQLGERLKLTNQFVEFDSLGI
                     YRLLYTLEEQPEVREFADEIMQPLIEYDNQNRGSLVKTVEAYFDHHGNISQTAESLFV
                     HRNTLLYRMERIEELTGLNLDHANMRLALHLSLKLWQLRPDSDQPSA"
     gene            complement(21651..23834)
                     /locus_tag="PLC06_02000"
     CDS             complement(21651..23834)
                     /locus_tag="PLC06_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO89247.1"
                     /translation="MDKTIGTEVEERLIGPSAQKRADNGTGGTATGLPKGRPAGGASP
                     VSMSSQSTARILVVDDEIEIADSLAEFLIRKEGFQVDIVHDGQEAIAFLEKSIGGLAE
                     IDLVLLDMRMPGISGLEVLDWIRGHPDLRYTRVVLLTAAASNDEKVQALSAGADDYIT
                     KPYYMQELLARVKTILRTQQLEKQLHQQSRQLAELNRISQAVAATLETGQVYATAIRG
                     VDAILQVEMAAVLIGEGGRLHCQHVRHYKGAIPSHIFPSTEKGAGVLGRAWSDQVTLC
                     LNNVAEDEQYDPTRDSPIGIPVRSMLATPLAVRGRSVGVLAAYNKRSGEFTDVDIDLF
                     SSLASSVSEAIENAWLFQRVRLRHQELFEGRNTLQALIDGIPNPIYTINDEWKLVVIN
                     KAKADELGSTPESFTGRTCFRVFYNRQKPCDHCKVAFTLNDKTEQHWPVRWQGEDHLP
                     REWEVSAYPIPGKQASSARAVVVWQDKTEERRLENSLMQAGKLAAIGQLAAGVAHEIN
                     NPLTAINANAQMLKMVMPVEDENFESVDLIVRAGERAAQVVRGLLDFARQEQYSFTTG
                     DLGKSIQEALALVNYQLQSSKITVNRDIDASLPQMVASWEHLKSVWLNLLLNARDALL
                     HTNDNRRLDVIARPLKDGQSVQVLLRDNGKGMNSAESLHIFEPFYTTKGPGQGTGLGL
                     ATCHRIVEQHGGEITVSSALGEGTTFFITLPVHRPDAGASGFYIK"
     gene            complement(23827..24540)
                     /locus_tag="PLC06_02005"
     CDS             complement(23827..24540)
                     /locus_tag="PLC06_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89248.1"
                     /translation="MALILVIDDDDLVSRTLQRALKMYDYQVMTANSGIEGLQLARRH
                     RPDLFILDIMMPGVDGYQVCRQIRGDPLLQDVPVLFLTAKAKDEDKIEGFRAGGDDYL
                     VKPFNMQELELRVKAILRRIGPEKIEDNTINEVVVGNVVLDCRTFKVTTPYQTILLTN
                     VQFDLLYHLMSNADQVFNSQQLLQDVWDYPRDTGSPELVRAHIKNLREKIEPRPADPM
                     YIKTIQGHGYSFSSEETRG"
BASE COUNT         4920 a         6949 c         7218 g         5527 t
ORIGIN      
        1 ataaggattt atttaatgat gattgtaatt atgcaggcgg aagcaaccat gcgggagaaa
       61 tcggccatca ttgcgcgggc agaagatttt ggcctgaagg ttcatttatc tgatggcaaa
      121 gaacgcacca tcatcgggat tatcggcgac gaccgcgtga tcgatcgcga gcagattgag
      181 cggatgcccg gcgtggagcg tgtagtgccg attctgaaac ccttcaagct ggccagccgg
      241 gaattcaagg ccgaagacac tgtgtttccg ctgggcaatc atacgattgg tggcgatgag
      301 gtgctggtca tggccgggcc ttgctcggtc gagagtcgca cccaaatcat cgagaccgcc
      361 atcgcctgta aagaggccgg ggcccatatc ctgcgcggag gcgcttttaa gccgcgtaca
      421 tctccctatg cgttccaggg aatgggggaa gaggggctaa gatatctggc cgaagcgcgc
      481 gaaatcacgg gtttgcccat cgtcactgag gtgatggagc cggcactggt tccgctggtt
      541 tgtgaatatg ccgacgtgct gcaggttggc gcgcgcaaca tgcagaatta tgcgctgctc
      601 catgcagtcg gcgaatcgca atatccggtt ttgctgaagc gtggtatgag cagccttatt
      661 gaggagtggc tgatgtgcgc cgagtacatc ctcagccatg gcaacacacg cgtgatgctt
      721 tgcgagcgcg gcattcgcac ctttgagtct tatacccgca acacgttcga tataaacgcc
      781 atcccggttg ccaagcatct gtcccacctc cctgttgtgg ccgatcccag ccatgccacc
      841 ggcaagtggg agtatgttgc cggcgcggcc aaggcggccg tcgcggcggg agccgatggc
      901 ctgatcatcg aggtgcatcc gcgccccgaa gaagctatgt ctgatggtgg ccagtcgcta
      961 aagccggaga agttcgccaa gctggtggtg gagatgaagg cgattgcgca ggcggtcggg
     1021 cgcgacatca agccataaca acctgatccc cccgttaccc ctgagatatt cccgcgcccg
     1081 ttttaggtca ccggccattc ccaagttgga gcgccggggc tgttgggata taatctcatg
     1141 agtggaaccg gggtcttctc cccatcgaat ccccatatga ttggagtcaa ttagtttcat
     1201 ggcacgtcgc gctgtttttc catttacagc aattgttaac caggagcgca tgaagcgcgc
     1261 gctgatcctg aatgcagtga gcccccgtat cggcggggta ttaattcgtg gcgaacgagg
     1321 cacggccaag tcgacggccg cccgcgcgct ggccgccttg ctgcccgata tcaccgtgct
     1381 ggccggcagc cggtataact gcgacccgct gcgacccgat gagtggagcg atgacagcca
     1441 cgcgcgttat gaccaccgcc cgctagagac agagatccgc cagacgccat ttatcgatct
     1501 gcccgtcagc gcgacggagg atcgcgtcgt cggcacgctc gacattgaga aggcgatcaa
     1561 gagtggtgag aagcatttcg aaccaggcgt actcgcttcg gccaatcgcg gcgtgctgta
     1621 tgtcgatgag gtgaatctgc tcgatgatca tgttgtcgac ctcttgctcg acgcggcagc
     1681 catgggcgtc aatatcgtcg aacgagaggg gatcagcttc tctcacccgg ccaagttcat
     1741 tcttgtcggc accatgaacc ccgaggaggg cgatttacgt cctcagctgc tcgaccgctt
     1801 tgccttttcc gtccagatag aggggttgga cgatcccgaa gatcgcgtcg ccatcattga
     1861 acgacggatc gcgtttgagg tggatcctga aggatttcgc catcagtggg ccaagaatga
     1921 acgcgacctg tccgagcgta tcgccagggc gcgcgaccgg gtaaatgagg ttcgctattc
     1981 gcggcacgat ctcatgtcga tcgcggggct ggtttcctct ctcaatgtgg acggtcatcg
     2041 cgccgatctg gttattctgc gtggcgctca ggctcatgcc gcgctccagg gtcgcgatcg
     2101 gatcaccgat gaggacatca ttctcgctgc cgaattggcc ttgccccatc gtatccgcgg
     2161 ccgtgttttt caggacagcg tgctctccgc ggccgaatta tcggaacgcc tcgatgaagt
     2221 tcagggcgag atgggcgcga gcgacaaggg tcagcaggag caggaggccg aagactcggc
     2281 cgccacggga aaaaaaaagc cttaggcgag gatgatgaag tcagtgatgg ggccgaagtc
     2341 tcccaacagg aggccgggcc ccagtcagtc cccaaaactc cccacgatgc caattggtgg
     2401 gagggtggac agaaggtaaa aagtacccag gagtttgctc ctcgccgcct tgatacgcaa
     2461 cttgatcggc tgacccgcaa acaggcgggt cgtcgttcgc gaactaaaac ggaccgcaag
     2521 cgtggccggt atatccagtc acgccttccg aaaggggaca aggtgcgcga tttggccttt
     2581 gatgccacgc tgcgcgccgc cgctcctcat cagattcgcc gcgatcgctc caagctcgct
     2641 ctggctctgg agaaagaaga ccttcgcgaa aaggtgcgcg ttcgtcgctc ggccaacctc
     2701 atcctctttg tggtcgacgc cagctggagc atggctgtag cggagcgtat ggaggccacc
     2761 aagggcgcga tcatgtctct cctgaccgat gcttaccagc gacgagacag agtggggctg
     2821 atcgtgttcc acaagaatga tgcgaacctg gtattacccc cgaccaactc cgtgcagctg
     2881 gccaaaaagg ctctaataaa tatcgcagtg ggcggcaaaa cgccattatc ggcaggcctg
     2941 tatctggcct atcaggtatt tcgacaggaa gcctacaacc accccgatgt cttgcccttg
     3001 atgatcctgt tgacggacgg ggcggggaat gtcagcatga ccgatatgcc gccccaggac
     3061 gaagcccaca agatcgccga gctaatcagt gaaacggatg tgcgctccgt ggtgattaac
     3121 atggagcacg cggcatttga tcaggggttt gcgcgccagt tggcggagcg cctgggtgcg
     3181 ccctgtcata ccctggagga tctcaaggcg gacacgcttt atcggactgt ccttgaccag
     3241 cttgtgacga attagtcggt taacggttga agttcatcgg cataatgata tgaacgaaat
     3301 cattatcgcc gacgggtttc aggacgccgg gttcattggg gtttgtcgtc tcaagggcga
     3361 cctgtggcgt gtcgatgaca cccaatgcgt cggccatgaa cttgacgttg aaggcgatct
     3421 cgatgggcat cccttcgacg ttggcatcaa cctgggcgac gttatcgccg gtctccttgc
     3481 tgttggcggc aatgatggcg taggccggtg acaggccatc gccgggctcc accgagacgc
     3541 gcgccgtatt gctggattcg cgggcgaaaa tgtcggccgt gcggcaagcc ttggagaaat
     3601 cggccgtatt gagcaccgtg cgcgtattgt ggctgcgggg gatgatcggc tgataatcgg
     3661 ggaatttccc atcgatcagt tgcgagacca gcaagacgtt gtccatatcg aagatgatct
     3721 ggttccgacc gggaggcatg gaaatataca ccgcttccag atcgtcggtg acgatgcggg
     3781 ccacttcgtt cagggcgcgc gccgggatga tgacgttgaa aggtttttcc acataaccgg
     3841 cgatgtgggc tgagcgcacc gacaagcgaa agccgtcggt cgcggccatc gatacccgat
     3901 taccctcgaa ggtagtgagg acgccggtca aggaggggcg agaatcatca gtcgcggccg
     3961 cgaaagccac ctgtgagatc actcgcttca gcaccgccgg ctccatgcgg atgcgatttt
     4021 cctgatcggg ggcggggatg accggaaact cgttggcgtt gatgcctttt acgttcgcct
     4081 gtgtacggcc gcagctcacg tggagggttt cggtcgtctt gttgagggac aactcaacct
     4141 tctcttgcgg gaaagtgttg atcagctcgc taaatgtccg tgcgggcacg gatgttgctc
     4201 ctgcctcgct caccgtcgcc ccaatccagc acgtgatcac gacctccagg tttgtggcgg
     4261 agagcttgag gcggccgtta tctactgcca gaagtacatt gcccaataca ggtagcgtgg
     4321 accgtgggct aacggctcga ccgactatgc ttaatccctt ggcaaggttt tcctggaggc
     4381 aggagatttt catgacacac caccctcatt taattgttgt attgtcccgc atgtttcaac
     4441 cagtatatct tcagtaaacg ggacgcgcaa gaaatgatca ttggcgtatg acgaggaatc
     4501 gtcacgaaaa atcgtccgag taatcgtcgt tagtccgcag tggaatgacg gatgtgatag
     4561 aggactatcg ccagggcaac atggacattg agcgatagcc ctttgccgta catgggaatg
     4621 aagacggccg catcgcaggc ggccagggtg gcttttgtca ccccgtggtc ctcgtggccg
     4681 acgatgaggc acgtcttgtc cggatagttg aattcgtgat aggggactgc gtcctcagtc
     4741 agctcgatcc cgacgatgcc gtatcctttt ccgcgcaacc cttcgatggc cgggagcgga
     4801 tcggcctcat agcgccaggg caggcgcaga ctcttgtatc ggccgacttt gtcgatcgta
     4861 gcattgggtg gtgtcggcgt aatgccggtc aggatcaact cgctcacgcc ggcaccatcg
     4921 gccagccgaa agatggaacc tacgttggcc gggtactcca cactttgcag cagggcgatc
     4981 agatcgccgg ccggtttgtg agcgcgccga taatcccgca ggaatcgttt cagatctgtg
     5041 ccgttcaatg gacgcattgg catatttacg cgacttcctc gaatttcttc agagtgcggc
     5101 gtctgttttc tgcgcttcca acgctcgcaa taaacctgaa attaacaatc agtcacacag
     5161 ccctcggacg ctgacttgac cagcctcaca tacttataga gtgtgccccg cgtgactttc
     5221 agcggcggag cgctccatgc cgcacgccgg gcggcaagtt cctcttcagt gacggccata
     5281 tcgatgatgc gcttttcggc gtcaatcgtc acgatgtcgc catcgcgtac cagagcgacg
     5341 gggccaccct cctgcgcttc gggagtgatg tgccccacaa tgaagccgtg ggatccgccg
     5401 gagaagcggc cgtcggtcag caaggccaca tctttcccca gccccgcgcc catgatggcc
     5461 gaggtgggtg tcagcatttc gggcatgccg gggccgccct tcggcccttc gtagcgtatg
     5521 atcaccacat cccctttttt gatttggcct ttttccaggc ctgccagcat ctcttcttcc
     5581 gcgtcgtaga ctcgcgccgg gccgctgaag cgattgcctt ccttgccggt tattttggcg
     5641 acggatccct ccggcgccaa attgccgcgc aggatttgga tgtgcccggt tgccttgatc
     5701 gggttatcca gggggcgaat aatacgctgt ccttcgctca gacctggaac ttcggccaaa
     5761 ttttcggcga tagtcttgcc cgtcaccgtc aggcaatccc cgttgataaa gccattttcc
     5821 cacagatatt tcatgaggcc cggggtgccg ccgattccct gcatatcttc catgacgaac
     5881 gccccgctcg gctttaaatc agccaggaac gggacccggt cactcactgt ctggaagtcg
     5941 tcgatcgata gatccacatc gaccgatctg gccatcgcga tcaagtgcag gacggcgttg
     6001 gtgctgccac ccagcgccat gacgatcacc atggcgttct caaaggcggc gcgggtcatg
     6061 atgtcgcgcg gtttgatgtc gcgctcgata agcaggcgaa cggccgcggc cgcccgaacg
     6121 cactcttctt tcttttcggc cgattcagcg ggcgtggaag agctataagg caggctcatg
     6181 cccaatgctt ctatcgcgct ggccatggtg ttcgccgtgt acatgccgcc acacgcgccc
     6241 ggccccggac agctatgacg gactattgcg cggcgatcct cctcggtgat ggatccggca
     6301 atcgcctgac catagctctg aaacgcggat acgatgtcca attggggatg ctgtggcgtg
     6361 cagccggcgc gaatcgtgcc gccatagacc ataagcgcgg gccggttgag ccgccccatg
     6421 gcgatcaggc acccgggcat gttcttgtcg caaccgggaa tggcgacgag tgcatcatac
     6481 cagtgtgcgc ccatcgctgt ctcgatcgaa tcggcgatga tctcgcgtga ctggagcgag
     6541 tagctcatcc cgtcattgcc catactgatg ccgtcgctga cgccgatggt gttgaactgc
     6601 atgcctacca ggcctgcatc agccatgccc gatttcaccc aggccgatag ttggttcaga
     6661 tgcatgttgc aggtattgcc ctcataccac atggcggcaa tgcccacctg gggcttgttc
     6721 atatcttctt cggtcagccc ggtgccgtag agcatggcct gggatgcacc ctgggaagcg
     6781 ggttgagtaa tacgtgagct gtatttattg agcataggtg ttatcactcc tctgaggtcg
     6841 tggagattcc tgatatttat atgtgggtgt cgatggccgt tagaagtggc cttcgagctt
     6901 gtctacgacg gcttcggcga tgctcagtga cgaggtggcg gccggggaag gcgcattgat
     6961 gacgttcacg attcgactcg actcgtagaa cgcaaagtcg tccagcaacg ctccatcagg
     7021 ccctaatgcc tgtgcgcgga tccccgccgg cgcttcttcg aggtgttcgc cttgaatgtc
     7081 cgggataagc cgttggagcg ccttgacaaa ggcagccttg cttgccgagc gccatatctc
     7141 cccaagcccc atcttccagt agcgaccggc cagcttgcga aagccgggat agcttagcac
     7201 ttcccaaagt tcgcctgcgt tgatatcgct gagatgatac ccctcgcggg cgaaggccaa
     7261 aacggcattc ggcccacatt ccacgccgcc ggagatcata cgggtgaaat ggacgcccag
     7321 gaaggggaaa ttgggatcag gcacggggta gatcagcgcg ttgcacagat gccaggcttc
     7381 gggcttcaat tcgaagtact cgccacgaaa ggggatgatc ttcgccggcg cttctccggc
     7441 cgacatcttc gtgacgtgat cggactgaag accggcgcaa ttgacgaggt aactggcctt
     7501 aaactccccg gcagtcgtga tgactgtcag tccgtcgccg gcttcattga tttcagtcac
     7561 cttcgcgccg gtgacgatgg aatggcccgc ttcacggagt tgagcggcaa atcgttcaca
     7621 cacctggacg tagtcgacaa tacccgcctc aggcacgtgg atggccttga tcccgttcag
     7681 gacgtgaggc tcgatctcca gcaagcgctc cctgtcgatt atctcgcagt cgacgccatt
     7741 ggctcggccg cgttcaaaca agttattgag cgccggtaac tctttctcat cggtcgcgac
     7801 aatgaccttg ccaaccagac tgtacgggat gccttcttct ctgcaaaact cctccatcat
     7861 ccgtttgccg gtgcggcagg tggtggcttt cagcgagttc ggcttgtaat aaatacctga
     7921 gtgcagcacg cctgagttgc ggccggtttg atggaaggcc agggtttgtt ctttttcaag
     7981 aacggtaata tgtttgttcg ggtatcgatg cgagatcgat cgtgctgttg ccagacccac
     8041 gatcccgcca ccaatcacga tgatatctgt agatgtcata accttaaatt gtaacccgat
     8101 cccctgatgc cgccgatata tgatgggtaa ggatcaggct atccggagcg cagcaatgac
     8161 cctctgtccc atctccttcg tgccgatcgt cggttcgccg gagcgggcaa tgtcggccgt
     8221 tcgcgcgccg tcagccaata cttgagcgac cgcgctttct atggcggccg cttcatctgt
     8281 caggcccagg cttgagcgca acagcatcgc tgcgctcagt attgttgcca atgggttggc
     8341 aatgcctttg ccggcaatat ccggcgcgct gccgtgaata ggttcataga gacctgggcc
     8401 gccgggattg cccagcgagg ccgagggcag catacccagc gatcccgtaa tcatcgaggc
     8461 ctcatcggaa aggatgtcgc cgaacatatt ctcggtgacg atcacgtcaa aatcggcggg
     8521 acgattaatc aggtacatgg cgcaggcatc cacgagaata tgctcgcagg tgatgtcggg
     8581 atagcccgcg gccacttcgt tggcaatttc ccgccacagc cgggatgagg ccaggacgtt
     8641 ggccttatcg actgaggtga ctttcttgcg ccgccctttg gccaattcaa acgccaatgt
     8701 cagcacgcgc cggatttcac tctctttgta tcgcagggtg tcgacggccg ctcgttcacc
     8761 atccaccatc tccggccctt gcggcttgcc gaaatagagg ccgccggtca gctcgcgcac
     8821 gatcatgata tccacgccgc gtacccgttc cggtctcagc gggcttgatt cgatcaaggc
     8881 gtcagcaacg gtcaccggtc gcaggttggc gaagacgccc atcgcttttc ttagccccaa
     8941 cagcccttgt tccggccgaa ccggcgcctt ggggtcgctc cattttggcc cgcccacggc
     9001 gcccagaagt acggcatcgg cggcgaggca agcatccagg gtgttactgg gcagggcgga
     9061 gcctgttgcg tcgatggcgc agccaccgat cagcccctcg acaaatgaaa aggtgtggtt
     9121 ccccgcggca gcgacggctt cgagcacgcg gcgagcctca gcgacgattt ccggcccgat
     9181 tccatcacca ggcagtagtg agattgtagc attcatttat gtattacttc gtgtagttag
     9241 cttgtagata agcagagata tttcgttgag aatgataaag attatgcgtc ggctctcagg
     9301 ttggcgacgg ttccgccggt ggcgtggacc agcttgtccg gcttgatcga gaagaccgtg
     9361 ttcggcgtcc cggcggcggc ccagatcacc tcataatgcg ttaaatcctc atcgatatac
     9421 atagcggggg aaccggtata tccgaagggt ggcacgccgc cgatggcgaa tccggtcgcc
     9481 gcacggacgg tatcggcgtt tgcacgctcc acctgtttgc ggccgacgcc gaatagcgcc
     9541 gccaatttcc gcgtatccag ttgattgctc cccgacacca gggcgacgac aggttccccg
     9601 cgcacggtga atatcaggct cttcacgatc tggccgacgg tgcagccaat ggccgcggcc
     9661 gcgtcttcgg ctgtgcgcgt tgattccttg aaggtgacga tatcgattgt tagccctagt
     9721 gcttcagcgg ccgcggccac gcgttgggcg gaggggtgaa gattgctttc agacatttcc
     9781 aactctagtg aattaaggcg tattccatac tatcggctaa agcccgccaa ctggcgtcga
     9841 tgatattggg gcttgctccg accgtgctcc agcggcgtgt gccctgacgt gtctcgatga
     9901 ggacgcgggt ggttgctgct gtgttgttct caccgtcgag gattctaact ttataatcct
     9961 ccagacgcac cccgctgatt tgaggatata cgtcgacaag cgcctggcgc agggcggcgt
    10021 ccagagcgtt gacggggcca ttcccctcgg ccacggtgtg catgatcttc ggccccacgc
    10081 gcacctttac ggtcgcttcc acgatcgttc cgcgagcgcg tcgccgctgg accatgaccc
    10141 aatagtcggt catctcaaac gggggcacat aggcgggatg cgttcgccgc agcattagct
    10201 cgacggacgc ttcggctccc tcgaaggtaa agccttgcgc ttccaattgc ttaatctgat
    10261 cgagcacctt gggcacatcg atgttgtccg ggttcaattc gtactgcctg atcttgtcga
    10321 ccaggttgcc ccggccggac agttcggaga cgaccgaccg ctgttggtta ccgacctgaa
    10381 caggatcgat gtgctgatag ctggccgcgt gtttgagcat ggcggcgaca tggatgcctc
    10441 ccttgtgggc aaaggcgctc ccgccgacgt agggcaggtg gtcatcgtgg gtaaggttgg
    10501 ccaactcggc cacatagcga gacagatccg tgagttgaac caactgttca gcactcacac
    10561 attccagacc cattttgagt tgcagatcgg gaataatcga gcatagattg gcgttggcca
    10621 cgcgctcgcc gtagccgttg atggtgcctt gcacctggat ggccccggcg cgaacggccg
    10681 ccaacgagtt ggcaacgccg cacccgccat catcatgggt atggatgcca acgggtacat
    10741 cgcccagtcg ctcgatggct tgtctgacgg catgctcgat ctcccagggg agtgagccac
    10801 cgttggtgtc gcagaggacg atgcagtcgg cgtttcctct ggctgctgct tcaagcgtgg
    10861 ccagggcgtg ctcgggatca gccttgtagc cgtcgaagaa atgctccgcg tcgtaaataa
    10921 cttctttgcc caacgacttg caataggcca cgctttcctc gatcatcgcc tggttttcgg
    10981 cgggggttgc ttccagtatt tcaatcacat ggagatccca gctctttccc accagggtga
    11041 cgaccggggt gttggcgccg accagggcct ggatgttggc gtcgtcagct ggacgtgtat
    11101 tttttcgtcg cgtgctgccg aatgccgcca gcctggcgtg agacagcccc agcgatggcg
    11161 cgcgccgaaa gaattcgaca tcttttgggt ttgagccagg ccatcctccc tcgatgtagt
    11221 gcatgccgaa agcatcgagc cgcgcggcga tcttcagctt gtcgtccagc gatagtgaga
    11281 taccttcccg ctgggttcca tctctcaacg ttgtgtcata gaggtatata gtcatgataa
    11341 tggatgagcg agggtgttga ccctggtccc gtgcccggtt tcataggctt cgatttcagg
    11401 cttgtgctta aggagatagc ccaactggtc cagcccttcc agaagacagg tcttgttgaa
    11461 ggcgtcgatt gggaattgaa cccggcggcc gtcggggaga atcagggatt gggtatccag
    11521 atcgatcgat agttccaaat ccggctcttc ggcggccagg ctcattaatt gccggtgcgt
    11581 gtcgtcgtcc acgaccagcg gtaacagccc gttttttagg gcgttattgc gaaagatgtc
    11641 ggcaaatgat gtactgatga tcgcgcgaaa tccaaagtcg gtcaaagccc atggggcgtg
    11701 ctcgcgactg gagccgcagc cgaaattgtc gcccgctagc aggatttgcg ctccctcgtg
    11761 ttgcggctgg ttgagcggaa aatcgcggtt gggcgcgccg tcgctgtcat accgccagtc
    11821 ggcgaagaga ttcttgccca ggcccgtctt gctgatcgtc ttgaggaatc gcgccgggat
    11881 aatctggtca gtgtcaatat gatcggccgg gatggcgacc acctgagcgg ttagtttgtt
    11941 gaatggttcc ataatgatcc gaagtcgttg aacgatagtt gaacgctagt taaacgataa
    12001 caacaggcag tgaaagatgc cgtgcaatat taggcgaact cgcgcacgtc agtgatgacg
    12061 ccggtcacgg cggtggccgc ggccgtcaac gggctggcca ggaaggtgcg cccacccttg
    12121 ccctgacgcc cctcgaaatt gcggttgctg gtactgacgg cgtactgtcc gggagatagc
    12181 tggtcgccgt tcatggcgat gcacatgctg cacccggcct cgcgccattc ggctcccgcg
    12241 gccttgaata tgcgatcaag cccttcggct tcggcctggc gcttgacgtc ctgtgaaccg
    12301 ggcacgacca tgacgcgcac cccatccttg actgagcgcc catccatcaa ccgcgccgcc
    12361 tcacgcaaat cgctgatgcg cgaattggtg caactgccga taaatacaac atcaaccgga
    12421 tggcccagca gggggcgacc cggttccaga ctcatataat cgagcgcccg ttgcagggtc
    12481 agccgctggg tgaggtcccg gatgtcttcg gggttgggga cgggcgccga aatggggatg
    12541 cccatgccgg ggttcgtgcc ataggtgatc atcggctcca gcgcgccggc atcgatgtcg
    12601 accgatttat catacaatgc accttcgtcg gttggtaatt ggcgccagtt tgcgacggcc
    12661 gcatcccagt cggccccctg tggagcgaag ggccgcccat gcaggtagtc aaacgtcgta
    12721 tcgtccggcg cgaccagccc tgcccgcgct ccgccctcga tgctcatatt gcagatggtc
    12781 atccgctctt ccatcgacaa gccacgtatg ccggagcctc tatattcgaa gatatggcct
    12841 gtgccgccac ccacgccgat cttcgccagc agggcgagga tgatgtcctt ggcggtcaca
    12901 ttgggcttta gttggccggt gacgttcaca gcgtaggtct tgggtttcac ctggagcagg
    12961 cattgagttg ccaggacgtg ttcgacctcg gacgtgccaa tcccaaatgc cagagcgcca
    13021 aatgcgccat gggtcgcggt atggctgtcg ccgcagacga tggtcatgcc cggttgcgtc
    13081 aaccccaact cggggccgat cacgtggacg atgccgcggt ttgggctgtg catgcccagg
    13141 agctggatgc cgaaatcacg ggcatttttc tccatctggg cgatttgggc cgcggccatc
    13201 tgatcgaaaa taggtacgtc gaggggggtt gtgggcacgc tgtggtcgat cgtcgccaca
    13261 gtctgttgcg gccgacgcac gacgagcccc cgctctcgca ggccggagaa ggcctgggga
    13321 gacgtcactt catgaatgag atgcagatcg atatagagga cgaccggtga gttgggttgc
    13381 tgggctacga tgtggttgtc ccatatcttg tcgatgatcg ttctgggttt aaggtcagtc
    13441 atttattggt tccaacaaaa ttgttcaaaa tttccgtcat ggcggcgaaa gaaaccacgc
    13501 tcaggaggat gccggtttca tgccgccgaa taatgccagc tcgaatgcct tgaagtagca
    13561 gtctacgtgt tcatagccga tttctctcag ccaatcacac tgcaattcca ctggagcaag
    13621 gatattggcg ctcttgtcgg ggcgatagta atactccgcg gccacttcgt cgcgcgtctt
    13681 tccgtttggg gcatgatggc gatacagggc atcgatgaag tattcctcga attgctcctc
    13741 gacccagcgg gtggccgaag cgacgtgttc gaggttgagg aacaggccgc ccggcgatag
    13801 aagagcatat atctctgcgt agatttcgcg tttgcgttcg tccggctgat gatgaatcga
    13861 gaagccggac acgatcacgt cgaatccgtc gctgccgttc ggtaagcttt tggcgacaac
    13921 ctcggcccaa tccggttggg cgtaatctat ctcaaggaat agtccatcgt aaccgctcag
    13981 gcgatcccgg gcgacttcca gcattcgcgg cgagaaatcg gcaaacacgg ccgtatccac
    14041 cttgccggca taggcatcca gcagcgcgtg ccccagaatg ccgtcgccgc aacccagatc
    14101 gagcaaggcg gtggctgccg gctgggccgc cagcgccaac ctgatcagga tgtcgatctg
    14161 tgcggatgcg aacggtatcg cgccacgcac accatcaaga taggtgtctg ccagcccgat
    14221 cgcttgccag actgtatcct gtcgcgggtc agtcactatt ttgctgcccc gttggtcgcc
    14281 ggctggagcg tgtccacata gccgtcgcgc tcggcgagca tcttgttgag cgcacttagg
    14341 taagcgcggg cgctggcaac gacgatgtcg gtgctggccc cgtgtccgct gaaggtacga
    14401 tataccggct tatcggtctg tgggtttacg gtgaagtctg tcccactgtc cggttggatg
    14461 cgaatggtcg cttcggccac tgcatcaagc ccctcggtga cggcattgat gctgaactcc
    14521 gtcaggcggt tcgtgacgcc gatgatacga ttgatcgcct gatacaccgc atctacgggg
    14581 ccgctgccta gcgcggcatc ctggtattgc tccccatccg gcccgatcat gacgacgctg
    14641 gcggtgggga tgctgtggtc accacaggaa acctggatat gctcgagctg ccagatgacc
    14701 ggcggctgat agatctcatc ggcgataatc gcctcgatat cggcatccgt gacgaccttt
    14761 ttcttgtcgg ccagcctctt gaagcgtttg aaggcggccg ccacttcgtc atcatccata
    14821 ttatcgtagc ccatctcgtg caaatgggcg cggaaggcat gccggccgga atgcttgccc
    14881 aagacgagct tcgaggtgtt gaggccaacg acttccggcc gcatgatctc ataggtctgg
    14941 tggtttttca gcatcccgtc ctggtggatt cccgcctcgt gggcgaaggc gttggcgccg
    15001 acgatggctt tattgggttg gatgaccatg ccggtatagg tgctgaccat gtggctcgtg
    15061 cgcgcgattt gggtcgtgtc gatgttggtg gtcaggttga agttctgcgg ccgggtggtg
    15121 agggccatga ccacctcttc cagggctgtg ttgcctgccc gctcgccgat gccgttgagg
    15181 gtgacttccg cctggcgcgc gcccgcctga ataccggcca gcgtattggc ggtcgccaat
    15241 cccagatcgt tgtggcaatg gaccgaccag actaccttgt ccgatcccgg tgtgttgcgg
    15301 ataagataat ggatgagatt gccgaactca tcaggcgttg tgtagcctac cgtgtcgggg
    15361 atgttgagcg tggttgcgcc ggccttaatg gcctcgccga ggacctggac gagaaactcc
    15421 gggtccgaac gcccggcgtc ttccggcgag aactccacgt cgtcacacag cgaacgagcg
    15481 aacgaaacag ccttgatgac ctgatcgatg cattcttctc gatcaatgcg aagcttgtgc
    15541 ttcagatgaa tgtcgcttgt cgccaggaag gtatggatac ggtgtcgggg cgccgcctta
    15601 accgcgtcat acgcgcggct gatgtcttcc ttgtttgcgc gtgccagtcc cgcgatgacc
    15661 atcggccgcc catccttacg cccctccacc agcgagccga cctcttcggc aattcgacga
    15721 accgcctcga agtcaccggg cgaagcaatt gggaacccgg cttcgcagat atcgacgcca
    15781 agtcgagaca actgtcgcgc gatttccagt ttttcctcta cgttaagcgt cgcgccgggg
    15841 gattgttcac catcacgcaa agtggtgtcg aaaatcagga tggaatcggt cgttggcatt
    15901 tgttgggtca tgtacacacc tttatgatcc ggtagggggc gaggcctgct atcaaagctc
    15961 cacaccccca cgcttgtcgt cgatacttag acctctttgg cgttcagcca gggcatcatc
    16021 ttgcgcagtt cgcggccgac ttgttcaacc tggccattgc gggcttcctc gcggcgctga
    16081 ttgaaccagg gacggccctg ggcgttttca tcgatccacc cttcggcgta ggctccggac
    16141 tggatatcgg ccaggatttc cttcatggct ttccgggtct gatctgtgac gattcgcggc
    16201 cccgccgaat aatcaccgtg ttcggccgtg tcgctgacgc tatagcgcat gtaattgagg
    16261 ccaccctgat agatcaggtc tacgatcagc ttcaactcgt gcaaacattc gaagtaagca
    16321 atttccggtt gatagcctgc ctcaaccagc gtattgaatg ccgactcgat cagatggctg
    16381 acaccgccac acaagatgac ctgctcgccg aacagatcgg tctccgtctc ttccgtgaat
    16441 gtggtctcga tgacgccggc gcgcgtgcag ccgatgcctt tggcgtaggc cagggcagtg
    16501 gctgcggcgt taccggtagc atcctgatgg actgccatca ggccgggcgt ccccacgcca
    16561 tcgacgtaca actcacgcac acggtggccc ggcgacttcg gcgcgaccag gctcacatct
    16621 acgcctgccg gtgggacaat ctgcgcatag cgaatgttga acccgtggcc aaacatcagg
    16681 gttttccctg gtgacagatg aggcgcaata gcctcgcgat aaatcctggc ctgggaggtg
    16741 tccggggcga ggatcatgat gatatcagcc tcggctgccg ctttggccga tgatttcaca
    16801 accaggccct gagcctcagc cttggcccat gatggactgc cttcgtaaag gccaacgcgc
    16861 acatccattc cggagtcgcg cagattaaga gcatgagcat ggccctggct gccatagccg
    16921 atgatggcga tcttcttgtt ttgaatgacg gacagatcgg cgtcttgatc gtaataaact
    16981 gtagccaacg tgataactcc ctgatttgat ttcaagaaag aaaccgggtc tcctctgtgc
    17041 gattcccggc ttcaaatgtc taaatttaat ctctgtgacc gtttcgctct gctacgaccg
    17101 gaacggtggc agaaggcgcg gttgtccact cgcttgcgtt gtgactgtga ccatgggaag
    17161 aactacctcg taccatggcg actcgtccgg tgcgaaccat ctcctcgatt ccgaagtttt
    17221 ccagcaatcc gatcatcgaa ttgacgcggt cttccggggc gacgatctga atgatcaccg
    17281 aggacatatc cacgtcgacg atgctggcgc ggtatatttc gaccagctgc atcacttcgc
    17341 cgcgtgtttg gcttgtcgtc ctgaccttga cgaaggccat ctcgcggacg acagccggct
    17401 cattggtgac atcctcgata tggacgacat tgatcaactt gtcgagttgc gtcgtgatct
    17461 gctgaagatc tcgttcggtg ccgcgggcga cgaaggtcat gcggctaatg cctgccgttt
    17521 cggtgtggcc aacattgagg ctctcaatat tgaagttccg ccggctaaac aagccggtca
    17581 cgcggttcag gacgctgggc ttgtcttcca gccacgcgat tatggtatga cgtcgaattt
    17641 gtagatcgga catgataatt agctccctag ttttcaaccg gccggccacg acggatcatg
    17701 tccgcattgc tcttgcccgg cgcgaccatt ggatagacgt tggtgaattc ctcaacctgg
    17761 aagtccagca gatatgggcc gtcggtttcc tgggcatcgc gaatggcctg atagacatct
    17821 tccttgctgg tgacggcgcg ggcaggaatc ccgtaggctt cggcgatctt cacaaagtcg
    17881 ggattgtaca aaggcgttcc cacataacgc ttgttataga aaacgtcctg ccattgacgc
    17941 accatgccca aatagccatt gttgatgatg gcgatcttga taggcagtcg ttcctgcatg
    18001 atagtcgaaa gctcgatcat ggtcatctga aagccgccat ctccggcaac aacccacatc
    18061 tcttcctggg ggttgccgat ttgcgcgccg atcgccgcag gcaaggaata tcccatagtc
    18121 cccaaaccgc ctgaagtcag caaactgcgc ggcttggtgt ggttgaagta ctgggcctcc
    18181 cacatctggt gctgccctac atcggtgatg atggtggcgt ccccctgatt ggtggcatac
    18241 cacaattgac gcatgacata tggggcgacc agttcgtcgg tctcctgggt cagaatgtcc
    18301 cgttcgacag actctgaacg ccattcgttg atctgcccca gccaggcggg atgctcgttt
    18361 ggctctacct tgggcaggag ggcgcgcaat gtcatcagcg cgtcggcgac gacaggcgta
    18421 tcaatcgtca ggttcttgcc catttcggcc gggtccagat cgacatggat gatccgggcc
    18481 ttgggggcga acgtgctgaa ggctcccgtt aggcgatcgt cgaggcgcgc cccgatggcg
    18541 aacatgacgt cgcactcctg caaggcgtag ttgcaatacg cttcaccatg catgccgccc
    18601 caactgatcg acaggggatg ggtagcgggt atcgccccga tccccagcaa gcttgtggca
    18661 accgggatgt tggccttttc agcgagttgc cgcagttcgg cctcggcgcg agccatcgaa
    18721 actccctggc cggcgacgat gacggggcgc tgggcttcgt tgatcagccg ggcaacgcga
    18781 tcgatgactc ctggatccgg cgcgaggatg tttggccgat agccgggaag gtagacctcg
    18841 gttggatagg tgaactccat cgtggcctgc tgcacatcct tgcagatatc gacgaggacg
    18901 gggccgggcc gtccggtgcg ggcgatgtag aaggcttcct tcaaggcggc aggcaggtcc
    18961 ttgatgtcgg tgatcagata attgtgtttg gtcacgggca tgctgatggc gcggatatcc
    19021 gcttcctgga agccgtcctt gcccagcagg gcggtggata cttgcccggt gattgctacg
    19081 accggggttg aatccatata cgccgtggca agtccggtga tcaaattggt cgcgcctggg
    19141 cctgaagtgg caatgcatac acctgtcttg ccggtggccc gggcatatcc gtcggccatg
    19201 tgcgccgcgc cctgctcgtg agtcacaagc acatggtgga tcggataatc gtacttatac
    19261 agatcatcat aggtcgggag aatcgcgcca cccggcaggc cgaagacgac atcgactccc
    19321 tcgtgaatca ggctctccca tatgatctgc ccgccggtct ttagttcgct cattttcttc
    19381 tcctcaatga aaaatactaa acaaaaaacc gccctgttgg gcggttttcg tttgagcttt
    19441 atttgtgttc ccggttcgtg ctctttaacc tctacccaac atgcgacgta tccttattaa
    19501 taataaggat aaggccaata aggtcgagga cgagtacgtt ctggatgtaa ggtatcattt
    19561 ctttgccatt gatggcttga tttcctgaag tataaggtca ggtcccaaat tggggctatg
    19621 tataaataga caaaacatga ggccgatatt ttgtgcaaat tgaccaaatg ctcgcctgga
    19681 taatcgaacg caaagcagcc aaaaaccaaa gggaacgacc tcttgtgaaa gtcgttccct
    19741 tcagttccca ctatttccca ctgaaccgcg cactctagcg caagccttcg aagagtatat
    19801 agcaggcaag agccggatca tacggttata gtggcagaat tggagcgtga ttattttgta
    19861 cactattcac aaatgcaggc atcaggcgga tggctgatcg ctatcaggtc gcaattgcca
    19921 caacttcaac gaaagatgaa gggcaagtcg catattggca tggtcgagat tgagccccgt
    19981 cagctcttca atccgctcca tccgatagag cagggtgttc cgatggacga agagcgattc
    20041 ggccgtctga ctgatattgc catgatgatc gaaatacgct tcgaccgttt tgacaaggct
    20101 tccccgattc tgattgtcat attcgattaa cggctgcatg atttcgtccg cgaattcgcg
    20161 aacttcaggt tgttcttcca gggtatagag caaccggtaa atgcccaggc tgtcgaactc
    20221 aacgaactgg ttggtcaact taagacgttc gcccaactgc atggcttgca aggcctgatc
    20281 atacgctgcc ggccactcgg agagcgtttt ggccgggccg ctgatgccgc caatcagtgt
    20341 tgcctccggg tattccatct cgatttgttc gcggatgcgg cggccgagtt gatgggcgct
    20401 atccatgtcc tccgggttct tcaaggcctg aaaaatcacg agatactgat tgccgtagat
    20461 atgaaccaaa gccggtcgtg gatggttgga aagaacccaa tgggttgaag tctcaagtcg
    20521 tcgcaagctg agcttattgg ctccaatcca gccggcgacc atgacggcgt gcggctcgga
    20581 cgtgttatga tccagtcgcc cctcaagccg ctcaatttcc ttgcgtggca gcgaaccggc
    20641 cagcaatccc tcaagaaaat caccgcgcaa cgatttcttg gcttcgttaa cagccttggc
    20701 tttggccatc tccagcgcgc aggcggccgc gccgtgctcg acggttaaac tgtcgaacat
    20761 atcgagttcc gccactgggc caatgaccga taggtaacca cgcgctcgat caccggagat
    20821 gatcgggcta agcaatcgcc ccagatgaac agatgaatcg ccgagtggga gtaattgttg
    20881 ccagatgctt tgtcgcgatt tggcggccgc cttgcggttc ctcaggacag gaggcagcgc
    20941 atctctctgt tgcagcgcct caacgagtgc ttcgcggttg acgatagtat tgctcggcca
    21001 actaatggcc tgaatctcaa gtcgcttatc ctggacgacg acgatattgc cggttagctt
    21061 gctcatcacc tccgtcatgg ccgccaggcc ttgaccctcg cgagacatgg cggatagctg
    21121 gcgatagagc tgaatcgccc gttcgctcgt ggctgtttgc cggtcgagaa gcaggacgga
    21181 gacgttgtga tgtatttctc gaactgatgc attttccggc gcgatcagca cggtgaggtt
    21241 gtgggcttcg gcccggtgcg caagttcatt gctaaaacaa accagcccgg ctatgggtag
    21301 attggctaat acttcgagtg ctgtggtgag tttgcctgaa tccgggactt cagagagatt
    21361 gggcggcacg atcacaagat catgggggtc gatttgtgat tccaggtcct cccaacttgc
    21421 cagtatgacg acccaattga cgaatcgcct ggcctctgcg ctgccgccaa tcagggttgt
    21481 atcgatcggc aaagccaatc gcagcacatc cccaatattt atacccgcag gaggcgaatc
    21541 ggtgcgcatt attttctcgg ctcgtgatat ggacggggat ggctcatgtg tggaggtagc
    21601 catgatgaat gcgtgttggg caggatagaa atcttgtatc gggtgagagc ttatttgata
    21661 tagaatccgg aggcgccggc gtcaggacga tgcacgggca gggtgatgaa aaaggtcgtt
    21721 ccctcgccta atgcgctgga cacggtgatc tccccgccat gttgttccac aattcgatgg
    21781 caggttgcca accccagccc tgtgccctgg cccggtcctt tggtggtata gaatggctcg
    21841 aagatgtgca gcgactcggc cgagttcatg cctttgccgt tatcgcgaag taacacctga
    21901 acggactggc cgtccttgag agggcgagcg atcacgtcca gccgtcggtt gtcattggta
    21961 tggagtagcg cgtcgcgggc gttcagcaac agattgagcc agacgctctt gagatgttcc
    22021 caactggcaa ccatctgcgg caaggaagca tcgatatcgc gattgacggt aattttcgat
    22081 gattgcaatt gatagttgac aagggcaagg gcttcctgga tcgatttacc aagatccccc
    22141 gtggtgaatg aatattgttc ctggcgagca aaatccaata gaccgcgtac aacctgagcc
    22201 gcccgctcgc ctgcccgcac gataaggtcc actgattcaa aattttcgtc ttccaccggc
    22261 ataaccatct tcagcatttg agcattggcg ttgatggccg tcagggggtt gttgatttcg
    22321 tgggcgacgc cggccgccaa ttgtccaata gctgccagct tcccggcttg catcagggaa
    22381 ttctcgagac gtcgttcttc ggtcttgtct tgccagacga cgacagcgcg cgccgaactg
    22441 gcttgtttgc ccggtatcgg ataggcgctg acttcccact ccctggggag atggtcctct
    22501 ccttgccagc ggacgggcca atgttgttcg gttttgtcgt tcagggtgaa tgcgactttg
    22561 caatggtcac acggtttttg ccggttataa aagacccgga aacacgtgcg ccccgtgaat
    22621 gattcaggtg tggaacccaa ctcgtcagcc ttcgccttat tgatgacgac gagtttccat
    22681 tcatcattga tcgtatagat agggttcgga atgccgtcga tcagagcctg taatgtgttg
    22741 cgcccctcga acaactcctg atgccgcagc cgcacgcgct ggaacaacca tgcattttcg
    22801 attgcttcgc tgacggagct tgcaagcgaa gagaagaggt cgatgtcgac atcggtgaac
    22861 tctcctgaac gcttgttata cgcggccagc acgccaaccg atcggccgcg cacggccaac
    22921 ggcgtagcca acatggagcg cacgggaatg ccaatcgggg agtcgcgcgt gggatcatat
    22981 tgctcatcct cagcgacatt gttcaggcat agagtgacct ggtcgctcca ggcgcgcccc
    23041 agaacgccgg ctcctttttc ggttgagggg aagatgtgcg aaggaattgc tcccttgtag
    23101 tggcggacat gctggcaatg caatcgaccg ccttcaccga tcaagacggc cgccatctcc
    23161 acttgcagga tggcatccac gccgcgaata gcggtggcgt atacctggcc ggtttcgagg
    23221 gtcgcggcga cagcttgact aatgcggttg agttcggcaa gctgccggct ttgctggtgg
    23281 agttgtttct cgagctgctg ggtgcgcaag atcgtcttca cccgcgccaa caactcctgc
    23341 atgtaatagg gtttggtgat gtagtcgtcg gcccccgcag agagagcttg caccttttcg
    23401 tcgttgctgg ctgcggccgt taatagaacg acgcgggtat agcgcagatc gggatggccg
    23461 cgaatccaat cgagcacctc aaggcccgat atgccgggca tccgcatatc gagaagtacc
    23521 agatcaatct cggccaatcc gccgattgac ttctcgagaa acgcgatcgc ttcctggcca
    23581 tcatggacga tatcgacctg gaaaccttcc ttgcggatga ggaattcggc cagggaatcg
    23641 gcgatttcaa tttcgtcatc gactacgagg atacgagcgg tcgactggga cgacatcgac
    23701 acaggagaag cacccccggc tggacgccct tttgggagtc cggtcgcagt tcctcctgtg
    23761 ccgttatccg cccgcttctg agcagaaggc ccaatgagcc gctcctctac ctcagtgccg
    23821 atagtcttat ccacgcgttt cctcagaaga gaaggagtac ccgtgtccct gtatcgtctt
    23881 gatgtacatt ggatcggcgg gacgaggctc gatcttttcg cgcaaattct tgatgtgggc
    23941 acgcacaagc tcgggactgc ccgtatctcg cgggtagtcc cacacgtctt gcagaagttg
    24001 ctgactgtta aacacctgat cggcgttgct catcagatgg tagagcaggt cgaactgaac
    24061 gtttgtcagc aagatggtct ggtatggcgt cgtcaccttg aacgtgcggc aatcgagaac
    24121 aacgttgccg acgaccacct cattgattgt attatcctca atcttttcgg ggccaatgcg
    24181 tcgcaggatc gctttgacgc gcagctcaag ctcctgcata ttgaacggct tgacgagata
    24241 atcgtcgccc cctgcgcgga agccttcgat cttgtcctca tctttcgctt tggcggtcag
    24301 gaaaaggaca ggcacatcct ggagcaatgg atcgccgcga atctgtcggc aaacctgata
    24361 cccgtcgacg ccaggcatca tgatatccag gataaacagg tcaggccggt ggcgtcgagc
    24421 caactgcaag ccttcgatgc cgctattggc ggtcatcacc tgatagtcat acatcttcaa
    24481 tgcgcgctgt aatgttcgcg agacgagatc atcgtcatcg atcacaagga tcagggccat
    24541 aaacttctac cccttctttg tccaggattt tgcctatcag tataacctaa cacaccgaac
    24601 tcgtcagtta ttgg
//
LOCUS       DAOMQG010000015        24223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_899_length_24223_cov_6.212388, whole genome shotgun sequence.
ACCESSION   DAOMQG010000015 DAOMQG010000000
VERSION     DAOMQG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 24223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24223
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_899_length_24223_cov_6.212388"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..3285
                     /locus_tag="PLC06_02010"
     CDS             112..3285
                     /locus_tag="PLC06_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRO89249.1"
                     /translation="MPSSDEQRAQLEAGIAALEAQRATLGDEMVEAILGPAREKLAAL
                     ESRSQSQQQRKQATVLFADVSGFTAMSETMDAEVVAGVMNELWAEVDRAVTDHEGRID
                     KHIGDEVMALWGADTAREDDPERAVRAALAMQAAVARFCETRGMTLAMRVGINTGPVV
                     LGAMGTTAEFTAMGDAVNVASRLEHAAPVGGILIGHDTYSQVRGIFDVLPQDPLSIKG
                     KAEPVRTYVVQRAKPRAFRMATRGVEGVETRMIGRDAELETLKNTYLDVMESAETRMA
                     VVVGEAGVGKSRLLYEFENWIEMRPEMIWYFKGRATSNAQNVAYSLFRDLFANRFEIL
                     ESDPIATALDKFRRGMAGALEPDSADIVGYWLGFDFSGSEAVQGLLGDSGLMQSARAF
                     LMRYFRALTADSPTVIFLEDVHWADDSSLDLAVYLATALPEARLLLVAVTRSRLFERR
                     PNWDEGRTVFRRISLMPLPKRSSRALVDEILKRVDEVPDSLRDLITDAAEGNPFYVEE
                     MVKMLIEQGIIERGGAVGIGSGDDSLSPAPLLPGSPAAEEKWRVRADKLATLKVPPTL
                     TALLQARLDGLPRLEREALQRASVVGRLFWDDAVADLLEVERETVTPALEAARGRELI
                     FRRERSAFAGTNEYIFKHVLLRDVAYETVLLNRRAAYHGRAARWLETHAGERLSEYLT
                     LIAEHYIQAGEGLRAAALLERAGDEAMQAGANLVAQTTLERALALREAAGERDGPAVD
                     AALISIGRASRTLGDYAAAKAALERGLVGARSSGNRAREAEALGGLAYNARVQGDYSH
                     ALALVEEALRLGRPLGGRLLLILLDTAAQVLSAMGDLDGAESRAMEALTASRTDGDLA
                     GERMILFTFGAVAAERHELERSKEYFLLSLEAARRTNHLYGEGLALLGLGELAYRLGD
                     YATARTYGRQAEERVREMDMPVHIMNAFGNVAQAELKLGDVAAARRGASEALQLARSL
                     GALPDVLWAVFLHGQIMAEAGEKARGLLLFGLARAHPALDYQVMREINEEIARLGLPP
                     EEVEAGLAAGAALDLETVVGEIMDGKW"
     gene            3329..3547
                     /locus_tag="PLC06_02015"
     CDS             3329..3547
                     /locus_tag="PLC06_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89250.1"
                     /translation="MTPYELVYSMMTPFLPVLYGKVRRDVRSFSPRRPLTTAKAMAGN
                     LINRWESRGLDEQPSRTAHLFAIARKEK"
     gene            3548..4489
                     /locus_tag="PLC06_02020"
     CDS             3548..4489
                     /locus_tag="PLC06_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89251.1"
                     /translation="MNANLVRPFRPTTDLIPLVDLVNAVETAEGREPSLTVERLESML
                     DVPGLYRWVAVAPDAVAPDDPGRLAGYGVVFHQMPQRCYGDVRVHPARRREGIGRRLI
                     DPMVQKATDLGARYLTIDVDAANQDGLRFLLSQGFRFRGDVWALVAPAAVALPPPLWP
                     AGYSVATYADSPDLPGYVELCNRTFGDLWGHWENFPGAMDETHLTGILEQFDPAGIFI
                     VRDSAGKAVAQCRAKAAADNTSPHILDQPGVIPAARDAGLHAPLALTAAHWLRAQGAQ
                     PVRLESWGDSAATVAIYEALGFHQTGHELSYARELGG"
     gene            4563..5171
                     /locus_tag="PLC06_02025"
     CDS             4563..5171
                     /locus_tag="PLC06_02025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89252.1"
                     /translation="MITHLLPPQLDAATATLTAAFDADPIYTYAFPAAKDRARYLPPF
                     WRAILRNAMLHGHVYTTAEADGVALWMPPGRHPVAFGDSARIGFAFHRAVAMFPAAAR
                     RRFMAYVTYGDGVQQRLLGDSLYWELWVLGIRPERQGQGLGGQLLAPILNRADDERFP
                     CYLLTESERNVAFYRKHGFDVAHEGDVPGHPLRLWMMIRQPR"
     gene            5448..6887
                     /gene="proS"
                     /locus_tag="PLC06_02030"
     CDS             5448..6887
                     /gene="proS"
                     /locus_tag="PLC06_02030"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRO89253.1"
                     /translation="MANKTFPGRNEDFSEWYNQLVLQSEMADYAPVRGCMVVRPYGWA
                     LWENISSWLDAEFKKTGHVNAAFPTLIPLSFFEKEKEHVEGFSPQLAIVTHGGGEELE
                     EKLVVRPTSETMFGYMYSKWIKSWRDLPVLIVQWGSVLRWELRTKLFLRTAEFYWHEG
                     HTAHATAEEAQEEMLRILDIYEKFSLEEAAIPVIKGRKTDSERFPGAQITSSIEAMMW
                     DRRALQAGTSHYFGPNFAKAFDIQYLDKSNTLQYCETTSWAISSRMIGAIIMVHGDDQ
                     GLVLPPRLAPIQVVIVPIFKSDAEQAAVMEAADRIFAELKAAGVRVKLDDRDNVSPGF
                     KFNDWEMRGVPVRIEIGPRDVENKSVALARRDRLGKEGKSFIPQANLVVEVEQLLVAI
                     QSSLLQRATEYRDAHIHEPANYDEFRQIIADGWAYAWWCGRAECEAKIKEETRATTRC
                     IPANQPDEHGACIYCGRPASERVYFARAY"
     gene            complement(6956..8719)
                     /locus_tag="PLC06_02035"
     CDS             complement(6956..8719)
                     /locus_tag="PLC06_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89254.1"
                     /translation="MKGQSIIRKRIIRRALPVLLALLALSVAGIVAAHPLGNFTISRY
                     SALTLRAAAVDMLYIIDMAEIPTFQERQAMDADGDGTASAAEEDAWVEQTIADLAANL
                     RLTVDGAAVALSPTSHALSFPPGQGNLETLRLEISLSAALPAVDGDAPRALAYEDGNF
                     AGRPGWQEVIATAADGSLLDSSVPAADLSDQLRNYPADLLTAPSAVNRAEMRYAPMDG
                     ATTGDRQPTTMGGQQAAAAANRFGQDEFANLLNRTLDTPGALPAVLLLAVALGAAHAL
                     TPGHGKTIVGAYLVGSRGKARHAVFLGLITTITHTAGVFALGFLVLAASEFILPEKLY
                     PWLGVLSGLLVVFIGASILRGHLRNQLALRRGLAGADEQYHFHFGKGHSHGPDDHHHG
                     PHSHDHHDHGHAHAHGRQQATSSANAAVTMAPARSLAFAGAMQPAAVAPSPLVAHDHD
                     HDHAHDHSHTHDHEHQADQSPLPTDHAAEPLSRRNLLALGISGGILPCPSALILMLSA
                     IALHQVGLGIVLIVAFSVGLAGVLTGIGLFMVYAGQLLERLPIRHTAMTTRLLPIASA
                     TFITVAGLLITLKALGEAGVI"
     gene            complement(8758..10101)
                     /locus_tag="PLC06_02040"
     CDS             complement(8758..10101)
                     /locus_tag="PLC06_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO89255.1"
                     /translation="MSTVSENRKLTLLVLFTALILIASYALRAGSNFTGVPALLEMVL
                     GPGEPPIPDIMSPAQQVDFWTNRLGPNTQDYITMTHLGQAYLQEARETGDAALYQRAE
                     ESFRKALEFNPKYEPAKALIGSVLIGQHAFADALTFAQAALATNPDEYQALAVAGDAN
                     LELGSYPAAEEAYRHLLQKYPGGAIYSRMARLVWLQGRPDEAIDWMKKAADETLALDI
                     GGEELAWYRFQLGELYFNTGDLRNAEKWYHEADQAFPGHYLAQSGLGKIEAARGHLDE
                     AIARYTTLVARLPQPNFVAFLGDLYALQGDTAAAQAQYDTVLFIQQLDVAQQVLYNRQ
                     MAVFFANHALQADTALAYAEAELAARQDIYAYDTLAWALYRAGRYDEARVASDKALAL
                     GTQDALLFYHAGMIAAARGDVAAAREYLGRALDLNPWFDLLQAQTARETLDGLEG"
     gene            complement(10213..11265)
                     /locus_tag="PLC06_02045"
     CDS             complement(10213..11265)
                     /locus_tag="PLC06_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HRO89256.1"
                     /translation="MNKVRTFIVMAVVMLAMVLGLAALGAGAADHLDAPIVKQDGRLD
                     INDVYAFVHGDNTVLVMTVNPAAGVLSPTTLRSTPGTRYEFAIDNDGDYMEDAVLKLR
                     ADRPQKSGRQRVQLYWQDVDAGTQRRIGAGLSDKNLPLRTGGWFYVGLRDDPFFFDLQ
                     AFRDQIGGAGGSRTFCDGNEVDFFAGLNGTAIVVEIPSDLLDSDDGMINVWARTVNRM
                     TVVDRMGKPALNTVFIDAADKDAYNATTPANDPAMWGDRFKAVLGLFGGYDDATAGVI
                     TGLLLPDVLTLDTNSTNGFVGTLNGRQLAEDVIDFELTVVTGGLIGAPGIPGDCVNMN
                     DQPFMTNFPYLAEPNS"
     gene            complement(11620..12522)
                     /locus_tag="PLC06_02050"
     CDS             complement(11620..12522)
                     /locus_tag="PLC06_02050"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRO89257.1"
                     /translation="MERNQRIEEELFPFYALDALTEEERAEVDGYVAGNPAAAARLAV
                     LMRDAADLTMATTPPIAPAPAVKAGLMARVAADARPATPQVAAAAPPPRPAAEPRPAA
                     ARRSWRDWFTSGRPAMALTAFALVALIVSAAAVARLSRQNAAQQEQIAALQADDAGLR
                     AQLDALTNENEVLRRGLAEREDLLAQFRHPGMRTVAIGDATGDHPAAVGTLTTDMATG
                     ETTLRVSNLPLPSSGDTYQAWVIVGETPISAGMFVVDANGNGTHILSGELPPEFDAIG
                     VSMEPEGGSATPTQGKIILLGRML"
     gene            complement(12526..13101)
                     /locus_tag="PLC06_02055"
     CDS             complement(12526..13101)
                     /locus_tag="PLC06_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO89258.1"
                     /translation="MDQMTGTSDEELMARVTARDPDAFERLYDRYAPITLGIIVRIVQ
                     DRAEAEEVLQESFWRVWNQAATFDPAKGLFRAWLFSIARRQALDLLRRRNVRPQAARD
                     ENEERRFELTPAPDNVGEAAEQAIAAEQMRGALGRLSGEQLQVLELAYFKGLTRQEIA
                     RTTGLPLGTVHTRARLGLQNLRSFMGAANDE"
     gene            13354..13545
                     /locus_tag="PLC06_02060"
     CDS             13354..13545
                     /locus_tag="PLC06_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89259.1"
                     /translation="MSKNDDDGDKKPVQSGQYMGIGIALGAGLGTALGVAFKNIAMGV
                     AIGVAVGVALGAAMSAANK"
     gene            complement(13631..14689)
                     /locus_tag="PLC06_02065"
     CDS             complement(13631..14689)
                     /locus_tag="PLC06_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRO89260.1"
                     /translation="MKRIFTLLPIAVLISLILILPANTQAEDAPKVRAVMFWSAGCGH
                     CHYVITEVLPPLQKEYGDQLEIVMIELNSETAARRFYAAGAAMGMAPDEMGVPMMIIG
                     EHLLFGSQQIPDELPGLIERYLAEGGVDIPALPGMEGLALPAAAAEGSAAEQAATPSI
                     EETTGISGSIPAFVVLVSLSLALIVVGVLLAMARRGSATPPTGGWVSWAIPVLALIGL
                     AVAAYLAYIETQAAEAVCGPVGDCNAVQSSSYARVFGIPVGIIGIAGYLGILGAWVWG
                     RSGNATARLLLLGMAAFGVAASIYLTWLELFVIEAVCMWCLSSAVIMGLILPAAAAWL
                     ATAWTPPKSKLRRRRATG"
     gene            15112..15738
                     /locus_tag="PLC06_02070"
     CDS             15112..15738
                     /locus_tag="PLC06_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRO89261.1"
                     /translation="MRIRIDGMSLAGVALLLLFAVNIVRGWAGEGDPALATTNAEGTP
                     VVVMTAVPAAITPIATAAPPQPAETWNGTIALPYDEYFLTQGVHGASYGHAAIDIAAG
                     NGETIKSIINGNVTGLGYDQWSNTFIQIENDYYVALYMHGVYSPVLGETVTAGQAIGT
                     ESNIGYTLDMAGNSCAGRDCGYHTHLNVYDKRLGMNIDPLTLIPASYP"
     gene            complement(15774..16385)
                     /locus_tag="PLC06_02075"
     CDS             complement(15774..16385)
                     /locus_tag="PLC06_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89262.1"
                     /translation="MSPLSILTHATRRCWDEWISVVLASALWLMAQALIITGPPATAA
                     LFAAARNTDDGVYWGAADLWRDFRALFVPAWKWGLLNLLVIGPALYNLSVFWSVPGVW
                     QWLRPVWLVGLLVWLGLNLFYWPLWLAAEDKSIRNTYANCVRFWLLHPVAALVLFAIC
                     LAAALLLMPFALPIVLGVVFWIALVAETAVRQSLEGMTAGRSE"
     gene            complement(16468..17238)
                     /locus_tag="PLC06_02080"
     CDS             complement(16468..17238)
                     /locus_tag="PLC06_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRO89263.1"
                     /translation="MFIYPFLLAIGTSFKTMPEIAANPSRLIPETFTLEGYQRLFTFD
                     IGRWTFNSAFVAVSITLGNLLFASLAGYALARIPFPGSRLAFLGILGTMMIPGIVLII
                     PMFIVLKSLGMINTYQGLIIPKLTAAFGIFLMKQFFESVPKELEEAARMDGANAFQTF
                     FRVVLPTARPAMVALIIFSFQGAWNDFMHPLIVVTTAKQLWTLPLGLALLRGGMGQNL
                     LWNSLLAGSMLTTLPMAIIFFIFQRYFIEGISYSGIKG"
     assembly_gap    17329..17428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17429..18457)
                     /locus_tag="PLC06_02085"
     CDS             complement(<17429..18457)
                     /locus_tag="PLC06_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRO89264.1"
                     /translation="MAAMSGIKGEDRERAIAGWAFMAPTLIIFAIFIIIPIFFAVYFS
                     LTDWNGISAPRDAAFIGFKNYRALLFQSGIRQTDFFKALKNTTYFAMGVVPAQTILAL
                     LLAVIVNQTWLRFKGFFRTTYYFPSITSSIAISLMFLFFYQRSGLINQVLSAVTFGRW
                     EPVAWMADPRGLFHIMLEAVGITLKTAPDWVKTQVLGLTIWDWISGPSVALMGIMIMN
                     TWTTIGTMMVIFLAALQSVPGYVYEAAQIDGASAWRQFRSITVPLLRPTLFFVITLGL
                     IGTYQVFDQIYVMSSGGPAKTTLTVAYLVYRNGFTNSDMGLAAAIAILLFVIIFTLTM
                     IQRRITGSE"
     gene            complement(18572..19948)
                     /locus_tag="PLC06_02090"
     CDS             complement(18572..19948)
                     /locus_tag="PLC06_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRO89265.1"
                     /translation="MSKKWFALVALLIIAMLAVACGGGGTTQAPAATEAPAVEAPTEE
                     PAVEEPTEAPAAEEPTEAPAAEEPTEAPAETTGQMIEMSLMGWSSSDAENVRLQEMVD
                     TFNAANPDLHVTLNQVPDYDTALQTAMAGGSPPDVFYIDSFKLPDYVKAGALLPIGDQ
                     MDDPDDFYPSLKAAFTIDGTFYCPPKDFSTLGLQYNKDMFDAAGLDYPTADWTWDDLR
                     SAAEKLTDADAGVYGMTLSADFARMIAFVYQAGGTVTNDDFTAMTLDTPEALEAAEFY
                     IGMVTDGYAAAPADLDSGWAGEAFGKGKAAMAIEGNWIASFLNDQFPEINWGVTEMPA
                     GPGGDATMAFTVCYGVAASAPNPEASIRLANWLTGADGMAQWTGLGLAMPTRQSLSDG
                     WLAEYPDLEPFLAGAAYAHPWQFRPGFQDVLDTINSGLQEAFTGASTADLVLSQAQEV
                     GNEVLGGQ"
     gene            complement(20143..22404)
                     /locus_tag="PLC06_02095"
     CDS             complement(20143..22404)
                     /locus_tag="PLC06_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HRO89266.1"
                     /translation="MALDELRHAATPDTGFVVAEDSFDPAAIPGEHRHMETVFTLGNG
                     RFASRGTFEEGFPGESALTFAHGVWDDTPVVFTELVNLPNWLRLSIAIDGDEFRLDQG
                     EILDYRRHTDLRRGILRRAVTWRAPGGPVVDLLFERFHAYDREHVGAIRLLLTAVNEP
                     CAVVVRAGIDGHVANEDLLHLRPAGQGRHDDGTIWLAAETRHTRRRAVVAASASAATS
                     TGTTLPASTENCPGFPALCFAADLTPGQTMTVDKAAIILTDRDIPAGDSLTEAAAAHL
                     RDLSYDRTRGGHVAAWARVWERGDVIIEGDDEAQLAMRFNLFQLLVAAPQTDDRVSIG
                     AKTLSGYGYRGHIFWDTEIFILPYLIYTYPRTARNMLMYRYHCLPGARRKAAGNGFSG
                     AQFPWESAETGDEVTPRWVPHFADPTRLVRIWTGDIQIHITADIAYATLQYWRITGDD
                     EFMRDYGAEMILDGARFWATRAEPETDAGGRVTYSIRDVIGPDEYHEHVDNNSFTNYM
                     GRWQLEMALDIYDWLRGNYPVAADALRDRLGLSHDEFARWRDVIHGIRFLYHPDTDII
                     DQFEGFFDREPVDPELFRSADRSLQVVFGIEGANERQVLKQADVIMLLCLFRDEFDER
                     TWRVNYDTYMPITDLRFGSSLGPSFHAWAACEVGRPDEAYTHFMLAARADLRNPRGNA
                     GDGIHAASTGGVWQAAVFGFAGLRHTADGPTLRPRLPRHWRRLAFTYEYHGRSCHIDI
                     RQDEGGEYRISES"
     gene            complement(22459..23025)
                     /gene="pgmB"
                     /locus_tag="PLC06_02100"
                     /pseudo
     CDS             complement(22459..23025)
                     /gene="pgmB"
                     /locus_tag="PLC06_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            complement(23040..>23235)
                     /locus_tag="PLC06_02105"
     CDS             complement(23040..>23235)
                     /locus_tag="PLC06_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRO89267.1"
                     /translation="AQYLHPPLTSVRQPITEVGRAIVRLLLARLGSEAAAPEGILLRP
                     TLIVRPSSHFTLHPAMEIAQ"
     assembly_gap    23236..23245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23246..24118)
                     /locus_tag="PLC06_02110"
     CDS             complement(<23246..24118)
                     /locus_tag="PLC06_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRO89268.1"
                     /translation="MVDDSKSSRRPSMSDVARLAGVSQTTVSFILNDTPGSNIPLETR
                     ERVLAAVHELGYRPNISARNLRTQSTNLIGFGFDESADVTSRPVIDRFIFSAILSLEA
                     AGYHLLTFVAEKRTDTKAYLELYRRGQVAGFVLANTNDNDPRIARLIEENIPFASFGR
                     SDDTWSFPWVDVDGINGMEQIATHLAACGHRRIGLITWPEGSKAGSHREQGYAAGLAA
                     AGIAPDPAWIVRGENYVQTGAEGMAALLALPPERRPTAVACVSDLIAVGALNAANAAG
                     IAVGRDLAITGYDDS"
BASE COUNT         4274 a         7380 c         8241 g         4218 t
ORIGIN      
        1 gagtccggca atagctgtta acgtgcgctc ttccagacga ccgtgcatga tctttactaa
       61 agcatggcac acgatctata atttctccag tcgctgactg gaggaaccat catgccatca
      121 tcggacgagc agagagcgca attggaggcc ggaattgccg cgctggaagc ccagcgagcc
      181 accctgggcg acgaaatggt cgaggccata cttggcccgg ctcgtgaaaa gctggccgcc
      241 ctcgaatccc gaagccaatc ccaacagcag cgcaagcagg ccacagtgtt gttcgccgac
      301 gtctccggct tcacagccat gtcggagacg atggatgccg aagtcgtggc cggggtcatg
      361 aacgaattgt gggcggaggt cgacagggcc gtcaccgacc atgagggccg catcgacaag
      421 cacatcggcg atgaggtcat ggccctgtgg ggcgcggata cagcccgcga ggacgacccg
      481 gagcgggcgg tgcgggcggc cctggcgatg caggcggccg tcgcccgttt ctgcgagaca
      541 cgcggcatga ccctggccat gcgcgtcggc atcaacaccg ggccggtggt gctcggcgcg
      601 atgggcacga cggccgagtt cacggccatg ggcgacgcgg tcaatgtggc ttcgcgactg
      661 gaacacgccg cgccggtcgg cggcatcctc atcggccacg acacctacag ccaggtgcgc
      721 ggcatcttcg acgtcctgcc gcaggaccct ctcagcatca agggcaaggc cgagccggtg
      781 cggacctatg tggtgcagcg ggccaagccg agggccttcc gcatggccac gcgcggcgtg
      841 gagggtgtcg agacgcgcat gatcggccgc gacgccgaac tggaaacgct gaagaacacc
      901 tacctggacg tgatggagtc ggccgagacg cgcatggcgg tcgttgtcgg cgaggcgggg
      961 gtgggcaaga gccgattgct gtacgagttt gaaaactgga tcgaaatgcg gccggagatg
     1021 atctggtatt tcaagggacg cgctacatcc aacgcgcaga acgtggccta cagcctgttc
     1081 cgcgacctgt tcgccaaccg cttcgagatt ctggagagcg atcccatcgc cactgccctg
     1141 gacaagttcc gccgcggcat ggccggggcg ctggagccgg actcggccga tatagtcggg
     1201 tactggctgg gcttcgactt ttccggcagc gaggccgtcc agggcttgct cggcgactcc
     1261 ggcctgatgc agtcggcgcg ggctttcctg atgcgctact tccgcgccct gaccgcggac
     1321 agcccgacgg tcatcttcct ggaggacgtc cattgggccg acgattcgtc gctggatttg
     1381 gcggtctatc tggcgacggc cttgccggaa gcgcgtctgc ttctcgtcgc cgtcacccga
     1441 tcgaggttgt tcgagcggcg gccgaactgg gacgaggggc ggacggtctt tcgtcgtatc
     1501 tcgctgatgc cgctgcccaa gcgcagcagc cgggcgctgg tggatgagat cctcaagcgc
     1561 gtcgacgaag tgcccgatag cctgcgcgat ctgatcaccg acgccgccga aggcaatcca
     1621 ttctacgtcg aagagatggt caagatgctc attgagcagg gaattatcga gcgcggagga
     1681 gcagtgggga tagggagcgg ggatgactct ctctccccgg ctcctctgct ccccggctcc
     1741 cctgctgcgg aagagaaatg gcgggtgcgc gcggacaagc tggccacgct caaggtgccg
     1801 ccgacgttga cggcgctgct gcaggcgcga ctggacgggc tgccgcggct ggagcgcgag
     1861 gcgttgcagc gagcgtcggt cgtcggcagg ctgttctggg acgacgccgt ggccgacttg
     1921 ctggaggtcg aacgcgagac tgtcacgccg gcgctggagg cggcgcgcgg ccgggaactc
     1981 atctttcgcc gcgagcgctc agccttcgcc gggacgaacg agtacatctt caagcatgtc
     2041 ctgctgcgcg atgtggctta tgagacggtg ctgctcaatc ggcgggccgc ctaccacggc
     2101 cgggccgccc gctggctgga aacccacgcc ggcgagcggc tgagcgaata cctgaccctc
     2161 atcgccgagc actacatcca ggccggggag gggctgcggg cggcggcgct gctggagcgg
     2221 gcgggggacg aggcgatgca ggccggcgcg aacctcgtgg cgcagacgac gctggagcgg
     2281 gcgttggcct tgcgcgaggc ggccggtgag cgtgacggcc cggccgtcga cgccgcgctg
     2341 atcagcatcg gccgcgcaag tcggactttg ggtgactacg cggcggccaa ggcagcgctg
     2401 gagcgcgggc tggtcggggc gcgatccagt ggcaaccgcg cccgggaagc cgaagcgttg
     2461 ggcggactgg cttataacgc cagggtccag ggcgactaca gccatgcgct ggcgctggtc
     2521 gaagaagcgc tacggttggg ccgccctcta ggcgggcggt tactgttaat tttgctggac
     2581 acggcggccc aggtgttgtc tgccatgggc gatctggatg gagccgagag ccgggctatg
     2641 gaggcgctga cggccagccg gactgacggc gatctggccg gcgagcgtat gatccttttt
     2701 acgtttggcg ctgtcgccgc cgagcgccac gagctggaac gatcgaagga atattttctg
     2761 ctgagcctgg aggcggcccg ccggactaac catctgtatg gcgaaggcct ggcgcttctc
     2821 ggcctcggcg agctcgccta ccggctgggc gactacgcca cagcacgaac ttacggccga
     2881 caagccgaag aacgggtgcg agaaatggac atgccggtgc atatcatgaa tgcgttcggc
     2941 aacgtcgccc aggctgagct gaagctgggc gacgtcgccg ccgcccgccg cggcgcctcc
     3001 gaggccctgc agctggcccg ctcgctcggc gccctgcccg atgttctgtg ggcggtcttt
     3061 ctccacggcc agatcatggc cgaggcgggc gagaaggcgc gagggctgct cctcttcggc
     3121 ctggcccgcg cccatccggc gctggattat caggttatgc gggagatcaa cgaggagatc
     3181 gcccggctgg gtctgccgcc ggaggaggtt gaggccgggc tggcggcggg ggcggcgctt
     3241 gacctggaga cggtggtcgg ggagataatg gatgggaagt ggtgagagtg gtatcattca
     3301 tccggatcaa cgatagacag gtcattccat gacgccatac gagctggtct acagcatgat
     3361 gacgcctttt ctgccggttc tttacggcaa ggtcaggcgc gacgtgcgtt cattctcccc
     3421 gcgccggccg ttgactacgg ccaaggcgat ggccggcaat ctcatcaacc gttgggaatc
     3481 gcgcggactc gacgagcaac cctcccgcac cgcccacctt tttgcaatcg cgcgcaagga
     3541 aaaatagttg aacgccaacc ttgttcgacc gtttcggccc accaccgatc tcatccccct
     3601 ggtcgatctg gtcaacgccg ttgagacggc cgaaggacgc gagccatcgc tcaccgtcga
     3661 gcggctggag agcatgctcg acgtccccgg cctctaccgt tgggtggccg tcgcgccgga
     3721 tgccgtcgcg ccggatgatc cgggccgcct cgcgggctat ggcgtcgtct tccaccagat
     3781 gccccaacgc tgctacggcg acgtgcgggt tcatccggcc cggcgtcgcg aaggtatcgg
     3841 ccgccgcctc atcgatccga tggtgcaaaa ggccaccgat ctgggggcgc gctatttgac
     3901 catcgacgtg gatgccgcca atcaggacgg gctgcgcttt ctgctctcgc agggctttcg
     3961 ctttcgcggc gacgtctggg cgctcgttgc cccggctgct gtggcgctgc cgccgccgct
     4021 ctggccggcc ggctacagcg tggcgaccta cgccgattcg cccgatctcc ccggctacgt
     4081 ggagttgtgc aaccgtacct ttggggattt gtgggggcat tgggagaact tccccggcgc
     4141 gatggacgag acccatctga ccggaatact ggagcagttc gatccggccg ggatattcat
     4201 cgtgcgggat tcggccggca aggcggttgc ccaatgccgg gcgaaggcgg ccgcggataa
     4261 cacatcgccc cacatcctcg atcagccggg cgtcattccc gccgccagag atgcgggtct
     4321 gcacgcgccg ctggccctga ctgctgccca ctggctgcgg gcgcaggggg cgcaaccggt
     4381 tcggctggaa tcgtggggcg attcggcggc gacggtcgcg atctacgagg cgctgggctt
     4441 ccatcaaacc ggccacgaac tcagctacgc gcgggagctg ggcgggtgag catcaccatg
     4501 atgcggccac tgatctactg acccactgac cactgatcta ctgacctacc gaccacttcc
     4561 ccatgatcac ccacctcctt cctccccaac tcgacgcggc cacggccacg ctgacggccg
     4621 ccttcgacgc cgacccgatc tacacctacg ccttccccgc ggccaaggat cgcgcgcgct
     4681 atctgccccc gttctggcgg gcgatcttgc gcaatgcgat gctccacggc cacgtctaca
     4741 ccaccgccga ggccgacggt gtggccctgt ggatgccgcc cggccgccat cccgtcgcct
     4801 tcggcgacag cgcccgcatc ggcttcgcct tccatcgggc cgtggcgatg ttcccggccg
     4861 cggcgcgacg gcgattcatg gcctatgtga cctacggcga cggcgtgcag cagcgcttgc
     4921 tgggcgattc gctctattgg gagctgtggg tgctgggcat ccgaccggag cggcaggggc
     4981 aggggttggg cggccaactc ctcgcgccga tcctgaatcg ggcggacgac gagcgcttcc
     5041 cctgttatct gctgaccgag agcgagcgca atgtcgcctt ctatcgcaag catggcttcg
     5101 acgtggccca cgagggtgac gtgccggggc atccattgcg gttgtggatg atgattcggc
     5161 agccgcgata atgtaacacg ggggcagtcg cgcggcagca ggtagttgtg gagcggccgc
     5221 tcgccgacgg cccagacgac cggccggccg gggtcattgg ggtggccggg gcgggccggc
     5281 cgcggcgcga accggatgat gtcgggctgg tcgctgacgt ggtagaggcg ttccgcaagc
     5341 ataaatcacc gggggcatgt ctaacccgca atttgaattc ggcaccggat agggtaccat
     5401 caaatggcaa gttaatatgc tactaaaagg taaatcgtaa agcgaatatg gcaaacaaga
     5461 cattccccgg tcgaaacgaa gatttctcgg aatggtataa ccagctcgtc cttcaatccg
     5521 aaatggccga ctacgccccg gtgcgcggct gcatggtcgt cagaccgtat ggctgggcgt
     5581 tgtgggagaa catttcgtcc tggcttgacg cggagttcaa gaaaacaggt cacgtcaacg
     5641 ccgcgttccc gaccctgatc ccgttgtcgt tcttcgaaaa ggaaaaggaa cacgtcgaag
     5701 gattctcgcc gcagctggcc atcgtcaccc atggcggagg tgaggaactc gaagagaaac
     5761 tggtggttcg cccgacatcc gagacgatgt tcggctacat gtattccaaa tggatcaaat
     5821 cctggcgcga cctgcccgtt ttgatcgttc agtggggttc ggtcctgcgt tgggagctgc
     5881 gcacgaaact gttcctgcgc accgccgagt tctattggca cgaaggccac acggcccacg
     5941 cgaccgccga ggaagcccag gaggagatgc tccgtattct cgacatctac gagaagttct
     6001 cgctcgaaga agcggctatt ccggtcatca aagggcgcaa gaccgactcc gaacgcttcc
     6061 cgggggcgca gatcacctca tcgatcgagg ccatgatgtg ggacaggcgc gccctgcagg
     6121 cgggcacttc ccattacttc ggccccaact tcgccaaggc gttcgacatc cagtatctgg
     6181 acaagagcaa caccctgcaa tattgcgaga cgacctcgtg ggccatctcc agccgcatga
     6241 tcggcgcgat catcatggtt catggcgacg accagggcct tgtcctgccg ccgcgcctcg
     6301 cgccgattca ggtggtgatc gtcccgatct tcaagagcga tgccgaacag gccgcggtga
     6361 tggaagctgc cgatcgcatt ttcgcggagc ttaaggcggc aggcgtccgt gtcaaactgg
     6421 atgaccgcga caatgtgagt cccggattca aatttaacga ttgggagatg cgcggcgtgc
     6481 cggtgcggat cgagattggg ccaagagatg tggaaaacaa gtccgtcgcc ctggcccgac
     6541 gcgaccggtt gggcaaggaa ggcaaatcct ttatcccgca ggcaaacctc gtggtggaag
     6601 tggagcagct tctggtggcg atccaaagca gccttctcca gcgcgctacc gaataccgcg
     6661 atgcccatat ccatgaaccc gccaactacg acgaattcag gcagatcatc gccgatggct
     6721 gggcgtatgc gtggtggtgc ggccgcgccg agtgcgaggc caaaatcaaa gaggagacgc
     6781 gggcgacaac gcgctgcatc ccggccaatc agcccgatga gcatggcgct tgcatctatt
     6841 gcggccgacc ggcgagcgag cgggtctact tcgcgcgcgc ctattgagag gcgcgtgagg
     6901 aaggaacacg cacgggagaa agggagcagg gggctccctt tctccccttc tcctactaaa
     6961 tcacccccgc ctctcccagc gccttgagcg tgatcagcaa ccccgcgacg gtgatgaatg
     7021 tcgcgctggc tatcggcagc aggcgggtgg tcatagccgt gtggcggatg ggcaggcgct
     7081 ccagcagctg cccggcatag accatgaaca ggccgatgcc ggtcagcacc cccgccagcc
     7141 cgacgctgaa ggcgacgatc agcacgatcc ccagccccac ctgatgcagg gcgatggcgc
     7201 tgagcataag gatgagcgcc gaggggcagg gcaagatgcc gccggagatg cccaacgcca
     7261 gcaggttgcg ccggctaagc ggctcggccg cgtggtcggt gggcagaggt gattggtcgg
     7321 cttgatgctc atgatcgtgg gtatggctat ggtcatgggc atggtcatgg tcatggtcat
     7381 gggcgaccag gggggaaggg gcgacggccg ccggctgcat ggctccggca aaagccaacg
     7441 agcgcgccgg ggccatcgtg acggccgcat tggctgaact tgtcgcctgc tgccggccgt
     7501 gggcatgggc atggccgtgg tcgtgatggt cgtggctgtg tgggccatga tggtggtcgt
     7561 ccggcccatg agagtggccc ttgccgaaat ggaagtgata ttgctcatcc gccccggcca
     7621 ggccgcggcg cagcgccagc tggttgcgca gatgtccgcg caaaatcgac gcgccgatga
     7681 aaaccaccag cagccccgac aacacgccca gccagggata gagcttctcc ggcaggatga
     7741 attcggaagc ggccagcacc agaaagccca gggcaaaaac cccggccgtg tgggtgatgg
     7801 tggtgatgag gcccaggaac acggcgtggc gagccttgcc gcgcgaaccg acgagatagg
     7861 ccccgacgat ggttttgccg tggccgggcg tcagcgcgtg ggccgcgccc aacgcaacgg
     7921 ccagcagcag caccgccggc agcgcccccg gcgtgtcgag cgtgcggttg agcaggttgg
     7981 cgaactcgtc ctggccgaag cgattggcgg ctgcggcggc ttgctgcccg cccattgtcg
     8041 tcggctgccg gtcgccggtc gttgcgccgt ccatgggtgc atagcgcatc tcggcccgat
     8101 tgacggccga tggggccgtc agcaggtcgg ccggatagtt gcgcaattgg tcgctgaggt
     8161 cggcagcggg cacggacgaa tcgagcagcg agccgtcagc cgcggtggcg atcacttcct
     8221 gccagcccgg tcgcccggcg aaattgccgt cctcataggc cagcgcgcgc ggcgcgtcgc
     8281 cgtcgacggc gggcagggcg gccgacaggc tgatctccag tcgcagcgtc tccagattgc
     8341 cctggccggg cgggaagctg agggcgtggg atgtgggcga caaggcaacg gccgcgccgt
     8401 cgaccgtcag gcgcagattg gcggccagat cggcgatggt ttgctcgacc caggcgtctt
     8461 cttcggccgc cgaggccgtc ccgtcgccgt cggcgtccat ggcctgccgc tcctggaagg
     8521 tggggatttc ggccatgtcg atgatataga gcatatcgac ggcggccgcg cgcagcgtca
     8581 gggcgctata gcggctgatg gtgaagttgc ccagcgggtg ggcagcgacg atgccggcca
     8641 cgctgagggc cagcagggcg agcagcacgg gaagcgcccg gcgaatgatg cgtttgcgaa
     8701 tgatggattg ccctttcatc atgatctcca gtttcctgac agatttgtag ttagccgtta
     8761 cccctcaagc ccgtccagcg tctcgcgggc ggtttgggcc tgcaacaagt cgaaccacgg
     8821 gttcagatcg agcgcgcggc cgagatactc cctggcggcg gcgacgtcac cgcgcgcggc
     8881 ggcgatcatc cctgcgtggt agaacagcag cgcgtcctgc gtgccgaggg ccagcgcttt
     8941 atcagaggcg acgcgggctt cgtcgtagcg gccggcgcga tagagcgccc acgccagcgt
     9001 gtcataggcg tagatgtcct gccgggcggc cagttcggcc tcggcatagg ccagcgcggt
     9061 gtcggcttgc agcgcatgat tggcgaagaa gacggccatc tgtcggttat agagaacctg
     9121 ttgcgccaca tccagctgct ggatgaacag caccgtatca tattgcgcct gggcggcagc
     9181 cgtgtcgcct tgcagcgcgt agagatcgcc gaggaaggcg acgaagttgg gctgcggcag
     9241 ccgcgccacc agcgttgtgt agcgggcaat ggcttcatcg agatggcccc gcgccgcctc
     9301 gatcttgccc agcccgctct gggccagata gtgaccgggg aatgcctggt cggcctcgtg
     9361 ataccacttt tcggcattgc gcaggtcgcc ggtgttgaaa tatagctcgc ccaactggaa
     9421 gcgataccag gccaattcct cgccgccgat gtcgagggcg agtgtttcat cggccgcctt
     9481 tttcatccag tcaatcgcct catccggccg cccctggagc cagaccagtc gcgccatccg
     9541 gctgtagatc gcgccgccgg gatatttctg cagcaagtgg cgataggctt cctcggccgc
     9601 cgggtagctg cccagctcaa ggttggcgtc gccggcgacg gccagtgcct gatattcgtc
     9661 gggattggtg gccagcgccg cctgggcgaa ggtcagcgcg tcggcgaagg cgtgttggcc
     9721 gatcaatacg ctgccgatga gcgccttggc cggttcatac ttggggttga attccagcgc
     9781 cttgcgaaag gattcctcgg cgcgttgata cagggcggcg tcccccgtct cgcgcgcttc
     9841 ctgcaggtag gcctggccca ggtgggtcat ggtgatgtaa tcctgggtgt tcggccccag
     9901 ccgattggtc cagaaatcaa cctgctgcgc cggggacatg atgtcgggga ttggcggctc
     9961 gcccggcccc aggaccatct ccagcagcgc gggcacgccg gtgaagttgc tgccggcgcg
    10021 cagcgcgtag gacgcaatca ggatgagggc ggtgaagaga accagcaggg tcagcttgcg
    10081 attttcggat acggtgctca tgatcgattc gccttgcctc gtggggcgct tggcggccgg
    10141 ttgcccgtcg tgtcggcggc gggcaaccgg ctcgcggtgg atccgctcag gcagatgcct
    10201 gttgggccat gcctagctgt taggctcggc caggtagggg aagttggtca tgaacggctg
    10261 gtcgttcatg ttcacgcagt cgccggggat gccgggcgcg ccgatgaggc cgccggtgac
    10321 gacggtcagc tcgaagtcga tcacatcctc ggccaactgg cggccgttca gggtgcccac
    10381 gaagccgttg gtcgagttcg tgtccagggt gagcacgtcg ggcagcaaca ggccggtgat
    10441 gacgccggcc gtggcgtcgt catagccgcc gaacagcccc agcaccgcct tgaagcggtc
    10501 gccccacatc gccggatcgt tggccggggt ggtggcgttg taggcgtcct tgtcggccgc
    10561 atcgatgaag acggtgttga gcgccggctt gcccatgcgg tcgacgacag tcatgcgatt
    10621 cacggtgcgc gcccagacgt tgatcatgcc gtcgtcgcta tcgagaaggt cggatggtat
    10681 ctcgaccacg atggcggtgc cgttcagccc ggcgaagaag tcaacctcat tgccgtcgca
    10741 gaaggtacgg ctgccgcccg cgccgccgat ctggtcgcgg aacgcttgca ggtcgaagaa
    10801 gaacgggtca tcgcgcaggc cgacatagaa ccagccaccg gtgcgcagcg gcagattttt
    10861 atcgctcaga ccggcgccga tgcgacgctg ggttccagca tccacatctt gccaatagag
    10921 ctggacgcgc tggcgtcccg acttttgcgg ccggtcggct cgcagcttca acactgcgtc
    10981 ttccatgtaa tcgccgtcgt tgtcgatggc gaactcatag cgggtgccgg gggtgctgcg
    11041 cagggtcgtg gggctgagga cgcccgcggc ggggttgacg gtcatgacga ggacggtgtt
    11101 gtcgccgtgg acaaaggcgt agacgtcatt gatgtcgagc cgcccgtcct gcttgacgat
    11161 gggcgcgtcc agatggtcgg ccgctccggc gcccagcgcc gccaggccga ggaccatggc
    11221 cagcatcaca acggccatga cgataaaggt acgaactttg ttcatctcgg aatctccttg
    11281 tctgcttggg tgtgttcaat gaaacggatg aatcgaaggt ggatgtcggt ttgcctgatt
    11341 ctgattgttg cctgacctcc tccggccgat gggccgaatc gtatgcctgt ggattatcaa
    11401 tattgttcct ggcggcgcgc cgttgggcgt tggagcattg gggcgctgcc tgctaacaga
    11461 cagtacgcag cgtgttgctc cctgcattta tggagttgga gatcaaaaat cggcggcaaa
    11521 ttcaagttgg ggtgcggaca ggagcgcggg cgtggcaaca aaaaaggcgc gcctcgaaag
    11581 ggcgcgcccg gcgacaaacg acgagggttg gtttggcggc tagagcatgc ggccgagcag
    11641 gataattttg ccctgcgtgg gggtggcgct gccgccttcc ggctccatgg aaacgccgat
    11701 ggcgtcgaac tcgggtggca gctcgccgct caggatgtgg gtgccgttgc cgttggcatc
    11761 cacgacgaac atcccggcgc tgattggcgt ctcgccgacg atgacccacg cctggtaggt
    11821 gtcgccggac gagggcaggg gcagattgct gacgcgcaat gtggtctcgc cggtagccat
    11881 gtcggtggtc agcgtgccca cggccgcggg gtggtcaccg gtggcgtcgc cgatggcgac
    11941 cgttctcatg ccgggatggc ggaactgggc cagcaggtct tcacgttccg ccagcccgcg
    12001 ccgcagcacc tcgttttcgt tcgtcagcgc gtcgagttgg gcgcgcagac cggcgtcgtc
    12061 ggcttgcagc gcggcgatct gctcttgttg ggcggcattt tggcgggaaa ggcgggcgac
    12121 ggccgcggcc gagacgatga gggctaccag ggcgaaggcg gtcagcgcca tggccggtcg
    12181 gccggaagtg aaccagtctc gccaggaacg gcgcgcggcg gccggccgag gttcggcggc
    12241 ggggcggggc ggtggcgcgg cggccgcgac ctggggagtg gctggacggg catcggccgc
    12301 cacgcgcgcc atcagcccgg ctttcacggc gggggcgggc gcgatcggcg gggtcgtcgc
    12361 cattgtcagg tcggccgcgt cccgcatcag cacggccaga cgcgccgccg ccgccggatt
    12421 gccggccaca tagccgtcta cctcggcccg ttcctcttcg gtcagggcgt cgagcgcgta
    12481 gaacggaaaa agctcttctt cgatacgctg attgcgttcc atgatctact cgtcattcgc
    12541 cgcgcccatg aacgagcgca ggttctgcag ccccagccgg gcgcgcgtat gcacggtgcc
    12601 cagcggcagg ccggttgtgc gggcgatctc ctgtcgggtc aaccccttga aataggccag
    12661 ttcaagcacc tggagctgct cgccggacag gcggcccagc gctccgcgca tctgttcggc
    12721 ggcaatggcc tgctcggccg cttcgcccac gttgtcgggt gcgggcgtca actcgaagcg
    12781 gcgttcctca ttctcatcgc gggccgcctg cggccgcacg ttgcgccgcc gcagcaggtc
    12841 gagcgcctgt cgccgggcga tgctgaacag ccaggcgcga aacaggccct tggcggggtc
    12901 gaaggtcgcg gcctggttcc agacgcgcca aaacgattcc tgcagcacct cttctgcttc
    12961 cgcccgatct tgcacgattc tgacgataat gcccagcgtg atcggcgcgt agcgatcgta
    13021 gaggcgctcg aaggcatccg ggtcacgtgc cgtgactcgg gccatgagtt cctcatcgct
    13081 tgtgccggtc atctgatcca taccgtgctc aatggcgcga tgcggtgcga caggatgtgt
    13141 gatgcgttcc tcataacccg ggcctgcggc ctggggggaa cgcgcgtgtt gaatttgcac
    13201 ttgtcctgta tacgcagcgc cgcgctattt gaatcttaac cgatttgacg ggatttcgat
    13261 agagggcaga tccgccccca ttgccgaaca gcaaccatgc atgataccat ccgacaaata
    13321 cgggccgggt ctcaatcggc cgaaggagtt accatgtcaa agaatgatga tgacggagac
    13381 aagaagccgg tacagagcgg gcagtatatg gggataggaa tcgccctggg ggcaggtctg
    13441 ggcaccgcgc tcggcgttgc cttcaaaaac atcgccatgg gcgtagccat cggcgtcgcc
    13501 gttggcgtgg cgcttggcgc ggcaatgagc gccgccaata aatgaccggc gcggggagac
    13561 aggctgtctg tcgcccctaa atcggcctct ctgcggtcgc gtaaagggcg aatcccaact
    13621 ccatattagc ctagcctgtc gcgcggcggc ggcgcaattt cgactttggt ggcgtccatg
    13681 ccgttgccag ccaggccgcg gccgccggca ggatcaaccc catgatgacc gccgagctga
    13741 gacaccacat gcagaccgcc tcgatgacga acagctccag ccaggtcaga tagatggagg
    13801 cggcgacgcc gaaggcggcc atccccagca gaagcagccg cgccgtggcg ttacccgatc
    13861 ggccccagac ccacgcgccg agtatgccca gatagcccgc gatgccgatg atgccgaccg
    13921 ggatgccgaa cacccgcgcg tagctgctcg attgcaccgc gttacagtcc cccaccggcc
    13981 cacagaccgc ctcggccgcc tgcgtctcga tgtaggccag ataggcggcc accgccaggc
    14041 caattagcgc cagcaccggg atcgcccacg agacccagcc gcccgtgggc ggggtggcgc
    14101 tgccccggcg ggccatcgcc aatagcaccc caacgacgat cagggccagg cttaggctga
    14161 cgagaacgac gaaagcggga atgctgccgg agatgccggt ggtctcttcg attgacggag
    14221 tggcggcttg ctctgcggcg ctgccttcgg ccgcggcggc cggcagggcc aacccttcca
    14281 tgccgggcag ggccggaatg tcgacgccgc cctcggccag atagcgctcg atgaggccgg
    14341 gcagctcgtc ggggatttgc tgggagccaa acagcagatg ctcgccaatg atcatcatgg
    14401 ggacgcccat ctcgtcgggg gccatcccca tcgcggctcc ggcggcatag aagcgccggg
    14461 cggccgtttc ggagttaagc tcgatcatca cgatttcaag ctgatcgccg tactccttct
    14521 ggagtggggg gagcacctcg gtgatcacat agtggcaatg accacagccg gcgctccaga
    14581 acatcaccgc ccgcaccttg ggggcgtctt cggcttgcgt gttggccggg aggatcagga
    14641 tcaggctgat gagaacagcg attggcaaga gggtgaagat gcgtttcatc ggtttggctc
    14701 cacgccggag atcgagctga cgcccgaatc cgggatagca tggtttgcga tcgacagcgc
    14761 cgaaacgcga cagaggggca accgcccacg gcctgccgtc ggcgtctatg aggagtatag
    14821 ggcatggcca cccgaacaat tagtgatcta cgtcacggtt tgggcgaata aatgtcactt
    14881 gtttttgggg atcggcgtaa cttcgggttg gacgaaaaaa ggacagaaaa aggacatatt
    14941 acttgttgca aaatagtaca aacgttctat aatttagaag gtttcggcca atcagaggcc
    15001 tgacgggaac gggaacccgg ctgatggcgc aaagcgccgg cgaggcactc tgaaaatctg
    15061 tgaaatttgt gaaacctgtg gatccttctt ttcttatatt gatggattga aatgcgcata
    15121 cgaattgatg gaatgagcct ggccggggtg gccctgttgt tgctgttcgc ggtcaacatt
    15181 gtccgtggct gggcggggga gggcgacccg gcgttggcca cgacgaacgc tgagggcacg
    15241 cctgtcgtcg tgatgacggc cgttccggcg gcgatcacac ccattgcgac ggccgcgccg
    15301 ccgcagccgg ccgagacctg gaacgggacg atcgccctgc cgtatgatga atatttcctg
    15361 actcagggcg tccacggcgc gtcctatggt catgcggcca tcgacatcgc cgccggcaat
    15421 ggcgagacga ttaaatcgat catcaacggc aatgtcaccg ggctgggcta cgatcagtgg
    15481 agcaacacct tcattcagat cgagaatgat tactatgtgg cgctctacat gcatggggtc
    15541 tattcgccgg tgctggggga gacggtcacg gcggggcagg ctatcggcac ggagagcaac
    15601 attggctaca ccctcgacat ggccggcaac tcatgcgccg ggcgcgattg cggctatcac
    15661 acccacctca acgtctacga caagcgactg gggatgaaca tcgacccgct gacgctcatc
    15721 ccggccagtt atccataagg acggggagtc gtgcgcccct ttgccggccc gttttattcg
    15781 ctgcggccgg cagtcatccc ttccaggctt tgccgcacgg ctgtttccgc caccagggcg
    15841 atccagaaca ccacccccaa cacaatcggc agggcgaagg gcatgagcag cagcgccgcg
    15901 gccagacata tggcgaacag gacgagcgcc gcgaccggat gcagcagcca aaagcggacg
    15961 caatttgcat aggtattgcg tatcgacttg tcttcggccg ccagccacag aggccagtag
    16021 aacagattca gccccagcca gaccagcagc cccaccagcc acaccggccg cagccattgc
    16081 cacacgccgg gcacgctcca gaacaccgac aggttataga gcgccgggcc gatgacgagg
    16141 agattgagca gcccccactt ccacgccggg acgaacagcg cccggaagtc gcgccacagg
    16201 tcggccgcgc cccagtagac gccgtcgtcg gtgttgcggg cggcggcgaa cagcgccgcc
    16261 gtggccggcg gcccggtgat gatcagcgct tgcgccatca gccacagtgc gctagccagc
    16321 accaccgaga tccattcgtc ccagcagcgc cgcgtggcgt gggtgaggat agagaggggg
    16381 gacataacaa aatacgaggt acgagatacg agatacgagt tcagaaggcg cgctcttcac
    16441 tcgtatctcg tacctcgtat ttcgtatcta tcctttgatt ccgctatagc tgattccctc
    16501 gatgaagtag cgctggaaga tgaagaagat gatcgccatc ggcagcgtcg tcagcatgga
    16561 gccggccagc agcgagttcc acagcaggtt ctggcccatg ccgccgcgca gcagggccag
    16621 gcccagcggc agcgtccaca gctgcttggc cgtggtgacg acgatcaacg ggtgcatgaa
    16681 gtcgttccac gccccctgga agctgaagat gatcaacgcc accatcgccg ggcgcgccgt
    16741 gggcagcacc acgcggaaaa aggtctggaa ggcgttcgct ccgtccatcc gcgccgcttc
    16801 ttccagctct ttcggcaccg attcgaagaa ctgtttcatc agaaagatgc cgaacgcggc
    16861 cgtcagcttg ggaatgatta gaccctgata ggtgttgatc atgccaagcg acttcagcac
    16921 gatgaacatg gggatgatca gcacgatgcc ggggatcatc atggtgccca aaatgcccag
    16981 aaaggcgagg cgactgccgg ggaacgggat gcgcgccaac gcataaccgg ccaggctggc
    17041 gaagagcaga ttgcccagag tgatcgacac ggccacgaag gcgctgttga acgtccagcg
    17101 gccgatgtcg aaggtgaaca gccgctgata gccctccagc gtgaacgtct cggggatgag
    17161 ccgcgacggg ttggcggcga tctccggcat cgtcttgaag ctggtgccga tggccagcag
    17221 aaacggatag ataaacatga tcgccagcag gatcagcacg ccataggaca ggaaggcctt
    17281 gagcggcttg gcccacgggc gctcgccgtg ggtcgtggtg gtggatgann nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct cgctgccggt gatgcgccgc tggatcatgg
    17461 tcagggtgaa gatgatgacg aacagcagga tggcgatggc cgcggccagc cccatgtcgc
    17521 tgttggtgaa gccgttgcga tagaccagat aggctacggt caaggtcgtc ttggccgggc
    17581 cgccgctgct catcacgtag atttggtcaa atacctgata ggtgccgatg aggccgaggg
    17641 tgatgacgaa aaacagcgtc ggccgcagca ggggcacggt gatgctgcga aattgtcgcc
    17701 acgccgacgc gccgtcgatc tgtgccgcct catagacata gccgggcacg ctctgcagcg
    17761 cggccaggaa gatgaccatc atcgtgccga tggtcgtcca cgtgttcatg atcatgatgc
    17821 ccatcagcgc cacgctcggc ccgctgatcc agtcccagat cgtcaggccc agcacctgcg
    17881 tcttcaccca gtcgggggcg gtcttgaggg tgatgccgac cgcttcgagc atgatgtgga
    17941 acagtccgcg cgggtcggcc atccaggcca ccggttccca gcggccgaag gtgaccgccg
    18001 acagcacctg attgatgaga ccgctgcgct gatagaagaa caggaacatc aggctgatgg
    18061 cgatcgacga ggtgatggac gggaagtaat aggtcgtccg aaagaagccc ttgaagcgca
    18121 accaggtctg gttgacgatg acggccagca gcagcgcgag aatcgtctgc gccggcacca
    18181 cgcccatggc gaaataggtg gtgttcttga gggccttgaa gaaatcggtc tgccgaatac
    18241 cgctttggaa caacagcgcg cggtagtttt tgaaaccgat aaacgccgcg tctcgcgggg
    18301 cgctgatgcc gttccagtcg gtcagactga aatagacggc aaaaaagatg gggatgatga
    18361 tgaagatggc gaagatgatg agggttgggg ccatgaaggc ccaaccggcg atggctcgtt
    18421 cgcggtcttc acctttgatg cccgacatcg cggccatgat attttcctcc catgtggcgc
    18481 aatctttaga ttgcgtcttc cctgtagcgc aacccgccgg attgcgggga cgcaacctgg
    18541 acaggttgcg ctacaggtta cgactactta tttactgacc gcccaaaacc tcattgccca
    18601 cttcctgtgc ctgactgagg accagatcgg cggtggatgc gccggtgaat gcttcctgca
    18661 gcccggagtt gatggtgtcc agcacgtcct ggaagccggg ccggaattgc cacggatggg
    18721 cataggcggc cccggccagg aacggctcca ggtcgggata ttcggccagc cagccgtcgc
    18781 tcagactctg gcgggtgggc atggccaggc cgaggccggt ccattgcgcc atgccgtcgg
    18841 ccccggtcag ccagttggcc agccggatgg aggcttcggg attgggcgcg cttgcggcca
    18901 cgccgtagca cacggtgaaa gccatcgtcg cgtcgccgcc cggcccggcc ggcatctcgg
    18961 tgacgcccca gttgatttcg gggaactgat cgttcaggaa cgaggcgatc cagttccctt
    19021 cgatggccat ggcggccttg cccttgccga acgcttcgcc tgcccagccg ctgtcgaggt
    19081 cggccggcgc ggcggcgtag ccgtcggtga ccatgccgat gtagaactcg gctgcctcca
    19141 gcgcttccgg cgtgtcgagg gtcatggccg tgaagtcatc attggtcacg gtgccgcccg
    19201 cctgatagac gaaggcgatc atgcgcgcga agtcggcgct gagggtcatg ccatagacgc
    19261 ccgcgtcggc gtcggtcagc ttttcggccg cgctgcgcag gtcgtcccac gtccagtcgg
    19321 ccgtcggata gtcgaggccg gcggcgtcga acatgtcctt gttatattgc agccccaggg
    19381 tgctgaagtc cttgggtggg caatagaacg tgccgtcgat ggtgaaggct gccttcaggc
    19441 tgggatagaa atcgtcggga tcgtccatct gatcgccgat gggcagcagc gccccggcct
    19501 tcacatagtc gggcagcttg aagctgtcga tgtagaagac gtcggggggc gagccaccgg
    19561 ccatagccgt ctggagcgcg gtgtcgtagt ccggcacctg attgagcgtg acgtgaaggt
    19621 cgggattggc ggcgttgaag gtgtcgacca tctcctgcag gcggacgttc tccgcgtcgg
    19681 agctggacca gcccatcaac gacatctcga tcatctgccc ggtcgtctcg gccggggcct
    19741 cggtaggctc ttcggccgcc ggggcttcgg tgggttcttc ggccgccggt gcttctgtcg
    19801 gttcctcaac tgccggttct tcggtaggtg cttcgaccgc cggggcctca gtggccgccg
    19861 gcgcttgagt tgttccgccg ccgccgcaag ccaccgccaa catggcgatg atcagcaggg
    19921 caacaagcgc aaaccatttt ttggacatgt ctcttctcct tgaaatttat gatggtcctg
    19981 taatgcgcca atgacggcgg ggagatcgtt aatgtgttaa aatctcgtcc gctgggcgca
    20041 cgcctcgcta atgccgcgta accattgctt tgaccggccg gttacgcggt atctttttct
    20101 ttagaagccg agtttcccgg aagaggctcg gcttccggtg ggttagcttt cagatattcg
    20161 atactcacct ccttcatcct gtcggatgtc gatatggcag gatcggccgt gatattcata
    20221 ggtaaatgcc agccgtcgcc aatggcgggg caggcggggc cgaagagtgg ggccatcggc
    20281 ggtgtggcgc agcccggcaa agccgaagac ggccgcttgc cacacgccgc cggttgacgc
    20341 ggcgtggatg ccgtcgcccg cgttgccgcg cgggttgcgc aggtcggcgc gggcggccag
    20401 catgaaatgg gtatacgcct cgtcggggcg gccaacctcg cacgcggccc aggcgtggaa
    20461 cgacgggccg agcgaggagc cgaagcgcaa gtcggtgatg ggcatgtagg tgtcatagtt
    20521 gacccgccat gtccgctcat cgaactcgtc gcggaacaaa cagagcagca tgatgacgtc
    20581 ggcctgtttc agcacctggc gctcgttggc gccctcgatg ccgaacacga cctgcagcga
    20641 acggtcggcg ctgcggaaca gctccgggtc gaccggctcg cggtcgaaga acccctcgaa
    20701 ctggtcgatg atgtcggtgt cggggtggta caggaagcgg atgccgtgga tgacgtctcg
    20761 ccagcgggcg aattcatcgt ggctcaagcc cagccggtcg cgcagcgcgt cggccgcgac
    20821 cgggtagttg ccgcgcagcc agtcgtagat atccagcgcc atctccagct gccaacgccc
    20881 catgtagttg gtgaagctgt tgttatcgac gtgttcgtga tattcgtcgg ggccgatgac
    20941 gtcgcggatg gaataggtga cgcggccgcc ggcgtccgtc tccggctcgg cgcgggtggc
    21001 ccagaagcgc gcgccgtcga ggatcatctc cgcgccgtaa tcgcgcataa attcgtcgtc
    21061 gccggtgatg cgccaatatt gcagagtggc ataggcgatg tcggcggtga tgtgaatctg
    21121 gatgtcgccg gtccagatgc gcacgaggcg cgtcgggtcg gcgaaatggg gcacccagcg
    21181 cggcgtcact tcgtcgcccg tctcggcgct ctcccacggg aactgcgccc cggagaagcc
    21241 gttgccggcc gccttgcgcc gtgctcccgg caggcagtgg tagcggtaca tgagcatgtt
    21301 gcgcgccgtg cgggggtagg tgtaaatcag gtagggcagg atgaaaatct cggtgtccca
    21361 gaagatatgg ccgcgatagc cgtagccgct gagcgtcttg gccccaatgc tcacccggtc
    21421 gtcggtttgc ggcgcggcca ccagcagctg gaacaggttg aagcgcatcg ccagctgggc
    21481 ctcgtcgtcc ccttcgatga tcacgtcgcc ccgttcccag acgcgcgccc aggcggccac
    21541 gtggccgcct cgcgtccggt catagctcag gtcgcgcagg tgggcggcgg ccgcctcggt
    21601 cagactgtcg ccggcgggaa tgtcgcggtc ggtcaggatg atcgccgcct tgtcgaccgt
    21661 catcgtctgg ccgggggtca ggtcggcggc gaaacagagc gcggggaagc cgggacagtt
    21721 ctcagtcgag gccggcaaag tcgtgccggt cgacgtcgcc gccgaagccg acgcggccac
    21781 gacagcccgc cgccgggtgt ggcgcgtctc tgccgccagc cagatcgtcc cgtcgtcgtg
    21841 ccgcccctga cccgccggcc gcaggtggag caaatcctcg ttggccacgt gaccgtcgat
    21901 gccggcgcgg acgaccacgg cgcacggctc gttgacggcc gtcagcagca gccggatggc
    21961 gcccacatgt tcgcggtcgt aggcgtggaa ccgctcgaac agcagatcga cgaccgggcc
    22021 gcccggcgcg cgccaggtga cggcccggcg caggatgccg cgtcgcaaat cggtgtggcg
    22081 acgatagtcg agaatctcgc cctggtcgag gcggaactcg tcgccgtcga tggcgatgct
    22141 caggcgcagc cagttgggca ggttgaccag ctcggtgaag acgacgggcg tgtcgtccca
    22201 gacgccgtgg gcgaaggtca gcgccgattc gccggggaac ccttcctcga aggtgccgcg
    22261 tgaggcgaag cggccgttgc ccagcgtgaa gacggtctcc atgtggcgat gttcgccggg
    22321 gatggccgcc gggtcgaagc tgtcttcggc tacgacgaag ccggtgtcgg gggtggcggc
    22381 gtggcgcagc tcgtcgagag ccacgccggt caggtcgtcg cgccgggcga aatggccgca
    22441 gccggcgagg gcggcgaatc gctcggccgg gccaagggcc agcgccgcca tgcccgccgc
    22501 caacgccgct tcgatgcccg cggcggcgtc ctcgaccacc aggcagcgaa cagcgggcac
    22561 gcccagcccg gcggcggcga agcggaacag gtcgggcgcc ggtttcggcc gctccacgct
    22621 gtggccgtcg gccaatacgc ccagccgttc ggcgatgccc agccgcttgc agaccgccgg
    22681 cgcgttcttg ctggccgagg cgatgccgta ggggatggcc gccgcgtcga gttggtcgag
    22741 caggtcgacc acgccgggca gcagatcggc cgggctgatg cggtcgagca gggcctcata
    22801 ataggtctgt ttgcgggcca tcagcgcctg catgcgctcc tcgctctccg ggcggccgcc
    22861 cagcacgacc agcagcgact cgcggcgact gacgccgcgg caatgctcgt tggccacgcg
    22921 gtcccacgcc agcccttcct cgtcggccag ccgctgccag gcctgatagt gatactcggc
    22981 cgtgtcggtg atcacgccgt cgaggtcgaa gatgacggcc cggagcgggg cgacggaatt
    23041 cattgcgcga tctccatggc ggggtgcagg gtgaaatggc tggatggacg aacgatcagc
    23101 gtcggccgca gcaatatccc ttccggggcg gccgcttcgg agccgagccg cgccagcagc
    23161 aagcgaacga tggcccggcc cacctcggtg atgggctggc gaacggaagt gagcggcggg
    23221 tggaggtatt gggccnnnnn nnnnngctgt cgtcgtagcc ggtgatggcc agatcgcggc
    23281 cgacggcgat ccccgccgcg ttggcggcat tgagcgcgcc gacggcgatc aggtcgctga
    23341 cgcaggccac ggccgtgggg cggcgttcgg gtggcagcgc caggagggcg gccatgcctt
    23401 ccgcgccggt ttgcacgtaa ttctcgccgc gcacgatcca ggccgggtca ggcgcgatgc
    23461 ccgccgcggc cagtccggcg gcgtagccct gctcgcggtg ggagccggcc ttcgagcctt
    23521 cgggccaggt gatgaggccg atgcgccggt ggccgcaggc cgccagatgc gtcgcgatct
    23581 gctccatgcc gttgatgccg tcgacatcaa cccagggaaa agaccaggtg tcatcggagc
    23641 ggccgaagct ggcgaagggg atgttctcct cgatcagacg ggcgatgcgc gggtcgttgt
    23701 cgttggtgtt ggccagcacg aacccggcca cctggccgcg ccgatacagc tccagatagg
    23761 ccttggtgtc ggtgcgcttc tcggcgacga atgtcagcag gtggtagccg gccgcttcca
    23821 gactgaggat ggccgaaaag atgaagcggt cgatgacagg gcgggaagtg acgtcggccg
    23881 attcgtcgaa gccgaagccg atgaggttag tcgattgggt gcgcaggttg cgggcgctga
    23941 tgttggggcg gtaacccagc tcgtggacgg cggccaggac gcgttcgcgg gtttccaggg
    24001 ggatattgct gccgggcgtg tcgttgagaa tgaacgacac ggtcgtctgc gagaccccgg
    24061 cgagacgggc gacgtcgctc attgacggac ggcgggatga tttggaatca tcgaccatgg
    24121 agcaacttcc tcttaaaaac tgcgaacggc agtactaaat ggaattagta atacgtatta
    24181 gtataaaatt aaacaggaag gattgtcaat aggtagaagt aac
//
LOCUS       DAOMQG010000016        22597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_982_length_22597_cov_10.437539, whole genome shotgun sequence.
ACCESSION   DAOMQG010000016 DAOMQG010000000
VERSION     DAOMQG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 22597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22597
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_22597_cov_10.437539"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="PLC06_02115"
     CDS             <1..1653
                     /locus_tag="PLC06_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89269.1"
                     /translation="PHSSLVPAVAGLLAALSPVLVYYSQETRMYALLALLAALSAWVL
                     LAWLGGVRRPLPWMAAYTLLLVAGLYTHYFFPAVVVAQGAVVVWREWGKGRRGEREKG
                     GRGERERGSGGALLNSYFVSRISYLGQWVGMAAVAALLYLPWVPVFLRQIGGRGEAAG
                     LGEFAMESARWLALGTTVAPDEATWAVVAYVALVALGVAAGRRRAVVALLLALIPLAL
                     MFLVGATDPAFFKFMLVVVPFLAVLAGAAWDDQLREGAAVDGRIRDIAIGLVAAVLTV
                     AVLAGSLLSLGNLYFDPAYARADYRAMAARIAAEAHPNAGIILVAPNQWEAFTYYHRD
                     GAPVYPLPRGRPDPAVIEPELARIAATHDRLYVLYWGDAQRDPEHIIERWLDAHAFKA
                     SEEWVGDVRFVVYAVPGDAPAEATPSGATFAGLNGESITLHEYAVWPGEARPGDVIQA
                     RLVWSADATPARAYKVFLHLLDEAGNVVAQHDGEPAGGSRPTTTWAAGEQVIDNHGVL
                     LPSGLPSGDYTLRLGLYDALDPGARLAVDGADGLELGRIVIP"
     gene            1735..8880
                     /locus_tag="PLC06_02120"
     CDS             1735..8880
                     /locus_tag="PLC06_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRO89270.1"
                     /translation="MVGLSGIRVTVAGGRQWILLAPMALLLIMLPLPSVVALPADDSP
                     PTVAAVSPLPDATDVALAAATDGLLTASFSEPVVLATGAIELACERSQVHAVTTSGGP
                     VTFAFMSNRSFLPGERCVVTIHAEQVSDLDEDDPPDLMGIRYEWSFMTTGAPVMINEL
                     DAVSADDLGEFVELYDGGQGNADLSGLTVVFYRGDEASVYLAVSLNNLETDADGYFVL
                     GDSGVPGADLPLANGTLLDGPDAIAVYAAPRSAFPNGTPVSLVNLLDAVVYGPADERL
                     LVLLNAGQDPLDEDARGAATTDSNQRCPNGGGQPQETETFIQNNPSPGEGNHCRFDDP
                     PQVDDVVPKPSAVDVALDTSLAVTFSEPVSLTGQPFEIVCTATGIHAYDVAGGPETFA
                     ITLHEPLTYGETCVNTVWADEVRDLDSDDPPDTLAAKYTWTFQTVKWVAENVVINEID
                     ADTLGVDAAEFIELFDGGVGGTSLDGLNIVFFNGADDLSYLTINLNGRQTNQAGYFVL
                     GNESVNGVGITFPNGALQNGPDAVALVTGAATDWPNGTAITGVIPLDALVYGRPTQVA
                     GGLMPLLNAGQPQVDEAGRGDVEAHSNQRCPNGAGGARNTTGYKQNTPTPGATNDCAT
                     DTAPNVIDFSPARDAQGVASTTAIVVEFDEPVSVTGKWMTVKCVTSGTHTYTTTGGPI
                     EFVVTPTEPFAYDETCTVTVNPDLVTDQDTDDPPDKLAKKVSWSFVTGGEPADFVLIN
                     EIDADTPGTDTAEFIELYDGGRGLTALDGLLIVLYNGSNNLLYQTIDLGGFSTDEQGY
                     FVLGDTAVTPDLVLANGFLQNGPDAVALFIGGADQFPNNSPITLQGLLDAGVYGASEA
                     VSQDLLRLLEAGQEAADENGRGAADTHSLQRCPDGSGGQRRMDSYAPNTPTPGVPNRC
                     EIDLFPTIISTIPAVGATGVSVYASINLTFSEAVDVASGSIRLECGTQGDLSVAISGG
                     PLSYTVSPAAPMPQEATCRLTVVATGVSDQDDDDPPDKMAEDYQLEFTTGHVGADFVI
                     INEMDADTPGSDKAEFIELFDGGRGNTSLSGLLIVLYNGGDDRSYRMVNLTGLKTDSD
                     GFAVIGNKDVAGVGMELPVGFLQNGADAVALYAGGPDSFPNGTAVHTQGLIDALVYGT
                     DDPADKGLLILLEAGELQVDEAGTGAAEQHSVGRCPNGEGGPRRTATYRTSLPTPGIA
                     NDCEIVVEDDPPAIVEISPADGESHVSLDSAIRLRFSEAVELGPGWFTIDCQSSGLHA
                     ATVAGDATEVVLQPQEPFSSGESCTVKIEAELVSDIDTNDPPDYMFQDFQSTFQAVVV
                     AQGYALINEIDPDTPGNDSAEFIELYDGGDGGTPLSGLVMVWWNGEDDTIYRVIDLNG
                     QATNSEGFLVIGNAAVNPAVIFGNGALQNGPDAVALYAGDAADFAIGMPMTTVGLVDA
                     VVYGSGVADEGLLALLEAGESQLDENDRGDREAHSLARCPDGAGGPRRTAGYAAGEPT
                     PGHHNACMPPDAPPAVASVSPQPDSVNVPLGASLIITFTEEMEADAGWLGLSCAGSEL
                     ALDVTGGPQVFVAIPRSPLPSGAQCTATIHAVAVHDADTDDPPDILPEDYSWQFTALA
                     DTPLPPVAGFVSNGPVWVGETVVFTNKSSGPGPLTYSWDFGDGSPVSDELHPTHYYRR
                     MGTWTVRLTVTGPTGTDIAGETVDVRPRRLFLSVITRQALVGIAGKPIRPTIIPMSLP
                     KHAMPIDAGFDVEFADALARYETYNKHHYRPNTYLHKWWGRRCGSTFRLILKGLVEDE
                     AARPYYAPGGLAGKVILDPMMGGGTTLHEAIRLGASVIGVDVDPIPVAQARAALTHRP
                     AGSLRAAFDTLYDELAAAAGDLFQTHCPTCDERVPLWYALYGWRRVCGCGEVLAVDSL
                     TLRHEPDGTTLRWCPACGQLRNGDGHACDSGGVRLVEKGVTRCADCGESYRDPVDTPY
                     YARYAMLAVAGHCPAHGLFHKSPDWRDQEALARADERRASLPLPREQFRVVPGDKSIQ
                     LINRGIDSYLDLFSSRQLIVLSEVIRQLPDEDAIIRLNLALLVSTSLEFNSMLCGYKG
                     VNKRRAGAVRHTFAHHGYAFPHTALENNPLYPRRASGTLQKLFESRIARGRRWAARPL
                     ERSLSEQPSRFVAIEGEMDCGQEVAAASELAGGSHRFMLIRGSSVALPVESGSIDAVV
                     TDPPYFDSIQYGDLSAFFRVWLRQFLPGAADWSYDQSTAAVNSERDNENGQYVDLMTG
                     IFRECRRVLRPGSGRLIFTFHHWQPRAWAALTLSLYGAGYSLVNRYVVHAENPISVHI
                     SNMRALTHDAILVLAASGHEGPAWSERPPLPDHLDSYRFTESCASFLGWMLAQPGLTP
                     CLIETEWQAYLASDTSA"
     gene            complement(8882..9706)
                     /locus_tag="PLC06_02125"
     CDS             complement(8882..9706)
                     /locus_tag="PLC06_02125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89271.1"
                     /translation="MSTKPKFRRTTRYIRTHWPAFVGLYLALVAGVLLIGLSLAFDWY
                     SFIPFSLAIMLVVAYFLVAYVYVAYRLNDPPGGTATEFLIDLAQLQAHHRVVCIDLGL
                     KATAVTIAQHLTTGQVTVIDIYNPQSNIGGALRRARGRAHRPPPDPRLNWIDGNIQLL
                     PLPDRSTGAVFMNQILSEFWIPEEREQLLAEVRRILIPEGRLLVAERVRSLANPALTG
                     IVTYPLPTEKEWLGALTKAGFILRRQENAGGVVYCVRVDRPSPTSGKQMLLDLEYD"
     gene            9850..10461
                     /locus_tag="PLC06_02130"
     CDS             9850..10461
                     /locus_tag="PLC06_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO89272.1"
                     /translation="MIDKPDPYSRFLDRYEAGPVPWDDPQPPPEIIALAAELEPGRAL
                     DLGCGYGRVSIYLAQRGWSADGVDFIPRAIEVAQERAAAAGVADSARFHVASAAELGF
                     LTPPYDLAVDIGCMHSFNELMLRGYRSGLMRLLRPGGLYVLFAHLRDEDEPVIDDGPR
                     GIPERVIRELLVDGFELERVEHGVTQVEGRPPWNSGWFWYRRR"
     gene            complement(10481..11602)
                     /locus_tag="PLC06_02135"
     CDS             complement(10481..11602)
                     /locus_tag="PLC06_02135"
                     /inference="COORDINATES: protein motif:HMM:NF023462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3524 domain-containing protein"
                     /protein_id="HRO89273.1"
                     /translation="MRILLLSPYHGGSHRAWAEGYRSASRHEVDLLTLPAHFWKWRMH
                     GGAVTLARHWLAQNSPAPDLILSTDLHDLTTFLALTRRQTAAIPVALYMHENQLTYPL
                     PTDPTIGPMRRQLGERDRHYAFVNFISMLAADRVFFNSRYHLDTFFTALLPFLRHYPE
                     YNEMESIASLRQKSDVLHVGVDLRRLDLQSGDAPLSDPPLVLWNQRLEYDKNPEGFIN
                     VLVALAAEGIPFRVALCGERFGKPGERWESGVAELGERVIHNGYADDDLYRHLLWEAA
                     VTVSTAGHEYFGISILEAIYCHTFPLLPARLSYPELLPEAFHADCLYHGRPDLMRRLR
                     WALNDSAAARDVARRLAPHTARYDWGTMAPLYDDVLGRI"
     gene            11722..11937
                     /gene="iscX"
                     /locus_tag="PLC06_02140"
     CDS             11722..11937
                     /gene="iscX"
                     /locus_tag="PLC06_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRO89274.1"
                     /translation="MSENEPELYWDSTYAIVVSLMEHYPERGPIDVGLAELVEMVESL
                     PGYQDDTALVNEQLLLDILTVWYEEAT"
     gene            11940..12635
                     /gene="nuoB"
                     /locus_tag="PLC06_02145"
     CDS             11940..12635
                     /gene="nuoB"
                     /locus_tag="PLC06_02145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRO89275.1"
                     /translation="MTVANTGTAGGDVAVPDELANNVFITNIAKLLDPVYNWSRRNSI
                     WPVTFGLACCAIEMICTASSRYDLARFGMEVFRATPRQADLMIISGTVTKKMVPTIVR
                     LYNQMPEPRYLLSMGACASGGGPFKEGYNVVDGIDKFLPVDVYVPGCPPTPQALINGL
                     IALQEKIDQQSIATSPWYNKKDQTAGLYPVPVLGPDLVDLRQLNIIRQEAAKYEAEQA
                     AAEVPSAEAEPAA"
     gene            12671..13150
                     /locus_tag="PLC06_02150"
                     /pseudo
     CDS             12671..13150
                     /locus_tag="PLC06_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            13361..14494
                     /locus_tag="PLC06_02155"
     CDS             13361..14494
                     /locus_tag="PLC06_02155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257853.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRO89276.1"
                     /translation="MKTDRVTVNLGPQHPSTHGVFRMVVTLDGETIVDLKPVMGYLHR
                     NHEQIGERNTFNMNMPYTDRLDYLSSMSNNLGYAITIEKLFDITVPERAEWIRILMVE
                     LTRICNHLWALGFLLNDLGALQTPMLYFYLERELILDFFESAAGARLLCNYMRFGGLA
                     YDLPDDVRGVETMKFLEELIYNRLPKVLDQGDDLMTGNEIVRARAIGVGILTPEDAIA
                     MSAAGPLLRASGVPYDVRRAEPYSYYEHLDFDVAVRYNGDIYDRYLIRMDEMRQSLRI
                     LEQVLPHLRQTAGAPIINGKPEYAFRAPRAGASYGRVENPKGELGFYVTAKRRAGNPD
                     RYHVRAPSFINLTALGPMARGHKVADVVAILGSIDIVLGEVDR"
     gene            14593..15216
                     /locus_tag="PLC06_02160"
     CDS             14593..15216
                     /locus_tag="PLC06_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRO89277.1"
                     /translation="MDDLKWAGKGGRYYNEEAFAVRQGPMGEGVKTVFYPEEKLSTPE
                     RFRFVPFLVTDDPPPGQRWGHDWCTSCGICAKACPPQCIWIERGKQPNGRPKPEPEKF
                     FIDIDICMNCGYCAEFCPFDAIKMDHDYELASYDRRTAHIHDKERLSKPLSYWREIAP
                     KKAEAEELARQMAEAAKTKKKDKDGETRDARLAEAVSRQLYYQGARY"
     gene            15397..16473
                     /locus_tag="PLC06_02165"
     CDS             15397..16473
                     /locus_tag="PLC06_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRO89278.1"
                     /translation="MFGNKKDNPGVTQETVMKALSTVIEPDLHRDLVSLNMIRNLQID
                     GNDVTFTIMLTTPACPLRGKMEGDSRAALARVPGIGQVTINWDSNVPADRRIGEQVGQ
                     NFRNTIAVSSGKGGVGKTTVAVNLAISLAMEGARVGLLDADILGPNVPMMMGREQMPT
                     PVNRKMIPAENYGVKFISMAFLVKPDQPLIWRGPMLHSAIRQLLTDVNWGELDYLIVD
                     LPPGTGDAQLTLAQVLPLSGALVITQPMQVAASDAVRGLKTFEKLEVPIIGVIENMSG
                     DFFGTGAGEQLAKEYQTTYLGSIPLEAEVRKGGDSGRPIVVAHPDSEAAKAIRRIARD
                     VAARVSVLTLQVQADNIIPISMIG"
     gene            16610..17554
                     /gene="ricT"
                     /locus_tag="PLC06_02170"
     CDS             16610..17554
                     /gene="ricT"
                     /locus_tag="PLC06_02170"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRO89279.1"
                     /translation="MTTVMAPGETSDFIGVRFQKLGKLYHFKVGSHKDVVVGDHVIVE
                     TKRGKQLGQVIAFIDPEEIHRQKGLRAVQRKATPRDLVMKQVWESKELDALIACREEA
                     SKADIKDAKFVKAEYSFDGSWLTIAYTTENKKLDVRQVQSALGRIYRTRVEMQLVGPR
                     DVAKMMGGYGACGIPRCCSTFLTDFSPISIRMAKEQGISLSPQEITGMCGRLRCCLVY
                     EYEQYVEAKKHLPKVGKTIGTPYGEGRVYDVRPLRDSVMVDVGEESHEVFRHEIEPIE
                     ELEALKKKAEAGCSKHENGGCDCGAKRPKSQDDPGEGE"
     gene            17556..18155
                     /locus_tag="PLC06_02175"
     CDS             17556..18155
                     /locus_tag="PLC06_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRO89280.1"
                     /translation="MKTRADAWDTVCRYVQERGLRRHMLAVSIAMADYARRLNEDAAY
                     WETVGLLHDFDWEIHPNLNEHPGKGADILRAEGWDEETIRVILSHYTEGTGVEREKPI
                     DFALLACDEITGLIIATTLVKASRNIADVTTDTLRKKWKDRRFAAGVDRDAVAAETAD
                     FSRVSFDGTLELWDHIGNVLGAMQAAAAELELDGRLAVQ"
     gene            18177..19439
                     /locus_tag="PLC06_02180"
     CDS             18177..19439
                     /locus_tag="PLC06_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRO89281.1"
                     /translation="MAKQEDLSVREALNLILGTIDILSSEELNLFDGLGRVLAQSVTA
                     HDSLPPFANSSMDGYAVRAEDVGGAREDRPIRLKVIGDIAAGAGILPEVIAGTAARIM
                     TGAPLPPGADAVVPVEDTDEPWRGKERPLPEFVAIHRAVGPGDFTRHPGEDIRAGDIV
                     LEKGCVLRPQEIGILAALGVGRINVIRRPRVGVLATGDELVDVASPLTPGKIRNSNGY
                     AQTAQVAALGGVPVMLGVAGDTEAAVRERLDAGVAAGVDLLISSAGVSVGAYDVVKAV
                     LDEVGDVAFWRVRMRPGKPLAFGSYRGTPFLGVPGNPVSAMVSFEMFARPAILKMAGH
                     DKLERPRVEVTILEDIDSDGRESYIRAVVTRDERGYLARTTGGQGSHMMTSLVKANAL
                     LVVPEGVKAIQAGTKLKALMIDWSGGIF"
     gene            complement(19476..20321)
                     /locus_tag="PLC06_02185"
     CDS             complement(19476..20321)
                     /locus_tag="PLC06_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRO89282.1"
                     /translation="MSAETIEMTPEIITEAAPTSIADLSPKMQLKGTVKRLELYGAFI
                     DLGIDATGLIHISKLGGDQVNRVSDVLNEGDEVTVWVEKVDPERQQVMLTMVPPLAVD
                     WTELKTDQVYEGKVTRLETFGAFVSIGAEREGLVHISELSHNYVKSPSEVVNVGDEVQ
                     VKVLGFNRRKRRIDLSMKALVEKPEMAPMQPSQPRQNSGGGKRRQSRERFEPEVYEII
                     EDETEEVPTAMEIAMRRALGNDAISAVKTEQRGGRKGKKAVREDQRRVQDDLLKRTLT
                     MQSDN"
     gene            complement(20555..21625)
                     /gene="recA"
                     /locus_tag="PLC06_02190"
     CDS             complement(20555..21625)
                     /gene="recA"
                     /locus_tag="PLC06_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRO89283.1"
                     /translation="MSRSNGSNTQDGRQATLDKTIDSLTKRFGEGTIMRLGEGHNLHI
                     QVVPTGSISLDIALGVGGVPRGRIIEIYGPESSGKTTLCQHIVAEAQKLGGICAFIDM
                     EHALDPSYAAKCGVDVDNLYVSQPDTGEQALEIADALIRSGTMDVVVIDSVAALVPRA
                     EIEGEMGDTHVGLQARLMSQALRKLSGAIKQTNTVVIFTNQLREKIGVMFGNPETTPG
                     GNALKFYASVRLDIRRIQAIKAGNDVIGNRTRVKVKKNKVAPPFTECEFDIMYNEGIS
                     KTGDVLDLATNYNIVDKRGAYFRYNDMLLGQGRENAKVFLAENPTVLLELERRIRNES
                     HITAIAVEAVAADGGDSEEVEF"
     gene            complement(21774..>22597)
                     /locus_tag="PLC06_02195"
     CDS             complement(21774..>22597)
                     /locus_tag="PLC06_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRO89284.1"
                     /translation="VMLGVIQIADLIIIRLASGLPPGSTSSYFYGYGLMQFPQTLFGT
                     AIALVVFPTLAELYNARDIEGLKRTASSTLAIIWTLTIPAAAATVLLGRPIIETLFQG
                     GAFDEQATRMVYAVLAILSIRIVSESTLEIVARLFYARHNTFTPMLAYIGWFVVTGAI
                     SFLLVRQMGVVGLALASAVGFTLLAIVLFELNRRDLNGLGGRPLAQSFLRATIGAAAM
                     SLAIWLIGRVVSGTLPFLFAGLIIGGSVYLVVTWLLGGRELRSLLQIARSKTIST"
BASE COUNT         4384 a         6304 c         7257 g         4652 t
ORIGIN      
        1 cctcattcgt cactcgtccc ggctgtggct ggtctgctcg ccgctctcag cccggtgctg
       61 gtctactaca gtcaggagac gcggatgtac gcgttgttgg cgctgctggc ggcgctgagc
      121 gcgtgggtgc tgctggcgtg gctgggcggg gtacggcggc cgctgccctg gatggccgcc
      181 tatacgctgc tgctggttgc cgggctgtat acccattatt tcttcccggc ggtggtggta
      241 gcccaggggg cggttgtcgt gtggcgggaa tgggggaagg ggagaagggg agaaagggag
      301 aaagggggaa ggggagaaag ggagaggggg agcgggggcg cgcttctgaa ctcgtatttc
      361 gtctctcgta tttcgtatct cggccagtgg gtggggatgg cggccgttgc tgcgctgctc
      421 tatttgccct gggttcccgt ctttttgcgg cagatcggcg ggcggggcga ggcggcgggg
      481 ttgggcgagt tcgctatgga gagcgctcgc tggctggcgc tgggcacgac cgtcgcgccg
      541 gacgaggcga cgtgggcggt cgtcgcctat gtggcgctcg tcgctctcgg tgtggcggcc
      601 ggccgccggc gggcggtggt tgccttgctg ttggctctca ttcctctcgc cctcatgttc
      661 ctcgttggcg cgaccgatcc ggcgttcttc aagttcatgc tggttgttgt gccgtttctg
      721 gccgtccttg caggcgctgc ctgggatgac caattgagag aaggggcggc cgtcgatggt
      781 cggatacggg atatcgctat cgggttggtc gctgcggtcc ttaccgtcgc cgtcctcgcc
      841 gggagcctgc tgtcattggg gaacctctac ttcgatcccg cctatgcgcg ggccgactat
      901 cgagccatgg ccgcccgcat cgccgccgag gcccatccca acgcgggcat catccttgtc
      961 gccccgaacc agtgggaggc gttcacctat taccaccggg acggcgcgcc ggtctacccg
     1021 ctgccgcgcg gccgacccga cccggccgtc atcgagccgg agctggcgcg catcgccgcc
     1081 acccatgatc ggctttacgt gctctattgg ggcgatgccc agcgcgaccc cgaacatatc
     1141 atcgagcgct ggctcgacgc gcatgcgttc aaggcgtcgg aggagtgggt gggcgatgtg
     1201 cgcttcgtcg tctacgcggt gccgggcgac gccccagccg aggcgacgcc cagtggggcg
     1261 accttcgccg gactgaatgg cgaatcgatt actctgcatg agtacgccgt ctggcccgga
     1321 gaggcccggc cgggggatgt gattcaggcc cgcctggtgt ggtcggccga tgcgaccccc
     1381 gcgcgcgctt acaaggtgtt cctgcatctg ctcgacgagg cgggcaatgt ggtggcccag
     1441 cacgacgggg aaccggccgg cggctcgcgg ccgacgacga cctgggccgc gggggagcaa
     1501 gtgatcgaca atcatggggt gctgctgccg tcgggcctgc cgtcgggaga ctatacgctg
     1561 cggttggggt tatatgacgc gctcgatccc ggcgcgcggc tggcggtcga tggggcggac
     1621 gggctggagt tgggccggat cgtgatccct tgattttgtt tgctctcacc acaggctcgt
     1681 tataattgcc tcaacgggat aacgtggcgg gttcttcctc aggaggttgg cccaatggtg
     1741 gggttatcag gtattcgcgt gaccgtggcg ggcggccgcc agtggatact cctcgcgccg
     1801 atggcgctcc tccttatcat gttgcctctt ccttctgtgg tggcgcttcc ggcggatgat
     1861 tctccgccga ctgtggcggc cgtgtctccc ttgccggatg cgacagacgt ggcgctggca
     1921 gcggcgactg atggcttgct gacggcatcg ttctcggagc ctgtggtgtt ggcgactgga
     1981 gcgatcgagt tggcctgcga gcgtagccag gttcatgcgg tgacgacgtc gggtgggccg
     2041 gtaacgtttg cctttatgtc gaatcggtcg ttcctgccgg gcgaaagatg cgttgtgacg
     2101 atccacgccg agcaggtgtc ggacctggat gaggatgacc caccggacct gatgggcatc
     2161 cggtatgaat ggagcttcat gacgacgggc gcgccggtga tgatcaacga actggatgcc
     2221 gtctcggccg atgatcttgg agagttcgtg gagctgtatg atggtggtca agggaatgca
     2281 gacttaagcg ggctgacggt ggtgttttat cggggggatg aggcatcggt ctatttggcg
     2341 gtaagcctga acaatctgga gaccgacgcg gatggctatt tcgtcctggg ggacagcggc
     2401 gtgccggggg cggatttacc actggcgaac gggacgctgc tcgacgggcc ggatgcaata
     2461 gcggtttatg ccgcgccgag gagcgcgttt cctaacggca cgcccgtttc attagtgaat
     2521 ctgcttgacg cggtcgtcta tgggccggcg gatgaacggc tgctggtgct gttaaatgcc
     2581 ggacaagacc cgctggatga agacgctcga ggagcggcaa caaccgattc gaatcagcgc
     2641 tgccccaacg ggggcggcca accgcaagag accgaaacct ttatccagaa caacccctcg
     2701 ccgggagaag gcaatcactg ccggtttgat gatcccccgc aagtagatga tgtcgtgccg
     2761 aagccgagtg cggtggatgt ggcactcgat acatctctgg cggttacatt cagcgagccg
     2821 gtaagcctga ccgggcaacc attcgagatc gtctgtacgg cgaccggcat tcatgcgtat
     2881 gatgttgccg gtgggccgga aacgttcgcc atcaccctgc acgagccgct gacatacggc
     2941 gagacctgcg tcaataccgt ttgggccgat gaggtgcgcg atctggattc tgatgatccg
     3001 ccggatacgc ttgccgccaa atacacatgg acgttccaga cggtcaagtg ggtcgcggag
     3061 aatgtggtga tcaacgagat tgacgccgac acgctgggag ttgatgcggc cgagttcatc
     3121 gaattattcg atgggggcgt gggcggtaca tcgctcgatg ggcttaatat cgtgtttttt
     3181 aacggcgctg atgatctcag ctatctgacg atcaacctca acggccgaca aaccaatcag
     3241 gcgggctatt tcgtgctggg caacgaaagc gtgaatgggg ttgggatcac gttcccgaac
     3301 ggggcattac agaatgggcc tgatgcagtg gcgctggtga cgggagctgc aaccgattgg
     3361 cctaacggca cagcgatcac cggcgtaatt ccccttgacg cgctggttta tgggcggccg
     3421 acccaggttg ccggcggttt gatgccattg ttgaacgccg gacaaccaca agtagatgag
     3481 gcagggcgag gagatgttga agcccattcg aaccagcgct gccccaacgg ggcgggtggg
     3541 gcgcgcaaca caaccggata caagcaaaac acaccaacgc cgggagcaac gaatgattgt
     3601 gccacagata cagcgcccaa tgtgatcgat ttctcccccg cgcgcgacgc acaaggggta
     3661 gcctcgacga cggccattgt tgtggaattt gatgagccgg tgagcgtgac cggcaagtgg
     3721 atgaccgtga aatgcgtcac ttcgggaacc catacctaca ctacaaccgg cgggccgatt
     3781 gaattcgtgg tgacgccaac cgagccgttt gcctatgatg aaacctgtac ggtgacggtg
     3841 aacccggacc ttgtgaccga tcaggacact gatgatcccc cggacaagtt ggcgaagaag
     3901 gtctcctggt cattcgtaac gggcggcgag ccggctgact ttgtgctgat caacgagatc
     3961 gacgcggata cgccggggac tgataccgcc gaattcatcg agttgtatga tggcggccgc
     4021 gggctgactg ccctggatgg cctgctcatc gttctctaca acggcagcaa caatttattg
     4081 tatcagacaa tcgacctcgg cgggttcagc accgatgaac agggctattt cgtgctggga
     4141 gacacagccg tcacgcccga tctggtgctg gctaacggct ttctgcaaaa cgggccggac
     4201 gcggtcgcgc tgttcattgg aggcgctgac cagttcccca ataactcgcc gattacactg
     4261 caagggctgt tggatgccgg cgtctacgga gcttccgaag cggtctcgca ggacttgttg
     4321 cgattactgg aagcaggaca ggaagcggcc gacgagaacg gccgtggagc ggccgatacc
     4381 cactccttgc agcgctgccc ggacggcagc ggtgggcaac gaaggatgga tagctacgcg
     4441 cccaataccc caacgcccgg cgttccgaat cgatgtgaga tcgatctgtt cccaacgatc
     4501 atcagcacga tccccgccgt gggagcgacc ggtgtttcgg tttatgcgtc catcaatctg
     4561 acgtttagcg aagctgtgga tgtggcttcg ggctcgatcc ggcttgagtg cgggacacag
     4621 ggtgacctct cggttgcgat aagtggtggg ccactttcgt acacggtcag tccggccgcg
     4681 ccaatgcctc aggaagcgac ctgccggctg acggtcgtcg ccacgggagt gagcgatcag
     4741 gacgacgatg atccaccgga caagatggca gaggattatc aactggaatt tacaaccggg
     4801 catgtgggtg ccgattttgt gatcatcaac gagatggacg cggacacgcc cggtagcgat
     4861 aaggctgagt tcatcgagct tttcgacggc gggcgaggca acaccagttt gtcgggcttg
     4921 ctgatcgtcc tgtataacgg cggtgatgat cggtcataca ggatggtgaa tctgaccggt
     4981 ctcaagaccg atagcgacgg gtttgcggtg attggcaaca aggacgtggc gggagttggg
     5041 atggagttgc cggtggggtt tctgcagaac ggcgccgatg cagttgcttt gtatgcaggc
     5101 gggccggatt catttccgaa tggaacggcc gtgcataccc agggattgat tgatgcgctt
     5161 gtttatggga cggacgatcc ggctgacaaa ggcttactca tcctgttgga agctggggag
     5221 ctacaagttg atgaagccgg gaccggcgcg gccgaacagc attccgttgg tcgctgcccg
     5281 aacggcgaag gcggtccacg gcgcacagcc acgtatcgga cgtcgctgcc cacgcccggc
     5341 atcgccaatg attgtgagat tgtggtggaa gacgacccgc cggcgatagt ggaaatcagc
     5401 cctgccgatg gggagagcca tgtatcgctg gatagcgcca tccggttgcg ctttagtgaa
     5461 gcggttgaac ttggtcccgg ttggttcacc attgattgcc agtcgagcgg tctgcatgcg
     5521 gcgactgtag cgggcgatgc gactgaagta gtgctccaac ctcaggagcc gttttcatcg
     5581 ggcgaatctt gcaccgtgaa gatcgaggcc gagctggtca gcgatattga cacgaacgat
     5641 ccccctgact acatgttcca ggactttcaa agcacttttc aggcggtcgt ggtcgcgcaa
     5701 gggtatgcgc tcatcaacga gatcgacccc gacacacccg gcaatgattc ggccgagttc
     5761 atcgagcttt atgacggtgg cgatggaggt acacccttga gcggcctggt gatggtatgg
     5821 tggaatggcg aagacgatac gatttatcgt gttatcgatt tgaacggcca ggccacaaat
     5881 agcgagggat tccttgtgat cgggaatgcg gccgtcaacc ctgccgtgat ctttgggaat
     5941 ggcgcgctgc agaatgggcc tgatgctgtc gcactctatg ccggggacgc ggccgatttc
     6001 gcaatcggca tgcccatgac gacagtcggg ctggtggatg ccgtcgtata cggctccggt
     6061 gtcgcagatg aaggattact ggcattgctg gaagcggggg agtcgcaact ggacgagaac
     6121 gatcgtggcg atcgggaggc acattcgttg gcgcgatgcc ccgatggcgc aggcggccca
     6181 cgccgtacag ccggctatgc ggcgggcgaa ccgacgcccg gccatcataa cgcctgcatg
     6241 ccgcctgatg cgcccccggc cgttgcatct gtttcgccgc agcccgacag cgtgaatgtc
     6301 ccactaggcg cgtcgctgat catcaccttc accgaggaga tggaggcgga tgccggctgg
     6361 cttgggcttt cgtgcgccgg cagcgaactt gccctggacg tgaccggcgg gccgcaagtg
     6421 ttcgtggcga ttcctcgttc gccgctgccg tccggggcgc aatgcacggc cacgattcac
     6481 gcggtggccg tccatgacgc cgacaccgac gacccaccag acatattgcc ggaagattat
     6541 agctggcaat ttactgccct tgccgacacc ccgttacccc cagtggccgg atttgtatcc
     6601 aatggcccgg tgtgggtggg ggaaacggtt gtcttcacga acaagagcag cgggccaggc
     6661 ccattgacct attcgtggga ttttggtgac ggcagccctg tctcagacga actccatccg
     6721 acccattatt accgccgaat gggcacctgg acggtgaggt tgaccgtcac cgggccgacc
     6781 gggacggata tcgccggcga aacggtcgac gttcggccgc ggcggttatt tttaagcgtg
     6841 atcacacggc aggcccttgt gggcattgcc ggtaagccaa ttcgccctac aattatccca
     6901 atgagccttc ccaaacacgc catgccgatc gatgccggat tcgacgttga attcgccgat
     6961 gcgctggctc gctatgagac ctataacaag caccactatc ggccgaatac ctacctgcac
     7021 aaatggtggg gacgccgttg cggcagcacc ttccgcctca tcctgaaggg gctggtggaa
     7081 gatgaagccg cgcgccccta ttacgcgccc ggcgggttgg cgggcaaggt catcctcgac
     7141 ccgatgatgg gtggcggcac gacgctccat gaggccatcc gcctgggcgc gtcggtcatc
     7201 ggcgtggacg tcgatcccat cccagtggcg caagcacggg cggcgctgac ccatcggccg
     7261 gcggggtcac tgcgggcggc attcgacacg ctctacgatg agctggctgc tgctgccggg
     7321 gacctgttcc agacccattg cccaacgtgc gatgagcggg tgcctctatg gtacgcgctc
     7381 tatgggtggc ggcgggtctg tggctgcggc gaggtgttgg ccgtcgattc attgacgctg
     7441 cgtcacgaac cggacggcac gacgctgcgc tggtgtcccg cctgcggtca attgcggaac
     7501 ggtgacggcc acgcctgcga ttcgggcggc gttcgcctgg ttgagaaagg ggtcactcgc
     7561 tgcgccgatt gcggcgagtc gtaccgcgac ccggtcgata caccctacta tgcccgctat
     7621 gccatgctgg ctgttgccgg ccattgccct gcccatggcc tgttccataa gtcgcccgac
     7681 tggcgcgacc aggaggcatt ggcgcgggcc gacgagcgcc gggcatcgtt gcccctgcca
     7741 cgcgaacaat tccgcgttgt ccccggcgat aaatcgatcc agctaatcaa tcgcggcatt
     7801 gacagctacc tcgatctgtt ttccagccgt cagctaatcg tgctgtcgga agtcatccgg
     7861 caattgcccg acgaggacgc gatcattcgc ctgaacctgg cgctgcttgt gtccacgtcg
     7921 ctggagttca actcaatgct gtgcggctac aagggggtga acaagcggcg ggcgggggcc
     7981 gtgcgtcata cctttgccca ccacggctac gcatttcccc atacggcgct ggagaacaat
     8041 ccgctctatc cgcgccgggc ctcgggcaca ttgcagaaac tcttcgagag ccgcatcgcg
     8101 cgcggccgtc gctgggcggc ccggccgctg gagcgttcgc tgagcgagca gccgtcgcgc
     8161 ttcgtggcga ttgaggggga gatggattgc gggcaggaag tggcggccgc atcggaactg
     8221 gcgggcggtt cccatcgctt tatgctgatt cgagggtcat cggtggcttt gcccgtcgaa
     8281 tcgggcagca tcgacgccgt cgtcaccgat ccgccctatt tcgacagcat ccagtatggc
     8341 gacctgtcgg ctttctttcg cgtgtggctg cggcaattcc tgccgggggc ggccgattgg
     8401 agctatgatc aatcgacggc ggcagtgaat tccgagcgcg acaacgagaa cggtcagtat
     8461 gtcgatctga tgacggggat attccgggag tgccgccggg tgctgcggcc ggggtcggga
     8521 cgactgatct tcaccttcca tcattggcag ccgcgggcgt gggcggcgct gaccctgtct
     8581 ctctacggcg cgggctactc gctggtgaat cggtatgtcg tccatgccga aaaccccatc
     8641 tccgtccaca tcagcaatat gcgcgccctg acccacgacg ctattctggt attggccgcg
     8701 agcgggcatg aggggccggc gtggtctgaa aggccgccct tgcccgatca tctggacagt
     8761 tatcgtttca cggaatcgtg cgcctcattc ctggggtgga tgttggccca gccagggttg
     8821 accccttgcc tgattgaaac ggagtggcag gcgtacctgg cgtcggatac gtccgcttga
     8881 gctagtcgta ttccaaatcc agcagcatct gtttgccgct cgtcggagat ggcctgtcaa
     8941 cgcggacgca atagacgact ccccctgcgt tttcctggcg ccgcaggatg aacccggctt
     9001 tggtcagcgc gcccaaccat tctttttccg tcggcagcgg gtaggtgacg atgccggtca
     9061 gggcggggtt ggccagcgag cgcacccgct cggccacgag cagccgccct tcagggatga
     9121 ggatgcgtcg cacttcggcc agtagttgct cccgctcttc ggggatccaa aattcagaga
     9181 ggatctgatt catgaagacc gcgcccgtac tccggtcggg caggggcagc agttgaatat
     9241 tgccgtcgat ccagttgagc cggggatcgg gtggtgggcg gtgtgcccgg ccacgagcgc
     9301 gccgcaatgc gccgccgata ttggattggg ggttgtagat gtcgatgacg gttacctggc
     9361 cggtggtcag atgttgggcg atggtgacgg ctgtagcctt gaggcccaaa tcgatgcaga
     9421 cgacacgatg atgggcctgg agttgtgcca ggtcgatgag gaactcggtc gccgtgccgc
     9481 ccggcggatc attaagccga taagccacat agacataggc caccaggaag taggcgacaa
     9541 cgagcatgat cgccagcgag aacgggatga aggagtacca gtcgaacgcc agactcaggc
     9601 cgatcagcaa gacgccggct accagcgcca gatacagccc gacgaacgcc ggccaatggg
     9661 tgcggatgta gcgtgtcgtc cgccggaatt ttggcttagt ggacatgcgc ccgaccatca
     9721 atcataatca ttgtattcat cggccgctat tgtacggttc ccgcggccgg gaggcatcta
     9781 tcacgcacag aggcccgcca agtgagccgc cacggcgcct gtcgggtttg gaatgcttgc
     9841 acaatgacaa tgatcgataa accagacccc tatagtcgct ttctcgaccg ttatgaggcg
     9901 gggccggtcc cctgggatga tccacagccg ccaccggaaa taattgcgct ggcggccgaa
     9961 ctggagccgg ggcgggcgct cgatctgggc tgcggctatg gtcgagtgtc gatctatctg
    10021 gcccaacgag gttggtcggc cgacggagtt gatttcatcc cacgggcgat cgaggttgcc
    10081 caagagcgag cggccgctgc cggagttgcc gattcggccc gattccacgt cgcttcagcg
    10141 gccgaattgg gcttcctgac gccgccctat gatctggccg tcgatatcgg ctgcatgcac
    10201 tccttcaacg aactgatgct gcgcggctac cggtcgggat tgatgcggtt gctccggccg
    10261 ggcgggcttt acgtgctgtt cgctcatctg cgcgacgaag acgaacctgt gatcgatgac
    10321 ggcccgcggg gcataccgga gcgcgttatc agggagttgc tcgtcgatgg ctttgaactg
    10381 gagcgggttg agcatggcgt cacgcaggtg gaaggccgcc cgccgtggaa ctcgggctgg
    10441 ttctggtatc ggcgtcgcta aagaggtgtg ctcatccctt ttatatacgc cccaatacat
    10501 catcgtatag cggcgccatc gtgccccaat cataccgtgc tgtgtgagga gccagccgtc
    10561 gggcgacgtc ccgcgccgcg gccgaatcgt tcagcgccca gcgcaaacgt cgcattaggt
    10621 ccggccgacc atgataaagg caatcggcat ggaacgcttc cggtagcagc tccgggtagc
    10681 taagccgtgc cggcagcaac gggaaagtgt ggcagtaaat tgcttccagg atggagatgc
    10741 cgaagtattc atggccggcc gttgagacgg tgacggccgc ttcccatagc agatgccggt
    10801 agagatcgtc gtcggcgtag ccgttgtgga tcacgcgctc gcccaattcg gccacgcccg
    10861 attcccaacg ctcgccgggc ttgccgaacc gctctccgca aagcgccacg cgaaagggga
    10921 tgccctcggc cgccagcgcc accaggacgt tgatgaaccc ttctggattc ttgtcgtatt
    10981 ccagccgctg attccacaat acgagaggcg gatcggacag aggcgcgtca cccgactgca
    11041 aatcgagacg gcgtaaatca acgcccacgt gcagcacgtc gcttttttgc cgcaaggagg
    11101 cgatcgattc catctcgttg tattcgggat aatggcgcag aaaagggagc agggcagtga
    11161 agaaggtgtc cagatgatat cgggagttga agaaaacccg gtcggcggcc agcatcgaga
    11221 tgaagttgac gaaagcgtaa tgccgatcgc gctcgcccag ttgccggcgc atggggccga
    11281 tcgtcgggtc agttggcaac ggataggtca gctggttctc gtgcatgtag agcgcaacgg
    11341 ggattgcggc cgtttggcgg cgagtcagag ccaggaatgt cgtcaggtca tgcagatccg
    11401 tagagaggat cagatcgggc gcggggctgt tctgtgccaa ccagtggcga gccagggtga
    11461 cggccccgcc atgcatgcgc cacttccaaa aatgggccgg cagggtcagc agatcgacct
    11521 cgtgccggct ggccgaccga tatccttcgg cccaggcgcg atggctgccg ccgtgatagg
    11581 gtgaaagcag gagaatccgc atagatgcca aaatatgtgc ccgaatttgg ccgaactaaa
    11641 ggaagatatt cctcgttaca attcgaccat ccgtctacaa ttgactatac ttgtaaaagt
    11701 tggtcattcg ggacaaacgc tttgtctgag aacgaaccag aactttactg ggattccact
    11761 tacgccatcg ttgtctccct gatggagcat tacccggaac gcgggcctat tgacgtaggc
    11821 ctggctgaac tggtagagat ggtcgaatcc ctgcccggtt atcaggacga taccgccctg
    11881 gtgaacgaac agttattgtt ggatatttta accgtttggt atgaggaggc tacatagcta
    11941 tgaccgtagc gaatacgggg accgccggcg gggacgttgc tgtccctgat gaactggcaa
    12001 acaatgtctt cattaccaac atcgccaagc ttctggaccc ggtctacaac tggtcacgac
    12061 gtaactcgat ttggccggtt acctttggcc tggcctgttg cgccattgag atgatctgca
    12121 cggcatcgag ccgttatgac ctggcgcgtt ttggcatgga ggtattcagg gccacaccac
    12181 gtcaggccga cttgatgatc atctccggga cggtcaccaa gaaaatggtc ccgaccatcg
    12241 tgcgccttta taaccagatg cccgagccgc gctacctgct gagcatgggc gcttgcgcat
    12301 ccggtggcgg ccctttcaag gaaggctata acgtcgttga cggcatcgac aagttcttgc
    12361 cggtcgatgt gtatgtgccc ggctgcccgc caactcccca ggccctgatc aacggcctga
    12421 tcgccctgca ggaaaaaatc gaccagcagt cgatcgcgac atctccctgg tataacaaga
    12481 aggatcagac tgccggcctc tatccggtgc cggtgctggg gcctgatctt gtggacctgc
    12541 ggcaactcaa tatcatccgc caggaagcgg ccaaatatga ggcggagcaa gcggccgccg
    12601 aggtaccctc ggctgaggcc gaaccggctg cctgagcggg cacagttgat tgatattcat
    12661 cggagaagtg atgagcgata caactctcga atcgaccgcg gaagccccgg aaaccggcgt
    12721 acctctgacg cctgcccagg aggcgatcgc aaaacttaaa gatttacacc ccaatatcgt
    12781 cagtgacgac gctcgcgagg gatatgaagg cctggttgtc tatgccgact ctatcaccga
    12841 agtggccaag tcgttgcggg atgatctggg tttcaactat ctctccagcg ttaccggcgt
    12901 tgacctcatt gaagatggaa aaatggaggt ggtctatcat ctctatagca tcagccgtgg
    12961 cggcggccct gttgtcctgc gggcccaggt cgaccgcgat gcgccgtctc tgccgtcact
    13021 ggtgcctgtt ttccccggcg cggagtttca ggagcgcgag gcctacgata tgtatggctt
    13081 ccagttccag gggcatcccg atctgcgccg catcctgacc tgggacggct ttaatggcca
    13141 tccgctgcgc aaggactgga aagaagcctt ctttgaggaa gaatacaagc cgttcggcag
    13201 tcgctggccc gatggtggcg gcgtcgcccg tgctgaagag cacaaccctt acggcaaaaa
    13261 tgtccagtat ccaccggggt ggctccccac cggtgatgag tacgacgtag agacagacgt
    13321 ctatctgggt ctgtcctatt cgcgcgacac gactcccgga ctgaagaccg accgagtgac
    13381 cgtcaacctc ggcccacagc acccctcgac ccatggcgtg ttccgcatgg tcgtcacatt
    13441 ggacggcgag acgatcgtcg atctgaagcc ggtgatgggt tatctccacc gcaatcatga
    13501 gcagattggt gagcgcaata cgttcaacat gaacatgccc tacaccgatc ggctggatta
    13561 cctgtcgtcg atgtccaaca acctcggtta cgcgatcacc atcgagaagc tgttcgatat
    13621 aaccgtgccg gaacgggcgg aatggattcg catcctcatg gtagagttga cgcgtatctg
    13681 caaccacctg tgggcgcttg gcttcctgct caatgacctt ggggcattgc agaccccgat
    13741 gctttacttc tatctggagc gcgagctgat cctcgacttc ttcgagtctg cagccggagc
    13801 acgcctgctg tgcaattaca tgcgcttcgg tggcctggcc tatgacctgc ccgacgatgt
    13861 gcgtggcgtg gagacgatga agttcctcga ggaactgatc tacaaccgct tgccaaaggt
    13921 gctcgatcag ggcgatgact taatgaccgg caacgagatc gttcgtgccc gtgccattgg
    13981 tgtcggcatc ctcacgcctg aggacgccat cgccatgagc gcggccggcc cgctgctgcg
    14041 cgccagcggc gtgccttatg atgttcgtcg cgcggagcct tattcctatt atgagcatct
    14101 cgattttgat gtggccgttc gctataacgg cgacatctat gatcggtatc tcattcgtat
    14161 ggacgagatg cgccagagct tgcgcattct ggagcaggtg ctgccgcatt tgagacagac
    14221 agccggtgcg cctatcatca acgggaagcc ggagtacgcc ttccgcgcgc ctcgcgcggg
    14281 ggcatcttac ggccgtgtcg agaacccgaa gggcgagctg ggcttttacg tcaccgccaa
    14341 acgacgcgcc ggcaacccgg atcgctatca cgtccgggct ccatccttca tcaacctgac
    14401 ggccttaggc ccaatggctc gcggccacaa ggtcgccgac gttgtcgcca tcctcggcag
    14461 cattgatatc gttcttggcg aggtcgatcg ttagatagtt catgtttgtc taacgaaacg
    14521 ggagaattca tgtacggatt aggtattgtc aaagggatgg gggttgtcct ccgtcatttt
    14581 gttcaaagct atgtggatga cttaaagtgg gccggcaagg gcggccgcta ctataacgaa
    14641 gaagcctttg cggttcgcca agggccgatg ggcgagggcg ttaaaacagt cttctaccct
    14701 gaggaaaagc tgtcgacacc cgaacgtttc cgctttgtgc cgtttctggt gaccgatgat
    14761 ccgcctccgg gccagcgctg gggccatgac tggtgcacgt catgcggcat ttgcgccaag
    14821 gcctgcccgc cacagtgcat ttggatcgag cgcggcaagc aacccaatgg gcggccgaag
    14881 ccggagccgg agaagttctt catcgatatc gatatctgca tgaactgtgg ctattgcgcc
    14941 gagttttgtc ccttcgacgc gatcaagatg gatcacgact acgagttggc cagctacgat
    15001 cgtcgcacgg cgcatatcca tgacaaggaa cgtctgagca agccgctcag ctactggcgc
    15061 gaaattgccc cgaagaaggc ggaagcggaa gagcttgcgc gccagatggc cgaagcggcc
    15121 aagaccaaga agaaggataa ggacggcgag acgcgggacg cgcgactggc tgaggcagtc
    15181 agccgccagc tatattatca gggtgcgcga tactaagacc gagccaggcg atgaaggaca
    15241 agttggaggc gtgccctcct atcgtggatt cttgcgcctg cccggccttc tatagcaacc
    15301 tgagcgacct gtgcggttcg ccatcgaaaa aatagcgccg ctgaaatcgc tccccccgga
    15361 gcgatttcac tttcaggcga tttggagagt tgagccatgt ttggaaacaa aaaagataat
    15421 ccgggcgtga cccaggaaac ggtgatgaag gctttatcga ccgtgattga gcctgatttg
    15481 catcgcgatc tcgtctccct caatatgatc cgcaatttgc agatcgacgg aaacgatgtg
    15541 accttcacta ttatgctgac gactcccgct tgccctttgc gcggcaagat ggaaggcgat
    15601 tcccgcgccg ccctggcccg cgtgccgggc atcggtcagg tgacgatcaa ttgggattcc
    15661 aatgtgccgg ccgatcgccg tatcggcgaa caggttggcc agaattttcg taacaccatc
    15721 gccgtgtcca gcggcaaggg tggcgtgggc aagacgacgg ttgccgtcaa tctggccatc
    15781 tcgctggcga tggaaggggc gcgagtgggc ctgctggatg ccgacatcct cggtccaaat
    15841 gtgccgatga tgatgggccg cgaacagatg cccacgccgg tcaatcgcaa gatgatcccg
    15901 gccgagaatt atggggtgaa gttcatctcg atggcctttc tggtgaagcc cgatcaaccg
    15961 ctcatctggc gcggccccat gctccacagt gccatccgtc agcttctgac ggacgtcaat
    16021 tggggtgagt tggactacct catcgtcgat cttccgccgg gaacgggtga tgcccagctc
    16081 acgctggctc aggtgttgcc tctatccggg gcgctggtca tcactcagcc gatgcaggtg
    16141 gcggcatccg acgccgtgcg tggcttgaag acgttcgaga agctcgaggt gccgatcatc
    16201 ggcgtcatcg agaatatgag tggcgacttc ttcggtaccg gcgcgggtga acagctggcc
    16261 aaggaatacc agactaccta tctgggatca atcccgctgg aagctgaagt gcgcaagggt
    16321 ggtgatagtg gccgcccgat tgtagttgcc catcccgatt ccgaggcggc caaggccatt
    16381 cgccgaatcg cgcgcgatgt cgccgctcgg gtcagcgtgc ttactttgca ggtgcaggcc
    16441 gataatatca ttccgatttc gatgatcggc tgatacgatt tttggcttgc tggggggcga
    16501 gtggtcgcgc cgcggccgct cgccttttgt tttgacgggt tgctcaggtt gctctgtttc
    16561 cggtataact tggaactgga tatcctgact taacatgagg aacaggtcta tgaccactgt
    16621 gatggctccc ggcgaaacct cggactttat cggggttcgc tttcaaaagc tgggaaagct
    16681 gtaccacttc aaggtcggct cccataaaga tgtagttgtc ggcgatcatg tgatcgtcga
    16741 gaccaaacgc ggcaaacaac tggggcaggt gatcgccttc attgatccgg aagagatcca
    16801 ccggcagaaa ggcctgcgcg ctgttcagcg caaagccacg ccgcgtgatt tggtcatgaa
    16861 gcaggtgtgg gaatccaagg aactggatgc gcttatcgcc tgccgagaag aggcgtccaa
    16921 agccgacatc aaggacgcca aattcgtcaa ggcggaatat agtttcgacg gctcctggtt
    16981 gacgattgcc tacacgaccg agaacaagaa acttgacgtt cggcaggtgc agagcgcatt
    17041 ggggcgcatc tatcgcacgc gagttgagat gcagctggta gggccgcgcg acgtggccaa
    17101 gatgatgggc ggctacggcg cgtgcggcat cccgcgctgc tgctccacgt tcctgaccga
    17161 cttcagcccc atctccattc gcatggccaa ggagcagggc atctcgctca gcccgcagga
    17221 aatcacgggt atgtgcggcc gcctgcgctg ctgcctggtc tatgaatatg agcagtatgt
    17281 cgaggccaag aagcatttgc ccaaggtcgg caagactatc ggcacgccct atggggaagg
    17341 ccgggtctac gatgtgcggc cgttgcggga ttcggtgatg gtagacgtcg gcgaggagtc
    17401 ccacgaagtt ttccgccacg aaatagagcc gattgaagag ttggaagccc tcaagaaaaa
    17461 ggccgaagcc ggttgctcga agcatgaaaa cggtggctgc gactgtgggg cgaagcggcc
    17521 gaagagccag gatgatcccg gcgaagggga atagcgtgaa gacgagagcc gacgcgtggg
    17581 atacggtgtg ccggtatgtg caggagcgtg gtctgcgccg gcatatgttg gccgtaagta
    17641 tagcgatggc ggattatgcc cgccggttga acgaagacgc ggcttactgg gagacagtgg
    17701 gcttgctcca cgactttgac tgggagatcc accccaatct caacgagcat cccggcaagg
    17761 gggcggatat ccttcgcgcg gaagggtggg atgaagagac gattcgcgtc atcctttctc
    17821 attataccga aggcaccggc gttgaacggg aaaagccgat cgattttgcg ctcctggcgt
    17881 gcgacgaaat taccgggctg attatcgcca caaccctggt gaaggcatcg cgcaatatcg
    17941 ccgacgtgac cacagatacg ttgcgcaaaa agtggaagga tcgccgcttc gctgccggag
    18001 tcgatcggga tgctgtcgct gctgaaacgg ccgattttag ccgtgtctcc tttgacggca
    18061 ccctcgaatt gtgggaccac atcggtaatg tcctcggggc gatgcaagca gcggcggccg
    18121 aacttgagct ggacggccgc ctggcagttc aatagcgctc catagaagtt tgccctatgg
    18181 caaaacagga agacctctct gttcgtgaag ccctgaatct aatactgggc accatcgata
    18241 ttctatcgtc tgaagaatta aacttgttcg atggcctcgg tcgagtgctg gcccaatcgg
    18301 tgaccgcaca tgatagcctg ccgccgttcg ccaactcgtc gatggatggc tacgccgtgc
    18361 gtgccgagga cgtcggcggg gcacgagaag atcgcccgat ccgcctgaaa gtcatcggcg
    18421 atatcgccgc cggtgcgggt attttacctg aagtgatcgc ggggacagcc gcgcgcatca
    18481 tgaccggtgc gccattgccg cccggggctg atgcagtcgt gcctgtcgaa gataccgacg
    18541 agccatggcg cggcaaagaa cggcctttgc cggaatttgt ggcaattcat cgggcagtag
    18601 gccctggcga cttcacgcgt caccccggtg aggacattcg cgcaggtgat attgtgttag
    18661 agaaggggtg tgtattgcgg ccgcaggaga tcggtattct ggctgcttta ggtgtgggtc
    18721 gaattaacgt cattcgtcgg ccgcgggttg gggttttggc cactggcgat gaattggtcg
    18781 atgttgcttc tccgctaacg ccggggaaga tccgcaacag caacggctac gcgcagacgg
    18841 cacaggtggc tgccctgggc ggggtgccgg tgatgctggg tgtcgctgga gacaccgagg
    18901 cggccgtccg cgagcggctg gatgccggcg tcgctgccgg ggtcgatctg cttattagct
    18961 cggcgggcgt gtcggtcggc gcttatgatg tcgtgaaagc ggttctcgat gaagtgggtg
    19021 atgtcgcgtt ctggcgggtc cgtatgcggc cgggcaagcc attggccttc ggctcatatc
    19081 ggggcacccc ctttttgggt gttccgggca atccggtgtc ggccatggtc tcgtttgaga
    19141 tgttcgcccg cccggcgatc ctgaagatgg ccggccatga caagctggaa cggccgcggg
    19201 tcgaagtgac tattttggaa gatatcgact cggatgggcg agagagctac atccgggcgg
    19261 tcgtcacccg cgacgagcgc ggctacctcg cgcgcacgac cggcgggcaa ggttcccata
    19321 tgatgacttc gttggtgaag gcgaatgcct tgttggtggt gccggagggg gtaaaggcga
    19381 ttcaggctgg gacgaagttg aaggcgttga tgatcgactg gtcgggaggc attttctagc
    19441 ctcccgatag ttcgatccgc ttgtcgtggg cgactctaat tgtcgctttg cattgtcagg
    19501 gtgcgcttga gaagatcgtc ctgcacacgg cgttgatctt cgcgcactgc cttcttgccc
    19561 ttgcgcccgc cacgctgctc ggtcttgacg gccgagatgg catcgttgcc cagcgcacga
    19621 cgcatggcga tttccatggc cgtaggcact tcctcggtct catcctcgat gatttcgtaa
    19681 acttcaggct cgaagcgctc gcggctctgg cgacgtttgc caccaccgct gttctgtcgc
    19741 ggttgcgagg gctgcatggg tgccatctcc ggtttttcga caagtgcctt catgctcagg
    19801 tcgatgcggc gcttgcggcg attgaagccg agcaccttga cctgcacttc gtcgcccacg
    19861 ttgaccactt cgctggggct tttcacataa ttgtggctca gctcgctgat gtggacgagg
    19921 ccttcgcgct cggcgccgat actgacgaat gcgccaaagg tctccagacg ggtgactttg
    19981 ccctcataga cctggtcggt cttcagctcg gtccagtcga cggccagcgg gggaaccatg
    20041 gtcagcataa cctgctggcg ctccgggtcg accttttcga cccagacagt gacttcgtcg
    20101 ccctcgttca gaacgtcgga aacgcgattc acttggtcgc cgcccagttt ggatatatga
    20161 atcaagccgg tggcgtcgat acccaggtcg ataaacgcac catatagctc cagccgcttg
    20221 accgtgccct tgagctgcat cttgggcgaa aggtccgcga tgctggtcgg cgcggcctcg
    20281 gtaattattt cgggggtcat ctcaatagtt tctgcactca tgacagaact ctcctgcata
    20341 ggctttgaat atagcgccga ctgaaccgga cgcttggtta gctttgtgcg ttgttcgacg
    20401 gggggtagaa cattgctagg tgttaactcg atagggtagg attataacaa tcgagccttt
    20461 tgtgtcaacc attttatgac gattccagcg gctaacctca ttcaaataat cctaccctcg
    20521 ttcgcttaca gtcgatctgt cgtcaatcgg gaaactagaa ctcaacctct tcggaatcac
    20581 ccccatcagc tgcgacggct tcgactgcaa tggccgtgat gtgggattcg ttgcgaatac
    20641 gtcgttcaag ctcaagcagg acggtggggt tctcggccag aaagaccttg gcattctcgc
    20701 ggccctggcc caaaagcatg tcattatagc ggaagtatgc gccacgtttg tcgacgatat
    20761 tgtaattggt ggccaggtcg agaacatcgc cggtcttgga gataccttcg ttatacatga
    20821 tatcgaactc gcactcggtg aagggtgggg cgaccttgtt cttcttgacc ttgacccggg
    20881 tgcggttgcc gataacgtcg ttgccggcct tgatggcctg gatgcgacgg atgtcgaggc
    20941 gaaccgaagc gtagaacttc aaggcattac cgccgggggt cgtctcgggg ttaccgaaca
    21001 tgacgccgat cttttcgcgc agctgattgg tgaagataac gacggtgttg gtctgcttga
    21061 tggcgccgga gagcttgcgc agggcctggg acatgaggcg cgcctggagg ccgacatggg
    21121 tatcgcccat ctcgccttcg atctcggccc gggggacgag ggcagcgacg gaatcgatga
    21181 cgacgacatc catggtgccg gaacggatga gggcgtcggc gatctccaga gcctgttcac
    21241 cggtgtcggg ctgggagacg tagagattat cgacgtcgac gccgcacttg gcggcatagg
    21301 aagggtcgag ggcgtgctcc atgtcgatga aggcgcagat gccgccgagc ttctgggctt
    21361 cggcgacgat gtgctggcag agggtagtct tgccggagga ctcggggccg taaatttcga
    21421 tgatgcggcc gcgaggcacg ccgcccacgc ccagggcgat gtcgagagaa attgagccgg
    21481 tgggcacgac ctgaatgtgc agattgtggc cttcgccgag gcgcataatg gtgccttcgc
    21541 cgaaccgctt ggtgagggaa tcaatggtct tatcgagggt ggcctgacgg ccgtcctggg
    21601 tattgctgcc attagagcga gacattgagg taactcctgt tttgatagaa agccggatcc
    21661 acgctgtttg aattgactaa gcgcataaca atgacagtag aatagcacgg atgttcgtat
    21721 ctgtcaatat ctagtttatg aatatgaacg attgttcaat attagaaaga aggctatgtg
    21781 gaaatggttt ttgagcgggc gatttgcagg agcgagcgca gttcgcgacc acccaatagc
    21841 caggttacga ccagatagac cgagccgcca ataattagcc cggcaaacag gaagggaagg
    21901 gtgccgctga cgacccggcc gattagccaa attgccaggc tcatggcggc tgcgccaata
    21961 gtagcccgaa ggaaggattg ggccagcggc cggccaccga ggccgttgag gtcgcgccgg
    22021 ttcaattcga agaggacgat ggccagaaga gtaaacccga cggccgaagc caatgccagc
    22081 cctaccacac ccatctgacg aactaacagg aatgaaatcg cccctgtcac gacgaaccag
    22141 ccgatgtagg ccagcatagg cgtaaatgtg ttatggcgcg cgtagaatag ccgggccacg
    22201 atctcaagtg tggattcgct gacgatgcgg atactcaata tggccagcac cgcgtagacc
    22261 attcgggtcg cttgttcatc gaatgcaccc ccctggaaca gggtttcgat aataggacgg
    22321 ccgagaagaa cggtcgccgc cgcggctggg atggtcagcg tccagatgat ggccagggtg
    22381 ctgctcgcgg tgcgtttcag tccctcgatg tcgcgggcat tataaagctc cgccagagtg
    22441 gggaacacaa ccagggcgat ggccgtgccg aaaagggtct gcgggaactg catcaagcca
    22501 tagccataga aatagctgga ggtagagccg ggcggtaacc ccgacgccag acgaatgatg
    22561 atcaggtcgg caatctggat gacacccagc atcacaa
//
LOCUS       DAOMQG010000017        22372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_993_length_22372_cov_9.290093, whole genome shotgun sequence.
ACCESSION   DAOMQG010000017 DAOMQG010000000
VERSION     DAOMQG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 22372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22372
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_22372_cov_9.290093"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLC06_02200"
     CDS             complement(<1..792)
                     /locus_tag="PLC06_02200"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO89285.1"
                     /translation="MNDKREITGWMMYDWANSAFSTTVVTALLGPYIKDLVEAATEPF
                     RVFGTAIQPGAIYPAAASLSVILQVFFLPLLGTIADYTHLKKRMLLAFAYTGAFATIL
                     LFFVRADMPAIGTRGAVVVGSLLFIIANLAFGAAIVFYNSFLPQIASPDKRDEVSSKG
                     WAVGYLGGGLLLLLNLLLLMVMEDTALAMRISLASAGVWWLVFTFLFPQKRLIQRAPS
                     RSRADVNLFTHGIKQLSNTFSELWHRYPMTLRYLIAYLVYNDGIQT"
     gene            complement(929..2467)
                     /locus_tag="PLC06_02205"
     CDS             complement(929..2467)
                     /locus_tag="PLC06_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRO89286.1"
                     /translation="MFLAIVLLAGLLLTACNAGQPTAVVTPFPTGTAKGKSTAMPEPA
                     TPTLEATEAPTDVPATEEPIADVTEAPEGTDTPRPSPTVRPSPTRRPTATPTSLPQAN
                     PTAALNITGSMREGEPTPATAIPSPVPIFELPDGTTNILLLGKDQNADGSDGRTDTMI
                     IVSINRETRTASMISLPRDLYVYMPNRVMSRLNTAVTLGGVELLEQTILYNFGIPIHY
                     YAQVDFEGFKRIVDLLGGVQMAVSCPLEDWRLISPELDPALEENWERFKLEAGMYQMD
                     GDLALWYARSRLTTTDFDRGRRQQQLLQGMLNQSVDLGLVAKAPELWSTFNSVVETNM
                     DIGRILQLAAMAPDVRANGIQHLYLAGKMQAWSVPESGAAVQLPIWEGEDMMKDTFRR
                     LLLPPALNRADRAPITVEIINASQYPAQGLLAADNLAWYGFVPVLGETREPQDNTEMF
                     YFGENFKGSYDWLFSWVMGKRMADIQLVDEPSEFNYRVIIGNDYNPCRPAFDAPQSSL
                     PPES"
     gene            complement(2516..3418)
                     /locus_tag="PLC06_02210"
     CDS             complement(2516..3418)
                     /locus_tag="PLC06_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89287.1"
                     /translation="MSDQSRPRADGEMPTYTYNADRGRFDWRDKLRELWHYRNLLRNL
                     VARDLKARYKNSVLGILWSILNPMFLMLVFTILFSVLANNQIRDYPIFVLTGLIPWNF
                     FSGSLISGTVSISSNSGLVKKVYFPRELLPMSALLSNLVNFLFAFLVLIAFLYIFGIG
                     LTVHALWVPLILITQLIFTLGLALLLGSLTVFYRDVLMILEVVMLAWFFLTPVFYSLE
                     MFGNTATVLGYTFYPAQLLRWINPMASIIDGYRTVLWGTYDSGGPVSMYSPYLLRTFV
                     TSIIVFILGYAVFNRLNPLFGEKL"
     gene            complement(3484..4788)
                     /locus_tag="PLC06_02215"
     CDS             complement(3484..4788)
                     /locus_tag="PLC06_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89288.1"
                     /translation="MRILHVVHQYVPDHVAGTELYTQSVARRQAKAGHEVAVFTPLNR
                     DSHFSAQPAVEEGVRVYRVPAGRRSPTAVFRSTFGHAGLQAAFDAVMAREQPDVVHLQ
                     HLMGIPAAIVDAIRQSGAPYVISLHDYWYGCANGQLLTNDTKRLCDGPDSRFHNCGRC
                     AVARAGLVPVAGLLGPVAAPVLRRRDALLRPIFVGAGRVLAPNEFVRNIHATMGFSTD
                     HVVINPLGLDTPPELIQHLAAQRETRPTGNIRLGYLGSLSPQKGLHVLIEALNGLPDS
                     ISLDIYGDPAVFPDYVAEARAMNRHPGVQFNGLLARDRLWETLGDLDALVMPTLWYEA
                     SPATIREAFAAGLPVIASNLGAPGSMVRDGVDGLLFPPGDAAALREILRGLIDRPERL
                     ADLRAQISPVRTEADHVAQIEDIYRVTLTLGNHGSAIGPNTL"
     gene            complement(4796..6067)
                     /locus_tag="PLC06_02220"
     CDS             complement(4796..6067)
                     /locus_tag="PLC06_02220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89289.1"
                     /translation="MNDQPLHVALVSPAYPPLPGGGERYVGALARALVAAGTKVTVVT
                     SSATAEADFWNGVESRSDGDEDGVRVIRLPILPAAGGRDALMRRRRLMVLLSAMPGDQ
                     SSMLAGMARRFPAIDGLDETLDGLRGVDVVHGFNISWEHGLVAAHEYSRRAAVPLVAT
                     PFAHLGESSLGRVARNSTMNHQLRLLRDAARVLVLTRLEADGLARYHVRPERLDVIGS
                     GVDPIPDDYTESPYFSLEHHEARGDFGVYIGRQSFDKGALHAADAVRLLRQRGRDIAL
                     LLIGSAAPEFEKYRRRLSPEDRAAIRPLGILSDRDKHAVLSRAQFLMLPSRADSFGIV
                     LLEAWSHGVPVVAARAGGIPGVVDDGANGLLVPFADVDGLANAVERLITDGAFAERVG
                     EAGREKIRTHYSWDVVAARALEQYRRITGTG"
     gene            complement(6067..7152)
                     /locus_tag="PLC06_02225"
     CDS             complement(6067..7152)
                     /locus_tag="PLC06_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89290.1"
                     /translation="MPERVLHQFTEGAYVGDAVSDQAFLIQRWLRELGFVSELYAERI
                     QPELAGRVLPAGEYRPRSVETCLIHHHAVGSIIAGRLMKIGLPQILIYHNITPPEFFE
                     QTNPALADSLKRGRQQLAQMRPLVLLGLGASIYSEGELISLGYTPTGVLPIVLDESSY
                     SISSNDELVRRCDNGRPLLLFVGRFMPNKRQEDLIKLLYYLRRARPGARLVLVGNTDN
                     REYVDWQRALARDLGVDAAVAFAGHVSHQDMITYYRAADLYVSMSEHEGFGKPLIESM
                     YCGLPVLAYASSAVPDTLGGAGVLFHRKDYEALAELVDLFLEDEGLRRRVIDRQFSRV
                     QAFLEPRVREQFIGYLQQLNLLPGVSP"
     gene            complement(7145..8338)
                     /locus_tag="PLC06_02230"
     CDS             complement(7145..8338)
                     /locus_tag="PLC06_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89291.1"
                     /translation="MSGKKRVAMVVQRYGEEVNGGAEMLARWVAEHLTALAEMHVITT
                     CAVDHHTWADVYSPGESELNGVVIHRFRVDGLRTDLHHNELFGGHRSIFTQVHWMKEQ
                     GPYSSDLLNYVYDSYDEYDLYIFVTYLYPPVFFGLPLVSDKAVLIPNAHDEPYLHLPI
                     IRSLFHLPQMIIYNTVPEKLLVNRVMHNHYVPQIVAGVGVDVPADVSAARFRGKYGID
                     GRFILYVGRVEESKNVPELFDHFIRFREETGADLKLLLIGKVKVYVPDRPDIVPLGFV
                     SEEDKFDAISAADVLVMPSLFESLSIVAMEAWLMGTPTLLNGRCEVLEYQSRQSNGGL
                     YYYSYEEFASALTMLLAHDDLRAQLGRQGRAFVTANYSWDVILAKYRAVLETLTDMPH
                     RGDDA"
     gene            complement(8335..9402)
                     /locus_tag="PLC06_02235"
     CDS             complement(8335..9402)
                     /locus_tag="PLC06_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89292.1"
                     /translation="MNRGPLHQFLTGATAGDAITGQALLIQRWLREAGYESEIYAWHL
                     HDSMATAARPMSTYRRARGETRGIYHHSIGSDVPEFLAGRDLRLILIYHNITPPGFFE
                     NSDPLRAYLARQGIAQLKMLQPLTELALADSHYNERDLLLNGFIRTAVLPICLQAEQY
                     EAAVNGTLPETPAGAEPRLLFVGRLAPNKRQEDLVKLLFFLRRIHPQAHLTLVGDRWE
                     MGYDRWVEQLAADLNVAEGLELTGKVSHEALLAHYRSADLYMSMSDHEGFGVPLIESM
                     LLGLPVMAYGSTAVPETMGDAGVVFYRKEFEPLAELIAMLYEDATLRRRIIGKQRARV
                     EHFLEPQVRRQFFSYLEQIEA"
     gene            complement(9426..9911)
                     /locus_tag="PLC06_02240"
     CDS             complement(9426..9911)
                     /locus_tag="PLC06_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89293.1"
                     /translation="MESQHAADSPWIVIHDEQVSSEELVRQVEERVARRRAELGAVEV
                     VFPAFGYVSSYPDVPLEKRVSPHLFYYLEQANRMPPASVEPLLAPSPATRAPVVGGLW
                     QKIRGEMHNLVLFYVNKSVREQSRTNANLVSTLNELTRVIQDQQAEIDALRAEIWRRE
                     N"
     gene            complement(9917..10504)
                     /locus_tag="PLC06_02245"
     CDS             complement(9917..10504)
                     /locus_tag="PLC06_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89294.1"
                     /translation="MNQNSSGQRTEEPVNIEHVMMEVRREILERKLPGQVRLPEAAAG
                     SQRPEYYEELFRAALAQSRNEVDLLVTPTRVPLVGPVVDFVRRKFHELVVFYINRSAM
                     NQAKVNHHILAALSILGQADPAETPALGEMASVNQDAPVTLDDVRAGYRLFFGDGPEA
                     ADLDYWTNLLATERVTRDRLIESFLNTRPAGRETK"
     gene            complement(10491..12674)
                     /locus_tag="PLC06_02250"
     CDS             complement(10491..12674)
                     /locus_tag="PLC06_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89295.1"
                     /translation="MVSWLGVLLLLILAALTGVLLAARFIPRGSRWNLPTAFAALTLG
                     VISVGWVALILAELGVFSLPALLVALAVPVGLLGFWRWRGAGKGDAAGEYASPEDRLA
                     RWEWAMLAVWLAAALWLYFRPHEYVFGAADAGVYISLGAEIAQEGGFRVTDELLAGMT
                     PGMRAAVLRPLPNTPFADSYYLPGFYVTNEAAGQLTPQFYPLHPVLQAIVYGFAGGGA
                     SGVRAELLLTGLWIMLGSLAVYLTARDIGGPVTGALALAGLSLSGLQVWFGRYPTSEA
                     LTQFLLWAGIWSAGRWLEERRPAALWAFVSGCAFGAVFLVRIDSLVLLPVFGIVLLWR
                     WLRGWQRLDSWFAAPFGVLVIHSLLHGHFLSAPYFYETVGYGFILLGRLWPFLLGGLL
                     ALALLLLWLPRRVGRYRAPDHVQRIVRYVLLTLLFAYALYGWFLRPELNITTLRPDTF
                     SAGEIPVTNHENWIRLGWYLSPLGIWLGVLGAGLMLWRVNRRSVMALALGMLFAGVYL
                     WNISANPHHVYVMRRYVPAVMPFFILGAAYLLGHLGFRRADWDRAAPGFLRRLPHPAG
                     PLLVAAVTVGWLAGLGWSARGFVSQVDHAGLTTQIDALAAGLPPDAVLLFNDQSPVGE
                     GDIWGTPLRFIYDFGVFALRQSPDTISPVLVETIKTWQNNDRTVIWVGPANWLDEQGL
                     DYRAEQVTLSRRRLESSYQHKPYRVDTEVSVLTLNYLEPVTNESK"
     gene            complement(12667..14196)
                     /locus_tag="PLC06_02255"
     CDS             complement(12667..14196)
                     /locus_tag="PLC06_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89296.1"
                     /translation="MTDASRSMTDNRPQPDPSNILRQRATLQEYEFNSQAPVVGGLIA
                     RLRRAWNGVATKWQARPVMEQQSAFNRALAERLGRPPGQAEVDDLFARSDRTQTTLTR
                     DAARLSMATRRLSEDKINRRPRIAWFSPLPPSRSGIADYSAELLPHLAELVDLTVFTP
                     DPATITLPGLTVRPVSDFVVNHDEFDLPLYQMGNSDQHEWVYAMLLRYPGAVVLHDYF
                     LHHFIRHRTVGRGEWTAYSRELTYAIGGDGRELARAVRRGSTVSPLFEEPLNQRLIDA
                     SLGLIVHSRYAAERARHYRPDLPLAVVPALVEPRSSHSMRDRLELPDHAILFGSFGQI
                     TTEKRIDVALRAFRDVLERYLDAHYLLVGDPRPDVDLDSLVVRLGVGHAVNRIGYVRN
                     LDEFTDWIRTADVVVNLRQPTVGETSAVALRAMAAARPLIVYDHGWYSEIPDAAAIKV
                     PPDNDEALRQAMEALATSPERRLMMGQAGHDYVVEHCAPSSVAAAYAEFIRDVLGWPN
                     G"
     gene            complement(14189..15976)
                     /locus_tag="PLC06_02260"
     CDS             complement(14189..15976)
                     /locus_tag="PLC06_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRO89297.1"
                     /translation="MNRNQRIASTMTLLLALLGLTLAALAQGGSRAFLPVVQRDAASN
                     GNPIVKINFQPAGAAVPPGYLADSGATYADRGNGFRYGWNADNSAATRERNSANAPDQ
                     RYDTLIHLQKPENPDAVWEIALLPGIYDIYAVAGDPEAWDGYMNSTAEGRTLTDGAPS
                     VDSRFLHGVAGTVPVTDGRLTVRSGPNAVNNKLAFLEIYQIAVLPTPPPGMVEFRGLW
                     VTRFDWTSSKAPASPAKIDEIVNNAADAGFNAILFQVRGAADAYYAPGLEPWAQRVTG
                     GAYGQAPAPLWDPLAYFVQRAHARGLQLHAYINVYPVWDNCTNPPAMVAPTPFYFQLQ
                     GAHGTTDGKLNGVQWTTQHQVSCGDYLYATPASPVVRAHVKAVAADLVNRYDIDGIHL
                     DRVRYAGRTTSCDPVSEAMYGAPCFTNKGLPYDEWQRQQVNTLVREIYQDVVVPAGRK
                     IWLSAAVWATYRDKWGWGYSQGYSDYYQDSRAWVQNGYIDALAPMIYSYKPPAENPFP
                     LDRWRILAADHQANRGGRFIIPGIGGIGPGLLTFDDVVQRIEAARDLGTAGHALYSYG
                     ALQANGYFDDFAAGPYAVPAAVPEITWHD"
     assembly_gap    16215..16314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16348..16992
                     /locus_tag="PLC06_02265"
     CDS             16348..16992
                     /locus_tag="PLC06_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRO89298.1"
                     /translation="MSIRFHDITRTMFAGMSVWPGDAPFELEPTTGIAEGSTVNLTRL
                     SMSAHTGTHVDAPYHFTDDGATMEQVDPAIYWGSAQVVTVKKATGPLVPADFAGYDLG
                     LAPRLLVHSGASAADPRIFPQEFAYPSPKLADYLGALGIILYGTDAPSVDASDSQTLD
                     GHHALRRNNIAILEGLDLTDVPDGIYELAAIPLKILGGDGSPVRAILRTTGANE"
     gene            16989..18263
                     /gene="kynU"
                     /locus_tag="PLC06_02270"
     CDS             16989..18263
                     /gene="kynU"
                     /locus_tag="PLC06_02270"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRO89299.1"
                     /translation="MTSPDFLSREHARALDAADPLPAFRERFVIDDPELIYLDGNSLG
                     RLPRATIDLLDDLVRRQWGGRLVRGWSEGWFDTANRIGDKIAGILGASEGEIIVADST
                     STTLFKLGMAALMARPGRRTILTDDLNFPSDLYILQGMARLAGDDYRVRVIPSADGIN
                     GPAEALAAAMDEDTALVTLSHTVFKSGYQYDMAAITAAAHNVGALALWDLSHSAGSVL
                     VDLNGAAADLAVGCTYKYLNGGPGAPAFLYVRHDLQDTLANPISGWIGQHDPFDFALD
                     YVPAAGIQRFLTGTPPYLSLAAIEPGVDLLLEAGMDRLRERSVRLSEYFIALWEARLA
                     PLGFRLNSPREVARRGSHISLGHDEGLRINLSLIRDVKVLPDFRRPDNIRIGIVPLYN
                     TFAELHEAVDRLVTVVNDRLYERHDTTGLKVT"
     gene            18292..19296
                     /locus_tag="PLC06_02275"
     CDS             18292..19296
                     /locus_tag="PLC06_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO89300.1"
                     /translation="MHPPLRFVTGDGRIIYSFRVRSFPTLVNNIYLIDGGEGLILVDC
                     GSGMPQANAELEAGFAAVSETFGLAVSMADISAILITHGHIDHFGGLNFVRQFTDAPI
                     GVHILDRRVLSNHEERVVFSYRRLETFLESAGVSAELREALMSVYLFAKTYYRSTPVQ
                     FLLEEGKPTIDNIGVYHVPGHCPGQVCLAVDDILLTADHVLSRITPHQAPESITHHMG
                     LSHYLDSLDKIAALSNYRLGLGGHEDPIDDIPARIGAIRQAHDERLSKVMAICREPKS
                     IADISRELFGRVDSYHVLLALEEAGAHVEYLHQRGELMAANLEEIERTSHPVVQYVRG
                     "
     gene            19309..20430
                     /gene="mutY"
                     /locus_tag="PLC06_02280"
     CDS             19309..20430
                     /gene="mutY"
                     /locus_tag="PLC06_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRO89301.1"
                     /translation="MSSHPVAVALLAWWDAGHADLPWRRTHDAYGVWVAEIMLQQTQI
                     ATVIPYYERWMARFPTAEALAAAPLDDVLKLWEGLGYYSRARNLHAAAQIVTHEHGGR
                     LPSTVPELMKLPGIGRYTAGAIASIAFDVPAPILDGNVIRVLSRVHNIEEDVTQSATR
                     TRLWHLAEELVPLKRPGDFNQALMELGQSICLPKNPRCLICPLNTLCLARQHGTQPER
                     PVRPPRKRTPHYDVVAAIIWREGPPGRHAKFLIAQRPHDGLLGGLWEFPGGKVEAGET
                     MADALRREIREEMAIDITPGAFLIAVPHAYTHFRITLHAFHVIYGGGEIEHLGVADHA
                     WVTLDDLDRFAFAVTDRKIIEQLRRTLMPEQPQSQTGAV"
     gene            complement(20389..21252)
                     /locus_tag="PLC06_02285"
     CDS             complement(20389..21252)
                     /locus_tag="PLC06_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO89302.1"
                     /translation="MILITGATGFIGRVLCRALDHTGHAWRAYGGRMNSPEALRAEIE
                     GADTIIHLAGAEARGSDRLLQRVDIEGTQRLIEEARRAGVTRLIYPSRLNANPYSMHP
                     LLRAKGEAERLVRQSGIPYTILRTSTLFGRDDRYSEIVYSLALWTWPLAWLPGGGKMQ
                     MQPLWVEDYVHCLIRTLDRPDMIDRTPVVAGNELVSYREFVRMILNVTGKQRLLLPLP
                     MTLMRPVSRILLGWWYWPGISRYFVDRFFVPDVTDLDVVQRQFGFQPARFRETITYLN
                     RSGLRLRLFRH"
     gene            21300..>22372
                     /locus_tag="PLC06_02290"
     CDS             21300..>22372
                     /locus_tag="PLC06_02290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89303.1"
                     /translation="MSRFSPENPLRVCYFGTFRAGYTRNQIILKGLQAQPDVAVHVCH
                     ATLWQGIEDRVAQASGGWRSPAFWRRVAGAYRQLLREHRQAPEYDVMLIGYPGQFDAF
                     LGRRLAHHRGKPAALDILMSLHLVAEERGLTAAHPNTGRLIFQLERSGLRQPDLLIAE
                     NPEYGGYIAGKYQLPIERFRYVPHGADETVFHPRPLRPPDNYFRVTYHGGYLPSHGMD
                     AILGAARLLADEPGVRFEFYGSGPEKERIVRQAQEWELPNTTFHGFVSQDELLDGLAQ
                     AHVCLGVFGTTRQAHFTVQNKVWEGLAMGRPVISGDSPTIRAALADREEIYLIARSDP
                     AALAAGLHELRDDPALRERIAAAG"
BASE COUNT         4551 a         7163 c         6686 g         3872 t
ORIGIN      
        1 ggtctggatg ccatcgttgt agacgagata agcgatcaaa taacgcagcg tcatcgggta
       61 tctgtgccac agctcgctga acgtgttcga tagctgctta atgccgtgcg taaacaagtt
      121 gacgtcggcc cgggaacgac ttggggcgcg ctggatgaga cgtttctgcg gaaacagaaa
      181 cgtgaacacc agccaccaca cgcccgcgct ggctaggctg atacgcatgg ccaacgcggt
      241 atcctccatg accatgagca ggaggagatt gagcaacagc aacaggccac cacccagata
      301 accaactgcc cagcccttgg aactcacttc atcgcgtttg tcgggcgagg cgatttgcgg
      361 caggaaactg ttgtagaaaa cgattgccgc gccgaaggcc aggttggcga tgatgaagag
      421 caggctgccg acaacgaccg cgcctctggt gccaatggcg ggcatatcgg ccctgacgaa
      481 gaagagcagg atcgtggcaa acgcgccggt ataggcgaac gcaaggagca tccgcttttt
      541 caaatgggtg tagtcggcga ttgtgcccag aagcggcagg aaaaagacct ggaggatgac
      601 ggaaagggag gcagccgccg gatagatcgc ccccggctga atggccgtcc cgaaaaccct
      661 gaacggctcg gtcgccgcct cgaccagatc cttgatgtac gggccgagga gagccgtaac
      721 cacggtcgta ctgaaagccg aattggccca gtcatacatc atccaaccgg taatctcgcg
      781 cttgtcgttc aacgtgtcct cccgataatc gcctgcccgc caaaaaagga cgatggctgc
      841 tccatcggca ggccatcgtc ctcaatcttg tttcgccacg cgggtttcat ggcgaggaat
      901 tatgcccgtt gctcactaaa caagcaattt acgactccgg cggcaatgac gattggggcg
      961 cgtcgaacgc cggacggcag ggattgtaat cattgccgat aatcacacgg taattgaact
     1021 ccgacggctc atccacgagc tggatgtcgg ccattcgttt gcccatcacc caactgaaca
     1081 gccagtcata ggagccttta aaattctccc cgaaatagaa catctccgtg ttatcctgcg
     1141 gctcacgggt ctcgcccaga accggcacga agccatacca ggccagatta tcggccgcca
     1201 gcaatccctg ggccgggtat tggctggcgt taatgatctc gaccgtgatc ggcgcacggt
     1261 cggcccgatt gagcgccggg ggcaggagca ggcgacggaa ggtgtccttc atcatgtcct
     1321 cgccttccca gataggcagt tggaccgccg ctccgctttc gggcacggac caggcctgca
     1381 ttttccccgc cagatacagg tgctgaatgc cgttggcgcg cacgtcagga gccatcgccg
     1441 ccaattgcag aatgcggccg atatccatat tcgtctcaac cacgctattg aaggtagacc
     1501 acagctcagg cgctttggcc accagcccca ggtctacgct ctgattgagc ataccttgca
     1561 acagctgttg ctggcggcgg ccgcgatcga agtcggtggt cgtcagacgc gagcgggcat
     1621 accacaacgc gaggtcacca tccatctggt acatgcccgc ttccagcttg aatcgctccc
     1681 agttttcttc cagtgccgga tcaagctccg gcgagatcag tcgccagtcc tccagcggac
     1741 agctcaccgc catttgcacg ccgcccagga gatcgacaat gcgcttgaag ccctcaaagt
     1801 cgacctgggc gtaatagtgg atcgggatgc cgaagttata gaggatcgtc tgctccaaca
     1861 actcaacccc gccgagggtg acggccgtat taagccggct catgacgcgg ttgggcatgt
     1921 agacgtacag gtcacgcggc agggagatca tcgacgccgt ccgcgtttcg cgattgatgg
     1981 acacgatgat catcgtatcg gtacggccgt cgctgccgtc ggcgttctga tccttgccca
     2041 ggagcaggat gttggtcgtg ccgtcgggca gctcaaagat cggaaccggg gacgggatgg
     2101 ctgtggccgg cgtcggctcg ccctcacgca tcgagccggt gatgttgagc gccgccgttg
     2161 ggtttgcctg cggcaggctg gtcggtgtgg ccgtgggccg gcgcgtgggc gaagggcgaa
     2221 cggtcggtga tggccgcggt gtgtccgttc cctcgggcgc ttccgttacg tccgcgatcg
     2281 gctcctcggt ggcgggcaca tcggtgggtg cctcggtcgc ctcgagagtc ggggtcgcgg
     2341 gttcgggcat cgcggtggat ttacctttgg ccgtgccggt cgggaaggga gtgacaacag
     2401 ccgtgggctg tcctgcattg caggcagtca gcaacaggcc cgccaacagg acaatggcca
     2461 ggaacattga acgaatggat ttcatacgca tttgtgaatg gggcagaggc gacggttata
     2521 gtttttcgcc aaagagcgga ttcaaccgat tgaataccgc ataccccaga ataaagacga
     2581 tgatcgacgt cacgaacgtg cgcagcaaat aaggggaata cattgacacc gggccgccgc
     2641 tgtcatacgt accccacagc acggtgcgat agccgtcaat gatcgaggcc atcggattga
     2701 tccagcgcag cagttgggcc ggataaaacg tatatcccag cacagttgcc gtgttgccga
     2761 acatctccag tgaataaaag acgggcgtca gaaagaacca ggccaacatg accacttcca
     2821 gaatcatcag cacgtcgcga tagaagaccg tcaggctgcc caacagcagc gcaagcccca
     2881 atgtgaagat caattgggtg atcagtatga gcggcaccca caaggcatgg acggtcaagc
     2941 caatgccgaa aatgtacagg aaggcgatca ataccaaaaa ggcaaacagg aaattgacca
     3001 ggttggacag caaggccgac atcggcaaca gttcgcgcgg aaagtagacc tttttgacaa
     3061 gtcccgagtt gctcgaaatt gagacggtcc cgctgatgag cgatccgctg aaaaagttcc
     3121 agggaataag gccggtcagg acgaagatcg ggtaatcccg tatctgatta ttggcgagga
     3181 cggagaacag gatcgtgaac accagcatca gaaacatcgg gttcaggatg ctccacagga
     3241 tgccgagaac cgaattcttg tagcgcgcct tgagatcgcg ggcgacgaga ttgcgcagca
     3301 gattgcggta atgccacaat tcacgcagct tgtcgcgcca gtcgaaccga ccgcggtcgg
     3361 cattataggt ataggtgggc atttcgccgt cggctcgtgg ccggctctga tcactcatcc
     3421 gttgcacgca ctcccaccgc cgacaagtcg cggcggccgc tgcccgaaag caacgacatg
     3481 ggattatagc gtattcgggc cgattgccga cccatggttg cccagcgtta aggtgacgcg
     3541 atagatgtcc tcgatttggg cgacgtgatc cgcctctgtg cgaacgggcg agatctgtgc
     3601 ccttaggtcg gccagccgtt cgggacgatc gatcagcccg cgcaggatct ctcgcagggc
     3661 ggccgcgtcg ccgggtggga acaatagccc gtcaacgccg tcgcgaacca tgctgcctgg
     3721 cgcgcccaga ttggaagcga tgaccggcag cccggcggcg aaggcttcgc ggatagtcgc
     3781 cggggaagcc tcataccaga gcgtcggcat caccagcgcg tcgagatcgc ccaaagtctc
     3841 ccaaagtcga tcccgcgcga gcaggccgtt gaactgaaca ccagggtgcc gattcatcgc
     3901 tcttgcctcg gccacatagt cggggaagac agccggatcg ccatagatat ccaggctgat
     3961 ggaatccggc aagccgttca gcgcttcgat cagcacgtgc agcccttttt gcgggctgag
     4021 gctgcccaga tagcccaatc ttatattgcc cgtcggcctc gtctctcgtt gggcggccag
     4081 gtgttggatc aattccggcg gcgtatcaag gccaagcggg ttgatgacga catgatcggt
     4141 cgagaaaccc attgtcgcgt ggatgttgcg gacgaactca ttcggcgcca gcacgcggcc
     4201 cgccccgacg aagatcggcc gcagcagcgc atcacggcgc cgcaaaacgg gtgccgcgac
     4261 cggcccgagc aacccggcga cgggcacaag tccggcgcgg gccacggcgc accggccgca
     4321 attatgaaac cggctatccg gcccgtcaca caacctcttc gtgtcgttgg tcagcagttg
     4381 accgttggca cagccatacc agtagtcatg gagcgatata acgtagggcg cgccggattg
     4441 tcggatggcg tcaacgatag cagccggtat gcccatcaaa tgctgcaaat gaacgacatc
     4501 gggctgctcg cgcgccatga cggcatcgaa tgcagcctgt aatccggcat ggccgaatgt
     4561 gctgcgaaag acggccgtgg ggctgcgccg gccggccggc acgcgataca ctcgcacccc
     4621 ttcctccacg gccggctgag cggagaaatg actatcccga ttcaggggcg tgaagacggc
     4681 tacctcatgc ccggcttttg cctggcggcg ggccacggac tgcgtataca gctctgtccc
     4741 ggctacgtga tcgggcacat actggtggac gacgtggaga atgcgcatga acgcctcaac
     4801 ccgtcccggt aatgcgccga tactgttcga gcgctcgcgc ggcgacgacg tcccaactgt
     4861 aatgggtacg gattttctcg cgtccggctt cgccgacgcg ctcggcaaaa gccccgtcgg
     4921 tgatcagccg ctcaacagcg ttggccaggc catccacgtc ggcaaacggc accagcagac
     4981 cattggcgcc gtcatcgaca acgccgggga ttccacccgc ccgcgcggcg acaaccggca
     5041 cgccatggga ccaggcttcg agcaggacga tgccgaacga atcggctcgc gagggcaaca
     5101 tcaggaattg ggcgcggctc agcaccgcgt gcttgtcccg gtcggaaagg atacccaacg
     5161 gccggatagc ggcccggtct tcgggcgaga gtcgccgccg atacttctca aactcaggcg
     5221 cggccgaacc gatcagcaac agggcaatat cgcgtccccg ctgccggaga aggcgaacgg
     5281 cgtcggcggc atggagcgcg cccttgtcga acgactgacg gccgatatag actccgaagt
     5341 cgccacgggc ctcatgatgt tccagcgaaa agtaaggcga ctcggtgtag tcatcgggaa
     5401 tcgggtccac gccgctgccg atgacgtcca gccgttcggg gcggacgtga tagcgggcca
     5461 acccatccgc ttccaggcgc gtgagcacca acacacgggc cgcgtcgcgc aacaggcgta
     5521 actggtggtt catcgtcgag ttacgagcta cgcgccccag gctcgactcg cccagatggg
     5581 caaaaggcgt ggccaccagc ggcacggccg cgcgccggct atactcatga gcggctacaa
     5641 ggccatgctc ccatgagatg ttgaagccgt gaacgacatc aaccccgcgc aggccatcca
     5701 gcgtctcgtc cagcccgtcg atggccggga aacggcgggc catgccggcc aacatcgatg
     5761 actgatcacc gggcatggcc gacaggagaa ccatgaggcg acggcggcgc atcagcgcat
     5821 cacgtccacc agccgcaggc aatatcggta agcgaatgac tcgcacgcca tcctcatcgc
     5881 cgtccgagcg tgattcaacc ccattccaga aatcagcttc ggccgtggca gagctggtga
     5941 cgacagtcac cttcgtgcct gcggcaacca aagcacgcgc cagcgccccg acatatcgct
     6001 cgccaccgcc cggcaatggc ggataggcgg gtgagaccaa cgccacatga agaggctggt
     6061 cattcatcag ggagagaccc ccggcagcag gtttagttgt tgcaaatacc caatgaattg
     6121 ttcacgcact cgcggctcca gaaaggcctg aacgcgcgag aactggcgat caatgacgcg
     6181 acgacgtaac ccttcgtcct ccaaaaacag gtcgacgagt tccgccagcg cctcgtagtc
     6241 cttgcgatgg aacaggacac ccgcgccgcc caacgtgtcg gggacggcgc tggacgcata
     6301 cgccaggaca ggcaagccac aatacatgct ctcgatcagc ggcttgccga acccctcgtg
     6361 ttcgctcatg gaaacgtaga gatcggccgc gcgatagtaa gtgatcatgt cctgatggga
     6421 aacgtggccg gcaaacgcca cggccgcatc gacgcccaga tcgcgggcca gcgcgcgctg
     6481 ccagtccaca tattcccggt tgtcggtgtt gccgaccagc accaggcgag cgccgggccg
     6541 ggcgcgacgc agatagtaga gcagcttgat gagatcttcc tgacgcttgt tgggcataaa
     6601 acgaccgaca aaaagcagga gcggtcggcc gttatcgcat ctcctgacta attcatcatt
     6661 cgacgaaatc gaatatgacg actcatcgag gacaatcggc aacacaccgg tcggcgtata
     6721 tcccagcgag attagctcgc cctcgctgta aattgaggcg cccagaccta acaggaccag
     6781 cggccgcatc tgcgccagtt gctgccgccc tcttttcagg ctgtcagcca atgccggatt
     6841 cgtctgctca aaaaattcag gcggggtgat attgtggtag atcaggattt gcggcaggcc
     6901 gatcttcatg agccgcccgg cgatgataga gccgacggcg tgatggtgga tgaggcatgt
     6961 ctcgaccgac cgcggacgat actcgccggc gggcagcacg cgccccgcca gttccggctg
     7021 gatgcgctcg gcgtatagct ccgacacaaa gcccagctcc cgcaaccagc gctggatgag
     7081 aaacgcctga tcgctgacgg cgtcgccgac gtaggctccc tcagtaaact gatgcaacac
     7141 ccgctcaggc atcatcgcct cggtgcggca tgtccgtcag cgtttccagc acggccctgt
     7201 atttcgccag aatgacgtcc caactgtagt tggcggtgac gaaggcgcgc ccctgacggc
     7261 cgagttgcgc gcgcaagtcg tcgtgggcca gcagcatcgt cagcgccgag gcgaactcct
     7321 cgtacgaata gtagtagagg ccgccattgc tctgccggga ctgatattcc agcacctcgc
     7381 agcggccgtt gagcagcgtc ggcgtcccca tcagccacgc ctccatcgcc acaatcgaca
     7441 ggctctcgaa gagcgagggc atcaccagca cgtcggcggc actgatggcg tcgaacttgt
     7501 cttcctcgga cacgaacccc agcggcacga tatccggccg gtcgggcacg tagaccttga
     7561 ccttgccaat cagcagcagc ttcagatcgg cgcccgtctc ctcgcgaaaa cggatgaagt
     7621 gatcgaacaa ctcggggaca ttcttcgact cctcgacgcg accgacgtag aggatgaacc
     7681 ggccgtcgat gccatacttc ccccgaaaac gggcggccga gacatccgcg gggacatcaa
     7741 cccccacccc ggcaacgatt tgcggtacat aatgattgtg catcacccga ttcaccagca
     7801 gcttctccgg caccgtgttg tagatgatca tctgcggcag gtgaaataaa gaccggatga
     7861 tcggcaagtg cagatagggt tcgtcgtggg cgttgggaat gaggactgcc ttgtcggaca
     7921 caagcggcag cccgaagaac accggcgggt agagataggt gacgaagata tagagatcgt
     7981 actcgtcgta ggaatcgtaa acgtaattga gcaggtccga ggagtacggc ccttgctcct
     8041 tcatccagtg gacctgggtg aagatggagc ggtggccgcc gaacaactcg ttgtgatgga
     8101 gatccgtgcg caggccgtcg acccgaaaac gatgaatcac cacaccgttc aactcgctct
     8161 cgcccggcga gtagacatcg gcccacgtgt gatgatcgac ggcgcaggtc gtgatcacgt
     8221 gcatctcggc cagcgccgtc agatgctcgg ccacccagcg agccaacatc tccgccccgc
     8281 cgttgacttc ctcgccgtag cgctgcacga ccatggccac ccgcttcttg ccgctcatgc
     8341 ctcgatttgc tccagatagc taaagaattg ccggcgcacc tgcggctcca gaaaatgctc
     8401 aacgcgtgcc cgctgcttcc cgatgatccg ccgtcgcaag gttgcatcct catagagcat
     8461 ggcgatcagt tcagccagcg gctcgaactc cttgcggtag aagacgacgc ccgcgtcgcc
     8521 catcgtctcc gggacggccg tggaaccata ggccatgacc ggcaggccca gcagcatgct
     8581 ctcaatcaga ggcacgccaa agccctcgtg gtcgctcatc gacatgtaga gatcggccga
     8641 gcggtaatgc gcaagcagcg cctcatgaga caccttgccc gtcaactcca gcccttctgc
     8701 cacgttcagg tcggccgcca attgttccac ccagcgatca taacccatct cccagcgatc
     8761 gccgacgagg gtcaggtggg cctggggatg aatacgccgc aggaaaaaga gcagcttcac
     8821 caggtcttcc tggcgcttgt tcggcgcgag acggccgacg aacagcaatc gcggctctgc
     8881 gccggccggt gtctccggca acgtgccatt caccgccgct tcgtactgtt cagcctgcaa
     8941 acaaataggc aataccgccg tgcgaataaa cccgttcagc agcaggtccc gctcgttgta
     9001 atgggaatcg gccagcgcca gttcggtcag gggttgcagc atcttcaact gggcaatgcc
     9061 ctgtcgcgcg agataagcac gcaggggatc gctgttctca aagaaccccg gcggggtgat
     9121 attgtgatag atcaggatga gccgcagatc gcgcccggcg agaaattcgg gcacgtcgga
     9181 gccgatgctg tgatgataaa tacctcgcgt ctcgccacgc gcccgacggt aggtgctcat
     9241 cggccgcgcc gccgtcgcca tgctgtcgtg aagatgccag gcgtagattt ccgactcgta
     9301 gcccgcctcg cgcagccagc gctggatgag caaggcttgt ccggtgatgg catcgccggc
     9361 ggttgcgccg gtgagaaatt gatggagagg gccgcgattc atgattgcca atgcgacagg
     9421 gccgatcagt tctcgcggcg ccagatttcg gcccgcaggg cgtcaatctc ggcttgctgg
     9481 tcctgaatga cgcgcgtcag ttcattgagc gtgctgacca gattggcatt ggttcggctc
     9541 tgctcccgta ccgacttatt gacataaaac aagaccagat tgtgcatttc gccacgaatc
     9601 ttctgccaca ggccgccgac caccggcgcg cgcgtggccg gcgaaggggc gagaagcggc
     9661 tcaaccgaag ccggcggcat cctgttcgcc tgctccagat agtagaacag gtgcgggctg
     9721 actcgcttct ccagcggcac gtcgggatag gaagagacgt agccgaaggc gggaaacact
     9781 acttccacgg cgcccaattc agcgcgacgg cgggcaaccc gttcctcgac ctggcgaaca
     9841 agttcttccg agctgacctg ctcatcatgg ataacgatcc acggcgaatc ggccgcgtgt
     9901 tgtgattcca ttgcgctcat ttcgtttccc ggccggccgg acgagtgttt agaaacgatt
     9961 cgatcaaacg gtctcgggta acgcgctcgg tcgccaacag gttcgtccag taatccagat
    10021 cggccgcttc gggaccgtcg ccaaagaaca ggcggtagcc ggcgcgaaca tcatccagcg
    10081 tcacgggcgc atcctggttg acgctcgcca tctctcccaa cgcaggcgtc tcggcgggat
    10141 cggcctgccc caaaatactg agcgcggcca gaatatgatg gttgaccttg gcctgattca
    10201 tcgccgagcg attgatgtag aacaccacca gctcatggaa tttgcgccgc acgaaatcca
    10261 cgaccgggcc gaccagcggc acccgtgtgg gcgtgacgag caaatcgact tcgtttcggc
    10321 tctgagccag ggcggcgcga aacagctctt cgtagtattc cggccgttgg ctgccggcgg
    10381 ccgcttccgg caagcgcacc tgcccgggca gcttgcgctc caggatttcg cggcgcacct
    10441 ccatcatgac gtgctcaata ttgaccggct cttctgtccg ttggccggag ctattttgat
    10501 tcattcgtga ccggttccag atagttcagc gtcagcaccg agacttcggt atcgacgcgg
    10561 taaggtttat gctgataact gctctccagc cggcggcgcg atagcgtcac ctgctccgcg
    10621 cggtaatcca gaccttgctc atccagccag ttggccgggc cgacccagat caccgttcgg
    10681 tcgttatttt gccacgtttt gatggtctca accagcacag gagagatcgt gtcgggtgac
    10741 tgccgcaggg caaagacccc gaaatcatag atgaaccgca gcggcgtgcc ccagatatcc
    10801 ccctcgccca ccggcgactg gtcattgaac agcaggacgg catcgggcgg caaaccggcg
    10861 gccagcgcgt cgatctgggt cgtcagaccg gcatgatcca cctgcgatac aaacccgcgc
    10921 gccgaccagc ccaaccccgc cagccagccc acagtgacgg ccgcgacgag caaaggcccc
    10981 gccgggtgtg gtaagcgtct caggaagccc ggcgctgccc tgtcccaatc cgcccggcga
    11041 aaacccaaat gacccaataa ataggctgcg ccgaggatga agaacggcat gacggccggg
    11101 acatagcggc gcatgacgta gacgtgatgg gggttggcgc tgatgttcca caggtacacg
    11161 cccgcaaaca gcatgcccag cgccagagcc atgacactcc gccgatttac gcgccacagc
    11221 atcagccctg ctcccagcac gcccagccag atgcccaacg gtgacagata ccagcccagc
    11281 cgaatccagt tctcatgatt ggtgactgga atctcgcccg cgctgaaggt gtcgggtcgc
    11341 aatgtagtta tgttaagttc aggacgaagg aaccacccgt agagcgcata ggcgaacaac
    11401 agggtcaaca gcacgtagcg gacgatgcgc tggacgtgat ccggcgcgcg atagcgccca
    11461 acgcggcgcg gcaaccacag aagcagcagc gccaacgcca acagtccgcc cagcaagaac
    11521 ggccacaggc ggcccagcag gatgaagcca tagccgaccg tctcgtagaa ataaggcgcg
    11581 ctgaggaaat ggccgtggag cagggaatgg atcaccaaca cgccaaaggg ggcggcgaac
    11641 cagctatcga gccgctgcca gccgcgcagc caacgccaga gcagcacgat gccaaacaca
    11701 ggcagaagca ccaggctatc gatgcgcacc agaaagacgg cgccgaacgc gcagcccgat
    11761 acgaaagccc acaacgcggc tggccggcgc tcttccaacc agcgcccggc gctccagata
    11821 ccggcccaca acaggaactg ggtcaacgcc tcgctggtgg gataacggcc gaaccagact
    11881 tgcaaccccg aaagcgacaa cccggccaac gccagcgcgc cggtcaccgg cccaccgata
    11941 tcccgtgcag tcagatagac ggccagcgac cccagcatga tccacagccc ggtgagcaat
    12001 agttccgcgc gcactcctga cgcccccccg ccggcaaagc cgtagacaat cgcctgcaac
    12061 accggatgca agggatagaa ctgcggcgtc aactgcccgg cggcctcgtt ggtcacatag
    12121 aaaccgggca gatagtacga atcggcgaag ggtgtgttgg gcagcggccg cagcacggcc
    12181 gcgcgcatcc ccggcgtcat cccggccagc agctcatcag tcacgcgaaa gccgccttct
    12241 tgggcaattt cagctcccag gcttatgtaa actccggcgt ccgcggcgcc aaagacgtat
    12301 tcatgcggcc gaaaatagag ccacaacgcc gccgccagcc atacggccag catcgcccat
    12361 tcccaacggg ccaatctatc ttcgggcgat gcgtattcgc ccgccgcatc ccccttcccg
    12421 gccccgcgcc atcgccaaaa accgagaagc ccgaccggca cagccaacgc tacgagcaag
    12481 gccggcaggc tgaagacccc caactctgcc agaatcagcg ccacccagcc gacagagata
    12541 actcccagcg tcagcgcagc aaatgccgtt ggcaggttcc agcgcgaccc gcgcgggatg
    12601 aaccgggccg caagcaggac gccggttagc gccgccagga tgagcaagag caacaccccc
    12661 aaccagctaa ccattcggcc accccaacac atcccggatg aattcggcgt aggccgcggc
    12721 cacgcttgac ggcgcgcaat gctccaccac atagtcgtga cccgcctgcc ccatcatcag
    12781 tcgtctttca ggcgacgtag ccagtgcttc catcgcctgc cggagcgctt cgtcattgtc
    12841 cggcggaacc ttgatggcgg ccgcgtccgg tatctcgctg taccagccat ggtcatagac
    12901 gatcagcggc cgtgccgccg ccatggcccg caaggcgacg gctgatgtct cgccaacggt
    12961 tggctgccgc agattcacga cgacatcggc ggtgcgaatc cagtccgtaa actcatccag
    13021 gttcctgacg tagccgatgc gattcaccgc atgtcccacg cccagccgca ccacaagact
    13081 atccagatca acgtccggcc gcggatcgcc aaccagcaga tagtgcgcgt cgagatagcg
    13141 ctcaagcacg tcgcgaaatg cgcgcagcgc gacatcgatc cgcttctcgg tcgtgatctg
    13201 accgaagctg ccgaagagga tcgcatgatc cggcaattcc agtcgatcac gcatcgagtg
    13261 actcgaacgt ggttcgacca acgccggaac taccgccaac ggcagatcgg ggcgatagtg
    13321 acgcgcccgt tcagccgcat agcggctgtg gacgatcaag cccagggatg cgtcaatgag
    13381 ccgctggttg agcggctctt cgaatagtgg cgagacggtg ctgccacggc gcacggcacg
    13441 ggccagttcg cggccgtccc caccgatcgc gtaggtcaac tcccggctat atgcagtcca
    13501 ttcgccacgc cccacggtgc ggtggcggat gaaatggtgg agaaaatagt cgtggagcac
    13561 caccgcgccc ggatatcgca gcagcatggc ataaacccac tcatgctggt cgctattgcc
    13621 catctgatag agcggcaggt caaactcatc atgattcacc acaaagtcgc tcactggtcg
    13681 aacggtgaga cccggaaggg tgatcgtcgc cggatcagga gtgaatacag tcagatcaac
    13741 caattcggcc agatggggca acaattcagc gctgtagtcg gcgatgccgc tgcgcgacgg
    13801 gggaagcggg ctaaaccagg caattcgcgg ccgccggtta atcttgtcct cgctcaaccg
    13861 ccgggtcgcc atcgacaggc gcgccgcgtc tcgcgtcaac gtcgtttgcg tccggtcgct
    13921 tcgcgcgaac aggtcgtcta cctcggcctg ccccggcggc cgtcccaacc gttcggccaa
    13981 cgcccgattg aaggccgatt gctgctccat caccggccgc gcctgccatt tcgtcgccac
    14041 gccgttccag gcccggcgta atcgtgcgat caagccgcca acgacgggcg cttgcgagtt
    14101 aaactcatac tcctgaagcg tcgcacgttg ccgaaggata ttcgacgggt cgggctgagg
    14161 gcggttgtcg gtcattgaac gggatgcgtc agtcatgcca cgtgatctcg ggcacggccg
    14221 cggggacggc ataggggccg gcggcgaagt cgtcgaagta gccgttggcc tgcaacgcgc
    14281 cataggaata gagcgcgtgg ccggccgtgc ccaggtcacg ggccgcctcg atgcgctgga
    14341 cgacatcgtc aaatgtgagt aaccccggcc caatgccgcc gatgccgggg atgataaaac
    14401 gaccgccgcg attagcctga tggtcggcgg ccaggattcg ccacctgtcc agcggaaacg
    14461 ggttctccgc gggcggcttg taggagtaga tcatcggagc cagcgcgtcg atatagccgt
    14521 tctgcaccca ggcgcgggaa tcctggtaat agtcactgta cccctggctg tagccccagc
    14581 cccatttatc ccgataggtg gcccagacgg ccgcgctcag ccatatcttt cgcccggcgg
    14641 gcacgacaac gtcctgataa atctcgcgca ccagcgtgtt cacctgctgc cgctgccatt
    14701 cgtcatatgg caagccttta ttcgtgaagc atggcgcccc gtacatcgct tcgctgaccg
    14761 gatcgcatga ggtggtgcgc ccggcgtaac gcacgcgatc cagatggatg ccgtcgatgt
    14821 cataacggtt caccagatcg gcggccaccg ctttcacatg ggcgcgaacg accggcgagg
    14881 cgggggtggc atagaggtaa tcgccacacg acacctgatg ctgcgtcgtc cattgcacac
    14941 cgttgagctt gccgtcggtc gtgccgtggg caccctgcaa ttggaagtag aatggcgtag
    15001 gagcgaccat tgccggaggg ttggtgcaat tgtcccacac cggatagacg ttgatatagg
    15061 catggagttg cagcccacgg gcatgagcgc gctgcacgaa gtaggccagt ggatcccaaa
    15121 gaggcgcagg ggcctgacca tacgcgccgc cagtcacccg ctgcgcccat ggttccagac
    15181 cgggcgcgta gtaggcatcg gccgccccgc gcacctgaaa caggatggcg ttgaacccgg
    15241 catcagcggc gttgttgacg atctcatcga tcttggccgg gctggccggg gctttcgagc
    15301 tggtccagtc gaatcgcgtc acccacaagc cgcggaactc gaccatgccc ggcggcggcg
    15361 tgggtaaaac agcgatctgg taaatttcga gaaaggccag tttgttgttg acggcgttgg
    15421 gaccgctgcg aaccgtgagg cgaccgtcgg tcacggggac ggttccggct acgccatgga
    15481 gaaaacggct atcaaccgat ggcgcgccgt ccgtcaatgt gcgcccttcg gccgtgctgt
    15541 tcatatagcc gtcccaggct tcgggatcgc cggccacggc atagatatca tagatgcccg
    15601 gcagcagagc gatctcccaa accgcgtcgg gattctccgg cttctgaaga tggatgaggg
    15661 tgtcatagcg ctggtcgggc gcattggccg agttgcgctc gcgcgtggcg gcgctattat
    15721 cggcgttcca accgtagcgg aaaccgttgc cgcgatcggc gtaggtcgcg ccactatcgg
    15781 ccagatagcc gggcggtacc gccgcgccgg cgggctggaa gttgatcttg acgattggat
    15841 tgccgttcga cgctgcgtca cgctgcacga ccggcaggaa agcgcggctt cctccctggg
    15901 ccagcgccgc caaagtcagg cccaacaggg ccagcagcag cgtcattgtc gacgcgatcc
    15961 gttggtttcg attcatcatc tccttgttat tttgccacag atagcgcgtt cgtcccatat
    16021 tggcgattgg cgtctcatca agacctgaca ggtcggcgat gcgacacctg tctatctaaa
    16081 cccctaatcg ccgacctgtc aggtctgccg ccgctattgc tccaattgac acctcatcaa
    16141 gcggtctcta tactcggcta atctaacatg attctcccga ggcgagcttg cgatgagcat
    16201 acgattccat gacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatcta
    16321 acatgattct cccgaggcga gcttgcgatg agcatacgat tccatgacat cacccgaacg
    16381 atgtttgccg gcatgtccgt gtggccgggc gacgcgccgt tcgagctgga gccgaccacc
    16441 ggcatcgccg aaggctccac agtgaacctg acccgcctga gcatgagcgc ccataccggc
    16501 acccacgtcg acgcgcctta tcacttcacc gacgacggcg cgacgatgga acaagtcgac
    16561 ccggccatct actggggatc ggcgcaggtt gtcaccgtca aaaaggctac tggcccgctc
    16621 gtcccggccg acttcgccgg ctacgatctg ggcctcgcgc cgcgcctgct ggttcattca
    16681 ggcgccagcg cggccgaccc gcgcatattc ccgcaggagt tcgcctaccc cagcccgaaa
    16741 ttggccgact atctgggcgc gctcggcatc atcctctacg gtaccgacgc gccgtcggtc
    16801 gacgcctcgg acagtcagac cctggatggc catcacgccc tgcgtcgtaa caacatcgcg
    16861 atcctcgaag gtctcgacct gaccgacgtg cctgatggta tttacgaatt ggcggccatc
    16921 cccctcaaaa ttctgggcgg cgacggcagc ccggtgcgag ccatattacg gactacagga
    16981 gcaaacgaat gacatctcca gactttctca gccgcgaaca tgcccgcgcg ctcgacgcgg
    17041 ccgatccgtt gcccgccttc cgggagcggt ttgtcatcga tgatcctgaa ctcatttatc
    17101 tggacgggaa ttccctgggt cgcctgcccc gcgccacgat tgacctgctg gacgatctcg
    17161 ttcgccggca atggggcggt cgcctggtgc gcggctggag cgagggctgg ttcgacaccg
    17221 ccaaccgcat cggcgacaag atcgccggta tattgggcgc aagcgaaggc gaaatcatcg
    17281 tcgccgactc cacctcgaca acgctcttca agctgggaat ggcggcgctg atggcccgcc
    17341 ccggccgccg gaccatcctg accgacgacc tcaacttccc ctccgacctc tacatcctgc
    17401 agggcatggc ccggctcgcg ggcgacgact atcgggttcg ggtgatccct tcggccgacg
    17461 gcatcaacgg cccggccgaa gcattggccg cggccatgga cgaggacacg gcgctcgtca
    17521 ccctctccca caccgtcttc aaaagcggtt atcaatatga tatggccgcc atcaccgccg
    17581 cggctcataa tgtcggcgcg ctggcgctgt gggatctgag ccactcggcc ggttccgtgc
    17641 tggtcgacct gaacggcgcg gcggccgatc tggccgtcgg ctgtacgtat aaatatctca
    17701 acggtggccc cggcgcgccc gccttcctct acgtgcgcca cgatctgcag gacacgttgg
    17761 caaaccccat cagcggctgg atcggtcagc acgacccgtt cgatttcgcg ctcgattacg
    17821 tcccggcggc gggcatccaa cgcttcctga ccggcacacc gccctacctg tcgctggcgg
    17881 ccatcgagcc gggggtcgat ctgctcctcg aagcggggat ggatcggctt cgcgagcgct
    17941 ccgtgcggct cagcgaatat ttcatcgcgc tgtgggaagc ccggctggct ccgctggggt
    18001 tccggctcaa ttccccgcgc gaagtagcgc ggcgcggatc tcacatctcg ctgggccatg
    18061 atgaaggcct gcgcatcaat ctgtcgctca ttcgcgacgt gaaggtcttg cccgacttcc
    18121 gccgccccga taacatccgc atcgggatcg tcccgcttta caatacattt gccgagttgc
    18181 acgaggcggt ggatcgcctc gtgacggtgg tcaacgatcg cctctacgaa cgccacgaca
    18241 caacaggtct aaaagtaacc tgaccggcaa cgatccccgg aattcattgt catgcaccca
    18301 ccactacgct ttgtgaccgg agatggccgg atcatctact cgttccgtgt ccggtcattc
    18361 cctacgctgg tcaacaatat ctatctcatc gatggcggcg aggggctgat tctggtcgat
    18421 tgcggatcgg gcatgcccca ggccaatgct gagctggagg ccgggttcgc ggccgtcagc
    18481 gagacgttcg gcctggctgt gtccatggcc gacatcagcg ccatcctgat cacccacggc
    18541 cacatcgacc acttcggcgg cctgaatttc gtccggcagt ttaccgacgc ccccatcggc
    18601 gtccatatcc tcgaccggcg ggtgctctcc aaccacgagg agcgcgtcgt cttttcctat
    18661 cggcgactgg agacctttct ggagagcgcg ggcgtctcgg ccgaactgcg cgaggcgttg
    18721 atgagcgtct atctcttcgc caagacctac taccgctcga cgccggtgca gttcctgctg
    18781 gaggaaggca agcccacaat cgacaacatc ggcgtctacc acgtgccggg ccattgcccg
    18841 gggcaggtct gcctggctgt ggacgacatc ttgctgacgg ccgaccacgt cctgtcgcgc
    18901 attacccccc atcaggcccc ggagagcatc acccaccaca tggggctgag ccattatctg
    18961 gattcactgg acaagatcgc cgcgctatcg aactatcgcc tcggcctggg aggacatgaa
    19021 gacccaatcg acgacatccc ggcacgcatt ggcgccattc ggcaggccca tgatgagcgg
    19081 ctgagcaagg tcatggcgat ctgccgcgag ccgaaatcca tcgccgacat cagccgcgag
    19141 ctattcggcc gcgtcgattc ctaccacgtg cttctggcgc tggaagaggc gggcgcccac
    19201 gtcgaatatc ttcaccagcg cggggaattg atggccgcca atctcgaaga gatcgaacgc
    19261 accagtcacc cggtcgtcca gtacgtgcgg ggctaacggc cggcacccat gagttctcac
    19321 cccgtcgccg tcgccctgct cgcctggtgg gacgccggcc acgccgacct gccgtggcgg
    19381 cgcacccatg acgcctatgg ggtgtgggtg gccgagatca tgctccagca gacgcagatc
    19441 gccaccgtca tcccctacta cgagcgctgg atggcgcggt tcccgacggc cgaggcgctc
    19501 gccgccgcgc cgctggatga tgtgctcaag ctgtgggagg gtctgggcta ttacagccgg
    19561 gcgcgcaatc tccacgccgc ggcccaaatc gtgacccatg agcatggcgg gcgcttgccc
    19621 agcaccgtac ccgaactgat gaagctgccg ggcatcggcc gctataccgc cggagccatc
    19681 gcctcaatcg ccttcgacgt ccccgcgccg atcctcgacg gcaacgtcat ccgcgtcctc
    19741 agccgcgtcc ataacatcga agaagacgtc acccaatcgg cgacgcgcac ccggctctgg
    19801 catctggccg aggaattagt gccactcaaa cgcccgggag atttcaatca ggcgttgatg
    19861 gagctagggc aatcgatctg cctgcctaaa aacccgcgct gcctcatctg cccattgaac
    19921 actctgtgcc tggcgcggca acacggcacc caaccggagc gtccggtgcg gccaccgcgc
    19981 aagcgaacgc cccactatga tgtcgtagcc gccatcatct ggcgtgaggg gccgccgggg
    20041 cgtcatgcca agttcctgat cgcccaacgg ccgcatgacg ggttactcgg tggcttgtgg
    20101 gagttccccg gtggcaaggt ggaagcgggc gagacaatgg cggatgcgct tcggcgggag
    20161 attcgcgaag agatggccat agacatcacc cccggcgcat tcctgatcgc ggttccccac
    20221 gcctacaccc attttcgcat cacgctccac gccttccatg ttatctacgg cggcggcgag
    20281 atcgaacatc ttggcgtagc cgatcatgcg tgggtgacgc tcgacgacct ggaccgcttc
    20341 gccttcgcgg tgaccgaccg caagatcatc gaacagctgc gccggacgct aatgccggaa
    20401 cagccgcaat cgcaaaccgg agcggtttag ataagtgatc gtttcccgaa aacgggccgg
    20461 ctggaaaccg aactggcgct gaacgacgtc cagatcggtt acgtcgggca caaagaaccg
    20521 gtcgacaaag taacggctga tccccggcca ataccaccag cccagcagga tgcgcgagac
    20581 cggccgcatc agggtcatcg gcagcggcag gagcagtcgc tgcttgccgg taacgttcaa
    20641 tatcatccgc acgaattcgc gatagctgac caactcattt ccggcaacga ccggcgtccg
    20701 atcgatcata tcagggcggt cgagagttcg aatcaggcag tggacgtaat cttccaccca
    20761 cagcggctgc atctgcatct tgcccccacc aggcagccac gccagcggcc acgtccatag
    20821 tgccagacta taaacgatct cgctatagcg gtcatcccga ccaaacaggg ttgaggtgcg
    20881 caaaatcgta taggggatgc cgctctgccg cacgagccgc tccgcctccc ccttcgcccg
    20941 cagcagaggg tgcatcgaat aggggttggc gttcaggcgg ctgggataga tcaggcgcgt
    21001 gacccccgcg cgccgggcct cctcgatcag ccgttgcgtg ccttcgatat cgacacgctg
    21061 cagcaggcga tcgctgccgc gagcttcggc tccggccagg tggatgatcg tgtccgctcc
    21121 ctcgatctcg gcccgcaaag cctcaggcga gttcatccgg cccccatagg cccgccaggc
    21181 atggccggtg tggtcgagcg cccgacaaag cacgcggccg atgaatccgg tggcgccggt
    21241 gatgaggatc atgggcgcaa ttgtactgcg acaacggctc actgctataa tcgcaaacaa
    21301 tgagccgctt ttcgcctgaa aatcccttgc gcgtgtgcta cttcggcacc tttcgcgcag
    21361 gttatacgcg caaccagatc atcctcaagg gcttgcaggc ccaacccgac gtcgcggtac
    21421 acgtttgcca cgccacgctg tggcagggca tcgaagaccg cgtggcacag gccagcggcg
    21481 gctggcgcag ccccgccttt tggcgacggg tggccggcgc ctatcgccaa ttgctgcgcg
    21541 agcaccggca agcgcccgaa tacgacgtga tgctcatcgg ctatcccggc cagttcgacg
    21601 ccttcctcgg ccgccggctg gctcatcatc gtggcaagcc ggcagcgctc gatatcctga
    21661 tgtcgctgca cctggtggcc gaggagcgcg ggctaacggc cgcccacccc aacacgggca
    21721 ggctgatctt ccaactggag cgcagcggct tgcgccaacc cgacttgctc atcgccgaga
    21781 accccgaata tggcggctac atcgccggca aataccaact gcccatcgag cgctttcgct
    21841 acgtccccca cggcgcagac gagacggtgt tccatccccg accattacgg ccgccggaca
    21901 attacttccg cgtcacctac cacggcggct atctgccgtc ccacggcatg gacgccattc
    21961 tgggggcagc ccgcctgttg gccgatgaac cgggggtgcg gtttgagttc tacggcagcg
    22021 ggccggagaa ggagcgcatc gtgcgccagg cccaggaatg ggaactgccc aacaccacgt
    22081 tccacggctt cgtcagccag gacgaattgc tggatgggct ggcccaggcc catgtctgcc
    22141 tcggcgtttt cggcaccacg cggcaggcgc atttcactgt tcagaacaag gtgtgggaag
    22201 ggttggccat ggggcggccg gtcatttccg gggattcgcc caccattcgc gccgcgctgg
    22261 ctgaccgcga ggagatctat ctcatcgcgc gcagcgaccc ggcggcgctg gcagcggggc
    22321 tgcacgaact gcgcgacgat ccggccctgc gcgagcgaat cgccgcagcc gg
//
LOCUS       DAOMQG010000018        22030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1015_length_22030_cov_9.242776, whole genome shotgun sequence.
ACCESSION   DAOMQG010000018 DAOMQG010000000
VERSION     DAOMQG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22030
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1015_length_22030_cov_9.242776"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLC06_02295"
     CDS             <1..776
                     /locus_tag="PLC06_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO89304.1"
                     /translation="RYLVQGQDLIFNLAGQVSHTDSMADPYTDLEINARSQLSLLEAC
                     RHGNPTAKIIFASTRQIYGRPAYLPVDERHPLQPTDVNGINKLAGEWYHIVYHNVYGL
                     RTVSLRLTNTFGPRMRIRDARQTFIGWWIRQLLEGQTLNIFGDGLQVRDFNYVDDVIE
                     AMLMVAAHDVADGQIYNLGGDEPINLINLARLMIEVNGGGDFVLKPFPDDRKRIDIGD
                     FYGDYRKIRSKLGWRPMVGLRDGLTRTMDYFRANLEYYL"
     gene            782..1897
                     /locus_tag="PLC06_02300"
     CDS             782..1897
                     /locus_tag="PLC06_02300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRO89305.1"
                     /translation="MTTLENIPFVDLRTAYHRLRTEIDAAVADVLEGGWYILGEQVRA
                     FEEEFAAYLSRESVVSVASGTDAVLLALRACGVGPGDEVITVSHTAVATVAAIELCGA
                     IPRLVDIESETLTMDPQGFRAAITPRTKAVVPVHLYGCPARMDDILVIAREHGLVVVE
                     DCAQAHGARYGDRMVGTMGDAAAFSFYPTKNLGAMGDGGAVATSRADVAERLRLLRQY
                     GWKERYISDVPGYNSRLDELQAAILRVRLRHLDEENEARRRLAAAYDAHLAGLPVVRP
                     VPRPEDHHVYHLYVIQTERRDALRAFLQGRGIGTAVHYPVPVHRQPAYERLGYGLGSL
                     PVTERAAERLVSLPMYPDLSPDAPALVAAAIAEFFRQ"
     gene            1928..2365
                     /locus_tag="PLC06_02305"
     CDS             1928..2365
                     /locus_tag="PLC06_02305"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRO89306.1"
                     /translation="MTASLGTIRQFLVAHFSDEELDQFCFDHFYEAQQHFATGMSVNR
                     KAMVLVDYCHRRGLVPALLDELRKERPEPYRLAFGGRARPAIRRIDINTATADELLEL
                     PGIGPALAAAIIAARPFDRVDDLARVPGIGRKRLAAMREWCSA"
     gene            2543..3517
                     /gene="gmd"
                     /locus_tag="PLC06_02310"
     CDS             2543..3517
                     /gene="gmd"
                     /locus_tag="PLC06_02310"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO89307.1"
                     /translation="MPTALITGVTGQDGSYLAEFLLERGYRVVGMVRRTSTLNFHRIA
                     HIQDKLELVPGDLSDQISLIRILEEYQPDEVYNLAAQSFVQTSWNQPVFTGDVTGLGV
                     TRVLDAIRIVNPKIRMYQASSSEMFGKVVEVPQRETTPFYPRSPYGVAKVYGHWITIN
                     YRESYNMHATSGILFNHESPRRGLEFVTRKITHQAARIKLGLADELRLGNLDARRDWG
                     FAGDYVRAMWLMLQRDDPQDYVISTGDTHSVEQFLGVAFGHLDMDWREFVVQDERFMR
                     PAEVDLLVGDPGKAGRDLGWEPKVTFAELVRMMVDADMELIRNGEAAV"
     gene            3604..>4184
                     /locus_tag="PLC06_02315"
     CDS             3604..>4184
                     /locus_tag="PLC06_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRO89308.1"
                     /translation="MTKIIFGTDGWRGRIAEDYTFDNVRRCAQGFATFLHRQGIAERG
                     VVIGHDQRFQAEFFAAASAEVLAANGIKVWLTQSNTPTPAISYAVPVRNAAGAINITA
                     SHNPPHDCGFKVRDETGGAVPPDDLKRIESAIPDIDGVKRMKLDDAMSDGTVVKFDPA
                     PEYIALLNRLVDIEPIKAAGFDVLVDCMWGNGAG"
     assembly_gap    4185..4284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4285..4875
                     /locus_tag="PLC06_02320"
     CDS             <4285..4875
                     /locus_tag="PLC06_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRO89309.1"
                     /translation="YYFLEIRGERGPIVKTISTTKMLNKLGEIYNVPVYETGVGFKYI
                     APKMTEVGAMIGGEESGGYAFRGHAPDRDGILGGLFLLDLMVKTGKKPSQLIDWLFAK
                     VGPHYYDRIDTAFETSRRDEVRQRVLAAQPKTIAGLKVTEKSTLDGFQFFMEDGGWLL
                     IRFSGTEPIIRVYTETTHGDKVQDILQDGLRIAGLK"
     gene            4878..5363
                     /locus_tag="PLC06_02325"
     CDS             4878..5363
                     /locus_tag="PLC06_02325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRO89310.1"
                     /translation="MEKAFQDYYPDEVSYCYGCGRLNEHGLQIKSYWEGDEAVCHYQP
                     RDYHTAVPGYVYGGLIASLIDCHGTGTAAAAMYREQGRAMDTEPPLRFLTASLHVDYL
                     KPTPIDLPLELRARVKEIKGRKVIINIDLIAHGEVCARGEIVTVQVPDEFMARILGKD
                     G"
     gene            5363..6157
                     /locus_tag="PLC06_02330"
     CDS             5363..6157
                     /locus_tag="PLC06_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRO89311.1"
                     /translation="MSDSPVVLMDTHCHLDFVAPIDKVEEVVGRAHMAGVERILVPAI
                     EMDNLPRVLSLAEKFTGVFAAAGIHPNSAAGWRDEWVDRVREAAKHPKVVAIGEIGLD
                     YYWDKTPKETQHTALARQLELAAELSLPVIIHNREASADTISTLAASPLNGSDRPGVL
                     HSFSGDWATAEAALEMGFYLGFTGPLTYKKADDLRAIAARAPLDRILIETDAPYLTPE
                     PYRGKRNEPAYVRLVAERLAEVRGLSLPEIATATTANALRLFGIAH"
     gene            6252..7175
                     /locus_tag="PLC06_02335"
     CDS             6252..7175
                     /locus_tag="PLC06_02335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89312.1"
                     /translation="MLSVIIVNWNVRDLLRECLQSIEAGRDGLLLEIIVIDSASSDDS
                     AAMIRSEFPSVHLIACTENVGFPRGNNIGLREARGDYLLLLNPDTVVLGDALKVLVSY
                     LQANPDVGAVGPQLLNPDGSVQSSRRRFPTAATGFFESTWLEGLAPGVLRRYYALDLP
                     DDTTADVDWLTGACIMVPRSTWEAIGGMDEGYFMYSEELDWCRRIKESGRRVVYHPAA
                     QVIHHVGKSSEQAATARHINFNRAKLRYFRKYHGRFPAAVLRVFLLAGYAWQMALEGF
                     KGLLGSKPDLRRQRIRAYSEVLKSGLRPAGY"
     gene            7179..>8110
                     /locus_tag="PLC06_02340"
     CDS             7179..>8110
                     /locus_tag="PLC06_02340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89313.1"
                     /translation="MRVGLVTGEFPPMEGGVGAFTEQLGRALKVLGHEVHVITSIKAR
                     PKDAPRTLARLREPILLDFGYLHPRIGRWRWPSISAVADLVIRHDIELVNLQYQAAAF
                     NMNSAAISYLPWRLKGIVPTVTTFHDLRVPYLFPKAGRLREAAVRGLARRSAGCIATN
                     PADFRQLTAWSDRLVRDIPIGSNIATHEPNHIEIEEARLQLGLRGDDVLLGYFGFLNE
                     TKGADTLIDALARLDERFHLVFIGGQTGASDPDNNQTFLGGMRDRITQAGLANRVHWT
                     GFLSPERVSTYLTAADLMAMPYRDGVSLRRGTLMA"
     assembly_gap    8111..8210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8211..8542
                     /locus_tag="PLC06_02345"
     CDS             <8211..8542
                     /locus_tag="PLC06_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89314.1"
                     /translation="MAMPYRDGVSLRRGTLMAVLAHGRPLITTVPAEISPEFRHGENM
                     WLVPVGDVDALCGAIVTLTADPSLRESLGHEARALARSYRWDVIAGQTVSLYSEVLGS
                     WVGGSLK"
     gene            complement(8497..9018)
                     /locus_tag="PLC06_02350"
     CDS             complement(8497..9018)
                     /locus_tag="PLC06_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89315.1"
                     /translation="MKGSISIRPIMVADELFLWEALYLAIYTPPGKLPPPREIVHSPE
                     LSRYVAGWGQAGDIGFIAEENGQPIGTIWLRLFDGGRRGYGYVGDDIPELSMAVLPEN
                     RARGIGSGLLAHLLDVAACRFQAVSLSVSRNNPARRLYERYGFKKVGESADSLIMLRH
                     FSEPPTHEPRTSE"
     gene            9132..11492
                     /locus_tag="PLC06_02355"
     CDS             9132..11492
                     /locus_tag="PLC06_02355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89316.1"
                     /translation="MLSHPRAERRLLSLILALYLILGFTYALLTPPFEASDELWHYPM
                     IRHLADGNPLPVQVFDPALAGPWNQEASQPPLYYYLGALLTFPIDQSDMETVRRQNPH
                     VDTGVLTPDGNTNLVVHDPAADPWSGSLLALRVVRLFSVLLGAATVYLTWRIADETAP
                     GRPEIALGAAAINAFLPMFLFISGAVNNDNLALPLTSLALLLMIRIVTQRKESYHPVR
                     WWEWVSIGIVVGSAALTKEGTLGLFPMIFGTAIVAAWQQLVAGSRDSRGNLSAVIWPV
                     VRGLLSAAVFAIVPALIIAGWWYYRNIVLYGDWLGWSAFIAVLGERGHAASLIQLWGE
                     RRGFMMSFWGLFGGVNVSMPMWIYATLNAILIVSVAGFVFFFIRLLSTEWRGFRAARE
                     PWPARLLRPIERNFGLIVNLVFSAAIVYGLIQWATTTWSSQGRLVFTAISSLSTLMAL
                     GLAGSADLANKLTRGRISLRPWLIGGVAAFMLLVAAAAPWLWIRPAYQPPTVSGPLAE
                     RLDAEFGGVVRLNSYTIEHEGELRPGSEIRVRLEWESLQATERDWSVFVHLNDPVLGH
                     PIAQRDMYLGRGLLPTSFIVPGQQIVDEYVLTIPTTAVAPADLELVVGLYDFRTGERL
                     ATATGEETVSLGIIPLLPAEGAYPNPVSLFFEHGLELVGFSVEPRRVAAGESLELVAY
                     WRPSAPLPDDYTFFAQIVGPDTTRYAAADIAPPQPTSQWTTGKVVELRYPLTVDPAAP
                     PGAFPLILGLYTMTPDGGFDRLQQVTPDGRLTDDFLVLTTVRVDTP"
     gene            11489..13006
                     /locus_tag="PLC06_02360"
     CDS             11489..13006
                     /locus_tag="PLC06_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO89317.1"
                     /translation="MTTGHEPIAGERGRRGEGEKERPLTTSGGRRSVVGRQLGPADSA
                     LSLGLLIGATAVLAGIILAFGGPIAGAAVVIGGLATWLVLRNIELGFFAVIAVVAILP
                     FATLPVDIGLTPTFLDFALAAAIGVWLLGIVTGRQRRLITAPVALPLILFIIVAVFAF
                     IFGLANGPLTPTLLRKFAELLLSLGFVIVVIDYCRTWVRLERLVKFLLLMGAAAAVIA
                     IGLWLLPDATANSALTALGRVGYPGGWVIRYIEENPELSERAIGTSVDPNSLGGLLLM
                     IGALVGPQVVTRKPIFRRWIIWGITGLVFLALILTFSRGAMLGLAAGLGFVALARYRR
                     LIPYMLVAALLLLFLPVTQAYIARFLEGFQGDDLATQMRFGEYKDSLRLIARYPLFGV
                     GFAGSPDIDLYTAVASVYFTIAGRMGLFGLSAFFGVIGSVFAYAFRNRKAARIDERRD
                     AVWLGLHAALIGALVAGVFDHYLFNMDFHHAVTIFWMMLGMAVAATRLVREEGIS"
     gene            complement(13140..15662)
                     /locus_tag="PLC06_02365"
     CDS             complement(13140..15662)
                     /locus_tag="PLC06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRO89318.1"
                     /translation="MNSKNWERFTQRARRVLSLAQEEAERLNHSYIGSEHVLIGLLLE
                     EGGVAGRVLRELGLEVTRVQAMVERLSGGQGTRTPFSKTELSPSTKRILEYAVEEARR
                     MGQHYISTEHLLLGLARQNEGLAIDVLRKFGISAEQIRRQTRRMLKESPVSTTETPQP
                     QQPRRQQQQQAKKEKSKTPLVDQLATDLTALAQEYALDPVIGRQTEIERVIQILARRT
                     KNNPALIGQPGVGKTAIVEGLAQRIVDGQVPESLQNKRVLQLDVGSLVAGTMYRGQFE
                     ERLKRVIEELKSSDAILFIDEVHMLVGAGSAGSSVDAANILKPALARGELQCIGATTL
                     DEYRKYIESDAALERRFQPVRVDEPSAEETVEILKGIKGAYEQHHNLLISDEAIEAAV
                     FLSTRYVTERFLPDKAIDLIDEGASRVRMYRVQQPTDVREAYEALRDLRTQRTEAVEA
                     GRYEDATRIREQEEDIQEQLNELRAGQINGDDSPVLTAEDVAEVLSMWTGIPVYQLTE
                     EESARLLRMEEEMEKAIVGQKDAIVAISKAVRRARAGLKNPRRPIGSFMFLGPTGVGK
                     TELTKALATFLFGSEDALVQLDMSEFMERHNVARLVGSPPGYVGYEDAGQLTEAVRRR
                     PYSIVVFDEIEKAHPEAFNLLLQIMEEGHLSDAKGQKVNFRNAIIILTSNVGADMIKK
                     GGGLGFNFKQDASVLVEENYRDMQKKLMEQLKRSFRPEFLNRLDSVIVFRQLQPEDIR
                     QIVDIQIGDVNERMDEHFLMLEATDAAKDWLAIHGYDAEYGARPLRRLIQQTVEDKLS
                     DAVLAGDFQEGETVIIDVENDEIVLRHPTPTEEAGEELLPTI"
     gene            complement(15718..16833)
                     /locus_tag="PLC06_02370"
     CDS             complement(15718..16833)
                     /locus_tag="PLC06_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRO89319.1"
                     /translation="MNFLLNEEVYLPTAGEIRTGRIVDLRNNEILVDIGAKSEGLIPN
                     QETAGFDSATKELLKEGEEILVFVVNPEDNNGNIILSYRRAAEEADWQLATELLESND
                     AIETEIVGFNKGGVLVSVGQLRGFVPNSQMRRDRLQEIEGDNRQRSYQSLVGQPMLAK
                     VIEVDRPRNRLILSEQAASREIRQARKSELFSSLEEGAVFEGQVVNLADFGAFVDIGG
                     VEGLVHLSEMSWKRIQKPGDMVQKGDRVKVQILSVDQDRQRVALSMKRLESDPWTRIE
                     DLYRVGQLVEAEITRLTKFGAFARVNDDYQLEGLIHISELADDRVEQARDIVKPGDKI
                     TVRIIRLDSKQRQVGLSLRQVASDKFIEEDLAMLNTM"
     gene            16988..19945
                     /locus_tag="PLC06_02375"
     CDS             16988..19945
                     /locus_tag="PLC06_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89320.1"
                     /translation="MAGKAGFTVGARNAGAPTWLFRLFPFLLFSLSFLPRLVAIGRYV
                     TPDEPTWVYRSVQFREALLAGNWAGTLVAGHPGVITTWLGAIGASVHMLFSADVAEAY
                     SWLLKVAFLTPDNVAAFERLAMLLSSGRVAIALVNSFGVVAICLLARKLWGMKVAIVG
                     GLFLALDPFLLGLSGLLHVDGLSATFVTISLLSLAVGLRSGARDGSVNHKWMALAGFA
                     AALAALSKTPSLLLLPVSGVALLWGLVAGRDTPFGARLAAFLRWAIVWGASFAVTVLV
                     LFPAAWAAPAAVIGTLGGSANRHLDEALRQTFFMGEAAFVHGPLFYPIVLLWRLSPVV
                     WLSLPALGFLFIVRKRIKDAVSHNRPTVILLALWIILYLGMITMAAKKFDRYVLPVVP
                     SLLLLAAIVWVELSRVRPRAGRRLLACAVAIQGLFLLVHASYPLAAYNPLVGGSRTAL
                     GVLPVGWGEAIGAAGAYLDQAVPDASQSRAIAPIAPALAPFFSGQTLVEGYDDPATAD
                     YVIVTAEERQLDSIGVSALTDGMELQRTIRFAGIDQAWIYRNPLPLPPITPEELDAPA
                     TFGERIALLAFSQSVDDDRIDLHTRWQRLISTNEAGRYTLRIVIKDGADNLWASQETP
                     LLNETYFYPPDWPQAQTEMVRYQLELPPGVPPGEYQVVLSLIDDATAGRLPVRVGIDD
                     FHGVAYEAGRFDLTLPDAIVSASRMQIVVAEDATWLDGRLQLLGREEIAAEALAGSRL
                     PVHLFWHAPQGGLEAGLGLRWLLRPGDGSADIEVDTIALSRFDTGLWRTGESMQEIYQ
                     IPLPPDLPPGSYQLLLSPLTQDGDLAGDALSLAELQINNIDRLYELPSELPVDLDVRW
                     EPLTLEGMSPAEISTVAGEPVEVTLFWKKQHPHGDVYSVFIHVVDEAGNIVAQSDHWP
                     GGLPTNILDVGQVVVDKASIEIPATTTPGSYRMRVGLYSAESGLRLPVISSATGHSTD
                     YVTLPVALDVVSP"
     gene            complement(19965..20984)
                     /locus_tag="PLC06_02380"
     CDS             complement(19965..20984)
                     /locus_tag="PLC06_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89321.1"
                     /translation="MPKHVLMTVSLITALLIALASSQPRVIQAFDPSFPTRTPTPGPG
                     GATAEPTATSGDPGSPGNPGNPSPTGTSPSAPGSGSPQPSPSSAPGVPGTGSGLGTPI
                     PTVVLGGTIRVNPGGSGECSDTPYIRALGRLIIYGGPGVDYGPVSTLEVGEMRPITGR
                     AAYAEWWQILVKPNMIGWVQDREVEEFGNTALVPIAEPPAINGATPTPGIPWNPTPLP
                     LLTCVPTPTPTATPTATEMSVAPGGGEETGASAPESEAQQPDMVSAEIVATPVSQGQP
                     AAGGETGDSAAGPGMVSRGSSASRATSPTSVTNLLLPLVGLALIAVGIVLALASRKRG
                     TPKAE"
     gene            complement(21008..>22030)
                     /locus_tag="PLC06_02385"
     CDS             complement(21008..>22030)
                     /locus_tag="PLC06_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89322.1"
                     /translation="IAIDQEFLRVLVDATGPVPIPGTDQRISADNVLQTLRQARDIQE
                     GQEIQEWVRDRKAFLGGFAAAIQAKIETDFGSIDPLKLAANLAGAAEDRHLSIYMRDP
                     QLSAVLTANGWDGSLPQTPPGDFLMVVDTNMGYNKANIFIERALDYAITLAAQPQAHL
                     TITYHHTGERTGEPCYQGVDQEFEEAAEYLAIADQCYWSYLRVYAPAGSTLLDSSRHV
                     VPGETLYSGITWGSAGQMVEDIPWLTAFANFLLVPRGEEVTTTFTYQLPAGIIVPLAS
                     GESLYQLDIRKQPGMKDERLRIMVNLPDGATMLDASPTPVTIDGTQISFELNLKANEL
                     VAIRYR"
BASE COUNT         3813 a         6492 c         6704 g         4821 t
ORIGIN      
        1 tgcgctatct ggtgcagggg caggacctca tcttcaatct ggccgggcag gtgagccaca
       61 ctgacagcat ggccgacccc tataccgacc tggagatcaa cgcgcgcagc cagctttcct
      121 tgctggaggc ctgtcgccat ggcaacccga cagctaaaat catcttcgcc agcacgcggc
      181 agatctacgg ccgcccggcc tatttacccg ttgacgagcg tcatcctctg caaccgaccg
      241 acgtcaacgg catcaacaag ctggccggcg agtggtacca tatcgtctac cacaatgtct
      301 atggcttgcg cacggtgtcg ctgcggctga ccaatacatt cggcccgcgc atgcgcattc
      361 gcgatgcgcg ccagacgttc atcggctggt ggattcgcca attgctggag ggccaaacac
      421 tcaacatctt tggcgacggg ttacaggtgc gcgacttcaa ctacgtcgac gatgtgatcg
      481 aggcgatgct gatggttgcg gcccacgatg tggctgacgg gcagatttat aacctcggcg
      541 gcgatgaacc catcaacttg atcaatctgg cgcgattgat gatcgaagtc aacggcgggg
      601 gggatttcgt cctcaaaccg ttcccggatg atcgcaagcg catcgatatc ggtgacttct
      661 acggagacta tcgcaagatc cgcagcaaat tgggctggcg gccgatggtt gggctgcgcg
      721 atggcctgac tcgaacgatg gattattttc gtgccaacct tgagtattat ctttaaccgc
      781 aatgaccacc cttgaaaata taccgtttgt cgatttgagg acagcatatc atcgactgcg
      841 aacggagatt gatgcggccg tggctgacgt acttgaagga ggctggtata tccttgggga
      901 gcaggtgcgc gctttcgagg aagagttcgc ggcgtatttg tcgcgagaat cggttgtgag
      961 cgtagcctcc ggcactgacg cggtgttgct ggctctacgc gcgtgcggag tggggccggg
     1021 ggacgaagtg atcacggtca gccatacggc cgtcgccacg gtcgccgcca ttgaattatg
     1081 cggcgcgatc ccgcgtctgg tggacataga gtcggagacc ctgacaatgg acccacaggg
     1141 attccgtgcg gccatcaccc ctcgcaccaa ggccgttgtt cccgtccatc tttatggttg
     1201 cccggcccga atggatgata tattggtcat cgctcgcgag catggtctgg tcgtcgtcga
     1261 ggattgcgcc caggcccacg gcgcgcgcta tggcgatcgg atggtgggca cgatgggcga
     1321 cgcagccgcg ttcagcttct acccaaccaa gaaccttggc gcaatgggcg acggtggcgc
     1381 ggtagcgact tctcgggccg acgtggctga aaggttgcgc ctgttgcgcc aatacggctg
     1441 gaaggagcgc tatatcagcg acgtgcccgg ctataacagt cgcctcgatg agctgcaggc
     1501 ggccatcttg cgtgtccgcc tgcggcacct ggacgaagag aacgaagccc gtcgtcgtct
     1561 ggccgcagcg tatgatgccc atctggccgg tctgccggtc gtgcgccctg tgcctcgacc
     1621 tgaagaccat cacgtctatc atctgtacgt cattcagacc gagcgacggg atgcgttgcg
     1681 cgctttcctg caaggccgcg ggatcggcac ggccgtgcat tatcccgtgc cggttcatcg
     1741 ccagcctgct tatgagcgcc tcggctatgg cttgggcagc ctgccggtga ccgagcgcgc
     1801 agccgagcgg ctggtgtcct tgcccatgta tcccgatttg tcacccgatg ccccggcgct
     1861 tgtcgcggcc gccatcgcgg aattcttcag acaatagcac ccaacggctg aggtgggccg
     1921 aacctcgatg actgcttctc tcggcaccat tcgtcaattt cttgtggccc atttctccga
     1981 tgaggaattg gatcaatttt gcttcgatca tttctatgag gcccagcagc attttgcgac
     2041 aggcatgagc gtcaatcgca aggcgatggt gctcgtcgat tattgccatc gccgtggtct
     2101 ggtgcccgca ttgctggatg agttacgcaa agagcgtcct gaaccgtacc gcctcgcctt
     2161 cggcggacgg gccagacccg ccatccgacg aattgacatc aatacggcta cggccgatga
     2221 gctgctggaa ctgccgggca ttggcccggc gctggcggca gcgatcatcg ccgcgcggcc
     2281 gttcgacagg gtggatgacc tggcgcgtgt gccaggtatc ggccgcaagc ggctggcagc
     2341 catgcgcgaa tggtgttcgg cgtgatcggt tcccgcgttc agtccgcgcg gccgttgacg
     2401 caaattggac gatatctcgc tgattgtcgg acgatgacac cattagtgcg catagtcact
     2461 gacctgtaat ttacccagcc gataggttgg gatatactac acaagccggc aaattgccga
     2521 gatcgaaaga gagagaaatc gaatgcccac agcattaatt acgggcgtaa ccggacagga
     2581 cggttcctac ctggccgagt tcctgctgga gcggggatac cgcgttgtcg ggatggtgcg
     2641 ccgcacgagc acgctcaact tccatcgcat tgcccacatt caggacaaac tggagctggt
     2701 gcccggcgat ctgagcgatc agatatcgct tattcgcatc ctggaagaat accagcccga
     2761 cgaagtctac aacctggccg cccaatcttt tgtccagacc tcatggaatc agccggtatt
     2821 caccggcgac gtgaccggcc tgggtgtcac gcgggtgctg gatgccattc gcatcgtcaa
     2881 ccccaaaatt cgcatgtatc aggccagctc gagcgagatg ttcggcaagg tggtcgaggt
     2941 tccgcagcgc gaaacgaccc ctttctaccc gcgcagcccc tacggtgtgg ccaaggtcta
     3001 tggtcactgg atcacgatca actatcgcga gagctataac atgcacgcga cgtcgggtat
     3061 tctgttcaat catgagtccc ctcgtcgcgg tcttgaattc gtgacccgca aaatcaccca
     3121 tcaggcagcc cgcatcaaac tgggcctggc cgatgagttg cgtctgggca atctggacgc
     3181 tcgccgcgat tggggctttg ccggggacta cgttcgggcc atgtggctga tgctccagcg
     3241 cgacgatccc caggactacg tcatctctac cggcgatacc cattcggttg agcagttcct
     3301 gggtgtcgct ttcggccatc tggatatgga ttggcgagag tttgtggtgc aggacgaacg
     3361 ctttatgcgg ccggccgagg ttgacctgtt ggtcggtgac cccggcaagg cgggacgcga
     3421 tttgggctgg gagccaaagg tgacatttgc cgagctggtc cgaatgatgg tcgacgccga
     3481 catggaactg atccgcaatg gagaagccgc cgtctagttg ctgtcgaatg accactgatt
     3541 actgaacatt gattactgat ctactgatct tgctatccca ctgaccactg aaatggaggc
     3601 ctgatgacca aaattatatt cggcacagac ggctggcgcg gccggatcgc cgaggattat
     3661 acattcgaca acgtgcgtcg ctgcgcccag ggcttcgcca cgttcttgca ccggcaaggt
     3721 atcgccgagc gcggcgtggt gattggccac gaccaacgtt ttcaggcgga gttcttcgct
     3781 gctgcgtcgg ccgaggtgct ggccgccaat ggcattaagg tgtggctgac tcaaagtaat
     3841 acgccgaccc cggcgatatc ctacgcagtg ccggtcagga atgccgccgg cgccatcaac
     3901 atcaccgcca gccacaaccc gccccatgat tgcgggttta aggtgcgtga tgaaaccggc
     3961 ggggctgtgc cgcctgacga cctgaagcgc attgagtcgg cgatccccga tatcgacggg
     4021 gtgaaacgaa tgaagctcga cgatgccatg agcgacggca cagtggtcaa gttcgatccg
     4081 gcgccggagt acatcgcctt gctgaatcgt ctggtcgata tcgagccgat caaggcggcc
     4141 gggtttgacg tcctggttga ctgtatgtgg ggcaacggcg cgggnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnntactac ttcctggaga ttcgcggcga gcgtggcccc
     4321 atcgtgaaaa ctatctccac gaccaagatg ctcaacaagc tgggcgaaat ctacaacgtg
     4381 cccgtctatg agaccggcgt tgggttcaag tatattgcgc cgaagatgac cgaggtcggt
     4441 gcgatgatcg gcggcgagga gagcggtggt tatgctttcc gcggtcacgc ccctgaccgc
     4501 gacggtatcc tgggcggcct gttcttgctt gacctgatgg tcaagaccgg caagaagcct
     4561 tcgcaactga tcgattggct gtttgcgaag gtcggcccgc actattatga ccgcatcgac
     4621 accgcgttcg aaacgtcgcg gcgcgacgag gtgcggcaac gcgtcctggc agcgcagcca
     4681 aagacgattg ctggactgaa ggtgactgag aaatcgacgc tcgacggctt ccagttcttc
     4741 atggaagatg gcggttggct gctcattcgc ttcagcggca ccgagccgat tattcgcgtc
     4801 tacaccgaga caactcatgg cgacaaggtg caggatatcc tgcaggacgg gttacgtatc
     4861 gccggtttga agtaaacatg gaaaaagcat ttcaggatta ttaccccgac gaagtcagct
     4921 attgttacgg ctgcggccgt ctcaacgagc atggcttgca aatcaagagc tattgggagg
     4981 gcgacgaggc ggtttgccat taccagccgc gagattatca cacggccgtt cccggctatg
     5041 tgtacggtgg cctcatcgcc tcgctcatcg actgccacgg caccggcacg gccgccgcgg
     5101 cgatgtatcg cgagcaagga cgagcgatgg acaccgagcc gccgctccgc ttcctgacgg
     5161 cctcgcttca tgtggattac ctgaagccga cgccgatcga tcttccgctg gagctgcgcg
     5221 cgcgggtgaa agagatcaag gggcgcaagg tgatcatcaa catcgatctg atcgcccacg
     5281 gcgaagtctg tgcgcggggg gagatcgtca cggtgcaagt gcctgatgag ttcatggctc
     5341 gcatcctggg caaagatgga tgatgtctga ttcccccgtt gtcctcatgg acacgcattg
     5401 tcacctggat ttcgtcgcac ctatcgacaa ggtagaagag gtggtggggc gtgcccatat
     5461 ggcaggtgtg gagcgcatcc ttgtcccggc aatagaaatg gataacctgc cgcgcgtgct
     5521 gtcattggct gagaaattca ccggagtctt cgccgctgcg gggatccatc ccaactcggc
     5581 cgcgggctgg cgtgacgaat gggtcgaccg ggttcgcgag gcggccaaac atccgaaggt
     5641 tgtggccatc ggcgagattg gtcttgatta ctattgggac aagaccccaa aagagacgca
     5701 acacacggct ctggcccgcc aattggagct ggccgcagag ttatccctgc cggtcatcat
     5761 ccataaccgc gaggcgagcg cggatacgat ctcgacgctg gctgcgtcgc ccctgaacgg
     5821 cagtgatcga ccgggcgtgc ttcactcctt ctcgggggat tgggcgacgg ccgaggcggc
     5881 gctggagatg ggcttctatc tgggcttcac cggcccattg acttacaaaa aggctgatga
     5941 cctgcgcgcc attgccgctc gcgcgcccct ggatcgcata ctcatcgaga cggacgcgcc
     6001 ctatttaacc ccggaaccct atcgcggcaa gcgcaacgaa ccggcttatg ttcgcctcgt
     6061 ggccgagcgt ctcgcggagg tgcgcggcct ttccctgccc gagatcgcaa ccgccacaac
     6121 cgcaaacgcc ctccgtctct tcggaatcgc ccactaaccc actgactcac tgatctactg
     6181 acccactgat ccactgaccc actgacctac tgatctactg tcccactccc ccactcctcc
     6241 actcccccac catgctctca gtcatcatcg tcaactggaa cgtccgcgac ttgttgcgag
     6301 aatgcctgca atccattgaa gccggaaggg atggcttgtt gctggagatc atcgtcatcg
     6361 acagcgcttc cagtgatgac agcgccgcga tgatccggtc ggagttcccc tcggtacacc
     6421 tcatcgcctg cactgaaaat gtgggcttcc cgcgcgggaa caatatcggc ctgcgagaag
     6481 ccaggggcga ttatctgctg ttgcttaatc ccgatacggt ggtgctgggt gacgcgctca
     6541 aggtgctggt gtcctacctc caggccaacc ctgatgtggg cgcggttggc ccgcagctgc
     6601 tcaaccccga cggcagcgtc cagtcatcgc gtcggcgctt tcccacggcc gcgacgggct
     6661 tcttcgagag cacgtggctg gaaggactgg ctcctggagt tctgcgtcgc tactatgcac
     6721 ttgacctgcc cgatgacacc acggccgatg tggattggct gaccggcgct tgcatcatgg
     6781 tgccgcgctc gacctgggag gcgattggcg gcatggatga ggggtacttt atgtactccg
     6841 aagaactgga ctggtgccgc cgcatcaagg aatccggccg ccgtgtggtt tatcatcctg
     6901 ccgcgcaggt gatccaccac gtcggcaaga gcagcgaaca ggcggccacg gcgcggcata
     6961 tcaacttcaa ccgggccaag ttgcgctatt tccgcaagta tcacgggcgc tttccggctg
     7021 ctgttctgcg cgtgttcctg ttggcgggtt atgcctggca gatggcgttg gaagggttta
     7081 aggggttgct tggcagcaag ccggacctcc gccgccagcg gattcgcgcc tactctgaag
     7141 tattaaaatc cgggttgcgc ccggcgggat attgacttat gcgcgttgga ttggtgaccg
     7201 gggaattccc gccgatggag ggcggggtgg gggcatttac ggagcaattg ggccgtgccc
     7261 tgaaggtgtt gggccatgag gtccacgtca tcacctcgat taaggcccga ccgaaagatg
     7321 cgccgcgcac gctcgccagg cttcgcgaac cgatcctgct tgacttcggt tatctccatc
     7381 cacggatcgg ccgctggcgt tggccgtcga tctcggccgt cgccgatctg gttattcgcc
     7441 atgatattga gcttgtcaac ctgcaatatc aggcggccgc cttcaatatg aacagcgcgg
     7501 ctatctcata tctgccctgg cgactcaagg gcatcgtgcc cacggtcacg accttccacg
     7561 atttgcgcgt gccctatctg tttcccaaag ccgggagatt gcgcgaggcg gccgttcgtg
     7621 ggctggcgcg ccgttccgca ggctgtattg ccaccaatcc ggccgacttt cgccaattga
     7681 cggcgtggag tgacagattg gtgcgggata ttcccatcgg cagcaatatt gccacccacg
     7741 aacccaatca catcgagatt gaggaggccc gcctccagtt gggcttgcgg ggagacgatg
     7801 tgcttctggg ctatttcggc ttcctgaacg agaccaaggg tgcggatacg ctcattgatg
     7861 ccctggctcg cctggatgaa cgttttcacc tggtgtttat cggcgggcag acgggggcca
     7921 gcgatcccga caataatcag acctttctgg gggggatgcg cgatcgcatt acgcaggctg
     7981 gcctcgcgaa ccgggtgcat tggacggggt tcctctcccc ggagcgggtg tcaacgtatc
     8041 tcaccgcggc cgatctgatg gccatgccct atcgcgacgg ggtgtccctc cgtcggggga
     8101 cgctcatggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgatggccat
     8221 gccctatcgc gacggggtgt ccctccgtcg ggggacgctc atggcggtac tggcccacgg
     8281 tcggccgctg atcaccaccg tgccggcaga gatttcgccg gagttcaggc atggcgagaa
     8341 tatgtggctt gtgccggtgg gcgacgtcga tgcgctgtgt ggggcgatag tgactttgac
     8401 ggccgatcca tcgttgcgcg aatcgctggg ccacgaggca agggccttgg ctcgctccta
     8461 tcgctgggac gtgatcgcag gccagacagt cagtctttat tccgaggttc tgggttcgtg
     8521 ggttggcggt tcgctgaaat gacgtagcat tatcaacgaa tcggcgctct cgccaacctt
     8581 cttgaatccg tagcgttcgt agagccgacg ggccgggtta ttcctcgaga cactgagaga
     8641 gacggcctgg aagcgacagg cggctacatc caataggtgg gccagcagcc cgctcccaat
     8701 cccccgcgca cgattttcgg gtaagacggc catacttagc tcagggatat cgtcgcctac
     8761 atagccgtaa cctcgccgtc caccatcaaa gagccgcagc cagattgtgc caatcggttg
     8821 cccattttct tcggcgatga aaccgatgtc acccgcttgt ccccatccgg caacgtagcg
     8881 gctaagctcc gggctgtgga cgatctcgcg aggtggtggt aattttcccg ggggcgtgta
     8941 gatagccaag tagagcgctt cccataagaa tagctcgtcg gccaccatga ttgggcgaat
     9001 ggaaatacta cccttcatga agctcattat acgacctgcc gttatgcttc gccatttaat
     9061 cgtataatcg tagggtatcc atggtcaagc cggttatgac agaacgatga cccgctggta
     9121 caattccctc aatgctttcg cacccacgcg ccgagcgacg tctcctgtcg ctcattctcg
     9181 ctctctatct catcctggga tttacctatg ccctgcttac cccgccgttt gaggcgtcgg
     9241 atgagttgtg gcattacccg atgatacgcc accttgccga tggcaacccg ctgccggtgc
     9301 aggtgttcga tccggcactg gccgggccgt ggaatcagga agccagccag cctccgttgt
     9361 actactatct tggcgcgctc ctgactttcc ccatcgacca gtcggatatg gagacggttc
     9421 ggagacagaa ccctcacgtc gataccggtg ttctcacgcc ggacggcaat acgaacctgg
     9481 tcgtccatga tcccgcggcc gatccgtggt cgggttcatt gctggctttg cgtgtcgtac
     9541 gcctgttttc ggttttgctt ggcgcggcga cggtttacct gacttggcgc atcgccgacg
     9601 agactgcgcc tgggcgtccg gagatcgccc tcggcgcggc ggcgatcaac gctttcctgc
     9661 ctatgttcct gttcatcagc ggcgcggtga acaacgacaa cctggccttg ccgctgactt
     9721 cgctggcctt gttactgatg atccgcatcg tcacacaacg gaaggaaagc taccacccgg
     9781 tgcgctggtg ggaatgggtg agtatcggta tcgttgtcgg gtcggccgcg ctgaccaaag
     9841 agggtacctt ggggctattc ccgatgattt tcggcacggc catcgtggcg gcatggcagc
     9901 aactggttgc cggtagccgg gactcgcgcg gcaacctctc ggccgtcatt tggccggtcg
     9961 ttcgtgggct attgtcggcg gctgtcttcg caattgtgcc cgccctgatc attgcgggct
    10021 ggtggtacta ccgcaatatc gtcctgtatg gcgattggct tggctggagc gccttcattg
    10081 ctgtcctggg cgagcgaggt catgccgctt ccctcatcca gctatggggt gagcggcgcg
    10141 gctttatgat gtcgttctgg ggcctcttcg gcggcgtcaa tgtgtcgatg ccgatgtgga
    10201 tctatgcgac gctcaacgcc atcctgatcg tcagtgtggc gggcttcgtc ttcttcttta
    10261 tccggctgtt gtcgactgaa tggcgcggat ttcgcgccgc gcgggagccg tggccggcgc
    10321 ggctgcttcg ccccatcgag cgtaattttg gcctcatcgt caatctggtc ttttcggcgg
    10381 cgatcgttta tggtttgatt caatgggcca cgacgacgtg gtcgtcgcaa ggacgattgg
    10441 tctttacggc gatttcgtcg ctgtccacgt tgatggcgtt ggggctggcg gggagcgcgg
    10501 atttggcgaa caagctcacg cgcggccgaa tttcgctgcg tccgtggctg attgggggag
    10561 tcgccgcttt catgctgcta gtggccgcgg ccgcgccctg gttgtggatt cggcctgcct
    10621 atcagccacc gactgtgagt gggccgctgg ccgagcgtct tgatgcggag ttcggcggcg
    10681 tcgtgcgtct caacagctac acaatcgaac atgagggaga actcaggccc ggctccgaga
    10741 tacgcgtccg gctggagtgg gaaagtctgc aagccacgga gcgcgactgg agcgtcttcg
    10801 tccatctcaa cgatcccgtg ctgggccatc ccatcgccca gcgcgacatg tacctcggcc
    10861 gtgggctgct gccgaccagc ttcattgtgc ctggtcagca gatcgtcgat gagtatgtat
    10921 taaccatacc caccacggcc gtcgcccccg ctgatctgga gctggtggtc ggcttgtatg
    10981 acttccgcac gggcgagcga ttggcgacgg ccacgggcga ggaaaccgtt tcgctgggta
    11041 tcatcccgct cttgccggcc gaaggggcgt atcccaatcc cgtctcgctg ttcttcgagc
    11101 atgggcttga gctggtgggt ttctcggtcg aaccgcgacg cgtggctgcc ggagagtctc
    11161 tggaactggt ggcctactgg cggccgtctg cccccttgcc cgacgactac accttcttcg
    11221 cccaaatcgt cggccccgac acgacgcgtt atgccgcggc cgacatcgcc ccaccacagc
    11281 cgacaagtca atggacgacg ggcaaggtcg ttgaactgcg ctatccactg acggtcgatc
    11341 cggccgctcc gcctggcgcc tttccgctca tcctgggcct ctacacgatg acgcccgacg
    11401 gtggctttga ccgcttgcag caggtgacgc ccgacggccg cctgactgat gactttctgg
    11461 tgctgacgac ggtgagggtg gatacgccat gacgaccggc catgagccaa tagcagggga
    11521 gcgggggaga aggggagaag gagagaaaga acgccccctg actacttccg gcggtcggcg
    11581 atcggtcgtc ggccgtcagc ttggcccagc tgactccgct ctgtcgcttg gcctgctgat
    11641 cggcgcaacg gccgtgctgg ccggcatcat tctggcgttc ggcgggccga tcgccggcgc
    11701 ggccgtggtc atcggtggcc tggcgacatg gctggtgctg cgcaacatcg aactcggctt
    11761 cttcgcagtg atcgccgtcg tcgccatttt gccgtttgcc acgctgccgg tggacatcgg
    11821 tctgacgccg acgttcctgg atttcgccct ggccgcggca atcggggtct ggctgctagg
    11881 catcgtcacc ggccggcaac ggcggctcat caccgcgccg gtcgccctgc cgctgatcct
    11941 gttcatcatc gtggccgtct tcgccttcat cttcggcctg gccaatggcc cgctgacgcc
    12001 cacactgctg cgcaagttcg ccgagttgtt gctgagtctg gggttcgtca tcgtcgtcat
    12061 cgactactgc cgcacgtggg ttcgcctcga acggctggtg aagtttctgc tgttgatggg
    12121 cgcggcggcg gccgtcatcg ccatcggcct ctggctgctg cccgacgcca cggccaacag
    12181 cgccctcacc gcgctgggtc gcgtggggta cccgggcggc tgggtgattc gctacatcga
    12241 ggaaaaccct gagctatccg agcgggcgat cggcacctcg gtcgacccca actctctggg
    12301 cggcttgctg ttgatgattg gcgcgctggt tgggccgcag gtcgtgactc gcaagccaat
    12361 ctttcgccgc tggatcattt ggggcatcac cgggctggtc ttcctggctc tcatcctgac
    12421 cttttcccgt ggagcgatgc tgggattggc cgccgggctg gggtttgtgg ctctcgcgcg
    12481 ctaccgtcgc ctgatcccct acatgctcgt cgccgcgctg ctgctgctgt tcctgcccgt
    12541 cacgcaggcc tatatcgccc gcttccttga gggcttccag ggggacgacc tggccaccca
    12601 gatgcgcttt ggcgaataca aggactcctt gcgcctcatc gcccggtatc cgctgtttgg
    12661 cgtgggtttt gccggatcgc cggatatcga cctctacacg gccgtggcca gcgtctactt
    12721 caccatcgcc ggtcgaatgg gcctctttgg gttgtcggcc ttcttcggcg tcatcggttc
    12781 cgtgttcgcc tatgccttca gaaatcgcaa ggcggcgcga attgacgaac gccgcgatgc
    12841 cgtctggctg ggcctccacg ccgcgctaat cggcgcgctc gtcgccggcg tgttcgacca
    12901 ttacctgttc aatatggatt tccaccacgc ggtgacgatc ttctggatga tgctggggat
    12961 ggcggtggcg gcgacgcgat tggtgcggga agaagggatt tcctgacccg attcctttgg
    13021 cttccttcgt ttgtagccgg ccgcttcagc cgcgtctgcg cctacttcct ctataaaata
    13081 aaaagcgggg ccgaaatcac ttccggcccc gctccaatct caactcttca atccagggtc
    13141 tagatcgtcg gcaataactc ctctcccgcc tcttcagtcg gcgtcggatg ccgcagcacg
    13201 atctcgtcgt tctccacgtc aatgatgacc gtctcgcctt cctggaagtc cccggccagc
    13261 acggcgtcgg agagcttgtc ctccaccgtc tgctggatga ggcgacgcag ggggcgcgcg
    13321 ccgtattcgg cgtcatagcc gtggatcgcc agccagtcct tggctgcgtc tgtggcttcc
    13381 agcatcagga agtgttcgtc catgcgctcg ttgacgtcgc cgatctggat gtcgacgatc
    13441 tgccggatgt cctccggctg caactggcgg aagacgatga cgctgtcgag ccggttgagg
    13501 aactcgggcc ggaagctgcg cttgagctgc tccatgagct tcttctgcat gtcgcggtag
    13561 ttctcttcca cgagaaccga cgcgtcctgc ttgaagttga agcccaggcc accccctttc
    13621 ttgatcatgt ccgcgccgac gttggacgtg aggatgatga tcgcgttgcg gaagttgacc
    13681 ttctgaccct tggcgtcgga gagatgaccc tcttccatga tctgcagcag caggttgaac
    13741 gcttcggggt gggccttctc gatctcgtcg aagacgacga tggagtaagg gcggcggcgg
    13801 acggcttcgg tcagctgccc ggcgtcttca tagccgacgt agccgggcgg cgaacccacc
    13861 aggcgggcga cgttgtggcg ctccatgaac tccgacatat cgagttgcac cagcgcatct
    13921 tcgctgccga acaggaaggt ggccagcgcc ttggtcaact ccgtcttgcc cacgccggtg
    13981 gggccgagga acatgaacga gccgatcggc cggcggggat tcttcaaccc ggcgcgggcg
    14041 cgtcgcacgg ccttggaaat cgccacgatg gcgtccttct ggccgacgat ggccttctcc
    14101 atctcctctt ccatgcgcag caggcgggcg ctctcttctt cggtcagctg gtagacgggg
    14161 atgccggtcc acatggacag cacctcggct acgtcttcgg ccgtcagcac cggcgaatcg
    14221 tcgccgttga tctgcccggc ccgcaactcg ttcagttgct cctggatgtc ttcttcctgc
    14281 tcgcggatgc gcgtcgcgtc ctcatagcgc ccggcttcca cagcctcggt gcgctgcgtg
    14341 cgcaaatcgc gcaacgcttc gtatgcctcg cgcacgtcgg tcggctgctg cacccggtac
    14401 atgcgcacgc gggaagcgcc ttcgtcgatg aggtcaatcg ccttgtcggg caggaagcgc
    14461 tcggtgacgt agcgggtgga caggaagacg gccgcctcga tggcttcatc cgagatgagc
    14521 agattatggt gctgctcata ggcgcccttg atccccttca ggatttcgac ggtctcttcg
    14581 gcgctcggct cgtccacccg gacgggctgg aagcggcgct ccagggcggc atcgctctcg
    14641 atgtacttgc gatactcgtc gagcgtcgtc gcgccgatgc actgcaattc gccacgcgcc
    14701 agcgccggct tcaggatatt ggccgcatca acagaactgc cggctgaccc ggcgcccacc
    14761 agcatgtgga cttcgtcgat gaacaggatg gcgtcgctgg atttaagttc ctcgataacg
    14821 cgcttcaggc gctcctcgaa ttggccgcgg tacatcgtgc ccgccaccag gctgcctaca
    14881 tccaactgga ggacgcgctt gttctgcagc gattcgggta cctggccatc gacgatgcgc
    14941 tgggccaggc cttccacgat ggctgttttg ccgacgccgg gctgtccgat gagggcgggg
    15001 ttattcttcg tgcgccgggc cagaatctgg atgacgcgct cgatctcggt ttggcggccg
    15061 atgaccgggt ccaaggcgta ttcctgggcc agagcggtca gatcggttgc cagctggtcg
    15121 acgagcggcg tcttgctctt ctctttcttg gcctgttgtt gctgctgccg gcgtggctgt
    15181 tgcggctgcg gcgtctcggt tgtcgagacg gggctttcct tcagcatccg gcgcgtttgg
    15241 cggcggatct gttcggcgct gatgccgaac ttgcgcaaca cgtcaatggc cagtccttcg
    15301 ttttggcgcg ccaaaccgag gagcaggtgc tccgtgctga tatagtgttg ccccatgcgc
    15361 cgggcttctt caaccgcgta ttcgaggata cgcttggtgc tgggggaaag ctctgtcttt
    15421 gaaaatgggg tgcgggtgcc ctggccgccg gatagccgct cgaccatggc ttgcacgcgc
    15481 gtcacctcca gaccgagttc acgaagaaca cggccggcga cgcctccctc ttccagcagc
    15541 aaaccgatca aaacgtgctc actgcctatg taactgtggt taaggcgctc tgcctcttcc
    15601 tgggccagac tcaacacccg gcgggcgcgt tgagtaaatc gttcccaatt tttagagttc
    15661 acagcttttc cacctcaggg aaaatcaata cgaatacagc gcgcaatgac ggcgtatcta
    15721 catcgtattc agcatcgcca ggtcttcttc aatgaatttg tccgacgcca cctggcgcag
    15781 gctcaaaccc acctgacgct gcttcgagtc gagacggatg atgcggaccg tgattttgtc
    15841 gccgggtttc acgatgtccc gcgcctgttc gacgcgatca tccgccagct ctgaaatgtg
    15901 gattaaaccc tccaactggt aatcatcgtt gacgcgcgcg aaggccccaa acttggtcag
    15961 gcgagtgatc tcggcctcga ccagttgacc gacgcgatac agatcttcaa tccgcgtcca
    16021 cggatccgat tcgagccgct tcatgctgag ggcgacccgc tggcggtcct gatccacgct
    16081 gaggatctgg accttgactc ggtcgccttt ttgcaccata tcgccgggct tttgaatgcg
    16141 tttccagctc atctccgaga gatgcaccag tccctccacg ccgccgatgt cgacgaacgc
    16201 gccgaagtcg gccaggttca cgacttgccc ttcgaagacg gcgccttctt cgagcgacga
    16261 gaacagttcg ctcttgcgcg cctggcggat ctcgcgtgag gcggcttgct ccgacaggat
    16321 cagccggtta cgcgggcgat cgacctcgat caccttggcc agcatcggtt gccccaccag
    16381 agattggtag gagcgttggc ggttgtcgcc ctcgatctcc tgcaggcggt cgcggcgcat
    16441 ttgcgagttc ggcacgaagc cgcgcaactg ccccacgctg acgaggacgc cgcccttgtt
    16501 gaagccgacg atttccgttt cgatggcgtc gttcgactcg agaagttcgg tggcgagttg
    16561 ccaatcggct tcttcggccg cgcgacgata ggagagaata atattgccgt tgttatcttc
    16621 cggattcacg acgaaaacga gaatttcttc gccttccttg agtaattctt tcgtggcgga
    16681 atcaaatccg gccgtttcct ggttagggat caggccctct gacttggccc caatgtcaac
    16741 cagtatttcg ttgttacgta ggtcgacaat acggcctgtc cggatttcac cggcggtggg
    16801 aagatacact tcctcgttga ggagaaagtt catggggtgt tcggcgtcac caaatgcgga
    16861 gtcaatcatg tttctaaata tatccatctg cgccggaagg ccatcccccc tgcgcccaat
    16921 tttgagcgat tatacttcct ttaggccgct tggcaaagaa cttaatctac tgtataacga
    16981 tccggggatg gctggaaaag caggttttac cgtcggcgcg cggaacgccg gagcgccgac
    17041 atggctcttc cgactgtttc cgtttctcct tttttccctc agttttctgc cccggctcgt
    17101 ggcgatcggt cgctacgtga cgcctgatga gccgacgtgg gtttatcgct cggtccagtt
    17161 ccgcgaggct ttgctggccg gcaactgggc tggcacgttg gtcgcaggac atccgggcgt
    17221 catcaccacc tggctcggcg caatcggcgc gtcggttcat atgctgtttt ccgccgacgt
    17281 cgctgaagcc tattcctggc tcttaaaagt ggcttttctg acgcccgaca acgtggcggc
    17341 attcgaacgg ctggcgatgc tgctgagcag cggtcgggta gcgatcgcgc tcgtcaatag
    17401 ctttggcgtt gtcgccatct gcctgctggc tcgaaaatta tggggcatga aggtcgccat
    17461 tgtgggcgga ctgtttctcg cgctagatcc gttcctgctt ggattatcgg ggctgctgca
    17521 cgtggatggg cttagcgcta ctttcgtcac gatctccctg ttgtcattag cggtgggctt
    17581 gcggtcgggc gcacgggacg gctccgtcaa tcataaatgg atggcactgg ccggatttgc
    17641 ggccgcattg gccgctctta gcaaaacacc ctcacttctg cttttaccgg tgtcaggtgt
    17701 ggcgctcctg tggggtcttg tggctgggcg ggatacccct tttggcgcgc ggctggcggc
    17761 cttccttcga tgggccatcg tctggggggc gtcgttcgcg gtcacggttt tagtcctgtt
    17821 tcccgctgcc tgggccgcgc cggctgcggt catcggcacg ctcggtggca gcgccaatcg
    17881 acatctggac gaggcgttgc gtcagacctt tttcatgggt gaggctgcct tcgtccacgg
    17941 tccgcttttt tacccgattg tgctgctatg gcggctcagt ccggtcgttt ggttgtctct
    18001 cccggcgctg ggcttcctgt tcattgttcg caagcggatc aaggacgccg tgtcgcataa
    18061 taggcccacg gtgatcctat tagcactctg gatcattcta tatcttggga tgatcactat
    18121 ggcggccaag aaattcgacc gctatgttct gcccgtcgtg ccgtcgctgc tgttgctggc
    18181 ggccatagtc tgggttgagc tatccagagt gcggccgcga gctgggcgcc ggcttctggc
    18241 ctgcgctgtc gcaattcagg gtctcttcct gctcgttcat gcgagctatc ctttggcggc
    18301 ctataatcca ttggtagggg gttcccgcac ggcgctgggt gtattgccgg tcggttgggg
    18361 ggaagcgatt ggcgcggcgg gcgcatatct tgaccaagct gtgcccgacg catcgcagtc
    18421 tcgcgccatt gcgcctattg ctccggcttt ggcgcccttc ttctccgggc agacgctcgt
    18481 tgagggatat gacgatccgg ccacggccga ttacgtgatc gtcactgccg aggagagaca
    18541 attggattcc atcggcgtgt ctgccttgac ggatgggatg gaattgcagc gaaccatccg
    18601 ttttgcagga atcgaccagg cgtggatcta tcgaaacccg ttgccattgc cgccaattac
    18661 tcccgaagag ttggatgctc cggccacttt cggagagcgg atcgctctat tggcatttag
    18721 ccaatcagtc gatgatgacc ggattgattt gcatacgaga tggcagcgcc taatctcgac
    18781 caacgaagca ggtcgataca cgctacgcat agtcattaag gatggggctg ataatctttg
    18841 ggccagtcaa gaaaccccgt tgctgaatga gacctatttt tatccacccg attggccgca
    18901 agcgcagacg gagatggttc gatatcagtt ggaactgccg ccgggggtgc cgcctgggga
    18961 gtaccaggtg gtgttgtcgt tgatcgatga cgcgacagcc ggccggctcc ccgttcgcgt
    19021 gggaatagac gattttcatg gcgtagccta tgaggcgggg cggtttgatc tgacgttgcc
    19081 ggatgcgatt gtttccgcat cgcgcatgca gattgttgtg gctgaggacg ccacctggct
    19141 ggacggccgc ttgcaactgc tcggccgcga ggagatagcc gccgaggcgt tggcggggag
    19201 caggttgccc gttcatttgt tctggcatgc gccgcagggc ggactggaag cgggacttgg
    19261 acttcgctgg cttttgcggc caggtgacgg ctcggcggat atcgaggtcg ataccatcgc
    19321 cttaagccgc ttcgatacag ggctttggcg aacgggggaa tcgatgcagg agatatacca
    19381 gattcctctt ccccccgatc tgccccccgg ctcctatcaa ttgctgcttt cgccgctcac
    19441 gcaggatggc gatcttgccg gtgacgcctt gtcgttggcg gaactgcaga ttaataacat
    19501 cgaccgtcta tatgagttac cgtcggagct gccggtcgat ttggatgtgc gctgggagcc
    19561 gctaactctg gaaggcatga gtccggcgga gatatcgact gtcgcgggtg aaccggtcga
    19621 ggtgacgctg ttttggaaga agcagcaccc tcatggagat gtgtactctg tcttcattca
    19681 cgtggtcgat gaagcgggga atatcgtcgc tcagtccgat cattggcccg gcgggttgcc
    19741 gacaaatatc cttgatgtgg gtcaggtcgt tgtcgataaa gcttccatcg aaataccggc
    19801 gacgacaact cccggttcct atcgaatgcg tgtcggattg tattcggccg aaagtggcct
    19861 gcgtctgccg gtgattagca gcgcgacagg ccactcaact gattatgtga cgctgcccgt
    19921 cgcgctggac gtcgtttcgc cttaggcaaa actgccggca ccgcctattc tgcttttggc
    19981 gtaccgcgct tgcgcgaggc taaagccagc acaattccca cggcgattaa tgccaggcca
    20041 accagcggaa ggagcaggtt agtcacactt gtcggcgatg tggcccgcga tgcgctggag
    20101 ccacgcgaga ccatacctgg cccggctgca ctatcgcccg tttccccgcc ggctgccggt
    20161 tgtccctggg atacaggcgt ggcgacaatt tccgccgaca ccatatcggg ttgttgtgct
    20221 tcgctttcag gcgcagatgc gccggtctct tctccacctc cgggcgcgac gctcatttcg
    20281 gtagcggttg gggttgccgt aggtgtcggc gttgggacgc aggtcagcag cggcaagggc
    20341 gtcggattcc agggaatacc gggtgtgggc gtcgcgccgt tgatggccgg cggctcggcg
    20401 atggggacaa gtgcggtatt gccgaactct tccacctcac gatcctgaac ccagccgatc
    20461 atgttgggct ttaccaggat ttgccaccat tccgcgtaag ccgcgcggcc agtgatcggc
    20521 cgcatttcac caacctccag ggtggatacc gggccgtaat caacgccagg cccaccgtag
    20581 atgatcaatc ggccgagcgc ccggatataa ggtgtatcgc tacactcgcc ggacccgccc
    20641 ggattcaccc gaattgtacc ccccagaacg actgtgggga tgggggttcc caaaccgctg
    20701 ccggtgccgg gaacaccggg cgcgctggat ggagaaggct gcggagagcc tgatcccggc
    20761 gcgctgggtg atgtccccgt cggcgaagga tttcccggat tacccggtga gccgggatca
    20821 cctgatgtcg ccgtgggttc cgcggttgcc ccaccggggc cgggtgttgg cgtgcgcgtg
    20881 ggaaatgacg gatcgaaggc ctgaatcact cgaggctggg aggacgccag cgcgatcaga
    20941 agggcagtga ttaacgatac ggtcattaat acatgttttg gcatagcatt ctcctccgca
    21001 cgatatatta tcgataccgg atggcgacga gttcatttgc cttcaaattt agctcaaaac
    21061 tgatttgcgt tccatcaata gtgaccggcg tgggcgatgc gtccagcatt gtcgcgccgt
    21121 cgggcaaatt caccatgatc cgcagcctct catctttcat gcccggctgt ttgcgaatgt
    21181 ccagttgata cagagattct cccgatgcca aaggcacgat gatccctgcg ggcagctggt
    21241 aggtgaatgt tgtcgtcact tcttcccctc gaggaaccag cagaaagttg gcgaatgccg
    21301 ttagccaggg aatatcctca accatctgcc cggcgctgcc ccaagttatt ccactgtaga
    21361 gggtctcacc cgggaccacg tgcctgctgg aatccagcag cgtgcttccc gccggtgcgt
    21421 agacgcgcaa gtagctccag tagcattgat cggcgatagc caggtactcg gccgcctcct
    21481 cgaattcctg atccacacct tgatagcatg gttcgcctgt tctttcgcct gtgtggtgat
    21541 aggtgattgt caggtgagcc tggggctgtg cggcaagggt gatggcataa tccagagcgc
    21601 gctcaatgaa gatgttggcc ttgttgtagc ccatgttggt gtccactacc atgaggaaat
    21661 cgccgggtgg ggtctgaggc aggctgccgt cccacccgtt agcggtcaga actgcgctga
    21721 gttgcggatc gcgcatgtag atggaaagat gtcgatcttc ggccgcgccg gccaggtttg
    21781 cggccagctt gagcggatcg atcgaaccaa aatccgtctc gatcttggcc tggatggccg
    21841 ccgcgaatcc gcccagaaat gccttgcgat ctcgcaccca ctcctggatt tcctgccctt
    21901 cctggatgtc gcgcgcctgc cgcagagtct gcagcacgtt gtcggcgctg attcgttggt
    21961 ctgtgcccgg aatcggtacc gggccggtgg catcaaccag cactctaaga aactcctgat
    22021 cgatggcaat
//
LOCUS       DAOMQG010000019        21136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1077_length_21136_cov_5.207106, whole genome shotgun sequence.
ACCESSION   DAOMQG010000019 DAOMQG010000000
VERSION     DAOMQG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 21136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21136
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1077_length_21136_cov_5.207106"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLC06_02390"
     CDS             complement(<1..193)
                     /locus_tag="PLC06_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89323.1"
                     /translation="MIPQINLQIPPNRLSSPHDFGMRVSPSPAQSPWPAAAPTRPIPG
                     DLSVGRQTFQNLNHERSAIL"
     gene            407..1408
                     /locus_tag="PLC06_02395"
     CDS             407..1408
                     /locus_tag="PLC06_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89324.1"
                     /translation="MICTNCGKENPDNNKFCLQCGRPLAGGAARPTATQTSPRRSLGR
                     LPLLIGGVVALVALAAAAWFLLPRLTGGGGGREMLLAAPNRDGEVDLSVLRLGDDLTK
                     AIVVAENVTTTSSTLFDYARPGEPGRLTSDGIYGNFLPDDKRLVFFYSKDGKTELMEY
                     ALGAEAPTEIISDENAQYFAGPMPNSRDLFITEYGSSDTRCYVAAPGDKAERVARADR
                     CGLIPERTRLLTTDYNSKNELTLTVADPDGTNETTLLDDVATDYANTSADASHIAYLN
                     STDDGQSAVLLDAAGQTLFESDTFSAFNGYGFAGASDTFYFVGANEDGEWELYTSGR"
     gene            1381..2559
                     /locus_tag="PLC06_02400"
     CDS             1381..2559
                     /locus_tag="PLC06_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HRO89325.1"
                     /translation="MGALYLGPLIAARALHVQSAGKEPMLAVLTADEDHAGEVTVFNL
                     ATGTSAKIASGDNLMIAQIADPPRLLIREETDGDLKLTAVEWSGANPVTLFDDSDRLL
                     GNLQILPGDKRVLLSLYSEGLFSLYVAPLDGAPGYFILEDWAEFELLDQSGDTLLLAG
                     REDEGDDPILYAVTLAPEAKLIELDDSANNYPYAVVAPDGRSALYNAITGDRLGDVVV
                     RQAKLDGEERPVDLYEEQMLVAARWKPLRFTDVFWRDPGALAQPAAVGIAGTEAGLIV
                     AYEVYDGRIDQQALFDLGGNIGEGNGRLYYFDGFQGQDVQFDIYGGDSINNSSLDPAA
                     GLLDESLNLLADNDDGGSGSDSRLTYTLPQDGRYYLVIVNSGGGYDDNFTYQVSMSYP
                     "
     gene            2602..4377
                     /locus_tag="PLC06_02405"
     CDS             2602..4377
                     /locus_tag="PLC06_02405"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HRO89326.1"
                     /translation="MYCLNCGTQLADTAKFCHACGTPTQKTAPPPPSPPQAATGWGTP
                     PPPPPASPKEQEGGTRRLLGCGVALLGVLLLVGLGLLAIYFLLGLHRDSGITELAPDD
                     AAAVVVIRPGLLQLNQLRDTDRLMGSAAALAPLIAVPGLPDFAFSVYDDYGEMLQNAN
                     INPSEDVLPWIGREVALAATDADTNAVVVAAAVRNETRANAFLADLREQLEDEGVEFD
                     ESNHGGVTITEIVGPDFYTPLAYAIADNRLVLASSREALEDALDRANGRGRTLSDNDA
                     YHDAMAAQPRNRLGTVYIDPAALGTAPEFEALRWVAGSASLIGKGLRFGYRLGFDRNA
                     LDGAQRAWMEQGGIDNRMADRIPDDTMFYLAGGSLAETLENVASQAPDFEEALGEIQQ
                     DGELSGLYDLLEMMTGEFALAVSRDDTGLLAEMTGEPFGLLIVSQADDGDTAHEALDA
                     VFDDIARQAGANYETNEIGNASVGFLDAPFIGPFLGYGVDGKAVILGTSEDLIEKALE
                     PSDALADAPRFKDTMSALPGNGLLYFYLDADAIDRLINVLGIVSETSEDYTRRIEAIG
                     LAVEPLDRGGVMNAELFFLTERPRR"
     gene            4432..4869
                     /locus_tag="PLC06_02410"
     CDS             4432..4869
                     /locus_tag="PLC06_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRO89327.1"
                     /translation="MATIICENCGTENPDNVKFCKQCGNKLPITVPAAPAQAPSPVSG
                     GQSAASGVQMEPRYKALRGIASLLRTIGFVLAVITIGGGAISFLTLANDSLLVAFGSL
                     ILSVISGLLTYLFFLVMSELVSVQLDIEENTRRTAYFLEQRSR"
     gene            4959..5648
                     /locus_tag="PLC06_02415"
     CDS             4959..5648
                     /locus_tag="PLC06_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89328.1"
                     /translation="MNSLVDRFKSGAGKAAFEADKLRRVTSAQSAIKPLRGEADRLYF
                     EMGKLAYLLYTQGGVSQPELQTVGERLAAVQAQIAGAEAEVERIRAEEYVEPLPSAVS
                     QTGLICPNGHGTLMGGASFCQTCGAAGVSPAPMPQAKATMPCRRCGNALDLDARFCPS
                     CGTPVERCVNCGEALPADALFCAECGTPVAVAAPPRTTPPASAPPASAPFAPEWLPLS
                     AEEDTDTKSNT"
     gene            5651..6751
                     /locus_tag="PLC06_02420"
     CDS             5651..6751
                     /locus_tag="PLC06_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89329.1"
                     /translation="MAQQPCPNCGFINTGNGPFCGNCGRSLAESAARDESTTGLCPHC
                     GAENPTGAVFCGSCGLSPAVDQPPVEPHRPARRYLLPLVLLAGLIFLGAAAALFLLPR
                     LGQDRASAALPDNQSSTAEGSSVAGGEEGATDLAPSDEAPSDEAASDEAASVAEVSSV
                     ITTTTSAPTVTPFPTGTLRPTPTPLPTITSQPPTPLPPPTSVPPTAIPLPTTVPPTAP
                     PSVACQSSPGDRWGPTLWARYQNELGCATTGEIHPNSAYQYYQRGMMVWREVPDLVYV
                     LYNDGSFDVFSSPGPDGYFHSDWLKGSFGYLWNNNATVRDRVGEPQAAEFNTTNFAVQ
                     DFAGGTIIYFLENDARNYVLFNNSGTWISAQQ"
     gene            7017..9098
                     /locus_tag="PLC06_02425"
     CDS             7017..9098
                     /locus_tag="PLC06_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89330.1"
                     /translation="MQWKPARRSRRGCWLVLLILLVLGPAIGAIFLLPMLGIELPEPV
                     AGWVGGGAEPSPTRRDEADTPTAEPEQTIAPSATSRGSVIADLPTVTPFPTGTPRPTL
                     TPLPPGMTASTDAPLQPGAAPATPIPPTPTLPAGAFTLAGASGVVAINQPWEQDGVTL
                     TARTIDIRSQEDGDAAAARVWFRLINKTGERILIEIDWNNIHLEDSFGTRYVDYSGGG
                     TTSEWIEPGANFDFDRHYTTIVEQPSRVPGNAAFVQVVVDSFSRVQNARWQFDINPAL
                     TPISPPPAGESKAVGEVWEKNGLTLQLADLRIHDDQNGDAAARAWFALTNATNQEILV
                     DIDFGRIYLLDSFGRRFGDWDGGGLMTQTIGPGQTIEFDRSYSEMSGRFSRITRGATF
                     VTLVVDNTAGQGTALWTFEIDSRLSGSAPVAPLAINQPWEQDGISLTARAIEIRGQDD
                     GDAAAARVWYRLVNKTGQWLLVDIDWSTLHLEDSFGTVYGDYYGQELTSFWIRPGAIH
                     DFDRYYSAIVEQESRIPADTRSVTIIVDAFSRIKNARWQRTMAPELVAQDPPDDSQAL
                     AIGDGWEQDGLMLRLTDLEVRSESDGAAAAFRAWYELVNTTSRPILVEIDYGYLSVTD
                     SFGGRFGDWDGGGLTTRWIGPGESLEFDRHYSEMAGQAGRIARGSAFVLVRFNNEAGI
                     PSGIWRMDITR"
     gene            9121..10686
                     /locus_tag="PLC06_02430"
     CDS             9121..10686
                     /locus_tag="PLC06_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89331.1"
                     /translation="MSLNACPHCGFTNTPGAAFCGRCGQKLASLPPVEQDYCPYCESP
                     NPPGTPYCGHCGHSLIGPTVTTATIPAGERVCPICETANAADAAFCGNCGHAFIGGHA
                     LTGDHALTGGHAFTGGKDDYPYTVPESPLASRSRWLWLLVGLSALVLVCGGSAILLSM
                     NGGRLPAAIAGLLTDPTATPPNPTPESAEPTPETTDTPQPSPVPTLTAVPPTAVVLPT
                     LTPLPTPVPTPVPTSAPTNAPSPAVPPAPIRGSDEIVFQSNRDGDFEIYIMNTDGTNQ
                     RQLTINTIDDRFPRVSPDGRRIVYQSGRGANAEIYVMNRDGSSQQRLTFNESLDDLPA
                     WSPDGRQIIFVTWISRTDSELFVMDADGSNLRQITDTSTDEGHVSWSVNGQLVFNGTE
                     AGTSNYQLYLSDADGGNRQRLTNSRIDEWSPEWSPDGSQILFLSERGSSVNSGIYIMD
                     AATGEARPIYNHEDSRILEWGAVWSADGRQIVFVMDQLDDTANIYIMSADGGSPQLVT
                     ERGGYPTWATGSR"
     gene            10727..12121
                     /locus_tag="PLC06_02435"
     CDS             10727..12121
                     /locus_tag="PLC06_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89332.1"
                     /translation="MAERIKCPHCGFDNASKARFCGNCARPLTHQFSPPPAADADAVY
                     CPYCESPNPPGAPFCGSCGRQLTASQPVPASAACPHCGQANPVGATFCGSCGRSLTYD
                     EEDKRSSRGRLIGLMAIALLVFLGAVALFALPALGVPLPGALAGLTGAEPTHTPRPRP
                     AATEMEAATAEPIPPTAAPAATAAPAATALPAATAASLPTLTPFPTPTPRPLPTATPT
                     PIDTATPDAGPERLVLGQTGRGTPIEAVRFGNGPQTVMFIGGLSAGFAPSTVALAEAA
                     VDHFSRNPTLIPENLTVFIVLSASPDSPVAPGDYRGRLNANGVDVNRNWGCNWAADTR
                     WGGETKRGSGGTSAFSEAESRILRDFIKEKDAAAVIFWQARAEGGLVSPGGCGERVEV
                     SAALAGTYGLTAGYRVENFEDLTRQILNGDASNYLDSVGVPAASVLLPNYSSSIDWQN
                     NLDGIMAVLNSYAD"
     gene            12111..13325
                     /locus_tag="PLC06_02440"
     CDS             12111..13325
                     /locus_tag="PLC06_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRO89333.1"
                     /translation="MPTRRWIRAVLLLLPILLLFLQAGCAEIEWSAVTFPPVPTDVTG
                     PANTLLLRESRSPAEEPLRRVTPTEPATPSPAADSPTMISAIEGLLPAATRPTAIADG
                     KVEPSPLPPPSPVPTVPPVPTVPPVPTVPPVPKATPIPGPTTEPTAGPIGDSAGGRPI
                     TGYRFGHGPTPIVLVGGMHGGYEWNSILLAQTFFAYFTAHETAIPASISLYIIPNANP
                     DGLAVATGKETADLQLRPTDIITPTLAGRFNANGVDLNRNWNCQWAEQGVWRDQTVSA
                     GTHPFSEPETASLSRYFLELQPALVIFLHSTAGGVFPSGCPEMDPESAAAAVVYGLAA
                     GYPIYPSFNYYKVTGDAGDWLSTQDIPSFTVELTTKDDIDWEQNLAGLQAVLEQFHTP
                     EGVELEDGQIYE"
     gene            13318..14799
                     /locus_tag="PLC06_02445"
     CDS             13318..14799
                     /locus_tag="PLC06_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89334.1"
                     /translation="MSKNQIICPNCKFPNTVTAKFCGRCGHGLAAAAPVEPPPPPTDS
                     VYCPYCEMPNPAGAPFCGNCGRALAAEPPAPPAGGIHCPHCEAPNPAGATFCGRCGRS
                     LGTAIHSDEAEPSGRGRWLPLLLLAAGMVFVLAAGAFFIVPLFQSRATPPANGVANEG
                     STATANTGAVAELETETATPTAVPSITPSPTATATPWQTVTPEPTITPTATPIATPTD
                     TPPSIMPLTGNGLMQLSFGSERDYTPSLSPDQSRLIFSSELGSSWQLVEGDVNGSGQG
                     LQLTFGAGNNQAPHFSPDGRTLLVSSDRDGDHDIYLLDSGSGAEIAQLTDIPGDEYFP
                     RWLADGSGFVFSWMANDVEAIYLQRLDGAQTELVRSTTFDGFAWPSPDGRQVAFYSGR
                     DGDYEIYLMDIDGGNPRRLTVSGGRDASPTWSPDGRWIAFESARNGDYDLYVMRPDGS
                     DLRQLTSGSVNDWFPTFSSDGQWLLFQSDRAGEMDIFRMPFAP"
     gene            complement(14796..18314)
                     /locus_tag="PLC06_02450"
     CDS             complement(14796..18314)
                     /locus_tag="PLC06_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89335.1"
                     /translation="MIRTNRVKLLRLAPIRPLIPAFFFCLLVLSVSVQAQTEIGPEAV
                     WQPGFSAAEDINACRQDAGCSPAGIMAGLGASAAAIGFAESRGFFDYLSDFQESGVVD
                     LGTIFHPLAANDNGSYAMLNGNPPFVSTRETFFPDLRTIPGYEALLAQYPQAILWGGD
                     VGSPQAMALPGGGQRFTFVFQIVDGCHACGYVGEALVAYDFDAAGNYLGITYLDPAST
                     PPPVGSASAGQFAYIEPNGGKWETQGSIWLVNADGSDPQQITNSDTDCCLAWSPDGSL
                     LYFIRDARLKDAPETASPGTIMVYDFRTGQERVIDTPDMNLFDVLALSPDGRSLAFSS
                     SEWVDIDPWGDVHKGCLYVMNIETGEAILVDCEAPGAIYSISLGADEVIAVEVGFYEG
                     SRIAFYDSPVSPRRYDDRICCSDPKYAPNGTSLFTNGSDYGSLSSSLIYYESLSASPV
                     VLTEMPNELGSIGWHDVSPDGSSLIYVRGQEIEVMDLVTGAITSVGITGYGPVFRPDY
                     VEVTVDLTALLDRKSAVFEQLETTGYQTSAGVLYPDPIAAFDETAARALIDGLRAADP
                     ASVSPEQAAALERLVVQEEALDPLLDDYTVLASDQADVAVDLAGMATGTGLLAAKAAG
                     GLQAAIADLLMKVIEDFIKLLLHFIDEEDLRGGMSDSIGLAITLLDVYADNVIDAGDA
                     SAMAEEFLERAQDDGVRALAFRELIPKFTGAVQPVVDQGVNSVTGEANPIWTVGGRTE
                     SAQIGLDGLTEQSAIMRELAHDVYTNSLGRGHDVNELLKDIVDLALLGTKNPIGLIFS
                     IQTRLQQVLIDTAATSVLSGAMTCTRDAALLSGEFAFQPDRPVYVCEVPVPLDLGDFF
                     RRLFTEKDGGKHYVGRLAPPAQSTMPEFDAALAGYRAAIAALSDALNAADPDAVRART
                     DELLAATRALDEEAMPVMARLGATAEGATAEAEEGAMALGTAITFARLDALFTLMAAD
                     AWLADPEAADAAAIYDVVLQAEENADLLGRVVAQVPLPAAPDAALTVVDAPAEWAVVV
                     GEATEIPIRVINAGGRPFSGARLMLEIDGQTVAEVAVPDLGPGEAAELSPVYVSSEPG
                     RLRVTARAVAGDRSDTRTLTLVAQPSAEAADGVAPIVADGTVDETEPPIGGNAARPIL
                     FGVMAVSGAVFLLSLGALLLRGRRKP"
     gene            complement(18324..20447)
                     /locus_tag="PLC06_02455"
     CDS             complement(18324..20447)
                     /locus_tag="PLC06_02455"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRO89336.1"
                     /translation="MFHVSGMDSRFAGMARRSIPLLAFLLMMGLLSAAGCGAGLGANW
                     PGEFSPAADSLPAPSPVAAPTRPLPPPVVATGVGMPVTILSETEEVAGRAAYSFAPVI
                     MRRYADLGCNEERGGEAATVTGEMKQWYPLTLAVRGPVVDEADESPNPFLDYRLQVRF
                     LGPSGQAYDVPGYFAGDGRGNGAGDVWQVRFSPDEAGAWRYCVSFRAGESVAVDADML
                     AGAPLPFDGRTGEFMVTDRDPEAPGFLKWGRLEYTGQHYLKFRDGPYWIKGGTDSPEN
                     FLGYAGFDNTIDQGGIIPNFLHTYAPHVGDWREGDPDFAGPNGDMDGRGIIGALNYLS
                     EQHVNSIYFLPMNLGGDGQDSYPFLSPDGSPGANTHYDVGKLAQWDTVLQHAQRMGIA
                     LHVVLNETEQANRRWLDGGALGVERKLFYREMVARFGYLLALKWNLSEENVFSAAEIG
                     EFADTIRAYDWAGHPIAVHNPFDSFGQYQQLLGDSRFPTTAIHYRIDEAGQIVERWRE
                     LSRSAGRPWVVDMDENNPAGVGLGPDNAGELRKTVLYDVYFSGGNIEWYAGYYDLPPG
                     GDVNLEDFRTREAMWRTMWYARRFMEDNLPFWAMEPADALLSGESEAFGGGEVFAYHD
                     EIFAVYLPAANPSGVLQAAAGREYRLRWYDPRTGEFAGEAVSVVASAEGLPLGAPPHS
                     PDGDWVVLVEGIPKIEATPLPVAYP"
     gene            complement(20451..>21136)
                     /locus_tag="PLC06_02460"
     CDS             complement(20451..>21136)
                     /locus_tag="PLC06_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89337.1"
                     /translation="RSADSDGDGLSDGQEVQNYSTDPNNADSDGDGLSDGQEAQNYST
                     DPRSADSDGDGQSDGQEVKTHNTDPNNANSGGSQSGPGGNVAVVEPQGSEPQEKSRIT
                     SFQLINPTTGRAIGAIEDGDTISLRQSGCGASGACYLNVEAIVDGADVASVIFMFDGK
                     PIDINGRSLENTAPYYMAGDINGDPQGNWNWARYVGGQHTIEAQPCDRDNGEGPCTLP
                     LTVTFTVTR"
BASE COUNT         3953 a         7174 c         6324 g         3685 t
ORIGIN      
        1 aaagaatagc cgatcgctca tgattcaggt tttggaaagt ctgccgcccg acggacaggt
       61 cgccgggtat cggtcgggtt ggtgcggccg cgggccaggg tgattgggcc ggtgacgggc
      121 taacccgcat gccgaagtcg tggggggatg ataacctgtt cggggggatc tgtaaattga
      181 tttggggaat caccagcaac aaatttcatc aatcaattgc aaatcctttc taatgatcct
      241 ataatgtcgg ctgttcgatc attactcgat tgccgaagcg acggattgtt tccgccgaat
      301 cgccggtacc ggcgcatcaa tcagtcatca taccaacaac cgcataggcg aggacgttag
      361 ccggtaccgt ccgctttcaa tttgtgctct gtgacaggag gaacgaatga tctgcacgaa
      421 ttgcggcaaa gagaaccccg acaacaacaa gttttgttta caatgcggcc ggccgctggc
      481 cggcggcgcg gctcgcccca cggcgactca gacatcgccg cgccgttcgc tcggccgcct
      541 gccgctgctg attggcgggg tggtggcgct ggtggcgctg gccgcggccg cctggttcct
      601 gctgccccga ctgaccggcg gtggcggcgg ccgcgagatg ctgctggccg cgcccaatcg
      661 cgacggcgaa gtcgacctgt ccgtcctgcg gctgggcgac gacctcacga aggccatcgt
      721 cgtcgccgag aacgtcacca ccacctcgag cactctcttc gattatgcca ggcccggtga
      781 gccgggccgc ctcacctccg atggcatata cggtaacttc ctgcccgacg acaagcggct
      841 ggtattcttc tacagcaagg acggcaaaac ggagctgatg gaatatgccc tgggggccga
      901 ggcgccgacc gaaatcatca gcgatgaaaa cgcccagtat tttgccggcc cgatgccgaa
      961 ctcgcgcgat ctctttatta cggaatacgg ctcctccgac acgcgctgct acgtggccgc
     1021 gccgggcgac aaggccgagc gcgtggcccg cgccgacagg tgcggtctga ttcccgagag
     1081 gacacgcctt ctaacgactg actacaacag caagaacgaa ctgacattga ccgtggccga
     1141 tccggacgga accaacgaga ccacgcttct cgacgacgtg gccaccgact acgccaatac
     1201 atcggccgac gcctcccaca tagcctacct gaatagcacc gacgacggcc aatcggccgt
     1261 tctgctggac gccgccggcc agacgctgtt cgaaagcgac accttcagcg ccttcaacgg
     1321 ctacggcttc gccggcgcgt cggacacgtt ctacttcgtc ggcgccaatg aagacggcga
     1381 gtgggagctt tatacctcgg gccgctgatc gcggcccgcg ccctgcacgt tcaatcggcc
     1441 ggcaaagagc cgatgttggc cgtgctgacc gccgacgagg atcatgcggg cgaggtgacc
     1501 gtgttcaatc tggcgaccgg cacatcggct aaaatcgcct ccggcgacaa cctgatgatc
     1561 gcgcaaatcg ccgacccgcc gcggctgctg atcagggagg aaacggacgg cgatctgaag
     1621 ctgaccgccg ttgaatggtc gggcgccaat ccggtgacgc tgttcgacga tagcgatcgc
     1681 ctgttgggca accttcaaat tctgcccggc gacaagcgag tcttgctcag cctctatagc
     1741 gaaggtcttt ttagcctcta tgttgccccg ctcgacggcg cgccgggcta tttcatcctg
     1801 gaagactggg ccgagtttga gctgctcgac cagagcggcg acacgttgct gctggccggg
     1861 cgcgaggacg agggcgacga tccgatcctc tatgccgtca ccctggcccc cgaagccaaa
     1921 ctcatcgaac tcgacgactc ggccaacaac tacccctatg ccgtcgtcgc gcccgacggc
     1981 cgctcggccc tctataacgc catcactggc gaccggctcg gcgacgtcgt cgtgcgccag
     2041 gcaaaactgg acggcgagga acgaccggtc gatctctacg aggaacagat gctggtagcc
     2101 gcccgatgga aaccgctgag gttcaccgat gtattctggc gcgatccggg ggcattggcg
     2161 cagcccgcgg ccgtgggaat cgccggcacg gaggccggcc tgatcgtcgc ctacgaagtc
     2221 tacgacggcc gaatcgatca gcaggcgctc ttcgatctgg ggggcaacat cggcgaaggc
     2281 aacggcaggc tctactactt cgacggcttc caggggcagg acgtgcaatt cgatatctat
     2341 ggaggagact ctatcaataa ttcatccctg gacccggcgg ccggcctgtt ggacgagagc
     2401 ctcaatttac ttgccgacaa cgacgacggc ggcagcggca gcgattcacg gctcacctat
     2461 accctgcccc aggacggccg ctactatctg gtcatcgtga attccggcgg tggatatgat
     2521 gacaacttta cctaccaggt gtcgatgagc tacccctaga cacgcgcgcg accgaatcac
     2581 gaaccaacag gaaggtttac gatgtactgt ctcaattgcg gcacacaact ggccgacacg
     2641 gccaaatttt gccacgcctg cggcacgccg acgcaaaaga cggccccgcc gccaccgtcg
     2701 ccgcctcagg cagcgacagg ttggggaacg cctcctcccc ctcctccggc ctcgcctaaa
     2761 gagcaggaag gcggcacgcg ccggctgctg ggttgtggcg tggcgctgct gggggtgctg
     2821 ttgctggtcg ggctgggcct gctggccatc tacttcctgc tcggtctcca ccgtgacagc
     2881 ggcatcaccg agctggcccc cgacgacgcg gcggccgtcg tcgtcattcg ccccggcctg
     2941 ctgcagctca atcagctgcg cgacaccgac cggctgatgg gatcggccgc ggcgctggcc
     3001 ccactgatcg ccgtccccgg cctgcccgat ttcgccttca gcgtctatga cgactatggc
     3061 gaaatgctgc aaaacgccaa catcaacccc agcgaagacg tgctgccgtg gatcggccgc
     3121 gaggtggccc tggccgcgac cgacgcggac acaaatgccg tcgttgtcgc cgccgccgtg
     3181 cgcaacgaga ccagggccaa cgccttcctg gccgatctgc gcgaacagct ggaagatgaa
     3241 ggggttgagt tcgacgagag caaccacggc ggcgtgacta tcaccgagat cgtcggcccc
     3301 gatttctaca cgccgctggc ctacgccatt gccgacaacc ggctggtgct ggcctccagc
     3361 cgcgaagcgc tggaagacgc gctcgaccgg gccaacggcc gcgggcgaac actgtccgac
     3421 aatgacgcct accatgacgc gatggctgcc cagccccgca accggctcgg cacggtctac
     3481 atcgacccgg cggcgctggg caccgcgccc gaattcgaag ccctgcgctg ggtcgccggc
     3541 tccgcgtcgc taatcggcaa aggcctgcgc ttcggctacc ggctagggtt cgaccgtaac
     3601 gcgctcgatg gcgcccaacg tgcgtggatg gaacaaggcg gtatcgacaa ccggatggcc
     3661 gatcggatcc ctgacgacac gatgttctac ctggccgggg gatcgctggc cgaaacgctc
     3721 gaaaacgtcg cctcccaggc gcctgatttt gaagaggcac tgggcgaaat ccagcaggac
     3781 ggcgagttga gcgggctgta tgacctgctg gagatgatga ccggcgagtt tgcgctggcc
     3841 gtctcgcgcg acgacacggg gctgttggcc gagatgaccg gcgaaccttt cggcctgctc
     3901 atcgtctcac aggccgacga cggcgacacg gcacacgaag cgctggacgc cgtcttcgac
     3961 gacatcgcgc gccaggccgg cgctaattat gagacgaacg agatcggcaa tgcctctgtc
     4021 ggctttctcg atgccccatt catcggccct ttcctcggct atggcgtcga cggcaaggcg
     4081 gtcatcctgg gcacctcgga agacctgatt gagaaagcgc tggagccgag cgacgctctg
     4141 gccgacgcgc cccgcttcaa ggacaccatg agcgccctgc ccggcaacgg attgctctat
     4201 ttctacctcg acgccgacgc gattgaccgg ctgatcaatg tattgggaat tgtgtcggaa
     4261 acgtctgaag attacacgcg ccgcatcgaa gcgattgggc tggctgtgga gccgctggat
     4321 cgcggcggcg tgatgaacgc cgagctgttt ttcctgacgg agcggccgcg ccgctgagcg
     4381 cgacctgttc agaattgagg cttaaatctg tggtttttag gagaataaac aatggcaacc
     4441 atcatctgcg aaaactgtgg cacggaaaac cctgacaacg tcaagttctg caaacagtgc
     4501 ggcaacaagc tgccgatcac ggtgccggcc gcccccgcgc aagccccttc ccctgtgtcg
     4561 gggggccaat cggctgcaag cggcgtccaa atggaacccc gctacaaggc gctgcgcggc
     4621 attgcgtcgc ttctgcgaac aatagggttc gtcctggccg tcattaccat tggtggtggc
     4681 gctatcagtt tcttgaccct ggcaaatgat tcgctgctgg tcgccttcgg ttcgctgatc
     4741 ctttcggtca tctccgggct tctcacttat ctgttctttc tcgtgatgag cgaactggtc
     4801 tcggtgcaac tggacatcga ggaaaacacc cgccggacgg cctactttct ggaacagcga
     4861 tccagataag tggggcgaga acggcacgtc gggcaggata accgaaaaat gattcggccg
     4921 cgccggaaat catcacgcgc ggtcagggag aaagatacat gaattcactc gtcgatcgat
     4981 tcaagtccgg cgcgggcaaa gccgcattcg aggccgacaa actgcgccgc gtgacaagcg
     5041 cccaatcggc catcaaaccg ctgcgcggag aagccgaccg gctctatttc gagatgggca
     5101 agctggccta tcttctctat acacagggcg gggtctcgca gcctgaattg caaaccgtgg
     5161 gcgagcgcct ggccgcggtg caggcccaaa tcgccggcgc cgaggccgag gtcgaacgga
     5221 ttcgggccga ggaatatgtc gagccgctgc cgtccgcggt ctcccaaacg gggcttattt
     5281 gcccgaacgg ccacggtacg ctgatgggcg gcgcttcctt ctgccagaca tgcggcgcgg
     5341 ccggggtgtc gcccgcgccc atgccccagg ccaaagcgac gatgccctgt cgccggtgcg
     5401 gcaacgcgct ggacctcgac gcgcgcttct gcccatcctg cggcacgccc gtcgaacgat
     5461 gcgtcaactg tggcgaggcg ctcccggccg acgcgctgtt ttgcgccgag tgcggcacgc
     5521 cggttgccgt cgcagccccg ccccgaacca cgccgcccgc ttccgcgccg ccggcttccg
     5581 cgccattcgc gccggagtgg ctgcccctgt cggccgagga agacacagac accaagtcga
     5641 acacatgaat atggcccaac aaccctgccc caactgcggc tttatcaaca ccggcaacgg
     5701 ccccttctgc ggcaattgtg gccgttcact ggccgaatcg gccgccaggg acgaatcaac
     5761 gaccggcctc tgcccccatt gcggggcgga aaacccgacc ggggcggtgt tttgcggttc
     5821 atgtggcttg tcgccggccg tagaccagcc gccggtcgag ccacaccggc cggccaggcg
     5881 ctacctgttg ccgctggtcc tgctcgccgg gctaatcttt ctgggcgcgg ccgcggcgct
     5941 ctttttgctg ccccggctcg gccaggatcg cgcgtcggca gcactccccg acaaccaatc
     6001 atcgacggcc gaggggtcgt cggtcgccgg cggcgaggaa ggtgctacgg acttggcccc
     6061 atcagatgag gccccatcag atgaggccgc atcagatgag gccgcatccg tggccgaggt
     6121 ctcttctgtc atcaccacga ccacaagcgc gcccaccgtc acccccttcc ccaccggcac
     6181 gctgcggcca accccgacgc ccttgccgac catcacatcg caaccgccca cgccgctgcc
     6241 gccgccgaca tccgtacccc caaccgccat cccgttgccg acgaccgtcc ccccgactgc
     6301 cccgccgtcg gtcgcctgcc aatcgtcacc cggcgaccgt tggggcccga cgctatgggc
     6361 gcgctaccag aacgaactgg gctgcgccac caccggcgaa atccatccca attccgccta
     6421 ccagtattat caacgcggca tgatggtctg gcgagaagtg cccgatctgg tctacgtgct
     6481 ctacaacgac ggctcatttg atgtcttctc ttctcctggg ccggacggct acttccattc
     6541 ggactggctc aagggcagct ttggctatct gtggaataac aatgccacgg tgcgcgatcg
     6601 ggtcggcgaa ccgcaggcgg ccgaattcaa caccactaat ttcgccgtcc aggatttcgc
     6661 cggcgggacg atcatctatt tcctcgagaa tgacgcccgc aattacgtcc tgttcaacaa
     6721 cagcggcacg tggatttcgg cgcagcaatg agaccggccg cgccccatat caggaggcaa
     6781 tccaagcgat gagcgaacct gttgtttgtc cctattgcga ggcgaccaac ggaccaaatg
     6841 cgcaattctg tggccgttgt ggccggtcat tggccattcc cccgccacca cccaaaccat
     6901 caccaccaaa gacagttgat ccgccggtca gaccggccga gacaagtcct tgcctcaatt
     6961 gcggtcggcc caacccgatc ggcacgcgct tctgcctcca ctgcggcgta gaccttttgc
     7021 aatggaagcc ggcccgacgt tcgcgacgcg gatgctggct ggtgctgctg atcctgcttg
     7081 tgctggggcc ggctatagga gcgatcttcc tgctgccgat gttaggcatc gagttgccgg
     7141 agcctgtggc cggttgggtg gggggtggcg ccgagccatc acccactcgt cgagatgagg
     7201 cggacacacc cacggccgaa cctgaacaaa ccatcgcccc ttccgccacc agccggggca
     7261 gtgtcatcgc cgatttgccc acggtcaccc ccttccccac cggcacgccg cggccaacac
     7321 tgactcccct gccgcccggc atgaccgcgt ccacggacgc tccattgcaa cctggggcag
     7381 caccggcaac acccatccca cccacgccta ccctgcccgc cggtgccttc acgctggccg
     7441 gcgcgtcggg cgtggtcgcc atcaatcagc cctgggaaca ggacggcgta accctcacgg
     7501 cgcgaactat cgatatcagg agccaggagg atggagatgc ggccgcggcg cgggtctggt
     7561 tccgattaat caacaaaacc ggcgagcgca tcctgataga gatcgactgg aataatatcc
     7621 atctcgaaga cagcttcggc acgcgctatg tggactattc cggtggcggc acaacctcgg
     7681 aatggatcga gccgggcgca aactttgatt tcgaccgcca ttatacgacc atcgtcgaac
     7741 agcccagccg cgtgcccggc aatgccgctt tcgtccaggt tgtcgtcgat tccttctcac
     7801 gcgtgcagaa cgcgcgctgg caattcgaca tcaaccccgc actcacgccc atctccccgc
     7861 cgccggccgg cgagagcaaa gccgttggcg aagtctggga aaagaatggc ctgaccctcc
     7921 agttggccga tttgcgtatc catgatgatc agaatggcga tgccgccgcg cgggcctggt
     7981 tcgcgctgac caatgccacc aaccaggaaa ttctggtcga tatcgacttc ggtcgtatct
     8041 atctgctcga cagcttcggc cgccgctttg gcgattggga tggcggcggc ctcatgaccc
     8101 aaactattgg ccccggacag accatcgaat tcgaccgcag ctacagcgag atgagcgggc
     8161 ggtttagccg gatcacacgt ggcgcgacgt ttgtcaccct ggtggtagac aataccgccg
     8221 gccagggtac tgccctgtgg acgttcgaga tcgacagccg cctgtcgggc agcgcgccgg
     8281 tcgccccgct ggccatcaat cagccctggg agcaggacgg catctcgctg acagcgagag
     8341 ccatcgagat aagaggccag gacgacggcg acgcggccgc ggcacgggtc tggtatcgat
     8401 tagtcaacaa gaccggccaa tggttgctgg tcgacataga ctggagcacc ctccatctgg
     8461 aagacagctt cggcacggtt tacggtgatt actatggcca ggaattgacc tcattctgga
     8521 tccggccggg ggcgattcat gatttcgacc gctactactc agccatcgtc gaacaggaga
     8581 gccgtattcc cgccgacacc aggagcgtca ctatcatcgt cgacgccttt tcgcgcatca
     8641 aaaacgcgcg ctggcagcga accatggccc ccgagctagt agcccaggac ccgccggatg
     8701 atagccaggc cctggcgatc ggtgacggct gggaacagga cgggttgatg ctgcggctga
     8761 ccgaccttga agtgcgctcg gagagcgacg gtgccgccgc cgccttccgg gcctggtatg
     8821 aactggtcaa caccaccagc cggccaatcc tggtggagat cgattacggc tatctgagcg
     8881 tgacggacag tttcggtggc cgtttcggcg actgggatgg cggcggcctg accacccgat
     8941 ggatcggtcc gggcgaaagc ctggagttcg atcgtcatta cagcgagatg gccggacaag
     9001 ccggccgaat cgctcgcggg tcggcgttcg tcctcgttcg gttcaataac gaagccggca
     9061 tcccgtccgg aatatggcga atggacatta cccggtagaa agaacagcaa ggaaacctcc
     9121 atgagcctga atgcctgccc ccactgtggc ttcaccaaca cccccggcgc cgccttctgc
     9181 ggcaggtgtg ggcaaaagct ggcgtcgctc ccgcccgtcg agcaggatta ctgtccctat
     9241 tgcgagtcgc ccaacccgcc gggcacgccc tactgcggcc attgcggcca ttcgctgatt
     9301 ggcccaaccg tcacaacggc gacgattccc gctggcgagc gggtctgtcc catatgcgaa
     9361 accgccaatg ccgccgatgc tgccttctgc ggcaactgcg gccacgcctt cattggcggc
     9421 cacgccctca ctggcgacca cgccctcact ggcggccacg ctttcactgg cgggaaggac
     9481 gactacccct atactgtccc cgaatcccca ctcgcgtcac gaagccggtg gctctggttg
     9541 ctggtgggtt tatcggccct ggtgctggtc tgcggcggtt cggccatctt gctgtcgatg
     9601 aacggcggcc ggctgccggc ggcgattgcc ggcttgctaa ccgatcccac ggccacgccg
     9661 cccaacccaa cgccggaatc ggcggagcct acacccgaaa cgaccgacac gccgcagcct
     9721 tcgcccgtgc ccaccttgac cgcagtaccg cccacagccg tcgtcctgcc cacgctaacc
     9781 ccattgccca ccccagtgcc caccccagtg cccacctcag cgcccacgaa cgcaccgtcc
     9841 ccagccgtgc cgcccgcacc cattcgcggt tccgacgaga tcgtcttcca gtcgaaccgc
     9901 gacggcgatt tcgagatcta tatcatgaac accgacggca caaaccagcg ccagctgacc
     9961 atcaacacga tcgacgaccg ctttccgcgc gtctcgcccg acggccggcg cattgtctat
    10021 cagtcgggac gcggcgcgaa cgccgaaatc tatgtcatga atcgtgacgg gagcagccag
    10081 cagcgtctca cctttaacga atctctcgac gacctgcccg cctggtcccc cgatggccgg
    10141 caaatcatct tcgttacctg gatcagccgg accgattcag agctgttcgt gatggatgcg
    10201 gatggctcca acctgcgcca aatcaccgat acatcgaccg atgaggggca tgtcagttgg
    10261 tcagtcaacg ggcagctcgt cttcaacggg actgaggcgg gcaccagtaa ctaccagctc
    10321 tacctctcgg acgccgatgg cggcaaccgg cagcgcctga ccaacagccg catcgacgag
    10381 tggtcgcccg aatggtcgcc ggatgggtcg caaatcctct ttttgtcgga gcggggcagc
    10441 agcgttaact caggcatcta catcatggac gcggccaccg gcgaagcgcg gccgatctac
    10501 aaccatgagg attcccgtat cctggaatgg ggcgcggtct ggtcggccga cggccggcag
    10561 atcgtcttcg tgatggacca gctcgacgac acagccaaca tctacatcat gagcgccgac
    10621 ggcggctcgc cgcagctggt gaccgagcgt ggcggctacc caacctgggc cacgggatca
    10681 agatagcgtt cggcgcgaat atcgcgccgg acagggacat gcacctatgg ctgaacgaat
    10741 caaatgcccc cattgcggct tcgacaacgc gagcaaggcc cggttctgcg gcaattgcgc
    10801 tcgcccgttg acccaccaat tctcgccacc gcccgcggcc gatgccgatg ctgtctattg
    10861 tccctattgc gagtcgccca acccgccggg cgcgcccttc tgcggcagct gcggccgcca
    10921 actgacggct tcccagcccg tgccggcctc cgccgcctgc ccccattgcg ggcaggccaa
    10981 tccggtcggg gccactttct gcggcagctg cggccgctcg ctgacctacg acgaggaaga
    11041 caagcgatca tcgcgcggcc ggctcatcgg tctcatggcg atagctctcc tcgtcttcct
    11101 cggagctgtg gcgctcttcg cgctgcccgc gctgggcgtc cccctgcccg gtgctctggc
    11161 cggattaacg ggcgcggagc caacccacac gccccgtccc cgaccggccg caacggagat
    11221 ggaggccgca acggccgaac cgatcccccc cacggcagcc cctgccgcca cggcagcccc
    11281 tgccgccacg gcactccctg ccgccacggc agcctccctg cccaccctca ctccctttcc
    11341 cacaccaacg ccgcgccccc tgcccaccgc caccccaacg cccatcgaca ccgccacccc
    11401 ggacgccggg ccggaacggc tcgtcctggg ccagaccggc cggggaacgc ccatcgaagc
    11461 tgtccgcttc ggcaacgggc cgcaaacggt gatgttcatc ggcgggctgt cggccggctt
    11521 cgcgcccagc accgtggcgc tggccgaggc cgccgtcgat catttcagcc gcaacccgac
    11581 cctgatcccg gagaatctga ctgtctttat cgtcctgtcg gcctcgccgg actcgcccgt
    11641 cgcccccggc gattacaggg ggagactgaa cgccaatggc gtcgacgtca atcgcaactg
    11701 ggggtgcaat tgggcggccg acacgcgttg gggcggcgaa accaaacgcg gcagcggcgg
    11761 cacatccgcc ttctccgaag ccgagagccg cattctgcgc gatttcatca aggagaaaga
    11821 cgcggcggcg gtgattttct ggcaggcgcg cgccgagggc ggcctggtct cgcccggcgg
    11881 gtgcggcgaa cgggtcgagg tgtcggccgc gctggccggt acctatggcc tgaccgccgg
    11941 ctatcgggtg gaaaatttcg aggacctgac tcgacagatc ctcaacggcg acgccagtaa
    12001 ttaccttgac tccgtcggcg tcccggccgc ctccgtgctg ctgcccaact attcatcctc
    12061 gatcgactgg cagaacaacc ttgacgggat catggccgtc ctgaattcct atgccgacta
    12121 gaagatggat tcgtgcggtg ctgctgttgt tgccgattct cctgctgttc ctgcaggcgg
    12181 gctgcgcgga gatcgagtgg tcggccgtca cctttccgcc ggttcccacc gacgtgaccg
    12241 gcccggcaaa cactttgcta ttgcgcgaaa gccggtcacc ggccgaagag ccgctgcggc
    12301 gagtgacgcc gaccgagccg gccacgccgt cccctgccgc ggattcgccc acgatgatca
    12361 gcgcaatcga aggtctgttg ccggccgcaa cccggcctac ggcaatagcc gatgggaaag
    12421 tcgagccatc ccccctgccc ccgccatctc ccgtgcccac ggttccgccc gtgcccacgg
    12481 ttccgcccgt gcccacggtt ccgcccgtgc caaaagccac cccgatcccc ggcccaacca
    12541 ccgagccgac ggccggtcct atcggcgact ccgccggcgg ccgacccatc accggctacc
    12601 gcttcggaca tggccccacg ccaatcgtcc ttgtcggcgg catgcacggc ggatacgaat
    12661 ggaactccat cctcctggcg caaacatttt tcgcctactt cacggcgcac gagacggcga
    12721 tcccggcctc catctcgctc tacatcatcc ccaacgccaa ccccgacggc ctggccgtgg
    12781 cgaccggcaa ggaaacggcc gacctccagc tccgaccgac cgacatcatc acccccaccc
    12841 tggccggccg cttcaacgcc aacggggttg acctcaatcg caactggaat tgtcagtggg
    12901 ccgagcaggg tgtctggcgc gaccagaccg tctcggccgg aacccatccc ttcagcgagc
    12961 cggagacggc gagcctgagc cggtacttcc tggaactgca accggcgctg gtcatctttc
    13021 tgcacagcac ggccggcggc gtttttccct cgggctgccc cgaaatggat ccggagagcg
    13081 cggcggcggc ggtagtctat gggctggcgg cgggctatcc aatctatcca tcgttcaatt
    13141 attacaaggt caccggagac gccggcgatt ggctctcgac acaagacatc ccttcgttca
    13201 cggtggagct tacgacaaag gacgacatcg actgggagca gaacctggcc gggcttcagg
    13261 cggttctgga gcagttccac accccggaag gggtggaact ggaagatgga caaatttatg
    13321 agtaagaatc agatcatctg cccaaactgc aagttcccca acacggtcac agccaaattc
    13381 tgcggccgtt gcggccacgg attggcggcc gccgcgccgg tcgaaccgcc cccgccgccg
    13441 accgatagcg tctattgccc ctattgcgaa atgccgaacc ccgcgggcgc ccccttttgc
    13501 ggcaattgcg gtcgagcatt ggcagccgaa ccgcccgcgc cgccagccgg gggcatccat
    13561 tgtccccatt gcgaagcacc aaacccggcc ggcgccacct tttgcggccg ttgcggccgg
    13621 tcattgggaa cggcaatcca tagcgatgag gccgaaccgt ccggccgcgg ccgttggctc
    13681 ccgctgctcc tgttggccgc ggggatggtc tttgtcctgg ccgccggcgc gttcttcatt
    13741 gtgcccctgt ttcagtcgcg cgccactccc ccggccaatg gcgtcgccaa tgagggctcc
    13801 accgccacag cgaacacagg agccgtggcc gagctggaga cggagaccgc aacgccgacg
    13861 gccgtcccgt cgatcacccc cagcccaacc gcaaccgcca ccccctggca aaccgtgacg
    13921 ccggagccga ccattacgcc gaccgccacg ccaatcgcca caccgaccga taccccgccg
    13981 tcaatcatgc ccctgaccgg caacggcctg atgcagcttt cctttggttc cgagcgcgac
    14041 tacaccccct cgctgtcgcc cgaccaaagc cgcctgatct tttcatccga gcttgggtcg
    14101 agctggcagc tcgtggaagg ggacgtaaac gggtccggcc aggggcttca gttgaccttc
    14161 ggcgcgggca ataatcaagc gccccatttt tcgcccgatg gccggacgct gctggtttcc
    14221 tccgaccgtg acggcgacca tgatatttat ctgctcgata gcggcagcgg cgcggagatc
    14281 gcccaactga ccgacatccc cggcgacgaa tattttcccc gctggctggc cgacgggtcg
    14341 ggcttcgttt tctcatggat ggccaacgac gtcgaggcga tctacttgca gcggctggac
    14401 ggcgcacaga cggagctggt gcgctccacg accttcgacg ggttcgcctg gccttcgccc
    14461 gacggccggc aggtggcctt ctactccggc cgcgacggcg attacgagat ctacctcatg
    14521 gacatcgacg gtggcaaccc gcgccggcta accgtcagcg gcggccgcga cgcctcaccc
    14581 acctggtcgc ccgacggccg gtggatcgcc ttcgagagcg cccgcaacgg cgattacgac
    14641 ctctatgtca tgcgacccga cggcagcgac ttgcggcaac tcacatccgg ctccgtcaat
    14701 gactggttcc cgaccttctc atccgacggg caatggttgt tgttccagtc ggaccgggcc
    14761 ggggaaatgg atatattccg gatgcccttc gcgccctagg gctttcgccg ccctctcagg
    14821 aggagcgcgc caaggctgag caggaagacg gccccggaga cggccatgac accgaacaaa
    14881 atcggccgcg ccgcgtttcc gcctataggt ggctcagttt catcaactgt gccatcggca
    14941 acgatcgggg ctaccccatc ggccgcttct gccgatggct gggcaacgag cgtcagcgtg
    15001 cgtgtgtcgc tgcggtcgcc ggccaccgct cgcgccgtca cgcgcaggcg ccccggctcc
    15061 gacgacacgt agacgggcga caactcggcc gcttcccccg gccccagatc gggcacggcg
    15121 acctcggcta cggtttggcc gtcgatttcc agcatcagcc gcgccccgct gaacggcctg
    15181 ccgccggcgt tgatgacccg tatgggtatc tccgtcgctt cgccgaccac gaccgcccat
    15241 tccgccggcg cgtcgaccac cgtcagggcg gcatccggcg cggccggcaa cggaacctgg
    15301 gccacgacgc ggccgagcag atcggcgttc tcttcggcct gcagcaccac atcatagatc
    15361 gccgctgcgt cggccgcctc ggggtcggcc agccaggcat cggccgccat gagcgtgaac
    15421 agcgcatcca ggcgagcgaa cgtgatggcc gtgcccagag ccatcgcacc ttcttcggct
    15481 tcggcggttg ccccttcggc cgtcgcgccc aggcgggcca tcaccggcat ggcttcctcg
    15541 tcgagagcgc gggtcgcggc cagcaactca tcggttcgcg cgcggaccgc atcgggatcg
    15601 gccgcattca gcgcgtcgga caaggcagcg atggcggcgc ggtagccggc cagcgccgcg
    15661 tcgaattcag gcatggttga ttgggccggc ggggccagcc gcccgacata atgcttcccc
    15721 ccatccttct cggtgaacag ccggcggaag aaatcaccca ggtccaatgg cacgggtacc
    15781 tcgcaaacat acacagggcg atccggctgg aaggcgaact cgcccgaaag cagggccgcg
    15841 tcgcgcgtac aggtcattgc gccggaaaga acggaggtgg ctgccgtgtc gatcaaaacc
    15901 tgttggaggc gcgtctggat gctgaagatg agaccgatgg ggttcttcgt acccaacaga
    15961 gcgagatcga cgatgtcctt gagaagctcg ttcacatcgt gcccccgacc cagcgagttg
    16021 gtgtaaacgt catgggccag ctcgcgcatg atcgccgatt gttcggttag tccatcaagg
    16081 ccgatctgcg ccgattcagt ccgaccgccc accgtccaga tcgggttggc ctcgccggtc
    16141 acgctgttca cgccctggtc gacgaccggt tgaacggcgc cggtgaattt ggggatcagt
    16201 tcgcggaatg ccagcgcccg aacgccgtcg tcctgtgccc gctccaggaa ttcttccgcc
    16261 attgccgacg catcgccagc gtcgatcacg ttatctgcat aaacgtccag caaggtgata
    16321 gccaggccaa tactgtcgct cattcccccg cgcaggtctt cctcgtcgat aaaatgcagg
    16381 agcaatttga tgaaatcttc gatgactttc atcaacaaat cggcgatagc cgcttgcagg
    16441 ccacccgccg ccttggccgc cagcaagccg gtgccggtag ccatgccggc cagatcgacg
    16501 gcgacatcgg cctggtcgct ggcgaggacg gtgtaatcat cgagcaaggg gtccagcgcc
    16561 tcctcctgca cgaccagccg ctccagcgcg gcggcctgct cgggcgagac cgacgccggg
    16621 tcggcggcgc gcagcccgtc gatgagcgcg cgggcggccg tctcgtcgaa ggcggcgatt
    16681 gggtccggat agagaacgcc ggccgatgtc tgatagccgg tcgtctccag ctgctcaaag
    16741 acggccgatt tgcggtcgag cagcgcggtg agatcgacgg tcacctctac gtaatccggc
    16801 cgaaagacgg ggccatatcc ggtgatcccc acgctcgtga tggccccggt aaccaaatcc
    16861 atcacttcga tctcctgacc gcggacgtag atcagcgaac tcccgtcggg tgatacatca
    16921 tgccagccaa tcgaccccag ttcgttaggc atttcagtca ggacaacagg ggaagcgctc
    16981 agggattcat aatagattag agacgaggac agggagccgt aatcggagcc atttgtgaag
    17041 agactcgtgc cgttcggcgc atacttcggg tcggaacagc agatcctgtc gtcatagcgg
    17101 cgtggcgaga cgggtgaatc ataaaaagcg atccgggaac cttcatagaa accgacctcg
    17161 acggcgatca cctcatccgc gccaagcgaa atagaataaa ttgcgccggg cgcttcgcag
    17221 tcgacgagga ttgcttcacc cgtttctata ttcatcacgt acaggcaacc tttatggaca
    17281 tcgccccagg ggtcgatatc gacccattcg gaactgctga aggccaatga acggccgtct
    17341 ggcgacaacg ccagaacgtc gaatagattc atatccggag tgtcgatcac ccgttcctgt
    17401 cctgtccgaa agtcataaac catgatggtg ccgggagagg cagtttctgg cgcatccttt
    17461 aatctggcat cgcggataaa atagagcagg ctgccgtcgg gcgaccaggc caggcaacag
    17521 tcggtgtcac tgttcgtgat ctgttgcgga tcgctgccat ccgcgttcac caaccatatg
    17581 ctcccttggg tttcccactt tccaccattt ggctcgatgt aggcgaactg ccccgcggag
    17641 gccgagccta ccggcggagg agtggacgcc ggatcaaggt aggtgatgcc caaatagttg
    17701 ccggcggcgt cgaagtcata agcgacaagc gcttccccca catagccaca ggcatggcaa
    17761 ccatcgacga tctgaaagac gaacgtgaac cgctgaccgc cgccgggcag agccatggcc
    17821 tgcgggctgc ccacatctcc accccatagg atggcctggg ggtattgggc gagcagagcc
    17881 tcataacccg gaatggttcg cagatccggg aagaatgtct cgcgcgtgga aacgaagggc
    17941 gggttgccgt tgagcatcgc gtagctccca ttgtcgtttg cggcgagagg atgaaagatc
    18001 gtgccgagat cgaccacgcc cgactcctga aaatcgctca ggtaatcgaa aaatcccctg
    18061 gattcggcaa accctatcgc cgcggccgat gcgccaaggc cggccataat cccggccggt
    18121 gaacaaccgg cgtcctggcg acaggcattg atatcttcag cggcgctaaa ccccggctgc
    18181 cacaccgctt ccgggccaat ttcggtctgg gcctggacgg aaacagagag aacgagcaag
    18241 cagaagaaga aagccgggat aagcgggcgg attggcgcaa gacgcagcag cttcactcga
    18301 tttgtcctaa tcattcatcc ctctcacgga taggccacgg gcaacggtgt ggcctcgatt
    18361 ttcggaatac cctccaccaa caccacccaa tccccatccg ggctatgggg cggcgcgccc
    18421 agcggcagcc cctcggccga tgcgacgacg ctaacggcct cccctgcaaa ctcgcccgtc
    18481 cgcgggtcat accagcgcag ccggtattcc cgaccggcgg ccgcctgcag cacgccgctc
    18541 gggttggccg cgggcagata gacggcgaaa atctcgtcat gataggcgaa cacctccccg
    18601 ccgccgaacg cttccgattc accgctcagc aacgcatcgg ccggctccat ggcccaaaac
    18661 ggcaggttgt cctccataaa ccggcgcgca taccacatgg tgcgccacat ggcttcgcgc
    18721 gtgcgaaaat cctccaggtt cacatcgccg cccggcggca ggtcatagta accggcgtac
    18781 cattctatat tcccgccact gaaatagaca tcatagagaa ccgtcttgcg caattcgccg
    18841 gcgttgtcgg ggcctagacc aaccccggcc gggttgttct cgtccatatc gaccacccac
    18901 ggccgcccgg cactacgcga taactcccgc catcgctcga cgatctgccc ggcctcgtcg
    18961 atccggtagt gaatggctgt ggtcgggaat cggctgtcgc ccaacagttg ttgatattgg
    19021 ccgaacgagt cgaaaggatt gtgcacggcg atggggtggc ccgcccaatc ataggcgcga
    19081 atcgtatcgg cgaattcgcc gatctccgcc gccgaaaaga cgttctcctc gctcagattc
    19141 cacttcagcg ccaacagata accgaaccgg gccaccatct cgcgatagaa cagcttgcgt
    19201 tccacgccca gcgcgccgcc gtcgagccag cggcgattcg cttgctccgt ctcgttcagc
    19261 accacatgca gggcgatgcc cattcgctgc gcgtgttgca ggacggtatc ccattgggcc
    19321 agcttgccca cgtcgtaatg ggtgttggcg ccgggcgacc cgtcgggcga aaggaacggg
    19381 tagctatcct gtccgtcgcc gcccagattc atgggcagaa agtagatgga attgacgtgc
    19441 tgttcgctca gatagttgag cgcgccgatg atgccgcggc cgtccatgtc gccattgggt
    19501 ccggcaaaat cgggatcgcc ctcgcgccag tcaccaacgt gaggcgcgta ggtatggaga
    19561 aagttgggga tgatcccgcc ctgatcgatt gtgttatcga aaccggcata gcccaggaag
    19621 ttctccgggc tatctgtgcc gcccttgatc cagtatgggc cgtcgcggaa cttgaggtaa
    19681 tgttggccgg tatattccag ccgcccccat ttcaggaacc ccggcgcctc cgggtcgcgg
    19741 tcggtcacca tgaactcgcc ggtgcggccg tcgaacggca gcggcgcgcc cgccaacatg
    19801 tccgcatcca cagcgacaga ttcgccggca cgaaacgaga cacaatagcg ccacgccccg
    19861 gcttcgtcgg gcgaaaagcg gacctgccac acatcaccgg ccccgttgcc gcggccgtcg
    19921 ccggcaaaat agccgggcac atcgtaggcc tgcccacttg gccccagaaa gcgaacctgc
    19981 aggcgataat caaggaaagg gttgggcgac tcgtcggcct catcgacaac cgggccacgc
    20041 acagccaacg tcagcggata ccattgcttc atctctccgg tgaccgtcgc ggcctccccg
    20101 ccccgctcct cgttgcaacc caaatcggcg tagcgcctca taatcaccgg cgcgaatgag
    20161 tatgccgccc ggccggccac ttcctcggtc tcactcagga tggttaccgg cataccaacc
    20221 ccggtggcaa ccaccggtgg cggcaaggga cgggttggcg cggcgaccgg cgagggagcc
    20281 ggcaaggaat cggccgcggg cgaaaactct cccggccagt tcgcccccaa ccccgcccca
    20341 caaccggcgg ccgacaacaa acccatcatc aaaagaaagg caagaagggg aattgagcgg
    20401 cgagccatcc cggcgaaacg gctatccatt cctgagacgt ggaacatagc ctaacgggtc
    20461 accgtgaatg tgacagtcaa cgggagcgtg catggccctt cgccattatc ccgatcgcag
    20521 ggctgggcct ctatagtgtg ttgcccgccg acatatcggg cccaattcca gttgccctgc
    20581 ggatcgccgt tgatatcgcc ggccatataa tagggcgcgg tgttttccag ggatcggcca
    20641 ttgatatcga tcggcttccc gtcgaacatg aatataacgc tggccacatc cgcgccatcg
    20701 acgatagcct cgacgttgag atagcacgcc ccactcgccc cgcaaccgct ctgccgcaga
    20761 ctgattgtgt caccgtcttc gatggcgccg atggcgcgcc ccgtcgttgg gttgattaac
    20821 tgaaagctgg tgatgcgcga cttctcctgg ggttcagatc cctgtggctc gacgacagcg
    20881 acgttgccgc ctgggcctga ttggctgccg ccgctgttgg cgttgttggg gtcggtgtta
    20941 tgggtcttca cctcctggcc gtcgctttgc ccgtcgccgt cgctgtcggc gctgcgcggg
    21001 tcggtgctgt aattttgcgc ctcctgaccg tcgctcagcc cgtcgccgtc gctgtcggca
    21061 ttgttggggt cggtgctgta gttttgcacc tcctggccgt cgctcagccc gtcgccgtcg
    21121 ctgtcggcgc tgcgcg
//
LOCUS       DAOMQG010000020        20888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1102_length_20888_cov_10.102194, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000020 DAOMQG010000000
VERSION     DAOMQG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 20888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20888
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1102_length_20888_cov_10.102194"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PLC06_02465"
     CDS             <1..1016
                     /locus_tag="PLC06_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO89338.1"
                     /translation="FFDRLPGPASAGSRMQLAQDAFDLTGDYWLTGAGLNSFDGLYSQ
                     YIQVIPFHAVIHSHNLFLNIIIEEGILGIASIGAILALSFWWLSDPKLSGYRRSIHGF
                     SLLAGATFAMLVVLCLHGLVDDVLFGSRGILLLWFPAGITAMLFPRRQGLREWVKAAE
                     SPRLIFLGVVTVITALLLVTFRSSLLSSWYATLGAIEMDRVELANYPSGEWSDGSEAV
                     DLGVAVSQFNRALLYDQDNRTAWHRLGLVAMLDRDFETAADSLYHAYLLDSQHRGIRK
                     SLAYARLWAGDLEQAMPLLQEVPDVELELANYVEWWKSQGRPDLSERAEAARNILTNL
                     NSH"
     gene            1428..3737
                     /locus_tag="PLC06_02470"
     CDS             1428..3737
                     /locus_tag="PLC06_02470"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRO89339.1"
                     /translation="MNGNIHAFWVDDLSDKAGVLKHSAVPESSFVSYEAWSAPESLEF
                     GAVSPAVAVNAAGLHVAYIRRVETLDRPAGVYYRRLSVGNGTWSGERLIYASRYLRGL
                     NAETANVSIAAAETGRIVLAWDDPGREQVFVSTSSDDGANWTAPIEIDRRAVTDLPAS
                     AGPRDIMAGVSSGGTIVTWRADHQSFQSCVHYYRSLSDGSESWSLPQAIPGLADCLTT
                     VNIVSSNGNLFLLGMVEPLDSVAGGAKPATYLLAWDGARWSLPRGQEALAGFVNPETN
                     QPIDLRCFDVATLADKLNLVGCDHGVGGDIWWTYRTFGDITVWFPPPAVWKGPTAVAA
                     VATPVSDLGLARDSAGRNHLTWTEQGGRQIFHAYHDDAGWSTARSIIALDTGTIQSLD
                     MTGNGTRLFIVFRESDGLHFAQAGVERPAEWTTPVPLAAGQTEAVEPDILVSQSGALF
                     VAYTVALNEPRGAYLTRSTDLGANWSEPIQLFSGAAADWPAVGYVNLVETTDGRLHAV
                     VAERALPPDDTILRLAYSRSEDGGMTWSATTPIVSTPMRWSSLIGYGERVLHMLWAEP
                     TNDRLLLWHIQSVDGGNSWDEATQVGALDGTDLPAVTMDPTGQLHLLGMEDGRLLAWT
                     FDGAKWTAAESATLNLADGGLLAAEVDSAGNLVAAYGLLVPGSSPEEATGGLFVMTRP
                     LDLPAVALPTPPPPPATMAPEPTVVLTATPEPTPTVIVPTAPDSNLLSSVPGAGSRAG
                     QLAIAVIPAALVVLIASIIGLRVMRRGGR"
     gene            3761..5155
                     /locus_tag="PLC06_02475"
     CDS             3761..5155
                     /locus_tag="PLC06_02475"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRO89340.1"
                     /translation="MALALLLTVLFFPLAGYSIAQNQGEWSKPELIYETEAAINYPYV
                     VGDGTGVTHLFWREFDEDESDSDSGLQAIIYSNDIGGRWNPGRDIIVMKSVVAPAAAV
                     DANGLIHLLWQSTNNTLYHSLATTESAHSAQGWAQPEPIAATNSSAHIQTGIDNVMHV
                     VFPGIDTTGVYYIRYDNETHGWSFPVNISAPASSNTSSDFTRLAIGSDGTLHVVWTEY
                     LLPGGWPPTGIYYAQSTDGGGTWSRATELEGAGSVQANVAVDSRGRVHVAWNRMAGIG
                     GRYHRWSEDGGQSWSPLAEIAPAGQGGTEGPPQLVVDSADALHLLTTFNGCAQYATWV
                     EGVWTSPVCISGREAMASGFIESPTMTVTNGNQLHAVFWDDRKRLWHATMTTNAPFIP
                     SVALIESAPPTIAASPAAVEVTQSVATPIINLSTAPPGDGELLGPAQALLPAVAIAGA
                     LLGFVILIKLLRSR"
     gene            5169..6053
                     /locus_tag="PLC06_02480"
     CDS             5169..6053
                     /locus_tag="PLC06_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89341.1"
                     /translation="MAEQSKRSPLYTSATIDVADVVSRPALIIEPARGHFSLQLDRLW
                     AYRELLYFLVWRDVKVRYKQTALGVAWVVLQPLLTTLIFTVLFGLLLKVPSGDMPYPV
                     FALAGLIPWQYFAGALTRTSSSLVGNAHLITKVYFPRLIIPLTGVLSGLVDSAIGFVL
                     LAALLVVYGIWPTAAILLLPAFLLLAMATALGFGLWLAALNVRYRDIGYLVPFLIQIW
                     LYATPVAYGSTLIPERYRWLLGLNPMTGVVEGFRWALLGQEPPGRLFALSVTISVVVL
                     LTGLVYFRRTERTFADVV"
     gene            6090..7343
                     /locus_tag="PLC06_02485"
     CDS             6090..7343
                     /locus_tag="PLC06_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89342.1"
                     /translation="MSDIAIRVENLSKRYEIGRAGTRHDTLRDALAGLFRREGRGRSE
                     TFWALRDVSFEVKRGEVVGIIGRNGAGKSTLLKVLSQITEPTGGRAEIHGRVGSLLEV
                     GTGFHPELTGRENIYLNGAILGMRREEIARKFDEIVAFSEIEKFLDTPVKRYSSGMYV
                     RLAFAVAAHLEPEILLVDEVLAVGDAAFQKKALGKMGDVVNEGRTVLFVSHNMDAVQR
                     LCNRALLVANGRIITQGDVKTSIREYQREQLTVRNAFAWHDISQAQRTGSGDVKFSAV
                     KLCGGSLPVDAIFTGGPLSVELDIVSKISTSVSSMAITVFTPNGTMLVNADTISQGKT
                     FNLREGRTRVQFVVYALHLSPGTYNLGLWLANHPGIIHDRVPTAIQFEVVEYGQNNQP
                     GVRVDGLVPCSFKVEALDFVPDGDE"
     gene            7405..8226
                     /locus_tag="PLC06_02490"
     CDS             7405..8226
                     /locus_tag="PLC06_02490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89343.1"
                     /translation="MQILKSGSRNRLVFFLAIITLSFALLFFITRALDERLALMASVA
                     WATLLIIYLMYRWRSIPPIYDLQLIELRHLIELQSLMGGAFLPFSVWAMAPNDLFQLV
                     STIQIQRYGTIVECGSGVSTITIGRLLRQLGIGHLYSLEEDKAWYEMMVATLAAEGLT
                     GVVTLIHAPLELYQGADAEWYALEQVRQVRAGAGHIDLLLVDGPKSVSTYSRYPALPV
                     FVAQLDGSSLIVLDDTRRPGETAVLERWSREFQLRFEDQPGSPRGQAYIRLVKEL"
     gene            8223..9407
                     /locus_tag="PLC06_02495"
     CDS             8223..9407
                     /locus_tag="PLC06_02495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89344.1"
                     /translation="MNHPLVSIIIPTYNRKEYIQQAIDSVIVQTYPHYEIIVIDDGST
                     DGTGEALVARYNDRIIYHRQENQGESVARNWGINVARGEYVGFLDDDDIYLPRMLEVT
                     IEILEQHPDIGFVSVQYYVTNEAGDILYTPLLSNSCDGYITAEEFLLGDSTVPSNSIL
                     RRKVIDQAGYFDTTIRYGEDAEFFRKIFAQFRAYFLAEPLMAMRVGEARQSGRILDPA
                     KAKLRFDDQTAALSRIEDDPVFVPYIPKLCGLINANYAFNLIANGETSKAEPILKHVF
                     TLEPSDWFYKEIMCGLFLQYIEALDDEQGIAAVMKFAQQVFTAVDGTRAFRQEWRRKI
                     IGKFHLNRFFKQAFQEEWAEMRLSYFRAIANDLVCLKNRGIHSFMIKSFIGPRLLRTR
                     VF"
     gene            9441..10583
                     /locus_tag="PLC06_02500"
     CDS             9441..10583
                     /locus_tag="PLC06_02500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89345.1"
                     /translation="MPTISVIIPTYNGARYIRQAIDSVLAQSYSDYEIIVVDDGSTDN
                     TAEILWPYGDRITYLYQSNQKLPTARNNGITASKGKYLAFLDSDDLFLPDKLEVQARY
                     LDERPDVGLVASGWQYIDDDGWILSTSEPQEDRPITLETILFGGLAPVHAVLLRREWF
                     GRVGGFDPQFAYCEDMDLWYRLALAGCPMVWVEAIVCQYRIHTDNMSRSPEIHFNYYR
                     RAMDKAFADPRLPKELLARQAELDANLDLSEASRLVAGGWTEDARERVLRAVSINPRL
                     KDDNGHGLAEIVVGLRSSVWSDGRFAEFVATVMADEIPALDHTMAIVSAKKCFYNAYN
                     KQRVTDIRRAWFDIICRDPRWLVDRGGWSILGQSFVGFKQASGTRE"
     gene            10596..11594
                     /locus_tag="PLC06_02505"
     CDS             10596..11594
                     /locus_tag="PLC06_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89346.1"
                     /translation="MSDLVTVILTTLNSEKYLRKSIDSCLNQTHHHIELLVVDGGSTD
                     ATIEIVESYIKADPRIRLIYQENNAGKLPGAINLGMANARGEYITWTQDDCWYEPDAL
                     ELMLAFLRDHSRVALVYTDYWLVDNLYGQQAIKYTRVSDVDRMLDEDVIQQCFLFRRE
                     VYEAVGPQDIQYFPIHEVPWRLKITQGFRIEPLHIPLQYYHWHVDSLTGRIGGYELQR
                     MMYRAFLIEGHIDEGELRRRLADIDIHDAYAAFVFRGDYRAFRSLFKAAILGNPTHGR
                     NRGLWKLFVTSFLPAREWRRQEMLDAWQAQEAKRQVILVHEFRERFPQRQVAHAND"
     gene            11581..12807
                     /locus_tag="PLC06_02510"
     CDS             11581..12807
                     /locus_tag="PLC06_02510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89347.1"
                     /translation="MPTISVIIPCYNQGRFLKFAIDSVIAQTFEEWEVIIVNDGSTDD
                     TAQVVAGYDDARVRYVYQENRGLSAARNTGLRESHSEYVAFLDADDAWTPSFLQHSLL
                     PMLNDQRPGMVYSRYRFLDDRGNLQSQIGGEALESTRFRRVIRRGGFFPPNCALVRRS
                     AIDKVGCFKSLPTGAEDWDLWLRIAEEFPVVGLEDPLAYYRIYPGSMSTNGLRMQISR
                     FAVLAEHFGPPDGPPGLWPEAKREAYACGYRSCACEFLQQNDPDTAWELLSEGARIWP
                     PLLSEVETYYEFILGDQQKGQRGRADLLDLPAREKDVISRLDDLFVGEADDKVKSQRR
                     VVRARAYLALAMLADQAGDWAAARGYLRKAVRLDPLLCVRPEIMRRFAKLHAGQRFVR
                     RWKAGKPADRTVPRGG"
     gene            12998..14101
                     /locus_tag="PLC06_02515"
     CDS             12998..14101
                     /locus_tag="PLC06_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89348.1"
                     /translation="MKVLYVPRSDADVVTGGDVLYARSLCQALCPLGVELVCVEVDHL
                     EHVVDADAVFLTQIYQIDVAEKVAHWASRRGIPLLISPLYEEGDHLGFRLTVQGEGKW
                     SRISRIIGSQAAEELYVRREAARRTRQDIWQRQRQLLQQAYVVSNTHYELEHLRRWFK
                     LTNLRATIIPLGIDPGKFNLESGGATEFLPAQIQAWRGKYLLQVGLISARKNQLSLLR
                     AMDDSLIPVVLIGRHSPYEPGYVSEVTQLARARGTVAMLEHVDLSTLAALYGQAAAHV
                     LPSWSERPGLVSLEAAACGCNVVTSTAAPIWEYLGQEVDICAPNSSKSIRQAVLNAMN
                     RKSTLSLSNKITSTYTWDRTAGAFRQMLRDIIA"
     gene            14116..15369
                     /locus_tag="PLC06_02520"
     CDS             14116..15369
                     /locus_tag="PLC06_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO89349.1"
                     /translation="MRILFLSNVYPPYSQGGYEQLCQEVAQLLAARGHTLCVLAGRTT
                     GAADVEEDGPVRVHRRLELELEGGLSHTIARLAIGGREASERRNVATVERLIDEFQPD
                     AVLIWGMWNVPRSVPAAVERLLPERVAYYLCDYWPVLPNAYIQRLEAPARRGHADWAK
                     RGITQLLLPAAAAEMPTSLSFKRAACVSYALRDALVHQGTPLDNAQIIHNGIYLSDFS
                     VGSDAPSTNNSLRLIYSGRLSPEKGLHVALEALALLDGSVANRCELLIAGGGDEDYLR
                     HLRTIATRGSVPVTFLGHVGRANIPRLLSESQVLIFPSEWGEPLARAVIEAMAAGLAV
                     IGTTTGGTGEILVEGKTGLTFRPGDPADLARQITWLAADPRLREQLAAAGQALVLREF
                     DIRSTVDQLESLLCQATIQSESPVA"
     gene            15366..16565
                     /locus_tag="PLC06_02525"
     CDS             15366..16565
                     /locus_tag="PLC06_02525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89350.1"
                     /translation="MNLLYLSSWFPFPPTNGSELRINALIRGLAARHDVTLLSFQRRP
                     IDPRGLAQARAILSDVRLVPWREFAPDSRRARLGFLSSQPRSVVDTYSTEMDMLIREV
                     IAMHQYDAVVVSQLAMAAYWPAWGDLPTVLDEVELGSSREQSRLASTPMARVRRALMW
                     AKLQSYVRGLLPHFDAVTVVSQRERDLLRQVAPDYSAVSIVPNGIDLAACASVRAGHD
                     PDTLIFTGAFTYDVNHEAMVWFLDEVYPIIRQNVPQLRLTITGDHAGKPLPSADGVTL
                     TGFVDDVRPLIAGAGAAVVPIRQGGGTRLKILEAMAVGTPVVATSKGAEGLDVTDGEN
                     ILLADTPQDFAEAILRLRQDAALCQNLVNNACLLIRRQYDWVVILPDFIELVERVASR
                     AMTPVQQ"
     gene            16572..17987
                     /locus_tag="PLC06_02530"
     CDS             16572..17987
                     /locus_tag="PLC06_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89351.1"
                     /translation="MMRQIFTPQAIRSSQGARNVWRLALIVAVLLGSAILAYIVPVQR
                     KVLLVPAAMLAIAAAYIFLRRPSLGMMALVFAGLLVPFSIGTGTGTSLNPVVLLVPAL
                     TVLWLAENGLRHHAIRLRRHSSVYLLLVFMAVATLSYIVGLLPWFDIHGAGAAAQIGG
                     LMVFYISAAAFLVSAHTLNERWLARLVYLFIAIGALIVLGRFVLPLGRVLAQFVDGAA
                     MGSVFWIWLVALSAGLALFHDDLPLRWRLVLIAVASLALFLGLFQNREWASGWAPPLV
                     ALLILVWLRFPRWGWIGLFVATVVFILEIDRFWMAVTTDQSWLARQQAWRIVLDTTSV
                     NPVLGLGPSNYYFYVQNATIRGWGGAWNVKFSSHNNWVDLIAQTGILGTAVFVLFTLS
                     LFRVGMRLYTGQANGFPRAYAAACVAGLVATLISGFLGDWFLPFVYNIGLAGLRASIL
                     FWVFMGGLLMLYMEQENSSNP"
     gene            17990..18913
                     /locus_tag="PLC06_02535"
     CDS             17990..18913
                     /locus_tag="PLC06_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89352.1"
                     /translation="MELSVIIVNWNTRDLLAQCLESVYANPPNGEFEIIVVDNGSSDG
                     SVDMVRERFPEVLLIENLENTGFARANNQAIRISAGRYIQLLNSDAMVTPDSLEAMAT
                     FMDFHPQAGVVGPMLINRDGSFQASFAEFPTLWTELCLLTGISRLVIGPYAPSPRPKL
                     YAVPEQVDWVAGAAIMVRRAAIDRVGLMDETYAFYSEETDWCWRMNQWGWQVWYIPLI
                     KVYHIGAASSRKRSHESYIQLYDSKVRFFKTAYGPRAANRLRFVIKVMAFLRLTVRAL
                     MLPITTAFSKSRTMPRIQQDIALIRHMISSD"
     gene            19214..19861
                     /locus_tag="PLC06_02540"
     CDS             19214..19861
                     /locus_tag="PLC06_02540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89353.1"
                     /translation="MPIARSLIGWRAYRRVPPRTKGNLLDVGCGTGEQAAWLRDNLLN
                     WEVEGVEINSHAAQIAESLFNLKVYQGHLPDLNLPPEKYDMVSFWHSLEHTYSPNATL
                     KETFRLLRRGGWVGIEVPNICSWEARQTKEHWYHLAIPVHLYHFSVITLSALLKQNGF
                     TPISVEKVRGRAGLDNWIEHNRANYSWTQGIAVKAGVQTLGRFSPWGIRIYAAKV"
     gene            19845..20822
                     /locus_tag="PLC06_02545"
     CDS             19845..20822
                     /locus_tag="PLC06_02545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89354.1"
                     /translation="MQRKFDAVSNRKPSVDMSILIVNWNAQGLLSRCLASLEGSVGIR
                     IETIVVDNASIDNSVAMIRANFPNVYVIANGENKGFAAANNQAAAQATGRYLLLLNPD
                     TKIPDGSLAELIAYSDTIAQLGAVGPRVINPDGTTQRSCWRGYPGIAAALIDAFYLWK
                     IPGLPFVRNTEISADHLRNVLEVDHILGACMLIPRDVWQEIGPLDEDYFLFLEETDWC
                     RRAKALGKRIVYLPTAEVIHYGQHSMRQQPSRNLPHLYRSYCRFYRHGSGSDGFRMFV
                     LKVVISLAILVRLIMWQIRGFFSHTEKQREQARDMLVGYFQVLRELPSM"
BASE COUNT         4300 a         5492 c         5965 g         5131 t
ORIGIN      
        1 gattcttcga tcgccttcct gggccggcta gtgcggggag tcggatgcaa ctcgcccagg
       61 atgcgttcga tttaaccggt gattattggc tgacaggagc gggattgaat tctttcgatg
      121 gcctttactc ccagtacatc caggtcatac ctttccatgc ggttattcat agccataatc
      181 tatttctaaa tatcataatc gaggaaggta tcctcggtat tgcttccata ggtgcgatcc
      241 tggctttatc gttctggtgg ttatcggatc ccaaactgtc gggttaccgg cgttcaatcc
      301 acgggttctc cctgttggcc ggagcgactt tcgccatgtt ggttgtcctg tgtctgcatg
      361 gacttgtgga tgacgtctta tttggtagcc gcggcatact gcttctgtgg tttccagcgg
      421 gaataacggc catgttattc ccacggcgcc aaggcttgcg ggagtgggtg aaagcggccg
      481 aatcgccgag gctgatattt cttggtgttg ttacggtgat taccgctctg ttacttgtga
      541 ctttccgctc atccctgctc agctcatggt acgcaacact cggagcgatc gagatggatc
      601 gtgtcgagtt ggccaactat ccaagcggcg aatggagtga cggctctgag gcggtcgatt
      661 tgggcgtggc cgtatctcaa tttaatcgcg ctctattgta tgaccaggat aatcgcaccg
      721 cctggcatcg gcttggtctc gtcgccatgc tcgatcgcga ctttgagact gcggccgact
      781 ctctctatca tgcttatttg cttgatagcc aacatcgggg aatccgcaaa tccctggcct
      841 atgcccggct atgggcaggt gatttggaac aagctatgcc gctattacaa gaagttcctg
      901 atgtagaact ggaacttgcc aactatgtgg agtggtggaa aagccaaggt cgtcccgatc
      961 tcagcgagcg tgccgaagca gcacgaaaca tcctgaccaa tctcaattct cattaatgct
     1021 ctatgcgttt caaacatagt ttattcatag cgatattgct cctgatcgtg ttgttgaatt
     1081 gctctgtcgc aaccgggcag atattggccc aggccggggg gaatggttca tcggccacgg
     1141 atgcaatgct gggatctctc gcttttcaat caagcgacga atggtctgcc ccactcaacc
     1201 tttcccgttc aagtgcgaca actaacccgc agttggttat cgcgagcacg gggcgccaac
     1261 acgtgctatg ggaagaccag tttgaggggt ttgtttatgc ggcaggcggg tcggatggct
     1321 ggagtcctcc gcgggcagtc gaaacaccgt ttttcactcg tcgcgtgttc cccgatttac
     1381 aaccggcggc tgcgacacca cgctttatcc cgcttcttgt cgccgatatg aacggcaata
     1441 ttcacgcttt ctgggtcgac gatctcagcg acaaggccgg tgtcctgaaa catagtgctg
     1501 tcccggagag ctctttcgtc tcgtatgagg cctggtctgc gccggagtca ctggaatttg
     1561 gagccgttag tcccgccgtg gccgtcaacg cggctgggct tcacgtggcg tatattcgca
     1621 gggtagagac gcttgaccgg cccgccggcg tgtactatcg acgtctttcg gtcggtaacg
     1681 gcacgtggtc aggcgagcgc ctcatttacg cttcacgata tctgcgcgga ttgaatgcgg
     1741 agactgcgaa tgtatcaatt gcggcggccg aaacggggcg tattgtcctg gcctgggacg
     1801 accctgggcg cgaacaagtc tttgtctcca cctcgagtga tgatggagct aattggaccg
     1861 cacccatcga gatcgaccgg cgcgcggtga ctgacttgcc ggcatccgca ggccccagag
     1921 atatcatggc cggcgtcagc tccggcggca caatcgtcac ttggcgcgcc gatcaccagt
     1981 ctttccagtc atgcgtccat tattatcgct ccctgtctga cggcagtgag agttggtcgc
     2041 tgccgcaagc cattcccggc ttggccgact gccttactac cgtcaacatc gtcagcagta
     2101 acggcaactt gttcctgctc ggcatggtgg agccactcga cagcgttgcc gggggagcga
     2161 aacctgcaac ctatctgttg gcgtgggatg gggcgcgctg gagcctgccg cgagggcaag
     2221 aagcgttggc aggctttgtg aaccccgaga cgaatcagcc tatcgatttg cgctgcttcg
     2281 atgtggcgac ccttgccgac aagctcaacc tcgtcggttg tgaccacgga gtgggtggcg
     2341 atatctggtg gacatatcgc acgtttggcg acatcacagt ttggtttcca ccgcccgccg
     2401 tgtggaaagg acctacggcc gtggccgccg ttgccacgcc ggtcagtgat ctcggccttg
     2461 ctcgcgattc ggcgggtcgc aaccacctga cttggacaga gcaggggggc aggcaaatat
     2521 tccacgctta tcatgatgat gccggttggt cgaccgccag atctatcatc gcgctcgaca
     2581 caggtacgat tcaatctctg gatatgaccg gcaatgggac gcggctattt atcgtgtttc
     2641 gcgagagtga tggcttgcac tttgctcagg ctggagtcga gcgtcctgct gaatggacaa
     2701 cgcctgttcc tttggcggcc ggtcagactg aggcagttga accggatata ctcgtttctc
     2761 agtcgggcgc gctttttgtg gcctacaccg tcgcacttaa tgagccgcga ggtgcttatc
     2821 tcactcgctc gactgatctg ggcgcgaatt ggtctgaacc aatccagctg ttcagcggcg
     2881 ccgcggccga ttggcctgcg gtaggttatg tcaatttagt tgagacaacc gatggccgct
     2941 tgcatgctgt cgtcgccgag cgggcattgc cgccggacga tacaatcctg cgcctggcat
     3001 atagtcgatc ggaggacggc gggatgacct ggtcggcgac gacaccgatc gtctccacgc
     3061 ctatgcgttg gtcgtcgttg attggctacg gcgagcgcgt tttgcacatg ttatgggctg
     3121 aacctaccaa tgatcgccta ttactgtggc atattcaatc ggttgatgga ggtaattcat
     3181 gggacgaggc tacccaggtt ggtgcgctcg atggaaccga tctgccggcg gtcactatgg
     3241 acccaacggg ccaattacat ctattgggca tggaagatgg ccgactgctg gcctggacat
     3301 tcgacggcgc aaagtggact gcggccgagt cagcaacact gaatttggct gacggtggcc
     3361 tgctggctgc ggaggtcgat agcgccggaa atctcgtcgc tgcgtatggg ctgcttgtcc
     3421 ccggctcgtc gccggaagag gcgacgggtg gcctgttcgt catgactcgt ccgttggatt
     3481 tgccggccgt tgcgctgccc accccacctc ctccgccggc cacaatggcg cctgagccga
     3541 ccgtcgttct gacggccacg cctgagccaa cgccaactgt catcgtgccg acagcacccg
     3601 acagcaacct tctatccagc gtgcccggag ccggcagccg tgctgggcag ctggccattg
     3661 ccgtgatccc ggccgcgctg gtggtgttga ttgcgagcat cattggcctg cgggtcatgc
     3721 gcagaggggg gcgttgatgg ccgggaagcg gcctgcagtc atggcgttgg cacttctttt
     3781 gacagtgctc tttttcccgt tggctggcta ttcgatcgcg caaaatcagg gtgagtggtc
     3841 aaaaccagag ctcatatacg agacagaagc ggcgataaat tatccatatg tggtcggcga
     3901 tggaaccgga gtcacgcatc ttttctggcg tgaatttgat gaagatgagt cggatagcga
     3961 ctcgggcttg caggcgatta tctattcaaa tgatattggc ggccgttgga acccagggcg
     4021 cgatattatc gtcatgaaaa gcgttgtcgc tccagccgcg gcggttgatg ccaatggttt
     4081 aatccatctc ttatggcaaa gtaccaataa tacgctctat catagcctgg caacgacgga
     4141 gtcagcccac tcggcccaag gctgggctca accggaaccc attgccgcaa ctaattcgag
     4201 cgctcatata cagacaggca tcgacaatgt gatgcatgta gtgtttccag ggatagacac
     4261 gaccggcgtt tattatattc gttatgataa cgagacgcat ggctggtctt tccctgttaa
     4321 catttccgct cccgcctcct caaacacctc aagcgacttt acccgtttgg cgattggatc
     4381 ggacggcacg ctccacgtcg tttggacgga gtatttgctt cccggcggct ggccaccgac
     4441 tggcatctac tacgcccaat cgactgatgg tggcgggacg tggtcccggg cgacggaact
     4501 ggaaggtgca ggcagcgtgc aagccaacgt tgctgtcgac agccgtggtc gcgtccatgt
     4561 agcctggaat cgcatggccg gcatcggcgg ccgataccac cgatggtctg aagacggtgg
     4621 ccaatcctgg tcaccattgg ccgagattgc gcctgccggg cagggtggca cagaaggccc
     4681 accccaatta gttgtcgata gtgccgacgc acttcatctg ctgaccacct tcaatgggtg
     4741 tgcccaatat gcaacatggg tagagggtgt gtggacgtcc ccggtatgca tctccggacg
     4801 agaggcgatg gccagtggct ttatcgagtc tccgactatg actgtgacta atggtaacca
     4861 gcttcatgcc gtattttggg acgatcggaa acggctttgg cacgcgacga tgacgaccaa
     4921 tgcgccgttt atcccatcgg tagcgctaat agaatccgcg ccgccaacaa tcgctgccag
     4981 ccctgctgcc gttgaggtaa cgcagtccgt ggcgacacca ataatcaatt tgtcgacagc
     5041 gccgccaggc gacggtgaat tgctcggccc ggctcaagcc ttgttaccgg ccgtggccat
     5101 cgccggcgcg ttgctgggtt tcgttatact gatcaagtta ttgcgatctc gctaactttg
     5161 ctgatcacat ggcagaacaa tccaagcgct ccccccttta taccagtgcc accatcgatg
     5221 tggctgatgt tgtctcgcga ccggcattga tcatcgaacc ggcgcgcggc cacttctctc
     5281 tgcaactgga tcgcctgtgg gcctatcgcg agttgctcta tttcctcgtc tggcgcgatg
     5341 tgaaggtgcg ctataagcag acggcgttgg gcgtggcctg ggttgtcctg caaccgttgc
     5401 tgacgacgct gattttcacc gtcctgtttg gattgctgct caaggttcca tcgggcgaca
     5461 tgccctaccc agttttcgca ctggcgggat tgatcccatg gcagtatttc gccggggcgc
     5521 tgacgcgaac ttcgtccagc ctggtgggca atgcccatct catcaccaag gtctactttc
     5581 cgcgcctaat catcccgctg accggcgtgt tgagcggcct ggtcgattcc gctatcggtt
     5641 tcgtcctgct ggcggcgctg ctggtcgtct atggcatttg gccgaccgcg gccattctat
     5701 tgttgcctgc cttcttgctg ctggcgatgg ccacggcgct gggcttcggg ctgtggctgg
     5761 cggcgctgaa tgtgcgctat cgcgacatcg gctatctggt gccgttcctc atccagattt
     5821 ggctctacgc gacgccggtg gcctatggca gcacgctcat cccggagcgc tatcgctggt
     5881 tgctggggct gaacccgatg acgggcgtgg tggaagggtt tcgctgggca ctgttggggc
     5941 aagagccgcc ggggcgattg ttcgcgctgt cggtgacgat tagcgtcgta gtgctgctga
     6001 ccggccttgt ctatttccgc cgcacggagc ggacgtttgc ggatgtggtg tagcgccgga
     6061 ttcctatccg gcaacggcaa gaattaccta tgagtgacat cgccattcgt gtggagaacc
     6121 tgagcaagcg ctacgagatc ggccgggctg ggacacgcca cgatacgctg cgcgacgcgc
     6181 tggctgggct gttccggcgc gaaggccgcg gccgcagcga gacgttctgg gccttgcgcg
     6241 acgtgtcgtt cgaggtgaag cgcggcgagg tcgtgggcat catcggccgc aacggggcgg
     6301 gcaagagcac gctgctcaaa gtcctgtcac agatcaccga accgaccggc ggtcgggccg
     6361 agatccacgg tcgcgtcggt tccctgctgg aggtcggcac cggcttccac cccgagctga
     6421 ccggccgcga gaacatctac ctcaacgggg ccatcctggg catgcgccgc gaggagatcg
     6481 cccgcaagtt cgacgagatc gtcgccttca gcgagatcga gaagttcctc gacacgccgg
     6541 tgaagcgcta cagcagcggc atgtatgtgc ggctggcctt cgccgtggcc gcccatctgg
     6601 agccggagat tttgctggtg gatgaggtgc tggcggtggg agacgcggcg tttcagaaga
     6661 aggcgctggg gaagatgggg gatgtggtga atgaggggcg gacggtgttg tttgtgagtc
     6721 ataatatgga tgctgttcaa cgactctgca atagggccct gcttgtcgca aatggccgta
     6781 tcattactca gggggatgtg aagacgtcaa ttcgggaata tcaacgagag caacttaccg
     6841 tcaggaatgc ttttgcctgg cacgatattt ctcaagcaca gcgcacaggt agtggggacg
     6901 taaaattcag tgctgtgaag ttgtgcggtg ggagcctgcc cgtggacgca atttttaccg
     6961 gtgggccact gtccgtagag ttggatattg tgtcaaaaat ttccacaagc gtatcgagta
     7021 tggccatcac tgtctttacc ccaaatggga ccatgctcgt gaatgcagat acgatttcgc
     7081 aaggcaagac tttcaatctt cgtgaaggca ggacccgagt ccaatttgtc gtttatgcac
     7141 ttcatttgtc acccggcacc tataatcttg ggttgtggct ggctaaccat cctggaatta
     7201 ttcatgatag agtgccgaca gctattcaat ttgaggttgt cgaatatgga cagaacaatc
     7261 aacctggcgt aagggtggat ggtctggtgc cttgttcgtt caaggtggaa gcacttgatt
     7321 ttgttcccga tggtgacgaa tgactctgtt cgtccccatc gattggctgg agattcgccc
     7381 agcaaaaccg tgtatctaac ccatatgcaa atcttgaaat caggcagtcg taataggctc
     7441 gtcttctttt tggccattat tacgctatca tttgccctcc ttttcttcat aacacgtgca
     7501 ttggatgagc ggcttgcgct gatggcgagc gtcgcctggg ccacactact catcatctat
     7561 ctgatgtatc gttggcgatc gattccgccg atttacgatc tgcaactaat cgaattacgt
     7621 catcttattg aattacagtc gctgatgggt ggtgcatttt tgccgttctc agtctgggcc
     7681 atggcaccca acgatctatt ccaactggtg tcgaccattc aaatacaacg atatgggacc
     7741 atagttgaat gtggttccgg tgtgtcgact atcacgatcg gccgcttgtt gcgtcaatta
     7801 gggatcggcc atttatattc gctggaagag gacaaggcat ggtatgaaat gatggtcgcg
     7861 acgttggctg ccgaagggct tacaggcgtc gtcacgctca ttcatgctcc acttgagttg
     7921 tatcaaggcg cagacgccga atggtatgcc ctggagcaag tgcgacaggt tcgggctggc
     7981 gcaggccata ttgatctgct gcttgtagac gggccaaaat ctgtgtccac ctattcgcgc
     8041 tacccggcac tgccggtttt tgtcgcccaa ctggacgggt ctagtctcat cgtgctggac
     8101 gacacccgac gaccgggaga aaccgctgtg ttagagcgtt ggagccgcga gtttcagctg
     8161 cgatttgagg atcagccagg cagcccgcgc ggccaggcct acatacgttt agttaaagaa
     8221 ctatgaatca tcctcttgtc agcatcatca tccccaccta taaccgcaag gagtatattc
     8281 agcaggcgat cgatagtgta atagtgcaga cctacccgca ctacgagatc atcgtgattg
     8341 atgacggctc caccgatggt acgggcgagg cgttggtggc gcgctacaat gatcggatca
     8401 tatatcatcg gcaggaaaat caaggggagt cggtggcgcg taattggggg atcaatgtgg
     8461 cgcgaggaga gtacgtagga tttcttgatg atgacgatat atatctgccc cggatgctgg
     8521 aagtgacgat cgagatttta gaacaacatc ccgatatagg ttttgttagc gttcaatatt
     8581 atgtcactaa cgaggcggga gatattctgt acaccccgct tctttccaat agttgtgatg
     8641 gatatatcac agctgaagag ttcctgcttg gagattcaac cgtacctagt aactcaatat
     8701 tacgtcgaaa agtgattgat caggctggat atttcgacac gacgattcga tatggggagg
     8761 atgcggaatt tttccgcaag atatttgcac aatttcgagc gtattttctc gcagagccat
     8821 tgatggcgat gagggttggg gaagcgaggc agtcgggtcg catactcgat cctgccaagg
     8881 cgaaacttcg atttgatgat cagaccgcag cattgtcaag aattgaagat gatcccgtgt
     8941 ttgtcccata catcccgaaa ttgtgcggtt taattaacgc caattatgca tttaacctga
     9001 ttgccaatgg agagacgtca aaggccgagc cgatattaaa gcatgtgttt acgctggaac
     9061 cgtcagactg gttctataag gaaattatgt gcgggctttt cctgcaatat atcgaggcac
     9121 ttgatgatga acagggcatt gctgcagtga tgaaattcgc acagcaggtt tttactgctg
     9181 ttgatggaac ccgggcgttc aggcaagagt ggaggcgtaa aattataggc aaattccact
     9241 tgaatcggtt tttcaaacag gcatttcaag aagaatgggc cgagatgcgc ctttcctatt
     9301 tccgggcaat tgccaatgat ctcgtctgcc tgaaaaaccg cgggatacat tctttcatga
     9361 tcaagtcatt cattgggcca cgtctgttac gaactcgcgt cttttagagc caatcaggtt
     9421 tctccacttt ttcaactgga atgccaacaa ttagcgtcat catccccacc tataacggtg
     9481 cgcgctatat ccggcaggcg attgatagcg tcctggcgca gtcatatagc gattatgaaa
     9541 ttatcgtcgt tgatgatggt tcgaccgata acacggccga gatcttgtgg ccgtatggtg
     9601 accgtatcac ctacctctac caatcaaatc aaaaactgcc cacagctcgc aataacggga
     9661 tcaccgcgtc gaaaggcaaa tacctggcct ttctcgatag tgacgatctc tttctgcccg
     9721 acaagctcga ggttcaggca cgctatctcg atgaacggcc ggacgtgggg ctggtggcgt
     9781 ccggctggca atatatcgac gacgatgggt ggatcttgtc gacgagtgag cctcaggaag
     9841 atcggccgat cactctggag acgatattat ttggcgggct ggcccctgtc catgctgttt
     9901 tgttgcgtcg tgaatggttt ggccgcgttg gcggcttcga cccccaattc gcctattgcg
     9961 aggatatgga cctgtggtat cggctggcgc tggccggatg ccccatggtt tgggttgagg
    10021 ccatcgtttg ccaataccga atccataccg ataacatgag ccgttcacct gaaatccact
    10081 ttaattatta tcgccgcgcg atggataaag cttttgccga tccacgattg ccaaaagagc
    10141 tgcttgcccg gcaggccgaa ctcgatgcca acctggactt gtccgaggcg tcacgactgg
    10201 tcgccggcgg atggactgag gacgctcgcg aacgcgtgct tcgcgcagta agtattaacc
    10261 ccagacttaa ggatgacaat ggccatgggt tggcggagat agtcgtagga ttgcggtcga
    10321 gtgtatggag cgacggtcgc tttgccgagt tcgtggcgac ggtcatggct gatgagatcc
    10381 ctgccttgga ccacacaatg gcgattgtct ccgcgaagaa gtgcttctat aacgcataca
    10441 acaaacagcg agtgaccgac attcggcgag cgtggttcga catcatctgc cgcgatccgc
    10501 gctggttggt cgaccgcggc ggttggtcga ttctgggtca atcgttcgtc ggcttcaaac
    10561 aggcatccgg gacgagggaa taggggagcc ggaagatgtc agatctggtt accgtcatac
    10621 ttacgaccct caacagcgaa aaatatctcc gcaaatccat tgacagttgc ctcaatcaga
    10681 ctcatcacca tatcgagttg ctcgttgtgg atggcggttc gacggatgcg actatagaga
    10741 tcgttgagag ttacatcaag gcagaccccc gcatccggct catctaccag gaaaacaatg
    10801 cgggcaagct ccccggagcg atcaacctgg gcatggccaa tgcccgtggt gaatatatca
    10861 cctggacgca ggatgattgc tggtatgagc ctgatgcatt ggagttgatg ttagccttct
    10921 tacgagatca ctccagggtt gcgctggtct atacagatta ttggcttgtg gacaacctgt
    10981 atgggcaaca ggcgatcaag tacacaagag ttagcgatgt cgatcggatg cttgatgaag
    11041 atgtcatcca gcagtgtttt ctcttccggc gcgaggtgta tgaggcggtg gggcctcagg
    11101 atatacagta ctttccaatc cacgaagttc cctggcgact caagatcacg caaggattcc
    11161 ggatcgagcc tctccatatc ccgttgcaat actatcactg gcatgtcgat tcccttaccg
    11221 gacggatagg cggttatgag ttgcaacgta tgatgtatcg ggcattctta atcgagggac
    11281 acattgatga aggtgaactt cgccgtcgtt tggctgatat tgacatccac gatgcatacg
    11341 ccgcttttgt tttccgtggc gactaccgcg cgtttcgtag cctttttaag gcggccattc
    11401 tgggaaatcc gacacacggc cgcaaccgtg gtttgtggaa attgtttgtg acttcctttt
    11461 tgccggcgcg cgaatggaga cggcaggaga tgctcgacgc ctggcaggct caagaagcca
    11521 aacgccaggt gattcttgtt cacgagttca gggaacgatt cccgcaacgg caagtggctc
    11581 atgccaacga ttagcgtcat catcccttgt tataatcaag gccgctttct gaagttcgcc
    11641 atcgatagcg tcatcgccca gacatttgag gagtgggaag ttattatcgt taacgacggc
    11701 tcgactgacg acacggctca ggttgtggcc ggctacgacg atgctcgtgt ccgttatgtt
    11761 tatcaggaaa accggggcct gtcggctgca cggaatacgg gattgcgtga gagccatagc
    11821 gaatacgtcg ccttccttga cgcagacgat gcctggacgc cttcgtttct gcaacattca
    11881 ctgctaccga tgctaaatga tcagcgtcct ggaatggttt attcccgcta tcgatttctg
    11941 gacgaccggg gcaatctcca gtctcagatt ggcggagagg cgcttgagtc gactcggttc
    12001 cggcgagtga ttcgccgcgg cggctttttc ccgcccaact gtgctttggt gcgaagaagc
    12061 gcaatcgaca aagttggctg ttttaagagc cttccgacag gcgcagagga ttgggatctt
    12121 tggttgagaa ttgccgaaga gtttcctgtg gttggccttg aggatccgct cgcctattac
    12181 cgtatttatc ccgggagcat gtctaccaat gggttgcgaa tgcaaatcag tcgcttcgcc
    12241 gttttggccg aacattttgg cccgcccgat ggccctcccg gcttatggcc ggaggctaaa
    12301 cgcgaagcct atgcctgcgg ttatcgaagt tgcgcctgtg aatttctcca acaaaatgat
    12361 ccggatacgg cgtgggagct attatcggaa ggtgctcgca tctggccgcc ccttttgtcg
    12421 gaggtagaga cgtattacga gttcattctt ggcgatcaac agaaaggcca gagagggcga
    12481 gccgacctgc tggacttacc cgctcgcgaa aaggatgtca tatccaggtt ggatgacctg
    12541 tttgtcggag aggcggatga caaagtgaaa tcgcagcgtc gtgttgtccg cgctcgcgct
    12601 tacctggccc tggcgatgct tgccgatcag gcgggtgatt gggcggcggc acgtggatat
    12661 ttgcgaaagg ctgtgcggct agatccattg ttatgtgttc gaccagaaat aatgagacga
    12721 tttgccaagt tacatgccgg acaacgattt gttcggagat ggaaggcggg taagccggca
    12781 gataggactg ttccacgagg cggataatca ggtcgtccgt taaccaagcg gatcgccatt
    12841 taccacaatc gtctattcat cggcagcaag ttcgcaccac gggaggcctt ggccccacgg
    12901 tacgaactta atgttgacca agtcatattt gcctcccatc gcagtaaaag tagttcgaga
    12961 ttgccgaaaa aatgttcagt caacggctgc gtgcaaaatg aaggttcttt atgttccccg
    13021 ctccgacgcc gatgtcgtca caggcgggga tgtgctctac gccaggtcac tttgtcaggc
    13081 cctctgcccg cttggggtcg aactagtgtg tgtggaggtt gatcatctgg agcacgttgt
    13141 agatgccgat gctgtattcc tcactcaaat ctaccagatt gacgttgccg agaaggttgc
    13201 tcattgggct tcgcgccgcg gaatccccct cctgattagc ccattatatg aagaaggcga
    13261 ccacttgggc tttagactca ctgttcaggg ggaaggcaaa tggtcacgta tatccagaat
    13321 catcggttcc caagctgcgg aagagctata tgtcaggcgt gaggcggctc gccgtacgcg
    13381 acaggacatc tggcaacggc aacgacaatt gctccaacag gcgtatgtgg tgtccaatac
    13441 gcactatgag ctggagcatc tgcgtcgctg gtttaaactg acgaacctcc gtgcaacgat
    13501 tattccactt ggcatcgatc ccggtaaatt caatcttgaa tcagggggcg cgaccgaatt
    13561 tctgcctgct caaatccagg cctggcgcgg aaaatatcta ttgcaggtgg gcctaatcag
    13621 cgcgcgaaaa aatcaactca gccttctgcg ggctatggac gatagcctaa ttcctgttgt
    13681 attaattggt cggcattccc cctacgaacc gggctatgtt tctgaagtaa cccaattagc
    13741 tcgtgctcgt gggactgtgg cgatgcttga acatgtagat ctgagtacat tggcagcgct
    13801 ctatgggcag gcggccgcgc atgtattgcc tagctggtca gagcggccgg ggcttgtcag
    13861 cctggaggca gcggcatgcg gatgcaacgt ggtgaccagc actgccgcac cgatatggga
    13921 atatcttggt caagaagtag atatctgtgc gccaaatagc tcgaaatcta tccgacaagc
    13981 ggttttgaac gcgatgaacc gtaaatcgac cctctcgcta agtaataaaa taacgtctac
    14041 ctatacatgg gaccgcactg ccggcgcgtt tagacaaatg ctgagagata ttatcgctta
    14101 aggacataat tcggcttgcg tatcctcttc ctctccaacg tctatcctcc atatagccag
    14161 ggtggctacg aacagctctg ccaggaagtc gcgcaactgc tggctgccag aggtcacaca
    14221 ctttgcgtgt tggccggcag gacgacagga gcggccgatg tcgaggaaga cggcccggtt
    14281 cgtgttcatc gacgcctgga actggagctt gaaggtggat tgagccacac tatcgcgcga
    14341 ttggccatcg gcggccgaga agcgagcgaa cgtcgaaatg tcgccacggt cgaacgcctt
    14401 atcgacgaat tccagcccga cgccgtcctc atctggggaa tgtggaatgt gccccggtct
    14461 gtgcccgcgg ccgtagagcg gcttttgcct gaacgagttg cctactacct gtgtgattac
    14521 tggcccgtgt tgcccaatgc gtatattcag cgccttgagg cgcctgcccg gcgaggtcat
    14581 gccgattggg ccaagcgggg catcacccag ttgctcctgc ccgcggcagc tgcagaaatg
    14641 ccgacttccc tctcatttaa gcgagctgcg tgcgttagtt acgcgcttcg cgacgctctg
    14701 gtgcaccagg gaacgcctct cgataacgct caaattattc acaatggaat ttatttatct
    14761 gatttctcgg tcggttcaga cgccccttca acgaataatt cgttacgatt gatttattcc
    14821 gggcggctat ctccggaaaa gggtcttcat gtcgctctgg aggcgctggc tttgctggat
    14881 ggatcagtgg ccaacagatg tgaattactt atcgccggtg gaggtgatga ggattatctc
    14941 cggcacttgc gaaccattgc cacaagagga tcggtaccgg tcacattcct gggacatgtg
    15001 ggccgtgcaa atatccccag attactctcg gaaagccagg ttctgatctt tccctcggaa
    15061 tggggagagc cattggctcg ggcggtcatc gaagcaatgg ccgccggcct cgctgtcatc
    15121 ggcacgacca ccggcgggac aggtgaaatc ctggtagaag gtaaaacagg cctcactttt
    15181 cgccccggcg acccagccga cctggcccgg cagatcacct ggttggcggc cgatccgcga
    15241 ctgcgtgaac aattggcggc cgccgggcag gcgctagttc ttcgtgagtt tgatatccga
    15301 tccacagtcg atcaactgga atcgttgctt tgtcaggcga ccatccagtc tgaatctcca
    15361 gtcgcatgaa cctcctctac ctctcctcct ggttcccctt cccaccaact aacggctccg
    15421 aactgcggat caacgcgcta atccgggggc tggcggcgcg gcatgatgtg acgctgctca
    15481 gtttccagcg gcggccgatt gatccgcgcg gtctggccca agcacgcgcc atactgtcgg
    15541 atgtcagact ggttccatgg cgcgagtttg ctcccgacag ccgccgcgct cgcctcgggt
    15601 tcttaagctc ccagccgcgc tcggtagttg acacctattc gaccgaaatg gacatgctca
    15661 tccgcgaggt gattgccatg caccaatacg atgccgttgt cgtctcgcaa ctggcaatgg
    15721 ccgcctattg gccggcgtgg ggcgatctgc cgaccgttct ggatgaggtc gaactggggt
    15781 cttctcgcga acagtctcgc ttggcctcga cccctatggc acgcgtcaga cgtgccctca
    15841 tgtgggcaaa gctgcaatcc tatgtgcgtg ggctactgcc ccatttcgat gccgtcaccg
    15901 tcgtctccca acgggagcgc gatctgctac gacaggttgc gccggactat tcggccgtct
    15961 cgattgtccc gaacggaatc gatttggccg cctgcgcgag tgttcgtgca ggccatgatc
    16021 ctgacacgct gatcttcacc ggtgctttca cttatgatgt taaccatgag gcgatggtct
    16081 ggttcctgga cgaggtctat cccatcatcc ggcagaatgt accgcagctt cgattaacca
    16141 tcaccggcga ccatgcggga aagccgcttc cctcggctga cggcgtcacg ctgaccgggt
    16201 tcgtcgatga tgtgcggccg ttgattgccg gggctggcgc ggccgtggtt cctatcaggc
    16261 agggtggtgg aacgcggctc aagatattgg aggcgatggc tgttggcacg ccggtggtag
    16321 ccacgagcaa gggagcagaa gggttggacg tgactgatgg cgagaacatt ttgctggctg
    16381 atacgccaca ggactttgct gaagcgatct tgcggttgcg acaagatgca gcattatgcc
    16441 aaaacctcgt gaataacgct tgtctcctta ttcgacggca gtacgattgg gtagttattc
    16501 tgccggattt catcgagttg gtggagcgcg tcgcctcgcg agcgatgact ccagtccaac
    16561 aatagccagg aatgatgcga cagattttta ctccgcaggc aattcgatca tcgcaaggcg
    16621 cgcgcaatgt gtggcggctg gccctcattg tcgccgttct ccttggcagc gctatcctgg
    16681 cctatatcgt acccgtacag cgtaaggttc tactcgtccc tgctgcgatg ctggccattg
    16741 ccgccgccta tatcttcctg cgccgccctt cccttggtat gatggccctg gtgttcgccg
    16801 gcttgctcgt gccgttcagc atcggcaccg gcaccggcac cagcctgaac ccggtcgtcc
    16861 tgcttgtgcc tgccctgact gtattatggc tggccgaaaa tggcttgcgc catcatgcta
    16921 ttcgactccg tcgccacagc agcgtctatc tcttgctcgt cttcatggct gtggctacgc
    16981 tttcctacat tgtgggccta ctcccctggt tcgatatcca cggcgcaggg gcagcggcgc
    17041 aaatcggcgg gctgatggtg ttctatattt cggctgccgc ctttctcgtg tccgcccaca
    17101 ccctcaacga gcgttggctg gctcggctgg tctatctatt tatcgccatt ggcgcgctga
    17161 tcgtcctcgg ccggtttgta ttacccctcg gccgggtatt ggcgcaattt gtcgatgggg
    17221 ccgcaatggg tagcgttttc tggatctggt tggtggcatt gtcggcggga ctggcgttgt
    17281 tccacgacga tctgccgctg cggtggcgac tggtgctgat cgccgtggct tccctggcct
    17341 tgttcctcgg tctctttcag aacagagaat gggcctcggg ttgggcgcca cccttggtgg
    17401 ccttgctcat cctcgtctgg ctgcgctttc cgcgctgggg ctggattggc ctcttcgtcg
    17461 ccaccgtcgt ttttatcctg gagatcgacc gcttctggat ggcggtcacg accgaccaga
    17521 gctggctggc gcggcagcag gcgtggcgta tcgttctcga cacgacgtcg gtgaatccag
    17581 tgctggggct ggggccgtcg aattactatt tctacgtgca aaatgccacc attcggggtt
    17641 ggggcggagc atggaacgtc aagttctcct cgcacaacaa ctgggttgac ctgattgccc
    17701 agacggggat tcttggcacg gccgtctttg tgctgttcac cttgagcttg tttcgcgtcg
    17761 ggatgcgcct ctacaccggg caggccaatg gcttcccccg tgcctacgcg gccgcgtgcg
    17821 tcgccggtct tgtggccacg ctcatcagcg gctttctggg cgactggttc ctgccgttcg
    17881 tctacaatat cggcttggcg ggactgcgag ccagtatcct gttttgggtg tttatggggg
    17941 ggctgttgat gctttatatg gaacaggaga acagttctaa tccttgaaaa tggaactgtc
    18001 ggtaatcatc gtcaactgga acacccgtga tcttctggct caatgcctgg agtcggttta
    18061 tgccaatcca ccgaatggtg agttcgagat cattgtggtt gataatggtt cgtcggacgg
    18121 cagtgtcgac atggtgcgcg agcgatttcc ggaagtccta ttgattgaaa atttggagaa
    18181 caccggcttc gcccgagcca ataatcaggc tattcgcata agtgccggcc gctatatcca
    18241 attattaaac agcgatgcga tggttacgcc ggattcatta gaggcgatgg cgacttttat
    18301 ggattttcat ccgcaagccg gcgtggttgg accaatgctt ataaaccgcg acgggagttt
    18361 tcaggcatca tttgccgagt tcccaacgtt atggactgaa ctttgtcttt taacaggaat
    18421 ttcgcgtttg gtcattggcc cctatgctcc tagcccccgc cccaagctat acgcagttcc
    18481 agaacaagtt gattgggtgg ctggtgcagc catcatggtg cgccgggctg caattgaccg
    18541 ggtgggtctg atggatgaaa cctacgcctt ttatagtgaa gagactgatt ggtgctggag
    18601 gatgaaccag tggggatggc aggtttggta catacctttg attaaagtat accatatcgg
    18661 agctgcgagt tcgcggaagc gttcgcacga aagttatatt cagctttacg atagcaaggt
    18721 gcggttcttt aagacagctt acggcccaag ggctgccaat cgtttgcgat ttgtcataaa
    18781 agtgatggca ttcctccgct tgacggtacg tgcactgatg ctgccaataa ccacggcatt
    18841 ttcaaaatcc cgaacaatgc caagaatcca acaagatatc gccttaatcc gtcacatgat
    18901 ctcgagtgat tgaatcggcc ttcagatgga gaaaaatatc ccttgtgatc tctgtggagg
    18961 agttgtaaca gccccttatc ttgggcttgt acgccattct ccatttgccc ttgtcaagtg
    19021 tttgtcctgt ggattggtat tcctggatcc cagacccgat ccttcggaga ttaaatcata
    19081 ttattcccct ttctattaca cgagctgtcc cccagaaccg gctgataatg gaatgaggac
    19141 ccggctaaag aagttagcct atgccggccg atgtagcgac ccgacacagt ggcatcgaac
    19201 agcaaaaata gtaatgccca tcgctcgttc acttattgga tggcgcgcct accggcgggt
    19261 acctccacgc accaagggaa acctgctcga tgttggatgt ggcactggag agcaggcagc
    19321 ctggctccga gataatttgc ttaattggga ggtggaaggg gttgaaataa acagtcatgc
    19381 agcgcaaatc gccgaaagtt tgtttaatct aaaggtttac cagggccacc tgcctgatct
    19441 aaacctccca ccagaaaagt atgatatggt gtccttctgg cattcattgg aacatactta
    19501 tagtcctaat gctaccctga aagagacatt tcgcttgctt cgtcgaggag gttgggtggg
    19561 gatcgaagtt ccgaacattt gcagttggga ggcgcggcaa acaaaggagc attggtatca
    19621 tctggccatt ccagttcacc tttaccattt ctcggtaatt acattatcag cgttgttgaa
    19681 gcagaacgga tttacaccaa tttcagttga gaaggtgcgt ggccgtgctg ggcttgataa
    19741 ttggatcgaa cataatcgcg ccaattattc ctggacccaa gggatcgccg taaaggcagg
    19801 cgtgcaaacg cttggaagat ttagcccatg ggggatccga atttatgcag cgaaagtttg
    19861 acgccgttag caatcgcaaa ccatcagttg atatgtcaat attgatagtt aactggaatg
    19921 cacaagggct tctctctcgt tgtttggctt ctttagaggg tagcgttgga atcagaatag
    19981 aaacgatcgt tgttgacaat gcgtctatag acaacagtgt tgcgatgatc cgagcgaatt
    20041 ttcccaacgt atatgtgatt gccaatggtg aaaacaaagg ttttgctgct gctaacaatc
    20101 aggcagctgc tcaggcaacc ggccgttatc tcttgttatt aaacccggac acaaaaatac
    20161 cagatggctc attagcagaa ctgatcgcat atagtgatac cattgctcaa cttggtgctg
    20221 ttggtccacg tgtaatcaat cccgacggta ctacacagcg ctcctgctgg cgcggctacc
    20281 cgggcatcgc cgcggcgctc atagatgcat tctatttgtg gaaaatcccg gggctaccct
    20341 ttgtgcgaaa cacggaaata tcggccgatc atttacgaaa tgttctggaa gtcgatcata
    20401 ttcttggtgc gtgcatgcta atcccacgag acgtatggca ggaaatagga ccacttgatg
    20461 aggactactt cctgttcctg gaagagaccg attggtgccg tcgggcaaag gctcttggta
    20521 agcggatagt ctatcttccc actgctgaag tgatccatta cggccaacat agtatgcgcc
    20581 agcagccatc gcgcaatttg ccgcatcttt atcgtagcta ttgtcgcttt tatcgtcacg
    20641 gatcgggtag cgatggtttt cggatgtttg ttttgaaggt cgtgatctcc ttagccattt
    20701 tggttcggtt aatcatgtgg caaattcgcg gatttttctc tcatacagaa aagcaacgcg
    20761 aacaagcgcg tgatatgctt gtcggctatt ttcaagtatt gcgtgaacta ccttctatgt
    20821 gagttgcatg cgtatcctct tcctctccca actcatcccc tacccgcttg atgccgggcc
    20881 gaaggtgc
//
LOCUS       DAOMQG010000021        20654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1116_length_20654_cov_11.005194, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000021 DAOMQG010000000
VERSION     DAOMQG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 20654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20654
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1116_length_20654_cov_11.005194"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PLC06_02550"
     CDS             complement(<1..1301)
                     /locus_tag="PLC06_02550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRO89355.1"
                     /translation="MEFQPPFIDYSVLWPFIFVAGTGLLLMIVDLLANGERSRRWAPV
                     LTIAGLIGSLVTSLLLWNDGRSAFSVSGGQPMLVADNFAVALNVIFALTGILTVLLSV
                     NYLQRTGHDRPEFYMLMLFSLSGMMLMGMANDLILIFLALELLSIPLYTLAGSAWPRP
                     ESEESALKYFIIGAFSSAIFVYGIALIYGATGTTSLPGVLAAAGGGTTTLLLAGAAMM
                     LVGFAFKIAAAPFHMWAPDVYEGAPTVVTAFMSVGAKVGGFAALLRILMVALPNLGET
                     WAMAVSVMAALTLILGNVLAIAQPNIKRMLGYSSIAHAGYILIAVAAAVNSPDGISAA
                     LFYMFAYTFTNLGAFAVAVAVERKEGEGILIEDYKGLAKRYPWMAAAMTVFMLSLTGV
                     PPTAGFAGKFFVFRAGIEAGLTWLVLIGVVTSVISAYYYLR"
     assembly_gap    1345..1444
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1542..1772
                     /locus_tag="PLC06_02555"
     CDS             1542..1772
                     /locus_tag="PLC06_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HRO89356.1"
                     /translation="MSDSWLTTLITDTPQEGFELAITLSRRGVKYTQPDVAVLKQLRP
                     EYANSADSLTAASQVIAINFQTVAAANNYWRK"
     gene            1873..2202
                     /locus_tag="PLC06_02560"
     CDS             1873..2202
                     /locus_tag="PLC06_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRO89357.1"
                     /translation="MTWIYLGIAIVSEVIATSFLKLSDGFTKLWPSVVVVIGYGLAFF
                     FLSLTLRSMSIGVAYAVWSGVGIVLTALIAWVAFGQKLDAAGFVGIGLIIAGVIVLNL
                     FSKSAGH"
     gene            2405..5161
                     /locus_tag="PLC06_02565"
     CDS             2405..5161
                     /locus_tag="PLC06_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRO89358.1"
                     /translation="MIRPPSGTVTFLFSDIEGSTPLWERHPKSMEAALPRHDDILRQA
                     ITIHNGYVFKTVGDAFCAAFSRPDDALAAAIEAQRRLSNEPWPADIGQLRARIGIHSG
                     TAVERESDYFGPAVNRAARIEAAAHGGQILLSQAVERLLGGELPEGIDLIDLGEHRLK
                     GLGRPERLFQVTAVDLQSDFPSLALDRQRTNLPAGEVELFGRDRELTELVSLLAGRRV
                     RLVTLTGPGGVGKTSLALKAAIALLPHFPDGVYQVLLTSLGNPTAVPSAVAAVLGARE
                     MPDKPPAEVVRDVLREKHLLLLMDNFEHILPAAGHLSEWLAVAPGLVVLSTSRSPLRL
                     RGEHEFPIAPLPTPDLITDPTMLGHEAAIALFMERARAVRPGLALTAANAGVVSAICR
                     RLEGLPLAIELAAARARLLPLPDLLRRLESALPVLVGGPRDSPERQRSLRETIAWSYR
                     LLAPGQQRLFRSLAAFRGGFSLEEVAAICETGTPLDALEGLEPLAAQSLIRADETGSD
                     ARFTMLETVREFALEELTAAGEMEYVRSRHLDYFTDLARQLSDAMRGNEYQVWFNRLN
                     AEQDNVRYALQWALDDNRSSAIAERGAWLAGYLGFFWLFGNSIDEARRTFKRAVERVS
                     RPCPARAKALVGAGIFAWQTGEYAAAETWLHEGLAIWRSVSYPNELADATHMTGHLEF
                     DRRRYEPARALFAESLDLFKSLGNAVQVATLTADLGLVAMQQADYPLAEACYQEALSK
                     YRQNDIPEALSDVLYRLGDLYRLRNDIDGAAAYYQESLDRIRPLNYKLGIACGLHKLG
                     QVARLTGQPQIARDRLREALVLQHEVANKQGIVECLAALGGLALEDSQIGRAVQLLGA
                     AEALLAELDAPLSPPDRDQLLRDVAAVREAVGAGNYHGPVDTGNPLSWESAVELGLQV
                     QSSD"
     gene            complement(5164..6063)
                     /locus_tag="PLC06_02570"
     CDS             complement(5164..6063)
                     /locus_tag="PLC06_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRO89359.1"
                     /translation="MEALSYLAFRISYFVIMEPVYFVHLSDTHFGPTEEYRRQGHRSL
                     PYARHVIDMLNRLPTRPDFVIHTGDVTTHPTQAAYRLAAETFAALEIPIYYCTGNHDR
                     SADIHRYLNMGPKVDCQPGTDTLSYTFEVRGERFLVLDARGPDQIDPHGLLSPQQMDV
                     LRAEATPDGPPLCVFTHYPALRLNAPWMDANMLIFNGEEMHQALLPARGRLRGVFYGH
                     IHNSMQTIRDGILYCAVASTFAGFTTWPTEEMIRADHAAMPAFNFVQCLPEQTIIQQR
                     PFARLPGEPEHRPATKDPENLEG"
     gene            complement(6097..7611)
                     /locus_tag="PLC06_02575"
     CDS             complement(6097..7611)
                     /locus_tag="PLC06_02575"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRO89360.1"
                     /translation="MNWLLTILTFLPLVGVLAILFVKESEDGSNRDTIKWIAIAASAV
                     TFAMSLFVLFRFDATNPALQLIDRVDWIPSWGISYFMGVDGLSILMVLLTTFISLLAT
                     IGSWSAIQTQLKAYYIFMLLLEMGMLGVFLAQDLFLFYVFWEFTLVPMYFLIGIWGGA
                     RRVYASIKFFLYTMAGSLLMLVAILYMGITNQTFALPDLIAGREAFAHAQNLLFIAFF
                     VAFAIKVPVFPFHSWLPDAHTEAPTAGSIILAGVLLKMGTYGFMRFNLPLFPEASSHY
                     APIIAVLAIIGIIYGAIVSFAQTDAKKLVAYSSISHLGFVTLGIFSLNSAGIQGGILQ
                     GVNHGLSTGALFFVVGMLYERTHKREMSVYGGVWSVMPLFSALSLIIVLSSMGLPGLN
                     GFVGEFTILLGSMGAESLGPHPWIYTAFATTGVILAAVYLLWFFQRLFMGPMTTDEVR
                     TLPPMNRRELAVLLAFLVFIFWIGIAPAGYFHLMDSTVNTLVNDIGSALLAVAP"
     gene            complement(7626..9869)
                     /locus_tag="PLC06_02580"
     CDS             complement(7626..9869)
                     /locus_tag="PLC06_02580"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRO89361.1"
                     /translation="MNTFSLAPLILYLPLAGLLFNGLFGRGIVDRNRKSGERFVGWLA
                     TLMTFGAFVVSLTLLVSLATNGFEAWYVPIMDWINIPGANFQVGWTQQVDTLSVTMML
                     VVSGIGTLIHIYAIGYMHDDPDFSRFFTYLNLFVFFMLILVSASSYLMMFVGWEGVGL
                     CSYLLIGFWFGRVDKNGAPANSNAARKAMVMNRIGDFGVLLAMFLLFWTFGTLDYTPI
                     FDGAVEMYKANHVVQFGGMSLSLPTVLTAVTALLLLGAAGKSAQIPLYTWLPDAMAGP
                     TPVSALMHAATMVTAGVYLLVRSNVLYEVVRDSGALILGLISTPDLVAWTGALTAFFA
                     GLIAFSQNDIKKVLAYSTVSQLGFMVAAAGMGAYVAAMFHLITHAFFKALLFLGSGSV
                     IHGMEHGHHEIAHGHGDDGDGHDGHDAHHEDDGFDPQDMRYMGGLRNRMRVTYITYMI
                     GALALAGIFPLSGFWSKDEILLHAQTHEMPIFILLALAALGTAFYVGRQLIMVFFGKP
                     RHAAAEHAAESPALMTRPLIVLAVLTALGGLLNAPYLTKAMAERNEMHPRGFWFLLEQ
                     WGEHSIPAIHLTEEGVVHMPHTPVVLSPVVAGLSMLLAVGGLLLAWLVYRGRPRTWDE
                     ADRLARTPIWWWNILPLNTFYSRGLVPLFNRFAAWSAFKLDGEFWHDFIHDRVIRDMF
                     VTFAAFSSNVLDAQGVDGVVNGAGAVTRRLAGVLRLTQTGYARTYALAVFLGAVGLLV
                     YFLFMAN"
     gene            complement(9949..10260)
                     /gene="nuoK"
                     /locus_tag="PLC06_02585"
     CDS             complement(9949..10260)
                     /gene="nuoK"
                     /locus_tag="PLC06_02585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRO89362.1"
                     /translation="MPSAVDINWYIALSGILFVIGALGVLLRRNAIILFMSIELMLNS
                     ANLLFVAFARHLGDLDGQVLVFFVITVAAAEVAVGLALIVAIFRTRKSINIDEINLLK
                     G"
     gene            complement(10253..10780)
                     /locus_tag="PLC06_02590"
     CDS             complement(10253..10780)
                     /locus_tag="PLC06_02590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRO89363.1"
                     /translation="MIHPILFILLAAVAVAAALGMLLSRNAVHSALYLVLNFMTVAVL
                     YLVLNAPFIAMVQITVYAGAIVILFLFVIMLLGAEKLRGAADEVRGSERYHRYAAVLL
                     AVVLIGVFGYLLFQNSLGSTALSPSIDAGPQALGIALFQSYVFPFQVTGVLLLAAVTG
                     VVIFGHTKKRGKDNA"
     gene            complement(10875..11375)
                     /gene="nuoI"
                     /locus_tag="PLC06_02595"
     CDS             complement(10875..11375)
                     /gene="nuoI"
                     /locus_tag="PLC06_02595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRO89364.1"
                     /translation="MIGEILKGMGTTLKHLFIQPVTIQYPEVERPVRPRFKGRHELKR
                     YDNGLEKCIGCALCAAACPADAIFVEAAENTDQERYSPGERYARVYEINMMRCIFCGY
                     CEDACPTEAIVLEHQYELSFYDRASAIYTKEMLLVDAPVNGIPTPQATEPGTFTRAIP
                     DMENPK"
     gene            complement(11450..12553)
                     /gene="nuoH"
                     /locus_tag="PLC06_02600"
     CDS             complement(11450..12553)
                     /gene="nuoH"
                     /locus_tag="PLC06_02600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRO89365.1"
                     /translation="MTPGQIAIRALILGFIISLAVITGFAYTTLMERKVLGRLQARYG
                     PNRTGYIPIPWRGGEKRFLSGFMQPAADAVKLFFKEDPTPAKVDRVIYNLAPMLAVIP
                     AILILAVIPWAPAFRLGPWKFQPYFAIAPGIDVGVLFILAITSIGVYGVVLAGWASNS
                     KYAILGGIRASAQMISYELALGIIILIPIMMADSMDLGVIVDAQRPVWYIFIQPLAAL
                     VFYIAALAELQRAPFDLLEAEQELSAGFNVEYGAMRFGMFFMAEYMKMISLSAIFATF
                     FLGGYRGPFVDQFPWLGIVYILGKIIASLFVMIWIRATLPRFRYDQLMGFGWKTLLPI
                     AVLNFIVTAVLIVMAEEGTLTPLIESVKLFFAG"
     gene            complement(12647..15220)
                     /gene="nuoG"
                     /locus_tag="PLC06_02605"
     CDS             complement(12647..15220)
                     /gene="nuoG"
                     /locus_tag="PLC06_02605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRO89366.1"
                     /translation="MSNMVNLTIDGVEVSVLRGTLVVDAAKKIDIDIPVFCYHPKLAP
                     VGMCRMCLVEIGMPVIDRATGEKVLNADGSVKLNFGKGLQTGCTVAVGEGMVVRTQTP
                     VVKHAREDILEFLLTSHPLDCPVCDKGGECPLQELTMAYGHDEARIDFRDKIKLDKRV
                     PLGELIVLDRERCIQCARCTRFQAEIVDDPVIHFHNRGRRLEIVTFSKPGFDSIWSGN
                     TTDICPVGALTSADFRFGARPWELTPVASICTQCPVGCNLTLNTRREAIAGGRNVIKR
                     IMPRQNEAVNELWICDKGRFVHHFADAPDRLRQPLVRRDGRLEPVSWDDALDYVAGKL
                     QQHKNGAAGITGERLSNEDYYLFQRLFRQGLGNGNIDLVQRNLAGGDVVAQVGLARGS
                     NLKDLKKGDAILVVAADLHEETPVWWLRVKQAADRGATVIVLNARPTRLDKFASHLLH
                     YAPDGTLAAVRALTDGTAEGDLAAAANALANAANLVIFYGAEGLTYDETDTLARMLGN
                     LLLAKQGDGDDATARAGRVNNGLVPVWAHANTQGAWDMGIHPAFGPGYAPTAAAGRDA
                     AEIYAGAADGRVSALYVMGADPVGDGLMDGRGKLAFLVVQELFLTETALLADVVLPAQ
                     SWAEREGTFTSGERRVQRYYPAIPVVGEARPDWQILAQLSERVGLGKPPFAASLAFKE
                     IAKSVEPYRGMDYRTLAHVEAQWPIVGSKDFYYGGMAFENKQGLGQQWAAAAESGAVG
                     NFDLPVEAPHGIPADGFRVLRIPALYTPGTLINHTKLLEKRMARPALCLNPLDAVNFM
                     DGEPLTVQMGGETYDVEAVVDESVPTGLGLLRGVPAPWPAGFEPAIVTRRHAADMVTG
                     D"
     gene            complement(15225..>15524)
                     /locus_tag="PLC06_02610"
     CDS             complement(15225..>15524)
                     /locus_tag="PLC06_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO89367.1"
                     /translation="DDTAIQGPPDLVVEVISPSTAARDRSQKLRAYYQAGVPWYWLVE
                     SDTLLITEYRHTADGYLVNQTAAATDEFAPTLFPGLALRMADLLGPIGATEEVQE"
     assembly_gap    15525..15624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15756..17021)
                     /gene="nuoF"
                     /locus_tag="PLC06_02615"
     CDS             complement(15756..17021)
                     /gene="nuoF"
                     /locus_tag="PLC06_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRO89368.1"
                     /translation="MAHVLLRHREIPDLHKLEVYRQHGGYEGLKKALSMTPAEVIDVV
                     KASNLRGRGGAGFPTGVKWSFIPKNAPDTYVLINTDESETGTFKDRELTEHNPHQIIE
                     GALIAAYAINSHLVFNYMRGEFMDAAYAFEEALLECREAGIIGKNIMGSDYSVEFYTH
                     YGAGAYICGEETALIESIAGNLGQPWSKPPFPAVEGLYRKPTVVNNTETLANVAAILV
                     NGADWFKQMGTADSPGVKIVCLSGHVERPGNYEVPLGTTYRQLIDMAGGVRGGKKFKA
                     LLPSGGSGPIIVADALDAPCSYEGLTPFHSVMGSASLIVMDETTDMVWAALKMTHFFR
                     HESCGKCTPCREGTYWMEKVIDRLYHGGGTEDDIKLLESIATNMTGKCLCALGEFATN
                     PILSSIRHFLPEYKAKVAAGAKKKQAVAA"
     gene            complement(17085..17633)
                     /locus_tag="PLC06_02620"
     CDS             complement(17085..17633)
                     /locus_tag="PLC06_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRO89369.1"
                     /translation="MLRDKYAQEIDALLARFPEKKSAILHLMHLAQNEYGYMSDEAMR
                     EVADILELDPTHVLSLAGFYSLFYEEPTGKYVLEVCNDLACALRGADEFVEMASRKLD
                     IPVDGTTNDGMFTLKTVMCLGACDRAPMLQCNLKFYENLDEAKFGALLTDLRAKAAAG
                     DPEPTVVERIIGYTASDAQNTK"
     gene            complement(17671..18834)
                     /gene="nuoD"
                     /locus_tag="PLC06_02625"
                     /pseudo
     CDS             complement(17671..18834)
                     /gene="nuoD"
                     /locus_tag="PLC06_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
     gene            complement(18979..19500)
                     /locus_tag="PLC06_02630"
     CDS             complement(18979..19500)
                     /locus_tag="PLC06_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRO89370.1"
                     /translation="MTRDDMIVDKLRAALGDAVEQVSDFRGERTVHIRQDKLVDACRL
                     MRDDPELKYNFLSDIVADDYLPDYPRFAVNYHLLSMPNKHRVRLRVWVEDPDDGPPTA
                     ADIWPIATWLEMEVWDLMGVRFAGNNSLRRLFLPEDWQGHPLRKDYPLGYEEVQFSFN
                     WQEIDSKKPYAKQ"
     gene            complement(19586..20068)
                     /locus_tag="PLC06_02635"
     CDS             complement(19586..20068)
                     /locus_tag="PLC06_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRO89371.1"
                     /translation="MGIEQKAGNLGVVTYRLEDVVNWGRTNAMWPMLFGLACCAIEMM
                     STQAAHYDLSRFGMELNRPSPRQSDLMIIAGRVSRKMAPVIRRLYDQMPYPKWVIAMG
                     DCASCGGVFNNYAIVQGVDEIIPVDVYVAGCPPRPEALIDGIMALHDKIRNDKLTDWA
                     "
     gene            complement(20109..20474)
                     /gene="ndhC"
                     /locus_tag="PLC06_02640"
     CDS             complement(20109..20474)
                     /gene="ndhC"
                     /locus_tag="PLC06_02640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRO89372.1"
                     /translation="MEKILIDYLPIAVLFGVAVFFALLLPILSLSLGPKRLGKRALAP
                     YESGMTAIGEANRRLPVKFYRIAVLFILFDVDIILLVPWAVTFRNLGYFGLAMMLIFM
                     TMFIVGDLWVWRKGVLEWE"
BASE COUNT         4089 a         6103 c         6525 g         3737 t
ORIGIN      
        1 atgcgcaggt agtaataggc ggagatgacg ctggtgacga cgccgatgag caccagccag
       61 gtcagaccgg cctcgatgcc ggcccggaag acgaagaact tgcccgcaaa tccggccgtc
      121 ggcggaacgc ccgtcaatga cagcatgaag acggtcatgg cggccgccat ccaggggtag
      181 cgcttggcca atcccttgta atcctcgatc aggatgcctt cgccttcctt gcgctccacg
      241 gccacggcca cggcgaacgc tcccaggttg gtgaaggtgt aggcgaacat gtagaacagg
      301 gcggcgctga tgccgtcggg gctgttgacc gcagcggcca cggcgatgag gatgtagccg
      361 gcgtgggcga tgctgctgta gccgagcatg cgcttgatgt tgggttgggc gatggccagc
      421 acgttgccga ggatgagcgt cagggcggcc atgactgaaa cggccatggc ccacgtctcg
      481 cccagattcg gcagggcgac catgaggatg cgcagcagcg ccgcgaagcc gccgaccttg
      541 gcccctacgg acatgaaggc agtgacgacc gtcggcgcgc cctcatacac gtcgggggcc
      601 cacatgtgaa agggagcggc cgcgatcttg aaggcgaagc cgaccagcat catcgccgcg
      661 ccggccagca gcagcgtcgt cgtgccgcct ccggccgcgg ccagcacacc cggcaatgag
      721 gtcgtgccgg tagccccgta gatcagggct atgccgtaga cgaagatggc cgaggagaac
      781 gcgccgatga tgaaatattt cagggccgat tcttccgact ccggccgcgg ccaggccgac
      841 ccggccaggg tgtagagcgg aatggacagc agttccagag ccaggaagat gaggatcagg
      901 tcgttggcca tgcccatcag catcatgccg ctcagcgaga acagcatgag catgtagaac
      961 tcaggccggt cgtggccggt gcgctgcagg tagttgaccg acagcagcac ggtcaggatg
     1021 ccggtcaggg cgaagatgac gttcagggcc acggcgaaat tgtcggccac cagcatgggc
     1081 tggccgccgg agacgctgaa cgcgctgcgg ccgtcgttcc acagaagcag cgacgtgacc
     1141 aacgagccga tcagcccggc aatcgtcagc accggcgccc agcggcggga acgctctccg
     1201 ttcgcgagca ggtcgacgat catcagcagc aggcccgtgc cggccacaaa tatgaatggc
     1261 cacaggacgg aatagtcgat aaatggcggt tgaaactcca tatgaaacct cataagtagt
     1321 aaaccacaaa tttcataaat ttcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnngctggc taacaatgaa tcaggggcgc tcaacccctc gcgcgaacaa tcccgacaat
     1501 caggcttgca gttcaatcca tatcacacaa ggagtatgat catgtcagac agttggctca
     1561 ccacgcttat taccgacacc ccgcaggaag ggttcgagct tgccatcacg ctcagccgtc
     1621 gcggtgtcaa atatacccag ccggatgtag cggtgctgaa gcagctccgg ccggagtatg
     1681 cgaactcggc cgattcactc accgcggcgt cgcaagtcat cgccatcaat ttccagaccg
     1741 tcgccgccgc gaataattac tggcgcaagt aatgcgccgg gccgcgcctg atatttcgtt
     1801 catatcgtcg ccgccccagg cgcggccggc cttccccaaa acacccgcca accaggaaac
     1861 ttcatcatga ccatgacgtg gatatatctc ggcatcgcca tcgtttctga agtgatagca
     1921 acttcgttcc tgaaattgag tgacggcttc accaaattat ggccgtcggt cgttgtggtg
     1981 atcggctatg ggctggcgtt cttcttcctc tccctgacct tgcgctccat gtcgattggg
     2041 gttgcctatg ccgtctggtc gggcgtgggc attgtgctga cggcgctcat cgcctgggtg
     2101 gcgttcgggc agaagctcga cgcggccggg ttcgtgggca tcgggcttat catcgccgga
     2161 gtgattgttc tcaatttgtt ttcgaaatcg gcggggcatt agggcgacgt cggtcaatcc
     2221 ggcagggccc acgccgggca gagacgggct gcccgacaag cgtcatagtt gttcaacgat
     2281 ctgcgacccc aatcggcagg gcagcatcga ttgcccgatc cgaaaatcca aaaaaccggc
     2341 cgacctctct tcagcttgca gcagcatcat gctacaatga tgctgttgat tccacaggag
     2401 catcatgatc aggccgccca gtggaacagt caccttcctc ttcagcgaca tcgaagggag
     2461 tacgcccttg tgggagcgcc accccaaatc gatggaggca gcgttgccgc gccacgatga
     2521 tatcctgcgt caggccatca ccatccataa cggctacgtc ttcaaaactg tcggcgatgc
     2581 cttctgtgcc gcctttagtc gccccgacga cgcactggca gcagcgattg aagcccagcg
     2641 ccgattgagc aatgaaccct ggccggcgga tatcggccaa ctacgggcgc gcatcgggat
     2701 ccatagcggc acggccgtcg agcgcgagag cgactacttt gggccggctg tcaaccgggc
     2761 ggcgcggatc gaggcggccg ctcacggtgg acaaatcctc ctctcgcaag ctgttgagcg
     2821 gctcctgggg ggtgagctgc cggaaggcat cgacctcatc gatctgggcg agcacagact
     2881 caaagggttg gggcgcccgg aaaggctctt ccaggttacg gccgtcgatc tgcaaagcga
     2941 tttcccatct ctcgcccttg atcgacagcg gacgaacttg cctgcgggtg aggtggagct
     3001 attcggccgc gacagggaac tgacggaact ggtgagcctg ctggcgggtc gtcgcgtccg
     3061 tctggtcaca ttaaccgggc cgggaggtgt gggcaagaca tccctggccc tgaaggccgc
     3121 cattgccctt ttgccccact tccccgatgg cgtctaccag gttctgctaa catcgctggg
     3181 caacccgacg gctgtacctt ccgcggtggc cgcggtgttg ggggcgcgcg aaatgccgga
     3241 caaaccgccg gccgaggtcg tgcgcgacgt cttgcgggag aaacatctcc tcttgctcat
     3301 ggataatttt gaacacattt tgccggcagc cggtcatctg agcgagtggc tggccgtggc
     3361 tcccggtctg gtcgtgttgt caaccagccg ctcacctctc aggctgcggg gagaacatga
     3421 gttcccgatc gcgcccttgc cgacgcccga tctcatcacg gatccgacga tgctggggca
     3481 tgaggccgct attgctctct tcatggagcg cgcccgcgcc gtccgtccgg gcctcgcgct
     3541 gacggccgcc aacgccgggg ttgtctcggc tatctgccgg cgactggaag ggctgcccct
     3601 ggcgattgaa ttggctgcgg cccgcgcccg gttgctgccg ctgccggatc tgctgcggcg
     3661 gctggaaagc gccttgcccg tcctcgttgg cggcccacgc gattccccgg agcgacaacg
     3721 cagcctgcgc gaaaccatcg cctggagtta ccgcttgctg gctcccgggc aacaacgact
     3781 ctttcgatcc ctggctgcct tccggggtgg gttctcgctg gaggaagtgg ccgccatttg
     3841 cgagacaggc acgccgctcg acgcgctcga agggttggag ccgttagccg ctcaaagctt
     3901 gattcgggcc gatgaaaccg gcagcgatgc tcgtttcacc atgctggaaa cggtgcgcga
     3961 gtttgcgctg gaggagttga cggccgcggg cgaaatggaa tacgtcagaa gccgccacct
     4021 ggactatttt accgatctgg ctcgccaatt gagcgacgct atgcgcggca atgaatacca
     4081 ggtctggttt aaccggctga acgctgagca agacaatgtc cgttatgcgc tgcaatgggc
     4141 gttggacgac aatcgctcgt ctgccatcgc cgagcgaggg gcctggctgg ccggctatct
     4201 ggggttcttc tggctgttcg gaaattcgat cgatgaagcc cggcgaactt tcaagcgagc
     4261 tgttgagcgc gtcagccggc cctgtccggc ccgagccaag gctctggtgg gcgcgggtat
     4321 cttcgcctgg caaacgggcg agtacgccgc ggccgaaacc tggctccatg aaggcctggc
     4381 catctggcga tccgtctctt atcccaatga attggccgac gcgactcata tgaccggcca
     4441 tctggaattc gatcgtcgcc gatatgaacc ggcgcgtgcg ctgtttgccg aaagcctcga
     4501 cctgttcaag tctctcggca atgccgtcca ggtcgccacc ctcaccgccg atctggggtt
     4561 ggtagccatg caacaggccg attacccact ggccgaagcc tgttatcagg aagcgctcag
     4621 caaatatcgt cagaacgaca tcccggaggc tctcagtgac gtgctataca ggttgggtga
     4681 cctctatcgg cttcggaacg acatcgacgg cgcggcggca tattatcagg agagtctgga
     4741 tcggattcga ccgcttaact acaagctcgg catcgcctgc ggactgcaca aactgggaca
     4801 agtggcgcga ttgaccgggc agccgcaaat tgcccgcgat cgcctgcgcg aagcgctcgt
     4861 gctgcagcac gaagttgcca acaagcaagg gatcgtcgag tgtctggcgg cgctgggagg
     4921 tctggcgctg gaagacagcc aaatcgggag ggctgtccag ctgcttggcg cggcggaagc
     4981 gctgctcgcc gagcttgatg cgccgctctc tcccccggat cgcgatcagt tgctgcgcga
     5041 tgtggcggcc gttcgtgaag ccgtcggagc gggcaactat cacgggccgg ttgacacggg
     5101 aaacccgctc tcgtgggagt cggccgtaga gttggggcta caggtgcagt cttcggactg
     5161 acatcaacct tccaggttct cggggtcttt cgtcgccggg cgatgttccg gttcacccgg
     5221 cagccgcgca aacggccgct gctggatgat cgtctgctcc ggcaaacact gcacgaagtt
     5281 gaaggcgggc atggcggcgt ggtcggcgcg aatcatctct tcggtcggcc aggtggtgaa
     5341 gccggcaaag gtgctggcga cggcgcaata gaggataccg tcgcggatgg tctgcatgga
     5401 gttgtggatg tggccgtaga agacgccccg cagccggccg cgggcgggca atagcgcctg
     5461 gtgcatctcc tcgccgttga aaatgagcat gttggcgtcc atccacggcg cgttgaggcg
     5521 cagggcaggg tagtgggtga agacgcacag cggcgggccg tcgggcgtgg cctcggcccg
     5581 cagcacgtcc atctgttgcg gggaaagcag gccgtggggg tcgatctggt ccgggccgcg
     5641 ggcgtccagc accagaaagc gctcgccgcg cacctcgaaa gtataggaga gcgtgtcggt
     5701 tcccggctgg caatcgacct tcggccccat gttcaaatag cgatggatat cggccgagcg
     5761 gtcatggttg ccggtgcaat agtagatggg aatttccagc gcggcgaaag tttcggccgc
     5821 cagccggtag gcggcttggg tcggatgcgt cgtcacatcg ccggtgtgga tcacaaaatc
     5881 aggccgcgtc ggcaggcggt tgagcatatc gatcacgtgc cgcgcgtagg gcagcgaccg
     5941 atggccttgc cgcctgtact cctccgtcgg accgaaatgg gtgtcgctga gatggacgaa
     6001 gtagaccggt tccatgatga cgaaatacga gatacgaaat gcgagatacg agagcgcctc
     6061 caaactcgta tctcgtattt cgtatctcgt atcttgctaa ggtgcgaccg ccaggagcgc
     6121 gctgccgata tcgttgacca gagtgttgac tgtgctgtcc atcaggtgga agtagcccgc
     6181 gggagcgatg ccgatccaga agatgaagac caggaaggcc agcaaaaccg ccagctcgcg
     6241 gcgattcatg ggcggcaggg tgcgtacttc gtcggtcgtc atcggcccca tgaacaggcg
     6301 ctggaagaac cacagcagat aaacggcggc caggatgacg ccggtggtgg cgaaggcggt
     6361 gtagatccac ggatgcgggc caagcgattc cgcgcccatc gagccgagca ggatggtgaa
     6421 ctcgccgacg aagccgttca atcccggcag gcccatgctc gacagcacga tgatgagcga
     6481 cagagcgctg aacaaaggca tgaccgacca gacgccgccg tagaccgaca tctcgcgctt
     6541 gtgggtgcgc tcatagagca tgccgacgac gaagaacagc gcgcccgtgc tcaggccgtg
     6601 gttgacgccc tgcaggatgc cgccctggat gccggcgcta ttgagcgaga agatgcccag
     6661 cgtgacgaag ccaaggtggc tgatactgga gtaggcgacc agcttcttgg cgtccgtctg
     6721 ggcaaaggag acgatggccc cgtagatgat gccgatgatg gccagcacgg cgatgatcgg
     6781 cgcgtagtgg ctggatgcct ccgggaacag cggcagattg aagcgcatga agccgtaggt
     6841 gcccatcttc agcagcacac cggccaggat gatggatccg gccgtgggcg cttcggtgtg
     6901 ggcgtcgggc agccacgaat ggaacgggaa aaccggcacc ttgatggcga aggccacgaa
     6961 gaaggcgatg aacagcaggt tctgggcgtg ggcgaaggcc tcgcgcccgg cgatcaggtc
     7021 gggcagggcg aaggtctggt tggtgatgcc catatagagg atggcgacga gcatcagcaa
     7081 cgacccggcc atcgtgtaga ggaagaactt gatcgacgca tagacacgcc gtgcgccgcc
     7141 ccagatgccg atcaggaaat acatcggcac cagcgtgaac tcccagaaca cgtagaacag
     7201 gaacaggtcc tgggccagga agacgccgag catgcccatc tccagcagca gcatgaagat
     7261 atagtaggcc ttgagctggg tttggatggc gctccatgac ccgatggtgg ccagcagaga
     7321 gatgaacgtc gtcagcagca ccatgaggat gctgaggccg tccacgccca tgaagtagct
     7381 gatgccccat gatgggatcc agtcgacgcg gtcgatgagc tgcagcgccg gattggtcgc
     7441 gtcgaaccgg aacaggacga acagcgacat cgcaaacgtg acggccgagg ccgcaatggc
     7501 gatccacttg atcgtgtcac gattcgagcc gtcttccgac tcttttacga ataatattgc
     7561 cagaacaccg accaggggaa ggaaggtgag tatcgttagc aaccagttca taattcccca
     7621 atctcctaat ttgccatgaa caggaaataa acgagtaatc cgaccgcgcc gaggaacacc
     7681 gccagcgcgt aggtgcgagc gtagccggtt tgcgtcaggc gcaatacccc ggccagccgt
     7741 cgcgtcaccg ctcccgcgcc gttcaccacg ccatcaacgc cttgcgcgtc gaggacattg
     7801 gaagagaagg cggcaaaggt gacgaacata tcgcgaatga ctcggtcatg aatgaagtcg
     7861 tgccagaatt ccccgtcgag cttgaaagcc gaccaggcgg cgaaacgatt gaacagcggg
     7921 accaggcccc gcgaataaaa cgtgttgagc ggcagaatat tccaccacca gatgggcgtg
     7981 cgcgccagcc ggtcggcctc gtcccaggtg cgcggccgcc cgcgatagac cagccaggcc
     8041 agcagcagac cgcccacggc cagcagcatc gacaacccgg caacgaccgg cgagaggaca
     8101 accggcgtat ggggcatatg caccacgcct tcctcggtca aatggatggc cgggatggaa
     8161 tgctcccccc actgctccag caagaaccag aatccgcggg ggtgcatctc gttgcgctcg
     8221 gccatggcct tcgtcagata gggggcgttg agcaagccgc ccagagcggt cagcacggcc
     8281 aggacgatga gtggccgggt cataagcgcg gggctttcgg cggcatgttc agccgcggcg
     8341 tggcggggct tgccgaagaa gaccatgatg agctgccggc cgacatagaa ggccgtgccc
     8401 agcgccgcca gcgccagcag gatgaagatg ggcatctcat gagtctgggc atggagcagg
     8461 atttcgtcct tcgaccagaa gccggacaat ggaaagatgc cggccagcgc cagcgcgccg
     8521 atcatataag tgatataggt cacgcgcatc cggttgcgca gcccgcccat gtagcgcatg
     8581 tcctgtgggt cgaagccgtc gtcttcgtgg tgggcgtcgt ggccgtcgtg accgtcgccg
     8641 tcgtcgccat gaccgtgagc gatctcatga tgcccgtgct ccataccgtg gatgaccgag
     8701 ccggaaccga ggaagagcag cgccttgaag aaagcgtggg tgatcaggtg gaacatggcg
     8761 gcgacatagg cgcccatgcc cgcggcggca accatgaagc cgagctggct gacggtggag
     8821 taggccagca ccttcttgat atcgttctgg ctgaaggcga tcagcccggc gaagaaggcg
     8881 gtcagcgcgc cggtccaggc caccagatcg ggtgtagaga tgaggccgag gatgagcgcg
     8941 ccgctgtcgc gaacgacctc gtagaggaca ttgctgcgca cgagcaggta gacgccggcg
     9001 gtgaccatcg tcgccgcgtg catgagggcg gagacgggcg tggggccggc catggcgtcg
     9061 ggcagccagg tatagagcgg aatctgggcc gatttgccgg ccgcgccaag cagcagcagg
     9121 gcggtgacgg ccgtcagcac ggtggggagc gacagactca tgccaccgaa ctggacgaca
     9181 tgattcgcct tgtacatctc gaccgcgccg tcgaagatcg gcgtgtagtc cagcgtgccg
     9241 aatgtccaga agagcaggaa catcgccagc agaacgccga agtcgccgat gcggttcatg
     9301 accatggctt tacgggcagc gttggagttg gccggcgcgc cgttcttgtc cacgcggcca
     9361 aaccagaagc cgatgagcaa ataggaacac aggcccacgc cctcccagcc tacgaacatc
     9421 atcagatagc tgctcgcgct caccaggata agcatgaaga agacaaacag gttgagatag
     9481 gtgaaaaagc gcgagaagtc ggggtcgtca tgcatatagc cgatggcata gatatggatg
     9541 agcgtgccga tgccggagac gaccagcatc atcgtcaccg aaagtgtgtc cacctgctgc
     9601 gtccagccta cctggaagtt cgcgccgggg atgttgatcc agtccataat cggcacatac
     9661 caggcctcga aaccgttggt cgccaggctg acgagcagcg tgagggagac gacaaacgcc
     9721 ccgaaagtca tcagggtggc cagccagccg acaaaacgct ctccgctctt gcggtttcgg
     9781 tctacgatgc cgcggccgaa caagccgttg aacaacagcc cggccagcgg cagatagaga
     9841 atcaatggag ctaatgagaa tgtattcata cgcgccccgg ggaggaatta tgaattacga
     9901 attatgaatt acgaattcgt cgctcatcat tcgtcgttcg ttatcctctt atccctttaa
     9961 caagttaatt tcgtcgatat tgatgctctt ccgggtgcgg aagatggcca cgatcagcgc
    10021 caggccgacg gcgacctcgg ccgcggcgac ggtgatgacg aaaaagacca gcacctgtcc
    10081 gtccaaatcg cccagatggc gagcgaaggc cacgaacagc aggttggcgc tgttgagcat
    10141 caattcgatt gacatgaata aaatgatggc attgcgacgc aagagcacgc ccaacgcgcc
    10201 gatgacgaac aggatgccgc tcagggcaat gtaccagttg atatctaccg cgctaggcat
    10261 tgtctttccc tcgcttcttg gtatgaccga agatgacgac gccggtgacg gccgccagca
    10321 gcagcacccc ggtcacctgg aacgggaaca cataggattg gaacagggct atccccagag
    10381 cctgcgggcc ggcgtcaatg gacgggctga gcgccgtgct gccgaggctg ttctggaaca
    10441 acagatagcc gaagacgccg atgagaacta cggccagcag caccgccgca tagcgatggt
    10501 agcgctcgct gccacgcacc tcatcggccg cgccgcgcaa tttctccgcg cccaggagca
    10561 tgatgacgaa gagaaacagg atgacgatcg cgcccgcgta cacggtgatc tgcaccatgg
    10621 cgatgaacgg ggcgttcaac accagataca ggacggcaac cgtcatgaag ttgagcacca
    10681 gatagagcgc gctatgcacg gcattgcggc tcagcaacat gcccaacgcg gcggcgacgg
    10741 cgacggccgc cagcaggatg aagagtatcg gatgaatcat atagtcgcct cgaattaatt
    10801 acgaattacg aattacgaat tacgaattag gactgattac cccctcaatt cctaattctt
    10861 aattcctaat tttcttattt cgggttttcc atatccggta tcgcgcgggt gaatgtgccc
    10921 ggctcggtgg cctgcggcgt cgggatgcca ttgaccgggg catcgaccaa cagcatctct
    10981 ttggtataga tcgcgctcgc ccgatcgtag aagctaagct catactggtg ctccagcaca
    11041 atggcttccg tggggcaggc atcctcgcaa tagccgcaga agatgcagcg catcatattg
    11101 atctcgtaga cacgcgcgta gcgctcgccg ggcgaatagc gctcctggtc ggtgttctcg
    11161 gccgcttcca caaaaatggc gtcggccggg caagcggcgg cgcacagcgc gcagccgatg
    11221 cacttctcca gaccattgtc atatctcttt aattcatggc gacccttgaa gcgcggccgc
    11281 accggccgct cgacctccgg gtattggatg gtcaccggct ggatgaacag atgcttcagc
    11341 gtcgtgccca tgcctttgag aatctcacca atcatgcggt tctcctatta taaatggtcg
    11401 gcgctcggca atgggttcca ttgacgaacg gccgccggcc attcaactgc tatccggcaa
    11461 aaaacagctt caccgattcg atcaacgggg tcagcgtgcc ttcctcggcc atgacgatca
    11521 acaccgccgt cacaatgaaa ttgagcacgg cgatgggcag cagggtcttc cagccgaagc
    11581 ccatcagttg atcgtagcgg aagcggggca gcgtggcccg aatccagatc atcacgaaca
    11641 gactggcgat gatcttgcca agaatataga cgatgcccag ccacgggaat tggtcgacaa
    11701 acggtcctct gtagccgccc agaaaaaacg tggcgaaaat ggcgctgagg gagatcatct
    11761 tcatgtattc ggccatgaag aacatgccga agcgcatggc gccgtactcc acgttgaaac
    11821 cggccgacaa ttcctgctcg gcttccagca agtcgaacgg cgcgcgctgc aattcggcca
    11881 gcgcggcaat gtagaacacc agcgccgcca gcggctggat gaatatgtac cagaccggcc
    11941 gctgcgcatc gacgatcacg cccaggtcca tcgaatcggc catcatgatc gggatgagga
    12001 tgatgatgcc cagcgccagc tcgtagctga tcatctgcgc cgaggcacgg atgccgccca
    12061 ggatggcgta cttgctgttc gaggcccacc cggccaggac gacgccgtag acgccgatgg
    12121 acgtgatagc cagaatgaac aacacgccga cgtcgatccc cggcgcaatg gcgaaatagg
    12181 gctggaactt ccacgggcca agccggaagg cgggcgccca ggggatgacg gccaggatca
    12241 ggatggcggg gatgacggcc agcatcgggg ccaggttata gatcacccgg tcgaccttgg
    12301 ccggcgtcgg gtcttccttg aagaacagct tgaccgcatc ggccgcgggc tgcatgaagc
    12361 cgctcaggaa gcgcttctcg ccgccgcgcc acgggatggg gatatacccc gtccggttgg
    12421 ggccatagcg cgcctgcagg cggcccaaca ccttgcgctc catgagcgtg gtgtaggcga
    12481 agccggtgat gacggccagg ctgatgataa agccgaggat caatgcgcgt attgcgattt
    12541 gaccgggagt cataagctac tcctcatgat aaataatgat ctgcctgttg ccggggatga
    12601 tacgggcctt tttcttgcgc cgtttcatcc gtcggttgtt tattactcaa tcccctgtca
    12661 ccatgtcggc cgcgtgccgg cgggtgacga tggccggttc gaaaccggcc ggccatgggg
    12721 cgggcacgcc gcgcagcagg cccagcccgg tgggcaccga ctcgtctaca acggcttcca
    12781 cgtcgtaggt ctcgccgccc atctggacgg tcagaggctc gccgtccatg aagtttacgg
    12841 catcgagcgg gttgaggcac aatgccggtc gggccatgcg cttttccagc aatttcgtgt
    12901 ggttgatgag cgtccccggc gtgtaaaggg ccgggatgcg cagcacccgg aagccgtcgg
    12961 ccgggatgcc atgcggcgct tccacgggca gatcgaagtt ccccacagcg ccggattcgg
    13021 ccgccgcggc ccattgctgc cccaggcctt gcttgttctc gaaggccatg ccgccgtaat
    13081 agaaatcctt gctgccgacg atgggccatt gcgcttcgac gtgggccagg gtgcggtaat
    13141 ccatgccccg ataaggttcc acggacttgg cgatctcctt gaaggccagg ctggccgcaa
    13201 acggcggctt gcccagcccg acgcgctcgc tgagctgcgc caggatttgc cagtccgggc
    13261 gcgcctcgcc tacgaccggg atggccgggt agtagcgctg cacgcgccgc tcgccgctgg
    13321 tgaaggtgcc ttcgcgctcg gcccagctct gggccggcaa gaccacatcg gccagcagcg
    13381 ccgtctcggt caggaacagc tcctggacga cgagaaaggc gagcttgccg cggccgtcca
    13441 tcaggccgtc gcccaccggg tccgcgccca tgacgtagag cgccgacacg cggccgtcgg
    13501 ccgctccggc gtagatctcg gccgcgtcgc ggcccgctgc agcagtcggg gcatagcccg
    13561 gcccgaaggc gggatggata cccatgtccc acgcgccctg agtgttggcg tgggcccaga
    13621 ccggcaccag gccattgttc acccgcccgg cgcgggccgt cgcgtcgtcg ccgtcgcctt
    13681 gcttggcgag taacaggttg cccagcatgc gcgccagtgt gtcggtttca tcgtaagtca
    13741 gcccttccgc gccgtagaaa atgaccagat tcgcggcatt cgcgagcgca ttggcggcgg
    13801 ccgccaggtc gccctcggcg gttccgtcgg ttaacgcgcg cacggccgcc agcgtgccgt
    13861 cgggcgcgta gtggagcagg tggctcgcga acttgtccag ccgggtgggc cgggcattga
    13921 gcacgatcac cgtcgcgccg cggtcggcgg cctgcttgac gcgcagccac cagaccgggg
    13981 tttcctcgtg cagatcggca gccaccacca ggatggcatc gcccttcttt aggtccttca
    14041 ggttgctgcc gcgggccagg cctacctggg cgacgacatc gccgccggcc agattgcgct
    14101 ggaccaaatc aatattgccg ttgcccagcc cctggcggaa caggcgctgg aacaggtagt
    14161 aatcctcgtt gctcaagcgc tcgccggtga tgccggccgc gccgttcttg tgctgttgca
    14221 gtttgccggc cacgtagtcg agcgcgtcgt cccaggacac cggctccagg cggccgtcgc
    14281 ggcgcaccag cggctggcgc agccggtcgg gcgcgtcggc gaagtgatgg acgaagcggc
    14341 ccttgtcgca gatccaaagc tcgttcaccg cctcgttctg gcggggcatg atgcgcttga
    14401 tcacgttgcg gccgcccgcg atcgcctcgc gccgggtgtt cagggtcagg ttgcagccga
    14461 cggggcattg ggtgcagatg ctggcgaccg gcgtcagctc ccacggccgc gcgccaaagc
    14521 ggaagtcggc cgaggtgagc gcgccgacgg ggcagatgtc ggtcgtgttg ccgctccaga
    14581 tgctgtcgaa gccgggcttg gagaaggtca cgatctccag ccgccggccg cgattgtgga
    14641 aatggatgac cggatcatcg acgatctcgg cctggaagcg ggtgcagcgg gcgcactgga
    14701 tgcagcgctc gcggtcgagg acgatcagct cgcccagcgg gacgcgcttg tcaagcttga
    14761 tcttgtcgcg gaagtcgatg cgggcctcgt cgtgaccata ggccatcgtc agctcctgca
    14821 gcgggcactc gccgcccttg tcacagacag ggcaatcgag cgggtggctg gtcagcagga
    14881 attcgaggat atcctcgcgg gcgtgcttga cgaccggggt ctgggtgcgc acgaccatgc
    14941 cctcgcccac ggcgacggtg cagccggtct gcaacccctt gccgaagttc agcttcaccg
    15001 aaccatcggc attgagcact ttctcgcccg tggcgcggtc gatgaccggc atgccgatct
    15061 ccaccaggca catgcggcac atgccgaccg gggccagctt gggatggtag cagaaaaccg
    15121 gaatgtcgat gtcgattttc ttggcggcgt cgacgaccag cgtcccccgt aggacggaaa
    15181 cttcaacgcc atcgatggtc aggtttacca tgttactcat atggtcactc ttgtacttct
    15241 tctgtcgcgc cgattggtcc cagcaagtcg gccatccgca aggccaggcc ggggaacagg
    15301 gtcggcgcga attcgtcggt tgccgcagcc gtctggttga cgagatagcc gtcggccgtg
    15361 tggcggtatt cggtgatgag cagcgtgtcg ctctccacca gccaatacca gggcacgccg
    15421 gcctgatagt aggcgcggag tttctgcgac cggtcgcggg cagccgtgga aggcgaaatc
    15481 acctcaacaa ccagatcggg cggcccctga atagcggtgt cgtcnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnggcggc ccttcgggca ggcgacgata atccgccgcc
    15661 gtccagcgcc gctctataac cattggagtt gccgataggg tcgtcacgcg ttctctatcc
    15721 ctcgttcttg tatcggtcag tggtcggtcg ccacgctagg ccgcaaccgc ctgctttttc
    15781 tttgcgccgg cagccacctt ggccttgtac tccggcagga agtggcgaat cgacgataaa
    15841 atcgggttgg tggcgaattc gcccagcgcg cagaggcatt tgccggtcat attggtggcg
    15901 atgctctcca gcagcttgat gtcgtcctcg gtgccgccgc cgtggtagag acgatcgata
    15961 accttctcca tccagtaggt gccttcgcgg cagggggtac acttaccgca agactcatgg
    16021 cggaagaaat gggtcatctt cagcgcggcc cagaccatgt cggtggtctc gtccatgacg
    16081 atgagcgagg ccgaacccat gaccgaatgg aaaggagtca ggccctcata gctgcagggc
    16141 gcgtcgaggg cgtccgcgac gatgatgggg ccggaaccgc cgctgggcag cagggccttg
    16201 aacttcttgc cgccgcgcac gccgccggcc atgtcgatca actggcgata ggtcgtgccg
    16261 agggggactt cgtagttgcc cggccgctcg acgtggccgc tgaggcagac gatcttcaca
    16321 ccggggctgt cggccgttcc catctgcttg aaccagtccg cgccattgac cagaatggcg
    16381 gccacattgg ccagcgtttc ggtgttgttg acgacggtcg gcttgcgata gagaccctcg
    16441 acggccggga aggggggctt cgaccacggc tggcccagat tgcccgctat cgactcgatc
    16501 agcgccgtct cctcgccgca gatataggct cccgcgccgt agtgggtgta aaactcgacg
    16561 gagtaatcgc tgcccatgat gttcttgccg ataatcccgg cctcgcggca ctccagcagc
    16621 gcttcttcga aggcgtaggc cgcgtccata aactcgccgc gcatatagtt gaagaccaga
    16681 tgggagttga tggcgtaggc ggcgatgagc gcgccttcga tgatctggtg ggggttgtgc
    16741 tcggtcagct cccgatcctt gaacgtgccg gtctccgatt catcggtatt gatcaggaca
    16801 taggtatcgg gcgcgttctt ggggatgaag ctccacttca cgccggtggg gaagcccgcg
    16861 ccgccacggc cgcgcaggtt cgacgccttg acgacatcga tcacctcggc cggggtcatc
    16921 gacagggcct tcttcagccc ctcataaccg ccgtgttggc gatagacttc aagcttgtgc
    16981 aggtccggta tttcgcgatg tcgcaataat acgtgtgcca taatccaaat cctcaatcga
    17041 gatacgaaat acgagatacg aaatacgagt tcagaagcgt gttcttattt cgtattctgt
    17101 gcgtcagacg cggtatagcc aatgatgcgc tcgacgacgg tcggttcggg gtcgccggcg
    17161 gccgctttgg cccgcaaatc ggtcaataat gccccgaatt tcgcctcgtc caggttctca
    17221 tagaacttga ggttgcactg gagcatgggc gcgcggtcgc atgcgccgag gcacatgacg
    17281 gtcttgagcg tgaacatgcc gtcgttggtc gtgccgtcca ccgggatgtc gagcttgcgc
    17341 gaggccatct cgacgaactc atccgcgccg cgcagggcgc aagccaggtc gttgcagact
    17401 tccagcacgt acttgccggt cggttcctca tagaagaggg aatagaaccc ggccagcgac
    17461 aggacgtggg tggggtccag ctcgaggatg tcggccacct cgcgcatggc ctcgtcgctc
    17521 atgtaaccgt actcgttctg cgccaggtgc atcaggtgca ggatggccga tttcttctcc
    17581 gggaagcggg ccagcaaggc gtcaatttct tgggcgtatt tgtctcttaa cacagtgttc
    17641 tccggaaggc ttagccgtga tccttctctc ttatcgatcg atctcgccca ggacgatgtc
    17701 gattgagccg atcgtcgcca caacgtcggc gatgaagcga ttcttcgaca tgagcggcag
    17761 cgaggccaga ttcacgtagg acgggccgcg gaaatgaacg cgggccggct tgttggtccc
    17821 gttgccgcgc aaatagcagc caatctcgcc gcgcggcgac tcgatgacct gataaacata
    17881 tccctcgggg gcgctgaacc cttccgtcca cagcttgaag tggtggatga ccgcttccat
    17941 gctgcggccg agatcggcgc gcggcggcgg gacgatcttg tggtcgtcga tgttgaccgg
    18001 gccgggcttc aggcgatcga ggccctggcg aatgaggcgc aggcattcgc gcatctcgcg
    18061 catgcggcaa cgataccggg cgtagacgtc gccctcgtgc tccaccggca cgtcgaactc
    18121 ataggtctca tagccgcagt aaggttggtc gcggcggttg tcccaatcga cgccggaggc
    18181 gcgcatgctg gggccggtga ggccccaggc aatggcttcg tcgcccgaga tgacgccgac
    18241 gcccttggtg cgcatcagga agatggggtt cttgtcgagc agcgcctcgt agtcggcgat
    18301 cttggccggc atgaagtcga gcagcttgcg cacagccggc tcgaactccg gcggcacgtc
    18361 gcgccacaga ccgccgggac ggaaatagct gaccatcatg cgctggccgg ccaccatctc
    18421 gaaaatgtcc aggatgtatt cgcgctcgcg gaagcagtag agaaacaccg acatggcggc
    18481 caggtcgagc gacgatgtgc ccagccacac cagatggctg gccagacgcg acaactcggc
    18541 caggatgagg cggagcgtct gggcgcgcgg ggtaatctcg atgccgagca gcttctccac
    18601 agccatgatg tagaccatgt tgttggtcat gggggagagg tagtccatgc gatcggtcgc
    18661 ggtcagggcc ttgacgtagc gcttgttctc catggtcttc tcgatgccgg tgtggagaaa
    18721 gccgatatcg ggagccagat tgacgaccgt ctcgccgtcc agctccacca acaggcgcag
    18781 gacgccgtgg gtgctgggat gctgcggccc catgctgagc agcatgtgct cttcggggtc
    18841 gatgatctcc atccccgtgc ccactttggc gcggagctcc tcgacggtga gttcctgaat
    18901 actgtcgctt gcttgcggta ttaaaaaggc catatcaaaa ctccgaagcc ttaatgatca
    18961 gtgatcaata gccgaaaatc actgtttggc ataaggtttc ttgctgtcga tctcttgcca
    19021 gttgaaactg aactgcactt cttcgtagcc caacgggtaa tccttgcgca gcgggtgacc
    19081 ttgccagtcc tcgggcagga aaaggcggcg caggctgttg ttgccggcga agcgcacacc
    19141 catcagatcc cacacttcca tctccagcca ggtggcgatg ggccagatgt cggccgcggt
    19201 cggcgggccg tcgtcggggt cctcgaccca gacgcgcagc cggacgcggt gcttgttggg
    19261 catggaaagc aggtgatagt tgacggcgaa gcgcgggtag tcgggcaggt agtcgtcagc
    19321 cacgatgtcg gacagaaagt tgtatttcag ctcggggtcg tcgcgcatca ggcgacaggc
    19381 gtcgacaagc ttgtcctggc gaatgtgcac cgtgcgctcg ccgcgaaagt cgctgacctg
    19441 ctcaactgcg tcgcccagcg cggcccgcag cttgtccact atcatgtcat cgcgtgtcat
    19501 gtgaaacact ccagaatgac gaatgacgaa ttaggaatta ggaaacggtt gctcttgatt
    19561 cgtaattcgt aattcgtaat ttccattagg cccagtcggt tagtttgtcg ttgcggatct
    19621 tgtcgtgcag cgccatgatg ccgtcaatga gcgcttcggg gcgcggcggg cagccggcca
    19681 cgtagacatc gacggggatg atctcgtcca cgccctggac gatggcgtaa ttattgaata
    19741 caccgccgca cgaggcacag tcgcccatgg cgatgaccca cttggggtag ggcatctgat
    19801 catagaggcg acggatgacg ggggccatct tgcgcgacac gcggccggcg atgatcatca
    19861 ggtccgattg gcgcggcgac ggccgattca gctccatgcc gaagcgggaa aggtcgtagt
    19921 gggcggcctg ggtcgacatc atttcgatgg cgcagcaggc caaaccaaac agcataggcc
    19981 acatggcatt cgtgcggccc caattgacga catcttccag gcgataggtg acaacaccca
    20041 gattgcccgc tttttgttct attcccattc caaaactcct ttcttcatgg atggcgcaag
    20101 ttcaggcgct attcccactc cagtacgcct ttgcgccata cccacaggtc gccgacgatg
    20161 aacatggtca tgaagatcag catcatcgcc agcccaaagt agccgaggtt gcggaaggtg
    20221 accgcccacg gcaccagcaa aataatgtcg acgtcgaaga ggataaacaa cacggcgatg
    20281 cgatagaact tcaccggcag gcggcggttg gcctcgccga tggcggtcat acccgattca
    20341 tagggcgcca gcgcgcgttt gccgagccgc ttcggcccca ggctgaggga caggatgggc
    20401 agaagcaacg cgaaaaagac agcaacgccg aacaagacgg cgatcgggag gtagtcgatg
    20461 agtatttttt ccataatcgc gctcggttgt ttcgggcagg ataacgggat ctcgcagcaa
    20521 ccctggcggg atcatagtcc taccgcgcga gatgcgccaa taaatacaat atatgggaga
    20581 accagagcct ctcccacgat gtgaattcta tcacaagtaa atagcaggcc aaaaaatggt
    20641 tgttaatcgc ggtt
//
LOCUS       DAOMQG010000022        19484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1203_length_19484_cov_10.005353, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000022 DAOMQG010000000
VERSION     DAOMQG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 19484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19484
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1203_length_19484_cov_10.005353"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..>1095)
                     /locus_tag="PLC06_02645"
     CDS             complement(92..>1095)
                     /locus_tag="PLC06_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89373.1"
                     /translation="SLFLFILALTLMKDGARGLAPIVQNRFSLDGPANTMGFGWLFAY
                     IVMSGSPVAASALTFLDAGIIDEIQTFTMIVGSRMGASFIILFIGFIYVLRGRNRSTS
                     LSMGLLSFTVTGTLQAGALVIGIMMLASGILDKIQISKGMAITAITDLIFDPISAFFQ
                     SILPTWALFLVGMGIILITFNLFDRCLPEMAIKESQVGRVSRLVYQPMIMFLLGAAVT
                     LVSMSVSVSLSILVPLSHRGFIRRENVIPYIMGANITTFIDTLLAAVLLNNPAAITVV
                     MAQMLAVAITSLIILLTVFRRYERASLRFVHWVTIKNRNLALFMFAIFIIPLVLVLI"
     assembly_gap    1096..1195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1196..2862)
                     /locus_tag="PLC06_02650"
     CDS             complement(<1196..2862)
                     /locus_tag="PLC06_02650"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRO89374.1"
                     /translation="MTRKWLFGLLCCALFFTTGAQLDKTYRAERFDVEVAVQPDGSLN
                     IEETVVFRFTGGPFSFVFRELPTDHTDGITGITAGVDGVAWPQGTGPGEVEISGRDPI
                     RVEWHLPPTADTSQTFTLAYTQLGVVERGGEADVLNWQALPDEYEYEIDNSRLTINFP
                     ASGRLIGEPEITAGEATVTTESGRAIFDMQNLSPGDPLVVRLSFAPGAFTGAPPAWQA
                     QQEGQSSRAWIWITASIAVLAGGFIALIQAARNNMRSVPKSNSYVQKPPLDLPPALAG
                     YLANQSVSWQHALATLFDLAERGHITIEQIREKSLLRSADFSLTLLDRPDGLRPHESA
                     LLDLLFTDKSGVPHDTITMGKMSHLITSSRWKGYTHTLEDEAEQEGLTNPLVKGKQKK
                     AIGWGVALLILAPVLLIAGALFDSLFGMWPLLLMGAIALIGFLAVIFGASYTKLSDKG
                     FQYATTFEPFRRFLQDVSKNKTDLPDNTYYEAYLPYATGYGLANQWVKRMGESDDPRI
                     PAYFRAAEVDEAVNMASFIAVIMAASNSGGAAGAAAAGAAGAAAAGGG"
     gene            complement(2993..4570)
                     /locus_tag="PLC06_02655"
     CDS             complement(2993..4570)
                     /locus_tag="PLC06_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRO89375.1"
                     /translation="MNDKFDAIVIGAGHNGLVAAATLAMAGKRVLVVEKRDVIGGAAA
                     TEEIFPGHHVDTGGADASLLQEEIIQRLNLQQHGLQFHEGPALLFAPQPDGRALTIWR
                     DDARTAAEITRFSARDAAQWPAFRRQVERFAGMLREMLLLTPPDLAGLQRGDLMSWGP
                     LALRIRRQGGEDMMELFRVLPMAVQAYLDEWFESDALKGALGGSAVTGTALGPRSGGT
                     NLMFLYQNLGGLLNHRFITGGIGRLSESLAAVAKAHDATIRTGAGVEQILIENGAAVG
                     VRLESGEEIRSRVVMSNADPRRTLFGMVGPQHLEPNVMRSVRNIIYRGTTARLNLAVN
                     GLPAFAGQPNEEQLSGRIRLSPSLDYLERAYDASKYGRCSEHPYLEIFIPTIRDRSLI
                     TGPQAKNGHHILTATMQYAPYALREGGWPTAAEALTTTFLDALETIAPGIRRQIVHQR
                     LTTPLDYECDYHLTEGSIYHGQMGLDQMLVMRPIPEWSRYETPIRNLYLCGAGAHPGG
                     GITGAPGYNAARALLHS"
     assembly_gap    4597..4696
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4814..6403)
                     /locus_tag="PLC06_02660"
     CDS             complement(4814..6403)
                     /locus_tag="PLC06_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRO89376.1"
                     /translation="MNTNQYDAIVIGGGHNGLVAAGYLARAGKKTLVLEKRYLVGGAS
                     VTEEVFPGFKFTVFSYVVSLFRPEIIRDLNLPAHGLTILPLESTLTPTYDGRDCLYRD
                     GDHYQTLRNIARFSQRDADAYDEYARYMYFMAKAVRYILGVIPPDPTTFNPKDLKGLL
                     DMARHFLGLSQDQLYVLAKLMTMSAADFVEQWFESEPLKATLSASGIIGTFLGPRSPG
                     TAYVLLHHYMGEIDGAYRAWGFHKGGTGGLAQVLARAAEAQGAEIRCNAGVAQVLTKG
                     GRTSGVVLENGDEIHAPLVLSSLDPKQTFLRLVDPNQLPDDLVGAVRRFQTLGSSGKV
                     NLALDRAPELASRPGVGRHLAGAISMSPNLDYIERAYDDAKYGNFSREPYVDVIIPSM
                     IDPDMAPPGKHVMSCFVQYAPYKLADGEWTDEKREAFGDAVVNVLERAFPDIRDIILH
                     RQVLTPVDVERITGITQGNIFHGELNLSQLFFLRPAAGYAKYRTPIKGYYQCGSGTHP
                     GGGIMGAPGRLGALEALKDAR"
     gene            complement(6444..7643)
                     /locus_tag="PLC06_02665"
     CDS             complement(6444..7643)
                     /locus_tag="PLC06_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HRO89377.1"
                     /translation="MPLPTPFHSRTSAVCDSYEWRNWSGFLSAGIYEMSHDREYYAIR
                     NSAALIDVTPLFKYEVSGPDAARLVDRVITRDVTRQRVGQVYYTPWCDSRGKVIDDGT
                     VSRLDTNHFRITSADPSGRWFQDVGYGLNATVTNVTADLAALAIQGPRSRAILKEVVG
                     GIDFDQLKYFHLAQGTVDGFPITVTRTGYTGDLGYELWVQPEYAERLWDCLVDRGARH
                     GILPAGIIALDIARVEAGLIMLAVDYVSSDHAVIESQKSSPYEIGLGWAVSPSKGGFI
                     GRQALMTEREKGSTWGFAGIQYDWNALEKLFAEEDLPPHVAGRASRSAVPVYEAGNPG
                     HQIGQITSHTFSPIMKQYIGIGTLLNPHAQIGNRVDVEVTVEFKRKLCPAIIVPTPFF
                     DPPRKKS"
     gene            complement(7685..8506)
                     /locus_tag="PLC06_02670"
     CDS             complement(7685..8506)
                     /locus_tag="PLC06_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRO89378.1"
                     /translation="MSATTEPMAGTQSVRRAVAMLEAFTDERPSWTVSDLVEHTGLNR
                     TTAYRLLTALESAEYVHRDPVGDSYRLGSGLIALGGRAQRAYTVRTIALPELEALATR
                     SGETATLEIMHDGKMVTIEEIPGEYVTSGNQHIGSRWPIHATSTGKAMLAHLPMREVE
                     VLLSESLPSLTEKTITSAAHLRAQLAKIRDDGYAVAAEELELGFVAVGAPLLDANGLP
                     VAAISLGGTLTRMTAERIPQFGTEIRTAAARISHRLGYRQQSEPTNGTDEKQSHL"
     gene            complement(8503..10020)
                     /locus_tag="PLC06_02675"
     CDS             complement(8503..10020)
                     /locus_tag="PLC06_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HRO89379.1"
                     /translation="MQPRIQVLDNTLIEQIVTESRRVLVEVGMEVRGLELRQQLLAAG
                     LPAGKDGRILFPPDVIDQALATAPHSFTLYDRDGNAHAELGGDKVHFTPASSGLKMLD
                     HRTGETRSANTADFAEYVRLADGLNNIAYLATAFSTNDIEAVVSDAWRLYLCLTNSRK
                     PVVSGAFTEHGVGRMAEMMQLFRHDRADLIARPMSIFTITATGMFRYSEDSCQNLLDC
                     VEWGIPVEIVPVTLMGLIAPVSLVGATIFHTVDTLAGIIMAQIIKPGAPVLFGGAPAE
                     FHMRAATSPMLGIQALHLDVAYAAVGKALGLPTQAYMALSDSKFLDAQAGAETFGSAL
                     LAALAGINSVSGPGMLDYVLTFSLPKLVYDDDLCGQALHFVREIAPVGDLPITDLVRE
                     LMAEQHLITAEHTLTHWSRELYMPSSITDRDNRENWAKAGGKDLMQRATDEVERRLAA
                     YEPLETDPLIVAEMERIITSDMSEPAPLPDVPAAHHQAGDAAGEGDRRRHRRFAR"
     gene            complement(10157..11098)
                     /locus_tag="PLC06_02680"
     CDS             complement(10157..11098)
                     /locus_tag="PLC06_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRO89380.1"
                     /translation="MTTTQNNGSWKFRTLERVRQLRVKRRNEAAGRIIRDYLLIILGS
                     VLLAISMHLFFIPHRLVAGGLSGTAQLINNFTGWPIGTVSFVLNIPLFALGWFYLGGH
                     RFLARTVFASFAFAVILDGLQFLWPAVVITDDLTLNTLYGGVTAGVAVGLIFRARATS
                     GGTDILARILERKFGTPLSQAYLYTDGLVVFLAGLAFGWERALYAVIALYVGGVLVEV
                     TLNGSNVMRVATIITTEPQAVADRILADMERGVTEWNGRGMYTGTERHVLLCAVSRAD
                     TVMLKAIIHEADPHAFVIIGQAKEVYGEGFRSLKENA"
     gene            complement(11095..11907)
                     /locus_tag="PLC06_02685"
     CDS             complement(11095..11907)
                     /locus_tag="PLC06_02685"
                     /inference="COORDINATES: protein motif:HMM:NF039315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HRO89381.1"
                     /translation="MTQFPSLDGFGPTRRTLQLYAQALSSLARAHAPAHPNWWHISLK
                     VTPLGLVTGNIPCGGGGILVGQMDFHRACISLIGSDGRSWSIPMDDGLSGSSMGERVL
                     AAATEAGISGEVDRSRFADDRPGVYDPDAVRRFFTAVVRAQRVFTRHRARLGIDAGPV
                     QLWPHGFDLSMEWFGTRVESYKEGDKTLELPAQLNLGFYPGDDDAGSYFYSSPWPFDS
                     DKLLNHELPGAAVWHTEGWQGAMLPYAAVDDEDKLLNFAGAVFDIASPLLTK"
     gene            complement(11966..12547)
                     /gene="ruvA"
                     /locus_tag="PLC06_02690"
     CDS             complement(11966..12547)
                     /gene="ruvA"
                     /locus_tag="PLC06_02690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRO89382.1"
                     /translation="MIASLSGTVQKIEPNSLVIHMGGVGLRVFVPRTVLEDVGGVGRR
                     LTLHTHLQVREDNLSLFGFESEEDLQLFEVLLDVNGVGPKVALAILGTLSPELLKSAI
                     MREETAVLQRVPGIGKKTAERIMFHLRDKLDLTGAAEVLPLVSDVDADVIDILTGLGF
                     SIVEAQSALQHVPREVSAIDERVGAALQYLDQI"
     gene            complement(12575..13081)
                     /gene="ruvC"
                     /locus_tag="PLC06_02695"
     CDS             complement(12575..13081)
                     /gene="ruvC"
                     /locus_tag="PLC06_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRO89383.1"
                     /translation="MRILGLDPGTATTGYGIIDVVAGELKLVEYGVIATAAGEPLERR
                     LQSIFRQLNTLIATYRPDRAAVEQLFFGRNITTAIAVGQARGVLLLALADAAIPMSEY
                     SPPKVKEAVTGYGKAEKAQMQLMVRHLLNLEESPRPDDAADALAVAITHARYVAFDMV
                     IDGSGLHS"
     gene            complement(13105..13854)
                     /locus_tag="PLC06_02700"
     CDS             complement(13105..13854)
                     /locus_tag="PLC06_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRO89384.1"
                     /translation="MSGHSKWSTIKHKKAASDAKRGKVFTRIAKEITIAAREGGGDPS
                     SNTGLRLAVAKAKEANMPKDNIEKAIKRGTGEIEGGELVDIVYEAYGPHSVGILIEVV
                     TDNRNRAIADVRHAVSKYGGSMAEAGAVSWQFKRKGYISIIDRVDQDELFMVAAEAGA
                     DDIQFNDDITEIYCELESFRAVQEALEAAGYKMDDFSLIYDPANRIELSQSASMQVLN
                     LIEKIEDLDDVQNVYSALEMSDEVLAAMEAA"
     gene            complement(14028..15386)
                     /locus_tag="PLC06_02705"
     CDS             complement(14028..15386)
                     /locus_tag="PLC06_02705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRO89385.1"
                     /translation="MAHATMSFPPDFLWGTATSSYQVEGNNTNSDWYAWEAADPTRIK
                     GDLGCGQACDWWNNAEAYLDIAAEMGNNAHRMSLEWSRIEPEPSVFDPEAIARYRAIL
                     QACHDRGLEPMVTLHHFSNPIWLVEKGDFNSEIVVDYFRRYVAKVVAELGDLIPKWVT
                     INEPITYGVLRYLNHEFPSGKRTGIGALFEAIHNMLRCHAAAYHTIKESRPDALVGTA
                     NNMQVFEPRAGGSAIDRFWVNRIDRLYNDSWQDALHTGRYHGIIGSKRIKHLAGTYDF
                     VGINYYTRFYLRFPPPPGFVDREWGPDAIVSDGQYGEIYPRGLFIMLNRVWKRYGKPV
                     YVTENGVPDAVDRIRPGFLLSHLQQIWYAVNFCYPVMGYYHWSLVDNLEWDRGWTQKF
                     GLIEVDPITQERSWRPSAYLYQEICRSYSISDDMARRYAPEMLPIMWPGTEPISSDLP
                     VR"
     gene            15576..16211
                     /locus_tag="PLC06_02710"
     CDS             15576..16211
                     /locus_tag="PLC06_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HRO89386.1"
                     /translation="MKNIVPLSGYYLSNRLGRVYIDSLEETIGRNGKNALLNLTGCGA
                     FIDTPPSDDLALGFDMAVVSNLNRAMEVIYGPRGARGLALRSGRAMYDRLADAFDIPI
                     GFNELAFRLLPLQTRLKIGVPALARVFTQHSDQTHRVADHGTYFDYIVERCAVCWGQE
                     TKTPLCHLIVGLLQEALSAFSRGQEFRVTQTECRAVGAAACVFRIEKEPIV"
     gene            16208..>19484
                     /locus_tag="PLC06_02715"
     CDS             16208..>19484
                     /locus_tag="PLC06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRO89387.1"
                     /translation="MIRDRLRALRPDIAIALAYLILPLLLYAGVTIGPRTMLPADNLF
                     QWPPWREAAAEFDATIPHNALIGDLIIQNYAWKRFVLTSLKGGEIPLWNPYLFAGAPF
                     LANGQHAALYPFSLLFLVIPLAKAYGWFALSQVWLAGISMYLFSRILGLRRGSAALAG
                     FVYQGAQFLIISAAVFPMIAAAVVWLPLLLGCIERVITTLFGLSERHHRVVLWAAAGS
                     IALGCQILAGHAEFTYYTLLVMGLYALWRVGTQAWIARDESRVANRESGVATSDISLV
                     VRRSSLAFLWLAAMVGTGLLIGAIQLVPLFEVGRINFRAGSASFEEVRSWAYPPRNAI
                     TFLLPDFFGNPTDHAVRDPFSGELVPLERNSFGELNPYGAFTTSWGTKNYVEGAAYMG
                     ILPLLLAGLGVWALFRRRGLSALQRARMWFFAGLSFFSLAFIFGTPLYALLFYGLPFI
                     NQLHTPFRWVFPLALSVSVLAGFGAERLPGAEISAGEQGIRGAGESARSEWRSQWRPQ
                     GFALVPLLLGGAVLIGTMASWLFHDAFRPLIERLFLGLAGATYAFPSAEIFYGYLFWQ
                     LLAFGGLLLASGVVLLLARRTRYWIVAACGLIVLDLFFASLGFNAATDPALLDYTPAA
                     VRWLKEQPGEWRLTTYDTIGAGTLNANTPWHHDLMDIRGYDSIIPKQYTDYMVAIEPQ
                     NGLQFNRIQPLGSPTAVESPLLDALAVKYIVSAETLDLPKLRPVWAGEGVTIYENLAV
                     VPRAFALPATAEVHVPDALDSLSQYDPRYYVVVESDTPLPAAAEPGVPLPASIANASN
                     NEIIATVQVAEPSWLILSDSYFSGWRAYVRPAGAADDAESEVEIVRVNANFRGVRLEP
                     GEWTVRYRYSPRSFQLGGLMSFMGGILILFAAGVWGWRRFYRPESTTSTTRSVAKNSA
                     APMVLNLFNKAIDFVFAAFYLRLLGPADAGSFATAIATAGIFEIVANYGLNILLIRDV
                     SQDRSLAGRFLLNSSVLRIFTALIGALPIAAYMFVASQGTNPLSSAETTAIIFMMIGM
                     VLSGMALGVSGLFYVYEEAEVPAAMSTVTTILKVGLGVVVLLAGYSFVGLAGVSIVVN
                     LITL"
BASE COUNT         3640 a         5604 c         6014 g         4026 t
ORIGIN      
        1 gattggtgaa atattaacat acttatcggt tgcctgagta ggggattacg ccccctgtcg
       61 ggcaatttgt cgtaagttca tgccctgatc cctagatgag aaccaggacc agcgggatga
      121 taaatatcgc aaacatgaat aatgccagat tgcggttctt tattgtcacc caatggacaa
      181 accggaggga ggcacgttca tagcgacgga ataccgtgag caggatgatg agtgacgtga
      241 tggcgacggc cagcatctgg gccatgacga ctgtgatggc tgcaggatta ttgagcaaca
      301 cggcggccag caaggtgtcg atgaacgtcg tgatgttggc ccccatgata taggggatga
      361 cattttcccg acggataaac ccgcgatggc tgagcgggac caggatgctg agcgaaacac
      421 tcaccgacat cgagaccagt gtgaccgccg cgcccaacag gaacatgatc atcggctgat
      481 agaccaaacg cgaaacgcgg cctacctggc tttccttgat ggccatttcg ggcagacatc
      541 tgtcaaacag gttaaaggtg atcaggataa tgcccatgcc gacgaggaac aacgcccacg
      601 ttggcaatat gctctgaaag aacgcggaga tcggatcgaa gatcagatcg gtgatggcgg
      661 taattgccat tcccttgctg atctgtatct tgtcgagaat gccggaagcc agcatcatga
      721 taccgatgac cagcgcccct gcctgaagcg tgcccgtgac cgtaaatgac agcaacccca
      781 tgctgaggct ggtcgatcga ttgcggccgc gcagcacata gatgaagccg atgaacagga
      841 tgatgaagct ggcgcccatt cgactgccga cgatcatcgt gaaggtctgg atttcgtcga
      901 taatgcctgc atccaggaag gtgagggccg aggcggcgac gggtgatccg ctcatgacaa
      961 tataagcgaa gagccagcca aatcccatgg tattggccgg gccatccagt gagaatcgat
     1021 tctgaacgat cggcgccaag ccgcgagcgc catccttcat caaggtcagg gccaggatga
     1081 acaggaacag gctcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgcc
     1201 gccggctgcg gcggcgcctg ctgcccccgc ggccgcggcc cccgccgccc cgcccgaatt
     1261 ggatgcggcc atgatgacgg cgataaacga ggccatattg accgcctcgt ctacctcggc
     1321 agcacggaag taggccggaa tgcgcgggtc atcagactcg cccatccgtt tgacccactg
     1381 gttggccaat ccgtagccgg tggcgtaggg cagataggct tcatagtagg tgttatccgg
     1441 caggtcggtc ttgtttttgg acacgtcctg cagaaatcgc cggaaaggct cgaaggtggt
     1501 ggcgtactgg aaacctttat ccgacagttt ggtatatgac gcgccgaaga tcaccgccag
     1561 aaaaccgatg agggcaatgg cccccatcag cagcagaggc cacataccaa agagggaatc
     1621 gaacagggcg ccggcaatga gcagcaccgg ggccaggatc aggagcgcca ctccccatcc
     1681 gatggctttt ttctgttttc ctttaacgag tgggtttgtt aacccttctt gttcggcttc
     1741 gtcctccagc gtgtgcgtgt atcccttcca acgtgaggac gtgatgaggt ggctcatttt
     1801 gcccatggtg atggtgtcgt gcgggacgcc gctcttgtcg gtgaaaagca ggtcgagcag
     1861 ggcagactca tgcggtcgta gcccatcggg acgatccagc agcgtcagac tgaagtcggc
     1921 cgagcgcagc aaggacttct cgcgtatctg ctcgatcgtg atgtggccgc gctctgccag
     1981 atcaaacagg gttgccagcg cgtgttgcca ggataccgac tggttggcca agtatccggc
     2041 cagcgcggga ggcaggtcga gcggcggctt ctgcacatag gagttggact tgggtacgga
     2101 acgcatatta ttgcgcgcgg cctgtatcag cgcgatgaat ccgccggcca gaacggctat
     2161 ggaggcagtg atccatatcc aggcccggct gctttggccc tcctgttgcg cttgccaggc
     2221 aggcggcgcg ccggtgaaag ctcccggtgc gaacgacagg cgcacgacga gcgggtcgcc
     2281 cggtgacagg ttttgcatgt cgaagatagc gcggccggat tcggtggtga cggtggcttc
     2341 gccggccgtg atttcaggct cgccaatcag ccgtcccgac gcgggaaagt tgatggtcag
     2401 acggctgttg tcgatctcat attcatactc atcgggcaag gcctgccaat tgaggacgtc
     2461 ggcctctccc ccgcgttcga caacgccaag ctgggtatac gccagggtga atgtttgcga
     2521 cgtgtcggcg gtcgggggca gatgccactc taccctgatg ggatcgcggc cgctgatttc
     2581 gacctcgccc ggcccggttc cctgaggcca ggcgacgcca tccaccccgg ccgtgatgcc
     2641 ggtgatgccg tcggtgtggt cggtgggtaa ttcgcgaaag acgaaggaga aaggcccgcc
     2701 ggtgaaacgg aaaacgaccg tttcttcgat gttgagcgat ccgtccggct gcacggcgac
     2761 ttcgacgtcg aaccgttcgg cccgataggt tttgtcaagt tgagcgcccg ttgtgaagaa
     2821 tagagcgcag cacagcagcc cgaataacca ttttcgagtc atcgataacc tccttgttat
     2881 cttcatgcca ttcataaccc tttacggttg cacgaatgat tggttgcgcg aagacgggcg
     2941 cgacgggttg gggccgggag gacgcccgca acccgtcgtc tgaggcggaa catcacgagt
     3001 gcagaagagc tcgtgcggcg ttgtagccgg gcgcgccggt gatgccacct ccgggatgag
     3061 cgcccgcacc gcatagatag aggttcctga tcggcgtctc gtagcggctc cattcgggga
     3121 tggggcgcat taccagcatc tgatccagcc ccatttgtcc gtggtagatg ctgccctcag
     3181 tcagatgata atcgcattca tagtccaggg gtgtggtcag gcgttgatgg acgatttgcc
     3241 ggcgaatacc cggagcgatt gtctccaggg cgtctaaaaa ggtcgttgtc agggcctcgg
     3301 ccgcagtggg ccaaccaccc tctcgcagcg cgtaaggcgc gtattgcatc gtagcggtga
     3361 ggatgtggtg gccgtttttt gcttgtgggc cggtgatgag cgagcggtcg cggatggtcg
     3421 ggatgaagat ttccagatag gggtgttcgg agcagcggcc gtatttcgag gcgtcatagg
     3481 cgcgctccag ataatccagg ctggggctga ggcgaatacg gccgctcagc tgttcttcgt
     3541 tgggttgccc cgcgaatgcc ggcagtccgt tcacggcaag gttgagccgc gccgtcgtgc
     3601 cgcgatagat gatattgcgc acgctgcgca tcacattcgg ctcaagatgt tgcggcccga
     3661 ccatgccgaa tagcgtgcgc cgcgggtcgg cgttggacat gacgacgcgg gagcgaattt
     3721 cttcgccgct ctcaaggcga acgccgacgg ccgcgccatt ctcaatcagg atttgctcca
     3781 caccggcacc ggtgcgaatc gttgcatcat gggccttggc gacagcggcc aacgactcag
     3841 acagccggcc gatcccgccg gtgatgaaac gatgattgag tagaccgccg aggttttgat
     3901 agaggaacat caggtttgtt ccgccgctgc gtgggccaag cgccgtgccg gtcacggccg
     3961 aaccacccag tgcgcctttc agggcgtcac tctcaaacca ttcgtcgagg taagcctgga
     4021 cggccatcgg caacacgcgg aacaactcca tcatgtcctc gccgccctgc cgcctgattc
     4081 gcaatgccag cggcccccac gacatgaggt cgccgcgttg gagaccagcc agatcgggcg
     4141 gcgtcaggag cagcatttcg cgcagcatgc cggcaaagcg ttctacctgc cggcggaatg
     4201 cgggccattg agcggcgtcg cgcgcgctga acctggtgat ttcggctgcc gtccgcgcat
     4261 cgtcacgcca gatagtgagc gcgcggccgt ccggttgggg cgcgaagagc aaggctgggc
     4321 cttcgtggaa ctgcaagcca tgctgctgca aattcagccg ttggatgatc tcctcttgca
     4381 ataacgaagc gtcggctccg ccggtatcca catgatggcc gggaaaaatc tcttcggtag
     4441 cggccgctcc gccgattacg tcgcgcttct ccaccaccag cacacgcttg ccggccatgg
     4501 ccagcgtcgc cgcagcgacc agcccattgt gccccgcgcc gatcacgatt gcgtcaaact
     4561 tgtcgttcat cgaaattact ctcttgctcc ggaatgnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnatca cgattgcgtc aaacttgtcg ttcatcgaaa ttactctctt
     4741 gctccggaat gccgggacaa atgcgatatc tcctcgctcc tctgccccct cgctcccttt
     4801 cgccctttca caattaccgc gcatccttca acgcttccag cgcgcccaac cgccccggcg
     4861 cgcccatgat gccgccgccg ggatgtgtgc ctgacccgca ttgatagtag cccttgattg
     4921 gcgtgcgata cttggcatag cccgcggccg gacgcaggaa gaacaactgg gaaagattta
     4981 gctcgccatg gaagatattg ccctgcgtaa tgcccgttat ccgctccacg tcgaccggcg
     5041 taaggacctg ccgatggaga atgatgtccc tgatatccgg gaaggcgcgc tccagcacat
     5101 tgacgacagc gtcgccaaac gcctcgcgct tctcgtcggt ccactcgcca tcagcgagct
     5161 tatagggcgc gtattgcacg aagcaggaca tgacgtgctt gccgggcggg gccatgtcgg
     5221 ggtcgatcat cgaggggatg atgacgtcca cgtagggttc gcgagagaag ttgccgtatt
     5281 tggcgtcgtc gtaggcgcgc tcgatgtagt cgaggttagg actcattgag atcgccccgg
     5341 ccagatggcg gccgacgccg ggtcggcttg ccagctccgg cgcgcgatcc agcgccagat
     5401 tgaccttgcc cgacgagcca agcgtctgga agcggcgcac ggcgccaacc agatcgtcgg
     5461 gcaactggtt ggggtcgacc aatctgagga aggtctgctt cgggtcaagg ctggacagca
     5521 ccagaggggc gtgaatctca tcaccgtttt ccagaacgac gccagacgtt cggccgccct
     5581 ttgtcagcac ctgggccaca ccggcattgc agcgaatctc ggccccttgt gcctcggcgg
     5641 cgcgggccaa tacctgcgcc aacccaccgg tgccgccttt gtggaagccc caggcgcggt
     5701 aagcgccgtc gatctcgccc atgtagtgat ggagcaacac gtaggccgtg cccggtgagc
     5761 gcgggccgag gaaggtgccg atgatgccgc tggccgagag ggtggccttt agtggctcgg
     5821 actcgaacca ttgctcgacg aaatcggccg cgctcatcgt catcagcttg gccagcacgt
     5881 aaagctgatc ctggctgaga cccaggaaat gacgagccat gtcaagcagc cccttgagat
     5941 ctttggggtt aaacgtcgtc ggatcgggcg ggatgacgcc caggatatag cgcacggcct
     6001 tggccatgaa gtacatgtag cgggcgtact cgtcgtaggc gtcggcatca cgctgtgaga
     6061 agcgggcaat gttgcgcagc gtctggtaat ggtcgccgtc gcgatagaga caatcgcggc
     6121 cgtcgtaggt cggggttaga gtactctcca gcggcagaat cgtcagccca tgggccggga
     6181 gattcagatc gcggatgatc tccggccgga aaaggctgac cacgtaggag aagacggtga
     6241 acttgaagcc ggggaaaact tcttcagtga cggacgcgcc gccgaccaga tagcgtttct
     6301 ccagcaccag cgtcttcttg cccgcccgcg ccagataacc tgcggcgaca agaccgttgt
     6361 gtccgccgcc gatgacgata gcatcgtatt ggtttgtgtt cataaaatat gcgttccttg
     6421 ggtgcaggga agatcgcttg cctttagctt ttctttcgcg gtggatcgaa gaacggtgtg
     6481 ggcacgatga ttgccgggca aagcttgcgt ttgaactcca ccgtcacctc gacgtcgacc
     6541 cgattaccga tttgagcgtg cgggttcagc agcgtgccga tgccgatata ttgcttcatg
     6601 atgggggaga aggtgtggct ggtgatttgg ccaatctggt gacctggatt gcccgcctcg
     6661 tagaccggca cggctgaacg agacgcccgc cccgccacgt gtggcggcag atcttcttcg
     6721 gcaaacagct tctccagagc attccaatcg tactggatgc ccgcgaagcc ccaggtcgat
     6781 cccttttcgc gttcggtcat cagcgcttgg cgcccgatga aacccccttt gctcggcgac
     6841 acagcccagc ccaacccaat ttcatagggc gatgacttct ggctctcgat aacggcatgg
     6901 tcgctggaga cgtaatcgac ggccagcatg attaaccccg cctccacgcg ggcgatgtcg
     6961 agggcgatga tgcccgcggg caagatgcca tgcctcgccc cccggtcaac gaggcagtcc
     7021 cacagccgtt cagcgtattc gggctgtacc catagctcat aacccaaatc gcctgtgtac
     7081 ccggtgcggg tgacggtaat ggggaagcca tcgaccgtgc cctgggccag atggaagtat
     7141 ttcaattgat cgaagtcgat gccgccaaca acttccttca gaatcgcccg gctgcgcggt
     7201 ccctggatgg ccaaagcggc cagatcggcg gtcacgttag tgacggtggc atttagccca
     7261 tagcccacgt cctgaaacca gcggccgctc ggatcggccg aagtgatgcg aaaatggttt
     7321 gtgtccagcc gggagacagt tccgtcgtcg atcaccttgc cgcgcgaatc gcaccacggc
     7381 gtgtagtaga cctgtccgac gcgctgtctg gtcacgtcgc gggtgatgac tcgatcaact
     7441 agtcgcgccg cgtcggggcc ggatacctcg tatttgaaca aaggcgtcac gtcgatcagc
     7501 gccgcgctgt tgcgaatggc gtaatactcg cggtcgtggg acatctcata gatgcccgcc
     7561 gacaggaagc ccgaccagtt gcgccattcg tagctgtcgc agacggccga cgtccggcta
     7621 tgaaaaggtg tcggtagcgg catattcttt ctccaataag ggcgatccgc agattgtgcg
     7681 gatgttatag atggctctgt ttttcatctg tgccatttgt gggttctgat tgttgtctgt
     7741 agccgaggcg gtgggagatg cgagcggcag cggtgcgtat ctccgtgcca aattgcggga
     7801 tgcgttcggc ggtcatgcgc gtgagtgtgc cgccgaggct gatcgcagcc acagggaggc
     7861 cgttggcgtc cagaagcggt gcgccgacgg cgacgaagcc caattccagc tcttcggccg
     7921 ccacggcgta gccgtcatcg cgaatcttcg ccagttgcgc gcgaagatga gcggctgagg
     7981 tgatcgtctt ttccgtcaac gacggtagcg actcggacaa taatacttcc acttcccgca
     8041 tgggcaggtg cgccagcatc gctttaccgg tcgaggtagc atggattggc cagcggctgc
     8101 cgatgtgctg attgccgctg gtcacgtact cgccggggat ttcttcgatg gtcaccatct
     8161 tgccgtcatg cataatttcc agggtggccg tctcgcccga acgcgtagcc agcgcttcaa
     8221 gttccggcaa ggcgatggtg cgcacggtgt aggctcgttg ggcgcggccg cccagcgcga
     8281 tgaggcctga gccaagacga tagctgtcgc cgacgggatc gcgatggacg tattcggcgc
     8341 tctccagcgc cgtcagcagc cggtaggccg tcgtccggtt gaggccggtg tgttcgacca
     8401 aatcgctgac ggtccatgac ggccgctcgt cggtgaaagc ctccagcatg gccacggcgc
     8461 gccgaacgga ttgtgtgccg gccatcggct cggttgtcgc gctcatcggg cgaacctccg
     8521 gtgacgacga cgatccccct cgccggccgc gtccccagcc tgatgatgcg cggcggggac
     8581 atccggcagc ggcgcgggtt cgctcatgtc cgacgtgatg atccgttcca tttcggccac
     8641 gatgagcgga tcggtttcca gcggttcata ggccgccagc cgtcgctcga cctcgtccgt
     8701 ggctcgctgc atcaaatcct tgccgccggc cttggcccag ttctcccgat tgtcacgatc
     8761 ggtgatcgac gagggcatat acagctcacg cgaccagtgg gtcaaagtat gttcggcggt
     8821 gatcagatgt tgttcggcca tcaattcacg aacgagatcg gtgatcggca gatcgcccac
     8881 aggcgctatt tcacgcacga aatgcaatgc ctggccgcac agatcgtcat catagaccag
     8941 cttgggcaga ctgaaggtga gtacgtaatc gagcatgccc ggcccggaga cggagttgat
     9001 gcccgccagc gccgccagca aggcgctgcc gaacgtctcc gcgccggcct gagcgtcaag
     9061 aaacttgctg tcgctcaggg ccatgtaggc ctgcgtgggc agccccagcg ccttgccgac
     9121 ggctgcatag gccacgtcga gatgtaaggc ctggatgccc aacatgggcg aagtggcggc
     9181 tctcatgtga aactcggccg gcgcgccacc gaagagcacc ggtgcgcccg gcttgatgat
     9241 ctgggccata atgatgccgg cgagtgtgtc tacggtatgg aagatagtgg ccccgacaag
     9301 cgagaccggc gcgatcagcc ccattagcgt taccggcacg atctcgaccg ggatgcccca
     9361 ctcgacacaa tcgagcaggt tttggcagga gtcctcacta tagcggaaca tgcccgtggc
     9421 ggtgatggtg aagatcgaca tcggccgcgc gatcagatcg gcccggtcgt gacggaaaag
     9481 ctgcatcatt tcggccatgc ggccgacgcc atgttcggtg aacgcgccgg agacgactgg
     9541 tttgcgcgag ttggtgaggc acagatagag tcgccaggcg tcggagacca ccgcttcgat
     9601 gtcgttggtc gagaaggcgg tggccaagta ggcgatgtta ttgaggccgt cagccaggcg
     9661 gacgtactcg gcaaagtcgg ccgtgttcgc cgagcgcgtc tccccggtgc gatggtcgag
     9721 cattttcaga ccactggaag ccggggtaaa gtggactttg tcgcctccca actcggcatg
     9781 ggcgttgccg tcacgatcgt acagggtgaa ggagtgtggg gcggtggcga gcgcctggtc
     9841 aattacgtcg ggcgggaaga ggatgcgacc gtctttgccg gcgggcagac cggcagccag
     9901 cagttgctgc cgcagctcca gcccacgcac ctccatgccg acctcgacca ggacgcgccg
     9961 tgattcagtc acgatttgct cgatcagtgt gttgtccaga acctgaatgc gcggctgcat
    10021 gatgcctcca tcttgttcac aatgtgaaca tatcgttcat ggaagtgtaa cggattgatg
    10081 gggggatgca aggagagaaa aggggaaggg gagaaagggg gaaagggagc agggacgggg
    10141 gagaattgag ttgttgtcag gcgttttctt tcaggctgcg gaagccttcg ccgtatactt
    10201 ccttggcctg gccgatgatc acgaaggcgt gggggtcagc ctcatggata atggccttga
    10261 gcatcaccgt gtcggcgcga ctcacggcgc acagcaggac atggcgttcc gtgccggtat
    10321 acatgccgcg gccgttccac tctgtgacgc cgcgttccat atcggccaga atacgatcgg
    10381 ccacggcttg cggttcggtg gtgatgatcg ttgccacgcg catgacgttg gagccgttga
    10441 gcgtcacctc taccagcacg ccacccacgt agagcgcgat gacggcgtag agtgctcgct
    10501 cccaaccgaa ggccagcccg gccaaaaaga cgacgaggcc gtcggtgtag aggtaagcct
    10561 gggatagcgg cgtgccgaat ttgcgctcca ggatgcgggc cagaatgtcg gttccaccgc
    10621 tggtagctcg agcgcggaag atcaagccga cggcgacccc ggccgtcacg ccgccataga
    10681 gggtgttgag agtcaggtcg tcggtgatga cgacggcggg ccaaaggaat tgcagtccgt
    10741 cgagaataac cgcgaacgcg aacgaggcga agacggtccg cgccaaaaac cgatggccgc
    10801 cgagatagaa ccagccgagg gcgaacagcg ggatattgag gacgaacgac accgtgccga
    10861 tgggccaacc ggtgaaattg ttgataagtt gcgccgtgcc gctcagccct ccggcgacga
    10921 gtcggtgagg gatgaagaag agatgcatgc tgatcgccag caacacgctg cccaggatga
    10981 tgagcaaata atcgcgaata attcgcccgg ctgcttcgtt ccgccgtttc acgcgcaatt
    11041 gccgcacccg ctccagtgtc cgaaatttcc agctaccgtt gttttgtgtt gtggtcattt
    11101 ggttaacagc ggcgaggcga tgtcaaatac agctccggca aaattgagca gcttatcttc
    11161 gtcatcaacg gccgcgtagg ggagcattgc tccctgccag ccctcggtgt gccagacggc
    11221 cgcgccgggc aattcgtgat tgagcagctt gtcgctgtca aacggccacg ggctggagta
    11281 gaagtaggag ccggcatcat catcgccggg atagaagccg aggttgagct gggcgggcag
    11341 ctccagcgtc ttgtcaccct ctttgtagct ctccacacgg gtgccgaacc actccatcga
    11401 gaggtcgaag ccgtggggcc aaagctgcac cggtcccgcg tcgatgccca gccgggcgcg
    11461 gtgtcgagtg aatacgcgtt gggcgcggac gacggccgtg aaaaagcgcc ggacggcatc
    11521 gggatcataa acacccggcc tgtcatcggc gaagcgagag cggtcgactt cgccggagat
    11581 acccgcctcg gtcgccgcgg ccagcacgcg ctcgcccata ctgctgccgc ttagaccgtc
    11641 gtccatcggg atcgaccacg agcggccgtc gctgccgatg agcgagatac aggcgcgatg
    11701 gaaatccatc tgcccgacca gaatgccgcc gccgccacaa ggaatgttac ctgtgacaag
    11761 gccaagcggt gtcaccttga ggctgatgtg ccaccagttg gggtgcgccg gggcatgggc
    11821 acgggccagg ctgctcagcg cctgggcgta tagctgcagc gtgcggcgtg ttgggccgaa
    11881 gccgtcaaga gagggaaatt gggtcaagtt gaagctccct tgggcttatg aggcgcgaaa
    11941 ctatgcctca aaggcctgga acgactcaaa tctgatcgag atattgcaac gctgctccca
    12001 cccgttcgtc gatggccgac acctcgcggg ggacgtgctg cagggcggat tgggcttcca
    12061 cgatgctgaa ccccagtccg gtcaggatgt cgattacgtc tgcgtccaca tcactgacca
    12121 gcggcagcac ctcggccgcg ccggtcaggt ccagcttgtc gcgcagatga aacatgatgc
    12181 gttcagcggt ctttttgccg atacccggta cgcgctgaag cacggccgtc tcctcacgca
    12241 tgatggcact tttcagcagc tcggggctga gcgtgccgag gatcgccaga gccactttcg
    12301 ggccaacgcc gttgacatcg agcaagacct cgaagagttg caggtcttcc tcgctctcga
    12361 agccgaagag ggaaaggttg tcctcgcgga cttgcaaatg ggtgtgcagc gtcaggcggc
    12421 ggccgacacc ccccacgtct tccagcaccg tgcgaggcac gaacacccgc aggccaacgc
    12481 cgcccatatg gatgacgagg ctgttgggtt cgatcttctg aacggttccg ctgagcgagg
    12541 cgatcatgag gagttacgag ttacgagcga cgagttacga gtgcaagccg cttccatcta
    12601 tcaccatatc aaaggcgacg tagcgggcgt gggtgatggc gacggcgagc gcatcggccg
    12661 cgtcgtcggg gcgaggcgat tcttccagat tcagcaggtg gcgcaccatg agctgcatct
    12721 gggctttttc ggccttgcca taaccggtga cggcttcctt cacctttggc ggggaatact
    12781 cgctcatcgg gatggcagcg tcggccagcg ccaacagcaa tacgcctcgc gcctgcccca
    12841 cggcgatggc ggtggtgata ttgcgaccga agaataactg ttccacggct gctcgatccg
    12901 gtcgataggt ggcgatcaat gtattgagtt gtcgaaagat ggattgcaac cgtcgctcga
    12961 gcggctcgcc cgcggccgtc gcaatgaccc cgtactccac gagcttcaat tcgccggcta
    13021 cgacgtcgat gatgccgtag ccggtggtgg cggtgccggg gtcaaggccg aggatgcgca
    13081 tggataattc aaaacctcag caggttaagc ggcttccatt gccgccagaa cttcgtcgct
    13141 catctccagg gccgaataga cgttctgaac gtcgtcgagg tcttcgatct tctcgatgag
    13201 attcagcacc tgcattgagg cagattggct cagttcgatg cgattggccg gatcgtagat
    13261 gagcgagaaa tcatccatct tgtagccggc ggcctccagc gcttcctgga cggcgcggaa
    13321 ggactccagc tcacaataga tctcggtgat gtcgtcgttg aactggatgt catcggctcc
    13381 ggcttcggcc gcgaccatga acaactcatc ctgatccacc cgatcgatga tgctgatgta
    13441 gcccttacgc ttgaactgcc aggacaccgc tccggcttcg gccatgctgc cgccatattt
    13501 gctgacggcg tggcgcacgt cggcgatggc gcgattgcga ttgtcggtca cgacctcgat
    13561 gaggatgccg acgctatgtg gcccgtaggc ctcatagacg atatcgacta gctcgccgcc
    13621 ctcgatctcg ccggtgccgc gcttgatagc cttctcgatg ttgtccttgg gcatgttggc
    13681 ttccttggcc ttggccacag ccagccgcag tcccgtattg gacgacggat cgccgccacc
    13741 ttcgcgcgcg gcgatggtga tctctttggc gattcgggtg aagaccttgc cgcgcttcgc
    13801 gtcactggcg gccttcttgt gtttaatggt ggaccatttt gaatgtcctg acatcggttg
    13861 tctccatgat aagggtgccc cgggcggtgc cgcgggaaac ggccgccgcg ccgggcggcc
    13921 gagggtttcg attaaatagc gagtgaatta taacatggaa tgggcaagtt ggggagttgc
    13981 ccattgagag tttgataagt tgatgatgtg ccggccagag gcgaaggtca gcggacaggc
    14041 agatctgaac tgatcggctc cgtgccgggc cacataatcg ggagcatctc cggcgcgtat
    14101 cgtctggcca tgtcatcgct gatgctgtaa ctacgacaaa tctcctgata gagataggcg
    14161 cttggccgcc atgaccgctc ttgggtgatc gggtcaactt caatcaatcc aaacttctgc
    14221 gtccaaccgc gatcccactc caagttgtcg actaatgacc aatggtaata acccatcacc
    14281 ggatagcaaa agttgaccgc gtaccagatt tgctggagat ggctcagcag gaaccccggc
    14341 cggattcgat cgactgcgtc cggcacgccg ttttccgtca cgtaaacagg cttgccgtac
    14401 cgtttccaga cacggttgag catgatgaac aggccacgcg gataaatctc tccatactgc
    14461 ccatcactga cgatggcgtc cggcccccat tcacgatcca cgaatcccgg tggcggtggg
    14521 aaccgcaggt agaagcgggt gtagtaattg ataccgacaa agtcatatgt gccggccaga
    14581 tgtttgatgc gcttgctgcc gatgatcccg tgatagcgtc cggtatggag cgcgtcttgc
    14641 cagctatcgt tgtagagccg gtcgattcga ttgacccaaa agcgatcgat ggcgctgccc
    14701 ccggcgcgtg gctcaaagac ttgcatatta ttggctgtgc ctaccagtgc atccggccgg
    14761 ctttccttga tggtgtgata agcggccgcg tggcaacgca gcatgttgtg aattgcctca
    14821 aataatgccc caatgccggt ccgcttcccg ctcgggaact catggttcag gtagcgcagg
    14881 acgccatagg tgatcggctc attgatggtg acccatttgg ggatgagatc gcccaattcg
    14941 gcgacgactt tggccacgta acgccggaag tagtcgacga cgatttcgct attgaagtcg
    15001 cccttttcaa ccagccatat cggattgctg aaatggtgca gcgttaccat cggctccagg
    15061 ccgcgatcgt ggcaggcctg caggatagca cgatagcggg cgatcgcttc cgggtcgaag
    15121 acgctgggct ccggctcaat gcgactccac tcaagcgaca tcctgtgggc gttgttgccc
    15181 atttcggcgg cgatatcgag ataggcttcg gcgttgttcc accagtcgca agcttgcccg
    15241 caacccagat cgcccttgat ccgcgttggg tcggccgctt cccaagcgta ccaatcgcta
    15301 ttcgtgttat tgccttcgac ctgataggaa gatgtggcgg tgccccataa aaagtccggt
    15361 ggaaaactca tcgtcgcgtg tgccataggt tccattatag cggagcatgc cgaagaaggg
    15421 gaatgagggc aatgagccgg agagttgcgc cgtggatata ctcgcccatc tattgcccta
    15481 tgaagccgcg atcggcttgc atccgcttcc catccgtcta tacttggctt ccgttcggcc
    15541 ccgagccggc agtctgtcat gcccggatat ttgttgtgaa aaacatcgtt cccctcagcg
    15601 gctattattt gtctaaccgg ctcggccgcg tgtatatcga ctcgttggaa gagacgatcg
    15661 gccgcaacgg gaaaaatgcc ttgctgaacc tgaccgggtg cggcgcgttt attgataccc
    15721 cgccatccga tgatttggcg ttgggcttcg atatggctgt ggtctccaac cttaatcggg
    15781 ccatggaagt tatctatggg ccacggggtg ctcgggggct ggccttgcgc agtggccggg
    15841 ccatgtacga tcgccttgcc gacgcttttg atattccgat tggatttaat gagctggcct
    15901 tccgtctgtt gccgttgcag actcgcctca agattggtgt gcctgcgctg gcgcgggtct
    15961 tcacccaaca cagcgaccag acccatcgcg tggccgacca cggaacctat ttcgactaca
    16021 ttgtagagcg ttgcgcggtt tgttgggggc aggaaaccaa aacgccatta tgccatttga
    16081 tcgtgggttt gctccaggaa gcgttgagcg cgtttagccg tggacaggag ttccgcgtga
    16141 cacagaccga atgtcgggcg gttggcgcgg cggcgtgcgt cttccgcatc gaaaaagaac
    16201 ccattgtatg atccgtgatc gccttcgcgc cctccggccg gacatcgcca tcgctctggc
    16261 ttatctgatc ctgccgttgc ttctttatgc gggagtcacg attggcccgc gaacgatgct
    16321 cccggccgat aatctgtttc aatggccccc ctggcgcgag gccgcggccg aatttgacgc
    16381 gacgattccc cacaatgcgc tcatcggcga tctcatcatc cagaattacg cctggaagcg
    16441 atttgtgctg acgagcctca aagggggcga gataccccta tggaaccctt atttgtttgc
    16501 cggagcgcct tttctggcca atggccaaca cgctgcgctc tacccgttca gcctgttgtt
    16561 tctcgttatt ccactggcca aggcctatgg ctggtttgcg ttgagtcagg tatggctggc
    16621 gggcatcagc atgtatctgt tcagccgaat cctcggtctg cggcgaggaa gcgcggcgct
    16681 ggcgggcttt gtctatcaag gcgcgcagtt cctgatcatc agcgcggccg tcttcccaat
    16741 gatcgccgcg gccgtggttt ggctgccgct tctgttaggc tgtattgagc gggtcattac
    16801 cacactcttc ggcttgtcgg aacgccatca tcgcgtcgtg ttatgggcgg ccgcgggtag
    16861 catcgctctg ggatgtcaga ttcttgccgg gcatgccgag ttcacctatt acaccctgct
    16921 cgtcatgggt ctatacgctt tgtggcgtgt gggaacgcaa gcctggatcg cccgtgatga
    16981 atcgcgagtg gcgaaccggg agtcgggcgt agccacgtcc gatatctcgc ttgtcgtccg
    17041 gcgctcgtcc ctggcttttc tctggctcgc cgccatggtc ggcacgggcc tgctcattgg
    17101 cgcgatccag ctagtgccgc tgtttgaagt agggcggatt aattttcggg ccggttccgc
    17161 gtcattcgaa gaggtacgga gttgggccta cccgccgcgc aacgccatca catttctgct
    17221 gcccgatttc ttcggcaatc cgaccgatca cgccgtccgc gatcctttct ccggcgagct
    17281 ggttccgctt gaacggaact ccttcggcga gctaaatccc tacggcgcat ttacgacgag
    17341 ttgggggacg aagaactacg ttgagggggc ggcctacatg ggcatcctgc ctttgctgct
    17401 ggcaggcctg ggtgtttggg cgctgtttcg ccggcgcgga ctctcagcac tccagcgcgc
    17461 gcggatgtgg ttttttgccg ggctgtcgtt tttttctctg gcttttatct ttggcacacc
    17521 tctctacgct ctgcttttct atgggttgcc attcatcaat caactccata caccctttcg
    17581 gtgggttttt ccgctggcgc tgtcggtgtc ggtgttggcc ggttttggcg cggagcgttt
    17641 gccaggggcc gagataagcg caggggagca ggggatcagg ggagcagggg aatctgcccg
    17701 ttccgaatgg cgatcgcaat ggcgcccaca ggggtttgct ttggttccgt tgttattagg
    17761 tggcgctgtc ctgatcggaa ctatggcgag ttggctgttc catgatgcgt ttcggccgct
    17821 aatcgagcgg ttgttcctgg ggttggccgg cgcgacttat gctttcccgt cggccgaaat
    17881 cttctacgga tacctgtttt ggcaattgct ggcgtttggg gggctgctcc tggcgtcggg
    17941 ggttgtcctt cttttggcgc gccgcacgcg ctattggatt gtcgcggctt gcgggttaat
    18001 cgttctggac ctttttttcg ccagtctggg attcaacgcc gccaccgatc cggcgctgct
    18061 ggattacacg ccggccgctg tccgttggct caaggagcag cccggcgaat ggcggctgac
    18121 gacctatgat acgattgggg cgggcacact caacgccaac actccctggc accacgatct
    18181 gatggacatt cgcggctacg attccatcat ccccaaacaa tacaccgatt acatggtcgc
    18241 cattgagccg caaaacgggc ttcagttcaa tcgcattcag cctcttggct ccccgacggc
    18301 cgtcgagtcg cccttgctgg acgcgctggc tgtgaagtac atcgtttcgg ccgagacatt
    18361 ggacttgccc aagctccggc cggtgtgggc gggcgagggc gtgactatct acgaaaatct
    18421 ggccgtcgtg ccgcgcgcct ttgcgctgcc cgccacggct gaagtccacg tccccgacgc
    18481 tctggattcc ctatcacaat acgatccgcg ctactatgtt gtggtggagt cggacacgcc
    18541 tctcccagct gcggccgagc ctggcgtccc cctgccggcg tcgatcgcca acgcgtcgaa
    18601 caatgaaatc atcgcgacag ttcaggtcgc cgagccgtcg tggctgatcc tgagcgactc
    18661 ctatttctcc ggttggcgcg cctacgtgcg gccggccggc gcggccgacg atgccgaaag
    18721 cgaagtggag atcgtccggg tgaacgccaa tttccgcggc gtgcggctgg aaccgggcga
    18781 gtggacggtt cgttatcgct acagcccgcg cagcttccag cttggtggcc ttatgtcgtt
    18841 catgggcggc atcctgattc tgttcgccgc cggcgtatgg ggatggcgtc gtttctatcg
    18901 ccctgaatcg accacttcaa cgacgcgcag cgtcgccaag aacagcgccg cgccgatggt
    18961 tttgaacctg tttaacaagg ccattgactt cgtgttcgcg gcgttttacc tgcgcttgct
    19021 tggcccggcc gacgcaggca gctttgccac ggccatagcg acggccggca tcttcgagat
    19081 cgtggccaac tatggcctca atatcctgct tattcgcgat gtctctcagg atcgcagtct
    19141 ggccggccgc ttcctgctga attccagtgt gctgcggata ttcacggctc tgatcggtgc
    19201 gctgccgatc gcagcctata tgttcgtcgc gtcacagggg acgaacccgc tctcctcggc
    19261 cgagaccacc gccatcattt tcatgatgat cggcatggtg ctctccggca tggcgctggg
    19321 cgtttccggt ctgttctacg tctatgaaga ggccgaggtg cccgcggcga tgtcgaccgt
    19381 gacgacgatt ctgaaggttg ggttgggagt ggttgtgctg ttggccggat acagtttcgt
    19441 ggggctggcc ggggtgtcga tcgtcgttaa tctgatcacg ctgg
//
LOCUS       DAOMQG010000023        19115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1241_length_19115_cov_6.695331, whole genome shotgun sequence.
ACCESSION   DAOMQG010000023 DAOMQG010000000
VERSION     DAOMQG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 19115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19115
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1241_length_19115_cov_6.695331"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..567
                     /locus_tag="PLC06_02720"
     CDS             55..567
                     /locus_tag="PLC06_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89388.1"
                     /translation="MARQDKVVVLAVLFVFAALIGAACSNSRSERAPEQDGRVPFDFL
                     SQPFNEQEAIAFIGSPLPAGATDVHVAGESALDTIVVARFDAPQDDVTAWLAELGITE
                     SLEPGYSPFYSSGPSLSEAADWWEPATADSTEVDYSGLYQQVGAKYYSVVVTPLENGL
                     VRVHLLVHNT"
     gene            783..>1402
                     /locus_tag="PLC06_02725"
     CDS             783..>1402
                     /locus_tag="PLC06_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89389.1"
                     /translation="MQWVSNERYVDAEGKERRGLVARISVGNDALEQSDTLEVYEGMV
                     FPIGQQRYQVVKLKPNRSLSVAPGSSNGYIVIGQLADDSTGAPEMGGMGLTAGTVERL
                     FEDGSAHVYRQDGGETIDLFCHVVEGTWCYFVPQGQIEPYDGLYDPLIEDEARRITSD
                     VAEQLTKELGVETIAQSRNNGVKLRCARPEDALSQVTCRVDRGGGW"
     assembly_gap    1403..1502
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1503..1807
                     /locus_tag="PLC06_02730"
     CDS             <1503..1807
                     /locus_tag="PLC06_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89390.1"
                     /translation="IYQAMGQLIVDAMPEAWLSATACAEIEEDDNGLTWGRYVPAAAP
                     DQQRDFDPDYRCYLMFDELRRRTRKPGHALWVKARFTLQPDGHFDLDFTYPDDGSE"
     gene            1825..2310
                     /locus_tag="PLC06_02735"
     CDS             1825..2310
                     /locus_tag="PLC06_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89391.1"
                     /translation="MTGSDTLCAQFTAHVGSCFGKMFEQRSFEPADCLCLRDGRECIA
                     LYRSPAASLLAELSDGAFHILLGSADAPFPGGTYIDHAGQSGWYALFLLVEFRSGQQI
                     HTKKVLKRFLDGELDPYRFEADLFERWADRILPMFETTQEQSWRAEFRRYFGYPDREN
                     R"
     gene            2483..3052
                     /locus_tag="PLC06_02740"
     CDS             2483..3052
                     /locus_tag="PLC06_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89392.1"
                     /translation="MNTDGFLALLEASAPSSWEVLMAEYEGPLPAACDERYHNVCQAY
                     RQLSPPERCEAEERMSTEQRDWMRAVPDRMAMLSVRLQAPAYLEDALIGAFMNFPVAV
                     TDPLEFQGYRGILAICRRCAQLLGLGPEAFEGQMIYICRDDARRDVREFLMGAASDQQ
                     PARWGFREETGDHGIVFRWGQLPIPEAWL"
     gene            complement(3161..3412)
                     /locus_tag="PLC06_02745"
     CDS             complement(3161..3412)
                     /locus_tag="PLC06_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HRO89393.1"
                     /translation="MTLQQITIEIPEKVLLAEKTDAESFGREILILAAVKLFEIGRLS
                     SGRASELAGMSRIEFLLNLNRYKVFPFASELDDLENAHA"
     gene            complement(3638..4624)
                     /locus_tag="PLC06_02750"
     CDS             complement(3638..4624)
                     /locus_tag="PLC06_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89394.1"
                     /translation="MQISIRVVNNDALVINADVLVLKHAQALFGVDRKVVHRLSKLHA
                     NLDSSLPEYGKFLQVDSFGTIGVKEVLFVGVEQLFEFRYQNVREFAHRALAYLAKNSP
                     QVETICFTLHGGGYGLDEVEAFESEIAGIVDALSKGEFPKGLKEIIIVELHKSLAEQL
                     SQSLKDLFPQGFIVINKGNLAETSSSSSSRLKEAGLSSETKPLVFVAMPFDEKMSDVF
                     HYGIRGAVNNAGYLCERVDQTHFTGDVMEYVKLRIRQADLVLADLTQANANVYLEVGY
                     AWGCEKQTVLLVQDANELKFDVKSQRCLIYKSIRELEEMLHRELEKLPLKAK"
     gene            5037..5600
                     /locus_tag="PLC06_02755"
     CDS             5037..5600
                     /locus_tag="PLC06_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89395.1"
                     /translation="MLTYYDRAVGIVCLETDVFEYEADLSKLLGLLPASPLIWQWSST
                     RRYPVHPRYAGLKLPAHRDWTRLTSLSLDLLAADLVEQDLNDDRRQALLIGSDRELIG
                     TLSNTEEARSVLEDFAAERPPVRMAVAQLGDSGSSFIFMPRHPSRSVQHLLEGWGVSI
                     ASARKRLPYGQLHLARFEALFGLRTAP"
     gene            5656..6384
                     /locus_tag="PLC06_02760"
     CDS             5656..6384
                     /locus_tag="PLC06_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89396.1"
                     /translation="MTEPRSFSQRIFGHSLPADLTEAVGRNTHVPLLGEGWFVAVFVW
                     GGAVAAFTFVFFGSVFFSGMPHANPAAARLTLWLSLFIGLIGGVAGLVVYRRLRRYRS
                     PGGTEADLHRAVALHRRLVAGLLLLLGLAGLWHAWLFSPVTRILGSLSFIMALALGFV
                     ALPSLLALAAGQSRRFGRAAQTLDRLLLLGIGLTGLLVAAPPIARAEPGAFFLLLTQV
                     LGLIALPWVGSMLAAARIHSRMKS"
     gene            complement(6394..6594)
                     /locus_tag="PLC06_02765"
     CDS             complement(6394..6594)
                     /locus_tag="PLC06_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89397.1"
                     /translation="MNTVNLTIPEGPRNGKVFRLAKLGMPKPRKPDERGDLCVTVAAE
                     LPAKLSEEEKKIVREWQALRNK"
     gene            6634..7959
                     /locus_tag="PLC06_02770"
     CDS             6634..7959
                     /locus_tag="PLC06_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRO89398.1"
                     /translation="MMPGAARIRALFEREQIEGLATDHATARGADDLLRGEGWVAAGR
                     VTLEPSGDDLLWAEYPVSRRAPVLTTVSLPGLWATCSCAATRFPCRHIIALLLSDLNE
                     PLPTDADAPDWLPPAAHGWNLTPAVGETQSAERQAAVVAGMADLRLWLSDLAREGLAD
                     LPRRGRAKFLGAANRLEDAYAVEAARALRETAAIPGSGMDWPERLLPDLGRLALLCES
                     FRRLDALSPGERGDTLAAAGFLSPATGERVTDTWRILGQVVEVENRVTHLRSWLRGRA
                     TGRWARLDQAIPAGQLQGVCLPTGALVGGALAFSPSAYPLMARPVGPLAPVHGETGDP
                     AAHDDIDAVMSGWSAALALNPWLRRFPMAIRDVWVEPPLTAGGRWRLRDRAGHVLPLP
                     PQFGHGWSLTALSAGRPMTLFGEWDGALFAPASVLAGTWRPLSAWKVVV"
     gene            7956..9491
                     /locus_tag="PLC06_02775"
     CDS             7956..9491
                     /locus_tag="PLC06_02775"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HRO89399.1"
                     /translation="MSLPPALVTAALVGTGRSGLPAAADLPPFLAALRAEMAARPPEE
                     TLLLLAGAGAFHEAAGRLPERVPAAEWHLPAYRAEGDLRPCAPAAERLLERMLADRDV
                     EFLPELLALLEKAGARAPDRLLPEVLARGVKIPRLRPSLLPVIGERGRWLAALNPAWR
                     YAAVDLADFEQLRAVWNDDPGGRPYLATAVRRRDPAAARRLIESTWRAESEIIRRDLL
                     PVLETGLSMADEPFLERALDDRDAHVRRRAADLLAHLPESRLVARMTAAAGDILSLHD
                     DVLKPSAQRPVTDAMVRDGANRDKEPDGVRTATEWSTLLLRTVGAIPLTHWEARFGLS
                     PEALVAASLAGKWPRTLLNAFATAALRQRDMRWIDPLLEGDQYTERTGMLVATLTPEA
                     CYARLATLIDAGKDESVSVFLRRWPGVWDESSGRALLALFARYCADPVDARLNLTLRF
                     LSRQFAQRCPPSLAQEAADLLQGRAVNRAWEASLNYLLRTLALRRELWGATIGERSPE
                     END"
     gene            9492..10580
                     /locus_tag="PLC06_02780"
     CDS             9492..10580
                     /locus_tag="PLC06_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO89400.1"
                     /translation="MTVSTIQRELAEQAYGDELAALMQADRRPKPPNWRMSPWAVATY
                     ILGGTLDDGTVIAPKYIGSSRLVEIAIATLTTDRALLLIGVPGTGKTWLSEHLAAAIS
                     GDSTLLVQGTAGTSEEVIRYGWNYASLIARGPSAEALVPSPILNAMRGGRVARVEELT
                     RIPSDVQDALLTILSEKLLPVPELGEAVQAVKGFNVIATANDRDRGINELSSALKRRF
                     NTVILPLPATADEEVTIVTSRVAELSRALELPAEQPAAEQIRRVVTIFRELRSGLTED
                     GRAKIKSPSGTLSAGEAISVVNSGLALAAHFGDGRLAADDLAASLTGAVVKDPVQDAL
                     VWQEYLETVMREREGWGDLYSACRELAR"
     gene            10577..12862
                     /locus_tag="PLC06_02785"
     CDS             10577..12862
                     /locus_tag="PLC06_02785"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HRO89401.1"
                     /translation="MSEAELRVFGVRHHGPGTARALLAGLAAYEPDCVLVEGAPDADA
                     VISWLAHPALVMPVALLVYRPDEPQRATFYPFATFSPEYRALRFALERDIAVGFMDLP
                     RRHALALPYGPTMPPAAPFRAIAARTGYGSYEAWWNAAIEQTAGGPEIFPAILELAAG
                     MRSAVEPSSPPEDESPEDRMARQREAAMRQRIREAAASGHRRIAAVCGAWHGPALLNA
                     LETDGAADGALLADLPSVEVESTWVPWTYGRLAQTSGYGAGIASPGWYDHLWAAGEAG
                     LPAAEMSALWLSRVAELLRHEGMDTSPGHVIEAVRLAEALAALRGRPFPTLPELEEAT
                     LSVMCDGGQEPLELIRRRLIVGERMGIVPPDVPVVPLQRDLNAQQARVRLWPEPEKSA
                     LTLDLRQANDLDRSRLLHRLWLLEITWGTPVKTRGAQAGTYAEVWQLQWLPDLSAKVI
                     EAAMWGNTVRDAAAARVNDNVARHTDLPKLVALIDQVISADLPEALPAVLARIEELAA
                     LSRDVGGLLAALPPLADTLRYGGLRQTAEHLPILHHVFDRLLTRACLGLPGACRSLDE
                     NAANEMIERLSAAASAVRLVGNEQAAERWQAALARLADLPDIHPTVAGRATRLLFDEA
                     AIRPRGVEFMMARVERALTPRGSAGVTRFAADWLDGFLRDSGLLLVHDKALWGAIDHW
                     LTGMGEAQFTGILPLLRRTFSSYPEAIREQLQSRAQGGRQRQASGEVPQARFDPERAA
                     AVVPVLRRMLGLPQPPKETAL"
     gene            12859..14013
                     /locus_tag="PLC06_02790"
     CDS             12859..14013
                     /locus_tag="PLC06_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009190234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO89402.1"
                     /translation="MSESAERGRRWQLVLGATEQPDNNEGGDDSAEGGGARRQQQQLS
                     PADRALDEALGALYGDSRGGDLSRSMPDVARWLGDIRAYFPEDVVDVMQRDALKRLDP
                     RRLVEEPELLAGIEPDAALAAKLLSLRKVMPPQTQETARQVVARVVDDLVRRIEYPLR
                     QAVSGRLNRAAHTHRPRKAQEIDWGRTIHANLKHYRPELGTVIPEKLVGFGRRRATLK
                     DVILLVDTSGSMATSVVYSGIIAAVLASLPSINTRLVMFDTAVVDLTDRLDDPVEMLF
                     GLRLGGGTNIDQALSYARGAITRPRDTIVVLISDLFEGGNKERMVRQVQHMLADGVQF
                     ITLLALNDQGAARYNAAVAQELADLGVPAFACTPEQFADLMARALNEQWR"
     gene            14150..14383
                     /locus_tag="PLC06_02795"
     CDS             14150..14383
                     /locus_tag="PLC06_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRO89403.1"
                     /translation="MPTVLREGKYRFTFFSNENREPSHIHVKAGADQAKFWLDPIELA
                     SNYGFKAHELNEIERIIYRHQPDLLEDWYEYFS"
     gene            14367..14666
                     /locus_tag="PLC06_02800"
     CDS             14367..14666
                     /locus_tag="PLC06_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRO89404.1"
                     /translation="MNTLAETQTATKHQFRRAHVPQSALAKSVEFSDDLMHVLLTDGR
                     IISVPVIWFPLLHEATAEQRVNYEIGGGGVSLHWPDIDEDLSVAGLLAGADWQAS"
     gene            complement(14697..15710)
                     /locus_tag="PLC06_02805"
     CDS             complement(14697..15710)
                     /locus_tag="PLC06_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRO89405.1"
                     /translation="MDYKDYYKTLGVKKDAGADEIKKAFRKLARKHHPDVNQGDKAAE
                     EKFKDINEAYEVLSDEEKRAKYDQFGAEWQQFSGAGGQAQDFNWGAWGGRPGAGGGQY
                     RTVSPEEFEQMFGGGVHGGSGFSDFFETLFGGMGARSAGRSRGGFGGSFSSADDPFGG
                     MGARPAPQRLDQEHPIQVTLEEAFSGTTRTLQWEGGKRVDAKIPRGVRTGSKVRLSGQ
                     AGGADGRAGDLYLKVEVLPNAIFERDGDDLRMTQPVDIFTVLLGGKISVPAIDKTVNL
                     TIPEGTRNGKVFRLAKLGMPKLRKPDERGDLYVTVAVELPAKLSEEEKKIVREWREIR
                     GGK"
     gene            complement(15838..16551)
                     /locus_tag="PLC06_02810"
     CDS             complement(15838..16551)
                     /locus_tag="PLC06_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRO89406.1"
                     /translation="MARLIRWNNPYPIWLDRDPVEMMDRMLGDVGRNMVDWNGGRSWN
                     LPLDVMEKDAAYVIHASIPGIDPDALDITLTDNVLTIHGETRREDDEKDEDRYMMRER
                     RFGSFTRSITFPMPIEANKIEASCVNGELILTLPKAEAVRPHRIAVNKTHGRKTLEGQ
                     ARGSNGNGRSHGFGEGQADLPVSAEPKSEGWAEGQAKLPPEAEPKSEGWAEGQAKLPP
                     EAEPKSRGWAEGQATRDRA"
     gene            16933..18996
                     /locus_tag="PLC06_02815"
     CDS             16933..18996
                     /locus_tag="PLC06_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRO89407.1"
                     /translation="MSVLTAAELSHAYGAAELFHDLDLTVEARDRVGLVGPNGVGKTT
                     LLLALAGLLEPLSGRVDQAAGLTIGYLRQEAVLTFAGRDNSVYQEMLTAFADLRRREA
                     DLQAMEAAMADDYSDELLATYGALQEAFEREGGYQYQVEIKRVLLGLGFGQAEWDTPL
                     LHLSGGQKTRVLLGRLLLEKPALLILDEPTNHLDMAAIEWLEGTLRRWDGALIIVSHD
                     RYFLDRVVNRMWELSPDGNNSAELRAWRGNYSAFVAQRQEAQERAERLYAEEKARLEA
                     EVDFIRKHIAGGQTDIAKGRLRQLTRDLTLIEQIGLVAMAEARRGGQSWLELGARART
                     MTLNEVDERIRAIRPPGDRGPRLTIRLGTFERGARVVLRLKEAVIGYPERPLFTAAQA
                     KLERGGRVALLGPNGSGKSTLLRTILGELYPLDGEVALGDDVTVGYFAQAHDRLVPER
                     RIIDEVWARADMGQTEARGYLAQYLFRGDDVFKRVADLSGGERGRLALALLALEGANF
                     LLLDEPTNHLDIPSQEALQEVLEAFAGTILLVSHDRYLVDRLAREIWSIEAGQLVVYP
                     ASYQEYIALRDGTSAPPEVEPLEDTAPSAPTASVEQPPAAAEEMPAIPRGWSKSARHR
                     DDRRRRQIEESMEDVECWLDQATEALEAARAAADEGEILVLEAECAEAREQLDALQAE
                     WELLA"
BASE COUNT         3249 a         5971 c         6108 g         3687 t
ORIGIN      
        1 tgttgtgtta tactgggtgt cattcgaatc aaacgtcaca gccgggggaa gacgatggcc
       61 aggcaagata aagtcgtcgt gttggcagtt ttgttcgttt ttgccgccct gattggcgcg
      121 gcatgctcga attcacggtc cgagcgcgcg ccggagcagg acggccgcgt ccccttcgat
      181 ttcctgtccc aaccattcaa cgagcaggag gctatcgcct ttatcggcag ccccttgccc
      241 gccggggcga ccgatgtgca tgtcgccggc gagtcggccc tggatacgat agtcgtcgcc
      301 cgcttcgacg cgccccagga cgacgtcacc gcctggctgg ccgagctggg catcaccgag
      361 tcgctggagc cgggctattc acccttctat tcctccggac cgtcgctcag cgaggcggcc
      421 gactggtggg aaccggcgac ggcggattcc accgaggtcg attacagcgg cctctatcaa
      481 caggtggggg caaaatacta cagtgtcgta gtgacgccgc ttgagaatgg cctggtgagg
      541 gtccatcttc tggttcacaa tacctgagca ggggaggtgc gatgaggcga tcggcgaggc
      601 gaacgcggat tggaacatgg ttgatcgggg cggcgctggt cggcatcctg ttaaccggct
      661 gctattctac accggactct ggacggcagg agatgacgac agtgccagag ccaacttatg
      721 tgcaccaact gagcattacg gaaacgacag taggcaggct agacgatggc tggagtgtcg
      781 gggtgcaatg ggtatccaac gagcgctacg tggatgcgga aggcaaagag cggagaggcc
      841 ttgtcgcgcg catcagcgtg gggaacgacg cgttggagca aagtgacacc ctggaggtct
      901 acgagggcat ggttttcccg ataggtcagc agcgctacca ggtggtcaag ctcaagccca
      961 acagatcact cagcgtcgcc cccggcagca gcaacggtta tatcgtgatc gggcagttgg
     1021 cggatgactc cacgggtgcg ccggagatgg gcggcatggg cctgaccgcc ggcacggtcg
     1081 aacggctgtt tgaagacggt tccgcccatg tttatcgtca ggatggcggc gagacgatcg
     1141 atctcttctg tcacgtggtc gaaggcacgt ggtgctactt tgtgccgcag ggccaaatcg
     1201 aaccctacga tggcctctat gaccctctga tcgaggacga ggcgcgccgg atcacctcgg
     1261 atgttgccga acaactcacg aaggagctgg gcgtcgaaac tatcgcccag tcgcgcaaca
     1321 acggcgtcaa actgcgttgc gcccggccgg aggacgccct ttcgcaggtg acatgccggg
     1381 tcgatcgcgg tggtggctgg cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnagatctat caggccatgg gccaactcat cgtcgacgcc atgcctgagg cgtggctcag
     1561 cgccacggcc tgtgccgaga ttgaggagga cgacaatggc ctgacctggg gcaggtacgt
     1621 tccggcggcc gcgccggacc aacaacgtga cttcgatcca gattatcgct gctacctgat
     1681 gtttgacgag ctgcgccgcc gcacccgcaa gcccggccac gcgctgtggg tgaaagcccg
     1741 cttcacgtta cagcccgacg gtcacttcga cctggatttc acctacccgg acgacggttc
     1801 ggagtaagag taaaggaggc acccatgacc ggctcggata cactttgcgc ccaattcacc
     1861 gctcatgtcg gctcttgttt cgggaagatg ttcgaacagc gttcctttga gccggcggat
     1921 tgcctatgtc ttcgcgacgg gcgggaatgc atcgcgctct accgatctcc tgccgcttcc
     1981 ttgctggcgg agctgtcgga tggagcgttt cacatcctgc tcggctccgc ggatgcgccc
     2041 tttcccggcg gtacttacat agaccacgcc ggtcaatccg gttggtatgc gctgttcctg
     2101 ctggtcgagt tcaggagcgg ccaacagatc cacacgaaaa aggtcctgaa gcgcttcctg
     2161 gacggagaac ttgacccata tcgcttcgag gccgacttgt tcgagcgttg ggcggatcgg
     2221 attctgccca tgttcgagac cactcaggag cagagttggc gagccgagtt ccgccgctac
     2281 ttcggctatc cagatagaga gaaccgctga tgatcgaacc ggtcttgaca cctacggagt
     2341 tggcgaacgc ccggatggct ctattcgagc gaggtgttct ggtatgtgcg tcactcgagc
     2401 cggaggagat ggccgcttgt cgccgtgttg gcgattatct gataccccga agtaagcttg
     2461 taacgcatga agggcagcaa ccatgaacac ggatggtttt ctggcattgc ttgaggcatc
     2521 tgcgccatcg tcgtgggaag tgctgatggc tgaatatgaa gggcctttgc cggcagcgtg
     2581 cgacgagcgt tatcacaacg tgtgccaagc ctatcgccag ttgtctccac cagaacgatg
     2641 tgaggcagaa gaacgcatgt cgaccgagca acgtgactgg atgcgcgctg tgcctgaccg
     2701 catggctatg ctcagcgtgc gcctgcaagc cccggcctac ctggaagacg cgttgatcgg
     2761 tgcattcatg aactttcctg ttgctgtcac tgacccgctg gagttccagg ggtatcgggg
     2821 gatcctggcc atttgccggc gatgtgcgca gcttctgggt ttgggacccg aggcgttcga
     2881 gggccagatg atctacatct gccgcgatga cgcgcgccgg gatgtgcgtg agttcttgat
     2941 gggggccgct tcagaccagc agccggcgcg ctggggcttc cgcgaggaaa caggtgatca
     3001 tgggattgtt ttccgttggg ggcaattgcc tatccccgaa gcctggctat gatcaaactg
     3061 gacggattcg gctgtccaaa cttcatcgaa catttgtgga agcaaattga ttgcgccaat
     3121 tttgtaaaga taaagtagcg gggaagtgtt gctgatggct ttaggcatga gcgttttcta
     3181 aatcatcgag ttcggatgca aacgggaaaa ccttatagcg attcaagttg agtaaaaact
     3241 caattctgga cattcctgca agttcggacg ctcgcccaga agaaagcctg ccaatttcaa
     3301 acaacttgac tgccgctaaa ataagaattt cacgcccaaa agattcagcg tccgtctttt
     3361 ctgcgagaag taccttttct ggaatttcga tagtaatttg ttggagagtc atttttactc
     3421 cttttttcca ccgctaattt tactcgcttc aacttcgatt tttattgcaa ctttcgtttt
     3481 gcgtggggcg tgccaacggc tggcgttact tgcgggtggg cgggagtgga caaaactccc
     3541 agagcaggat tctgttcggg tgtagataaa gctccgaaaa tgccgcagaa tcccacccct
     3601 caagtgcacg ctttgttagg cggcttttgc cataaggcta cttggctttt agaggtaact
     3661 tttccaattc tctgtgtagc atttcttcca actctctaat gcttttgtaa atcaaacatc
     3721 tttgcgattt cacatcgaac tttaattcat ttgcgtcttg cacaagaaga actgtttgct
     3781 tttcacaccc ccaagcataa ccgacttcca gatatacatt tgcattagcc tgtgttaaat
     3841 ctgcaagaac taaatctgct tgcctgatac gcaatttcac atactccatt acatcgcccg
     3901 tgaaatgcgt ttgatctact ctttcacata gataacctgc gttatttact gctcccctta
     3961 ttccgtaatg aaaaacatca ctcatttttt catcaaaggg cattgcaaca aaaactaaag
     4021 gctttgtttc tgaagataaa cccgcttctt tcaatcggct agaagaagat gaacttgttt
     4081 cagccaaatt gcctttgttg ataacaataa agccttgcgg aaacaaatct ttaagcgatt
     4141 gacttaattg ctctgcaaga cttttatgta gttctacaat tatgatttct ttgagtcctt
     4201 ttggaaattc tcctttcgaa agagcgtcaa caattcctgc aatttctgat tcaaatgctt
     4261 caacctcatc taacccataa ccgccgccat gaagggtaaa acaaatcgtt tcaacctgtg
     4321 gcgaattctt cgcaagataa gccaatgctc tatgagcaaa ttccctgaca ttctgatacc
     4381 gaaattcaaa taattgctct acgccaacaa atagaacttc cttaacccct atcgtgccaa
     4441 atgaatcaac ttgtagaaat ttcccatatt caggaagtga tgagtctaaa tttgcatgta
     4501 attttgacag ccgatgaaca accttgcggt caacgccaaa taatgcttga gcatgtttca
     4561 gcaccaacac atcggcattt ataaccagtg cgtcattgtt tactaccctg attgaaattt
     4621 gcattgactt gtttctccat gacttctgga taaaaggggc cgcccaacta ctgattcgcg
     4681 ggaatgccgg tcgcccgatc ccgccggctt gctattccgc tttttgtccc tctggcgggg
     4741 gttatgcgga atgctcgccg cataacgctc cacatgggat tttatccggc gatggacttg
     4801 tgctaaaatg cggatggtgt gggcagttga aaactgctca cgctgcttcg tcgctcccat
     4861 cctaacatcg ctcccgtcct ccgtcaaacg ccaggaatga gcgccgaacc aaaggtgaaa
     4921 aggccagtgc catttaagcg aatggtcaga tcaacaggtt ccccacgacc tgtggcggcg
     4981 tcgccggctg gttggagcac aaatggttcg actgagcata gacgggatct tgacttgtgc
     5041 tgacctatta tgacagagcc gtgggcatcg tttgcctgga gaccgatgtg ttcgagtacg
     5101 aggccgatct gtcgaaactg cttggcttgc tgccggcatc tcctttgatc tggcaatgga
     5161 gcagcaccag gcgctaccct gttcatcctc gttatgccgg cctgaaactg ccggctcatc
     5221 gcgactggac gcgccttact tctctgagcc tggacctcct ggcggccgat ctggtcgagc
     5281 aagatctgaa tgacgaccga agacaggcgc ttctgattgg atctgatcga gagttgatcg
     5341 gcacactgtc gaataccgag gaggccagat ctgtattgga ggactttgca gcggaacgac
     5401 ctccggtccg catggcagtg gcacaacttg gcgattcggg gtcaagcttc attttcatgc
     5461 cgcgccatcc ttccaggtcc gtgcaacatt tgcttgaggg gtggggcgtt tcaatcgctt
     5521 ccgcgcgcaa gcgtctaccc tacgggcagc tgcatttggc ccgctttgag gccttgtttg
     5581 gactccggac ggctccatga accagtcatt ccgattgccg gttgccgagc ctgccggacg
     5641 tgacgacgag gagacatgac agaaccacgc tctttttcac agcgtatctt cggccattcc
     5701 ctgcctgccg acctgactga ggccgtcggc cgcaacaccc acgtgccgct gctgggcgag
     5761 ggctggttcg tggccgtctt cgtctggggt ggcgcggtgg ctgcgttcac ctttgtcttc
     5821 ttcggatcgg tgttcttctc cggcatgccc cacgccaatc cggccgccgc gcgactgacg
     5881 ctgtggctct cgctgttcat cgggctaatc ggcggcgtgg ccgggctggt cgtctatcgg
     5941 cggctgcgtc gttaccggtc gccgggcggc acggaggccg atctccatcg cgcggtcgcc
     6001 ctgcaccggc ggctggtggc ggggctgttg ttgctgctgg ggctggccgg gctgtggcat
     6061 gcctggctgt tttcgcccgt gacgcgcata ctgggcagcc tctctttcat catggcgctg
     6121 gcgctgggct tcgtggcctt gccgtccttg ctggccctgg ccgccggcca atcccggcgt
     6181 ttcggccgcg ccgcccaaac gctggatcgc ctcctgctgc tgggaatcgg cctgaccggg
     6241 ctgttggtcg ccgccccgcc gatagcgcgg gccgaacccg gcgcgttttt tctgctcctg
     6301 acccaggtcc tgggcctgat cgcgctgccg tgggtggggt cgatgctggc cgctgcccga
     6361 atccacagcc ggatgaaatc ctgattacag gcgctattta ttgcgcaacg cctgccattc
     6421 gcgcacgatc ttcttctctt cctcgctcag cttcgccggc agttcggcgg cgacggtcac
     6481 gcagaggtcg ccgcgctcgt cgggcttgcg cggcttgggc atgcccagct tcgccaggcg
     6541 gaacaccttg ccgttgcgcg ggccttcggg gatggtcagg ttgaccgtat tcaatgcaac
     6601 cccaaccggc ccatctgcgt aataatctcc agtatgatgc cgggtgcagc gcgtattcgc
     6661 gccttgtttg aacgcgaaca aatcgagggg ttggccaccg accatgccac ggctcgcggt
     6721 gccgacgacc tgctgcgcgg cgagggctgg gtggcggccg ggcgggtcac gcttgagccg
     6781 tcgggcgatg atctgctgtg ggccgaatac cccgtgtcgc gccgtgcacc cgttctaacc
     6841 accgtcagct tgcccggcct gtgggcgacc tgctcctgcg ccgccacacg cttcccctgc
     6901 cgccacatca tcgcgctcct gttaagcgat ctgaatgaac cgcttccgac cgacgccgat
     6961 gccccggact ggctgcctcc ggccgcgcat ggctggaatt tgactcctgc cgtcggcgaa
     7021 acccaatcgg ccgagcggca ggcggccgtc gtcgccggga tggccgactt gcgcctgtgg
     7081 ctgagcgatc tggcgcgcga agggttggcc gacctgcccc gccgcggccg cgccaaattc
     7141 ctcggtgcgg ccaaccggct ggaggacgcc tacgccgttg aagccgcccg cgccttgcgc
     7201 gagacggccg ccatccccgg cagcggcatg gactggccgg agcgattgct gcccgacctc
     7261 ggccggctgg cgctgttgtg cgagtcgttc cggcggctgg acgcgctgtc ccccggcgaa
     7321 cgtggcgaca cgttggccgc cgccggtttt ctgtccccgg ccacgggcga gcgcgtgacc
     7381 gacacatggc gcatcctcgg ccaggtcgtc gaggtcgaga accgcgtcac ccacctgcga
     7441 tcatggctgc gcggccgggc cacggggcgc tgggcgcggc tcgatcaggc catccccgcc
     7501 gggcagctgc agggagtttg cctgcccacg ggcgcgctgg tcggcggcgc gctggccttt
     7561 tcgcccagcg cgtacccgct gatggcgcgg ccggtcggcc cgctcgcgcc ggtgcatggc
     7621 gaaaccggtg atcccgccgc ccatgacgac atcgacgccg tcatgtcggg ctggtcggcc
     7681 gccctggcgt tgaacccctg gctgcgccgc ttcccgatgg ccatccgcga cgtgtgggtg
     7741 gagccaccct tgacggccgg cggccgctgg cgactgcgcg accgggcggg gcatgttctg
     7801 cctctgccgc cccaattcgg ccacggctgg agcctgacgg cgctgtcggc cgggcggccg
     7861 atgacccttt tcggcgagtg ggacggggcg ctgtttgcgc ccgcgagcgt cctggccggc
     7921 acctggcggc cgctgagcgc ctggaaggtc gtcgtatgag cctgccgccc gcgctcgtca
     7981 ccgccgcgct ggtcggcaca ggccgctccg gcttgcccgc cgcggccgac ctcccgccct
     8041 ttctcgccgc cttgcgcgcc gaaatggccg cccgcccgcc ggaggagacg ctcctgctgc
     8101 tggccggcgc gggggcgttc cacgaggcgg ccgggcggct gccggagcgc gtccccgccg
     8161 ccgagtggca tctgcccgcc tatcgcgccg agggcgatct gcggccgtgc gcgcccgcgg
     8221 ctgagcgtct gctggagcgc atgctcgccg accgggatgt ggaattcttg cccgagctgt
     8281 tggccttgct ggagaaagcc ggggcgcgcg cgcccgaccg gctcctgccg gaagtgctgg
     8341 ctcgcggcgt gaaaatccct cgcctgcggc cgtctctgct gccggtcatc ggcgagcgcg
     8401 gccgctggct ggccgcgctg aatccggcct ggcgctatgc agccgtcgat ttggccgatt
     8461 ttgagcagtt gcgcgccgtc tggaatgacg atcccggtgg gcggccttat ctggcgacgg
     8521 ccgtccgtcg ccgcgatccg gccgccgccc ggcggctgat cgaatccacg tggcgggccg
     8581 aatcggagat catccgtcgc gatctgctgc ccgtgctgga gaccggcctg tccatggcgg
     8641 atgagccatt cctggagcgg gcgctcgatg atcgtgacgc ccacgtccgg cgtcgggcgg
     8701 ctgatctcct ggcccatctg cccgaatcgc gcctcgtcgc ccgcatgacg gccgccgccg
     8761 gcgacatcct gagccttcat gacgatgtgc tgaaaccttc cgcccagcgg cccgtcactg
     8821 acgcaatggt gcgcgacggc gcgaatcgcg acaaggaacc cgacggggtg cgaacggcga
     8881 ccgagtggtc gaccctgctt ctccgcaccg tcggggcgat tcccctgacc cattgggagg
     8941 cgcggtttgg cctttcgccg gaggcgctcg tcgccgcatc cctggccggc aagtggccgc
     9001 gcacgctgct gaacgccttc gcgacggccg cgctgcgaca gcgggacatg cgctggatcg
     9061 atccgctgct ggagggggat cagtacacgg agcgcaccgg catgctggtg gcgacgctga
     9121 cgccggaggc gtgctacgcc cgattggcga ccctgatcga cgccgggaag gacgagtcgg
     9181 tgtcggtgtt tctgcgccgc tggcccggcg tgtgggacga gagcagcggc cgcgcgctgc
     9241 tggcgctgtt cgcccgctat tgcgccgatc ccgtcgacgc acggctgaac ctgaccttgc
     9301 gcttcctgtc gcgccagttt gcccagcgct gcccgccgtc gctggcgcag gaagcggccg
     9361 atctcctgca aggccgcgcg gtcaaccggg cctgggaggc gagcctgaac tatctgcttc
     9421 gcacgctggc gctgcggcgc gagctgtggg gggcgaccat tggcgagcgt tcgccggagg
     9481 agaatgattg aatgaccgta tcgactatcc agcgcgagct ggctgaacag gcctatggcg
     9541 acgagctggc cgcattgatg caggccgacc ggcggccgaa accgcccaac tggcgcatgt
     9601 ccccctgggc tgtggccacc tatatcctgg gcggcacgct cgacgacgga accgtcatcg
     9661 cgcccaaata catcggcagc tcgcggctgg tcgagatcgc catcgccacg ctgaccaccg
     9721 atcgcgcctt gctgctcatc ggtgtgcccg gcaccggcaa gacctggctc tccgagcatc
     9781 tcgcggcggc catctccggc gactcgactc tgctggtgca gggcacggcc gggacgagcg
     9841 aggaagtcat ccgctacggc tggaattacg ccagcctgat cgcccgcgga ccgtcggccg
     9901 aggcgctggt gcccagcccg atcctgaacg ccatgcgcgg cggccgggtg gcgcgcgtcg
     9961 aggagctgac ccgcatcccg tccgacgtgc aggacgcact gctcaccatc ctgtccgaga
    10021 agctgctgcc cgtgcccgaa ctgggtgaag cggtgcaggc ggtcaagggg ttcaacgtca
    10081 tcgccacggc taacgaccgc gaccggggca tcaacgagct gtcgagcgcc ctgaagcggc
    10141 gcttcaacac cgtcatcctg ccgctgccgg caacggccga cgaggaagtg accatcgtca
    10201 ccagccgcgt ggccgagctg agccgcgcgc tggagttacc cgccgaacag cccgcggccg
    10261 agcaaatccg tcgcgttgtc accatcttcc gcgaattgcg cagcgggctg accgaggacg
    10321 gccgcgccaa gatcaagtca cccagcggca cgctgagcgc cggggaggcc atctcggtgg
    10381 tcaacagcgg gctggcgctg gccgcccatt tcggcgacgg ccgcctcgcc gccgacgatc
    10441 tggcggcaag cctgaccggc gcggtggtga aagacccggt acaggacgct ctggtctggc
    10501 aggagtatct cgaaacggtc atgcgcgagc gcgaagggtg gggcgatctc tacagcgctt
    10561 gccgggaact ggcccgatga gcgaggcgga gttgcgcgtc tttggcgtgc gccatcacgg
    10621 gccgggcacg gcgcgggcct tgctcgcggg gctggccgcc tatgagccgg attgtgtcct
    10681 cgtcgagggc gcgcccgacg ccgatgcggt catctcctgg ctggcccatc cggcgctggt
    10741 gatgccggtg gccctgctcg tctaccggcc cgatgaaccg cagcgggcga ccttctaccc
    10801 cttcgccacc ttctcgcccg aatatcgcgc cctgcgtttt gcgctggagc gcgatatcgc
    10861 cgtgggcttc atggacttgc cccgccgcca tgcgctggcc ttgccctatg gcccgacgat
    10921 gccgcccgcc gccccgtttc gcgccattgc ggcccgtacc ggctacggca gttacgaagc
    10981 ctggtggaac gcggccatcg agcagaccgc gggcggcccc gaaatctttc ccgcgattct
    11041 ggagttggcg gccgggatga ggtcggccgt tgaaccgtca tctccgccgg aggacgagtc
    11101 gccggaggac aggatggccc gccaacggga ggcggccatg cggcaacgca tccgcgaagc
    11161 ggccgcaagc ggccatcgtc gcatcgccgc cgtgtgcggg gcgtggcatg gcccggcgct
    11221 gctcaatgca ctggaaacgg acggcgcggc cgacggggca ttgttggccg atttaccgtc
    11281 ggtcgaggtg gaatcgacct gggtgccgtg gacctacggc cgcctggcgc aaacgagcgg
    11341 ctacggcgcg ggcatcgctt cgcccggctg gtacgaccat ctgtgggccg ccggcgaggc
    11401 cggactgccg gccgcagaga tgagcgccct atggctgtcg cgcgtggccg aactgttgcg
    11461 ccatgaaggg atggacacct cgcccggcca cgtcatcgag gcggttcgcc tggccgaggc
    11521 gctggctgcc ctgcgcgggc ggccgttccc caccttgccc gaacttgaag aggcgacgct
    11581 cagcgtcatg tgcgacggcg gccaggagcc gctggagctg attcgccgcc ggctcatcgt
    11641 cggcgagcgc atgggcatcg tgccgcccga cgtgcccgtc gtcccgctgc agcgcgattt
    11701 gaacgcgcag caggcgcgcg tccggctgtg gcccgaaccg gagaagagcg cgctgacact
    11761 cgatctgcgc caggccaatg atctggaccg cagccgcctg ttgcatcgcc tgtggctcct
    11821 ggagatcacc tggggcactc ccgtcaagac ccggggggcg caggccggaa cctacgccga
    11881 ggtgtggcaa ctgcaatggc tgccggatct gtcggctaaa gtgatcgagg ccgctatgtg
    11941 gggcaacacc gtccgcgacg ccgccgccgc ccgggtgaac gacaatgttg cccgccacac
    12001 cgatctgccg aagctggtcg ctctgatcga tcaggtcatc tcggccgatc tgccggaagc
    12061 gttgccggcg gttctggcgc gcatcgagga actcgcggcg ctcagccggg acgtgggcgg
    12121 cctgctggcc gcgctgccgc cgctggccga taccttgcgc tatggcggcc tgcgccagac
    12181 ggccgagcat ctgcccattc tccaccacgt gttcgaccgc ttgctgacgc gggcttgcct
    12241 gggcttgccc ggcgcctgtc gctcgctgga cgagaacgcg gcgaacgaga tgatcgagcg
    12301 gctctcggca gcggcctcgg ccgtccggct ggtgggcaac gagcaggccg ccgaacgctg
    12361 gcaggccgcg ctggccaggc tggccgacct gccggacatc caccccacgg ttgccggtcg
    12421 ggcgacccgg ctgctgttcg atgaggccgc gattcggccg cgcggcgtgg agttcatgat
    12481 ggcccgcgtg gaacgggcgc tgacgccgcg aggctcggcc ggcgtgaccc gcttcgccgc
    12541 cgattggctc gacggcttcc tgcgcgacag cggcctgctg ctcgtccacg acaaggcgtt
    12601 gtggggcgca atagaccact ggctcaccgg catgggcgag gcgcagttca cgggcatcct
    12661 gccgctgctg cggcgcacgt tctcgtccta ccccgaagcg attcgcgagc agttgcaatc
    12721 ccgcgcgcag ggcggccggc agcggcaagc gagcggtgaa gttccacagg cccgattcga
    12781 ccccgaacgc gcggcggccg tcgtgccggt gttgcggcgg atgctgggct tgccccaacc
    12841 gccgaaggag acagcgctat gagcgaatcg gccgagcgcg ggcggcgctg gcagctggtg
    12901 ctgggggcga cggagcagcc cgacaacaac gagggcggcg atgattcggc cgaaggcggc
    12961 ggggcgcgtc gtcaacagca gcagctctcc ccggccgaca gggcgctgga tgaggctttg
    13021 ggcgcgctct acggcgacag ccgcggcggc gatctcagcc ggtcgatgcc cgacgtggcc
    13081 cgctggctgg gcgatattcg cgcctatttc cctgaggatg tcgtcgatgt gatgcagcgt
    13141 gacgcgctga agcggctgga tccgcgccgc ctcgtcgagg agccggagct gctggccggg
    13201 attgagccgg acgccgcgct ggcggcaaag ctcctctctc tgcgcaaggt gatgccgccg
    13261 cagacgcagg agacggcccg ccaggtggtg gcgcgggtgg tggatgatct cgtccggcgc
    13321 atcgaatacc cgctgcggca ggcggtcagc ggccgtctga accgggcggc ccacacccat
    13381 cggccgcgca aggctcagga gatcgactgg gggcgaacca tccatgccaa cctgaagcat
    13441 taccggccgg agctgggcac ggtgatcccg gagaagctgg tcgggttcgg ccgccgccgg
    13501 gccacgttga aggacgtcat cctgctggtg gacaccagcg gttcgatggc gacctcggtc
    13561 gtctattccg gcatcatcgc cgccgtgctg gcctcgctgc cgtcgatcaa cacgcggctg
    13621 gtcatgttcg acacggccgt cgtcgatctc accgaccggc tggacgatcc ggtcgagatg
    13681 ctgttcggct tgcgattggg cggggggacg aatatcgatc aggcgctgag ctacgcccgc
    13741 ggagccatca ctcggccgcg cgacaccatc gtggtgctca tttccgacct gtttgagggc
    13801 ggcaacaagg agcggatggt gcggcaggtg cagcacatgc tggccgacgg cgtgcagttc
    13861 atcaccctgt tggcgctcaa cgaccagggc gcggcccgct acaacgccgc cgtggcccag
    13921 gagctggccg atctgggcgt gccggccttc gcctgcacgc cggagcagtt tgctgatttg
    13981 atggctcggg cgcttaacga acagtggcgc taaagcaatt gttcaagaac aacgttgcta
    14041 atcgtttggc atcaacgaac gattcatctt tcatgctgaa agagagacaa attatgctac
    14101 aatttgtcca tatccgaatg tagttatggg taatgggcaa gtttaaatca tgccgactgt
    14161 actgcgcgaa ggaaaatacc ggttcacttt cttcagtaat gaaaaccgag agccttcgca
    14221 tatacatgtc aaagctggcg cagatcaggc aaagttttgg ttagacccga ttgagcttgc
    14281 atcaaattat ggcttcaaag ctcacgagtt gaacgaaata gagcggataa tctatcgaca
    14341 tcaacctgat ctgctggagg attggtatga atactttagc tgagacgcaa acggcaacga
    14401 aacatcagtt ccgccgagcc cacgtgcccc agtcggcgct ggcgaaatcc gttgagttct
    14461 ccgacgactt gatgcacgtg ctgctaacgg atggccgaat catcagcgtg cccgtcatct
    14521 ggttcccgct gctgcacgaa gcgacggccg aacagcgggt gaactatgaa atcggcggcg
    14581 gcggggtaag tctgcattgg cccgacatcg atgaggatct atcggtagcg ggcctgctgg
    14641 ccggggccga ttggcaagcg tcatgaacgt ttcacgccgc ttgcaatccg tcaccgctac
    14701 ttgccacccc taatttcccg ccattcgcgc acgatcttct tctcttcctc gctcagcttc
    14761 gcgggcaatt ccaccgctac cgtcacatac agatcgccgc gctcgtcggg cttgcgcagt
    14821 ttgggcatgc ccagcttcgc cagccggaac accttgccgt tgcgcgtgcc ttcggggatg
    14881 gtcaggttga ccgtcttgtc gatggcgggc acgctgatct tgccgcccag caggacggtg
    14941 aagatatcca ccggttgggt catgcgcagg tcgtcgccgt cgcgctcaaa gatggcgttg
    15001 ggcagcacct cgactttcag gtacaggtcg ccggcgcggc cgtcggcgcc gcccgcctgg
    15061 ccgctgagtc gcactttcga gccggtgcgg acgccgcgcg ggattttggc atccacgcgc
    15121 ttgccgcctt cccattgcag ggtgcgcgtc gtgccgctga acgcctcttc cagcgtcacc
    15181 tggatgggat gctcctggtc gagccgctgg ggggccggcc gcgcgcccat gccgccgaac
    15241 gggtcatcgg ccgacgaaaa ggagccgccg aagccaccgc gcgaccgccc ggccgagcga
    15301 gcgcccatcc cgccgaacaa cgtctcgaag aagtcggaga agccgctgcc gccatggacg
    15361 ccgccgccga acatctgctc aaactcttcg ggcgagacgg tgcgatactg gccgccgccc
    15421 gcacccggcc ggccgcccca cgccccccaa ttgaagtcct gggcctggcc gcccgcgccg
    15481 gagaactgct gccattccgc gccgaactgg tcgtacttgg cccgcttctc ctcgtcggac
    15541 agcacctcgt aggcctcgtt gatgtccttg aacttctctt cggccgcctt gtccccctga
    15601 ttcacgtcgg ggtggtgctt gcgcgccagt ttgcgaaatg cttttttgat ttcatcagcg
    15661 ccggcgtcct tcttgacgcc cagcgtcttg taatagtcct tataatccat attgattgat
    15721 tatgccatcg acaggtcaga aatgggtaag agttgggagc aattttcaag attcatgcaa
    15781 agaggcagcc aattgggccg cctctttgtg tcattattcg gttatcgggc gaacggatca
    15841 ggcgcgatcg cgggtggcct ggccttcggc ccagccgcgg ctcttcggct cggcctcggg
    15901 gggcagcttg gcctggccct cagcccaacc ctcgctcttc ggctcggcct ccgggggcag
    15961 cttggcctgg ccctcggccc aaccttcgct cttcggctcg gcgctgaccg gcaggtcggc
    16021 ctggccctcg ccaaagccgt gactgcggcc gttgccgttg ctgcccctgg cctggccctc
    16081 aagcgtcttg cggccgtggg tcttgttgac ggcgatgcga tgtggccgaa cggcctcggc
    16141 cttcggcagc gtcaggatca gctcgccatt gacacacgac gcctcgatct tgttggcctc
    16201 aatcggcatg gggaaggtga tgctgcgggt gaagctgccg aagcggcgct cgcgcatcat
    16261 gtagcggtct tcgtccttct cgtcatcctc gcggcgcgtt tcgccgtgga tggtcaggac
    16321 gttatcggtc agggtaatgt cgagcgcgtc ggggtcgatg ccgggaatgg aggcgtggat
    16381 gacataggcg gcgtccttct ccatcacatc cagcggcagg ttccaggatc ggccgccgtt
    16441 ccagtcgacc atattgcggc cgacgtcgcc gagcatgcga tccatcatct ccaccgggtc
    16501 gcgatctagc caaatcggat aagggttgtt ccaacgaatc aagcgtgcca tggtatgttc
    16561 ctccattagc gtcgaaccgg gcggccgacc aacggccgat ccggcgccgg gctggttgac
    16621 gtgatctccc ggcgaaatag attgattttg attttcaact gagtgtgatc ggtagaagcg
    16681 gggcggtgcg ggggcgtcaa tcgtgtggcg tgtccgatgt cctgttgcca atcaccttga
    16741 ttactaccat aggcttctat cgtaagaatt gagttagatg tatataaaat ataaatgaga
    16801 aacggttaat tgcattagaa accgagtttc ttccgtagaa actcggtttc taatggggtt
    16861 tctcggaaga aacccggttt ctacccgcac cctcgccaca accccgccaa agcgatagaa
    16921 tgaacggctg ttatgtctgt cctgaccgcc gccgaattga gccacgccta tggcgcggcc
    16981 gagctgttcc atgacctcga cctgaccgtc gaggcgcggg atcgcgtcgg cctggtcggg
    17041 ccgaacggcg tgggcaagac aacgctgctg ctggcgctgg ccgggctgct ggagccgctc
    17101 agcggccgcg tcgatcaggc cgccggtctg accattggct atctgcgcca ggaagccgtc
    17161 ctcaccttcg ccgggcgcga caactccgtc tatcaggaga tgctgaccgc cttcgccgac
    17221 ctgcgccgcc gcgaggccga tctgcaggcg atggaagccg ccatggccga cgattactcc
    17281 gacgaactgc tcgcgaccta tggcgcattg caggaggcgt tcgagcgcga gggaggctac
    17341 cagtatcagg tcgagatcaa gcgcgtgctg ctgggcctcg gcttcgggca ggcggaatgg
    17401 gacaccccgc tgctgcacct gagcggtggc cagaagacgc gcgtgctgct cggccgcctg
    17461 ctgctggaga aaccggccct gctcatcctc gacgagccga ccaaccatct cgacatggcc
    17521 gccatcgaat ggctggaggg gacgctgcgc cgctgggatg gggcgctcat catcgttagt
    17581 cacgatcgct attttctcga tcgggtcgtc aaccggatgt gggagttatc acctgacggc
    17641 aataactcgg ccgagctgcg cgcctggcgc ggcaactaca gcgccttcgt cgcccagcgg
    17701 caggaggccc aggagcgcgc cgagcggctc tatgccgagg aaaaggcgcg gctggaggcc
    17761 gaggtcgatt tcatccgcaa gcacatcgcc ggcggccaga ccgacatcgc caagggccgc
    17821 ctgcgccaat tgacccgcga cctgaccctg atcgagcaga tcgggctggt ggccatggcc
    17881 gaggcgcggc gcggcgggca aagctggctt gaattgggcg cgcgcgcccg aacgatgacc
    17941 ctcaacgagg tcgatgagcg catccgggcc atccgcccgc caggcgaccg cggcccgcgc
    18001 ctcactatcc ggctggggac gttcgaacgt ggggcgcggg tcgtgctgcg gctgaaggag
    18061 gcggttatcg gctatcccga gcggccgctc ttcaccgctg cgcaagccaa actggagcgg
    18121 ggcggccgcg tggccctgct cggccccaac ggcagcggca aaagcaccct gctgcgcacc
    18181 atcctgggcg agctgtaccc gctggacggc gaggtggcgc tgggcgacga cgtgactgtc
    18241 ggctacttcg cccaggccca cgaccgcctc gtgccggagc ggcggatcat cgacgaggtg
    18301 tgggcgcggg ccgacatggg tcagaccgag gcgcgaggct atctggcgca gtatctcttt
    18361 cgcggtgacg atgtgttcaa gcgtgtggcc gacctgagcg gcggcgagcg cggccggctg
    18421 gcgctggcgc tgctggcgct ggagggggcc aacttcctgc tgctcgacga gccgaccaac
    18481 caccttgaca tcccctcgca ggaagcgctg caggaggtgc tggaagcgtt cgcggggacg
    18541 attctgctgg tctcccacga tcgctatctg gtcgaccggc tggcgcggga aatctggtcc
    18601 atcgaggccg ggcaactggt ggtttatccg gcgagctacc aggagtatat cgccctgcgc
    18661 gacggcacga gcgcgccgcc ggaagtcgag ccgctggagg ataccgcgcc gtccgccccg
    18721 accgcgagcg ttgaacagcc gcccgccgcg gccgaagaaa tgccggcaat cccgcgcggc
    18781 tggagcaaga gcgcccgcca tcgcgacgac cgccgccgtc gccagatcga ggagtcgatg
    18841 gaggacgtcg agtgctggct cgatcaggcc acggaagcgc tggaggcggc ccgcgccgcc
    18901 gctgacgagg gcgaaattct ggtgctggaa gcggaatgcg ccgaagcgcg cgagcaactg
    18961 gatgcgcttc aggcggagtg ggaattgttg gcctgattat aatttaatac agacctgaca
    19021 ggtgggcgat ccaaccattt ggccgatcga agtctcaatc gcccacctgt caggtcttga
    19081 agctgttagt gagagtgaac gattatgaat gaaac
//
LOCUS       DAOMQG010000024        18762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1284_length_18762_cov_11.608222, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000024 DAOMQG010000000
VERSION     DAOMQG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 18762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18762
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1284_length_18762_cov_11.608222"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1355)
                     /locus_tag="PLC06_02820"
     CDS             complement(66..1355)
                     /locus_tag="PLC06_02820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRO89408.1"
                     /translation="MADYIVMPKLGFDMREGVLNRWLKGVGDPVSKGEIVAEIESDKA
                     TLELESHVAGTLLHLLHEAGDVVPVGANMAIVGQQGEDISGLAGGNGSQAAAVAAAPV
                     AAEEVTKQAPSEVSVSAPSTTEVATNDAKPDESYPGGVKATPVARRLAGEKQINLLQV
                     KGSGPDGRVTKADVEAFVAAPQPKPVATPVQVPTASPVEIGPESEAIALTRLRQAIGR
                     RMTESKTTVPHFYVTTEIDMEAALDLRKQINATLPDDAKVSVNDIIVKAAALAVRDFP
                     NLNAELSGDKIVRHKRINIGSAVAVEGGLTTIVQKDTDTSTLSKVAKDNREMIARARD
                     GKVRPDDVEGGTFTVSNLGVYEVDHFIAIINPPEAAILAIGSARKVPVVKDGQLAIGT
                     RMKATISADHRVTDGAEAAQYMQRFKQLLENPLRLLV"
     gene            complement(1355..2326)
                     /locus_tag="PLC06_02825"
     CDS             complement(1355..2326)
                     /locus_tag="PLC06_02825"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRO89409.1"
                     /translation="MAMITMRQAITDALREEMQRDERVFIMGEEVGIWGGTYAVTRGF
                     YDEFGARRVRDTPISEMAIGGVANGAAMNGLRPVAEFMTINFAFLALDQMINHAAKVH
                     YMFGGQIKCPVVYRAPGGGGRQLGATHSHTPDVIFAHFPGFTVVSPSTPYDAKGLLKA
                     AIRSDDPVLFIEHHTLYQIRGEVPEGDYVVPIGVSDVKREGSDVTIVAYAQGLQVALE
                     AANQLAKEGIEAEVIDLRSLRPLDMGPVLESFRKTFRAVIVEEGHRTYGVGAEIAARL
                     QEEAFDYMDAPIKRVAQFEVPLPYSRELEQSALINKDRVIAAVKEIL"
     gene            complement(2354..3328)
                     /gene="pdhA"
                     /locus_tag="PLC06_02830"
     CDS             complement(2354..3328)
                     /gene="pdhA"
                     /locus_tag="PLC06_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRO89410.1"
                     /translation="MEKATLLEWYREMVLVRRFEQKCAELYQMGKIGGFLHLYIGQEA
                     VAVGSIGARRKQDHVITAYRDHAHALVVGSDPKALMAELLGKATGVSKGKGGSMHMAD
                     VAHNYWGGYGIVGGHVPLGTGIALAEQYKGTDAVVLCYMGDGSTNIGYFHEALNMAGV
                     WELPIIYIVENNQYGMGTSVDRASAVPDMSTKALAYGMEPKKVDGMNVIEVYDATKAA
                     IDAIHQGKGPQFLEIITYRFEGHSMGDPLRYRTKSEVEKWREDDPIGVLERIIYEKKA
                     ATKGKLEKIDAEVDEVIDEAVEFAEKSPFPDPEALYEDVYVDRKVERQ"
     gene            complement(3466..4440)
                     /gene="thrB"
                     /locus_tag="PLC06_02835"
     CDS             complement(3466..4440)
                     /gene="thrB"
                     /locus_tag="PLC06_02835"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRO89411.1"
                     /translation="MSSSPALFLRPITVTVPATSANLGPGFDCLGLALELRNELTLLA
                     TGPVLEYGAGETHYTIQVGGVDEEKVPANKQNLAIEATELLFARIGYRPTRLTMRMVN
                     GIPVGSGLGSSSSAIIAGLLGANAIFDHAIPRNELLRMAVDIEGHPDNVAPALLGGLV
                     LGVLPDTESGPDELIVRQWDPPNLSAVVVLPDFKLLTSEARAVLPPTVSRGDAIFNTS
                     RLGLLIHALTAPEFSLLKPAMSDRLHQHFRLRLIPGAVDAYHAAYEAGALGVALSGAG
                     PSLLAFCDNHQVVISEAMQSAFTAAGLASRTWGLKPTAQGACVFRDGE"
     gene            complement(4469..5485)
                     /locus_tag="PLC06_02840"
     CDS             complement(4469..5485)
                     /locus_tag="PLC06_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRO89412.1"
                     /translation="MLTNQLSTNGDAPNPTQFTSVNYGGQAVIEGVMMRGSRALSVAV
                     RDPQGNIVVHTEPLDPRIYGGRLAKIPFLRGLTLLWDALGLGIKSLLFAAEVALQEEA
                     GKDADGKPEKVFEGPVQWTLVVFSLTLSILLFFVLPTFLAHLIERLAGLENSPVIVNF
                     IEGAIRLILLIGYIWAIGLMSDVKRLYGYHGAEHKTINAYEAGAELTPASVARFPLEH
                     PRCGTAFLLSVVVISILVYSLLPPLDLLPRILSRLILLPVIAGIAYEFLKFTATHQGN
                     ALIRLITKPNLALQHLTTREPDIDMLAVAIAAFENVLDYEHNQAAVQLGDTILSPVSA
                     PTEA"
     gene            complement(5555..6625)
                     /locus_tag="PLC06_02845"
     CDS             complement(5555..6625)
                     /locus_tag="PLC06_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89413.1"
                     /translation="MTVTEVRAFPVSSTVVMRSTLFALANMGATLQTYNEESGVIVAT
                     VSKWMGLQKQEVVARVRTFEGTSQLELDAPDFDKARELLQLIASYVRDGGRVQANATM
                     QWVDLQRMQASKAKRQEMSNKARRLLTGSATTSLPAVVEEGPSALVATDDPAQPVGPS
                     MDAPIAIPDNPGVLVKNRQNMLLELKVDPEVFTDRSGFVTMCNVCYAPTLRGSAFCPN
                     CGRPLTLEAVQPEMREKAGKSANSSLTYGLIGLALNAVPLLVLVLPALLSQGEASLLD
                     SIRATLTPLTIALAVVLGILPAMLLGFAALRQGQRASWYLNLPAVLEQSGRGKAGFGG
                     ALGWLSIYLGIGWILLIIIALL"
     gene            6785..7138
                     /locus_tag="PLC06_02850"
     CDS             6785..7138
                     /locus_tag="PLC06_02850"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRO89414.1"
                     /translation="MSFFKRDRTQTDDEFIDVAGTESVREGQMLTVEVQKRKIILTRH
                     AERVYAIDTICPHAAADLSKGSLRSWQICCHEHDYCFDIRTGRIVWPEDEVYRLRRFE
                     VKEENGRVLVRLSTV"
     gene            complement(7210..8562)
                     /locus_tag="PLC06_02855"
     CDS             complement(7210..8562)
                     /locus_tag="PLC06_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRO89415.1"
                     /translation="MHKNILALFLVAGFLLAGCGAKESLPAPTQEPTTVAQPTTLPSP
                     VVTVETNVTSPAEAPKPTEVATEPATTPTKTATEEAPEPTDIPARPVSAISLEPVITE
                     GLEMVTGITHAFDERLFVLEQVGRIRIIEDGQLLEQPFLDITDRVGSSSSEQGLLGLA
                     FHPDYATNGAVMQGQFFVNYTDYSGNTHISRFRVMMDDPYRADPRSEVEYLAQKQPYP
                     NHNGGSLAFGPDGYLYAGLGDGGSANDPLLAGQDLSTFLGKVLRLDVDSTANAYAIPA
                     DNPFLNTPDAQPEIWALGLRNPWRFSFDRETGDFYIADVGQNIWEEVNFQPATSPGGE
                     NYGWNIMEGNHCFEADTCDQTGLTLPIFDYDHSQGCSITGGYIYRGQTYPELWGNYFV
                     SDFCSGVIWRLFPQGDGWLADAILDSDLIISTFGEDVNGELYVANYANGAIYHITPSE
                     "
     gene            complement(8669..9793)
                     /locus_tag="PLC06_02860"
     CDS             complement(8669..9793)
                     /locus_tag="PLC06_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRO89416.1"
                     /translation="MSETKKEDLMTTSSSFVDGTKGELIYRGYDIRDLGPNATFEEVV
                     YLLWNDKLPNENELNRFKAALESKRALPEPLLIIMRDFPWEAHPMAVLRTAVSAMGLL
                     DRNADNITTDAAREKALLLTAILPTLVAAWERLRNGRDPIPPRQGLGQAANFLYMLDG
                     KEPNPDAVAALDSYLVLLADHGFNASTFSARVTTSTGTDVYSAITSAIGTLKGPAHGG
                     ATQRAMEQFIDAAQRGDVEQWFHDARESGKRIMGIGHRIYKVEDPRARILRPLAQKLA
                     ESSGTGKWFDIAREIEQLARKDDYFIERDLYANVDYYSAVVLYTIGLPVDTFTPIFAM
                     SRIAGWCSQVLEQLADNRLIRPKEGYVGERGRQYVALADR"
     gene            complement(9829..10857)
                     /gene="rfbB"
                     /locus_tag="PLC06_02865"
     CDS             complement(9829..10857)
                     /gene="rfbB"
                     /locus_tag="PLC06_02865"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRO89417.1"
                     /translation="MKNILVTGGAGFIGSNFAHYMLSRHNDYHIVVYDKLTYAGNLDN
                     LLDASSNPRYSFVRGDICDQAAVERAVKEHDIDTIVNFAAETHVDRSIMDPDAFIQTS
                     VYGTYTLLEVAKSLSLERYHQISTDEVYGHIPFGKSSKETDKVDPRSPYAASKASADL
                     LVNAYFITYDLPVTITRGSNNIGPYQYPEKVVPLFATNAIDSLPLPVYGDGRQMREYQ
                     HVVDHCEAIDLVLHRGTIGETYNIGTGVEVENLAMVEVLLDTLGRPRSLIRHVADRPG
                     HDRRYSLDISKITALGWEPDHTPEEAIIETALWYKNNEWWWRKIKSGEFRQYYEAQYG
                     ERLRATAQ"
     gene            complement(11059..11910)
                     /gene="nudC"
                     /locus_tag="PLC06_02870"
     CDS             complement(11059..11910)
                     /gene="nudC"
                     /locus_tag="PLC06_02870"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRO89418.1"
                     /translation="MGLSFTANITPPATDEPGLWFIFKGFKLLVVRHETDVAIPQLLS
                     PSDIGLPVERPIYLGTLNSSTGSIHCYCGEVSESTEAPDGYAFENLRPLYSLLDEDSF
                     WLAGRAVQIVDWDRTSRYCGRCGHETVTQTVDRSKICPQCRLTDYPRLSPAMIVRVTR
                     KTDQGAEILLARAQRFPTAMFSVLAGFVEPGETLEECVAREICEEVDIKVRNIRYFGS
                     QPWPFPHSLMIAFTAEYESGEIRVDPIEIAEAAWFTHQSLPVVPSPPSIAHELIQDFI
                     ANSTRSE"
     gene            complement(11921..12649)
                     /locus_tag="PLC06_02875"
     CDS             complement(11921..12649)
                     /locus_tag="PLC06_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89419.1"
                     /translation="MGFSELNNDPAAFRGKRIQVSGVFTPVEAPDCLDHTGPTIRWSL
                     VAEDLQLNAIGFENLVRLLDPETEMTLSGVWSVYQGPVGCGKEPPDGTIWYLAVERIV
                     EPNPLFGAQAPALTVVAGEALPTLSPLETPSSPTPTLTLEATSELSPTMTLDAATTFP
                     MPPTETQDPTLSPVTPLFTPGSTPGQIGTLPPGATPEGTPTFGPSPTPGLEGTPAPGL
                     PTNTPSGTGYPIDPSPSPTTEGYP"
     gene            complement(12892..14799)
                     /locus_tag="PLC06_02880"
     CDS             complement(12892..14799)
                     /locus_tag="PLC06_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO89420.1"
                     /translation="MSLVAPFALALGLLAVPIILLYMLRLRRREQLISSTLLWQNLVQ
                     DRSANAPWQKLRRNIFLLLQLLILAALTLALARPYILSTDPVTGHVIVLLDASASMTA
                     VEPATGGQSRFEKAVDLVNQIADGNGGTDQMTLIIVNDSPTVAAAATNDHVLLNSALE
                     AVTPSLTGADWHAAFALANGLLQGTGDSRVMIVSDGGLPKDLPPLTGESIFVPVGEAS
                     DNLAITALGSRASAGDTELLVGAANYGSADSVGLLSIYLDGQLYDSRRVEIAPEEEIT
                     LSWTLPADAASVEANLSPIEGNADYFAADNQAWTLPASKSQTSVKLITDGNQFLERFF
                     AIYPQLEVTRVNANSPDSAGEERSHDIYIFDGVAIPSALPAGNILIFDPQPATSQEIN
                     SQISVSEAFTNTQLIELADDPRLANVSWNDVNIAQARQVQGAGLMPLIESDGGPLLLA
                     GEIDGRRIAVFPFDLASSDLPLRIAFPVIMANIIEWLHPGDAAGIDGNINPGAVVPLI
                     PHPRAESVRVEMPNGETWTHSLDGNSEPVLFDQTMLPGLYSVDLYGKSGELVQSGQFA
                     VNFTNPGESQIAPGEAIRVGNIEVLPDNEETRGKSELWAIFLAVGLLLLAFEWWLSYH
                     RGLNRLLLKTR"
     gene            complement(14801..15730)
                     /locus_tag="PLC06_02885"
     CDS             complement(14801..15730)
                     /locus_tag="PLC06_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRO89421.1"
                     /translation="MRLFDEASLHKLEQLTLQADSVRVGAMKGNRRSRKRGTSIEFAD
                     YREYSQGDDLRRLDWNVYARLERPFLKLTEEEEDLAVHILVDVSGSMDWPILEGEEDK
                     PFNKLRYGLTLAGATGYLGLLSGDVVFVTLFDDTYRQSWGPFRGRQNGWPLLQFLENN
                     YERLANRPPSERKQSISINAPLRDYALRGRRPGLLFFLSDLLAADGYREGLSALGSRG
                     HEIVLLHLHSPDEISPQLSGDLRLVDVETGESAEVSLDALTIEAYSDRLAAWHAEIGA
                     FCANRTIHYIPINTNTPWDVIVMQGLRRQGIIR"
     gene            complement(15743..16747)
                     /locus_tag="PLC06_02890"
     CDS             complement(15743..16747)
                     /locus_tag="PLC06_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRO89422.1"
                     /translation="MDNQTLDLDLEKFHDVSSAIVAEVGKVIVGQHDVIRHVLLGILS
                     NGHILMEGVPGLGKTMLIRALGKSLTLEFSRIQFTPDLMPADITGTEILEDSGDGRRA
                     FRFQHGPIFANLILADEINRATPKTQSALLEAMQERTVTAANTTYTLPAPFFVLATQN
                     PIEQEGTYPLPEAQLDRFMFKVNVPYPSTAELTEILARTTTQVEPSLQPVASGEQLLS
                     MQALARRVPVPTNVADFVSRLVIATHPGQSPSSLVNSYVRYGASPRGAQSIILGAKAL
                     ALIDNRFNVSFDDVFAVAPAALRHRLLLNFEGQAEGVGLDTVIADLLTSMPTDSRVGA
                     "
     gene            complement(16797..>17139)
                     /locus_tag="PLC06_02895"
     CDS             complement(16797..>17139)
                     /locus_tag="PLC06_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO89423.1"
                     /translation="VAFLLRFLFTYDQVQQPISKLSGGERSRLQLARLVLERPNLLLL
                     DEPTNNLDIPAIEVLEETLNEFVGTMLVISHDRYFLDQAVDRIIELRDGVLTEYIGGY
                     TDYLDERGYEE"
     assembly_gap    17140..17239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17240..18592)
                     /locus_tag="PLC06_02900"
     CDS             complement(<17240..18592)
                     /locus_tag="PLC06_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRO89424.1"
                     /translation="MAEIIVKLDHISLALAGRLILNDLTWEIQRGQRIGLVGANGAGK
                     STLLKMIAQEIEPDSGSIFRLSGMTIGRLEQEPRLTAGRSVLEEGLYARPEIAAIEQE
                     LTALADKMAKPEIYGDAKLLERSLRVQEKLLHQFEQLGGTRYESKVKETLTALGMGPE
                     KWSTLTEHLSGGQKKLIMLAKLLVLQPPLLLLDEPDNHLDLPAKRALETIIRGYPGCV
                     VIISHDRYLLDEVATQIVELENGRVTQYNGNYSAYANERAIRRLRQQQLYAAQQKEIA
                     RIEAAIARFELWASLVVNERHIRQARSRQKMLDKMDKIEKVTEAKKMTLDLSGWRGSN
                     KVLELAGVGRTFDNGHRLFSDLNTVIWHGERVGLVGPNGAGKSALFKMILDPDSATTG
                     EIKLGPSIKIGYYAQEQETLDPAQDLITCIRQAAPLTQESAVAFLLRFLFTYDQVQQP
                     I"
BASE COUNT         4017 a         5116 c         5274 g         4255 t
ORIGIN      
        1 gtccaggatt ttgcctatca gtataaccta acacaccgaa ctcgtcagtt attggggcac
       61 aatttttaca ccagcaatcg taacgggttt tccagcaatt gtttgaagcg ttgcatatat
      121 tgagctgctt ccgcgccatc ggtcacgcga tggtcggccg atattgtggc tttcatgcgt
      181 gtaccgatgg ccaattggcc atcttttacg accggcacct tccgggccga cccaatcgcc
      241 aggatagcgg cttcgggcgg gttgataatg gcgatgaaat gatcgacttc atagacgccc
      301 agattgctga cggtaaacgt gccaccctcg acatcatcag gccgcacctt tccatcgcgc
      361 gcgcgggcga tcatctcgcg attgtctttg gcgaccttcg agagggtgga tgtgtcagtg
      421 tctttctgga cgatggttgt caacccgcct tcgacggcca cggccgaacc gatgttgatc
      481 cgcttgtgac gcactatctt gtcacccgat agctcggcat tcaggttagg gaaatcccgt
      541 acagccaggg ccgcagcctt cacgatgatg tcattgactg ataccttggc gtcatcgggc
      601 agcgtggcgt tgatctgttt gcgcaaatca agagccgctt ccatgtcgat ctccgtcgtc
      661 acatagaagt ggggcaccgt cgtcttgctt tccgtcatgc gacggccgat ggcctggcgc
      721 aaacgtgtca gggcgatagc ttcgctctcc gggccgattt ccaccggcga ggctgttgga
      781 acttgcacgg gggtagcgac gggtttgggt tgtggcgcgg ccacgaatgc ctcgacatcg
      841 gccttggtta ctcgtccgtc cggcccactt ccctttacct ggagcagatt gatctgcttt
      901 tcccctgcca ggcggcgggc cacaggcgtg gcttttaccc caccagggta agattcatcc
      961 ggcttggcgt cgtttgtggc gacttcggta gtggatggtg cggagacgga aacttcactt
     1021 ggtgcttgct tcgtcacttc ttcggctgct accggcgctg ctgcgacggc cgctgcctga
     1081 gagccattgc cgccggcaag cccggaaatg tcttcacctt gttggccgac gatggccata
     1141 ttcgccccaa caggcacaac gtctcccgcc tcgtgcaaca gatggagcaa tgtgcccgcc
     1201 acatgtgatt caagttcgag cgtggcttta tccgattcga tctcggcaac gatctcacct
     1261 ttgctgaccg gatcgccaac cccttttagc catctgttca ggacgccttc gcgcatatca
     1321 aagcccagtt tgggcatgac gatataatcg gccattagag tatctccttc accgccgcaa
     1381 tgacgcgatc tttgttaatg agcgccgatt gttccagttc acgggaatag gggagcggca
     1441 cttcgaattg ggcaacgcgt ttgattggcg cgtccatata gtcgaacgcc tcttcctgca
     1501 ggcgtgcggc gatctcggcc cccacgccgt aagttcgatg accctcttcg acaatgaccg
     1561 cccggaaggt cttgcggaat gattcaagga cagggcccat atccaacggc cgcaacgaac
     1621 gcaaatcgat gacctctgcc tcgatgcctt ctttggcaag ctggttcgcg gcttcgagtg
     1681 cgacctgcag gccctgggca taggctacga tagtgacatc gctgccttcg cgtttcacgt
     1741 cggagacgcc gatcgggact acatagtctc cttcgggcac ttcgccacga atttgataga
     1801 gagtgtggtg ttcaatgaac agcaccggat catcgctgcg aatggcagcc ttcaacaggc
     1861 ccttggcatc ataaggcgtg ctcggtgaga cgaccgtgaa gccgggaaaa tgggcgaaga
     1921 tgacatccgg cgtgtggctg tgggttgcgc ccagctggcg gccgccgccg cccggagcgc
     1981 ggtaaacgac cgggcattta atctgaccgc cgaacatata atgaactttg gcggcgtgat
     2041 tgatcatttg atccagagcc agaaaagcga aattgatggt cataaattcc gccaccggcc
     2101 gcagaccatt catggccgcg ccattcgcca cgccaccaat agccatctcg gaaataggtg
     2161 tgtcgcgcac acgtcgagcg ccaaattcat cgtagaaccc acgtgtaacc gcataggttc
     2221 caccccatat tccaacttct tcgcccatga tgaacacccg ttcatcgcgt tgcatttctt
     2281 cgcgcaaggc gtcggtaatc gcctgacgca tcgtgatcat tgccatgctg gtacctcttt
     2341 tgctacgctc gcattattgc cgctcgactt tgcgatcgac ataaacatct tcatagagtg
     2401 cttccggatc gggaaaaggt gatttctcgg cgaattcaac ggcttcatcg attacctcat
     2461 ccacctcggc gtcgatcttt tccagcttgc cctttgtagc cgcctttttc tcatagatga
     2521 tgcgttccag gacgccgatg ggatcatctt cgcgccattt ctcgacttcg ctctttgtac
     2581 gatagcgaag cggatcgccc atgctgtggc cttcgaagcg ataagtaatg atctccagga
     2641 attgcggccc cttgccctgg tggatagcat caatggccgc tttggtcgcg tcgtagacct
     2701 caatcacatt catgccgtcg acctttttgg gttccatacc gtaggccagc gccttggtcg
     2761 acatatccgg cacggccgac gcgcgatcga ccgatgtccc catcccgtat tgattgtttt
     2821 caacgatgta gataatgggc agctcccaca cgccggccat attcagggct tcgtggaaat
     2881 aaccgatgtt ggtcgagccg tcgcccatgt agcatagaac gactgcatct gtgcctttgt
     2941 attgttcagc caatgctatt cccgtcccca gcgggacatg accgccgacg atgccatagc
     3001 cgccccagta gttatgggcg acgtcggcca tgtgcatcga gccgcccttg cccttgctca
     3061 ccccggtcgc cttgcctaac aactcagcca tcagggcttt ggggtcggag ccaacgacca
     3121 acgcatgggc atgatcgcgg taggcagtga tcacatgatc ctgtttgcgg cgcgcgccaa
     3181 tcgagccgac ggccacggct tcctggccaa tatagagatg caggaatccg ccgatctttc
     3241 ccatttggta aagttcagca catttttgtt caaaccgacg aactagcacc atttcccggt
     3301 accattccag taacgtcgcc ttttccattg atttctcctt caaaacggtg gtaaaccgca
     3361 gtttggcttt tcccagttga ggttatgaaa agtttagcaa attaatcaga caatggcgac
     3421 tcttagcaag tgcgggcctt tcaggagaga tgggcgcggc caggatcatt ctccatcgcg
     3481 aaacacacat gccccttgcg ctgtcggttt aagcccccac gtcctgctgg ccaatccggc
     3541 cgcggtgaag gcgctttgca tcgcttcgct gatgacaacc tgatggttgt cgcaaaaagc
     3601 gagcagactc ggcccggccc cgctcagggc gacgcccagt gcgccggcct cataggcggc
     3661 gtggtaagca tcgaccgcgc cagggatgag tcggagcctg aagtgctggt gcagacggtc
     3721 gctcatggcc ggtttcagga gggagaactc gggtgccgtt agcgcatgta tgaggagccc
     3781 taaccggctg gtgttgaaaa ttgcgtcccc acgcgagaca gtcggaggca gcacggctct
     3841 ggcttcgctt gtgagcaatt tgaagtccgg caacacgacg acggccgaca ggttgggcgg
     3901 gtcccactgt ctgacgatga gttcatcggg gccgctttcg gtatccggca gaacgccaag
     3961 aaccaatcca cccagcaagg ccggagccac attgtcgggg tgtccttcga tgtccacggc
     4021 catgcgcagg agttcattgc gggggatcgc gtggtcgaaa atagcgtttg cccccagtaa
     4081 tccggcgatg attgccgacg agctgcttcc caggccgctg ccgaccggaa tgccgttgac
     4141 catccgcata gtcaatcgcg tgggccgata gccgattcgt gcaaacagca actcggtcgc
     4201 ttcaatggcc aggttttgtt tgttggcggg caccttttcc tcatcgacgc cgccgacctg
     4261 tatggtgtag tgggtttcac cggcaccata ttcaagaacc ggcccagtgg caaggagggt
     4321 cagctcattc ctgagttcca gggcaagccc caggcaatcg aaacctggcc caaggttcgc
     4381 gctcgttgcc ggcacggtga ccgttatcgg gcgcagaaat agagcagggg acgatgacat
     4441 ggacaacgtt accaggctgt tatggagatc aggcttctgt gggagccgaa acaggtgaca
     4501 agatcgtgtc acccagctgg acggccgcct gattgtgctc gtagtcgagt acattttcaa
     4561 aagcggcgat ggctacagcc agcatatcta tgtccggttc acgggtggtc agatgttgca
     4621 gagccagatt gggtttcgtg ataaggcgga tcagcgcatt accctgatgt gtggccgtga
     4681 acttcaggaa ttcgtaggcg attccagcga taaccggcag caagattagt cgggacagga
     4741 tgcgcggcag aagatccagc ggcgggagga ggctgtagac cagaatcgag atcaccacca
     4801 cgcttagcaa gaacgccgtg ccgcagcgtg ggtgctcgag gggaaaccga gcgactgaag
     4861 ccggggtcag ctccgcgccg gcttcgtagg cgttgatggt cttgtgctcg gccccatgat
     4921 agccatatag cctttttaca tcactcatca atccgatcgc ccagatgtag ccgattagaa
     4981 gtatcaggcg gatggcccct tcgatgaagt tcacaatcac aggggagttt tccaggcctg
     5041 cgagacgctc gatcagatgc gccagaaatg tcggcaagac gaagaatagc aggatggaga
     5101 gggtcagcga gaacacgacg agagtccatt gaaccgggcc ttcgaagact ttctccggtt
     5161 tcccgtcggc gtcttttccg gcctcttctt gcagggcaac ttcggccgcg aagagcaggc
     5221 tcttgattcc cagaccgagc gcgtcccaga gcaaggtcag gccgcgcaag aatggaatct
     5281 tcgccagacg gccgccgtag attcgcggat cgagaggttc cgtatggact acgatgttac
     5341 cctgcggatc gcgcacggcg acggatagcg cgcgagatcc gcgcatcatg acgccttcga
     5401 tgaccgcttg cccgccgtaa ttgacggaag tgaattgagt tggattggga gcatccccat
     5461 tcgtagatag ttgattcgtc agcattgatt aaattgtacc ccgacgactc aagcccgacg
     5521 acacgtgggc ggccgacgct gatcagacgt taactcagag aagggcgatg ataatgagca
     5581 ggatccagcc gatcccaaga tagatactca accagccgag cgcgccacca aacccggctt
     5641 tgccgcggcc gctttgttcc agaacagcgg gaaggttgag gtaccaggaa gctcgctggc
     5701 cctggcgcaa ggccgcaaat cccaggagca ttgccggcag aatgcccaat acgactgcca
     5761 gggcgatggt caagggcgtc aaggttgccc ggatgctatc gagcagactc gcctcacctt
     5821 gcgacagaag tgcgggcaat actaatacca acagcggaac tgcattaagt gccaatccga
     5881 tcaggccata ggtgaggctt gagttggccg acttgccggc tttttcgcgc atttccggct
     5941 gaacagcctc aagcgtgagc gggcggccgc aattggggca aaaagcactg ccgcgcaacg
     6001 ttggcgcata gcagacgttg cacattgtca caaaccccga gcgatctgtg aagacttcgg
     6061 gatccacttt taattccagc agcatatttt gacgattctt gacaagaacg ccggggttat
     6121 cgggaatggc aattggcgcg tccatcgatg gcccaacagg ctgtgccggg tcatccgtgg
     6181 ccacaagagc cgagggacct tcttccacga cggcaggaag tgacgtcgtc gcgctgccgg
     6241 tcaagagtcg ccgagctttg ttgctcatct cctgccgttt ggctttactc gcttgcattc
     6301 gctggagatc gacccactgc atggtggcat tagcctgaac ccgaccacca tcacgcacat
     6361 aggaagcgat caattgcaga agctcgcgag ctttgtcgaa atcgggtgcg tccagctcca
     6421 gttggcttgt gccttcgaac gtccgtacgc gtgccacgac ctcttgtttt tgcaggccca
     6481 tccatttgga aacggtggcg acgataacgc cactctcttc attgtaggtc tgcaatgttg
     6541 cccccatatt ggccagggca aacagggtgc tgcgcatcac gactgtggaa ctgactggaa
     6601 atgctcttac ttctgtgacc gtcatagtga gcgagattat atatgctgtc tccgtaaact
     6661 gtccaaaacc cagaccgttg ggtctgtaat agggcaatgt cgcagaactc aggcttgttg
     6721 atgcgcattt gattcagcat aatccagggt tggccggtga tgttagcggc cgagggattc
     6781 gatcatgtca tttttcaagc gagacaggac tcagaccgat gacgaattta tagatgtcgc
     6841 cggcactgaa agtgttcggg aagggcagat gttgacggtc gaggttcaga agcggaagat
     6901 tatcctgact cgtcatgccg agcgagtcta tgccatcgat acgatatgtc ctcatgctgc
     6961 ggccgatctg agcaagggca gcctgcgcag ctggcaaata tgctgccatg agcatgatta
     7021 ttgcttcgat attcgcaccg gccggatcgt ctggccggaa gatgaggtct atcgcctgag
     7081 gcgatttgag gtgaaggaag agaacggccg cgtcctggtc cgcttatcga ccgtatagaa
     7141 aaaatgataa atgcccgaaa ttggcgttct tcgggcatct attgagtagg tggggtgctg
     7201 ctccctggct tattcggacg gggtgatgtg atagattgcc ccattcgcat aattggccac
     7261 gtacagttca ccatttacat cttcaccgaa cgtggaaata atgaggtccg aatccaggat
     7321 ggcatctgcc agccagccat caccttgggg aaacaggcgc caaataactc ccgaacaaaa
     7381 gtcgctgacg aagtagttgc cccaaagttc aggatacgtc tgtcctcgat agatataccc
     7441 ccctgtgatt gagcaaccct gtgaatgatc gtagtcaaat atgggtagag tcaggccggt
     7501 ctggtcgcag gtgtcggctt caaagcagtg attgccttcc atgatgttcc agccgtaatt
     7561 ctcgccgccc gggctggtgg ccggctggaa gttaacctct tcccatatgt tttgtcccac
     7621 atcggcgatg tagaagtccc ccgtctcgcg atcgaacgaa aatcgccagg gattgcgtag
     7681 gccaagcgcc cagatttcag gctgtgcatc cggcgtgttc aggaaaggat tatcggcggg
     7741 aatcgcgtag gcgttggccg tggaatcaac atccagccgc aagactttgc ccagaaaggt
     7801 cgatagatcc tgacccgcga gcaagggatc attggcgctg ccgccatcgc cgagtcccgc
     7861 ataaaggtag ccatccggcc cgaacgccag gcttccgcca ttgtgattcg gataaggttg
     7921 tttttgcgcg aggtattcta cctcgctgcg aggatcggca cgatagggat cgtccatcat
     7981 caccctgaat cgcgagatat gggtgttccc gctgtaatca gtgtagttga cgaagaactg
     8041 accctgcatc accgcgccat tcgtcgcgta gtcgggatgg aacgccaatc caaggagacc
     8101 ctgctcgctg gaagatgagc cgacgcggtc ggtaatgtcg agaaatggct gttccaggag
     8161 ttggccatcc tcgatgatgc ggatacggcc gacctgctcc agcacaaaca atcgctcgtc
     8221 gaatgcatgg gtgatcccgg taaccatttc cagcccttcg gtaatcacag gttccaggct
     8281 gatcgcggag acgggacgtg caggaatgtc tgtaggctcc ggcgcttctt ccgtggctgt
     8341 tttggttggc gtagtcgctg gttctgtcgc tacttcagtc ggctttggag cttcagccgg
     8401 tgaggtgaca tttgtttcga cggtgacaac aggggagggc aacgtcgtcg gctgggcaac
     8461 tgtcgttggc tcctgagtcg gagcaggcaa agactccttc gccccacacc cagccaatag
     8521 gaaccccgcc accaggaaaa gagctaatat attcttgtgc atattcgcaa ttataaaaac
     8581 cggcctggca attgagtatg ctacaggccg gttctcatat ccttttgatt ttgctatctg
     8641 ccgcagtatt tatactccgg ctaataatct agcggtcggc aagagccacg tactggcggc
     8701 ctcgctcgcc gacatagccc tctttcgggc ggatcaggcg attatcggcc agttgttcca
     8761 gcacctgcga gcaccaaccc gcgatgcggc tcatggcgaa aatgggcgta aaggtatcga
     8821 cgggcaagcc gattgtgtac aacacaacgg ccgagtaata gtcgacgttg gcatacaggt
     8881 cgcgctcgat gaagtagtcg tccttgcggg ccagttgctc aatctctcgg gcaatgtcga
     8941 accacttgcc cgtgccactg ctctcggcca acttttgggc caacggccgc aggatgcgtg
     9001 cgcgcgggtc ctcaaccttg tagatgcggt ggccaatgcc catgatccgc ttgccggatt
     9061 cgcgcgcgtc gtggaaccat tgctcgacat cgcctcgttg ggcagcgtcg ataaattgtt
     9121 ccatggctcg ctgggtggcg ccaccatgcg ccggcccctt cagtgtgccg atcgcgctgg
     9181 tgatagctga ataaacatcg gtgccggtgc tggtagtgac ccgagccgaa aaggtcgagg
     9241 cattgaaacc atgatccgcc aataagacga ggtaggaatc gagtgccgcg actgcgtcgg
     9301 ggtttggctc cttgccgtca agcatgtaca ggaaattggc tgcctgcccc aggccttgcc
     9361 gtggtggtat tggatcgcga ccattgcgca ggcgttccca ggccgcgacg agcgtcggca
     9421 gaattgcggt cagcaatagg gccttttcgc gggcagcatc ggtcgtgatg ttgtcggcat
     9481 tgcggtcgag cagccccatc gcgctgacgg ccgtgcggag cacagccatc gggtgggcct
     9541 cccatggaaa gtcacgcatg ataatgagta gcggttcagg tagcgctcgt tttgattcca
     9601 gggcggcctt gaaccggttc agttcgttct cattgggtaa cttgtcgttc cacaaaaggt
     9661 agacaacttc ttcgaaggtg gcatttgggc ctagatcgcg gatatcgtaa ccgcgataaa
     9721 tcagctcccc tttggtgccg tcgacaaagc tcgatgaggt tgtcatcaga tcttcttttt
     9781 tggtctcaga catgttaaat actcctgtaa agttattggt aattaacctt attgagcggt
     9841 ggctctcaac cgctctccat attgggcttc atagtattgg cggaattcgc cgctcttgat
     9901 tttccgccac caccattcgt tatttttata ccagagcgcg gtttcgatga tagcttcttc
     9961 cggcgtgtga tccggctccc agcctaacgc ggtgatcttg ctgatgtcga ggctgtaacg
    10021 gcgatcgtgc cccggccgat cggccacatg tcgaatcagg ctacgcgggc ggccgagcgt
    10081 atccagcaaa acctcgacca ttgccagatt ctcgacctcg acgcctgtcc cgatgttgta
    10141 ggtctcaccg atagtcccac gatggaggac cagatcgatc gcctcgcaat gatccaccac
    10201 gtgttgatat tcccgcattt gccgtccgtc gccatagacc ggcagaggaa gagagtctat
    10261 agcattagtg gcgaagagag gaacgacttt ctccgggtat tgataaggac cgatattgtt
    10321 cgatccgcgc gtgatggtga ccgggagatc gtaggtgatg aagtaggcat tgacgagcag
    10381 atccgcgctg gccttgctgg cggcataagg gctgcgtggg tctaccttgt cggtttcctt
    10441 gctcgatttg ccgaacggga tatgtccgta aacctcatcc gtcgagattt gatggtaacg
    10501 ttccaggctg aggctcttgg caacttccag caaggtgtag gtcccatata cgctggtctg
    10561 aatgaatgcg tcggggtcca tgatggaacg atcaacgtga gtttcggccg cgaaattcac
    10621 gatcgtgtcg atatcgtgct ccttgacggc ccgttctact gctgcttgat cacagatatc
    10681 gccgcgaaca aatgaatagc ggggattgga gcttgcatcc agcaggttat cgagattgcc
    10741 ggcataggtc aatttgtcat agacgacgat atggtagtcg ttatgacggg ataacatgta
    10801 atgggcaaaa ttggatccga tgaatcccgc cccaccggta acaagaatat tcttcaatca
    10861 atcctcccca acgactgtgc aggctggttc cgtttccccg cctatttatg gcaggaagga
    10921 atgacccaat gtgacaggtt gtggtcgaaa atacggtaat tatagaaagg tggtcgattt
    10981 ttagcgctcg acccatttag cgagtatatc agattttagc tgatgaattc cgattggtaa
    11041 ataatagcta acccgcaact attcgcttcg ggttgaattg gcaataaagt cctgaatcag
    11101 ttcatgagca atgcttggcg gcgaagggac gacaggtaat gactggtggg taaaccatgc
    11161 cgcttcagcg atttcgatcg gatcgacgcg gatctcgcct gactcatatt cggccgtgaa
    11221 ggcgatcatg agcgagtggg gaaatggcca gggctgactc ccaaaatacc gtatgttacg
    11281 aaccttgatg tcaacttctt cacagatctc tcgcgccaca cactcttcaa gcgtttcccc
    11341 cggttcgaca aacccggcca gcacgctgaa catggcggtc ggaaaccgtt gcgcccgagc
    11401 taacagaatt tccgcccctt gatctgtctt tcttgtcact cgcacgatca ttgccggtga
    11461 caggcgagga tagtcggtca gccggcattg ggggcaaatc ttggagcggt cgacggtttg
    11521 ggttacagtt tcatgcccgc atcgtccaca atatcggctg gttcgatccc aatcaacgat
    11581 ctgcacggcg cggccggcca gccagaagga atcctcatcc agcagcgaat agagaggacg
    11641 gagattttcg aaagcgtagc cgtccggcgc ttcggttgat tcgctgacct ctccacaata
    11701 gcaatggatc gatcccgtgc tgctgttaag cgtacccaaa tagatcggac gttcaactgg
    11761 taggccaata tcactcggag agagaagctg cggtattgcg acatcagtct cgtgccgaac
    11821 aaccagcagc ttgaatcctt tgaagatgaa ccataaacct ggctcgtctg tggctggcgg
    11881 cgtgatattt gctgtgaaag ataggcccat aacaatcagt ttaaggatag ccctcggtcg
    11941 ttggtgatgg cgatggatca atcgggtacc ctgtccctga aggcgtattt gtaggcagac
    12001 cgggggcagg tgtcccttcc agtccgggtg ttgggctggg gccaaaagtg ggtgtgcctt
    12061 ccggtgttgc tccgggtggc agcgttccga tttgccccgg cgtgctgccg ggtgtgaata
    12121 acggcgttac cggcgagagg gtcgggtcct gggtttcggt gggcggcatg gggaaagtcg
    12181 tggccgcatc cagagtcatc gtgggggata gctccgatgt ggcttccagc gttagcgttg
    12241 gtgtgggcga acttggtgtt tccaaaggtg agagtgtggg caacgcctct ccggcgacaa
    12301 cggtgagagc tggggcctga gcgccaaata gcgggtttgg ctccacaatt cgttcaacag
    12361 ccagatacca aatggtcccg tcgggcggct ctttcccaca gccgacaggc ccttggtaga
    12421 cactccatac gccgctcaag gtcatctctg tttccgggtc aagaaggcgc accagattct
    12481 caaaaccgat ggcgttcagt tggagatctt cagccaccag gctccagcgg atcgtcgggc
    12541 cggtgtggtc caggcaatct ggagcctcta ccggggtgaa gacccctgaa acctggattc
    12601 gctttcctcg aaaggccgcc ggatcattat ttaattcaga aaatcccatg agtgaggtct
    12661 ctgaatcgat cgtgttcggg gccgctgtgc gagtgggcag gattgggaac aggtgttttt
    12721 cctctcttcc cttcagagag aagacgagca gtgccagtcc gattaacaca aacagcccgg
    12781 caatgagcgg gattaacaaa gtacgtaatc ctgatcgagc aggtgcaacc actcctctgt
    12841 cttcagaggg tacgtcatca ttgggatcag ggtttggttc cggatatatg ctcatctagt
    12901 cttcaacagg agccggttca gcccgcggtg ataagatagc caccattcga aagcgagcag
    12961 cagtagtcca accgccagaa agatagccca taattcgctt ttgccccttg tttcttcatt
    13021 atcgggtaag acttcgatat tccccactcg aatggcttcg cccggcgcta tttgtgattc
    13081 gcctgggttg gtgaagttaa cggcaaactg accggattgt accaattcgc ctgatttacc
    13141 gtagagatcg acagaataaa gccctggtag cattgtttga tcaaacagaa ccggctcgct
    13201 atttccatcc agtgagtgtg tccatgtttc gccgttgggc atctccacgc ggaccgattc
    13261 tgctcgcggg tgtgggatca ggggaacgac ggctcctggg ttaatatttc catcaatacc
    13321 ggcagcgtca cctgggtgta accattcgat aatgttagcc attatgaccg ggaaggcaat
    13381 tcgcaaggga aggtcggagg aagccagatc aaaaggaaag acggcaattc gtcggccgtc
    13441 aatctctccc gccagcagca atggtccccc atcggactca atcagcggca tcagccctgc
    13501 gccctgaacc tgtcgagcct gagcgatatt gacatcgttc caactcacat tggcaaggcg
    13561 cggatcatca gccagctcga tcaattgggt gttagtgaag gcctcggaca ctgagatctg
    13621 cgaatttatt tcttgcgaag tagccggctg gggatcaaat atcaggatat taccagccgg
    13681 caaggcagaa ggaatggcga cgccatcgaa gatatagatg tcatgcgagc gttcttcccc
    13741 ggcggagtcc gggctgtttg cattgacgcg cgtcacttca agctggggat aaatggcgaa
    13801 gaaccgctcc agaaactgat tcccatcggt aatcagcttc acagatgttt gtgacttgct
    13861 tgcgggaagc gtccacgcct ggttgtcggc ggcgaaataa tccgcgttgc cttcgatggg
    13921 ggacaagttc gcctcgacag atgcggcatc agctgggagt gtccaactta acgttatctc
    13981 ctcttcgggt gctatttcga ctcgccggga atcataaagt tggccatcaa ggtagatgct
    14041 aagcaaaccg acgctatcgg ccgacccata atttgccgcg ccgaccagca actcggtgtc
    14101 accggcgctt gcccggctgc ctaaagccgt gattgcgagg ttatcggatg cctctcctac
    14161 cggcacaaag atgctctctc ccgtcagggg aggcagatct ttcggcaatc ctccatcgga
    14221 aacgatcata acgcgcgaat ccccggtacc ttgcaataaa ccatttgcca aggcaaatgc
    14281 cgcatgccaa tctgcgccgg tcagactggg cgtgactgcc tcgagtgcgc tattcagtag
    14341 aacgtgatcg ttggtcgcgg ccgcggctac ggtcggcgag tcattcacga tgattaacgt
    14401 catctgatcg gtgccaccgt taccatcagc tatctgattg acgagatcca ccgccttctc
    14461 aaaacgcgat tgcccacctg tcgcaggttc gactgcggtc atggacgcgg atgcgtcgag
    14521 caggacaatg acgtgtcccg tcaccggatc agtcgataag atgtacggcc gcgccagggc
    14581 cagggttagc gccgccagga tgaggagctg caaaagaagg aagatgttgc gacgcagttt
    14641 ctgccagggt gcgtttgctg accgatcctg aacaaggttc tgccagagca gtgtgctgct
    14701 gatgagttgc tcccgtcgcc gcaagcgcaa catatagagc agaatgatgg gaacggccag
    14761 aagtcccaaa gccagtgcga acggggcgac gagtgacata tcagcggatg atcccctgcc
    14821 gtcgcagacc ttgcatgacg attacatccc agggagtgtt cgtgttgatc gggatgtaat
    14881 ggatggtgcg attggcacag aacgcgccta tctccgcgtg ccaggcagcc aggcggtcgg
    14941 aatatgcctc aatcgtgagc gcgtcgagcg acacctcggc gctctcgccg gtttccacat
    15001 cgacaaggcg cagatcaccg ctgagctgcg ggcttatttc atcggggcta tgcaaatgga
    15061 gcagcacgat ctcatggccg cggcttccca aagcgctcaa gccttcacga tatccgtcgg
    15121 ccgccagcag atcactcaga aaaaacagaa ggccgggtct tcggccgcgc aaggcataat
    15181 cgcgcagggg ggcattgatt gatattgatt gttttctctc tgaagggggt cgatttgcga
    15241 gcctctcata gttgttttcg aggaactgaa gaagcggcca gccgttttgt cgcccacgga
    15301 acggccccca cgactgccgg taagtgtcat cgaataatgt cacgaacact acatcgccgg
    15361 aaaggagtcc cagataacct gtcgccccgg caagagtaag cccgtaacgg agcttgttaa
    15421 aaggcttgtc ttcctctcct tcaagaatcg gccagtccat gctgccgctg acgtcaacga
    15481 ggatatgaac ggccagatcc tcctcttctt cagtcaattt caaaaacggc cgttcgagcc
    15541 gtgcgtagac gttccaatcc aaccggcgca gatcgtcccc ctggctatat tcccggtagt
    15601 cggcgaactc aattgaggtc ccgcgcttgc gactgcggcg attgcccttc atcgcgccga
    15661 cgcgcactga atcggcctgc agcgtaagtt gctctaattt atggagagat gcctcgtcaa
    15721 aaagtcgcat gggacatcgc tatcaagcac cgacgcgcga atccgtgggc atgctggtca
    15781 gcagatctgc gatcacagta tccaaaccaa cgccttccgc ctgtccttca aagttcaaca
    15841 gcaagcgatg gcgcagcgcg gccggagcga cggcgaacac atcatcaaag ctgacgttga
    15901 aacgattgtc aatgagagcc agagctttgg ctcccagaat gattgactga gcgccacgcg
    15961 ggctggcccc atagcggacg tagctgttga ccagagagct ggggctttgc cccggatggg
    16021 tcgcgatcac cagacgactg acgaaatcgg caacatttgt cggtaccggc actcgccgag
    16081 ccaaagcttg catggatagc agctgttcac cggaagcgac cggttggagt gacggttcga
    16141 cctgtgtcgt ggtgcgggcc aggatctcgg tcaactccgc cgttgacgga tagggaacat
    16201 tgaccttgaa catgaatcga tccagttgag cttccggcag cggataggtc ccttcctgct
    16261 caatcggatt ttgcgttgcc aacacgaaga atggggcggg gagcgtgtag gtggtgttgg
    16321 cggccgtcac tgtgcgctcc tgcatggctt ccagcaaagc ggactgggtc ttgggggtgg
    16381 cgcggtttat ctcgtcggcc aggatcaggt tggcgaagat cgggccgtgc tggaatcgaa
    16441 acgcgcggcg tccgtcgccg gaatcttcca gtatctcggt cccagtgatg tcagccggca
    16501 tgaggtccgg tgtaaattgg atacggctga actcgagcgt cagtgatttg cccagcgccc
    16561 ggatcagcat cgtcttgccg aggccgggga cgccctccat caggatgtgg ccgttactga
    16621 gaatgccgag caatacgtga cgaatcacgt cgtgctgccc aacaatcacc ttcccgactt
    16681 ccgcgactat cgcggatgag acatcatgaa acttttccag atcaagatcg agggtttgat
    16741 tatccataag cattcatcat attagcgcga attatactga attcgatgac ggttggttat
    16801 tcttcatagc cacgctcatc cagatagtcg gtatagccgc caatgtactc ggttagaacc
    16861 ccgtctctta actcgataat tcgatcgacc gcctggtcga gaaaatagcg gtcgtgagat
    16921 atgaccagca ttgtgcccac aaactcgttt aatgtttcct ccaacacttc gatggcggga
    16981 atatccagat tgtttgttgg ctcatccagc agcagcaggt tcggccgctc cagcacaagc
    17041 cgggccagtt gaagccggct tcgctcacca ccgctcagct tgctgatggg ttgctgaacc
    17101 tgatcgtagg tgaagagaaa gcgcagcagg aaggccacgn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnng atgggttgct gaacctgatc gtaggtgaag agaaagcgca
    17281 gcaggaaggc cacggccgac tcctgggtca atggcgccgc ctggcgtata caggtgatca
    17341 gatcctgcgc gggatcgagc gtctcttgtt cctgggcgta ataaccgatc ttgatactcg
    17401 gcccgagctt aatctctccg gtcgtcgcac tgtcagggtc caggatcatt ttgaacagcg
    17461 cggatttacc cgcaccgttc gggccgacca aaccaactcg ttcgccgtgc cagataaccg
    17521 tgttaagatc gctgaacagc ctgtgcccgt tgtcaaaagt tcggccgacg cccgctaatt
    17581 ccagtacctt gttgctgccg cgccaaccac tcagatcgag tgtcatcttt ttggcttcgg
    17641 taaccttctc gatcttgtcc atcttgtcga gcattttctg gcggctgcgg gcctggcgaa
    17701 tgtgtcgctc gttgacaaca agggaggccc ataactcgaa ccgggcgatg gccgcttcaa
    17761 tacgcgcgat ctccttctgt tgagctgcgt atagttgctg ctggcgcagg cgacgaatag
    17821 ctcgttcgtt ggcataagcg gagtaattgc cgttgtattg cgtcactcga ccattttcaa
    17881 gctccacgat ctgggtcgcc acttcatcga gaagatagcg gtcatgagat atgatgacga
    17941 cgcagcctgg gtacccgcgg atgatcgttt ccaacgcgcg tttcgcgggc agatcaagat
    18001 ggttatccgg ttcatcgaga agcaagagcg gcggttgcaa gaccaaaagc ttggccagca
    18061 tgatgagctt tttctgtcca ccactcaagt gctcagtcaa tgttgaccat ttttccggcc
    18121 ccatgcccaa tgccgtcagc gtctctttta ctttgctctc gtaccgcgtg ccgcccaact
    18181 gctcaaattg gtgtagcagt ttttcttgca ctcgcagcga tcgctcaagt aattttgcat
    18241 ctccatatat ttccggcttt gccattttgt ccgccagggc agtcaactcc tgttcgatgg
    18301 ccgcgatttc gggccgggcg tataagccct cttctaacac gcttcgcccg gctgtaagcc
    18361 ttggttcctg ctccagacgg ccgatcgtca tccccgatag tcgaaagatt gagccggaat
    18421 caggctcaat ttcctgagcg atcattttaa gaagggtgga ttttccggcg ccgtttgcgc
    18481 cgaccagccc aatacgctgg ccacgttgaa tctcccatgt caggtcgttg agaataagcc
    18541 tccccgcaag cgcaagacta atgtggtcca gtttgacgat gatctctgcc atcacttatc
    18601 tcctgtaaaa caaaaaagcc ggggctttgt gccccggctc tggaatgatc taagtttact
    18661 ttacataaca ttcattcgtg aggcacaata cgataaggga cagagctggt ggaagccaac
    18721 agatgcaatt tcccaatgcg tcgtgtgtat ccgatgatga aa
//
LOCUS       DAOMQG010000025        18652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1296_length_18652_cov_11.731193, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000025 DAOMQG010000000
VERSION     DAOMQG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18652
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1296_length_18652_cov_11.731193"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLC06_02905"
     CDS             <1..499
                     /locus_tag="PLC06_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRO89425.1"
                     /translation="DHAAEWCEDQLCANQRDDSARKSLLDYLMAIAPQPATERLIGYM
                     EREVLDAGEHLIRQGVESDHLFFLESGQLTARLENPDRPPVRLETMQGGHAVGELGFY
                     MGTRRSAAVVVDRPSVIYVMSKATLDRLEKEDPEAAYIFHRIIIHVLGERVLHLVRAI
                     DALQS"
     gene            complement(515..1159)
                     /locus_tag="PLC06_02910"
     CDS             complement(515..1159)
                     /locus_tag="PLC06_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89426.1"
                     /translation="MIRRTNRWPALAAILLLLLAACSGGAAVVGVEQSAQGATGPQKI
                     ENATQIVKFDPTSIATTGDPVAGTCREWAAMPGTYQCDLANEVTGPCFALDSTQLVCR
                     PDPVAGTYGALVAPGESLPPVVPPSPERTVPFFVELVDGVTCTLRVAPEPVIIGGIEA
                     RYECSQPYTYILGEGDSPFSHDAPLWEAGIYTLDPTTGQSSGKTPVGVRRAWIS"
     gene            1340..2773
                     /locus_tag="PLC06_02915"
     CDS             1340..2773
                     /locus_tag="PLC06_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008415598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRO89427.1"
                     /translation="MNDYQLFIGGEWRDSHSAERIPVIDPATEATIATIPRGNAADID
                     DAVVAARAAFEGPWRRMKPKERARLLYDMARAIEREADAFAELETRDSGKPLQLSLKE
                     TLGTARYFEYYAGIADKLHGEQIPLGPDYVDFTIREPLGVSAQIVPWNMPLNMIGRGV
                     APALAAGNTVVVKPAEQTSLTALKLSDLFIELGFPPGVYNVVTGYGGEAGAALASHPD
                     IDGITFTGSVATGRRIMQAAAEHIKPVVLELGGKSPHIIFADADLDLAAAEVVKGIYS
                     NAGQICSAGSRLLVEERIREPLLERVIARSRQVRVGPGMARPDMGPLVSAEHYERVNG
                     YIEIGRREGARVLTGGGRPADLERGYFVAPTIFDEVTGDMRIAQEEIFGPVLAALTFR
                     DEEEALAIANGTPYGLVAGIFTNDINRALRLAGAIRAGQIYINEYYAGGEETPFGGTG
                     QSGFGREKGLATLQGYTQIKNIAIRLQ"
     gene            2841..5813
                     /locus_tag="PLC06_02920"
     CDS             2841..5813
                     /locus_tag="PLC06_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRO89428.1"
                     /translation="MTITHGFELIREEAIPELNTQARLWRHARTGAQLLSLENDDENK
                     VFGISFRTPPADSTGLPHILEHVVLAGSQKYPLKDPFFELVKSSLATFINAMTFPDKT
                     IYPAASTNLQDFYNLLDVYVDAVFHPLLSPEKLAQEGWHYELEATDSPLTFKGVVFNE
                     MKGAYSSPDNLLGRYSQQSVFPDVTYGVDSGGDPAVIPDLTYEQFKRFHETYYHPSNA
                     LIFFYGDDDPEERLRRMDDYLAGFEPIAVDSAIGLQAPFAAPRVARFPYGVDDDAESE
                     EEGDQPRSFVEINWLLPEYEDTVLVMALEIVTYALLHTPASPLRKALIDSGLGEDVIG
                     GLSDDTRQMTFSAGLKGVDPVDVERVEPLILDTLAALARDGFDPDMIAASVNTIEFAL
                     RENNTGRFPRGLALFIRSLSTWLHDRDPLAPLAFEAPLAAVKERLAAEPGYLQALIRG
                     YLLDNPHRVTVILEPDPQLNQRLETAERERLDRTRAAMSDDEALAVIEAARALKEAQE
                     TPDSSEVLAMLPSLGREDLDKAHKAIPIKVMNLETGFLPRNPVSKDQPKVLYHDLFTN
                     GIVYLDLGLDLRSLPASLLPYAGVFGQALLEMGTDKEDYVKLSQRIGRTTGGIGSDTL
                     ITPVVASAVGDQPTAAYLMLRGKSTPEQAPEMLDILRDVLLTAQLDNRERFRQIVLEA
                     KAGAESGLAPGGHIVTNTRLRAHLHPAYRIEEAVNGVENLLFLRRLAERIDGDWPGVL
                     ADLEATRAALVSRGGLIANVTLDEARYGQFAPRLAEFIDALPKQSVRIEGGPNDLATL
                     PRYEGLAFPAQVNYVGKGGRLYDLGYELDGSIAVINNLLRTGYLLQKIRIQGGAYGAF
                     CTFSATSGIYTYLSYRDPNVSNTLAVYDATADFLRGLELSEDELTRGIIGAIGSIDAY
                     QLPDAKGYTSLTRYLTGETDERLQRFRDQALGTTTADFRKLADALEALKTSGDVVVLG
                     SREALDGAAIDLAMTRVM"
     gene            6059..6940
                     /locus_tag="PLC06_02925"
     CDS             6059..6940
                     /locus_tag="PLC06_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRO89429.1"
                     /translation="MRKIYLPVLIVTALLLAGLAIVTGPAAPAYGQSSAPATPRADGS
                     IVHIVQPGESLWGIAIQYASILGMSAEDALPYLQELNNNPAFINPGDEVIIRQGEPAT
                     ATPEPTIEATVDTAATAEATAPAAEVPPVDLAPTQAPAGTICVAAFDDANADGQRNEG
                     EPLIANAAIALARAGTTTSTYITDGISEPFCFELTEADSYELQVFPPAGFTSTTEDTW
                     AVAIANGESYTVSFGLTTAPAATATPAASDAATPAAGDTTASATGGFGSLGWIVAGIA
                     VVLVLLAVVGVVLLRRG"
     gene            complement(7041..7391)
                     /locus_tag="PLC06_02930"
     CDS             complement(7041..7391)
                     /locus_tag="PLC06_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89430.1"
                     /translation="MLHFLEFMTSFYWPLIIETMFGTFRRARWRKAVARYGPLGLLTE
                     TAESFIGTNTYVLYVPLAGRWDKRSIAKLLKQYGIDMWGWGYWNHELFFHVRRDDAWL
                     AQDILISAGVELLG"
     gene            7563..7919
                     /locus_tag="PLC06_02935"
     CDS             7563..7919
                     /locus_tag="PLC06_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89431.1"
                     /translation="MDWLFARDECWGGMLELFEGFAKLLFNMIFMPLLDSGIYRQGFC
                     AGMLIALAIGWSSRALLAARGKITVFFADVQPSLKSSPSPYKRMNGCLFGLLTFLLFI
                     VTTLTFLWAFTYFLSR"
     gene            8069..10867
                     /locus_tag="PLC06_02940"
     CDS             8069..10867
                     /locus_tag="PLC06_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRO89432.1"
                     /translation="MQTNQQPPVQYVPATPPPARQKRGCGCIGGAILGVIALMLLIVV
                     GGAAAAGGLVYANWSREIESGVASLETARDRETFETTRITDRNGEVLWEIFGEGKRTR
                     IPLSEIPDYFIKATIAVEDDTFYENIGLDAPSLAAAVIANVRNPDARPVGGSTITQQI
                     VRHIAFDYEERTAVSYDRKIKELVLAWMMNRDFTKDEILEMYLNEIYYGNLAYGVEAA
                     ARTYFAKPASALTLGESSLLAGLPQSPVDLDPFTNLEGAKERQWLILNLMVSEGFIDQ
                     ATAETAYLEPLNFAAQEVSLEAPHFAVYVRQLLEQQYGAELVANGGLRVTTSLDMEFQ
                     RLAEGLARQHVGQVDPAKNLTNAALVAMKPGTGEILAMLGSVDYENEAIDGHVNVTLS
                     PQQPGSSIKPLTYAAALSPTDPASPEGGAPRWTAADILWDVPVKYEQAVGEPYAPVNY
                     DGAFHGPVRLRAALANSYNIPAVLLLQDIGVPRLLEFGRALGLDSWTGDSSQYGLSLT
                     LGGGEVTPLELTTAYAALANGGNRVAPVAILRVERTNGEVLYEYQPSEPQRVIDERVA
                     YVISNILDDDQARIPAMGSPNPMELPFPAAAKTGTTNDFRDNWTMGYTPGLVVGVWTG
                     NTDNSPMVHISGLEGAAPLWSSYMQGVYSNGGLVARLEVNGQVPPSEFIRPLGLTERP
                     LCALTSAAVGSIDCVPAGEELFLESGLPPTPMPTVEGPAVFWEKLDPAVWRAVAMPLP
                     PPTPEEIALIPPEQLEDQPPPQLFCHFAEGTALELLPPTAAPQVFLSPPHNAESLKPA
                     HEWAQANNIAILPTATCTEEMLAESLGQGQTAVWRITSPADGDIVKGNVPIIGTASFD
                     PAEVEFYKIELGIPNGGDIQWLTLGETHNTPVVNGQLEYLQAEGLPPGVYYLRLIVIQ
                     DSNYVGEPHQIQIIVEAP"
     gene            complement(10955..12781)
                     /gene="thrS"
                     /locus_tag="PLC06_02945"
     CDS             complement(10955..12781)
                     /gene="thrS"
                     /locus_tag="PLC06_02945"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRO89433.1"
                     /translation="MSDNNIPYIETELYRIRHSAAHVMAEAVVDLFPEVKLAIGPPVE
                     DGFYYDFDLGKDERGKPRTFSPEDLERIEERMKELLRDNAEFQHTTMGIDEALAFFRD
                     QPYKVELIKDLAAGKVDEMGNPTAEPATEVGIYRQRDFVDLCRGPHVGRTRDIKSNAV
                     KLLRTGGAYWRGDEKNPQLQRIYGTAWHNKTELDDYLQRLEEARLRDHRRLGKQLGLF
                     HISPLVGSGLPLWLPKGAVLRETLESFLRQAQLERGYLPVITPHIGNLELYKKSGHYP
                     YYKDSQYTPIKVDEEEFLLKPMNCPHHIEIYRSEPRSYRDLPYRLAEFGTVYRYEKSG
                     ELTGLTRVRGFTVDDSHLFVTPDQLEEEFIAVVDLIQYVFSTIGFSDFRARLGINDPT
                     SDKYAGEPEMWERGIAAIRNAADKLGLNYSVEKGEAAFYGPKLDFIFRDVLKREWQLG
                     TVQVDFLLPERFDLEYTGEDGQKHRPVMIHRAPFGSMERFVGILIEHFNGAFPVWLSP
                     VQVAIIPIADRHLEYARQMAAALKEIGARVMVDDSSERMNRKIREAQLQKIPYMLVVG
                     DKEMEAGAVAVRTRDNEDHGAMSLADFAEKLSSSISERSMEL"
     gene            complement(13261..13941)
                     /locus_tag="PLC06_02950"
     CDS             complement(13261..13941)
                     /locus_tag="PLC06_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89434.1"
                     /translation="MSKTTQALTLSCMFMLSSIGLIACSSREPISGETENDQTQIIVS
                     SEIISLTRDEMIDQADMVFQGLVEEISTTHFNQESGEYLEGGRPFHTITFKVLRPIVG
                     SMDVDQKVTITTWGNSPAEEMVLVQTSEGGAYLHSDAGHDLQPGDIVIVFANQRSTMW
                     REGKRETFSFVGVPFESYFRLQEDGRYLGRPDNEPVEIEELVSRIAEQRTVIESTPIT
                     PREGTPSE"
     gene            complement(14062..15309)
                     /locus_tag="PLC06_02955"
     CDS             complement(14062..15309)
                     /locus_tag="PLC06_02955"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HRO89435.1"
                     /translation="MNFDDDTPLVWLKNPHMDAQAGRKAVDHLLPDGTTEEARAFHMQ
                     MPRFEMTPLKRLSNLASRLGLGDIWVKDESARMRLNAFKALGGSFAIYRFICRKVGRP
                     SLSFEELMSDDIRATLGDITFTSATDGNHGKGLAWATRKFGFPCVIYVHKHTSQARID
                     AIAEFGAEVRVVDGNYDDAVRQCERDALANGWQVVSDTSWPGYEDIPKWVMQGYTTLL
                     SETQEQLGAQGIVKPTHIFLQAGVGAFAAAMIAFYEKLFGAGAPISVVVEPRKADCLY
                     QSLRIGDGRPHSVSGDLDTIMAGLACGEPSPLAWEILRDCADFIVTAPDYVAAKGMRM
                     YGVPLKGDPVVISGESGAVTLGVLPFIMGYEGAAELRQQLQLDENSRVLLINTEGNTD
                     PDYFRNVVWEGVFPVPEPYRWRS"
     gene            complement(16042..16254)
                     /locus_tag="PLC06_02960"
     CDS             complement(16042..16254)
                     /locus_tag="PLC06_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89436.1"
                     /translation="MKKTPTNNPESDDLPAEYEFDYRKARPNRFALKDQERVVILEPD
                     VAEYFHDSDAVNRVLRALIETMPRAV"
     gene            complement(16298..16738)
                     /locus_tag="PLC06_02965"
     CDS             complement(16298..16738)
                     /locus_tag="PLC06_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRO89437.1"
                     /translation="MASWAEFTQAAPELAAFGKTRFQSEVAYLGTVRADGGPRVHPVT
                     PIMGEQLFLFMEPTSPKGKDLGRDARYTLHCSVEDSSGGGGEFYVRGRATLSDDPLIR
                     QEAVRAASYTPQDHYILFVLTVEFAFMNRYVDGNANPRRWRSSE"
     gene            16940..17263
                     /locus_tag="PLC06_02970"
     CDS             16940..17263
                     /locus_tag="PLC06_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89438.1"
                     /translation="MQDRDGNSVYLTEERWHHITEPNNHPEVAEYEDALKMALRRGRR
                     RQEPLSPRKYRYAYPVEGLPDGFNHIVVIVLFGFNVDEQGATIPNNFIATAFLKHIRL
                     KGGVE"
     gene            17260..17643
                     /locus_tag="PLC06_02975"
     CDS             17260..17643
                     /locus_tag="PLC06_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRO89439.1"
                     /translation="MIPEATYTYDYDKEADVLYISFSPSERPTAAVELNDNILLRFNR
                     AEKRAIGLTLMDFSVLVQLTELGPRSFPLSGLADLEPDWQEAVIEIITASPVNRILKV
                     SSYMPTAAEVVPITSVERPPIPLAV"
     gene            complement(17760..18113)
                     /locus_tag="PLC06_02980"
     CDS             complement(17760..18113)
                     /locus_tag="PLC06_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRO89440.1"
                     /translation="MLLLAADENFDNDIVRGLLRRRPDIDIVRVQDVGLSGASDSIIL
                     ELAAEQQRLLLTHDVATITHYAYQRVEANQPMPGVIEVSKSTAIGIVIDDILLLAEFS
                     VENEWEGRIIYLPLR"
     gene            complement(18120..18455)
                     /locus_tag="PLC06_02985"
     CDS             complement(18120..18455)
                     /locus_tag="PLC06_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO89441.1"
                     /translation="MTELITIETIPLETDLNGVIRVSKTRVTLDTIVQAFNDGATAEE
                     IAQQYPSVPLADIYSVIGYYLRNKDEVGVYLHQRRGEAEQVRRLNETRSDPAGVRERL
                     LARQAATEL"
BASE COUNT         3671 a         5738 c         5527 g         3716 t
ORIGIN      
        1 cgatcacgcc gccgagtggt gcgaggatca gctatgcgcc aatcagcgag atgattcggc
       61 gcggaaatcg ctgctggatt acctgatggc gatcgcgccg cagcccgcca ccgaacgact
      121 cataggctat atggagcgcg aggttctcga cgcgggcgag catctcatcc ggcaaggcgt
      181 tgaatccgac catctcttct tccttgagtc gggtcaactg acggcccggc tggaaaaccc
      241 cgaccgaccc ccggtgcggc tcgaaacgat gcagggcggc cacgccgtcg gcgaactggg
      301 tttctatatg ggcacccggc gcagcgcggc cgtcgtcgtc gatcgcccaa gcgtgatcta
      361 cgtcatgtcg aaggcaacgc tggatcggct ggaaaaagaa gaccccgagg ccgcctacat
      421 ttttcatcgc atcatcatcc acgtgcttgg cgagcgggtg ctgcatcttg ttcgcgctat
      481 cgacgccctg caaagctagg ggcggggtgg cgtattacga gatccacgcc cggcgaacgc
      541 caacgggcgt cttgcccgac gactggcctg tggtcggatc cagcgtgtaa atgcccgctt
      601 cccacagcgg cgcgtcatgg ctgaagggcg aatcgccctc gcccagaatg taggtgtagg
      661 gctggctgca ttcatagcgc gcctcgatgc cgccaatgat gaccggttcc ggcgccacgc
      721 gcagggtgca ggtgacaccg tcgaccaact ccacgaagaa cggcacggtg cgttcgggcg
      781 acggcggaac cacgggaggc agcgactcgc cgggggcgac aagagcgcca taggtgccgg
      841 ccaccggatc ggggcgacag accagttgcg tgctatccag ggcgaagcac ggcccggtca
      901 cttcgttggc cagatcgcat tggtaggtgc cgggcatggc cgcccactcc cgacacgttc
      961 cggccacggg gtcgccggtc gtcgcgatgg aagtcgggtc gaacttgacg atctgcgtcg
     1021 cgttctcgat cttttgcggg ccggttgcgc cttgcgccga ttgctcgacg ccgacgacgg
     1081 ccgcgccgcc ggaacaggct gccagcaata ataacaagat cgccgcgagt gccggccatc
     1141 gattggtcct tcgtatcata acactccttc tcagagacca ggcaggcggc gaagcagaat
     1201 tccattccag aacgagggtt aattgcctgc aggcccgatc atactcaatg ccgcctgtca
     1261 ttatatcacg ctcatgggtg ccatctgcta taatccattc cggtcgcact gacagcaaac
     1321 gggcggccgc gaaatcgcca tgaacgatta ccaactcttt atcgggggtg aatggcgcga
     1381 cagccattcg gcagagcgca tcccggtcat cgacccggcc acggaagcca ccatcgctac
     1441 tattccgcgc ggcaatgcgg ccgatatcga tgatgccgtg gtcgccgctc gcgccgcctt
     1501 cgaagggcca tggcggcgca tgaagcccaa ggagcgcgcc cggctgctct acgatatggc
     1561 ccgcgccatc gagcgcgagg ccgacgcctt cgctgaactg gaaacccgtg acagcggtaa
     1621 accgctgcag ctctccttga aggaaacgct tggcacggcg cgctactttg aatattacgc
     1681 cggcattgcc gacaagctcc acggcgagca aattcccctc ggccccgact atgttgattt
     1741 caccatccgc gagccgctgg gcgtcagcgc ccagatcgtg ccgtggaaca tgccgctgaa
     1801 tatgatcggc cgtggagtgg ctccggcgct ggccgcgggc aatacggtcg tcgtcaaacc
     1861 agccgagcag acgtcgttga ccgctctcaa attgagcgat ctgttcatcg aactgggctt
     1921 cccgcccggc gtctacaacg tcgtcaccgg ctacggcggc gaggccggcg cggcgctggc
     1981 gtcccatccc gacatcgacg ggatcacctt caccggttcg gtggccacgg ggcgacggat
     2041 catgcaggcc gcggccgagc atatcaagcc ggtcgtcctg gagctgggcg gcaaatcgcc
     2101 ccatatcatc ttcgccgacg ccgacctgga tctggccgcg gccgaggtcg tcaagggcat
     2161 ctacagcaat gccgggcaga tctgttccgc cggatcgcgc ctgctggtcg aggaacgcat
     2221 ccgtgagccg ctgctggagc gggtgatcgc gcgctcgcgg caagtgcgtg tcgggccggg
     2281 catggcgcgg ccggatatgg ggccgctggt gtcggccgag cattacgagc gggtgaacgg
     2341 ctatatcgaa atcggacggc gtgagggcgc gcgcgtgctg accggcggcg gccgtcccgc
     2401 cgatttggag cgcggctact tcgtcgcgcc gactatcttc gatgaagtga ccggcgacat
     2461 gcgcatcgcc caggaagaga tattcgggcc ggtgctggcc gcgctcacct ttcgtgatga
     2521 ggaagaggcg ctggccatcg ccaacggcac gccctatggg ctggtggccg gtattttcac
     2581 caacgacatc aaccgcgccc tacggctggc cggagccatc agagccgggc agatctacat
     2641 caacgagtac tacgccgggg gcgaggagac gcccttcggc ggcacaggac agagcggttt
     2701 cggccgcgag aaaggtttgg cgacgctcca gggctatacc cagatcaaaa acatcgctat
     2761 tcggctgcaa tgaatgattg catgtcacga gccttaatca caattgcagt aaagaatact
     2821 atccggagca taactgatat atgacaatca cccacggctt tgaattgatt cgcgaggaag
     2881 ccatccctga gctgaatacc caggcgcggc tatggcgaca cgcccgaacc ggcgcgcagc
     2941 tcctctcgct ggagaacgac gacgagaaca aggtgttcgg catcagcttt cgcacgccgc
     3001 ccgccgactc caccggcttg ccgcatattc tggaacacgt cgtcctggcc ggttcgcaga
     3061 agtacccgct gaaggacccg ttcttcgaac tggtgaaaag ctcgctggcg acgttcatca
     3121 acgccatgac cttccccgac aagacgatct atccggcggc cagcaccaat ctgcaggact
     3181 tttataatct gctcgacgtc tacgtcgatg ccgtcttcca tccgttgttg tcgccggaga
     3241 agctggctca ggaaggatgg cattacgagc tggaagcgac cgattcgccc ctgaccttca
     3301 agggcgtcgt cttcaacgaa atgaagggcg cctattcgtc gcccgataac ctgctcggtc
     3361 gctacagcca gcagtccgtc ttccccgacg tcacctacgg cgtcgactca ggcggcgacc
     3421 cggccgtcat ccccgacctg acctacgagc agttcaagcg ttttcacgaa acctactacc
     3481 atccgtcgaa cgcgctcatc ttcttctatg gcgacgacga tcccgaagaa cggctgcgcc
     3541 gaatggacga ctatctggcc ggattcgagc cgatcgcggt tgacagcgcc atcggtttgc
     3601 aggccccatt cgccgcgccg cgcgtggcgc gcttccccta cggcgtcgat gacgatgctg
     3661 aatcagagga agaaggcgac cagcctcgct cgttcgtcga gatcaactgg ctgctgccgg
     3721 agtatgagga tacggtgctg gtcatggcgc tggagatcgt gacctacgct ctcttgcaca
     3781 cgcccgcctc tccgctgcgc aaggcgctca ttgactccgg tctgggcgag gacgtcatcg
     3841 gcgggctttc agatgatacg cgacagatga ccttctcggc cggcctcaag ggggtcgatc
     3901 cggtcgacgt cgagcgcgtg gagccgctca tcctcgacac gctggcggcg ctggctcgcg
     3961 acggctttga tcccgacatg atcgccgcct cggtcaacac tatcgagttc gccctgcgcg
     4021 agaacaacac cggccgcttc ccgcgcggcc tggccctgtt cattcgctcg ctaagcacgt
     4081 ggctccacga ccgtgacccg ctggccccgc tggccttcga ggccccgctg gcggcggtga
     4141 aggagcgatt ggcggccgaa cccggctacc tgcaagccct cattcgtggc tacctcctcg
     4201 acaaccccca tcgggtgacg gtcatcctgg aacctgatcc gcagttgaat cagcggctgg
     4261 aaacggccga acgtgaacga ttggaccgaa cacgtgcggc gatgagcgac gacgaagcgc
     4321 ttgcggtcat cgaggccgcc cgcgcgttga aagaagccca ggagacgcct gactcgtcgg
     4381 aagtgctggc catgctgccg agcctggggc gggaggattt ggacaaggcg cataaggcga
     4441 taccgataaa agtgatgaat ttagaaaccg ggtttcttcc gagaaacccg gtttctaagg
     4501 atcaaccaaa ggtcctctac cacgatctgt tcaccaacgg catcgtctac ctcgatctgg
     4561 ggctggacct gcggtcgctg cccgcttcgt tgctgcccta tgccggtgta ttcgggcaag
     4621 cgttgctgga gatgggtacc gataaagagg attatgtcaa attatcccag cgcattggcc
     4681 gcacgactgg cggcattggc tccgacacgc tcatcacccc ggtcgtcgct tcggccgtcg
     4741 gcgaccaacc aacggccgcc tatctcatgt tgcgcgggaa atcgacgccg gaacaggccc
     4801 ccgagatgct cgacatcctg cgcgatgtgt tgctaactgc ccagctggac aaccgcgagc
     4861 gctttcgcca gatcgtgctg gaggccaagg ccggtgcgga aagcgggctg gctccgggcg
     4921 gccatatcgt gaccaacacc cgtctgcgtg cccacctcca ccccgcctac cgaattgagg
     4981 aagcggtcaa cggcgtggag aacctgctct tcctgcggcg gctggccgaa cgcatcgacg
     5041 gtgattggcc gggcgtgttg gccgatctgg aagcgacgcg agccgctttg gtgagccggg
     5101 gcggcctcat cgccaacgtc acgctggacg aggcgcgcta tggtcaattc gccccgcgcc
     5161 tggcggagtt catcgacgcc ttgccgaaac aatccgttcg catcgaggga ggccccaatg
     5221 acctggcgac cttgcctcgc tatgaagggc tggctttccc ggcccaggtg aactacgtcg
     5281 gcaagggcgg ccgtctctac gatctgggct acgaactcga cggctcgatc gccgtgatca
     5341 acaacctgtt gcgaaccggc tacctgttgc agaagatccg cattcagggc ggagcctatg
     5401 gcgcgttctg caccttcagc gccacgtcag gcatctacac ttatctgtcc tatcgcgacc
     5461 ccaacgtaag caacacgctg gcggtctacg acgcgacggc cgatttcctg cgcgggctgg
     5521 aattgagcga agacgagttg acgcgcggca tcatcggagc catcggctcc atcgacgcct
     5581 accagttgcc tgacgcgaag ggatacacct cgctgacgcg ctatctgacc ggcgagaccg
     5641 acgagcgctt gcagcggttc cgcgatcagg cgttggggac gacgacggcc gacttccgca
     5701 agctggccga cgcgctggaa gcgctgaaga cttcgggcga tgtggttgtg ctcggcagtc
     5761 gtgaggcgct cgacggcgcg gcaattgatc tggcgatgac ccgcgtgatg tagggtcgac
     5821 attcctgtct gccagattag tgagacagac attcccgtct gtcaggttgc ccgtggagca
     5881 gacaggaatg tctgctctac taacctggtt ctcatgcgcc tttcttattt gtggtatacg
     5941 gtgggaactc ctgttaagat agcgagctgt ctgtccgggg cgcgctgcgc ctcacatttg
     6001 tccgtcgcag acgaaaatcg cgcgacgggc agcccatgtc aggagaatga gaactaaaat
     6061 gcgcaagatt tatctgccgg ttctgattgt gacagctctg ttgctggcgg gcttggcgat
     6121 tgtaaccggt cccgctgctc cggcttacgg tcaatcatcc gctccagcta cgccgcgcgc
     6181 cgacgggtcg atcgtccaca tcgtgcagcc gggcgagtcg ctgtggggca ttgccatcca
     6241 gtatgcgtcc atactgggca tgtccgccga agacgcgctg ccttatcttc aggagctcaa
     6301 caacaacccg gcattcatca atccgggcga cgaagtcatc atccgccagg gcgaaccggc
     6361 cacggccacg cccgagccga ccattgaagc cacggtagac accgccgcga cggccgaggc
     6421 gaccgctcca gccgccgaag ttcctccggt cgaccttgcg ccaacccagg cgccggcggg
     6481 gacgatctgc gtggccgcct tcgatgacgc caacgccgac ggccagcgca acgaaggcga
     6541 accattgata gccaacgcgg ctatcgcgct ggcccgtgcc gggacgacga cctccaccta
     6601 tattactgac gggatcagcg agccgttctg cttcgagttg actgaagccg actcctatga
     6661 gttacaggtc ttcccgccgg cggggttcac ctcgacaacc gaggatacgt gggcggtggc
     6721 cattgccaat ggcgagtcct acacggtgtc gttcggactg acgaccgcgc ccgccgcaac
     6781 cgctactccg gccgcaagtg acgcggcaac tccggccgca ggcgacacga cggcatcggc
     6841 cacgggcggg ttcggaagcc tgggctggat cgtagccggc atcgccgttg tgctggtgtt
     6901 actggcggtg gtcggcgtcg tgctgctgcg gcgcggctaa tcaatcgggg gcgttagcaa
     6961 ggcgcggcac actcttgatt aagcgtcgtg tctgcacagg caagcaagag aagagaggtc
     7021 atgagggcct ctctttttta ttagccgagc agttccacgc cggcggaaat aaggatgtcc
     7081 tgggccaacc aggcatcgtc gcgccggacg tggaagaaaa gctcgtggtt ccagtagccc
     7141 caaccccaca tgtcgatgcc gtattgcttc agcagtttgg cgattgagcg cttgtcccat
     7201 ctgcccgcca gcggtacgta aagaacatag gtgttggtgc cgatgaagct ctcggccgtc
     7261 tcggtcagta atcccagcgg gccataacgc gcaacagcct tccgccatct ggcgcggcga
     7321 aatgtgccga acatcgtctc aatgatcagc ggccaataga agctggtcat aaattccaaa
     7381 aagtgaagca tggcaagtca tctctcaata aagaacatat gttctggtat tgtagatgac
     7441 ttatacagat tgtcaaaagt gatttatgtg ggttatcaaa cttaaaatat tcgatttggc
     7501 cccgcgtaac ccgcattaat agcgataaaa aacggctacc ctgttataat tggtttgttc
     7561 cattggattg gctgtttgcg cgcgatgaat gttggggtgg gatgctcgag cttttcgaag
     7621 gtttcgccaa actacttttc aatatgatct tcatgccgtt actggacagc ggcatttacc
     7681 ggcaagggtt ttgcgccggc atgttaattg cgcttgcaat cggctggtcc tcacgggcgc
     7741 tcctcgctgc gcgcggcaag attacggtct tctttgccga tgttcaacct agtcttaagt
     7801 cttccccaag tccctacaag cgcatgaatg gctgtttatt cggcctgttg actttcctct
     7861 tgtttatagt aaccaccctc actttcttgt gggcgttcac ttacttcctg agccgataaa
     7921 tcgctccccc gtgggggaac aggaatccgg ttttggccaa tcgtcgcctt agcatcacct
     7981 aaacgtttcc cgaccgcatg gcaacccctc gcggattctt ctacactgga tgtggaacta
     8041 atagagtgcg attattcgtc ttatacctat gcagaccaac cagcaacccc ctgtccaata
     8101 cgtccccgcc acgccgccgc cggcccgcca aaaacgcggc tgcggttgca tcggcggcgc
     8161 cattctgggc gtcatcgccc tcatgctgct cattgtcgtc ggtggcgcgg ccgccgccgg
     8221 cggtctcgtc tacgccaact ggtcgcgcga gatcgaatcc ggcgtggcca gcctggagac
     8281 cgcccgcgat cgcgagacct tcgagaccac ccggatcacc gatcgcaacg gcgaagtgct
     8341 gtgggagata ttcggcgagg gcaagcgcac ccgcatcccc ctcagcgaaa tccccgacta
     8401 tttcatcaag gccaccatcg ccgtggaaga cgacaccttc tatgagaaca tcggcctcga
     8461 cgcgccctcg ctggccgcgg ccgtcatcgc caatgtgcgc aaccccgacg cgcggccggt
     8521 gggcggcagc accatcaccc agcagatcgt gcgccacatc gctttcgatt atgaggagcg
     8581 cacggccgtc tcctacgacc gcaagatcaa ggagcttgtc ctggcctgga tgatgaaccg
     8641 ggatttcacc aaggatgaaa tcctggagat gtatctcaac gagatctatt acggcaatct
     8701 cgcctacggg gtcgaggcgg ccgcgcgcac ctacttcgcc aagccggcgt cggcgctgac
     8761 gctgggcgag tcgagcctgc tggccggcct gccgcaaagc ccggtcgatc tggatccgtt
     8821 caccaatctg gaaggggcca aggaacgcca atggctgatc ctcaacctca tggtcagcga
     8881 ggggttcatc gatcaggcca cagccgagac cgcctatctg gagccgctca acttcgccgc
     8941 gcaggaagtc agtctggaag cgccccattt tgccgtttac gtgcgccaac tgctggagca
     9001 acaatatggc gcggagttgg tggccaatgg cggcctgcgc gtgacgactt cgctggatat
     9061 ggagtttcag aggctggccg agggcctggc ccggcagcat gtggggcagg tggacccggc
     9121 caagaatctg accaatgccg ctctggtggc catgaagccg ggcacgggcg agatcctggc
     9181 catgctgggc agcgtggact acgagaacga ggccatcgac ggccatgtca atgtgacgct
     9241 ctcgccgcag cagccgggca gcagcatcaa gccgctgacc tatgccgccg cgctgtcgcc
     9301 caccgacccg gcatcgcccg agggcggcgc gccacgctgg acggcggccg acattctgtg
     9361 ggacgtgccg gtgaaatatg agcaagccgt cggcgagcct tatgcgcctg tcaactacga
     9421 tggcgcgttc cacggcccgg tgcggctgcg ggccgcgctg gccaacagct acaacatccc
     9481 cgccgtgctg ctcttgcagg acatcggcgt gccgcggctg ctggagttcg gccgcgcctt
     9541 gggcctcgac tcgtggaccg gcgactccag ccagtacggc ctgtcgctga ctctcggcgg
     9601 cggcgaggtg acgccgctgg agctgacgac cgcctacgcc gcgctggcca atggcggcaa
     9661 tcgcgtcgcc ccggtggcca ttctgcgcgt ggagcgcacc aacggcgagg tgctctacga
     9721 ataccagccg agcgagccgc agcgggtcat cgacgagcgc gtggcttatg tgatcagcaa
     9781 catcctcgac gacgaccagg cccgcatccc ggccatgggc agccccaacc cgatggaact
     9841 gccctttccg gccgcggcca agacgggcac caccaacgac ttccgcgaca actggacgat
     9901 gggctacacg cccgggctgg tcgtcggcgt ctggacgggc aacaccgaca acagcccaat
     9961 ggtccacatc agcgggctgg agggcgcggc cccgctgtgg agcagctaca tgcagggggt
    10021 ctacagcaac ggcgggctgg tggcccggct ggaggtcaac ggccaggtgc cgccgtcgga
    10081 gttcatccgc ccgctgggcc tgaccgagcg gccgctctgc gccctgacca gcgccgccgt
    10141 cggctccatc gactgcgtcc ccgcaggcga ggagttgttc ctggagagcg gtctgccgcc
    10201 cacgccgatg ccgaccgtcg aggggccggc ggtcttctgg gagaagctgg acccggccgt
    10261 ctggcgcgcc gtggccatgc cgctgccgcc gcccacgccg gaagagatcg ccctgatccc
    10321 gccggagcag ctggaagacc agccgccgcc gcaactgttc tgccatttcg ccgaagggac
    10381 ggcgctggaa ctgctgccgc cgacggccgc gccgcaggtg ttcctgtcgc cgccccacaa
    10441 cgccgagagc ctgaagccgg cccacgaatg ggcacaggcc aacaacatcg ccatcctgcc
    10501 cacggcgacc tgcaccgagg agatgctggc ggagtcgttg ggtcaggggc aaacggccgt
    10561 gtggcgcatc acctccccgg ccgacggcga catcgtgaag ggcaatgtgc ccatcatcgg
    10621 cacggccagc ttcgacccgg ccgaggtgga gttctacaag atcgaactgg gcatccccaa
    10681 cggcggggac atccaatggc tgacgctggg cgagacccac aacacgccgg tggtcaacgg
    10741 ccagctggaa tatctccagg ccgagggcct gccgccgggg gtctactacc tgcggctaat
    10801 cgtcatacag gacagcaact acgtcggcga gccgcatcag atacagatca tcgtggaagc
    10861 cccgtagtac gtcattaaga cctgacaggt gggcgcgatc gggcgcttgc tcgaccggcg
    10921 actttggtgc gcccacctgt caggtctgcc tggattacaa ctccatcgac cgctcggaga
    10981 tggaactgga cagcttttcg gcgaagtcgg ccagcgacat cgcgccgtga tcttcgttgt
    11041 cccgcgtacg cacggccacc gcgcccgctt ccatctcctt gtcgcccacc accagcatgt
    11101 agggaatctt ctgcaactgc gcttcgcgaa tcttgcgatt catgcgctcg ctgctgtcgt
    11161 cgaccatgac gcgcgcgccg atctccttca gcgcggccgc catctgacga gcgtactcca
    11221 gatgccggtc ggcgataggg atgatcgcca cctgcacagg cgacaaccac accggaaacg
    11281 cgccgttgaa atgctcaatg aggatgccca cgaagcgctc catgctgccg aacggcgcac
    11341 ggtggatcat caccggccgg tgcttctggc cgtcctcgcc cgtgtactcc aggtcgaacc
    11401 gctccggcaa caggaaatcg acctgcaccg tgcccagttg ccattcccgc ttcaacacgt
    11461 cgcggaagat gaagtcgagc ttcggcccat agaaggcggc ctcgcccttt tcaaccgagt
    11521 agttgaggcc cagcttgtcg gccgcgttgc ggatggcggc gatgccgcgc tcccacatct
    11581 ccggctcccc ggcgtacttg tcgctcgtcg ggtcgttgat gcccagccgg gcgcggaagt
    11641 cactgaagcc aatcgtgctg aagacgtact ggatcaggtc gacgacggcg atgaactcct
    11701 cctcaagctg gtcgggcgtc acgaacaggt gcgagtcatc gaccgtgaag ccgcgcaccc
    11761 gcgtcaggcc cgtcagctcg ccgcttttct cgtagcggta gaccgtgccg aactcggcca
    11821 gccggtaggg caggtcacgg tagctgcgcg gctcgctgcg atagatctcg atgtggtggg
    11881 ggcagttcat cggcttcagc aggaactctt cctcatcgac cttgatcggc gtgtactggc
    11941 tgtccttgta ataagggtag tggccgctct tcttatagag ttccagattg ccgatgtggg
    12001 gcgtgatgac cggcagatag ccgcgctcca gttgcgcctg gcgcaggaag ctctccagcg
    12061 tctcgcgcag cacggccccc ttgggcagcc acagcggcag gccggagccg accagcggcg
    12121 agatgtggaa cagccccagt tgcttgccca ggcggcgatg atcgcgcaag cgagcctctt
    12181 cgaggcgctg caaatagtcg tccagctccg tcttgttgtg ccaggccgtg ccgtagatgc
    12241 gctggagctg ggggttcttc tcgtcgccac gccagtaggc gccgcccgtg cgcagcagct
    12301 tcacggcgtt ggacttgatg tcgcgcgtgc ggccgacgtg cggcccgcgg cagagatcga
    12361 cgaaatcgcg ctggcgatag atgcctactt ccgtcgccgg ttcggccgtc gggttgccca
    12421 tctcatcgac cttgcccgcg gccaggtcct tgattagttc gaccttgtag ggctgatcgc
    12481 ggaagaaggc cagcgcctcg tcgatgccca tcgtcgtgtg ctggaattcg gcgttgtcgc
    12541 gcagaagttc cttcatgcgc tcctcgatgc gctccaggtc ttcgggggag aacgtgcggg
    12601 gtttgccgcg ctcgtccttg cccaggtcga agtcgtagta gaagccgtcc tcgacgggcg
    12661 ggccgatggc cagcttgact tcggggaaca gatcgaccac cgcctcggcc atcacgtgcg
    12721 cggccgagtg ccggatgcga tagagttccg tctcgatata ggggatgttg ttgtctgaca
    12781 tgatacacct taattgaccg ctgactgctg accgccgacc gccgccggaa ttagctcggt
    12841 cggccgtcga cggtcggctg tcggttcaaa taaaaacgcc gccgccccgt attggggcga
    12901 cggcggtcgc ggttccaccc aatttcaaga gacgcgaacg tccctcaatc tcgattcggc
    12961 cgataacggg gcctggccgg gccgtcttat tgggtgggca gacgttctgt ctgccctggg
    13021 aggcacaacc ctgggtttgc ctcaaccgtt cggacggccg ctcccgggtg gttttcgtcg
    13081 ccttcgcttg cggtgagcac tcccagtcga tgatgcccac tctctggcgc gcgcttggcg
    13141 attactcgtc ccggtcaacg cgtttggcta tttgcttgtc gatgtgatgc attttttcaa
    13201 atacatcgta ctttattatg agtcttgatt atagatcaga gcagcgagat gtcaagaata
    13261 ttattcggat ggagtgcctt cccgaggggt tatgggtgtc gactcgataa cggttcgttg
    13321 ctcagcaatc cggcttacaa gttcctcgat ctctacaggt tcattatcag gacgacccag
    13381 gtagcgacca tcttcctgta aacgaaaata tgattcgaaa gggacaccca caaaagaaaa
    13441 cgtctcgcgc tttccctcgc gccacatagt gcttctttga ttggcaaaaa cgataacgat
    13501 gtcacctggt tgtaagtcat gtcccgcatc gctatggaga taagctcctc cttcgctcgt
    13561 ctgaactaaa accatttctt ccgccgggct attgccccat gttgtaatag taactttttg
    13621 gtcgacatcc atcgagccta caattggccg caatacttta aaagtgatcg tatggaacgg
    13681 tcgtcctcct tccaaatatt caccactttc ctgattgaaa tgagtcgtgg agatttcttc
    13741 caccaatccc tggaatacca tatccgcttg atcaatcatc tcatctcgtg tcagcgagat
    13801 aatttcactc gacacgatta tctgagtttg atcattctca gtttccccgg agatcggctc
    13861 tcttgaactg caagctatca gccctatgct actcagcatg aacatacagg aaagcgtaag
    13921 cgcttgtgtt gtctttgaca ttttcatcgg ttcgcctcaa cctgatagcg atcgattgac
    13981 cgtcaaacgt atcgccgaat gttggatgcg atagaggccc gtcgaaagaa atcacagcgt
    14041 catttcaaca tccccagcac cctacgaacg ccaccggtac ggttccggca cggggaaaac
    14101 accctcccac accacgttgc ggaaatagtc gggatcggta ttgccttcgg tgttgatcag
    14161 cagcacgcgg gagttttcat cgagttgaag ctgttgcctg agttcggccg cgccttcgta
    14221 gcccatgata aacggcagta cgcccagcgt gacggccccc gattcgccgg aaatcacaac
    14281 cgggtcgccc ttcaggggca cgccatacat gcgcatcccc ttggccgcca cataatcggg
    14341 ggcggtgacg atgaagtcgg cacaatcgcg gagtatttcc caggccaatg ggctgggttc
    14401 gccgcaggcc agcccggcca taatcgtatc cagatcgccg gaaaccgaat gcggccgacc
    14461 gtcgccgatc cggagggact gataaaggca atcagcctta cgcggctcaa cgacaaccga
    14521 aatgggcgcg ccggccccaa acagcttttc atagaaagca atcatggccg ccgcgaacgc
    14581 accaacgccc gcctgcagga atatatgggt cggcttcacg atcccttgcg cccctaactg
    14641 ttcctgggtc tcgctcagca acgtggtgta gccctgcatc acccattttg ggatatcttc
    14701 atagccgggc caggacgtgt cggagacgac ctgccagcca ttcgccagcg cgtctcgttc
    14761 acattggcgc acggcgtcgt catagttgcc atccaccacc cgcacttccg cgccgaactc
    14821 ggcgatggcg tcgattcgcg cctgggaggt gtgcttatgg acatagatga cgcatgggaa
    14881 gccaaacttg cgcgtcgccc aggccagccc cttgccatga ttgccgtccg tggcgctggt
    14941 gaaggtgatg tcgcccaagg tggcgcggat atcatcggac atcagttcct cgaatgacag
    15001 gctggggcga cccaccttcc ggcaaatgaa gcgataaatg gcaaaggagc cgcccagggc
    15061 cttgaaagca ttgagacgca tgcgcgccga ttcatctttc acccagatgt cgcccaaccc
    15121 cagtcgtgag gccaggttag atagacgctt gagaggggtc atctcaaagc gcggcatttg
    15181 catgtgaaac gcgcgggcct cttccgtcgt cccgtcgggc aacaagtggt cgaccgcctt
    15241 tctccctgcc tgggcatcca tatgcgggtt ttttagccag acaagtggcg tgtcatcatc
    15301 gaagttcatg atggattcct tttggtatat tttgtggacg gtatccctca actgccgctc
    15361 gccctgaatc gccgcgcgaa tgcggcggcg acacgcgcgg cctgcgcgcc ggcaaaggcg
    15421 tccatgcgtg tattgtagtc gggtggtggt gggttagcaa tgagtcgcgg cggcgcgggg
    15481 acgcaccatg ccggccgcgc gactcattcg tcaccgctgt gcttcttcca cagtgattga
    15541 atgtcgcagt gttccactac tcccccagcc ccaggggcct ggcggaggcg gataaaaaac
    15601 ctcgaagaaa gcaccgacaa acactgtgaa cgtttgaggt tcggccgggg ctgtgactgt
    15661 gaaagtggcg actccggttt tgccgggatc taccccctca ccctgatatt gagggagtgg
    15721 ctcccatatc ttcggttcag tgtggaggga atagaccggc ataccgaaat ggctgcaatc
    15781 atcgtttcgc aaccaggctg tgacctcgaa ttcctgtccg gcataaactg tcgccggcgc
    15841 ttcgagccac atcgtcgctt tgtacgtcag acactgcgcc gtcggcgcga ctgccacgat
    15901 cggcatatac gtccggtagg tcggtgggtc tgacggtaat gcgacaacac tcggcaagag
    15961 caagctcagc aaacttgcaa atatcgcttt ggccacgaga tgcattgttg gctcctccct
    16021 gagcgcgcct ccatgaatcg attaaaccgc ccgcggcatt gtctcaatca gcgcccgcaa
    16081 tacccgattc accgcgtctg aatcatggaa atattcggcc acgtccggtt caaggatcac
    16141 cacccgctcc tgatccttca gggcgaaccg gttgggtctg gccttccgat agtcgaattc
    16201 gtattccgcc ggcaaatcat cggattcggg gttattcgta ggtgttttct tcataatctc
    16261 tttatttccc gatccggcgc gtcggtctta agcatggtta ttccgacgac cgccagcgtc
    16321 ggggattggc attcccgtca acatagcgat tcataaaggc aaactcgacc gtgagcacga
    16381 acaaaatata gtgatcttgc ggcgtgtagg atgcggcgcg gacggcctcc tgccggatga
    16441 ggggatcgtc gctcaacgtc gcccgccccc tgacataaaa ctcaccgccg ccgccgctcg
    16501 aatcttccac cgaacagtgc agcgtatagc gagcatcccg ccccaggtcc ttgcccttgg
    16561 gcgaagtggg ttccataaac aggaacaact gctcgcccat aatgggcgtc accggatgca
    16621 cgcgcgggcc gccgtcggcg cgcactgtgc ccaggtaagc gacctcgctc tgaaatcggg
    16681 ttttgccgaa cgccgcaagc tcgggagccg cttgtgtgaa ttccgcccaa cttgccatgg
    16741 tttgacctcg cgccgctgaa tgggaagttt gaatccgcat ggactgcggc ctgtttacaa
    16801 gcgcgttcca ccgggaaagt atatcctttt tccaagggag aaccggcatg cacacgacca
    16861 attattcaaa ttgccggaat gagaggtcag aggtgtggta tactgaatcc atatggctgt
    16921 cctgggtata cggtggaccg tgcaagatcg agacggcaat tcggtttatt taaccgaaga
    16981 aaggtggcat catatcaccg aacccaacaa tcatcctgaa gtcgcagaat atgaagatgc
    17041 cttaaaaatg gcccttcgga ggggaaggcg gcgacaggaa ccgctcagtc cgcggaaata
    17101 tcgttacgct tatcctgttg aaggtttgcc tgatggcttt aatcatatcg tcgttatcgt
    17161 tctttttggt ttcaacgtag acgagcaagg tgcgacaatc ccgaataact ttatcgcaac
    17221 tgcttttctc aaacacattc gtcttaaagg tggtgttgaa tgatccctga agccacttat
    17281 acctacgact atgacaagga ggctgatgtc ctctatatct cattttcccc aagtgagagg
    17341 cctacggccg cggttgagct aaatgacaat atcctcttgc gtttcaatcg agccgaaaaa
    17401 cgcgctatcg ggctgacctt gatggacttc tcggttttgg tgcagttgac cgaattgggg
    17461 ccgcgcagtt ttccattgag tggtttggcg gacctggaac cggattggca ggaggcggta
    17521 attgaaatca tcaccgcatc gccagtgaac aggattctaa aggtttccag ttacatgcct
    17581 acggccgcgg aggtggtacc gattacgtcg gtggagcggc cgccgattcc attggcagtt
    17641 taagggtcat ccggacctgc aggaataaag cgggctgagt ctgctgcact aatcaagcca
    17701 gtatcttgat aattgccgag cttcgattat ggcagcccta acgctctccg acgcaggccc
    17761 tatcgaaggg gaaggtaaat aatgcgccct tcccattcat tttcaacgct gaactcggcc
    17821 aaaaggagaa tatcatcaat tacaatgcct atcgctgttg atttgctgac ttcgatcaca
    17881 ccgggcattg gttgattggc ttcgacacgc tggtaggcgt aatgggtgat cgtcgccacg
    17941 tcgtgggtaa gcagaaggcg ctgttgttcg gccgccaatt caaggattat ggagtcactt
    18001 gcaccggaaa ggccgacatc ctggacgcgg acaatgtcga tatccggccg gcgacggagc
    18061 aggccgcgaa cgatatcatt atcgaaattc tcgtcggcgg ccaggagcaa cacagtaact
    18121 cacaactctg tggcagcctg acgcgccaac aaacgctccc gcacgccggc aggatcggat
    18181 cgggtttcat tgagccggcg gacttgctca gcttccccac gtcgttgatg aagatagact
    18241 cctacttcat ccttgttccg caagtagtag cctataaccg agtagatgtc ggccagaggg
    18301 acagatgggt attgttgggc tatctcttcg gccgtcgcgc cgtcgttgaa agcctgcacg
    18361 atagtatcca gtgtgacgcg cgttttgctg acacggatga cgccgttgag gtccgtttcg
    18421 agtgggattg tctcgattgt gattagctca gtcattcgct ttatcctctg atactgatcc
    18481 attatacatt tggtcgtctt tggaagccac gcagaagcca caacatccac gaacatgaga
    18541 gcggcggcgg gcaaccacat agccggataa gtgtgatttg tgagcgcgcc gaatttcaag
    18601 tagcatcccc attcgcaaca aggagcaacg cttgaaaagt tacgcccacg ac
//
LOCUS       DAOMQG010000026        18381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1332_length_18381_cov_10.447452, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000026 DAOMQG010000000
VERSION     DAOMQG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 18381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18381
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_18381_cov_10.447452"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1597)
                     /locus_tag="PLC06_02990"
     CDS             complement(179..1597)
                     /locus_tag="PLC06_02990"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRO89442.1"
                     /translation="MTLVMKFGGTSVGSAAAMRETADLILQARDTWGQVAVVASAMGS
                     RPVKVTDLLLQGAQTAFTGDRETHQRLAVQLREIHYEAIDGLLSPEGERQIMLAEIER
                     FIERFTTLCGAVSVLGELTPRALDAISGMGEQMSVRILAAYLRELGYDSEAIDATELI
                     LTDGNFQNAAPLSAATREQTVARLQPLLDDDAIPVITGFIGATADGVTTTLGRGGSDY
                     SAALIGQALDADEVWIWTDVDGVMSADPRLVPNAVSIPTLSFREVSELAYYGAKVIHS
                     KTMRPCVESGIPLRIKNTFNPTHPGTVIEEDITQQNGALKAVTAIKGVAMITVEGKGM
                     IGVPGIAARTFGAVARLDVSVLLITQASSEQSICFIVPETAAAAVVRSLEDEFADELQ
                     RHDIERIWSRADVSIVTIVGAGLQTTYGIAGRVFSALGEHQVNVIAIAQGSTECGISV
                     VVAEGDIGRAIEHVHDLIVARG"
     gene            complement(1869..2114)
                     /locus_tag="PLC06_02995"
     CDS             complement(1869..2114)
                     /locus_tag="PLC06_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRO89443.1"
                     /translation="MSDKRLVRSRNDKMLFGVCSGLANYLNIDPVIVRLLAVLLTLWH
                     GVGLPIYLVLALIMPEEAEITPKANAFDEEEIVIKSS"
     gene            complement(2220..3395)
                     /locus_tag="PLC06_03000"
     CDS             complement(2220..3395)
                     /locus_tag="PLC06_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89444.1"
                     /translation="MDDFQADWMRRFSSLVAGALLFVILVAAGCSATSVVPTITPEPI
                     VPTRTPASKPIASATETPIPPSSPTPEASPIIPTLSAERAATLSRIGLAGALADAEPA
                     AALGLRSGHFTFWRVWDELPAVPGLTTWQTVRLGQVSEDMRWPANREVIARTLAAHPG
                     SYWLIGNEPDVIWQDNATPEEYARAYNEIYTFIKERDPSARVSAGGIALPTPLRLAWL
                     DEVLRIYRETYGHDLPADLWSIHLFILREERDSWGIDIPPGMDEAKGELYEIEDHGNV
                     ELVKQYVVAFRRWMAANSYADRPLAVTEFGILLPEDYGFPPEFVQNYMRQTYDYFLTA
                     TGEDGLAADDGRLVQYAFWYSLFDEGAYPTGNLYDARRGALTPLGEAFVRYIEELTP"
     gene            complement(3492..4904)
                     /locus_tag="PLC06_03005"
     CDS             complement(3492..4904)
                     /locus_tag="PLC06_03005"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRO89445.1"
                     /translation="MKANSVDELLRHLDGVVSVDRAIVTVHDELALREKIDELVYTAV
                     FSDGLVRDMARWLIWEIAQDLGIYPSSIHQLYMAIGSGDVPANFTVPAMNIRAMNYTS
                     SRAVFRAANKFNVGAMLFEIARSEIGYTGQRPAEYTTSVLAAAIKEGYRGPVFIQGDH
                     FQISLSRYKSMPDAEVQAVKDLMDEAVAAGFYNIDIDTSTLVDLGYPTLDEQQNLNYT
                     LCAQLTEYVREIEPEGISVSLGGEIGEVGHKNSTVEELHAFMQGYQRVLAKGLPGISK
                     ISVQTGTSHGGVVLPDGTLAQVKVDFDTLRDLSRVAREDYGMGGAVQHGASTLPPSAF
                     NKFPQIGTVEIHLATNFQNIVFDYLPKEVVDEAYAYLREHHKDEWKPGKTDEQSLYSA
                     RKRAIGPFKAQWWNLDESRLAEIGGVLQEQFEFLFDQLNVKGTRELVEQVTTRVVIHQ
                     PKPTAAAEAVAAEDVKDLAD"
     gene            complement(5095..6180)
                     /locus_tag="PLC06_03010"
     CDS             complement(5095..6180)
                     /locus_tag="PLC06_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRO89446.1"
                     /translation="MAEPKQVIQSIDQVTPEWLTYVLTRSGALTGGRVAAIAIESGER
                     ILSTSTRLRIAYSEGATGALPPKLFLKTVNVDQDEDFFGASEVNYYLRDYKGVACAPI
                     PRCYDAVFSEEQRCYHLLLDDLADTHIEAKHKPRTLEYGLALAEGLACLHAAWWGGDR
                     LADVGEQIPVARQIDRFVEIAQPGAGHIIGAYSRELKAYWPAAVEEILQRHPSVMIAR
                     TNDSNGFTLIHGDPNWNNILVPHNADRPLYIIDRQPFDWSLTVWLGVYDLAYALVLDW
                     DIDERRRFEMPILRHYHDRLMTHGVRDYTWEQLLLDYRLSVPICVYVAIEWCRGGVNE
                     PWVHIWLPMLQQAMTACDDLNCRELWG"
     gene            complement(6236..6763)
                     /locus_tag="PLC06_03015"
     CDS             complement(6236..6763)
                     /locus_tag="PLC06_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89447.1"
                     /translation="MKILETDRLLLRRLVPGDLDALYALYRDPEMRQYFPPEGVSPDK
                     TLTYEETKEELEWFLNGHPRHPELGLWATILKENGEFIGRCGLLPWTIDGQDEVEVAY
                     MIDKRYWGLGLGTEAARGIRDYAFAQLDLSRLVCMILHENMASVKVATNIGMGFEKEG
                     IDELGPFLVYSMSRG"
     gene            complement(6760..7242)
                     /locus_tag="PLC06_03020"
     CDS             complement(6760..7242)
                     /locus_tag="PLC06_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89448.1"
                     /translation="MEIRPVEHSDATEWLRMRAALFTDARPEEEANEIGCFFSGALPP
                     TLHAAFVCPRPDGGLCGFVEVSIRPYADGCETDNVGYLEAWYVDADARGRGVGRALVM
                     AAEEWACAQGCREMASDTDLTNFDSQASHARLGFEEVGRIVQYCKVIHPPKADAYKKL
                     "
     gene            7613..>9729
                     /gene="alaS"
                     /locus_tag="PLC06_03025"
     CDS             7613..>9729
                     /gene="alaS"
                     /locus_tag="PLC06_03025"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRO89449.1"
                     /translation="MRPTTSNEIRKAFLDYFNELKHEVVPSAPLPQRDNPTLLFTNAG
                     MNQFVDVFLGKEKRPYSRATTAQKVMRVQGKQNDLENVGPSARHHTFFEMLGNFSFGD
                     YFKREAIDYAWTFLTGVMELEPERLFATVYTDDDEAAKLWERYLPVERILRFGKKDNF
                     WAMGDVGPCGPCSEIHYYSGDMKDINPVGVNNDDFPNYVEVWNLVFMQFDAQPDGSLV
                     PLPKPSVDTGMGMERLVRVIQGVDSNYDTDLFVPVLARVQALLGDSDEQREKNYVGYR
                     VVADHGRAATFLIADGVQPGNTGAGYVLRMIIRRAARFGRKIGFHEPFLGEVAQVYID
                     QMGEAYPELRQRRDLILYTLRREEERFNRTLDNALLHLDTLIDELHSQGITEIPGDVA
                     FNLYATYGLPLEITRDVAREQHDMTVDEDGYNAAREAHALASGSGAFTSYVVGANVYT
                     DLLRALIENGYLEGSGVDYDPYSGSSLETTVVGLIRDGERVQSAYAGDKIEVVTAATP
                     FYVEAGGEVSDTGRITATGATLIAIRDTRRPTPGLIVHIGEVVSGEINEGDLVRLEVD
                     RARRADIRRNHTATHLLHRELREHLGKHVVQAGSLVAPDRLRFDFTHDKAVDRETLGQ
                     IEAAINDAILSNYPVKIAYMGQKEAIAAGAMALFGEKYGDIVRTVTVSESASNGNATP
                     PYSFELCGGLHVGETGEIGQFRF"
     assembly_gap    9730..9829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9830..10186
                     /locus_tag="PLC06_03030"
     CDS             <9830..10186
                     /locus_tag="PLC06_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRO89450.1"
                     /translation="ILLGGLVQVSDAYVLAARVDVQNVDQLREMADWFRDRVKSGVAV
                     LGAINNGKPIIVATVTDDLIARGVKAGDLVREVARVVGGSGGGRPNMAQAGGRDPEKL
                     GEALAIVKGMVETALS"
     gene            10260..11459
                     /locus_tag="PLC06_03035"
     CDS             10260..11459
                     /locus_tag="PLC06_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89451.1"
                     /translation="MGIQIIELDPADTFFSARDRLLRSGRGRAVLLFPGRSRLLSGID
                     LVLLRRLADRERLDIGLVTTNRELSRQARALGLPVFANLTLAEHYRPGWWRARRRSEI
                     LGFAQGDIRSPWAPNAGLADLHPKSPARRLAHFIALLIIPLVVALAIIAAIYLLPRAT
                     LVLEPEMQPAQVILTLTTGAQATELTDSTVPTRAVRHELEWAASGPTTTDAEADLQRI
                     RAQALQGLGAAAPDYLAPRIAPGELLVPASARVEILEESLERDETEARLTMRVALTGA
                     AVAAADVNRIAYRRLAETLPDGVEPDPATVTIQLDPAAGEANSFQVTAQAIGQPVFDQ
                     EAIAESLSGMRAGDAAHYLDMTLRLAEPPTFDVWPSWWWSWSRGRLPLRADRIRVEIS
                     PSRATVD"
     gene            11542..11994
                     /gene="ruvX"
                     /locus_tag="PLC06_03040"
     CDS             11542..11994
                     /gene="ruvX"
                     /locus_tag="PLC06_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRO89452.1"
                     /translation="MTHSTMPGRVMALDLGEKRIGIAISDPMRIIASPHSVLNRKSRA
                     DDFAHYARLIAEQEVTLLVIGLPITLGGEEGQRAAWVRDYCADLGRHIDIPIAFHDES
                     LTTVAATAALHAQGRRGRKAKERVDAVAAALILQSYLDAQPGEKRDGE"
     gene            11984..13168
                     /gene="mltG"
                     /locus_tag="PLC06_03045"
     CDS             11984..13168
                     /gene="mltG"
                     /locus_tag="PLC06_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRO89453.1"
                     /translation="MANKPARNGLSPFGYLWRILVVGTLLIVTAVASWMFYRTWLGEG
                     LDRPAANPNLSQSRRLYLEYYLTERAAEMQRPAGTATGPQALTVSPGEGAAAIAQNLQ
                     AVGLLTNTELFLNYLTYYGLDSGLVAGQYTIEPGLTIPQLVEALGAGGARALELSFLP
                     GWRSEEMANYLRVVTPGQIDGDAFLDIVHNPHRNDLSNFPFFSSLPDGATLEGYLFPG
                     AYSIEPATDSAALVRMMLERFDQQVTPAMRQAYGAQGLSLREALILASIIEKEAMLAE
                     EKPIMAAVFLNRLRSGMPLQADPTVQYALGYHAETDTWWKSPLDAADLDIASPYNTYQ
                     INGLPPGPISNPGLSSLEAIAAPADADFLFFVLDCAAATTGRHIFSATYEEHLANVER
                     CR"
     gene            13149..13775
                     /locus_tag="PLC06_03050"
     CDS             13149..13775
                     /locus_tag="PLC06_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89454.1"
                     /translation="MSNVAARALLVLLATLLVTGCASADPVVKIGLVAPFEGRDRAIG
                     YDAIYSARLAVRQINEAGGVAGHRVALVALDDRGDPELAKQAAASLAVDPGVVVVLGH
                     FLPEITTIAAPVYEQHQLALLPMGETPFAPVSPNSLPPEFLGAYEAITPFDEIAGPFA
                     GPTFDAMGLVRLALAEAEESTGSISRASVQEALDGLKYQGLTGEVYLP"
     gene            13810..14820
                     /locus_tag="PLC06_03055"
     CDS             13810..14820
                     /locus_tag="PLC06_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89455.1"
                     /translation="MIIRRILGLIMLLTGLTLLAASLAGAYFVGGVLNRFADSVGRTV
                     DVASTSLDTARGTLELARDSLGDVDGGLATAVTATAGAARTLDESRPLLDNVAIVTTQ
                     EIPEAIEGMQTALPNLIEVAGVIDRTLTTLSSVGIDQTIPLPFGGSIPLKFDLGIDYN
                     PTTPFDESLRVFEASLVGLPESLRGLEDDLATTNANLASLSADLQATSDNLATINTRM
                     GDLSDLFDQYVLLVDNVTGLLAQLETQLAPRLELIRIVAIALLIALGLSQLAPIYLGW
                     ELLTGRRDPQAHINNVAVVSDVPPAFATSDIPTSNYSADLHPDSAQSPGAATGEETHA
                     QT"
     gene            14807..17410
                     /locus_tag="PLC06_03060"
     CDS             14807..17410
                     /locus_tag="PLC06_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRO89456.1"
                     /translation="MPKPKSKAVTLSLALFALIAALLTAGCRGQASTEPTAQVPSDVP
                     GLLPTPTLAAPGSVDPLRPPTPIVMPSPTPLSSDVIETPSEMEQPNLEAPLEVQPTPT
                     VTLPEGATPAELLVLGQTAAAEGDHLMSAAAFRAVVDRPGDLATGEIATARLGLAVAL
                     MALGDHSAAAVELEAVVQEPQFTADPGKGGALLATVNTADVAAFHLGQVRARMGDHAG
                     AIDAYRAYLQNNPDMAAYVEPLIANAYEALGDTEGVITALEGAVTGPAQRFKAVANRM
                     RLAELYLARQEYANAIAQYDAVHDIARTEATKGQMTYLAGRAEIMAGNTAAGHERFQR
                     AITDYPRAAESYQALVALVDAGIPVDEYQRGVVDYYAGAYLPAVEAFRRYLSGAGDEY
                     NTDAHVFLAWSLERLGDVENALAELDTLTKRDPARALFERAGILHRAGRQDEALAAYD
                     EFLESHGDTEGAAGVAWSAAALADEMARDDTSARYLFLADTFPFDENAPLALFRAADL
                     LQAGGDDAEATALRLRLVEQYPANIFGAEALFRLLQAAESDPTGEPTRAELMEQVASL
                     SPTNYFALRAADLAAGIAPFTAAGPMNLAIDPDEGRAEAEAWLRERLIADGVEVPAGE
                     LGDLSLDLAADPHRIIGEKLWQLGLQEEAKAELELVRESYAGDPLATYRLARFFGELG
                     LYRSSIIAAAGLLRDLGATVFDAPLYLGRLSYPVYYSDLIMPLAERYDFDPRIQFALV
                     RQESLFESFARSGAAAQGLSQVIPDTGAWIAQRLAWPNFVNEDLYKPYVGLNFGAYYL
                     SQQLHNFDGHVHAALAAYNGGPGNAARWLEVAGVDLDRFVDAVDFPETRLYIERIYEG
                     YNAYRHLYGGP"
     gene            17579..>18381
                     /locus_tag="PLC06_03065"
     CDS             17579..>18381
                     /locus_tag="PLC06_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89457.1"
                     /translation="MPTNFDWQTEEDNRRRPESLWDDSSGSPAGPPARRRPWRMIGLI
                     ATLVLIVGSVVWWRVDKYIDATLQANRNDVIASHNLLQRAVADSDEEVFRSVLSGRMP
                     AWTDAELDVFYRQLVFDRGPLGLTPVEGSLPVILSTTEGEVDADQRTADIELSPDMNE
                     AVVVVDHPFQVTATGETVVLRQTSIFRRGGSRWLLSPPLDEFWGDWITVEGEYLSLIY
                     PDRDAPTAARLAADLDEEIGRMCATLEEINCSGDLHLTVRLDTDPTSLAA"
BASE COUNT         3663 a         5841 c         5254 g         3523 t
ORIGIN      
        1 ccgatgccgc tggacgcgcc ggtgatcaat gcccggccga caggtatcag ttcccgatcg
       61 gccgttgccc gccgaagcgc gcgccaagcg gccgtccccg cgatagctgc gcccagcccg
      121 gctgccacaa ctgccccgcc cgaccaaccc ccgctctttt tctgtttttc cattcaactc
      181 accccctggc cacgatcaaa tcgtggacat gttcaatcgc ccggccaata tcaccctcag
      241 cgacaaccac actaatgccg cattcggtcg acccctgagc aattgcgatg acgttcacct
      301 gatgctcgcc cagcgcgcta aatacacgcc cggcaatacc ataggtcgtc tgaagtcccg
      361 ccccaacaat agtcacaatg gagacgtccg cgcgggacca gattcgctcg atgtcgtgcc
      421 gctggagttc gtcggcaaac tcatcctcca ggctgcgaac aacggccgcg gcggccgtct
      481 cgggcacaat aaagcagatt gattgttccg acgacgcctg agtgatcagc aaaacactca
      541 cgtcgagccg cgccactgcg ccaaacgtcc gcgcagcgat acccggcaca ccgatcatcc
      601 ctttgccttc aacggtgatc atggcaacac ccttgatagc cgtaacagcc ttcagcgcgc
      661 cgttctgttg ggtaatatct tcttcgatga ccgtgcccgg atgggtgggg ttgaaggtgt
      721 tcttgatgcg cagcgggatg ccgctctcca cacaggggcg catcgtcttg gagtgaatca
      781 ccttggcccc ataataggcc agctccgata cctcgcggaa cgacagcgtc gggatcgaga
      841 cggcattggg taccagacgc ggatcggccg acattacgcc gtcaacgtcg gtccagatcc
      901 acacttcatc ggcgtccagc gcctgaccaa tgagggcggc gctgtagtcg gagccgccgc
      961 ggccgagagt ggtggtcacg ccgtcggccg tcgcgccgat aaaccccgtg atgacgggga
     1021 tagcgtcgtc gtcgagcagc ggttgcaggc gggccactgt ctgctcgcgg gtggcggccg
     1081 aaagaggcgc ggcgttctgg aaattgccgt cggtcagaat cagctccgtg gcgtcgatag
     1141 cctccgagtc ataccccaat tcgcgcagat aagcggccag gatgcgaacg ctcatctgct
     1201 cgcccatgcc gctgatggca tccagcgcgc gcggggtcaa ttcgcccagc acgctgaccg
     1261 cgccacacag ggtagtgaat cgttcaatga accgctcgat ctcggccagc ataatctggc
     1321 gttccccttc cggggacagc aggccgtcga tcgcctcata gtggatctcc cgcaactgaa
     1381 cagccagccg ctgatgggtc tccctgtcac ctgtgaaggc cgtttgcgcg ccttgcagca
     1441 acaagtccgt caccttcacc ggcctggaac ccatggccga ggcgacgaca gcgacctgtc
     1501 cccaggtgtc gcgcgcttgc aggatcagat cggccgtctc gcgcatagcc gccgcgctgc
     1561 cgaccgatgt cccaccaaat ttcatcacta acgtcattgt ttctatcctc agccaggcaa
     1621 gtagcctagc gatccatgag tatagccaag cggaaacggc gggcaatggg aaaagcggcg
     1681 tcaaaacagg aaacagccga tcccgtaacg agatcggctg tttcatgctt catccggcgg
     1741 caggagattg gaaacggata atctttcgcc ggaaaaaagc caagtaaatc acccggcacg
     1801 atcaacggcc tgccaatacg gaagataccg ccgtcgagcc gcgccgggcg agccggtaag
     1861 cagcgagttt atgaactctt gatgacgatc tcttcctcgt caaaggcgtt ggccttcggc
     1921 gtgatctccg cctcttccgg cataatcagc gccagaacga ggtagatcgg caaacccacg
     1981 ccgtgccaaa gggtaagcag gacggccagc agccgaacga tcaccgggtc aatattcagg
     2041 tagttggcca ggccactgca tacgccaaac aacatcttgt cattgcgaga acgaaccaat
     2101 cgcttatcac tcatttcgaa cctccagtgt tcatatcgat gttctgtgac tctatacgcc
     2161 acctcgattc gaaagttgca ggggttttta cgaccggaat aaccggctgc gggcaaccgc
     2221 tatggggtca gctcttcaat ataacgaacg aatgcctcgc ccaggggcgt aagcgcgccg
     2281 cgccgggcgt cgtaaaggtt accggtcgga taagctcctt cgtcaaagag gctgtaccaa
     2341 aaggcatact gcaccagccg tccgtcgtcg gccgccaatc catcttcccc ggtcgccgtc
     2401 aggaaataat cgtatgtctg ccgcatgtag ttctgaacga attcaggcgg gaagccgtaa
     2461 tcctccggca agaggatgcc gaattctgtc accgccagcg gcctatccgc atagctgtta
     2521 gccgccatcc atcggcgaaa cgccaccaca tattgcttca cgagttcgac attcccgtga
     2581 tcctcgatct catacagctc ccccttggcc tcatccatgc ccggcgggat gtcgatcccc
     2641 cagctatccc gctcctcgcg caagatgaaa agatggatcg accacagatc ggccggcaga
     2701 tcatgcccat atgtctcgcg gtaaatgcgc aatacctcat ccaaccaggc caatcgcaag
     2761 ggcgtcggca aggcaatgcc acccgcactc acacgggctg aggggtcgcg ctccttgatg
     2821 aaggtgtaga tctcgttata ggcgcgggcg tattcctcgg gagtagcgtt gtcctgccag
     2881 atgacgtccg gctcgttacc gatgagccag taggagccgg ggtgagcagc cagcgtcctg
     2941 gctatcacct cacgattggc cggccagcgc atgtcttccg acacctgccc cagccgcacg
     3001 gtctgccatg tcgttagccc cggcaccgcc ggcagttcat cccagacacg ccagaaagtg
     3061 aaatgtccgg acctcagccc cagcgcggcc gccggttcgg catcggccag cgctcccgcc
     3121 aggccgatac ggctgagcgt cgcagcccgc tcggccgaca aggtgggaat aataggcgat
     3181 gcttcgggtg taggcgatga aggaggtatc ggtgtttccg tggcggaagc aatcggtttc
     3241 gaggcaggcg tgcgcgtcgg cacgatcggc tccggcgtta tggtgggcac gacgcttgtc
     3301 gccgaacaac cggccgcgac caatatcaca aacagcagcg cgcctgcaac cagtgacgaa
     3361 aaacgccgca tccaatcagc ctgaaaatcg tccatacact cctgataaca gaaagagcgg
     3421 cgctcgtaca aagccgccgc tcttctccat gacttgattg atcccgcaaa ggcgggagtc
     3481 acccgtcagc gtcagtcggc gagatccttg acatcctcag cggccacggc ttcggccgcc
     3541 gcagtgggct tcggctgatg gatgacgacg cgggtcgtca cctgttccac cagttcgcgc
     3601 gtccccttga cgttcaactg gtcgaacagg aactcgaact gctcctgcag aacgccgccg
     3661 atctcggcca gtcgggattc atccagattc caccactgcg ccttgaacgg gccaatggcg
     3721 cgcttgcggg ccgaatagag gctttgctcg tcggtcttgc ccggcttcca ttcgtccttg
     3781 tgatgttcgc ggaggtaggc atacgcctca tcgaccactt ccttcggcag gtaatcaaag
     3841 acgatattct ggaagttggt cgccaggtga atctccaccg tcccaatctg cgggaacttg
     3901 ttgaaggcgc tcggcggcag ggttgacgcg ccatgctgca ctgcgccgcc catgccgtaa
     3961 tcctcgcggg ccacgcgact caggtcgcgc aatgtgtcga agtccacctt cacctgggcc
     4021 agcgtgccgt cgggcagcac gacgccgccg tggctcgtgc cggtctgcac cgagatcttg
     4081 gagatacccg gcagcccctt ggccaacacg cgctgataac cctgcatgaa ggcatggagt
     4141 tcctcaacgg ttgagttctt gtggccaact tcgccgattt cgccacccag tgagacactg
     4201 atgccctccg gctcaatctc gcgcacatac tccgtcagtt gggcacaaag ggtgtagttc
     4261 agattttgct gctcgtccaa cgtcgggtag ccaagatcga ccagagttga ggtatcgata
     4321 tcgatgttat aaaaaccggc ggcgacagcc tcatccatga ggtccttcac ggcctgcact
     4381 tctgcgtcgg gcatgctctt gtagcggctg agcgaaatct ggaaatggtc gccctgaatg
     4441 aagaccgggc ctcgatatcc ttccttgatg gccgccgcca gaacgctggt ggtgtattct
     4501 gccggacgct ggccggtgta gccaatctcg ctgcgggcga tctcgaacag catcgctccg
     4561 acgttaaact tgttggcggc acggaagacc gctcgcgacg aggtgtagtt catggcgcgg
     4621 atattcatgg ccgggacggt gaaattggcc ggcacatcgc cgctgccgat ggccatatac
     4681 aactgatgga tcgaggatgg gtagatgccc aggtcctgag cgatctccca gatcagccag
     4741 cgggccatat cgcgaaccag accatcgcta aaaacggccg tataaaccag ctcgtcgatt
     4801 ttctcgcgca gagccaactc atcatgcacc gtcacgatcg cacgatcgac ggatacgacg
     4861 ccatccagat ggcgcagcag ttcatcaacg gaatttgcct tcatgctcaa atctcctctt
     4921 atggatctat ctgtcaaatt gcgggtatgt tcgcgctttt tggtcggaaa tacggcttgc
     4981 gcagccggca ggcggcgggc cggatgattg aacaaacgat caatccaatc aggatcgtat
     5041 catacttcaa acggaaggat cacgacaatt agcacctctt taaggtgatc aatgtcatcc
     5101 ccacaactca cggcaattca gatcgtcaca agcagtcatt gcctgttgca acatgggcag
     5161 ccatatgtgc acccatggct cgttaacccc accgcgacac cactcaattg ccacataaac
     5221 gcatatcggc acagagaggc gatagtctag aagaagttgc tcccaggtgt aatcgcgaac
     5281 gccatgggtc ataagccgat catggtagtg gcgcaatatc ggcatctcga agcggcggcg
     5341 ctcatcgatg tcccaatcga gcaccagcgc atatgccaga tcgtagacgc ccagccacac
     5401 agtcaggctc caatcaaacg gctgacggtc tatgatgtag agcggccgat cggcattgtg
     5461 cgggacgagg atattgttcc agttgggatc gccgtggatc aaggtgaagc cgttggaatc
     5521 gtttgtccgc gcaatcatca ctgagggatg gcgctgcagg atttcttcaa cggccgcggg
     5581 ccaataggct ttcagctcgc gggaataagc gccgatgatg tgaccggctc ccggttgggc
     5641 aatttcgaca aaccgatcga tctgccgagc aacagggatt tgctcgccca catccgccaa
     5701 acgatcccca ccccaccagg cggcatgaag gcacgccaac ccttcggcca gcgccaagcc
     5761 atactccagt gtgcgcggct tatgcttggc ctcaatatgg gtgtcggcca gatcgtcgag
     5821 caacaaatga tagcagcgct gttcttccga gaacacggca tcataacacc gaggaatcgg
     5881 tgcacaggcg acgcccttgt aatcgcgcaa atagtagttg acttcagatg cgccaaagaa
     5941 atcttcatcc tgatcgacat tgacggtctt caggaacagc ttgggcggca aagcgccggt
     6001 ggcgccctca gaataggcta tacgaaggcg agtgctggtc gacaggattc gttcgccgga
     6061 ttcgatggct attgctgcca ctcggccgcc ggtcaacgcc ccactcctcg tcaatacata
     6121 ggtcaaccat tccggcgtca cctgatcgat tgactggatt acctgcttcg gttcggccat
     6181 cttcttttgt catcgaatgc tattttgcga ccgcaccctg tccgccatcg gccaactacc
     6241 cacgactcat cgaatagacc aaaaaaggac caagctcatc gataccctcc ttctcaaatc
     6301 ccatgccgat gtttgtagca actttaaccg acgccatatt ttcgtggagg atcatgcaga
     6361 cgagacgtga aagatctaac tgagcgaagg cgtaatcgcg aatgccgcgg gctgcctcgg
     6421 tgcccagacc caatccccaa tatcgcttgt cgatcatgta agcgacttcg acttcatcct
     6481 gcccatcaat cgtccagggc agcaacccgc agcggccgat aaactcgccg ttctccttca
     6541 gaattgttgc ccacaggccc agctcaggat gccggggatg accattaaga aaccattcca
     6601 gttcctcctt tgtctcctcg taagtcagcg ttttatcggg ggatactcct tcgggcggga
     6661 aatactgtcg catttccggg tcgcggtaga gggcatagag tgcatcgaga tcaccgggaa
     6721 cgagacggcg gagcagaaga cggtcggttt ccaggatttt catagctttt tatatgcgtc
     6781 agctttcggt ggatggatca ctttgcaata ctgaacaatt cggccaactt cctcaaagcc
     6841 caggcgtgca tgagaagctt ggctatcgaa attggtcaga tcagtatctg aggccatttc
     6901 ccgacaacct tgtgcgcagg cccactcttc ggcggccatg accagcgcgc gcccgactcc
     6961 gcggccgcgc gcatcagcat caacatacca cgcctccaga tagccgacat tatcagtctc
     7021 gcatccgtcg gcataggggc gaatactcac ctcgacgaac ccgcacaacc cgccatcagg
     7081 gcgcggacag acaaaggcgg cgtgcaaagt tggaggcagc gcaccggaga aaaagcaccc
     7141 gatctcattc gcctcctctt cgggcctggc atccgtaaac aaggccgcgc gcattcgcag
     7201 ccattctgtg gcgtctgaat gttcgacggg gcgaatttcc ataatatacc ctcgcaggat
     7261 cacgatattg tatcatacct gccctaacca tcagagattt gcagattgat cggccgcgag
     7321 atttgaacct gcccaagaca taattgcatc aatggagtgt agagattata accccagtcg
     7381 atgattgact attcccctat ctgcggttat atttctctca tgttgaattc gccgtccttg
     7441 cactcgcaat tcgccgccgc cggccggaca tccagtctgg tcggagacgt tgcttgacgc
     7501 gcgcacaaca acttacgcga aacggcaacc gccagactga acgctcagtc tggcggtttt
     7561 tatttccgtc ggcggtcaat ttgaaatctg ctaaaattca tccggagaaa ttatgagacc
     7621 gacaaccagc aacgagatac gaaaagcctt tcttgactac ttcaacgaat tgaaacacga
     7681 agtcgtcccc agcgccccgc tgccgcagcg cgacaacccg acgctgctgt tcaccaacgc
     7741 cggaatgaac cagttcgtcg atgtcttcct gggtaaagag aagcgcccct atagccgcgc
     7801 caccaccgcc cagaaagtca tgcgcgtgca gggcaagcag aacgacctgg agaacgtcgg
     7861 cccctccgcc cgccaccata cgttcttcga gatgctcgga aacttctcct tcggcgatta
     7921 cttcaagcgc gaggccatcg actacgcctg gaccttcctg accggcgtga tggaactgga
     7981 gccggagcgc ctcttcgcca ccgtctacac cgacgacgac gaagcggcca aactctggga
     8041 acgctacctg cccgtcgagc gcatcctgcg cttcggcaaa aaggacaact tctgggccat
     8101 gggcgacgtc ggtccctgcg gaccatgttc cgagatccat tactactccg gcgacatgaa
     8161 ggacatcaac ccggtgggcg tcaataacga cgatttcccc aattacgtcg aggtgtggaa
     8221 tctggtgttc atgcagttcg acgcccagcc ggacggttcg ctggttcccc ttcccaagcc
     8281 atcggtcgac accggcatgg gcatggagcg actggtgcgc gtcattcagg gcgtggatag
     8341 caactacgac acagacctct tcgtcccggt cctggctcgc gtccaggcat tgctgggcga
     8401 cagtgacgag cagcgcgaga agaactatgt cggctatcgg gtcgtcgccg accacggccg
     8461 cgcggccacg ttcctcatcg ccgacggcgt tcagccgggc aacaccggcg cgggctatgt
     8521 cctgcgcatg atcattcgcc gggcggcgcg cttcggccgc aagattggct tccacgaacc
     8581 gtttctgggc gaggtggccc aggtctacat cgaccagatg ggcgaagctt atccggagtt
     8641 gcgccagcgc cgcgatctca ttctctacac cctgcgacgc gaggaagagc gcttcaatcg
     8701 cacgctcgac aacgcgctgc tccatcttga tacgctgatt gatgagcttc acagtcaagg
     8761 tatcacagag attcccggcg acgtagcctt caacctctac gcgacctatg gcctgccgct
     8821 ggaaatcacc cgcgacgtgg cccgcgaaca gcacgacatg actgtcgatg aggacgggta
     8881 taacgccgcg cgcgaagctc acgctctggc cagcggcagc ggcgcattca ccagctatgt
     8941 ggtcggagcc aatgtctata ccgatctgct gcgcgcgctc atcgaaaacg gctatctgga
     9001 agggtccggc gtcgattatg acccctattc cggctcatcg ctggaaacca ccgtcgtcgg
     9061 cctcatccgc gacggcgagc gagtacagtc ggcgtatgcc ggagacaaga tcgaagtcgt
     9121 cactgccgcc acgcctttct atgtcgaggc aggcggcgag gtcagcgaca cgggccgcat
     9181 caccgccacc ggcgcaacgc tcatcgccat tcgcgacaca cggcggccga cgccgggact
     9241 gatcgtccac atcggcgaag tcgtctccgg cgagataaac gagggcgacc tcgtgcgcct
     9301 tgaggtcgac cgggcgcggc gggccgacat tcgccgcaac cacacggcca cccacctgct
     9361 ccatcgcgag ttgcgcgagc atctgggcaa gcacgtggtt caggccgggt cgctggtggc
     9421 tcccgatcgc ctgcggtttg acttcaccca cgacaaggcg gtggatcgcg agacgctcgg
     9481 ccaaatcgaa gcggccatca acgacgctat tttgtccaac tatccggtca aaatcgccta
     9541 catgggccag aaagaggcga tcgccgccgg cgcgatggct ctgttcggcg aaaaatacgg
     9601 tgacatcgtc cgcaccgtta ccgtgagcga aagtgcatcc aacggcaacg cgacgccacc
     9661 ctacagcttc gagctatgcg gcggcctcca tgtcggcgag accggcgaga tcggccaatt
     9721 ccggtttatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttctgctcgg
     9841 cggtctggtc caggtcagtg acgcctatgt gttggcggcg cgagtggacg tgcaaaacgt
     9901 tgaccaactg cgcgagatgg cggactggtt tcgcgatcgg gtcaaatcgg gtgtcgccgt
     9961 cctcggcgcg atcaataacg gcaagccgat catcgtcgct accgtgaccg acgacctcat
    10021 cgcacgcggc gtgaaggccg gcgatctggt gcgcgaggtg gctcgggtcg tcggcggcag
    10081 cggtggcggt cggccgaaca tggcccaggc cggcggccgc gacccggaga agctgggcga
    10141 agcgctggcc atcgtcaagg gcatggtcga gacggctctt tcctgacggc catagaataa
    10201 cgggctagat ttcacaaacg acgggtcgcg gatcacatct ccgcgatctt cggctcctta
    10261 tgggcataca aatcatcgaa ctggacccgg ctgatacgtt cttctcggct cgcgaccgcc
    10321 tgctgcggag cggccgcggg cgcgcggtcc tgctgtttcc tgggcgctcc aggctgttga
    10381 gcgggatcga tctcgtcttg ctgcgccgcc tggctgaccg tgaacggctg gacattggtt
    10441 tagtgacgac caatcgagaa ttatcccgac aggcgcgggc attgggtctg ccggtctttg
    10501 ccaatctaac gttggccgag cattaccggc ccggctggtg gcgcgctcga cgacgctccg
    10561 aaatactggg ctttgcgcag ggggatatcc gtagcccatg ggcgccaaac gcagggttgg
    10621 ccgatcttca tccgaaatct cccgctcgtc gtctcgcgca tttcatcgct cttttgatta
    10681 tcccgctcgt ggtggcactg gcgattatcg ccgcaatcta cctgctcccg cgcgcgacgc
    10741 tcgtccttga gccggaaatg caaccggcgc aagtgatcct cacgctgacg accggggcgc
    10801 aggcgactga acttacggac tccaccgtcc cgacgcgcgc cgttcggcac gagcttgaat
    10861 gggcggcctc cggcccgaca accaccgatg ccgaggccga tctacagcgc atccgtgccc
    10921 aggcgctgca agggctgggc gcggccgcgc cggactatct cgcgccccgc atcgctccgg
    10981 gcgagctact cgtccccgcc tcggcgcggg tcgaaatatt ggaagagagc ctggagcgcg
    11041 acgaaaccga agcgagattg acgatgcgcg ttgcattgac cggcgcagct gtggccgccg
    11101 ccgacgtcaa ccggatcgcc tatcgacgat tggccgagac tctccccgat ggggttgagc
    11161 cggatccagc gaccgtgacg attcaattag acccggcggc cggcgaggcg aactctttcc
    11221 aggttaccgc ccaggccatc gggcaaccgg tgttcgatca ggaagccata gccgaaagct
    11281 taagcggcat gcgcgccggc gatgccgccc attatctgga tatgaccttg cgattggccg
    11341 aaccgcccac gtttgatgtt tggccgagct ggtggtggtc ctggtcccgc ggccgcttgc
    11401 ccttgcgcgc cgatcggatt cgggtggaaa tcagcccatc aagagcgacg gtggattaat
    11461 ggcggaacgt agggaaacca ttcgggccgg ccggtcgctc aggtggataa ttgacgtgga
    11521 acaagcaccg gaactacgat gatgactcat tccacaatgc ccggccgcgt catggcgctg
    11581 gacctgggcg agaagcgcat cgggatcgcc atcagcgatc caatgcgcat catcgcctcg
    11641 ccccactccg tgctgaaccg gaaatcgcgc gctgatgatt ttgcccatta cgcccgcctc
    11701 atcgccgagc aagaggtcac tctcctcgtg atcggcttgc ccatcacgct gggcggcgaa
    11761 gaggggcaac gcgccgcctg ggtgcgcgat tactgcgccg atctcggccg ccatatcgat
    11821 atccccattg cattccatga tgaaagcctg acaaccgttg ccgccacggc cgctctccac
    11881 gcacaggggc ggcgcggccg caaggcgaaa gagcgcgtgg acgccgtcgc cgccgcattg
    11941 attctgcaat cctatctcga tgcccaaccg ggagaaaagc gcgatggcga ataagccggc
    12001 ccggaatggt ctgtcgccgt tcggatactt gtggcgtatc ctcgtcgtcg gcacacttct
    12061 cattgtaacg gccgtcgcca gctggatgtt ctaccgaacc tggctcggcg aagggcttga
    12121 tcgtcctgcc gctaatccca acctcagtca gtcccgacgc ctctacctcg aatactatct
    12181 gactgagcgc gccgccgaga tgcagcgccc ggccggtacg gcgaccggtc cccaggctct
    12241 gaccgtttcc cccggcgagg gagccgccgc tatcgcccaa aatctgcaag ccgtcgggct
    12301 attgaccaac acggagctat tcctcaacta tctcacctac tacgggctgg atagcgggct
    12361 tgtcgccgga caatacacga ttgaaccagg gctgacgatc ccccaactgg tcgaggccct
    12421 tggcgcaggc ggagcgcggg cgctggaact cagcttcctg cccggctggc gcagcgaaga
    12481 gatggccaac tacctgcgcg tcgtcactcc cggccaaatc gacggcgatg cctttcttga
    12541 tatcgtccac aaccctcacc gtaatgacct gagcaatttc ccattcttct cgtctttgcc
    12601 cgacggagcc acgctcgagg gttatctatt tcctggagcc tactcgatcg agccggccac
    12661 cgacagcgca gcattggtgc gcatgatgct cgaacgcttt gaccagcagg tcacgccggc
    12721 catgcgtcaa gcctatggcg cgcaggggct gtcgctgcgc gaggcgctca tcctggcctc
    12781 aatcatcgaa aaagaggcga tgctggctga agaaaaaccc atcatggccg ccgtcttcct
    12841 caaccgcctg cggtcgggca tgcccctgca ggccgatccc acggtgcaat acgccctcgg
    12901 ctatcacgcc gaaaccgaca cgtggtggaa atcccctctc gacgcggccg atctggacat
    12961 cgcctcgccc tacaacacat accagatcaa cggtctgccg cccggcccca tctccaatcc
    13021 gggactgtcc tccctcgaag ccatcgctgc tcccgcggac gcggacttcc tcttctttgt
    13081 tctcgattgc gcggcagcca cgacggggcg ccacatcttc agcgcaacct acgaggagca
    13141 tctggccaat gtcgaacgtt gccgctagag cgctcctagt cttgttggcg accctccttg
    13201 tcactgggtg tgcatcagcc gatcccgtcg tcaagatcgg gcttgtggcc cccttcgagg
    13261 gccgggatcg ggcaatcggc tatgatgcca tctacagcgc ccggctggcc gtgcgacaga
    13321 taaacgaggc aggtggtgtc gccggccatc gcgtggcgct cgtggccctg gatgatcgcg
    13381 gcgaccctga gctggccaaa caggcggccg catcgctggc cgtcgatccg ggcgtcgtcg
    13441 tcgtgctggg tcatttcctg ccggagatca cgactatcgc ggctcccgtt tatgagcaac
    13501 accagctggc gctcttgcca atgggcgaga cgccattcgc gcccgtctcg cccaactcgc
    13561 tgccgccgga atttttggga gcctatgagg ccattacgcc tttcgacgaa atcgccggcc
    13621 cctttgccgg accaaccttc gacgctatgg gactggttcg cctagcctta gcagaggctg
    13681 aggaaagcac aggcagcata tcccgcgcat ctgtccagga agcgctcgat ggattaaaat
    13741 accagggact aacgggggag gtctatctgc cctgacaaac ctgacatttc cgacgacggg
    13801 acgccaatca tgattattcg acgaatcctc ggccttatca tgttactcac cggtctgacc
    13861 ctgctggcgg catcgctggc gggcgcttat ttcgttggcg gcgttttgaa tcggttcgcc
    13921 gacagcgtcg gccgaaccgt ggatgtcgcc tcaaccagcc tggacacggc gcgaggcaca
    13981 ctggaactgg cccgcgattc gctcggcgat gtcgatggcg ggttggcgac ggccgtcacc
    14041 gccaccgcgg gggcagcccg aacgttggac gagagccgcc ccttactgga taacgtggcc
    14101 atcgtcacca cccaggaaat tcccgaagcc atcgagggca tgcaaaccgc actgcccaac
    14161 ctgatcgagg tagcgggtgt catcgacagg accctgacga ctctaagttc cgtcggcatc
    14221 gatcagacta tccccctgcc cttcggcggt tcaatcccgt tgaagtttga tctgggcatc
    14281 gactataacc ctacgacgcc gtttgatgag tcgctgcgcg tgttcgaagc cagtctcgtc
    14341 ggcctgcctg aaagcctgcg cggtcttgag gatgatttgg cgacaaccaa tgccaacctc
    14401 gcatcgctct cggccgactt gcaggctaca tcggataatc tggcgacgat caatacccgc
    14461 atgggcgatc tgagcgattt gttcgaccaa tacgtgctgt tggtcgacaa tgtcaccggt
    14521 ttgctggcgc agcttgaaac ccaattggcg ccccggctgg aactgatacg cattgtggcg
    14581 atcgccttgc tgatcgcgct tggcctgagt caactcgcgc cgatctacct ggggtgggag
    14641 ctactgaccg gccggcgcga tccgcaggct cacatcaaca atgtagctgt cgtctctgac
    14701 gtgccgcctg ccttcgctac ttccgatata ccaacgtcca attactcggc cgatctgcac
    14761 cctgattcgg cgcaaagccc cggcgcagcc accggcgaag agacccatgc ccaaacctaa
    14821 aagcaaggcg gtcactctgt cgctcgcgct cttcgcgctc atcgccgccc tgctcacagc
    14881 aggctgccgc ggccaggcat cgaccgaacc gacagcccag gtaccgtctg atgtgccggg
    14941 gctcctgcct acacctaccc tggccgcgcc ggggagcgtg gatccattgc ggccgccgac
    15001 gcccatcgtc atgccctcgc cgaccccgtt gtcaagcgat gtcatcgaga cgccgtccga
    15061 gatggagcag cctaacctcg aagccccgct cgaagttcag cccaccccaa ccgtcacctt
    15121 acccgaaggg gccacacctg ccgagctact ggtgctgggc caaaccgccg cggccgaagg
    15181 ggatcacctc atgtccgccg ccgcctttcg agccgtagtt gaccggccgg gcgatctggc
    15241 cacgggcgaa atcgctacag cgcgcttggg gctggccgtg gccttgatgg cgctggggga
    15301 tcacagcgcg gcggccgtcg aactggaggc ggtcgttcag gaaccgcagt tcaccgcaga
    15361 tcccggcaag ggtggcgcgc tgctggcgac agttaacacg gccgatgtgg ccgcttttca
    15421 tctgggccag gttcgcgccc gcatgggtga tcacgccggg gccatcgatg cctaccgcgc
    15481 ctacctgcaa aataaccccg acatggcggc ctatgttgag ccattgatcg ccaacgccta
    15541 cgaagcgttg ggcgacaccg aaggggtgat cacagcgctg gaaggtgccg taaccggccc
    15601 ggcccaacga tttaaggctg tggcgaatcg aatgcgtctg gcggagcttt atctcgcccg
    15661 gcaggaatac gccaacgcca ttgcccaata cgacgccgta cacgacatcg cccgcaccga
    15721 ggcgaccaag gggcagatga cctatctcgc cggacgggcg gagatcatgg ccggcaacac
    15781 cgccgcgggc cacgaacgct tccagcgcgc catcaccgat tatccgcggg cggccgagag
    15841 ctatcaggct ctggtcgctc tggtcgacgc cggaatcccg gtcgatgaat accagcgtgg
    15901 cgttgtcgat tattacgcgg gggcctatct gcccgccgtc gaggcttttc gccgctacct
    15961 gtccggggca ggagacgaat acaacaccga cgcccacgtc ttcctggcct ggagcctgga
    16021 gagattgggc gacgttgaga acgccctggc cgagcttgat accctgacca agcgtgatcc
    16081 cgcccgcgcc ttgtttgaac gcgccggaat ccttcatcga gccggccggc aagacgaggc
    16141 gctggcagcc tatgatgagt ttcttgagag tcatggcgac acggaaggcg cggccggcgt
    16201 tgcctggtcg gccgctgcgt tggccgacga gatggcccgc gacgatacat ctgcccgcta
    16261 tcttttcctg gcggacacct tcccattcga tgagaacgca cctctggctc ttttccgcgc
    16321 cgccgatctg ctgcaagcgg gtggcgacga cgcggaagca acggccctgc gactgcgact
    16381 ggtcgagcaa tacccagcca acatcttcgg cgcggaggcg cttttccgcc tgctccaggc
    16441 ggccgaaagc gaccccactg gtgagccaac tcgcgccgaa ctgatggagc aggtggcttc
    16501 gctctcgccc acgaactatt tcgccctgcg cgcggccgat cttgcggccg ggatcgcgcc
    16561 cttcaccgct gccggtccga tgaacctggc catcgacccg gacgaaggcc gcgcggaggc
    16621 cgaggcctgg ctccgcgagc gactgatcgc cgacggcgtc gaggtgccgg caggcgaact
    16681 gggcgatctc tcgctcgatc tggctgccga cccgcaccgc atcatcggtg agaagttgtg
    16741 gcagcttggc ttgcaggaag aagccaaagc cgagctggaa ctggtgcgcg agtcctacgc
    16801 cggtgatccg ttggcgactt accggcttgc gcgcttcttt ggcgagttgg ggttgtaccg
    16861 ttcgtcgatc atcgccgcgg ccgggctgct gcgcgattta ggcgcaaccg ttttcgacgc
    16921 gccactatac ctcggccgcc tgtcctatcc cgtctattat tccgatctca tcatgccgct
    16981 ggccgagcga tatgacttcg acccgcgcat acagtttgcg ctggtgaggc aagagagtct
    17041 gttcgagagc ttcgcccggt caggcgcggc cgcgcagggg ctcagccagg tgatccccga
    17101 caccggcgcc tggatcgccc aaaggttggc ctggcccaac ttcgtgaacg aagacctcta
    17161 taagccttat gtcggtctca acttcggcgc ttattacctg agccaacagc tgcacaactt
    17221 cgacggccac gtccacgccg ctctggcggc ctacaacggc gggccgggca acgccgcgcg
    17281 ttggctcgag gtggccggag tcgaccttga ccgctttgtg gatgcggtcg attttccgga
    17341 gacgcgcctc tacattgagc gcatttatga agggtataac gcttatcgcc atctctatgg
    17401 cggtccctag cccaaaaagg gtatactatt tatggtagta tggtcgtgga gagtggtcac
    17461 cccgtaaatt ggcagaggca caccacttct caggccaggc tcgaaatgac gatttacaaa
    17521 attccggtct ttgatagcac agtctgcgct gccgccaggg tcaacgagga acaaccccat
    17581 gcccaccaat tttgactggc agaccgaaga agacaatcgc cgtcgccccg aatccttgtg
    17641 ggacgactct tccgggtcgc ccgccgggcc acccgcgcga cggcgaccat ggcgaatgat
    17701 tggcctgatc gccaccctcg tgctcatcgt tggatcggtt gtctggtggc gagtcgacaa
    17761 gtacatcgat gcaacgctgc aagccaatcg caacgacgtc atcgccagcc ataatctcct
    17821 gcaacgcgcc gtggccgata gcgatgaaga ggtttttcgc tccgtgctct ccggccgaat
    17881 gccggcctgg accgacgccg aactggatgt tttctaccgg caactggtct ttgaccgcgg
    17941 cccgctgggg ctgacgccgg ttgaagggtc gctgccggtc attttgtcca caacggaggg
    18001 agaggtcgac gccgatcagc gaacggccga catcgagctt tcacctgata tgaacgaagc
    18061 cgttgttgtc gtcgatcacc cttttcaggt cacggctact ggtgaaacag tcgttctgcg
    18121 acaaacctca atctttcgtc gggggggttc ccgctggctg ctctctccgc cgttggatga
    18181 attctggggc gactggataa cggtcgaggg agaatatcta agcctgatct atcccgaccg
    18241 ggatgctcca actgccgcaa ggttggccgc cgatctggac gaggagatcg gccgcatgtg
    18301 cgccaccctt gaagagatca actgttccgg cgatctccac ctgacggtta ggctggacac
    18361 cgatccgacc agcctcgccg c
//
LOCUS       DAOMQG010000027        18256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1344_length_18256_cov_11.003681, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000027 DAOMQG010000000
VERSION     DAOMQG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 18256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18256
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1344_length_18256_cov_11.003681"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLC06_03070"
     CDS             complement(<1..758)
                     /locus_tag="PLC06_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRO89458.1"
                     /translation="MSKILGIDLGTTNSVVAVMEGGEPVVITTAEGGRLTPSIVAFTK
                     NGERLVGQTAKRQATINPENTIFSVKRFIGRHFDDPETMEDRNRLPYPIEKGPQGDVR
                     IRVPVKNQTYTPQEISAMVLAKMKKDAEDYLGQPVTKAVITVPAYFNDSQRQATKDAG
                     RIAGLEVLRIINEPTAAALAYGLDKQKDERILVFDLGGGTFDVSILDVSEGLIEVVST
                     HGDTHLGGDDWDLAIVDWVTSEFLRDQGIDLKQDR"
     gene            1040..>1794
                     /locus_tag="PLC06_03075"
     CDS             1040..>1794
                     /locus_tag="PLC06_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRO89459.1"
                     /translation="MSINWQPPNDWTCITSIDAHTAGEPLRIITGGLPPIPGDTILAK
                     RRYAREHLDHLRTALMWEPRGHADMYGCILTEPTTPDGTLGVLFLHNEGFSTMCGHGV
                     IGLVKVGIETGLLAADPVVRLDTPAGRVTAYPHYDPAGKVTHVSFHNVPSFVYALDQV
                     VDVPGLGRVRYDLAFGGAFYAYVSAEALGLNLTPDDFRRIIDRGTRIKQAIIAAGTPQ
                     HPFEPDMSLLYGTLFVGPAHDPANHSREVCVFA"
     assembly_gap    1795..1894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1895..2593)
                     /locus_tag="PLC06_03080"
     CDS             complement(<1895..2593)
                     /locus_tag="PLC06_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRO89460.1"
                     /translation="MSSPTLATPFSFSTPQHFYDAYIFDLDGTCYLGDALLPTVLETI
                     TRLRDMDKRTVFLSNNPTQTREAYAERLTRLGLPTPASDVVNSSLVMVDFLSERMPGA
                     RLFVVGEASLERELSAAGFDLADSAEGVAAVIASFDRAFNYHKLQVAFDAIRAGARFF
                     ATNADRFCPVPGGGQPDAAAMIAAIEASTGVRVEAVVGKPSRYMADAILHFLGLPPER
                     CIMIGDRLETDVLMG"
     gene            complement(2662..3360)
                     /locus_tag="PLC06_03085"
     CDS             complement(2662..3360)
                     /locus_tag="PLC06_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89461.1"
                     /translation="MSRTKSILMIFVLFSAFLVACGSAANGPASGTPLPEATEPPTLV
                     APEEIEDPAEEDGSGAIIAVVVPYLAQELGVAEDKIKATEIVEAEFSDSCLGLGGPAE
                     SCLQAITPGWLILADVDGTSYEVHTDATGEQIRIGPTLPAESDAPAPSDTAAAAAQEF
                     LATQLDVALGDVQVMSVQAMEFSDSCLGFGGPDESCLQVITPGWIVILDVGGKTYEAH
                     TDEAGQQVRLAPTM"
     gene            complement(3591..3663)
                     /locus_tag="PLC06_03090"
     tRNA            complement(3591..3663)
                     /locus_tag="PLC06_03090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Asp,seq:gtc)
     gene            complement(3751..>4554)
                     /locus_tag="PLC06_03095"
     CDS             complement(3751..>4554)
                     /locus_tag="PLC06_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRO89462.1"
                     /translation="ANMYRNGGFRMRYDVLRDFACRDGAEPVRLNAYVSFDADRGRTD
                     SVYQKSTQRFYSLLRDFGFKVMIKEVRWYEDENGKRYGKANADLDLAVDMLLQSENLD
                     RVMLVTGDGDFTRVVSAVQNRGCRVEVVALDNASADLRRESDLFISGYLVPDLVPTFT
                     NGGSFDDEKPWGEISSVVRGTCYFHKQNYGFMRFLRDSGPKLWLTDTQNPDSPYETAY
                     FNDSSLPDNVDPLNLPSYNHIFQFELAESRHPTGKFEARNIQFLSWVPT"
     assembly_gap    4555..4654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4709..5887
                     /locus_tag="PLC06_03100"
     CDS             4709..5887
                     /locus_tag="PLC06_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRO89463.1"
                     /translation="MQKATRQQTKGHNSRLVLRTIYNGGELSRADVARLTGLTRPTVS
                     TIVAELLESTLVIETGQGPSAGGKRPTLLSINGNSRYLIAVDLSGTNFRAARLNLRGE
                     IEARAALPAAGIHGDDVITAIHELVQEITPLKGDLLGIGVATPGLVDPNNGIIRRAVN
                     LGWVNIPLRDLLEERYHRPAHIANDSHMAALAEFTYGEPIDSDNLIVIRASRGVGAGI
                     ILGGLPFYGDGFGAGEIGHVVVDIDGDPCTCGNRGCLETTSSVNAILHQLSIADGVHA
                     SPAGHPSPNWNQFVTAVADHDPVATDIAVTAGRHLGVAVANLVGAFNIQTMVLAGRVA
                     DLGDILLDAVKAELHQRVLPSIAGMTTIRLSSLAPDRVGDIIILGCSSLLLQRELGVL
                     "
     gene            5884..6894
                     /locus_tag="PLC06_03105"
     CDS             5884..6894
                     /locus_tag="PLC06_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRO89464.1"
                     /translation="MSLRLGLDIGGTKTAALVVDESGAPLGQATKPTDVTSPERLVTG
                     TLRIIDEALQDASSDRSDLLAAGIGIPGQVDPADGSVRLAVNLNLREPYPLGRVLQDA
                     LGVPVALENDVRAAAWGAYHWACEQAPLNSLAYLSIGTGIAAGLVLEGRIWRGSNGMA
                     GEIGHIPMNETGPRCVCGSYGCLESIAAGPAIAAQAAAAGITIHGRPPLTHEVYSLAA
                     AGNPEAAEVVDRAAGYLARAVYLLFMTYDVDQVVLGGGVTRAGDAFERPLRHALSMLR
                     SNSALVSSMMPDGKITIAPVDFNAGVIGAVHLAPRPRRISRREEDEENKEPLPLSKIE
                     SV"
     gene            6936..8402
                     /locus_tag="PLC06_03110"
     CDS             6936..8402
                     /locus_tag="PLC06_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89465.1"
                     /translation="MKRFMLVVLLVALTAMMVACGGSAAVQQVAPTVEAAVQQVAPTV
                     AAAIAPTEAPAPTEAPAPTEEPAPTEAPVAEGTFLERAKAGEFKGTTVSLWGVMVDEE
                     ATKMELALAPFEEATGIDVVYEGSKEFETQINVAVDAGSPPDVANISQPAMVQRFATA
                     GALVDVSTFLPREKLQETYIDSWIDMSTMPGADGNDIIAGVWQRASAKSMVFYPKAQF
                     DAAGYVVPETWDEMLALTQQIADDGDTAWCIGIESGAATGWVATDWMENIMLRTTSLE
                     NYDKWVTGELPFASPEVKAAAERMAEIWLNDEYVYGGVPSIVSTFIGDSPVPMFTDPP
                     GCWFHAQAAWITSFFGNDALVAGEDYDFFYLPPIDESLGRPALVAGDMMVMFNDRPEV
                     RALMEFFSKAEGVEGWVRAGGTLSPHKDADLDWYTTYTDRKVAEMLLNATSVRFDASD
                     LMPAEVGSGSFWKGMTDWVSGATDLDTALAEIDASWPK"
     gene            8543..10117
                     /locus_tag="PLC06_03115"
     CDS             8543..10117
                     /locus_tag="PLC06_03115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HRO89466.1"
                     /translation="MIPKTEDLIGYKLMLAFTGHMPPARILDWIAHRPIGGFSLFRPH
                     NYDNPAQVRELTAALQRAAREAGQAPLLIATDQEGGQLAAMGAGTTQFAGNMALGATR
                     DPELALRVGRAIGLELAAMGVNVNYAPVLDLNTNPRNPSLGIRSFGDEPGPAAALGAA
                     MVVGMQSAGVAATLKHFPGKGEAAVDSHYKMPVIAHSRERLDRIETPPFKAGIDAGAK
                     LLMTGHFAIPALTGTSAYPATVARRVMTDYARGELGFEGVIITDALDMGAITQGAGQI
                     IDVIAAVRAGVDLMLVMHDVDTQERLYGGLVLAYTRELIDESHIKPSLDRILTLKEWA
                     AAQPQPEIDVVGCAEHRALEIELARRSITLVRDEAGLLPLRLAPDARIAAVMPAPKDL
                     TPADTSSTITPHLASALRAHHPHVDEFVTEHLPGDTEIAALRQQLADYDLLVLGTINA
                     SMQPEQAVLANELLGLGIPTVTAALRTPYDLASYPAARTHLCTYSIQPASLDALAAAL
                     FGEFAPTGQLPVAALH"
     gene            10123..11280
                     /gene="nagA"
                     /locus_tag="PLC06_03120"
     CDS             10123..11280
                     /gene="nagA"
                     /locus_tag="PLC06_03120"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRO89467.1"
                     /translation="MFAYRHATLITPDIVIEDGSLVIDGDHIQAVGGPDLVLPTDIEF
                     RDATGLILAPGFIDLQVNGGFGLDFTADPTTIWDVAAGLPRYGVTSFLPTVITSPPST
                     VQQAQDILMAGPPSGWSGARPLGLHLEGPFLNIAKKGAHNPAYLRAPSLQAVEGWSPA
                     SAVRLVTLAPELPGATEVIEALAARGVVVSAGHSMASYEAAEAGFAAGARYSTHLFNA
                     MPTLHHREPGLVAAALADPHVTVGLIPDGLHIHPALVKLVWQLLGRGRLNLVTDAMAA
                     MGMSPGEYLLGDYQVTVDGTSARLPDGTLAGCVFSLDAILRAFRAYTGASLADTVATV
                     TTTPARLLGVDDRLGLLRLGAAADLVLMTPNGDIIQTIVAGKLLYQQEKTG"
     gene            11448..13931
                     /locus_tag="PLC06_03125"
     CDS             11448..13931
                     /locus_tag="PLC06_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89468.1"
                     /translation="MKTTRHLLIGIFLISMGALALEVVLTRIFSVTMWYHFAFLAISI
                     ALMGSATAGVFIYFFPRLSKPDMAQKWIGRAAVALAIAVPAVFLIYLRIPFRPVLMNR
                     DGAFSFEQIGWLALIYILLAIPFFLSGSVLALTLAGWPNDAGKVYAADLIGAAIGCLV
                     SIAALSQLGGVNALFLLSLILALAASAFIYGSRRWTITAGVIILITGGLLVSNIASPW
                     TRIVVNKAGGEEPPIVYEKWNIHSRVTVYEPDVFPFFWGISPQKWEETIAAGGNWNHA
                     LLLIDAVAGTPIQSFNGDLGTVQFLRSDLTAIAYQLMDDPETLVIGPGGGRDVLAALA
                     SGAPHVTAVEVNPAIIEAVKGQFADFAGHLYDRPDVSVVVADARGFIDRSPGDYDVIQ
                     ASLIDTWAAGGSGAFALSENSLYTKEAFTSYFNHLSDDGVLSISRWYLPDRPAETLRL
                     VTTGLAGWEDAGVADPRDHIAVIALFDSTRATTEGLSTTLFKRQPFTPEEVSRLQEVA
                     SSYGYQVIYAPGLEPFEEVGEFIAGDDHAGFIANYPLDISPATDDRPFFFNLILLGDL
                     FDPALSGSGVYRTSMEAIYILFAVIGVTATLSALFILVPLWLGAKRRNLARPSAMTLA
                     YFGALGMAFMMVEIPTMQKLTVYLGQPVYSLAVVLFSLLLFSGLGSWWSGRWAAQSIS
                     ADMRRVFPVLIGFLILHAVFSTFVLRQTLQFDLAGRLVVAVLLLSVMGFMMGIPFPVG
                     IRWVGRRQSSVVPWLWGINGVTSVLGSALATALSIHIGFRLTLVVATLVYGAAAMLFL
                     NILKQTSEESESIDPVLPSGPHSADLGPA"
     gene            13990..15042
                     /locus_tag="PLC06_03130"
     CDS             13990..15042
                     /locus_tag="PLC06_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRO89469.1"
                     /translation="MTTLQHTHLYREIHEQPNVVRRLLDKEMATAQALADLIHGRGID
                     HVVIAARGTSDNAGRYAQYVWGAMNGLSVGLAAPSLFSIYHRPPRFGNALVIGISQSG
                     KSPDIVSVLAEARRQGALTAALTNRPTSPLAGEADVVIDLQAGEELAVAATKTYTAEL
                     AAVALISAALSGTAEQRDALLTVPEAMEATLAMNDAVAEIAPRYRYMQRCVVVGRGFN
                     LATAFETALKLKEMTYTIVEPYSSADFLHGPLAMIEQGFPVILIAPSGVMAAEMLDFA
                     RTLRERQAELIVVSDAADLLDTARVPLKLPVPMPEWLSPITSILPGQMMAMHLAHTRD
                     YNIDAPRGIMKVTETR"
     gene            15451..16809
                     /locus_tag="PLC06_03135"
     CDS             15451..16809
                     /locus_tag="PLC06_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89470.1"
                     /translation="MSNKRLWLPILLMVLALMIAACGGTAPTETTAPTAAPAPAEEEA
                     APAEEEAAPAESTGANQLEFYSWWTAGGEADGLNAMYDLFKAMYPDIEIVNATVAGGA
                     GSNAKAVLATRMQAGDPPDSFQVHAGHELIDTWVVADKMEPVTFIFEENGWMDAYPPD
                     VISILSYNGEIWSVPVNIHRSNVMWYNPAVLEANGLTAPTTIEEFFTVADALKAAGVT
                     PLALGDNGIWTATHLLESVLLGTLGADGYRGLWTGDTDWNSPEVTTALESFARMLDYV
                     NADHAALSWDQAAQLVADGDAAMTIMGDWANGYFTSIDLKPNVDYGYAPSPSTDGIFM
                     MLSDTFSLPKGAPHRDNAIKWLTLAGSKEGQDAFNPLKGSIPARTDGDRSLYDEYLQS
                     AMDDFASNAITPSLAHGAAASEGWVTAFNDIMTLFVTDRNVENAQAAMAKACVDAGVC
                     GQ"
     gene            16951..17889
                     /locus_tag="PLC06_03140"
     CDS             16951..17889
                     /locus_tag="PLC06_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRO89471.1"
                     /translation="MKSQSRNERWIAFLMILPSIILLGIFVYGFIAWTGVVSVSKWDG
                     IQPDYTFVGFNNYKQLFSLALGGISAIRFHIDLWNTFFFTIFFLFLCLIFGLLLAILL
                     DQKIKGEGIFRTIYLFPMAISFVVTGVVWQWIFAPGTESRARGVNALLQSLGLDNLTW
                     GWYTDPTRIGPFHVALIPVIIAAAWQMIGYTMAMYLAGLRAIPDDLREAARVDGAGSF
                     GVYRHVVLPLLAPITLSALIILGHISLKIFDLIYTMTGKGPAFATDVPGIFMFETTFQ
                     GNHYAQGAAISIIMLLMVALIIIPYLIYSFRQEVEL"
     gene            17886..>18256
                     /locus_tag="PLC06_03145"
     CDS             17886..>18256
                     /locus_tag="PLC06_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRO89472.1"
                     /translation="MTARFNPARFAVYLLLILFALIFLIPVYVLLSSSLKTFAEVQDL
                     SRLWAPPQGLNLESFKAAWAGIPEKGLRGLAPNLMNSFKLTIPATILSALLGSMNGYV
                     LSKWKFPAANIVFPLMLFGMF"
BASE COUNT         3438 a         5786 c         5228 g         3604 t
ORIGIN      
        1 cgatcctgct tcaggtcgat gccctgatcg cgcaggaact cgctcgtcac ccaatcgacg
       61 atggccaggt cccagtcgtc gccgcccaaa tgggtgtcgc catgggtgga tacaacctcg
      121 atgaggcctt cgctcacgtc cagaatggac acgtcgaacg tgccgccacc caggtcgaag
      181 accaggatgc gctcgtcctt ctgcttgtcg aggccatagg ccaatgcggc ggccgtcggc
      241 tcgttgatga tgcgcaggac ttccagaccg gcaatacgcc cggcgtcctt cgtggcctga
      301 cgctggctgt cgttgaagta ggccggcacc gtgatgaccg ccttggtcac cggctggccc
      361 aggtaatctt cggcgtcctt cttcatcttg gccagcacca tcgccgagat ctcctgcggc
      421 gtgtaggtct ggttcttcac cgggacgcgg atacgcacgt cgccctgcgg ccctttttcg
      481 atgggatagg gcagacggtt gcggtcttcc atcgtctcag gatcgtcgaa atgacggccg
      541 atgaaccgct tcacggaaaa tatcgtgttt tcggggttaa ttgtcgcctg gcgcttggcg
      601 gtctggccga ccagccgctc gccgttcttg gtgaaggcga cgattgatgg agtcaggcgt
      661 ccgccctcgg ccgtcgtgat gacaacaggc tcgccgcctt ccatcaccgc gaccaccgag
      721 ttagtggtgc ccaggtcgat tcccaggatt ttgctcattt ttcccttcca gtggtcagtg
      781 gccggtggtc gatgatcagt cgatcacgcg caccgacgac cttccaatta attctttctt
      841 tagactttct ataccataca gaatcggcgt aagatttatg ttggcgcaac gtttgcgtta
      901 catatgcaat catgaagaaa gaatccacag attacacaga ttacacagat tgaagatcta
      961 ctcttcaaaa tctgtgtaat ctgtggattt cttcccgtct ttgcgccttt gcgttaaatt
     1021 ctctctcagg agataaccca tgagcatcaa ctggcaacca cccaacgact ggacttgcat
     1081 cacttccatc gacgcccaca cggccggcga accgctgcgc atcatcaccg gcggcctgcc
     1141 gcctatcccc ggcgacacga tactggccaa gcgccgctac gcccgcgaac acctcgacca
     1201 cctgcgcacg gccctcatgt gggaaccgcg cggccacgcc gacatgtacg gctgcatcct
     1261 gaccgagccg accacccccg acggcacgct cggcgtcctc tttctgcaca acgaggggtt
     1321 cagcaccatg tgcggccacg gcgtgatcgg gctggtgaag gtcggcattg agacggggct
     1381 gctggcggcc gatcccgtcg tgcgcctcga cacgcctgcc ggccgcgtca ccgcctatcc
     1441 ccattacgac ccggcgggca aagtcaccca cgtctcgttc cacaacgtgc cgtcgttcgt
     1501 ctatgcgctc gatcaggtgg tcgatgtgcc cggcctcggc cgcgtgcgct acgacctggc
     1561 cttcggcggc gcgttctacg cctacgtgtc ggccgaagcg ctgggcctca acctgacgcc
     1621 cgacgacttc cgccggatca tcgaccgggg cacgcgcatc aagcaagcca tcatcgccgc
     1681 cggcacgccg caacatccgt tcgagccgga catgagcctg ctctacggca cgctcttcgt
     1741 cggcccggcc cacgacccgg ccaaccacag ccgcgaggtg tgcgtcttcg ccgannnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccat cagcacatcc gtctccagcc
     1921 ggtcgccgat catgatgcag cgctccggcg gcaggccgag aaaatgcagt atcgcgtcgg
     1981 ccatatagcg cgacggcttg ccgaccaccg cctcgacgcg caccccggtc gaggcctcga
     2041 tggcggcgat catcgccgcc gcgtccggtt ggccgccgcc cggcacgggg cagaagcggt
     2101 cggcgttggt ggcgaagaag cgcgccccgg cgcggatggc gtcgaaggcc acctggagct
     2161 tgtggtaatt gaacgcccga tcgaagctgg cgatgaccgc cgccacaccc tcggcgctgt
     2221 cggccagatc gaacccggcg gcggacagct cgcgctccag cgaagcctcg ccgaccacaa
     2281 acagccgcgc gccgggcatg cgttcgctca ggaaatcgac catgaccagc gatgaattga
     2341 cgacgtcgct cgccggcgtc ggcagcccca gccgcgtcag gcgttcggca tacgcctcgc
     2401 gggtttgggt tgggttgttc gacaggaaga ccgtacgctt gtccatgtcg cgcaggcgcg
     2461 tgatggtctc cagcacggtg ggcaacaggg catcgcccag atagcaggtg ccgtcgaggt
     2521 caaagatgta agcgtcgtag aaatgttgtg gcgtcgagaa agaaaacggt gttgcgagcg
     2581 tgggcgaaga catcgttatc gtgcggatgg gagcggaaat gccgctccca tccggcattt
     2641 attccaaatt gcggctaccg gctacatcgt gggagcaagc cgcacctgtt ggccggcctc
     2701 gtccgtatgg gcctcatagg tcttgccgcc aacgtcgagg atgacgatcc agcccggggt
     2761 gatgacctgg aggcagctct cgtccggccc accaaaaccg aggcaggagt cgctgaactc
     2821 cattgcctga accgacatca cctgaacatc gccaagggcc acatccagct gggtcgccaa
     2881 aaactcctgg gccgcggccg cggccgtgtc ggacggagca ggggcatcgc tttctgcggg
     2941 cagcgtgggg ccaatgcgaa tctgctcgcc cgtcgcatcc gtatgaacct cgtaggatgt
     3001 gccgtccacg tcggccaaaa tcagccatcc gggggtgatc gcctggaggc agctctcagc
     3061 cggaccgccc aatcccagac aggaatcgct gaactcagcc tccacgatct ccgtggcttt
     3121 aattttgtcc tcggcaacgc ccagttcctg cgccaagtac ggcacaacga cggcgataat
     3181 cgcgccggag ccgtcttcct cagcggggtc ttcgatctct tcaggggcca ccagggttgg
     3241 cggctcggtc gcctcaggta agggcgtgcc ggacgcagga ccattcgcgg ccgacccaca
     3301 agccaccagg aatgcgctaa aaagcacgaa tatcatcaaa atcgactttg tacgtgacat
     3361 cattgtttac tccttttgcc gggatcgtca tgactgtctg attaaggaaa gtataaccgg
     3421 tcaaccggac atatcagtat aacgctcaca aaaagcaaaa ggttccaccc ggatcgcgca
     3481 gttaaataat gccggatgcg gcctcgcaga aggatttaac tctatcaggc aacaaaaaag
     3541 gacctctttg gctgaggtcc tcttgtccta tataaattcg ctataggcta cgggagcgac
     3601 ggggatcgaa cccgcgatct tcggcttgac aggccgacgt gttaaccgct acaccacgcc
     3661 cccatgcgtg aatcaggatg ttatcagaag tgccataagc tgtcaatatc tcgcagcctc
     3721 atttttggcg ggtatccagg ccttccaatt tcaggtcggc acccagctga ggaattggat
     3781 attccgcgcc tcgaacttcc ccgtggggtg gcgcgactcg gccaactcaa actgaaagat
     3841 gtggttatag ctgggcaggt tcaagggatc gacgttatcg ggcaggctgg agtcattaaa
     3901 gtaggccgtt tcatagggtg agtcggggtt ctgcgtgtcg gtgagccaca gctttgggcc
     3961 ggaatcgcgc aggaagcgca tgaagccgta gttctgcttg tggaaatagc acgtcccgcg
     4021 cacgactgaa ctgatctccc cccatggctt ttcatcatca aaggagccgc cgttcgtgaa
     4081 cgtgggcacc aggtcgggca cgaggtaacc cgagatgaac aggtctgatt cacgccgaag
     4141 atcggccgaa gcgttatcga gcgcaaccac ctctacccgg cagccccggt tctgcacggc
     4201 gctcaccaca cgggtgaaat caccgtcacc ggtgacgagc atgactcgat ccagattctc
     4261 cgattgcagc agcatatcaa cggccagatc gaggtcggcg ttggccttgc cataccgctt
     4321 gccgttctca tcttcgtacc agcgcacttc cttgatcatc accttgaaac cgaagtcgcg
     4381 aaggagggaa taaaagcgct gggtactttt ttggtagaca gagtccgtgc ggccacgatc
     4441 ggcgtcaaag ctgacatagg cgttaagccg caccggctct gccccatccc gacaggcaaa
     4501 atcgcgcagc acatcatagc gcatgcggaa gccgccattg cggtacatat tcgcnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctata atatactttg taagttaatt
     4681 taacttacta tcgattgtgg tcactggaat gcaaaaagca acccgacaac aaacaaaagg
     4741 ccacaacagc cggctcgttc tccggactat ctacaatggt ggggaattaa gccgtgccga
     4801 cgtagcccgg ttgaccggcc tcacccgccc caccgtctcc acaatcgtcg ccgaactcct
     4861 tgagagcact ctggtcatcg agaccggcca aggcccgtcg gccggcggca aacgccccac
     4921 gttgttatcg atcaatggca acagtcgcta tctgatcgcc gttgacctga gcggcaccaa
     4981 ctttcgagca gcacgtctca acctgcgcgg cgagatcgag gcgcgcgccg ctctccccgc
     5041 cgctggaatt cacggcgacg acgtgatcac cgctattcac gaactggtcc aggagattac
     5101 cccactaaaa ggcgatttgc tgggaatcgg cgtggcaacc cccggtttgg ttgaccccaa
     5161 caacggcatc attcggcgag ccgttaacct gggttgggtc aatatccctc tgcgagacct
     5221 gctggaagaa cgctatcatc ggccggcgca catcgccaac gacagtcaca tggcggccct
     5281 ggccgaattc acctatggcg aaccgatcga cagcgacaat ctgatcgtca tccgcgccag
     5341 tcgcggcgtc ggcgcgggca tcattctggg cggcctgccc ttctatggcg atggatttgg
     5401 cgcgggcgag atcggccacg tggttgtcga catcgacggc gatccatgca cctgcggcaa
     5461 tcggggctgc ctggaaacga ccagcagcgt caacgccatt cttcatcagc taagcatcgc
     5521 agatggcgta cacgcctcgc cggccgggca tccatcgccc aactggaatc agttcgtgac
     5581 ggccgttgcc gaccatgatc ccgtcgccac cgatatcgcc gtcaccgccg gccgtcattt
     5641 gggtgtagct gtagccaatc tcgtcggcgc attcaacata cagacgatgg tcctggccgg
     5701 ccgcgtcgcc gatctgggcg acatcctgct cgatgcggtt aaggctgaat tacatcagcg
     5761 agtactaccc tcgatagccg ggatgacgac catccggctg tcatcccttg cccccgatcg
     5821 ggtcggcgac atcattatcc tgggctgttc atccttgctg ttgcagcggg agctgggggt
     5881 actatgagtt tgcgcctggg cctggacatc ggtggcacga agacggccgc gttagtggtc
     5941 gatgaatccg gcgctccgct gggtcaggcg accaaaccca cggacgtcac cagtccggaa
     6001 cgtctcgtga ccggcacgct tcgcatcatc gacgaggccc ttcaggacgc gagcagcgac
     6061 cgaagcgatt tgctcgccgc aggcatcggc atccctgggc aagtcgatcc cgccgacggc
     6121 tcggtccgcc tggcagtcaa tctaaacttg cgcgagccgt atccattggg ccgggtcctg
     6181 caggatgcgc tgggcgttcc cgtggcgctg gagaacgacg tgcgggcggc cgcgtgggga
     6241 gcatatcatt gggcatgcga gcaggcgccg ctcaacagcc tggcctatct cagcatcggc
     6301 acgggaatcg ctgccgggct ggtgctggaa ggtcgcattt ggcgcgggtc gaacggcatg
     6361 gccggcgaaa ttggtcatat tcccatgaat gagaccgggc cacgctgcgt ctgcggttcc
     6421 tatggttgtc tggagtcaat cgccgcggga ccggccatcg ccgcgcaagc ggcggcagcg
     6481 ggcatcacca tccatggtcg cccgcccttg acccatgaag tatattcgct ggcggccgcc
     6541 ggcaatccgg aagccgctga ggtggtcgac cgcgccgccg gttatctggc gcgcgccgtc
     6601 tatctgctgt tcatgactta cgatgtggat caggttgtcc tgggcggggg tgtaacccgc
     6661 gccggggacg cttttgaaag gccgttgcgg catgcgctga gtatgctacg atccaactcg
     6721 gcgctggtgt catcaatgat gcccgacgga aaaataacga ttgcccctgt tgattttaac
     6781 gccggagtga taggggccgt gcacctggca ccaaggccaa gacgaataag ccggcgggag
     6841 gaagatgaag agaataaaga accgttacct ctgtcaaaga tcgaatcagt ataaacatgt
     6901 tgtgaaaatt caatctagtt agtccggagg aaagaatgaa acgttttatg cttgtagttt
     6961 tgcttgtagc cctgacggcc atgatggtcg cctgcggtgg cagcgccgcg gtgcagcaag
     7021 ttgcgccgac ggtggaagct gccgttcaac aggtcgcccc gaccgtcgcg gcggctatcg
     7081 ccccgactga ggcccctgcc ccgactgagg cccctgcccc aaccgaagag cctgccccga
     7141 ccgaagcgcc ggtcgccgaa ggcaccttcc tggagcgcgc caaggccggc gagttcaagg
     7201 gcacgaccgt gagcctctgg ggtgtcatgg ttgacgagga agccaccaag atggaactgg
     7261 ccctggctcc gttcgaggaa gccaccggca tcgacgtcgt ctacgaaggc tcgaaggaat
     7321 tcgagaccca gatcaacgtc gccgttgacg cgggttcccc gcccgatgtt gccaacatct
     7381 cccagcccgc catggtccag cgcttcgcta ccgccggcgc tctggttgac gtcagcacct
     7441 tcctgccccg cgagaagctg caggagacct acatcgacag ctggatcgac atgtcgacga
     7501 tgcccggcgc cgatggcaat gacatcatcg ccggtgtctg gcagcgcgcc tcggccaaga
     7561 gcatggtctt ctacccgaag gcccagttcg acgccgccgg ctatgtcgtc cccgagacgt
     7621 gggatgagat gctggctctg acgcagcaga tcgccgacga cggcgacacc gcctggtgca
     7681 tcggcatcga gtccggcgcg gccaccggtt gggttgccac cgactggatg gagaacatca
     7741 tgctgcgcac gacctcgctg gagaactatg acaaatgggt caccggcgag ctgcccttcg
     7801 cttcgcctga ggtcaaggcc gcggccgaaa gaatggccga gatctggctg aatgacgagt
     7861 atgtctatgg tggcgtgccg tcgatcgtct cgacgttcat cggcgactcc cccgtcccga
     7921 tgttcaccga cccgcccggc tgctggttcc acgcgcaagc ggcctggatc accagcttct
     7981 ttggtaacga tgccctggtg gccggcgaag attacgactt cttctacctg ccgcccatcg
     8041 acgagtcctt gggccgcccg gcgctggtcg ccggcgacat gatggtcatg ttcaacgacc
     8101 gccccgaagt tcgcgccctg atggagttct tcagcaaggc ggaaggcgtt gagggttggg
     8161 tccgcgccgg tggcacgctg tcgccccaca aggatgccga cctcgactgg tacaccacct
     8221 acaccgaccg caaggtagcc gagatgctcc tgaacgccac gagcgttcgc ttcgacgcat
     8281 ccgacctgat gcccgccgaa gttggctccg gttccttctg gaagggaatg accgactggg
     8341 ttagcggcgc gaccgacctg gacaccgccc tggccgagat cgacgcttcc tggccgaagt
     8401 aaacaagtag cttgactcga aatgggtcgc accctgacag gtgcgaccca ttttcacgaa
     8461 tgcaacgaat ggaacggatc cttttttggt aattggatcc gtttcatccg ttacctccgt
     8521 gaaatccctg gttgaaaatc ttatgattcc caagaccgaa gacctcattg gttacaagct
     8581 catgctcgcc ttcacgggcc acatgccccc ggcgcgcatt ctggactgga tagcccatcg
     8641 cccgattggc ggcttctccc tttttcggcc gcacaattac gataaccctg cacaggtgcg
     8701 agagctgacg gctgccctgc agcgcgccgc gcgcgaggcc ggacaagcgc cattgctcat
     8761 cgccaccgat caggaaggcg gccaactggc agccatgggc gcgggcacaa cccaattcgc
     8821 cggcaacatg gccctgggcg ccacccgcga cccggaactg gcgctgcgtg tagggcgggc
     8881 gatcggcctg gagctggcag cgatgggggt caatgtcaac tacgcgccgg tgctcgacct
     8941 gaacaccaat ccacgcaacc catcgctcgg cattcgctcc ttcggggatg agccgggacc
     9001 ggccgccgcg ctgggcgcag cgatggtggt tggaatgcaa tcggccggcg tagcggcaac
     9061 gctcaagcac tttcccggca agggcgaggc ggccgtcgat tcccactaca agatgcccgt
     9121 catcgcccac agccgcgagc gactggatcg catcgaaacc ccgccattta aagcgggcat
     9181 cgatgcgggc gccaaactgt tgatgaccgg ccacttcgcc attcccgccc tcaccggcac
     9241 aagcgcctat ccggccaccg tcgcccgccg cgtgatgacc gactatgctc gcggcgagtt
     9301 gggcttcgag ggcgtcatca tcaccgacgc gctcgacatg ggcgccatca cccagggcgc
     9361 ggggcagatc atcgacgtca tcgccgccgt tcgcgccggg gtcgatctca tgctcgtcat
     9421 gcacgacgtc gatacccagg agcggctata cggcggtctg gtgctggcct acacgcgcga
     9481 gctgatcgac gaaagccata tcaagccatc actcgatcgc atcctcacct tgaaggagtg
     9541 ggccgccgct caaccgcaac cggaaatcga tgtcgtcggc tgtgccgaac accgcgctct
     9601 cgaaatcgaa ctggcgcgcc ggtcgatcac gctggtgcgc gatgaggcag gcttgttgcc
     9661 cttgcgcctc gcacccgacg cgcgaatcgc cgccgtcatg cccgcgccga aagacctgac
     9721 accggccgac acctcctcga ccatcacccc ccatctggcg agcgccctgc gtgcccatca
     9781 tcctcacgtc gatgagttcg tgaccgaaca cctgcccggc gacacggaaa tcgccgcgct
     9841 gcgccaacag ttggccgatt atgatctgct cgtcctcggc acgatcaacg cctccatgca
     9901 gcccgaacag gccgtgctgg ctaacgaact gttgggcctg ggcatcccca cagtcacggc
     9961 cgcgctgcga acaccctatg atctggcatc ctacccggcg gcgcggacgc atctctgcac
    10021 ctacagcata cagcctgcct cgctcgacgc gctggcggcc gctctcttcg gcgagtttgc
    10081 gcccacggga cagcttcccg tcgccgctct ccattaaaag ccatgttcgc ctatcgccac
    10141 gccaccctta tcacccctga tatagtgatc gaagacggat cactcgtcat cgacggcgac
    10201 cacattcagg cggtcggcgg ccccgatctg gtgctgccca ccgatattga atttcgggac
    10261 gccacgggac tgatcctggc tcccggcttc atcgatttgc aggtgaacgg cggctttggc
    10321 ctcgacttca cggccgaccc gacgaccatc tgggacgtcg ccgccgggct gccgcgctat
    10381 ggcgtgacga gcttcctgcc caccgtcatc acctcgccac catcaactgt gcaacaggcg
    10441 caggacatcc tgatggccgg cccgccttcg ggctggtccg gcgctcggcc gctgggcctc
    10501 catctggaag ggccgtttct caatatcgcc aagaaaggcg cccacaaccc cgcctatcta
    10561 cgcgccccgt ctttgcaggc cgtcgaagga tggtctcctg cgtcggccgt gcgtctggtc
    10621 acgctggctc ccgaactgcc gggcgcaacg gaagttatcg aggcgcttgc cgcccggggc
    10681 gttgtcgtca gcgccgggca ctcgatggcg agctacgagg cggccgaggc cggatttgcc
    10741 gccggcgcgc gctattcgac ccatctcttc aacgccatgc ccacgctgca tcatcgggaa
    10801 ccggggctgg ttgccgcggc actggcggac ccgcacgtca ctgtggggct tatccccgat
    10861 ggcctgcata tccatccggc gctcgtcaag ctggtgtggc aactgctcgg ccgcggccgc
    10921 ctcaatttgg tcacggacgc gatggcggcg atggggatga gtccgggcga atacttgctc
    10981 ggcgattatc aggtcacggt agacggcaca tcggcgcgac tgcctgacgg cacactggcc
    11041 ggctgcgtct tctctcttga tgccatcctg cgggcatttc gcgcctatac cggcgcgtcg
    11101 ctcgccgata ctgtggcgac agtcaccacg acaccggccc gcttgctggg cgtcgacgac
    11161 cggctggggc tgcttcgcct cggcgcggcg gccgatctgg tgctaatgac ccccaatggc
    11221 gatatcattc agacgattgt ggccggaaag ctgctctatc aacaagagaa gaccggttaa
    11281 cagaagggag agcggccaag gccccatcgc cgactttcat agtttacgac ttgcagaggc
    11341 gaagctacaa tcagttaccg tagcctggtc ggtcgtgaac gctgcccggc ctacgacaat
    11401 acctcaccct cacggagcgc aaaaggtgaa tccgtgacga gagagcgatg aaaacaacaa
    11461 gacatttatt gattggcatc ttcctcatca gcatgggagc gctggccctg gaagtagtac
    11521 tgacgcgaat cttttccgtc accatgtggt atcacttcgc ctttctggcg atttcgatag
    11581 ccctgatggg tagcgcgacg gccggcgtct tcatttactt cttcccgcga ttgagtaaac
    11641 ccgatatggc gcagaaatgg atcggccgcg ccgcggtagc cctcgccatc gccgtgcccg
    11701 ccgtgttcct catctatctg cgtatcccct ttcgtccggt cctgatgaac cgcgacgggg
    11761 ctttctcgtt cgaacaaatc ggctggctgg ctctcatcta cattctgctc gcaatcccct
    11821 tcttcctgtc cggttcggtc ctggcgctga ccctggccgg ctggcccaat gatgcaggca
    11881 aggtctatgc tgccgatctg atcggcgcgg cgatcggctg tctggtcagc atcgccgcgc
    11941 tgtctcaatt gggaggcgtc aatgcgctct tcctgctgag cctgatcctg gctctggcgg
    12001 catcggcttt catctacggc tcgcgcagat ggactatcac cgcaggcgtt atcatcctga
    12061 tcaccggcgg acttctggtc agcaacatcg cctccccctg gacgcggatc gtggtcaaca
    12121 aggccggcgg cgaggagcca cccatcgttt acgaaaagtg gaacatccac tcacgtgtga
    12181 ccgtgtacga acccgacgtc ttccccttct tctggggcat ctcgccccaa aagtgggagg
    12241 agaccatcgc cgcgggcggc aactggaacc acgccctgct gctgatcgac gccgtcgccg
    12301 gcacacccat ccaaagcttt aacggcgatc tgggaactgt gcaatttctg cgttccgacc
    12361 tgacggcgat cgcttatcag ttgatggacg atcccgaaac gctggtcatt ggtcccggcg
    12421 gtggtcgaga cgtgttggct gcgctggcca gcggcgcgcc ccatgtgacg gccgtcgaag
    12481 tcaacccggc catcatcgag gccgtcaagg ggcagttcgc cgattttgcc gggcatctct
    12541 atgatcgccc tgacgtgagc gtcgtcgtcg ccgacgcccg cggtttcatc gatcgcagcc
    12601 cgggcgatta cgacgtcata caggcttcgc tgatcgatac ctgggccgcc ggtggttcgg
    12661 gcgcgttcgc cctgtcggag aacagcctct acaccaagga agctttcacc agctatttca
    12721 atcatctcag tgatgacggc gttctgagca tctcccgctg gtatctgccc gaccgcccgg
    12781 ccgagacgct acgattggtg acgaccggtc tcgccggctg ggaagatgcg ggggtggccg
    12841 acccgcgcga ccatatagcc gtcatcgcct tgtttgactc gacccgtgca accacggagg
    12901 ggctatccac aacgctcttc aagcggcagc cattcacgcc ggaggaagtc tcccgtctgc
    12961 aggaggttgc gtcttcctac ggctatcagg tgatctacgc tccgggtctg gagccgttcg
    13021 aggaagtcgg cgagtttatc gccggtgacg atcacgccgg gttcatcgcc aactacccgc
    13081 tggatatatc tccggccacc gacgatcggc cgttcttctt caacctgatc ctgctcggcg
    13141 acctgtttga tccggcactc agcggcagcg gcgtctatcg caccagcatg gaagccatct
    13201 acatcctgtt cgccgtcatc ggcgtcacgg ccacactgag cgctctgttc attctcgtgc
    13261 cgctctggct gggagccaaa cggcgcaatc tggcgcgccc ctcagccatg acgctggcct
    13321 atttcggcgc gctgggcatg gccttcatga tggtcgagat tccgacgatg caaaaactga
    13381 ccgtctatct gggccagccg gtctattcgc tggcggtcgt cctgttctcc ttattgttgt
    13441 tcagcggcct gggaagctgg tggagcggcc gctgggcggc acagtccatt tcagccgaca
    13501 tgcgccgtgt cttcccggtg ttaatcggct tcctgattct ccacgctgtc ttcagcacct
    13561 tcgtcctgcg ccagacgctg caatttgatc tggccgggcg attggtggtt gccgtcctac
    13621 tcctttcggt catggggttc atgatgggca tccccttccc ggtgggtatc cgctgggtcg
    13681 gccgccgcca atcctccgtc gtcccctggc tgtggggcat caatggcgtc acctccgtgc
    13741 tcggctcggc cctggccacg gcgctatcga tccacatcgg ctttcgcctg acgctcgtcg
    13801 tggccactct ggtctacggc gcggcggcaa tgctcttcct gaatatcctg aaacaaacat
    13861 ccgaggagtc ggagtcaatc gatcctgttc tgccgagcgg gccgcacagc gccgatctcg
    13921 gaccggcctg attgagttcc gaatagagcg aatcgacaag acaagtacca cacaaggctg
    13981 gattaatata tgactaccct ccaacacaca catctctacc gcgaaatcca cgagcaaccg
    14041 aacgttgtac gccggctgct ggacaaagaa atggctaccg cgcaagcatt ggccgacctc
    14101 atccatggtc gcggcatcga ccatgtcgtc atcgccgccc gcggcaccag cgacaacgct
    14161 ggccgctatg ctcaatatgt ttggggagcc atgaacggcc tgtcggtcgg tctggcggct
    14221 cccagcctgt tctcgattta tcatcggccg ccgcgcttcg gcaacgcgct cgtgatcggc
    14281 atcagccaga gcggcaaaag tcccgacatc gtttccgtgc tggccgaggc gcgccgccag
    14341 ggagcgctga cggccgcgct gaccaatcgc cctacttctc cgctggccgg cgaggccgac
    14401 gtagtgatcg acctgcaggc aggggaagaa ctggccgtgg cagccaccaa gacctacacg
    14461 gccgagctgg cggccgtcgc cctgatcagc gccgccctca gcggcacggc tgaacaacga
    14521 gatgccctgc tgaccgtgcc ggaagccatg gaagcgacgc tggcgatgaa cgacgccgtc
    14581 gccgaaattg cgccccgtta ccggtacatg cagcggtgcg tggtcgtcgg ccgcggcttt
    14641 aatctcgcca cagccttcga gacggccctc aagctaaagg agatgaccta caccatcgtc
    14701 gaaccctaca gcagcgccga cttcctgcac gggccgctgg cgatgatcga acagggtttc
    14761 ccggtgatcc tcatcgcccc gtcgggcgtc atggcggccg agatgctcga cttcgcccgt
    14821 accctgcgtg agcggcaagc tgaactgatc gtcgtcagcg acgcggccga cctgctggat
    14881 acggcgcgag tccctctgaa actgcctgtg cccatgccgg aatggctctc gcccatcacg
    14941 agcatcctgc ccggccaaat gatggcgatg cacctggccc acacccgtga ctacaatatc
    15001 gacgcgccgc gcggcattat gaaggtgacg gaaacgcgat aagtgcgaat gacgaatcgc
    15061 tttgaaagag gctaaaccag ccgagcatgt tttctccagc gagaaaccga atagcggtcg
    15121 ttttcaattt catatcggtc ttttgtcgca ccgaataact atggtacact gccgttattg
    15181 tcgtctaggg atacctttag gcttccatca caggtatctc gccgcgtcct ttagttgcgc
    15241 tcaacctttt ggaatcatcc cgacccggtg attccattca taaaggttca ggcataacga
    15301 atgctgcgag aggcgacaaa ggaggtggac gagtcataag acagctaaaa caggattgct
    15361 ccaatggatc agtccctgac aagggcatat tatcgatcct ggccgacatg aatgatcgac
    15421 cgggacaaca tatttggagg agagttcgat atgtccaata aacgcttatg gcttcccatc
    15481 ttgttgatgg tactggccct gatgattgcc gcttgcggcg gcacagcccc gacagaaaca
    15541 accgcaccca ctgcagcacc ggcaccggcc gaagaagaag cggcgcccgc cgaagaagag
    15601 gccgcacccg ccgaaagtac cggagccaac cagcttgagt tctatagctg gtggacagcc
    15661 ggtggcgagg ccgacggcct gaacgccatg tacgatctct tcaaggccat gtaccccgac
    15721 atcgagatcg tcaatgcgac cgtggccggg ggcgccggat cgaacgcgaa ggctgtattg
    15781 gccactcgta tgcaggccgg cgatccgcct gattcgttcc aggtccatgc cgggcatgag
    15841 ctgatcgata cctgggtagt ggccgacaag atggaaccgg tcaccttcat cttcgaggaa
    15901 aacggctgga tggatgccta tccgcccgac gtcatctcca ttctctccta caatggcgag
    15961 atttggagtg ttccggtcaa tatccaccgc tcgaacgtca tgtggtacaa tcccgccgtc
    16021 ctcgaagcca atggcctgac tgccccgacg accattgaag agttcttcac tgtggctgac
    16081 gccctcaagg cagccggcgt cactccgttg gccctgggcg ataacggcat ctggacggcg
    16141 acccatctgc ttgagagtgt gctgctgggc acgctcggag cagacggcta tcgcggtctg
    16201 tggaccggcg ataccgattg gaacagccct gaggtgacga ccgcattgga atcattcgcc
    16261 cgcatgctcg actacgtcaa cgccgaccac gccgcgctgt cctgggatca ggctgcgcaa
    16321 ctggtcgccg atggcgatgc cgccatgacg atcatgggcg actgggccaa tggctatttc
    16381 acctctatcg accttaagcc gaacgtcgat tatggctacg ctccctcccc gagcacagac
    16441 ggcatcttca tgatgttgtc cgacacgttc agcctgccca agggcgcgcc acaccgcgac
    16501 aacgcgatca agtggctcac tctggccggc tcgaaggaag ggcaggatgc atttaatccg
    16561 ctgaaaggct ccatcccggc ccgcaccgac ggcgatcgct cgctgtacga tgagtatttg
    16621 caatcggcga tggatgactt cgccagcaac gccatcaccc ccagcctggc ccatggcgcg
    16681 gccgcgtccg agggttgggt cacggcgttc aacgacatca tgaccctgtt cgtcaccgac
    16741 cgcaacgtcg aaaacgcaca ggcggcaatg gcgaaggctt gtgtcgacgc cggtgtctgt
    16801 ggtcaataat cggtaattgg atcgacgagc gccgccgtgg gaaacccggc ggcgctcttg
    16861 ttcaatcggc attcgtcgcc ggcggcaatt tacaatgata gttgccgttt atccgttcaa
    16921 ttacaatccg acaatttcag gagtggcctt atgaaatccc agtcacgcaa cgagcgttgg
    16981 atcgccttcc tgatgatcct tccgtcgatc atcctgttgg gtatcttcgt ctacggtttc
    17041 atcgcctgga cgggagtcgt ctccgtgtcc aaatgggacg gcatccagcc cgattacacc
    17101 tttgtcggct tcaacaacta taaacaactc ttttcgctgg cgctgggtgg catatccgcc
    17161 atccgcttcc acatcgacct ctggaatact ttcttcttta ccattttctt tctcttcctt
    17221 tgccttattt ttgggttgtt gctggcaatt ctgctcgatc aaaagataaa gggcgaaggc
    17281 atttttcgaa cgatctattt gttccccatg gcgatctcct tcgtcgtcac cggcgtcgtg
    17341 tggcagtgga tcttcgcgcc ggggaccgaa tcaagggcgc gaggcgtcaa tgccttgctc
    17401 cagagccttg ggcttgataa cctgacctgg ggctggtaca ccgatccgac gcggatcggc
    17461 cctttccatg tcgctctcat cccggtgatc atcgccgccg cgtggcagat gatcggctat
    17521 acgatggcca tgtatctggc cgggctgcgc gccattcccg atgacctgcg cgaggccgcc
    17581 cgtgtggacg gagccggcag tttcggcgtc tatcgccacg tcgttctgcc gcttctcgcg
    17641 cccatcaccc tgagcgcgct catcattctg ggccatattt cgcttaagat tttcgacctc
    17701 atctacacca tgaccggcaa ggggccggcc ttcgccaccg acgtgcccgg tatcttcatg
    17761 ttcgagacga cctttcaggg caaccattat gcccaggggg cggcaatctc catcatcatg
    17821 ctcctgatgg tcgccctgat catcattccc tatctcatct acagcttccg tcaagaggtg
    17881 gaactatgac cgcacgtttc aatccggcgc gctttgccgt ctatctcttg ttgatcctgt
    17941 tcgctcttat ttttctgatc cccgtctacg tcctgttgag cagcagtttg aagacctttg
    18001 ccgaagtcca ggacttgtcg aggctatggg cgccaccgca agggctaaac ctggaaagct
    18061 tcaaggcggc atgggcaggc atccctgaaa aaggcctgcg tggtctggcc cccaacctga
    18121 tgaacagctt caagctgacg ataccggcga ccattctgtc ggccttgctg ggttcgatga
    18181 acggttacgt cctgtcgaag tggaagttcc ccgccgcgaa tatcgttttc ccgttgatgc
    18241 tgttcggcat gttcat
//
LOCUS       DAOMQG010000028        18112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1356_length_18112_cov_11.367614, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000028 DAOMQG010000000
VERSION     DAOMQG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 18112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18112
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1356_length_18112_cov_11.367614"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1016
                     /locus_tag="PLC06_03150"
     CDS             51..1016
                     /locus_tag="PLC06_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRO89473.1"
                     /translation="MSKRTVDFAGIRIGHVTHEAHYTGCTVFLCPDGTRGSVDARGPA
                     PGSRELALLAPDKPEDKDVDAILLTGGSAFGLAAADGVMQYLAERNIGHPTPIRPVPI
                     VPAAVVFDLFLNWGAFTPNAESGYSACAAADVFDGTIEEGNVGAGTGVLVGKWSGFEN
                     MMKGGFGVSSLKVGDVTVAAAAVVNAVGDVVDARSGVLAGARDPNGGWQADANELRYI
                     ERLAPLPPGTNTTLVVAATNATLSSSELARMTHQAHNGIAIAVRPSHTRHDGDVAFAL
                     STMQMVASLDLVNNMTVQVVTEAIRNGVRYCNTTLGVPGLRDWNV"
     gene            1035..1589
                     /locus_tag="PLC06_03155"
     CDS             1035..1589
                     /locus_tag="PLC06_03155"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRO89474.1"
                     /translation="MAQPVISVFGSSSPLPESEAYLEAERLGALLAEAGIAVATGGYG
                     GTMAAVSQGASEAGGRVIGVTCNHIEKWRPMPPNEWVAEEVRLESLRERLLHLVANNN
                     GMIALPGGIGTLSEVAFAWSLMQTGEIPRRPLVLLGPLWRETIRVYAQSEYIRPRDMD
                     LIYLATSAETAVGYVRQHLADQFG"
     gene            1613..2413
                     /locus_tag="PLC06_03160"
     CDS             1613..2413
                     /locus_tag="PLC06_03160"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HRO89475.1"
                     /translation="MPNDIIFLKLGGSLLTDKTGFEALRPEIMARLAEEIAAARRANP
                     SLKLVLGHGSGSFGHVAGAKYGTRSGVYGPEQWFGFAEVADAAARLNRIVVASLLSAG
                     VPAVGLSSFASARVSNGKITHLNVETLRSALNAGLVPVVYGDVAFDAIRGGTIISTEE
                     IMEYLSAEMPAAWLLLAGETPGVLDLLGEVIPLITRHTLPEVLPALGASRGTDVTGGM
                     ASKVLAMLDLVEARPNLSVRIFSGLEPGLLQALLMDPSLGSGTLLRYV"
     gene            2630..3148
                     /gene="hoxE"
                     /locus_tag="PLC06_03165"
     CDS             2630..3148
                     /gene="hoxE"
                     /locus_tag="PLC06_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxE"
                     /protein_id="HRO89476.1"
                     /translation="MTPRNEKLEPPSDDKRWRIVQATMQRNGFERDGLIETLHTVQES
                     FGFLDDDSLRYVAASLNAPLSQVYGVSTFYHLFSLKPAGRHSCVVCLGTACYIKGIPR
                     LLKEIENEYGIKAGQTTADGSLSLMTARCLGSCGLAPVVVFNGEIAGKINVEDALTRL
                     KTLLEVEDVRQA"
     gene            3132..4781
                     /locus_tag="PLC06_03170"
     CDS             3132..4781
                     /locus_tag="PLC06_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRO89477.1"
                     /translation="MFDKLDEITLAEQRHSESYIHTVNVCTAAGCMSSHSAAVKEALQ
                     EQARLAGGEHWCRVRGTGCMGLCAAGPLVTVDAEKPNEVMYQRVTVEDVAEIVGSLDT
                     EPVKRLRLHTDTPFFQQQKKIALENSGLIDPERIEEYIARDGYTGLLKAITEMSPSEV
                     IDEIVASGLRGRGGGGYPTGLKWTTVAKSDGETKYVICNADEGDPGAFMDRSVLESDP
                     HRILEGMAIAGYAIGASQGYVYVRGEYPLAVARLKVAIRQARRANMLGSGIGGTLFNF
                     HVDIRLGAGAFVCGEETALIASIEGKRGQPRPRPPYPAEFGLWGRPTLINNVETLANI
                     APIIRNGGQWYSQFGTSSSKGTKVFALAGSIRNTGLIEVPMGTPMRNIIYEIGGGIAG
                     VGDCKAVQTGGPSGGCIPAAYLDTPVDYESLSALGSIMGSGGMIVMDESASMVDVARY
                     FMEFCMTESCGRCVPCRVGTVHMYNVLDKFSRHEATLHDLELLEKLCDMVHSTSLCGL
                     GQTAPNPVLSTLHYFRDEYMDCIVPESNDGMLSSRKMEVAR"
     gene            4778..5503
                     /gene="hoxU"
                     /locus_tag="PLC06_03175"
     CDS             4778..5503
                     /gene="hoxU"
                     /locus_tag="PLC06_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HRO89478.1"
                     /translation="MKRPFKARIKTLKINGIDVGAREDETILELAQENGIFIPTLCHL
                     EGVHDVGSCRLCLVEVRGTNKLLPACMTYAEEAMEVITNSERLQTYRRQVLDLLFSER
                     NHVCSVCVSNGHCELQWMAEKLGISHVGVPYLYPQLAVDASHPYFSYDPNRCILCTRC
                     IRVCDEVEGAHTWDVMGRGVDCQIISDLNQPWGTADSCTRCGKCVQVCPTGALFQKGK
                     AVAEMEKRRDFVPYLQTMRGGRK"
     gene            5500..6036
                     /locus_tag="PLC06_03180"
     CDS             5500..6036
                     /locus_tag="PLC06_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRO89479.1"
                     /translation="MRNKPTLATVWLDGCSGCHMSFLDIDERILELAALADLVYSPLV
                     DTKEFPEMVDITLIEGAVSNEDDYSKIKMVRAHTKLLVSLGDCAVTANVPAMRNRFAA
                     KDVLARAYLENAGLNQHIPVQVIPELLAQSIPVHQVVPVDVFVPGCPPAADIIFYTLT
                     ELLAGRMPDLRSKTRFGA"
     gene            6054..7484
                     /locus_tag="PLC06_03185"
     CDS             6054..7484
                     /locus_tag="PLC06_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRO89480.1"
                     /translation="MSKTIIIDPVTRIEGHSKITIHLDDAGRVSDARFHVTQFRGFEK
                     LVEGRPFHEMPSLTARVCGICPVSHLIASAKACDALLAVKIPEAATNLRQVLNLAQIL
                     QSHALSFFHLSSPDLVLGMDAAPQTRNLFGVAADKPDLARDGIWLRQYGQEIIAHLAG
                     KRIHPAWVVPGGVSAPLTADRRDIILAKVPEAIQRIRRTLDWYKEVFGHFDAEIRTFA
                     NFPSLFLGLVSEQGELEMYDGKLRFVDATGRIVADGIEAHQFGDYVEEAVEPDSYLKS
                     PYYKSMGYPDGIYRVGPLARLNLIDRVGTPMAEGEWAEFRMLERGAALSSFQYHYARL
                     IEMLYCVEKIDKLLNWHDILSEHVRATADPNSLEGYGVSEAPRGTLMHHYKIDRKGLM
                     QWANLIIATGHNSLAMNKGVYQVARYFVHGEKIDEGMLNRVEAVIRTFDPCLSCSTHA
                     FGQMPMSIQLLSPDGELLDELKRGVR"
     gene            7563..8012
                     /locus_tag="PLC06_03190"
     CDS             7563..8012
                     /locus_tag="PLC06_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRO89481.1"
                     /translation="MTEMLVIGYGNLLRGDDGVGHHVVAALESLLPEGSAFAIHQLTP
                     EWSETISHARNVVFVDAAVGDSPGEVRCLRLVPRVYQPGSHELTAEGVLSMSADLFGR
                     YPNAHIVTITGKSFELSDSLTTPVAASVPVAARIVLELLGENKAPER"
     gene            complement(8053..9156)
                     /locus_tag="PLC06_03195"
     CDS             complement(8053..9156)
                     /locus_tag="PLC06_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRO89482.1"
                     /translation="MAKIFDRVAVESWGRDEIIQKFPREGLGDIKLGSQLIVNPGEVA
                     MFVRGGEAMGTFTPGRHTLTTENLPVLTDLFEAGLFGGKNVFTADVYFVKTTDLTLKW
                     GTVNPIIVEHPQRTPGASAIVGNGTYVTKVKDPWRFLNAMDAFRPSVRFQEVKNRLDP
                     MLAIMIADKMSELAIAKGLGPAQLQSFTKDMNELLIGLLQEEFDAIGMVLVDFAINMS
                     LHPDSLKVVTNMGYGTTYTQKQQADALMAAASNPSGGALSEVGFGAMGMAAMQQQQMQ
                     QQLFQQQMAAQQAAQSGQVPAAGTPPPAAAAPTAGAPAAMPDVMTPEQAAEFMQVGLQ
                     DVMAAIEAGDLKARKIGAAYRISKANLESFLSE"
     gene            complement(9179..9718)
                     /locus_tag="PLC06_03200"
     CDS             complement(9179..9718)
                     /locus_tag="PLC06_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89483.1"
                     /translation="MVEKVEGFYEKIVGDTGGLRNLLSKIPGLSGYMERGRRREADQL
                     LRDTITGRLEQARLSYSSVYQAVSRDIFMAMEYAERMGRADNLLMGLIGKIKDAPVGY
                     ASFFSAIKVDEEDLARLYSFDEQMLNHADQIQAEVDVLTKAVNEAGNLNEPLSALTTA
                     LLEANEIFNSRQEVLNGIK"
     gene            complement(9824..10339)
                     /locus_tag="PLC06_03205"
     CDS             complement(9824..10339)
                     /locus_tag="PLC06_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89484.1"
                     /translation="MGKTLGIILIIGGIVVGIIILALMSVYRSEGSLTSGAATLGIAL
                     GFLVLVLPQLGFGAFLLMRGRQESVVAESASKQRKMLGMIKAKGQVHISDLAIEMRST
                     REEIQGMLYELVNMGLYSGYVNWDEGILYSSEASELRTLDRCKNCNGQLEIAGKGVIR
                     CPYCGTEYFLP"
     gene            complement(10362..12293)
                     /locus_tag="PLC06_03210"
     CDS             complement(10362..12293)
                     /locus_tag="PLC06_03210"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRO89485.1"
                     /translation="MFKGKVIHNRLIALLALITLFLIFAPATRAQTKTFNWTNWDIDM
                     ALQPDGSLIVTETQTLNFQGEPFTFGFRSIPVGRRGNNDGISNVSVREGGQAFTESFS
                     NAPGTFEIVDNGDETRINWYFDPAIGEHTYTFSYTVHGAVRAGTMEEGSGDQIFWTVI
                     PSDHPARVDSSRVTITLPEGISPQRYFDTNEHLVEAYINDVVADNVLITVSEDERTIT
                     YVAQQPLMPGYSFDVRVQFPHGLLPAAMPEWQATEQRNDAIGLTVLALSCLLLIAGPL
                     AVVLLWYLRGRDPQLGIVVPDYITEPPDALPPAIVGSLVDEKVDMQDIMSSLVDLAHR
                     GYVLMEEDKKNSYTFTRTDKKDADLRGFESKFLNDIFRGESSRSLSSLQYKFAGKIPA
                     LRNMIIDDLIKEGYLPSNPQSVRLRYIWLAAGVFTLGIVSLFFLGILMEDNADLVCFP
                     VGAILLTGIALFIAARYMPRKTAKGTETAAKWMAFKKYLQNIKQYADIENSSDIFEKY
                     LAYAIAFGLERSFINSFSQSPATAAPPWFIPYPTGPMPMGGGYGMPRPSGGTSGGGTS
                     SGGGAPSLGDMSGGLTGGLAGMSAGLTRMLNNTSNVLKSTPPPANTGSSRSGGFSSGG
                     FSGGFSGGSSGGGGSAGFG"
     gene            complement(12376..13143)
                     /locus_tag="PLC06_03215"
     CDS             complement(12376..13143)
                     /locus_tag="PLC06_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89486.1"
                     /translation="MIFALGALGIPVLLSGCGRSDDSWERIQESGILRIGVDPTFPPF
                     AVTTGDTVEGIDIDLARALAAELGLEPQFSHFGYDGLYDALTTGQVDVLISALVIAPE
                     RTKEISYTTPYFDAGLYLFFPDSGRFAKGIGDMNGSTLAVELGSLGHVEALNRQKQDN
                     NLTVLPYESVDAAMAAAVEGEADAALVDYVSGRLFLRDNPAAAHLTFAPISGASEPYA
                     MAVRIGDESLLNELNAALTRLSTSGRLEAIIMKHLGQ"
     gene            complement(13173..14291)
                     /locus_tag="PLC06_03220"
     CDS             complement(13173..14291)
                     /locus_tag="PLC06_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRO89487.1"
                     /translation="MQPEKWWERLQQKAIFRYGWRFLGIAFLLALLQPSPTPITFGSP
                     QTVVTTAPILGVHTRLTDEVEEWKIQRTLQMVREMGASWIVEFFPWAYYQSEDGSVAW
                     EHPDLVIGHAKANGLKVIARLGYTPEWARPPDSPLTYLDRNAYDDFAAFAALFAERYK
                     DSVDYIIIGNEPNLSFEWGYRQTSPRDYVELLAAVYPAIKRADPKMIILAGALAPTIE
                     PEGSPWATNDLVYLQGMYDAGAADYFDGLAVHAYGLTFPADAEPGPEILNFRRIELVR
                     QVMVENGDEATPIYVTEMGWNDHPRWTMAVKPGQRIQYTIDALEYARENWPYVEALAI
                     WAFRYPAPTRSYPDYFTLVTPEFVTKPIYDALKAYSGN"
     gene            14455..15408
                     /locus_tag="PLC06_03225"
     CDS             14455..15408
                     /locus_tag="PLC06_03225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO89488.1"
                     /translation="MRVFITGATGFAGSHLVELLLKQGHQILALVHPATSHQDLPSHA
                     LVRPIAGDLLDPDGLARSVADARPDIIFHLAGQAYPARSWIEPAQTIAVNTGGTANLL
                     RAAAEYGRPRVVVVTSAEIYGPISANDLPLTESTTPHPRHPYGVSKLAAGQLTRAYWE
                     RYELPVVEARPFNHIGPHQAAGFVVPDFASQLAAIRLGRVEPVIRVGNLEPERDFTDV
                     RDVARAYWQLAEKGLPGEAYLICSGHSVSIRQLLDTLIELSGVEVQVETEQRRINPVD
                     TPCLYGSYAKIERDTGWRPQIPLRQSLADALADWDERLRRA"
     gene            15417..16196
                     /locus_tag="PLC06_03230"
     CDS             15417..16196
                     /locus_tag="PLC06_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRO89489.1"
                     /translation="MAKDKERLDVLLVERGLVETRARARAHIMAGEVTVDGVRVDKAG
                     TQISRSVQIELAAPMPYASRGGYKLAGALEQFGLDVADRVAADIGACTGGFTDVLLQR
                     GAARVYAIDVGQGQLVWKLRQDERVVVMERTNARYLDSLLEPVSLVVIDVSFISLKLI
                     LPAVRKWLSADADVVALIKPQFEAGPESVGKGGIVRDAAVRRAVLEDLLGWANEQDWS
                     VNGLIQSPIQGTDGNIEYLAWLRSTQQEGSDIAILIDSVLS"
     gene            complement(16211..16675)
                     /locus_tag="PLC06_03235"
     CDS             complement(16211..16675)
                     /locus_tag="PLC06_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRO89490.1"
                     /translation="MLDDNWIPPHSHDPNPVPPNNDPALMLHTGSEIVRLTPYELASF
                     PQFMLPHCYIVSTGHGTTGPFTFQGVRIIDLVTRYAKFGWTRVDIISGDGFATRLLRR
                     ALETMGDRPAILALMINGRPLRRDEGLVRLVVPDEKEDALQQVKWVSEICVH"
     gene            16783..>18112
                     /gene="lepA"
                     /locus_tag="PLC06_03240"
     CDS             16783..>18112
                     /gene="lepA"
                     /locus_tag="PLC06_03240"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRO89491.1"
                     /translation="MDQSKIRNFCIIAHIDHGKSTLADRLLQATGTISDREMQEQVLD
                     SMDIEREKGVTIKASAVRMNYTALDGETYEMNLIDTPGHVDFAYEVSRALQGCEGAIL
                     VVDATQGIEAQTLANLYMAVEADLELLPVVNKIDLPAAMPEDVAEEVEHITGIAAEDV
                     IPISAKTGVNIQDVLEAIVRKIPPPNGDRDGPLQALVFDSHYDSYKGVIAYVRVFEGT
                     LRSRQMIKMMSNDVVVEPIEIGIFNPGMMPVDELTAGEVGYIATGLKTVRDCRVGDTI
                     TLRDKPAAKPLHGYKSIKPMVYAGLYPSDGEDYPLLKEALERLQLNDASLVYEPETSN
                     ALNFGFRCGFLGLFHMEIVQERLEREYDLDLVATAPSVEYEVVMAGTGEVRFIESPAD
                     LPDESTIAEIREPWMRVQIFTPEEFYGTVMDLARKRRGEFIKQEHPAPGRV"
BASE COUNT         3742 a         5054 c         5312 g         4004 t
ORIGIN      
        1 ccgtgccttt ttgctggaaa aaggctgata gtcacattcc aatttgaact atgtcaaaaa
       61 gaactgttga ttttgccgga attcgtatcg gtcatgtcac tcatgaggct cattatacgg
      121 gctgcaccgt tttcctgtgt cctgacggga cgaggggaag cgtcgatgcg cgcgggcctg
      181 cgccgggtag ccgcgagttg gcgcttctgg cgcccgacaa acccgaagat aaggacgtcg
      241 atgcgatatt gctcaccggc ggcagcgcct ttgggttggc agcggccgat ggtgtgatgc
      301 aatatctggc cgaacgtaat atcggccacc cgacgcccat ccggccggtg ccgattgtgc
      361 ccgcggccgt cgtgttcgat ctgttcctga attggggcgc gttcaccccg aatgcagaga
      421 gcggctattc cgcttgtgcg gcggccgacg tgtttgacgg cacgatcgaa gagggaaatg
      481 tcggcgccgg cacaggcgtt ctggtgggca agtggtcggg cttcgagaat atgatgaagg
      541 gtggatttgg cgtgtcttcg ttgaaggtag gagatgtcac ggtggccgcc gcggcggtgg
      601 tgaatgcggt gggcgatgtt gtcgacgccc gatcgggcgt gttggccggg gcgcgagatc
      661 cgaatggcgg ctggcaggct gatgcgaacg agcttcgcta tatcgagcgc ctggcgcctt
      721 tgccgccagg caccaacaca acccttgtcg tggcggccac caatgcgacg ctatccagca
      781 gcgagcttgc ccgtatgact catcaggcac ataacggcat tgctatcgcc gtcaggccca
      841 gccacacgcg ccacgacggc gatgtcgctt tcgcgctctc gaccatgcag atggtcgcat
      901 cgctcgatct ggttaataat atgacagtcc aggttgtcac tgaggcaatc cggaacgggg
      961 ttcgttattg caacacaacg cttggcgtcc cgggcctgag ggattggaac gtctgataat
     1021 ggggaggtgt gcaaatggct caaccggtta tttctgtctt tggaagctct tcacctttgc
     1081 ctgagagcga agcgtatctt gaagctgaac ggcttggggc tttgctggcg gaggcgggca
     1141 ttgccgtagc taccggtggg tatggcggaa ccatggcggc cgtcagccag ggtgcctcgg
     1201 aggccggcgg gcgcgttatc ggcgtcacct gtaaccatat cgaaaagtgg cggcccatgc
     1261 cgccgaatga atgggtcgcg gaggaagtgc gtctggagtc tctccgggag agactgctcc
     1321 atctggtggc caataacaat ggcatgatcg ccttgccggg tggcatcggc actttgtccg
     1381 aggtggcatt tgcctggagc ctgatgcaga cgggcgagat ccctcgacgg ccgctcgtgt
     1441 tgcttggccc cctctggcgc gagacgattc gcgtctatgc ccagtccgag tatattcgcc
     1501 cgcgcgatat ggacttgatt tatctggcaa cttcggccga aacggccgta ggctatgttc
     1561 gccagcatct ggccgatcaa ttcggttaat caaccccttg atcaggtgac ctatgccgaa
     1621 tgacatcatt ttcctcaagc tgggcggatc actcctgacg gataaaacag gctttgaagc
     1681 gttgcggcct gaaatcatgg ctcgcctggc tgaggagatc gccgcggctc gtcgagcaaa
     1741 tccctcgcta aaattggtgt tgggtcatgg cagtggctcc tttggccatg ttgccggggc
     1801 aaaatatggc acccgatcgg gggtatatgg gccggagcaa tggttcggct ttgccgaggt
     1861 tgctgatgcg gccgcgcgcc tgaaccggat cgttgtcgct tcgttgttat cggccggagt
     1921 ccctgctgtc ggtctatctt ctttcgcctc ggccagagtc tcaaacggca agatcaccca
     1981 tctgaatgtc gaaacgctcc gttcggcgct caacgcgggc ctggtgccgg tggtctacgg
     2041 cgatgtagcc ttcgatgcaa ttcgtggcgg cacgattatt tccaccgaag agataatgga
     2101 atacctctcg gccgagatgc cggccgcctg gctgcttttg gccggtgaaa ctcccggcgt
     2161 gctcgatctg ctgggagaag tcatcccgct tataacccgt cacacgttgc ctgaagtctt
     2221 gcctgcgttg ggtgcttcgc gtggcacgga tgtcaccggt ggcatggcct cgaaagtcct
     2281 cgccatgctt gatctcgtcg aggcgcgtcc caacctctcc gtaagaattt tttccggcct
     2341 ggagccaggc ctgctccagg ctctattgat ggatccctcc ctcggttccg gcacattatt
     2401 gagatatgta tgacactcgt cacatctatt tgtgacaaat ttcactaatg taacccatcc
     2461 ttttctgcca caatatagac gactcccact atatcgtatt gttgcgggcg tcgtccccgt
     2521 tttgggctgg gcgtccgctc tgtagcttac aggcaatcct ggctcataag cccgtaatcc
     2581 ggcgattgcc ggtcggggag atatgggtcg tgagcgggaa aggagcgata tgaccccacg
     2641 gaacgaaaag ctggaacccc catcggacga taagcgctgg cgtatcgtcc aggccaccat
     2701 gcagcgaaac ggttttgagc gtgatggcct aatcgagacc ctccataccg tgcaggaatc
     2761 attcggattt ctggatgatg attcattacg ctatgtggcc gcgtccctga acgcgccatt
     2821 gagtcaggta tatggggtgt cgacatttta ccacctgttt tcattaaaac cggccggacg
     2881 ccattcctgc gtcgtttgcc tcggaacagc ctgttacatc aagggtattc cccgattatt
     2941 gaaagagatc gaaaacgagt acggcataaa ggcagggcag acaacggccg acggttcact
     3001 ctccctgatg acggcgcgtt gccttgggtc ctgcggcctg gcccctgtgg tcgttttcaa
     3061 tggtgaaatc gccggcaaga tcaatgtcga agacgccttg acgcgattga agaccctcct
     3121 ggaggtcgaa gatgttcgac aagcttgatg aaatcactct cgctgagcag cgacactccg
     3181 agagctatat ccataccgtg aatgtctgca ctgccgccgg atgtatgtct tcccatagcg
     3241 ccgctgttaa ggaagcgctc caggaacagg ccaggctcgc cggtggcgag cattggtgtc
     3301 gtgtccgcgg gacgggctgc atgggcctat gtgctgccgg gccgcttgtt acggtcgacg
     3361 ccgagaagcc caatgaagtc atgtaccaac gtgttacggt cgaggatgtg gcggagattg
     3421 tcggctctct ggatacagaa cctgttaagc gtttaagact tcataccgac accccgttct
     3481 tccaacagca aaagaagatc gcgctggaaa atagtggact gatagacccc gaacgaatag
     3541 aggaatatat cgctcgtgac gggtataccg gtttgctcaa agccatcacc gagatgtcgc
     3601 cgtcagaggt catcgatgaa atcgtggcca gtgggttgcg cggccgcggc ggcggcggct
     3661 atccgaccgg tctaaagtgg acgaccgtag ccaaatctga tggcgagacg aaatacgtaa
     3721 tctgcaacgc cgatgaaggc gatcccggcg cgtttatgga tcgcagcgtc ctggagagcg
     3781 acccgcaccg gattcttgaa ggcatggcca ttgccggcta tgccattggt gcctcccagg
     3841 gctacgtata tgtccgtggc gaatacccgc tggctgtcgc cagattaaaa gtggccatac
     3901 gccaggcccg acgcgccaat atgctcggca gcggcatcgg cggcacgctt ttcaatttcc
     3961 acgtcgatat ccgattggga gccggggcgt ttgtgtgtgg cgaggaaacg gctctcatcg
     4021 cttcaattga aggtaaacgc ggtcaacctc gcccgcggcc gccatatccg gcggaattcg
     4081 gtctctgggg gcggcctacc ctgatcaaca atgtggaaac gctcgccaat attgccccga
     4141 ttattcgcaa tggcgggcag tggtattccc aatttggaac cagcagcagc aaaggcacca
     4201 aggtgtttgc cctggccggc agcatcagga acacgggcct gatcgaggtg ccgatgggca
     4261 ctccaatgcg caatatcatt tatgagatcg gtggcggtat tgccggcgtc ggggactgca
     4321 aagcggtgca aacgggtggc ccatccggcg gctgcatccc ggcggcttat ctggacacgc
     4381 cggttgacta tgaatcgctc tccgcattgg gatccattat gggatccggt ggcatgatcg
     4441 tcatggacga atcggcgagc atggtcgatg tggcgcgtta cttcatggaa ttctgcatga
     4501 cggaatcttg cggccgttgc gttccttgtc gagttggtac cgttcacatg tacaacgtcc
     4561 tggacaagtt cagccgtcat gaggcgaccc tccatgacct ggaactgctc gaaaagttgt
     4621 gtgacatggt gcacagcacc agtttgtgcg gcctgggcca aacggctcca aacccggtgc
     4681 taagtaccct gcattacttc cgtgatgagt acatggattg cattgttccg gagtcgaacg
     4741 acggcatgtt gtcttctcga aagatggagg tggcccgatg aaacggccat tcaaagcgcg
     4801 gataaaaaca ctcaagatca acgggatcga cgtcggcgcg cgcgaggacg agacgatcct
     4861 ggagctggca caggaaaacg ggatattcat cccgacgctt tgccacctgg agggtgtcca
     4921 cgatgttggc tcctgtcgcc tgtgcctggt agaagtgcgt ggtaccaaca agctgctgcc
     4981 tgcttgcatg acctacgctg aagaggccat ggaagtcata accaattcgg agcgcctgca
     5041 aacctatcgc cggcaggtac tggacctgct gttctccgag cgcaatcacg tttgttcggt
     5101 gtgcgtgtcg aacgggcatt gtgagctgca atggatggcc gagaagctgg gcatcagcca
     5161 tgtaggcgtg ccctatctgt acccgcagtt agcggtcgat gcttcacatc catacttttc
     5221 ctatgacccc aatcggtgca tcctttgcac ccgatgcatc cgcgtctgcg atgaggtaga
     5281 aggcgcgcat acctgggacg tgatggggcg gggagtagat tgtcagatca tcagcgatct
     5341 gaaccagcca tgggggacgg ccgatagctg cacgcgctgc ggaaaatgtg ttcaggtttg
     5401 ccccaccggc gcgctgttcc aaaagggtaa ggccgttgcc gagatggaga agcgacgtga
     5461 ctttgtgccc taccttcaaa cgatgcgcgg aggtcgtaaa tgagaaacaa accaactctg
     5521 gccaccgtat ggctcgatgg ctgttcaggt tgccatatgt ccttcctgga tatcgatgaa
     5581 cgtatcctgg aattggcggc ccttgcggac ctcgtctaca gcccgctggt agacaccaaa
     5641 gagttccccg agatggtgga tattaccctt atcgaggggg cggtcagcaa cgaagatgac
     5701 tattccaaga taaaaatggt tcgtgcccac acgaagctgc ttgtatcgtt gggagattgc
     5761 gcggtgacgg caaatgtccc cgccatgcgc aatcgattcg ccgccaaaga tgtgttggcc
     5821 cgcgcctacc ttgagaatgc cgggttgaac cagcatattc ccgtgcaggt gattcccgaa
     5881 ttgttggcgc agtcgatacc cgtgcatcag gtcgtcccgg tcgatgtgtt tgttcccggt
     5941 tgtccgccgg cggccgatat cattttctac accctgacgg aattgctggc cggccgcatg
     6001 cccgacctgc gatccaagac ccgatttggc gcctgagcgt gaggaaccat gctatgagca
     6061 agaccattat tattgatccg gtgacccgta ttgaagggca cagcaagatc actatccacc
     6121 tcgacgatgc cggtcgagtc agcgacgcgc gttttcacgt cacccagttt cgcggcttcg
     6181 aaaagctggt cgagggcagg ccgtttcatg agatgccgtc tctgacggct cgtgtatgcg
     6241 gtatctgtcc ggtcagccat ttgatcgcct cagccaaggc ttgcgatgcg cttctggctg
     6301 tcaagattcc cgaggcggca acgaatttgc gccaggtgtt aaatctggcc cagatactcc
     6361 aatcccacgc cctgtcgttc ttccatttgt cgtcacccga cctggtgctg ggtatggatg
     6421 cggctccaca gacacgtaac ttgtttggcg tcgccgcgga caagccggac ctggcgcggg
     6481 acggcatctg gctgcgccag tatgggcaag agatcatcgc ccacctggcc ggcaagcgca
     6541 ttcatccggc ctgggtagtg cccggcggcg tcagcgcgcc actgacggcc gatcggcgcg
     6601 acatcatatt ggccaaggtg cctgaagcta tccagcgcat ccggcgcacg ctggattggt
     6661 acaaagaagt cttcggtcat tttgacgcag agattcgcac cttcgccaac ttcccatcgc
     6721 tgtttctggg cctggtttcc gaacaaggcg aactggagat gtacgacggc aagctgcgat
     6781 ttgtggacgc caccggccgc atcgtggccg acggcattga agcccaccaa ttcggtgatt
     6841 acgtcgaaga ggcggttgag ccggactcct atcttaaatc gccttactat aagtcgatgg
     6901 gctatccgga tgggatctat cgggtggggc cgctggcgcg tctcaacctt atcgaccgcg
     6961 tcggcacgcc gatggccgaa ggggaatggg ccgagttccg catgctggag cgcggagcgg
     7021 cgctcagctc tttccagtat cattacgctc gcctgatcga gatgctctac tgcgtcgaga
     7081 agatcgacaa actgctgaac tggcatgaca ttctcagcga gcacgtgcgg gccacggccg
     7141 atcccaacag tctggaaggg tacggcgtca gcgaggcccc gcgcggaacg ctgatgcacc
     7201 attacaagat cgatcgcaag ggattgatgc aatgggccaa cctgattatc gccaccggcc
     7261 ataatagcct ggccatgaac aagggcgtct atcaggtggc acgctacttt gttcacggag
     7321 agaagatcga tgaaggcatg ctcaatcggg tggaggcggt cattcgcact ttcgacccat
     7381 gtttgagctg ttccacccat gccttcggac agatgcccat gagcattcag cttctttcac
     7441 cggatggcga gttgctggat gagttgaagc gaggtgtgcg gtaagcagaa ccgacacctt
     7501 ctcatttgtt gccataaacg ggaagagacg catcacttgc gtctcttccc ccaatccttg
     7561 tcatgactga gatgctggta attggttacg ggaatctgtt gcgcggcgat gatggcgtcg
     7621 gccaccatgt ggttgccgcg cttgaatcgt tgctgccgga ggggtctgcc tttgccatcc
     7681 accaactgac gccggagtgg tcagagacga tcagtcacgc tcggaacgtc gtgtttgttg
     7741 atgccgcggt gggtgatagc cccggcgagg tgcggtgcct gcggttggtg ccgcgcgttt
     7801 accagcccgg ctcgcatgag ttaacggccg aaggcgtgct ctcgatgtca gcagatttgt
     7861 tcggccgcta tcccaatgcc cacattgtga cgatcaccgg gaagtcgttc gagctatcag
     7921 actccctgac cacgccggtg gctgcttccg tgcctgttgc tgcccggatc gtgcttgaat
     7981 tgctcggaga aaacaaagcg ccggaacgct gatggcgatt ccggcgcttg acaatttgat
     8041 cgcgctttcg agttattccg agaggaagga ttcgagattc gccttgctga ttcgataggc
     8101 cgcaccaatc ttcctggctt tcagatcgcc ggcctcgata gccgccatga catcctgcaa
     8161 gccgacctgc ataaactcgg ccgcttgctc cggggtcatc acatcaggca ttgccgccgg
     8221 tgctccggca gtcggtgctg cggccgccgg gggaggagtg cctgctgccg gcacctgacc
     8281 actctgtgct gcctgttggg ctgccatctg ctgctggaac aactgttgtt gcatctgttg
     8341 ctgctgcatg gcagccattc ccattgcgcc aaatcctact tcgctcaatg cgcctcctga
     8401 tgggttgctg gccgcagcca tgagcgcgtc ggcctgctgc ttctgggtgt aggtcgttcc
     8461 gtagcccata ttggtgacca ccttgaggga gtcggggtgg agcgacatat tgatggcaaa
     8521 atcgacgagc accatgccga tggcgtcgaa ctcttcctgg agcaagccaa tcagcaattc
     8581 gttcatatcc ttggtgaacg attgcaactg ggccgggcca agccccttgg cgatggccaa
     8641 ctcgctcatt ttgtcggcga tcataatcgc cagcattggg tcgagacggt tcttgacttc
     8701 ctggaagcga acactggggc ggaaggcgtc catggcattc aggaagcgcc acgggtcctt
     8761 cactttggtc acataggtgc cgttacccac aatggcactg gctcctggtg tgcgctgcgg
     8821 atgctcaacg atgatcggat tgaccgtccc ccacttgagg gtcaggtcgg ttgtcttgac
     8881 gaaatagaca tcggcggtga agacgttctt tccaccgaat aatcctgcct caaacaggtc
     8941 agtcagcacc ggcaaattct cggtggtcag cgtgtgacga cccggagtga aggtgcccat
     9001 ggcttcaccg ccgcgaacga acatggcaac ctcgccgggg ttcacgatca gctgtgagcc
     9061 gagcttgatg tctcccagtc cctcgcgagg aaacttctgg ataatctcat cccgccccca
     9121 gctctcgacg gctacgcgat cgaatatctt ggccataatt atgttctcct gatgcttctt
     9181 acttgatccc gttcagaact tcctggcggg agttgaaaat ctcgtttgct tccagaagcg
     9241 ccgtcgtgag cgccgagaga ggctcattca gattaccggc ttcgttgacg gccttggtca
     9301 atacgtcgac ctcggcctga atttgatcgg catgattcag catctgttca tcaaacgaat
     9361 agagccgggc caggtcttct tcatcgactt tgatggctga gaagaagctc gcgtatccga
     9421 ccggcgcatc cttgatcttg ccgatcaaac ccatcaacag attgtcggcg cgtcccatgc
     9481 gctcagcata ttccatcgcc atgaagatat cacggctaac ggcctgatag actgaactgt
     9541 aactcaggcg cgcctgctcc agccgcccgg tgatggtatc acgcaacagc tggtccgctt
     9601 cacgccgtcg gccacgttcc atgtacccgc tgaggcccgg gattttgctc agcagattgc
     9661 gcagcccgcc cgtgtcccca acgatcttct cgtaaaaccc ttctactttt tcgaccattt
     9721 ccagttaatc tccttcttga aaactttccc acatggacat atgagtccgg gacgggaaaa
     9781 tagcgaattt gccgttctga cctgaatcat aattccgtgt aatttatggc agaaagtact
     9841 cggtgccgca ataggggcag cgaattactc ccttgcccgc gatttccagc tgcccgttgc
     9901 aattcttgca tcgatccagc gtgcggagct cgctggcctc gcttgaataa agaatgccct
     9961 catcccagtt cacgtagccg ctatacagtc ccatgttgac caactcatag agcatgccct
    10021 gaatctcttc ccgtgtgctg cgcatctcaa tcgccaggtc ggatatatga acctggccct
    10081 tggccttgat catgccgagc atcttgcgct gcttgctcgc ggattcggcg accactgact
    10141 cctgccggcc gcgcatcagc aggaaagcgc cgaaacccag ttgcggcaga acaagcacca
    10201 gaaatccgag agcgatgccc aatgtggccg cgccggaagt cagactgcct tcactgcgat
    10261 agaccgacat caatgcgaga ataatgatgc cgacaacgat gccgccgatg atgaggatga
    10321 tcccaagcgt tttacccatt gaatacctct tgtcgctgcg cttatccgaa gcccgcgctg
    10381 ccgccgccgc cggacgaacc gccactgaag ccaccactga agccaccgct gctgaagccg
    10441 ccggagcggc tgctgcccgt gttggctgga ggtggcgtgc tcttcaggac gttggatgtg
    10501 ttgttcaaca tgcgggtgag gccggccgac atcccggcca gtccgccggt gagcccgccg
    10561 gacatatcac ccagactcgg cgctccgccc ccgctggaag tcccgcctcc gctagtcccg
    10621 ccgctcggcc gtggcatccc atatccgcct cccattggca tagggcctgt ggggtacgga
    10681 ataaaccatg ggggcgcggc cgtcgcgggt gattgcgaga acgaattgat gaaggagcgt
    10741 tccaggccga aagcgatggc ataagccagg tacttctcaa aaatatcgct ggaattctca
    10801 atatcggcgt actgtttgat gttttggaga tatttcttga aagccatcca cttggcggcc
    10861 gtttcagtcc ccttcgccgt ttttcgcggc atatagcggg cagcaataaa caaggctatt
    10921 ccggtgagaa ggatggctcc gaccgggaaa cagaccagat cggcattatc ttccattaag
    10981 atacccagga agaacaggct gacaattccc agggtaaaca cccctgcagc gagccatatg
    11041 taccttaaac gaaccgattg cggattactc ggcaggtagc cttccttgat gaggtcatcg
    11101 atgatcatgt ttcgaagtgc cgggatcttg cctgcgaact tgtattgcag gctgctgagc
    11161 gaccggctgc tctcgccacg gaaaatgtcg ttcaggaact tgctctcgaa gccccggaga
    11221 tcggcatcct ttttgtccgt gcgcgtgaag gtgtagctgt tctttttgtc ctcttccatg
    11281 agcacgtaac cgcgatgagc caggtcaaca aggctggaca tgatgtcctg catgtccacc
    11341 ttttcgtcga ccagcgagcc gactatagcc gggggcagtg catcgggtgg ctcagtaata
    11401 tagtccggca cgacaatgcc caactgagga tcgcgtccac gaaggtacca taaaaggact
    11461 acggcaagcg gcccagcgat aagcaacagg catgacaagg ccaaaaccgt aagccctatg
    11521 gcgtcgtttc gctgctcggt ggcctgccat tcaggcatag ctgccggcag caagccgtgg
    11581 gggaactgga cccgcacatc aaaactgtag cccggcatga gcggctgttg cgcgacatag
    11641 gtgatggtcc gctcatcctc gctgacggta atgagcacgt tatcagccac aacatcgttg
    11701 atataggctt caaccagatg ctcgttggtg tcgaaatatc tctgtgggga gatgccttcc
    11761 ggaagggtga tggtgacccg gctgctgtcg acccgggcgg gatgatcgct ggggatcact
    11821 gtccagaaga tctggtctcc tgagccttct tccattgtgc ccgccctgac agccccatgt
    11881 acggtatagc tgaaggtata ggtatgctcc ccaatggccg gatcgaagta ccagttaatc
    11941 cgggtttcgt caccattgtc tactatttca aaggtgccgg gcgcattcga gaatgattct
    12001 gtgaatgcct ggccgccttc gcgaacgctg acgttgctga taccgtcatt attgccccgg
    12061 cggccaaccg gtatgctgcg aaagccgaat gtgaaaggtt caccctgaaa attgagggtt
    12121 tgagtctcgg taacgatcag gctcccatcg ggctggagag ccatgtcgat gtcccaattc
    12181 gtccagttaa acgttttggt ttgcgcacgg gtggctggag caaaaatcag gaagagggtg
    12241 atgagagcaa gcagagcgat aagtcgatta tggataactt tgcccttgaa catggaaacc
    12301 gtccttgtgc tcaacgatgc tgaatggcag gaataattgt ccattataat tcaacttgag
    12361 agaaatgcag cagaatcatt gaccaagatg cttcataata atcgcttcaa ggcgacctga
    12421 tgtcgatagg cgagtcagcg cagcgtttaa ttcatttagc aaactttcat cccctatacg
    12481 tacagccatc gcataaggtt ctgatgcgcc cgaaattggg gcaaatgtca ggtgcgctgc
    12541 cgcgggatta tctctgagga acaggcggcc gctgacgtaa tctaccagtg ccgcgtcagc
    12601 ctcgccttcg acggccgccg ccattgccgc atcaacgctc tcatagggaa gaacggttag
    12661 attgttgtcc tgtttttgcc ggttcagcgc ttcaacatgg ccgagtgagc ctagctcgac
    12721 agcgagggtc gacccgttca tatcgccaat gcccttggca aatcggccgc tatccggaaa
    12781 gaacagataa agtccggcgt caaagtaggg tgttgtatag gagatttcct tggtgcgctc
    12841 gggcgcgatg accaatgcag aaatgagcac atcgacttgc ccggttgtca gggcatcgta
    12901 cagtccatca tacccgaagt gggagaactg tggttccagc cccagctccg cggccagggc
    12961 gcgcgccaaa tcgatatcaa ttccctcaac tgtgtcacct gtggtcactg cgaaaggagg
    13021 aaaagtagga tcgacgccta ttcgcaggat gcctgattcc tgaatgcgct cccacgaatc
    13081 atcggatcgg ccgcatcccg acaggagaac cggaattccc agggcgccga gcgcgaaaat
    13141 caaaggccat cttacgatcc tggataaagt catcagttac ctgaatacgc cttgagggcg
    13201 tcgtaaatgg gtttagtgac gaattcgggg gtcaccaatg tgaagtaatc agggtaggaa
    13261 cgggttggcg ccgggtagcg aaaggcccaa atggccaacg cttcgacgta gggccagttc
    13321 tcgcgagcat actccagcgc atcgatcgtg tactgaatgc gttgccccgg cttcaccgcc
    13381 atcgtccagc gcggatgatc gttccagccc atctcggtga cataaatcgg cgtcgcctcg
    13441 tcgccgtttt cgaccatgac ttgccgcacc agttcgatac ggcggaaatt cagaatctcc
    13501 gggccgggtt cggcgtcggc cgggaaggtc agcccatagg catgcactgc caggccatcg
    13561 aaatagtcgg ccgcgcccgc gtcgtacatc ccctgcagat aaactaaatc attcgttgcc
    13621 catggggaac cttccggctc aatggtcgga gccaatgccc ctgcgaggat gatcattttg
    13681 ggatcggccc gtttgattgc cgggtaaacg gcggccagta actcgacata atcccgagga
    13741 cttgtctgcc gataacccca ctcaaagctc agatttggct cgttgccgat gatgatgtaa
    13801 tccactgaat ccttatagcg ttcggcgaac aaggcggcga aggcggcgaa atcgtcgtag
    13861 gcgtttcggt ccagataggt gaggggcgaa tccggtgggc gtgcccactc cggcgtgtaa
    13921 cccagtcggg cgatgacctt caacccattc gccttcgcgt ggccgataac gaggtccggg
    13981 tgttcccacg ccacgctgcc gtcttcggac tgatagtaag cccaggggaa gaattcaacg
    14041 atccacgacg cgcccatctc tctgaccatc tgcaaggttc gctgaatctt ccactcctcg
    14101 acctcatccg tcaatcgcgt gtggacgccg agaatgggag cagtggtgac cacggtctga
    14161 ggcgacccga aggtgatcgg agttggagat ggttgcagca gggccagcaa gaatgcgatg
    14221 ccgaggaatc gccagccata acgaaagatc gccttttgtt ggagtctctc ccaccatttt
    14281 tcgggttgca tgacaaaacc cattatagga atttggtcgt tgtgggctat aaatgcctac
    14341 ctgcagttca ttaataggta ttcgaccgtt gagggctata ataagaaacg cgccgcggct
    14401 cagatgttgg tcagggggtg cggtggtcta tcagttcgcg gagtgatcga aattatgcgt
    14461 gtatttatta cgggagcgac gggattcgcg ggttcacatc tggttgaact cctgcttaag
    14521 caaggccacc agattttggc tctggtccat ccggcaacga gccaccagga tctgccgtcg
    14581 catgctctcg tccggccgat cgccggtgat ttgctggatc ccgacggttt ggcccggtcg
    14641 gtggcggatg ctcgaccgga tatcatcttc catctggcgg gacaggctta cccggcgcga
    14701 tcctggatcg agccggcaca gacgatcgcc gtcaatacgg gcggtacggc gaacctgctc
    14761 cgtgccgcgg ccgaatatgg acggccgcgc gtcgtggtgg ttacgagcgc cgagatctac
    14821 gggccgattt ctgcaaatga tcttcctctg acggaaagca caacgcccca tccccggcac
    14881 ccctatggcg tgagcaaact ggctgcgggg cagcttacaa gggcttactg ggagcggtac
    14941 gagttgccgg tagtcgaagc gcgccccttc aatcatatcg gcccccatca ggcggccggc
    15001 tttgtggtgc ccgatttcgc ctcgcaactc gcggctattc gactcggccg cgtcgaaccc
    15061 gtgatccgcg tcggcaatct ggagcctgaa cgggatttta cggacgttcg cgacgtggcc
    15121 cgtgcctatt ggcagctcgc cgagaagggg ctgcccggcg aagcctacct catctgttcc
    15181 ggccattcgg tgagcattcg tcaactcctc gacaccctga tcgagttaag cggagttgag
    15241 gtgcaagtag aaacagagca acgacggatc aatccggttg ataccccgtg cttgtatggc
    15301 agctacgcca aaattgagcg cgacacaggc tggcggccgc agatcccgtt gcgccaatcg
    15361 ctggccgatg cgctggcgga ttgggacgag cgcttgcgcc gcgcctgaga gccgctatgg
    15421 ccaaggataa ggaaagactc gacgttcttc tggtcgagcg tggattggtt gagacgcgcg
    15481 ctcgggcacg tgcccatatc atggccggcg aggtcactgt tgacggtgtg cgcgtcgata
    15541 aagccggaac tcagatttcg cgtagcgtac agattgagct tgctgcaccc atgccctacg
    15601 ccagtcgagg ggggtataag ctggccggcg cgctggaaca gttcgggctt gatgtggccg
    15661 atcgggtcgc ggccgatatc ggcgcgtgta ccggcggctt caccgacgtc ctgttgcaac
    15721 gtggcgcggc ccgtgtctat gccatcgacg tggggcaggg gcagctggtc tggaagctcc
    15781 gccaggacga aagagtcgtc gtcatggaac gaaccaacgc tcgctatctg gattcgctgc
    15841 ttgagccggt tagtctggtg gtcatcgatg tttcgttcat ttcgctcaag ttgatccttc
    15901 cggctgtcag aaaatggttg agcgctgacg ccgatgttgt cgccctgatt aagccccagt
    15961 tcgaggccgg gccggaatcg gtcggcaaag ggggaattgt ccgggatgcg gccgttcgtc
    16021 gtgccgtgct ggaagatctc ctcggctggg cgaacgaaca agactggagc gttaacggcc
    16081 tcattcagtc gcccattcag ggaaccgacg gcaatatcga atatctggcc tggcttcgat
    16141 ccacgcagca ggagggcagt gatattgcta tcctgatcga cagcgtcttg tcctgagcta
    16201 atctcgccgg ttagtgcaca catatttcgc tgacccattt cacctgctgt agcgcatcct
    16261 ctttttcgtc gggcacgacc agccgaacca ggccctcatc ccgccttagg gggcggccgt
    16321 tgatcatcaa cgccagtatt gccggcctgt cccccatcgt ttccaaggcc ctgcgcaaga
    16381 gacgcgtcgc aaaaccgtca ccactgatga tatcgacgcg cgtccaccca aatttcgcgt
    16441 agcgagtgac caggtctatt atccgcaccc cctgaaacgt aaaagggcca gttgttccgt
    16501 ggccggtgct gacgatatag cagtggggga gcatgaattg cggaaaactc gccaattcgt
    16561 agggcgtcaa tcgaacgatt tccgaacctg tatggagcat caatgcggga tcgttgttgg
    16621 gtggaacagg gttgggatcg tgtgaatggg gtgggatcca attgtcgtcg agcatttgat
    16681 cagttcctaa ttaatgccaa atatcccatg ggtaatagtc gggtaacaat ttcatctcct
    16741 ggcgcatggg taagcgcaga ccattttgat atacttggcc ctatggatca aagcaaaatt
    16801 cgaaattttt gcatcatcgc tcatatcgac cacggcaagt caaccttggc tgaccggctc
    16861 ttgcaagcta ccggcacgat ctccgatcgc gaaatgcagg agcaagtgct cgacagcatg
    16921 gatattgagc gcgaaaaggg cgtcaccatc aaggcctcgg ccgttcgcat gaactatacc
    16981 gccctggatg gcgaaacgta cgagatgaac cttatcgaca cccccgggca tgtggacttt
    17041 gcctatgagg tcagccgtgc cctgcagggc tgcgagggcg ctatcctcgt cgttgatgcc
    17101 actcagggga tcgaagcaca aacattggcc aacctctaca tggcggtgga agccgatctg
    17161 gagcttctgc cggttgtgaa caagatcgat ctccccgcgg ccatgcctga ggatgtggcc
    17221 gaagaggtcg agcatatcac cggcatcgct gccgaagacg tgatcccgat cagcgccaag
    17281 acaggcgtca acatccagga cgtgttggaa gcgatcgtcc gcaaaatccc cccgcccaac
    17341 ggcgaccggg atggtccgct gcaagcgctg gtattcgatt cgcattacga ttcctataag
    17401 ggcgtgatcg cctatgtgcg cgttttcgag gggacgctcc gcagtcgtca gatgatcaaa
    17461 atgatgtcga atgacgtggt tgttgaaccg attgagatcg gcatctttaa ccccggtatg
    17521 atgccggtgg atgagttgac ggccggagag gtcggatata tcgcgaccgg cctgaagacg
    17581 gttcgtgatt gccgggtcgg cgataccatc accctgcgcg acaagccggc ggcgaaaccg
    17641 ctccacggct ataaatccat caaaccgatg gtttatgcgg gactttatcc gagcgacggc
    17701 gaggattatc cgctgctgaa ggaagccctt gagcgcctgc aactgaacga tgcctcactg
    17761 gtatatgagc cggaaacatc caacgcgctc aacttcggct ttcgttgtgg cttcctgggc
    17821 cttttccata tggagatcgt ccaggaacga ctggagcgag agtatgatct tgacctggtg
    17881 gcgaccgccc ccagcgtcga gtatgaagtc gtcatggccg gcacgggcga ggtgcgtttt
    17941 atcgaaagtc cggccgattt gcccgacgag agcaccatcg ccgagattcg cgagccgtgg
    18001 atgcgcgtcc agattttcac ccccgaagag ttctatggaa cggtcatgga tttggcgcgc
    18061 aagcgacggg gtgaatttat caagcaggag catcctgcgc cgggtcgcgt tg
//
LOCUS       DAOMQG010000029        17577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1422_length_17577_cov_10.914450, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000029 DAOMQG010000000
VERSION     DAOMQG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 17577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17577
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_17577_cov_10.914450"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLC06_03245"
     CDS             complement(<1..1018)
                     /locus_tag="PLC06_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89492.1"
                     /translation="MGEPKGRKSLVAPSATIWGYIRHELLYLGFALMEIALLTPVVLV
                     ILGWARYWPPYQVGLWLLLIMLVPFNLIRLMSLLRLSLKQQRQILLIALAITIFQSWR
                     LLLYNAGPPWDLGWMREFFNSLGESGNLLWTRDLSIFIVTAFVWWRGIRLAIRHPEIN
                     NVGLRLRLGGLIFLPLILWFSNTLINTGVNVAASVLLFFFFALMVISLVRAENIEQEQ
                     SGTASTLNARWFMTVALAALLIIVLTGALTALITGESLFVVVAWLSPLWAALQFGATV
                     VGIILFEFAYPPLELLAGLVQWLSGILNILFGQVSATLLESPLFSDLDSPLIPTPTET
                     AEAIG"
     gene            complement(1039..2517)
                     /locus_tag="PLC06_03250"
     CDS             complement(1039..2517)
                     /locus_tag="PLC06_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRO89493.1"
                     /translation="MSESFHDLELVARDTRRETSRRARQSLMLAAISGDDFDMADIAH
                     ARGLVLRERENSVFSDVWVPLAFLFVIIGIVGNRNPGFIALGICLLIIVGVSTVWKNV
                     SLWGVSYRRHFDRTRVFPGEAVKMEVVIQNDKRLPLTWLRFRDQLPVPPDTNEHPLSL
                     SGELAGNFVLQSVYSMQSKERVNRSATLRFPVRGFYRIGPVTYESGDIFTLFTREREH
                     KYIDTLIVYPQIWPLEELGLPPKEPFGEVKVHHSLFTDPIRTRGIRDYHPQDRFRDVH
                     WKASARRGHLQTKVYDPSTGMTMAVFLNVSTFARHWMGFDPELLERAISVAASICNYG
                     VQQGWGVGLFANGAVPNSDQPIRVQPGRSPAQLGNVLEALAAVTEFATGSIDMMLLRE
                     STQLPWVSTIVLISAVVTEEILIALIRLKEAGRRVVLLALGDDPPPQLQIPGHMEQIS
                     IFHIPSSAPAFQAGHRSATATEAALSSIPTPEPVQLNFEVVE"
     gene            complement(2536..3474)
                     /locus_tag="PLC06_03255"
     CDS             complement(2536..3474)
                     /locus_tag="PLC06_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRO89494.1"
                     /translation="MIAESAARLRENIQKVIVGKANIIDLALIAILCEGHLLLEDVPG
                     TGKTTLTKTIAASLGCTFRRVQFTPDLLPSDLTGIYYYNQKAQEFEYRPGPVMAQILL
                     ADEINRATPRTQSALLEAMQERQVTVDIATHALPRPFLVMATQNPVELEGTFPLPEAQ
                     LDRFLMKVALGYPDEASENEMLLRFERSDPLDTLNSVVGPDDILSMQQETRTVRVENS
                     VRQYIVNICRATRAHADVMLGASPRATMALYRTCQARAAINGRDFVIPDDVKEMAPPV
                     LTHRLVVNPQTRLRGRVPEDVVREIVDTVPVPVEGN"
     gene            complement(3502..4932)
                     /locus_tag="PLC06_03260"
     CDS             complement(3502..4932)
                     /locus_tag="PLC06_03260"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HRO89495.1"
                     /translation="MSATQTNDQNSAKPIKFHKDVLTTIGNTPLVRLNSVTSGLEPLI
                     LVKAEFFNPGGSVKDRIGWPIIEDAERDGRLRLGGTIVEATSGNTGVGLAIAAAIKGY
                     RCIFVMPDKMSQEKILLLRAYGARVVVTPTAVEPEDPRSYYSVARRLVEETPNSILAN
                     QYYNPINPQAHYESTGPEIWEQTAGRITHFVAGMGTGGTIAGTARYLKEQNPEIQIVG
                     VDPIGSILYELHRSGQYTTAEGYKVEGIGEDFLPGTTDLSVIDEVVQVNDRESLLMTR
                     RLVREEGIFGGGSGGAAVVGAIKYVQKHRLGPEAVVVVILPDSGSRYLSKVFNDDWMR
                     ENGFLDQVWSDIRAGDIQSKKDSGQIYTAGSKDRLLDVVTLMKEYNISQLPVVNAEER
                     LVGLVSEIDLLNHLLKTDHVHQANETIEGMINTDVPVVGQNARLETLMSLIKDRSAVV
                     IVDNEDRVKGILTKIDILDYLSSQLR"
     gene            5106..6518
                     /locus_tag="PLC06_03265"
     CDS             5106..6518
                     /locus_tag="PLC06_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO89496.1"
                     /translation="MKRSRWTATITVIVLLLVTVVAVSAATYIVQPGDTLWRISRQFG
                     TTVDSIVRANNIANPNFIYVGQVLDIPGVGTSVPATPAPPPPGPTPVPPQPPATQTHV
                     VQPGDTLSRIAARYGTTVQAIAQANNIVNVNLIFVGQVLTIPGSGPAPVPTTPPTGPA
                     TPVPPSGDAFELGGQTQTFANKQAMKDSGMTWVKFQHKWAPGDSADALAPLIQDAKSN
                     GFKVLISVTGRQTYPAPDSIDFAAFAQFMGALAAQGPDAIEVWNEQNIDFEWPAGQID
                     PASYVNNMLKPAYEAIKAANPAVGVISGAPAPTGFDNTTNAWADNRYIAGMRAAGAAN
                     YANCIGAHHNAGATSPSATTGHPAGSHYSWYFLPTLDLYYNTFGGTRMVCFTELGYLS
                     GEGFSALPPNFSWASGTTVQEQAQWLREARDLSAASGKVRMMIVFNVDFTFFDPAGDP
                     QAGFAMIRPDGSCPACEAFK"
     gene            6635..7069
                     /locus_tag="PLC06_03270"
     CDS             6635..7069
                     /locus_tag="PLC06_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO89497.1"
                     /translation="MKVRNILATKKGQLITIRSDEPVRRAIALLVENRIGVLLVVDET
                     GKLVGILSERDVVRTAAYDEALFGRPVAEIMTKEVIVGMPQDDVLAVAHTMLEKRFRH
                     LPIMEDGELIGIISIGDVLKIQRDAYRGEVDTLETRIIAEGE"
     assembly_gap    7196..7295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7296..8629
                     /locus_tag="PLC06_03275"
     CDS             <7296..8629
                     /locus_tag="PLC06_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HRO89498.1"
                     /translation="FYSAERLFKETEMVVEVDMFDRLPTPYGLVRNGVAPDHQKIKSV
                     TAAFDRIANNPRFRFFGNVELGRDFSVDDLKDYYHQILYSTGAQTDRPLGIPGDELIG
                     SHPATEFVAWYNGHPDYRDHHFDLSQERVAVVGVGNVAIDVARILCRTREELMKTDIA
                     DYALEALVNSNVKEVYILGRRGAAQAAFTAPEAKELGELADCDALVPPAEAELDPLSQ
                     ATMANADRADVRKVEIIQELSRRQPSGKSKRLTLRFLVSPTELIDDGNGHVKQMKLVQ
                     NELYATDAGSLRPRATDRTETLDVGLVFRSIGYRGVPLPGVPFHESWGVIPNDGGRVL
                     DSATKEPVLGQYTAGWIKRGPTGVIGTNKPDAAETVENMLTDAREGRTLSPTHAMAEA
                     VTQLLDDRQVRPVSFADWKRLDELELARGKEEGRPRVKFTRIEDMLDALRS"
     gene            8637..9065
                     /gene="rnhA"
                     /locus_tag="PLC06_03280"
                     /pseudo
     CDS             8637..9065
                     /gene="rnhA"
                     /locus_tag="PLC06_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
     gene            9111..9506
                     /locus_tag="PLC06_03285"
     CDS             9111..9506
                     /locus_tag="PLC06_03285"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HRO89499.1"
                     /translation="MRVSCKGNPGPGTWAIVLESGDEWETLTGVEASTTNNRMELTAA
                     IQGLQLLGAGSQVQIFTTSDYLYQGATQWVRGWQSRAWQKKDGQPVANADLWLALVAA
                     SARYTVEWVNAKGQHLEGLERAGRLLSVA"
     gene            9556..9639
                     /locus_tag="PLC06_03290"
     tRNA            9556..9639
                     /locus_tag="PLC06_03290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9590..9592,aa:Leu,seq:cag)
     gene            9785..11092
                     /locus_tag="PLC06_03295"
     CDS             9785..11092
                     /locus_tag="PLC06_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89500.1"
                     /translation="MAIMLVVSVVFLVFLAGMLLAFLFTGLDRTVTTTQANMATEERA
                     YNPGVTLGHRVKPGADYEEQLKQARLEAAKKAAATPRGANSQIGRDGKSTLATAGKSA
                     DIDAMTAVRVARFHGWDGVRTGAPSVNALAAAGTAPAAVATAPAAATVAAPAVPPPTL
                     IAITDSMSPEEVRKARIANAKAESAYNKALKAASSGAPVAVAGAAPAAAAQAAAPMAA
                     APAVSHIEKPKLIEITPDMAPEDVRKARVANAKAEAAYNKALKAAGATPGAAQPAVAA
                     AAPVAPQPAPAASAAPAPAMAGIQPPQLIEITDGMAPEEIRRARVANAKAESAYNKAL
                     KAAGIDPAAVKAGAATVATAPAAPVQPVVAEAPAAAAPAPAPSAPAGGSQIPAGIERP
                     KLVEITADMSPEDIRRARVENAKAQSAFNKALKAAGIDPASVK"
     gene            11175..11918
                     /locus_tag="PLC06_03300"
     CDS             11175..11918
                     /locus_tag="PLC06_03300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRO89501.1"
                     /translation="MGLRRIARITFALIFLLLALALGWIFFNFGGLQDQRTKTFFQWA
                     TGDETERAPLVTVQRDACLGAPFVLPTDGFIGLLYADPNGPYSPGSPHQGIDIFSNAE
                     PGVMPVYAAYDGFIYRESDWRSALIQRVPDDPLQPGRQIWLYYAHMANRAGDSFIEPA
                     FPPGTDGVFVEQGTLLGYTGDYNGQSLRDIWVHLHFSIVLDNGRGRYANELEFANTLD
                     PSPYLGLPVNYACRSEAMTCRTNLDCAAN"
     gene            complement(12051..13415)
                     /gene="serS"
                     /locus_tag="PLC06_03305"
     CDS             complement(12051..13415)
                     /gene="serS"
                     /locus_tag="PLC06_03305"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRO89502.1"
                     /translation="MIDINLIREKPEWVKEQIARLGDDKAPIDAIIAADAHRREIIQE
                     VEELRRQRNEGSKQVGIWMGSMKKLEGELKRAGEAEQSTEAIRAQVADLQKNTEDAKE
                     DTRRLGDRIAHLEEELRAVEANVDEMMLWVPNLPHPSVPYGPDDSHNIAYPPEGVPLP
                     TFTGFEPKAHWDLGPELGLIDFDRGVKLSGSRFYVLKGAGARLQRAVIQFMLDFHVNN
                     HGYTEIYPPFMVRSAMFVGAGQLPKFADNIYRDAEEDYMWLGTAEIALTNLHRDEILD
                     EAELPLNYVAYTPCFRREKMSAGRDVRGIKRGHQFDKVEMYKFTTPETSYEHLDRLVK
                     HATDICEALELPYRRIEMVTGDLGFAAAKKYDVEIWAAGCKEWLEVSSASNCEAFQAR
                     RANVRYRPADGGRPRFAHTLNASGLALPRVMIALMENNQQADGSIRVPEALRSYMGGL
                     EYIS"
     misc_binding    complement(13446..13667)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13742..14662)
                     /locus_tag="PLC06_03310"
     CDS             complement(13742..14662)
                     /locus_tag="PLC06_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRO89503.1"
                     /translation="MHENLRFSPLIEQTLTNGGPVVALESTVITHGLPYPQNVETALG
                     MEEAVRSGGAIPATIAVIGGKVIVGINEDEVEYLGQLAGKGTVRKCSRRDLPIAIARK
                     EDGATTVAGTMIVAHMAGIEMFATGGIGGVHRGHPFDISADLRELGKTPVTVVSSGAK
                     SILDLPATREVLETEGVTIVGYGVDELPAFFARSCGLPVDVRLDTPEEVAALIRARRL
                     FGINGGTLITVPAPEEACMDEEEAEAAAARAAREADEAGIHGPSSTPWLLSRVVELTN
                     GRSMIANTALLQNNGRVAGQIAVALSRIKD"
     gene            14780..15727
                     /locus_tag="PLC06_03315"
     CDS             14780..15727
                     /locus_tag="PLC06_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89504.1"
                     /translation="MNSDAIVSANAEFERARWQAERERFAAWLLGRDSRLLPFDTIRR
                     NLRQQSSFYRGIQEVPLDLIIGSVGRYDEMTRAFLPLNEGLRQRWVKMAELAADEGWP
                     PVELYKVSNAYFVRDGNHRVSAARQLKFPTIEAHVWEYPADIEIGPKDSLDDVLNRFR
                     EQLFLEKTGLQLQHPEIDIHFTIGGRYPELQAQIEELRQKLEFIDEREVSFEEAADAW
                     YELVYLPSVQILRQSGLIEAFPGRTEADLFAWMSLHREKLRETYGDFENLSGLAQSLM
                     ETYKEKPATRMTRRVRSLFGGTELPRLEGLDDDATDEEE"
     gene            15851..17476
                     /locus_tag="PLC06_03320"
     CDS             15851..17476
                     /locus_tag="PLC06_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRO89505.1"
                     /translation="MNNSKKHEHGPIERPRFYSLLITVAALAFSLLSAGCGIDFNDPP
                     RLATATARSAAPPAGQAEPLILPAPTGTPGSAQAETDSSATEAIPAITPISEGDGLVL
                     WVDETSAEHKRVLDEIAAEFSDRYGTNMAVQLVSPAILPDLVSTGVLSGTLPDLILHP
                     IEYTTGWAESGILDAAATDEVADRLGRETFDSAALELVTVNGQIAALPSDGFHQLLLY
                     RADWFEELNLAVPDTYNAIAAAAERINNPEAIISGLVIPTESNLITTHQAFEQIALAN
                     GCRLIDENGEVLLLEPECAAALEQYYTTIHQFSPSGVQTDTSVAIAYLTGRTGIIMTS
                     PAILPDLAGLNEPAMPTCPECVTGEGGVNYLAQNTGIVTEIRGPNGEPTSFGNIRNLG
                     ITSTADRAAAQAFAEFWFNEGYERWLAVDSERKVPMRLGTQSEPRRFIDAWGILPLGE
                     SSLSLTDIYGAEVVERLRTGIAAAPRWGIREGYGGLMTRLYDDYVISIVLQEMLSGYF
                     DTTTTLREGYRRTIELIPNYVFPDRLAEETGEN"
BASE COUNT         3672 a         4918 c         5078 g         3809 t
ORIGIN      
        1 accctattgc ctcggccgtt tcagtggggg tggggattaa cggggaatcc aggtcgctga
       61 acaatggcga ttccagcaag gttgcggaga cttgcccaaa caggatattc agtatgccgc
      121 tcagccactg aactaatccg gcgagaagct ccaggggagg gtacgcaaac tcaaacagga
      181 ttatgccgac cacagtcgcc ccgaactgca gagcagccca gagcggactg agccaggcga
      241 cgactacaaa cagcgactcc ccggtgatca aggcggtcaa tgcacccgtc aagacgataa
      301 tcagcagggc tgcgagcgcc accgtcatga accagcgagc attaagcgtc gacgctgttc
      361 cactttgctc ctgttcgata ttttcggccc gcaccaggct gataaccatc agggcgaaaa
      421 agaagaacag gaggacagac gccgcgacat ttacgcccgt gttgatcagg gtgttgctga
      481 accacaggat cagcggcagg aagatcaatc cgccgaggcg caggcgcaag cccacattgt
      541 taatttccgg gtgacgaatt gccagcctga tcccgcgcca ccatacgaac gccgtgacga
      601 tgaagatcga caagtctcgc gtccacaaga gatttccact ttctcccaga ctgttgaaga
      661 actcgcgcat ccagcccagg tcccagggag ggccggcgtt ataaagcagt aatcgccagc
      721 tttgaaaaat ggtgatggct aaggcaatga gcaggatttg gcgctgttgt ttgaggctga
      781 ggcgcagcaa ggacatcagc ctgatcagat tgaacggcac cagcatgatg aggagcagcc
      841 acaatccgac ctgatacggc ggccaatacc gcgcccagcc caggattacc aggacgacag
      901 gggtaagtaa ggcgatctcc atcagcgcga aaccgaggta gagcagctcg tggcggatat
      961 agccccagat ggtagctgaa ggcgcgacca ggctcttgcg tccctttggt tcacccaagg
     1021 ggatgattcc cgccgatctc actctaccac ctcaaagttg agctgcaccg gttccggggt
     1081 tgggatgctg cttagcgctg cctctgtagc cgtcgcgctg cgatgtccgg cctgaaacgc
     1141 gggcgccgac gaggggatgt ggaagatgct gatctgctcc atgtggccgg ggatctggag
     1201 ctgtggcggt ggatcatctc ccagcgccaa caggacaacc cgccgcccgg cttctttcag
     1261 ccgtatcaga gcgatcagga tttcttcggt cacgacggct gaaatgagga cgatcgtgga
     1321 tacccagggc aactgcgtgc tctcgcgcag cagcatcatg tcgatggaac cggtggcgaa
     1381 ctctgtgacg gccgccagcg cttccagcac attgcccagc tgtgccggcg aacgaccagg
     1441 ctgaacgcgg attggctgat cggagttggg cacagctccg ttggcgaaaa gcccgacccc
     1501 ccaaccttgc tgaacgccat agttgcagat ggaagcggcg acgctgatcg cccgctccag
     1561 caattcagga tcaaagccca tccagtggcg agcaaatgtc gatacattca gaaagaccgc
     1621 catcgtcatc cccgttgatg ggtcataaac cttggtttgc aagtgacccc gccgcgcgct
     1681 cgctttccaa tgcacgtccc ggaagcgatc ctgagggtga taatcacgga tgcccctggt
     1741 tcgaatcggg tcggtgaaaa ggctatgatg caccttgacc tcaccgaacg gctccttggg
     1801 cggcaaccca agctcttcaa gaggccagat ttgcggatag acgatgagcg tatcgatata
     1861 tttgtgctct cgctcccgcg tgaataaggt gaagatatcg cccgattcat aggtcaccgg
     1921 gccgatgcga taaaagccgc ggacagggaa acgcagcgtt gccgagcgat tgactcgctc
     1981 cttgctttgc attgagtaga cgctctgcaa cacaaaattc ccggccaatt ctcctgacag
     2041 ggaaagcgga tgctcattgg tatccggtgg aacaggcagt tgatcgcgaa accgcaacca
     2101 ggtcagtggc aggcgcttgt cattctggat gaccacttcc attttgaccg cttcgccggg
     2161 gaaaactcga gtgcggtcga aatggcgccg atagctgaca ccccacagag acacgttttt
     2221 ccaaacggtg ctgacgccga cgatgatgag caggcagatg cccagagcga tgaatccggg
     2281 gttgcggttc ccaacgatgc cgatgatgac gaacaggaag gccagcggga cccagacgtc
     2341 actgaagacg ctgttttctc gctcgcgcag cacaagcccg cgcgcatggg ctatgtcggc
     2401 catgtcgaag tcgtccccgc tgattgccgc cagcatcaaa ctttggcggg cgcgtcggga
     2461 ggtctctctc cgggtatcgc gggcaacgag ttccagatcg tgaaagctct cggacatggc
     2521 ctgaggtttg cggaattagt ttccttcgac gggtacgggc acggtgtcga cgatctcgcg
     2581 gacgacatcc tcagggacgc ggccgcgcag ccgcgtttgc ggattcacca ccagccgatg
     2641 ggttagcacc ggcggcgcca tctctttgac gtcatcaggg atgacgaaat cgcggccgtt
     2701 gatggcggcg cgcgcctggc aggtgcgata gagagccatt gtcgcgcgcg ggctggctcc
     2761 cagcatgacg tcggcatgag cgcgcgtggc ccggcagatg ttgacgatgt attgccggac
     2821 ggagttctcc acgcgcacgg tgcgggtttc ctgctgcatg ctcaggatat catccggccc
     2881 caccacgcta ttcagagtgt cgagaggatc gcttcgctca aaccgcagca gcatctcgtt
     2941 ctcgctggcc tcatccggat agccgagagc gactttcatg agaaaccggt cgagttgggc
     3001 ttccggcaga ggaaatgtcc cttccagctc gacagggttt tgggtggcca taaccagaaa
     3061 aggccttggc agcgcatggg tggcaatatc gaccgtgacc tggcgctctt gcatcgcttc
     3121 caataaggcg gactgcgtgc gaggtgtggc tctgttgatc tcatcagcca acagaatctg
     3181 ggccatcacc gggccggggc gatactcgaa ctcctgcgct ttttgattgt agtagtagat
     3241 gcccgtcagg tctgagggca gcagatcggg ggtgaattgc acgcggcgaa atgtgcagcc
     3301 caaagatgca gcgatggttt tcgtcagtgt tgtcttgccg gtgcccggca cgtcttccag
     3361 cagaagatgc ccttcacaca ggatggcaat gagcgccaga tcgatgatat tggccttgcc
     3421 gacaattact ttctgaatat tttcgcgtaa ccgcgcggcc gattctgcaa tcatatgaac
     3481 tcttcctgac cgggaggtag gttaccgcaa ctgcgatgac aggtagtcca ggatatcgat
     3541 cttggtcagg atgcccttga cccgatcttc gttatcgacg atcacgacgg ccgagcgatc
     3601 cttgatgagc gacatcaggg tttccagccg cgcattctga cccacgaccg ggacgtcggt
     3661 gttgatcatc ccctcaatgg tctcgtttgc ctgatgaacg tggtcggtct tcagtaagtg
     3721 gttcagtaaa tcaatttcgc tgacaagccc aacaagtcgt tcttcggcat tgacgacggg
     3781 cagttgcgat atattgtact ccttcatgag cgtcacgacg tcgagcaggc gatccttcga
     3841 ccctgcggtg tagatttggc cactatcttt cttcgactgg atatctccgg cgcgtatgtc
     3901 gctccacacc tggtcgagaa agccgttctc gcgcatccag tcgtcattga ataccttgct
     3961 gaggtagcgg gatccggaat cgggcaggat gacgaccacc accgcctcag gcccaaggcg
     4021 gtgcttctgg acgtatttga tcgcgcccac gacggccgcc ccgcccgacc ccccgccgaa
     4081 aatgccttct tcgcgaacca gacggcgagt cattaacaag ctttcgcgat cattgacctg
     4141 gacgacctca tcgatcacgc tcaaatcagt tgtgcccggc agaaagtctt cgccgatccc
     4201 ttcaaccttg tacccctcgg ccgtcgtgta ctgacccgaa cgatgcaact catacaaaat
     4261 ggagccaatc ggatcgacgc cgacgatttg aatctccggg ttttgttcct tcagatagcg
     4321 ggctgtcccg gcgattgttc cgccggttcc catgccggcg acgaaatgag taatccgtcc
     4381 ggccgtctgc tcccagatct cggggccggt gctctcataa tgcgcctggg ggttgatggg
     4441 gttgtaatac tgattggcca ggatggagtt gggggtttct tcgacaagcc gccgggctac
     4501 ggagtagtag ctgcgagggt cctcaggctc aacggccgtg ggcgtgacga cgactctggc
     4561 cccataagcc cgcaataaca ggatcttttc ctgcgacatt ttgtcgggca tcacgaagat
     4621 acagcgatat cctttgatcg cggcggcgat ggccagacct acgcccgtgt tgccggaggt
     4681 cgcctcgacg attgttcccc cgaggcgcag gcgaccgtca cgttcggcat cttcgataat
     4741 cggccagccg atgcgatctt tcacggatcc gccgggattg aagaactcag ctttgaccag
     4801 tatgagtggc tcaagcccgc tcgtgacgga attgagccga actagcggtg tgttgccgat
     4861 agtcgtcagg acatccttat gaaacttgat gggctttgct gaattttggt cgtttgtttg
     4921 ggtagcgctc atagttttgg attccgtatc tgttcgccgg gaattcacgt gggtttcatg
     4981 caatgatata ggcaaatctg gcatgatgca tgtagcaaac ttatgtaaaa aagtcttgcg
     5041 gtcaaatcat gttatgtcta taatgtcaca agggataact tgacatatta ccaaggagag
     5101 tcgtcatgaa aaggtcgcgc tggacagcaa ccatcacggt cattgtcttg ctgcttgtca
     5161 cggttgtcgc cgtctcggcg gccacctata tcgtccaacc cggggacacg ctttggcgaa
     5221 tttcacgtca gttcggcacc acggttgatt ccatcgttcg ggccaataac atcgccaacc
     5281 ccaactttat ctatgtaggg caggtgttgg acattcccgg cgttggcact tccgtgcccg
     5341 caacgcctgc tccgccacct ccaggcccaa cccctgtacc cccacagccg ccggctacac
     5401 agacccatgt tgttcaaccc ggcgacaccc tgtcgaggat cgcggcgcgc tatggcacga
     5461 cggtgcaggc tattgcccag gcgaataata tcgttaatgt gaacctgatt ttcgttggcc
     5521 aggtgctgac gatacctggc agcggacctg ctcccgtgcc cacaactccg cccaccggcc
     5581 cggcgactcc cgtacctccg tcgggtgatg ccttcgaact cggtggccaa acgcaaacct
     5641 tcgccaataa acaggcgatg aaagattcgg gcatgacctg ggtgaagttc cagcacaagt
     5701 gggcgcccgg agattcggcc gatgctctgg cccccttgat ccaggacgcg aaaagcaatg
     5761 gcttcaaggt tttgatctcg gtgacgggcc ggcaaacgta cccggcgccg gatagcattg
     5821 attttgcggc gtttgcccag ttcatgggtg cgctggccgc ccaggggccg gatgccatcg
     5881 aagtatggaa tgagcaaaac attgattttg aatggcctgc cggtcagatc gatccggcgt
     5941 cgtatgtgaa caacatgcta aagccggctt atgaggccat taaggcggcc aaccctgcgg
     6001 tgggcgtgat ttccggagcg cccgcgccaa ccggctttga caacacgacc aacgcctggg
     6061 ccgataatcg ctacattgcc ggcatgcgcg cggccggcgc cgccaactac gccaactgca
     6121 tcggtgcaca ccataacgcg ggagctacat cgcccagcgc caccaccgga catccggccg
     6181 gttcgcatta cagctggtat ttcctgccga cccttgatct gtactacaac acgttcggtg
     6241 gcacccggat ggtttgcttt accgagcttg gttatctctc gggcgagggc ttcagcgctt
     6301 tgccgccgaa cttctcatgg gcatccggga cgactgtgca ggagcaggct caatggctgc
     6361 gtgaggctcg ggatctatcc gccgcaagcg gcaaggtgcg aatgatgatc gtcttcaatg
     6421 tcgacttcac cttcttcgac ccggcaggcg atccacaggc gggctttgcc atgattcgcc
     6481 ccgacggcag ttgtccggcg tgcgaggctt tcaagtaatt taaaagttgg gttcgagcaa
     6541 tttgaggcgc aaaggggcaa cctttgcgcc tttttgttat acttgcgtca gttcattagc
     6601 ggccgggagc cgctcttccc tggagaggat ccacatgaaa gtccgaaata ttctggcaac
     6661 caagaaaggt caactcatca caatccgctc tgatgagcct gttcgacggg ccatcgcctt
     6721 actggtcgaa aatcgtatcg gcgtgttgtt ggtcgtcgat gagacgggca aactcgtcgg
     6781 cattctcagc gagcgcgatg tcgtgcgcac tgcggcatat gacgaggctt tgttcggccg
     6841 ccctgtagcc gagatcatga ccaaggaagt cattgtcggc atgcctcagg atgatgtgct
     6901 cgccgtggca cacacgatgc tggagaaacg tttccggcat ttgccgatca tggaagatgg
     6961 cgagctgatc ggcatcatct cgattggcga cgttctgaag atacagcgag atgcctaccg
     7021 cggtgaagta gacaccttag agacccggat cattgcagaa ggagagtaat agtcccatgt
     7081 cccaaattgg aacaacggcg caacctttgc gggtcgccat tattggtgca ggcccggccg
     7141 gcttctattc ggccgagcgc ctgtttaagg aaaccgagat ggtcgtcgag gtcgannnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcttc tattcggccg agcgcctgtt
     7321 taaggaaacc gagatggtcg tcgaggtcga catgttcgat cgcctgccga caccctatgg
     7381 ccttgtgcgc aatggcgtgg cccccgatca tcagaagatt aaatccgtga cggccgcatt
     7441 tgaccgtatt gcgaacaatc cacgattccg tttctttggc aatgttgaac tcggacggga
     7501 tttcagcgtg gatgatttga aagattacta tcatcagatc ctctactcga ccggcgcgca
     7561 aaccgaccgg ccattaggca tccccggcga tgagttgatc ggcagccatc cggccacgga
     7621 gtttgtggcc tggtacaatg gccatcctga ctatcgcgat catcatttcg acctgtcgca
     7681 ggagcgtgtt gcggttgttg gtgtgggcaa cgtcgccatt gatgtggcgc gcattctttg
     7741 ccgcacacgc gaggaattga tgaagaccga catcgcggat tacgcgttgg aggcccttgt
     7801 taacagcaat gtgaaggaag tctacatttt gggacgacgt ggcgctgccc aggccgcctt
     7861 caccgcgcca gaggcaaaag agcttggcga gttggcggac tgcgatgcgc tggtgcctcc
     7921 ggcagaggcg gaactcgatc ctttgagcca ggcaaccatg gccaacgccg accgcgccga
     7981 tgtgcggaaa gtggagatca tccaggaact gtcgcgccgt caaccttcgg ggaaatccaa
     8041 acgcttgacc ctgcgttttc tggtatcgcc gactgagctg atcgacgatg ggaacgggca
     8101 tgtcaagcag atgaagctgg tgcaaaacga gctttacgcg actgatgccg ggtccttgcg
     8161 gccgcgcgcc actgatcgaa ccgagacact ggatgtaggt cttgtttttc gctccattgg
     8221 ttatcgcggc gttccattgc cgggtgtccc gttccatgaa agctggggtg ttatcccgaa
     8281 tgatggcggc cgcgtgctcg attcggcgac aaaagagcct gtcctggggc agtatacggc
     8341 cggatggatc aaacgcggcc caacgggggt tatcggcaca aacaaacccg acgcggccga
     8401 gacggtggag aatatgctca ccgatgcgcg cgagggccgt accctcagcc cgacccacgc
     8461 gatggccgaa gctgtgaccc aattgctcga cgaccgtcag gtacgccctg tgtccttcgc
     8521 cgattggaaa cgcctggatg agcttgaatt ggcccgcggc aaggaagagg ggcggccgcg
     8581 cgtcaagttc acgcgcatcg aggatatgct ggacgcgctt cgctcctgac aggcatatgc
     8641 ctgataaagt tgtgatcttc agtgatggag gggctgaccc aaaccctggc attggcgggt
     8701 gggcggccct tcttgcctat ggcgaccatg aaaaggtgct cacgggcaat gatcccaaaa
     8761 ccaccaataa tcgcatggaa ctgcaggcgg cgattagcgc gttgcgcgcc ttgaagcgtc
     8821 cttgccaaat cgaattccac acagattcgc aatatgtgca acggggcatc acggacggcg
     8881 tgaaaaaatg ggcggccgca gactggaaaa ccaaaaatgg ccgcccaatt cctaactcag
     8941 acctctggca ggaacttcgt gagctggtca agcgacacga aatcagctgg cattgggttc
     9001 gtgggcacag tggcgatgtc gccaatgagc gtgtcgacca attggccaga gaggcacgtt
     9061 tgcagataac gcccaggatc aatttgaagg cgggcgttcc gcgactgtat ttgcgtgttt
     9121 cctgcaaagg gaatccggga ccgggaacgt gggcgattgt actggaatcg ggcgatgaat
     9181 gggagacgct aaccggcgtg gaagcttcca cgacaaacaa tcgcatggag ctaactgcgg
     9241 ctatccaggg cctgcaactc ctcggtgcgg gtagccaggt gcaaattttc acgacttcgg
     9301 attacctgta ccagggcgcg acccagtggg tgcgtgggtg gcaatcgcgg gcgtggcaaa
     9361 agaaagacgg ccaacctgtg gcgaacgccg acctatggct ggctttggtt gccgcttccg
     9421 cgcgatatac cgttgaatgg gttaatgcca aggggcagca tttagagggc ctggaacggg
     9481 ccggcaggtt gttgtcggtt gcctgattat ggcattgaca attgaacccg ctctgataca
     9541 atacccatcc atcgagccga agtggcgaaa ctggcagacg cgctacgttc agggcgtagt
     9601 gagggttccc tcgtgtgggt tcgaatccca ccttcggcat gaagtggatt atgatgcacc
     9661 gacggcccga actgttcaag ttcgggccgt tttctgtttt gagataatgt tcacaaacgc
     9721 tataatgtcc ccaaatttca gggatgatat ataatatagc cgcctttaga tgaggggtat
     9781 ttttatggca ataatgctgg tcgttagtgt agttttcctt gttttcctcg cgggcatgtt
     9841 actggccttt ctgtttacag gactggatcg aacggtcact acgacccagg ccaatatggc
     9901 gactgaagag cgtgcctata accctggggt gacgctcggc catcgggtca agccaggggc
     9961 agattacgaa gaacaactca agcaggctcg actggaagcg gccaagaaag cggcagctac
    10021 tcctcgtggc gcgaatagcc aaatcggccg cgacggcaaa tcgaccctcg ccacagccgg
    10081 caagtcggcc gacatcgatg cgatgactgc cgtgcgggtt gcccggttcc atggatggga
    10141 cggcgtgcgg accggtgctc cgtccgttaa cgcgctcgcc gctgcgggta ctgcgccggc
    10201 tgccgttgcc acagcgccgg ctgccgctac ggtcgctgcc ccggccgttc ccccgccaac
    10261 tctaatcgca attacggaca gtatgtctcc ggaagaggtg cgcaaggctc gcatcgccaa
    10321 tgccaaggcg gaatccgcct acaacaaggc tttgaaggcc gcgagttcgg gcgctcccgt
    10381 cgctgtggcg ggcgctgctc cagccgctgc cgcgcaggcg gccgcaccga tggctgccgc
    10441 accggcagtg tcccatattg aaaagcccaa gctcatcgag attaccccgg atatggctcc
    10501 cgaagacgtg cgcaaggcgc gcgtagccaa tgcgaaggcc gaagccgcct ataacaaagc
    10561 gttgaaggcg gcaggtgcga ctcccggagc tgcgcaaccg gcagttgcgg ctgctgcgcc
    10621 ggtggctcct cagcccgctc cagcggcgtc tgcggctcct gctcccgcca tggccggtat
    10681 tcagccacct caactcatcg aaattaccga cggtatggct cctgaagaaa ttcgtcgggc
    10741 gcgagtggcg aacgccaagg ctgagtccgc ctacaacaaa gccctgaagg cggccgggat
    10801 cgaccccgcg gctgtgaagg cgggtgcggc gactgtcgcc acggctccgg ctgcccctgt
    10861 tcagccggtt gtcgccgaag ctccggcagc agcggcccct gcgcccgcgc catctgctcc
    10921 cgcgggtggt tcgcagattc cggcaggcat agagcggccg aagcttgttg agatcactgc
    10981 ggatatgtcg cctgaagaca ttcgtcgggc gcgtgtcgag aatgccaaag ctcaatcggc
    11041 cttcaataag gcgcttaaag cggcagggat cgacccggct tcagtgaaat aagctggaag
    11101 agggattgtc gaatttctaa tcccggctga ggaaataacc tcggccggga tttttcattc
    11161 atgcagtgag aaagatggga ttgcggagaa tcgcgcggat tacgtttgcc ttgatattcc
    11221 tgctgttggc attagctctg ggttggatat ttttcaattt tggagggcta caggatcaac
    11281 ggacgaagac gtttttccaa tgggccaccg gcgatgagac tgagcgcgca ccgctggtga
    11341 ctgtgcaacg tgatgcttgc ctcggagcgc cgtttgtctt gccgacggat gggttcattg
    11401 gcttgctcta tgccgaccca aacggcccgt attcccctgg atctccccat caggggatcg
    11461 acatctttag caacgcggaa ccgggcgtga tgcccgttta tgcggcctat gacgggttta
    11521 tctatcggga atccgattgg cgcagcgccc tgatccaacg cgtgccggat gatcctctcc
    11581 agcctggccg tcaaatctgg ctatactacg cccatatggc caatcgcgcc ggcgacagct
    11641 tcatcgaacc ggcattcccg ccgggcacag atggggtgtt cgttgaacag gggacgcttc
    11701 tcggctacac gggtgattac aacggtcagt cattgcgcga tatttgggtt catctccatt
    11761 tttccatcgt tttggataac ggtcgtggtc gctatgccaa tgaacttgaa ttcgccaata
    11821 cgcttgatcc gtcgccttat ctgggcttgc cggtgaatta tgcttgccgt tctgaagcca
    11881 tgacttgtcg gaccaatctg gattgtgccg caaactaatg cggcgggcac aatcatgcaa
    11941 cctcatccaa tagcggcttg tgcccaaact agcctgtatt aaaatgcccg tccgacaaac
    12001 tgaccggacg ggcatttaac atttcatgga gcgcaacgga ttggttaagc tcagcttatg
    12061 tattccaatc cacccatgta cgaacgtagc gcttccggca cgcggatcga accatctgcc
    12121 tgttggttgt tttccatcaa ggcgatcatt actctgggca atgccaggcc gctggcgttg
    12181 agcgtgtggg cgaagcgcgg ccgcccgccg tcggccgggc gatagcgaac attggcgcgg
    12241 cgggcctgga acgcttcgca attgctggcc gagctgactt ccagccactc cttgcacccc
    12301 gcagcccata tctcaacgtc gtactttttg gcagcggcaa agccgagatc tccggtcacc
    12361 atctctattc ggcggtaagg cagctccagg gcctcgcaaa tgtccgtggc gtgcttcacc
    12421 aaccgatcga gatgctcata gctcgtttcc ggggtcgtaa atttatacat ctccaccttg
    12481 tcgaactgat ggccgcgctt gatgccgcgc acatcccgac cggcgctcat cttctcgcgc
    12541 cgaaaacagg gcgtataggc gacgtagttg agcgggagtt cggcctcatc cagaatttca
    12601 tcacgatgaa ggtttgtcag ggcgatttcg gccgttccca gccacatata atcctcttcg
    12661 gcgtcgcgat aaatattatc ggcgaatttg ggcaactgac cggctccgac gaacatggcc
    12721 gagcgaacca tgaagggcgg gtaaatctcc gtgtagccgt gattgttcac gtggaagtcg
    12781 agcatgaact ggatcaccgc tcgctgcagg cgtgcgccgg ctcccttgag cacatagaag
    12841 cgcgagccgc tcagcttgac gcctcgatcg aaatcgatca agcccaattc aggacccagg
    12901 tcccaatgtg ccttcggctc aaaaccggtg aatgtcggta aaggtactcc ttcaggcgga
    12961 taagcgatat tgtggctgtc gtcaggccca taaggcaccg aagggtgggg taagttaggc
    13021 acccaaagca tcatctcatc gacgtttgct tctaccgctc tcagttcttc ttccaggtga
    13081 gcaatgcgat cacccaaccg gcgagtgtct tctttggcat cttctgtgtt tttctggaga
    13141 tcggcaactt gtgcgcggat ggcctcggtc gattgctcgg cttctccagc ccgcttcaac
    13201 tctccttcca gctttttcat cgagcccatc cagataccga cttgtttcga gccttcgttg
    13261 cgctgccggc gtagctcttc cacttcctgg atgatttccc tgcggtgtgc atcggcggct
    13321 atgatggcat cgatgggcgc tttgtcgtcg cccaatcggg cgatctgttc cttcacccac
    13381 tccggtttct cgcgaatcag atttatgtcg atcatgtcac tcctcaaata aaaagccccc
    13441 ttcgtttcca ggacgaaggg ggctcgtggt gccacctggg tttgccaatc cgcgaaggat
    13501 gggcctctca tgcgcgataa cggacgcatc cggctctcct taaacctttc ggcttccgaa
    13561 gagcaactcg ggaggggatt tctgatgctc gcggcattgg cttgcaccat acgccaactc
    13621 tcttgggctc acgaccgcgt cgtacttgtc tccgtcgatg ttgttaggct atttcaattg
    13681 gtgagattat aggtctgagg tatcagactg tcaataaccg tgttgcagcc aactcgaggc
    13741 cttaatcctt gatgcgtgat aatgcgacag cgatctggcc tgccacgcgg ccgttatttt
    13801 gcagcagggc tgtgttggcg atcatactgc ggccgtttgt aagttcgacg acccgactca
    13861 aaagccaggg cgtcgacgat gggccatgga tgcccgcttc gtccgcctcg cgcgcggccc
    13921 gagcggcggc cgcttcagct tcctcttcat ccatgcaggc ttcttcaggc gcggggacag
    13981 tgatcagtgt gccgccgttg atgccaaaca ggcgacgggc gcggatgagc gcagctacct
    14041 cttcgggcgt gtcaagacga acatcgaccg gcaagccaca agagcgagcg aagaaagcgg
    14101 gcaattcatc cacgccatag ccgacgattg taactccctc agtttccagc acttctcgcg
    14161 tagcgggcag atcgaggatg gacttggcgc cgctgctgac aaccgtcacc ggtgtcttgc
    14221 ccagttcgcg caggtcggcg ctgatatcga atgggtggcc acgatgaaca ccaccgatgc
    14281 cgcccgtcgc gaacatctcg atgcccgcca tatgagcgac gatcatcgtc cctgcgacgg
    14341 ttgttgcgcc atcttccttg cgggcgatgg cgatgggaag atcgcgacga ctgcacttgc
    14401 ggaccgtccc tttgcccgcc aattgaccca aatattcgac ctcatcctcg ttaatcccga
    14461 cgatcacctt gccgccgatg acggcgatag tcgccggaat tgcgccaccg gaacggacgg
    14521 cctcttccat tcccagagcc gtttccacgt tttgtggata gggcaaccca tgggtaataa
    14581 ccgtgctttc caaggcgaca accgggccgc cgttggttaa tgtctgctca attaaagggg
    14641 aaaatcgtag gttctcgtgc atggttgaga ttgtatcaca cccttggcca gcatcatgtc
    14701 caatatcggc gtgcttattt ttgtgaaata gtctgttgtc atgttgcggg atagtgacta
    14761 gactcaatcc cgaaagaaaa tgaacagtga tgcgattgtt agcgctaatg cggaatttga
    14821 gcgtgctcga tggcaggccg aacgggaacg cttcgcggcc tggttattgg gccgggactc
    14881 acgcctgctg ccatttgata ccatccgaag aaatctgcgg caacaaagct cgttttatcg
    14941 cggcatccag gaggtccctc tcgatctgat catcggcagc gttggacgat acgatgagat
    15001 gacccgcgct tttctgccgc tcaatgaggg cttgcggcag cgatgggtga agatggcgga
    15061 actggctgcc gacgagggct ggcccccggt tgagctgtac aaggtgagca atgcctactt
    15121 tgtgcgtgac gggaatcatc gcgtctcggc cgcacgccag ttaaagtttc cgacgattga
    15181 ggcccatgtc tgggaatacc cggccgatat cgagataggc ccgaaagaca gcctcgacga
    15241 tgtcctgaac cgctttcgcg agcaactgtt tctggagaag acaggcctgc aactccaaca
    15301 ccctgagatc gatatacact ttactattgg cggccgctat cccgagcttc aggcccaaat
    15361 tgaggaatta cggcagaagc tggagttcat cgacgagcga gaagtatcat ttgaagaggc
    15421 ggcagacgcg tggtatgaac tcgtttattt gcctagtgtg caaatcctga ggcaatccgg
    15481 cttgattgag gcgtttcccg gccgaaccga agccgatttg ttcgcctgga tgtcgctcca
    15541 tagagaaaag ctgcgcgaaa cctatggtga ttttgagaac ttgtccggcc tggcccaatc
    15601 cctgatggaa acgtataagg aaaaacctgc cacgcgaatg acccgccggg ttcgcagttt
    15661 gttcggcgga accgaacttc ctcgactcga gggtttggat gacgatgcga cggacgagga
    15721 agaataaccc cggaaactga cagattcctg aatgcactgc cccgattggc cctgatggtt
    15781 tgtcgtcagg gagtcccccc attacaattc ttaatgactg ggatgtggca ataagcccgc
    15841 ctgatagctt atgaataata gtaagaaaca tgagcacggg ccgatcgagc ggccgcgttt
    15901 ttattcattg ctgataaccg tggccgcgtt ggcgtttagc ctgttgtctg ccggctgtgg
    15961 gatcgacttc aacgatccgc ctcgtctggc aacagcgacg gcgcgatcgg ccgcgccgcc
    16021 tgccggccaa gcagaacctt tgatattgcc tgcgccgacc gggacgcccg gatcagcgca
    16081 ggcggaaact gattccagtg caactgaagc gattccggct atcactccta tcagcgaagg
    16141 ggatggtttg gttctttggg tcgacgaaac atcggctgaa cataagcgcg tgcttgacga
    16201 gatagcggcc gaattttctg atcggtatgg cacgaatatg gctgtccagt tggtctctcc
    16261 ggcgattctg cccgatttgg tcagcaccgg ggtcctttcc ggcaccttgc ctgacttgat
    16321 ccttcatccc atcgaatata caaccggctg ggccgagagc ggtattctgg atgcggccgc
    16381 gaccgacgag gttgctgatc ggctcggacg tgagactttt gatagtgccg ctcttgagtt
    16441 ggtgacggta aatggccaga ttgcggcgct gcccagtgat ggctttcatc agcttctcct
    16501 ctatcgtgcc gactggtttg aggaattgaa tctggctgtg cctgacacct ataatgcgat
    16561 cgccgcggcc gccgagcgaa tcaacaaccc cgaagccatc atctccggcc tcgtgatccc
    16621 aaccgaatcc aatctgatca ccactcatca ggcattcgag caaatcgctc ttgccaatgg
    16681 ctgccggtta attgatgaaa atggcgaggt cttgcttctg gagccggaat gcgccgcggc
    16741 gctggagcag tattacacga caattcacca gttcagccca tccggggtgc agacagacac
    16801 cagcgtggct attgcttacc tgaccgggcg aaccggcatc atcatgacgt cccccgctat
    16861 tttgcccgac cttgccggtc tgaatgagcc ggcgatgcct acatgccctg aatgtgttac
    16921 cggagaaggg ggtgttaact atctggccca aaacaccggt attgtgactg aaattcgcgg
    16981 accgaacggc gaaccaacgt catttggcaa catccgcaat ttgggaatta cttccacggc
    17041 cgatcgtgct gcggcccagg ccttcgctga attctggttc aatgagggtt acgagcggtg
    17101 gctggcagtt gattcggagc gcaaggtgcc gatgcgatta gggacgcaga gcgaaccgcg
    17161 tcgatttatc gatgcctggg gaatactgcc tctgggagaa agcagcctga gcttgacgga
    17221 tatatatggt gcggaagttg tggagcgttt gcgaaccggc atcgccgccg cgccgcgctg
    17281 gggaattcgt gaagggtatg gcggtctgat gactcggctc tatgatgatt acgtcatctc
    17341 tattgtcctg caggaaatgt tgagcggcta ttttgatacc acaacgacat tgcgggaagg
    17401 ctatcggcgt actattgaac tgatcccgaa ctatgttttc ccggataggt tggccgaaga
    17461 aaccggagaa aattagtgca tatcgctgct tgacgcattg ccggcagcgt gatatgatgc
    17521 tctcgatatg acaggtcaag aaagccatct ggcaagcttc ttctttttct tttattt
//
LOCUS       DAOMQG010000030        17575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1423_length_17575_cov_10.722603, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000030 DAOMQG010000000
VERSION     DAOMQG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 17575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17575
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_17575_cov_10.722603"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="hrcA"
                     /locus_tag="PLC06_03325"
     CDS             <1..1013
                     /gene="hrcA"
                     /locus_tag="PLC06_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRO89506.1"
                     /translation="VGSKALVEEYGLDFSSATVRNDFAALGELGYVAQLHTSAGRIPT
                     ERGYRYFVQKLLGEFHLPLHERQMIRHQFHQARLELSQWMRLSAAILARTSGSASFVT
                     APQSRANRFKHLQLIATQGRLVLMVLVLFGGEVKQQMLTLAEPLSQHRLSTVADRLNA
                     IFHEKDSDEIAARLSAGQFDTLESEVINLVLDSLRRADHRSISDIYRDGLVNILEDEG
                     TRHAIRLLEERTLLASVLTEQEAQGGEGVQVVIGGDGRWEELKDCTMILSRYGVTESF
                     SGTLAVVGPTRMPYDRNVAAVRYVADLMSSFVYDYFLQETADLPENTLYQIEVDNSDS
                     IE"
     gene            997..1539
                     /locus_tag="PLC06_03330"
     CDS             997..1539
                     /locus_tag="PLC06_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRO89507.1"
                     /translation="MTVSNNEQEWEEVAGGNGVDTATMAGETQSLEEQLAVAQAEAAR
                     NLDGWQRSQAEFANARKRFDKQRAETFVTATIDLVGKLLPLIDDFERALDSVPAIEKN
                     DPWVTGVALVYRKMLSVLDEMNVQVIPSVGEPFDPNQHEALSQEPSDEHQSGVVVREM
                     RRGYRLGDRVIRPALVSVAP"
     gene            1570..3477
                     /gene="dnaK"
                     /locus_tag="PLC06_03335"
     CDS             1570..3477
                     /gene="dnaK"
                     /locus_tag="PLC06_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRO89508.1"
                     /translation="MATIIGIDLGTTNSVVAVMEGGEPVVIPSSEGSRLFPSVVAVNP
                     KTGERLVGQVAKRQGVINSQNTIFSVKRFMGRKFSDPEVQRALKYVPYEVKEASNGDV
                     RVVMNGREYSPPEVSAMILQKIKTDAEAYLGQPVTQAVITVPAYFNDSQRQATKDAGK
                     IAGLEVLRIVNEPTASSLAYGLGSKKDEMIAVYDLGGGTFDISILEVGDGVFEVKSTN
                     GDTFLGGDDFDQKIIDWAADQFKMEEGIDLRNDKQALQRLREAAEKAKVELSTTLQSE
                     LNLPFITADATGPKHMNLTLTRAKLEQLTESLVQRSIEPVRKALKDAGLTVNDITEVI
                     LVGGMTRMPAIQEAVRKFFGKDPHKGVNPDEVVAMGAAIQAGVLGGDVKEVVLLDVTP
                     LTLGIETLGGVATPLIERNTTIPTKKSQVFSTAADSQTQVEIHVVQGERPMAGDNKSL
                     GRFILDGLPPAPRGVPQIEVSFDIDANGILNVSARDKATNRQQAMQIIPSSGLAKDEI
                     DRMMRDAESHAAEDSRRKEEVEARNIADSAVYGAEKFLHDNGDKIPEPQRAAVQTEID
                     AVKQAMSSGDSSGMQKGAERLQQALQQAGAAMYQAGTAEPTADGTADGGPSSNGQSDE
                     DVVEGEFSDAN"
     gene            3554..4714
                     /gene="dnaJ"
                     /locus_tag="PLC06_03340"
     CDS             3554..4714
                     /gene="dnaJ"
                     /locus_tag="PLC06_03340"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRO89509.1"
                     /translation="MASKRDYYEVLGVTRTATKEELKKQYRSLARQYHPDVNGEADAG
                     ERFKEISEAYEVLSDDDKRAAYDRFGHAGVGNGGFGGFDQQGFGGFADIFEEFFGSFG
                     GATGGRRRRHGPRRGPDLRYDLTITFEEAIFGAEREIEYRRAETCPVCSGSGAEPGSR
                     PITCTTCNGSGEVRRVQQSILGQFVNVTTCHTCNGTGELIPDLCKECNGRKQVERTVT
                     KKVKVPAGVDSDTQIRLTGEGGAGLDGGPAGNLFVVLNVAPHEYFQRRGDDIVLDLQI
                     NIAQAALGDEITVPTVDGDMALHIPGGTQSGRVFRMRGLGVPKLDRSGRGQNVGRGDQ
                     LVIINVAIPKNLTTEQQDLFKQLSRTLGKEVVPQKDKGILGQLKDALGDLFG"
     gene            4741..5664
                     /gene="prmA"
                     /locus_tag="PLC06_03345"
     CDS             4741..5664
                     /gene="prmA"
                     /locus_tag="PLC06_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRO89510.1"
                     /translation="MYWLEISVRTDGEGAEAVAEVLRPFAYQESVVLEQLGDEATVDP
                     DALESVVTVKIYVPEEEDTPALRRRVEEILYHMSRLYPFPEPVFRQLADEDWANAWKD
                     HYRPFRLGRRLKIWPTWLPRESDEDVNPDDIVLILDPGMAFGTGLHPTTQGCLRALED
                     GVEPGMSILDAGTGSGILAIAAAKLGATEVAAFDTDTLAVRATVDNAAQNDVADAIHV
                     WRGELDSVRDEVTRASWDIVVANILAPIIIQLLSEKGLLDYVAPKGRLILSGIILEQG
                     PAVEEAIAEAGGQVRDTIVAGDWITYIAGRR"
     gene            complement(5729..5977)
                     /gene="infA"
                     /locus_tag="PLC06_03350"
     CDS             complement(5729..5977)
                     /gene="infA"
                     /locus_tag="PLC06_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRO89511.1"
                     /translation="MANKEDKLEVEGTVIELLPNTQFMVELDNGHRVLSYLSGRMRKH
                     YIRILLGDRVRVEMTPYDLERGRITYRYRRNVQTAAGA"
     gene            complement(6015..7562)
                     /locus_tag="PLC06_03355"
     CDS             complement(6015..7562)
                     /locus_tag="PLC06_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRO89512.1"
                     /translation="MNSLEDLLDQHDYELPQPGDIRTGVIVANTPQGLIIDLGLKRDG
                     IVPQSDLAKLEPEERDNLKVNDEVPVYVVQTEDPEALVVSIHLARLNQDWIEVEALLA
                     SGEIFEGEVIGYNKGGVIVPYGRIRGFVPISHLSDVTPGMGERRRQQRLARIRGEKIG
                     VKIIEVDRHRHRLVMSQREAQKEWEEKKRGELMETLQPGEIRTGHISGMRDFGAFVDL
                     GGADGLVHISELSWHRIDHPREVVKLGDEVEVYVLGVDQESGRISLSRKKLLPNPWDT
                     VAQRYAQNQLVEGKITRILDYGAFAEIEPGVEGLLHVSQLSRNPVEDPRAVVKEGEVH
                     LLRVVSIDQKRQRIGLSLKAVTPTEQIEWMAQKELAEALRIEEEEATNAARDSVRQAE
                     AETDEVVVEEVEETTADVAEDAAEVVDESVDETIDVMESAGDEIVADVTEASEAADDA
                     VEAVAERTEEITIDTAEATEEAKGSVVDKIQDAVSDAVDAAKDAVESIVDKIEDAVED
                     VTTTKAD"
     gene            complement(7912..8778)
                     /locus_tag="PLC06_03360"
     CDS             complement(7912..8778)
                     /locus_tag="PLC06_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRO89513.1"
                     /translation="MKLVTYEHNGNVAIGLLRDETIIDLSAVAPDMIALIESGEQGLA
                     RARAAAETATSALPLSEVRLLPPIHTPRRNVMCVGKNYVEHTRESFEARGETVETIEY
                     PVIFTKTTTCVNGPYDDIPYDAAVSENIDYEAELAVIIGRQVKNTNREEAEAAVFGYT
                     VLNDVTARDLQSLHKQFFKGKSLDGSCPIGPWIVTADELPDPYSLRITCHVNGELRQD
                     SANETMTFDIPTIINHLSRGMTLLPGDIIATGTPSGVGFAMKPPVFLRPGDVVECAIE
                     GIGVIRNRIAAV"
     gene            8927..11926
                     /gene="priA"
                     /locus_tag="PLC06_03365"
     CDS             8927..11926
                     /gene="priA"
                     /locus_tag="PLC06_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRO89514.1"
                     /translation="METAQYAEVVVNVEAALSDAYHYHVPSDMRATLRVGHLVEVEFG
                     RQLAQGIVVAFAGSAPVEETKPIISLIDPEPVLWPWQIDLARWLSHRYMAPLNACFRL
                     LLPPGLTRWADSVYDINPYWDGEGRLTDVQRQLVDLLRERGDLRGRQISRVIRKKKWQ
                     AAAALLVNRSILRRASVLDPPRARPKHIRTVELIAGPNRIWAAAAELGRASKSADILH
                     YLAESKDPLPVEADVLAATGATRKHMTALVDEGLVARFAPAEVVIPVPGSDVPAAEAD
                     LVAQLPLSRADLPRPDLLPRWLDVGLVRLETTPPSAGLSIPPRQVMGQVLRMRKTEVY
                     GRVLDFLADEARAVPVGEVYAATGATLTQLRRLAELDLVRLGDAHVWRNSLADRDFAP
                     ADPPELTLDQARVWGRIKVAMIEADDGALDDSWDEEDGASEIDMARDGDALDPMDAAT
                     PFLLHGVTGSGKTEIYMRAIDLALSRGQQAIVLVPEIALTPQTVRRFAARFPGRVAVL
                     HSALSDGERYDTWRRARAGLFDIVIGPRSALFAPLPNVGVIVVDEEHDGSYKQTPPIP
                     PPYYHAREAAIALAGFTGAVVILGSATPDVVTYHRAQSGRYQLLELPKRIMGHRRRIE
                     SQAERLHIESRYRPLTDGGEGHDILTMDEALTIPLPPIQVVDMRQELRAGNRSVFSRA
                     LDAAVSETLARGEQTILFLNRRGSATFVNCRDCGHVMNCPKCGIPLTYHQPNMTLVCH
                     QCGRREPQPHACPNCGSERIRYFGLGTERLEEMVRARWPEARLVRWDRDTTSEEGSHE
                     ALLAGFINRESDILVGTQMIAKGLDLPFVTLVGVVSADIALGLPDYNTGERVFQVLAQ
                     VAGRAGRGLLGGRVVVQTYTPEHYAIQAAAEHDYAGFYLEEIRFRTQRALPPFRRIVR
                     LLISDPISDRAERMAKEMAQVLNLTVRERALAATEILGPTPAFFTRLDGRYRWHIVVH
                     TPDPHRLLEDVPIPRPWVVDIDPESTL"
     gene            complement(12040..12111)
                     /locus_tag="PLC06_03370"
     tRNA            complement(12040..12111)
                     /locus_tag="PLC06_03370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12076..12078),aa:Arg,seq:cct)
     gene            12151..12585
                     /locus_tag="PLC06_03375"
     CDS             12151..12585
                     /locus_tag="PLC06_03375"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRO89515.1"
                     /translation="MPRSTPYLPFFVYGTLLPGEANFRLWRDAIQDIKPAGLNGARLY
                     DLGYFPMLIEGGGGEVRGMVGWVRPSSYGAALDLLDTLEGVRFPYPIGVGYYRAARVV
                     RLAGGSTVAAWVYLGNPVAVIGRRPIGTDWKTYRRKSFKFRY"
     gene            12798..15044
                     /locus_tag="PLC06_03380"
     CDS             12798..15044
                     /locus_tag="PLC06_03380"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRO89516.1"
                     /translation="MKRIGVLTSGGDAPGMNAALRAVVRAGLNRGMEVFAIYEGYSGM
                     VEGGERIRLMSWTDVGGILHLGGTVIGSARCKDFRERSGRLRAVRNLLLNEIEGLIVI
                     GGDGSLTGAALLHDEWSGLIEELVESGQVTRKLAARNKSLPVVGIIGSIDNDMFGSDI
                     TTGADTALHRIIDATDAISSTAASHQRSFVVEVMGRNCGYLALMAAMASGADWVLIPE
                     SPPDVDDWEARMCEKLRMGRQIGRRDSMVIVAEGARDRNGQPITSRHVKEVLEERLGE
                     DVRITVLGHVQRGGSPSAFDRNLSTMLGVEAIETIATMGPDVAPLIVGMQGNKLTRTP
                     LQVALEKTKAVADAVAAKRFDEALALRGPTFGKSFEVVRTLVRASPRPPDPDQRRLRI
                     AVMHGGGPAPGMNTAARAAVRIGIDRGHIMLGVRNAFRGLVNNEIIDLDWMSVNGWAT
                     RAGSELGTNRTIPKGRDLYAIARNLEEHRIDGILMIGGWSGYQSILELHNQRKNYPAF
                     DIPMLCLPATINNNLPGTELSVGADTALNSIIMAVDKIKQSAVASQRAFIVEVMGYYC
                     GYLSLMGALATGAERVYLHEEGVTMRDLVTDVEEFREAFERGKRLGLVIRNEMANEVY
                     TTNFMRMLFEEEGGDLFDVRQAILGHMQQGGTPSPFDRIFATRLGVTCIDSLVERITN
                     QEKAGACVGQVGGEIKFTDLDDIPRLMDYVHSRPIKQWWMNLIPIARLLARRNGVAPD
                     DIGAGQPG"
     gene            15093..16259
                     /locus_tag="PLC06_03385"
     CDS             15093..16259
                     /locus_tag="PLC06_03385"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRO89517.1"
                     /translation="MRIGILTGGGDVPGLNPCIKAVVNRAISAGHEVIGIRRGWGGLL
                     LVDRDDPATIEKYILPLDNSVVRTIDRTGGTFLHTSRTNPGKVKPDSVPEFLKDPNHP
                     EAEAADNSPRDFTPHVLKNLEYLKIDRLIPIGGDDTLSYGERLHREGFPVVAIPKTMD
                     NDVYGTDYCIGFSTAVTRSVQFINQLRTSAGSHERIAVIELFGRNSGETSLISSYLAG
                     VDRALISEVPFDPEHLAELVAKDKKANPSNYAMVTVSEGAHMVGGKVVEYGETDAYGH
                     RKLGGIGEITGEALKKLTGENIITQRLAYLMRSGAPDALDLMVGTNYAQLAVDLIVGG
                     SSGRMVALRDGAYTHVAMSVVTSGVKRVDVDELYDAQNYRPKVRHVLGKPMFLY"
     gene            16495..>17575
                     /locus_tag="PLC06_03390"
     CDS             16495..>17575
                     /locus_tag="PLC06_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRO89518.1"
                     /translation="MYRFHRSPLFLFIVILALSALACQALSIDPSATTPPAPSQQELV
                     DQAVATVMAQIPQASALPPVTTSQSAPISTDIESSLIELYKRVNPAVVHIYVIDEQSF
                     VLGTGSGFVYDELGHIVTNNHVVTGASGLEVVFPDGTRRSAEVVGTDVDSDLAVIRVD
                     DLPAGVLPIPLGDSDNAQVGQFVVAIGNPFDEAGSLSLGIISGLGRSLSSDRIAEGGG
                     RYSLPQVIQTDAAINPGNSGGPLLNLAGEVVGVNSAIRTSTGTNSGVGFSIPVNAVRR
                     VAPALIAEGVYHYPYIGIRMTSLDLASQQAMGLPQAAGAYVIDMTPNSPAQRAGLIAS
                     GFNDTVGTLPGGDLIIAVDGRPISSP"
BASE COUNT         3412 a         5158 c         5385 g         3620 t
ORIGIN      
        1 cggtcggctc gaaggcgctt gtcgaggaat atgggctgga ttttagttcc gccaccgtgc
       61 gcaacgactt tgccgcgctg ggcgagttgg gctacgtggc ccagttgcac acgtcggcgg
      121 gacgaatccc gactgaacgc ggctatcgct acttcgtgca gaagctgttg ggtgaattcc
      181 acctgccgct gcacgaacga cagatgatcc gccaccagtt tcatcaggcg cgcctggagt
      241 tgagccagtg gatgcgactg tcggccgcta ttctggcccg cacatccggc agcgccagct
      301 tcgtcaccgc cccccaatcc cgcgccaatc gcttcaagca tctccagctc atcgccaccc
      361 agggacgatt ggtcctcatg gtgctggtgc tgtttggcgg cgaagtgaaa caacagatgc
      421 tcacgctggc cgaaccatta tcgcaacatc gcttaagcac ggttgccgat cggctcaacg
      481 cgatttttca cgagaaagac tccgatgaga tagccgcgcg cctaagcgcc ggtcagttcg
      541 acacgctgga aagcgaagtg attaacctgg tgctcgattc cctgcgccgt gccgaccacc
      601 gttccatcag cgatatctat cgcgacgggc tggtcaatat cctcgaggac gaaggcactc
      661 gtcatgcgat tcgcttgctg gaggaacgga cgctgttggc gtcggtcctc accgagcagg
      721 aggcgcaggg cggcgaaggc gtccaggtgg tcatcggtgg tgacggtcgg tgggaggagc
      781 tgaaggattg caccatgatc ctctcccgct acggcgtgac cgagagcttc tccggcacgt
      841 tggccgtcgt cggtcccacg cgcatgccct acgaccgcaa tgtggccgcc gtgcgctatg
      901 tggccgatct gatgagcagt tttgtatacg attatttcct acaggaaacg gctgatttgc
      961 ccgaaaacac cctgtatcaa attgaggttg ataacagtga cagtatcgaa taacgaacag
     1021 gaatgggaag aagtagccgg cggcaatggc gtcgacactg ctaccatggc tggtgaaacc
     1081 caatctttgg aagaacaatt ggcagtggcc caggccgaag ccgcgcgcaa tctggacgga
     1141 tggcagcgat cccaggcgga atttgccaat gcccgcaagc gttttgacaa gcaacgcgcg
     1201 gaaacgtttg tgaccgcgac aatcgacctg gtcggcaagc tgctgccgct catcgatgat
     1261 tttgagcgcg cgcttgattc ggtccccgca atcgaaaaaa acgatccatg ggtcaccggc
     1321 gtcgccctgg tctatcgcaa gatgctgtcg gtgctggacg agatgaacgt gcaggtcatc
     1381 cccagcgttg gcgagccttt cgatccgaac cagcacgagg cattgtctca ggagccttca
     1441 gacgaacacc agagtggcgt cgtcgtgcgt gagatgcggc gcggttaccg gttgggcgac
     1501 cgggtgatcc gcccggcgct tgtttccgtc gccccctaac ccgcaattat taaatagagg
     1561 atatcttcaa tggcaacgat aattggtatc gatttgggca caacgaactc cgttgtcgcc
     1621 gtgatggaag gtggggagcc tgttgtcatc cccagctcgg aagggagtcg cttgttcccc
     1681 tccgtggtcg ccgtgaaccc caagacgggc gagcgcctgg tgggccaggt cgccaagcgg
     1741 caaggcgtca tcaattccca gaacaccatc ttctcggtca agcgcttcat gggtcgcaag
     1801 tttagcgacc ccgaagtgca gagggcgctc aagtatgtgc cttacgaggt gaaggaagcg
     1861 tccaacggcg acgtgcgcgt cgttatgaac ggccgcgagt attcgccgcc ggaagtctcg
     1921 gccatgatcc tgcaaaagat caagaccgac gccgaggcct atctgggcca gcctgtgact
     1981 caggccgtca tcaccgtgcc ggcctatttc aacgacagtc agcgccaggc caccaaagac
     2041 gcgggcaaga tcgccggtct ggaagtgctg cgcatcgtca atgagccgac cgcctcctct
     2101 ctggcctatg gcttggggag caagaaagac gagatgatcg ccgtctatga cctgggcggc
     2161 ggcacgttcg acatctcgat tcttgaggtg ggcgacggcg tcttcgaggt gaaatcgacc
     2221 aacggcgata ccttcctggg cggcgacgac ttcgaccaaa aaataatcga ttgggcggcc
     2281 gaccagttca agatggaaga gggcatcgac ctgcgcaacg acaagcaggc gctgcagcgc
     2341 ctgcgcgagg cggccgagaa ggccaaggta gagctgtcga cgacgttgca gagcgagctg
     2401 aacctgccgt tcatcaccgc cgacgcaacc ggcccgaagc acatgaacct gacgctgacc
     2461 cgcgccaagc tggagcagct gaccgagtcg cttgtgcagc gctccatcga gccggtgcgc
     2521 aaggcgctca aggatgccgg tctgaccgtc aacgacatca ccgaggtcat cctcgtcggc
     2581 ggcatgaccc gcatgcccgc cattcaggaa gccgtccgta aattcttcgg caaggatccc
     2641 cacaagggcg tcaaccccga tgaagtcgtc gccatgggcg cggccattca ggccggtgtg
     2701 ctgggcggag atgtcaagga agtcgtcctg ctcgatgtga ccccactgac gctgggtatc
     2761 gagacgttgg gcggggtggc tacgccgctc atcgagcgca acacgaccat ccctaccaag
     2821 aagagccagg tgttctccac ggcggccgac agccagaccc aggtggaaat ccacgtcgtg
     2881 cagggcgagc gcccgatggc cggcgataac aagagtctgg ggcgcttcat cctcgatggg
     2941 ttgccgcccg cgccgcgtgg cgtgccgcag atcgaagtgt cgttcgacat cgacgccaac
     3001 ggtatcctca acgtcagcgc gcgcgacaag gccacgaacc gccagcaggc gatgcagatc
     3061 atcccctcca gcggtttggc caaggatgag atcgaccgca tgatgcgcga cgccgagagc
     3121 cacgccgcgg aagacagccg ccgcaaggaa gaagtcgaag cgcgcaatat cgccgactcg
     3181 gccgtctatg gcgccgagaa gttcctccac gacaacgggg acaagattcc tgagccgcag
     3241 cgcgcggccg tgcagaccga gatcgacgcc gtcaagcagg cgatgtccag cggcgactcg
     3301 agcgggatgc agaagggggc cgagcgcttg cagcaggcat tgcaacaggc cggcgcagcc
     3361 atgtatcagg ccggcacggc cgaaccgacg gccgatggga cggccgatgg tggcccttcg
     3421 agcaatggcc aatccgatga agacgtcgtc gaaggcgaat tcagcgacgc taactaaaaa
     3481 tgagtatgga ttaggaatta cgacgtctca aactcgtaat tcctgattcc taatccgtaa
     3541 ttcctaattc tctatggctt ccaagcgcga ttactacgaa gtgctgggcg tgacccggac
     3601 ggcgaccaaa gaggaactga agaaacagta ccgtagtttg gcgcgccagt atcatcctga
     3661 tgtgaatggc gaagccgacg caggcgagcg cttcaaggag atcagcgagg cctatgaggt
     3721 tttgtctgat gacgacaagc gcgccgccta tgatcgcttc ggtcatgccg gtgtcggcaa
     3781 cggcggattt ggcgggttcg accagcaagg gtttggcgga ttcgctgata tcttcgagga
     3841 gttcttcggc tcatttggcg gggccaccgg cggccgtcgc cggcggcatg gtcctcgtcg
     3901 cggccccgat cttcgctatg acctgacgat cacttttgag gaagcgatct tcggcgcgga
     3961 gcgggaaatt gaataccgtc gcgcagagac atgccccgtt tgcagcggca gcggcgccga
     4021 gcctggctca cggccgatca cctgcacgac atgcaatggt tccggggaag tccggcgcgt
     4081 gcagcagtcg atcctgggcc agtttgtcaa tgtgacgacc tgccacacct gcaacggcac
     4141 gggcgagctg attcccgacc tttgcaagga atgcaacggc cgcaagcagg tggagcgcac
     4201 cgtcaccaag aaggtcaagg tccccgccgg cgtggatagc gacactcaga ttcgcctgac
     4261 gggcgagggt ggtgcaggtc tggatggcgg cccggccggc aacctgtttg tcgtgctcaa
     4321 cgtggctccc cacgaatact tccagcgccg gggcgatgat atcgtcctcg acctgcagat
     4381 caacattgct caggcggcat tgggcgatga gatcaccgtt ccgacggtag atggggatat
     4441 ggccttgcac atccctggcg gtacacaatc cggccgcgtt tttcgcatgc gcgggctggg
     4501 cgtccccaag ctggatcgca gcggccgtgg ccaaaatgtg ggccggggtg accagttggt
     4561 gatcatcaac gtggccattc ccaaaaacct caccactgag cagcaggact tgttcaagca
     4621 actctcgcgc acgttaggca aggaagttgt gccgcagaaa gacaagggca tcctgggaca
     4681 attaaaagac gcgctgggcg acctgttcgg ctaatttata gccgcaggag cgagagaatt
     4741 atgtactggc tggaaataag cgtgagaacc gacggcgaag gcgccgaggc cgtggcggaa
     4801 gtgctgcggc cttttgctta tcaggagagc gtcgtcctgg aacaactggg cgatgaagcc
     4861 acggtcgatc ccgacgcgct ggagtccgtg gtcaccgtca aaatctacgt ccccgaggaa
     4921 gaggatacgc ctgctttgcg ccggcgggtt gaagagattc tgtatcatat gtctcgcctc
     4981 taccccttcc cggagccggt cttccgccaa ttggccgatg aggattgggc caatgcctgg
     5041 aaggatcact accgcccgtt tcgcctcggt cgccgtctga agatttggcc cacgtggctg
     5101 ccgcgtgaat cggatgagga tgtcaatccc gatgacatcg tgctgatcct cgatccgggt
     5161 atggcgttcg gcacggggct gcatccgacg acgcagggtt gcctgcgcgc gctggaagac
     5221 ggagtcgagc cgggaatgag tatcctggac gcgggcacag ggtcgggcat tctggccatc
     5281 gccgcggcca agctcggggc gactgaggtg gccgcctttg acaccgacac tctggccgtt
     5341 cgggcgacgg tcgataacgc cgctcaaaac gacgtcgccg acgccattca tgtctggcgc
     5401 ggcgaactgg acagcgtgcg cgatgaggtt acgcgcgcgt catgggatat tgtcgtggcc
     5461 aatatcctcg cgccgatcat cattcagttg ttgtcggaaa aagggctact tgactacgtg
     5521 gctcccaagg gccggctcat tctgagcggc atcattctgg agcaggggcc ggcggtggaa
     5581 gaggcgattg ccgaggcagg cggacaggtg agagacacca ttgtcgccgg ggactggata
     5641 acctacatag ccggccggcg ctgaggcgcg cggccggctg taagatttca gatcatgttg
     5701 acggggactg cccccgcatc gtaaagggtt aggctccggc ggcggtctga acgttacggc
     5761 gatatcggta ggtgatccgg cctcgctcca ggtcataggg tgtcatctcg acccgcacac
     5821 ggtcgcccag caagatacga atgtaatgct tgcgcatgcg accggacagg tacgacaaga
     5881 cccgatgacc gttgtccaac tcgaccatga actgcgtgtt gggcagcagc tcaatgacgg
     5941 tgccctcaac ctctaacttg tcttctttat tggccataaa ctcctgactt tcttttctgc
     6001 aagaccgttt ttatctagtc tgccttggtg gtcgtgacgt cctcgaccgc gtcttcgatc
     6061 ttgtcgacga tgctttcaac ggcatccttg gcggcatcca ctgcatcgga cacggcatcc
     6121 tgaatcttgt ccactacgga ccctttcgct tcttcggtgg cctcagccgt gtctatggtg
     6181 atttcttcgg ttctctcggc tacggcctca accgcgtcgt cggccgcctc ggacgcttcg
     6241 gtaacgtcgg caacgatctc atcaccggcc gattccatca catcaattgt ttcatcgaca
     6301 gactcgtcaa cgacttcggc cgcatcctcg gcgacatcgg cggtcgtttc ttccacttct
     6361 tcaaccacga cctcgtcggt ctcggcctcg gcctggcgaa cggagtcgcg ggcggcattg
     6421 gtcgcctctt cttcctcgat tcgtaacgct tcagccagct ctttctgggc catccattcg
     6481 atctgctccg tcggcgtcac ggctttcagg ctcaggccaa tgcgttggcg cttctgatcg
     6541 atgctgacca cgcgcagcag gtgaacttcg ccttccttaa cgacggcccg tgggtcttcg
     6601 accggattgc gggagagctg tgaaacgtgc agcaggcctt cgacgcccgg ctcaatctcg
     6661 gcgaacgcgc cgtagtcgag tatgcgcgtg atcttgccct cgaccagttg gttctgggca
     6721 tagcgttgcg ctaccgtgtc ccatggattg ggcagcagtt tcttccggct gaggctgatc
     6781 cggccggatt cctggtcgac gccgaggacg tagacttcaa cctcgtcgcc cagtttgact
     6841 acctcgcgcg ggtggtcgat gcgatgccac gacagctcgg agatgtggac gaggccgtcg
     6901 gctccaccca aatccacgaa cgcgccgaaa tcgcgcatgc cgctgatgtg gccggtgcgg
     6961 atttcgccgg gctgcaacgt ctccatcagt tcaccgcgct tcttctcttc ccattctttc
     7021 tgcgcttcac gctgggacat gaccaggcga tggcgatggc gatccacttc gatgatcttg
     7081 acgccgatct tctcgccacg gatgcgggcc agtcgctgct ggcggcgccg ctcgcccatg
     7141 ccgggcgtga cgtcgctcag gtgggagatg ggcacgaaac cgcgaatgcg gccgtagggc
     7201 acgatgacgc cgcccttgtt gtagccaatc acttcgcctt cgaagatttc tccgctggcg
     7261 agcagggctt cgacctcgat ccagtcctga ttaaggcggg ccagatggat ggataccacc
     7321 agagcttccg ggtcttccgt ttgtacaacg taaaccggaa cctcgtcatt cactttaagg
     7381 ttgtcgcgct cttccggctc cagcttggca agatcagact ggggcacaat cccatcgcgc
     7441 ttcaaaccca gatcgatgat taggccttgg ggtgtgttgg cgacaatcac gcctgtgcgt
     7501 atatccccgg gttgaggtaa ctcatagtcg tgttggtcca gcagatcttc tagagagttc
     7561 atcgattcgt cagacatacc ttcgtcctaa aaggcttacg ttttgggtct catccaaata
     7621 taacaataaa aaactcggcc catccgttgc gatatatggc aaccaatggc cgagttcgct
     7681 gcgcctgatc cacactgacc gacgccattg cagacaatga agggcggcta gctatccctt
     7741 aagcacaaag cttgtttgat ttttcatcga gaaaccccca gaataacgta cgagtcgcct
     7801 cttgtggttt cgacaatcgg tcttctaagc cgtcctaccc tgacccactc agccaactcc
     7861 cgattatatc atcaagggcc ggatagtcaa tccgccggga acggccgcgc tctaaacggc
     7921 ggcaattcga ttacgaatca cgccgatacc ttcgatcgcg cactcaacca cgtcgcccgg
     7981 tcgcagaaat accggcggct tcatggcgaa gcccacgccg ctgggggtgc cggtggcgat
     8041 gatgtctccc ggcaagagcg tcatgccgcg cgacagatga ttgataatgg ttgggatgtc
     8101 gaaggtcatc gtctcgttgg cgctgtcctg gcgcagctcg ccgttgacgt ggcaggtgat
     8161 gcgcaggctg taggggtcgg gcagctcgtc ggccgtcacg atccatggcc cgatggggca
     8221 actgccgtcg aggcttttcc ccttgaagaa ctgtttatgg agcgattgca gatcgcgggc
     8281 ggtcacatcg ttgaggacgg tgtagccaaa gacggccgcc tctgcctctt cgcgattggt
     8341 gtttttcact tgccggccaa tgatcacggc cagctcggcc tcgtaatcga tgttctccga
     8401 tacggccgcg tcgtagggga tatcatcata ggggccgttg acgcaggtgg tcgtcttggt
     8461 gaagatgacc ggatattcga tcgtttccac tgtctcgcca cgggcctcaa acgattcgcg
     8521 ggtgtgttcg acatagttct tgcccacgca catgacgttt cggcgggggg tgtgtatggg
     8581 gggcagcagc cggacttcgc tcagggggag ggcactggtg gcggtttcgg ccgctgcccg
     8641 cgcccgggca agtccttgtt ctcctgattc gattaacgcg atcatgtcgg gagctactgc
     8701 cgacaggtcg atgatagttt catcgcgcag caagccaatc gctacgttgc cattgtgttc
     8761 ataggtgact agtttcacgt gattcctgtt tcttttactc tctgagggat tactctggca
     8821 aattgtacac cggcgggcca attcggagaa atagcggata tcaaaacgat tgtaacctga
     8881 tatgaaacat atgttcttgt ttgaccgttg aggtacaatc aagagtatgg aaacggccca
     8941 atatgcggaa gtcgtcgtca atgttgaggc ggcgctatcc gacgcctatc attaccacgt
     9001 accttccgat atgcgggcga cattgcgtgt cgggcacttg gttgaggtgg aattcggccg
     9061 ccaattggcg caggggatcg tcgtcgcctt tgccgggagc gccccggtgg aagagaccaa
     9121 gcccatcatc agcctgatcg atcctgagcc ggtgttgtgg ccatggcaaa tcgatctggc
     9181 ccgctggctc agccatcgct acatggctcc gctcaacgcc tgcttccggc tgctgctgcc
     9241 gccgggcctg acgcgctggg ccgatagcgt gtacgacatc aatccctatt gggacggcga
     9301 gggacgtctg accgacgttc agcgacagct ggtcgacctg ctccgcgagc ggggcgatct
     9361 gcgcggccgg cagattagtc gggtcatccg gaaaaagaag tggcaagcgg ccgccgcgct
     9421 gctggtcaat cgatcgatcc tgcgccgagc ctcggtgttg gacccgccgc gcgcgcggcc
     9481 aaagcacatc cgcactgtcg aactcattgc cgggccaaac cgcatttggg ccgccgctgc
     9541 tgagttggga cgggccagta aatcggccga tatcctgcat tatctggccg aatcgaagga
     9601 cccgctgccg gttgaggctg acgtgttggc cgccactgga gctacgcgca agcatatgac
     9661 cgcgctggtc gacgaagggc ttgtggctcg ttttgctccg gccgaagtcg ttatccctgt
     9721 gccgggaagc gatgtccccg cggccgaagc cgatctggtc gcccaattgc cgctatcacg
     9781 cgccgacttg ccccggcctg acctgctgcc ccgttggctg gatgtgggtc ttgtccgcct
     9841 tgagacgacc cctccctcgg caggcctgtc catccctccg cgacaagtga tggggcaggt
     9901 cttgcgcatg cgcaagactg aagtctatgg ccgcgtcctg gactttctgg ctgatgaggc
     9961 gcgcgcggtg cctgtgggcg aggtgtatgc cgcgaccggg gcaacgttga cccaactgcg
    10021 gcggctggcc gaactcgatc tcgtacggct gggcgacgcg cacgtctggc gcaattcact
    10081 ggccgatcgc gacttcgcgc cggccgaccc gccggagctg acgctggatc aggcgcgggt
    10141 ctggggccgc atcaaggtcg ccatgatcga ggctgatgac ggcgcgcttg atgattcatg
    10201 ggatgaagaa gatggcgcct cggaaatcga catggcgagg gatggcgacg cgctcgaccc
    10261 gatggacgcg gccacgcctt ttctgcttca tggcgtcacg ggcagcggca aaaccgaaat
    10321 ctatatgcgg gcgatcgatc ttgctctctc tcgtggccaa caagccatcg tcctggtgcc
    10381 cgaaattgcc ctcacgcccc agactgtgcg ccgatttgcg gcgcgatttc ccggccgggt
    10441 ggcggtgctc catagcgccc tttccgatgg ggaacgctat gacacctggc ggcgggcgcg
    10501 ggccgggctg tttgatatcg tcatcggccc gcgcagcgcc ctgttcgcgc cgctgccgaa
    10561 cgtgggtgtc atcgtcgtcg atgaagaaca cgacggcagc tataagcaga cgccgcccat
    10621 cccgccaccc tattaccatg cgcgagaagc ggccatcgct ctggccgggt tcaccggcgc
    10681 ggtggtcatt ttgggcagcg ctacgcccga cgtcgttacc tatcatcggg cacaatcagg
    10741 acgctaccaa ttgctggaat tgccgaagcg gatcatgggc catcgtcgcc gcatcgagag
    10801 ccaggcagag cgtttgcaca ttgaaagccg ctatcggccg ctgaccgacg gtggcgaagg
    10861 gcacgatatc ctcaccatgg acgaggccct gaccatccca ttgccgccta tccaggtggt
    10921 ggatatgcgc caggagttgc gggccgggaa ccgctcggta tttagccggg cgctcgacgc
    10981 ggctgttagc gagacgctgg cgcgcggcga gcaaacgatc ctgttcctga accgtcgtgg
    11041 ctcggcgacg ttcgtcaatt gccgtgactg tggtcatgtg atgaactgcc cgaaatgcgg
    11101 catccctctc acctatcatc agcctaacat gacgcttgtc tgccatcaat gcggcaggcg
    11161 tgagccgcaa cctcacgcct gccccaactg cggctccgag cgtattcgct attttgggct
    11221 gggcacggag cggctggagg agatggtgcg ggcgcgctgg cccgaagcgc gccttgtccg
    11281 ctgggaccgc gacacaacga gcgaggaggg atcgcatgag gcgttgctgg ccgggttcat
    11341 caatcgtgaa tccgatattc tggtcggtac gcagatgatc gccaaggggc tggatttgcc
    11401 gttcgtcacg ctggtgggcg tcgtatcggc cgacatcgcc ctggggttgc cggactacaa
    11461 caccggcgag cgcgttttcc aggtgttggc gcaggtagcc gggcgagcgg ggcgtggtct
    11521 gttgggcggg cgggtggtgg tgcaaaccta cacgccggaa cactacgcca ttcaggccgc
    11581 ggccgaacat gattacgccg ggttctacct ggaggagatc cgctttcgca cgcaacgggc
    11641 gctgccgccg tttcgccgaa ttgtgcgctt gctaatcagc gacccgatca gcgatcgcgc
    11701 ggagcggatg gccaaggaaa tggcgcaggt gctgaacctg actgtgcgcg agcgagcgct
    11761 ggcggcaacg gagatattgg ggccgacgcc ggccttcttc acgcgactcg acggccgcta
    11821 tcgctggcac atcgtcgtcc acacccctga tccccatcgg ctgctggaag acgtaccgat
    11881 tccccgaccg tgggtcgtcg acatcgaccc ggagagtacc ttgtagatga aggggaattt
    11941 gaaaaataac ttatgcgagg actgtcgttt aggccaagac ctttttcaac ttcttgactc
    12001 gtaatcccta attcgtaatt cgtaattccc gattattatt gtccccgcac ggactcgaac
    12061 cgtgatctat cgcttaggag gcgattgctc tatcctttga gctacgagga cgcggaaccc
    12121 attttagcca aatcggcgag gagcggctac ttgccccgat cgactccata ccttcccttt
    12181 tttgtctatg gcacattgtt gcccggcgag gccaactttc gactgtggcg agatgccatc
    12241 caggacatca agccggctgg cctgaatggt gcgcgcctct atgatctggg gtattttccc
    12301 atgctgatag aggggggcgg cggagaagtg cggggcatgg tcggttgggt cagaccttct
    12361 tcctatggtg ctgctctgga tctgcttgac acgctggagg gcgttcgctt tccttatccg
    12421 atcggcgtcg gctattatcg tgcggcgcgc gttgtgcgcc tggcgggcgg gtccactgtc
    12481 gcggcatggg tctatctggg caaccctgtg gcggtcatcg gtcggcgacc cattggcacg
    12541 gattggaaaa cctaccggcg aaagagcttc aaattccggt attaatggtt gccgcatggg
    12601 ctattccaag tagtattagg tagttattcc cactccactg tacgctgata gtctataatt
    12661 gtgtgatcac caagcctgcg cgcattgcga ttggcttggg ttgctacttc gcccctcatt
    12721 tggccggaat gtttcgttag taggtcatat tttggaagcg gggtgcaagg ctaaacattg
    12781 ataacgatgg aaaatccatg aagagaatag gtgttttaac gagcggtggg gatgctccgg
    12841 gcatgaatgc cgcccttcgc gctgttgttc gcgcgggtct gaatcgcggg atggaggtat
    12901 ttgccatcta cgaagggtac tcaggtatgg tcgaaggcgg cgaacgtatc cgcctgatgt
    12961 cctggactga cgtcggcggt atcctgcatc tgggtggaac cgtgatcggt tcggcgcggt
    13021 gcaaggattt tcgcgagcgc tccggccgtt tgcgcgccgt gcgcaacctg ttgctcaatg
    13081 aaatagaagg gttgatcgtc attggtggtg acggcagcct aaccggcgcg gcgttgctcc
    13141 acgatgaatg gagcgggctg atcgaagagc tggtagaaag cggtcaggtg acccgaaaac
    13201 tggcggccag gaacaaatcg ttaccggtcg tgggtatcat cggctccatc gacaacgata
    13261 tgttcgggag cgacatcacg acgggagccg ataccgcctt gcatcgaatc atcgacgcca
    13321 cggacgccat ctccagcacg gccgccagcc accagcgcag cttcgtcgtt gaagtgatgg
    13381 gccgcaattg cggctatctg gccttaatgg ccgccatggc ttccggcgcg gactgggtac
    13441 tgattcctga atcgccgcct gacgtggacg attgggaggc caggatgtgt gagaagttgc
    13501 ggatgggtcg ccagataggc cggcgcgact cgatggtcat tgtggctgaa ggcgcgcgcg
    13561 atcggaatgg tcagccgatc accagccgtc acgtgaaaga ggtgttggaa gagcgtctgg
    13621 gcgaggatgt gcgcatcacg gtcctcggcc atgttcagcg cggcggctcc cccagcgcct
    13681 tcgaccggaa tttgagcacc atgctcggcg ttgaagcgat tgagaccatc gccacgatgg
    13741 ggcctgacgt ggcgcctttg atcgtgggca tgcagggcaa caaactgacc cgcacgccgc
    13801 ttcaggttgc tctggagaaa accaaggccg tggctgatgc tgtggccgct aaacgattcg
    13861 atgaagcgct ggcgctgcgc gggccgacgt tcggcaaatc cttcgaggtt gttcgcaccc
    13921 ttgttcgagc ctcaccccgc ccacctgatc ccgaccagcg acgtttacga atcgccgtta
    13981 tgcatggtgg tggccctgca cccggcatga ataccgccgc tcgcgccgcc gtccgtatcg
    14041 gcatcgaccg cggccacatt atgctcggcg tgcgcaacgc gtttcgcggc ctggtcaata
    14101 acgagatcat cgaccttgac tggatgagcg tcaacggttg ggccacgcga gccggttcgg
    14161 aattgggcac gaaccgcacc attcccaagg gacgcgatct gtatgcaatt gcccgaaacc
    14221 ttgaagagca ccggatcgac ggcattctga tgatcggcgg ttggtcgggc tatcagtcca
    14281 tccttgaatt gcacaatcag cggaagaact acccggcatt cgacattccc atgctctgcc
    14341 tgccggcgac gatcaacaat aacctgcccg gcacggagct gagcgtcggc gcggacacgg
    14401 cgctgaacag catcatcatg gcggtggaca aaatcaagca atcggccgtg gcgtcccaga
    14461 gggctttcat cgtagaggtg atgggctact attgtggcta tttatcgctg atgggcgcgc
    14521 tggccacggg tgcggaacgc gtttatctgc acgaagaagg cgtgaccatg cgcgacctcg
    14581 tgacggatgt cgaggagttt cgcgaggcct tcgagcgcgg caagcgactg ggcctcgtca
    14641 ttcgcaatga gatggccaat gaggtctata ccaccaactt catgcgcatg ctcttcgagg
    14701 aggagggtgg cgatctgttc gacgtgcgcc aggcgattct gggccacatg caacagggag
    14761 gcaccccctc gccgtttgac cgtatctttg ccacgcggct gggcgttacc tgtattgatt
    14821 cgcttgtaga gcgcattacc aatcaggaaa aggcgggagc gtgcgttggt caggtgggtg
    14881 gcgaaataaa attcaccgat ctggatgata taccccgtct gatggactat gtccacagcc
    14941 gcccgattaa gcaatggtgg atgaatttga taccgattgc ccgcctgctg gctcgccgga
    15001 acggcgtcgc tccggatgat atcggggccg gacaacccgg ataatttgtt cgtatattat
    15061 cgtatcgatt tcatagaagg agattgatta gcatgagaat tggaattctg acaggcggcg
    15121 gcgatgtccc cggcctgaat ccctgtatta aggcggtcgt gaatcgcgcc attagcgcag
    15181 gccatgaagt gatcggcatt cgtcgtggat ggggtggtct gctcctggtc gatcgggatg
    15241 acccggcgac cattgaaaag tacatcctgc cgctggacaa cagcgtcgtt cgcaccatcg
    15301 accgcaccgg cggcacattc ctgcacacca gccgcacgaa ccccggcaag gtgaagccgg
    15361 acagtgtgcc tgaatttctg aaggacccga atcaccccga agccgaagcg gccgataaca
    15421 gcccacgtga cttcaccccc catgtgctga agaacctgga atatctcaag attgaccggt
    15481 tgatccctat cggcggtgac gatacgctca gctatggcga gcgtctgcac cgtgagggtt
    15541 ttcccgtggt tgccatcccc aagacgatgg acaatgacgt ctatggcacg gactattgca
    15601 tcggtttcag cacggccgtc acccgcagcg tgcaattcat caatcagttg cgcacgagcg
    15661 ccggctcgca cgagcgcatc gccgttatag agctgttcgg gcgtaactcc ggtgagacca
    15721 gcctcatcag ttcctatctg gcgggcgtgg atcgcgcgct catctctgag gtgccgttcg
    15781 acccggagca tctggccgaa ctggtcgcca aagacaagaa agcgaacccc agcaactacg
    15841 ctatggtcac cgtgagcgaa ggcgcgcaca tggtcggcgg caaggtcgtc gagtatggcg
    15901 agactgacgc ctacggccac cgcaagctgg gcggcatcgg cgaaatcacc ggcgaagcgc
    15961 tcaagaaact gaccggcgag aatatcatca cccagcgcct cgcctacctg atgcgctccg
    16021 gcgcgcccga tgcgctcgat ctgatggtgg gcacaaacta cgctcagttg gccgtcgacc
    16081 tgatcgtcgg tggttcaagc ggccgcatgg tggccctgcg tgacggcgct tatacccacg
    16141 tggccatgag cgttgtcacc agcggcgtca agcgcgtcga tgtagacgag ctctatgatg
    16201 cccagaacta tcgacccaag gtgcgccacg tcctcggcaa gccgatgttc ctgtattgat
    16261 tcgatcgttt cgctctgttc ttgcgaaggc gcgaaggtcc tcattggagg cctcgcgcct
    16321 ttttattttt attttgggcg gagatgagta aaccatgcgg gttgtctgat aacccgctga
    16381 gtcatggtcg atgtcacttg acgattcgcc cgttggctca ttagaatata gatatccttc
    16441 cacaagcaag tccgtccctg attgtcacag gtagcagctt ttcatagatt gccaatgtat
    16501 cgtttccatc gttcacccct atttttattc atcgtcattc ttgctctctc cgcgctcgcc
    16561 tgccaggctc tttcaatcga tccgtccgcc acgactcccc cggcacccag ccaacaggag
    16621 ttggtcgatc aggctgtggc cacggtgatg gctcaaattc cccaggcctc ggccttgccg
    16681 ccggtgacca cgagtcaatc ggcgccgatc agcaccgaca ttgagagctc gctgatcgag
    16741 ctttacaagc gcgtcaaccc ggccgtcgtc catatctacg tgatcgatga gcaatcattt
    16801 gtgctgggca cgggcagcgg cttcgtctat gacgaattgg gtcatatcgt caccaataat
    16861 catgtcgtga ccggcgcaag cggccttgag gtggtattcc ccgatggcac gcgccgatca
    16921 gctgaggttg tcggcaccga tgtcgatagc gacctggccg tcatcagggt agatgacctg
    16981 ccggcgggcg tgttgcccat tccgctgggc gattccgaca atgctcaggt aggccagttt
    17041 gtcgtggcta tcggcaaccc gttcgacgag gcaggctcgc tttcactggg catcatcagc
    17101 gggctgggtc gatcgctgtc ctctgaccgg atcgccgaag gtggcggccg ctattcgctg
    17161 ccccaggtca ttcagaccga cgcggccatc aaccccggca attcaggcgg gccgctgctt
    17221 aatctggccg gcgaggtggt gggtgtcaat agcgccattc gcaccagcac cggcacgaat
    17281 tccggcgtcg gcttctctat tcccgtcaac gccgtgcgac gcgtggcccc cgcgctcatc
    17341 gccgagggag tctatcatta cccctacatc ggcatacgca tgacctcgct cgatctggcc
    17401 tcgcaacagg cgatggggct gccacaagcc gcaggggcct atgtaattga catgacaccc
    17461 aacagcccgg ctcaaagagc gggcctgatt gccagtgggt ttaacgacac cgttggcaca
    17521 cttcccggcg gtgacctgat catcgctgtg gatgggcggc cgatttcaag tcccg
//
LOCUS       DAOMQG010000031        16724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1510_length_16724_cov_11.874378, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000031 DAOMQG010000000
VERSION     DAOMQG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1510_length_16724_cov_11.874378"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1810)
                     /locus_tag="PLC06_03395"
     CDS             complement(347..1810)
                     /locus_tag="PLC06_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89519.1"
                     /translation="MSLALIVGCSRANGNQGANPTDTATAEIATATVRPEPSPTATAT
                     PDPTPIPAGVTISDQPLDESGVLIADEVTLPSPGWLVIYRLVDGKTEDVIGYQPLAAG
                     VYEQVQITVDAKAATDDLMAGVHVDAGTEGVFEFPGEDVPYPGEPETTFAVELLIPVP
                     QVEVADQSVAEDGVVTLAALEILQPTWVAIHTDDDGKIGPVIGSRLFEPGTYENATIR
                     IDWRRATPRLHVVMHVDEGKANVFEFPDGDMPILMRGKPLITSFEATYPPEVRVYDQP
                     VIDGTVTIERAISDGPGYVAIYNESEGQPGFIIGTQALKDGLNERITVSLLRSAITTQ
                     LFARLHEDTEPGDAFNFPGQDPPVLYNNRLPNPTAFRTDVGAHVFISDQRLSADNTVS
                     IATIVSPVASWAVIYDDVDGQPGDLLGRTWAPAGVNRDVAVELGEAVGPGTLHLILYE
                     DLGTSEEFEVPGADIELRNDDGRPVRLPFELLPPASN"
     gene            complement(1953..4403)
                     /locus_tag="PLC06_03400"
     CDS             complement(1953..4403)
                     /locus_tag="PLC06_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRO89520.1"
                     /translation="MSLTQFPPADDSLKPRVDSRFYLALVGGVGWLVGVWLGSMSDLS
                     SYVWGLLFFPFAIGSVVYWKRGRLGLSLAFCAALALGGWRYMSSRLPFQPAYLHYYNG
                     GKDIEIWGVVVEEPAVYDTRVQLRVAAESLVVDGQAQPVSGIVQIDTRRFPEIPYGAQ
                     LHMVGDLSDPIALGDPGYAAYLERQGVLSVMESPRVEVTGRDGGNRLMRTLLAVKQRS
                     RDVITTSLPEPHASLLMGILLGDSRGMPRELKEDFRETGMTHVIAISGFNIAMIIALL
                     DYLAAPFLPRRTGALMIMIFIGLYAMLVGAGASVVRASIMGISYLVGLRLLGRPTLAI
                     AGLFTAAFFMTLANPDTLWDIGFQLSFAATLGLMLYAGRWERWFARGVVPALDPQARG
                     RVNRVASDLLFVTLAAQVLTLPLILYHFGRLSLASLPANILVLPAQGGVMIGGGLTLI
                     AGLIAPGLGQLVGLIAWLFLHYTISVIRILAQIPYASVPLPLTSSGLLAAYLAIGAIT
                     ILATQARTKSEQQTPRIDTRRQKTIVAIGLLLVSLALIGWIIGQSDNRLHVAFLDVGQ
                     GDAIFIQTPNGRQLLIDGGRYPSETLDQLGRQLPFWDRSIDIVMATHPDADHVAGLVD
                     VVERYNVALLITNGADEDSDSDYAALLAAADEQGTDVHIAQKGESIRLDEGVLIDILH
                     SGDVGKPDSRNDASIVARLSYGALSVLLTGDAEESSEMRMLQEGTPIQSAVLKAGHHG
                     ANTSSSAEFLKAVSPQIVVISVGRDNSYGHPHPDMLARAAGVGATVLRTDELGTLELE
                     SDGSQMWWSAEHNETAVP"
     gene            4665..5378
                     /locus_tag="PLC06_03405"
     CDS             4665..5378
                     /locus_tag="PLC06_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89521.1"
                     /translation="MASDSDASSYRILVLDDNYSMQRLVSTALQKAGFEVSTVSSAEE
                     GLENIQRYGLPHLALVDIHMPYGMNGLEFCEAILAFSDLPIIMLTAVDEDETIIQAID
                     QFAEDYITKPFNPAEMVARVRRVLRRIGDFAYTLSTHTVVDQYLTVSFPYQKAFVNGS
                     EVPLTPTETKLLYILMRHAGQTVTTDFILRRLWPMETAYEDRLRVYVHRLRRKIEHSP
                     NQPQYIVSQRGVGYQFAPN"
     gene            complement(5384..5881)
                     /locus_tag="PLC06_03410"
     CDS             complement(5384..5881)
                     /locus_tag="PLC06_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89522.1"
                     /translation="MDIQHLVDNLEQALNESTRVPLSAYLIVNEEKVYSLLDQMRVAV
                     PEEIKRANRIEAEKDRILAQAKEEAERIRDLARQEAGELVKRDAIVNAAQHRAENILE
                     RARRDADALRQDADVYIMDVLNRLEEDLTRTLKVVQNGLHKVESDHQAAQQAMSEATA
                     PNPAR"
     gene            complement(5892..6419)
                     /gene="coaD"
                     /locus_tag="PLC06_03415"
     CDS             complement(5892..6419)
                     /gene="coaD"
                     /locus_tag="PLC06_03415"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRO89523.1"
                     /translation="MKRAIYPGTFDPVHYGHLNLILRAANLFEELVVGVYDHSRPTKS
                     VLFSIEERVGMILEALENPGNIVVVPFTGLLVDFARKSDARVIVRGLRVFSDFEFEFR
                     SALANQRLAPEIETVSLMTREENSFVSGSTVREIASLGGDITSMVPPNVAAFLYERFG
                     SAEGASFSGNLPLRD"
     gene            complement(6471..9062)
                     /gene="recG"
                     /locus_tag="PLC06_03420"
     CDS             complement(6471..9062)
                     /gene="recG"
                     /locus_tag="PLC06_03420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRO89524.1"
                     /translation="MDRALKRLERVLELEQQQGYQNKAVVGGIRQFAVFWISQAQEEA
                     TSEADLALAEQISQVLMDYNQLPGTQARAKAIESLFQNLERRKAHKPVAETPSAVPPI
                     ERPKAKPRDEGVPIKAVAPASVPKPLASTEKEETPAKDEDYEAELEEEGFEEAQPEPE
                     EEHEADIPHVAPDPKGLNKSVTTIKGIGQKFAEKLNRLGAGTILDLLYLFPRRYDDYT
                     LLKPINKLQYGEQVTVIGTIWQTKVRRSRTNQAIIESIVNDGTGSIQVTWFNQPWLAD
                     QLRAGLQIALSGKIDMFLGRLMFNSPEWEPVEMESLRTRRIVPVYPLTEGLTATKMRE
                     LMQRVVKEWAARVPDPLPVEIRKRRRLYSLPRAIEQMHFPDSHESMQRARQRLAYDEL
                     FQLQLGVQRQRRDWQTHNGMALSIESSQFDTFMKALPFELTGAQRRVIDEIRADLAQS
                     RPMNRLLQGDVGSGKTVVAAAAMVSAAWAGAQSALMAPTEILAEQHYRGLHALLEPMG
                     FNVALLTGSTPASERQAIYEGLSDGSIQIAIGTHALIQPVVAFHRLGVAIIDEQHRFG
                     VDQRAALREKGPVGDDGAISPHLLVMSATPIPRTLALSLYGDLDMSILDEMPPGRQEI
                     KTRWLRASERERAYNFIRRQTAEGRQAFIIYPLVEESDMIDARAAVEESERLSREVFP
                     DRRVGLVHGRLRSDEKDAAMRAFVQHETDMLVATSVIEVGVDVPNSTVIVIEGADRFG
                     LAQLHQFRGRVGRGQHQSYCILIAEDVSAEAEQRLSALEATNDGFILAERDLELRGPG
                     EFFGRRQSGLPELRLASLLHDGEVLTMAQEDAAAIFAEDPNLELPEHQYLRERVEEFW
                     ETAADVS"
     gene            complement(9142..10809)
                     /locus_tag="PLC06_03425"
     CDS             complement(9142..10809)
                     /locus_tag="PLC06_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRO89525.1"
                     /translation="MTAPVTAGGKWLHCTGPQLRKMARAGLVWLERNRDHVNSLNVFP
                     VPDGDTGTNMLLTMRSAYARIEAGEETHVGKVAGQLAQGALMGARGNSGVILSQIWRG
                     LAAGLSSVETFTTADLAQAFQMASDTAYKGVMRPVEGTILTVIREGAAEAADAARKSE
                     DLRFMLERILERCQQALERTPDLLPILRQAGVVDSGGQGLVYILEGMMRYAQGELSLD
                     EQAEARAATENISAQALAVPEGGSLEFPYDVQFILTGRNLNVSQVRARIDAMGDSTVV
                     VGDSSTIKVHIHVKDPGEPLSYGISLGHVSDVVVENMQEQMEALVHGDPTAPVESVVT
                     VMPDQVAVVAVAAGRGLINVFHSLGAAYVVNGGQTNNPSTEEIFQAIEKLSTDKVVIL
                     PNNKNIFLAAEAACDLSAKDVAVVHSRTIPQGIAALMALRPEREVETNAETMEQALKL
                     VKTGEITRATRTVEIDGVDVKEGEIIALADGRLCSSGPDERAVLDELLEQLEVAEAGI
                     VSLYFGADISVGQAAETADHIRALFPDVEVELVEGGQAHYHYILGVE"
     gene            complement(10860..11216)
                     /locus_tag="PLC06_03430"
     CDS             complement(10860..11216)
                     /locus_tag="PLC06_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRO89526.1"
                     /translation="MPVQEESKGKIDISSATIATITNQAVNQCYGVVGMANKNLVNGI
                     AQLLSRENRRGVDVKVDGDKIVIDVYVIVQYGVRIRVVAESIQNTVKFHVEKALGLPV
                     HAVNVYVQGLRMSDQQ"
     gene            11373..11552
                     /gene="rpmB"
                     /locus_tag="PLC06_03435"
     CDS             11373..11552
                     /gene="rpmB"
                     /locus_tag="PLC06_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRO89527.1"
                     /translation="MAKCEVCGKGPMSGHNVSFSQRKTKRQFLPNIQKTTVVQGGRRV
                     RLHVCTRCLKTMAKV"
     gene            complement(11726..12667)
                     /gene="mvk"
                     /locus_tag="PLC06_03440"
     CDS             complement(11726..12667)
                     /gene="mvk"
                     /locus_tag="PLC06_03440"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HRO89528.1"
                     /translation="MFTATAPGKVILFGEHAVVYGHPALAAPVVQLRARATVEETGRS
                     DVALIAPDLNRSALLSKVHAHNPLAAVVRVVEQYCNCSLSDGYSLSVTSAIPIASGLG
                     SGAAISVAVIRALAQYMGREDAISREEVSRLAYEVERLHHGTPSGVDNTVIAFERPVY
                     FVRREPEPLIETFVTAQPLRLVIGDTGIRSATKDVVGDVRRQWSADAERFETIFDACG
                     RIAVAGREALAAGDLPTVGALMNENHEWLARMTVSSDALDHLVAAARGAGALGAKLSG
                     AGRGGNMIALVTAETEAVVAEQLLAAGAARIFTSDVG"
     gene            complement(12673..13545)
                     /locus_tag="PLC06_03445"
     CDS             complement(12673..13545)
                     /locus_tag="PLC06_03445"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRO89529.1"
                     /translation="MLSGLIKSMRPRQWSKNAFVFVALLFDRKLFDPVSVAAVLGAFI
                     LLCLMSSAVYLMNDLADIESDRQHPTKKDRPLPSGRLNPRVAMVASFLLAAVSLVAGY
                     FLSYKLAILLLLYLISQIAYTFKLKHVVLLDVLTIASGFILRIAAGVAVIDVERFSPW
                     LYVFGGFLALFMALGKRRAELTLLGGGAGSHRAILDDYSLDLIDRLQSIVTTSAIVAY
                     SLYTFLAEGLPENHAMMLTIPFVLYAIFRYLYLIHVRSEGGAPEEILLRDKPLQITLV
                     LYGIMVIVALYLIK"
     gene            complement(13696..14796)
                     /locus_tag="PLC06_03450"
     CDS             complement(13696..14796)
                     /locus_tag="PLC06_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89530.1"
                     /translation="MNPQLSAESANKVVGRRAFVRPLSLTLGLAVVAAVIFAWAAPAR
                     SSTIPTISISSVVTDQTVTVQANNFPANQNFVVTMGPMGTQGVNGTVVASINSGVGGS
                     FSATYNIPDGLKGSERVAVRLQTGHTYPYYAYNWFWNNLAGVPGGNPGSVAVPTFSIV
                     SVEQGKTVTIETKDFPSNQAFTITMGPMGTQGVNGAVVGTLNSGVGGVLKATFNIPAQ
                     LATAYQIAIRAQTAHAAPFFAYNWFYNNTVNAGAGGGTVTPPPSGPVYTGTPTFTVCA
                     VARDTTVTVKTKDFPPNQAFTVTMGAYGTQGIGGYQVGTLNSGTGGALTLTFNVPPQL
                     AGSGRIAIRMQTAHAYPFYAYNWFFNNTATVC"
     gene            complement(15036..16217)
                     /locus_tag="PLC06_03455"
     CDS             complement(15036..16217)
                     /locus_tag="PLC06_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRO89531.1"
                     /translation="MMLDKAEKLSDELVRLRRTIHANPELSFQEFQTAALVTDTLSEI
                     GGYTIHTQVGKTGVMAELGDSGPIIAIRADMDALPIIEANDVPYASKNPGVMHACGHD
                     AHTAILLGVAHLLRQSYAEEQWQGRVRLLFQPSEEASDENNISGATAMIMDKALEGVD
                     AVIALHVISDRPSGEFMFQDGPSLAAVDSFDAWVYGDGAHGAYPHAGSDPIFMLAPIL
                     TAIYAIPSRRINPLYPAVISIGQITGGATTNVIPNEVMLRGTMRSMLPEVRQQLWHEL
                     EAALRMSESMGGGYKLNIAKGYPAMVNDPGANDWMRDVTRELLGDGAVVPSQFGMGGE
                     DFAYMTQKAKGAMFMLGAATPDGQARHHHTSIFDIDERVLAPGAAVLAETARRFVTGA
                     L"
     gene            complement(16287..>16724)
                     /locus_tag="PLC06_03460"
     CDS             complement(16287..>16724)
                     /locus_tag="PLC06_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRO89532.1"
                     /translation="AGAGDLFLASREGKGIRFMERQVPVRGCLGIRLDPTDEAVAVAA
                     VGPEDGVFLLGEDGQGTVRLMSGFSANKAPGAGGKVAMKSDRVVGAARVGVDDELFVI
                     SRLGKLIRFAAEEIPFKEGVVQGVACMSLRADEAVAIAVAPRD"
BASE COUNT         3720 a         5067 c         4512 g         3425 t
ORIGIN      
        1 ttcgcgtgtc tgcgcagtat tggaagcgag agacatcgag gcaactcctt gggcatctgg
       61 gatgtcacgt gatcaaataa catgaatgtt cgcgcaatgc gcgcgtcggg cggtatgtac
      121 gttttttgga tgtggctatg gttagctatt tgagtgctgg tagccgttac cgccatgagg
      181 gatacattgc tcactcattc aatgaaatag tgccacacct gagcaaatga gaacatgaac
      241 aggacgtgaa atgttggtac ggaatacgaa aaagagagat gcggactgag ccatatccgg
      301 gttatttgtg tttgcttgtc cctctgagtg aggaagaaag acgcatctaa tttgaagcag
      361 gtggcagcag ctcaaacggc aagcgaaccg gtctgccatc atcgtttcta agttcgatat
      421 cggcgccggg cacttcgaac tcttccgagg ttcccaaatc ttcatacagg atcaggtgga
      481 gggttcccgg cccaacagcc tcaccaagtt caacggccac atcccgattc acacccgcgg
      541 gcgcccaggt tcgccccagc aggtcccccg gctggccatc cacatcgtca tagatcaccg
      601 cccaggacgc aaccgggctt acgattgtgg caatagatac ggtgttatcg gcgctcaggc
      661 gctggtcgga tatgaacacg tgcgcgccga catcggtgcg aaaggcggtc gggttcggca
      721 ggcgattatt atagaggaca ggcggatcct ggcctggaaa gttaaaagca tcgcccggct
      781 cggtatcctc atgcaggcgc gcaaataact gggtcgtgat ggccgatctc aacaagctga
      841 cggtaattcg ctcattcaga ccgtctttca acgcctgggt accgatgata aagccgggct
      901 gaccctcgga ttcattgtaa atcgctacat atcccggccc atcgctgatg gcccgctcga
      961 tcgtgacagt tccatcgatc acaggctgat catagacgcg aacttcaggt ggatatgtcg
     1021 cctcaaatga ggtaatgagc ggcttgccac gcatcagtat cggcatatcc ccgtcgggga
     1081 attcaaagac attggccttt ccctcgtcca catgcatgac cacatgcagg cggggcgtcg
     1141 cccgtcgcca atcgatcctg attgtggcat tctcataggt cccaggctcg aacagccggc
     1201 ttccgatgac ggggccgatc ttgccatcat cgtcggtatg gatggcaacc catgtcggct
     1261 gcaggatttc cagagcggcc agcgtcacca cgccatcttc agcgacagat tgatcggcaa
     1321 cctcaacctg agggaccggt attagcagtt ccaccgcaaa agtcgtttcc ggctcgcccg
     1381 gataaggtac atcctcgccc ggaaattcga agactccctc agttccagca tccacatgaa
     1441 cgccggccat caaatcgtcg gttgctgctt tggcgtcaac tgttatctgt acttgctcgt
     1501 agacgccggc ggccaagggt tggtaaccaa tcacatcctc cgtcttacca tccactaacc
     1561 gatagattac cagccagccg gggctgggca gggtcacttc atcagcaatc agtacgccgc
     1621 tttcatccag aggctgatca cttatcgtga cgccggcggg gatgggggtg gggtcgggtg
     1681 tagccgtggc cgttggggaa ggctcaggcc ggaccgtcgc tgttgcaatt tcagccgtcg
     1741 ccgtatccgt gggatttgcg ccctgattcc cattggcccg ggaacatccg acgatgagcg
     1801 ctaaggacat gaacagggcc aaaaccctga agcgttgaga gttcaagtta agtcgtcacc
     1861 cttttttcta tcgaccgacg attgtagcgt tatatcatcg gactgccact cctctatgag
     1921 aatgctcagc agagattcca tgaccagggt agtcacggaa cggccgtttc gttgtgttcg
     1981 gccgaccacc acatttgcga gccgtcggat tccagctcca gcgtgcccag ttcatccgtg
     2041 cgcagcacag tcgcccccac ccccgctgcg cgggcaagca tgtccggatg aggatggcca
     2101 tatgaattat cacggccgac cgagatgacc acaatctgcg gcgagaccgc cttcaggaac
     2161 tcagcactac tggatgtatt ggcgccatga tggcccgcct tcaggacggc cgactgaatc
     2221 ggcgtccctt cctggagcat gcgcatctcg gacgattcct ccgcgtcacc ggtcaataat
     2281 accgataagg ctccgtagct caatcgggca acaattgacg catcattacg gctatcgggt
     2341 ttgcccacgt cgcccgaatg aagaatatcg atcaggacgc cctcatccag ccggatgctt
     2401 tctccctttt gagcaatatg gacatcggta ccttgctcat cggcagccgc cagcagtgcg
     2461 gcataatccg agtcgctatc ttcatctgcg ccgttggtga tcaataatgc gacattgtat
     2521 cgctcgacga catcgaccag cccggccacg tggtcggcat ccggatgagt cgccatcacg
     2581 atgtcaatgg atcgatccca gaatggcagt tgccgcccca gttggtcgag cgtctcactg
     2641 ggataacgac cgccatcaat caacagctgt cggccattgg gggtctgaat gaatatagca
     2701 tcgccctggc caacatccag aaacgccacg tgtaaacgat tatcagactg cccaatgatc
     2761 cagccgatca aggccaaact cacgaggagc agcccaatgg cgacgatagt cttttgtcgc
     2821 ctggtatcta tgcgaggcgt ctgctgctcc gatttcgttc gcgcttgggt ggccaaaatc
     2881 gtaatcgctc caatcgccag ataagccgcc aacaggccgg atgatgtgag cggcagagga
     2941 acagatgcat aaggtatctg cgccagtatg cggatgacgc taatagtgta gtggaggaac
     3001 agccaggcaa ttaatccaac cagttgcccc agacctggcg ctatcagacc ggctatcaag
     3061 gtcaaccctc caccaatcat caccccgccc tgggcgggca gaaccaggat attcgccggc
     3121 aagctggcca aagatagccg cccgaagtga tagaggatca acggcaacgt caacacctgg
     3181 gccgccagcg tgacaaacag gagatcggac gcgacacgat tgacacggcc gcgcgcctgg
     3241 ggatcgagcg ccgggacgac gcctcgggca aaccaccgct cccatcgccc ggcatagagc
     3301 atcagcccca atgtagccgc aaagctcaat tgaaaaccaa tatcccacag ggtatcgggg
     3361 ttagccagag tcatgaagaa tgcggccgtg aaaaggcccg ctatcgccaa agtcggccgc
     3421 cccaggagcc gcaggccgac aagatagctg atgcccataa tcgatgcgcg cacaaccgac
     3481 gcgcccgcgc caacgagcat ggcatacagg ccaataaaaa tcatgatcat cagcgccccg
     3541 gttcggcgtg gaaggaaggg agccgccaaa tagtcgagca gggcgatgat catggcaata
     3601 ttgaagccgg agatggcgat aacgtgggtc atcccggtct cgcggaaatc ctctttcaac
     3661 tcacgcggca ttccccgact gtcgcccaga aggatgccca tcaacagcga ggcatggggt
     3721 tcgggcaacg atgtagtgat gacgtcgcga ctccgttgtt tcacggcgag taacgtgcgc
     3781 attaaacgat taccgccatc cctgcccgtt acttccacac ggggggattc catgacgctc
     3841 agcactcctt ggcgctccag ataggcggcg taaccgggat cgcctaatgc tatcgggtcc
     3901 gagagatcgc ccaccatatg gagttgcgcg ccatagggga tttcgggaaa gcgtctggtg
     3961 tcgatttgga cgatacctga gaccggctgg gcctgaccat caacaacaag gctctcggcc
     4021 gctaccctga gttgcacgcg cgtatcatac acggcaggtt cttcaacgac gacaccccat
     4081 atctcgatat ctttaccgcc gttgtaatag tggagatagg caggctggaa aggaagacgg
     4141 ctactcatat agcgccaccc gcccagcgcc aacgccgcgc agaaagccag agagagaccc
     4201 aagcggccgc gcttccagta gacgaccgat ccgatggcga acggaaaaaa caagaggccc
     4261 cagacatatg agctgaggtc actcatcgac cctagccaaa ccccgaccag ccaacccacc
     4321 ccaccaacca gcgccagata aaagcgggaa tcgacgcgag gtttaaggct atcatccgcc
     4381 ggtggaaatt gcgtcaatga catgaagttc tgccggaagc cgtgcttcta acacatccag
     4441 ctcaaatttt acaatccaaa acgcaaacag cctatgacga cgaaaatatg tcgtgaaaag
     4501 aacttgaggc aaattaatct tctgttagaa taggtccggg gtcggaatta gggacagaaa
     4561 ccatcgttgt cgcaagatcg tgtttggaag atggtgtcag accggtggaa cgtattcacc
     4621 ccaagcgaca ctaatcagcg agggagatcg tcaggatttt gggcatggct tctgactctg
     4681 atgcaagcag ctaccgtatt ctcgtccttg acgataatta ctccatgcag cgcctggtct
     4741 ccacggcgct gcaaaaggcg ggctttgagg tatcaaccgt ttcctcggcc gaagaaggcc
     4801 ttgagaatat ccaacgatac ggcttgcccc acctcgccct ggtcgacatc catatgcctt
     4861 atgggatgaa tgggctggaa ttttgtgagg cgatcctggc cttttcggat ctgccgatca
     4921 tcatgctgac ggccgtcgac gaagacgaga cgatcatcca ggccattgat cagttcgccg
     4981 aggattacat caccaagccg ttcaatccgg cggaaatggt ggctcgcgtc cgtcgtgttt
     5041 tacggcggat aggggacttc gcctacaccc tgagcacaca cacagtagtc gatcaatatc
     5101 tcacggttag cttcccctat cagaaggcat ttgtgaatgg cagcgaggtc ccactgaccc
     5161 cgacagaaac gaagctcctg tacattctca tgcgccacgc cggccaaacg gtgacgaccg
     5221 acttcatctt gcgacggttg tggcccatgg agacggctta cgaagacaga ttacgcgtgt
     5281 atgttcatcg gttacggcgc aaaatcgagc actctccgaa ccaaccccaa tacatcgtct
     5341 cgcaaagagg cgtcgggtat cagttcgcac cgaattaatt cgtttagcgc gcagggtttg
     5401 gggcggtagc ctctgacatc gcttgctggg cggcctgatg gtccgattcg accttatgca
     5461 ggccattctg aaccactttc aacgtgcggg tcagatcctc ctccagtcga ttaagcacat
     5521 ccatgatgta aacatccgcg tcctgccgaa gcgcgtcggc gtcgcggcgc gcccgctcga
     5581 gaatattctc ggcacgatgc tgggcggcat taacgatggc gtcacgcttg accagctccc
     5641 ccgcttcctg tcgcgcgagg tcacggatgc gttcggcctc ttccttggcc tgggccagga
     5701 tgcgatcttt ctcggcctcg atccgattgg cgcgcttgat ctcctccggc acggcaacac
     5761 gcatctgatc gagcaggcta tacaccttct cttcattgac gatcagataa gcactcagcg
     5821 gcactcgagt gctctcattc agggcttgct caagattatc gacaagatgt tgaatgtcca
     5881 tgcgtgttca cctaatcgcg aagcggtaaa ttccccgaga atgatgcgcc ctcggccgaa
     5941 ccaaatcgct cgtagaggaa agccgccacg ttggggggaa ccatgctggt gatgtcccca
     6001 ccaagtgacg cgatctcgcg caccgtgctg ccactgacaa aagagttttc ctcgcgggtc
     6061 atcaggctga ccgtttcaat ctccggggcc aatcgctggt ttgccagggc cgaccggaac
     6121 tcgaattcaa aatcggagaa cacccgtaag ccacgaacga taacccgagc gtccgatttt
     6181 cgagcgaaat ccaccaacag accggtaaag ggcacgacaa cgatattccc tgggttctcc
     6241 agagcttcca aaatcatgcc tacccgttct tcaatagaga ataacaccga cttcgttgga
     6301 cggctatgat cgtaaacgcc aacgaccagt tcttcaaaca gattggccgc gcgcaggatc
     6361 aagttcagat gaccataatg aacgggatca aaagtacccg gatagatggc tcgtttgatc
     6421 aaagttccct ctcctcaatt ccgttcatcg tcccaaatcc gggattatcc ctaactgaca
     6481 tcggccgccg tttcccagaa ctcttccacg cgctcgcgca gatattgatg ctccggcagt
     6541 tccaggttgg gatcttcggc gaagatagcg gcggcatctt cctgggccat ggtaagcact
     6601 tcgccatcat gcaacaaaga ggccaggcgc aactccggca ggccactctg gcgacggccg
     6661 aagaactcac cggggccgcg caactccagg tcacgctcgg ccagaataaa accatcgttt
     6721 gtcgcctcca gcgccgaaag acgctgctcg gcctcggccg acacatcctc agcgatgaga
     6781 atgcaatagg attgatgctg cccacgcccg acccggccgc ggaactggtg caactgggcc
     6841 aggccaaacc ggtctgcccc ctcgatgacg ataaccgtgc tgttgggcac atcgacgcca
     6901 acctcgatga ccgatgtcgc caccagcata tccgtttcat gctggacaaa ggcacgcatc
     6961 gcggcatcct tctcgtccga gcgcaatcgg ccgtgcacga ggcccacacg tcgatccggg
     7021 aagacctcgc ggctgagtcg ctccgactcc tccaccgcgg ctcgcgcatc aatcatgtcc
     7081 gattcttcaa ccagcgggta gatgatgaat gcctggcgac cttcggccgt ctggcgacga
     7141 atgaaattat aggcgcgttc gcgctccgaa gcccgtagcc agcgcgtctt gatctcctga
     7201 cggccggggg gcatctcatc aaggatcgac atatccaggt cgccgtagag tgagagggcc
     7261 agcgttcgtg gaatgggcgt ggccgacatc accagcaagt gggggctaat cgcgccgtca
     7321 tcacctacgg ggcctttctc gcgcagcgcg gcacgttgat ccacgccgaa acgatgctgc
     7381 tcgtcgatga tggcaacgcc gagccgatgg aaagcaacca ccggctgaat cagggcatgg
     7441 gttccgattg caatctggat cgatccatca gacaaccctt cgtagatcgc ttgcctctcg
     7501 gacgccgggg tgctgccggt cagcagcgcg acgttgaacc ccatcggctc caacagtgcg
     7561 tgtaagccac ggtaatgctg ctcggccaga atttcggtgg gcgccatcaa cgcggattgc
     7621 gctccggccc atgcggccga caccatcgct gctgcggcca caacggtctt gcccgaaccc
     7681 acatcaccct ggagcaagcg gttcatgggc cggctttggg ccagatcggc gcgaatttca
     7741 tcaatgactc gtcgctgcgc gccggttaac tcaaacggca acgccttcat gaacgtatcg
     7801 aactgcgagg attcaataga tagcgccatc ccgttatgag tctgccaatc gcgccgctgg
     7861 cgctggacgc caagttgcag ttggaacagc tcatcatagg ccaaacgctg ccgggcacgc
     7921 tgcatcgatt catgactatc ggggaaatgc atctgttcga tggcgcgggg caacgaatag
     7981 agacggcgac gtttgcgaat ctcgaccggc aacggatcgg gcacgcgagc cgcccactcc
     8041 ttcacgacgc gttgcatcag ctcgcgcatt ttggtcgcgg tcagtccctc ggtcaatgga
     8101 tatacaggaa caatgcgtcg ggttcgcagc gactccattt ccacaggctc ccactccggc
     8161 gaattaaaca tcagtcgacc gaggaacatg tcgatcttgc cgctcaacgc gatctgtaat
     8221 ccggcccgca gttggtccgc caaccacggc tggttaaacc atgtcacctg tatagagccg
     8281 gtgccatcgt tgacgattga ctcgatgatg gcctgattcg tgcgcgagcg tcgaaccttg
     8341 gtttgccaga tagtgccaat gaccgtgact tgttcgccat attgcaactt gttgatcggc
     8401 ttgagcagcg tgtaatcatc gtaacgacga ggaaacagat agagcaggtc aaggattgtc
     8461 ccggctccca acctgttcaa cttctccgca aacttttgtc cgatgccctt gatcgtggtg
     8521 accgacttgt tcagcccctt gggatcagga gctacatgcg gaatgtcggc ttcatgctct
     8581 tcttcaggct cgggctgtgc ttcttcgaag ccctcttcct caagttccgc ttcgtaatcc
     8641 tcatcttttg ccggcgtttc ttctttttct gttgacgcta gtggctttgg cacagaggcc
     8701 ggcgcgacgg cttttattgg cacgccctca tcacgcggct tcgctttcgg tcgctctatt
     8761 ggtggaacag cagatggggt ttcggcgaca ggtttgtgcg ccttacggcg ctccaggttt
     8821 tggaacaacg attcgatggc cttggcccgc gcctgcgttc ccggcaactg gttataatcc
     8881 atcaacacct gcgagatctg ctcggccaaa gccaggtcag cctcgctggt cgcctcttcc
     8941 tgtgcctggg aaatccaaaa tacggcgaat tggcggattc cacccacgac cgccttattc
     9001 tgatatccct gttgttgctc aagttcgagc actcgctcga gtctctttag tgctcggtcc
     9061 atatgtacgg tcttcaaact cctgcgcgca gcctgctctt cccgaaccgg ccggcgcttc
     9121 ttcttgcctt atccctgtgg tttattcgac gcccaatata tagtgatagt gtgcctgacc
     9181 accctcgaca agttcgacct caacgtcggg aaaaagcgct cgaatatggt cggccgtctc
     9241 ggccgcctgc ccgaccgaaa tatcagcccc aaaataaagg ctgacaattc cggcctcagc
     9301 tacttcaagc tgttcgagca gctcatcaag caccgctctc tcgtccggtc ccgagctaca
     9361 caatcgacca tcagccaagg caataatctc gccttctttc acatcaacgc cgtcaatctc
     9421 aacggtgcgt gtagcccgcg tgatctcccc cgttttaacg agcttcaggg cttgctccat
     9481 cgtctcggcg ttggtctcta cctcgcgctc cggccgcagg gccatcaggg cggcaatgcc
     9541 ctgcgggatc gtgcggctat gaacgacggc cacatctttg gcgctcaggt cacatgccgc
     9601 ttcggccgcc aaaaagatat tcttgttgtt cggtaatatg acgaccttat cggtcgacag
     9661 tttctcaata gcctgaaaaa tctcttccgt gctggggtta ttggtttggc cgccattgac
     9721 cacataggcc gcgccaaggc tgtgaaacac gttgatcaac cccctgccgg cggcgacggc
     9781 cacaaccgcc acctgatcag gcatcaccgt cacgacagac tccaccggcg cagtcgggtc
     9841 gccatgaacc agcgcttcca tctgctcctg catgttctcg accacgacat cgctgacatg
     9901 acccaggctg atcccatagc tcaaaggctc gccgggatcc ttcacgtgga tatggacctt
     9961 gatggtcgag ctgtcaccaa cgacgaccgt cgagtccccc atcgcgtcga tgcgcgcgcg
    10021 tacctgcgat acattcagat tgcgccccgt caggatgaac tgcacatcat aagggaattc
    10081 cagtgagccg ccctcaggca cagccaacgc ttgagctgag atattctcgg tagcagctcg
    10141 cgcttcggct tgctcatcaa gcgacaattc gccctgggca taacgcatca tcccctccag
    10201 gatatagacc agcccctggc cacccgaatc gaccacgccg gcctgccgca ggatgggcag
    10261 cagatcaggt gtccgctcca gcgcctgctg gcagcgctcc aatatgcgct ccagcatgaa
    10321 gcgcaggtcc tcgctcttgc gggccgcgtc ggcagcctcg gccgctccct cgcggattac
    10381 ggtcaggatc gtaccctcca ccgggcgcat aacgcccttg taggccgtat cggatgccat
    10441 ttggaatgcc tgggcgaggt cggccgtggt aaacgtctcc acgctgctga ggccggccgc
    10501 caggccacgc caaatctggc tcaggatcac cccggaattg ccccgcgcac ccatgagcgc
    10561 gccctgcgcc agttgccccg caaccttgcc cacgtgggtt tcttcgccgg cttcgatgcg
    10621 cgcataggcc gagcgcatcg tcaataacat gttcgttccc gtgtcaccgt cgggaacggg
    10681 gaaaacgttg agcgaattga cgtggtcgcg atttcgttca agccatacca gcccggcgcg
    10741 agccatcttg cgcagttgcg ggccggtgca atgcagccac tttccaccgg ctgtaaccgg
    10801 tgctgtcatc gtttctaatg ccgtagtcaa agccttcaat tcctccgata aggcgcccgt
    10861 tattgttggt cgctcattcg tagcccctgc acatacacat tcaccgcgtg cacaggaagg
    10921 cccagcgcct tttcgacgtg aaattttacc gtgttctgga tgctttcggc gacaacgcga
    10981 attcgcacgc cgtattggac gatcacatat acatcgatta cgatcttgtc tccgtcaacc
    11041 ttcacatcca caccgcgacg attttcgcgc gacaagagct gcgcgatgcc gttgacgagg
    11101 ttcttgttgg ccattccaac cacaccgtaa cattgattga cagcctgatt ggttatcgtc
    11161 gcgatggtcg ccgaggaaat gtcgattttc cctttggact cctcttgaac tggcataaat
    11221 cgtctgctcc tgccctgacg agacgcgctt gcatgagccg cgcctctcgt ctataataaa
    11281 ggattgttct taataacgtt gaaacttgtg gcgaccgacc ggtcgccttt gatttggacg
    11341 ccgtcagcat gggcgtcggg taaggtgaaa ttatggcgaa atgtgaagta tgtggcaagg
    11401 ggccgatgag cggccacaat gtaagtttct cgcagcgcaa gacaaagcgg caattccttc
    11461 cgaatatcca gaagactact gtcgtgcagg gcggccgtcg cgtgcgtctt cacgtctgca
    11521 cccgttgcct taagacgatg gcgaaggtct gaatcgagcg acgatccgat tggcgcgggc
    11581 agattcctgt tgccgaaaat tttcttatac aaaacaacct cgccggcgta aatcgccggc
    11641 ttttgttttt ggacggatga tcgtccgctt gccgattagc aattctactc gataagggca
    11701 aaccaaccca cttcccccac tctctctaac ccacatcgct ggtgaatatg cgcgccgcgc
    11761 cggcggccag caactgctcg gcgacgaccg cttccgtttc ggccgtgacc agggcgatca
    11821 tgtttccccc gcgccccgcg ccactcagct tcgctcccag cgcgcctgcc ccgcgcgccg
    11881 cagccacgag atgatccaga gcatcggacg acacggtcat gcgcgccaac cattcatgat
    11941 tctcgttcat gagcgcgccg acagtcggca aatcccccgc ggcaagcgct tcccgaccgg
    12001 ccacggcaat gcgtccgcac gcatcgaaga tcgtctcaaa gcgctcggcg tcggccgacc
    12061 attgacgcct cacatcgccg acgacatctt tggtggcgct acgaatgccg gtatctccga
    12121 tgacgaggcg aagcggctgg gctgtgacaa aggtttcaat cagcggctcc ggctcgcgtc
    12181 gaacgaagta aaccggccgc tcaaaggcaa tcaccgtgtt gtctacgccg cttggcgtgc
    12241 catgatgaag ccgctcgact tcataggcca gtcgcgagac ctcctcgcgc gagatcgcgt
    12301 cctctcggcc catatattgc gccagggcgc gtatgaccgc caccgaaatc gccgcaccgc
    12361 tacccagtcc actggcgatg gggatggcgc tggtgacgct cagagaatag ccatcggaca
    12421 atgagcagtt gcaatactgt tcgaccacac ggacaacggc cgccagcggg ttatgggcgt
    12481 gaaccttcga caaaagagcg gagcgattca gatcgggcgc gatgagggcg acatcggagc
    12541 ggccggtttc ctcaactgtc gcccgcgccc gcaactgaac aacgggcgcg gccaaagcag
    12601 gatgaccata aacgaccgca tgctcgccaa acaggatgac tttccccggt gctgttgccg
    12661 taaacatcga tgctacttga tcagatagag cgcgacgatg accatgatcc cataaagaac
    12721 gagggttatc tgcaacggct tgtcgcgcag caaaatttct tccggcgcgc caccctcgct
    12781 ccgcacgtga atcaaataga gatagcgaaa tatggcgtag agcacaaacg ggatcgtcag
    12841 catcatcgcg tggttttccg gcaatccctc ggccaaaaat gtatagagac tataggccac
    12901 aatagcgctg gtggtaacga tactctgcaa tcgatcgatc aggtccaggc tataatcatc
    12961 gagaatagcg cggtggcttc cagccccacc gcccagcagc gtgagttcgg cccgccgctt
    13021 gcccaaagcc ataaagaggg cgagaaaccc gccgaagaca tatagccagg gcgaaaagcg
    13081 ctctacgtca attacagcga caccggcagc aatacgcaaa ataaaccctg atgcaatggt
    13141 cagcacatcg agcaggacga cgtgcttgag cttgaaggtg taggcaattt gcgaaatcag
    13201 gtaaagcaac aggaggatcg ccagcttgta tgagagaaaa tagcccgcca ccaggctgac
    13261 ggccgccagc aggaaagagg caaccatggc cacgcgcgga ttgagccgac ccgaaggaag
    13321 tgggcggtcc ttcttcgtgg ggtgctgccg atcgctctcg atgtcggcca gatcgttcat
    13381 caggtagacc gcggaggaca tcaggcatag caagatgaac gcacccagaa cagccgccac
    13441 gctgaccgga tcaaacaatt ttcgatcgaa cagcagcgcc acaaagacaa aggcattctt
    13501 cgaccactgt cgcggccgca tcgacttgat tagtccgctt aacacgattt actcccagga
    13561 cgcataaccc cttgtttcta tcacgaatgg aatctccagg ccaatccacg gaacgcctca
    13621 ataccccgac aataaaaaag gcctcccctt tgtggggaga gccttttctc tatcaatgag
    13681 taatcgcaat gtggcttagc aaacagttgc cgtgttgttg aagaaccagt tataggcata
    13741 gaatggatag gcatgggctg tctgcatgcg aatggcgatc cggcccgagc cggccagttg
    13801 cggcgggaca ttgaacgtta gcgtcagcgc gccgccggtg ccggaattaa gcgtgcccac
    13861 ctgatagccg ccgatgccct gcgtgccgta ggcgcccatc gtcaccgtga atgcctgatt
    13921 cggcgggaaa tccttggtct tgacggtaac cgttgtatcc cgagccaccg cgcagacggt
    13981 gaacgttgga gtacccgtgt agacgggtcc ggaaggcgga ggtgtcacgg tgccgccgcc
    14041 ggcgcccgcg ttaacggtat tattatagaa ccagttgtag gcgaagaacg gcgccgcgtg
    14101 ggctgtctgg gcgcgaatcg cgatctggta ggcagtcgct aattgggcgg ggatattgaa
    14161 cgtcgctttc aacacgccac cgacgccgga gttcagcgtg ccgactaccg cgccgttaac
    14221 gccctgtgtg cccatcggcc ccattgtgat agtgaatgcc tgattggagg gaaagtcttt
    14281 ggtttcgata gtcacggtct tgccctgctc cacactgacg atcgagaacg tggggacggc
    14341 tacgcttcca gggtttccgc ccgggacacc ggccagatta ttccagaacc agttgtaggc
    14401 ataataggga taggtatggc ctgtttgcaa gcgaacagca acgcgctccg aacccttcag
    14461 cccgtcgggg atgttgtacg tcgcgctgaa cgatccgccc acgccggagt tgatcgaggc
    14521 taccacagtg ccgttaacgc cctgcgtgcc catcggcccc atcgtgacga caaagttctg
    14581 atttgcgggg aaattattgg cctgcaccgt gacggtctga tctgtgacaa cgctggaaat
    14641 gctgatggtg gggatggtgc tggaacgtgc cggggcggcc caggcaaaga tcaccgcggc
    14701 cacgacggca agacccagcg tcaacgacaa cgggcgaacg aacgcacggc gtccaacaac
    14761 tttgtttgcg ctttctgctg acaactgcgg attcatttga tacctcctga gtatgtacct
    14821 gttcgctctg tatggagatg atggcctcga gactactgga cgacatccgg cggatcataa
    14881 tcataccaac catcacgctc caatgatcca ctcagtctta acagtataca taatcacaaa
    14941 atagatatct atagaacgca agttctttat tgtcataatc cgccggaatt tgcccgaaaa
    15001 ttccgccgca acaagacccc gacaaagaaa atgacctata aagctcccgt gacgaagcgc
    15061 cgggcagtct ccgccaacac ggccgcgccg ggagccagta ccctttcatc gatatcaaat
    15121 atggaggtat gatgatggcg agcctggccg tcaggtgtgg ctgcgcccag catgaacatc
    15181 gcccctttcg ctttttgcgt catataggca aaatcctcgc cccccatacc aaactgggaa
    15241 ggcacaaccg cgccatcgcc caacaattcg cgcgtcacat cccgcatcca gtcattggct
    15301 ccgggatcgt tgaccatggc cgggtatcct ttggcgatat tgagcttgta gccaccaccc
    15361 atcgactcgc tcatccgcaa ggcagcctcc agctcgtgcc acaactgctg gcgaacctcc
    15421 ggcagcatgg agcgcatcgt cccccgcagc atcacttcat tcggaatcac attggtcgtc
    15481 gctccgccgg taatctggcc gatactaatg acggctggat acaatggatt gatacgccgc
    15541 gaagggatgg cgtagatcgc ggtcagtatg ggggccagca taaagatcgg atcgctgcct
    15601 gcatggggat atgccccgtg tgcgccgtcg ccatagaccc aggcgtcgaa cgagtcgacg
    15661 gcggccaggc tagggccatc ctggaacatg aactccccgg acggcctgtc cgaaatcaca
    15721 tgtaaagcga tcaccgcatc gacgccttca agcgccttat ccatgatcat cgccgtcgcg
    15781 ccactgatat tattctcatc ggacgcttcc tccgatggct ggaaaaggag ccgaacgcga
    15841 ccctgccact gctcctcagc gtaactttgg cgcaataaat gggcgacgcc cagcagaatc
    15901 gccgtgtgcg cgtcgtggcc gcaagcatgc ataacgccgg ggttcttcga cgcataagga
    15961 acatcattgg cctcgatgat cgggagggcg tccatatcgg cgcgaatggc gatgatcggg
    16021 ccggaatcac ccaactcggc catgacacct gtcttgccta cctgagtatg aatcgtatag
    16081 ccgcctatct ccgacaacgt atcggtcacc agcgcggccg tttgaaattc ctgaaagctc
    16141 aactccgggt tggcgtggat cgtccgccgc aaccggacaa gctcgtcgct taacttttcg
    16201 gctttatcca acatcatagg ttttctcctg ctgaagagcg caaccgaagt tgctattaaa
    16261 gggacaagaa cgcgctcgca agcgtatcaa tctcgcgggg cgacagcgat cgccaccgct
    16321 tcgtctgcgc gaagggacat gcaggccact ccctgaacaa ccccttcctt gaacggaatt
    16381 tcctcggccg cgaaacgaat cagtttcccc agccgggaaa tgacaaataa ctcatcatcc
    16441 acgccaacac gggccgcgcc caccacgcgg tcgctcttca tggcgacttt gccgcccgcg
    16501 cccggcgctt tattggccga gaatccgctc atcagccgaa ccgtgccctg gccgtcctcg
    16561 cccagcagga acacgccatc ctcagggccg acggccgcca cggccaccgc ttcgtctgtc
    16621 ggatcgagcc gtattccgag gcagccacgg acaggcacct gtcgttccat aaaacgaatt
    16681 cccttccctt ctctggaggc taaaaacaga tcgcccgcac cggc
//
LOCUS       DAOMQG010000032        16331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1545_length_16331_cov_12.706930, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000032 DAOMQG010000000
VERSION     DAOMQG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 16331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16331
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_16331_cov_12.706930"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLC06_03465"
     CDS             complement(<1..719)
                     /locus_tag="PLC06_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89533.1"
                     /translation="MNIQKINTGPHQMRFLPVWLLVFAGLLVVTAAVRPLAYAAGDAE
                     PTTMLVSVATNGRAGNQVSSQPAISADGRFVAFTSKASNLVAGDTNRKTDIFVRDMLL
                     GQTERISVATDGSQANDDSTEPAISGNGRFVVFTSTATNLVPTTLNLTDDDWYRGPGI
                     SVFIHDRQAHTTTLVSISSNGVPSDERAWLGDVSYDGRYVIFSSHCEHLVMPDYNGNR
                     RDVFRHDRVTGITRMIDLLPNG"
     gene            1194..2399
                     /locus_tag="PLC06_03470"
     CDS             1194..2399
                     /locus_tag="PLC06_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89534.1"
                     /translation="MTDSFRVELQDVTKRFGDYVAVDDLTLQIRDGEFFSLIGPSGCG
                     KTTTLRMIAGFEQPTDGHIYLGGQAVQGIPAHNRPVNTVFQNYALFPHMTVAQNVAFG
                     LEMQKVTAPEIKRRVAEALELVRLPRMADRKPRQLSGGQQQRVALARALVNRPQVLLL
                     DEPLGALDLKLRRAMQIELKHIQSEVGITFIYVTHDQEEAMTMSDRIGIMSEGVLQQV
                     GSPHEVYEKPVNRFVADFIGETNFLPATVTRLETEEDYPMVALQGGVRVLSANEGHDL
                     RLDQRVTLTIRPERINLYPQGKVDVLKAESGLEAEELERILGAKMPGPVDMKEFLLAE
                     PENVVLNGLLEEAFYIGTDTRFRVTLEGGASLVVRQQNYGSRYDMPFDVGSQVYVQWA
                     AENAQILID"
     gene            2524..3903
                     /locus_tag="PLC06_03475"
     CDS             2524..3903
                     /locus_tag="PLC06_03475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89535.1"
                     /translation="MAVANNTGLSPRSPSPARMTRGADLFFRVAAGLFLGQALLWLTF
                     LLRWATFSVINWRPLAVELALVQWPPVTAYALFGGMAALCLAAGIGLLRETRWAKRLG
                     MAVAVVTLAVGVLYYFLAHEFYGAVTLVGVGGMLLLLLTRRAAWSLAFPSAYWLLIFF
                     LIPLAIVFVVSLGERTRLGTVTFDLSNPATWFNDYGRIFSRINGEFLYLRIFGRSAWL
                     AVLNTVICLVFAYPFAYWIARQPANRRNVLIFLVMIPFWTNFLVRTYAWMLILRDSGL
                     INNFWSITLHEQAVRLAEVSPLFEWLAAATSQKLPLLYNFPAVLLGLFYGYLPFMVLP
                     LYTNLERVNWSLLEAASDLYAGRWQSFRRVLLPLTLPGIIAGSIIVFIPSLGAYVTPD
                     LMGGAKVSLLGNLLQQQFMTVRNWPFGSAISFIMMAVMLAATLVYFRVSNEDDSMAEM
                     TAAQGMLDQ"
     gene            3900..4754
                     /locus_tag="PLC06_03480"
     CDS             3900..4754
                     /locus_tag="PLC06_03480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89536.1"
                     /translation="MTTTNAPLSASQSPPPTARSRAARRDHARAAFNNRVLTWLAIGI
                     FLFLYLPIIILVIYSFSGSRNVGVWAGFSTRWYAELLGDKLLIDAFRVSLWVAVWSTL
                     VATVLGTLAALALERYRFFGRLSFDTALYLPIIIPDIVMALSTLLFFVTFAVPLSRYT
                     ILVTHIAFNIAFVAVVVRARLAEMDASLEEAAADLGANAWTTFRRITLPILMPGVVSG
                     ALLAFTLSLDDFVITFFVAGPGSTTLPVRVYSMIKFGVTPEVNAISTLMLLGSTVLVV
                     ISLWLQRK"
     gene            4900..6051
                     /locus_tag="PLC06_03485"
     CDS             4900..6051
                     /locus_tag="PLC06_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRO89537.1"
                     /translation="MKKYVWIVVLMMLSAAVVACGGGPAGGPGASATEAPAAGAGEGE
                     VQLAPQLSVYNWADYIDEQVLADYTERYGVEIIYDTYASNEDLLAKLQAGASGYDVIF
                     PSDYMVAQMIELGLLAEVDTASLPNFGNIGQEFKDPPYDPGNAHCVPYQWGMTGIAYR
                     NGNPAFAAGPPNSWAWLFDPAVTEKYAAEGINVLNDQRELLGAALFYLGYSPNSTERA
                     ELEAARDLILAAKPQWETFNSEDYYSTLMESDEIVLSHAWSGDAAQAYWNTYDEATGT
                     GNWLFTVPTEGAVRFADNVCIPANSQRQETARHFMNYLMEPEVAAAITNFTFYPSANE
                     AAKEFIDPEILNNPGIYPPPEVAAKLEWLRDVGDAIFIYDEMWTAIKGQ"
     assembly_gap    6056..6155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6156..6448)
                     /locus_tag="PLC06_03490"
     CDS             complement(<6156..6448)
                     /locus_tag="PLC06_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HRO89538.1"
                     /translation="MFKFTVLYYRVDNEMMLEEFFSATHLPLVEALPGLRRLEISRVT
                     GQPFGRSRFFLEVEAYFDSENAMREGLLSQQGLAMMDALRPWSENKLISWFYA"
     gene            complement(6478..8367)
                     /locus_tag="PLC06_03495"
     CDS             complement(6478..8367)
                     /locus_tag="PLC06_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRO89539.1"
                     /translation="MNNHHAAAVKSRLLILVLVSAVALGAMLLLPQAGAAQDPVLPAA
                     TPDALPGLELYAERCANCHGDTGQGDGAMIQQAGQPAPMAFDAAYILNAMPSVMFQQI
                     TNGEAAVGMPPFGPASSNPIEDAGRWNLVAAVYSLATPAENVEAGRAVYAAQCAACHG
                     DTGAGDGPDAATTEPAAPALNDVTYWFNRSNETVFADLAPGAIAAHTYDLPEDDLRRA
                     VDFARTFSYSYADLAALNAPIPAGIIQGTVDNGTTGEVLPKTEVGLRAFTPDFEETLT
                     LTTTTDVDGNFRFDVTDVPPNWIYIAGASFGGLNFSSGANQLSRDTASLDMPITVYDK
                     TSDPAGINIAQLHVVLEFTQDMVQVSQLYVINNVGNTVFVGTSGDPTQGVIEVAVPKE
                     AQNLEFLRSFGTMQSFLPANDFIQTERGWADPLPVRPGEGAQTLLVRYLLPYKSGMTI
                     AHPIFYLTSTSTIILPDAGVSVANTPWVEQEPQAFGQSNSMFRNYVGPSVPAGETITI
                     ELKGRPEVVTDTAGAAVVNRDQTTELLIGGGALLLAAVGGIFLWRFWQKRSAVDDEAW
                     EEETQAETGVSETSETSPAADELIQAIANLDDAFESGTMDEAAYQAQRDQLKQQLTAI
                     WTTDH"
     gene            complement(8388..8990)
                     /locus_tag="PLC06_03500"
     CDS             complement(8388..8990)
                     /locus_tag="PLC06_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO89540.1"
                     /translation="MSSFLSNLTLGSALLGLALVVIVLLYLARPFAMYEDEEVRVNRE
                     EIDGLLLHKADLLRSIRELDDDYDAAKVAPELYAATRPQLMKQAALVMKQLDDHGYVE
                     AGQPVAADIDAQIEAAVRRARTPEQLDEQLEAVIRQARGKATASVATTATTATAAPST
                     NGTAQFCPQCGRRVEKGDRFCPKCGHNHVGEAKSARAARA"
     gene            complement(9002..9589)
                     /locus_tag="PLC06_03505"
     CDS             complement(9002..9589)
                     /locus_tag="PLC06_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRO89541.1"
                     /translation="MNETLQTTPTPAEKAPRRGIRWSMVLLWAAMLAILAVLAWGLIN
                     TTAARPEVGQVAPDFGVEFFDGYEWDTLPVANLSDMRGKPVVLNFWASWCVECRYETD
                     TLEQAWLKYRDDGVVFLGVAYADVEPNSIAYMKEFGVSFPHAPDLGTDISQEYEITGV
                     PETFFIDKDGVIRHVQIGAVDQQMLDTLIPQMLAD"
     gene            complement(9683..10207)
                     /locus_tag="PLC06_03510"
     CDS             complement(9683..10207)
                     /locus_tag="PLC06_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRO89542.1"
                     /translation="MNKDLYVTKIRKKSRLRWLFLALLLVLLPAGVYAQDDPITDDEV
                     NAVAKDVYCPVCESTPLDVCPTAACADWRELIRTKLAEGQSRDEILEYFARQYGDGVL
                     SNPPRRGASLIVLWVLPILGVLAGLLLFGWLLRGMRKPAPEAPAAPVSRPKAATGDPE
                     LDDYIARIEKEVGE"
     gene            complement(10308..11105)
                     /locus_tag="PLC06_03515"
     CDS             complement(10308..11105)
                     /locus_tag="PLC06_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017975236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89543.1"
                     /translation="MHIPDGTMFAGLYGELKLHPWRDFGAAESDAYSLFHSRSVAMGW
                     LAKATEGATGGFWGMNDAGQWGPDPDAQYPYAVWFQVSIPRLIPPTQPLPLQPFLSCV
                     GDVVARMGTLHLDAIQILLPVQSINRSADKSLRDEGLFAPISSAGWFFDCNPHSITRV
                     QVVLDGGENPVIRLAAPDLFQWLQKIKQNVFVCGSFSLTDDTNVGSPTFIDDLWVGPP
                     QHPVTFYGTLIEWSLDALGWLATLLAGASFKFGVTTPMLLTVSRVED"
     gene            complement(11123..11716)
                     /locus_tag="PLC06_03520"
     CDS             complement(11123..11716)
                     /locus_tag="PLC06_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89544.1"
                     /translation="MKLEKQRFWVIVESNAISNVLVCISLLFIISTVLLVPHSVYAQE
                     LELEEDVNDALVHKNHQDVARSNGGLNGLVATGIDPKPSGSICHFRVEGDYVHTSMGD
                     ASGHGWWVNTDCEATQAVVTIRLQQSINGNWVYVGLPGQKTVYSGGGAANRAATRVRC
                     NSSAWTSWRSEVDVDVIGIPDTPGKLYTSVRSLNCRH"
     gene            complement(11958..13949)
                     /locus_tag="PLC06_03525"
     CDS             complement(11958..13949)
                     /locus_tag="PLC06_03525"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRO89545.1"
                     /translation="MIADIGFLTLLLAFLCAVYAVVAAIYGIRKNDDRWVRSARNGLI
                     VVFPLLLLAAGLVITALVTGDFSIAYVSHVSSRGMPTYLKVTALWGGQAGSLLFWNVL
                     LSAFTCAAMLTKWREQKELMPYVIIVSGMTQIFFLLISTFVEDPFTRNVVIPPDGNGL
                     NPLLRHPGMIIHPPMLYLGFVGFTVPYSFAMASLMAKQKGDNWIHTTRRWTLVAWLFL
                     GLGLILGGRWAYDVLGWGGYWGWDPVENASMMPWLAGTAFLHSVMIQEKMRMFKMWNM
                     FLIVLTYCLVILGTFIVRSGVISSVHAFAQSAIGPLFFGFIVIMFVFSAYWITVRRDS
                     LRSENALVSYLSREAAFLYNNFLIMAILAVVFLFTYYPIFSELFTGEKGFVGPPVYER
                     ALAPLFAALVLLMGVAPLTMWYRTSIQRFGRAILWPAVATTAFVVLLFIMGVRLWPAL
                     LGFWLVVFSAALTIMEFVKGTRARMRKGESAWTAFWTLTARNRRRYGGYWIHMGVLIM
                     AFGIVADGIYQTETQIRLQSGESITIGDFEMKFNTVTRFPGADDLLITQADVSLYKNG
                     EFVRTLTPKIELYQRTGQPMTIPSARSTITEDFYVILVNWEPTSADAATFRIFLNPLI
                     NWVWAGGVIFVLGTLIAAWPDREKAPARSRRSVVAVAGD"
     gene            complement(14037..14546)
                     /locus_tag="PLC06_03530"
     CDS             complement(14037..14546)
                     /locus_tag="PLC06_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRO89546.1"
                     /translation="MADVSWQNTDGSLTLEKPRRNRLMFVVGGIVLISAVVFLVVNAM
                     SGSTQLYKTVNEFYADQGRLVGRDLRVSGWVIGDTIQYTQIDAHNSRLEFDITDDLTN
                     PAGQRLHVVVLNEPRPDLLQHEAQALVEGHGDANGIFTSDPGGLMLKCPTRYEEMDPA
                     GHPDSVPTS"
     gene            complement(14571..14876)
                     /locus_tag="PLC06_03535"
     CDS             complement(14571..14876)
                     /locus_tag="PLC06_03535"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRO89547.1"
                     /translation="MDTDNIHPLPEADRTPGESREISWVSVATSPGITVAEIIAGRLQ
                     SEGIPARAWQEGAGEALGLIVGLLGTGHVVVPAEYAAEARAILEADEADMIEEEEEE"
     gene            complement(15393..15542)
                     /locus_tag="PLC06_03540"
     CDS             complement(15393..15542)
                     /locus_tag="PLC06_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89548.1"
                     /translation="MNEFQGTTVIVVLFALRCVMPFVLMLALAYGMKRLVRHWDSEET
                     EPLQT"
     gene            complement(15780..>16331)
                     /locus_tag="PLC06_03545"
     CDS             complement(15780..>16331)
                     /locus_tag="PLC06_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89549.1"
                     /translation="NGEEAIRRTAELRPDVVLMDIEMPGMNGIEAARRIKAQSPGTSV
                     LALTMYEDDQYFFEMLRAGASGYVPKRAAPDELASAIRAVSRGEVFIHPSLAGRLVQD
                     YLQRRDVEVQEQPTGDLTPREQEVLTLIAQGLSNNEIADQLVISAKTVDRHRENIMRK
                     LNLHNRVDLVKYALRKGLIDLEE"
BASE COUNT         3222 a         4831 c         4897 g         3281 t
ORIGIN      
        1 ccattgggca ggagatcgat catgcgggtg atgccggtaa cgcggtcatg acgaaaaacg
       61 tcgcgacgat tgccgttgta gtccggcatc accagatgct cgcaatgaga cgagaagatc
      121 acgtagcgac catcgtatga aacatcaccc aaccaggcgc gctcatcaga cgggacgcca
      181 tttgagctaa tggagacgag tgttgtcgtg tgggcctgac ggtcgtggat aaatacgctg
      241 atacctgggc cgcgatacca gtcgtcatcc gtaagattca gcgttgttgg cacaaggttg
      301 gttgcggttg acgtgaaaac aacgaagcgc ccgttacccg agatggccgg ttccgtcgag
      361 tcatcattgg cctgactccc gtctgtggct acggagatac gctcggtctg gccaagcagc
      421 atatcgcgaa caaagatgtc ggtcttgcgg ttggtatcac cagctaccag gttggaagct
      481 ttggacgtga acgcgacgaa gcgaccatcg gccgaaatgg ccggttggga tgaaacttga
      541 ttcccagcgc gcccgttggt ggctacggaa accagcatcg tggtgggttc agcgtcgccg
      601 gctgcatatg ccagtggccg cacagcggca gttacgacca gcaaaccggc aaataccagc
      661 agccaaacag gtaaaaatcg catttggtgt gggcctgtgt tgatcttttg gatgttcatg
      721 gtatgccttg tctgaaagcc tgggactgat gttactcaac gacgggagta cagtccggta
      781 tgggcactgg ggacataaac gccaatatcc ctacattcag gttaacatca aggagaccat
      841 cgagggtagt gacattcgtc atatatcttt cgtgacgatc gtctaatgtg gacagttttg
      901 gttcatggtt tcattgtcaa atcagcgcaa tcgtagtatg gtaatagggt cattacaaga
      961 gcgcgagcca cctatggacg aatcagttca ttgaagagcg ccgggtgggt ggctgcgaat
     1021 atcgacagtg caagttctgg cactggttgc cgagaggcga tcggggctgg gctttttttt
     1081 cgcactgagg gcttttgagt gaaagacctg actgttgtcg gaacatctgt caggtctaat
     1141 aatcaaaggc attccggccg tgtcatagcg gcgagtttta ttgggagatt gctatgacag
     1201 attcgttccg cgtggaactt caagacgtca ccaaacgctt cggcgactat gtcgccgtcg
     1261 acgacctcac cctgcaaata cgcgacggtg agttcttctc gcttatcggc cccagcggct
     1321 gcggcaaaac gaccaccctg cgcatgatcg ccgggttcga gcagccgaca gatggccaca
     1381 tctacctcgg cggccaggcc gttcagggta tccccgcgca taaccgcccg gtcaacactg
     1441 tcttccagaa ctacgccctt ttcccgcata tgaccgtggc ccagaatgtg gcttttggtc
     1501 tggagatgca aaaagtgacc gcgccggaga tcaagcggcg cgtggccgag gcgctggaac
     1561 tggtgcgctt gccgcggatg gccgaccgca agccgcggca gctctccggc ggccaacagc
     1621 agcgcgtggc gctggcgcgg gcgctggtga atcggccgca agtgcttctg ctcgacgaac
     1681 cgctgggcgc gctggacctg aagctgcgcc gggcgatgca gatcgaactc aagcatatcc
     1741 agagcgaggt cggcatcacc ttcatctacg tcacccacga ccaggaagaa gcgatgacga
     1801 tgtccgaccg gataggcatc atgagcgagg gcgtcctgca gcaggtcggc tcgccccacg
     1861 aagtctacga gaagccggtc aaccgttttg tggccgattt catcggcgag accaattttc
     1921 tgccggccac tgtgacgcgg ctggagacgg aagaggatta cccgatggtg gccctgcagg
     1981 gtggtgtgcg cgttctgtcc gccaacgagg ggcacgatct gcggctcgac cagcgggtga
     2041 cattgaccat ccggccggag cgcatcaatc tctatccgca gggcaaggtc gatgtgctga
     2101 aggccgagag cggcctggag gctgaggagc tggagcgtat tctgggggcc aagatgcccg
     2161 gcccggtgga tatgaaggag tttctgctgg ccgagccgga gaacgtggtg ctgaacggcc
     2221 tgctggaaga agcattctac atcggcaccg acacgcgctt ccgggtcacg ctggagggcg
     2281 gcgcgtcgct cgtcgtccgt caacagaact acggttcgcg ctatgacatg ccgttcgacg
     2341 tgggcagcca ggtctatgtg cagtgggcgg ccgagaatgc gcagatattg attgattagc
     2401 agtaggaacc tttagaaacc gggtttcccg gaagaagccc ggtttccagg agcgccgtat
     2461 gagtaccaac tatcgcaaac attacgccgg cggcagccga ttccacaaca gcaggaggag
     2521 ccgatggctg tcgcgaataa caccgggctg agcccgcgat ctccatctcc ggcccgcatg
     2581 acgcgtggcg cggacctctt cttccgcgtc gccgccggtc ttttcctcgg ccaggctctc
     2641 ctctggctca cctttctcct tcgttgggca accttctctg ttatcaactg gcggccgctg
     2701 gccgtcgagc tggcgctggt tcaatggccc ccggtgaccg cctacgcgct tttcggcggg
     2761 atggcggctc tatgcctggc ggcggggatt ggcttgctgc gcgagacgcg ttgggcgaag
     2821 cgcctgggga tggccgtcgc ggtagtcacc ctggcggtgg gcgttctcta ctacttcctg
     2881 gcgcatgagt tctatggcgc ggtgacactc gtcggggtgg gcgggatgct actgctgctt
     2941 ctgacgcgcc gcgccgcctg gtcgctggcc tttccgtcgg cctactggct gctcatcttc
     3001 ttcctcatcc cgctggccat cgtcttcgtc gtcagcctcg gcgagcgcac gcggctgggt
     3061 acggtgacgt tcgacctgtc caatccggcc acctggttca acgactacgg ccgtatcttc
     3121 agccgcatca atggggagtt cctctatctg cgcatcttcg gccgttcggc gtggctggcc
     3181 gtgctcaaca cggtcatctg cctcgtcttc gcctatccgt ttgcctactg gatcgcccgc
     3241 cagccggcca accggcgcaa tgtgctcatc tttctggtga tgatcccgtt ctggaccaac
     3301 tttctggtgc gcacctacgc ctggatgctc atcctgcgcg attcggggct gatcaataac
     3361 ttctggagca tcacgcttca tgagcaagcg gtacggctgg ccgaggtgtc gccgctgttc
     3421 gaatggctgg ccgcggccac gtcgcaaaaa ttgccgctac tctataattt tccggccgtg
     3481 ctcctcggcc tgttctacgg ctacctgccg ttcatggtgc tgccgctcta caccaatctg
     3541 gaacgggtca actggtcgct gctggaggcg gcgtcggacc tctacgccgg gcgctggcag
     3601 agcttccggc gggtgctgct gccgctcacg ctgccgggca tcatcgccgg gtcgatcatc
     3661 gtcttcatcc cgtcactggg cgcctatgtg acgcccgatc tgatgggcgg ggcgaaggtc
     3721 tccctgttgg gcaacctgtt gcagcagcag ttcatgactg ttcgtaattg gccgttcgga
     3781 tcggccatca gcttcatcat gatggccgtc atgctggcgg cgacgctggt ttatttccgc
     3841 gtctccaacg aggatgactc gatggcggag atgacggccg ctcaggggat gctggatcaa
     3901 tgaccacgac gaacgcgcct ctctccgcat cccaatcgcc gccacccacc gcccggtcgc
     3961 gcgccgcgcg ccgcgaccac gcacgcgcgg ccttcaacaa tcgtgtcctg acctggctgg
     4021 cgatcggcat tttcctgttt ctctatctgc cgatcatcat cctggtcatc tactccttca
     4081 gcggctcgcg caacgtcggc gtgtgggccg gcttcagcac gcgctggtac gctgagctat
     4141 tgggcgacaa gctgctgatt gatgcttttc gcgtcagcct gtgggtggcc gtgtggtcga
     4201 cgctggtggc cactgtgctg ggcacactgg cggcactggc gctggagcgt taccgtttct
     4261 tcggccgcct gtcgttcgac acggcgctct acctgccgat catcatcccc gatatcgtca
     4321 tggccctgtc gacgctgctg ttcttcgtca ccttcgccgt gccgctcagc cgctacacca
     4381 tcctggtgac ccacatcgcc ttcaacatcg ccttcgtggc cgtggtagtg cgggcgcgac
     4441 tggcggagat ggacgcttcg ctggaagaag ccgcggccga cctgggggcc aacgcctgga
     4501 caaccttccg gcgcataacc ttgcccattc tgatgccggg cgtcgtttcc ggcgcattgc
     4561 tggcctttac gctgtcgctc gatgattttg tcatcacttt cttcgtcgcg gggccgggtt
     4621 ccaccacgct gccggtgcgc gtctattcga tgatcaagtt cggcgtcacg cccgaggtta
     4681 acgccatctc gacgctgatg ctgctggggt cgacggtgct ggtggtgatt tcgttgtggc
     4741 tgcagaggaa gtaaccggtg agggagtgtt cagtgaggca gtcgatcagt tattcagtag
     4801 gtcagtattc agtaggtcag tagattgatg gtctgtcgtg gggggggcgg agtgattctg
     4861 tgtccacgac ggataatttg agtgaggaaa gagaagaaaa tgaaaaaata tgtatggatc
     4921 gtggtattaa tgatgttgtc ggcggccgtt gttgcctgtg ggggcggccc ggccggtggg
     4981 cctggcgctt cggcgaccga ggcccccgcg gccggagccg gcgagggtga agtccagctt
     5041 gccccacagc tcagcgtcta taactgggcc gactatatcg acgagcaggt gttggccgac
     5101 tataccgagc gctatggggt cgagatcatc tacgacacct atgcgtccaa cgaagacctg
     5161 ctggccaagt tgcaagccgg agccagcggc tacgacgtca tcttcccgtc ggattacatg
     5221 gtggcccaga tgatcgagct ggggctgctg gccgaggtgg atacggcgtc gctgccgaac
     5281 tttggcaaca tcggccagga gttcaaggat ccgccctatg atcccggcaa cgcccactgt
     5341 gtaccctacc aatggggcat gaccggcatc gcctatcgca acggcaaccc ggccttcgcg
     5401 gcggggccgc ccaacagttg ggcctggctg ttcgatccgg cggtgacgga gaagtacgcg
     5461 gccgagggga tcaacgtgct caacgaccag cgcgagctgc tgggcgcggc gctgttctac
     5521 ctgggttatt cgcccaactc gacggagcgg gccgagctgg aagcggcgcg agacctgatc
     5581 ttggccgcca agccgcagtg ggagaccttc aacagcgagg actattacag cacgctgatg
     5641 gagagtgacg agatagtcct gtcccacgcc tggagtggcg acgcggcgca ggcctactgg
     5701 aatacctacg acgaggccac gggcacgggc aactggctgt tcactgtgcc gacggaaggc
     5761 gcggtgcgct ttgccgacaa cgtttgcatt ccagccaaca gccagcggca ggagacagcg
     5821 cgccacttca tgaactacct catggagccg gaagtggccg cggccatcac caacttcacc
     5881 ttctacccca gcgccaacga agcggccaag gagttcatcg accccgaaat cctgaataat
     5941 cccggcatct atcccccgcc ggaagtggcc gccaagctgg agtggctgcg cgacgtgggc
     6001 gacgcgatct tcatctatga tgagatgtgg acggcgatta aggggcagta ggtcannnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcggc gtagaaccag ctgatgagct
     6181 tgttctccga ccacggccgc agcgcgtcca tcatggctaa cccctgttgc gacagcaatc
     6241 cctcgcgcat cgcgttctca ctgtcgaaat aggcctcaac ctccaggaag aagcgcgagc
     6301 ggccgaacgg ttgcccggtg acgcggctga tctccaggcg gcgcagcccc ggcaaggcct
     6361 ccaccagcgg cagatgggtg gcgctgaaga actcttcgag catcatctca ttgtcgacgc
     6421 ggtagtagag gacggtgaat ttgaacatgg gggtcagtgg atcagtaggt cagtgggtca
     6481 gtgatcagtg gtccagattg cggtcagctg ttgtttcagt tggtcgcgct gggcctggta
     6541 ggcagcttca tccattgtgc ccgattcaaa ggcgtcgtcg aggttggcga tggcttggat
     6601 gagttcatcg gcggccgggc ttgtttcaga tgtttcagac acgcccgttt cggcctgggt
     6661 ttcctcttcc cacgcttcgt catccacggc cgaccgcttt tgccagaagc gccacaggaa
     6721 aatgccgccg acggctgcca gcagcagcgc cccgccgccg atcagcagct cggtcgtctg
     6781 atcccgattg acgacggccg cgccggccgt gtccgtcacc acttccggcc ggcccttcag
     6841 ttcaatggtg atcgtctcgc ccgcgggcac actcggcccc acgtagttgc ggaacatgct
     6901 attactctga ccaaaagctt gcggctcctg ctccacccat ggcgtattgg ccaccgacac
     6961 cccggcgtcg ggcaggatga tcgtgctggt cgatgtcagg tagaagatgg ggtgggcgat
     7021 ggtcatgccg cttttgtagg gcagcagata gcgcaccagc agcgtctgcg caccctcgcc
     7081 cggccgcacc ggcaaggggt cggcccagcc gcgttcggtc tggatgaagt cgttggccgg
     7141 caggaaggac tgcatcgtgc cgaacgagcg caggaactcc aggttttgcg cctctttggg
     7201 cacggccacc tcgatcacgc cctgggtcgg gtcgccgctt gtgccgacaa agaccgtgtt
     7261 cccgacgtta ttgatgacgt atagctggct gacctgcacc atgtcctgtg tgaactccag
     7321 aaccacgtgt agctgggcga tattgatgcc ggccggatcg ctggtcttgt cgtagacggt
     7381 gatgggcatg tcgagcgacg cggtgtcgcg gctcaactgg ttcgcgccgc tgctgaagtt
     7441 cagcccgcca aacgacgctc cggcgatata gatccagttc ggcggcacgt cggtcacatc
     7501 gaagcgaaaa ttgccgtcga cgtcggtcgt cgtcgtcagc gtcagtgtct cctcgaaatc
     7561 aggtgtgaaa gcgcgcaggc ccacctcggt tttcggcaat acctcgccgg tggtgccgtt
     7621 atcgaccgtg ccctggatga tcccggccgg gatgggcgcg ttcaaggcag ccaggtcggc
     7681 gtagctatag ctgaatgtgc gcgcgaaatc gaccgcccgc cgcagatcgt cttcgggcaa
     7741 gtcataggta tgggcggcaa tcgcgcccgg agccaggtcg gcaaataccg tctcgttgct
     7801 gcgattgaac cagtaggtga cgtcattgag ggccggggcg gctggttcgg tggtcgccgc
     7861 gtcggggccg tcgcccgcgc ccgtgtcgcc gtggcaggcg gcacattggg cggcatatac
     7921 cgcgcgcccg gcttccacgt tctccgccgg tgttgccagg ctgtagacgg ccgccaccag
     7981 attccagcgc ccggcatcct caatggggtt gctgctggcc gggccgaacg ggggcatgcc
     8041 cacggccgcc tcgccgttgg tgatctgctg gaacatgacc gacggcatgg cattgaggat
     8101 ataggcggcg tcgaaagcca tgggagcggg ctgaccggcc tgctggatca tcgcgccgtc
     8161 accctggccg gtgtcgccgt ggcagttggc gcagcgttcg gcgtagagtt caagacccgg
     8221 caatgcgtcg ggcgtggccg cgggcaacac ggggtcctgg gccgcgccgg cttgcggcag
     8281 cagcagcatg gcccccaggg caaccgcgga aacgaggacg agtatcagca ggcggctctt
     8341 gacagccgcg gcgtggtggt tgttcatagg atcgatttgg tagtggctca ggcgcgcgcg
     8401 gcccgcgcgg atttggcttc cccgacgtgg ttgtggccgc atttggggca gaagcgatcg
     8461 cctttttcga cccggcggcc gcattgcggg caaaactgcg ccgttccgtt ggtcgacggc
     8521 gcggccgttg cggttgttgc ggtcgtcgcg accgaagcgg tcgctttccc gcgcgcctgg
     8581 cggatgaccg cttcgagttg ctcgtccagt tgctccgggg tgcgcgcgcg ccgcacggcg
     8641 gcctcgatct gcgcgtcgat gtcggccgcg acaggttgcc ccgcttccac ataaccatga
     8701 tcatcaagct gcttcatgac cagggccgcc tgcttcatca actgcggtcg ggtggccgcg
     8761 tatagctccg gcgcgacctt ggccgcgtcg taatcatcat ccagctcgcg gatgctgcgc
     8821 aggagatccg ccttgtgcaa aagcagcccg tcgatctcct cgcgattgac gcgcacttcc
     8881 tcgtcctcgt acatggcgaa cggccgcgcc agatagagca ggacgatgac gaccagcgcc
     8941 aggcccagca aggccgaacc caacgttaaa ttcgatagaa aagaactcac ggcagccccc
     9001 tctaatcggc cagcatttgc gggatgagcg tatccagcat ctgttgatca accgcgccaa
     9061 tctggacgtg gcgaatgacg ccgtccttgt cgatgaagaa agtctccggc acgccggtga
     9121 tctcatactc ctgcgagatg tcggtgccca ggtcgggcgc gtgggggaag gagacgccga
     9181 attccttcat gtaggcgatg gagttcggct ccacatccgc ataggccacg ccgaggaaga
     9241 cgacgccgtc atcgcggtac ttcagccagg cctgttccag cgtgtcggtc tcatagcgac
     9301 actcgacgca ccacgaggcc cagaaattca gcaccaccgg cttgccgcgc atatccgaca
     9361 ggttagccac cgggagagtg tcccattcgt agccgtcgaa gaactcgacg ccgaagtcgg
     9421 gggccacctg ccccacctcg gggcgcgcgg cggtggtatt gatcaacccc caggccagca
     9481 ccgccaggat cgccagcatg gctgcccaga gcagcaccat cgaccagcga atgcccctgc
     9541 gcggcgcttt ctcggccggg gtgggtgtgg tttgcagtgt ttcgttcata atggttttcc
     9601 atcaagacct gacaggtggg cggcacaagc tcatgttcca gtgcaaggat tttgtccgcc
     9661 cacctgtcag gtctgtcata ttctactcgc cgacctcttt ctcaatgcga gcgatatagt
     9721 catcaagttc cgggtcgccg gtggccgcct tcggccgcga gaccggcgcg gcgggcgctt
     9781 ccggcgcggg cttgcgcatg ccccgcaaca gccagccaaa gagcaacaga ccggccaaca
     9841 cgcccagaat gggcaacacc cacaggacga tcagactggc cccgcggcgc ggcgggttcg
     9901 acagcacgcc gtcgccgtat tgccgggcga aatactccag aatttcgtcg cgcgattggc
     9961 cctcggccag cttggttcga atcagctcgc gccagtcggc gcaggccgcc gtgggacaga
    10021 cgtccagcgg cgtgctttcg cacaccggac agtagacgtc cttggccacg gcgttcacct
    10081 cgtcgtcggt gatggggtcg tcctgagcgt aaacgcccgc aggcaacagg accagcaaca
    10141 gggccaggaa taaccagcgc aagcgcgatt tcttgcggat ttttgtcaca tataagtcct
    10201 tattcaaaag ggtttgcact tttaagggca cgtttacata aacatcagcc ataattttgt
    10261 cgataaacct ttcgaatatc caagcaatat tcgcctgatg cgcgaaccta gtcctcaact
    10321 cggctaactg tgagcaacat gggagtggtc actccaaatt taaaacttgc accagcaagg
    10381 agagtagcca accaccccag ggcatccaga gaccactcga tcagagtacc gtaaaacgta
    10441 accggatgtt gtggggggcc aacccataga tcatcaataa aggtggggct ccccacgtta
    10501 gtatcatctg ttaatgagaa ggagccacac acaaaaacat tttgcttaat cttttgcaac
    10561 cattgaaaca gatcaggtgc ggccaagcgg ataactggat tctcaccgcc atccaatacc
    10621 acttgcacgc gagttataga gtgcggattg caatcaaaaa accagccagc actactgatc
    10681 ggagcaaaca gaccttcatc gcgtaatgat ttatctgccg atctattaat actctgtaca
    10741 ggcagcaaga tctggatagc atcaagatgc aaagttccca tacgtgctac gacatcaccc
    10801 acacatgaga ggaacggttg cagaggcaac ggctgggtgg gaggaatcaa tcggggtatt
    10861 gacacctgaa accacaccgc atatggatac tgagcatcag gatcagggcc ccactgccca
    10921 gcatcattca taccccaaaa gccacctgtt gcaccctccg tagccttggc gagccatccc
    10981 atggcaacag acctcgaatg aaacaaacta tacgcatctg attcggccgc accgaagtct
    11041 cgccacgggt gcagtttcaa ttcaccatac aagcctgcaa acatggtgcc atcaggtata
    11101 tgcattaagt caccctttaa tattaatgcc tgcaattaag acttctgacc gacgtataga
    11161 gtttacctgg agtatcagga ataccgatga catctacatc aacttcgcta cgccacgacg
    11221 tccaagcact gctattacaa cggactcgag tagcggcacg attggcagca cccccaccgg
    11281 aatacaccgt tttttgaccg ggtaagccta catataccca attaccgtta atactctgtt
    11341 gcaacctaat tgtcacaact gcctgcgtag cctcacagtc agtgtttacc caccatccat
    11401 gtccagacgc atctcccatg gatgtatgga catagtctcc ttccacacga aaatgacata
    11461 tcgaaccact gggtttggga tcaatgccag tagctaccaa gccgttcagg ccaccattgc
    11521 ttctggctac gtcttgatga tttttgtgaa caagggcatc gttgacgtct tcctctaact
    11581 ccaactcttg tgcatagaca ctgtgcggta ccaagagaac agtacttata ataaacagca
    11641 aactaataca gaccagcaca tttgagatgg cattgctttc tacgattacc caaaaccttt
    11701 gcttttcgag tttcatgata gaccttcatt tccctttaag tgttgcatct taaaagacca
    11761 agaatagcat cctggaacgc tgtattgaac atttaaaatt ttaatcacgt attgctctcc
    11821 agcgtgttca aatctgctgc ttcttgggtg atgtcggttc caaccagccc ccagcagagg
    11881 ctggcgacca actatagccg gttttgtatg aaagtagcgg gatggattcc cgcgtgaata
    11941 gtaatgctac gaacctctta atctcctgca acggcaacca ccgaccggcg cgagcgggcc
    12001 ggggcctttt cgcggtccgg ccaggcggcg atgagcgtgc cgaggacgaa gatgacgccg
    12061 cccgcccaaa cccagttgat gagggggttc aggaagatgc ggaatgtggc cgcgtcggca
    12121 ctcgtcggct cccagttgac caggatgacg tagaagtcct cggtgatggt cgatcgggcc
    12181 gaggggatgg tcatcggctg gccggtgcgc tggtatagct cgatcttggg cgtcagcgtg
    12241 cgcacgaatt cgccattctt gtagaggctc acgtcggcct gcgtaataag caaatcatcg
    12301 gctccgggga agcgggtgac ggtgttgaac ttcatctcga agtcaccgat cgtgatgctt
    12361 tcgccggatt gcaggcggat ctgggtctcc gtctgataga tgccgtcggc gacgatgccg
    12421 aaggccatga tcagcacgcc catgtggatc cagtagccgc catagcgtcg ccggttgcgg
    12481 gcggtcaacg tccagaacgc cgtccaggcc gattcgccct tgcgcatgcg cgcccgcgtg
    12541 cccttgacga actccatgat cgtcagcgcg gccgagaaca cgaccagcca gaagccgagc
    12601 agcgccggcc acaggcgaac gcccatgatg aacagcaaga cgacgaatgc cgtcgtggcc
    12661 accgccggcc acagaatggc gcggccgaat cgctggatgc tggtgcggta ccacatcgtc
    12721 aatggggcca cgcccatcag cagcaccagc gcggcgaaca gcggcgccag tgcccgctca
    12781 tagacaggcg gcccgacgaa acccttctcc ccggtaaaca gttcggagaa gatgggatag
    12841 taggtgaaca ggaagaccac ggccaggatg gccatgatca ggaagttgtt atagagaaag
    12901 gccgcctcgc gcgacagata ggaaaccagc gcgttttccg agcgcaggct gtcgcgccgc
    12961 accgtgatcc agtaggccga gaagacgaac atgatcacga tgaagccgaa gaagagcggg
    13021 ccgatggccg actgggcaaa ggcgtggacg gacgagatga cgccgctgcg cacgatgaac
    13081 gtgcccagta tgaccagaca gtaggtcagc acgatgagga acatgttcca catcttgaac
    13141 atgcgcatct tttcctggat catgaccgaa tgcaggaagg ccgtgcccgc cagccagggc
    13201 atcatcgagg cgttctccac cgggtcccag ccccagtagc cgccccagcc cagcacgtcg
    13261 taggcccagc ggccgccgag gatcagcccc agccccagga acagccacgc caccagcgtc
    13321 cagcggcggg tggtgtggat ccagttgtcc cccttctgct tggccatcag cgaggccatg
    13381 gcgaaggaat aggggacggt gaagccgacg aagcccaggt agagcatggg cggatggatg
    13441 atcatccccg gatggcgcag cagcgggttg aggccgttgc cgtcgggggg aatgactacg
    13501 ttgcgcgtga aggggtcttc gacgaaggtc gagatgagca ggaagaagat ctgggtcatg
    13561 ccgctgacga tgatgacgta gggcatcagc tctttctgct cgcgccattt ggtcaacatt
    13621 gccgcgcagg tgaaggccga cagcagcacg ttccagaaca gcagcgagcc ggcctggccg
    13681 ccccagagcg cggtcacttt cagataggtc ggcatgccgc ggctgctgac gtgggagacg
    13741 taggcgatgg agaagtcgcc ggtcaccagc gccgtgatga ccagccccgc ggccagcaac
    13801 aggagcggga agacgacgat caacccattg cgcgcgctgc gcacccaacg gtcgtcgttt
    13861 ttccgaatac catagatcgc cgccactacg gcatagacgg cgcacaggaa agcgagcaac
    13921 aatgtgagaa agccgatatc agcgatcatg cttctttacc ctgaaaacaa aacgggccgg
    13981 cgacgcgccg ccgcgtcatt gacccgtcat gaaatgcctg aaatataaac cgccggttat
    14041 gaggtcggaa cgctgtcggg atgcccggcc ggatccatct cttcatagcg ggtcgggcac
    14101 ttgagcatta gcccgcccgg gtcggatgtg aatatgccgt tggcgtcgcc gtggccttcg
    14161 accagcgcct gggcctcgtg ctgcagcagg tcgggtcgcg gctcgttgag caccacaacg
    14221 tgcaatcgtt ggccggccgg attggtcagg tcgtcggtga tgtcgaactc cagccgggag
    14281 ttgtgggcgt cgatctgggt gtactggatg gtgtcaccga tgacccaccc ggaaacgcgc
    14341 agatcgcggc cgacgagacg cccctggtcg gcgtagaact cgttgacggt cttgtacaat
    14401 tgtgtgctgc cactcatggc attaacgacg agaaatacga cggcggaaat caacacgata
    14461 ccgccgacga cgaacatcag acgattccgg cgcggtttct ccagcgtcag ggagccatcg
    14521 gtattctgcc agcttacatc tgccatcttt cttgctcctt cggtgaaagg ctactcttct
    14581 tcctcttctt cgatcatgtc ggcctcgtcg gcctccagga tggctcgcgc ctccgcggca
    14641 tactccgcgg gcacgacgac gtgaccggta cccagcaaac cgacgatgag gcccaacgcc
    14701 tcgcccgcgc cttcctgcca ggctcgcgcc gggatccctt cggactgcaa gcgcccggcg
    14761 atgatctcgg ccacggtgat gcccggcgac gtggcgacgc tgacccagga aatttcccgg
    14821 ctctcgcccg gtgtgcgatc ggcttcaggg agtggatgaa tgttgtctgt atccataatg
    14881 attcgttgtt ttggcctgcc tgtttgttat tcaaaaaatt ataccgcaag ctgcctcaat
    14941 tagatagcaa tctaaggaaa caccgaactc atggttcggc acaacattca taagactcac
    15001 ttattataga caagtgccga ctgttttcta gcgccaattg tcatctgtcg ggcatgacgg
    15061 atgtcatgga taggcaaggt taccgcgcaa cccgggcgga ttgcgtcttc gggggcaagt
    15121 tatcagctct tgctccaggg caaagataaa agtttggtgg agtgattatc tagtcaccgg
    15181 ccgcgggaat agctgttcca gtatacaacg cacagccggc gcactgggcc ggaatccggc
    15241 cgtcggcgcg caatcgtgct acccagcagg ccaatgatgg actgatatag accgggcagg
    15301 cgctgcgcgt ctttgttcag cagccaacag gggagcgcag acttgatggg ttcggccgcc
    15361 gagtgggcgg tcatgagcag caggatagac ggctaagtct gcagtggttc tgtctcttcg
    15421 ctgtcccaat gcctgaccag ccgcttcatg ccataggcga gggccagcat cagcacaaac
    15481 ggcatcacgc agcgtagcgc gaacaaaacc acaatgaccg tcgtcccctg gaattcgttc
    15541 atctcgtctc tgacctgggc tcatatcccc atcggccgag ccaatcggct ttcatatatc
    15601 tcctgactac aggatacctg ctaacgagcc tgttcgtcta ttgggcaagc gacacatttt
    15661 ggtgatgggg gaaacaaccc acgaagaagg gcgactcaag cctcgactac tgacccactg
    15721 accagtagat cagtatccgg tagatcagta ttcagttact gacctactga cctactgacc
    15781 tactcctcca aatcaatcaa ccccttccgc aaggcatatt tgaccagatc gacccggttg
    15841 tggaggttga gcttgcgcat gatgttctcg cggtgtcggt caaccgtttt ggcgctgatg
    15901 accagttggt cggcaatttc gttgttgctc aggccctggg cgatgagcgt cagcacttcc
    15961 tgctcgcggg gggtcaggtc gccggtgggc tgctcctgca cttccacatc gcgccgctgc
    16021 aggtagtcct gcaccagccg cccggccagc gacggatgga tgaacacctc gccgcggctg
    16081 acggcccgaa tggccgacgc caattcgtcc ggcgcggcgc gcttcggcac gtagcccgac
    16141 gccccggcgc gcaacatctc gaagaagtac tggtcgtcct catacatggt cagggccaga
    16201 accgacgttc ccggtgattg ggccttgatg cgtcgcgccg cctcgatccc attcatgccc
    16261 ggcatttcga tgtccatcag caccacgtcg ggccgcaact cggccgtgcg ccggatggcc
    16321 tcctcgccgt t
//
LOCUS       DAOMQG010000033        16327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1548_length_16327_cov_6.422874, whole genome shotgun sequence.
ACCESSION   DAOMQG010000033 DAOMQG010000000
VERSION     DAOMQG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 16327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16327
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_16327_cov_6.422874"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLC06_03550"
     CDS             <1..403
                     /locus_tag="PLC06_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89550.1"
                     /translation="PTNTPGPDPTATPTSTPPAAGMHIGDLDGSSVYTNNNNRWKASV
                     TFTVHNGSHGVVSGARVTGKWSNGASGNASCTTNAAGQCSINKPKLLTNVSSVTFTVT
                     KVTRSGQTYSAASNHDPDGDSNGTYIVVQKP"
     gene            697..1593
                     /locus_tag="PLC06_03555"
     CDS             697..1593
                     /locus_tag="PLC06_03555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRO89551.1"
                     /translation="MAQKIIRCTDRHFERMLEFVGAHNPASEHHIGYFGVTPADIRQS
                     VLMLNLRYNRGFRLALEGSRLVGVMGMDFDKEIGRAWLYGPIVVADGSAADWTATADA
                     LYAGVTPVLPVGIHEHEMFVDTENAHCRAFAARHDFHNQGEMEIYFITPDRLAALPEA
                     TAEEWDGRFADRFIALHDQLFPRSNFTLNSMLSERDKGATLLLLTDGDDLAGYFFGRA
                     DAVSGEAFVDFVGVDPARHGQGLGRRLMLAGLARQRVTPNLHQVNLTVKPDNLPAMRL
                     YESLGFVRERGMVAFRKKVGQD"
     gene            1633..2859
                     /locus_tag="PLC06_03560"
     CDS             1633..2859
                     /locus_tag="PLC06_03560"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89552.1"
                     /translation="MIPKKILILTADAGFGHRSTANALCRAFELRYGEAAQVIIVNPL
                     DDVTALFFLRSAENDYDKLAREWPQFYKLGYRMIDLSLTTNVTEMAYVMMMYDPISRL
                     MKQHEPDVVVITFPTYQYPVAAYRRITGARVPIVTVVTDLVSLQKMWFSQSVDMCLVP
                     TREAAAAATERNMDPESIHITGLPVNPALAAVPASKTEARRALNWPEDKFTVLAVGSK
                     RVEALETFANVLNHSGHDLHFVGVAGGNDQLYTALRDAEWHIPATIYDYITNMPDLMH
                     AADCIVSKAGGLIVTESLACGLPMLITQVIPGQETGNAKYMVDGGAGDLTMEPLQFLE
                     TMTHWLADDRRLYKERAANARRLGRPGAAFAAADLIWDLTKAGAARHRSRLPVSREKL
                     RELLRQFSGTEKPVPS"
     gene            2954..3562
                     /locus_tag="PLC06_03565"
     CDS             2954..3562
                     /locus_tag="PLC06_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89553.1"
                     /translation="MRKLVTLWLVTLLLLAACSAGGANNEGQATGTHDDVAAITLITP
                     ESGGGYPAPANPYPVSPVEALLPTDYPGMAISTMPPTDLTPDAGANSAATSPPTDRPL
                     ATPYPVPDRVLATLVRELSALADVPIDEIDVAAAEPVVWPNAGLGCPAEGMAYAEVQV
                     EGMLIVLAAGGRDYTFHTDGAQRYVLCRDGRPISEGNIKDRG"
     gene            complement(3572..4252)
                     /gene="rpe"
                     /locus_tag="PLC06_03570"
     CDS             complement(3572..4252)
                     /gene="rpe"
                     /locus_tag="PLC06_03570"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRO89554.1"
                     /translation="MRPIRLAPSILSADFSRLGEQVEEALAAGADYIHVDVMDGHFVP
                     NITIGPPIVRAVAPIARRYGATVDVHLMIEKPERYLADFARAGADILTVHVETCPHLH
                     RTVEAIREVGARPGVTLNPATPLVALEEILPYVDLVLIMSVNPGFGGQSYIPTSTDKI
                     RRLRAILDFIGSTAELEVDGGIKPDNAAEIVAAGASALVAGSAIFGGSQTVAGNIAAF
                     RAALSNGS"
     gene            complement(4249..5244)
                     /gene="glpX"
                     /locus_tag="PLC06_03575"
     CDS             complement(4249..5244)
                     /gene="glpX"
                     /locus_tag="PLC06_03575"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRO89555.1"
                     /translation="MSMSEAPDRNLALELVRATEAAALAAARWVGRGQKEEGDGAAVD
                     ALRLLLGSVRMDGIVIIGEGEKDEAPMLYNGEIVGNGRGPQVDVAVDPVEGTNLLAKG
                     LPNSISVVAIAARGTLFNPGPAFYMDKLVVGPEAAGKVDIDAPVAENLAAVARAKGLE
                     LKEVTAVILDRPRNRHYIDAVRAAGARVRLISDGDVAPALMTALPDTGIDIVIGSGGT
                     PEGVITACAMRGLGGEIQGRLLPQSDYERERVEAAGFDIQRKLLLTDLASGDDHFFAA
                     TGITQGDFLRGVRYTATGAVTHSLVTRSRSGTWRTIEAHHRWDKLMSYSNVEYHS"
     gene            complement(5340..6446)
                     /locus_tag="PLC06_03580"
     CDS             complement(5340..6446)
                     /locus_tag="PLC06_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRO89556.1"
                     /translation="MSASTEIQIIAVIVAIACALPGTFLVLRRMAMMSDAISHTVLLG
                     IVLGYFLLGSLESPFLILAAAAMGVVTVSLTELLMRTRLVRQDAAIGLVFPALFSIAV
                     ILISRFARGVHLDVDAVLLGELAFAPFDRIYFLGYDLPRSMVVMGVTLLANTTLIALF
                     YKELKLTTFDAGLAVALGFSPTLIHYGLMLMVSVTAVAAFDAVGSVLVVALMITPPAA
                     AYLLTNRLSRMLLYSAALAAAGALAGFWMAWFLDANIAGSIATMLGMLFLGVFLLAPE
                     RGLVAQAARRGRQRWEFAQTALAIHLLNHEATPQMESECRVDHLHYHMRWEPNFAVAV
                     VDLAERAGLVLRHDGDLSLTPQGRERARTALVHA"
     gene            complement(6534..7667)
                     /locus_tag="PLC06_03585"
     CDS             complement(6534..7667)
                     /locus_tag="PLC06_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRO89557.1"
                     /translation="MGELINTLLFDYTVRTVAMGAAVLGIVAGALGTFALLRRQSLLG
                     DAMSHAALPGVLLAFMLTGSKAPVVLVLGAAVAGVLGTFLLLSIIRFSRIKEDAGLGI
                     ILSVFFGFGLVLLTFLQRNPTAAQAGLNKFLFGQAATLLVGDVVTMAIFGGGALALLI
                     LFWKEFKLLSFDRDYGSSLGYPMTALDLLLTTLLVIGIVIGLQAVGVVLMSALVVAPA
                     AAARQWTDRLSVMMVVASFFGAIAGVAGAFISSLGTGLSTGPVVVLAISAIVLLSLLF
                     GAGRGLVWTWLQQQRNRRRLRTDAVLAWLYDLGVVQHADPRYAHNVEVLRAMSRYQGG
                     VRNSLRALEVDGLVHRTSPDEWALTDKGVGRARELRTTGRSDS"
     gene            complement(7655..8470)
                     /locus_tag="PLC06_03590"
     CDS             complement(7655..8470)
                     /locus_tag="PLC06_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRO89558.1"
                     /translation="MTDTKYAIEVTDLTVAYQEKPVLWDIDLHVPPGILMAIVGPNGA
                     GKTTLIKAILGLIKPVAGQTLIYGKPYAEQRRLVGYVPQRGSVDWDFPTSVLDVVMMG
                     RYGELGWFKRPRREDRELALEALAKVGMVDFAGRQISQLSGGQQQRTFLARALVQDAQ
                     IYFMDEPFQGVDATTERAIVALLKELRAAGKSVLVVHHDLQTVPEYFDWAFLINVSRI
                     AAGPVETVFTDENLRRTYGGRVGFLTGNGHGDSTVHGDPAAPKPAGEALEWAS"
     gene            complement(8798..9727)
                     /locus_tag="PLC06_03595"
     CDS             complement(8798..9727)
                     /locus_tag="PLC06_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRO89559.1"
                     /translation="MNGSSKLFLLAAILLLAACGHNAAAGQPDDGRIRVVTTTGQVGD
                     IAKNVGGEHVHVTSLMGAGTDPHLYSAGARDVDKLRGADIVFYSGLFLEAQMDDILHQ
                     MSERQTVAAVSANINHAELLPSAKYADEFDPHIWFDVGLWMQATEQVRDTYKAFDPAN
                     AADYDANAAAYLAELEALDAYVRDQAATVPAEQRVLVTAHDAFNYFGRAYGFEVLGLQ
                     GISTASEAGTADVQRLADTITERRIPAIFVESSVPVRNIEAVQAAVRNRGFDVAIGGK
                     LFSDAMGDNGTPEGTYIGMVRYNIDTIVSALRQ"
     gene            9873..11444
                     /locus_tag="PLC06_03600"
     CDS             9873..11444
                     /locus_tag="PLC06_03600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRO89560.1"
                     /translation="MDALILAIDLGTSGPKVGLITPQGVVVDCEIEATKLNILPGGGA
                     EQDPGDWWAAIKTAMARLLGNNPDAVGKIKAIGVTSQWSGTVAVDRDGRHLMNAIIWM
                     DSRGAPYAQQVTGGFPKVEGYGLSRLLTWVRLTGGIPTQSGKDPIAHILYIKHRLPDV
                     YRHTHQFLEPKDYLNLRLTGQYASSYETITLHWVTDNRDINAIRYDEKLLSWSTIDRE
                     KLPPLKRAVDVLGPIRPEVARELGLPEDIPVIMGTPDIHSAAIGSGAVADFEPHLYIG
                     TSSWLTCHVPYKKTDLFHNMASLPSAVPGRYFLINEQETAGACLAFLRDNILYADDEL
                     RRDTRVENVYPIFDRMVEATPPGDDKVIFTPWLHGERTPVDDRHVRGGFHNLSLRTTR
                     AHMIRSVFEGVAFNSRWLLQYVEKFTGRRMERIRIIGGGANSNVWCQIIAGVMNRTIV
                     QMREPIQANLRGVGLLAAVATGHTTFAQINNDVPVAAEFTPDPANRAIYDELYAAYSE
                     IYKQNKRIFAKLNQS"
     gene            11447..12958
                     /locus_tag="PLC06_03605"
     CDS             11447..12958
                     /locus_tag="PLC06_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRO89561.1"
                     /translation="MSLKTQFKRIQTRLMPSVEKSLRAIPAVNRRIESQFDELMPELE
                     KSLKPYRDELPGRAQLPETGIERAAVLREIEALREREAARWVDGFVSGAVYHGDEGHI
                     EFLNSIYALTSQANPLHADVWPSIAKYEAEIVAMTADMLGAGSVESDRAGDQICGTVT
                     SGGTESILLAMKTYRDRARETKGITRPEMVVPVTAHAAFDKAAQYFNIEMVRVPVDDQ
                     YRADWRAARDAITKNTIVVVGSAPSFPHGVIDPIAEMSAVALEHGIGFHTDGCLGGFL
                     LPWAADLGYPVPPFDFRLPGVTSMSADTHKYGYAAKGTSVVLYRGADLRHYQYFTITD
                     WPGGLYFSPTFAGSRPGALSAACWGAMVSLGRDGYRDAARRILETAAQIRAGIEAIPG
                     LRLLGHSYFVHAFTSDEFDIYRVLDQMSARHWSLNGLHHPSCVHVCVTLRHTQPGVAE
                     RFLADLAASVDYVRANPADGGGMAPVYGMAATLPLRGVVSDILKRYIDVLYKV"
     gene            complement(13154..15283)
                     /locus_tag="PLC06_03610"
     CDS             complement(13154..15283)
                     /locus_tag="PLC06_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRO89562.1"
                     /translation="MNWTITQKPSFLDDFIELPKDLQKSVGSAIADMTADPITPRGDT
                     IKKLQGYTNVYRYRIGDFRLIYAADQAAHMANLLRVGPRGAIYQRFNYDGWDAPDAAV
                     DFGPELIDGMGRPNRPEWPTVTKKTTEEEKLPRALTAELLSRWRIDPAHHAALVACRT
                     ADDLLGAGDRIPPAAIERVMKGLYEVDVDELAAQPDLMLFDPEDLARFAEGDLAHFLL
                     RLDDRQQPLTQWALQGPTLVKGGPGSGKSTVALYRVRAVVRHHLDTTGHLPSVLFTTY
                     TKALINASKSLLRQLLGKSVQLTKKGELPNEFRISTHHATATMILKGSGLNYNMANNY
                     QTAEALHAARATLTPRTFGQADLIDVHSRLKELRDDYLEEEFNWVIEGQNCRTAEDYF
                     RADRSGRGISFTRERRAAVWRLYEAFRDHLAGQNLITWGQLTLLALDRVRAGEFSRRW
                     DYVIVDEAQDLPPAALALAVELCREPGGVFLTADANQSIYNRGFRWSSVHDSLRVAGR
                     ARTLRRNYRTSRPIATAAAEIVADIAGTDGEAAEQEYIHTGPRPTLYAASGSTEQWRY
                     IAHWIVDAARRLRLPAAAAAVLVPSSEVGTLLADALCDHGLPAKFMNSQAFDLDEPGV
                     KVTTLHAAKGLEFPIVIVAHVEAGRLPRPTDATDPDEIAAYEASQRRLFYVGCTRAMR
                     LLLVTYDRPLPSPFLVELSEEMWERVE"
     gene            complement(15438..>16327)
                     /locus_tag="PLC06_03615"
     CDS             complement(15438..>16327)
                     /locus_tag="PLC06_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRO89563.1"
                     /translation="WEQWIPADGRFPVNARAVRSTLQSERSCQAIVKKAVAERLMVAH
                     GVAALPETGADYGIQVTIRQDRATLALDTSGDGLHKRGYRAEAGAAPLTETLAAALVL
                     LSFWSKGRLLIDPMCGSGTILIEAAMIGRRMAPGLRRSFAAERWPAVGEGAWPAARHE
                     ATEAVTPSETPALFGYDIDADAIRIAKQNAALAGVGEDITFEQKALADLWIDQEYGIL
                     ISNPPYGQRMAEFREINDLYITLNKMMRKKRGWSVYILTADAKFPDYFKRGRPDRVRK
                     LFNGNIRVDYYQYYGERGE"
BASE COUNT         2994 a         5222 c         5190 g         2921 t
ORIGIN      
        1 gcccacgaac acccccggtc cagacccgac cgccaccccg acatcgacgc cgccggcggc
       61 cggtatgcat atcggcgacc tggacggcag ctccgtctac accaacaaca acaaccgctg
      121 gaaggcgtcg gtaacgttca ccgtccacaa cggctcgcat ggtgtcgtca gcggagccag
      181 ggtgaccggg aaatggtcca acggcgcgag tggcaacgcc agctgcacca ccaacgccgc
      241 agggcaatgc tcgatcaata agcccaaact gctgacgaac gtgtccagtg tgacttttac
      301 tgtgacgaag gtgacccgga gcgggcagac ctatagcgcc gcctccaacc acgatcctga
      361 cggcgacagc aacgggacct acattgttgt gcaaaagccg taacagaaat tcaggcgact
      421 actgactgcg aatgttcgta gccagacgct ttagcgccgt tccgggaacg caactaaagc
      481 tgccggctgc gaacctggag cacaattgat gaaagaagcc cgccagactc gtgatatcga
      541 gcctggcggg cttctttatc tttattgttg tggtgtggcg gccaggtaac ttgccgggcc
      601 tatgatctgc ttccacattt ggtacaatgt ccgtgcaagg agatttgcgc cgaggcgcca
      661 tgcagatagc atggcggccg aaaccgaaag cacgccatgg cccaaaaaat cattcgctgc
      721 accgaccgcc atttcgagcg catgctggag ttcgtcggcg cccataaccc ggcgagcgag
      781 caccacatcg gctatttcgg cgtgacgccg gccgatattc gccaatctgt cctgatgctc
      841 aatcttcgtt acaatcgggg cttccgcctg gcgctggagg gcagccggct cgtcggcgtc
      901 atgggcatgg actttgacaa ggagatcggg cgagcctggc tctatggccc catcgtcgtc
      961 gccgacggat cggctgccga ctggacggcc acggccgacg cgctctatgc cggcgtcacg
     1021 ccggttttgc cggtgggcat ccatgagcac gagatgttcg ttgacacgga gaatgcccat
     1081 tgtcgtgctt tcgccgcgcg gcatgatttc cacaatcagg gcgagatgga gatctatttc
     1141 atcacgcccg accgattggc agctttgccg gaggcgacgg ccgaggaatg ggacggccgc
     1201 ttcgccgacc ggttcatcgc gctccacgat caacttttcc cgcgctccaa cttcacgctc
     1261 aactctatgc tcagcgagcg agacaagggc gctacattgc tgttgctgac cgacggcgat
     1321 gatctggcgg gctacttctt cggccgcgcc gacgcggtgt cgggcgaggc ctttgtggac
     1381 ttcgtcggcg tcgatccggc ccgccatggc cagggcctgg gacgacggct catgctggcc
     1441 gggctggcgc gtcagcgggt gacgcccaat ctccatcagg tgaatctgac cgtgaagccc
     1501 gataatctcc ccgctatgcg cctgtacgag tcactgggtt tcgtccgcga gcgcggtatg
     1561 gtggcatttc gcaagaaagt ggggcaagat tgaagccgat atcgctaaca tgtgaacagg
     1621 gaatggaaac tcatgatccc caagaaaatt ctcatcctga ccgccgacgc cggattcggc
     1681 catcgcagca cggccaatgc cttgtgccgg gctttcgaac tgcgctacgg cgaggcggcc
     1741 caggtgatca tcgtcaatcc gctggacgac gtgacggccc tcttttttct acgcagcgcc
     1801 gaaaacgact atgacaagct ggcccgcgaa tggccgcagt tctacaagct gggctaccgg
     1861 atgatcgacc tcagcctgac gaccaacgtc actgaaatgg cctacgtgat gatgatgtat
     1921 gatccgatct cgcggctcat gaagcagcac gagccggacg tggtggtcat caccttcccg
     1981 acgtatcaat atcccgtggc cgcctatcgg cgcatcaccg gcgcgcgcgt gcccatcgtc
     2041 accgtcgtga ccgatctcgt atccttgcaa aagatgtggt tcagccagtc ggtggatatg
     2101 tgcctcgtcc ccacgaggga agcggccgcg gcggccacag agcgaaacat ggaccccgaa
     2161 agcatccaca tcaccggttt gccggtcaac cccgcgctgg cggccgttcc cgcgtcgaag
     2221 acggaagctc gccgcgcgct caactggccg gaggacaagt tcaccgtgtt ggccgtgggc
     2281 agcaagcgcg tcgaagctct ggagacgttc gccaacgtcc ttaaccattc cggccacgat
     2341 ttgcatttcg tgggcgtggc cggcggcaat gaccagctct acacggcgct ccgggacgct
     2401 gaatggcata tcccggcgac tatctacgac tacatcacca atatgcccga tctgatgcat
     2461 gctgccgact gcatcgtctc caaggccggc gggctgatcg tcaccgagtc gctggcgtgc
     2521 gggctgccca tgctcattac ccaggtcatc cccggccagg agaccggcaa cgccaaatat
     2581 atggtcgacg gtggcgcggg cgatctgacg atggagccgc tccagtttct ggagacgatg
     2641 actcactggc tggccgatga tcggcggctg tataaggagc gcgcggccaa tgcccgtcgt
     2701 ctggggcggc cgggcgcggc cttcgctgcc gcagacctta tttgggattt gaccaaggcc
     2761 ggcgcggcca ggcaccgcag tcgcctgccc gtctcccgcg aaaagctcag ggagctatta
     2821 cgacaattca gcggcacgga gaagccggtg ccctcgtgat tgatggcgag ttagcggccg
     2881 ggagggaaca tcggccgagg attgttcgtc ccatctacag gagttcaccc gtgaactccg
     2941 tggaggtttg aagatgcgca agttggtgac tttgtggttg gtgacgctgc tgctattggc
     3001 ggcttgcagt gccggcggag ccaataatga gggacaggcg accgggacgc acgatgacgt
     3061 ggcggctatc accctgatca cccccgaatc gggcgggggc tatcccgcgc ctgccaatcc
     3121 ctatcctgtt tcgccggtcg aggccctttt gcccacggat tatcctggaa tggctatcag
     3181 caccatgccg ccgaccgacc tgacgcccga cgcgggcgcc aattcagcgg cgacatcgcc
     3241 cccgaccgat cggccgctcg ccacgccgta tcccgtgccg gaccgggtgt tggccacact
     3301 cgtccgcgaa ctgtcggcgc tggccgacgt gccgatcgat gagatcgatg ttgccgcggc
     3361 cgaaccggtg gtgtggccca atgccggcct cggttgcccc gccgaaggga tggcctatgc
     3421 cgaagttcag gttgagggga tgctgatcgt gctggccgcg ggcgggcgcg attatacgtt
     3481 ccacaccgac ggcgcgcagc gctatgtcct ttgccgggat gggcggccaa tatccgaggg
     3541 caacatcaag gatcgcggct gaagtcgctt gctacgagcc gttggatagc gccgcccgga
     3601 acgcggcgat gttcccagcc actgtttggg aaccgccaaa gatcgcgctg ccggccacca
     3661 gcgccgacgc gcccgcagcg acgatctccg cggcgttgtc gggcttgatg cccccgtcca
     3721 cttccagctc ggccgtagag ccgatgaagt ccagtatcgc ccgcagccgc cgaattttat
     3781 cggtgctggt ggggatgtag gattggccgc cgaaccccgg attgaccgac atgatcagca
     3841 ccagatcgac gtagggtaat atctcttcga gggcgaccag cggcgttgcc gggttgagcg
     3901 tgacgccggg gcgtgcgccc acctcgcgga tcgcctcgac ggtgcggtgc aggtgggggc
     3961 aggtctcgac gtggacagtg aggatatccg ccccggcgcg cgcaaaatcg gccagatagc
     4021 gttccggctt ctcgatcatc aggtggacat cgaccgtcgc cccgtagcgc cgggcgatgg
     4081 gagccacggc gcgcacgatc ggcgggccga tggtgatgtt gggcacgaaa tggccgtcca
     4141 tcacatccac gtgaatatag tcggcccccg ccgccagcgc ttcctccacc tgctcgccga
     4201 ggcggctgaa atcggccgac aggatcgatg gagccagccg aatcggcctc atgaatggta
     4261 ctccacgttg ctgtagctca tcagcttgtc ccagcggtgg tgggcctcga tggtgcgcca
     4321 cgtgccggag cgggatcgcg tcaccagcga atgggtgacg gcgccggtgg ccgtgtagcg
     4381 cacgccgcgc aggaagtccc cctgggtgat gcctgtggcg gcaaagaaat gatcgtcgcc
     4441 ggaagccagg tcggtcagca ggagcttgcg ttgaatgtcg aagcccgccg cctcgacccg
     4501 ctcccgttca tagtcggact ggggcaatag ccgtccctga atctccccgc ccaggccgcg
     4561 catggcgcag gcggtgatca ccccttcggg cgtgccgccg ctgccgatga cgatgtcgat
     4621 cccggtgtcg ggcagggcgg tcatcagcgc gggggccacg tcgccgtcgc tgatgaggcg
     4681 cacgcgagcg cccgcggcgc gcacggcgtc gatataatgc cggttgcgcg gccgatcgag
     4741 gatgaccgcc gtcacttcct taagctccag tcccttggct ctggctacgg ccgccagatt
     4801 ctcggccact ggagcatcga tatcgacctt acccgccgcc tcagggccaa cgaccagctt
     4861 gtccatgtag aaggccgggc cgggattgaa cagcgtgcct cgcgcggcga tggcgacgac
     4921 ggagatggag ttgggcagcc ccttggccag caggttcgtc ccttcgaccg gatccacggc
     4981 gacatcgacc tgcgggccgc ggccgttgcc gacgatttca ccgttgtaga gcatgggcgc
     5041 ttcgtctttc tcgccctcgc cgatgatgac gatgccgtcc atgcgtaccg accccagcag
     5101 gagccgcagc gcgtcgacgg ccgcgccgtc tccctcttcc ttctgcccgc ggccaaccca
     5161 tcgcgcggcg gcgagcgcgg cggcttcggt cgcccgcacc agttccagcg ccagattccg
     5221 atcgggagct tccgacattg acatgttctg cctcctggag aatgttattg ggtgcgggga
     5281 gtatacccgg ttatgaatgt gtggcctatt tattgaggtc taaaacatcc cccgccgcat
     5341 cacgcatgaa ccagcgccgt ccgcgctcgt tctcgcccct gcggcgtcaa tgacaggtcg
     5401 ccgtcgtggc gcagcaccag accggcgcgc tcggccagat ccaccaccgc tacggcgaag
     5461 ttcggctccc agcgcatatg ataatgcagg tgatcgaccc ggcactcgct ctccatctgc
     5521 ggcgtggcct cgtggttcag caggtggatg gccaatgccg tctgggcgaa ctcccaacgc
     5581 tgacggccgc gccgcgccgc ctgtgccacc agcccgcgct ccggggcgag caggaacacg
     5641 cccagaaaca gcatgcccag cattgtggcg atggaaccgg caatgttggc gtcgaggaac
     5701 caggccatcc agaaaccggc gagggccccg gccgcggcca gcgcggcgct gtagagcagc
     5761 atccggctga gccggttggt cagcagatag gcggccgcgg gcggggtaat catcagggcc
     5821 accaccagaa ccgaccccac cgcgtcgaag gcggccacag ccgtgactga caccatgagc
     5881 atcagcccgt agtggatcag ggtgggggag aagcccaggg cgaccgccag cccggcgtcg
     5941 aacgtcgtca gtttcagttc cttgtagaac agagcgatga gggtcgtgtt ggcgaggagc
     6001 gtgacgccca tgaccaccat gctgcgcggc aagtcgtagc ccaggaaata gatgcggtcg
     6061 aacggcgcga acgccagttc gcccagcagc accgcgtcga cgtcgagatg gacgccgcgg
     6121 gcgaagcggg agatcaggat gacggcgatg ctgaacaggg cggggaacac cagcccgatg
     6181 gccgcgtcct gccgcaccag gcgcgtgcgc atcagcagct cggtcaggct gacggtgacc
     6241 acgcccatgg cggcggccgc cagaatcagg aagggcgact ccaggctgcc gagcaggaag
     6301 tagcccagga cgatgcctag cagcacggtg tggctgatgg cgtcgctcat catggccatc
     6361 cggcgcaaaa cgaggaacgt tcccggcagg gcgcaggcga tggcgacgat gacggcgatg
     6421 atttggattt cagtcgatgc actcataatt tatgttgcgt ggccacggtt agtagtttat
     6481 ttcgtgtagc cattagtcaa tatcatccaa acaatccaac caaaagggtg gagctatgaa
     6541 tcgctccggc cagtggtgcg cagctcgcgc gcgcggccga cgcctttgtc tgtcagcgcc
     6601 cactcgtcgg gggaagtccg atgcaccagc ccgtccactt ccaatgcccg caaactgttg
     6661 cggacgccgc cctgataacg gctcatggcc cgcagcacct ccacattgtg ggcatagcgc
     6721 gggtcggcgt gctgcacaac ccccagatca tagagccagg ccagaacggc atcggtgcgc
     6781 agccggcgac gattgcgctg ctgctgcagc caggtccaga ccagcccgcg cccggccccg
     6841 aacagcagcg acagcaggac gatcgccgag atggccagca ccaccaccgg cccggtggac
     6901 agccccgtgc cgaggctgct gatgaacgcg ccggccaccc cggcaatcgc tccgaagaac
     6961 gaggcgacga ccatcatcac gctcaggcga tcggtccatt gacgcgcggc ggccgccggg
     7021 gcgacgacga gcgcgctcat cagcaccacg cccacggcct gcaagccgat gacgatgccg
     7081 atgaccagca gcgtcgtgag gagcaagtcg agggcggtca ttggatagcc caggcttgag
     7141 ccgtagtcgc ggtcgaagct gagcagcttg aactccttcc agaacaggat gagcagcgcc
     7201 agcgccccgc cgccgaagat ggccatcgtc acgacgtcgc cgaccagcag cgtggccgcc
     7261 tggccaaaca ggaacttgtt gagacccgcc tgtgccgccg tcggattgcg ctgcaggaat
     7321 gtgagcagca ccaggccgaa gccgaagaaa accgacagaa tgatgcccag cccggcgtct
     7381 tccttgatgc ggctgaagcg aatgatcgac agcagcagga aggtgcccag caccccggcc
     7441 acggccgcgc ccagcaccag caccaccggc gccttgctgc cggtcagcat gaaggccagc
     7501 agcacgcccg gcagcgcagc atgtgacatg gcgtcgccca gcaggctctg gcggcgcagc
     7561 agggcgaacg tgcccagcgc cccggcgacg atgcccagca cggccgcgcc catcgccacc
     7621 gtgcgcacgg tgtagtcgaa cagcagggtg ttgattagct cgcccattcc agcgcctctc
     7681 cagcgggttt gggcgcggcg gggtcgccgt ggacggtaga gtcaccgtgg ccgttgccgg
     7741 tcaggaaacc gacccgtccg ccgtaggttc gccgcaggtt ctcgtcggtg aacaccgtct
     7801 ctaccggtcc ggcggcgatg cggctcacgt tgatcagaaa cgcccaatcg aaatattccg
     7861 gcacggtctg caagtcatga tggacgacca gcaccgattt gcctgccgcg cgcagctcct
     7921 tcagcagggc gacgatggcc cgctcggtgg tggcgtccac accctgaaac ggctcgtcca
     7981 tgaagtagat ctgcgcgtcc tgaaccagcg cgcgggccag aaaggtacgc tgctgctggc
     8041 cacccgaaag ctggctgatt tgccgcccgg cgaaatcgac catgccgacc ttggccaggg
     8101 cttccagcgc cagttcgcgg tcctcgcgac gcggccgttt gaaccagccc agctccccgt
     8161 agcggcccat catcaccacg tcgagcacgc tggtggggaa gtcccagtcc acgctgccgc
     8221 gctgcggcac atagccgacc agccgccgct gctcggcgta gggcttgcca tagatgagcg
     8281 tctgcccggc gacgggcttg atcagcccca ggatcgcctt gatcagcgtc gtcttgcccg
     8341 cgccgttggg gccgacgatg gccatgagga tgcccggcgg cacgtggaga tcgatgtccc
     8401 acaggacggg cttttcctga taggcgaccg tcagatcggt cacctcaatc gcatattttg
     8461 tatcagtcat aattgcctca aatgaagata ttcattcgct tttgttcgta gtaggcgctt
     8521 cagcgccgct cttcgttcgt agtcaggcgc ttcagcgccg ctcttcgttc gtagtcaggc
     8581 gcttttggat atttatcaat taatgcggtg tagcccggtt gattgatcct ccgggtgtgg
     8641 cgcaatctga cagattacgc tacaacgcta taccgcatta atttgttaac atgcaacagc
     8701 gccgctcttc gttcgtagtc aggcgcttca gcgccgctct gggggaacgc agctaaagct
     8761 gcttactacg aacaaagaag ccttgcgtta ggatgtatta ttgccggagc gccgacacga
     8821 tggtatcgat gttgtagcgc accatgccga tgtaggtacc ttcgggtgtg ccgttgtcgc
     8881 ccatagcgtc ggaaaagagc ttgccgccga tggccacgtc gaagccgcga ttgcgcacgg
     8941 ccgcctgcac cgcctcgatg ttgcgcaccg gcaccgacga ctcgacgaag atggccggga
     9001 tgcgccgctc ggtgatagtg tcggccaggc gctgcacgtc ggccgtgccc gcttccgacg
     9061 cggtggagat accctgcaag cccagcacct cgaagccata ggcgcggccg aagtagttga
     9121 aggcgtcgtg ggcggtcacc agcacgcgct gctcggcagg cacggtggcg gcctgatcgc
     9181 gcacgtaggc gtcgagtgct tccagctcgg ccagataggc ggcggcgttg gcgtcgtagt
     9241 cggcggcatt ggccgggtca aacgccttgt aggtgtcgcg cacctgctcg gtggcctgca
     9301 tccacagccc cacgtcaaac cagatgtgag ggtcgaactc gtcggcgtat ttggccgagg
     9361 gcagcagttc ggcgtggttg atattggccg acacggcggc caccgtctgc cgctcgctca
     9421 tctggtgcag gatgtcgtcc atctgcgcct ccagaaagag gccgctgtag aagacgatgt
     9481 cggccccgcg cagtttgtcg acgtcgcggg cgccggccga atagagatgg gggtcggtgc
     9541 ccgcgcccat caggctggtc acatggacat gctcgccgcc aacgtttttg gcgatgtcgc
     9601 ccacctgacc ggtggtcgtc accacacgga tgcggccgtc gtcgggctgg ccggcggccg
     9661 cgttgtggcc gcaggccgcc agcagcagga tcgccgccag gagaaacaat ttagatgaac
     9721 cattcataat aaccatcact aatttagata cgtctaaatt atataggata gttggatggt
     9781 gtcaatgtga aatggtgggg tgggggcgcg gctccgttgc catctacccg atatgcgcta
     9841 ccatggtaca tctttttttg gggagacaag tcatggatgc gcttattctg gccatagatc
     9901 tggggacatc cggcccaaaa gtcggtttga tcacgccgca gggggtcgtc gtcgattgcg
     9961 agatcgaggc gacgaagctc aacatattgc ccggcggcgg ggcggaacag gaccccggcg
    10021 actggtgggc ggcgatcaaa acggccatgg cgcgcttgct gggcaacaat cccgacgccg
    10081 tcgggaagat caaggccatc ggcgtcacca gccagtggtc gggcacggtt gccgtcgacc
    10141 gcgacggccg ccatctgatg aacgccatca tctggatgga ctcgcgcggc gcgccctatg
    10201 cccaacaggt caccggcggc ttccccaagg tcgagggcta tggcctctcg cggctgctca
    10261 cctgggtgcg cctcaccggc ggcattccga cccaatcggg caaggacccc atcgcccaca
    10321 tcctctacat caaacaccgg ctgcccgacg tctatcgcca cacgcaccag ttcctggagc
    10381 cgaaggacta cctgaacctg cggctgaccg gccaatatgc ctcgtcgtat gagacgatca
    10441 ccctccattg ggtgaccgac aaccgcgaca tcaacgcgat tcgctacgac gagaagctgt
    10501 tgagctggtc gaccatcgac agggagaagc tgccgccgct caagcgcgcc gtcgatgtcc
    10561 tcggcccaat ccggccggag gtggcccgcg agctggggct gccggaggac atccccgtca
    10621 tcatgggcac gcccgacatc cattcggcgg ccatcggctc cggcgcggta gccgattttg
    10681 agccgcatct ctacatcggc acatcctcct ggctcacctg ccacgtgccc tacaagaaga
    10741 ccgacctgtt ccacaacatg gcctcgctgc cgtcggccgt tccggggcgc tatttcctca
    10801 tcaacgagca ggagacggcc ggggcatgcc tggccttcct gcgcgacaat atcctctacg
    10861 ccgacgacga gttgaggcgc gacacgcggg tggaaaacgt ctatccgatc ttcgaccgca
    10921 tggtggaggc cacgccgccc ggcgacgaca aggtcatctt taccccctgg ctccacggcg
    10981 agcggacacc ggtcgacgac cggcatgtgc gcggcggttt ccataacctg tcgctgcgca
    11041 ccacgcgcgc ccacatgatc cgttcggtgt tcgagggggt ggccttcaac tcgcgctggc
    11101 tgctgcaata cgtggagaag ttcaccgggc ggcgcatgga gcgcattcgc atcatcggcg
    11161 gcggtgccaa ttccaatgtc tggtgccaaa ttatcgccgg cgtgatgaac cggacgatcg
    11221 tccagatgcg cgagccgata caggccaacc tgcgtggggt ggggctgctg gcggccgtgg
    11281 ccacaggcca taccaccttc gcccaaatca acaacgacgt gcccgtggcg gccgaattta
    11341 cgcccgatcc ggccaatcgc gccatctacg acgagctgta cgccgcctat agcgaaatct
    11401 acaagcagaa caagcgaatc tttgccaaac taaaccagag ttgacgatgt ctctaaaaac
    11461 ccaattcaaa cgaattcaga cgcggctcat gccgtcggtc gagaaaagcc tgcgcgccat
    11521 tccggcggtc aatcggcgca tcgagtccca gttcgacgag ctaatgcccg aactggagaa
    11581 atcgcttaaa ccgtatcgcg acgagctgcc ggggcgcgcg cagttgccgg agacgggtat
    11641 tgagcgcgcg gccgtcctgc gggagatcga ggcgctgcgc gagcgagagg cggcgcgctg
    11701 ggtcgacggc ttcgtgtccg gcgcggtcta ccatggcgac gagggccata tcgagttcct
    11761 gaacagcatc tacgccctca catcgcaagc caatccgctc cacgccgacg tctggccgag
    11821 catcgccaag tatgaggccg agattgtggc catgacggcc gacatgctgg gcgcgggttc
    11881 agttgagtcc gatcgcgccg gagatcaaat ctgcggcacg gtgacgagcg gtggcacgga
    11941 gagcatcctg ctggccatga agacctaccg cgaccgcgcg cgcgagacga agggcatcac
    12001 ccggccggag atggtcgtgc cggtgacggc ccacgcggcg ttcgacaagg cggcccaata
    12061 tttcaacatc gagatggtgc gcgtgccggt cgatgaccaa taccgggccg actggcgggc
    12121 ggcgcgcgac gcgatcacca aaaataccat tgtggttgtc ggctcggctc cgtcgtttcc
    12181 ccatggcgtc atcgacccca tcgccgagat gtcggccgtg gcgctggagc acggcatcgg
    12241 cttccacacc gacggctgcc tgggcgggtt cctgctgccc tgggcggctg atctgggcta
    12301 cccggtgccg ccgttcgatt tccggctgcc gggcgtcacc tcaatgtcgg ccgacaccca
    12361 caagtacggt tacgccgcca agggcacgtc agttgtcctc tatcgcgggg ccgacctgcg
    12421 ccattaccaa tatttcacca tcaccgactg gccgggcggc ctgtatttct cgccgacctt
    12481 cgccggcagc cggccgggcg cgctcagcgc cgcgtgctgg ggggcgatgg tgtcgctggg
    12541 gcgcgacggc taccgcgacg ccgcgcggcg catcctggag acggccgcgc aaatccgcgc
    12601 gggcatcgaa gccatccccg ggctgcgtct gctgggccat tcatacttcg tccacgcttt
    12661 cacatccgac gagttcgaca tctaccgcgt gctggatcag atgtcggcgc ggcactggag
    12721 cctcaacggg ctgcatcatc catcgtgtgt ccacgtgtgc gtcacgctgc gccacacgca
    12781 gccgggcgtg gccgagcgat tcctggctga tctggcggcg tcggtcgatt atgtgcgcgc
    12841 caatccggcc gacggcggcg gcatggcgcc ggtctacggc atggcggcga cattgccgct
    12901 gcgcggcgtg gtcagcgata ttctgaagcg gtatattgac gttttgtata aagtgtaact
    12961 aataaacctt agtttaacta taaggaattg agggtggggt tgctgctcga catcgaccac
    13021 cactgccctt ctcaaaaact tgcaggacgg cgtgcagaaa ttactgtttc tatgtgccaa
    13081 ttttatggca cagtgtcctg gttggaggga ttgactgaag aggcgctgaa gtaagcgact
    13141 taaaatctat ccatcattca accctctccc acatctcctc actcaactcg accaaaaacg
    13201 gcgacggcaa aggacgatca tacgtcacca gcagcagccg catggctcgc gtacaaccga
    13261 cgtagaatag ccgccgctgg ctcgcctcgt aggcggcgat ctcgtccggg tcggtagcat
    13321 ccgtggggcg cggcagccgc ccggcttcca cgtgggccac gatgacgatg gggaactcaa
    13381 gccccttggc cgcgtggagc gtcgtcacct tgacgcccgg ctcatccaga tcaaacgcct
    13441 ggctgttcat aaatttggcc ggcaggccat ggtcgcacaa ggcgtcggcc aatagtgtgc
    13501 ctacctcgct ggacggtacg agaacggccg ccgccgccgc cggcagtcgc aggcggcgcg
    13561 ccgcgtcaac gatccagtgg gcgatgtagc gccattgctc agtcgagccg ctcgccgcat
    13621 aaagcgtggg ccgcggcccg gtgtggatgt actcctgctc ggccgcttcg ccgtctgtgc
    13681 cggcaatatc ggcgacgatt tcggccgcgg cggtggcgat gggtcgggat gtgcggtagt
    13741 tgcggcgcag cgtccgcgca cggccggcca cgcgcaggga gtcgtggacg ctgctccagc
    13801 ggaagccgcg attgtagatc gactggttgg cgtcggctgt caggaacacg ccgcccggtt
    13861 cgcgacataa ctctacggcc aacgccagcg ccgccggcgg caagtcttgc gcctcatcga
    13921 cgatgacgta gtcccagcgg cggctgaatt ccccggcccg cacccggtcg agggcgagca
    13981 aggtcagttg cccccaggtg atcaggttct gcccggccag gtgatcgcga aaggcctcat
    14041 agagccgcca gacggccgcg cgccgttcgc gggtgaagga tatgccccgc ccggagcggt
    14101 cggcgcggaa gtagtcttcg gccgtgcggc aattctggcc ctcgatgacc caattaaact
    14161 cctcttccag gtagtcgtcg cgtaactcct tgagccggct gtggacgtcg atcaggtcag
    14221 cctggccgaa ggtgcgcggt gtcagggtgg cccgggcagc atgaagcgct tcggccgttt
    14281 gatagttgtt ggccatgtta tagttcaggc cgctgccttt caggatcatc gtcgccgtgg
    14341 cgtggtgggt gctgatgcgg aactcgttgg gcaactcacc ttttttggtc agctggacgc
    14401 ttttgcccag cagttggcgc agcaatgact tgctggcgtt gatgagggct ttggtatagg
    14461 tcgtgaagag caccgacggc agatggccgg ttgtgtcgag gtggtgtcta acgaccgcgc
    14521 gaacgcgata gagggccacg gtcgacttgc cggagccggg gccacccttc accagcgtcg
    14581 gcccttgcag cgcccattgg gtcagcggtt gctggcggtc gtcgagtcgc agcaggaaat
    14641 gggccaggtc gccttcggcg aagcgagcca ggtcttcggg gtcgaaaagc atcaggtcgg
    14701 gctgcgcagc cagttcatca acatcgacct cataaaggcc tttcatcacc cgctcaatgg
    14761 ctgcgggggg aatgcgatcg cccgccccca gcagatcgtc cgcggtgcgg caggcgacga
    14821 gagcggcgtg atgggccgga tcgatgcgcc agcggctgag aagttcggcc gtcaatgcgc
    14881 ggggcagctt ttcctcttcg gttgttttct tcgtaactgt aggccactcc ggccggttgg
    14941 gccgccccat gccatcgatc agctcagggc cgaagtcaac ggccgcatcg ggcgcgtccc
    15001 agccgtcata gttgaaccgc tgatagatag cgccacgcgg gccaacacgc agcaggttcg
    15061 ccatgtgggc cgcctgatcg gcggcgtaga tcaaccggaa atcaccgata cggtagcgat
    15121 agacgttggt atagccctga agctttttga tcgtgtcacc gcgcggggtg attggatcgg
    15181 ccgtcatgtc ggcgatggcg ctgccgacgc tcttttgcaa gtctttgggc agttcgatga
    15241 agtcatcgag aaaggaaggt ttttgggtga tggtccaatt cataatctat caatcagcgg
    15301 tcagtccatg ccgaagttgg tgaacaatat caggttggtg gtcgccgaac atgggcgatg
    15361 gggacaggtg ctcgcaaccg tcattagttt atgacggcac gcttacgatg caaatgacgg
    15421 cactccataa caccccccta ctcccctctc tccccataat actgataata atccacccga
    15481 atattgccgt tgaacagctt gcgcacgcga tccgggcggc cgcgcttgaa ataatcgggg
    15541 aatttcgcgt cggccgtcag gatgtagacc gaccagcccc gcttcttgcg catcatcttg
    15601 ttgagcgtga tatagagatc gttgatctcg cggaactccg ccatccgctg tccatacggc
    15661 gggttggaga tcaggatgcc gtactcctga tcgatccaca ggtcggccag cgccttctgc
    15721 tcgaaggtga tatcttcccc caccccggcc agcgccgcgt tctgtttggc gatgcggatg
    15781 gcgtcggcgt cgatgtcgta gccgaacagg gccggcgttt ccgatggggt gaccgcttct
    15841 gttgcctcat gacgcgccgc cggccaggcc ccctccccca ctgccggcca gcgctcggcg
    15901 gcgaacgagc gccgcagccc gggggccatc cgccggccga tcatcgccgc ctcgatcagg
    15961 atggtgccgg agccgcacat cgggtcgatc agcagccgcc ccttgctcca gaacgacagc
    16021 agcaccagcg ccgccgccag cgtctcggtc agcggcgcgg cccccgcctc ggcgcgatag
    16081 ccgcgcttgt gcagcccgtc gccggacgtg tccagcgcca gcgtggcccg atcctggcgg
    16141 atggtgacct ggatgccgta atcggccccc gtctccggca gcgccgccac cccgtgggcg
    16201 accatgagtc gctcggccac cgctttcttg acgatggcct ggcagctgcg ctcgctctgc
    16261 agcgtggagc gaacggcgcg ggcgttgacg ggaaagcggc cgtcggcggg aatccactgc
    16321 tcccacg
//
LOCUS       DAOMQG010000034        16316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1550_length_16316_cov_10.355513, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000034 DAOMQG010000000
VERSION     DAOMQG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_16316_cov_10.355513"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLC06_03620"
     CDS             complement(<1..287)
                     /locus_tag="PLC06_03620"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO89564.1"
                     /translation="MPTVPTDHLAYFSGKHVLITGGLGFIGSNLAHRLLDLGAEVLIL
                     DSLIPEYGGNFYNVHGIEDRLRINIADMRDEHGLRYLVQGQDLIFNLAGQV"
     gene            complement(268..1047)
                     /locus_tag="PLC06_03625"
     CDS             complement(268..1047)
                     /locus_tag="PLC06_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89565.1"
                     /translation="MSNPYSISAFFPAYNDAGTISSMVITVLLTLRELTDDYEVIVVN
                     DGSQDHTAMVLDELARIYPDEVRIIHHPQNRGYGGALRTGFYSATKDWIFYTDGDAQY
                     DPRELKDLAALVDDEVDFINGWKIERQDPLHRIIIGRIYQYTIKLAFGLKLKDVDCDF
                     RLMRKEVFEQIELTSDSGVICVELMKKVQDAGFRLKETPVHHFHRAYGKSQFFRFRRL
                     AQVARDLTKLWYRLVWRKEGPRPAADAPNSSYNQSYADRSN"
     gene            1435..3162
                     /locus_tag="PLC06_03630"
     CDS             1435..3162
                     /locus_tag="PLC06_03630"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRO89566.1"
                     /translation="MLADQGYLIEALARVLYPDGRWIGFNNDVEAAAWDTMSALANNC
                     TLFEATLINDGLLARVDILIKRGNVFEIIEIKSRAFDRQKNDERVQQGLPNLFRAPRS
                     ADGIQSEWRPYLEDVAFQTNVLQQIFPDATVIPYLLMPDSSRPCQIDGLHRQFVLRSH
                     STSNDGDDIPYSNYTGEPQLIRRNPLVCRVNVQEEVGILLPGVRQQIKIYLDSLRPAL
                     TRITTPISTHCRSCEFHVAEGPLRGFHECWGKMADVTPHILDLYHVSQAGGKTADKLI
                     SEGKASLFDVPIKSLSRRDGSVGEHARRQRLQIECTRDNREWFSDELGAVLDTLIYPL
                     RFIDFETCAPTIPHYHGMRPYETIAFQWSCHTINAPNAIPQHYEWLQSIDTFPNELFA
                     TTLRNNLGDEGSVLIWSNHEITILRKIQQQLAEREAAGSDVGAWIGRLLESDRIVDLN
                     QVAQKHYLHPRAGGRTSLKVITDAVWQSNPAIRRRLPQYAAETESEPTSPYHVLPPLS
                     IGGRQIAVAEGTGAIMAYYSMMERASANATLEAAQWRDLLLQYCELDTIAMVMVWWHW
                     RKLTGKMSQ"
     gene            complement(3139..4764)
                     /locus_tag="PLC06_03635"
     CDS             complement(3139..4764)
                     /locus_tag="PLC06_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89567.1"
                     /translation="MRMPYVVGPPVRLPTDFFGRQSQTQQFFETLAGTQMQCVSVLGL
                     RRAGKTSFFQYITHPEIMAAYLPDPQRYVMVYVDVSACKTPAEFYDRVYRKLMSNLPR
                     VPSGAARSQSGNDVYDVESLLYEFGERRVVFLMDEFDQLRAADFGADFMTELRALAGI
                     WDFELAYVTASYWDNYRLGKFVGLPPTSPFYNIFYPTPIYLSGLSPAELDDLVKAPAR
                     RVGISADDEDVAFVRYQAGTLPFFVQAVAAIWLTYKAQGKKPDHSAISQRLVSEMWPY
                     FEQWWSNFSDVERDVAVAVAAEKPVSRLAYGEQEIDHAVQRLKNYGILAATGDNLWVD
                     SALFGHWIHEYTGRGKGKDAASSVTTRANGRPSAGPSWSDTAAARRMLQIIADVTAQF
                     SRLPAIYARKSDTELRDHLKLALESGLAPLSSSVALSERNPADLAVVQNGENHVAVSC
                     RTWMGQTALLRNLNSLLSHLDAPQTHAIDVVFVRDQEFGLVAEAVAQAMPRHACYLGF
                     NGRHDGRLDYRFHLNGDPHSEVTVSVLLAHLPG"
     gene            complement(4761..5723)
                     /locus_tag="PLC06_03640"
     CDS             complement(4761..5723)
                     /locus_tag="PLC06_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89568.1"
                     /translation="MDVSLPENSTLLTNPYVVGRPLTGMSTALYVGREEIFDWFDENL
                     AGMGQPNALMLYGRRRIGKTSTLYQLVEGERGRPLREDRPRHLIPVYIDLQRYAGRPT
                     DEWLRRLARDICRRVEVANHDDVASHGEISDETAYATFDRSLDYLERILPDDSLILLA
                     IDEFEQIRDGIESGVLDSQILPFIRSQIQHRTRIAFLLCGSTALLEPFWGPIADLTAR
                     FEIGPLDSRQTEELVRRPVEGVLEVEDTAVELIWQATAGHPFLIQSICHRLVSQANRN
                     HLRSAIRAEDVESVIARMEREGYGNGTGHGLDAVAATRVEEIKL"
     gene            complement(5957..7123)
                     /locus_tag="PLC06_03645"
     CDS             complement(5957..7123)
                     /locus_tag="PLC06_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO89569.1"
                     /translation="MADRKGAVIGFDVTAALTQGGGIGRYTRELIHALTIEAPQLTYK
                     LFSARPPTKPPIPDSLPVAPHVRHYPAPVDEHWLYRLWYRARLPVPVQTFTGSIDLFH
                     SPDFVLPPVSGRIPTILTVHDLSFVHYPETFPAKLVAYLNRVVPWSVGRAGHILADSL
                     STSRDLQSLWGVAAERITVLYSGVNERFRPVDGATLSAVRRRYGLDRPYVLAVGTVQP
                     RKNYEMLVRAFQPVAERHPHLLVIVGGRGWLDEGLPAEIERLGLGDRVKLAGFVDDAD
                     LPAVYSAADLLVFPSLYEGFGLPLLEAMACGTPVISSNASSLPEVATETSAILLSPHD
                     EKAWTTTILRLLEDEHARDELRMAGLARSAQFSWLATARQLAGLYASLLAGENF"
     gene            complement(7123..7677)
                     /locus_tag="PLC06_03650"
     CDS             complement(7123..7677)
                     /locus_tag="PLC06_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRO89570.1"
                     /translation="MITYVAGVSSRFGFNPRETERFIKFLVVGGIGFVVDFGIFNLLL
                     DPANALVMPGTGFYNLLTQFGFSNYLITHLGPTIASAVSFVAAVLSNFLWNRYWTYPD
                     SRSRSRRRQFTMFFIVSLVGVLIRIPIVFFMTPVFRSLVNNIGALAAYTTRLADNLSL
                     AVAVLVVLFWNFFANRHWTYNDIE"
     gene            complement(7693..9159)
                     /locus_tag="PLC06_03655"
     CDS             complement(7693..9159)
                     /locus_tag="PLC06_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO89571.1"
                     /translation="MISRLPLPIAAGLVGIGAMFILALINPLFGLGLALLLGPFGALE
                     AIVLGPSLFDSGQVALMLTFAAWIGTGLARRRLLIPRTGFNIPWLLFILIAALSLLDA
                     YSVSLGLVELLKWLEIGLIVWLILDMASDPERRPSGYAAVMRLILAMLLLVGLLQALI
                     GIWQFGLRGNGPEHFLVLGRFYRAYGTFEQPNPFGGYMNLIAFPALGLLLGLLVAWWS
                     RRQGEQMADHLTPRGLWLGLALLASVAGSLAVILSWSRGAWLGFLAGAAVLALFSTRR
                     IALGLMVMITALALTIGGLSLGVATGFGPSQAIIDRLSGFREEFTLGDVRGVDINDAN
                     YSVIERLAHWQAAADMARDDLWTGVGFGNYGAAYGDYDLINFPDALGHAHNYYLNLLA
                     EVGLPGVLAYVLFWLVLVGQSVRYAQRAAWPERGIIVGLLAAWTALAVHHLVDKLYVN
                     NIYVHLGVMIGLLQLFAWPGFEAGSNRGRIDEPLKVDD"
     gene            complement(9243..10187)
                     /locus_tag="PLC06_03660"
     CDS             complement(9243..10187)
                     /locus_tag="PLC06_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO89572.1"
                     /translation="MRVLITGAAGFLGSHLCDRFIAEGHTVVGMDNLITGNMENIAHL
                     YGHDRFSFIKHNVSQYIYVDGPLDAVLHFASPASPNDYLEHPIPTLKVGSLGTHNTLG
                     LAKNKGARFLLASTSEVYGDPQVNPQPESYWGHVNPIGPRGVYDEAKRFAEAITMAYH
                     RYHGLETRIVRIFNTYGPRMRLNDGRAVPNFIYQALTDQPITVYGSGTQTRAYQFYSD
                     LIEGIYRLLLSDEAMPVNIGNPDMEMTVMALAEMTVRLAGSRSEIICIEPTDDRIVDD
                     PQVRRPDITRARTILGWEPRVPVEEGLARTIDFFRQRL"
     gene            10335..11405
                     /gene="fni"
                     /locus_tag="PLC06_03665"
     CDS             10335..11405
                     /gene="fni"
                     /locus_tag="PLC06_03665"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRO89573.1"
                     /translation="MENGPTPSNANEQSADTHDRRKADHIRINLEEDVAFKKVTNGLE
                     SYFFMHQALPELDLAAIDTSIRVLGKTLRTPLLISSMTGGTARAREINRTLAAGAQAA
                     GMAMGLGSMRAAIEDPALEPTYQVRDVAPDILLFANLGAVQLNYGYGLDQCRRSVDMI
                     EADALILHFNPLQEAVQPEGDGNFAGLLHKIEDVCRKLPVPVIAKEVGWGFSREAARQ
                     LSEAGVAAIDVAGAGGTSWSQVEMYRAPTARHARIAGAFIDWGIPTADSIRYCREVAP
                     ELPVFASGGIRDGIDVAKCIALGASLVGLAGEFLRAADRDGVEGVVELAGTIAQELRV
                     AMFCAAAGDLSALAQTPLHKTF"
     gene            11435..12466
                     /locus_tag="PLC06_03670"
     CDS             11435..12466
                     /locus_tag="PLC06_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRO89574.1"
                     /translation="MMNELEKLSAEFLPAIEAEMRSVLGADGSSSHASPFYGMIHYHM
                     GWADENLHPAQINSGKRIRPLLCLLACAAAGGDWQQAVPAAAAIEILHNFSLVHDDIQ
                     DVSPTRRGRPTLWTIWGQPQAINTGDAMFAMAHLAMNRLVERGAAPEIVVQALRRFDE
                     TCVALTQGQYTDMSFEDRKEVDVAEYIAMITGKTAVLIALSAELGALVAGVEDDRVQA
                     FADFGLNLGLAFQVLDDVLGIWGDEVVTGKSVATDITTRKKTLPVLYGLPRNQALREL
                     YDGDATDGEFVRQVVALLDDAGARAYTINEAERYTRQATSSLIVAQPSGSAAEALDIL
                     TGILLRRDF"
     gene            complement(12541..16308)
                     /gene="rpoC"
                     /locus_tag="PLC06_03675"
     CDS             complement(12541..16308)
                     /gene="rpoC"
                     /locus_tag="PLC06_03675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRO89575.1"
                     /translation="MILSGELIVQKGEKVGREHTVQVQEITKERLESIQKTMEDEQKQ
                     ELDELNREIEELQGEIGDAGEGLRERMDQKLVRLRQQAQVNREQLEQLMQMTFLDENK
                     YREYKSKFGNVFRAAMGAEAFHEILRNMDLDKLSRDLWREVRTSRSKQAAKRATKRLQ
                     VVEALRASANRPEWMILTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLK
                     RLLELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRF
                     RRNLLGKRVDYSGRSVIVVGPKLKMGECGLPKTMALELFRPFVISKLVQYGHASNVKG
                     AKRFMERQPPEVWEVLEEVIQGRPILLNRAPTLHRLGIQAFMPKLVEGKAIQLHPLVC
                     AAFNADFDGDQMAVHVPLSDKAVEEAKTLMMASRNLLKPADGQPIVGPSKDMVLGVYY
                     ITMIRDGRKGEGRVFSSLEEVETAYELGVVDIHAKIKVLTKTYWKDDHSRYADGKPRT
                     RIIETSPGRVMFNLALPEQARFVNRMLDKGGVNALINRVHQVIGDVETIEMVDAIKDI
                     GFRFATISGTTVAVADLTIPEQRTEILSTARKRVDEIDRQFRRGLLTEEEQYQRTIEQ
                     WNDAKDQVADAVKQAMDPHGPLAIMALSGAGKGGFGPITQLAGMRGLMADPQGRIIPV
                     PIQSNFRQGLEALEYFISTHGARKGLADTALRTADAGYLTRRLVDVAQDIIIMEEDCG
                     TEKGIYVRRVDNFGKQTLAERLIGRVAAESIVDPETGEILIEKGDYFTTEKADAVDGI
                     KVAEVYVHSPMTCELRLGICRKCYGTDLARGKPVDLGVAVGIVAAQSIGEPGTQLTLR
                     TFHTGGTASAGGDITQGLPRVEELFEARQRPRGEAVMTEIGGIANLRVIDGVRHVFVT
                     DVKLVDDVYEVPEGWTIQVENNQEVTIGQTLAESGDEIIHARHPGRVTLDENGLRVTW
                     ESREERDYEISAGVRLLIGDGQKVQAGDQLTEGSRNPHRILEILGRDAVTQYLLREVQ
                     QVYRPQGQNIHDKHFEVIIRKMLSKVSVTESGDTDMLPGDLVDAPIFQEKNDEVVNEG
                     GQPAQAEPVLLGITKASLNTDSFLSASSFQHTIKVLAGAAIAGKEDRLVGLKENVIIG
                     KLIPAGTGFRVEDDEPIELPEDFFMDPELGGGTRLTSSALIDDDTDVEILAAIAAAAA
                     DASRLTLVSDYEVDEDEESNWDEDEDVWEEEDAEDMEVEGPADDDWEEEEN"
BASE COUNT         3735 a         4874 c         4477 g         3230 t
ORIGIN      
        1 ctcacctgcc cggccagatt gaagatgagg tcctgcccct gcaccagata gcgcaaccca
       61 tgctcatcgc gcatatcggc gatgttgatc cgcaaacggt cttctatgcc atggacatta
      121 tagaagttgc cgccatactc cgggatcagg gaatccagga tcagcacttc cgcccccaga
      181 tcgagcagac gatgggcgag gtttgatccg ataaacccca ggccacccgt gatcagaacg
      241 tgcttgccgc tgaagtaagc gaggtggtca gttggaacgg tcggcataag attggttata
      301 ggaagaattg ggcgcgtcgg ccgccggccg ggggccttcc ttgcgccaca caaggcgata
      361 ccatagcttg gtcaggtcgc gagctacctg cgccagccgg cgaaagcgaa agaactggga
      421 tttgccatag gcgcgatgga aatgatgaac cggcgtctcc ttcagacgga aaccggcatc
      481 ctgcactttt ttcatcaact cgacgcaaat cacgccgcta tcgctggtca gctcaatctg
      541 ctcgaacact tccttgcgca tcagccggaa atcacaatca acatccttca gcttgaggcc
      601 aaaggccagc ttgatcgtgt actggtaaat acggccgatg atgatgcgat ggagtggatc
      661 ctggcgctcg atcttccagc cgttgatgaa atccacctca tcgtcgacta gcgcggccaa
      721 atcctttagt tcccgaggat catactgtgc atcaccgtcg gtgtagaaga tccagtcctt
      781 tgtcgcgcta tagaagccgg tgcgcagcgc gccgccatag ccccgattct gcgggtggtg
      841 gatgatgcgt acttcgtcgg ggtaaatgcg ggccaactcg tcaaggacca tggcggtatg
      901 atcctgacta ccgtcattga caacgatgac ctcataatcg tcagtcagct cacgcaaggt
      961 cagcaatacg gtgatgacca tgctgctaat ggtgccggca tcattatagg ctgggaaaaa
     1021 agcagagatg ctgtagggat tgctcataaa tatcacggca tgggcaggca tcgatcacct
     1081 gcttatgcat catgatttta tcgctttacc gacctttcgc ccaatggcgc gcgtcaccac
     1141 gattacccac atgaccatcg atcttgtccg gctttcccgc tgctcctggc caaacagggc
     1201 aataccataa caccggctgc gatttttagt tgggtcggcc gcgatggtga gttagaattg
     1261 cccacaccac acactactcg gtcgctttct cgccgattta cgtaatctga ggcgcaaatg
     1321 gtcacccgtt atctctcaaa gtcggacttc aaagtagctc gcacctgccc gacaaaatta
     1381 tattatcgaa aaaaaagata tcccacgctg aaagctggtg atgaatattt gtcgatgctg
     1441 gccgatcaag gctacctgat tgaagcactg gcgcgagtat tataccccga tggccgctgg
     1501 atcgggttca ataacgacgt agaagcggcc gcctgggaca cgatgtcggc cctcgccaac
     1561 aattgcaccc tttttgaagc gaccctcatc aatgatgggt tgttggcccg cgttgatatc
     1621 ctgatcaagc gcggcaatgt tttcgagatc atcgagatta agtcgcgcgc cttcgaccgc
     1681 caaaagaatg acgaacgagt tcagcaaggg ctccccaacc tgtttcgtgc cccaagatcg
     1741 gccgacggta tccagagcga atggcggccc tatcttgagg atgtcgcgtt tcaaacaaac
     1801 gtcctgcagc agatattccc tgacgcgacc gtcatccctt atctcctgat gccggacagc
     1861 agccggccgt gccagatcga tggcttgcac cggcaattcg tcttgcgatc acacagcacg
     1921 agtaatgatg gcgacgacat cccctactcc aattacaccg gagagccgca actcatccgt
     1981 cgcaacccat tggtgtgccg ggtcaacgtt caggaagaag taggcatatt gctaccagga
     2041 gtcaggcaac agataaagat atatctggac agcctccggc cggcactcac gcggattaca
     2101 acacccatct caactcattg ccgtagctgc gagtttcacg tcgccgaggg accattaagg
     2161 ggctttcatg agtgctgggg gaagatggcc gatgttaccc ctcacattct cgacctttac
     2221 catgtgagcc aggccggcgg caaaacagcc gacaagctca tatctgaggg caaggcgagc
     2281 ctcttcgatg tgccgatcaa gtctctgtcg cgtcgtgacg gcagcgtggg cgagcacgcg
     2341 cggcggcaac gattgcaaat cgaatgcact cgggacaatc gtgaatggtt cagcgacgag
     2401 cttggagcag tgcttgatac cctgatttac cctcttcgat ttattgactt tgagacgtgt
     2461 gccccgacta ttccccacta tcatgggatg cggccttatg aaacgattgc ctttcaatgg
     2521 agttgccaca ccatcaacgc ccccaacgcc ataccgcaac actacgaatg gctacaatca
     2581 atagacacct ttcccaatga gttgttcgct accacgcttc gcaataacct gggcgacgaa
     2641 ggctcggtac ttatctggtc gaatcatgag atcacgatct tgcgtaaaat tcagcagcaa
     2701 ttggccgaac gagaggctgc ggggtcggat gtgggcgctt ggatcggccg cctgttggaa
     2761 agcgatcgca ttgtcgatct caatcaggtc gcgcagaaac attatctgca tccaagagcg
     2821 ggtgggcgca cttccctgaa agtcatcacg gatgcagttt ggcagtccaa ccccgctatc
     2881 cgccgccggc tgccgcaata cgcggccgaa acggaaagcg agcctaccag cccttaccat
     2941 gtgttgccgc cactctcaat cggcggtcgc caaattgcgg tcgcggaagg aacgggagct
     3001 atcatggcct actacagtat gatggaacgg gcgtcggcca acgccaccct ggaagcagcc
     3061 caatggcgtg acttgttgct gcagtattgc gaactggaca ccatagccat ggtgatggtt
     3121 tggtggcatt ggcgcaaatt aaccgggaag atgagccaat aacactgaaa cggtgacctc
     3181 gctatgtggg tccccgttca ggtgaaaccg atagtccagg cggccgtcgt gtcggccgtt
     3241 aaagccgaga taacatgcat gtcggggcat ggcttgggcg accgcctcag ccaccagacc
     3301 aaactcctgg tcgcgcacaa acaccacatc gatcgcgtgg gtctggggcg catcgagatg
     3361 actcaataaa gaattcaggt ttcgcaagag cgccgtctgc cccatccagg ttcgacaact
     3421 gacggccaca tggttctcac cgttttgaac aactgccaga tcggccggat tcctttccga
     3481 cagggccacg ctgctcgata ggggcgccag gccggattcg agggccagct tcagatggtc
     3541 gcgcagctca gtatcgctct tgcgcgcgta aatggccggc aagcgcgaaa attgggcagt
     3601 cacatccgcg ataatctgta acatgcgccg agcagcagcc gtgtctgacc aggaaggacc
     3661 tgcggagggg cgaccattag cccgcgtggt caccgaactg gccgcatcct tccccttgcc
     3721 gcggcctgta tactcatgga tccaatgacc gaacagggcg ctatccaccc acaggttgtc
     3781 gcccgtggcg gccaggatgc cgtagttttt caatcgttgt acggcgtgat caatctcttg
     3841 ctcgccatat gccagacggg atacaggctt ttcagcagcg acagcaacgg ctacgtctcg
     3901 ctccacatcg ctaaaattgc tccaccattg ttcaaaataa ggccacattt ccgaaaccag
     3961 ccgttgactg atggccgaat gatcaggctt ttttccctga gccttatagg tcagccagat
     4021 agcagccacc gcctggacga agaacggcaa ggtgccggcc tgataacgca cgaaggctac
     4081 atcttcatcg tcggcggaaa tgcccacacg ccgagcggga gctttcacca gatcgtccaa
     4141 ctccgcgggg cttagaccgg acaagtagat gggagtcggg tagaagatat tgtagaaggg
     4201 agatgtgggc ggtaaaccaa cgaatttacc caggcgatag ttgtcccaat atgaggcggt
     4261 cacataagcc aactcgaagt cccagatacc ggccagcgcg cgcaactcgg tcatgaagtc
     4321 cgcgccgaag tcggccgcgc gcaattgatc gaactcatcc atcaagaaaa ccacgcgccg
     4381 ctcgccgaac tcgtagagga gcgactctac gtcataaacg tcgttacctg attgcgaacg
     4441 agctgcgcca gacggcacac gaggcaaatt gctcatcagc ttccggtaca cgcgatcgta
     4501 gaattcagcc ggggtcttgc aggcggacac atcaacatag accatcacat accgttgcgg
     4561 atcaggcaga taggccgcca tgatctcggg atgggttatg tactggaaga acgaggtctt
     4621 gcccgctcgc cgcaaaccca ggacactgac gcattgcatc tgcgtgccgg ccaacgtctc
     4681 aaagaattgc tgcgtttggc tctggcggcc gaagaaatcc gtcggcaatc ggacgggcgg
     4741 cccaacgaca tagggcattc tcatagcttg atctcctcca ccctggtagc ggccaccgcg
     4801 tcaaggccat ggcccgtgcc gttaccatat ccttcccttt ccatccgagc gataacgctt
     4861 tcgacgtctt cagctctgat cgcacttcgc aagtgattgc gattggcctg gctcaccagc
     4921 cggtggcaga ttgactggat gagaaacggg tggccggccg tggcttgcca aatgagttcg
     4981 acggccgtat cctcaacctc aagtacaccc tcaacgggtc ggcgaacaag ctcctccgtt
     5041 tgccgggaat ccagcgggcc aatctcgaac cgagctgtta aatcagcgat tggaccccaa
     5101 aacggctcaa ggagcgcagt agagccgcac agaagaaagg cgatcctggt ccgatgttgg
     5161 atttgcgaac gaatgaaggg caggatttgc gaatccagaa caccgctctc gatcccatcc
     5221 cgaatctgtt caaattcatc tatggccagc aggatgaggc tatcgtctgg taagatacgc
     5281 tccagatagt cgagagagcg atcgaaggta gcatatgccg tttcgtcgga tatctcgcca
     5341 tgagaggcaa cgtcatcgtg attagcgacc tccacacgcc gacaaatatc gcgggccagg
     5401 cggcgcaacc attcatcagt cggccgaccg gcatatcgtt gcaggtcaat gtacaccggg
     5461 atcagatgac gcggccgatc ctctcgcaag gggcgtcctc gctccccttc aaccaactgg
     5521 tacagtgtcg aggtcttgcc gatccgtctc cggccgtaga gcatcaacgc atttggctgg
     5581 cccatgccgg cgaggttctc atcgaaccag tcgaatatct cctcgcgacc cacgtacagc
     5641 gcggtggaca tgccggttag cgggcggccg accacatagg ggttggtgag caatgtgctg
     5701 ttttcgggca gcgaaacatc cataagactc cggtattacc acattccgag cgacaacccg
     5761 gcagcacgca agcagcggcg ccgccatgcc ttccgggcaa agttgctccc aatacagatc
     5821 aaattattgg tactaccgat aaatcacaaa ggcttccgcg ccgagcggcg gcgacccatg
     5881 atgccatctg ttgcaaaata gctcattggt caggggccat tataggtaat tcatacaggg
     5941 gcgacaacaa cgataactaa aaattttcgc cggcgagcag agacgcgtaa agacccgcca
     6001 gttggcgggc agtggcaagc caactgaact gagcgctccg tgccaaccca gccatacgta
     6061 actcatcgcg agcatgttca tcctccaaca atcgaagtat tgtcgtcgtc caggctttct
     6121 catcgtgggg agacaaaagg atcgcgctcg tctctgtggc cacttccggc aggctggacg
     6181 cattggatga gataaccggc gtcccgcacg ccatggcttc cagcaatggc aatccgaacc
     6241 cctcatacag actggggaac accagcaaat cggccgcgga gtacacagcc ggcagatcgg
     6301 cgtcatccac gaacccggcc agtttcaccc tgtctccaag tcccaggcgc tcgatctcgg
     6361 ccggcaagcc ttcatccagc caccccctgc ctccgacgat gacgagcaaa tgcgggtgac
     6421 gttcagccac cggctgaaag gcgcgaacga gcatctcgta atttttgcgc ggctgaaccg
     6481 tgccgacggc cagaacatag ggcctgtcca aaccgtagcg acgacgtact gcactcagcg
     6541 ttgcaccgtc gacgggcctg aaccgctcat tgacgccgct atagagcact gtgatccgct
     6601 cggcggccac accccagagc gattgcagat cacgcgatgt ggagagcgaa tcggccagaa
     6661 tatgcccggc tcgccccaca gaccagggaa cgacccgatt cagataagcg acgagcttcg
     6721 cggggaatgt ctccgggtaa tggacgaaag acagatcgtg aaccgtcagg atcgtcggga
     6781 ttcggccgct gacgggcggc aagacaaaat caggcgaatg aaagaggtcg atcgatcccg
     6841 tgaaggtttg caccgggacg ggtaatcgtg cccgatacca aagccgatag agccaatgtt
     6901 catcgaccgg ggcgggataa tgtcgaacat gcggcgcgac aggcagcgaa tcgggaatcg
     6961 gcggcttggt cggtgggcga gcggagaaga gtttataggt gagttgcggg gcttctatgg
     7021 tcagagcgtg gataagctct cgcgtatatc ggccgattcc cccaccctgc gtgagggcgg
     7081 ccgtcacgtc aaagccaatg accgccccct tccgatcagc cattattcta tgtcgttata
     7141 ggtccaatgc cggttggcaa agaaattcca gaacagaacc accaacacgg ccaccgccag
     7201 cgacaggtta tccgccagcc gggtggtata ggcggccagc gcgccgatat tattgacgag
     7261 cgaccggaac accggcgtca taaagaaaac aataggaata cgaatcagga cgccgaccag
     7321 actcacgata aagaacatcg tgaactggcg gcggcgagag cgggaacggg aatcggggta
     7381 agtccaatat cggttccaga ggaaattgct caatacggcg gccacaaagg aaacggccga
     7441 agcaattgtt ggccccagat gggtgatcaa atagttgctg aacccaaact gggtgagcag
     7501 attatagaat cccgtacccg gcatcaccag cgcattggcg ggatctagca acaaattaaa
     7561 aatgccgaag tcgacgacaa agccgatgcc gcccacaaca agaaatttta tgaaccgttc
     7621 ggtttcccga gggttgaacc caaaccggct ggatacgccg gcgacatatg taatcatcct
     7681 ggacatccct aatcaatcgt caaccttgag aggttcgtcg attcggccgc gattagaccc
     7741 agcttcaaag ccgggccaag cgaacaattg tagcaggcca atcatcacgc ccaaatgaac
     7801 gtagatgtta ttcacgtaaa gtttatccac cagatgatgc actgccaatg ccgtccaggc
     7861 cgctaaaaga ccgacgataa tcccacgttc gggccacgcg gcccgctgcg cataccgaac
     7921 cgactgcccc acaagcacca gccaaaatag tacataggcg agcacccccg gcaggccgac
     7981 ctcggccagc agattcaggt aataattatg ggcatgaccc agggcgtcgg ggaagttgat
     8041 aagatcgtaa tcgccatagg ctgcgccgta atttccaaaa ccgaccccgg tccatagatc
     8101 atcgcgagcc atatcggccg ctgcctgcca atgggccagg cgttcaatga ccgagtagtt
     8161 cgcgtcattg atatcaacgc cgcgcacgtc gcccagcgta aactcttcac ggaagccgct
     8221 caatcgatca atgatagcct gtgatgggcc gaacccggta gccacgccca ggcttagccc
     8281 acctatggtc agggccaaag cggttatcat caccatcagg cccagcgcaa ttcgtcgcgt
     8341 gctgaacaat gccaggaccg cagcaccggc cagaaaacca agccatgcgc cccgactcca
     8401 cgaaaggatg accgccagcg aaccggcgac cgaggcaaga agtgccagcc ccaaccacag
     8461 cccacgaggt gtcagatgat cagccatctg ttccccctgt cgtcgcgacc accaggcaac
     8521 cagcaaaccc aacaataagc ccagcgccgg gaaagcgatg aggttcatat agcccccaaa
     8581 ggggttgggc tgctcaaagg tgccataggc gcgatagaaa cggccgagta ccagaaaatg
     8641 ctccgggcca ttgccccgca gcccaaattg ccagatgcca atcaacgcct gcaggagacc
     8701 gacaagcagc agcatcgcca gtatcaatcg catcaccgcc gcatagccgg acggccgtcg
     8761 ctcaggatcg ctcgccatat ccaggataag ccagacgatc agaccgatct ccagccactt
     8821 gagtagctca acaagcccca aagacaccga ataggcatcg agcagcgaca gcgcagcaat
     8881 caggatgaac agcaaccagg ggatattgaa gcccgtacgc ggaatgagca atctgcgtcg
     8941 ggctaatccc gtcccgatcc aggctgcaaa cgtcaacatc agcgccacct gcccactgtc
     9001 gaataatgat gggccaagga caatcgcttc cagcgcgccg aatggcccca gcaggagcgc
     9061 cagacccaag ccaaacagag ggttaatgag cgcgagaatg aacatcgcgc caatacctac
     9121 taatccagcc gcaatcggca ggggaagccg tgagatcaag acagcgacta ctccggcaag
     9181 gaggtagatt accccttgcg cgcctacgag gtcgccggcg aaacgagtcg gaagctgtct
     9241 aatcaaagtc gttgccgaaa gaaatcgatt gtcctggcca gcccttcttc gaccggcacg
     9301 cgcggctccc aaccgaggat cgtccgcgct cgggtgatgt ccggccggcg cacctgggga
     9361 tcatccacga tgcggtcatc ggtcggctca atgcagatga tctccgaccg gctgccggcc
     9421 agccttacgg tcatctcagc caatgccatg accgtcattt ccatatcggg attgccgata
     9481 ttgacaggca tcgcttcatc agagagcaag aggcgatata tcccctcgat caggtcgcta
     9541 taaaactgat acgcgcgggt ctgtgtgcca ctgccataga cggtgatagg ttggtcggtc
     9601 agtgcctggt aaataaagtt cggcacagcg cggccgtcat tcaggcgcat gcgtggccca
     9661 taggtgttga agatgcgaac gatgcgcgtc tccaatccat gatagcgatg ataagccatg
     9721 gtgatggcct cggcaaagcg cttggcctca tcatagacgc cacgcgggcc aatgggattg
     9781 acgtgtcccc aatagctctc cggctgcggg ttcacctgtg gatcgccata aacttctgac
     9841 gtggaggcca gcaggaaacg cgcacccttg tttttggcta atcccagcgt gttatgcgtc
     9901 cccagggagc cgaccttcaa cgtagggatc gggtgctcca gataatcgtt tgggctggcc
     9961 ggagaggcga aatgcagcac ggcatccagt ggaccatcca catagatata ttggctgaca
    10021 ttatgcttga tgaagctgaa acggtcatgc ccataaaggt gggcgatatt ttccatattg
    10081 ccggtgatca ggttatccat accgactaca gtatgtcctt cggcaataaa acggtcgcac
    10141 agatgcgagc cgagaaagcc ggccgcgccg gtgattaata cgcgcatatg aactccagaa
    10201 cctaagcggg gcgcgcggga atgggtttcg accggcagat caattacggt catcgcgcgt
    10261 cactgattca atccctgatt ttactgcctg tggagtgtac ggtcgaatca tcgtccgtgc
    10321 tataatccac cctcatggaa aatggaccga ccccaagcaa tgccaatgag caaagcgccg
    10381 atacccatga ccggcgtaaa gccgatcata tccgcatcaa tctggaagaa gacgtcgctt
    10441 tcaagaaagt gaccaatggc ctggaatcat acttcttcat gcatcaggcc ctccccgaac
    10501 tcgatctggc ggcgatcgat acgtcaatcc gcgtgctggg aaagacgctc aggactccac
    10561 tactcatttc atccatgact ggaggcacgg cccgggcgcg ggagatcaat cgcacattgg
    10621 ccgccggcgc gcaagcggcg ggcatggcca tgggtctcgg ttcgatgcgc gccgcaatag
    10681 aggatccggc gcttgaaccg acgtatcagg tgcgcgatgt agcgcccgac attttgctct
    10741 tcgccaatct gggcgcggtg cagctcaatt acggctatgg cctggatcaa tgccggcgct
    10801 ccgtagacat gatcgaagcc gacgcactca ttctccactt caacccgctg caagaagccg
    10861 ttcaaccgga aggagacggc aacttcgccg ggctgctcca caaaattgag gatgtttgcc
    10921 ggaaactacc tgtgcccgtg atcgccaagg aagtaggctg gggattctcg cgcgaggccg
    10981 cgcgacaact ctccgaagcc ggagtggccg ccatcgacgt ggccggcgca gggggcactt
    11041 cctggagtca ggtagagatg tatcgcgcac cgactgcgcg ccatgcccga atcgcgggag
    11101 cattcatcga ttggggcatt cccacggccg actcaatccg ttattgccgt gaggtcgcgc
    11161 ctgagttgcc ggtcttcgcc agcggcggca ttcgtgacgg catcgatgtc gccaagtgca
    11221 tcgccctggg agcttcgctg gttggactgg cgggcgagtt tctgagggcg gccgatcgcg
    11281 acggagtgga aggggtcgtg gagctggccg gcacgattgc ccaggagctg cgggtggcca
    11341 tgttctgcgc cgccgcagga gatctctccg cgttggctca aacaccactg cacaagactt
    11401 tttaggaaat agcgcaggct acaaagcaac gattatgatg aacgaacttg agaaattgtc
    11461 ggcagaattt ctgcccgcca ttgaagccga gatgcgcagc gtcctcggcg cagacgggag
    11521 cagttcccac gcgtccccct tctatgggat gatccattac catatgggat gggcggatga
    11581 aaacctgcac ccggcccaga tcaatagcgg taagcggatt cggccgctcc tttgccttct
    11641 cgcctgtgcc gccgccggcg gcgattggca acaggcagtg ccggccgccg cagccatcga
    11701 aatcctgcat aacttttcgc tcgtccatga cgatatccag gacgtcagtc caacccgtcg
    11761 cggccgcccc accctttgga caatctgggg ccagccgcag gcgatcaaca ccggcgatgc
    11821 catgtttgcc atggcccatc tggctatgaa tcgcctggtt gagcgtggcg cggcccctga
    11881 aatcgtcgtc caggccctgc gtcgtttcga tgaaacctgt gtcgctctga ctcaggggca
    11941 atataccgat atgagctttg aggatcggaa agaagtcgac gtagcggagt atatcgccat
    12001 gattaccggc aagacggcgg tgctgatcgc actatcggcc gagttgggcg cgcttgtggc
    12061 cggtgtcgag gacgatcgag tgcaagcatt cgccgatttc ggcctgaacc tcggcctcgc
    12121 ctttcaggta ctggatgacg tcctgggcat ctggggggac gaagtagtca ccggcaaatc
    12181 agtcgccacc gacatcacga cacggaaaaa gacgctcccg gtgctatatg ggctgcctcg
    12241 caatcaggcg ctgcgcgagc tctatgacgg cgatgcaacc gacggggaat tcgtgcgcca
    12301 ggttgtggct cttctggacg atgcaggagc gcgagcttac accattaacg aagccgaacg
    12361 ttacacgaga caggcaacaa gtagccttat tgtcgcacaa ccgtccggct cggcggccga
    12421 agctctggac atattgaccg gcatcctgct gcgtcgcgat ttctaaagcc atgccataaa
    12481 aaagaccgga tggtcaatag ccatccggtc ttcattacaa cttattgccg aagaaaagga
    12541 ttaattttct tcttcttccc aatcgtcatc cgcaggacct tctacttcca tgtcctcggc
    12601 gtcttcctct tcccagacgt cctcatcctc atcccagttg ctctcttcat cttcatcaac
    12661 ttcgtaatcg ctaacgagag tgaggcgaga agcgtcggcc gctgcggcgg cgatggccgc
    12721 cagaatctcg acatccgtat catcatcgat cagggcgctg ctggtcaacc gtgtgccacc
    12781 acccagttcc ggatccatga agaagtcttc gggcaattca atcggctcat catcctctac
    12841 ccggaagccc gtcccggcag ggatcaactt accgatgatg acgttctcct tcagaccaac
    12901 gaggcgatct tccttgccgg cgatggcggc ccccgccaga accttgatgg tatgctggaa
    12961 ggagcttgcc gaaaggaagc tgtcggtatt gagcgacgcc ttggtaatac ccaggaggac
    13021 cggctcggcc tgcgccggct gaccgccttc attgacgact tcgtcgttct tctcctggaa
    13081 gatcggtgca tcgaccaggt cgccgggcag catatcggtg tcgccggatt ccgtcaccga
    13141 aaccttgctc aacatcttgc ggatgatgac ttcgaaatgc ttgtcatgga tgttctggcc
    13201 ctgcggccga tacacttgct gcacctcgcg caagagatac tgtgtcacag catcacgccc
    13261 cagaatctcc aggattcggt gagggttgcg ggaaccctcg gtcaactgat cgccggcctg
    13321 caccttctga ccgtcgccga tcaatagccg aacgccggca gaaatctcgt agtcgcgctc
    13381 ttcgcggctt tcccatgtta cccgcagccc gttctcatcc agggtaaccc gacccggatg
    13441 ccgggcatgg ataatctcat cgccgctttc ggccaacgtc tggccaatgg tgacttcctg
    13501 attattctca acctgaatgg tccatccttc aggcacttca tagacatcgt caaccagctt
    13561 gacatcggtc acaaacacat gacgaacgcc atcgataacg cgcagattgg caataccgcc
    13621 aatctcggtc ataactgcct cgccacgcgg cctttggcgc gcctcgaaga gttcctcgac
    13681 acgagggagg ccctgcgtga tgtcaccacc ggccgaagcc gtaccgccgg tgtggaaggt
    13741 acgcaacgtc aattgcgtgc ccggctcacc aatggactgc gcggcgacga taccgacggc
    13801 gacgcccaaa tccacaggct tgccgcgagc caggtccgtg ccgtagcatt tgcggcagat
    13861 gccgagccgt agctcacagg tcataggcga gtggacataa acctcggcaa ccttgatccc
    13921 gtcaacagca tctgcctttt cagtggtgaa gtaatcgccc ttctcgatca ggatttcgcc
    13981 cgtctccggg tccacgatcg actcggccgc aacacggccg attaagcgct cggccagagt
    14041 ctgcttgccg aagttgtcga cacgacggac atagatgccc ttttccgtgc cgcaatcctc
    14101 ttccatgatg atgatgtcct gcgccacgtc cacaagccgg cgagtcaggt acccggcgtc
    14161 ggccgtgcgc agagccgtat cggccagacc cttgcgcgca ccatgggtgg agatgaagta
    14221 ctccagcgct tccaaccctt gccggaagtt ggactgaatg ggcaccggga taatgcgacc
    14281 ttgcggatca gccatcagac cacgcatgcc ggccaactgg gtgatggggc caaagccacc
    14341 cttacccgcg ccggacagcg ccatgatcgc caacggccca tgcggatcca tcgcctgctt
    14401 cacggcgtcg gccacctgat ccttggcgtc gttccattgc tcgatcgtgc gctgatactg
    14461 ctcctcttcg gtcaacaggc cgcgacggaa ctggcggtca atctcgtcga cgcgcttgcg
    14521 cgcggtggag agaatttcag tccgctgttc cgggatcgtc aggtcggcga cggcaaccgt
    14581 cgtgccggaa atcgtcgcga agcggaaacc gatatcttta atggcatcaa ccatctcgat
    14641 ggtttccaca tcaccgataa cctgatgcac gcgattaata agagcgttaa cgccaccctt
    14701 gtccagcatg cgattgacaa atcgcgcctg ctccggcaac gccagattga acatgacccg
    14761 acccggcgag gtctcaatga tgcgcgtccg cggcttgccg tcggcatagc gcgagtgatc
    14821 atccttccag taggtcttgg ttaacacctt aatcttggca tggatatcaa ccacgcccaa
    14881 ctcgtaagcg gtctccactt cttccaggct gctgaacacg cgcccttcac ccttgcggcc
    14941 gtcgcggatc atggtgatgt aatagacacc caaaaccatg tctttggaag gtccaacgat
    15001 cggctggcca tcggccggtt tcaacagatt gcgcgaggcc atcatcaggg tcttggcctc
    15061 ttcaacggcc ttgtccgaca acggaacatg gacagccatt tggtcgccgt cgaagtcagc
    15121 attgaaagcc gcacaaacca acgggtgaag ctggatcgcc ttgccttcca cgagtttggg
    15181 catgaaggcc tggataccaa gtcggtgtag cgtgggggca cgattcagca ggatcggacg
    15241 tccctgaatc acttcctcca gcacctccca gacctcaggc ggctggcgct ccatgaagcg
    15301 cttggcgccc ttcacgttgc tggcatgacc atattgaacg agcttgctga taacgaatgg
    15361 ccggaacagt tccagtgcca tcgtcttcgg caagccgcac tcgcccatct tcagtttggg
    15421 gccgaccaca atgaccgaac ggccggagta gtcgacgcgc ttgcccaaca ggttgcgacg
    15481 gaagcgtccc ttcttgccct ttagcatatc cgacagggat tttagttcgc gacggccgcg
    15541 acggctcagc gccttaccgc gctggctgtt gtcgatcaga ctatcgacgg cttcctgcaa
    15601 catgcgtttc tcattgcgga cgataacgtc cggcgcgccc agctccaata gcctcttcaa
    15661 gcgattgttg cgattgatca cgcggcgata caggtcattg aggtccgacg tggcgaaacg
    15721 accaccgtcc aactgcacca tcgggcgcag atcgggcggg atcaccggca agacggtcag
    15781 aatcatccac tccggccggt tggcgctggc gcgcaacgcc tcgaccacct gaaggcgctt
    15841 ggtggcccgc ttggcggcct gctttgatcg gctggtgcga acttcacgcc acaaatcacg
    15901 cgacagcttg tccagatcca tgttgcgcag aatctcatga aaagcctcgg cgcccatggc
    15961 cgcacggaag acgttgccga atttgctctt gtattcgcga tacttgttct catcgaggaa
    16021 ggtcatctgc atgagttgct cgagttgctc gcggttaacc tgagcctgct gacggagacg
    16081 gaccagcttc tgatccatgc gctcacgcag cccctcaccg gcatcgccaa tctcaccctg
    16141 caattcctcg atctcgcgat tcagctcgtc cagttcctgc ttttgctcat cttccatcgt
    16201 cttctggatg gattcaagac gttccttggt aatctcctgc acctggacgg tatgctcgcg
    16261 gccgaccttt tcgcccttct ggacgattag ctcgccggac aggatgatat cctcgg
//
LOCUS       DAOMQG010000035        16227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1558_length_16227_cov_9.645375, whole genome shotgun sequence.
ACCESSION   DAOMQG010000035 DAOMQG010000000
VERSION     DAOMQG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 16227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16227
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1558_length_16227_cov_9.645375"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLC06_03680"
     CDS             complement(<1..322)
                     /locus_tag="PLC06_03680"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638126.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HRO89576.1"
                     /translation="MMRYAHIVGWGCYLPERIVTNDELSTMVETSDEWIYQRTGIRTR
                     HIASAEESTATLAFEAAARALKVADLHPSQVDLIIVATSTPEYMFPSTACKVQDYLGA
                     SRAGA"
     gene            423..2438
                     /locus_tag="PLC06_03685"
     CDS             423..2438
                     /locus_tag="PLC06_03685"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HRO89577.1"
                     /translation="MNDPTPPDRPGHGRLLGLIVVLLLVTGSLLLIPALTRFIGSTRW
                     SASRTLGPTRQSADRPTEDAPAALDGKLLPSPSPDDAGDFSAIPANITQLPVPANADE
                     YMAALYDNNPPAHDYFKLAEMLGGLDPGERKAPTDSADVGGRRTFHTHDGARQAELIY
                     QDTLAEYWMETGISLDRSELVAAAQRLREHYYPLLAKNIGAEWDPDADNDPRFTVLLT
                     LGAPDTYELGYFSDEDQYPQALFPSSNEREMVYLNMSQLTPGPLFDGTLVHEVQHLIQ
                     WNLDPNEDKWLNEGLSQIAETMSGLDTVDPRPYLEQTQIRLDGWSKSPPDIYAHYAGS
                     YLYLLYLWEQLGDAAIAELARHPANGPAAVRSVLAGHDPERSLESFTADWAAALYLDG
                     RSTDPRYNIEAFELSPPFFANRARQLPYSESAALEQFGVDLIDLDFTGKATLTFAGDT
                     AVELTGAPPDGLPFWFAPPSNSSDSQLSTEIDLTSAAGATLNFLTWYDLEPSYDFAYF
                     SVSADGGKSWRPLDSVHGILGAYGPAWGGRSAEMMDNDNGWLAESINLDAYAGQKISL
                     RFDVVTDFEGAGHGFAVSAPVIAGIAEQPVWQPDGFVKTGHLLPQRWEVRLIRDGNGG
                     EVLPLRLDDLNRIQTEIELGPEGGALIVIPLTPFVETAADYWLSVTR"
     gene            complement(2446..2655)
                     /locus_tag="PLC06_03690"
     CDS             complement(2446..2655)
                     /locus_tag="PLC06_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89578.1"
                     /translation="MNKFMGFMAGAICGALVGGLAALLITPASGSELIQSAEERWELT
                     LNEARQAMDERRRELETQYRIAKDS"
     gene            complement(2829..4610)
                     /gene="aspS"
                     /locus_tag="PLC06_03695"
     CDS             complement(2829..4610)
                     /gene="aspS"
                     /locus_tag="PLC06_03695"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRO89579.1"
                     /translation="MLKTHSCGELRAEHIGRTVTLAGWVNRRRDMGGVIFIDLRDRAG
                     KTQAVVDSGRTPAGFTTAEGIRSEYVVQVTGVVSRRPAGQENMNLPTGEIEILVDKVV
                     ILNTAKTTPFLIDRDDPVDETTRLRYRYLDLRRERMQRNIILRHNAVKFIRDFLATRD
                     FVEIETPILFKSTPEGARDYLVPSRVHPGKFYALPQSPQQLKQLLMVAGYERYFQIAR
                     CFRDEDLRADRQPEFTQLDMEMSFIERDDLLNLIEELMVGMVQAVSLVPLVSTKFPRL
                     SYAEATERFGTDRPDLRYGMELKDISDIVRDSAFRVFAENVAAGKPVKAICAPGCGTY
                     SRKQIDELEEIARGQGAKALAWLAIQPETGEMRGPIAKFFTVDQLIAITECLDARPGD
                     LILISSDTKAIVNNVLSTLRTELARRLGYTDKKELSFAWVIDFPLFEEGLEDGHFAPS
                     HHMFTAPKPEHISLLDTDPAAVLSQQYDLVCNGFEVAGGSIRIHDQELQAKIMSLIGF
                     SMEQAREQFGHLLEAFEFGAPPHGGIAPGIDRLVALMAGEPNIREVMAFPKTAQATDL
                     MADAPSIVLQRQLDELQLAIVEKPAKA"
     gene            5006..6004
                     /locus_tag="PLC06_03700"
     CDS             5006..6004
                     /locus_tag="PLC06_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRO89580.1"
                     /translation="MAVTHRRLGKHGVLVSNLCLGTMNFGWHTSEEDSFKIMDRALEL
                     GINFFDTADAYGWTVERGHTEEIIGRWLAQGGGRRDAVVLATKVYNLMNRKAGLPEAN
                     SNTRGLSAYKIHKHCEGSLKRLQTDCIDLYQMHHIDRLCPWDEAWQAFGSLVGQGKVI
                     YVGSSNFAGWDIATANQEASKRGMTGLVSEQSIYHLNNRMIELEVIPACRHYGLGLIP
                     WSPLAGGLLGGALEKLEAGRRTSADLEKDVSQNRDKLEKYESLCREIGHPPGEVALAW
                     LLHNPIVTAPIIGPRTLDQLESAVRATEIVLDETVLQKLDEIFPGPGGEAPNAYSW"
     gene            complement(6068..8665)
                     /gene="mutS"
                     /locus_tag="PLC06_03705"
     CDS             complement(6068..8665)
                     /gene="mutS"
                     /locus_tag="PLC06_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRO89581.1"
                     /translation="MTDTVTPSRRQYLEIRAQHPDAIVFFRLGDFYETFDDDAAVVAR
                     ELDLVLTSRPQGKNERVPMAGVPHHAAENYIARLIAKGYKVALCEQMGSDTVNGLMPR
                     EVVRVFTAGTVVEPGMLEAGRNNYIVAVVLDDGRAGLAYADITTGEFATTQLAGHRDL
                     IEELARLSPVELLLPEEMDGGFAAVKTISRLPAHRFELGNARQTLLRHFEVSALDAFG
                     CEQKPLATRAAGALLYYLQQTQRGSVGQMQRLTTYTTDGYMALSEATRRNLELTEPIA
                     ARPGGEREGTLLATLNKTVTPMGARLLRQRITQPLLSIEALNERLDQVQAFLENAMLR
                     AELRAALKGMPDLERLTNRVLGGRATPRDLEQIAVALGAVERVKRLAGSAAPLAAAAG
                     DLDPCHEVAGTIGRALREDAPTNLNKSGFFRSGFSAELDGVISTSAHARDWVAELEPR
                     ERERTGILSLKVGFNKVFGYYLEITHANTKLVPSDYIRKQTLTNAERYITPELKEYET
                     LILNAEERILQIERRLFAELSQYVAGYGVQLLATAAALAELDAAAALAEVAALNDYVR
                     PQLTDGIELHIENGRHPVVERALSRGLVPGMARGERFVPNDADFDDGSVIQIITGPNM
                     SGKSTYLRQVALIALMAQIGSYVPASSARIGLVDRIFTRIGAHDELHAGRSTFMVEMV
                     ETAEILHHATHRSLLILDEIGRGTSTYDGLSLAWAIVEFLHNHPRLNPRTLFATHYHE
                     LTGLADFLPHVVNYNVGVSEEGGEVTFLHRIVPGGADRSYGIHVAQLAGLPRDVILRA
                     NEILLDLERHAPTAAVEPSRLSSGQQIALFPESSPFLDELTQLDVNALTPLQAINKLY
                     EWKQRYTKT"
     gene            complement(8684..9952)
                     /locus_tag="PLC06_03710"
     CDS             complement(8684..9952)
                     /locus_tag="PLC06_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRO89582.1"
                     /translation="MTTLKPESIVSLATGQRSLGDRAVTYPLFPPLVEGCPRTSTAEL
                     QYPLVIDYDYKKVPADFFGRDPYAGIWRWGELLPPLAPGLSMGEGGTPLVAAPHLANW
                     AGIDSEVYIKDESRNPTGSHKDRLNLCTVSSAVLTGAPGITVASSGNHGAAAAAYAAR
                     AGLPCVLFITEGTEPHLVRMVSSYGAAVVPLPRPLRRVLMRELVYRAGYMPVSSITET
                     HTGHPFGPEGYKTIAYELYRQLGSRFPAAVFVPTAYAELLYGVWLGFKDMQRVGGIGP
                     LPQMIACEPAAGAPLRAALAAGRPVVKVDEAPTAAYSIVVGSNSYRGVLAVQESEGDA
                     LALTDAEIATAQTALGREGLWAEFSGAASVAGLRQAAAAGGRFDGPVVCLMTSMGFKD
                     QGLPAPELPPVSPTWEAVERTLRDVYGLTV"
     gene            complement(9949..10482)
                     /locus_tag="PLC06_03715"
     CDS             complement(9949..10482)
                     /locus_tag="PLC06_03715"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HRO89583.1"
                     /translation="MNPFHWAFDKFYPIIRYVYERVQGHRWFDRITPNLWLGGAPTYD
                     RDYQFLLDNNINAILDMRAEREGDREFYNHHDINYKHLKVLDATVPSDEYMTEGADWI
                     DAQIVEGRSVLVHCAKGRGRSATLVAAYLMRHKGLSYDEAHTMMKEARPLTKLESRHR
                     RRLEAWNEQSKAAEDIR"
     gene            complement(10599..11588)
                     /locus_tag="PLC06_03720"
     CDS             complement(10599..11588)
                     /locus_tag="PLC06_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO89584.1"
                     /translation="MKVLFIGGTGIISSACSALAVEQGMDVYLLNRGQTSHRPIPEGA
                     KVLYGNIRDRVSTANALDDHTFDVVIDWVAYTPDHIATDLDIFSGRVGQFIFISSASA
                     YQKPPARLPITESTPLDNPFWQYSRDKIACEAVLLDACQRNNFPVTIVRPSHTYDRTL
                     LPFHGGYTIIDRIRRGKPLLIHGDGTSLWTLTHHRDFARAFLGLLGESRAIGEAYHIT
                     SDEWLTWNEIARLLGNAVGVEAQLAHVPSETVARFDPGWGDSLLGDKSHCSIFDNSKI
                     RGIVPGFAAEIPFSRGAREIVGWYDANPDRQLVDNTFDQLMDRIIAAQAAVLP"
     gene            12110..>14508
                     /gene="acnA"
                     /locus_tag="PLC06_03725"
     CDS             12110..>14508
                     /gene="acnA"
                     /locus_tag="PLC06_03725"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRO89585.1"
                     /translation="MTKRLFDKFGARAKLPGTDYHYYQLNKLSKFADISRLPFSIKIL
                     LEAVLRTADGYEVMPEDVERLAGWSPKMTAPFEIPFKPGRVILQDFTGVPAVVDLAAM
                     RAAMARLGGDPERINPIVPVDLVIDHSVQVDQFGSAFALFFNAEREFERNRERYEFLK
                     WGQSAFQNFRVVPPATGIVHQVNLEYLGKVVMTKPEGDGLVAFPDSLVGTDSHTTMIN
                     GLGVLGWGVGGIEAEAVMLGQPIYMLTPEVVGVRLTGSLPEGTTATDLVLRITEMLRK
                     KGVVGKFVEYFGPGLSNMTLADRATIANMCPEYGATVGFFPVDNETLNYMRNTGRSAE
                     LIDLVERYTKEQGLFRTDSTPDPDFTDVVELDLSSIEPSLAGPKLPQDRIPLKNMRES
                     FHQSLVAPVGPRGFGLGEKDLDRTAEISNGHTSEIGHGAVVIAAITSCTNTSNPSVMV
                     AAGLLAKKAVEAGLTVPPYVKTSLAPGSRVVEDYLKRAGLLPYLEQLGFYIVGFGCTT
                     CIGNSGPLPEAVSQAIHESDLVVSSVLSGNRNFEGRVHPDTRSNFLASPPLVIAFALA
                     GRADLDMYNEPLGNGRDGQPVYLRDIWPTNKEISRVMTESLTPEMFEHEYGNVFTGNE
                     TWNLIPTGDGELYTWDETSTYIQEPPFFAGLSHEVNAITNIEGARVLVKVGDSITTDH
                     ISPAGSISRTSPAAHYLTNHEVEPRDFNSYGSRRGNDRVMTRGTFANIRLRNQMAPGT
                     EGGMTTYLPTGEVMTIFDASLRYKETGTPLVALAGREYGTGSSRDWAAKGPLLLGIRL
                     V"
     assembly_gap    14509..14608
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14609..14964
                     /locus_tag="PLC06_03730"
     CDS             <14609..14964
                     /locus_tag="PLC06_03730"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588057.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRO89586.1"
                     /translation="SRDWAAKGPLLLGIRLVIAESFERIHRSNLVGMGILPLQYKVGE
                     NAQTLGLDGAETFSTLNLDDDLKPGQTVRVRAEHPDGHVVEFETICRIDTPVEVDYYR
                     NGGILHTVLRQFLAN"
     gene            14968..16149
                     /locus_tag="PLC06_03735"
     CDS             14968..16149
                     /locus_tag="PLC06_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRO89587.1"
                     /translation="MRFPTIRPVQGRAKLRPALLLAIIALVLLPAACRQQPVVPESNN
                     GTAMHRANPARTGVYQTKGPTKYESIKWQFEADDWVFGAPAVANGTVYFTSYDGRVYA
                     ADAETGAEKWRVDTGTTIIASPAVSNGLVYVANMAGSLLALDAGSGQERWRVETETGH
                     SGSPAVVDGVVYFGGESGLLVALDADTGEERWRFQIPGTVIAFSTAVADGLVFVPVSN
                     GELHVLDAATGEEQWKFDPNSSDNIYSPTADPVVHNGSVYFMVSDINLTGVLFAIDLN
                     THQLRWEHKTSTENFSAPAILDDILVWGSLEGILYGVSATDGEVLWTFPTDDIIFSAP
                     AIANGVVYFGSHDKNLYAVDYRTGKQLWQFRAESGVSSPAIDNGIVYVGTLDGAFYAL
                     Q"
BASE COUNT         3328 a         4922 c         4679 g         3198 t
ORIGIN      
        1 acgcccccgc ccggcttgct cccagatagt cctgcacctt gcaggccgtc gaagggaaca
       61 tgtactccgg tgtggatgtg gcgacgatga tgagatcaac ctgcgacggg tgcagatcgg
      121 ccactttcag ggcacgggcg gccgcctcga acgccagcgt cgccgtcgac tcttcggccg
      181 aggcgatgtg gcgggtgcga ataccggtgc gctggtagat ccattcatcg ctggtttcta
      241 ccatcgttga gagttcgtcg ttggtcacga ttcgttcggg gagatagcaa ccccaaccaa
      301 cgatgtgggc atatcgcatc atatgttttg accgttgaac atcttctaca ttggtataca
      361 acactacccc attggaacag ttgcgccggg gcttgccaaa tagatacaat cgctcattat
      421 cgatgaatga tcccactcct cccgatcgtc ccggccatgg cagacttctt ggcctgatcg
      481 ttgtcctgct tctggtgacg ggcagccttc tgctcatacc ggcgctcacc cggttcatcg
      541 gctcgacgcg atggtcggca agtcgaacgc ttggccccac gcggcaatcg gccgatcgcc
      601 ccaccgaaga cgcaccggcc gctcttgacg gcaagctact gccctcgcca tccccggacg
      661 acgccggtga tttctccgcg attcctgcaa acattaccca gctgcctgtc cccgccaatg
      721 cagatgaata catggcagcc ctctacgata acaacccgcc ggcccatgac tacttcaaac
      781 tcgccgagat gctgggcggg cttgatccgg gcgagcgaaa agcgccgacc gatagcgccg
      841 acgtcggcgg ccgacggact tttcacaccc acgacggcgc tcgacaggcg gaactaattt
      901 atcaggatac gctggctgaa tattggatgg aaacgggcat ttctctcgat cggagcgagt
      961 tggtcgccgc cgcccaacgc ttgcgggagc attactaccc cctgttagcc aaaaatatcg
     1021 gcgccgaatg ggatcccgac gccgacaacg acccacggtt cacagtcttg ctcacattag
     1081 gcgcgcccga tacctatgag ctgggctatt tttccgatga ggatcaatat ccgcaagcgc
     1141 tcttcccgtc gtccaacgag cgcgagatgg tctatctgaa catgtcgcaa ctaacaccgg
     1201 ggccgctctt cgacggcacg ctggtgcatg aagtgcaaca tctcattcaa tggaacctcg
     1261 acccgaacga ggacaagtgg ctcaacgaag gtttgtcgca gatcgccgag acgatgtccg
     1321 gcctggacac ggttgacccg cggccgtatt tggaacaaac gcaaattcgg cttgatggct
     1381 ggtcaaagtc gccgccggat atctacgccc actacgcggg cagctatctc tatctgcttt
     1441 atctgtggga acagttgggc gacgccgcca tcgccgaact ggcccgccat ccggccaacg
     1501 ggccggcggc cgttcgcagt gtgcttgccg gccatgaccc ggagcgctca cttgaatcct
     1561 ttaccgccga ttgggctgcg gcgctctatc ttgacggtcg atcaaccgac ccccgctaca
     1621 atatcgaggc cttcgagctg tccccgcctt tcttcgccaa tcgtgcccgg caactgccct
     1681 attcggaaag cgcagctctc gagcagttcg gcgtcgatct gatcgacctc gacttcaccg
     1741 gcaaagccac cctcaccttc gccggcgaca cggcagttga actgaccggc gcgccgccgg
     1801 acgggttgcc cttctggttc gccccgccct caaacagcag cgactcccaa ctgtccaccg
     1861 agatcgatct gacgagtgcc gccggcgcga cgctgaactt cctcacctgg tacgatctgg
     1921 aaccgtccta cgatttcgcc tacttctcgg tgtcggccga tggcggaaaa agctggcggc
     1981 cgctcgattc ggtgcatgga atcctcggtg catacggccc ggcctggggc ggccgcagcg
     2041 cggagatgat ggacaacgac aacggctggc tcgccgaatc gatcaatctc gacgcctacg
     2101 ccgggcaaaa gatatccttg cgcttcgacg tcgtcaccga ttttgagggg gcggggcacg
     2161 ggtttgccgt cagcgcaccg gtgatcgccg gcatcgccga gcagccggtc tggcaaccgg
     2221 acggattcgt caaaacgggg cacctcctgc cccaaaggtg ggaagtaaga ctcatccgcg
     2281 acggcaacgg aggcgaagtg ctccccctgc gcctggatga tctgaatcga attcagaccg
     2341 agatcgaact tggccccgaa ggaggggcgc taatcgtgat accgctgacc ccgtttgtgg
     2401 aaacggccgc ggattactgg ctatcggtca cccgataagg tcgatctagg aatccttggc
     2461 gatccggtac tgcgtttcca gttcgcggcg acgctcatcc atcgcctggc gcgcctcgtt
     2521 caacgtcaac tcccaacgct cctcggcact ctggatcagt tccgaccccg acgccggggt
     2581 gatgagcaag gcggcgaggc caccgactaa tgcgccgcag atcgcaccgg ccataaatcc
     2641 cataaatttg ttcatttctt ccctctacaa gccgagatca ctatcggcca ccatagctga
     2701 tagacgaaat tatacaactt ttccccatca tccggccaag cggcgttcga aagaatcaaa
     2761 aggcgaggcc aacaggattg ccgttgacct cgccttgtct gatcaaaccc aaccacgttt
     2821 caggtagctt acgcctttgc gggtttctcc acgatcgcca actgcaactc atccaactgg
     2881 cgttgcagca cgatcgacgg cgcgtcggcc atcagatcgg tcgcctgagc tgtcttgggg
     2941 aaagccatca cctcgcggat attcggctcc ccggccatca gcgccaccag ccgatcgatg
     3001 ccgggcgcaa tgccgccatg cggcggcgcg ccaaactcga acgcttccag caaatggccg
     3061 aattgctcgc gagcctgctc cattgagaag ccgatcaggg acatgatctt ggcctgcaac
     3121 tcctgatcgt ggattcggat gctgccgccc gccacctcga agccattaca caccaggtcg
     3181 tattgctggc tgaggacggc cgccggatcg gtatcaagca aggagatatg ctccggcttg
     3241 ggcgcggtga acatatggtg gctgggcgca aaatgaccat cttcaagccc ttcctcaaag
     3301 agcgggaagt cgatcaccca ggcgaaggac agctccttct tgtccgtgta acccaaacgg
     3361 cgggccagct ccgttcgcag cgtgctcaac acgttgttga cgatggcctt cgtgtcgctg
     3421 gagatcagaa tcaggtcgcc cggcctggca tcgaggcact ccgtaatggc gatcaactga
     3481 tcaaccgtga agaacttggc gatcggcccg cgcatctcgc ccgtttcggg ctgaatggcc
     3541 agccaggcca gcgccttggc cccctgccct ctggcgatct cttccagctc atcgatctgc
     3601 ttgcggctat atgtgccgca gccgggagcg cagatggcct tcaccggctt gcccgcagcc
     3661 acattctcgg caaagacgcg gaaggcgctg tcgcgaacga tatcgctgat atccttgagt
     3721 tccatgccgt agcgcagatc gggccggtcg gtgccaaacc gctcggtcgc ttcggcatag
     3781 cttagccggg ggaacttcgt ggaaaccaac ggcaccagac tgaccgcctg aaccatgccc
     3841 accatcaact cctcgatcag gttgagcaga tcgtcccgct cgatgaaact catctccatg
     3901 tcgagctgag tgaattccgg ctggcgatcc gccctcaggt cttcgtcgcg gaagcatcgg
     3961 gcgatctgga aatagcgctc gtagccggcc accatgagca attgcttcaa ctgctgcggg
     4021 ctttgcggca aggcatagaa tttgcccgga tggacgcggc tggggacgag atagtcgcgc
     4081 gcgccttcag gcgtggactt gaacaggatt ggtgtttcga tctcgacaaa gtcccgtgtc
     4141 gcaagaaaat cacggatgaa cttcacggcg ttgtggcgca ggatgatatt gcgctgcatc
     4201 cgctcgcgtc gcaggtccag gtagcgatag cgcaggcggg tcgtctcatc caccggatca
     4261 tcgcggtcaa taagaaacgg cgtggtcttg gccgtgttca ggatgaccac cttatccacc
     4321 agaatttcga tctcgccggt gggtaaattc atgttttcct ggccggcagg ccgccgcgag
     4381 acgacgccgg tcacctggac gacgtattca ctgcggatgc cctcggccgt cgtgaagccg
     4441 gcgggcgtgc ggcctgaatc gacgaccgcc tgcgtctttc ccgcgcggtc gcgcaagtcg
     4501 atgaagataa cgccgcccat gtcgcgccgg cgattgaccc aaccggccaa cgtgacagtc
     4561 cggcctatgt gttcggcgcg aagttcgccg caactatgcg tcttcagcat atgattcctt
     4621 cctgaatgga tagtaatatt tctcctccgt ccttcgagag acgtggagcg atcggagatt
     4681 ataccacgcg cggccatttg ggtcagcgct ttgtatggcg ctttcgcatc tgatgcaaag
     4741 caggatagtt tatcaatccg ggtctgtcta gagctattaa ggcctacaac ctgttcagta
     4801 atttcttcgc tgcctcccaa ccctcctgaa ctgcaccgcg aaacgctgac tgtgccgtct
     4861 tccactctgc atgcgtatca gaagtggaaa caaccagcta ccggatccag cgacacaatc
     4921 attggcagga gatagtacct gatggaaaat caggcagagg cattcttctg gttgatcaga
     4981 caacaaaacg ataaggagtt caataatggc cgtaacacac agacgattag gtaaacatgg
     5041 tgtgttggtt agcaaccttt gtctggggac catgaatttt ggctggcaca cgagcgagga
     5101 agatagcttc aagattatgg accgcgccct tgaactaggg atcaacttct tcgacacggc
     5161 tgatgcttat ggctggacag tagaacgcgg ccataccgag gaaattattg gccgctggtt
     5221 ggcgcaaggc ggggggcggc gggacgcagt agtcttagca acaaaagtct ataatttaat
     5281 gaatcgaaaa gccggcttgc cggaagccaa cagcaacacg cgcggcttgt cagcctacaa
     5341 aattcacaag cattgtgagg gtagcctcaa gcgcctgcaa acagactgta tagacttgta
     5401 tcaaatgcac cacattgacc gtctatgccc gtgggacgag gcctggcagg cgtttggcag
     5461 tcttgtaggt caagggaagg tcatttatgt cggttccagc aattttgccg gttgggatat
     5521 agccaccgcc aatcaggagg catccaaacg aggaatgacc ggattggtga gtgaacaaag
     5581 catttatcat ctcaataacc gcatgatcga actggaggta atcccggcct gccgtcatta
     5641 tggcttggga ctcatcccct ggagtcccct ggcgggcggt ttgttaggcg gcgctttgga
     5701 aaaactggaa gccggccgcc gcacaagcgc cgatttagaa aaagacgtta gccaaaatcg
     5761 ggacaagctg gaaaagtacg agagcctttg tcgcgaaata gggcatccac cgggtgaagt
     5821 ggcgttggcc tggttactgc acaatccgat tgtgaccgct ccaatcattg ggccgcgcac
     5881 cctggatcag ttggaaagcg cggtccgagc cactgagatt gtgctggatg aaacggtctt
     5941 gcaaaaactg gatgagattt tccccggccc gggcggcgaa gccccaaatg cctactcctg
     6001 gtaacatccg aggtaaattc caggtcaatt atagttaaga cttgctatcg tcgggatgct
     6061 taaggggtca ggtctttgtg tagcgctgct tccactcata cagcttgttg atcgcctgca
     6121 gcggtgtcag ggcgttgaca tcgagctgcg tcagttcatc gaggaacggg ctggactccg
     6181 ggaagagagc gatttgttga cccgacgaca ggcgactggg ttcaacggcg gccgtcggcg
     6241 cgtggcgctc cagatcgagc aggatctcgt tggcccgcag gatcacgtcg cgcggcagtc
     6301 ccgccagttg agccacatga ataccatagc tgcggtccgc gccgccgggc acaatccggt
     6361 gcaggaacgt cacctcgccg ccttcctcgg atacgcctac gttgtaattc accacgtggg
     6421 gcaggaagtc ggccagcccg gtcaactcgt ggtaatgggt ggcgaacagc gtgcgcggat
     6481 tcaggcgcgg atgattatgg agaaactcga cgatggccca ggccagtgac aggccgtcat
     6541 aggtgctggt gccgcggccg atctcgtcca ggatgagcag cgagcgatgg gtggcgtgat
     6601 gaaggatttc ggccgtctcc accatctcga ccatgaacgt cgagcgcccg gcgtgcagtt
     6661 catcatgggc gccaatgcgg gtgaagatgc gatccaccag accgatccgg gccgacgatg
     6721 cgggcacata gctgccaatc tgggccatca gggcgatcag cgccacctgc cgcaagtagg
     6781 tcgatttgcc gctcatgttc ggcccggtga tgatctgaat gactgagccg tcgtcaaaat
     6841 cagcgtcgtt gggcacgaag cgctcgccgc gagccatacc cggcaccaat ccccggctta
     6901 acgcccgctc gacaaccgga tggcggccgt tttcgatgtg gagctcgata ccgtcggtca
     6961 attgcggacg aacgtagtcg ttgagcgcgg ccacctcggc cagcgccgcg gccgcgtcca
     7021 gctcggccaa cgccgcggcc gtcgccagca gttgtacgcc atagccggcc acgtattggc
     7081 tcaactccgc gaacaggcga cgctcgatct gcaggatacg ctcttcggcg ttcaggatga
     7141 gcgtctcgta ctccttcagt tcaggtgtga tatatcgctc ggcgttggtc aacgtctgct
     7201 tgcgaatgta gtcggacggg acgagcttcg tattggcgtg ggtgatttcc aggtagtagc
     7261 cgaacacttt gttaaagccc actttcagcg acaggatgcc ggtccgctcg cgctcccgtg
     7321 gctccagctc ggccacccag tcgcgagcgt gagccgaggt tgagataacg ccatccagct
     7381 cggccgagaa acccgaccgg aagaagccgc ttttgttcag attagtgggc gcatcttcgc
     7441 gcaaggctcg gccgatcgtc ccggcaacct catgacacgg gtcaaggtcg cctgcagccg
     7501 cggcaagagg cgctgcactg ccggccagcc gcttcacgcg ctcaaccgct cccaatgcga
     7561 ctgcgatctg ctccagatcg cgcggcgtcg cccgccctcc caacacgcgg ttggtcaggc
     7621 gctccaaatc gggcatgccc ttaagagcgg cgcgcaattc cgcgcgaagc atggcatttt
     7681 cgagaaatgc ctgcacctga tcaagtcgct cattgagcgc ctcgatactc agtaacggtt
     7741 gggtgattcg ctggcgcagc aaacgggcgc ccatcggggt caccgtcttg ttgagcgtgg
     7801 ccagcagcgt tccctcgcgc tcgccgcccg ggcgagcagc gataggttcg gtcaactcca
     7861 gattacggcg agtggcttcg ctcagcgcca tgtagccgtc ggtggtatag gtcgtcagcc
     7921 gctgcatctg gcccacgctg ccgcgctggg tctgttgcag atagtagagc agcgctccgg
     7981 cagcgcgagt cgccagcggc ttctgctcgc aaccgaacgc gtcgagcgcg gaaacctcaa
     8041 aatgacgcag caaggtttgc cgggcgttgc ccagttcaaa gcgatgggcg ggcagccggc
     8101 tgatggtctt caccgcggca aaaccgccat ccatctcctc cggcagaaga agctcgaccg
     8161 gcgaaaggcg agccagttcc tcaatcagat cgcgatggcc ggccagttgg gtggtggcaa
     8221 attcgcccgt ggtgatgtcg gcataggcca gcccggcgcg gccgtcatcc aataccacag
     8281 cgacgatgta gttgttgcgc cctgcctcca gcatccccgg ctcaacgact gtgccggcgg
     8341 tgaaaacgcg gacgacctcg cgcggcatga ggccattcac cgtgtcgctg cccatctgct
     8401 cgcacagggc gaccttgtat cctttggcga tgaggcgggc aatgtagttc tcggccgcgt
     8461 gatgaggcac gccggccatc ggcacacgct cgtttttgcc ttgcggccgg ctggtcagga
     8521 caagatcaag ctcgcgggct acgacggccg cgtcatcgtc gaacgtctca taaaaatctc
     8581 ccaggcggaa gaagacgatg gcgtcgggat gttgcgcccg aatttcgagg tattggcggc
     8641 ggctgggggt gaccgtgtca gtcatgggca aatcaacgcg aacctagacc gtcaacccgt
     8701 aaacgtcgcg cagcgttcgt tccaccgctt cccaggtagg cgaaacgggc ggcagttcgg
     8761 gcgcgggaag cccttgatcc ttgaacccca tggaagtcat caggcagacc accgggccgt
     8821 cgaagcgccc gcccgcggcg gccgcctgcc gcaatccggc gacgctcgcc gcgcccgaaa
     8881 actccgccca caggccttca cggcccaaag ccgtttgcgc cgtggctatc tctgcatcgg
     8941 tcagggcgag ggcatcgcct tcgctttcct gcacggccag aacgccgcga tagctgttgg
     9001 aaccgacgac gatgctgtag gcggccgtcg gtgcttcatc caccttgacg acgggccgtc
     9061 cggcagccag cgcggctcgt agtggcgcgc ctgccgccgg ctcgcaggcg atcatctgcg
     9121 gcaacggccc aataccgccc acgcgctgca tatccttgaa gcccaaccag acgccataaa
     9181 gcagttcggc ataggccgtg ggcacgaaga cggccgccgg aaatcgactc cccaactgcc
     9241 ggtagagttc ataggcgatg gtcttgtatc cttcggggcc gaacggatgg ccggtgtggg
     9301 tctcggtaat gctgctgacc ggcatatagc ctgcccgata caccaactcg cgcatgagca
     9361 ctcgccgcaa tgggcggggc agcggcacga cggccgcgcc ataggacgac accatgcgga
     9421 cgagatgcgg ctccgttccc tcggtgataa ataacacgca aggcaacccg gcgcgggcag
     9481 cgtaagcggc ggccgcggcg ccatgattcc ccgatgatgc cacagtgatg cccggcgcgc
     9541 cggtgagaac cgccgaactg accgtgcaca gattcagccg atccttgtgg ctgccggtcg
     9601 ggttgcggct ttcatccttt atgtaaacct cagaatctat tccagcccag ttcgcaagat
     9661 ggggcgcagc caccagcggc gtgccgcctt cgcccatcga caggcccgga gcgagcgggg
     9721 gcagcagctc gccccagcgc caaatacccg cgtaggggtc ccgaccgaag aaatcagccg
     9781 gcactttttt ataatcgtag tcgatcacca atgggtattg cagctcggcc gtgctcgtac
     9841 gcggacaacc ttccacgagc ggcggaaaca gcggataagt cacggccctg tcgcccaagg
     9901 aacgctgacc ggtggccagg gaaacaatcg attcaggctt cagggtagtc atcggatgtc
     9961 ttcggcagct ttggattgct cattccaggc ttcgagccgg cggcggtggc gactctccag
    10021 cttggtcagc ggccgggcct ctttcatcat ggtgtgggct tcatcgtaag acaagccttt
    10081 gtgccgcatc aaataagccg ccaccagcgt cgccgagcgg ccgcgtccct tggcacaatg
    10141 aaccagaacc gatcgtccct caacgatctg cgcgtcgatc cagtcggcgc cctcggtcat
    10201 atattcatcg cttggaacgg tcgcatcgag cactttcaga tgcttgtaat tgatgtcgtg
    10261 gtgattgtag aactcgcgat ccccttcacg ctcggcgcgc atgtcgagga tggcgttgat
    10321 gttgttgtcc agcaagaact ggtaatcccg gtcgtaggtt ggcgcgccgc caagccacaa
    10381 attaggtgtg atccggtcga accagcggtg accctgcaca cgctcgtaga catagcgaat
    10441 gatgggatag aacttgtcga aagcccaatg gaatggattc atcgcccgta aggataaccc
    10501 tggcggcgat ggttggcaat gggaatgaaa ggatgcgtca gcgcgatttc gatgcttatg
    10561 tcaagatagg atgttgtgcg ggccttcagg cctgagcctc atggcaggac tgccgcttgc
    10621 gcagcgatga tgcggtccat caactggtca aaagtattgt caaccaattg tctgtcggga
    10681 ttggcgtcgt accagcccac gatctcgcgc gcgccccggc tgaacggtat ctcggccgca
    10741 aatccgggca cgatgccacg aatcttgctg ttgtcaaaga tgctgcaatg ggatttgtca
    10801 cccaggaggc tgtcgcccca tccggggtcg aaccgggcaa ctgtctccga gggcacatga
    10861 gccaattgag cttcaacgcc aacagcattg cccaacaagc gcgcaatctc gttccaggtg
    10921 agccattcat cactggtgat atggtatgcc tcgcctatag ctcgcgactc ccccagcagc
    10981 cccaaaaatg cccgggcgaa gtcgcgatgg tgagtcagcg tccataacga cgtgccatcg
    11041 ccatggatga gcaagggctt gccgcggcga atccgatcga taatggtgta acccccatga
    11101 aagggcagca gtgtccgatc gtaggtatgc gacggccgca cgatagtcac cggaaagttg
    11161 ttccgttgac aggcatcgag caataccgct tcgcaggcga tcttgtcgcg cgaatactgc
    11221 cagaacgggt tgtccagcgg cgtgctctcg gtgataggca accgggccgg tggtttctga
    11281 taggccgaag cggaactgat gaagataaac tggccgacgc ggccgctaaa aatgtccagg
    11341 tcggtggcga tgtgatcggg tgtgtaggcc acccaatcaa tgacgacatc gaacgtatga
    11401 tcgtccaacg cgttggcggt tgatactctg tcgcgaatat tcccgtatag cactttcgcg
    11461 ccctcaggga tgggccgatg actcgtctgt ccccggttga gcaaatacac gtccataccc
    11521 tgctccacgg ccaaagccga acaggccgaa ctgataatgc cggtgccgcc gataaacaag
    11581 accttcacaa tgacctactc cttgctgata tacccgattg tcgaaaacgg attactgtca
    11641 gcgctctcgc cgatctatca attatcaatc ccgccgctcc acatatacgg ctattccatc
    11701 ttttgggcgc agtgtaacca gagcatgccc ctcaaccggg tcagacgatg cccgccgcag
    11761 ccggaagcga ccggcaatgg cgggaatgag gatgattcaa tggggcaggt gttgtttcgt
    11821 tgatcatcga gttcatgccg gccaccggca ttctaaaata cccatctgct tccccatata
    11881 gtatagccaa ttcggccgtg ataacggcaa agcgtcaagc cgcctcaccg ttcaatgatc
    11941 taaaacgccg gcatggctgc catgtttcca gaccaattaa atcagctata atcactttac
    12001 atctggatta aaatacgcat tccaaaggcc aaaagatgta aacttccatg aaggcctgtg
    12061 tcgcaagtcg aacaggattg aacagaggcc tttatagttg aggagaacca tgacgaaacg
    12121 attattcgac aagtttggcg cgcgcgccaa actccccgga accgattacc actactacca
    12181 actcaataag ttgtccaaat tcgccgacat cagccggctg ccgttttcca tcaagatatt
    12241 gctggaggcg gtgttgcgca cggccgatgg gtacgaagtt atgcctgagg atgtcgagcg
    12301 gttggccggc tggtcgccga aaatgaccgc gccatttgaa attcccttca aacctggtcg
    12361 cgtcatcctg caggacttca ccggcgtgcc ggcagtcgtc gatctggcgg ccatgcgcgc
    12421 ggcgatggcc cgattgggcg gagacccgga gcgcatcaac cccatcgtgc ctgtcgacct
    12481 ggtcatcgac cattcggtgc aggtcgacca gttcggttcg gccttcgcgc tcttcttcaa
    12541 cgccgagcgc gaatttgagc gcaatcgcga gcgctatgag ttcctcaaat ggggccagag
    12601 cgcgttccag aatttccgcg ttgtcccgcc ggccaccggc atcgtccatc aggtcaacct
    12661 tgaatatctg ggcaaggtcg tgatgaccaa acccgaaggt gacggattgg tagccttccc
    12721 cgatagcctg gtcgggaccg actcgcacac gacgatgatc aacggcctgg gcgtgctcgg
    12781 ctggggcgtc ggcggcattg aggccgaggc tgttatgctc ggtcagccga tttacatgct
    12841 cacgcccgaa gtcgtcggcg ttcgcctgac cggcagcctg ccggaaggaa ctactgccac
    12901 cgaccttgtt ttgcgcatca ccgaaatgct gcgcaagaag ggggtcgtcg gcaaatttgt
    12961 ggagtacttc ggccccggcc tgagcaacat gaccctggcc gaccgcgcga ctatcgccaa
    13021 catgtgcccg gaatatgggg ctacggtagg ctttttcccg gtcgacaacg agacattgaa
    13081 ctacatgcgc aacaccggcc gctcggccga gctgatcgat ctcgtcgagc gttacaccaa
    13141 ggaacagggg ctgttccgca cggattcgac tcccgaccct gatttcaccg atgtagtcga
    13201 gctggatctg agctccatcg agcctagcct cgccggaccc aaacttccac aggatcgcat
    13261 cccgctcaag aacatgaggg agtccttcca tcagagcctg gtcgctccag tcgggccgcg
    13321 cggctttggt ctgggcgaaa aagatttgga ccgcacggcc gaaatctcca acggtcacac
    13381 atcggagata ggccacggcg cagtcgtgat tgctgccatc accagttgca ccaacacctc
    13441 gaaccccagc gtcatggttg ccgcaggtct actggccaag aaggcagtcg aggccgggct
    13501 gacggtgccg ccctacgtga agaccagcct tgcccccggt tctcgcgtgg tcgaggatta
    13561 tttgaagcgc gccgggttgt tgccctatct ggagcagttg gggttctata tcgtcggctt
    13621 cggttgcaca acctgcatcg gcaactccgg ccccctgccg gaagcagtca gccaggctat
    13681 ccatgagagc gatctcgtcg tctcctcggt actcagcggc aaccgcaact tcgagggtcg
    13741 tgtccatccc gatacccgca gcaacttcct ggcttcgccg cccctcgtca ttgcctttgc
    13801 cctggccggc cgcgccgacc tggatatgta caacgaaccg ctcggcaacg gccgtgatgg
    13861 gcagccggtc tatctccgcg atatctggcc caccaacaaa gagatcagcc gcgtcatgac
    13921 cgagtcgctg acgccggaaa tgttcgagca cgaatacggc aacgtcttca ccggcaacga
    13981 aacgtggaat ctcatcccga ccggagacgg cgaactttat acgtgggatg agacatcgac
    14041 ctacattcag gagccgccgt tcttcgccgg tctttcccac gaagtgaacg ccatcaccaa
    14101 tatcgagggc gcgcgcgttc tggtgaaggt gggcgactcg atcactaccg atcatatctc
    14161 cccggccggc tccatctctc gcaccagccc ggccgcgcat tacctgacca accacgaagt
    14221 tgagccgcgc gatttcaact cctacggatc gcggcgcggc aatgatcgcg tgatgacccg
    14281 cggcaccttc gccaatatcc gcctgcgcaa ccagatggct cccggcaccg agggcggcat
    14341 gacgacctat ctgccgaccg gcgaagtcat gaccatcttc gacgcttcgc tccgctacaa
    14401 ggaaaccggc acgccgctcg tcgcgctggc cgggcgcgaa tatggaaccg gcagttcccg
    14461 tgactgggcg gccaaggggc cgttgctgct gggcatccgc ctggtgatnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tcccgtgact gggcggccaa ggggccgttg
    14641 ctgctgggca tccgcctggt gatcgccgag agcttcgagc gcattcaccg cagcaacctg
    14701 gttggcatgg gtattttgcc gctgcaatac aaggtcggcg aaaacgcaca aacgctggga
    14761 ttggacggcg cggagacctt ctccaccctt aaccttgatg atgatctgaa accaggacaa
    14821 accgttcgag tgcgggcaga gcatccggat gggcacgtag tggagttcga aaccatttgt
    14881 cgtatcgaca cgcctgtaga ggttgattac taccgcaacg gtggcatcct ccacacggta
    14941 ttgcgccagt ttttagccaa ctaacgtatg agatttccga caatccgtcc tgttcagggt
    15001 cgggcgaaac ttcgcccggc cctacttctg gcgatcatcg cccttgtcct gctgccggcg
    15061 gcatgccggc agcaacccgt tgtccccgaa agcaacaacg ggaccgccat gcatcgcgcc
    15121 aacccggcgc gaaccggagt ctaccagacg aaaggcccga cgaagtacga gtcgatcaaa
    15181 tggcagttcg aggccgacga ttgggtcttt ggcgcgccgg cggtcgccaa tggcaccgtc
    15241 tacttcacca gctacgatgg gcgcgtctat gctgccgacg cggagacggg cgccgaaaaa
    15301 tggcgcgttg atacgggaac taccattatc gcttctccgg ccgtgtccaa tgggctggtc
    15361 tatgtggcca acatggccgg ctctctcttg gccctcgatg ccgggagcgg gcaggaacgt
    15421 tggcgcgtgg agacggagac tggtcattcc ggctcacccg ccgttgtcga cggcgtcgtt
    15481 tacttcggtg gcgaaagcgg cctgctggtc gcgctcgacg ccgatacggg cgaagaacgg
    15541 tggcgcttcc agattcccgg cacggtgatc gccttctcga cagccgtcgc cgatgggctg
    15601 gtgttcgtgc cggtctccaa tggcgaactc catgtgcttg atgccgccac gggtgaagaa
    15661 caatggaagt tcgaccccaa cagcagcgac aacatttaca gtcccacggc tgatcccgtt
    15721 gtccataatg gatccgtcta tttcatggtc agtgatatca atctgacagg cgtgttgttc
    15781 gccattgatc tgaacaccca ccagctgcgc tgggagcaca agacttcgac ggaaaacttc
    15841 tcggcgcccg ccattctcga tgacatcctg gtctggggca gtctggaagg tatcctctac
    15901 ggcgtatcgg ccaccgacgg cgaagtgctg tggactttcc ccaccgacga catcatcttc
    15961 tctgccccag ccatcgcgaa tggcgtcgtc tacttcggta gtcacgacaa gaacctctat
    16021 gccgtggact accgaaccgg caaacaactg tggcaatttc gggcagagtc cggcgtctcg
    16081 tcaccggcaa tcgacaacgg catcgtctat gtaggcacac tggatggcgc tttctacgcg
    16141 cttcaataac ctgatataaa gctcaattaa atccaaggag ttaacatgaa tcacgatcac
    16201 gactcagagc ctgaagtatt ggcggaa
//
LOCUS       DAOMQG010000036        16130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1570_length_16130_cov_10.427932, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000036 DAOMQG010000000
VERSION     DAOMQG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1570_length_16130_cov_10.427932"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLC06_03740"
     CDS             <1..222
                     /locus_tag="PLC06_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HRO89588.1"
                     /translation="LAILVAWPIFFVQFHELLFPPGTWTFAYSDSLIRLFPEQFWFDF
                     GVLISGATLVLGVVVTLIGYVMSRRTARA"
     gene            complement(248..1336)
                     /locus_tag="PLC06_03745"
     CDS             complement(248..1336)
                     /locus_tag="PLC06_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRO89589.1"
                     /translation="MFFQKRKIGGLFLVAGLLLLVSLAACRPARTEVAEIAEPSPTAE
                     SAAPAPVVPPTLGQPQPVIVATIAAPPSPTSAPTATPTIEALSTPGTEEIPPTETPQP
                     TFTPPPPPPAVAGEQLWLQRPVPMSGPAWTDKAYPYGSTRGGVLRPHTGVEFVVPVGT
                     PVLAVSSGTVVVAGNDATVAYGPQENFYGNLVVIELDSDGATPLFALYGHLSEVLVAP
                     GQRVARGDVLGLSGASGVADGPHLHLEVRVGQNDYAATRNPLLWLEPLPQTGVVAGRI
                     IAPDGQLLFEAPISLVRVDATAPYTATTSYAQGEPNSDLTLGENFVMDDVVPGFYQAI
                     VETGGRRFTADLWVYPGRVNWVELVVGQ"
     gene            complement(1338..2870)
                     /gene="ftcD"
                     /locus_tag="PLC06_03750"
     CDS             complement(1338..2870)
                     /gene="ftcD"
                     /locus_tag="PLC06_03750"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRO89590.1"
                     /translation="MQKIVECVPNFSNGRDPEVYGRIVDAIRSVSAVQVLDVSADPDH
                     NRTVVTYVGPPDDVVEAGVRGIAAAKELIDLDHHEGEHPRIGATDVFPFVPVRGITMD
                     DCVMLARQLGKRVGEELGIAVYLYASAATRPDRESLSAIRKGQYEKWKEEVSTNPDRK
                     PDFGPAEPHPWGATVIGARPFLIAYNLYLNSSDVSVAESIARAIRYTSGGLRFVQARG
                     FLVDGQAQVSMNLTNFERTPIHRVQETVRREAERHGLTVTRAELVGLIPQKALLESAK
                     WYLQLDGLEDDQVLELRLTTPPAEEKPEETLSTIARPFVDSVAAPTSTPGGGSVAALV
                     GALAAALAQMVAGLTVGRKKYLDVSDQAAGILDEADSIRRELTAAIEEDSASFEGILA
                     VFRNKELGEEEKAIALEAATTHAGEVPLRVARLSRDASKLAKTIATIGNVNAATDAAV
                     GTIMARAAVHGAGLNVKINSVNLKNRELADRWAAEVVSLIAEAEELAQSTIAIAKERG
                     GF"
     gene            complement(2925..4640)
                     /locus_tag="PLC06_03755"
     CDS             complement(2925..4640)
                     /locus_tag="PLC06_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRO89591.1"
                     /translation="MSRHRTIRNKGVIAAARLQRSPAPGKRLAFRVDPWTRKLIRPLM
                     ISLLAVSVAVALLVIARIISPGPAWMALIPLCFLTALEGAYTSAWLNNPDSNGVDRLS
                     YRAAEVLLILVVARVYSWIVFVGGIPSPDEMRVFLVSPMAVLSTGGFISTAVITLAAW
                     WLAVTLSRIFTRLDVSIYEINFYTLPSIEQKAQADNQPIQTPREELQDQFLNTWLLAG
                     MGMVFLAALSTFEVNELTNVTNPFEITRLGLTPAMLFALMIYFLAGFWLLSHARLLRM
                     NARWLIDGVAKEADLERAWQRSTIVVLGLVALIAAFLPIGSTLAISQILSLGLAGIGY
                     LMNLFISFFGHLTAAALILLTQNVEETDPLMAQPTPAPLPTPVPAPPAAPNEFLTMAG
                     SSAFWALGVAIVIASLLFLLRERGYRLNRTVVGTYWSMLAGWARELWERLSGRARSLS
                     HELQNRLKRPTSLDIPHPDLKIRPPRFLRLGELSPREQIRFYYLALLRRAAESGIDRR
                     DSETPLEFAQEMKDAWPDSGTNIDALTEAFLEARYSPQPIDKHEAGSIKERWKSLRSR
                     LRKPR"
     gene            complement(4643..5935)
                     /locus_tag="PLC06_03760"
     CDS             complement(4643..5935)
                     /locus_tag="PLC06_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRO89592.1"
                     /translation="MSSRFRWLFLLLLGVAFLLRVDFIFYILYVIAGVFLWSWWRGGR
                     TLQRIVASRTYRKRAFLGEEVEVKLALTNTSRLSVPWVEFHESVPPELRLESAVHQVA
                     NLRGHRSISFTYRVKGLQRGYYRLGPLRLTSGDLFGLVKPQAGILRSDFLTVYPKIVQ
                     LTQLGLPSRLPFGTIAGRQRLFEDPARPMGAREFRSGDSLRQMNWKASARNQKMLVRT
                     FEPAISLETAILLDMNAAAYERRNRYDSIEWGIVTAASLATHLINQRQAVGLNTNGID
                     PLRLQEESRVFDETTGRLLFESTQGSNDLSRFTPPAIEARAGRPHLMKVLELLARLYA
                     RESTPFAEWAPAACVNLSWGVTVIAITAQGDPHTCNTLHHLVRSGFNPILIAVEPDAN
                     FSQVRERARRLGFRAFNVVDRRDLAQWQRPLHTLHGGG"
     gene            complement(5935..6879)
                     /locus_tag="PLC06_03765"
     CDS             complement(5935..6879)
                     /locus_tag="PLC06_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRO89593.1"
                     /translation="MDDIRAFSQSVLDNVERVIIGKRDVLELLMVSILCEGHILLEDV
                     PGTGKTMLARALAASLAIDFKRLQCTPDLLPNDVTGVSVYDPKRQEFVFIPGPVFTNI
                     LLADEINRATPRTQSALLEAMGERQVSVDGVTRNLERPFLVIASQNPVEYEGTFPLPE
                     AQLDRFFMKLSLGYLDEAEESRMLQNLSGEHPIESLRPVVDGHELPALTRKIWQIHVD
                     DTIRDYIVALAFATRRHPDLILGASPRGSHNLYRAAQAFAALQGREYVLPDDVKKLAP
                     AVIAHRCLLHPESALRGATVIDIIAAIVEETPLALGEV"
     gene            complement(7011..8261)
                     /locus_tag="PLC06_03770"
     CDS             complement(7011..8261)
                     /locus_tag="PLC06_03770"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HRO89594.1"
                     /translation="MRYSTFGEKFVRECGILQLMDDLGHALAEGGDDVIMLGGGNPSR
                     IPAVEACLRQRMETLLTQGDTFERLVGNYAPPAGDRPFRRAVAELLRREFGWPVTEDN
                     IALTNGSQTACFYLFNMFAGQYANGTRKKILLPLTPEYIGYTDAGIDDDIFISYRPEI
                     EYLENGLFKYRVDFDALTITDEIGAICVSRPTNPTGNVLTDEEIDRLHQMAKQAGIPL
                     IIDNAYGLPFPGILYRDVRPVWDEQIVFCFSLSKLGLPGARTGIIVAAEPIAAAIAGA
                     NAILSLAPGNFGAYMALDLMESGDVIRLSREAIRPHYQHKAELALAAAREEMAGLNVH
                     IHDPEGSFFLWLWFEDLPITSAELYQRLKARGVLVVPGHYFFPGLPGEWRHRHECLRM
                     NYAGDDRAVRRGLAIIADEVRAAG"
     gene            complement(8258..9457)
                     /gene="rocD"
                     /locus_tag="PLC06_03775"
     CDS             complement(8258..9457)
                     /gene="rocD"
                     /locus_tag="PLC06_03775"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRO89595.1"
                     /translation="MNSQDYMKLDDMYAAHTYHPIEVVIERGEGPWVYDVDGNKYLDC
                     LSAYSAVNQGHCHPRILEAAMTQMHKVTLTSRAFYNDQMGPFLKQLCELTGYEMAVPM
                     NTGAEAVETAIKIARKWGYQVKGVPDNQAEIIVCEGNFAGRTISIISFSSEEQYKQDF
                     GPLTPGFKIIPYGDADALAKAITPNTVAFLTEPIQGEGGVNVPPAGYLKRVAEICAAN
                     NVLFMADEIQTGLGRTGALLCSDHEGVRPDVVTLGKALSGGFYPVSAVLADKRVLGLF
                     RPGDHGSTFGGNPLGSAVARAALDVLVDEGLVDRSREIGAYFMGRLKRIETEHIKEVR
                     GKGLLIGVEMTNQARPYCDALRDAGILCKDTHGTVVRFAPPLVISKEEIDWAMDRIEP
                     ILSNGHQ"
     gene            complement(9684..10493)
                     /locus_tag="PLC06_03780"
     CDS             complement(9684..10493)
                     /locus_tag="PLC06_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRO89596.1"
                     /translation="MSLLEAIIIGIIQGATEFLPISSDGHLVLIPAIFGLSQPDLVLI
                     GLVHAGTLLAIISYFARDLWAILKAVINGLVQRRPFADDNARLGWLMVLGSVPAGIVG
                     LALKDWFEQQFESPVIAAAGLLVTAAFLVVGERLLRGVKTIPQLTWLDTLIIGSFQVL
                     ALLPGVSRSGTTIAAGLGRGLDRPSAARFSFLVGLPAIAGAGLLSIIDIFTEQGSLPM
                     GHYVAAFTAAAVVGYLCIAFLLSWLKSHSLYLFATYCATVGTLYLLYSFLF"
     gene            complement(10590..11708)
                     /gene="tgt"
                     /locus_tag="PLC06_03785"
     CDS             complement(10590..11708)
                     /gene="tgt"
                     /locus_tag="PLC06_03785"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRO89597.1"
                     /translation="MSFEFTSLAQDGAARLGRFETPHGPIETPVFAPVGTQGTVKAMR
                     PRDLVELGASLVLANTYHLYLRPGDERVRDLGGLHRFMGWDGPILTDSGGFQVFSLSD
                     ARKIDADGVTFKSHLDGSFHRFTPEKSIAVQENLGADIIMMFDECPPPNEYDYVRQSL
                     ERTHPWAERCLAAKTRPDQALFGIVQGGVFRDLREESARFLIDLDLPGYAVGGLAVGE
                     SKPEMHAVLEWLHPILPADKPRYLMGVGAPEDLVNGVLRGIDIFDCVLPTRIARNGAA
                     LVLGGRINLRNAQYTDDPRPVDPGCDCYACTHFSRAYIRHLVKAEEILASILLTTHNL
                     HFLLRLMDDLRKAIRKGTVAEYAAEFLSRYEIRDTRYE"
     gene            complement(11716..>12192)
                     /locus_tag="PLC06_03790"
     CDS             complement(11716..>12192)
                     /locus_tag="PLC06_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO89598.1"
                     /translation="DVTGMYGGYIGEGTKTVLPSRAHAKISMRLVPDQDPAEILQLFE
                     RFVRSVLPPSITLNILHSGGGKAALVDRDTAPMRAAAAAYEAAYQRPPVFMREGGSIP
                     VVNQFETDLQLPTVLMGFGLPGDHIHSPNERFYVPNFYKGIETSIHYLDLLSNGSK"
     assembly_gap    12193..12292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12293..12825)
                     /locus_tag="PLC06_03795"
     CDS             complement(<12293..12825)
                     /locus_tag="PLC06_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO89599.1"
                     /translation="MSIPLQYANDHQQAHLGELMDFLRFPSVSTLAARNSDTAATANW
                     LAQAMTNAGLENVQVIPTKRHPLVYADWLHAGVEAPTVLIYGHYDVQPAEPLELWETP
                     PFEPTIRDDYLYARGASDDKGQVYIHVKAVEAYLRTIGRLPVNVKFIVEGEEEIGSRN
                     LHEFIPANRELLAADSAL"
     gene            12927..13469
                     /gene="scpB"
                     /locus_tag="PLC06_03800"
     CDS             12927..13469
                     /gene="scpB"
                     /locus_tag="PLC06_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRO89600.1"
                     /translation="MSESSALNGVTMAATPLMQLESLLFVASGPVSPARLATALEMTP
                     SAVAELLRELDADYGTRGLRLQWSGGGLVQLTTAAETATAVERFLGLELTTRLSQAAV
                     EVLAIVAYLQPATRPQIDQIRGVNSDGALRSLLSKGLIEEVGRLEKPGRPILYGTTPD
                     FLQSFGLGALTDMPPLDEEE"
     gene            13540..14364
                     /locus_tag="PLC06_03805"
     CDS             13540..14364
                     /locus_tag="PLC06_03805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRO89601.1"
                     /translation="MQKIMARANIGSRRSCELIISEGRVTVGGRVARLGDKADPDHDR
                     IAVDGRLLFADRNEEGAQTGLIYIALNKPRGVISSLEDELEVGRTTVRDLIEIEGEHH
                     IYPVGRLDKPSEGLVLMTNDGALAHRLTHPRYGHEKVYDVTVEGDISEELLDQWRHGV
                     VLDGRITAPAPIEVVERTQQATRLRITLREGRKRQIRRIAAALGHPVQRLIRERIGPL
                     ALGDLPPGQWRYLTEREISKLKRSAFDPSTAAGSTPQNKPLRYAKHSNRKRETRAA"
     gene            14354..15067
                     /gene="cmk"
                     /locus_tag="PLC06_03810"
     CDS             14354..15067
                     /gene="cmk"
                     /locus_tag="PLC06_03810"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRO89602.1"
                     /translation="MQPDYLPDVIAIDGPAASGKSTIGLMLAQRLGYLCLDTGSMYRA
                     LTLAAIWHGISPDDEEAIVQLAIDCDLDILPLSGETDGRHYTVLLEGVDVTWDLRLPA
                     VDASVSQISTYPGVRAEMVRRQREVGRRGHVIMIGRDIGTVVMPDAALKLYIIASPEE
                     RARRRLLDRRRQGHDDDYESILADVARRDAIDSGRKHSPLRPAADAIQIDTTGRTAEE
                     VIAEIMALIDQTQPEGRDS"
     gene            15067..>16130
                     /locus_tag="PLC06_03815"
     CDS             15067..>16130
                     /locus_tag="PLC06_03815"
                     /inference="COORDINATES: protein motif:HMM:NF039894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRO89603.1"
                     /translation="MALFNEIDPNTFSGGQIIGIEPGSVAAALGLRPGDELLAINDQA
                     VEDIIDVQFYAAEEELELLVRREGEVLLFEGERNYNQTLGLEFAHPTFDVDIRRCDNL
                     CEFCFVLQMGPKYRRTLYVKDDDYRYSFLFGHYVTLTNLNDHDWWRIENMRLSPLYVS
                     VHVTDTETRRRFLRNPNAGDIMEQLRWLAEHHIEVHAQLVIVPGFNDGEWLAQSVKDL
                     ATLWPDDDVVGGVVSVSVVPVGLTKFHKYGMRPHTREEALATLEFVESLQQEYLDDFG
                     IRFVYLTDEWYLVTGRDVPPLEDYDGHDLHENGLGMVRAFLDDWIVVSDEIAHTEPKP
                     APRTITLATAALFAPTLRQKA"
BASE COUNT         3159 a         4618 c         5108 g         3145 t
ORIGIN      
        1 ttggctattt tggtcgcctg gccgatcttc ttcgttcagt tccacgagct gctctttcca
       61 cccgggactt ggaccttcgc ctatagtgat tcgctgattc gcttgtttcc ggagcagttc
      121 tggttcgatt tcggggttct gatcagcggg gcgacgctgg tattgggcgt tgttgtcact
      181 ctgattggct atgtgatgag ccgccgcacc gcgagagcgt aattattcgc cggccaactg
      241 atggaaatca ctggcccaca accagttcaa cccaattgac ccggcccgga taaacccaca
      301 agtcggccgt gaagcgacgg ccgccggtct cgacgatggc ctgatagaag cccggaacga
      361 catcatccat gacgaaattt tcgcccaggg tcaaatcact gtttggctct ccctgggcat
      421 aagaggtcgt ggcggtatag ggagcggtgg catccacccg caccagcgag atcggcgctt
      481 cgaacaacaa ctgaccgtcc ggggcgatga tccggccggc gacgacgccg gtttgaggca
      541 acggttccag ccacaacaga ggattgcgag tggcggcata gtcgttttgg ccgaccctga
      601 cctcaagatg caggtgtggc ccgtcggcca cgccactggc tcccgagagg cccagcacat
      661 caccacgcgc tacccgctgg ccgggcgcaa caagcacttc ggacaggtgg ccgtagagcg
      721 cgaacaatgg ggttgcgccg tcgctgtcca gctcgatgac gacgaggttg ccgtagaagt
      781 tctcttgtgg cccataagcc acagtcgcgt cgttcccggc gacgacgacc gtgccggacg
      841 agacagccag aaccggcgtc ccgacgggca cgacgaactc gacccccgtg tgcggccgaa
      901 gtacgccgcc gcgcgtgctg ccatatgggt aagccttgtc ggtccaggcc gggccgctca
      961 tcggcaccgg ccgctggagc catagctgct cacctgcgac agccggcggt ggcgggggtg
     1021 gggtgaaggt tggctggggt gtctcggttg gcggtatttc ctcggttccc ggtgtggata
     1081 gggcttctat cgttggcgtg gcggtgggag cgctggtcgg tgagggcggg gccgcgatag
     1141 tcgctacgat gaccggctga ggctggccca gggtgggggg cactaccgga gccggcgcgg
     1201 ccgattcggc ggtcggagat ggttccgcga tctctgcaac ctcggtccgt gccgggcggc
     1261 aggcagccaa agagacaagc aatagaagac cggcgacgag aaacaggccg ccgatctttc
     1321 ttttttggaa aaacaatcta aaagcccccg cgctccttgg cgatggcgat ggtcgattga
     1381 gccaattcct cagcctcggc gatcaaggaa acgacctcgg cggcccatcg gtcggccagt
     1441 tcacgattct tcaggttgac gctattaatc ttgacgttca acccggcgcc gtggaccgcg
     1501 gcacgggcca tgatggtgcc cacggccgcg tcggtggcgg cgttgacgtt gccgatagtg
     1561 gcgattgttt tggcgagctt gctcgcgtcc cggctcagcc gcgccacgcg aaggggaact
     1621 tcgccggcgt gggtggtggc cgcttccagc gcgatcgcct tctcttcctc gcccaattcc
     1681 ttgttacgaa aaaccgccag aatcccttcg aaggacgcgg agtcttcttc gatggccgcg
     1741 gttaattcgc gacgaatgga atcggcctcg tcgagaattc cggcggcctg gtcgctgacg
     1801 tcgaggtatt tcttgcggcc gaccgtcaac ccggcgacca tctgcgccaa ggcggcggcc
     1861 agtgcgccga ccagcgcggc cacggaaccg ccgccgggag tcgacgttgg ggccgccacg
     1921 gaatcgacga atggtctcgc gatggtcgac agggtttcct ccggcttttc ttcggcgggc
     1981 ggcgtcgtca ggcgcagctc caatacctga tcgtcttcca gcccatcgag ttgcagatac
     2041 cacttggcgc tttccaggag ggccttttgg gggatcagcc cgaccagttc ggcccgcgtc
     2101 accgtcaggc catggcgctc ggcttcccgc cggacggttt cctgcacgcg gtggatgggt
     2161 gtgcgctcga aatttgtcag attcatgctg acctgcgcct gcccgtcaac gagaaagccg
     2221 cgtgcctgca cgaagcgcag cccgccgctg gtatagcgaa tggcgcgggc gatactttcg
     2281 gcaacgctga catcacttga gttgaggtag aggttgtagg cgatcaggaa cggccgcgcg
     2341 ccaatcacgg tagctcccca tgggtgcggc tcggccggcc cgaaatccgg cttgcggtcg
     2401 gggttggtgc ttacttcttc tttccacttc tcgtattgtc ccttacggat agcggacagg
     2461 ctttcgcgat cgggacgggt ggctgccgag gcgtacaggt acacggctat gcccagttcc
     2521 tcgcccacgc gcttgcccaa ttggcgagcc agcatcacgc agtcgtccat cgtgatgccg
     2581 cgcacgggga cgaagggaaa cacgtcggtc gccccgatgc gcgggtgctc gccctcatgg
     2641 tgatcgagat cgatgagttc cttggccgcg gcaatgccgc gaacaccggc ttcgacgacg
     2701 tcatcgggtg ggccgacata ggtgacgact gtccggttgt ggtcggggtc ggcgctgaca
     2761 tccagcacct gaacagcgga aacgctgcgg atggcatcga cgatgcggcc gtagacttct
     2821 gggtcgcggc cgttactgaa gttgggaaca cattccacaa tcttttgcat aactatctcc
     2881 tgatgtttga gggagtttta tcagcagtcg gcgctttcgg ctacttagcg cggcttgcgg
     2941 aggcgacttc tgagcgattt ccaacgttcc ttgattgagc ctgcctcgtg cttgtcaatc
     3001 ggttgcgggc tatagcgggc ttccaggaaa gcctcagtca acgcatcgat gtttgtcccg
     3061 gaatcgggcc aggcgtcttt catctcctga gcaaattcca gtggcgtttc gctgtcacgc
     3121 cgatcaatgc cgctttcggc cgcgcgtcgc agaagggcca gataatagaa gcggatttgt
     3181 tcgcggggcg agagttcgcc gagccgcagg aagcgcgggg ggcgaatctt caggtctgga
     3241 tgaggtatgt ccagcgaggt cgggcgtttt agccggttct gtagctcatg gctcagcgag
     3301 cgtgcgcggc cgctcagccg ctcccatagt tcccgtgccc agccggcgag cattgaccaa
     3361 tatgtgccaa ctaccgtcct gttaaggcgg tagccccgct cgcgcaacaa gaacaggagt
     3421 gacgcaatga cgatagccac gcccaaagcc caaaatgcgg aactgccggc catggtcagg
     3481 aactcattgg gggcagcggg aggtgcgggt acgggcgttg gcaggggggc aggtgtcggc
     3541 tgggccatca atgggtcggt ttcctcgaca ttctgcgtca gaagtatcaa tgccgcggcc
     3601 gtcagatgac caaaaaagga gatgaacagg ttcatcagat acccgattcc ggccagcccc
     3661 agggacagga tttgactgat agccaatgtg gagccgatag gcaggaaggc ggcgatgaga
     3721 gcgacgaggc ccaacacgac gatcgtgctg cgttgccagg cgcgctccag atcggcttcc
     3781 ttggcgacgc catcgatcag ccagcgcgcg ttcatccgta acagccgggc atgactaagc
     3841 agccagaacc ccgccaggaa gtagatcatc agagcaaaga gcatcgccgg cgtcaggccc
     3901 agccgggtga tctcgaacgg gttggtaaca ttcgtcagtt cattgacttc gaatgtgctg
     3961 agagcagcca gaaagaccat gcccatgccg gcgagcagcc acgtgttgag aaactgatcc
     4021 tgtaactcct ctcgaggagt ctgaatcggc tggttgtcgg cctgagcctt ctgctcgatc
     4081 gagggcaggg tgtagaaatt gatttcgtaa atgctcacgt ccaaacgggt gaagatacgg
     4141 ctcaacgtga cggcaagcca ccaggccgcc agggtgatca ccgccgtcga aatgaacccg
     4201 ccggtagaca ggacggccat cggcgagaca agaaatacgc gcatctcgtc cggcgagggg
     4261 atgccgccaa cgaagacgat ccagctatag acgcgggcaa cgaccagaat gagcaagacc
     4321 tcggccgcgc gatagctgag gcgatccacg ccgttgctgt cgggattatt gagccaggcg
     4381 gacgtatagg ctccttccag cgcggtcaga aagcaaaggg gaattagcgc catccaggcc
     4441 ggccccggcg aaatgatccg cgcgatcacc agcagcgcga cggcaacaga gacggccaac
     4501 agagagatca tcaacggccg gatcagcttt cgggtccatg gatcgacgcg aaacgccagc
     4561 cgtttgccgg gggcgggcga gcgttgcagg cgcgcggcgg cgataacgcc cttgttacgg
     4621 atagtacgat ggcgactcat cgttagcccc ctccgtgcaa ggtgtggaga ggtcgctgcc
     4681 actgggccag gtcccggcga tcgacaacgt tgaaggcgcg aaaacccagc cggcgcgccc
     4741 gctcgcgcac ctgactgaaa ttggcgtcag gttcgacggc gatcaggatc gggttgaagc
     4801 cgctgcggac gaggtggtgc agggtgttgc aggtgtgggg atcgccctga gcggtgatgg
     4861 cgatgacggt gaccccccag ctcaggttga cgcaggccgc cggggcccat tcggcaaaag
     4921 gagtcgattc acgtgcatag aggcgagcca acagctccag caccttcatc aaatgcggcc
     4981 gaccggcgcg cgcttcgatc gccggaggcg taaatcggga tagatcattc gatccttgag
     5041 tcgattcaaa gagcaaccgg ccggtcgtct cgtcgaacac tctggattct tcctgtaagc
     5101 gaagcggatc gatcccgttg gtattcagac ctaccgcctg gcgttggttt atcagatggg
     5161 tggccagcga ggcggcggtg acaatgcccc attcgatgga atcgtaccgg tttcggcgct
     5221 cgtaggcggc cgcgttcata tcgagcagga tggccgtttc cagcgagatg gccggttcga
     5281 aggtacggac gagcatcttt tgattgcggg cactggcctt ccagttcatc tgccgcaatg
     5341 aatcgccgga gcgaaattcc cgtgcgccca tcggccgtgc cgggtcctcg aacaggcgtt
     5401 ggcgcccggc gatcgtgccg aaaggcagcc gggaaggaag ccccagttgg gtgagttgga
     5461 caatctttgg gtagacggtc aggaaatccg accgcagaat gcccgcctgg ggcttgacca
     5521 ggccgaacag gtcgccgctc gttaatcgca gcgggccgag gcggtaatag ccgcgctgga
     5581 gtcctttcac tcggtaggtg aacgagatcg atcgatggcc gcgcaggttg gcgacctgat
     5641 gcaccgccga ttccagccgt aactcgggcg gtacgctctc gtggaattcc acccagggga
     5701 cggaaagccg gctggtgttg gtcagcgcga gcttaacctc cacctcttcg cccagaaagg
     5761 cacgcttgcg ataggtgcgc gacgccacga tacgctgcag ggtgcggccg ccgcgccacc
     5821 aactccagag gaagacgccc gcaatcacat agagaatata aaagataaaa tcgacccgaa
     5881 ggagaaaggc aacccccagc agcagcagaa agagccaccg aaagcgggac gacatcagac
     5941 ctctccgagc gccagcgggg tctcctcgac gatagcggcg atgatgtcga tgacggtcgc
     6001 gccacgcaat gcgctctccg ggtggagcag gcagcgatgg gcgatgactg cgggcgccag
     6061 ctttttcacg tcgtcgggga gcacgtactc cctgccttgc agcgcggcga aggcttgcgc
     6121 ggcacgatac aggttatgac tgccgcgcgg gcttgcgccc aaaatcaggt cgggatgccg
     6181 cctcgtcgcg aatgccagcg ccacaatata gtcgcggatg gtgtcatcta catgaatctg
     6241 ccagatcttt cgagtcaggg cgggcagctc atgcccatcg acgaccggcc tcagcgattc
     6301 gataggatgt tcgccgctca gattttgcag catccggctt tcctcggctt catcgagata
     6361 gccaagcgac agtttcatga agaagcggtc cagctgggct tcgggcaggg ggaatgtgcc
     6421 ctcatactcc accggatttt gcgaggcgat taccaggaag gggcgctcaa ggtttctcgt
     6481 caccccatca acgctgacct gtcgctcgcc catcgcttca agcaaggccg attgtgtgcg
     6541 gggcgtggcc cggttgatct cgtcggcgag caggatgttg gtgaagacag ggccggggat
     6601 gaagacgaac tcctgccgct tcgggtcata gaccgacacg ccggtgacgt cgttcggcag
     6661 caaatcgggc gtgcattgca gccgcttaaa atcgatcgcc agactggcgg ccagtgcgcg
     6721 ggccagcatg gttttgcctg tgcccggcac gtcttcgagc aggatatgcc cctcacagag
     6781 gatcgagacc atcagcaatt cgagaacatc tcgcttgccg atgatcacgc gctcgacgtt
     6841 gtccagtacg ctttgagaga aagcccgtat atcgtccata atgctcctgg cttcatctta
     6901 gcatagtcta ccctgactgg gtacaggatc gggcaacggg gaatggggag cttgggccgg
     6961 gatcgaaaaa caaacctcag aagcccgtgg gagcctctga ggtttgtcga ttagccggcg
     7021 gcgcggactt cgtcggcgat gatggccaaa ccacgacgga cggcgcggtc gtctccggca
     7081 taattcatgc gcaggcattc atgccggtgg cgccattcgc ccggcaggcc agggaagaag
     7141 taatgccccg gcacgaccag aacgccacgc gccttcaacc gctgataaag ctcggcggaa
     7201 gtgatgggca ggtcttcgaa ccacagccac aggaagaacg atccttcggg atcatggatg
     7261 tggacattca atccggccat ttcctcgcgc gcggcggcca acgccagctc ggctttatgt
     7321 tgataatgag gccggatggc ctcgcggcta aggcggataa cgtcgccgct ctccatcagg
     7381 tcaagcgcca tgtaagcgcc gaagttgccc ggagccaggc tgaggatagc gttggctccg
     7441 gcgatggccg cggcgatcgg ttcggcggcg acgatgatgc cggttcgcgc gccgggcaag
     7501 cccagcttcg acaggctaaa gcagaaaacg atctgctcat cccacaccgg tcgcacgtcc
     7561 ctgtagagga tgccagggaa gggcagacca taggcgttgt cgatgatgag cgggatgccg
     7621 gcctgcttcg ccatctggtg gagccgatcg atctcttcgt cggtcagcac attaccggtg
     7681 gggttggtcg gccgcgaaac acagatagcc ccgatctcgt cggtgatggt caatgcgtca
     7741 aaatcgaccc ggtatttgaa caggccgttc tcgaggtact cgatctccgg ccgataggag
     7801 atgaagatat cgtcgtcgat gccggcatcg gtatagccga tgtattccgg cgtgagcggg
     7861 agcagaatct tcttgcgcgt gccgttggcg tattgtccgg cgaacatgtt gaacaggtag
     7921 aagcaggctg tctggctgcc gttggtcagg gcgatattgt cttcagtgac gggccagcca
     7981 aactcgcggc gcagcagctc ggccacggca cgccggaagg gtcgatcccc ggccgggggt
     8041 gcgtagttgc ccaccagacg ctcgaaggtg tcgccctggg tgagtaaggt ctccatgcgt
     8101 tggcgcaggc aggcttcgac ggccgggatg cggctggggt tgccgccacc gagcatgatg
     8161 acgtcatcgc cgccctcagc cagcgcgtgg cccaggtcgt ccatcagttg caaaatgccg
     8221 cactcgcgga cgaacttctc gccgaatgtt gaatatctca ttgatgtccg ttcgacagga
     8281 ttggctcgat gcgatccata gcccagtcga tctcttcctt gctgatgacg agcggcgggg
     8341 cgaagcggac gacggtgcca tgagtgtcct tgcacaggat gccggcgtcg cgcaatgcgt
     8401 cacagtaggg gcgagcctgg ttggtcatct caacgccgat cagcagcccc ttgccgcgca
     8461 cttccttgat atgttccgtc tcgatgcgct tgaggcggcc catgaagtag gcgccgatct
     8521 ctcgggagcg gtcgacgagg ccttcgtcaa cgaggacatc gagcgcggcg cgggctacgg
     8581 ccgagccgag cgggttgccg ccgaaagtgc tgccatggtc gcccgggcgg aaaaggccca
     8641 gcacgcgctt gtcagccaga acggccgata cgggatagaa gccgccgctg agggccttgc
     8701 ccagggtgac gacgtcggga cgcacacctt catggtcgct gcacagcagc gcgccggtgc
     8761 ggccgaggcc ggtctgaatc tcgtcggcca tgaagaggac attattggcg gcgcagatct
     8821 cggctacgcg cttcaggtat ccggccgggg ggacgttgac gccaccctcg ccctggatag
     8881 gctcggtgag gaaggcgact gtgttggggg tgatagcctt ggccagcgcg tcggcgtcgc
     8941 catagggaat tatcttgaag ccgggggtca gcgggccgaa atcttgcttg tactgctctt
     9001 cactgctgaa gctgatgatg gagatagtgc gtccggcgaa attgccttca cagacgatga
     9061 tctcggcctg attatccggc acgcccttca cctggtagcc ccatttgcgg gcgatcttga
     9121 tggccgtttc gacggcctcg gcgccggtgt tcattggcac agccatctca tagccggtca
     9181 gttcgcacag ttgcttgagg aacgggccca tctggtcgtt gtagaaggcg cgcgaagtca
     9241 gggtcacttt gtgcatttgc gtcatggccg cttcgaggat gcgcgggtga cagtggccct
     9301 ggttgacggc cgaataggcc gagaggcagt cgagatattt gttgccgtcc acgtcataga
     9361 cccagggacc ctcgccccgt tcaatgacca cttcgatggg gtgataggta tgagcggcat
     9421 acatatcatc aagcttcatg taatcctggc tgttcataac tcattctcct gagccggtcc
     9481 tttcagtggg aagcggacct gatgtgaaat ggggtaactc gattaaaaaa agcctgactc
     9541 gttagaaatc aggctttgct cggacctacg aatcatacgc aggaaggtcg tcttgtataa
     9601 ggtttatgcg tatttcagct agttacatta tacaccagtt ggcatgtggc tgtcgaccgg
     9661 aaggaagaag gggatcaggc ttctcaaaac aggaacgaat acaacagata cagcgttccg
     9721 actgtcgcgc agtaggtggc gaagagatag aggctatggc tcttgagcca tgacagcagg
     9781 aaggcgatgc acagatagcc cactacggcc gcggccgtga atgccgccac gtaatggccc
     9841 atcggcagcg atccttgttc ggtgaatatg tcgatgattg acagcaggcc tgctccggcg
     9901 atagcgggca gcccgaccag gaagctgaac cgcgccgccg acggccgatc cagcccgcgc
     9961 cccaatccgg cggcaatggt cgtgccgctg cgcgagacgc ccggcagcag cgccagcacc
    10021 tgaaatgagc cgatgatcag tgtgtccagc caggtgagtt gaggaatggt cttgacgccg
    10081 cgcagcagcc gctcgccgac caccagaaac gcggccgtga cgagcaaccc tgccgcggcg
    10141 atgaccggtg actcgaattg ctgctcgaac cagtctttca gcgccagccc gacgatcccc
    10201 gccggcaccg agccgaggac catcaaccag cccagccggg cgttatcgtc ggcgaatggc
    10261 ctacgctgga cgagcccgtt gatgactgcc ttgaggatgg cccacaagtc gcgggcgaaa
    10321 tagctgatga tggccagcag cgtcccggcg tggacgaggc cgattagcac caggtcgggc
    10381 tggctcaggc caaatatggc cgggatgagt accagatggc cgtcgctgga aatgggcagg
    10441 aactccgtcg cgccctgaat aattccgatg atgatcgctt ctagtaatga catagtcgta
    10501 aatgaaccga ttgcttgagg ttatctgacg gcgagccacg ggccacagca aacaagagga
    10561 gctgcggctt gtcgctcgca gtctcctctt tattcgtatc tcgtatctcg tatctcgtat
    10621 ctggataaaa actccgctgc atactctgcc actgtcccct tgcgaattgc ctttcgcaag
    10681 tcgtccatca ggcgcagcag aaaatgaagg ttgtgggtcg tcagcaagat ggacgccaga
    10741 atctcttccg ccttcaccag gtgacggata taggcccggc tgaaatgggt gcaggcataa
    10801 cagtcgcacc ctgggtctac cggccgtggg tcgtcggtgt actgggcatt gcgcaagttg
    10861 atccggccgc ccaataccaa cgccgccccg ttgcgggcga tgcgcgtcgg cagcacgcaa
    10921 tcgaagatgt cgatgccgcg cagcacgccg ttgactaaat cctccggcgc gcctacgccc
    10981 atcagatagc gcggcttgtc ggcgggcagg atcgggtgaa gccattccag cacggcgtgc
    11041 atctccggct tcgattcgcc gactgccagc ccgcccacag cgtaaccggg caggtcgaga
    11101 tcgatcagga agcgggcgct ctcctcgcgc aggtcgcgga atacgccccc ttgcacgatg
    11161 ccgaacagcg cttgatccgg ccgcgtcttg gccgccaggc agcgctcggc ccaggggtgc
    11221 gtgcgctcca gcgattgccg gacgtagtcg tactcattgg gtggtgggca ctcgtcgaac
    11281 atcatgatga tgtcggcccc caggttttcc tgcacggcga tcgatttctc cggcgtgaag
    11341 cgatgaaacg agccgtcgag gtgggatttg aacgtcacgc catcggcgtc gatcttgcgt
    11401 gcgtcgctca ggctgaacac ctgaaagccg ccgctgtcgg tcaggatggg gccatcccag
    11461 cccatgaagc ggtgcagccc gcccaggtca cgcacgcgct catcgcccgg ccgcagatag
    11521 agatgatagg tgttggccag caccagcgac gcccccagtt ccaccagatc gcgcggccgc
    11581 atcgctttta ccgttccctg cgtgcctacc ggggcaaaaa ccggcgtctc gattggcccg
    11641 tgcggcgtct caaatcgccc cagtcgcgcc gcgccgtctt gggccagtga tgtgaattca
    11701 aaagacattg agaacctatt ttgagccgtt actcaggagg tccaggtagt ggatgctcgt
    11761 ctcgatgccc ttgtaaaagt tgggcacata gaaccgctca ttaggcgaat gaatatggtc
    11821 gcccggcagg ccaaagccca tcagcacggt gggcagttgc agatcggtct cgaactgatt
    11881 cacgacgggg atcgatccgc cctcccgcat gaagacgggc ggccgctgat aggcggcctc
    11941 gtaggcggcc gcagcggcgc gcattggcgc tgtgtcccga tcgacaagcg cggctttgcc
    12001 tcctcccgaa tggagaatat tgagggttat gctgggcggc aaaaccgaac ggacgaagcg
    12061 ctcgaacaat tgcaggatct ctgccgggtc ctgatccggc accaggcgca tggatatttt
    12121 ggcgtgggcg cgggacggca acacggtctt ggtgccttcg ccgatgtaac cgccatacat
    12181 gccggtcacg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagggccga
    12301 atcggccgcc agcagttcgc gattggccgg gatgaattcg tgcagattgc ggctgccgat
    12361 ctcctcttcg ccttcgacga tgaacttgac attgacgggc agccggccga tcgtccgcag
    12421 ataggcctcg accgccttga cgtggatgta gacctgccct ttgtcatccg acgcgccgcg
    12481 cgcatagaga taatcgtccc gaattgtcgg ctcaaacggt ggcgtctccc atagctccag
    12541 cggctcggcg ggttgaacgt cgtaatggcc atagatgagc acggttggag cctcgacgcc
    12601 ggcatgcagc cagtcggcat acacgagagg gtggcgtttg gtggggatga cctggacgtt
    12661 ctccagcccg gcgttagtca tagcctgggc cagccaattc gcagttgcgg ctgtgtcgct
    12721 attacgcgcg gcaagggtgc tgacgcttgg gaagcgcagg aaatccataa gttcgcccag
    12781 gtgggcctgt tgatgatcgt ttgcgtactg tagaggtata gacatgatac attccaatgg
    12841 ggaaaggatg tgttattatg ggtataatac ccggttggtg gccgattgtc ctgttagacg
    12901 cgcgagccac tttagtgtga gtgagtatgt ccgaatcatc cgccttgaat ggtgtcacta
    12961 tggctgcaac ccctctcatg caattggaga gcttgttgtt cgttgccagt ggcccggttt
    13021 ctcccgcgcg tctggcgacc gccctggaga tgacgccttc ggccgtggcg gaactgctgc
    13081 gcgagctgga tgccgattac gggacgcggg gtttgcgctt gcagtggagc gggggcggcc
    13141 tggtgcaact caccacggcc gcggagacgg ccaccgccgt cgagcgcttc ctggggcttg
    13201 agttgaccac ccggctgagc caggcggccg tcgaagtcct ggccatcgtc gcctatctgc
    13261 agccggccac gcgaccgcag atcgatcaga tccgcggggt caattccgac ggcgcgttgc
    13321 gttccttgct gagcaagggg cttatcgaag aggtaggccg cctggagaaa cccggcaggc
    13381 cgatccttta tgggacgacg cctgatttcc tgcagtcatt tggcctgggc gcgctgacgg
    13441 atatgccgcc tctggatgag gaagaatgat gcgggtgatg agagcgttac ctttgcacct
    13501 gacatagttg cccgaaagag aaattctgtg gaagagagat tgcaaaagat aatggcgcgg
    13561 gccaacatag gctcgcgtcg ttcgtgtgaa ctgattatca gcgagggccg cgtgacggtg
    13621 ggcggccgtg tggccaggct tggcgacaag gccgatcccg accacgatcg gatcgcggtc
    13681 gatggccggc tgctctttgc cgacaggaat gaggaaggag cgcaaaccgg ccttatctac
    13741 attgccctga acaagccgcg aggggtcatc tcttcgcttg aagatgagtt ggaggtgggc
    13801 cgcaccacag ttcgcgacct catcgagata gagggcgaac accatatcta tcccgttggg
    13861 cgattggaca agccaagtga ggggctggtg ttgatgacga acgatggcgc tctggctcat
    13921 cgtctgactc atcctcgcta tggtcatgag aaggtttacg atgtcacagt ggaaggcgac
    13981 atctctgagg agctgctcga ccaatggcgt catggtgtag ttctggacgg gcgcatcacc
    14041 gcgccggcac ctatcgaagt tgtcgaaaga acccaacagg ctacccggct gcgcattacc
    14101 ttgcgcgagg ggcgcaaacg ccagattcgc cgcattgccg cggcgctggg ccaccccgtg
    14161 cagcggctga ttcgtgagcg gattgggcct ttggcgctgg gtgatttgcc gccgggtcag
    14221 tggcgctatc tgaccgaacg agagatttcc aagctaaagc ggagcgcctt cgatccatcg
    14281 accgccgcgg gttcaacgcc acaaaacaag ccgctccggt acgctaaaca ttcgaacagg
    14341 aaaagggaaa cccgtgcagc ctgattattt gcctgatgtg attgcgattg acggcccggc
    14401 cgcatccggg aagtcgacca ttggtttgat gttggctcaa cgattgggct atttgtgtct
    14461 ggataccgga tcgatgtatc gcgccttgac cttagccgcg atctggcatg gcatcagccc
    14521 cgatgatgaa gaggccattg tgcaattggc gatcgattgc gatctcgaca tattgccgtt
    14581 gtccggagag acggacgggc ggcactatac ggtcttgctg gaaggtgtgg atgtgacctg
    14641 ggatttgcgt ctgccggcgg tggacgcatc cgtgtcccaa atatccacgt atccaggagt
    14701 tcgcgccgag atggtgcggc gacaacggga agtagggcgc agaggccatg tcatcatgat
    14761 cggccgcgat atcggcacgg tcgtcatgcc ggatgctgcc ctcaagctct atatcatcgc
    14821 ctcgcccgag gaaagggcca ggcggcgctt gctcgatcgt cggcgccagg ggcatgatga
    14881 cgattacgaa tcgatcctgg ccgacgtggc tcgccgtgat gccattgata gcggccgtaa
    14941 acattccccc cttcgcccgg cggccgacgc gatccaaatt gacaccaccg gccgcacggc
    15001 cgaagaagtg atagccgaga tcatggccct gatagaccag acccaaccgg aaggacgcga
    15061 ctcataatgg ctcttttcaa cgaaatcgat cccaatacct tttcgggcgg ccaaattatc
    15121 ggcatcgagc cgggtagtgt ggccgcggcg ttaggcctgc gccccggaga cgaactgctg
    15181 gcgatcaatg atcaagcggt cgaggatatt atcgatgtcc agttctatgc cgctgaagag
    15241 gaactggagc tgctcgtgcg tcgcgagggc gaggtcttgc tgtttgaagg tgagcgcaac
    15301 tacaatcaga ccttggggct tgagttcgcg catccgacct tcgatgtgga tattcgccgc
    15361 tgcgacaact tatgtgaatt ctgcttcgtc ctgcagatgg ggccgaaata tcgccgcacg
    15421 ctttacgtca aggatgatga ttaccgctat tccttcctct tcggccatta cgtcacgctg
    15481 acgaatctca acgaccacga ctggtggcga atagaaaaca tgcgcctgtc gccgctctat
    15541 gtatctgtac acgtgactga tacggagacg cgccgtcgtt ttctgcgcaa tccgaacgcc
    15601 ggcgacatca tggaacaatt gcgctggctg gccgaacacc atatcgaggt gcacgcccaa
    15661 ctggtgatcg tccccggctt caatgatggt gaatggctgg cgcagtcggt gaaagacttg
    15721 gctaccctat ggccggatga tgatgtcgtt ggaggcgtcg tgtcggtcag tgttgtgccg
    15781 gtcggtctca cgaaatttca taaatacggg atgcggccgc acacgcgcga agaagccctg
    15841 gctacgctgg agtttgttga atcgttgcag caagaatatc tggatgattt tggaattcgt
    15901 ttcgtctatt tgaccgatga atggtatttg gtgaccgggc gcgatgttcc gccgctggaa
    15961 gattacgacg gtcacgatct gcatgaaaac ggtctgggta tggttcgggc gtttctcgat
    16021 gactggatcg tcgtgagcga cgaaattgcc cacacggagc ctaaacctgc tccacgaaca
    16081 attacgctgg ccactgcggc acttttcgcg cccacgctca ggcagaaggc
//
LOCUS       DAOMQG010000037        15819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1614_length_15819_cov_5.676668, whole genome shotgun sequence.
ACCESSION   DAOMQG010000037 DAOMQG010000000
VERSION     DAOMQG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15819
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_15819_cov_5.676668"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1307)
                     /locus_tag="PLC06_03820"
     CDS             complement(54..1307)
                     /locus_tag="PLC06_03820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRO89604.1"
                     /translation="MSDHTQAPNGESFDAFNLDTRIRAGITDAGYDIPTPIQRQAIPY
                     ILEGRDVLGLAQTGTGKTAAFMLPILQRLTRGPLRRPRALIVAPTRELAEQIHQTVEA
                     LGGRTKVRSATVYGGVGKGPQISAFKRGAEILVACPGRLLDHLGEGNVDLSTIEVLVL
                     DEADRMLDMGFLPDIKRLLRYVPNRQQTMLFSATMPPDIRKLADDILKDPATVQVDIS
                     APAKTVSHALYPVPETLKAKLLHSMLEQTRTSRVLIFTRTKHRAKRLAADLDKRGYRV
                     AALQGNMTQNKRQEAMNGFRGGKYDILVATDIAARGIDVSEISHVVNYDMPDTVDAYT
                     HRIGRTGRAEATGEAVTFAVADDEVMVRDIERVLGKPIERRRMEGFDYGTFAPESQRP
                     APKKSVYRSRSFHGAERGGLPAQRR"
     gene            complement(1545..4067)
                     /locus_tag="PLC06_03825"
     CDS             complement(1545..4067)
                     /locus_tag="PLC06_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO89605.1"
                     /translation="MYVKLNNVDRGNKTADPEWLAGLMLRRDSKLVSRFMAYYRELQQ
                     RPRRWRRLLRRKLALTVAGAALLLALAGGPFAAGSVLAEPIGTNATIAVVNGEVGIND
                     NGQCSLVEALINANATSAGQLYDDCQAGNLSGADTVSLPNNGAFELTAVYDTQFYGTG
                     LPLITSVVTIDGSSSDIYRSNDDGTPDFRILAVDSTGDLTLHDTTISNGSVEENYYGG
                     GGIFSRGALTVEGSTITDNYAYLGGGIDAEQVTIINSVISDNVAYPGDVSSPVGGGIN
                     AETLIVENSTISHNTAYYGGGIYAHEATISGSRIVDNVADGEYGGWGGGILSSVLTLT
                     DSTVMDNTAAGGVGGGVGGGVRMDSGTISGSMISGNRAGTGLDDDNQAYPQSGKGGGV
                     AATGSATIVNSTFSDNEANEGGGLFVLGETTLAHSTLSGNNARTETGGSNGQYQTAGG
                     QGGGILVAAESATCGALTASGNLISGNTASEPGREVLAAQDQCAATVTADAFNVFGHD
                     GDSGTAGFILGASDIIPTVGLSAILSPATDNGGPTPTFALPGDSPALDLAPNASCTAA
                     PVDGVDQRGLPRNANGAGGASANECDAGSFERQAAGVFLISPTRGGSIDGLAFQPSDI
                     LQYDPSTGWSIYFDGSDVGVTRNLADFEVLDNGDILMSFAGSQRIAGVGTFAPQDIAR
                     FVPANGTFQLALRGSDNLLTTSGEKIDALADIGNGRWAISTTGTAAVRLPDGTPLKAQ
                     DEDALGLNPDTGEWSPWFDGTPIPGLKTKDVDALWIDPTTGDVYIGIAGAFNLSGIKG
                     DGRDIVKLTKTPGAPGGYVPSLWWDGSAAGFPTNIDGLEIVP"
     gene            complement(4241..4978)
                     /locus_tag="PLC06_03830"
     CDS             complement(4241..4978)
                     /locus_tag="PLC06_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO89606.1"
                     /translation="MRVIALYNIKGGVGKTAAAVNLAWHSARDGRPTLLWDLDPQGAA
                     SWCLQVPPKVKGGRKALLRGKSPLARYVKPTAYDNLELLPADFSYRNMDLDLSEKKRP
                     EKQLRKLLEPLEAHYHTVFLDCPPGLTLTTESMFAAADTLLVPVIPAALSLRTLALID
                     DFLSRMETARPRVLAFYSMVDRRKTLHRQLTDDRHADGRFLFTTIPYAGDIERMAERR
                     APVAEFAPRSAGAAAFAALWGEVVDVG"
     gene            complement(5041..6042)
                     /locus_tag="PLC06_03835"
     CDS             complement(5041..6042)
                     /locus_tag="PLC06_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO89607.1"
                     /translation="MAKQAQTPTRQPSERPSRSELRNSVVWPVAISAVIWVIGLIIYG
                     IAPGRFDVAVSAIIAVGLVVYLFWYQRRLSITPGERILSLLLAVPAIAGITFGMIRGE
                     ALYAITGVSGSLLLLALQRVVSVPFSYRMARRNFSRGNSELALDLVDKAIDARPTFWE
                     SYQLRSLIYLSAMQFRQAERDALKALELRPDAHPVHNTLGQLYLAGGHYERAADAYSK
                     AIDLSPKHALYHFHQGLALYRLGDVREAAEALAASTRLGLPDVTYELQAYYYLTRALE
                     RNGEIEKAEEVSADMVKFRDGLEILKADLKHQPDFPELAALRADLRDMEKRLAAAVS"
     gene            6138..7457
                     /locus_tag="PLC06_03840"
     CDS             6138..7457
                     /locus_tag="PLC06_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRO89608.1"
                     /translation="MTQPSIPGFRDVPRTGVIYVMHRAGELGFTYDSVDWANLGQGAP
                     ETGPLPGAPARINAVTIDPRRHEYGPITGQIALRTKVADLYNTLYRQGKRSQYTWENV
                     SISGGGRVALARIAAALGNINMGHFLPDYTAYEELLSIFKAFIPIPILLDPDLGYQAP
                     LDMIKREIQGRGLKALLVSNPANPTGQLVQGERLAQWVQLARNYRCSLILDEFYSHYI
                     YNGENGGEREKGGKGEGEKGSAAGTPLPKMVSAAAHVEDVDRDPVIIVDGLTKNWRYP
                     GWRISWTLAPRDVIHAIASAGSFLDGGANNPFQSEVLGLLEPECVYQETAAIQAAFRA
                     KRDYMLDRLYKMGVLVEVEPGGTFYVWANLSQLPPPLNDGLQFFEAGLEEKVITVPGV
                     FFDVNPEKRRTYARYRHYARISFGPEMEIIRRGLDGLERMIERHRQI"
     gene            complement(7500..8735)
                     /locus_tag="PLC06_03845"
     CDS             complement(7500..8735)
                     /locus_tag="PLC06_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRO89609.1"
                     /translation="MAPFPLPLAEIMGHWGQYVVYLLIGIGFGFALEVSGFANAPLLA
                     AQFYFKDMTVLKVMFGAIVTAMVLVFLSSAVGLLDYSLVYVNETYLWPGIVGGLIMGV
                     GFILGGYCPGTSVVGAAVGKIDGIIFVLGVMFGIFAFGETVGFYEEFWYSSYMGRFTI
                     PEWLGLPYGVVVVLVVLMALFMFWGAEQLEKIFGGRDLSKEPKWRIYAGGGLLGLALL
                     TAFIGQPTTEQKWARMEAAEMVRLTNREVQIHPAELAKTMTDDSLRTIILDVRPEAEF
                     NQFHLQEARHAPLAALPDMLDELRDAPANAVVVVVGNDEAAATEAWKFLRAESVRSVY
                     ILEGGLNNWIRLFGDAEFVAANEIVGATDDRPAFIFPAALGSRYAFAAPNETLAQSFE
                     FTPKITLEVKRGPGGGGCG"
     gene            complement(8833..9405)
                     /locus_tag="PLC06_03850"
     CDS             complement(8833..9405)
                     /locus_tag="PLC06_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRO89610.1"
                     /translation="MTTISEAQFNEASEEKTAARRVARPYVHPYAGGILLGLVLFTTF
                     FLTGNGLGASGGMNRLVVWFEDLVAPGHVDRTAYLTSMAGGETNPFDSWVVFVFIGLF
                     VGGVLSGWLFGRTKMTTESGPRTPARVRWLFAFLGGSIMGYGARMARGCTSGQALSGG
                     AVLSVGSWAFMFAVFAGGYAVAYFVRKLWN"
     gene            complement(9450..12266)
                     /locus_tag="PLC06_03855"
     CDS             complement(9450..12266)
                     /locus_tag="PLC06_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRO89611.1"
                     /translation="MVPIKNKNHAGVAGFVKFIKPLGMVALVAAVAVLLFGRVVMAQD
                     GEPQTPRPAASPIHPVFPLLDETGANVLESGAAVSTMTTCGGCHDTAFIAANNFHSDV
                     GLGGYSAPAGVPGGRPWDTGAGLFGRWNPILYRHLSPEGDATIDLTTPAWVRLFGERH
                     AGGGPAVTSRAGGSLVDSGLTVDSTVIDPATGELRPWDWAESGAAELNCFTCHLPGAN
                     NDARVAALAAGRFGDATTATLLGTGIVEEQGDAWRYNPEAFDAAGDLRPEFVTVSDPT
                     AANCGSCHGVVHNEETPLTLDALTMADWNTLTTGQIMSPQRLNASGLNLQDKSTLGRS
                     WDIHMERVLECTDCHYSLNNPIYYRETGGNAPEHLTFDPRRMDLGEYIYRPLHQFAKG
                     QSAQGMLAPGFDGTMRRCESCHTIDNSHNWLPYKERHTQAMACETCHTPRLYAPALES
                     IDWTVLAAGGEPVRAFRGMEGATLGAGTLITGYEPVLLPRTNLDGTTQLAPFNLISSW
                     YWVYGEEERPVPLRYLEAAWFEGDAHAPEVVAAFDADGDGRLSHSELTITTDAQRDLI
                     SGRLSAAGLDSPRIRGEVQPFSINHNTARGEWATRNCRACHGEESRLAAAMTLSDRTP
                     GGQLPTLYEGGPVSWPGAIAADESGALHFAPSTAEAGLYVLGHNAARVVNIIGALMIV
                     GVSLGVIIHGGLRVLAARRRAGRAGESAEAVETHQVYIYDVYERLWHWLQTAAILLLL
                     FTGLIIHKPDLFGIFSFSYVVQVHNILALLLVLNAGLSLFYHLASGEIKQYIPRPRGF
                     FDEAFTQALYYLRGIFRDEPHPHEKTRERKMNPLQQVTYVMVLNILLPAQIITGAFMW
                     AAQGWGAQRWPGAAEALGGLPWLGPLHTLVAWSFAAFIILHVYLTTTGPTPMTGIKAM
                     MMGWEEVEITNYELRITNEEMAAD"
     gene            complement(12364..13797)
                     /locus_tag="PLC06_03860"
     CDS             complement(12364..13797)
                     /locus_tag="PLC06_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HRO89612.1"
                     /translation="MKEFRFIWVVGLFVTILIIAIPVLLFASSGDEASPDPWAYVQPT
                     PAHTDHSALMAGPYADGSAVTVACLQCHEAAASEIMATTHWTWESEPLMLPGRDEPVT
                     VGKKNQINNFCIGIQGNWTGCTQCHTGYGWEDASFDFQEATNVDCLVCHADTGLYGKG
                     TAGNPADGVDLVAAAGSVRRPTRQNCGSCHFDGGGGNAVKHGDLDESLYYPDATVDVH
                     MGRAGFECVDCHAGGNHQITGHMISVSPQVKDELACTNCHSATLHADERINAHTAAVA
                     CQTCHIPEGALRHATKMDWDWSTAGQDLPEDPHTYLKIKGNFVYEHEFRPTYAWWNGA
                     GDRYLLGDRIDPAQTTTLTRPLGDIDDPAAKIWPFKVHTARQPYDVINQYLLVPVTAG
                     EDGFWTNFDWEQAFRLNEENTGLAFSGEYGFAQTEMYWSLTHMVQPKERALQCYDCHG
                     ENGRMDWAALGYFGDPMTWGGRVTNDE"
     gene            complement(13842..14174)
                     /locus_tag="PLC06_03865"
     CDS             complement(13842..14174)
                     /locus_tag="PLC06_03865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO89613.1"
                     /translation="MCVEQPSIDELNLLHAGVCQALGDPKRLLILYALNERPRHVSQL
                     AADLDMPQPTASRHLQILRERHLVLAQRDGAAVVYHLAEPRVIEVLDTMRQILRDSLA
                     RQTGVVNA"
     gene            14413..15033
                     /locus_tag="PLC06_03870"
     CDS             14413..15033
                     /locus_tag="PLC06_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRO89614.1"
                     /translation="MATSPELNVGQRIRSLRERKGLSLRALADRCGLSTTAISQIERG
                     DNSPTVSSLHVLATALGVSITDLFQDERRASAVFVSPGERLRAESNGVLMESLGVGLP
                     YQQLEPFMVTLEPGAGNADQPVSHGGEELVLCLEGAFDYCVDGHPYHLEAGYSLIFEA
                     IRPHYFHNPTDRPVHFLLVFQAGGGGALARRLHVEAGHEFHFGPNE"
     gene            15068..15676
                     /locus_tag="PLC06_03875"
     CDS             15068..15676
                     /locus_tag="PLC06_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HRO89615.1"
                     /translation="MSKGSLLYWPIVAILAIVILATAACGGATEPAAVDVAPTGAADD
                     VPAGVMQAREAVLKFLRQGANECVPPEQAGWTLDDVPNPPDGYNVWRFLSGGCAMTIT
                     TAHEPAAEPIYHVALGDGPTGFCWQAVVDGRGQILLTGSAAQSDPALGNPAQNYCEQQ
                     GYTFEVVTLGSGQMCGMCAFDDDRACNAWAFFHGACTPENAP"
BASE COUNT         2790 a         5119 c         5029 g         2881 t
ORIGIN      
        1 gtaattcgta attcgtaatt cgtaattcgt aattcgtaat tcgtaattcg taattatctt
       61 ctctgcgccg gcagcccacc gcgctccgcg ccgtggaagc tgcgcgagcg atagacgctc
      121 ttcttcggcg cggggcgctg gctttcgggc gcaaacgtgc cgtaatcgaa cccttccatg
      181 cggcggcgct cgatgggctt gcccagcacg cgctcgatgt cgcgcaccat cacctcgtcg
      241 tcggctacgg cgaaggtgac cgcctcgccg gtggcctccg cccggccggt gcggccgatg
      301 cggtgggtgt aggcgtcgac ggtgtcgggc atgtcgtagt tgaccacatg ggagatctcg
      361 ctcacgtcga tgccacgggc ggcgatgtca gtcgccacga ggatgtcgta cttgccgcca
      421 cgaaagccgt tcatggcctc ctggcgcttg ttctgggtca tgttgccctg cagggccgcc
      481 acgcggtagc cgcgcttgtc caggtcggcc gccaggcgct tggcgcggtg cttggtgcgg
      541 gtgaagatga gcacacgcga ggtgcgcgtc tgctccagca tcgaatgcag cagcttggcc
      601 ttcagcgtct ccggcaccgg gtagagcgcg tgggagacgg tcttggccgg ggcgctgatg
      661 tcgacctgca ccgtggccgg gtctttcagg atgtcgtcgg ccagcttgcg gatgtcgggc
      721 ggcatggtgg ccgagaagag catcgtctgc tgccggttgg gcacgtagcg cagcaggcgc
      781 ttgatgtcgg gcaggaagcc catgtcgagc atccggtcgg cctcgtcgag caccagcacc
      841 tcgatggtgc tcagatcgac gttgccctcg ccgagatggt cgagcaggcg gccagggcag
      901 gcgacaagaa tctccgcgcc gcgcttgaag gcactaatct gcgggccttt gcccacgccg
      961 ccgtagacgg tggcgctgcg cactttggtg cggccgccca gagcctcaac cgtctggtga
     1021 atctgctccg ccagctcgcg cgtgggagcg acgatgaggg cgcgcggccg ccgcagcggg
     1081 ccgcgggtca gccgctgcag gatgggcagc ataaaagcgg ccgtcttgcc ggtgccggtc
     1141 tgggccaggc ccaacacatc acgcccttcg aggatgtagg ggatggcctg tcgttgaata
     1201 ggggtgggga tgtcgtatcc ggcgtcggtg atgccggcgc ggatgcgcgt gtccagatta
     1261 aacgcgtcga acgattcgcc gttcggcgct tgggtgtgat cgctcactgg gtatactcct
     1321 tgacttcaga cagccaagtc gcactcccag ccgtgtgtaa aagtctgcaa ttattactct
     1381 acgttctttg gcgaccggtt tcatagccgc gacgccattg gacagtatac aggaacgggg
     1441 ttgttatggc aagtgggcgg gcgggatgct cataatcggc gaattacgaa tgaagagcgc
     1501 agcctcatgg gccacactct tcattcgtaa ttcgtaattc gtaattacgg cacgatctcc
     1561 agcccgtcga tattcgtcgg gaagcccgcg gccgaaccgt cccaccacag tgagggcacg
     1621 tagccacctg gtgcgccggg ggtctttgtc aacttcacga tatcgcggcc gtcgcctttg
     1681 atcccgctga ggttaaacgc cccggcgatg ccgatgtaga catcacccgt cgtcgggtcg
     1741 atccacaggg cgtccacgtc tttggtcttc agaccgggga tgggcgtgcc gtcgaaccag
     1801 ggcgaccact caccggtgtc gggattcagt cccagcgcgt cttcgtcttg cgctttcagg
     1861 ggggtgccgt cgggcagtcg aaccgcggcc gtcccggtgg tgctgatcgc ccagcggccg
     1921 ttgccgatgt cggccagagc gtcgatcttt tcgccggaag tcgtcagcaa attgtccgag
     1981 ccgcgcagcg ccaactggaa cgtcccgttc gcgggcacaa agcgggcgat gtcctgtggg
     2041 gcgaaggtgc ccaccccggc gatccgctgg ctgccggcga acgacatcag gatgtcgccg
     2101 ttatcgagca cctcgaagtc cgccaggttc ctggtgacac ccacgtccga gccgtcaaag
     2161 tagatcgacc agccggttga gggatcgtac tgcagaatat ccgacggctg gaaggccagg
     2221 ccgtcgatcg aaccgcccct ggttggagag ataaggaata ctcctgcggc ctggcgctcg
     2281 aaactgccgg cgtcacattc gttggccgac gcgccgcccg cgccgttggc gttgcgcggc
     2341 agtccgcgct ggtcgacgcc atccaccggc gcagccgtgc atgaggcgtt gggggccagg
     2401 tcgagcgccg ggctgtcgcc gggcaaggcg aaagtcggcg ttgggccgcc attatcggtc
     2461 gccgggctga ggatggcgct caggccgacc gtcgggatga tgtcgctcgc gccgaggatg
     2521 aagccggccg tgccgctatc gccatcgtgg ccgaagacgt tgaacgcgtc ggcggtgacg
     2581 gtcgcggcgc attgatcctg cgccgccagc acctcgcgcc ccggttcgga cgccgtattg
     2641 ccggaaatga ggtttccgct cgccgtcaac gcgccgcagg tggccgattc ggcggcgacc
     2701 aaaatcccgc cgccctgccc accggcggtc tgatattggc cgttcgagcc gccggtttcg
     2761 gtacgggcgt tgttgccgga gagggtgcta tgagccaggg ttgtttcccc caggacgaac
     2821 aggccgccac cctcattggc ctcgttatcg ctgaacgtgc tgttgacgat tgtggccgat
     2881 ccggttgcgg cgacgccgcc gcccttgccg gattggggat aggcctggtt atcgtcatcc
     2941 agcccggtgc ccgcccggtt gccgctgatc atcgagccgc tgatcgtgcc gctgtccatg
     3001 cggacgccgc cacccacgcc gccacccacg ccgccggcgg cggtattgtc cataacggtg
     3061 ctgtcagtca gcgtcagcac tgatgacagg atgcccccgc cccagccgcc gtactcacca
     3121 tcggccacgt tgtcgacaat ccggctgccg gagatggtcg cctcgtgtgc gtagataccg
     3181 ccgccgtaat aagccgtatt gtgggagatg gtgctgttct caacgataag ggtctctgcg
     3241 ttgatgccgc cgcccacggg agaagacacg tcgccgggat acgccacatt atcgctgatg
     3301 acactgttga tgatagtcac ctgttcggcg tcgatccccc cgcccagata ggcgtaatta
     3361 tcggtgatcg tgctgccttc caccgtcagc gcgcctcggc tgaagatgcc gccgccaccg
     3421 taatagtttt cttccacaga gccattactg atcgtcgtgt cgtgcagcgt cagatcgccg
     3481 gtcgaatcga cagcaaggat gcggaagtcc ggcgttccat catcattact tcgataaatg
     3541 tcggaactac tgccgtcgat agtcaccacg gatgtaataa gcggcaaccc tgtcccataa
     3601 aattgggtgt catagacggc agtcagctcg aatgcgccgt tgttcggtag agacacggtg
     3661 tccgccccgc tcagattgcc cgcctgacaa tcgtcgtaga gttgcccggc cgaggtggcg
     3721 ttggcgttga tgagagcttc caccagcgag cactggccgt tgtcgttgat accgacctcg
     3781 ccattgacca ccgcgatcgt cgcgttggtg ccgataggct cggcgaggac gctgcccgcc
     3841 gcgaacggcc cgccggccag cgccagcagc agcgccgcgc cggcgacggt gagcgccagt
     3901 ttgcgtcgca acaaccgtcg ccagcggcgc ggccgctgct gcaattcccg ataataggcc
     3961 atgaaacgcg acacgagctt gctgtcgcgc cgcaacatca gaccggccag ccactcaggg
     4021 tcggccgtct tgttgccccg atctacatta ttcagtttga cgtacatgat ggactgcctt
     4081 gtcagttgat ggcccggcgt gggtgttgat tcgactggaa atccaatcgt gtggtgccgg
     4141 atttcatgac tggcattcat gaacatagtt ccggaagatt acgaaaaaga ttaagatttt
     4201 cggcgggatc aggaagcttt aagaattgaa tcgcgggggt ttacccaaca tccaccacct
     4261 ctccccacaa cgccgcaaac gccgccgccc cggccgaccg gggcgcgaac tcggccaccg
     4321 gcgcgcggcg ttcggccatg cgctcgatgt cgccggcgta ggggatggtc gtgaagagga
     4381 agcggccgtc ggcgtggcgg tcgtcggtca actggcggtg cagcgtcttg cggcggtcga
     4441 ccattgagta gaaggccagc acccgcggcc gggccgtctc cattcgcgac agaaagtcgt
     4501 cgatgagcgc cagggtgcgc aaggacagcg ccgcggggat gaccggcacc agcagcgtgt
     4561 cggccgcggc gaacatgctc tccgtcgtca gcgtcagccc cggcgggcag tcgagaaaga
     4621 cggtgtggta gtgggcttcc agcggctcca gcagcttgcg cagctgcttc tccggccgct
     4681 tcttctcgct caagtcaagg tccatgttgc gataggagaa atcggccggc agcagttcca
     4741 gattatcgta agccgtcggc ttcacgtatc gggccagggg gcttttgccg cgcagcaacg
     4801 ccttgcggcc gcctttcacc ttcggtggca cttgcaggca ccaggacgcc gcgccctgtg
     4861 ggtcgagatc ccacagcagc gtcgggcggc cgtcgcgcgc cgagtgccag gccagattga
     4921 ccgccgcggc cgtcttgcca accccgccct tgatgttata caaagcgatg acgcgcacgg
     4981 gctaattatg acccaagcgg ggcgagtgac aaattaagga gtcgaaaggg aaggcgacca
     5041 tcaggaaaca gccgcggcca gccgcttctc catgtcgcgc aggtcggcgc gcagggcggc
     5101 cagttcgggg aaatcgggct gatgtttgag atcggccttc aggatttcga ggccgtcccg
     5161 gaacttcacc atgtcggcgg agacttcttc cgccttctcg atctcgccgt ttcgctccag
     5221 cgcgcgggtg aggtagtagt aggcctgcag ctcataggtg acgtcgggca gacccagccg
     5281 cgttgacgcc gccagcgctt cggccgcctc gcgcacgtcg cccagccggt agagggccag
     5341 cccctgatgg aagtgataga gggcgtgctt gggtgacagg tcgatggcct tgctgtaggc
     5401 atcggccgcg cgctcgtaat gccccccggc cagatagagc tggccgagcg tgttgtgaac
     5461 cgggtgagcg tcgggccgca gctccagcgc cttcagcgcg tcgcgctcag cctggcggaa
     5521 ctgcatcgcc gacaggtaga tgagcgaccg cagctggtag gactcccaga acgtcgggcg
     5581 agcgtcgatg gctttgtcca ccagatcgag cgccagttcg gagttgccgc ggctgaagtt
     5641 gcgccgggcc atgcgatagg aaaacggcac cgacacgacg cgctggagag ccagcagcag
     5701 cagactgccg ctgacgccgg tgatggcgta gagcgcctcg ccgcggatca tgccgaaggt
     5761 gatgcccgcg atggccggca cggccagcaa cagcgagagg atgcgctcgc cgggcgtgat
     5821 ggacagccgc cgctgatacc agaacaaata gacaaccagc cccacggcga tgatagccga
     5881 cacggccacg tcgaaccggc cgggagcgat gccatagatg atcaggccga tcacccagat
     5941 gacggccgag atggcgaccg gccacacgac gctgttgcgg agttcgctgc gcgacggccg
     6001 ttcgctgggc tgtcgggtgg gtgtctgtgc ttgtttggcc acggctacct cattgacatt
     6061 cgcgcgggtt cacatgacaa tacgggtttg cttggcgggc aatgctcgct catcacgatc
     6121 ataaggcaat caattcgatg acgcaaccct caattccggg atttcgcgac gtgccgcgca
     6181 ccggcgtaat ctacgtcatg caccgcgccg gcgaactcgg cttcacctac gattcggtcg
     6241 attgggccaa tttgggccag ggcgcgccgg agaccggccc cctgcccggc gcgccggcgc
     6301 gcatcaacgc cgtcaccatc gacccgcgcc gccatgaata cggccccatc accggccaga
     6361 tcgcgctgcg gacgaaggtg gccgacctct acaacacgct ctaccgtcag ggcaagcgca
     6421 gccagtatac gtgggagaac gtcagcatct ccggcggcgg ccgcgtggcc ctggcccgca
     6481 ttgccgccgc gctgggtaac atcaacatgg gccacttctt gcccgactac accgcctacg
     6541 aggagctgct gagcatcttc aaggcgttca tccccatccc catcctgctc gaccccgacc
     6601 tgggctatca ggccccgctg gacatgatca agcgcgagat tcaggggcgc gggctgaagg
     6661 cgctgctcgt gagcaacccg gccaatccga ccgggcaact cgtccagggc gagcggctgg
     6721 cccaatgggt gcagctggcc cgcaactatc gctgctcgct catcctcgac gagttctatt
     6781 cccactacat ctataatgga gagaacgggg gagaaaggga gaaaggagga aagggagagg
     6841 gggagaaagg gagcgccgcc gggactccgc tgcccaaaat ggtctcggcc gcggcccacg
     6901 tcgaggatgt cgaccgcgac ccggtcatca ttgtcgacgg cctcaccaag aactggcgct
     6961 atcccggctg gcgcatcagc tggacgctgg cccccaggga cgtcatccac gccatcgcca
     7021 gcgccgggtc gtttctcgac ggcggggcca acaacccgtt ccagagcgaa gtgctggggc
     7081 tgctggagcc ggagtgcgtc tatcaggaga cggccgccat ccaggccgcc ttccgcgcca
     7141 agcgggacta catgctcgac cggctctaca agatgggcgt gctggtggaa gtggaaccgg
     7201 gcggcacctt ctacgtctgg gccaacctgt cgcagctgcc gccgccgctc aacgacggcc
     7261 tacagttctt cgaggccggg ctggaggaga aggtcatcac cgtgccgggg gtgttcttcg
     7321 acgtgaaccc cgagaagcgc cgcacctacg cccgctatcg ccactacgcc cgcatcagct
     7381 tcgggccgga gatggagatc atccgccgcg gcctcgatgg gctggagcgg atgattgagc
     7441 ggcacaggca gatctgacag gttttcggca aacctgtcag atcacctctc tgaactctct
     7501 tatccgcagc ctcccccacc cgggccgcgc ttcacttcca gcgtgatctt cggcgtgaac
     7561 tcaaacgact gcgccagcgt ctcgttgggc gcggcgaagg cgtaccggga gccgagcgcc
     7621 gccggaaaga tgaacgccgg tcgatcgtcg gtcgcgccga cgatctcgtt tgcggccaca
     7681 aattcggcgt cgccgaacag acgaatccag ttattcagcc cgccttccaa tatatagacg
     7741 ctgcgcacgc tctcggcccg caggaacttc cacgcctcgg tcgcggccgc ttcgtcgttg
     7801 cccaccacca ccaccacggc gttcgccggc gcgtcgcgca gctcgtccag catgtcgggc
     7861 agcgccgcca agggcgcgtg acgcgcttcc tgcaagtgga actggttgaa ctccgcctcc
     7921 ggccgcacgt cgaggatgat cgtccgcagc gagtcgtcgg tcatcgtttt agccagctcg
     7981 gcggggtgga tctgcacctc gcggttggtt agccggacca tctcggccgc ttccatccgt
     8041 gcccacttct gttcggtcgt gggctggccg atgaaagccg tcagcagtgc caggcccagc
     8101 agcccgcccc cggcatagat gcgccatttc ggctccttgc tcaggtcgcg gccgccgaaa
     8161 atcttctcca gttgttccgc gccccagaac atgaacagcg ccatcagcac gaccagaacg
     8221 accaccacgc catagggcag ccccagccat tccggtatgg tgaaccgccc catgtaggag
     8281 gagtaccaga actcctcata gaagccgacc gtctcgccga aggcgaagat gccgaacatg
     8341 acgcccagga cgaagatgat gccgtcgatc ttgcccacgg ccgcgccaac caccgacgtg
     8401 ccggggcaat agccgcccag gatgaagccg acgcccatga tcagcccgcc gacgatgccc
     8461 ggccacagat aggtctcgtt gacatagacc aggctatagt cgagcaagcc gacggccgaa
     8521 ctcaggaaca ccagcaccat cgccgtgacg atggctccga acatcacctt gagcacggtc
     8581 atatccttga aatagaactg cgcggccaat agcggcgcat tggcgaagcc cgacacctcc
     8641 agcgcaaagc cgaagccgat gccgatcagc aaatagacga cgtactgacc ccaatgcccc
     8701 ataatctcgg cgagtggtaa tggaaacggc gccatgatca ttctcccgaa agtttatcgg
     8761 aagggttatc cgcagatttc gcagattagc caatctgtga aatctgtgaa atctgtggat
     8821 tccctcttct tcctaattcc aaagtttacg gacaaaatac gccaccgcat agcccccggc
     8881 gaagacggcg aacatgaacg cccaactgcc gaccgacagc accgcgccgc cggacagcgc
     8941 ctggccgctg gtgcagccgc gggccatgcg cgccccgtag cccatgatcg acccgccgag
     9001 gaaagcgaac agccagcgca cccgcgccgg cgtgcgcggg ccgctctcgg tggtcatctt
     9061 cgtgcggccg aacagccacc ccgacagcac accgcccacg aacaggccga tgaacacaaa
     9121 gaccacccag ctgtcgaacg ggttggtctc gccgccggcc atgctcgtca gataggccgt
     9181 tcggtccacg tggccggggg cgacgaggtc ctcgaaccag acgacaagcc ggttcatgcc
     9241 gcccgatgcg cccagaccgt tgccggtcag gaagaacgtc gtaaacagga caagacccag
     9301 caggatgccg cccgcatagg gatggacgta gggccgggcc acgcgccgcg cggcggtctt
     9361 ctcctcactc gcttcattga attgtgcttc cgaaatcgtt gtcatcgctc tctccgtggt
     9421 tgccacgtgg tggccccgtg gtggccgcct caatccgccg ccatttcttc attcgtaatt
     9481 cgtaattcgt aattcgtaat ttcaacctct tcccacccca tcatcatggc cttgatcccg
     9541 gtcatgggcg ttgggccggt cgtggtcaga tagacgtgga gaatgatgaa cgccgcgaag
     9601 ctccacgcca ccagcgtgtg cagtggcccc agccacggca gcccgcccag cgcctcggcc
     9661 gcgcccggcc aacgctgcgc cccccagccc tgggctgccc acatgaatgc gccggtgatg
     9721 atctgcgcgg ggagcaggat gttgagcacc atgacgtagg tcacctgctg cagcgggttc
     9781 atcttgcgct cgcgcgtctt ttcgtgggga tgcggctcgt cgcggaagat gccgcgcagg
     9841 taatagagcg cctgcgtgaa cgcctcgtcg aagaagccgc gcggtcgggg gatatactgc
     9901 ttgatctcgc cgctggccag atgatagaac agcgacaacc cggcgttgag caccagcagc
     9961 agggccagaa tgttatgcac ctggaccaca tagctgaagc tgaagatgcc gaacaggtcg
    10021 ggcttgtgga tgatcagtcc cgtgaagagc agcaacagga tggccgccgt ctgcagccag
    10081 tgccacagcc gctcatacac gtcgtagatg tagacctgat gcgtctccac cgcctcggcc
    10141 gattcgcccg cccgcccggc tcgtctccgc gccgccagca cccgcagccc gccgtggatg
    10201 atgacgccca gcgaaacgcc aacgatcatc agcgcgccga taatattgac gacgcgcgcg
    10261 gcgttgtgac ccaatacata cagccccgcc tcggccgtgc tgggcgcgaa atgaagcgcg
    10321 ccgctctcgt cggcggcgat cgcgcccggc caactcaccg gcccgccctc gtagagcgtc
    10381 ggcagttggc cgccgggcgt gcggtcggac aaggtcatgg ccgccgccag ccgcgactct
    10441 tcgccgtggc aggcgcgaca attgcgcgtc gcccactcgc cgcgagcggt gttgtggttg
    10501 atgctgaacg gctgcacctc gccgcgaatg cgcggggagt ccagcccggc ggccgacaac
    10561 cggccgctga tgaggtcgcg ctgggcgtca gttgtgatag tcaattcgct atgcgaaagc
    10621 cggccgtcgc catctgcgtc aaacgcggcc acgacttccg gcgcgtgggc atcgccctcg
    10681 aaccaggctg cctccagata gcgcagcggc accggccgct cttcctcgcc atagacccag
    10741 taccagctgc tgatcagatt gaacggggcc aactgggtcg tgccgtcgag attcgtccgc
    10801 ggcagcagca ccggctcgta gccggtgatc aaggttcccg cgcccaacgt cgccccctcc
    10861 atgccgcgaa aggcgcgtac cggttcgccg ccggccgcca gcaccgtcca gtcgatcgac
    10921 tccagcgccg gggcatagag ccggggcgtg tggcatgtct cgcaagccat cgcctgggta
    10981 tggcgctcct tgtagggcag ccagttgtgg ctgttgtcga tggtgtggca cgactcgcag
    11041 cggcgcatcg tcccgtcgaa gccgggggcc agcatgccct gggcgctctg ccccttggcg
    11101 aactggtgca gcggccgata gatgtactcg cccaggtcca tgcgtctcgg gtcgaaggtc
    11161 aggtgttcgg gcgcgttgcc gcccgtctcg cggtagtaga tcgggttgtt gagcgagtag
    11221 tggcagtcgg tgcattccag cacgcgctcc atgtggatgt cccacgagcg gccgagcgtg
    11281 ctcttgtcct gcaagttcag cccactcgcg ttgaggcgct gcggagacat gatctggccg
    11341 gtggtcaatg tgttccagtc ggccatcgtc agcgcgtcga gcgtcagggg cgtctcctcg
    11401 ttgtggacca cgccgtggca gctgccgcag ttggccgccg tcgggtcact gacggtcaca
    11461 aactccggcc gtaaatcgcc cgccgcgtcg aacgcctcgg ggttatagcg ccatgcatcc
    11521 ccctgctcct caacgatgcc cgtccccagc agcgtcgccg tcgtcgcgtc gccgaagcgg
    11581 ccggcagcca gtgctgccac gcgggcgtcg ttgttggccc ccggcagatg gcaggtgaag
    11641 cagttcaact cggccgcgcc cgattccgcc cagtcccacg gccgcagttc gcctgtggcc
    11701 ggatcgatga ccgttgaatc gaccgtcagc ccggaatcga cgagcgaacc gcccgcgcga
    11761 ctggtgacgg ccgggccgcc gcccgcgtgg cgttcgccga acagtcgcac ccaggcgggg
    11821 gtcgtcaggt cgatcgtcgc gtccccttcg ggcgagagat ggcgatagag gatggggttc
    11881 catctgccga acaggcccgc gccggtgtcc cacggccgcc caccggggac gccggccggc
    11941 gcgctgtagc cacccagccc gacgtcgctg tggaagttgt tggcggcgat gaaggccgta
    12001 tcatggcagc cgccgcaggt ggtcatcgtg gagacggccg cgccgctctc cagcacgttg
    12061 gccccggttt catccagtag cgggaaaacg gggtggatgg gcgaggcggc cgggcgcggc
    12121 gtttgcggct cgccgtcctg cgccatgacg acgcggccga acagcaacac cgccactgcc
    12181 gccaccagcg ccaccatccc caaaggcttg atgaatttca cgaagccggc cacgccggcg
    12241 tggttcttgt ttttgatggg taccataaac cacctgggtt aagtacgaag agcgcgcgct
    12301 tcattcgtca ttcgtcactc gtcactcgtc actcgtcact cgtcattcgt cactcgtcac
    12361 tcgtcactcg tcattcgtca ctcgtccgcc ccaggtcatg gggtcgccga agtagccgag
    12421 cgcggcccag tccatgcggc cgttctcgcc atggcagtcg tagcattgca gtgcccgctc
    12481 cttcggctgc accatgtggg tcaatgacca gtacatctcc gtttgggcga agccatattc
    12541 gccactgaag gccaggccgg tgttctcctc gttcagccgg aaggcctgct cccagtcaaa
    12601 attggtccag aagccgtcct ctccagccgt caccggcacc agcagatatt gattgatgac
    12661 gtcatacggc tgccgcgcgg tgtggacctt gaacggccat atcttcgccg ccgggtcatc
    12721 gatgtcgccc aacggccgcg tgagggtcgt ggtctgcgcc gggtcgatgc gatcgcccag
    12781 cagatagcgg tcgccggcgc cgttccacca ggcgtaggtg gggcgaaact cgtgctcata
    12841 gacgaagttg cccttgattt tgagataggt gtgcgggtct tcgggcaggt cttgcccggc
    12901 ggtcgaccag tcccaatcca tcttggtggc gtgccgcagc gccccttcgg ggatgtggca
    12961 agtctggcag gccacggccg cggtgtgggc gttgatgcgc tcgtcggcgt ggagcgtggc
    13021 gctgtggcag ttggtgcagg ccagctcgtc cttcacctgc gggctgacgg agatcatgtg
    13081 gccggtgatc tgatggttcc ccccggcgtg gcaatcgacg cactcgaacc cggcgcggcc
    13141 catgtggaca tcgaccgtcg catcgggata atagaggctc tcgtccaggt cgccgtgttt
    13201 caccgcattg ccgccgccgc cgtcgaaatg gcaactgccg cagttctgac gcgtcggccg
    13261 ccgcacgctg cccgctgccg cgaccagatc aacaccatcg gccgggttgc ccgccgtccc
    13321 cttgccatat agccccgtgt cggcatggca caccaggcaa tcgacgttcg tcgcttcctg
    13381 gaagtcgaac gaggcgtcct cccagccgta accggtgtgg cattgggtgc agccggtcca
    13441 gttgccctga ataccgatgc aaaagttgtt gatctggttc ttcttgccga cagtcaccgg
    13501 ctcgtcgcgg ccgggcagca tcaacggctc gctctcccac gtccagtggg tcgtggccat
    13561 gatttcgctc gcggccgcct cgtggcattg cagacaggcg acagtgacgg ccgaaccgtc
    13621 ggcatatggc ccggccatca gcgcactatg gtcggtgtgg gccggcgtcg gctgaacgta
    13681 ggcccatgga tcaggcgacg cctcgtcccc cgacgaggcg aagagaagga ccgggatcgc
    13741 aatgatcagg atcgttacaa acagaccgac gacccagata aaccgaaact ccttcatagt
    13801 ggctccattc cagaggtgac aggctcatgg ccctgtatat atcaggcgtt gacaactccc
    13861 gtctggcggg cgagtgaatc gcgcaggatt tggcgcatcg tgtccagcac ctcgatgacg
    13921 cgcggctcgg cgagatgata aacgaccgcc gccccgtcgc gttgggccag caccagatgg
    13981 cgttcgcgca agatttgcag atggcgggag gcggtcggct gaggcatatc gagatcggcg
    14041 gccaattggc tcacatgacg cggccgttcg ttgagggcat agaggatgag cagccgtttc
    14101 gggtcgccca gcgcctggca gacgccggca tgcaacaggt tcagttcatc gatcgacggc
    14161 tgttccacgc acatagccct catgtattcg gatagatgaa tacataggta ctttacgcga
    14221 gagccggtgg ctgctccagt gacattgatc acacgtgggg catgacaagt gtcatgtatg
    14281 tcgcagtgcg tcatgacctg atcggccgtg ccgccagaca attgctaata tcaatcggat
    14341 gacaactatt gtttacaaat atcaattatt attgtatact tggttcatat tccgtttcga
    14401 tggagaggct gcatggcgac aagccctgaa ctcaatgtgg gccagcgtat ccgctcgttg
    14461 cgcgagcgaa agggtctctc gttgcgcgcg ctggcagatc ggtgcggcct ctccaccacc
    14521 gccatcagcc agattgagcg cggcgacaat tcaccaaccg tctcctcgct ccatgtgctg
    14581 gcgacggcgc tgggcgtctc gatcactgac ctgttccagg acgagcgccg cgcgtcggcc
    14641 gtctttgtgt cgccgggcga gcggcttcgc gccgaaagca acggcgtgct gatggaaagc
    14701 ctgggcgtcg gtctgcccta ccagcagctg gagccgttta tggtgacgct ggagccgggc
    14761 gcgggcaacg ctgaccagcc tgtttcccat ggtggcgagg agttggtgct atgtctggaa
    14821 ggggcgtttg attattgcgt cgacggccac ccctatcatc tcgaagcggg gtatagcctg
    14881 atcttcgaag caattcggcc gcactacttc cacaatccga ccgaccgacc ggtccacttt
    14941 ctgctggtct tccaggccgg tggcgggggc gcgctggcgc ggcggctcca cgtcgaagcc
    15001 ggccatgagt tccatttcgg gccgaacgag taattccggc cggtccgtcg cgtcagggaa
    15061 ggtatccatg agcaagggga gtctgctgta ttggcccata gtggccattc tcgcgattgt
    15121 tattctggcg acggccgcct gtggtggggc cactgaaccg gcggcggtcg acgttgcccc
    15181 aaccggcgcg gccgacgacg tgcccgccgg cgtgatgcag gcccgcgaag ccgtgctcaa
    15241 attcctgcgc cagggagcca acgaatgtgt cccgccggag caggccggtt ggacgctcga
    15301 cgacgttccc aacccgccgg acgggtataa cgtgtggcgc ttcctgtccg gcggctgtgc
    15361 catgacgatc accaccgccc atgaaccggc tgccgagcca atctatcacg tggccctggg
    15421 cgacggcccg accggctttt gctggcaggc ggtggtggac ggtcgcggcc aaatcctgct
    15481 gaccggcagc gccgcccaga gcgacccggc attgggcaac ccggcccaaa attactgcga
    15541 acagcaaggg tacacgttcg aggtggtcac actcgggtca gggcagatgt gcggcatgtg
    15601 cgcctttgac gacgatcgcg cctgcaacgc ctgggccttt ttccatggcg cgtgtacgcc
    15661 cgaaaatgcg ccataagggg ggctggcccc caccgatccg accgatcatc cgcatcctgt
    15721 cgcgcctgcc tgtcaccacc ggtcaggcct caggccccat atcagttact tctacctatt
    15781 gacaatcctt cctgtttaat tttatactaa tacgtatta
//
LOCUS       DAOMQG010000038        15512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1659_length_15512_cov_11.264411, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000038 DAOMQG010000000
VERSION     DAOMQG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15512
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_15512_cov_11.264411"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2068)
                     /locus_tag="PLC06_03880"
     CDS             complement(197..2068)
                     /locus_tag="PLC06_03880"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HRO89616.1"
                     /translation="MNRLEFKERVNNGPILMDGAFGTVLHGRGIPIDQSFDMVNLDNP
                     AVVAEIHRSYIDAGSDLIETNTFGANRFKLSEYGKQDKVVEINHAAVAVARRVIAGSF
                     KEVLLAGSVGPLGARLAPLGRVSSAEAQEAFTEQIDALINAEPQGVDLIIIETMSDIK
                     EIEAAVAAARSISKDIPIIVQMTFTRDDKTLLGYPPEAIATNLTSLDVDAVGINCSSG
                     PAQVLRLAGIVRKLAPDIPVSASPNAGWPEQMQGGRVMYPATPDYFADYARAFLKAGV
                     NLIGGCCGTTSQHIKAMREAIDNPGEFSMSLPEIRITSRPETTVVRGDEPTRLAQHLA
                     GDQLILTVEMDPPKGAAPERLLAGARMLREAGATFLNIADSPLARMRMSAWAAAYLIQ
                     KDVGLETVLHFPTRGRNILRIQGDLLAAHAMGIRNLLVTMGDPMRIGDFPEAMDTYDI
                     VPTGLIELVKKQFNHGLDKAGQSIDQPTTFVVGCALSLTPKNVESELKLLRKKVEHGA
                     DFALTQPVFDPVAAVSFLETYERTYGEAPPPIIAGIKPLYNSRNAEFLHNEVPGIYIP
                     KEKRERMRAADKPVTEGVQIAREIAETLRPYVRGFYIMPAFGRFDLVADVLDSFTKI"
     gene            complement(2191..3246)
                     /locus_tag="PLC06_03885"
     CDS             complement(2191..3246)
                     /locus_tag="PLC06_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRO89617.1"
                     /translation="MNRQPLLPGWLSALLSVAFVLSAIAVLIFAYLTVQHLMTAPDSD
                     ETAAEGIDGGLTSVDGGLAPVTIIEGATAEPAVQPTIGAWEGNDRVNILLVGIDRRPG
                     EAFVSRTDSMMVISVDPEADSMSILSIPRDLYVQIPGYGQDRINTALVYGAREGDYLD
                     GAALAMQTVSSNLGIPINHFVMVDFGAFVRIVDLLEGIDVQVPYDINDSLYPDMDYGY
                     DPLFIPAGMQHFDGQMALKYARTRHADSDFNRAYRQQQVLFAARSKALSLGIGQMILR
                     APSLYREVEGGIRTDLNLEQLVRLAKTVSDIPSDNTQNRVLDQDYVTSFRTPGGASVL
                     LLNSDVAMPMIQELFGN"
     gene            3606..>3910
                     /locus_tag="PLC06_03890"
     CDS             3606..>3910
                     /locus_tag="PLC06_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRO89618.1"
                     /translation="MTFRPRSELMQDDEVIRLVRLFADLGFHKFRLTGGEPTVRANIV
                     EIVRQIAATPGVEKVAMTTNGLLLDRLAQPLVDAGLNRVNISIDTLDPAKFKKLTRWG
                     "
     assembly_gap    3911..4010
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4011..4645
                     /locus_tag="PLC06_03895"
     CDS             <4011..4645
                     /locus_tag="PLC06_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRO89619.1"
                     /translation="ISIDTLDPAKFKKLTRWGNVEDVWRGIRAAEATDLEIKLNAVVV
                     RNYNDQEDVVDLARLTLYRPWQVRFIEMMPFGEISDFQLAGVVTQEEIIKTIESGLGA
                     LTPVNPGELDGEASLYYLENAKGRVGFISSVSHPFCASCNRTRLTADGRLRLCLLREK
                     EVDLLTPMRDGATDTEMLAIIKEGIWWKPWGHQLAEDIVPLNRVMSEIGG"
     gene            complement(4655..5662)
                     /locus_tag="PLC06_03900"
     CDS             complement(4655..5662)
                     /locus_tag="PLC06_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89620.1"
                     /translation="MENRKDLISVVAPVYNEESVLDALYERVRDVLDGAGESWELILV
                     NDGSRDDSAGVIARLHERDPRVKGLSFSRNFGFQIAATAGLDFSQGDAVILTDADLQD
                     PPEVYPAMLAQWRAGYDVVYGVRASRQGETWFKLTTAKLFYRLIHRITSINIPLDTGD
                     FRLMDRRVINSIRGMNERNRFLRGMVPWVGFRQIGVEYNREARYAGEAKFTSVKKMLP
                     FALDAITSFSYFPLQLATILGFIIAGISVLGILGVVTVRLLGPHEPLLGQATTLVVVL
                     FLGSVQLISLGIIGEYLGRIYDEVKGRPLYLIQESWGLTQAPLGRFAPPMGPNSVTNG
                     R"
     gene            complement(5670..6452)
                     /locus_tag="PLC06_03905"
     CDS             complement(5670..6452)
                     /locus_tag="PLC06_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRO89621.1"
                     /translation="MYGFTVATLNLRGRTNRWLARRHLLVGQLLDQLPDIIALQEIDL
                     SIGQGSWLARQVNIRTSGDGRRPYRIVQGRRSGLRNWSRGMGIMTRLPIIYHDTKELG
                     VDGRIALRANLELPAGAAGSRRQSLDFVNVQLHHGAGAFEARAEQAMNLVGWINEKKR
                     VPLQVICGDFNESSQGIAVTFMYQSYRSAFAEVHGRDPLATYPSNLVQPQPVAAECLD
                     YVFLSPAVYKVTGAAIFGDKSAPDDDTLYPSEHVGLLIGLEV"
     gene            complement(6470..7714)
                     /locus_tag="PLC06_03910"
     CDS             complement(6470..7714)
                     /locus_tag="PLC06_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89622.1"
                     /translation="MGRSALIANAAGYIPGSFNLIEFLDNFYAGQHDNGFICRELDPE
                     TGEDFHESYDPNSTGPNTMGWTEWRHFRLTGDKERIAAVFYPLMAYHRWCRANRTWPN
                     GLYWTTAYASSLVNQPRVPDGQYHHQHWAWIDINAQAVLDCTMLERMAVLLGEEELAE
                     EVSSERERLVKTINENMWNGALNFYQDIGPDRSFSPVKSIASYWALLDPHLVPKDRLP
                     LFIQHLRDTWSFRTENVIPSLSADSEAYNARTGNGWRGAVRPALTYMVLRGLGVADQH
                     RLGHKLALVHLDMVDKVFEMTGKFWESYLPEELGPAEPQVEDISGITPASIIPMILEQ
                     VLGLAIDWPLRQVTWRRILTTDEAYGVRNLSLGNDGSLDLIGSNDSIRVRADVPFTLT
                     VHIDKEIVQAAVPAGEFDLSIK"
     assembly_gap    7999..8098
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8194..8646)
                     /locus_tag="PLC06_03915"
     CDS             complement(8194..8646)
                     /locus_tag="PLC06_03915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRO89623.1"
                     /translation="MGSGDTFKIELLPKSAPQTVNSFVFLARNGWFDNITFHRVIPGF
                     VAQTGDPSGTGRGGPGYYIPNEIDSSLSHDEKGVVAMANAGPNTNGSQWYITYGDVSQ
                     LDGSYTIFGKVIEGMDVVERITPRDPSRNPYAPAGDTIARITIEEILP"
     gene            complement(8773..9168)
                     /locus_tag="PLC06_03920"
                     /pseudo
     CDS             complement(8773..9168)
                     /locus_tag="PLC06_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(9187..9465)
                     /locus_tag="PLC06_03925"
     CDS             complement(9187..9465)
                     /locus_tag="PLC06_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89624.1"
                     /translation="MKKIGLLLILLSLFIAACGSGGAEVAEQEGSEEQAAVTESSPTG
                     EPAEGSGVEASDTAISSIDDPLQARERDWKQGNTSNPAVTIIEYGDFQ"
     gene            complement(9553..11796)
                     /locus_tag="PLC06_03930"
     CDS             complement(9553..11796)
                     /locus_tag="PLC06_03930"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRO89625.1"
                     /translation="MGSESVFSAMVGRHNMTIKTGKLAEQAGGAITLQVGESMLLATA
                     TMSKNVREGLDFFPLSVDFEEKMYAAGRIPGGFFRREGKPTTEAVLTSRLTDRPLRPL
                     FPDGMRNEVQVIVTTLSSDSVHHLDIMSVNAASAALTISDVPWNGPVGAVRVGYIDGQ
                     FVADPTIQEMAESNLDLRIAGSRDAIIMVEAAANEVPEAVMVEALAFGHQALQPIITL
                     LEEMQAAVGKEKREPTISGIDAAIVSATKGRIGNRIAEVISSTTKREDRNEALDVLRD
                     EVVDSFLNEDEATDPKAVREVFADEIKKAVRHRILYDGIRPDGRDYVTIRPLSSEVGL
                     SPRAHGSGLFQRGQTQVLSIVALGTPREAQKLDGLSPEDTRRYMHHYNFPPFSTGETW
                     PMRGPRRREIGHGALAENALLPMIPSEEEFPYTIRVVSEVLSSNGSTSQASVCASSLA
                     LMDCGVPIKRPVAGVAMGLVTDGNKYAVLTDIQGLEDHLGDMDFKVAGTSDGITALQM
                     DIKISGLDMNIMTEALEQARVGRMEILDSMLATIAAPRKELSLYAPRMLTIKIDPEKI
                     GAVIGKGGATIRGLEEEFEVSVDIQEDGTVFVAGVDGLKAEAALERIHMLTHDPDLGE
                     IFTGKVVRITDFGAFVELAPGQDGMVHISQLASEHLQRVEDAVKMGDEVMVMVTDVSS
                     EGKIRLSRRAVLEGWTLDEARAQDAVRKGGGGPRGGNGDRGGDRRGGDRRGGDRGGDR
                     GGRPPRR"
     gene            complement(12088..12357)
                     /gene="rpsO"
                     /locus_tag="PLC06_03935"
     CDS             complement(12088..12357)
                     /gene="rpsO"
                     /locus_tag="PLC06_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007052997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRO89626.1"
                     /translation="MPLTVAEKAELIGNFATHDGDTGSPEVQIALMDKRIRQLQEHLN
                     THKHDESSRRGLLKLVGKRRRMLAYLRKEHPDRYRTIISRLGLRR"
     gene            complement(12521..13690)
                     /locus_tag="PLC06_03940"
     CDS             complement(12521..13690)
                     /locus_tag="PLC06_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89627.1"
                     /translation="MNHSKKLPVCPESDVLSLIEMKQVPVHCNVLWPTRGEAINAPRG
                     DIRLGYLPGCGHTCNLDFDPSLMEYTQEYENSLHFSPRFQEYATALAEGLIAKYDLHD
                     KDIVEIGAGKGDFLIMMCSKQGNRGWGYDPSYVPEEGYTAPNVTFVQDFYTEKYTHQR
                     ADLIVCRHVLEHIEDPDAFIAQVRRAVGDQKSVVFFEVPNSLWTLRQGGIWDVIYEHC
                     SYYSPLSLAYLFQRHGFKVLAVNEVFGGQFLTIEAVPDDGADLPQDASAELAQLTADA
                     RSFAAHYQAKRDEWQARLAALAGAGKRGVVWGAGSKGVTFLNAMATGEEIAAVVDINP
                     RKQGKYVPGTGQQIVPPLELVNIRPDFVIIMNANYRDEIGKMLADIGIGADILVA"
     gene            complement(13694..14839)
                     /locus_tag="PLC06_03945"
     CDS             complement(13694..14839)
                     /locus_tag="PLC06_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89628.1"
                     /translation="MSIFYTVKNVPVNSVLLVMSRDEALGFQTGDISLAACPRCGFIS
                     NIAFDEALTQYTERYEATQGYSPTFNKFHQALASDLIDQYDLRGKDIIEIGCDKGDFI
                     TMLCEMGENRGVGFDPAYVPGRHPSPAADRLTFIPDFYSEKYADYRADFICCKMTLEH
                     IPNVGEFVATVRRSIGDKHETIVFFQIPNARYVLCDVAFWDVYYEHCSYFTKGSLARL
                     FRANGFEVNKLWTVYDDQYLMIEARPTESASAAAPLADEESPADTLKMVEYFVEHYEA
                     KRADWQDKLREMKAAGKKAVLWGGGSKGVAFLTTLGQTLDDIAYAVDINPIKTGTFMA
                     GTGQEIVAPSFLQEYRPDVVIIMNPVYLDEITRDLHAMGLSPEIYTL"
     gene            complement(14982..>15512)
                     /locus_tag="PLC06_03950"
     CDS             complement(14982..>15512)
                     /locus_tag="PLC06_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HRO89629.1"
                     /translation="RKRRGEFIKQEHPAPGRVVLHYYLPLAEMIVDFYDKLKSSTRGY
                     ASMDYEFDSYRASNLVKLEVLVAKTPVDALAMIVHADDAYHRGQRLVSELKELIPRQM
                     YEVPIQAATGKRVISRANVKAMRKDVLAKCYGGDITRKKKLLEKQKKGKKRMKMIGNV
                     EVPQEAFMAVLKLGED"
BASE COUNT         3435 a         4499 c         4119 g         3259 t
ORIGIN      
        1 gccgaaaata acaatagaga ctatttcttc catcagtttt ctctcacgag ggcaacggct
       61 tgacgttgat agaagctatt gcccgaaata gcggggatat catccacgac ttaagccatg
      121 gtttatatga tagcaaaaag gcggtccggc cgatagccaa accgcctatt gatcaactat
      181 tcaacatcat tcagcgctaa atcttcgtga agctatcgag aacatccgcc acaagatcaa
      241 agcgaccaaa cgccggcata atgtagaagc cacggacata tggccgcagc gtttccgcaa
      301 tttcgcgagc aatctgaacc ccttcggtga ccggcttgtc ggccgcgcgc atccgttccc
      361 gcttttcttt gggaatatag atgcccggca cctcgttatg aagaaattca gcattgcggc
      421 tgttatagag aggcttaatg ccggcgatga tcggcggtgg ggcctcgccg taggtgcgtt
      481 cgtaggtctc caaaaaagag acggccgcca cgggatcgaa caccggctgc gtcagcgcaa
      541 agtccgcgcc atgttctacc ttcttgcgga gcaacttcag ttctgattcc acattcttgg
      601 gcgtcaagga cagggcacaa ccaaccacaa atgtagtggg ctggtcaatg gactggcccg
      661 ccttgtcgag gccatggttg aactgcttct tgaccaactc aatcaacccg gtaggcacga
      721 tgtcataggt atccatcgct tcgggaaagt caccgatgcg catgggatcg cccatcgtca
      781 ccagaaggtt ccgaatgccc atggcgtggg cagccagcag gtcgccctgg atacgcagaa
      841 tattccggcc acgtgtcggg aaatgcagca ccgtttccag gccgacatcc ttttggatga
      901 gataagcggc tgcccaggcg ctcattctca tgcgcgccag cgggctatcg gcaatattga
      961 gaaatgtcgc tccagcctcg cgcagcattc gcgctccggc caaaagccgc tccggcgcgg
     1021 cacccttggg cggatccatc tccacagtca ggatgagttg atcaccggca agatgctgcg
     1081 cgaggcgagt tggctcatca cctctaacga ccgtcgtttc cggccggctg gtgatgcgaa
     1141 tttcgggcag cgacatcgaa aactcgccag ggttatcaat cgcttcgcgc attgccttga
     1201 tatgctgcga agtcgtgccg caacaaccac caatcaggtt gacaccggcc ttcaggaagg
     1261 cgcgggcata gtcggcgaaa taatcaggtg tggccggata catgacccgc ccgccctgca
     1321 tttgctccgg ccaaccggca ttgggcgacg cggacacagg aatatcagga gccaacttgc
     1381 ggacgatacc ggccagacgc aagacctggg cggggccact gctgcagttg atcccgacag
     1441 catctacatc gagcgaggtc aggttcgtag cgatggcctc aggggggtaa cccaggagcg
     1501 tcttgtcatc acgtgtaaaa gtcatctgca cgatgatcgg gatatccttg ctgatcgatc
     1561 gcgcggcagc tacagccgct tcgatctcct tgatatccga catcgtctca ataatgatca
     1621 ggtccacacc ctgcggctcg gcattgatca gggcatcgat ttgttcggtg aaagcttctt
     1681 gtgcttcggc cgaagatacc ctgcccaggg gagccaggcg agcgcccaat ggcccaactg
     1741 aaccggccag cagcacttcc ttgaacgaac ccgcaatcac ccggcgggcc acggccacgg
     1801 ccgcatgatt gatctccacc accttgtcct gcttgccata ctcggaaagc ttgaaccgat
     1861 tcgccccaaa tgtgtttgtc tcaatgaggt cggagcctgc gtcgatatac gatcgatgga
     1921 tctcagccac gaccgccgga ttatcgaggt tgaccatatc aaacgactgg tcgatgggta
     1981 taccgcgccc gtggagcaca gttccaaaag ccccatccat caggatgggg ccgttgttca
     2041 cacgttcttt aaattcgagc cgattcatat tttcgtttca ccgctaatca aacttttctc
     2101 ctcttaacta tagctatatt tcttaagaga tcactgtcgt tatgcgcgac acaatatttg
     2161 agaaaagttc tttcacgagg agccactcga ttaattccca aacagctcct gaatcatcgg
     2221 catcgccacg tcactgttca gcaacaaaac cgaagctccg cccggcgtcc gaaagctggt
     2281 gacgtaatcc tgatccagca cgcgattttg cgtattgtcc gacgggatat cactaactgt
     2341 cttggccagg cggacgagct gctccagatt caggtcagtg cgtattcctc cctcgacctc
     2401 acgatacaaa gacggcgctc gcaagatcat ctggccgata cccaggctga gcgctttgct
     2461 gcgcgcggca aaaagcacct gctgttggcg ataggcgcgg ttgaaatcgc tgtcggcgtg
     2521 gcgcgtgcgc gcatatttga gagccatttg accatcaaaa tgctgcatgc cggccggaat
     2581 gaacaatgga tcgtaaccat aatccatgtc gggatagagc gagtcgttga tatcataagg
     2641 cacctggacg tcgatgcctt ccaacagatc gactatccgg acaaacgcgc cgaagtccac
     2701 catgacgaaa tgattaatcg gaatgcccag gttagaggaa accgtctgca tggccaatgc
     2761 ggcgccatcc agataatcac cttctcgcgc tccatagacc agggccgtgt tgattcgatc
     2821 ctgaccatag ccggggatct ggacataaag atcccgcgga atcgaaagaa tcgacatcga
     2881 atctgcctca ggatccaccg aaatcaccat catcgagtca gtccgggaaa caaacgcctc
     2941 gcccgggcga cggtcgatac ccaccagcaa gatattcacc cgatcgttgc cttcccaagc
     3001 cccaatcgta ggttggacag caggctcagc ggtcgcacct tcaataatgg tcacaggggc
     3061 taatccgcca tcaacagacg ttaagcctcc gtcaattcct tcggccgcgg tttcatcgct
     3121 atccggtgca gtcatcaaat gttgcactgt gagataggcg aagatcaata cggcaatagc
     3181 agacaaaaca aacgcgaccg ataacagcgc gctcaaccaa ccgggtaata atggctgtcg
     3241 attcaacttt acactcaaac ctgctcgtaa ttgggcgtcc tcaatgagag ggcactttta
     3301 acattataca ttcataacga ccggggagcc ttgctggttc ccgtcgggtt gcctctgatt
     3361 cttctacttc tcataaccca tttattatga tattattgat aataatgctt ggccgagatt
     3421 gacgataacg tcagcactcg gtacagtttg tctggtgcgg ggccgcccac cggcttcgcc
     3481 taatcagcat cggattattg ataattacca tggccctgga tcgctacgga cgaaatatta
     3541 attacctcag aatctcgttg accgaccggt gcaatttgcg ctgtgtctat tgcatgccgg
     3601 aggacatgac ctttcggccc cgaagcgagt tgatgcagga tgatgaagtg attcgactgg
     3661 tacggctctt cgccgacctc ggctttcata aattccggct caccgggggt gagccgaccg
     3721 ttcgtgccaa tatcgtggag atcgtccgtc agatcgcggc gacgcccggc gtcgaaaagg
     3781 tcgccatgac aaccaacggg ctgctgctcg atcgcctggc ccaaccgctg gtcgatgccg
     3841 gtcttaatcg ggtgaatatc agcatcgaca ccctcgatcc ggccaaattc aagaagctca
     3901 ctcgctgggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atatcagcat
     4021 cgacaccctc gatccggcca aattcaagaa gctcactcgc tggggcaatg tcgaggatgt
     4081 atggcgggga atacgcgcgg ccgaagccac ggacctggaa atcaagctta atgccgtggt
     4141 ggtgcgaaat tataacgatc aggaagacgt agttgatctg gcccgtttga ccctctaccg
     4201 gccatggcag gtccgattca tcgagatgat gcctttcgga gagatcagcg attttcaatt
     4261 agccggcgtt gtgacacaag aagagatcat caaaacgatt gagtccggtc tgggagcgct
     4321 aacaccggtc aacccgggtg agctggacgg tgaagccagc ctgtactatc ttgagaatgc
     4381 caaagggcgc gtcggtttca tcagctcggt gagccatccg ttttgcgcca gctgcaaccg
     4441 cacgcgattg acggccgacg gccgcctgcg actctgcttg ttgcgggaga aggaagtcga
     4501 tctgctgact cccatgcgcg atggcgctac agacacagaa atgctggcca tcatcaagga
     4561 aggtatctgg tggaaaccct gggggcatca actcgccgaa gatatcgtcc cgctcaaccg
     4621 ggtgatgtcg gagattggcg gatagtgctt gccctcatcg gccgttcgtg acgctgtttg
     4681 gccccatcgg cggggcgaaa cgtcctaagg gcgcttgcgt caacccccaa ctctcctgaa
     4741 tcaggtagag aggccggcct ttcacttcgt catagattcg gccgagatat tcgccaataa
     4801 tccccagact gatgagctgg acactgccaa gaaacagaac aaccaccaga gtagtggcct
     4861 gccccaacag tggctcatgg ggccccagca agcggacggt gaccacgcca agtatcccca
     4921 gcacactaat cccggcaatg ataaacccca atatggtcgc cagctgcagt ggaaaatagg
     4981 agaaactggt gatcgcatcg agagcgaacg gcagcatttt cttcacgctg gtgaacttcg
     5041 cctcaccggc atagcgcgcc tcgcgattgt attctacgcc gatctgccga aatcctaccc
     5101 agggcaccat gcctcgcaga aaccggttgc gctcgttcat gccgcggatg gaattgatca
     5161 cgcgtcgatc catcagacgg aaatcgccag tgtccagcgg tatattgata ctggtgatgc
     5221 gatggatcag acggtagaag agcttggcgg ttgtcagctt aaaccacgtc tcgccctgac
     5281 ggctggcccg aacgccgtag accacatcat aaccggcgcg ccattgcgcc agcatggccg
     5341 gatagacctc ggggggatcc tgcaggtcgg cgtccgtgag gatgacggcg tcgccctgag
     5401 aaaagtccag accggccgtc gccgctatct ggaagccgaa attgcgcgaa aagctcagtc
     5461 ccttcactcg cgggtcacgc tcgtgtaacc gggcgatcac ccccgccgaa tcatcgcggc
     5521 tgccatcatt gacgaggatg agttcccaac tctctcccgc accatcaagc acatcccgaa
     5581 cgcgctcata taacgcatcg agaacgcttt cttcgttata gacaggagca acgaccgaga
     5641 ttaagtcctt acgattttcc acgggtgtgc tatacctcta atccgatcaa taatcccaca
     5701 tgctcggacg gatataaagt atcatcatcc ggcgctgatt tgtcgccaaa tatggcggct
     5761 cccgtcacct tatacacggc cggagacagg aaaacgtaat caaggcattc agcggcaacc
     5821 ggctgcggct gaaccagatt cgacggataa gtcgccagcg gatcgcgacc gtgcacctct
     5881 gcaaaggccg aacgatagga ctggtacatg aaagtgacgg caatcccttg cgaggattca
     5941 ttaaaatcac cacaaatcac ctgcaacggc acacgtttct tttcgttgat ccagcccacc
     6001 aggttcattg cttgctcggc acgtgcttca aatgcacctg ccccatgatg cagttgaacg
     6061 ttgacaaagt caagagactg ccgccgcgag ccggccgccc ccgcgggcag ctcaagattc
     6121 gctcgaagcg ctattcgtcc gtcaacccct aactccttgg tatcatgata gatgatgggc
     6181 aggcgggtca taatccccat ccccctggac caatttcgca accccgaacg gcggccctgt
     6241 acaattcggt aaggtcggcg gccgtcgccc gatgtcctga tgttgacctg ccgcgccagc
     6301 cagctcccct ggccgataga tagatcaatc tcttgcagag cgatgatatc cggcagttga
     6361 tccagaagct ggccaaccag caaatgacgg cgagccaacc accggtttgt ccggccacgg
     6421 agattgagag tcgccaccgt aaagccgtac ataggcacag ggattcgagc tacttgattg
     6481 agagatcaaa ttcaccggcg gggacagcgg cctggacaat ctctttatca atatggacgg
     6541 taagggtgaa cggcacatca gctcgcacac gaatcgaatc attgctgcca atgaggtcga
     6601 gcgaaccatc attaccgaga gacaggttgc gaaccccata cgcttcatca gtggtcagta
     6661 ttctacgcca ggtcacctga cgcaatggcc agtcaatggc caaacccagc acctgttcca
     6721 ggatcatcgg aataatggac gccggcgtga tgccggaaat atcctcgact tgcggctccg
     6781 cggggccaag ctcttcaggc agataacttt cccagaattt tcccgtcatt tcaaacactt
     6841 tgtcaaccat atcgagatgg accagggcca acttgtgccc caatctgtgc tgatcggcaa
     6901 cacccaggcc gcgaagcacc atataagtaa gtgccggtcg caccgctcca cgccatccgt
     6961 taccggttcg cgcattgtag gcctcgctgt cggccgagag gctggggatc acattttcgg
     7021 tgcggaacga ccacgtgtcg cgcaagtgtt gaataaacaa ggggagcctg tccttgggga
     7081 caagatgtgg atcaagcaaa gcccaatagg atgcgatact ctttactgga ctaaaagaac
     7141 gatccggccc gatgtcctgg taaaaattaa gcgctccgtt ccacatgttc tcattgattg
     7201 tcttcacgag ccgctctcgc tcggatgaga cttcctcagc caattcttct tcgccaagca
     7261 aaacggccat ccgctccagc atggtgcaat cgaggacggc ctgggcgttg atgtctatcc
     7321 aggcccagtg ttggtgatga tattggccat cggggacgcg aggctgattc accaggctgc
     7381 tggcataggc cgtcgtccaa tagaggccgt tcggccaggt acgattagcg cgacaccaac
     7441 gatgataggc catcaacgga tagaagacgg ccgcgatgcg ttccttatcc ccggtcaatc
     7501 gaaaatgacg ccattcagtc caccccatcg tgttagggcc ggtgctgttg ggatcatagg
     7561 attcgtggaa atcttcgccg gtttcggggt ccaattcgcg acagataaac ccgttgtcat
     7621 gttgaccggc atagaagttg tcgaggaatt cgatcaggtt gaacgagccg gggatatagc
     7681 ccgccgcatt ggcgatcagc gcgctccggc ccatatggat cttccggcca gggttgggct
     7741 gattataacc cgcgaccaga ccggattcga tcgaaggctg atgcaaggct gcccaaaatg
     7801 aacgccaggc ttcccaatac aagccaggca agtttgaatc ttcgggcaac accgggattg
     7861 gcaggcgagg gcgaacttct tcgaaatcag gtattcgctg cggggtgcgt ccacgctgcc
     7921 tgaaactgtt tttctccacc aggggatcgg caggtaaaga aaaacgtgcg tccacaatag
     7981 gctcctgtca aatcaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     8101 atcggcaggt aaagaaaaac gtgcgtccac aataggctcc tgtcaaatca ggccggtcga
     8161 gtatctgcgt actcgaccgg ccatatcaat ccgttagggc agaatctctt cgatggtaat
     8221 ccgcgcgatc gtgtcgcccg caggcgcata cgggttacgc gagggatctc gtggcgtgat
     8281 tcgctccacg acgtccatcc cttcgatcac cttgccaaaa atcgtgtaac tgccatcgag
     8341 ctggctcacg tcgccatagg tgatgtacca ctgactgccg ttcgtgttag gtccggcgtt
     8401 cgccatggcg acaactccct tctcgtcatg cgacaatgag gagtcgatct cgttcgggat
     8461 gtaatatccc ggcccgccgc gccccgtgcc gctgggatcg cccgtctggg ccacaaagcc
     8521 ggggataacg cggtgaaaag tgatgttgtc gaaccagcca ttcctggcaa ggaacacaaa
     8581 actgttcact gtttgcgggg ccgacttggg cagcagttcg atcttgaacg tgtcccctga
     8641 tcccatctca accgtcgcca cataacgggc gtcgggatta attgtcattt ccggcgccga
     8701 cttgaattgt cgagctgcca gttccttcaa gctgatttcg tctttcacaa aagcctccca
     8761 ggcttcgaat tcgcggggaa gtgattcacc cgcgacgatc tgtccattca caattgctga
     8821 cggggtgccg ggcaaaccca gacccatggc ctcctgctcg gaagccgaga caaggtcagt
     8881 gtaagtaccg tcgtccagtt ccgtggtgaa acgtgaaaca tcaaggccca gttcttcagc
     8941 caacgctacg aagaaatccc gcgcggcggc gctatccagt cctgcccatt gatcctgtcg
     9001 cgagaacaac aaatcatggt attcccagaa cttgccttga gcgccggcag cctcggcagc
     9061 ctcggccgat ttttgcgcgt taggatgaat actagtcagc ggaaagtggc gatatatgat
     9121 ctgaatctgt tcaggatagg cgtctaccag acgccccagc aggggagcaa accctgcgca
     9181 gcccggtcac tgaaaatcgc catattcgat aatcgtcacc gcggggttcg atgtgttgcc
     9241 ctgcttccaa tcccgttcac gagcttgcag aggatcgtct atgctggaga tagccgtgtc
     9301 actagcctcc actcctgatc cttcagccgg ctctccggtt ggagaggatt ccgtcaccgc
     9361 tgcctgctct tcacttcctt cctgttcggc cacttcggct cctccggagc cacaagcggc
     9421 gataaacaag gacagaagaa tgagcagcaa tccgatcttc ttcatatcag attactccta
     9481 tatccgtgtt ccaaaagaaa aacaggcagc cgaggcatgt ttcacttcgg ctgcctgagc
     9541 acgttaaatg gattaacgac gcggcgggcg gccgccgcga tcgccaccac ggtcgccacc
     9601 tcgacggtcg ccacctcggc ggtcgccgcc tcggtcgcca ttgccgccgc gcggcccgcc
     9661 gccacccttg cgaacggcat cctgggcgcg agcctcgtcg agggtccagc cctcaagcac
     9721 ggcgcgacgc gagaggcgga tcttgccttc gctgctgacg tcggtgacca taaccatgac
     9781 ctcgtcaccc atcttgacgg catcctcgac gcgctgcaga tgctctgagg ccaattgcga
     9841 gatgtggacc atgccgtcct ggccgggagc cagctcgaca aacgcgccaa agtcggtgat
     9901 gcgcacgacc ttgcccgtga agatttcgcc cagatcggga tcatgagtca gcatgtggat
     9961 gcgctccagc gcggcttcgg ccttcaggcc atccacaccg gcgacaaaca ccgtgccgtc
    10021 ttcctgaata tcgaccgaga cttcgaactc ttcctcgagg ccgcggatcg tcgctccacc
    10081 cttgccgatg accgcgccga tcttctcagg atcgatcttg atcgtcagca tgcgcggcgc
    10141 gtagaggctc aattccttgc gcggcgcagc gatggtagcc agcattgagt cgaggatctc
    10201 catccggccc acccgcgcct gttccagcgc ttcagtcatg atattcatgt ccagaccgct
    10261 gatcttgatg tccatttgca gagccgtgat gccgtcgctc gtgcccgcaa ccttgaagtc
    10321 catatcaccg agatggtcct caaggccctg gatgtcggtg agaacggcat atttgttgcc
    10381 gtccgtcacc aggcccatcg ccacgccggc taccggtcgc ttgatgggca caccgcaatc
    10441 catgagcgcc aggcttgagg cacagacgct ggcctggctt gtggacccgt tcgatgagag
    10501 aacctccgac acgacgcgaa tcgtataggg aaactcctcc tcagacggaa tcatcggcaa
    10561 cagggcgttc tccgccagcg cgccgtggcc aatctcgcga cggcgtgggc cgcgcatggg
    10621 ccaggtctcg ccggtggaga aaggcgggaa attgtaatga tgcatataac ggcgggtgtc
    10681 ttcaggcgac agaccatcga gcttctgggc ctcgcgaggt gtgcccagag caacgatcga
    10741 caacacctgg gtctgaccgc gctggaacaa gccggagcca tgagcgcgag ggctaaggcc
    10801 cacttcggac gagagcggcc gaatcgtcac gtaatcgcgg ccgtcggggc gaatgccatc
    10861 gtacaggatg cgatgacgca cggccttctt gatctcgtcg gcgaacactt cacgcactgc
    10921 cttcgggtca gtcgcctcat cttcattcag gaagctgtcg acaacctcgt cgcgcaagac
    10981 atcgagcgcc tcattgcgat cttcgcgctt ggtcgtggag gagatgacct cggcgatacg
    11041 attgccgatg cgccccttgg tcgcgctaac gatggcggcg tcgatcccgg agatggtcgg
    11101 ctcgcgcttt tccttgccga cggccgcctg catctcttcg agcagagtga tgatgggctg
    11161 tagcgcctga tgaccgaaag ccaaggcttc aaccatcacc gcttcgggaa cttcattagc
    11221 ggccgcttcg accatgataa tggcgtcgcg tgaaccggca atgcgcaagt cgaggttgct
    11281 ctcagccatc tcctggatgg tcgggtcggc cacgaattgc ccgtcgatat aaccgacgcg
    11341 aaccgcgccg accggcccgt tccagggcac atccgagatt gtcagcgccg cgctggcggc
    11401 attgaccgac atgatatcca gatgatgcac gctgtcggat gacagggttg tgacgatcac
    11461 ctgcacttcg ttgcgcatcc catccgggaa caggggacgc agcggccggt ccgtcaggcg
    11521 agatgtcagg accgcctcag tggtcggctt gccctcgcga cggaaaaagc caccggggat
    11581 gcgcccggca gcatacatct tctcttcgaa atcaacgctg agggggaaga aatccagtcc
    11641 ctcgcgcacg tttttcgaca tcgtagccgt ggccagcaac atcgactcac cgacctgcag
    11701 cgtaatcgcg ccaccggctt gctcagccag cttgccggtc ttgatggtca tattgtgcct
    11761 gcccaccatg gctgaaaata cagattcaga tcccattcta tttttcctta ttcagtattc
    11821 aatcgcgaga ccaactatcc aaaaggcgaa acaacgaaca agcagccatt tcggcgctgc
    11881 catgctcgtg cttcttcatc tttgggacaa gcgatcccgc ataactcgat tgggggagca
    11941 gatatgggat tagggactgg ggattggctc ttacaccccc atcggcgtaa gtgacaatac
    12001 ccaattccta aacccgtccg tttatttttc tgctcctgga aaaagggcga agtcgacttc
    12061 gccctttttt cgttattcca ttatcaatta acggcgcaga cccaggcgac taataatagt
    12121 ccgataacga tcggggtgtt ccttgcgcaa gtatgccaac atcctgcgcc gcttgcctac
    12181 cagtttaagt aacccgcggc gggaactctc gtcatgcttg tgggtgttca agtgctcctg
    12241 gagctggcga atccgcttgt ccatgagcgc tatctgtact tcgggcgaac cggtgtcgcc
    12301 gtcgtgggtc gcaaaattgc caattaattc ggctttctct gccacagtca agggcatgtc
    12361 aatcctccgt agaaatagat attttcaaga cgaatgaggt aagtcacctc agccgagagg
    12421 ccgtcgtgcc gataacgtcg tacctttccg ccagaggggt attataacca aggaatgccg
    12481 ggtgacaagc acaccgacga ttctctcagg tggcgtggca ttacgcaacg agaatatccg
    12541 cgccgattcc gatgtctgcc agcattttgc caatctcatc acgataattg gcgttcatga
    12601 tgatcacgaa atcgggccgg atattcacca gctccaatgg cggcacgatt tgttggccgg
    12661 tgccggggac atacttgcct tgcttgcgtg gattaatatc gaccacggcc gcgatctctt
    12721 cgccggtcgc catcgcgttc aggaaagtca cgcccttcga acccgcgccc cagacaacgc
    12781 cacgcttccc ggccccggcc aacgccgcta accttgcctg ccattcatcc cgcttggcct
    12841 gataatgggc ggcaaaggaa cgggcgtcgg ccgtcagttg cgccaattcg gccgaggcat
    12901 cctgaggtaa atctgcccca tcatccggca cggcttcgat ggtcaggaac tgtccaccaa
    12961 acacctcgtt gaccgccagc actttgaagc catggcgctg gaagaggtaa gccagcgaga
    13021 gcggactgta ataggagcaa tgctcataga tcacgtccca gataccgcct tggcgcagtg
    13081 tccacagcga gtttggcacc tcaaagaaca ccaccgactt ctgatcgcct acggcgcgtc
    13141 gaacctgggc gatgaacgcg tcggggtctt cgatatgctc gagcacgtgg cggcagacga
    13201 tgagatccgc gcgctggtgc gtgtatttct cggtgtagaa gtcctggaca aaggtaacat
    13261 tcggcgcggt gtagccttct tcaggcacat agctgggatc gtacccccaa ccgcgattcc
    13321 cctgcttcga gcacatcata atcaggaagt cgcccttgcc ggcgccgatt tcgacgatgt
    13381 ccttgtcatg cagatcgtat ttggcgatca ggccttcagc cagggcggtc gcgtattcct
    13441 ggaatcgggg tgagaaatgg agcgagttct cgtactcctg cgtgtattcc atcagcgaag
    13501 gatcgaaatc caggttacag gtatggccgc agccgggcag atagcccaag cgaatatctc
    13561 cgcgtggcgc attgatcgct tcccctcgcg tcggccacag cacgttacag tgaaccggta
    13621 cctgcttcat ttcgataaga gagagcacgt ccgattcggg acaaaccggc agtttttttg
    13681 agtgattcat cgtttatagg gtatagattt caggtgacag gcccatggcg tgaagatcgc
    13741 gggtgatctc atccagatag acggggttca tgatgataac aacatcgggc cgatattctt
    13801 gcaggaaaga cggcgcaacg atctcctgcc ccgttccggc catgaacgtg ccggttttga
    13861 tcgggttgat atcgactgca taagcaatgt cgtccagtgt ttggcccagc gttgtcagaa
    13921 acgccacgcc cttggagccc ccaccccaca acaccgcttt cttgcccgca gctttcatct
    13981 cccgcagctt gtcctgccaa tctgcccgct tcgcctcgta atgttcgaca aaatattcaa
    14041 ccatcttcag ggtatcggcg gggctttctt cgtctgccag aggagcagcg gccgaggcgc
    14101 tctctgtcgg gcgggcctcg atcatcaaat actgatcatc atagaccgtc cataacttgt
    14161 tcacttcaaa gccgttcgcg cggaacagtc tggccagcga gcctttcgtg aagtaggagc
    14221 aatgttcgta gtagacatcc cagaaagcca catcgcacaa cacatagcgc gcgttgggta
    14281 tctggaaaaa gacgatggtc tcatgtttat cgccgatcga gcgacggacg gtcgccacga
    14341 attcgccaac attgggaata tgctccaacg tcattttgca gcagatgaaa tcagcccgat
    14401 agtcggcgta cttctccgaa taaaagtcgg ggatgaacgt caggcgatcg gccgctgggc
    14461 tgggatggcg gccggggacg taggccggat caaacccaac cccccgattc tcgcccatct
    14521 cgcacaacat cgtgatgaag tctcccttgt cgcagccgat ctcgatgatg tccttgccgc
    14581 gcagatcgta ttggtcgatg agatcactcg ccaacgcctg atggaacttg ttgaaggtcg
    14641 gcgaatagcc ttgcgtcgct tcgtaacgct ccgtgtattg cgtcagcgcc tcatcaaagg
    14701 cgatattcga aataaagcca catcgtggac acgcggcgag agagatgtcc ccggtctgaa
    14761 accctaatgc ctcatctcgg ctcatcacga gaagaacgct attcaccggg acattcttca
    14821 cagtataaaa tatggacatg ccgggcgaac cgcaggccgg acatgcatgg tcagcgcttt
    14881 tcatgagcgt acctcgattg ataaattagt ttgcgaaagt ggggcgaaaa ccttgaccgg
    14941 ccacaggggt ggattgtggc cggcgtcgtt cgttaaggag tttaatcctc gccaagcttc
    15001 aacacagcca tgaacgcttc ctgcggcact tccacattgc cgatcatctt catgcgtttc
    15061 ttacccttct tctgcttctc gagcagcttt ttcttccggg taatgtcgcc cccatagcat
    15121 tttgccagca catccttgcg cattgcctta acattggcgc gactgatcac gcgcttgccg
    15181 gtggccgcct ggatcggcac ctcatacatc tggcgcggga tcagctcctt caactccgac
    15241 accagccgct gaccgcgatg ataggcatcg tcagcatgga cgatcatcgc cagcgcgtcg
    15301 accggcgtct tcgccacaag gacttccagc ttgacgaggt tggaggcgcg gtaactgtcg
    15361 aactcgtaat ccatagaggc ataaccgcgc gtgctgctct tcagcttgtc gtagaagtcg
    15421 acgatcattt ccgccagagg caggtagtag tggagcacaa cgcgacccgg cgcaggatgc
    15481 tcctgcttga taaattcacc ccgtcgcttg cg
//
LOCUS       DAOMQG010000039        15387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1674_length_15387_cov_9.088703, whole genome shotgun sequence.
ACCESSION   DAOMQG010000039 DAOMQG010000000
VERSION     DAOMQG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_15387_cov_9.088703"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="tal"
                     /locus_tag="PLC06_03955"
     CDS             <1..933
                     /gene="tal"
                     /locus_tag="PLC06_03955"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRO89630.1"
                     /translation="PLAATGRSPEEIFWELAIEDIRNAADLFRPLFDATHGGDGFVSL
                     EVGPGQANDTEATIAETQRLWSLTDRPNLMIKIPATLAGIPAITTATAHGLNINITLI
                     FARERYAAVMDAFLSGLEQRVAAGLPIDHIASVASFFVSRVDTNVDKRLEAIIGAGGP
                     QAAEARSLLGKAAIANAKLAYADFQRVFSSDRFAALKAKGAQVQRPLWASTSTKNPAY
                     RDVIYVEDLIGPDTVNTVPQKTLTALLDHAVLAQTLTTGVDEAQTVIDRLAALGIDMA
                     DVTHELEIEGVQAFTDAFHTLLASIEKKLAVVAA"
     assembly_gap    968..977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1014..1697)
                     /locus_tag="PLC06_03960"
     CDS             complement(1014..1697)
                     /locus_tag="PLC06_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRO89631.1"
                     /translation="MSIISNWLARSPVFGVLDKSARAELARQAIHKRFEKGEWIAPYG
                     ETWDYLFLVGSGAIHALKKSPEGRSLIVLQMKPGDLYWGAAFFVEFAPMPMLLEAQRP
                     TDVYLWPRDYLQPLLQEDGRAMWELSTMMARRILQAGDFLEELAFQPVKRRLARMLLD
                     LYGDTAGEYVSRGRTLDEMAALIGTKREIVCRLLYELADEGLIDITRTKLRIADRERL
                     MEAARQAGG"
     gene            1847..3406
                     /locus_tag="PLC06_03965"
     CDS             1847..3406
                     /locus_tag="PLC06_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89632.1"
                     /translation="MNALTKRIDYLFRSTAGLILVAIAVVSLITAIWGTLSGPMVEWG
                     IRDVTVRLLGMQLVQADREARIIMLYHTIAMTIVAIEVYIITDILPMKRHEQVVINAT
                     ITIGYLTSVIFGMLFAYWGQNFLFHGLFLLGQSLVFFAGILLAAALWPWKKAYRLAAD
                     SPYAHTRGGVDLERVAFFAMAIATLLSAAFGAVTGSYWGNGHETFLAEDLIRAPLKTG
                     LQRAIIGHLHIMLTLIAIAITLIVGRWMDFKGRLHKVAMILMIIGTIIITFGALSVVW
                     VEWAHVTIYVGSVFVMLAALLFVIYSWRKLIHDRTAELGLVKPTLGQKVKALLHDPLK
                     FGPGWQMVFMNFTVSGIGIFMAVKLDEIFRVWPHREERITLTGHWHILASLIATIILM
                     YFADRAGLKGRARKWFGWTLIIMSNLAFAAVTVFSMKRLFVSEGAQQPIVNWTMLLAD
                     IGLATVLIALGIFMAWRLIDLFRARGRWTKELREERERVTRAEIAEKQRRLEELNQTL
                     EEVSTPRGGAA"
     gene            3403..4362
                     /locus_tag="PLC06_03970"
     CDS             3403..4362
                     /locus_tag="PLC06_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRO89633.1"
                     /translation="MRTIIFLLILLLSVTACGPAPNIAAITPPYIETGIDADAWATVP
                     AGDFPYGQHDHMTPVDYDYEIMVTLVTNEQFAAFLNEALAADVASLGTVEVEAGESVW
                     TEEGVFGPYPGDPFDAYKHEEEIRAGDKLWMSTTAEGARITFDGQAFVAQDAYRNHPA
                     TMVTWYGAKAYCEFYGGRLPTEIEWEKSARGTETIDGRGRPFPWGETIERNNANFYSS
                     FDLFVKMFGKLGNTTPVGFYNGRTYDGYQTLDSASPYGLYDMAGNVAQWTGDDYPYQH
                     YRYLRGGSFYSYEVDLRTWKSNSAGPQYFGPAVGFRCARDSNP"
     gene            4379..5242
                     /gene="cyoE"
                     /locus_tag="PLC06_03975"
     CDS             4379..5242
                     /gene="cyoE"
                     /locus_tag="PLC06_03975"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRO89634.1"
                     /translation="MATNLRATISRYTPLFKSLQTGLLLLTGLAGYMSARCPIFNVFT
                     VLAVAGSLLLAISGSTILNMWYDRDIDARMERTAGRPIPSGQVSPVEALRLGLLLSLA
                     GVVWAVTLDPLYGVIVFAGLFFDVVVYTIWLKRRTPWSIVWGGLAGGMPILAGRALGM
                     GQVEWVGVALALAVLFWIPTHILTFAARHHEDYRRAGVPTVPAVYGFAINRAIIAVSS
                     ILAAVMMGIAAYGIGLEVGYLRVMVVLSIGLLTLAIRSLMRPSERINFGLFKYASLYM
                     LGTMLLMMFVQ"
     gene            5272..5868
                     /locus_tag="PLC06_03980"
     CDS             5272..5868
                     /locus_tag="PLC06_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89635.1"
                     /translation="MSAIDRVFLLLTGLLAAYQIAIGVEGVPAVSLWAYTVGFGILLL
                     AGIMLIILGFEGLDSPFVAIVSTAAPLALSLGLVWDYLPAWRVAYLVFVIVGFIAVTI
                     TRVARMGRVATITLAVVHGVAGLIIFGLPIYLSLTGTTPGGFILVGIGGALIGVGGLM
                     LSFLKMGKPVLSQSAILTILPGLLFLMTAAFVAGFTFS"
     gene            5884..6567
                     /locus_tag="PLC06_03985"
     CDS             5884..6567
                     /locus_tag="PLC06_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89636.1"
                     /translation="MFGKILRVVGIILMALTAVMTLLGGIGTTCVALDAAKYGDSMAA
                     LADYQWLYMLYVLAGIVLAIAGLHATRLLIKSRANAYRMAMIVLVSGMVVGIIHMLTS
                     RALRGSSMPVDFIVYITAFTLVVFLLFRIPGIWQKVNLTGKDDHTTGMGAGVAMIVAG
                     FIILTVQVWGGAMHTINGVNYADVWHTQLALAGSAVLLAGSIVLLRKVLGVSLPRPSQ
                     VTVAKEVIR"
     gene            complement(6713..8545)
                     /locus_tag="PLC06_03990"
     CDS             complement(6713..8545)
                     /locus_tag="PLC06_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HRO89637.1"
                     /translation="MIRKVHIALVLLCVAGLTALGGRVALGARDGASRILLPVVLSAV
                     GYDIAIEADGFRPHVLAVAGGEIVEWANLSGSAQTLTAANDAFVSPAIAPGGRYQWAP
                     TGSGVYPYTAGGAAGLEGAIVVSPNGAADFFDGRTVGEAYRDSCGACHGADRQGAIGP
                     ALVPESLTASDSFYIDTIRNGRPGTPMPNWKYTFTDAEIWLMLGFLRSPVDESEATWE
                     MDDIIASREQLVEESTLPPVPTHTGNMDNLMLVTEREAQRIAVFDGDTHTLLGRVDSG
                     YRAHGYAFDPANERWAFNIGRDGWLHKIDLYSLQPVSRIRVGLDSRGLAISDDGKYVI
                     AGNFAPATAVILDAATLQPLKLLHTEGYGLDGEWVKSRVAITSDVAPELVGPYFIIAL
                     KEAGQMWRIDYSQPDFPVTKLADVGRILHDGFLSPDNKRFYIASQGDNQMVVIDVENW
                     KVVARINTGDTPHPGSGATWEANGAEYGATTHAGEGLVTVWDLADNEIVAKIPTSGPG
                     LFIRTHDQTPYVWADTMFAAVPNRIYVIDKASHAVVHVIEEGTQPLHPEMTNDGRYVY
                     IADWQDEVIRVYDAETFARVAVLDGLDTPTGIFNTSRRDERLGH"
     gene            complement(8680..9213)
                     /locus_tag="PLC06_03995"
     CDS             complement(8680..9213)
                     /locus_tag="PLC06_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRO89638.1"
                     /translation="MDNQLKALREQIDDFMQFHPQSPLADDQRDGFKGLDYYGENPAL
                     VIVATVEHLPDDEPIIEMETSTGDARPFRRWGTFAFEVDAQPARLTLYSDPTGEELFL
                     PFRDATSGNETYGAGRYLDNHRPGLRRLGEDEIEVDFNYAYNPYCAYSSYFSCPLPPR
                     ENWLAVPIRAGEKDFRS"
     gene            complement(9257..10060)
                     /locus_tag="PLC06_04000"
     CDS             complement(9257..10060)
                     /locus_tag="PLC06_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRO89639.1"
                     /translation="MPYAQTNNINLYYELHGRGEPLVLIPGLGYDGWMWHRMIPGLAE
                     RYQVIRIDNRGVGQSDKPPGPYTAAMLAADVAGLLDALDLPKAHIMGHSMGGFIAQAF
                     ALDYPDRVDKLILSATNFGGPRHVPISAEAMAVLMNMSGDPIERLRRGIAVSCAPGFV
                     EANPEFVEQWVNYRVAHPIDPAGYQAQLAIGLGLIAEAAAFEHRLTAVTAPTLILFGA
                     EDKVVPPANAPLLAGRIPGAQVEILPHAGHFFPFETPAAANAAVISFLG"
     gene            complement(10379..11935)
                     /locus_tag="PLC06_04005"
     CDS             complement(10379..11935)
                     /locus_tag="PLC06_04005"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRO89640.1"
                     /translation="MTSENHYPQGAADLLRARAELTPDRVALLDVHTGVEYTYRQLND
                     RANRLANWLRGLGVGPGDRVSILAQNSVHTVDLLYGLGKIGAILAPLNWRLTARELAW
                     IAADCAPKVLICGPEHTSLLAELRREVAFPVVVGIEGAAIEGALAYEACVAAAPVAEP
                     PPPPPAGEDICCILYTSGTTGRPKGAMISHRQVLWNCINTVISWELTADDISPVMTPM
                     FHAGGLFIFLTPLFYVGGKILLARSFDSEASLALIQRERATVVLGVPTLFQMWLGSPS
                     FTAADFSAVRYFVSGGAPCPMSLIAAWRAAKGGYLRQGYGLTEVGVNCFTMTNEESVA
                     KVGSVGKPVFHSRMRIVDEDGRDVARGETGELIIYGPHVCSGYWRNPEATARALRDGW
                     FHTGDMARQDADGFYYMAGRYKDMIISGGENVYAAEVETVFLEHPAVREAALIGHPDE
                     KWGEVGVMIVVARPETATTAGELLAFCRDRLARYKVPKQVIFAESLPYSPYGKVIKAE
                     LREKYARSDE"
     gene            complement(11954..12718)
                     /locus_tag="PLC06_04010"
     CDS             complement(11954..12718)
                     /locus_tag="PLC06_04010"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRO89641.1"
                     /translation="MRLKFKICIITGGAAGIGRATAQRFIEEGASVIICDVNEEQGHK
                     TAEELGPAARFVKVNVTDRAAVGAFVDEVVEQYGRVDVLVNNAGVLRDGTLVKMKDGV
                     LVKQMSEADFDLVISVNLKGVFNCTQAVAPHMIRQMSGVILNASSIVGLDGNFGQTNY
                     VATKSAVIGMTKVWSRELGRYGIRVNAVAPGFTSTEMVAAMPENILAGMKAHVPLGRL
                     GEPRDIANAYLFLASDEASYISGDVLRVDGGIVVGT"
     gene            complement(12751..13815)
                     /locus_tag="PLC06_04015"
     CDS             complement(12751..13815)
                     /locus_tag="PLC06_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO89642.1"
                     /translation="MSPIPILPGITSRMVATARINTHVLFSGPDDGQPVVFVHGNASS
                     ATFWEESMLALPAQYRGIAPDLRGYGDTEDLLVDATRGAKDWSDDLRALSDALGERPA
                     HLVGWSLGAAPILQFAIDNPALVKSLTLVNPISPYGFGGTKGEDGVPCYDDCAGSGGG
                     SVNPEFVRRMQIHDRSGDDPNSPRNVINAFYYKPPFRAAREEEFLSSLLSEKTGNDRY
                     PGDLTPSVNWPNVAPGVYGPINATSPRHLNLSGIVDMPHKPPILWIRGDSDQIVGDMS
                     LFDFGTLGKLGAVPGWPGDDVFPPQPMLLQTRTVLRRYVAQGGVYHEVVITDAGHSPH
                     VEQPQAFMAALVEFLENWMK"
     gene            complement(13841..15385)
                     /locus_tag="PLC06_04020"
     CDS             complement(13841..15385)
                     /locus_tag="PLC06_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRO89643.1"
                     /translation="MYIGDYLARRELYSPDRLAIIDTGKSPEWRLTYRQMNERANRLA
                     NLLRDEVGVAQGDRVAILAHDGVEHLDAFFACGKLGAVHTALNWRLHPRELADIVART
                     TPRALIFSDDMRSQVAELQALLRGRPHAIGHYFHTEGDGVGGSTHYETTLRSSPATPV
                     TTDGLEAEDIAALIFTGGTTGLPKAAQVSHRMIAWNTLNTVIHDVTHDDVYLNVFPMF
                     HTGGLFVYTLPQVIFGGVTILMRQFDPARVLELIERERATIFAAVPTMYQLITQAPNW
                     ATADLSSLRFCTSGGAPLPVPLVEQYTREKGIRFKQGFGMTEFGPGIFALAPEDAIRK
                     AGSIGRPNFYVDARIVDDDNRFLGPNEDGELVLKGPSYCSGYFHDPVASAEAVDERGY
                     FHTGDIARYDAEGYFYIVDRKKDMFISGGENIYPAEIEKVLYGHPGVLMCAVIGLPDA
                     KWGEVGKACVVLKPGATADEAELLAYMADRLAKYKVPRTVAIMDALPISSAGKILKRE
                     LKTIRN"
BASE COUNT         2655 a         4597 c         5143 g         2982 t
ORIGIN      
        1 ccgctggccg ccaccggccg aagcccggaa gaaatcttct gggaactggc catcgaggac
       61 atccgcaacg cggccgatct gttccggccg ctctttgatg ccacccacgg cggcgacggc
      121 ttcgtcagcc tcgaagtcgg ccccgggcag gccaacgaca ccgaggccac catcgccgag
      181 acccagcggc tgtggtcatt gaccgaccgg cccaacctga tgatcaagat tccggccacg
      241 ctggcgggca tcccggccat caccacggct accgcccacg gcctgaacat caatattacc
      301 ctcatcttcg cccgcgaacg ctacgcggcg gtgatggatg ctttcctgag cgggctggag
      361 cagcgcgtgg cggccgggct gcccatcgac catatcgcct cggtggcctc gtttttcgtc
      421 tcgcgagtcg ataccaatgt cgacaaacga ctggaagcca tcatcggcgc aggcggccca
      481 caggcggccg aagcccgatc attgctgggc aaggcggcca tcgccaacgc caagctggct
      541 tacgccgact tccagcgcgt cttttcatcc gaccggttcg ccgcgctgaa agcgaagggc
      601 gcgcaggtgc agcgaccgct gtgggcgtcc accagcacga agaacccggc ctatcgggac
      661 gtcatctacg tcgaggacct gatcggcccc gatacggtca acaccgtgcc gcaaaagacg
      721 ctgaccgccc tgctggatca tgccgtcctt gcgcaaacgc tgacgaccgg cgtggacgag
      781 gcccagacag tcatcgaccg gctggcggcg ctgggcatcg acatggccga cgtcacccac
      841 gagctggaga tcgagggcgt gcaggcgttc accgatgcct tccacacgct gctggccagc
      901 attgagaaga aattggcggt agtggcggcg tagcgattcg tcgcctgccc caacacgaag
      961 gacggccnnn nnnnnnngcg gcgattattt tacgccgcgg cggccgtttc ctattatccc
     1021 cccgcctgtc gggcagcttc catcagccgc tcccggtcgg caatcctcaa cttcgtgcgg
     1081 gtgatatcga tcaggccttc gtcggccagc tcatagagca gccggcagac gatttcccgc
     1141 ttggtgccga tgagggcggc catctcatcc agcgttcggc cgcgcgaaac atactccccc
     1201 gcggtgtcac catacaggtc gagcaacatg cgcgccagcc gcctcttcac cggctggaat
     1261 gccagttcct ccaggaaatc gccggcctgc aggatgcgcc gggccatcat ggttgacagc
     1321 tcccacatgg cgcggccatc ctcctgtagc aggggctgca ggtaatctcg cggccaaagg
     1381 tacacgtctg tcgggcgttg agcttccagc agcataggca tcggcgcgaa ctcaacgaaa
     1441 aaggccgccc cccaatacag gtcgcccggc ttcatctgca ggacgatcaa actgcgccct
     1501 tccggcgact ttttcaaggc gtggatagcg ccggaaccga ccaggaaaag atagtcccac
     1561 gtctccccat agggcgcgat ccattcccct ttctcgaacc gcttgtgaat tgcctgccgg
     1621 gccaattccg cgcgcgcgga cttatccaga acgccaaaga ccgggctgcg ggccagccaa
     1681 tttgagataa tggacatcat tttccccagt tcggtttttc cgttgattta gctccacaaa
     1741 tcggctccat cttgcctcac tatttgacaa aaagcaaata ccactaccat cgcttgtgat
     1801 ataattcaca aataaaggag aacctcccga aagggggagt agccaaatga atgcattaac
     1861 aaaacgaatc gattatctct ttcggtctac cgcgggatta atcctggtag ccattgccgt
     1921 ggtctcattg atcacggcca tctggggcac gctctcaggc ccaatggtgg aatggggcat
     1981 tcgggacgtg acggtccggc tgttggggat gcagttggtg caagcggatc gtgaggcgcg
     2041 catcatcatg ctctaccaca ccatcgccat gaccatcgta gccatcgagg tctacatcat
     2101 caccgacatc ctgccgatga agcgccacga gcaggtcgtc atcaatgcca ccatcaccat
     2161 cggttacctg acatctgtca tcttcggcat gttgttcgcc tattgggggc agaacttcct
     2221 gttccacggg cttttcctgc tgggccaatc gctggtcttc ttcgccggga ttttgctggc
     2281 ggcggcgttg tggccgtgga agaaggcgta ccggctggcc gcggattctc cctacgcgca
     2341 cacaagaggc ggcgtcgatc tggagcgggt cgccttcttc gcgatggcca tcgccacgct
     2401 gctctcggcc gccttcgggg ccgtcaccgg ttcctattgg ggcaacgggc acgagacgtt
     2461 cctggccgaa gacctcatcc gcgcgccgct caagaccggc ttgcagcggg cgatcatcgg
     2521 ccacttgcat atcatgctga ccctgatcgc catcgccatt actctcatcg tcggccgctg
     2581 gatggatttc aagggccgtc tgcacaaggt cgccatgatc ctgatgatca tcggcacgat
     2641 tatcatcacc tttggggcgt tgtcggtggt ttgggtcgag tgggcgcacg tgaccatcta
     2701 cgtcggttcg gtcttcgtca tgctggccgc gctgctcttc gtcatctact cgtggcggaa
     2761 gctgatccat gaccgcacgg ccgagctggg gttggtcaaa cccacattgg gacagaaggt
     2821 caaagcgctg ctccacgacc cgctcaagtt tggccccggc tggcagatgg tcttcatgaa
     2881 cttcaccgtg agcggcatcg gcatcttcat ggccgtcaag ctggacgaga tattccgggt
     2941 ttggccccac cgcgaggaac gcatcactct gacgggccac tggcacatcc tggccagcct
     3001 gatcgccaca atcatcctga tgtattttgc cgatcgcgcc ggcctcaagg ggcgggcgcg
     3061 caagtggttc ggctggacgc tcatcatcat gtccaacctg gcctttgccg ccgtcaccgt
     3121 cttctcgatg aagcgcctgt ttgtcagcga aggggcgcaa cagccgatcg tcaactggac
     3181 gatgctcctg gccgatattg ggctggccac agtcctaatc gcgctgggga tattcatggc
     3241 ctggcgactc atcgacctgt tccgcgcccg cggccgatgg acgaaggagt tgagagagga
     3301 acgggagcgc gtgacgcgcg cagagattgc cgaaaagcag cggcggctgg aggagctaaa
     3361 ccaaaccctg gaagaggtat ccacgccccg aggaggtgcg gcatgagaac catcatcttc
     3421 cttctgatac tacttctgtc ggtgaccgca tgcggccccg cgcccaacat cgccgccatc
     3481 accccgccct acatcgaaac cggcatcgac gccgacgcgt gggccacagt cccggccggt
     3541 gatttcccct acggccagca cgatcacatg acgccggtcg attacgatta cgagatcatg
     3601 gtcacgttag tgaccaacga acagttcgcc gcttttctga atgaagcatt ggccgccgac
     3661 gttgcgagcc tgggcaccgt ggaggtcgaa gcaggcgagt cggtctggac tgaggaaggg
     3721 gtctttggcc cctatccggg cgatccgttc gacgcctata agcatgagga agagattcgc
     3781 gccggcgaca agctgtggat gtcgacaacg gccgagggag cgcgaatcac gttcgatggg
     3841 caggcgttcg tcgcccagga cgcctatcgt aatcatccgg cgacgatggt cacctggtat
     3901 ggggccaagg cttattgcga gttctacggc gggcgactgc cgactgaaat cgaatgggag
     3961 aagtcggccc ggggtaccga gaccatcgac ggacgcgggc gccctttccc ctggggcgag
     4021 acgatcgaac gcaacaacgc caacttctat tcttcgttcg acctgttcgt gaagatgttc
     4081 ggcaagctgg gcaacactac gccggtcggg ttctataatg gccgcaccta cgacggctac
     4141 cagacgctcg attcggccag cccctacggc ctctatgaca tggcgggtaa cgtcgcccaa
     4201 tggacaggcg acgattaccc gtatcagcat tatcgttatc tgcgcggcgg cagcttctac
     4261 tcgtatgagg tcgatttgag aacgtggaaa tccaacagcg ccggcccgca gtacttcggc
     4321 ccggcggtcg gtttccgctg cgcccgagac agcaatccat aaggcattga gagaacccat
     4381 ggccacgaat ttgcgagcca cgatttcccg atataccccg ctcttcaaga gcttgcagac
     4441 aggtttgttg ctgctgaccg gtctggcggg ctacatgagc gcccgctgcc ccatcttcaa
     4501 cgtgttcacc gtgctggcgg tagcgggcag cctgcttctg gccatcagcg gcagcactat
     4561 cctcaacatg tggtatgacc gggatataga cgcccgaatg gagcgcaccg ccgggcggcc
     4621 gatcccgtcc gggcaggtca gccccgtcga agcgttgcgc ctgggcctgc tgctgtccct
     4681 ggccggcgtg gtctgggccg tgaccctgga tccgctctat ggcgtgatcg tcttcgccgg
     4741 gctgttcttc gatgtcgtcg tctataccat ctggctgaag cggcgcacgc cgtggagcat
     4801 cgtctggggc gggctggccg ggggcatgcc aattctggcc gggcgggcac tgggcatggg
     4861 gcaggtcgaa tgggtcggcg tcgcgctggc gctggccgtc ctgttctgga ttccgaccca
     4921 tatcctgacc tttgccgcgc gtcatcacga ggattaccgg cgggccggcg tgcccacggt
     4981 cccggccgtc tatggcttcg ccatcaaccg ggccatcatc gccgtctcca gtatcctggc
     5041 ggcggtgatg atgggcatcg ccgcctacgg aattggcctg gaggtcggct acctgcgggt
     5101 gatggtcgtg ctctcgatcg gcttgctgac gctggcgatc agaagcctga tgcggccgtc
     5161 ggagcggatc aatttcggcc tgttcaagta tgcatcgctc tacatgctgg ggacgatgct
     5221 gctcatgatg ttcgttcaat gagtggatca atgggtaatc ccgtgaagaa gatgtccgcg
     5281 atcgatcgtg tttttctcct gctcacaggg ctgctggccg cctaccagat cgccatcggc
     5341 gtggaaggcg tgccggcggt gtccctttgg gcctatacgg tcgggttcgg catcctgctg
     5401 ctggccggga tcatgctgat cattctcgga tttgaaggcc tcgacagtcc gtttgtagct
     5461 atcgtgagta cggcggcgcc tctggcgttg tccctgggtc ttgtgtggga ttacctgccg
     5521 gcctggcgag ttgcctatct cgtgtttgtc attgtcgggt ttatcgcggt gacgataacg
     5581 cgtgtggctc ggatgggccg ggtggcgacg atcacgctgg ctgtcgttca cggcgtggcc
     5641 gggttgatca tcttcggact gccaatctac ctgagcctga ccggcacgac gccgggcggc
     5701 ttcatcctgg ttggaatcgg cggggcgctc atcggcgtgg gcgggctgat gctctcattt
     5761 ctgaaaatgg gcaagcctgt cctgtcgcag agcgctatcc tgaccatcct tccgggcctt
     5821 ttatttctca tgacggccgc gtttgtggcc ggtttcacat tttcctgaat ggaggaacct
     5881 ctaatgttcg gcaagatttt gcgagttgtt ggaataattt tgatggcgtt gacagcggtg
     5941 atgacgctgc tgggcggcat cggcacgact tgcgttgccc tcgacgccgc caaatatggg
     6001 gacagcatgg ccgcgttggc tgattatcaa tggctctaca tgctgtatgt cctggccggt
     6061 atcgtgctgg ccatcgccgg tcttcacgcc acgcggctgc tcatcaaaag ccgcgccaac
     6121 gcctaccgca tggccatgat cgtgctggtg tcggggatgg ttgtggggat tatccacatg
     6181 ctcacgtccc gagcgctgcg cggcagctcc atgccggttg atttcatcgt ctatataacg
     6241 gcgttcacgc tggttgtctt cctgctgttc cgaatcccgg gcatctggca gaaggttaat
     6301 ctgacgggca aggacgatca caccaccggc atgggagcgg gcgtggccat gatcgtggcc
     6361 ggcttcatca tcctgactgt gcaggtgtgg ggcggcgcga tgcacaccat caatggcgtc
     6421 aactacgccg acgtgtggca tacccagttg gctttggccg gttcggcggt gctgctggcc
     6481 ggcagtatcg tcctgctgcg caaggtgctg ggcgtgtcgt tgcccaggcc ctcgcaggtg
     6541 acggtggcca aggaagtgat tcgctagacg ccatagcggg cgaacaaaca cacgggggcc
     6601 gggctgcgcg cctgtcctcc tggcgtggtg acagacgatt cagggcgcac acacgaaggc
     6661 aaacttcgca tgtgcgccct gcttgttatt gatgacgacg acgtaatgcc gattagtgcc
     6721 ctagccgttc gtcacgccgc gatgtgttga agatgccggt cggcgtgtcg aggccatcca
     6781 gcacggccac ccgcgcgaag gtttcggcgt cgtagacccg gatgacttca tcttgccagt
     6841 cggcgatgta gacgtagcgg ccgtcgtttg tcatttccgg gtgcagcggt tgtgtgcctt
     6901 cctcgatgac atgaaccacc gcgtggctcg ccttgtcaat gacgtagatg cggttgggca
     6961 cggccgcgaa catcgtgtcg gcccatacat agggggtctg atcgtgggtg cggatgaaca
     7021 ggcccggccc gctggtgggt attttggcga cgatctcgtt atcggccaga tcccagaccg
     7081 tgaccaagcc ctcgcctgcg tgagtcgtcg ccccatactc ggcgccgttg gcttcccacg
     7141 tcgccccgga gccgggatgc ggcgtgtcgc cggtgttgat gcgcgcgacg accttccagt
     7201 tctcgacgtc gatcaccacc atctgattgt ctccctgaga ggcgatgtag aaccgtttgt
     7261 tatcgggact gaggaagccg tcgtggagaa tgcgcccgac gtcggccagc tttgtgacgg
     7321 ggaagtcggg ttggctgtag tcgatgcgcc acatctgccc ggcttctttc agggcgatga
     7381 tgaagtatgg cccgaccagc tcgggggcca catcgctggt gatggccacc cgcgatttca
     7441 cccactcgcc gtccagccca tagccctcgg tatgcagcaa cttgagcggt tgcaatgtgg
     7501 cggcgtccag gatgacggcc gtcgccgggg cgaagttgcc ggcgatcacg tatttgccgt
     7561 cgtcggatat ggccaggccg cgcgaatcga ggccgacccg aatccggctg accggctgca
     7621 gtgagtagag gtcgatcttg tggagccagc cgtcgcggcc gatgttgaag gcccagcgct
     7681 cgttggccgg gtcgaaggcg tagccatgcg cccgatagcc ggagtccacg cgaccgagca
     7741 gcgtatgggt gtcgccgtcg aacacggcga ttcgctgcgc ctcgcgttcg gtgaccagca
     7801 tcagattgtc catgttgccg gtgtgggtag gcacgggtgg cagggtggac tcctcgacca
     7861 attgttcgcg gctggcgatg atgtcgtcca tctcccaggt cgcctcgctc tcgtcgacgg
     7921 ggctgcgcag gaagcccagc atcagccaga tttcggcgtc ggtgaaggtg tatttccagt
     7981 tgggcatggg cgtgcccggg cggccgttgc gaatggtgtc gatgtagaag ctgtcgctcg
     8041 cggtcaggct ctccggcacc agagccgggc cgatggcccc ctggcgatcc gctccatggc
     8101 aggcgccgca ggaatcgcga taggcttcgc ccacggtgcg gccgtcgaag aagtcggccg
     8161 cgccgttcgg cgacacgaca atagcacctt ccagtccggc cgcaccgccg gcggtatagg
     8221 ggtacacgcc gctgccggtg ggcgcccatt ggtagcggcc gccgggcgcg atggccggcg
     8281 agacgaaggc atcattggcg gcggtcaggg tttgggcgct gccggacaga ttggcccact
     8341 ccacgatctc gccgccggcg acggccagaa catggggccg gaagccgtcc gcctcgatgg
     8401 cgatgtcgta gccgacggcc gacagcacga ccggcaacag aatccgggac gcgccgtccc
     8461 tggcccccaa cgcaacacgg ccgcccaggg cggttagtcc ggcgacgcaa agcaatacaa
     8521 gcgcaatgtg aaccttgcgg atcatgatag ctctccccta ttgaacggta ttgatggttc
     8581 cggtgaaccc aattcggggg gattatagcc tgagaaccgg atttctcccc ccaaaatcct
     8641 tggaaaccgg gttttcagga aaaagcccgg tttcgatcat taactcctga aatctttctc
     8701 tccggcgcgg atgggcacag ccagccagtt ctcgcgcggc ggcagggggc aggagaagta
     8761 ggaggaatag gcacaatacg ggttgtaggc gtagttgaaa tcgacctcga tctcgtcctc
     8821 gcccagacgg cgcagccccg gtcggtggtt gtccagataa cgacccgcgc cgtaggtctc
     8881 gttgcccgat gtggcgtcgc ggaagggcag gaagagttct tcgcccgtcg ggtcgctgta
     8941 gagggtcagg cgagcgggct gcgcgtcgac ctcgaaggca aacgtccccc agcgacgaaa
     9001 cggccgcgcg tcgccggtgc tggtctccat ctcgatgatc ggctcgtcgt cgggcaggtg
     9061 ctcgacggtg gcgacgatga ccagcgcggg gttctcgccg tagtagtcca gtcctttgaa
     9121 gccgtcgcgc tggtcgtcgg cgagcggcga ttggggatgg aactgcatga agtcgtctat
     9181 ctgttcgcgg agtgctttca gttggttgtc cattttctct tttgctgcct aaaggaaccg
     9241 ttgcccgcgc tctattctac cccaggaagc tgatgacggc ggcgttggcg gcggcgggcg
     9301 tctcaaaggg gaagaagtgg ccggcgtggg gaaggatttc cacttgcgcg ccggggatgc
     9361 ggccggccag caggggggcg ttggcgggcg gcacgacttt gtcttcggcg ccgaacagga
     9421 tgagcgtcgg ggcggtgacg gcggtcagac gatgttcgaa ggcagccgct tcggcgatga
     9481 gacccaggcc gatggccagc tgggcctgat agccggcggg gtcgatggga tgggcgacgc
     9541 ggtagttgac ccactgctcg acgaattcag gattggcctc gacaaagccg ggcgcgcagc
     9601 tgacggcgat gccgcgccgc agccgctcga tgggatcgcc ggacatgttc atcagcacgg
     9661 ccatcgcctc ggctgagatg ggcacgtggc gtggcccgcc gaagttggtg gccgagagga
     9721 tgagcttgtc cacccggtcg gggtagtcga gggcgaatgc ctgggcgatg aagccgccca
     9781 tcgagtggcc catgatgtgg gctttgggca ggtcgagggc atcgagcaga cccgcgacgt
     9841 cggcggccag catggcggcg gtgtaggggc cgggtggctt gtcgctttgg cccacgccgc
     9901 ggttgtcgat gcggatgacc tgatagcgct cggccaggcc ggggatcatc cggtgccaca
     9961 tccagccgtc gtagccgaga ccggggatga gcaccagtgg ctcaccgcgg ccgtggagtt
    10021 cgtaatacag gttgatgttg ttggtttggg cgtagggcat ggggttgatc cttttttagt
    10081 gacgagtgac gagttacgaa ttacgagtga agagggccga cgacaggcca cagacgacca
    10141 ttattgccgc gtgataggta ggggcgggac agggcggccg aatggtttgg tcttgcgaat
    10201 ggtgtggctg ccgccctgtc tcgccctttt gttgttgtag gcggggaaca gcgctgaagc
    10261 gcttaactac gagcgaagag gggcgagaca accggcgagc gacgtattgg tgattggatg
    10321 aagccatgcg ccggttgtcc cgcccgtaca tataattcca cggcacgctc ttcattcgtc
    10381 actcgtcact tctcgcgtat ttctcgcgca gttcggcttt gatgaccttg ccgtaggggg
    10441 agtaagggag cgactcggcg aagatgactt gcttgggcac cttgtagcgg gccaggcggt
    10501 cgcggcagaa ggcgagcagc tcgccggccg tggtcgcggt ctcgggccgg gccacgacga
    10561 tcatcacgcc cacctcgccc cacttctcgt cggggtggcc gatgagcgcc gcctcgcgca
    10621 cggccggatg ctccaggaag acggtctcca cttcggcggc gtagacgttc tcgccgccgc
    10681 tgatgatcat gtccttgtag cgcccggcca tatagtagaa gccgtcggcg tcctggcggg
    10741 ccatgtcgcc ggtgtggaac cagccgtcgc gcagcgccct ggccgtggct tcggggttgc
    10801 gccaataacc ggagcagaca tgagggccat agatgatcag ttcgcccgtc tcgccgcgcg
    10861 ccacgtcgcg gccgtcctcg tccacgatgc gcatccggct gtggaaaacc ggcttgccga
    10921 ccgaccccac cttggccacc gactcctcgt tggtcatggt gaagcagttg acgccgacct
    10981 cggtcagacc atagccctgg cgcagatagc cgcccttggc cgcgcgccag gcggcgatga
    11041 gcgacattgg gcagggcgcg ccgccgctga cgaagtagcg cacggcgctg aagtcggccg
    11101 cggtaaacga cgggctgcct agccacatct ggaacagcgt gggcacgccc agcacgacgg
    11161 tggcccgctc ccgctggatg agcgccagcg aggcctcgct atcgaacgag cgggccagca
    11221 atatcttccc gcccacgtaa aagagcggcg tcagaaaaat gaacagcccc ccggcgtgga
    11281 acatgggcgt catcaccggc gagatgtcgt cggcggtcag ctcccaactg atgacggtgt
    11341 tgatgcaatt ccacagcact tgccggtggg agatcatcgc tccttttggc cggccggtgg
    11401 tgcccgacgt gtagaggatg cagcagatgt cttcgccggc cggcgggggc gggggtggtt
    11461 cggcgacggg ggcggcggcg acacaggcct cataggccaa cgcgccttcg atggccgcgc
    11521 cttcgatgcc gacgacgacg gggaaggcca cctcgcggcg caattccgcg agcaagctgg
    11581 tgtgttcggg gccgcagatg agaaccttcg gcgcgcaatc ggcggcgatc caggccagct
    11641 cgcgggcggt caggcgccag ttgagcgggg ccagaatcgc gccaatcttg cccaggccat
    11701 agagcagatc gacggtgtgg acgctgttct gggccaggat ggagacgcgg tcgcccggcc
    11761 cgacgccgag gccgcgcagc cagttggcca gccgattggc gcgatcgttg agctgccggt
    11821 aggtgtattc gacgccggtg tggacgtcga gcagagccac ccgatcaggg gtcagttcgg
    11881 cccgcgcgcg cagcaaatcg gccgcgcctt gcggataatg attctcagat gtcatggccg
    11941 cgctccggca agatcaggtg ccgacgacga tgccgccgtc gacgcgcagg acgtcgccgg
    12001 agatgtagct cgcttcgtcc gaggcgagaa acaggtaggc gttggcgatg tcgcgcggct
    12061 cgccgaggcg gccgagcggg acgtgggcct tcatgcccgc caggatgttc tccggcatgg
    12121 cggctaccat ctcggtggac gtgaagccgg gggcgacggc gttgacgcgg atgccatacc
    12181 ggcccaattc gcgcgaccag actttggtca tgccgatgac ggccgatttg gtggcgacgt
    12241 agttggtctg gccgaagttg ccgtcgaggc cgacgatgga cgaggcgttg aggatgacgc
    12301 cgctcatctg gcggatcatg tggggggcga cggcctgggt gcagttgaac acgcccttga
    12361 ggttgacgga gatgacgagg tcgaaatcgg cttcggacat ctgtttgacg aggacgccgt
    12421 ctttcatctt gaccagggtg ccgtcgcgca gcacaccggc gttgttgacg aggacgtcga
    12481 cgcggccgta ttgttcgacg acttcatcga cgaatgcgcc gacggcggcg cggtcggtga
    12541 cgttgacctt gacgaagcgg gcggccgggc cgagttcttc ggccgtcttg tggccctgtt
    12601 cctcgttcac gtcacagata atgacggacg cgccttcctc aatgaagcgc tgggcggtgg
    12661 cgcggccgat gcctgctgct ccaccggtga tgatgcagat tttgaatttt aagcgcatga
    12721 ggtctcctta attacgaagt acggactact tcatttcatc caattttcca aaaactcgac
    12781 cagcgcggcc atgaatgcct gcggctgctc gacgtggggg ctgtggccgg cgtcggtgat
    12841 gacgacttcg tggtagacgc cgccctgggc cacgtagcgg cgcaagacgg tgcgggtctg
    12901 caggagcatg ggctggggcg ggaacacgtc gtcgcccggc caaccgggaa ccgcgccaag
    12961 cttgcccagc gtgccgaagt cgaacaggga catatcaccc acgatctggt cgctgtcgcc
    13021 gcgaatccaa aggatcggcg gcttgtgggg catgtcgacg atgccgctca ggttcaggtg
    13081 cctggggctg gtggcgttga tggggccgta gacgccaggg gcgacattgg gccagttgac
    13141 cgagggggtc aggtcgccgg ggtagcggtc gttgccggtc ttctccgaca gcagcgaact
    13201 gaggaactct tcctcgcggg cggcgcggaa gggtggcttg tagtagaagg cgttgatgac
    13261 gttgcgcggg ctgttggggt cgtcgccgga gcgatcgtgg atctgcatgc ggcggacgaa
    13321 ctccgggttg acgctgccgc cgccggaacc ggcgcagtcg tcgtagcagg ggacgccgtc
    13381 ctcgcccttg gtgccgccga agccgtaggg gctgatgggg ttgacgaggg tgagcgattt
    13441 caccagcgcg gggttgtcga tggcgaactg gagaatcggc gcggccccca gtgaccagcc
    13501 gacgaggtgg gcggggcgct cgcccagggc gtcggacagc gcgcgcaagt cgtccgacca
    13561 gtcctttgcg ccacgagtcg cgtcgacgag caggtcttca gtgtcgccgt agccgcgcag
    13621 gtcgggggcg atgccgcggt attgggcggg cagggcgagc atgctctcct cccagaaggt
    13681 ggctgatgag gcattgccgt ggacgaaaac gaccggttgg ccgtcgtcgg ggccgctgaa
    13741 caggacgtgg gtgttaatgc gggcggtagc taccatccgc gaggtgatgc cgggaaggat
    13801 gggaatgggt gacatggttc tccttgctaa ttcgtaattc ctaattccta attgtcttca
    13861 gttctcgttt caggattttg ccggcggacg agatgggcaa tgcgtccatg atcgccaccg
    13921 tccggggcac cttgtatttt gccagacgat cggccatgta ggccagtaat tcggcttcgt
    13981 cggccgtcgc gccgggcttc agcaccacgc aggccttacc cacttcgccc catttggcgt
    14041 cgggcaggcc gatgacggcg cacatgagca cgccgggatg cccatagagc accttctcga
    14101 tttcggccgg gtagatgttc tcgccgccgc tgatgaacat gtctttcttg cggtcgacga
    14161 tgtagaaata gccttcggcg tcgtagcggg cgatgtcgcc ggtgtggaag tagccgcgct
    14221 cgtcgacggc ctccgcgctc gccacggggt cgtggaagta gccggagcag tagctcggcc
    14281 ctttcagcac cagctcgccg tcttcatttg gccccagaaa gcggttgtcg tcatcgacga
    14341 tgcgggcatc gacgtaaaag ttggggcgac cgatgctgcc cgccttgcgg atggcgtctt
    14401 cgggagccag ggcgaagata cccggcccga actcggtcat gccgaagccc tgcttgaagc
    14461 ggatgccctt ctcgcgggtg tattgctcca ccagcggcac gggcagcggc gcgccgccgg
    14521 acgtgcagaa gcgcagcgac gacaggtcgg ccgtggccca gttgggggcc tgggtgatga
    14581 gctggtacat ggtgggcacg gcggcgaaga tggtggcgcg ctcgcgctcg atcagctcca
    14641 gcacccgcgc ggggtcgaac tggcgcatga ggatggtgac gccgccaaag atgacttgcg
    14701 gcagggtata gacgaacagg ccgccggtgt ggaacatagg gaagacgttg aggtagacat
    14761 cgtcgtgggt cacgtcgtgg atgacggtgt tgagcgtgtt ccaggcgatc atgcggtggg
    14821 agacctgggc ggctttgggc aggccggtgg tgccgccggt gaagatgagg gcggcaatgt
    14881 cttcggcttc gaggccgtcg gtcgtcacgg gcgtggccgg cgaggagcgc agggtggtct
    14941 cgtaatgagt gcttccaccg acgccgtcac cctcggtatg gaagtagtgg ccgatggcat
    15001 gggggcggcc gcgcaagagg gcttgcagtt cggcgacctg ggagcgcatg tcgtcggaga
    15061 agataagcgc gcgtggggtg gtgcgggcca cgatgtcggc cagctcgcgg ggatggagcc
    15121 gccaattgag ggcggtgtgg acggccccca gcttgccgca ggcgaagaag gcatcgagat
    15181 gctccacgcc gtcgtgggcc agaatcgcca cccggtcgcc ctgggccacg ccgacctcat
    15241 cgcgcagcag gttggccagc cggttggcgc gctcgttcat ctgccggtag gtgagccgcc
    15301 actcaggcga cttgccggtg tcgatgatgg cgaggcggtc gggcgaatag agttcgcggc
    15361 gggcgaggta gtcgccgatg tacatga
//
LOCUS       DAOMQG010000040        15047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1714_length_15047_cov_9.379869, whole genome shotgun sequence.
ACCESSION   DAOMQG010000040 DAOMQG010000000
VERSION     DAOMQG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1714_length_15047_cov_9.379869"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PLC06_04025"
     CDS             complement(<1..1335)
                     /locus_tag="PLC06_04025"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89644.1"
                     /translation="MQENVKALSPDKLRLVCDPTGLPFESTIDLEPTTAIIGQPRATK
                     ALEFGMGLKSKGYNIFVTGSTGTGRSTAIRHFLQERCSQEPTPNDLLYLYNFEANHRP
                     QVVHLPPGEGNIFAGQVETLIEAAQREFTQALESSSYRDAVHALEQKVHEQRETRLDA
                     LERKAAEQGFDLQDTPAGLAILVADEEENGEPNNANGEQQARRETQRALQTELQDILR
                     EVRSLERDAREERRGLDKRVAEAAVLDEFDRLREQYVDEPTIHTYLHAAREDLIDQVA
                     RNAPGLDEKDPDQFIDLRRYEVNILVDNVVGKGAPVVVQLNPTYENLFGRLEYETQGH
                     AMTTHFTQIRAGDLHRANGGYLVMYALDLIRQRETWEALKRALKAQEIETRPQSSDGP
                     IIANSLWPQPVPLDVKIILLGSSGVYDSLYWNDEEFSDLFKVRADFSDTMPRD"
     gene            1512..2939
                     /locus_tag="PLC06_04030"
     CDS             1512..2939
                     /locus_tag="PLC06_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO89645.1"
                     /translation="MASTNTGDSTSLSTATKFIFGMGDWGTSAASTARNVFWFVFLTN
                     VVGLHVGLVGGIWLVGRLWDAINDPLVGSLSDRLHSRWGRRRPFLLLGSVPFALTFFL
                     MFIVPPIESKGWLLFYYGAVFLLYDTLFTIVNVPYLALVPELADSYDERSSLTGWRTA
                     FSFLAQLVTAGAFKLLAENVFAPWFGGGPEGIRAGYLLAAGLWAVSMALPYIILALTL
                     REPDHEPVSSPIEPWRNFRDVFSNRPFRLAALIYLLCFTTGDLLLLVFVRYLIDYVQM
                     RPGLDNLVLTVVLGTSLLSIPLVLALMRRTDKRTAYLISIGFMIIVLALGSFLPPGGQ
                     NLIFIGAVLAGVGFAAMTIVPWAIVADVIEVDELQSGERREGLYTGYLVFLRKLATGI
                     VVFIAGQVLAATGFLSTTTGSQFIQQPAAALNAMRFMVTVVPAISLALSWILAWRFPL
                     DRDAYETIRHELEARRAQQTTPTAP"
     gene            2970..3437
                     /gene="bcp"
                     /locus_tag="PLC06_04035"
     CDS             2970..3437
                     /gene="bcp"
                     /locus_tag="PLC06_04035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRO89646.1"
                     /translation="MLQIGDPVPDFELLTDTGEPLKLSDLRGRRVALFFFPKADTPGC
                     TKQACGFRDHFPYIETANATVLGLSPDQPKALAKWKKKERLPYTLLSDPEHEVAEAFG
                     AWGEKSMFGKAYMGILRGHAIIGPDGTIEDLRLKISPDESVEEAMKFINAAAN"
     gene            3447..4427
                     /locus_tag="PLC06_04040"
     CDS             3447..4427
                     /locus_tag="PLC06_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRO89647.1"
                     /translation="MRAIRFDRFGPPAEVLYLTNLPVPEPGPGQVRLRLTHRPINPSD
                     LLTVSGEYGSLPRLPATPGFEGVGHVAALGEGTRGLAPGMRVVPLSAGGTWRESAVVE
                     AAMLVPVPDAVDDAAAAQFVVNPVTAWVMLEEELKLQPGDWLVQTAAGSTLGRLVIQL
                     GRLRGYRTINLVRRPEQIDELLALGADAAFATGEDDIVERVRELTGGRGAAAALDAVG
                     GETGALALRSLRPGGTLLIYGMLGREPLPLHNGEMLFRGLAVRGFWLTHWFRKSPPMQ
                     VAATLGNLMQLMAQGDLVPPVEAEYDLADFRAAIAHAERPGRRGKILLTG"
     gene            complement(4471..>6840)
                     /locus_tag="PLC06_04045"
     CDS             complement(4471..>6840)
                     /locus_tag="PLC06_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89648.1"
                     /translation="GPVTEGYFHFSNHFRGLDLLQTGLLFNYNPDGGAAFRLGLAQVG
                     LAIAGAAAWLLRKGTTDHRPQTTGHEQSKATGFLHSSFVTRHSSFVILSALIAVFMIT
                     PLSRPLWDHLPLLPFTQFPWRFLSVASFFLALLSGGLVPATDRPAGRAAIAVTGSLVL
                     LAAGLLGLRVDHLILTDADVTAERLAQYEWFTGNIGTTVSGEYLTPESSPRPWASAWL
                     NAAERDRVVALSGELSAELIDRRAARQTWRLVAGEGGAEARFPTMQWPGWRATLDGQA
                     ATLRAVPSSGLMALTVPAGEHTVTLALGPTPVRRAAELVSLLAAVVVVGLAVIDRRRP
                     TGGRQRATLWSVVCSLLLFLALAILWRNDPAELPRGDLTWDFAQMGYLHHTPGGVPFG
                     DGSKLHEYGYSGDVITAGGTLTVTLSITAGDAGTATLALVTPAVARPVPDGAAEPPTI
                     AAQTIGLDRETAVFTLPIPSNAPAGLYVPRLVLDGARSLTPSGATRGDLFLRPIRLVA
                     NDAPLTTPNPAIDAEAVDIALRDASTLDGRFRWQTAAPFAGRYQVSWRAQNGDGAVLS
                     QLDAQPGYGFNPTDGWAADGTVFDWLALRLPAALAGPPPYPLVMVLYDVTTGEQLLAR
                     RTGRLVESGGRIIYQPDSPVFAPPDGLEPFEATFGNDGSEFIRLLGFEAAQTGSVLEL
                     TLYWQAMGSIPADFTRFVHLIDASGAPVAQVDGRPVANSYPTDQWTDGEVVADTVRID
                     LSILPSGEYRLATGFYRPAGDLSRLDAATPDGPLPDGRAVLPWKYRLVE"
     assembly_gap    6841..6940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6941..7760)
                     /locus_tag="PLC06_04050"
     CDS             complement(<6941..7760)
                     /locus_tag="PLC06_04050"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HRO89649.1"
                     /translation="MSNREPLAVRHFSAASSRLSGSDLPWIAAVLLFALVAAWPAISQ
                     PGLLNTRGGGDSPFLLQRLHQLATALADGHFPVRWMPDANYGYGYPFYNYYAPLSIYI
                     AAAFRFLGFSFVRAIHLAQLAGFAVAGLGMFYLGRRWLGSRWAGLLAAVAYTVAPFHM
                     VNVYTRGDSLAEFWAMAFYPLILLAADGLFGEGEKGRGGERGKERGGERGKGRKGEGE
                     KEHPFTPSPLRPRPIALFALAYAALILSHNISALIFSPFLVLYLAIRLFAARQKS"
     gene            complement(7791..9443)
                     /gene="pgm"
                     /locus_tag="PLC06_04055"
     CDS             complement(7791..9443)
                     /gene="pgm"
                     /locus_tag="PLC06_04055"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HRO89650.1"
                     /translation="MTLHPLAGKPAPPEMLVNIPRLVAAYYSNHPDVADPAQRVTFGT
                     SGHRGTSTDGSFNEDHILAICQAISEYRAGQGITGPLFAGMDTHALSEPALSTAIEVF
                     AANGVNLIVQRGRGYTPTPVVSHAILTWNRDSAPARADGVVITPSHNPPEDGGFKYNP
                     PEGGPADTGTTKWIQDRANELLAAGLRGVKRLTLAQAMAASTTREYDYIAPYVDDLGA
                     VIDMDAIAGAGLKIGVDPMGGAGVAFWQPIADRYGLTLDIVNPYVDPTFRFMTVDHDG
                     KIRMDCSSPYAMTGLIRLKDDFDIAFGNDPDFDRHGIVTRSAGLMNPNHYLAVAIGYL
                     FQNRPAWRGDAAVGKTLVSSSMIDRVAASLGRRLAEVPVGFKYFVDGLLSGDYGFGGE
                     ESAGASFLRRDGKVWTTDKDGFIMDLLAAEIMARTGRDPGEHYRELTGRFGDPAYERI
                     DTPVTAARKAVLKNLSPDLVRADTLAGEPILAKLTRAPANNEPIGGLKVVSENGWFAA
                     RPSGTEDIYKVYAESFKGRDHLRRIQAEAETIINDAFAASGV"
     gene            complement(9481..9777)
                     /locus_tag="PLC06_04060"
     CDS             complement(9481..9777)
                     /locus_tag="PLC06_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRO89651.1"
                     /translation="MKIVTQRPRLRRGIGAEDIQDLLEQLELRGEFVTLTTIPPRCRD
                     PKDHPVLATAIDGQADAIITGDDDLRADDELRRAMADYGVELWGVQTLVDTLID"
     gene            complement(9917..10156)
                     /locus_tag="PLC06_04065"
     CDS             complement(9917..10156)
                     /locus_tag="PLC06_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89652.1"
                     /translation="MTATTVTIPNVHLSLDDLIRAVRQLEPEARAQVARALIQDDLDR
                     RLADLIKRLADKQPPVEVTVAEINAEISAVRKQGH"
     gene            complement(10355..11674)
                     /locus_tag="PLC06_04070"
     CDS             complement(10355..11674)
                     /locus_tag="PLC06_04070"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRO89653.1"
                     /translation="MKNKLLLLIPALLLGLTFLLAGRSYAAPIAQSGNLLNNPGFDPP
                     PDAKGAATGWGRWHRETPRSGEECMVAYHYQPKWSVETNPAFVHSGSGAQAVGNSWDT
                     WSGGVYQTVPATPGTVYRFTAYGRGRASNKQVPEPSDSSLQMNMRVGIDPNGSGLWND
                     ADVVWGAAGSPHDTWQQFSVEVTATGNQITVFTSADWGVTGVNQCRAHLDTWFDTGEL
                     VAVSVATATPAPLPTQAPVVATQPPPPTEGVPTEAAAATVIATMTQAPPPETPTPVDT
                     ASICVNAFMDTNGNGLHDPDEGYVAGVTFTVAQGTTIIGQAVSTGMGDPICFSNLAAG
                     TYQVGQTPSPSLEMTTQANASVTVGQGQTVGLEFGSRLRLTPTTGAPEDAAGTAVAET
                     LTATAPAPGNTPSSGSGTPGWLVGLGIAAIAIGVLLLGMLLFMILRR"
     gene            complement(11712..12707)
                     /locus_tag="PLC06_04075"
     CDS             complement(11712..12707)
                     /locus_tag="PLC06_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO89654.1"
                     /translation="MSTISWPIGLIVAGGILLGGLAVPPRQDATATPAAETPIPDSGP
                     TAEPTLPFAPPPWVSPTPDATGAIVVIVQPGESLWVIAARAGLSLPDLLALNGLTEQS
                     IINPGDALIVGYSTPEATIAPEIIAPPTATFPPPTPQPTGKPREAAICLTAFDDLNRN
                     GIHDPGEALRAGVAFTVYNTEAVVANYITDGRSEPRCLGGLVPGNYRVTRSIAPGELL
                     TTGGDWALSLTAGGELYQAFGSVIEEAVAQATSAALQPTMTTAAPPTSVAVQPANNGG
                     SLLLRLFGVGVLFFGGLLLFGAVLILLIRQSRNLPATKPPDEGGERRFQNIDELE"
     gene            complement(12729..14738)
                     /locus_tag="PLC06_04080"
     CDS             complement(12729..14738)
                     /locus_tag="PLC06_04080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89655.1"
                     /translation="MPQSAQYRPSIRRAIRSVRGPSLRGWALLLGVIVLAAGLRLYQL
                     GVWPPGPYRDEAYNGLDALGVLRGGHALFFPANNGREPSYIYLVAISFALLGPTVFAL
                     RLPAAVIGALATLPAYLPARDWFGRATGLFAATLWAITFWPVHLGRIGLRAGLLPPLL
                     ALTFWLGTRAWRETQNDRRATGLWLAAGAVYGLSFYTYLAVRFTPLILALFAVYLVLT
                     GRRERLWSGGRILWFALGAGLVVAPLGIIMLAEPSLIFGRAGQVSILSPTINGGNPLD
                     ALLGSTARAVGMYIWRGDDILRHNAWLSYGTPVPAGRPVFDWLMVVPFLVGLVWCLRH
                     WRRPPAALLLIWQLVMLGPTILAEDAPHFLRAVGVLPGALFFPAIGLGLLWSWPRLPV
                     ALRRTAVVLLLAGSAALTMRDYADYAQQPDVGYLFESAAAELAYSAAADATSGAAVYL
                     DRRFPDGWPSVRFLLDGHRYTPFSPDEGLPPLVGPASVVYAWPYDSLEFLAAVHPPAR
                     VEVAPGPLARGDLEPEPYSLYTRYAIMPGPATNEPVAIFGETFLLHQATTEATAPDTL
                     MVAMDWESAAAGPVPTQFIHILGPEGMLAQYDGPPGRGLWPPDRWQAGIVVGEHHELH
                     LNRPFNPATDSIEIGLYWAETGERLPVLDARGNPAGDAVIVSPAP"
BASE COUNT         2627 a         4640 c         5036 g         2644 t
ORIGIN      
        1 gtcgcggggc atcgtgtcgc tgaagtcggc ccgcaccttg aacagatcgc tgaactcttc
       61 gtcgttccag tagagcgagt cgtagacgcc ggaactgccc agaaggatga tcttgacgtc
      121 cagcgggacg ggctgcggcc acagggagtt ggcgatgatg gggccatcgg aagattgcgg
      181 ccgcgtctcg atctcctggg ctttcagcgc cctcttcagt gcttcccacg tttcccgctg
      241 gcggatcagg tcgagcgcat acatgaccag atagccaccg ttggcgcgat ggagatcacc
      301 ggccctgatc tgggtgaaat gggtcgtcat cgcgtgcccc tgcgtctcat actccaggcg
      361 gccgaagaga ttctcgtagg tcgggttgag ctggaccacc accggcgcgc ccttgccgac
      421 gacgttgtcg acgaggatat tgacctcgta gcgacgcaaa tcgatgaact ggtcggggtc
      481 cttttcgtcc agtcccggcg cgtttcgcgc cacctggtcg atcaggtcct cgcgcgcggc
      541 gtggagatag gtgtggatgg tcggttcgtc cacatattgc tcgcgcagcc tgtcaaactc
      601 gtcaagcacg gcggcctcgg ccaccctttt atccagcccg cggcgttcct cgcgcgcgtc
      661 acgctccagg ctccgcacct cgcgcagaat gtcttgcaat tccgtctgga gcgcgcgctg
      721 ggtctcccgg cgtgcctgct gctcgccgtt ggcgttgttc ggctcgccgt tttcttcttc
      781 gtcggccacc aggatggcca ggccggccgg cgtgtcctgc aggtcgaaac cctgctcggc
      841 cgccttgcgc tccagtgcat ccagccgtgt ctcgcgctgt tcgtgcactt tctgctccag
      901 cgcatggacg gcatcgcgat aggaactgct ctccaatgcc tgggtgaact cgcgttgggc
      961 cgcctcgatg agtgtctcca cttgcccggc aaagatgttg ccttcgccag gcggcagatg
     1021 aacgacttgc ggccggtgat tcgcctcgaa gttgtagaga tagagcagat cgttgggcgt
     1081 cggctcctgg ctgcagcgct cctgcaagaa gtggcggatc gccgtgctgc gtcccgtgcc
     1141 cgtcgagccg gtgacaaaga tgttgtaccc tttgcttttc aaccccatgc cgaactcaag
     1201 cgccttcgtg gcccgcggtt ggccaatgat ggcggtggtc ggttccaggt cgatcgtcga
     1261 ttcaaacggc agaccggtgg ggtcgcatac cagacgaagt ttgtccggtg aaagagcttt
     1321 cacgttttcc tgcataaaac tatcgcttat tacctcgatc catgggggct aacgtcaaat
     1381 catactgcta cttttgagga atggcaaaga atgaagttgg tacgcggccg ttagcgtgat
     1441 agcatttatc gagcgctcac tcaacttctt cgctccggcg aacgacacaa gcgaggcggt
     1501 gatattcatc tatggcctca acaaatacag gcgattcgac gtcactatcc accgccacca
     1561 agttcatctt cgggatgggc gattggggca catcggccgc atcgacggcg cgcaacgtct
     1621 tctggttcgt ctttctgacc aacgtcgtcg gtctgcatgt cgggttggtg ggtggaatct
     1681 ggctggtcgg tcgcttgtgg gacgcaatca acgacccgct cgtgggcagc ctcagcgatc
     1741 gcctgcattc gcgctggggg cggcggcggc cgttcctgct cctcggctcc gtccccttcg
     1801 ccctgacgtt cttcctgatg ttcatcgtgc cgcccatcga aagcaagggg tggctgctct
     1861 tctattacgg cgcggtcttc ctgctctatg acacgctctt cacgatcgtg aatgtgccct
     1921 atctggcgct ggtgcccgaa ctggccgaca gctatgatga gcgcagcagt ctgaccggct
     1981 ggcggacggc cttctcattt ttggcccagt tggtcaccgc cggcgcgttc aagctgctgg
     2041 cggagaatgt gttcgcgccc tggtttggcg gcgggccgga aggcatccgc gcgggctacc
     2101 tgctggccgc cgggctgtgg gccgtcagca tggccctgcc ctatattatc ctggccctga
     2161 cccttcgaga gccggaccat gagccggtgt cttcgcccat cgaaccctgg cgaaattttc
     2221 gcgatgtgtt cagcaatcgg ccgtttcgcc tggcggcgct gatctacctg ctgtgcttca
     2281 ccacgggaga tttgttgctg ctggtgttcg tgcgctatct gatcgactat gtgcagatgc
     2341 ggcccggcct ggataatctc gtcctgactg tcgtgctggg aacttcgttg ctcagcattc
     2401 cgctcgtcct ggccctgatg cgccgcacgg acaagcggac ggcctatctg attagtatcg
     2461 gcttcatgat catcgtcctg gctttgggat cgttcctgcc gccgggcggc caaaacctca
     2521 tttttattgg cgcggtgctg gccggcgtgg ggtttgcagc gatgaccatc gtgccctggg
     2581 cgattgtggc cgacgtcatc gaggtggacg agctgcaatc gggcgagcgg cgcgaaggcc
     2641 tatacaccgg ctatctggtg ttcctgcgca agctggcgac cggcatcgtc gtcttcatcg
     2701 ccgggcaggt gctggcggcc accggctttc tcagcaccac gaccggcagc cagttcatcc
     2761 agcagccggc ggcggcgctg aacgccatgc gcttcatggt gacggtagtg ccggcgatat
     2821 cgctggctct gtcgtggatt ctggcctggc gttttccgct ggatcgtgat gcctatgaaa
     2881 cgatccggca tgagttggag gcgcgccgcg cgcagcagac gacgccgacc gcgccctagt
     2941 cccatcatat caatcacagg agtatttcca tgttgcaaat aggtgatccc gttcctgatt
     3001 ttgaattgct gaccgacacc ggcgagccgc tgaagctgtc ggatctgcgc ggccgtcgtg
     3061 tcgcgctctt cttcttcccc aaggccgaca cgcccggctg caccaagcag gcctgcggct
     3121 tccgcgacca cttcccctac atcgagacgg ccaacgcgac cgtgcttggc ctcagccccg
     3181 accagcccaa agcgctggcc aaatggaaga agaaggagcg cctgccctat accttgctgt
     3241 ccgaccctga gcatgaggtc gccgaggctt tcggcgcatg gggagagaag tcgatgttcg
     3301 gcaaggccta catgggcatt ctgcgtggcc acgccatcat cggtcccgac gggacaatcg
     3361 aggatttgcg cctcaagatc agtcccgacg agagcgtcga ggaagcgatg aagttcatca
     3421 acgcggccgc caactaggga atagtcgtgc gcgcaattcg ctttgatcgt tttggcccgc
     3481 cggccgaagt cctgtatctg accaatttgc ccgtgcccga accggggccg ggtcaggttc
     3541 ggctgcgact gacccatcgg ccgatcaacc cttccgatct gctgaccgtg agcggcgaat
     3601 acggctcatt gccgcgactg ccggccacgc cgggcttcga gggcgtgggg catgtcgcgg
     3661 cattgggcga ggggacgcgc ggcctggcgc cggggatgcg cgtcgtgccc ctcagcgcgg
     3721 gcgggacatg gcgcgaatcg gccgtcgtcg aggcggccat gctcgtgccc gtgcccgacg
     3781 ccgtcgatga tgccgcggcg gcccagttcg tcgtcaaccc ggtcacggcg tgggtgatgc
     3841 tggaagaaga gttgaagctc cagccgggcg attggctcgt gcagacggcc gccggttcca
     3901 cattggggcg gctggtcatt cagctcggcc gcctgcgcgg ctaccggacg atcaacctcg
     3961 tgcggcgacc ggagcagatc gacgagcttc tggcgctggg agccgacgcc gcctttgcca
     4021 ccggggagga cgacatcgtg gagcgcgtcc gggagctgac cggcggccgc ggcgcggcgg
     4081 cggcgctcga cgctgtgggc ggcgaaacgg gcgcgctggc cttgcggtcg ttgcggcccg
     4141 gcggcacgtt gctgatctat ggcatgctcg gccgcgagcc gcttccgctg cacaatggcg
     4201 aaatgctctt tcgcgggctg gcggtgcggg gtttctggct cacccactgg ttccgcaagt
     4261 cgcccccgat gcaggtcgcg gcaaccttgg gaaacctgat gcagctcatg gcgcaaggcg
     4321 atctcgtgcc gccggtagaa gcggagtatg acctggccga ctttcgcgcc gccatcgccc
     4381 acgccgagag gccggggcgt cggggaaaaa tcctgctgac gggttgaccc caaatcggct
     4441 gcagaggcgg attgctctcg ttgtcggccg ctattcgacg agacgatact tccacggcag
     4501 cacggcgcgg ccgtccggca gggggccgtc gggcgtggcg gcgtcgagcc gcgacaagtc
     4561 gcccgccggc cggtagaatc cggtggcgag acgatactct cccgacggca agatcgacag
     4621 gtcaattcgc accgtgtcgg ccacgacttc gccgtccgtc cactggtcgg tcgggtagct
     4681 gttggccacg gggcggccgt ccacctgggc gacgggcgcg cccgaagcat cgatcagatg
     4741 cacgaagcgg gtgaagtcgg ccgggatgct gcccattgct tgccagtata aagttagctc
     4801 cagtacgctg cctgtctgtg ccgcctcaaa tcccaacaat cgaatgaact cactcccgtc
     4861 gttgccgaag gtcgcctcga aaggctccag gccgtcgggc ggcgcgaata cgggcgaatc
     4921 gggttgatag atgattcggc cgccggattc cacgagccgg ccggttcgcc gcgccagcag
     4981 ttgttcgccg gtcgtgacgt cgtagagcac catcaccaac gggtagggcg gcgggccggc
     5041 cagcgcggcg ggcagccgca aggccagcca gtcgaacacg gttccatcgg ccgcccagcc
     5101 gtcggtgggg ttgaatccat agcccggctg cgcgtccagt tggctcagca ccgcgccgtc
     5161 gccgttctgc gcgcgccacg agacctgata gcgcccggcg aacggggcgg ctgtttgcca
     5221 tcggaagcgg ccgtcgagcg tcgacgcgtc gcgcagagcg atgtcgaccg cttccgcatc
     5281 gatagccggg ttcggcgttg tcaaaggcgc atcgttggcg accagccgga tcggccgcag
     5341 aaacaagtcc ccgcgcgtcg cgcccgaagg cgtcagcgac cgtgccccat cgaggacaag
     5401 gcgcgggaca tagaggcccg ccggcgcgtt ggaagggatg ggcagcgtga agacggccgt
     5461 ctctctgtcg agaccgatcg tttgggcggc aattgtcggt ggttcggccg cgccgtcggg
     5521 cacgggccgg gcaacggccg gtgtaacgag ggccagcgtc gccgttccgg catcgcccgc
     5581 cgtgatgctg agcgtcaccg tcagcgtgcc gccggccgtg atgacgtctc cgctgtagcc
     5641 atattcatgg agtttcgacc cgtcgccgaa ggggaccccg ccgggggtgt ggtggagata
     5701 acccatctgc gcgaagtccc aggtgagatc gccgcgaggc aactcggcgg gatcgttgcg
     5761 ccacaggatg gcgagggcga ggaagaggag gagactacag acgacggacc acagggttgc
     5821 ccgttggcgg ccgccggtcg gccgccggcg gtctatcacc gccagcccca ccaccacaac
     5881 ggccgccagc aacgacacca gctcggccgc ccggcgcacg ggcgttgggc caagcgccag
     5941 cgtgaccgta tgctctccgg cgggcacggt cagggccatg agaccggatg acgggacggc
     6001 gcgcaaagtg gccgcctggc cgtcaagggt cgcccgccag ccgggccatt gcatcgtcgg
     6061 gaagcgggct tccgcgccac cctcgcccgc caccagccgc catgtctgac gggcggctct
     6121 gcgatcaatc aattcagctg atagttcacc cgacagcgcc acgacgcgat cgcgttcggc
     6181 cgcgttgagc caggcgcttg cccacggccg cgggctggat tccggcgtca gatattcgcc
     6241 gctgacggtc gtgccgatgt tgccggtgaa ccattcatat tgggccagcc gttcggcggt
     6301 tacgtcggcg tcggtcagga tcagatgatc gacgcgcagg ccgagcagcc cggcggccag
     6361 cagcacgaga ctgccggtga cggcgatggc cgcgcgcccc gccggccggt cagttgccgg
     6421 caccagcccg ccgctcagca aagccaggaa aaaggacgcg accgacagaa agcgccacgg
     6481 aaactgggtg aagggcagca acggcaaatg atcccacagc ggccgggaga gcggggtgat
     6541 catgaagacg gcgatgaggg cggagagaat gacgaatgac gagtgacgag tgacgaatga
     6601 agagtgaaga aacccggttg ctttactttg ctcgtggccc gtggtctgtg gtctgtggtc
     6661 tgtcgtcccc ttccgcagca gccacgccgc cgcgccggca atcgccagtc ccacttgcgc
     6721 cagtccgagg cggaaagccg caccgccgtc ggggttgtag ttgaagagaa gccccgtctg
     6781 cagcagatca aggccgcgaa aatggttgct gaagtggaaa tacccttcgg tcaccggccc
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctcttctg cctcgcggca
     6961 aacagcctga tcgctagata caacaccaga aacggcgaga agatcagcgc cgagatgttg
     7021 tggctgagga taagcgcggc ataggcaagg gcgaagaggg cgatggggcg ggggcgaagg
     7081 ggtgaagggg tgaaagggtg ttctttctcc ccttctccct ttctcccttt ccccctttct
     7141 ccccctctct ctttccccct ttctccccct ctccctttct ccccttctcc aaacagtcca
     7201 tcggccgcca acaggatcaa cggatagaac gccatcgccc agaactcggc cagcgagtcg
     7261 ccgcgggtgt agacgttgac catgtggaac ggggcgacgg tgtaggcgac ggccgccagc
     7321 agcccggccc agcgcgaccc caaccagcgc cgccccagat aaaacatccc cagcccggcg
     7381 acggcgaacc cggccagctg ggctagatgg atggcccgca cgaagctgaa gccgaggaag
     7441 cgaaacgccg cggcgatgta gatggagagg ggcgcgtagt aattatagaa aggatagccg
     7501 tagccatagt tggcgtcggg catccagcgc acggggaaat gaccgtcggc cagcgccgtc
     7561 gccagctggt gcagccgttg cagcaggaag gggctgtcgc caccgccgcg cgtgttcagc
     7621 aggcccggct gcgaaatggc gggccacgcg gcgaccagag cgaacagcag cacggcggcg
     7681 atccacggca gatcggagcc tgagaggcgg gaagaggcgg cgctgaagtg cctgacggcg
     7741 agcggctccc gattactcat gtgggcgctg tggtggcctc aagggccgga tcagacaccc
     7801 gaggccgcga aggcatcgtt gataatcgtc tcagcctcgg cctggatacg ccgcagatgg
     7861 tcgcggccct taaagctctc ggcataaact ttgtagatgt cttccgtgcc gctggggcgg
     7921 gcggcgaacc agccgttctc gctgacgact tttagcccgc cgatgggttc gttgttggcc
     7981 ggggcgcggg tcagcttggc caggatcggc tctccggcca gcgtgtcggc gcgaacgagg
     8041 tcgggcgaga ggttcttcag caccgccttg cgcgcggccg tcaccggcgt gtcgatgcgc
     8101 tcataggccg ggtcgccgaa gcggccggtc agctcgcggt aatgctcgcc ggggtcgcgg
     8161 ccggtgcggg ccatgatctc ggccgccaat agatccatga tgaagccgtc cttgtcggtc
     8221 gtccacacct tcccgtcacg ccgcaggaac gacgctcccg cgctctcctc gccgccgaag
     8281 ccgtagtcgc cgctcagcag gccgtcgacg aaatacttga agccgacagg cacctcggcc
     8341 agccgccgcc ccagcgaggc ggccacgcgg tcgatcatcg agctggagac gagcgtcttg
     8401 cccacagccg cgtcgccgcg ccatgccgga cggttctgga acagataccc gatggcgacc
     8461 gccagatagt gattcgggtt catcagtccg gcgctgcggg tgacgatgcc atggcggtcg
     8521 aagtcggggt cgttgccgaa ggcgatgtcg aagtcgtcct tgaggcgaat caggccggtc
     8581 atggcgtagg gggacgagca gtccatgcgg atcttgccgt cgtggtcgac ggtcatgaac
     8641 cggaaggtcg gatcgacata ggggttcacg atgtcgagcg tcaggccgta gcggtcggcg
     8701 atgggttgcc agaaggccac acccgcaccg cccatcgggt caacgccgat tttcagcccc
     8761 gcgccggcga tggcgtccat gtcgatgacc gcgccgagat cgtcgacgta gggcgcgatg
     8821 tagtcatact ccctggttgt ggaagcagcc atcgcctggg ccagcgtcaa ccgcttgacg
     8881 ccgcgcaaac cggcggccag cagctcgttg gcgcgatcct ggatccattt cgtcgtgccg
     8941 gtgtcggccg ggccgccttc gggcgggtta tatttgaagc cgccatcttc gggcgggttg
     9001 tgcgagggcg tgatgaccac gccgtcggcc cgcgcgggcg cggagtcgcg gttccaggtg
     9061 aggatagcgt gggagacgac cggcgtgggc gtgtagccgc ggccgcgctg gacgatcaga
     9121 ttcaccccgt tggcggcgaa cacttcgatg gccgtcgata gcgccggttc cgacagggcg
     9181 tgggtgtcca taccggcgaa gagcggaccg gtgatgccct gtccggcgcg gtattcgctg
     9241 atggcctggc agatggcgag gatgtgatcc tcgttgaagc tgccgtcggt cgacgtgccg
     9301 cgatggcccg acgtgccgaa agtgacgcgc tgcgccggat cggccacgtc gggatgattg
     9361 gaataataag ccgcgacgag gcgggggatg ttgaccagca tctctggtgg cgcgggtttg
     9421 ccggcgagtg ggtggagtgt catggagctt gattatcctt cctgaaattg attatctgct
     9481 tcagtcgatt aaagtatcca ccagtgtttg aactccccag agttccacac cgtaatcagc
     9541 cattgcccgc cgaagttcat catcggcgcg taaatcgtca tccccggtta tgattgcatc
     9601 ggcttgacca tcgatagccg ttgccagtac ggggtggtct tttgggtctc ggcaacgcgg
     9661 tggaatcgtc gtcagcgtaa caaactcgcc ccgcaactcc agttgttcca acaaatcctg
     9721 gatatcttct gctccgatcc ctcgcctaag gcgtggacgc tgggtgacga tcttcaactc
     9781 tgtgatgagc gcttcgctga cgataaggga aatttgttct cgtatagggc ctgtaagacg
     9841 ggtagtgaaa caggtccgcc aagaagcaca cgaacccaaa tgttggtgtc aacgacaatc
     9901 cgcaacataa agacggctaa tggccttgct tgcgaacagc gctaatctcg gcgttgattt
     9961 cggccacagt cacttcgact ggcggctgtt tgtcggctaa ccgtttgatt aagtcggcta
    10021 atcgccgatc cagatcgtcc tgaattaacg cccgggcgac ttgtgcccgc gcttcaggtt
    10081 ctaattgtcg gactgcccga atcaaatcgt cgagcgataa atgaacattc gggatggtta
    10141 cggtcgtagc agtcatattg tcttccaatc cgtgtcgaca gagccttaag cgatcccggt
    10201 ctccaaaata gtatagcata tgggagaggg atcgtcagat ggataagccg gatacgccgg
    10261 attcagggac atgcccagct gtgggaagtg ttccgtaaat aaaaaccggg attctgacag
    10321 aaacccggtt tttattttct catacggcca ctcgttagcg ccgcaatatc ataaacaaca
    10381 acatccccag caacaaaacg ccaatcgcaa tggcggcgat gcccaggccg accagccagc
    10441 ccggcgtgcc ggaaccgctc gatggggtgt tgcccggcgc gggggcggtc gccgtcaggg
    10501 tctcggccac ggccgttccc gccgcatctt ccggcgctcc ggtggtgggc gtcagccgca
    10561 accggctgcc gaactccagc cccacggtct gaccctggcc cacagtgacc gaggcgttgg
    10621 cctgggtggt catctccagc gacggcgatg gcgtttggcc gacctggtag gttcctgccg
    10681 ccagattgct gaaacagatg ggatcgccca tcccggtgga gacggcctgg ccgatgatgg
    10741 tcgtgccctg ggccacggtg aaggtcacgc cggccacgta gccctcatcg gggtcgtgaa
    10801 ggccgttgcc gttggtgtcc ataaaggcgt tgacgcagat ggaagccgta tcgaccgggg
    10861 ttggcgtctc ggggggtgga gcctgggtca tggtggcgat cacggtggcc gcggccgctt
    10921 cggtcggcac gccttcggtg ggagggggcg gctgggtggc caccaccggc gcttgcgtgg
    10981 gcaggggggc gggcgtcgcc gtcgccaccg ataccgcgac aagctcgccg gtgtcgaacc
    11041 aggtatcgag gtgggcgcga cattgattca cgccggtgac gccccagtcg gccgaggtga
    11101 ataccgtgat ctggttgccg gtcgccgtca cctcgacgga gaactgctgc cacgtatcgt
    11161 ggggcgagcc ggccgcgccc cagacgacgt cggcgtcatt ccacaagccg ctgccgttgg
    11221 ggtcgatgcc gacgcgcatg ttcatttgca ggctgctatc cgatggctcc ggcacctgct
    11281 tgttcgaggc gcggccgcgg ccgtaagctg tgaagcgata gacggtgccg ggggtggccg
    11341 ggacggtctg atagacgccg ccggaccagg tgtcccagga gttgcccacc gcttgcgcgc
    11401 cggagccgct gtggacgaag gccgggttgg tttccacgct ccattttggc tggtagtggt
    11461 aggcgaccat gcactcctcg cctgagcgcg gcgtctcgcg atgccagcgc ccccagcccg
    11521 tggccgcgcc cttggcgtcc gggggcgggt cgaagccggg attattcagc aggttgccgg
    11581 actgggcaat gggggcggca tagctccgac cggccaacag aaaggtcaga ccaagcaaca
    11641 acgccggaat gagtagcaga agtttatttt tcatgtgttt ttattgtcgt ggagcagcct
    11701 cgtctatcgg gctattccag ctcatcgata ttttgaaatc gccgctcgcc accttcatcc
    11761 ggtggtttcg tcgcgggcag attgcgagat tgtctgatca gcaagattaa cacagcgccg
    11821 aacaagagca aaccgccaaa gaaaaggacg cccacgccaa aaagccgcag caacagactg
    11881 ccgccattat tggccggttg gacggcaacg ctcgtgggcg gcgcggccgt cgtcatggtg
    11941 ggttggagcg cggcgcttgt ggcctgggcc acggcttctt cgatcacgct gccgaaggcc
    12001 tgatacagtt cgccgccggc cgtcaggctc aaggcccagt cgccgccggt cgtcaggagc
    12061 tcgcccgggg caatggagcg cgtgacgcga taattgcccg gcaccagccc gcccaggcac
    12121 cttggctcgg agcggccgtc ggtgatgtaa ttggcgacga cggcctcggt gttgtagacc
    12181 gtgaaggcca cgcccgcgcg caaggcctcg cccggatcgt ggatgccgtt gcgattcagg
    12241 tcgtcgaaag cggtcaggca gatggccgcc tcgcggggtt tcccggtggg ctgcggggtg
    12301 ggcgggggga atgtggccgt cggcggggcg atgatctccg gcgcgatggt cgcttccggc
    12361 gtcgaatagc cgacgatcag ggcgtcgccg gggttgatga tcgattgttc ggtcaggccg
    12421 ttgagcgcca gcagatcggg caggctgagg cccgcccgcg cggcaatgac ccacagcgac
    12481 tcgccgggct ggacgatgac gacgattgcg ccggtggcat cgggcgtggg cgacacccag
    12541 ggcggcgggg caaacgggag cgttggctcg gccgtcgggc cggaatcggg gatcggtgtt
    12601 tccgcggccg gcgtggcggt cgcgtcctgt cgcggtggaa cggccagccc gcccaataat
    12661 attcccccgg caacaatcaa cccgatcggc caactgatcg ttgacattcc agcagactcc
    12721 tggcccgctt acggcgccgg cgagacgatg acggcatcgc cggcagggtt gccgcgggca
    12781 tcgagcaccg gcagtcgttc gccggtttcg gcccagtata acccaatttc gatggaatcg
    12841 gtcgccgggt taaagggacg attaaggtgc aactcatgat gttcgcccac gacgatgccc
    12901 gcctgccaac ggtcgggggg ccacaagcca cgccctggcg ggccgtcgta ttgggccagc
    12961 atcccttcgg gaccaaggat atggatgaac tgggtgggca ccggcccggc ggccgcgctc
    13021 tcccaatcca tggcgaccat gagcgtatcg ggagccgtcg cttcggtcgt ggcctgatgc
    13081 aacaggaagg tttcgccaaa gatcgccacc ggctcatttg tcgccgggcc gggcataatg
    13141 gcgtagcgcg tgtagagcga gtaaggctca ggctccagat cgccgcgcgc gagggggccg
    13201 ggcgcgactt ccacccgcgc gggtggatgg acggcggcca ggaattcgag cgaatcgtag
    13261 ggccaggcat aaacgacgga tgccgggcca acgagcggag gcagcccctc gtccggcgag
    13321 aagggcgtgt agcggtggcc atccagcagg aagcgcacgg aaggccagcc atcagggaag
    13381 cgacggtcaa gatagacggc cgcgccggaa gtcgcgtcgg ctgccgcgga ataggccagc
    13441 tcggccgccg ccgattcgaa cagatagccg acgtcgggct gttgagcata gtcggcgtaa
    13501 tcgcgcattg tcagcgcggc gctcccggcc agcagcagaa cgaccgccgt tcgccgcaac
    13561 gccacgggca ggcgcggcca gctccataat agccccagcc cgatggccgg gaagaagagc
    13621 gcgcccggca gcacgcccac ggcgcgcaga aagtggggcg catcttcggc caggatggtg
    13681 gggccgagca tgaccagctg ccagatgagc agcagggccg ccggcggccg ccgccaatgg
    13741 cgcaggcacc acaccagccc gacgaggaag ggcacgacca tgagccaatc gaacaccggc
    13801 cggccggcgg gcacgggcgt cccataactc agccaggcgt tgtggcgcaa gatgtcgtcg
    13861 ccgcgccaga tgtacatgcc cacggcgcgg gccgtggaac ccagcagagc gtccagcgga
    13921 ttaccgccgt tgatcgtcgg gctgaggatc gacacctgcc cggcgcggcc gaaaatgagc
    13981 gacggctccg cgagcatgat aatgcccagc ggagcgacga caagccccgc gccgagggcg
    14041 aaccaaagga ttcggccgcc gctccagagg cgctcgcggc ggccggtgag gacgaggtaa
    14101 acagcgaaca gggccaggat cagcggcgtg aagcgcacgg cgagataggt gtagaagctg
    14161 aggccataga ccgccccggc cgccagccac agccctgtcg ctcgcctgtc gttctgcgtc
    14221 tcgcgccagg cgcgcgtgcc cagccagaag gtcagcgcca gcaggggcgg cagcagcccg
    14281 gcccgcaagc cgatgcgccc cagatggaca ggccagaagg tgatggccca caaggtggcg
    14341 gcgaacagcc ccgtggcgcg gccgaaccag tcgcgggccg gcagataggc cggcagggtc
    14401 gccagcgccc cgatgacggc cgccggcaag cgcagggcga agaccgtggg gccgaggagc
    14461 gcgaaggaga tggcgacgag atagatatag gacggctcgc ggccgttgtt ggccgggaag
    14521 aagagcgcgt gcccgccgcg caagacgccc agcgcgtcga ggccgttata ggcttcgtcg
    14581 cgataggggc cggggggcca cacgcccagt tgatagagcc gcagcccggc ggccagcacg
    14641 atgaccccaa gcaataaagc ccatccccgc aaggatgggc cgcgtactga ccggattgcc
    14701 cggcgaatgc ttggccggta ctgtgctgat tgaggcatgg aaccgccggc gctcagggcg
    14761 cctgggggtg attggattca gctaaccggg gaaagatagc acattttagg gcctggttca
    14821 aaaccggtct gaatgcgccc acggtttttc agtagccggc tgcgctgtaa cgccggattc
    14881 ttatccggcg ccgcggcaag ggcaatcacc cggttaagaa accggggtta catgcatgtg
    14941 tcatgccata taaagccgct ttgcacaatt ggaacggact actgaaaaac cccagggcaa
    15001 tcgcatgaag aatattggac cgcgaatgaa cgcgaatgaa cgcgaat
//
LOCUS       DAOMQG010000041        15027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1717_length_15027_cov_10.605063, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000041 DAOMQG010000000
VERSION     DAOMQG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1717_length_15027_cov_10.605063"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLC06_04085"
     CDS             <1..829
                     /locus_tag="PLC06_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRO89656.1"
                     /translation="QAGSQIILTNSFGGTRFRLKLHNLQDRVHELNRAAARIARLEAD
                     NAPHLVLVAGSIGPTGELLVPLGSMEFSSAREAFAEQARGLVAGGVDLLWIETMSDLE
                     EVRAAVEGIRSVTDLPICATMSFDTNGRTMMGITPERAVRTMSGWGLDAIGANCGNGT
                     DELENVIYAMHQEAPEVTLIAKANAGLPQWRDNVLHYDATPSSMAEYAQRVRALGASL
                     IGGCCGNGPEHIRAIADTANVPLPIAERETLLQEKAQQREAMTIEPARERSRRRSQQ"
     gene            complement(833..2077)
                     /gene="coaBC"
                     /locus_tag="PLC06_04090"
     CDS             complement(833..2077)
                     /gene="coaBC"
                     /locus_tag="PLC06_04090"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRO89657.1"
                     /translation="MQPIRVLKGKRILLGITGSIACYKAVDLASKLTQAGAEVDVILT
                     ESAVRFVSPLSFRSVTGRPAYTDLWDLSDHVRHVGLGETADLMLIAPATANTIAKLAA
                     GMADNLLTVTALAARCPLILAPAMDGGMWDHPATQANVQVLQNRGVMLAGPAAGRMAS
                     GLEGMGRMVEPQELLGHARLALSRGGPMAGRSVVVTAGPTREPIDPVRFLTNRSTGKQ
                     GIAVAQAALDAGASVTLIAGPICEEIPFGADVVRVETVEEMAQAVLTNVEAADALFMV
                     AAVADFRPVTVAERKIKKSDNSEEGMELSLMKTTDILMAVKEQRQSSRFPRVVLGFAA
                     ETHNAADYGHEKLLRKGLDFIAINDVTVEGAGFAVDTNRVLLLGPNGVIEEIPLRSKS
                     AVAERLIHHVAEVLNADKGHTA"
     gene            2167..4374
                     /locus_tag="PLC06_04095"
     CDS             2167..4374
                     /locus_tag="PLC06_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRO89658.1"
                     /translation="MTPQTKNKRVLKLLIINAIVIALVVAWNRSQQQGSISLAIAKGK
                     QLIDMPASVARYPASYLLDTASIKQVALANPTFQAALAGKDARFHSAIPLTAGEARYW
                     QDAGCTDMNCALVSFYDFTDEGTIEGIINLDTNEVVGQWVNTAARPGGSSEVLPKVVE
                     IAAADPQVRAILGENIGAIDPLMIPMSGWLGDNDCRIDWCVDLTFEDPSGTGRIYHVF
                     VNLDKEVVARTFYTRARADRSAAKPLSQRNAFTDGCHDQYGWSVCWEMTANDGINFRD
                     ATYNGRLVFSSAKIGQIEAWYPSWPGGYRDEIGFAATVPPFGDTEVTDLSDGFEIRQL
                     FTEFTRWPNCICCYRYEETIRFYSDGRFDIVFVSHGPGCDDVSVYRPFWRIDLDLNGP
                     ENDEVWLFEDAQWKEMGEEFETFPIVKDLSPEGHKLATFDGDLHYRWSMERTDPLGLD
                     EGYLFLLQWGETEGEGPITTGPGDTFIPPRQWIDGDPLSGENIVMWYVPLLNSKKGEP
                     WWCVPDPDPDFSPCNAVLHAEPAGEIRQPTAEEVAQLQSQIGATPTPGAPTAVPQPAA
                     TPRPIDGTTAEEIMLNAGCGACHKIGSFGEAHKVGPDLTYIGLTAPDRVPGMSGEEYI
                     RQSILEPNAYLAPECPNTSCLPNIMPQNFPLRLTEEQLETLVEYLMSRQGPDPTPAPV
                     GSGLAITPAPKAIGAGKVVAPTPASNLPSTAIGVLIILFVAAISAFLIFRDRS"
     gene            complement(4444..4914)
                     /gene="smpB"
                     /locus_tag="PLC06_04100"
     CDS             complement(4444..4914)
                     /gene="smpB"
                     /locus_tag="PLC06_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035202732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRO89659.1"
                     /translation="MDQKSEKIKIVYNNKRATFDYEILERFEAGLVLTGTEIKSVRDN
                     KVSLQQSFVQARDDELWLLEANIAQYVHGNRENHEPKRPRKLLLHRREINRILTNLTQ
                     KGLTVVPTRLYLKGGRAKVEVALARGKRKFDKREDLARRDADRQVERALREKYR"
     gene            complement(4933..6393)
                     /locus_tag="PLC06_04105"
     CDS             complement(4933..6393)
                     /locus_tag="PLC06_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRO89660.1"
                     /translation="MSYDQVPSPKSSGCGTSGIVLAVLAILFGLGMGAAGFLAGRISA
                     PPSPTAASVAAVVIPTATIPPAPTTEIATVVPTPVASPISPTELPTDVPTVTPEPSAT
                     EVPPTAEVVVSTAEPEELSPPAGSPLAGVDFTTFYEVWDLIAAQYDGALPETDAMRDA
                     IISGSLETLGDNYTRYVSPDVAERMREDMQGAVEGIGAFVFENDEGLFEIVRPIDGQP
                     ADLAGVKAGDILIEIDGESVLDLSFDEVILMVRGPQGTSVNLKFLREGEKEPVEFTIV
                     RTRFEVPIVESELLPSEMTGGEAIGYIRLTEFNSNAEEKLLEALNELMAQGITGLVFD
                     LRDNPGGYLDQSIAVADAFLPDGVALFERNIRGLDQTFRTIDGDLAEAIPMVVLVNSG
                     SASASEIVAGAIQDRGRAVLVGETTFGKGSVQQIHPLSDGSELRVTIARWYTPNNNSI
                     SGEGITPDIEVETPEDLGGPEDGQLLRAIEYLLTGQ"
     gene            complement(6421..>6666)
                     /locus_tag="PLC06_04110"
     CDS             complement(6421..>6666)
                     /locus_tag="PLC06_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRO89661.1"
                     /translation="GRATLIGSKTFGKGSVQLVFDLSDGSSVHVTSARWLTPNRTQID
                     QSGLEPDVPVAITQEDIDNGRDPALAEAVSYLQANFK"
     assembly_gap    6667..6766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6767..7284)
                     /locus_tag="PLC06_04115"
     CDS             complement(<6767..7284)
                     /locus_tag="PLC06_04115"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRO89662.1"
                     /translation="MSNRFRNGLTVFMLVILAAGAFGAGYLTRELVNGRGNSSLVHAN
                     EDMELFNEAWGLIQGNFLGSIPDSRAVTYGAIRGSLATLNDPYTVFIEPAAREVERER
                     LQGTFGGIGAYISRSEETGEVLLEPIPGNPAEAAGIVAGDVLLAIDGFAIPPEMTVPE
                     IIDLIKGEKGTAV"
     gene            7521..10484
                     /locus_tag="PLC06_04120"
     CDS             7521..10484
                     /locus_tag="PLC06_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRO89663.1"
                     /translation="MTRSDVARTVVAVLIFLCTVLLSSTTAHSQDIDLNGQAEALFQS
                     LSVDERIGQLFLVTFEGDRAPIDSAIADLILNYHIGGVALLSGNNNLTGYGDAAAVPQ
                     QARELTANLQRLSLLGTSDEQPLSDEPPPSPEPDAVETNPIPLIIAAQNDGDALPMLN
                     VVAGFTALPNNMALGATWNPENARRVGEVLGREFEAAGINMLLGPSLDVLEAPSPLNT
                     GDLGTHSFGGDPYWVGQMGRAYIEGVHAGSNSRLAVAARSFPGKGSSDRPVDEEVPTV
                     RRSLDQLKQIELAPFFAVSRDPADSAATADAFIATHIRYQGLQGNIRATTAPISLDPQ
                     ALTSLLALPELAPWRQGGGVMVSDALGVRSVQLFYDDTEREFPHRQVAKDALLAGNDL
                     LYVASFARGVSNQAAELANVKDTILWFRERYETDPTFRQRVDEAVLRILKLKLRLYGG
                     DISSANVIRESFDETSDTPPSIISSSFFDIAASSLSLLAPSKDELTSRVVSPPSNNDT
                     IVIFTDIHNAQVCTTCEPVPILSETALQERILALYGPLGSGQVRPDNVISFSFADLNE
                     FLAAGTTPIPFPTAPPTPTADDGTATESPDLTPEPTSTLPADYRVQEALRDADWLVFA
                     LLNAGNRAGTDNSNALSRFLSLRPDLASTNKLVVMAFDAPYFLDSTEISKLTAYYGVY
                     SKTGAFVDAAVRALFLESPLNGAPPVSVAGIQYDLFTQTQPSSNQIIELFLIIGNEIE
                     SPSRQEPVASAIGDTLHLQTGVVIDKNGNSVPDGTLVRFILRDRVRGTVTILGDRPTT
                     GGVAQLDYVLDASMGPGQFRITAESGEATLSQEVDIIIEEDARLSIIIPTAAPTATVT
                     PSPTPTATPKPTLTPEPQPPTATPPVSEAESESGRLIGLFELRSLLGIFTGLLTITFV
                     AIVANRRLPASSLTQRLGRLLWGITGGLLVYNYYALEMPGSALFSVMGSLAGLILILL
                     GGLIGIMLYRPAQRG"
     gene            complement(10491..11399)
                     /locus_tag="PLC06_04125"
     CDS             complement(10491..11399)
                     /locus_tag="PLC06_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRO89664.1"
                     /translation="MESRGILTRYAWLSVATAIGVIALKITAYLLTGSMGFLSDALES
                     GANIIAAIITLVALLVAARPPDKEHAYGHSKAEYLSSGAEGILILLAALFIAWQALIR
                     LLDPEPLAQVGLGILVSAVAATANFVVARILMKAGREHRSDALTADAHHLLTDVWTSI
                     GVIVGVALVSLTGWHWLDPVVALIVAIQILFTGYKLVRNSANGLMDAGLPEEEVSRVK
                     EILARFDEAGVKYHALRTRRSGAQRFVSVHIQVPGDWSVQRGHELLEEIEREIRLELS
                     PVNIITHIEPVEDPVSWKDAALNRPD"
     gene            complement(11529..12545)
                     /locus_tag="PLC06_04130"
     CDS             complement(11529..12545)
                     /locus_tag="PLC06_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO89665.1"
                     /translation="MMRKKAILITGASGEIGQALVTNLAQTSNLPILTLDLHPVPKEL
                     LDYCSYIAGDILDTKLLARLVTEYDFEIIFHLAALLSTRSEFSPELAHQVNVDGTLAL
                     LQVAAEQSQRRGKPVTFIFPSSIAAYGMPNLTVKARDSKVKEWQWNNPTTMYGCNKLY
                     CEHLGIYYSKHYQQLSASRPVMLDFRSLRFPGLISAFTVPSGGTTDYGPEMLHAAAQG
                     KPYACFVREDSILPFMAMPDAVTALLELAAAPPESVNHPVYNVTSFSLSAADFRDQVM
                     HAFPDAEITFEPDLLRQGIVDSWPAELNDDAARNDWGWSPAYDLQRAFHEYLVPNIHK
                     RYHR"
     gene            12712..14049
                     /locus_tag="PLC06_04135"
     CDS             12712..14049
                     /locus_tag="PLC06_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89666.1"
                     /translation="MRQRYIFWFWMPLFASWLLMSAEGPLISAAINRLPEEVIMLAAM
                     GIVTSLSVTIESPILNLLATSTALVRDHESYLLVRRYTIQWCLALTLVAVLVAYTPLF
                     DLIVYRWLDVPDAIAEWVRPGMKIMVLWTAAIGWRRFLQGIMIRNKQTRFVAYGTVVR
                     LITSGGTVIALALWGQWPGVIIGSVGLLAGVIAEASYATITARPIIRAHLSPQSAPAP
                     DVGAPLTYRSLFGFHLPLAATSVLLLMVQPLVTSSLARLDQPTASLAAWPVVFQIMLM
                     ARAGAMALPEVIIALHHDASTFNPLRRFTLSVTAATTLIIVVFAMTPLATWYVFGVQD
                     MTQEVGDLVLVGLTWFLLYPALTVITSWLRGLLIQGRHTRSVNVGMAINLVITAGMLT
                     IGVWARWQGLPAAALALNLASVGEVLYLLRQLQRILPTGVSLLGSPKAQPIKS"
     gene            14058..14693
                     /locus_tag="PLC06_04140"
     CDS             14058..14693
                     /locus_tag="PLC06_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO89667.1"
                     /translation="MTIQIIQLPLGPLQTNCYIVACTVTGKAAVVDPGWDGRAILNQV
                     EQRGWSISHILLTHTHFDHVGGLSELKGKSAAPIYAHADAIMMLEYAPAAAEMWGFSI
                     DPPPPPDMFISDGDIIEIGELRAAVLFTPGHAPGHVCFYLADRQVLFDGDVLFQGSIG
                     RTDLPGGDYELLMRSIHDKVMTLPDETAVLSGHGPATTIGAERYSNPFLLE"
     gene            complement(14698..>15027)
                     /locus_tag="PLC06_04145"
     CDS             complement(14698..>15027)
                     /locus_tag="PLC06_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89668.1"
                     /translation="VLQWTAIKSLDPNEWYMIEMSDADERDSLPRRGFTRDPFFRVPQ
                     DWRPAINEVRRMQWKVSIIQETGRRSDGGLIYTFGGNSSRPSYFLWQGAVPTPTPSPT
                     VTPTPEP"
BASE COUNT         3153 a         4516 c         4173 g         3085 t
ORIGIN      
        1 tcaggccgga tcgcagatca tcctgacaaa ttcatttggt gggacccgct ttcggttgaa
       61 gctccataat ctgcaagacc gcgtccatga gttgaaccga gcagcggccc ggatagctcg
      121 tctcgaagcc gacaacgccc cacaccttgt gcttgtggcc gggtctatag ggccgacagg
      181 cgagcttctc gtgccgctgg gcagcatgga gttcagcagc gcgcgcgagg cattcgccga
      241 acaggcgcgt ggtctggtag cgggtggcgt tgatctatta tggatcgaaa ccatgagcga
      301 tttggaggaa gtccgcgcgg ccgttgaggg catccgttcc gtcaccgatc tgcctatctg
      361 cgcgacgatg agcttcgaca caaacggccg cacgatgatg ggcatcacac cggaaagagc
      421 agtccgcacg atgagcggat ggggtctcga cgccatcggg gccaattgcg gcaacggaac
      481 cgatgaactc gaaaatgtca tctacgccat gcatcaggaa gcgccagaag tcaccttgat
      541 cgccaaagcc aacgcggggc tgcctcaatg gcgggataac gtgttgcatt atgatgctac
      601 cccttcatcg atggccgaat atgcgcaacg ggtcagggcg ctgggagcgt cactgattgg
      661 tggatgttgc ggcaacggtc cggaacatat cagggccatt gccgacacag cgaatgttcc
      721 tctacccatt gccgaaagag agacattact gcaggaaaaa gctcagcaac gggaggcaat
      781 gacgatagaa ccggcacgtg agcgaagccg gcgacgtagc caacaatagc tctcaggctg
      841 tatgtccctt atcggcattc aacacttcgg ccacgtgatg gatcaggcgc tccgccacag
      901 ccgatttgct ccgcagaggt atctcctcga taacgccatt tgggccaagc aacaggacgc
      961 gattggtgtc cacggcgaat cccgcgccct caacagtgac gtcgttgatg gcgatgaaat
     1021 ccagcccctt gcgcaaaagc ttctcatggc catagtcggc cgcattgtga gtctcggccg
     1081 caaaccccaa aacaacgcgc gggaatcgac tggattgccg ttgttccttc accgccatga
     1141 ggatatcggt cgttttcatc aatgacagtt ccataccctc ctcggagtta tccgacttct
     1201 tgatctttcg ttcggcaacg gtgacaggcc tgaaatcggc cacggccgca accatgaaaa
     1261 gggcatcggc agcttcaacg ttagtcaaaa cggcctgtgc catctcctcg acggtctcca
     1321 cccgaacgac atcagcccca aagggtatct cttcacagat cggcccggcg atcaaggtaa
     1381 ccgaggcccc ggcatccagc gcagcctggg cgacggcgat gccttgcttg ccggtggatc
     1441 gattggtcaa gaaacggaca ggatcgattg gctcccgtgt ggggccggcc gtgacgacta
     1501 cgctgcgtcc cgccatcggg ccaccgcgtg acagagccag ccgggcatga cccaacagct
     1561 cctgcggctc gaccatgcgc cccatgccct ccaggcctga ggccatcctg cccgcggcag
     1621 gcccggccaa catcacaccg cgattctgca atacctgaac gttggcctgc gtcgcggggt
     1681 ggtcccacat tccgccatcc atcgccgggg ccaggatgag cggacagcgg gccgccagcg
     1741 ccgtgacagt cagcaggtta tcggccatac ccgcggccag cttcgcgatg gtgttggcgg
     1801 tcgcgggcgc gatcagcatc aaatcggccg tctcccccaa cccgacatgg cgaacatgat
     1861 cgctcaagtc ccacaaatcc gtatatgccg gtcgaccggt gacggaacga aacgagagcg
     1921 gcgacacaaa tcgcaccgca gactcagtca ggatcacatc aacttccgcg ccggcctggg
     1981 tcagcttcga ggctaaatct accgctttat agcaagcgat ggagccggtg atgccgagca
     2041 ggatgcgctt gcctttcaat acgcgtatgg gttgcatgcc cttagtgtag caaaagcctt
     2101 caggttcgcc atgatgggta cgcgcctaaa ctggtagttc gaccgaggag ggctataatc
     2161 tcaattgtga cacctcagac gaaaaacaaa cgcgtcctca agttgctcat catcaatgcc
     2221 atcgtgatcg ccctggtcgt tgcctggaat cgtagccagc agcagggcag tatttcgctg
     2281 gccatcgcca aaggaaaaca gctcattgat atgccggcca gcgtggcgcg ctatccggcc
     2341 tcctatttgc ttgacaccgc aagtatcaaa caggtcgctc tggcaaaccc gactttccag
     2401 gcggccttgg cgggcaagga tgcccgattc cactcggcca ttccattgac ggccggtgaa
     2461 gctcgctatt ggcaagacgc cggctgcacg gacatgaatt gtgccttggt gtccttctat
     2521 gatttcacgg atgagggcac tatcgagggt atcatcaatc ttgacacaaa tgaggttgtc
     2581 ggccagtggg tcaacacggc cgcacggccc ggcggatcga gcgaggtgct gcccaaggtt
     2641 gtggagatcg ccgcggccga tcctcaggtg cgcgccatcc tgggcgagaa tatcggcgca
     2701 atcgatccgt tgatgatccc catgtcgggc tggctgggcg acaacgattg ccgcatcgac
     2761 tggtgcgtcg atttgacttt cgaggatccg tccgggaccg gccgcatcta ccacgtcttt
     2821 gtcaatctcg ataaagaagt cgtggcgcga acattctata cacgcgcgcg cgcagatcgc
     2881 agcgccgcca aacccctgtc ccagcgcaac gccttcaccg acggttgcca cgatcaatat
     2941 gggtggagtg tttgctggga gatgacagcc aatgacggca tcaacttccg tgatgccacc
     3001 tacaacggcc gcctcgtatt ctccagcgcc aagatcgggc agatcgaagc ctggtatccc
     3061 agttggccgg gcggctatcg ggacgagatc ggcttcgccg cgacggttcc cccttttggc
     3121 gatacggaag tcacggacct gagcgacggc ttcgagattc ggcaactctt caccgagttc
     3181 acgcgctggc ccaattgcat ttgctgctat cgttacgaag agacgatacg cttttattcg
     3241 gacggccgat ttgacatcgt ctttgtctcc cacggccccg gttgtgatga tgtatcggtc
     3301 tatcggccgt tctggcgtat tgacctggac ctgaatggcc cggaaaatga tgaggtctgg
     3361 ctcttcgaag atgcccaatg gaaggagatg ggcgaagagt tcgagacatt ccccatagtg
     3421 aaggacttat ctcccgaggg tcacaagctg gccacgttcg acggtgatct gcattatcgc
     3481 tggtcgatgg aacgaacgga ccccctgggt cttgatgaag ggtatctctt cctgctgcaa
     3541 tggggcgaga cggaaggtga aggccctatc acaaccgggc caggcgatac cttcatcccg
     3601 ccgcgccaat ggatagatgg cgatccgctg tccggcgaaa atatcgttat gtggtatgtg
     3661 cccttgctca actccaagaa aggtgagcct tggtggtgtg tgcccgaccc ggacccagac
     3721 ttctcgcctt gtaatgcggt gctgcacgcg gagccagcgg gagagataag gcagccgacc
     3781 gccgaagagg tcgcccagct tcaatcgcag atcggggcaa cgcctacgcc cggcgcgccg
     3841 accgccgtcc cccagccggc cgccacgccc cgtccgattg acgggacgac ggccgaagag
     3901 attatgctaa acgcgggatg cggcgcctgc cacaagatcg gttccttcgg cgaggcgcat
     3961 aaagtggggc ccgacttgac ctatatcggc ctcactgccc cggatcgagt gccggggatg
     4021 agcggcgaag aatacattcg gcaatcaatt ctggagccaa acgcttatct ggcccctgaa
     4081 tgccccaata cgagctgtct gccaaacatc atgccccaga atttccctct gcggctgacc
     4141 gaagaacaac ttgaaactct ggtcgaatat ctgatgagcc ggcaaggccc ggaccccaca
     4201 cccgcgccgg tcggctcagg cctggccatc acgcccgcgc cgaaagccat tggggcgggc
     4261 aaggtcgtcg cgccaactcc ggcgtcgaac ttgccttcga ccgcgatcgg cgtgctcatc
     4321 atcctgttcg tggccgcgat cagcgccttt ctgatcttca gggatcgctc ctaattacga
     4381 tcaaaaaaag agggtgtggc tccaatcaag ccacaccctc tttcattcat tgccccagcc
     4441 gtcttaccgg tacttctcgc gcaatgcacg ctcaacctgt cggtcggcgt cgcgtcgggc
     4501 aagatcctcg cgcttgtcga acttgcgctt gcctcgcgcc agggcaacct ccaccttggc
     4561 tcgtccccct ttcagataca gccgcgtcgg cacaacagtc aatccctttt gggttaaatt
     4621 cgtgaggatg cggttgatct cccgccgatg gagcagcagc ttgcgcggcc gtttcggctc
     4681 gtgattctcg cgattcccat gaacgtactg ggcaatattg gcctcaagca accatagctc
     4741 atcgtcgcgc gcctgaacga aactctgctg caagctcacc ttattatccc tgacggattt
     4801 gatctcggtg ccggtcagga caagccccgc ctcaaaccgc tccagaatct cataatcaaa
     4861 agtggcgcgc ttgttgttat agactatctt gatcttctct gatttttgat ccataccaca
     4921 aacttgcgcc tcttactgac ctgtcagcag atattcgatg gcccgcagca attggccgtc
     4981 ttccggcccg cccagatctt cgggcgtctc cacttcgata tccggcgtaa tgccttcacc
     5041 gctgatcgag ttattattgg gcgtgtacca gcgagcaatg gtcacgcgca actcggaacc
     5101 atccgacagt gggtgaatct gctgcacaga ccccttgccg aatgtcgtct cgcccaccag
     5161 caccgctcgg ccgcgatcct gaatggcccc tgccacgatc tcggaggcgc tggcgcttcc
     5221 tgaattgacc agaaccacca tggggattgc ctcggcaagg tcaccatcga tcgtgcggaa
     5281 ggtctgatcc aggccacgga tgttgcgctc gaatagcgcg acgccgtcgg gcaggaatgc
     5341 gtcagcaacc gcgatggatt gatcgagata gccgcccgga ttgtcccgta aatcaaacac
     5401 caatccggta atgccctggg ccatcagttc gtttagcgcc tcgagcaatt tctcttctgc
     5461 gttgctgttg aactcggtca ggcgaatgta accgatcgcc tctccgcctg tcatctccga
     5521 aggcagcagt tcgctttcga cgataggcac ttcaaaccgt gtacggacaa tcgtgaattc
     5581 aaccggttct ttctcgccct cgcgcaaaaa cttcagatta acggatgtcc cctgtggccc
     5641 ccgaaccatc aggatgacct catcgaagct caaatcgagc acggattcgc cgtcgatctc
     5701 gatcaggata tcaccggcct taaccccggc caaatcggcc ggttgcccgt caatcggccg
     5761 cacgatctcg aacaaaccct cgtcattctc gaaaacaaac gcgccgatac cctcgacggc
     5821 cccctgcata tcttctctca ttcgctcagc tacatcgggg gagacgtaac gcgtgtaatt
     5881 atcgcccagc gtttcgagag agccggagat aatcgcatca cgcatggcat ccgtttccgg
     5941 caaagcaccg tcatactggg cggcgatcag gtcccacact tcatagaacg tggtgaaatc
     6001 aacgccggcc agaggtgatc cggccggtgg ggaaagctcc tcgggttcgg ccgtggaaac
     6061 cacaacctcg gccgtaggag gcacttcggt agcactaggc tccggagtca ctgtcggtac
     6121 atcagtgggc agttcagtcg gagagatggg gctggcaacc ggagtcggca caacggtggc
     6181 gatctcggtt gtcggagcgg gcggtatcgt ggcagttggg atcacaactg ccgccacgct
     6241 cgcggccgtg ggagatggcg gcgccgatat gcgtccggcc agaaagccgg ccgctcccat
     6301 acccaggcca aacaggatgg ccaagacggc aagcacgata cccgatgttc cacacccgct
     6361 tgattttgga gacggcactt gatcgtagga cataattatg gattacctgg acgagaaagt
     6421 ttacttgaag tttgcctgca aataggaaac ggcttccgcc aaagcaggat cgcgaccatt
     6481 gtcgatatct tcctgagtga tcgcaaccgg cacatccggc tccaggccgc tctgatcaat
     6541 ctgggtgcga ttcggcgtta gccagcgggc agaagttaca tggacggatg aaccatcact
     6601 taggtcaaaa accagttgta ccgagccttt gccaaacgtt ttgctgccga tgagagttgc
     6661 acggccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg ccgtcccttt
     6781 ttctcccttg atcaaatcaa tgatctccgg cacagtcatc tcaggcggaa tcgcaaagcc
     6841 atcaatcgcc agaaggacat cgccggcaac aatgccggcg gcttccgccg ggttgccggg
     6901 gatcggctcc agcaacactt cgccggtctc ctcggaacgc gatatatatg cgccaatacc
     6961 cccaaatgtg ccttgcagcc tttcccgttc cacttcgcgc gcggccggtt cgatgaatac
     7021 cgtgtacgga tcattcagag tagccaggga gccacgaatc gcaccgtatg tcacggcgcg
     7081 ggaatcagga atactgccca ggaaattgcc ctggatcagg ccccatgctt cattaaacag
     7141 ttccatgtcc tcattggcgt gaaccaacga ggaattcccg cggccgttaa ccagctcgcg
     7201 ggtcaggtat cctgcgccaa aagcgccggc agccaggata accagcatga aaacagtcag
     7261 accattgcgg aatcgattcg acatacacac tcaagcgccc ggagggcgcg tgaagagatt
     7321 ttaacacagg tgttcggtcg ggcaatccgc gctataatca agaccatcgc tgttagcaag
     7381 gattaatccc cgctacggtg atttttataa accgacgcgc tttgattcca agcaaaacgc
     7441 cgcttgcccc ggaaatcagc gcgacggatg tggatttgtg tgaggagtga gccctaaatt
     7501 gggatcgggc taaatttgtc atgacaagat cagatgtagc gcgcacggtt gtggcggtac
     7561 tcatattttt gtgtaccgtc ctgctgtcgt cgactaccgc ccacagccag gacatcgacc
     7621 tgaacggaca ggccgaggct ctgttccaat cgctctcggt cgatgagcga atcgggcagt
     7681 tgttcctggt gactttcgag ggtgaccgcg cgccgattga tagcgccatc gccgatctga
     7741 tccttaatta ccacatcggc ggcgttgcac tcctctccgg aaataacaat ctcaccggct
     7801 acggtgatgc ggccgccgtc ccccagcaag ctcgcgaatt gacggcaaat ttacagcgat
     7861 tgtcattgct gggcaccagc gatgagcagc cgcttagtga cgaaccccca ccatctcctg
     7921 aacccgacgc agtagaaacg aatccaattc ccctaattat cgccgcgcaa aatgacgggg
     7981 acgcgcttcc aatgctcaat gtcgtcgccg ggtttaccgc gcttccgaac aatatggctc
     8041 tgggggcgac ctggaatcca gaaaatgccc gccgcgttgg cgaagtgctc ggccgcgagt
     8101 tcgaagcggc cggcatcaat atgctgcttg ggccatctct ggacgtgctg gaggccccat
     8161 ctcctctgaa caccggagat ctgggcacac attcatttgg cggcgatccc tactgggttg
     8221 ggcaaatggg ccgggcctat atcgaaggcg tgcacgccgg cagcaacagc aggttggcgg
     8281 tggcggcgcg cagtttcccc ggaaagggga gcagcgaccg gccggtagat gaagaagtcc
     8341 ctactgtccg acgctctctt gatcagctca agcaaatcga attggcccca ttctttgccg
     8401 tatcgcgcga cccagccgat tccgcggcaa cggccgacgc gttcattgcc acccacatcc
     8461 gctaccaggg cttgcaaggc aatatccgcg cgacgactgc tccgatcagt ctcgaccccc
     8521 aggcgctgac ctctttgctc gccctgcccg aacttgctcc ctggcgacaa ggaggtggcg
     8581 tgatggtgtc ggacgccttg ggcgtgagaa gcgtgcagct attctatgat gacaccgaac
     8641 gggagttccc tcatcgacaa gtggccaaag atgctctcct ggccggaaac gatctgctct
     8701 acgtagcaag ttttgctcgt ggggtctcga atcaggcggc cgagctggcc aacgttaagg
     8761 acacaatcct gtggtttcgc gagcgatatg aaactgatcc caccttccga caacgcgtcg
     8821 atgaagcggt attacgcatt ctgaaattga agctccggct ctatggcggc gatatttcgt
     8881 ccgccaatgt cattcgagaa tcgttcgacg aaacgtcgga cacccctcca tcgatcataa
     8941 gttcttcatt cttcgatatc gccgcatcat cactctctct cctcgcgccc agcaaggatg
     9001 agctgacgag ccgcgtcgtc agtccccctt ccaacaacga cacgatcgtc atattcaccg
     9061 acatacacaa tgcccaggta tgcacgacct gtgagccggt tccgattctg tccgaaacgg
     9121 cacttcagga aaggattctc gctttatatg gacctttagg cagtggtcag gtccgaccag
     9181 ataatgtcat cagcttttca ttcgccgatc tcaatgaatt cctggccgcc ggcacaaccc
     9241 ccattccttt tccgaccgcg ccgcccacac cgacggccga cgacggcacg gcgacagaat
     9301 ctcccgatct gacgccggag ccgacctcga ccttgccggc agattatcgc gtgcaggagg
     9361 cgctgcgcga cgctgattgg ctggtatttg ccttgctcaa tgcgggaaat cgggccggca
     9421 ccgataactc caatgccctc agccgctttc tgtcactgcg gcccgatctg gcaagcacca
     9481 ataagctggt tgtcatggcc ttcgacgccc cttacttcct cgacagcacg gaaatcagca
     9541 agctaacggc ttattatgga gtctacagca agacgggggc tttcgtcgac gcggccgtgc
     9601 gcgcgctttt cctggaatct ccgctcaatg gcgccccccc ggtaagcgtg gcaggtatcc
     9661 aatacgacct tttcacgcag acccagccat cgtccaatca gattatcgag ttgtttttga
     9721 tcatcgggaa cgaaatagaa tccccatccc gccaggagcc agtggcttcg gcgatcgggg
     9781 acacgttgca tctacaaaca ggcgtcgtaa tcgataagaa cgggaactct gttcctgacg
     9841 gcacactggt tcggtttatc ttgcgcgatc gagtgagggg gacggtcacc atcctggggg
     9901 atcgcccgac gaccggcggg gtggcccaat tggattatgt tctggacgcc agcatgggac
     9961 cggggcaatt tcgcatcacg gccgaatccg gcgaagcaac cctgtctcag gaagtcgata
    10021 tcatcatcga agaagacgcg cggctatcga tcatcatccc caccgccgcg ccgacagcga
    10081 cggtgacgcc ttcgccaacg cctacagcaa cgccaaagcc taccctcacc cccgaaccac
    10141 agccgccgac agcaacgccg cccgtatccg aggcggaaag cgagtcgggt cggctcatcg
    10201 gcctctttga attacgttca ttattaggta ttttcaccgg gttgctgacg atcacctttg
    10261 tggcaattgt ggcgaaccga cgattgcccg caagctccct cactcaacgc ctcggccgct
    10321 tgctgtgggg tatcaccgga ggcctgctgg tgtacaacta ctacgccctg gaaatgcccg
    10381 gctccgcgct cttctcagtc atgggcagcc tggcggggct gatcctgatc cttctcggcg
    10441 gcctgatcgg gattatgctc tatcggccgg cgcagcgtgg gtaatctcgt ttagtcggga
    10501 cgattgagcg ccgcgtcttt ccaggagact ggatcctcaa ccggctcgat gtgcgtgatg
    10561 atgttgacgg ggcttaactc aagacgaatc tcgcgctcaa tttcttcgag cagctcatga
    10621 ccgcgctgca cgctccagtc acccggcacc tgaatatgga ccgagacaaa gcgctgcgcg
    10681 ccggatcgtc gagtgcgtag tgcatggtat ttaacccctg cctcgtcgaa cctggccaat
    10741 atctctttaa cccggctgac ctcttcctcc ggcaggcccg cgtccatcag gccattggcg
    10801 gaattacgaa cgagcttgta accggtgaat agaatctgga tggccacgat cagggccaca
    10861 accgggtcca gccaatgcca tccggtaagg ctgacgagcg ccacgccgac gatcacgccg
    10921 atagacgtcc atacgtcggt cagcaggtgg tgggcgtcgg ccgtcaacgc atccgagcga
    10981 tgttcccgcc cagccttcat caaaatccgg gcgaccacaa aattggcggt ggccgccacg
    11041 gccgagacaa ggataccaag ccccacctga gccaatggtt cagggtccag caaacgaatc
    11101 aacgcctgcc aggcaataaa gagcgcggcg agcaggatca gaatcccttc tgccccggac
    11161 gacaaatact cggccttgct atgaccatag gcatgctcct tgtcgggggg gcgcgccgca
    11221 accagcaatg cgaccagcgt gatgatggcg gcaatgatat tcgcgccgga ttccagggca
    11281 tcggagagaa agcccattga gccggtcagc aagtaagccg tgatttttag agcgatgaca
    11341 cctatggctg tggcaacaga tagccaggca tagcgggtca atatcccacg agattccata
    11401 tgctgattat cgcctgccat cccgtggcaa tcaatccgga acgatcaaac agccgttagg
    11461 ttaccctaag cgagcaaccc caccccacga aaataaacag ggtgttgcgg agcgttgcaa
    11521 tagtctcatt agcgatggta tcgcttgtga atattgggaa ccagatactc gtggaacgcg
    11581 cgttgaagat catacgcggg cgaccacccc caatcattgc gcgccgcatc atcattcaat
    11641 tcagccggcc aactatctac gattccctgt cgcagcagat caggctcaaa ggtaatttcc
    11701 gcgtcgggga aggcgtgcat aacctgatcg cgaaaatcgg ccgccgacag gctgaagctg
    11761 gtcacattat acacagggtg gttcacgctc tcgggcggag cagccgccaa ctcgaggagc
    11821 gccgtgaccg cgtccggcat ggccataaac ggcaggatgc tgtcctcgcg cacgaaacaa
    11881 gcgtaaggct ttccctgggc ggcggcatgg agcatctcgg ggccataatc cgtcgtgcca
    11941 ccggagggga cagtgaaagc gctgatcagg ccggggaaac gcaggctgcg aaagtcgagc
    12001 atgaccggcc ggctggccga gagctgctgg taatgcttgc tatagtagat acccaggtgc
    12061 tcgcaataga gcttgttaca gccgtacatc gtcgttgggt tattccattg ccactcttta
    12121 actttgctgt cgcgcgcctt aacggtgagg ttgggcatcc cataggcagc aatcgagctg
    12181 ggaaagatga aggtgaccgg cttgccgcgc cgctgggatt gctcggccgc cacctgcagc
    12241 agtgccagcg tcccatcgac attgacctgg tgggccaact ccggcgagaa ctcgctgcgc
    12301 gtcgatagca atgcggccaa gtggaagatg atttcaaaat catactctgt caccaaccgt
    12361 gccaacagct tggtatcgag gatgtcgcca gcgatgtaac tgcagtagtc cagcaactct
    12421 ttggggacgg ggtgcaggtc aagcgtcaga atcggcaggt tggaagtttg ggcaagattg
    12481 gtgacgagcg cctggcctat ctcaccggac gcgccggtga tcagaatagc ttttttccgc
    12541 atcatgctac agctcctgtg gaaatggaat gttgggagac agaggagtac tgacgaccca
    12601 catgggccat gctatactca ctgtccggga ttcatcataa tccatattgt accgaaagcg
    12661 cgagaaggta tctaagaatc acgatattgt caccagcgct ggagtgaatg catgcgtcaa
    12721 cgatatatat tctggttctg gatgccgctg tttgccagtt ggctgctcat gtcggccgaa
    12781 ggacctctca tttccgccgc catcaaccgc ctgccggaag aggtgatcat gctggcagca
    12841 atggggatcg tcaccagtct ctcggtcacg atcgaaagcc ccatcctcaa cctgctggca
    12901 acttcgacgg cgctggtacg cgatcatgag tcctacctcc tcgtgcgtcg atacaccata
    12961 caatggtgcc tggctctgac gctggtcgcc gtcctcgtcg cctacacccc gctttttgac
    13021 ctgatcgtct atcgatggct ggacgtgccc gacgcgatcg ccgaatgggt acggccaggc
    13081 atgaaaatca tggtcttgtg gacagcggca attggctggc ggcgcttcct gcaagggatc
    13141 atgatccgca acaaacagac gcgctttgtg gcctatggga cggtcgtgcg attgatcacc
    13201 tccgggggga cggtcatcgc gctggcgctg tgggggcaat ggcccggcgt cattatcgga
    13261 tcggtcgggc tattggccgg ggtgatcgcc gaagcatctt acgcgacaat caccgcgcgg
    13321 ccgatcattc gcgcccacct gtctcctcaa tctgctcccg cgccggatgt cggcgcgcct
    13381 ctcacatacc gctctctgtt cggattccat ttaccgctgg ccgccacttc agtattgctg
    13441 ctcatggttc agccgttggt gacttccagt ctggcccggc ttgaccagcc cacagcttct
    13501 ctggctgcct ggcccgtagt cttccagatc atgctcatgg ctcgtgccgg agctatggct
    13561 ctgcccgaag tgatcatcgc tctgcaccac gatgcttcca cgtttaaccc gcttcgtcgg
    13621 ttcaccttgt cggtgacggc ggcaacgact ctgatcattg tcgtcttcgc catgacgcct
    13681 ctggcgacct ggtatgtatt tggcgtgcag gatatgaccc aggaagttgg cgatctggtt
    13741 ctggtgggcc tcacatggtt tctcctttac ccggctttga ccgtcatcac gtcctggttg
    13801 cgaggtttac tgatccaggg ccgccacacc cgttccgtga atgtcggcat ggccatcaac
    13861 ctggtcatta ccgccggcat gttgacgatc ggggtatggg cacgctggca aggtttgccg
    13921 gcggccgctc tcgccctgaa cctcgcatct gtcggcgagg tgctctatct gcttcgacaa
    13981 ttgcaacgta tcttgccgac cggcgttagc ctgttaggct cgccaaaggc tcaacccatc
    14041 aaatcctgaa ttcatctatg accatacaaa tcattcaact ccctctaggc cctcttcaga
    14101 caaactgcta catcgtggcc tgcacggtaa ccgggaaagc ggccgtggtc gatcccggct
    14161 gggacggccg cgccatactg aaccaggtcg agcaacgagg ctggagcatc agccatatcc
    14221 tgctcacgca tacccatttc gatcatgtgg gcggattaag cgagttgaag ggaaaatcgg
    14281 ccgcgcccat ctatgcgcat gccgacgcga taatgatgct ggagtatgct cccgccgccg
    14341 ctgaaatgtg ggggttctcc atcgatccgc cgccaccccc ggacatgttc atctcggacg
    14401 gagatatcat cgagatcggg gagttacggg cggccgtatt gttcacgccg ggccacgcgc
    14461 ctggccacgt ttgcttctac ctggccgacc ggcaggtgct gttcgatgga gatgtgctct
    14521 ttcagggcag catcggccgc accgatttgc cgggaggaga ctacgagctg cttatgcgca
    14581 gcattcacga caaggtgatg acgttgccgg atgaaaccgc ggtcctctca ggacacggtc
    14641 cggccacgac tatcggcgct gaacgctact cgaacccgtt cttgttggaa tgatcgttta
    14701 aggctccggt gttggcgtga cggttgggct tggtgtcggt gtgggcacgg ctccctgcca
    14761 caggaaatag gacggccggc tgctattccc gccaaaggtg tagatcaggc caccatcgct
    14821 gcgccggcct gtttcctgaa taatactgac cttccattgc atccggcgga cttcattgat
    14881 cgccggtcgc caatcctgag gcacacggaa aaacggatcg cgggtaaagc cccgacgggg
    14941 gaggctgtcg cgttcgtcgg catccgacat ttcaatcata taccactcat tcgggtccag
    15001 gctcttgata gccgtccatt gaagcac
//
LOCUS       DAOMQG010000042        14651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1771_length_14651_cov_10.813785, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000042 DAOMQG010000000
VERSION     DAOMQG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14651
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1771_length_14651_cov_10.813785"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..>1192)
                     /locus_tag="PLC06_04150"
     CDS             complement(46..>1192)
                     /locus_tag="PLC06_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRO89669.1"
                     /translation="AMREMEYLQLDPLQIIARSQDIQLHSRVLDYAPGLWEEATYGRR
                     EFFDWGGWLATRPMDELPHWRVAMHRERDNSGVVDSRIHRMGIEHAEAVAEMRAILRE
                     RETVSNRDFSMATRTRTQSYRGRKDSALALYYLWRTGEVMTHHREKFERVYALTEAVA
                     PTHLIRESDESESERFLLRKDIAFAGLSRVRRTSDAFHRGVTFDQAREGLLTDGEVIE
                     VKVEGWKPMHYALASDGQALLDLSAGRIPGAWASTAATTTQEEVVFLSPLDPVSARGR
                     AKVLFGFDYVWEVYKPVEKRRFGYYTLPVLWGDRLVARFDSKLDRPSNTFVILGLWLE
                     DEALGRDEAFAAALAAGMRRFVAFLGADKLDASAIGEPLLRGSVMAL"
     assembly_gap    1193..1292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1454..2764)
                     /gene="aceA"
                     /locus_tag="PLC06_04155"
     CDS             complement(1454..2764)
                     /gene="aceA"
                     /locus_tag="PLC06_04155"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HRO89670.1"
                     /translation="MFKPYNVAKLKDDWELNKRWQGIIRPYSAEDVIRLRGSVQIEHT
                     LARMGAERLWRLMQQEDFVAALGAVTGNQAVQQVQAGLKAIYVSGWQVAGDNNSSAHT
                     YPDQSLYPADSVPNLVKRINNALQRADQIYHLQDEEDTYWYAPIVADAESGFGGSLNA
                     FELMKGMIEAGAAGVHFEDQLSSAKKCGHMGGKVLVPTREFVQKLVAARLAADVMGVP
                     SVLIARTDANGADLLTSDVDPYDQPFCTGERTVEGFYRINSGLDSAIARGLAYAPYAD
                     VVWCETATPDMAEARRFAEAIHAKFPGKLLAYNCSPSFNWKRNLGEADIARFQRELGQ
                     MGYAFQFVTLAGFHTLNAAMFELAIGYKDNGMAAYSHFQQREFQLEEEHGFRAIKHQS
                     FVGAGYFDEVQMVISQGESSTVALKGSTEEEQFEAVHQGTNGHA"
     gene            complement(2870..4456)
                     /gene="aceB"
                     /locus_tag="PLC06_04160"
     CDS             complement(2870..4456)
                     /gene="aceB"
                     /locus_tag="PLC06_04160"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HRO89671.1"
                     /translation="MAQEGIDIRGEMTPEFAEILTPEALAFVAGLQRAFNGRRLALLQ
                     RRAERQKEIDAGKMPDFLPETAEIRAAEWTVAPIPADLQDRRVEITGPVDRKMVINAL
                     NSGATVYMADFEDSHSPTWAATVEGQINLRDAIARTITFTSPEGKSYKLNERTAVLLA
                     RPRGWHLSEKHMLVDGEPISASLFDFGLYFFHNARNLIARETGPYFYLPKLESHLEAR
                     LWNDVFVMAQERLGLPNGTIRATVLIENILATFEMDEILWELRDHSAGLNCGRWDYIF
                     SAIRKFQKHPDFYLPDRAQVTMTTPFMRAYSQLVIKTCHRRNIHAMGGMAAQIPIKND
                     PEANEAALGKVRADKEREAKDGHDGTWVAHPGLVPIAREIFDKYMPTPNQIHRKRGDV
                     EVTAADLLAIPTGTITVGGLRTNIDVGIRYMAAWLSGNGCVPIYNLMEDAATAEISRS
                     QIWQWLHNPHAKLDDGRAIKVGLFRKMTDEVMAELKSQVGETEFAAGHYELAAKLFDE
                     ISVADEFTDFLTLKAYEYLN"
     gene            4755..6269
                     /locus_tag="PLC06_04165"
     CDS             4755..6269
                     /locus_tag="PLC06_04165"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO89672.1"
                     /translation="MSLDLVTIIFGMKVRQARLEAGLTLSDLSRQSEMSPSYLTEIEK
                     GRKYPRADKIVKMAAALGKEYDDLVSIKLAPSLRYLESTLSSSTIRRFPFEEFGLEPA
                     DALGLVTREPDRASALLHAVVEITRRYDLQSEEFLRAALRSYQEIHENYFPELEEAAE
                     QFIATVGARHGFDGKRLISLATLEKILGDEYGYLIDDKAIAATEELRHYRSLVVPGRK
                     PQLFINSRLYARQVRFILAREIGYRYLGIKERSFVSTPDEVDSFQQLLNDARAAYFGG
                     ALLMPREHVLSDLRQLFAMPAWDPEPFSDLLSTYDVTPEMLLYRFSELIPEFFGLKIH
                     FLRFHHATGTDNYQLVRHLNLNQLLVPSGTGLFEHHCRRWLSLRLLPEPAAGRPSSGE
                     PLPVGVQMSEFLETGDKFLCIGFGRRMVLSPGVASSVVIGFRVEPELRETIGFVDDPE
                     IPHTIINETCERCPLGPQQCDVRAAPPHILEAERERMARKMALNQLKNRSAAAE"
     gene            complement(6708..8090)
                     /locus_tag="PLC06_04170"
     CDS             complement(6708..8090)
                     /locus_tag="PLC06_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO89673.1"
                     /translation="MSTPFEPRLRTKLFIIGLILLMLGGLVLVDSRRSQAQSGPPPIS
                     QSATPSGARVSAESLNLRTGPHVSYTAVAYLMEGEEIRLVGRNRTGTWVKIAMYNGYL
                     GWVNASYIRTTTDIAALPIADVDLWGITAFVTNDPIPVYTGADAGFGLVGWAHPGEVL
                     ALNGRNDDATWVHVRLPDGRPGWARADSSFLPSGTLNDLPILTPFLDAPPPYLAPYFV
                     VYDGPGFLYEPVDSVTEGQSLGIIGRTADNHWVQVRLADGREGWIAAEIIHVSASLAN
                     VPVVHGIAAPQVVGWQGSKSAGAGDSQAQVPTATTPSKIQSIATAVPTAQATATTAPP
                     TVTPPPSTATATAGATATNAATGTPAATGTAAATTMVSPTGTAAPTQEASTDATPPGL
                     PVVYVYATPDEGTAPILRVVPGQSVVLIGRTEDAAWVKIWLPGGQEGWIRAEALQLEM
                     DVSVLPVITP"
     gene            8307..9350
                     /gene="meaB"
                     /locus_tag="PLC06_04175"
     CDS             8307..9350
                     /gene="meaB"
                     /locus_tag="PLC06_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRO89674.1"
                     /translation="MLSEKITTDWIDGARQGNPRAIARLITRVESDAAAAQTIIRAIY
                     AHTGQAHIVGITGSPGSGKSSLVNELAKSFRRGGRRVGIVAVDPTSPFTGGAILGDRV
                     RMRDLSGDDGIFVRSMASRGSLGGLARATQAAVKILDAAGYEVILVETVGAGQAEVDI
                     ARAAHTTVVLEAPGMGDDVQSIKAGILEIADILVVNKADQLGANRTVKSLEMMLHLAL
                     PALSDGHHHNDGHHRGDPHLAEPAGRATPGWDIPVLRTTATTGAGVDELAGMVDRHRL
                     YLKESGGWAVREHARGLAEIERLLRDRFIDEMVARVPLAEREALVRAVAERRLDPYTA
                     ADRLFQATGLFAS"
     gene            9378..10319
                     /locus_tag="PLC06_04180"
     CDS             9378..10319
                     /locus_tag="PLC06_04180"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRO89675.1"
                     /translation="MFGNIKLYAGSACPELAQRIADYMGIRLNRWDLITFPNENIFIR
                     LNSSVRGQDVYLIQTHDRPVHDNIMEMLIAIDCFKRDSAGRVTVVIPYLSYSQSDKKD
                     QPRVPITARLLADMIEVAGADRWVTIDLHAGQIQGFYKIPGDSLTALHIICKYIREKS
                     LDATVVTTDLGYAKGGRNYAEALGFPLAFVEKRRVGNETGREALTLIGSVEGKDVLIV
                     DDLVDTAGTVEQAVQVVRERGARDVYLAFTHAVLSPPAVERLERMNIKEIVTTDTLPI
                     PPEKRLPNMTVLTVAELLGEVILRSHEGRSVGELFNE"
     gene            10417..14367
                     /gene="secA"
                     /locus_tag="PLC06_04185"
     CDS             10417..14367
                     /gene="secA"
                     /locus_tag="PLC06_04185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRO89676.1"
                     /translation="MFKKLVNSIVGDPNKKIINNLRPTVSAASEFESRLKQLPDEAFR
                     DRTAELKERVAAGADLDDVLPEAYALVREASRRAIGLRHYDVQIMGGILLTQGEVVEM
                     RTGEGKTLVATLPLYLNALAGEGAHLVTVNEYLARRDGGWMGKIFHLLGMSVGVIGPL
                     GFSALFDPEYVNPGAELEDERLVHWRPSTRRQAYEADITYGISSEFGFDYLRDNMAVR
                     QDQLVQRNLHFAIIDEVDNILIDEARTPLIISGPAARSGKDYVRFAEYVRRLRANTAD
                     EEEEPNGHYDIDEKSRSISLTEMGIAEVEKRIEEIDSEAGDNLYDPRFYHLTYYLDNA
                     LRAQYLFKRDVDYVVQGDEVVIVDDFTGRLMPGRRYSDGLHEAIEAKEGVNVKRETVT
                     VATITLQNYFRLYDKLAGMTGTALTDAEEFDKIYELGVTPLPTNVEYIIDTGQMGLGT
                     RREKVDGAEAIVYVRPETGEPVYFKRIDFADQVYGTLDAKDQAILSEIKTVHTSGRPV
                     LVGTTSVEHSEMISNYLRRESIAHNVLNAKIHQSEALIVAQAGRKGAVTISTNMAGRG
                     TDILLGGNAEGLAAEALENEMFDRPMLIQLAFKLLDEGEEAARDTARKSNKLTEDLVD
                     ALVETEHRFAEAMVEIDQVQIVGHLARVLQEPYGIDYNDLLEVLRRVRAGELAEARIF
                     VESLDKDVALVDDIMRRWNELVRYQNIQTDEKATAQFLAEQTFEHHYNARAALIRAVL
                     GDRLDEANKLVTTIPGLAPRHIARIQELREQAKRDKEEIWRLGGLHVVGSERHESRRI
                     DNQLRGRAARQGDPGSSRFFLSLEDDLMRRFGGERLKGFMSRTNIPEDMPIESGLLDR
                     IIESSQERIEGYNFDMRKNIVEYDDVMNKQRQAIYNERREILMGEDFDLDEKVDAAFD
                     QAIEELIDHYVNDYPGFIRGEVERVVQDFTTDATNTVNVAAVLRRVGSLLPLMAELDR
                     DDLERMSPDKLTSTLVSLAHEQGRQGTNLLQLLQAMSRFLPLVPPVPNLGALAAQRGG
                     QVQTRERIRVEYLAHVETLFNEFLADYADKADRDAIWGEAQTEIDRAFSHFNLDGLSI
                     KNKDERQAHFREGVDGVLRELLLQSLSALDADQLAEALEGYIDKQQQKWRKYIGEESY
                     RNYEQLLLLQAIDREWRDYLTAMDDLRREIGLQAFGQRDPKIEYKKRSFEMFGDMRKN
                     IDRDIADRFFREISGHQAFIQQQQQEVAYKVQSQDAGYQTVKREKGKGTELRKDTPDV
                     GRNDLCPCGSGKKYKNCHGRPGMATAAAAGGNGQSAQPSKSGKKPQQQKKKARR"
     gene            complement(14420..>14651)
                     /locus_tag="PLC06_04190"
     CDS             complement(14420..>14651)
                     /locus_tag="PLC06_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRO89677.1"
                     /translation="ETAALHAAIQKVLAMGIEREGASIGTYVKADGEMGDMQNAVAVF
                     RRTGQSCYTCGTPIERIVLGGRSTHFCPGCQR"
BASE COUNT         2812 a         4602 c         4456 g         2681 t
ORIGIN      
        1 cagaaccacc actccaaaat aaacaatggg gcgcgattaa tttaattata aggccatcac
       61 actcccccgc aacaacggct ccccaatcgc cgacgcatcc agcttgtccg cgccgaggaa
      121 ggccacaaac cgccgcatcc ccgccgccag cgccgcggca aaggcctcat cccgacccaa
      181 cgcctcatcc tccagccaca ggcccaggat gacgaaggtg ttgctcggcc gatcgagctt
      241 gctgtcaaac cgggcgacga ggcggtcacc ccacaggacg ggcagcgtgt aatagccgaa
      301 gcggcgcttc tccaccggct tgtagacctc ccacacgtag tcgaagccga acagcacctt
      361 ggctcggccg cgggcgctga ccggatcgag cggcgacagg aagacgacct cctcctgcgt
      421 ggtcgtcgcc gccgtggacg cccacgctcc cggtatacgc ccggcgctca ggtcgagcag
      481 tgcttgacca tcactcgcca gggcataatg catcggcttc cagccttcca ccttgacctc
      541 gatcacctca ccgtcggtca gcaacccctc gcgggcctgg tcaaaggtca cgccgcgatg
      601 gaaggcgtcg ctcgtccggc gcacgcgcga cagaccggca aaggcgatgt ccttcctgag
      661 caggaagcgc tcggactcgg actcgtcgct ctcgcggatg agatgggtgg gggccaccgc
      721 ttcggtcagg gcatagacgc gctcgaattt ctcgcggtgg tgggtcatca cctcgccggt
      781 gcgccacaag tagtagaggg ccagggcgct gtccttgcgg ccgcgatagc tctgtgttcg
      841 cgtccgcgtc gccatggaaa aatcccggtt gctgaccgtc tcccgctcgc gcaggatggc
      901 tcgcatctcg gccacggcct cggcgtgttc gatccccatc cggtggatgc gcgaatcgac
      961 gacgccgctg ttgtcgcgct cgcggtgcat ggccacccgc caatggggca gctcgtccat
     1021 cggccgcgtc gccagccagc cgccccagtc gaagaactcc cgccgcccat aggtcgcctc
     1081 ttcccacagg cccggcgcat aatcgagcac gcggctgtgc agctggatgt cctgactgcg
     1141 ggcgatgatc tgcaaggggt cgagttgcag gtattccatc tcgcgcatgg ccnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctctgtc ttcgttaaat aacttgtccg
     1321 cgccttggca gtgggggcaa cagttcatga ttgccctgat tatacacgaa aagggggcga
     1381 ccgcccggcc gcccccttcc cacaaaagga gaaatgaaat ggagtagtgg ctgtccacta
     1441 cgagcaaact tgtttaggcg tgaccgttcg tcccctgatg aaccgcctcg aactgctcct
     1501 cttcggtcga acccttgagg gccacggtcg acgattcacc ctgcgagatc accatctgca
     1561 cctcatcgaa atagcccgcg ccgacgaagc tctgatgctt gatggcgcgg aagccatgct
     1621 cctcttccaa ctggaattcg cgctgctgga agtgggagta ggcggccatg ccattgtcct
     1681 tgtagccgat cgccagctcg aacatcgcgg cgttgagcgt gtggaacccg gccagcgtca
     1741 cgaactggaa ggcgtagccc atctggccca actcgcgctg gaacctggcg atgtcggcct
     1801 cgcccaggtt gcgcttccag ttgaacgacg gcgagcagtt gtaggccagc agcttgccgg
     1861 ggaatttggc gtggatggcc tcggcgaagc ggcgcgcttc ggccatgtcg ggcgtagccg
     1921 tctcgcacca gaccacgtcg gcatagggcg cataagccag gccgcgagcg atggccgaat
     1981 ccagcccgct gttgatgcga tagaatccct ccaccgtgcg ctcgccggtg cagaagggct
     2041 ggtcataggg atcgacgtcg ctggtcagca aatcggcccc gttggcgtcg gtgcgggcga
     2101 tgagaacgga cggcacgccc atgacgtcgg ccgccagccg cgccgccacc agcttctgga
     2161 caaactcgcg cgtgggcacg agcactttgc cgcccatatg gccgcacttc ttggccgacg
     2221 aaagttgatc ctcgaaatgg acgcccgccg cgccggcctc gatcatgccc ttcatcagtt
     2281 caaacgcgtt gagcgatccg ccaaagccgg actcggcgtc ggcgacgatc ggcgcgtacc
     2341 aataggtatc ttcttcatcc tgcaggtgat aaatctggtc ggcgcgctgc aaggcattat
     2401 tgatgcgctt caccagattg ggcacgctgt cggccggata gaggctctgg tcggggtagg
     2461 tatgggccga cgaattgttg tcgcccgcca cctgccagcc actgacatag atcgccttca
     2521 gccccgcctg cacctgctga acggcctgat tgcccgtcac cgcgcccagc gcggcgacaa
     2581 aatcctcctg ctgcatcagc cgccacaggc gctccgcgcc catgcgcgcc agcgtgtgct
     2641 caatctgcac cgaaccgcgc agccggatca catcctcggc cgaatagggc cggatgatcc
     2701 cctgccaccg cttgttcagt tcccaatcat ccttcaactt ggccacgtta tacggtttaa
     2761 acatcctcgc ctccacatcc tggttgttat tgtctgcggg gcgggtctat ggccgcaccc
     2821 caccgggttc acgtttcgtc ggagggcaga tggctccatc ccctgcgact taattcagat
     2881 attcgtaggc tttcaacgtc aggaaatcgg tgaattcatc ggcgacgctg atctcatcaa
     2941 acaacttggc agccagttca taatgccccg cggcgaactc ggtctcgcca acctggctct
     3001 tcaactccgc catgacctca tcggtcatct tgcgaaagag gccgaccttt atcgcgcggc
     3061 cgtcgtccag cttcgcgtgg gggttgtgca gccattgcca gatctgcgag cgggagatct
     3121 cggccgtggc ggcatcctcc atcaggttat agatgggcac gcagccgtta ccgctcagcc
     3181 aggcggccat gtagcggatg ccgacgtcga tgttggtgcg caagccgccg accgtgatcg
     3241 tccccgtggg aatagccagc aggtcggccg ccgtcacctc gacatcgccc cgcttgcgat
     3301 gaatctggtt gggcgtcggc atgtacttgt cgaatatctc gcgggcgatg ggaaccagcc
     3361 ccggatgggc cacccacgtg ccgtcatggc cgtccttggc ctcgcgctcc ttgtcggccc
     3421 gcaccttgcc cagcgcggcc tcgttggctt cgggatcatt cttgatcgga atctgggccg
     3481 ccatgccccc catggcgtgg atgttgcgcc ggtggcaggt cttgatgacc agttgggagt
     3541 aggcgcgcat gaacggcgtg gtcatggtca cctgggcgcg atcgggcagg tagaagtcgg
     3601 ggtgtttctg gaacttgcgg atggcgctga agatgtagtc ccagcggccg cagttcaacc
     3661 cggccgaatg atcgcgcagc tcccacagga tttcgtccat ctcgaacgtg gccaggatgt
     3721 tctcgatcag cactgtggcg cgaattgtgc cgttaggcaa gccgaggcgc tcctgggcca
     3781 taacgaagac gtcgttccac aggcgggctt ccaaatggct ctccagtttg ggcaagtaga
     3841 aataagggcc ggtctcgcgg gcgatgaggt tgcgagcgtt gtggaagaag taaaggccaa
     3901 aatcgaacag cgaggccgag atcggctcgc cgtccaccag catatgcttt tcgctcagat
     3961 gccagccgcg cgggcgggcc agcagcacgg ccgttcgctc gttcagcttg taggatttgc
     4021 cttcgggact ggtgaacgtg atggtgcgcg cgatggcgtc gcgcaggttg atttgcccct
     4081 cgacggtcgc cgcccaggtg ggagaatggg agtcctcgaa gtcggccatg tagaccgtgg
     4141 ccccggagtt gagggcattg atgaccatct tgcggtcgac cggcccggtg atctcgaccc
     4201 ggcggtcctg caagtcggcg gggatgggtg cgaccgtcca ctcggcggcg cgaatttcgg
     4261 ccgtttccgg caaaaagtcg ggcatcttgc cggcgtcgat ctctttctgc cgctccgcgc
     4321 gccgctgcag caaggccagc cggcggccgt taaaagcgcg ctgcaacccg gcaacgaatg
     4381 caagtgcctc aggcgtaagg atttccgcga attcgggcgt catctcgccg cggatatcga
     4441 ttccttcttg cgccatactg actctccttc caaacgcgat tgataacatt caccattagc
     4501 gaatattcgc taataatatt cgtagtatag agatttatgg tcagttttgt caatagctcg
     4561 ccggattaaa tttggctccc agcgaatatt cgctaaaata aggctgaaaa tagtggtttt
     4621 caagccattt aacgcattgc gtcattataa cgcattgtgt catgccttgt aaagggccaa
     4681 ttgagtattg gtggatgcgg gtatcgtaaa gaggtcaggc ctccagatcg cccccgcatc
     4741 cgcggagcaa catcatgagt ctcgatctcg tcaccatcat cttcggcatg aaagtgcgcc
     4801 aggcgcggct ggaagcaggt ctgacgctct ccgacctgtc ccgccagagc gagatgtccc
     4861 cctcctatct gacggagatc gagaaagggc gcaagtaccc tcgcgccgac aagatcgtca
     4921 agatggccgc ggccctcggc aaggagtacg acgatctcgt gtcgatcaag ctcgcccctt
     4981 cgctgcgcta cctggagagc acgctctcct catcgaccat tcgtcgcttc ccgttcgagg
     5041 agttcggcct ggagccggcc gacgccctgg ggcttgtgac gcgcgaaccc gaccgcgcca
     5101 gcgccctgct gcatgccgtc gtcgagatca cccgccgcta cgacctgcaa tcggaagagt
     5161 tcctgcgcgc cgcgctgcgc tcctatcagg aaatccacga gaattatttc cccgaacttg
     5221 aagaggcggc cgagcaattc atcgccaccg tgggcgcgcg ccatggcttc gacggcaaac
     5281 gattgatctc cctggcgacg ctggaaaaaa tcctgggcga cgagtacggc tatctcatcg
     5341 acgacaaggc catcgccgcc accgaggaac tgcgccatta ccgctcgctg gtcgtccccg
     5401 gccgcaagcc ccaactgttc atcaacagcc gcctctacgc gcgccaggtg cgcttcattc
     5461 tcgcccgcga gatcggctat cgttatctgg ggatcaagga gcgctcgttc gtctccacgc
     5521 ccgacgaggt ggactccttc cagcaactgc tcaacgacgc ccgcgcggcc tatttcggcg
     5581 gcgcgctgct catgccccga gagcatgtcc tcagcgacct gcggcaactc ttcgccatgc
     5641 cggcctggga cccggagcca ttctcggact tgctgtcgac ttacgacgtc acgccggaga
     5701 tgctgctcta ccgtttcagc gaactgatcc ccgaattctt cggcctcaaa atccacttcc
     5761 tgcgctttca tcacgcgacg ggcacggata attatcagct ggtgcgccat ctaaacctga
     5821 accaactcct cgtccccagc ggcaccgggt tgttcgagca ccactgccgg cgctggctct
     5881 ccctgcgtct gctgccggag ccggcagccg ggcggccatc atccggtgaa ccgctccccg
     5941 tgggcgtgca gatgtcggag ttcctggaga cgggcgacaa gttcctgtgc attggcttcg
     6001 gccggcgcat ggtcctgtca ccgggagtgg ccagcagcgt ggtcatcggc ttccgcgtgg
     6061 agccggagct gcgcgagacg atcgggttcg tcgacgatcc cgaaatcccc cacacgatca
     6121 tcaacgagac ctgcgaacgc tgcccgcttg gcccccagca gtgcgatgta cgcgccgcgc
     6181 ccccccatat attggaagcc gaacgagaac gcatggccag aaaaatggcg ctcaatcagc
     6241 taaaaaaccg gtcggcggct gcggaatgac ccttgatggc tgtggataac tggtgaaaag
     6301 ttgtggaaaa gtcgtattgg ttggggaaat ctggactctt tcccggttag tcgccgcagc
     6361 gtcattaatc tttatatggg tagttttcgc gacactcacg ccatatatgg gggtagaagc
     6421 cggaatggtt ccgggaagag cagaaatcag ggcgaaacgg tcgaaaaaga tatgccgcgg
     6481 gtctgcacag actatcccca cgcattgacc ggagaaaccg gcagataagg gggatacatg
     6541 gggtggattg gagcatggcc cccatatata ggccacctaa cgacttatcc agctttcccc
     6601 tgcccctact acatactact aataaaagaa caagggaaag gaaaccccat tggataatct
     6661 tctcttacaa aaaggacccc aacttggttg aagttggggg cctgacgtta cggcgtgatg
     6721 acgggaagga cagagacgtc catctccagt tgcagcgctt ccgcgcgtat ccagccctcc
     6781 tggccgcccg gaagccatat cttcacccat gccgcgtcct ctgtgcggcc gatcagcacg
     6841 acgctctgcc ccggcaccac ccgcaggatc ggcgccgtcc cctcatccgg cgtggcgtag
     6901 acgtagacga cgggcaatcc cggtggggtg gcgtccgtgg aggcttcctg agtcggcgcg
     6961 gccgtcccgg tgggcgaaac catcgtcgta gccgctgctg tccctgtcgc cgccggcgtt
     7021 cccgtggccg cgtttgttgc ggtggctccg gccgtcgcgg ttgctgttga cggcgggggc
     7081 gtcacggttg gtggcgccgt tgtggcggtc gcttgcgctg tggggacggc tgtggcaata
     7141 gactggattt tgctcggcgt ggtcgctgtt ggcacctgtg cctgcgaatc acccgctccg
     7201 gcgctcttgc tcccttgcca gcccaccacc tgcggcgcgg caatgccgtg taccaccggc
     7261 acattggcca gcgacgccga cacatggatg atctcggccg caatccagcc ctcgcggccg
     7321 tccgccagcc gtacctggac ccaatggttg tccgccgtgc ggccgatgat gcccagcgac
     7381 tgcccctcgg tcacgctgtc caccggctca tagaggaagc cggggccgtc gtagacgaca
     7441 aaatatgggg ccagatacgg cggcggcgcg tccaggaagg gggtcaggat gggcagatcg
     7501 ttgagcgttc ccgacggcag gaaggagcta tcggcgcgcg cccagccggg gcgcccgtcg
     7561 ggcaggcgaa catgcaccca ggtcgcgtcg tcgttgcggc cgttgagcgc cagcacctcg
     7621 cccggatgcg cccagccgac gaggccaaac cccgcgtccg cgccggtata gacggggatg
     7681 gggtcgttgg tgacgaacgc ggtgatgccc cacaggtcga cgtcggcgat aggcagggcg
     7741 gcgatgtcgg tcgtcgtgcg gatgtagctc gcgttgaccc accccagata gccgttatac
     7801 atggcgatct tcacccaggt gccggttcga ttgcggccga ccagcctgat ctcttcgcct
     7861 tccatcagat aggcgacggc cgtataactg acatgcggcc cggtgcgcag attgagcgac
     7921 tcggcgctga cacgcgcgcc gctcggcgtg gcagactgag atatgggcgg cggcccgctt
     7981 tgcgcctggc tgcgtcgtga atcgaccagc accagccccc ccagcatgag gagaatcagg
     8041 ccgataataa ataattttgt ccgcagacgt ggctcaaagg gtgtactcat ggcagaaccg
     8101 gaatatagga taatgtcaca ggggcttatg gaaactgcca aaccctctag ccttcagtat
     8161 atgagtggtc attgcaagtg ggcaaggttg tagcgcgatt ttgatctctt ttttcacacc
     8221 gtggtcgctg ttcacacctg gcgaaagcat ggggatcaac gctgcccgtt cgtaaaacag
     8281 gtgacccaag gctataataa tcctctgtgc tttcggaaaa gattacgacg gactggattg
     8341 acggcgcgcg ccagggaaac ccgcgcgcca ttgcgcgcct catcacgcgg gtggagagcg
     8401 atgctgcggc cgcgcaaacc atcatccgcg ccatctatgc ccataccggc caggcccata
     8461 tcgtcggcat caccggctcc cccggctccg gcaagagcag cctggtcaat gagctggcca
     8521 agagctttcg gcgcggcggc cgtcgcgtcg gcattgtcgc cgtcgatccc accagcccgt
     8581 tcaccggcgg cgcgattttg ggcgaccggg tgcgcatgcg cgatttatcg ggcgacgacg
     8641 ggatttttgt ccggtcgatg gcttcgcgcg gcagccttgg cggtctggct cgcgccacac
     8701 aggccgcggt aaagattctc gacgccgccg gctacgaagt catcctggtc gagacggtcg
     8761 gcgccgggca ggccgaagta gacatcgccc gcgccgccca taccaccgtt gtcctcgaag
     8821 cgccgggcat gggcgacgac gttcagtcta tcaaggccgg catcctggag atcgccgata
     8881 tcctggtggt taacaaggcc gaccagctgg gggccaatcg caccgtcaaa tcgctggaga
     8941 tgatgctcca tctggcgctg cccgccctta gcgacggaca tcatcataac gacggccatc
     9001 atcgcggcga tcctcacctt gctgagccgg ccggccgagc cacgccgggc tgggatattc
     9061 ccgtgctgcg aacgacggcg acgaccggcg cgggggtgga cgaactggcc gggatggtgg
     9121 atcgtcaccg cctgtattta aaggaatccg gcgggtgggc ggttcgcgag catgctcgcg
     9181 gcctggccga gatcgagcgg ctgttgcgcg atcggtttat cgacgagatg gtcgcccgcg
     9241 ttccgctcgc cgagcgggaa gcgctggttc gcgccgtggc cgagcgtcgc ctggatccct
     9301 atacggccgc tgaccgcctt ttccaggcca ccggcctatt cgcgtcgtaa tcagatttat
     9361 ttgagtgtgg agagttcatg ttcggcaata tcaaacttta tgccggttct gcctgccccg
     9421 agttggctca acgcatagcg gattacatgg gtattcgtct caatcgatgg gatctcatca
     9481 catttcccaa cgagaatatc ttcattcgtc tgaacagcag cgtgcgcggc caggatgtct
     9541 atctcatcca gacccacgac cggccggttc atgacaatat tatggagatg ctgatcgcca
     9601 tcgactgctt caagcgcgac tcggccgggc gggtgacggt cgtcattcct tacctctcct
     9661 attcccagag cgacaagaaa gaccagccgc gcgtgcccat caccgctcgc ttgctggccg
     9721 acatgatcga ggtggccggc gccgaccgct gggtgaccat cgatctccac gccgggcaaa
     9781 ttcagggctt ctacaagatc cccggtgact cattgaccgc gctccacatc atctgcaaat
     9841 atatccgcga gaaaagcctg gatgccacgg tggtgacgac cgacctgggc tatgccaaag
     9901 gcggccgcaa ttatgccgaa gcgctgggct tcccgctggc gttcgtggag aagcgacgcg
     9961 tgggcaacga aacggggcgc gaggcgctga ccctcatcgg cagcgtggaa ggcaaggatg
    10021 tgctgatcgt cgacgacctg gtggacacgg ccgggacggt ggagcaagcg gtgcaggttg
    10081 tgcgggagcg gggcgcgcgc gatgtctatc tggctttcac ccacgccgtc ctctccccgc
    10141 cggccgttga gcggctcgaa aggatgaata tcaaggagat cgtgacgacc gacacgctcc
    10201 ccattccccc cgagaagcgg ctgccgaata tgacggtgct gaccgtcgcc gaattactcg
    10261 gcgaggtgat cctgcgctcg cacgagggtc gcagcgtggg cgagctgttc aatgagtagc
    10321 ggttcgattt aacccgatcc ggccgaacgt caacaactta acgtatccct agggaaccgg
    10381 ttcctttgtg cgagattccc tataattgaa tgcgccatgt ttaagaaact tgtaaacagc
    10441 attgtcggtg atccgaacaa aaagataatc aataacctgc ggccgaccgt ctccgccgcg
    10501 agtgaattcg aatcgcggct gaagcagctg cccgatgagg ctttccgcga ccgcacggcc
    10561 gagctgaaag agcgcgtggc cgccggggcc gacctcgacg acgttctgcc cgaggcctac
    10621 gcgctcgtgc gcgaagcctc tcgtcgcgcc atcggcctgc gccattacga cgtgcagatc
    10681 atgggcggca tcctgctcac tcagggcgag gtcgtcgaga tgcgcaccgg cgagggcaag
    10741 acgctcgtcg ccacgctccc gctctatctc aatgccctgg ccggcgaggg cgctcatctg
    10801 gtgacggtca acgagtatct ggcccgccgc gacggcggct ggatgggcaa gatctttcac
    10861 ctgctcggca tgtcggtcgg cgtcatcggc ccgctgggtt tttcggccct gttcgacccc
    10921 gaatacgtca accccggcgc cgagctggaa gacgaacggc tggttcactg gcggccgagc
    10981 actcgccgtc aggcctacga ggccgatatc acctatggca tcagcagcga attcggcttc
    11041 gactatctgc gcgacaacat ggccgtgcgg caagaccagc tcgtgcagcg caatctccac
    11101 ttcgccatca tcgacgaggt cgacaacatc ctcatcgacg aggcccgtac gccgctcatc
    11161 atctccggcc ccgcggcccg ttccggcaag gattacgtcc gtttcgccga gtacgtgcgc
    11221 cggttgcgag ccaacaccgc cgacgaggaa gaggaaccca acggccacta cgacatcgac
    11281 gagaagagcc gcagcatcag cctgacggag atgggcatcg ccgaggtcga gaagcgcatc
    11341 gaggagatcg actccgaggc gggcgataac ctctatgacc cgcgtttcta ccacctgacc
    11401 tattacctcg ataatgcgct tcgagcgcaa tacctgttca agcgcgacgt ggactacgtt
    11461 gtccaggggg acgaggtggt catcgtcgat gacttcaccg gccgcctcat gcccggccgc
    11521 cgttattccg acggtctcca cgaggctatc gaggccaagg aaggggtcaa cgtcaagcgc
    11581 gagacggtga cggtcgccac catcaccctg cagaactatt tccgcctcta cgacaagctg
    11641 gccggcatga ccggtacggc gctgaccgac gccgaagagt tcgacaaaat ctacgaactg
    11701 ggcgtcacgc cgctgccgac caacgtcgaa tatatcatcg acaccggtca gatggggttg
    11761 gggacgcgcc gcgagaaggt cgatggagcc gaggccatcg tctacgtccg gccggagacg
    11821 ggcgagccgg tctacttcaa gcgcatcgac ttcgccgatc aggtgtacgg cacccttgat
    11881 gccaaggatc aggcgattct cagcgagatc aagaccgtcc acacttccgg ccggccggtc
    11941 ctcgtcggca ccacgtccgt cgagcactcg gagatgatct ccaactacct gcggcgcgaa
    12001 tccatcgccc acaacgtgct gaacgccaag attcatcaat ccgaagcgct catcgtggct
    12061 caggccggac gcaagggagc ggtcacgatc tcgaccaaca tggccggccg cggcaccgac
    12121 atcctgctgg gcggcaacgc cgaagggctg gcggccgaag cgctggagaa cgagatgttc
    12181 gaccggccga tgctgatcca gctggctttc aagctgctgg atgaaggcga ggaagcggcg
    12241 cgcgatacgg cccgcaaaag caacaagctg accgaagacc tggtcgacgc gctggtggag
    12301 acggaacacc ggttcgcgga ggccatggtc gagatcgatc aggtgcagat cgtcggccat
    12361 ctggcgcgtg tgctgcagga gccgtatggc atcgactaca acgacctgct tgaggtgttg
    12421 cgccgggttc gggccgggga actggccgag gcgcgcatct tcgtcgagag cctcgacaag
    12481 gacgtcgctc tggtcgacga catcatgcgt cgctggaacg aactcgtgcg ctaccagaac
    12541 atccagaccg acgagaaagc gacggcccaa ttcctggccg agcagacttt tgagcatcac
    12601 tataacgcgc gcgcggcgct catccgcgcc gtgctgggcg accggctcga cgaggccaac
    12661 aagctggtga cgacgatccc cggtctggcc ccccgccaca tcgcccgcat ccaggaactg
    12721 cgcgagcagg ccaagcgcga caaagaggag atttggcggc tgggcggtct gcatgtcgtt
    12781 ggctcggagc gacacgaatc ccgacgcatc gacaaccagc tgcgcggccg cgccgcccgt
    12841 cagggcgacc ccggctcatc gcgtttcttc ctgtcgctgg aagacgacct gatgcggcgt
    12901 ttcggcggcg agcggctgaa gggcttcatg tcccgcacca acatccccga agatatgccc
    12961 atcgagagcg ggttgctcga ccgcatcatt gaaagctcgc aggagcgcat cgaaggctac
    13021 aacttcgaca tgcgcaagaa catcgtcgaa tacgacgacg tgatgaacaa gcagcgccag
    13081 gcgatctaca acgagcggcg cgagattctc atgggcgagg acttcgacct cgatgagaag
    13141 gtggatgcag ctttcgatca ggccatcgag gagctgatcg atcattacgt gaacgattac
    13201 cccggtttca tccgcggcga agtcgagcgc gtggtgcagg acttcaccac cgacgcgacc
    13261 aacacggtca acgtggccgc cgtcctgcgc cgggtgggca gtctgctgcc actgatggcc
    13321 gaactcgacc gcgacgacct ggagcgcatg tcgcccgaca aactgacctc aacgcttgtc
    13381 tccctggccc atgagcaggg acggcagggc acgaacctgc tgcaactgct gcaagcgatg
    13441 agccgcttcc tgccgctggt gccgccggtg cccaatctgg gcgcgctggc ggcgcaaaga
    13501 ggcggccagg tgcagacgcg agaacgaatt cgcgtcgaat atctggccca tgtggagacg
    13561 cttttcaacg agtttctggc tgattacgcc gacaaggccg accgcgacgc catttggggc
    13621 gaggctcaga ccgagatcga tcgcgccttc tcccacttca acctcgatgg cctgtcgatt
    13681 aagaacaagg atgagcgcca ggcgcacttc cgcgagggtg tcgatggcgt gttgcgcgag
    13741 ctgctgctgc agagcctgtc ggcgctggac gccgatcaac tggccgaggc gctggaaggc
    13801 tacatcgaca agcagcagca gaagtggcgc aaatacatcg gcgaggaaag ctatcgcaac
    13861 tacgagcagt tgctcttgtt gcaggccatc gaccgcgaat ggcgcgacta tctgacggcg
    13921 atggacgacc tgcgacgaga gatcggcttg caggccttcg gccagcgcga cccgaagatc
    13981 gagtacaaga agcgctcgtt cgagatgttc ggcgacatgc gcaagaacat cgaccgcgat
    14041 attgccgacc gcttcttccg cgaaatctcc gggcatcagg ccttcatcca gcagcaacag
    14101 caggaagtag cctataaggt tcaatcacag gacgcaggct accagaccgt gaagcgcgag
    14161 aagggcaagg gcacggaact gcgcaaggac acgcccgacg tcggccgcaa cgacctgtgc
    14221 ccctgtggca gcggcaagaa gtacaagaac tgccatgggc ggccgggtat ggcgacggcc
    14281 gcggccgccg gtggcaatgg ccagagtgct cagccgtcga agagcggcaa gaagccgcag
    14341 cagcaaaaga agaaagcgcg gcgatagggt ttgcaatcga gcctcaggga ttcatcaaaa
    14401 ccctgaggct cttttttgtt tatcgctgac agcccgggca gaaatgggtg ctgcggccac
    14461 ccagcacgat gcgctcgatc ggcgtgccgc aggtatagca ggattgcccc gtgcgacgga
    14521 agacggccac cgcgttttgc atatcgccca tctccccgtc ggccttcacg tatgtgccga
    14581 tactggctcc ttcgcgctcg atgcccatcg ccagaacttt ttggatggcg gcgtggagtg
    14641 cggccgtctc g
//
LOCUS       DAOMQG010000043        14562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1790_length_14562_cov_10.499138, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000043 DAOMQG010000000
VERSION     DAOMQG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1790_length_14562_cov_10.499138"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLC06_04195"
     CDS             <1..473
                     /locus_tag="PLC06_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO89678.1"
                     /translation="MTLREELEAEQVRHLDLQRYCQVESGTTVRETIVRMRAAGGGTA
                     LITRNSELIGIFTERDALRRVAGEPQTLDEPVDDVMTARPITVLPDTSAADALWLMDD
                     HHFRNLPVIDRQGQIQGDMTYAAIIQYLATRYPVEVLNRPARPEQYPSKPEGGD"
     gene            584..2449
                     /locus_tag="PLC06_04200"
     CDS             584..2449
                     /locus_tag="PLC06_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRO89679.1"
                     /translation="MNENDYIIEERDSVVIRFAGDSGDGMQLTGTQFTNTSAVFGNDV
                     STMPDFPAEIRAPIGTLAGVSGFQVNFSNRDILTPGDAAQILVAMNPAALKASLSDVE
                     AHGTIIINTDAFSQANLRKAGYESDPRHDDSLKEYQIIEVPLTSLNREALRDIEGLST
                     KEKDRSQNFFALGLAFWMFDRSLDVTVDWINKKFAKTPPIREGNLRALETGYNFGETS
                     RIFQHRYRIRPASLPPGTYRKVTGNNAMAMGFVTAAQKMGKPLFYGTYPITPASDILH
                     ALAPLRNFDVRTFQAEDEIAAMGSVIGAAFGGALAVTGTSGPGIALKSEAMNLAVALE
                     LPMVIVNVQRGGPSTGLPTKVEQSDLLQAMFGRNGESPMAILAPSNPSDCFDIAIEAC
                     RLAVRTMSPVVILSDGFLANSSEPWMIPNADDIAPIEYSHPEGFHSNGSNGHEPFLSY
                     KRDPETMARPWAIPGTPGLEHRIGGLSKAPVTGNVSYNPLHHEQMVRDRADKIARLAE
                     IIPEQDVFGPPSGELLLVSWGGTFGSVRSAVAQAQRRGLSVAHAHLRYLNPFPRNLRD
                     ILGRYRQVIVPELNGGQLAFLLRGRFVLDIQSYTKVHGRPFTIAEISRKIEQMLG"
     gene            2533..3540
                     /locus_tag="PLC06_04205"
                     /pseudo
     CDS             2533..3540
                     /locus_tag="PLC06_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
     gene            3643..4086
                     /locus_tag="PLC06_04210"
     CDS             3643..4086
                     /locus_tag="PLC06_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO89680.1"
                     /translation="MPASTDIQDRLAKDTVELLAARAPVEVDAHTSLAKAIRQMNSHN
                     IGCVMVTDDSDKLVGIFTERDVLMRVIGVVDDMTTATVGQYMTPNPVAVRANLPIAHA
                     LHLMSVYSFRHLPLVDDDNRPTGIISFRDVVRHLRGTVESDAASN"
     gene            4180..6081
                     /locus_tag="PLC06_04215"
     CDS             4180..6081
                     /locus_tag="PLC06_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRO89681.1"
                     /translation="MSIDALEGEPLTQESLPDGDLIVNDFSIVVATANGTGSQTSNMA
                     ILRALFKMGIPVSGKNIFPSNIQGLPTWYHIRVSREGYTARRHTSEILVAFNPATVIQ
                     DIQTLPPGGICLYNGDWRSVPQRDDITYYAVPVNEFIRDTGMKGKLKDYISNMVYVGA
                     LAHLLDIPLEKLDDALLFLFKGKRRTADANMNVVRAAYEWAAANLPRNHPYRIAPMNE
                     TEGLIMMTGNEAGALGAVFGGVTFVGWYPITPSTSVIDSLESYLKQMRSDPETGETTY
                     AIVQAEDELAAIGMVVGAGFAGARAMTATSGPGISLMAEFVGLAYFAEIPAVIWDIQR
                     VGPSTGLPTRTSQGDITFTYYLGHGDTKNVILLPSSLRECFDFGYKAFDLADTLQAPI
                     FVLSDLDLGMNNWMSEPFEYPAEPIKRGKVLTAADVAERGFARYKDIDGDGIPWRTLP
                     GNEHPLAAYFARGTGHNEKAVYSERPEDWKNNMARLSHKFDTARNLVPGPVIDEIEGA
                     NIGIIAYGTTRPAIEEARDRLTADGVATSFMRLRALPINDEVKAFIERYDRIYVIEMN
                     RDGQVHSLLLTELPELATRLESLALLDGMPLTARWVVEAITAAEEDRPRRRQGTNGQD
                     NSVQWISVQ"
     gene            6225..7271
                     /locus_tag="PLC06_04220"
     CDS             6225..7271
                     /locus_tag="PLC06_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRO89682.1"
                     /translation="MTETTTPRAGKVNAIGLAQADYKGLPTTLCQGCGHNSIANQIVQ
                     VAYELNVRPAEVLKLSGIGCSSKSPAYFLGQSFGFNGLHGRMPSLATGALMGNHHMQV
                     IAISGDGDSASIGLGQFKHVLRRNVPMVYIVENNGVYGLTKGQFSATADEGQELKYAG
                     TNEFPPIDICMEAILAGCGFVARSFSGDAQQVRELMKAALSHRGTAVLDIISPCVAFN
                     NADTSTKSYHYGKENEVPLHGIGFVPKEEEIEIEAYDPGEMRIVELHDGSLLKLRKLE
                     TDYDPTDRMGAMHRLQWAEEKQELITGLVYYNDSRPNLVETSRMGATPLNALDDSRIR
                     PGREALATLMSELM"
     gene            complement(7372..8703)
                     /locus_tag="PLC06_04225"
     CDS             complement(7372..8703)
                     /locus_tag="PLC06_04225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRO89683.1"
                     /translation="MKKRFAISTWAIMLAILLALAACGPAISSEQAEQMTREAGGAGA
                     ETGAETETTTGTETEAASGAVEGPAVGVPPVEGKTTETDSGLKVTEILAGTGRSPEPG
                     DLVTMNIVGMLEDGTMFADTASAGEPIVATLTETDLFPGWYEGVLLMKEGGKARLTIP
                     PELAYGSEGAGGVIPPDATIIMDVELLEAKAPPVPTVVDDADLTETDSGLKYYDIAEG
                     EGDMPVSGQDVVVNYTAWLQDGNVYIASSDSVGGPLTFALDSDMGVFPGWNEGVATMR
                     PGGKRYLVIPPALALGEAGGGRVPPNSTLIMEVELVEVKPLVLPTEISEGDFTETASG
                     LRYYDIVVGDGPEATTGSTVTVNYTGWLTDNVKFDSSIDSGQPFPFTLGTGQVIPGWD
                     EGVAGMKVGGIRQLVIPADLAYGETGSGMIPPGATLIFEVELLGVQAASSD"
     gene            8878..11112
                     /locus_tag="PLC06_04230"
     CDS             8878..11112
                     /locus_tag="PLC06_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89684.1"
                     /translation="MIQRPVDRLISRGIVVAFLLIFFALALDTAGRKAITTDEPLHLT
                     HAIAMHQTGVMPIPEMHTPLTYRLISSLLGTEPPLPDITTLDTWPGQNPYAISRELLW
                     RADIHTDRIAWLGRFVVIGMGVILGALMGAWTLSLTRGNLPATGSLMLLYALSPNLLA
                     SAALATTDIAATLTWFLSIFTWWRYWQRPSWGRWLLAAISLGLALAAKLTGILLLPIT
                     FALALLYLRKTARWWRPLLIWAGMLPVAALVLWALYGFEIAGAVPMPAYVEAWRLLWH
                     EVDASHANYFWGSVTSVGSWFYLPATLLLKTPLLQLALFLLIPSVLWQERYRWRSLLF
                     LLLPAGFFLLVAAASRLNYGYRHALPAVPYLMVLSALGIPRLWARPISRVALVVGLGW
                     TALAALLIHPDHLTYFNELAGGRGYRYLGDSNLDWGQDLNQLADYAGRYQAETGKPLY
                     FSYTGTVQREHYGLIGPSLVEQFNQGDNTFSPANPPAGRYAINTGDLQGTGLILGELR
                     ESALFDWFRHREPLTMLGGSIFVYDVARQAEGGWIAHCLSPGPLLADPEAEQLIGRTS
                     LRHIRFDCNASWVFPENGAPGWYVLPLDAPLWINDWLKEPATEIVYRHQANAYGPGYA
                     IAYWPGSSPGSLFSDEIHRYTAAAGGPAALRAFGTRGREWLTIWQVGEATAEPLSIQA
                     HLISGGEAQVADGLGFSADQWLASDWFAQRHTFTTSGDTLETGLYNYLTLESVGPAIR
                     LTAP"
     gene            11240..13471
                     /locus_tag="PLC06_04235"
     CDS             11240..13471
                     /locus_tag="PLC06_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRO89685.1"
                     /translation="MSARLSRTLLALILIIYLLLALGYGIVTPLFETPDEHLHYFTAD
                     FIAREGRLPTTRDAGLMAQEAAQPPLYYVLASLIVRASGGAVDSPQLWPNPRSNPADP
                     SGESLAHPPLNVNVFIHGSAEAWPWRGYALSAHLLRALSALLGLGTLLCIYGAGRTVW
                     PGNPERALLAVALIAFLPQFAFIHGAVSNDAAITLFSTAAIWQLLRITNYELRITNGD
                     KSAIEGASLTSCVLLGLTIGLAMLSKTAGLLLLVWCVAVLAAWEWAGGGEHRLRRAFQ
                     AVVAVALPALTLGGWWLWRNWMLYGDPTATNQFVRLAGGLRTYTLYQVWLDMDRVWTS
                     LFARFGWMNVRPPAWIWIVWSAIVLAAAVGGLWGLMTTHRKRPTLTAHALFHPALILS
                     GWFILVAAAWLQFMLRTPADQGRLFFPALISAALGAAYGLSRWPRPWTQLLAVGLALL
                     TSIYSLAIVIPRAYATAPIVVALPVEAAPLDISFPEGLDLIGARVETAEARPGDWVWA
                     TLYWRARAERLSGAPLVYLELFGRGFRRIGLQTGYHGRGNFPSTLWPVGEIVADRVAV
                     RVTDDAETPVLAKLTVKLDEDEKGIDIGTVKVIPNEWPARMEPIALIGDGIELAAAEL
                     SSPAAAPGDTVEIRLRWQVAVPPGPALLHLFAHLGNPSAAPLAQTDGPVMGGEYPSSL
                     WVAGERFDETISLSLPADLPPGEYPVNIGLYDFATGARLPLVANGERTPTDAFTVGML
                     TIR"
     gene            complement(13487..>14562)
                     /locus_tag="PLC06_04240"
     CDS             complement(13487..>14562)
                     /locus_tag="PLC06_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRO89686.1"
                     /translation="IREGKIWIVNPAGQILPTPFLDIKDRVRFQDNFEQGLLGLAFHP
                     DYPRTPYYYIVYTVYDAIVVARGVVKPTSPNTADPLDLQVFIVIRKPDIPGDPSPGPS
                     PVHNGGDLVFGKDGYLYIPLGDGGPDPYSVPGPGDPNNNSQRRDTLLGSVLRIDPDPN
                     RGLKPDCGLANLYSIPRDNPYLNDDGCDEIWTYGLRNPWRMSIDPLNGDFYIADVGEW
                     LREEVNYVPANSPGGQNFGWHCWEGNVDYTLIHPELASKCKEQTNFTFPVFEYDHSHG
                     ECSIIGGKVYRGQKYPDLYGRYFFGDWCTGQLWTMYQNNGRWQVDEGGKQPLQFSTFG
                     EDIHGELYAGRYGNGTLYKVEVR"
BASE COUNT         2820 a         4769 c         4211 g         2762 t
ORIGIN      
        1 caatgacttt acgagaggag ttggaagccg agcaggtccg gcatctggat ctgcaaaggt
       61 attgtcaggt cgagagcgga acaaccgtcc gcgagacgat cgttcgcatg cgcgccgccg
      121 gcggcggcac ggcgctcatc acccgtaatt ccgagctgat cggcatcttc actgaacggg
      181 acgcgttgcg tcgcgtcgcg ggggagccgc agaccctcga cgagccagtc gacgacgtga
      241 tgacggcgcg ccccatcacc gtgctgcccg acacgtcggc cgccgacgcg ctatggctga
      301 tggacgacca ccattttcgc aatctgccgg tcatcgatcg ccaggggcaa attcagggcg
      361 acatgaccta cgccgccatc atccaatacc tcgccacgcg ttatcccgtt gaagtgttga
      421 atcgcccggc ccgaccggaa caatatccca gcaagccgga aggcggagat taggaattac
      481 gaattacgaa ttacgaatta cgaattagga attgaggacg accttttatt cggacaacac
      541 ctgtcatcat ttgtaattcg tcattcatca cttgaacaat gccatgaacg aaaacgacta
      601 cataatcgag gaacgcgatt cggtcgtcat ccgcttcgct ggagactccg gcgacggcat
      661 gcaattgacc ggcacccaat ttaccaacac ctcggccgtc ttcggcaacg acgtcagcac
      721 catgcccgac tttccggccg agatccgcgc ccctatcggc acgttggccg gtgtcagcgg
      781 cttccaggtt aacttctcca accgcgacat cctgacgccc ggcgacgcgg cccagattct
      841 cgtcgccatg aacccggctg ccctgaaggc cagtctgtcg gatgtggaag cccacggcac
      901 gatcatcatc aataccgacg ccttttccca ggccaatctg cgcaaggccg gctacgagtc
      961 ggacccgcgc cacgatgact cgctgaaaga gtatcagatc atcgaagtgc cgctcacatc
     1021 gctcaaccgc gaggcgctca gggacatcga agggctatca acaaaagaga aggatcgctc
     1081 gcagaacttc ttcgcgttgg gtctggcctt ctggatgttc gatcgctcgc tggatgtgac
     1141 cgtcgactgg atcaacaaga agttcgccaa gacgccgccc atccgcgagg gcaacctccg
     1201 cgcgctggaa accggctaca acttcggcga gaccagccgc atcttccagc atcgctatcg
     1261 catccgcccc gcctcgctgc cgccgggcac ctaccgcaag gtgaccggca ataacgccat
     1321 ggcgatgggc ttcgtcaccg ccgcccaaaa gatgggcaaa ccgctcttct acggcaccta
     1381 ccccatcacc cccgccagcg acatcctcca cgccctggcc ccgctgcgca atttcgacgt
     1441 gcgcaccttc caggccgagg atgagatcgc cgcgatgggt tcggtcatcg gcgcggcctt
     1501 cggtggcgcg ctggccgtca cgggaaccag cggccccggc atcgctctca agagcgaagc
     1561 catgaacctg gccgtggctc tggaactgcc gatggtcatc gtcaacgtcc agcgcggcgg
     1621 ccccagcacc ggcttgccga ccaaggtcga acaatccgac ctgctgcagg ccatgttcgg
     1681 ccgtaacggc gaaagcccca tggctatcct ggcccccagc aacccatccg actgcttcga
     1741 catcgccatt gaggcctgcc gcctggccgt gcgtacgatg agcccggtgg tcatcctctc
     1801 cgacggcttc ctggccaaca gttcggagcc gtggatgatc cccaacgccg acgacatcgc
     1861 gcccatcgaa tacagccatc ccgaaggctt ccacagcaac ggctcgaacg gccacgagcc
     1921 gttcctgtcc tacaagcgtg atcccgagac gatggcccgg ccgtgggcca tccccggtac
     1981 gcccggcctg gaacaccgca tcggcggcct gagcaaggcc cctgtgacgg gcaacgtctc
     2041 ctacaatcct ctccatcacg agcagatggt tcgcgaccgg gcggacaaga tcgcccggct
     2101 ggccgagatc atccccgaac aggatgtttt cgggccgccg tcgggcgagt tgctgctcgt
     2161 cagttggggc ggcacgttcg gctcggttcg ctcggccgtg gcccaggcgc agcggcgcgg
     2221 cctgtcggtg gctcatgctc atctgcgcta tctgaatccg ttcccgcgca acttgcgtga
     2281 cattctgggc cgctaccggc aggtgatcgt gccggaactc aacggcggcc agttggcctt
     2341 tctgctgcgc gggcgcttcg tgctcgacat tcaatcgtac accaaggtgc acggccggcc
     2401 gttcaccatc gccgagatca gccgcaagat agagcagatg cttggataaa acgaccgccg
     2461 gccgccggct gtcgaccgct tttctttcgg cggtcagcgg tcaacggtca acggtcaacc
     2521 agagagatag ccatgacaac cgataccatt cctgtccaaa aactcacccg ccaggatttt
     2581 gcttccgacc agacggtgcg ctggtgcccc ggctgcggcg actacgccat tctggcgacc
     2641 atccagaagg tgctgcccga gctgggcatc ccgcgcgaaa atgtcgtgtt catctccggc
     2701 atcggctgct ccagccgctt cccgtactac atgaacacct acggcatcca cagcatccac
     2761 ggccgcgccc ccacactggc cacaggcctg tcgatcgcca acccggatct atcggtctgg
     2821 gtcatcaccg gcgacggcga cggcctgtcc atcggcggca atcatatgct ccatgtgctg
     2881 cgccgtaacg tgaatctcaa cattatcctg ttcaacaatc gcatctatgg cctgacgaaa
     2941 gggcaatact caccgacctc tcgcctgggc gcgaagacca aatcgtcgcc gatgggcacg
     3001 atcgagcacc ccgtcaaccc gatcgggctg gcgctggcgg ccgaggcgac attcgtcgcc
     3061 cgcaccatcg acgcccacac ccagcacatg tacgaggtct tgcgccaggc agcccatcat
     3121 cgtggctcct cattcgtcga gatctatcaa aactgcgtca tcttcaaccc caccgaatgg
     3181 gagggcctgg aagatcggcg cacgcgcgac gacaacatcc tctacctgga acacggcaag
     3241 ccgatggtct tcggcaagaa ccgcgatcgc ggcattcgcc tgaacgggtt cacgccggag
     3301 gtagtcgctc tgggcgacgg catcaccgag gccgatctgg tggtccacga tgtcgaatcc
     3361 accgaattgg gctacgtatt gggcaatatg caatatccca ctcccatcgg cgttttccat
     3421 catgtgacga cctaccaaac ctacaccgac ggcctgatgg cccaggttgc cgaagcacag
     3481 gcgaagcgcg gcaagggcaa cctcaacgaa ctctatcgct cggctgacct gtggacggtc
     3541 gtgccgcagg aacctgatga gcctattcag caccacatct ccgaccgcgt ggccgatgaa
     3601 ctggatgacg aatacgtcga tcggctggct ttcggcgagg aaatgcccgc gtcgacggat
     3661 atacaggatc ggctggccaa ggataccgtg gagctgcttg ccgcccgcgc gccggttgag
     3721 gtcgacgcgc acacatccct ggccaaggcc attcgtcaga tgaacagcca caacatcggc
     3781 tgtgtcatgg taacggacga cagcgataag ctggtcggca tcttcaccga gcgcgacgtg
     3841 ttgatgcgcg tgatcggggt cgtcgatgac atgaccacgg ccacggttgg ccaatacatg
     3901 acgccaaacc cggtcgccgt ccgcgccaac ctgccgatcg cccacgcgct gcatctgatg
     3961 tcggtctaca gcttccgtca cctgccgctg gtggacgacg acaaccggcc aaccggcatc
     4021 atctctttcc gcgacgtggt tcgccatctg cgcggaacgg tcgagagcga cgccgcgagc
     4081 aactaacgcc cgccaatcgt cgatcatgaa cgcataaatt tctcataggc tgtgatgagg
     4141 tcatcacagc ctgtcattgt tctgaaggag aataacgtaa tgagtattga tgcacttgag
     4201 ggagagcccc tcacgcagga gagcctgccc gacggcgacc tcatcgtcaa cgatttctca
     4261 atcgtcgtgg cgacagccaa cggaaccggc agccagacct ccaacatggc catcctgcgc
     4321 gccctgttca aaatgggcat tcctgtcagc ggcaagaaca tcttcccgtc gaacattcag
     4381 ggattgccga cctggtacca cattcgtgtc agccgcgagg gatacaccgc ccgccgccac
     4441 acgtcggaga tcctggtggc cttcaatccg gcgaccgtca tccaggacat ccagacgctg
     4501 ccgccgggcg gtatctgcct ctataacggc gactggcgct cggttccgca gcgcgacgac
     4561 atcacctact atgccgttcc ggtcaatgaa ttcatccgcg acacgggcat gaagggcaag
     4621 ctgaaggact atatctccaa catggtctac gtcggcgcgc tggcccattt gctcgatatc
     4681 ccgctggaga agctggatga tgcgctgtta ttcctgttca agggcaagcg ccgaacggcc
     4741 gacgccaaca tgaacgtcgt gcgcgcggct tatgagtggg cggcagccaa cctgccccgg
     4801 aatcatccct atcgcatcgc gccgatgaac gagaccgagg ggctgatcat gatgaccggc
     4861 aacgaggccg gcgcgctggg cgccgtcttc ggcggcgtca ccttcgtcgg ctggtacccg
     4921 attacccctt caaccagcgt catcgactcc ctcgaaagct atctgaagca gatgcgcagc
     4981 gaccccgaaa cgggcgaaac gacctacgcc atcgtgcagg ccgaagacga attggcggcc
     5041 atcggcatgg tcgtcggcgc gggcttcgcc ggcgcgcgcg ccatgacggc gaccagcggc
     5101 cccggcatct ccctgatggc cgaattcgtg ggcctggcct atttcgccga gatcccggcc
     5161 gtgatctggg acatccagcg cgtcggcccc agcaccggcc tgccgacccg caccagccag
     5221 ggcgacatca ccttcaccta ctacctgggt cacggcgaca cgaagaatgt catcctgctg
     5281 ccgtcgtcgc tgcgcgaatg ctttgacttc ggctacaagg cgttcgacct ggccgacacg
     5341 ctccaggcgc ccatcttcgt cctcagtgac ctggacctgg gcatgaacaa ctggatgtcg
     5401 gaaccgttcg aatatccggc cgagccgatt aagcgcggca aggtgctgac ggcagccgac
     5461 gtggccgagc gcggcttcgc ccgctacaag gacatcgacg gcgacggtat cccctggcgc
     5521 acgttgcccg gcaacgaaca cccgctggcg gcctacttcg cccgcggcac agggcacaac
     5581 gagaaggccg tctatagcga gcgccccgaa gactggaaaa acaacatggc tcgcctgtcg
     5641 cacaaattcg acaccgcgcg caatctggtt cccgggccgg tcatcgacga gatcgagggg
     5701 gccaatatcg gcatcatcgc ctacggcacg acccgcccgg ccatcgagga agctcgcgac
     5761 cggctgacgg ccgacggtgt cgccaccagc ttcatgcgcc tgcgcgccct gccgatcaac
     5821 gatgaagtca aggcgtttat cgagcgttac gaccgcatct acgtcattga gatgaaccgc
     5881 gacggccagg tgcacagcct gttactcacg gagctgccgg aactggccac gcgcctcgag
     5941 tcactggcgc tgctggacgg catgccactg acggcgcgct gggtggtcga ggccatcacc
     6001 gctgcggaag aggatcggcc gcgccgccgc cagggaacca acggtcagga caacagtgtt
     6061 cagtggatca gtgttcagta gatcagtatt cagtagatca gtattcagta gatcagtgtt
     6121 cagtagatca gtcgatcact gacccactga cctactgacc tactgaccca ctgatctact
     6181 gactaacaga ctcgctattt acataaatac ggagtgcaac tatcatgacg gaaacaacaa
     6241 caccgcgcgc gggcaaggtc aacgccatcg gcctggccca ggcggactat aaaggactac
     6301 ccacgacgct atgtcagggc tgcggccaca actctatcgc caaccagatc gtgcaggtgg
     6361 cctacgaact taacgtgcgc ccggccgagg tgctcaaact gagcggtatc ggctgttcca
     6421 gcaaatcacc ggcctatttc ctgggccaga gcttcggctt taacggactc cacggccgca
     6481 tgccttccct ggcgaccggc gcgctgatgg gcaaccacca catgcaagtc atcgccatca
     6541 gcggcgacgg cgactcggcc agcatcggcc tggggcagtt caagcatgtg ctgcgccgca
     6601 atgtacccat ggtctacatc gtggagaaca acggcgtcta tggattgacc aaaggccagt
     6661 tctcggccac ggccgacgaa gggcaggaat tgaaatacgc cgggacgaat gaattccccc
     6721 ctatcgacat ctgcatggaa gcgatccttg ccgggtgcgg cttcgtggct cgctccttct
     6781 ccggcgacgc ccagcaggtg cgcgaactga tgaaagcagc cctcagccat cgcggcacgg
     6841 ccgtcctcga catcatcagc ccgtgcgtgg ctttcaacaa cgcggacacc tccaccaaga
     6901 gctaccatta tggcaaggaa aatgaggtcc cgctgcacgg tatcggcttt gtgcccaagg
     6961 aagaggagat cgaaattgag gcctacgatc ccggagagat gcggatcgtc gagctgcacg
     7021 acggctccct gctcaagctg cgcaagctgg agacagacta cgatcccacc gaccggatgg
     7081 gagcgatgca ccggctgcaa tgggcggaag agaagcagga gctgatcacc gggctggtct
     7141 actacaatga ttctcggccg aacctggtcg agacatctcg catgggcgct acaccgctca
     7201 acgcgctgga tgattcgcgg atacggccgg ggcgcgaggc gctggcgacg ttgatgagcg
     7261 aactgatgta gatcaaaaaa ccgggtttct cggaagaaac ccggttttta ttcgtcgatt
     7321 acgaaaatat agacgggccg taacgacgaa tcgttacggc ccgtttgtta tttaatcgct
     7381 cgacgctgcc tgcacgccca gcaactcgac ctcaaaaatg agggtcgcgc caggggggat
     7441 catgccggag ccggtctcgc cataggccaa atcggccggg ataaccaact gacggatgcc
     7501 gccgaccttc atgcccgcaa cgccttcatc ccagccgggg ataacctgtc cggtgcccag
     7561 cgtgaacggg aaaggctgac cgctgtcaat cgagctgtcg aacttgacgt tgtcggtcag
     7621 ccagccggtg tagttcaccg tcacggtgct gccggtcgta gcctccgggc catcgcccac
     7681 gacgatgtcg taatagcgca ggccgctcgc ggtctcagtg aaatcgcctt cggagatctc
     7741 cgtcggcaga accagcggct tcacttccac cagctcgact tccatgatca gcgtcgagtt
     7801 cggcggaacg cggccgccgc ctgcctcgcc caaagccagc gcgggcggaa tgaccaggta
     7861 acgcttgccg ccgggccgca tcgtcgccac gccttcattc cagccgggga agacgcccat
     7921 atcgctatcc agcgcaaacg tcagcggccc gcccacagag tccgaggaag cgatgtagac
     7981 attgccgtcc tgcaaccagg cggtgtaatt cacgacgacg tcctggccgg acacgggcat
     8041 atcgccctcg ccctcggcga tgtcgtagta tttcaggccg ctgtcagtct cagtcaggtc
     8101 ggcgtcatca acgaccgtcg gcaccggagg agccttggcc tccagcaact caacgtccat
     8161 gatgatggtc gcatcgggag gaatgacgcc gcccgcgccc tcggaaccgt aagcgagttc
     8221 gggcggaatc gtcaggcgag ccttgccgcc ctctttcatc aggaggaccc cttcatacca
     8281 accggggaaa aggtcggttt ccgtcaacgt agcgacaatc ggttcgccgg cgctggcggt
     8341 atcggcaaac atcgtgccgt cctcgagcat gccgacaatg ttcatggtca caagatcgcc
     8401 aggttcaggc gagcggccgg tgccggccaa aatctcagtc accttcaggc cgctgtcggt
     8461 ttctgtggtc ttcccttcaa ccggggggac gcccacggca ggtccttcaa cggctccgct
     8521 ggcggcctcc gtctcagtgc cggtcgttgt ttccgtctcg gcgccggttt cggctccggc
     8581 tcccccggct tcgcgggtca tctgctcggc ttgttccgag gaaatagccg gcccacaagc
     8641 agccagcgcc aataaaatcg cgagcatgat cgcccacgtt gaaatcgcga atctcttctt
     8701 catgtgtaac gtttcaacac tccttctatg gaaaattcca gttgccggtg gtgtagccgg
     8761 caaagtctgt gcacagtcta ttaagtctaa ctccgcctgc gcgcgcgcgc caatttcccc
     8821 tagccgtctc tcagaaattg aggccgcgct cgccccaccg atgatataat ggccgccatg
     8881 attcaacgtc cggttgatcg tctcatctcg cgggggatcg tcgtcgcctt cttgctgata
     8941 ttcttcgcgc tggccctcga cacagccgga cgcaaagcca tcaccaccga cgagccgctt
     9001 catcttaccc acgccatcgc catgcaccaa acgggcgtca tgcccatccc ggagatgcac
     9061 acccccctca cctaccggct catcagcagc ttgctgggca ccgaaccgcc cctgcccgac
     9121 atcacgacgc tggatacctg gccagggcaa aatccctacg cgattagccg cgagttgctc
     9181 tggcgggccg atatccatac cgaccggatc gcctggctcg gccgtttcgt cgtcatcggc
     9241 atgggggtca tcctgggcgc gttgatgggc gcctggacgc tgtctctgac ccgcggcaac
     9301 ctgccggcca cgggcagcct catgctgcta tacgccctgt ctcccaacct gctggcctcc
     9361 gcggccctgg cgaccaccga catcgccgcc acgctgacct ggtttttatc catcttcacc
     9421 tggtggcgtt attggcagcg gccaagttgg ggacgctggc tgctcgccgc catcagcctg
     9481 ggtctggcgc tggccgccaa gctgaccggc atcctcttgc tgccgattac cttcgcgctg
     9541 gccttgctct acctgcgcaa gaccgcgcgt tggtggcggc cgctgctcat ctgggccggg
     9601 atgctgcccg tggctgcgct ggttctgtgg gcactctatg gtttcgagat agcgggcgcg
     9661 gtgccaatgc ccgcctacgt agaggcctgg cgcctgctgt ggcatgaagt ggatgccagt
     9721 cacgccaact acttctgggg cagcgtgacc tcagtcggct cgtggttcta tctgccggca
     9781 accttgctgc tcaagacgcc tttgctgcaa ctggcgttgt tcctgttgat cccatccgta
     9841 ttatggcagg aacgatacag gtggcggtcg ctccttttcc tgcttttgcc ggccggcttt
     9901 ttcttgcttg tagctgccgc cagccgcctg aattacggct atcgccacgc gctgccggcc
     9961 gtgccctatc taatggttct cagcgcgctg ggcatccccc gattatgggc gcggccgatc
    10021 tcccgcgtgg cgctggtcgt ggggttgggc tggacggcgc tggcggcgct tctgattcac
    10081 cccgatcacc tgacctattt caacgagctg gccggcggcc gtggctatcg ctatctcggc
    10141 gattcaaacc tcgactgggg gcaagacctg aatcaattgg ccgattacgc tggccgctat
    10201 caagcagaga cgggcaagcc actctatttc tcctacacgg gaaccgtcca gagagagcat
    10261 tacgggctga tcggcccatc gttggtcgag cagttcaatc agggagacaa cactttctcc
    10321 ccggcgaacc ccccggcggg ccgctatgcc atcaataccg gcgacttgca gggcaccggc
    10381 ctgatattgg gcgaactgcg cgagagcgcc ttgttcgact ggttccgcca tcgcgaaccg
    10441 ctaacgatgc tgggcggctc gatcttcgtc tacgatgtgg cccggcaagc ggaagggggc
    10501 tggatcgccc attgcctgtc gccggggccg ctattggccg atcccgaagc cgagcaattg
    10561 atcggccgaa ccagcctgcg tcatattcgg ttcgattgca acgccagctg ggttttcccg
    10621 gaaaatggcg cgccgggctg gtatgtgctg ccgctcgatg cacccctgtg gatcaatgat
    10681 tggctaaagg agccggcgac cgaaatcgtc tatcgccatc aagcgaacgc ctatgggccg
    10741 ggctatgcta tcgcctattg gccgggatca tcacctggct cgctgttctc cgacgagatt
    10801 caccgttaca ccgccgcagc cggcggcccg gcagcgctgc gagcgttcgg aacgcgcggc
    10861 cgggaatggc tcacaatatg gcaggttggc gaagcgacgg ctgaaccctt gtcgattcaa
    10921 gcccacctca tctccggcgg cgaagcccag gtggctgatg ggctgggatt ctcggccgat
    10981 caatggctgg caagcgactg gttcgcgcaa cgacacacct tcaccacatc gggcgacacg
    11041 ctggaaacgg gcttgtataa ctatctgacg ctggaaagcg tcgggcctgc aatccggctc
    11101 acggcaccat aaccgatccc tggctaaccg ttcatctgcg ccgttcaact cctgtttccc
    11161 cggttgcccg gcaaattttg gttgagacgg gcgcgagtga taccattacc acgcttgaaa
    11221 gagcgagacg ctattcaata tgtctgcccg cctgtcccga acgctcctgg ccctgatcct
    11281 gatcatctat ttactcctgg ccctggggta tggaatcgtc accccgcttt tcgagacccc
    11341 cgatgagcac ctgcactact tcaccgccga cttcatcgct cgcgagggac gactgcccac
    11401 gacgcgcgac gccggtctca tggcccagga agcggcccag ccgccgctct attacgtgct
    11461 tgcgtcgcta atcgttcggg cgtccggcgg cgcggtcgac tcgccccaac tgtggcccaa
    11521 tccacgctcc aaccccgccg acccatccgg cgaaagcctg gcccacccgc cgctcaacgt
    11581 caatgtcttt atccacggct ccgccgaagc ctggccgtgg cgcggctacg cgctatcggc
    11641 tcatctgctg cgcgccctgt cggcccttct gggactgggg acgctgctgt gcatctatgg
    11701 cgcgggacga acggtttggc cgggcaatcc ggaaagggcg ttattggcgg tggcgttgat
    11761 cgccttcctg ccccagttcg cctttatcca cggcgcggtg agcaacgacg cagctattac
    11821 acttttcagc actgcggcga tctggcaact gttacgaatt acgaattacg aattacgaat
    11881 tacgaatggg gataagagcg ctatcgaggg cgcctcgctg acttcctgtg tgctgctggg
    11941 gctaacaatc ggcctggcca tgctctccaa gacggccggg ctgttgctgc tggtctggtg
    12001 cgtggcggta ttggccgcgt gggaatgggc tggaggcggc gagcatcgct tacggcgggc
    12061 gttccaggcc gtcgtagcgg tggctctacc cgccctgacg ctcggcggct ggtggctctg
    12121 gcgcaattgg atgctttatg gcgaccccac ggccaccaac caattcgtgc gcctggccgg
    12181 cgggctgcga acctacactc tctaccaggt gtggctcgac atggatcgcg tctggacttc
    12241 gctcttcgcc cgtttcggct ggatgaacgt ccgcccgccc gcctggatct ggatcgtgtg
    12301 gagtgccatt gttcttgccg cagccgtagg tggcttgtgg ggcctgatga caacacatcg
    12361 aaaacgcccc accctaaccg cccacgcact attccatccc gcgctcatcc tgtccggctg
    12421 gttcatcctg gtcgccgcgg cctggttgca atttatgctg cgcacaccgg ccgatcaggg
    12481 acgactattc ttccccgcgc tcatctctgc ggcactgggc gctgcttatg gcctgagccg
    12541 gtggccgcgg ccatggacac agctcctggc cgtcggcctg gcgctgctga ccagcatcta
    12601 ttcactggcg atagttatcc cccgcgctta tgccaccgcg cccatcgtcg tcgctctccc
    12661 ggtcgaggcg gcgccgctgg atatctcctt tcctgaaggg ctggacctca tcggcgcgcg
    12721 cgtggagaca gccgaggctc gcccgggcga ttgggtatgg gctaccctct attggcgggc
    12781 acgagcggaa cgcttatccg gagcaccgct ggtctatctg gagctatttg gccgcggctt
    12841 ccggcgtatc gggctgcaaa ccggctacca cggccgcggc aacttcccaa gcaccttatg
    12901 gccggttggc gagatcgttg ccgaccgcgt ggccgtgcgc gtcaccgacg atgcggaaac
    12961 gcccgtcctg gcgaagctga ccgtcaagct ggatgaggat gaaaagggga tcgatatcgg
    13021 cacggtgaag gtgataccca acgaatggcc ggcgcgcatg gagccgatcg cgctcattgg
    13081 agacggcatc gaactggccg cggccgagct gtcctcgccg gcggccgcgc cgggtgatac
    13141 ggtggagata cgcctgcgct ggcaggtggc cgtgccgccc ggccccgcct tgctccacct
    13201 cttcgcccat ctgggtaatc cttcggccgc cccgctggcg caaaccgacg gcccggtcat
    13261 gggtggcgaa tatccgtcaa gcctgtgggt ggcgggcgag cgatttgatg agacaatttc
    13321 gctgtcgttg ccggccgatc tgccgccggg cgaatatccc gtcaatatcg gcctctacga
    13381 cttcgccacc ggcgcgcgcc tgccgctcgt cgccaatggc gaacgcacgc ccacggatgc
    13441 cttcaccgtg ggcatgctga caatccgcta gcgatccgcc cgcgcgtcag cgcacctcaa
    13501 ccttgtagag cgtgccgttc ccatatctgc cggcatacag ttcgccgtgg atatcctcgc
    13561 caaatgtgct gaattggagt ggctgtttcc cgccctcatc cacttgccac cggccgttat
    13621 tctggtacat cgtccacaac tggccggtac accagtcgcc gaagaaatag cgaccataaa
    13681 ggtcggggta cttttggccg cgatagacct tgccgccaat aatggagcac tccccatgtg
    13741 aatgatcata ctcgaacacg ggaaaagtga agttcgtctg ctccttgcat ttcgacgcaa
    13801 gctcggggtg gatcaacgta taatccacat tgccttccca gcaatgccag ccgaagttct
    13861 gaccgccggg gctattggcc ggcacatagt tgacttcttc acgaagccat tcaccgacat
    13921 cggcgatata gaaatcgccg ttcaatggat cgatggacat tcgccagggg tttcgcaagc
    13981 cgtaggtcca gatctcatca cagccgtcgt cgttgagata cggattatca cgtgggatcg
    14041 agtagaggtt ggccagcccg caatccggtt tcaggccacg attgggatcg ggatcgatgc
    14101 gcagaacgct gcccaacagc gtgtcccgtc gctgcgaatt attattagga tctcccggcc
    14161 ccggcacact gtaaggatcg gggcctccat cacccaaagg gatgtacaga tagccatcct
    14221 tgccgaatac cagatcgccg ccattatgga cggggctggg gccgggggac ggatcacccg
    14281 ggatatccgg cttcctgatg acgataaaca cctgcaaatc aaggggatcg gcggtattgg
    14341 gcgaggtcgg cttaacgaca ccacgcgcaa ctacgatcgc atcatagaca gtgtaaacga
    14401 tgtagtaata gggtgtcctg ggataatcag gatggaacgc caggcccagc aacccctgtt
    14461 caaaattatc ctgaaaacgc acccgatcct tgatatccaa aaacggcgtt ggcaatatct
    14521 gcccggcagg gttaacgatc cagattttgc cctcgcgaat ag
//
LOCUS       DAOMQG010000044        14465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1807_length_14465_cov_10.712561, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000044 DAOMQG010000000
VERSION     DAOMQG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_14465_cov_10.712561"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            489..1205
                     /locus_tag="PLC06_04245"
     CDS             489..1205
                     /locus_tag="PLC06_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO89687.1"
                     /translation="MLSLDAQNKWRDEYRKRHPGWRPATEVFAEQVHENLRPHSRLLD
                     VGCGRGGLVEQLEHPLSQAVGIDPDFFSLAEHRLENLPRAVALSDHLPFVNDVFDIVI
                     ASWLLEHLPNPVSTFQAIGRVLRPGGVFIFITPNKRHPLTVANRFVGRLGSLQGRLVA
                     RLYGRAPEDTFSTFYRANTATELHDLTTSAGLVTESITMIADPSYVAFNRLLFHGMSA
                     IDKGLPQDRQIHIVGVARKI"
     gene            1358..>1542
                     /locus_tag="PLC06_04250"
     CDS             1358..>1542
                     /locus_tag="PLC06_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89688.1"
                     /translation="MESLSPLRRAYYNYLSRAYAMMYRLHKRSLLGIRLVSIARWLPI
                     LVLIIGWRQRWPLPALIG"
     assembly_gap    1543..1642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1836..2561
                     /locus_tag="PLC06_04255"
     CDS             1836..2561
                     /locus_tag="PLC06_04255"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO89689.1"
                     /translation="MNESEALLVVERGPVPTTQIQLQNDQFTVGRSAGNDLVLADPEV
                     SRRHIRVVRRADGHAVEDIGSTNGTFVNGQRISHLTLLQDGDAIDLGDTVRLRYMSLA
                     PAEDDDDTGPLGPTAEKPTQAYTPPAAPAEERPVERPQPIAPVAHKPAIAPVAHKPAV
                     TPTLPPPTPAYPPPPTYAHEEPRRGRGLWIGCGLLVALLLVCAGTFLVLDAYDQGRLL
                     YCGPLAPMFQIILGPFGFAPICS"
     gene            2583..3812
                     /gene="amrB"
                     /locus_tag="PLC06_04260"
     CDS             2583..3812
                     /gene="amrB"
                     /locus_tag="PLC06_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRO89690.1"
                     /translation="MPVSEKPQLRVLDFQPVFHRGERMWLLRDPWQLSDRQLIVPQAI
                     AQMLLLCDGTRTAAELRAEIAEELGIDIPFEVVRDALSQLDQVYLLHNERFEAQHAHA
                     LAEYRAHPYRPPALAGLGYPASPAELITQFESYGNATQGRDPVSQAWRGVVSPHIDYQ
                     RGGPVYAQVWQEAKSAVLDAELVLIFGTDHNGSAGSITLTHQPYATPFGVLPNDMDLI
                     NKLAAAIGEESAYAEELNHRNEHSIELSAVWLHYIFHQAKRAPCPMIPILIGSFHHFL
                     SNGHHPATDPTFHRFWDTLKQGTAGRRVLAVGSVDLAHVGPAFGDSFIMDAPRRMSLR
                     EEDRGLTSAITRGSAAEFYDRIFAVHDRNRICGFAPLYHMLSYLNATTGTRIAYDQCP
                     ADQMDTSLVSICGLLLQ"
     gene            4009..4221
                     /locus_tag="PLC06_04265"
     CDS             4009..4221
                     /locus_tag="PLC06_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO89691.1"
                     /translation="MNKETLLESYSDVDQVSKRLGIHPESVRRLIRDGKLPAIKFGNK
                     WLVEKSVLEQFASGYDGRPGNKATLF"
     gene            4285..5421
                     /gene="ftsZ"
                     /locus_tag="PLC06_04270"
     CDS             4285..5421
                     /gene="ftsZ"
                     /locus_tag="PLC06_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRO89692.1"
                     /translation="MQQTTNTDCSASIRVVGVGGAGSNAVNRMIAEGVKDVDFVVINT
                     DNQALSLSDAPVRVRIGDKLTRGLGAGGFPDQGEKAANESIEEIQSVLQGSDMVFVTA
                     GMGGGTGTGAAPVVARVAREMGALTIGVVTRPFHFEGSRRATSAERGIEQLQKHVDTL
                     IIIPNDRLLDITDKRMPLHESFHLADDVLRQGIQGITELITVPGLINLDFADVKTIMT
                     QGGAALMAVGRGTGEDRARIAAEQAVSSRLLDVTIDGAQGVLFNITGGTTLSLYEVNQ
                     AAEVIRETTHPDANVIFGAVIDETMGDEIRITVIATRFDQVHMRRQMLQRNDTGLFET
                     AARQAQSKERAERKPEPAQEQVEQEATPRFAPGNLEVPAFLRRK"
     gene            complement(5532..5951)
                     /locus_tag="PLC06_04275"
     CDS             complement(5532..5951)
                     /locus_tag="PLC06_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO89693.1"
                     /translation="MGDRVHIGLGLLALLFSSLACNAFAGRANTLPPPPIATATSSVG
                     ESSTEPNFAPTATLPADTPMEDVQGSLRILVDLNIRSGPGVSYDRVGFLLRERRVTVI
                     GRDPQSGWWRIQCPEDAEGDECWVSGGSQFTLLESTD"
     gene            6087..7397
                     /locus_tag="PLC06_04280"
     CDS             6087..7397
                     /locus_tag="PLC06_04280"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRO89694.1"
                     /translation="MSFRGFIETAADNGDLVTIDKAVDTTFEVANVAHALEGRPVLFT
                     NIQNYPGWRICSGVCADRKFFSMALGVEVPDLLHHLKDSLENPVPPPVVGSGSCQEVI
                     VENVNLNDLPILLHLPQDGGHYIASNVVIVNDPELGRNMCYHRLLRLDERRFAARIVE
                     NRGTFNAMQKTEGDLPVAICIGVSLGVHLAASMSPPPGVDELAVANALAPTPLVKCLT
                     NDLYVPADAEIVLEGRITKGLYSEGPFMDLTETMDITRMQPIIEIDLITHRQDPIFHA
                     LLPGGLEHKLLMGMPKEPTIFAAVNQVTRCTGAVITPGGASWLHAVVQIDKQGPDDGK
                     LAIEAAFKGHGSLKHVWVVDTDIDLYDPAQVEWALATRFQADRDLYVYANQPGSSLDP
                     SGTHVPGRKSLTAKMGLDCTIPWGADRSMYERGEYGVVDLSEYL"
     gene            7501..9291
                     /locus_tag="PLC06_04285"
     CDS             7501..9291
                     /locus_tag="PLC06_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HRO89695.1"
                     /translation="MLNDEQRAMLAGEQGRARQMGMRLLLDMAAMAGAEHLSPIGSAH
                     LSGVSPLTGGLGLRQFLARLAEDPGEGVAIPTTLNAAGCDEDQFEAMRIVVPGFLDHH
                     REIIKSYAGLGVQTIQSCVPYEWDEVQSRLTQDMPAAWAESNAICFANSYSGLRTNRE
                     SGLSALACALTGYAPNYGLLEDEGRRPNLAVTVTVDLEDPTDFSVLGDWIGKQRRPNW
                     RMPFGPIPAIIGLPATLTHEQRKAMSAAAANYGSPLLHIIEGDEFPEAEYQDRLTFGR
                     TELEQRYAELKPKKPVSLVVLGCPQASIGELKAIAGYLRGRRVTADPSGPGEPPPLWI
                     FTSAITKAVAERTGLADIFRKSGALFLENTCPEVVPYDPGWVNHVLTNSMKAEHYIKS
                     GLNGIPTSVMRLADCVAIATGDMVLSYEDAHRMTTTASGKKNKTQETKSSGATAEQSA
                     QAASAVAVGQGLPSQGDFTVIGEAFVTDTPITFLGYVNRETGVIEEEDHPANGQSMAG
                     KIAIFPRGTGSSVAPYVLLELYYRGVAPIAMVNTEIDQQMAPACSLEGIPYAYSFDLD
                     VNNLMKDGDEVALERRGDHVYLRVLKTGRG"
     gene            complement(9374..9447)
                     /locus_tag="PLC06_04290"
     tRNA            complement(9374..9447)
                     /locus_tag="PLC06_04290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9411..9413),aa:Val,seq:cac)
     gene            9504..10163
                     /locus_tag="PLC06_04295"
     CDS             9504..10163
                     /locus_tag="PLC06_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89696.1"
                     /translation="MNDQAKNQTLIRAGLLLIFGMLIALAGCRSGDNNNLPFIETFDE
                     PGNWGVGEDAYSIGKVVNGVYDFKVLENDISRWASAGKTFGDGIYEVEATPVEGPLDN
                     GYGLLFRANPEKGDFYLFKISGDGYVWIGRYRDGAEEATIIGDHWFASPAITPGLNVT
                     NKLRVQAEAGNMIFYVNDQEVGRVTDNTFTTGDVGILVQTLGGPGVRVLFDNLSVKSL
                     P"
     gene            10429..12867
                     /locus_tag="PLC06_04300"
     CDS             10429..12867
                     /locus_tag="PLC06_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRO89697.1"
                     /translation="MLETQKTTDKKPITQQILMSTAEVLRDYTLALQSRQASVIGRRE
                     VLNGRAKFGIFGDGKEVAQIAMAKVFKPGDFRSGYYRDQTFMFAIGQTTIQQFFAQLY
                     AHADVAAEPNSAGRQMNAHFSTRLLNEDGSWRSQTETLNSSTDLSPTAAQMPRLVGLG
                     YASRLYRELNELHHLTRFSHNGNEIAFGTIGNASTAEGHFWEAVNAIGVLQAPVILSI
                     WDDGYGISVPNEHQITKGNLSELLSGFQRRPDSPEGFEIFTVKGWDYPALLETYARAA
                     ELARDKHIPCIIHVTEMTQPLGHSTSGSQERYKSPERLAWERDHDCLLRMREWIIDQR
                     IAPPGELDNLERNAEKLVENLRVKAWSAFSRPIYDERQQAAELFQKIEATSPRAEQIA
                     AIRRALLAKEAPLRRDILPAVQEVLVLTREEGREAGSPIARLIEWKREQEALDSERYG
                     SHMYSRSAESALNVVEVKPIYEDDAPQVPGSKILNAAFDAILARDPRVVAFGEDVGLL
                     GGVNQTWAGLQAKYGELRVADTGIREATIAGQAIGLAVRGLRPIAEIQYLDYLLYALE
                     ILSDDLASLHWRTRGGQKAPVIISTRGHRLEGIWHAGSPMGAIVNLLLGMHICVPRDM
                     TQAAGFYNTLMRGDEPGLVVEVLNGYRLRERLPSNIGEITVPLGVPEVLREGGDITLV
                     TYGAACRIAMEAAEALDKTGIDVEVIDVQTLLPFDRHGMILESLKKTNRIVFLDEDMP
                     GGATAYMLQNVIETQGGFQWLDSEPRTISAKPHRPAYGSDGAYFSKPNMEDVFATLYD
                     LMNEANPARYPSFQ"
     gene            12914..>13008
                     /locus_tag="PLC06_04305"
     CDS             12914..>13008
                     /locus_tag="PLC06_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89698.1"
                     /translation="MGEGVIEGTLARWLVKEGERIEQYAPIAEVET"
     assembly_gap    13009..13108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13109..13964
                     /locus_tag="PLC06_04310"
     CDS             <13109..13964
                     /locus_tag="PLC06_04310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRO89699.1"
                     /translation="GTGRDGRITKEDVLAYVESQKTAAVPASAPLVESRQETPPATPI
                     PAKPETGHTPSVSHDGSADLLPLTSIRRSIAEHMVRSVQTSPHVTTVFEIDFSAVAAH
                     RRANKDQFTRDDVRLTFTAYIVAATAQALKQHPLVNSSWSDAGILLKREINIGMATAI
                     DDGLIVPVIKHADGMNLLGLARTINDLADRARNKQLKPDDVRGGTFSITNHGTTGSLF
                     ATPIINQPQCGILGVGAIEKRVKVIDDAIAIRPMAYISFTFDHRILDGASADHFVSTI
                     KSLIENWR"
     gene            complement(14042..14410)
                     /locus_tag="PLC06_04315"
     CDS             complement(14042..14410)
                     /locus_tag="PLC06_04315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRO89700.1"
                     /translation="MRTYDIDFANIVHNIVYLRWLEDLRSEILVDVLPIDEILATGIS
                     PILTRTEIDYRWPVRIGDAVVGRMWASGLSRTRWTLSSEIVVGDQVCAAGRQSGYFAD
                     LKTLRPVRMPEKLKVLWNEG"
BASE COUNT         3188 a         4272 c         3990 g         2815 t
ORIGIN      
        1 aaacgattcg accattccga cctgatactc aaccgatggc ctgggcgtcg cctgagtcgg
       61 gttcgcgccc aaataggcgg ccgcgaggcg gacatcatca cgcgtcgggg ggttatcggc
      121 aatcaattca gccactacgc tctcaagggt cgtgggcttg gaaagcggca acggcgaagg
      181 ctccgctgca ggtgacgcaa tcgcctcggt tggggatgcc ggcaactcat cgagagttgg
      241 cgtcatcgaa ggagtgatta tgctcggttc cggggttgcc gtttgagcaa cctgaacggt
      301 cgccgttgat gctatttcag cttgcgccgg gatcgattcc gggcgaacac aagccacgag
      361 acaaagagac aagagcaata acgaaaaggt aaatcgcatg ggacggaatt ctacaacgag
      421 ccgggatcta tcgatagcaa tcaagttacg attcaggcaa ttcttaccat ccgggtacaa
      481 ttagcctcat gctatcgctg gatgcacaaa acaaatggcg ggatgaatat cgaaagaggc
      541 atcccggatg gcgacccgca accgaagttt tcgcggagca agttcacgag aacctacggc
      601 cccattcacg tctgctcgac gtgggctgcg ggcgtggcgg cctcgtcgaa caactggagc
      661 atccgctctc gcaggcggtc ggcatcgacc ccgacttctt ctcgctggcg gagcatcgtc
      721 tggaaaacct ccctcgcgcc gttgcgctca gcgaccatct ccccttcgtc aatgacgtct
      781 ttgatatcgt cattgccagt tggctattgg agcacctgcc caatccagtg agcacattcc
      841 aggcgatcgg ccgcgtgctt aggccgggcg gggtgtttat tttcatcacc cccaacaagc
      901 gacaccccct gaccgtcgcc aaccgcttcg tcggaaggct gggcagcctt caggggcggc
      961 tggttgcccg actttacggc cgcgcgcccg aagacacctt ctccacattc tatcgcgcta
     1021 acacggccac ggagctgcac gacctgacca cctcggccgg gctagtcacg gaatccatca
     1081 caatgattgc cgatccttcg tatgttgcct tcaatcgcct gttgttccat gggatgagcg
     1141 cgattgacaa ggggctgcca caagatcggc aaatccatat cgtcggagta gcccgaaaga
     1201 tataattgtg gataatcgcg acatttcggc ggcatgttag catcattttc tgagaggtcg
     1261 atcaccaata tacgtcacag aaatggacgg attcgcttcc ggataatccg cgtagcttgt
     1321 gaaacctgcg gacgctcatt ctaggtagat tcacgagatg gaatccttat cacctctgcg
     1381 ccgcgcctac tataactatc taagccgtgc ctacgcgatg atgtatcgat tgcacaaacg
     1441 atccttgttg ggaattcgcc tcgtttcgat cgcgcgatgg ttgcccatcc tggtgttgat
     1501 aatcggctgg cgacaacgtt ggcccctgcc ggcgctcatt ggnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnccggcaca cgaaaacggc gacgattccg gtttgccgcc
     1681 tcccaagcgc gtcaccatct atctgagtca tgataatccg gcgacacgcc aggcaatcct
     1741 ggacactctc atcagcgacg cccgccaggc gcgcctcgat gccggatgac cctatgttcg
     1801 ctcaacgttc gcaaaataga tatagttgat agaccatgaa cgaatcagag gcgcttctcg
     1861 tcgtggaacg agggccagtc cccaccaccc agatccaact tcaaaacgat caattcaccg
     1921 tcggccgcag cgcgggcaac gatctcgttc tcgccgatcc cgaagtgtca cgacgacata
     1981 tccgcgtcgt tcgtcgcgcg gacggccatg ccgtcgaaga tatcggcagc accaacggca
     2041 cattcgtaaa cggccagcgg atcagtcatc tgaccctctt gcaagatggg gatgccattg
     2101 atttgggcga tacggtgcgt ctgcgttata tgtcattggc tccagcagaa gatgatgacg
     2161 ataccggccc cttgggtccc acggccgaga agccgacgca ggcttacaca ccccctgctg
     2221 cccccgccga agagcgcccc gtggagcgac cccaacccat cgcacctgta gcgcataagc
     2281 cggccatcgc acctgtagcg cataagccgg ccgttacgcc aacgctaccg ccacccacgc
     2341 cggcataccc accaccgcca acgtatgccc acgaggaacc ccggcgtggg cgcggcttgt
     2401 ggattggctg cggcctgttg gtcgcgctgc tcctggtctg cgcgggcaca ttcctcgtgc
     2461 tggacgccta tgaccagggc cggctgctat actgtggccc attggctccg atgttccaaa
     2521 tcatcctggg gccgtttggc tttgccccaa tttgctcgta atccagcccc tgctccagat
     2581 ctatgcccgt atcggaaaaa ccccaactgc gcgtactcga cttccagccg gtcttccatc
     2641 gcggggagcg aatgtggctc ctgcgcgatc catggcaatt aagcgatcgc cagctcatcg
     2701 tgccccaggc aatcgctcag atgctcctgc tctgtgacgg gacgcggact gccgccgagc
     2761 tccgcgcgga gatagctgag gaactgggca tcgatattcc atttgaagta gtcagggacg
     2821 cgctctcgca actggatcaa gtctatttgc tccataacga acgattcgag gcccaacatg
     2881 cccatgccct ggctgaatat cgcgctcacc cctatcggcc gccggctctg gcgggtttag
     2941 gttatccggc ttcacccgca gagttgatca cgcaatttga aagttacggg aatgctactc
     3001 agggccgcga cccggtctcc caagcgtggc gcggcgtggt atcaccccac atcgactacc
     3061 agcgtggcgg gccggtctac gcccaagtct ggcaggaagc caaaagcgcc gtgctggatg
     3121 ccgagctcgt gctcattttc ggcaccgacc acaatggcag cgccggatcc atcactctga
     3181 cccatcagcc ctatgccaca cccttcggcg tgttgccgaa tgatatggat ctcatcaaca
     3241 agctcgcggc agcgattggt gaggaaagcg cctatgccga ggaactcaac catcgcaacg
     3301 aacattccat cgagctttcg gccgtatggc tgcactatat ctttcatcag gcaaagcgcg
     3361 cgccatgccc aatgatcccc atcttgatcg gatcattcca ccacttcttg agcaatggcc
     3421 accacccggc caccgacccg acattccacc gcttttggga tacattgaaa cagggaactg
     3481 ccggccgtcg tgttttggca gttggctcag tggacctcgc ccacgtcggt ccggcatttg
     3541 gagattcatt tatcatggat gctcctcgtc gtatgtcact acgcgaagag gatcgcgggc
     3601 taacgtcagc catcacgcga ggaagtgccg ccgaattcta cgatcggatt ttcgcggtac
     3661 atgaccggaa ccgaatttgc ggattcgccc ccctctatca catgctgagc tatctcaacg
     3721 cgacaacagg cacccggatt gcctacgacc aatgcccggc cgatcagatg gatacctcac
     3781 tcgtgtccat ttgcgggctt ctgctgcaat agtccacctt ccttttcacg ccatcttctc
     3841 ggttcgccgc cacaatgtaa aaacattctt tatagagaat ggctctattg acttgggtaa
     3901 ataactgttg tattgttgca atatgttgta atatcccgca tgacatgaac aacattttgt
     3961 tgagcggttt ccggggaagt gaatcgcctc aagtcagcgg tggtaattat gaacaaagag
     4021 acgttactgg aaagttattc tgatgtcgat caagttagca agcgactagg gatacatccc
     4081 gagtcggtgc ggcggcttat ccgcgatgga aagctgcccg cgatcaagtt cgggaacaag
     4141 tggctggtgg aaaaatcagt cctggaacag tttgcaagcg gctacgatgg ccgtccaggc
     4201 aacaaggcaa cgctgttcta gttcccagat ttgctcgaca tcccggctct ctttgttcgc
     4261 tacggaagga aaagggataa gaggatgcaa caaacaacga atacggattg ctccgccagc
     4321 atccgcgttg tcggtgttgg aggagccggc agcaatgcgg taaatcgcat gattgccgaa
     4381 ggtgtcaaag acgtcgattt cgtcgtcatc aacaccgata atcaggctct ctccctttcc
     4441 gatgcaccgg tgcgcgtgcg aatcggcgac aagctgacca gagggctggg tgccggcggc
     4501 tttcccgatc agggagaaaa ggccgcgaat gagtcgatcg aagagattca gtcggtcctt
     4561 caaggttccg atatggtctt tgtcaccgcc ggcatgggcg gcggcacggg cacgggggca
     4621 gctccagttg tggcccgcgt agctcgcgag atgggcgcat tgaccatcgg cgtcgtcact
     4681 cggccatttc attttgaggg cagccggcgc gccacatcgg ccgagcgcgg aatcgagcag
     4741 cttcaaaagc acgtcgatac gctcatcatc atccccaatg accgcctgtt ggacatcacc
     4801 gacaaacgga tgccgcttca cgagtcattc catctggccg acgatgtcct gcgccaggga
     4861 atccagggga tcacagagct gatcaccgtg ccggggctga tcaacctcga cttcgccgac
     4921 gtgaagacga tcatgaccca gggtggcgca gcattgatgg ccgtgggccg gggcaccggc
     4981 gaagacaggg cgcggatcgc cgccgagcaa gccgtcagtt cgcggctgct cgatgtcacg
     5041 attgacggcg cgcaaggggt attattcaac atcaccggag gcaccacgct cagcctgtat
     5101 gaagtcaatc aggcggccga ggtgatccgt gaaacgaccc accccgatgc caacgtcatc
     5161 tttggcgctg tcatcgacga gactatgggc gatgagattc gcatcacggt catcgccacc
     5221 cgctttgatc aggttcatat gcggcggcaa atgctccagc gtaatgatac cggcctgttc
     5281 gaaacggcag cccgtcaggc ccaatcaaag gaacgcgcgg aaagaaaacc ggaaccggcc
     5341 caggaacagg tagagcagga agctacgccc cgtttcgcgc ccggcaacct ggaagtgccc
     5401 gcctttctgc gacgaaaata acgattaccg gccatccggc cattcgcgcc ggatgataac
     5461 aacgcttgct cgcaggctac atcccctctc tgcagctccc cctgtttcgc aggacagggg
     5521 gagcaccttt tttaatcggt gctctcaagt aacgtaaatt gcgagccgcc cgatacccag
     5581 cactcatcac cctcagcatc ttcagggcat tggatacgcc accagccact ttggggatcc
     5641 cggccgatta cggtcacccg acgctccctc agcagaaagc ccactcgatc atagctcaca
     5701 ccagggccgg aacggatatt tagatccacc aggatgcgca agctgccctg cacatcttcc
     5761 atcggcgtat ccgccggaag cgtcgcggtt ggcgcaaaat tgggttcggt cgaggattcg
     5821 ccaacgctgc tggttgcagt agcaatggga ggtgggggta atgtgttggc ccttccggca
     5881 aatgcattgc aggccagcga cgagaaaagc aaggccagca gacccagtcc gatatgtacc
     5941 cggtcaccca ttccgttcct catatgccca atgatacctc tgataacaga taattacaga
     6001 ctatccattc tcatgcgtaa tggtagtcaa tcgaccctgt cggcctgctc ggttataatg
     6061 caatcatagt caaggacaaa catgttatga gttttcgcgg attcatcgaa acggccgccg
     6121 acaacggcga tctggtgaca atcgacaagg ccgtcgatac aacctttgaa gtggccaatg
     6181 tcgcccatgc attggaaggc cggccggtac tgtttaccaa cattcagaat tatcccggct
     6241 ggaggatttg ttccggcgta tgtgccgatc gcaagttctt cagcatggct ttaggcgtag
     6301 aggttcccga tctgcttcat cacctgaagg attcccttga gaatccggtg ccgccgccgg
     6361 ttgtagggtc aggttcgtgc caggaggtaa tcgtcgagaa cgtaaacctc aacgacctgc
     6421 cgatcctctt gcacctgcct caggacgggg gccattatat cgccagcaat gtggtcatcg
     6481 tcaacgatcc cgaactgggg cgcaacatgt gctatcaccg cctattgaga ttggacgaac
     6541 ggcgattcgc ggcgcgcatc gtcgaaaacc ggggcacgtt caacgccatg cagaaaactg
     6601 aaggcgacct gccggtggcg atctgcatcg gcgtgtcgct tggcgtccat ctggccgcgt
     6661 cgatgtcccc cccgccgggc gtcgacgagc tggccgtcgc caatgccttg gcccccaccc
     6721 cgctggtgaa atgcctcacg aatgatctgt acgtccccgc cgacgccgag atagtcctcg
     6781 aagggcgaat taccaaaggg ctgtatagcg aagggccgtt catggatctg acggagacca
     6841 tggacatcac gcggatgcag ccgatcatcg agatcgatct gataacccac cgtcaggacc
     6901 cgatcttcca tgccctgctt cccggcgggc tggagcacaa gctgctgatg ggcatgccca
     6961 aggaaccgac gatttttgcg gccgtgaatc aggtcacccg ctgcacgggc gcggtcatca
     7021 cccccggcgg cgcgtcctgg ctccacgcgg tcgtccaaat cgacaaacaa ggaccggacg
     7081 acggcaagct ggccatagaa gcagcattta agggacacgg ctcccttaag cacgtgtggg
     7141 tcgtggacac cgatatcgat ctctatgatc cggcccaggt agaatgggcg ttggcgacgc
     7201 gattccaggc cgatcgggat ctctacgtct acgccaatca acccggcagc tctctcgatc
     7261 cctccggcac ccatgtgccc ggccggaaga gcctgacggc caaaatggga ctggattgca
     7321 caatcccctg gggcgccgac cgaagcatgt acgagcgcgg agaatatggc gtcgtcgatc
     7381 tttccgagta tctttagaac cattgccggg gaggttgcct gacaaaacag acgacttcct
     7441 ctattttagg cggggcaatt tgcccatact taaggcacat ttatgaccat aactccttcc
     7501 atgttaaacg atgaacaacg cgcgatgctc gccggcgagc agggccgggc gcgccaaatg
     7561 ggtatgcgac tcctgctcga tatggccgcg atggccggcg ccgagcacct gtcgccaatc
     7621 ggcagcgccc atctctccgg cgtctcgccg ctaaccggcg ggcttggcct gcgccaattt
     7681 ctggcccgac tggccgaaga cccgggcgag ggtgtggcca tccctaccac gctcaacgcc
     7741 gcaggctgcg acgaagacca gttcgaagcg atgcgaatcg tcgtgcccgg atttcttgac
     7801 caccatcgag agatcatcaa gtcatatgca ggcctgggcg tccaaaccat tcaatcttgc
     7861 gtgccctatg aatgggatga ggttcagtcg aggcttaccc aggatatgcc cgccgcctgg
     7921 gcggagtcca acgcgatctg tttcgccaac tcctatagcg gcctgcgcac caatcgggag
     7981 tcaggtctat ccgcgctggc ctgcgccctg acgggatatg cgccaaacta cggtttgctg
     8041 gaagacgagg gacggcggcc aaatttggca gtcactgtga ccgtcgacct ggaagacccc
     8101 accgacttct cggtattagg cgattggatc ggcaaacaac gacggccaaa ttggcgcatg
     8161 ccctttgggc ctattccggc gatcatcggg ctgccggcaa ccctgaccca cgaacagcgc
     8221 aaggcgatga gcgcggccgc ggccaattat ggctcccctc tactccatat tatagaagga
     8281 gatgaattcc ccgaagccga gtatcaggat cgattgacct ttggccggac cgaactggag
     8341 cagcgttacg ccgagttaaa gccgaagaaa cccgtttctc tggtcgtcct gggctgccca
     8401 caagcctcta tcggcgagct aaaggcgatc gccggctatc tgcggggccg ccgggtgacg
     8461 gccgatccct ccggcccggg agaaccgccg cccctgtgga tattcacctc ggctatcacc
     8521 aaggccgttg ccgaacgcac cggcttggcg gacatcttcc ggaagagcgg cgcattgttc
     8581 ctggaaaaca catgccccga agtcgtcccg tatgatccag gctgggtcaa ccatgtcctc
     8641 acaaactcaa tgaaagccga gcattacatt aagtccggcc tgaacggcat ccccacctcg
     8701 gtcatgcgcc tggctgattg cgtcgccatt gccacaggcg acatggtgct atcctatgaa
     8761 gacgcgcatc ggatgacaac tactgcgtcg ggcaaaaaga acaagacgca agagacaaag
     8821 agttcgggcg caacggcgga gcaatcggca caggctgcct cagcagttgc cgttggtcag
     8881 gggttgcctt cccaaggcga tttcaccgtc atcggcgagg catttgtaac ggacacaccg
     8941 atcacattcc tcggctatgt caatcgcgag acaggggtca ttgaagaaga agatcatcca
     9001 gccaacgggc agagcatggc cgggaaaatc gccatcttcc cgcgtggcac cggctccagc
     9061 gtggcgccct acgtgttgct cgaactctat taccgcggag tagcccctat cgccatggtt
     9121 aacacagaaa tcgaccagca gatggccccc gcctgctcac tggaaggaat accctacgcc
     9181 tacagctttg atctggatgt caacaacctg atgaaggatg gggacgaagt ggctctggaa
     9241 cggcgcggcg atcacgtcta tctgcgcgtg ctaaagacag gcagagggta atgcctgaag
     9301 ttatcggaga ttatcgcgtc gtgggcctta aggaaagggc cttgggaaag ggtcttaaga
     9361 aaggggcaga aggtgggcgc gaagggactt gaacccctga cctcacggat gtgaaccgtg
     9421 cgctctaacc aactgagcta cgcgcccaag catcgagtag tatatcatat acctgtcgtt
     9481 agccaagaga taattgagtc aagatgaacg atcaagccaa gaaccaaacc cttattcgcg
     9541 ccgggctgtt gctcattttc ggaatgctca tcgcgctggc gggctgccgg agcggcgaca
     9601 acaacaacct gccctttatc gagacgttcg acgagccggg gaattggggc gttggtgaag
     9661 acgcttactc catcgggaaa gtcgtcaacg gcgtctatga tttcaaggtt ctggaaaatg
     9721 atataagccg ttgggcctcg gcgggcaaaa cgtttggcga cggcatttac gaagtagaag
     9781 ccactcccgt agaaggcccg ttggataacg gctacggcct cctgttccgc gccaaccctg
     9841 agaaaggcga cttctatctg ttcaagatat cgggtgatgg ctatgtctgg atcggccgct
     9901 atcgggacgg ggccgaggaa gccacgatta tcggtgatca ctggttcgct tccccggcaa
     9961 tcacgcccgg cctgaacgtg accaacaagc ttcgcgtcca ggctgaagcg ggtaatatga
    10021 tattctacgt caacgatcag gaagtcggcc gagtaaccga caacaccttc accaccggcg
    10081 atgtgggcat cctcgtccag acgctcggcg gcccgggtgt aagagtgctg ttcgataact
    10141 tgagtgtaaa gtcgctgcct tgagaaaata aaagttaacc ggcttgcatt tccgaccgcg
    10201 atttttgtgc tatgatgtaa ttatgatccg caaatgatac ggatctcgca ggagcggtaa
    10261 tcccactatc gcccgcaacc ttcgtgaagg tttggctgag ccgttaagcc gcctttcccc
    10321 gattggggaa tcaacagaat ggagaggatg tattgggttt ccctgccggt tcactttgta
    10381 tccgattacc catgttgaga gcggccgaaa attactctgg agcaagacat gcttgaaact
    10441 caaaaaacaa cagacaaaaa accgataacc caacagatat taatgagcac cgccgaggtt
    10501 ctgcgcgatt acacgctggc attgcaaagc agacaggcca gcgtgatcgg gcggcgtgag
    10561 gtgctcaatg gccgggccaa gttcggcatt ttcggcgacg gtaaagaggt agcccagatc
    10621 gcgatggcca aggtcttcaa gccgggcgac ttccgctcgg gatattaccg cgatcagacg
    10681 ttcatgttcg ccattggcca gacgactatc cagcaattct tcgcccaact ctacgcccat
    10741 gccgacgtcg ccgccgagcc taactcggcc ggccggcaga tgaacgccca tttctccacc
    10801 cgtctgctga acgaggacgg atcgtggcgt tcgcaaacgg agacattgaa ctcctcgaca
    10861 gacttatccc ccacggccgc ccagatgccc cgccttgttg gtctgggtta tgcgtcccgc
    10921 ctctatcgcg agctgaacga gctgcaccac ctgacccggt tctcgcataa cggtaacgag
    10981 atcgcgttcg ggacaatcgg caacgcttcc acagccgaag gtcatttctg ggaagccgtg
    11041 aatgccattg gcgtccttca ggcaccggtc atcctctcaa tctgggatga cgggtacggg
    11101 atctccgtgc caaacgagca ccagatcacc aaaggcaacc tgtccgaatt gctgtccggc
    11161 ttccagcgca gaccggattc acccgaaggc ttcgagatat ttaccgtgaa ggggtgggac
    11221 tatccggccc tgctcgaaac ctacgctcgc gcggccgaac tggcgcgcga caaacacatt
    11281 ccctgcatca tccacgtcac ggagatgacc caaccgctcg gccactcaac ctccggcagc
    11341 caggagcgat acaagtcacc tgaacgactg gcctgggagc gcgatcatga ttgtttgctc
    11401 aggatgcgcg aatggatcat cgaccaacgc attgcgccgc cgggggaact cgataatctg
    11461 gagcgcaacg ccgaaaagct ggtcgagaac ctgcgcgtga aggcgtggag cgctttctcc
    11521 cgcccaattt atgacgaacg tcagcaggcg gccgaactat ttcagaagat tgaagcgact
    11581 tcaccccggg cggaacaaat cgcggctatc cgccgcgcct tgctcgccaa ggaagcgccc
    11641 ttacgaaggg atattctgcc ggccgtgcag gaagttctgg tattgacgcg tgaagaaggg
    11701 cgcgaagccg gctcgcctat tgccagactt atagaatgga aaagggagca ggaagccctc
    11761 gatagcgagc gctacggctc gcatatgtac agccgctcgg ccgagtcggc gctcaacgtc
    11821 gtcgaagtga agcccattta cgaagatgac gcgccccagg tgcccggatc gaagatcctc
    11881 aatgccgcat ttgacgccat actggcgagg gatcctcgcg tcgtggcctt cggcgaagac
    11941 gtgggcctct tgggtggcgt caatcaaacc tgggcgggcc ttcaggcgaa gtatggcgag
    12001 cttagggtcg ccgatacggg cattcgcgag gccaccatcg cggggcaagc catcggcctc
    12061 gccgtgcgtg gcttgcggcc gattgccgaa attcaatatc tcgactatct gctctacgcg
    12121 ctggaaatcc tgtccgacga tctggcctcg ctccattggc gaacgcgcgg tggtcagaag
    12181 gccccggtca tcatcagcac gcgggggcat cgtctggaag gtatctggca tgccggatcg
    12241 cccatggggg ctatcgtcaa tctgctgctg gggatgcaca tctgcgtccc gcgcgacatg
    12301 acccaggcgg ccgggttcta caacacgctg atgcgcggcg atgagccggg gctggtcgtc
    12361 gaagtgctga acgggtatcg tttgcgcgag cgcctgccgt caaatatcgg cgagattacc
    12421 gtgccactgg gtgtgccgga ggtgctgcgc gaaggcggag atatcacgct ggtgacctat
    12481 ggcgcggcat gtcgaatcgc catggaggcg gccgaagcac tggataagac aggcatcgat
    12541 gtcgaagtga tcgatgtgca aaccctgtta ccctttgatc ggcacggcat gatcctggag
    12601 tctctcaaga aaacaaaccg gatcgttttt ctggacgaag acatgcccgg tggagcgacc
    12661 gcctacatgc ttcagaatgt gatcgaaacc cagggtggct tccaatggct cgattccgag
    12721 ccgcgcacca tctctgccaa gccccaccgg cccgcctatg gatctgacgg cgcatacttc
    12781 tccaagccca acatggaaga tgttttcgct acgctgtatg acttgatgaa tgaggccaac
    12841 ccggcccgct atccgagctt tcaataataa cggagaaaac acttatatgg catccaggat
    12901 aacgctgccg gaaatgggag aaggggtcat cgaagggacg ttggcccgct ggctggtgaa
    12961 agaaggtgaa cgcatcgagc agtatgctcc aatcgccgag gtggagacnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcaccggccg cgacggccgc atcaccaagg
    13141 aagacgtgct ggcctatgta gaaagccaaa agacggcggc agttcccgca tccgcgccgc
    13201 tggttgagag caggcaagag accccaccgg caacgcccat tcctgccaaa ccggagacag
    13261 gccacacccc gtccgtttcg catgacggct cggcggattt gctacctctg accagcattc
    13321 gacgctcgat tgccgagcac atggtgcgga gcgtgcagac cagcccccac gtgacgaccg
    13381 ttttcgagat cgatttctcg gccgttgccg cccatcgcag ggccaacaag gaccaattta
    13441 cccgcgatga cgttcgtctg actttcaccg cctacatcgt cgctgccacc gcccaggcac
    13501 tgaaacagca ccctctggtg aacagcagct ggtccgacgc gggaatcctg ctcaagcgag
    13561 agatcaatat cggcatggct accgccatcg atgacgggtt gatcgtgccg gtgatcaagc
    13621 acgccgacgg catgaacctg ctggggctgg cgcgcacgat caacgacctg gccgatcggg
    13681 cgcgaaacaa gcaactaaag cccgacgacg tgcgcggcgg caccttctcc atcaccaacc
    13741 acggcacgac cggcagcctc ttcgctacac ccatcatcaa ccagccacag tgtggcatcc
    13801 tgggcgtcgg cgccattgaa aagcgagtca aggtgatcga tgacgctatc gccattcggc
    13861 caatggccta catcagcttc accttcgacc accgaatcct ggacggtgcg tcggccgatc
    13921 atttcgtcag cacaatcaag agcctgatcg agaactggcg ctgagaaaaa accgggaccc
    13981 cgacaacaaa aaacgcgagt cgccaaccac gactcgcgtt tttatatgga ttgaatcagg
    14041 gttatccctc attccataac actttcagct tctccggcat tcgtaccggc cgcaaggtct
    14101 tcaaatcggc aaaatagcca ctctgcctgc cggcggcgca cacctgatct cccaccacga
    14161 tctccgacga cagcgtccag cgcgtgcggg acagaccaga cgcccacatg cgaccgacaa
    14221 ctgcatcccc gatgcgcacc ggccaacgat aatcgatctc ggttcgggta aggatggggc
    14281 tgatgcccgt cgccagaatc tcgtcgatcg gcaacacatc aaccagaatc tcggaacgca
    14341 gatcttccag ccagcgtaga taaacgatat tatggacgat gttggcgaag tcaatatcgt
    14401 aagtgcgcac gcagcaattc gaaatatgac tcagtcgggt tcgagttcaa gctgaacgaa
    14461 cataa
//
LOCUS       DAOMQG010000045        14390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1816_length_14390_cov_10.812278, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000045 DAOMQG010000000
VERSION     DAOMQG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_14390_cov_10.812278"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLC06_04320"
     CDS             <1..360
                     /locus_tag="PLC06_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89701.1"
                     /translation="REDLWLMLLLKGTHTIIGGSGLHNIDWDVPKFEIGYWVRSSYAG
                     QGYITEAVNGITAFAFNSLGAQRLEIRCDVRNTRSAAVARRAGYTLESTFHSDARDHF
                     DRLRDTFIFVKVADREE"
     gene            complement(441..1313)
                     /locus_tag="PLC06_04325"
     CDS             complement(441..1313)
                     /locus_tag="PLC06_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRO89702.1"
                     /translation="MKIAVLSDIHGNLPALEAVVAHIDAWSPDLVVVDGDIINRGPLS
                     GDCLDLVVERHHSQGWYLLRGNHEEFVLDCGGPDQPLSGPVFEMKRFAHFALAQVVDH
                     VDLLRRLPDEFEQEGPDGQLLRIVHASMRSNRDGIYPMTPEEEIRRQIAPAPAVFVTG
                     HTHRPLMRQVGDTLVVNIGSVGSPFDKDRRAGYGQFTWHRDHWQAEIVRVDYDYGQIE
                     KDYAQSGFLEEGGPLAQIMLVELRKARGLIHHWAAQYEEAVISRSLTLEESVRLVLCE
                     DNIRPFTGPPGWIV"
     gene            1288..2190
                     /locus_tag="PLC06_04330"
     CDS             1288..2190
                     /locus_tag="PLC06_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRO89703.1"
                     /translation="MSESTAIFISAIIANAGQGSRKEGSYSILSSEFDMLAAVFVFLS
                     KFLPLFVFPLGFIALLLIVAALWQRGRRALVLLALAVLWLAGSRYVAYTLIRDLETRY
                     PPLAPGQQADAIVVLGGGTRGSDPPRPMVEVNEAGDRLLYGATLYHAAAAPIVLVTGG
                     SIEWLTPEGVGPEANDMSSLMGLLGVPPEALWLESRSRNTYENALFSREMLAEAGMTD
                     ILLVTSAMHMPRSVPLFEAQGLNVTPAPTDFLVSDAEWHHLWRGGPTSTIINLLPNVE
                     YLTYTTRALKEYIGLIVYGFRGWL"
     gene            complement(2193..3677)
                     /locus_tag="PLC06_04335"
     CDS             complement(2193..3677)
                     /locus_tag="PLC06_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HRO89704.1"
                     /translation="MTEQQSQEQEGKDGKQATASPPTDTISQTSHSVTIGGKTLHYTA
                     TTGRIILKAEDEKEGEKPKAAIFFVAYTVNPPDEGDREAFENSRPITFAFNGGPGSSS
                     VWLHLGVLGPRRVVLEEDGRLLPPPYRLVDNEFSLLDRTDLVFIDPVTTGFSRAVPGE
                     KDKQFHGFKADIESVGDFIRLFISRYHRWGSPRFLIGESYGTTRAAGLSGYLQERHGL
                     YLNGIMLVSVVLNFMTLAFDPGNDLPYILFLPTYTATAWYHKRIAGNNLEELLAEVEA
                     FALGEYAAALMQGDALPANQKQKIAAKLAQYTGLSTSYIESTNLRINIFRFVKELLRD
                     QRRTVGRLDSRFTGIDRDAAGERNEYDPSYAVIQGAYSGTLNDYIRRELKFESDLPYE
                     TLSGRVHPWSFEQNQNSYVNVGETLRKAMSMNPHLRVFVANGYYDLATPYLATQYTFN
                     HLELDESLRDHVLMKHYEAGHMMYIHDPSLAQLRRDLDGFLDQF"
     gene            3824..>4938
                     /locus_tag="PLC06_04340"
     CDS             3824..>4938
                     /locus_tag="PLC06_04340"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRO89705.1"
                     /translation="MSSAITKTQLGNGLTVLLKEMHHAPVATFMVWYRVGSRNEIPGR
                     TGISHWVEHMMFKGTPTFPGAAMERMISREGGFWNAFTWLDYTAYYETMPSDRIDLAL
                     QLEADRMINTMMSPEEVESERTVILSERSMYENQPNFLLDEELTAAAFRVHPYHHEVI
                     GDAVDLRSMTREDLIAHYRRHYSPTNAIAVVVGDFNMAEMLSRIDELYGAIPGGEATA
                     PVVRDEPPQAGERRITVNGPGDTAYLTYAYRVPGAAHADYPALTLLNAAFAGGSSLGM
                     FAGGGSNRSSRLYKALVSTELAAGAYGGLTPTIDPFLYTIRAVVRAGRTIPEVEEALE
                     AELARLIDEPITEAELAKALKRARAQFIMAGESISGQA"
     assembly_gap    4939..5038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5039..6709
                     /locus_tag="PLC06_04345"
     CDS             <5039..6709
                     /locus_tag="PLC06_04345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRO89706.1"
                     /translation="EGANDRWGGRGFRLAAHYNLPSDLAVTNPDAWDKSCDCQTLCLQ
                     GKMTGAYNEIRCSRLRSVWGEGNELAVHASAPLQSGNRILGILNVAAPDWSWFTPRNL
                     SLLTNVGNQMGIALERARLFDALQEQRIHEQAALLDLSHQLLSRRNLDDLLRFIVEEV
                     RSQLQADACSVLLTGPDPDYLYFRAASGWLYNPVKEGRRAPANEETGSGRVMRTQRVL
                     QIEDLRQSEPRLWSSEWLDREGFRAAVMAPLVADGSSIGALVIDSRQPRVFTEGEIRF
                     LRLMANQVALALEKARLHKEELRHERVEEELTVARQIQMSMLPVTPPRLPGWQYATYF
                     EAAHQVGGDFYDYFPLPGEPGRWGIAIADVSDKGVPAAIFMAMSRTTIRNIALRGRSP
                     AEVLSWANRYIQEDSQSDMFLSAFYAELNMNTGQLIYANAGHNPPLLWRAGEKEFYRL
                     PATSPLLGVLANLDVSAASIDIYPGDVLILYTDGISEALDADYGEFGLVRLKDTIRNF
                     IERTLNPRAEDIKTAIADAVGAFTGNVMQHDDITLLIVKRDEVPDGSQ"
     gene            6696..7973
                     /locus_tag="PLC06_04350"
     CDS             6696..7973
                     /locus_tag="PLC06_04350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRO89707.1"
                     /translation="MAANSIPGPDSIHRHVLDNGITVLVYENMDSGTVSIEGLVWAGA
                     VNENRGNAGLANFTADLLMRGTHKRPFDEIFEELESAGAGVSFGGARHTSGFSGHSLV
                     EDFDLILDVIDESLRRPTFPAEQIERLRGQIVTGLQMRANNTRQMAWLSYYETAYQGH
                     PYSRAVGGYLDSIGNMNRADIVDFYDRYYGPRNMIIGVVGAISADDVLRKLEPVFGEW
                     RNDHQQITPLSPDIERPIGLQRRYVPMPEKHQADIVLGLPGPRRSAPDYLDASVMNTI
                     LGVFGMMGRIGQNVREEQGLAYYASSRLEGGLGPGSWNASAGVAPDKVEQAIESIRHE
                     IRRMIDEPVTAEELADSLAYRTGSLPMSLETNSGLVDIITDMELYGLGLDYLHRYKDE
                     INAITVERIQNAAQKYLSADDLVIAVSGPVEAL"
     gene            7973..8410
                     /gene="acpS"
                     /locus_tag="PLC06_04355"
     CDS             7973..8410
                     /gene="acpS"
                     /locus_tag="PLC06_04355"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRO89708.1"
                     /translation="MLAVGVDIFEVERMAQGMERHGARFCDRFFTEREQAQCEGRPTS
                     LAGRFAVKEAVGKALGTGIGDVAWKEIEIINDERGRPQLTLHGAAARLADELGLTEWA
                     ISLSHTRTHAVGMAVALMPERRMIDTISLETRADTRAPNKTGL"
     gene            8411..>8847
                     /locus_tag="PLC06_04360"
     CDS             8411..>8847
                     /locus_tag="PLC06_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRO89709.1"
                     /translation="MDDMTTAPAQRRLLVVLAHPDDESFGMGGTLARYADEGVDVHIA
                     IATDGVAGSVAKGHEDKLKELAKVRRNELEAAVRVLGGKLHMLGYRDSGYIGDPANHH
                     PEAFINSDFEEATGRVVALIRQIRPQVVVTHDETGGYYHPDHI"
     assembly_gap    8848..8947
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8948..9166
                     /locus_tag="PLC06_04365"
     CDS             <8948..9166
                     /locus_tag="PLC06_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcNAc-PI de-N-acetylase"
                     /protein_id="HRO89710.1"
                     /translation="LHARIDYRRYWDVKRAAGAEHPSQGGGPSRSRMLPESFQRRYMA
                     TETFIRAYPPVPDGFRENDLFAGVDATV"
     gene            9202..10149
                     /locus_tag="PLC06_04370"
     CDS             9202..10149
                     /locus_tag="PLC06_04370"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO89711.1"
                     /translation="MTFQRVMKILGILTGLAAAAAALVVAYFTRRMVAPARQPLWANP
                     GDMGLEYENAQFPALDSVRLSGWFIPATKSEDRHGAAIILVHDWTWNRLGDAAGDMLA
                     NLSGTTPVELLRLAYTLHYEGYHVLMYDQRNHGESAAHPPVTFGQSEANDLLGAIAYL
                     QTRSEVDGSRIGAIGFSMGANAILYAMAQTDQLGAAIAVQPMTIGTYVKGYTGDLLGL
                     PGRALLPLVETAYSAVSGVSIGALQPGFAVAGAGQTPVLFVQCKEDHWGSPDDTARLR
                     AVTPEGEGPLYVDGTHRYEGYQYLIENPRVAVTFFERHI"
     gene            complement(10207..12189)
                     /locus_tag="PLC06_04375"
     CDS             complement(10207..12189)
                     /locus_tag="PLC06_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89712.1"
                     /translation="MMDRQSQFLGMETSKARSVRGTLVRLWHYFQRYGLILLLVGVLV
                     ISSTYMQVLIPDLTGQAVDCYLGPYSMAAAAGGGAALGSFADNAEQANAFSNCWYTTP
                     DITATAGETLAGFGRLILLIVGLYVGSAVLSGFMFYMMSWAGYHVLRDLQAQVFNHIH
                     QLSLGFFTKHEAGDVMSRFTNDSDTLQQVIGFGLVSVLQGILLIGWIIVVMFQRNAPF
                     ALISLVTLPLMVIITRWFSEQARKAFRRARQQIGNVNADLQESISAVREVQAFSREEE
                     NIQNFRESNAANRDANIRAQAYTGALGPALEGLSYVSLAVVAGIGGLLMLRGGGLMGE
                     TISVGLIITFIGYTQQFNRPVQQISTLWATIQSAIAGAERIFGLLDEPVEIKDKPNAI
                     VMPPITGHVVYQDVFAEYNPDEPILRGISIDAKPGQSVAIVGPTGAGKTTIINLLPRF
                     WDVESGSITIDGIDVRDVTQQSLRRQIGIVLQDSFLFSDTVMNNIRYGRPDATDEEVI
                     AAAQLAHADDFINRLQDGYQTVLGERGAGLSQGQRQLLSIARVALIDPRILILDEATS
                     SVDTRTEREIQKALEELLQGRTSFVIAHRLSTIRTADQVLVLKDGQIIEQGTHRSLLE
                     KRGFYFDLYMSQFRRKSTEEDLPDDLNELELMEPGD"
     gene            complement(12239..14155)
                     /locus_tag="PLC06_04380"
     CDS             complement(12239..14155)
                     /locus_tag="PLC06_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89713.1"
                     /translation="MQLSGSQQAGKQKAGGRANLQSLGRALNYLTKYPRLTAGATVAL
                     IIATAAQLVVPQLLQRIIDTIVTSATHQGILNLPANIQAIAAQKLGLDLVAAQAELDA
                     APRALITAALIIVLFAVARGLFAFGQNFMSQALSESIAFDLRNDIFTKIQRLSFSYHD
                     RNRTGQLMIRATDDVERLRVFIGQGLLMALQALILLVGTLAILTWTNIRLTLVVLPVL
                     PLALGIFMLFGIIAQPLFVEVQKRLGRVNTTLQENLAGLKVVKAFTAEEREEKRFHEN
                     IDSYLDQRIKVSRVFSFLFPFIFLIANLGQGAVLYFGGRQIIDGTLTLGEWQKFSLYL
                     VYVFIPMGQLGFIISLMAQAGASADRIFEILDAKNDVENKPGAIDLGEIDGRVEFKDV
                     SFRYFPGGEAVLSHIAFTAEPGQTVALLGATGSGKSTIINLIPRFYDVSEGQVLIDSH
                     DVRDVTLESLRQHIGIVLQETVLFSGSIRDNIAFGRPDASDDEIIAAAKAAAAHDFIS
                     GFPQGYDTPVGERGATLSGGQKQRVAIARALLMDPHILILDDSTSSVDLNTEYEIQKA
                     LDQLMKGRTSFVIAQRISTVLNADLILVLDKGSIVARGKHEELMEASPIYAEIYHSQL
                     AEDIAPASAQPDLN"
     gene            complement(14181..>14390)
                     /locus_tag="PLC06_04385"
     CDS             complement(14181..>14390)
                     /locus_tag="PLC06_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRO89714.1"
                     /translation="GYIERQLDSDDRRRFNIHLSDAGRNLAYEHEHQHFQLIADLFSA
                     LSPQEIETLSALLHKLSDQISTLKE"
BASE COUNT         2915 a         3857 c         4265 g         3153 t
ORIGIN      
        1 cgtgaggatt tgtggctgat gctgctgctg aaaggaaccc acaccattat cggcggaagc
       61 ggcctccata acatcgattg ggacgtgccg aagtttgaga ttggttactg ggtgcgttca
      121 agctatgccg gtcaggggta tattactgag gctgttaacg gcatcaccgc cttcgccttc
      181 aactcgctgg gtgcgcaacg actggagata cgctgcgatg tcaggaacac acgcagtgct
      241 gccgtggctc gacgtgccgg atatacgctg gaaagcacct ttcacagcga tgcgcgagat
      301 cattttgacc gactccggga tacatttatc ttcgtaaagg tggcagatcg ggaggaatag
      361 cagacgttct ctactgaagg actgatcaac tgaagaactg atcaacggtc aattcggcgg
      421 cagcacaaca gttcgcgcaa ttatacgatc catccgggcg ggccggtgaa tggtcgaatg
      481 ttatcttcac agagaacaag ccggacactt tcctcaaggg tcaggctcct gcttatcact
      541 gcctcctcgt actgggcggc ccaatgatgg atgaggccgc gcgctttgcg tagctcgacc
      601 agcatgatct gcgctaacgg ccctccttct tccaggaagc cggattgagc ataatccttc
      661 tcgatttgcc cgtaatcgta atccacgcgg acgatctctg cttgccaatg gtcccgatgc
      721 caggtgaatt gcccataacc tgcacggcga tccttgtcga aaggagagcc gaccgagccg
      781 atattgacga caagggtatc gccaacctgt cgcatgagcg ggcgatgggt atggccggtc
      841 acaaaaacgg ccggagccgg agcgatctgg cgtcgaatct cctcctcggg ggtcatggga
      901 tagatcccgt cgcgattgct tcgcatggat gcatggacga tccgcaatag ttggccatct
      961 ggtccctcct gctcgaattc gtccggcagc cggcgcagca gatcgacgtg atcgacgacc
     1021 tgggccagcg caaaatgggc gaatcgcttc atctcgaaca ccgggccaga caggggttgg
     1081 tcaggaccgc cgcaatcgag cacgaactcc tcgtgattgc cgcgcagcag ataccacccc
     1141 tggctatgat gtcgctccac caccagatcg aggcaatccc ccgacagagg ccctcggttg
     1201 atgatgtcgc cgtccacgac gacgagatcg ggcgaccagg cgtcaatgtg ggcgaccact
     1261 gcctccagcg cgggcaggtt gccatgtatg tcggagagta ccgcgatctt catctccgcg
     1321 attatagcga atgccgggca agggagcaga aaggaaggca gctattcgat cttgtcgtcg
     1381 gaatttgata tgctggccgc cgtgtttgtc tttctatcga aattcctgcc actgttcgtt
     1441 ttccctttgg ggttcattgc cctcttattg atcgtcgccg ctctctggca gcgcggccgt
     1501 cgagcgctgg tactactcgc tctcgctgta ttatggctgg ccggcagtcg ttatgtggcc
     1561 tatacgttga tccgcgatct tgagacgcgt tacccaccgt tggcgccggg gcaacaagct
     1621 gatgccatcg tcgtcctggg aggtggcacg cgtggatcgg atccaccgcg gccgatggtg
     1681 gaagtgaacg aggccggcga tcgccttctt tatggggcca cgttgtatca tgccgccgcc
     1741 gcccccatcg tgctggtgac aggcggcagc attgagtggc ttacccccga aggggttggc
     1801 cccgaagcga acgatatgtc gtcgctgatg ggcctgcttg gcgtgccgcc tgaagcgctg
     1861 tggctggaga gtcgctcgcg caatacctat gagaacgcgc tcttcagtcg ggagatgctg
     1921 gccgaggcag gcatgaccga catactcctg gtgacctcgg ccatgcacat gccccgttcc
     1981 gtccctcttt tcgaggccca agggctaaat gtcacgcccg caccgactga tttcctggtg
     2041 tcggacgctg aatggcatca tctctggcgt ggcgggccga cttcaacgat tatcaacctg
     2101 ttgccgaacg ttgaatatct cacctacacc acccgtgcgc tgaaggaata tatcggcctc
     2161 atcgtttatg gctttcgtgg ctggttgtaa gatcagaatt gatccagaaa cccgtccagg
     2221 tcgcgcctca actgagccag ggacgggtca tgaatgtaca tcatgtgccc tgcctcataa
     2281 tgcttcatga gcacgtggtc gcgcaggctt tcgtcgagtt ccaaatgatt gaaggtgtac
     2341 tgcgtcgcca gatagggcgt ggccaaatca taataaccgt tcgccacgaa aacgcgtaag
     2401 tgggggttca tgctcatcgc cttgcgcaac gtctcgccta cattgacata actgttttga
     2461 ttttgctcga agctccatgg atgaacgcgc ccgctgaggg tctcgtaggg cagatcgctt
     2521 tcgaatttta gctcacgccg gatgtagtcg ttcaatgtgc ccgagtaggc gccctggatc
     2581 actgcgtagc ttggatcata ttcatttctt tctccggcgg cgtcgcgatc gatgcctgtg
     2641 aaacggctgt cgagacggcc gaccgtgcgt cgctgatcac gcagcagctc tttcacgaag
     2701 cggaagatgt tgatgcgtaa attggtcgac tcaatatagc tcgtcgacag accggtgtat
     2761 tgggccagct ttgcggctat cttctgtttt tggttggcag gaagcgcgtc cccttgcatg
     2821 agcgccgcgg cgtattcgcc cagtgcgaat gcttcgacct ccgccagaag ctcctccagg
     2881 ttatttccag cgattcgctt gtgataccag gccgtggccg tatacgttgg aaggaaaaga
     2941 atatagggca gatcgttgcc cggatcgaat gcaagcgtca tgaagttgag gactacagag
     3001 acgagcatga tgccgttcag gtatagccca tgacgctcct gcagataacc ggacaggccg
     3061 gccgcgcggg tcgtgccgta gctttcgccg atgagaaagc gtggtgatcc ccagcgatgg
     3121 tagcgggaga taaacaggcg gatgaaatct ccaaccgatt caatgtcggc cttgaaccca
     3181 tgaaactgct tgtccttttc gccggggacg gcgcggctga agccggtagt cactggatcg
     3241 atgaatacca gatcggttct gtcgagcagg ctgaactcat tatcaaccag tcgatagggt
     3301 ggcggcaaca aacggccgtc ctcctccagc acaacgcgcc gtggccccag aacgccaaga
     3361 tgaagccaga ccgaagagga tcccggcccg ccattgaatg cgaaggtgat cgggcggcta
     3421 ttttcgaacg cttctctatc tccctcatcc ggcggattta ctgtataagc tacgaagaaa
     3481 atggccgcct tgggcttttc accttctttt tcatcttccg ccttgagtat gatgcggccg
     3541 gttgtcgccg tgtaatggag tgttttgccg ccaatcgtga ctgaatggct tgtctgcgaa
     3601 atcgtatccg ttggcggaga ggcagtcgct tgttttccgt cttttccttc ttgttcctgg
     3661 gattgctgtt cagtcatgat attctccaat tgagtcaggg tgtggcccca gattacccac
     3721 aatcggctaa atcgtcacct attgaggctg aacgcattgg ctgactgccc gcatgagcag
     3781 gggtataatc tcgctctgac tggattatcg caggagtgtg ttcatgagtt cggcaatcac
     3841 caagacgcaa ctggggaatg gtctgacagt tctcctgaaa gaaatgcacc acgcgccggt
     3901 ggcgacgttt atggtctggt atcgggtggg cagtcgcaac gagatccccg ggcgcaccgg
     3961 aatttcccat tgggtcgagc atatgatgtt caagggcaca cctacatttc ccggtgcggc
     4021 catggaaagg atgatctcgc gggaaggagg gttttggaac gccttcacct ggctcgacta
     4081 cacggcctat tacgagacga tgccttctga ccggattgat ctcgctctgc agcttgaggc
     4141 ggatcggatg atcaatacca tgatgagtcc ggaagaggtt gaatccgagc ggactgtcat
     4201 cctgtcggaa cggtcgatgt atgaaaacca gcccaatttt ctgctcgacg aggagctaac
     4261 ggccgcggcg tttcgcgttc acccctatca tcatgaggtg atcggcgacg ccgtggacct
     4321 gcgatcgatg acgcgagaag acctcatcgc ccattatcga cgtcattatt cgccgaccaa
     4381 cgccatcgcc gttgttgtgg gggatttcaa catggccgag atgctgtctc gcatcgatga
     4441 gctgtatggg gccattcccg gcggcgaggc gacggcgccc gtcgtgcgcg atgagccacc
     4501 ccaggcgggt gagcggcgaa taaccgtcaa tggccccggc gacacggcgt acctcaccta
     4561 cgcctatcgc gtgcccggag cggcacatgc cgattatcct gccctgacgc tgctcaatgc
     4621 cgcttttgcc ggggggagca gcctgggcat gttcgccggc ggcggatcga atcgcagttc
     4681 gcgcctgtac aaggcgttgg tgagcaccga actggccgcc ggggcctatg gtggcctgac
     4741 tccgacgatc gaccccttcc tctacacgat ccgcgcagtt gtccgtgccg ggcgcaccat
     4801 ccctgaggtg gaggaggcgc ttgaagcaga attggctcgc cttatagacg agccgatcac
     4861 cgaagcagaa ctggcgaagg cgctcaagag agccagggca cagttcatca tggccggcga
     4921 gagcatttcc gggcaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     5041 gggggcgaac gatcgctggg gtgggcgcgg ctttcggttg gctgcgcatt acaacctgcc
     5101 gtctgatctg gcggtgacca atcccgatgc atgggataaa agctgcgact gccagacgct
     5161 ctgtctgcag ggcaagatga ccggcgcgta caatgaaatt cgttgcagtc ggttgaggag
     5221 cgtatgggga gagggtaacg aattggcagt tcatgcatct gctccacttc agtccggcaa
     5281 ccgtattttg ggtatcctca acgttgctgc gccggattgg tcctggttca ccccgcgaaa
     5341 tttgagctta ttgacgaatg ttgggaatca gatgggcatc gctctggaac gggcacgact
     5401 attcgacgcg ctccaggagc agcgcatcca cgagcaggcg gccttgcttg acctgtctca
     5461 tcaactcctg agccggcgta atctggacga tctcctgaga tttatcgttg aggaggtgcg
     5521 cagccagttg caggctgatg catgctctgt gttactgacc gggcctgatc cggattatct
     5581 ctattttcgg gcggcttccg ggtggctcta taacccggtg aaggaaggcc ggcgggctcc
     5641 tgccaatgag gaaacaggat ccggccgggt gatgcggacg caacgggtgc tccagattga
     5701 ggatttaagg caatccgagc cgcggctttg gtcttcggaa tggctggatc gggaaggatt
     5761 tcgggctgcg gtcatggcgc ctctggtggc agatggaagc tcaatcggag ctttggtcat
     5821 tgattctcga caacccagag tatttacgga aggtgagatc cgatttctgc gtttgatggc
     5881 caatcaggtc gccctggcgc tggagaaagc gcgcttgcat aaggaagagt tgcggcatga
     5941 aagggtggag gaagagctga cggtcgctcg gcaaattcag atgagcatgt tgcctgtcac
     6001 cccgcctcgg cttcccggct ggcaatatgc aacctatttc gaggcagccc accaagtagg
     6061 cggcgatttt tatgattatt tccccttgcc gggcgagccg ggtcgatggg ggatcgccat
     6121 cgccgatgtg agtgataaag gcgtcccggc ggctatattc atggccatga gccgcacgac
     6181 tatccggaat atcgctctgc gcggccgttc tccggctgag gtgttgtcct gggcgaatcg
     6241 atatattcag gaagacagcc aatcggacat gttcctgtcg gcgttttatg ccgaactaaa
     6301 catgaacacc ggccaactga tctatgccaa tgccggccat aacccgcctc tattgtggcg
     6361 cgcgggagag aaggagtttt atcgtttgcc ggcaacctct cccttgttgg gcgtactggc
     6421 caacctggat gtttccgctg cgagtatcga tatatacccg ggcgatgtgc tgattctcta
     6481 caccgatggg atatccgagg ccctggatgc agactacggc gagttcggct tggtccgttt
     6541 gaaggataca atccgaaact tcattgaacg cacactcaat cccagggctg aagatattaa
     6601 aacagcaatt gccgatgcgg tgggtgcttt caccggaaat gtgatgcagc acgatgatat
     6661 taccttatta atcgttaagc gtgatgaggt gcccgatggc agccaatagt atccccggcc
     6721 ctgactctat tcaccggcac gtgcttgata atggcataac cgtgctggtc tatgaaaata
     6781 tggacagcgg cacggtgtcg attgaaggcc tggtttgggc cggcgctgtc aatgaaaacc
     6841 ggggaaatgc ggggctggcc aacttcacgg ccgacctgct catgcgcggc acgcataaac
     6901 gcccgttcga cgagattttc gaagagcttg aatcagctgg ggcgggggtg tcctttggtg
     6961 gggcgcggca cacatccggt ttctccggcc atagcctggt ggaggatttt gacttaattc
     7021 tggacgtcat cgatgaatcg ctccgtcgcc cgacttttcc ggctgaacag attgagcggt
     7081 tgcgcggtca gatcgtgacc ggcttgcaga tgagggccaa caatacgcgc cagatggcct
     7141 ggttatccta ctatgaaacg gcctatcagg ggcacccata tagccgggcg gtgggcggtt
     7201 atctcgacag catcggcaat atgaatcgag ccgatattgt cgatttctac gatcgttatt
     7261 acggccccag gaacatgatt atcggcgtag tcggcgcgat ttccgccgat gatgtgctgc
     7321 gcaagctcga gccagtcttc ggggagtggc ggaatgatca ccaacagata acgcccctct
     7381 ccccggatat cgagcgccct atcggattgc agcggcgcta cgtgccgatg ccggagaagc
     7441 atcaggccga tattgtcctg ggcttgcccg gtccgcgccg ctcggccccc gactacctgg
     7501 atgccagtgt gatgaacacg atcctcggcg tttttgggat gatgggacgt attggccaga
     7561 acgttcgcga agagcaaggc ctggcttatt acgcgtcgag ccgcctggaa ggtgggttag
     7621 gacccggctc gtggaatgcg tcggccggcg ttgcgccgga caaggtcgag caggcgatag
     7681 aatccatccg ccatgagatt cggcggatga tcgacgagcc ggtgacggcc gaagagctgg
     7741 ccgatagcct ggcttaccgc accggctcat tgccgatgag cctggagacg aacagcggtt
     7801 tggtggatat cattaccgac atggaacttt atggactggg tcttgattat cttcaccgat
     7861 ataaggacga gatcaacgcc attacggtgg agcgcatcca gaatgcggcc caaaaatacc
     7921 tgagcgccga cgacctggtc atcgctgtta gcggcccggt ggaggcgctc tgatgctggc
     7981 cgttggggtc gatatcttcg aggtcgagcg gatggcccag ggcatggaac gacatggcgc
     8041 gcggttttgt gatcgttttt tcacggaacg agagcaagcc caatgcgaag ggcggccgac
     8101 aagcctggcc ggtcgttttg ccgtcaagga agctgtcggc aaggcattgg gcaccggcat
     8161 tggcgatgtg gcctggaagg agatcgagat catcaatgac gagcgcggcc gcccacagtt
     8221 aaccctccat ggcgcggccg cgaggctggc cgatgagttg ggcctgaccg aatgggcgat
     8281 cagtctgtca cacacccgga cacatgctgt cgggatggcc gttgccctga tgccggagcg
     8341 ccggatgatc gatacaatct cgctcgaaac tcgcgcggat acccgtgcgc ctaataagac
     8401 aggtttataa atggatgata tgacgactgc gccggcccaa cgccggttac ttgtggtgct
     8461 tgcccacccg gatgatgagt ctttcggtat gggtggcacg ctggcacgat acgccgatga
     8521 aggtgttgat gtccatatcg ctattgctac cgatggcgtg gccggttcgg tagccaaggg
     8581 tcacgaggac aagttaaaag agttagccaa ggttcggcgg aacgaactgg aagctgccgt
     8641 gcgtgttttg ggtggcaagc tccacatgct gggctatcgg gattcgggat atatcggtga
     8701 tccggccaac catcatcctg aggcttttat caacagcgac tttgaggaag ccaccgggcg
     8761 cgtggtcgcc cttatccggc agatccggcc acaggttgtg gtgactcatg atgagacggg
     8821 tggatattac catcccgatc atattttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnctt cacgcccgca tcgattacag acgctactgg gatgtgaagc gcgccgccgg
     9001 agccgaacac cccagccagg gcggagggcc atcgcggagc cgtatgctgc ccgaatcatt
     9061 ccagcggcgc tatatggcga ccgagacatt tattcgcgcc tatccacccg tccccgacgg
     9121 ctttcgtgaa aacgatctgt ttgccggcgt cgacgcgacg gtatgattga gcctggcgcg
     9181 aacaagaggg aggaacgagc tatgacattt caacgagtga tgaaaatatt ggggatattg
     9241 acgggactgg cggcggccgc ggccgcgctg gttgttgctt attttacccg acgcatggtt
     9301 gcgcctgcgc gacaaccatt gtgggccaat ccgggtgata tgggcctgga gtatgaaaac
     9361 gcgcagtttc cggcgctgga cagcgtccgg ctatcgggat ggttcattcc cgcaacaaag
     9421 agcgaggatc gccatggtgc cgccattatc ctcgtccacg attggacgtg gaaccggctc
     9481 ggcgatgccg cgggcgatat gctcgccaat ttatcaggca caacacctgt cgaacttctg
     9541 cggctggcgt atacccttca ctacgaagga tatcatgtcc tcatgtatga ccagcgcaac
     9601 cacggagaga gtgcggccca tccgccggtc acctttggtc aatcggaagc taacgatctg
     9661 ctgggggcga ttgcctacct gcaaacccga tcggaggtcg atggctcgcg tattggggcg
     9721 attggtttct ccatgggggc caatgctatc ctgtatgcca tggcccagac cgatcaattg
     9781 ggcgcagcga tcgccgtgca gcccatgact atcggtacct acgtcaaggg ttataccgga
     9841 gatcttctgg ggttgccggg ccgggcttta ctgccattgg tagagacggc ttactccgca
     9901 gtgtcgggtg tgtccattgg tgcgctccaa ccgggttttg ccgtggccgg tgcggggcaa
     9961 acgccggtct tgttcgtgca atgcaaagag gatcattggg gttcgccgga tgataccgcg
    10021 cgccttcgcg ccgtgacgcc cgagggagaa gggccgctct acgtcgatgg cacccaccgc
    10081 tatgagggct atcaatacct cattgagaac ccccgcgtgg ccgttacctt ttttgaacgg
    10141 catatctaac cgttgaagca gactaaagta aaaaagggcg catcttgata gctgcgccct
    10201 tttttattaa tccccgggtt ccattaactc cagttcgttt agatcgtctg ggagatcttc
    10261 ctccgtgctt tttcgccgga actggctcat atacaggtca aagtaaaagc ctctcttttc
    10321 cagcagcgag cgatgtgtcc cttgttcgat gatctgccca tctttcagaa ccagcacctg
    10381 atcggctgtg cgaatcgtgc tgagtctatg ggcgatgaca aaacttgttc gcccttgtag
    10441 aagctcttcc agtgctttct ggatttcgcg ttcggtgcgc gtgtctacgc tgctggtggc
    10501 ctcgtcgaga atgaggatac gtggatcgat aagcgccacg cgcgcgatgg acagcaattg
    10561 tcgctggccc tggctgagac ccgcgccgcg ctcgcccagc acggtctggt agccgtcttg
    10621 caggcgattg atgaaatcat cggcgtgggc cagttgggcg gcggcaatta cttcctcgtc
    10681 ggtcgcgtcg ggtcgcccat agcgaatatt gttcatcaca gtatcgctga acagaaatga
    10741 atcctgcaaa acgatgccga tctggcggcg caggctttgc tgagtcacat cacggacatc
    10801 gattccgtct atcgtaatgc tgcccgattc tacgtcccaa aagcggggca gcaggttgat
    10861 gatggtcgtc tttcccgcgc cggttggtcc cacgatggcc acgctctgac cgggtttggc
    10921 atcgatggat atgccccgca ggataggctc gtcgggattg tactcggcga agacgtcctg
    10981 atacaccaca tgcccggtga taggcggcat cacgatggca ttcggcttgt ccttaatttc
    11041 gacaggttcg tccagcagac caaagatacg ctcagcgccc gctatggcac tctggatcgt
    11101 ggcccagagg gttgagattt gctggacagg gcggttgaat tgctgggtat agccgatgaa
    11161 ggtgatgatt aggccgaccg agatcgtctc tcccattaac ccgccaccgc gtaacatgag
    11221 cagaccaccg atgccggcaa ctaccgccag gctgacgtag ctgagacctt ccagggcggg
    11281 gcctaacgcg ccggtgtaag cctgggctcg aatgttggcg tctcggttgg cggcgttgct
    11341 ctcgcggaag ttctggatgt tctcttcttc ccggctgaat gcctgaactt cgcgcacggc
    11401 cgagatactt tcttgcaggt cggcgttcac gttgccgatc tgttgccgcg ctcgccgaaa
    11461 tgccttgcgg gcctgctctg agaaccaacg ggtgatgatg accatcaacg gtaaggtgac
    11521 aaggctgatc agggcaaacg gcgcgttgcg ctggaacatc acgacgatga tccagccgat
    11581 gagcaggatg ccctgcaaga cagaaacgag gccgaagccg atgacttgct gcaatgtgtc
    11641 ggagtcattg gtaaagcggc tcatcacatc accggcctca tgtttggtga aaaagccaag
    11701 cgaaagctga tgaatgtgat tgaacacctg tgcctgcaag tcacgcaaga catggtaacc
    11761 ggcccagctc atcatgtaga acatgaagcc gctcaggacg gcgctgccga catagaggcc
    11821 gacgatgagc aggataagcc gaccgaagcc cgccagcgtt tccccggcag tcgcggttat
    11881 atcgggcgtg gtataccagc aattggagaa cgcgttcgcc tgttccgcat tatccgcgaa
    11941 agagcctaac gctgctccgc caccggcagc cgcggccatg ctgtaaggcc ccagatagca
    12001 gtctaccgcc tgaccggtca ggtcggggat gagcacttgc atgtaggtgc tgctgattac
    12061 caacacccca accaggagaa gtatcaggcc atagcgctgg aaataatgcc acagccgaac
    12121 cagcgtcccg cgcacgctgc gggccttgct ggtctccatg ccaagaaatt gtgattgtcg
    12181 atccatcata atggtttctc tgtaatcgtc attggataac gcgcaacagc cgaatgcctt
    12241 aattgaggtc tggctgtgcc gatgccggag cgatatcctc cgctaattgg gagtggtaga
    12301 tttcagcgta gatagggctg gcttccatga gttcttcatg cttgccgcga gcgacgatgc
    12361 tccctttgtc gagaaccagg atgagatccg cgttgagcac ggtgctgatg cgctgggcga
    12421 tgacgaagct ggtgcgtccc ttcatcaatt gatcaagtgc cttctgaatc tcgtactcgg
    12481 tgttcaggtc gacgctgctg gtgctgtcgt cgagaatgag gatgtgcggg tccatgagca
    12541 aggcacgagc aatggctacg cgctgctttt ggccgccgct gagtgtcgcc ccgcgctctc
    12601 caaccggtgt gtcataccct tgcgggaagc cggagatgaa atcgtgagcg gcggccgcct
    12661 tggctgcggc gatgatctcg tcgtcgctcg catcgggacg gccgaaggcg atattatcgc
    12721 ggatggaacc gctgaacagg acagtctctt gcaacacgat gccgatatgc tgtcgtaggc
    12781 tctccagcgt cacatcgcgc acgtcatggc tgtcgatcag cacctggcct tcactgacat
    12841 catagaatcg cgggataagg ttgatgattg tacttttacc gctgccggtc gcacccagga
    12901 gagccacagt ttggcccggt tcggcggtga aggcaatgtg actcagcacg gcttcgccac
    12961 cggggaagta tcgaaaagat acatccttaa attcgacccg gccgtctatc tctcccagat
    13021 cgatggctcc gggtttgttt tcgacgtcgt tcttggcgtc cagtatctcg aaaatccggt
    13081 ctgcgctggc gcccgcctga gccatcaggg agatgatgaa tccgagttgc cccatgggaa
    13141 tgaagacgta gacaaggtag aggctgaact tctgccactc accgagcgtg agcgtgccgt
    13201 cgatgatctg tcgcccacca aagtagagca ctgccccctg acctaaattg gcaatgagga
    13261 agatgaatgg gaacagaaag gaaaaaacac gactcacctt gatccgttgg tccagatacg
    13321 aatcaatatt ctcatggaag cgcttttctt cacgctcttc ggccgtgaat gccttgacta
    13381 ccttcagccc ggccagattc tcctgcaacg tcgtgttgac ccgccccagg cgtttctgca
    13441 cttcgacgaa cagcggctgg gcgatgatgc cgaacagcat gaaaataccg agcgcgagcg
    13501 gcagcaccgg caggacaaca agagtcagcc gaatattggt ccaggtcagg atagccagcg
    13561 tcccaaccag caatatgagc gcctgcaatg ccatcagcag accctgaccg atgaagactc
    13621 gcaggcgttc cacgtcatcg gtggcccgga tcatcaattg tcccgttcgg ttgcggtcgt
    13681 ggtagctaaa gctgaggcgc tggatcttgg tgaagatgtc gttgcgcagg tcgaaggcga
    13741 tgctttcgga gagggcctgg gacatgaagt tttgtccaaa ggcgaacaac ccgcgcgcga
    13801 cggcgaacag gacgatgatg agtgcagccg tgatgagcgc cctgggcgcg gcatcaagct
    13861 cggcttgggc ggcaacaagg tccaggccta atttttgggc cgcgatagct tgaatgttgg
    13921 ccgggagatt gagtatccct tgatgcgtcg cgctggtgac gatcgtgtcg ataatgcgct
    13981 gtaatagctg tggcacgacc agttgcgctg ccgtggcaat gatcaaggca acggttgctc
    14041 cggctgttag ccgggggtat tttgtcagat agtttagggc gcgaccgagc gattgcaggt
    14101 tcgcgcgtcc gccggccttt tgcttgcccg cttgctgact tcctgaaagc tgcatattgg
    14161 actccgtttg agtggattaa ctattctttt agtgtgctga tttggtcgct gagtttatgg
    14221 agaagagcgc tcagtgtttc aatctcctgc ggagatagcg cggagaagag atcggcgatg
    14281 agctggaaat gctggtgctc gtgttcgtaa gccagattcc ggccggcatc actcaggtga
    14341 atgttgaatc gacggcgatc atcgctgtcc agttgtcgct caatgtaacc
//
LOCUS       DAOMQG010000046        14367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1820_length_14367_cov_9.606274, whole genome shotgun sequence.
ACCESSION   DAOMQG010000046 DAOMQG010000000
VERSION     DAOMQG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14367
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_14367_cov_9.606274"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="PLC06_04390"
     tRNA            complement(2..74)
                     /locus_tag="PLC06_04390"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Glu,seq:ctc)
     gene            complement(147..1466)
                     /locus_tag="PLC06_04395"
     CDS             complement(147..1466)
                     /locus_tag="PLC06_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRO89715.1"
                     /translation="MESQLLFEISILIFLILLNGVFALSELAIVSSRRERLQMLVDEG
                     NKGASIALEMAQEPTALLSTVQVGITLIGILAGAFGGASLSDELAMLVAPIPVIGAYA
                     RTVSLAIVVGTITYFSVVLGELVPKRLALRDPERIAAVVARPMGFLSRLARPLVRLLT
                     LSTAFFLRVLGVRDSIGESTITEEEIKVLVEQGALAGVFEEAERDMVESIFRFGDRQL
                     RSIMTPRTEIVWLDINDPEEIIRETVSLSHHSRFPVCDDSIDRVLGIAEAKDLLSNSW
                     SNEPFDLRAVMKTAVFLPETMPALRALEKFKKTGNQSALLVDEFGGIEGMVTLIDMME
                     AIVGDIPTLEEIAEPPVVRREDGSLLVEGYLDVEDLKELLDVDELPDEEDYQTLGGFV
                     VLRLGRLPRAGDIVEWHNYRFEIVDMDGNRVDKVLIMPRAQKSAAEG"
     gene            1729..4041
                     /locus_tag="PLC06_04400"
     CDS             1729..4041
                     /locus_tag="PLC06_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRO89716.1"
                     /translation="MDLLAKLNPAQREAVSAPPGALLVVAGPGSGKTRVLTHRIAYLI
                     REMHVAPWNILAVTFTNKAAREMEHRIEQLLDGRPQGLNMGTFHAICARLLRRETDNL
                     AAYQRDFVIFDTADQLQVIKQALSELNIDDKKFPPGKMLNGISSAKNDLITPDEYQAT
                     NYAGAVIQRVYQRYQLTLQVNNAMDFDDLLMNTVLLFDERPDVLARYQARYHHILVDE
                     FQDTNTTQYRLLCQLAGQGNSIFAVGDSDQSIYKWRGADYRNIENFYRDFPQSRTILL
                     EQNYRSTQLILDAAIEVIRRNRDHIPKKLYTERNGGAKIILREAYNESDEAATIVATI
                     ESLMLEGYNGNDFAVMYRTNAQSRALEEAFVQAGVPYRLVGATQFYQRREIKDIIAYL
                     RVVHNPLDAVSFNRVLNVPARKIGQQTQMQFAAWAAALGLQPAEALVKLATDEDVQHP
                     FTRQAYTALFTFGSLLTSWLALRERVSVGELLDAILDQVNYRAWLEDGTEEGEERWAN
                     IMELRGAAGIDGELSLGDFLQQVSLVAETDNLDPDAQATTLLTLHAAKGLEFPVVFIT
                     GLEEGLLPHSRSLDDEEELAEERRLLYVGLTRARDALYLSHAFRRTSWGDSSVAAPSR
                     FLADIPEDLVDGRTTGNRRRESIDRASSWNSGRTSWGQSPGSPSSAAGRGNSNRRPSH
                     VTNTGANNSGAFDNKPLPTPNRSGDEPPAGRSVTAQFKTGQRVRHGKFGEGTVIESKV
                     TGGDEEVTVAFPGVGIKRLAAGIAKLEHLE"
     gene            4046..4663
                     /locus_tag="PLC06_04405"
     CDS             4046..4663
                     /locus_tag="PLC06_04405"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRO89717.1"
                     /translation="MNYLIDGHNLIARTPGLSLSDPDDEAKLVQLLRRWTAADARRKV
                     TVIFDGGLPAGEARHLSGGGVKAIFAPNNSSADAVIIRRIEAIGNPPEFVIVTSDHAV
                     ANAAARRRIAVQPSEVFAANMMSDRRFGGSENADTQRPEPPAMNPNEVQEWLSLFGPE
                     PETPRPARPARKPAPSQPVKPKKKTEEEKAADDIDEWLRLFGYKE"
     gene            complement(4667..5725)
                     /locus_tag="PLC06_04410"
     CDS             complement(4667..5725)
                     /locus_tag="PLC06_04410"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRO89718.1"
                     /translation="MDFDPTLHPHRRRNPLTGEWVQVSPHRLQRPWQGHVESVPAESR
                     PAYDPACYLCPGNERAGGLRNPAYGDTYVFANDFSAVLPDVPSTEEPPARGPEQLLWA
                     RAVPGTARVMCFSPRHDLTLPEMDLPAIRRVVEMWVEQTADLGRRYRWVQLFENKGEI
                     MGCSNPHPHGQVWGIDTLPNEPAKEDRQQRIYFEAEGRPMLLDYAELESERRERVVVE
                     NEDWLVVVPFWAVWPFETMLLPRRRGIPRLPDTTADERRSLAEILKRLLIRYDNLFNV
                     SFPYSMGWHGAPFTTEPVSHWQLHAHFMPPLLRSATVKKFMVGYELMAEAQRDLTAEQ
                     AAARLRDLPETHYKARPA"
     gene            6117..7697
                     /gene="murJ"
                     /locus_tag="PLC06_04415"
     CDS             6117..7697
                     /gene="murJ"
                     /locus_tag="PLC06_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRO89719.1"
                     /translation="MTTPSPIPMTLIETEKPARRVRLGGITALAQSAWMGFPLGSEIL
                     EPLRLAPAALRRWTQIVRPHRPAGLSLRGLFRREFGIAEASFLLMASFFTSAALGSVR
                     QVLFNAQFGVSMEANAYYAAFRLPDTLFSLVAGGALSSAMIPVLLTTRQDEGESAAWQ
                     LVSLVLTALLAFMALIIVIVGLFTPFFIKNLLAPGFDPETSALTVTLTRIMLIQPLIL
                     AVGSVATAVLNSRNQFFPTALSVVSHNVALIIGILASSAIDGLGILGPTLGVIGGATL
                     QMLILLPGLFNRDGHIAMAFNFADQRLREVIRLLIPNGLSVGVNYSGFIVDTSFATRA
                     PEIAGLAALYNAFLLVGLPIALLGQAVGQAAFPRLTAQAESGNWRQMRRTQLTSLAAA
                     VVLALGAVAGLVLLGRPIIRLLFERGAFDAAAGDLTYRVLVVYAIALPAYVATEVITR
                     GLIALRDTRTPLLTNTGQLLGRIILIALLIESLGVVAIPAAFALTSTLETLALGGALW
                     LKLRRRIAESTAATERPS"
     gene            7694..8323
                     /locus_tag="PLC06_04420"
     CDS             7694..8323
                     /locus_tag="PLC06_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89720.1"
                     /translation="MNDDKLSRPDRIARIETELWEAYYNREWLREFRLLITMHREFFH
                     MNTPTAVLASYDAARAAMAFAPLDANDLPQARGYLLRYYEKVRQALGCRATANDLAER
                     ELHYWIVHREVARGRLAEIKTGRAIDDPDDAEIEPVINAFANLHAGLFNSTPEAMHES
                     AVYRARAAAIVDRISGRYSPDVAADWARVETELRLAYRAIEQATEIVAA"
     gene            8320..8577
                     /locus_tag="PLC06_04425"
     CDS             8320..8577
                     /locus_tag="PLC06_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89721.1"
                     /translation="MNRQIIRPELETQHAADVSTEAAGLTFGPFSAEWRAIGKDVLIE
                     IAAGYYLGRACVRWWWGGQRVAYFILRHESEQKLSSVRSVE"
     gene            8644..9141
                     /locus_tag="PLC06_04430"
     CDS             8644..9141
                     /locus_tag="PLC06_04430"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRO89722.1"
                     /translation="MKEYRFVDHWYIKAPIEVVYNHVSDPRTYVSWWPSYDGVRILKD
                     VPYPHVGGQAELIVKSPFGYRLRIEVETTEATPPTYLKTVSRGQLAGTGIWEFSQEGD
                     TTHAVWTWIVRSDHPVLNKLEWIAKPIFALSHVLASGKGHRGLKKYLETNPQFDHEPQ
                     RGAIQ"
     gene            9138..9959
                     /locus_tag="PLC06_04435"
     CDS             9138..9959
                     /locus_tag="PLC06_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89723.1"
                     /translation="MTNKTFTRFGGLSAIIVGLLSILYAIFFLIISRQNESLGATGSW
                     IILAMSGIFSSAAFVAFYERLQPVFKGANMNADKIQENPITGMRTIVIETPEETGGRR
                     VVVEYQLQPGTGRGYTVAHIHRRYVERFEILSGRAAYVLNGVTGAAIAGETVIVPIGT
                     SHVHPWSVGDEPLVVRQTTEAVAPDVTGLNSALMAAETLIDLSHQGKVNANGQPNPLQ
                     AAVIFHDLLLPHSHLAGLPFAAQRVIFGVLSAVGRLLGYRAVYPAGTIVGTSAVQ"
     gene            10061..10627
                     /gene="thpR"
                     /locus_tag="PLC06_04440"
     CDS             10061..10627
                     /gene="thpR"
                     /locus_tag="PLC06_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRO89724.1"
                     /translation="MNTIRTFIAVQLTDEVRDALREVVRSLSGRVERGAVRWVRPEQM
                     HLTLRFLGDSDTGKLPAVQAAMDAVAASNASFEMRLGGIGCFPNRNRPRVIWVGLSGE
                     EARLLSLVVALEEELAHLGWDPEGKPFRAHLTLGRVKDERGAQGIEWAADVQPLAIPV
                     KAIHLIESQLRPTGPVYTLRHSSRLALG"
     gene            complement(10727..12007)
                     /gene="tyrS"
                     /locus_tag="PLC06_04445"
     CDS             complement(10727..12007)
                     /gene="tyrS"
                     /locus_tag="PLC06_04445"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRO89725.1"
                     /translation="MATTIIDELRWRGLIYDKTEGLDELTSAGKITLYNGFDPTKDTL
                     HVGHLVPMMQLARWQRYGHSPIALAGGGTGMIGDPGGRSTERNLLSLEEIDANVVHIK
                     TQLESILDFEVKSNPAKVVNNADWLRRLSLVDFLRDTGKHFTVNYMMAKDSVKSRLER
                     EDGGISFTEFSYMLLQAYDYLHLFDVEKCALQAGGSDQWGNILTGVELIRRVRGSRAH
                     ALVYPLITRADGTKFGKTAGGTSVWLSPEYTSPYRFYQFWYNTDDADVVTYLKYFTWL
                     DEGRIAELEHLLFDQPERREAQRTLAREVTRMVHGETALAKAEQASQALFGGDVTGLD
                     AADIEDIFAEVPSNEVAKEALSGGLAVVDLLVTSGLANSKADARRAIQGGGIYLNNER
                     VTDATQAADLSQAIDGRFLILRKGRRQYHLVRVR"
     assembly_gap    12108..12207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12208..13400)
                     /locus_tag="PLC06_04450"
     CDS             complement(<12208..13400)
                     /locus_tag="PLC06_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO89726.1"
                     /translation="MDDRIIEFVRGMRAAGVRVSMAESADAMRAIEVLGITDKDLFRQ
                     SLRTTLIKESEDFAVFEELFPLYFSAGGPPMQNAMEDLSEDEQEMLQAALQALSGRLQ
                     ALMDWLTSGEGPTKEELEQMAERAGVQWANNQQEARWVTRRMLQQMGMAQLEDQLREL
                     MERLAEMGMSQEAIEKLMGVVEANREALTEQMAQSVGRRIAEQRANRPDDLYGSDLMN
                     KPFEALSQEEANLLRKEVQRLVTQLRSRAALRRKRGKEGRFDSKGTIRANQRYGGVPI
                     ELKFKRKKLKPSLVLICDISRSMLSVAEFMLRLTYELQDQVAKTRSFGYYSDMEEISL
                     TMAGNRPAEAVDGVMERFYGRAPQHYATDLGHSLGTFYHNWLDSVNNRTTVVILGDGR
                     NNFNDP"
     gene            complement(13422..14345)
                     /locus_tag="PLC06_04455"
     CDS             complement(13422..14345)
                     /locus_tag="PLC06_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRO89727.1"
                     /translation="MFKDRADVGRRLSGQQYIPSDEIATVIYLAQSLCKPILAEGPAG
                     VGKTELAKAWAAALGAPLVRLQCYEGLDESKALYEWEYAKQMLYTQLLRDTLHNVLGD
                     VSSLAEAAGRLAREEDVFFSEHFLLPRPLLKALTSDEPVVLLIDEIDRADVEFEAFLL
                     EVLSDFQVSVPELGTIRARHQPMVLLTSNNTRELSEALKRRCLYLYVGYPTLEAELDI
                     VHLKVPDLKPDLARQAVATVQQLRNMDLRKVPSISETLDWARALVALNAQSIDEETMS
                     NTLTVLLKYEADVQKAKRMMGGGGRSRPGGR"
BASE COUNT         2854 a         4578 c         4076 g         2759 t
ORIGIN      
        1 atgccccata ggggactcga acccctgttt cggccttgag agggccgcgt cctgggccac
       61 tagacgaatg gggccagcga cggttgattc tagcagagac aagggtgagt gtcaaacggc
      121 cagccaaatc aaaagccaca actccatcaa ccctccgcgg ccgacttttg cgccctcggc
      181 atgatcaaaa ccttatcaac gcgattgccg tccatgtcga caatctcaaa gcgatagtta
      241 tgccactcca caatatcgcc cgcgcgtggc aggcggccaa gccgcaggac aacgaagccg
      301 cctaacgtct gataatcttc ctcatcgggc agttcatcga cgtccagcag ctccttcaag
      361 tcctctacat cgagatagcc ttcgacgaga agagatccat cttcgcggcg caccaccggc
      421 ggctcagcga tctcttccag cgtagggatg tcgccgacga tggcttccat catgtcgatg
      481 agggtgacca tgccctcgat gccgccgaac tcatcaacca gcaaggcgct ctgattgcct
      541 gtcttcttga atttttccag cgcccggagc gcgggcattg tttccggcag gaaaacggcc
      601 gttttcatga cggctcgcaa gtcaaacggc tcgttggacc aactgttgga gagcagatcc
      661 ttggcctccg cgatgccaag tacccgatcg atcgaatcat cacacacggg gaaacgcgaa
      721 tgatggctta ggctcaccgt ctcgcggatg atctcttcag ggtcattgat gtcgagccac
      781 acgatctccg ttcgcggcgt catgatcgag cgcaactggc ggtcaccgaa ccggaagata
      841 ctctcgacca tatcccgttc cgcttcctca aagacgccgg ccaatgcgcc ttgctcgacg
      901 agaaccttga tctcctcttc cgtgatggtc gattcaccaa tcgaatcacg gacgcccaga
      961 acgcgcagga aaaaggcggt ggataatgtc agaagcctga ccaacggccg ggcaagtcgg
     1021 gacaagaagc ccatcggccg ggcgactacc gccgcaattc gctccggatc gcgcagcgcc
     1081 agccgcttgg gcacaagctc gcccaatacg acgctgaaat acgtgatggt tccgacgacg
     1141 atggccagcg agaccgtgcg ggcgtaggcc ccaatgaccg ggattggcgc cacaagcatg
     1201 gccagctcgt cggataacga cgcgccgcca aacgcgcccg ccaggatacc aatgagcgtg
     1261 atacctacct gcacggtgga gagcaaggcc gtcggctcct gggccatctc aagagcgatg
     1321 gacgcgcctt tgttgccttc atcgacgagc atctgcaagc gctcgcgccg cgatgagacg
     1381 atagccagct cggagagcgc gaatacgcca ttgagcagga tcaagaaaat gaggatgctg
     1441 atttcaaaca gcaattggga ttccataagt tgtcagtttg ctgattttcc ggcgaatcga
     1501 ccaacgcgcc aaacaaacca ggccttccgc cgcctgccct cgctatcgga gcgatggatg
     1561 acagttacaa aagcggtcga cctccacaca gagtataaac catcggccaa taattttgcg
     1621 aaaacagact cacgattgac cctccgctcg cggaatttca cctaacgccg gatcaacgga
     1681 ttccccttta aggctgaccc aatcggcccg atgtaataga attgaggcat ggatttacta
     1741 gcgaaactga acccggccca gcgcgaggcc gttagcgccc cgcccggcgc gcttctggtc
     1801 gtggccggcc ccggcagcgg caagacccgc gtgctcaccc atcgcatcgc ctatctcatt
     1861 cgcgagatgc acgtcgcgcc gtggaatatc ctggccgtca ccttcaccaa taaggccgcg
     1921 cgcgagatgg aacatcgcat tgagcagctg ctcgacgggc ggccgcaagg cctcaacatg
     1981 ggcacgttcc acgccatttg cgcccgcctg ctgcgtcgcg agaccgataa tctggccgct
     2041 tatcaacgcg acttcgtcat cttcgacaca gccgatcagc tgcaggtgat caagcaagcg
     2101 ctatcggaac tcaatatcga cgacaaaaag ttcccgccgg gcaagatgct caacggcatc
     2161 agcagcgcca aaaacgatct catcacaccg gacgagtatc aggccactaa ctacgccggc
     2221 gcggtcatcc agcgcgtcta ccaacgttac cagttaacct tgcaggtcaa caatgcaatg
     2281 gatttcgatg acctgctgat gaacaccgtg ctgctcttcg acgagcgacc tgacgtcctg
     2341 gccaggtatc aggcgcgtta tcaccacatc ctggtcgacg aatttcagga taccaacacc
     2401 acgcaatatc gtctgctttg ccaattggcc gggcaaggga acagtatctt tgccgtgggt
     2461 gattcggatc agtccatcta caaatggcgt ggggcagact atcggaacat cgagaatttt
     2521 taccgtgatt ttccccagtc gcgtaccatc ctgctggagc agaactatcg ctccacccag
     2581 ctcatcctcg acgcagccat cgaagtcatc cgccgcaatc gggatcatat ccccaagaag
     2641 ctctacaccg agcgcaacgg cggggcgaag atcatcctgc gcgaggccta caacgaatcc
     2701 gacgaggccg cgacaatcgt cgccaccatc gagagcctca tgctggaagg gtataacggc
     2761 aacgatttcg ccgtgatgta tcgcactaac gcccaatccc gtgccctgga agaagccttc
     2821 gtgcaggcgg gcgtacccta ccggctcgtg ggcgcgaccc agttctacca gcggcgcgag
     2881 atcaaggaca tcatcgccta cctgcgtgtc gtccataatc cgctggacgc ggtcagcttc
     2941 aaccgcgtgc tgaacgtgcc ggcgcgaaag atcggccagc aaacccagat gcaattcgcc
     3001 gcctgggcgg ccgcgctggg tttgcaaccg gctgaggcgt tggtgaagct ggccacggac
     3061 gaggatgtgc aacacccgtt cacccgccag gcctacaccg cactgttcac cttcggcagc
     3121 ctactgactt cgtggctcgc gttgcgcgag cgggtctccg tcggcgaact gctggacgcc
     3181 atcctggatc aggtcaacta ccgcgcctgg ctggaagacg gcacggaaga gggcgaagag
     3241 cgctgggcca atatcatgga gcttcgcggc gcggccggca tcgacggcga gttgagcctt
     3301 ggcgatttcc tccaacaggt ctcgctcgtc gccgaaacgg acaacctcga tcccgatgcg
     3361 caggccacga ccctgctaac gctccatgcc gccaaggggc tggagttccc cgtcgttttc
     3421 atcaccgggc tggaagaggg tctgctgccc catagccgct cgctggacga cgaagaggag
     3481 ttggccgaag agcgccgcct gctctatgta ggcctcactc gcgcgcgcga cgcgctctac
     3541 ctgtcccacg ccttccggcg cacgtcgtgg ggcgatagtt ccgtggccgc gccttcccgt
     3601 tttctcgccg acatccccga agatctcgtg gacggacgga cgaccggtaa tcgtcgccgc
     3661 gagagcatcg accgggccag ctcgtggaat tcagggcgga cttcgtgggg ccaaagtccc
     3721 ggctcgccct cgtcggccgc cggccgcggc aacagcaatc gccggccatc ccacgtcacc
     3781 aacaccggcg ccaacaacag cggcgcgttc gataacaaac ctctgccgac cccaaacaga
     3841 agcggcgatg aaccccccgc agggcgatcc gtgaccgcgc agttcaagac cggccagcgc
     3901 gtgcgtcacg gcaaattcgg cgaaggtacg gtcatcgaga gcaaagtgac cggcggcgac
     3961 gaagaggtga cggtcgcttt ccccggtgtc ggcatcaaac ggctggcggc cggcatcgcc
     4021 aagctggagc atctcgagtg agtagatgaa ctatctgatc gatggtcata acctcatcgc
     4081 ccgcaccccc ggcttgtcat tatccgaccc cgacgacgag gccaagctgg tgcaactgct
     4141 gcggcgctgg acggcggccg acgcgcgacg caaggtgacg gtcatcttcg atggcggtct
     4201 gcctgccggg gaagccaggc atctgtccgg cggcggagtg aaggcgatct tcgcccccaa
     4261 caactcgtcg gccgacgccg tcatcattcg ccgcatcgag gctatcggca atccaccgga
     4321 attcgtgatt gtcaccagcg accatgccgt ggccaacgcg gccgcgcgtc gccgaatcgc
     4381 cgtccagcca tccgaggtgt tcgccgcaaa catgatgtcg gatcgccgtt tcggtggaag
     4441 tgaaaacgcc gatacccaac gtcccgaacc gccggccatg aaccccaacg aggtgcagga
     4501 gtggctgtcg ctcttcggcc cggagccgga gacgccgcga cccgcccgcc ccgcgcgaaa
     4561 acccgcccca tcccaaccgg ttaagccgaa aaagaaaacc gaggaagaaa aagcggccga
     4621 cgatatcgac gaatggctgc gactgttcgg ctacaaggag tagatgctac gccgggcgag
     4681 ccttgtagtg cgtctcgggc aaatcccgca gccgcgccgc cgcctgctcg gcggtcaggt
     4741 cccgttgtgc ctcggccatt agctcatagc cgaccataaa cttcttgacc gtcgccgacc
     4801 gcaggagcgg cggcatgaag tgggcgtgca gctgccagtg gctcaccggc tccgtcgtaa
     4861 acggcgcgcc atgccagccc atcgaatagg ggaatgacac attaaacagg ttatcgtagc
     4921 ggatgagcaa tcgcttgagg atctcggcaa gcgatcgtcg ctcatccgcc gtggtgtcgg
     4981 gcagacgcgg aataccccgg cggcgcggca ataacatggt ctcgaacggc cagaccgccc
     5041 agaacggcac gacaaccagc cagtcttcat tttccaccac cacccgctct ctccgctcgc
     5101 tctccagttc ggcataatcc agcaacatcg gccgcccctc ggcctcaaag tagatccgtt
     5161 gctgcctgtc ttccttggcc ggctcattgg gcagcgtgtc gatcccccat acctgcccgt
     5221 gggggtgcgg gttcgagcaa cccatgatct cgcccttgtt ctcgaaaagc tgcacccagc
     5281 gatagcgccg ccccaggtcg gccgtttgct cgacccacat ttcaactacc cggcgaatag
     5341 cgggcagatc catctccggc agcgtcagat cgtggcgtgg cgaaaagcac atcacccgcg
     5401 ccgtgcccgg cacggcccgc gcccatagca gttgttcagg gccacgggcc ggcggttctt
     5461 ccgtcgatgg aacgtcgggc agcaccgcgg agaagtcgtt ggcgaaaacg taggtgtcgc
     5521 cgtaggctgg attgcgcaac ccgcccgctc gctcgttgcc cgggcacaga tagcaggcag
     5581 ggtcatatgc cgggcgactc tcagccggta ccgactcaac gtgcccttgc cagggacgct
     5641 ggaggcggtg gggcgaaacc tgaacccact cgccggtcag cgggttacgg cggcggtggg
     5701 ggtggagagt cgggtcgaag tccatggcat gtaggatagc aaaaaaactg cctgtcctcc
     5761 atatgttata gggaatttga aacttgcctc tatgggacag cgctcgcttg cccactcatg
     5821 tttccagatt gccgatttca cggctccaca agcatgacgt gatctcctcg atgcccgacc
     5881 gcaggtggcg ccgataggtg ccgaacggga gatcaagcag ttcggccgcc tgctcctgag
     5941 tggtgaccgg ctgcaggatt gatcacaaac tgaacagaaa atggtctgta tttcagggca
     6001 atggatcgat taagatgcca gcattcacag gatgccccat tccaaatggc ccgcatcatt
     6061 gacaggcgaa tggaacaacg atttgaacac cgaaatttat ctggcgcgtt cgtaacatga
     6121 ccacaccttc acccatccct atgaccttga tcgagaccga aaagcccgcc aggcgagtcc
     6181 gcctgggcgg tatcacggcc ctggcccaaa gcgcctggat gggcttcccg ctgggtagcg
     6241 agattctgga gccgctccgg ctggctccgg ccgcgttgcg gcgctggaca caaatcgttc
     6301 ggccgcatcg gccggccggc ctgagcctgc gcggtctatt ccgccgcgaa ttcggcattg
     6361 ccgaggccag cttcctgctc atggcgtcat tcttcacctc ggccgcgctc ggctccgtgc
     6421 gccaggtgct ctttaacgcc caattcggcg tcagcatgga agccaacgcc tactatgccg
     6481 cctttcgtct gcctgacacg ctgttcagcc tcgtggccgg tggagcgctc tccagcgcca
     6541 tgatccccgt cctgctgacg acccggcagg atgagggtga gtcggccgca tggcagctgg
     6601 tcagcctggt tctgacggcg ctattggcgt tcatggctct cattattgta attgttggcc
     6661 tattcacccc cttcttcatc aaaaacctgt tggcccccgg cttcgacccg gagacgagcg
     6721 cactaaccgt cacattgacg cgcatcatgc tcatccagcc gcttattctg gcggtgggca
     6781 gcgtggcgac ggccgtgctc aacagtcgca atcagttctt tccgacggcc ctctcggtgg
     6841 tcagccacaa tgtcgctctg atcatcggca tcctggcttc atccgccatc gacgggctgg
     6901 gtatcctggg gccgacgctg ggcgtcatcg gcggggccac gctgcaaatg ctcatcctgc
     6961 tgccgggtct gttcaaccgc gacggccaca tcgccatggc cttcaacttc gccgatcaac
     7021 ggctgcgcga ggtcattcgc ctgctcattc ccaatggtct ctccgtcggc gtcaactact
     7081 ccggcttcat cgtcgataca tcattcgcca cccgcgcccc cgagatagca ggcctggcgg
     7141 cgctctataa cgccttcctg ctcgtcggcc tgcccatcgc gttgctcggt caggccgtgg
     7201 ggcaagccgc cttcccgcgt ctgaccgccc aggccgagag cggcaactgg cgacagatgc
     7261 gccggacaca actcacatcg cttgctgccg cagtggtgct ggcgctgggg gcggtcgccg
     7321 ggctggtgtt gctcggccgc ccgatcatcc gcctgttgtt cgagcgcggc gcattcgacg
     7381 ccgcggccgg cgatctgacc taccgggtgc tggtcgtcta cgccatagcc ctgcccgcct
     7441 atgtcgctac cgaggtcatc actcgcggcc tcatcgctct gcgcgacacg cgcacgccac
     7501 ttctgaccaa taccggacaa ctactgggtc gcattatcct gattgcattg cttatcgagt
     7561 cgctcggtgt cgtcgccatc cccgccgcct tcgccctgac cagcacgctg gagacgctgg
     7621 ctctcggtgg agcgctatgg ctcaaactca ggcgacgcat cgctgaatcc accgccgcga
     7681 cggagaggcc atcatgaatg acgacaagct cagccgtcca gaccgaattg cccgcatcga
     7741 gaccgaattg tgggaagcct actacaatcg cgaatggctg cgcgagtttc gcctgctcat
     7801 caccatgcac cgcgagttct ttcacatgaa cacgcccacg gccgttctgg cgagctacga
     7861 cgcggcccgc gcggccatgg ccttcgcgcc actcgacgcc aacgatctgc ctcaggcccg
     7921 tggctacctg ctgcgatact atgagaaagt gcggcaggcg cttggctgtc gggcgaccgc
     7981 aaatgatctg gccgagcgcg agctccacta ctggatcgtc caccgcgaag tcgctcgcgg
     8041 ccgcctggcc gagatcaaga cggggcgcgc gatcgacgac cccgacgacg ccgagatcga
     8101 gccggtgatc aacgccttcg ccaatcttca cgccgggctg ttcaacagca cgccggaggc
     8161 aatgcacgaa tcggccgtat accgcgctcg cgccgcggcc atagtcgatc gcatttccgg
     8221 tcggtactcg ccggacgtgg ccgccgactg ggcgcgagtg gagacggaat tgaggctggc
     8281 ctatcgcgct atcgagcagg caacggagat agtcgccgca tgaaccgcca gatcattcgc
     8341 cctgagcttg aaacgcaaca cgccgccgat gtctcgaccg aggcggctgg acttacattc
     8401 gggccattca gcgccgaatg gcgagcgatc ggaaaggatg tactgataga gattgccgcc
     8461 ggctactatc tcggccgcgc ctgcgtgcgc tggtggtggg gggggcagcg cgtggcctat
     8521 ttcatactac gacatgaaag tgaacagaaa ttgagcagcg ttcggtctgt cgaatgaccg
     8581 gcccgtcatc gtattctatg ctcgttgagc agcattcata aacatctata tgaggttatc
     8641 cccatgaagg aatatcgttt tgtcgatcac tggtatatca aagcgccgat cgaagttgtt
     8701 tacaatcacg tctccgatcc acgaacctat gtcagttggt ggccatccta cgatggcgta
     8761 cgcatcctga aagatgtgcc ctatccccac gtcggcggac aggcagagct gatcgtgaag
     8821 tcccccttcg ggtatcggct acggattgaa gttgaaacga ctgaagccac cccgccgacc
     8881 tatctcaaga ccgtttctcg cggccaattg gccgggaccg gcatctggga gttctcgcag
     8941 gaaggagaca cgactcacgc tgtctggacg tggatcgtgc gctccgatca tccggtactc
     9001 aacaaactgg aatggatcgc caaaccgatc ttcgccctca gccatgttct ggcctcggga
     9061 aaaggacatc gcggcctaaa gaagtatctg gagacaaatc cacagttcga tcatgagcca
     9121 caacgaggag caatccaatg accaacaaga catttaccag attcggtggg ctaagcgcca
     9181 taatcgtcgg tttgctatcc atcctgtacg cgatcttttt tctgatcatc agccgtcaga
     9241 acgagtccct cggcgcgaca ggaagctgga tcattctggc aatgagcggc atcttttcca
     9301 gtgccgcctt cgtggcgttc tatgaacgct tgcagccggt gttcaagggt gccaatatga
     9361 acgcagacaa aatacaggaa aacccgatta cagggatgcg cacaatcgtg attgagacgc
     9421 cggaagagac aggcggccgc cgcgtcgtcg ttgaatacca actgcaacca ggcacgggac
     9481 gcggctatac cgtggcccac atccaccggc gctatgtcga acgcttcgag attctgtcgg
     9541 ggcgcgctgc ttatgtgctc aatggcgtga cgggcgcggc cattgcaggg gaaaccgtca
     9601 ttgtccccat cggaacaagt cacgtccacc cgtggagcgt gggcgatgaa ccgctcgtcg
     9661 ttcggcaaac gaccgaggca gtcgcccccg acgtcaccgg cttgaatagc gcactgatgg
     9721 ccgccgagac cctcatcgac ctgtcccacc agggcaaggt caacgccaat ggccaaccaa
     9781 atccgctgca agcagcggtc atcttccatg atctgctcct gccgcatagc catttggcag
     9841 ggctcccttt tgcggcccag cgagtgatct tcggggttct ttcggccgtg ggtcggctgt
     9901 tgggctatcg cgccgtttac cccgccggca cgattgtggg gacatcagca gtccaatagt
     9961 cggccgctcg tttcgcgatt tccgggcggc gaaaacagat cgatatgccg aacccctctc
    10021 acgggtggag ggaaccggca agaagctcta caatcccctc atgaatacca tccgcacctt
    10081 catcgccgtt cagctaacgg acgaagttag agatgccttg agagaagtag tccggtcatt
    10141 gagcggccgt gttgagcgcg gcgctgttcg ttgggtgcgc ccggaacaga tgcacttgac
    10201 attgcgcttc ctgggcgact ccgataccgg taagctgccc gcagttcagg cggcgatgga
    10261 cgccgtcgcc gcatccaacg cgtccttcga gatgcgcctg ggcgggatcg gttgcttccc
    10321 caacaggaat cggccgcgcg tcatctgggt cggcctgtcg ggagaggaag cacgcttgct
    10381 atccctggtc gtcgctctgg aggaagagct ggcccatctc ggttgggatc ctgagggcaa
    10441 gccctttcgc gcgcatttaa cgttgggccg ggtcaaggac gagcgtggcg cacaagggat
    10501 agaatgggcg gccgatgtgc agcccttggc catccccgtc aaggctatcc acttgatcga
    10561 aagccagctc cgccccaccg gccccgtcta cacgttacgt cattcgagcc ggctggcctt
    10621 gggttgataa tcagtggatc agtaaacagt ggatcagtaa acagtaggtc agtagatcag
    10681 taatcagtag actgacctac tgacctactg acctactgaa cactgatcat cgcacccgca
    10741 ccagatgata ctgtctccgt cccttgcgga ggatgaggaa gcggccgtcg atcgcctgcg
    10801 atagatcggc cgcttgcgtt gcgtcggtca ctcgctcgtt attgagatag atccccccac
    10861 cctgaatagc ccgtcgggcg tcggccttcg aattggccag accgctcgtc acgaggagat
    10921 ccacgaccgc gaggccaccg ctgagcgcct ctttcgccac ctcgttcgac ggcacctcgg
    10981 cgaagatgtc ctcgatgtcg gccgcatcca ggccggtgac atcgccgccg aatagcgcct
    11041 gcgacgcctg ctcggccttc gccaacgccg tctctccgtg aaccatccgt gtaacttcgc
    11101 gggccagcgt ccgttgtgcc tcgcgccgct ccggctgatc gaacagcaga tgctccaatt
    11161 cagcgatgcg accttcgtcg agccaggtga agtattttag ataggtcacg acgtcggcat
    11221 cgtcagtgtt gtaccagaac tgatagaagc gatatggaga tgtgtattcc gggctgagcc
    11281 acacgctggt acccccggcc gttttgccga acttggtgcc atccgcgcgg gtgatcagag
    11341 ggtagacaag cgcgtgggcg cgactgccgc gcacgcgacg aatcagttcc acaccggtca
    11401 ggatattgcc ccactggtca ctgccacccg cctgtagcgc acacttttcg acatcgaaca
    11461 ggtgcaggta gtcataggcc tggagcagca tgtagctgaa ctccgtgaag gagatgccgc
    11521 catcctcgcg ctccagccgc gatttcaccg agtccttggc catcatgtag ttgacggtga
    11581 aatgcttgcc cgtatcacgc agaaaatcaa ccagagacag gcgccgcaac cagtcggcat
    11641 tgttgacgac cttggccgga ttgctcttca cttcgaagtc aagaatgctc tccagctgag
    11701 tcttgatatg gacgacattg gcatcgatct cttccagcga caacaggttg cgctccgtgc
    11761 ttcggccgcc ggggtcgccg atcatgcccg tgccgccgcc ggccagggcg atgggcgaat
    11821 ggccataacg ctgccagcgg gccagctgca tcatcggcac gagatgccca acgtggagcg
    11881 tgtccttcgt cgggtcgaag ccgttgtaaa gcgtgatttt gccggcgctc gtcagctcat
    11941 caagcccctc ggtcttgtcg tagataagtc cgcgccaacg cagctcgtca atgatcgtcg
    12001 tcgccatgcg attactcctg agaacttgtc acatcaatca attttcgtca tggtagtgcc
    12061 tgagcaaatt cagcgccacc acccaaataa caacaaaaaa gacgcggnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnngga tcattgaagt tattgcggcc gtcgcccagg
    12241 atgacgaccg tcgtgcgatt gttcacgctg tcgagccagt tgtgatagaa cgtccccagg
    12301 ctgtgcccaa gatcggtggc gtaatgttgc ggcgcgcgac catagaatct ctccatgacg
    12361 ccatccaccg cttcagccgg ccgattaccc gccatcgtca ggctgatctc ttccatatcg
    12421 ctgtaataac caaaactccg ggtcttggcg acctgatcct gcaactcata ggtgaggcgc
    12481 agcatgaact cggccacaga caacatggag cgagagatgt cgcagatgag cacgaggctg
    12541 ggtttcaact tcttgcgctt gaatttcaac tctatcggca cgccgccata gcgctgattg
    12601 gcgcggatgg tgcccttgga atcgaagcgc ccttccttgc cacgcttgcg tcgcagggca
    12661 gcccggctgc gcaattgcgt caccagccgt tgcacttcct tgcgcaacag attggcctct
    12721 tcctgcgaga gcgcttcgaa cggcttattc atcaggtcgg agccatagag gtcatcgggg
    12781 cgattggcgc gctgctcggc gatccggcgg ccgacgctct gcgccatctg ctcggtcaga
    12841 gcctcgcggt tggcttcgac gacgcccatc agcttctcga tggcttcctg gctcatgccc
    12901 atctccgcca gtcgctccat tagttcgcgc aactggtctt ccagttgagc catgcccatc
    12961 tgttgcaaca ttcgccgggt gacccatcgg gcttcctgct gattgttggc ccattggaca
    13021 cccgcccgct cggccatttg ctccagctct tccttcgtcg gcccctcgcc gctggtcagc
    13081 cagtccatca atgcctgcaa tcggccgctc aacgcctgga gcgcggcctg gagcatctcc
    13141 tgttcatcct cgctcaaatc ctccatcgcg ttttgcatcg gcgggccgcc ggcgctgaag
    13201 tagagcggga atagctcctc gaacacggcg aagtcctcgg actctttgat tagcgtcgtg
    13261 cgcagggatt ggcgaaacag atctttgtcc gtgatgccca gcacctcgat ggcgcgcatg
    13321 gcgtcggccg attcggccat cgacacgcgc accccggcag ctctcatgcc ccgcacgaat
    13381 tcgattatcc gatcgtccat cttccctccg cctccacggg cctatcgtcc gccgggtcgg
    13441 ctgcgaccgc cgccccccat catgcgcttg gccttttgca cgtcggcctc gtacttcaac
    13501 agcacggtca gggtgttgct catggtctct tcgtcaatcg actgggcatt gagcgccacc
    13561 agcgcgcgcg cccagtcgag ggtctcgctg atactcggca ccttgcgcaa atccatgttg
    13621 cgcaattgct gcacagtagc aaccgcctgc cgcgccagat caggcttcag gtcgggcact
    13681 ttcagatgca cgatatcgag ttccgcttcg agtgtgggat acccaacgta gagatagagg
    13741 caccggcgct tgagcgcttc gctcaattcg cgggtgttgt tggaggtcag aagaaccatg
    13801 ggttggtgcc gggcgcgaat cgtacccagt tcgggcacgc tgacctggaa atcgctgagg
    13861 acttcaagca ggaaggcctc gaactccaca tcggcgcggt cgatctcgtc gatgagcagg
    13921 acgaccggct cgtcggacgt cagggctttc agcaacggcc ggggcagcag aaaatgctcc
    13981 gagaagaaga cgtcttcctc ccgcgccagc cggccggcgg cctcggccag gctgctcaca
    14041 tcgcccaata cattatgcaa ggtgtcgcgc aggagctggg tatagagcat ctgcttggcg
    14101 tattcccatt catacagcgc cttgctctcg tccagacctt cataacattg cagacgaacg
    14161 agcggcgcgc ccagcgcggc cgcccaggcc ttggccagtt ccgtcttgcc cacacccgcg
    14221 gggccttcgg ccaggatcgg cttgcacaac gattgagcca gatagatcac cgtggcgatc
    14281 tcatctgacg gaatgtattg ctgcccgctc aatcggcgac ctacatcggc tctgtctttg
    14341 aacatcattc gcatggtctc cgttttt
//
LOCUS       DAOMQG010000047        14220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1839_length_14220_cov_11.736251, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000047 DAOMQG010000000
VERSION     DAOMQG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14220
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_14220_cov_11.736251"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLC06_04460"
     CDS             complement(<1..404)
                     /locus_tag="PLC06_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89728.1"
                     /translation="MESLLAFIPEDRRIALAEGRVLPDRTNGSVLFADISGFTPLTAA
                     LAQELGVQRGAEEVLNQINPVYEAIIAELHRYGGSVMGFAGDSITCWLEGDDGRKGVA
                     CALAMQTAMRPFATVLTPAGTPITLAIKVAVAA"
     gene            complement(515..700)
                     /locus_tag="PLC06_04465"
     CDS             complement(515..700)
                     /locus_tag="PLC06_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRO89729.1"
                     /translation="MNEKRLVRVEEGKMLAGVCTGLARYLGVDVTVVRLLFALLTLFV
                     GGGVLLYLILWLIMPME"
     gene            1088..1327
                     /gene="acpP"
                     /locus_tag="PLC06_04470"
     CDS             1088..1327
                     /gene="acpP"
                     /locus_tag="PLC06_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRO89730.1"
                     /translation="MNDTEERVRAIIIDLLGVDEDQIVTEARFREDLEADSLDLVELI
                     MAFEEEFEGEISDEEAQKITTVGEAVYYINSRMRS"
     gene            complement(1345..1998)
                     /locus_tag="PLC06_04475"
     CDS             complement(1345..1998)
                     /locus_tag="PLC06_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89731.1"
                     /translation="MSGSVRQTALFLGLTAIFVGYFLTWLPGPAAGLQIIGLELGEWI
                     KFLGVGISRNWFYLPPIAMGAIVALLAAMWPNNQWRTWLARLLAIAIALLAFPAIAAI
                     QMEPRSEWLARLVAIGVVTIMAIGGGLIQRDDKAIRWVWLAIVIIAFLGGVLPMGQYF
                     LVRPVVEGMLKQSLGIGIGIWLNLVGALLIGMIALDEMRRGINKKRQSLKQQLPFCL"
     gene            complement(2015..2515)
                     /locus_tag="PLC06_04480"
     CDS             complement(2015..2515)
                     /locus_tag="PLC06_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRO89732.1"
                     /translation="MDGRRVSDSRVTLTQAMGVTEANILGNVHGGVIMKLCDEAGGTA
                     AIKHARRPMVTVAVDSMSFHSPVHIGDLLTVKAEVTWVGRTSLETRLIVTSENLVTGE
                     ITLTNTAYYVYVALDEGGRTVAVPRLICETEEEKRLWEEGADRQALRLERRRHERPIS
                     IEKNDQ"
     gene            complement(2518..3270)
                     /gene="trmD"
                     /locus_tag="PLC06_04485"
     CDS             complement(2518..3270)
                     /gene="trmD"
                     /locus_tag="PLC06_04485"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRO89733.1"
                     /translation="MRIDVLTLFPAMFSAYLDESILQRARKAGLIELNIHNIRDHAEG
                     KHRVTDEPPYGGGGGMVLKPQPIFDAVDAITGEASERRPRTILLSPQGRQFNHDVAVE
                     LASEPWLLFLCGRYEGVDERVREHLMDDEISIGDFVLTGGELAALVVIDALARHVPGV
                     LGDEHAAQRDSYANGLLEGPHYTRPEQYRSWVVPEVLRSGHAGHIERWRREQALRRTW
                     ERRPELLLTADLSEADRHFLMTLAREMTENIR"
     gene            3419..4261
                     /gene="rfbD"
                     /locus_tag="PLC06_04490"
     CDS             3419..4261
                     /gene="rfbD"
                     /locus_tag="PLC06_04490"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRO89734.1"
                     /translation="MHILITGALGQLGRALQNSLAGHSLTLIDLPEVDITSRKDFDHV
                     IGDSRPDLIVHCAAYTNVDGCAKDPILAHKVNALGTQNVALACQSANIPLVHISTNEV
                     FSGDNPGGYDEWMPLSPRNPYGVSKAAAETHVRSLLNRYYIVRTAWLYAPGGRNFIHA
                     IMGRARETGSVRVVTDEIGNPTYVVDLAEAIARLITTGQYGTYHFVNEGSCSRWAFAN
                     EILRLAELTSVTNTPILSSAFNRPSSPPPFGALHNRNGAAIGITLRPWQDALADYLRL
                     ESRD"
     gene            4254..>4945
                     /locus_tag="PLC06_04495"
     CDS             4254..>4945
                     /locus_tag="PLC06_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89735.1"
                     /translation="MTNVSPPRVSVIIPHWNGRQHLHDCLTSLRQQTFANHEVILVDN
                     GSTDGSQGYVRREFPEVVLIDLDKNRGFTGACNAGYAISRGEFVCLLNNDTETDPRWI
                     EVLVNTFERHPRAGIVAGKMLLFDQRDHFHTAGDYYRVNGIPGNRGVWQVDHGQYDRE
                     EPVFSACGGAAAYRRAMVDQIGFLDDAFFFSCEDIDLAWRAHLAGWEVIYAPGAIVYH
                     KLKASGGSGVIG"
     assembly_gap    4946..5045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5176..5943
                     /locus_tag="PLC06_04500"
     CDS             5176..5943
                     /locus_tag="PLC06_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89736.1"
                     /translation="MSAPTPLLSLIIPAYNEGERLPDTLPSVFAFLESQSYAFEIILV
                     NNNSRDNTRKIADEFAATRPYLHVIDEMTQGKGAAVRAGMLAAKGEYLFMADADFSMP
                     VEEIAKFLPPLAGEYDVAIGSREAPGAMRYNEPQYRHFMGRVFNFYVKVLAIPGFEDT
                     QCGFKCFRRHVALDILPYQTIDGWAFDVELLFIALRRGYKIIEVPVHWYYGENSRVSP
                     VRDTVNMIREVLRIRYNGWTGRYDRRTQPDPALPKTR"
     gene            5947..6597
                     /locus_tag="PLC06_04505"
     CDS             5947..6597
                     /locus_tag="PLC06_04505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017712375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRO89737.1"
                     /translation="MATRGPTKSPNPDIERSFAAEFGVVWVAGVDEAGRGALAGPVVA
                     AAVVLPLGNENRLATLAEVRDSKLLSPAARERLYTLICDAAVAYAIGSASAEYIDQHG
                     ILGATRKAMNEAMGSLSPAAEAVLVDGPLRLVSGKLPQRPIVRGDRRSLTIAAASILA
                     KVSRDRYMIELGERSPGYGFERHKGYGTAEHLAALGRLGPCPEHRRSFSPLRASLF"
     gene            complement(6594..7928)
                     /locus_tag="PLC06_04510"
     CDS             complement(6594..7928)
                     /locus_tag="PLC06_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HRO89738.1"
                     /translation="MSSQRPVRVIIAGAAGRDFHDFNTVFRNDESHHVVAFTATQIPN
                     IEGRLYPPALSGPLYPKGIPIHPESELTDLIRKHDVDEVVFSYSDVSHEYVMHLASRV
                     LAAGAGFRLLGPHATMLKSTKPIISVCAVRTGSGKSQTSRKVLDILKDELGYQRVVAV
                     RHPMPYGDLAAQAVQRFAGHADMDAAQCTIEEREEYEPYVDRGAVIFAGVDYEAILRK
                     AEEEADVIIWDGGNNDLPFYKPDFHIVVVDPHRPGHELRYHPGEANLRMADAVIINKI
                     DTADSAGINTVLSNIRAVNPSAIVVRGASPLSVEDPDGIRGKRVLVIEDGPTLTHGEM
                     AYGAGVVAAQQFGAAEIIDPRPYAVRTIADTYRKYPNTGPILPAMGYGREQIADLEET
                     INRAMVDMVIIATPIDLGRLVDIDKPNQRVRYELQEIGRPDLTDLLKERFGR"
     gene            8280..9185
                     /locus_tag="PLC06_04515"
     CDS             8280..9185
                     /locus_tag="PLC06_04515"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HRO89739.1"
                     /translation="MEEQLLAFLSHLQREYRYSENTIAAYRNDLGQFLEYVESHSGAK
                     LEEWAAVSEDDIEAYLSYMKHKDQPYASSTIARKVAAIKSFYNYLTAQGLVDDNPTID
                     IDSPKVKKRLPQTLTIAEVDRLLEAPRSGASPKNLRDVALLNILYETGMRVTEVVSIQ
                     LDDVNLRQALLSSPTRQGEDREIPLEESTKQLLTEYLAEGRPLLAKNNGERALFLNHR
                     GEKLTRQGLWLIIKGYAKQAGLNTEVTPHTLRHSFAVHRLSKGSSLEDIRHLLGHANI
                     STTQIYTQMEHSEEEKDISNAASAA"
     gene            9204..10058
                     /locus_tag="PLC06_04520"
     CDS             9204..10058
                     /locus_tag="PLC06_04520"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRO89740.1"
                     /translation="MDHFITSTEIKAAADYVRAHTRYQPTVGLVLGSGLNSLADTIEF
                     PDIIPTGEIPHWPVSTVPGHVGRLIIGNLEGQSVLVLQGRTHFYEGYSMDRVTLPIRV
                     MAELGIDTFIVTNASGGLNPAYEPGDLMIIRDHLNFPGMAGNNPLRGPNNESLGPRFP
                     DMTTAYDARLRSLAHRVGEEAGVTLREGVYAYVAGPSFETPAELRFLQFAGGDAVGMS
                     TAPEVVVARHAGIRVLGISTVANMALPDPPPGLTLNHEEVLEMGLRAVPRLITVVRGV
                     LGRLDEVR"
     gene            10120..10908
                     /locus_tag="PLC06_04525"
     CDS             10120..10908
                     /locus_tag="PLC06_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89741.1"
                     /translation="MDRFYLFLIRNDVWIYIVSVLGLFWYLTEFIRAMRTLRRAMFNL
                     EKETATAARNHALGFILFFSAVIGLVFYVNRNIAPNLPDTVIVDASPTPDIFATPLSP
                     PTPISTPLIEGALNPAPVLAPTVTLPSAPGVDASTTITDTVSADGTTAPGLTPTPFEA
                     CIPELTIAEPLSGSVVFQRATFRGTANTGELHQYVIELNGPQTAGVWAPISQDPVNQP
                     IVNGDLGQADLTQWQPGPYRVRMRALDATGNELGQCIIQITLDN"
     gene            complement(10912..11469)
                     /gene="rsmD"
                     /locus_tag="PLC06_04530"
     CDS             complement(10912..11469)
                     /gene="rsmD"
                     /locus_tag="PLC06_04530"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRO89742.1"
                     /translation="MAARVISGKAKGRRLKLVPGDTTRPIMDRVKESLFNILGDIDGT
                     RWLDMYAGTGQVGIEALSRGAAEVVFVDKARAAVQTVRDNLTHTQLTVGTTVVQGDAL
                     AFLRDMGGPPFDVIYIAPPQYLEMWIGALQAIDGNTDRYLAADAQVIVQIDPREYREL
                     ALSRLSLADQRRYGNTLLCFYRPVV"
     gene            complement(11472..>11885)
                     /locus_tag="PLC06_04535"
     CDS             complement(11472..>11885)
                     /locus_tag="PLC06_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89743.1"
                     /translation="DARELYANFGDQIALLGYTGIGETAVPGSKVEFTVYWKALRELE
                     ISYQSFVHVLDANGNLVAQSDKLNPGEFPTNRWPLDKYVPDTHRIVLPHDLPKGEYTV
                     VTGLWVMGEGWRLPVFDENGETIGDNVELFRFRVE"
     assembly_gap    11886..11985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11986..13424)
                     /locus_tag="PLC06_04540"
     CDS             complement(<11986..13424)
                     /locus_tag="PLC06_04540"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HRO89744.1"
                     /translation="MINRLRRGFDPGYLVVLLICLLAVWPLISRASLPEGTDAELHIF
                     RLHELSYLVRGGEFYPRWAPNFYHGYGYPIFNYYAPLAYYLALPLELLPSVDAVLATK
                     AVLVMGMLLGGIGIYGFTRDNWGRGGGYVAAALLAYSPYLQYIDPHVRGVVPEMLSFG
                     LFPLALWALDRLWRRPSAGSWLASVFLVAAVILSHNLMGLFLFGLLTAWVSWQAILTY
                     SARRKTRESESTHSSWTIIGRLMTALALGLGLAAFFWLPVFLERNAVTLNTLIGAGDN
                     YDFRTHFLPLIELLGPSISPDWGATQSPFRFNLGVAQWIGGVLGLIMLALGRVKQRSQ
                     LLFFALAAGATILLMTSASQSIWETIPFLPYFQFPWRLLGAAAALLAVLAAGGVEALA
                     GSLDQADARVAPSRSSWVYGIVVGATILLALPLSQPAPWEPFGEVNTLRMSLIENSGR
                     WLGTTSTADYVPATVITLPQRRDSVVGPIA"
     gene            complement(13519..>14220)
                     /gene="sucD"
                     /locus_tag="PLC06_04545"
     CDS             complement(13519..>14220)
                     /gene="sucD"
                     /locus_tag="PLC06_04545"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRO89745.1"
                     /translation="IFDSVRGAAEATGGNTSIIFVPAKFAPEAMYEAADAGLPLVICL
                     TEHIPVLDMIKVRSYLDQRQTRLIGPNCPGLITPGEAKVGIIPGSIVTPGTVGLVSRS
                     GTLTYEVVYALTQRGIGQSTCVGIGGDPIKGMDFIDVLKLFEADPNTSEVIMIGEIGG
                     NAEEQAAAYIAENMTKRVTSFIAGRSAPPGRRMGHAGAIVEGKSGTAEGKIAALEAAG
                     VQVANNPDHIVEMIA"
BASE COUNT         3134 a         4223 c         3827 g         2836 t
ORIGIN      
        1 gcggccacag cgaccttaat cgccagagtg atcggcgtgc cggccggggt caggactgtc
       61 gcgaacggcc gcatcgcggt ctgcatagcc agcgcgcagg cgaccccttt tcggccgtcg
      121 tcaccctcaa gccaacaggt tatcgaatct ccggcaaaac ccatcacact gccgccatag
      181 cgatgaagct cggcaatgat ggcttcgtag acggggttga tctgattgag aacttcttca
      241 gcgcctcgtt gaacgcctaa ttcctgggcc agagcggccg tcagcggtgt gaagcccgaa
      301 atatcggcga atagcacgga gccgttggtg cggtcgggca agacacgtcc ctcggcgagc
      361 gcgatgcgcc ggtcttcggg aataaatgca agaagtgact ccataatgtt cgccggaaaa
      421 aataaggacg gtggtaaaat ttcttaccac cgtcccaccc catacttagt ataggcacaa
      481 tcccaccgcg cggcaatcga atccgccctg aggtttattc cattggcata atcagccata
      541 aaatgagata cagtaataca ccgccgccga caaaaagtgt cagcaaggca aagagcagac
      601 gcacaaccgt cacgtccacg cccagatatc gagccaaacc agtgcaaacc ccggcgagca
      661 ttttaccttc ttcaacacgg acaagtcgtt tttcgttcat ggttatctcc taactgtagt
      721 tcttctgcga tcaataaaat gagtatctat ctgtctatca atgtgaccgg cgatattttc
      781 tatatctttc tacgttgccc aaactcgggg agttgcgaaa gatccctgat tcggacatgg
      841 aataacgcaa ctaaacccaa atgtccgtgt ttaatttgac agattgaagc gaagttaacg
      901 ccctgcctgg gggcttgaca ttctggctgt aagtcctaca attccagcct gttacgaatg
      961 ggtttactcg ggtttgcagc gaatttattt ccaacagtct acccgatttt tgttaaatag
     1021 ataaataaca aggcgaccgc cgcgagcggc gcaacaagaa ataatgacct tgaaggagag
     1081 gcagtcaatg aatgacactg aagaacgggt tcgcgcgata atcatcgatt tgctcggcgt
     1141 ggacgaggac caaattgtaa ccgaagcgcg cttccgcgaa gaccttgagg ccgattcgct
     1201 cgacctcgtt gagctaatca tggcctttga ggaagaattt gaaggcgaga tctcggacga
     1261 ggaagctcaa aagatcacga ctgtgggtga agcggtctat tacatcaaca gccgcatgcg
     1321 gtcctaagtc tctccggtca ccatctataa acaaaaaggc agttgctgtt tcagcgactg
     1381 ccttttttta tttatccctc tgcgcatctc gtcaagcgcg atcatgccaa tgagcaacgc
     1441 tccaaccaag tttagccaga tgccgatccc aatgccgagg gattgtttta gcataccttc
     1501 tacgaccggc cgcaccagaa aatactgacc catcggcaaa accccgccca gaaaagcaat
     1561 tattacgata gccaaccaca cccatcgtat ggccttatcg tcacgctgaa tcagaccccc
     1621 gccgatcgcc ataatggtca cgacaccaat cgcgaccagg cgagccagcc attcgctccg
     1681 tggttccatc tgaatagccg caatagccgg aaaggccagc aaagcgatcg cgatcgccag
     1741 taaacgggcc agccaggtac gccactgatt attcggccac atggccgcca gcagggccac
     1801 aatcgcgccc atcgcgatgg gtggcagata gaaccagtta cggcttatgc caactcccag
     1861 gaatttaatc cactcgccca gctcaaggcc aataatttgc aagcccgcag ccggaccggg
     1921 gagccacgtc aggaaatagc ccacaaatat ggccgtcagc cccaggaata aggcagtttg
     1981 tcgaacagag ccagacatag cgctaaatgt gcccctattg atcgttcttc tcaattgaga
     2041 tcggccgctc gtgacgacga cgttccaggc gcagcgcttg cctgtcggct ccttcctccc
     2101 acagtcgctt ttcctcctcc gtctcgcaga taaggcgcgg caccgccacc gtacggccac
     2161 cctcatccag ggcgacatag acatagtagg ccgtgttggt gagcgtgatc tcgccggtga
     2221 cgagattttc ggacgtcacg atcagccggg tctcaagcga ggtgcggcca acccaggtca
     2281 cctcagcctt tacggtcagg agatcgccga tatgaaccgg ggagtggaag ctcatggaat
     2341 cgactgccac cgtcaccatc ggccgccgcg catgcttgat ggcggccgtg cctcccgcct
     2401 cgtcgcacaa cttcatgata acaccaccat ggacattgcc gaggatgttt gcctcggtta
     2461 cgcccatcgc ctgggtcaat gtaactcgcg aatcgcttac ccttctaccg tccatattca
     2521 ccgtatgttt tccgtcatct cgcgcgcgag agtcatcagg aaatgtcgat cggcctcgct
     2581 caggtctgcc gtcaataaaa gctccggtcg tcgctcccag gttcggcgaa gagcctgctc
     2641 gcgtcgccag cgttcgatat gtccagcgtg gcccgatcga agcacctcag gcacgaccca
     2701 cgaacggtac tgttccggcc gcgtgtagtg gggcccctca agaagaccgt tggcatagct
     2761 atcccgctgg gctgcatgct catcgcccag cacacccggc acatgacgag ccagcgcgtc
     2821 gatcaccacc agtgccgcca actcacctcc ggtgaggacg aaatcgccga tagatatctc
     2881 atcatccatc aggtgttcgc gcacgcgctc gtcgacgcct tcatagcggc cgcaaagaaa
     2941 tagcagccac ggctcgcttg ccagttcaac cgcgacatcg tggttgaact gccttccctg
     3001 aggggaaagc aaaatagttc ggggacggcg ttcactcgct tctccggtga tcgcatcaac
     3061 ggcgtcgaag atcggctgcg gcttaagcac catcccgcct ccaccgccat aaggcggttc
     3121 atcggtcacg cgatgtttgc cctccgcatg gtcccgtatg ttgtggatgt tcaactcgat
     3181 caaccccgct tttcgcgcac gttgcagaat gctctcgtcc aggtaggcgg agaacatggc
     3241 ggggaaaagt gtcaggacgt cgattcgcat taatatcact cgggcaagct ataccaaggt
     3301 ccaagtattg agtttaagtc acttcctgtc aagcatgcga cgacatgccg acggcttcac
     3361 atcgacctgt cgacatgtca ttgacaactt gcggcgggca aaccacaatt atgggtgtat
     3421 gcatattctc atcaccggcg ccctgggaca attggggcga gcattacaga acagtctggc
     3481 gggccattct ctaaccctga tcgatctgcc ggaagtcgac atcaccagtc gcaaggattt
     3541 cgatcatgtt attggtgatt cacgcccgga tttgatcgtc cattgcgcgg cctacacgaa
     3601 tgtcgatgga tgcgccaaag acccgattct ggcgcacaag gtcaacgcgc tgggaacgca
     3661 aaacgtggcc ctcgcctgtc agtcggccaa tatcccgctg gttcatatca gcaccaatga
     3721 ggttttctcg ggcgacaacc ctggcgggta cgatgaatgg atgccgctct ccccgcgtaa
     3781 tccctatggc gtgtcgaagg ccgcggccga aacgcacgtg cgctctctgc tcaaccgcta
     3841 ctacatcgtc cgcacggcct ggctctacgc tccgggcggc cgcaatttca tccatgccat
     3901 catggggcgc gcacgcgaaa ccggatctgt gcgcgttgtg accgacgaaa tcggtaatcc
     3961 gacctatgtt gtcgatctgg ccgaggctat cgctcgattg atcaccaccg ggcagtatgg
     4021 cacatatcat ttcgtcaatg aaggttcttg ctcgcgctgg gccttcgcca acgaaattct
     4081 gcgactggcc gaactcacga gcgtgaccaa tacgcccatc ctcagcagcg ccttcaaccg
     4141 cccgtccagc ccaccgccat tcggcgcgct gcacaaccgc aacggcgcgg cgatcggcat
     4201 taccttgcgg ccgtggcagg atgcgctggc ggattatctg cgcctggaat cacgtgacta
     4261 acgttagccc cccacgcgtg tcggtcatta ttccacattg gaacggccgc caacatctgc
     4321 atgactgtct gacctcgctg cgacagcaaa cattcgccaa tcatgaggtc atcctggtcg
     4381 acaacggctc aaccgacggt tcgcagggat atgtgcgcag agaattccct gaagtcgtcc
     4441 tcatcgacct agacaaaaat cgcggcttta ccggagcgtg caatgccggc tatgcgatca
     4501 gccgggggga gtttgtgtgt ctgctaaaca atgacactga aaccgacccg cggtggatcg
     4561 aagttctcgt caataccttt gaacgacacc ctcgggcggg catcgtcgcc ggcaaaatgc
     4621 tgctgttcga ccaacgcgat catttccaca cggccggcga ttactatcgg gtaaacggca
     4681 tccccggcaa tcgcggggtc tggcaggttg atcatggcca atatgatcgg gaggagccgg
     4741 tattcagcgc gtgcggtggc gcggccgcct atcgtcgggc tatggtggat cagatcgggt
     4801 tcctcgacga tgccttcttc ttctcgtgcg aggatatcga cctggcctgg cgagcacatc
     4861 tggccggatg ggaagtcatc tacgcgcctg gggcaatcgt ctatcacaag ttgaaggctt
     4921 ccggcgggag cggcgtcatc ggcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnngatga ccgcaccctg gagtcacgtc tgagccgggt tgacgaggtt ttatagggca
     5101 gatacacttg ttttttcgcc ttattcggag cgaacagttc aaattcacgt ccacggccgc
     5161 aaggcgattc gcagcatgag cgcaccaaca cccctcctga gtctgatcat ccccgcctat
     5221 aatgagggag aacgtttacc tgacacgcta ccctcggtat ttgctttcct cgaatcccag
     5281 tcatacgcct tcgagatcat ccttgtcaac aacaacagcc gggacaacac acgaaaaatc
     5341 gctgatgaat ttgctgctac tcgcccctac ctccacgtca tcgacgagat gacgcaaggc
     5401 aaaggtgccg cggtgcgggc agggatgctg gccgccaaag gtgaatatct cttcatggct
     5461 gacgccgatt tctcgatgcc ggtagaagag atcgccaagt ttctgccgcc gctcgccggt
     5521 gaatatgacg ttgccatcgg cagtcgcgaa gctcctggcg caatgcgcta taacgagcct
     5581 cagtatcgcc acttcatggg gcgggtcttc aacttctatg tgaaagtgct ggccattccc
     5641 ggctttgagg acacccaatg cggtttcaaa tgttttcgac gacacgtggc tctggatatc
     5701 ctgccttacc aaacaattga cggctgggcg ttcgacgtgg aactgctctt catcgcgctg
     5761 cgacgcggct acaagatcat cgaagtgccc gtccattggt actacggcga gaacagccgg
     5821 gtgagccccg tgcgcgatac ggtcaacatg atccgcgagg tcttgcgcat ccgctataat
     5881 ggctggaccg gtcgctacga tcgccgtacc caacctgatc cggctctccc caagactcga
     5941 taatcgatgg ccacccgagg ccccacgaaa tcccccaacc cggacatcga gcgctccttc
     6001 gccgcggaat tcggcgtagt gtgggtcgcc ggagtcgatg aggccgggcg cggggcgctg
     6061 gccgggcctg tggtagctgc cgctgtggtg ttgcccctcg gcaatgaaaa taggctggcc
     6121 acattggcgg aagtgcgaga ttccaagctg ctctcccctg cagcccgtga acgcctctat
     6181 acgttgatct gcgacgctgc cgtggcttac gcgattggtt cggcttcggc cgaatacatc
     6241 gaccaacacg gcatcctggg agccacacgc aaggccatga atgaggccat gggcagtttg
     6301 tcaccggcgg ccgaggctgt gttggtcgac ggcccactgc ggctggtgag cggcaaactg
     6361 ccgcaacgcc ccatcgtgcg cggcgacagg cgcagcctca cgattgccgc ggcctcaatt
     6421 ctggccaagg tgagccgcga ccgatatatg atcgagttag gagagcgttc acccggttat
     6481 ggctttgagc gccacaaggg gtatgggacg gccgagcacc tggcggcgct cggccgattg
     6541 ggtccctgcc ccgaacatcg gcgcagcttc tcacctctgc gggcatcctt gttctagcgg
     6601 ccgaacctct ccttgagcag gtcagtcaga tcgggccggc caatctcctg caattcatag
     6661 cgaacgcgct ggttgggctt gtcaatgtcg accagccgcc ccagatcgat cggcgtggca
     6721 atgatgacca tatcaaccat cgcccgattg atcgtctcct caagatcggc tatctgctcg
     6781 cggccgtacc ccatggcagg caaaattggc cccgtgttcg gatacttgcg ataagtatcg
     6841 gcaatagtac gcactgcgta aggtcgtgga tcgatgatct cagccgcgcc aaactgctgg
     6901 gccgccacta ccccggcacc ataagccatt tcgccatggg tcagcgtagg gccgtcctcg
     6961 atcaccagca cgcgcttgcc gcggatgccg tccgggtctt ctaccgacaa gggggatgcg
     7021 ccgcgaacaa cgatagccga cggattgacg gcgcggatat tgctcaacac ggtattgatg
     7081 cccgccgagt cggccgtatc aattttgttg atgatcacag catcggccat gcgcaggttc
     7141 gcctcaccgg gatgatagcg cagttcgtgg cctggccggt gcgggtcaac caccacaatg
     7201 tggaaatcgg gcttatagaa gggcagatcg ttattcccgc catcccagat gatcacatcc
     7261 gcttcctctt cagctttgcg cagaatcgct tcgtaatcca cgccggcaaa aatcactgcg
     7321 ccgcgatcga cgtatggctc gtattcttcg cgctcctcaa tcgtgcattg cgccgcgtcc
     7381 atatcggcat ggccggcaaa acgctggacc gcctgtgccg ccagatcgcc atagggcatg
     7441 ggatggcgaa cagccacgac gcgctgatag cccagttcat ccttcaggat gtccagcact
     7501 ttgcgcgagg tctggctctt gccactgccg gtgcgaacgg cacaaacgga gataatcggc
     7561 ttggtcgact tcagcatcgt ggcgtgtggg cctaacaggc gaaaccccgc cccggcggca
     7621 agcactcttg aggcaagatg catgacatat tcatgcgaca cgtctgaata ggagaagacg
     7681 acttcatcca catcatgctt gcgaatcaaa tccgtcagtt cgctttccgg gtgaatgggt
     7741 atacctttcg gatacaatgg ccccgacagc gccggcggat agaggcgtcc ttcaatgttg
     7801 ggtatttgcg tagccgtgaa ggcgacaacg tgatggctct cgtcattcct gaataccgta
     7861 ttaaagtcgt ggaaatctcg gccggcggct ccggctatga tgacacgcac aggtcgttgg
     7921 gaactcatgg aaaaggctct cctttgaaat atggaattaa gctgtagctt tggatggaaa
     7981 gttgggttga cgcacgtagg tggcgccaat tggcagtgaa atcaccccta atgctacccg
     8041 aaaaatcata aacaggcatt tgatattatg aaaagtagtg ttataatttc tggaagttgc
     8101 atgacataat ctgtaaggcg ctgcgcaggg ttcagtaagc tccctggcca tactgatgaa
     8161 attgcaacag gcaattcgag aggagatgta ttgtaactgg ctgcccgcca cggcccagct
     8221 ccctccagtt cgaaagcaat gataactcta ttcagctacc gatcagggag tagaatgcca
     8281 tggaagagca attgcttgcc ttcttgtcgc acctgcagcg agagtacagg tattcggaaa
     8341 acacaatagc tgcgtatcgt aatgatctcg gtcagtttct ggaatacgtg gaaagccact
     8401 ccggggcgaa actggaagaa tgggccgccg tttccgagga tgacatcgag gcttatctga
     8461 gctacatgaa gcataaggac cagccttatg cttcttcgac tatcgctcgc aaagtcgccg
     8521 ccatcaagtc tttttataac tacctgacgg cgcaagggct ggtcgacgac aacccaacca
     8581 tcgacatcga ctccccaaaa gtcaagaaga gactgcccca aacgctgacc attgccgagg
     8641 tcgatcggct cctggaggcc cctcgcagcg gcgcttcgcc caagaacctg agagatgttg
     8701 ctcttctgaa tatcctgtat gaaaccggca tgcgcgttac cgaggtcgta tctatacaac
     8761 tcgacgacgt gaatctccgg caggctttgc tttcatcacc tacaaggcag ggtgaggacc
     8821 gtgaaattcc cctggaagag tcgaccaaac agctcctgac cgaatacctg gccgagggcc
     8881 gaccgctcct ggccaaaaat aacggggagc gcgccctgtt cctgaaccat cgcggcgaga
     8941 agctgacacg gcaagggcta tggctaatca ttaagggata cgccaaacaa gcaggcctca
     9001 acacggaagt gacgccccac accttgcgtc acagcttcgc cgtgcatcgc ctctccaagg
     9061 ggtcaagtct ggaggatatc cggcacttgc tgggccacgc caatatatcc accacacaga
     9121 tttacaccca aatggagcat tccgaagaag agaaagatat ttccaacgcg gcatcggccg
     9181 cctgagcagg gacggttttt catatggatc atttcatcac ttctaccgag atcaaagccg
     9241 cggccgatta cgtccgtgcc cacacccgat atcagcctac cgttgggctt gttcttggct
     9301 ccggcctgaa ttcgctggct gatacgatcg aattccccga catcatcccc accggcgaga
     9361 tcccgcattg gccggtatcc accgtccccg gccacgtcgg gcggttgatc atcggcaatc
     9421 tggaagggca gtcggtcctc gtcctgcagg gaaggaccca tttctatgag ggatactcga
     9481 tggatcgggt caccctgccc atccgtgtca tggcggagtt gggcatcgac acgtttattg
     9541 tgaccaacgc ctcaggcgga ttgaaccctg cctacgagcc gggcgatcta atgatcatcc
     9601 gcgaccatct caacttcccc ggcatggccg gcaacaatcc gctgcgaggc ccgaacaacg
     9661 aatcactcgg cccccggttc cccgacatga cgacggccta tgacgcccgg ctccgctcgc
     9721 tggcgcatcg ggtcggtgag gaggccggcg tcacgctgcg cgaaggagtt tatgcctatg
     9781 tggccggccc cagcttcgag actccggctg aattgcgatt cctgcaattc gccggtggcg
     9841 atgcggtggg catgtccacg gccccggaag ttgtcgtcgc tcgccatgcc gggattcgcg
     9901 tgctgggaat ctcaaccgtg gccaacatgg ccctgcctga tccgccgccg ggtctgaccc
     9961 tgaatcacga agaggtgctg gagatgggat tacgcgccgt gccgcgcctg atcaccgtcg
    10021 tgcgtggtgt gttgggccgg ttggatgaag tgcgctgatt ccgcgaaaat attggacttc
    10081 tcgcattctc cccggcgctg agccacgtca cataccacga tggatcgctt ttacttgttc
    10141 ctgatacgca acgatgtctg gatctacatc gtcagcgtcc ttggcctctt ctggtacctg
    10201 accgaattca tccgggccat gcgcaccctg cgtcgggcca tgttcaatct ggaaaaagag
    10261 acggccacgg cggcgcgcaa tcacgctctg gggtttatcc tctttttttc ggccgtcatc
    10321 ggtcttgtat tctacgtcaa cagaaatatc gccccgaatc tgcccgatac tgtcatagtc
    10381 gatgccagcc cgacgccgga cattttcgcc accccattaa gcccaccgac gccaatcagc
    10441 acaccgttga tcgaaggtgc gcttaaccct gctccagtgc tggcgccgac ggtgacgctg
    10501 ccctcagcac cgggagtgga cgcctccacg acgatcacgg atacggtatc ggccgatggc
    10561 acaacggcgc ccgggttgac ccctactccc ttcgaagcct gcatccctga actgacaatc
    10621 gcggaaccat tgagcggctc agtcgttttc cagcgggcaa cattccgcgg cacggccaat
    10681 accggcgaat tgcatcaata cgtcatcgaa ttaaacggac cgcaaacggc gggggtatgg
    10741 gcgccgatca gccaggatcc ggtcaaccag ccaatcgtca acggcgattt gggtcaggcc
    10801 gatctgactc aatggcagcc cgggccttat cgggtacgca tgcgggcgct cgatgccacc
    10861 ggcaacgaac tcggccaatg cattatccag atcacgctgg ataactaacg tttagaccac
    10921 aggccggtag aaacagagga gtgtgttgcc gtagcgccgc tgatcggcga gtgagagccg
    10981 gctcaacgcc aattcacggt actcgcgcgg atcaatctgg acgatgacct gggcatccgc
    11041 ggccagatag cgatctgtat tcccgtcaat ggcttgcagc gcgccaatcc acatttccag
    11101 atattgcggc ggggcgatat agatgacatc aaaaggcggc ccccccatgt cgcgcaaaaa
    11161 ggccagcgca tccccttgaa cgacagtggt accaacggtc aattgcgtat gggtcagatt
    11221 atcacggaca gtctgcacgg ccgcgcgcgc tttatccacg aagacgactt cggccgcacc
    11281 gcgactcagc gcttcaatcc cgacctgccc tgtccccgcg tacatgtcca accagcgcgt
    11341 gccgtcgata tcacccagga tgttgaacag gctctccttg acgcgatcca tgatcggccg
    11401 cgtcgtgtcc cccggcacca gtttgagccg tctgcctttt gccttcccgc tgatcactcg
    11461 cgccgccata cttactccac cctgaacctg aatagttcga cattatcgcc tattgtctcg
    11521 ccattttcat caaataccgg caagcgccag ccctcgccca tcacccaaag gccggttacg
    11581 acggtatatt ctcctttggg taagtcatgt ggcagaacga tccggtgagt gtcggggacg
    11641 tatttatcca aaggccaacg gtttgttgga aattcgcccg ggttcagctt atcggattgg
    11701 gccaccagat tgccgtttgc atccagcacg tggacaaatg actggtagct aatctccaac
    11761 tcccgcaagg ctttccaata gacggtaaat tccaccttgc tgcctggaac ggccgtctcg
    11821 ccgatcccgg tataacccag caaagcgatc tgatcgccaa aattggcgta cagttcgcga
    11881 gcatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcaat cggcccaacg
    12001 accgaatcac gccgctgcgg cagggtgatc accgtcgcgg ggacataatc ggccgtcgag
    12061 gtagtgccca gccaacggcc gctattttcg atcaatgaca ttcgcaacgt gttgacctcg
    12121 ccgaaaggct cccacggcgc gggctgacta agcggcaagg caagcaagat cgtcgcgccg
    12181 acgactatcc catagaccca ggatgagcga gacggggcga cgcgcgcgtc agcctgatca
    12241 agactcccgg caagcgcttc cacaccaccg gcggccaata cggccagcaa tgccgccgcc
    12301 gcgcccaaca ggcgccatgg aaactggaaa tacggcagaa acgggatcgt ttcccatatc
    12361 gactgagagg ctgaagtcat caggagaata gtagctccgg cagcaagagc aaagaagagc
    12421 aattgactgc gctgctttac ccgaccaagc gccagcatga tcaagcccaa tacccccccg
    12481 atccattggg ccacgcccag attgaacctg aacggagact gcgtcgcgcc ccaatcgggt
    12541 gagattgagg gaccaagcag ttcgatgagg ggcaggaagt gggtgcgaaa atcgtagtta
    12601 tcgcccgcgc cgatgagcgt gttgagagtg acggcgttgc gttccaaaaa cacgggcagc
    12661 caaaagaaag cggccaaccc caggccgagg gccagggcag tcatcagacg gccgattatc
    12721 gtccatgagg aatgagttga ttcgctttcc ctcgtcttcc gccttgccga ataggtcagg
    12781 atggcttgcc agctaaccca ggcagtcaga aggccaaaga ggaacaggcc catcagatta
    12841 tggctcagga tgacggcggc aacgaggaaa acagatgcga gccacgaacc ggcgcttggc
    12901 cgtcgccaca gccgatccaa tgcccatagt gcaagaggaa acagtccgaa gctgagcatc
    12961 tccggcacaa cgccgcgaac gtgtggatcg atatattgca agtaaggcga ataggccagc
    13021 aacgcggcgg ctacatatcc cccgccgcgt ccccaattgt cgcgggtgaa gccatagata
    13081 cctattccgc ccaacaacat tcccatcacc agaacggcct tagtggccaa cactgcatcg
    13141 acgctcggca gcagctccag cggcaaggcg aggtaataag ccagcggcgc gtaataatta
    13201 aaaatgggat agccgtaacc gtggtagaaa ttgggcgccc aacggggata gaactcgccg
    13261 ccacgcacca gataactcag ttcatggagc cggaagatgt ggagttccgc gtcggtgccc
    13321 tccggcaaac tcgcgcgact aatgagcggc cataccgcca gcaggcaaat cagcaggacg
    13381 acgagatatc cgggatcgaa cccgcgccgc aatcggttta tcacagagtg aaataatcca
    13441 cagaatacgc cgattgcaca caattctcat cttgcatgca aatcggcgta tcctgtcggt
    13501 ttaatgggga gaggtcgatc aggcaatcat ctcaacgatg tggtcggggt tgttggctac
    13561 ctgcacgccg gccgcctcaa gcgcggcgat cttgccttcg gccgtgccgc tcttgccctc
    13621 gacaatcgct ccggcgtggc ccatgcgacg gcctggagga gcactgcgcc cggcgatgaa
    13681 cgacgttacc cgcttggtca tattctcagc gatgtaggcc gcagcctgct cctcagcgtt
    13741 gccgccgatc tcgccaatca tgatcacctc ggacgtgttg gggtccgcct caaacagctt
    13801 gagcacatcg atgaaatcca tacccttgat cggatcaccg ccgatgccca cacaggtgga
    13861 ctgaccgatg ccgcgttgcg tgagggcata aacgacctca taggtgagcg tgccgctgcg
    13921 cgacacgagg ccgacagtgc ccggcgtcac gatggagccg gggatgatac cgaccttggc
    13981 ttctcccggc gtaatcagcc cggggcagtt cggcccgatc aaccgggttt gccgttgatc
    14041 cagataggaa cgaaccttga tcatgtccag caccgggata tgctcagtga ggcagatgac
    14101 cagcggcaag cctgcgtcgg ccgcctcata catggcttcg ggggcgaact tggcgggcac
    14161 gaagatgatg gacgtgttcc caccggtcgc ttcggccgct ccgcggacgg aatcaaagat
//
LOCUS       DAOMQG010000048        14211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1840_length_14211_cov_12.221178, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000048 DAOMQG010000000
VERSION     DAOMQG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_14211_cov_12.221178"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1249
                     /locus_tag="PLC06_04550"
     CDS             47..1249
                     /locus_tag="PLC06_04550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRO89746.1"
                     /translation="MSEVETIDVLIVGSGPSGTSTALHLIKHDPSWAKRIVIVDKAVH
                     PREKLCGGGITHLGRNVLSDLGLTLEPRHFDVTEARLVYHDKSYSFFSDPVFTIVRRD
                     EFDHWLVKTAEKLGVLVRQGEAVKEITPHADYVEVVTELATFRAKVVVAADGSRSYVR
                     RKLKWAGDEPGTNEPHVARLIEVLTPEDPATTPEFRDGVAVFDFTPMDDGLQGYYWDF
                     PSFIKGKAYMNRGVFDSRARPEKPKADLKEILAGELAERGRQLDDYELKGHPIHWWNA
                     RGKFARPRVLLVGDAAGADPLMGEGISFALGYGDPAAATIIDAFAHDNFSFDSYRERL
                     AAHPLFRQLDRRVRLARYAYWLNRPWLAGLGWRVMRLVMRFTSWSDRSHVPIREPEFS
                     LIEASKTV"
     gene            complement(1256..1966)
                     /locus_tag="PLC06_04555"
     CDS             complement(1256..1966)
                     /locus_tag="PLC06_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89747.1"
                     /translation="MLESPPETQRILVVDDNTYTLRIVQHTLEQGGYEVSTAVSGPEA
                     LKLINRHGLPHLAIVDLHMPDMSGFEFCRSIHEFSDVPVIMLTAANTEETIVEGLEEH
                     AEDYMVKPFNPPELIARVRRVLGRMEDYTYTLESTTRIDDRLLINFPLREATVDGQAV
                     SLTPTETKLLYILVRNGGRIVTTDFLLNRIWPLQEAREDRLHVHVHRLRGKIEQDPNN
                     PVYILAERGIGYTFQNLG"
     gene            2250..2606
                     /locus_tag="PLC06_04560"
     CDS             2250..2606
                     /locus_tag="PLC06_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRO89748.1"
                     /translation="MQADWQYIAVFVALSPIFPLAPVVLNRLLGPHRPNPIKQQTYEC
                     GVETVGDTWIQFKVQYYIYALVFVVFDIEAVFLFPIAAAYGQLDLFAIIATVLFIFLL
                     ADGLIYAWSRGVLEWV"
     gene            2692..4026
                     /gene="nuoH"
                     /locus_tag="PLC06_04565"
     CDS             2692..4026
                     /gene="nuoH"
                     /locus_tag="PLC06_04565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRO89749.1"
                     /translation="MNMIIDLLKSLISLSLGDFIRRNFGTGPGTELLIDIGGVVILST
                     FCLLIVIFLIWLTRKLVARMQDRIGPNRVGGRYGLLQTVADVVKLLSKELIHPSGADW
                     LSYNAAPILTVMAALLVWAVMPFAPGVIGVDLNIGVFYIMAISSISVVVLLLAGWGSN
                     NKYALLGAFRAVAQMVSYEVPMMLALLVPVILARTMSTKGIIDAQNLPFILFAPVSAL
                     IFYISTLAETGRTPFDLLEAESEIVAGFHIEYGGMKFGMFFLAEFVNTLFWSGMFVTL
                     YLGGYRIFGLENWVGGDGYPYGRLIGLIAFFAKTFFMYFVYDWIRGTLPRVRVDQILN
                     FNWKFMVPLSLVLIFFVALVDKFIPDTAGTVTRMAIHLTLNLVIAAATLEFLRRRGRQ
                     FRQEMSGGDGPGGRETLYPDDHGHGAAHEAPAHSDHGDMPLGGREPIAVPLH"
     gene            4053..4604
                     /locus_tag="PLC06_04570"
     CDS             4053..4604
                     /locus_tag="PLC06_04570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRO89750.1"
                     /translation="MSQQIIFFILAAITLTSAIAVVTMRNLFHAALALMVSFLGVAGI
                     YVLLEAGFMGMAQLLVYIGAISILIIFAVMMTRRMMQTRETPFNAQTLAALVGTLITM
                     AILFVVIGRLYPLAPTTEIPLGAAPQVDPAIIGSSIDQLGRAFVGANAYVLPFEVASV
                     LLLAALVGSIIIAWPRSGEEDNL"
     gene            4601..4909
                     /gene="nuoK"
                     /locus_tag="PLC06_04575"
     CDS             4601..4909
                     /gene="nuoK"
                     /locus_tag="PLC06_04575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRO89751.1"
                     /translation="MIPLSWYLLLSAALFSVGLFGVLARRNAIAVLMGVELMLNAVNI
                     NLIAFWRYLTPDRSVGLAWAVFVLTVAAAEAAAGLALIISVYRNRDSVDVEELDTLKN
                     "
     gene            5015..7279
                     /locus_tag="PLC06_04580"
     CDS             5015..7279
                     /locus_tag="PLC06_04580"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRO89752.1"
                     /translation="MNEQTLQLMTWLIPGMPLLAFFVIALVGYRNRTLSWVIAWIAII
                     SSLVMSWTVALNVLMRGLHALEEHPVQVSSMIDWIPLGDFFKGQWFRIGVVVDPLDAI
                     MLLMVSTATTMIFIYSVGYHNWGHGLGTHKGEPQHDMTEEPLLARFFGYMCLFGGSML
                     LLVVADNLLLLFVGWELMGFCSYSLVGFWYARQYPDPADMPIPRIPPRNAAVKAFMTT
                     RIADVVMLLGIVFMWATFGTLNFSEAFTVEGFEHAIEMVGFAGLGAMTLMLFTGTVGK
                     SAQWPLHVWLPDAMEGPTPVSAIIHAAAMVSAGIFMLLRIFPLIAVSLEGAPWVGLVI
                     AGIGAFTAIMAATIAVAQYDVKAVLAYSTISQLGFMVAAIGLGAYVAAAFHLITHAFF
                     KALLFLASGSVIHGMEHGAIHAHDHHHDPQDMRFMGGLRHKMPITFWTFLIGGMALSG
                     FPFITAGFWSKDEIFADAWYQWSHDQKILGLVVFITLAVAALLTAFYTMRQISLTFLG
                     KPRTELAEHASESSRFMTIPLMILAFFAVVVGWFGIPENFLGTDGIFTNFFHHYVGAQ
                     YFHLMEELHGLHLVGHTFETLPWSWMPLITSLVVALGGLALGWWVYARRPLRAGETDP
                     MVKTLGASTFRFLNNKWGWDGFYTRYFIKPTTWFSERVAYEFIDKGIIDGILHFVART
                     TYAVAAYFKLFEEKVISGGVDWIKDQFLSIAKEFRYLQTGKVQEYALISVFIATALAV
                     VILLINSGWLANIF"
     gene            7315..8859
                     /locus_tag="PLC06_04585"
     CDS             7315..8859
                     /locus_tag="PLC06_04585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRO89753.1"
                     /translation="MDAILNNLLILTIFSPVVAALVIFVMPSDAKNTIRRLALIFSLV
                     PLVLALVMWLNYNRVDAGLQYETLLPWFPAIGSSFHIGVDGISLAMVLLTVILTPLAI
                     LASFEIEDNVRMYMFLFLIMEAAMLGLFVSLDLIIFFIFWEFGLVPMYFLIKIWGGKD
                     REYASFKFIIYTMAGSLGLLLAIQVMGISMGTFDIPELMNTWVSLPAGSTLPTTGLSV
                     DLVKGISFWAFVIAFAIKVPIWPFHTWLPDAHTQAPTAGSMILAGVLLKLGAYGFIRL
                     VIPLFPEQATATAGILAILGMLSIVLGAFAAFGQWDFKRLVAYSSVNHMGFVVLGIAV
                     MAYAYGLPGQVEGLSYSAIIATNGAVLQMFNHGLSAAGMFFLVGVIYSRTHTRDLKQY
                     GGIWTSMPIYGMILIFTSMASLGLPGLNGFVGEFLVTRGAWPVFTVQLALSMIGLLMT
                     GAYILKVIYELLHGPLKPEWAGLSQMTLREHLVIWPLMALMLSLGVWPQWLLAVINDT
                     VTKLFS"
     gene            8882..10540
                     /locus_tag="PLC06_04590"
     CDS             8882..10540
                     /locus_tag="PLC06_04590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRO89754.1"
                     /translation="MEFPFLSIIALSPIFFAIVILMLPKDRGENARMLGLAAMVLGLI
                     LSIYVYVATYQSLPPAGTPWSETLRFVEEHPWVPSIGLNYIVGVDGLSATLVLLTAIV
                     GLGGVLISWSIDDRPREFYAFFMLLVAGVHGVFVAVDGFLLFFFYELAVLPMYVMIAV
                     WGWKERREYASMKLTLYLLIGSFISFISFLVLYFTPVEGGEALQTFDLRIWSQATFPF
                     DIQRIWFMPLFLGFGVLAGLWPFHNWSPDGHVAAPTAVSMIHAGVLMKLGAYASMRVG
                     IQLLPEGGVYWLPFILLLTLINVVYGALIAMRQKDLKYMIGYSSVSHMGLVSMGFAAM
                     VLVGFTGAGIQMVSHGVMTALFFAVVGMIYDRTHTRNMDDLSGMRKALPWTVVAFIIA
                     GLVGMGMPGLSGFLAEFPIFLGVWEGRGLDLTAVFGLNPSNYYGIVAIIAILGIVITA
                     AYILRAIHSVFFGEYEGEKWHDMRPLLAIDKFTLVMFSVILVVIGLFPMVIAPIVESG
                     MAPVADRLHVAQQAAQFHGGTITGAAQQMIQIVAANVMAWLGGA"
     gene            10541..12034
                     /locus_tag="PLC06_04595"
     CDS             10541..12034
                     /locus_tag="PLC06_04595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRO89755.1"
                     /translation="MSPNITLSWVLALAPEIGLLVVLFMVLVFDKLFKPADRRQVGLF
                     TAWGALAVLVMTVALYWVLDQPSAGWGVEDSLFWGGMMRNDLVTFVFRLMFLSALFLT
                     SLLSMDVPRLQKVEYFALLITATIGFSLMAASTDLIMVYMALETASISSYLLAGFYSG
                     QTRSSEAGMKYFIYGAFTTAVMVYGMSLLYGVTGQTSLYFIGDLFSGPEIAAVQPLVL
                     LAAVLITVGFAFKTSLVPYHFWTPDVYEGAPTPFVGMLSTASKAAGFAVLLRVFLSGT
                     LGPANTGSEWWAMLVAMCIITMTLGNLVAIFQTNIKRLLAYSSIAQAGYTLIGLVTLT
                     QDGSGASMFYLLMYVFTNIAAFGVIVIISNVTQSDELKDLSGLNRRSPFLALVMLVAV
                     LSLSGIPPTAGFFGKFFLFKAAIDAGFWWLALIAIVNAFIALYYYLGIIKYMYLYRSE
                     EDDVSIPVSRAAQVGLVVSTVFIIYLGVIAGPAFELTRQAAASFFPG"
     gene            12092..12385
                     /locus_tag="PLC06_04600"
     CDS             12092..12385
                     /locus_tag="PLC06_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89756.1"
                     /translation="MSQDPNLRQTAALANTVGQVGCVTGIVALVIIGAAFGAGWFLDD
                     FLGNERKIFTVIFMLGSFPVTLYAMVRISLWMVGKANKAIEQINQEDKDKNAI"
     gene            12382..13521
                     /locus_tag="PLC06_04605"
     CDS             12382..13521
                     /locus_tag="PLC06_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRO89757.1"
                     /translation="MKKRYILLILIAMMIAFGSVIAYTKPVLPFIQLPGEPYPGTEGM
                     MPAFLFNNAGLLNTFVAALVAWVVVLLLVFTLRAGSRTADEVPTGFYNVFEMILEGAY
                     NFAENIAGPKVREFFPYFMSFILIILISNWMGLIPGYDSIGLWENKPHFAGLKAESKA
                     LADAKAAGTELSQAELEEIMHEAEVTEDAANAWGLRDGLFLIRADNAIEGAPIKTDSH
                     GAPIGRNIEAADWTIVPFLRPASTDLNFTLAFALIAMVMVQYYGFKHLGFSYLNKFFP
                     FLEKNWVDKVKGDPIKAIDPAVGLLELVSEISKIISFSFRLLGNMFAGMVLLFVMGYI
                     LSVANLAFFGLELFVGVIQALVFALLTLIFMNSATESHGGGEEHH"
     gene            13611..13859
                     /gene="atpE"
                     /locus_tag="PLC06_04610"
     CDS             13611..13859
                     /gene="atpE"
                     /locus_tag="PLC06_04610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRO89758.1"
                     /translation="MDELSAAALAEGLKAIGAGLAMLGAIGAGAGIGILVGGAVQGIA
                     RNPDASGNIQTNMILGIAFTEAVAIYALVVALIILFVF"
     gene            13916..>14211
                     /locus_tag="PLC06_04615"
     CDS             13916..>14211
                     /locus_tag="PLC06_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89759.1"
                     /translation="MEALGINLGYLITQLLGITALLLILSAFVYKPMLRVLDERKARI
                     AKGLEDARQAEIARQNADADAKRILDEARAEAANLRREAVVAAEGAAKDVEHKAR"
BASE COUNT         2461 a         4145 c         4253 g         3352 t
ORIGIN      
        1 cattccgcga actcgtattt cgtatcccgt atttcgtatc tcgcttatgt ctgaagttga
       61 aaccattgat gtgctgatcg tcggttccgg tccatccggc acgtcgacgg cgctacacct
      121 gatcaagcac gatccatcct gggcaaaacg catcgtcatc gtcgacaagg ccgtccaccc
      181 gcgcgagaag ttgtgcggcg gcggcatcac ccatctgggg cggaatgtgc tgtccgacct
      241 ggggttgacg ctggagccgc gccatttcga cgtgaccgaa gcccggctcg tctatcatga
      301 caagagctat tccttcttca gtgacccggt attcaccatc gtccggcgcg acgagttcga
      361 ccattggctg gttaagacgg ccgagaagct tggcgttctc gtgcggcagg gggaagcggt
      421 caaggagatc acgcctcatg ccgattacgt tgaggtcgtg accgaactgg cgaccttccg
      481 cgccaaagtc gttgtcgctg ctgacggctc gcgcagctac gtgcgccgca agctgaagtg
      541 ggccggcgac gaacccggca ccaatgagcc tcatgtggcc cggctgatcg aggtgctgac
      601 gcccgaagac ccggccacta cgccggagtt ccgcgacggc gtggccgtgt tcgacttcac
      661 gccgatggac gacggcctgc aggggtacta ctgggatttc cccagtttca tcaagggcaa
      721 ggcctatatg aaccggggcg tcttcgacag ccgcgcccgg ccggagaagc ccaaggctga
      781 tctgaaggag attctggccg gggagttggc tgagcgcggc cggcaactcg acgactacga
      841 attgaagggc caccccattc actggtggaa cgcgcggggc aagttcgccc ggccgcgggt
      901 gctgctggtg ggcgatgccg ccggagccga cccgctgatg ggcgaaggca tctcttttgc
      961 gctgggctat ggcgacccgg cggccgcgac catcatcgac gccttcgccc acgacaactt
     1021 cagcttcgac tcctaccgcg agcggttggc ggcccatccg ctgttccggc aacttgaccg
     1081 gcgggtgcgg ctggcgcgct atgcctattg gctcaatcgg ccgtggctgg ccggcctggg
     1141 ctggcgcgtc atgcggctgg tgatgcgctt cacgtcgtgg agcgaccgga gccatgtgcc
     1201 cattcgcgag ccggagttct ccctaatcga ggcgagcaag acagtctgac gccattcaac
     1261 cgaggttctg gaacgtatac ccaatccccc gctcggccag aatatagacg gggttgttgg
     1321 ggtcttgctc gatcttgccg cgcaggcggt gaacgtggac gtggagccgg tcctcgcgcg
     1381 cctcttgcag tggccagatg cgattgagca ggaagtcggt ggtgacgatg cgcccgccgt
     1441 tgcgcacgag gatgtagagc agcttcgtct ccgtcggcgt cagcgacacg gcttgcccgt
     1501 cgaccgtcgc ctcgcgcagg gggaagttga tcagcagccg gtcgtcgatg cgcgtcgtcg
     1561 attccagggt gtaggtgtag tcttccatgc ggccgagcac gcgccgcacg cgagcgatca
     1621 actccggcgg gttgaacggt ttgaccatgt aatcctcggc gtgctcctca agcccttcga
     1681 cgatcgtttc ctcggtgttg gcggccgtca gcatgatgac cggcacatca ctgaactcgt
     1741 ggatcgatcg gcagaactcg aagccgctca tatcgggcat gtgcagatcg acgatggcca
     1801 ggtggggcag gccatggcgg ttgatgagct tcagcgcctc ggggccggag acggccgtgg
     1861 agacctcgta gccgccctgt tccagggtgt gctgcacgat gcgcagagtg taggtattgt
     1921 cgtcaaccac caggatgcgc tgggtttcgg gaggggattc aagcatagca gttcattgct
     1981 ccggcggccg cgtggacgcg acctgaccaa taatggtaaa ccaaactgcc aaggatcgcc
     2041 aatccgcaga attccatcgg gtccgcaagc aggtgaattg aaacctcttt cttctaatag
     2101 gcgctttgct cgtcagcctg tcgagtcttg gggctacgaa aaaacctcaa aaagcctatg
     2161 tcatttgttg cgctattgac ttcatgtgct ataaatgaga ggaatttcac aattacgctc
     2221 taccttccag gagaggatga ggaaccactg tgcaggctga ttggcaatac atcgcagttt
     2281 ttgtggcgct ttcgccaata ttccctttgg cccctgtggt gctgaaccgt ttgctcggtc
     2341 cacaccggcc aaatccgatt aaacagcaga cctacgagtg cggcgttgag acggtaggcg
     2401 acacctggat ccagttcaag gtccagtatt atatttatgc cctggtcttc gttgtgtttg
     2461 atatcgaagc cgtcttcctg ttccccatcg ccgccgcgta tggacaattg gacctgttcg
     2521 ccatcatagc taccgtgctg ttcattttcc tgttggctga cggtctgatt tacgcatgga
     2581 gccgcggagt tctggagtgg gtctaacgcc catcacccaa cattgacaga ggagataccg
     2641 gcgcgcgaac aggcacgccg tttttccctg aaatgaaaag gcgcatccgc tatgaatatg
     2701 attattgacc tgttaaagag tctaatttca ttgagcctgg gcgattttat tcgccgcaac
     2761 ttcggcacgg ggccgggtac ggaactgctg atcgacattg gcggcgtggt catcttgtcc
     2821 acgttctgtc tgttgatcgt catcttcctc atctggctga cacgcaagct agtggcccgc
     2881 atgcaggacc gcatcggtcc gaaccgtgtc ggcggccgct atggtctgct gcagacggtg
     2941 gccgacgtgg tcaagctgct gtcgaaggaa ctcatccacc cgtccggtgc cgactggctt
     3001 tcctataacg ccgcgcctat tctgacggtt atggcggcgc tgctggtgtg ggcggtcatg
     3061 cccttcgcgc ccggcgtcat cggcgtcgat ctgaacatcg gcgtgttcta tatcatggcc
     3121 atcagttcga tatcggtcgt ggtgctgctg ctggcggggt ggggttcgaa taacaaatac
     3181 gcgcttctgg gcgcgttccg cgccgtggcg cagatggtca gctatgaagt gccgatgatg
     3241 ctggccctgt tagtgccggt catcctcgcc cggaccatgt cgaccaaggg gatcatcgac
     3301 gcgcagaatt tgcctttcat tctgttcgcg ccggtctcgg ccctcatttt ctacatctcc
     3361 accctcgccg aaacagggcg cacgccgttt gacctgctgg aggcggagtc ggagatcgtg
     3421 gccggcttcc atattgagta cggcggcatg aagttcggca tgttcttcct ggccgagttc
     3481 gtcaacacgc ttttctggtc gggcatgttc gtcacccttt atctgggtgg ctatcgcatc
     3541 ttcgggctgg agaactgggt cggtggcgac ggctacccct acggccggtt gatcggcctc
     3601 atcgccttct tcgccaagac ctttttcatg tatttcgtct atgactggat ccggggcacg
     3661 ttgccgcgcg tccgcgtcga ccagattctt aacttcaact ggaagttcat ggtgccgctg
     3721 tcgctggtgc tgatcttctt tgtcgctctg gtggataaat ttatccccga caccgccggc
     3781 acagtgacgc ggatggccat ccatttgacg ctaaatctcg tcattgcagc cgcgacgctg
     3841 gagttcttgc ggcggcgtgg ccgtcaattc cgtcaggaga tgtccggcgg cgatggcccg
     3901 ggcggccgtg agactttgta cccggatgat catggccacg gggcggccca cgaagcaccg
     3961 gcccatagcg atcatggcga tatgccgctg ggcggccgcg aacccatcgc ggtgccgctg
     4021 cactagattc caatttacac tgaggcgcaa gcatgagcca gcagataatc tttttcatcc
     4081 tcgccgccat cacgctgact tcggccatcg ccgtggtcac aatgcggaac cttttccacg
     4141 cggcgttggc cctgatggtg agtttcctgg gcgtggcggg gatatacgtt ctcctggaag
     4201 caggattcat gggcatggcc cagcttttgg tctatatcgg cgcgatctcc attctgatta
     4261 tcttcgccgt catgatgacc cgacgcatga tgcagacgcg ggagacgccc ttcaacgcgc
     4321 aaacgctcgc cgcgctagtc ggaacgctga tcaccatggc catcctcttc gtggtcattg
     4381 gccggctcta tccgctggcg cccaccacgg agatcccgct cggcgcggcg ccgcaagtcg
     4441 atccggccat cattggcagc agcatcgatc aattgggacg ggcgtttgtc ggcgccaacg
     4501 cctatgtatt gccgttcgaa gtcgcctcgg tgctgttgct ggcggcgctg gttggttcca
     4561 tcatcatcgc ctggccgcgc agcggcgagg aggataacct gtgatccctc tttcctggta
     4621 tttgctgctg agcgcggcgc tcttttcggt cggcctgttc ggcgtgctgg cgcggcgtaa
     4681 tgccatcgcc gttttaatgg gcgttgaatt gatgctcaac gccgtgaata ttaatttgat
     4741 cgctttctgg cgctacctga cccctgatcg ctcggtaggg ctggcttggg ccgtgtttgt
     4801 gctgaccgtg gccgcggcag aagcagccgc cggtctggcg ctgattattt ccgtttatcg
     4861 caaccgtgat tcggtcgatg ttgaggaatt ggacacatta aagaattagg aatcaggaat
     4921 taccgggttg agaaatgggg ataggcttcg atggctttct tttctttatt cctaattcgt
     4981 aattcataat tcataattcc cttcggtact gcctatgaac gagcagacac ttcaattaat
     5041 gacctggttg ataccgggga tgcctctcct ggcatttttc gtcatcgctc tggttggcta
     5101 ccgcaaccgg acgctgagtt gggtcatcgc ctggatcgcg attataagct cgctggtcat
     5161 gagctggacc gtcgccctca atgtgttgat gcgcggtttg cacgcgctgg aagagcatcc
     5221 ggtacaggtc tccagcatga ttgactggat tcccctgggc gacttcttca agggtcaatg
     5281 gttcaggatc ggggtggttg ttgatccact cgacgcgatc atgctgctga tggtctccac
     5341 ggccacgacg atgatcttca tctatagtgt tggctatcac aactggggtc acgggctggg
     5401 cacgcacaag ggcgagccgc agcacgatat gaccgaagag ccgctgctgg cgcgcttctt
     5461 cggctatatg tgcctttttg gcggctccat gctgctgctg gtggtggccg ataatctgtt
     5521 gctgctcttc gtcggctggg agttgatggg cttttgctcc tactcgctcg ttggcttctg
     5581 gtatgcgcgg caatatcccg acccggcgga tatgccgatc ccgcgcatcc cgccgcgcaa
     5641 tgcggccgtc aaggcgttca tgaccacccg tattgccgac gtggtcatgc tcctgggcat
     5701 cgtcttcatg tgggcgacct tcggcacgct gaatttcagc gaagccttca ccgtggaagg
     5761 gtttgagcat gccatcgaga tggtaggctt cgccgggctg ggggccatga ccctgatgct
     5821 gttcaccgga acggtcggca agtcggccca gtggccgctg cacgtctggt tgcccgacgc
     5881 gatggaaggc ccgacgccgg ttagcgccat catccacgcc gcggccatgg tctcggccgg
     5941 catcttcatg ctgctgcgta tcttcccgct gatcgccgtc agcctggagg gcgcgccgtg
     6001 ggtggggctg gtcatcgccg ggatcggcgc gttcacggcc atcatggcgg ccaccatcgc
     6061 cgtggcccag tatgacgtga aggccgtcct cgcttattcg accatctccc agttgggctt
     6121 catggttgcc gccattggtc tgggagccta tgtcgccgct gccttccatc tgatcaccca
     6181 cgccttcttc aaggcgctgc tgttcctggc atccggctcg gttatccatg gcatggagca
     6241 tggcgcgatt cacgcccacg atcaccacca tgacccgcag gacatgcgct tcatgggcgg
     6301 cctgcgccac aagatgccga tcaccttctg gacgttcctg atcggcggca tggccctgtc
     6361 gggcttcccg tttattaccg ccggattctg gtcgaaggac gagatcttcg ccgacgcatg
     6421 gtatcaatgg tcgcacgacc agaaaatatt gggcttggtc gtcttcatca ccctggctgt
     6481 ggccgcgctg ctgaccgcct tctacaccat gcgccagatc agtctgacat tcctgggcaa
     6541 gccgcgcacc gaactggccg aacatgccag cgaaagctcg cggttcatga ccatcccgtt
     6601 gatgattctg gccttcttcg ccgtcgttgt gggctggttt ggcatccccg aaaactttct
     6661 gggcacggac ggcatcttca ccaacttctt ccatcattat gtgggcgcgc aatacttcca
     6721 tttaatggaa gaactacatg gcttgcatct ggtggggcat acgtttgaga cgctgccgtg
     6781 gtcgtggatg ccgcttatta catccctcgt cgtcgccctg ggtggcttgg ctttgggctg
     6841 gtgggtctat gcccggcggc cgctacgggc cggggagacc gatccgatgg tcaaaacgct
     6901 cggcgcttcg acgttccgct tcctgaacaa caagtggggt tgggacggtt tctataccag
     6961 gtactttatc aaaccgacga catggttctc ggagcgggtg gcctacgagt tcatcgacaa
     7021 ggggatcatc gacggcatac ttcatttcgt ggctcgcacg acctacgctg tggccgccta
     7081 tttcaagctc ttcgaggaaa aggtcatcag cggcggtgtc gactggatta aggatcagtt
     7141 cctgtccatc gcgaaagagt tccgctattt acagacgggc aaggtgcagg agtatgccct
     7201 gatttcggtc tttatcgcca cggcgctggc ggtagtcatc ttattgatca acagcggctg
     7261 gctggccaat atattctgat ataggcagtc tgaataccgg ttggcgacta gactatggac
     7321 gctattctca acaatcttct gatactgacc atcttttccc cggtggtcgc ggcgctggtc
     7381 atcttcgtga tgccctccga cgcgaagaac accatccggc ggctggcgct gattttcagc
     7441 ctggtgccgc tcgtgctggc tctggtcatg tggctcaact acaaccgcgt ggacgccggg
     7501 cttcagtatg aaaccctgtt gccctggttc ccggccattg gctccagctt ccacatcggc
     7561 gtcgacggca tcagcctggc gatggtgctg ctgacggtca tcctgacgcc gctggccatt
     7621 ctggcttcct tcgagattga ggacaatgtg cgcatgtaca tgttcctctt cctgatcatg
     7681 gaagcggcga tgctggggct gttcgtctcg ctcgacttga tcatcttctt catcttctgg
     7741 gagttcggcc tcgtgcccat gtacttcctc atcaagatat ggggtggcaa ggatcgggaa
     7801 tacgcatcgt tcaagttcat catctacacc atggccggca gtctgggcct cttgctggct
     7861 attcaggtca tgggcatctc gatggggacg ttcgacatcc ccgaactgat gaatacctgg
     7921 gtcagcttgc ccgcgggcag cacgctgccg acgacggggc tatcggtaga cctggtgaag
     7981 ggcatctcct tctgggcctt cgtcatcgcc ttcgccatca aggttcccat ctggccgttc
     8041 cacacctggc tgcccgacgc ccacacgcag gctccgacgg ccggctcgat gatcctggcc
     8101 ggcgtgttgc tgaagctggg cgcgtatggc ttcatccgcc tggtgatccc gctcttcccg
     8161 gagcaggcta ccgctacggc gggcattctg gccatcctgg gcatgctcag catcgttctg
     8221 ggcgcatttg ccgccttcgg ccaatgggat ttcaagcggc tggtggctta ctcctcggtc
     8281 aaccatatgg gctttgtcgt cctgggtatc gccgtcatgg cctatgccta cggattgccc
     8341 ggccaggtgg aagggctgag ctacagcgcc atcatcgcca ccaacggcgc ggtgctgcaa
     8401 atgtttaatc acggactctc ggccgcgggg atgttcttcc tcgtcggcgt catctattcg
     8461 cgcacgcaca cgcgcgacct gaagcagtac ggcggtatct ggacatccat gcccatctac
     8521 ggcatgattc tcatcttcac cagcatggct tcgctcggcc tgcccggcct caacggcttt
     8581 gtgggcgagt tcctggtgac tcgcggcgcg tggccggtgt tcaccgtcca gctggcactg
     8641 tcgatgatcg gtctgctcat gaccggcgcc tatatcctga aggtaattta tgaattgctg
     8701 catgggccgc tcaaaccgga atgggccggg ctgtcccaga tgacgttgcg tgaacatctt
     8761 gtcatatggc cgctgatggc cctgatgctc agtctgggcg tctggccgca gtggttgctg
     8821 gctgtgatca acgacacggt gaccaaactc ttcagctagt cgctggagcc ggacgaaaac
     8881 tatggaattc ccgtttctgt caatcatcgc cctgtcgccg attttcttcg cgattgtgat
     8941 cctcatgttg cccaaggatc gcggagagaa cgcgcgcatg ttggggctgg cggctatggt
     9001 gctgggcctg atcctgtcga tctacgtcta cgtggcgacg tatcagagtc tgcccccggc
     9061 cggcacgccc tggagcgaaa cgctccgctt cgttgaggaa cacccctggg tgccgagcat
     9121 cgggctgaac tacatcgtcg gcgtcgacgg cctcagcgcc acgctggtgc tgctgacggc
     9181 catcgtcggt ctgggcggcg tgctcatctc gtggagcatc gacgatcggc cgcgcgagtt
     9241 ctacgccttc ttcatgctcc tggtggccgg tgtccatggc gtctttgtgg ccgtcgacgg
     9301 ctttctgctc ttcttcttct atgaactggc cgtgctgccc atgtatgtca tgatcgccgt
     9361 ctggggctgg aaggagcggc gcgaatacgc ctccatgaag ctgacgctct atctgctcat
     9421 cggcagcttc atctcgttca tctccttcct ggtgctctat ttcacgccgg tggaaggtgg
     9481 cgaggcgctg cagacctttg acctgcggat ttggtcgcag gcgaccttcc cgttcgacat
     9541 ccagcgcatc tggttcatgc ccctcttcct gggcttcggg gtgctggccg gtttgtggcc
     9601 gttccacaac tggtcgcccg acggccacgt ggctgcgcct acggccgtgt cgatgatcca
     9661 cgcgggtgtg ctgatgaagc tgggcgccta cgcttccatg cgcgtcggca tccagttgct
     9721 gccggagggg ggcgtctatt ggctgccctt catcctgctg ttgaccctga ttaacgtggt
     9781 ctacggcgcg cttatcgcca tgcgccagaa agacctgaag tacatgatcg gctattccag
     9841 cgtcagtcac atgggactgg tttcaatggg ctttgcggcc atggtgctcg tcggtttcac
     9901 cggcgcgggc atccagatgg tcagccacgg cgtgatgacg gcgctcttct tcgccgtcgt
     9961 cggcatgatc tacgaccgca cccacacccg caacatggac gacctgagcg gcatgcgcaa
    10021 ggcgctacca tggacggtgg tcgcgttcat catcgccggc ctggtcggca tgggtatgcc
    10081 cggtctgtcg ggcttcctgg ctgagttccc catcttcctg ggcgtctggg aaggacgggg
    10141 gctcgacctg accgcagtgt tcggtctgaa cccgtcgaac tactacggga tagtggctat
    10201 catcgccatc ctgggcatcg tcatcaccgc ggcctacatt ctgcgggcca tccattcggt
    10261 cttcttcggc gaatacgaag gggagaaatg gcatgacatg cggccgctgc tggccatcga
    10321 caagttcacg ctcgtcatgt tctccgtcat cctggtcgtg attggccttt tcccgatggt
    10381 catcgcgcct atcgtagagt cggggatggc accggtcgcc gatcggttgc atgtagcgca
    10441 gcaagcggcc caattccatg gcggaaccat caccggggcc gctcaacaaa tgatacagat
    10501 cgtcgcggcc aacgtcatgg cctggttggg aggtgcataa gtgagtccca atattaccct
    10561 ttcgtgggta ttggcccttg ctccggagat cggcctgctg gtcgtcctgt tcatggtgct
    10621 ggtatttgac aaactgttca aaccggcgga caggcggcag gttggcttgt tcacggcctg
    10681 gggcgcgttg gctgtcctgg tgatgaccgt cgcgctgtac tgggtgcttg accagccaag
    10741 cgccgggtgg ggcgtggaag acagcctctt ctggggcggc atgatgcgta acgacctggt
    10801 gacgttcgtc tttcgcctga tgttcctgtc ggcgttgttc ctgacgtcgc tcctgtcgat
    10861 ggatgtgcca cggctgcaga aggtcgaata tttcgcgctg ctcatcacgg cgaccatcgg
    10921 attcagcctc atggcggcct cgacggacct gattatggtc tacatggcgc tggagacagc
    10981 cagtatctcg tcttatctgc tggcggggtt ctatagcggc cagacgcgct cgtccgaagc
    11041 gggcatgaaa tatttcatct atggcgcgtt caccacggcg gtcatggtct atggcatgag
    11101 cttgctctat ggcgtgacgg ggcagacaag cctgtacttt atcggcgatt tgttctccgg
    11161 cccggaaatc gccgccgttc agccgctggt gctgctggcg gccgtgctga ttaccgttgg
    11221 cttcgccttc aagacttcgc tcgtccccta tcacttctgg acgcctgacg tctatgaggg
    11281 cgcgccgaca cctttcgtcg gcatgctgtc cacggcctcg aaggcggccg gctttgccgt
    11341 gctgttgcgg gtgttcctgt ccggcaccct cggcccggcg aacaccggct cggagtggtg
    11401 ggccatgctc gtggcgatgt gtatcatcac catgaccctg ggcaatcttg tcgccatctt
    11461 ccagaccaat atcaagcgtt tgctggccta ctcgagcatc gctcaggcgg gctacacgct
    11521 cattggcctg gttacgctga cccaggacgg gtcgggcgct tcgatgttct acctgttgat
    11581 gtatgtcttc accaacatcg cggctttcgg cgtgatcgtc atcatcagca atgtgaccca
    11641 atccgacgag ttgaaagacc tgtccggcct caaccgccgg tcgccgttcc tggcgctggt
    11701 gatgttggtg gcggtcctgt cgctcagcgg tatcccgccg acggccggat tcttcggcaa
    11761 gttctttctc ttcaaggcgg ccattgacgc ggggttttgg tggctggccc tcatcgccat
    11821 cgtcaatgct ttcatcgcgc tctactacta tctgggaatc atcaagtaca tgtatctgta
    11881 ccggtcggaa gaagatgatg tcagcattcc cgtgtcgcgc gcggcacagg ttggcctggt
    11941 tgtctcgacc gtgtttatca tttatctcgg cgtgatcgcc ggtcccgcat tcgaactgac
    12001 gcggcaggcg gccgcctcgt ttttccccgg ttaagattga ccggaagttc gatcgcggat
    12061 acaatgcgca agctaaccta aacaatccga cgtgtctcaa gatccgaatc tcagacaaac
    12121 ggccgccctg gctaacacag tgggtcaggt ggggtgcgta accggaatag tcgccctcgt
    12181 aatcatcggc gccgcctttg gcgcgggatg gttcctggac gactttcttg gtaatgagcg
    12241 caagatcttc accgtcatat ttatgctcgg gagctttccc gtcactctct atgccatggt
    12301 gcgcatcagc ctgtggatgg taggcaaggc gaataaagcg attgagcaga taaaccagga
    12361 agacaaggac aagaacgcaa tatgaagaag cgctatatcc ttctgatcct tatagcaatg
    12421 atgatcgcct tcggctcggt gatcgcttat accaagcccg ttttgccctt catccagttg
    12481 cccggcgagc cgtatccggg taccgaaggg atgatgccgg catttttgtt caacaatgcc
    12541 gggctgctca acacgtttgt ggcggctttg gtggcgtggg tcgtcgtgtt gttgctggta
    12601 ttcaccctgc gcgccggttc gcgcacggcc gacgaagtgc caaccggttt ctataacgtg
    12661 tttgagatga ttctcgaggg agcctacaac ttcgccgaga atatcgccgg gccgaaagtg
    12721 cgggagttct tcccctattt catgagcttc atcctgatca tcctgatctc caactggatg
    12781 ggccttatcc ccggttacga cagcatcggg ctgtgggaga acaagcccca cttcgccggc
    12841 ctgaaggcgg agagcaaggc gttggccgat gccaaggctg ccggcaccga gctgtcgcag
    12901 gcggagctgg aagaaatcat gcacgaggct gaggtgaccg aagacgccgc caacgcctgg
    12961 ggtctgcgtg acgggctgtt cctgattcgc gccgacaacg ccattgaagg cgctccgatc
    13021 aagacggatt cccacggcgc acctatcggc cgcaacatcg aggcggccga ctggaccatc
    13081 gtcccgttcc tgcgcccggc ctcgaccgac ctgaacttca ccctggcctt cgcccttatc
    13141 gccatggtca tggtgcagta ttatggcttc aagcacctgg gcttcagcta tctgaacaag
    13201 ttcttcccgt tcctggagaa gaactgggtc gacaaggtga agggtgaccc gatcaaggcc
    13261 atcgacccgg ccgtcggcct tctggaactg gtcagcgaga tttcaaagat tatctcgttc
    13321 tccttccgtc ttctcggtaa tatgttcgcc ggtatggttc tgctattcgt catgggttat
    13381 atcctgtcgg tggcgaacct ggcgttcttc gggctggaat tgttcgtggg tgttatccag
    13441 gcgctcgtct ttgccctgtt gacgctcatc ttcatgaaca gcgcgaccga gagtcacggt
    13501 ggcggcgaag agcatcatta agaagcacgg gcaatgccgg aacacgagtt cccatgccca
    13561 acacacaaaa aattgtagat aatattcaaa gtctatcgga ggactaacac atggatgaat
    13621 tatcagctgc agcactcgcg gaaggcctga aggctatcgg cgcaggtttg gctatgttgg
    13681 gcgccatcgg cgccggcgcc ggcatcggta ttctggttgg tggtgctgtg caaggtatcg
    13741 cccgcaaccc ggacgccagc ggcaatatcc agacgaacat gattctgggt atcgctttta
    13801 cggaagctgt cgctatttac gccctggtcg tggctctgat cattttgttc gtgttctaag
    13861 aggatacccg gagattggtt ggattatgcc gaccaatttt ggatgaggat tacttatgga
    13921 agcattaggt attaacttag gttacctgat cacgcagttg ctgggtatca ccgcgttgct
    13981 gttgatcctg tccgcgttcg tctacaagcc gatgttgcgc gtgctggacg agcggaaggc
    14041 acgcatcgcc aagggcctgg aagacgcgcg acaggcggaa atcgctcgcc agaacgccga
    14101 cgccgacgcg aagcggattc tggacgaagc gcgcgccgaa gctgccaacc tccgccgcga
    14161 ggcggttgtg gccgccgagg gcgccgcgaa ggatgtggag cacaaggcgc g
//
LOCUS       DAOMQG010000049        13957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1881_length_13957_cov_10.983887, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000049 DAOMQG010000000
VERSION     DAOMQG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1881_length_13957_cov_10.983887"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLC06_04620"
     CDS             complement(<1..251)
                     /locus_tag="PLC06_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89760.1"
                     /translation="MNNSQIKRMWVIALVIAGAGLVIVGRLFAFQLTQGEEFKKVLIK
                     ELPVTDQPERGVIYDRNGAILAVDRWDYRVAVSPSVLTDP"
     gene            complement(241..732)
                     /locus_tag="PLC06_04625"
     CDS             complement(241..732)
                     /locus_tag="PLC06_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRO89761.1"
                     /translation="MMATPEEKRRPRQARELARRLGLLTQAQAATGWAVILILGALLG
                     VIYLNQASKIAAIGRHVQRVQADLDEVKRTNAELERQIAEAQALDRLEAEARRLGFVP
                     AAPGDIEYIVIPNYPTEPTVERAEPTGPLVQPEPPASMWEAIVLAVRDLGIDLTRGEA
                     NEQ"
     gene            complement(729..1694)
                     /gene="rsmH"
                     /locus_tag="PLC06_04630"
     CDS             complement(729..1694)
                     /gene="rsmH"
                     /locus_tag="PLC06_04630"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRO89762.1"
                     /translation="MVDDAERTGEYGHLPVLLREVLAYLDPQPGDLFIDGTLGAGGHA
                     SAILDLIAPNGRLLAFDRDPEAMQFATRRLDSCGNRLTPVVASYADMGTIAPEHGFAA
                     VDGILLDLGLSSRQLEDANRGFSFLKEGPLDMRFDPRQGGTAADLINNLTAEELADIF
                     RRYGEETHSRRIARLIIANRPLQTTTELADLIENEIGRRGRPGRHPATQVFQALRIAV
                     NGELNEVERGLTAATRLLKPGGRLAVISFHSLEDRLVKNFFRDAARACVCPPEQPVCT
                     CGARPELKLVTRKAVQATESEIAANPRSRSARLRVAAKSDNEDSE"
     gene            complement(1751..2170)
                     /gene="mraZ"
                     /locus_tag="PLC06_04635"
     CDS             complement(1751..2170)
                     /gene="mraZ"
                     /locus_tag="PLC06_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRO89763.1"
                     /translation="MFLGEYRHTIDDKGRLTIPAKYRGLLAAGMVVTRGFDRNLMAFS
                     MEGWEELATRVKSLPWSDPSAREFRRRVFSGAIDLVPDRQGRVLLPPYLREFANIDSD
                     VVITGMLDHLEIWNSDAWEPVREAAESDEQHWENLGI"
     gene            complement(2561..3451)
                     /locus_tag="PLC06_04640"
     CDS             complement(2561..3451)
                     /locus_tag="PLC06_04640"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89764.1"
                     /translation="MVLVILKLLVVLFFLAMFVRKPNVAWGIGLLTVTTAVLLDTLLG
                     TFNREELLAELGFFFYVISGVLLAGSAAWFWGVVRPLLARDMTPATATPMIASAARTT
                     APAPVIAAATAAAPVPSKSKLPPPLPPDHVDQYAEAGFDRQMLYEEMRQRFSRADLSD
                     LMFDLNINEADIATGGRNLDELIVAVMDAAELDGNAPTVALAVERVLTPPPPEHLPRL
                     ERLSADSPRTVLRHYLLANFSPEELKQLAADLGIDWEELEGSDTESRVRAMLLNLYRH
                     DRVETLLEAMRGDEPEETAN"
     gene            3606..4070
                     /locus_tag="PLC06_04645"
     CDS             3606..4070
                     /locus_tag="PLC06_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HRO89765.1"
                     /translation="MANTNKPSGEEIKDIGFWGEVWQQIRLVFYLIKDREVPIYLKAL
                     PFLGVAYMLFPIDIITDFVPVLGQLDDLTLLLIGGKVFIEMSPPQVVARYLDQMKAAR
                     TATIIEGEVSDAADDPLAHMTKGIKLIEGELSEAASPIEEGEPAAKKSWFRR"
     gene            complement(4067..4780)
                     /locus_tag="PLC06_04650"
     CDS             complement(4067..4780)
                     /locus_tag="PLC06_04650"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO89766.1"
                     /translation="MNDTSHRLLIQSGPNAGQEYQLVAPSITIGRYPLADIVINDPDI
                     AYRHALLTRDDTSYRIADLGSDSGTYVNGERVGAEPIALAHGDIILLGSRLSATYLEH
                     AIETAIDEYPAAVTSLVSETEVSNGGEGVVALDELGHIGATNGDEYEPPVVEERVVSS
                     ESSPVTAEHPVLTEPAHIEHHPGPMPVMPAPQKNKPGRIILIATGCMIALLACCCSTT
                     LFMYFIGGDWLLRQLGYLP"
     gene            complement(4800..6452)
                     /gene="murJ"
                     /locus_tag="PLC06_04655"
     CDS             complement(4800..6452)
                     /gene="murJ"
                     /locus_tag="PLC06_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRO89767.1"
                     /translation="MEQIPDPTTPEGLPVPAEIDGDAFLSEEYRQQATGDRPQTSHES
                     GIVRAAAVLAAGNVASRVLGLAREMVKANLFGTSPLLAAFQAAAYVPTSLFDLIIGGM
                     VNSSLVPVFSDYADKEKRDELWQVLSMVLSAATLVLLGVIAVVELLAPQVAWLVGAAN
                     FAAADLSDFTVTLIRMTTPAVFFLGIASVLTGALYALKRFTLPAFVGAAFNGTIVIVA
                     LLNPTDIRSLVWGLLLGSLLQVLLQLPALRDARLRWRLDFQHPAIRRIIKLYIPILAG
                     LVVNQLAIMLSYNLAIRTGDQSLNYMNYATTLYQFPLGLVVTALSIATLPTLSRQAVA
                     DLAQFKQTLADGLRLVLALILPATTGLFALAPVIIGLLFEHGRFTALDTATTALVLRI
                     YLFGMPFAAADQMLVFASYARKDTWRPALVGFISILIYSLTALLLLRPLGLLSLMVAD
                     AVKHVVHTFIMLWVLRRQLGGFASDPVLRSLAKSLIAAILTGFAAFGAAQLAVGWTVS
                     PTIARLGPVAIGGLAGIAAYTICVFALNITETRDLPRLFARR"
     gene            6648..8330
                     /locus_tag="PLC06_04660"
     CDS             6648..8330
                     /locus_tag="PLC06_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRO89768.1"
                     /translation="MYDKAKLAELAEARDRWEETTLQQTLARAPERKEQFVTTSGEPI
                     RRLYTPLDVADLDYLGDLGNPGDYPYTRGVHSTLYRGKPWTMRMFAGFGSAEETNARY
                     KYLLSQGNMGLSVAFDLPTLMGYDTDAPQSLGEFGKCGVAISSLADMEILFDGIPLDQ
                     VTTSMTINSPAAIIWAMYIAAAEKQGVPQAKLGGTLQNDILKEYIAQKEYIFPPEPSM
                     RLVIDTIEYGARHMPLWNTISISGYHIREAGSTAAQELAFTLGDGLEYVRWAIARGMD
                     VDEFAPRLSFFFNSHNDFFEEIAKFRAARRIWAREMRETFGAKNPRSWLCRFHTQTAG
                     VSLTAQQPETNVVRVAIQALAAVLGGTQSLHTNSMDEALALPSEEAVTVALRTQQIIA
                     EESGVVNTVDPLAGSFFVEHETNKIEAQVYDYWRQVDELGGVLPAIEQGFYQSEISVA
                     AYRYQREIDRNERYIVGVNAYADPDEKLRIPILAMDPNGYDRQIARLKRLREERDNER
                     VSETLAALRSAAAGTENTMPYILDAVRAYATLGEITDVFREVFGRYEEPTWI"
     gene            8504..8641
                     /locus_tag="PLC06_04665"
     CDS             8504..8641
                     /locus_tag="PLC06_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89769.1"
                     /translation="MTIKQVGGRADADLFYPPGNYTAYASGVADPDTALSEIDASWLT
                     E"
     gene            8744..9907
                     /locus_tag="PLC06_04670"
     CDS             8744..9907
                     /locus_tag="PLC06_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRO89770.1"
                     /translation="MQSESAQEQSGLVARLLNLALPVAALIIALVAMYLGFDFLRAGS
                     APKWLIVLVAIVWGVGGVAALYWILNWIVERMNLKWRGRLQPFVFVGPAIVMLMWFLA
                     VPVLRTFWLSLFNRDGPPIGWLNWSDIGGSFASLSGRFVGLDNYVAVFTDRLMREAFR
                     NNIMWIVFGATLTVIVGLLIAVLADRSSFERVSKSAIFLPMAISFVGAGVIWNFIYEV
                     RPVDVPQIGLLNAVVVGLGGKPQPWPAWTAISPWNNLFLIVIVIWLQVGYAMTLFSAA
                     LKGIPADLLEAGRVDGANEIQIFFRIMVPYIMGTIITVSTTVVIFTLKVFDVVWVMTG
                     GQFGTEVIATQFYRQSFTARNSGYGSAIAIVLLIAIVPVMIYNLKQFREQEAF"
     gene            9909..11015
                     /locus_tag="PLC06_04675"
     CDS             9909..11015
                     /locus_tag="PLC06_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRO89771.1"
                     /translation="MGSIQNQRRWGSLLVNIGLLLLVLFWTIPTFGLLVSSFRTRESI
                     QASGWWNVFPHRAYETVATLSPAEMGLDPTGPMVIEGVETTFEEMRKTGITTPDGTFY
                     RWVGNRRIGRVEIQEQSWTVDWDFTLDNYRQVLSGKNFEYKQPDGTIKVIPGDNFISA
                     FLNSLAVAVPATVIPILVAAFAAYGFAWMNFPGRRMLFIMVVALLVVPLQLALVPILR
                     DFRSLQINGTFMAMWLAHTGFGLPLATYLLFNYISTLPRETLESAFIDGASHFVVFRK
                     LILPLSVPALASFAIFQFLWVWNDYLVALIFLGGSPETKVITQRLADIVGSRGNDWHL
                     LTAGAFLTMLLPMIVFFSLQRFFVRGLLAGSVKG"
     gene            11158..12762
                     /gene="treS"
                     /locus_tag="PLC06_04680"
     CDS             11158..12762
                     /gene="treS"
                     /locus_tag="PLC06_04680"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HRO89772.1"
                     /translation="MSDKPWYQEAVFFELNIRACCDSDGDGNGDFRGAITKLDHIQSL
                     GVDCIWVQPMYPSPLKDDGYDVADYCGIHPDFGTLEDFKAFLDAAHARGLRVVTDLVM
                     NHCSEEHPWFQSARSDRNSPYRDYFVWSDTPDKYTGTRIIFLDVEPSNWTYDEVAGQY
                     FWHRFYASQPDLNYDNPAVHEEMFNIVRFWLDMGIDGFRADAVPYLYEREGTNCENLP
                     ETHAFLKKVRHLIDEEYPDRVLIAEANQWPEDLLPYFGDGDEFHVCFHFPIMPRLFMA
                     MKSGDKTPIIDILARTPAIPPGAQWMTFLRNHDELTLEMVTPEERQWMWEQYAPNPRA
                     RLNLGIRRRLAPLLDNDKRRWLALNAMFLSLPGTPIFYYGDEIGMGDNLALEDRYGVR
                     TPMQWSADKNAGFSTADHTYLPVIDDAVYGYQRVNVAAQEQDADSYLAWTRYLINTRQ
                     GQPALRAGVLEWVETGDEAVLAYRRTESGNRVLCVFNLTDKARPSGITPQEDVRDLLD
                     RNGRVHPAGVEVQLAPYGALWLLESA"
     gene            12883..13788
                     /locus_tag="PLC06_04685"
     CDS             12883..13788
                     /locus_tag="PLC06_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRO89773.1"
                     /translation="MTSPYVGGIEAGGTKFVCAVGTGPDDMRAEARFPTTTPEETVAR
                     AVAFFREQEKAHGRPAAIGIASFGPLDPNPASPTFGYITSTPKPRWANVDLAGAIRSA
                     LGVPVGFDTDVNIAGLGEWRWGAAQGLSNFIYLTVGTGIGGGVLIDGKPVHGLVHPEL
                     GHILIPHDRAADPYEGFCPYHGDCLEGMAAGPAIGQRWGRPAVELPPDHPAWDLEAHY
                     LALALQMFICTLSPERIVLGGGVMEQMQLFPLVRRKVIACLNGYIQSPSILSHIDDYI
                     VPPGLGNRAGVLGAFAQAQQVAAGQ"
BASE COUNT         2798 a         4017 c         4231 g         2911 t
ORIGIN      
        1 gggtcggtca ggacgctggg cgacacggcg acacgataat cccagcgatc gacggccagg
       61 atcgcgccgt tccgatcgta aattaccccg cgctcgggtt gatcggtgac cggcagctcc
      121 ttgatcagga cttttttgaa ttcctcgcct tgtgtgagtt gaaacgcgaa gagacggccg
      181 acgatgacca gaccggctcc ggcaataacc agggcaatca cccacatgcg tttgatctgg
      241 ctattgttca ttcgcctctc ctcgagtcag atcgatgccc agatctctga ctgccagaac
      301 aatggcctcc cacatggagg ccggtggttc gggctgtacg agcgggcctg tcggctcggc
      361 gcgctccacg gtgggttcgg tcggatagtt cggaatgaca atatattcga tatcgcccgg
      421 cgctgcggga acgaatccca gccgtcgcgc ctcggcctcc agtcgatcca gcgcctgagc
      481 ctcggcgatc tgtcgttcca gttcggcgtt ggtgcgcttc acctcgtcga gatcggcctg
      541 aacccgctga acgtggcggc cgatggccgc gattttgctc gcctgattca gataaatcac
      601 gcccagcaac gcgcccagga tgaggatcac cgcccagccg gtggccgcct gcgcctgggt
      661 aagcaaaccc aaacggcgag ccaactcgcg ggcctgacga ggacgacgtt tttcctcggg
      721 cgttgccatc attctgaatc ctcattgtca ctcttcgccg cgacgcgcaa tcgagcgctt
      781 cgactgcgcg gattggcggc gatctcgctt tctgtcgcct gaacggcctt tcgcgtcacc
      841 agttttaact ccggccgagc gccacaagtg cacaccggtt gctccggagg acacacacac
      901 gctcgcgcgg cgtcacggaa gaaatttttc accagccggt cttccagtga atggaagctg
      961 atcacggcca atcggccacc gggcttcaat agcctggtag cggcggtcag accgcgctct
     1021 acctcattga gttcgccgtt gacggcgatg cgcaacgcct ggaacacctg tgtggccgga
     1081 tggcgacccg gtcgcccgcg ccggccgatt tcgttctcga tcagatccgc cagttcggtc
     1141 gtcgtttgaa gaggccgatt ggcgatgatg agacgggcga tacgccggct atgcgtttcc
     1201 tcgccgtagc gccggaaaat atcagccagt tcttccgcgg ttaagttgtt aatcaaatca
     1261 gcggccgtcc ccccttgtcg gggatcgaac cgcatgtcca gcgggccttc ttttagaaag
     1321 gagaacccgc gattggcgtc ttctagctga cgagacgata gcccgaggtc gaggaggatt
     1381 ccgtcgacag cagcgaagcc gtgctcgggc gcaattgttc ccatgtcggc atagctggcg
     1441 acgacagggg tgagtcgatt accgcaggag tcgagccgtc gagtcgcgaa ctgcatggcc
     1501 tccgggtcgc gatcaaaagc aagcaagcgg ccgttcggcg cgatgaggtc aaggatggct
     1561 gaggcatggc cgccggcgcc cagagtcccg tcgatgaaca agtcgcccgg ttgcgggtcg
     1621 aggtaagcca aaacttcccg taaaaggacg ggaagatgac catactctcc tgttcgttca
     1681 gcatcatcga ccatttcata tcactaaaac catcgtcgcc atgagggcga ccgaaccgcg
     1741 gttgccctca tcagataccg aggttctccc aatgttgctc gtcgctttcg gcggcttcgc
     1801 gaacgggctc ccaggcatcg ctgttccaga tttccagatg atccagcatg ccggtgatca
     1861 ccacgtcgct gtcaatgttg gcgaactcgc gcagataggg gggcaagagc actcggccct
     1921 ggcgatcggg cacaaggtca atcgcgccgg agaagacacg ccggcgaaat tcgcgagcgc
     1981 tcgggtcgct ccatggcaag ctcttgacgc gggtggccaa ctcctcccag ccttccattg
     2041 agaaagccat gaggttgcga tcaaagccac gcgtgaccac catgccggcc gcgagcaagc
     2101 cgcggtattt ggccgggatg gtcaatcgcc ccttgtcgtc aatcgtgtgc cgatactcgc
     2161 ctaaaaacat tttatcctaa atagaacaac tgttctgtat tgtcccattc tggccgggaa
     2221 aatcagggtt gttgcgccaa tgcgccccac attgccccac tttttcccac aattggccat
     2281 agtatagcgc actcgttcca caattgaaag accgagattt gaagttttaa ggttcgaaat
     2341 gacaaaactc atatacagat atttgttgta gtatcccgca tgtggatttg tttgtctagc
     2401 gccctttggt aaaaaaatcc cgatatcttg cgacaaatca cctagagatc aatgttgggg
     2461 gtagcaaaag cggcgggagt gccggaacgc cgcggcccag gcaatgatct gtgcctggat
     2521 tgccgtttac gagggttatg aaactcagga gtggaggaaa ttaattggct gtctcttccg
     2581 gctcgtcgcc gcgcatcgct tccagcaatg tttccacgcg gtcatgacga tagagattca
     2641 acaacatggc tcgcactcgg gattcggtgt cggaaccttc cagctcctcc cagtcaatcc
     2701 ccaggtcggc ggccaactgc ttcagctctt ccgggctgaa gttggccagc aggtaatggc
     2761 gcaggacggt gcgcggggaa tcagccgaca gccgctccag tcggggcagg tgttcgggcg
     2821 gcggcggagt cagcacgcgc tcgacggcca gcgctacggt cggagcattg ccgtcaagtt
     2881 cggccgcatc catgaccgct acgatcaatt catccaggtt ccgcccgccg gtggcgatat
     2941 cggcctcgtt gatgttgaga tcgaacatca gatcgctcag atcggcgcgg ctgaagcgtt
     3001 ggcgcatctc ttcatagagc atttgccgat cgaatccggc ctcggcgtat tgatccacat
     3061 gatccggcgg caatggcggg gggagctttg attttgacgg aacgggtgcg gcggccgtgg
     3121 ctgcggcgat gacaggagcc ggggcagttg ttctggcggc cgaggcgatc atgggggtgg
     3181 ccgtagctgg ggtcatgtcc ctggccagca acggccgcac gacaccccag aaccaggcgg
     3241 ccgaaccggc cagcagcacg ccgctgatga cgtagaaaaa gaatcccagc tcggccagca
     3301 gttcctcacg attgaatgtg ccgaggagcg tatccagcag gacggccgtt gtcaccgtga
     3361 gcaggccaat accccaggcg acgttcggct tgcggacgaa catcgccagg aaaaacagga
     3421 cgaccagtag tttgaggata accagtacca tcggcgaatg attgtatgcc catgcccgtt
     3481 cgaaggcaac caccctatgg taaaatctta agcagcttgc gacatattgg tctgctttac
     3541 gtataataag caggttgagt taatcaattg agaaatagat tccctatcgg gacgggctgg
     3601 aaacagtggc aaatacaaat aaaccaagcg gcgaagaaat aaaggatatc ggtttctggg
     3661 gcgaggtgtg gcagcaaatc cgccttgtct tttatctcat caaggatcgg gaagtcccga
     3721 tctatttgaa ggccttgccg tttctgggcg tggcctacat gcttttcccc attgacatca
     3781 tcaccgattt tgtgcccgtg ttgggacagc tcgatgactt gactctgctg ctcatcggcg
     3841 ggaaggtctt tatcgagatg tctcccccgc aggtcgtcgc ccgttacctg gatcagatga
     3901 aggccgcccg cacagccacc atcatcgagg gcgaggtgag cgacgcggcc gatgaccctc
     3961 tcgcccacat gaccaaaggt attaagctaa tcgagggtga gttgtccgag gccgcttccc
     4021 ctattgagga aggcgaaccc gccgccaaaa agagctggtt ccgccgctag ggaagatagc
     4081 ccagctggcg cagcagccag tcaccgccaa taaaatacat aaagagggtc gtgctgcagc
     4141 agcaagcgag cagcgcgatc atacacccgg tcgcgatcag aatgatccgg ccgggtttgt
     4201 ttttctgagg cgcgggcatg accggcattg gcccaggatg atgctctata tgcgccggtt
     4261 cggtcaaaac aggatgctcg gccgtcacgg gcgatgattc agaagacaca acgcgctctt
     4321 cgacgacagg cggctcgtat tcgtcgccat tcgttgcgcc gatgtgccct aactcatcga
     4381 gtgctacgac gccctcgcct ccattggata cctcggtttc gctgacgaga gatgtcacgg
     4441 ccgccggata ctcatcgatc gccgtctcga tggcatgttc cagataggtg gcagatagac
     4501 gcgaacccag cagtatgata tcgccatggg caagggcaat gggttcagcg ccgacccgct
     4561 ctccgttgac atacgtgccc gaatcactgc ccaggtcggc gatccgatag gatgtgtcgt
     4621 cgcgcgtcag caaggcatgg cgataggcga tatcgggatc gttgatgacg atatcggcca
     4681 agggatagcg gccgatggtg atactcggcg cgaccaactg atactcctgg ccggcattgg
     4741 ggcctgactg gatcaacaat cgatgggatg tgtcgttcat cgtttcctcc gtatgcgcct
     4801 cagcgccgcg caaagaggcg cggcagatcg cgcgtttccg taatattaag cgcaaatacg
     4861 caaatcgtgt aggctgcgat gcccgcaagc ccgccgatag ccaccggccc caggcgagcg
     4921 atggtcggcg agacagtcca tcccaccgcc agttgcgcgg cgccgaaggc cgcaaagccg
     4981 gtcaggatcg ccgctatcag ggatttggcc aggcttcgca ggacggggtc gctcgcgaat
     5041 ccgcctagct gtcgccgcaa aacccagagc atgatgaaag tatggacgac atgcttcacg
     5101 gcgtcggcca ccatcaggct gagcaggccc aacggccgca gcaggagcag ggcggtcagg
     5161 gagtagatca ggatgctgat gaaccccacc agagcggggc gccaggtgtc tttccgcgcg
     5221 taggaagcga agacgagcat ctgatcggcg gcggcaaagg gcatcccgaa cagatagatg
     5281 cgcagcacca gggcggttgt cgctgtgtcg agcgccgtga agcggccgtg ctcgaacaac
     5341 aggccgatga tgaccggcgc cagagcgaag aggccggtcg tcgccggcag gatgagcgcc
     5401 agcaccaatc gcaggccgtc ggccagcgtt tgcttgaatt gggccagatc ggccactgcc
     5461 tgtcgcgaca gcgtcggcag ggtggcgatc gacagcgccg tgacgaccag ccccagcggg
     5521 aactgataga gcgtcgtggc gtagttcatg tagttgaggc tctggtcgcc ggttcgaatg
     5581 gccaggttgt agctcagcat gatcgcgagt tgattgacca ccagcccggc cagaatgggg
     5641 atgtagagtt tgatgatgcg acggatggcg ggatgttgaa aatcaagccg ccagcgcagg
     5701 cgggcgtcgc gcagggcggg gagttgcagc agcacctgaa gcaatgagcc gagcaggagg
     5761 ccccagacca ggctgcgaat atcggtcggg ttgagcaagg ccacgatcac gatcgtgccg
     5821 ttgaaagccg caccgacgaa ggcgggcagg gtgaaccgct tgagcgcgta gagagcgcca
     5881 gtcagaacgc tggcgatgcc gaggaagaac acggccggcg tcgtcatgcg gatgagcgtc
     5941 acagtgaagt ccgacagatc ggccgcagcg aagttggccg cgccgaccag ccaggctacc
     6001 tgtggagcga gcagttcgac aacggcgatg acccccaaca gcaccaatgt cgccgcgctg
     6061 agcaccatgc tcagaacctg ccaaagctcg tcgcgctttt ccttatcggc gtagtcgctg
     6121 aagaccggca cgagcgagga gttgaccatg ccgccgatga tgaggtcaaa caggctcgtc
     6181 ggcacatagg cggcggcctg gaacgcggcc aggagcggcg acgtgccgaa cagattggcc
     6241 ttgaccatct cgcgagccag acccagcacg cgactggcga cattgcccgc agccagcacg
     6301 gccgcggcgc ggacgatgcc gctttcgtgg gacgtttgtg gcctgtcgcc cgttgcctgc
     6361 tgcctgtact cttcggacag aaaggcgtcg ccatctattt cggccggcac cggcaaccct
     6421 tccggcgtcg ttggatcggg tatctgctcc atgtgggaca agtctagccc aatgggtgag
     6481 gggcgcaatg ggcggaaaat ttgcgcgcaa gctacgattg atagtcgatt ggccgagctg
     6541 ctcccattcg ctacattgtc cggcggccgt tataatctcc tctgctattt cctgttgctc
     6601 accacacaga acccgggcta ataaaaagct cgataccgga ggccttcatg tacgacaaag
     6661 ccaaactcgc cgaactagcc gaagcccgcg accgttggga ggagacgacc cttcaacaaa
     6721 cgctggcccg cgccccggag cgcaaggagc agttcgtcac cacctccggc gaaccgattc
     6781 gccgcctcta tacgccgctc gacgtagccg accttgatta tttgggcgat ctcggcaacc
     6841 ccggtgatta tccctatacc cgtggtgttc actcgacgct ctatcgcggc aagccatgga
     6901 cgatgcgcat gttcgccgga ttcggcagcg cggaggagac caacgctcgc tacaagtatt
     6961 tgttgagcca gggcaacatg ggcctctcgg tggctttcga tctgccgaca ctgatgggct
     7021 atgacaccga cgcgccgcag tcattgggcg agttcggcaa gtgcggcgtg gccatcagca
     7081 gcctggcgga tatggagatt ttgttcgacg gtatcccgct cgaccaggta acgaccagca
     7141 tgaccatcaa cagcccggcg gccatcatct gggccatgta catcgccgcg gccgagaagc
     7201 aaggcgtccc ccaggccaaa ctgggcggca cgctgcagaa cgacatcctg aaggagtaca
     7261 tcgcccagaa ggaatacatc ttcccgcctg agccgtcgat gcggctggtt atcgacacca
     7321 tcgaatatgg cgcgcgccac atgcccttgt ggaacactat ctctatcagc ggctatcaca
     7381 tccgcgaggc aggatcgacg gccgcacagg agctggcctt cacattgggc gatgggctgg
     7441 aatatgtgcg ctgggcgatt gctcggggta tggatgtcga tgagttcgcg ccacgcctga
     7501 gcttcttctt caacagccac aatgacttct tcgaggagat cgccaagttc cgcgccgctc
     7561 gccgtatctg ggcacgggag atgcgcgaaa cttttggcgc gaagaacccg cgctcgtggc
     7621 tttgccgctt tcacacacag acggccgggg tcagcctgac ggcacagcag ccggaaacca
     7681 acgtcgtgcg cgtcgccatc caggcgctgg cggccgtgct gggcggcacg cagagtctgc
     7741 atacgaacag catggacgaa gcgctggcgc tgccgagcga ggaggcggtc actgtggccc
     7801 tgcgcacgca gcagatcatc gccgaagaga gtggcgtcgt gaacacagtc gatccattgg
     7861 ccggttcctt ctttgtagag cacgagacga acaagatcga ggcccaggtc tacgactact
     7921 ggcgacaagt ggacgagttg ggaggcgtgc tgccggccat cgagcagggc ttctaccagt
     7981 cggagatatc ggttgcagcc tatcgctatc agcgcgagat cgatcgcaac gagcgctata
     8041 tcgttggcgt caatgcctat gccgatccgg acgaaaagct gcgcatcccc attcttgcga
     8101 tggaccccaa tggctatgac cgccagatcg cgcgcctgaa gcgcctgcgc gaggagcggg
     8161 acaacgagcg ggtgagcgag acattggcgg ccttgcgatc ggccgcggcc ggaaccgaga
     8221 atacgatgcc ctacatcctt gacgccgttc gcgcctacgc cacgctgggc gagatcaccg
     8281 acgtgttccg cgaggtgttt gggagatacg aagagccgac gtggatttga gtgtgggcag
     8341 gtgacacacg gcagatgacg atgatcatcg gccgcttgtg gcatcaaccg cgcgacgcac
     8401 cctttttgac aacccgtctg agtaactata tagtctgcga aaaggaactg gccccggacg
     8461 atcgctcgat gcaagctggt tctcacggga gagcgtagtt gctgtgacca tcaaacaagt
     8521 tggcggaaga gcggatgctg atctttttta tccacccggc aattacactg cctatgcgag
     8581 cggcgtcgct gatccggata ccgccctctc cgagatcgac gcctcatggc taactgagtg
     8641 atctgtattt gcaagaaact gccggccttt ttggggtcgg cagtttctca tttataatcc
     8701 cgacggccgt tttatcaact gatcaataag gaggttttgg cgtatgcaat ctgaatctgc
     8761 ccaggaacaa tcgggattgg tcgcccggct cctgaacctt gccttacccg ttgctgccct
     8821 gatcatcgcc ctggttgcga tgtatctggg tttcgacttc cttcgggccg gaagcgcgcc
     8881 aaagtggctg atcgtgctgg tggccatcgt ttggggcgtc ggcggcgtcg ctgcgcttta
     8941 ttggatcctt aactggatcg tcgagcgcat gaaccttaag tggcgggggc ggcttcaacc
     9001 ctttgttttt gtcggcccgg ctatcgtcat gttgatgtgg ttcctggccg tgcctgttct
     9061 gcgaaccttc tggctgagcc tgttcaatcg ggatggcccg ccgattggtt ggctgaactg
     9121 gagcgatata ggtggcagct ttgcctcatt gagcgggcgc ttcgtcggcc tcgataacta
     9181 tgtcgcggtt ttcaccgatc gcctgatgag agaagcgttc cgcaacaaca tcatgtggat
     9241 cgtctttggc gcgacgctga ccgtcattgt cggcttgctg atcgccgtcc tcgcggatcg
     9301 aagcagcttt gagcgcgtga gcaaatcggc catcttcctg ccgatggcga tttccttcgt
     9361 cggcgcgggc gtgatctgga attttatcta tgaggtccgg ccggtcgatg ttccccagat
     9421 tggtctcctg aatgcggttg tcgtcgggct gggaggtaaa ccgcaaccct ggccggcctg
     9481 gacggcgatc agcccctgga ataatctgtt ccttatcgtc atcgtcatct ggctacaggt
     9541 cggttatgcg atgaccttgt tctcggccgc cctcaagggc atcccggctg acctgctgga
     9601 agccggtcgc gttgacggtg ccaatgagat acagattttc ttcaggatca tggtccccta
     9661 catcatgggc acgatcatca ccgtcagtac gactgtcgtc atctttacac tcaaggtatt
     9721 cgacgttgta tgggtgatga ccggcggaca atttggaacg gaagtcatcg cgacccagtt
     9781 ctacaggcaa tcattcacgg cccgtaactc cggttatggt tcggcgatcg ccatcgtgct
     9841 gctcatcgcc atcgttccgg tgatgatcta taacctgaag caattccggg aacaggaggc
     9901 gttctaacat gggaagcatt caaaatcagc gacgatgggg ttccttgctg gtcaatatcg
     9961 gcttgctgtt attggttctg ttctggacca ttccgacgtt tggtctgcta gtctccagct
    10021 tccgcacacg agagtctatc caggcttcgg gctggtggaa tgtcttcccc caccgcgctt
    10081 atgagactgt agcgacactc agcccggctg aaatggggtt ggatccgacc ggccccatgg
    10141 tgatcgaagg ggtggagacg accttcgagg agatgcgcaa aacgggtatt actactccgg
    10201 atggaacgtt ctatcgttgg gtgggtaatc gacgcatcgg ccgagtcgaa atccaggagc
    10261 aaagctggac ggtcgattgg gatttcaccc tggacaacta tcgccaggtc ctgagcggca
    10321 agaacttcga gtataaacaa ccggacggca ccatcaaggt tatcccgggg gataatttca
    10381 tcagcgcctt ccttaatagt ctggcggtag cggttccggc cacggtcatc ccgatcctgg
    10441 ttgccgcttt tgccgcttat gggttcgcgt ggatgaattt ccccggacga aggatgttgt
    10501 tcatcatggt ggtggcgttg ctggtcgttc cgctgcagct ggccctggtg cctatcttgc
    10561 gcgacttccg atcattgcag atcaacggca ccttcatggc catgtggctg gcccataccg
    10621 gctttggctt gcctttggcc acctacctgc tgtttaacta catcagcacg ctgccgcggg
    10681 agacgctgga atcggcgttc atcgacggcg catcccattt tgtagtgttc cgcaagctga
    10741 tcctgccgct gtcggtgccg gcgttggcgt cgttcgctat tttccagttc ctctgggtct
    10801 ggaatgatta tctggtggcc cttatcttcc tcggcggcag cccggagacg aaggtcatca
    10861 cgcagcgcct ggctgacatc gttggttcac gcggcaacga ctggcatctg ttaacggccg
    10921 gagcgttcct caccatgctg ctgccgatga tcgttttctt cagcttgcag cgcttctttg
    10981 tgcgtggctt gctggccggg tctgtgaagg gttaatcttt aatccgcttg caagcggatc
    11041 aaagacagtg aaaaataatg agcacggggt cgtcggagag gcttcgtgct catttcatgt
    11101 gaccctattc tcgcgagcgg caggtcatat ttgtcccaat ttgtcaggag caagtgaatg
    11161 tccgataaac cctggtatca agaagcagta ttcttcgagc taaacattcg cgcgtgttgc
    11221 gattccgatg gcgacggcaa cggcgatttt cggggagcca tcaccaaact ggatcatatc
    11281 caatcgctgg gcgtcgattg catctgggtg cagccgatgt acccctcgcc tctaaaggat
    11341 gacgggtatg acgtggccga ttattgcggc atccaccccg atttcggcac gctggaggat
    11401 ttcaaggcgt tcctggatgc ggctcatgcg cgcggccttc gcgtggtgac cgatctggtg
    11461 atgaaccatt gttccgaaga acacccgtgg tttcagagcg cccggtctga ccgcaactcc
    11521 ccctaccggg attacttcgt gtggagcgac acgcccgaca aatataccgg gacgcggatt
    11581 atcttcctgg acgtagagcc gtcgaactgg acctatgatg aggtcgccgg tcaatacttc
    11641 tggcatcgtt tctatgccag ccagcccgat ctgaactacg acaatcccgc ggtccatgag
    11701 gagatgttca acatcgtccg gttctggctg gatatgggta tcgatggctt tcgcgccgat
    11761 gccgttccct atctctacga gcgggaagga accaattgcg agaacttgcc ggagacgcat
    11821 gcgttcctga agaaggtacg ccatttgatc gacgaagaat atccggatcg agtcctgatc
    11881 gccgaggcca accagtggcc agaggatttg ctgccctatt ttggtgacgg tgatgagttc
    11941 catgtctgct tccacttccc catcatgccg cgtctgttta tggccatgaa gagcggcgac
    12001 aagacgccga tcatcgatat cctggctcgt acgcccgcca ttccccccgg cgcgcaatgg
    12061 atgaccttct tgcgcaacca tgacgaactg acgctggaaa tggtgacgcc ggaagagcgc
    12121 cagtggatgt gggaacaata cgcccctaat ccgcgcgcgc gactcaacct gggcatccgt
    12181 cggagactgg ctccgctgct cgataatgac aagcgacgct ggctggcgct caacgccatg
    12241 ttcctgtcgc tgccgggcac gcctatcttt tattacggcg acgagatcgg catgggcgac
    12301 aatctggcgt tggaagaccg ctatggtgtg cgcacgccga tgcaatggtc ggccgacaag
    12361 aacgccggct tctcgacggc cgatcacacc tacctgccgg tcatcgacga cgcggtatac
    12421 ggctatcaac gggtgaatgt ggccgcccag gaacaggatg ccgattctta cctggcctgg
    12481 acacgctatc tcatcaatac gcggcagggt cagcccgcac tgagggccgg agtgctggag
    12541 tgggtcgaga cgggcgacga ggccgtgctc gcctaccgtc gcaccgagag cggtaaccgt
    12601 gtcttgtgtg tgttcaacct gacggataaa gcgcggccgt cgggcattac accccaggag
    12661 gatgtccgtg acttgcttga ccggaatggt cgcgttcacc cggcaggcgt tgaggttcag
    12721 ctcgccccct atggcgcctt gtggctgctg gagagcgcct gagaaagccg ataacgaccc
    12781 accggcaatt actcttgcgg gaggggccgt cccgatgatc tttgtcggtg ttgccggcgg
    12841 gctgttcgac aatggattgc ttgaagcgag ggagatagca cgatgacaag cccatatgta
    12901 ggtggaatcg aggcgggcgg cacaaagttt gtttgtgctg tgggtacggg gcctgacgac
    12961 atgcgcgccg aggcacggtt tccaacaacg acacctgagg agacggtcgc ccgcgcggta
    13021 gccttcttcc gcgagcagga gaaggctcac ggccgcccgg cggctatcgg tatcgcctct
    13081 ttcgggccgc tcgatcccaa cccggcctcg ccgacgttcg gttacatcac ttcgactccc
    13141 aaaccgcgct gggccaatgt cgatctggcc ggggccattc gctcagcgct tggcgtgccg
    13201 gtcggcttcg ataccgacgt gaatatcgcc gggctgggtg aatggcgctg gggcgcggcg
    13261 cagggcctgt ccaacttcat ttatctgacc gtcggcacgg ggatcggcgg cggcgtgctc
    13321 atcgacggca agcccgttca tggcttggtg catccggagc tggggcacat cctgataccc
    13381 cacgaccggg cggccgatcc ttatgaaggt ttctgcccct atcatggcga ttgcctggag
    13441 gggatggctg ccgggccggc cattggccaa cgttggggac gtccggccgt tgaattgccg
    13501 cccgatcacc cggcgtggga ccttgaggct cattatctgg cgctggcgtt gcagatgttc
    13561 atctgtacgc tctcgccgga acggattgtc ctgggaggtg gggtgatgga gcagatgcag
    13621 cttttccctt tggtgcggcg caaggtgata gcctgcctga atggatatat tcaatcccca
    13681 tccatcctga gccatatcga cgattacatt gtgccaccgg gattagggaa ccgtgccggt
    13741 gtgctggggg catttgccca ggcgcagcag gtggcggccg ggcaataaaa aagcgcggga
    13801 gaatcgcctc tcgcgcttgc catcgagttt gtaggtttta atcgttgtga ccaccgatca
    13861 ggagtcgcac atgataacag ctgctcgaaa aagaatatct actcttgata ttttctctga
    13921 gcggcgacag gtgcaagccc cttttccttc ctggctt
//
LOCUS       DAOMQG010000050        13585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1944_length_13585_cov_6.958389, whole genome shotgun sequence.
ACCESSION   DAOMQG010000050 DAOMQG010000000
VERSION     DAOMQG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_13585_cov_6.958389"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..788
                     /locus_tag="PLC06_04690"
     CDS             297..788
                     /locus_tag="PLC06_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89774.1"
                     /translation="MNPTILVITGDAAWTRRAIHLAAAMARDAAGELRIIHLLPVARL
                     EDLGANAHEELLPFDRYQALADWAATAESYGVAVHVELFEYSDYAGALLCAAEQTGPR
                     AIFAPPPGGRPAFVARLREKWLRRRLGRPYFTLGPDDSPTAWSETPAETRPTATATRE
                     VRI"
     gene            826..2841
                     /locus_tag="PLC06_04695"
     CDS             826..2841
                     /locus_tag="PLC06_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRO89775.1"
                     /translation="MSHTIETPSGEAPEKGKRFNLSDLLIGRPLETRTLAHQVVSNKV
                     GLAVFASDALSSTAYATEEILVILALTGTMAYLGLSIPIAIAIGILLVIVTISYRQTI
                     HAYPNGGGAYIVARDNLGEVAAQVAGAALLTDYILTVAVSISSGVAQIVSAFPALAPW
                     RVAMAVGIIAFITLINLRGVKESGTVFSIPTYFFLVMMALTLGVGFYRWATGTLGMVT
                     GVEFAVEAAAPLTMFLVLRAFSSGSAALTGIEAISNGITAFREPRSQNASRTLVVMST
                     LLVTMFLGITALSRHIGAIPSHEETIISQLARTIFGDGNILYLLVIAGTALILLMAAN
                     TSYADFPRLAALHAGDGFLPRQLTYRGGRLVFSWGIMTLAIAASVLVIVMGASTTALI
                     PLYAIGVFLSFTISQTGMVVRLRKIGKLQPGEEVQGLETVMKYDPHWRIHMVISAFGA
                     LCTFMVMMIFAVTKFSHGAWFILLLIPVLVFIFFRIHHHYKDVAQALSLENAHVKEIE
                     TNRVITVLLVDSVHMGTRQMVRFAKSMGNPWHAVHIGVNHEKSSTAQARWDKYIGEGE
                     LVIIDSPYRHLVAPIREYIIGLLKDNPDAIIHIVMGHLAMDSLMTQALHQNSSLILNM
                     GLTGLERVVVTIVPLQVRQNGGEVNRNMLTGDDLKRARVERKTTSGE"
     gene            complement(3167..3541)
                     /locus_tag="PLC06_04700"
     CDS             complement(3167..3541)
                     /locus_tag="PLC06_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO89776.1"
                     /translation="MILCDTNILIEFYKNNPEINAVLRSAGHTNLAVSIITQAELYFG
                     ALNKNELESIQRHLALITCIQVSESISHRFIRLMESYSLSHKLAIPDALIAATAIENE
                     LALYTLNRRDFRFIQNLQLFEP"
     gene            complement(3538..3765)
                     /locus_tag="PLC06_04705"
     CDS             complement(3538..3765)
                     /locus_tag="PLC06_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89777.1"
                     /translation="MEQITILVRDKKKARSLLDLLRSLDFVSVLDVDEVDSSAEMMEH
                     ERQFFAAAGLWEGREVTTESLRSIAWPQDNS"
     gene            complement(3980..4159)
                     /locus_tag="PLC06_04710"
     CDS             complement(3980..4159)
                     /locus_tag="PLC06_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89778.1"
                     /translation="MVALVQTMLDLHRRHAAEANPHVKTLLQRQIEMTDKQIDALVYA
                     LYGLTAEEIAIVEGN"
     gene            complement(4224..4913)
                     /locus_tag="PLC06_04715"
     CDS             complement(4224..4913)
                     /locus_tag="PLC06_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89779.1"
                     /translation="MKLVILIISLFLLTGCSPAAPEVITEQIEVTRLVETEVTHIVEK
                     PVEVTRLVEVEVTRVVAPTATPRPPATATPKAPEIGTRDNPVPVLAPADVVITVNSGR
                     QTATFTVLDIARGDEALQMARAANRYNDPPTEGNEFLLALIEIAYHEGDGVLELGRHA
                     TALVSNNRIIDFGDMAFEVPCCLKPEFDFQLFPDAIANGWLPLMVPIDDVAPLLLIGD
                     PIGGVYFSLTE"
     gene            5290..6354
                     /locus_tag="PLC06_04720"
     CDS             5290..6354
                     /locus_tag="PLC06_04720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRO89780.1"
                     /translation="MPPFELGLYSFGELTPDPRTGRLISPEQRIRYLLEEIELADQLG
                     LDVFALGEHHRPDYVVSAPAVVLAAAAARTKRIRLSSAVSIISSDDPVRVFQQFATLD
                     LISGGRAEIMAGRGSFIESFPLFGYDLNDYGELFTEKLELLLALRQAEQITWSGNHRA
                     ALTGQGVYPRPVQDPLPVWIAVGGTPESAARAGLLGLPLALAIIGGMPERFAPFADLY
                     RRALAHAGHDPAATPFSINSHGFIADDSQTAADLSFPPFKLVMDKIGRERGWPPMSRA
                     QFDESRMLRGANFIGSPEEIVEKILFQHDIFRHQRLMLQFSVGTMAHADIMRSIELYG
                     TRVAPAAREEIGRRMAQQSR"
     gene            6592..9162
                     /locus_tag="PLC06_04725"
     CDS             6592..9162
                     /locus_tag="PLC06_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89781.1"
                     /translation="MFPFNRFGRVSLVFLSVLLFAGACRQFSGGPNTGNGDPAAVFAP
                     DNNSGVDDTVDGAANNSAGAYPSKVSVLPGESLDFHISNGYSGKYTLSIYREGATRTL
                     MGTVPNVSTGNYSCAGGYDNGCSWPVGATFKIPNSWPSGVYTVDIPRNSGNPMRTLFY
                     VRPAQPKARILFLSSVNTIQAYNDYGGGSLYGFDNTVKSERVSFDRPYANGVGLYDRW
                     ESYFVTWAEQAGYGMDYAATYDLDFVPRLLDGYDLVIIAGHSEYWTWAMRQRLRAYID
                     GGGRFMNLSGNTMWWQVRFEDNGRTMVGYKSWRKDPEKSAELSTDVNWDRPIFDSSFT
                     ITGLHWPYGGYPGGTGDGYYVIKANHWIYQGTGLTENTLFGKGPTVDTSIHDKESDGL
                     AFNCAADGSTLLGPVAGTGTPANFTILGLTRVHSKQRDLDGVAMMGLYTTPAGGAVFS
                     AGTTGWVLGLGQPAVDRITRNVMDRFLAGSFPQEPAAPDSGHLFADRFNCTDLGRGRF
                     LATLNDAARLNYVITEKATTARLTAACGADGAGLQLPAGSTGVRFVAGLGPNWRSNFA
                     IETQVDLNLAGFSIDKGATLTLMTHYADNRVDAPVTVAALQLGNVDNVLSARYQPAGG
                     PEMPWVRVPANGFFRVNTTWDGQRDKVGLTIDGSGYERQVELGNSPLPNRADHGILQK
                     QGASGGTICMDNLIYDGRPFGVEPTPTPTPTATPTPTATPTRTPTPTPTATPTPTATP
                     TPSGDGIHIGDLDGGSENMPNSRWRATVTITVHDANHVPVARARVRGKWSGGASGSGS
                     CMTNAGGTCSLDKIVLSQNSSATFTVTKLSKTGATYRSTASHDVDGGTNGTVIVVNRP
                     "
     gene            complement(9265..>9610)
                     /locus_tag="PLC06_04730"
     CDS             complement(9265..>9610)
                     /locus_tag="PLC06_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89782.1"
                     /translation="DAYQALLALGDGGPTATPTKTATPSPTPTKTPTPLPTATDGPSP
                     TPLPTTEATVTPSPMPSPTTEATPSPTIEATPSPTSEATPSPTIEATPSPTATLPPIG
                     DFALFLPVVIDQ"
     assembly_gap    9611..9710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9711..11231)
                     /locus_tag="PLC06_04735"
     CDS             complement(<9711..11231)
                     /locus_tag="PLC06_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRO89783.1"
                     /translation="MDHNRKPTMRHGLLAIAFLASLVVALWLLPGGRTAARAIEPSPA
                     RAGQPADRPADQLMIRFAPIAGRAPDAAARQQLLDRMNAAAGTTLTFVRPMSGDAFVY
                     RLPKPLAVDQVAAIARDLAALPEIDYAEPDIIMRHTGIRVVSPLTGTPRAADQSPNDP
                     GFADQWHYRYTPGDEEGANLEPAWAITTGSADIVVAVLDTGITGHADLAGRTVPGYDF
                     ITNPATANDGDGRDPDPSDPGDWNEAGQCDGDPFSPSSWHGTHVAGTIGAASNNGDDV
                     AGVNWQAKILPVRVLGRCGGYPSDIIDAVRWAAGLAVPGVPANATPARVLNLSLGGSG
                     TCPASWQSAFNDVTAAGAVVVVAAGNSSKIADFYTPASCKGVITVAATDRYGYLAWYS
                     NYGSTVEISAPGGDTWYEEDGILSTLNSGTRGPAADSLAFYQGTSMAAPHVAGVAALL
                     LGEKPGLTPVQVSELLQDSARDFPDISDCTTASCGDGMLDAYQALLALGDGGPTATP"
     gene            11498..13120
                     /locus_tag="PLC06_04740"
     CDS             11498..13120
                     /locus_tag="PLC06_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRO89784.1"
                     /translation="MLGAALLIAAALLAGIGLLIYLPARAATPEKPNIVIVMSDDQDM
                     DSLPVMRKLMANPEGSWVNFTHAFSNTSICTPARATLLTGQYSHTNGILGNNWGADFN
                     NDNTLAVWLDDAGYKTALIGKFLHYQPGENGDERGQYDMPGWDLYSISYLTVDEHTQE
                     AVDFISDAKSPFFLWLAYRAPHKTADPPPRYEGIDVYIPPDRPNFNEKDMSGKPEYMR
                     KQPLLTPEEITGLRKERANSQRELLAVDDGIESVVNALKAKGELDNTMIIFVGDNGFS
                     WGSHRLYEKLCPYEECSMVPLFIRYPGTGENRIEEHFVSMVDLAATIADYAGVKPKLP
                     QDGRSFLPLLNGTAPWWDDTVLLEKVAGRNAHYAARSPGWTYVEYITGEKELYDLTAD
                     PYQLKNLAGKPGYAAQQAVMAQKLYYLLNPPAPTPTATPTVSPTPTVSPTPTGTPTAT
                     GTPSPTSTPSLTPTDGPSPTPTATETPSPTATDGPSPTPTATETATPTETPSPTATAG
                     PSPTPSPTATAAPPDRAIYLPIITGGAAVPVP"
BASE COUNT         2567 a         4327 c         4122 g         2469 t
ORIGIN      
        1 cactgaccca ctgacccact gacccactga cccactgacc cactgaccca ctgacccact
       61 gacccactga cccactgacc actgatttta cagattcttt acgcctttcc ccgttttccc
      121 tgacattttc ctgacggccg caggcctata ctgttcccgt aaggagcacg aggagcagcc
      181 gggcagggct tgtcgttgag cggtcgacac agccacccca tctcacaatc cccgcctggc
      241 ccgcttcgtc aacaacggcc ccctgccctg caaccatacc atcacgaggc aagcgtatga
      301 acccgacgat actggtcatc actggcgacg ccgcctggac gcggcgcgcc atccatctgg
      361 cggcggccat ggcccgcgac gcggccggcg agttgcgcat catccatttg ttgccggtgg
      421 cccgtctgga agacctgggg gctaatgcgc acgaggagct gcttccgttc gaccgctacc
      481 aggcgctggc cgattgggcg gccacggccg agagctacgg cgtggcggtt catgtggagc
      541 tatttgaata cagcgattac gccggcgcgc tgctctgcgc cgccgaacag acggggccgc
      601 gagcgatttt cgcgccgccg ccgggcggtc gcccggcgtt tgtcgcccgc ctccgcgaga
      661 agtggctgcg gcggcggctg gggcggccgt atttcaccct cggccccgac gacagcccca
      721 cagcctggag cgaaaccccg gccgaaacgc gccccacggc gacggccaca cgcgaagtga
      781 gaatataagc gagaaagagg cgagccttga ggcttgcgca catccatgag tcacacaatc
      841 gaaaccccat ctggcgaagc ccccgaaaag ggcaaacggt ttaatttatc cgacctgctc
      901 atcgggcggc cgctggaaac gcgcacgctg gcccatcagg tcgtcagcaa caaggtcggg
      961 ctggcggtgt tcgcctccga cgcgctctcc tccacggcct acgccaccga ggagattctg
     1021 gtcatcctgg ccctgacggg cacgatggcc tatctgggcc tgtcgatccc catcgccatc
     1081 gccatcggca tcctgctggt catcgtgacc atctcctatc ggcagaccat ccacgcctac
     1141 cccaatggcg gtggggccta catcgtcgcc cgcgacaatc tgggcgaggt ggcggcacag
     1201 gtggccggcg cggcgctgct gaccgactac atcctgaccg tcgccgtgtc catctcgtcg
     1261 ggcgtggccc agatcgtctc ggccttcccc gccctggcgc cgtggcgcgt ggccatggcc
     1321 gtgggcatca tcgccttcat caccctcatc aatctgcgcg gggtgaaaga gagcgggacg
     1381 gtgttctcca tcccgaccta tttcttcctg gtcatgatgg cgctgacgct gggcgtcggg
     1441 ttctatcgct gggccacggg cacgctgggc atggtgacgg gggtggagtt cgccgtcgag
     1501 gcggccgcgc cgctgaccat gttcctcgtc ctgcgcgcct tctccagcgg ttcggcggcg
     1561 ctgacgggca tcgaggccat ctccaacggc atcaccgcct tccgcgagcc gcgcagccaa
     1621 aacgcctcgc gcacgctggt ggtcatgagc acgctgctgg tgacgatgtt cctgggcatc
     1681 accgccctgt cgcgccacat cggggccatc ccgtcgcacg aggagacgat catctcccaa
     1741 ctggcccgga cgatcttcgg cgacggcaat attctctatc tgctggtcat cgccgggacg
     1801 gcgctcatcc tgctgatggc ggccaacacc agctacgccg acttcccgcg gctggcggcg
     1861 ctccatgccg gcgacggctt cctgccgcgc cagctcacct atcgcggcgg ccgtctggtc
     1921 ttctcgtggg gcatcatgac gctggcgatt gccgcctcgg tgctggtcat cgtcatgggc
     1981 gcgagtacga cggcgctcat cccgctctac gccatcggcg tgttcctcag cttcaccatc
     2041 tcgcagacgg gcatggtggt gcgcctgcgc aagatcggca aattgcagcc cggcgaggaa
     2101 gtgcaggggc tggagacggt gatgaagtac gacccgcatt ggcgcatcca catggtcatc
     2161 agcgccttcg gcgcgctctg caccttcatg gtcatgatga tcttcgccgt caccaagttc
     2221 agccacggcg cgtggttcat cctgctgctc atcccggtgc tggtcttcat cttcttccgc
     2281 attcaccatc attacaagga cgtggcccag gcgctgagtc tggagaacgc ccacgtcaag
     2341 gagatcgaga cgaatcgggt catcaccgtg ctgctggtgg acagcgtcca catgggcacg
     2401 cgccagatgg tgcgcttcgc caagtcgatg ggcaacccgt ggcacgcggt ccatatcggc
     2461 gtgaaccacg agaagtcgag caccgcccag gcccgctggg acaagtacat cggcgaaggg
     2521 gagctggtga tcatcgattc cccctatcgc catcttgtcg cgcccatccg cgagtacatc
     2581 atcgggctgc tgaaggacaa ccccgacgcg atcatccata tcgtgatggg ccatctggcg
     2641 atggattcgc taatgaccca ggcgctgcac cagaacagct cgctcatcct caacatgggg
     2701 ctgaccgggc tggagcgggt ggtcgtcacc atcgtgccgc tgcaagtgcg gcagaatggg
     2761 ggcgaggtga atcgtaatat gctgacgggg gatgatctga agcgggcgcg ggtggagagg
     2821 aaaacgacga gcggggagtg acgaattacg aattacgaat tacgaattac gaatgaagag
     2881 cgccgctggt cgggcgacgt tcttcgcttg tagttaacgg ctttagccgt gttcttttgg
     2941 ctgcctcccg tcggccgccg gggggggggt ggagtcgctg tcgacggccg aaagtgaccg
     3001 aatcgcgctg gaacaattag gaatatagtc ctctctaccc tcttcgctcg tagtcaaacg
     3061 cttcagcgcg gttccccttc tgcgcctctg cgcgaggaac gattttctat cgtagttcgt
     3121 agttcgtcac ctcgtcggcg gacaggagtc cgctcgtcac tcgaagttat ggctcgaaca
     3181 attgcagatt ctgaataaac cgaaagtctc ggcggttcag ggtatagagg gcaagctcat
     3241 tctcaatagc ggttgcggca atcagcgcgt caggaatcgc taacttgtgg ctgagggaat
     3301 agctttccat gagtcggatg aaacggtggg atatcgactc gctgacttga atgcaggtga
     3361 ttaatgccaa gtgccgctga atggactcca gttcgttctt gttcaaagcg ccgaagtata
     3421 gctcggcctg ggtaatgatg ctgacggcga ggttggtatg gccggcggag cgcaataccg
     3481 cgttaatttc ggggttattc ttgtagaact caatcagtat gttggtgtca cacaggatca
     3541 tgaattatcc tggggccacg ctatcgatcg caacgattcg gttgtgacct cccgaccttc
     3601 ccaaagaccg gcagcggcaa agaactgcct ttcatgctcc atcatctcgg ccgaagagtc
     3661 tacctcatca acgtcaagca cggatacgaa atcgagcgaa cgcaagagat ccagtaatga
     3721 tctggccttt ttcttgtctc gaacgaggat cgtgatttgt tccataatgg cgcacctctt
     3781 gtgtgtccac attcattata ccagaacctg gcaagtatcg gaacgattct ataagtgaat
     3841 aagtgacgag tggcgagtgg cgaatgacaa gagcgcgtct ttcgtacttt gtctacagac
     3901 cacagacgac agaccacaga ccacaaggag agcgcgcctc cttcgtacct cgtacctcgt
     3961 acttcgtagt tcgtacttcc taatttccct ccacaatcgc gatctcctcg gccgtcagcc
     4021 catacaacgc atagaccagc gcgtcgatct gcttgtcggt catctcaatc tgccgctgca
     4081 gcagggtctt cacatggggg ttggcttcgg ccgcgtggcg ccggtgcagg tcgagcatcg
     4141 tctggacgag ggcgaccatg cggtcgtggt tggacctttg cgtccctgta cctttgcgtt
     4201 aatttttgtc ggtagtcgat gtgctattca gtcagggaga agtaaacgcc gccaatgggg
     4261 tcgcctatga gcaggagcgg cgcgacgtca tcgattggta ccatgagcgg cagccagccg
     4321 ttggcgatgg catcagggaa tagctggaag tcgaactccg gcttaaggca acatggaacc
     4381 tcaaaagcca tatcgccgaa atcaatgatc cggttgttgc tcaccagcgc tgtggcgtgg
     4441 cgcccaagct ccagcactcc atcgccctca tggtaggcaa tttcgatcaa agccagcaga
     4501 aattcgttac cctccgtcgg cgggtcgttg tatctgttgg cggctctcgc catttgcagc
     4561 gcctcgtccc cacgggcgat atcgagcacg gtgaaagtcg cggtctgccg cccggagttg
     4621 acggtgatga caacatcagc gggcgcgagg accggcacgg gattatcgcg cgtgccgatt
     4681 tccggcgctt tcggcgtggc tgtagcgggt gggcgcggcg tggccgtcgg cgcgactacg
     4741 cgcgtcacct cgacctctac caagcgtgtc acttcaactg gcttctcgac gatgtgggtg
     4801 acttctgtct ccaccaggcg cgttacctca atctgctcgg tgatgacctc aggcgcggcc
     4861 ggtgaacaac cggtcagcaa gaacagacta ataatcagga taaccagctt cacaacatcc
     4921 tcctaatata ggaacaatac agtggattct cgcctttcag ctttcggcgc gattcagcgc
     4981 gatctcccta aaaacaaggg ccggaacctg attggccccg gccccgccac acgagaggtt
     5041 gcgaccggac cgacaaagct tgtatgcctt cactctacca tagggacgag gggaataacg
     5101 ccacagaatg gaccgccgac ggacgaccgc cgaccgcggc aagaaatcaa cgcaaaggcg
     5161 caaaggccct ctcttccctt gcatctttgc gcctttgcgc tacattcccc ccatcaacca
     5221 tttcgtaact cgtaactcgt aactcgtaat tcgtaattcg taattcgtaa ttcgtaattc
     5281 gtaattccca tgcccccctt cgaactaggc ctctactcct tcggcgagct gacgcccgac
     5341 ccgcgcaccg gccgcctcat cagccccgaa cagcgcatcc gctatctatt ggaagagatc
     5401 gagctggccg accagctggg cctcgatgtg ttcgcgctgg gcgagcacca ccggccggac
     5461 tacgttgtct cggccccggc ggtggtgctg gccgcggccg ccgcccgcac gaagcgcatc
     5521 cgcctcagca gcgccgtctc catcatcagc tccgacgacc cggtgcgcgt cttccagcag
     5581 ttcgccacac tcgacctcat cagcggcggc cgcgccgaga tcatggccgg gcgcggctcg
     5641 ttcatcgaat cgttccccct gttcggctac gacctcaacg actacggcga gctgttcacc
     5701 gagaagctgg agctgctgct ggccctgcgt caggcggagc agatcacctg gtcggggaac
     5761 caccgcgcag cgctgacggg ccagggcgtc tatccgcgcc cggtgcagga tccgctgccg
     5821 gtgtggatcg ccgtgggcgg cacgccggag tcggcggcgc gggcggggct gctggggctg
     5881 ccgctggcgc tggccatcat cggcggcatg ccggaacggt tcgccccctt cgccgacctc
     5941 taccgccgcg ccctggccca tgccggccac gacccggcgg cgaccccctt cagcatcaac
     6001 tcccacggct tcatcgccga cgacagccaa acggcggccg acctgtcgtt cccgccgttc
     6061 aagctggtga tggacaagat cggccgcgag cgcggctggc cgcccatgtc gcgggcgcaa
     6121 ttcgacgagt cgcgcatgct gcgcggggcc aacttcatcg gcagccccga ggagatcgtc
     6181 gagaagatcc tcttccagca cgacatcttc cgccaccagc ggctgatgct gcaattcagc
     6241 gtcggcacca tggcccacgc cgacatcatg cgctcaatcg agctatacgg cacgcgcgtg
     6301 gccccggcgg cgcgggaaga gatcgggcgg cgaatggcgc agcaatctcg ttgacagttc
     6361 cagccgacag atataccata tcagcaagaa agccgcatga gcgagtggcc agggaggatt
     6421 cacgctcgct gcggtagttg ttccataatt gatggttgcc ttcgtgccgc attgaaaaca
     6481 taagcatctc gcagcgcctg atccaaaaga aaggcccggc acaaggacaa ccccggtcgg
     6541 tattgcctgc cgacatccgg cgacgggagg aacccgtcga ggggcgttgt tatgttcccg
     6601 ttcaaccgat tcggtcgcgt ctcgttggtc tttctgtccg ttttgctgtt cgccggagcg
     6661 tgtcgccagt tttccggcgg ccccaacacc ggcaacggcg atccggcagc cgtattcgcg
     6721 ccggacaaca acagcggcgt tgatgacacc gttgatggcg ccgccaacaa ttcagccggg
     6781 gcctatccgt ccaaagtctc cgttctgccc ggcgagagcc tggactttca tatctccaac
     6841 ggctacagcg gcaaatatac cctatcgatc tatcgcgaag gggcgacccg caccctcatg
     6901 ggcactgtgc ccaacgtcag caccggtaac tattcctgtg ccggcggcta tgacaacggg
     6961 tgcagctggc cggtgggagc caccttcaag atacccaata gctggccgag cggcgtctac
     7021 accgtcgaca tcccccggaa cagcggcaac ccgatgagga cactgttcta tgttcgcccg
     7081 gcgcagccca aggcgcgcat cctcttcctg tcctcggtca acaccattca ggcctacaac
     7141 gactacggcg gcggctcgct ctatggtttc gacaacacgg tcaaatccga gcgggtcagc
     7201 ttcgaccgcc cctacgccaa cggcgtcggc ctctatgacc gctgggagag ctatttcgtc
     7261 acctgggccg agcaggccgg ttacgggatg gattacgccg cgacctacga tctggatttc
     7321 gtgccgcgcc tgctcgacgg ctacgacctg gtcatcatcg ccggccacag cgaatactgg
     7381 acctgggcca tgcgccaacg actgagggcc tatatcgacg gcggcggccg cttcatgaac
     7441 ctgagcggca acaccatgtg gtggcaggtg cgcttcgagg acaacggccg cacgatggtc
     7501 ggctataaat cctggcgcaa ggacccggaa aagagcgccg aactctccac cgacgtgaat
     7561 tgggatcggc cgatcttcga cagctcgttc accatcaccg gcctccattg gccctacggc
     7621 ggctatcccg gcggcaccgg cgacggctac tatgtgatca aggccaatca ttggatctat
     7681 caggggacgg ggttgacgga aaatacgctg ttcggcaagg ggccgacggt cgatacctcg
     7741 atccacgaca aggagtcgga cgggctggcg tttaactgcg ccgccgacgg ttcgaccctc
     7801 ctcggccctg tcgcgggcac cggcacgccc gccaacttca ccatcctggg gctgacgcga
     7861 gtccacagca agcagcgcga cctcgacggt gtggccatga tgggcctcta tacgacgccg
     7921 gcgggcggcg cggtcttctc ggcgggcaca accggctggg tgctggggct ggggcagccg
     7981 gcggtagatc gtatcacccg caacgtcatg gatcgcttcc tggccggcag cttcccgcag
     8041 gagccggccg cgcccgacag cggccatctc ttcgccgacc gcttcaactg caccgatctc
     8101 ggccgcggcc ggttcctggc gacgctcaac gacgccgccc ggctcaacta cgtcatcacc
     8161 gagaaggcca ccaccgcccg actgaccgcc gcctgcgggg cggacggcgc gggcttgcag
     8221 ctccccgccg ggtcgaccgg ggtgcgcttt gtggccgggc tggggccaaa ctggcgatcc
     8281 aatttcgcaa tcgaaacgca ggtcgatctg aacctggcgg ggttcagtat cgacaaggga
     8341 gcgaccctca ccctgatgac ccattacgcc gacaaccggg tcgacgcgcc ggtgacggtg
     8401 gcggcgctgc agctcggcaa cgtggacaat gtcctcagcg cgcgctatca gccggcgggt
     8461 ggcccggaga tgccctgggt gcgcgtgccg gcgaacggct tcttccgtgt gaacacaacc
     8521 tgggacggcc agcgcgacaa ggtcggcctg accatcgacg gcagcggcta tgagcggcag
     8581 gtggaattgg gcaattcccc cctgcccaac cgggccgatc acggcattct gcagaagcaa
     8641 ggcgccagcg ggggcacgat ttgcatggat aatctgatct acgacggtcg gccgtttgga
     8701 gtggaaccaa cgcccacccc cacgccgaca gccaccccca cgccgacagc tacccccacg
     8761 cggacaccca cgccgacgcc gacggccacg cccacaccga cagccacgcc cacgccgtcg
     8821 ggcgacggca tccacatcgg cgatctggac ggcggcagcg agaacatgcc caacagccgt
     8881 tggcgcgcca cggtcaccat caccgtccac gatgccaatc atgtgcccgt ggccagggca
     8941 agggtgcggg gcaagtggag cggcggagcg agcggaagtg gctcatgcat gaccaatgcc
     9001 gggggcacgt gcagcctgga caagatcgtg ctgtcgcaga acagcagcgc gaccttcacc
     9061 gtgaccaagc tctccaaaac aggagccacc taccgcagca cggccagcca tgacgtggac
     9121 ggcggaacga acgggacggt gattgtggtc aatcggccgt agtgcaatca gaaattacga
     9181 attacgaatt acgaatcagg aatttaggaa atacgaacga cgaagcacga agcacgcgct
     9241 cgtcgttcgt acttcgtcat tcggctattg gtcgatcacg acgggcagga acagggcaaa
     9301 gtcgcctatc ggcggcagcg ttgccgtcgg cgatggcgtc gcttctattg tcggcgatgg
     9361 cgtcgcttct gatgttggcg agggcgtcgc ctctatcgtc ggcgatggcg tcgcttcggt
     9421 tgtcggcgag ggcatcggcg agggggtcac cgtcgcttcg gttgtcggca gtggcgtggg
     9481 cgacgggcca tcggtggccg tgggcagcgg cgtcggtgtc tttgtcggcg tcggcgatgg
     9541 agtcgcggtc ttcgttggcg tcgcggtcgg gccgccgtca cccagcgcca gcagcgcctg
     9601 atacgcatcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggcgtcgcg
     9721 gtcgggccgc cgtcacccag cgccagcagc gcctgatacg catccagcat gccgtcgccg
     9781 caacttgccg tcgtacagtc gctaatgtcg gggaagtcgc gcgcggaatc ctgaaggagc
     9841 tcgctcacct ggacgggcgt caggccgggc ttctccccca gcagcagcgc cgccaccccc
     9901 gccacgtggg gcgcggccat gctcgtgccc tgatagaagg ccagactgtc ggccgccggc
     9961 cccctcgtgc cgctgttgag ggtggacaat atgccgtcct cctcgtacca cgtatcccca
    10021 ccgggcgcgc tgatctcgac cgtggagccg tagttggaat accaggccaa atagccgtag
    10081 cggtcggtgg cggcaacggt gatgacgcct ttgcaactgg ccggggtata gaagtcggcg
    10141 attttggagc tgttgccggc ggccacgacc accaccgcgc cggcggccgt gacgtcgtta
    10201 aaagccgatt gccacgacgc cgggcaagtg cccgaaccgc cgaggctcag gttcagcacg
    10261 cgggcggggg tggcattggc gggcacgcca gggacggcca gcccggccgc ccaacgcacc
    10321 gcgtcgatga tgtccgacgg ataaccgccg cagcgcccca acacgcgcac gggcaatatt
    10381 ttggcctgcc aattgacccc ggccacatcg tcaccgttgt tggaggccgc gccgatggtc
    10441 ccggccacgt gggtgccatg ccaggagctg ggggagaaag ggtccccgtc gcattgcccg
    10501 gcctcgttcc agtcgccggg gtcggacggg tcggggtcgc ggccgtcgcc gtcgttggcg
    10561 gtggccgggt tggtgataaa gtcatagccg ggcacggtgc ggccggccag atcggcgtgg
    10621 ccggtgatgc cggtgtcgag cacggcgacc acgatgtcgg ccgaaccggt ggtgatggcc
    10681 caggccggct ccagattggc cccttcctcg tcgcccggcg tatagcgata atgccattgg
    10741 tcggcaaagc cggggtcgtt gggcgattga tcggccgcgc gcggggtgcc ggtgagaggc
    10801 gacaccaccc gaatgcccgt atgacgcatg atgatgtcgg gttcggcgta gtcgatctcc
    10861 ggcagcgcag ccaggtcgcg ggcgatggcg gccacctgat ccacggcgag cggcttgggc
    10921 agccgataga cgaacgcatc gcccgacatc ggccgcacga aggtcagcgt cgtgccggcg
    10981 gcggcgttca tacgatcgag cagttgttgc cgcgcggccg cgtcgggcgc gcgcccggca
    11041 atcggagcga accggatcat gagctggtcg gcaggccgat cggccggctg cccggcgcgg
    11101 gcaggggacg gctcgatggc gcgggcggca gtgcggccgc cgggcagcag ccacagcgca
    11161 accactaacg acgccagaaa agcgatcgcc aaaagtccgt gtcgcatggt gggctttcga
    11221 ttgtgatcca tgtttgctcc tgcgtccgca ctttgtgaaa ctgcaagcct gcaaaagtat
    11281 agtcaaatgg gccgggcacg gcaattgggc gcatcagcaa ccgccaagcg ggcattgccg
    11341 ttgcccaagg aagggagtat atttcggtga aacggcaaat gccggttttg tcgttggtgg
    11401 tgggccgcgc aacaggagac aaacgttacc tctattattc gcgcgggaga cgggtgtctc
    11461 gtgactgccg cttgccggcg ccggaaggaa caagaccatg ttgggtgcag ctttattgat
    11521 cgcggccgcg ctgctggccg gaattggttt gctaatctat ctgccggcgc gagcggcaac
    11581 gcccgaaaag cccaatatcg tgatcgtcat gtccgacgac caggacatgg actctctgcc
    11641 cgtcatgcgc aagctgatgg ccaaccccga aggctcatgg gtcaacttca cccatgcctt
    11701 cagcaacacc agcatctgca cccctgcccg cgccactctg ctgaccggcc agtattccca
    11761 caccaacggc atcctgggca acaattgggg cgcggatttc aacaacgaca acacgttggc
    11821 cgtttggctc gatgatgccg gttacaagac tgcgctcatc ggcaagttcc tccattatca
    11881 gcctggcgaa aatggcgacg agcgagggca gtatgatatg cccggctggg acctgtactc
    11941 gatctcctac ctgactgtgg atgaacacac gcaagaggca gtcgatttca tcagtgatgc
    12001 caaatcaccc ttcttcctgt ggctggccta tcgcgcaccg cacaaaacag ccgatccgcc
    12061 gcctcgttat gagggcattg acgtctacat ccctcccgac cggcccaatt tcaacgagaa
    12121 ggatatgtcc ggcaagccgg agtatatgcg caaacaaccg ctgttgacgc ccgaggaaat
    12181 cactggactg cgcaaggagc gcgccaactc gcagcgcgaa ttgctggccg tcgacgacgg
    12241 catcgagtcg gtcgtgaacg cgctcaaggc caagggcgag ctggataaca cgatgatcat
    12301 cttcgtgggg gacaacggct tctcctgggg ctcccaccgc ctgtatgaga agctctgccc
    12361 ctatgaggag tgcagcatgg tgccgctctt cattcgctat ccggggacag gcgagaaccg
    12421 catcgaggag cattttgtgt cgatggtcga tttggcggcg accatcgccg actacgccgg
    12481 ggtgaagccg aaattgccgc aggacggccg cagcttcctg cccctgctga atggaaccgc
    12541 tccctggtgg gacgacaccg tgctgctgga aaaagtcgcc ggccgcaacg cccattacgc
    12601 ggcgcggtcg ccgggttgga cctatgtgga atacatcacc ggcgagaagg agctatacga
    12661 cctgacggct gatccgtatc agctgaagaa tttggccgga aagccgggct atgccgctca
    12721 gcaggcggtg atggcccaga agctctatta tctattgaac ccgccggctc ccacacccac
    12781 agcgacccca acggtgtcgc cgaccccaac ggtgtcgccg acgccaacag ggacgccgac
    12841 cgcaaccgga acaccgtcgc cgacatcgac accgtcgcta acgccgactg atgggccatc
    12901 gcccacaccg acggccacgg agacgccatc accaacggct accgacggtc catcgcccac
    12961 accgacagcg acggagacag caacaccgac ggagacgcca tcgccaacag ctaccgccgg
    13021 cccgtcgccc acgccatcgc cgacagccac ggccgcccca cccgaccggg caatctattt
    13081 gccgatcatc accggcggcg cggccgttcc tgtgccatag gtgtggcgca agccgccggc
    13141 ttgcgttcac ttttgccaat gaatacgggt acagatacga gggcctggcg accgggaaat
    13201 aggtgtggcg caagccggcg gcttgcgttc acttttgcca atgaatacgg gtacagatgc
    13261 agaggtccgg cgaccgggaa aaacgtagtt ggtgcgtggg gcgggtttgt tgcatgttaa
    13321 caaaataatc acgtatcaat caagcgcggg atgaatcccg cggctgttat gccaaaccga
    13381 ctgaagtcgg ttgccgcctc tgccggcaac cggtttcaac cggttttgtc tatcagcagc
    13441 ggatttcaat ccgccggagc gtatacgtga ttaatttgta aatattcaaa aaacccgccc
    13501 ctgcgactat cccgcaatag tccgttaatt tcccttatgt tgacccggcg atattaatga
    13561 tatacttata ttgtcataat tcgtc
//
LOCUS       DAOMQG010000051        13582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1945_length_13582_cov_11.982553, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000051 DAOMQG010000000
VERSION     DAOMQG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13582
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_13582_cov_11.982553"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PLC06_04745"
     CDS             <1..1213
                     /locus_tag="PLC06_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRO89785.1"
                     /translation="ILLIALATRNLLRRQVERVEARLRQVGIVAEQFPWSVDLLVALG
                     ASIFPLVVWLLCTIAAGALTSAERPADVLAWLAPIFGLVGVYRFVGTIMTLNLSAERA
                     RTWRYQILRPLIAVLLFLHITGMLDNLMGLRLNPGQGVLVTLQSALMAVAALIFFFWL
                     SRIIRRFLAETALPRAGVEPAVNQVISTFSGYALVIAGFLVALSVMGINITTLTVIAG
                     GVSVGIGFGLQELINNFVSGFILLTERSLAPGDVIEVDGNMGRVQQIGLRTMQITTPD
                     DVELIIPNGYLLSSTVTSYTHKSPEMRVHIKLPAAAEVHPRTVQETLLEAARTHSEVL
                     AEPGPTALVVDFEDQVIRYDLQFWIQNPIRAPFITSDVRVRIWELFAERGIEMDVPQD
                     VLLVKSSEQGD"
     gene            complement(1210..2184)
                     /locus_tag="PLC06_04750"
     CDS             complement(1210..2184)
                     /locus_tag="PLC06_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRO89786.1"
                     /translation="MEYRRLGRTGLKVSTICLGTMQFGWSTDEPTAHVVMSRAVEMGI
                     NFIDTADVYSRWVDGNPGGVAETHIGNWLASGAVSRDRLVIATKVRGTMGDGPNDKGL
                     SRSHIMNAVEASLRRLKTDYIDLYQTHSFDPETPLDETLRALDDLVRDGKVRYIGCSN
                     YPAWQLAKSLWISDLRNLARYDSVQPHYSYVHRAEFERELQPLCLDQGVGVIPYSPLA
                     AGFLTGKYRRDAPLPDSKRVESIQRRFMTERGFAAVDKLEAVGQAHDATIAQTAIAWV
                     LANPAVTSAIIGATSIAQLEDTTKGAEVKLSAEDKAALDEVTAWKDEG"
     gene            complement(2267..3766)
                     /locus_tag="PLC06_04755"
     CDS             complement(2267..3766)
                     /locus_tag="PLC06_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRO89787.1"
                     /translation="MLKALRRILLIALLVVGAAYLLYQGFLFWRAMDKLPPTAIVAGV
                     PVGGLTMDAARDAINEKFNAPLWVYNGEQRAAELLPRDVGFSIDTEGMLAEAKAEWEQ
                     QEMWLRYVEFVLGISPKPIVVYIRARHDDAALRGQLDLISDFIDRPAQGPQLLSDTSE
                     IQPGRPGTITDRETTMYGLRSALYDPENRRVDMTLIEEPAPEWDMRVLQEAIENQLAS
                     FDGFASVFILDLQTGEEVRINSDVAVSALSIMKIAIFVEAYRTLDNPPTAFEQELFFS
                     TATASSNHSANLLLHLIAGEDNTYEGAAVLTDEMHEMGMVNSFMAIPYDAVAVPSRPS
                     TYVTPANQNPTIDTRPDPSMQTTAEDIGSLLAMLYYCAKGEGGLLAVYPGEITQEECQ
                     AIVDLMVQNVEGNLIRFGVPDGVRVSHKHGWSFNEHGDAGIVYSPGGDFVIYTLLAQP
                     ESDWLSSEYSFPFLWEISRAAYNYFNPDKPFEGHSAVELERRESIRAGQ"
     gene            complement(3777..5270)
                     /locus_tag="PLC06_04760"
     CDS             complement(3777..5270)
                     /locus_tag="PLC06_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRO89788.1"
                     /translation="MNRNASPGLGQWITVSIMVVVSLFLLYKLYQYAGTRTEFPTGLT
                     IGGVDVGQMNPDEARAKLTATFIDAPVTLYHGEDRFDLVPSQAEFTLDFDTMLTAADK
                     ERTGQDFWSGFWGFLWGRPIEVNAVPLVATHNREALRNYLEEIKSLVDLPAQPPQPVP
                     GSMSFAYGTPGTTTNIEASFADIEAALYRPNTREARLIIEPKNPERPNINLLTRLLVN
                     RLQVFEQDTGGMAGVFIMDLATGDEVNINSDVPVTAIDLMKLPIVLETYNTLSQLPTL
                     SQRQLISNTLVINPDNTSANELLTFIGGEEGPYVGAERVTAAMQRLGLVNTFIMAPYD
                     AALPAGKRTPETPGNSVEGLRTNPGATMQTTAEDIGTLLSMIYYCATGQGGALVAAFP
                     ANDWQRECLEMLDYMERNRIGALLEEGIPGETTIAHRHGWVGDTHVDAGIVFSPSGDY
                     VIVEIMYRPEWLEWELSAPLIADVSRAAYNYFNFDAPYLGQTSAVNN"
     gene            complement(5283..5795)
                     /locus_tag="PLC06_04765"
     CDS             complement(5283..5795)
                     /locus_tag="PLC06_04765"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRO89789.1"
                     /translation="MNIQLTDTWKEGLARLIRRRPFKGLLAWGVRLIVPRQRVGVALV
                     TFNENEEVLLLRHVFHTASSWGLPGGWLNRNESPQDGLVRELGEETGRELIIGPPIQA
                     AHEGPPSHIVMAYLGWLQPGPMRLNSEIIEARWFDLDRLPQPLWSFTESAISAGLSLK
                     RLLADEPVAQ"
     gene            6033..6434
                     /locus_tag="PLC06_04770"
     CDS             6033..6434
                     /locus_tag="PLC06_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRO89790.1"
                     /translation="MQQTRLMRSETDKMIAGVCGGVAAYLGVDPVLVRLAFVILSLAS
                     GVGLAMYIILWIVMPTESRVPPEIRIMDEGAASDPSAYKTKLGQPATIGIILILLGVF
                     LTLGQWGWTPKFIWPLILIGAGLFYLIRRTR"
     gene            complement(6473..7984)
                     /locus_tag="PLC06_04775"
     CDS             complement(6473..7984)
                     /locus_tag="PLC06_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89791.1"
                     /translation="MSIQNRLLLVYALIFSIVYILSSLVIYILPRNQLLAQIDGDLYS
                     LAAELMTGNIDVGADGIIRMPLPSDLATLQTASTFLIIVNREGKIVVQSSNLAGFDQL
                     LDPDSLGTQEVYRLVPHEDTLLRVLTVPVFDNRQQPIGHIQVARLLDNFEIFNRVLSR
                     ALLISLGAAIASLIVLAMLTSSLFKPLEDIASVARQITRADDLSRRVPYSSRTDEIGD
                     LARAFNQTLERLDRLFRSQQRFLADVSHELRTPLTSVRGNLDLMRRFGRYDEESMEVI
                     QDEMERMSRLLGDLLLLARADTGGLPLRHEPVDLDSILIEVYRQVSRIEKPVTLELTA
                     VDQARIMGDEDRLKQLLLNLVDNGIKYTQPGGTVRLSLSQENGWADLVVSDTGIGIPP
                     EDLPHIFDRFYRVDKARSRAQGGSGLGLAIAKWIVQAHGGTMKVESTVGEGTTFRITL
                     PLYQSLGTGQVADDVEVKTRPGIRRAALPGTARRGRQANEKEGNQKEKRDETA"
     gene            complement(7992..8669)
                     /locus_tag="PLC06_04780"
     CDS             complement(7992..8669)
                     /locus_tag="PLC06_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89792.1"
                     /translation="MSSTILVVEDEERIRQFLQRGLGFEGYRVDAAPDGPTALELARD
                     NPPDLVLLDLMLPGMDGLEVCRRLRAVSDVPILMLTAKEAIDDRVAGLDAGADDYLVK
                     PFAFDELMARVRALLRRAQPAQPQVFRFADLELDTGTRQGRRGGETFDLTAKEYELLE
                     LFMRHPRQVLTRDVIFDHVWNYDFGGESNIIEVYVRYLRQKTEQGDMARLIHTVRGIG
                     YVLREES"
     gene            8848..9840
                     /locus_tag="PLC06_04785"
     CDS             8848..9840
                     /locus_tag="PLC06_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRO89793.1"
                     /translation="MEELNQLSIALATLMQEAYPRLLGFMTFISALGTEEFFLLALPV
                     VYWCVNKQLGRQLGYFFLASALINNVLKNLFRQPRPFWLMPSIQRSDIEGFGLPSGHV
                     QNVTTVILILAAHVRRGWFWLSGLFFIFLMGLSRLYLGVHSLQDIILGFVIGLLLFGI
                     FIAWQQLFYERYSNQILGRRLLIMILVPIILAAVYIGLFFIVGPADTSVSWAEYIPAA
                     ELNGYEDIVSSLAALFGFGIGILLESSRIRFMVDGPLWLRAIRYVLGMTVALGIWAGL
                     RAVFPAEPLWLAMPFRFLRYLLLLFWVTYFAPWVFVKLRLASANPESEVRVTFS"
     gene            complement(9907..10836)
                     /locus_tag="PLC06_04790"
     CDS             complement(9907..10836)
                     /locus_tag="PLC06_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO89794.1"
                     /translation="MRFLVTGGAGFLGAALANRLVADDHRVHVLDDLSNGDAARLDPR
                     VEFTKGDVDNVPLLWSLLQSADCVYHLAARVSVPQSVLHPREYARVNVGGTVSLMEAM
                     RDTGVGRVVFTSSGAVYGNQPRQPVREDVTPFPDSPYAVSKWSAEQYIHTIGALWGME
                     TVALRIFNAYGPGQSLPVSHAPVVPRFLKQALSGGSIVVFGDGRQTRDYVYVDDVVTA
                     LVAAATAQNVNRSIINIGSGREITILDLIAHMERVTGKRANVIHNAEKSGGVPHLAAE
                     ISKARELLGFRPEIDLETGLRLTIERDPRFSSR"
     gene            complement(10866..11624)
                     /locus_tag="PLC06_04795"
     CDS             complement(10866..11624)
                     /locus_tag="PLC06_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89795.1"
                     /translation="MSTETGHSSESTIPTAREAAQLLTHLVEARPGKHVKVEAGELPP
                     EIRLLREWQSTRLAWTHHDLLEHPRFRPATRFFLSDVYAPRDFSQRNYDIQKAYDAMR
                     KAVPERVVHTLAETIELHQFTDELDAKLADALFDQLSVEHITPQSYAEAYRICDNYDE
                     RVRQIDMITKIGRSVDRIVRLPFIGLTLRLAHTPAHLAGWHELQGFLERGYAAFKHMK
                     GAEEFLNTIETRETGILDRIYAGDPDPFDLVEQG"
     gene            11801..12388
                     /locus_tag="PLC06_04800"
     CDS             11801..12388
                     /locus_tag="PLC06_04800"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO89796.1"
                     /translation="MTGISKEVLSEEQFDQLLRFFQVFSNESRLKIIGHLANGEKNVG
                     ELAGLLRVKEPTVSHHLAEMKAIGLVNVRAEGTMRIYRLNSRALEAMSKDIFAHSNLA
                     ALVKTSELSEDEKILRTWVKDGRIIDLPAQEKKKQVIIHWLADRIPHDRRWTEREFSE
                     WLTQFNEDYATLRRYLVDSGYMTREKGIYWRTLKA"
     gene            12496..13122
                     /locus_tag="PLC06_04805"
     CDS             12496..13122
                     /locus_tag="PLC06_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO89797.1"
                     /translation="MIEAPALPKPASEIMPLVVKFRPLYPMTPDDFQEFCALNPDLVV
                     ELTAEGEIVFMSPSGGTTGARNAKVIIALGQWANEDGRGVVFDSSTGFVLANKAVRAP
                     DAAWVRRSSLAKLTPPQKERFLPVTPDLVVEIASPSDRESELQPKMEEYIANGVQLGW
                     LILPETRSVIVYRPGKKGELLSDIDSISSDPVLPGFTLDLRPIWMPDF"
     gene            13260..>13582
                     /locus_tag="PLC06_04810"
     CDS             13260..>13582
                     /locus_tag="PLC06_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HRO89798.1"
                     /translation="MHDVLIRNSLIYDGSGKPPYSADLAITGDTITEIGRIDGRAQLT
                     IDADGLAVSPGFINMMCWANESLIHDGRSQSDIRQGVTLEILGEGWSMGPLNDELKAY
                     TKELQG"
BASE COUNT         2831 a         4106 c         3821 g         2824 t
ORIGIN      
        1 catcctgctc atcgccctgg cgacgcgcaa cctgctgcgg cggcaggtgg agcgggtgga
       61 ggcgcggctg cggcaagtcg gcatcgtcgc cgagcaattc ccatggtcgg tcgatctgct
      121 ggtagcgttg ggggcgtcga ttttcccgct cgtcgtctgg ctgctgtgta ccatcgccgc
      181 gggagccttg accagtgccg agcgcccggc tgacgtcctg gcctggctgg ctcccatctt
      241 cggcctcgtc ggcgtctacc gcttcgtcgg cacgatcatg acgctgaacc tgtcggccga
      301 gcgcgcgcgt acctggcgct atcagatatt gcggccgctc atcgccgtgc tcctcttcct
      361 ccacatcacc ggcatgctcg acaacctgat gggccttcgc ctcaatcccg gccaaggcgt
      421 gctggtgacg ctgcaatccg ccctaatggc cgtggccgcg ctcatcttct tcttctggct
      481 cagccgcatc atccgccgct tcctggccga gacggcgctg ccgcgggcgg gggtcgagcc
      541 ggcggtcaat caggtcatca gcaccttcag cggctacgcg ctggtcattg ccgggttcct
      601 cgtggccctc tcggtcatgg gcatcaacat caccacgctg acggtcatcg ccggtggcgt
      661 ctccgtcggc atcggcttcg gcctgcagga gctaatcaac aacttcgtca gcggttttat
      721 cctgctgacc gaacgcagcc tcgccccggg agacgtcatc gaagttgacg gcaacatggg
      781 cagggtgcag cagatcggct tacgcacgat gcagatcacc acccccgacg atgtggaatt
      841 gatcatcccc aacggctact tgctgagcag cactgtaacg agctacaccc acaaatcgcc
      901 ggagatgcgc gtccacatca agttgcctgc cgcggccgaa gtccatccac gcacggtgca
      961 ggagacgctg ctggaagcgg cgcggacaca ctccgaagtg ctggccgaac cggggccgac
     1021 tgcattggtg gtcgattttg aggatcaagt catccgctat gacctgcagt tctggatcca
     1081 aaaccccatc cgcgcacctt ttatcaccag cgacgtgcgc gtgcgcatct gggagctgtt
     1141 cgccgagcgc gggatcgaga tggacgtgcc gcaggatgtc ttgctggtga agtcaagcga
     1201 acagggcgat taaccctcat ccttccacgc tgtcacttcg tccagcgccg ccttgtcctc
     1261 ggccgacagt ttcacctccg cgcccttcgt cgtgtcctcc agctgggcga tcgacgtcgc
     1321 gccgataatt gccgacgtga ccgccggatt ggccagcacc caggcgatag ccgtctgggc
     1381 gatggtggcg tcgtgggcct ggccgaccgc ttccagtttg tccacggccg cgaagccgcg
     1441 ctcagtcatg aagcgccgct ggatgctctc gacccgtttc gagtccggca agggcgcatc
     1501 gcggcggtat ttgccggtca ggaagccggc ggctaacggg ctgtagggaa tcacgccaac
     1561 cccctgatcg aggcacaacg gctgcagctc gcgctcgaat tcggcgcgat ggacatagct
     1621 atagtggggt tgaacgctgt cgtagcgcgc cagattgcgc aggtcactga tccacaggga
     1681 tttggccagc tgccaagcag gatagttgga gcagcctata tagcgcacct tgccatcgcg
     1741 caccagatcg tccaatgccc gcaatgtctc atcgagcggc gtctccggat cgaaggaatg
     1801 ggtctgatac agatcgatat aatcagtctt caggcgacgc aggctggcct cgacggcgtt
     1861 catgatatga ctgcgcgaga ggcctttatc atttggcccg tcgcccattg tccccctgac
     1921 cttggtagcg atgacgagcc ggtcgcgcga gacggcgccg ctcgccagcc agttaccgat
     1981 gtgggtctcg gccacgccgc cggggttgcc atccacccag cgactataaa catcggcggt
     2041 gtcaatgaag ttgatgccca tctccacagc acggctcatg acgacatgag cagtcggttc
     2101 gtcggtcgac cagccaaact gcatcgtgcc cagacaaatc gtgctgacct tcaggccggt
     2161 gcggccgagg cgtcgatatt ccatttcact ctccttatat tcaatgatgg ggtgagatgc
     2221 catcccttca acgggttggc caagattaca ggcgcacctg cgccgattat tgtccggcgc
     2281 gaatcgattc ccgccgctcc aactcgaccg cggaatggcc ttcaaacggc ttgtcgggat
     2341 tgaagtagtt ataggctgcg cgcgagatct cccacaggaa cgggaagctg tattccgatg
     2401 atagccaatc actctccggc tgcgccaaca atgtgtagat gacgaaatca ccgccgggag
     2461 agtagacgat ccccgcgtcg ccgtgttcgt tgaagctcca gccgtgcttg tgggagacgc
     2521 gcacgccatc aggcacgcca aaacgaatca ggttgccctc aacgttctga accatcaaat
     2581 caacgatggc ctgacattct tcctgggtga tctcgcccgg atagacagcc agcaagccgc
     2641 cttcgccctt ggcgcaatag tagagcatag ccagcaaact gccgatatcc tcggccgttg
     2701 tctgcatgga agggtcgggt ctggtgtcga tcgtcggatt ttggttcgcc ggggtcacat
     2761 aggtgctggg ccgtgaaggg acagcgacgg catcatacgg aatggccatg aagctgttga
     2821 ccatccccat ctcgtgcatt tcatcagtca aaactgccgc accctcatag gtattatctt
     2881 ccccggcgat caggtgcagc aacaggttcg cgctatggtt gctcgacgcc gtcgccgtgc
     2941 tgaagaatag ctcctgctcg aatgccgtag ggggattatc cagcgtgcga taagcctcga
     3001 caaagatggc aattttcatg atgctgaggg ccgacacggc cacatcggag ttaatccgca
     3061 cttcctcgcc ggtttgcagg tcaaggatga acacgctggc aaagccatcg aacgaagcca
     3121 actgattttc gatggcttcc tgaagaacgc gcatatccca ttcgggcgca ggttcctcaa
     3181 tcagcgtcat atcaacccgc cgattctccg gatcataaag ggccgagcgc agcccgtaca
     3241 tggtcgtctc tcgatctgtg atcgttcccg gccggccggg ttgaatctcg ctggtatcgg
     3301 agagaagttg cgggccttgg gccggacggt cgatgaagtc ggagatcaag tccaactgac
     3361 ctcgcaaggc agcatcatca tggcgtgccc ggatatagac gacgatgggc ttgggcgaga
     3421 tgccgaggac aaactcgaca taacgtaacc acatctcctg ctgctcccat tccgccttcg
     3481 cctcggccag catgccttcg gtgtcgatgc tgaacccaac atcgcggggc agaagctcgg
     3541 ccgcgcgctg ctcgccatta tagacccaca atggcgcatt aaatttctca ttgatggcgt
     3601 cgcgagccgc gtccatcgtc aggccgccga ccggcacccc ggccacgatg gccgtaggcg
     3661 gcagcttgtc catcgctcgc cagaacaaaa agccctggta gagcagatag gccgcgccca
     3721 cgacgagcag ggcgatcaaa aggattcggc ggagagcctt caacatgccg gtcttctcag
     3781 ttattgaccg cactggtctg gcccagatag ggcgcgtcga agttgaagta gttataggcg
     3841 gcgcgggaga cgtcggcaat gagcggcgcg ctcaactccc attccagcca ttcgggtcga
     3901 tacatgatct cgacgatgac atagtccccg ctggggctga agacgatgcc cgcgtccacg
     3961 tgcgtgtcgc caacccagcc atgccggtga gcgatagtcg tttcgccggg gatgccctcc
     4021 tcaaggagag cgccaatccg gttgcgctcc atataatcga gcatctccag acattcacgc
     4081 tgccaatcgt tggccggaaa agccgcgacc aacgccccac cctgcccggt ggcgcaataa
     4141 taaatcatcg ataacagggt gccgatatcc tcggccgtcg tctgcatcgt ggcgccgggg
     4201 ttcgtgcgca ggccttccac tgagttgccc ggcgtctccg gtgtccgctt tccggccggc
     4261 aaggctgcat cataaggagc catgatgaac gtgttgacca gccccaaacg ctgcatggcg
     4321 gccgtcactc gctccgcccc aacgtaaggc ccctcctcgc cgccgatgaa agttaaaagc
     4381 tcgttggcgc tggtattatc gggattgata accagcgtat tggagatcag ttggcgctgc
     4441 gaaagcgtcg gcaattggct aagcgtattg tacgtctcaa gcacaatcgg gagcttcatc
     4501 aaatcaatgg ccgtgacagg cacatcgcta ttgatgttga cttcgtcccc cgtagccaga
     4561 tccatgataa acacgccggc cataccgcct gtatcctgct cgaacacctg caagcgattg
     4621 acgagcaagc gcgtcagcaa attaatattc ggccgctcgg ggttcttggg ttcaatgatc
     4681 agccgcgctt cacgcgtgtt cgggcgatag agagccgcct caatatcggc aaagctggcc
     4741 tcaatattgg tcgtggtgcc cggcgtgcca taggcgaagc tcatgctgcc ggggacgggt
     4801 tggggtggct gggcgggcaa gtccaccagg ctcttgatct cttccagata gttgcgcagc
     4861 gcctcgcgat tatgagtcgc gacgagcggc acagcgttga cctcaatcgg gcgaccccat
     4921 aaaaagcccc agaaccccga ccagaaatcc tgcccggtac gttccttgtc ggccgccgta
     4981 agcatcgtat cgaaatccag ggtgaactcc gcctgggacg gcaccagatc gaacctgtcc
     5041 tcgccatgat acaacgtcac cggcgcgtcg ataaatgtag ccgtcagctt ggcgcgcgct
     5101 tcgtccgggt tcatctggcc cacatcgacc ccgccgatcg tcagtccggt gggaaactcg
     5161 gtacgcgtcc cggcatactg atagagcttg tacagcagga acagcgaaac caccaccatg
     5221 atggagacgg tgatccattg accaagcccg ggggatgcgt tgcggttcat acagtattcg
     5281 tgttattgcg cgacaggctc gtcagccagc aatcgtttaa gcgacaggcc cgccgaaatg
     5341 gcggattccg tgaatgacca gagcggttgc ggcagccggt caagatcgaa ccagcgagcc
     5401 tcgataattt cagaattcaa acgcatgggg ccgggttgca accaaccgag ataagccatc
     5461 acgatatggg atggcgggcc ttcgtgggct gcctgaatgg gcgggccgat gatcagttct
     5521 cgacccgtct cctcaccaag ctcgcgaaca agcccgtcct ggggagattc atttcggttc
     5581 aaccacccgc ccggaagccc ccaggagctg gctgtatgaa acacatggcg caacaacaaa
     5641 acttcctcgt tctcgttaaa agtgaccaac gcaacaccga ccctttggcg cgggacaatc
     5701 agtcgtactc cccaggccag caaccccttg aacggccggc gacggataag ccgcgcaagc
     5761 ccttctttcc aggtatcggt caattgaata ttcacaatac ggtcactatg cagttaatac
     5821 tgggcctgtc gcatcaacgt acaggcagca atattatcct ttcctataat cccacagtat
     5881 aacaaaaagg cccttcgatt ccataggcat tgcttcccgg tcatcctctt ctcatatgtg
     5941 tatattcgac tattcgcctc gaaaatgggc cgcaactttg gtgctgcgtc agcgtataga
     6001 agataaatat tgagatcgtg gaggtagtcg gcatgcagca aacaagactg atgcgcagcg
     6061 aaacagacaa gatgatcgcc ggggtgtgcg gcggcgtggc cgcttacctg ggggttgatc
     6121 cggttctggt gcgattggca ttcgtcatcc tgtctctggc cagtggtgtc ggtctggcga
     6181 tgtacatcat tctgtggatc gtgatgccca ccgaatcgcg cgtccccccc gagatccgaa
     6241 tcatggatga aggagcggcg agtgatccat cggcctacaa aacaaaattg gggcagccgg
     6301 ccacaatcgg tattatcctg atcttgctgg gcgtgtttct gacgctaggt caatggggct
     6361 ggacaccgaa attcatctgg ccgctcatcc tcattggcgc cggattattt tatctgatcc
     6421 gtcgcactcg ctgacccggc atcgagcaat tgcaaacggg atgagatagc ctctaggcag
     6481 tctcgtcccg tttttctttc tgattccctt ctttctcgtt cgcttgccgg ccgcggcgcg
     6541 ccgtgcccgg caacgcagcg cgccggattc ccggacgggt cttaacctct acatcatcag
     6601 ccacctgccc cgtgccaagc gactgataga gcggcagagt gatccgaaac gtcgtccctt
     6661 cgccaaccgt gctctccact ttcatcgttc ccccatgtgc ctggacgatc catttggcaa
     6721 tcgccaggcc caggcccgaa ccgccttgcg cgcggctccg ggctttatcg acccggtaga
     6781 atcgatcaaa gatatggggc aaatcttccg gcgggatgcc gatgccggta tcactgacga
     6841 ccagatcagc ccatccattt tcctgcgaca ggctcagccg aacggtcccg ccgggctgag
     6901 tatatttgat gccgttgtcc accagattga gcaacaactg cttcagccga tcttcatcgc
     6961 ccatgatccg cgcctgatcg acggccgtca actccagcgt caccggtttc tcgatgcggc
     7021 ttacctggcg gtatacctca atcaggatgc tgtccagatc aaccggctca tggcgcaacg
     7081 gcaacccgcc cgtgtcggcc cgcgccaata acagcaaatc acccagcaga cggctcatgc
     7141 gctccatctc atcctggatg acttccatgg actcctcgtc ataacggccg aagcgccgca
     7201 tcaggtccag attaccgcgc acgctggtga gtggtgtcct taattcatgc gaaacgtccg
     7261 ccaggaaacg ctgctggctg cggaaaaggc gatcaagccg ttccagggtc tggttgaagg
     7321 cccgtgccag atcgccgatc tcgtccgttc ggctgctata gggcacacga cgactcaaat
     7381 cgtcggcgcg ggtgatctgg cgcgccactg aggcgatgtc ctccagcggc ttgaaaaggc
     7441 tggacgtcag catggccagg acgatcagcg aggcaatggc cgcgcccaga ctaatcaaca
     7501 gcgcccggct gagaacacga ttgaatatct cgaaattatc aagcaggcgc gccacctgaa
     7561 tatggccgat gggttgttgc cgattgtcaa acaccggtac ggtcaggact cttaaaagcg
     7621 tgtcctcatg gggcacaagc cggtaaactt cctgtgtgcc caaactatcg ggatccaaca
     7681 actgatcgaa tccggccaaa ttgctggatt ggacgacgat cttgccctcg cgattgacga
     7741 tgatgaggaa tgtcgacgcc gtctgcaacg tcgccaaatc ggaagggagc ggcatgcgaa
     7801 tgatgccgtc cgctccgacg tcgatgttgc cggtcatcag ttcggcggcc agcgaataga
     7861 ggtcgccatc gatctgcgcc aatagttgat tgcgcggcag gatatagatg accagcgacg
     7921 acaggatgta gacgatgctg aagattagag cataaaccag cagcaatcga ttttggattg
     7981 acaactaggg gctatgactc ctcgcgaaga acgtaaccga ttccccgcac ggtgtgaatc
     8041 agtcgggcca tatcgccctg ttcggttttc tgccgcaaat agcggacgta aacctcaata
     8101 atattgctct cgccgccgaa gtcgtaattc cacacgtgat cgaagatcac gtcacgggtc
     8161 agcacctgtc gcggatggcg catgaacaac tccagcagtt catactcctt ggcagtcagg
     8221 tcgaatgtct cgccgccgcg ccggccctgg cgggtgcccg tgtccagctc cagatcggcg
     8281 aaccgaaaga cctggggttg ggccggttgg gcgcgccgca acaaggcccg cacgcgagcc
     8341 atcagctcat cgaaagcgaa cggcttgacc agataatcat cggccccggc atccagcccg
     8401 gccacgcgat cgtcgatcgc ttccttggca gtcagcatca ggatcggcac atcactgacg
     8461 gcgcgcaaac gccggcaaac ttccagcccg tccatcccgg gtaacatcag atccagcagc
     8521 accagatcgg gcgggttatc cctggccagt tccagagcgg tgggaccgtc gggggccgcg
     8581 tcgacccgat agccttcgaa accgaggccg cgttgaagaa attgacggat gcgttcctca
     8641 tcttccacga ccagaattgt tgacgacatg gatgaacctc cagcttacta tcgcatcatt
     8701 atattatcct gcgtggctca ggcaaacccg gccgcccaaa agcgccaatc tcaccctttc
     8761 ttttaacaaa aattgttccc tgtgggcgcc tgggctacaa tccgcccgac tcgcgaatga
     8821 catgtcgcga gtcggctggg aagaggcatg gaagaactaa atcaactgag catcgctctg
     8881 gcaaccctga tgcaggaagc gtacccgcga ctcctggggt ttatgacctt catttcggcg
     8941 ctgggcaccg aggagttttt cctgctcgcc ctgccggtcg tctactggtg cgtcaataaa
     9001 caactcggcc gccagctggg atacttcttt ctggcctcgg cgctgatcaa caatgtcctg
     9061 aagaacctgt ttcgacagcc ccgtccgttc tggctgatgc cctccattca gcgcagcgat
     9121 attgaggggt tcggtcttcc cagcggtcac gtccagaatg tgacgaccgt catcctgatc
     9181 ctggccgccc acgtccgtcg cggctggttc tggctgtcgg gcctgttctt catcttcctg
     9241 atggggttga gccgcctgta tctgggagtc cactctctac aggatatcat cctcggcttt
     9301 gtcatcgggc tgcttctctt cggcattttc atcgcctggc aacagctctt ttacgaacgc
     9361 tacagcaatc aaatcctcgg ccgccgcctc ctgatcatga tcctggttcc catcatcctg
     9421 gcggccgtct acatcgggct attcttcatc gttggcccag ccgacaccag cgtatcatgg
     9481 gccgaataca tccccgccgc ggaactcaac ggctatgagg acatcgtttc ctcgctggcg
     9541 gccctgttcg ggttcggcat cggcattttg ctggagagca gtcgtatccg cttcatggta
     9601 gatggcccgc tatggttgcg cgccattcgt tatgtcctgg gtatgacagt ggcgctcggc
     9661 atctgggccg ggctgcgcgc cgttttcccg gctgagccac tctggctggc catgccgttc
     9721 cgctttctgc gctatttgct gctcctgttc tgggtgacct atttcgcgcc gtgggttttc
     9781 gtaaagttgc ggctggcttc cgccaacccg gagtctgagg tacgggttac cttttcgtaa
     9841 tcgccatacc catcaccttt ggggcctgag tgttgaaagt cgttcttggt gccctgcaag
     9901 ggttacctag cgcgaactaa atcgcggatc gcgctcaatc gtcaatcgca agccggtctc
     9961 cagatcgatc tccggccgga agccaagcaa ctcccgcgcc ttgctgatct cagccgccag
    10021 atggggcacg ccacccgact tttccgcgtt gtgaatgaca ttggcgcgct tgccggtcac
    10081 gcgctccata tgggcgataa gatcgaggat agttatttcc cggccgctgc cgatgttgat
    10141 gatcgagcga ttaacattct gcgcggtggc cgccgcgacg agagccgtca caacgtcatc
    10201 gacgtagaca tagtcgcgcg tctgtcggcc gtcgccaaag acaacaattg aaccgccgct
    10261 cagagcctgc ttcaggaagc gtggcacgac cggcgcgtgg gagacgggca agctctgtcc
    10321 cggcccgtag gcgttgaaga tgcgtaaggc aaccgtctcc attccccaca gcgcgccgat
    10381 cgtgtggata tactgctcgg ccgaccactt gctcacggcg taggggctgt cgggaaaagg
    10441 cgtgacgtcc tcgcggacag gttgccgagg ctggttgccg taaaccgcgc cggaagaagt
    10501 gaaaacgacg cggccgacgc ccgtgtcgcg catcgcctcc atcaagctga ctgtcccgcc
    10561 gacattgacc cgggcgtact cgcgtggatg gagaactgat tgcggcacgg agactcgcgc
    10621 cgccagatgg tagacgcaat cggcgctctg gagcaacgac cacaagagcg gaacgttatc
    10681 gacgtcccct ttggtgaatt cgacacgagg atcgaggcgc gccgcgtcac cgttgctcaa
    10741 gtcatccagg acgtgaaccc gatgatcatc ggccaccagc cgattggcca acgccgcgcc
    10801 cagaaacccg gccccgccgg taacaagaaa gcgcatcgat cgtcctcgtc ctcgatctcc
    10861 tgatactacc cctgctccac aaggtcaaac ggatcagggt caccggcata gatgcgatcg
    10921 aggatccctg tctcccgcgt ttcgatggta ttcaggaact cctccgcgcc cttcatgtgc
    10981 ttgaatgcgg cgtacccccg ctccagaaag ccctgaagct catgccagcc ggccagatgc
    11041 gccggggtat gtgccagccg caatgtcagg ccaatgaatg gcagacgtac gatcctgtca
    11101 acgctgcggc caattttggt gatcatgtca atttgtcgca cacgctcgtc atagttatca
    11161 cagatacgat atgcctcggc gtacgattgg ggcgtgatat gctccacgct cagctgatca
    11221 aaaagcgcat cggccagttt ggcgtcgagt tcatcggtaa attgatgaag ctcaatcgtc
    11281 tcggccagag tatggactac ccgctcgggc accgccttgc gcatggcatc ataggccttc
    11341 tggatatcgt aattgcgctg gctaaaatcc cgcggggcat agacgtcgct cagaaagaag
    11401 cgagtagcag gccgaaagcg cgggtgttcc agcagatcat gatgcgtcca ggccagtcgc
    11461 gtcgattgcc attcccgcaa cagcctgatt tccggcggta attcgcccgc ctcgaccttc
    11521 acgtgcttgc ctggccgcgc ctcgaccaga tgagtcagca actgcgccgc ctcgcgggca
    11581 gtcggtatcg ttgattcgga tgaatgtccg gtttctgtac tcatggatat gttcgccttc
    11641 gccggttcgc tacgaaatac gacacggtag catcaacaac ggcaacgctt ccattatcac
    11701 ccacacacct tacggatgcc aacgcatgag gcccttgcca agaaagcgtt atttcgatac
    11761 atttctaatt agtattttat ctaattagag gatcatcgaa atgactggta taagcaagga
    11821 agttctctca gaagaacaat ttgatcaatt actccgtttc tttcaggtat tcagcaacga
    11881 aagccggctc aagataatcg gccacctggc taacggcgaa aagaacgtgg gcgaattggc
    11941 cggcctgctg agagtgaagg aacctacggt gtcccatcac ctggcggaaa tgaaggccat
    12001 cggcctggtg aatgtgcgcg ccgaaggcac gatgcgcatc tatcggttga actccagggc
    12061 gctggaagcc atgagcaagg atatattcgc tcattccaat ctggcagcgc tcgtgaaaac
    12121 atcagagttg agcgaagacg agaagattct gcgcacctgg gtcaaggacg gccgtatcat
    12181 cgatttaccg gcccaggaaa agaaaaagca ggtaatcatc cactggctgg ctgatcgaat
    12241 tccccacgac cgccgctgga cggagcgcga gtttagcgaa tggctgacgc agtttaatga
    12301 ggattacgca acactgcgtc gttatttggt ggatagcggt tacatgaccc gcgagaaggg
    12361 tatttattgg cggacattaa aagcataaga atccgccggc atgggcaggc ggtttatcca
    12421 cacgcaccgc tgattcgatc cgactgtgtt ataataccct cgttgaatta tgctggccga
    12481 cgaggacgtg caacaatgat cgaagcaccc gctctcccca aacctgcgtc agaaatcatg
    12541 cccctggtgg ttaaatttcg gccgctctat cccatgacgc ccgacgattt ccaggagttc
    12601 tgcgcgctca atcctgattt ggtcgttgag ttaaccgctg aaggagaaat tgtctttatg
    12661 tcgccatccg gtgggaccac gggagcacgc aatgcgaaag tcatcatcgc tttagggcaa
    12721 tgggcaaacg aagatggacg tggcgtcgtc ttcgattctt cgaccggatt tgtattggcg
    12781 aacaaggcag tccgcgcgcc ggacgcagca tgggtacgcc ggagcagcct ggcgaaatta
    12841 acgccgcctc agaaagaacg attccttccg gtcacacctg atctggtggt tgagatcgcc
    12901 tcaccgagcg atcgggaatc cgaactccag cccaagatgg aagagtatat cgccaatgga
    12961 gtgcagctgg gctggttgat cttgccggag actcgcagcg tcatcgtgta ccgaccaggc
    13021 aagaaaggcg agttgctcag cgacatcgac tccatctcca gcgaccccgt cttgccgggt
    13081 tttactctcg atctgcgtcc tatttggatg ccggactttt aaaagaggga gctgcggatt
    13141 tcagagattt gcggattaat taggtcccgt agaaattgcg aacccgacat gatacaagtg
    13201 atattcggcc gcctggtgta acaacagcgg ccgttttctt tataacagga gcaaccccca
    13261 tgcacgacgt tctcatccgc aatagcctca tctacgacgg cagcggcaag ccgccctatt
    13321 cggccgatct ggccatcacc ggcgacacca tcaccgaaat cggccgcatc gacggccgcg
    13381 cccaactgac cattgacgcc gacggcctgg ccgtctcgcc cggcttcatc aatatgatgt
    13441 gctgggccaa cgagtcgctc atccacgatg gccgctcgca atccgacatc cgccagggcg
    13501 tgacgctgga aatattgggc gagggctggt cgatgggacc gctcaacgac gaactcaagg
    13561 cttacacgaa ggagctgcaa gg
//
LOCUS       DAOMQG010000052        13580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1948_length_13580_cov_11.128207, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000052 DAOMQG010000000
VERSION     DAOMQG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13580
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_13580_cov_11.128207"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="tmk"
                     /locus_tag="PLC06_04815"
     CDS             <1..627
                     /gene="tmk"
                     /locus_tag="PLC06_04815"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRO89799.1"
                     /translation="MFITFEGPEGSGKSTQIRLLAEFLRARGLSVEVVREPGGTPIGD
                     QVRHVLHDTANTAMSPEAEVLLYSASRAQLVSQRIRPSLAAGRVVLCDRYADSTMAYQ
                     GYGRGLDLGMLAALTEIATGGLKPDLTLLLDLDVSAGLSRRRVRGEEMNRLDLEAVAF
                     HERVREGYRLLANADPERWVRVQADRPVDVVAADIREAVLARLPVAAR"
     gene            901..1539
                     /gene="gmk"
                     /locus_tag="PLC06_04820"
     CDS             901..1539
                     /gene="gmk"
                     /locus_tag="PLC06_04820"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRO89800.1"
                     /translation="MENEDTVSDSLYSCDTYPLLLILSGPSGVGKDTVARRLIERRPE
                     NFYFVVTATTRPPRPDEVHGVDYLFVSNDEFAHMIETDELLEHAIVYNDYKGIPKQQI
                     RDALASGRDVILRVDVQGAHTVRSLVPNSVSVFLTTRTEQGLVDRLQHRKQDTAEGMA
                     LRTATARQEMKRIREFDYCVVNPEGQPDIAVDQVLSIIDAEHSRVNQEPVEL"
     gene            1678..2535
                     /locus_tag="PLC06_04825"
     CDS             1678..2535
                     /locus_tag="PLC06_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRO89801.1"
                     /translation="MWIKKFALLSILLLSLALVACGGTGATTPAATEAPAGAESGEAS
                     ALPDLGGREITVAVENAYLPFNYIDPATGEPAGWDYDAIRAICDLLNCKPVFTEAAWE
                     GMIQAVADGQYDMAADGITITDERAQIVDFSDGYINIDQRFLARIDEDRFDSIQAFID
                     NPELILGTQTGTTNFETASGILPADRIQAFEQFPFAVQALIAGDIDAVIIDETAGLGY
                     QGANADALKLVGDSLSSDQLGFIFPKGSDLVAAFNAAMVQLQANGTMATLAEKYFTDA
                     FTITYDDLE"
     gene            2701..3561
                     /locus_tag="PLC06_04830"
     CDS             2701..3561
                     /locus_tag="PLC06_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRO89802.1"
                     /translation="MTGEIQTNLNNNKDQDSRFSVREFPWWLVAIIGFLLLMLLLIMT
                     NPDYNRAFNFIKGGFGGIAGFQQGGLAGFIGTGITITIYTTLVAFAIALVIGLLAGLG
                     RVSTNVFVRNIAITYIEFIRGVPTLVLIFTVAFVIVPSVSSAIGISGQSISTNARGIT
                     ALAVIYGAFLAEVFRAGIESVPAGQTEAARSLGLNGRQAMRYVVLPQAVRNVLPALGN
                     DFIAMLKDSSLISVLAVRDITQMARLHAGSTFRFRESYLVLTFLYLSMTIALSLILRW
                     YEKRLRQNAR"
     gene            3551..4339
                     /locus_tag="PLC06_04835"
     CDS             3551..4339
                     /locus_tag="PLC06_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRO89803.1"
                     /translation="MQDNITETSPMTTNRVLGDDIIVIDNLYKIFGSVMAVCGFSLTV
                     RQGEVVVIVGPSGSGKSTVLRCINHLEVPSDGSIHVDGVHLEDKKTNINAVRAEVGMV
                     FQQFNLFGHLSVLDNITLAQRLIRKRNKQEAEEIAMEQLRRVGIPEKANAFPRQLSGG
                     QQQRVAIARSLAMNPKIMLFDEPTSALDPEMIKEVLDVMLDLARGGMTMVVVTHEMGF
                     ARSAADRVVFMDQGCVVEMAAPKVLFSDPKHERTKLFLSKIISH"
     gene            4432..5331
                     /locus_tag="PLC06_04840"
     CDS             4432..5331
                     /locus_tag="PLC06_04840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89804.1"
                     /translation="MDLSVIVVSYNTRQLLDECLSSLYAADAPDGGFEVIVVDNASVD
                     DSAAMVTAEYPQATLIVSDVNLGFSAANNRGTAVATGEYLLFLNSDTVVGRDALTKPL
                     AFMQENPRVGALTVRLVYPNGERDPDNHRGFPTPSNALFHFTGLSRLFPNSRRLNGYF
                     RNFEDFSKIHAVPVIAGSYMMVPRSLDRRLGGWDETYFFYGEDIDYCYRIYEAGYEII
                     YYPLVEVLHYKGASSGLRKESADIARPPKETRVKVARESVRAMELFYQKFYKDKYPRI
                     LTALVLAGIRLRGQVRILKHQLT"
     gene            5368..6678
                     /locus_tag="PLC06_04845"
     CDS             5368..6678
                     /locus_tag="PLC06_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HRO89805.1"
                     /translation="MAKIAFIGAGSTVFAKNLLGDILSFPELAGSTIYLHDIDEARLR
                     SSEIVANRVAGALGAKPRIVAGMDRRRALDGADYVITMFQIGGYKPSTVIDFEIPKKY
                     GLRQTIADTLGIGGIMRGLRTIPVLLDMCREMDELCPDAWLLNYVNPMAMNCWAVNRA
                     TRIKNVGLCHSVQGTAEQLARDIKVPIEEINYLSAGINHMAFYLRFERDGEDLYPLIR
                     QVAASDQVPDWNRVRYEVFRHLGYFVTESSEHFSEYVPWFIKRDRPDLTEKFNIPLDE
                     YITRCENQIAAWEMMRVYLEDESVELSVERTHEYGAYIIHSLETGTPRVVYGNVANNH
                     LIENLPVGCCVEVPCLVDANGVQPTHIGALPPQLAAAMQTNINVQALTVEAALTGNRE
                     HIYHAAMMDPHTAAELDLNQIHSLVDDLIAAHGDWLPDYHGNAA"
     gene            complement(6694..7434)
                     /locus_tag="PLC06_04850"
     CDS             complement(6694..7434)
                     /locus_tag="PLC06_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRO89806.1"
                     /translation="MSTYRVELPVFNGPLDLLLHLIDREELDITAVSLVQVTGQYLTQ
                     IRQMGDGQIEGLIDFISIGARLLLIKSRALLPRPAIVLTDGEEEEDPAEALLRQLRTY
                     KRFKNAAKWLDQRQQRGLRTYLRVAPPPRIEGHLDLSGVDAETLLEAMQAVLARVETM
                     EESLDVARPREITIEDQMGKFRHSLDRGRPFLFTDILTNLHDRTEISVSLLALLELIK
                     RREAQAFQTRMFGPIEITVAQQGPAHSP"
     gene            complement(7431..9050)
                     /locus_tag="PLC06_04855"
     CDS             complement(7431..9050)
                     /locus_tag="PLC06_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89807.1"
                     /translation="MSRIPQIVIGLAVAAFIGYLIVYLIYAVELFRFPFDYDQGEGFE
                     LMDTVYFSRGEWPYRDNDTYPFYSSNYPPLFHVVIVPLVWLFGPQYWTGRLVSFLGTI
                     ITAMAIGYAVARGRGQPDKQERRYWLAAISGLAFLASNYVYHVGPLFRQHMFMVMFET
                     LAVVLLAITVEREERDGRHYNGSLLGVMILLLAAGYTKQLAYATVAAVFIFLFLRNPK
                     RAILWAVPFAAVTGLIFVAINFATNGEWMLNTVTANLNPFVPGQAEGLFRQWFRLHTL
                     IIIVSAIYAVYQLYATRLSIYAIWFVVATVNSITAGKWGAGESYFATAIAAVCILSGL
                     AFHHVLDWAQSREWTSAAQARRVAIILPTTIGLLYLWQASLVFHMPTHTPLLAGIARA
                     LGQPTEVMIAPQTSCSAVRLPESIPYVDRAGVSLLGRPPNAADTAAGKQIAALIAKGN
                     TPAFGEDAGFNLFIGRDVVTNPTQLLNLYNNNAVDLTEMIRMLDEQKFDTVVLRAQFY
                     PGPVLDMIGQRYETTDLVQMNGFVYCVMRPR"
     gene            complement(9160..9357)
                     /locus_tag="PLC06_04860"
     CDS             complement(9160..9357)
                     /locus_tag="PLC06_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89808.1"
                     /translation="MVTKRQLGLLFILMGVGAIFAMFAIDLIGAGQFQGIGPAQRQAL
                     VVAGLIIFVGATLLPLGNRPA"
     gene            9445..10107
                     /locus_tag="PLC06_04865"
     CDS             9445..10107
                     /locus_tag="PLC06_04865"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRO89809.1"
                     /translation="MFVRLSATTRQQPTLTREKPDSGRPTSRLIGLLSLLSVLFVGWV
                     LWRYGVAADAVLRMPSWVADFLGLLTAAGLLTLVVLWGVVFWQYRRRQNNAGFQGMSI
                     DELYALSPKAFEHYVAELFERRGYTVEVRGRSGDLGVDLMLSRNDGRRAIVQCKRYRH
                     PIGPEIVRELFGTMLHERVVHGFLVTTAEISDGARSWAADKPITLIDGAALAQLGSTP
                     TR"
     gene            10139..10759
                     /locus_tag="PLC06_04870"
     CDS             10139..10759
                     /locus_tag="PLC06_04870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRO89810.1"
                     /translation="MTTQLIFIRHAQSTWNEVRRWQGHANPPLAESGRRQARLLAQRL
                     SKWKIDCLYASDLLRAAETAEIIGAAIGLKPIHDSLWRERGFGALEGLTAEEIALQFP
                     DAWASRMVGPISGVPGAEPQADVIARSLAGCSNLLTQHQDQTVVVVSHGGMILTTLVH
                     LLGLPPSGHSLLVVGKNTSISRVAIENGHARLAGLNDTAHLEMLDT"
     gene            10807..12192
                     /locus_tag="PLC06_04875"
     CDS             10807..12192
                     /locus_tag="PLC06_04875"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRO89811.1"
                     /translation="MFNPPSPPRYVDYRRHVDELVTAGIGAADSGLAVSGFLKKEGRT
                     LSIGREPEIEPIDLDQGRVFLVASGKAAVPMARAALRVLGDDLEAGIIITKDIGQGEV
                     GVAREDEFAGRPVKIIAAGHPVPDERGIDGTKAVVEMLSAARREDVVLCLISGGTSAL
                     LTQPLMPLEDWRKLVELLLKSGCTINEFNIVRRSVDRIKGGGLARAASPATCYGLILS
                     DVVGNPLNMIGSGPTVPDEDLVVSAVQVLGRYDIARQLEREEWGRLALALNQARYLHK
                     APRPSVRNFIVGDVRGAATAVLVAAIRLGFVGQVLTTHLDGQAREIGRLTAGIARDLP
                     PGQCLIMGGETTVTIRGKGVGGRNQELALSAAIALERQPHVAVFSFSTDGEDGPTPAA
                     GAVVTGETARLARSYGINPMAHLMDNGSYSFFARLDEVSRGRVEPHLIVTGPTGTNVN
                     DLVVILSYGDA"
     gene            12256..>13580
                     /locus_tag="PLC06_04880"
     CDS             12256..>13580
                     /locus_tag="PLC06_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRO89812.1"
                     /translation="MTVKFGTDGWRAVISEEFTFDNLRLVAQAIADFVLEENASDPSV
                     VIGFDTRFLSDRYAAEVACVMAANDIRALLARADTPTPAISYAVANRNATAGVMITAS
                     HNPPRYNGVKLKASYGGSASPEQHRRVEGFLEQNASAARKPRLMALEDAIRADKIVKF
                     DPAWAYYEHLGTLVNLDIIHAGELRVVHDSMYGAGRRTISEMLMRTRTDVLNLRDEMN
                     PGFGGVHPEPIARHLSLLASVMQNSNRHAGLATDGDADRIGAVDARGQFVDPHRIFGL
                     ILHYLLEKRGLRGRVVRTVSTTRMIDRIAADFGLIVVETPVGFNHISDLMIADNVVMG
                     GEESGGMSIQGHIPEGDGVLMGLLLLEVLAEAHKPLHVMVDELLAAYGPAQYARTDLK
                     LSRPVAKSALTRKLVETAPDAISGVEVEEVRTSDGIKYYLADGSWLLIRP"
BASE COUNT         2848 a         3561 c         4074 g         3097 t
ORIGIN      
        1 atgtttatca cgttcgaagg gccggaaggc agcgggaaat cgacccagat tcgattgctg
       61 gccgagtttc tgcgcgctcg tggactctcc gtcgaagtag tgagagagcc gggggggact
      121 ccgatcggcg atcaggttcg gcatgtattg cacgatacgg ccaacaccgc gatgtcgccg
      181 gaggcagagg tattgcttta ctcggcctca cgagcccagc ttgtgtcgca acgcattcgt
      241 ccctcgctgg ccgccggccg ggttgtcctg tgcgatcgtt atgccgatag cacgatggcc
      301 taccagggat atggccgggg gcttgatctg ggcatgttag ctgcattgac cgaaattgcc
      361 acaggtggtc tgaagcctga tttgactttg ttgctcgatt tggatgtatc ggcgggatta
      421 tcgcgccgac gcgttcgggg cgaggagatg aatcggctcg atttggaggc tgtggctttt
      481 catgaacgcg ttcgcgaagg gtatcgattg ctggccaacg ccgatccgga gcggtgggtg
      541 cgggttcagg ctgaccgacc tgtggatgtg gtagcggccg atattcgcga ggccgttctt
      601 gcgcgcttgc cggttgctgc ccggtaaaat aatgttcatc atccctggtt gatgggcgaa
      661 aaccattgcc ttgcttgatg gtagtgccga cctttttcgg aaaaaccggc accgggcact
      721 caatggattt ggcaggttcc caatactctg atcagctcat gcgatcgtcc aattcctcgc
      781 tcatttgaca acccaattca ccattttggc aattgctgct acaattgttc cattatatgg
      841 agcgacttga agattttatc ggtcgcgcca tcgaacaata tgatgaatat aagcgcaatt
      901 atggagaatg aagatactgt gtcggatagc ttgtatagct gcgataccta tccgctcctt
      961 ctgatactat ccgggccgtc cggcgtgggt aaggatactg ttgcccggag attaattgag
     1021 cgccgccccg agaacttcta ttttgtcgtc acggctacga cgcggccgcc gcgccccgat
     1081 gaagttcatg gcgtggatta tttgttcgtt agtaacgatg agttcgctca catgatcgag
     1141 accgatgaac tgctggaaca cgccatcgtc tataacgact ataagggaat acccaagcaa
     1201 caaattcgtg acgccctggc gagcggccgc gacgtcatcc tgcgcgtgga tgtccagggc
     1261 gcacacacgg tgcgcagcct cgttcccaat tccgtttccg tttttctgac cacgcgaacg
     1321 gaacaaggac tcgtcgatcg cttgcagcat cgcaagcaag atacggccga gggaatggcc
     1381 ctgcgcacgg cgaccgcacg acaggagatg aagcggattc gggagtttga ttactgcgtg
     1441 gttaaccccg agggacagcc ggacatagcc gtcgatcaag ttttaagtat cattgatgcc
     1501 gaacatagcc gcgttaatca ggaaccggtg gagctttagc tgaaaacacc ttttctaatc
     1561 gcgaaaatat gttaaaaccc tgttgtgttg cgagggattt gttgtctttg gccgaaacaa
     1621 gggaagcaaa ttaccgcgtg tgttggcaac tgttttttat tgagaaggag aagagatatg
     1681 tggattaaga agtttgctct attgtccatc ctgttgttgt cgctggcgct tgtggcctgt
     1741 ggcggcactg gggctacgac gcccgcggct acggaagcgc cggccggggc ggaatcaggt
     1801 gaggcgagtg cgttgccgga tctgggtggc cgcgagatca ccgtcgccgt agagaacgct
     1861 tatttgcctt tcaactacat cgatcccgcg accggtgagc cggcaggctg ggattatgat
     1921 gcgattcggg cgatttgcga cttgttgaac tgcaagccgg ttttcactga ggcggcatgg
     1981 gaaggcatga tccaggccgt ggccgatggc caatatgaca tggccgccga cggcatcacc
     2041 atcactgatg agcgtgccca gatcgttgat ttctccgatg ggtatatcaa catcgaccag
     2101 cgtttcctgg ctcgcatcga cgaggatcgg ttcgattcga ttcaggcttt catcgataac
     2161 cccgaattaa ttctgggcac ccagacgggc acgaccaact ttgagaccgc ttccggcatt
     2221 ctgccggcgg atcgcatcca ggctttcgag cagttcccgt tcgccgttca ggccctcatc
     2281 gccggtgata ttgacgccgt gatcatcgat gagactgccg gcctgggcta tcagggcgcg
     2341 aatgccgacg cgttgaagct tgttggcgat tcgctctcca gcgatcagct gggcttcatc
     2401 ttcccgaagg gcagcgatct ggtggccgcg ttcaatgccg cgatggttca attgcaggct
     2461 aacggcacga tggcgacgct ggctgagaaa tatttcaccg acgccttcac cattacctac
     2521 gatgaccttg agtagtttct gagagcggtc gttgccagac aacggccatg ttttcacggt
     2581 tgcattgcaa aagacgccac agatttcttg tggcgtcttt tcatttggcg cagtcagtgt
     2641 atcattgcgc cgtctgccaa aatattggtg tggtgggaac gttaaaatgg tggaggtttg
     2701 atgacaggcg agatacagac caacctgaac aataacaagg atcaggacag ccgattcagt
     2761 gtgagagaat tcccgtggtg gctggttgcc atcatcggtt tccttctgtt gatgctgctg
     2821 ctcatcatga cgaacccgga ttacaaccgg gcattcaact ttatcaaggg aggcttcggc
     2881 ggcatcgctg gcttccagca gggaggactg gcgggtttca tcgggaccgg catcacgatc
     2941 acgatttata ccactctggt cgccttcgcc attgcgcttg tcatcggact tctcgccggg
     3001 ttaggtcgcg tcagcacgaa tgttttcgtg cgtaacatcg ccatcaccta catcgaattt
     3061 atccgcggcg tgccaacgct ggtgctcatt tttacggttg cgttcgttat cgtgccgagc
     3121 gtgtcgagtg caatcggcat ttccggtcag agtatctcga ccaatgcgcg tggtattact
     3181 gcgctggcgg tcatttatgg cgcgttccta gctgaggttt ttcgtgcggg tatcgaatcc
     3241 gtgccggccg ggcagacgga ggcagcgcga tcgttggggc tgaatggtcg ccaggccatg
     3301 cgttacgtcg tcctgccgca ggcggtccgg aatgtattac ccgccctggg aaacgatttc
     3361 atcgccatgt tgaaggactc atccctgatc tctgtattgg ctgtccggga tatcacccag
     3421 atggctcgtc tccacgccgg cagtaccttc cgctttcgcg agagctatct cgtcctgacg
     3481 tttctctatc tatccatgac catcgctctg agcttgatcc tgcgttggta tgagaagagg
     3541 ttgcgacaaa atgcaagata acatcactga aaccagtcca atgacgacca atcgtgttct
     3601 gggtgacgac attatcgtta tcgataatct gtacaagatt ttcggcagcg tgatggcggt
     3661 ctgtgggttt agcctgaccg ttcgtcaagg tgaggttgtt gttatcgttg gccccagcgg
     3721 ttcaggcaaa agcacggtat tacgctgcat caatcatctt gaggtgccgt ccgatggctc
     3781 gatccatgtc gatggagtgc atctggagga caaaaagacc aatatcaacg cggtccgggc
     3841 ggaggttggc atggtgttcc agcaatttaa tctgttcggt cacctgtccg tgctggataa
     3901 catcaccctt gcccagcgac ttattcgcaa gcgcaacaag caggaagctg aagaaatcgc
     3961 catggagcag ttgcggcgcg tcggcattcc ggaaaaggct aacgcatttc cccgacaatt
     4021 gtccggcggg cagcaacagc gtgtagccat cgctcgctcg ctggcgatga atccgaagat
     4081 catgttgttc gatgagccta ccagcgcact tgacccggag atgattaaag aggtgctgga
     4141 cgtcatgctc gatctggcgc gcggcggtat gacgatggtc gtcgtgacgc acgagatggg
     4201 ttttgcccgc tcggccgccg atcgggtcgt gttcatggat cagggctgtg tggtggaaat
     4261 ggccgcacct aaggttctgt tcagtgatcc caaacacgag cggaccaagt tgttcctgag
     4321 caagatcatc agccactagc tgtggcggtt actcatggcc catgttggtg ttggtctttc
     4381 gcagggataa ggtggggtag cctgcccgaa aaggggtaaa atcgatcctg tatggatctt
     4441 tcagtcattg ttgtgtccta caacacgcgt cagttgctgg atgaatgcct cagctctctt
     4501 tatgcggccg atgcgccgga cggtggcttt gaggtcatcg tcgtcgacaa cgcctctgtt
     4561 gatgacagcg cggcgatggt gacggccgag tatccacaag caaccctgat tgtcagtgat
     4621 gtgaacctcg gcttttcggc ggcgaataat cgtggtacgg ccgtagcgac gggagagtat
     4681 ttgctgttcc tcaattccga tacggtagtc ggacgggatg cgctcacaaa gccactggca
     4741 ttcatgcagg agaacccgcg agtcggggcg ctgacggtgc gactggtata tccgaatggg
     4801 gagcgagatc ccgataatca tcgcgggttc cccacgccgt ccaacgcctt gtttcacttc
     4861 acaggcctca gccggctgtt ccccaacagc cgacgattga acggttactt caggaacttt
     4921 gaggacttct cgaagattca cgccgtaccc gtgattgccg ggtcgtatat gatggtgccg
     4981 cgctctcttg atcgccggct cggtggatgg gatgagacgt acttttttta cggcgaggat
     5041 atcgattact gctatcgcat ttatgaggcc ggatacgaga ttatttatta ccccctcgtc
     5101 gaagtgttgc attacaaagg agccagctcc ggcttgcgca aagagtcggc cgacatagcc
     5161 cgaccaccca aagagactcg cgtcaaggtt gctcgcgaat cggtccgcgc catggagctt
     5221 ttctatcaga aattctacaa agacaagtat ccgcgtatct tgacggctct ggttcttgcc
     5281 gggattcgac ttcgcgggca ggtgcgcatt ctaaagcatc aattgacata atgtaaatta
     5341 ttcttgcttc catatggaga ggtaactgtg gcgaagatag catttatcgg tgcgggaagt
     5401 acggtttttg ccaagaactt gctgggggac atcctcagct tccccgaatt ggccggatcg
     5461 acgatctacc tgcatgatat tgacgaagct cgtttgcgca gctccgaaat tgtagccaat
     5521 cgggtggccg gcgcgttagg agcaaagccc cggatcgtcg ccggtatgga tcgtcgtcgg
     5581 gcgctcgatg gggcggatta tgtgatcacc atgttccaga tcggggggta taaaccttcg
     5641 acggtgattg atttcgagat tcccaagaag tatggcttgc ggcagaccat cgccgacaca
     5701 ctgggtatcg gtggaatcat gcgtggcttg cgaaccattc ccgttctgct ggatatgtgt
     5761 cgcgagatgg atgagctatg cccggatgcc tggttgctca actacgtgaa ccctatggcg
     5821 atgaactgtt gggccgtaaa tcgggcaacg cgaatcaaga atgtcgggtt gtgtcatagc
     5881 gtgcaaggga cggccgaaca gttggcccgt gacatcaagg tgccgatcga ggagatcaat
     5941 tacctcagcg cagggatcaa tcacatggct ttctatctgc gtttcgagcg cgacggtgaa
     6001 gacctgtatc ccttaattcg ccaggtcgcc gcgagcgatc aggtgccgga ctggaatcgc
     6061 gtgcgttatg aagtgttccg gcatctgggg tatttcgtca ccgaatcgag cgaacatttt
     6121 agcgaatacg tcccctggtt catcaaacgc gatcgccccg acctgactga gaagttcaat
     6181 attccgctcg atgaatatat tacccggtgt gagaatcaga ttgccgcctg ggaaatgatg
     6241 cgcgtctatc tggaagatga gtcggttgaa ctgtccgtcg aacgcaccca cgagtatggg
     6301 gcctatatca tccatagcct ggagaccgga acgcctcgcg ttgtttacgg caacgtggcc
     6361 aataaccatc taatcgagaa tttgccggtt gggtgttgcg tcgaggttcc ctgtctggtc
     6421 gatgcaaatg gcgtccagcc gacccacatc ggcgccttac cgccgcagct ggccgcagcc
     6481 atgcaaacga atatcaatgt gcaggctttg accgttgagg ccgccttgac cggtaatcgg
     6541 gagcatatct accacgcggc gatgatggat ccgcacacgg cggccgaact ggacctgaac
     6601 cagattcaca gccttgtcga tgatctgatc gcggctcacg gcgactggtt gcccgattat
     6661 cacgggaacg ccgcgtaact tgttcggctg tctttaggga gaatgcgccg ggccttgctg
     6721 agcgacggtg atctcgatcg gcccaaacat gcgtgtctgg aaggcctggg cctcacgccg
     6781 tttaataagc tccaatagtg ccagaaggga aaccgagatc tctgtccggt catggagatt
     6841 cgtcagaatg tcggtgaaca gaaagggtcg gccgcgatcc agggaatggc ggaatttgcc
     6901 catctgatcc tcgatagtga tctcgcgcgg gcgagcgaca tccagcgatt cctccatcgt
     6961 ctccacccgg gccagcacag cctgcatcgc ttccaggagc gtctcggcat ccacgccgga
     7021 gagatcaaga tgcccttcga tgcggggcgg tggggctacc cgcaaatagg ttcgcagacc
     7081 gcgctgctga cgctgatcga gccacttggc ggcgttcttg aagcgcttat acgtccttaa
     7141 ctgacgcaag agcgcttcag cgggatcttc ttcctcttcg ccgtccgtga gaacgattgc
     7201 cggtcggggc agcaaagcgc gactcttgat cagcaacaat cgcgcgccga tgctgatgaa
     7261 gtcgatcaat ccctcgattt ggccatcgcc catctgccgt atttgagtca gatattgacc
     7321 tgtcacctgg accagcgaga cggccgtaat gtccagctct tctcgatcga ttagatggag
     7381 caagaggtcc agcgggccgt taaagacggg gagttcaacc cggtaggtgc tcatcggggg
     7441 cgcatcacac agtagacaaa gccgttcatt tgcacaagat ccgtcgtctc atatcgttga
     7501 ccaatcatat ccaaaacagg gccgggatag aattgagcac gcagaacgac tgtgtcaaat
     7561 ttctgttcgt ccagcatcct gatcatctcg gtcagatcga cggcgttgtt gttgtagagg
     7621 ttgagcagtt gggtagggtt ggtcaccaca tcgcggccga taaacagatt gaagcccgcg
     7681 tcttcgccga aggcaggggt gttgcctttg gcgattagcg cggcgatctg ttttccggcg
     7741 gctgtgtcgg cggcgttggg tggcctgccc agcaacgaga caccggcccg atcgacatag
     7801 gggatgctct ccgggagccg aacggccgaa catgacgttt gtggcgcgat catgacttcg
     7861 gtcggctgcc ccaatgcccg tgcgattccg gccagcagcg gtgtgtgagt gggcatatgg
     7921 aacacgagac tcgcttgcca caggtagagc aagccaatgg tagttggcag gataatggcg
     7981 acccggcgcg cctgggcggc cgacgtccat tcccgcgact gtgcccaatc gagcacatgg
     8041 tgaaaagcca ggccggacag gatgcagacg gcggcgatgg ctgtggcgaa ataggactca
     8101 cccgcgcccc acttgccggc ggtgatggag ttgaccgtag cgacaacaaa ccagatggcg
     8161 tagatcgaca gtcgggtagc gtatagctga tagacggcgt aaatggccga gactatgatg
     8221 atgagcgtat gcagccggaa ccattggcgg aaaaggcctt ctgcctggcc gggaacgaat
     8281 gggttaaggt tggccgttac cgtattgagc atccattcgc cgtttgtggc gaagttgatc
     8341 gccacaaata tcagcccggt gacggctgca aaaggcactg cccagagtat tgctcgcttg
     8401 ggattgcgca ggaacaggaa gatgaagaca gccgcgaccg tggcataggc cagttgcttg
     8461 gtgtagccgg cggccagcag caggatcatc acccctagca atgacccgtt atagtggcgg
     8521 ccgtctcgct cctcgcgctc gacagtaatc gccagcagga cgaccgccag cgtttcgaac
     8581 atgaccatga acatatgttg ccggaagagc gggccgacat gatagacgta gttcgacgcc
     8641 aggaatgcca ggcccgagat ggccgccagc cagtaacgac gctcctgttt gtcgggttgg
     8701 cctcggccgc gggcgacggc atagccgatg gccatggccg tgataatcgt gcccaggaag
     8761 ctcaccaggc ggccggtcca atattggggg ccaaacagcc ataccagcgg gacgatgacc
     8821 acatggaaca gaggtggata gttggaggag tagaacgggt aggtatcgtt gtcgcggtat
     8881 ggccattcac cccgactgaa gtacaccgtg tccatcaatt caaaaccttc cccctgatca
     8941 taatcgaagg gaaagcgaaa tagctcgaca gcatagatga gatagacaat gaggtagccg
     9001 atgaatgcag caactgccag accaattact atttgtggaa tccggctcaa agctctttgc
     9061 cggaaaggtt ggttggttat ggagttcgta gaggacatgg tggatagccg gctcgaacga
     9121 tatgggggat tgttatgctc ctcattcagg ccatcaggtt taggcggggc gattccctaa
     9181 cgggagcagg gtcgctccga cgaaaataat cagcccggcg acgacaaggg cttggcgctg
     9241 ggccggcccg atgccctgga attgacccgc gcctatgaga tcaatggcga acatagcgaa
     9301 gatggctccc actcccatga gaatgaataa cagccccagt tgacgttttg tcaccatatt
     9361 gatggcaact ataacactgg cggtgttctg aatcaatttg agcatctccg ccggtcgaca
     9421 tacaataccg tccggtcaat cattatgttt gtcagactat ccgccacgac acggcaacag
     9481 ccgaccctca ctcgcgagaa gcctgattcc gggaggccga cctcgcggct tatcgggctg
     9541 ctctccctgc tgtcggtctt gtttgtgggg tgggtgttat ggcgatacgg tgtggcggcc
     9601 gatgctgtct tgcggatgcc gtcatgggtt gccgattttc ttggcttact gacggccgcg
     9661 ggcttgctga cgttagttgt tttgtggggc gtcgtatttt ggcagtatag gcgacgccaa
     9721 aacaatgccg ggttccaggg aatgtcaatt gacgaacttt atgccctcag cccaaaggcc
     9781 tttgagcatt atgtggccga actattcgag cggcgcggat atacggtgga agttcgcgga
     9841 cgctccggcg atctgggcgt tgacctgatg ctctcccgaa atgacgggcg gcgagcgatc
     9901 gtccagtgca agcggtatcg tcatccgatc gggcctgaaa tcgtgcgcga attattcggg
     9961 acgatgcttc atgagcgcgt cgtccacggc ttcctggtga cgacggccga gatttcggac
    10021 ggagctcggt catgggcggc cgataaaccg atcacgctga ttgatggtgc tgccctggcc
    10081 cagttaggct cgaccccaac ccgataaata tccccccttc gcgatcactg gataatccat
    10141 gactactcaa ctgattttca ttcgccacgc acaaagcacg tggaatgagg tacgacgttg
    10201 gcaggggcac gccaacccgc cattggcaga aagcggccgt cgccaggcgc ggctgctggc
    10261 ccagcgtctc tcaaagtgga agatcgattg cctctatgcc tcggatcttc ttcgagcagc
    10321 cgagacggct gaaatcatcg gcgctgcaat cggcctcaag cccatccacg attctctatg
    10381 gcgcgagcgc ggttttggag cgcttgaggg gctgacggcc gaagaaattg cccttcaatt
    10441 tcccgatgcc tgggcttcac ggatggtagg gccgatctcc ggtgtgcccg gtgccgagcc
    10501 gcaagccgac gtaatagctc gctcattggc cggttgctcg aacttgctga ctcagcacca
    10561 ggatcagaca gtggtggtcg tgagccacgg gggaatgatt ctcacgacat tggttcatct
    10621 cctgggcttg ccgccatccg gccattcatt actggtcgtc ggcaaaaaca catcgattag
    10681 tcgagttgcc attgaaaacg ggcacgcccg gttggccggt ttgaatgaca cagctcatct
    10741 tgaaatgctg gatacctgac tgatgggcgc ccgtgctccc cgtgctatac ttcctgtgtg
    10801 cccataatgt tcaatccccc gtctccgccc aggtacgttg attatcggcg acacgttgat
    10861 gaactggtca ccgcaggtat tggcgcggcc gattccgggt tggcggttag cggctttcta
    10921 aagaaagagg ggcgaacgct ttccatcggt cgcgaacccg agattgaacc tattgacctt
    10981 gatcaggggc gagttttcct tgttgcttcg ggcaaggcgg ccgttcccat ggcgcgcgcc
    11041 gccttacgcg tgctgggcga tgatctggaa gcgggcatca tcattacaaa ggacatcggg
    11101 caaggagaag ttggagtggc cagggaagat gaattcgccg gcagaccggt gaagatcatc
    11161 gctgccggtc atcctgtgcc cgatgagcga ggcatagatg gaacgaaggc tgttgtggag
    11221 atgttgtcgg ccgcgcgtcg ggaagatgtg gtgctatgcc tgatctccgg cggcacatcg
    11281 gcgctattga cccagccgtt gatgcctttg gaagattggc gcaagctggt ggaattgctg
    11341 cttaagagcg gctgcaccat caacgaattt aatattgtgc gtcgatcggt cgatcgcatc
    11401 aagggcggcg gactggccag agctgcaagc cctgcaactt gttatggcct tatcctctcg
    11461 gatgtagtcg gcaaccccct taatatgatt ggctccggcc ccacggttcc cgacgaggat
    11521 ttggtcgtca gcgccgtgca agtgctgggt agatatgaca ttgcccggca actggagcgt
    11581 gaggagtggg ggcggctggc gttggccctc aaccaggccc gctatttgca caaagcgccc
    11641 cggccgtcag tgaggaattt tattgttggc gatgtgcgtg gtgcggcgac tgcggttttg
    11701 gttgcggcga ttcgcctggg attcgtgggt caggttttga cgacccatct ggatgggcag
    11761 gcgcgggaga tcggccgtct gacagccggt atcgcccgtg atttacctcc tggccaatgc
    11821 ctgatcatgg gcggcgagac gaccgtgacg atacgcggaa aaggtgttgg cgggcgaaac
    11881 caggagttgg ctttatcggc tgcaatagct ctggagcgac agccccatgt ggccgtgttc
    11941 agcttttcca ccgatgggga ggatgggccg acgccggcgg caggcgcggt cgtaacgggt
    12001 gagactgccc gattggcgcg atcctatgga attaacccta tggctcacct gatggacaat
    12061 gggagctact cgttctttgc tcggctggat gaagtgagcc ggggcagggt ggagcctcat
    12121 ctcatcgtca cagggcctac agggactaac gtcaacgatc tggtcgttat cctctcatac
    12181 ggagacgcat aaaagccgat aatccggcgc atcgtaccac gaataagacc ccatctcggg
    12241 tattgctgga gaactatgac ggtaaaattt ggtaccgacg gctggagagc cgtcattagt
    12301 gaagagttta catttgacaa tctgaggctc gtggcgcaag ccatcgccga ctttgtgctt
    12361 gaggagaacg cctctgatcc aagcgtcgtt atcggtttcg acacgcgatt tctttcagat
    12421 cggtacgcgg ccgaagtagc ctgcgtcatg gcggcgaacg acatacgcgc gctgttggct
    12481 cgcgccgata cgccgacgcc ggcaatcagc tatgctgtcg ccaatcgcaa cgctaccgct
    12541 ggggtaatga tcactgccag ccataacccg cctcgctata atggtgtgaa actgaaggct
    12601 tcatacggtg gcagcgccag ccccgaacag caccgtcggg tagaaggatt tctggaacag
    12661 aacgcatcgg ccgcccgcaa accgcgcctg atggctctcg aagacgccat ccgcgccgac
    12721 aagatcgtga agttcgatcc cgcgtgggcc tattatgagc atctggggac gctggttaat
    12781 ctggacatca ttcacgcggg agaattgcgt gtcgtccatg attccatgta tggcgccggg
    12841 cgacggacga ttagcgaaat gctgatgcga acccgaaccg atgtccttaa tctgcgagat
    12901 gagatgaacc ccggctttgg tggcgtccac ccggagccta tcgccagaca tttgagcttg
    12961 ttggcgtcgg tcatgcagaa cagcaatcgg catgccggcc tcgcgaccga tggcgacgcc
    13021 gaccggatcg gtgcggtgga tgctcgtggt caattcgtcg atccacatcg tatctttggc
    13081 cttatccttc attatctatt ggagaagcga ggattgcgcg gtcgggtagt gcgcacggta
    13141 tcgacaactc ggatgataga ccgaattgcg gcggatttcg ggctgatagt tgtagaaact
    13201 ccagtggggt tcaatcacat atctgatctc atgatcgccg ataatgtggt aatgggagga
    13261 gaggagtccg gaggcatgag cattcagggg catatccctg aaggcgatgg tgtcctgatg
    13321 ggacttctgt tgctcgaagt gctggccgag gcccataaac cattacatgt gatggtggat
    13381 gagcttctgg cggcttatgg tcccgcccaa tatgcgcgaa ccgacttgaa actgtcgcga
    13441 cccgtcgcca aatcagccct gacgcgcaag ttggtcgaaa ctgctccgga tgcaatctca
    13501 ggcgttgagg ttgaggaagt gcggaccagc gatgggatca agtattatct ggcggatggt
    13561 agctggttgt tgatccggcc
//
LOCUS       DAOMQG010000053        13491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1961_length_13491_cov_10.816538, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000053 DAOMQG010000000
VERSION     DAOMQG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13491
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_13491_cov_10.816538"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1463
                     /locus_tag="PLC06_04885"
     CDS             81..1463
                     /locus_tag="PLC06_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRO89813.1"
                     /translation="MIEGYFDRLTDLLLNKNLRQANPDDEAARAARAHTLTTLRNLAP
                     EDRGQVVRFLYESALLDAAEPIVDLQAADLSGMTLSQVQMPAVNLRNVHLAGAQLGGV
                     VMPSSDLRETNLGDADLAKADLSESYLRGAHLRGATLAEANLRGAILTKAVFVEANLR
                     GADLSDANLSGADLSRADLTGANLKGAKLNNAIFAEALLEDTDVSLANLNRSNLSGVV
                     GNRANFSGANLAGTQLVGAKLNGAVLGGAILSEADLTDAEMREANLNLANLRGAILTQ
                     AILAGANLRDAIMGRVSLSGADLSGSDLSRANLSIANLSRANLNRANLEEAGLSGADL
                     RAAQLRGAILRGAILRGAILGDADLSRADLTKANLRWANLNNANLTGADLTEVDLTDA
                     EVSPQTLAKARSVSKVDRPDTSHFESAPAVIAPGQSGPLPASRTPSYQRPSDQPDGKP
                     KRSLEEKSNK"
     gene            complement(1491..2486)
                     /locus_tag="PLC06_04890"
     CDS             complement(1491..2486)
                     /locus_tag="PLC06_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRO89814.1"
                     /translation="MNLIPFNQTGLKVTPIGLGLAALGRPGYINLGHASDLSHDYDMA
                     SMESHAHSVLDTAWESGIRYFDAARSYGQAESFLSTWLRSRGIQSSEVVVGSKWGYTY
                     TAGWRVAAQKHEVKDHSLAVFKRQIGESLGLLGDYLDLYQIHSATLDSGVLDDWKVLE
                     ALGDMRDGGMVVGLSLSGPNQARTLRKAMTIKVGNKPLFGSVQATWNLLAREATEALN
                     EAHQSGMGVIVKEALANGRLTARNDDPDFASRKKLLKQVATELNTTIDALALGFVLAQ
                     PWADIVLSGAAQSEHLLSNIHALDITWTNALKERLASLVEPAEQYWATRSELDWN"
     gene            complement(2532..3155)
                     /locus_tag="PLC06_04895"
     CDS             complement(2532..3155)
                     /locus_tag="PLC06_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HRO89815.1"
                     /translation="MTPAQSDSPKTAFIICGALAREVTAIIKRHQWQVDLFGIPAIAH
                     MYPERIGPAVEQKYLALRMDYDRILVVYGECGTKGSLDELLNIYGLQRINGPHCYEMY
                     GGQKFADLMDEEPGTFFLTDFLVRGFHGTVWHGLGLDRFPELQEEYFRNYRRLIYLAQ
                     TVDPMLQEKAQEISTRLKLPLEIIQSGYGPFEERLIAFMEQQDVAIS"
     gene            3477..4982
                     /locus_tag="PLC06_04900"
     CDS             3477..4982
                     /locus_tag="PLC06_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89816.1"
                     /translation="MTNTPSGNESAEIQTGAEEASETQSGRGGTLRVAMQPVVNIDPI
                     AISSDSEVLVANHVYDYLIDIDHLSNPVPRLATGWDISDDGLTYTLPLAEDVTWHDGS
                     EFSAADVVWTFERLRTTEGAPTFDLYSNIASIEATGDLEVTFKLTKPNPFFLYDLSDN
                     HALILKAGTTDADTNFNGTGPFVVTNYRPEERIEMVANENYFIPEQPKLSGLQIIFFN
                     DDTVAVEALRGGQIDFTMRMSVALFESLKEEPGIITMDIPTNGFDLIRLRSDRAPGND
                     PRVIQALKLATDREAIFNVVQQGYGALGNDSPIGPLYDAYHDETAIPPARDVEAARAL
                     LADAGYGDGLKITLHTPDTGNRPDLAAVLKEQWSEVGVDVEISVEPESVYYADDGWLE
                     VDLGITGWGSRPYPQFYLDVMLVSDAIWNESHFSDEEFDSLAAVAGTTLDEKERIQAY
                     ADIQHLLIERGPIIIPYYFAQFGAINDQFQGLNLKAFAGRTDFRTVTFSGN"
     gene            5059..5892
                     /locus_tag="PLC06_04905"
     CDS             5059..5892
                     /locus_tag="PLC06_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89817.1"
                     /translation="MRAINVSSVLRELYARRTTALGTTIFLLFLFVAMFAPLFAPYGL
                     NEQIYSDARQAPSTAHWFGTDQLGRDVFSRVLLGAKDILTLAGLGTALAVASGTLFGL
                     AAGYWGGTFDEILMRIFDGLLAIPALLLALLLLGTLGASRNSILIVIVLLYTPIVARV
                     VRSVVLGVRSKGFVEAARLQGEPTGRILFREILPSVVPTLTVEAALRFSYAIFLVASL
                     GFLGVGVQPPSPNWGLMVNEARPHVSQLPWTMYYPAGAIAIVVIGVNLMADGIKRIVR
                     T"
     gene            5906..>6705
                     /locus_tag="PLC06_04910"
     CDS             5906..>6705
                     /locus_tag="PLC06_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89818.1"
                     /translation="MLNIQGLSVAYEQNGVLLPAVRDFSLSLAKGQTYGLVGESGSGK
                     TTVAMAILGYLRGEGRITGGRIEFEGRNLLTLPQSERIKVLGKSIALVPQDPMSSLNP
                     SMRVGAQIAEGLRYQLALPKEQATRRTLELLELVRVPDPSRVARSYPHEISGGMQQRA
                     LIAMALGVEPELIILDEPTTSLDVTTQAAVLDLLRSLIHDKNTAALYITHNLGVVAQL
                     CDRVAVLYASELVEDGETQALFQNPLHPYTKGLLDSVPRLGQNKSGYEL"
     assembly_gap    6706..6805
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7108..8142
                     /locus_tag="PLC06_04915"
     CDS             7108..8142
                     /locus_tag="PLC06_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89819.1"
                     /translation="MLDIQGLRVEYLVSRSITDAITNAILGRPTRTVKAVDEVNLSIR
                     PGHTLGLVGESGSGKTTLARTIVGLVERTDGVVTMTGLPLPSRLSERDINTLRQIQFI
                     FQNPEEALNPYLTIGETLQRPFITIGGKSRAEAREGARRMLEAVGLPVDYLGRLPGRL
                     SGGEKQRIAIARAFATNPDLLIVDEPVSSLDVSVQAAVLNLMERLQTENGSAMLFISH
                     DLAVVGYLADRIAVMYAGQIVEICSAETLSEFPHHPYTEALLHAVPGIAPQPLGQTIR
                     LQGDVPSQIDVPSGCRFHPRCPRFLGQVCVDVMPPHQQMEDGNWIACHIPPKELQQAQ
                     ERSYRGGGVE"
     gene            8143..9090
                     /locus_tag="PLC06_04920"
     CDS             8143..9090
                     /locus_tag="PLC06_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89820.1"
                     /translation="MVAYVLRRLAFLGLTIIITSLVIFIVTQMLPGDVARVVLGREAG
                     DEALAAFREAHGLNDPLPVQYTRWLANFVMGEWGTSLSTRSEIYPLVMERLKNSLLLT
                     GITMLIAAPLATVLGVLAGLHEDKPTDSAISIASLAVVGLPEFVTGLILIQIFSRWLG
                     WLPPNSLIESGATFAEKWPMLILPAMTATLVLLAYIIRLTRAGVIEELGRPYVRTATL
                     KGLPRHTVIMKHVLRNALLPTITVIAISFGWLISGLIVIENVFNYPGLGRLLTFAIDR
                     RDLPLIQAVAMVTVVGFGLANLIADLLYAWLNPRIRLSY"
     gene            complement(9196..>9530)
                     /locus_tag="PLC06_04925"
     CDS             complement(9196..>9530)
                     /locus_tag="PLC06_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRO89821.1"
                     /translation="STSARLSITSVHKVVKRAVNAENLHRSLSAHDFRHYRATQLLRA
                     GMPLEVVQEFLGHADVTTTRNIYAPVLGVQVVTEWLDNLDVTPKEALQDTSIIEHGDG
                     DVFKMLDL"
     assembly_gap    9531..9630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9631..10448)
                     /locus_tag="PLC06_04930"
     CDS             complement(<9631..10448)
                     /locus_tag="PLC06_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRO89822.1"
                     /translation="MNRKDLKLPISDRPIPDGLTWINEDAFLREAGAQSIKTETTYRS
                     GLRLFADWLQHYRKVGYSRNDTWPLGPERLTTDVILEYRNWLLANRSRATVTTYMAAI
                     VGYLNFLDGHDQLPEPVQLGKLQRQMARRQIERNQAENVIDLDIARQDIPKIIQYYDA
                     LPLPPENDQYNRRLTLLRDRAIVKMLYSTAARISEVVSLNRANVGNGRASYATIIGKG
                     NKGRTLHIRGYAREAVVAYLAERSDTNPALFVSHSRNSTSARLSITSVHKVVKRA"
     gene            10684..12885
                     /gene="glgB"
                     /locus_tag="PLC06_04935"
     CDS             10684..12885
                     /gene="glgB"
                     /locus_tag="PLC06_04935"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRO89823.1"
                     /translation="MNNSTLDQGAVDAIVGGYSGAPFELLGPHIIDGNLVIRSFQPDA
                     EAVNVVFSPDRKSGMTRVHDAGLFEVTLPEQSEILPYRLEVTYSGENVQLYEDPYSFP
                     PAMAELDIYLMGEGTHLHIYEQLGAHLVELNGVKGVHFAVWAPNARRVSVVGNFNNWD
                     GRRHPMRFHQAIGVWELFIPGLGEAEVYKYEIKTRYYNYTVTKADPVGFASEVRPNNA
                     SVVWDINKYQWNDSEWMAGRGQRHTVDKPISIYELHIGSWRRKNGTEWLTYRDLITDL
                     IPYVKDMGFTHIELLPVSEHPFDGSWGYQVTGFFAPSSRYGTPDDFMAFVDACHKAEI
                     GVFIDWVPAHFPTDEHGLGFFDGTHLYEHSDPRQGTQPDWGTYVFNYGRNEVTQFLTS
                     NAIFWLDKYHIDGLRVDAVASMLYLDFSRKPGEWVANRYGGRENLEAIAFIRRFNDEV
                     HKLYPTALTMAEESTSFPGVTRSTEEGGLGFDYKWNMGWMHDTLQYFKNDPIYRSFHH
                     GTMTFSLLYAFSEKFLLPFSHDEVVHLKKSMLDKMPGDVWQRFANLRLLYAYQWTHPG
                     KKLLFMGGEFGQWREWSEDRSLDWHLLNVDPKHGQIQSALRRLNQLYHEETALHEVEY
                     SWEGFQWIDLHDYERSILSYARLSPSNGEAILVVLNFTPIVRYGYRLGVPEPGTYQEL
                     FNSDAEAFGGSNVVNEPLPSKDSPWHGYKQSIELTLPPLAAVFLKRIPDEQ"
     gene            12875..>13491
                     /locus_tag="PLC06_04940"
     CDS             12875..>13491
                     /locus_tag="PLC06_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRO89824.1"
                     /translation="MSNNDRLYPLLFQPALKAYIWGGRNLETLLGRELPEGIVAESWE
                     IAAHEDGTSVVINGCYHGLSLKEVHAQLGLDLIGRNNAWAQERGRFPLLVKLLDASDM
                     LSVQVHPDDTYALAHEGNELGKSEMWVVLHADPGAEIILGVRKGVTPDQFHKGIVDGS
                     LDQLLHRVSVSAGDHVCVPSGTLHAILGGILIAEIQQNSNTTYRV"
BASE COUNT         2925 a         3508 c         3732 g         3126 t
ORIGIN      
        1 tggttgccgc ggctttgtgg ctcacaaact catggcgtcg cactcaactg gatattgccc
       61 ggataagaga cgagagagcc atgatcgagg gatacttcga caggttgacc gatctcttat
      121 taaacaagaa cctgcggcaa gcgaacccgg atgatgaggc ggctcgtgcg gctcgcgctc
      181 acactctgac tactctccgc aatcttgctc ccgaagatag ggggcaggtg gtgcgattcc
      241 tctatgaatc cgcgcttctc gacgcggccg agccgattgt tgatttgcag gcggccgatt
      301 tatcggggat gaccttatct caggtgcaaa tgcctgcggt gaacctgcgg aatgtacacc
      361 tagccggtgc tcaattgggc ggtgtggtca tgccgtcttc cgacttacgg gaaacaaatc
      421 tcggtgacgc cgatctggct aaagcagacc tcagtgagtc atatcttcgc ggcgcccacc
      481 tgcgtggagc cactcttgcc gaggccaatc tgcgtggcgc gattttgaca aaggcggtct
      541 tcgttgaggc gaatttgcgc ggtgcggatt tgtctgatgc aaacctctcg ggagccgact
      601 tgtctcgtgc cgatttaacc ggggctaatt tgaaaggagc caagctcaac aatgctatct
      661 tcgctgaagc gctcctggaa gatacggatg taagtttggc aaatttgaac agatcgaatt
      721 taagcggcgt ggttgggaat cgagccaact tcagtggtgc aaacctggcc ggcactcaac
      781 tcgttggtgc aaagcttaat ggcgctgtgc tagggggggc catcctgagc gaggctgact
      841 taaccgacgc cgaaatgcgc gaggcgaatc taaatctggc caatctccgt ggcgcgatcc
      901 tgactcaggc aatccttgcc ggcgctaacc ttcgcgatgc gattatgggg cgggtatcac
      961 tcagtggggc tgacctttcg ggcagtgatt taagtcgggc caatctgagc attgccaatc
     1021 taagccgggc taacttgaac cgtgcgaatc tggaagaggc gggtctgagc ggcgcagacc
     1081 tgcgcgcggc tcaattacgc ggtgctatcc tcagaggggc catcttgcgc ggggccattc
     1141 ttggcgacgc cgatttgagc cgcgcggatc tgacaaaagc gaacttgcgt tgggccaatt
     1201 tgaataatgc caatttgact ggggcagatt tgaccgaagt cgatctgact gatgccgaag
     1261 tttctccgca gactttggcc aaagctcgtt cagtgagcaa ggtcgatcgc cccgatacat
     1321 cacattttga gtccgcgccg gcagtcatcg cgcctggaca atccggccct ttgccggcat
     1381 cacgaactcc atcttaccag cggccgagcg accagccgga tgggaaacca aaacgttccc
     1441 tggaagaaaa atccaataaa tagaccgata tgaataacca gagatacggg ctagttccaa
     1501 tccagctcgc ttctggtcgc ccaatactgc tcggccggct caacgagact cgcgagcctt
     1561 tccttcagcg cgttcgtcca ggtgatatca agggcgtgta tattggagag tagatgctcc
     1621 gattgtgcgg cgccgctgag aacgatgtcc gcccaaggtt gcgccaatac aaaccccagg
     1681 gctaaagcgt cgatagttgt gtttaattcc gttgctactt gttttagtag cttcttcctg
     1741 gaagcaaaat cgggatcatc gttccttgcc gtcaaacggc cgttagccaa tgcctcttta
     1801 acgatcaccc ccatcccgga ttgatgcgct tcgtttaaag cctctgttgc ttcgcgggcg
     1861 agcagattcc aggtcgcttg cacggaaccg aataacggtt tgttcccaac cttgatagtc
     1921 atcgccttgc gaagcgtcct ggcctgattt gggccgctca gggagagacc cactaccatt
     1981 cccccgtctc tcatatcccc cagggcctca agtaccttcc agtcgtcgag caccccgcta
     2041 tcgagagttg ccgagtgaat ctgatagagg tccaaataat cacctaacaa acccaggctt
     2101 tcaccgattt gccttttgaa gaccgccaac gaatgatcct taacctcgtg cttttgagcc
     2161 gcgactcgcc agccggccgt gtaggtatag ccccacttcg atcccacaac cacctcggat
     2221 gactgtatgc ctcggctgcg aagccacgtt gacaagaagg attccgcctg gccataggag
     2281 cgagcagcat caaaatagcg gatgccgctt tcccatgcgg tatccagcac cgagtgcgcg
     2341 tgcgattcca tcgaagccat atcatagtcg tggcttaaat cggatgcatg acccaggttg
     2401 atgtaaccgg gcctgcccag cgcagccagg ccaagcccta tgggggtgac tttgaggccg
     2461 gtttgattga atgggatgag gttcattgat tatcctctct tgtcatagag gttcggtcga
     2521 acaagggccg atcaagagat ggcaacatcc tgctgttcca taaatgctat aaggcgttcc
     2581 tcgaaggggc catagccact ttggatgatc tccaacggca acttaagtct ggtcgagatc
     2641 tcttgagcct tttcctgcaa catcggatcc accgtttggg ctagatagat gagccgacga
     2701 tagttgcgga agtactcctc ttgtagttca ggaaatcgat ccaaccctaa cccgtgccac
     2761 accgttccat gaaaaccgcg caccagaaaa tcggtcagga aaaacgtccc cggctcttca
     2821 tccatcaggt cggcaaactt ctgccccccg tacatctcgt aacagtgcgg gccattgatg
     2881 cgctgcaagc catagatgtt tagcagttca tccagcgagc cctttgttcc gcactctccg
     2941 tatacgacga gaatgcggtc gtaatccata cggagagcca gatacttctg ctccaccgcc
     3001 gggccgatcc gctccggata catatgggcg attgcgggaa tgccgaacag atcgacctgc
     3061 cattgatgac gtttgataat agctgtcacc tcgcgagcca gtgcaccaca aataataaag
     3121 gccgttttgg gtgagtcgga ttgagcggga gtcatgattt gatcggctta ttgttgttcc
     3181 gtcataagcg tgggcagtgt aatggatcaa atagcattcg tcaaaaccgt cccttttggg
     3241 gaagcaggga gaccatgtcg aagcagtcaa aaattgatag aatcgtctgg gtttcagcta
     3301 gagaccctga taataaaggt gagtattaca ttgtaaactc gtcttcatag ccatcatcgg
     3361 aggtaataat gagtttgctt ggtacaacgt tccacgataa gcggtttggg ttggccgtga
     3421 ttatcagttt gctcctcggc ctcgctgcct gtgggggagg gggtcctgca caacaaatga
     3481 cgaatactcc ttcagggaat gaatcggccg aaattcaaac tggggccgag gaagcctcgg
     3541 agactcaatc aggccgcggc ggaaccctcc gcgttgccat gcagccggtg gtcaatattg
     3601 atccgatagc catatcctcg gatagcgaag ttctcgtggc caaccatgtc tatgattacc
     3661 tgattgacat tgaccatctt tccaaccccg tcccacgctt ggctacgggt tgggatatta
     3721 gtgacgatgg tttgacctat accctgccgc ttgctgaaga cgttacctgg catgacggca
     3781 gcgagtttag cgccgcggat gttgtatgga ccttcgagcg ccttcgtacg actgaaggtg
     3841 cgccaacgtt cgatctgtat agtaatattg cctcaattga ggcgacgggc gatttggagg
     3901 ttacgttcaa gttaacgaag cccaacccgt tcttcttgta cgatttgagt gataatcacg
     3961 ccctcatcct gaaagccggc acgaccgacg cggatactaa cttcaacggc accggtccct
     4021 tcgtggttac caattatcgg cctgaagagc gaatcgagat ggttgccaat gagaattact
     4081 ttattcccga acaacccaag ctttccggcc ttcaaataat cttcttcaac gatgatacgg
     4141 tggccgttga ggcgctgcgg ggcgggcaaa ttgacttcac gatgcgcatg tccgtggctt
     4201 tgtttgagag cctgaaagaa gagccgggaa tcatcactat ggatattccc accaacggat
     4261 tcgacttgat acgcctccgc tccgataggg cgccgggcaa cgatccgcgc gtcattcagg
     4321 ccctgaaatt ggcgacagat cgggaagcga tcttcaacgt tgttcagcaa gggtatggcg
     4381 ctctgggcaa tgacagcccc atcgggccgt tgtatgatgc ctatcatgat gagactgcca
     4441 ttcccccggc gcgggacgtt gaggccgcca gagccttgtt ggccgacgcc ggatatgggg
     4501 acggcctgaa gatcaccctg cacacacccg atacggggaa ccgccccgat ctggcggccg
     4561 tgttgaagga gcagtggtcc gaggttggcg tagatgttga gatcagcgtc gaaccggaga
     4621 gtgtgtatta cgccgatgat ggctggctgg aggttgatct ggggatcacc ggctgggggt
     4681 cgcgaccgta tccgcaattc tacctcgatg tgatgctggt ttccgacgct atttggaatg
     4741 agtcgcactt ctcggatgag gagtttgatt cactggcggc cgtcgccggc acgacccttg
     4801 atgaaaaaga gagaattcag gcatacgccg atatccagca cctgcttatt gagcgcgggc
     4861 cgattattat cccgtattat tttgcccagt tcggggccat aaacgatcag ttccagggat
     4921 tgaacctgaa ggcgtttgcc ggccggactg acttccgaac agttaccttt tcaggaaact
     4981 agccgatcaa gggcatggcc ggaagccggt catgcccttg atctcgccca ttcatcccca
     5041 aaaggactcc tcctaagcat gagagccatc aatgtgtcgt ccgtgttgcg cgagctatat
     5101 gcaaggcgga cgactgcgct gggaacgacg atcttcctcc tgtttctctt cgtcgcgatg
     5161 tttgctcccc tcttcgctcc ctatggtttg aatgagcaga tctacagcga cgcgcggcag
     5221 gctccgtcga ctgcccattg gtttggcaca gaccaattgg ggcgagacgt cttcagtcgt
     5281 gtgcttctgg gcgccaaaga tatcctcact ctagccggat tgggaacggc tctggccgtg
     5341 gctagtggga ccctgtttgg tctggccgcc ggctattggg gtggcacttt tgacgagatc
     5401 ctcatgcgaa tattcgacgg cctgctggcg atccctgcat tactgttggc gcttttgctg
     5461 ctgggaactt taggagcatc acgaaacagc atcctgatcg tcattgtgct gctctatacg
     5521 ccgattgttg cgcgggtggt gcgtagcgtt gttctgggcg tcaggagcaa gggatttgtt
     5581 gaggcggcgc gccttcaggg agaacccacg gggcgaatcc tgtttcgcga gattctgcct
     5641 tccgtcgtgc cgacactaac ggttgaagct gccctgcgct tttcatacgc gatcttcctc
     5701 gtggcgtccc tgggatttct cggcgtagga gttcagccgc caagccctaa ctggggtctg
     5761 atggttaacg aggcccgacc tcacgtgagc cagttgccct ggacaatgta ttatccggcc
     5821 ggcgcgatcg ctatcgtcgt gattggcgtc aacttgatgg ccgacgggat taaacgcatt
     5881 gtcaggacgt gattcgaaat acccgatgtt aaacattcaa ggtctttcag tcgcctacga
     5941 gcaaaatggc gttttgctgc ctgcggtgcg ggacttctcg ttatcgttag ccaaaggtca
     6001 aacctatggc ctggtgggcg agagtggttc gggcaagacg accgtagcca tggctattct
     6061 gggctatctg aggggagagg ggcgaatcac cggcggccga attgagtttg aaggccggaa
     6121 tttgctcacg ctgcctcaaa gcgaacgaat taaggttctg gggaaaagta tcgcgcttgt
     6181 cccgcaagat ccgatgtcat cattgaatcc atccatgcgc gttggggcac agatagccga
     6241 aggcctgcgc tatcagttgg cgttgccgaa agaacaggca acccggcgga ccctggaact
     6301 actggaattg gtacgggttc ctgacccgtc acgagtagcc cgcagttatc cccatgagat
     6361 tagcggtggc atgcaacagc gagctcttat tgccatggcg ctgggtgttg aacctgagtt
     6421 aatcattctt gatgagccaa ccacgagcct tgacgtgacc actcaggcgg ccgtcctcga
     6481 tttattacgc agtctgatcc acgataaaaa taccgctgcc ctttatatca cacataacct
     6541 gggtgttgtg gctcaacttt gcgatcgcgt cgccgtgctc tacgcaagtg agttggtcga
     6601 agatggcgaa acccaggcgc tctttcaaaa cccgctgcat ccatatacca aaggcttgtt
     6661 agatagcgtc ccgcgccttg gacaaaacaa atccggatat gaactnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnaaggc ttgttagata gcgtcccgcg ccttggacaa
     6841 aacaaatccg gatatgaact tcctgctatc ccagggagaa tccctgcctt aacggagcga
     6901 ccacccggtt gttgtgtctt tgctccgcgt tgcccggtgg cgatcgagcg ctgtttctcc
     6961 gagcgcccaa ttttggaatc ggcaatgggc gatgggcaga tacgatgtca tcgatggccc
     7021 gaaatcgcga gcggagagat aagccacgca gttaaggaga cattcgcccc cgttaaggcg
     7081 cgaacgtcga caatggggga gggggatgtg ctcgatattc agggtttgcg cgtcgaatat
     7141 ctagtttctc gctccatcac tgatgccatt actaatgcga ttttgggtcg gccgacgcgc
     7201 acagttaagg cggttgatga ggtgaatctg tccatccggc ctgggcatac tctcggtttg
     7261 gtaggggaga gcgggagcgg caagacgacg ctggccagaa cgattgttgg cctggttgaa
     7321 cgaacagatg gagtggtcac gatgaccggc ttgccgctgc cgtccagatt gtccgaacgc
     7381 gatatcaaca ccttgcgaca aatccagttt atctttcaga accccgaaga ggcgctcaat
     7441 ccatacttga ccatcggtga aacattgcag cggccgttta tcactattgg cggaaaatcg
     7501 cgtgcggaag ccagggaagg ggcacgacgg atgctggagg cggtcgggtt gcccgtcgat
     7561 tatttggggc gattgccggg tcggctcagc ggaggcgaga agcaacggat cgccattgct
     7621 cgagcatttg cgacgaaccc cgatttgctt atcgtcgatg agccggtatc ttccctcgac
     7681 gtatctgtcc aggcggccgt cctgaacctg atggagcggt tacagacgga gaacgggagc
     7741 gccatgctct tcatctccca cgatctggcc gttgttggct atctggctga ccgcattgcg
     7801 gtgatgtatg ccgggcagat tgtagagata tgcagtgcgg aaacgctgtc cgaatttccg
     7861 catcacccct acacggaggc gctgctccac gcggtgccgg gtatagcgcc tcaacccttg
     7921 ggacaaacga tcaggttaca gggagatgtg ccaagccaga tcgacgtccc gagcggctgt
     7981 cgatttcatc cccgttgccc gcgctttctt gggcaggttt gtgtggatgt gatgcctccc
     8041 catcaacaaa tggaagatgg taattggatc gcctgccata tcccccccaa ggagcttcag
     8101 caagcgcagg agcgaagtta tcgcggtgga ggagtcgagt aaatggtcgc ctatgtgctt
     8161 cgccgtctgg cattcctggg gctaacaatt attatcacat cactcgttat tttcatcgtg
     8221 acccagatgc tgccggggga tgtcgctcgt gttgtgttgg ggcgggaagc cggcgatgaa
     8281 gccctggcgg cgtttcgcga ggcacacgga ttaaatgatc ccttgccggt gcaatataca
     8341 cgctggttgg ccaatttcgt catgggtgag tgggggactt ctctgagcac tcgttccgag
     8401 atctacccgt tggtcatgga gcgactaaaa aactcgctgt tattgacagg tatcacaatg
     8461 ctgattgcgg ccccgttagc gacagttttg ggtgttttag ccggtttaca tgaagacaag
     8521 ccaacggata gcgcgatcag catcgcatcc ctggcggtcg tcgggttgcc ggagttcgtg
     8581 accggtctca ttttgatcca gatattctcc aggtggttgg ggtggcttcc tcccaactcg
     8641 ctcatcgaat cgggagcaac cttcgccgaa aagtggccca tgctcatctt gcccgcaatg
     8701 accgcaacgc tggttctgct ggcctatatc atccgcctga cccgagctgg agtgattgaa
     8761 gaattaggtc gcccctatgt tcggaccgcc acactaaaag gattgccccg ccataccgtg
     8821 atcatgaagc atgtgctgcg taacgcactg ttgcccacca ttaccgtcat cgccataagc
     8881 ttcggctggt tgattagcgg acttatcgtg attgaaaacg tgttcaacta ccctggcctg
     8941 gggcgcctgc taacctttgc aatcgaccgc cgcgatctgc cactcataca ggctgtagcg
     9001 atggtcacag ttgtagggtt cggtctggcg aatttaatcg ccgatctgct ttatgcgtgg
     9061 ctaaacccgc gaatccgttt aagttattga gctgcaatta ctggagcatg ggagggctgc
     9121 agcatatagc acaggaaaat attcgtgtag ccatagtaca cacccataaa tccagcctac
     9181 gggcgaggac agggactaaa ggtccagcat tttaaagaca tctccatccc cgtgctctat
     9241 gatagatgta tcttgcagcg cttctttagg tgtaacatcc agattatcca gccactcggt
     9301 gacgacctgg acgccaagaa caggggcata gatattacgt gtggtcgtta cgtcggcatg
     9361 gcccaggaat tcctgtacga cctccagtgg catgccggca cgcagcaatt gtgtggcccg
     9421 gtagtgcctg aagtcatggg ccgacaggct ccggtgcaga ttttctgcgt tcactgcgcg
     9481 cttcacgacc ttgtgaacag acgtgatgct caacctggcg ctggtggagt nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcgcttca cgaccttgtg aacagacgtg
     9661 atgctcaacc tggcgctggt ggagttgcgg ctgtgggata caaagagggc agggttggtg
     9721 tccgaccgtt ctgccagata agccacaacc gcttctcggg catagccacg aatgtgaagg
     9781 gttcgaccct tgttgccttt gccgataata gttgcgtatg atgcgcggcc gttgccgaca
     9841 ttggcccggt tcagcgagac gacctccgag attcgcgccg ctgtggaata gagcatcttg
     9901 acgatcgccc ggtcgcgcag caaggttaat cgccgattat actggtcatt ttcagggggc
     9961 aggggcagtg catcgtagta ctggattatt ttggggatat cctgccgcgc aatgtcgagg
    10021 tcgatgacat tttctgcctg attgcgttca atctgccgcc gggccatctg acgttgcagc
    10081 ttgccgagtt gaacaggctc aggcagctgg tcatgaccat cgaggaagtt caggtatccg
    10141 acgatggcgg ccatataggt cgttacggtt gcccgggagc gattggctag cagccagttg
    10201 cggtattcca aaatcacatc ggtggtgagt cgttcggggc caagaggcca ggtgtcgttc
    10261 ctggagtagc ccaccttgcg gtaatgctga agccagtcgg caaacaggcg caacccgcta
    10321 cggtaggtgg tttcggtctt tatgctctgc gcgccggctt cgcgcaggaa tgcatcttca
    10381 ttaatccatg tcaatccatc gggtatcggg cgatcagata tgggcaactt gaggtctttt
    10441 ctgttcatgg ttttggggtg gtgggccaac aggttttcag atcacacgca gtgtgatgtg
    10501 cgataatcca tagttaaatt aacatacatt attaatatca tgaattatag agagtgattg
    10561 aatcatgtca acccccataa cgtgcagttc gactgaatca ggctaaaatg tgtacgtggc
    10621 tgttaaggct cgttaaagtt gccggcggag agcaagaggt gagcctgcaa agcgaggaca
    10681 tgtatgaaca attcgacact ggatcaggga gctgtggatg caattgtcgg tggctactcc
    10741 ggcgcaccgt ttgaactgct tgggccccat atcatagacg gcaatctggt gattaggagt
    10801 tttcagcccg acgctgaagc cgtcaatgta gtgttttcac cagatcgaaa atccggcatg
    10861 actcgggtac atgatgcggg gctatttgag gttaccttgc ctgagcaaag cgagatcctg
    10921 ccatatcgcc tggaagtgac ttattcgggc gaaaatgttc agctatacga agacccctac
    10981 tcatttccac cagccatggc cgaattggac atctatttga tgggcgaagg gacgcatctc
    11041 catatctacg agcagttggg tgcccatctc gttgagctga acggggtcaa aggcgtgcac
    11101 ttcgccgtgt gggcgccaaa tgcccggcgg gtcagcgtcg tgggcaattt taataattgg
    11161 gacggccgcc gtcacccgat gcgtttccac caggctatcg gcgtttggga actgttcatc
    11221 cccgggctgg gcgaagcaga ggtctataaa tacgagataa agacccgcta ctacaattac
    11281 acggtgacaa aggcggatcc cgtcggcttc gcctcagaag tccgtcccaa taacgcctca
    11341 gtggtttggg acatcaacaa gtatcaatgg aatgacagcg aatggatggc cgggcgcggg
    11401 cagagacaca ccgtcgataa accgatcagc atctatgagc ttcatatcgg ctcgtggcgc
    11461 cgcaagaacg gcacagagtg gttgacctat cgcgacctga ttaccgacct gatcccgtat
    11521 gtcaaagata tgggcttcac ccacatcgaa ctactccctg tctccgagca ccctttcgac
    11581 ggctcgtggg gatatcaggt gacggggttc ttcgccccca gcagccgtta cggcacgccg
    11641 gatgatttca tggcgttcgt cgatgcgtgc cataaggcgg agatcggcgt gttcatcgac
    11701 tgggtgccgg cccacttccc caccgatgag catggcctcg gctttttcga cggtacacat
    11761 ctatacgaac attccgatcc tcgtcagggg acgcagcccg actggggcac ctacgttttc
    11821 aactatggcc gaaatgaagt gacgcagttc ctgaccagca acgcgatctt ctggctcgat
    11881 aaatatcaca tcgatggtct gagagtggac gcggttgctt cgatgctcta tctggacttt
    11941 tcacgcaagc cgggcgagtg ggttgccaat cgctatggcg gccgcgaaaa cctggaggcc
    12001 attgccttca tccgtcgctt caacgatgaa gtgcacaagc tctatccgac tgccctcacc
    12061 atggccgaag aatccacctc ttttccgggc gtgactcgat caacggaaga aggtggcctg
    12121 ggcttcgact acaaatggaa tatggggtgg atgcacgaca ctctgcaata tttcaaaaac
    12181 gacccaatat atcgctcctt tcatcatgga acgatgacct tttcattgct gtatgccttc
    12241 agcgagaaat tcctgctgcc gttttcccac gatgaggtgg ttcatctcaa aaagagcatg
    12301 ctggacaaga tgcccgggga cgtgtggcag cggttcgcca acctgcgctt gctttacgcc
    12361 taccaatgga cgcatcccgg caagaaactg ctgttcatgg gtggcgagtt tggccaatgg
    12421 cgtgaatgga gcgaagatcg aagcctcgat tggcatctgc tgaatgttga tccgaaacat
    12481 ggccagatcc aatccgcgtt acgtcgactc aaccagctct atcatgaaga gaccgccttg
    12541 catgaggtgg agtattcctg ggaaggattc caatggattg atctccatga ttatgaaaga
    12601 agcattctaa gctatgccag gctctcaccc agcaacggag aagcgatcct ggtagttttg
    12661 aacttcactc caattgtccg ttatggatat cgactcggtg ttcccgaacc aggtacctat
    12721 caagaacttt tcaatagcga tgcagaggca ttcggcggca gcaatgtcgt caatgagcct
    12781 ctaccgagca aagactcgcc ctggcatggt tacaagcaat cgattgagct tactctccct
    12841 cccctggcgg ccgtttttct gaagcgcatc cccgatgagc aataacgaca ggctttaccc
    12901 gctgctattt caacctgccc tgaaagccta tatctggggc gggcgaaatc ttgagacact
    12961 cctcgggcgc gaattacccg aagggatagt agccgagagc tgggagatcg ccgcccatga
    13021 ggacggcact tctgttgtca tcaatggctg ctatcatgga ctatcattga aagaagtcca
    13081 tgctcagctg gggctagatc tcattggccg taacaacgca tgggcacagg agcgaggaag
    13141 atttcccttg ctggtgaagc tcctggatgc gtcggatatg ctctcggttc aggttcatcc
    13201 tgatgatacc tacgcattag cccatgaggg aaatgagctg ggaaagagcg aaatgtgggt
    13261 tgtcctccat gccgacccgg gcgcggagat tatccttggc gttcggaaag gcgttactcc
    13321 cgatcagttc cacaagggga ttgttgacgg atctcttgac caactcctcc acagggtttc
    13381 agtaagcgca ggtgatcacg tgtgtgttcc cagtggaacg ctgcatgcga tattgggtgg
    13441 cattctgatc gccgagattc agcagaactc caacacgacg tatcgtgtat a
//
LOCUS       DAOMQG010000054        13384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1989_length_13384_cov_10.376397, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000054 DAOMQG010000000
VERSION     DAOMQG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13384
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_13384_cov_10.376397"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PLC06_04945"
     CDS             <1..1512
                     /locus_tag="PLC06_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRO89825.1"
                     /translation="LSAAIVGLDAELVDVEVDIVKGAPYFALVGLPDAAVRESRDRVY
                     AAVKNSGFTFPLHRITVNLAPADLRKEGPAYDLPIAVGVLAATSQIWHNLLDGSLFFG
                     ELSLDGATRHTKGVLPLAAMARERGLKRLFVPADDAAEAALMPDLEVFPVRTLAELVG
                     HLTGMEPLEPLRVDLEAAFDHEVEYATDFADIMGQEHVKRAMEVSAAGAHNALMTGPP
                     GSGKTLIARALPGILPRMGIDEALEVTKIYSVAGMLPPETPLVRARPFRSPHHTISYA
                     GLVGGGQWPRPGEISLAHRGVLFLDELPEFGERLIEVLRQPLEGMPREVSISRATGSV
                     TYPANFMLIAAQNPCPCGYYGDPTHGCTCSSAMVTRYQKRISGPLMDRIDLHVDVPRV
                     DYEKLSSTQRGELSAGVRARVEAARERQAQRFAGTGLYANADMGPGEVRQYCTLDETG
                     RRLMKSAMTQMGLSARGFHRVLKVGRTIADLAGAADIGPAHLAEALQYRARTT"
     gene            complement(1581..2336)
                     /locus_tag="PLC06_04950"
     CDS             complement(1581..2336)
                     /locus_tag="PLC06_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO89826.1"
                     /translation="MNESRQAADAFDIAARHWLAKRGKKKVKFVLERDDGAGIEDFID
                     NYVYKGSSSLFGLERRLLNYIKSPVLDLGCGAGRVAIPLCRAGHSVMGIDISGSILRI
                     GRDNSKDCHIPFIQMSMNQLGFAPDTFSTIICMHNTIGVTENLGGLKNLLHQLRRPCM
                     KGGYLVLTSIDVTRYPDYETYFLKNFQKGRAMGQVRMRIRFGKIMGDWFDWLYVLPDD
                     LARIASETGWKAADIFYDVNDSRTYAAVLQNGR"
     gene            2561..2824
                     /locus_tag="PLC06_04955"
     CDS             2561..2824
                     /locus_tag="PLC06_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRO89827.1"
                     /translation="MADLFPKARHNIEAKEIGDETLLISGDDIHVLNETAAFIWKLCD
                     GTCDPAAIEAALREEYDVPSDRDVAIDVARALADFRSKGLLDA"
     gene            2859..3866
                     /locus_tag="PLC06_04960"
     CDS             2859..3866
                     /locus_tag="PLC06_04960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89828.1"
                     /translation="MVSIITPTYNRPDLLREAIGSVLNQSFDDWEMLIVDDGSEPGAH
                     PVVDGFADPRLRYVRLGHVGRSAARNRGLELARGMYIGFLDDDDLYHPDKLAREISFL
                     CAHPGVDIVGSGYRAVGKMGEVLKTYENWTVKPEINRENCLFGVPLATCSVLIARKAI
                     QSMDRWFDPALDLWEDSDFFRRLFLTGIRFAWLREVLSDYRLIHDRSWSVILDVQRTG
                     RRALGKLFETEDLPPELAGQRQDALVKFDLDYAWMAYLYGAENLGQRSMLQALVREPR
                     LADQRADVVLGELATYSQRIEDPDSYIDYVLSHLPSPLCHLADRGEEVKALVVSRAGL
                     A"
     gene            4352..5341
                     /locus_tag="PLC06_04965"
     CDS             4352..5341
                     /locus_tag="PLC06_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89829.1"
                     /translation="MFSNFDNIFGVPALVMGDEVHPLEEPIPLLKFHSYVMHEIQQRV
                     DTHFLVHAGVVSRHEKGILLVGDANFGKSTLVLTLLKRGFTFLSDELAAISRVDGKVY
                     PYPRYLGIRPETLKHLGLKIPNTTNDDFDSYPFNIGKHLINVESLFPGRLGSPVPIRA
                     ICILSDPKIMPEAPGTSTLRLIHLWVGPYPAAFLSELTRLNGVSNLVTNNESDPALIT
                     LTATDKVGTMDQARSLCARHGVTLVGISDRMGHKPAFDVPPRLSAISHSQATRYLFQR
                     FLGGREALLRATPAGRPAQLFAELMELIGGAACYTLSVGPLTRMTDLIDEIAG"
     gene            5343..5930
                     /locus_tag="PLC06_04970"
     CDS             5343..5930
                     /locus_tag="PLC06_04970"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRO89830.1"
                     /translation="MPTDSKPPTPPAVLDAALQSWGNAGRQIVFPMSGQSMEPFLKAG
                     DQLLITHGREHIRVGTLVAYRQDDKLIVHRLLHVFDRSREPMMLLRGDNNNYPDLLIP
                     LDRLIGRVSAVRRGDRSYTLETPLWRGASRLIAACYRWPITLGQWAGAARRRLFPNRP
                     VSDFGRSYRLSLRVFTIPARFLTFMLWKFARYDRI"
     gene            5941..6105
                     /locus_tag="PLC06_04975"
                     /pseudo
     CDS             5941..6105
                     /locus_tag="PLC06_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
     gene            6210..7274
                     /locus_tag="PLC06_04980"
     CDS             6210..7274
                     /locus_tag="PLC06_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRO89831.1"
                     /translation="MDQTEQFLKALTEAHGVPGYETEVRAVLRQYMSPLGDLSQDKLG
                     SLICRQPGDGPRVMLAGHMDEIGLIITHIDDSGFLKFLQLGGWWDQTLLSQRVIVKTH
                     KGDIVGVVGAKPPHLLSADERGKMVEKKDMYIDIGSTSKAETEAAGVRVGDPVVPDSE
                     FVVMAGGKTYLAKAFDNRVGCAVVIDVLRHYRNAAHPNDLYGVQTVMEEVGVRGATTS
                     ARAVDPDVAIVLESDIAGDVPGIKNEQSSVRLGGGPGLSVFDSRMIPNTKLRNLVIDT
                     AAEQGIPLQFSNMPGGATDGAAIHMNNVGVPTVVIGVPSRHIHSHGSIIHRDDYDNAV
                     KLVTALVAKLDAATVAELTL"
     gene            7437..9110
                     /gene="atpA"
                     /locus_tag="PLC06_04985"
     CDS             7437..9110
                     /gene="atpA"
                     /locus_tag="PLC06_04985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRO89832.1"
                     /translation="MATLAPDFQRIADSLLDQIKAFDHKVTPRSVGTVSAVYDGVALA
                     TGLADVAANELVEFSNGVAGIALDLQPDNVGIVVMGDYTGIEVGDEVRATGNIASVPV
                     GEAMIGRVVDPLGNPIDGKGPINTTKRRPLQRTAPGVIERKGVDTPVQTGILSIDSMF
                     AIGRGQRELIIGDRQTGKTAIALDTIISQKGQNMICIYVAIGQRAGQVAQVVATLEKY
                     GAMDYTTVVMAGAADPAPLQYFAPYSGCAIGEEFMENGKDALVVYDDLSKHAWAYRQM
                     SLILRRPPGREAYPGDIFSLHSTLLERAVRLRDEYVIVEKGANVTAKTKGVDGKVYFG
                     NLVDEELHKAFEALGEGAKDKYEARKIPGTGGSLTALPIIETLLGDVSAYIPTNVISI
                     TDGQLFLETDLFNAGQRPAISAGLSVSRVGSAAQTRAMSKASGTLKGDLSQFRELAAF
                     ASFGSDLDASTQRQLARGERLMELLKQPQYQPIRLDHEVFMIYAGTRGYLDNVAVKEV
                     QRWKAEMSRFMDTTYPEVGRNILETGGWNNDIEAAVKQGIIDFNNTWSN"
     gene            9198..10106
                     /gene="atpG"
                     /locus_tag="PLC06_04990"
     CDS             9198..10106
                     /gene="atpG"
                     /locus_tag="PLC06_04990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRO89833.1"
                     /translation="MATERELNKRIKSVKNISQVTSALAAVSASKASRAQRQAEATRH
                     YAQKAFEIIQNLAAQPGASATTHPLMLARSQVRNIGLILMVSDRGLAGSYNVNMITLA
                     DRFIAAAGKPVKIITVGRKGRDAMVRRGRDVVATFDRIPDPPAFLDITAVARVAIDSM
                     LSEDVDQVFIGYSEFVNMVTRQPVIRQLLPLRPAHPDEIPGAEYVSGVDAAKASQQSY
                     TYEPSAEALLHDIVPRFTRLQVYQSLLEAFASEHSARMVAMNNATDNAAELVDILTLE
                     RNKARQASITNEILDIVGGAEALKNM"
     gene            10243..11637
                     /gene="atpD"
                     /locus_tag="PLC06_04995"
     CDS             10243..11637
                     /gene="atpD"
                     /locus_tag="PLC06_04995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRO89834.1"
                     /translation="MATGKITAIRGVVVDVEFPEGELPEIYEALHVEAPQGQLVLEVQ
                     QHLGGGDVRTVAMGSTDGVPRGRDVVATGGPIMVPVGPVTLGRIFGVTGEAVDGRPTP
                     KADTYYPIHRAAPEFQDQSTAIETFETGLKVVDLLAPFIKGGKTGIYGGAGVGKTVTI
                     AELIRTVAQEHEGVSVFAGVGERTREGTDLYYEMQEYGVQDSVAMVFGQMNETPGVRL
                     RVALTGLTMAEYFRDEGRDVLLFIDNIFRYVLAGSEMSALLGRMPSAVGYQPTLAAEM
                     GALQERITSTRKGSITSMQAIYVPADDYSDPAPVATFAHLDAIITLERSVFARGIFPA
                     VDPLASSSRALQPDVVGELHYRTAREVKQVLQTYKDLQDIIAILGIDELSEDQKLLVA
                     RARKIERFLGQPMFVAEKFHGLNGQYVPTAETVRGFREILDGKHDDLPEQAFYMVGGM
                     DQVLEKAEQLRAMA"
     gene            11728..12192
                     /locus_tag="PLC06_05000"
     CDS             11728..12192
                     /locus_tag="PLC06_05000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRO89835.1"
                     /translation="MPIQCDIITQERIVFSGQVDSVNLPGAEGRMGVLPNHSPLLTTL
                     DFGEVYIRRSGAEEYFAIGGGFVEIQPDHVTILADSAEQANEIDIERAERARQAAEEI
                     MRTGVKDDPDRIAAVRASLLRAQARVDVGRRRRRHHAVPMGGGHHPDDQDKN"
     gene            12387..>13384
                     /locus_tag="PLC06_05005"
     CDS             12387..>13384
                     /locus_tag="PLC06_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRO89836.1"
                     /translation="MSLFTPQIAIDLGTVNVLVHVQGRGVVLQEPSVVAIREDGNRTI
                     IVEVGRAAKEMYGRTPGEIEVMRPLRDGVIADYFVTQGMLEYFITKVAGRIPLRKPSV
                     MISVPYGVTSVERRAVREAALAAGAREVNLIPEPLAASIGAGLPVGTPTGNMVVDLGG
                     GSTEAAVVAMNGIVAAESVRVGGVHLDEAIISYVRKKYNLVIGEPTAEAIKIKIGAAM
                     ELDERLEMQVQGRDQVAGLPRTITMTSSEVTEAIVEQLQAIINVTRAVLAKTPPELSS
                     DIIDRGMALTGGGALLRELDTLLTKETGVPAYVADNPIACTALGAGKALAELPVLQR"
BASE COUNT         2662 a         4266 c         3911 g         2545 t
ORIGIN      
        1 ctcagtgcgg ccatcgtcgg tctggacgcc gaactcgtcg acgtcgaagt cgacatcgtc
       61 aagggtgcgc cctatttcgc cctggtcggt ttgcccgacg cggccgtgcg cgagagccgc
      121 gaccgggtct atgccgcggt gaagaacagc ggcttcacct ttcccctcca tcgcatcacc
      181 gtcaacttgg ccccggccga tctgcgcaag gaaggcccgg cctacgacct gcccattgcc
      241 gtgggcgtgc tggccgccac gtcgcagatc tggcacaacc tgctcgacgg ctcgctgttc
      301 ttcggcgaac tgtcgctcga cggcgcgacg cgccacacca agggggtgct gccgctggcg
      361 gccatggccc gcgagcgcgg cctcaagcgc ctgttcgtgc ccgccgacga cgcggccgag
      421 gcggctctca tgcccgatct ggaggtgttc cccgtgcgca cgctggccga actcgtaggc
      481 catctgaccg gcatggagcc gctggagccg ctgcgcgtcg atctggaagc cgccttcgat
      541 cacgaggtgg aatacgccac cgacttcgcc gacatcatgg gccaggagca cgtgaagcgg
      601 gcgatggaag tgagcgccgc cggagcgcac aacgcgctga tgaccgggcc gccgggcagt
      661 ggcaaaacgc tcattgcgcg cgccctgccg ggtattttgc cgcgcatggg catcgacgag
      721 gcgctggaag tgacgaagat ctacagcgtg gccgggatgc tgccgccgga aacgccgctg
      781 gtgcgggcgc ggccgtttcg ctcgccccat cacaccatca gctacgccgg gctggtgggc
      841 ggcgggcagt ggccgcggcc gggcgagatt tcgctggccc atcgcggcgt gctgttcctc
      901 gacgagctgc cggagttcgg cgagcggctg atcgaggtgt tacgccagcc gctggaaggc
      961 atgccgcgcg aggtgtcgat tagccgggcc acgggcagcg tcacctatcc ggccaacttc
     1021 atgctcatcg ccgcccagaa cccctgcccc tgcggctact acggcgaccc gacccatggc
     1081 tgcacgtgca gcagcgccat ggtcacccgc taccagaagc gaatcagcgg gccgttgatg
     1141 gaccgcatcg acctgcatgt ggacgtgccg cgagtggatt atgagaagct gtcctcgacc
     1201 caacgcgggg agctgtcggc cggggtgcgg gcgcgggtgg aagcggcccg cgagcggcag
     1261 gcgcagcgct tcgccggcac gggcctctac gccaacgccg acatggggcc gggcgaggtg
     1321 cggcaatact gcacccttga cgagaccggc cgccgcctga tgaagagcgc catgacgcag
     1381 atgggcctca gcgcgcgcgg cttccatcgc gtgctaaagg tcggtcgtac cattgccgac
     1441 ctggccggcg cggccgatat cggtccggcg cacctggccg aggcgttgca gtatcgagcg
     1501 cggacgacgt agcgtcgcgc ctcaaattgt tacgccacac tatagcaagg ccacctggac
     1561 aatcggatgc ggctatgatg ttagcgcccg ttctgtaata cagccgcata agtccgcgag
     1621 tcattaacat cgtaaaagat gtccgcggct ttccaaccgg tttcagaggc gatgcgcgcg
     1681 agatcatcag gcaatacata tagccaatca aaccagtccc ccatgatctt cccgaacctg
     1741 attcgcattc tgacctgacc catagctcga cctttttgga aattctttaa gaaataggtc
     1801 tcataatcag ggtacctggt aacgtcgatc gaggtaagaa caaggtatcc gcctttcata
     1861 catggccggc ggagttgatg cagcaggttt tttagtcctc caaggttttc ggtgacgcca
     1921 atagtattgt gcatgcagat aatagtagaa aatgtgtcgg gagcgaaccc caattgattc
     1981 atcgacatct gtataaatgg gatatgacaa tcctttgaat tgtctctgcc tatcctcagt
     2041 atgctgccgg aaatatctat gcccattacc gagtgacccg cgcgacacaa ggggatggca
     2101 actcttcccg cgccacaacc taaatccaga accgggcttt tgatatagtt aagcaatctc
     2161 ctttcaaggc caaacaggct ggaagacccc ttgtagacat aattatcaat aaagtcctct
     2221 atgccggcgc catcatctct ttccaaaacg aatttaacct tcttcttgcc gcgcttcgcc
     2281 aaccaatggc gcgcggcgat atcaaaggcg tcagcagctt gccggctttc gttcattttg
     2341 ctctctcctt gcttcattgc cccttagagt gtcatgggat accatcaatc aggagcaata
     2401 gccagtgcat tctcgaatca atttcattgg gccggtttgg attattccag cagccgtaac
     2461 tctttccctg ctcggctcgg tggttgacgt tggagtcatg gcgccttgag gcgatagcct
     2521 gattgaatga attataagct gaaataacag ggaggataat atggccgatc ttttccccaa
     2581 ggcccggcac aatattgaag caaaagaaat cggcgacgag acgttgctca tcagcggtga
     2641 tgatatccat gtactcaacg agacggccgc gttcatctgg aagctgtgtg acggcacgtg
     2701 cgacccggcg gctatcgagg cggcgctgcg cgaggaatat gacgtgccgt cggatcggga
     2761 tgtggccatc gatgtggcgc gcgctctggc cgatttccgg agcaagggcc tgctcgatgc
     2821 ctgatgcagc gttgctcgcc ggcaatagtc ccgtgcctat ggtcagcatt atcacgccga
     2881 cctacaaccg cccggatttg ctgcgtgagg ccatcggctc cgttctgaac cagtcgttcg
     2941 atgactggga gatgctgatc gtggacgacg gctccgaacc gggcgcccac cccgtcgtcg
     3001 acggtttcgc cgacccccgc ctgcgctatg tccggctggg ccacgtcggc cgcagcgccg
     3061 cccgcaaccg cggcctggag ctggcccgtg ggatgtacat cggcttcctg gatgacgacg
     3121 acctctacca tcccgacaag ctggcccgtg agatatcttt cctgtgcgct catcccgggg
     3181 tcgatatcgt cggctccggc taccgggcag tgggcaagat gggcgaagtg ttaaagactt
     3241 atgaaaattg gactgtgaag ccggagataa acagggagaa ttgcctgttt ggtgtcccgc
     3301 tggcgacgtg ttcagtcttg atagcgcgaa aggctatcca atccatggat cgttggttcg
     3361 acccggcact ggacctgtgg gaggattccg attttttcag gcgtttgttt ctgactggca
     3421 tccgcttcgc ctggctcaga gaggtgttga gtgattatcg cctgattcac gatcgctcct
     3481 ggtctgttat tctggacgtg caacgcaccg gtcggcgggc gctggggaag ctctttgaaa
     3541 cggaggatct gccgccggag cttgccggcc aacgccagga tgctttggtt aagtttgatc
     3601 tggattatgc ctggatggcc tacttatatg gggcggaaaa tttaggtcaa cgttccatgt
     3661 tacaggccct ggttcgtgaa ccgcgcctgg ccgaccaacg ggcggacgta gtgttgggag
     3721 agctggcaac ttactcgcaa cgcatcgaag accccgattc atacatcgac tacgtcctga
     3781 gccatctccc ttccccgctg tgccatctgg ccgaccgagg tgaggaggtc aaggcgctgg
     3841 ttgtttccag agccggatta gcgtgatgac ccatttgccc gtcaacgccg aagggtacat
     3901 aatatctggc aggatacttg atagtccaca tcgttccaga caaaaagctg cccggcaaaa
     3961 tcaatgtggg agaaaaatca tgatgaagga caagaaagaa tacgtggaac cgcaggtgac
     4021 cagctacagt gacgaggaaa tcctggctga gttgggtgag gtacatgcct ttccgccgcc
     4081 gatcagcccg taattaaagc ggttggcctc taattttcgg cggcgaatct gtccgtggcg
     4141 ggagatctga ttagtccgca gtggggcagg ttgtcttttc atatttcagg aggagggttt
     4201 gacgcacacg gcagagatca ctctctcctt ctttaatacg cgtgtggcga ttcggagcga
     4261 tgatcctcgt catctcgatc tgtttgctcg cctctatccc cggtttgttt ccgcaagcac
     4321 aaacgagccg gcaaacgctt tgcgctttag cgtgttcagc aatttcgata atattttcgg
     4381 cgttcctgcg ctggtcatgg gggatgaagt tcatccactt gaagaaccga tccccctgct
     4441 taaattccac tcttatgtca tgcatgaaat tcagcaacgg gtggatacgc attttctggt
     4501 ccatgccggc gtagtcagcc gccatgaaaa aggtatcctg ctggttggag atgcgaattt
     4561 tggcaaatcc acactggtat tgacattgtt aaagcgggga ttcaccttcc tctcggatga
     4621 acttgccgca atcagtcgcg tcgatgggaa ggtctatccc tacccacgat atctggggat
     4681 tcggcctgag accttgaagc atctgggact caaaattccg aatacgacca atgatgattt
     4741 cgacagttat ccttttaata ttgggaaaca tttgattaac gttgaaagcc ttttccctgg
     4801 ccggctgggt agccccgtcc ccatcagggc catctgcatc ctgagtgatc caaagatcat
     4861 gcctgaggcg ccaggcactt caacccttcg tctgatccat ctgtgggttg gcccatatcc
     4921 ggcggcattt ctctctgaat taacacgact caacggtgtg tcgaaccttg tgaccaacaa
     4981 cgagagcgat cccgccttga ttaccctgac cgcaaccgac aaggtcggga ccatggacca
     5041 ggcgcgatcc ctttgcgccc gacatggcgt tacccttgtg ggcatctccg atcgcatggg
     5101 tcataagccc gcgttcgacg tgccgccacg attgagtgcg atcagccaca gtcaggcaac
     5161 ccgctacctc ttccagcgct tcctgggcgg ccgcgaggcg ctgctgcggg ccacgccggc
     5221 cggccggccg gcgcagttgt tcgccgagct gatggagcta attggcggcg cggcctgcta
     5281 tacgctcagt gttggccctc tcacccggat gactgatctg atcgacgaga ttgccgggta
     5341 atatgcccac ggacagcaag ccgccgaccc cgcccgcggt tctggatgcc gccttgcaaa
     5401 gttggggcaa cgccgggcgg caaatcgtct tccctatgtc cgggcaaagc atggagcctt
     5461 ttctaaaggc aggcgatcag ttgctgatta cccacggtcg ggagcatatc cgtgtcggca
     5521 cccttgttgc ctatcgccag gacgacaagc tgatcgtcca ccggttactg cacgtgttcg
     5581 atcggagcag ggagccaatg atgttattgc gcggtgataa taataactat cccgacctgc
     5641 tcatcccgtt ggatcggctc atcggccgcg tcagcgctgt tcgtcgcggc gaccggagct
     5701 atacgctcga gacgccgctt tggcgcggag cctcgcgcct catcgccgcc tgctatcgtt
     5761 ggcccatcac tcttgggcag tgggccgggg cggcgcgacg gagattattc cccaatcgcc
     5821 ctgtatcaga tttcggcaga tcgtatcggc tctcattgag ggtcttcacc atccccgccc
     5881 gatttctgac ctttatgcta tggaagtttg cgcggtacga tcgaatatag taacgacacc
     5941 ggccgccgcc tgatgaaaag cgccatgacg caaatgggcc tcagcgcgcg cggttttcat
     6001 cgcgtgctga aggtgggccg caccatcgcc gacctggccg gcgcggccga tatcggcccg
     6061 gcgcatctgg ccgaggcgct gcagtatcga gcgcggacga cgtagcgtca tcggcgtaac
     6121 gcagttatcc ctctcaattg gacaattccc cgcacttaag ctaggatcac catcctgcgg
     6181 acaccctata tataggaata acgaaccacg tggatcaaac cgaacaattt ttgaaagcgt
     6241 tgacggaggc ccacggcgtg cccggctatg aaaccgaggt gcgcgccgtc ctccgccaat
     6301 acatgtcccc tcttggcgac ttgtcgcagg acaagctcgg cagcctcatc tgtcgccaac
     6361 ccggagacgg cccccgcgtc atgctcgccg gccacatgga cgagatcggc ctcatcatca
     6421 cccatatcga cgacagcggc tttctcaagt tccttcaact cggcggttgg tgggatcaga
     6481 cgcttctctc ccagcgcgtc atcgtcaaga cccacaaggg ggacatcgtc ggcgtcgtcg
     6541 gggccaagcc gccccacctg ctctcggccg acgagcgcgg caagatggtc gagaagaagg
     6601 acatgtacat cgacatcggc tccaccagca aagccgaaac ggaggccgcc ggcgttcgcg
     6661 tgggcgaccc cgtggtgccc gacagcgagt ttgtcgttat ggccggcggc aagacctacc
     6721 tggccaaggc gttcgacaac cgcgtcggct gcgccgtcgt catcgacgtc ctgcgccact
     6781 accggaacgc ggcccatccc aacgatctct acggcgtgca gaccgtcatg gaggaagtcg
     6841 gcgtacgcgg cgccaccacc agcgcccgcg ccgtggatcc cgatgtggcc attgtcctgg
     6901 aatccgacat cgccggcgac gtgcccggca tcaagaatga gcaatcgagc gtgcggctgg
     6961 gcggcggccc cggcctgtcg gtcttcgatt cgcgcatgat ccccaatacg aaactgcgca
     7021 atctggtcat cgacacggcc gccgagcagg gcattcccct gcaattttcc aacatgcccg
     7081 gcggcgccac cgacggcgcg gctattcaca tgaacaacgt cggcgtgccc acggttgtca
     7141 tcggcgtacc ctcgcgccac atccacagcc acggctcgat tatccatcgc gacgattacg
     7201 ataatgcggt aaaattggtg acggctttgg tggccaagct cgacgcggct accgtggccg
     7261 aattaacact gtaacagaat gacacttacg cacgagcgcg ggggagaaag ggcgaaaggg
     7321 agaaagggtg atacccccat gacgggactt caccccttct cccctgctcc ctttctcccc
     7381 tgcccgtgcc tcgaaacgat aaacaataac tgacaccttt aggaggtcga tcgtacatgg
     7441 caacgcttgc ccctgatttt cagcgcatcg ctgattcctt actggatcag atcaaagcat
     7501 ttgatcacaa agtcacgccg cgcagcgtcg gcaccgtctc ggccgtctat gacggcgtgg
     7561 ctctggccac cggtttggcc gatgtggccg ccaacgaatt ggtcgagttt agcaacggcg
     7621 tagccggtat cgcccttgac ctgcagcccg acaacgtcgg tatcgtcgtc atgggcgact
     7681 acaccggcat cgaagtcggc gacgaagtgc gcgccaccgg caacatcgcc tcagtccccg
     7741 tcggcgaggc catgatcggc cgcgtcgtcg atccgctggg caaccccatc gacggcaaag
     7801 gccccatcaa taccaccaag cgccgcccgc tgcagcgcac cgcccccggc gtcatcgagc
     7861 gcaagggcgt cgacacgccg gtgcagaccg gcatcctctc catcgactcc atgttcgcca
     7921 tcggccgcgg ccagcgcgaa ctgatcattg gcgaccgcca gaccggcaag accgccatcg
     7981 ccctcgatac gatcatcagc cagaagggcc agaacatgat ctgcatctac gtcgccatcg
     8041 gccagcgcgc cgggcaggta gcgcaggttg tggccacgct ggagaagtac ggcgcgatgg
     8101 attacaccac tgtggtcatg gccggcgcgg ccgaccccgc cccgctgcaa tacttcgccc
     8161 cctactccgg ctgcgccatc ggcgaagagt tcatggagaa cggcaaggat gcgctggtgg
     8221 tctatgacga cctctccaag cacgcctggg cctatcgcca gatgtccctg attctgcgtc
     8281 gcccgcccgg ccgcgaggcc taccccggcg acatcttctc gctccacagc accctgctgg
     8341 agcgcgccgt ccgcctgcgc gatgagtacg tcatcgtcga gaagggagcc aatgtgaccg
     8401 ccaagaccaa gggtgtcgac ggcaaggtct acttcggcaa cctcgtcgat gaggaactcc
     8461 acaaggcgtt cgaagccctg ggcgagggcg ccaaggacaa gtacgaggcc aggaagatcc
     8521 ccggcaccgg tggctcattg accgctctgc cgatcatcga gacgctgctc ggcgacgtgt
     8581 cggcctacat tccgaccaac gtcatctcca tcaccgacgg ccagctcttc ctggaaaccg
     8641 acctgttcaa cgcgggccag cgcccggcca tcagcgccgg tttgtcggtg tcgcgcgtcg
     8701 gttcggccgc ccagactcgc gccatgagca aggcttccgg tacgctgaag ggcgacctgt
     8761 cccagttccg cgagttggcc gccttcgcct cgttcggctc cgacctggac gcctctaccc
     8821 agcgccagtt ggcccgcggc gagcggctca tggagctgct gaagcagccg caataccagc
     8881 ccattcgcct cgatcacgag gtgttcatga tctacgccgg cactcgcggc tatcttgaca
     8941 acgtggccgt caaggaagta cagcgctgga aggcggagat gtcccgcttc atggacacga
     9001 cctaccccga agtcggccgc aatatcctgg agacaggtgg ctggaacaac gacatcgagg
     9061 cggccgtgaa gcaaggcatc atcgacttca acaatacctg gagcaactaa tgacgagtga
     9121 cgaacggcga gtgacgaatg acgaattcag ggagattctt cattcgtaat tcgtaattcg
     9181 taattcgtaa ttaaatcatg gcaactgaac gcgaactaaa caaacgcatc aaaagcgtta
     9241 aaaacatatc ccaggtgacg agcgccctgg cggccgtgtc cgcgtcgaaa gccagccgcg
     9301 cccagcgcca ggccgaagcc acccgccact atgcccaaaa ggcgttcgag atcatccaga
     9361 acctcgccgc ccagcccggc gcgtcggcca ccacccaccc gctcatgctc gcccgctccc
     9421 aggtgcgcaa catcggcctg atcctcatgg tcagcgaccg cggcctggcc ggctcctaca
     9481 acgtcaacat gatcaccctg gccgaccgct tcattgctgc cgccggcaag ccggtcaaga
     9541 tcattaccgt cggccgcaag gggcgcgacg ccatggtgcg gcgcggccgc gacgtcgtcg
     9601 ccaccttcga ccgcatcccc gacccgccgg ccttcctcga catcaccgcc gtcgcccgcg
     9661 ttgccatcga cagcatgctc agcgaagacg tcgaccaggt gttcatcggc tacagcgaat
     9721 ttgtcaacat ggtcacccgc cagccggtca ttcgccaatt gctgccgctg cgccccgccc
     9781 accccgacga aatacccggc gcggaatacg tcagcggcgt cgacgcggcc aaggcctcgc
     9841 aacaatccta cacctacgag ccgagcgccg aagccctgct ccacgacatc gtgccccgct
     9901 tcacgcggct gcaggtctac cagtccctgc tcgaggcgtt cgccagcgag cacagcgccc
     9961 gtatggtggc catgaacaac gccaccgaca acgccgccga actggtggac atcctgacgc
    10021 tggagcgcaa caaggcgcgc caggcgtcca ttaccaatga aattctcgac atcgtcgggg
    10081 gcgccgaggc gctgaagaat atgtaagaca atgatcagta agtcagtaga tcagtaggcc
    10141 ggtaggccgg taagtcagta gatcagttac tgaccactga atactaaccc actgaatact
    10201 gacccactga tctactgaat actgaatctg gagacacaac acatggcaac aggaaaaatc
    10261 accgccatcc ggggcgtcgt cgttgacgta gaatttcccg aaggggagct gccggaaatt
    10321 tatgaagcgc tccacgtgga agcgccccag ggccaactcg tcctcgaagt gcagcagcac
    10381 ctcggcggcg gcgacgtgcg caccgtcgcc atgggttcca ccgacggcgt gccgcgcggc
    10441 cgcgatgtgg tcgccaccgg cgggccgatc atggtgcccg tcggcccggt gaccctcggc
    10501 cgcatcttcg gcgtcaccgg cgaggccgtc gacggccgcc ccacgccaaa ggccgacacc
    10561 tactatccca tccatcgcgc cgcgcctgag ttccaggacc aaagcacggc catcgagacc
    10621 ttcgagacgg gtctgaaggt cgtcgacctg ctggccccgt tcatcaaggg cggcaagacg
    10681 ggcatctacg gcggcgcggg cgtcggcaag acggtcacca tcgccgagct aatccgaacc
    10741 gtggcccagg agcatgaggg cgtgtcggtc ttcgccggcg tgggtgagcg cacccgtgaa
    10801 ggcaccgacc tctactacga aatgcaggag tacggcgtgc aggactccgt cgccatggtc
    10861 ttcggccaga tgaacgagac ccccggcgta cgcctgcgcg tcgccctcac cggcctcacc
    10921 atggccgagt atttccgcga cgaaggccgt gacgtgctgc tgttcatcga caacatcttc
    10981 cgctacgttc tggccggctc tgaaatgtcg gcgcttctcg gccgtatgcc cagcgctgtc
    11041 ggttatcagc cgaccctggc cgccgaaatg ggcgcgctgc aggagcgcat caccagcacc
    11101 cgcaaggggt cgatcacctc aatgcaggcc atctacgtgc ccgccgacga ctactccgac
    11161 cccgcgcccg tggccacctt cgcccacctc gacgccatca ttaccctgga gcgcagcgtc
    11221 ttcgcccgtg gtatcttccc cgccgtcgac ccgctggctt cgtccagccg cgccctccag
    11281 cccgacgtcg tgggcgagtt gcactatcgc accgcccgcg aggtgaagca ggtgctccag
    11341 acctacaagg acctgcagga catcatcgcc atcctcggca tcgacgagtt gagcgaggac
    11401 cagaagctgc tggtggcccg tgcccgcaag atcgagcgct tccttggcca gcccatgttc
    11461 gtggccgaga agttccacgg cctcaacggc caatacgtgc ccacggccga gaccgtccgc
    11521 ggcttccgcg agatcctcga cggcaagcac gacgacctgc ccgaacaagc cttctacatg
    11581 gtcggcggca tggaccaggt tctcgaaaag gccgagcaac tgcgcgccat ggcctaatca
    11641 attacgaatt acgaattacg aattacaaat gaagatcgct tcctattcgt aattcgtaat
    11701 tcgtaatttc taattatgga ggtggatatg cccatccaat gtgacatcat cacccaggaa
    11761 cgcatcgtct tcagcggaca ggttgactcc gtcaacctgc ccggcgccga agggcgcatg
    11821 ggtgtgctgc ccaaccacag cccgctgctg acgacgctcg acttcggcga ggtctacatt
    11881 cgccggagcg gcgcagaaga gtactttgcc atcggcggcg gcttcgtcga gattcagccc
    11941 gatcacgtga ccatcctggc cgactcggcc gaacaggcca acgagatcga catcgagcgc
    12001 gccgaacgcg cccgacaagc ggccgaagag atcatgcgca ccggcgtgaa ggacgatccc
    12061 gatcgcatcg ccgccgtgcg cgcctcgctg ctgcgcgccc aggctcgcgt tgacgtcggc
    12121 cgacgtcgtc gtcgccatca tgccgtgccc atgggcggcg gccatcatcc ggacgatcag
    12181 gacaaaaact aggaagggat ccgcagattt gaagaaagtg gtcagtgggt cagtattcag
    12241 taggtcagtt actgatctgc tgacctactg aatactgacc tactgaccta ctgatcaccg
    12301 cgacttctgt cgcgcgacca ctattctcta tctgtgtaat ctgtgaaatc tgtggattct
    12361 tccactcttt acggtacgat tagcccgtgt cattattcac accacaaatt gcaattgatc
    12421 taggtacggt taacgtcctg gtccacgtcc aggggcgcgg cgtcgtgctt caggagccgt
    12481 cggtcgtcgc cattcgtgag gacggcaacc ggacgatcat cgtcgaggtc ggccgcgctg
    12541 ccaaagagat gtacggccgc acgccgggcg agatcgaggt gatgcgcccg ctgcgcgacg
    12601 gcgtcatcgc cgactacttc gtcacccagg ggatgctgga atacttcatc accaaggtgg
    12661 ccgggcgcat ccccttgcgc aagccgtcgg tgatgattag cgtgccctat ggcgtcacca
    12721 gcgtggagcg gcgcgccgtg cgcgaggccg cgctggccgc cggcgctcgc gaggtcaacc
    12781 tgattcccga accgctggcc gcatccatcg gcgcgggtct gcccgtgggc acgccgaccg
    12841 gcaacatggt cgttgacctg gggggcggtt ccactgaggc ggccgtcgtg gccatgaacg
    12901 gcatcgtcgc tgccgaatcg gtgcgcgtag gtggcgtcca cctcgacgaa gccatcatca
    12961 gctacgtccg caagaagtat aacctggtca tcggcgagcc gacagccgag gcgatcaaga
    13021 tcaagatcgg cgcggccatg gagttggacg aacgactgga gatgcaggtg cagggacgtg
    13081 accaggtggc cgggctgccg cgcaccatca ccatgacctc cagcgaagtg accgaggcca
    13141 tcgtcgagca gcttcaggcg atcatcaatg tcacccgtgc cgtgctggcc aagactcccc
    13201 ccgaactgtc gtcagacatc atcgaccgcg gcatggccct gaccggcggc ggcgcgctgc
    13261 tgcgcgagct ggataccctg ctgaccaagg agacgggcgt ccccgcctat gtggccgaca
    13321 accccatcgc ctgcaccgcc ctcggcgcgg gcaaggcgct ggcggagctg ccggtgctgc
    13381 agcg
//
LOCUS       DAOMQG010000055        13329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_1999_length_13329_cov_11.257345, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000055 DAOMQG010000000
VERSION     DAOMQG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13329
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_13329_cov_11.257345"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PLC06_05010"
     CDS             <1..1202
                     /locus_tag="PLC06_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89837.1"
                     /translation="VVGAPMRDGLGLVTLHNVLNAGRIVGEPVAGRAYQLTVNPAPLV
                     AEAKTVASISPLKLSAEWDVSVEATADIPEGLSIKGFGMSKIRALTDQPISQNDPSNA
                     CTAQWVEEVVTDDSALLEVTMTAASSALDVDLYLALDGGDGVFDCIDETLVASSATTG
                     TNESVSMTFPPDGRYWVIAHGWSVPGGSGRFDATIKNIQGNNVTVTDLPTGPVVAGEP
                     ITFGLEAMVDHEPGVELSGLLFIGPADAPMAMSLPITVTIPEPSAGELLTRFSATPDT
                     LKTGEQAAFSLWVSNQGTDEEGASVTISVPAGLVAVPGSVESTIGQAHHNVFERSITW
                     QGVLKAGESVTITFDATASTMSGQVDVGAKVTGAVRGTEIRSSVPVWINTARPPAYIF
                     APMVTGN"
     gene            complement(1395..2228)
                     /locus_tag="PLC06_05015"
     CDS             complement(1395..2228)
                     /locus_tag="PLC06_05015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRO89838.1"
                     /translation="MQAPDQQKTLEIAVGIAREAGALLLDGFGREKRIEKKSSALDWV
                     TQYDKASETLIVERLRQAFPDHALVGEEGTDTGGQGDYRWYIDPLDGTVNYAHGYPMF
                     CVSMGLYHGDDPVLGVIYDPLRQECFTAIAGQGAYLDRPQGSRRLQVSRQTELVAALV
                     MTGFPYDVHTSPENNLNYMSAFVRSAHGIRRSGSAALDAAYVAAGRLDGYWELKVYCW
                     DMSAAVLIVQEAGGRVTFLDGRPFRLERRFDVLISNGHLHEQMQAVVNSAGATSSSSD
                     I"
     gene            complement(2242..3963)
                     /gene="ptsP"
                     /locus_tag="PLC06_05020"
     CDS             complement(2242..3963)
                     /gene="ptsP"
                     /locus_tag="PLC06_05020"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRO89839.1"
                     /translation="MAQTVTGIPSSPGIAIGPTWIFNPPKADVHAYNIANPEVEWRRV
                     NAAIATAIEQLGSLNTRARTTIGSAEAAIFEAHQLLLQDEDLLGEIQQLIETRRINAE
                     AAVHEAIEANAGALLELEDEYFRARAADLRDIGRRVIYALMGIAPDSLRTPTEKSIII
                     AEDLTPSDTVQFDPEYVLGLCTIRGGPTSHSAILARSLGIPAVVSAPLEREGMANGTP
                     AILNGNNGRITLFPTAAQLEEAQRARAAYLGRLADDTAQRDLPAITLDGHRVEVVANI
                     GNAEDARRAIAKGAEGVGLFRTEFLYLDRDTLPSEREQIDAYRSVADVVGSERPLVVR
                     TLDIGGDKAVPYLGFAEEANPFLGWRGIRLIDHHADVLIGQLRALLQACVGTDLRIMV
                     PMVSSIEEVNRARQLLEEAQAGLSAERRPQVEKVQFGVMIEVPSAAIISDQLAEVVDF
                     FSIGTNDLTQYTLAVDRTNERVASLATPFHPAVLRLIARTIENAHAAGKWVGLCGEFA
                     AHPLAVPILLGLGLDEFSMAPSAVPTIKAAIRRLELARCRDIARIALNLPTAAAVEEY
                     LHSIVGV"
     gene            complement(3966..5333)
                     /locus_tag="PLC06_05025"
     CDS             complement(3966..5333)
                     /locus_tag="PLC06_05025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRO89840.1"
                     /translation="MAKEVIMPKFGFTQEDSQIVEWLVKEGDTVEAGDPLLEVTTDKV
                     NMEVEAPASGILAGIRAVAGDTVPVTQIIAYILAPGEALPAYDAPPSAVSAPATTANA
                     EETAPASPAGVGTTSVAARFAAAMGVDTSQVPGTGPGGRVTKQDIEDFLARQPQPSGK
                     VRATPAARRIARETGLDLADVKGSGPRGRVQAHEAAAAATAMTAFPTAAPIATPIIES
                     SAAASSRGELVRVPFTNMRRTIARTLQRSMQEAPHITFQADIDMTNLQALVARANELR
                     PSDMETVTLTAALTRAVAWCLGRHPTINSHIGDDEMIIWPVANIGIAVALEDGLLVPV
                     VKDAGAKGIGQLSDEIRDLSRRARQGKLRPDELADGTFSISNLGMMGIDRFTAIINPP
                     QVAILAVGRTTRMYLPDDQDQPVVRPICTMTLSVDHRAIDGATAARFMADLRRVVQAP
                     ELMVL"
     gene            complement(5373..6359)
                     /locus_tag="PLC06_05030"
     CDS             complement(5373..6359)
                     /locus_tag="PLC06_05030"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRO89841.1"
                     /translation="MRELTYAEALREALRQKMVADERIFLIGEDIGVYGGAFAVTAGL
                     FKEFGAERVIDTPISEAAIAGACIGAALTGMLPVGEIQFMDFVTLSMEQLVLQAAKIR
                     FMFGGKATVPMVLRMPGGSGTGAAAQHSESLENWFVHVPGLKVVMPSSPYDAKGLLIS
                     AIEDENPVIFVEHKLLYRTKGHVPEEMYRVPLSQANVVREGRDLTVVATSIMVSRALE
                     AAERLAQEGIELEIIDPRTLNPLDEKPIVESVIKTGKALVVHEAVKTGGFGGELVSRI
                     VESEAFDYLDAPVRRLAGLDIPIPYNRNLEYHAVPQIENIVEEARKLHQGAY"
     gene            complement(6352..7356)
                     /locus_tag="PLC06_05035"
     CDS             complement(6352..7356)
                     /locus_tag="PLC06_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRO89842.1"
                     /translation="MNDKPLYEALSAEDTARLLREIGPERSREMLQTMMLIRAFEERA
                     QELFSLGRVHGTMHLSIGQEASAVGANFALTNHDYLLNTHRGHGHVLAWGSEVDLMMA
                     EFLGREAGYCRGRGGSMHIADVSRNNLGANGIVGGGIPISVGVGLSIKSRKTDQVCLS
                     VFGDGAANEGAFHESLNMASIWDLPIIYLCENNQYAMSMAVSHAFNIERISQRACAYN
                     IVGVTIDGNDPLAVYQAVGEAKARAQAGNGPTLIEAITYRWKGHSKSDREAYRTRDEV
                     KEWQQRDPIPHFARTLGLSQEEVAAMQAEVVSRIDAAVAFAEASPEPELSTILEGVYA
                     "
     gene            complement(7346..8014)
                     /locus_tag="PLC06_05040"
     CDS             complement(7346..8014)
                     /locus_tag="PLC06_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRO89843.1"
                     /translation="MPKSAVIETYQYLTGSRGRRGGAGNAAVGRQVRETIIPRRMREA
                     FIAHARLVVDHGLTAGTLTEMSQRLDEALFIITAPGSWAATFGEDDLRIGAIHEQKSL
                     AQEEMPRHAQWHRLIYGAGVADAVLLSQPPAALTAAALRLVPAPELLVDAAADIGSLA
                     VFEIVDPAVLPSDDELVGRHALLLPGYGLLSWGDDLSQAIARAEMVNRWCDIMLRSCK
                     FNER"
     gene            complement(8030..9337)
                     /locus_tag="PLC06_05045"
     CDS             complement(8030..9337)
                     /locus_tag="PLC06_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HRO89844.1"
                     /translation="MNTLRDRLRSYEAKYGPIRVGLVGAGQMGTGLMSQMEKMNGMKV
                     MAVADILPGRPAAAYVEAGVAAGQVHETDDPVRAARILQEGGRVATTSSGILAQIDNI
                     DLVVECTGVPEVGAAVCYDAIMAGKNIVNMNVEADATIGYYLAQLAQERGVVYSLVAG
                     DEPGSIKELYDFADTLGFEIVTIGKGKNNPLDRTCNPDHVAEKARRNNMSAKMLASFV
                     DGTKTMVEMTSVGNATGFAPEVRGAYGPQCTVKDLPKVFVPKADGGIFNARGAVDYAV
                     GDVAPGVFIIITTDQPKIIADLRYLRLSGYGDYWAIYRPYHLANLETPITIANVALDG
                     RPTALTTRPPVAETIAVAKRDLMPGDTIDSLGGYTAYGMIDKAGTAAEEGIVPLGLVV
                     GAKVLRPIRMGEPIHYDEVQLNESQTIVELRRRQDEMVRQAAA"
     gene            complement(9345..9704)
                     /locus_tag="PLC06_05050"
     CDS             complement(9345..9704)
                     /locus_tag="PLC06_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HRO89845.1"
                     /translation="MIKFEGRVTAIGPYVSEFIDAGILVLFGQGAPEELAEFAILHDG
                     TTLQGELRAGDTVCVDEACFQVLAVGEVAGANLSNLGHLVLKFNGEHEVEMPGDVCVE
                     AGRIPEVIIGTIIRVEG"
     gene            complement(9712..10095)
                     /locus_tag="PLC06_05055"
     CDS             complement(9712..10095)
                     /locus_tag="PLC06_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter"
                     /protein_id="HRO89846.1"
                     /translation="MSEEKQYRKVKVTAGKRGWGGPLVIEPKPDKPYIYCVTGGGIHP
                     IAQRIADLTGGEAFDGFKSKRDFSEIAVAVIDCGGTARIGVYPMKGVLTIDIHATKPS
                     GPLFRFIKAENFVSGVGPDDIQLID"
     gene            complement(10185..10745)
                     /locus_tag="PLC06_05060"
                     /pseudo
     CDS             complement(10185..10745)
                     /locus_tag="PLC06_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            complement(10767..11315)
                     /locus_tag="PLC06_05065"
     CDS             complement(10767..11315)
                     /locus_tag="PLC06_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HRO89847.1"
                     /translation="MDFLVRLAEGFIGLFQQGGEVFVSLVTGIVPLLIILLTFVNALI
                     ALIGTERIDRVGEWAARPGLVYYPVRYILLPFLSVFFLTNPMAYTMGRFLPERYKPAF
                     YDAAVSYVHPPTGIFPHINPGELFVWLGISAGITALGLSVVPLAVRYLLIGLVVIFIR
                     GIVTEILTSRMWSSVHTDAEGA"
     gene            complement(11446..11877)
                     /locus_tag="PLC06_05070"
     CDS             complement(11446..11877)
                     /locus_tag="PLC06_05070"
                     /inference="COORDINATES: protein motif:HMM:NF018611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HRO89848.1"
                     /translation="MKELVDALATNAGFFFVLLAIMWGLQFVLAFYQLRRFNRRIFEV
                     RKHGKTAVGLSGGRYSGRTYAILAIDETGHIVHAEHFAGATIFARLRPVPDLIGMTLD
                     DMRAALPQLPLSGKSQKSFINAADYLQEAPKVVQDVETQPA"
     gene            complement(11877..12155)
                     /locus_tag="PLC06_05075"
     CDS             complement(11877..12155)
                     /locus_tag="PLC06_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRO89849.1"
                     /translation="MSTITITIEHEAGLHARPLAKFVKVVKQYDATVEVTNLTREKGP
                     VNGTSPLKLMLLAVSQGHTIEISAQGPQADEVLQALRLLIENNFEGEE"
     gene            complement(12190..13143)
                     /locus_tag="PLC06_05080"
     CDS             complement(12190..13143)
                     /locus_tag="PLC06_05080"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HRO89850.1"
                     /translation="MSFERLKLLASVAELYYVEGCGQAEIASRYGYSRSAISRLLAEA
                     RENRLVEIRINHPIQRDTELEYTLRDKFALDSVFVAQRGNLSYERTLALLGRIAAGYL
                     EGVLNNDDIVSISWGTAVYETVSALRPRRLRGTKVIQMIGGLGIGDSQIDGPGVAFRM
                     AASLGAQCYTLNAPLIAADRRTRDSLLSLRAVRDALALALMADYALVGIGSIDQARAS
                     LLRSGYLNLAELEAIQVTGAVGDICGTHFDAEGHILDIDVNSRVVGISLPELRSGPCK
                     VVAVSGGKVKAPAIAGALKGKLIDILVTDSNAAEEVLSLTL"
BASE COUNT         2906 a         4013 c         3711 g         2699 t
ORIGIN      
        1 aagtagtcgg cgcgccgatg cgcgacggtc tcggcctcgt caccctccac aatgttctca
       61 atgccgggcg tatcgtcggc gagccggttg ccggtcgcgc ctatcaactg actgtcaatc
      121 cggcaccgct cgtggcggaa gcaaaaacgg tggcttcaat tagtccgctg aagcttagcg
      181 ccgagtggga cgtctccgtg gaagcgacgg ccgatatacc ggaaggcctg agtatcaagg
      241 gcttcggaat gagcaagata cgcgcgctca ccgaccagcc catcagccag aacgatccgt
      301 ccaatgcctg caccgcccaa tgggtggagg aagtcgtcac cgacgatagc gcgcttctgg
      361 aagtaaccat gaccgccgca tcttccgctc tcgatgtcga cctctatctt gcgctcgatg
      421 gtggtgacgg tgtgttcgat tgcattgacg aaactctcgt cgcctcatcg gccacgaccg
      481 gcaccaacga aagcgtgagc atgacgttcc cgcccgacgg ccgctactgg gtcatcgccc
      541 acggctggag cgtccccggc ggctcaggcc ggttcgacgc gaccatcaag aatatccagg
      601 gcaacaacgt caccgtcacc gatctgccga ccggccccgt ggtcgcaggc gagccgatca
      661 cgttcggtct ggaagccatg gtcgatcatg agccgggcgt cgagctaagc ggcttgctgt
      721 tcattggccc ggccgacgcg ccaatggcaa tgtcgctgcc catcacagtc accatacctg
      781 aaccgagcgc gggcgaattg ctcacgcgct tctcggctac gcccgatacg ctgaagaccg
      841 gcgagcaagc cgccttctcg ctgtgggtgt cgaatcaggg aacggacgag gaaggagcga
      901 gcgtgacaat cagcgtgccg gccggtctgg ttgctgtccc cggctcggtc gagtctacaa
      961 tcggacaggc ccatcacaac gtctttgagc ggagcatcac ctggcaaggc gtcctgaagg
     1021 cgggcgaaag tgtgaccatc accttcgacg ccaccgcctc gacgatgagc ggtcaggtcg
     1081 atgtgggagc caaagtgacg ggcgcagtgc gtggaacgga aatccggtca tccgtgccgg
     1141 tgtggatcaa caccgcgcgg ccgccggcgt atatctttgc tccaatggtc accggcaact
     1201 agccggagcg gtttcgatgc cgccgacctt catcggccgg cggcatcagg ccggataaaa
     1261 cagagggctt tacgaatcag gcgcgaacct gattcgtaaa gcccttttat catacgagcg
     1321 ttttggatat acgtgcaggg atttccacac tcagatctgc ggacaacagg cccgccgatt
     1381 cctgtcgccc gatgtcatat atctgacgac gaagaggtgg cgcctgcgct attgaccacg
     1441 gcctgcatct gctcatgtaa atggccgttg ctgatcagca cgtcaaagcg acgctccagt
     1501 cgaaagggtc ggccgtcgag aaaagtaacg cggccgccgg cttcctgaac gatcaggaca
     1561 gcggccgaca tatcccaaca gtaaaccttc aactcccaat agccatccag ccggccggcg
     1621 gcgacatagg ctgcatcgag ggcggccgaa ccggagcgcc gaataccatg ggcgctgcga
     1681 acaaaggcgc tcatgtagtt gagattgttt tccgggctgg tatgaacatc ataggggaaa
     1741 ccggtcatca ccaacgccgc caccaactcc gtctgccggc tgacctgtaa ccgccggctg
     1801 ccttgtggcc ggtcgagata ggcaccctgt ccggcaatag ccgtgaagca ctcctgccgc
     1861 aatggatcat agatcacccc caggacggga tcgtcgccgt gataaaggcc catcgacaca
     1921 caaaacatcg gatagccgtg ggcataattg accgtgccgt ccaacgggtc gatataccag
     1981 cgatagtctc cctgcccgcc tgtgtcggtg ccttcctctc ccaccagagc atggtcgggg
     2041 aacgcctggc gcagccgttc gacgatcagc gtctccgacg ccttatcata ctgggtcacc
     2101 cagtcgagcg cggagctttt cttctcaatt cgcttctcgc ggccgaagcc gtccagtaac
     2161 agcgcgccgg cttcgcgagc gattccgaca gcaatttcga gtgttttctg ttgatcagga
     2221 gcttgcataa atggtttgat gtcacacccc aacaatggaa tgtaaatact cctcgactgc
     2281 ggccgcagtc ggcagattca gggcaattcg ggcgatatca cgacaacgcg ccagctctaa
     2341 ccggcgaata gcggccttaa tcgttggcac ggccgatgga gccatgctga attcatccag
     2401 ccctaacccc aacaggatcg ggacggccaa tggatgggcc gcaaattcgc cgcacaggcc
     2461 aacccatttg ccggcggcgt gggcgttctc gatagtgcgg gcgatcaatc gcagcacggc
     2521 cgggtgaaag ggggttgcga gtgaagccac gcgctcgttg gtccgatcca cggccaacgt
     2581 gtactgggtc aagtcgttgg taccgatgct gaagaaatcg acaacttcgg ccagttgatc
     2641 cgagataatc gccgccgagg gtacttcgat catcacccca aactgcacct tctcaacttg
     2701 cggccgccgc tctgccgata gcccggcctg ggcttcttcg aggagttgcc ttgcgcgatt
     2761 aacttcctca atactggaga ccatcggcac cataattcgc agatcagtgc cgacacacgc
     2821 ctgcagcaaa gcccgcagct gtcctataag cacgtcggca tggtgatcga ttaggcgaat
     2881 accgcgccag cccagaaacg ggttagcctc ttcagcaaag cccaaatagg ggaccgcctt
     2941 gtctccgccg atatcgagtg tgcgaaccac gagcggccgc tcgctcccca ccacgtcggc
     3001 cacactgcga taggcatcta tctgctcacg ctcgctgggc aacgtgtctc gatccaggta
     3061 caggaattcc gttcggaaca agcctactcc ctcggcccct ttggcgatgg cacgccgggc
     3121 gtcttccgca ttgccgatgt tggccacaac ctcaacgcga tgcccatcaa gcgtgatggc
     3181 aggcagatct cgctgggccg tgtcgtcggc caatcgaccc aggtaggcag ctcgggcgcg
     3241 ttgagcttcc tccagttgag cggccgtcgg gaagagcgta atccggccat tattgccgtt
     3301 taaaatagcc ggagtgccgt tggccatccc ctcccgttcc aggggagcgc taaccacggc
     3361 cgggataccc aggctacgag ccaaaatggc agaatgagaa gtcgggccgc cccgtatcgt
     3421 gcataagccc agaacatatt cggggtcgaa ctgcactgtg tccgagggtg tcaggtcctc
     3481 cgcgataatg attgacttct ctgtgggtgt acgcaggcta tcgggggcaa tacccatcag
     3541 cgcatagata acccgtcgac caatatcacg cagatcggcg gctcgggcgc gaaaatactc
     3601 atcttccaat tcgagtagtg cgccggcgtt ggcctcgatg gcttcatgaa cggcagcctc
     3661 ggcattaatt cggcgtgtct caatcaactg ctgtatttcg cccagcaggt cttcatcctg
     3721 taacaacagt tggtgcgcct cgaagatcgc cgcctcggca ctaccaatcg tcgtgcgggc
     3781 acgagtgttg agcgagccca gctgctcgat cgccgtcgca atggcggcgt tgactcgtcg
     3841 ccattcgacc tccggattag cgatattata agcatggaca tccgccttgg gtgggttgaa
     3901 tatccaggtc ggcccgatgg caatgccggg agaagaggga ataccggtta ctgtttgagc
     3961 catttttaca gcaccataag ttcgggggcc tgcacaacgc ggcgcagatc cgccataaag
     4021 cgagctgccg tagctccatc gatagcgcga tgatcaaccg acaaggtcat ggtgcaaata
     4081 ggtcgaacga caggctgatc ctgatcgtcg ggcaggtaca tccgagtggt gcgaccgaca
     4141 gccaggatag caacctgtgg cggattgatg atcgccgtga agcgatcgat gcccatcatg
     4201 cccagattag agatagaaaa tgtgccatca gccaattcat ccggccgcag cttgccctga
     4261 cgggcgcgac gactcagatc gcggatttcg tccgataatt gtccgatgcc tttagcgccg
     4321 gcatccttca ccaccgggac gagcaaacca tcctccagtg caacggcgat gccaatattg
     4381 gcaaccggcc agatgatcat ctcatcatcg ccgatatggc tgttgatcgt cggatgacgg
     4441 ccgaggcacc aggcaacggc acgagtgagg gcggcggtca gcgtgaccgt ctccatgtcg
     4501 gacggccgca actcgttggc gcgtgcgacc agcgcctgca agttggtcat gtctatgtcg
     4561 gcctggaagg tgatgtgcgg ggcttcttgc atgctgcgct gcaaagtccg agcaatagtg
     4621 cggcgcatgt tggtaaacgg tacgcgaacc aattcgcctc gagaggacgc ggctgccgaa
     4681 gattcgatga tcggcgtggc tattggtgcg gccgtgggga acgccgtcat tgccgtcgcc
     4741 gccgcggccg cctcatgggc ctggacacgg ccgcgaggac cgcttccctt tacatcggcc
     4801 agatcaagac cggtctctcg agcgatgcgc cgagcagccg gcgtggcccg aaccttgccc
     4861 gacggctggg gttgccgggc cagaaagtcc tcaatatcct gtttcgtcac ccgaccgccg
     4921 gggccggtcc ccggcacctg ggaagtatcg acacccatcg ccgccgcgaa tcgggcagca
     4981 actgatgtag tacccacgcc ggccgggctt gccggcgctg tttcttcggc gttcgcggtt
     5041 gtagcaggcg cagaaactgc gctgggaggg gcgtcgtaag cgggtaacgc ctcgcccggt
     5101 gcaaggatgt aggcgatgat ttgcgttacc ggcacggtat ccccggcgac agcgcgaatg
     5161 ccggccagga tgcctgatgc cggtgcctcc acttccatgt tgaccttatc ggtcgtaacc
     5221 tcaagtaatg ggtcgcccgc ctcaacggta tcaccctctt tcaccagcca ttcgacgatc
     5281 tgggaatctt cctgggtgaa cccaaatttg ggcataataa cttcttttgc catcctgata
     5341 cctcacgcag cgtgctatag atcgcggcgc gatcagtatg cgccctgatg cagcttccgg
     5401 gcttcctcga caatattctc gatctgcggc accgcatgat attccaggtt ccggttatag
     5461 gggatcggga tatcaagccc ggccaggcgg cgaacgggag catcgagata gtcaaacgcc
     5521 tcgctttcga caatacgcga gacaagttcg ccgccgaaac cgcccgtctt gaccgcctca
     5581 tgaacaacca gcgccttacc ggtcttgatc actgactcga cgatcggctt ctcatccagc
     5641 gggttgaggg tgcggggatc gatgatctcc agttcgatgc cctcctgagc cagacgttcg
     5701 gccgcttcca gggcgcgcga caccataatg gaggttgcca caaccgtcag gtctcgccct
     5761 tcccggacga cgttcgcttg agaaagcggc acacggtaca tctcttcggg cacatgaccc
     5821 ttggtgcgat agagcagctt gtgttcaacg aagatgaccg gattctcatc ctcaatggcg
     5881 gagatgagca accccttggc gtcataggga gaactgggca tgaccacctt caggccgggg
     5941 acgtggacga accagttctc cagactttcg gaatgctgcg cggccgcgcc cgtgccggat
     6001 ccgcccggca ttcgcaggac catcggcacc gttgccttgc cgccgaacat gaagcgaatt
     6061 tttgccgcct gcaataccag ctgctccatg ctcagcgtga caaagtccat gaactggatt
     6121 tcgccaacgg gcaacatgcc ggtcagagcc gcgccgatgc aggcaccggc gatagcagct
     6181 tcggagatgg gtgtgtcaat gacccgttcc gcgccgaatt ccttgaacag cccggccgtg
     6241 acggcaaacg cgccaccata aacgccgata tcttcgccaa tcagaaaaat gcgctcgtct
     6301 gccaccattt tctggcgcaa ggcttcacgc agtgcttcag cataggtcaa ctcacgcata
     6361 gaccccctcc aggatggtgc ttagttccgg ctcaggactc gcttcggcaa aagccacggc
     6421 cgcgtcgatg cgcgacacaa cttcggcttg catggcggca acttcttcct gcgacaatcc
     6481 caacgtacgg gcaaaatggg gaatgggatc gcgttgctgc cactccttca cttcatcgcg
     6541 cgtgcgatag gcctcgcggt cgcttttgga atgacccttc caccggtaag tgatggcctc
     6601 aatcagcgtc gggccattgc cggcctgggc gcgagctttg gcctcgccca cggcctggta
     6661 gacggcgagc gggtcgttgc cgtctattgt cactcctacg atattatagg cgcaggcacg
     6721 ctggctgatg cgttcgatat taaaagcgtg gctgacggcc atggacatgg cgtactggtt
     6781 attctcacac agatagatga tcgggagatc ccatatgctg gccatattga gcgattcatg
     6841 gaacgccccc tcattggccg cgccgtcgcc aaagacgctc aggcaaacct gatctgtctt
     6901 gcgagattta atgctgagac cgacaccgac cgagatggga ataccgccgc cgacaatgcc
     6961 gttggctccc aggttgttgc ggcttacgtc ggcaatgtgc atcgagccgc cgcggccgcg
     7021 gcagtaaccg gcctctctgc ccaggaattc ggccatcatc aggtcgactt cactccccca
     7081 ggccaggaca tgtccatgcc cacggtgggt attgagcaga tagtcgtggt tggtcagcgc
     7141 gaaattagcg cccaccgccg aggcttcctg cccaatggaa agatgcatcg tgccgtggac
     7201 acgccccaac gagaacaact cctgtgcccg ctcttcaaat gcccggatga gcatcatcgt
     7261 ttggagcatc tcacgactgc gttcggggcc gatttcgcgc aacaatcgcg ccgtatcttc
     7321 tgcgctgagc gcttcataga gtggtttatc gttcattgaa tttacatgat ctcaacataa
     7381 tatcgcacca gcgattgacc atctccgcac gagcaattgc ctgtgacaag tcgtcgcccc
     7441 aactaagcag gccatagcct ggaagaagca gcgcatgtcg gccaacaagc tcatcatcgg
     7501 acggcaaaac cgccggatcc acaatctcaa acacggcgag ggaaccaatg tccgcggccg
     7561 cgtctaccaa caactcaggt gccggcacca gtcggagcgc agcagcggtc agcgcagccg
     7621 gaggctggct caacaggaca gcatcagcca caccggcgcc atagatgagt cgatgccatt
     7681 gggcatggcg cggcatctct tcctgagcca ggctcttttg ctcatggatc gcgccgattc
     7741 gcagatcgtc ctctccgaag gtggccgccc aactccccgg cgcggtgata atgaacagcg
     7801 cttcatccag gcgttggctc atttccgtca acgtgccggc cgtcagccca tggtcaacca
     7861 ccaaccgagc atgagcaatg aaggcctccc gcatccgccg cgggatgatc gtctcgcgca
     7921 cttggcggcc gacggccgcg ttgccggctc cgccgcgcct gccccggctt ccggtcaggt
     7981 attggtaggt ttcaatgacc gcggattttg gcatcgtacc agctcatgtt caggcagccg
     8041 cctgacgcac catttcatcc tgccggcgcc gcaattcgac gatggtctgg ctctcgttca
     8101 gttgaacttc atcataatgt atcggttcgc ccatcctgat ggggcgcaaa actttggcac
     8161 cgacgaccag acccagaggg acaatccctt cttccgcggc tgtgccggct ttatcgatca
     8221 tgccgtaggc cgtgtagccg ccaagtgaat cgattgtatc gccgggcatc aggtcgcgtt
     8281 tggcgacagc gatcgtctcg gcaaccggcg gccgcgtcgt caaagcggtt gggcgaccat
     8341 ccagggccac gttagcgatg gtgatgggtg tctccagatt agccaggtga tagggacgat
     8401 agatggccca atagtcgccg tagccgctca aacgcagata gcgtaaatcg gcgatgatct
     8461 ttggctggtc ggtggtaata ataatgaaga cccctggcgc aacatcaccg acagcgtaat
     8521 cgaccgcgcc gcgggcgttg aagataccgc catcggcctt cggcacgaat actttcggca
     8581 gatccttcac agtacattgc ggcccatagg cgccgcgcac ttcaggagca aagccggtcg
     8641 cattgccgac agatgtcatt tcgaccatcg tcttggtgcc gtccacgaag gaagccagca
     8701 tcttggcgct catattgttt cggcgcgcct tctcggcaac gtggtcggga ttgcaggtgc
     8761 ggtcgagcgg gttgttcttg cccttgccga tggtcacgat ctcgaaacca agcgtgtcgg
     8821 cgaaatcata tagctccttg atcgaacccg gctcgtcgcc ggccaccaga gagtagacta
     8881 cgcctcgctc ctgggccagt tgggccagat agtagccgat agtcgcatcc gcctcgacat
     8941 tcatattgac aatgtttttg ccggccataa tcgcatcata gcagacggcc gcgccgactt
     9001 ccggcacacc ggtacattca acgacgagat cgatattatc gatctgggcc aggatgccgc
     9061 tggaggtagt ggcaacacgg ccgccctcct ggaggatgcg agcagcccgg acaggatcgt
     9121 ccgtctcgtg gacttgccca gcggcgacgc ctgcttcaac ataggcagcc gcgggacgtc
     9181 ccggcaatat gtcggcgacg gccatcactt tcatcccgtt catcttttcc atctggctca
     9241 tcagaccggt gcccatttgg cccgccccaa caagcccaac ccgaatcggg ccgtattttg
     9301 cttcatacga ccgcaaccga tcgcgcagcg tattcatggt tttcttatcc ttctacacgg
     9361 attatcgtgc cgattatcac ttcaggaatc cggcctgctt ctacacatac atcaccgggc
     9421 atctcgacct catgttcacc attgaacttc aacaccaaat ggccaagatt cgacagattc
     9481 gcgccggcaa cttcaccgac agccaacacc tgaaagcaag cttcgtcgac gcaaacagta
     9541 tccccggccc tcagttcgcc ctgaagcgtt gtgccgtcat gaagaatggc aaactcggcc
     9601 aactcctccg gcgcgccctg gccgaagaga acaagaatcc cggcatcgat aaattcagat
     9661 acatatggcc caatggccgt cacgcgacct tcaaatttga tcacggctat cctaatcgat
     9721 taattgaatg tcgtccgggc caacgccgga aacgaagttc tcagctttga tgaagcgaaa
     9781 cagcgggccg gatggcttgg tcgcatgtat gtcaatggtt agcacgccct tcatcggata
     9841 gacgccgata cgcgccgtgc cgccacagtc gatgaccgcc accgcaattt cactgaaatc
     9901 acgctttgat ttgaaaccat caaacgcttc accaccggtc aggtccgcga tgcgctgggc
     9961 gatcggatga atacctccgc ccgtcacaca gtagatatag ggcttatccg gcttcggctc
    10021 aatgacgagc ggccctcccc atccgcgctt gccggcagtg acttttactt tacgatactg
    10081 cttttcttcg ctcacaggtt tgactcctta tctatctggg ttgaacatgg aaccgggcca
    10141 agcgataagg ctcgcccggt tccatcaaat cggatcaacg aattttagta ccccggatac
    10201 agcccgaagc tggcaagcca ggccaggatg accgcaaccg gaccggtgat taaccgggag
    10261 aacaaaatag ccggaacgcc tacctcgacc gtctccggtt ccgcctcgcc aagggccagg
    10321 ccgaccggga tgaagtcaca gccgacctgt gggttgatgg cgaacaacgc cggcagggcc
    10381 aggtgggcgg acatcgaacc gctggcgatc agcgtactgc cgatcagcgt gccaacgacc
    10441 tgagcgataa cggcgcccgg ccccaacagg ggggacagga ccgggatagc acagatgatg
    10501 gagatgatga tcaatcccca gatattgccg gccaacgggg ccagcgcatt ggcgatccag
    10561 tcaccaacgc cggtcttgag aatgatgccg ataaccaggg cgataaacgc catgaacggc
    10621 agaatgttga cgatcacctg gtcgattgcc tcgcggccgg attgatacaa agtcccgacg
    10681 acattgccga caccacgacc aagtttggtg agaaatgagt taagtgcttt catcgctttc
    10741 ctctctagtt ccttatcctg gggtgattag gcgccttctg cgtcggtatg aacgctggac
    10801 cacatgcgac tggtaagaat ttcagtcacg atgccgcgga taaaaatgac cacaaggccg
    10861 atcaacagat agcggacggc caagggaaca accgatagac ccagcgcggt gatgccggcc
    10921 gagatgccca gccagacaaa caactcgccc ggattgatat gcgggaaaat acccgtcggc
    10981 gggtgaacat aggagacggc cgcgtcatag aacgccggct tgtaacgctc ggggaggaaa
    11041 cgtcccatgg tataggccat cgggttggtc aggaagaaca cagaaaggaa cgggagcagg
    11101 atatagcgta ccgggtagta aacgagaccc ggacgcgctg cccattcacc gacacggtcg
    11161 atccgttcgg tacctatcag ggcgatcaac gcgttgacga atgtcaacag gatgatcaat
    11221 aagggaacaa tgccggttac cagactgacg aagacttcac cgccctgttg aaaaaggccg
    11281 atgaaccctt ctgctaatcg aacaaggaaa tccatttctt acctccgtga ttaaatgttt
    11341 acgcaaaaat tgaagtggtg cttcactgca taaagtatgg ataagtcgcc ctgtatgctt
    11401 tcttcgaacg tataggccct ccttggttaa tgatattgca ggcgatcagg caggctgggt
    11461 ttcaacatcc tggactacct tcggcgcttc ctgcagataa tcggccgcat tgataaatga
    11521 tttttggctc ttgccggata gcgggagttg cggcagcgct gcccgcatat cgtccaacgt
    11581 cataccgatc aaatcgggca cggggcgcaa tcgggcaaag attgtcgccc ccgcgaaatg
    11641 ttcggcatga acgatatggc ctgtctcgtc gattgccagg attgcatagg tacggccact
    11701 gtaacggcca ccgcttaggc cgacagccgt tttgccgtgc tttctgactt caaatattcg
    11761 tcggttgaag cgacgtagct gatagaaagc cagcacaaat tgcaatcccc acataatggc
    11821 caaaagcacg aagaagaaac cggcgtttgt ggcgagtgcg tcaaccagtt ctttcatcac
    11881 tcttctccct cgaagttatt ttcgatcaac aaccgcagcg cttgcagtac ttcgtcggcc
    11941 tgtggcccct gggcactgat ctcgatagta tgtccttgcg agacagccag cagcatcagc
    12001 ttgagtggac tggtaccatt gacagggcct ttttcgcgcg tcagattggt cacttcgacg
    12061 gtcgcatcat actgcttgac taccttgacg aatttggcca aaggtctggc gtgaaggcct
    12121 gcttcgtgtt caatagttat agtaattgta gacatcgctt cctcacatct ccccttctgc
    12181 gccacctcgc tacaaggtca acgaaagcac ctcttcggca gcgttgctat cagtcaccag
    12241 gatatcgatc aatttgcctt tcagggcgcc tgctatcgcc ggcgctttca ccttgccgcc
    12301 gctgacggcg acgaccttgc atgggccgga gcgaagttcg ggcagcgaga tgccaactac
    12361 gcggctattc acatcgatat cgaggatatg accctcagcg tcgaaatgcg tgccgcagat
    12421 atctccaaca gcgcccgtaa cttgaatggc ctccagttcg gcaaggttca gatagcccga
    12481 ccgcaacaaa ctcgcgcgag cctgatcaat actgccgata cctaccagag catagtcagc
    12541 catgagggcc agagccaggg catcccgcac cgctctcaat gacaagagtg aatcgcgagt
    12601 tcgacgatcg gcggcgatga gcggcgcgtt gagggtatag cattgggctc ccaatgatgc
    12661 tgccattcga aaagccacgc ccggcccatc aatttgcgaa tcgcctatac ccagaccacc
    12721 gatcatttga atgactttgg tgccacgcaa cctccgtggt cgcaacgcgc tgaccgtctc
    12781 atagacggct gtgccccatg atatgctgac gatatcgtcg ttattcagca cgccttccag
    12841 ataaccggcg gcaatacgac ccagaagggc caatgtacgt tcataggaca gattgccgcg
    12901 ttgagcgacg aagacggaat cgagggcgaa tttatcgcgc agggtgtatt cgagttccgt
    12961 atctcgctgg atcggatggt tgattcgaat ttcgacaagg cggttttcgc gcgcctcggc
    13021 caataaacgc gagatagcag agcgagagta accgtagcgg ctggcaattt cagcctgtcc
    13081 acagccctca acgtaataga gttccgccac agatgctaat agcttaagcc gttcgaagga
    13141 cattcccttt tgccagttct gcgcaaaacc atctgatttc gcgcgactat accaatcgat
    13201 ggctaaaact ttcccgtaag cagtcgctaa tgttgcttat tctactctaa tgaatttaaa
    13261 acactcacat atatgaaata tattcacata tgtgaatttc cagggactaa cgacaatgag
    13321 ctatacata
//
LOCUS       DAOMQG010000056        13112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2039_length_13112_cov_12.433714, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000056 DAOMQG010000000
VERSION     DAOMQG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_13112_cov_12.433714"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..917
                     /locus_tag="PLC06_05085"
     CDS             30..917
                     /locus_tag="PLC06_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRO89851.1"
                     /translation="MFILEWWFCYLPSTKLTGQTVLEEIMATYLLTWNPLRFDWTDIA
                     EEIAELQEIGWLDGRWSCGNNKSIQPGDRFYLIRLGNQVFNKGIVGSGYIISKYYEAP
                     HWVDGDERPTGPALYVDVRFDTLLDAEVEKILEIEELKNDPILMQMHWSTQRSGVRIQ
                     DNIVEELQRRWSDLQTVRHPVFPLEDTVFSEGQVKQVQLTTHERNAVARQICIEHYGA
                     ACILCGFDFGDCYGPAGEGYIHVHHITPLSEIKEEYYVNPINDLVPVCPNCHAIIHRR
                     LPCYSLQEMRLILNHEHRH"
     gene            972..2348
                     /locus_tag="PLC06_05090"
     CDS             972..2348
                     /locus_tag="PLC06_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRO89852.1"
                     /translation="MHRTSSLIIILLVLLLLPACRSGPPVAPTGEATASGRPSPAGSE
                     TAATPTPTPPGGQIFAPVVPGGEPTGDQPTAAYPALPVATGTPAAPTPVVPTPTPEPE
                     YPVYTGPPLNRDEIGIQIYLHRQDVRDLLRHLEALDVGWVKVQVSWKLHEPRPGEYNE
                     ELFGELDRLIDGANGQNVKVLLSVSKAPEWSRPTTEMDGPPSDYALFEGFMRYLATRY
                     TGKVAAYELWNEPNLQREWNGTPLSAADLVELIRRGAAGVRAVDPAALIISAAPATTG
                     INDGVNAIDDRVYFGGMLAAGVASVIDGAGVHPYGWANPPDSRAADATQVSSSHNNHP
                     SFFFADTLEDYRAMLVAAGAADIELWPTEFGWGTFEGLLTDEGQPASPPPGSEFMADN
                     TEWEQAIYTLRAFEMGHEREWVGPMFLWNLNFTKALGPEFQEVGYSLLRFDDSRRPVY
                     RALERRGE"
     gene            2424..3848
                     /locus_tag="PLC06_05095"
     CDS             2424..3848
                     /locus_tag="PLC06_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO89853.1"
                     /translation="MFVDRQRELAYFDDLLTRQRPGPAQLILLYGRRRIGKTALLLEW
                     AERNAVPHTYWAAEKEPADLQRRKLYARLLNLPIRQAPRLDSWAELWDAVAAIIADRR
                     QILILDELPYAVESDPATLSALQHAWDAHFQHSSLVIVLCGSQVKVMESLQMHQSPLF
                     GRLTGQWYLEPLPFSALRDFFPDWSAEERVAAYAVTGGVPAYLNWLDPALNLTANIRR
                     IILSEGSMFLAEPAFLLYDEVREPQSYLATLKAIGLGSHTLGDISNDALIATSHLSVY
                     LQRLQDLKLVERRLPAATPPAKRRTSRLGRYHLADPYFRFYFRFIAPFHEYLPFDTEQ
                     VLERIRHDLRGFVGGTAFEDLARQWVTQQGKAGTLPLRPGIVGAHWSRKVQIDVVAVD
                     WEARQLLLGECKWGDGNVDRSVVRDLIEGKGELLRKELPDGEAWRFHYALFTRAGLTP
                     AAAAELAKYDGLHVDLARLDGELV"
     gene            complement(3840..4055)
                     /locus_tag="PLC06_05100"
     CDS             complement(3840..4055)
                     /locus_tag="PLC06_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89854.1"
                     /translation="MKPIHSYPIAEARDNLTAPIREAEATVLAEPFSSALGRFREQVD
                     VAALELGPDLFENMRQKSAGREPTASD"
     gene            complement(4157..4975)
                     /locus_tag="PLC06_05105"
     CDS             complement(4157..4975)
                     /locus_tag="PLC06_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89855.1"
                     /translation="MQMTPDNQWIDKIRQQIQAALNDSKRDILREQFGMLHEHRSPDL
                     SPEVESEWLDYILEFERQFENAPRITVSARIGDPAPPPIDALSSDELVAAVDSLMGLL
                     AAHYIAVDFLGVVDYETVYRYLTEELLDEEIDDIRLPDTWINFIYATDEYNVETWVEQ
                     FVDAIFRHDLEYAQDCVDDDQLWADTLGKLLTVAELEALWERMPVVEDVTCRALETQI
                     TGDNGQVFSRITWSADGVTQVIEASFQVHRSRYLDEAWDIEQTSLTNELWRVYG"
     gene            5129..5371
                     /locus_tag="PLC06_05110"
     CDS             5129..5371
                     /locus_tag="PLC06_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89856.1"
                     /translation="MLKNITLSADEALIREARRQAALQNTTLNALFREWLEQYTSEQD
                     ADARRLAAARYDEWMQQLSHVNAGRKFTREEMNERR"
     gene            5358..5798
                     /locus_tag="PLC06_05115"
     CDS             5358..5798
                     /locus_tag="PLC06_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRO89857.1"
                     /translation="MSDAEDTFFLDTNILIYAVDEADLVKQQIAVALVREAILSGRGI
                     ISIQVVQEFLNVARRKFKQPMTIEQCRGHIRNVLEPLCAYFPSISTMDRALLTIDETS
                     YSFYDALILTAAIESGCRTLYSEDLQHGRKIQGLTILNPFIGTE"
     gene            6052..6915
                     /locus_tag="PLC06_05120"
     CDS             6052..6915
                     /locus_tag="PLC06_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRO89858.1"
                     /translation="MKIVTDSAADLTSEDISTFDISVAPLYIQFPDGEVRSGDLSHDE
                     FYNRLEAVRPQLPTTGQPSPGNFSDLYHPIQEQGDGILSIHISSGLSGTVNAARNGAA
                     QENEDSIHVIDSLTLSGGLRFQVLAAAQAIAAGWDVPNIRAQLDRVRTATEVVYTLDT
                     LEYLARGGRIGRVQALASSILNIKPIINVSKSDGKYNTVGKSRTMSRAINDLTEHLAS
                     LYGGKEPLWVSVMHGHFEEKARLLAESLQRILPVEKLEVLRISPVLGVHTGPGIVGAA
                     VVPMRFMKEIH"
     gene            complement(6978..>7368)
                     /locus_tag="PLC06_05125"
     CDS             complement(6978..>7368)
                     /locus_tag="PLC06_05125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89859.1"
                     /translation="GLTDAVVDAGWVALEQLPATLAAADVGLYLMDDTLLNRTKCPVK
                     LADMLAAGVPVVAETVGQVAEYVRDGRTGLLCASGDVAELVNAATILLTDRELRMSRG
                     VAAREDITARFDWPELSRTLEALYDRL"
     assembly_gap    7369..7468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7469..7917)
                     /locus_tag="PLC06_05130"
     CDS             complement(<7469..7917)
                     /locus_tag="PLC06_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89860.1"
                     /translation="MRIAMIGPFGLHPKQTMRSRALGLARPLVDRGHTVRLIMPPWDT
                     PDEADREWQEDGIELRYVSLKGGTLGITRRLVRETLAWRPDVVHTFKPKAYSGLVAEW
                     LWRFHRGRIRLVMDTDDWEGAGGWNDIAPYSPLQKRFFARQERWGLT"
     gene            complement(7990..8703)
                     /locus_tag="PLC06_05135"
     CDS             complement(7990..8703)
                     /locus_tag="PLC06_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRO89861.1"
                     /translation="MSKHTFLRIVRAIARVVLAVIARIEIIGNVDYQTGAYIIAANHV
                     GRLEAFLVMLLAERDDIVMILAEKYKQYAFWRYVARKVDAIWVNRFDADLAALRAVLK
                     RLEAGDMLAVAPEGTRSPTATLLPGKPGAVYLAAKSGAPLIPIGIVGTEDAVVKYRLK
                     RFKRLDITARIGEPYTLPPMPRAGRDEWLQAQTDDLMCRIAALLPPDHRGVYADHPRL
                     LELLRENGSPFPEDLPLHS"
     gene            complement(8748..9644)
                     /locus_tag="PLC06_05140"
     CDS             complement(8748..9644)
                     /locus_tag="PLC06_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO89862.1"
                     /translation="MTESTPVCDYEGSDYRARFWDNQGRDYEDQVERAALRRLMPPSG
                     RTLIDIGAGFGRLADEYRGYDRVVLFDYSRSLLREAQQHLGDDPRYRYVAGNWYTMPF
                     VDGLFDTLVQVRTLHHAADAPALFDELARIAGPQAAYVLEFASKHNLKAMLRYGLGRQ
                     TWSPYEREPVEFVALNFDFHPAWIREQLAESGFAPGETLTASHFRFAPIKRIVPTGWL
                     VALDRAIQGTGAWWQLTPSVFVLSQLTGSTTAANENAFFACPACQTPLGEPVDDLLVC
                     ANPACGRRWRVEDGLYDFKEPV"
     gene            9857..11053
                     /locus_tag="PLC06_05145"
     CDS             9857..11053
                     /locus_tag="PLC06_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRO89863.1"
                     /translation="MANDVAIFLDLDNLVIGARDANLNFDISLLIDHIKKRTGGRVVL
                     RRAYSGNLRQDQKLMKELATAGFTMQAAVPLNNFGKNLIDMYLVVDSMDTLVDGQEYS
                     TYVLVTGDRDFVPLIHALRRRGKHVIGVGLKHTTSESLMGLCDEYVYYEDMLPAPPLS
                     ESEADALLTKAIDGLLAGDERVRASVVKERMIELSGGQFGRFQYSDTSFTKFLGRHAN
                     RIVLEKDGTTTYVRRAVELTAPTELYRRYRSALKKQKLRVIPPRLRLVLLREMIEILQ
                     KSGDTMEWRELIDRLAVLHPGEEEGEASRNMINALIVLARRAGVVRTLKGKTLSTAPV
                     LLALEGPKLFQESVIRCDTVYLEAIRDLPEPFDVEEAAQAIYDQVSYAPYLRQLLARM
                     DAAQVG"
     gene            complement(11172..12890)
                     /locus_tag="PLC06_05150"
     CDS             complement(11172..12890)
                     /locus_tag="PLC06_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRO89864.1"
                     /translation="MSLTYADKPWTKSYDKGVPESIDVPAYPVQHFLEEAARRVPTNT
                     ALVFQGKELTYDELNKASDAIAAGLAANGFKKGDRAVIYMPNLPQFVIIYYGILKAGG
                     IVIATNPLYTERELQHQLKDCGAETVFVLSRYYPLLKKVQKSGGTNVKRIIVAYIKDY
                     LPGIKSLLYGLVKEKKEGDRVDVEPGDMTMKDFMALGARSPKPNVTVTGDDIALLQYT
                     GGTTGLSKGAIGLHRNLVANALMVSSWIDDWKYGADTLLGAIPFFHSYGMVTAVIFTV
                     AIGGRLLIIPNPRDLPYVLESINKYKPAYFPGVPAMYVGINNNKDVAAGKYDLRSIRA
                     CISGSAPLLVETKKKFEELTGGTLVEGYGLTESHVATHANPLKGKNPIGSIGLPLPGV
                     EARIVDPVEGNDELPIGAIGELIMRGPTIMQGYWNMPNETASSLRDGWLYTGDIARMD
                     EEGYFYIEDRKKDLIICGGYNVYPREIEEVLSRHPAVMEVSVAGVPDPKRGENVMAWV
                     VKRPGFESVTEAEIIEWSKGELAAYKYPRLIQFRDELPKTTVGKVLKRELVREYKEKA
                     NGKSKG"
BASE COUNT         2633 a         3693 c         3992 g         2694 t
ORIGIN      
        1 ggccttataa ttaaattaat cgcgccccat tgtttatttt ggagtggtgg ttctgttatt
       61 taccctccac caaacttacc ggtcagacgg ttctcgagga aataatggct acgtatttgt
      121 taacatggaa ccccttgcgt tttgactgga cagatattgc agaagagatc gccgaacttc
      181 aggagatcgg ttggctggat ggtcgttgga gttgtggcaa taacaagagc attcaacctg
      241 gtgatcgatt ttatttgata cggttaggca atcaggtctt caataaagga atcgttgggt
      301 ctgggtacat tatctctaag tattacgagg cccctcactg ggttgatgga gatgaaaggc
      361 caacgggtcc tgcattatat gtggatgtac gatttgatac attgttggat gccgaagtcg
      421 agaaaatttt agagatagaa gaattaaaaa atgatccaat cctaatgcag atgcactggt
      481 caacgcagag atcaggtgtt cgcatacaag ataatattgt agaggaatta caaaggcgat
      541 ggtcggattt gcaaacagtc agacatccgg tatttccact tgaagatacc gtcttttccg
      601 aaggccaggt aaaacaggtt cagcttacga cgcacgagcg aaatgctgtt gctcgccaaa
      661 tatgtatcga gcattatggc gcagcttgta tattatgcgg cttcgatttt ggcgattgct
      721 acggcccagc cggggaaggg tatattcatg ttcatcatat tactcctctg tcggaaataa
      781 aagaggaata ttatgttaac ccaatcaatg atctcgttcc tgtttgtcca aactgccatg
      841 ctatcattca tcgacgccta ccttgctatt ccttacagga aatgcggtta atattgaatc
      901 atgaacatcg gcattagagt atcgccacag caaatgacaa ggaagcgctc gacaacccta
      961 taatcgagaa gatgcatcga acttcttccc tgatcattat tctgcttgtg cttctgctgc
     1021 ttccagcctg tcgcagtgga ccgccggtcg cgccgacggg ggaggcgacc gccagcggcc
     1081 ggccgtcgcc agccgggagc gaaacggccg cgactcccac gcctaccccg cccggagggc
     1141 agatctttgc cccggttgtg cccggtgggg agccgaccgg tgaccaaccg accgccgcct
     1201 atcccgcgct gccggttgcg accgggacgc ccgccgcccc aacgcccgtc gttccgacgc
     1261 caacgcctga accggagtac cccgtctaca ccggcccacc gctcaaccgt gacgagatcg
     1321 gcatccagat ctacctccac cggcaggacg tgcgcgatct gctgcgccat ctggaagcgc
     1381 tcgatgtcgg ctgggtgaag gtgcaggtgt cgtggaagct ccacgagccg cggccgggcg
     1441 agtacaacga ggagttattc ggcgaactgg accgcctcat cgacggggcc aacggccaga
     1501 acgtcaaggt gctgctcagc gtcagcaagg cccccgagtg gagccgcccg acgacggaga
     1561 tggacggccc gccaagcgat tacgccctgt tcgaggggtt catgcgctat ctggccacgc
     1621 gctacaccgg caaggtggcg gcctacgagc tgtggaacga gccgaatttg cagcgggagt
     1681 ggaacggcac gccgctgagc gcggccgacc tggtggaact catccggcgc ggcgcggccg
     1741 gggtgcgggc cgtcgatccg gctgcgctga tcatcagcgc cgccccggct acgaccggca
     1801 tcaatgacgg tgtcaacgct atcgacgacc gggtgtactt cggcgggatg ctggccgcgg
     1861 gtgtggcctc ggttatcgac ggtgcgggcg tccaccccta cggctgggcc aacccgcccg
     1921 acagccgcgc ggccgacgcg acacaggtct catcgagcca caacaatcac cccagcttct
     1981 tcttcgccga cacgctggag gattaccgcg cgatgctggt ggcagcgggc gcggccgaca
     2041 tcgagctatg gccgaccgag ttcggctggg ggacgttcga gggactgctg acggacgagg
     2101 ggcaaccggc gtccccgccg cccggttcgg agttcatggc cgacaacacc gagtgggagc
     2161 aggcgatcta taccctgcgc gccttcgaga tgggccacga acgggagtgg gtggggccga
     2221 tgttcctgtg gaatctcaac ttcaccaagg cgctggggcc ggagttccag gaggtgggct
     2281 atagcctgct gcggtttgac gacagccggc ggccggtgta tcgggcgctg gaacggcggg
     2341 gggagtagtt agtctgctgc tccttgaaat taagctacgt gttgtgtatt atatacagca
     2401 tgtagcttaa ttgaggtgaa gccatgtttg tcgatcgcca acgtgagctg gcttattttg
     2461 atgatctcct gacccgtcag cgaccggggc cggcacaatt gattctcctg tatggccggc
     2521 ggcggatcgg caagacggcc ctgttgctgg aatgggcgga gcgcaacgcc gtgccgcaca
     2581 cttattgggc ggccgaaaag gagccggccg atcttcagcg ccgcaagctc tatgcccgcc
     2641 tgttaaacct gccgatacgc caggcgccgc ggctggatag ttgggccgag ctatgggatg
     2701 ccgtggcggc aataatcgcc gatcgccgcc aaatcctgat cctggatgag ctgccctatg
     2761 cggtcgaatc cgacccggcc accctgtctg ccttgcaaca tgcctgggat gctcacttcc
     2821 agcactccag cctggtgata gtcctgtgcg gatcgcaggt caaggtgatg gagtccctgc
     2881 aaatgcatca gtcacccctg ttcggccgcc tgacggggca atggtatctt gaaccgctgc
     2941 ccttcagcgc gttgcgcgat tttttccccg actggtcggc cgaggaacgg gtcgccgcct
     3001 acgccgtcac aggcggtgtt ccggcttatc tcaactggct tgatccggcg ctgaatctga
     3061 cggccaatat ccggcgaatc atcctctcgg aaggcagcat gttcctcgct gaaccggcct
     3121 ttttgctcta tgatgaggta cgtgaaccgc aatcctacct ggccacgctc aaagccatcg
     3181 gcttgggcag ccacaccctc ggtgacatca gcaacgacgc gctgattgcc accagtcacc
     3241 tctccgtcta ccttcagcga ctgcaggatt tgaagctggt ggagcggcgg ttgccggcgg
     3301 ctaccccacc ggccaaacgg cgtacctcac ggcttggccg ttatcatctg gccgaccctt
     3361 attttcgctt ctacttccgc tttatcgcgc cgttccacga atacctgccg ttcgacaccg
     3421 aacaggtgct ggagcgtatt cgccacgatc tgcgcggctt cgtcggcgga acggcgttcg
     3481 aggacctggc ccggcagtgg gtcacacaac aaggcaaggc cggaacgctg ccgctgcggc
     3541 cgggaatcgt cggcgcgcac tggagccgga aggtgcagat cgacgtcgtc gccgtggatt
     3601 gggaggcgcg tcaattgctg ctgggcgagt gcaagtgggg cgacgggaac gtcgatcgaa
     3661 gtgtcgtgcg cgatctgatc gagggcaagg gggagttgct gcgcaaggaa ctacccgacg
     3721 gcgaggcgtg gcggttccac tatgccttgt tcacccgagc ggggttgacg ccggcggccg
     3781 cggccgagtt ggcaaagtat gatgggcttc acgttgacct ggcgcgactg gatggggaat
     3841 tagtctgatg cggtcggctc tcgccccgca ctcttctgtc gcatattctc gaaaagatcc
     3901 ggccccaatt ccagcgcggc tacgtcgacc tgctcgcgaa accggcccag cgccgacgag
     3961 aacggttcgg cgaggacagt cgcctccgcc tcgcggatgg gggcggttag attgtcgcgg
     4021 gcttcggcga ttggatagct gtggatcggt ttcatcagga ttagtttccg ttcatgagga
     4081 tatgggtgac caacgcataa tccgagaact cagttatttt gtgataaatg cagcaaaaat
     4141 ccccaagttt tgccgctcat ccatacactc tccacaactc attggtcagc gacgtctgtt
     4201 cgatgtccca ggcctcatcc aggtagcggg agcgatgtac ctgaaacgac gcctcgatta
     4261 cctgcgtcac accatcggcc gaccacgtga ttcggctgaa gacttggccg ttgtcgccgg
     4321 tgatttgcgt ttcgagtgcc cggcaagtga cgtcctcaac gacaggcata cgctcccata
     4381 aagcttccag ttcggccacc gtcaaaagct tgcccaacgt atcggcccac aattgatcgt
     4441 cgtcgacgca atcctgagcg tattccagat cgtggcgaaa gatagcgtca acaaactgct
     4501 caacccacgt ctcgacgttg tattcgtcgg tcgcgtagat gaaatttatc cacgtatcag
     4561 ggaggcgaat gtcgtctatc tcctcatcga gcagctcctc ggttagataa cgatagaccg
     4621 tctcatagtc cacgacgccc agaaaatcga cggcaatata gtgcgccgcc agcaggccca
     4681 tcagagaatc caccgccgct accagttcat cggaactgag cgcgtcaatg ggcggcggag
     4741 ccggatcgcc gatgcgggca ctcacggtaa tgcgcggcgc attctcgaac tggcgctcga
     4801 attcgaggat atagtcgagc cactccgatt ccacctccgg cgacaggtcg ggcgaccgat
     4861 gctcgtgaag catgccgaat tgctcacgca gaatgtctct cttggaatca ttcagcgcgg
     4921 cctggatctg ttgtcgaatt ttatcgatcc attggttgtc aggtgtcatt tgcatggctg
     4981 tcgctatctc cttataaaag tatatcgtga tcgatgacgt cgatgctatc cgaatgaaag
     5041 cagggggtga cgcgtcatcc ggatgtaccc ggatgagagc gtggctttac aactttcaat
     5101 ctacactaaa atagtgtaca ctaccctcat gctcaaaaac atcaccctct cggccgacga
     5161 ggcccttatc cgcgaggcgc ggcggcaggc ggcgctgcag aacacgacgc tcaacgcgct
     5221 gttccgcgaa tggctggagc aatatacaag cgagcaagac gccgacgcgc gccgtttggc
     5281 ggctgcgcgc tatgatgaat ggatgcagca actgtcccac gtcaatgccg ggcgaaagtt
     5341 tacccgcgag gaaatgaatg agcgacgctg aggatacctt ctttctggat accaacatcc
     5401 tcatctacgc ggttgatgaa gccgatctcg tcaaacaaca gatcgccgtg gcgttggtgc
     5461 gtgaggccat tttgagcgga cgggggatca tcagcatcca ggtcgtccag gaattcctga
     5521 atgtcgcgcg gcgcaagttc aagcagccga tgaccatcga gcagtgtcgc ggccacatac
     5581 gaaatgtttt ggaaccgctc tgcgcctatt ttccgtcgat ttcaacgatg gatcgcgctc
     5641 tgctcaccat cgatgaaaca agttactctt tctatgacgc cctgatcctc accgccgcca
     5701 tcgaaagcgg ttgccgcacc ctgtatagcg aagacttgca acatggccgc aagattcagg
     5761 gcttgactat tctgaacccc ttcattggca cggagtaaca gccaggagtt atggaagcct
     5821 ctcatgggga agttcatcgg catcatcgcc gacacgctgg aggacttcca cgagatgccg
     5881 gccggcggcg ggggcggcta ggccgacgct ctgcggccca acccaacgtc gcgtcactgc
     5941 cccaatattg agttgatgct atactcaggg gtacacaaca tgagttggat gagcacaatg
     6001 atcctctgga agaggcgtca tgctctccct gctcaatcgg tgaggagcca aatgaaaata
     6061 gtgactgatt ccgcggccga tttgacctcg gaggatataa gcacgttcga tatttccgta
     6121 gcccctttat atattcagtt ccctgatggc gaggttcgat ccggggatct gagccacgat
     6181 gagttttaca atcgcctgga ggccgtgcgc ccacaattgc cgacaaccgg gcagccttcg
     6241 ccgggcaatt tttcagacct ctatcacccc attcaggaac aaggcgatgg aatcctctct
     6301 atccatattt cttcagggtt aagtggtact gtgaatgccg cccgcaatgg tgcggcgcag
     6361 gaaaatgagg atagcatcca cgtcatcgat agtctgaccc tgtccggcgg attgcgtttt
     6421 caggttcttg ccgcagctca agccatcgcg gcgggttggg atgttcctaa tattcgcgca
     6481 caactggata gagtcagaac ggcgactgaa gttgtctata cgctggatac gctggagtat
     6541 ctggctcgag gtggtcgcat cggccgagtt caagcgttgg ccagttcgat cctcaatatc
     6601 aagccgataa tcaacgtatc gaaatcagat ggtaaatata atactgtcgg caagagccgc
     6661 accatgtcgc gtgccataaa tgacctgaca gagcatttgg cgtctctcta cggcgggaag
     6721 gaacccttgt gggtgtccgt catgcatggc catttcgagg aaaaagcacg gttgttggct
     6781 gaatcgcttc agaggatact ccctgttgag aagctggaag tattacggat ttcgccggtg
     6841 ctcggcgtgc ataccggccc tggaatcgtt ggcgcggccg tggtgccgat gcgctttatg
     6901 aaagagatac actagcctga tgtgggacaa cgctactggt gtggcagctt atgtcaatta
     6961 gccctgatga gagccgatta gagccggtca tacagggcct ccagcgtcct ggataattcc
     7021 ggccagtcga accgggccgt tatatcttcg cgggcggcca ctccccgcga catccgcagt
     7081 tctcgatctg tcaataatat tgtggcggca ttcaccaatt ctgctacatc ccccgacgcg
     7141 catagtagcc cagtgcggcc gtcgcgcacg tattccgcca cctgccccac cgtttcggcg
     7201 actaccggca cgccggcggc caacatgtcg gccagcttga ccgggcattt ggtgcggttg
     7261 agcagggtgt cgtccatcag gtagaggccc acatcggccg cggccagcgt ggccggcagt
     7321 tgctccagcg ccacccagcc cgcgtcgacg acggcgtcgg tcagcccgnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ggtcaggccc cagcgctcct ggcgggcgaa
     7501 gaagcgcttt tgcagcggcg aatagggggc gatgtcgttc cagcctcccg cgccctccca
     7561 atcgtcggtg tccatgacca gccggatgcg gccgcgatga aagcgccaca gccattcggc
     7621 gaccaggccg ctgtaggcct tgggcttgaa ggtgtggacg acgtcgggcc gccatgccag
     7681 cgtctcgcgt accagacgac gggtgatgcc gagcgtgccg cctttcagcg acacgtagcg
     7741 cagttcgata ccatcttcct gccattcgcg atcggcctca tccggcgtgt cccagggcgg
     7801 catgatcagc cgcacggtat ggccgcgatc gaccaacggc cgcgccaatc ccagcgcccg
     7861 actgcgcatg gtttgcttgg gatgcagccc gaacgggccg atcatggcga ttcgcatggg
     7921 gagaatgata cgctattcgg ggggataaag ggagatcgcc ggctgtggga ggaaattacg
     7981 aattacgaat tacgaatgaa gagggaggtc ctccggaaac gggctgccgt tttcgcgcag
     8041 cagttcaagc aggcgcgggt gatcggcgta gacgccgcgg tggtcgggcg gcagcagggc
     8101 ggcgatgcga cacatgaggt cgtcggtctg tgcctggagc cattcgtcgc gtccggcgcg
     8161 gggcatgggc ggcagggtgt agggttcgcc gattcgcgcg gtgatatcaa gcctcttgaa
     8221 ccgcttgagg cggtatttga cgacggcgtc ctcggtgccg acgataccga tagggatgag
     8281 cggcgcgccg gattttgccg ccagatagac ggcgccgggc ttgccgggca gaagggtcgc
     8341 cgtggggctg cgcgtgccct cgggggcaac ggcgagcata tcacccgctt cgaggcgctt
     8401 cagcacggcc cgcagcgcgg cgaggtcggc gtcgaagcgg ttgacccaga tggcatcgac
     8461 cttgcgggcg acgtagcgcc agaaggcgta ttgtttgtac ttttcggcca ggatcatgac
     8521 gatatcgtcc cgctcggcga gcagcatgac cagaaaggcc tccaggcggc cgacgtgatt
     8581 ggcggcgatg atgtaggcgc cggtctgata atcgacgttg ccgatgatct cgatgcgagc
     8641 gatgaccgcc aggacgacgc gggcgatggc acggacgatg cgcaggaaag tgtgtttact
     8701 catctgaatg ggaattgtag cctaactgtg acggagtggt gcaatgctca gacaggttcc
     8761 ttgaaatcgt agaggccgtc ttcgacgcgc cagcgacggc cgcaagcggg attggcgcag
     8821 acgagcagat cgtccacagg ttcgccgagc ggcgtctggc aggcggggca ggcgaagaag
     8881 gcgttctcgt tggcggccgt tgtggagccg gtcaattggc tgagaacaaa gacgctgggc
     8941 gtcagctgcc accacgcccc cgtgccctga atggcgcggt cgagggcgac gagccagccg
     9001 gtgggcacaa tgcgcttgat cggcgcgaag cgaaaatggg aggcggtcag cgtctcgccc
     9061 ggcgcgaagc cgctttcggc caactgttcc cgaatccagg cggggtggaa gtcgaagttg
     9121 agggcgacga actccaccgg ctcgcgctcg tagggcgacc atgtctggcg gccgagaccg
     9181 tagcggagca tggccttgag gttgtgcttg ctggcgaatt cgagaacgta ggcggcctgg
     9241 gggccggcga tgcgagccag ttcgtcgaac agagcgggcg cgtcggcggc gtgatggagc
     9301 gtgcgcacct ggaccagcgt gtcgaacagc ccatccacga acggcatggt gtaccagttg
     9361 ccggccacgt agcggtagcg cggatcatca cccaagtgct gttgagcctc gcgcaacagc
     9421 gaacgagagt aatcgaaaag cacgacgcgg tcgtagccac gatattcatc ggccaggcgg
     9481 ccgaagcccg cgccgatgtc gatgagcgtg cggccgcttg ggggcatcag acgacgcaag
     9541 gcggctcgct cgacctgatc ctcgtaatcg cgcccctgat tgtcccagaa gcgggcgcga
     9601 taatcggagc cttcataatc acaaacggga gtagattcag tcatctcgtg catggtatca
     9661 agaatggcga aggttgccaa ccgggattgt ttcccgggaa acagcgctcg ccatgtttcc
     9721 cgggaaacaa attgaaccgc cattggcatt tcgttgcgga ttcactctat ttcgatagaa
     9781 tcacagtcta aatcaaatga ccgctcgggc ggaaacctga acgactcctc ttcgcggcca
     9841 aaccggggat ttgtgcatgg ccaatgatgt tgccattttt ctcgacctgg acaatttagt
     9901 gattggcgcg cgagacgcca acctgaattt cgacataagt ttgctgatcg atcacatcaa
     9961 gaaacgcacc ggggggcgcg ttgtcttgcg acgggcctat tccggcaacc tgcggcagga
    10021 tcaaaagctg atgaaggagc tggccacggc cggtttcacc atgcaggcgg ccgttccact
    10081 caataacttc ggcaaaaacc tcatcgacat gtacctggtc gttgactcga tggacaccct
    10141 cgtcgacggg caggagtata gtacctacgt gctcgttacc ggcgaccggg attttgtgcc
    10201 gctcattcac gcgctgcgtc gccgcggcaa gcatgtgatt ggcgtcgggc tgaagcacac
    10261 caccagcgag agcctgatgg ggctgtgcga tgagtatgtc tattacgagg atatgctgcc
    10321 cgctccgccc ctgtcggaga gcgaggcgga cgctctgctg actaaagcga tcgatggctt
    10381 gttggccgga gacgagcgtg tgcgcgccag cgtggtcaag gaacgcatga tcgagttgag
    10441 cggggggcaa ttcggccgtt tccagtattc cgacacctcg ttcaccaagt ttctgggtcg
    10501 ccatgcgaac cgcatcgtcc tggagaaaga tggcacgaca acctatgtgc gtcgcgcggt
    10561 tgaactgacg gctccgaccg agctttatcg gcgctatcgc agcgccctga agaagcaaaa
    10621 actgcgcgtc attccgcccc ggttgcggtt ggtgctgttg cgggagatga tcgagatctt
    10681 gcaaaagagc ggcgacacga tggagtggcg cgagctgatc gatcgtctgg cggtgctgca
    10741 tccaggggag gaagagggcg aagcgtcgcg caatatgatc aatgctctga tcgtcctggc
    10801 ccggcgagcg ggcgtcgtgc gcacgctgaa ggggaaaacg ctctctaccg cgccggtgct
    10861 gctggcgctg gaggggccga agctgttcca ggaaagcgta atccgctgcg acacggtcta
    10921 tctggaggcg atccgcgacc tgccggagcc gtttgatgtg gaagaggcgg cgcaggcgat
    10981 ctatgaccag gtcagctacg cgccttactt gaggcaattg ctggcgcgga tggacgcggc
    11041 acaagtcggc taagcgacaa gccccaaaat atagtctata aaaacacaac gagacgctga
    11101 taccagcgtc tcgttgtgtt ttttatcggt ttataccggg tagattggtg tcaggataac
    11161 gacagccggg attagccctt gcttttgccg ttggccttct ctttatactc gcgcaccagc
    11221 tcgcgcttca gcaccttgcc tacggttgtc ttgggcagtt cgtcgcggaa ctggatcagg
    11281 cgcggatatt tgtaggccgc cagctcgcct ttgctccatt cgatgatctc cgcctcggtc
    11341 acgctctcga agcccggccg tttgacgacc caggccatga cgttctcgcc acgcttgggg
    11401 tcgggcacgc cggccacgct gacttccatg actgccgggt ggcgtgacaa tacttcctcg
    11461 atctcgcgcg gatagacgtt gtagccgcca caaatgatca ggtccttctt gcggtcctcg
    11521 atatagaaat agccctcttc atccatgcga gcgatatcgc cggtgtagag ccagccgtcg
    11581 cgcagggagc tggccgtttc gttgggcatg ttccagtagc cctgcatgat cgtcgggccg
    11641 cgcatgatca gttcgccgat ggcgccgata ggcagctcgt cgttcccttc aacagggtcg
    11701 acgatgcgcg cctcgacacc gggcagcggc aggccgatgg atccgatcgg attcttgccc
    11761 ttcagcgggt tggcgtgggt ggccacgtgg gattcggtca ggccataacc ctcaacgagc
    11821 gtgccgccgg tcagttcctc gaatttcttc tttgtctcga ccaatagcgg agccgagccg
    11881 gagatgcagg cgcggatgga gcgcagatca tatttgcctg cggccacgtc cttgttgttg
    11941 ttgatgccca cgtacatggc cgggacgccg gggaagtagg ccggcttgta tttgttgatc
    12001 gactccagca cgtaggggag atcgcgcggg ttggggatga tgagcaggcg gccgccgatg
    12061 gcgacggtga agatgaccgc cgtcaccatg ccgtaggaat ggaagaacgg gatggcgccc
    12121 aacagggtgt cggcgccata tttccagtcg tcgatccagc tggataccat gagggcgttg
    12181 gccaccaggt tacgatgcag gccgatggcc cccttcgaga ggcccgtggt gccgccggta
    12241 tattgcagca gggcgatgtc gtcgccggtg acggtgacgt tcggcttggg gctgcgcgcg
    12301 cccagggcca tgaaatcctt catggtcata tcgcccggct caacgtcgac ccgatcgccc
    12361 tctttcttct ctttgacaag gccgtagagc aagctcttga tgcccggcag ataatccttg
    12421 atgtaggcga cgatgatgcg tttcacattc gtcccgccgc tcttctgcac cttcttgagc
    12481 aatggataat agcgactgag gacaaagacc gtctcggccc cgcagtcctt gagttggtgt
    12541 tgcaactcgc gttcggtgta gagagggttg gtggcgatga cgataccgcc ggccttcagg
    12601 atgccatagt agatgatgac gaattgcggc agattgggca tgtagatgac ggcgcgatcg
    12661 cctttcttga aaccgttggc cgccagacct gccgcgatgg cgtcggacgc cttgttcagc
    12721 tcgtcgtagg tcagttcctt gccctggaag accagcgccg tgttcgtggg gacgcgacgg
    12781 gcggcttctt ccaggaaatg ctgaacggga tacgcgggga cgtcaatcga ctccggcacg
    12841 cctttatcgt aactctttgt ccagggcttg tcggcgtatg tcaggctcat gaggctctct
    12901 ccttatgtaa actataatag cggccggcca ccggtggaca agccacacac gacaggcccg
    12961 atgacacggg ttaattaaag cgaagcagcg cccggcgtgg ccgacttgcc ccaactctcc
    13021 ggcatagcaa gcacaatcgc gcagggggga cgctccactg cttactataa taacgggcat
    13081 atttaaaaat gactagttgc atttggtatg tg
//
LOCUS       DAOMQG010000057        13054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2049_length_13054_cov_10.331795, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000057 DAOMQG010000000
VERSION     DAOMQG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_13054_cov_10.331795"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3116)
                     /gene="mfd"
                     /locus_tag="PLC06_05155"
     CDS             complement(<1..3116)
                     /gene="mfd"
                     /locus_tag="PLC06_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRO89865.1"
                     /translation="MNVSGLLDLLDQIPAFGQLSRALSSEGGAGDLLPLELVRGTRPP
                     VVAQLYRRVATPRQAPIFLLTAKVDSANLWHQALETWLPEGSKLLRLPEPTPLPYDRG
                     PWSDRTRQGRLAVLSSLIPGQHPLIPSVTPPPLVVSSIRAFLQKTLPKRRFIAATRVL
                     RVGQVLDLEKSLTGWVDIGYEPVSVVEAAGQFSRRGGIIDIFPAAAEMPIRIELFGDE
                     IETMRYFDPTTQRSLGPAEGRPAPEGVIITPSREVLPVAARDFVEVWADEPLPVENSL
                     PSWRDDLDSLRAGQAFANLEYYLPLVYPRPVTLLDYLPQDALVVADDWNELKTAADEL
                     IAHAGQIAAEQSTMPPGYQSPLFTWDEIESELAWWQPLVLGEGRPTGPQEAEEPVYIE
                     LGEAFDPGPRYGGQTRPLLNQLKQARREGERVVVFSRQAPRLAELWGEDGGTLIRTLE
                     PLTQAQPDQSDTILPVVTAIDAGTGRTGVAPLETLPDIPPDGSITFVQGSLAEGFILI
                     RQEDHHVLLHLLTDAEIFGWSRPAPRRRHKARPAAPETFFADIKKGDHVVHLDHGIGL
                     FEGLVVRSLGGMEREYLLVKYANGDTLYVPVHHADRLGKWIGPDDADPTLNRLGERTW
                     TQAKAAAQRAADELAQELLDLYATRETIPGHAFPGDTDWQAELEAAFPYHETEDQLRV
                     ISEVKDDMERANPMDRLICGDVGYGKTEVALRAAFKAVMDGKQVAMLVPTTILAQQHY
                     TTFRDRLAPFPVTVEMLSRFRTPAQQRNIVHKLREGNIDIIIGTHRLLSDDVSFKDLG
                     LVIIDEEQRFGVADKERLKQWRTEVDVLTLTATPIPRTLHMALTGVRDISIIDTAPAE
                     RLPVETYVGEADDSRIRTAILRELDRGGQVFFVHNRVQSIHIVRKRLENLVPEARIVV
                     AHGQMSERQLEKIMIDFAEGNVDILLSTTIIESGLDFPNANTLIVDRAEQFGLSQLYQ
                     LRGRVGRSTRRAYAYFFHVPWRTLTPDAQARLDAIAEHTQLGSGYYIAVRDMEIRGAG
                     DFLGGEQSG"
     gene            complement(3123..3734)
                     /gene="pth"
                     /locus_tag="PLC06_05160"
     CDS             complement(3123..3734)
                     /gene="pth"
                     /locus_tag="PLC06_05160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRO89866.1"
                     /translation="MDDGIQPGGERYLIVGLGNPGRAHVFNRHNIGFMAIDRLAARHN
                     IEMKRVQNKAIIGNGRVAGRSVILVKPQTFMNLSGDSVGPLANFYKVPPENILAVYDE
                     LDIPFGALRLREKGGAGGHNGMRSLIQHLGNEFSRMRLGIGRPPGRMEAAAYVLQDFG
                     RDELPIVSEMLSAAVEAIECFVRDGIDLTMSRFNGPVDTQTGK"
     gene            complement(3744..5699)
                     /locus_tag="PLC06_05165"
     CDS             complement(3744..5699)
                     /locus_tag="PLC06_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89867.1"
                     /translation="MTRLLLLFMLLGGSLIACRQDVGPTQPAATNQPETISESTPELP
                     ENTPVETPNATATAEPTATLTPTPIPPKDLVVCVGAEPTDIYLYGDQSPAAMAIRQAI
                     YEPPYSTLNYDYQPVALDKIPTPGDGDAVIETVEVSEGTVIYNANEEIGPLTKGMTVI
                     NANGERVTFSGEPIRMNQMTVDFTFKPLVWSDGTPVTAEDSVFSFHVIGDPNTQLLDK
                     RVRLTKSYKATGERSVRWVGIPGYMEPEYVTHVWTPLPSHQLADFTALEMATLDETTR
                     QPLSYGAFMVESWEEGESVRLVPNPNYYRSAEGLPYLTSLTYKFLSGNNKTLPDGFEG
                     CNIITDDVLSFDAVGAVDDAEAAGTLVEHVATAGVVEQIIFGVDPAAAYESANEVWFQ
                     DARVRQAFTQCTDRQAIVDELTFGRAAVMDTFVPNDHVLHPNDVAQWEYDPAAANALL
                     DEVGYRDTDGDGVRSDISSTAPFSITIGTNIDSDLRLQIIEMVADDLAECGIQANPRA
                     LEAGTWFAPGPGGKVFGRQFDMAQFAWLSRIQPDCGLYLTQNIPGPMIDGFNGWQGVN
                     VSGWSNEAYDAACNEALAMLPGMPGFVEAHQEAMRIFAQELPAMPLFTRMRLAATTPD
                     VLNFQLDPTQTPSLWNAFELDMKLGGS"
     gene            6030..7994
                     /locus_tag="PLC06_05170"
     CDS             6030..7994
                     /locus_tag="PLC06_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRO89868.1"
                     /translation="MTKTSVRTLFVTLFVFSVLLTACGGGANKGETAGSVPFVGGTQA
                     ATPSPTGTAVAPTATGGPTEFDANGIPVGFTEEGRPFRGDLNAPILMETFSDFQCPYC
                     SRFTNETMPGLLENQIANGEVVMVFFDFPLSTIHPQAAAAANAARCAGEGGAAAYWAM
                     HDRLFTNIGEWSNGSANDVFVRYAEELGVAHADFANCLAEKRYAEAIDADFSLGQTRG
                     VNSTPSFFLNDQPVIGAYPLDYFNQAIAAIAAGNTVAAQPETAPREPAVKPTPATIVM
                     DNIGSEMGDPNAPVTIVEYTDYQCPYCVRHATETLPRIISELVDTGRVRYIVKDFPLD
                     NIHPEARSAAVAARCAGEQNAYWEMHDELFARQQEWDGLGAAAVDVFTMIAKGLSLDS
                     DAFAKCMTSGRHDDVIQANQDEGVSLGVRGTPGFFINGFPISGAQPYELFEYAVSLAE
                     EGTLADAYVSQPQEAAQPTPRGPVEVSTDGAYSIGDPDAPVTIVEFTDFQCPFCSRHF
                     LQTFPQIVAEYVDTGKVRYVFMDFPLSSIHPQAQLASEAARCAGDQAAYAGMHDLLFG
                     RQDAWNGRDDAATIFSGFARELGLDTAAFDACLEARTHKDAVLADLDYGTTLGVDGTP
                     AFFVNGHFISGAQPFEVFQQAIESLLADAG"
     gene            8150..9076
                     /locus_tag="PLC06_05175"
     CDS             8150..9076
                     /locus_tag="PLC06_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89869.1"
                     /translation="MSRDGKKVVGFLIGREKDLPDTVMKILNERYEGIQAELVKLGGT
                     FIDEPVVYDVIIDRMSHEIPYYHAYVEYAALNGCYIIDDPFVWANDTKFFGGTLMQKL
                     GVRTPRTVVLPNKDVATDTVPDSFRNLSYPMDWQAIIDYIGSPAIFKDVRSGGRRFAH
                     RVANVGELIQRYDESGTRTMILQQVIETNKQYHCVVIGREKTILMPFSMETNHYLGVD
                     SHLEKNQAEKMTETALKIADVYGYDINMTEFALLGEELYLINATNPSPLVSRDLMTET
                     QFDWLCNEIADLAARRAANPPTMKFPVKYSID"
     gene            9242..11386
                     /gene="glgP"
                     /locus_tag="PLC06_05180"
     CDS             9242..11386
                     /gene="glgP"
                     /locus_tag="PLC06_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRO89870.1"
                     /translation="MNELIEKMPEKLRRLPELAYNVWWSWNAEAREIFRRLDYPLWRR
                     TQHNPVRVLRLVSPQTLAARAGDASFLRRYNKVLMDFDRAMENGHSWFHVTYPNLTRK
                     TIAYFSFEFGLHSSLPIYSGGLGILAGDHAKEASDLGLPFVGIGFLYPQGYFRQHVPS
                     HGWQEAIYEPIDMRDVPILPVRDAHGGELRISVEMESRRVHARIWRVQVGRVPLFLMD
                     TDVPENEPWDRELSARLYSGDSEMRIRQEIMLGIGGVRLLRVLGYDPAVLHMNEGHSA
                     FLLLESIREYVEGGMDFAGASELVRSKSIFTTHTPVPAGHDAFSFHLIDKYFQGYWGK
                     LGIGREAFLNLGHHREPWGDAFNMTVLALHLGGQFNGVSKLHGEVSRDMWKHVWPSLP
                     VDEVPITSITNGIHVPGWTAGEMRHIFNKYLGADWEEHHDDPVLWERLADVPDEELWR
                     VHTALKAKLMTYLRARARRSWVNGKCDPTEVLVGGTLLDPDALTIGFARRFATYKRAS
                     LLFTDMDRLQQILLNMHRPVQIIFAGKAHPADEPGKHLIQQVYTMAKASQFGGRLAFV
                     EDYDMHSARYFKQGVDVWLNTPRRPREASGTSGMKASLNGIPNLSILDGWWVEGYNGA
                     NGWAIDDRQYAGAEEQDYQDAQRIYRLLEEEIVPLYYNQDRDGIPRGWVEVMREAIRS
                     NAWRFSTRRMVKEYTRELYLNAMLGVKVGEPA"
     assembly_gap    11506..11605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11722..13017
                     /locus_tag="PLC06_05185"
     CDS             11722..13017
                     /locus_tag="PLC06_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO89871.1"
                     /translation="MAQDGLRRFTYSKTFIVGFGFLGISIIWPIFNQYIPLFLQAGNP
                     QFEAQLLAEGRAIPDIAGFGLGPSLALFIMTWDNLINIFLQPWVGARSDRTWNRFGRR
                     KPWILLGLPIAAIGFILLPFAPTALALAAFILITNVGMALFRSPTVAWLGDLFPPDDR
                     SKANGIINLMGGIGSLIAFLGGGYLFNNFGRAAPFIAGAILMVVALLVAVWRVREPRH
                     LVEGDDTEPVRSVMENLRLVAGRADRGGLYVLLGILLWFIAFNGLEAALSSFAVFSLG
                     IAPGSASIYSASISLSFIVFALPAGLLGTSLGRRKVIMVGLIGLAVLFGVAFFLIQGA
                     ATFVVVLVVAGLFWAMVNVNSLPLVYDFGDERQIGAFTGLYYFSSQLAAVLGPTLGGI
                     VVDVMGDQYRWMFPFSAIFMALALVVILRIRPTRPAVTA"
BASE COUNT         2424 a         3760 c         3979 g         2791 t
ORIGIN      
        1 ccgctttgct cgccgccgag gaagtcgccc gcgccgcgaa tttccatgtc gcgcacggcg
       61 atgtagtagc ctgaaccgag ttgggtatgc tcggcgattg catcgaggcg agcctgtgcg
      121 tccggcgtca atgttcgcca ggggacgtgg aagaaatagg cgtaggctcg ccgcgtgctg
      181 cgcccgacgc ggccgcgcaa ctggtagagc tgcgacaggc cgaactgctc cgcgcgatcg
      241 acgatgaggg tgttggcgtt ggggaaatcc agcccgcttt cgatgatggt cgtgctgagc
      301 aggatgtcga cattgccctc ggcaaagtcg atcatgatct tttccagctg tcgctcgctc
      361 atctggccgt gggcgacgac gatccgcgcc tcgggcacca ggttctccaa tcgcttgcgg
      421 acaatgtgga tgctctgcac tcgattatgg acgaagaaga cctgaccgcc gcgatccagt
      481 tcgcgcagga tagcggtgcg gatgcgcgaa tcatcggcct cgccgacgta ggtctcgacg
      541 ggcagtcgct cggccggggc ggtgtcgatg atgctgatgt cgcgcacgcc ggtcagggcc
      601 atgtgcaggg tgcgcgggat gggcgtggcc gtcagagtca gcacgtcgac ttcggtgcgc
      661 cactgcttga gcctttcttt atcggcgacg ccaaagcgct gctcctcatc aatgatgaca
      721 agccccaggt ccttgaagga gacgtcatcg gacagcaacc ggtgggtgcc gatgatgata
      781 tcgatgttcc cctcgcgcag cttgtggacg atgttgcgtt gctgggccgg tgtgcggaag
      841 cgtgacagca tctcgaccgt caccgggaag ggggccagcc gatcgcgaaa tgtggtgtag
      901 tgttgctggg ccaggatggt ggttggcacg agcatagcca cctgcttgcc gtccatgacc
      961 gccttgaagg ccgcgcgcag cgctacctcg gtcttgccgt agcccacatc accgcaaatg
     1021 aggcggtcca ttgggttggc ccgctccatg tcgtccttga cttcgctgat gacgcggagt
     1081 tggtcctccg tctcgtgata ggggaaggcg gcctcaagct cggcctgcca atcggtgtcg
     1141 cccggaaaag cgtggccggg gatggtctcg cgggtggcgt acaggtcgag caattcctgg
     1201 gccaactcat cggccgcgcg ttgggcggcc gccttggctt gcgtccaggt gcgctctccc
     1261 agtcgattca gcgtcgggtc ggcgtcatcg ggaccaatcc acttgcccaa ccgatcggcg
     1321 tgatgaaccg gcacgtagag cgtgtccccg ttggcgtact tcaccagcag atactcgcgc
     1381 tccatgcccc caagactgcg cacgaccaac ccctcgaaca ggccgatacc gtgatcgaga
     1441 tggacgacgt ggtcgccctt cttgatgtcg gcgaagaatg tttccggcgc ggcggggcgg
     1501 gccttgtgcc ggcgacgcgg cgcggggcgg ctccagccga atatctcggc gtcggtcagc
     1561 agatgcaaga ggacgtgatg gtcttcctga cgaatgagga tgaatccctc ggccagactt
     1621 ccctgaacaa aagtgatgct gccgtccggc ggtatgtcgg gcagcgtctc cagcggcgca
     1681 acacccgttc gccctgtccc ggcgtcaatg gccgtcacga ccgggaggat ggtatccgat
     1741 tgatcaggct gggcctgggt gagcggttcc agcgtgcgga tgagcgtgcc gccgtcctcg
     1801 ccccatagtt cggccagccg tggggcctga cggctgaaca caaccacgcg ctcgccctca
     1861 cgccgcgctt gtttgagctg gttcagcaac ggccgcgtct gtccgccata gcgagggccg
     1921 ggatcaaacg cttcgcccaa ttcaatataa accggctctt cggcttcctg tgggccggtc
     1981 ggccgtccct cgcccaacac gagaggctgc caccaggcta actcggactc gatctcatcc
     2041 cacgtgaaga gcgggctttg atagcccggg ggcatggtgg attgttcggc ggcgatctgc
     2101 ccggcgtggg cgatcagttc atcggcggcg gttttcagtt cgttccaatc gtcggcgacg
     2161 accagcgcgt cttgcggcag atagtccagc aaggtaaccg gccgtggata gacgagcggc
     2221 agatagtatt ccaggttggc aaaggcttgt cccgcgcgca ggctgtccag atcgtcgcgc
     2281 catgagggca ggctgttttc caccggaagc ggctcatccg cccacacttc cacaaaatcg
     2341 cgggcggcaa cgggcaagac ttcgcgcgat ggggtgatga tgaccccttc aggcgcgggt
     2401 cgcccttcgg ccgggccaag cgagcgctgc gtggtcgggt cgaagtagcg catcgtctcg
     2461 atctcgtcgc cgaacaactc gatgcgaatg ggcatctcgg ccgcggcggg aaagatgtcg
     2521 atgatgccgc cgcgccggct gaactgaccg gccgcttcaa cgacgctgac cggctcatag
     2581 ccgatatcga cccacccggt gaggctcttc tccaaatcca gcacctggcc cacgcgcaag
     2641 acgcgcgtcg cggcgataaa gcgtcgcttg ggcagggtct tttgcaggaa cgcgcgaatt
     2701 gaactgacta ccaagggcgg gggagtgacg gatgggatca gcgggtgctg ccccgggatg
     2761 aggcttgaca gcacggccaa tcgcccctgg cgggttcggt cgctccaggg gccgcgatcg
     2821 tagggcaatg gggtaggttc gggcaggcgc agcagcttgc tgccttcggg cagccatgtc
     2881 tccagggcct gatgccagag gttggccgaa tcaactttag ccgtcagcag gaagatgggg
     2941 gcctgacgag gcgtagctac gcgccggtag agttgggcca ccaccggcgg ccgggtgcct
     3001 ctgaccagtt ccagcggcaa cagatcgccc gcgccgcctt cggacgacag cgcgcgcgac
     3061 agctgcccga aagcgggaat ctggtcaagc aggtccagca ggccggatac gttcatgaat
     3121 cgttacttcc ccgtctgagt gtccacgggg ccattgaagc gcgacatcgt cagatcgatg
     3181 ccgtcgcgca cgaagcactc aatcgcctcg acggcggccg acaacatctc gctgactatc
     3241 ggcagctcgt cacgcccgaa atcctgcaat acgtaggccg cggcttccat ccggcctggc
     3301 ggccgcccaa tgcccaggcg catgcgcgag aactcgttgc ccaaatgctg gatgagcgaa
     3361 cgcatgccgt tgtggccgcc cgcgccgcct ttctcgcgca gccgaagcgc cccgaacggg
     3421 atatccagct catcgtaaac cgccagaatg ttctccggcg gcaccttgta gaaattagcc
     3481 agcggcccga cggaatcgcc actcaggttc atgaaggtct gcggcttgac caggatcacc
     3541 gaacgaccgg cgacgcggcc gttgccgatg atggccttgt tttgtacgcg cttcatctcg
     3601 atgttgtgcc gcgcggccag ccggtcgatg gccatgaatc cgatattgtg gcgattgaaa
     3661 acgtgcgccc gcccgggatt ccccaggccg acgatcaagt aacgttcgcc gccgggctgt
     3721 atgccgtcat ccatctttgc ttcctatgag ccgcccaact tcatatccaa ttcaaatgcg
     3781 ttccagagag atggcgtttg cgtcgggtca agctgaaaat tcaacacatc cggcgtcgta
     3841 gctgccagac gcatgcgggt gaataagggc atggccggca actcctgggc gaagatccgc
     3901 atggcctcct gatgcgcctc gacgaatccc ggcatgcccg gcaacatggc cagcgcctcg
     3961 ttgcatgccg cgtcataggc ctcgttcgac cagccggaca cattcacccc ttgccacccg
     4021 ttgaacccgt cgatcatcgg gccggggatg ttttgggtca gatagaggcc gcaatccggt
     4081 tgaatccggc tgagccaggc gaactgggcc atgtcaaact gacggccgaa gaccttgcct
     4141 cccgggccgg gggcgaacca tgtgcccgct tccagggcgc gagggttggc ctgaataccg
     4201 cattcggcca gatcatccgc gaccatctcg atgatttgca ggcgtaaatc gctgtcgatg
     4261 ttcgtgccga tcgtgatgga gaagggcgcc gtcgagctga tatcgctgcg gacgccgtcg
     4321 ccatccgtgt cgcgatagcc aacctcgtcg agcaaggcgt tggccgccgc cgggtcatac
     4381 tcccattggg ctacatcatt gggatgcagg acgtggtcgt tggggacaaa tgtgtccatg
     4441 acggccgccc ggccaaaggt caattcgtcg acaatcgcct gacgatcggt gcattgggtg
     4501 aatgcctgtc gcacgcgcgc atcctggaac cagacttcgt tggcggattc ataggctgcc
     4561 gccggatcga cgccgaagat gatctgctcc acgacgccgg ccgtggccac atgttcaacg
     4621 agcgtgccgg ccgcctcggc atcatcgaca gcgccaacgg cgtcaaagga tagcacgtcg
     4681 tcggtaatga tattgcatcc ctcgaagccg tcgggcagcg ttttgttgtt gccggacagg
     4741 aacttgtagg tcagtgaagt caggtagggc aagccctcgg ccgaccgata gtaattgggg
     4801 ttgggaacca ggcgaacaga ttctccttct tcccacgatt cgaccataaa cgcgccataa
     4861 ctcagaggct ggcgggtcgt ttcgtcgagg gttgccattt ccagcgcggt gaagtccgcc
     4921 aactggtgac tgggcaaagg cgtccagaca tgggtcacgt attccggctc catgtagccc
     4981 ggaatgccga cccagcgcac ggatcgctcg cctgtcgctt tgtacgactt ggtcaggcgg
     5041 acccgtttat cgaggagctg ggtattcgga tcgcctatga cgtggaagct gaagacggag
     5101 tcctcggccg tcaccggagt tccgtctgac cacaccagcg gcttgaaggt gaaatcgacg
     5161 gtcatctggt tcatcctgat cggctcgccg gaaaacgtga cgcgctcgcc gttggcgttg
     5221 atcactgtca tgcccttcgt gagggggcct atctcttcat tggcgttata gatgaccgtg
     5281 ccttcggaca cctcaaccgt ttcaatgacc gcatccccgt cgccgggggt cggaattttg
     5341 tccagtgcca ccggctgata atcgtagttg agtgtcgaat agggaggctc gtagatggct
     5401 tgacgaatgg ccattgccgc cggagattga tcgccataga ggtaaatgtc ggtcggctcc
     5461 gcacccacgc acacgacgag gtccttgggc ggaatcgggg tgggggtgag cgttgccgtt
     5521 ggctcggccg tggccgttgc gttgggcgtt tccacgggcg tattctccgg cagttcgggt
     5581 gtgctttcgg atatggtttc gggttgattg gttgctgccg gttgggttgg cccaacatct
     5641 tgccggcaag ctatgagaga gccgccaagc agcatgaata acaatagtag tcttgtcacg
     5701 aattgtaatc ctctcggaaa gtgacttttg aaaagacggt tcgatgaagt caaaagctca
     5761 ataggttagt ctagcatatt tcatgatgca tgacgaccgg acggttttct taacggtcca
     5821 tgctattgtt tccctaatgg attcccgcga gagtaattgg cggtatactt ggtctattcg
     5881 cgtctcgtcc atcgtcggac gtgcaggaat taagagatca accactcgtt gccggtaagc
     5941 ttttgggctg ccggcttgtc tatttaagaa ataccctaat gcgtctatgg gcatcgttgc
     6001 ctgtcggggc gcaaattgtg aatccggtaa tgaccaaaac ttcagttcgt acgctattcg
     6061 ttacgctgtt tgtgttttct gttcttttga ccgcatgtgg cgggggcgcc aataagggcg
     6121 agaccgccgg ctcagtcccg ttcgtggggg gcacgcaagc tgcgacgcca tcgcctaccg
     6181 ggaccgccgt cgctccgacg gccacggggg gaccgaccga atttgacgcg aacggcattc
     6241 ccgtcggctt tacagaggaa ggccggccct tccgcggcga ccttaatgcg ccgatcctca
     6301 tggagacctt ttcggatttc cagtgcccct actgcagccg cttcaccaat gagaccatgc
     6361 ccggcctgtt ggagaatcaa attgccaacg gtgaagtcgt gatggtcttc ttcgatttcc
     6421 cgctcagcac cattcacccg caggcggccg cggcggccaa cgctgcccgg tgtgcgggcg
     6481 agggcggcgc ggccgcctat tgggccatgc acgatcggtt gttcaccaat atcggcgagt
     6541 ggtcgaatgg gagcgccaac gacgtcttcg tgcgctatgc cgaggagttg ggggtggctc
     6601 acgctgattt tgcgaactgc ctggccgaga agcgatatgc cgaggcgatc gatgcggatt
     6661 ttagtctggg ccaaacgcgc ggcgtcaaca gcacccccag cttcttcctg aacgatcagc
     6721 ctgttatcgg agcctatccg ctggattatt tcaatcaggc tattgccgcc atcgccgccg
     6781 gcaatacggt ggcggcacag ccggagaccg ctccgcgaga gcctgccgtc aagccgacgc
     6841 ctgcgactat cgtgatggac aatatcggca gcgagatggg cgaccccaac gcgccggtca
     6901 cgatcgttga gtataccgat tatcaatgcc cctactgcgt gcgtcacgcc accgagacgt
     6961 tgccgcgcat catctcggag ctggtggata cggggcgcgt tcgctatatc gtcaaggact
     7021 tccctctgga caacatccac cccgaggcac gctccgcggc cgtggcggct cgctgcgcgg
     7081 gcgagcagaa tgcctactgg gagatgcacg acgaactttt cgcccgccag caggaatggg
     7141 atgggttggg cgcggccgcc gtcgatgtct tcacgatgat tgccaagggc ctatcgctcg
     7201 acagtgacgc gttcgccaag tgcatgacca gcggtcggca tgacgacgtg attcaggcca
     7261 atcaggacga aggggtatcg ctgggcgtgc gcggcacgcc ggggttcttc atcaacggct
     7321 tccccatcag tggcgcgcag ccctacgaat tgtttgagta tgccgtatcc ctggccgaag
     7381 aaggaactct ggctgatgcc tacgtttcgc agccccaaga ggccgcccaa cccactccca
     7441 gggggccggt cgaggtcagc accgacggag cgtatagcat cggcgatccc gacgcgcccg
     7501 ttaccatcgt cgaattcacc gatttccagt gccccttctg cagccgccat ttcttgcaga
     7561 ctttcccgca aatcgtggct gagtatgttg acacgggcaa ggtgcgctat gtgttcatgg
     7621 atttcccgct gagcagcatt cacccgcagg cgcagttggc ttcggaagcg gcgcgctgcg
     7681 cgggcgatca ggctgcctat gcggggatgc atgatctgct gtttgggcga caggatgcct
     7741 ggaacggccg cgatgatgcg gcgaccatct tttccggttt tgccagggag ctggggctgg
     7801 atacggccgc gttcgacgcc tgcctggaag cccgaaccca caaggatgcg gttttggccg
     7861 atctggacta tggcacgacc ctcggcgtcg atggtacgcc ggcgtttttc gtgaacggcc
     7921 atttcatctc gggagcgcaa ccctttgagg tcttccagca ggcgattgaa tcattgctgg
     7981 ccgacgcagg ttaggccgca aaattgggga catcgcccaa tttgggtatc ttgccggaat
     8041 tggatatagt cattgtcagg gagtgttctc cgagccacaa tcactcccgg cgcacggatt
     8101 acagcctgaa tagcttcgcc tttccctgaa caggtgcggg agatatagca tgagccgaga
     8161 cggaaagaaa gtagtcggtt ttcttattgg gcgcgagaaa gatttgcccg ataccgtgat
     8221 gaagattctc aatgagcggt atgagggcat ccaggccgag ctggttaagc tggggggtac
     8281 tttcattgac gaacctgtgg tctatgatgt gatcatcgat cgcatgtcgc atgaaatccc
     8341 ttactaccac gcctatgtag agtatgccgc cttgaatgga tgctatatca tcgacgaccc
     8401 cttcgtttgg gccaacgaca ccaaattttt tggcgggacg ctgatgcaaa agctgggcgt
     8461 ccgtaccccg cgcaccgttg tgttgccgaa caaggatgtc gctaccgaca ccgtgcccga
     8521 cagcttccgc aatttgtctt accccatgga ttggcaggcc atcatcgatt acatcggcag
     8581 cccggctatc ttcaaggatg tgcgcagtgg tggccggcga tttgcccatc gtgtggccaa
     8641 tgtgggggaa ttgatccagc gctacgatga gagcggcact cggacgatga tcctgcaaca
     8701 agtgatcgaa acgaataagc aatatcattg cgtggtcatc ggccgcgaga aaacgattct
     8761 catgccgttt tcgatggaga caaatcacta tctcggcgtt gacagtcatc ttgaaaagaa
     8821 tcaggctgaa aagatgaccg agacggccct gaagatcgcc gacgtttatg gttatgacat
     8881 caatatgacg gagtttgcgt tgttgggcga agagctatat ctgatcaacg ctaccaatcc
     8941 gtcgcctctg gtaagtcgtg acttgatgac ggagacccaa ttcgattggc tctgcaatga
     9001 gatagccgat ctggcggcgc ggcgagcggc gaatccaccc accatgaagt ttcccgtcaa
     9061 atacagtatt gattgatcgc gcgatcgata aacttccctg ccccgatttt tcacgtgcgc
     9121 gctcttgacg cacgcattat aattcatgta ttgtggttga ggtatcaacg acctcgaccg
     9181 cttttctgat tgacgaataa tggtcaaaaa ttccttttgc taagaaagga gcaggagtcg
     9241 aatgaacgag ctaatcgaaa aaatgccgga aaagcttcgg cgattgcctg agctggctta
     9301 taacgtttgg tggagctgga acgccgaggc gcgggagata tttcgtcgcc ttgactatcc
     9361 cttgtggcgg cgaacccagc acaatcctgt gcgcgtgttg cggctggtca gcccgcaaac
     9421 gttggccgcc cgtgccgggg atgcctcttt cctgcgccgc tataataaag tgctgatgga
     9481 tttcgatcgg gccatggaaa acggccatag ctggttccac gtgacctatc ctaacctgac
     9541 gcgcaagacg atcgcttact tttcgtttga attcgggctt cacagctcgc tgcccatcta
     9601 ctccggcggc ctgggaattc tggcaggcga ccatgccaaa gaggccagcg acctgggatt
     9661 gccttttgtc ggcatcggct tcctctatcc ccaaggctat ttccgccagc acgtgccgtc
     9721 gcatggctgg caggaagcga tctacgagcc gatcgacatg agagatgtgc ccatcctgcc
     9781 ggttcgtgac gcccatggtg gcgagttgcg aatcagcgtc gagatggaaa gccgccgcgt
     9841 tcatgcccgt atatggcgcg ttcaggtcgg ccgcgtgccg ctcttcctga tggataccga
     9901 cgtgccggag aatgagcctt gggaccgcga gttgtccgcg cggctctatt cgggcgacag
     9961 cgagatgcgc atccgacagg agatcatgct gggcattggt ggcgtccgcc tgctgcgcgt
    10021 gttaggttat gaccctgccg tgctgcacat gaacgaaggc cactccgcat ttctgcttct
    10081 cgaatcgatt cgcgagtatg tggaaggggg gatggatttc gccggcgctt cggaactggt
    10141 acgctccaag tcgatcttca cgacccacac gcctgtgcct gccggtcatg atgctttctc
    10201 ctttcatctg atcgacaaat actttcaggg gtattgggga aagttgggca tcggccgcga
    10261 ggcgttcctg aacctggggc atcatcgtga gccatggggc gatgccttca atatgacggt
    10321 gctggcgctg cacctgggcg gccagttcaa cggcgtcagc aagctccacg gcgaggtgtc
    10381 ccgcgacatg tggaaacatg tatggccaag cctgccggtc gacgaagtgc cgatcacatc
    10441 catcaccaac ggcattcatg tgccgggctg gacggcgggc gagatgcgtc acatcttcaa
    10501 taaatatctg ggtgccgatt gggaagagca tcacgatgat ccggtgctgt gggagcgttt
    10561 ggccgatgtg cctgatgagg agttgtggcg ggtccacacc gctctcaagg ccaaattgat
    10621 gacctatttg cgtgcgcgtg cgcgacgttc atgggtcaac ggcaagtgtg acccgaccga
    10681 agtgctcgtt ggcggcacgc tgctggatcc cgacgcgttg accattggct ttgcccgtcg
    10741 cttcgccacc tataaacgag cgtccctgct gttcaccgac atggatcgct tgcagcagat
    10801 cctgttgaac atgcaccgac cggttcagat catctttgcc ggtaaggccc accccgcgga
    10861 tgaacccggc aagcatctca tccagcaggt ctacaccatg gccaaggcga gccagttcgg
    10921 cggccgcctg gcctttgtcg aagattacga tatgcactcc gcgcgctact tcaaacaagg
    10981 ggtcgatgtg tggctgaaca cgccgcgacg gccgcgggaa gccagcggta ccagcggcat
    11041 gaaggcctcg ctcaacggca ttcccaacct cagcatcctc gatggctggt gggtcgaggg
    11101 atataacggc gcgaacggct gggcaattga tgatcgccag tacgccggcg cggaagagca
    11161 ggattatcaa gacgctcaga ggatctatcg tctgctcgag gaagagattg tgcccctgta
    11221 ctataatcag gatcgcgacg gcattcccag aggctgggtc gaagtgatgc gcgaagcgat
    11281 tcgttccaac gcctggcgct tcagcacccg ccgcatggtg aaagagtaca ccagggagct
    11341 ttacctgaac gcaatgctgg gcgtgaaggt tggcgagccc gcttaatgca gtcccggcga
    11401 acacaaaatt agcaattcgg ttatactacc cggctgaacg actttccgtt cagccgggtt
    11461 ttgcattgtc actatgaacg tctggttgcg tcgcttactg atactnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnngcggc acccttctga actttcgatc ttgtttcctg
    11641 gccaactgtt gatgaatgta ggcggttccg gaaagctcgc gttatacttc ctgtggcgac
    11701 atcatacaac aggaggctgc tgtggctcag gacggattgc ggcgattcac atatagcaag
    11761 acattcattg tcggtttcgg ctttctcggc atcagtatca tctggcccat cttcaatcaa
    11821 tacatcccct tgttcttgca ggcgggtaat ccccaatttg aggcgcaact gctggccgaa
    11881 gggcgggcaa ttcccgacat cgcgggcttt ggcctggggc cgtcgctggc gctgttcatc
    11941 atgacctggg acaacctgat caacattttc ctgcaaccgt gggtgggcgc gcgatccgac
    12001 cgcacgtgga accggttcgg ccgtcgcaag ccatggatcc ttctcggcct tcccatcgcc
    12061 gccattggtt tcatcctgct gcccttcgct cccacggccc tggcgctggc ggctttcatc
    12121 ctgatcacca acgtaggtat ggcgctcttc cgctcgccga cggtagcctg gttgggcgac
    12181 ctcttcccgc cggatgatcg tagcaaggcc aacgggatta ttaacctgat gggcggcatc
    12241 ggcagcctta tcgccttttt gggcggcggc tatttgttca ataatttcgg ccgcgccgcc
    12301 ccgttcatcg ccggcgccat cctgatggtg gtggccttgt tggtggcagt gtggcgcgtt
    12361 cgtgaaccac gacatctggt agagggtgat gacactgagc ctgttcgttc ggtgatggag
    12421 aatttacgcc ttgtggccgg ccgggccgac cgcggcggcc tctatgtcct gttgggcatc
    12481 cttctgtggt tcatcgcctt caacgggctg gaagcggcgc tctcttcgtt tgccgtcttc
    12541 tcgttgggca tcgctccggg atcggcatcg atctactcgg cgtcaattag cctttcgttc
    12601 atcgtattcg ctcttccggc cgggcttttg ggcacgtcgc tcggccgccg gaaggtaatt
    12661 atggtgggac taattgggtt ggccgttttg tttggcgtgg ccttcttctt gatccagggc
    12721 gcagctacgt ttgtcgtcgt tctggtggtg gccggtttgt tctgggccat ggtcaacgtc
    12781 aatagcctgc cgctggttta tgacttcggc gacgagcggc aaatcggcgc cttcaccggc
    12841 ctgtattatt tctcttccca actcgcggcc gtgctcggcc ctacgttggg gggcatcgtc
    12901 gtcgatgtga tgggtgacca gtatcgctgg atgtttccct tcagcgctat cttcatggct
    12961 ctggcgcttg tcgtgatctt gaggattcgc ccgacccgtc cggcggtcac tgcttaaata
    13021 tattgcatta cggcgctgca tcggcagcaa aggc
//
LOCUS       DAOMQG010000058        13005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2060_length_13005_cov_8.665869, whole genome shotgun sequence.
ACCESSION   DAOMQG010000058 DAOMQG010000000
VERSION     DAOMQG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 13005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13005
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2060_length_13005_cov_8.665869"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1032
                     /gene="mraY"
                     /locus_tag="PLC06_05190"
     CDS             46..1032
                     /gene="mraY"
                     /locus_tag="PLC06_05190"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRO89872.1"
                     /translation="MGWALTVAAATFLLSVIWGAPLIEIMRRLKLGKNIRIDGPATHA
                     VKMGTPAMGGVLIIGWTLVVSLVVSIVQIVQALETAESVVIPLGVMVAYSILGGIDDY
                     QGFRLRPGEGMRARVKLWIQLAIALIAAVLLYWLVNDRHGWVAVPTVPLLLDIGLLYI
                     PMAVILITGSANAVNLTDGLDGLAGLISATAFVAYGIIAFLQDQQFLVVFSFIVIGAT
                     FGFLWYNAKPAQMFMGDVGSQALGAALGVVALMTNQWLIFPIIIAIPVATELSSTLQV
                     LYFKATGGKRLFKMAPLHHHFELLGWSETQIVQRWWIIAMLTAMIGIALALV"
     gene            1038..2399
                     /gene="murD"
                     /locus_tag="PLC06_05195"
     CDS             1038..2399
                     /gene="murD"
                     /locus_tag="PLC06_05195"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRO89873.1"
                     /translation="MDALNGKRLVILGMARQGTALARFAVDAGAFVTLSDMRSAERLG
                     AQMELLADLPADRLRYVLGGHPMSLLDECDVLAISGGVSTDMPLVVEATRRGIPLTND
                     SLEFMRRVPALVIGITGSAGKTTTTALTGQMGQKSDRRTWIGGNIGRPLITDLPQISA
                     DDLVVQELSSFQLEIWDHCSPPVAAILNVTPNHLDRHKTMASYAAAKSHILRSQRSDG
                     IAVLSADDPGSLEMREFVRGRLRTFSAHQPVTDGAFLRDGQIWLNDDQGEHWVCPVTA
                     SRLIGPHNILNILAAVALADSAGVPRESIAEGIRLFDGVPHRLEIVRRVNGVQYVNDS
                     IATAPERALAALAAFDEPVILLAGGRDKDMAWGQWGRQVSRRVKHVVLFGELAPLLAR
                     HLTDSVSSTRVDTLAEAVQVAHAEASAGDVVLLSPGGTSFDGYTDFVERGEHFRLLVR
                     ELE"
     gene            2414..3586
                     /locus_tag="PLC06_05200"
     CDS             2414..3586
                     /locus_tag="PLC06_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRO89874.1"
                     /translation="MAAINVIKKRGTSLWAGRNKLAVDYWLLLATGALLVVGFLMVYS
                     TTFDLGDRYEGNPVYFVTRQLTAMGLGVAVVIVLLQFDYHILRRISVPLIVVTIVILL
                     VMLLFGQVDAYGAIRTLSDGSYQPSELAKLAMILYIAHWLDSKGERIKTLNYGLFPFA
                     AITGIVCGLIVLQPALSTAILIGLISFTLFFVAGADLKQVLAAGVVAMVMLFFLMFAL
                     DHASQRINDYMAAFRDPQQAGYQVKQSLAALANGGYWGVGLGKGDQKFGPLPLAHTDG
                     VFAIVGEEMGLLGSIGVVLLFAFMAWRGFLVATRARDTYGFLLAVGVTAWISYQALIN
                     IAVITAVIPFTGMPMPFLSYGGSSMLTTIIGVGILLCVSRDAAMGRQSARRQPKSS"
     gene            3597..4748
                     /locus_tag="PLC06_05205"
     CDS             3597..4748
                     /locus_tag="PLC06_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRO89875.1"
                     /translation="MRVLVCAGGTGGGIYPALAAVTALQAQGVGDDDILWIGTKGEME
                     ETLVPRAGLRLETIPGGPIVGVPLGTRARNAVRLGRGIGVASGHVRRFRPNVMFMTGG
                     YMAAPVALAARRHGVPIVIYLPDVEPGSSIRFAVPMARRIACTTDGSAAFVPQEKMVV
                     TGYPVRPDIRAAKSLTQAEALAKFNLQPGRPTLFVFGGSRGARNINQALMAALPALLT
                     RAQVIHISGTLTWPEVEANAATLPADLQAWYRPYPYLHEEMGAAFRAADLALARAGAS
                     MLGESPAFGLPAILVPLTFAWRYQKVNADYLTERGAAVQLIDETLAETLLPTVQDLLF
                     DSNKLAQMATAATMLDRPDAATDLAQVIRSEAKPEKKPTSTLPQEETSC"
     gene            4742..5335
                     /locus_tag="PLC06_05210"
     CDS             4742..5335
                     /locus_tag="PLC06_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRO89876.1"
                     /translation="MLSLTTAFWGLVVLFAVAGMMRGWTREIVVTASIILALFAINQL
                     MSPIFGLLNWDNNAVQPDNIRRYQFFIMSSFLLLLAFAGYQGPTLASSRVGDRLNRRD
                     NLQDKLLGLSVGAINGWLIIGSLWSFLEYKIIAANTWLRFPPNIGYPFDVSRITRPAP
                     ELAPIIDNLPIPFLTQSPYILLLLLILFVLFLLIVLL"
     gene            5366..6784
                     /gene="murC"
                     /locus_tag="PLC06_05215"
     CDS             5366..6784
                     /gene="murC"
                     /locus_tag="PLC06_05215"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRO89877.1"
                     /translation="MGTQLTPGMHIHIVGIGGAGMSAIARVLLGRGFRVSGSDKQSNA
                     QTAALAAEGVTVYAGHAAEHIDGADLVVISSAIPHANPEWVAAEARGLPVLKRADLLG
                     VLMQGSVGVAVAGSHGKTTTTGMIAHILMECDLDPTVILGGTLAELGGNGRYGEGPHF
                     VVEADEYDYMFLGLRPEVAVITNVEHDHPDLFPTADAYREAFGRFVALLPSGGRLIIC
                     ADDPGAADLLTELEPGNVTVTTYGLGESVTGIVPTVRALDMRPNQMGGSDFVVENGGQ
                     TVGLARIRLPGAHNVRNALAALTVAMNLQIDFSPACRALAGFGGVQRRFQLIGEVGGV
                     TIIDDYAHHPTEIKATLAAARQRFPGRRLWAVWQPHTFSRTRMLIEQFAASFAQADRV
                     IALDIYASREKADPSITTAAVVERMGHANAIHIPNRRDAADYLLERLHPDDVVLTLGA
                     GDGDAIGQWVLEGLLLQQGNKR"
     gene            6793..7746
                     /gene="murB"
                     /locus_tag="PLC06_05220"
     CDS             6793..7746
                     /gene="murB"
                     /locus_tag="PLC06_05220"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRO89878.1"
                     /translation="MKLMTTVQTVERLRQLFGDQLRVNEPLQRHTTARVGGTAELFLA
                     VTTSEELLAAAEVAYNAGVSCFVLGGGSNILIADSGISGLVILNRARRVSFRHSGAGV
                     VCTVESGANLSALARQCISKGLGGLEWAIGIPGTVGGAVVGNAGAHGADIDGQFLAAT
                     IWEPGRGVFVYNREDMKYGYRDSVLKRDQFVGHARRVVLSAELQLKPEPTDVLAARAE
                     GFNAHRKERQPGGASTGSMFKNPENYYAGYLIDTAGLKGFRVGDAAISEKHANFFINE
                     GDATAEDVRSLIAEAWNAVREQFGIEMELEVELVGDWKFES"
     gene            7810..8985
                     /locus_tag="PLC06_05225"
     CDS             7810..8985
                     /locus_tag="PLC06_05225"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRO89879.1"
                     /translation="MTRQTKLRIGVLFGGRSGEHEVSLRSARSVMAALDRDRYEIVPI
                     GITKEGRWVAADVDALASGLVAGDARSATLLPEPADASLMAVDPVPSDPAASSISTIT
                     TLDVVFPVLHGTYGEDGTVQGLLELADVPFVGAGVVGSAVGMDKAIFKQVMTAAGLPV
                     LPWVLCTRNQWRREPDAVIAAVESALPYPVFTKPANLGSSVGISKCRDRDELRAGLDE
                     AARFDRRLVVEQGIHRARELEVAVLGNDEPVASVVGEVRPRREFYDYVAKYMAEPGSE
                     DESELIIPAELAAGQAETIRELAVRAYKVIDCAGLGRVDLLLDDQSGQIYLNEINTIP
                     GFTTISMYPKLWEATGMSYGELLDHLVDLALERHREKADMATSIDVTALQSGQRGVA"
     gene            8982..9962
                     /locus_tag="PLC06_05230"
     CDS             8982..9962
                     /locus_tag="PLC06_05230"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRO89880.1"
                     /translation="MSERKSDGKQRLRKQPRMRRVESTSSTSMPNLSMPKTAKQRKFR
                     NSTQRIRRPLSGVGALVLNTRWLSVAIVALAVYTLYLIGTDERFYLSYIPVEGSSTIA
                     INDIVAESGLAGSHIFAADPQEAADNIREIGGVVTATVTLHWPNEVMIRIREKPPVAT
                     WQEGEKMYWIDADGHLADARAQTVGLLNIISEIPVPAAQAQPASFKDGDETATDEATA
                     TEEAEAVDGEASKTTVDSLAAFVPREVLAGALQLRELRPNIDKLYYKPSDGLGYQDGR
                     GWTAYFGTGRDMHQKLVVYETIVADLLARGARPAYISVANQHKPYYRLAQ"
     gene            9978..11195
                     /gene="ftsA"
                     /locus_tag="PLC06_05235"
     CDS             9978..11195
                     /gene="ftsA"
                     /locus_tag="PLC06_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRO89881.1"
                     /translation="MEEIVFGLDIGTTKICALVGAVRNRELRIIGLGMQPSAGMRKGM
                     IVDVPAASVAVAAAVEAAEQTSGYQLSQALVSIAGEHISSTNNRGLVAINRNDSGVTA
                     QDIDRALETAQAIPIPNNRQIIHLIPRHYTIDDHAGVRQPLGMHGYRLEIEAHIVTAA
                     TSALQNLKHCTERVGISAEEVVLNALASGEAVLEPAEKEMGVLVADIGGGTTDLALYK
                     QGTVWHTHVIPIGGHHITNDIAIGLRAPYDVAEKVKIQYGDCRPADIDPEFVFTVEPF
                     GGERIQVGRQDLAQVIEARVEEIFHMILKDIQQSGYDEYLPAGIVLTGGASLLRGITD
                     VAERVLHVPARVATPKDLVGLVDSLHSPAYATSVGLLRWAITDHHRYQTHAQQSGQWS
                     RKVGSILRTLLPG"
     gene            11258..12445
                     /gene="ftsZ"
                     /locus_tag="PLC06_05240"
     CDS             11258..12445
                     /gene="ftsZ"
                     /locus_tag="PLC06_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRO89882.1"
                     /translation="MNADYRGRDAQRMPATEGSALIRVVGVGGGGSNAVNRMINENIV
                     GVEFIAVNTDQQALLGSNAPIRIAVGERTTRGLGSGGDPETGARAAEESIEELRQTLQ
                     GSDMVFVTAGMGGGTGTGGTPIVARAAREAGALTIGVVTRPFTFEGSQRARSAEAGIE
                     ALKEHVDTLIVIPNDRLLETADKRVSLLDSFKMADDILRQGIQGISELITVPGLINLD
                     FADVKTIMSMGGAALMAVGEASGDERARDAATQALSSRLLDISIDGARGILFNVTGGP
                     DLTLYEINQAAAIIRETAHPDVNLIFGAVIDEKMSNKLRITVIATGFEHNTPMRSMAS
                     VAATQSARMMPNRPPTRESVPANAPAANPPNARPQSQPQGRSQDAYRFNDLEVPAFLR
                     KRQ"
     gene            12590..>13005
                     /gene="nrdR"
                     /locus_tag="PLC06_05245"
     CDS             12590..>13005
                     /gene="nrdR"
                     /locus_tag="PLC06_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRO89883.1"
                     /translation="MRCPYCHYEKTHVIDTSHDRRGGTRRRRVCESCGQRYTSYERPI
                     LATPLLIKKDGSREEFSREKLLGGIRVACAKRPISAADIERIAGEIEAELQRMGQEEV
                     KSRIVGDLVIGKLKELDLVAYVRYAIVYLRLDDLTA"
BASE COUNT         2412 a         3984 c         4154 g         2455 t
ORIGIN      
        1 catggcgatg gatcgcatcg tcaccgcttt agggaggctg gactaatggg ctgggcgctt
       61 accgtggccg ccgccacctt cctgttatcc gtcatctggg gtgcgccgct gatcgagatc
      121 atgcggcggc tcaagttggg caagaatatc cgtatcgatg gcccggccac ccatgccgtc
      181 aagatgggca cgccggccat gggaggcgtt ttaatcatcg gatggaccct tgtggtcagc
      241 cttgtggtga gtatcgtgca gattgttcaa gcgctggaaa ctgccgaaag cgtggtcatc
      301 cccctgggcg tcatggtagc ctactctatc ctcggaggta tcgacgacta ccaggggttc
      361 cgcctcagac caggcgaagg catgcgggcg cgggtcaagc tctggattca actcgccatc
      421 gcgctgatcg ccgcggtcct gctgtactgg ttggtgaacg acaggcatgg ctgggttgcc
      481 gtgccgaccg ttcccctcct gttggatatc ggcctgctct acatcccgat ggccgtcatt
      541 ctgatcacgg ggtccgccaa cgccgtgaac ctgactgacg gcctggacgg attagcgggg
      601 ctaatctcgg ctaccgcctt cgtcgcctat ggcatcatcg cctttctgca ggatcagcag
      661 ttcctcgtcg tgttcagctt catcgtgatc ggcgcgacct tcggcttcct gtggtacaac
      721 gccaaaccgg cgcagatgtt catgggcgac gtaggttcgc aggccctggg cgcggccctg
      781 ggtgtggtgg ccctgatgac gaaccaatgg ctgatcttcc ccatcatcat cgccatcccg
      841 gtggcgacgg aactttcatc gacgctgcag gtgctctact tcaaggccac cggcggcaaa
      901 cggctgttca agatggcccc gctccatcat cacttcgagt tgttgggctg gagcgagacc
      961 caaatcgtgc agcgttggtg gatcatcgcc atgctgacgg cgatgatcgg catcgcgctg
     1021 gcgctggtgt aataatcatg gacgcgctaa acggcaaacg acttgtgata ctcggtatgg
     1081 cccgtcaggg aacggctctg gcgcgttttg ccgtggatgc cggggcgttt gtgacgctgt
     1141 ccgacatgcg ctccgccgaa cgattgggcg cgcaaatgga attgttggcc gatctgcccg
     1201 ccgaccggtt gcgctacgtc ctgggcggtc atccgatgtc attacttgac gagtgcgatg
     1261 tgctggccat cagcggcggc gtgtccacag acatgccgct tgtcgttgag gccacgcgga
     1321 gaggtatccc gctgaccaac gattccctgg agttcatgcg ccgcgtgccg gctctggtca
     1381 tcggcatcac cggctcggcc ggcaagacga caaccacggc tctcaccggc cagatgggcc
     1441 aaaagtccga ccgtcgaacg tggatcggcg gcaatatcgg ccgccccctc atcaccgacc
     1501 tgccccaaat atcggccgac gacctggtgg tgcaagagct gtccagcttc caactggaga
     1561 tctgggacca ttgcagccca ccggtggccg ccatcctgaa cgtgacgccc aaccatctgg
     1621 accgccacaa gacgatggca agttatgcgg cagccaaaag ccatatcctg cgctcacagc
     1681 gatcggatgg catcgccgtt ctctcggccg acgatcccgg ttcgctggag atgcgcgagt
     1741 ttgtgcgtgg tcgcctgcgc acgttcagcg cccaccagcc ggtgacggac ggcgcattcc
     1801 tgcgcgatgg gcagatctgg ttgaatgacg accagggcga gcattgggtt tgccccgtga
     1861 ccgccagccg gctcatcggc ccgcataata tcctgaatat tctggccgcc gtcgctctgg
     1921 ccgattcggc cggcgtgccg cgcgaatcta tcgccgaggg catccgcctg ttcgacggcg
     1981 tgccccaccg ccttgagatc gtgcgccggg tgaacggcgt gcagtacgtc aacgattcga
     2041 tcgccaccgc gccggagcga gcgctggcgg cactggcagc gttcgacgag ccggtgattc
     2101 tgctggccgg cggccgcgac aaggatatgg cctggggcca atggggacgg caagtgagtc
     2161 gccgcgtgaa gcatgtggtg cttttcggcg aactggcccc attgctggcc cgccatctga
     2221 cggattccgt ctcctcgacg cgggtcgata cgctggctga agccgttcag gtggcccatg
     2281 ccgaagcctc ggcgggtgat gtggtgctcc tgtcgcccgg cggcacgagt tttgacggct
     2341 acaccgattt tgtagagcgc ggcgagcatt tccggttatt agtgagagag ctggagtaag
     2401 tggaggtcat ccgatggcag caataaacgt catcaagaaa cgaggtacat cgctatgggc
     2461 cggacgcaac aagctggctg tcgactactg gctgctgttg gcgaccggcg cgctactggt
     2521 cgttggcttt ttaatggtct acagcaccac gtttgacctg ggcgaccgtt acgaagggaa
     2581 tccggtttac ttcgtcacac ggcagctgac tgccatgggg ctaggagtcg cggtggtgat
     2641 cgtcctgctg cagttcgact atcacatcct gcgccgcatc tccgtgccgc tgattgtggt
     2701 gaccatcgtc atcctcctgg tcatgctgtt attcggccag gtcgatgctt acggcgctat
     2761 ccgcacctta tccgacggct cttaccaacc ctcagagctg gccaagttgg cgatgatcct
     2821 ttacatcgct cattggctgg actcaaaggg cgagcgaatc aagaccctca actacggtct
     2881 gttcccgttt gcggccatca ccggcatcgt ctgcggactt atcgtcctgc agccggcgct
     2941 cagtacagcc atcctcattg gcctgattag tttcacgctc ttcttcgtgg ccggagccga
     3001 tctgaagcag gtactggcgg ccggagttgt ggccatggtc atgctgttct tcctgatgtt
     3061 cgcgctggat catgcctcac aacgcatcaa cgactacatg gcggccttcc gcgatccgca
     3121 gcaagccggc taccaggtga agcagtcgct ggctgcgctg gctaacggcg gctattgggg
     3181 cgttggcctg ggcaaaggcg atcagaaatt cgggccgctg cccctggctc ataccgacgg
     3241 cgtattcgct attgtgggcg aggaaatggg gctgttaggc tctatcggcg tcgttttact
     3301 gttcgctttc atggcctggc gcggcttcct ggtggccacg cgcgcccgtg acacctacgg
     3361 tttcttgctg gccgtcggcg tcacggcctg gatttcctac caggcgctca tcaatattgc
     3421 ggtaatcacg gccgtcatcc cgttcacggg gatgcccatg ccgttcctaa gctatggtgg
     3481 ttcgtccatg ctgacgacga tcatcggcgt cggcatattg ctctgtgtct cgcgtgacgc
     3541 ggccatgggc cgccagtctg cccggcggca gccgaagagc agttgatggg actgatatgc
     3601 gagtattggt ttgtgccggt ggtacgggcg gcggcatcta tcccgcgttg gcagccgtga
     3661 cggctttgca ggcgcagggt gtcggcgacg acgatatatt gtggatcggc acaaaaggtg
     3721 agatggaaga aacgttggta ccaagagcag gtttacgact ggagaccatt ccgggcggcc
     3781 ccatcgttgg ggtgccatta ggcaccagag cgcgtaatgc cgtccggctg gggcgcggca
     3841 tcggggttgc ttccggtcat gtgcgccgtt ttcggccgaa tgtcatgttc atgaccggcg
     3901 gctatatggc cgcaccggtg gcccttgccg cccgacgcca cggcgtcccc atcgtcatct
     3961 atctgcctga cgtggaaccc ggcagttcga ttcggtttgc cgtgcccatg gcccggcgga
     4021 tcgcctgcac caccgatgga tcggcggcgt tcgtgcccca ggaaaagatg gtcgtcaccg
     4081 gctatccggt tcggcccgat attcgcgcgg ccaagtcgct cacccaggca gaggcactgg
     4141 cgaaattcaa cctgcaaccc gggcgaccga cgctgttcgt cttcggtggc agccgcggcg
     4201 cacgcaacat caatcaggca ttgatggccg ccctgcccgc actgctgacc cgcgcgcaag
     4261 tcatccacat cagcggcacg ctgacgtggc cggaggtgga agcgaatgcg gcgacgctgc
     4321 cggccgactt gcaggcgtgg tatcggccgt atccttatct ccacgaggag atgggcgcgg
     4381 cttttcgagc ggccgacctg gcactggcgc gcgccggagc cagcatgctg ggcgaaagcc
     4441 cggcgttcgg attgccggcc attctggtgc cgctgacctt tgcctggcgc tatcagaagg
     4501 tgaacgccga ttatttgacg gaacgcgggg cggccgtgca gctcatcgac gagacgctgg
     4561 ccgaaacgct cctgccgacc gttcaggatt tgctgtttga ttcaaataaa ctggctcaaa
     4621 tggccacggc cgcgaccatg ctcgacagac cggatgcggc gaccgatttg gcccaggtga
     4681 tacgctcgga agcaaagccg gagaagaagc cgacttcgac gctcccacag gaggagacat
     4741 catgttgagt ttaaccacag ctttctgggg tctcgttgtt ctgtttgccg tcgccggaat
     4801 gatgcgtggc tggaccaggg agatcgtcgt caccgcgtcg atcattctgg ccttgttcgc
     4861 catcaatcaa ttgatgagcc caatcttcgg tcttctcaac tgggacaaca acgcagtcca
     4921 gcccgacaat ataaggcgct atcagttctt catcatgtcg tctttcctgc tgctattggc
     4981 tttcgccggt tatcagggtc cgactttggc cagcagccgg gtgggcgacc gcctgaaccg
     5041 ccgcgacaac ttgcaagaca agctgttagg tttatccgtc ggcgcaatta acggctggct
     5101 catcatcggc tcgctctggt cttttctgga gtacaaaatt atcgcggcta acacctggct
     5161 ccgttttccg cctaatattg gctatccatt tgacgtatca agaattaccc gaccagcacc
     5221 ggaactggcg ccgatcatcg acaatctgcc tatcccgttt ctcacccaat cgccgtatat
     5281 tttgttgctg ctattgattt tgttcgttct gtttctgctg atcgttctgc tgtagatcgt
     5341 catggaatca ttgatggaga gagtcgtggg tactcaactc acccctggaa tgcatatcca
     5401 catcgtgggt atcggcgggg ccggcatgtc ggccatcgcc cgtgtcctgt tggggcgcgg
     5461 cttccgggtg tccggctccg acaagcaaag caatgcgcaa acggccgccc tggccgccga
     5521 gggcgtgacg gtctatgccg ggcacgcggc cgaacatatt gatggagccg acctggttgt
     5581 catctcctcg gccataccgc acgccaaccc ggaatgggtc gccgccgagg cgcgcggatt
     5641 gcctgtgctc aagcgcgccg atctgctggg cgtgttgatg caagggtctg tcggcgtggc
     5701 cgtggccggt tcgcatggca agaccacgac caccggcatg atcgcccaca tcctgatgga
     5761 gtgtgacctg gacccgaccg tcatcctggg cggcacgctg gccgaactgg gcggcaacgg
     5821 gcgttatggc gaaggccccc acttcgtcgt tgaagccgac gaatatgact acatgttcct
     5881 cggcttgcga cccgaagtag cggtcatcac caacgtggaa cacgatcacc ccgacctgtt
     5941 cccgacggcc gacgcctacc gggaagcatt cggccgattc gtggcgttgc ttccatcagg
     6001 cgggcggctt atcatttgcg ccgacgatcc cggcgcggcc gacctgttga ccgagcttga
     6061 acccggcaat gtcaccgtca cgacttacgg gctgggcgaa tcggttaccg gcatcgtgcc
     6121 cacggtgcgc gcgctggaca tgcgcccgaa tcagatgggc ggcagcgatt tcgtggtcga
     6181 gaacggtgga caaacggtcg ggctggcgcg gattcgcctg ccgggagcgc acaacgtgcg
     6241 taacgctctg gcggcgctca ccgtggcgat gaatctgcaa atcgacttct ctccggcctg
     6301 ccgagcgctg gccgggtttg gcggcgtaca gcggcgattc cagctcatcg gcgaagtcgg
     6361 cggcgtgacg atcatcgacg actacgccca tcatccgacg gagatcaagg ccacactggc
     6421 tgcggcacgg caacggtttc cggggcgtcg actgtgggcc gtctggcagc cgcacacatt
     6481 cagccgcacg cgcatgctga tcgagcagtt tgcggccagc ttcgcccagg ccgaccgggt
     6541 gattgcgctg gatatttacg ccagccgtga aaaggccgat ccgtccatca ccacggccgc
     6601 cgtcgttgaa cggatgggcc atgcgaacgc gatccacatc ccgaaccggc gcgacgcggc
     6661 cgattatttg ctggaacgcc tccatcccga cgacgtcgtg ctgacgctgg gcgcgggaga
     6721 tggggatgcg atcgggcagt gggtattgga aggattactg ttgcaacaag ggaacaagag
     6781 ataatcagcc acatgaaact catgacgact gtccagacgg tagaacggtt gcgccagcta
     6841 tttggcgatc aattacgcgt aaatgagcct ttgcagcgcc atacgaccgc gcgagtcggc
     6901 ggcacagccg agttgttcct ggccgtaacc acgtcggaag agctgctggc ggccgcggaa
     6961 gtggcctaca acgccggcgt gtcgtgcttc gtgctgggcg gcggatcgaa tatccttatc
     7021 gccgattcgg gcatcagcgg cctggtcatc ctgaaccggg cgcggcgcgt cagcttccgc
     7081 cacagcgggg caggcgttgt ctgcaccgtc gaatcgggcg ccaacctgtc ggcgctggcc
     7141 cggcaatgca tcagcaaggg actgggcggc ctggagtggg ctatcggcat ccccggcacg
     7201 gtcggcggcg cggtggtcgg caatgccggc gcgcatggag ccgacatcga cggccagttc
     7261 ctggcggcca ctatctggga accgggacgc ggcgtgttcg tctacaaccg cgaggatatg
     7321 aaatacggct accgtgacag cgtcctgaag cgcgaccagt tcgtgggtca cgcgcgccgg
     7381 gtcgtgctgt cggccgagct gcagctgaag cctgaaccga ccgacgttct cgcggcccgc
     7441 gccgaggggt tcaacgccca ccgcaaggag cggcagccgg gcggggccag caccggctcg
     7501 atgttcaaga acccggaaaa ctactatgcc ggctatctga ttgatacggc cgggctgaaa
     7561 ggctttcgcg tcggcgacgc ggcgatctcg gaaaagcacg ccaacttctt catcaatgaa
     7621 ggcgatgcga cggccgagga tgttcgctca cttatcgccg aagcctggaa cgccgtccgc
     7681 gagcaattcg gtatcgagat ggaactggaa gtcgaacttg tcggcgactg gaagttcgaa
     7741 tcatagcaaa tcaggaatga tatcaactga cctacggatc actaacttac tgattactga
     7801 cctactgata tgaccaggca aaccaaactc cgcatcggcg tcctcttcgg cgggcgctcc
     7861 ggcgagcatg aagtatcgct tcgttcggcg cgctcggtga tggctgccct cgaccgcgac
     7921 cgctacgaga tcgttcccat cggcatcacc aaagaggggc gctgggtggc agccgacgtc
     7981 gacgcgctgg cgtcggggct ggtcgccgga gacgcgcgct cggctacgct gctgcctgag
     8041 ccggccgatg cgtcgctgat ggccgtggac cccgtgccca gcgatccggc cgcgtccagt
     8101 atctcgacca tcaccacgct cgacgtcgtc tttccggtac ttcacggaac ctacggtgag
     8161 gatggcacgg tgcagggact gctggaactg gccgatgtgc ccttcgtggg cgcgggtgta
     8221 gtcgggtcgg ccgtgggtat ggacaaggcc atcttcaagc aggtgatgac ggccgcgggg
     8281 ctgccggtgt tgccgtgggt gctctgcacg cgcaatcaat ggcggcgcga acctgacgcg
     8341 gtgatcgccg ccgtcgaaag cgccctgccc taccccgtct tcaccaaacc ggccaatctc
     8401 ggctccagcg tcggcatcag caaatgccgc gaccgcgatg aattgcgggc cgggctggac
     8461 gaagccgccc gctttgaccg gcggctggtt gtcgagcagg gcatccaccg ggcgcgcgag
     8521 cttgaggtgg ccgtactggg caacgacgag ccggtggcca gtgtggtggg cgaggttcgg
     8581 ccgcggcgtg aattctacga ctacgtggcc aaatatatgg ctgagccagg ctccgaggac
     8641 gagtcggaac tgatcattcc ggccgaactg gcggccggac aggcggagac catccgcgaa
     8701 ctggccgtga gggcttacaa ggtgattgat tgcgccgggc tggggcgcgt cgatctgctg
     8761 ctggacgacc agtccgggca gatctatctg aacgaaatta acaccattcc gggcttcacc
     8821 acgatttcga tgtacccgaa gttgtgggag gcgacaggta tgagttacgg cgagctgctg
     8881 gaccatttgg tcgatctggc gctcgaacga caccgggaga aggcggatat ggcgacgtcg
     8941 attgacgtca ctgccctgca atcgggtcag cgaggggtgg catgagcgag cgaaagagcg
     9001 acggcaaaca acggctgagg aagcaaccgc gcatgcgccg ggtcgaatcg acatcgtcaa
     9061 cgtcgatgcc gaatctgtcc atgcccaaaa cagccaagca acgcaagttc cgcaacagca
     9121 cgcagcgcat tcgccggccg ctttcgggcg tcggggctct cgtcctgaat acgcgctggc
     9181 tcagcgtggc catcgtggcc ttggcggtct ataccctgta cctgatcggc accgatgagc
     9241 ggttctatct gagctacatc ccggtcgaag gctcatcgac catcgccatc aacgacatcg
     9301 tggccgagag cgggctggcc ggcagccata tcttcgccgc cgacccgcag gaagcggccg
     9361 acaatatccg cgagattggc ggcgtcgtca cggctaccgt cacactccat tggcccaacg
     9421 aggttatgat ccgtattcga gagaaaccac cggtagccac gtggcaggaa ggcgaaaaga
     9481 tgtactggat cgacgccgac ggccatctgg ccgacgcccg cgcgcaaacg gtgggcttgc
     9541 tgaacatcat ctccgagatt ccggtccctg ccgcgcaggc ccaaccggca tctttcaaag
     9601 acggggatga gacggcaacg gatgaggcca ccgccacgga agaagccgag gcggtcgatg
     9661 gtgaagcgag caaaacaacg gtcgacagcc ttgcggcatt cgtgccgcgc gaagtgctgg
     9721 cgggcgcgct gcaattgcgc gagttgcgcc ccaatatcga caagctctat tacaagccgt
     9781 ccgatggttt ggggtaccag gacggccgcg gctggactgc ctatttcggc acaggaaggg
     9841 atatgcacca gaaactggtt gtctacgaga cgatcgtggc tgatttgttg gcacgcggcg
     9901 cccgaccggc gtatatcagc gtcgccaatc aacacaagcc atattaccgg ttggctcagt
     9961 agacctggag acaggttatg gaagagatcg tatttggtct ggatatcggc acaaccaaga
    10021 tatgtgctct cgtgggcgcg gtgcgcaacc gcgagctgcg gattatcggc ctcggcatgc
    10081 agccgtcggc cggcatgcgc aaggggatga tcgtcgatgt ccccgcggcc tcggttgcgg
    10141 tggcggcggc cgtggaagcg gccgagcaga catcgggcta ccaactctcg caggcattgg
    10201 tcagcattgc cggggagcac atcagctcca ccaacaatcg cggactggtg gccatcaatc
    10261 gtaacgacag cggggtgacg gcgcaggaca tcgaccgcgc gctggagacg gcgcaggcga
    10321 tccccatacc caacaaccgc cagatcatcc acctcatccc gcggcactac accatcgacg
    10381 accatgccgg ggtgcggcaa ccgttgggca tgcacggcta ccggctggag atcgaggccc
    10441 acatcgtcac cgcggccacc tccgccttgc agaacctgaa gcattgcacg gagcgagtgg
    10501 gcatcagcgc cgaagaagtg gtgctcaacg cgctggcctc cggcgaggcg gtgctggagc
    10561 cggccgagaa ggagatgggc gtgctcgtgg ccgatatcgg cggcggtaca accgacctgg
    10621 cgctctacaa gcaggggacg gtctggcaca cccacgtcat cccgatcggc ggccaccata
    10681 tcaccaacga catcgctatc ggcttgcgcg cgccctacga tgtggccgag aaggtgaaga
    10741 ttcagtacgg cgactgccgc ccggccgaca tcgacccgga gttcgtcttc accgtggagc
    10801 cgttcggggg cgagaggatt caggttggcc ggcaggattt ggcccaggtc atcgaggccc
    10861 gggtggaaga gatcttccac atgatcctga aagacatcca acagtcgggt tacgacgagt
    10921 atctgccggc gggcattgtc ctgacaggcg gggcttcgct gctgcgcggc atcaccgacg
    10981 tggccgagcg cgtgctccac gtgccggccc gggtggctac gcccaaagac ctggtcggcc
    11041 tcgtcgattc attgcacagc ccggcctacg ccaccagcgt gggcctgctg cggtgggcca
    11101 tcacggacca ccatcgttat cagacccatg cccagcaatc cggccaatgg agccggaaag
    11161 tgggcagcat cctgaggacc ttactgccgg ggtagtggcc ggcagattgt caaacaactt
    11221 gcttattgga aaactttagt tagaggggtt aagagatatg aacgcggatt acagagggag
    11281 agatgcacag cgcatgccgg ccactgaagg gtcggccttg atccgggtcg ttggcgtggg
    11341 tggcggcggc agcaatgccg tcaaccgcat gatcaacgaa aacatcgtgg gcgtcgagtt
    11401 catcgccgtc aacaccgatc agcaggcatt gctcggcagc aacgcgccca ttcgcatcgc
    11461 cgtcggcgaa cgcacgacgc gcgggctggg ttcgggcggc gacccggaga cgggcgcgcg
    11521 cgcggccgag gaatcgatcg aggagctgcg ccagacgctg caaggctccg acatggtctt
    11581 cgtgaccgcg ggcatgggcg gcggcacggg caccggcggc acgcctatcg tggcccgcgc
    11641 ggcccgcgag gccggcgcgc tgaccatcgg cgtcgtcacc cgccccttca cctttgaagg
    11701 atcgcagcgg gcgcgttcgg ccgaggccgg aatcgaggcg ttgaaggagc atgtcgacac
    11761 gttgatcgtc atccccaacg accgcctgct ggagacggcc gacaagcgcg tgtccctgct
    11821 cgattcgttc aagatggccg acgacatcct gcgccagggc atccagggca tcagcgagct
    11881 gatcaccgtg ccgggcctca tcaacctgga cttcgccgac gtcaagacga tcatgtcgat
    11941 gggcggcgcg gcgctcatgg ccgtgggcga ggcctcgggc gacgagcgcg cccgcgacgc
    12001 cgccacccag gcgctgtcga gccgtctgct ggacatctcc atcgatggcg cgcgcggcat
    12061 cctgttcaac gtgaccggcg gccccgacct gacgctgtac gagatcaacc aggccgcggc
    12121 catcatccgc gagacggcgc acccggacgt caacctgatc ttcggcgccg tcatcgatga
    12181 gaagatgtcg aacaagctgc gcatcacggt catcgccacc ggttttgagc acaacacgcc
    12241 gatgcggtcg atggcctcgg tggctgcgac ccaatcggcg cgaatgatgc ccaaccggcc
    12301 gccgacccgc gaatcggttc cggccaatgc gccggcggcc aatccgccca acgcgcggcc
    12361 gcaaagccaa ccgcagggac gcagccagga cgcctaccgg ttcaacgatc tggaggtgcc
    12421 ggcctttctg cgcaagcgac agtaggtatc aacgtggaca ttgtccttcg ccgtctccgt
    12481 gatagaatgg ggagaggtaa cggcatatat tggggtaggc atgcgcctgc cggtacaata
    12541 tatggtcatt acccacccat tcacacggac aggatacgga ggcagggcca tgcggtgtcc
    12601 ctattgtcac tacgaaaaaa cacacgtcat tgataccagc cacgaccggc gcggcggcac
    12661 acgccgccgc cgggtctgtg aatcgtgcgg ccagcgctac acgtcctacg agcggccgat
    12721 cctggccacg ccgctgctga tcaagaaaga cggctcgcgc gaggagttct cgcgcgagaa
    12781 gctgttgggc ggcatccgcg tggcctgcgc caaacggccg atctcggccg ccgatatcga
    12841 gcgcatcgcc ggcgagattg aagccgaact gcaacgaatg ggccaggaag aagtgaagag
    12901 tcgcatcgtc ggcgatctgg tcatcggcaa gctaaaggag ctggacctcg tcgcctatgt
    12961 gcgctacgcg atcgtgtatc tgcggctgga cgacctgacg gccat
//
LOCUS       DAOMQG010000059        12982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2064_length_12982_cov_11.827261, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000059 DAOMQG010000000
VERSION     DAOMQG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2064_length_12982_cov_11.827261"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /gene="ligA"
                     /locus_tag="PLC06_05250"
     CDS             complement(<1..1856)
                     /gene="ligA"
                     /locus_tag="PLC06_05250"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRO89884.1"
                     /translation="MTPEERIQKLRVDIDYHLYRYHVLDSPIISDAEFDALYRELQAL
                     EAEHPELVTPDSPTQRAGAAPLDVFRKVAHPAPILSLMNAFNSDDLVAWRARIGRLLP
                     DGVDLDYVVEPKLDGLTVVLTYRNGVFDQGATRGDGVVGEDVTINLRTLRSLPKRIPI
                     DPHSSLVPPAYFVVRGEVFMPLDQFAALNEERAAAGEPMYMNPRNTAAGSLRQLDPGK
                     TAERPLHLYCYDVVIFEGGDVPGNQWDRLGYLKELGFPVSPDNRYCSSLDEVIQAYDE
                     WTSRRNQINYEVDGVVVKINDRAVADSLGYVGKDPRGAIAMKFPAQEKTTRLLDVQIN
                     VGRTGVLAPNAVLEPVEIGGVTIRNATLHNFDEIARKDIRIGDRVLIKRAGDVIPYVV
                     GPIVDARDGSERPIVKPTQCPFCGEPVVQLPGEVAIYCENASCPEQLIRRVEYFVSRG
                     AMDITSFGTQTAELLYNQGLIRDVADIYTLRRDDLLALEGYKDKKVDNLLAGIEASKS
                     QPADRVLTALGIRFVGSVVATLLIQSLGSVDAIAKATAEELQEIEGIGPQIALSVVAW
                     FRNDHNKTLIEKLRTAGLNFSRGEEPESTGSSLLLGLTFVITGTLPTMSRDDA"
     gene            complement(1904..2440)
                     /locus_tag="PLC06_05255"
     CDS             complement(1904..2440)
                     /locus_tag="PLC06_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO89885.1"
                     /translation="MTKHSEPSTNHSVTKRTGIPWLAVGLGSAVTIVGLLLLGWFLGN
                     FLTQLPEEAAVDAVPTIVLLTAPPVPTATISLKPPTPTIAPTATTAATPDFSVAPAEL
                     TVGYYARVGDTGGVGVTLRNGPSTRNLPVTVAAEGSSLLVLDGPTAGGDYQWWQVRMP
                     NGTEGWAAGDFLVPAAAP"
     gene            complement(2477..3658)
                     /locus_tag="PLC06_05260"
     CDS             complement(2477..3658)
                     /locus_tag="PLC06_05260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRO89886.1"
                     /translation="MMKTYPADTLLTRQGEIEDTFYVLDSGNVIVVRLAEDGEEQMLA
                     TLGPRQSFGEMALIDDKPRLATIKTLTESTVLEMTADQFRDLLRHDPDLALHITRRVL
                     ANLRRLDQVAIQDLREKNDLLHQAYLELQAAQLILVEKKRLEREMELAAEMQRNLLPA
                     ALPSFEDYRFYSYLAPARHVGGDLFDVRPIDEENVAILIADVADKGVHAALLMAVTRT
                     LFFQEAVRSLSPREVVYAVHSALLSIGGGNLEGYGMDAFVTAFYAVLNRPSGKLTYVR
                     AAQDRPLWIRAGARPEPLEGNGRFLGMIDGLELQEHQIMLQPGDNLLLYSDGVTDQMN
                     EADESYGVERLKAAFQAAVGESDGNQLLFIVKDIDNWRGRAPAFDDLTMLLVQAIDRS
                     S"
     gene            complement(3730..5469)
                     /gene="polX"
                     /locus_tag="PLC06_05265"
     CDS             complement(3730..5469)
                     /gene="polX"
                     /locus_tag="PLC06_05265"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRO89887.1"
                     /translation="MNNREVVDTFNQVADMLSIRGDQIHRILAYRKAAESIGSLGRDI
                     NTVFSEGKLTDIPGIGDTLAAKIEEMLTTGHLEFYEKLAKEIPPSLVEMLRIEGLGPK
                     RVKQIYDILKISTVDELAAAAREGKLRDLPGMGAKSEAKILAGIEALNRHGDDRVPLG
                     TAWPIAQQMLAELERVPGVSKAAVGGSLRRMRDTIGDIDLLVAADSPDAVMDRFAALD
                     RVEVLNARGPSRARVTLLNGLGVDLRVLPAERWGTLLCYFTGSKDHNVKLRELALKRG
                     LSLNEHAFTPLDGSGEILCTTEEEVYRTLGLPYIPPRLREDRGEIEAAAAGKLPVLIE
                     LGDVISDLHMHTIWSDGTLSVLEMAQAAKARGLRGIVITDHSVSLGITNGLSIERLRR
                     QAVEIKAADEAMGPDFRVLHGTEMEIRADGTLDYPDEVMAELDFVIASLHTALHQPRE
                     QVTQRLLNAINNPHVDMIAHPTGRLLPDRAGADLDMDAVISAAARTGTILEVNANPAR
                     LDLRDIHVHMAIDSGVKIAINTDAHRAEDFELLHYGVATAQRGWATAADVVNTWPIER
                     LLNYIAEKTIKQP"
     gene            5701..7491
                     /locus_tag="PLC06_05270"
     CDS             5701..7491
                     /locus_tag="PLC06_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89888.1"
                     /translation="MKKNRFLLVLGLLLIAAMVMTACTATSGDVQQAIEQVAPTLQAA
                     ATELAPTIEAVVEEVAPTVEAAATEIAGGAAAEPAGDLMVVSAPDCDYGGLFKEIAAI
                     DPLTVQFTMCAPDPAFPSKVAFTSFAIQPSEYLESTGGTGDLLEHPVGTGPYMVDHWS
                     RGEEVVFAKNPNYWGEQAVADTLVFRWQAESAARLLELQAGSVDGIDNPGPDDFETIS
                     SDPNLQLLERPALNIFYVGFNNVFAPFDNEKVRQAIAMGIDRARIVDNFYPPGSELAS
                     HFTPCSIPNGCAGEDWYEFDPEAARALLAEAGFPDGFETELAYRDVVRGYLPDPNIVA
                     QDIQAQLKENLNITAKINVMESGAFLDAADSGALTGIYLLGWGADYPDMTNFLDYHFG
                     AGASPQFGDKFPDLTDVLRQAASLAGDDARAPLYEQANNLIKQHVPMVPVAHGGSGVA
                     YLASVQNAHVSPLGNEAFSHMDPGKDTFVWMQNAEPISLYCADETDGESLRACEQVTE
                     ALLAYEVGGTAVVPGLATGCEPNEDLTVWTCTLREGVTFHDGSALDANDVVMTYAVQW
                     DAANPLHVGNTGAFSYFSALWGGFLNAPSE"
     gene            7629..8708
                     /locus_tag="PLC06_05275"
     CDS             7629..8708
                     /locus_tag="PLC06_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89889.1"
                     /translation="MTQYTIRRLVAAIPVVVGVLIVTFLLARAIPGEPCTAMLGEKAT
                     PAVCEVFNREQGLDKPLSTQFLIFARDMMRGDFGNSIRFKRPVSQILIERLPQTIELG
                     AAALAIAVIVGIPLGIISAVKRNSPIDVITMIGANIGVSMPVFWLGLMLAYIFSLLLK
                     DTIFQLPPSGRLSAGLQSIPFYEVFNLEFTAGTFKASLYQFLSNFYIFNSIITADWKI
                     LVDTIRHLILPSVALATIPLAIIARMTRSSMLEVLGQDYVRTAKAKGLSQNQVVMKHA
                     FRNALLPIVTIVGLQLGAVLSGAILTETIFGLSGVGRSLYEAITARDYPIIQGFVVVI
                     AVGYVMVNLLVDLSYAVLDPRIQLD"
     gene            8715..9710
                     /locus_tag="PLC06_05280"
     CDS             8715..9710
                     /locus_tag="PLC06_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89890.1"
                     /translation="MSLTTEPNPPPVVSLNLLDDPSAGDISDFRSNSLWRLTIRRLFK
                     QRSAVVGMILLSLLVFTAIFASVIAPYDPELVLIGKEDVRKRDAPCIHLLGCPAEKPQ
                     HILGIDGNIRDQFSRLIYGTRVSLYVGIITVSFAVIVGTFLGAIAGFFGGIVDNLIMR
                     VLDVVLAFPFLLLAIAIVSVIGPGLRNAMLAIAIISIPVYARVTRASVLSVREMHFIE
                     ASRALGASNWQILFGRILPNSLPPLIVVATLGIATAILDTAALSFIGLGAQPPTPEWG
                     SMLAIERNQVFSAPHLVFFPGLAIMITVLAFNLLGDGLRDALDPRLVHGGGAAKE"
     gene            complement(9812..10036)
                     /locus_tag="PLC06_05285"
     CDS             complement(9812..10036)
                     /locus_tag="PLC06_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRO89891.1"
                     /translation="MTGKYIGRVKWYNTKKGYGFIVRGAGEEIFFHKSATVGEVEEFE
                     EGQWVLYDVETTNKGPEASDVEPYTGEPLV"
     gene            complement(10342..12840)
                     /gene="gyrA"
                     /locus_tag="PLC06_05290"
     CDS             complement(10342..12840)
                     /gene="gyrA"
                     /locus_tag="PLC06_05290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRO89892.1"
                     /translation="MEVGLIKQVNIDQEMRESYLSYAMSVIVSRALPDARDGLKPVQR
                     RILYAMYDMGLRPDTPYRKSARVVGEVLGKYHPHGDQSVYEAMVRMAQDFSMRYMLVD
                     GQGNFGSIDGDAAAAMRYTEARMSRMGYDLLEDIDKNTIDFQSNFDDSLKEPSVMPAT
                     IPNLLVNGASGIAVGMATSIPPHNLTEVVGALIYMLENWAKLDDISLADLMQFIQGPD
                     FPTGGLIARHRGEGDDADVIASAYATGRGRVTVRARAHIEQMGRNKSRIIITELPYQV
                     NKTNLMGRIADLHRDGKIEGLTDLRDESDRRGLRIIIETTRTVDAESVLADLFRLTPL
                     QSTFSISLLALVNSEPRVLTLKQALRHYLEHRLVVVRRRSEYELDKAKQRAHILEGLL
                     IALDHLDEVIDLIRRSRSADTAKTNLIERFKLTEIQAQAILDMQLRRLAALERKKIQD
                     EYKEKLALIKDLEKLLAHPELMRQQIKKELLEINEKFGDKRRTQIVKTVDGGVLTSAG
                     LLPDEEGWVMIGEKGTICRYNGDTLPSFPRKPIEQPAVLVRASTRDTLYLFAASGRAV
                     GIPIHRIPEGDEVGKGTPWGNLTPLGRGDHLVAAVALSADGGQDGYLFLTTLAGVVKR
                     VRLEDLPGVTADSFTVINVAEDDSLGWAKITTGDQGILLATSSGQAIRFNEDAVRPMG
                     LPASGVLGIKFDSDVDGVIGMDVVMPNSFVWSITDDGLAKATPIEEYPLQNRYGQGVI
                     NMRLPKGSSEVAASTLLTRGMTLIVTTSGGVTKKIGLSDTTIGTRSVRPQSVMTLAAK
                     SRVTGAVLPVVNGQLSKTNDASEPKKKRTKSKSD"
BASE COUNT         2771 a         3843 c         3411 g         2957 t
ORIGIN      
        1 gcatcgtcgc gcgacatcgt gggcaatgtg ccggtgataa caaacgtgag acccaggagg
       61 aggctggaac ccgtggattc aggctcctct cctcgtgaga aattaagccc cgcagtcctc
      121 agcttttcaa tcaaggtctt gttgtggtcg tttctgaacc acgcgactac cgagagagca
      181 atttgtgggc caatgccctc tatctcctga agctcctcgg cagtagcctt ggcgatggcg
      241 tcgacgctgc caagtgattg aatgagaagt gttgcgacca cactgccgac aaaccgtata
      301 cccagcgcag ttagcactct atcggccggc tggctcttgc ttgcctcaat cccggccagc
      361 aaattatcga ctttcttgtc tttatagcct tccagcgcga gcagatcatc ccttcgaagt
      421 gtgtaaatat cagcgacatc ccggatgagg ccctggttgt atagtagttc tgcggtttgc
      481 gtcccaaagc tggtgatatc catcgcgccg cgactgacaa aatactcgac ccgacgaata
      541 agctgctcgg ggcatgaagc gttctcacaa tagatagcca cttcgccggg cagttgcacg
      601 accggttcgc cgcaaaacgg gcactgtgtt ggtttgacaa tgggacgttc gctgccgtct
      661 cgggcatcga cgataggccc gaccacatac ggaatcacgt caccggctct cttgatcagc
      721 actcgatcgc cgatgcgaat atccttgcgc gcgatctcgt cgaagttatg gagggtggca
      781 ttcctgatcg tcaccccacc tatctcgact ggctcaagga cggcgttggg cgccagcacg
      841 ccggtgcggc cgacattgat ctggacgtcc aacagtctcg tcgtcttttc ctgtgccggg
      901 aacttcatgg cgatcgcccc gcgcgggtct ttgccgacat accccaggga gtcggcgacc
      961 gcgcgatcgt tgatcttgac gaccacgcca tccacttcgt aattgatctg gtttcggcgc
     1021 gacgtccatt catcataagc ctggatcact tcatccagac tggaacaata gcggttatcc
     1081 ggcgatacgg ggaaccccag ttccttcagg tatccaagcc gatcccattg gttgccggga
     1141 acatcccctc cctcaaagat cacgacatca taacagtaca gatgcaaagg tcgttcggcc
     1201 gttttacccg gatcaagttg tcgcaacgag ccggcggccg tattccgggg gttcatgtac
     1261 atcggctcgc cggcggccgc tcgttcctca tttaatgccg caaactgatc gagcggcatg
     1321 aagacctcgc cacgaaccac aaagtaagcg ggtgggacaa gactgctgtg tggatcaatg
     1381 gggatgcgtt tgggcaaaga gcgcaatgtc cgcaggttta tggtgacgtc ttcgccgacg
     1441 actccatctc cgcgcgtggc tccctgatca aatacaccat tcctataagt caggacgaca
     1501 gtcagcccat ccagcttggg ttcgacgaca tagtccagat cgactccgtc gggaagcaat
     1561 cgccctatcc gcgctcgcca ggccaccagg tcatctgaat tgaaggcatt catcaaactc
     1621 aggatgggag ccggatgagc gaccttcctg aatacatcca gcggcgccgc ccccgctctt
     1681 tgggttggcg aatccggcgt caccaattcg ggatgttcag cctccaaggc ctgtagctcg
     1741 cgatacaagg catcaaattc ggcatcacta atgatcggcg aatcgaggac gtggtatcga
     1801 tagaggtggt aatctatgtc tacgcgaagt ttctgtatgc gctcttcagg agtcatatca
     1861 catgaaccta gttcgataat gaccgatagt gcggcgttcg gacttacgga gcagcggccg
     1921 gtaccaaaaa gtccccggcc gcccagcctt cagtgccatt gggcattcgc acctgccacc
     1981 attgataatc accacccgcc gttggtccgt ccagaacgag aagcgatgac ccttcggcag
     2041 ccacagtgac gggcaaattc cgcgtactcg gcccattgcg aagcgtcacc ccaactccgc
     2101 cggtatcccc taccctggca taatagccga cggtcaattc agccggggcg acggaaaagt
     2161 ccggggtagc cgccgtagtc gccgtcggcg ctatcgtggg ggtcggtggc ttcagcgata
     2221 tcgtcgctgt aggaacaggg ggggccgtca ggagtacgat ggttggaaca gcatcgaccg
     2281 ccgcttcttc aggcagttgg gtgaggaaat tgcccaggaa ccagcccagc aacaataatc
     2341 cgacaatagt aaccgcgctt cccaacccca ccgccagcca tgggatgcca gtcctttttg
     2401 tgacggagtg gttcgtggaa ggttcagaat gtttagtcat ataacctgat atttgcgggt
     2461 cacacgggca aaagggtcat gaactccgat cgatcgcctg gaccagtagc attgtcaaat
     2521 catcaaacgc gggagctcgg ccgcgccaat tatcaatatc tttgacgata aacagcaatt
     2581 gatttccatc agactcgcca acggccgcct gaaatgccgc ctttagccgc tcaacgccgt
     2641 acgattcgtc cgcctcattc atctgatccg tcaccccgtc gctgtaaagc aggagattgt
     2701 ctcccggctg gagcatgatt tgatgctcct gcaattcaag accatcaatc atgcccagga
     2761 atcgaccgtt gccttccaga ggttccggcc gcgcaccggc gcgtatccac agcggccgat
     2821 cctgtgcagc gcgaacatag gttaacttgc cgcttggtcg gttcagcacg gcgtaaaacg
     2881 cggtcacgaa tgcatccatg ccatagccct ctaaattgcc gcctccaatt gacagcaaag
     2941 cgctatgaac cgcgtaaacg acctctctgg gcgataagga acgcacagct tcctggaaga
     3001 acagcgtgcg cgtcacggcc atcaacagcg ccgcatgaac gcccttgtca gctacatcgg
     3061 caatcaatat agcgacgttt tcttcatcga tcggccgcac atcaaacagg tctccgccaa
     3121 catgccgggc cggagccaga taggagtaaa agcgataatc ctcgaatgac ggcagagccg
     3181 ccggcaacag gttgcgctgc atctcggccg ccaattccat ttccctttcc aggcgctttt
     3241 tctccacaag aatcaattgc gccgcctgaa gctccaggta ggcctgatgc agcagatcgt
     3301 ttttctcccg caagtcctga atggcaactt gatccaggcg gcgaagatta gccagaacgc
     3361 ggcgggtgat atgcaatgca aggtcgggat catggcgcaa caagtctctg aattgatcgg
     3421 cggtcatctc cagcaccgtc gattcggtca gggtttttat tgtggccaag cgcggcttgt
     3481 catcaatcaa ggccatttcg ccgaaagatt gccgcggccc cagcgtcgcc agcatctgct
     3541 cttcaccgtc ttcagccagg cgtaccacaa tcacattacc gctatcgagc acatagaacg
     3601 tatcctctat ctccccctgg cgtgtcaaga gagtatcggc tggataggtc ttcatcatcc
     3661 ctacctggcg aattaccgat aacgtatggg gatcgaggaa tgtagccggg tcttctacac
     3721 ttggctgtat catggctgct ttatggtctt ttcggcaata tagttcaata atcgttcgat
     3781 gggccacgtg ttcacgacgt cggccgctgt tgcccaaccc cgctgggcag tcgccacgcc
     3841 atagtggagc agttcaaaat cctcggcgcg atgcgcgtcc gtattgatgg caattttgac
     3901 gccactatca atagccatat gcacgtgaat atcgcgcaga tcaaggcgtg caggattggc
     3961 attgacctcc aaaatggtcc cggttctcgc cgcggccgag atgacagcat ccatatcaag
     4021 gtctgctccg gcgcgatccg gtaacaggcg cccggtggga tgagcaatca tatccacatg
     4081 aggattattg atggcattca agagccgttg cgtcacctgc tcgcgcggtt gatgcaatgc
     4141 agtatgcaag ctggcgatga caaaatccag ctccgccatt acttcatctg gataatccaa
     4201 tgtcccgtcg gcccggattt ccatttctgt cccatgcagc acgcggaaat ccggccccat
     4261 tgcttcatcg gcagccttaa tttcaaccgc ctgccgacgc aatcgctcaa tggacaagcc
     4321 gtttgtgatg ccaaggctca ccgaatgatc ggtgatgaca attccgcgca gcccccgcgc
     4381 tttggcagcc tgtgccatct ccagcaccga cagcgtcccg tcggaccata tggtgtgcat
     4441 gtgcaggtcg ctgatcacgt cacccaattc gatgaggaca gggagcttgc cggccgcggc
     4501 cgcctctatc tcaccccgat cctcgcgcaa tcgtggcggt atataaggta gtcccaacgt
     4561 ccgatagacc tcttcctcgg tcgtgcacag aatttcaccg gagccatcga gcggtgtgaa
     4621 agcatgttca ttcaggctaa gtccgcgctt gagggccaac tcgcggagct tgacattatg
     4681 atccttgctg ccggtaaaat agcaaagcaa agtgccccac cgctccgcgg gcaacacgcg
     4741 caggtcaacg ccaagaccgt tcagcaaggt cacacgggcg cgcgatggac ctctcgcatt
     4801 aagaacctcg actctatcca gagcggcgaa acgatccatg acagcatcag gggaatccgc
     4861 ggccaccagc agatcgatat ctccaatcgt gtcgcgcata cgccgcagag accctcccac
     4921 agcagccttc gaaacgcctg gaactcgctc aagttcggcc agcatctgct gagcaatggg
     4981 ccaggcagta ccaagaggta cacgatcatc accatggcga ttgagcgcct cgatgccggc
     5041 caggatcttc gcctcggatt ttgcacccat tcccggcagg tcgcgtaatt tcccttcacg
     5101 cgccgcggcc gccaattcat caaccgtgct tatcttcagg atatcgtaaa tctgtttaac
     5161 ccgcttcggg cctaatccct cgatacgcaa catctcgacc agtgacggcg ggatctcttt
     5221 ggccagcttc tcgtagaatt ccagatgccc cgtggtcagc atctcctcga tcttggcggc
     5281 caatgtgtcg ccgatgccgg ggatgtccgt cagcttcccc tcggaaaaga cggtgttgat
     5341 gtctcgtcca agggatccaa tactttcagc ggccttccga taggccagaa tacgatggat
     5401 ctggtctccc ctgatggaga gcatatcggc tacttgattg aaagtatcga cgacctcgcg
     5461 gttattcata aaccccatta tacatttatt cccctgtcag aacgctaaat acctctcgat
     5521 aaaccattgg ataaatgttt attcaggtac ctccaacgac tcggcgaaat ggcttgacac
     5581 cgatatgcta tttccgcgtc acttttcatc taaaatcttg cgttttgttt ctatcattgt
     5641 tattctaacc atccttatgg gccaattcgg ctcaaacaaa gaaaggagag atggtaagag
     5701 atgaagaaga atcgattctt gttggtattg ggattgcttc tcatcgccgc catggtcatg
     5761 accgcctgta cagctacaag cggcgatgtc caacaggcga ttgagcaagt agcaccgacg
     5821 ctccaggcgg ccgcgaccga actcgcgccg actattgaag ccgtcgttga agaagttgcg
     5881 ccgaccgtgg aagcggcggc caccgagatc gccggcggcg cggcggccga accggcgggc
     5941 gatttgatgg ttgtatcggc cccggattgt gattatggtg gtctcttcaa ggagatcgct
     6001 gcgatcgatc cgctaacggt gcagttcacc atgtgcgccc ccgacccggc gttcccgtcg
     6061 aaggtggcgt tcacctcgtt cgccatccaa ccgtctgagt atctggaatc gacgggcggc
     6121 accggcgact tgctggagca tcctgtcggc accggcccct atatggttga ccactggtcc
     6181 cgtggcgaag aagttgtttt tgccaagaac cccaattact ggggtgagca agctgtagcc
     6241 gatacgcttg tcttccgttg gcaggccgaa tcggccgcgc gactcctcga gcttcaggcc
     6301 ggatcggttg acggtatcga caaccccggt cccgatgatt tcgagacgat ctccagcgat
     6361 ccgaacctgc agctgctgga gcgtccggcg ttgaacatct tctacgtcgg cttcaacaat
     6421 gtcttcgcgc cgtttgataa cgaaaaggtt cgtcaggcga tcgccatggg tatcgaccgg
     6481 gcacgcattg tcgacaattt ctatcctccg ggttccgaac tggcgtccca cttcaccccc
     6541 tgctccattc ccaatggttg cgccggtgag gattggtacg agttcgatcc tgaggcggcg
     6601 cgcgctttgt tggccgaggc cggcttcccc gacgggttcg agactgagtt ggcttaccgt
     6661 gacgtggtcc gtggctatct gcccgacccc aacatcgtcg cccaggacat tcaggctcaa
     6721 ttgaaggaga acctgaatat cacggccaag atcaatgtca tggagtccgg cgctttcctg
     6781 gacgcggccg atagcggtgc gctgacgggt atttacctgc tgggttgggg tgcggactat
     6841 cccgacatga ccaatttcct cgactaccac ttcggggccg gcgcttcgcc ccagttcggc
     6901 gacaagttcc ccgatctgac cgatgtcctg cgccaagccg cctcgctggc cggtgacgat
     6961 gcccgcgctc cgctgtacga gcaggccaac aacctgatca aacagcacgt gccgatggtc
     7021 ccggttgccc atggtggctc cggcgttgcc tatctggcaa gcgtacagaa cgcccatgtc
     7081 agcccgctcg gtaatgaggc cttctcccat atggatcccg gcaaggacac cttcgtctgg
     7141 atgcagaatg cggagccaat cagcctgtac tgcgctgatg aaaccgacgg tgaatcgctg
     7201 cgcgcttgcg agcaggtcac cgaagcgctt ctggcctacg aagttggcgg aacggctgtg
     7261 gtgccggggt tggccacggg ttgcgaaccg aacgaagacc tgacagtctg gacctgcacc
     7321 ctgcgcgaag gcgtcacctt ccatgacgga tcggcgctgg atgccaacga cgtggttatg
     7381 acctacgcgg ttcagtggga tgcggcgaat ccgctgcatg tcggcaacac gggcgctttc
     7441 tcctacttct ctgctctgtg gggtggtttc ctgaacgctc cttctgagta agcgggaaaa
     7501 tctcaggaaa caaccctgag agagtaacct ggtttttcgc gggatggggt ttggcaagtt
     7561 ttgtcggccc catcccgctc catattcgta gacaatgatc tgagatcgag agagagccgg
     7621 agggtataat gacccaatac acaattcgtc gcctcgtcgc tgcgatccct gtcgttgtcg
     7681 gcgttctaat cgtcaccttc ctgctggctc gcgcaatccc gggagagcct tgcacagcta
     7741 tgctgggcga gaaggcaaca ccagcggtct gcgaagtctt caacagggag caagggctgg
     7801 ataaacctct ttccacccaa tttctgatct tcgctcgtga tatgatgagg ggagattttg
     7861 gcaattcaat tcgcttcaag cgaccggttt cccaaatact gatcgaaaga cttccccaaa
     7921 ccatcgaatt aggcgcggcg gctctggcca tcgcagtcat cgtcgggatt cccttgggga
     7981 tcatatcggc cgtaaaacgc aactccccca tcgatgtaat taccatgatc ggagcaaaca
     8041 tcggcgtttc catgccggta ttttggctcg gcctcatgct ggcttatatt ttctcactcc
     8101 tcctaaaaga cacgatattt caattaccac ccagtggcag gttatctgcc ggtctgcagt
     8161 cgatcccgtt ctatgaggtt ttcaatctgg aattcacagc gggaacgttt aaggcttcgc
     8221 tatatcaatt tctgagtaat ttctatatat tcaactcaat aataacggcc gactggaaga
     8281 ttctcgttga tacaattaga catttgattc ttccatctgt cgccctggca acaatccctc
     8341 tcgccattat tgcacgcatg actcgctcct ctatgcttga agtgctggga caggattacg
     8401 tccgaaccgc caaggccaag ggattatccc aaaatcaggt tgtgatgaag cacgcctttc
     8461 gcaatgcttt gctgcctatt gttacgattg ttggcctgca attaggggcg gttttaagcg
     8521 gggccatcct cacagaaacg atttttggct tgtccggtgt aggcagaagc ctttatgagg
     8581 cgattaccgc gcgagactat ccaattatcc agggctttgt cgtcgtcatc gcagtaggtt
     8641 acgtcatggt gaacctgttg gttgacctgt cttatgcggt actggacccg cgaatccaac
     8701 tggactgacg aatcatgtca ctcactactg aacccaatcc accaccagtc gtctcgctca
     8761 atcttcttga tgatccatca gctggtgaca tcagcgattt tcgctcgaat agcctatggc
     8821 ggcttacaat tcgccggttg ttcaaacagc gctccgccgt cgttggcatg atcctgttga
     8881 gcttgcttgt tttcaccgcc atatttgcat cagtcatcgc cccttacgat cccgaactgg
     8941 tgttgatcgg caaggaagat gtgcgcaagc gtgacgcgcc ttgcatccat ctgttaggat
     9001 gcccggctga aaagccgcag catatcctag gcatcgatgg caatattcgc gaccagttta
     9061 gccgattgat ctatggcacc cgagtgtcct tgtatgtcgg gattatcaca gtctccttcg
     9121 cggtcatcgt cggcaccttt ctcggagcaa tcgccggatt ctttggcgga atcgtcgata
     9181 atctcatcat gcgcgtgctg gacgtcgtcc tggcgtttcc attcttgctg ctggccattg
     9241 ccatcgtcag tgtcattggt cccggcctca ggaatgccat gctggccatc gccatcatca
     9301 gcatacctgt ctacgcccgc gtaacgcgag ccagcgtgct ctccgtcagg gaaatgcatt
     9361 tcattgaggc gtctcgcgcg ctgggcgcca gcaattggca aatcttgttc ggccgcatcc
     9421 tgcccaattc cttaccaccg ctgatcgtcg tcgccaccct gggtattgct acggcgattc
     9481 tggacactgc tgccctttcc ttcatcggcc tgggcgcgca accacccaca cctgaatggg
     9541 gctccatgct ggccattgag cgcaaccagg tgttcagcgc gccccatctc gtcttcttcc
     9601 ccggcctggc gattatgatc acagttctgg cattcaatct tctgggagat ggcttgcgcg
     9661 atgccctgga tccgcgcctc gtccacggtg ggggcgcagc caaggaataa gacgacccca
     9721 tcgtcaatag actccacaaa taataaacga cccggacttt gacatccggg tcgtttttaa
     9781 ttgactcgat tactctccag aatcggaggc gttagacgag aggctccccg gtatagggtt
     9841 caacatccga cgcttcaggg cctttgttgg tcgtttccac atcgtagagg acccactgcc
     9901 cttcttcaaa ctcctcgacc tcaccgacgg tcgcggactt gtggaagaag atctcttcgc
     9961 cggcgccacg aacgatgaag ccataacctt tcttcgtatt ataccatttg actcggccga
    10021 tatatttgcc ggtcatggga tcgatctgaa tggtctcggg gccaacgatt tcagcgccgc
    10081 gcgaaggaga cggctcgctc gattgagcag atttttgata ggttcgctct tccgattcgg
    10141 tacgaggccg atgctcggat cgcgcgggtt ggcctcctcc cgcaagctgc ccaagctctc
    10201 ccaatgccga cggatcgatg ggaagacccg cctggctcaa tcgccgttgc atctcgacag
    10261 taaacacaaa gcgcttgccc tgttcatctt ccacccaacg gtctctaaag ttcatctttg
    10321 ttctcccagt agtgctgcaa tttaatcact cttcgatttc gttcgcttct tctttggttc
    10381 ggaagcgtcg ttggtttttg ataattggcc attgacgacc ggcaggactg cccccgtcac
    10441 acggctcttg gccgccaacg tcatgaccga ttgtggccgc acactgcgcg tgccaatcgt
    10501 tgtatcgctc aggccaatct tcttcgtcac cccaccgctc gtcgttacga tgagggtcat
    10561 cccgcgagtc agcagcgttg aagccgcaac ctcggaagat cccttgggca aacgcatatt
    10621 gatgacgccc tgcccataac gattctgtag cgggtactct tcgatcggcg tcgccttggc
    10681 aaggccgtca tcagtgatac tccaaacaaa gctgttcggc atcacaacat ccatgccgat
    10741 aaccccatcg acatcgctat cgaacttaat gcccaacaca ccggaggcag gcaaacccat
    10801 cggccgaacc gcatcctcat tgaaccgaat ggcctggccg gaagatgtgg ccaacaggat
    10861 cccctgatcc cctgtggtga ttttcgccca gcccaatgag tcgtcctcgg ccacattgat
    10921 cactgtaaag gaatcagccg tcactccggg cagatcttca agtcgcacgc gcttgactac
    10981 gccggccaag gtcgtcagga acagataacc atcctgccca ccatcggccg acagcgccac
    11041 ggccgccacc agatgatcgc cccgtccaag cggcgtcagg ttgccccacg gcgttccctt
    11101 gcccacctcg tcgccttccg ggatccgatg gattggaatg ccgacggctc ggccgctcgc
    11161 agcaaataga tagagcgtat cgcgcgtgct cgctctgact agtacggccg gctgctcgat
    11221 aggcttccgg gggaaagacg gcaaagtatc accgttatac cgacagatcg tccccttctc
    11281 gccaatcatc acccagccct cttcgtcggg taataggccg gcactggtca ggacaccccc
    11341 atcaacagtc ttaacgattt gcgtccgacg tttgtcgccg aatttctcgt tgatctccag
    11401 aagttccttt ttaatttgct gccgcatcaa ttcaggatga gccaaaagct tttccagatc
    11461 cttaataagg gccagctttt ccttgtattc atcctgaatc ttcttgcgtt ccagcgctgc
    11521 cagccggcgc agttgcatgt cgaggatagc ctgtgcctga atctcggtca acttgaaccg
    11581 ctcaatcagg ttcgtcttgg ccgtatcggc cgagcgagaa cgccggataa gatcgatcac
    11641 ctcatccaaa tgatccagcg cgatgagcaa accttccaga atgtgggcgc gttgtttggc
    11701 cttgtccagc tcatattcgc tgcgacggcg cacgaccacg aggcgatgct ccagataatg
    11761 gcgcagcgcc tgttttagcg tcagaacgcg cggctcgctg ttcaccagcg ccaatagcga
    11821 aatgctgaaa gtagattgca aaggtgtcag cctgaacaga tcggccaaca cgctctcagc
    11881 atcgaccgtt cgcgtggtct caataataat acgcaagcca cgacgatccg attcatcgcg
    11941 caagtcagtc aggccttcaa ttttgccgtc gcgatggaga tcggcaatgc ggcccatcag
    12001 attagtcttg ttcacctgat agggcagttc ggtgatgatg atccgggatt tattccgccc
    12061 catttgctcg atatgcgccc gcgcgcgaac agtgacgcgg ccgcgcccgg tggcgtaggc
    12121 ggaggcgatc acatcggcgt catccccctc tcctcgatgg cgggcaataa ggccaccagt
    12181 gggaaaatcc ggcccctgga taaattgcat caggtcggcg agcgagatgt catcgagctt
    12241 ggcccagttt tcgagcatgt agatcagcgc cccgacgacc tcggtcaagt tgtgcggggg
    12301 gatgctggtc gccatgccta ccgcaatgcc cgaagcccca ttaaccaaca ggtttgggat
    12361 ggtagcgggc atgacgcttg gctctttgag ggaatcatcg aagttcgact ggaagtctat
    12421 cgtgttcttg tcgatatctt ccagcaagtc gtagcccatg cgagacatgc gagcttccgt
    12481 atagcgcatg gctgcggccg cgtcgccgtc aatcgagccg aagtttccct ggccatcgac
    12541 aagcatgtag cgcatggaga agtcctgcgc catgcgaacc atcgcttcat agaccgattg
    12601 atccccatgg gggtggtact tgccgagaac ctcgccaaca acacgagccg acttcctgta
    12661 aggggtgtcg gggcgcagcc ccatatcata catggcgtac aggatgcggc gctggacggg
    12721 tttaaggccg tcccgagcat ccgggagagc gcgcgagacg atgacgctca tcgcatagct
    12781 gagataggat tcgcgcatct cctggtctat atttacttgt tttatcagtc cgacttccat
    12841 atggtggcac aataattagg tcgcaaaagc aacgacccac tgaaaatttg ttctatatcc
    12901 gccacaagtg taggcaattt atgggctgat gtcaacgtac aacagcgacg agaggtgact
    12961 aggctccccc gagccaaacg at
//
LOCUS       DAOMQG010000060        12856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2096_length_12856_cov_10.735411, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000060 DAOMQG010000000
VERSION     DAOMQG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_12856_cov_10.735411"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLC06_05295"
     CDS             <1..963
                     /locus_tag="PLC06_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRO89893.1"
                     /translation="YSFVGLAGVSIVVNLITLVLLAILAARRFDLHGPWTLDGTMIRG
                     MVRLGFPLMLIHLLQTIFISIDVLLLRQLLPDGEKVVGWYNVAWKWFNALQIIPSYFT
                     LALFPIVSRAIKENMESARRMYRMSLKLMLLLALPIAALTTFAAPALVGLLGGSEFLP
                     QGAVALQVVIWSIPFGWLNSVTNYVLIALGMERVQPRAFAIAVGFNVVFNWLLIPRYS
                     FVAAGIITVLSELVLLAIFAYFLRRRGAGIDWLRLIARPVFLTLAMLGVMWLGNQLHL
                     IAALVLGVAVYAIGLVVLHIIEPREREAIKAILPGALTRRLGWV"
     gene            966..2975
                     /locus_tag="PLC06_05300"
     CDS             966..2975
                     /locus_tag="PLC06_05300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89894.1"
                     /translation="MRHSQSGEQLTVEHSAGYAPERLALVLMVAILLAAAAFRFIGLD
                     NFPTPQGATPPGLEHDEVAHWLINQDILAGQHAIYFTEAYGHEALYHYVQAAFGAMVG
                     DHALALRLPSAYFGILLVAVAYALGRRLFGVRVALWSAAFLAVLFWPVFYSRLALRAI
                     SLPVFSGLAAYWWWRAFPIGAEPDNDPESNDRTAATSLGMAGLFAGLSIHTYMAARAV
                     PIFFALYSFYLLVWHRPIFRRHWRGLALFWLTFIVVAAPLAIFLLSNPGAEVRVAEVD
                     APLRAFLSGDFRPVLENAARIVGMFGVKGDPLWRQNVAGRPVFDPVMGLLFYAGVALS
                     LWRWRDPRHAFMLLWLATSVIPSLVTVDAPSNIRIINLLPVLMLFPLVFIHNTLQLST
                     IREKLSTDLATSLANTRLVTVGLGFLLVWSAWSTYSDLWRVWPANDEVRFVWQAALTE
                     AAAYLDHSDESGAVAVGGWTPETMDPPTMALSLRRDDLALRYFDPTQSLIIPASADDQ
                     PIRIVKPSILPLATPLQGLVKPWEEVHGEFTLYRMPDQPAVEPRIAADVDFGGQLVFM
                     GNEVVAGCEPGTPCEIMTYWRVIAPSKEPLRFFLHAVDAAGQVVAQDDRLGAPAEYWR
                     EGDLIVQLLALDATAAELRLGVYNPLTRQRLLTRTGADYIALPGN"
     gene            complement(3001..4302)
                     /gene="aspS"
                     /locus_tag="PLC06_05305"
     CDS             complement(3001..4302)
                     /gene="aspS"
                     /locus_tag="PLC06_05305"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRO89895.1"
                     /translation="MTEIRTIEAKNHIGEAVQLKGWLHNVRRLGGVNFIVLRDGWGTI
                     QAVTEDDADLAPIADLALGTVIRLEGLAIANAQAPNGLELQRPRIEVITPVTETPPLP
                     LNKRRLNAALPTLLDHAVVANRHPARRAIFRLSSGVMAGFRATLGAMYFTEVQTPKIV
                     ASATESGANVFRLDYFGRPAYLAQSPQFYKQIMVGVFERVFEVGPVFRAEPHDTVRHI
                     NEYVSLDVEFGFIENHFTVMRLLREVIAGIFTYLIDNYPDDLALLEANLPIILPEIPH
                     IHFADAQELILSRHGVDVRGEPDLSPQDERWLGEWALETHGSDFLFVTGYPMVKRPFY
                     THPDPTRPDYSNSFDLLFRGTELVTGGQRLHRYQDYLAALDRAGYPHEPFETYLEAFR
                     YGMPPHGGFAIGLERLLMQLLGLPNLRLATLFPRDLHRVTP"
     gene            complement(4360..4833)
                     /gene="greA"
                     /locus_tag="PLC06_05310"
     CDS             complement(4360..4833)
                     /gene="greA"
                     /locus_tag="PLC06_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRO89896.1"
                     /translation="MIEDDVLYVTSEGLQQMKDELHNLKTTRREEVALKLEIAIKQGD
                     LKENADYHDAKEEQGFIEARIRQLETMILQAKVIENKGPAGVVRIGSTVTVSEDGYDE
                     METYTIVGAAEADPTEGRISNESPIGSALLGARKGQTVSAQTPAGQIAFKIHDIA"
     gene            complement(5089..6633)
                     /locus_tag="PLC06_05315"
     CDS             complement(5089..6633)
                     /locus_tag="PLC06_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO89897.1"
                     /translation="MNKNNTQRVDEAPIVLEARGITKQFPGVLANDRIDLTLRKGEIL
                     ALLGENGAGKSTLMNILYGLYRPDAGEIWIKGEKVTLHSPGDAIERGIGMVHQHFQLV
                     PVMTVAENVILGAEVTRTGGYIDKRAANRRVHELSEQYGLEIDPTVEVDDLPVGLQQR
                     VEIIKALYRHADILILDEPTAVLTPQESNELFRIMHDLTEKGVSIIFITHKLKEVLAV
                     ADRIVVLRGGRSVGAALPAESTEASLAELMVGRKVILQVDKDEAHPGKKVLEIKNLKV
                     LDDRKQLVVKDLSLEVHAGEILGVAGVQGNGQREFVEALTGLRSIVSGNIEIAGEDST
                     QFTPRAITELDVAHIPEDREKHGLVMPYSIADNMVLNRYYHKPFSNNAILNRQAIAEN
                     GTELVKKFDVRTPSIYTHAGNLSGGNKQKVIVAREFSRPVKLLIANQPTRGIDVGSIE
                     FIHNQIVAQRDAGVAVLLVSAELDEILSLADRVAVMFDGHIVKTLPIEEATRERVGLL
                     MAGSEA"
     gene            complement(6679..7695)
                     /locus_tag="PLC06_05320"
     CDS             complement(6679..7695)
                     /locus_tag="PLC06_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89898.1"
                     /translation="MELTASRRNGSISWLVKNKWLTLIAIIVTGSIIFAYATNDVVTI
                     AVLARTIRESTPLILGALCGLLGERSGVVNIGIEGQMLIAAFIAFLANVYTGNLVLAI
                     IIGALAGALLGAFLAFMAVTLKMDQIIGGTIINILAVGLTGYFYVAGLTTRGKLQPIS
                     LGPLADIPLIGPVLFKNPPITYLAIILVFVVHYVLFYTRWGLRTRAVGEHPSAADTVG
                     INVYKVRYINVILGGAIAGLGGAYLTLEGVGSFERMMTNGRGFLALAILIFGKWTPFG
                     AWGAALLFGFTYALQTQLQFWGVNLPPQFIGSMPYILTILVLAGFVGRSRAPAASGVP
                     YEKG"
     gene            complement(7785..8903)
                     /locus_tag="PLC06_05325"
     CDS             complement(7785..8903)
                     /locus_tag="PLC06_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO89899.1"
                     /translation="MTTIPTTNPDEDRKKKWLRQIWHGISIPVVAVFLGLLIGAIILL
                     ISGASPIEAYSALLKGSFGSPAAIQRTLEKTTPLIFSGLAVAFAFKAGLFNIGAQGQL
                     LIGAIVAAFVGYSIEGLPPLIHIPLALAAGGLAGALYGAIPGILKAYTGAHEVIVTIM
                     LNYVAYNITNYLVNGPWKDPSPTNVIARTARILPSAELPIINHIPLGFIIAVLTAALV
                     WWLIYRTTLGFELRTVGLNSSAAQYAGIRVARMIILAMVISGLLAGFGGAVETLGIVR
                     RYEPGFNVGLGFDGITVALLGKTSPFGVIPAALLLGAMRAGSNVMQLEAHVAKEIIDI
                     ITAVILFFVAADVITRRIIRQRTADDDQVTLSTGWSGK"
     gene            complement(9014..10129)
                     /locus_tag="PLC06_05330"
     CDS             complement(9014..10129)
                     /locus_tag="PLC06_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRO89900.1"
                     /translation="MKRIALLLTGLLLLSLLAVACGGGAATPPAQETAPTEAPAEEAA
                     PTEAPAEEAAGDCTDEAVLCIGLVTDVGEVDDKSFNQSAWEGAQQAAEALGAAEVNFI
                     ETSAATDYGANIALFADQGYDVIVTVGFAMGEATLAAAATYPDIDFIGVDQFQPETPV
                     ANVAGLVFNEDKAGYLAGVLAGSLSQTGTIAAVLGTDLVPAVRAFNIGYQNGALSVNP
                     DIKVIATYHPGGFESGFTSPEDGAATAQQAIANGADFVFGAGGKTGNGAIIEAASHEG
                     VWCIGVDSDQWGTVPEAHPCLITSAMKLITPGVVDLITKSVNGEFPGGNYYGLVGLAP
                     FHDHEDAVPQEVRDLLATTDAGLQDGSISTGFDPATD"
     gene            10467..11918
                     /locus_tag="PLC06_05335"
     CDS             10467..11918
                     /locus_tag="PLC06_05335"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89901.1"
                     /translation="MDPMRLLDILDAHLSEAEMSALCRQFGVAFGAFPGGAKRDKMRE
                     FLGFIQRQGRMGGLTEATIALRPDLTEAIAGLYEGKEQELSWLDQVAGGIGQSLDSGL
                     TWRWTTSSTGRQVAVGSSSAADEETTPDVVLAVEDSSPSRDVDNPYTPGARVIDEAMF
                     FGREVETEQVSRLLEDSRHVAIIGHRGFGASSLLYHVSHALGDRDKTLVAYVDMKDAV
                     NHTPASLLNAIWLQWWERVRPGNSVPVRNLAEFVTAVRKLNAAGFHPVLFVDELEQLA
                     WRSASFDDGFFAAWHELGREGLVQFVVAAHSTPADILAQADLKSSFYELFQSVNLGLL
                     DESAALSLLAIPIRRAGMSVPDGAAEYLYLHAGPHPFFLHLAGLYLFDAIAGGNYSRG
                     EVVRQFEIAAEPFWQELWDSISPLAQSHYPTTLIRVSDGMGGRQMRILANRGLVVVEE
                     GGFRPFSDGFARWLARMQAAIEAAAVVADAIPL"
     gene            12021..>12856
                     /locus_tag="PLC06_05340"
     CDS             12021..>12856
                     /locus_tag="PLC06_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HRO89902.1"
                     /translation="MKRAVSISLGSSKRNSSVEIELLGERVSIERIGTDGDMTRAAEM
                     FGELDGKVDAFGVGGTDLGLTIDDRHYRMHSVMSLVRYVKQTPIVDGSGLKNTLERGV
                     VPYMEAQIGPPQPRRVLMTSGADRWGMSMSFLENGYEGVFGDLMFALSLPIPLRSEAA
                     IKRAATLLFPIVTRLPFEWIYPTGEKQEVNTPKFGEWYEWASVIAGDCHFIKRFMPAR
                     LDGKIICTNTTTPEDVAIFRNAGASYLVTTTPRLEGRSFGTNMMEATLVAVSGEGRKL
                     TDA"
BASE COUNT         2551 a         3562 c         3784 g         2959 t
ORIGIN      
        1 tacagtttcg tggggctggc cggggtgtcg atcgtcgtta atctgatcac gctggttttg
       61 ttggccatcc tggcggcgcg gcgatttgat ctccacggtc catggacact tgacggcaca
      121 atgatccgcg gaatggtgcg gctcggcttc ccgctgatgt tgatccatct cctgcaaacg
      181 atcttcatct ccatcgacgt cttgttgctc agacaactgt tgccggatgg tgagaaggtc
      241 gtcggctggt acaacgtcgc ctggaaatgg ttcaacgcgc tacagatcat cccgtcctac
      301 ttcaccttgg ctttgttccc catcgtttcg cgggcgatca aggagaatat ggagtcggcg
      361 cggcgcatgt accggatgtc gctcaagctc atgctgttgc tggccttacc gattgctgcg
      421 ctgacgacat tcgccgcgcc cgcactggtc ggcttgctcg gcggctcgga gttcctgcct
      481 cagggcgcgg tagccctgca agtcgtcatc tggtcgatcc cgtttggctg gctcaatagc
      541 gtcaccaatt atgtgctgat cgccctgggc atggagcgcg tgcagccgcg ggcattcgca
      601 atcgccgtcg ggtttaacgt cgtgttcaat tggctgctca tccctcgcta tagctttgtc
      661 gctgccggca tcatcaccgt cctgtccgag ttggtcttgc tggcgatatt cgcctacttc
      721 ctgcgccggc gcggcgcggg aatcgattgg ctccgtctca tcgcccgtcc cgtgttcctg
      781 acccttgcca tgctcggtgt catgtggctg ggcaatcaat tgcatttgat cgccgcgctc
      841 gtgttgggcg ttgcggttta tgcgatcggg ctggtcgtat tgcatatcat tgagccaaga
      901 gaacgcgagg ccatcaaggc gatattgccc ggtgcgctca ctcgccggct ggggtgggtt
      961 tgacgatgcg ccacagccag tctggtgagc aattaaccgt cgagcattct gcgggatatg
     1021 cgcctgaacg attggcgttg gttctcatgg tggccatcct gcttgccgcc gccgctttta
     1081 gatttattgg cctcgataat tttcctactc cacaaggcgc gacgcccccg ggcctggaac
     1141 atgatgaggt cgcccactgg ctgatcaatc aggacattct ggccgggcag cacgcgatct
     1201 acttcaccga ggcctacggc cacgaagcgc tctatcatta cgtgcaggcg gctttcgggg
     1261 cgatggtcgg cgatcatgcg ctggccctgc gtctgccgtc ggcctatttc ggcatcctgt
     1321 tggtggctgt tgcctatgcg cttgggcgcc ggctgttcgg cgtacgtgtg gcgttgtggt
     1381 cggccgcatt cctggcggtg cttttctggc cagtgttcta tagccgcctg gcgttgcggg
     1441 ccatctcact gccggtattt tccggtttgg cggcttattg gtggtggcgg gccttcccca
     1501 ttggcgcaga gcctgacaat gatccagaga gcaatgatag aacggccgca acaagcttgg
     1561 gtatggccgg tttgtttgcc ggattgagca tccacaccta catggcggcg cgggccgtgc
     1621 cgatattctt cgctctatat tccttttatt tgctcgtatg gcatcgacct attttccgcc
     1681 gtcattggcg cgggctggcc ttgttctggc taacgtttat tgtggtcgct gcgcccctgg
     1741 ctatttttct gctgtcgaat cccggcgcag aggtacgcgt cgctgaagtt gacgcgccac
     1801 tgcgagcgtt tttgtcggga gattttcggc cggtgctgga gaacgcggcc cgcatcgtgg
     1861 gcatgtttgg cgtcaagggc gatccgcttt ggcgacagaa cgtcgccggg cgaccggtct
     1921 ttgaccccgt catggggctt cttttctatg ccggcgtcgc actcagtctt tggcggtggc
     1981 gtgacccgcg ccatgccttc atgttattat ggctggcgac gtcggtgatc cccagcctgg
     2041 tcaccgtcga cgcgcccagc aacatccgca ttattaacct gctaccggtt ttgatgctgt
     2101 ttccactcgt atttatccac aacaccctcc aattatccac aatacgggaa aagttatcca
     2161 cagatttagc cacaagcctg gcgaatacaa gacttgtaac cgtggggtta ggcttcttgc
     2221 ttgtctggtc ggcatggagc acgtactccg atctgtggcg cgtgtggccg gcgaatgatg
     2281 aggtgcgttt tgtatggcag gccgccctca ccgaggcggc cgcgtatctg gatcatagcg
     2341 atgagtcggg ggcggtagct gtcggcggtt ggacgccgga gacgatggat ccgccgacga
     2401 tggcgctaag tctgcggcgc gacgacctgg cgctgcgcta cttcgatccg acgcaaagcc
     2461 tcattatccc cgcttcggct gacgatcagc ccattcgcat agtgaaaccg agtatcttgc
     2521 cactggccac acctcttcag ggcttggtga aaccgtggga agaagtccac ggcgaattca
     2581 ctctctacag aatgcccgac caaccggccg tcgaaccgcg gattgcggcc gacgtcgatt
     2641 ttggtgggca acttgtcttc atgggtaacg aagtggtggc gggatgcgaa cccggaactc
     2701 cgtgcgagat aatgacctac tggcgtgtca ttgctccatc caaagaaccg ctgcgattct
     2761 ttcttcacgc ggtggatgcg gcagggcagg tggtggcgca agatgatcgt ctcggagcgc
     2821 cggccgagta ttggcgagag ggagatctca tcgtgcagct tctcgcgttg gacgccacgg
     2881 ccgcggaact tcggttaggt gtctacaacc ccctcacccg tcaacgcctt ctaacccgga
     2941 cgggggccga ttatatagcc ctgcctggaa attaaccggc gaggcggaat gggcttgtgg
     3001 ttatggcgtt acgcgatgga gatcgcgcgg gaaaagggtc gccagccgca ggttgggcaa
     3061 acccagcagc tgcatcaaga gacgctccag gccgatggcg aagccgccgt ggggaggcat
     3121 gccatagcga aaagcttcga ggtaagtttc gaatggctca tggggataac cggcccggtc
     3181 gagcgcggcc agatagtcct ggtaacggtg caagcgctga ccgccggtca ccagctcagt
     3241 cccgcgaaac agcaggtcga aggagttgga ataatccggt cgcgtcgggt cagggtgggt
     3301 gtagaacggc cgcttgacca ttggatagcc ggtcacaaac aggaagtcgc tgccatgtgt
     3361 ctctaacgcc cattcgccca accagcgctc gtcctgtggc gacagatcag gctcaccgcg
     3421 cacatcgacc ccatgacgac tcaggattag ctcctgggca tcggcaaagt ggatgtgggg
     3481 tatctccggc agaatgatgg gcaggttggc ttcaagcaag gccaaatcat ccggatagtt
     3541 gtcgatcaga taggtgaaga tgccggcgat cacctcgcgg agcaggcgca tcaccgtgaa
     3601 atgattttcg atgaagccga actcgacatc caggctgacg tattcgttga tgtgacgaac
     3661 ggtatcatga ggttcggcgc ggaagaccgg gccgacttcg aacacgcgct cgaagacgcc
     3721 gaccatgatc tgcttgtaga actggggact ttgggccaga taagcggggc ggccgaagta
     3781 atccagcctg aacacgttgg ctccgctctc tgtggccgag gcgacgattt tgggcgtctg
     3841 cacttcggtg aagtacatgg cccccagcgt cgcgcgaaac ccggccataa cgccggatga
     3901 cagcctgaat atggcccgtc gcgccgggtg acgattggcg acgacggcgt gatcgagcag
     3961 tgtgggcagg gcggcgttca gccggcgttt gttgagcggc agcggtggcg tttcagttac
     4021 cggtgttatg acttcgatcc gcggccgttg cagttcgagg ccattaggtg cctgcgcatt
     4081 ggcaatggcc aacccttcaa ggcggatgac cgttcccaga gcgagatcgg cgataggtgc
     4141 aaggtccgca tcatcttcag tgacggcctg gatggttccc cagccgtcgc gcaggacgat
     4201 gaaattgacg ccgcccaacc ggcggacgtt atggagccag cctttgagtt ggacagcttc
     4261 accgatgtgg ttctttgctt caatcgtgcg gatctcggtc atgatgttgc tcaatctgaa
     4321 gacggccatt agtcttgccg gccctgtcaa ccccgtttgt caggcgatgt cgtggatttt
     4381 aaaggcaatt tgtcccgcgg gggtctgggc gctgacggtt tggcccttcc tggcccccag
     4441 cagcgcgctg ccgatcggcg actcgttgga gatgcgtcct tctgtcggat cagcctcggc
     4501 cgcgccgacg atagtgtaag tctccatctc gtcatacccg tcctcggata cggtgacggt
     4561 gctgccgatg cgcacgacac ccgccggccc cttgttctcg atcaccttgg cctgtagtat
     4621 catggtctcc agttggcgaa tgcgagcctc gataaagccc tgttcttcct tggcgtcgtg
     4681 atagtccgcg ttttctttca ggtcgccttg cttgatggca atttccagct tcagcgctac
     4741 ttcttcgcgg cgagttgtct tgaggttgtg caactcatct ttcatctgtt gcagcccttc
     4801 ggatgttacg tacagaacgt cgtcttcaat catggtatcc ttctccgcgt ctttttcagg
     4861 tcgctacctg gcattgtcga tacaaaagaa aagcccagcc gatcaatgcg gctgggcgac
     4921 ttacccacta tttgcttgtt ggcgaaatgg gttagcaatc gctcgatgcc gcgggcgtat
     4981 cgttcgccgc cggcgggggg aaattcagaa cgttcagatc tcgtgtcacg ttgttcatag
     5041 cgtgtcgaaa gtatagcgca acaagccggt attgtctacg actcagcttc aggcttcgga
     5101 gccggccatc agcaaaccga cgcgttcccg ggttgcttct tcgataggca gcgtcttgac
     5161 gatatggcca tcgaacatga cggccacccg gtcagccagg ctaaggatct cgtccagttc
     5221 ggccgatacc agaagaacgg ccacgcccgc gtcgcgctgg gccacaatct ggttatggat
     5281 gaactcgatg gaaccgacgt cgatgccgcg ggtaggctgg ttggcgatga gcagtttcac
     5341 cggccgtgaa aattcccggg cgacaattac cttttgcttg ttaccacctg acaggttccc
     5401 ggcgtgggtg tagatcgacg gggtgcgcac atcaaacttc ttgaccaact ccgtgccatt
     5461 ctccgcgatt gcctgccggt tcaggatcgc gttattagag aatggtttgt gataatagcg
     5521 attgagaacc atgttgtcgg cgatggagta gggcatgacc agcccgtgtt tttcgcggtc
     5581 ttcagggata tgggcgacat ccagctcggt gatggcccgt ggggtgaact gggtggagtc
     5641 ttcgcccgcg atctctatgt tgccgctgac gatcgaccgc aacccggtca gagcttctac
     5701 gaactcgcgc tggccgttgc cctgaacacc ggctactccg aggatctcac cggcatgcac
     5761 ttcaagcgac aggtctttga cgacaagttg ctttcggtcg tccagaactt tcaggttctt
     5821 aatctccagg acctttttgc cgggatgggc ctcatccttg tccacctgaa ggatgacctt
     5881 gcggccgacc atcaactcgg ccagactggc ttccgtcgat tcggccggta gggcggcacc
     5941 tacgctgcgg ccgccacgca ggacgacaat tcgatcggcc acggccagca cctctttcag
     6001 cttatgggtg ataaagatga tcgagacacc tttctccgtc aggtcgtgca tgatgcggaa
     6061 cagttcattc gattcctgcg gtgtcagcac ggccgtcggc tcatcgagaa tgagaatgtc
     6121 ggcatgacgg tagagcgctt taatgatctc tacgcgctgt tgcaggccaa ccgggagatc
     6181 atctacctcg acagtgggat caatttccag cccatattgc tccgacaact cgtgcacgcg
     6241 acgattggcg gctcgcttgt cgatgtaacc cccggttctg gtcacttccg cacccaggat
     6301 aacgttttcg gccacggtca tgacgggcac gagctggaaa tgctggtgaa ccatgccaat
     6361 gccacgctcg atcgcgtcac ctggactgtg cagtgtcact ttctcccctt tgatccagat
     6421 ctcacccgcg tcggggcgat agagaccgta gaggatattc atcagtgtgc tcttgcccgc
     6481 gccgttttcg ccgagcaggg ccaatatctc gcctttacgc aaggtcagat cgattcgatc
     6541 gttggcgagg acgccgggga actgtttggt gatgccgcgc gcttccagca cgatgggagc
     6601 ttcgtcgaca cgttgggtgt tatttttgtt cattcaatgg cctttcccat gcctgttaat
     6661 gagcttgacg atgcgcgatt agcctttctc gtagggaacg ccactggcgg ccggcgcgcg
     6721 agatcggccc acaaacccgg ccaggacgag gatcgtcaga atatagggca tgctaccgat
     6781 gaattgcggc ggcagattca ccccccagaa ctgaagctgg gtctgcaaag cataggtaaa
     6841 gccaaacagc aaggccgcgc cccatgctcc aaagggtgtc catttgccga agatgaggat
     6901 cgccaacgct aggaagccgc gaccattggt catcattcgc tcgaaggatc cgacgccctc
     6961 cagagtaagg tatgctccac ccagcccggc aatagctccg ccgaggatga cgttgatgta
     7021 gcgaaccttg tagacgttga tgcctaccgt gtcggccgcg ctgggatgtt cgccgaccgc
     7081 gcgagttcgc aacccccacc gtgtatagaa caagacgtaa tggacaacga atacgagaat
     7141 gattgccaga taggtgatcg gcggattctt gaacaggacc gggccaataa gtggaatatc
     7201 agccagcggg ccgagagaga tcggctgtaa cttcccgcga gttgtcaggc cggctacgta
     7261 aaagtagcct gttaacccaa cggccaggat gttgataatc gtaccaccga taatttggtc
     7321 cattttcagc gtgacggcca taaaggctaa aaatgcgcct aataacgcgc cggccagtgc
     7381 gccgatgatt atcgccagta ccaagttgcc ggtatagaca ttggccagga aggctatgaa
     7441 cgcggcgatg agcatctgcc cctcgatacc aatgtttaca acgccgctcc tctcgcccag
     7501 caagccgcat agcgcaccca ggatgagagg agtcgactcg cgaattgtcc tggctaacac
     7561 ggctatcgtc actacatcgt ttgtcgcgta ggcaaaaata atggatccag tgacaataat
     7621 ggcaatcagg gtcagccatt tattttttac cagccagctg attgatccat ttcggcgcga
     7681 tgcagtcagt tccatattgt tccctctcaa acaatgatga atcaggattt aaggattgga
     7741 ttaccaatct ggatccttaa tctgtaattc ttaatctcgt ttccttactt cccgctccac
     7801 cctgtgctta gtgtcacttg atcatcatca gccgtgcgct gtcgaatgat ccgtctggtg
     7861 atcacgtcgg ccgcgacgaa gaacaaaatt actgcggtta tgatatcgat tatctctttg
     7921 gctacatggg cctcgagctg cattacattg gagccggcgc gcatggctcc cagcagcaag
     7981 gcggcaggta tgacgccaaa tgggctggtt ttgcctaata aagccacggt gattccgtcg
     8041 aagccgaggc caacgttaaa tccaggctca taacgacgaa cgatgcctaa tgtttcaacg
     8101 gcgccgccga acccggccaa taaaccgctg attaccatag ccaggatgat cattcgcgcg
     8161 actcggattc cagcatattg ggcggcactg gagttcaggc cgacggtgcg gagctcaaac
     8221 cccaatgtgg tgcgataaat taaccaccag acgagcgccg cggtaaggac ggcgattata
     8281 aagcccagag gtatatgatt gataatcgga agttcggctg atggcaagat tcttgccgtg
     8341 cgggcgatga cattggttgg cgatggatcc ttccacggtc cgttgaccag atagttcgta
     8401 atgttgtagg ctacatagtt gagcatgatg gttacgatga cctcatgcgc cccagtgtat
     8461 gccttcaata tacctggaat tgcaccatag agcgcgccag ccagtccacc ggctgccagg
     8521 gcgagtggaa tgtgaatgag agggggcaac ccttcgatcg aatacccgac gaaggccgct
     8581 acaatggctc caattaataa ttgcccttgt gcaccgatat taaaaaggcc ggctttgaag
     8641 gcgaaggcga cagcaagccc actaaatata agcggagttg tcttttcaag tgttctctga
     8701 atggccgcag gagagccgaa cgagcctttc aagagtgcgc tgtaagcttc aataggactt
     8761 gcgccggaaa tcagaaggat tatagcccca ataagaagcc ccaagaagac ggcgaccacc
     8821 ggtatgctga taccgtgcca aatctgtcgc aaccactttt ttttccgatc ttcgtcggga
     8881 ttagttgtcg gaattgtcgt caacgccacc tcctagacaa tgtgcgacgt gccgtatcgg
     8941 gcacgtcgca cattgaaaga ctaaataagc attagatttt caatagatct aagctcgcag
     9001 ctttatcttc cgtctagtct gtggcgggat caaaccccgt tgaaattgaa ccgtcctgca
     9061 aaccggcgtc agtcgtggcc aatagatccc taacttcttg tggaacggcg tcttcgtgat
     9121 catggaaagg cgcaagaccg accagcccat agtaattccc gcccgggaac tcgccgttta
     9181 ccgacttcgt aatcaaatcg acgaccccag gggtgatgag cttcatcgca ctcgttatga
     9241 ggcaaggatg cgcttcgggc acggttcccc actgatcgga gtcgacgccg atgcaccata
     9301 cgccttcgtg ggacgctgct tctatgatcg cgccgttgcc tgtcttgccg ccggcaccga
     9361 aaacgaagtc cgcgccgttg gcgattgctt gttgggcagt tgcggcaccg tcctcaggag
     9421 aggtgaaacc gctctcgaaa ccaccgggat ggtaggtggc gataaccttg atgtcggggt
     9481 taactgacag ggcgccattc tggtaaccaa tgttgaaggc tcttacagcc ggaaccagat
     9541 cggtacccag cacagcagcg atggtgccgg tctggctgag agacccggcc agtacaccag
     9601 ccaaatatcc cgccttgtct tcgttgaaga ccaggccagc gacgttcgcg acaggtgttt
     9661 cgggttggaa ctgatccacg ccgataaagt cgatgtcagg atatgtggcg gccgcggcca
     9721 aggtagcctc gcccatggcg aaccctacgg tcacgatgac gtcgtaaccc tgatcggcga
     9781 acagggcgat gttggcgccg tagtcggtgg cagcggaggt ctcaatgaag ttaacttcgg
     9841 ccgcgccgag cgcttcggcg gcctgctgcg cgccttccca agctgactgg ttgaaggatt
     9901 tgtcgtctac ttcacccacg tccgtcacga ggccaatgca aagcacggct tcatcggtgc
     9961 aatcgccggc agcttcctcg gccggtgctt cggtcggggc ggcctcctcg gccggtgctt
    10021 cggtcggggc agtttcctgc gccggcggcg tggcggctcc gccaccgcaa gcaacagcaa
    10081 gcagtgacaa caagagcaac ccggtgagca acagagctat acgtttcata attctttctc
    10141 ttctccttgt gaaacagaaa gtaaagggta agctacggtc actgatggtt taataaagcg
    10201 atcccggaaa ttaaaggtca caggttgaag accacacact gcggcgaacc gcggactctt
    10261 tatcttccca ccctggtgcc cgcacctctt catgtattgt gtctcaacat gcaggaaaag
    10321 acaatatttt gttgagatcc attttcgatg ttgagatcaa gcgtatcggt tcgctttgtt
    10381 gaccctttcg taaaaataag ttcgtcgcgg gcgttgtagt tggggagaaa tacacattat
    10441 cgttatacta tcgctcggaa atctttatgg acccgatgcg attgctcgat atcctggatg
    10501 ctcatctttc cgaagcggaa atgagcgcgc tgtgccgcca gtttggggtg gcatttggcg
    10561 cgtttccggg cggtgcaaag cgagacaaga tgcgggaatt cctcgggttt atccagcgac
    10621 agggacgcat gggtgggctg accgaagcaa cgattgcctt gcgccccgat ctgacagaag
    10681 ccattgccgg gctgtacgag ggcaaagagc aggaactctc ctggctcgat caggttgccg
    10741 gtggcattgg acaatcgctt gattcggggc tgacgtggcg atggacgacg tcttcaaccg
    10801 gccgtcaggt tgccgtcggt tcatcatcag ctgccgatga ggaaaccact cctgacgtgg
    10861 tcttggcggt ggaagattcg tcgccatctc gtgacgtcga caatccttat acacccggcg
    10921 cgcgtgtgat cgacgaagct atgttctttg gccgcgaggt agagacggag caggtcagtc
    10981 gtctgcttga ggacagccgc catgtcgcga tcatcggcca tcgcggcttt ggggcctcct
    11041 cgcttctcta tcacgtttcg catgcgcttg gcgatcgaga caagaccctc gttgcctatg
    11101 tcgatatgaa ggacgccgtc aaccacactc cggcaagcct tctcaatgcg atctggctgc
    11161 aatggtggga gcgcgtgcgg ccgggcaaca gtgtgcctgt gcgcaacctg gccgaattcg
    11221 tgacggctgt ccgcaagctg aacgccgccg gattccatcc cgtgttgttt gtggatgagc
    11281 ttgagcagtt ggcatggcgg tcggcgagct tcgatgacgg cttctttgcc gcctggcacg
    11341 agttggggcg cgaggggttg gtacaattcg tagtcgctgc ccactccacc ccggccgaca
    11401 tcctggctca ggcggatttg aaatcctcct tttatgagct tttccaatcg gtgaacctgg
    11461 gattgctgga tgaatcggcc gctctttctc tgttggcgat ccccatccgg cgcgccggga
    11521 tgagcgtacc cgatggtgcg gcggagtacc tctacctcca tgcggggccg caccccttct
    11581 tcctgcatct ggccggtctc tatctcttcg atgcgatagc cggtggaaat tattcgcgtg
    11641 gggaggtggt tcggcaattt gagatcgccg ctgagccgtt ctggcaagag ttgtgggact
    11701 ctatctcgcc tctggcccaa tcccattatc ccactacctt gatacgggtg tctgacggca
    11761 tgggcggccg gcaaatgcgc atactggcca atcgtggttt ggtggtggtg gaagagggcg
    11821 ggttccgacc atttagcgat ggattcgcgc gttggttggc gcgcatgcag gccgctatcg
    11881 aagcggccgc ggtggtggcc gacgccatac cgctctgatc agatccacta aggcgacggt
    11941 ttgtactgat taccccaatt gagcgagttg ggtttgaaca gtgatggaat agattcattc
    12001 atgcagcagg acggcgatca atgaaacgag cagtcagcat cagtctggga tcttccaaac
    12061 ggaattcatc ggtcgagatt gaattattgg gcgagcgtgt atcgatcgag cgcatcggca
    12121 cggatggtga tatgacgcgc gcggccgaga tgtttgggga attggacggc aaggttgacg
    12181 ccttcggcgt tggcggcacg gacttgggac tgactatcga cgacagacac tatcgtatgc
    12241 actctgttat gtcgctggtg cgttatgtca aacaaacacc gatagtcgac ggttcgggcc
    12301 ttaagaacac gctggaacgc ggcgtcgtac cctatatgga ggcacagatt gggccgccac
    12361 aacctcggcg ggttctgatg accagtggcg cggatcggtg gggcatgtcg atgtcgttcc
    12421 tggagaatgg ctacgaaggc gtgttcggcg atctgatgtt cgccctgagc ctgcccatcc
    12481 ccttgcgttc ggaggcggcc atcaagcgcg cggcgacgct cctctttccc atcgtcaccc
    12541 gcttgccctt cgaatggatc tatccgacgg gcgaaaagca ggaggttaat acgccgaaat
    12601 ttggcgaatg gtacgaatgg gcttcggtca tcgccggtga ttgtcatttc atcaagcgct
    12661 tcatgccggc gcggctcgac gggaagatca tttgcaccaa tacgaccacg cctgaggatg
    12721 tggccatctt ccgtaacgca ggcgcaagtt atttggtcac gacgactcca cgtctcgagg
    12781 gacgatcatt tggcacgaac atgatggaag cgacgctcgt cgccgtgagt ggcgaggggc
    12841 gcaagttgac cgatgc
//
LOCUS       DAOMQG010000061        12645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2144_length_12645_cov_9.811994, whole genome shotgun sequence.
ACCESSION   DAOMQG010000061 DAOMQG010000000
VERSION     DAOMQG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2144_length_12645_cov_9.811994"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLC06_05345"
     CDS             complement(<1..965)
                     /locus_tag="PLC06_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRO89903.1"
                     /translation="MTGNKNNAIYVVGHVNPDTDAIASAMGYAWLLQDQGQNAIAARA
                     GHLNPQTTWVLNHLKIEQPTLLIDASPRFSTISHRLNTAAPEHPLREVWSIANRTGGV
                     APIVDSDGKPYGLVTVISLFDFLSRTIGSHPRREEMRIGELMDMPGQEAVDRTVPQFL
                     ASARIRDALNRILREERTEFWVVDETGRYVGICRHREALNPPRMQLILVDHNEPGQAV
                     GSIDEADIIEIVDHHRLGNPSTRIPIRFTVDVVGSTSTLISERINDAGLSAPPELAGL
                     LLAGVLSDTLILTSPTTTPRDHEAAERLGRWAFVIGSPLAGETVQS"
     gene            1284..1841
                     /locus_tag="PLC06_05350"
     CDS             1284..1841
                     /locus_tag="PLC06_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO89904.1"
                     /translation="MIEIHPLEKFSASRLRQIASGYTTNEIYRVEYHEGQVETVFRLT
                     LEPLPESKSFVFPFDEEELKGYEALIPNPFCLGAYDDGLWVGVALAEPRLWNNTLWVW
                     EFHVAETYRGKGIGRRLMERLAASAEAAGLRSLICETQNTNVPAIRFYHAVGYRVEGV
                     DISYYTNEDMLPGRTVAVFMKRRLT"
     gene            1838..4174
                     /locus_tag="PLC06_05355"
     CDS             1838..4174
                     /locus_tag="PLC06_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89905.1"
                     /translation="MNGLIRFVEERPGLSAALLFLGLGLVFFAAVLLPPAGQVLAGHD
                     MIGYYYVYWEAVREALLSGRLPLWEPDTFGGFPFLAQPQQNTFYPINWINLILPLRVG
                     VSLYMVFHVWLAGFGMYVFGRIMGGRWLPSVLAGIGFAFGGLLAGRLWAGHQPVYAVF
                     IWTPLMLAALVWAVNRQSWPSAVVAGVPFALAILAGHIPSFLYVGMIWAAFAAFLFIT
                     RRDARTTVIRAAVIAGIVGLALAAVQLAPFLQFSATSGRVAEADYEFATDYSLPPAHL
                     VTLLVPGFFGEPLRVGYWSVPTFEELTYYAGLVAVLGILLALRRPTKLSWFYLLLIVF
                     GLWLALGRYGILYELVYRFLPPFRLVRAPGRAAFLYLFAASALLAHTLTVWREMPADR
                     RRRMLKPFWPTAVIVLGLALFSALAATGAMFMAVHPTDTSGRLWHQIGGYSLALVVVG
                     TGGALLWAYLTGDQVESQPPGSSNGVSRPHGALSRHTMRFAFIAVALVALATIDTWWF
                     SYPMVRTAPTVPEAIWTDGRQLVGDVEGRVLPWGVPLFSQNGAMRVDWPSVFGYDSLE
                     PAAHIALASSVPDPRSTAYDILGASHVLAGGPLDEFTQGEQGLILLGQQGAAWVYSRP
                     RALPLARLVNQVEIIAEDVAIITRIHEPGFDPAIVAILNAPPPCTLEGGPLGTATVMT
                     HRPTRWQIATSSAGEALLVLAENAYPGWEVRIDGQRAEPLTAYTSIRAVCVPAGEHTV
                     EWVFRPWLYGAGAVITLVALALVIFAAIRLRRPESSVR"
     gene            complement(4193..5386)
                     /locus_tag="PLC06_05360"
     CDS             complement(4193..5386)
                     /locus_tag="PLC06_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRO89906.1"
                     /translation="MQYDLVFEGGGAKGMVFVGALQEFERLGHTAGRLLGTSAGAIAA
                     TYLAAGFTADEMMRALKEKDESGKHVFTRFLGRPKPFTKEEIAHSETRAALEKINLPW
                     MPDFIESRFDRFILSLMTQEELAHIFSFVERGGWYSADAFVEWARTKLDQATPDGEVR
                     RLSNHTFAEFYLKTGRELSLVAADTTAHRLLVLNHRTAPRLPVVWGMRMSMSLPLLWQ
                     EVIWQPEWGNYLSYSMAGHRIVDGGLLSNFPIELFLSSDPYVVSLMGPKTSQGMLGLL
                     IDEELSVPGIDPDPSNEANGSLTDLPPVERIIGLIDTATQAHDKMVIEAFEQFVCRLP
                     AKGYGTTEFDMSDYRRDALITSGKHVMADYFRTRETQGMKGPDVDEMERAFRQADRLA
                     NKILH"
     gene            5577..7610
                     /locus_tag="PLC06_05365"
     CDS             5577..7610
                     /locus_tag="PLC06_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRO89907.1"
                     /translation="MRKPDTFYARLVAVLLLPLLLIGALPAAAQEAPGDFIPSDCMFG
                     GIDLGLTKIGGESLGFECGYVVVPERHANPDGPTIRIPVAVRRATAPNPHPDPLVLAQ
                     GGPGGDAFEIFTLLVPSTDIAANRDIIIFNQRGTPYAEPELSCPETAAVLPQTLAATE
                     EEGNRLYNEALSACFERLQSEGIDLSAYNSLENAADVPLVVQALDYDEYNFYGVSYGT
                     LLGLHLMRNHPEGLRSVILDSVIAPDINFLSEVPASENRVLDEVFAACEADPDCREQY
                     PNLEERFFNLVRQFDDKPVTLQLRDPETGNKYETYMDGAALRSVMFQMLYVSRMSAVM
                     PKVVADLEKGDTRYIENMWPLFVFDQLIAEGMYYSVVCAEDADIDPAAISVDSLRPEI
                     AATARDEIQSYLDTCARWQVELLPPSVDAPVISDIPTLLLSGRFDPVTPPAFAAAVAA
                     GLPNGTALVDPTASHGVAFMTQCANEVVGAFLDDPMNPPDGACFDAQKPLKAVPPNAV
                     TVPLLAGVNSLDSGTISAFVLAGILLVIVLSPVLLWPLVFAARAFGGDPPPQRAPKDR
                     RVRWISRIVVLLFGVLGLIFAVGLIGSIVMVVATDQTLMTALSLPASAAPILWVPVFM
                     LLMAVLMIAAGVMLWRHPGAGTTLGKVYYSIIVIAAVALLGVIGAQGLLLPPL"
     gene            7680..8300
                     /locus_tag="PLC06_05370"
     CDS             7680..8300
                     /locus_tag="PLC06_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89908.1"
                     /translation="MIPKESRFKRMLRAVIFCSAVAALAIFLIDDFKRGTTEPPAARL
                     LPDLPAYRQVEGQTITGYIGALAEGAALLAGHPQLAVTVGTLDQAIDCYQEIGGVRAR
                     VYSHAERPLEAGLVAVADEARLNDPDSLFRCVAPATLNLEGTQEPVIEPCTAAYTMVN
                     EEGTFYIIYAASTYGVCRDFCESLEGCDVHAINSLEFSLRDLRGWL"
     gene            8349..9689
                     /gene="secD"
                     /locus_tag="PLC06_05375"
     CDS             8349..9689
                     /gene="secD"
                     /locus_tag="PLC06_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRO89909.1"
                     /translation="MRSQRDIVWLAFIIILVAGAIWVITNPRFPIRYGLDLQGGLQVL
                     MEADVPADQVVTSDAMDTARQIVDRRVNAIGVTEPLVQVEGNRRILVELPGIEDPEQA
                     LALIQETALLEFVDTGEFSLPEGMCIRTSLNEGPSRCESETVDGEAVATTEEPPTFPT
                     ILTGAGLRDAGVTSDQLGQYFVDFTLTEEGGKVFAEHTRANQGKYLTIVLDKRVVSSP
                     TISAVIEDRGTITGNFTLEEAQHLATQLRFGSLPIPLRIESTRQIGATLGEQSIDASI
                     QAGTIGVAMVLLFMLIYYRLPGVLADVALIVYVLLSFAIFMGVGVTLTLPAITGFLLS
                     TGMAVDANVLVFERIKEEMRRGAHLEDAIDTGFSRAWNSIRDSNVATLVICLILWTFG
                     RNFGASTVEGFAVTLAIGVFISMFTAVLVTRTLVRVFLGRNANRLQGQKWLMGV"
     gene            9712..10647
                     /gene="secF"
                     /locus_tag="PLC06_05380"
     CDS             9712..10647
                     /gene="secF"
                     /locus_tag="PLC06_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRO89910.1"
                     /translation="MSFVYHIVENRRRYFLFSGTLLALGLIAMIASAVMTGSPFRIGV
                     DFRSGTRFEVQFEEPVTENQIRDVFAEYGITSPSIIALRGENLENAWQIRGEFVAPET
                     AQAILNSLGEMAPLRKEASSVTSVSAAVGNEVTRAAIVAVAFSAVVILFYIVVVFRQV
                     PNTFRYGTTAVIALLHDLFIVLGFAAITGLLLGWEIDALFLTAVLTIAGFSLQDTIVL
                     FDRMRENISRRPFEKIDLIANRSIVETIHRSLVTQLNAMFVMVAILLFGGLSVRPFIA
                     TLFFGLLLGTYSSIFIAVPLLVTWEERISTRGRAA"
     gene            10655..11656
                     /locus_tag="PLC06_05385"
     CDS             10655..11656
                     /locus_tag="PLC06_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRO89911.1"
                     /translation="MQALVLRDGQLAYHADWPQPIPGPEEALIRVSLAGICSTDLELV
                     KGYAGGFNGILGHEFVGVVEQVGAQAPTEWIGQRVVGSINIGCGQCLVCQSDGPEHCP
                     DRTTLGIHHRDGVFADRVVLPVANLFSVPDVVSDEEAVFTEPLAAAVRVREQVHIRPT
                     QRAAVVGPGRLGLMVAQVLALAGGDVIVLGRRPESLELPKRLGLSVGLVDELSDNSMD
                     LVIEATGNDAGFAHSLRLVRPLGTLVLKSTFHGNANINLTKLVVGEINVVGSRCGPFG
                     PALRLLEANARAAGSLMAGAGVQVRPLVDAEYALRDGLAAFEYAARPGVHKVLLRPN"
     gene            complement(11653..>12645)
                     /locus_tag="PLC06_05390"
     CDS             complement(11653..>12645)
                     /locus_tag="PLC06_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRO89912.1"
                     /translation="GAIKLARQIQMARGEEKRNIVLSRWQSYHGMTLGALAVSGRAGL
                     RAPYLDMFHDMPHIPTPYTYRHPISGTDLANRLEETILAYGAENVAGFIAEPISGASL
                     GAAVPPDDYWPRIREICDRYGVLLIADEVLVGLGRTGSWWALDHWGIVPDILVTSKGT
                     AGGYFPLGFIAAKGEDVEQIRQKLGDFNHGGTFSHHAVGAAAGLATLNILQRETLIEH
                     SATMGAKLGALLREQIGAHPNVGDIRGRGLFWGIELVQDRATKQPFPLKDGLARRLWN
                     RAFDMGLVVYYSTGCADGKDGDVIMLGPPLIINETQTNEMVGILRDALYAELNV"
BASE COUNT         2222 a         3595 c         3900 g         2928 t
ORIGIN      
        1 gattgaaccg tttccccggc caaaggagag ccgataacga acgcccatcg acccaaccgc
       61 tcggccgcct cgtggtcgcg cggcgttgtc gtcggcgagg tcaggatgag cgtatccgac
      121 agcacaccgg ccagcagcaa gccggccagc tcaggcggcg cactcagacc ggcgtcgttg
      181 atgcgctcgg agatgagcgt cgaggtgctg ccgacgacgt caaccgtgaa gcgaatgggt
      241 atgcgcgtgg atggattgcc gaggcggtgg tgatccacga tctcaataat gtcggcctcg
      301 tcgatcgagc cgaccgcctg tcccggctcg ttgtggtcaa ccaggatgag ctgcatacgc
      361 ggcgggttga gcgcttcacg atggcgacaa atgcccacat agcggccggt ctcgtcgacg
      421 acccagaact ccgtccgttc ctcgcgcagg atgcgattga gcgcatcgcg aatacgggcg
      481 cttgccagga attgcggcac ggtgcggtcg acggcttcct ggcccggcat atccatcagt
      541 tcgccgatgc gcatttcctc gcgtcgcggg tgggagccga tcgtgcggct gaggaagtcg
      601 aacaggctga tgaccgtaac caggccgtat ggcttgccat cgctatcgac gattggcgca
      661 acgccgccgg tgcgattggc gatggaccag acttcgcgca atggatgctc cggcgcggcc
      721 gtattcaggc gatgggaaat cgttgaaaat cggggtgagg catcgattag aagcgtcggt
      781 tgctcaatct tgaggtggtt gagcacccat gttgtttgtg ggttcagatg gcctgcgcgc
      841 gctgcaatgg cgttctggcc ctgatcttgc agcagccagg cgtagcccat cgccgaggcg
      901 atcgcatcgg tatcggggtt cacgtggccg accacgtaga tggcgttgtt tttgtttcct
      961 gtcatgctcc gctatcctgc tctggaatgt ttgtgccctc aatgtaaagt gggcacacca
     1021 tctccataat atagatttaa ccttacgggt cggctaacaa tgcgatggtc gggtctcgtg
     1081 tggccgttca cgaccaataa tacccaatag ctgaacagtg cgcattacct ggcctcggac
     1141 gaatatcgtg ggtcaggttt ggcggggaat cggtctatat ccgaacggcc gacagtttgc
     1201 ccggccgcaa tttagccggc gttgacgtgt tgatttggaa gactgttcgg atactgacat
     1261 aatcccccac aaggtagttc agaatgatcg agattcatcc cctggagaaa ttcagcgcat
     1321 cgcgtcttcg gcagatcgcc tcaggttaca ccacgaatga gatttatcga gtcgaatacc
     1381 atgaagggca ggtagaaacc gtattcaggc taaccttgga gcctctgccc gaatcaaaga
     1441 gttttgtgtt tccattcgat gaagaagaat tgaagggcta tgaggcgctc atccccaacc
     1501 cgttttgcct gggggcgtat gacgacggct tgtgggtggg cgtggctttg gctgagccac
     1561 ggctatggaa caatacgctg tgggtatggg agtttcatgt ggccgaaacg tatcggggca
     1621 agggcatcgg ccgtcgattg atggagcgac ttgctgcgtc ggccgaggcg gccggattgc
     1681 gctcgctcat ctgcgagaca caaaacacga acgttcccgc catccgtttc tatcacgccg
     1741 tcggatatcg agtagaaggc gttgatattt cctactacac caatgaggac atgttgcccg
     1801 gccgaacggt ggccgtgttc atgaaacgca gattgacatg aacggcttga tacgctttgt
     1861 agaagagcgg cccggcttat cggccgctct cttgttcctg gggcttggcc ttgtgttttt
     1921 tgcggccgtt ctcctgcccc ccgccgggca agtgctggcc gggcacgata tgattggtta
     1981 ttactacgtg tattgggagg ccgtgcgcga ggcgctgctc tccggccggt tgcccttgtg
     2041 ggagccggat acttttggcg ggttcccctt tctggcccaa ccccagcaga acacgtttta
     2101 ccccattaac tggattaact tgatcctgcc cttgcgggtt ggcgtcagcc tctacatggt
     2161 atttcacgtc tggctggccg gcttcggcat gtatgtgttt ggccgtatca tgggcggccg
     2221 atggttgccg agcgtgctgg ccgggattgg gtttgccttc ggggggttgc tggccgggcg
     2281 gctatgggcg ggccaccaac ccgtctatgc ggtctttatc tggacgccgc tcatgctggc
     2341 tgcgctggtc tgggcggtta accggcagag ttggccgtcg gccgttgtgg ccggcgtgcc
     2401 gttcgctttg gcgatattgg ccgggcatat cccctctttc ctgtatgtgg gcatgatctg
     2461 ggccgctttt gccgccttcc tgttcatcac gcggcgcgat gctcgaacga cggtcattcg
     2521 cgcggccgtt attgccggga tcgtcgggct tgctctggcg gccgttcaac ttgcgccctt
     2581 tctccagttc agcgccacat ccggtcgggt tgcggaagcc gattatgaat tcgccacgga
     2641 ctattctctg ccgccggccc atctggtcac gctgctggtt ccgggattct tcggcgaacc
     2701 gctgcgggtc ggctattgga gcgtgccaac cttcgaggaa ctgacctatt acgcgggcct
     2761 ggtggctgtc ctgggcatcc ttctggcgct gcgacggccg accaaactct cctggttcta
     2821 cctgctgctc atcgtgttcg ggttatggct cgcgcttggc cgatatggta ttttgtatga
     2881 gcttgtctat cgctttcttc cgccgttccg cctggtgcgc gcgccggggc gggcggcctt
     2941 tctctatctc ttcgctgcct cggcactttt ggcccataca ctaaccgtct ggcgcgaaat
     3001 gcccgccgat agacggcgaa ggatgcttaa accgttttgg ccgacggcag tcatcgtgct
     3061 gggcctggcc ctgttcagcg cgctggcggc cacgggggcg atgttcatgg ccgtgcatcc
     3121 taccgatacc agcggccggc tctggcacca gattggtggc tactctctcg cgttggtcgt
     3181 tgtcgggaca ggcggtgcgt tgttgtgggc ctatctgacc ggggatcagg tggaaagtca
     3241 gcccccaggc tcatccaacg gagtatctcg gccccatggc gcgctctctc gccacacaat
     3301 gcgctttgct tttatcgccg ttgcccttgt ggcgcttgcc acaatcgata cctggtggtt
     3361 ttcctaccca atggtgcgaa ccgcgccaac tgtgccggag gcaatctgga cggacggccg
     3421 ccaactggtg ggtgatgtcg aagggcgcgt gttgccgtgg ggcgtgccgc tgtttagtca
     3481 gaacggcgca atgcgggtcg attggccaag cgtctttggc tatgactctt tggaacccgc
     3541 ggcccatatt gctctggctt cgtcggtgcc cgatccgcgt tcaacggcct atgatatcct
     3601 tggcgcgtcc catgttttgg ccggtgggcc gttggatgag tttactcagg gagagcaagg
     3661 gctgatcttg ttgggacagc agggggctgc ctgggtttat agccgaccgc gagcattgcc
     3721 gttggccagg ttggttaatc aggttgaaat cattgccgag gatgtggcca taattacccg
     3781 aatccatgaa cccggatttg accccgcaat cgtagccatc ctgaacgctc cgccaccctg
     3841 cacccttgaa ggtggcccac tcggtactgc caccgtaatg acccataggc caactcgttg
     3901 gcagatcgcg acgagcagcg cgggagaagc gctgcttgtg ctggcggaaa acgcctatcc
     3961 cggctgggag gtgcggatcg atggccagag ggcggagccg ttgacggcct acacgtccat
     4021 ccgggccgtt tgtgtgccgg ccggggagca tacggtggag tgggtttttc ggccgtggct
     4081 ctatggggca ggagcggtta tcacactggt ggcgctggcc ttggtcatct tcgctgccat
     4141 caggcttcga cgacctgagt cgtcagttcg ctgattattt caccggctgc gattagtgga
     4201 ggattttgtt tgcaaggcga tccgcctgcc tgaaggcccg ctccatctca tcaacatccg
     4261 gccccttcat gccctgggtc tctcgggtgc gaaagtaatc ggccatgacg tgtttcccgg
     4321 aagtgattaa ggcatcccgc cggtagtcac tcatatcgaa ctcggttgtt ccatatccct
     4381 ttgccggcag gcggcagacg aactgctcaa acgcctcgat aaccatcttg tcgtgggctt
     4441 gcgtcgccgt gtcgattaag ccgataatgc gttcgactgg cggcagatcg gtcaacgagc
     4501 cgttggcctc attggagggg tcagggtcta tgcctgggac gctcaactct tcgtcgatga
     4561 gaaggccaag cattccctgg ctggtctttg gccccatcag tgacacgaca tagggatcgc
     4621 tggagaggaa aagttcgatg gggaagttgg agagcaagcc gccgtccacg atgcggtggc
     4681 ccgccatcga gtagctcaga tagttgcccc attccggctg ccagatgact tcttgccaga
     4741 gcaagggcag gctcatcgac atgcgcatgc cccacacgac cggcagacgg ggcgcggtgc
     4801 gatggttcag caccagcagg cgatgggcgg tcgtatcggc cgccaccagg gacaactcgc
     4861 ggcccgtttt cagatagaac tcggcgaagg tatgattgct caaccggcgg acctcgccgt
     4921 cgggcgttgc ctgatcgagt ttggttcgcg cccattccac aaacgcatcg gccgaatacc
     4981 acccgcctct ttcgacaaat gaaaaaatgt gggcgagttc ttcctgggtc atgaggctga
     5041 ggatgaagcg atcgaatctg gattcaatga aatcgggcat ccagggtaga ttgattttct
     5101 ccagagctgc tcgcgtttcg ctgtgggcta tttcctcttt ggtgaagggc tttgggcggc
     5161 ccaggaagcg agtgaataca tgtttaccgg attcatcttt ctctttcagg gcgcgcatca
     5221 tctcgtcggc ggtgaaaccg gccgccagat aggtggcggc gatagctccg gctgatgtgc
     5281 ccaacagccg cccggccgtg tgccccagac gctcaaactc ctgtagcgcc ccgacgaata
     5341 ccatgccttt ggcgccgcca ccctcgaata ccagatcgta ctgcattgtc ctgacctacc
     5401 ggcgtgggtt aatgtcgctg attgtaccat agctggctat aggggcatcg ctttgcggat
     5461 gaccggcaat tagccttcct gggcatcgtt gcccaccttc ccagggtatg ctaaaacctt
     5521 gcgaggactc aatacgtata gcgcatcgag tcgtccacga ggctggaact atttttatga
     5581 gaaaacctga cactttttat gcacgattgg tagcagtatt gctgctgccg ttgttgttga
     5641 ttggcgctct acctgctgcc gcgcaggagg ctccggggga ttttatcccg tcggattgca
     5701 tgtttggcgg gatcgacctc ggtctgacga aaattggtgg tgagtcgctt ggctttgaat
     5761 gtggctacgt cgtggtgccg gagcgccatg ccaatccgga tgggccgacg atccgcatcc
     5821 ccgtcgctgt tcgccgggca actgcgccca acccgcatcc cgatcccctg gtgcttgccc
     5881 agggtgggcc gggcggagat gcgtttgaga tatttaccct gctcgtaccc agcacggata
     5941 tcgccgcgaa tcgcgatatc atcattttca accagcgcgg cacgccttat gccgaaccgg
     6001 agttgagctg cccggagaca gcggcggtct tgcctcaaac cctggctgcg accgaggaag
     6061 aggggaaccg gctttataac gaggccttga gtgcatgctt cgagcggctg caatcggagg
     6121 gtatcgatct ctcggcctat aacagcctgg aaaacgcggc cgatgtgccg ctggttgttc
     6181 aggcgctgga ttacgatgag tataacttct acggcgtttc ctatggcact ctgctgggcc
     6241 ttcacctgat gcgtaatcac ccggagggac ttcgctccgt catcctcgat agtgtaattg
     6301 cgccggatat taactttctc agcgaggttc cggcgagcga gaatcgggta ttggatgagg
     6361 tattcgctgc ctgcgaggcc gaccctgatt gccgcgagca gtaccctaat ttggaagagc
     6421 gtttttttaa ccttgtgcgt caatttgatg ataagccggt cacgcttcaa ttgcgggacc
     6481 ccgaaaccgg caacaaatat gagacctaca tggatggcgc cgcgctgcgc tccgtcatgt
     6541 tccagatgct ctacgtctcg cgaatgagcg ccgtgatgcc caaggtcgtg gctgatttgg
     6601 agaagggcga tacgcgctac atagagaaca tgtggccctt gttcgtcttc gatcagttga
     6661 tagcggaggg tatgtactat tcggtggttt gtgccgaaga tgccgatatc gacccggcgg
     6721 cgatttccgt cgattccttg cgtcctgaaa ttgcagccac ggcccgcgac gaaattcaat
     6781 cctatctcga cacctgcgcc cgctggcaag ttgagttatt gccgccgtcg gtggacgccc
     6841 cggtgattag cgatattccc acccttctgc tgtcgggtcg ttttgatccg gtgacgcccc
     6901 cggcatttgc cgcggccgtc gctgccgggt tgccgaacgg aacagcgttg gtcgatccga
     6961 cggcgagcca tggagtcgca tttatgaccc agtgcgccaa tgaagtagtt ggggcgtttc
     7021 ttgatgatcc catgaacccg cctgatgggg cttgctttga tgcccagaaa ccgctcaagg
     7081 cagttccgcc aaacgcagtc accgtaccgc tgctggccgg ggttaacagc ctggattccg
     7141 gaaccatttc agcgtttgtg ctggccggca tcttgctggt gatcgtcctg tcgccggtgc
     7201 tcctgtggcc tctcgtgttc gccgcgcgtg cttttggcgg tgatccccca ccacaacgtg
     7261 ccccgaaaga ccggcgagtg cggtggatta gtcgaattgt tgtcttgctg tttggggtgt
     7321 tgggcctgat tttcgctgtc ggcctgattg gctccattgt gatggttgta gccaccgatc
     7381 agacgttgat gacggcgctg tcattgcccg cttcggccgc gcctatattg tgggtgcctg
     7441 tatttatgct gctaatggct gttttgatga tcgcggccgg tgtcatgctt tggcgtcacc
     7501 ctggcgcggg cactaccctg ggaaaagttt actattccat catcgttatc gccgccgtag
     7561 cgcttttggg cgttatcgga gcgcaggggt tgctgttgcc gccactatag gggaacagtc
     7621 cgctctcgtt tgtcgtcctg actgatagat cgtccaaatc tatcacaggc aggtgcgaca
     7681 tgattccaaa agagagccgg ttcaagcgga tgctacgcgc ggtcattttc tgctcagcag
     7741 tggccgcgct ggcgatcttt ctcatagatg atttcaagcg gggcacgaca gaaccgccgg
     7801 cagcccggtt attgcccgat ttgcccgctt atcggcaggt agaggggcaa accatcaccg
     7861 gctatatcgg cgcactggcg gaaggcgcgg cgcttctggc cggccatccg caactggccg
     7921 tcacggtcgg cacgcttgat caggcgatcg actgctatca ggagatcggc ggagtgcgag
     7981 cgcgggtata cagccatgcc gaacgccctc tggaggcggg tctggtggct gtggccgatg
     8041 aggccaggct gaacgacccc gatagcctat tccgttgcgt cgctccggct acgctgaatc
     8101 ttgagggaac ccaggaaccc gtcatcgaac catgcacggc tgcttatacg atggtgaacg
     8161 aagaggggac gttctacatc atctatgccg cgtccaccta tggcgtctgc cgtgattttt
     8221 gtgaatcgct ggaaggttgc gatgtccacg ctataaattc attagaattt tcattgcgag
     8281 acctgcgggg atggctataa tgaacaggtt tgcccgaacg caaaaggtgt ccggacatcg
     8341 aggttttaat gagaagtcag agagatattg tttggctggc gttcatcatc attctggtgg
     8401 cgggcgctat ttgggtgatc acaaaccccc ggttccccat ccgctatggc cttgatctgc
     8461 aaggtggctt gcaggtgttg atggaggcgg atgtccccgc ggaccaggtg gtgacgtcgg
     8521 atgcgatgga cacggcccgc cagatcgtgg atcggcgcgt gaacgcgatt ggcgttaccg
     8581 agccgttggt gcaggtcgag ggcaatcgtc gtattctggt cgagctgccc ggcattgagg
     8641 acccggaaca agccctcgcc ctcattcagg agactgctct ccttgagttt gtggataccg
     8701 gcgagttctc cttgcccgaa gggatgtgta ttcgcacttc gttgaatgaa ggcccgtctc
     8761 gctgcgagtc ggaaacggtc gatggtgaag ctgttgccac gactgaagag ccgccgacgt
     8821 ttccgacgat cctgaccggc gccggcctgc gcgatgcagg cgtaacgtcc gatcaactgg
     8881 gacagtactt cgtggatttc actctgaccg aagagggtgg gaaggtattc gcggagcata
     8941 cgcgcgccaa ccagggcaag tacctcacca tcgttctcga caaacgtgtc gtctccagcc
     9001 cgacgatcag cgcggtgatt gaggatcgcg gcacgataac gggcaacttc accctggaag
     9061 aagcccagca cctggccacc cagctacgct ttggtagttt gccgatcccg ttgcgcatcg
     9121 agagtacccg gcagatcggg gccacgttgg gcgaacaatc catcgacgcc agtatccagg
     9181 ccgggacaat cggcgtggcg atggtcttgc tgttcatgct tatctactat cgcctgccgg
     9241 gcgtgttggc tgacgtcgct ttgatcgtct acgtcctgct cagctttgcg atatttatgg
     9301 gtgtgggagt cacgttgact ctgccggcca tcaccggctt cctgctctcc acaggtatgg
     9361 ccgtagacgc gaacgtgctt gtgttcgagc gtatcaaaga ggagatgcgc cggggcgcgc
     9421 acctcgaaga tgctatcgat accggcttct cacgtgcctg gaattcgatt cgcgattcaa
     9481 atgtcgctac cctggtgatt tgcctgatcc tgtggacgtt cggtcgcaat ttcggcgcaa
     9541 gcaccgttga ggggtttgcc gtcaccctgg ctattggtgt cttcatcagc atgttcacgg
     9601 ctgtgctggt cactcgcaca ctggtgcgcg tgttcctggg ccggaacgcc aaccgccttc
     9661 agggccagaa atggctgatg ggcgtctaat cgtcggcgag gtaagaaagt catgtctttt
     9721 gtttatcata tcgtagagaa tcgtcgccga tatttcctgt tctccggcac cttgctggcc
     9781 ctggggctga tcgccatgat cgcctcggca gtcatgaccg gctccccatt tcggattggt
     9841 gtggacttcc gaagcggcac tcgattcgag gtgcaatttg aggaacccgt aacagagaac
     9901 caaattcgcg atgtcttcgc cgaatacgga atcaccagcc cctcgatcat cgccctgcgc
     9961 ggcgaaaacc tggaaaatgc ctggcagatc cgcggcgagt ttgttgctcc cgagacggcg
    10021 caggccatcc tcaatagcct tggtgaaatg gctcccttgc gcaaggaagc gtcctcggtg
    10081 accagcgtca gcgcggccgt cggcaatgag gtgactcggg cggctatcgt cgcggtggcc
    10141 ttctccgccg tcgtcattct gttctacatt gtcgtcgtgt ttcggcaggt gcccaacacc
    10201 ttccgatatg gcacgacggc cgttatcgcc ctccttcatg atctctttat tgtcctgggg
    10261 tttgcggcaa tcaccggatt gttgctgggt tgggagatcg atgcgctgtt cctgacggcc
    10321 gtgttgacca tcgccggctt ctcgttgcag gatacgatcg tgctgttcga ccgtatgcgc
    10381 gaaaatatct ctcggcgtcc gttcgagaag atcgatctga tcgccaatcg ttcgattgtc
    10441 gagactatcc accgttctct cgtgacgcag ctcaacgcga tgtttgtcat ggtcgccatc
    10501 ctgttgtttg gcggtctgtc ggtcagacct ttcattgcca cgctgttttt tggcttattg
    10561 ttgggaacct atagctcgat cttcatcgcc gtgccgctcc tggtgacttg ggaagaacgg
    10621 atcagcaccc gcggccgcgc cgcataaaga cgccatgcaa gcgcttgtgt tacgcgacgg
    10681 tcaactggct taccatgccg attggcccca gccgattccc ggccccgaag aggcgctcat
    10741 tcgcgtctct ttggccggca tttgttcaac ggacctggaa cttgtgaaag ggtatgccgg
    10801 tggtttcaac ggtatcctgg gccatgagtt cgtgggcgta gtcgagcagg tgggggcaca
    10861 ggccccgacg gagtggatcg ggcagagagt ggtcgggtca atcaatatcg gctgtggcca
    10921 gtgcctggtt tgccagagcg acggccccga gcactgtccc gaccgcacga cgctgggcat
    10981 ccaccatcgc gacggcgttt ttgccgatcg ggttgtcttg ccggtagcga acctgttttc
    11041 cgtgcccgat gttgtatccg acgaggaagc tgtcttcacc gagccgcttg ccgcggccgt
    11101 gcgcgtgcgc gagcaagtcc acatacggcc cacccaacgc gcggccgtcg tggggccggg
    11161 gcgtctgggg ctgatggtgg ctcaggtgtt ggcgttggcc ggcggggatg tcatcgtgct
    11221 gggtcgccgc ccggaatcgc tggaattgcc aaagcgcctt ggattatcgg ttggcctggt
    11281 cgatgagtta tccgacaata gcatggatct ggtcattgaa gctacgggta acgacgccgg
    11341 gttcgcccat tccttgcgcc tggtgcggcc gctgggcacg ctggttctca agagcacatt
    11401 ccatggcaac gccaatatca acctgaccaa gctcgtggtt ggtgagatca acgtggttgg
    11461 ctcgcgctgt ggcccgtttg ggccggcgct gcgcctgttg gaggcaaacg cacgagcggc
    11521 cggcagcctg atggccggag ccggcgttca ggtgcggccg ctcgtggacg cggaatatgc
    11581 cttgcgggac ggcctggccg cgttcgagta tgccgctcga ccgggcgtac acaaggttct
    11641 gctgcgaccg aattagacgt ttaattcggc gtaaagcgcg tcgcgcaaaa tccctaccat
    11701 ttcattagtc tgtgtttcat tgatgatcag cggcggcccc agcatgatca cgtctccgtc
    11761 tttgccgtcg gcgcagcccg tcgagtaata gaccaccagc cccatatcga acgcgcggtt
    11821 ccatagccgt cgggccagcc cgtccttgag cggaaagggc tgctttgtcg ctcgatcctg
    11881 caccagttcg atcccccaga acaggccgcg gccgcgaata tcgcccacat tgggatgtgc
    11941 gccgatctgc tcacgcaaca gggcgcccaa tttcgcgccc attgtggctg aatgctcaat
    12001 cagggtttcg cgctggagga tgttgagcgt agccaggcct gccgccgcgc ccacggcgtg
    12061 gtggctgaag gtgccgccgt ggttgaaatc gcccagcttt tgccggattt gttccacgtc
    12121 ttcgcccttg gcggcgatga aacccagcgg gaagtagccc ccggccgtgc ctttcgatgt
    12181 gaccaggata tcgggcacga tgccccagtg atcgagcgcc caccaactgc cggtgcgccc
    12241 cagcccaacc agcacttcgt cggcgatgag gagcacgccg tagcggtcgc agatctcgcg
    12301 gatgcgcggc caataatcgt cgggcggtac ggccgcgccc agcgacgcgc cgctgatcgg
    12361 ctcggcgatg aacccggcaa cattttcggc accgtaggcc aggatagttt cttccagtcg
    12421 attggccaga tcggttccac taatcgggtg gcgataggtg tagggcgtgg ggatatgggg
    12481 catatcgtgg aacatgtcca gataaggcgc gcgcagcccg gctcggccgc tgacggccag
    12541 cgcgcccagc gtcatgccgt ggtagctttg ccagcggctg aggacgatgt tgcgtttctc
    12601 ctcgccgcgc gccatctgaa tctggcgagc cagtttgatg gcgcc
//
LOCUS       DAOMQG010000062        12629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2149_length_12629_cov_11.771751, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000062 DAOMQG010000000
VERSION     DAOMQG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_12629_cov_11.771751"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PLC06_05395"
     CDS             complement(<1..1272)
                     /locus_tag="PLC06_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89913.1"
                     /translation="MSVVILGAFALVLAHARANPGDFDPTWGEEGVVLTDFDGLDDTA
                     NTQVLMPNGKLVAAGWVNTYPGDFGLVRYLRDGSLDPSFGDGGRVVTAFSDNPELVDA
                     AWAINARPNGGVHLFGETCDADYVVCQFAMAAYLADGSLDESFGEGGLVTTSIEDVAA
                     VLAWPTRNILQSDGKVVAGGIALYENNQVDIVLLRYNPDGSLDENFGDGGISIIDFEG
                     KSHFPQDIAALPGDRIMIVGGVTDEFEGNTYNSDTAFMARINNDGSTDLSFGGGDGYI
                     TWKYGDEPAHFERTLVASESEMFIAGSAAEDCAFLRFDLDGALDTTLGDDGWVLIDSG
                     LVDGCTDATLTVDGQIALTGTSRPEESDSGVATSSGRYHNRMSIRGAGPAGSSPAARQ
                     DEFYDHLVALYNADGTPDATFGDDGLVRFAVD"
     gene            1669..4320
                     /locus_tag="PLC06_05400"
     CDS             1669..4320
                     /locus_tag="PLC06_05400"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HRO89914.1"
                     /translation="MVEESFGDILRTLRRNSRDALYGGQLSQNRLADLLSEETGMVYS
                     RAAISDWERGKGHIHKDARPVLVGLVKILHQAGGLATRREAERWLAAGNYRALSAAEA
                     AGIDPEWSAPPAGEPAAGRPFLPPLMPQHPIIGRVEALGKLKEWLFDNRSLALSSING
                     LPGVGKTALALLAAHDPDVRQRFPDGVLWVGLGTNPDIFALLGRWAKAVGIPEEEVAK
                     LGAQRMRVDAIHQTIRNRQMLIVIDDVWETRHALPFKLGGAQSAHILTSRQPPIAAAF
                     AGPHAMTVRELSEAQGLELLRWLAPRVITRNEADARHLVALSGGLPLALVLIGNALRL
                     NDLSGSERRIRGTLDMLKWADERQRIEQTDSLVAGELHPDLEPGTAISLYAIIQVTDN
                     RLGSEARAALRGLALFPPKPNSFSEAAALSVTGATTETLDLLVDHGLLETVERDRYAL
                     HQSIHDYAGLEPHDPAREESLITYYQSCIDLNRENRQVLERELDNIQAALQLAAAEGR
                     QEALWGLLESLMPFFSDRGYYDLAMTMLERVEVGVTGINPAVKHYLGRLELLRENADK
                     AQAHWEAGLIAARLADDRVMVGRLLNSMSQLASQRRDFATAAAYLDESAALAQAAGDW
                     EEVARMLGNFGRLAYVRDDFDIAESYLRRAQEVAATHDLHSLSSGILNLLGIVLMSKG
                     DLEAAEQTLQAGLEIARAHRLEARISTLLANMGELLNEMGRFDAATAYLEEGVELSRR
                     LNDPGQEGHMLLDLGIAKAAQGAYEAAIPHFSAAHELAAGLDNPWQVALVKARWGIAA
                     LDAGDAEQARPLLVRAAELAPNVASSAKIEGLSRFGLAKLAIADGDRETAAAQSAAAL
                     AVLQTHGLAPAVEVAAWRAGQGFSSPP"
     gene            complement(4330..4866)
                     /locus_tag="PLC06_05405"
     CDS             complement(4330..4866)
                     /locus_tag="PLC06_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89915.1"
                     /translation="MSGELLATVIAGLMIDSSVTQQQAADTGRIVGALVRRGVAVDVY
                     PTRAELLASADAAGFSVGFIPPSGAQARLSGAAIDDETLRDDLQALFTGEGVFLVTGA
                     LQAGGFTIPTVPCFNGEPSTIVSFTSAEDFEQKVLAGEMSWLSAEQLAAVREGYLFNC
                     GLEAACPGTLGPKCPYIG"
     gene            complement(5054..5785)
                     /locus_tag="PLC06_05410"
     CDS             complement(5054..5785)
                     /locus_tag="PLC06_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRO89916.1"
                     /translation="MTTELVVFLMGALFNLMIAVIITRFIYYPLTQTKSFVFTFIAFN
                     SIVYFVLGLLTSTALGVGVGFGLFAIFSVLRYRTDEMPIREMTYMFILVALPVVNSVL
                     ITTNDYDTLLVVNAFIIAMLYVLEKGWGFRFEGNKRIVYDMIEYITPANEGRLLADLR
                     RRTGLMVKRVEIERIDFLRDVADIKIYYDEPHRKDHGAGELIAYEEPVVRYADMPMNA
                     ATTTNGVSAPARAGMNHTHSSVSAD"
     gene            complement(5787..6650)
                     /locus_tag="PLC06_05415"
     CDS             complement(5787..6650)
                     /locus_tag="PLC06_05415"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRO89917.1"
                     /translation="MLIEQLYDSTYTVPRGKLRRLEASTTYRRSVEDFSGLWQFMRLA
                     SSMEPIGLDGLQEVALMNRRETKYVLREQDLLNALVPLTARYWVLEIDGIRLHRYLSL
                     YFDTPSFFLYHRHRAGGKHRYKVRAREYTDTRLAFLEIKHKISELRTEKSRVQTPQFL
                     TDLSSEFDEFVQDHFPLPVEELEPKLLNAYRRITLVGKYNPERVTLDLGPRFYADSGN
                     ATLPGIAIAEVKYENNGRGSDFMRRMREMGLRPMGFSKYCVGVSMLYPHVKNNYFRPT
                     MRAVGKIMQGD"
     gene            complement(6835..8532)
                     /locus_tag="PLC06_05420"
     CDS             complement(6835..8532)
                     /locus_tag="PLC06_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRO89918.1"
                     /translation="MARIEADGRFGTGVLIEDRYILTNAHVVWPATEVNVTFPDGEKH
                     VAPVAGVDLLVDLALLGPIDTSIVPLSLTDGEQLAVGSPLLLIGYPGDVTRQPRPALT
                     QTLVSRLREQESFGMTYLQVDSPVAGGQSGGIAVTMEGDIIGLTGHRVTEVGFGIVTS
                     AADILPRMETMLGEPTGAFRGAKPERGNVQHKFEIADNLAHRAFVVDVPIGTLITAEV
                     SGDGDAVLIIQDTQDIDSVISDEPYGGVEKARLTTTTGGPHFVIVRQHNKWPTTYTLK
                     SNQTLIPYVDPDDGHALKVGEVYQGMSDYPVDVDVYSLDMTKEQAVNINLSSMLIDPY
                     LLVWPVALGPEGLVEDSDSGDGLFNWDAEMTYLAPNTGGHRILATDMFGRRAGGYQIT
                     IREPYADAPTPISPLPTMTPAPSPIGPLMRYESSDFPFAISYPASYDSDSDVEFCRYF
                     SDCFMARNGQAIMGILESELTDNDGRPVTLEDLRDHLETNQIEMGLKLIGHDDLITPD
                     DTSALLSTFEDPKTGVRTRIFTYLLEPNLMFRVVFMYQNASYEEIADYLIRTFEVIR"
     gene            complement(8836..9432)
                     /locus_tag="PLC06_05425"
     CDS             complement(8836..9432)
                     /locus_tag="PLC06_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89919.1"
                     /translation="MTTPAPPPDTELSRSIIAAEFDYIAQAAFQANEDRARAWQYFFV
                     TFATLIAALLSTQVETAAQQQLYITFAIIFVLLSALGLITILQLARLRQAWLESVRAM
                     NQIKERLIADDPSLADYFRWRNATIPPAFKWRSFGFLQALSVALLSGLAIGAAVSFGA
                     LASGAVVVPWGWSLLAAVGASAIILLLGYIRPLEKAVS"
     assembly_gap    9576..9675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9708..10154)
                     /locus_tag="PLC06_05430"
     CDS             complement(9708..10154)
                     /locus_tag="PLC06_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HRO89920.1"
                     /translation="MPTYNKNGNEVTIPSTHLDLITGPVYVVFTTVARDGQPENTVVW
                     CSWDGEHVLVNTVEGRRKYENVRHNPLVAVTAIDPTNPYRWIDVRGCVEAIVPDEGNA
                     NIDYHARLYDGVEHYYGDVAPLERKDKETRVILMIKPTSVVVYPHG"
     gene            10450..11007
                     /gene="infC"
                     /locus_tag="PLC06_05435"
     CDS             10450..11007
                     /gene="infC"
                     /locus_tag="PLC06_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRO89921.1"
                     /translation="MNRQIRAREVRLIDHNGVNRGVLPFFDALRIAEDAELDLVEVAP
                     NANPPVCRVLDFGKFAYEQTKKEREARKHQKKIEIKTIRLSPRTGDYHKDIGVRNARK
                     WLEEGKKVKFMVRFKSREISYPEIGQKALQEIADDLSDIAIVEQQPTLEGWRMTLTLS
                     PESAAPPKGQAGSDAPKEEPEATQG"
     gene            11103..11348
                     /gene="rpmI"
                     /locus_tag="PLC06_05440"
     CDS             11103..11348
                     /gene="rpmI"
                     /locus_tag="PLC06_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRO89922.1"
                     /translation="MPKGTKKYKLKTYKAASKRFRVTGTGKIVRTKGGKSHLRRRKSK
                     RTKQQFSEMIVVTAPGDIRRIKRLAPYLKKYKANPPG"
     gene            11439..11792
                     /gene="rplT"
                     /locus_tag="PLC06_05445"
     CDS             11439..11792
                     /gene="rplT"
                     /locus_tag="PLC06_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013507016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRO89923.1"
                     /translation="MRVKGGFVTRRRHKKILDMTKGQWGSRSKLFRRANEAMMKSYWY
                     AYRDRRNKKRDFRRLWITRINAATRIHGLSYSRFMHGLKVAEVRLDRKALADLAVRDE
                     AAFAAVVGVAKNALA"
     gene            11934..>12629
                     /locus_tag="PLC06_05450"
     CDS             11934..>12629
                     /locus_tag="PLC06_05450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89924.1"
                     /translation="MTLDPTAREFDPAIRKLRQTGWILLILLAAAALRLVALPSAPPG
                     MTGIPPGMTHDEADHGLTAWEIVNGARALYFTVGYGREPLYDYTTALLMAGTGPTILA
                     ARMTSVYFSLLMIAAVYAWARRAFGAPVALLTAAGLAAGFWPVMAGRQALRSIALPAL
                     FSLAVLVFWWGLLEGEKGRKGEREKGRKIAPQHPFPLSPLLFLAAGILLGLSIYTYIP
                     ARVMWPAFPALALY"
BASE COUNT         2691 a         3768 c         3645 g         2425 t
ORIGIN      
        1 atcgaccgca aaccgtacca acccgtcgtc gccgaaggtc gcgtccggtg taccatctgc
       61 attgtagaga gcaacgaggt gatcgtaaaa ttcatcttga cgagcagccg gagaacttcc
      121 ggccggcccc gcgcctcgta tggacatgcg attatggtat cggccagagg aggtagccac
      181 ccctgaatcg ctttcctcag gccgggaagt cccggtgagc gcgatctggc cgtcgacggt
      241 caaggtggca tcagtacaac catccaccag gccgctgtcg atgagaaccc atccgtcatc
      301 ccccaatgtc gtatccagcg caccgtcaag atcgaaacgc aggaatgcgc aatcttcagc
      361 cgcgcttccg gcgatgaaca tttcgctttc cgaagcgacc agcgtgcgct caaaatgggc
      421 cggctcatca ccgtatttcc aggtgatgta accgtcgccg ccgccaaagc tcaaatccgt
      481 tgagccatca ttgttgatcc gggccataaa cgctgtatcc gagttatatg tattcccctc
      541 gaattcatct gtgacgccgc cgacgatcat gatcctgtcc ccgggcaggg ccgcgatgtc
      601 ctgaggaaaa tgcgatttcc cctcaaaatc aatgatggaa atacctccat cgccgaaatt
      661 ttcgtcgagc gagccgtcgg ggttgtaacg gaggagcacg atatccacct gattgttttc
      721 ataaagggca atcccgccgg ccaccacctt gccgtccgat tgcaggatgt tccgcgtcgg
      781 ccaggcaagg acggctgcca catcctcgat tgatgtggtc accagcccac cttcgccgaa
      841 cgattcatcc agcgaaccgt cggccagata ggcagccatg gcaaactggc acaccacata
      901 gtcggcatcg caggtttcgc cgaagagatg caccccgcca tttggccggg cattaatcgc
      961 ccaagcagcg tcgacaagtt cagggttgtc gctaaaagcg gtcacgacgc gccccccgtc
     1021 gccgaaggaa ggatcgaggc tgccgtcccg cagatagcga acaaggccaa aatcgcccgg
     1081 ataggtattc acccaaccgg ccgcaaccag tttcccgttg ggcatgagaa cctgggtatt
     1141 ggctgtatca tccagcccat caaaatcggt gaggacgacg ccctcttcgc cccacgtcgg
     1201 gtcgaagtcg ccggggttgg cccgcgcatg ggccagaacc agagcaaatg cgcccaaaat
     1261 cacaactgac attaacgcga ctgtcttttt catttttaat ctccttgaca tacttaccga
     1321 tctctattga gttacgccga tggaatggag ctgtagattt cgatgcagcc ctttccctat
     1381 ccatgtttag agagggatta cgaatcctct atttcatatg taaccccttt gtaatggttg
     1441 tccggcgcga cgcgacaatc acccgaggtg agtgattgtc gcgcgcccca gcgactggct
     1501 accctcaaac tggcgcatgg tgtgcggtgt gttttgaatg taagcaggcg gttcatgtca
     1561 acgaaaattt ctgctatcat gttagaattg ggcgaccacg cggaagagag cgaatcggcc
     1621 gcatcgccgc ggaaactttc cgcatcaaca ccacacggag gacgccaagt ggttgaagag
     1681 agttttggcg acatcttgcg aaccctgcgg cgaaattcgc gcgacgcgct ctatggcggg
     1741 caactctcgc aaaatcgcct ggccgatctg ctgtccgagg aaaccggcat ggtctattca
     1801 cgggcagcca tcagcgattg ggaacggggc aaggggcaca ttcacaaaga cgcccggccg
     1861 gtgctcgtcg gcctggtcaa aattctgcat caggcgggtg gtctggccac gcgccgggaa
     1921 gccgagcgat ggcttgccgc cggcaactac cgggcactga gcgccgccga agcagccgga
     1981 attgatccgg aatggtcggc gccacccgcc ggggaaccgg ccgccggccg cccatttctg
     2041 ccgccgctga tgccgcaaca ccccatcatc ggccgggtag aggccttggg caagctcaag
     2101 gaatggctgt tcgacaatcg ttcgctggcg ctttcatcca tcaacgggct gccgggtgtc
     2161 ggcaagacgg cattggcgct gctggccgcc catgatcccg atgtccggca acgattcccc
     2221 gatggtgttc tctgggtagg gctgggcacc aatcccgaca tcttcgccct gttgggtcgc
     2281 tgggccaaag cggtgggcat tcccgaagaa gaggtggcga agttgggcgc gcaacgtatg
     2341 cgcgtcgatg ccatccacca gaccattcgc aatcgacaga tgttgatcgt catcgacgac
     2401 gtttgggaaa cccgccacgc gctgccgttt aagctgggcg gcgcgcaaag cgcccacatc
     2461 ctcaccagtc gccaaccgcc aatcgccgcc gcgttcgccg gcccgcatgc catgaccgtg
     2521 cgcgaactgt ccgaggcgca aggcctggag ttgctgcgct ggctggcccc gcgcgtgata
     2581 acccgcaatg aggccgacgc ccgccatctg gtcgcgctca gcggcggcct gccgctggca
     2641 ctggtgctca tcggcaatgc gctgcgcctc aacgacctaa gcggctcgga gcggcgcatc
     2701 cggggcacgc ttgacatgct caaatgggcg gatgagcgcc agcgcatcga gcaaactgat
     2761 tcgctggtgg ccggcgagct tcatccggac ctggaacccg gcacggcgat ctcgctttac
     2821 gccatcatcc aggtcaccga caaccgtttg ggaagtgagg cgcgggccgc attacgcggg
     2881 ctggccctgt tcccgcccaa accgaacagc ttcagcgagg cagccgctct cagcgtgacc
     2941 ggcgcgacga cagagacgct cgatctgctg gtcgaccacg gtctgctgga gaccgttgag
     3001 cgcgatcgct acgccctgca ccaatcgatc cacgactacg ccggtctgga gccccacgat
     3061 ccggcgcggg aggaatcgct gatcacctac taccaatcct gtatcgatct caatcgagag
     3121 aatcgccagg tgctggagcg agagctggac aacattcagg ccgccctgca actcgccgcc
     3181 gcggaaggcc ggcaagaagc gctgtgggga ttgctggagt cgctgatgcc cttcttcagc
     3241 gaccggggct attacgatct ggcaatgacg atgctggaac gggtcgaggt cggggtcacc
     3301 ggtatcaatc cggcggtcaa gcactatctg ggccggctgg aattgctccg cgagaacgca
     3361 gacaaggcgc aggcgcattg ggaggccggc ctgatcgcgg cacgcctggc agatgaccgg
     3421 gtaatggtgg ggcgattgct caacagtatg agccagttgg cgagccagcg gcgggatttt
     3481 gccacggccg cggcctatct ggacgagtcg gccgctttgg cgcaggccgc cggcgattgg
     3541 gaagaagtgg cgcggatgct gggcaatttc ggccgcctgg cctatgtgcg tgacgatttc
     3601 gatatcgccg aaagctacct gcggcgcgcg caggaggtgg cggcgaccca tgacctgcat
     3661 agcctttcca gcggcatcct caatctgctg ggcatcgtgc tgatgtcgaa aggagacctg
     3721 gaggcggccg agcaaaccct tcaggcgggt ctggagatcg cccgcgcgca ccggctggag
     3781 gcgcgcatct cgaccttgct ggccaacatg ggcgaactgc tcaatgagat gggccggttt
     3841 gatgcggcca ccgcctatct ggaggagggc gtggaactgt ctcgccggct caacgacccg
     3901 ggccaggaag gccacatgct actcgacctg ggcattgcca aagccgctca gggagcgtat
     3961 gaggcggcaa tccctcattt ttcggccgct catgagctgg ccgccggatt ggacaacccg
     4021 tggcaggtcg cccttgtcaa ggcgcgttgg ggtattgccg cgctggatgc aggcgacgca
     4081 gagcaagccc ggccgctgct ggtacgggcg gccgaactgg cccccaacgt cgcctccagc
     4141 gcaaaaatcg aagggctaag ccgcttcggt ctggcgaagc tggccatagc ggacggcgac
     4201 cgcgagacgg ccgccgccca aagcgcggcc gcgctggccg tgctgcagac ccacggcctc
     4261 gcgccggcgg tcgaggtggc cgcgtggcgt gccgggcaag gattcagttc gccgccctaa
     4321 gcatcggcat tagccaatat agggacattt ggggccaagc gtccccgggc aagccgcttc
     4381 caggccgcag ttgaacaggt agccctcccg gacggccgcc agttgctcag ccgatagcca
     4441 actcatttcg ccggccagta ctttctgctc gaaatcttcg gccgacgtga agctgacgat
     4501 ggtcgaaggt tcgccgttga agcagggcac ggttgggata gtgaacccac cggcctggag
     4561 cgcgccggtg accaggaaaa caccctctcc ggtaaacaac gcctgcagat catcgcgcaa
     4621 cgtctcatca tcgattgccg cgccactgag cctggcttgc gcgccgctgg gcgggataaa
     4681 tcccacgcta aatcccgccg catcggcgga tgccaaaagc tccgcgcgcg tggggtaaac
     4741 atccaccgcc acgccacgcc tcacaagcgc cccaacaatc ctgcccgtgt ctgccgcctg
     4801 ctgctgcgtc acagacgagt cgatcatcaa accggcaatg acagtcgcca ataattcacc
     4861 actcatggaa tttttctcct gctcccgcat agccgaactg gacaataatg ccgggtgagt
     4921 agacaccaaa ggcacacacc cggcaagcta tatccaaacg aacccctcca gcggcaaata
     4981 gttgcaaaaa tgggcgatca gaggtgcaac acctctgatc gcccatctgt caggcctgtc
     5041 cgtcaccggc gtctcaatcg gccgatacag acgaatgagt atgattcatc cccgcccgcg
     5101 ccggggccga gacgccgttc gtcgtcgtgg ccgcgttcat cggcatgtcg gcatagcgca
     5161 caaccggctc ttcataagcg atcagctcgc cggcgccatg atccttccgg tgcggttcgt
     5221 cgtagtaaat cttgatatcg gccacatcgc gcaggaagtc gatgcgctcg atctccaccc
     5281 gcttcaccat cagcccggtg cgccgccgca ggtcggccag cagccggccc tcattggccg
     5341 gggtgatgta ctcgatcatg tcatagacga tccgcttgtt gccctcgaag cgaaagcccc
     5401 agcccttctc cagcacgtaa agcatggcga tgatgaacgc attgacgacc agcagcgtgt
     5461 cgtaatcgtt ggtcgtgatg aggactgaat tgacgaccgg cagcgcgacc agaatgaaca
     5521 tgtaggtcat ctcgcgaatg ggcatctcgt ccgtgcggta gcgcagcacc gagaagatgg
     5581 cgaacagacc gaacccgacc cccacaccca acgccgtgct ggtcagcaac cccaggacga
     5641 aatacacgat agagttgaag gcgataaacg tgaacacaaa gctcttcgtt tgcgtcagcg
     5701 ggtaatagat aaatcgcgtg atgatcacag cgatcatcag gttgaacaga gcacccatca
     5761 gaaagaccac gagttcggtt gtcatttcaa tctccttgca tgattttgcc gaccgcgcgc
     5821 attgtcggtc tgaaatagtt gttcttaacg tgcgggtaga gcatcgacac gcccacacaa
     5881 tacttgctga atcccatcgg ccgcaggccc atttcgcgca tccgccgcat gaagtcggag
     5941 cctcggccgt tgttctcata tttcacttcg gcgatggcga tgcccggcaa ggtcgcgttg
     6001 ccgctgtcgg catagaaccg cgggcctaaa tcgagcgtca cgcgctcagg gttatacttg
     6061 ccgaccagcg tgatccggcg ataggcgttc aacaatttag gctccagttc ttccaccggc
     6121 aaggggaagt gatcctggac aaactcatcg aactccgacg agaggtcggt cagaaattgg
     6181 ggcgtctgca cccggctttt ctccgtccgc aactcgctga tcttgtgctt gatctccaga
     6241 aaggccagcc gggtatcggt atattcgcgg gcgcgcacct tgtagcgatg cttgccgccg
     6301 gcgcggtggc gatggtagag gaagaacgac ggcgtatcga aatagagcga caggtagcga
     6361 tgcaagcgaa tgccgtcaat ttccaatacc caatagcgcg cggtgagggg cacgagggcg
     6421 ttgagcaggt cttgctcccg caacacgtac ttcgtctccc ggcgattcat cagggccact
     6481 tcctgcaagc cgtccagacc aatcggctcc atcgaagaag cgagcctcat gaattgccac
     6541 aagccgctga aatcctcgac cgaccgtcga taggtcgtcg acgcctccaa ccggcgaagc
     6601 ttcccgcgcg gaaccgtata agttgaatcg taaagttgct cgattaacat cacatctccc
     6661 agccgtgacc ttgttcactg ctgtctatga ggtgacgttt acggggaata agtaacgatc
     6721 ggggggaaac caatagaaaa cgggcttatg tcgtcgggat ctcaggcgac aaaagcccgc
     6781 atcattgggt ttcaataatt acattccacc ctcgatgggg aagccgaaat cgacctatct
     6841 gatgacctca aaagtgcgta tcagataatc agcgatttct tcatagctcg cattctgata
     6901 catgaagacg actctaaaca tgaggtttgg ttccaggaga taggtgaaaa tcctggtacg
     6961 cacgcccgtt tttgggtcct caaaagtgga gaggagggcc gaggtatcat ccggagtgat
     7021 cagatcgtcg tgaccaatca acttcagccc catctcaatt tgattcgttt ccagatggtc
     7081 gcgcaaatct tcgagagtca caggacggcc gtcgttgtcc gtcaattcgg attccaggat
     7141 gcccataata gcttgcccgt tccgcgccat aaagcaatct gagaagtacc ggcaaaactc
     7201 gacatcggaa tcggaatcgt aactggccgg atagctgatg gcgaatggaa aatccgaact
     7261 ttcatagcgc attagcggac cgatgggact gggggcgggc gtcatggtag gcagcggaga
     7321 aatcggagtc ggcgcatcgg catagggctc gcggatcgta atctgatagc cccctgccct
     7381 ccggccgaac atatccgtcg ctaaaattct gtgacccccc gtgttcggcg caagataagt
     7441 catctctgca tcccaattaa acaggccatc cccgctgtct gaatcctcaa cgagtccttc
     7501 cgggccaagg gccaccggcc acactaaaag atatggatcg atcaacatcg agctgagatt
     7561 gatattaacc gcctgctcct tggtcatatc aagggaatag acatcaacat cgacgggata
     7621 atcgctcatc ccctgataca cttcgcccac ctttaatgcg tgcccatcat caggatctac
     7681 ataagggatc aaggtttgat tacttttgag cgtataggtt gtcggccact tattatgctg
     7741 acgcacgatg acgaagtgtg ggccgccggt cgttgtggtc aggcgcgctt tctcgactcc
     7801 cccgtaaggc tcatcggaga taacggaatc tatgtcctgg gtatcctgaa taataaggac
     7861 ggcgtcgccg tcaccactca cttcggccgt gatcagagtc ccgatcggca catcaacaac
     7921 gaaagcgcga tgggctaaat tatcggcgat ctcgaacttg tgctgcacat tgccgcgttc
     7981 cggtttggct ccccggaacg cccctgttgg ttcgcccagc atcgtttcca tgcgcggcaa
     8041 tatgtcggcc gccgacgtaa caatgccaaa accaacttcg gtcacccgat ggccggtcaa
     8101 tccgatgatg tcaccctcca tcgtcaccgc aattccaccg ctttgcccac cagccaccgg
     8161 cgaatccact tgcaggtacg tcatgccgaa tgattcctgc tcgcgcaaac gggagacaag
     8221 ggtttgggtg agcgccggac gcggctggcg ggttacatcc ccggggtaac cgatgagcag
     8281 cagcgggcta ccaaccgcaa gctgttcgcc gtcggtgagc gacaggggga cgatgctcgt
     8341 atcgataggg cccagcaagg ccagatcgac cagcaggtca acacccgcca ccggagcaac
     8401 gtgcttctcc ccatcgggaa aggtcacgtt gacttccgtg gccggccaaa cgacgtgagc
     8461 attggtcagg atatatcgat cctcgatcag cacgccagtg ccgaaacggc cgtcggcctc
     8521 gatgcgcgcc acggccggcg acaggttatt aaaaatgtcg gccggagtca gcggggtagg
     8581 ggttggctca ggtgtcgaag ttgatatagg tgtaggaatg ggggtcttgc tgggccgtgg
     8641 agtcgcttcc accccaagcg tcggaatagc agtggcctcg acaacatttt gtggctggtt
     8701 cgtacaggcg tttagcgtca atagcgcgag aatgacagtg acaataaagg tcgacatagc
     8761 ttccccccgg aagtgaagaa caacaatagc aaactctggc ggtttggcct attcataggt
     8821 taacaaccgt gtttctcacg aaacggcttt ctcaagcggc cgaatgtaac ctaacaagag
     8881 aattatagcc gacgcaccaa ccgccgccag caggctccat ccccagggca caaccaccgc
     8941 cccactcgcc agcgccccaa aagacaccgc cgcgccgata gccagcccgc tcagcaacgc
     9001 caccgacagc gcctgcagga agccgaagct gcgccacttg aatgcgggcg ggatggtggc
     9061 attgcgccag cggaagtagt cggccagcga gggatcatcg gcgatgagcc gctccttgat
     9121 ctggttcatg gcccgcacgc tctccagcca ggcctgccgc agccgcgcca gctggagaat
     9181 cgtgatcagt cccaaggccg acaacaggac gaagataatt gcaaacgtta tgtagagttg
     9241 ttgctgggcg gccgtttcaa cctgcgtcga cagcaaagcc gcgatgagcg tggcgaaggt
     9301 gacgaagaaa tattgccacg cccgcgcccg atcctcgttg gcctggaaag cggcctgagc
     9361 gatatagtcg aactcggcgg cgatgatcga ccggctcagc tcggtatcgg gcggcggggc
     9421 gggtgtggtc atgggctaca gtatacaaga agtgggggtg gcaggcagta gatcagtagg
     9481 tcagtaaggc agtagatcag tcgtctgtcg tctgtcgtct gtcgtcggtc gtcgcgcgac
     9541 aggagccgcg atctacccgt gcggataaac caccannnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnngtcgt cggtcgtcgc gcgacaggag ccgcgatcta cccgtgcgga
     9721 taaaccacca cgctcgtcgg cttaatcatc aggataaccc gcgtctcctt gtccttgcgc
     9781 tccagcgggg caacatcgcc atagtaatgc tccacgccat catagagcct ggcgtgatag
     9841 tcgatattgg cattgccctc gtcggggaca atagcctcca cgcagccgcg cacgtcgatc
     9901 cagcgatagg gattggttgg gtcgatggcg gtgacggcca ccagcggatt gtgacgcacg
     9961 ttttcgtatt tgcggcgtcc ctcaaccgtg ttgacaagca catgctcgcc atcccacgaa
    10021 caccagacca cggtgttctc cggctggccg tccctggcca cggttgtgaa cacaacgtag
    10081 accggcccgg tgatcagatc aagatgggtc gaaggaatag tcacttcgtt cccgttctta
    10141 ttataggtag gcatgtcatt cactccttga atggataagg tatatccaga ctatagcaac
    10201 tttagagcca tagggcaggg tggcttttat tcgccttttc cgacaactaa aaacccttcc
    10261 ccgaagcctc gaacgtggtt gactgagaaa ttgtcctgtg ctatgattca tccctaggcc
    10321 attggccttt gttttgttgt caatttttag atcatgaact acggtgtgcc ggagttcgcg
    10381 aaaagagggt agtagattaa gagagtaaga cgtccacaag taaccaaacc tatcaccgtt
    10441 gattaccgga ttaaccggca gatcagagcc cgcgaggtgc gcctgatcga ccacaacggt
    10501 gtgaaccggg gcgttctgcc cttcttcgat gcgttgcgca tcgctgagga tgctgaactg
    10561 gatttggttg aagtggcccc gaacgccaac ccacccgtgt gccgggtgct cgatttcggc
    10621 aagttcgcct acgaacaaac caagaaagag cgcgaagccc gcaagcacca aaagaagatc
    10681 gagatcaaga cgattcgtct ctcgcctcga acgggtgatt accataagga tattggcgtg
    10741 cgtaatgcgc gcaaatggct cgaagagggc aaaaaagtca agttcatggt ccgcttcaaa
    10801 tcccgggaga tttcctaccc cgagatcgga cagaaggcct tgcaagagat cgccgatgac
    10861 ctgagcgata tcgccatcgt cgaacaacaa ccgacgctgg aaggctggcg catgacgctg
    10921 accctctcgc cggagagtgc cgcgcccccc aaggggcagg ccggctccga cgcgcccaaa
    10981 gaagagccgg aagcaaccca aggatagaag aagcccttcg gggctttttt ttcggcggat
    11041 gccggtgatg aggacgagat cgcccggccc gcgacatatt ttgcaacaca gggatgtaaa
    11101 tcatgccaaa gggaacgaag aaatacaaac tgaagacgta caaggcggcc tcgaagcgct
    11161 tccgcgtgac cggcaccggc aagatcgtcc gcaccaaggg cggtaaaagc cacttgcgcc
    11221 ggcgcaaatc gaagcgcacg aagcagcagt tctccgaaat gatcgtggtc accgcgcccg
    11281 gcgacattcg ccggatcaag cgcctggccc cgtatctgaa gaagtacaag gccaatccgc
    11341 cgggctaatt gttttatcgt tgatttgaaa aaatcgctgc ctggcctcgc gccctgcgtt
    11401 gagcgtccag gctaccatga aatgcgaggt gaattgatat gcgtgtaaaa ggtggttttg
    11461 taacccgccg tcgtcataag aagatactcg acatgaccaa gggtcagtgg ggcagccgtt
    11521 ccaagctgtt ccgccgcgcc aacgaggcca tgatgaagtc ctattggtac gcctaccgcg
    11581 accgccgcaa caagaagcgt gatttccgcc gtctgtggat cacgcgcatc aatgcggcca
    11641 cgcggatcca tgggctgtcc tatagtcgct tcatgcacgg tctgaaggtg gccgaggtca
    11701 gactggatcg caaagcattg gccgacctgg ccgtgcgtga cgaggcggcg ttcgcggccg
    11761 ttgttggcgt cgccaagaac gctttggctt gatcccggaa ataccctctc gactattcaa
    11821 acgccacacg ctcgtccgtg tggcgttttt ttattggcaa cggccgcggc gggaaaccgc
    11881 ccatattgcg tagttgcggc tctttccgcc cgccgctatg atcgcccatt agcatgaccc
    11941 ttgatccaac tgcgcgggaa tttgacccgg cgatccgcaa actccgtcaa accggctgga
    12001 ttctgctcat cctgctggcg gccgcggcgc tccgattggt ggcgctgcca tccgcgccgc
    12061 ccggtatgac cggcatacct cctggtatga cccatgatga ggctgaccac ggcctgaccg
    12121 cctgggagat cgtcaatggc gcccgcgcgc tctatttcac cgtcggctat ggccgcgagc
    12181 cgctctatga ctacaccacg gcgctgctca tggccgggac aggaccgacg atcctggccg
    12241 cgcggatgac gtcggtctac ttcagcctgc tgatgatcgc cgccgtctat gcctgggcac
    12301 ggcgggcgtt cggcgcgccg gtggccttgc tcacggccgc ggggctggct gccggcttct
    12361 ggccggtcat ggccgggcgg caggcgctgc gctcgatcgc gctgccggcg ctgttctcct
    12421 tggcggtgtt ggtcttttgg tggggactgc tggaagggga gaaggggaga aagggagaaa
    12481 gggagaaagg gagaaagatc gctccccagc accctttccc cctttctccc ctgctcttcc
    12541 ttgccgccgg cattctcctc ggcctcagca tctacaccta catccccgcg cgggtcatgt
    12601 ggccggcgtt cccggcgctg gcgctttat
//
LOCUS       DAOMQG010000063        12535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2178_length_12535_cov_10.284135, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000063 DAOMQG010000000
VERSION     DAOMQG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_12535_cov_10.284135"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PLC06_05455"
     CDS             complement(<1..1202)
                     /locus_tag="PLC06_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89925.1"
                     /translation="MKKLLILLAALLHLWLAACGRREAPALPADPSPVVPATTAPTLP
                     PATSAAIATTPPPITPTTSAVALPANSLMTGPLVGFYIEDTYEDLYLSIYDAGTGAFR
                     VIQSATPIYIDEAQWFDGGCRLFVHGQVTDLHGAVEWSLPPEVAARINYVNIARLSPA
                     RNYVAHAVAGERGDAADIEIIALSPPYDAVRLATGDGVRAFVWSADGTQLYHTGYDES
                     GVLQVYRASADGAMREQLTAHGADSGGVGAITGLALSPDERYLAYSVQNLLSPSHPYT
                     YQPADEGWVGIIDLVAGTSAAVRPTKFGSAEAGRGLVWDAAGENLLIIGDSLPIAADD
                     PDAGRRLLWVTPDGEVTRALATADGPEGAGGHMGWVAPLGDINTLLVSVLNDFYLYED
                     GAFRRLDAA"
     gene            complement(1217..2029)
                     /locus_tag="PLC06_05460"
     CDS             complement(1217..2029)
                     /locus_tag="PLC06_05460"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HRO89926.1"
                     /translation="MSPCPAGEPEPSPSVTHPSPGVIGIVGGVGPYAGLDLQRKILEQ
                     TVATRDQDHLPVIAVSWPGPIHDRTEYLLGRVAENPAEAMLAQLRLLARAGATVAAIP
                     CNTAHAPAIFDVIRAGVAEFAPSLRLLHMIDETAAHLKARHTALKTVGVLSTTGTWRT
                     RLYPAALEPLGYRVVVPDEAMQEALVHPSIYDPDYGIKATGVVTGQARAELGMAIVTL
                     RNQGAEAVILGCTELPPAFPEREYGGISLIDPTLALARALIRAADPQRLKPW"
     gene            2127..3194
                     /locus_tag="PLC06_05465"
     CDS             2127..3194
                     /locus_tag="PLC06_05465"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRO89927.1"
                     /translation="MEPAVQPADLIIQPIEPAWVERLPNGDHAIHWREPAERVSVQMI
                     DGPDPSATPIATYTFQVASSARSVVCPQDTPRVPDTCRPYFLVDLDGRRHLIAERELP
                     LAGGVNFRDAGGYHTTGGRVVRWGRVFRAGSLAGLTDEDVACLGRLGLRLSCDLRSWE
                     EMIARPDRLPPGAVARHTAVATEVGPVRRVIALFRKRHRIQELLQDAYTVMLDQNGPL
                     FAGIIRAAADPANLPLVIHCTAGKDRTGLAVALLLLTLGVPEETVIADYTLSNRAFDL
                     LSGRMRSEMEPLYRLGFDEARLRPFLLAEARTLTGALAHLRRRYGSVERYLQQAGLDG
                     AAVERVRETLLTTATTDPLTH"
     gene            3241..4773
                     /locus_tag="PLC06_05470"
     CDS             3241..4773
                     /locus_tag="PLC06_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRO89928.1"
                     /translation="MHNPPFRKLLIANRGEIALRVIRACHELGIAAVAVYSDADAGAP
                     WARAADEAWPLHGNSAAETYLDQEKILTIAAACGAEAIHPGYGFLSENAEFAEACARR
                     GVVFVGPSPEAMRALGSKAAARSLAEAHHVPVVPGVDGAGKSDDELAEAAAAIGYPVL
                     IKASAGGGGKGMRVIHEAAELRDGLLAARQEAQSAFGDPHLLLEKYFTKIHHVEVQVL
                     GDKGRDAPHGNLLHLYERECSIQRRHQKIIEESPSPLVGENEALREAITSAAVRLARA
                     AGYVSAGTVEFIVDDAGGFYFLEMNTRLQVEHPVTESVSGVDLVTWQLRIAAGEPLPF
                     AQADIRRRGHAIECRVYAEDPANGFLPSIGTIAHYARPSGPGVRVDDGIESGAVVSPY
                     YDPMLAKIITWGQDRAEAIRKMERALRETVVLGVTTNIGYLRDILATPAFVAGDTGTN
                     FLAQHMAEWRPPVGASEDEWVAAAVYEMVGRTTDDRRPTTGDGRQVYDPWAAAVGWRN
                     VG"
     gene            4849..5121
                     /locus_tag="PLC06_05475"
     CDS             4849..5121
                     /locus_tag="PLC06_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRO89929.1"
                     /translation="MSLNIKNPEVERLLDDIVGMTGESKTEAIRKALDERRRRLQLQS
                     VAPRSEARLHAFLENEIWPQVPPELLGKPITKEEEEAILGYGELGV"
     gene            5118..5504
                     /locus_tag="PLC06_05480"
     CDS             5118..5504
                     /locus_tag="PLC06_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO89930.1"
                     /translation="MILDSSAIVALFFKEPGYESLQHKLVDAEVLAISAATLVETAIV
                     LSARLKTDARGSLARFIEENGVIVIPFTEGHYGIAVTAWLKYGKGRHPAALNFGDCLA
                     YAAAKAAEMPLLCVGDDFPMTDLTLA"
     gene            complement(5507..5917)
                     /locus_tag="PLC06_05485"
     CDS             complement(5507..5917)
                     /locus_tag="PLC06_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRO89931.1"
                     /translation="MNDIALNKIQSIQRCVRRAREEYDASDAFEADHTRQDAAVLNVL
                     LACEQAIALANHVIKTRQLGIPTSSGESFDLLATAGIIDPDLSARLQKMVGFRNAVVH
                     HYQRMDLEIVRSAITAGLDGLVEFGDRIVEYLDA"
     gene            complement(5914..6354)
                     /locus_tag="PLC06_05490"
     CDS             complement(5914..6354)
                     /locus_tag="PLC06_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRO89932.1"
                     /translation="MERISGTFQPEVDKGAIIRHVLAYYPATQGIYLFGFHGMTDEHP
                     HSDIDIALLLLPGEAEQAGRLALSDLHLQLEMALGRDVDLLNARLASTVFQKAIIFGE
                     LVYCADRYAVDEFEMLTLSCYQKLNEERAEILAEFRRTGRAYAV"
     gene            complement(6411..6749)
                     /locus_tag="PLC06_05495"
     CDS             complement(6411..6749)
                     /locus_tag="PLC06_05495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRO89933.1"
                     /translation="MWRGEIWWASLPDPVGSMPGYRRPVLIIQSDTFSASAIKTVAIL
                     SITSNLHLSNSPGNLLITSRQSGLPRDSVVNVSQILTIDKSALTERVGMLPPRQMERI
                     DDGMRLVLGL"
     gene            complement(6749..6985)
                     /locus_tag="PLC06_05500"
     CDS             complement(6749..6985)
                     /locus_tag="PLC06_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89934.1"
                     /translation="MKTAISIPNPIFEEAEGLAKRLGMSRSELYATAVAQFVEAHREE
                     AIIAALNEVYAENDSAVDPVLNQLQWLALPYEEW"
     gene            complement(7017..>7862)
                     /gene="rsmA"
                     /locus_tag="PLC06_05505"
     CDS             complement(7017..>7862)
                     /gene="rsmA"
                     /locus_tag="PLC06_05505"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRO89935.1"
                     /translation="PKQSLGQNFLFDEGLLARIVEAAELSPADAALEVGPGLGALTRQ
                     LARAAGRVVAVELDDRLLPILRYELEPFANVEIVHDDILTFDVGAHFDGPFVVVANVP
                     YYITGAILRRLLEGRPRPKRMVMTVQREVADRLTAKPPRMSLLAVSVQYYGSVRLVGA
                     VKAGAFWPRPEVDSAVICIDVERRESTDGTDFTDEDSLQSVLSVKSVDSSSASDESFF
                     RVVRAGFGEKRKQLKNNLRRLGLSDEAISVALESAGIDGRRRAETLGVGEWEQLVASL
                     PHIPD"
     assembly_gap    7863..7962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7963..10061)
                     /locus_tag="PLC06_05510"
     CDS             complement(<7963..10061)
                     /locus_tag="PLC06_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine carboxypeptidase"
                     /protein_id="HRO89936.1"
                     /translation="MKKILVIYAGEGGEISDHLFMGHELTLQRAGCGGDPERARALIQ
                     ENDGKVDAIALEGLPMSLELGAARRPHETGATLAPVAQQTLVVDGSGIRAGLERWGVI
                     LADRAQPGIFAQKRVLMVPGLNHNGLAQALGRRAADLRYADPIVYFSLPALPLVGSRG
                     TLEQAAGPTLDQLKDAAFDSLYPLPGGPAEPRDAAAFEWADILAGDIGAIRRYAPAEL
                     KRKTVVVNWATEEDLADLRERGVNIAVTMMPALDGKGELGRWSAAVIEAIMVALRPDP
                     NAPLSEDTYLDLMASLDWTPAIRYLQPGEAGINRFAFVIHPLSIKFIHNDPRFRWTKV
                     LPDDLVESFSARMPPMYISRITGGVSPTTGQRIEGHLITLGATPRQMMSRGERFTYDK
                     LNQAARIAERKGARIMGLGAFTSVVGDAGITVAHETDIAITSGNSLTVAATLEAAKQA
                     VIKMGAADLTKGKVMIIGATGSIGAVCARLLAQAIYDVVLVSIEPERLIELKRTIHEE
                     TPGAHVAIATRADEYLGECDLIVTATSAFGQRIVDITKCKPGTVICDVARPPDISKEE
                     AALRPDVLVIESGEVLIPGDIDFGYDIGLPPRTAYACLAETALLAMEGRFEDYTLGRN
                     ITMERVKEIYRLFKKHEFQIAGLRSHDEYVTDGMVAEKRKLADQLRADREMFARVQSE
                     SAAKLATIPVSSKGVKA"
     gene            10374..12431
                     /locus_tag="PLC06_05515"
     CDS             10374..12431
                     /locus_tag="PLC06_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO89937.1"
                     /translation="MSQKKVGLIMALLLVVALALVACQPQSQTVEVTRVVTETVVEEG
                     QSVVVTRIVTEVQQVVATPAPVQTGPNVPSDLTVWREQSFGEPETLDPALDYETSGGN
                     VIQNVMEPLVIYDHTSSTDMVPALAVEVPSLENGGISADGKTYTFNVREGVTFHNGGT
                     LEPHDVAYTFQRGLLQSDPDSPQWLLIGPILGYTSGDVTEGIQNGVFASDPEGLKTNA
                     TPEELLATCELVKAAVVADDDAGTVTFNLAQSWGPFLATISQTWGAILDQEWAIEQGA
                     WDGDCATWQNWYAPGAENSELTNIINGTGPYMFDHWTPGEELVLTAYEGYWRTAETPR
                     WEGGPYGPATIKTVVYSVVDEWGTRIAALQAGDAEWVVVNPEHEVQVDSFVGEICDSQ
                     TGECVPNEANPDGFLRKWVNRPSVSRTDIFPTFAIAEGSPYVGSGQLDGSGIPLDFFS
                     DVNVRKALATCFDYETYNQEVLLGQGSRNNGPIISGMLGYNPDGPMYEYDPEACAGYL
                     AEAWGGALPDTGFRFQIAFNTGNTTRQIVGEILQAEMSAINDLYQVDTLGLPWPTLLA
                     AQRVSMLPVSINGWQEDIHDPHNWVQPYLVGIFGSQQNIPAELMDQYREFVNNGVQAS
                     DPAEREQIYFDLQELYYETVPQVILLQRDSPWYSQRWVDGYYYRIGAFGRDYYALSLT
                     AAQ"
BASE COUNT         2248 a         3971 c         3986 g         2230 t
ORIGIN      
        1 gccgcgtcca gccgccggaa cgcgccatcc tcatagaggt agaagtcgtt cagcacgctc
       61 accagcaggg tattgatgtc gccgagcggc gcgacccagc ccatgtggcc gcccgcgcct
      121 tcggggccgt cggccgtcgc cagcgcgcgg gtgacctcgc cgtcgggtgt gacccacagc
      181 agccgccgcc cggcgtcggg gtcgtcggcg gcgatgggca gactgtcgcc gatgatgagc
      241 aaattctctc ccgcggcgtc ccacaccagg ccgcgccccg cctcggcgct gccgaacttc
      301 gtgggccgga cggccgcgct cgtgcccgcg acgaggtcga tgatccccac ccatccctcg
      361 tcggctggtt ggtaggtgta gggatggctg ggcgagagca ggttctgcac gctgtaggcc
      421 agatagcgct cgtcgggcga gagagctaac ccggtgatgg cgccgacgcc ccccgaatcg
      481 gccccatgcg cggtcagctg ctcgcgcatc gctccgtcgg ccgaggcacg atagacctgc
      541 aaaaccccgc tctcgtcgta gcccgtgtgg tagagctgcg tcccgtcggc cgaccagacg
      601 aaggcccgca ccccgtcgcc ggtcgccagc cgaactgcat cgtaaggcgg ggaaagggcg
      661 atgatctcga tatcggccgc gtcgccccgc tcaccggcaa cggcatgggc aacatagttc
      721 cgagctggag agaggcgtgc gatgttgacg tagttgatgc gcgcggccac ttccggcggc
      781 aagctccact caaccgcgcc atgcaaatcc gtcacttgcc cgtggacgaa cagccggcac
      841 cccccgtcga accattgcgc ctcgtcgatg tagatgggcg tagcgctctg gatcacgcga
      901 aacgcgcccg ttccggcgtc gtagatcgac agatagaggt cctcgtaggt atcctcgata
      961 tagaacccaa ccagcgggcc ggtcatcaac gagttggccg gcagcgcgac ggcagaggtt
     1021 gttggcgtga tgggtggcgg cgttgtcgca atggcggccg acgtggccgg tggcagggtt
     1081 ggtgcggtcg tggccggcac aacaggcgac ggatcggcgg gcaaggcggg cgcttcccga
     1141 cggccgcacg ccgcgagcca gagatgcagg agagcggcca gcaggatgag cagtttcttc
     1201 atgcggatat tcataatcac cagggtttca gtcgttgcgg gtcggccgcc cgaatgagcg
     1261 cccgcgccag cgccagcgtg gggtcgatca atgaaatccc gccatattcc cgctccggga
     1321 acgccggcgg cagctccgtg cagcccagga tgaccgcctc ggccccctga ttgcgcagcg
     1381 tcacgatggc catgcccagc tcggcccgcg cttgccccgt caccacgccg gtggctttga
     1441 tgccgtagtc agggtcgtag atcgacggat ggacgagcgc ttcctgcatc gcctcgtcgg
     1501 gaacgaccac gcgatagccc agcggctcca gcgctgccgg gtagagcctc gtccgccacg
     1561 tgccggtcgt ggacagcacg ccgacggtct tcagcgccgt gtggcgagct ttcagatgcg
     1621 cggccgtctc gtcgatcatg tgcagcaagc gcagcgacgg cgcaaactcc gccaccccgg
     1681 cgcgaatgac gtcgaagatg gccggagcgt gggccgtgtt gcaagggatt gcggccacgg
     1741 tcgccccggc gcgggccagc agccggagtt gcgccagcat cgcttcggcc gggttctcgg
     1801 ccacgcggcc gagcaggtat tccgtccggt catggatcgg ccccggccag gagacggcga
     1861 tcaccggcaa gtgatcctga tcgcgcgtag ccacagtctg ctccagaatc ttgcgctgca
     1921 gatccagtcc ggcgtaaggg ccgacgccgc cgacgatgcc gatgacgcct ggcgaaggat
     1981 gagtaacaga tggggaaggt tccggctccc ccgccgggca aggtgacatt ttagggttgc
     2041 tcgattaaac tcaatgaatc agccatcaag cctaacacgt ccaatccggc gcaacaaatc
     2101 tcgccgcgcg actcgctcgt aatctcatgg aacctgccgt tcaacccgcc gacctgatca
     2161 ttcagccaat cgaaccggcc tgggtggagc gcctgcccaa tggcgaccat gccatccatt
     2221 ggcgcgaacc ggctgagcgc gtatcggttc agatgatcga tggccccgac ccgtccgcca
     2281 cgcccattgc gacttacaca tttcaggtgg cgagttcggc gcggtcggtc gtctgccctc
     2341 aggacacgcc gcgtgtgccc gacacatgcc gtccttactt tctggtcgat ctcgacggcc
     2401 gccgccatct catcgccgag cgcgaactgc ccctggccgg cggcgtcaac ttccgcgacg
     2461 cgggcggcta tcacacgacc ggcggccgcg tcgtgcgctg ggggcgggtc tttcgcgccg
     2521 ggtcgctggc cgggctgacc gatgaggacg tcgcctgcct gggccgcctc ggcctgcggc
     2581 tgtcgtgcga cctgcgctcg tgggaagaaa tgatcgcgcg ccccgaccgg ttgccgccgg
     2641 gcgcagtcgc ccgccatacg gccgtcgcca ccgaggtcgg gccggtgcgc cgcgtcatcg
     2701 ccctcttccg caagcgccac cgcatccagg agctgctgca ggacgcctac accgtcatgc
     2761 tcgaccagaa cggccccctc ttcgccggta tcatccgcgc cgcggccgat ccggccaacc
     2821 tgccgctggt catccactgc acggcgggca aggaccgcac cggcctggcc gtggcgctgc
     2881 tgctgctgac gctgggcgtg cccgaagaga cggtcatcgc cgactacaca ttgagcaatc
     2941 gcgccttcga tctgctgtcg gggcggatgc ggtcggagat ggagccgctc taccggctgg
     3001 gcttcgacga ggcgaggctg cggccgtttc tgctggccga ggcgcggacg ctgaccgggg
     3061 cgctggccca tctgcggcgg cgctatggct cggtggagcg atatttgcag caggcggggc
     3121 tggacggggc ggcggtagag agagtgcgag agacgttgct gacgaccgcg accactgacc
     3181 cactgaccca ctgacaatcc gtatttgcca cttccccccg gccacccgat aatcacgctc
     3241 atgcataacc caccgtttcg caaactcctc atcgccaacc ggggcgagat cgccctgcgg
     3301 gtgattcgcg cctgtcacga gctgggcatc gccgccgtgg ccgtctattc cgacgccgac
     3361 gcgggcgcgc cgtgggcgcg cgcggccgac gaggcctggc cgctccacgg caacagcgcg
     3421 gccgagacct acctcgatca ggaaaaaatc ctgaccatcg ccgccgcctg cggagccgag
     3481 gccatccacc ccggctacgg cttcctgagc gagaacgcgg agtttgccga ggcttgcgcc
     3541 cggcgcggcg tggtgttcgt cgggccgtcg ccggaggcca tgcgcgccct gggcagcaag
     3601 gccgccgctc gctcgttggc cgaggcccat catgtgccgg tagtccccgg cgtcgatggg
     3661 gccggcaaga gcgacgacga gttggccgaa gcggccgcgg ccatcggcta cccggtgctg
     3721 atcaaggcca gcgcgggcgg cggcggcaag gggatgcgcg tgatccacga ggcggccgaa
     3781 ctgcgcgacg ggctgctggc cgcccggcaa gaggcgcaat cggccttcgg cgacccccac
     3841 ctgctgctgg agaaatactt caccaaaatc caccacgtcg aggtgcaggt gctgggcgac
     3901 aaggggcgtg acgccccaca cggcaacctg ctgcatctct acgagcgcga gtgctccatc
     3961 cagcgccgcc accagaagat catcgaggaa agcccgtcgc cgctggtggg cgagaacgag
     4021 gcgctgcgcg aagccatcac atccgcggcg gtgcggctgg cgcgggcggc gggctacgtg
     4081 agcgccggga cggtggagtt catcgtcgac gacgcgggcg gcttctactt tctggagatg
     4141 aacacccgct tgcaggtgga gcacccggtg acggagagcg tgagcggcgt cgatctggtc
     4201 acgtggcagt tgcgcatcgc cgccggcgaa ccgctgccgt tcgcccaggc cgacatccgg
     4261 cggcgcggcc atgctatcga gtgccgcgtc tacgccgagg acccggccaa cggcttcctg
     4321 ccgtcgatcg gcacgatcgc ccactacgcg cggccgtccg gccccggcgt gcgcgtcgat
     4381 gacggcatcg agagcggcgc ggtcgtgtcc ccctactacg atccgatgct ggccaagatc
     4441 atcacctggg ggcaggatcg cgccgaggcc atccgcaaga tggagcgcgc cctgcgcgag
     4501 acggtggtgc tgggggtgac gaccaacatc ggctatctgc gcgacattct ggccacgccg
     4561 gcgttcgtcg cgggcgacac cggcaccaac ttcctggccc aacacatggc cgagtggcgg
     4621 ccgccggtgg gcgcgagcga ggatgagtgg gtggcggcgg cggtgtatga gatggtgggg
     4681 aggacgacgg atgaccgacg accgacgacc ggcgacggcc gacaggtcta tgacccgtgg
     4741 gcggcggcgg tggggtggcg gaatgtgggg tgagaactgg taaagaaatg aggaattatt
     4801 gatgaatggt agaatgtcgg gtaatctact gcaatagagg tgttgaagat gtccttgaat
     4861 atcaaaaacc ctgaagtgga aagattgctg gacgatattg tgggcatgac gggcgaatcc
     4921 aagacggaag caatacgcaa ggcgctcgac gaacggcgcc gccggttgca gctccagtcc
     4981 gttgctccac gcagcgaagc gcggctgcat gccttcctcg aaaacgaaat atggccccag
     5041 gtgccgccgg aattgctcgg caagccgatc acgaaagaag aggaagaggc catcctcggc
     5101 tacggggagc tgggcgtatg attctggatt cctcggcaat cgtcgcgcta ttctttaagg
     5161 agccggggta tgaaagcctt caacataagc tcgttgatgc cgaagtgctt gccatcagcg
     5221 ccgccacgct cgtggagaca gcaattgtcc tgtcagcgag attgaagacg gacgcaaggg
     5281 ggagcctggc tcgttttatc gaggaaaatg gggtgatcgt gatcccgttt acagagggac
     5341 attacgggat tgccgtgacg gcctggctaa aatatggcaa aggccgccat ccggccgcgc
     5401 tcaattttgg ggactgcctg gcctatgctg ctgccaaggc ggccgagatg ccgttgttgt
     5461 gcgtcgggga tgattttcca atgactgatt tgacgttagc ctgatgctat gcgtccaaat
     5521 actccacgat cctgtcccca aactccacca aaccatccag ccccgccgta attgccgaac
     5581 gaactatttc caaatccatc cgttgatagt gatggacaac cgcattacga aagccgacca
     5641 ttttttgcag tcgcgcgctc aagtcagggt cgatgatccc ggccgtcgcc aacaggtcga
     5701 aactctcgcc gctggacgtg gggatgccca gttgccgggt cttgatgacg tgattggcca
     5761 gggcgatggc ttgctcgcag gccagcagga cgttgagcac ggccgcgtcc tgtcgggtgt
     5821 ggtcagcctc gaaggcatcc gaggcgtcgt attcttcacg cgctcgccga acgcagcgct
     5881 ggatactttg aatcttgtta agggcgatat cattcatacc gcatacgctc gccctgtccg
     5941 gcggaattcg gccaggatct ccgcccgttc ctcgttcagt ttctggtaac aggacagggt
     6001 cagcatctcg aactcatcca cggcatagcg atcggcacag tagaccaatt caccgaaaat
     6061 aatcgccttt tgaaaaacgg tggaggccag acgcgcgttc aacaggtcta cgtcgcggcc
     6121 gagggccatc tccagttgga gatgcagatc gctcagggcc agccgcccgg cctgttcggc
     6181 ctcacccggc aacagaagca gggcgatatc gatgtcactg tggggatgct cgtcggtcat
     6241 gccgtgaaaa ccgaagaggt aaatgccttg cgtggccgga tagtaagcca gaacatgccg
     6301 aatgattgcg cccttatcga cttcgggctg gaatgtgccg gatattctct ccattatact
     6361 cttgccgtaa ggcgcttgca gctttcaggt tgttgctgaa tcaatgccgt ctacagcccc
     6421 aacaccagcc gcatcccgtc atcgatccgt tccatctgtc gcggcggcaa cattcccact
     6481 ctttcagtta atgctgattt atcgatagtg aggatttgtg acacgttgac gaccgagtcg
     6541 cgaggcaaac cgctttgcct cgatgtgatg agcaagttgc cgggcgaatt ggacagatga
     6601 aggttggaag tgatggacaa tatcgcgact gtcttgatag cgctggcgct gaaggtgtcg
     6661 gactggatga tcaataccgg ccgtctgtag ccgggcatcg atcccaccgg atcgggcagc
     6721 gatgcccacc atatctcgcc ccgccacatc accattcctc atagggtagg gccagccatt
     6781 ggagttggtt cagaaccgga tcaactgccg aatcgttttc agcatagacc tcattgagcg
     6841 cggcgatgat cgcctcctct cgatgcgcct ctacgaattg ggcaacggcc gtagcatata
     6901 attcactgcg cgacatgccc agtcgcttgg ctaatccttc agcctcttca aaaatggggt
     6961 ttggtatgga aatggcagtc ttcatgtcag gagtataact ctggttatac cagctgtcaa
     7021 tccggtatgt ggggcagtga tgcaaccaat tgctcccact cccccacccc cagcgtctcc
     7081 gcccgccgcc ttccatcgat ccccgccgat tccagcgcga ccgagatcgc ctcatcactc
     7141 aaccccaacc gtcgcagatt attcttcaac tgcttccgct tctcgccgaa cccggcccgc
     7201 acgacgcgaa agaaggattc atcggaagcg gaagaggaat ccacagattt cacagataac
     7261 acagattgga gagagtcctc atctgtgaaa tctgtgccat ctgtggattc tcttcgttca
     7321 acgtcaatac agatcaccgc cgaatccacc tccggccgcg gccagaacgc ccccgccttc
     7381 accgcgccga cgaggcgcac gcttccatag tattgcacgc tgacggccag aaggctcatg
     7441 cgcggcggtt tggctgtcag gcggtcggcc acctcgcgct gtacggtcat gaccatgcgc
     7501 ttcggccgcg gccgcccctc cagcaggcgg cgcaggatgg ccccggtgat gtagtagggc
     7561 acgttggcga cgacgacgaa cgggccgtcg aagtgggcgc cgacgtcgaa ggtcagaatg
     7621 tcgtcatgga cgatctcgac attggcgaac ggctccagct cgtagcgcaa gatgggcagc
     7681 aggcggtcgt ccagctccac ggccacgacg cgcccggcgg cccgcgccag ttggcgggtc
     7741 agcgcgccca gccccggccc gacctccagc gcggcatcag cgggcgacaa ctcggccgcc
     7801 tcgacgatgc gcgccagcag cccttcgtcg aacaggaagt tctggccgag gctttgcttg
     7861 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttggcctt cacgcccttg
     7981 gacgagacgg gaatcgtcgc cagcttggcc gccgattcgc tctgcacacg ggcaaacatc
     8041 tcccgatcgg cccgcagttg gtcggccagc ttgcgtttct cggccaccat cccgtcggtc
     8101 acatactcgt cgtgggaacg cagcccggcg atctggaact cgtgtttctt gaacaggcga
     8161 tagatctcct tgacgcgctc catggtgatg ttgcggccga gggtgtaatc ctcgaagcgc
     8221 ccttccatcg ccagcagggc cgtctcggcc aggcaggcgt aggcggtcct gggcggcagg
     8281 ccaatgtcgt agccgaagtc gatgtcgccg gggatgagca cctcgccgct ctcgatgacc
     8341 agcacgtcgg ggcgcagagc tgcctcttcc ttgctgatgt cgggcgggcg ggccacgtca
     8401 cagatgaccg tgccgggctt gcatttggtg atgtcgacga tgcgctggcc gaaggccgag
     8461 gtggcggtca cgatcaggtc gcattcgccc aggtactcgt cggcgcgggt ggcgatagcc
     8521 acatgcgcgc ccggcgtctc ctcgtggatg gtgcgcttta gctcgatcag ccgctccggc
     8581 tcgatcgaca ccagcaccac gtcataaatg gcctgggcca gcagccgggc gcaaaccgcg
     8641 ccgatggagc cggtcgcgcc gatgatcatc accttgccct tggtcaggtc ggccgcgccc
     8701 atcttgatca ccgcctgctt ggccgcttcc agcgtggcgg ctacggtcag gctgttgccg
     8761 ctggtgatgg cgatgtcggt ctcatgggcc acggtgatgc cggcatcgcc gacgacactg
     8821 gtgaacgcgc cgaggcccat gatgcgcgcc cccttgcgct cggcgatgcg cgccgcctgg
     8881 ttgagcttgt cgtaggtgaa acgctcgccg cgggacatca tctggcgcgg cgtggccccc
     8941 agggtaatga gatgaccctc gatgcgctgg ccggtcgtgg gcgagacgcc gccggtgatg
     9001 cgcgagatgt acatgggcgg catgcgcgcc gagaaggact cgaccagatc gtcgggcagc
     9061 accttcgtcc agcggaagcg cgggtcgttg tggatgaact tgatgcttag cgggtggatg
     9121 acgaaggcga agcgattgat gcccgcctcg cccggttgca ggtagcggat ggcgggcgtc
     9181 cagtcgaggc tggccatcag gtcgaggtaa gtgtcctcgc tgagcggcgc gttggggtcg
     9241 gggcgaagcg ccaccatgat cgcctcgatg accgccgccg accagcggcc gagttctccc
     9301 ttgccgtcga gcgcgggcat catcgtgacg gcgatattca caccgcgctc gcgcaggtcg
     9361 gccaggtctt cctcggtggc ccagttgacg accaccgtct tgcgcttcag ctcggccgga
     9421 gcatagcgcc ggatcgcgcc gatgtcgccg gccagaatgt cggcccattc gaacgcggcc
     9481 gcgtcgcgcg gctcggccgg gccgcccggc agggggtaga ggctgtcgaa ggcggcgtcc
     9541 ttgagctggt cgagcgtcgg cccggcggcc tgttccagcg tgccgcgact gccgacaagg
     9601 ggcaaggcag gcaggctgaa ataaacaatg ggatcggcgt agcgcagatc ggccgcgcgc
     9661 cgccccagcg cctgggccag cccgttgtgg ttaagccccg gaaccatgag gacgcgcttc
     9721 tgggcaaaga tgcccggctg ggcacggtcg gccaggatga cgccccagcg ctccagcccg
     9781 gcgcggatgc cggagccgtc gacgacgagg gtttgttggg ccaccggggc cagcgtcgcg
     9841 ccggtctcgt gcggccgccg ggccgcgccc agttccagcg acatcggcag gccttccagg
     9901 gcgatggcgt cgaccttgcc gtcattttcc tggatgagcg cccgtgctct ttcgggatcg
     9961 ccgccgcaac cggcccgctg aagcgtcagt tcatggccca tgaacaaatg gtcagatatt
    10021 tcaccgcctt ccccagcgta gataacaaga atttttttca ttttgcttcc cctgatggtg
    10081 ctcatggtga ctattctgaa agaacccgta ttgtcaccat attgacacct aattaacact
    10141 attgtatact gcccgataga tcgtttccac acgttcgtca ttgagccgtg ctgtgctcta
    10201 cctcccccaa cgtcgccgga ccacaaagtg gtttgtgata tggctcgcgc ggggcggcca
    10261 gcatgtctgg agatgacgta ttttcgttaa tttccactac tttaccacaa gcagcaccga
    10321 ataccgccga acgtttagca cggaatccaa cattatccga gaggagtttg aaaatgtcac
    10381 aaaagaaggt tggcctgata atggccttgt tattagtggt tgccctggcg ctggttgcct
    10441 gccagccaca gagccagacc gtagaagtaa cccgcgtggt caccgagacg gttgtcgaag
    10501 aaggacaatc tgtcgtggtc acgcgcatcg tgaccgaggt tcagcaggtg gtcgccacgc
    10561 ctgccccggt gcagacaggc cccaacgtcc ccagcgatct caccgtctgg cgcgaacagt
    10621 cgttcggcga gccggagacg ctcgaccctg cgctggatta tgaaacctcc ggcggcaacg
    10681 tgatccagaa cgtgatggag ccgctggtca tctatgacca caccagctcg accgacatgg
    10741 tcccggcgct ggccgtcgag gttccttcgc tggagaacgg cggtatctcc gcggacggca
    10801 agacctatac ctttaacgtc cgcgagggcg ttaccttcca taatggcggc actcttgagc
    10861 cccacgatgt ggcctacacc ttccagcgcg gcctgctgca aagcgacccc gacagcccgc
    10921 agtggctgct gatagggccg atcctgggtt atacttccgg cgatgtcacc gagggtatcc
    10981 agaatggtgt ctttgccagc gatcccgaag gactgaagac caacgccacg ccggaagaac
    11041 tgctggctac ctgtgagttg gttaaggcgg cggtcgtcgc cgacgacgac gccggcaccg
    11101 tgaccttcaa cctggctcag tcatgggggc cgttcctggc caccatctcc caaacctggg
    11161 gcgcgattct ggatcaggaa tgggccattg agcagggggc ctgggacggc gactgcgcca
    11221 cctggcagaa ctggtacgct cccggcgcgg aaaactccga actgaccaat atcatcaacg
    11281 gcaccggccc gtatatgttt gaccattgga cccctggcga agaactggtc ctgacggcct
    11341 atgaaggcta ctggcgcacc gcggaaaccc cgcgttggga aggtggcccc tacggcccgg
    11401 caaccatcaa gacggtcgtc tactccgtcg tcgatgagtg gggcactcgc atcgccgcgc
    11461 tgcaggccgg cgacgccgaa tgggtggtgg tgaacccgga gcatgaggtc caggttgatt
    11521 ccttcgtggg cgagatctgc gattcgcaga ccggcgagtg tgtgccgaac gaagcgaacc
    11581 ccgacggctt tctgcgcaag tgggtcaatc ggcccagcgt gagccgcacc gacatcttcc
    11641 cgacgttcgc catcgccgaa ggctcgccct acgtgggcag cggccagctg gacggcagcg
    11701 gcatcccgtt ggacttcttc agcgatgtca acgtccgcaa ggcgctggca acctgcttcg
    11761 attacgagac ctacaatcag gaagtgttgt tgggccaggg ttcccgcaat aacggcccga
    11821 tcatcagtgg catgctgggc tataaccccg acggcccgat gtatgagtac gatcccgagg
    11881 cttgtgcggg ctatctggcc gaggcatggg gtggcgcgtt gccggatacc ggcttccgct
    11941 tccagatcgc cttcaatacc ggcaacacca cccgccagat cgtcggcgag attctgcagg
    12001 ccgagatgag cgccatcaac gacctgtatc aggtggatac gttgggcctg ccgtggccga
    12061 ccctgctggc cgcacagcgc gtgtccatgc tgcccgtgag catcaatggt tggcaggaag
    12121 acatccacga cccgcacaac tgggtgcagc cgtatctcgt cggcatcttt ggctcgcagc
    12181 agaatatccc ggccgagcta atggaccagt accgtgagtt cgtcaataat ggcgtgcagg
    12241 cctccgatcc ggccgagcgc gagcagattt acttcgatct gcaagagctg tattacgaga
    12301 ccgtgccgca ggtcatcctg ctgcagcgcg acagcccgtg gtactcccag cgctgggtcg
    12361 acggctatta ctaccgcatc ggcgcgtttg gtcgtgatta ttacgccctt tcgctgacag
    12421 cggcccagta agcccgccca cccgtctatc gatcgacgac agcgcgtaac tgcgccgtca
    12481 tcgcgaagcc cctctctccg gtcaggccga agagaggggc ttttttagtg acgag
//
LOCUS       DAOMQG010000064        12498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2184_length_12498_cov_11.006108, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000064 DAOMQG010000000
VERSION     DAOMQG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12498
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2184_length_12498_cov_11.006108"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1903
                     /locus_tag="PLC06_05520"
     CDS             263..1903
                     /locus_tag="PLC06_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRO89938.1"
                     /translation="MSQYAHGGELVARSLAAQGVRTIYTLCGGHVAPIYDGCLNHDIA
                     VIDFRHEQAAAHAADAHARLTRNIGVALVTAGPGVTDAVTGVANAWQARSPMILLGGA
                     APLKTKGMGALQEMPQVEMFRTFTKAAFTIERTEDIPERMAEAYHAALSGRPGPVFIE
                     LPFDVLFNAVDSPERMPDRVTIDPVAPPVDAVQAMFDLLRAAERPLIIAGTQVYWDGA
                     SDALRELTDQTAIPVFTNGAGRGTLSMDHPHCFKATRGKALRAADVVLLIGTPLDFRL
                     KYGREDWNPDSKLIQIENDAAELNHNRTADVALTADARLVLEALAEGLQGIRFQPWLD
                     QVAEWERARNEKQAAWEALEDAPVNHFRFAAEVNRFVDGNTIVIGDGGDIVSACAKVL
                     KVTQPGQWLDPGPLGCLGVGAPFAVAAQHLYPDRRVLIISGDGSFGLNGFEFDTAARF
                     GLPIVAIVGNDAGWGQIRGPQVQMVGAERAIATSLAPTRYDKVVEALGGYGELVEDPD
                     GVLPALERAFASGLPACINVMLDPAGMSKTGASSPYIV"
     gene            complement(1924..2331)
                     /locus_tag="PLC06_05525"
     CDS             complement(1924..2331)
                     /locus_tag="PLC06_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRO89939.1"
                     /translation="MSSFVLDTHSLLWYLEGNRRLGSRAQAKISAVESHLVVPIIVLA
                     EAGIIIGHGRTKIPSVAYLIDSLAKDHRFEIHPLTLDIFKLSLTSEASRITELHDRLI
                     VATALYIQSLGLDVSLVTRDEDLTNARLVPIVW"
     gene            complement(2324..2560)
                     /locus_tag="PLC06_05530"
     CDS             complement(2324..2560)
                     /locus_tag="PLC06_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89940.1"
                     /translation="MIIDNRDLEALLERISLLPPEYRVRLMHGILETLIPSPLKKTET
                     LQFGEFRDFDGPFSTIEDYVIAEWRPSERELSGE"
     gene            complement(2653..3945)
                     /locus_tag="PLC06_05535"
     CDS             complement(2653..3945)
                     /locus_tag="PLC06_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89941.1"
                     /translation="MAHITIVTTGSRGDVQPFIALGLGLTANGHTVRLATHTLYADWI
                     QSNGLGFSPVEGDPMEAVQGRQGRDWMETGRRGLGFVRGFRELIGPILHQATADVLAA
                     CDGTDLILFSGPAFYTAYNVAEKLGKPFIQSYLQPVNPTREFPSAIFPTRHKGGALFN
                     YATHVIGGQLFWQMIRPIMNDVRRDLLKLRPFSFGGPFLDMLRRRLPVVHGYSPTVLP
                     KPHDWNESAFVTGFWFQDDPAYTPSEALATFLASGEPPVYVGFGSMTGNDPERLTAVT
                     LEALRLSGRRGVLLSGWAGLAEGDLPDSVLRLDAVPHDWLFPHMAAVVHHGGVGTTHV
                     GLRAGVPNVVVPFFGDQPFWGDRVYELGAGPRPISQSELTAGRLAAAIETATSDEAMR
                     SRAAEMGRRIQAERGVEEAVAIVNRYLAQRPAFFEGIM"
     gene            4056..4856
                     /locus_tag="PLC06_05540"
     CDS             4056..4856
                     /locus_tag="PLC06_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012153695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO89942.1"
                     /translation="MDFKDNVVILTGASTGIGEALAHALAKQGAWLVLAARNAQKLEA
                     VAAECRARGGRALVVATDVTDEDECRELIERAAAEYGRIDTLINNAGLSMWMKLEDVE
                     DLSTLEYLMRVNYFGSMYCTYYALPWLKQSKGRIVAIASVAARTGVPTRTGYSASKHA
                     MVGFFESLRIEVEDDGVSVTIAYPDFVASGMHTRSLGADGKPLGHNPLQLDKIMTSET
                     CARLIIEGAAARKREIVMSNRARFGQWLKLIAPGRVDRIAKKAIEKGR"
     gene            complement(4930..5703)
                     /locus_tag="PLC06_05545"
     CDS             complement(4930..5703)
                     /locus_tag="PLC06_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRO89943.1"
                     /translation="MKKLTVYLLLSLIAAFALVGCGVLQEPEAASGPIEAIPLETQSA
                     GVAPVEATETSADPASTETTADEPTAEAPAEAPAAGARVFTIDSAASEVRFQLDEDLR
                     GERKTVIGTTDQVAGQLSLNLADLSQTEVGIIQINARTLLTDNNFRNRAIQNEILDTG
                     QFEFITFTPTGVEGLPATAAVGDTVTFTLTGDLTIRDITLPATFTVEATAVSDTQITG
                     SATAIVNRVDYDLQIPSVPNVANVEEDVELYIDFTANAS"
     gene            complement(5823..7184)
                     /locus_tag="PLC06_05550"
     CDS             complement(5823..7184)
                     /locus_tag="PLC06_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRO89944.1"
                     /translation="MRSLSLKLTLAFLIVGLTGVVLVSVFVGRMTRREFDRFVVDRYH
                     DDVLAEVTEYYARNGSWDNLRGALRWRRGPGGAAVNGGMIPLISLTDSDGLVVFSTIP
                     GDAIGRRSAIGDDGDQLPVEVDGDVVGYLRFADRSSARDVRGSPEARFLTAVDRAVRW
                     SAAGAAVLALLLGIILAGTISRPVRELTEATKVLAGGDLGHQVPVRTSDEIGELAHSF
                     NQMSADLAHSNQLRRQMTADIAHDLRTPLSVILGYSEALADGKLPGGPETYEAIHRQA
                     QHLNRLIEDLRTLSLADAGQLSLVRRPVEPRGLLEHTALAYLPAAEARQIALLVEAAD
                     VPPILVDPDRILQVLGNLVSNALRHTPDGGSISLSATQHDDQVLLRVRDTGSGIPAEE
                     LPQIFDRFYRGDMARTSDGSSGLGLAIARSLIGAHGGSISAGNVAGGAQFTIALPMSA
                     HGI"
     gene            complement(7189..7890)
                     /locus_tag="PLC06_05555"
     CDS             complement(7189..7890)
                     /locus_tag="PLC06_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO89945.1"
                     /translation="MSKTILVIDDAESLRMLVRSYLVQEGFRVVTAANGREGLFVARQ
                     ERPDLIILDLMMPELGGFDFIKAYAREGDAPIIILTARIEESDKVLGLELGADDYLTK
                     PFSMRELAARVRAVLRRLDKGGGVGPSALLRAGDLTLDRDRREVTVGQASVDLTPSEF
                     DLLATFLSSPGRVFSRLELLDKLQGDAYEGYERTIDVHIRNLRAKIESDPRNPRYIVT
                     VYGIGYRLAAEPIPA"
     gene            complement(8119..8862)
                     /locus_tag="PLC06_05560"
     CDS             complement(8119..8862)
                     /locus_tag="PLC06_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89946.1"
                     /translation="MDRSKKWSLLVIAGVLALMLGLLGSIALAQGSTPEAEVPAQESE
                     VTPETKGWFGFGHRGGFGSRFGDNTNWLENLAEALGITVGELEDAQERAFAASVADAV
                     TAGQITQEQADQILAQHALKSYIDRQAILATALGMSVDDLKAALADGKSIVDLMVEQG
                     IDSTTLQANAKAAYEAAVQQAVTDGVITQAQADEILAGNGLNLFGRGGFGGGHRGGGR
                     GGRGGFHGFGGQSSPNEGGAETTDTGFDA"
     gene            complement(9082..11247)
                     /gene="ppk1"
                     /locus_tag="PLC06_05565"
     CDS             complement(9082..11247)
                     /gene="ppk1"
                     /locus_tag="PLC06_05565"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRO89947.1"
                     /translation="MVEITKPEPVQETRKPGNAKVAAKPPLVDLRDPSLYINRELSSI
                     EFNRRVLHETQSNHPLLERVKFLAIFHSNMDEFFMVRVSGLKQQFKLGLTTVSSDGLL
                     PREQLATIHRTLTQLFSEANSIWRDHLRPQLAEHNIHVLRYEELSKSQRNKLRVYFER
                     EIFPVLTPFASDPSRPFPHISNLSLNLAVQVRDPKTGDLRFARVKVPPPLSRLVPLKR
                     FDPDQLSTPGVQKFVWSEEVIAANLDRLFPGMEIVAAYPFRVTRNSDMELQEEEADDL
                     LLMMEDNLRQRHFGTVVRLEIDERMSPEIRAILEANLGVDSYDIYTTNGPLGLSSLWE
                     LHRLERPDLKDKPFTPQAPPALQNIRGSIFSVLRQRDVLLHHPYESFSPVINLLEAAA
                     DDPDVLAIKMTLYRVGSTPPVLQALKKARLNNKQVAAVVELKARFDEETNIHWAQELE
                     SAGVHVAYGLIGLKTHCKVTLIVRRERDGIRRYVHLSSGNYNSTTARTYTDIGFMSCD
                     EALADDASDLFNFLTGYSRQEEFRKFLVAPMTIRPKLMEFIQRETQLGPKGRIIFKCN
                     SLVDGEIIRNLYKASQAGVKIDLIIRGICGLRPGIPGVSENIQVMSIVGRFLEHSRIY
                     YFHNDGAPSLYMGSADLMPRNLDRRVEVLFPIEDADMQTHIVEDILAVCLRDTAKSHI
                     LQSDGRYIPRRYLLDGDGPLFDSQAWFLMQSGGKVAEYL"
     gene            11374..>12498
                     /locus_tag="PLC06_05570"
     CDS             11374..>12498
                     /locus_tag="PLC06_05570"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRO89948.1"
                     /translation="MEVVAVPPVDVQPPPTPPITSDPSATLPTPADASEGLLPTPTLA
                     GAADSVEQTNPTAATEGAAQQPPPPPTATADPAIVAQYTLSAAPGVPEDLIVAAQTLV
                     ANNRQLFQWVEDDATADVRLTVNDGTPLANWIYVVAAPFATVADDTTWPAVTSGWTTG
                     ESELGRLHVDPATSAALTAVWGPGGAQVVAEDFIGVVWAARPSWIIIPFEHLRPEWKV
                     IGLDGRSPLTHDFSPDGYPLSLPMTVTGQPQAVNEFVARWNGPATNRDPAKLTRVAMS
                     GVTALVRATAAQMEINGILWPGEEVAPVFQSADIAHISNEVSFAADCPYPDAYGGTTF
                     CSRDPYFDLIKHLGIDVIELTGNHLNDYGREPLSRTIDMYE"
BASE COUNT         2477 a         3875 c         3740 g         2406 t
ORIGIN      
        1 tccagcacca gccgccgcga caagggaaaa ggacgaacag caaaaccctc gtcatgctct
       61 tttttcatgg ggccagtatg acataccgcg ccgccccggt tcaatgacaa acggccgaaa
      121 aattggcgcg aattgtcatg atcaactgct gtagaaaccg agtttctcgg aagaaactcg
      181 gtttctaagc gcagctcact ggaaaatctg tgaaattggt gtaatctgtg gttccttctt
      241 ctcttatcag gagatatcaa ctatgtcaca atacgctcac ggtggcgaac tcgtcgcccg
      301 ttcgctggcg gcccagggcg tccgcaccat ctacaccctc tgcggcggcc acgtcgcgcc
      361 catctacgac ggctgcctga accacgacat cgccgtcatc gactttcggc acgagcaggc
      421 ggccgcccac gccgccgacg cccacgcccg gctgacgcgc aatataggcg tggccctcgt
      481 caccgccggg ccgggcgtca ccgatgccgt caccggtgtc gccaacgcct ggcaggcccg
      541 cagtccaatg atcctgctcg gcggggccgc gccactcaag acaaagggca tgggcgcgct
      601 gcaggaaatg ccgcaagtgg agatgttccg caccttcacc aaggccgcct tcactatcga
      661 gcgcaccgag gacatccccg aacgaatggc cgaggcctat cacgccgccc tcagcggccg
      721 ccccggcccg gtcttcattg aactgccgtt cgacgtgctg ttcaatgccg tcgattcgcc
      781 ggagcgaatg cccgaccggg tgaccatcga ccccgtcgca ccgccggtcg acgccgtgca
      841 ggccatgttc gacctgctgc gcgccgccga gcggccgctc atcatcgccg ggacgcaggt
      901 ctactgggac ggcgcgagcg acgccctgcg cgagctgacc gaccagacgg ccatccccgt
      961 cttcaccaac ggtgcggggc gcggcacgtt gtcgatggac cacccccact gcttcaaggc
     1021 cacgcgcggc aaggcgctgc gggcggccga cgtcgtcctg ctcatcggca cgccactcga
     1081 cttccgcctc aagtacggcc gcgaggactg gaatcccgac agcaagctga tccagatcga
     1141 gaacgacgcc gccgaactga accacaaccg gacagccgat gtggccctga cggccgacgc
     1201 ccggctggtg ctggaggcgc tggccgaggg gctgcaaggc atccgcttcc agccgtggct
     1261 ggatcaggtg gccgagtggg agcgggcgcg caacgagaag caggcggcgt gggaagcgct
     1321 ggaagacgcg ccggtgaatc atttccgctt cgcggccgag gtcaatcgct tcgtggacgg
     1381 gaacactatc gtcatcggcg acggcggcga tatcgtcagc gcctgtgcca aggtgctcaa
     1441 ggttactcaa ccggggcaat ggctcgatcc cggcccactg ggctgcctgg gtgtcggcgc
     1501 gccgttcgcc gtcgccgcgc aacacctcta ccccgaccgg cgcgtgctca tcatcagcgg
     1561 cgacggctcg ttcgggctga acggcttcga attcgacacg gccgcgcgct ttgggctgcc
     1621 catcgtcgcc atcgtcggca acgacgccgg gtgggggcag attcgcgggc cgcaggtgca
     1681 gatggtcggc gcggagcggg ccatcgccac ctcgctggct cccacgcgct acgacaaggt
     1741 ggtcgaggcg ctgggcggct acggcgaact ggtcgaggat ccggatggcg ttctccccgc
     1801 gctggaacgg gctttcgctt cagggttgcc ggcttgcatc aacgtgatgc tcgacccggc
     1861 gggcatgagc aagacgggag cgagttcgcc gtatattgtg tgatggattg gatggaatcc
     1921 gaatcaccag acaattggta caagtcgcgc gtttgtgaga tcctcatccc gcgtaacaag
     1981 gctgacatcc aagcccaagg attggatata gagcgctgtt gccactatca gcctatcgtg
     2041 caattctgtt attcgggaag cctctgaagt gaggctgagc ttgaagatat ccagcgtaag
     2101 tggatggatt tcaaagcgat ggtctttggc aagagaatcg atcaagtagg caacggaagg
     2161 aatcttcgtt cgaccatggc cgataatgat tcccgcctca gccaggacga ttatcggcac
     2221 aaccaaatga ctctcgactg ccgatatctt ggcttgggca cgtgatccga gacgtcgatt
     2281 cccttccaaa taccagagca gtgaatgagt atcaaggacg aaactactca ccgcttaatt
     2341 cgcgctcgga tggccgccat tcggcaatga cgtaatcttc aatggtcgag aacgggccat
     2401 cgaagtcacg gaattctcca aattggagag tttccgtctt tttgagggga gagggaatca
     2461 gcgtttcgag aataccatgc atcaatcgca cccgatattc gggtggcaat aaactgattc
     2521 gttccagcaa ggcttccaaa tcccgattgt cgatgatcat cgttcaactt cccgaaaagt
     2581 ttctatccag ccaaatctta ccatagcgga ctacctgttt caaacaacac gccgcctttt
     2641 aaacagcaac tcttacataa tcccctcaaa aaacgccggc cgctgcgcca gataccggtt
     2701 cacaatcgcc acggcctctt ccactccccg ctcggcctga atccgccgcc ccatctcggc
     2761 cgcccgcgag cgcattgcct catcgcttgt cgccgtctca atggccgcgg caagacgccc
     2821 ggccgtcaac tcgctctgtg aaatggggcg cggccccgca ccaagttcat atacccggtc
     2881 gccccagaac ggctggtcgc cgaagaaggg cacgacgacg ttgggcacgc ccgcccgcag
     2941 tcccacgtgg gttgtgccga cgccgccgtg atggacgacg gccgccatgt gcggaaagag
     3001 ccagtcgtgg ggcacggcgt cgagtcgcag caccgaatcg ggcaggtcgc cctcggccag
     3061 cccggcccac ccactgagca gtaccccgcg ccgcccgctc agccgcagcg cctccagcgt
     3121 gacggccgtc agccgttccg ggtcgttgcc ggtcatgctg ccgaagccga cgtaaaccgg
     3181 cggctcgccc gaagccaaaa atgtcgccag ggcctcagat ggcgtgtaag ccggatcgtc
     3241 ctggaaccag aagccggtga cgaatgctga ctcgttccag tcgtgtggct tgggcagcac
     3301 cgtcggacta tagccgtgga cgaccggcaa gcgccgccgc aacatgtcca gaaatggccc
     3361 gccgaaggaa aacggccgca acttcaacag atcgcgccgg acgtcgttca tgatcggccg
     3421 gatcatctgc cagaacagtt gtccgccgat gacgtgggtc gcgtagttga acagcgcgcc
     3481 gcccttgtgt cgggtaggga agatagcgct ggggaactcg cgggtcgggt tgaccggttg
     3541 caaatagctt tgaatgaacg gcttgcccag cttttcggcg acgttataag ccgtataaaa
     3601 agcggggccg gagaacagga tcagatccgt gccgtcgcag gccgccagca cgtcggctgt
     3661 cgcttgatgg aggatggggc cgatcagctc gcgaaacccg cgcacaaacc ccaggccccg
     3721 gcggccggtc tccatccagt cgcgtccttg ccgcccctgc actgcctcca ttggatcgcc
     3781 ttcgacgggg ctgaaaccca acccattact ctgaatccag tcggcataga gggtatgtgt
     3841 cgccaatctg accgtgtggc cattcgccgt taggcctagc cccagcgcga tgaacggctg
     3901 gacgtcgccg cgagaaccgg tggtgacaat cgtgatgtga gccatgaacc gggttcgcct
     3961 ccgatagtgt ggtaaaattt gcactagtcc gtccattcga cggacacacc ggatactatc
     4021 aaagcgcgac cccgggtcaa gcgcgaagga tttgaatgga tttcaaggat aatgtagtga
     4081 ttctgaccgg cgcgtcaacg ggaattggtg aggcgttggc tcatgcgttg gcgaagcagg
     4141 gagcatggtt ggtgctggcg gcgcgcaacg cccagaaact ggaggcggtg gcggccgagt
     4201 gtcgcgcgcg cggcggccgc gccctcgtcg tggccaccga cgtgaccgac gaggatgagt
     4261 gtcgcgaatt gatcgaacgc gccgcggccg agtatggccg gatcgacacg cttatcaaca
     4321 acgccggtct cagcatgtgg atgaagctgg aagacgttga ggatctgtcg acgctggagt
     4381 atctcatgcg cgtcaactac ttcggcagca tgtattgcac ctactacgct ttgccgtggt
     4441 tgaagcagtc aaaggggcgc attgtggcca ttgccagcgt tgccgcgaga acgggtgtgc
     4501 ccacccgtac cggctactcc gccagcaaac acgccatggt gggcttcttt gaatcgttgc
     4561 gcattgaggt tgaggatgac ggcgtcagcg tgactatcgc ctatcctgac ttcgttgcgt
     4621 cgggaatgca cactcgctcg ctcggcgcgg acggcaagcc attaggccac aatccgcttc
     4681 aactggacaa gatcatgacc agcgaaacgt gcgcccggct gatcatcgag ggagcagccg
     4741 cgcgcaagcg agagatcgtc atgtccaacc gcgcgcgctt cggccagtgg cttaagctga
     4801 ttgcgcccgg tagggtggac aggattgcaa agaaggcgat tgagaagggg cgttagggag
     4861 caggggagaa ggggagaaag ggagaaaggg tgctcccttt cccgcttcct cccttttccc
     4921 cctgctcaac taggaggcat tcgccgtaaa gtcgatatac agttcgacat cttcctcgac
     4981 attggccacg ttgggcacgc tggggatttg caggtcgtaa tcgacgcggt tgacgatggc
     5041 cgtggccgag ccggtgatct gggtatccga aacggccgtg gcctcaaccg tgaaggtcgc
     5101 cggcaaggtg atgtcgcgga tcgtcagatc gcctgtcaat gtgaaagtca ccgtatcgcc
     5161 gacggcagca gtggccggca gcccttccac cccggtgggt gtgaaggtga tgaattcgaa
     5221 ctgaccggta tccagaattt cgttctggat ggcccgattg cggaagttgt tgtccgtcag
     5281 taacgtgcga gcattgatct gaatgatccc cacctcggtc tgactcaggt cggccagatt
     5341 gagcgacaat tggcccgcga cctgatcggt cgtgccgatg accgtcttgc gctcgccgcg
     5401 caggtcttcg tctaactgga atcgtacctc ggacgcggcc gaatcaatgg tgaaaactct
     5461 tgctccggct gcgggcgctt cggcaggggc ttcggccgtt ggttcatcgg ccgtggtttc
     5521 ggtggaggcg ggatcggccg aagtctcggt tgcctcgacg ggcgcaacgc ccgccgactg
     5581 cgtctccagc gggattgcct cgatcggccc gctggccgcc tcaggctcct gcaacacgcc
     5641 gcaacccacc agggcgaatg cagcgatcag tgacaacaat agatatacgg ttaatttctt
     5701 catccatctc cttggctgtt ggtttccggg caaatcgccc gcgaattaac gcatgactct
     5761 gccatctcga gatgagaaca ggctgaatca agtatagcga acacattaat attcctcatg
     5821 ggttatatgc catgggccga cattggcagt gcgatcgtga attgtgcccc accggccaca
     5881 ttcccggccg agatgctgcc gccatgcgcg ccgatcaggg atcgggcgat ggccaaccct
     5941 agtcctgatg aaccatccga tgttcgtgcc atgtcgccgc gatagaagcg atcgaagatc
     6001 tggggcagct cctcggccgg gatgcctgag ccggtatcgc gcacgcgcaa caagacctga
     6061 tcgtcgtgct gtgtggcgga cagggaaatg cttcctccat ctggcgtatg gcgcagggcg
     6121 ttgctgacca ggttccccaa cacctgcagg atgcggtcgg ggtcaaccag gatcggcgga
     6181 acgtcggcgg cttccacgag cagggcgatt tgccgagctt cggccgccgg tagataggcc
     6241 aacgctgtgt gctccagcaa accgcgcggc tccaccgggc gacgcacgag cgaaagctgc
     6301 ccggcatcgg ccagcgagag ggtgcgcaaa tcctcgatga gccggttgag atgttgcgcc
     6361 tgccggtgaa ttgcttcgta ggtttccggg ccgccgggca gtttgccgtc ggccaatgcc
     6421 tcggaataac ccaggatgac gctgagcggc gtgcgcaagt catgggcgat atcggccgtc
     6481 atttggcggc gcaactggtt cgagtgagcc aggtcggcgc tcatctggtt gaatgaatgg
     6541 gctagctcgc cgatttcgtc tgaggtgcgc accggcacct ggtgccccag gtcgccgccg
     6601 gccagcacct tggtcgcctc agtcagctcc cgcaccggcc gggagatcgt gccggccagg
     6661 ataatcccca gcaacaaggc cagcacggcc gctccggccg cgctccaccg aacagcccga
     6721 tccacggccg tcagaaacct ggcttccggc gagccgcgaa cgtctcgcgc gctgcttcga
     6781 tccgcaaacc gcaaataacc gactacgtcg ccatccactt ctaccggcaa ctgatcgccg
     6841 tcatcgccaa ttgcgcttct ccggccaatg gcatcgccgg ggatcgtgct gaagacgacg
     6901 agaccatcgc tgtccgtcag ggatatgagg ggtatcatcc cgccattcac agcggcgcct
     6961 ccggggccac gtcgccaccg caacgctccg cgcaagttgt cccaactgcc gttcctggcg
     7021 tagtactcgg tcacctccgc caatacgtca tcgtgatagc ggtcgaccac gaatcgatcg
     7081 aactctcgac gggtcatgcg gccgacaaac acagagacca ggacgactcc ggtcaggccg
     7141 acaatcagaa aagcgagggt gagcttcagg gataatgaac gcatgggatt acgccggaat
     7201 tggctcggcc gccagccgat agccgatgcc atacaccgtc acaatgtagc gagggttccg
     7261 gggatcagac tcgatcttgg ctcgcaaatt gcggatatgc acgtcaatag tgcgttcgta
     7321 gccttcgtag gcgtcgcctt gaagcttgtc aagaagctcc agacgggaaa acacgcgccc
     7381 cggtgaagac agaaatgttg ccagcaagtc gaactccgac ggagtcaggt caaccgaagc
     7441 ctggcccacg gtgacctcgc gtcgatcccg atcaagtgtc aggtcacccg cacgcaaaag
     7501 cgccgatggg ccgaccccac cgcctttgtc gagacgtcgc aggacggccc gaacccgagc
     7561 agccagttcg cgcatgctga acggcttggt caggtaatca tcggccccca gttccagccc
     7621 caggaccttg tcgctttcct cgatccgggc cgtcaggatg atgattggcg catcgccctc
     7681 gcgagcatac gctttgatga agtcaaagcc tcccaattcg ggcatcatca gatcaaggat
     7741 gatcaaatca ggacgctcct ggcgcgccac aaacaggccc tcgcgaccat tggcggccgt
     7801 gaccacgcga aagccttcct gtaccaggta gctcctgacc agcattcgca ggctctccgc
     7861 gtcatcgatg accagaatcg tcttgctcat aaccgcgctc accattcact cggatcgatg
     7921 gagctcaata tcaggttggg aggaattata cactgcttcc ttcctccacc gtttaacgtc
     7981 tgtcggatac agatcggcgc ctgcatgtgc agcggtcgtc cgtcatccgt cgtccgtcgt
     8041 cggtcgcccg aacagacaca ggaagggcag acccacaggc ctgcccttcc acacgatcag
     8101 tattcaacct ctagcgtatc aggcatcgaa acccgtgtcg gtcgtttctg ccccgccttc
     8161 attgggggat gactggccgc caaagccgtg gaaaccaccc cggccgccgc ggccgccacc
     8221 acgatgaccg ccgccgaaac caccgcggcc gaaaaggtta agcccattcc cggccaggat
     8281 ttcgtcggcc tgagcctggg tgatcacgcc atcggtcacc gcctgttgca cggccgcttc
     8341 gtaggccgcc ttggcattgg cttgcagagt agtcgagtcg atgccttgct cgaccataag
     8401 atcgactatc gacttgccgt cggcaagcgc cgctttcagg tcgtcgacag acatgcccag
     8461 cgccgtagcc agaatggcct ggcgatcgat gtaactcttc aacgcgtgtt gagcgagaat
     8521 ttgatccgcc tgttcctggg ttatctggcc ggcagtaacg gcatcagcca ccgaagcggc
     8581 gaaggctcgc tcctgggcat cctccaactc gccgacggtg atgcccagcg cctcggccag
     8641 attctccagc cagttggtgt tgtccccgaa gcgactaccg aagccgcccc gatgtccgaa
     8701 cccgaaccac cccttggtct cgggggttac ctcgctttcc tgagccggta cttccgcctc
     8761 cggcgtgctt ccttgcgcca gggcaatgct gccgagcaag cccagcatca acgccagcac
     8821 accggcgatt accaacagag accatttctt tgatctatcc atttcatatc tccttgttgc
     8881 tccgagtcgg ttcgggattg acccggctcg gtattcaact tatatctgct atcctaccgg
     8941 cgaactgttc agaacttgtt aagaactgtt tgggatttta tgagaatgaa acgggggtac
     9001 gcggcagaac tcgacatctg ccggatgacg gacgtctgcc gcaaggggac aggaggagac
     9061 ccagagagag tcgatgcgct tctacaggta ttcggcgacc ttgccgccgc tctgcatcag
     9121 gaaccaggcc tggctatcga acagcggccc atccccatcg agcaggtagc ggcgcggtat
     9181 gtatcgacca tcgctttgca ggatatggct cttggccgta tcgcgcaggc aaacagccag
     9241 gatatcctcg acgatatggg tctgcatatc ggcatcttca atggggaaga gcacctcaac
     9301 ccgacggtca agattgcgcg gcattagatc ggcgctgccc atgtaaaggc tcggcgcgcc
     9361 atcgttgtgg aaataataaa tgcgcgaatg ctccaggaag cggccgacga tgctcatcac
     9421 ctgaatattc tcgctcacgc cgggaatgcc cgggcgcagt ccgcagatgc cgcggataat
     9481 gagatcgatt ttcacccccg cctgcgacgc cttgtagaga ttgcggatta tctcgccatc
     9541 gaccagagaa ttacatttga agatgattcg tcctttcggg ccgagttgcg tttcgcgctg
     9601 gatgaattcc atcaacttcg gccgaatcgt catgggagcg acgaggaatt tacggaactc
     9661 ttcctgtcga gaatagccgg tcaggaaatt gaacagatcg ctggcgtcgt cagccagcgc
     9721 ttcgtcgcag ctcatgaagc cgatatcggt ataggttcgc gctgtggtgc tattgtaatt
     9781 gccgctggag aggtggacgt aacggcgaat cccgtcgcgt tcgcgccgca caatcagcgt
     9841 gaccttgcaa tgcgtcttca gaccaatcag cccgtaggct acatggacgc ccgcgctctc
     9901 cagctcctgc gcccagtgga tgttggtttc ctcatcgaat cgggctttta gttccaccac
     9961 ggccgctacc tgcttgttgt tgaggcgggc cttcttcaag gcttgcagaa cgggtggcgt
    10021 ggagcctacg cgatagaggg tcatcttgat ggccaacacg tcggggtcgt cggccgcggc
    10081 ctccagtaaa ttgatcaccg gcgaaaagct ttcatacgga tgatggagca ggacatcgcg
    10141 ctgccgcaac acgctgaaga tgctgccgcg gatgttttgt agtgccggcg gtgcttgcgg
    10201 agtgaagggc ttgtccttca ggtccggtct ctcaaggcgg tgtaactccc acaagctgct
    10261 cagtccaagc ggcccgttgg tggtgtagat gtcataggaa tctacgccca ggttcgcctc
    10321 caggatggcg cggatttccg gcgacattcg ctcatcaatc tccagtcgca cgaccgtgcc
    10381 gaaatggcgc tggcgcaggt tgtcttccat catgagcagc aagtcgtcgg cctcttcttc
    10441 ctgcagttcc atgtcgctgt tgcgcgtcac gcggaacggg taggcggcga cgatctccat
    10501 gccggggaac aatcgatcga gattggcggc gatgacttct tcggaccaga cgaatttctg
    10561 aacgcctggc gttgagagtt gatcggggtc gaagcgtttc aacggcacca gccgagataa
    10621 gggtggtggc accttcacac gggcgaagcg aaggtcgccc gtcttgggat cacgcacctg
    10681 caccgccagg ttgagactga ggttggagat atgagggaac ggccggctgg gatccgatgc
    10741 gaacggtgtg agcaccggga atatttcccg ctcgaagtag acgcgcagct tgttgcgctg
    10801 cgatttgctc aactcctcgt agcgcaggac gtggatattg tgctccgcca attgggggcg
    10861 aagatggtcg cgccagatgg agttcgcttc ggagaacagt tgcgtcagag tgcgatggat
    10921 ggttgccaat tgttcgcgcg gcagcaggcc gtcggacgag acggtcgtga ggcccagctt
    10981 gaattgctgc ttgaggcccg acacgcgcac catgaagaac tcgtccatat tgctgtggaa
    11041 gatggccagg aacttgaccc gctccagcag aggatgattg gactgcgtct cgtgcagcac
    11101 gcgccgattg aattcaatgc tgctcagctc gcgattgata tagaggctgg gatccctcaa
    11161 atccacgagc ggcggcttcg cggctacctt ggcgttgccg ggttttcgcg tctcttgaac
    11221 aggttccggc ttcgtaatct cgaccatttc gcctccggga cgattaccat tagcacggca
    11281 gtgggaattg cgttactctt tcacataatg cgtaagttcg tattatacat tgttcttctc
    11341 actctggtaa tcgggggctg cgggggaaag ggagtggagg tagtcgccgt acctcctgtg
    11401 gacgttcagc cgccacccac gccgccgatc acctctgacc caagcgcaac gcttcccaca
    11461 ccagccgacg cgagcgaggg cctgttgccc acgcctacgc tggccggcgc ggccgattcc
    11521 gtcgagcaaa ctaacccaac cgccgccacc gaaggagccg ctcaacaacc gccgccaccg
    11581 ccgaccgcca ccgccgatcc ggccatcgtc gcacagtaca cactctcggc cgcgccgggc
    11641 gttcctgaag atctcatcgt cgccgcgcaa acgctcgtcg ccaacaaccg gcagctcttc
    11701 caatgggtcg aggacgatgc cacggccgac gtccgactga ccgtcaacga cggcactccg
    11761 ctggccaatt ggatctacgt cgtcgccgcg ccgtttgcta ccgtcgccga tgatacgacc
    11821 tggcccgcgg tgactagcgg ctggacgacc ggcgagtccg aactcggccg cctccacgtc
    11881 gatccggcga cgtccgccgc gctgactgcg gtatgggggc cgggcggagc gcaagtggtc
    11941 gccgaagatt tcatcggtgt ggtgtgggcg gcccgcccgt catggatcat catcccgttc
    12001 gagcatttgc ggccggaatg gaaagtcatc ggtctcgacg gccgctcgcc gctgacccat
    12061 gacttcagtc ctgacggtta cccgctttcc ctgccgatga ccgtcaccgg acaaccgcag
    12121 gcggtcaatg aattcgtcgc ccgctggaat ggcccggcca cgaatcgcga cccggccaag
    12181 ctgacccgcg tcgccatgag cggcgtcacc gcactcgtcc gcgccacggc ggcacagatg
    12241 gagatcaatg gcatcctctg gccgggcgaa gaggttgcgc cggtgttcca atcggccgat
    12301 atcgcccaca tttccaatga agtctccttc gccgccgatt gcccctatcc cgacgcctat
    12361 ggcgggacga ctttctgctc gcgggatccg tatttcgacc tgatcaagca tctgggcatc
    12421 gatgtcatcg aattgaccgg caatcacctc aacgactatg gccgcgagcc gctgtcgcgg
    12481 actattgaca tgtatgag
//
LOCUS       DAOMQG010000065        12158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2258_length_12158_cov_11.172437, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000065 DAOMQG010000000
VERSION     DAOMQG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_12158_cov_11.172437"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..1772)
                     /locus_tag="PLC06_05575"
     CDS             complement(351..1772)
                     /locus_tag="PLC06_05575"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRO89949.1"
                     /translation="MTTQRVRTIFTVWLMILDFLMIALAFVLAYQLRVSIDWPEPLAN
                     QVPLADYFSLFLAQAFGIIVLLFLNRQYYIPRAASRIDQVYYVFISVSVGILIAVALS
                     TIFFRNDRAILDYPRTMLVYNWLLTMILLMLGRFLTNWLRRQLQERGIGKDRVLLVGT
                     GDTARMILQRIVWSPGLGYDLVGIINGDTPETEIMGMPILGKPEDLPDLISEHRIDEV
                     IVAIPEKGHRETLHVISYCERGRVTIKVFPDLFQYITTTAGIEDLGGLPLLSVRDYAL
                     RGYLLTMKRLIDLLGSAVGLVVLSPLMFLTAVAIKLESPGPVFFVQPRMGLDGKEFML
                     IKFRSMRTDAEKDGPGWTVKDDPRQTRLGTWLRRLEMDELPNLINVFLGEMSLVGPRP
                     EQAYYVEQFRRYVPRYMDRHREKGGMTGWAQVNGLRGDSSISERTKYDLWYSENWSIL
                     LDTKILLRTLWQIVSPRGEEPRY"
     gene            complement(1784..3550)
                     /gene="recN"
                     /locus_tag="PLC06_05580"
     CDS             complement(1784..3550)
                     /gene="recN"
                     /locus_tag="PLC06_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRO89950.1"
                     /translation="MLSELFVRNFAIIDELRLQLDPGFNVLTGETGAGKSIILDAVTL
                     ILGGRADSTVIRAGKDEAYVEATFQLDATLRETLRPVLETEGLDEEGVDYVVLAREMR
                     ANGRNICRVNGRTVSLSVLRQVADPLIDIHGQGEHLSLLRPRAHLPLLDAFGGLESER
                     RELAAEVHKLHAIQRELEALRRSSRDMNQRIEMLQFKVQEIIAAALKPDDEESLRVER
                     IRLANAEQLGRFAGEAIALLSTVDEDRSSVIDLLGRVEHDMAQLARLDDTRQESLSNL
                     QGIGFQLNEIAAELQDYLDALEYDPRRLNAVEGRLELISDLKRKYGEASVAGLLAAAE
                     KAEAELRTLENSGTRTAELEAEQETFLRRIGRMAGALSQKRRAAAEQLSAAVETHLAD
                     LKMEGARFGVDFQAQDTPAGAILSLDGEERRLAFDQTGIDQVEFLISANPGEPLKPMA
                     RVASGGETARLMLALKTALAQVDQTPTLIFDEIDQGIGGRVGDVVGRKLWGLAAEAGH
                     QVVVVTHLPQLAGYGDIHFHVSKAVADGRTITHVDALDMVGRVRELAAMLGTQGDTAV
                     SGAESILRHVNQAKNGLPIEEY"
     gene            complement(3563..4447)
                     /locus_tag="PLC06_05585"
     CDS             complement(3563..4447)
                     /locus_tag="PLC06_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89951.1"
                     /translation="MAIKEPRLRSLLTQAERNATYGKNAAAETIYRQILGEAPDAEEA
                     WLGLAEVVHDPAEKRAAYERALALAPDLPAAIAGLAILDGRPIPPEVEAALAAANAEE
                     EADDPLPATIVENQSEESHDAEVYELACYRHPDRPTSLRCYNCNRPICISCAIKTPVG
                     YICPECRRVAEDAFFNNKPLDYLIAVLVSLPISLLVGYLMVNFFRGGFLFFMLIFFLS
                     GAVGGFIGRITKRAIGGRRGRYLPGLVVAMMILGVAIPALPLLLAVLTGNMGGLFALL
                     GPGIYLFVGASAAYWQMR"
     gene            complement(4557..5426)
                     /locus_tag="PLC06_05590"
     CDS             complement(4557..5426)
                     /locus_tag="PLC06_05590"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRO89952.1"
                     /translation="MNLIGILEQPRIPESAPLSKEVRDWLSARGVPSWTAAPMDDEDL
                     NAPLEGTSLLIVLGGDGSTLRAARWSVPYGIPLFVINVGRVGFLSEATPENWPEKLER
                     LLRGEYWIERRLLLHAELWRGNECLMPFSALNEVVIGRGAQARVIHLHLHVDGDLVTT
                     YIADALIIATPTGSTAYAMAAGGPLMPPQLQNLVVVPVAAHLSLNRSLVLHENARISI
                     RVEMSHEAYLTADGQKSTPVQDGDEIVITKNEQVCHFARIESAGYFYRRLMGRLGFSW
                     PHLNIGLSRDEKP"
     gene            complement(5479..6762)
                     /gene="hisS"
                     /locus_tag="PLC06_05595"
     CDS             complement(5479..6762)
                     /gene="hisS"
                     /locus_tag="PLC06_05595"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRO89953.1"
                     /translation="MSNQIQRLTGMHDVLPDDRRYWDVIIAAAGSLTQRYGFQHIDTP
                     ILEATELFVRGVGQGSDFFVQKEMYTIQEADDSSITLRPEFTAGFVRAYISNGMHSWP
                     QPVKLYTIGPSFRRERPQAGRLRQHSQINCEILGEEDPAADVEVMMLAMNLYRELGFR
                     GLTFQLNSTGCPVCKPGYIEKLVAYLGDHHDRLAAIDKERLTKNPLRVLDSKEPGMDE
                     LLAGAPHIADHLCDDCREHFADVRELLDALNQSYTINFRLVRGIDYYTKTVFEVWQEG
                     IGAQSALCGGGRYDGLAEDIGGPSTPGVGFGSGIERIVLGMREQKVDLPEVLPPPVLV
                     AHFGGTTKISAVGMAFRLREAGIGAHLAFARRGRSMKSQMREADRHGARYVLIMGENE
                     LVQGVVAVRPLVEGEEQAVVPLSEIVTWLRERLAA"
     gene            complement(6878..7423)
                     /locus_tag="PLC06_05600"
     CDS             complement(6878..7423)
                     /locus_tag="PLC06_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HRO89954.1"
                     /translation="MSETMTMSGLPLILVLFLLFLAAILWFRGRDMRKESGLPPGNVI
                     YTDTGAWRANNNVFHASEYRLVGKPDYLVEQRDGSIIPVEVKSGPAPDSPWDGQVLQL
                     AAYCLLVEVNYGVRPPYGILQYRDRAVAVDYTDELEADLLDLLDEMRATREDFDPEPD
                     HADRRRCAACGVRDACDRRLA"
     gene            complement(7451..7690)
                     /locus_tag="PLC06_05605"
     CDS             complement(7451..7690)
                     /locus_tag="PLC06_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89955.1"
                     /translation="MSYSWIRASEIGEYVYCRRSWWLRRVRAVASTNVAQMQAGTQYH
                     QRHGRLVEQSVWLRRLAYVALFCAVAMLVFELLSR"
     gene            complement(7739..8662)
                     /locus_tag="PLC06_05610"
     CDS             complement(7739..8662)
                     /locus_tag="PLC06_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRO89956.1"
                     /translation="MGNVLNQLQKSPLGLISQVRTGFLPAIKLWGGLTLRSIQDALAP
                     ESQLVASSIGYFTLFSVFPLALLVVAIASNWLDPMLAEARIVAELEFIIPGLETMLGE
                     NLQNLIRARGPITGVAAIMLIWSASSVFNVVTRAMDRIWGADINHRRSVWRHRTLAVG
                     MVLIITMLLLLASSIEGTVLTIINSLVPSTLMDIGPFTTSFWAVFLNVLLFSLLYYFI
                     PHVKIGWREVIPGAIMAGLMWHFAKQLFLYFVGTYLSRSNLIYGSVGTIIAFLTWTYI
                     SSLVLLCGAYLNRYAAAYRQLGPSATFRAIE"
     gene            8805..>9571
                     /locus_tag="PLC06_05615"
     CDS             8805..>9571
                     /locus_tag="PLC06_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRO89957.1"
                     /translation="MKRPESLREYRWLVAPEPPPGSPLHTIGDTIHPVLRQVLYNRGL
                     TQPGQIQSFLENHYLQSRDPFGLPDMEPAVERITRALRDGEMIAVYGDFDADGVTATV
                     LLTEALRSMVADRRQIIPYIPDRFDEGYGLNLDSLAKLRAKGVSLVISVDCGIRSPVE
                     AAHARSIGLDLIITDHHGLGREIPPAVAVINPKRPDSTYPEQMLAGVGIAFKLAQAMR
                     QFAPDLVTGDESNLLDLVAIGTVADLAPLLGENRKLVA"
     assembly_gap    9572..9671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9672..10421
                     /locus_tag="PLC06_05620"
     CDS             <9672..10421
                     /locus_tag="PLC06_05620"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRO89958.1"
                     /translation="ALAEGMVDPEAPIIIARNTEFNSGIVGLVAGRLAEKNYRPAIIM
                     EEGEEESRGSCRSIPEFHITKALDEVADLLVRHGGHAQAAGFTIRNEHVPEFISRMTR
                     IAGEWLDGHDLRPSLAIDADLPLSDIDWALQGTIEQLEPTGFENPKPLLLSRAVEVVS
                     HRPVGTDGSHLQLHVVDARRNGADGHSGAKRQPARPYPAIAFRQGEWAAHMPRYADIV
                     YRLGVNHWQGSANLQLVIEDIRPTEEESIAV"
     gene            10418..11380
                     /locus_tag="PLC06_05625"
     CDS             10418..11380
                     /locus_tag="PLC06_05625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO89959.1"
                     /translation="MTILVTGAAGFIGSHLAERLCRRGDRVIGLDNFNDYYDPTRKRA
                     NEQRLNTYPNFRMVEADLRDRTALFDLFATEKIDAVAHLAAMAGVRDAIKFPNLYVNV
                     DLNGTQNVLDAVHAVGGVANFVFASTSSVYGNTTQVPFVETDPCDRPLQPYAAAKRAA
                     EILGYTYHYLFGQNFTVLRFFTVYGPNGRPDMMAYLLADSLMKGREIPLYDNGQMYRD
                     WTFVDDTVSGIVAAIDRPLGYEIINLGRGEPVLLRDFVEIMEELSGRKANVVPTPRLA
                     ADFVRNEADISKARRLLDYDPQVSVADGVERFWKWYQRYDAGQG"
     gene            11361..>12158
                     /locus_tag="PLC06_05630"
     CDS             11361..>12158
                     /locus_tag="PLC06_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRO89960.1"
                     /translation="MMPGKDNAPPALGPFHYVANEAKWTACLRDLQAAPRLAIDLEAN
                     SMFAYRERVCLIQVSTADTDYIIDPIQHLDLSGLGAIIADPRVEKVFHASEYDLILMR
                     RDYDWHLDNLFDTMWAVRILGYSQMGLASLLENFFGVSTSKRFQKANWCKRPLSSAEL
                     AYAQTDTHYLLPLRDRLAAELKAVDRWEEATEIFREQAQVRLPNNNFDPDGFWFINGA
                     FDLPPEKQAALRGLYLFRDREAKRRDVPPFKILGERTLVELAEKLPGR"
BASE COUNT         2603 a         3745 c         3451 g         2259 t
ORIGIN      
        1 gtggcggaac taaaatccag cccatattcc tcgacaagcg ccttcgagcc gaccggtttg
       61 cccgtctcaa tgtaactacg gacaaccatc gccagaattc gctcttgccg ttcggttagc
      121 tgctgaaatt caaccatggc cacaatgcaa tcatcgaccg ggtgactagc tcgcaggaca
      181 agctataccc ggtcgcgcct tactctcatt cgctttaatc cttgcttagc actcaaacat
      241 attgagtgct aacaggagcc ttcaaggatc ataacatgcc gcctaatcag cgtcaataag
      301 aaggatatta gattatgagt gttagaaagc tgatagaact aatagtagaa ctaatagcga
      361 ggttcttcac cgcgtggcga gacgatctgc cagagagtgc gcagcagaat cttggtatcg
      421 agcaggatcg accagttctc tgaataccac aaatcgtact tggtgcgttc gctgatcgag
      481 ctgtcgccgc gcaaaccgtt cacctgcgcc caaccggtca tgccgccctt ctcgcgatgg
      541 cgatccatat agcggggcac gtagcgccga aactgctcga cgtagtaagc ctgttccggt
      601 cgcggcccca ccaggctcat ctcgcccaga aagacgttga tcaggttcgg caattcgtcc
      661 atttcgaggc gacgcagcca ggtgcccagc cgtgtctggc gcggatcatc cttcaccgtc
      721 cagccggggc cgtccttttc ggcgtcggtg cgcatggaac gaaacttgat caacatgaat
      781 tccttgccgt caaggcccat gcgcggctgg acaaagaaga ccggcccagg cgactccagc
      841 ttgatagcta cggccgtcag gaacatcagc ggcgagagca caaccagccc gacggccgaa
      901 ccaagcagat cgatcaggcg cttcatcgtg agcagataac cacgcagcgc gtaatcgcgc
      961 accgacagca gcggcaagcc gcccagatcc tcaatgccgg ccgtagtcgt gatgtattgg
     1021 aacaggtcag ggaagacttt aatcgtgacg cggccgcgct cgcaatagga aatgacgtgg
     1081 agtgtctcgc ggtggccttt ctccgggatg gcgacaatca cctcatcaat ccgatgctcg
     1141 ctgatcaggt ccggcaagtc ctcgggttta ccgaggatgg gcatgcccat aatctccgtt
     1201 tccggcgtgt cgccgttgat aatgccgacg agatcgtaac cgagaccggg tgaccaaacg
     1261 atacgctgca ggatcatccg cgccgtgtcg cccgtgccga ccagcaatac cctgtccttg
     1321 ccgataccgc gttcctggag ttgcctgcgc aaccagttgg tgaggaaccg ccccagcatc
     1381 aacaggatca tcgttagcag ccagttgtag accagcatcg tgcgcgggta atcgaggatg
     1441 gcgcgatcgt tgcggaagaa gatggtcgac agagcaaccg cgatcaggat acccactgac
     1501 acgctgatga agacgtagta gacctgatca atgcgcgagg cggcccgcgg aatgtaatac
     1561 tgccgattca aaaacagcaa aacgataata ccgaaggctt gcgccagaaa tagcgagaag
     1621 taatccgcca gcggcacctg attggccagc ggttcgggcc aatcgataga cacgcgtagc
     1681 tgataggcca gaacgaaggc cagcgcgatc atcaggaagt cgaggatcat gagccagacg
     1741 gtaaaaatag tgcggacacg ctgcgtggtc atgatacccg accctaatac tcctcaatcg
     1801 gcaacccgtt ctttgcctga ttcacatggc gcaagatcga ttcagcgccg ctgacggccg
     1861 tatcgccctg cgtgcccagc atggcggcca actcacgcac gcgaccaacc atatcaagcg
     1921 catcaacgtg ggtgatggtg cggccatcgg ccactgcctt gctgacatga aaatgaatat
     1981 cgccgtaacc ggccaactgc ggcaaatggg tgacgacaac cacctgatgg cccgcctcgg
     2041 ccgccaatcc ccacaacttg cggccgacca cgtcacctac gcggccgccg ataccctgat
     2101 cgatctcatc gaaaatcaac gtcggggtct gatccacctg ggccaatgcc gtcttcagag
     2161 ccagcatcag tcgcgccgtc tcgccgccgc tggccacgcg agccatcggc tttagcggct
     2221 ctccggggtt ggccgaaata aggaactcca cctggtcgat gcccgtctgg tcaaaggcca
     2281 ggcgacgctc ttcgccgtcc agcgacagga tcgcgccggc gggcgtatcc tgcgcctgga
     2341 aatcgacgcc aaagcgtgcg ccttccatct tgagatcggc caaatgcgtc tcgacggcgg
     2401 ccgacagttg ttccgcggca gcgcgacgct tctgcgagag cgcgccggcc attcggccga
     2461 tacgtcgcag gaacgtttcc tgttcggctt ccagttcggc cgtgcgcgtg ccgctattct
     2521 ccagcgtcct gagttcggcc tccgcttttt cagcggcagc cagcaacccg gcgacgctcg
     2581 cttcgccgta tttgcgcttc agatcgctga tcaactccag tcgtccttca actgcgttca
     2641 agcggcgcgg gtcatactca agcgcgtcca gataatcctg cagttcggcg gcaatttcgt
     2701 tcaattgaaa gccgatgccc tgcaaattcg acagagactc ctgccgcgtg tcatccagcc
     2761 gggctaactg agccatgtcg tgttccacgc ggcccaatag atcgatgacc gatgatcgat
     2821 cctcgtccac ggtggagagc aaggcgatgg cctcgcccgc aaatcgaccc aactgctcgg
     2881 cattggccag gcggatgcgc tcgacgcgca aactctcttc atcatcgggc ttcaacgcgg
     2941 cggcgatgat ctcctgaacc ttgaattgca gcatctcgat gcgttggttc atatcccggc
     3001 tgctgcggcg caacgcctcc agttcgcgct gtatggcatg gagcttgtgg acttcggccg
     3061 ccaactcccg acgctcgctc tccagtccgc cgaaagcatc aagcaacggc agatgggcgc
     3121 gcggccgcaa gagagacaaa tgctcgccct ggccatggat atcgatgagc ggatcggcca
     3181 cctgacgcag caccgagagg ctcacggttc gcccgttcac ccggcagata ttgcggccat
     3241 tggcgcgcat ctcgcgagcc agcacgacgt agtcgacacc ttcttcatcc aggccctcgg
     3301 tctccagcac cggccgcaag gtttcccgca aggtggcatc caactggaat gtcgcctcaa
     3361 cataggcttc gtccttgccg gcgcgaatca cggttgaatc ggcgcggccg ccgaggatca
     3421 gcgtcaccgc gtcgaggatg atcgatttac ccgccccggt ttcgccggtc aggacgttga
     3481 agccggggtc gagttgcagc cgcagctcgt cgatgatcgc gaagttgcga acaaacagtt
     3541 ccgaaagcat gtcaatcgaa tattagcgca tttgccagta agcggcactg gccccaacga
     3601 acaaatagat acccggcccc aacaaggcga acaggccgcc catattgccg gtcagaaccg
     3661 ccagcaggag cggcagcgcg gggatagcca cgccgaggat catcatggcc acgaccagcc
     3721 ccggcagata gcggccgcgc cgcccaccga tggctcgctt ggtgatgcgg ccgatgaagc
     3781 cccctaccgc gccgctgaga aagaagatca gcatgaaaaa caggaagccg cccctgaaaa
     3841 agttgaccat caagtagccg accagcaagc tgatgggaag cgaaaccagc acggcgatca
     3901 gataatccag cggtttgttg ttgaagaacg cgtcctccgc cacgcgccgg cattccgggc
     3961 agatgtagcc gaccggcgtc ttgatggcgc aactgatgca gatagggcga ttacaattgt
     4021 agcagcgcaa ggatgtggga cgatcaggat ggcgataaca cgccagctca taaacctcgg
     4081 cgtcgtgcga ctcttctgat tggttttcga cgatggtcgc ggggagcggg tcgtcggctt
     4141 cctcctcagc gttggccgcg gccagcgccg cctctacctc cgggggaatc ggtcggccgt
     4201 ccaggatagc cagcccggca atcgccgccg gcaaatcggg cgccaatgcc aacgcccgct
     4261 cataagccgc ccgcttttcg gcggggtcgt ggacgacctc ggccagtccc agccaggcct
     4321 cctcggcatc gggtgcttcg ccgagtatct gtcgatagat cgtctcggcc gccgcgttct
     4381 tgccgtaggt tgcgttgcgt tcggcttgcg tgagtaagga gcgtagccgg ggttctttga
     4441 tcgccataga gtagttccag gaaaatttcc gattatttca gagtcgcatc aagattaatt
     4501 ccacgagatt ttcatcaatt caggttaatg atcctgaaga gcggggtgag tgatgcctaa
     4561 ggcttctcat cccgactcag gccgatgttc aggtgcggcc aactgaagcc cagccgcccc
     4621 atcaaccggc gatagaaata gcctgcgctc tcgatgcgcg caaaatggca cacctgctca
     4681 ttcttggtga tgacgatctc gtcaccatcc tgcaccggtg tgcttttctg gccgtcggcc
     4741 gtcaggtaag cctcatgact catctccacc cggatcgaga tacgcgcgtt ctcatgcagc
     4801 accagcgagc ggttgaggct caaatgagcc gccaccggga caacgaccag attctgcaac
     4861 tgcggtggca tgagtggccc gccggcggcc atcgcatagg ccgtcgagcc ggtcggcgtg
     4921 gcgatgatca gggcgtcggc gatataggtc gtcacgagat cgccgtctac atgcaagtgt
     4981 aaatgaatga cacgagcctg tgccccgcgg ccgatgacca cctcgttcag cgccgaaaag
     5041 ggcatgaggc actcattgcc gcgccatagc tcggcgtgga gcagcagacg acgctcaatc
     5101 cagtattcgc cgcgcagcag gcgctccagc ttctccggcc agttctccgg cgtcgcctcg
     5161 ctcaaaaaac cgacccggcc gacgttgatc acgaatagcg ggatgccata gggaaccgac
     5221 cagcgcgctg ctcgcagcgt cgatccgtcg ccgccgagga caatcagcaa tgaggtgcct
     5281 tccagtggag cgttgaggtc ctcatcatcc atgggcgcgg ccgtccacga cggcacgccg
     5341 cgggctgaaa gccaatcgcg cacctccttg ctgagagggg cagattcagg gatgcggggt
     5401 tgttccagaa tgccgatgag gttcatgggc ggttgtgcat ggaaatatgg cgagcgcggc
     5461 aggagacgga gcaattcgtc atgcggccaa tcgttcccgc agccaggtga caatctcgct
     5521 cagaggaacc actgcttgct cctcgccctc aacgagcggc ctgacggcca ccacaccctg
     5581 caccagttca ttttcaccca tgatcagcac gtagcgcgcg ccatggcggt ctgcctcgcg
     5641 catctggctc ttcatgctgc gaccgcgccg ggcaaaggcc aggtgcgcgc cgatgcctgc
     5701 ctcgcgcagc cggaaagcca tcccgacggc ggagatcttc gtcgtcccgc caaaatgggc
     5761 caccagcacc ggcggcggta atacttccgg caagtcgacc ttttgctccc tcatgcccag
     5821 gacgatgcgt tcgatgccgc tgccaaaacc gacgccaggg gtcgatggcc caccgatgtc
     5881 ctcagccagg ccatcatagc ggccgccacc acacagcgca gactgcgcgc cgataccttc
     5941 ctgccacacc tcaaagaccg ttttggtgta gtaatcaatg ccgcgcacca ggcgaaaatt
     6001 gatggtgtag gactggttca gggcgtcaag cagctcgcgg acatcggcga aatgctcccg
     6061 acaatcgtcg cacaaatgat cggcgatatg gggcgcgccg gccaatagct cgtccatgcc
     6121 cggctctttg ctatccagca cgcgcagcgg attcttcgtc aggcgttcct tgtcaatggc
     6181 ggccaagcgg tcgtggtggt cacccagata ggcgaccagc ttctcgatgt agcccggctt
     6241 gcacaccggg cagccggtgc tattgagctg gaaggtcagg cccctgaacc ccaattcacg
     6301 gtagagattc atggccagca tcatcacttc aacatcggcc gccggatcct cttcgcccag
     6361 aatttcacag ttgatctggc tgtgctggcg caaacgtccg gcctgcggcc gctcgcgtcg
     6421 gaagctgggg ccgatggtgt agagttttac cggctggggc cagctgtgca tgccgttgga
     6481 gatgtaggcg cggacgaatc cggcggtgaa ttccggccga agcgtgatgc tgctgtcgtc
     6541 agcctcctga atggtgtaca tctctttctg gacaaaaaag tcggagccct gccccacgcc
     6601 acgcacaaat aactccgtcg cctcgaggat gggcgtatcg atatgctgga acccataacg
     6661 ttgcgtcagg cttccggcag cggcgataat cacgtcccaa tagcggcgat catcgggcag
     6721 aacgtcatgc atgcccgtca gccgctgaat ttgattagac aagttagcct ccacaaaaga
     6781 caggcagaat cacatagcct gtcgacggtg cgaatcgctt aattataccg ggaaaggggg
     6841 attaggaaac gtgcgacgag tggtcggtgg gggatcgtca ggcgagccgg cggtcgcaag
     6901 cgtcgcgaac gccgcaggcc gcgcagcggc ggcgatcggc atggtcgggt tcggggtcaa
     6961 aatcttcacg ggtggcccgc atctcatcga gcagatcgag cagatcggcc tccagctcgt
     7021 cggtgtaatc cacggcgacg gcgcgatccc tgtactggag aatgccatac ggcggccgaa
     7081 cgccataatt gacctccacc agcaggcaat aggccgccag ctgcaacacc tgcccatccc
     7141 atggcgaatc aggcgcgggg cctgatttca cctccacggg gatgattgat ccgtcacgct
     7201 gctccaccaa ataatccggc ttgccgacga gccgatactc cgaagcgtga aagacattgt
     7261 tgttggcccg ccaggcgccg gtgtcggtat agatgacgtt gccgggtggc aagccgctct
     7321 ctttccgcat gtcacggccg cggaaccaaa gtattgccgc cagaaacagc aggaacagca
     7381 ccaggatcag cggcagcccg ctcatcgtca tcgtctcact catcgcgcca tcgcctcatc
     7441 cgtcccatcc tcatcgggat aacaactcga agacgagcat ggcgacggca caaaacaggg
     7501 ctacataggc caggcgccgc agccacaccg actgttccac aaggcggccg tggcgttgat
     7561 gatattgcgt tcccgcctgc atttgggcga cgttggtcga agcgacggca cgcactcgac
     7621 gcagccacca ggagcgacgg caatagacat attcaccgat ttcactggct ctaatccatg
     7681 aatagctcat gcgttctatt ttattcgatt gaagggaagt gtcaaggaaa tggggcgatc
     7741 attcaatcgc gcggaaggtc gccgaaggcc ccagttgccg ataagccgcg gcgtagcgat
     7801 tgaggtaggc cccacagagg agaacgaggc tgctgatata agtccatgtg aggaacgcga
     7861 tgatcgtccc caccgagccg taaatcaggt tggagcgcga aagataggtg cccacaaagt
     7921 acaaaaatag ctgcttggcg aagtgccaca tgaggcccgc cataatcgcc cccgggatca
     7981 cttcgcgcca gccaatcttg acgtggggga tgaagtaata aagcagggaa aagaggagga
     8041 cgttcagaaa caccgcccag aaactggtgg taaacggccc gatgtccatc agcgtcgaag
     8101 ggacaaggct gttgatgatg gtcaataccg tgccctcaat ggatgaagcc aggagcaaca
     8161 acatcgtgat gatcagcacc atgccgaccg ccaatgtgcg gtggcgccac accgaccgcc
     8221 ggtgattgat atccgcgccc cagatgcgat ccatggcgcg ggtgacgacg ttgaacacgc
     8281 tggacgccga ccagatcagc atgatcgccg ccacaccggt gattggcccg cgcgcgcgga
     8341 tcaggttttg caggttttca ccgagcatcg tctccaggcc ggggatgatg aactccagct
     8401 cggctacaat tcgcgcttcg gccagcatgg ggtcaagcca gttactggcg atggcaacca
     8461 caagcagcgc cagcggaaaa acagagaaca atgtgaagta gccgatcgac gacgcgacca
     8521 gttgggactc cggggccaga gcatcctgaa tagagcgcag ggtcagtcct ccccacagct
     8581 tgatggcggg taaaaacccg gttctaacct gagatataag cccgaggggc gatttttgta
     8641 attgattcag gacattaccc atttattcag ccaactgatt gacccaggga agtataatct
     8701 catgcctgtc attgcaccaa attatcgccc cgcggccgaa tacgtcgccc gaaagaagcc
     8761 ctcctgtttg agaataatca gcggccgtgc tactatttac ctacatgaaa cggcccgaat
     8821 ctctgcgcga atatcgctgg cttgtagctc cggaaccccc gcccggctcc cctctacaca
     8881 caattggcga taccatccac ccggtcttac gccaggtcct gtataaccgc gggctgaccc
     8941 agccgggaca gattcaatca tttctcgaaa accattacct tcaatcgcgc gatccgttcg
     9001 ggctgcccga catggagcct gccgtcgagc gcatcacccg cgccctgcgc gatggcgaaa
     9061 tgattgccgt ctacggcgat ttcgatgccg acggcgtcac cgccaccgtc ctcctgacgg
     9121 aagcgctgcg ctccatggtc gccgatcgcc gccagatcat accctacatt cccgaccgat
     9181 tcgacgaggg atatggcctg aatcttgatt ctctggccaa attgcgcgcc aaaggggtca
     9241 gcctggtgat cagcgttgac tgcggcattc gctccccggt cgaggcagca cacgcccggt
     9301 cgattggcct ggatctgatc atcaccgatc accacggcct gggccgagaa ataccgccag
     9361 ccgtggccgt catcaacccc aagcgcccgg actccaccta ccccgagcag atgctggccg
     9421 gcgtcggcat cgctttcaag ctggcccagg cgatgcgcca gtttgcgccc gatctcgtaa
     9481 ccggcgacga atcaaatctg ctcgatctgg tggctatcgg cacagtggcc gacctggccc
     9541 ccctcctggg cgaaaatcgc aagctcgtgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn ngcattggcc gaagggatgg tggatcccga agcgccaatc atcatcgctc
     9721 gcaatacgga atttaattcg ggcattgtcg gcctcgtggc cggccgcctg gccgaaaaga
     9781 attatcgccc ggctatcatc atggaagagg gcgaggaaga aagtcgcggc tcctgtcgct
     9841 cgatccccga attccatatc accaaagcgc tcgatgaggt ggccgatctg ctggttcgcc
     9901 acggcggcca cgcgcaggcg gccgggttca ccattcgcaa cgaacacgtg ccggagttca
     9961 tcagccgtat gacccgcatc gccggcgagt ggctggatgg gcatgatctg cggccgagtc
    10021 tcgccatcga tgccgacttg ccgctaagtg acatcgactg ggcactgcag ggaaccatcg
    10081 aacagttgga gccgaccggc ttcgagaacc ccaaaccctt gctgctcagc cgggccgtgg
    10141 aagtcgtcag tcatcgccct gtgggcacgg acggcagcca tctgcaactc cacgtcgtgg
    10201 atgctcgtcg caatggagcc gatggccata gcggcgcgaa acggcaaccg gcacggccgt
    10261 atccggcaat cgcctttcgc caaggcgaat gggccgcgca catgccgcgc tatgccgaca
    10321 tcgtctatcg attgggcgtc aaccattggc agggaagcgc caacctgcaa cttgtcatcg
    10381 aagatattcg ccccacggaa gaagagagta tagccgtatg accattctcg ttaccggcgc
    10441 ggccgggttc ataggcagcc atttggcgga acgcttgtgt cgccgaggcg accgcgtcat
    10501 tgggctggat aacttcaatg attattacga ccccacccgc aagcgcgcca acgaacaacg
    10561 gctaaacacc taccccaact ttcgcatggt cgaggccgat ctgcgcgacc ggacggctct
    10621 attcgacctg tttgcgaccg aaaagatcga tgctgtggcc catctggcgg ccatggccgg
    10681 cgtccgcgac gccattaagt ttcccaatct ttatgtcaat gtcgatctga acggcacaca
    10741 aaacgtcctc gacgcggtgc acgccgtcgg cggggtagca aatttcgtct ttgcctccac
    10801 ctcgtcggtc tatggtaaca cgacgcaagt cccctttgtg gagaccgatc cctgtgaccg
    10861 cccgctccag ccctatgccg ccgccaagcg cgcggccgaa atactcggct acacttacca
    10921 ctacctgttc ggccagaact tcactgtact acgttttttc accgtctatg gacccaacgg
    10981 ccggccggat atgatggcct acctcctggc cgacagcctg atgaaaggca gagagatacc
    11041 tctctatgac aacggccaga tgtatcgcga ctggactttc gtcgacgaca cggtcagcgg
    11101 catcgtagcg gccatcgacc ggccattggg ctacgagatc atcaatctgg gtcggggcga
    11161 gccggttctt ttgcgcgatt ttgtcgagat catggaagaa ctgagcgggc gcaaggccaa
    11221 cgtcgtgccg acgccccggc tggcggccga tttcgtgcgc aatgaagccg atatcagcaa
    11281 agcccgccgc ttgctggatt atgaccccca agtctctgta gccgatgggg tggaacgatt
    11341 ctggaaatgg taccaacgct atgatgccgg gcaaggataa cgcaccgccc gcgcttggcc
    11401 cgttccatta tgtggccaac gaagccaaat ggacggcctg cctgcgcgat cttcaggccg
    11461 cgccgcgtct ggccatcgac ctggaagcca acagcatgtt cgcctaccgg gaaagggtat
    11521 gcctgatcca ggtctccacg gccgacaccg actacatcat cgaccccatc cagcacctcg
    11581 atctgagcgg cctgggagca atcatcgccg acccgcgcgt ggaaaaggtg ttccacgcct
    11641 ccgagtatga tctcatcctg atgcggcgcg actacgactg gcatctggac aaccttttcg
    11701 acacgatgtg ggccgtgcgc atcctgggct attcgcaaat ggggctggcc agcttgctgg
    11761 agaatttctt cggcgtcagc accagcaagc gcttccagaa ggccaactgg tgcaagcggc
    11821 cgctttcatc ggccgaattg gcttatgcgc aaaccgatac ccactacctg ttacccctgc
    11881 gcgatcgcct ggcggccgag cttaaagcgg tcgaccgatg ggaagaagcg accgagattt
    11941 tccgggaaca ggcgcaggtt cgtctgccca ataataactt cgatcccgac ggcttctggt
    12001 tcatcaacgg cgcgttcgat ctgccgccgg agaaacaagc ggcgctaaga ggcctgtatc
    12061 ttttccgcga tcgcgaagca aaacgccgcg acgtcccccc attcaagatt ttgggggaac
    12121 gaacccttgt ggagctggcc gaaaaattac cggggcga
//
LOCUS       DAOMQG010000066        12117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2276_length_12117_cov_10.663074, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000066 DAOMQG010000000
VERSION     DAOMQG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2276_length_12117_cov_10.663074"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PLC06_05635"
     CDS             <1..174
                     /locus_tag="PLC06_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HRO89961.1"
                     /translation="RTSGELRISNFLIWQAAYSEYYPTPALWPDFGREDLYEAIVTFN
                     KRERRYGLVTDAS"
     gene            192..1019
                     /locus_tag="PLC06_05640"
     CDS             192..1019
                     /locus_tag="PLC06_05640"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRO89962.1"
                     /translation="MFTQRLIVTLIVGPVMLIATYLGGWYFFAPVLFLMLVAGWEYAN
                     IMRVLDHKLPLWLLLPAITLLLVGAWRPELNLTNVTLFLGTFIGLIYCLALFERGNTL
                     DAALNWFALIAGIILLGWVGSHFFLLRGLPENGWQWTTVALMGIWSADMAAYAVGKWM
                     AGRWGLGRHPLAPRLSPKKTVEGYVGGIVAGTGIAIATAAVLGLSLALAAIAGLAVSI
                     LGLFGDLSISMLKREAGIKDSGRIFPGHGGALDRSDSLIWAIAIVYYLVVFLGPLFP"
     gene            1073..2086
                     /gene="fbp"
                     /locus_tag="PLC06_05645"
     CDS             1073..2086
                     /gene="fbp"
                     /locus_tag="PLC06_05645"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRO89963.1"
                     /translation="MTTVTTIERFILDRQPEHARGELTYLLYDIALAAKIIASKTNRA
                     GLVDILGEAGATNVQGETQQKMDVFAHNIVRQLCDHTGRLCLMVSEEQEEPLYIPAKY
                     DKGNYVLVFDPLDGSSNIDVNVSIGTIFGIYHCLDESRRGRIEDALQPGKDLVAAGYV
                     LYGSSTMLVYSTGDGVHGFTLNPEYGEFLLSHPNLTLPDPPAYYSVNVAYYNRWSPSV
                     QRFVDWLHGTGEDAPSLSARYIGSLVADFHRNLLRGGIYCYPAESTRADGKLRLLYEA
                     APLAFLMEHANGYASDGRRPILEIVPEHLHQRTAFFIGNRKLVEQAERFIRQGVDEAA
                     VSD"
     gene            2441..3718
                     /gene="rho"
                     /locus_tag="PLC06_05650"
     CDS             2441..3718
                     /gene="rho"
                     /locus_tag="PLC06_05650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRO89964.1"
                     /translation="MDIGDLESKKLSELRDIGRDMKIPGYTTMKKQDLVFRIMQADAE
                     SGGFHFRGGVLEIVEDDKQTMGFLRSGSYLPSKDDIYVSNSQIRRIGLKTGDMVYGQV
                     RVPKDNEKYYSLLRVEAVNGMSPDQLKNRVRYESLTPIFPDQKLKLETNPNILSTRLI
                     DLITPIGRGQRGLIVSPPKAGKTTVLKEIANGISENYPEVHMMVVLIGERPEEVTDME
                     RSTQGEVVSSTFDEPVKNHCRVAEMALERAKRLVESGRDVVILLDSITRLARAYNLTV
                     PPSGRTLSGGLDPGALYPPKRFFGTARNLEFGGSLTIIATTLVDTGSRMDDVIYEEFK
                     GTGNMELMLSRRLQERRIFPAFDIERSSTRREELLMSADELQRVYTMRRMLGHLMDTP
                     GYDISSATAAVLQRLRATRTNYEFLETLTKDMA"
     gene            3892..4956
                     /locus_tag="PLC06_05655"
     CDS             3892..4956
                     /locus_tag="PLC06_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRO89965.1"
                     /translation="MQIEKGTWQRAAGYIVLVLIAGLIVLGWRVGIAGGGNTSIGEQP
                     VSATPASVAAAGTVAGDPPAVEAAPVVADAPVLVDAALVPAAVPHTFVGHKPEHEFVV
                     YRVERGDTPNGIADKFGIKATTLLGGNPLLSQESSLLQTGIDLVILPVDGVLHDVAPG
                     DTLESISQQYSVPVETIVGYGPNNLEFPYRLYPETQVLVPGAVREVFVWTPPSLESVR
                     GRSTGSGVTPLIVGTGTFIYPVGSRNFTQYFWYGHPGVDIALPEGTAVVASDTGTVTF
                     AGWNIYGYGNLIVVNHGNGYETFYAHLSGISVVPGQIVYQGNVIGSTGNTGNSSGPHV
                     HFEVRINGAQDNPCWYIGGC"
     gene            complement(5058..6113)
                     /gene="rlmN"
                     /locus_tag="PLC06_05660"
     CDS             complement(5058..6113)
                     /gene="rlmN"
                     /locus_tag="PLC06_05660"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRO89966.1"
                     /translation="MINLYDLSLPELTALLAEWGQPAYRARQVWEWLYHHYAGTFDEM
                     STLPGALRERLASETTLTIGEIALSRNSSDGQTRKVLFQLPDGQYIETVLMRYEKRRT
                     LCISTQAGCAMGCVFCATGQMGFMRHLSVAEIVGQVMYFARELAAEDQHVTNIVMMGM
                     GEPLHNYDNTLATVDRLTDATGFNLGARKITISTVGLIPAIRRYADEQRQTPLAVSLH
                     AATDEERGRLLPVNRRWPLAELMEACRYYVAKTGRRMTFEWALIAGENDTAEQAHKLG
                     ELLQGMLGHVNLIPLNPTAGYGGTPSSPERVARFQEILTEYGVTSTVRVRRGIDIQAG
                     CGQLRDRHLADIGGRSG"
     gene            complement(6121..6960)
                     /locus_tag="PLC06_05665"
     CDS             complement(6121..6960)
                     /locus_tag="PLC06_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRO89967.1"
                     /translation="MNKPIKNLILDMDGVLWRGDTPMPGLVDFFDTLRAADIGFILAT
                     NNATKTVMMYTERLARFGVQVLPQQILTSAEATASYLVGRFPPGSAVYVIGAKGLHDA
                     ISARGFHILSVADVHLEAKSSAPITAAAVVAGFSPNMTYQDVAMGAHLINQGVPFIGT
                     NPDATYPTEIGPMPGAGATLAMLIAATSVQPVIIGKPGPVIFQEAVERLGGDPSAVAM
                     VGDRLSTDIQGAKAAGLQAILVLSGISTREEAETGPVHPDYIFADITELAAFLTQERA
                     VAH"
     gene            7167..7856
                     /locus_tag="PLC06_05670"
     CDS             7167..7856
                     /locus_tag="PLC06_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO89968.1"
                     /translation="MKVSVIIPVYNEFATVEPAVRAVLAVDEADEIIMVDDGSTDGTR
                     DLYPAIQALDESIKVILHEKNMGKGAAVRTGFEHATGDIFLIQDADLEYDPRDYPALL
                     RPIVEGRAAVVYGSRFRGGPTKTMFFWHMVGNKMLTFVTNIVYNTILTDMETCYKVFR
                     ADVVRGIPLRSRGFEFEPEITSKVLKRGHRIYEVPISYNGREFDEGKKLNPWREGPKA
                     LYYLLKYRFVD"
     gene            7849..9345
                     /locus_tag="PLC06_05675"
     CDS             7849..9345
                     /locus_tag="PLC06_05675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO89969.1"
                     /translation="MIRTTSDESQASDGRSLAVTCGVWAVTCLGATLRFHGLFANSFH
                     ADEALFATWARLIAVWRDPLLLTQAVDKPPLPFYLQAIFYPLFGPVEWAARMPAFIAS
                     LLLIPLVAQLARRLTGNRAAALPAAVFVAMAPLAIQFSATAFIDPLLTFWLAAALYVM
                     AAPKSESASRRHGLLAGFLFGLALATKYQAILFVPLLVGVGWLGNWGRREWLQGIAGM
                     SAALLALLIWQWTRSGDMVALQWANIGGIRPARSWELWPRAVATVQLWRLAAGWPLVV
                     LSAVVVGALLWIKRGRFRLSSMDSLLILFISGYILLHWLWAVPIWDRYLLPVVPLVAV
                     LFGRVMSALWSATGQRMRPQGAAAAASLMILLLMPTALVARAGQFPIGGRPGADGGAS
                     RIALLLKDAPYGAVLYDHWYSWHWQYQLFDGRVYVSWFPHADALIEDLMVFAGGGSPR
                     YLVLPSSAVGAPVARQLSKNGYVLEKVGGQEEAAGMTLYRILPGRSTE"
     gene            9342..>10921
                     /locus_tag="PLC06_05680"
     CDS             9342..>10921
                     /locus_tag="PLC06_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89970.1"
                     /translation="MSAFGRTAVVGRLSRLAVPVALVALVLLFFNKMAFSNLILARGD
                     TFLYFYPYWTAAAEALRSGRVPLWNPDIFMGAPMLANSQVGFFYPLNWPIWLFLPTPY
                     AVSASILIHLSIAGLGAYLAGRRSMGLGRAAALFAAALFALGGYLTAQVEHINQLQGL
                     AWLPWYFAVLTPVVTGDGARQWRTLMRGALGVGILFALQLLAGHTQTAFISGVAISLW
                     ALFHWLTDARAAGRGIGVLARAGLVIGAGVALAALLAAVQLLPTLELAGQSSRQGGLS
                     LNEVLSFSWHPLHMTRALLPAYGAALFSEYVAFLPLTALALAVVGTWGRRKRPGVLPW
                     VLLALMMILLALGRFTPVYYLLGRVPGFDLFRVPARWLAVAALAVALLAGHGWERLRR
                     FAESGIPTDERREEAREAFGRPLAVARTGLLLLIAWGYVAGWVARWVPTGAEAPFETP
                     ALLTLLGWLIEFVLASLLLWVILTAPSERARRATTELVILALAVLWLGSRSLPYNQLT
                     TLEAYFDWRPPQARLAALA"
     assembly_gap    10922..11021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11022..11995
                     /locus_tag="PLC06_05685"
     CDS             <11022..11995
                     /locus_tag="PLC06_05685"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRO89971.1"
                     /translation="TADGRLREYLTAAPDARWLSLFNGRYLVTDKTGDLWREGVFFDR
                     QHPTRIGAEPVLLADVPTYEATELWMLAEAPPEVEVITAEGESWRLTPELWQAPDLYR
                     LDYPRAATAERVTLLPCPEQAAECGVNALTLVDARDETFLPLTMPPYRLIFSGDVKIY
                     ENLDALPRAFLVHEWQVASDTVAAVAMMRAGDFEPGLRAVVTGENVPSSSATAGRGSA
                     TIVDYRPEVVTLHTEADAESFLILTDAIYPGWEAAIDGVPVSVWSVDGLFRGVFVPAG
                     DHEIVMSFRPDSYRRGLGLSAAGILMLIVLGLLLYQNRERTIPASTI"
BASE COUNT         2124 a         3413 c         3777 g         2703 t
ORIGIN      
        1 cgcacgagcg gcgaactgcg aatcagcaac ttccttatct ggcaggcggc ctattcggag
       61 tattacccca ctccggctct ctggcctgat tttggccgtg aagatcttta tgaggcgatt
      121 gtcaccttca acaagcggga gcgccgctat ggacttgtca cagatgcatc ctgattgcgt
      181 tgcctgatcc gatgttcacg caacgcctga ttgtgaccct cattgtgggg ccagtgatgt
      241 tgatcgccac ctatctgggt gggtggtatt ttttcgcccc ggtgttgttc ctgatgcttg
      301 tggcggggtg ggaatacgcc aatatcatgc gcgtgctgga tcacaagttg ccgctttggc
      361 tgttgctgcc ggccatcact ctactgctgg tgggggcgtg gcggccggag ctgaacctga
      421 cgaatgtgac ccttttcctg gggacgttca tcggcctcat ctattgcctg gcgctctttg
      481 agagggggaa tacccttgat gcggctctga actggttcgc cctcatcgcg ggcatcatcc
      541 ttttggggtg ggttggcagt catttctttc tcttgcgcgg cttgccggag aacggatggc
      601 aatggacgac tgtggcgctc atgggcatct ggtcggccga tatggccgca tacgctgtgg
      661 gaaagtggat ggccggacga tggggattgg ggcgacaccc tctggcccca cgtctcagcc
      721 ccaagaagac ggtcgaagga tatgtcggcg gcatcgtggc tggaacgggt atcgctatcg
      781 ccacggccgc ggttctgggc ctgtcgcttg ctttggcggc cattgccggg ctggcggtca
      841 gcatcctggg tctgttcggc gatttgagta tttcgatgct caagcgagag gccggaataa
      901 aagactccgg ccgaattttc ccggggcacg gcggagcgct ggaccgttcg gattcgctga
      961 tctgggccat cgccattgtc tactatctgg tcgtctttct ggggccgctc tttccatagt
     1021 gtgcctctcg ggctttatca tccactgagt cagatctaaa ggagcgcggg ccatgactac
     1081 tgtgaccacc attgagcgtt ttattcttga tcgtcagccg gagcatgcgc ggggcgagtt
     1141 gacttatttg ctatatgaca tcgccctggc ggccaaaatc atcgccagca agaccaaccg
     1201 cgccggactg gtggacatcc tgggcgaggc cggagccacg aatgtgcagg gcgagacaca
     1261 gcaaaagatg gatgtttttg cccataacat cgttcgacag ctctgcgatc acaccggccg
     1321 cctatgcttg atggtgtccg aagagcagga agagcccctc tatatccccg cgaagtatga
     1381 caaagggaac tacgtcctgg tcttcgatcc gctcgatggc tcgtcgaaca tcgatgtgaa
     1441 cgtgagcatc ggcactatct tcggcatcta ccactgcctc gatgaaagcc ggcgcggccg
     1501 cattgaggac gctttgcagc cgggcaagga cctggtcgcg gccggttatg tcctgtatgg
     1561 ctccagcacg atgctggtct atagcaccgg cgacggcgtg catggtttca cgctcaaccc
     1621 ggaatacggc gagttcttgc tgtcccatcc taacctgact ttgccggatc cgccggctta
     1681 ttacagcgtg aacgtggcct attacaatcg ttggtcgccc agcgttcaac gatttgtaga
     1741 ttggcttcat ggaaccggtg aggatgcgcc gagcctgtcg gcacgttata tcgggtcgct
     1801 ggtggccgat ttccaccgca atttgttgcg cggcggaatc tattgttacc cggcagaaag
     1861 tacccgcgct gacggtaagt tacgccttct ttatgaggcc gcaccgctgg ccttcctgat
     1921 ggagcatgcg aatggttatg catccgatgg tcgccgcccg atcctggaga tagttccgga
     1981 gcacctgcat cagcgaacgg cattttttat tggaaaccgg aagcttgtgg aacaagccga
     2041 acgctttatt cgtcagggag ttgatgaagc tgcggtatcg gattaggtcg aggtggagcg
     2101 attgatacca acgtttagct tattgaccag agtatattga catgttatac tacccgcgtc
     2161 ccaccttatg gaccgcgctg tgggaaagaa aagctccctc tattgaaaga aaacaaataa
     2221 cgatatttct atcgaattgc ccggcttggc ttccctgttc tttataaccc gtggtttatg
     2281 acatccaagg atgtcgatat tcccgatcgg cctgttgtgc gggggcagta tcgcaggagt
     2341 tgggttaggc caaaaacaca cacttttgaa cgaagttaaa atctaacttg tggcatgctt
     2401 gttccgtata gggattacgt gtgccaaaga gaaacaacgt atggatattg gtgatctgga
     2461 aagtaaaaaa ctgagcgagc tgcgcgacat cggccgggat atgaaaattc ccggttatac
     2521 cacgatgaaa aagcaggacc ttgtctttcg tatcatgcag gccgacgccg agtcgggcgg
     2581 gttccacttt cgcggtggcg tgctcgagat cgttgaagac gacaagcaga cgatgggctt
     2641 cctgcggtcc ggctcctacc tgccgagtaa ggatgacatc tatgtctcca attcgcagat
     2701 tcgccggatt gggctgaaaa cgggcgacat ggtctatggc caggtgcgcg tgccgaagga
     2761 taatgagaag tactacagcc ttctgcgcgt cgaagccgtc aatgggatga gtcctgacca
     2821 gctcaagaac cgggttcgct acgaatcgct gactcccatc ttccccgatc agaagctcaa
     2881 gctcgaaacc aaccccaaca ttttgtcgac acgcctgatc gacctcatta caccgatcgg
     2941 ccgcggtcag cgaggcttga tcgtttcgcc tcccaaggcc ggcaagacga ccgtccttaa
     3001 agagatcgcc aacggtattt cggagaacta tcccgaagtt catatgatgg tcgttctcat
     3061 cggcgagcgc ccggaagaag tgaccgacat ggagcgctcg acgcagggtg aagtggtgag
     3121 ctccaccttc gacgagccgg tgaagaacca ttgccgcgtg gccgagatgg cgctggagcg
     3181 cgccaagcgc ctggttgaat cgggccgcga tgttgtcatt ctgctcgact ccatcacacg
     3241 cctcgctcgc gcctataact tgaccgttcc ccccagcggc cggacgctgt ccggtggtct
     3301 ggatcccggc gcgctctatc cgccgaagcg gttcttcggc acggcgcgaa acctggaatt
     3361 cggcggtagt ctgacgatca ttgccacgac gctggtagac accggcagcc gcatggatga
     3421 tgtcatctac gaagagttta agggtacggg caacatggag ctgatgctca gccgtcgctt
     3481 gcaggagcgt cgtatcttcc cagccttcga tattgagcgc tcgagcactc gccgcgaaga
     3541 attgctcatg tcggccgacg aactgcagcg cgtctacacg atgcgtcgta tgctgggtca
     3601 tctgatggac acccccggct acgatatcag cagcgccacg gcggccgttc tgcaacgcct
     3661 gcgagccacc aggaccaatt atgaattcct ggaaacgctc accaaggaca tggcctaatc
     3721 aatcgttatc tcggcttgac aggtggacgg ccccggcttt tgttgctgta aagagacgcg
     3781 gaccgcccac cggtcaggtc tgtcaagcta agcgcttgta gagaaagcaa ctattcgagc
     3841 catatccttg ttgcttcgcc ctccacaggt ggatagcatc caccgttgcg tatgcaaata
     3901 gaaaaaggca cctggcagcg tgctgccggg tatatcgttc ttgttctgat agccggactc
     3961 attgtcctgg ggtggcgagt tggaattgcc ggcgggggga atacctcgat cggcgagcag
     4021 cctgtttccg caacacctgc ttcggtcgcg gcggccggca cggtcgccgg cgatcccccg
     4081 gctgtcgaag cggctcccgt tgtggcggac gcgcctgtcc tcgtcgatgc ggccctggtc
     4141 ccggcggccg ttccccatac cttcgtcggt cacaagcccg aacatgaatt tgtggtttac
     4201 cgcgtcgagc gcggcgacac gcctaacggc atcgccgaca agttcggcat caaggcgacc
     4261 acgctgctgg gcggcaatcc attgctgagt caggagtcga gccttttgca gaccggcatc
     4321 gatctggtta tcctgccggt tgacggcgtg ctccatgacg tggctccggg cgacacgctg
     4381 gagagtattt cccagcaata cagtgtcccg gttgagacga ttgtcggcta cggcccgaat
     4441 aacctggagt tcccctatcg cctttacccg gaaacacagg ttctggttcc cggcgccgtg
     4501 cgcgaggtat tcgtgtggac gccgccaagc cttgaatcag tccgcggccg ctccacgggc
     4561 agcggcgtca cgccgctcat cgtcggcacc ggcacgttca tctatcccgt cggctcgcgc
     4621 aacttcaccc agtatttctg gtacggtcat ccgggcgtcg acattgcttt gcccgaaggg
     4681 acagccgtcg tggcttccga taccggcact gtgacttttg ccggatggaa catctatggg
     4741 tatggcaatc tgattgtagt caatcatggc aacggatatg agacatttta tgctcatttg
     4801 agcggcatca gcgtcgttcc ggggcagatc gtctaccagg ggaatgtcat cggttcgacc
     4861 ggcaataccg gcaactcctc tgggccgcat gtccactttg aggtgcgcat caacggcgcg
     4921 caggacaacc cttgctggta cattggcggc tgttagcgaa aggcctcaac tgcaataagc
     4981 tccgataaac aaacgcggag cgcccgggat actattccgg cgctccgcgt ttttattgat
     5041 gagttcggct atcggcttta accggatcgc ccgccgatat ccgccagatg cctgtcgcgc
     5101 aattgcccac aaccggcctg gatgtcgatt ccgcgtcgaa cgcgcacggt gctggtgacg
     5161 ccatattcgg tcaggatttc ctgaaaccgg gccacgcgct ccggcgagga aggcgtgccg
     5221 ccatacccgg cggtgggatt gagcgggatc aggttgacgt gccccagcat cccttgtagt
     5281 agttcaccca gtttgtgcgc ttgttcggca gtgtcgtttt ctccggcgat gagcgcccac
     5341 tcgaaggtca tccggcgacc ggtcttggcc acataataac ggcaggcttc catcagttcg
     5401 gccagcggcc agcgacggtt gaccggcaac aggcgaccgc gctcctcgtc cgtggcggcg
     5461 tggagcgaga cggccagcgg agtctgccgc tgctcgtcgg catagcgacg gatggccggg
     5521 atgaggccga cggtggagat ggtgatcttt cgcgccccaa gattgaaccc cgttgcgtcg
     5581 gtcaatcggt cgactgtggc cagggtgttg tcgtagttgt gcagcggctc gcccatgccc
     5641 atcatgacaa tattggtcac atgctggtct tcggccgcca gctcgcgggc gaagtacatc
     5701 acctggccga cgatctcggc tacggacagg tggcgcataa accccatttg ccccgtggca
     5761 caaaagacac agcccatcgc gcagcccgcc tgggtgctga tgcacagggt gcggcgcttc
     5821 tcatagcgca tgagcaccgt ctcgatgtac tggccgtcgg gcaattggaa caggactttt
     5881 cgggtctggc cgtcggacga gttccggctg agcgcgatct cgccgatcgt cagggtggtt
     5941 tcgctcgcca gccgttcgcg cagtgcgccg ggcagcgtgc tcatctcgtc aaatgttccg
     6001 gcgtaatgat gatagagcca ctcccatacc tgccgcgccc gataggcggg ttgtccccac
     6061 tcggccagca gagcagtcag ttccggcaag gataaatcat agagattaat catagtattg
     6121 tcagtgggca acggcccgct cctgcgtcag gaatgcagcc agttcggtga tatcggcgaa
     6181 gatgtaatcg gggtgaacag gcccggtctc ggcttcctct cgggtcgaga tgccgcttag
     6241 aacaaggata gcttgcaaac cggccgcctt ggccccctgg atatccgtgg acaaccgatc
     6301 gccaaccatg gctacggccg acggatcgcc gccgaggcgc tcgaccgctt cctgaaagat
     6361 gacaggcccc ggtttgccga tgatgaccgg ctgcacgctt gtggcggcaa tgagcatggc
     6421 cagtgtggct ccggctcccg gcatgggacc tatctcggtt gggtaggtgg catcagggtt
     6481 cgtgccgatg aagggcacgc cctgattgat caaatgtgct cccatggcca catcctgata
     6541 ggtcatgttg ggcgaaaacc cggcgaccac ggctgcggcc gtaatcggag cggaagactt
     6601 ggcttcgaga tgaacgtcag cgaccgacag gatatgaaac cctcgtgccg atatggcatc
     6661 gtgcaggcct ttggcgccga tgacgtagac ggccgaaccc ggcggaaacc ggccgaccag
     6721 ataagatgcc gtggcctcag ccgaggtcag gatttgttgc ggcaacacct ggacaccaaa
     6781 acgggccagt cgctcagtgt acatcatgac cgtcttggtg gcgttgttgg ttgccaggat
     6841 gaagccgata tcggccgcgc gaagggtatc gaaaaaatcg acaaggccgg gcatcggcgt
     6901 gtcgccgcgc cataataccc catccatatc gagaatcagg tttttgattg gtttgttcat
     6961 aatcattgca tctgttcgtg ggcgcgaaac ggcgggcgaa caatcaaaag attatagccg
     7021 ataaagagcg cagggtcagc agcggtggat cgccggttgg agaggcggaa tggtgatgtt
     7081 ataattcaca agttgcccgc ccgtcgggag acatttttgc cgaataaaat aagcgcgacg
     7141 cgccctgtgg gagaaaatct gcacaagtga aagtctcggt cattatcccg gtatacaatg
     7201 agttcgccac ggttgagccg gccgtcaggg ctgtgctggc ggtcgatgag gcggacgaga
     7261 ttatcatggt tgacgacggc tcaaccgatg ggacgcgcga tctctacccg gccattcagg
     7321 cgctcgatga gtcgatcaag gtcatcctgc acgagaaaaa catgggcaag ggcgcggccg
     7381 tgcgtaccgg ttttgagcat gccaccggcg atatcttcct cattcaggac gcagacctgg
     7441 agtatgatcc ccgtgattac cccgcgttgc tccgaccaat tgttgaaggg cgggcggccg
     7501 tagtctatgg ttcccgcttt cgtggcggcc cgaccaagac gatgttcttc tggcacatgg
     7561 tcggcaataa aatgctgacc ttcgtgacca atatcgtcta taacacgatc ctgaccgata
     7621 tggagacgtg ctacaaggtc tttcgggccg atgtggtgcg gggtatcccg ctgcggtctc
     7681 gcgggtttga gtttgagccg gagatcactt ccaaggtgct gaagcgcggc catcgcatct
     7741 atgaggttcc gatctcctac aacggccgcg agttcgatga gggcaagaaa ttgaacccgt
     7801 ggcgcgaggg gccgaaagcg ctctactatc tgttgaaata tcggtttgtt gattaggacg
     7861 acaagcgacg aatcacaggc ttcggacggc aggtctcttg ctgtgacctg tggcgtgtgg
     7921 gctgtcactt gtctgggagc cacgctccgt ttccacgggc ttttcgctaa cagcttccac
     7981 gccgatgagg ctctcttcgc tacctgggca cgactgatcg ccgtgtggcg tgatcccctt
     8041 ctgctgaccc aggctgttga taaaccccca ctcccgtttt acctgcaagc gattttctat
     8101 cctctgttcg gcccggtgga gtgggcggca cggatgccgg cgttcatcgc ttcgttgctg
     8161 ctcatcccgc tcgtcgcgca actggcacgc cgtctgaccg gcaaccgcgc ggccgctttg
     8221 ccggcggcgg tgttcgtggc catggctccg ctggccatcc agttcagcgc caccgccttc
     8281 atcgatccgc tgctgacctt ctggctggcg gcggctttgt acgtgatggc ggcgccaaag
     8341 tcggaaagcg cctctcgccg tcacggcctt ctcgccggat ttctctttgg gctggccttg
     8401 gccacaaaat accaggcgat cctgtttgtt ccattgctgg ttggggtggg gtggcttggg
     8461 aattgggggc ggcgcgagtg gctgcagggg atagcgggca tgtcggccgc cctgttggcg
     8521 ctattgatat ggcagtggac cagatcgggc gatatggtgg cgctgcaatg ggccaatatc
     8581 ggcgggattc gcccggctcg ttcgtgggag ctatggccgc gggccgtcgc gacggtgcag
     8641 ctctggcggt tggcggcggg atggccgttg gtcgtgctaa gcgccgttgt ggttggagcg
     8701 ctgctatgga ttaagcgcgg gcgcttccgg ctctcgtcga tggattccct gctgatcctg
     8761 tttatttcgg gctatattct gcttcactgg ttgtgggccg tgccgatatg ggatcgttat
     8821 ttgctgcccg tcgtgccgct agtggccgtc ctgttcggcc gagtgatgag cgctttgtgg
     8881 tcggccaccg ggcaacggat gcggccgcaa ggggctgccg cggcggcgtc cctgatgatc
     8941 ttgctgctca tgcccactgc gttggtcgcg cgcgcggggc aattcccaat cggcggccgg
     9001 ccgggagccg acggcggcgc gtcgcgcatc gccctgctgc tgaaagatgc cccttacggc
     9061 gctgtcctgt atgatcattg gtatagttgg cactggcaat accagttgtt cgacgggcgg
     9121 gtgtacgtga gttggtttcc ccatgccgat gctcttatcg aagacctgat ggtgtttgcc
     9181 gggggtggtt ccccgcgcta tctggtcttg ccgtcgtcgg ccgtcggcgc tccggtagcg
     9241 cggcagttgt cgaagaatgg atatgttctg gaaaaggttg gcggtcagga agaagcggcc
     9301 ggcatgacgc tttaccgcat cctgcccggg agatcgaccg aatgagcgcg ttcggccgca
     9361 cggcggtggt cggccggctc agccgcctgg ccgtgccggt ggctttggtg gcgcttgtcc
     9421 tgttgttctt caacaagatg gccttcagca atctgatcct ggcgcggggc gataccttcc
     9481 tttactttta tccctattgg acggccgcgg ccgaggcgtt gcgcagcggc cgagtcccgt
     9541 tgtggaaccc cgatatcttc atgggtgcgc cgatgctggc caatagtcag gtcggcttct
     9601 tctatccgct caactggccg atctggctgt tcctgcccac gccctatgcc gttagtgcgt
     9661 cgatcctcat ccatttgtcc atcgccgggc tgggtgctta tctggccggg cggcgctcga
     9721 tgggtctggg tcgggcggcg gctctcttcg cggcggcgct gttcgctctc ggtggttatc
     9781 tgaccgcgca ggtggagcat atcaatcagt tgcaagggct ggcctggctc ccatggtatt
     9841 tcgccgtcct aacccctgtc gtgacgggcg atggcgcgcg tcaatggcgc acgttgatgc
     9901 gtggcgcgct gggtgtcggc attttgtttg ccctgcaact actggccggg cacacccaga
     9961 cggcatttat aagcggagta gccatttccc tctgggcgtt gtttcattgg ctgactgatg
    10021 cgcgcgcggc gggacgcggc attggcgtgt tggcgcgcgc aggattggtc attggcgcgg
    10081 gggtggcttt ggcggcgctg ttggcggccg tccagttgct ccccacgctc gaattggccg
    10141 ggcagtcgag caggcagggc ggcctctccc tgaacgaggt gctgtcgttt tcgtggcatc
    10201 cgttgcatat gacccgcgcg ttgctgccgg cctatggggc ggcgctcttc tctgagtacg
    10261 tcgccttttt gcccctgacg gccctcgcgc tggcggtcgt cggcacgtgg ggccggcgca
    10321 agcgaccggg tgtgttgccc tgggtgttgt tggcgcttat gatgatccta cttgccctgg
    10381 gtcggttcac ccctgtttat tatctgctgg gtcgcgtgcc agggtttgat ctgttccgcg
    10441 tgccggcgcg ttggctggcg gtggctgcgc tggcggtggc cttgctggcc gggcatggct
    10501 gggagcggtt gcgccgcttt gcggagtccg gcatccccac ggatgagcgg cgcgaggaag
    10561 cgcgagaagc atttggtcgt cccctggctg tggccagaac tggcttgttg ctgctaatcg
    10621 cctgggggta tgtagccggg tgggtagctc gctgggtgcc caccggcgcg gaagcgcctt
    10681 tcgagacgcc cgcgctcctg acgctgcttg gctggttgat cgagttcgtg ttggcgagct
    10741 tgttgctgtg ggtcattctg accgcgccgt cggaacgcgc tcgccgggcg acgacagagc
    10801 tggtgatcct ggcgttggcc gtgctgtggc ttggctcgcg ctcgctgccc tacaaccaat
    10861 tgacgacgct ggaagcgtat tttgattggc ggccgccaca agcccgcctg gccgccctgg
    10921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgacggccg atggtcgcct
    11041 gcgcgaatac ttaaccgccg cgcccgatgc ccgttggctg agcctgttca acggccgcta
    11101 tcttgtcacc gacaaaacgg gcgatctctg gcgcgaaggc gtcttctttg atcggcagca
    11161 cccaacccgc atcggggccg agcctgtgtt gcttgccgat gtcccgacct atgaggcgac
    11221 ggaactatgg atgctcgccg aggcgccgcc ggaggttgag gtcatcactg ctgaggggga
    11281 gagctggcgg ctaacgccgg agctatggca ggcgcctgat ctttatcgct tggactatcc
    11341 acgggcggcg acggccgagc gcgtcacctt gctgccatgc cccgagcaag cggctgagtg
    11401 cggcgtgaat gccctgacgc tggttgacgc gcgggacgag acctttctgc ccctgaccat
    11461 gcctccctac aggctgatct tctccggcga cgtgaagatc tatgagaacc tcgacgcgct
    11521 gccgcgggcc tttctggtgc atgagtggca ggtggcgagt gatacggtgg cagccgtcgc
    11581 gatgatgcgc gcgggtgact tcgagccggg cctgcgcgcg gtggtgaccg gcgagaacgt
    11641 accttcgtca tcggcaacgg cagggcgcgg ttccgcgacc atcgtcgatt accgaccgga
    11701 ggttgtgact ctgcacactg aggcagacgc cgaaagcttt ctgattctga ctgatgccat
    11761 ctatcccggt tgggaagcgg cgattgatgg cgtaccggtt tcggtttggt cggttgacgg
    11821 tctgtttcga ggcgtatttg tgcctgccgg ggatcatgag atcgtgatgt ccttccggcc
    11881 ggatagctat cgacgggggc tgggcctgtc agccgcgggg atactcatgc tcatcgttct
    11941 ggggctattg ctctatcaga accgcgagcg cacaatcccg gcctcaacta tttagcctgg
    12001 ttcagacacg ttagagggcg ccataaggtt agacgttcgt caagagatgt gcattcaact
    12061 gcttgattgg ttatccttgt tgattgtcag cgaacaggag aatccgtaaa ggtagtc
//
LOCUS       DAOMQG010000067        11964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2310_length_11964_cov_9.805861, whole genome shotgun sequence.
ACCESSION   DAOMQG010000067 DAOMQG010000000
VERSION     DAOMQG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_11964_cov_9.805861"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1471)
                     /locus_tag="PLC06_05690"
     CDS             complement(281..1471)
                     /locus_tag="PLC06_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRO89972.1"
                     /translation="MEDEIDLRPYIHSLIRYWRQIALCALIGAGLALFYGVSRQDSYP
                     ATTIVAFLEPTQAVQFDPRFESVTSKTTILKSLPALSMSDEVMGALLLRLDDTGIDTV
                     TDLKKRVSAESGSDLNLLYLRARTLDPVLSARLVNEWADVFVTMANDIYANRGSGQVE
                     FYTVQLQEAAQRLTATDQALIDFQAINTLPQVTNELDSLVALQVRYVELDNSLTMLES
                     DVQAARAQLTSLPTGAGGVAGEYTLLALQSRVFQLQEQLPITIQLAAPTGAASLDTGQ
                     FLSSLDAVIAEMRPRIAEQLVSLGPRILELQREKESLTARSVLLSSNRDLAMETYKTL
                     ARKLDEERLTTNDITTGFRQVSRAAVPEMPITVNLVLITLAGAAFGMILSGLAAILYT
                     WWRR"
     gene            complement(1630..2055)
                     /locus_tag="PLC06_05695"
     CDS             complement(1630..2055)
                     /locus_tag="PLC06_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89973.1"
                     /translation="MSIYASDSQLYSCFQALFGIIEAHDPKAADALLKSSLAITFNCI
                     NPTASITIDARRAPLQLAYGQTGVVPTIEVGLTADVLHCLLLGEVRLTKAIGSDLINL
                     KGPVWKTLSLADIFHYAQEFYPGVLTNHGLPTTCPRLSA"
     gene            complement(2080..2898)
                     /locus_tag="PLC06_05700"
     CDS             complement(2080..2898)
                     /locus_tag="PLC06_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO89974.1"
                     /translation="MMVNQSLLSDNDKAALEIIAATQQNIDGQRAGALLAIAAGETQA
                     AAAATHGLTEGQVSYALRKFREQGMEAFSPASRVGTAASVSPEAEVESLRAMVDQLNT
                     RVAELQQQADTRSATGEAMYTPTHLLAMVRENVAKLTPEMQLDVLRNFQGMSAEDLLD
                     LETWKGLAYMMTYSARFQADQMRGKVSDTINHVVPEPIQPGRLWQMGKSGLDRITPEF
                     AKQILSTFQGASREDLLDPNTWKGVWYMITYSLQFQAEQLKQRLTAAEEGQSPE"
     gene            3196..3576
                     /locus_tag="PLC06_05705"
     CDS             3196..3576
                     /locus_tag="PLC06_05705"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRO89975.1"
                     /translation="MTDQIEFTEEQTETSGTFLSGLRRVLMAGIGAVVLTQEQIEDFV
                     SKLVERGEIADGDARKLVTDVLDRRKSILQGGTKKAEEEYEKRIEGLLSRMNIPTKNE
                     IDSLSDTIANLNDKVDELNRRRAG"
     gene            3762..4334
                     /locus_tag="PLC06_05710"
     CDS             3762..4334
                     /locus_tag="PLC06_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRO89976.1"
                     /translation="MPVIKRYPNRKLYDTDAKRYITLNEIAALIRAGEEVAVMDHATD
                     EDLTAVVLTQIIFEQEKTQKGFLPKSVLTNLVRAGGDTLGTLRRGLTLPLDLWRHADE
                     EIDRRVQALIAKGDLAREEGQRLRDKLLSPMFTAPDAAALDDEELQRILENHDVPTRQ
                     ELERLHTQIEALSTKLESVLAHVDDGESPS"
     gene            4384..5001
                     /locus_tag="PLC06_05715"
     CDS             4384..5001
                     /locus_tag="PLC06_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89977.1"
                     /translation="MITLNGTIQRKFYFPADPLTTLIYFSDLSRVTHLLPHIVVVEAY
                     AGDKVRIRYQTVELGAYTINIFCDLDSVVNLDKMEITIGPLEDFEQVTQEVTLNSTTG
                     YGHYASTAKLSPEDDGTAIDYYFHFKSELLRPRGLRMMPKRVVDRIAHSISLGRVEEI
                     AEGFMENALDAYYDWYAEQPRDLVQQWLSTREGDLVAAERDRKTL"
     gene            5013..5717
                     /locus_tag="PLC06_05720"
     CDS             5013..5717
                     /locus_tag="PLC06_05720"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRO89978.1"
                     /translation="MRIEGHQTYAADREMLWSLLHDPNALARVLPGCESLETRGPHGF
                     WGMFVVRMGQTMETFSGVLSLERSVPLESWEFTARGNNLDGAIACRGHVLLSDEGPGL
                     TTLTYVTDIEVSGRPAQLTQRMLQTTARSFARRSLEELQQQIDARTRGYTPAVKSDGV
                     TRGASSPLALERLVLRRRLILIALVLLAALFFWRRAGDRRERLVAGQLAELLDGAGLA
                     SSVPAEPSPALSRVAA"
     assembly_gap    5798..5897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5898..6256
                     /locus_tag="PLC06_05725"
     CDS             <5898..6256
                     /locus_tag="PLC06_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRO89979.1"
                     /translation="ETRALLERVVSPMLDAGVDTLVLGCTHYPFIRPLLERIIGEDIA
                     VIDPAPAVARHTRFVLQQHRLIAPDGQEGALRCFTSGDPRQMEAFAAPVLETSCVAEA
                     VQWHDNEILTNAPDIS"
     gene            6270..6698
                     /locus_tag="PLC06_05730"
     CDS             6270..6698
                     /locus_tag="PLC06_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRO89980.1"
                     /translation="MTRRPNSARWRAIEARLSRESNIWMATVRGDGRPHLVPIWYIWL
                     EGKLYICTGRETQKYANLINNQNISLALPDALNVVVIEGEAHTADRKLIDPLAEHFYH
                     KYEWDFRYDETADWRLIEITPFKVLAWGDGYDSQEGTRVL"
     gene            6926..9418
                     /locus_tag="PLC06_05735"
     CDS             6926..9418
                     /locus_tag="PLC06_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO89981.1"
                     /translation="MRFDRFTERAQDAATRAYEIVRRYGHTQVDTEHVFLALLEQQDG
                     AVPQILVALNVDSATIIDRLDAELRASPKVSVYGGGVGQVFYTPRIRTVLELSQGEAN
                     RLKDEYISTEHIFLAILSERNTASSRILQEQGITRERVLEAVKEIRGGQRVTNRQADS
                     RYRTLEKYSRDLTQMARDGRLDPVIGRDAEILRVIQVLSRRTKNNPVLIGEAGVGKTA
                     IVEGLAQKIIVSDVPENLLNKRVVALDLGAMIAGSRFRGEFEERLKAAMEEIQRAQGE
                     IILFIDELHTVVGAGSAQGAMDASNMLKPALARGELQCVGATTLDEYRQYIERDAALE
                     RRFAPVFVDEPSAEETIQMLQGLRERYETHHSVKFSDDAIVAAVKLSSRYVTDRRLPD
                     KAIDLMDEAAAKLRVALHTLPPELKVLKTEIDKLLAGEEEASAARDYEKAQISKADRL
                     RLETEFNELREKWQSEQRLDEIVDEQDVAEVISAWTGIPISQMLETETIKLLQMEDRL
                     HERIVGQERAIAALSDAIRRSRSGMSDPRRPIGSFIFLGSSGVGKTELAKALAEFLFD
                     DEDALIRVDMSEYREQHTVSRLFGAPPGYVGYEQGGQLTEQIRRRPYSVVLFDEIEKA
                     HPDVWNSLLQLLDDGRLTDGQGRVVNFRNAVIVMTSNVGTSFVKKSGALGFAGMASAE
                     KEDHKRIEEALKQTFRPEFINRIDEIIIFEPLSEENVVEIVKLQVKDVQKRLSEHGNL
                     SIELTEPAERWLAQQGMDKDFGARPLKRAIQRYVESALSMRLLKGEFKYGDRIRIDVE
                     GDALIFTLLESGPLPIESVEAMIDSMPETLEP"
     gene            complement(9532..9963)
                     /gene="arfB"
                     /locus_tag="PLC06_05740"
     CDS             complement(9532..9963)
                     /gene="arfB"
                     /locus_tag="PLC06_05740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRO89982.1"
                     /translation="MDTDLVIINESLAVPESELQFRFSTGSGPGGQHVNKTATRVTLL
                     FDVASSPSLDEETRVRLLDRLASRLDRRGLLHIDVQESRSQWQNRMTAVARFQKMMAE
                     ALVEQPERRPTRPTRRSREDRLEEKRRRSDVKRDRRQRWDD"
     gene            9994..10968
                     /locus_tag="PLC06_05745"
     CDS             9994..10968
                     /locus_tag="PLC06_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89983.1"
                     /translation="MATLPSQPFVHILFLDGVGLAQPGAADNPFVVAAMPQLTGLLGE
                     GWYLAGRERITTPRASLVPTDANLGVDGRPQSATGQATILTGRNVPLLVGEHYGPKPN
                     RAVADVIRAGTLFQETVAAGGRAALITPYPERYFAAIESGRRLLSSVPLAATEAGLTL
                     MGADDLRAGRAISPDFTGAGWREQLGYADMPTLSLAEAGRRIAAVAAEYEFSFFEHWP
                     TDQAGHRGTLAEAVAHLEAIDAVMGGLLEAWDDTRGLLILTSDHGNLEEKGHRQHTRN
                     PVPTILLGRDQARYAAAIHDLADIAAVVRDCLGLPVPAYAGYIDSTQS"
     gene            10983..11477
                     /locus_tag="PLC06_05750"
     CDS             10983..11477
                     /locus_tag="PLC06_05750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRO89984.1"
                     /translation="MPISSEDFRDVLRHFPSGVTVVTIKPYGSDEVHGLTVSAFASIS
                     PEPPLILISIDHRAHAYRIMETAGTTFAVNILSHDQMEISNRFAWLKDEDRFAEGDWG
                     TAVTGAPVLNDALAWLDCTIYSRFAAGSHTIYVGEVQASSVTRPDVKPLVYWNRGYRK
                     LVDK"
     gene            complement(11486..>11964)
                     /locus_tag="PLC06_05755"
     CDS             complement(11486..>11964)
                     /locus_tag="PLC06_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRO89985.1"
                     /translation="SQRRKTVGARLEDGVLVIRAPASISDKELAPIIEGLRKRMVRRA
                     RVVPQTDEMLEKRARELNRKYFGGRLKWQSVRYVKNQNSRFGSCTPADGTIRLSDRLA
                     TMPTWVRDYVLVHELAHLLEANHGPNFWKLVNRYPLTERARGYLMAVGLGPDAGAE"
BASE COUNT         2269 a         3487 c         3737 g         2371 t
ORIGIN      
        1 cgccaggcga tctgttcctc gggtgaaaga gttaaaactc ccgtcaactc gtgagccgag
       61 tcttcaagag ccacactgtc gccataagcc aaggctgtat tcaggcggat attggcgcca
      121 ttctggtatg cgagagaaat aatcctcgcc ccgaacgcca cgatgagcga agccaccagg
      181 atagctccgc ctatcagacg aactgtatgg gtcgaggtgt aaggattgga tctcatgccg
      241 acggaacccg ggcggtgcca tggccgctcg cctgtaagga ctatcgccgc caccaggtat
      301 agaggatggc cgccaagccg gataatatca tcccaaacgc cgccccagcc agggtaatca
      361 gcacaaggtt aaccgttatc ggcatttcag ggacggcggc gcggctcacc tgacgaaagc
      421 cggtggtgat gtcattggtg gtcagacgct cctcgtccag cttgcgggcg agcgtcttgt
      481 acgtctccat cgccagatcg cgattggagc tgagcaacac ggatcgagcg gtcagagatt
      541 ccttctcgcg ttgaagttcc agaattcgcg gccccaatga caccagctgc tcggcgatcc
      601 tcgggcgcat ctcggcgatc acggcgtcga gtgaactaag gaattggccc gtatccagcg
      661 acgcggcccc tgtgggggcg gccaactgga tggtgatcgg cagttgctct tgcaactgga
      721 aaacccgcga ctggagcgcc agtaacgtat attcgcccgc cacgcctccc gcaccggtgg
      781 gcagactggt tagttgcgcg cgcgccgcct ggacgtcgct ctccagcatg gtaagtgaat
      841 tatcgagttc cacatagcga acctgcagtg cgaccagcga gtcgagttca tttgtaacct
      901 ggggaagggt gttgatcgcc tgaaagtcga ttaaagcctg atcggtagcg gtcaatcttt
      961 gcgccgcttc ctgcagttgc acggtgtaga actcgacctg gccactaccg cgattggcat
     1021 atatgtcgtt ggccatcgtc acgaacacgt cggcccactc attgaccagt ctggcgctca
     1081 gtacgggatc tagagtgcgg gcgcgcaagt agagcaggtt gagatcgctg cctgattcgg
     1141 ctgaaacgcg cttcttgaga tcagtgaccg tgtcgattcc tgtgtcgtcc agccggagca
     1201 acaacgcgcc catcacctca tcgctcatgc tcagcgcggg caaggatttg aggatcgtgg
     1261 tcttgctggt gacggattcg aagcggggat cgaattgcac ggcctgggtc ggctccagga
     1321 aggccacgat ggtggtagcc gggtagctgt cctgccggct caccccgtaa aagagagcca
     1381 ggcctgcgcc aataagcgcg catagcgcga tctgccgcca gtaacgaatc aggctatgga
     1441 tgtaaggtcg aagatcgatt tcatcttcca agtgttactc tcccaaaata acaggtcgga
     1501 gttttccgtc ctcatgaatc aataattcct cgcgcgcgga aaggagtggc ttgggcatcg
     1561 aaaggcttcc ggttggatag cagggtccgg atagtatatc gtatgatatg ctgtcgtatt
     1621 gtgccggtgt caggccgaaa gcctcgggca cgtggttggc aggccgtgat ttgtcaatac
     1681 ccccggataa aactcctgcg cgtaatgaaa gatatcggcc agcgacagcg tcttccacac
     1741 cgggcctttc agattgatca ggtcgctgcc gatggctttc gtcaggcgaa cctcgcccag
     1801 caacaggcaa tgcagcacat cggctgtcag gcctacctcg atcgtcggca ctacgccggt
     1861 ttgcccgtag gccagctgca gcggcgcgcg gcgggcgtcg atggtgatcg acgctgtcgg
     1921 gttgatacag ttgaaggtga tcgccagcga cgacttcagc aaggcgtcgg ctgcctttgg
     1981 gtcatgggct tcgataatgc cgaacagggc ctggaagcat gagtacagtt gagagtcgct
     2041 ggcgtagata gacattggcg tgcatcctgc gttgaggggt tattccggcg attgtccttc
     2101 ctcggcggcg gtcaggcgtt gtttcagttg ctcagcctga aactggagcg agtaggtgat
     2161 catataccac acgcccttcc aggtgttggg gtccagcagg tcttcgcgcg atgctccctg
     2221 aaacgtgctc aatatctgct tggcaaactc gggcgtaatg cgatccaggc cggatttgcc
     2281 catctgccag agacggccgg gctggatggg ttccggcacg acgtggttga tcgtgtcgct
     2341 caccttgccg cgcatctgat cggcctggaa gcgggcggag taggtcatca tataggccag
     2401 ccctttccag gtctccaggt cgagcaagtc ctcggccgac atcccctgga aattgcgcag
     2461 cacgtcgagc tgcatctccg gggtgagctt ggccacattc tcgcgcacca tggccagcaa
     2521 gtgggtcggg gtgtacatcg cttcgccggt ggcggacctg gtgtcggcct gctgttgcag
     2581 ctcggcgacc cgcgtgttga gctggtcaac cattgcccgt agcgattcga cctcggcttc
     2641 cgggctgacg gatgcggccg ttccgactcg tgatgccgga gagaaggctt ccatcccctg
     2701 ttcgcggaac ttgcgcagcg catagctgac ctgtccctcg gtgaggccgt gggtggctgc
     2761 ggcggcggcc tgcgtctcgc ccgccgcaat ggccagcagc gcgccagcgc gttgcccgtc
     2821 tatgttttgt tgcgtggccg caattatttc gagagccgcc ttatcgttat cggacaggag
     2881 cgattggttg accatcatgg ttcctcccaa ggttattcga tatctgacag aatatctctc
     2941 cattataact aaagaacgcg taatgcgtca cttttgatcg agcaagaccc tgtcctgggg
     3001 ccttcacctg atgatcatct gaccgtgtta aattgcggtg gatatgatcg cccctgcttt
     3061 gtttgacagc gcgactgcgc atatgatact attccttata atgcgctgcg tcatgatggg
     3121 agagcgctgt atacatttca taggcttata ttcccattgt tatcctgaat taccggcccc
     3181 ggaggagtgc tttcaatgac cgatcagatc gaattcacgg aggaacagac cgaaacgtcc
     3241 ggcacgttcc tttccggcct tcgccgggtc ctgatggccg ggataggcgc tgtggttctg
     3301 acccaggaac agattgaaga tttcgtgagc aagctggtgg agcgcggcga aatcgccgat
     3361 ggcgacgctc gcaagctcgt gaccgatgtg ctggatcgcc gtaagagcat cctgcagggc
     3421 ggcacgaaga aggccgaaga agaatacgag aagcggatcg aaggcctcct gtcgcgaatg
     3481 aacatcccca ccaagaacga gatcgattcc ctgagcgata cgatcgccaa cctgaacgat
     3541 aaggtcgatg agctgaaccg tcggcgggcc ggatagatct cgctcctgtt cgtgttccat
     3601 ccgcggccgg ctccctgatt ttcggtggcc cgttgattcg tcgaggggtg ggtgccatcg
     3661 ggagtcgccc tgggaagatg gtgctcgcgt tggcagtcat gccattacag cttatacttc
     3721 ccatcgacaa tcgtccatgg gacactattc ggaccaatca catgcccgtt atcaagagat
     3781 atccgaaccg caagctatac gacaccgacg ccaagcgtta catcacgctg aacgagatcg
     3841 ccgcgctcat ccgtgccgga gaggaagtcg cggtgatgga tcatgcgacc gacgaagacc
     3901 tgacggccgt ggtgctgacc caaatcattt ttgagcagga gaagacccaa aagggctttc
     3961 tgcccaaatc tgtgctcacc aatctggtgc gggccggagg cgatacgttg ggaacgttgc
     4021 gccgggggtt gacgctgcct ctcgatctgt ggcggcatgc ggacgaggag atcgatcggc
     4081 gcgtgcaggc gctcatcgcg aaaggggatc tggcgcggga agagggccaa cgactgcgcg
     4141 acaagctcct gtcgcccatg ttcaccgctc ccgacgccgc tgcgctggat gacgaagagt
     4201 tgcaacgcat cctcgagaac cacgatgtcc cgacccgcca ggaactggag cgactgcaca
     4261 cgcagataga ggcgctctcg accaagctgg agtccgtcct ggcgcatgtg gatgatgggg
     4321 agtcgccgtc ctgatccgcg gtcgagcgtt ctcttttttg ttgaatcact cactatttgc
     4381 cagatgatca ccctgaacgg caccatccag cgcaagttct atttcccggc tgatcccctg
     4441 acgaccctca tctatttcag cgatctcagt cgggtcactc atttgttgcc ccatatcgtg
     4501 gtagtcgaag cttatgccgg cgacaaggta cggattcgct accagacggt cgaattgggg
     4561 gcgtatacta tcaatatctt ctgcgatctc gatagcgtgg ttaacctgga taaaatggag
     4621 atcaccattg gcccgttaga ggatttcgag caggtgacgc aggaagtgac gctgaactcg
     4681 acgacaggct acggtcatta tgccagcacg gccaagttat cgcccgaaga tgacggtacg
     4741 gccatcgatt attatttcca tttcaagtcg gaactgctcc gtccgcgggg gctgcgcatg
     4801 atgcccaagc gcgttgtcga tcggatcgcg catagcatct cgctcggccg cgtcgaggaa
     4861 atcgctgaag gattcatgga gaacgcgctg gatgcctact acgactggta tgccgagcag
     4921 ccgcgcgatt tggtgcagca atggctgtcc acgcgcgaag gcgatcttgt ggccgcggag
     4981 cgcgaccgaa agacgctata gaggttgccg tcatgagaat tgagggacac cagacctatg
     5041 ccgccgaccg tgagatgttg tggtcgctgc tccacgaccc gaacgcgctt gctcgcgtgt
     5101 tgcctggctg tgaatcgctg gaaacacgcg ggccgcacgg gttttggggg atgtttgtcg
     5161 tgcgcatggg ccagacgatg gagacattct ccggcgtcct gtcgttggag cggtctgttc
     5221 cgctggagtc ctgggagttc accgccaggg ggaataacct ggacggggca atcgcctgtc
     5281 gcggccatgt gcttttgagc gatgaagggc cgggcctcac aaccctgacg tatgtgaccg
     5341 acatcgaggt ttccggccgc cctgcccaat tgacccagcg catgctccag actacggccc
     5401 gctctttcgc acgtcgcagc ctcgaggagc tgcagcagca gatcgacgct cgcacgcgcg
     5461 gctatacccc tgccgtgaag agcgacgggg tcacccgcgg cgcatcttcg cctttggcgc
     5521 tggagcggct cgtcttgcgg cggcgcctaa tcctcattgc tctcgtgttg ctggcggcgc
     5581 tgttcttttg gcgtcgggca ggcgatcggc gggagcgcct cgttgccggg cagctcgccg
     5641 aactgctcga tggggccggt ctcgcttcgt ctgttcccgc tgaaccgtca cccgccctgt
     5701 cgcgggttgc agcctgaagc cggccgtgag cgatccccgg caaccctcaa cgactgacca
     5761 aacggccgcc gggccgcttg gcgtgctcga ttcaggcnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnncgg aaacgcgcgc gctcctggag cgtgtggtct cgccgatgct
     5941 ggatgcgggc gtcgatacgc tggtgctggg ctgcacccac tacccattta tcaggccgtt
     6001 gctggaaagg atcatcggcg aggacattgc cgtcatcgat ccggctccgg cggttgcccg
     6061 tcacacgcga tttgtgctac aacaacaccg attgatcgcc cccgacgggc aggaaggcgc
     6121 gctgcgatgc ttcaccagcg gcgatccgcg acagatggaa gcgtttgcgg cccccgttct
     6181 ggagacctca tgcgtggccg aagccgttca gtggcatgac aacgagattc tgactaacgc
     6241 gccggacata tcctaatcga atgcggccca tgaccagacg accgaactcc gcccgctggc
     6301 gcgccataga agcccgattg agccgggaga gtaatatctg gatggcgacg gttcgcggcg
     6361 acggccgccc ccatctcgtg ccgatctggt atatctggct tgagggcaaa ctctatattt
     6421 gcaccggtcg cgagacgcaa aaatacgcca atctgatcaa taaccagaat atttcgctgg
     6481 ccttgccgga tgcgctcaat gtggtggtta tcgaaggtga agcgcacacg gccgaccgca
     6541 agctcatcga cccgctggcc gaacatttct accataaata cgagtgggat ttccgttacg
     6601 acgagacagc cgactggcgg cttatcgaga tcacgccgtt caaggtattg gcctggggcg
     6661 acggttacga ctcgcaagag gggacgcgcg tattgtagac agaaagaagc gccgtcgatt
     6721 atgggtggtc agggtctaac gtgaatccaa ttatcaggcg ctatacttcc atcattggcg
     6781 ggggattctt tgcgaatgat cccctgcaaa gtttctgaat agaaccatac tttatcggta
     6841 tatcattcat ggatgatttg gatggccacg aacgccggcg atagcgggtg ggttttgcgg
     6901 tcacctcatc aaggacaata gatagatgcg attcgatcgt ttcacggaac gtgcccagga
     6961 cgccgccacg cgcgcttatg agatcgtgcg ccgctatggc cacactcagg tcgataccga
     7021 gcacgtcttt ctggcgttgc tggagcagca ggatggggcc gttccccaga ttctggtcgc
     7081 tctcaacgtc gacagtgcaa caattatcga ccgcctggac gccgaactgc gggccagccc
     7141 caaggtgtcg gtctatggcg gtggcgtggg gcaagtcttc tatacgcccc gcatccgcac
     7201 tgtcctggag ctttcccagg gcgaggccaa ccggctgaag gatgagtaca tttccaccga
     7261 gcatatcttc ctggccattc tgagcgagcg caacacggct tcatcgagga tcctgcagga
     7321 acaaggcatc acgcgcgagc gcgtcctcga agcagtcaag gagatacgcg gcggccagcg
     7381 cgtaaccaat cggcaggccg acagccgcta tcggacgctg gagaaataca gccgcgacct
     7441 gacgcagatg gcccgcgacg gccgtctcga cccggtcatc ggccgcgacg ccgaaatcct
     7501 gcgcgtcatc caggttctca gccgtcgcac caagaacaac cccgtgctca tcggcgaggc
     7561 gggcgtcggc aagacggcca tcgtcgaggg gctggcgcag aagatcatcg tcagcgatgt
     7621 gcccgaaaac ctgttgaaca agcgcgtcgt ggccctcgac ctgggcgcga tgatcgccgg
     7681 cagccgcttt cgcggcgagt ttgaggagcg cctgaaggcg gccatggaag agattcagcg
     7741 tgcccagggg gagatcatcc tcttcatcga cgaactccat accgtcgtgg gggcgggttc
     7801 ggcccagggc gcgatggacg ccagcaatat gctgaagccg gcgttggcgc gcggcgagtt
     7861 gcaatgcgtc ggcgcgacca cactggacga gtatcgtcaa tacatcgagc gcgacgccgc
     7921 gctggagcgc cgttttgcgc cggtgttcgt cgacgagccg tcggccgagg agaccatcca
     7981 gatgctccag ggcctgcgcg agcggtatga aacgcaccac agcgtcaagt tctccgacga
     8041 cgccatcgtg gccgccgtca agctctcgtc gcgctatgtg accgatcgcc gcctgcccga
     8101 caaagccatc gatctgatgg acgaagcggc ggccaagctg cgcgtcgctc tccacacctt
     8161 gccgcccgaa ctgaaggtgc tcaagacgga gatcgacaag ctcctggccg gcgaggaaga
     8221 ggccagcgcc gcgcgtgatt acgaaaaggc gcagatcagc aaggctgacc ggctgcgtct
     8281 ggaaaccgag ttcaacgaat tgcgcgagaa atggcaaagc gagcaacggc tcgacgagat
     8341 cgtcgatgag caggacgtgg ccgaggtgat ctccgcctgg acgggtatcc ccatctccca
     8401 gatgctggaa accgagacga tcaagctgct gcaaatggaa gatcggctgc acgagcgcat
     8461 tgtgggccag gaacgggcca tcgccgcgct gtcggacgct attcgccgca gtcgttcggg
     8521 catgagcgac ccgcgccggc ccatcggttc gtttatcttt ctgggtagtt ccggcgtggg
     8581 caagacggag ctggccaagg cgctggccga gttcctgttc gacgacgaag acgctttgat
     8641 ccgcgtggat atgagcgaat atcgcgagca acataccgtc agccggctgt ttggcgcgcc
     8701 tccgggctat gtaggctatg agcagggcgg ccaattgacc gagcaaatcc gccgccggcc
     8761 ctattctgtc gtgctgttcg acgagatcga gaaggcccac cccgacgtgt ggaactcgct
     8821 gttgcaactg ctcgacgatg gccgcctgac cgacgggcag ggacgcgtgg tcaacttccg
     8881 caatgcggtc atcgttatga ccagcaatgt gggcacatcg ttcgtcaaga aatcgggcgc
     8941 gctgggcttt gcgggcatgg cctcggccga gaaggaagat cacaagcgca tcgaagaagc
     9001 gctgaagcaa actttccggc cggagttcat caaccgcatc gacgagatca tcatcttcga
     9061 gccgctctcc gaggagaacg tcgtcgagat cgtcaagctg caggtcaagg atgtgcagaa
     9121 gcggctgtcg gaacacggca atctgagcat cgagctgacc gagccggccg agcgctggct
     9181 ggcccagcag gggatggaca aggacttcgg ggcgcggccg ctcaagcggg ccatccagcg
     9241 ctatgtggaa agcgccctgt ccatgcgctt gctgaagggc gagttcaagt atggcgaccg
     9301 catccgcatc gacgttgagg gcgacgcatt gatctttacg ttgctggaga gcgggccgct
     9361 gccgattgaa tcggtggaag cgatgattga ctccatgccg gagacgctgg aaccgtaatc
     9421 cggttggtgg tgaatgtgac ggcctgatgg gttggcgatt aatgcgccgg cctgtcaggc
     9481 cgttggcgat cggacgatcg ggcgatcgga cgatcgggcg atcgggccgg gttagtcgtc
     9541 ccaacgttgc cggcggtcgc gcttgacgtc gctgcgacgc cgtttttctt ccaggcggtc
     9601 ttcacgggat cggcgcgtcg gccgcgtggg gcggcgctcc ggctgctcga ccagcgcttc
     9661 ggccatcatt ttctggaagc gggcgacggc ggtcatgcgg ttctgccatt ggctgcgcga
     9721 ctcctgcacg tcgatatgga gcagcccgcg ccggtcgagc cgcgacgcca gccgatccag
     9781 cagtcggacg cgggtctcct cgtcgaggct gggcgaactg gccacgtcga acagaagggt
     9841 cacacgcgtg gccgttttgt tcacgtgttg gccgcccggc ccgctgccgg tggagaagcg
     9901 aaattgcagc tccgactccg gcacggccag agattcgttg atgatgacca gatcggtgtc
     9961 catatgttgg aattatcgga gagtcgttag cttatggcaa cgttgcccag tcaacccttt
    10021 gttcatatcc tgtttctgga tggcgtcgga ttggcccagc cgggcgcggc cgataacccg
    10081 tttgtggtgg cagccatgcc gcagctgaca ggcttgctgg gcgaggggtg gtatctggcc
    10141 gggcgcgagc gcatcaccac gccgcgcgcg tcgctggtgc cgacggatgc caacctcggc
    10201 gtggacggcc gcccgcagag cgccaccggc caggcgacta tcctgaccgg ccgcaacgtg
    10261 ccgctgcttg tcggcgaaca ttacggcccc aagcccaacc gggccgtcgc cgacgtcatt
    10321 cgcgccggga cgctgttcca ggagacggtg gccgccggcg gccgggcggc gctcattacg
    10381 ccctatcccg aaaggtattt cgcggctatc gagagcgggc ggcgcttgct gtcgtcggtg
    10441 ccgttggcgg ctaccgaagc ggggctgacg ctcatgggcg cggatgatct gcgggccggt
    10501 cgcgccatca gccccgactt caccggcgcg ggctggcgcg aacagctcgg ttatgccgac
    10561 atgccgacgc tctccctggc ggaagcgggg cgacgcattg ccgccgtggc cgcggaatat
    10621 gagttttcgt tcttcgagca ttggccgacc gaccaggccg ggcatcgggg cacgttggcc
    10681 gaggcggtcg cgcatctgga agccatcgat gctgtgatgg gcggcttgct ggaagcctgg
    10741 gacgacacgc gcggcctgct gattctgacc agcgaccacg gcaatctgga agagaagggg
    10801 catcgccagc acacccgcaa cccggtgccg acgatcctgc tcggccgcga ccaggctcgc
    10861 tatgcggcgg ctatccacga tctggccgac atcgccgccg tcgtgcgcga ttgcctgggg
    10921 ctgcccgttc ccgcctatgc gggttatatt gactcgactc aatcctaatc aaggaatttt
    10981 ttatgccgat ttcatctgaa gattttcgcg atgttttgcg tcatttccct tcgggcgtga
    11041 cggtggtcac gatcaagccc tatggctccg atgaagttca cgggctgacc gtttccgctt
    11101 ttgcatcgat ctcgcccgag ccaccgctca tccttatctc aatcgatcac cgcgcccacg
    11161 cctaccggat tatggagacg gccggcacga ccttcgccgt caatatcctg agccatgacc
    11221 agatggaaat atcgaatcgt tttgcctggc tgaaggatga ggatcgtttt gccgaggggg
    11281 attggggcac ggccgtcacc ggcgcgccgg ttctgaatga tgccctggcg tggctcgatt
    11341 gcaccatcta ctcgcgcttt gccgcgggca gccacacgat ctacgtcggc gaggtgcagg
    11401 ccagcagtgt cacgcggccg gatgtgaagc cgctcgtcta ctggaatcgg ggttatcgca
    11461 agctggtgga caagtagccc ccggcctact ccgcgccggc gtcgggtccg aggcccacag
    11521 ccatcagata gccgcgagcg cgctcagtga gggggtaacg gttgacgagc ttccagaagt
    11581 tcggcccgtg attggcttcc agaagatggg ccagttcgtg gacaaggacg tagtcgcgca
    11641 cccaggtggg catggtcgcc agccggtcgc tcaggcgaat ggtgccgtca gcgggcgtgc
    11701 agctgccgaa gcggctgttc tggttcttga catagcgcac ggattgccat ttgaggcggc
    11761 cgccaaagta cttcctgttc agctctcggg cgcgcttctc cagcatctcg tccgtttgcg
    11821 gaaccacgcg ggcgcggcgg accatgcgct ttcgcaggcc ctcgatgatg ggggccagtt
    11881 ccttgtcact gattgacgcc ggggcgcgaa tgacgagcac gccgtcttcc agccgcgcgc
    11941 cgacggtctt gcgccgttgg ctgc
//
LOCUS       DAOMQG010000068        11954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2312_length_11954_cov_13.170182, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000068 DAOMQG010000000
VERSION     DAOMQG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_11954_cov_13.170182"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1468
                     /locus_tag="PLC06_05760"
     CDS             179..1468
                     /locus_tag="PLC06_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89986.1"
                     /translation="MIFLLPLAACDQLQVGPGAVIPPTTVATVAPTAAAPQITVESPG
                     AVEPVEQIATLRLWLPPEIGARSESGTQELSSQVRAFETGHGDLDVIIEQKPVDGAGG
                     IINYLQSGRKIAPDIMPDLVAVPSSLLSDTRVNGLFYPMSSVTGEMIGEQIYPASAAQ
                     VTVSGQPLGYPFAIMGLTHLIYDPKVITQTVPIQWTEFISDTNHTLVLPADSREGALL
                     GLQFYMAEGGALVDSTGRPLLEPEPLARALATIGVRKENLLQSHQLKTLDEAWQYHQL
                     GLSDFMWMRSEFLLGRQALDPSLLNSQSYMAVPGLNGHLLPLVTSWSWAITTEDPARQ
                     KLAEELIVNLTLPENLANWGQRSQVLAAQRAAMEIMSEQNPYLQFASGQLELARAMPV
                     NETSRLMDVLGDAVFQLLTTDTPPEIIAEQAVATLRQ"
     gene            complement(1483..2766)
                     /locus_tag="PLC06_05765"
     CDS             complement(1483..2766)
                     /locus_tag="PLC06_05765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO89987.1"
                     /translation="MISMHTCPLAMLGGKKTGGMNVYVRDLSRELGSMGIHVDVFTRS
                     QDDCVPMVNHELGINARVIHIPAGPEAPIPVSEVGNYIDEFAAGIANFATTENLHYDI
                     IHSHYWLSGLVAEKLEHYWGTTPIVHMFHTLGHMKNRIATDAAERAPQERIDGESHVL
                     SIANQIIAATPAEQAQLNWLYGANMEKVIVIPPGVDLERFQPIDKAEAKKRVGIPCGD
                     KTIMFAGRIEPLKGIDTLIQAMALIHQRCPDSVANTCVAIIGGDPWADNPDAEMTRLQ
                     AMRQELGIHDIIMFLGAKDQDVLPNYYAAAEMVVMPSHYESFGMVALEAMAMGRPVIA
                     SEVGGLAFLIHDGFNGYHVPTRDPEALAAKIYELLSNDDCRAEMGRMARKSAEKFDWS
                     IIARHIVEVYLHLLDPASPKPEGILLQTMEDYPFV"
     assembly_gap    2925..3024
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3025..3410)
                     /locus_tag="PLC06_05770"
     CDS             complement(<3025..3410)
                     /locus_tag="PLC06_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO89988.1"
                     /translation="MSDSMKCYSYSGVPGFLELAHEWDQLAGQGITNTPFQRLAYQKA
                     WWSHLCPPNSELSTVATRSDKGELLGIGCFYIHDGKIYFNGSMEETDYLDLIVRDDHV
                     EQVWQATIRCLQDYQPDRRHVLDLYN"
     gene            3556..4086
                     /locus_tag="PLC06_05775"
     CDS             3556..4086
                     /locus_tag="PLC06_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HRO89989.1"
                     /translation="MSNLRFSNAKAYSGKIAMTDKTQAMRALEGKRIAYEVMTYPDDM
                     RDAEEIALYLDIEPETVFKTLVVMPPDPGKKPLLVMVPADHQLDLKKLADVAGAKKLK
                     MATHREAEQLTGLQVGGISAVALLGKPFVVFIDRSAEQQSRIIISAGKRGVQLVLAVK
                     DLIKLTNARVVDVSVV"
     gene            complement(4154..4468)
                     /locus_tag="PLC06_05780"
     CDS             complement(4154..4468)
                     /locus_tag="PLC06_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HRO89990.1"
                     /translation="MTNMIASKEEQLMESLRSVIDPEIGLNIVELGLVRNLEVDSNTD
                     AANITMILTTPFCPYGPQIIEQVRIVGNQVMPGGVKVEIGTELWDPSMMEEGAGGDWG
                     LF"
     gene            complement(4475..5173)
                     /locus_tag="PLC06_05785"
     CDS             complement(4475..5173)
                     /locus_tag="PLC06_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HRO89991.1"
                     /translation="MNMPDQNPDEFVNRKKRTTRETILHTIKQSPQSTVEELAEAADI
                     SPVTVRHHLNALLAEGTIEASSVRRKVGRPYYVYSLSEKGQELFPKRYVRLTSRLLDE
                     MKGRLPAAVIDDIFKGVVDNVLEEHYGEFEHLPLEDRLDYLVNLLSEEGFLSAWERTA
                     DGYRLVEYSCPYLSITGAHGEVCNFDRQLMRGVLQLDVIQETCMLHGANSCQFNIKIE
                     SNVTEPAVPAIEAN"
     gene            5455..6237
                     /gene="sufC"
                     /locus_tag="PLC06_05790"
     CDS             5455..6237
                     /gene="sufC"
                     /locus_tag="PLC06_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRO89992.1"
                     /translation="MTAALEINDLHVNIGENRILKGINLQVKQGEIHALMGPNGSGKS
                     TLAYTLAGHPSYEPTAGQVTFDGMDLLEMEADERSRSGIFLAFQYPVAVPGVTVAKFL
                     RQAINSRRKAENPEDTGMPIPEFRRLLRAKMDLLGIDQKFAGRYLNEGFSGGEKKRAE
                     ILQMAMLEPKIAIMDETDSGLDIDALRIVAEGAGRLHEINDMGVLVITHYQRILNYIQ
                     PNHVHIMLDGRIVESGGPELALHLEENGYDWLREKYGMAEVA"
     gene            6237..7676
                     /gene="sufB"
                     /locus_tag="PLC06_05795"
     CDS             6237..7676
                     /gene="sufB"
                     /locus_tag="PLC06_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRO89993.1"
                     /translation="MADQVLERELTENEIVAGVNEDYAAKYGFADVEDYVFKAEKGLN
                     ENVIRAMSAMKREPEWMLELRLKAYQYFLERPMPEWGADLSDIDFENIYYYIKPTERQ
                     EDSWEDVPGYIKNTFDKLGIPAAEQKFLSGVAAQYESEVVYHNIKKDLEAKGVIFLGM
                     DDGLRQYPDLVKEYFSTIIPPNDNKFAALNTAVWSGGSFIYVPPGVHIDMPLQAYFRI
                     NAKNSGQFERTLIIVDEGAYVHYVEGCTAPIYSTDSLHSAVVEIVVKKGGRCRYTTIQ
                     NWSNNVYNLVTKRAMAYENATMEWVDGNLGSKTTMKYPAVYLMGEGAHGEILSIAFAG
                     TGQHQDAGGKVVHMAPRTSSRIISKSISKGGGRASYRGLLKVAEGAYDCKSNVVCDAL
                     LLDEHSRSDTYPYIEINEQDVTIGHEASVSKVGEEQVFYLMSRGISEDEANAMIVNGF
                     IEPLVKELPMEYAIEMNRLIQLQMEGSIG"
     gene            7669..8976
                     /gene="sufD"
                     /locus_tag="PLC06_05800"
     CDS             7669..8976
                     /gene="sufD"
                     /locus_tag="PLC06_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRO89994.1"
                     /translation="MAELLPFNRDAVVQLSASLNEPEWMLDLRLKAWEVFASLPVPTT
                     REEAWRRTDIRRFKLDSFGISLNGNGATGADVPSHLAAQLTDDAAGGVLVEVNGLVKE
                     YSLSDELREQGVIFCDMHTAVREYGDLVRKYFMTEAVRADEGKFAALHAAFWRGGVFL
                     YVPPNVSAAAPLHSALWATSGRTFTHTLVVLDTNSEATFIDEYASPAKGEGQALHNGA
                     IELIVRDGASLHYASLQDFGTNVWQFNHECGRVGRDGKLDWITSVMGTRLTKAFQTSE
                     LDAPGAWARMSGIFFTNGRQHIDLDTQQNHNAADTTSDLLYKGALKGDSRSVWQGMIK
                     ALPGSQRIDGFQANRNLMLDRTARADSIPGLEIQADDVRCTHASTVGKLDQEEIFYLM
                     SRGIPRTDAIRMVIQGFFDPVMQRIPFEGVRTQIFGRILEKIG"
     gene            9100..9969
                     /locus_tag="PLC06_05805"
     CDS             9100..9969
                     /locus_tag="PLC06_05805"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRO89995.1"
                     /translation="MDQNTYAHEEVLVSTQWVADNLHRTDKIRLVESNEDVLLYSTGH
                     IPNAVHIDWVADLNDAVRRDYLNEEQFAALLSRNGIDNDTTVVFYGDKNNWWATYAFW
                     VFKLFGHEDCRIMNGGRQKWIAEGRELTREKPSFAPTDYTPAPRVDYKIRAFRDQVLA
                     HVMADQPLVDVRSPQEFSGELLHMPGSPQEGALRGGHIKGAVSVPWSQAVAEDGTFKS
                     AEALKSIYEGREGLSPDDNVIAYCRIGERSSHTWFVLTYLLGYANVRNYDGSWTEWGN
                     LVGVPIENPSRQK"
     gene            9977..11206
                     /locus_tag="PLC06_05810"
     CDS             9977..11206
                     /locus_tag="PLC06_05810"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRO89996.1"
                     /translation="MYDVDRIRADFPILGIEVYPGVPLVYLDSAASSQKPAAVIAAMD
                     EYYRQYHANVHRGVHRLSELATNAYESARVRIGTFIGAPSPDEIVFVGNTTEAFNLVA
                     YSWGRHNISAGDEILLTEMEHHANIVPWQMLAQGTGVTIRYLPVDENGRLALDKLDDY
                     LTERTRLFSFTGASNVLGTINPVRELVEAAHAVGALAMVDAAQLVPHMPIDVEALGCD
                     FLAFSSHKMCGPTGIGILYGKRELLEAMPPFMGGGDMIRRVTFDGFVPNEIPWKFEAG
                     TPRIAEAVGLGAAVDYLDSLGMEAVHAHELSITNYALESLSEIPGLTVLGPPATQRGG
                     LVSFTMQGLHPHDIAEILDKDGIAIRAGHHCAMPLHQKLGISASARASFYVHTTMDEV
                     DKLTTALHRARSVFRLD"
     gene            11250..11651
                     /locus_tag="PLC06_05815"
     CDS             11250..11651
                     /locus_tag="PLC06_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRO89997.1"
                     /translation="MDANIYREQIIDLYEHPLNYGELPTPDFTYEEDNPLCGDVIRID
                     VVLDEQKRVKEVAWRGDGCAISQAAASLLTEEIKGKTLDEVKSFTSEELLELVGVPLS
                     MARVKCALLSLKVLKAGAYGIPDPDTIRKHE"
     gene            11698..>11954
                     /locus_tag="PLC06_05820"
     CDS             11698..>11954
                     /locus_tag="PLC06_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HRO89998.1"
                     /translation="MHFDLEEESAILLNIDGKYYCIADLCTHDGGPLEDGDVLDFQIE
                     CPRHGARFDIRTGKVTRLPATDPIPVYPVSIEDGIVYVDAP"
BASE COUNT         2644 a         3155 c         3317 g         2738 t
ORIGIN      
        1 ggtggaagcc aacagatgca atttcccaat gcgtcgtgtg tatccgatga tgaaaatcat
       61 agtacagtaa attaccctga atccaacata cgggcaacta tctgcaagag tgctatactt
      121 tttccacgcc atgaataccg gataccctcc tcaatcgcgc tggttcgttt tgctcccttt
      181 gatttttctg ctacctctcg ctgcgtgtga tcaactccag gtagggccgg gcgccgttat
      241 ccccccgaca acagtcgcca ctgtcgcgcc aacggctgcg gctccgcaga ttacagtcga
      301 aagccccggc gcggttgaac cggtggagca gattgccacc ctgcggctat ggctcccgcc
      361 ggaaatcggt gcgcgatctg agtcgggaac ccaggagtta tccagtcagg tacgagcatt
      421 tgaaacaggg catggcgatc tggatgtgat tattgaacaa aagccggtcg atggtgccgg
      481 ggggatcatt aattatctcc agtcgggcag aaaaattgcc ccggatatca tgcctgatct
      541 ggtagccgtg ccatcatctt tgctcagcga taccagggtg aatgggcttt tttatcctat
      601 gagcagcgtg accggcgaga tgattggcga gcagatatat ccggcttcag cggcacaagt
      661 tacggtgagc ggccagccgt tgggctatcc tttcgcgatt atggggttga cccacctgat
      721 ttatgatccg aaggtgatta cccagaccgt gcctattcaa tggacagagt tcatttccga
      781 cacgaatcac actctggtct tgccggcaga cagccgggaa ggcgcattat tggggttgca
      841 gttctacatg gcggaaggag gagcgcttgt tgacagtaca ggccgccctc tgcttgagcc
      901 tgagcctctc gcccgggcgc tggccacaat aggtgtgcga aaggaaaatc tgctgcagag
      961 ccaccaactc aaaacgctcg atgaggcctg gcagtaccat caattaggat tgagcgattt
     1021 catgtggatg cgttccgagt ttttgttggg gcggcaggcg cttgatcctt cattactcaa
     1081 ttcacaaagt tacatggctg ttccaggact gaacggccac ctgttgccgt tagtgaccag
     1141 ttggtcgtgg gcgattacaa ctgaggaccc cgctcgacaa aaattggccg aggagttgat
     1201 cgtaaatctc acattgccgg aaaacctggc caattggggc cagaggagcc aggtattggc
     1261 tgcccagcga gcagcgatgg agatcatgag tgagcagaac ccgtacctgc aattcgcatc
     1321 gggccagttg gagttagcca gggcgatgcc ggttaatgaa acaagccggc tgatggatgt
     1381 gctgggcgac gcagtgttcc agctattgac gactgatact ccgccggaaa ttattgcgga
     1441 gcaggcggtt gctactttac gtcaatagca tgagacggca atttatacaa agggatagtc
     1501 ttccattgtc tggagcaaga tcccttccgg cttgggagag gcgggatcga gcagatggag
     1561 gtagacctcg acaatgtgcc gggcaataat cgaccagtcg aacttctccg cgctttttcg
     1621 tgccatacgc cccatttctg cgcggcagtc atcattgctc aagagttcgt agatcttcgc
     1681 agccaatgcc tcagggtcac gggtggggac gtgatagcca ttgaagccgt cgtgaatgag
     1741 aaatgccaga ccgcctactt ccgatgcaat gaccgggcgt cccatcgcca tcgcctcaag
     1801 ggcgaccatg ccgaagcttt cgtaatggga cggcatgacg accatctcgg ccgcggcata
     1861 atagttaggc agcacatcct ggtctttggc tcccaggaac atgatgatgt cgtggatgcc
     1921 taattcttgt ctcatggcct ggaggcgggt catctccgcg tccggattgt ctgcccaggg
     1981 atcaccgccg atgatggcga cgcacgtgtt cgcgacggag tcgggacagc gttgatggat
     2041 caacgccata gcctgaatga gcgtgtcgat gcccttcaat ggctcgattc gcccggcgaa
     2101 catgatggtt ttatccccgc aggggatgcc gacccgtttc tttgcctcgg ccttgtcgat
     2161 cggttgaaaa cgttccagat cgacgccggg cgggatgacg atgaccttct ccatatttgc
     2221 gccatagagc cagttgagtt gcgcttgttc ggcaggagtt gccgcgatga tctggttggc
     2281 gatagataat acatgacttt cgccgtcgat acgttcctgt ggcgcgcgtt cggcggcatc
     2341 cgtggcaata cggtttttca tatgaccgag cgtgtggaac atatggacaa tcggggtggt
     2401 gccccaataa tgttccagtt tttcggccac caagcccgac aaccagtaat gactatggat
     2461 gatgtcgtaa tgcaagttct cggtcgtcgc gaaattggcg ataccggctg cgaattcgtc
     2521 gatataattt cctacctcgc tgacgggtat gggagcttcg gggccggcgg ggatgtggat
     2581 cacgcgcgca ttgatcccaa gctcgtgatt gaccatcggc acgcaatcgt cttgtgaccg
     2641 cgtgaacaca tcgacgtgaa tccccatgct ccccagttct cggctcaagt cacggacgta
     2701 gacgttcatc ccgcctgttt tttttccgcc aagcatggcc aatggacatg tgtgcatgct
     2761 gatcatggcc acgcgaaggc cttcctgggg caggtgaaat ggattggttg attgacggta
     2821 ttcgttcatc atcatgcgat tcttcggatt ccagccttat ttttccatct ggatccggta
     2881 gatattggaa tcgaccgcgc caaagcgata tttatattct tcatnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnatatta taaagatcaa gcacgtgccg gcgatccggc
     3061 tgataatcct ggaggcagcg aatcgtcgct tgccatacct gctcaacatg gtcgtccctg
     3121 acgatcagat cgaggtaatc cgtctcctcc atgctgccgt taaaataaat cttgccgtca
     3181 tggatgtaga agcacccgat tcccagcaat tcacccttat cgcttcgcgt agcgacggta
     3241 cttaactcac tattgggtgg acagagatga gaccaccagg ccttctgata ggctaacctt
     3301 tgaaaaggtg tatttgtaat cccctgaccg gccaactggt cccattcgtg ggccaattcc
     3361 agaaagccgg gcaccccgct atatgaataa catttcatcg aatcgctcat atcaataatc
     3421 accaatattg ttcattatat atcactgtga tccgtgccaa cataggaaat acgacagagt
     3481 taagcaagag agatagcccg gcatgcattt aactcattcg ggactataat gatttgactc
     3541 cggctggaga tgtttttgag caacctgcgc tttagcaacg ccaaagcgta tagtggaaag
     3601 atcgccatga cagataaaac gcaggcgatg cgcgccctgg aagggaagcg tatcgcctat
     3661 gaagtgatga cctatcctga cgacatgcgc gatgcggaag agatcgcgct gtatttggat
     3721 atcgaacctg agacggtatt caagactttg gtcgtgatgc cccccgatcc gggtaaaaag
     3781 ccgttgttgg tcatggtgcc tgctgatcac cagttggatc taaagaaatt ggccgatgtg
     3841 gcaggggcga agaagctgaa gatggccacg catcgagagg cagagcaatt gaccggactt
     3901 caggtcgggg gcatttcggc cgtcgctctg ttggggaagc cgttcgtggt cttcatcgat
     3961 cgctccgcgg agcaacagag ccgcatcatc atcagcgccg gcaagcgggg cgtgcagctt
     4021 gtgctggctg tcaaggatct catcaagtta acgaatgccc gcgttgtgga tgtgagcgta
     4081 gtctgacgaa gaattggttt gtgcgggtat ggcgcatcaa aaaggccacc aaacggtggc
     4141 cttttgtgag aatctagaac aatccccaat cgcctccggc gccttcttcc atcatcgagg
     4201 ggtcccaaag ctcagtgcca atctcgactt taacgccgcc aggcataacc tgatttccga
     4261 cgattcgaac ctgctcgatt atttgcggtc cgtaagggca gaacggggtg gtgaggatca
     4321 tggttatgtt ggccgcatcg gtattgctat ctacttcaag atttcgcacc agtcccagct
     4381 cgacgatgtt taacccgatc tccgggtcga tcaccgatcg gagcgattcc atcaattgtt
     4441 cttctttact tgcgatcata ttggtcatac tctttcaatt tgcttctatt gccggaaccg
     4501 caggctccgt aacgtttgat tctattttaa tattgaattg gcaactgttg gccccatgaa
     4561 gcatacaggt ttcctggata acgtccagct gcagcacacc acgcatgagc tgtcgatcga
     4621 agttgcacac ctcgccgtga gcaccggtga ttgacaggta tgggcagcta tattccacga
     4681 gacgataccc atcagctgtg cgctcccagg cagacaggaa gccctcttcg gaaagcaggt
     4741 tcaccaaata atccagtcga tcttccaatg gcagatgttc aaattcgccg taatgttctt
     4801 ccaggacatt gtcgacgact cctttgaaaa tatcgtcaat gacggcagcc ggaagtctcc
     4861 ccttcatctc atcgagcaga cggctggtca ggcgcacata tcgcttgggg aatagctcct
     4921 gtcctttttc gctgagagaa tagacgtagt acggacggcc gacctttcgt cggacgctgg
     4981 atgcctcgat cgttccttca gccagcagtg cgttcaagtg atggcgcacg gtcaccggtg
     5041 atatatcggc cgcctcagcc aactcttcga cagtcgattg aggagactgt ttgatcgtat
     5101 ggagaatggt ttcacgagtt gttcgtttct tgcgattaac gaattcgtct gggttctgat
     5161 ccggcatatt catgggagga ctgctgctga cgctttgtga ggcgggttca ggaaagactg
     5221 aattgttccg ctaccaccgt ggatgcttga taacatcaac aatttgatta taacgaccgc
     5281 cttaagcaga gtcaatttcc tatattttta tcattaatat cataaatatt gacgggtgtc
     5341 aaccgcgatg ttataatctg ggtccagcta acattccatt accattcagc caatctatat
     5401 tcaaccgatc gaatcggaat agattgatat ttctaccgag gagcttactt gacgatgact
     5461 gctgcgttgg aaattaacga tctccatgtg aatattgggg aaaaccgtat cctgaaggga
     5521 atcaatttac aggttaagca gggcgagatt cacgccctga tggggccaaa cggctccggc
     5581 aaaagtacgc tggcctatac attagccggt cacccttcct acgagccgac ggccgggcag
     5641 gtgacttttg acggcatgga tcttctggag atggaagccg atgagcgttc ccgatccggg
     5701 attttcctcg ccttccagta tccggtggcc gtgcccggcg tgaccgtagc caaattcctg
     5761 cggcaggcga tcaattcacg ccgcaaggcc gagaatcctg aagataccgg catgcccatt
     5821 cctgagtttc gtcgcctgtt gcgcgcgaag atggatctcc tgggcattga tcagaaattc
     5881 gccgggcgct atctgaacga gggtttctcc ggtggcgaga agaagcgggc cgagattctg
     5941 cagatggcga tgctggagcc gaagattgcg atcatggacg agaccgactc aggtctcgat
     6001 atcgacgccc tgcgcattgt cgccgagggt gcgggccgcc tccacgagat taatgacatg
     6061 ggcgtcctcg tcatcaccca ttaccagaga atcctgaact acatccagcc taatcatgtc
     6121 catattatgc tcgacggccg catcgtcgag agcggcgggc cggagttggc tctccatctt
     6181 gaagagaacg gctatgactg gctgcgtgaa aagtatggta tggcggaggt tgcataatgg
     6241 ccgaccaggt ccttgaacgc gaactaactg aaaatgagat tgtcgcgggt gttaacgaag
     6301 attatgccgc caaatatggc tttgccgatg ttgaggatta tgttttcaag gccgagaaag
     6361 gcctgaacga gaatgttatt cgagccatgt cggccatgaa gcgcgagccg gaatggatgc
     6421 tcgaacttcg gctgaaagcg taccaatact tccttgagcg accgatgccc gaatggggtg
     6481 ccgatctttc cgacatcgat ttcgagaaca tctactatta catcaagccc acggagcgac
     6541 aggaagactc atgggaggat gttcccggct acatcaaaaa cacgttcgat aagctgggca
     6601 ttcccgccgc ggaacagaag ttcctttccg gcgtcgccgc gcaatatgag tccgaagtcg
     6661 tctatcacaa catcaagaag gaccttgagg cgaagggcgt aattttcctg ggtatggatg
     6721 atggtttgag acagtaccct gaccttgtta aggagtactt cagcaccatt attcccccga
     6781 acgacaacaa gtttgccgcc ctgaatacgg ccgtttggtc tggtggcagc ttcatctatg
     6841 tcccgcccgg cgttcatatc gacatgcctt tgcaggccta tttccgcatc aacgccaaga
     6901 attcggggca gtttgagcgc acgctcatca tcgtcgatga gggtgcctac gtccactacg
     6961 tagagggctg cactgcgccg atctattcga ctgattcgct gcactcggcc gtcgtggaga
     7021 tcgtcgtcaa gaagggtggt cgctgccgtt atacgaccat tcaaaactgg tccaataacg
     7081 tctacaacct cgtcaccaag cgggcaatgg cctatgagaa cgcgacgatg gaatgggtcg
     7141 acggcaatct cggctcgaag acgaccatga aatacccggc cgtctacctg atgggcgagg
     7201 gcgcccatgg cgagatcttg tcgatcgctt tcgcaggcac cggccagcat caggatgccg
     7261 gcggcaaggt cgtccacatg gctcctcgaa ccagcagccg catcatctcc aagtctatca
     7321 gcaaaggcgg cggccgcgca tcatatcgtg gacttctgaa ggtcgccgag ggcgcctatg
     7381 attgcaaatc caacgtggtt tgcgatgcgc tgttactcga cgagcatagt cgttccgaca
     7441 cataccccta tattgaaatc aacgagcagg acgtcaccat tggtcatgaa gcttcggtga
     7501 gcaaggtcgg cgaggaacag gttttctacc tgatgagccg cggcatatcc gaagacgagg
     7561 cgaacgccat gatcgtcaac gggttcattg aaccgttggt gaaagaactg cctatggagt
     7621 acgcgatcga aatgaatcgc ctgattcaat tacagatgga gggcagcatt ggctgagttg
     7681 cttcccttta accgcgatgc cgtcgttcaa ctgtcggcgt cgctgaatga acctgagtgg
     7741 atgctggatc tgcggttgaa ggcatgggag gtttttgctt ctctccccgt tccgacaacc
     7801 cgggaagagg cctggcggcg gacggatatt cgtcgcttca agctcgactc tttcggcata
     7861 tccctcaacg gcaacggcgc gaccggtgcc gatgtcccca gccacctggc cgcgcagctg
     7921 accgatgatg ccgccggcgg cgtgcttgtt gaagtgaatg gcctggtcaa ggagtactcc
     7981 ctcagtgacg agctccgcga gcaaggcgtt attttctgcg atatgcacac ggccgtacga
     8041 gagtatggcg atttggtgcg caaatacttt atgaccgaag ccgtccgtgc cgacgagggc
     8101 aagtttgccg cgttgcacgc tgctttctgg cgtggcggtg tgttcctcta cgttccaccc
     8161 aatgtctcgg ccgcggcgcc gcttcacagt gcgctgtggg ctacaagcgg ccgcacattc
     8221 acccatacgc tcgtcgtcct cgataccaac tccgaagcga ctttcatcga tgagtacgca
     8281 tcaccggcga agggcgaagg ccaggcgctg cataacggcg caattgagtt gatcgtccgc
     8341 gacggagcga gtttgcatta cgcttcgctc caggacttcg gcaccaacgt gtggcagttc
     8401 aatcacgagt gcggacgggt cggccgtgac ggcaaactgg actggataac cagcgtgatg
     8461 gggacgcgcc tgaccaaggc gttccagact tccgagctgg atgcccccgg cgcgtgggcc
     8521 cggatgtcgg gcattttctt cacgaatggc cgtcagcata tcgatctcga tacccagcag
     8581 aatcacaatg cggccgatac gaccagtgac ctgctctaca agggcgcgct caagggtgat
     8641 tcgcgctctg tctggcaagg gatgatcaag gctttgcccg gctcccagcg gattgatggc
     8701 ttccaggcta atcgcaatct gatgctcgac aggacggcgc gcgccgactc catccctggc
     8761 ctcgagattc aggccgatga tgtgcgatgt acccacgcct caacggtcgg caagctggac
     8821 caggaagaaa tcttctacct gatgagccgc ggcatccccc ggacggatgc gattcgcatg
     8881 gtcatccagg gctttttcga cccggtgatg cagcgaatcc cgttcgaggg cgtgcgcaca
     8941 caaatctttg gccgtattct tgagaagata ggctaattta gcctgctctc cgcaaacagc
     9001 tcgccgccgg ctattgatgc ttcgatgcgt attcgtgaag cgtcaatagt cggcgcttgt
     9061 tttagctctg ttcgcatata agtttggaga tgggaaatca tggatcaaaa cacttatgcc
     9121 cacgaggaag ttcttgtcag cacgcaatgg gttgcggata acttgcatcg caccgacaag
     9181 atcagattgg ttgagtcgaa tgaggacgtg ctgctctata gcaccggtca tattcccaat
     9241 gcggtccata tcgactgggt ggctgatctg aatgatgcgg tgcgccgtga ttatttgaac
     9301 gaggaacagt tcgccgcgtt gttgtcccgg aacgggattg acaacgatac gacggtcgtt
     9361 ttctatggcg acaagaataa ctggtgggcc acatatgcct tctgggtatt caagctcttc
     9421 ggccatgagg attgccgcat catgaacggc ggccgccaga agtggattgc cgaaggaagg
     9481 gagctgacca gagagaaacc gtcattcgcc ccgacagact acacgccggc tccccgcgtg
     9541 gattacaaga ttcgcgcttt tcgtgatcag gtcctggctc atgtgatggc cgaccaacct
     9601 ctcgtcgatg tgcgtagccc acaagagttc tccggagagc ttctccacat gcccggctcg
     9661 ccgcaggaag gcgcgctgcg cggcggccat atcaaaggtg ccgtgagcgt gccatggagt
     9721 caggcggtgg ccgaagacgg caccttcaag agcgctgagg cgttgaaatc gatctacgag
     9781 ggccgcgaag ggctttcgcc tgacgacaac gtgatcgcct actgccggat cggcgagcgc
     9841 agctcccaca cctggtttgt gctgacctac cttttgggct atgctaacgt tcgaaactat
     9901 gatggttcct ggactgaatg gggcaatctc gtgggtgtgc ccattgagaa cccttccagg
     9961 cagaaataag tgaattgtgt acgacgtaga tcgaattcgc gctgatttcc ctatactggg
    10021 cattgaagtt tatccaggcg ttcccctggt ttatctggac agcgccgcct catcccaaaa
    10081 accggcggcc gtcattgcgg cgatggatga atactatcgg caataccacg ccaatgtcca
    10141 tcgtggtgtg catcgattga gcgaactggc tacgaatgca tatgaatcgg cgcgggtccg
    10201 aatcggcacc ttcatcggcg cgccctctcc cgacgaaatc gtgttcgtcg gcaatacaac
    10261 cgaagctttc aacctcgtgg cctatagttg ggggcggcac aatatctccg caggggatga
    10321 gatcctcctg acggagatgg agcatcatgc caatatcgtc ccctggcaga tgcttgccca
    10381 ggggactggc gtgactattc gctatttgcc tgtcgacgag aacggccggc tggcgctgga
    10441 caagcttgac gactacctga ccgagcgcac acggctgttt tcattcacag gcgcctcgaa
    10501 cgtgcttggg accatcaacc ccgtgcgcga gttggttgaa gctgcccatg cggtcggcgc
    10561 tttggccatg gtggatgccg ctcaactcgt tccgcatatg ccgattgatg ttgaagctct
    10621 gggctgcgat tttctggcct tctcatccca caagatgtgc gggccgaccg ggatcggtat
    10681 cctctacggc aagcgcgagc ttctggaagc gatgcccccg ttcatgggtg gcggtgatat
    10741 gattcgccgc gtcacgttcg atggctttgt gcccaacgag attccgtgga aattcgaggc
    10801 cgggacgccc cggattgccg aggctgtggg gctgggcgcg gccgtcgatt acctcgattc
    10861 gctcggcatg gaagccgtac acgcccatga gttgtcgatc acgaattatg cccttgaatc
    10921 gttaagcgag atcccaggcc tcactgtgct cggcccaccg gcaacccaac ggggcgggct
    10981 ggtatcgttc accatgcagg gccttcaccc ccacgacata gcggaaatcc tcgacaaaga
    11041 cggcatcgcc atccgcgccg gtcatcactg cgccatgccc ctgcatcaga agctgggaat
    11101 cagtgcttcg gcgcgggcga gtttctatgt acatacgacg atggacgagg tcgataaact
    11161 gacgaccgct ctgcatcgcg ccagaagcgt gttcagattg gattgaatgg gatagagatt
    11221 tgtgcatata atcaagatgg ggatatgaca tggacgccaa tatctatcgc gagcaaatta
    11281 tcgatctcta tgaacaccct ctcaactatg gggagctacc gacgcccgat ttcacttatg
    11341 aggaagacaa cccgctgtgc ggagatgtca tccgcattga tgtcgttctt gatgagcaaa
    11401 agcgggtgaa ggaagttgcc tggcggggcg atgggtgtgc catcagtcag gcggccgctt
    11461 ccctcttgac agaagagatc aagggcaaga cgctcgatga agtaaaatca tttaccagcg
    11521 aggaactcct ggagcttgtc ggtgtgccgc taagcatggc gcgcgtgaag tgtgccttgc
    11581 tctcgcttaa agtcttgaag gccggagcct acggaatccc cgaccccgat accatacgga
    11641 aacatgagta ataagatacc gattgcacgc gaggatgcct tgcctcccgg ctcgcgattg
    11701 cactttgatc tggaggaaga atcagccatt ctcctgaata tcgacggaaa atattattgc
    11761 atcgccgacc tctgtacgca tgatggtggg ccgttggagg atggcgacgt gctggatttc
    11821 caaatagaat gtccacgcca cggcgcccga ttcgacattc gaaccggaaa agtcacgcgc
    11881 ctgccggcca ccgatcccat cccggtgtat cccgtatcaa ttgaagacgg gattgtctac
    11941 gtcgatgcgc ctga
//
LOCUS       DAOMQG010000069        11706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2364_length_11706_cov_11.093640, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000069 DAOMQG010000000
VERSION     DAOMQG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_11706_cov_11.093640"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLC06_05825"
     CDS             complement(<1..224)
                     /locus_tag="PLC06_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRO89999.1"
                     /translation="MSTLPIVAIPADILRAKTRPVTKFDAEIQRLIDNMIATMRQANG
                     VGLAAPQVGQPLKLAVIETLPEEDDEGNEI"
     gene            complement(227..1972)
                     /locus_tag="PLC06_05830"
     CDS             complement(227..1972)
                     /locus_tag="PLC06_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRO90000.1"
                     /translation="MTDKREFLAELPIFADLSTAALDAVARITREYAFKDNAIIAYQR
                     DLANRLYIVKEGRLFARAIDDNGIVRETYSFLPGQYFNENWLFIPGIHSATIKGKGRE
                     GGRLYIIEGPEFMQLLKHFPAIIDELAPREDDDVHYGLSDEAWGEAQKLKVQPRRMYS
                     SVAKAMLQNEHIELFTRRSWWFLLGQLIPPTLIMLFAAVLLVMIPADSPLERALKIGL
                     PVLTLLIGMIFFLLRLIDFRTDYFIITNKHVTHHEFELRHFRIHLVKVPIGQIQTVEI
                     MKPSFWATLFDIGTARVTTAAVAGVILFDNIDDPTAVKNTLERLSGQYKTVESAQTQA
                     VMRQSIENHFGLDPQLATTDAPVETPLRPVQRRGFFDNLGKQFGWRVVEGQTITYRKS
                     LFVLAKQVALPVLGFVILISLGMVGIYLKLPSWMLGAVIGIIGFFNLLWFAWQAENWR
                     NDIFQLTDRFVLDVDRKPFGFGESRKQAAISNIQNVDASRPGLLPTLFNYGFVSIDTA
                     GAKSDIVFDYVPNPELIQSDIFARIDETRRRDRVQEGSSRRKEYALLLDVYRQAMEQQ
                     RIPQRTPTDIPEDLE"
     gene            2245..3408
                     /locus_tag="PLC06_05835"
     CDS             2245..3408
                     /locus_tag="PLC06_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90001.1"
                     /translation="MAIQIARDWTVIRQEPLTREKQRLSEQAAFFLGVPPAVLVHESA
                     HALAIILFGGRVAEFGYRVFWGYVVPDGTFTAVENWIIAIAGTLGSLAFGGLLWLALR
                     HNKSRTVQYFGLRAFRFQIYFSLLYYPIFTLFLPIGDWKMIYDFSATPILSGVTLITH
                     AVLLLLFWRADRAGAFEMVAFDSAAAQMRHSAVQAAATTGNPEARLQMITELWSGGAH
                     QEARRELDALLAQYPTFAQGYLQRAVMVGGRQQLNKDAFEAAGKALELGLTNMSRRVL
                     AHQIRSLYQLQRGDGAAAQKELDAVLMPTAFNDPDEIIPIQRAELHQLRAQAFRRQGQ
                     YEAAYAEIEQALKLAREMGLEPAIQRYAEEKEIIEKHARRSFTDSPTIMDGKS"
     gene            3584..5047
                     /locus_tag="PLC06_05840"
     CDS             3584..5047
                     /locus_tag="PLC06_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO90002.1"
                     /translation="MQVELRDIRKYFGPVKANDGISLVLKPGKIYALVGENGAGKSTL
                     MKILSGYQPPTSGEILLDGQPVTFHSPADALAKGVGMLYQDPLDMPPFRVIDNYLLGR
                     DSRVRLNYKEARAELEGLAQRYGFHIDVNATIDTLSMGERQQLELVRLLAGGAQVLIL
                     DEPTTGISAEQKEILFDSMRKMTREEGKTVILVSHKLEEVQELCLYAYVLRRGKLAGE
                     QELPCLNEQLVEMMFGQVPQRSRRPAFDMGNPVLELQDVAIQTYQMNIPSINLSVRAG
                     EIFGFAGLEGSGQNLVMRACAGLLSPSRGRIRLKGQDVTGWNYHRRKKNGVAYVPAGR
                     LEEGLVAGLSLTEHLVLARPEKQFFVDWKGNQADMTERITTYQIVGRPETTADALSGG
                     NQQRLLFSLLDTPLNLLLLEQPTRGLDVRSSDYTWQLLYGRREHGTAILFISADLDEI
                     IAHSDRIAVFSGGVMSRVLDARDTSADELGHLIGGQV"
     gene            5044..6165
                     /locus_tag="PLC06_05845"
     CDS             5044..6165
                     /locus_tag="PLC06_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90003.1"
                     /translation="MIDTRRDGRAWLMRLWPLVPIVAALIFTSILLLLYNTSPAVAYR
                     AMWDGAFGNQDKFLSVMAFWIPLLLASAGLVVTFTAGLWNIGVEGQIILGAIGASWVA
                     LNIDGPAWMLIPLEIVAAMLCGALWAAIAAVLKTRGKVNEIFGGLALNNLAIILTNYL
                     VSGPWQPPEGGSFRGTVPFQEAALLPRLFESRFSPLSLILAAAAIAGVFVILRGTTWG
                     LKLKALGKNPYSAYLLGVSSERETVMAMMFCGALAGLGGAVRVLSWFDSLRQSISGGI
                     GFLALLIVMLAGYRILWVPLIAFFFSAVLNGSITLQLKTQLHSALGGILTGVMVLFVL
                     LFGSPRQLGFGRKQDMAGSPLETVPSAPENITTDLGGGD"
     gene            6165..7139
                     /locus_tag="PLC06_05850"
     CDS             6165..7139
                     /locus_tag="PLC06_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90004.1"
                     /translation="MDNAQLIAVLASIVLQASPLIIAVCGEILTERSGVVNLSLDGSM
                     LMSAMTGFLVGMKTDNVWLGFAAAAVVGMIFAAIVALGSIRLNQSQVAIGFVLTLLGD
                     DLSAFMGQNYTRMRGVIVPHYGIPLLKDIPVVGKIFFDQDIVVYFAMALIAATWWWLN
                     RTQPGLKLRGSGERPQAAFARGIDVNRQRYIYTLLGGALVGIAGAAYSLNVKIGWSDG
                     HIRGMGWIALAIVIFGGWSPIRGAFGAVLFGATKALATVLQRAFPEVSVVAFNSLPWI
                     LMIFVLLAVGSDYTERFINAMPQGWQRPLRRILRVSPPMALGTPFHEG"
     gene            7163..8383
                     /locus_tag="PLC06_05855"
     CDS             7163..8383
                     /locus_tag="PLC06_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90005.1"
                     /translation="MVDVFIKYPRLGRLFDLALPLLATLAALGVGAIMLQLLGANPIK
                     AYGALLNGAFGSTNAVADTIVRATPLLLVALGICIAFRAGVINIGGEGQLVAGAILAT
                     LIGLKMPDAPGIVIIPLALIAGFVGGAIWGGIPGVLKAYYNVNEILSTIMLNIIAVQG
                     MNYLLRGPMIDPAQMEVSSRIPQTARLAQAFDLPRWVPTRLHLGALIAVILAFLVYIL
                     LWRTTVGYRIRAVGLNPEASRYAGIRVRRNIVLALLLSGAFAGLAGAVQVYGVHHRMF
                     TDGSLAGFTGSAGFNGIVAALFGKLNPIGAIPASFIFGALIVGANSLQRAMQVPAALI
                     TALNGLVVVFVVSSDIFSRRLARSRQIGAEASAAETGTNIKPDIGAGSKPAPTGNVPA
                     EAPPALNAEGEASK"
     gene            8380..9360
                     /locus_tag="PLC06_05860"
     CDS             8380..9360
                     /locus_tag="PLC06_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90006.1"
                     /translation="MISDLFTTTVLIGVLTSGIRLATPYLFASIGEMFGQRSGIYNLG
                     VEGIMLMGAFAAFYVTLTNGNPWLGLLAAIIVGGVMGLAMAFISVTLQAEQGISGIGV
                     YLFGLGMSELLFQKMLGTVETVSGFRPVSDFLTRHFPDLMSTLLGIPVLGAIVSILAQ
                     QSIIVYFAYALVPVAWFVLYRTTLGLKIRAAGENPAAADALGVSIPRTRYATVTFGGV
                     MAGIAGASLSIALLNVFQQNLTSGLGFIAVALVYFGGWKPYGVLFGALLFSMVNALQL
                     WIQALNIPIPGNASELLIMMPYVLTIVVLTFSAGRMRKPAALGVPYERGS"
     gene            9587..>11706
                     /locus_tag="PLC06_05865"
     CDS             9587..>11706
                     /locus_tag="PLC06_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRO90007.1"
                     /translation="MKRYAWLALLLIFAMAIVACGGTRPATEAPTTGGEEAAPTEAAA
                     AEAPAEEASGPFRVAVVMPSAINDLAFSQSMYDTLVAIQNEMGAENFEFAYSDNMFVV
                     DDAATALRDYASQGYNLVLAHGSQYGSSLQEIAPDFPETSFAWGTTVDTFGIPNIYAY
                     EARSEEGGFVNGVIAATLSKSNMLGVVGPIETGDAKLYVDGFAAGAKSVKPDITVNVN
                     YIGSFSDVALAAEAANTQVGAGADALTGTAQMVVGAIGVAEENGIPWFGTQSSQTSLA
                     PDIVVASQVYNWKGVINTIIENVKKGELGGTSFALTLGNGGLVMDYNPAYTLPAEAKA
                     AADAAVAGISAGTLSVFGGETAAPAEEPAAEAGPIKFGMILVGPKNDHGWSQANADGG
                     QFVLDNLPGSEMILFESLNPADKPEATVEGVVDDMVAAGATLIFATSDEFEEDTLSAA
                     LKYPDVTFIAVTGDDVKTGEAPPNLGNTMSRMYLMKAVAGCAAALGTETGNIGYLGPL
                     INFETRRLAASAYLGAKYCYENYRGEDPANMGFAVNWIGFWFNIPGVTLDPTEVVTNF
                     IDTGTDVILSGIDTTEAIDISGQRAAQGDAVWAIPYDYLAACDNAPDICLGVPFFSWG
                     PSYLNIAKAVSNGTWVQAWDWFPPTWDSLADPAITDVGWINGPALTDEMKAQLDEFIA
                     GMASGEINVWTGPINLQDGTSYIA"
BASE COUNT         2269 a         3452 c         3507 g         2478 t
ORIGIN      
        1 tcgatctcgt tgccctcatc gtcttcttcg ggcagcgtct cgatgacggc cagcttgagc
       61 ggttggccga cctgtggggc agccaggccc acaccattgg cctggcgcat tgtcgcaatc
      121 atgttatcga taagtcgttg tatttcggcg tcgaatttgg tgaccggcct tgttttggcc
      181 cgcagtatat cggcggggat ggccacgatt ggcaaagtgc tcatagttac tcaagatcct
      241 cagggatatc ggtgggagtg cgctgcggga tgcgctgctg ttccatggcc tggcggtata
      301 catcgagcag cagggcatac tccttccgtc gggagctgcc ttcctgaacg cgatcccgcc
      361 ggcgggtctc gtcgatacgg gcgaagatgt cgctctggat cagctccggg ttaggtacat
      421 agtcgaacac aatatcggac ttggcgccgg ctgtgtcgat gctgacgaag ccatagttaa
      481 acagcgtggg caaaagcccc ggccgcgatg cgtcgacatt ttggatattg gagatggccg
      541 cctgcttgcg actctcccca aagccgaacg gcttgcggtc aacatcaagg acgaaccggt
      601 cggtcaactg gaagatatcg ttacgccagt tctccgcttg ccaggcgaac cacaaaagat
      661 tgaagaaccc gatgattccg atgacggctc ccagcatcca cgacggcagc ttcagataga
      721 ttcccaccat gcccagcgag atcaggataa caaagccaag cacgggcaag gcaacctgtt
      781 tggccaacac aaaaaggctc ttgcgatagg tgatcgtctg cccctcgacc acgcgccagc
      841 cgaactgttt gcccaaatta tcgaagaaac cccggcgctg cacgggacgg agcggagttt
      901 caaccggcgc atcagtcgta gccaattgtg gatcgaggcc aaagtgattc tcgatcgatt
      961 gacgcataac cgcctgggtc tgggccgatt cgacagtttt atattgcccg ctcaggcgct
     1021 cgagcgtatt ttttacggcc gttggatcgt cgatgttgtc gaacaggatc acccccgcca
     1081 cggcagccgt cgtgacccgt gccgtgccga tgtcgaacag cgtggcccaa aacgaaggct
     1141 tcatgatctc aaccgtctgt atctggccga tgggcacctt caccaaatgg atgcggaaat
     1201 ggcgcagctc gaattcatga tgggttacat gtttgttggt gatgatgaag tagtcagtgc
     1261 gaaagtcgat gagtcgcagc aagaaaaaaa tcatgccgat gagcaaggtg aggaccggca
     1321 gaccgatttt gagcgctcgt tccaacggtg aatcggccgg gatcatcacc agcaaaacgg
     1381 ccgcgaacaa catgatcaac gtcggcggta tgagctggcc cagcagaaac caccaactgc
     1441 ggcgggtgaa cagctcaata tgctcgttct gcagcatggc cttggccaca gacgagtaca
     1501 ttcgccgcgg ctggaccttc aacttctggg cctcgcccca cgcctcatcg ctcaggccgt
     1561 aatgaacgtc gtcatcctcg cggggagcca gctcgtcgat gatcgccggg aaatgcttca
     1621 gcagctgcat gaactccggc ccctcgatga tgtagaggcg gccgccctct cgccctttgc
     1681 ctttgatggt cgccgagtgg atgccgggga tgaagagcca gttctcattg aaatattgcc
     1741 ccggcaggaa ggaataagtc tcacggacga taccgttgtc gtcgatggcg cgagcaaaga
     1801 ggcgaccttc cttgacgata tagaggcgat tggcgaggtc gcgctggtag gcgatgatgg
     1861 cgttgtcctt gaaggcgtat tcccgcgtga tccgcgccac agcatccagc gcagccgtgc
     1921 tcaggtcggc gaaaatcggc aattcggcta gaaactcgcg cttgtcggtc acgcctccat
     1981 tttaacatgg cgaccaaggg atgactaatc gccattattg cctgacggct acggtcaaaa
     2041 ctcccctgcg gtaacaggat cccgcaccac acaccagcca caccttcaat ttgatcactg
     2101 attcacaaat tggtatgaca atcacaccat ttattattgc cgacagaaat aatgtccgtt
     2161 attatcagca aaggtctgcc aatgtccttg ataacgggtc tattcaacgt tctcgccctg
     2221 ttatacattg gccgagccgt tctattggcg attcaaatcg ctcgcgattg gaccgtcatc
     2281 cggcaggagc cacttaccag ggaaaagcag cgcctgagcg aacaggcggc atttttcctt
     2341 ggcgtccccc cggctgtgct tgtgcatgaa tcggcccacg ctctggctat catcctgttc
     2401 ggcggccgtg tggcggaatt cggctatcgc gtgttctggg gctatgtcgt gcccgatggg
     2461 acatttacgg ccgtcgagaa ctggatcatc gccatcgccg ggacattagg atcgctggct
     2521 ttcggcggcc tgttgtggct ggccctgcgc cataacaaga gccggacggt acaatacttc
     2581 ggcctgcgcg cttttcgctt ccagatctat ttctcgctgc tctattaccc catcttcacc
     2641 ctgtttttgc ccatcggcga ttggaagatg atctacgatt tttcagcaac gccaatattg
     2701 agcggcgtga cgctgatcac ccacgccgtc ttgttgctcc tgttctggcg cgccgaccgc
     2761 gctggagcat ttgagatggt cgccttcgat tcggccgccg cccagatgcg ccattccgcg
     2821 gttcaggcag ccgccacgac aggcaatccc gaagcacgat tgcagatgat cacggaactg
     2881 tggagtggcg gcgcccacca ggaagcccgt cgcgagctgg acgccttgct ggcccagtac
     2941 cccacattcg cccaaggtta cctgcagcgg gcggtcatgg tcggcggaag gcaacaatta
     3001 aacaaggacg cttttgaggc cgccggtaaa gcgctggagt tgggcctcac caatatgagc
     3061 cggcgggtac tggctcatca aatcaggagc ctctaccaac tgcagcgcgg cgacggcgcg
     3121 gccgcccaaa aagagctgga cgcggtgctc atgccaaccg ccttcaacga cccggatgag
     3181 atcatcccga ttcagcgggc cgaactgcat cagttacgcg cccaggcatt tcggcgacag
     3241 ggacaatacg aagcggctta tgccgagatt gaacaggccc tcaagttggc ccgcgaaatg
     3301 gggctggaac ccgcgatcca gcgttacgcg gaggaaaaag agatcatcga aaaacatgcc
     3361 cggcgcagtt tcacggactc gccgaccata atggatggga agtcatgagc agacacaagg
     3421 attccgacgg cctgaagtct gcatcattac gatgaatgtc atatcatact caggatcaat
     3481 gacagtgtac ctggttgaac gtaaccggta tccttagggt acgcattggc cttgcgccga
     3541 cctgcccttg aatttcgccg gacattttcc attgagaggt cctatgcaag ttgagcttcg
     3601 ggacattcgc aaatattttg gcccagtgaa ggccaacgac ggtatcagcc tggtgttgaa
     3661 gccgggcaag atttatgcgc tcgtggggga gaacggggca ggcaagagca ccctgatgaa
     3721 aatcctctcg ggctaccagc cgcccaccag cggcgagatc ttgctcgacg gccaaccggt
     3781 gacttttcac tccccggccg atgctctggc caagggggtc ggtatgctgt atcaggaccc
     3841 cctcgacatg ccgccgttcc gcgtgatcga taactacctg ctcggccgcg acagccgtgt
     3901 gcgcctcaac tataaagaag cgcgtgccga actggaaggg ctggcccagc gctatgggtt
     3961 ccatattgac gtcaacgcga ccatcgatac attgtcgatg ggcgagcgac agcaactgga
     4021 gttggtgcga ttgctggccg gtggagccca ggtgctcatc ctcgacgaac caaccacggg
     4081 catcagcgcc gagcaaaaag agatcctctt tgactccatg cgcaaaatga cccgcgaaga
     4141 aggcaaaacg gtcatcctcg tttcccacaa gctggaagag gtgcaggaac tatgcctcta
     4201 tgcctatgtc ctgcgacgcg gcaaactggc gggggagcag gaattgccgt gcctgaatga
     4261 gcaactggtc gagatgatgt tcggtcaggt accgcagcgg agccggcgac ccgcgttcga
     4321 catgggcaat cccgttctgg agctacagga tgttgccatc cagacctatc aaatgaatat
     4381 cccctcgatc aatctctccg tgcgtgccgg ggagattttt ggttttgcgg ggctggaggg
     4441 cagcggtcaa aacctcgtca tgcgagcatg tgccggattg ttatccccat cccgggggcg
     4501 catacggctg aagggtcagg acgtgaccgg ctggaattac catcgccgga agaaaaacgg
     4561 ggtggcctat gtccccgccg ggcggctgga agaagggctg gtcgcagggc tatcgctgac
     4621 cgaacatctg gtcctggcgc gccccgaaaa gcagttcttc gtggactgga agggaaatca
     4681 ggccgacatg accgagcgca tcaccactta tcagatcgtc ggccgccccg aaacgacggc
     4741 cgacgcgctg tcgggtggca atcagcaacg actcctgttc tccctgctgg atacgccttt
     4801 gaacctgctg ctgctggaac aacctacacg cggcctcgac gtgcgttcgt cggattacac
     4861 gtggcagttg ctctacggac ggcgcgaaca tggaaccgcc atcctgttca tttcggccga
     4921 cctggatgag atcatcgccc acagcgaccg aattgccgtg ttctccggcg gcgtgatgtc
     4981 tcgcgtcctt gacgcccgcg acacctcggc cgatgaactg ggccatctca taggaggaca
     5041 agtatgatcg atacccggcg agacggccgc gcctggctca tgcgcctttg gcctctggtg
     5101 ccaatcgtgg ctgcgttgat cttcaccagc attttacttc tcctgtacaa caccagcccg
     5161 gcagtcgcct accgggccat gtgggacgga gcgtttggca accaggacaa gttcctgagc
     5221 gtcatggcct tttggatccc gctgctgctc gcatcggcgg ggcttgtcgt caccttcacc
     5281 gccggcctgt ggaacatcgg tgtggaaggt cagatcatcc tgggagccat tggcgccagt
     5341 tgggtcgccc tgaatataga cgggccggcc tggatgctca tcccgctgga gatcgtcgca
     5401 gcgatgctct gcggagcgct ctgggcagcg atcgcggccg tcctgaagac acgtggtaaa
     5461 gtgaacgaga tttttggtgg tttggcgctc aataatctgg ccattatcct gacgaactat
     5521 ctggtctccg gcccatggca gccacctgag ggggggtcgt tccggggaac cgtgcccttc
     5581 caggaagcgg ccctgctgcc ccgacttttt gagtcacggt tcagcccgct ctcgctcatt
     5641 ctggccgcgg cggcgatcgc cggggtgttc gtcatcctgc gcggcacgac ctggggattg
     5701 aagcttaaag cgctgggcaa gaacccgtat tcagcttacc tgttgggtgt gtccagcgag
     5761 cgcgagacgg tgatggccat gatgttctgc ggtgctctgg ccggtctggg tggcgcggtg
     5821 cgcgtgctca gctggttcga cagcctgcga caaagcatct ccggcggcat cggctttctg
     5881 gccctgctca tcgtcatgct cgccggctac cgcatcttgt gggtgccgct gatcgccttc
     5941 ttcttttcgg cggtgctcaa cggtagcatc acgctccagc tgaagaccca actccactcg
     6001 gcgctgggcg gcatcctgac cggagtgatg gtcttgtttg tgctgctatt cggttcgccg
     6061 cgccaactgg ggttcggccg gaagcaggac atggcgggta gccccttaga gactgtcccg
     6121 tcggccccgg aaaatatcac aaccgatctg ggaggaggcg attgatggat aatgcccaac
     6181 tgatcgctgt actcgcatct atcgtgctgc aggcctcgcc gctgatcatc gccgtttgcg
     6241 gcgaaatcct gaccgaacgc tccggcgtcg tcaacctgtc gctcgatggt tccatgttga
     6301 tgtcggccat gaccggcttt ctggtcggca tgaagaccga taacgtttgg ctgggctttg
     6361 cggcagcggc cgttgtcggc atgatcttcg ccgcgattgt tgccctcggc agcatccgcc
     6421 tgaatcaaag ccaggtcgcc atcggcttcg tgctcacgct gctgggcgac gatctgagcg
     6481 cctttatggg tcagaattac acgcgcatgc gcggcgtgat tgtgccccac tacggcatcc
     6541 ccctgctgaa agatattccc gtcgtcggca agatattctt cgatcaggat atcgtcgtct
     6601 acttcgccat ggcgctcatc gccgccacct ggtggtggct caaccggacg cagccgggtc
     6661 tgaagctgcg cggctcgggc gaacgccccc aggcagcttt tgcgcgcggc atcgatgtga
     6721 atcgtcaacg ctacatctac accctgttgg gcggcgcgct ggtcggcatc gccggagcag
     6781 cctactcgct taacgtcaaa atcggctggt ccgacggcca tatccgcggc atgggctgga
     6841 tcgctctggc catcgtcatc ttcggtggct ggtcgccgat tcgcggcgcg tttggcgcgg
     6901 tgcttttcgg cgcaacaaaa gcgttggcga ccgtgctgca gcgcgcattc cccgaagtat
     6961 cggtggtggc cttcaatagc ctgccctgga tcctcatgat ctttgtattg ctggccgtcg
     7021 gcagcgacta cacggaacga tttatcaacg ccatgcccca gggctggcaa cggccgctac
     7081 gccggatatt gcgcgtctcg ccaccgatgg cgctcgggac gcccttccat gaagggtagc
     7141 atcacgcaac ttaaggtgac ttatggttga tgtcttcatc aaatatccac gcctcggacg
     7201 attattcgac ttggccttgc ccttgctggc cacgctggcc gcgctcggcg tgggggccat
     7261 catgctccaa ttgctgggcg ccaacccgat taaagcctat ggggccttgc tgaacggcgc
     7321 atttggcagc accaacgccg tggccgacac catcgtgcgg gccacgccgc tgctgctggt
     7381 cgccctgggc atctgcatcg cctttcgcgc gggtgtcatc aacatcggcg gggaagggca
     7441 gttggtggcc ggggccatac tggcgacgct catcggcctg aagatgccgg atgcgccggg
     7501 catcgtcatc atccccctgg cgctcatcgc cggcttcgtg ggtggggcca tttggggcgg
     7561 catccccggc gtgctgaagg cgtattacaa cgtcaacgaa attctgagca ccatcatgct
     7621 caacatcatc gccgtgcagg ggatgaacta cctgctgcgg gggccgatga tcgacccggc
     7681 ccagatggaa gtgtcgtcgc gcatccccca gacggcacga ttggcccagg cgttcgatct
     7741 gccgcgctgg gtaccgaccc gacttcacct cggcgcgttg atcgctgtga tcctggcctt
     7801 cctggtctac atcttgttat ggcgcacaac cgttggctat cgcattcggg ccgtgggtct
     7861 gaaccctgag gcctcgcgct acgcgggcat ccgggtgcgg cgcaacattg tcctggcttt
     7921 gctgctcagc ggcgcgtttg ccgggttggc gggagcggtg caggtctatg gcgtgcatca
     7981 tcgtatgttt accgacggct cactggccgg gttcaccggc tcggccggat tcaacggcat
     8041 tgtggcggcc ttgttcggca agctaaaccc gattggcgcg ataccggcgt cattcatatt
     8101 tggcgcactc atcgtcggcg cgaacagtct acaacgagcc atgcaagtgc ctgcggccct
     8161 gattacagcg ttgaacggac tcgtagtcgt cttcgtcgtc agcagcgata tatttagtcg
     8221 tcgattggcg cgcagccggc agatcggcgc ggaggcgagt gcggccgaga cagggacgaa
     8281 tattaaacct gacatcgggg cgggttcgaa accagcccct acagggaatg tgccggcgga
     8341 ggcgccacct gcactgaatg cagagggaga ggcatccaag tgattagcga tctgtttacc
     8401 acaaccgtac tcattggggt gttgacctcc ggcatccgac tggcgacccc atacctgttc
     8461 gcctctattg gtgaaatgtt cggccagcgc agcggcatct ataatctggg tgtcgagggc
     8521 atcatgctca tgggcgcctt cgccgctttc tatgtgactt taaccaacgg taatccgtgg
     8581 cttggcttgc tggccgcaat tatcgtcggc ggcgtgatgg gcctggcgat ggccttcatc
     8641 agcgtcacgc tgcaagccga gcagggcatc agcggcattg gcgtctacct gtttggcctg
     8701 ggtatgagcg agttattgtt tcagaagatg ctcggcacag ttgaaacggt gagcggtttc
     8761 cgcccggtca gcgacttctt gacccggcat ttccctgacc tgatgtccac gcttctgggg
     8821 ataccggtgc tgggtgccat cgtctcgatc ctggctcagc aaagtatcat cgtctacttc
     8881 gcctatgcgc tggtgccggt tgcctggttt gtgctctatc gtacaacgct ggggctgaag
     8941 attcgcgccg cgggcgagaa cccggccgcc gccgatgccc tgggtgtcag tatcccgcgc
     9001 acccgttatg ccacggtcac gtttggcgga gtgatggccg ggatagccgg tgcttcgctt
     9061 tccattgcgt tactcaacgt gttccagcaa aatctgacca gcgggctggg cttcatcgcc
     9121 gtggccctgg tctattttgg cggctggaag ccgtatggcg tcttgtttgg cgcgttgctg
     9181 ttcagcatgg tgaatgccct ccagttgtgg atccaggcgc tcaacatccc gattcccggc
     9241 aacgcgtcgg agttgctcat catgatgccc tatgtgttga ccatcgttgt ccttaccttc
     9301 tcggccggcc gaatgcgtaa acccgcggct cttggcgtac cttacgagcg aggcagttaa
     9361 ccggacgcgc cgaaacacac ataaaggagg tgatgtcgtc ccaaattaca ccatgagtct
     9421 tgtttacaat cgaccaatcg gcgttcgatg ggtcaccctt gcaggccgct gtcgatccac
     9481 ccacaccttg tgaatgaccc tattgtgtgg ccacattgtg tggttgaaga acggtggaga
     9541 atagattctc atgtggaaac caattgtttc aatggagaag aagaaaatga aacgttatgc
     9601 atggctcgct ttattgttga ttttcgcgat ggctatcgtg gcctgcggag gtacgcggcc
     9661 ggcgaccgaa gcgccgacta cgggcgggga agaagccgcg cccaccgagg cggcggctgc
     9721 agaggccccc gccgaagagg ccagcggccc gttccgtgtg gccgtggtca tgcccagcgc
     9781 catcaatgac ctggccttca gccagagcat gtatgatacg ctggttgcca tccagaatga
     9841 gatgggggcc gagaacttcg agttcgccta ttccgacaac atgttcgtcg tggacgatgc
     9901 cgcgacggca ctgcgcgact acgccagcca agggtacaac ctggttctgg cccatggctc
     9961 gcagtacggc agctccctgc aggagatcgc tcccgatttc cccgaaacga gctttgcctg
    10021 gggcacgacg gtcgacacct ttggtatccc caatatctac gcctatgagg cgcgttcgga
    10081 agaaggtgga ttcgtcaacg gcgttatcgc cgctaccctg tccaagagca atatgctggg
    10141 cgtggtcggc ccgatcgaga cgggtgatgc caagctgtat gtcgacggct ttgccgccgg
    10201 cgccaagtcg gtcaagcctg acatcaccgt caatgtcaac tacatcggtt ccttcagcga
    10261 cgtcgctctg gcggccgagg cggccaatac ccaggttggc gccggcgcgg atgccctgac
    10321 cggcacggcc cagatggttg tcggcgcgat cggcgtggcc gaggagaacg gcatcccgtg
    10381 gttcggcacg caatcgagcc aaacctccct ggctcccgat atcgtcgtcg ccagccaggt
    10441 ttataactgg aagggcgtca tcaacacgat catcgagaac gtgaagaaag gcgagttggg
    10501 tggcacatcc ttcgctctga cgctggggaa cggcggacta gtgatggatt acaaccccgc
    10561 ctacacattg ccggcagaag ccaaggccgc ggccgacgcg gcagtcgcgg gcatctccgc
    10621 gggcacgttg tcggtattcg gcggcgagac ggccgctccg gccgaggagc cggcggctga
    10681 ggcaggccct atcaagtttg gcatgatcct ggtcggcccg aagaatgacc atggctggag
    10741 ccaggccaac gccgatggtg gccagttcgt ccttgataac ctgcccggtt cggagatgat
    10801 cctcttcgag tctctgaacc cggccgacaa gccggaagcg actgtggaag gagtcgtcga
    10861 tgacatggtg gccgccggcg caacgcttat cttcgcgacc tctgacgagt tcgaggaaga
    10921 tacgctcagc gcggccctga aatatccgga cgttactttc atcgccgtca ccggcgatga
    10981 cgtcaagacc ggcgaagcgc cgcctaacct gggcaacaca atgagtcgta tgtatcttat
    11041 gaaggctgtt gccggttgtg cggccgcgtt gggcaccgaa acgggtaaca tcggctacct
    11101 tggcccgctg atcaattttg agacacgtcg tctggcagcc tcggcctatc tgggcgcgaa
    11161 atattgctat gagaactatc gtggcgaaga tcccgccaac atgggcttcg ccgtcaactg
    11221 gatcggcttc tggttcaata tccccggcgt cacgctggac ccaaccgaag tggtgaccaa
    11281 cttcatcgac acgggcacgg atgtgatcct cagcggcatt gacaccacag aagcgattga
    11341 catctccggc cagcgcgcgg cgcagggcga tgccgtttgg gccatcccct atgactatct
    11401 ggccgcatgc gacaacgcgc cggatatctg tctgggcgtc ccgttcttca gttgggggcc
    11461 atcctatctc aatatcgcga aagccgtttc caacggtacc tgggtgcaag cctgggactg
    11521 gttcccgccg acctgggata gcctggccga tccggcgatt acggatgtcg gctggatcaa
    11581 tggcccggct ctgaccgatg agatgaaggc acagctcgat gagttcatcg ccggcatggc
    11641 cagtggtgag atcaacgtct ggaccggccc gatcaacctg caggacggca cttcctatat
    11701 cgccga
//
LOCUS       DAOMQG010000070        11616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2390_length_11616_cov_10.118848, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000070 DAOMQG010000000
VERSION     DAOMQG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_11616_cov_10.118848"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1381
                     /locus_tag="PLC06_05870"
     CDS             263..1381
                     /locus_tag="PLC06_05870"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90008.1"
                     /translation="MFSHEHMQELVTYAGNGRGVVSVYLDTDNTRQSMEAIKLTARGL
                     LKEVQPQYDREAQAIERYLDLSYDWSKPGLALFCGKGGEFFRAYDSPVAFRNRVRLST
                     RPYVKPLTHLLDHYAHFGVIVVNQVGGEFYAYHLGEIQAGDIYTGEDVRRIKDGGGSS
                     AVGRRGGSGSGGPGGSKHEDEVAARNMRECAAAANQFFSEYPIRRLFLAGTNENVAQF
                     RELLPKQLQSCLVGSFPVGNDAGEPEVRQRALNLLREVNAEREAKLVDRLITLNAQGG
                     NAVIGLDAALQAVSDKRVDTLIISDGYRAPGYFHEPSGFVVANLTRSPLTEADLTEAA
                     DVIETAVAQTVAQGGHVEVIAENPYLEDAGRIGAILRY"
     gene            complement(1529..3115)
                     /locus_tag="PLC06_05875"
     CDS             complement(1529..3115)
                     /locus_tag="PLC06_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90009.1"
                     /translation="MTTGEYDRLSGILREWDGRRRLQELLQYLPLGLAVGLFLGLVAA
                     LLSRARPLLLRGELALLTLAAALIAISVTGLIVMARRRSPVDQARFADRRFNLRERMT
                     AAIEIQSGQLAVDDAMATRQLDDALRAASAVDANRELPLRSRLSDWLPAFAGVALLAL
                     ALWLPNPQEAVVLEQRAVTAAVVEQSQALAELIDEIKAADTLTAEQQEALTQPLEEAL
                     TALSRLNLSREEAVAALSAAETEMRALSQEMDPASLREALAAAAMDQPGELAEALRAG
                     QLDQAAAATSALAASLGDLDAGAQAALAEQLAATANALESADAELAETLDRAAEALAA
                     GDTGTAREALDEAAGQLANRAQTAGAAAQASATADRLGQARGEVAQSGAGGQPGGTGT
                     GTGESSTSGDAGEGGTGQGGTSESSGSSTGTQTGGTGGPSPGGGHVESVFVPQRPDLE
                     GEGEGVELDVQCLTDPETCGPLAGQSPSSLEGRPGGSLVPYDQVFGNYRDAAFEALSQ
                     GNIPIGLQNLVRDYFSALEP"
     gene            complement(3112..4047)
                     /locus_tag="PLC06_05880"
     CDS             complement(3112..4047)
                     /locus_tag="PLC06_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90010.1"
                     /translation="MTAETTTPSPTSRRPWTALLNNPVMTKELRSRMRGHRAFAVLTL
                     YLIVLSAAILFVYLIYLSSAAVPSNTMARTTGKGLFAAVLGVQIILVTFIGPAFTAGS
                     ISGERERQTFDLLRTTLLPANAFVLGKLVSALSYIFLLVIVSIPLQSLAFLLGGLSPV
                     ELVLSQVLVLITAAPYALYGLWCSAAMRSTLTATVATFAGTLFLTFGIPMLAFVAIMM
                     LGFTGFSIGTPGPTLEMILIYVGLVLAATNLPATLVISEAFLLSQDTLLGYTYHGTQL
                     MWIFSPWWLFIIFHVLAAVILYLATVRRVRRISDV"
     gene            complement(4090..5049)
                     /locus_tag="PLC06_05885"
     CDS             complement(4090..5049)
                     /locus_tag="PLC06_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90011.1"
                     /translation="MTAIIELRDLTKRYGDLKAVDSLDLAIDQGAVFGFIGPNGAGKT
                     TTMRILTTLLRPTSGEARIAGYSVADDPRDVRRVIGYMPDYFGVYEDMKVWEYLDFFA
                     ACYDVPAPTRPGMIDDLLALVDLGHKRDEYVESLSRGMKQRLCLARTLTHDPQVLILD
                     EPASGLDPRARIEMRELLRELQKMGKTIFFSSHILAEVADICTSIGIIENGRLVATGN
                     VDEMMARLRGHRLIAMRFVSGAAVDANEAANWLRGRPGVLSATVESGNPSSGMRLDFN
                     GDDQALSDLLAGAIAHGLPVIGFNEERGDLEDVFLQVTQAAAQ"
     gene            complement(5056..7029)
                     /locus_tag="PLC06_05890"
     CDS             complement(5056..7029)
                     /locus_tag="PLC06_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90012.1"
                     /translation="MFLSFLLSRLFVILLLPFAQDGGLTLEAQAGFDGLYETSLTVPV
                     VVTARNGGEPFDGEIRIISPGASGANALVYSAPLTLPTGADKRVPLVVYTESFGNRLT
                     AQLVAGGRVVAEVETNRLTGLGRNDLLYGVVSPEPGGLAFLETVQGSRSDAAVAFLTL
                     DDLPEVSSAWDALDILVLDDIDTSRLTSGQLAALRAWIESGGQLVVTGGPGGPKTAAG
                     VAELLPVAVTGVESLADLAALGDFAGAPLSGDGPFVATTGNLIAGEVLIEQNGLPILA
                     RRDIGRGSVFFLALDPKIAPLSSRPGNEALWTAIIANAPKLPPWGWGVQDTYAASQAI
                     SYIPGLSLPSAWQLFFFLLVYVLVIGPINYIILRRARRRELAWVTIPALVLLFSAITF
                     ATGFRARGNDATLNVMSVAFGSADADRLQTQSLIGLFSPRRTTYDLTLPYDSSTFPFQ
                     PGFGTLVSGGNLAAIERSGQVLLRDVRTDTSQIATFVVNAHLPRPAISAGATRSADGS
                     GLVVTVRNGTADTLENAVILHGQEQITLGNLAPGEEKSLSVPLGESAAASPTPDPMFS
                     AGFINPNPLITDPSLILGTYDYFSDPVAYPRWQLIQAHYMGEDYDPTTLPDPTKVVTL
                     GGWLAGSAQEVTSSAAGTKRLATTLLLLEIPIR"
     gene            complement(7033..7317)
                     /locus_tag="PLC06_05895"
     CDS             complement(7033..7317)
                     /locus_tag="PLC06_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HRO90013.1"
                     /translation="MFVGTCIITLELDGVRSLKEKRSIVKSIIARLQREFNLSVAEVD
                     GQDAWGTAVIGLAVVGNDKAHLHSTLEKSVAWVETNRPDAPIAAYSIEFL"
     gene            7530..8315
                     /locus_tag="PLC06_05900"
     CDS             7530..8315
                     /locus_tag="PLC06_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRO90014.1"
                     /translation="MLKEEVVESRPRSKAKAVLLGLLVLFFVMVAFLVFSIARLINTV
                     NDGATSAMEPIGDRMRSLFLPATPVILPNPATVVKQINDLARLETASYEMEKIVTADS
                     DQEGIMRVFLGESMIFVAYGKVYAGVDFARMQAADMVVVSPDTVKIHLPPAEIFADIP
                     VLDNERSFVADRDTGLLTPADPALETQVRQAAEQSIREAAQSSDILQRADLNAQAYMR
                     NFMAGLGFTEVIFTPDTPPATDAYQQVAPKGYYLLPTATPTAP"
     gene            8415..8825
                     /locus_tag="PLC06_05905"
     CDS             8415..8825
                     /locus_tag="PLC06_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90015.1"
                     /translation="MKIPLTIPYFRLVLGLTLAYGFLWMALEGELWRDLVLAAAVTLL
                     VTGYAMTRISGGRTLSVRHFVIVAVAAGLIAGAALSLITLFLMAIKMGIHAHGPEYAT
                     REIVWVWQQLPLWGAVGGLVGLGLGLLTAGKRIA"
     gene            complement(8822..9901)
                     /gene="hypE"
                     /locus_tag="PLC06_05910"
     CDS             complement(8822..9901)
                     /gene="hypE"
                     /locus_tag="PLC06_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRO90016.1"
                     /translation="MSNGQIPHLEGPVCPMPLRHDEKIVLGHGSGGKMSHDLIGRLFQ
                     RPFDNPALRAGNDAGVVSVPAGRLAISTDSHVVTPLFFPGGDIGRLAICGTVNDLAMM
                     GATPLYLTAGFILEEGLDVAVLARVVESMQAAAAEAGVTIVAGDTKVVQRGKADGLYI
                     NTAGVGIIDHDREIGGAQARPGDVVILSGPMGDHGIAVLAARGELGFAADVSSDVAPL
                     NRLVATLIAADASPARDGIHVLRDPTRGGVATTLNEIARQSGVAVVLDEVALPIRPSV
                     VAACEMLGFDPLYIANEGKLLAIVAPEEAKKVLAAMRATRYGEEAAIIGRIQATPAGR
                     VLLRTSIGSTRIVDMLAGEILPRIC"
     gene            complement(9918..11063)
                     /gene="hypD"
                     /locus_tag="PLC06_05915"
     CDS             complement(9918..11063)
                     /gene="hypD"
                     /locus_tag="PLC06_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRO90017.1"
                     /translation="MKYVTEYRDAELVKAAIREIGRAVTRPWVIMEICGGQTHAIVRH
                     GIDQLLPPEIELVHGPGCPVCVTPLELIDKALDIAGRPDVIFTSYGDMLRVPGSRGDL
                     FSVRATGGDVRVVYSPLDAVKIAQANPDKEIVFFAIGFETTAPANAMSVLQAKALGLT
                     NYSVLVSHVCVPPAMHAILGSPHNRVQGFLAAGHVNAVMGYWEYPPIAAKYHVPIVVT
                     GFEPLDVATGILAVVRQLESGRAEVENAYGRAVTRVGNTPAQQVIEQVFEVRDRQWRG
                     VGRIPASGWGLRPEFAGFDAEARFDVGEIRTQESPICIAGQILQGLKKPHDCPAFGTL
                     CTPESPLGATMVSTEGACAAYYRYGRHLENYGLQITNYELPEVVHSS"
     gene            complement(11060..11332)
                     /locus_tag="PLC06_05920"
     CDS             complement(11060..11332)
                     /locus_tag="PLC06_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRO90018.1"
                     /translation="MCLGVPGKITDIYETDGLRMGRIDFGGTQREACLAYVPEANVDD
                     YAVIHVGFAISLLSEEEAMATLETLREIADLEEELGPEVGIERERQ"
     gene            complement(11350..>11616)
                     /locus_tag="PLC06_05925"
     CDS             complement(11350..>11616)
                     /locus_tag="PLC06_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRO90019.1"
                     /translation="VDIGRISARFHNGVAQMTRDVCGHLRDEHGLNEIVLSGGVWQNA
                     TLLRRTLQLLQNDGFTVYTHHLVPANDGGLALGQAIVAMAQMPQ"
BASE COUNT         2122 a         3502 c         3768 g         2224 t
ORIGIN      
        1 cttcccggcg gtgacctgat catcgctgtg gatgggcggc cgatttcaag tcccgatgat
       61 ttgatcagct ttttggtctt caatgccgag gttgatcaga cgattatgct gacggtcatt
      121 cgtggcgggg agcaaataga ggtgcccctg acgctggctg aacgaccgta gcgtcgcgag
      181 cgcggcgggg caatttacat cgcgccggcg gctgcttcag tcttccggca atacaggcgg
      241 catattcagg taggaggttt tcatgtttag ccatgagcac atgcaagagc tggttaccta
      301 tgcaggaaat ggtcgcggcg ttgtcagtgt ctatctggat accgacaaca cccgacaatc
      361 catggaggcg attaagttaa ccgccagggg actcctcaag gaggttcagc cgcaatatga
      421 tcgggaagcg caggccatcg agcgttacct tgacctcagt tacgactggt cgaaacctgg
      481 gttggccttg ttttgcggca agggcgggga gttctttcgc gcctatgact ctccggtggc
      541 gtttcgcaat cgcgtccgcc tgagcacccg gccgtatgtc aagccattga cccatctgct
      601 cgatcattac gcccactttg gcgtgattgt ggttaatcag gtgggtggtg aattctacgc
      661 ttatcatttg ggcgaaatac aagccggcga catctatacc ggcgaggacg tgcgccgcat
      721 caaggacggc ggcggctcgt cggccgtggg gcgtcgcggc ggcagtggca gcggtgggcc
      781 gggcggcagc aaacatgagg acgaggtggc cgcgcgcaat atgcgcgaat gcgccgccgc
      841 cgccaatcaa ttcttttccg agtaccccat tcgccgcctc tttctggccg gcaccaatga
      901 aaacgtggcc caattccgcg agctgctccc caagcagttg cagagttgcc tggttggctc
      961 tttccccgta ggcaatgatg ccggcgagcc ggaagtacgg caacgcgcct tgaacctgct
     1021 gcgcgaagtc aacgccgagc gcgaggccaa gctcgtcgat cgcttgatca cgctcaacgc
     1081 gcagggaggc aacgctgtga tcggcctgga cgccgcactg caggcggtgt cggacaagcg
     1141 cgtggataca ctgatcatca gcgacggcta tcgcgcgccg ggttatttcc atgagccgtc
     1201 gggtttcgtg gttgccaacc tgactcgcag cccgctgact gaggcggatc tgacggaagc
     1261 ggccgacgtc atcgagacgg ctgtggccca gaccgtagcc cagggcggcc acgtcgaggt
     1321 catcgccgag aatccgtatc tcgaagacgc ggggcggatc ggcgctatct tgcgttacta
     1381 aactttgatt gaatgaagca ggcgtgccgt gcgttgaagg cgcggcacgc cttttttgtt
     1441 tccggcgtgg ctgaaagctg cgttatccgt cgcccggcgc gggtattttt tggcgagaac
     1501 tgggagccct tttggccgga tggcgaggtt aaggctccag cgccgagaag tagtcgcgga
     1561 cgagattttg caacccgatg ggtatgttcc cttgggagag agcctcaaag gctgcatccc
     1621 gataattgcc gaaaacctga tcatagggca cgaggctgcc gccgggtcgt ccctcgaggc
     1681 ttgacggcga ctgtcccgcc aacgggccgc aggtttcagg atcggtcagg cattgcacat
     1741 ccagttcaac cccttcgccc tctccttcaa gatcaggccg ctgagggacg aatacgctct
     1801 ccacgtggcc gccgcccggt gatgggccgc cggtgccgcc cgtctgggtt cctgtggatg
     1861 agccgctcga ttcacttgtt ccaccttgcc ccgtgccgcc ttctcctgca tcgccgctag
     1921 tgctgctttc tcccgtaccg gtgcccgtgc ctccgggctg ccctcctgcg ccgctctgag
     1981 ccacttcgcc gcgagcctgg cccagccggt cggccgtggc ggacgcctgc gcggcggctc
     2041 ccgcagtctg ggcgcgattg gccagctgcc cggccgcttc gtcgagcgct tcccgcgccg
     2101 tgccggtgtc gcccgcggcc agcgcttcgg ccgcgcgatc cagcgtctcc gccaattcag
     2161 cgtcggccga ttccagtgcg ttcgctgtag cggccagctg ttcggccagc gcggcctggg
     2221 cgccggcatc cagatcgccc aacgaagcag ccagtgcact cgtggcggcc gccgcctggt
     2281 ccagttgccc ggcgcgtagc gcctcggcca actcgcccgg ctgatccatt gcggccgcgg
     2341 ccaaggcctc gcgcagggac gccggatcca tctcctggct caaagccctc atctccgtct
     2401 cggccgcgga cagcgcggcc acggcctctt cgcgactgag gttgagccga ctcagggcgg
     2461 tcagcgcctc ctccagtggt tgggtcaacg cttcttgctg ttcggcggtc agggtatcgg
     2521 cggctttgat ttcatcgatc aattcggcga gcgcctgtga ctgctcgacg acggccgcgg
     2581 tcaccgcgcg ctgctccagt actaccgcct cttgcggatt aggcagccac agggccaggg
     2641 ccagcaaggc cactccggcg aaggcaggca gccaatcgga gagacgcgag cgcaggggta
     2701 actcacggtt cgcgtccacg gctgacgctg cccgcagtgc gtcatctaat tgccgcgtcg
     2761 ccatcgcgtc atcaaccgct agttgcccgg actgtatctc gatggcggcc gtcatgcgct
     2821 cacgcagatt gaaccggcgg tcggcgaagc gtgcctgatc gacgggagag cgtcgccgcg
     2881 ccatgacgat cagtccggtc acggagatgg cgatcagcgc cgcggccagg gtcaggagcg
     2941 ccagctctcc gcgcagcagt agcggccgcg cgcggctgag caacgccgcg accagcccca
     3001 agaacagccc gacggcaaga cccagcggaa ggtattgcag cagttcctga agccgccggc
     3061 ggccgtccca ctcgcgcagg atgcctgaca gacggtcata ttcgccggtc gtcatacgtc
     3121 gctaatccgc ctcacgcgcc gcacagtggc caagtagagg atgacagcgg ccagcacgtg
     3181 gaagataatg aacaaccacc agggggaaaa tatccacatc aattgtgtgc cgtgataggt
     3241 gtagccgagg agcgtatcct ggcttagcag gaatgcctcg ctaatgacca gagtggccgg
     3301 aagattcgtg gcagccagca caagccctac gtagatgaga atcatttcca gcgtggggcc
     3361 gggtgttcca atcgagaaac cggtgaagcc caacatcatg atggcgacga aggccagcat
     3421 agggatgccg aacgtcagga agagcgtccc cgcgaatgtc gctacggttg ccgtcagcgt
     3481 cgagcgcatg gctgccgaac accataaccc atagagggca tagggcgcgg cggtgattag
     3541 caccagcacc tgtgacagca ccagttccac cggcgacagc ccgcccaaca gaaaggcgag
     3601 actctgtaag gggatggaca cgatcaccag caggaagatg tagctgagcg ccgagacgag
     3661 ttttcccagc acgaaggcat tggccgggag cagcgtcgtg cgcagcagat cgaatgtctg
     3721 tcgctcgcgt tcgccgctga tcgatccggc ggtgaaggcc ggaccgataa aggtgaccag
     3781 gatgatctgc acgcccagca cggccgcgaa cagccccttg ccggttgtgc gggccatcgt
     3841 gttgctgggg acggcggccg aggacaggta gatcaggtag acgaagagga tcgccgcgct
     3901 gaggacgatc aggtagagcg tcagcacggc gaatgcccga tggccgcgca tccggctgcg
     3961 caattccttc gtcatcaccg gattgttaag cagcgcggtc cagggtcgtc gcgaggttgg
     4021 ggatggagtg gttgtttcag ccgtcatgag ttcgtgtttt atccgttcac tgtggacacg
     4081 ggcttcggct tattgggccg cggcttgcgt gacctgcagg aagacgtcct ccagatcgcc
     4141 gcgctcctcg ttgaagccga tcaccggcag gccatgggcg attgccccgg ccagcaggtc
     4201 gctcaatgcc tggtcgtcgc cgttgaagtc gagccgcatg cccgacgacg ggttgccgct
     4261 ctcgaccgtg gcggaaagca cacctggccg gccgcgcagc caattggcgg cctcattcgc
     4321 atcgaccgcc gcgccggaga caaaacgcat ggcgatcagc cggtggccgc gcaatcgagc
     4381 catcatctca tcgacgttgc cggtggccac aaggcggccg ttttcgatga tgccgatgct
     4441 ggtgcagatg tcggctactt cggccaggat gtgggacgag aagaagatcg tcttgcccat
     4501 cttctgtagc tcgcgcaaca gttcgcgcat ctcgatacgc gcccgcgggt cgagaccgct
     4561 ggccggttca tcgaggatga gcacctgcgg gtcgtgggtc agcgtgcggg ccaggcagag
     4621 gcgctgcttc atgccccggc tgagtgactc aacgtactcg tcgcgcttgt ggcccagatc
     4681 gaccagcgcc aacagatcgt cgatcatccc tggccgggtt ggcgcgggaa cgtcgtagca
     4741 ggcggcgaag aaatccagat attcccagac tttcatgtcc tcgtagacgc cgaagtaatc
     4801 gggcatatag ccgataaccc ggcgcacgtc acggggatcg tcagccaccg agtagccggc
     4861 gatgcgcgcc tcgcccgatg tcggccgcag gagggtcgtc aggatgcgca tggtcgtggt
     4921 cttgccggcc ccattcgggc cgatgaagcc gaagaccgcg ccttgatcga tcgccagatc
     4981 gagagaatcg acggccttca gatcgccata tcgtttcgtt aaatcgcgca gttcaatgat
     5041 cgccgtcatg gtcgattacc tgatgggtat ctccaatagc aggagggtcg tggccagcct
     5101 ctttgtcccc gcggccgatg atgtgacttc ctgcgcgctg ccggccagcc aaccgcccag
     5161 ggtaaccact ttggttgggt cgggcagggt ggtcgggtcg taatcctcgc ccatatagtg
     5221 agcctggatg agttgccagc gcggataggc taccggatcg ctgaagtagt cgtatgtgcc
     5281 cagaatgaga gacggatcgg tgatgagcgg gttggggttg atgaatccgg ccgaaaacat
     5341 cggatcgggc gttggtgatg ccgcggcaga ctcgccaaga gggaccgaca atgatttctc
     5401 ttcgccgggc gcgagattgc ccaaagttat ttgttcctgg ccgtggagga taaccgcgtt
     5461 ttccagggtg tcggccgtgc cattgcggac ggtgacgacg agtccactgc cgtcggccga
     5521 gcgcgtcgcc ccggcgctga tggccgggcg cggcagatgg gcgttgacga cgaaggtcgc
     5581 tatctggctg gtgtccgtgc ggacatcgcg caacagtacc tgacccgatc tttcgatcgc
     5641 ggcgagattg ccgccgctga cgagcgtccc gaatcccggc tggaagggga atgtcgatga
     5701 gtcatagggc agggtgaggt cataggtggt gcggcgcggc gaaaacaggc cgatgagcga
     5761 ttgggtttgc agccggtcgg cgtcggccga accgaaggcc accgacatga cgttcaatgt
     5821 ggcgtcattc ccccgcgcgc gaaaaccggt ggcgaaagtg atggcgctga acaggaggac
     5881 cagagccggg atggtgaccc aggccaattc gcggcgcctg gcgcggcgca aaatgatgta
     5941 attgatcggc ccgatcacga ggacataaac gagcagaaag aagaagagct gccaggccga
     6001 cggcagactc agccccggga tataggagat agcctgggac gccgcgtagg tatcctgaac
     6061 gccccagccc cacggcggca acttcggcgc gttggcgatg atcgccgtcc aaagcgcttc
     6121 gttgcccggc cggctggaga gcggagcgat cttcgggtcc aacgccagga aaaagacgct
     6181 cccacggccg atatcccgcc gcgccaggat tggcaggcca ttctgctcga tcaggacctc
     6241 gccggcgatg aggttgccgg tcgtggcgac gaatgggccg tcgccgctca ggggtgcgcc
     6301 ggcgaagtcg ccgagagcgg ccaggtcggc aagagattcc acgcccgtaa cggccacggg
     6361 cagcagttcg gctacaccgg cggccgtttt ggggccgccc ggtccgccgg tgacgacaag
     6421 ctggccgccg ctctcgatcc aggcgcgcag cgcggccagt tgcccggatg tcaggcggct
     6481 ggtgtcgatg tcgtcgagga caaggatgtc cagtgcgtcc caggcgctcg atacttcggg
     6541 caggtcatcg agcgtgagaa aggcgacggc cgcgtcgctc cggctaccct gaaccgtttc
     6601 caggaaggcc aacccgcccg gctcggggct aacgacgccg tagagcaggt cattccggcc
     6661 gagaccggtc aatcgattgg tctccacttc ggcgacgacg cgcccgcccg cgacgagttg
     6721 cgcggtcagc cggttgccga aagactccgt gtagacgacc agcggcacgc gcttgtccgc
     6781 gccagtgggt aatgtcagcg gggccgaata aaccagcgcg tttgcgccgc tcgcgccggg
     6841 ggagatgatt cgtatctcgc catcaaacgg ctcgcctcca ttgcgcgcgg tcacaacgac
     6901 ggggacggtc agcgacgttt catacaaacc gtcgaaaccg gcctgggcct cgagggtcag
     6961 gccgccgtcc tgcgcaaaag gcagcaggag tatgacaaag aggcgcgaaa gaagaaatga
     7021 cagaaacata tgttatagaa attcaatgct gtaggcggca atgggcgcat cggggcggtt
     7081 ggtctctacc caggcgaccg atttttccag cgtgctgtgg agatgggcct tgtcattgcc
     7141 gacgaccgcc agcccgatga cggccgtgcc ccaggcgtcc tggccgtcca cctcggccac
     7201 ggacaggttg aactcccgtt gcagccgggc gatgatcgat ttcacgatgc tgcgcttttc
     7261 cttgagagac cttacgccat ccagctccag cgtgatgatg cacgtgccca cgaacatgag
     7321 ttcaattgtc cgccaagagg gtatggatgc aaatgaggat catctgagtc ttatggacgg
     7381 ccttgagaca aatttaaggc agattacgta ttgcgttaag gttatatata cgttgatcga
     7441 ttatattgga aacataccca tgtatttttg atcaagggtt tttattttat tgtcgctcgt
     7501 atttattgac aggcactgaa ttatagacga tgttaaaaga agaagtggtt gagtcccggc
     7561 cgcgctcgaa ggccaaggcg gtcctgttag ggctgctcgt tctgttcttc gtgatggtgg
     7621 cttttctggt gttttcgata gctcgactga ttaatacggt caacgatgga gccacatcgg
     7681 cgatggagcc gattggtgac cgcatgcgct cgctcttcct gcccgccacg ccggtcattt
     7741 tgcccaatcc ggcgaccgtg gtgaagcaga tcaatgatct ggcccggctc gagaccgcct
     7801 cctatgagat ggagaagatc gtcacggccg actcggatca ggaagggatc atgcgcgtat
     7861 tcctgggcga gtcgatgatc ttcgtcgcct atggcaaggt ctacgccggg gtcgatttcg
     7921 cccggatgca ggcggccgat atggtcgtgg tctcgcccga cacggtgaag atccatctgc
     7981 caccggcgga aatcttcgcc gatatccccg tgcttgataa tgagcgctcg ttcgtggccg
     8041 accgcgacac cggcttgttg acgccggccg atccggcgct ggagacgcag gtgcgccagg
     8101 cggccgagca gtcgatccgc gaggcggccc agagcagcga tatcctgcag cgggccgatc
     8161 tgaacgcaca agcctatatg cgtaatttca tggccgggct ggggtttacg gaggtcatct
     8221 tcacccccga taccccgccg gcgaccgatg cttatcagca ggtagctccg aaggggtatt
     8281 atctgctgcc gacggcgaca ccgacggctc cctgatgagc gccgtataga gtacaataaa
     8341 acgggcgagt tcaatactcg cccgttttgt ttcatcggca tggttcccgc acagatggga
     8401 catgacctga gtctatgaag atccccctga caattccgta ttttaggctg gtgcttgggc
     8461 tgaccctggc ttatggcttc ctgtggatgg ccctggaagg ggaattgtgg cgggatcttg
     8521 ttctggctgc ggccgtaacc ttgcttgtga ccgggtatgc gatgacccgc atttcgggtg
     8581 ggcgaacgct atccgtccgg catttcgtga tcgtagctgt ggccgccggg cttatcgccg
     8641 gtgccgccct gtcgctcatc accctcttcc tgatggctat caagatgggt attcacgccc
     8701 acgggccgga atacgcgact cgggagatcg tctgggtgtg gcaacagcta cctctctggg
     8761 gcgcggtggg cgggctggtt ggcctcggtt tgggattgct gactgcgggc aagcggatag
     8821 cctagcagat gcgcggcaag atttcgcccg ccaacatatc cacgatgcgt gtgctgccga
     8881 tgcttgttcg caggagcacg cggccagccg gcgtggcctg aatgcgaccg atgatggccg
     8941 cttcttctcc gtagcgcgtg gcccgcatcg ccgccagcac tttctttgcc tcctccggcg
     9001 cgacgatcgc cagcagcttg ccttcgttgg cgatatagag cgggtcaaag cccagcattt
     9061 cgcaggcggc gactacggat ggcctaatag ggagcgctac ttcatccagg acgacggcta
     9121 cacccgattg tcgggcgatc tcgttcagag tggtggccac acctccccgc gtcggatcgc
     9181 gtagcacgtg gatgccgtct ctcgccggtg aggcgtccgc ggcgatgagt gtggcgacga
     9241 gccggttgag cggcgcgaca tcgctgctga cgtcggcggc gaagcccagt tcaccccgcg
     9301 cggccaggac ggcgataccg tggtcgccca tcgggccgga gaggatgacc acgtcccctg
     9361 gccgagcctg tgcgccgccg atctcgcggt cgtggtcgat aatgcccaca ccggcagtgt
     9421 tgatgtacag accgtcggcc ttgccgcgct gcacaacctt ggtatctccg gcgacgatcg
     9481 tcaccccggc ctcggccgcg gcggcctgca tcgactcgac aacccgcgcc agaacagcca
     9541 cgtccagccc ttcctcaagg ataaatccgg cggtcaggta gagcggagtc gcgcccatca
     9601 tcgccagatc gttgaccgtg ccgcatatgg ccagacggcc gatatccccg ccgggaaaga
     9661 acaacggagt gaccacgtgg gaatcggtgc tgatggccag ccggcctgcc gggacgctga
     9721 ccactccggc gtcgttgccg gcgcgcaatg cgggattgtc gaatgggcgc tgaaacaggc
     9781 ggccgatcag gtcgtggctc atcttgccgc cgctgccgtg gccgagcacg atcttctcgt
     9841 cgtgtcgcag cggcatgggg cataccgggc cttcaaggtg ggggatttgt ccgttgctca
     9901 tatgattgga tggcgaatca tgaagaatga accacttccg gtaattcgta attcgtaatt
     9961 tgtaatccgt aattttccag gtgtcttccg taacgatagt aggccgcgca ggccccctcg
    10021 gtggagacca tcgtcgcccc tagcggcgac tcaggcgtac acagcgtgcc gaaggccggg
    10081 cagtcgtggg gcttcttcaa gccttgcagg atttgaccgg cgatgcatat gggtgattcc
    10141 tgcgtgcgaa tttcgcccac gtcgaagcgc gcctcggcgt caaagccggc gaattccggc
    10201 cgcagccccc agccgctcgc cgggatccgg ccgacgccgc gccactgccg gtcgcggact
    10261 tcgaagacct gttcaattac ttgttgcgcc ggagtgttgc ccacgcgggt gacggcccgg
    10321 ccgtaggcgt tctcgacttc ggcccggccg gattcgagtt gtcgaaccac agccagaatg
    10381 ccggtggcga cgtcgagcgg ctcaaagccg gtgacgacga tcggcacatg atatttggcg
    10441 gcgatgggcg gatattccca gtagcccatc acggcgttga cgtgccccgc ggccaggaag
    10501 ccctgcaccc ggttgtgggg cgagccgagg atagcgtgca tggccggcgg tacgcagacg
    10561 tgggagacga ggacgctgta attggtcagg ccgagcgctt tggcctgcaa tacgctcatg
    10621 gcattggccg gggcggtcgt ctcgaagccg atggcgaaga agacgatctc cttgtcgggg
    10681 ttagcctggg cgatcttcac cgcgtcgagt ggcgaataga cgacgcgcac gtcgcccccg
    10741 gtggcgcgaa cggagaacag gtcgccgcgg ctgccgggga cgcgcagcat gtcaccgtag
    10801 gatgtgaaga tgacgtccgg tcgcccggcg atgtcgagcg ccttgtcgat cagctccagc
    10861 ggcgtgacgc acacagggca gccggggcca tgaaccagct cgatttcggg cggcagcagt
    10921 tggtcgatgc cgtgacggac gatggcatgg gtctggccgc cgcatatctc catgatgacc
    10981 catggccggg tgactgcgcg gccgatctcg cggatggcgg ccttcaccag ttcggcgtcg
    11041 cggtattcag tgacgtattt cattgccgct ccctctcgat ccccacttca gggccaagct
    11101 cttcttccag gtcggctatt tcgcgcagcg tctccagagt ggccatcgcc tcctcctcgc
    11161 tgagcaggct gatggcgaag ccgacgtgga taactgcgta gtcgtcgacg ttcgcttcgg
    11221 gcacataggc caggcaggcc tcgcgctgcg tgccgccgaa gtcaatgcga cccatgcgca
    11281 ggccgtctgt ttcgtaaatg tcggttattt tcccgggtac accaaggcac ataattgagt
    11341 atcctgtggt cattgaggca tttgcgccat cgctacgatc gcctgaccca aggccagccc
    11401 gccatcgttg gccgggacaa ggtgatgggt atagacggtg aagccatcat tctgtaacag
    11461 ttgcagtgtt cggcggagca gggtggcgtt ctgccacacg ccaccgctca atacgatctc
    11521 attcaaacca tgctcatcgc ggagatgccc gcagacgtcg cgggtcattt gggctacgcc
    11581 gttgtggaag cgagcgctga tgcggccgat gtcgac
//
LOCUS       DAOMQG010000071        11540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2407_length_11540_cov_10.353766, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000071 DAOMQG010000000
VERSION     DAOMQG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2407_length_11540_cov_10.353766"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLC06_05930"
     CDS             <1..710
                     /locus_tag="PLC06_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90020.1"
                     /translation="AYSPGDILAYDNATATWSIYFDASDVGVTKALDDFVLMNDGSIL
                     MTFKSRMKLLNGSGTQITIEPQDIARFVPSQLGDVTAGWFEQYFDGSDVGLTTSGEKI
                     DALAIRPDGTLLISSSGAATVKSGSVNVKAQDEDLLAFTLISSGATTSGTWHTTLALD
                     GSTLAGMAVENVTAAWADPDSQTIYLAVTSNFTIKGISGTNRTILSVSPTGDVATYWD
                     LSATGFNGQVDGLHIVR"
     gene            complement(717..2114)
                     /locus_tag="PLC06_05935"
     CDS             complement(717..2114)
                     /locus_tag="PLC06_05935"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HRO90021.1"
                     /translation="MTTLLAKNAQLLITMDDRRREIAGGGIFVRDNVIEAIGPGDTLP
                     DTADTVLDLSGHVVLPGLINTHHHLYQSLTRVIAQDADLFTWLKTLYPIWANLTDEAI
                     YVSTITGLAELALSGCTTSSDHLYIFPNDCTLDSQIRAAADIGVRFHAARGSMSLGES
                     QDGLPPDRVTEDEAFILRDSRRLIETYHDAARHAMTRVVLAPCSPFSVTADLMRESAA
                     LARAYGVRLHTHLAETIEEEAFCLETFGLRPVPYVESLGWTGDDVWHAHCVHMSAGEI
                     DLFGRTRTGVAHCPSSNMRLASGICHVLDLRRAGAPVGLGVDGSASNDSGHLLAEARQ
                     AMLLQRVAPDRYLSEAPGGRGGFAGNAGAMSAREALEIATRGSAAVLGRDDVGYLAPG
                     MSADFIAINLNRVDYAGAWHDPAAAVLFCGPRGVDWSVINGRVVVDNGRLTTVDLDAH
                     LPRHNAISRAMINGD"
     gene            2269..2350
                     /locus_tag="PLC06_05940"
     tRNA            2269..2350
                     /locus_tag="PLC06_05940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2303..2305,aa:Leu,seq:tag)
     gene            2410..3792
                     /gene="tig"
                     /locus_tag="PLC06_05945"
     CDS             2410..3792
                     /gene="tig"
                     /locus_tag="PLC06_05945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRO90022.1"
                     /translation="MTLTIQSSEDDLRQLTLTIEVDEDRVRKAMQKKARELSREVHLP
                     GFRPGKAPYDVLVRRIGEDTLRAEAVEDLVQPVFEEALEQEGVDLYAQPSLENIELHP
                     VTFKFIVPLSPTVVLGDYRAIRQDVEDVQVTDEALTEALEYVRMRHQVIEPVERAAEA
                     GDVVTIGGRGKFTAPKPAAEGEEAATEGEMLFEEESLDLLLDDKTLFPDTPFVENLIG
                     MSVGDQKEFTFVFPDPYEQDAEYAGREATFDVTVLAVKRRELPALDDELAKLEGKYET
                     LDELREALRDDLAKEAEAAIKEKTIDDMIHHLIEDATMVYPPAAVEAQIDDMLEDFKN
                     RLARSGWKYEDYLNLQGMTEENLRADFNENAEDQLRHQLALRQFILDEKLRVTAADVD
                     ELIDERIARFDNPGLRDSMRAYYRKGQGFDMISSEVLSNKAYERIRAIFSGNAPDLDA
                     LDEEAADEEE"
     gene            3908..4528
                     /locus_tag="PLC06_05950"
     CDS             3908..4528
                     /locus_tag="PLC06_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRO90023.1"
                     /translation="MFDIPTALVPMVVESTGRGERAYDIFSLLLKERIIILGTPINDQ
                     IANLTVAQLLWLASEDSQKPIRMYINSPGGQVYAGMAIYDTMQQVECPVSTVAVGFTA
                     SFGTILLAAGTKGMRYALPNATIHMHQPLGGARGQASDIAIQAQEILRLRSDLNQILS
                     AHTGQTIERIQKDTDRDLYMSATQAQEYGLVDQVLDSSKGTNGSGF"
     gene            4692..6536
                     /locus_tag="PLC06_05955"
     CDS             4692..6536
                     /locus_tag="PLC06_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRO90024.1"
                     /translation="MQNYMIKRFSRVQLWRKSPVVLLGAAILIAYLSAVSHPPAILAD
                     TNQLPTVGFTNNVYTVAENQGPAVITVSVTATPAAGQNVVVSYLTISGTATEGTGGDY
                     INTSGQLTFTNSSQNTQSFQVVINNDNIVNELSETVHLILQLQTPNTATIGVMQATLV
                     ITDDDTATATPRPTAATGTPIFVDLTEPNNTFEEAYQIQPDAPATCQLTLWPPGDKDY
                     YAFAVKAGTFYKIMTDQLSPGLDTVLKIYNQSGGPIAENDDIGSAGQFQSQVVIQSDT
                     TGFYYARVVNKSPADAANKTYCLAIDAMEQPTPTPSPTLLPTRIGSDPCEPNGTIPIA
                     CLFGENQSQAFNFVPPFNDGPDQDFYRIWVKPGVTITCETTELSSVTDTNMIFLGPNG
                     EDFNPQLGNDDRAPGDRSSILTYRSTYTGWLHVLVGPVNPVPLAQAAQFTYTLACTST
                     IPPTATPTTPPFTGGGGGVVVPIAPTITPFPTPTPIDLSAILTPPPVVIPEVTIQPMP
                     TATPPAGSQGVSTVNVTVFYDSNFNFTPEANEGIIDVAVALFDNTNGRLLAFGYTNQA
                     GAVRFDAVPSTGSVRVQVPLLNFSQIVGTGESNIAVRVAPLPLPIGIP"
     gene            6584..7966
                     /locus_tag="PLC06_05960"
     CDS             6584..7966
                     /locus_tag="PLC06_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90025.1"
                     /translation="MSADQQPPSGFGQRLTATPALLILGIVAIIALLAACIMGFFLLR
                     PLIPGSGGAATGGTAAPGEPTPFPQSTQSAAGQPVIIGVSDSGTISVTLDAPATLNVL
                     GTQWAVQPQSIGADGLWNPEVDDNAAAWVNGTIVNYVFGLKDSESNRTLLESLAPGQQ
                     MVVSTRQGVTHTFNFDSRNTVPSSDRSVFNQQIPGITLLLLGSNDDNRLVVRGRYEVA
                     PAEQTAGGGIIQMGESARLGDAQFTPQASSFVTNRPEAPVGFGFFLVDFVMQNVGLSA
                     IDTSAYRFTLSDELGNQYALNPIASRLGNFPPLTGFINAGQSLTATAGYQIPAGLISP
                     TLTWSITAPDGTHVQANLPFTAGGAAGQSAVISLQQATITSDLNNLIIVGQVTNVGVQ
                     PLVLAPDDVSLRTPAGADYLLLATNPPFPWTVPPNQTLQFAVTYQRPIGSSSAVFTIL
                     SQPFELSGLQ"
     gene            complement(7981..8460)
                     /locus_tag="PLC06_05965"
     CDS             complement(7981..8460)
                     /locus_tag="PLC06_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90026.1"
                     /translation="MSNKVLRIRRAVILGLLIIGSSLVIGAIAAEIYDISPTPGFGVL
                     QTLAFLLGVTALTTAIYMHLRASRPADATSSLQASIGMRLSATGLVLAYVSGFADLIR
                     IGTHYGPEFDRPFIGPLQLGGLLLGLLVLVVGILLYTTSRGKREASSLEFILNGKKK"
     gene            complement(8531..8604)
                     /locus_tag="PLC06_05970"
     tRNA            complement(8531..8604)
                     /locus_tag="PLC06_05970"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8568..8570),aa:Pro,seq:ggg)
     gene            complement(8660..>9386)
                     /locus_tag="PLC06_05975"
     CDS             complement(8660..>9386)
                     /locus_tag="PLC06_05975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRO90027.1"
                     /translation="FLGRILPSIDPESPVHTLALVSVGYLLGNAAISVSPGALDALAE
                     AHIEATLFDVVAQQFVFVLAAFLGVGLFTRRNGAELNARLGLERPTWPQIWTGVRWML
                     FFVILQGCIGAAWVLFDAAGAEQTSSINQSLLGSFDTVQEWFLLAAAAGLGEELLFRG
                     ALQPVFGILPVSLIFAVSHVQYGLSPATLAVFLLSVVLGIIRKRSNTTVAILVHGGYN
                     FILGLLSLLALYMEPLMRQLPPP"
     assembly_gap    9387..9486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9487..9930)
                     /locus_tag="PLC06_05980"
     CDS             complement(<9487..9930)
                     /locus_tag="PLC06_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90028.1"
                     /translation="MRLRTQHQSLISLLAVVAALLSLAACGPGAQQAVPTAESAPAQD
                     AALPTLPPADASTEGYPAPVVVATPADPYPAGEQPAPAPTVDPAGYPPPEEVFQEPRF
                     RIDQPVPVSATEIAGQAPPDTPLAIVDISYNGAVLGVGRSDANGRF"
     gene            complement(10014..11480)
                     /locus_tag="PLC06_05985"
     CDS             complement(10014..11480)
                     /locus_tag="PLC06_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90029.1"
                     /translation="MFKKILLVMVVLVAVLVSVSLVAAQSQLPGSGWWSGQQIQNVGT
                     STASVSFQAYNSSGTSFSCGNKSVGPGASANFLTIDCAALPAGFVGSAVVSADQPIAA
                     VVNVVNMSTNFDGTGGRAAGLYRGTDGSNVATEIAFPLMKNNFFGRSTALYVQNASDQ
                     TNDITVTIRVGASTYTKSFTGVKPYAMVVVGPADTSPAMPNSSYGSAKAVGTRPLAGT
                     ALEYQTNVAVGNNLHAYTAFTPQDFAAKAYCPLARSNAYNLNTGINVQNVGASAQKIK
                     VTYSYTNLGQGSVQTKTVEKGPVNPNAGVSFFTWEDLPKGSLGSAVVEGMGGGNIAVI
                     VNDETFSLNPNRVTAYSCFPDNAATKTNKVVLPQYKEFFLGDTAGVQIQNIGNAAATN
                     IRVTYYPNGGGAPVTFKNSNPVQANASFNLYRVSNPLPGVTVVSGTPSTLLNGFGSVV
                     IEADQPVVAIANEEPGFTPGANKPASKQDAKQYEGFNQ"
BASE COUNT         2289 a         3462 c         3572 g         2117 t
ORIGIN      
        1 tggcctattc gccgggagac atcctggcct atgacaacgc caccgccaca tggtcgatat
       61 atttcgacgc ttccgatgtg ggtgttacca aggcacttga tgactttgtg ctgatgaatg
      121 acggctcgat cctcatgacc tttaaatccc ggatgaagct gctgaatgga agcgggacac
      181 aaattaccat tgagccgcaa gacatcgcgc gctttgtgcc gtcccaactt ggcgacgtaa
      241 ccgccggttg gttcgaacag tattttgacg gctctgatgt gggcctgaca acatcggggg
      301 agaagatcga cgcgctggcc atacgtcccg acggcacgtt gctcatcagc tcctcgggcg
      361 cggccaccgt caaaagcggc agtgtgaatg tgaaggccca ggatgaagac ctgttggcct
      421 tcacgctcat ctcatcgggc gcgacgacat caggcacgtg gcacaccacc ctggcgttgg
      481 atggttcgac gctggcgggt atggcagtgg aaaatgtaac ggccgcgtgg gccgatcccg
      541 acagccaaac catctatctg gcggtgacca gcaacttcac catcaagggc atctcaggca
      601 ccaaccggac aatcctgagc gtgtcgccca cgggagatgt ggcgacctat tgggaccttt
      661 ccgcgaccgg ttttaacggc caggtagatg gcctccacat cgttcggtga agccgcctaa
      721 tctccgttga tcatcgcccg cgagatggcg ttgtggcgcg gcaggtgggc atccagatcg
      781 acggtcgtca gccgcccgtt atcgacgacg acgcggccgt tgatgaccga ccagtcgacg
      841 ccgcgcggcc cgcagaacag cacggctgcc gccgggtcat gccatgctcc cgcatagtcg
      901 acgcggttca gattgatggc gatgaagtcg gcgctcatgc cgggagccag atagcctacg
      961 tcgtcacgcc ccagcacagc cgcgctgcct cgcgtggcga tttccaatgc ttcgcgggcc
     1021 gacatcgccc cggcgttgcc cgcgaaaccg ccccggccgc ccggcgcttc gctcagatag
     1081 cggtcgggcg ccacacgctg caggagcatc gcctgccgcg cctcggccag cagatggccg
     1141 gagtcgttgg aagcggagcc atccacgccc aaaccgaccg gcgcgcccgc ccgccgcagg
     1201 tcgagcacgt ggcagatgcc gctggcgagc cgcatgttgc tcgacggaca atgggccacg
     1261 ccggtgcgcg tgcggccgaa cagatcgatc tcgcccgcgc tcatatggac gcagtgggca
     1321 tgccacacgt cgtcgcccgt ccagccgagt gattccacgt agggaaccgg ccgcaggcca
     1381 aacgtctcca ggcagaacgc ctcttcctcg atggtctccg ccaggtgggt gtgcagccgg
     1441 acaccgtaag cgcgggccag cgcggccgac tcgcgcatca ggtcggcggt gacggagaac
     1501 ggggagcagg gggccagtac gacgcgggtc atggcatgac gggcggcgtc gtggtaggtc
     1561 tcgatgagtc gccggctgtc gcgcaggatg aacgcttcgt cttccgtcac ccggtcgggc
     1621 ggcagaccgt cctggctctc acccagcgac atggatccgc gggcggcgtg gaagcgcacg
     1681 ccgatgtcgg ccgcggcgcg tatctggctg tccagcgtgc aatcattggg gaagatgtag
     1741 aggtggtcgc tgctggtggt gcagcccgag agggccagtt cggccaggcc ggtgatggtg
     1801 ctgacgtaga tcgcctcgtc ggtcaggttg gcccagatag ggtagagggt cttgagccag
     1861 gtgaacaggt cggcatcctg agcgatgacg cgagtcaggc tctgatagag gtggtggtgg
     1921 gtgttgatga ggccgggcaa cacgacgtgg ccggacagat cgagcacggt atcggccgtg
     1981 tccggcagcg tatcgccggg gccaatggcc tcgatgacgt tgtcgcgcac gaagatgccg
     2041 ccaccggcga tctcgcgtcg ccggtcgtcc atggtgatga gtaattgagc gtttttggcc
     2101 aggagtgtgg tcatgtggga gtttctctaa ttattgtagg ggcgggtttg aaacccgtcc
     2161 tcttttcctg ggcgggttat aaacctgccc tacacagata atcaagggta gcacggcgcg
     2221 ggattttggc aatgaaggcg ggtgagctat aattaccgtt ggtctgtcgg ggatgtggcg
     2281 gaattggcag acgcgcatga cttagaatca tgtgccgcaa ggcgtgtggg ttcgagtccc
     2341 tccatcccca ttaccaaaat cccaaggcgt tgcgctgcgg caacgctttt ttattcaaga
     2401 ggtatcgctg tgaccctaac cattcaatcc agtgaagacg atctgcggca actcaccctc
     2461 accatcgagg tagacgaaga ccgcgtgcgc aaggccatgc aaaaaaaggc ccgcgaacta
     2521 tcgcgcgagg tgcatctgcc cggcttccgg cccggcaaag cgccctatga cgtgctggtt
     2581 cgccgcatcg gcgaagatac attgcgcgcc gaggccgtcg aagacctggt tcagcccgtt
     2641 ttcgaggaag cgctggagca agagggagta gacctctatg cccaaccgtc gctggagaac
     2701 atcgagctac atcccgtcac gttcaaattc atcgttcccc tgtcgcccac cgtcgtgctg
     2761 ggggactatc gcgcgattcg tcaggatgtc gaagacgtcc aggtgacgga tgaagctctg
     2821 accgaggccc tggaatacgt tcgcatgcgg catcaggtca tcgagccggt ggagcgcgcg
     2881 gccgaagcgg gagacgtggt caccatcggc ggccgcggca agttcaccgc ccccaaaccg
     2941 gcggccgagg gcgaagaggc ggccactgaa ggcgagatgc tgttcgagga ggagagcctc
     3001 gatctcctgc tcgacgacaa gacgctcttc cccgatacgc cctttgtcga gaacctcatc
     3061 ggcatgtcgg ttggcgatca gaaggagttc accttcgtct ttcccgatcc ctatgagcag
     3121 gacgccgaat atgccgggcg tgaggcgacc ttcgatgtga ccgtcctggc ggtcaagcga
     3181 cgcgaattgc cggcgctcga cgacgaactg gccaagcttg agggaaaata cgagacgctc
     3241 gacgaactgc gcgaggctct gcgcgacgat ttggccaagg aggccgaggc cgcgatcaag
     3301 gaaaagacga tcgacgacat gatccatcat ctgatcgagg atgcgacgat ggtctatcca
     3361 ccggcggccg tggaagcgca aatcgacgat atgcttgagg atttcaagaa tcgcctggcg
     3421 cgctccggct ggaaatatga agattatttg aatttgcagg gcatgaccga agagaacctg
     3481 cgcgccgact tcaatgagaa cgccgaggac cagttgcgtc atcagttagc gctgcgccag
     3541 ttcattctcg acgagaagtt gcgtgtgact gcggccgatg tcgatgaatt gatcgatgag
     3601 cgcatcgccc ggttcgacaa tcccggcctg cgcgacagca tgcgcgccta ctaccgcaag
     3661 gggcagggct tcgacatgat cagcagcgaa gtgctgagca acaaggccta tgagcgtatt
     3721 cgcgccatct tcagcggcaa cgcgcccgac ctggacgcgc ttgatgagga agcggccgac
     3781 gaggaagaat aaaacagaaa tctaacgggc gctgtttata atgatgtaaa cagcgcccga
     3841 tgaggcgctt tgccgtcatc cgccccggcc tctttgccga gggcggtcag attgaggaga
     3901 agcagctatg tttgatatcc caacggcgct cgttccgatg gtggtcgaat ccactggccg
     3961 gggagagcgc gcctatgaca ttttctcgct tctgttaaag gagcgtatca tcattttggg
     4021 gacgccaatc aacgatcaga tcgccaatct gacggtggcg cagttgttgt ggctggccag
     4081 cgaggatagc cagaagccga ttcgcatgta catcaactcg cccggcggcc aggtgtatgc
     4141 cgggatggcc atctacgaca ccatgcagca ggtggaatgc cccgtctcca cggtggcggt
     4201 cgggttcacc gccagcttcg gcacgatcct gttggccgcc ggcaccaagg ggatgcgcta
     4261 cgccttgccg aacgccacca tccacatgca tcagccgttg ggcggcgcgc gcggtcaggc
     4321 gtcggacatc gccatccagg cccaggagat cctgcgcctg cgcagcgatt tgaaccagat
     4381 actgagcgcc cacaccggcc agacgattga gcgcatccag aaggacaccg accgcgatct
     4441 gtatatgtcg gcgacccagg cccaggagta tggcctggtg gatcaggtcc tcgacagctc
     4501 aaagggaacg aacggcagcg ggttctagcc catttcgcgg tcttgccgca gtgaaaaccc
     4561 catgacagga cagccggaaa ttaagaaatc cggctgtttt tttgtgcggg gcgggtcgtg
     4621 atttgcccgt caaccggtgt agacctataa tcaggccgat cgcgtggtga atatcatcag
     4681 gcgggagatg aatgcaaaac tacatgatta aacgtttctc gcgcgtgcag ctttggcgga
     4741 aatctccagt cgtccttttg ggagcggcga ttttaatcgc ttacctgagt gcggtttccc
     4801 atccccctgc aattttggcc gatacgaatc agctgccgac cgtcggcttc accaacaacg
     4861 tctatacggt agccgaaaac caaggtccgg cggtgatcac ggttagcgtc acggccaccc
     4921 cggctgcggg ccaaaatgtg gtcgtctctt acctgacgat atccggaacg gcgacagaag
     4981 gaaccggtgg cgattacatc aatacatcgg gacaattgac gttcaccaat tcatcccaga
     5041 atacgcaatc ctttcaggtt gtgatcaaca acgacaacat cgtcaatgag ttgtccgaaa
     5101 ccgtccatct catcctgcaa ttgcaaacgc cgaatacggc cacaatcggt gtgatgcagg
     5161 ctaccctggt catcaccgat gatgatacgg ccacggccac tccgcgcccc acggccgcca
     5221 cgggcacgcc gatcttcgtc gacctgaccg agccgaataa caccttcgaa gaggcctatc
     5281 agattcagcc cgacgccccg gccacttgtc agctcacgct ttggccgccg ggagacaagg
     5341 attactacgc gttcgccgta aaggcgggca cattctacaa aatcatgacc gaccagttgt
     5401 cgccggggct ggacacggtg ctgaagatct ataaccagag cggtggcccc atcgccgaaa
     5461 acgatgacat cgggtcggcc ggccagttcc agtcacaggt cgtcatccag tcggacacga
     5521 caggttttta ttacgcccgc gtcgtcaata aaagcccggc cgacgccgcc aacaagacct
     5581 attgcctggc gattgacgct atggagcagc cgacgcccac ccccagcccc acgctgctgc
     5641 ccacccgcat cggctctgac ccttgcgagc caaacgggac gataccgatc gcctgcctgt
     5701 tcggcgagaa tcagtcgcag gcgtttaact tcgtgcctcc gttcaacgat ggccccgatc
     5761 aggatttcta tcgcatctgg gtgaagccgg gggtgaccat cacctgcgag acgaccgagc
     5821 tttccagcgt gacggacacc aacatgatct tcctcggccc caacggtgag gacttcaacc
     5881 cgcagttggg caacgacgac agggctcccg gagacaggag cagcatcctg acctatcgat
     5941 caacctacac cggttggctc cacgttctgg tggggccggt caacccggtg ccgctggctc
     6001 aggccgcgca gttcacctat acgctggcct gcacatccac cataccgccg acggcgaccc
     6061 ctaccacacc gccgttcacc ggcgggggtg gcggtgtcgt ggttcccatt gcgccgacca
     6121 tcacgccctt cccgacgccg acgccgatcg atctgtcggc catcctgacg ccgccaccgg
     6181 tagtcatacc cgaagtgacc atccagccga tgcccacggc aacaccgccc gccggctcgc
     6241 agggcgtgtc cacggtcaac gtcaccgttt tttacgacag caactttaac ttcacgcccg
     6301 aggccaacga aggcatcatc gatgtcgcgg tggccttgtt cgacaacacc aacggccgcc
     6361 tgctggcctt tggctacacc aatcaggccg gcgcggtgcg tttcgatgcc gtgcccagca
     6421 ccggctccgt acgggtacag gtgccgttgc tgaactttag ccagatcgtc gggacgggtg
     6481 aatccaacat tgccgtgcgc gtggcgcctc tgccattgcc catcggtatt ccataaagga
     6541 gcggaaccac aagcacgctc caaacgtctt gtagcacaga actatgtcgg ccgatcagca
     6601 gcccccatcc ggtttcggac agcgacttac cgccacgccc gccctgctca tcctgggaat
     6661 tgtggcgatc atcgccttgt tggcagcctg catcatgggc tttttcctgc tgcggccgct
     6721 gattcccggc agcggcggcg cggcaaccgg cggcacggcc gcgcccggcg agccgacccc
     6781 cttcccgcaa tccacccaat cggccgcggg ccagcccgtc atcatcggcg tgagcgattc
     6841 ggggacgatc tccgtcactc tcgacgcgcc ggccacgctg aacgtgttgg gcacgcaatg
     6901 ggcggtacag ccgcagtcca tcggcgccga cggcctgtgg aaccccgaag tggacgataa
     6961 cgccgcggcg tgggtcaatg gcaccatcgt caactatgtc tttggcctga aggactcgga
     7021 gagcaatcga actttgctgg aatccctcgc gcccggtcag cagatggtgg tgtcgacgcg
     7081 ccagggggtg acccatacct tcaacttcga cagccgcaac accgtgccca gcagcgaccg
     7141 cagcgtgttc aatcagcaga tacccggcat caccctgctg ttgttgggca gcaatgatga
     7201 taatcgactg gtcgtgcgcg gccgatacga ggtggctccc gccgagcaga ccgccggcgg
     7261 cggcatcatc cagatgggcg aatcggcccg gctgggcgac gcgcaattca ccccgcaagc
     7321 cagctccttt gtgaccaatc gccccgaagc gccggtgggg tttgggttct tcctggtcga
     7381 ttttgtgatg cagaatgtgg gcctgtcggc catcgacacc agcgcttatc gcttcacctt
     7441 atcggatgag cttggcaatc agtatgcctt gaaccccatc gccagccgcc tgggtaattt
     7501 cccgccgctg accggtttca tcaacgccgg ccagtcgctg acggccaccg ccggctatca
     7561 gattccggca ggcctcatca gcccgaccct gacctggagc atcaccgccc ctgacgggac
     7621 gcatgtgcag gccaacctgc cgttcacggc cgggggcgca gcggggcaaa gcgcggtcat
     7681 ttcgctgcag caagcgacga tcacgtccga cctgaataac ctcatcatcg tggggcaggt
     7741 gacgaatgtc ggggtgcagc cgttggtgtt ggccccggat gatgtctcgt tgcgcacgcc
     7801 ggcgggggcg gactacctgc tgctggcgac caacccgccc ttcccctgga cggtgccgcc
     7861 aaatcagacg ctccagtttg ccgtcaccta tcaacggccg attggctcgt cgtcggccgt
     7921 cttcacgatt ctcagccagc cgtttgagct gagcggcctg caatagaccg cgctttccct
     7981 ctacttcttt ttgccgttga ggatgaattc cagcgacgag gcttctcgtt tgccccggct
     8041 cgtggtgtag aggagtatcc cgacgaccag caccaacaat cccagcaaca gaccccccaa
     8101 ttgcagcggg ccgatgaacg gccggtcgaa ctccgggccg taatgagtgc cgatgcggat
     8161 caggtcggcg aaaccgctga cgtaggccaa caccaggccg gtggccgaga ggcgcatccc
     8221 gatgctcgcc tgaagcgaag aggtggcgtc cgccggccgc gatgcgcgaa gatgcatgta
     8281 gatggccgtg gtgagggcgg tgacgcccaa cagaaaagcg agcgtttgca gtacaccgaa
     8341 gccgggagtg gggctgatat catagatctc ggccgctatc gcaccaatga ccagcgagct
     8401 accgatgatc agtaatccca atatgaccgc gcgccgaatg cggagtactt tattgctcac
     8461 ttgctatcca ttgggtgaat tggacggaaa acaaaagacg ctgcgccggg ttgacgcagc
     8521 gtcctgaaag tcggggcagc gggactcgaa cccacgacct cttcaacccc attgaagcgc
     8581 gctaccaagc tgcgccatgc cccgattgca ttgggaatta tatcaggctg ccggtaaatg
     8641 gcaaaccaca gactcgacat taaggcggcg gtaactgacg catcaacggc tccatataga
     8701 gggccagcaa gctcaacagc cccaggatga agttgtagcc gccgtggacg aggatggcca
     8761 cggtggtgtt gctgcgcttg cgaatgatgc ccagcaccac gctgagcagg aacacggcca
     8821 atgtcgccgg gcttagccca tattggacgt gggagacggc gaaaatgagc gaaacgggca
     8881 gaatgccgaa gacgggctgc aacgcgccgc gaaagagcag ttcttccccc aggcccgcgg
     8941 cggcggccag aagaaaccac tcctggaccg tgtcgaaact gccgagcaat gactggttga
     9001 tgctgctcgt ttgctcggcc ccggccgcgt cgaacagcac ccaggccgcg ccgatacagc
     9061 cttgcaggat gacgaagaag agcatccagc ggacgcccgt ccagatttgc ggccaggtcg
     9121 gccgctccag acccaggcgc gcgttgagtt ctgccccgtt tcgccgggtg aacaggccga
     9181 cacccagaaa tgcggccagc acgaagacga attgttgggc cacgacgtcg aagagcgtgg
     9241 cctctatgtg ggcttcggcc agtgcgtcga gagcgccggg agagacagaa atcgccgcat
     9301 tgcccagcag atagcccaca ctcaccagcg ccagggtgtg gacgggcgat tcggggtcga
     9361 tggacggcag gattcgcccc aggaacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnngaag cggccgtttg catcgctgcg tcccacgccc agcaccgcgc cgttatagga
     9541 gatgtcgacg atggccagcg gggtgtccgg cggggcctgg ccggcgatct cagtcgcgct
     9601 gaccggcacg ggctgatcga tacggaagcg cggctcctgg aagacctctt ccggcggagg
     9661 gtagccggca gggtcaacag ttggtgcggg ggccggttgt tcgccggccg ggtatgggtc
     9721 cgccggagtc gccacgacga ccggcgcggg atagccttcg gtggatgcat cggcgggcgg
     9781 cagagtcggc aaggctgcat cctgggcagg cgcgctttcg gccgtcggga ctgcctgttg
     9841 cgcgccaggc ccacaggcag ccagcgacaa taatgccgcc acgacggcga gcagggaaat
     9901 cagggattga tgttgagttc ttaatcgcat ggctaacatt ctacctgtat tgccgaaaaa
     9961 atagaagcca ccctaaggtg gcttctattc agcttcttaa tgtaagtcag ggcttactgg
    10021 ttgaaaccct cgtactgctt ggcatcctgc ttgctggcgg gcttgttggc gccgggggtg
    10081 aagccaggct cttcgttggc gatggccaca acgggctggt cagcttcgat gacgacgctg
    10141 ccgaatccgt tcaacagggt gctcggcgtg ccgctgacga cagtgacgcc gggaagcggg
    10201 ttcgaaacac gatagaggtt gaagctcgcg ttcgcctgaa cggggttcga gttcttgaag
    10261 gttaccggtg cgccgccgcc attcgggtag taggtcacgc gaatgttggt ggcggccgcg
    10321 ttgccgatgt tctgaatctg gacaccggcg gtgtcaccca ggaagaactc cttgtactgc
    10381 ggcagaacga ccttgttggt cttcgtggcg gcattatccg ggaagcagga gtaggcggtc
    10441 acgcggttgg gattcaggga gaaggtttcg tcgttgacga tgacagcgat gttgccaccg
    10501 cccatgcctt caaccacggc cgaaccgagg gagcccttcg gcagatcttc ccacgtgaag
    10561 aaggaaacgc cggcattggg gttcacaggg cctttctcga cggtcttggt ctggacggag
    10621 ccttgaccca gattggtgta ggagtaggtg accttaatct tctgcgccga cgcgccgacg
    10681 ttctggacgt tgatacccgt gttcagattg taggcgttgc tacgggccag ggggcaatag
    10741 gccttggccg cgaagtcctg gggcgtgaag gcggtgtagg catggagatt gttgccaacg
    10801 gcgacgttgg tctggtactc cagggcagtg ccggccagcg ggcgggtgcc gacggccttg
    10861 gcgctaccgt agctgctgtt cggcatggcc ggcgatgtat cggccggacc gacgacgacc
    10921 atagcgtacg gcttgacgcc ggtgaacgac ttggtgtagg tgctggcgcc gacgcgaatc
    10981 gtcacggtga tgtcgttggt ctggtcgctg gcgttctgga catagagcgc ggtcgaccgg
    11041 ccaaagaagt tgttcttcat cagcgggaag gcgatttcgg tggcgacatt gctgccgtcg
    11101 gtaccacgat acagacctgc ggcgcggccg ccggtaccgt caaaattggt ggacatattg
    11161 accacgttga cgaccgccgc gatgggctgg tcggcgctga cgacggcgga gccgacgaaa
    11221 ccggccggaa gcgcggcaca gtcgatggtc aggaagttgg ccgatgcgcc ggggccgacg
    11281 ctcttgttgc cgcaggaaaa cgacgtgccg ctgctgttat aggcctggaa tgagaccgag
    11341 gcagtgctcg tgccgacgtt ctggatctgc tggccggacc accagcccga accgggcaac
    11401 tggctctgcg ccgccacaag gctgacgctg accagtacgg cgaccaagac aaccattact
    11461 aacaggattt tcttgaacat gtaatatctc ctcacacaag ttcgctatgg ttagcagttt
    11521 acaatgggaa gtataggtgc
//
LOCUS       DAOMQG010000072        11465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2433_length_11465_cov_6.096231, whole genome shotgun sequence.
ACCESSION   DAOMQG010000072 DAOMQG010000000
VERSION     DAOMQG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2433_length_11465_cov_6.096231"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..979)
                     /locus_tag="PLC06_05990"
     CDS             complement(128..979)
                     /locus_tag="PLC06_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO90030.1"
                     /translation="MNTITMPPPRIGRVALAVADLDRSVRFYEDIIGLTLLERDGDSA
                     VLGAGDDGFLLLTEQPGARPVQRATGLYHFAILVPSRLDLARTLYHLLQNDAPISGFA
                     DHNVSEAIYLTDPDGHGIEIYRDRPREEWTYRNGQIAMSTDPIDLQGIINELRGRPQL
                     SSKMAPGTFAGHIHLQVAGIADTEAFYADLLGMDRLMRYGRQATFLSWNGYHHHVGAN
                     TWAGEGLAPAPEDAARLLWYEIVQPDEAAVESVAAHLSAAGHAAERDANDLWTVDPSG
                     IRVLLTH"
     assembly_gap    1199..1298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1299..1852)
                     /locus_tag="PLC06_05995"
     CDS             complement(<1299..1852)
                     /locus_tag="PLC06_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRO90031.1"
                     /translation="MTWQVDKSHTHINFLARHMMIAKVRGEFTDYDVNITFDENDLTA
                     TTVDVTIKADSVNTRDEKRDAHLMSPDFLNAAEYPVLTFKSKRVIQDGTNHGRLIGDL
                     TIRDVTHEVTLDVDYAGALKTPWGTTSAGFSGKATINRKDWGLNWNVALEAGGWLVSD
                     QVTIEIELELAQVPEVVAEAVAETA"
     gene            2006..2644
                     /locus_tag="PLC06_06000"
     CDS             2006..2644
                     /locus_tag="PLC06_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO90032.1"
                     /translation="MEILEIGTVGGGAITERHERADAAANRALILHTAERLFRERGVE
                     GVCMSEIAEAAGVGKGTLYRRYANKGELCLALMDTQMAEFQNAMLTLFNGLTAQGVPY
                     LEQLGAFLRSLVEFTENHSPLLVEVQRAGMLQSDERLNLPHFWQYMTVSALLRAAERN
                     GELKQGLDLEYLGEALLAPVQVGYFRFQRHARGYSVERIAAGLQSLVGRLAA"
     gene            complement(2667..4529)
                     /locus_tag="PLC06_06005"
     CDS             complement(2667..4529)
                     /locus_tag="PLC06_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90033.1"
                     /translation="MGGMMREKGRQWRVWAVLAALTGLLIMVGLAAGEGEKRLVPIGG
                     AYEEATLMLFAGQAAEHDSDGHVLIRVLPITYHRDAYSIGGRARRDNLALAQRRADQI
                     EEACTAVAAEGQTCEATAVDMMIHSDALDPAKVAELGPDVDGFYILGGDQTIAMLIIA
                     NTPAEAALTAGVADGAAVGGNSAGAAVISRYMISGYIGAAFAWHGLHQGAIDLWYGAD
                     DVAQRGLIFGQRQAVIDQHVLERGRTARLLQAMVEKPEDKLALGYDWGTGGVIDETGM
                     ARDVAGWYAAIALDGETWGAAANAQYVGDPAILAIHDVAWHVLPAGPYAYDLTTRRPV
                     VDGVAQDAPDISGRSFDHLKAPAGAGPLFIGGDISNDVRGEVLTSFVGAANDAGGPVI
                     VLATAPRHARALGYARFWRNRLRGLGLNARPEMITLTPDTDFAALGDRLAGAGAIFFT
                     GNDQALMAELVAGMEAGGAADALRAWWMDGGVLMADNAAAAALGVSMTAEPSPTGGNV
                     EYQSSDTFLDGYLTMAPGLGLVDAVIEPRVLYDYLYGRLVSHVMADPATVAVGVERGT
                     GLWVTSDGVSVIGREAAFVVDGRYAQTMAVGDNGAFAATWLLIDTFPTGTAMAE"
     gene            4714..6177
                     /locus_tag="PLC06_06010"
     CDS             4714..6177
                     /locus_tag="PLC06_06010"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90034.1"
                     /translation="MASFDYPKIKQLSANLAAGLLALWRDHRVAVPPAFATADRLATL
                     LEPGVAAFLGQLLMLAPDQADDLPAIIDRYFADPAMIDALDRFAARRVESAEELAGLL
                     FVAGLAPSLALHIYFRQALAMMFAAMTAALDRLSPDERPPFVVDGPLSLITFRYALDK
                     ALPLPDGVIAYEPIIDYFALEDMRGLGEAWRAQGLARVDLEGAIAADGLTVLFAWQPA
                     LMSGSPPPDEGTEAAMIGGDELIAELVEETTTAEPPGEATTTGPPGETMTPLRLDAAL
                     PDHVVVGRAFDLAVAIKRPEAPQLAPDDLDRRESAGFEAAWPADAAFIRLRVQISAPE
                     CLIHGGDSRDVRLPAGADGPPVYFQLTPQKAGPLSVIITVYQETDWVGSTRLRTEADN
                     EEPRGQLAMTVTSRPLGNGEVNLVQLRQALDDGYNDSEMRDLCFELNIDYEDLPGDGQ
                     SAKARELVLYARRHGLLAQLVEKVMQDRPQLLVTSDE"
     gene            complement(6252..7976)
                     /locus_tag="PLC06_06015"
     CDS             complement(6252..7976)
                     /locus_tag="PLC06_06015"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90035.1"
                     /translation="MVADTWLLMTLYWRLDRREVTTQSTLRILGFVLAAIGILFVSVF
                     SGFMGYGASFLTSSELSTPLPLGIVPGLLLTFVLLGVLVFGMNQALKALFMSGDLDRL
                     LVAPVHTRSVMVAKLLSRAPGNLLLLLLFAAPAFVAYGVGIGAGPIYYLLGGLMLLLA
                     PLFGLAVGAIIAMLLVRVLPVNRLNELLTAAYALVGIAIAILAQLPRLFLGGGDSGEF
                     AVEITEGSLGSAFTTLEKLPIPTFWAGRGLVALDAGQFELAGVLGILAYIVVTLGLFL
                     AVVMTADRLYLSGWLKTQTAGSKRQGLEESKGRFLNGSLAAAIARKDWLLRLRDPRQM
                     ISLLGGGFVAIVVGGLAIFRGGAGGDQSLMTLSQFGSSNETGFVAVFLAMFSPGVLMG
                     GWAMFVGSTFLMNTATYALALEGRSFSMLKAAPIRPREVWSAKVWSLYIPFVILFVVV
                     LLGTRFFVAYDLRWLPYALIAGLIIGYGLLAISVSTGFRYALLEWTDPRRMLSSGGGL
                     VSFLLSIAYGVPVAVIVLFGFALSAAWPAWTIPLALLALLLLAAIAFLCHVLMARWAD
                     SAWEKLPV"
     gene            complement(7966..8736)
                     /locus_tag="PLC06_06020"
     CDS             complement(7966..8736)
                     /locus_tag="PLC06_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019392312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90036.1"
                     /translation="MSEPVLVAENLTRLFGEFVAVDDAGFSLAPGEIVGFLGPNGAGK
                     TTTIKLLTGLLAPTSGKARIMGFDMATSPMEAKTRIGYAPDTPNLYGKLKAQEYLRFV
                     GQLYKVPPEKVEQRMRPMLDMFELTDVAGSLLDTFSHGMQQKVAVIGAFLHEPQIVFM
                     DEPTVGLDPRSARLIKDLMIRHRNGGGTIFFSTHILEIAQTMCDRVIIINKGRIVADA
                     RVEELRRMRGDQSLEDIFLELTGGSDVDEMVKELEDGR"
     gene            9090..9896
                     /locus_tag="PLC06_06025"
     CDS             9090..9896
                     /locus_tag="PLC06_06025"
                     /inference="COORDINATES: protein motif:HMM:NF040088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90037.1"
                     /translation="MPESPNGPAVNRSRLLEQIRALFSDGELRDLCLDLAVDYESLPG
                     EAKGDKARELILFMERLGRLDDLVDRATELRPHIGLNIKTLAEEIGHTDPARGAELTG
                     LVDQFRRYNERMVEWKELHRQINVLLTVFDPFRKQVERFDARPDRFDIYSLDANWQTV
                     NLFIGQLLRWATTIEHIGTPYRDSGGELSGVDWAVRLGTINGAIRRQLAARPARPNAN
                     PDDVWWQRLREETSNLEDAIKQVLILADEELRRTAADLFDLSAETLWRSA"
     gene            9893..11386
                     /locus_tag="PLC06_06030"
     CDS             9893..11386
                     /locus_tag="PLC06_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90038.1"
                     /translation="MTDFKTLQDAHLRLLTRQDAEPNSAAIVPDAQAYIAEVVAGSTW
                     IADPGERDLLRTYLRYWAGVIYERTGAYPKTELRPAESAAITPPTISPDPHVQPSPRP
                     EPPRRAPASGLWWLMPLLGLLVLLALAFAFVRFGRDITGEAINPSIPNPATALPQITR
                     TSLPPEVSLPATAIDGNNATTIQPLAQADAHTGGALAVTFDPARPEIATAGADGSVRF
                     WAWSAWKLTLSRQLDVGGGALNTVDYSPAPERALASTLFLTGGNNRILSVFNAESLQL
                     FADFVPSSPESGFVFAGRFSPDGALIAGGHADGVTRIWNVSTGTESANIATGTAARSR
                     LAQIPSGGPVRDVAFSPDGRYLAEASEAPEIGYQVIDRSWSNIVCEGRSGPAPAVAYS
                     PRGDLLAVGTVDGRLLFVAQTSNGCQLIEEQRAHEGSVTTVAFSPTGAWLVTGGRDGM
                     MRIWLPDGTLLAEIDVGAAVEAVAVAPDAAAIATADADGRLILWGIP"
BASE COUNT         2092 a         3865 c         3512 g         1896 t
ORIGIN      
        1 aatggaacga cgagcgcctc aagaacagct atgtgctgcc cgacgacgcg ctggcggaag
       61 tgcccagcga aatgagggag tagcgcgaca gtgggtcagt agatcagtag atcagtagat
      121 cagtgggtca gtgggtcagc aacacccgaa tccccgacgg gtccaccgtc cataagtcat
      181 tcgcgtcgcg ctcggccgcg tgaccggcgg cgctgagatg ggcagcgacg ctctcgacgg
      241 ccgcctcgtc gggctgcacg atctcatacc acagcagccg cgccgcgtct tcgggcgcgg
      301 gggcaaggcc ttcgcccgcc caggtgttgg cccccacatg atgatgatag ccgttccagg
      361 acagaaaggt cgcctgtcgt ccatagcgca tcagccggtc catgcccagc aggtcggcgt
      421 agaacgcctc cgtatccgcg atgccggcta cctgcaaatg aatatggccg gcgaacgtgc
      481 cgggggccat cttcgacgac aattgtggcc ggccccttag ctcattgatg atcccctgca
      541 gatcgatcgg gtcggtgctc atggcgatct gcccgttgcg gtacgtccac tcctcgcgcg
      601 gccgatcgcg gtagatctcg atgccgtggc cgtcggggtc ggtcagatag atcgcctcgc
      661 tcacattatg gtcggcaaag ccgctgatgg gcgcgtcgtt ttgcagcaga tggtagagcg
      721 tccgcgccag atcgagccgc gacggcacga gaatggcgaa atgatacagc cctgtcgccc
      781 gctgcaccgg tcttgctccc ggttgctccg tcaacagcag aaagccgtcg tcccccgccc
      841 ccaggacggc cgagtcgccg tcgcgttcca gcagcgttag cccgatgata tcctcataga
      901 agcgcaccga gcgatccaga tcggccactg ccagagcgac gcggccgatc cgtggcggcg
      961 gcatggtgat tgtgttcatg gcatttctcc tataacgcaa cgtccagacc gacctgacag
     1021 gtgggcgatc gatatcccgg ttggacgagc ggctgtgtcg cccacctgtc aggtcttcca
     1081 tagtatagcc cgaaaacaaa aaggacggcg gcccaatttg ggccgccgtc aaaaggagga
     1141 tgtgggagga gcctataccc taggcaaccg cttcggccac ggccgtctcg gccaccgcnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cgtctcggcc accgcttcag
     1321 cgacaacctc aggcacctga gccagctcca gctcgatctc gatggtcacc tggtcgctga
     1381 cgagccagcc gcccgcctcc agcgccacgt tccagttcag gccccaatcc ttgcgattga
     1441 tcgtggcctt gccggagaag cccgcgctcg tcgtgcccca cggcgtcttg agcgcgcccg
     1501 cataatcgac gtccagcgtc acctcatggg tcacgtcgcg aatagtcagg tcgccgatga
     1561 gacggccgtg attcgtgccg tcctggatca cgcgcttgct cttgaacgtc agaaccggat
     1621 actcggccgc gttcaggaaa tcgggcgaca tcagatgggc gtcgcgcttt tcatcgcggg
     1681 tgttgacgct gtcggctttg atggtgacgt cgacagtggt agcggtcagg tcgttctcgt
     1741 cgaaggtgat gttgacgtcg tagtcggtga actcgccgcg caccttggcg atcatcatgt
     1801 gccgcgcgag gaaattaatg tgggtgtgac ttttatctac ttgccaggtc attttgatat
     1861 atactccttt tcgttccggt cgacatattg attattatat ggacttgagt ccgtttattt
     1921 gttagaataa tagtggactt cagtccgctt gtcaagagaa atgcagcgga aaaccgaatc
     1981 ctaatttgag attaatatat tcgatatgga gatattagag ataggcacag tcggcggggg
     2041 agccataacc gagcggcacg aacgggccga cgcggccgcc aatcgcgcct tgatcctgca
     2101 cacggccgag cgcctctttc gcgagcgcgg cgtcgagggc gtgtgcatgt cggagatcgc
     2161 cgaggcggcc ggcgtgggca agggcacgct ctaccgccgc tacgccaaca agggcgagct
     2221 gtgtctggcg ctcatggaca cccagatggc cgagttccag aacgccatgc tgaccctctt
     2281 caacgggctg acggcccagg gcgtgcccta tctggaacaa ctgggagcgt ttttgcgctc
     2341 gctggtcgag ttcaccgaga atcattcgcc gctgctggtg gaagtccagc gagcggggat
     2401 gctccaatcc gacgaacggc tcaacctgcc ccacttttgg caatacatga ccgtcagcgc
     2461 gctgctgagg gcggccgaac gcaacgggga gctgaagcag ggcctcgatc tggaatatct
     2521 cggcgaggcc ctgctggctc ccgtgcaagt aggttatttc cgcttccagc gtcacgcgcg
     2581 cggctattcg gtcgagcgta ttgccgccgg gctgcagtcg ctcgtcggcc gcctggccgc
     2641 ctgaacgatg ctttgaccgc ttccgactat tcggccatcg ccgtgcccgt cgggaaggtg
     2701 tcgatgagca gccacgtggc cgcaaatgcg ccgttgtcgc ccacggccat cgtttgggca
     2761 tagcggccgt cgaccacaaa agccgcctcc cggccgatga ccgaaacgcc gtcggacgtc
     2821 acccacaacc ccgtgccgcg ctccaccccc acggccaccg tggccgggtc ggccatcaca
     2881 tggctgacca gccgcccgta gagataatca tagagtaccc gtggctcgat gaccgcgtcg
     2941 accagcccca ggccgggagc catcgtcaga tagccgtcga ggaaagtatc gctcgactga
     3001 tactccacgt tgccgcccgt aggggacggc tcggccgtca tgctcacgcc cagcgccgcc
     3061 gcggcggcat tgtcggccat cagcacgccg ccatccatcc accatgcccg cagcgcgtcg
     3121 gccgcgccgc ccgcctccat cccggctacc agctcggcca tgagcgcctg atcgttgccg
     3181 gtgaagaaaa tcgcccccgc gccggccagc cggtcgccca gagcggcgaa atcggtatcg
     3241 ggcgtcagag ttatcatctc cggccgcgca ttcagcccca ggccgcgcag ccgattgcgc
     3301 cagaagcgag catagcccag cgctcgcgcg tggcgcggcg ccgtcgccag cacgatgacc
     3361 ggcccgccgg cgtcgttggc cgcgccgacg aaggacgtca gcacctcgcc gcgcacgtcg
     3421 ttggatatgt cgccgccaat aaacagcggc ccggctccgg ccggagcctt cagatggtcg
     3481 aaactgcggc cgctgatatc gggcgcgtcc tgagccacac catccaccac cggccggcgc
     3541 gtggtcaaat cataggcata aggcccagcg ggcagcacat gccaggccac gtcgtggatc
     3601 gccaggatag ccggatcgcc cacgtattgg gcattggccg ccgcgcccca ggtctcgccg
     3661 tcgagggcga tggccgcata ccagccggcc acgtcacgcg ccatgcccgt ctcgtcgatg
     3721 acgccgcccg tgccccagtc atagccaagg gccagcttgt cttcgggctt ctcgaccatg
     3781 gcctgcaaca gacgcgcggt gcggccgcgc tccagcacgt gctggtcgat gaccgcctgc
     3841 cgctggccga agatgaggcc gcgctgggcc acgtcgtccg cgccatacca caggtcaatc
     3901 gcgccctgat gcaagccgtg ccaggcgaag gcagcgccga tatagccgga gatcatgtag
     3961 cgactgatga ccgctgcccc ggcgctgttg ccgcccacgg cggcgccgtc ggcaacaccg
     4021 gcggtcagcg ccgcttcggc cggcgtgttg gcgatgatca gcatggcgat ggtctgatcg
     4081 ccacccagga tatagaagcc gtctacatcc ggccccagtt cggccacctt ggccgggtcc
     4141 agggcgtcgc tgtggatcat catgtcgacg gccgtcgcct cgcaggtttg tccttcggcg
     4201 gctaccgccg tgcaggcttc ctcgatctga tcggcgcgac gctgagcgag ggccaggttg
     4261 tcgcgtcggg cgcggccgcc gatgctgtag gcgtcccggt gataagtaat cggcaacaca
     4321 cggatcaaca catggccgtc gctgtcgtgt tcggcggcct gccccgcgaa cagcatcagc
     4381 gtagcctcct catacgcccc accgatgggc acgagccgct tctcgccttc gcccgcagcc
     4441 agcccaacca taatcaataa ccccgtcaac gcggccagaa cggcccatac ccgccactgt
     4501 cgtcctttct cgcgcatcat gcctcccatc ttgtatttcc aaatttataa gccggccaca
     4561 aaacgtggcc aattgttggt cgtccgggtc tattatatcc gccaaacatt gatttttgga
     4621 ttacaatgat gcaatcgaaa ttcattgccc gaccggcaat ctgatccacc atcggcgacc
     4681 acaagttgag cacggccgtc acagaggcaa gccatggcat cattcgacta tccgaaaatc
     4741 aaacaactgt cggccaacct ggcggccggc ctgctcgcgc tgtggcgcga tcatcgcgtc
     4801 gccgtcccgc ccgcgttcgc cacggccgat cggttggcga cgctgctgga accgggcgtg
     4861 gccgccttcc tcggccaact cctaatgctg gcccccgatc aggcggacga cttgccggcc
     4921 atcatcgacc gctactttgc cgatccggcc atgatcgacg ccctcgaccg tttcgccgcc
     4981 cgccgcgtcg aatcggccga ggagttggcc gggctgctgt ttgtcgccgg tctcgcgccc
     5041 tcgttggccc tccatatcta ttttcgccag gccctggcca tgatgttcgc cgccatgacg
     5101 gccgcgctcg ataggctgtc gcccgacgaa cggccgccct tcgtcgtcga tgggccgctg
     5161 tcgctcatca ccttccgcta cgctcttgac aaggcgctcc ccctgcccga tggcgtaatc
     5221 gcctacgagc cgatcattga ttacttcgct ctggaagata tgcgggggtt gggcgaggcg
     5281 tggcgggcgc aagggctggc gcgggtcgat ctggaagggg ccatcgccgc cgacggcctc
     5341 accgtgctct tcgcgtggca gccggcgctg atgagcggct cgcctccacc cgacgaggga
     5401 accgaagcgg ccatgatcgg tggcgatgaa ttgatcgcgg aactggtgga agagacgacg
     5461 actgctgagc cacccggcga ggcgacgacc accgggccac ccggcgagac gatgacccct
     5521 ctgcggctcg atgcggcgct gccggatcat gtcgtcgtcg gccgtgcctt cgatctggcc
     5581 gtagccatca agcggccgga agcgccccaa ctggcccccg acgatctgga tcggcgcgag
     5641 agcgcgggct tcgaggcggc ctggccggcg gatgcggcgt tcatccggct gcgcgtccag
     5701 atcagcgcgc cggagtgcct catccacggg ggagacagcc gtgacgtgcg cctgccggcg
     5761 ggagccgacg ggccgccggt ctacttccag ctgacgccgc agaaagccgg accgctcagc
     5821 gtcatcatca ccgtctatca ggagacagat tgggtcggct ccacgcggct gcgcaccgag
     5881 gcggacaacg aggagccgcg cggccaactg gcgatgaccg tgaccagccg gccgctgggc
     5941 aacggcgagg tgaatctggt gcagctgcgc caggcgctcg acgacggcta caacgactcg
     6001 gagatgcgcg atctgtgctt cgagctgaat atcgactacg aggatttgcc cggcgacggc
     6061 cagagcgcca aggcccgcga actggtgctc tacgccaggc gccacggcct gctggctcag
     6121 ctggtggaga aggtcatgca ggatcggccg caacttttag tgacgagtga cgaatgacgg
     6181 gatacgaatg aagagcgccc cacgacgacg cgctcttcat tcgtatctcg tatctcgtat
     6241 ttcgtatttc gtcagaccgg cagcttctcc cacgcgctgt cggcccagcg ggccatcagc
     6301 acatggcaga ggaaggcaat ggcggccagc agcagcaggg ccagcagcgc cagcgggatc
     6361 gtccacgccg gccaggcggc cgacagcgca aagccgaaca ggacgatgac cgccacgggg
     6421 acgccatagg caatggagag caggaaggac accagcccgc cgccgctcga caacatgcgg
     6481 cgcgggtcgg tccattccag cagggcgtag cgaaaaccgg tcgatacgct gatcgccagc
     6541 aacccatagc cgatgatcag cccggcgatg agcgcgtagg gcagccaacg caggtcgtag
     6601 gccacgaaga agcgcgtccc caggagaacc accacgaaca ggatcacgaa cggaatatac
     6661 aggctccaca ccttggccga ccacacctcg cgcggccgaa tgggggccgc cttgagcatc
     6721 gagaacgacc ggccctccag cgccagcgca taggtggccg tgttcatcag gaaggttgac
     6781 cccacgaaca tcgcccaacc gcccatgagc acgccggggc tgaacatggc cagaaacacc
     6841 gccacgaaac cggtttcatt cgatgagccg aattgcgaca aggtcatcag gctctgatcg
     6901 ccgccggcac cgccgcggaa gatcgccagg ccaccgacga cgatggccac gaacccgccg
     6961 ccgagcaggc tgatcatctg ccgcggatcg cgcaggcgca gcagccagtc cttgcgggcg
     7021 atggcggcgg ccaatgagcc gttgagaaac cggcccttgc tttcctcaag tccctggcgc
     7081 ttgctgccgg cggtttgcgt cttcagccag ccggagagat agagccggtc ggcggtcatg
     7141 acgacggcca ggaacagccc cagagtgaca acgatatagg ccaggatgcc cagcacgccg
     7201 gccaactcaa attggcccgc gtcgagcgcc accaggccgc gcccggccca gaacgtgggg
     7261 atgggcaact tctcgagggt cgtgaaagcc gagcctaatg aaccctcggt gatctccacc
     7321 gcaaattctc ccgaatcgcc gccgccgagg aacaggcgcg gcaactgggc cagaatggcg
     7381 atggcgatac ccacgagggc gtaggcggcc gtcagcagct cgttcagcct gttcaccggc
     7441 agcacgcgca ccagcagcat ggcgatgatc gcgcccaccg ccagcccaaa caagggggcc
     7501 aggagcagca tcaggccgcc gagcagatag tagatcggcc ccgcgccgat gccgacgccg
     7561 taggccacga aggccggcgc ggcaaagagc aacagcagca gcaggttgcc gggcgcgcgg
     7621 ctcaacagct tggcgaccat caccgaccgg gtatgcaccg gcgcgaccag cagccgatcc
     7681 aggtcgccgc tcatgaacag ggccttcagc gcctggttca tcccgaagac cagcacaccg
     7741 agcaggacga aggtcagcag caggccgggc acgatgccca acggcagggg tgtcgatagc
     7801 tcggaagagg tcaggaatga cgcgccatag cccataaagc ccgaaaacac gctgacgaac
     7861 aggatgccga tcgccgccag gacgaagccg aggatgcgca gggtcgattg ggtcgtgacc
     7921 tcgcgccgat cgagccgcca ataaagcgtc atgagcagcc aggtatcagc gaccatcctc
     7981 aagctccttg accatctcgt ctacgtcgct gccgccggtc agctccagga agatgtcttc
     8041 cagcgactga tcgccgcgca tccggcgcag ctcctcaacg cgagcgtcgg ccacgatgcg
     8101 ccccttgttg atgatgatca cccggtcgca catcgtctgg gcgatctcca gaatgtgggt
     8161 ggagaagaag atcgtgccgc cgccgttgcg atggcggatc atcaagtcct tgatcagccg
     8221 ggccgagcgc ggatcgaggc cgacggtcgg ctcgtccatg aagacgatct gcggctcgtg
     8281 gagaaacgcg ccgatgacgg ccactttctg ctgcatgccg tggctgaacg tgtccagcaa
     8341 gctgccggcc acgtcggtca actcgaacat atcgagcatc ggccgcatgc gctgttccac
     8401 cttttccggc ggcactttat agagctggcc gacgaagcgc agatactcct gcgccttcag
     8461 cttgccgtag aggttgggcg tgtcgggcgc atagccgatg cgcgtcttgg cttccatggg
     8521 cgaggtggcc atatcaaagc ccatgatgcg agccttgccc gacgtggggg cgagcaggcc
     8581 ggtcaacagc ttgatggtgg tggtcttgcc ggcgccgttc ggccccagaa agccgacgat
     8641 ttcgcccggc gccagcgaga agccggcgtc atccacggcg acaaactcgc cgaatagcct
     8701 ggtcaggttt tcagccacaa gaaccggttc ggacattgga ttaactcctt gcacagttga
     8761 tctgttgttg cctattttat cttttttacg gattaaccgg aaataagttt cggcgagcgc
     8821 ggcagatgcc gtggggcaag ctgccaactt gcctcttcgc cccgctcgcc cgcaatacgg
     8881 cagattgcgc tacagtagtg gccggcaatc cggcagattg cgcttcagca atgggcggca
     8941 atctgacaga ttgcgctaca gcttcccccc ttctcccctt ctccctttct ccccttctcc
     9001 ctttctcccc ttcccccctt cttctccccc cgcttgacat ccccccatcc cgctgctacc
     9061 ctgatagcca tatagcaacg gaaccttcaa tgcccgaatc gcccaacgga cccgccgtca
     9121 atcgctcccg ccttctggag caaatccgcg ccctgtttag cgacggcgaa ctgcgcgacc
     9181 tgtgcctcga cctggccgtg gattacgaaa gcctgcccgg tgaggccaag ggagacaagg
     9241 cccgtgagct gatcctgttc atggagcggc tcggccgtct tgacgacctc gtggatcgcg
     9301 ccaccgaact gcgcccccac atcggcctca acatcaagac cctggccgag gagatcggcc
     9361 acaccgaccc ggcccgcggc gcggagctga ccggcctcgt cgaccagttc cgccgctaca
     9421 acgagcgcat ggtcgagtgg aaggaactcc accggcaaat caacgtcctg ctgaccgtgt
     9481 tcgacccctt ccgcaagcag gtcgagcggt tcgacgcccg acccgaccgc ttcgacatct
     9541 actccctcga cgccaattgg cagaccgtga atctgttcat cggccagctg ctgcgctggg
     9601 ccacgaccat cgaacatatc ggcaccccct atcgcgactc aggcggcgaa ctgtccggcg
     9661 tcgattgggc cgtgcgcctg gggacgatca acggcgccat ccgccgccaa ctggccgccc
     9721 gcccggcccg gcccaacgcc aaccccgatg acgtttggtg gcaacggcta cgcgaagaaa
     9781 cctcgaatct ggaagacgcc atcaagcagg tactcatcct ggccgacgaa gagctgcgcc
     9841 ggacggcggc cgacctgttc gacctgtcgg ccgaaacctt gtggagaagc gcatgaccga
     9901 cttcaagacc ctgcaagacg cccatctgcg gctgctgacc cggcaggacg ccgagccgaa
     9961 cagcgccgcc atcgtgcccg acgcccaggc ctacatcgcc gaggtcgtcg ccggcagcac
    10021 gtggatcgcc gaccccggcg agcgcgacct gctgcgcacc tatttgcgct attgggccgg
    10081 ggtcatctat gagcgcacgg gggcctatcc caagacggag ttgcgcccgg ccgaaagcgc
    10141 ggccatcacc ccgccaacga tttcccctga cccgcacgtc cagccgtcgc cccggcccga
    10201 accgccccga cgcgctccgg cgagcgggct gtggtggctc atgccgttgc tggggctgct
    10261 ggtgctgttg gccctggcat tcgccttcgt ccgcttcggc cgcgacatca ccggcgaggc
    10321 catcaatccg tccatcccca acccggccac cgccctgccg cagatcaccc gcacatcact
    10381 tcctccggaa gtatccctgc cggccacggc gatcgacggg aataacgcca ccaccatcca
    10441 gccgctggcg caggccgacg cccacaccgg tggcgcgctg gccgtcacgt ttgatccggc
    10501 acggccggag atcgccaccg ccggggccga cggcagcgta cgcttctggg cctggtcggc
    10561 gtggaaactg acgctcagcc gccaactgga tgtgggcggc ggggcgctca acacggtcga
    10621 ttactccccc gctccggagc gcgcgctcgc ctccacgctc ttcctcaccg gcggcaacaa
    10681 ccgcatcctc agcgtgttca acgccgaatc gctgcaactg ttcgccgatt tcgtgccctc
    10741 cagcccggag agcggcttcg tcttcgccgg gcgcttcagc cccgacggcg cgctcatcgc
    10801 cggcggccat gccgacggcg tgacgcgcat ctggaacgtc agcaccggca cagagagcgc
    10861 caatatcgcc accggcacgg ccgcgaggag caggctggcg cagattccat ccggtggacc
    10921 ggtgcgcgat gtggccttca gccccgacgg ccgctatctg gccgaagcgt cggaggcccc
    10981 ggagatcggc tatcaggtca tcgaccgttc gtggagcaat atcgtctgcg aaggacgctc
    11041 cggcccggcg ccggcggtgg cctattcgcc gcgcggcgac ctgctggccg tcggcacggt
    11101 ggacggccgc ctgctgttcg tcgcccagac gagcaacggc tgccaattga ttgaagaaca
    11161 gcgtgcccac gaagggagcg tgactaccgt cgccttcagc ccaaccggcg cgtggctggt
    11221 gacgggcggc cgcgacggca tgatgcggat ttggttgccc gacgggacct tgctggccga
    11281 gatagacgtg ggcgcggccg tggaggcggt ggccgtggct cccgatgcgg cggcgattgc
    11341 gacggccgat gccgatgggc ggcttatttt atggggcata ccgtgatata ctcccttcca
    11401 taacctcaac ggaaaccggg cgcatgacaa cctgctcaca ctgtggcgca aacaaccggg
    11461 acgac
//
LOCUS       DAOMQG010000073        11463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2434_length_11463_cov_11.587044, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000073 DAOMQG010000000
VERSION     DAOMQG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11463
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2434_length_11463_cov_11.587044"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLC06_06035"
     CDS             complement(<1..262)
                     /locus_tag="PLC06_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO90039.1"
                     /translation="MKVLVTGGAGYIGSITVERLIKHGASVVVFDNLYQGHEAAVHPE
                     ATFVRGDLADKAAIDDTLARHKPDGIMHFASYTLVGESWEKPF"
     gene            complement(361..915)
                     /locus_tag="PLC06_06040"
     CDS             complement(361..915)
                     /locus_tag="PLC06_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRO90040.1"
                     /translation="MKHPAIFLDRDGVIIENRSDYVRSWADVEIFPQAIDALVALCDS
                     PYRIVLVTNQSAVGRGIITRETAEGINDRLLAVIHEAGGRVDAVFMCPHGPDDGCDCR
                     KPLPGLLLRAAVELDIDLSRSLMIGDALTDIQAGQAAGVVSVLLRTGRGRDQEQLPAV
                     ATLSPFPIFDTLADALNSLIKSLP"
     gene            complement(991..1575)
                     /locus_tag="PLC06_06045"
     CDS             complement(991..1575)
                     /locus_tag="PLC06_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRO90041.1"
                     /translation="MLKTIDYDKTVTTEWYFDEMKAVLDRIDREPIYAAIDTLHQARM
                     DGKQVFIMGNGGSASTASHMVCDLAKNTRHSDWPHYKVIGMADNMAILSAYGNDDGYD
                     NVFRLQLANLLNPGDVVVAISTSGNSPNVLRAVELANERGATVIGMTGFGAGKLGPMV
                     DIHLHVPSDCIEQVEDIHLMLEHLITKALREMTR"
     gene            complement(1674..2654)
                     /locus_tag="PLC06_06050"
     CDS             complement(1674..2654)
                     /locus_tag="PLC06_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HRO90042.1"
                     /translation="MIIVQTPLRTSLFGGGTDFPGYYLEHGGCVLTSAIDKYIFVTIK
                     RRFDDKLRVGYTHTELVDDVDEIKHELIREALRQTGIDRGIEITTMGDIPSEGSGLGS
                     SSTVTVGALHAMYALNRELIMAEQLAKEACRIEIDVLGKPIGVQDQYIAAYGGLRFME
                     FMPGSGEVRSTRINLDPATKRQLNHNLLLFYTGVTRKADTILSEQQANIRSRESVLAE
                     MKQIAHTACDKLQQGDVDAIGDLLHESWELKKQLASKISNSDVDDLYAIARKAGATGG
                     KIAGAGGGGFLLLYCPPGKQEHLRQEMHMLQELPFNLGQDGSKVIFDYQR"
     gene            complement(2746..3621)
                     /locus_tag="PLC06_06055"
     CDS             complement(2746..3621)
                     /locus_tag="PLC06_06055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRO90043.1"
                     /translation="MTSITPRVTIGLPVYNGQNYLVETLESLLAQTFADFELVISDNA
                     STDRTEAICREYAARDARIHYYRNDENIGASANYNRAFELGRSEYFKWAAHDDVLAPT
                     YLERCVAVLDADPDVVLAYTQAKAIDSKGNIVKIYPGKQHFNSPDPRERFYEFVLDPH
                     PVVAVFGLMRREVLGRTRLIGKYAGSDRPLLSEMSLLGKFYEVPEHLFFYRFHEEQSW
                     GGNKSAQAQQAWYDPRRSGKITFPQWRLLREHLRSIERSPVGLKERVSCYLYMGYWMA
                     RNRKRLGQNLLLRDA"
     gene            complement(3629..4549)
                     /locus_tag="PLC06_06060"
     CDS             complement(3629..4549)
                     /locus_tag="PLC06_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRO90044.1"
                     /translation="MPYQLKSSDINLQTLSFGAFRVYKNAAQKLGVAGYDGNKTIVLI
                     LGIQRAGTSMMYWVFERDMGVKVYRESSELSSLDTGEHVRLNPLSAVIATIDRHHLPM
                     VVFKPLVESQRAHELMAAFPGSRVLWAYRHYQDVAASNLKAFGADNGHRDLQPFLAND
                     PNNWRSQNSSEETRQTIRRFYEMDLNPFDAAALFWWARMQLYFEQHLDQAPNVMLSRY
                     EDLVLDPARTMRRTYAFLGRPYPGDHIVKDVNAQSVGKGRVSRLSPAVDELCAAMLAR
                     LDHLNETSIRSLEGGVKPSILSSVTDDVRS"
     gene            complement(4571..5419)
                     /locus_tag="PLC06_06065"
     CDS             complement(4571..5419)
                     /locus_tag="PLC06_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRO90045.1"
                     /translation="MRVDFMVIGAQKAGTTSLAAQLAAHPQICFCKEKEPGFFHETED
                     WVGKLDEYHSLYHPMPGQLCGEASTYYTFYPEFRETHKRLYDYNPALKLIYVMRQPVE
                     RVISHYTHNRVREIDTRPPEEAVFSEGAYVNRSRYGVQLRPYLELFGPENVMLLVFEE
                     YTADQIATLYRIADFLGIDSAPFAETDTTPLHQSVGQPYLRSETARAFTQTGFFQKVR
                     GVVPASIRQPIRHRLLSNKIEEKPHFSPDVKQALWRLLEDDVHTVEQLLGRSLNIWRR
                     GYTDET"
     gene            complement(5437..6957)
                     /locus_tag="PLC06_06070"
     CDS             complement(5437..6957)
                     /locus_tag="PLC06_06070"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRO90046.1"
                     /translation="MELSSYFSILQRRKWIVILSTLLGLALAAAFTFLSTPQYVASTT
                     VRVLTVGGGTMTSARPDISYTERLVNTYSRIITGNKVRREIMDQLGLEKLPIISVQSI
                     VGTELIRVVAEATDPEDARDIANAAAQVLVDQNREFYSGGAGQTLQEILGEQLEQAEQ
                     ALNEARANHEKALEAQPPNEAAVAAAREALDVRDRTYASLLTQYEAARLEEAVRRNAI
                     SIIEEANTPNRPAKPRHVINLALGLAIGLVGGVALALLAENLDTTLYTAEQIESATQM
                     ETIGQIPTSKDDLAIARLGAGHYPQLESFRRLRTNILASGIDGSQVALLTSARRGEGK
                     STVAANLAVTIAQSGREVVVVDCDLRLPTVHKLFDIPNKRGLTNILAGEVQLDEAIQY
                     SSYPRVQVLTSGPLPPNPTELLGSQRMLDLIDQLKGQYDFIILDTPALLSVADAAVLA
                     PAVDNVILVVAQSQTRRGDVQTVRRQLANVRVKSMEVVVNRAEPNGSYAYYESEAR"
     gene            complement(7123..8406)
                     /locus_tag="PLC06_06075"
     CDS             complement(7123..8406)
                     /locus_tag="PLC06_06075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO90047.1"
                     /translation="MKILMINSFHYMRGGGERCFFDLSDLLRRHGHEVIPFCMGHPQN
                     FPSEYSDYFVGFIDFPTELSKPGLSPKLRVVERILYSREARRKVEAIIEDTRPDLVHI
                     HGFIHEMSTSILPALKAAGLPVVQTLHDYKIACPNTTFVSNDHICEACKGGRYYNMTR
                     YRCKRGSLSASLLASAEMYFHQMFRLYEPNIDMFISPSRFLADKMAEHGIRKPIIPIP
                     NFINPDNFTAVYEPQNHFVFAGRLVRVKGIMTLLEAMRHINSDAALMIAGAGELEPEM
                     RQFVETHNLTNVRFLGHLGTDDLAALVRSALFTVSPAEWYENYSMTVIESLACGTPVI
                     GANIGGIPEQVRDGWNGFLFEPGNATELAARMQSLLDDRELAIQMGRNGRAQVEAINN
                     PETHYEQTMQVYRRLLGDTAEHALANTANPTIDSI"
     gene            complement(8455..9576)
                     /locus_tag="PLC06_06080"
     CDS             complement(8455..9576)
                     /locus_tag="PLC06_06080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO90048.1"
                     /translation="MSSKKKIAFIGIKALPAKAGVDAVVQRIATSFDHERFEPTVYVS
                     SHEVPKETEISGVRLIRVPSIPGKFTRATVTFLMAALHALFLGDYDLVNIHSVETSFI
                     LPLLRLRYKVIATSHGLHSLVPKEYNQWGKGIYFFKACEYPFMHLANIRTSVSLPDAE
                     FLEKSYGREVKYLPIGIQLPDLKFDEAKAFLARHGLEPGNYMIFTAGRNIPRKGCHYV
                     LEAMQEIDDEMPLLILGDESFDPEYHRKLLSLADGRARFGGFVTDKGLLFALIQMANL
                     FLFPTTYEAMAATLLEAAALKTPLIASDLPENRAVLPNEALFFKSGDVDELREKMVWA
                     LGHPREMHELAGRAEAVVRSQFQWPRVIDQYEELYEALA"
     gene            complement(9650..10291)
                     /locus_tag="PLC06_06085"
     CDS             complement(9650..10291)
                     /locus_tag="PLC06_06085"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRO90049.1"
                     /translation="MKNPFKRNRNEKPALQPPAPIVIVSGLPRSGTSMMMKMLAAGGV
                     TPLTDELRAADGDNPKGYYEFERVKQLDKGDIGWLPMARGKAVKIISFLLQYLPADER
                     YQVIFLRRNLDEILASQRKMIINRGENPDQMDDAQVAAMFNRHIELVEKWLAQQPHID
                     TLYIHYSDVMADPVTQIESIAQFLGCELDRFAMAQVIDPDLYRNRQGPVLQAI"
     gene            complement(10298..>11463)
                     /locus_tag="PLC06_06090"
     CDS             complement(10298..>11463)
                     /locus_tag="PLC06_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRO90050.1"
                     /translation="YPAHHGVRAMVGARVSDSVPLLAELLRENGYHTYAEATGPLSEF
                     YRLNRGFDHYQYRDGVKSPMLGEWGDQLIERFRAGQLEEPWFAYMHLWGVHRPRQILP
                     EYDKPEYGRTQYDRAISSYDSRLRELFAAIDFENTVVLLTGDHGEKIPENNLENQVEF
                     FKKSLSGANKGTGRWARARRKAIASARATWFRTSRLLYRVGAVDSPLATVTGHGYHVY
                     DSLVRVPLVVSGGPTPVSRRVDSQVRQIDIMPTILDLAGLADSIPTTADGRSLLPMMH
                     GKALPELPALIETCQNSREPSSFYGVRYGGYKYAYDANDPNVPEELYDLTNDPEETKN
                     LAQAQPRKAVEMRGLIEAHIAQTETGAVNMTDEMSELEMAGLADHLRKLGYVE"
BASE COUNT         2543 a         3189 c         3231 g         2500 t
ORIGIN      
        1 ggaaaggctt ctcccacgac tcgcctacga gcgtgtagga cgcaaagtgc atgatgccgt
       61 cgggcttgtg gcgggccagt gtgtcgtcga tagccgcctt gtcggccaga tcgccgcgga
      121 cgaatgtcgc ctctggatgg acggccgctt cgtggccctg atagagatta tcgaaaacga
      181 cgacggatgc gccgtgcttg atgagacgct caaccgtgat actgccgata tagccggcgc
      241 cgccggtcac taatactttc atggcttgtc tatcctcatt taacttaaag tcagttgccg
      301 caaggtgatg cgccgttatg accagtttgt gaggatacac aagcaaggtt acggcatgtc
      361 ttacggcaaa gatttaatca gggagtttaa cgcgtccgcg agcgtatcga aaatggggaa
      421 tggtgacaga gtggccactg cgggcagctg ctcctgatcg cggccgcgac ctgtgcgcaa
      481 gaggacggaa accacgccgg cggcctgccc tgcctgaata tcggtcagag cgtcgccaat
      541 catcaacgaa cgactgaggt cgatatccag ctccacggcg gcgcgcagca gcaatccggg
      601 gagcggtttg cggcagtcgc agccgtcgtc gggaccatgg ggacacatga agaccgcatc
      661 gacccgtccc ccggcctcgt ggataacagc cagcagccgg tcgttgatgc cctcagctgt
      721 ctctcgcgtg ataatcccgc ggccgacggc cgactggttg gtgaccagca cgatccgata
      781 cgggctgtcg cacagcgcga caagcgcgtc gatggcttgc gggaagatct ccacgtcggc
      841 ccacgaacgc acatagtcac tgcgattctc aatgatgacc ccgtcgcggt cgagaaagat
      901 ggccgggtgt ttcatgagcg tgtgaaaggg agaaggggag aaagggcgaa gggggagatc
      961 actctttctc ccttcgccct ttctcctctt ctatcgcgtc atctcgcgca gagccttggt
     1021 gatcaggtgc tccagcatca ggtgaatgtc ttctacctgc tcgatgcagt cgctgggcac
     1081 atgaagatga atatccacca tcggccccaa cttgcccgcg ccgaagccgg tcatgccgat
     1141 gaccgtcgcc ccacgttcgt tggccaactc taccgcccgc aatacgttgg gcgagttgcc
     1201 gctggtcgag atagcgacga cgacatcgcc ggggttcagc aggttggcca gctgcaggcg
     1261 gaagacgttg tcatagccgt catcgttgcc ataggccgaa aggatggcca tattgtcggc
     1321 cataccgatc accttgtagt ggggccaatc ggagtgacgc gtattcttgg ccagatcgca
     1381 aaccatatgg ctcgcggtgg acgcactgcc accgttgccc ataataaaga cttgcttacc
     1441 gtccatgcgc gcctgatgca gggtatcaat ggcggcataa atcggctcgc gatcgatacg
     1501 atcgagcacg gccttcattt cgtcgaaata ccattcggtg gtgactgtct tgtcgtagtc
     1561 aattgtcttt aacatgatca ttcatcctta tatagaagaa gaatccacag atttcgtata
     1621 ttttcaaagg aagtatggcg cctcatttag agcggcggca tcccttagcc ggttcatcgc
     1681 tgatagtcga aaatcacctt gctgccgtcc tggcccagat tgaagggaag ctcctgaagc
     1741 atgtgcattt cctggcgcag atgttcctgc ttgccgggcg gacaatagag cagcaggaac
     1801 ccaccgcctc ccgcgccggc gatcttgccg ccggttgcgc cggccttgcg ggcaatggca
     1861 tacaagtcat ccacatcgct attactaatc ttgctggcca attgcttctt tagttcccag
     1921 ctctcgtgca acagatcgcc aatcgcgtcg acatctccct gctggagttt gtcgcaggcc
     1981 gtgtgggcaa tttgcttcat ctcggcaagc actgactcgc gcgagcggat gttggcttgc
     2041 tgctccgaca ggatggtatc agccttgcgc gtaacaccgg tgtaaaacag caagaggtta
     2101 tgattcaact ggcgttttgt ggccggatcc agattgatgc gcgtgctgcg cacttcgccg
     2161 ctgccgggca tgaattccat gaagcgcagt ccgccataag cggcgatata ttgatcctga
     2221 acgccaatgg gcttgcccag cacatcaatt tcgatacggc aagcttcctt ggccaactgc
     2281 tcggccataa ttaactcgcg attgagcgcg tacatcgcat ggagcgcgcc aacggtgaca
     2341 gtgctcgatg accccagacc ggacccttca gacgggatat cacccatcgt ggtgatctca
     2401 atgccgcggt cgatgccggt ctgcctaagg gcctcgcgga tgagttcgtg tttaatctca
     2461 tctacgtcat cgacaagttc agtatgggta tagccgacgc gcagcttgtc gtcgaagcgg
     2521 cgcttaatgg tgacaaagat atatttatcg atggccgagg tcagaacaca accaccgtgt
     2581 tccaaataat acccagggaa atcggttcct ccaccgaaca aactggttcg gagtggcgtt
     2641 tgtacaataa tcataacgtg aactcctgaa ttgaaggatt gcggccgtgc gacacctcac
     2701 aggataatgc aggtgtcagg cgcctcgatg ccgggtgtta caacatcaag catcgcgcaa
     2761 cagcaaattt tgacccagtc gcttgcgatt cctggccatc cagtacccca tataaagata
     2821 gcaggaaacg cgctctttca ggccgactgg tgaacgttcg atagagcgga ggtgttcgcg
     2881 caacagccgc cactgtggaa aagtgatctt gccggaacgg cgcggatcat accaggcttg
     2941 ctgcgcctgc gccgatttgt tgccgcccca ggattgctct tcatgaaatc gatagaagaa
     3001 cagatgctca ggcacttcat aaaacttgcc cagaaggctc atttcgctca ggagcggccg
     3061 atctgacccg gcatatttgc cgataagccg cgtgcgaccg agtacctcac ggcgcatcag
     3121 gccaaacaca gctacaaccg gatgaggatc caacacaaac tcataaaatc gctcgcgcgg
     3181 atcaggcgaa ttgaagtgtt gcttgcctgg gtaaatcttg acgatattgc ccttgctgtc
     3241 gatggccttg gcttgggtgt aggcaagcac gacgtcaggg tcggcgtcca gcaccgcaac
     3301 gcaacgctcc agataagtcg gcgcaagcac gtcgtcatgg gccgcccact tgaaatattc
     3361 gctccgtccc aactcaaagg cacggttata gttagccgac gccccgatat tttcatcgtt
     3421 gcgatagtag tggatccgtg cgtcgcgagc ggcatactcg cggcagatcg cctctgtccg
     3481 gtccgtcgag gcgttgtcgg agatgaccag ctcgaaatcg gcaaaggttt gcgccagcaa
     3541 ggattccagc gtctcgacga gataattctg gccgttatag accggcaggc caatggtgac
     3601 gcgcggggtg atagaggtca tggttttgtc aggagcggac gtcgtcggtc acggacgaca
     3661 ggatagaggg cttgacgccg ccttccaacg agcggataga tgtttcattg agatgatcga
     3721 gccgagccag catggcagcg cacaactcgt ctaccgcggg actgagcctg gatacgcgcc
     3781 ccttgcccac cgactgggca ttaacgtcct tcacgatatg gtcgccgggg taggggcgac
     3841 ccaggaaagc gtaggtgcga cgcatggtac gcgccggatc aagcaccagg tcctcatagc
     3901 ggctgagcat gacgttcgga gcctgatcca aatgttgctc gaagtagagt tgcatgcgcg
     3961 cccaccagaa gagcgcggcc gcgtcgaatg gattcagatc catctcgtag aagcgtcgaa
     4021 tcgtttggcg cgtttcctca gagctgtttt gcgagcgcca gttgttcgga tcgttcgcca
     4081 ggaacggttg aagatcgcgg tggccgttat ccgcgccaaa agccttgaga ttcgacgcgg
     4141 ccacatcctg ataatgacga taggcccaca acaccctgga gccggggaag gcggccatca
     4201 actcatgtgc tcgctgcgat tcgacaaggg gcttgaagac caccatcggc aaatgatgtc
     4261 ggtcgatggt agcgatcacc gcggagagcg ggtttagtcg cacatgttcg ccggtatcca
     4321 gcgaggaaag ttcgctggat tcacggtaga ccttaacgcc catatcgcgc tcgaacaccc
     4381 aatacatcat actggtgccg gcgcgctgga tgccgagaat gagtacgatg gtcttattac
     4441 catcgtaacc ggcaacaccc aatttttgtg cggcgttctt atagactcga aacgcgccaa
     4501 acgacagcgt ctgcaagttg atatccgatg acttcagttg atatggcata gcttcatcat
     4561 tggacgagac tcatgtctcg tcggtgtagc cccgccgcca aatattgaga ctgcggccga
     4621 gcaactgctc gacagtatga acgtcatctt ccagcaatcg ccacaaggct tgtttgacgt
     4681 ccggcgaaaa gtggggcttc tcttcgatct tgttcgaaag caggcgatga cgaattggct
     4741 gccggattga cgcgggcacg acgccgcgaa ccttctggaa gaacccggtt tgggtgaatg
     4801 cgcgagccgt ctcggaacgc agatagggtt gacccaccga ttgatggagc ggggtggtat
     4861 ccgtctcggc gaacggcgcg ctatcaatgc ccaggaaatc ggcaatacga tacagggtcg
     4921 cgatctgatc ggccgtgtac tcctcaaaga ccagcaacat cacgttttcg gggccaaaca
     4981 gttccagata ggggcgaagc tgcacgccat agcggctgcg gttaacatag gctccctcgc
     5041 taaagacagc ctcttccggc ggtcgggtgt cgatctcgcg cacacgatta tgggtgtaat
     5101 gggagatgac acgctcaacg ggctggcgca tgacgtagat cagcttcagc gccggattat
     5161 agtcatacag ccgcttgtgc gtctcgcgaa actccggata gaaagtgtag tacgtggacg
     5221 cttcgccgca caactggccg ggcatgggat gatagaggct gtgatattca tccagcttcc
     5281 cgacccaatc ttccgtctca tggaagaaac ccggctcttt ttccttgcag aaacagatct
     5341 gggggtgagc cgccagctgt gccgcaaggc tggttgtgcc cgctttctgg gcaccgatga
     5401 ccataaaatc gacgcgcatg atattccctg accgctctaa cgggcctcgg actcgtagta
     5461 ggcgtagctg ccgttcggtt cggcacgatt gacaacaact tccatcgact tcacgcggac
     5521 attagccagc tggcgtcgaa cggtctgaac gtcaccgcga cgggtttgtg attgagcgac
     5581 gaccaggatt acattatcca cggccggagc cagcacggcc gcgtcggcca ccgacaacag
     5641 cgccggcgtg tcgagaatga tgaaatcata ttggcccttc aactggtcaa tcaggtccag
     5701 cattcgctgc gaaccgagca actctgttgg attaggtggc aaggggccac tagtgagtac
     5761 ttgcacgcgt ggataggaac tgtattgaat cgcctcatcc aactggactt caccagccag
     5821 gatattggtc aggccgcgct tgttggggat gtcgaacagc ttgtgaacgg tcggcaggcg
     5881 caggtcacaa tcgaccacaa ccacctcgcg accggattgc gcaatggtga cggccagatt
     5941 ggcggcgaca gttgatttac cttcgccacg cctggcgctg gtcagaaggg cgacttgcga
     6001 gccatcaatc cccgaagcca ggatattagt gcgcaatcgc ctaaaagatt caagttgcgg
     6061 ataatggccc gcccccaacc gggcaattgc cagatcatcc ttggaagtcg ggatttggcc
     6121 aatggtttcc atctgggttg cgctttcaat ttgctcggcc gtgtagaggg tcgtatccag
     6181 attctcggcc agcagcgcca gggcgacacc acccaccagg ccaatggcca agccgagagc
     6241 caaattgatc acgtgacgtg gcttggccgg gcgattggga gtgttggctt cctcgatgat
     6301 ggagatagca ttgcgccgaa ccgcctcttc gagccgggcg gcttcatact gcgtcagaag
     6361 tgaggcatag gtgcggtcgc gcacatcaag tgcctcgcgg gccgcggcca cggccgcttc
     6421 gttcggcggc tgcgcctcaa gcgccttttc atggttggca cgcgcctcgt ttagcgcctg
     6481 ttccgcctgc tccagctgct cgcccagaat ctcttgcaag gtctggccgg ccccgccgct
     6541 ataaaattcg cggttctggt cgaccaatac ctgtgctgcg gcattggcga tgtcgcgagc
     6601 gtcttcgggg tcagtcgcct cagccacaac gcgaatcagt tctgtgccga caattgattg
     6661 gaccgatatg atgggcaatt tttccaggcc aagctgatcc ataatttcgc gccgcacctt
     6721 attgccggtg atgatgcgcg aatatgtgtt gaccagacgc tcggtatagg agatgtcggg
     6781 ccgggccgac gtcatggtgc cgccaccaac agtcaacaca cgaaccgtag tcgaggcgac
     6841 gtactgcggc gttgaaagga aggtaaatgc ggccgcgagc gccaacccca ggagcgtgct
     6901 caggataact atccatttgc gccgctgaag aatcgaaaaa taagaactta actccatact
     6961 ctcttttgct cctgccaggg acttccaaac cagtgcccaa tgcgaacagt gctaggttat
     7021 caatcgatga ttacgatcaa ccttgcgacc gaatcgcttt catcatattg caaggatatc
     7081 agtgaagcgg gaattttact tcaaccgctc atgataatta cgttaaatcg agtcgattgt
     7141 tggattggct gtgttcgcca aggcatgttc cgctgtatcg cccaacaacc gtctataaac
     7201 ctgcatggtc tgctcgtagt gtgtttcagg gttgttgatg gcctctacct gagctcgtcc
     7261 attgcgtccc atttgaattg ccaattcacg gtcgtcaagc aaggattgca tcctggccgc
     7321 cagttcagtt gcattgcccg gctcaaacag aaatccattc cagccatcgc gcacctgctc
     7381 gggaatgccg ccaatattcg caccgataac cggcgtacca caggccagcg actcaatgac
     7441 tgtcatcgag tagttctcat accactctgc gggcgaaaca gtgaatagtg cactccgcac
     7501 cagggccgcc aaatcgtccg tcccaagatg acccaggaag cgtacattgg tcagattgtg
     7561 ggtttctaca aattgtcgca tctccggttc caattcgccg gcaccggcaa tcatcagggc
     7621 agcatcgcta ttgatgtgcc gcatagcctc aagcaaggtc atgatacctt tgacacgaac
     7681 caggcgcccg gcaaagacaa aatggttctg tggctcgtag acggccgtaa agttgtcggg
     7741 attaataaag ttagggatag ggatgatcgg cttgcgaatg ccgtgctcgg ccatcttgtc
     7801 ggccagaaat cggctgggtg aaatgaacat atcgatattg ggttcataga gccggaacat
     7861 ctggtggaaa tacatctcgg ccgaagccag caggctggcg ctaagcgagc cgcgcttaca
     7921 gcgatagcgc gtcatattat agtaacgccc acccttgcac gcctcgcaga tatgatcatt
     7981 ggagacgaag gtcgtgttgg ggcaggcaat tttgtaatca tggagcgttt ggacgaccgg
     8041 cagcccggca gccttcagcg cgggcagaat cgacgtagac atttcgtgaa tgaaaccatg
     8101 gatatggacc agatccggcc gcgtatcctc aatgatggct tctaccttgc gtcgtgcctc
     8161 ccgtgagtag aggatgcgtt cgacgacccg caacttcggg ctgaggcccg gcttgctcaa
     8221 ctcagtggga aagtcgatga aaccgacgaa gtaatcacta tattcggagg ggaagttctg
     8281 cggatggccc atgcagaacg ggatgacctc gtgaccatgg cggcgaagta aatcggatag
     8341 atcaaagaaa cagcgctccc cgccgccgcg catataatga aagctgttga tcatcaaaat
     8401 cttcatgata cgctgtttcc caaactatct gcagcagacc tggccggcgg catgctaagc
     8461 caaagcctca taaagctctt cgtattgatc gatgactcgc ggccattgga attgcgagcg
     8521 caccactgct tcggctcggc ctgcaagctc gtgcatctct cgtggatggc ccagcgccca
     8581 gaccatcttc tcgcgcagtt catcgacatc tcccgacttg aagaaaagcg cctcattggg
     8641 cagtacagcc cgattttcag gcagatcact ggcgatcagg ggcgtcttca atgcggccgc
     8701 ctccagaagt gtcgccgcca ttgcctcata agtagtcggg aaaaggaaca ggttggccat
     8761 ttgaatgagc gcaaaaagca ggcccttgtc ggttacaaag ccgccgaaac gagcgcgtcc
     8821 gtcggccagc gacaaaagct tccggtgata ctccgggtcg aaagactcat cacccaatat
     8881 gagcaacggc atttcatcat cgatttcctg catcgcttcc agaacgtaat ggcatccttt
     8941 acgcggaata tttcgaccgg cggtgaagat catatagttc cccggctcca gcccatggcg
     9001 agccagaaac gctttcgcct cgtcgaattt tagatcgggt aactgtatgc cgatcggcag
     9061 atatttcacc tcgcgaccat agctcttttc caaaaactcg gcgtcgggca aggatacgct
     9121 tgtgcggata ttggccagat gcatgaaagg gtattcgcag gccttgaaaa agtagatccc
     9181 cttaccccac tgattatatt ctttcggcac caggctatgt aaaccatgag atgtcgcaat
     9241 caccttatag cgcaagcgta atagcggcaa gatgaaactt gtctcgacgc tgtggatatt
     9301 aaccaggtca taatctccga gaaagagtgc atgaagtgca gccatgagga atgtcactgt
     9361 agctcgggtg aacttgccgg ggatactggg cacgcgaatt agacgcacgc cggagatttc
     9421 cgtttctttg gggacttcgt ggctgctgac gtagactgtc ggttcgaacc gctcatgatc
     9481 aaacgaagtg gcgatccttt gcacgacggc atcaacgccg gctttggcag gcaaagcctt
     9541 gattcctatg aaagcgatct tctttttgct ggacatattt ttatctgtac gcatgcggcg
     9601 tgagatggtc agtcgagacg acttgtatca gagtggcctg tccgtcactt taaatggcct
     9661 gtaataccgg cccttgccgg ttgcgataga ggtcggggtc tataacctgg gccatcgcga
     9721 accggtcaag ttcgcagcca aggaattggg cgatcgattc gatctgcgtc accgggtcgg
     9781 ccatgacgtc gctataatgg atatagagcg tgtcaatgtg gggctgttgc gccaaccatt
     9841 tctccaccag ttcgatatgc cggttgaaca tggccgccac ctgggcatcg tccatctgat
     9901 ccgggttctc accacgattg atgatcatct tgcgctgtga ggccaaaatc tcatcgaggt
     9961 tacgacgcag gaagatcacc tgatagcgtt cgtcggccgg caggtattgc agcaagaagg
    10021 agatgatttt tactgccttg ccccgcgcca tcggcagcca accgatatcg cctttatcca
    10081 gttgtttcac gcgctcaaat tcgtagtagc ccttggggtt atcgccgtcg gccgcgcgca
    10141 gctcgtctgt caatggggtg actccgccgg ccgccagcat cttcatcatc atcgaagtcc
    10201 ccgagcgtgg caagccggag acaatgacaa ttggcgcggg aggttggagt gccggtttct
    10261 cgttccggtt tcgtttgaat ggatttttca tgccgcccta ttctacgtag ccgagcttgc
    10321 gcaagtggtc ggccaggccc gccatctcca gctcgctcat ctcatcggtc atattcaccg
    10381 cgccggtttc ggtctgcgcg atatgggctt caataagccc gcgcatctca acggccttgc
    10441 ggggttgggc ctgggccaga ttcttcgtct cttccgggtc gttcgttaga tcatacagct
    10501 cttccggaac attggggtcg ttggcgtcat aggcgtactt atacccgccg tagcggactc
    10561 catagaatga ggaaggctcg cgagaatttt ggcaagtctc gatcagggcc ggcagttccg
    10621 gcagcgcctt gccatgcatc atcggcagca agctgcggcc gtcggccgtg gtggggatgc
    10681 tgtccgccag cccggccaaa tcaagaatcg tcggcataat atcgatctgt cgcacttggc
    10741 tgtctacgcg tcgggagaca ggcgtgggac cgccggagac aacgagcggc acacggacga
    10801 gggaatcata aacatgatag ccgtgcccgg tgacggtcgc cagcgggcta tcaaccgcac
    10861 caactcgata gagcaggcgc gaggtcctga accaggttgc tcgtgccgaa gcgatggcct
    10921 tgcgtctcgc ccgcgcccag cgccctgtgc ccttattggc acccgatagt gatttcttga
    10981 agaactccac ctgattttcc agattattct ccgggatctt ctcgccatga tcgccggtca
    11041 gcagaacgac cgtgttttca aaatcaatag cggcgaataa ttcacgcaag cgcgagtcat
    11101 agctggaaat ggcgcgatcg tactgtgtgc ggccgtattc gggcttgtca tactcaggga
    11161 gtatttgtcg cggccgatga accccccaga ggtgcatgta ggcaaaccag ggctcttcaa
    11221 gttggcccgc gcggaagcgc tcgataagtt gatcacccca ttcgcccagc ataggtgact
    11281 tcacaccatc gcgatactgg taatggtcaa agccgcggtt gagacgatag aactcgctca
    11341 gcgggccggt ggcttcagca taagtatgat aaccattctc acgcaagagt tcggccaaca
    11401 gcggcacact atcggaaaca cgcgcgccaa ccatggctcg cacaccatga tgggcgggat
    11461 aca
//
LOCUS       DAOMQG010000074        11437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2438_length_11437_cov_9.629415, whole genome shotgun sequence.
ACCESSION   DAOMQG010000074 DAOMQG010000000
VERSION     DAOMQG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2438_length_11437_cov_9.629415"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLC06_06095"
     CDS             complement(<1..614)
                     /locus_tag="PLC06_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90051.1"
                     /translation="MSTSFRASNRFNHNKPADGRLIRLMSHSVLGWLLIFSCLAFFAT
                     PASAQNNSRVQSYWQFAASGRLDTILNFDVDGDGVDEFLVLDENGHLTLLTADGRSEW
                     TYISQEPISALGTIKTNGGEESPTRQIALAGIGYLTLLDQHGEVVWRVSLNSSSAPVA
                     IDAFDYQSDGAEDILLLLNSGQLIAFNANGEVIWQFASQENSTA"
     gene            complement(644..1978)
                     /locus_tag="PLC06_06100"
     CDS             complement(644..1978)
                     /locus_tag="PLC06_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRO90052.1"
                     /translation="MGADNPFFHRGAIRHLEDFVGREAESAQMLGLLRNGQSVSLIGP
                     RRIGKSSLLLHLSNEAVREPYRLHSPHALFVMLDCQELAGSPPEEVYELLLDALLDAA
                     KQAGIKLATADQPGTYRALDRALQQVYQQGSSVVVILDEFELLAANQHLTPYFFARLR
                     GLTTKYGLAYLTASQRPLFAITAEEAVLSSPFFNIFVTMPLGLFTETEAHELFRRRLE
                     RTTVTFPPELAEYLIKLVGLHPFFLHIGGYHAVQGLINGEITPSSDGHLDEAALQRLD
                     NPIELEAESHLGYLWQNLSPEDKHVLAIADGPTESLRQLEQQCLLVKIDNVYTYSSEM
                     LRRFVRRQTVSDLIQAGPFVIDEQRHQVWGDGQELNLTTSQFSILVRLCQHVGQVVRG
                     EDLEMAVWGDVLVDDPDRLKTLIKRLRRAVDPFDNWIVSERGVGYALRSPEP"
     gene            2103..3401
                     /locus_tag="PLC06_06105"
     CDS             2103..3401
                     /locus_tag="PLC06_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRO90053.1"
                     /translation="MNDELGSLLRRLGVSKGARHLRSASPRQANEPSSRYEDATPPPL
                     EILLPGGRLLESAEGACFVVDRVFPLHYRHGADTLADLLALQPADGAPYISDERLNGQ
                     GFGDFLFLDTETTGLAGAGALAFMVGVAFFEPQPVLMDGIQTERQVLVVRQYFLRDHG
                     DEPAMLHQLDDLLIGKAGLITFNGRSFDVPLLDNRYLMNRRHGRLLDVPHIDLLPPAR
                     RLFRARLGSCALGALEQNLLGLQRTQDDVPGWLIPSLYHNFLHTGDARELTRVFYHNE
                     MDMLSMVTLAARMFRHMSTGACDDALDQISLGRWQMDLGLFDEAERTLRAALEDDATP
                     LEAYQQALGLLGGLYKRKERRPEAALVWQQLAAVNTADVSAHVELAKHFEWHASDMPA
                     AIEWTRRALALIDRLPPTPGTRLTRAELTHRLQRLEKKAS"
     gene            complement(3398..4615)
                     /gene="trpB"
                     /locus_tag="PLC06_06110"
     CDS             complement(3398..4615)
                     /gene="trpB"
                     /locus_tag="PLC06_06110"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRO90054.1"
                     /translation="MDTFTSVPDEKGKFGPFGGQFVPETLMPALEELIAAYEKARQDP
                     AFIAEFEHLLRSYVGRPSPVTYARNLSEKLGGAQIYFKREDLNHTGAHKINNALGQAL
                     LVKRMGKQRVVAETGAGQHGVATATVCALLGLQGVIYMGEVDIARQQPNVYRMHLLGA
                     EVRPVSSGSRTLKDAINQAIRDWVTNVSTTHYLLGSVLGPHPYPAMVRDFQSVIGREA
                     REQMLATTGRLPDAVVACVGGGSNAMGIFHAFLNDETVELIGVEAGGEGIPDGRHAAR
                     FADPAVARVGVLHGTKSYVMQTADGQIRDTHSISAGLDYPSVGPEHAWLRDQERAFYT
                     YATDEEALAAFHELSRTEGIIPALESAHAVAEAIKRAPTMGKDQILLVNLSGRGDKDL
                     DSVLRVMSERGVD"
     gene            4736..6100
                     /gene="trmFO"
                     /locus_tag="PLC06_06115"
     CDS             4736..6100
                     /gene="trmFO"
                     /locus_tag="PLC06_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRO90055.1"
                     /translation="MSKELIVVGGGLAGSEAAWQAAEAGIDVRLYEMRPGRMTPAHET
                     SWLAELVCSNSLGSNLPHKAPGLLKAELRGLGSLILDCATQTAVPAGSSLAVDRERFA
                     QLVTERVSGHPRITLVAEEVPAIPDAPVIIASGPLTSSSLAEDIAGLTGYEYLYFYDA
                     VSPIVQAETIDMNIAFRQSRYDKGEQEEGDYINCPMTRDEYEQFVGALRTAETITLKQ
                     FEREDPHFFEGCMPIEALAARGHEALAFGPMRPVGLIDPRTNRRPHAVVQLRQDNLIG
                     SLYNIVGFQTNLKWGDQKRVLRLIPGLENADFARYGMMHRNTYINSPVMLQPTMQYRN
                     RVDLFFAGQIVGVEGYMGNAGTGLLAGVNAARYLTGRHPVILPPTTMLGALCHYVTHS
                     AMKDFQPMKANFGILPPLPPRPGKEERGRHYAERALTAMRRFAREHGLRYDPAMAVAE
                     IQNA"
     gene            6155..7762
                     /locus_tag="PLC06_06120"
     CDS             6155..7762
                     /locus_tag="PLC06_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRO90056.1"
                     /translation="MKIPVETTVTSVVVYPDRARVTCVGRADLTEGLHQITVGELPLA
                     MEPESVRAAGSGTARVRLRSVDVQRRHYAAAPAPNVTALEARINQLQLELQALADRQA
                     VKQASIEHLDGLREATREYARGLARGRSTIEDQAGFMRFFEDEYARLHDALREMGVEQ
                     RALQDELTKLQAELNQIRSARPRQRYEAQLEIEALSAGDFAVEVSYIVGRAGWRPLYD
                     MRLQDAAASGAVVAMSSLAEITQDTGQDWTGVQLSVSTARPALNQRVPELKPWFVDVL
                     QPSPRMLRSRPMAKGVQMDALEMAADDEPVAMMAAMASPPVAAAEVEVATVQENGTAI
                     TFRVAGDVNIPGDGTPKKTALGQYELVPELDFLSVPRHTDAVYRRAKLINSTGAPMLA
                     GPVSLYVGDEFIGTNRLEYTPNGAEMELVLGVEERITVKRELVRREVDKRLLRDQRQI
                     LYGYEIKLENLLASRAIVTVQDQYPVSRHDQIKVRLDRATPTPVEQSELNILKWRLDL
                     EPGAKKTIQYDYQVEHPRALPVAGLLD"
     gene            7769..9085
                     /gene="hflX"
                     /locus_tag="PLC06_06125"
     CDS             7769..9085
                     /gene="hflX"
                     /locus_tag="PLC06_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRO90057.1"
                     /translation="MGTSIKGYDTAPPRERAFLVGVELRESPSLLPVEDSLDELARLA
                     DTAGIDVVGQAIQRIDKPNSATYIGPGKVEEVKLLVEELQADMVIFDDELSPRHQREL
                     EEIFGEEIKVIDRTALILDIFGQHAHTREGKLQVELAQLEYRLPRLTRMWTHLARQAG
                     GRAGGATGGVGLRGPGETQLETDRREIGRRISQIKEELENVRAHRERHRAKRQQTELQ
                     VVAIVGYTNAGKSTLLNKISGADVLTADMLFATLDPTTRKVNMPGGRELLFTDTVGFI
                     QKLPTQIVAAFRATLEEIGDADMLLHVVDVTHPNAAEQIEAVEDTLAELEADHLPMVI
                     ALNKADQLTNAEEVIGELDLSAPAALVSAKTGKGIEELLIMVEAAMVQLLQPVEVLIP
                     YTRGDLLSLFYERGQVDQEKHTGEGVHLFGRVPKRLIPVFEPFQPA"
     gene            9082..10254
                     /locus_tag="PLC06_06130"
     CDS             9082..10254
                     /locus_tag="PLC06_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO90058.1"
                     /translation="MKSINELSRNPDIRSALAEFQARLDGIVEQVIAIQQIPAPTFEE
                     ETRARFIEEQFKALGLEDVNRDALNNVYGRLPGTSSALPPVVLSAHLDTVFPMNTDLA
                     IRREGNLIYGPGIGDNSTGVAGLLQTAQAIRDNRLAHTADIYFVANVGEEGLGDLRGM
                     RAVVDRFGGDAIYIVVEGGLYGKLIHQAVGVRRYRIQISAPGGHSWGGFGAASAIHQL
                     GHLIVDIDSLAVPAIPKTTYNVGIIQGGISINTIANSAELWLDLRSEGVVALEQLVKD
                     VEEIVRTRNKRYALEGDGVEIKMHRVGDRPAGSISRQSPLVAGAEAALRAVGCEDVHY
                     IVSSTDANIPLSRGYEAVCLGLTHSGNSHRPDEFMDKTNLPAGLGQLLLVTLAAAG"
     gene            10356..>11437
                     /locus_tag="PLC06_06135"
     CDS             10356..>11437
                     /locus_tag="PLC06_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO90059.1"
                     /translation="MDFQLNEEQRALKEVVHDFVAREVRSRARHTDEASEFNWAATRK
                     MGPLGLLGLEVPEEYGGAALDAISVAIVIEELGWGCGSTALAIAAHNGLGLGPLVAYG
                     TEEQKRHWLPRLTSGEGKLACLSLTEPGAGSDLKDGVRTRAVADGDSWVINGAKMWMT
                     NASISDLMVVLCRTGDKGSHALSHILVPSDTPGVIIGPAEKKMGLHGSPTHAVTFDGA
                     RVPRENLLGVEGRGLPQTLATLDSGRVGIGALALGLAQAAYEEALKYAKEREAFGQPI
                     ANFQAIQWMLADAATEIEAARLMVYKAAWLKGLKQPYSTMASMAKLFATEVSERVCRN
                     AIQIHGGYGYSSEFEVERMYRDTRLMT"
BASE COUNT         2316 a         3260 c         3481 g         2380 t
ORIGIN      
        1 atggctgtgg agttttcctg actcgcaaac tgccaaataa cctccccatt ggcgttaaaa
       61 gcgatcaatt gaccggaatt tagcaggagg agaatatctt ccgccccatc tgactgataa
      121 tcaaatgcgt caatcgcaac cggcgcggat gaagaattga gcgacactcg ccatacgacc
      181 tcgccgtgtt gatccagcag ggtcaaatag ccgattccgg ccagcgcgat ttgccgggtg
      241 ggtgactcct ctccaccgtt tgtcttgatt gtccccaaag ccgagattgg ttcctggctg
      301 atatacgtcc attccgaacg gccgtcggcg gtcagtaagg tgagatgtcc gttttcatcc
      361 agcaccaaaa actcgtctac gccatcgcca tccacatcga agttgaggat cgtgtcgagt
      421 cggcccgaag ccgcgaattg ccaatagctt tggacgcggg agttgttctg ggctgatgca
      481 ggtgtggcga aaaatgccag gcatgagaat ataagcagcc agcccaggac tgagtgactc
      541 atcaaacgga taagacgtcc gtcggccggc ttgttatgat tgaaccgatt cgatgcgcga
      601 aacgatgtgc tcaattcgaa tgttgtcggc cctaataggc cgcttatggc tccggcgaac
      661 gtagcgcata gcctacgccg cgctcgctaa caatccagtt gtcgaaaggg tcgaccgccc
      721 gacgcaaccg tttgatcaac gtcttgagtc gatccgggtc gtcgaccaat acatcacccc
      781 aaactgccat ctcgagatct tccccgcgaa caacctgacc cacatgttgg catagccgca
      841 ccaggatgct gaactgtgat gtggtcaggt ttaactcctg tccgtcaccc cacacctgat
      901 gacgttgctc gtcgatgacg aacgggccgg cctggatgag atccgatacg gtttgccggc
      961 gaacgaagcg gcgaagcatc tcgctgctat atgtgtagac attatctatc ttcaccagca
     1021 aacactgctg ctcaagttgg cgcagacttt ccgtcggacc atcggcgatg gccaggacgt
     1081 gtttgtcttc cggtgaaagg ttttgccaga gatagcccag atgggattcg gcttccagct
     1141 caatggggtt atcgaggcgc tgaagcgcgg cttcgtcgag gtgcccatcg gaggatggag
     1201 ttatctcgcc gttgataagc ccctgtacgg catgatagcc gccgatatgc aagaaaaaag
     1261 gatgcaggcc gaccagcttg atcagatatt ccgctaattc cggcgggaag gtgacagtcg
     1321 tccgttccag ccgccggcga aagagttcgt gtgcctcggt ttcggtgaat aaccccaagg
     1381 gcatggtgac gaagatattg aagaatggcg agctgaggac agcttcttcg gccgtaatcg
     1441 cgaacaaggg acgctggctg gcagtcagat aggccaagcc atatttcgtg gttaaccccc
     1501 gcaagcgtgc gaaaaaatag ggtgtaagat gctggttggc cgccaataat tcaaactcat
     1561 ccagaatgac gaccacactt gatccttgct gatacacttg ctgcagggct cggtcgaggg
     1621 cgcgataggt tcccggttgg tcggccgtcg ccagcttaat gccggcctgt tttgccgcgt
     1681 ccagtagcgc gtccagcagc agctcataca cttcctcggg gggagagccg gccaactcct
     1741 ggcaatcgag catgacgaat agggcatggg gtgagtggag ccgataaggc tcgcgaacgg
     1801 cttcattcga cagatgaagg agaagtgagc tttttccgat gcgtctcggc ccaatgagcg
     1861 atacgctttg cccgttacgc agcaacccaa gcatctgggc ggattctgct tcacgaccga
     1921 caaaatcttc aagatggcga atagcgcctc gatggaaaaa aggattatct gcgcccatgt
     1981 tgatgcctat tgtaatgccg aaccgggcga aatggaaatc gttgtcacct gcgctcagcg
     2041 agttgtcacc gattgtatat tgtcccttgc tctgatgggg ccggaatcgt aaaatagaac
     2101 atgtgaacga cgaattgggt tcgcttctgc gtcgactcgg cgtgagcaaa ggggctcgtc
     2161 atttgcgttc ggcaagcccg cgccaggcta atgagccatc gagtcgctat gaagacgcga
     2221 cgccacctcc tttagagata ctgctgcccg gcggccgatt gctggaatcg gccgaaggcg
     2281 cctgttttgt tgtcgatcgc gtattcccgc tccactatcg ccacggcgcg gataccctgg
     2341 ctgacttgct ggcccttcaa ccggccgacg gcgcgcccta tatctcggac gagagactaa
     2401 acgggcaggg gtttggggac tttctctttc tcgatactga gacaaccgga ctggccgggg
     2461 cgggggcatt ggccttcatg gtcggtgtgg ccttctttga gccgcaaccg gtattgatgg
     2521 acggcatcca aaccgagcga caggttcttg tggtacgcca atatttcctg cgtgatcatg
     2581 gcgatgaacc ggcgatgctg catcagctgg atgaccttct catcgggaaa gcaggactga
     2641 tcacatttaa tgggcgttcg ttcgacgtac cgttgctcga taatcgctat ttgatgaacc
     2701 gccggcacgg ccgcttgctc gatgtccccc acattgactt gcttcccccg gcacgacgtc
     2761 tgttccgcgc ccggctcggt tcatgtgctt tgggcgcgct ggagcaaaac ctgctgggct
     2821 tgcaacgcac tcaggatgat gtgcccggct ggttgatccc ttcgctctac cataactttt
     2881 tgcacaccgg cgacgcgcgg gaattgactc gcgtctttta ccacaacgag atggacatgc
     2941 tctcgatggt caccctggct gcccggatgt ttcggcacat gtcgacgggt gcctgtgatg
     3001 acgcgctcga ccagattagc ctgggacgtt ggcagatgga cctgggatta tttgatgagg
     3061 cggagcgaac gctacgcgct gcgcttgaag atgatgccac cccactggaa gcatatcagc
     3121 aagccttggg tcttctgggc gggttataca agcggaagga gcgccgccct gaagccgctc
     3181 tggtctggca gcaattggcg gcagtcaaca ctgccgatgt ttcggcccac gtcgagctgg
     3241 ccaagcattt cgagtggcat gcgtccgata tgccggcggc tattgaatgg acgcgccgcg
     3301 ctctggcgct catcgatcgt ttgccgccaa cacccggcac gcgcctcacg cgggcagagt
     3361 tgacccaccg gctgcagcgt ctggagaaga aggcgagcta atccactccc cgctcgctca
     3421 tgacccgtaa gactgaatca aggtccttgt cgccgcggcc gctcaggttc accagcaaaa
     3481 tctggtcctt gcccatcgtc ggcgcgcgct taatcgcttc ggccacggca tgggcggact
     3541 ccagcgcggg gatgatgcct tccgtccggg atagttcatg gaaggcggcc agcgcctctt
     3601 cgtcagtggc ataagtgtag aaggcccgtt cctgatcgcg cagccaggcg tgttccgggc
     3661 cgacgctggg ataatccagc ccggcgctga tgctatgcgt gtcgcgaatc tggccgtcgg
     3721 ccgtttgcat cacatagctt ttcgtgccgt gaagcacgcc gacgcgcgcg acggccgggt
     3781 cggcgaatcg cgcggcgtgt cggccgtcag gtatgccctc gccccccgcc tccacgccga
     3841 tcaattccac cgtttcgtca ttcagaaagg cgtggaaaat gcccatcgca ttgctgccgc
     3901 cgccgacgca agccacgaca gcatcgggta accggccggt agtcgccaac atctgctcgc
     3961 gcgcttcgcg gccgatcacg ctctggaaat cacgcaccat cgccgggtag ggatggggcc
     4021 ccagaacgga gccaagcagg tagtgggttg tggacacgtt tgtcacccag tcgcgaatgg
     4081 cctggttgat ggcatccttc agcgtccggc tgcctgaact caccggtcgc acctcggctc
     4141 ccagcaagtg catccggtag acattcggct gctgccgggc gatatcgacc tcgcccatat
     4201 agatgactcc ttgcagcccc aacaaggcac agacggtggc tgtggccaca ccatgttggc
     4261 ccgcgcctgt ttcggctacg acgcgctgtt tgcccatgcg cttgaccagc aacgcttgcc
     4321 ccagggcgtt attgatcttg tgcgcgccgg tgtggttgag gtcctcacgc ttgaagtaaa
     4381 tttgcgcgcc gcccaacttt tccgaaagat tgcgggcgta tgtcaccggc gacggccgcc
     4441 cgacgtaact gcgcagcaaa tgctcgaatt cagcgatgaa tgccgggtcc tgccgtgctt
     4501 tctcataagc ggcgatcaac tcttccaggg ctggcatgag ggtttcgggg acaaactggc
     4561 cgccaaacgg cccaaatttc cctttttcgt ccggtactga agtgaatgtg tccatgctgt
     4621 tcattgtttc gggtcacgaa ggattggcgg attatagcaa taatcgctct atcggcggga
     4681 atagtcaggc atgacctgaa agctattaat tcacttgctg tgttagaata accctatgtc
     4741 aaaagaactt atcgttgtgg gaggcggcct ggcgggcagt gaggccgcgt ggcaggctgc
     4801 cgaagccggg atcgacgtgc ggctctatga gatgcggccg ggccggatga cgcccgcgca
     4861 cgagacctct tggttggcgg aactggtctg cagcaactcg ctcggctcca atctgccgca
     4921 caaggccccc ggattgttga aggcggaatt gcgcggcctg ggttcgctca ttctcgattg
     4981 tgcgacacag acggccgtac ccgcggggtc gtcgctggcc gtggatcggg agcgtttcgc
     5041 gcagctggtc accgagcggg tttccggcca cccgcgaatt actctggtgg cggaggaggt
     5101 tccggcaatt cccgacgcgc cggtcatcat tgccagcggc ccgctgacat cctcgtctct
     5161 ggccgaagac atcgccggct tgactgggta tgagtatctc tatttctacg atgccgtctc
     5221 gccgatcgta caggcggaga cgatcgacat gaatattgct ttccggcagt cgcgctatga
     5281 caagggggag caggaagaag gggactatat caactgcccg atgacccgtg acgagtatga
     5341 gcaatttgtc ggcgcgctca ggactgccga gaccatcact ctcaagcagt ttgagcgcga
     5401 agatccccac tttttcgagg gctgcatgcc catcgaggcg ttggctgcac gcggccatga
     5461 ggcgttggcc ttcggtccga tgcggccggt tgggttgatc gatccccgca ccaatcgacg
     5521 gccgcatgcc gtcgttcaat tgcgccagga caatcttatc ggctcgctct acaacattgt
     5581 cggtttccag accaatctga agtggggcga ccagaaacgg gtattgcgcc tcatccccgg
     5641 gctggagaac gccgattttg cccgctacgg catgatgcat cgcaatacct atatcaactc
     5701 tccggtcatg ctccagccca ccatgcagta tcgcaaccgg gtcgacctgt tttttgcggg
     5761 gcaaatagta ggggtcgagg gttatatggg taatgccggg acggggctat tggccggcgt
     5821 caatgccgcc cgttatctga ccggccgcca tcccgtcatt ctgccgccga cgaccatgct
     5881 gggagcgcta tgtcattatg tgacccacag cgcgatgaag gattttcaac cgatgaaggc
     5941 caacttcggc attctgcccc cgctgccgcc gcggccgggc aaggaggagc gcggccgcca
     6001 ctacgctgaa cgcgccctga cggctatgcg tcgctttgcg cgagagcacg gcctgcgcta
     6061 cgatcctgct atggctgtcg cggagatcca aaatgcgtga tcgtcgccag aggaaagccc
     6121 ccatcccgca accgacacgt gaggagacga acaaatgaaa attcccgttg agaccaccgt
     6181 cacatcggtc gttgtttacc ctgatcgagc acgagtcacg tgcgtgggtc gcgccgattt
     6241 gactgaggga ctgcatcaaa tcacggttgg agagttgccg cttgcgatgg agcctgaatc
     6301 cgtgcgtgcc gccggctccg gcacggctcg tgtccggctg cgcagcgttg acgtacagcg
     6361 gcgacattac gcggccgccc cggcccccaa tgtaacggcg ttggaagcgc gcatcaatca
     6421 acttcagttg gagctgcagg cgcttgccga ccggcaggcg gttaagcagg cgtcgatcga
     6481 acacctcgac ggattgcgcg aggcgaccag ggagtatgcc cgtgggcttg ctcgcggccg
     6541 cagcactatt gaggatcagg ccggctttat gcgcttcttc gaggatgagt atgctcgctt
     6601 gcatgatgct ctgcgagaga tgggtgtaga gcagcgcgcc ctccaggacg aactgacaaa
     6661 gttgcaggca gaactgaacc agattcgctc cgcgcggccg cgtcagcggt atgaagcaca
     6721 gctggagatc gaagccttgt cggccgggga ttttgcggtt gaggtgagct atatcgtcgg
     6781 ccgcgcggga tggcggccat tgtatgacat gcgattgcag gacgccgccg catccggcgc
     6841 ggtcgtcgcc atgagcagcc tggcggaaat cactcaagac accggtcagg actggacagg
     6901 cgttcaactt tccgtatcga cggcacgccc ggcgctcaat cagcgcgtgc cggaactcaa
     6961 gccgtggttt gttgatgttc tgcagccatc accaaggatg ctaaggtccc gcccaatggc
     7021 caaaggcgtt caaatggacg cgcttgagat ggcggccgac gatgaaccgg tggcgatgat
     7081 ggcagcgatg gcctcaccgc ccgtggcggc ggctgaggtc gaagtcgcca cggtgcagga
     7141 gaatgggact gcaatcacgt ttcgcgttgc cggtgatgtc aatattcccg gcgatggcac
     7201 ccccaagaag acagccctgg ggcagtatga actggttcct gaattggact tcctgtccgt
     7261 gccgcgccac actgatgccg tttatcggcg cgccaaatta ataaactcta ccggtgcccc
     7321 catgttggcg gggccggtca gtctctatgt tggcgacgag ttcatcggca caaatcgtct
     7381 ggagtacacg ccgaatgggg cggagatgga gctggtgctt ggcgtagagg aacgcatcac
     7441 cgtcaaacgg gaactcgttc gccgtgaagt agacaagcgt ctgttgcgcg atcagcgtca
     7501 gatcctttac ggttacgaga tcaagctgga gaatttgctt gcctccaggg cgatcgtcac
     7561 ggtgcaggac cagtatcctg tgtcgcgcca cgatcagatc aaggtgcgtc ttgatcgcgc
     7621 caccccgacg cctgttgagc agagtgagct taatattctg aagtggcgac tcgatttgga
     7681 accgggggcg aagaaaacaa tccagtacga ttatcaggtc gaacacccac gggccttgcc
     7741 cgttgccggg ctgctcgact aggaggtcat gggtacgtct atcaagggat atgataccgc
     7801 gccaccgcgc gaacgagcat ttctggtcgg cgttgagcta cgcgagagcc cctcgctttt
     7861 gccggtcgaa gacagcctcg atgaactcgc gcgattggcg gatacggctg gaattgacgt
     7921 cgtcggtcag gcaattcagc gcatcgacaa gccaaatagc gccacctata ttggccccgg
     7981 caaggtcgag gaagtgaagt tgttggtcga agaactgcag gctgacatgg taatttttga
     8041 cgacgaactc tcgccgcgac accagcgtga actggaagag atcttcggcg aagagatcaa
     8101 ggtcatcgat cgcacggccc tgattcttga cattttcggt cagcacgccc acacacgaga
     8161 gggcaagctg caagtcgagc tggcgcaact ggagtaccgc ttgccgcgcc tgacgcgcat
     8221 gtggacacac ctggcccgac aggccggtgg tcgcgccggc ggtgcgacgg gcggcgtcgg
     8281 tttgcgcggc ccgggcgaaa cgcagttgga gaccgaccgc cgggaaatcg gccgccgcat
     8341 ctcccagatt aaagaggaac tggaaaacgt tcgtgcccat cgtgaacgcc atcgcgccaa
     8401 gaggcagcag accgagttgc aggttgtggc cattgttggc tacaccaatg ccggtaaatc
     8461 gacgctcctg aacaagatta gcggcgcgga tgttctgacg gccgacatgc tttttgccac
     8521 gctcgacccc acgacacgga aagtcaacat gcccggcggc cgcgagcttc tgttcaccga
     8581 tacggtggga ttcatccaga aattaccgac gcaaatcgtg gccgcttttc gcgccacgct
     8641 ggaagagatc ggtgatgccg acatgttgct tcatgtcgtg gatgtgaccc atccgaacgc
     8701 ggccgagcaa attgaagccg ttgaggatac cctggccgaa ctcgaggccg accatctgcc
     8761 gatggtgatc gccctcaaca aggccgacca gctgacaaat gccgaagaag tcataggtga
     8821 actggatctg tcggctcccg cagcgctggt ctcggccaaa acgggcaagg gcatcgagga
     8881 gctgctcatc atggtcgagg cggcgatggt ccagttgctc cagcctgtcg aggtgcttat
     8941 cccctacacc cgcggcgatt tgttatcact gttctatgag cgaggacagg ttgatcagga
     9001 gaagcacacc ggtgaaggcg tgcacctgtt cggccgtgtt cccaagagat taattcccgt
     9061 gtttgaacct tttcagccgg catgaaatca ataaacgaac tttcccgtaa tcccgatatt
     9121 cgctccgctc tggcggaatt ccaggctcgc ctcgatggta tcgtcgagca ggtgatcgcc
     9181 atccagcaga tccccgcgcc gactttcgag gaagagacgc gcgctcgctt cattgaagaa
     9241 cagttcaagg ctctcgggct tgaagatgtc aatcgtgatg ccttgaacaa tgtctatggt
     9301 agattgcccg gcacatcgtc cgcgctcccg ccggtcgtcc tttccgccca tctggacaca
     9361 gtcttcccga tgaacaccga tttggctatt cggcgggaag gcaacctgat atacggaccg
     9421 ggcatcggcg ataattccac cggcgtggcc ggattgttgc aaacggcgca ggccattcgg
     9481 gacaaccggc tcgcgcacac ggccgatatc tattttgtcg ccaatgtggg cgaggaaggg
     9541 ttgggcgatc tgcgcggaat gcgcgccgta gtcgatcgat tcggcggaga cgcgatctat
     9601 atcgtcgtcg agggcggcct gtacggcaag ctcatccacc aggctgtggg tgtcaggcgc
     9661 tatcgtattc agatctccgc gcccggcggt cactcctggg gcggtttcgg cgcggccagc
     9721 gccatacacc agttgggtca tctcatcgtc gatatcgaca gcctcgccgt gcctgccatc
     9781 ccgaaaacga cctacaatgt cggtattatt cagggcggca tctcgatcaa caccattgcc
     9841 aattcggccg aactgtggct cgatctgcga tcggaaggcg ttgtcgcgct ggaacaactc
     9901 gtaaaggatg ttgaagagat cgttcgcacg cgcaacaaga ggtatgccct tgagggtgac
     9961 ggagtcgaaa taaaaatgca ccgggtgggt gatcgcccgg ccggcagcat tagccgccag
    10021 tcccctctgg tggcaggcgc ggaggccgct ctgcgagcgg tcggctgcga ggacgtgcat
    10081 tatattgtca gcagtaccga cgccaacatc cccttgagcc gtggctatga ggctgtgtgt
    10141 ttgggcttaa cccactccgg gaactcccat cgaccggatg agtttatgga caagacaaat
    10201 ctgccggcgg ggttgggtca gttgttgttg gtgactctgg ccgcggcggg atagccgccg
    10261 taatggccgt gatggtggag tcgtcagaag gctttgcggt ccggccttct tccggttttt
    10321 gattatgtta aatagcattt acaggtgaca ttcccatgga ttttcaactg aacgaagaac
    10381 aacgcgcatt aaaggaagtc gtccatgact tcgtggcacg cgaggtgcgc tcacgggcgc
    10441 gacatacgga tgaggcgagc gagttcaatt gggcggccac gcgcaagatg gggccgctgg
    10501 gcttgttggg gcttgaggtt cctgaggagt acggcggtgc ggcgctcgat gcgattagcg
    10561 tcgctatcgt aatcgaggag cttggctggg gttgtggttc cacggcattg gctatcgctg
    10621 cccataatgg cctgggtctc ggccctctcg tggcttatgg tactgaagaa caaaagcgac
    10681 actggctgcc tcgattgacc agcggcgaag ggaagctggc ctgtctgagc ctgacggagc
    10741 cgggcgccgg ttcggaccta aaggacggcg tgcggacgcg ggctgtggct gatggcgatt
    10801 cctgggtcat caacggggcg aagatgtgga tgaccaacgc ttctatttcc gatctgatgg
    10861 tcgtcctttg ccgcacggga gataaaggca gccatgcact gagtcatatt ctagtgccgt
    10921 ccgatacccc cggcgtcatc atcggcccgg ccgagaagaa gatggggctt catggttcgc
    10981 ccactcatgc cgtgaccttc gacggcgcgc gcgtgccgcg cgagaatttg ctgggcgttg
    11041 aagggcgcgg gttgccgcag acgctggcga cgctggatag cggccgcgtc ggcatcgggg
    11101 cgctggcact gggtctggcc caggcagcct atgaggaagc gttgaaatac gctaaagagc
    11161 gcgaggcttt cggccagcca atcgccaact tccaggccat ccaatggatg ctggccgacg
    11221 cggcaacgga gatcgaggct gcgcgactca tggtctacaa agccgcctgg ctgaagggtc
    11281 tgaagcaacc ctattcaacc atggcctcta tggccaaact cttcgcgacc gaagtcagtg
    11341 agcgcgtgtg ccgtaacgct atccagattc atggtggcta cggctacagt tccgagttcg
    11401 aggtggagcg catgtatcgc gacacgcgac tgatgac
//
LOCUS       DAOMQG010000075        11402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2454_length_11402_cov_8.391469, whole genome shotgun sequence.
ACCESSION   DAOMQG010000075 DAOMQG010000000
VERSION     DAOMQG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2454_length_11402_cov_8.391469"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..926
                     /locus_tag="PLC06_06140"
     CDS             63..926
                     /locus_tag="PLC06_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HRO90060.1"
                     /translation="MDNDDEPIGRLLTRREMLKLLAALGAATAAGCAPRLSGIATAPT
                     ATTGATTGATTVPTATGIAGVGGAIPTATAAPDPTAAEALAATVPVPACVVRPEQTEG
                     PYFVDVRLDRSDIRADADGTVKPGAPLALAFLVSRVGETSCTLLPGAMVDIWHCDADG
                     VYSGVNDRNFGSTAGQTFLRGYQVTDANGTAAFTTIYPGWYSGRAVHIHFKIRAELDG
                     RHYEFTSQLYFDDALSDRVLAQEPYSARGDRNTRNDADGIFGRGGDLLTLNVTESGGG
                     YAAVFDIGMQV"
     gene            complement(1012..1827)
                     /locus_tag="PLC06_06145"
     CDS             complement(1012..1827)
                     /locus_tag="PLC06_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016202210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRO90061.1"
                     /translation="MDLFDVINNHRSIRRYTTDPVPDELLNRALEAGLRASSSGNMQT
                     WSVIVTRDRALREALYAPHMEQEMVLDAPVLLTFCADFRRMRHWLRLNDAPDNFDNFM
                     SFMIATIDATLASQNVALAAEALGLGICYMGSTLANCDQIGRVLGLPPGVFPVVGYSL
                     GWPAEDPKPRDRLPLSALVHHETYQDYSDERITAIYAERNRKGWERYMTYPELRARIE
                     GSGVENLAQIYTVLKYTRESHLDFSRTVLDYLVAQGFMENGERESADFADLGS"
     gene            complement(1952..2521)
                     /locus_tag="PLC06_06150"
     CDS             complement(1952..2521)
                     /locus_tag="PLC06_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HRO90062.1"
                     /translation="MRIGGIYSFNQGHEVISSRYPGELEEVRNVIASIDAQLYKTKTG
                     REKTRAGRTLFHPGSLNNAYKAAFSVMDWKTHRVSCEYPANFYTTDYTPKSGVRGAFR
                     EMDFVKNRVGVEVQFGKYAFMVYNVCAKMTIFHNEDVIDVGIEIVPLKELALEMSSGV
                     SYFEQIVWDLEHRGVSNIDIPVLILGVGP"
     gene            complement(2518..3507)
                     /locus_tag="PLC06_06155"
     CDS             complement(2518..3507)
                     /locus_tag="PLC06_06155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRO90063.1"
                     /translation="MSNLPLFQEPKTIEPAYSADSELVLYHGDTSEFIDTIPDESVKL
                     IVTSPPYNLGKAYEDKVSIEYYLELQAQTIEKFYRILHPQGSICWQVGNFVEKGEVYP
                     LDIYYHPIFKRMGYQLRNRIIWHFEHGLHTSKRFSGRYETLLWFTKTDDYTFNLDPVR
                     VPAKYPGKRHYKGPNKGKPSGNPQGKNPSDVWEIVAQDWEDCFWDIPNVKSNHPEKTI
                     HPCQFPVELVERCVLALTQEDDWVFDPYAGVGTTLIAATRHGRRGMGSEQDEAYVSTA
                     RDRLASYFTGQLKIRPLGKPIHKPSGNEKVSQVPPEWLGAEQKRLMEKGNKYE"
     gene            complement(3597..5633)
                     /locus_tag="PLC06_06160"
     CDS             complement(3597..5633)
                     /locus_tag="PLC06_06160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90064.1"
                     /translation="MKDKTAAAWFIVILLLIIAGQWIIGRAVIESPGGVSPWPAVFLL
                     IAAAMGLQLATESRPDDPPQAEAAADPDEPGPDPGHPGPYVFHAVPPLPADRRGTIFI
                     LIFLLLGFVLWRIPAMARDANYWPVFLAWLAAVLLFLAAVRQPGEGGVSRPWLRNRRG
                     LLLGIGALAALALLLRVWRIGDIPFTLSGDEASQGLDALKFSAGEWRNPFTTGWLGVP
                     TMSFIYNSISLAWFGPTVTGLRLPWALVGAATVITTFLLVRQLFHTGLATLTAFLVAV
                     YHYHIHFSRLGSNQIADPLFMSLALLFLYRGLDRGRRLDWALSGVITGLALYFYAGAR
                     LTPLVMIAVLGYLFLIGPRRFWADHRGGVAALALAAFITAAPMLQYAARFPDDFNARI
                     NQVGIIQNGWLENEVANGRPMTDALFDQFQRAALAFNSWPDRTVWYGLTEPLLDPLFG
                     AIFLMGMIYGLAQLLNPKVGARLAPMVAWWWGGMILGGMLTESPPSSQRLITLSVPAM
                     FFVAYVLWELVELARRARPGLPHRAALVVMAALFAFISLTTYFVDYTPRRLYGGANAE
                     LATTIAPQLNALKGDHSFFMVGPPFMYWGFATLPFLVPDATAQDIEQPLTDPRAEGFA
                     PSGRGLVYIVHPARSAELGLLRTAFPNGEERAVLSGGVDGRVLGTLYIVPPGIH"
     gene            5781..6083
                     /locus_tag="PLC06_06165"
     CDS             5781..6083
                     /locus_tag="PLC06_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation protein"
                     /protein_id="HRO90065.1"
                     /translation="MVVPDGEWEAVGRDAHAVIDEAKAAGVYVFGGIDEAVPPVLVSA
                     DGSVAEGGYPWTPPLDGGFTVLELPSREEAVAWAARIAKACRCEQELRVFMFDPRS"
     gene            complement(6434..7393)
                     /locus_tag="PLC06_06170"
     CDS             complement(6434..7393)
                     /locus_tag="PLC06_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90066.1"
                     /translation="MGDEMMLEGLARIRVEEAIRTGLKEQHTRRNLPPESRPNLMGHL
                     RILVLVLTITILSTGCSAIAGTTSSDELEPVTESGPSRFTITMTPEKIDIPADIPAGL
                     VALTIDNADSEWHSAIFRKLNQDVSLEDFEAAFRQDPRSTLPMTTFIGGPDVPGKTTM
                     PGYYPLEAGTYIVVDNWTEPWRFASFKVEGQTAQEMQPPRADVQVMMDEYTIEMPDSF
                     PKGRRLWQFTNQGEFPHNVAIIRLSEDQSLDDIAVWLNEEPGPEPFEYFTMWNIVSPG
                     ITSWGEIELQPGEYLAVDFMPDFGSEDGLNIDQGMVSAFTVTP"
     gene            7521..9851
                     /locus_tag="PLC06_06175"
     CDS             7521..9851
                     /locus_tag="PLC06_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRO90067.1"
                     /translation="MDNTSKPSWMEEFSHREIEILGLISDGLSNREISRRLYLSPDTV
                     KWYNKRIFSKLGVNSRTQAINLARRYSLTESQPEKHLQPIHNLPAQMTSYVGRKQEIA
                     DIQQLLTTARLVTLTGSGGTGKSRLALQVAGLSSARYRDGVWLVELASLSDPTLVPDA
                     IARGLKLHVSGDTPLIEVLKINLTHKQLLLLLDNFEHLLDAAPLVTELLTAAPQLTIV
                     ATSRERLRLYGEVEYPVRPLALPDRDRHNAAQELPGYEAVDLFIQRARAAHPGFEVDD
                     VNVFAIADICRRLDGLPLAIELAASRVKIYPPANLVQRLDHSLDALPVGPRDLPARQR
                     TLRATIEWSTDLLQTDEKLLLSRLSVFNNGGTLESIQGICAAGLSENSLDALTSLVDK
                     NLVFVREGRDGEPRFLMLETISEFAGELLAANEETHAIQRSHAAYFAELAERYSQEVR
                     SQRHVYWNAHFHQEQDNLNAALAWSLTGTEPLFGLRQIAGMMDHWWYNGARKDLPWAE
                     LALSKIEVAPPDLQAAVFRSVATLYLNIGDAAGSGKLHRRSVDLFQQLGDERNAAWSM
                     MGLSFSNSENPDDIEDCLAIAQQSVATLRRFGDKPGIARALTAVGELWRIKGDYEAAK
                     SCYTESLALSQETGERIRQAVQFGNLGFTAYHQGHYQAAIEYIQQGLVIVEEMEIIPE
                     VPTWLYSLAGPVAALGYPLHAAHLLGTADSILNTVGVELQPADRHDVLPIIESVRRTL
                     GEAEYQQAWQAGYELTLQQAIEFALGIELRGAKGKE"
     gene            complement(9997..10170)
                     /locus_tag="PLC06_06180"
     CDS             complement(9997..10170)
                     /locus_tag="PLC06_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90068.1"
                     /translation="MTLSSPKMITWVIALVLGVIGILANLVSLPIVSAGFGFWLLVIG
                     FVLLAAGSVVKGL"
     gene            complement(10407..11327)
                     /locus_tag="PLC06_06185"
     CDS             complement(10407..11327)
                     /locus_tag="PLC06_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90069.1"
                     /translation="MSTERMQVLQLLSMGKLTIDEAELLLAALAESESAAPAPDMAAL
                     KAAEQWAGPFEFGWAPVPPVPPVPPVPPRAPVPPRAPIPPRTGRGRRADVIDEAIRLR
                     NQGIDAAYLNKMRAALGDIAIDDVIALGIHGIEPEYVREMRDLGFDLTVDDVVRMGIH
                     GVDIEFVRRARKLGFDHLTTDDLVRMSIHGVDEEMLAMLEKAGLTDVTADELVQMGIH
                     GVDEELIDMMRQMDFTELSAGDLTAMSIHDVTPEFVAEMKAAGYADLSADDLVRLRAN
                     GVTGSYIEKLRRAGLDHLTVEELIAQRSAH"
BASE COUNT         2409 a         3350 c         3424 g         2219 t
ORIGIN      
        1 taacgcgcct ttacgcagca gcggctttgc gctacattcc ggcaactttc cggagacacc
       61 acatggacaa cgacgacgaa cccatcggcc gattattgac gcgacgagag atgctgaagc
      121 tgctggcggc gctgggcgcg gcgacggccg ccgggtgtgc cccgcgcctg tcgggaatcg
      181 cgaccgcgcc gacagccacg acaggggcca cgacaggggc cacgacagtg cccacggcga
      241 ccggaatagc cggcgtcggc ggagccatcc ccacagcgac ggccgccccc gacccaacag
      301 cggccgaggc gctcgccgca accgtccccg tgcccgcctg cgtcgtccgg ccggagcaaa
      361 ccgaagggcc ttacttcgtg gacgtgcggc tcgaccgcag cgacatccgc gccgacgccg
      421 acgggacggt gaagccgggc gcaccgctgg cattggcgtt cctcgtctcg cgcgtgggcg
      481 agacgagctg caccctgctg cccggcgcga tggtcgacat ctggcattgt gacgccgacg
      541 gcgtctactc cggcgtcaac gaccgcaact tcggcagcac cgccgggcaa acgttcctgc
      601 gcggctatca ggtgaccgac gccaacggca cggccgcctt cacgaccatc tatcccggct
      661 ggtattcggg gcgcgccgtc cacatccatt tcaagattcg cgccgaactg gacggccgcc
      721 actacgaatt cacctcgcaa ctctatttcg acgacgcgct atccgaccgg gttttggccc
      781 aggagccata cagcgccagg ggcgaccgca acacgcgcaa cgacgccgac ggcatcttcg
      841 gccgcggcgg cgacttgctg acgctcaatg tcacggagag cggcgggggc tatgcggccg
      901 tgtttgatat cgggatgcag gtgtgatggg ggctatccct aaaaaaccga gtttctcgga
      961 agaaacccgg tttttatgat cttaaggggt ttctcggaag aaacccggtt tttatgatcc
     1021 caaatctgcg aaatctgcgg attcccgttc cccattctcc atgaacccct gggccaccag
     1081 ataatccagc accgtgcgcg agaagtcgag gtgggactcg cgggtgtatt tgagcacggt
     1141 gtagatctgg gccaggttct ccacgccgga gccttcgatg cgcgcccgca gctcgggata
     1201 ggtcatgtag cgctcccagc ccttgcggtt gcgctcggca tagatggcgg tgatgcgctc
     1261 gtcgctgtag tcctgatagg tctcgtggtg gacaagggcg ctcagcggca gccggtcgcg
     1321 cggcttgggg tcctcggccg gccagccgag cgaatagccg acgacgggga agacgcccgg
     1381 cggcagcccc agcacgcggc cgatctggtc gcaattggcc agcgtgctgc ccatgtaaca
     1441 gatgcccagc cccagcgcct cggccgccag ggccacattc tgcgaggcca gcgtggcgtc
     1501 gatggtggcg atcatgaagc tcatgaagtt gtcgaagttg tcgggcgcgt cgttgaggcg
     1561 cagccaatgg cgcatgcggc gaaagtcggc gcagaaagtg agcagcaccg gcgcatcgag
     1621 caccatctcc tgctccatgt gcggcgcata gagcgcctcg cgcagcgcgc ggtcgcgggt
     1681 gacgatgacc gaccaggtct gcatattgcc cgacgacgaa gcacgcagcc cggcctccag
     1741 cgcccggttg agcaactcgt cgggcacggg atcggtggta tagcggcgga ttgatcgatg
     1801 attattgatg acgtcgaata agtccatggg ggtggattat cccacatctg cgggggttgg
     1861 gaagatattt atctaatgag aaactgcccc ctttgatttc cgctcacaag gtgtgattgg
     1921 gcggcaaaaa cggaggaaca agtggcccct gttatgggcc gacgcccaga atcaaaaccg
     1981 ggatatcaat gtttgagacc ccacgatgct caaggtccca cacgatttgc tcaaaatatg
     2041 acacgccact tgacatttcg agggccaact ctttcagggg gacgatttca atgccgacgt
     2101 cgatcacatc ctcgttatga aaaatagtca tcttggcaca aacgttataa accatgaagg
     2161 cgtacttacc gaattgaact tctactccca cccggttctt cacaaaatcc atttcgcgaa
     2221 acgcgccacg tacgcccgac tttggcgtgt aatcggtggt gtagaaattg gcgggatatt
     2281 cgcaagaaac cctgtgcgtt ttccaatcca tcactgaaaa agccgccttg taggcgttgt
     2341 ttagcgatcc tggatgaaac aaggtccgcc ccgccctggt tttttcgcgg ccggtttttg
     2401 ttttatagag ctgggcatca atcgaggcaa ttacgtttct cacctcttcc agttcgccgg
     2461 gataccggga agaaatgacc tcatggccct gattaaagga atatatcccg cctattctca
     2521 ttcgtactta tttccttttt ccattaatcg tttctgctcg gcgccaagcc attcgggtgg
     2581 aacctgggag accttttcgt tgcctgacgg cttgtgaatg ggcttgccca acggccgaat
     2641 ttttaattgg cccgtgaaat aggatgcgag cctgtcacgc gcggttgaca catacgcctc
     2701 atcctgttcg ctccccatgc ctcgcctgcc gtgccgggtg gcggcgatta acgtggttcc
     2761 gactccggca tatggatcaa aaacccagtc atcctcctgg gtaagcgcaa gaacacacct
     2821 ttccacaagc tccacgggga actgacatgg atggatcgtc ttttccggat ggttcgattt
     2881 aacgtttgga atgtcccaaa aacaatcttc ccagtcctgg gcgactatct cccacacgtc
     2941 cgacgggttt ttgccttgcg gattgccgga aggttttccc ttgtttggcc ctttataatg
     3001 ccgttttccg ggatacttgg ccgggacacg aaccgggtcc aggttgaacg tgtaatcatc
     3061 tgttttggta aaccagagga gggtttcgta acggccggag aaccgtttgc tggtatgcaa
     3121 tccgtgctca aaatgccaga taatccggtt tcgcagttgg tatcccatcc tcttaaaaat
     3181 ggggtggtaa tagatatcca gcggatatac ttcgcccttt tccacgaaat tacccacctg
     3241 ccagcaaata ctgccttgcg gatggagtat gcgatagaac ttctcgattg tctgagcttg
     3301 aagctccaaa taatattcga tggaaacctt gtcttcgtat gcctttccaa ggttataggg
     3361 gggagaggtg acgataagct tgaccgattc atccgggatg gtgtcgatga attcggatgt
     3421 gtcgccgtgg tataaaacca gttccgaatc ggccgaatag gcaggctcta tcgtttttgg
     3481 ctcctgaaat aacggcaaat tgctcatata cttcccatga gaacggttgt gctaaagcga
     3541 tggcaagatg ataacctctt ataggggtta cggcaattta tctcgatcag ggcacgtcaa
     3601 tgaatccccg gcggcacaat atacaacgtc cccaatacgc gcccatcgac cccgcccgac
     3661 agcacggcgc gctcctcgcc gttggggaaa gcggttcgca gcaggccgag ttcggccgag
     3721 cgggccgggt ggacgatgta gaccaggccg cggcctgatg gggcgaaccc ttcggcgcgg
     3781 gggtcggtca gcggctgctc gatgtcctgg gcggtggcgt cggggacgag aaagggcagc
     3841 gtggcaaagc cccaatacat gaacggcggc ccgaccatga aaaacgagtg gtcgcccttc
     3901 agcgcgttca gctggggggc gatggtcgtg gccagttcgg cgttggcccc gccatagagc
     3961 cgccgggggg tataatcgac gaagtaggtg gtcagactga tgaaggcgaa cagggcggcc
     4021 atgaccacca gcgccgcgcg atggggcagc cccggccgcg cgcgtcgagc caactcgaca
     4081 agctcccaca acacataggc cacgaagaac atcgccggga cgctcagggt gatgagacgc
     4141 tggctcgacg gcgggctttc ggtcagcatg ccgccgagga tcatgccgcc ccaccaccag
     4201 gcgaccatcg gcgcgaggcg cgcgcccacc ttcgggttca gcaattgggc cagtccgtag
     4261 atcatcccca tcaggaagat ggccccgaaa agcgggtcga gcagtggctc ggtcaggcca
     4321 taccacacgg tgcggtcggg ccaggagttg aaggcgaggg ccgcgcgctg gaactggtcg
     4381 aacagggcgt cggtcatggg gcggccgttg gccacttcgt tctccagcca gccgttctgg
     4441 atgatgccga cctggttgat gcgggcgttg aagtcgtcgg ggaagcgggc ggcgtattgc
     4501 agcatcggcg cggcggtgat gaacgcggcc agagccagcg ccgccacccc gccgcgatgg
     4561 tcggcccaaa agcggcgcgg cccgatgagg aacaggtagc ccagcacggc gatcatcacc
     4621 agcggcgtca gtcgcgcccc ggcatagaag tagagcgcca gcccggtgat gacgcccgac
     4681 aaggcccaat cgagccggcg gccgcgatcc agcccgcgat agagaaacag cagggccaat
     4741 gacatgaaca gcgggtcggc gatctggtta gagccgaggc gggaaaagtg gatgtggtaa
     4801 tggtagacgg ccacaaggaa ggcggtcagc gtggccaggc cggtgtggaa cagttggcgc
     4861 accagcagga aggtggtgat caccgtggcc gcgccgacga gcgcccacgg caggcgcagc
     4921 ccggtgacgg tcgggccaaa ccaggccagc gagatgctgt tataaatgaa gctcatcgtc
     4981 ggcacgccga gccagccggt ggtgaacggg ttgcgccatt cgccggccga gaatttgagc
     5041 gcgtccagcc cctgcgaggc ctcgtcgccg ctgagagtga acgggatgtc gccgatgcgc
     5101 cagacgcgca gcagcagggc cagcgccgcc agcgccccga tgcccagcag cagcccgcgc
     5161 cggttgcgca gccacgggcg cgagacgccc ccctcgcccg gttggcgcac ggcggccaga
     5221 aagagcagga cggccgccag ccaggccaga aacaccggcc agtagttggc gtcgcgggcc
     5281 atggcgggga tgcgccacag gacgaagccg aggagcagga agatcaggat gaaaatcgtg
     5341 ccgcgccggt cggccggcag cggcgggacg gcgtggaaga cataggggcc gggatggccg
     5401 ggatcggggc caggttcgtc ggggtcggcg gccgcttcgg cttgcggggg gtcgtccggt
     5461 cgggactcag tggccagctg caagcccatc gccgcggcga tcagcaggaa cacggccggc
     5521 catggcgaca cgccgccggg cgactcgatg acggcgcggc cgatgatcca ctggccggcg
     5581 atgatgagga gcaggatgac gatgaaccag gcggccgctg ttttgtcttt catggcatgg
     5641 atcgatgtcc ggattttacg gcgtaaggag aattatgatc gatgtggggg gagatgtcga
     5701 ggggggtgat catgcagagg taattgtagg ggcggggtta aaacccgccc cgacgagaga
     5761 tgctgaaact gctggcggcg atggtcgtgc ccgacggcga gtgggaggcg gtcggccgcg
     5821 acgcgcacgc cgtgatcgat gaggcgaagg ccgccggcgt gtacgtcttc ggcggtatcg
     5881 atgaagctgt gccgcccgtt ctcgtctcgg ccgacggctc ggtcgccgag ggcggctacc
     5941 cgtggacgcc tccgcttgac ggcggcttca ccgtgctcga actgccctcg cgcgaggagg
     6001 ccgtcgcgtg ggccgcacgc attgctaaag cctgccgctg cgagcaagag ctgcgcgtct
     6061 tcatgttcga cccacgctcc tgagggcggc attgaaatgg cgcaggaaat gtaacctgac
     6121 aattcattca aaccgaaccc gctttgaagg tcggttcaac tcaggcaccc gatgggcaaa
     6181 gcccgccggc aaatcaaacc cgtaggtcaa ctatggaaac atcatcgaat aaaatgcgct
     6241 cgctcgtgtg gatcagtctg ggcgcagcgg gaattgcgct tctgccggcg acccacgact
     6301 tcgctcacac aaacccgtcc aactaaagac tggagtgcat ataaccaaag cggccgtagt
     6361 atagacaatt cgatgtcagt accacggcca ctttgccctg ggcaaatgaa gtcgcgattc
     6421 cggccggact ttcttacggc gtcaccgtga aggccgagac catgccctgg tcgatattca
     6481 gcccatcctc actgccaaag tccggcataa agtctacagc caaatattcc cccggttgca
     6541 gttcgatctc accccaactg gttatgccgg gggaaacgat gttccacatg gtgaaatatt
     6601 caaatggctc aggccccggt tcctcattta gccagaccgc aatatcatcc aggctctgat
     6661 cttcactcaa ccgaatgatc gccacattgt ggggaaactc gccctggttg gtaaattgcc
     6721 acagccgcct gcctttggga aacgagtccg gcatttcaat ggtatattca tccatcatca
     6781 cctgaacatc ggccctgggt ggttgcatct cttgcgctgt ttggccttcg accttaaaac
     6841 ttgcgaaacg ccacggctca gtccagttat cgacaacaat ataggtgcct gcctccaggg
     6901 gataataacc gggcatggtt gttttacccg gcacatcagg gccaccaatg aaggtggtca
     6961 ttgggagagt gcttctgggg tcctgccgaa aggcagcctc aaaatcctcc agagaaacat
     7021 cctgattcag cttgcggaaa atggccgaat gccattcgct atcagcattg tctatggtca
     7081 gtgcaaccaa cccggccgga atatcggccg ggatatcgat cttttccggg gtcatcgtga
     7141 tagtgaaccg ggacggccct gactcagtga caggttccag ctcatccgat gaggttgtgc
     7201 cggctatcgc cgagcaaccg gtggacaaaa ttgtaattgt caacaccaac acaagaatac
     7261 gtaagtgccc catcaaattc ggcctgcttt ccggcggcaa gttacgccgg gtatgttgtt
     7321 ctttcaaccc tgtccggatc gcttcttcca cccgaattcg tgccagccct tctaacatca
     7381 tttcatcacc catcctcaga aatctggact gattaacatc ttcgagtgta aaggatatac
     7441 ggggggccgg tcaccaccct taagtgtgga aatggtatgg aattggctat actggatagg
     7501 cagaattccg ggtaaagaca atggacaata cttcaaagcc gtcctggatg gaggagttta
     7561 gccaccgcga aatagagatc ctcggcctga tttcggatgg cctgtcaaac cgtgaaattt
     7621 cccggcgact gtatctttcg cctgataccg tcaagtggta caacaaacgc attttttcaa
     7681 aactgggggt caatagtcgt acacaggcca taaatttggc tcgccggtac agtctgacgg
     7741 aatcacagcc ggaaaaacac cttcagccca tccataacct gcctgcacaa atgacctcct
     7801 atgtgggccg caagcaggaa atcgccgata ttcaacaact cctgacaacc gctcgtctcg
     7861 taaccctgac aggttccggg ggtacgggca aatcccgttt ggcgttacag gttgccgggc
     7921 tgtcatctgc ccgttatcgt gatggcgtgt ggctggttga gctggcttct ctgtccgacc
     7981 cgaccctcgt gccggatgcg attgcccggg gattaaagtt acacgtcagc ggcgacactc
     8041 cgctcattga agtgctcaaa attaacctga cacacaaaca gttgttactg ctattggata
     8101 actttgagca tctactggac gcggccccgt tggtgacgga actactgacc gctgccccgc
     8161 aactgaccat cgtggcgaca agtcgggaac gactgcgtct gtacggagag gtggagtatc
     8221 cggtcagacc actcgctctg ccggacaggg atcggcataa cgctgcccaa gagttgccgg
     8281 gttatgaggc tgttgatctg tttatccagc gcgctcgggc ggcccatccc ggttttgagg
     8341 ttgacgatgt gaatgttttc gccattgccg acatctgccg gcgtttggat ggtttgcccc
     8401 tggccattga gctggcggcc tcacgagtca agatatatcc ccccgcgaat ttggttcaac
     8461 gtctcgacca cagcctggat gcgcttcccg ttggcccccg cgacttgccg gcccgccaac
     8521 gcaccctgcg cgccaccatt gagtggagta ccgaccttct tcagaccgat gagaaacttt
     8581 tgctgtcccg tttgtcggtg tttaacaatg ggggcacttt ggaaagtatc cagggtatct
     8641 gtgcagcagg attgagcgag aacagtctgg atgctctcac ctcactggtc gacaaaaacc
     8701 tggtttttgt gcgggaaggg cgggatggcg aaccgcggtt tcttatgctg gaaaccatca
     8761 gcgagttcgc cggcgagttg cttgcagcca acgaggaaac gcacgcaatc caaagatcgc
     8821 atgcggccta ttttgccgag ctggccgaac gctacagtca ggaggttcgg agtcagcggc
     8881 acgtgtattg gaacgcgcac tttcatcaag aacaggacaa cctgaacgca gctctggcct
     8941 ggtcattaac cgggacggaa cctctgttcg ggctacgtca gatagcaggg atgatggatc
     9001 attggtggta caacggcgcg aggaaagatc tcccctgggc agaactcgcc ctaagcaaga
     9061 tagaggttgc cccacctgat ttgcaggcgg ccgtattcag gtcagtggcc acactttatc
     9121 ttaatattgg tgatgcagca ggatcgggga aattgcatcg tcggtctgtc gatctcttcc
     9181 aacaactcgg ggacgagcgc aacgccgcct ggtcaatgat gggcctgagc ttctccaaca
     9241 gtgagaaccc cgatgatatt gaggactgcc tggccatcgc gcagcagagc gtggcaacac
     9301 tgcgccgatt cggagacaaa ccgggtatcg ccagggcgct aacggctgtg ggcgaactat
     9361 ggcgcataaa aggcgattac gaggcggcca agtcatgcta cacagaaagc cttgctcttt
     9421 cccaggagac cggggaacgc attcggcagg cggttcagtt cggcaacctg ggctttactg
     9481 cctaccatca aggccattat caagcggcca tcgagtacat ccagcaaggc ctggtcattg
     9541 tggaggaaat ggagattatc ccggaagtgc ccacctggct atacagtctg gccggcccgg
     9601 tcgccgcact gggctatccc ttgcacgcgg cgcacttgct cggcacggcc gactccatac
     9661 taaataccgt tggcgttgaa ttgcagccgg ccgatcggca tgatgtcctg ccgatcatcg
     9721 agtccgtccg tcgcacttta ggcgaggcgg agtatcagca agcctggcaa gccggatatg
     9781 agttgacgct ccagcaggcc atcgaatttg cgcttggaat tgaattgagg ggggccaagg
     9841 gcaaggaatg agcgtattct ataccgtcat ttcctgcgta tcctgcgaaa agaacaacac
     9901 acgatcgagc attcaaccca aatcaacgcg tcactcgtca ctcaaaaaaa tcggccgccg
     9961 cccccacagg gcgggcaagg gcggcggccg aatcgattag agtcccttaa cgacgctgcc
    10021 ggccgccaac agcacaaagc cgataaccaa cagccaaaag ccgaacccgg ccgatacaat
    10081 gggcaaagac accagattgg ccaaaatgcc gattacgcca agaacaagag cgatcaccca
    10141 ggtgatcatc ttcggtgaac taagggtcat gacaatatcc tcccgcatga atgataagtt
    10201 gataatcgcc cggcgacttc cctcgccctc cggcacctgc cggccgggat gtcgcgtatc
    10261 atgcggcctc aatgaaatga tgagtggggc atgagcggac gacaggaatt ttatcgcgct
    10321 cgggggggat gtcaatacct gagcagaaat acctaaattg taggggcggg tttggacgac
    10381 agactgcctg tcgcacccgc ccctactcaa tgggcgctgc gttgcgcgat cagttcctcg
    10441 accgtcagat gatccagccc ggcgcggcgc agcttctcga tgtagctgcc ggtcacgccg
    10501 ttggcacgga ggcgcaccag gtcgtcggcc gacagatcgg cgtacccggc agccttcatc
    10561 tcggccacga attcgggcgt cacatcgtgg atgctcatgg ccgtcagatc gccggccgac
    10621 agctcggtga agtccatctg gcgcatcatg tcgatcagct cctcatcgac gccgtggatg
    10681 cccatctgca ccagttcatc ggccgtcacg tcggtcagcc cggccttttc gagcatggcg
    10741 agcatctcct cgtccacgcc gtggatgctc atccgcacca ggtcatcggt cgtcaggtga
    10801 tcgaacccca gcttccgtgc ccgccggacg aactcgatgt cgacgccatg gatgcccatc
    10861 cgcaccacgt catcgactgt cagatcgaag cccagatcgc gcatctcgcg gacatactcc
    10921 ggctcgatgc cgtgaatgcc cagggcgatg acgtcgtcga tggcgatgtc gcccagcgcg
    10981 gcgcgcatct tgttcagata ggcggcgtcg ataccctgat tgcgcagccg gatggcctca
    11041 tcgatgacat cggcgcggcg gccgcggccg gtgcggggcg ggatcggagc gcgcggcggc
    11101 acaggcgcgc gcggcggcac gggcggcaca ggcggcacag gcggcacggg ggcccaaccg
    11161 aactcaaacg gcccggccca ttgctcggcc gctttcagcg ccgccatatc gggcgcgggc
    11221 gcggccgact cgctctccgc cagcgcggcc agcagcagct cggcctcatc tatggtcagc
    11281 ttacccatcg acaacaattg caaaacctgc atccgttcag tactcatgtc caatgttctc
    11341 cttgatgttc ctaattcgta atggccttgt tgcatgaatc aaatagtgta agctagaagt
    11401 at
//
LOCUS       DAOMQG010000076        11303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2478_length_11303_cov_8.523560, whole genome shotgun sequence.
ACCESSION   DAOMQG010000076 DAOMQG010000000
VERSION     DAOMQG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2478_length_11303_cov_8.523560"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLC06_06190"
     CDS             complement(<1..368)
                     /locus_tag="PLC06_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRO90070.1"
                     /translation="MTHSGLLVIDKPVGPTSHDVIARVRRAAGTRRVGHAGTLDPLAS
                     GVLLVCVGGATRLVEYLVGLDKVYETTIRLGQTTTTYDAEGEVTAEHPVTAGEADILA
                     ALPAFRGIIRQRVPSYSAVKR"
     gene            complement(365..1345)
                     /locus_tag="PLC06_06195"
     CDS             complement(365..1345)
                     /locus_tag="PLC06_06195"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRO90071.1"
                     /translation="MIQFAIEKASRVLVIAHVDPDGDAIGSLTAVGQGLQQLGKRVTL
                     VCDDAVPPRFLGLSLAGEIRRAPNTETHYDLVIAVDCADQSRMGWAYDSLPDPKPSLA
                     NIDHHITNTRFGAFNIIDPNASSTAELLHRFLPEIGVEMTPGIAESLLTGIVTDTMGF
                     RTTNVTAATLRAAADLVDAGADIAGITLHSLHQKPFTTAKMWGIGLGRMQLEDGLLWT
                     SISREDIQSAGLSGNPGSSGLVNFLADVDAAVMGVVLTESDDGDVRVTMRAQPPYNVA
                     EVAVKFGGGGHALAAGCALAGPLEQAEARLVAACHEAIDRQRALAASSHS"
     gene            complement(1350..1745)
                     /gene="rbfA"
                     /locus_tag="PLC06_06200"
     CDS             complement(1350..1745)
                     /gene="rbfA"
                     /locus_tag="PLC06_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRO90072.1"
                     /translation="MSKIRQERTAEQIRVILSELFLKEVSDPRLEGITVTEASIDREL
                     QHANVYVNALGDESREKEVMDGLQSASGFLRGQVAQRLDLRSAPILHFRWDPRLRYAE
                     EINDLLDNLNIQPTESKPEASPATEPADE"
     gene            complement(1747..3531)
                     /gene="infB"
                     /locus_tag="PLC06_06205"
     CDS             complement(1747..3531)
                     /gene="infB"
                     /locus_tag="PLC06_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRO90073.1"
                     /translation="MADTKTLIEIPAFVTVRELATMMGVSPINVIKELMANGIMANIN
                     QQIDFDTAAIVAGELGFDVVAQQEAVAEAAEDEEKTGWRKVLAEESDANLQPRPPIVT
                     MLGHVDHGKTSLLDVIRKANVTKGEAGGITQHIGAYQTVKDGRLITFLDTPGHEAFTA
                     MRARGAQATDVAILVVAADDGVMPQTREAADHARAAGVPIVVALNKIDLAGARPDRVK
                     QQLSEMGLVPDDWDGDTLVIPVSAREKLGIDDLLEAVLLTAEEVNPRANPEATASGTV
                     LEAKIERGKGIMTTLLVQNGTLRVGDTLLIGEHYGKIKSMFDYTGKNVKAAGPSSPVA
                     VSGLDGIPVAGEQFQVVESEKVARKMIDDAREAARVTPTSRGKSTTLDEFFARLQEGE
                     TKTLNLIVKADVQGSLEPIVTSLERLNGGEVELQILRAATGPITESDVMLASASDAII
                     LGFNVEADPIARNSAAVEEVQIRTYQIIYKLIEDVEKAMKGLLAPTYEDVVIGRAEVR
                     QVFKIRNVGAIAGSYMRTGEGRRNAKARVIRNSRLLHSGPVSSLKHLQDSVREVKAGF
                     EFGVTLDGWNDFQAGDIIEFFVTQRVEQ"
     gene            complement(3613..3936)
                     /locus_tag="PLC06_06210"
     CDS             complement(3613..3936)
                     /locus_tag="PLC06_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRO90074.1"
                     /translation="MTGKQPGRPARHVPQRTCIVCRRQFDKRRLTRVVRTPDSGVVVD
                     PTGKRSGRGAYLCDQVACWDKAIRHPGILNQALNAQLSEAELAAIAADPRRPTATAAT
                     EQVIE"
     gene            complement(3946..4818)
                     /locus_tag="PLC06_06215"
     CDS             complement(3946..4818)
                     /locus_tag="PLC06_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRO90075.1"
                     /translation="MGRPVTAEDYLTLDRLVAGVEGRIIADRAQKFEVVRRERAELRK
                     RVPEEAWQQPLDVLDLPGRIHNLLLDTNVNTVGDLIYILEMGDDYFLKLRGLGEKALE
                     TVKETLTAYQSAQIDALVAAESEAVAEAEVAGLIDDGEFTVVEEVPLIDRVVDAEEME
                     TSVPDMDFADEEVEIMAADEETEEGEIVGLLEPDEVLPEIEETLTPLDDLSQTIFVEE
                     AKPAKPEPKKKPGVVVVRPTAEEAAAEEETKRKKRKGQPLVYNEDLDQVVVQRKRKKG
                     GSDWLDEDIDLSDL"
     gene            complement(4927..5457)
                     /gene="nusA"
                     /locus_tag="PLC06_06220"
                     /pseudo
     CDS             complement(4927..5457)
                     /gene="nusA"
                     /locus_tag="PLC06_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
     assembly_gap    5458..5557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5558..5823)
                     /locus_tag="PLC06_06225"
     CDS             complement(<5558..5823)
                     /locus_tag="PLC06_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HRO90076.1"
                     /translation="MKSEFILAFNEICESRGLPKEDVFEALKIALVSAYRRDANLSSN
                     QMVTVEIDPRTGEPTIFTEKEVVDDIIDNRTEVTLTAARKEGHT"
     gene            complement(6084..7124)
                     /locus_tag="PLC06_06230"
     CDS             complement(6084..7124)
                     /locus_tag="PLC06_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRO90077.1"
                     /translation="MNPQLNEALEEARQPFADTRFNVCRLEIVESDGRRHLEGTVLDE
                     ATLDAVRDHLRRRAPDARWEFGRVRVLRQTSPHLRVVTANLTGWQREPSWLAEQQSQV
                     LNGTVVEIFDEGERWSFGRLEDGYLGWVFNGYLGAANEAAEPNVLVGEPMALMRNGPD
                     AAAPLVGRILAGTRVAAVASWRPEGWMEVTLAGGLRGYVFATELRPLVNDLDPAATRA
                     RLIEDAHRYIGVPYLWGGTSALGIDCSGFAQLMHKLAGVIIPRDADVQFDAGQPVEPP
                     YAAGDLLYFGGSGGHRVVSHVGISLGPARDKDGMVMIHSGRSRNGVYVDDINAVESLR
                     DSFLGGRRFMHP"
     gene            complement(7157..8203)
                     /locus_tag="PLC06_06235"
     CDS             complement(7157..8203)
                     /locus_tag="PLC06_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRO90078.1"
                     /translation="MTHLNWEPITLHLRNPFRISHGTSETRTAHWLRLSGASPADEGW
                     GEGTIPPYYGVSTEMMAAWWDAAARRLDPLPDDPAGIAAFVGSDGPAPARAALDLALH
                     DRLGKRHDKPLHALLGLPTPRPLPTSFTLAIDEPDAMGRSAAELTGCSVIKLKLGGAD
                     GRDVERVAAVRAARPDATLYVDANAGWSADEAIGEIGRMVALGVSLVEQPVAKGDYEG
                     MGRVQAACAVPVVADESLQTLTDVDRLAAAGVGGINLKLMKVGGLAPGLAILRRAREL
                     GLGIMLGCMVETSLGATAMAHLMGLADWLDLDAPWLIANDPFDGLIYDDAGRVHVPDR
                     PGIGVVRREAAATD"
     gene            complement(8285..8959)
                     /locus_tag="PLC06_06240"
     CDS             complement(8285..8959)
                     /locus_tag="PLC06_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO90079.1"
                     /translation="MTIRDNDETLLTRILESPRLPFYARQIQTVLNDEQDARHRFYEA
                     ITEGDKAEFINGEPVFHSPVRLEHNQVGQLLLTLLSTYVRINDLGYVGYEKIMISLSR
                     NDYEPDLCYFRKETAAQFKPRQMHFPAPDFIVEILSESTAAHDRGVKLVDYAAHGVGE
                     YWIIDPTAEVVEQYLLRGDSYELAVKVIDGPLGSAAVPGFHIPVRAIFDETENVRALQ
                     TILQAG"
     gene            complement(9024..10166)
                     /locus_tag="PLC06_06245"
     CDS             complement(9024..10166)
                     /locus_tag="PLC06_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRO90080.1"
                     /translation="MTHTQRLLAHLTNQRHDMVALLAELVAAESPSDVPETQAGPIGI
                     LYEQFDRLGYHVRHTPGTRTGGYLIARHPTAGERPTQLLLGHCDTVWAVGTLAEMPLE
                     IEGDILRGPGSFDMKGGLTQGIFALRALQELGLEPPLAPVFLINTDEEIGSIESRPVI
                     EQLAAESARALILEPAAGAAGKLKTARKGIGDFEIVVRGRASHAGLEPEKGVSAILGM
                     AEIVRALATLNDYPRGVSVNTGLISGGLRSNVVAPECRAVVDVRAATVADAEQLEQAV
                     RALTPPLEGTTLEITGGFDRPPLERTPRNAALWELTRDIGAEMGLALEETAVGGGSDG
                     NLTSLHTATLDGLGPVGDGAHARHEHVVISKMVERSALLAALLMKE"
     gene            10452..>11303
                     /locus_tag="PLC06_06250"
     CDS             10452..>11303
                     /locus_tag="PLC06_06250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRO90081.1"
                     /translation="MTRGVIAAGDRQTAAAGAEILRRGGNAADAAVAAAFVSFIAEAG
                     VVHLGGSGIAQLYDARTGESRVYDFFSVVPGLDGTPPASLDFEEVTIDFGSATQRFYL
                     GRGSVAVPGNIAGLCRLAADYGRLPLDVLLEPALRLARDGVALSAFQADTCRLLAPLY
                     THTDDIRRVYAPHGRLIEAEERLFIPDLANTLTALAREGEAYARDGALARALLDDQAA
                     NGGLLTATDLTRYEVLRRAPITLRYRDYEVMLPGMCSVGGILTAFTLRLLSAFDVAAL
                     PHGSAAHL"
BASE COUNT         1760 a         3572 c         3748 g         2123 t
ORIGIN      
        1 cgcttcacgg ccgagtagga cggcacacgt tgccggatga ttccgcgaaa ggcgggcagg
       61 gcggccagaa tgtcggcctc tccggctgtc acggggtgtt cggccgtcac ttcgccttcg
      121 gcgtcgtagg tcgtcgtcgt ttggcccagc cggatcgtgg tttcgtagac cttgtccaga
      181 ccgacgagat attcaaccag gcgcgtcgcc ccgccaacgc aaaccagtaa cacgccgctc
      241 gccaacgggt cgagcgtgcc ggcatgacct acgcggcgcg tgcccgcagc acggcgcacc
      301 cgcgcgataa cgtcgtggga cgtggggccg acgggcttgt cgatgacgag aagaccggaa
      361 tgggtcacga gtgggaagaa gcggcgaggg cgcgttggcg gtcgatggct tcgtggcagg
      421 ccgcgacgag acgcgcttcg gcctgttcca gcgggccggc gagcgcgcaa cccgcggcga
      481 gggcgtggcc gccgccgccg aatttcaccg ccacctcggc cacgttatag ggcggttggg
      541 cgcgcatggt gacgcgcacg tcgccgtcgt ccgactccgt caggacgacg cccatcaccg
      601 ccgcatcgac gtcggccaga aaattaacga ggcccgaact gccgggattg ccgctcaggc
      661 cggcgctctg gatgtcctcg cgggagatgc ttgtccagag cagaccgtcc tcaagctgca
      721 tgcgccccaa gccgatgccc cacattttgg cggtcgtgaa tggcttctga tgcagcgaat
      781 gcagggtgat gccggcgatg tcggccccgg catccaccag atcggccgcc gcgcgcagag
      841 tggccgcggt cacattcgtc gtgcgaaagc ccatcgtgtc ggtgacgatg ccggtcagca
      901 ggctctcggc gatgcccggc gtcatctcca cgccgatctc cggcaaaaag cggtggagaa
      961 gctcggcggt ggagctggca ttggggtcga tgatattgaa cgcgccgaag cgggtgttgg
     1021 taatgtggtg gtcgatattg gccagcgacg gcttggggtc gggcaaggaa tcgtaggccc
     1081 agcccatgcg cgactggtcg gcacaatcga cggcgatgac cagatcgtaa tgcgtctccg
     1141 tgttgggcgc gcgccggatc tctccggcca atgacagccc caggaagcgc ggcggcacgg
     1201 cgtcgtcgca gaccagcgtc acccgcttgc ccagctgttg cagcccctgg ccgacggccg
     1261 tcagcgagcc gatggcgtcg ccgtcggggt cgacgtgggc gatgaccagc acgcgggacg
     1321 ctttttcgat ggcaaattga atcacgcgct tactcgtccg ccggttcggt ggcggggctt
     1381 gcttcgggct tactctccgt cggctggata ttcaggttgt ccagcagatc gttgatttct
     1441 tcggcgtagc gcaggcgcgg gtcccagcgg aagtggagga tcggcgcgga acgcagatcc
     1501 agccgctggg cgacctggcc gcgaaggaag cccgacgcgc tttgcagccc gtccatgact
     1561 tctttttcgc gcgattcgtc gcccaacgcg ttgacgtaga cgttggcatg ttgcagttcg
     1621 cgatcgattg aggcttcggt gacggtgatg ccttccagcc gcggatcgct gacttctttc
     1681 aggaaaagct cgctcaggat gacccgaatc tgttcggccg tgcgctcttg tctgatcttg
     1741 ctcatcttat tgctcaaccc gctgcgtgac gaagaactcg atgatgtcgc ccgcctggaa
     1801 atcattccag ccgtcgaggg tgacgccgaa ctcgaagccg gccttcacct cgcggacgct
     1861 gtcctgcaaa tgtttcaggc tgctgacggg gccgctgtgc agtaggcgac tgttgcggat
     1921 gacgcgcgcc ttggcgttgc gtcgtccctc gccagtgcgc atgtaggagc cggcgatggc
     1981 gccgacgttg cggatcttga agacctggcg cacctcggcg cggccgataa ccacatcctc
     2041 gtaggtcggg gccagcaagc ccttcatcgc cttttcgacg tcttcgatca acttgtagat
     2101 gatctgatag gtgcgaatct gcacctcttc cacggcggcc gagttgcggg cgatggggtc
     2161 ggcctcgacg ttgaagccca ggatgatcgc gtcggaggcc gaggccagca tgacgtcgct
     2221 ctcggtgatg gggcctgtgg ccgcgcgcaa gatctgcaat tcgacctcgc caccgttcaa
     2281 ccgttccagc gacgtgacaa tcggctccag cgagccttgc acgtcggcct tgacgatcag
     2341 attgagcgtc ttggtctcgc cttcttgcag ccgggcgaaa aactcatcca gcgtcgttga
     2401 tttgccgcga gaggtgggcg ttacgcgcgc cgcttcgcgg gcatcgtcga tcatcttgcg
     2461 ggcgaccttc tcgctctcga ccacctggaa ctgctcgccg gcgacgggaa tgccgtccag
     2521 gcccgacacc gccaccggtg acgacggacc ggccgccttc acgttcttgc cggtgtagtc
     2581 gaacatcgac ttgatcttcc cataatgctc gccgatcagc agcgtatccc cgacgcgcaa
     2641 ggttccgttc tgcaccagca gcgtggtcat gatgcccttg ccgcgctcga ttttggcctc
     2701 gagcacagtg cctgaggcag tcgcttcggg gttggcgcgg gggttcactt cctcggccgt
     2761 cagcagaacc gcttccagca ggtcgtcgat gcccagcttc tcgcgggccg acacgggaat
     2821 gaccagcgtg tcgccgtccc agtcgtcggg aaccaggccc atttcggaca gctgctgctt
     2881 cacgcggtcg gggcgagcgc cggccaggtc gatcttgttc agggcgacga cgatgggcac
     2941 accggcggcg cgggcgtggt cggcggcctc gcgggtctgg ggcatcacgc cgtcgtcggc
     3001 ggccacgacc aggatggcga cgtcggtcgc ctgggcgccg cgggcgcgca tggcggtgaa
     3061 cgcctcgtgg ccgggcgtat ccaggaaggt gatcagccgt ccgtccttca cggtctggta
     3121 ggcgccaatg tgctgggtga tgccgccggc ctcgcctttg gtgacgttgg ccttgcgaat
     3181 cacgtcgagc aacgaggtct tgccgtggtc gacgtggccg agcatggtga cgatgggtgg
     3241 ccgcggctgc aagttggcat cgctttcctc agccaatacc ttgcgccagc cggtcttttc
     3301 ttcatcttcg gccgcttcgg caaccgcttc ctgttgggcg accacgtcga agcccaactc
     3361 gccggccacg atggccgccg tgtcgaagtc gatttgctgg ttgatattgg ccatgatgcc
     3421 gttggccatc aactccttga tgacgttgat cgggctgacg cccatcatgg tggccaattc
     3481 gcgaaccgtc acaaatgccg gaatctcgat taaagtttta gtatctgcca tactgtttgc
     3541 ccactccgtt tgcccactcc gctcgccgtc tttggggcgc atcggcgaaa cccggccgaa
     3601 tgcgccgtgg ctctattcaa tcacttgttc tgtcgcggcc gtcgccgtcg ggcggcgggg
     3661 atcggcggca atggccgcca gttccgcttc gctcagctgc gcgttgagcg cctggttcag
     3721 gatgccggga tggcggatgg ccttgtccca gcatgcgacc tgatcgcaca aatacgcgcc
     3781 acggccgctg cgtttgccgg ttgggtcaac cacgacgccg ctgtcgggtg tgcgcaccac
     3841 gcgcgtcagc cgccgcttgt caaactggcg gcggcagacg atgcatgttc gttgcggcac
     3901 atggcgcgcg gggcggcctg gctgcttgcc ggtcattgtt aattcctata gatcactcag
     3961 atcgatgtcc tcatcgagcc agtcggagcc gcccttcttg cgcttgcgct ggacgacgac
     4021 ctgatccagg tcttcattat agaccagagg ctggcccttg cgcttcttgc gcttggtctc
     4081 ttcctcggct gctgcttctt cggccgtggg tcgcacgacg acgacgccgg gcttcttctt
     4141 cggctcgggc ttcgcgggtt ttgcttcttc cacgaagatg gtctgcgaca ggtcgtcgag
     4201 cggggtgagg gtctcttcga tttcgggcag cacctcgtcg ggttccaata ggccgacgat
     4261 ctcgccttct tcggtttcct cgtcggcggc cataatctcg acttcctcat cggcgaagtc
     4321 catatcgggc accgacgttt ccatctcttc cgcgtctact acccggtcga tcagcggcac
     4381 ttcctcgacg acggtgaatt cgccgtcatc aatcagcccc gccacttccg cttcggcgac
     4441 ggcttcgctt tcggccgcca ccagcgcgtc aatctgcgcc gactggtagg ccgtcagagt
     4501 ctccttaacc gtttccaggg ccttttcacc caggccgcgc agcttcagga agtaatcgtc
     4561 gcccatctcc aggatgtaga tgagatcgcc gacggtgttg acgttggtgt cgagcagcag
     4621 gttatggatg cggcccggca aatcgagcac gtccagcggc tgctgccagg cctcttcggg
     4681 cacgcgcttg cgcaactcgg cccgctcgcg gcgcacgact tcgaatttct gcgcgcggtc
     4741 ggcgataatg cgtccctcga cgccggccac aagccggtcg agcgtcaggt aatcttcggc
     4801 cgtgaccggg cgacccatct gctttttgtc cagaatgttg cgtacttgcg cctggagcgc
     4861 ctcgtccttg gcgacgcgct ggtcggccat cggatgatcg aggttgttga gcgattccgc
     4921 cgccgcctcg gtcaggtttt tgatgtcgat gcgccagccg gtgagcttgg cggccagacg
     4981 ggcgttttgc ccttcgcggc cgatggccag cgagagttga tcgtcgggca cgatgaccac
     5041 ggccgtcttg ccttcttcgg gatgctcttc cagaaagacg tgggagacgc gcgccgggct
     5101 gagcgccttg gcgatgaaca cgcgctggtc gccgtcccat tcgatgacgt cgatcttttc
     5161 gtcgttcagc tcgcggacga tgctctggat gcgcacgccg cgcatgccga cgcacgcgcc
     5221 gaccgggtcg acgccgtgct gcaatgcctg cacggctacc ttggagcgct ggcccgcctc
     5281 gcgggcgatg ctcttgatgt ccacctggcc gttgtagatt tccggcactt ccagctccag
     5341 caggcggcgc agcaggttgc gatggttacg gctgacgacg atctgcgggc cacggctggt
     5401 gcggcgcact tcgagaacgt agacgcgaat cttgtcgtgg gcgcgatagc gctcgccnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg gtgtggcctt ccttgcgcgc
     5581 cgcggtcaac gtcacctcgg tgcggttgtc gatgatgtcg tcgacgacct ctttttcggt
     5641 gaagatggtc ggttcgccgg tgcgcgggtc gatctccacg gtaaccatct ggttactact
     5701 gaggttggcg tcgcgccggt aggcggaaac aagggctatc ttcaatgctt cgaagacgtc
     5761 ctctttgggg agaccccgtg actcacatat ttcgttgaaa gccaggataa attcgctctt
     5821 catggaactt gttcaaccta ttcagttttt gtgcgacggt gcgctatcga atttcacgac
     5881 agacacaaga aacaaaaaaa gtgggggtgc ccacttttcc gtcgatgaat ctccagattg
     5941 tcgtgagaca tagtatagca cagggtgggg caactgacaa ccacgggaag aggttgttgc
     6001 cgggcaacaa tcgtattgga ccgcgagttg ctgcgaatga acgcgaatga acgcgaataa
     6061 gaacggatgg ggcaggtttg aggtcagggg tgcataaacc gccgcccgcc cagaaagctg
     6121 tcccgcagcg actcgaccgc gttgatgtca tccacataca ccccattgcg cgagcggccg
     6181 gagtggatca tcaccatgcc gtccttgtcg cgcgccgggc cgaggctgat gcccacgtgg
     6241 ctgacgaccc gatggccgcc cgacccgccg aagtagagca ggtcgccggc ggcgtagggc
     6301 ggctccaccg gttgcccggc gtcgaactgc acgtcggcgt cgcgggggat gatgaccccg
     6361 gccagcttgt gcatgagctg ggcgaagccg gagcagtcga tacccagcgc cgacgtgccg
     6421 ccccacagat agggcacgcc gatgtagcgg tgggcgtctt cgatgaggcg cgcccgcgtg
     6481 gcggccgggt cgaggtcgtt caccagcggc cgcaactccg tagcgaacac gtagccgcgc
     6541 aagccgccgg ccaatgtcac ttccatccag ccttcgggac gccatgaggc gaccgcggcc
     6601 acgcgcgttc cggccagaat gcggccgacc agcggcgcgg cggcgtccgg cccattgcgc
     6661 atgagggcca tcggctcgcc gaccaggacg ttgggttcgg ccgcttcgtt tgccgcgccg
     6721 aggtagccgt tgaagaccca gccgaggtag ccgtcctcga ggcggccgaa tgaccagcgc
     6781 tcgccctcgt cgaaaatctc gacgaccgtg ccgttgagca cctgcgattg ctgttcggcc
     6841 agccacgacg gctcgcgctg ccagccggtc aggttggcgg tgacgacccg cagatgcggc
     6901 gaggtctgac gcagcacgcg gacgcggccg aattcccagc gggcgtcggg cgcgcggcgg
     6961 cgcaggtggt cgcgcacggc gtcgagcgtg gcctcgtcca gcacggtgcc ttcgaggtgc
     7021 cggcggccgt cgctctccac gatctccagc cggcagacgt tgaagcgggt atcggcaaat
     7081 ggctgtcggg cttcttccag agcttcattg agttgcgggt tcataggtga attgtccatc
     7141 ggatttgttt gtcgtgtcaa tcggtcgccg ctgcttcgcg gcgaactacg ccaattcccg
     7201 gccggtcggg cacgtggacg cgcccggcgt catcatagat caggccgtcg aacgggtcgt
     7261 tggcgatgag ccacggggcg tcgaggtcga gccagtcggc cagccccatc aggtgggcca
     7321 tggccgtcgc gccgagcgag gtctcgacca tgcagccgag catgatgccc agccccagtt
     7381 cgcgcgcgcg gcgcaggata gccagcccag gcgccagccc gccgaccttc atcagcttga
     7441 ggttgatgcc gccgacgccg gcggcggcca gtcgatccac gtcggtcagg gtctgcagcg
     7501 actcgtcggc gacgaccggc acggcgcagg ccgcctgcac ccggcccatg ccttcgtagt
     7561 cgcccttggc aacgggctgc tccaccagcg acacgccgag ggccaccatg cggccgatct
     7621 cgccgatggc ttcgtcggcc gaccagccgg cgttggcgtc gacgtagagc gtggcgtcgg
     7681 ggcgggcggc gcgcacggcg gccacgcgct ccacgtcgcg gccgtcggcc ccaccgagct
     7741 tgagcttgat gaccgagcag ccggtcaatt cggcggcgct gcgacccatc gcgtccggct
     7801 cgtcgatggc cagcgtgaac gatgtgggca gcggccgcgg cgtgggcagg cccagcaggg
     7861 cgtggagcgg cttgtcatgg cgcttgccga ggcggtcgtg gagcgccaga tcgagcgcgg
     7921 cgcgggccgg ggcggggcca tcgctgccca cgaaggcggc gatgccggcc ggatcgtcgg
     7981 gcagggggtc gaggcgacgg gcggccgcgt cccaccaggc ggccatcatc tcggtggaga
     8041 caccataata gggcggaatg gtgccctcgc cccagccctc gtcggcgggc gaggcgccgc
     8101 tcagccgcag ccagtgggcg gtgcgcgtct cgctggtgcc gtggctgatg cggaacgggt
     8161 tgcgcaggtg cagggtgatc ggttcccagt taagatgagt catggagcga tattatccgt
     8221 gaattttgcc gattgggcag atttatggag cctgcaatcg gccgaacggg tgggagctgc
     8281 ggccttatcc ggcctgcagg atggtttgaa gcgcccgcac gttttcggtt tcgtcgaata
     8341 tggcgcgcac ggggatgtgg aagccgggca cggccgcgct gccgagcggc ccatcgatga
     8401 ccttgacggc cagctcgtag gagtcgccac gaagcaggta ttgctccacg acctcggccg
     8461 tcgggtcgat gatccagtat tcgccgacgc catgggcggc gtaatccacc agcttgaccc
     8521 cgcgatcatg ggccgccgtg ctttcggaga ggatttccac gatgaaatcg ggcgcgggaa
     8581 agtgcatctg gcgcggcttg aactgggcgg ccgtttcctt gcggaagtag cacagatccg
     8641 gctcgtagtc gttgcgcgag agggagatca tgatcttctc gtagccgacg tagcccagat
     8701 cgttgatacg gacataggtt gacagcaatg tcaagagaag ttgcccgacc tgattgtgtt
     8761 ccaggcgcac aggggagtgg aaaaccggct caccgttgat gaactccgcc ttgtcgcctt
     8821 cggtgatcgc ttcgtagaag cgatggcgtg catcctgttc gtcgttgagg acggtctgga
     8881 tttgccgcgc gtaaaatgga agccgggggg attccaggat tcgcgtcaga agggtctcat
     8941 cgttgtctcg gatcgtcatc gcagctgcct tcatcaagga ttgaatacca gtataatgga
     9001 cgaccggatc gttcgcaagg cggctattcc ttcatcagca gcgcggccag cagggcgctg
     9061 cgctcgacca ttttggagat gacgacgtgc tcgtggcggg catgggcgcc gtcgcccacc
     9121 gggccgaggc cgtcgagcgt ggccgtgtgc aggctggtca gattgccgtc agagccgcca
     9181 ccgacggccg tctcttccag cgccagcccc atctccgcgc cgatgtcgcg tgtcagttcc
     9241 cacagcgcgg cgttgcgcgg ggtgcgttcc agcggcggcc gatcgaagcc gccggtgatc
     9301 tccagcgtcg tgccttccag cggcggggtc aaggcgcgca cggcctgttc cagctgctcg
     9361 gcgtcggcca cagtggcggc gcgcacgtcg accaccgccc ggcattccgg agcgacgacg
     9421 ttggagcgca gcccgccgct gatgaggccg gtgttgaccg acacgccgcg cgggtaatca
     9481 ttgagcgtcg ccagcgcgcg gacgatctcg gccatgccca gaatggcgct gacccccttc
     9541 tccggctcca gcccggcgtg ggaggcgcgg ccgcggacga cgatctcgaa gtcgccgatg
     9601 cccttgcgcg ccgtcttgag cttgcccgct gccccggccg ccggctccag gatgagcgcg
     9661 cgggccgatt cggcggccag ctgctcgatg accggccgcg actcgatgct gccgatctcc
     9721 tcgtcggtgt tgatgaggaa aacgggcgcg agcggcggct ccagccccaa ctcctgcaag
     9781 gcgcgcaggg cgaagatgcc ctgcgtcagg ccgcccttca tgtcgaagct gccggggccg
     9841 cgcaagatgt cgccctcgat ctccagcggc atctcggcca gcgtgccgac ggcccagacg
     9901 gtgtcgcagt ggccgaggag gagttgcgtc ggccgttcgc cggcggtcgg atgacgggcg
     9961 atgagatagc cgccggtgcg cgtacccggc gtgtggcgga cgtggtagcc gaggcggtcg
    10021 aactgctcat agaggatgcc gattggtccg gcctgcgtct ccggcacgtc ggacggcgat
    10081 tcggcggcga cgagttcggc cagcagggcg accatgtcgt ggcgctggtt ggtcaggtgg
    10141 gcgagaaggc gttgggtgtg ggtcatgggg gaagtatagc caaaccggat acgagatacg
    10201 agatgcgaga tacgaattac gaattaagag cgtcactcgt cactcccacc caaagcgccc
    10261 cctagcgagg tatcgttccg cggattggaa accgggtttc ttccgggaaa cccggcttct
    10321 actcttcccg ttaatcaatt tggcgtccat gtggccccac cccccagggt ttttcagtac
    10381 gttgaatggg gcaataaaca agcggatggg ttaaactact cctcttaatc cgttagtaaa
    10441 gaggaagcgt tatgaccaga ggcgtaatcg ccgccggaga ccgacagacc gccgccgccg
    10501 gagccgaaat attgcgacgg ggcggcaatg ccgccgacgc ggccgtggcc gctgccttcg
    10561 tgtcgtttat cgccgaggca ggggtcgtcc atttgggcgg cagcggcatc gcccagctgt
    10621 atgacgcgcg gacgggcgaa tcccgcgtct acgacttttt cagcgtcgtg cccgggctgg
    10681 acgggacgcc gcccgcctcg ctcgacttcg aggaagtgac catcgacttt ggctcggcca
    10741 cgcagcgctt ctacctgggc cgtggctcgg tggccgtgcc gggtaatatc gccggactgt
    10801 gtcgcctggc ggccgattac ggccgcctgc ccctcgatgt attactggag cctgccttgc
    10861 gcctggcgcg cgacggcgta gccctgtccg cctttcaggc cgacacctgt cgcctgctgg
    10921 ccccactcta cacccacacc gacgacatcc gccgcgtcta tgccccccac ggccggctca
    10981 tcgaggccga ggagcggctg ttcatccccg acctggcgaa tacgctgacc gcgctggctc
    11041 gcgagggcga ggcctatgcc cgcgatggcg ccctggcccg cgccttgctc gacgaccagg
    11101 cggccaacgg cggcctgctg acggccaccg acctgacccg ctacgaagtc ctgcgtcgcg
    11161 cgcccatcac gctgcgctac cgcgattacg aggtcatgct gccggggatg tgttcggtcg
    11221 gcggcatcct gacggcgttc acgctgcggc tgctgtcggc gttcgacgtg gccgcgctgc
    11281 cccacggctc ggccgcccac ctg
//
LOCUS       DAOMQG010000077        11233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2496_length_11233_cov_11.065844, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000077 DAOMQG010000000
VERSION     DAOMQG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2496_length_11233_cov_11.065844"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..862)
                     /locus_tag="PLC06_06255"
     CDS             complement(47..862)
                     /locus_tag="PLC06_06255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90082.1"
                     /translation="MLRTRAQKRFIAILALTFISTLVVYGVAYLVFQPLATGVVVAWA
                     ASITVLFIYLERRRSGILGLEDQYSDLQSYINLQSVLGETFVPYSYWAMEPASLLNLL
                     ATIQYRDYRTIVECGAGLSTILIGKLLKQLGGGHIYSLEDDERWHAVMSAAVTKEDLA
                     EYITLLHAPLEKNSESGELWYGQAAAQQILDKADHIDLLIVDGPKSIAPLSRMPALPM
                     FVSRLDDDSLIVLDDSKRESEQQVLRQWKERFNLKIEPKRDSLKGQAYIRIIQ"
     gene            complement(881..>1348)
                     /locus_tag="PLC06_06260"
     CDS             complement(881..>1348)
                     /locus_tag="PLC06_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRO90083.1"
                     /translation="AEEIEIEVDEKDLRIDRFRASGAGGQHVQKNETAVRITHTPTGL
                     VVSCQNQRSLTQNTQVAMNILKSQLYDLARRTQNAEIAALKGEDVDAAWGSQIRSYVL
                     QPYKMVKDHRTGHEVGNTQAVLDGRLDDFMEAYLRRQVGDGTDTLESVDAVTE"
     assembly_gap    1349..1448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1449..1826)
                     /locus_tag="PLC06_06265"
                     /pseudo
     CDS             complement(1449..1826)
                     /locus_tag="PLC06_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_102136549.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            complement(1902..2807)
                     /gene="ftsY"
                     /locus_tag="PLC06_06270"
     CDS             complement(1902..2807)
                     /gene="ftsY"
                     /locus_tag="PLC06_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRO90084.1"
                     /translation="MFKSIRESLSRTRQSVFGQIAGVLGAGDITEETWEDLEALLIQA
                     DVGVPTTVYLVDTLREQARREGLYRADQLLDALRGQLRAILVDPPTFEMEEKRLLTVV
                     MVVGVNGSGKTTTIGKLANRYKDVGYKVMLAAGDTFRAAAIDQLTIWGQRADVPVIAG
                     QPGGDPAAVAYDGIRAARARGYNMLFIDTAGRLHTKFNLMKELEKVYGVCHKSVHAAP
                     HDVLLVLDAPTGQNALTQAAKFKESVHVSGVILTKLDSTAKGGMVFAIYRELGLPVRF
                     IGTGERIQDLAPFDADQFIQGLFDQ"
     gene            complement(2857..3084)
                     /locus_tag="PLC06_06275"
     CDS             complement(2857..3084)
                     /locus_tag="PLC06_06275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRO90085.1"
                     /translation="MIAEIEALSFEEALLELEQTVAQLESGHLTLDDSLGLFDRGRYL
                     AAHCGALLDKAQLRIEQLTEDGEIITINPPN"
     gene            complement(3192..5525)
                     /locus_tag="PLC06_06280"
     CDS             complement(3192..5525)
                     /locus_tag="PLC06_06280"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRO90086.1"
                     /translation="MEQIFRFLEMNLTSFEVIATWLVLAIAFVGLLYALFLRRQILAE
                     DKGTEKMVEVWEAIRQGANAYLTRQLRTILPFIVVLTIALFLSVYIVPPTEFSLEVFA
                     GYSEDTIRLIVGIGRAVAFVMGAGFSLAVGQLGMRMAVEGNVRVANAARTSFKDALRI
                     AYRTGTITGMLTDGLGLLGGTLIFMIFGKAAPDALLGFGFGGTLIALFMRVGGGIYTK
                     AADVGADLVGKVEHDMPEDDPRNAAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLI
                     LGVTLTLITGHNEYILYPLLVRGVGVLASIVGTYLVKGESDDSGNAMKAIFKGFLTSA
                     AISVVLFGIIALFYMDGVPGGWWRPFLSTATGVALAIVIDRLTDYFTGTHYRPVKEIR
                     DSTSGGPATTILSGLASGYESAVWSVIVIAATIVAAILIYTGIPADEFVSARFSHDFI
                     QFTFVLYGIAMTGIGMLTLTGNNVAMDSFGPISDNANGIGEMAGLEPKARQIMADLDA
                     VGNTTKAITKGIAIGSAVIAAVSLFGSFITDVSLVDPEALANGIRVSSPLVFVGMLLG
                     GALPWMFSSLAIRAVSRAAGQMVDEVRRQFRIPGILEGTKKPDYARAVTISTIAAQRD
                     LINLAILAVVTPIAVGLLLQVEALGGFQAGIIVSGMLLAVFMSNTGGAWDNGKKLIED
                     EERNIEANTGKGSERHKAAVVGDTVGDPLKDTAGPALNPMIKVVNLVSLIIAPIIVRY
                     SERTLGVLLVSVVLIAILGWAVMRSKQEAPAIGASKA"
     gene            5933..6967
                     /locus_tag="PLC06_06285"
     CDS             5933..6967
                     /locus_tag="PLC06_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRO90087.1"
                     /translation="MATILTYAPAPGHTKAQHPESFSRMTGLIPALEQHGLLADLSQL
                     PPTPATVEQLRRVHTNELIEYVRQVSARGGGLLDSGDTYVTGESFSLASLAAGSCTAA
                     VDRIMTSQAQNGFALIRPPGHHAETDRVSGFCIFNNVAAAARHAQVAHKAKRVAIVDY
                     DVHHGNGTQDIFFEDDSVLFISVHLYAPYFYPGIGGMYEIGVGRGRDYTLNIPLPPYV
                     GDKGYHRVFDELIEPRLKLFQPELILVSAGFDAHWQDPLAMGGLSLTGYAGITRKLLG
                     YAHQLCEDRILFVLEGGYHHEVLSTSVVNVFHALLDHDEILDPIGPMPRPEHDIEALV
                     ERLKAHYLLK"
     gene            7675..9729
                     /locus_tag="PLC06_06290"
     CDS             7675..9729
                     /locus_tag="PLC06_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90088.1"
                     /translation="MAAIEPVTEEITFAEEAQPEPAPFESGADYPDETTPVSSIASVT
                     MGLDEILRSEHPTKDIAELVSYQLGDEDEPLDDLDAAMAWIEELAASQNAPIEEMPSV
                     ADRALASKLMMEAGLTPTVSPLDELGSDSDFVDGLTPPHPFIEEEDYADTVVLIETLT
                     ADQGLTYTPPSEQAAITRTEVEADLSDPLQSAESQDIVTQVDEAIALEEVPEELSFEE
                     AMAFLDEIAEAQPSGEGIAVEAIPTELVGVPAADPVMDEILPESVAPEHHEVAPTEII
                     DNVISLESEDEPEPLSAEDVPWLDTPAEAMVVVSPIDKTMGEDTKRPHVEGLNGTGHG
                     ELEMALLSLDALALPPGKTLDDVAAKLTTARMAPWRNMDSALDWLEQTLIEETPPAVE
                     IADDLDDASLIEQMPEDPDAVLAWLEQIAAQEDAQAAEIDIIPEKTSTVIVDQVQSEP
                     VIEELVEADLLDMPDDPDEVMAWLESLAHGGHPTPPTAERQVAADAPQPEDFVEAAAV
                     VEAIAEEEMAAEPETARIDTHALDDQSPEEPEIEIFIDQEVVTLDTDVEDHVEGSLEE
                     PVQSERATTEGTETVGDAVFVESPITEETVDDVPIADDIIVLDEQEIEAEAAPAGLPE
                     QEAEAIELVDSVVVDIDDTIVATEEILVEELNELIDEESAATLEDELQLPSWLDLLRP
                     LD"
     gene            9780..10229
                     /gene="ndk"
                     /locus_tag="PLC06_06295"
     CDS             9780..10229
                     /gene="ndk"
                     /locus_tag="PLC06_06295"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRO90089.1"
                     /translation="MEQTLVLIKPDGVQRGLVGQIITRLERRGLKLIGMKFMQVSTEL
                     ASTHYEAHVGKPFYEGLVNYITSSPIVAMVWEGKDAIQIVRTTMGATNAAVAAPGTIR
                     GDFAVEIGRNLVHGSDGLESAAREVSLFFKSDELVNWKRETDRWIRE"
     gene            complement(10276..10659)
                     /gene="rsfS"
                     /locus_tag="PLC06_06300"
     CDS             complement(10276..10659)
                     /gene="rsfS"
                     /locus_tag="PLC06_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRO90090.1"
                     /translation="MPASILKYWRCCLEGLELTYLIVDALQDKKGIDILVLDLHSQAV
                     FADFFVICSGESEPQLRALMRAVQIEAKEKGGRMPKGVEGTPADGWILIDYGDVVVHL
                     FDEEKRAYYDLESLWHKGRVVVRMQ"
     gene            10758..>11233
                     /gene="xseA"
                     /locus_tag="PLC06_06305"
     CDS             10758..>11233
                     /gene="xseA"
                     /locus_tag="PLC06_06305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRO90091.1"
                     /translation="MFPFLNQPAREDRPAWSVSELTTYIREMFELDYRLQEIEVMGEI
                     SNFTRAASGHLYFTLKDGAAQIKCVMWRSQAERLRFRPAEGDAVLARGRVSVYDAGGV
                     YQLYAERLQPAGRGDLAVAFERLKEDLAGEGLFDAEFKQPIPRLPRKIGIVTSADAA"
BASE COUNT         2611 a         3238 c         3045 g         2239 t
ORIGIN      
        1 ttaatcaagg tgtttgttaa gacgttcctc gtataagaca agctgttcat tgaatgatgc
       61 gtatgtaggc ttgacctttc agactatctc tctttggttc gatttttaga ttgaatcgtt
      121 ctttccattg cctgagcacc tgttgctcgc tctcgcgctt gctgtcatcc agcacgatca
      181 aactatcatc atcaagcctg gatacaaaca tcggtaaggc gggcatcctg gataaaggag
      241 caattgactt ggggccatcg actatcaata aatcgatgtg gtctgcctta tcgagaattt
      301 gttgggcggc cgcttgaccg taccacaatt ctcccgattc ggagtttttc tccaaaggag
      361 cgtggagaag cgtgatatat tcagccaaat cctccttcgt cactgccgcc gacatgacag
      421 catgccatcg ctcgtcgtcc tccaacgaat agatatgccc tccacccaat tgtttgagca
      481 acttccctat taatatcgtc gacaggcccg cgccgcattc cacaatcgtc cgataatccc
      541 ggtactgaat cgtcgccaac aggttgagca agctcgccgg ctccatcgcc caatatgaat
      601 aggggacaaa ggtctcgcca agcacagact gcaagtttat gtaggattgc aggtcgctgt
      661 actgatcctc aagaccaagg atacccgagc gcctgcgttc cagatagatg aacaggacgg
      721 taatcgaggc tgcccaggca acaacgacac cggttgccaa cggctgaaaa accagatagg
      781 caacgccata gacaaccagc gttgatataa acgtcaatgc aaggatggct ataaatcgtt
      841 tctgcgctct ggttctcagc aattttcaga tccccgcatc ttactcggta acggcgtcaa
      901 cactctccag agtatctgtc ccatcgccca cctgacggcg caaatatgct tccatgaaat
      961 catccaggcg gccgtcaagc actgcctgcg tattgccgac ctcatggccg gttcgatgat
     1021 ccttcaccat cttgtatggc tgcaagacat aggaacgaat ctgactaccc caggcggcgt
     1081 caacgtcttc gcccttgagc gcggcaatct ccgcattctg ggttcgtctg gccagatcat
     1141 agagctgcga cttcaggatg ttcatcgcca cttgcgtgtt ctgcgtaagc gaacgctgat
     1201 tttggcagga aactacaagc cccgtcggtg tgtgggtaat gcgcacggcc gtctcgtttt
     1261 tctgcacatg ctgcccgccg gcaccactgg cgcgaaaccg atcgatgcgc aaatccttct
     1321 cgtcgacctc gatctcgatc tcctcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnntg gatggcgaga atagcaccct cgtcatcatg atcacccgac atcagggttt
     1501 gaaattcgag ctgctctacc tcagcagcca ggcgctccgc ctcggccgcc agttcctcct
     1561 gaagttcgtc atcacccagc tcggccagct ccagcgtgtc ggtcaacgat cggctaatgg
     1621 tctgccacac gacgacctgc tcccgcaggc gagtcaactc gcgcatgacg ccctgaccct
     1681 gcgtcggatc gctccagaag tccgcgtcgg caatcttcgc ttccagttcg gcaatacgtg
     1741 cttccttggc cgctacgtca aagacgcccc cggagcaaat caatgatatg ggtcagctca
     1801 gtcagttgat tgattaattc ttccatgatg cgcgtttctc ttcctgccgg atagtcgctc
     1861 gtggtggctc gtcaattgtc gcccccacgt cgtaaattcg attattgatc aaaaaggccc
     1921 tggataaact ggtctgcgtc aaacggggcc aggtcctgga ttcgctcccc cgtgccgata
     1981 aaccgcaccg gcagccccag ctcgcggtaa atggcaaaca ccatgccgcc tttggccgtg
     2041 ctgtccagct tggtcaggat aacgccacta acatggaccg actccttgaa cttcgcggcc
     2101 tgggtcaaag cgttctgccc tgtgggcgca tccagcacca gcaaaacatc gtgcggcgcg
     2161 gcatggacgc tcttatgaca gacaccatag accttttcca actccttcat cagattaaac
     2221 tttgtgtgga gccggccggc ggtgtcgata aacagcatgt tgtaaccccg cgcgcgagct
     2281 gcccgaatac cgtcataggc cacggccgcg gggtcgccgc ccggctgccc ggcaatcacc
     2341 ggcacatccg ctcgctggcc ccagatggtt agctggtcaa tggccgcggc gcggaacgtg
     2401 tcgccggccg ccagcataac cttgtagccc acatctttat aacgattggc cagcttgccg
     2461 atggtagtcg tcttgccgga gccattgacg ccgacgacca tgacaaccgt cagcaggcgc
     2521 ttctcttcca tctcgaaggt cggcggatca accagaatcg cccgcaactg cccgcgcaag
     2581 gcatccagca attgatcggc gcgataaagc ccttcgcgac gcgcttgctc gcgcagggta
     2641 tcgaccagat aaacggttgt cggcactccg acatcggcct ggatgagcaa agcctccagg
     2701 tcttcccagg tttcctcggt aatatccccc gcgcccaaaa cgccggcaat ttgaccgaag
     2761 accgactggc gggtgcggct tagcgactcg cgaatgctct taaacaaggc aacctcaata
     2821 cttgtaatgc gtgaattcgg aaaaagatga gcggggtcaa tttggcgggt tgatggtgat
     2881 gatctcgccg tcttccgtca actgctcaat ccggagttgc gccttgtcga gcagcgcccc
     2941 acaatgcgcc gccaggtatc gcccgcgatc gaacaggccc agggaatcat ccagagtcaa
     3001 atgaccggac tccagttgag ctacggtctg ctcgagttcc agcaacgctt cttcaaacga
     3061 caaagcttca atctctgcga tcatgtggcc gattgtagtc caaaacgacg cgatctacca
     3121 gtgaaacaaa aagggtggag atccgtcaag acctccaccc tgctgataaa agattcggct
     3181 acgtttgccg cttatgcttt cgaggcacca atcgccgggg cctcctgctt cgagcgcatg
     3241 acggcccaac ccaggatagc gatgagcacc acactcacga gaagaacacc aagggtccgc
     3301 tcactatagc gaacgatgat gggcgcgatg atgaggctga caaggttcac caccttgatc
     3361 atcgggttca aagccgggcc ggccgtatcc ttcaagggat cgccgaccgt gtcgccaacg
     3421 acggccgcct tgtgacgctc cgagcctttg cccgtattgg cttcgatatt gcgctcctca
     3481 tcttcgataa gcttcttgcc gttgtcccag gcaccaccgg tgttggacat gaagacggcc
     3541 aggagcatgc cggagacgat gatccccgcc tggaacccac cgagcgcctc tacctgcaaa
     3601 agcaagccga cggcgatagg agtgacgaca gccaggatcg ccaggttgat caggtcgcgc
     3661 tgggcggcaa tcgtggagat cgtgaccgcg cgggcatagt cgggcttctt ggtaccctca
     3721 aggatacccg ggatgcggaa ctggcgacgg acttcatcga ccatctggcc ggcggcgcgg
     3781 ctgacggcgc gaatggccag tgacgagaac atccacggca gcgcaccacc caacagcata
     3841 ccgacgaaga cgagcggcga agaaacgcgg ataccgttgg ccaatgcctc cgggtcgaca
     3901 agcgacacgt cggtgatgaa cgagccaaac agcgacacag cggcgatgac cgccgagccg
     3961 atggcgatac ccttggtgat agccttggtc gtgttaccga cagcatccag gtcggccata
     4021 atctggcgcg ccttcggctc cagaccggcc atctcaccga tgccgttggc attgtcggag
     4081 atcggcccga aggagtccat ggccacgttg ttgcccgtca gcgtcaacat gccgatgccg
     4141 gtcatggcga tgccatagag cacaaaagtg aactgaatga agtcatgcga gaaccgcgcc
     4201 gacacgaact catcagccgg gatgccggtg tagatcagga ttgcggccac gatagttgcg
     4261 gcaataacga tgaccgacca cacggccgac tcatagcccg atgccaggcc ggagaggatc
     4321 gtcgtggccg ggccaccgga cgtgctgtcg cgaatctcct tgaccggccg gtaatgggtt
     4381 ccggtgaagt aatcggtcag gcggtcaatg acaattgcca aagcgacacc cgtggccgtt
     4441 gagaggaacg gccgccacca cccaccgggc acgccgtcca tatagaacag ggcaatgata
     4501 ccgaagagaa ccaccgagat cgccgccgaa gtgaggaatc ccttgaagat tgccttcatg
     4561 gcattgccgc tgtcgtcaga ctcacccttg acaaggtacg tgccgacgat ggacgccagg
     4621 acgccgacac cacgcaccag caacggatac agaatgtatt cattatgccc ggtgatgaga
     4681 gtcaaggtca cgcccaggat caagccggag acaatggtga cctcataaga ctcaaaaata
     4741 tcggccgcca taccggcgca gtcgcccacg ttgtcgccaa ccaggtcggc gataactgcg
     4801 gcattacgcg ggtcatcctc cggcatgtcg tgctcgacct tgccgaccag gtcagcgccc
     4861 acgtcggccg ccttggtgta gatgccgccg ccgacacgca tgaataacgc gataagtgtt
     4921 ccgccaaagc cgaagccgag caatgcatcc ggggccgcct taccaaagat catgaagatc
     4981 aacgtgccgc ccagcaagcc caggccatcg gtgagcatgc cggtgatcgt cccggtgcga
     5041 taggcgatgc gcagcgcgtc cttgaagctg gtgcgggccg cattggctac acgcacattg
     5101 ccttccacag ccatgcgcat acccaactga ccaacggcca gggagaatcc ggcacccatg
     5161 acgaacgcta cggcgcggcc gatgccgacg atgagccgga tcgtatcttc gctatatccc
     5221 gcgaaaactt cgagggaaaa ctcggtcggc ggcacgatgt agacactaag aaatagagcg
     5281 attgtcagga cgacgatgaa tggcaaaatc gtgcgaagct ggcgagtcag gtaggcgttg
     5341 gccccttggc gaatcgcctc ccagacttcc accatttttt ccgtaccttt atcctcagcg
     5401 aggatttgcc ggcgcaagaa caaggcgtag agaagaccca caaaggcaat cgccagcacg
     5461 agccaagtgg cgatcacctc aaatgatgtg aggttcatct ccaggaatcg aaatatctgt
     5521 tccatactaa ctatcagacc tcctatcggt acaaattaaa atgaagcgac aagtttgatg
     5581 aagtcgccaa ttatatccat gagcacggat catgcgctgt gtcgattctt ctccggccca
     5641 cggcattaac cataaagctg gttacagcga gcaaagcata actgcgttac gaaccctctg
     5701 cctcttgccc attttggaca attaatttca tgccgattca tacaatccgc agtaaagcac
     5761 ataaacatag cctgtctata aaattagaca aatttttgat cataattgcc gggtatcttc
     5821 cggccgatga cgggatggca tgggatcgaa ctgcggtaat cgtggatatt taggcggcgc
     5881 ctttgcactt tcgagtataa tcagcccgat tccgactgcc gtattgagat caatggcaac
     5941 cattctgaca tacgcgcccg ctcccggcca cacgaaagcg caacacccgg aaagcttttc
     6001 acgaatgacc gggctaatcc cggctctcga acaacatgga ttgctcgccg atctgagtca
     6061 attgccgcca acaccggcta cggtggaaca attgcggcgc gttcatacca acgagttgat
     6121 agagtatgtc cggcaggtct ctgcgcgagg cggcgggctg ctggatagcg gggacaccta
     6181 cgtgaccggc gaaagcttct cgctggcaag cctggcggct ggttcctgca cggcggccgt
     6241 agaccggatc atgaccagcc aggcccaaaa cggctttgcc ctgattcgtc cgcccggcca
     6301 ccatgccgag acggatcggg tgagtggttt ctgcatcttc aacaatgttg ccgcggccgc
     6361 ccgtcatgct caagtagccc acaaggccaa acgagtagca atcgtcgact acgatgtgca
     6421 ccatggtaac ggcacccagg acatcttttt cgaagatgat tctgttctct tcatctcggt
     6481 tcacctctac gcaccttatt tctatccggg tattggggga atgtatgaga tcggggttgg
     6541 tcgcggacga gattatacgc ttaacatccc actcccgcct tatgtaggtg ataagggtta
     6601 tcatcgcgtg ttcgatgagc tgattgagcc acgtctcaaa ctctttcagc cggaactcat
     6661 tctcgtatcg gccgggttcg atgcccattg gcaagaccct ctggcaatgg gcgggttgag
     6721 cttgaccggc tatgccggga tcacacgcaa gctgctcggc tatgcccatc agctttgcga
     6781 agatcgcata ctattcgtcc ttgagggtgg ctaccaccac gaagtcctgt ccacgagtgt
     6841 tgtcaatgtc tttcacgcgc tgctcgacca cgacgagatc ctcgatccca tcggccccat
     6901 gcccagaccg gaacacgata ttgaggccct ggtggagcgc ctgaaagccc attacttgct
     6961 aaaataggcc attcgctcga taatatggca tataagttga ccataaattg tcgatcgggc
     7021 aggtttcatc gaatctgggc aggatgtatg catgcgctat ttgggggtgt gacgaaacag
     7081 gactgtcgcc agcgcattca ttaaggaagt gttatggacg atcataccga tgaccgcaac
     7141 cccgaccccc caccggtcgg tacgccggaa gaattggaag aagcgctgaa atggctggaa
     7201 gatatgacag ccaggcaggg ccaacccgcc gacaaatcgg ctgccgcacc ggcgacccct
     7261 ctcgattcac cctttcgggg actaatcgac agcgaagatg gcgatctgcc ggactggctg
     7321 cgcgaacttc ccgtctcgca gcaattgaat gataacgagc cggaaagccg tctcgattgg
     7381 ctggccagaa tggctcagca ggaatctatc gaagaactgc ctacattaga gtggcggcga
     7441 ctttccgagc cggcccaaag cgccatcctg ggcggtcaaa cggccgacat gccgacggaa
     7501 gtttaccccg gcccatcccg tcatgaagaa ccggaacccg gatctactac tgaagaatca
     7561 ccgatcagct ctctggatga agtatcgctg gaatcggacg atcagccgtt ggtcgaagag
     7621 ccgttggcgt cgacgacagt cgatgaatcg gtagaggtgg tcacagaagc gccattggcc
     7681 gcgattgaac cggtaaccga agagataacc ttcgctgaag aagcacagcc cgaaccggcg
     7741 cctttcgagt caggcgcaga ttacccggat gaaacaacac ccgtctcatc aatcgcgtcg
     7801 gtgacgatgg ggcttgatga aatcctcaga agtgagcacc caacgaagga catcgccgaa
     7861 ctggtctcat atcaactggg cgacgaggac gagccacttg acgatctgga tgcggcaatg
     7921 gcctggatag aagaactggc cgccagccag aatgcgccga tcgaggaaat gcccagtgtt
     7981 gccgatcggg cacttgcttc caaactgatg atggaggcag ggctgacgcc taccgtctcc
     8041 ccgcttgatg aactggggtc tgactctgac tttgttgatg gtctgacgcc gccccatccc
     8101 ttcattgagg aagaagacta tgccgatacc gttgtgctga ttgagaccct cactgccgat
     8161 cagggcctca catatacgcc accttcggaa caggcggcta tcacaagaac cgaggtcgaa
     8221 gccgacctca gtgatccgct acaatcggca gaatcgcaag atattgtgac tcaagttgac
     8281 gaggccatcg ccctggaaga agtgccggaa gaactatctt ttgaagaggc gatggccttc
     8341 cttgatgaaa tcgcggaggc gcaaccttct ggcgagggga ttgccgtcga agccattccc
     8401 acagaacttg tgggggtacc ggcagccgat ccggtaatgg acgagattct gccggaaagt
     8461 gtggcgcccg agcatcatga agtagcccca acggaaatca ttgataatgt gatatcgctc
     8521 gagagcgaag atgagcctga gcctctgtcg gccgaagatg tgccatggct cgatacaccc
     8581 gccgaagcaa tggtcgttgt atcaccgata gataaaacga tgggcgaaga cacgaagcgg
     8641 cctcatgttg aaggcttaaa cggcaccggc cacggcgaac tggaaatggc cttgttgagc
     8701 ctcgacgcgc tcgccttgcc acccggcaaa acgctcgatg acgtggccgc caagctgacc
     8761 accgcacgga tggccccgtg gcgcaacatg gactcggccc ttgactggct ggaacagacg
     8821 ctcatcgaag aaactcctcc ggcagtcgag atcgcggacg atctggatga tgcgagtctc
     8881 atcgagcaga tgcccgagga ccctgatgca gttttggcct ggctggagca gatcgccgct
     8941 caggaagacg cccaagcggc cgagatcgac atcatcccgg aaaaaacatc aaccgtcatt
     9001 gtggatcaag ttcaatcaga acccgtcatc gaggaattgg ttgaggcaga cctcctcgat
     9061 atgccggatg acccggacga ggtgatggcc tggctcgaga gcctggcgca cggtggccac
     9121 cccacaccac ccacagctga gcggcaagta gcagccgatg ctccccagcc ggaggatttc
     9181 gtcgaggccg cggcagttgt tgaggcaatc gctgaagaag agatggcggc cgaaccggag
     9241 accgcaagga tcgacaccca cgctcttgac gatcaatcac ccgaagagcc ggagatcgag
     9301 atctttatcg atcaggaagt cgtgaccctg gatacggacg tcgaagacca cgttgaggga
     9361 tcgctggagg aacccgtcca aagcgagcgg gcaacgacag aaggaaccga aactgtcgga
     9421 gacgcggtct tcgtcgaaag tcccataaca gaagagactg tagatgatgt tccaatcgcc
     9481 gatgatatta tcgtgctcga tgaacaggag atcgaagccg aagctgcgcc tgccgggctg
     9541 ccggagcaag aggctgaggc aatcgaatta gtagatagtg tagtcgtcga catcgacgac
     9601 acgatagtgg caacagaaga aatcctcgta gaagaattga acgagctaat cgatgaggaa
     9661 tccgctgcga ccctggaaga cgaactgcaa ctgccctcgt ggctggattt gctgaggccg
     9721 ctcgattaaa ctctcccttc gggggaaaca gacaacaaac ttgaattgga gttttctaga
     9781 tggaacaaac gttagtattg ataaagccgg acggcgtgca acgcgggctg gtcggccaga
     9841 ttatcactcg gcttgagcgc cgcggactta agttaatcgg catgaagttc atgcaggttt
     9901 cgactgagtt ggcctcaact cactacgaag cccatgtggg caagccgttc tatgaggggc
     9961 tggtcaatta catcacctcc agccctatcg tcgccatggt gtgggaaggc aaggacgcga
    10021 ttcagatcgt gcgcacgacg atgggcgcga cgaacgctgc cgtggccgcg ccgggcacaa
    10081 ttcgcggcga cttcgctgta gagatcggcc ggaatctcgt acatggctca gacggccttg
    10141 aaagcgccgc ccgcgaagta tcgctattct tcaagtcgga tgaactggtt aactggaaac
    10201 gcgagaccga tcgctggatt cgtgagtaaa gcaggatagc tcactgcata attaaaaagg
    10261 ggcctttcga ggcccctatt gcattctgac gaccacgcgc cctttatgcc ataatgattc
    10321 aaggtcgtaa taggcacgtt tttcctcatc gaagaggtga acgacgacat ccccataatc
    10381 gatcaggatc cagccatcgg ccggagttcc ttcgacaccc ttgggcattc gacccccttt
    10441 ctccttggcc tcgatctgca cagcccgcat caaagcccgt aactggggtt cggactcgcc
    10501 gctacatatg acgaagaaat cggcgaagac agcctgggag tggaggtcca gcacaagaat
    10561 gtcgatgccc tttttatcct gtagagcgtc aacaattaag taagttaatt caaggccttc
    10621 cagacagcac ctccaatact ttaaaataga tgcaggcatt gtaacgtgtt tgcccgccac
    10681 tgaccataga caagttgctc cccttctctc cacgggatag actccggccc attgttctca
    10741 aggataggca ggctgctatg tttccctttc tgaaccaacc cgcccgcgaa gatcgccccg
    10801 cctggagcgt cagtgagctg actacctata tccgcgaaat gttcgagctg gattaccgct
    10861 tgcaagagat cgaggtaatg ggcgagattt ccaacttcac tcgcgccgcc tcggggcatc
    10921 tgtacttcac gctgaaggac ggtgcggccc aaatcaaatg tgtcatgtgg cgctcgcaag
    10981 ccgaacgact gcgcttcagg ccggctgagg gagatgccgt gctggcgcgc ggccgcgtct
    11041 ccgtctacga cgccggtgga gtgtatcagc tctatgccga gcggctgcaa ccggccgggc
    11101 gtggcgatct ggcggttgcc ttcgagaggc tcaaagagga ccttgccggc gaagggctat
    11161 tcgacgccga gttcaagcaa cccatcccgc gcctgccgcg caaaatcggc atcgtcactt
    11221 cggccgatgc ggc
//
LOCUS       DAOMQG010000078        11203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2499_length_11203_cov_6.679046, whole genome shotgun sequence.
ACCESSION   DAOMQG010000078 DAOMQG010000000
VERSION     DAOMQG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2499_length_11203_cov_6.679046"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLC06_06310"
     CDS             complement(<1..280)
                     /locus_tag="PLC06_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90092.1"
                     /translation="MRTIKSLLLIVLLLTAAACGAQPPATPTQAPAEPTAAPAPTEAP
                     VEPTTAPAATDTPVAETPTEAPTEAPTAEPIALPGICDYVDPTSLALNY"
     gene            496..1761
                     /locus_tag="PLC06_06315"
     CDS             496..1761
                     /locus_tag="PLC06_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRO90093.1"
                     /translation="MNSRAYCGHPSFNLIRALGLALLITSVLLPASLSTRYGAARTEA
                     QASGQVHGQAQTHAAYLPMAVGNRAFGLQPVGQGFYVPLGITHAGDERIFIAERGGRI
                     QILHPDGRETTFISLESRVVSSPGEYGLYDVAFHPGYADPDSPGYGFFYVFYTGKEGA
                     DIYSTISRLRVSADPDIADPASETQLFHLQQFNIYHKGGELDFDPRDNTLYAAIGDDT
                     SPPNAQLPDTPKGKIIRLPVDDVPAAAVGDASDLIEPEIVAMGLRNPYRFDLDPQSNR
                     LFIGDVGENTWEEVNVMTLDGTLPNMGWPCREGPEPHTLYQNHPVCAGNKPFVEPVAY
                     LSHADGHCAVIAGKFTRTANRDGEFIFADACSHQIFSMTYSGGAWYSTQLGVSPAGGL
                     LTTIGEDVHGNLYVGLARSNGPIYRLILR"
     gene            1904..2731
                     /locus_tag="PLC06_06320"
     CDS             1904..2731
                     /locus_tag="PLC06_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90094.1"
                     /translation="MRKIWIGAAAWLLLGTVITLAILNSRPATADGADISAGLPVVLK
                     PENTPTPVPSPTPTITPTPTPRVGCPPQPTPTAAAYGLVNGGFEGCWDTLWQQGNQEP
                     HGWELTWIPSGGQLWDARYPEPATAIAEMIHKLKSQLPPDEWPGAPNALILDGGATYK
                     IFSQYNIFGSQLYQRVFLPAGSWRLVVPVQIHWHENLDGKDPYTAESGAWVIANGTQS
                     GKWANAVEMGDRQWYTHEVEFDLHAAATVEVIIRVKSVYRSSKDFFIDAVRIEASDE"
     gene            2918..3346
                     /locus_tag="PLC06_06325"
     CDS             2918..3346
                     /locus_tag="PLC06_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO90095.1"
                     /translation="MATVNQMLMSKSSEVYSIPPDATVLDALRLMAEKDIGALLVMDG
                     DDLSGIFSERDYARKIALQGKSSRTTPIWSVMTNEVICVSPDQTADKCMAIMTDKHIR
                     HLPVIDDGRVVGIISIGDVVKSIMTEQQVFIRHLEDYILS"
     gene            complement(3343..4041)
                     /locus_tag="PLC06_06330"
     CDS             complement(3343..4041)
                     /locus_tag="PLC06_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRO90096.1"
                     /translation="MIELIAFDADDTLWHTERLYREARRQFDELLRPYVDPVSLEAHV
                     HRTEMRNLPYYGFGITSFIMSLIETAVEATHGRVTGAEIGRLLAISREMVAADVELID
                     DVEETLATLAADYPLLIITKGDLLHQTGKVTRSGLGRYFAGVEVVADKTPEVYREILA
                     RRNVAPERFVMVGNSPRSDILPVATLGGRAVYVPDAEPWAYDLVDVPDDLPGTVYRVG
                     RVGEVVGLVRGPGF"
     gene            4134..5225
                     /locus_tag="PLC06_06335"
     CDS             4134..5225
                     /locus_tag="PLC06_06335"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90097.1"
                     /translation="MIQDLYEDLREFAEYSAGHAADKYLSLYLLTDPSHPENQSQTPA
                     WHIYLKNAISDIENSLDPVQTKQWKNVRLSDEDPNKDWARIRKRLDKYITSYRPEGKS
                     LALFISPGGEHRFELPVALTNTHYFGQPHIQEILWALDEYQQHLVLLFAEDQTRALLV
                     ALGSTGADATVTSDQAWLRRERKAAHSQNIQWRQDELTRRNIRAVAGEVDKFFLKNTD
                     IERIILGGNIEMANAVLGELHEAAREKVIAVLPIPIGSPAHEVAKRISETAIHAEREY
                     ETALVNEIVSSAKARGRGAMGGVAVGRAMERSAVRLLALPYPAEDDFEPLLLDAVRQG
                     ARVEFLHDESAERAREAGGILARLYYAIN"
     gene            5785..6420
                     /locus_tag="PLC06_06340"
     CDS             5785..6420
                     /locus_tag="PLC06_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90098.1"
                     /translation="MNKRIVLMAVMLLSLLVSAASVMAQGAPGRSTSRPEAAPAMQVI
                     EAAPTILKLGARLQGSAQHPNIAGWAEYEVERSGNREFEVDVWGAKAFAGKRVEVYVN
                     GKLVGKMKINRAGRGDFDIETSDGDTVPHMKAGDTVEVRFRGAALAGNPLAVVPVSGA
                     NSIDTDDDMDDVDDVDDDIDDDDIDDMDDDDPDDDHDDIDHDDWDDDDDND"
     gene            6604..7149
                     /locus_tag="PLC06_06345"
     CDS             6604..7149
                     /locus_tag="PLC06_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO90099.1"
                     /translation="MEDELELLRRARAFDKEALALIHDRYYPSIYRYLSFRINDPQTA
                     EDLTSEVFIRFLSAIKDRSAPPNTIRGWLYGAARNVLKEQYRKQKRMTWTELDEEITA
                     TGHTPEQRMEERAGRQELREALADLTQEQQDVLALRFGYGMPIKDVAETVNKSEGSVK
                     MLQVRAIAALTRRLTGKEVGH"
     gene            7149..8270
                     /locus_tag="PLC06_06350"
     CDS             7149..8270
                     /locus_tag="PLC06_06350"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HRO90100.1"
                     /translation="MNDTTETILMEALDLLEQGMTADEIVARYPAQAAELRPFLLTAT
                     TLGNLAHQPPLAAQAQSKRAFLDAAGRAAVEPVRSRPLAGGWLRRLMAPALAVLLVLF
                     LGGAGLVNASGAAVPGDALYQAKRWVEDVRLNLATNPETAAELRERFRRERVSEVERL
                     LDEGRAAAVSLSGRIDALDGDRWTVAGVPVRIVAGTTVDGTPIVGALVQVDGRTADGG
                     VVADRVAVLIANLTPEPASPEESTPTPTSPTPSPRPTDDSAPIAPSPSLPVASPVVGP
                     TRPPATRPAPTATPDDDDDDDDGGDAATPDIDDSDDATPDDDADDDGTPDSDDNGDNG
                     DNGDNGDDATPESDDATPDNDDATPDSDNDGTPESDDGG"
     gene            complement(8418..9179)
                     /locus_tag="PLC06_06355"
     CDS             complement(8418..9179)
                     /locus_tag="PLC06_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO90101.1"
                     /translation="MTLATYDYSHHQPRMIDVLIADDHAIVRRGLRTLIAGEPDMTVA
                     GEATDGYEVVYQTRELNPDVILLDLVMPGQSGLEALSQIKIDNPEARVLVLTSFGDNE
                     RVFSAIRAGAMGYLLKDASPEQLLQAIHDVYNGESHLHPTIALKMLRELDNPTLPAPD
                     RPVADEALTEREVEVLRLVAQGLSNQDIAKDLTISERTVGNHIGSILRKLHLANRTQA
                     ALYALKRGLVDINSATLAIPEVEDGEPEIVEEPEE"
     gene            complement(9176..10438)
                     /locus_tag="PLC06_06360"
     CDS             complement(9176..10438)
                     /locus_tag="PLC06_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRO90102.1"
                     /translation="MSDIQELSESFQQQTILALNQFGQVVTSSLKLDEVLARIMEQVT
                     ALLHSEGVAIIMPEGDDRLRFVAVSGIGAAQLKDMSMPRDTGVVGHVMRTGKPVWLNG
                     QGSNVSGLRIYRQIETVSEFHSQSLLASPLNQDGVTIGVLEAAHSDPDGLTADDLAIL
                     CAAAHWAAIAISNARLHEQAQHLRQEQAALEERANLARELHDAVTQSLYSMTVLAGAW
                     RRQIEAGKLAPQYEQIVELGDLAQDALREVRMLIYELRPTELEEEGLVGAIHRRLETV
                     EQRADIKARLVVIDEARSSSQSAEGSIETLLAFHRLPPAVELELFRIVQEALNNVMKH
                     SGATAVTVRFRLGAEEMVLEIEDNGRGTTTDARSRAGAGFGMQSMRERAAQLGGYFSV
                     DSVPGTGTTIRITDIPYRLVEAEDKVMI"
     gene            complement(10646..>11203)
                     /locus_tag="PLC06_06365"
     CDS             complement(10646..>11203)
                     /locus_tag="PLC06_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90103.1"
                     /translation="LTKENLPIIRGRVGLVFQNPDDQLFSPTVLEDVAFGPLHMGLPE
                     DEVRRRAAVALEQVGMAGAEDRLSHHLSIGQKKRIALATVLTMEPEILVLDEPTAGLD
                     PRGRRQLIALLRDLPRSMLVSTHDMALVAELFPRMVVMDEGRIVADDATDRLLALPDL
                     LEAHGLEAPGWRSDERYEGRRTKDE"
BASE COUNT         2155 a         3615 c         3486 g         1947 t
ORIGIN      
        1 cgtaattcag cgccagcgag gtcggatcga cgtagtcaca aatgccgggc agagcgatcg
       61 gctcggcggt cggcgcttcc gtgggggcct cggtgggcgt ttcggccacg ggggtgtcgg
      121 tggccgccgg agcggtggtc ggttccacgg gcgcctcggt gggcgcgggg gcggccgtcg
      181 gctcggccgg tgcctgggtc ggcgtcgccg gcggctgcgc cccacaggcg gcagccgtca
      241 gcaacagcac aatcaatagc aacgatttga tcgttctcat cttccttctc cctaaagtta
      301 aaacaggata gacatggggg cgcgccatgg aatgacgcgg acagctcccc gaaagtcggc
      361 cgacgcatga taccatcggc cgcgcgcgca ttataagaga aaatgcccaa acgtgtaaag
      421 caagatgatt gcaaacactc atatttcttg accatcgccc gatgagaaat gataatgtcc
      481 gcaggaacac cgaccatgaa ctctcgcgcg tattgcggcc acccgtcctt caatctgatc
      541 cgggcgctgg gattggcgct gttgatcacg tctgtcctgt tgcctgcctc gttgtcaacc
      601 cgctatggcg cagcgcgaac tgaagctcag gcctctgggc aggtccatgg acaagcgcag
      661 acgcatgctg cctatctgcc catggccgtc ggcaaccgcg cctttggcct gcagccggtt
      721 ggccaggggt tctacgtccc gctgggaatc acccacgcgg gcgacgagcg aattttcatc
      781 gccgagcgcg gcggccgcat tcagattctg caccccgatg gccgcgagac gacgttcatc
      841 agcctggagt cgcgggtggt ttcctcgccg ggggagtatg ggctgtatga tgtcgctttc
      901 catcccggct atgccgatcc cgattcgccg ggctatggct ttttctatgt gttctatacc
      961 ggcaaggaag gcgccgatat ctactccacc atctcgcggc tccgcgtgtc ggccgatccc
     1021 gatatcgccg accccgcgag cgagacgcag ctatttcacc ttcagcaatt caacatttac
     1081 cacaagggcg gcgaactgga ttttgatccc cgcgataaca ctctctacgc cgcgataggc
     1141 gacgacacca gcccgccgaa cgcccagctg ccggacacgc ccaagggcaa gatcatccgg
     1201 ctgccggtcg atgacgttcc ggcggcggcc gtgggcgatg ctagcgacct gatcgagcct
     1261 gagattgtgg cgatgggcct gcgcaatcct tatcgcttcg accttgaccc ccaatcaaat
     1321 cggctgttca tcggcgatgt gggcgagaac acatgggaag aggtcaacgt catgacgctg
     1381 gacggcacgt tgcccaatat gggttggccc tgccgcgagg ggccggagcc acacaccttg
     1441 tatcaaaacc atcccgtgtg tgccggcaac aaacctttcg tcgagccggt cgcctatctg
     1501 tcccatgccg atggccattg cgccgtcatc gccggcaaat tcacgcgcac agcaaacaga
     1561 gacggcgagt tcatcttcgc cgacgcatgc tcgcatcaaa tcttctcgat gacctacagc
     1621 ggcggcgcgt ggtacagcac gcagttgggg gtttctcccg cgggcggtct gctgacgaca
     1681 atcggcgagg acgtccacgg caacctctac gtcggcctgg cgcgatcaaa cgggccgatt
     1741 tatcggttga tcctgcggta atagtgacga gtggcgaatg acgagtgacg agaattacga
     1801 agtacgaagt acgaaagaag aacacgcctc ctgccgtcgt cggcctatag gcgaatgaga
     1861 gcgcgcctct tttcgtattt cgtacttcga cggaggtaga acaatgcgca agatatggat
     1921 cggtgcggcc gcctggcttt tgctgggcac ggtcataacg ctcgccatcc tgaacagccg
     1981 cccggccacg gccgacggcg ccgacatctc ggccggcctg ccggtcgtcc ttaagccgga
     2041 gaacaccccc acgccggtac cgtcgcccac gccaaccatc actcccaccc ccacgcccag
     2101 ggtcggctgc ccgccgcaac ccacgccgac ggccgccgcc tatggcctgg tcaacggcgg
     2161 attcgagggc tgttgggaca ccctttggca acagggcaac caggagccgc acggctggga
     2221 actgacctgg attccgtccg gcggccaact gtgggatgct cgctaccccg aaccggccac
     2281 ggccatcgcc gagatgatcc acaagctgaa gagccagctc ccgcccgacg aatggccggg
     2341 cgcgccgaat gcgctgatcc tcgacggcgg cgcgacctac aagatcttca gtcaatacaa
     2401 catctttggc agccagctct atcagcgagt gttcctgccg gccgggtcat ggcggctggt
     2461 ggtgccggtg cagatccatt ggcacgagaa cctcgacggc aaagacccct acacggccga
     2521 atcgggcgcg tgggtcatcg ccaatggcac gcaatcgggc aagtgggcca acgccgtcga
     2581 gatgggtgac cggcaatggt atacccacga ggttgagttc gatctgcacg cggccgccac
     2641 ggtggaggtg atcatccgcg tcaagagcgt ctatcgcagc tcgaaggatt tcttcatcga
     2701 tgcggtgagg attgaagcga gtgacgaatg acgaatgacg agtgacgagt gacgagcgcg
     2761 cctttcgtac ttcatcttca ttcgtcactc gtcgctcgca actcgtcacc tgtatttact
     2821 ttccccccgc gatcttttac aatggggcct acatcatcgc tcgcaacccc gcccaaaacc
     2881 acataatgtc gacgcgatcc accaatcgga ggtacttatg gcgacagtta accagatgct
     2941 gatgagcaag agcagcgagg tctactccat tccgcccgac gcgaccgtgc tcgacgcgct
     3001 gcgactcatg gccgagaagg acatcggcgc gctgctggtg atggacggcg atgatctgag
     3061 cggcattttc tcggagcggg actacgcgcg caagatcgcc ctgcagggca agtcgtcgcg
     3121 cacgacgccc atctggtcgg tgatgaccaa tgaggtgatc tgcgtctcgc ccgatcagac
     3181 ggccgacaag tgcatggcca tcatgaccga caagcacatc cgccatctgc ccgtgatcga
     3241 cgacggccgc gtggtgggca tcatctccat cggcgacgtg gtaaaatcga tcatgaccga
     3301 gcagcaggtg ttcatccgtc acctggagga ttacatcctg agctagaagc ccggcccgcg
     3361 caccagcccg accacttccc ccacccggcc cacacgatag accgtgcccg gcaggtcgtc
     3421 gggcacgtcc accagatcgt aggcccacgg ctcggcgtcg ggcacataga cggcgcggcc
     3481 gcccagcgtg gccaccggca ggatgtcgct gcgcgggctg ttgccgacca tgacgaagcg
     3541 ctccggggcc acattgcgcc gggccagaat ctcgcggtag acctccggcg tcttatcggc
     3601 caccacctcg accccggcga aatagcgccc caggccggag cgagtcacct tgcccgtctg
     3661 gtgcagcagg tcgcccttgg tgatgatcag cagcgggtag tcggccgcca gcgtggccag
     3721 cgtctcctcc acgtcgtcga tcaactccac atcggccgcc accatctcgc gggaaatagc
     3781 cagcaggcgg ccgatctccg cgccggtgac gcggccgtgg gtggcctcga ccgccgtctc
     3841 gatgagcgac atgatgaagc tggtgatacc gaagccgtag tagggcagat tgcgcatctc
     3901 cgtccggtgg acgtgggcct ccagagagac ggggtccaca taggggcgca acagctcgtc
     3961 gaattgccgc cgcgcctcgc gatagaggcg ctcggtgtgc cagagggtgt cgtcggcgtc
     4021 gaaagcgatc agttcaatca tgggccgatt ctaccatttg gccggctttt ctggtatcct
     4081 ctgcctgcaa cacggttatt gaacgaccat acctatcagg agctatactc aacatgattc
     4141 aggacctcta cgaagatctg cgtgaatttg ccgaatattc ggccgggcac gccgccgaca
     4201 aatacctctc cctctacctg ctgaccgacc cgtcccaccc ggagaaccag agccagaccc
     4261 cggcatggca tatctatctg aagaacgcca tcagcgacat cgagaacagt ctcgacccgg
     4321 tgcagaccaa gcaatggaag aacgtgcgcc tcagcgacga agaccccaac aaggattggg
     4381 cgcgcatccg caaacgcctg gacaagtaca tcaccagcta ccggcccgaa ggcaaatcgc
     4441 tggccttgtt catcagcccc ggcggcgagc atcgcttcga gctgcccgtc gcgttgacca
     4501 acacccacta cttcggccaa ccccacatcc aggaaatcct gtgggcgctg gatgaatatc
     4561 agcaacatct ggtgctgctc ttcgccgagg atcagacgcg ggcgctgctg gtggctctcg
     4621 gcagcacggg cgccgacgcc accgtgacca gcgaccaggc ctggctgcgc cgcgagcgca
     4681 aggccgccca cagccagaac atccagtggc gacaggacga gctgacccgg cgcaacattc
     4741 gcgccgtcgc cggcgaggtg gacaagttct tcctgaagaa caccgacatc gagcgcatca
     4801 tcctgggcgg caacatcgag atggccaacg ccgtgctggg cgagctgcac gaggcggcgc
     4861 gcgagaaggt gatcgccgtg ctgcccatcc ccatcggcag cccggcccac gaagtggcca
     4921 agcgcatcag cgagacggcc atacacgcgg agcgggaata tgagaccgcg ctggtgaacg
     4981 agatcgtctc ttcggccaag gcgcgcggcc gcggggcgat gggcggggtg gctgtcggcc
     5041 gcgccatgga gcggtcggcc gtgcgcctgc tggccctgcc ctacccggcc gaggacgatt
     5101 tcgagccgct gctgctcgac gccgtgcggc agggcgcccg cgtcgagttt ctccacgacg
     5161 aatcggccga gcgcgcccgc gaggccggcg gaatcctggc ccgcctctat tacgcgatca
     5221 actagcgccg ccattcacgg gaacgggcgg gccaaccgcc gcgcttcccg aacatttgcc
     5281 ggcctgaaag aagccttccg ccaacgcggg aggctttttt tattggcaat cgccccggcg
     5341 aaacccaatt ttccaccaat tcccaatctc cccgttactt tcccgcgcca atcgccactt
     5401 catggtcaga aagcaacaaa atgcaagcga aatcaatcgt ttgctgttga ggggttcttg
     5461 gtttgtaggt tcttggtttg tagtcagccg ctttagcggc gttccccgga gcggcgctaa
     5521 agcgcttact acgagcgaag agcggcgctg aagcgcttac tacgagcgaa gagcggcgct
     5581 aaggcgctta ctacgagcga agagtggcgc taaagcgcct actacgagcg aagagcggcg
     5641 ctgaagcgct tactacgagc gaagagcggc gctaaggcgc ttactacgag cgaagagcgg
     5701 cgctaaagcg cttactacga gcgaagagtg gcgctaaagc gcctactacg aacggtaaca
     5761 aatttttaaa acaggagtct gaaaatgaac aagcgaatcg tgttaatggc agtgatgtta
     5821 ctaagtttgc tggtgtcggc ggcctcggtg atggcccagg gcgcgcccgg cagaagcacg
     5881 agccggccgg aagcggcccc ggccatgcag gtgatcgaag cggccccgac gatcctcaag
     5941 ctcggcgccc ggctgcaggg cagcgcccag caccccaaca tcgcgggctg ggccgagtat
     6001 gaggtcgagc gcagcggcaa ccgcgaattt gaggtggacg tatggggcgc gaaggcgttc
     6061 gcgggcaagc gggtcgaggt ctacgtcaac ggcaagctgg tcggcaagat gaagatcaac
     6121 cgcgccggcc gcggcgactt tgacatcgag acttcggacg gagataccgt cccccatatg
     6181 aaggcaggcg acacggtgga ggtgcgcttc cgcggggccg ccctggccgg caacccgctc
     6241 gccgtagtgc cagtcagcgg cgctaacagc atcgacaccg acgacgacat ggatgatgtc
     6301 gacgatgtgg acgatgacat cgatgacgac gatatcgacg acatggacga cgacgatccc
     6361 gatgatgacc acgatgacat cgaccacgat gattgggacg acgacgacga caatgattaa
     6421 ccgcaggaca gcgtagtact ggaagtccgt caatagaaac cgggtttctt ccgggaaacc
     6481 cggtttctgc acgaccggtg acgaacggcc gcaacaaacg tgcttttctt cgctcgtcac
     6541 tcgtcactct cttgtcgtcc gtcgtcggtc gtcggtcctc attcaatcgg gtatcatcgc
     6601 gtcgtggaag acgaactgga gctactcagg cgagcgcggg ccttcgataa ggaggcgctg
     6661 gctctcatcc atgaccggta ttacccgtcg atctatcgct acctgtcttt ccggatcaac
     6721 gatccgcaga cggccgaaga tttgacaagc gaggtgttca tccggttcct gagcgccatc
     6781 aaggatcgga gcgcgccgcc caacaccatc cgcggctggc tctatggcgc ggcccgcaac
     6841 gtcctgaagg agcaatatcg caagcagaag cggatgacct ggacagagct ggacgaggag
     6901 atcaccgcca ccggccacac gccggagcag cggatggaag agcgagcggg acggcaggag
     6961 ctgcgcgagg cgctggcgga tctgacgcag gagcaacagg atgtattggc tttacgattc
     7021 gggtatggaa tgcccatcaa ggatgtagcg gagacggtga acaagagcga agggtcggtc
     7081 aagatgcttc aggttcgggc aatcgccgcg ttgaccaggc gtctgaccgg gaaagaggtt
     7141 ggtcactgat gaacgacacc acagaaacaa tcctcatgga agcgctcgac ctgttggagc
     7201 agggcatgac ggccgacgag atcgtcgccc gctatccggc ccaggcggcc gaactgcgcc
     7261 cgttcctgct gaccgcgacg acgctgggca acctggccca ccagcccccg ctggccgcac
     7321 aggcgcaatc gaagcgcgcc ttcctcgacg cggccggccg cgccgccgtg gagcctgtcc
     7381 ggtcgcggcc attggccggc ggctggctgc gccgcctgat ggcccccgcg ctggccgttt
     7441 tgctggttct gttcctgggc ggcgcgggat tggtgaacgc atccggcgcg gccgtgcccg
     7501 gcgacgccct gtatcaggcc aagcgctggg tggaagatgt ccgcctcaat ctggcgacca
     7561 acccggagac ggccgccgaa ctgcgcgagc ggttccgccg ggagcgcgtc agcgaggtcg
     7621 agcggctgct ggacgagggc cgggcggcgg cggtgtcgct ctccggccgg atcgacgccc
     7681 tggacggcga ccgctggacg gtggcggggg tgcctgtccg catcgtcgcc gggaccaccg
     7741 tcgacggcac gccaatcgtg ggcgcgctgg tgcaagtcga cggccgaacg gccgacggcg
     7801 gcgttgtggc cgaccgggtc gccgtcctga tcgccaatct gacgccggaa cctgcgtccc
     7861 cggaagagag cacgccgaca cccacgtcgc caaccccttc gccacgcccg accgacgaca
     7921 gcgcgccaat cgccccctcg ccgagcctgc ccgtcgcctc gccggtcgtc ggccccactc
     7981 ggccgccggc aacccgcccc gcgccgaccg ccacgccgga tgatgacgat gacgatgacg
     8041 atggcggtga cgccgctacg ccggatatcg acgacagcga tgacgccacg ccggatgacg
     8101 atgccgatga cgacggtacg ccggatagcg acgacaacgg cgataacggc gacaacggcg
     8161 acaacggcga cgacgccacg cccgaaagcg acgacgctac acctgacaac gacgacgcca
     8221 cccccgatag cgacaatgac ggtacgcccg aaagcgacga cggcggatga actgtagcgc
     8281 aagctgccgg cttgcctctt cgccctgtgg cgcaagctgc cggcttgctc tacagcccga
     8341 aagcgacgac ggcggatgaa cgagattcat ccgccgtcgt cgcccggcaa ttcagttgta
     8401 actgcgttgg gctgcctcta ctcctcaggc tcttcaacga tttcaggctc tccgtcctca
     8461 acttcgggga tcgcgagcgt ggccgagttg atgtccacca ggccgcgctt cagggcatag
     8521 agggcggcct gcgtccggtt ggccaggtgg agcttgcgca ggatgctgcc gatatggttg
     8581 cccacggtgc gctcgctgat ggtcaggtct ttggcgatat cctgattgct caggccctgg
     8641 gccaccagcc gcagcacctc gacctcgcgc tcggtgagcg cctcgtcggc caccggtcgg
     8701 tcgggtgccg gcagagtcgg gttatccagc tcgcggagca tcttcagggc gatggtgggg
     8761 tgcaggtggg actcgccatt atagacgtcg tggatcgcct gcagcagctg ctccggcgag
     8821 gcgtccttca gcaaatagcc catcgccccg gcccgaatgg ccgaaaagac gcgctcgttg
     8881 tcgccgaaac tggtcaatac cagcacgcgc gcttcgggat tgtcgatctt gatctgcgac
     8941 aacgcctcaa gtccgctttg ccccggcatc accaggtcga gcaggatcac gtcggggttc
     9001 agctcccgcg tctgatagac cacctcgtaa ccgtcggtgg cctcgcccgc gaccgtcatg
     9061 tccggctccc cggcgataag ggtgcgcaac ccgcgacgca cgatggcgtg gtcgtcggcg
     9121 atcagaacgt cgatcattcg cggctggtgg tggctgtagt cataggttgc cagggtcata
     9181 tcatcacctt gtcttcggct tcgacgagcc gatatgggat atctgttatt cgaatagttg
     9241 tgccggttcc cggcaccgaa tcgaccgaaa aatatccccc caactgcgcg gcacgctcgc
     9301 gcatactctg catcccgaag ccggcgcccg cacgcgaacg ggcgtcggtc gtggttccgc
     9361 gaccgttgtc ctcgatctcc agcaccatct cttcagcccc caaccggaag cgaacggtga
     9421 cggccgtcgc cccgctgtgc ttcatcacgt tgttcagcgc ctcctgcacg atgcggaaca
     9481 attccagctc caccgccggg ggcaggcgat ggaaagcgag cagcgtctcg atgctgccct
     9541 cggccgactg actcgatgag cgagcttcgt cgatgacgac caggcgggct ttgatatcgg
     9601 cgcgctgctc gaccgtctcc agccgccggt ggatcgcccc gaccagccct tcctcctcaa
     9661 gctcggtcgg tcgcaattca tagatcaaca tgcgcacctc gcgcagcgca tcctgcgcca
     9721 gatcgcccag ttcgacgatc tgctcgtact gcggcgcgag tttcccggcc tcgatctgtc
     9781 gccgccaggc cccggccaaa accgtcatcg aatagagcga ctgggtcacg gcgtcgtgga
     9841 gttcgcgggc cagattggcg cgctcttcga gagcggcttg ctcctggcgc aggtgctgcg
     9901 cctgttcgtg gagccgggcg ttggaaatgg cgatggccgc ccaatgggcg gcggcgcaca
     9961 ggatggccag gtcgtcggcg gtcaggccgt cggggtcgct gtgggcggcc tccagcacgc
    10021 cgatggtcac gccgtcctgg ttcagggggc tggccagcag cgattggctg tggaactcgc
    10081 tgaccgtctc aatctgtcga tagattcgca ggccggagac attactgccc tggccgttga
    10141 gccacaccgg cttgccggtg cgcatgacat ggccgaccac gccggtgtcg cgcggcatgg
    10201 acatatcctt cagctgggcc gcgccgatgc cgctgaccgc gacaaaccgc aatcgatcat
    10261 ccccttcggg catgatgatc gccacgcctt cggagtggag aagcgccgtc acctgctcca
    10321 tgatgcgcgc aagcacttca tcaagcttga ggctggatgt gaccacctgg ccgaattgat
    10381 ttagcgccag gatcgtttgt tgctggaagc tctcggagag ctcctggata tcgctcatta
    10441 ctctactgtc cggtcttccc aagtatccgt attccaatac gaccccggca cggatcaggc
    10501 caaggcaagc caattataca aaaaaaggga gcgtgctgac aatatgacct aggtcatcag
    10561 tcccaatagt gtgatcaaat gttcacaaat tgcttattgc gggggtgagc attgcacaaa
    10621 gtacaaagta tgaaagacgc gcttttcatt cgtccttcgt ccttcgtcct tcgtacctct
    10681 cgtcgctcct ccagcccggt gcttccagcc catgggcttc cagcaggtcg ggcagggcca
    10741 gcaggcggtc ggtggcgtcg tcggccacga tgcggccttc gtccatcacg accatacgcg
    10801 ggaacagctc ggcgacgaga gccatgtcgt gggtggagac gagcatcgac cgcggcagat
    10861 cgcgcagcag ggcgatgagt tggcggcggc cgcgcggatc cagcccggcc gtcggctcgt
    10921 cgagcaccag tatctccggc tccatcgtca ggacggtggc cagggcgatg cgcttcttct
    10981 ggccgatact caggtggtgg gacagccggt cttccgcgcc ggccatgccc acctgctcca
    11041 gcgccacggc cgcccgccgc cgcacctcgt cctccggcag ccccatgtgc agcgggccaa
    11101 aggcgacatc ttccagcact gtgggcgaga agagctggtc gtcaggattc tggaacacca
    11161 gccccacgcg gccgcgaatg atgggcaggt tctctttggt cag
//
LOCUS       DAOMQG010000079        11082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2523_length_11082_cov_8.407001, whole genome shotgun sequence.
ACCESSION   DAOMQG010000079 DAOMQG010000000
VERSION     DAOMQG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_11082_cov_8.407001"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..3243
                     /locus_tag="PLC06_06370"
     CDS             154..3243
                     /locus_tag="PLC06_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO90104.1"
                     /translation="MEENQNNSGKRLSTPILVGFVVVAALILVGLFLPPISLGTRLGL
                     GGGQPAAGVTPEATAGVVESAALPEGVTLTTANGAAVGVSRMAAADLATAADGALAAA
                     AAALPADAVVGDAYVLNFTGDAPTGQIALPLPADLAGAEDVDLRGWDGSAWAYMPSRI
                     DTAAGEVVAAEGALPLAVALVRSNANTQPVISVELAPDAGVPEDVTPLVGETSVGPLV
                     LDQSGSLTGDIAGLPEGNNRFIRITNRGAVVDQAAITAFLNEPAMQDAQIGGLVEQAK
                     AGGFAGVHVDYQGIPAAMSAAFTEYVNRLADALRAEGMQLAITLGTPAPVGNGWDTGG
                     QNWAALGRAADIVHVQMPLDPTAYVENGPAEQLVAWAVQQIDRNKVNLLLTAGAVNKV
                     GDLYTELPSEQALANLGKLALTGGAVEVEPGAAVDVSLEGTASPLEWDGTALAYKYTF
                     EQAGQEQTVWFVSEAALVDRLKLASEFNLRGATVRGLALGSGPAYAAALNNYLGQGDA
                     PQPGGAAIAWTVRDAGDSIIASETGSDLGYTWTAAENPGTYTVEADLVLGETTIDLGE
                     VEVVVGGTVAEAPTATPEATTAPTAPSGGTAGQPAPTAPTANLGPAGEATAVVNSPAN
                     VRTGPSISYGTIAGGAGAGTVVKLIGRNDLSSWYNITLPSGEKGWILGQLVTINAGFN
                     VAGLPVVEAPPPTTATGGTPASGAAAAPIVAPPPVASVGNVGTFELGGQAAGMPSGTM
                     QYAGMKWVKKQHKWNPGDSPSAVAGLINDAHAAGMKILLSIPGQLYPSSIDFEAYTNF
                     LGGVAALGPDGIEVWNEMNIEREWPLGQIDPTSYVNNMLRPAYQKIKAANPNVMVITG
                     APAPTGFFGGCSGAGCDDAPYVAGMMAAGAGSVSDCVGVHYNEGLMPPSATSGDPRGS
                     GGHYTRYFQGMINAYAGAGAGALCFTELGYLSGEEWGSLPAGFLWRPPYNLTVAEHAQ
                     YLAEAVRVGVQSGRVRMIIVFNVDFKTFGDDPQAGYAMIRPNGSCPACETLRQVMGQ"
     gene            3407..4753
                     /locus_tag="PLC06_06375"
     CDS             3407..4753
                     /locus_tag="PLC06_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRO90105.1"
                     /translation="MQRHPKAIIWTITVLMTLILVACGGGEATPEPTAPLENQQPAAT
                     EAVEAVMATLPPPVISGEILPTPTLAPVEGEVVEEAAPTVAPEVSGPFPADQFGYGIQ
                     IHGNATVGNPADTMTAVHDQLGMNWVKAQIQWWLVRPDPDPAVEQWFFYDAVIDEAHN
                     KGLRLMVSVVGAPEWTRAAGGENGPPDDYNEYARFLGELIDRHPGKIDAIEVWNEQNL
                     DREWTSANGISPEDYVRFLQTAYEAIKAKDPNIIVISGALSPTGTGDWVKWADDFEYL
                     DRALAAGMLNYADCVGAHHNGYNIGPDVAYDQAGASPKAATATFRGPFDNPHHSWSFY
                     TTLNTYADKVQAVDPNKKLCVTEFGWASSEGYTEYPVGFEFALDNTLQDQATYITQAF
                     NQMNESNDVWLAFLFNFDFGNKGGGPTDDPTPYSIIDTQGMPRPAFDALAAMPKDP"
     gene            4886..6019
                     /locus_tag="PLC06_06380"
     CDS             4886..6019
                     /locus_tag="PLC06_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90106.1"
                     /translation="MRRLFPLLLIAFMLAGCTPAIDKPALMGDEPVVMSFPSPDYGIH
                     AFLWWKPDTALRDLGLINDMGFTWVKQKFPWREIEGIEKGKFDWYRTDYIVDQVEKAG
                     LKLLVRLDRQPFWSEPLDNQWQENGPPGDPADFGDFCGATAARYRGRIGAYQVWNEPN
                     LSREWGLRPPDPVAYTELLKACYTAIKAADPEAIVISAGLAPTGTDSPEAMPDDKFLQ
                     GMYAAGAADYFDVLGLNAPGYKAPPELDPAEAEANEEYGGGRWFAFRHVEDMRALMVA
                     NGDGHKQVAILEMGWTLDEVNPSYAWHAVDEPTQAAYLVRAYQYAAEHWRPWMGVMVT
                     IYIADWEWTPDDEQWWWSIVLPDGTPRMAYDALKEMEKRKFAE"
     gene            6116..6613
                     /locus_tag="PLC06_06385"
     CDS             6116..6613
                     /locus_tag="PLC06_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90107.1"
                     /translation="MTFWLLVASYWVHLLATVVWLGGIALMAFIAWPALRQGTLSSNQ
                     WFQLQKRFLPWVNAALVVLLITGFVQMTNDENYHGFLAVDSLWAWAMLLKHIAYAGMV
                     ALTAWLQFALYPAMARLALLMETQPEAAMAERDKLAAREIRYLRLNVLTAMLVLLFTA
                     MATAV"
     gene            6761..7408
                     /locus_tag="PLC06_06390"
     CDS             6761..7408
                     /locus_tag="PLC06_06390"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRO90108.1"
                     /translation="MFQAPTNNNVNSIPAVNISRMNRRLKLALDYALTIPTLLLITPL
                     LLVISLLIKLESPGPVLYRRRVVGRNGREFDAYKFRTMYVDSDDKLIADREVWMEVLH
                     GDLDHDPRLTRTGRLLRRYGLDELPRLLNVLNRTMSLVGPRMMTRTELLRFGHRINGY
                     TSVLPGMTGLWQVSGHSRRIDDRIELETRYVREWSMLLDMQILVKSVVVAFTAAA"
     gene            7510..7797
                     /locus_tag="PLC06_06395"
     CDS             7510..7797
                     /locus_tag="PLC06_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90109.1"
                     /translation="MLSIDHTLGIQIARLAAAQRLHGADASYVAVAAALGIPLVSWDK
                     EQVTRAGDVAASAPVLGSNPIYSTATPTDRVNIPFPTQLAQKQTPLASIPA"
     gene            7909..8541
                     /locus_tag="PLC06_06400"
     CDS             7909..8541
                     /locus_tag="PLC06_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRO90110.1"
                     /translation="MDNSIPDIVIGRLPLYLRELTRLEADSDRLTTSSHELAARLGIS
                     SAQIRKDLSHFGEFGKQGTGYHIHYLIERLTEILHLTHEWPVALVGAGFLGHALASYR
                     GFQRRGFVITCVFDDDPTKVGQTVGGLVVQDATDMAAVLRRRDIHIAILAVPAGEAQA
                     TADVLVEAGVRAILSYAPVSLSVPEGVHVSYSDPVVQLQEMAYYLDPGEE"
     gene            8538..9392
                     /locus_tag="PLC06_06405"
     CDS             8538..9392
                     /locus_tag="PLC06_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRO90111.1"
                     /translation="MRGVAETPVVRPVLKWAGGKGRLLGELLGRLPDDFHSRDAAYHE
                     PFIGGGALFFALAGQGRIGQARLSDANPSLIDVYLALRDCVDEVIAVLRGHIHDRDYY
                     YQVRALRPETLSLPERAARVIYLNKTCYNGLYRENRKGEFNVPFGRYKNPTICDEPNL
                     RAASRALQGVDISNRPFTSILDYAQAGDFVYFDPPYHPLSPTANFTAYDRHGFGPDDQ
                     RHLRDVFAALGDRGVRAMLSNSDTPLIRDLYAGFRIDQVFVARAVNSKASGRGKVAEV
                     IVRNFGFD"
     gene            complement(9580..10770)
                     /locus_tag="PLC06_06410"
     CDS             complement(9580..10770)
                     /locus_tag="PLC06_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRO90112.1"
                     /translation="MNNQQLQDLIRHAWANSPAFRNRLDSAGINPNDIQTTADLNRIP
                     VFTKDEAAALQAADPPFGGMLAAPLSAVSRIFFSPGPLYEPGPEEDDEAWGVAVKLLR
                     DAGFSAGEIILNSLSYHLVPAGYLFDGAFTRLGATVIPAGTGPADLQLKMAHDLGATG
                     YVGAPSFLLSLLQKAEEQGITLSIRRAVTTAEPLPPAMRQILTGQYGLEIINTYATAE
                     LGALAVNTGEGMAMRLLPEPIVQVVNPDSGQPVGPGETGEVVVTTSNRLYPLIRFGTG
                     DLAMNIDPRPGESAQEERSIILVGRRGEAVKVRGMFLHPNQLRFASSQVPGVQAMQAI
                     ITRPDGLRDHFVLQVTTAEGTDEAAVAEGLKAAVQGICRVRVDEVGFGEVGDRPVVDE
                     REWN"
     gene            complement(10870..>11082)
                     /locus_tag="PLC06_06415"
     CDS             complement(10870..>11082)
                     /locus_tag="PLC06_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90113.1"
                     /translation="LLVEQNANVALRTADYAYVMENGRIVLDGSAEQLAQNADIKEFY
                     LGLTAVGERKSYRDVKHYKRRKRWLG"
BASE COUNT         2039 a         3554 c         3502 g         1987 t
ORIGIN      
        1 ggttgcccac gcgccgtcat gtgctagaat gcgatctgtt atttaaccgg aatagaaggg
       61 ttgggcggct gcacgatggg ccgctttttt tccatcccaa aagagactga gcattttcag
      121 gtcaagtaat ttccgggaag gggctaaaag ttcatggaag aaaatcaaaa caattcgggc
      181 aaacgcctct ccacgccgat cctcgtcggc ttcgtggttg ttgctgccct tattcttgtg
      241 ggactatttt tgccgccaat ctccctgggc acgcgcctgg ggttgggtgg cgggcagccg
      301 gcggccggcg tcacgcccga ggccaccgcc ggagttgtcg agtcggctgc gctgcctgag
      361 ggcgtgacct tgacgaccgc caatggcgcg gccgtcggcg tcagccgtat ggccgccgcc
      421 gatctggcta cggcggccga tggcgcgctg gcggctgccg ccgcggcgct ccccgccgat
      481 gcggtcgtgg gcgatgccta cgtcctcaac ttcaccggcg acgcgcccac gggccagatc
      541 gccctgccgt taccggcgga cctggccggc gccgaagacg ttgacctgcg cggttgggac
      601 ggcagcgcct gggcctacat gcccagccgg atcgataccg ccgcgggcga agtcgtggcc
      661 gccgagggcg cgttgcccct ggccgtggct ttggttcgct ccaacgcgaa tacgcagccg
      721 gtcatcagcg tcgaactcgc gccggatgcg ggcgtacccg aagacgtgac cccgctggta
      781 ggcgagacat ccgtcggccc gctggttctc gaccagagcg gcagcctcac gggcgacatc
      841 gccggcctgc cggaagggaa caatcgcttc attcgcatca ccaatcgcgg cgcggtcgtc
      901 gatcaggcgg ccatcaccgc gttcctcaac gagcctgcca tgcaagacgc ccagatcggc
      961 ggcctggtgg aacaggccaa ggcgggcgga tttgccggcg ttcacgtcga ctatcagggc
     1021 atccccgccg ccatgtcggc cgctttcacc gagtacgtga atcgtctggc cgacgcactc
     1081 cgcgccgagg gcatgcaact ggccatcacc ctgggtaccc ccgcgcctgt cggcaacggt
     1141 tgggacacgg gcggccagaa ctgggcggcg cttggccgcg cggccgacat cgtccatgtg
     1201 cagatgccgc ttgaccccac cgcctacgtc gaaaatggcc cggccgagca actcgtcgcc
     1261 tgggctgttc agcagatcga ccgcaacaag gtcaacctgc tgctgacggc cggcgcggtc
     1321 aacaaggtcg gcgatctcta taccgaacta ccgtcggagc aggctctggc caatctgggc
     1381 aagctggcgc tgaccggcgg cgcggtggaa gtcgaacccg gcgcggcggt agacgtgtcc
     1441 ctcgagggca cggcttcccc gctggagtgg gacggcacgg cgctggccta taaatacacg
     1501 tttgaacagg ccgggcagga gcaaacggtc tggttcgtca gcgaggcagc gctcgtcgat
     1561 cgcctgaagc tggcctccga atttaacctg cgcggcgcga cggtgcgcgg cctggcgctg
     1621 ggcagcggtc cggcctatgc cgcggcgctg aacaactatc tgggccaggg cgacgcgccc
     1681 cagcccggcg gcgcggccat cgcctggacc gtgcgcgatg ccggcgacag catcatcgcc
     1741 agcgagacgg gcagcgatct gggttacacc tggaccgcgg ccgagaaccc cggcacctac
     1801 acggtagagg ccgatcttgt gctgggcgag accacgatcg atctgggcga ggttgaggtc
     1861 gtcgtcggcg gaacagtcgc cgaggcgccg acagccactc ccgaagcgac taccgcaccg
     1921 accgcgccat ccggcggcac agccggtcag ccggccccga ccgcgcccac ggctaacctg
     1981 ggtccggcgg gcgaggccac ggccgtggtc aactccccgg ccaacgtgcg caccggcccc
     2041 agcatctcct acgggacgat cgcgggcggc gcgggcgcgg gcacagtcgt caaattgatc
     2101 ggtcgcaatg acctgagcag ctggtataac atcaccctgc ccagcggcga aaagggctgg
     2161 attctgggcc agctggtgac aatcaacgca ggcttcaacg tcgccggtct gccggtcgtg
     2221 gaagcgccac cgccaaccac cgccactggc ggtactccgg ccagcggcgc cgctgctgcg
     2281 ccgatcgtcg ccccgccgcc cgtggcctcc gtcggcaacg tgggcacctt cgagctgggc
     2341 ggccaggccg ccggcatgcc ttccggcacc atgcagtacg cgggcatgaa atgggtgaag
     2401 aagcagcaca aatggaaccc cggcgactcg ccttcggccg ttgccggtct catcaatgac
     2461 gcccacgccg cgggcatgaa gatcctgctc agcatccccg gtcagctcta cccgagcagc
     2521 atcgacttcg aagcctacac caacttcctg ggcggcgtgg ccgcattggg gccggacggc
     2581 atcgaagtct ggaacgagat gaacatcgag cgcgaatggc cgctgggcca gatcgacccg
     2641 acatcctacg tcaacaacat gctgcggccg gcctaccaga agattaaggc cgccaacccc
     2701 aacgttatgg tcatcaccgg cgcgcccgcg cccaccggtt tcttcggtgg ttgcagcggc
     2761 gcgggctgcg atgatgcgcc ctacgtggcg ggcatgatgg ccgccggcgc gggcagcgtg
     2821 tcggactgcg tgggcgtcca ctacaatgaa ggcctgatgc cgccgtcggc caccagcggc
     2881 gacccgcgcg gttcgggcgg ccattacacc cgctacttcc agggcatgat caacgcctat
     2941 gcgggagcgg gagcaggagc gctctgcttc accgaactgg gttatctgag cggtgaagag
     3001 tggggttcgc tgccggccgg cttcctgtgg cggccgccgt ataacctgac ggtggccgaa
     3061 cacgcgcagt atctggctga ggcggttcgc gtcggtgtcc agagcggccg cgtgcgcatg
     3121 atcatcgtct tcaacgtcga cttcaagacg tttggcgacg acccgcaagc cggctacgcc
     3181 atgatccgcc ccaacggcag ctgcccggcg tgcgaaacgt tgcggcaggt gatgggacaa
     3241 taaatgtagc tcaacctgtc gaggttgagg gttaaagaag acacaagccg ggcgggttgg
     3301 gctgcaatta caatgcggcc caacctgtcc ggcatgaaat ccaggaagat gcaagccgga
     3361 cggcttgcgc tacatacagg gttcaataaa gcgaggagag attcccatgc agcgacaccc
     3421 gaaagcgatc atctggacga tcaccgtatt gatgacgttg atcctggtgg cttgcggcgg
     3481 cggagaagcc acccccgagc cgaccgcccc actggagaac cagcagcccg ccgccaccga
     3541 ggcggtcgaa gccgtcatgg ccacgctacc gccgccggtc atctccggcg aaatcctgcc
     3601 cacgcccacg ctggctcccg tcgagggcga ggtggttgaa gaggctgccc cgaccgtggc
     3661 accggaagtc agcgggccct tcccggccga ccagttcggc tacggcatcc agatccatgg
     3721 caacgccacc gtcggcaatc cggccgacac gatgacggcc gtacacgatc agttgggcat
     3781 gaattgggtg aaggcccaga ttcaatggtg gctggtgcgg ccggacccgg atccggcggt
     3841 cgagcagtgg ttcttctatg atgccgtcat cgacgaggcc cacaacaagg ggctgcgcct
     3901 gatggtcagc gtcgtgggcg cgccggaatg gacgcgtgcc gcgggcggcg aaaacggccc
     3961 gcccgacgac tacaacgagt acgcccgctt cctgggtgaa ctcattgacc gccatccggg
     4021 caagatcgac gctatcgagg tctggaacga acagaacctc gaccgtgaat ggacgagcgc
     4081 caacggcatc agccccgaag attatgtgcg cttcctgcaa accgcctatg aggccatcaa
     4141 ggccaaggac cccaacatca tcgtcatcag tggcgcgctc tcgccgacgg gcacgggcga
     4201 ctgggtgaag tgggccgacg atttcgagta tctcgatcgc gctctggcgg cggggatgct
     4261 caactatgcc gactgcgtgg gcgcgcatca caatggctat aacatcggcc ccgatgtggc
     4321 ctacgatcag gccggcgcat cgcccaaggc ggccacggct accttccgtg gcccgttcga
     4381 caacccccac cactcgtgga gcttctacac gacgctcaac acctacgccg acaaagtgca
     4441 ggccgttgat cccaacaaga agttgtgtgt aaccgaattc ggctgggcca gcagcgaggg
     4501 ctacaccgag tatccggtcg gcttcgagtt tgcgctcgat aacaccctgc aagaccaggc
     4561 gacctacatc acccaggcgt tcaatcagat gaacgaatcg aacgacgtct ggctggcctt
     4621 cctgttcaac ttcgacttcg gcaacaaggg cggcggcccc accgacgacc caacgcctta
     4681 cagcattatc gacacccagg gcatgcctcg cccggccttc gatgcgttgg cagcgatgcc
     4741 gaaggacccg taggcagtgg atcagtaggt cagtaggtca gtaagatcag taggtcagtg
     4801 gatcagtcta ctgatctact gaacattgac ctactgaata ctgatttact gaacaccgat
     4861 ctactgaata ccgcccgctc ctcccatgcg tcgcctcttc ccccttctct taatcgcctt
     4921 catgctggcc ggctgcacgc cggcgatcga caagccggcg ttgatgggtg acgagccggt
     4981 agtgatgtcc tttccctcgc ccgattacgg catccacgcc ttcctgtggt ggaagcccga
     5041 cacggcgctg cgcgacctgg gcctcatcaa cgacatgggc ttcacctggg tcaagcagaa
     5101 attcccgtgg cgcgagattg agggcattga gaaggggaag tttgactggt atcgcaccga
     5161 ctacatcgtc gatcaggtgg agaaggcggg gctgaagctg ctggtgcggc tcgatcgcca
     5221 gccattctgg tcggagccac tcgataatca gtggcaggag aacggccctc ccggcgaccc
     5281 ggccgacttc ggcgatttct gcggcgcgac ggccgcccgc tatcgaggcc gtattggggc
     5341 ttatcaggtg tggaacgagc cgaacctgtc gcgcgagtgg ggcttgcggc cgcccgatcc
     5401 ggtggcttac accgaactgt tgaaagcgtg ctacacggcc atcaaggcgg ccgaccccga
     5461 ggccatcgtc atctccgccg gactggcccc caccggcacc gacagccccg aggccatgcc
     5521 cgacgacaag ttcctgcagg gcatgtacgc cgccggcgcg gccgactatt tcgacgtgct
     5581 gggcctgaac gcgccgggtt acaaggctcc gccggagctg gacccggccg aggccgaggc
     5641 gaatgaggaa tacggcggcg gccgctggtt cgccttccgc catgtcgagg acatgcgcgc
     5701 gctgatggtg gctaacggcg acggtcacaa gcaggtcgct atcctggaga tgggctggac
     5761 gctcgacgaa gtcaatcctt cctacgcctg gcacgccgtg gacgagccga cgcaggccgc
     5821 ctacctggtg cgcgcctatc agtatgcggc cgaacattgg cggccgtgga tgggcgtgat
     5881 ggtgaccatc tacatcgccg attgggaatg gacgcccgac gacgagcagt ggtggtggtc
     5941 gatcgtgctg cccgacggca cgccgcgtat ggcgtatgat gcgttgaaag agatggagaa
     6001 gcggaaattt gcagaatgag caggggggaa agggagaaag ggagaaaggg tgatggggag
     6061 acctcttcac cctttctccc ctgcccccct gctcccccgc tcgctgaggt aagcaatgac
     6121 attctggctg cttgtggctt catactgggt tcatctgctg gcgacggtcg tctggctggg
     6181 cggtattgcg ttgatggctt tcatcgcctg gccggccttg cggcaaggca cgctgtcgtc
     6241 gaatcaatgg ttccagttgc agaagcgctt cctgccctgg gtcaacgcgg cgctggtggt
     6301 gctgctcatc accggcttcg ttcagatgac caacgacgag aactaccacg gctttctggc
     6361 cgtcgatagc ctctgggcgt gggccatgct gctgaagcac atcgcctacg caggcatggt
     6421 cgccctgacc gcctggctcc agttcgcgct ctacccggcg atggcccggc tggcgctgct
     6481 gatggaaact cagccggaag cggccatggc cgaacgcgac aagctggccg cccgcgagat
     6541 tcgctatctg cggctcaatg tgctgacggc gatgctggta ttgctgttca cggcgatggc
     6601 gacggccgtg tagcgcagac attcctgtct gcgctccaag aattttccgt aaaaactccg
     6661 ttagttttcc gtaaggaatt tcgtaagaaa gccaatctac actatcccca ggcgattagg
     6721 atgtctcgcg gctgcgcggg cgccaaggcg agggataggc atgtttcagg caccgacaaa
     6781 caacaacgtc aactcaattc ccgcagtcaa tataagccgg atgaaccgcc ggctcaagct
     6841 cgccctggac tacgccctga ccattcccac cctgttgctc atcaccccgc tgctgctggt
     6901 catctcgctg ctcatcaagc ttgaatcgcc cggtccggtg ctctatcgcc gccgggtagt
     6961 gggccgcaac ggccgcgagt tcgacgccta caagttccgc accatgtacg tcgacagcga
     7021 cgacaagctc atcgccgacc gggaagtgtg gatggaggtg ctccatggcg acctcgacca
     7081 cgacccgcgc ctgacccgca ccggtcgcct gctgcgtcgc tacggtctcg atgagttgcc
     7141 gcgactgctc aacgtgctca accgcacgat gtcactggtc ggaccgcgca tgatgacccg
     7201 caccgagctg ctccggttcg gccaccgcat caacggctac accagtgtgc tgcccggcat
     7261 gaccggactg tggcaggtga gcggccacag ccgtcgcatc gacgaccgga ttgagctgga
     7321 aacccgctac gtgcgcgagt ggtcgatgct gctggatatg cagattctgg tgaagtcggt
     7381 ggtggtggcg tttacggccg cggcctgagg acgcttcgtt taatcggtgg ggccatcgcg
     7441 cggcagacgg gcgacccaac gctaggccgc aaaacgatcg accggttgct ggctattccc
     7501 aacctgcgat tgctcagtat cgatcacact ttgggcattc aaatagcccg gctcgccgca
     7561 gcgcaacgcc tgcacggggc agatgcgtcc tacgtggctg ttgctgccgc tttgggcatc
     7621 cctctggtca gttgggataa agaacaagtg acgcgagcag gagacgtggc ggcgagcgca
     7681 ccagtattag gaagcaaccc catctattca accgctacgc cgaccgacag ggttaatatt
     7741 ccatttccga cacaattggc acaaaagcaa acgccacttg cttccatacc tgcttagacc
     7801 ccatcaacca ttatttaccc atgacgcccg aacaattttg gcctgctgcc gttcatctgt
     7861 tacaatgttc gtgaaacttt gcacgattaa gtaccaggaa cccggcccgt ggacaactca
     7921 atccccgaca tcgtaatcgg ccgtctgcct ctctatctcc gcgaactcac tcgcctggag
     7981 gctgactccg accgcctcac cacctcgtcc cacgaattgg ccgcgcggct gggcatcagc
     8041 tccgcccaaa tccgcaagga cctgtcccac ttcggcgaat tcggcaagca aggcacgggc
     8101 tatcacatcc actatctcat cgagcgactg acggagatat tgcatctgac ccacgaatgg
     8161 ccggtggcgc tggtgggcgc gggctttttg ggtcacgcgc tggccagcta ccgcggcttc
     8221 cagcggcggg gattcgtcat cacctgtgtc ttcgacgacg acccgaccaa ggtcggccag
     8281 acggttggcg gactggtggt gcaggatgcg accgacatgg cggcagtgct gcgccggcgg
     8341 gatatccata tcgccatact ggccgtgccg gccggcgagg cacaggcgac ggccgacgtg
     8401 ctggtagagg ccggggtgcg ggccatcctc agctacgccc ccgtctcgct gagcgtgccc
     8461 gaaggcgtcc acgtcagcta tagcgacccg gtcgtgcagt tgcaggagat ggcgtactat
     8521 ttggatccgg gagaggagtg aggggcgtcg cggaaacacc tgtcgtgcgg ccggtgctga
     8581 agtgggccgg gggcaagggg cgactgctgg gcgagctgct cggccgcctg ccggacgatt
     8641 tccacagccg cgatgcggcc taccacgaac cgttcatcgg cggcggggcg ttgttcttcg
     8701 ctctcgccgg gcaaggtcgc atcggtcagg cacgcctctc cgacgccaat ccgtcgctga
     8761 tcgacgtcta tcttgccctg cgcgattgcg tcgatgaggt gatcgccgtc ctgcgcgggc
     8821 atatccatga ccgcgactac tattatcagg ttcgcgccct gcggccggag acgctgagcc
     8881 tgccggagcg cgcggcgcgt gtcatctacc tcaacaagac ctgctacaac ggcctctatc
     8941 gcgagaaccg gaagggggag ttcaacgtcc ccttcggccg ctacaagaat cccaccattt
     9001 gtgatgagcc gaatctgcgg gcggcgtcgc gggcgctgca aggggtggat atcagcaatc
     9061 gccccttcac ctcgatacta gactatgcgc aagcagggga tttcgtctac ttcgacccgc
     9121 cctatcatcc cctgtcgccc acggccaact tcacggccta tgaccgccac ggcttcggcc
     9181 ccgacgacca gcggcacctg cgcgacgtat tcgccgcgct gggcgaccga ggcgtgcggg
     9241 cgatgctatc caattccgat acgccgctca tccgcgatct gtacgccgga tttcggatcg
     9301 atcaggtgtt cgtggcacgg gcggtgaatt cgaaagccag tgggcggggt aaagtggcgg
     9361 aagtgatcgt gcggaatttt ggctttgatt aagccctgct ttctagcaag aacaagcact
     9421 tgacccgatt atgagtctcg attcccttcg ataagcggcc agttgccgat tgtttttgcg
     9481 aagatgcctt tcagaaaggg tgtctttatc ggacggtgcc ggagagagca cgctgactta
     9541 aaagagtgct gtcatccgga ccgaatggct atatcaacct caattccatt cccgttcatc
     9601 gaccaccggc ctgtctccga cctcaccaaa ccccacctca tccacccgca ctcgacatat
     9661 cccttgcacg gccgccttca gtccttcggc gacggccgct tcgtcagtgc cctcggctgt
     9721 agtcacctgc agcacaaaat gatcgcgcag accgtcgggt cgggtgatga tcgcctgcat
     9781 ggcttgcacg cccggcactt gcgaggaggc aaagcgcagc tggttggggt gcaggaacat
     9841 gccgcgcacc ttcaccgcct cgccgcggcg gccgacgaga atgatcgacc gctcctcctg
     9901 cgcgctctcg ccggggcgcg ggtcgatgtt catcgccagg tcgcccgtgc cgaagcgaat
     9961 gagcgggtag agacgattgg aggtggtgac gacgacctcg cccgtttccc cgggaccgac
    10021 aggttggccg ctgtcaggat taaccacctg cacgatcggc tcgggcagca accgcatggc
    10081 cattccctcg ccggtgttga ccgccagcgc gcccagttcg gcggtggcat aggtgttgat
    10141 gatctccaat ccatattggc cggtgagtat ctgccgcatg gccggcggca gcggctcggc
    10201 cgtggtaacc gcccggcgga tggacagggt gatgccctgc tcttcagcct tttgcagcag
    10261 gctcagcagg aagctgggtg cgcccacata gccggtcgcg cccaggtcgt gggccatctt
    10321 gagttgcagg tcggccgggc cggtgccggc ggggatgacc gtcgcgccga gacgggtgaa
    10381 cgcgccatcg aacagatagc cggccgggac gagatgatag gagaggctgt tgaggatgat
    10441 ctcccccgcg ctgaatccgg catcgcgtaa cagcttcacg gccacgcccc aggcttcgtc
    10501 gtcttcctcc ggccccggct catagagcgg cccgggcgag aagaagatac gactgacggc
    10561 cgacagcggc gcggccagca tgccgccgaa gggcgggtcg gccgcctgca acgccgcggc
    10621 ttcatctttg gtgaacaccg ggatgcggtt cagatcggcg gtcgtttgaa tatcgttggg
    10681 gttgattccc gcgctgtcga gccgattgcg aaatgcgggc gaattggccc aggcgtggcg
    10741 gatgaggtct tgcagttgtt gattgttcat ttgacagtat ccggtaggtc agttgtcagc
    10801 aggtcagcga gcgtaggtca gcgggaccga atactgaata ctggtttaca gacctactga
    10861 acactgacct cagcccagcc accgcttgcg ccgcttgtaa tgcttcacgt cgcggtagct
    10921 cttgcgctcg cccacggccg tcaggccgag gtagaactcc ttgatgtcgg cgttctgggc
    10981 cagttgctcg gccgagccgt cgagcacgat gcggccgttt tccatcacat aggcatagtc
    11041 ggccgttcgc aaggcgacgt tggcgttttg ctccaccagc ag
//
LOCUS       DAOMQG010000080        11043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2530_length_11043_cov_6.909174, whole genome shotgun sequence.
ACCESSION   DAOMQG010000080 DAOMQG010000000
VERSION     DAOMQG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_11043_cov_6.909174"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>207
                     /locus_tag="PLC06_06420"
     CDS             <1..>207
                     /locus_tag="PLC06_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90114.1"
                     /translation="ISVRTGTYAERLHIQKPGTTDSPILIAAFQNEKPVIDGTGLNLA
                     NDSALVIIQQSQDVQLAGFTIRNS"
     assembly_gap    208..233
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <234..2445
                     /locus_tag="PLC06_06425"
     CDS             <234..2445
                     /locus_tag="PLC06_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO90115.1"
                     /translation="GILISRSSRIIIRNVTVESSYAGGLHAKQVDKLLVEGSLIHNCA
                     RRFLAYGPEQMNVALLVRGCQDVVVQNNQVYENSDEGVAIGLGCQRVIVRKNTCYDNR
                     NGQISVTSAVEVDVDSNLCYHTGRADFLTLNGERGPGIIKDDLWRYQTGGKWHTRNVN
                     FINNIVVGCGIGFNAVRRGGKLSNIQIAHNTVLNSSKHAIYLKTRARSTMSFIENNLF
                     ASSSDGEMVYALGGEGIVWRHNLWSSFPGENVYNPSNDVVEAEVGLANPRAPIEPGAV
                     TADSYKLVSTSRAINNGIRNKGEISNDFWGSPRDAKPDIGANEYPAGSADPPAGDEPA
                     LPPLGIRVTKGLASLYEFKEKQGREVHDTSGISDALTLYIKDESRVTWTEDGLLIKEP
                     VLISSERPATKIIKACRQSNEITLEAWIRPANIIQDGPARVISISNSKTQRNATLGQG
                     LHGNRPADLFMARLRTTQTSTNGLPAVVSPPGTASGDLTHIVYTRHIDGRSVLYINGQ
                     DRGILDVDGDFSNWDETMPLLLGDEVSEDRPWLGLLHLCAIYSRALVPTEVLHNYEAN
                     YQSDVNLSADFSILPEDEHGIIPHVVEFDSSDSIAAAGITAYFWEFGDGQTSSRANPV
                     VTYTAPGVYSVSLTITDANGQTDKLTKEGLITVAEKPVAPLPAEYARFILVNIANSKV
                     MAFGIQYPDLRCSLLWNDEPHHMIVYADVEDVERVYTTGNMVELIWVDQLEEEY"
     gene            2511..4496
                     /locus_tag="PLC06_06430"
     CDS             2511..4496
                     /locus_tag="PLC06_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRO90116.1"
                     /translation="MTTYYVDGNSGNDSNNGSSDSPWKRLGKALSKVQPGDEVRVRTA
                     TYNEELTLTVRNTTWKADTGHKPVLDGRYHEGLFRPDRTLPTPVPGSGYLPDKLGNMV
                     TLREEGVILDGFTIQNCSGTGIGVTSSNTIVRNCRVDFTYDSSIKVNATAFIDNVIVE
                     NNVCTRSSMRYFDPGRNQTGGPQSVNGVMKMGRTRDGIIRNNIFAYGHGEGINIGKGN
                     YRILVEGNVVHTCNHVHIYINRSIDTIIRNNLVYHLYTPDYLGPNMRPPAGIIYGDEY
                     AQGRPWENSSGGQVYNNIVVGLGRLFMVRNNENNYDTQLDNAYIGFNTFIGGPLTEIG
                     IQISGNYKGRPHKNSLFENNIITNVSVISQASGDLKGIFFRNNLWDEQPHAALRGPGD
                     RIGNPNLVNPNAEIKDLFPDPGNNVDPRNYQLTSRSSLAIGMASDSSQANSIKPPAVT
                     KDFFGANRDNKPDIGAHEYAGVITALTANFSIGPSQDAGSIPHTVDFIDKSISEGVIM
                     SRLWDFGDSGTSSETNPSHTYTTAGTYDVTLTVTDDKGNTDTMVQSGLITALAEPDTS
                     IPDSFRRFILIQKDNQQVMAYGTQYPDLRCVVIWKVDPFHLMNFNDIADVVRNVHVAD
                     TNEVLWIDPGDLDEPVLTYDDSEMFGQSMPTSTLLRG"
     gene            complement(4565..5422)
                     /locus_tag="PLC06_06435"
     CDS             complement(4565..5422)
                     /locus_tag="PLC06_06435"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRO90117.1"
                     /translation="MQLPNFIIGGVIKGGTTSLNYYLKQHPDVFMSRFKEPRYFAYEP
                     DNPDHVEGHGLRFPIRTLTEYAALFEEATTEQAIGESSPHYLRSPQAPRLIKETIPDV
                     RLIFSLRDPVKRAYSSYWHKVRLGIEDRPVEDALMEQDQAVQHGLYSQSLEKWNALFD
                     PAQIKIILFEDLISDTLGVFANLCRFLEVDDAFVPDLVIRNKGGAMKNRRLGRVYEQI
                     KTHPLRQAIDPYVPAKLRQMMINTRNNNLEEPPPIPLPIVRRLYDFYREDIEKLESMI
                     NRDLSIWKN"
     gene            complement(5503..6459)
                     /locus_tag="PLC06_06440"
     CDS             complement(5503..6459)
                     /locus_tag="PLC06_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90118.1"
                     /translation="MTRKVIFVGGTSYSGSTFFHLTLANDPKGFAVGEVKHLFRPTKE
                     RHARPTWACGCGDPECTLWNRVKKNGEAHLYESIFEIHPEVEFIVDASKNVVWIDDQI
                     NYLSRQGIEVQSVVIWKTLMEFAHSLKKRNRLEAKDGAELANWPRYHRNFYSFVEGFR
                     TVKYADYARDQAAVLQATCACLDIPYFEGKERYWEKVHHSLGGNLSSRIHLYSKESDR
                     FQDVQQRAKGHRSDHVADDRNYRQVYYERPDETTLEAHIARLRQDSPYLDSIEEMLAA
                     HDVTNAVLPDDEWPELKLGMIDMYMKQLRQSARMRIGKLRFV"
     gene            6612..7757
                     /locus_tag="PLC06_06445"
     CDS             6612..7757
                     /locus_tag="PLC06_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRO90119.1"
                     /translation="MQIKPFATELFFNLYEFTTPHLLCASDCETLSVSELLHLAGANP
                     SELLDLRLGYTESPGHPHLRAAVSRLYERTSADEIVILGAPEEGIYLTMRALLEPGDH
                     VIVLTPAYDSLLNLAEHASGNVSRWPIRPTSSGWALDLEELGRLITDDTRLVVVNFPH
                     NPTGYLPAATELGALIDIARRHGAWLLCDEMYRGLERNPADRLPSAADRYERAIVLSG
                     LSKTYGLPGLRTGWLVVGDPATRAAIINWKHYTTICAAAPSETLATIALSAHEALAMR
                     NRRIISGNLKAADAFFSRWPDLFDWRPPRAGSIALVGIDVPSATEYCHALARNAGVLL
                     LPGPCLGADDHSVRFGFGRLGFQESLAAYEQFLESERQDAPARFSKP"
     gene            7729..8298
                     /locus_tag="PLC06_06450"
     CDS             7729..8298
                     /locus_tag="PLC06_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRO90120.1"
                     /translation="MPRLDFQSLDDLAAWLATSGIDVSEWGNDDSKTTADLWHEFVVG
                     ESSFIDDPPGRLVEVAQILIRWGDAILIEIEQEFVDGRRRVRMRPPSEKLVRGEEPRT
                     AAIRCLKEELGLSSAEVTVGADHEITEESVDSPSYPGLPTRYRFYTFEVATDSLPNGD
                     FCRENTASNDPIRRHLWGWRAEWAVNSSQ"
     gene            complement(8387..9298)
                     /gene="pdxS"
                     /locus_tag="PLC06_06455"
     CDS             complement(8387..9298)
                     /gene="pdxS"
                     /locus_tag="PLC06_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRO90121.1"
                     /translation="MKENGNVNGRGEVGSRTVKEGFAQMLKGGVIMDVVTPEHARIAE
                     EAGAVAVMALERVPADIRVSGGVARMSDPGLIKEIMAAVSIPVMAKVRIGHFVEAQIL
                     EALGVDFIDESEVLTPADESYHINKNDFKIPFVCGCRNLGEALRRVGEGAAMIRTKGE
                     AGTGDVVEAVRHARTVLGDIRRLQNMPEEELMTFAKEIGAPYELVKETRELGRMPVVN
                     FAAGGIATPADAALMMQLGVDGVFVGSGIFKSGDPARRAKAIVEATTHFRNAEILARV
                     SENLGQPMVGIGIHTLAEDQQLATRGW"
     gene            complement(9523..9984)
                     /locus_tag="PLC06_06460"
     CDS             complement(9523..9984)
                     /locus_tag="PLC06_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HRO90122.1"
                     /translation="MKLNPLFLITMLLLAACSGGAVANPTAEPTIEPALKPAAELANP
                     ASANCVEQGGILNLESRPDGGQFGVCYFMDNRQCEEWALLRGECPVGGLKITGYITEA
                     GRFCAITGGQYDITQEGDTATERGTCTLPNGEVCEAQAFYEGSCGPGAEVN"
     gene            10262..10936
                     /locus_tag="PLC06_06465"
     CDS             10262..10936
                     /locus_tag="PLC06_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRO90123.1"
                     /translation="MQFDKLDSEFLNYLVTNHVNPGDRLPALSEIGDGMGVSVGKLRE
                     QLAVARGMGVVSVKPRLGIQREPFNFAESVLPAVMFSLATGEARFTHLSRLRQVIEVS
                     FWDEAVVLLTPDDKARLRQIVADAWDKLRGEPIHIPNGEHRRLHMTIFSHLNNPFVQG
                     FLVAYWDAYDASELTRFMRYRYWVEVWSYHERIVDALCADDFECGRQLLIEHFSLLPT
                     EPGAES"
BASE COUNT         2520 a         3186 c         2863 g         2448 t
ORIGIN      
        1 catctccgtc cgcacaggca catacgccga gcgtctgcac attcaaaagc caggcacgac
       61 cgattcacca atcctaatcg ctgcctttca gaacgagaag ccggttatcg atggtactgg
      121 cctcaacctt gccaatgact ccgcgctagt gatcatccag cagagccagg atgtgcagtt
      181 ggcagggttc accatccgca attccagnnn nnnnnnnnnn nnnnnnnnnn nnncggcata
      241 ttgatctcca ggtcgagccg gatcatcatc cgcaacgtga cggttgagtc gtcctatgcc
      301 ggtggcctcc atgccaagca agtggacaag ctcctggtgg aaggatcgct tattcataat
      361 tgcgcgcgtc gttttctggc ctatggcccg gagcaaatga acgtggccct tctggtacgc
      421 ggctgccagg atgttgtagt tcagaataat caggtctatg aaaattctga tgagggagtt
      481 gctatcggcc tgggctgcca gcgagtgatc gtgcgcaaga acacctgcta cgataatcgt
      541 aacggacaga ttagcgtcac ctctgctgta gaggtagatg tcgattcaaa tctttgctac
      601 cataccggtc gagcagattt cctgacgttg aacggagaac gtggcccggg catcattaaa
      661 gatgatctgt ggcgctacca aacgggtggc aaatggcaca cacgcaatgt caacttcatc
      721 aacaacatcg tcgttggctg tggaattggc ttcaacgcag ttcgtagagg tggcaagctg
      781 agcaatatcc agattgccca caacacggtc ttgaacagca gcaagcacgc aatctatcta
      841 aagacacgtg cgcgaagcac aatgtccttc atcgagaaca atctcttcgc ctcaagttcc
      901 gatggtgaaa tggtctatgc cctgggcgga gagggaatcg tctggcgaca caatctatgg
      961 tcatcgttcc caggcgaaaa cgtctataat ccttcaaatg atgtggtaga ggccgaagtc
     1021 ggcctggcta atccgagagc acccattgaa cccggtgcag taacggccga ttcatacaaa
     1081 ctggtaagca cttctcgggc gattaacaac ggaatccgta ataaaggcga gatctcgaat
     1141 gacttctggg gctcgccacg agatgcaaag ccggatattg gcgcaaatga atatccggca
     1201 ggaagcgccg atccgccagc gggagatgaa ccggcattgc caccccttgg catacgcgtg
     1261 acaaaaggcc tggcttccct atatgaattt aaggaaaagc aaggtcgaga ggttcacgat
     1321 accagcggga ttagcgacgc gctcaccctg tacattaaag acgaatcacg tgttacatgg
     1381 actgaggatg ggctattgat caaggagccg gtcctgatct cctctgaacg gccagccacc
     1441 aaaattatca aagcttgtcg tcaatcgaat gagatcacgc tcgaagcatg gatacgtcct
     1501 gccaatatca tccaggacgg ccccgcgcgc gtcatcagta tctctaatag caagacccaa
     1561 cggaacgcca ctctgggcca gggattgcat ggcaatcgtc cagccgacct gttcatggcc
     1621 cgccttcgta ccactcagac ttcgaccaat ggcctgcctg cggtcgtctc cccgcccgga
     1681 acagcatcag gcgacttgac ccatatcgtc tatactcgac acattgacgg gcgatctgtc
     1741 ctgtatatca atggccagga tcgaggcatt ctggatgtcg atggcgattt cagcaattgg
     1801 gatgagacaa tgcccttgct ccttggcgac gaggttagcg aagacaggcc atggttaggc
     1861 ctattacatt tatgcgctat ctactcacgc gcgctggttc cgacagaagt actgcataat
     1921 tacgaagcta attatcagag tgacgttaac ctctcggccg acttctccat cctgcccgag
     1981 gatgaacatg gcatcatccc tcacgtcgta gaatttgatt ccagcgatag cattgcagca
     2041 gcgggcataa ccgcttattt ttgggaattt ggcgacgggc agacttccag ccgtgccaat
     2101 cctgtcgtga cttataccgc gccaggcgtc tattccgtct cgctaaccat caccgacgcc
     2161 aatggccaga ctgacaagct caccaaggaa ggcttgatca cggtagccga aaagccggtt
     2221 gcgccgctgc cggccgaata tgctcgcttc attctggtca atatcgccaa ttccaaagtt
     2281 atggcgtttg gcatccagta ccccgacttg cgttgctccc ttttgtggaa tgacgaaccg
     2341 caccatatga tcgtctacgc cgacgttgaa gatgtagagc gagtctacac gaccggcaat
     2401 atggtggaat tgatttgggt cgatcaattg gaagaagagt attaaatcga taataacatc
     2461 gaccggatcg aaacccggcc ggtaagcata gcttgataag gcaggactca atgacaactt
     2521 attacgtaga cggaaattcg ggcaatgatt cgaataacgg cagcagcgat agcccatgga
     2581 aacggctagg gaaggcgctt tcgaaagttc agccgggcga cgaggtacga gtccgaacag
     2641 ccacctacaa cgaggaatta acgcttacag tccgcaacac tacctggaaa gcagatacag
     2701 gccacaagcc ggtgctcgac ggccgctatc atgaaggcct ctttcgccct gacaggacgc
     2761 tgcccactcc cgtgcccggt tccggctatc tgcccgacaa actggggaac atggtcacgc
     2821 ttcgggaaga aggggtcatc ctcgatggct tcacgattca aaactgctcc ggcacaggaa
     2881 tcggggtcac cagcagcaac accatcgttc gcaattgccg cgtcgacttt acctacgact
     2941 cctcgattaa ggtgaatgcc accgccttta tcgataacgt tatcgtcgag aataacgtct
     3001 gcacccgctc cagcatgcgc tatttcgatc cgggtcgaaa ccagaccggt ggccctcaaa
     3061 gcgtgaacgg cgtgatgaag atggggcgga cacgcgacgg gatcattcgc aacaatatct
     3121 ttgcctatgg tcacggcgag ggaatcaaca tcggcaaagg caactatcgc atattggttg
     3181 aggggaacgt agtccataca tgtaatcacg tccatattta tatcaatcgc tcaatcgaca
     3241 ccatcatccg caacaacctc gtttatcatt tgtatactcc cgactacttg ggccctaaca
     3301 tgcgcccacc ggcgggtatc atttacggag atgagtatgc tcaaggccga ccatgggaaa
     3361 atagctcagg tgggcaagtc tataacaaca ttgttgttgg cttgggtcgg ctattcatgg
     3421 ttcgtaataa cgaaaacaac tacgacaccc aactcgataa cgcctacatc ggcttcaata
     3481 cgtttattgg cggcccgcta accgagattg gcattcagat ttccggcaat tataagggtc
     3541 ggccgcacaa aaacagcctc tttgaaaaca acatcataac caatgtgtca gtgatcagtc
     3601 aggctagtgg cgacttgaaa ggtatcttct ttcgaaacaa cttgtgggat gagcagccac
     3661 atgcggcttt gcgcgggcca ggcgatcgga taggcaatcc caatcttgtt aacccaaatg
     3721 ccgagatcaa agacctgttc cctgatcccg gcaataacgt cgatcctcgc aactaccaac
     3781 taacatcgcg tagttcacta gcgattggca tggccagcga cagctcacaa gccaacagta
     3841 ttaaaccgcc ggctgtcacc aaggacttct tcggtgcaaa tagggataac aaacccgata
     3901 tcggtgccca cgaatacgct ggagtcatca cggcactgac cgctaacttt agcattggcc
     3961 cgagccagga cgccggttcg atcccccaca cggtcgactt catcgacaag agtatttcag
     4021 aaggtgtcat catgtcgcgg ctatgggact tcggcgatag cggcacatcg tccgagacca
     4081 atccatccca tacctatacc acagcaggca cctacgacgt gacattgacg gttaccgacg
     4141 ataagggcaa caccgacacg atggtccaaa gcgggctaat cacagcacta gcggaaccgg
     4201 acacctccat ccctgactct ttccgtcgtt tcatcctcat ccagaaggac aatcagcaag
     4261 tcatggctta cgggacccag tacccggacc ttcgttgcgt ggttatttgg aaggttgatc
     4321 cattccatct aatgaacttc aacgacatag cagatgtcgt gcgcaacgtt cacgtagccg
     4381 atacgaatga agtcctgtgg atcgatcctg gcgatttaga tgagccggtg ttgacttatg
     4441 acgattcaga gatgtttggg cagtcgatgc cgacctcaac cttgcttcgt ggctaatagc
     4501 caccattgtt cagcaaatcc aaagaggcgt cccaacgcct ctttttttat ctgtcaggac
     4561 cactctagtt cttccaaatc gacagatctc gattgatcat actctccagc ttttcgatgt
     4621 cctcacgata aaaatcatac agccttcgaa caatggggag tggtatcggt ggcggctctt
     4681 ccaagttgtt attacgtgtg ttaatcatca tttggcgcag cttcgccggc acgtaagggt
     4741 cgatagcttg ccggagcgga tgagtcttaa tctgctcata aacgcgtccg agccgccgat
     4801 tcttcatggc cccgccctta ttgcgaatca ccagatcggg cacaaatgcg tcgtcaactt
     4861 ccaggaaccg acatagattg gcaaacaccc ccagcgtatc gcttattaaa tcttcgaaga
     4921 ggatgatctt gatctgcgcc gggtcgaaca gggcgttcca tttttcgaga gactgactgt
     4981 agaggccatg ttgcaccgcc tgatcctgtt ccatgagtgc atcctctacg ggccgatctt
     5041 cgatccccag ccgcacttta tgccaataac tagaataggc tcgcttaacc gggtcgcgca
     5101 ggctgaaaat tagtcgcacg tcagggatgg tctctttgat aagccttggc gcctgaggac
     5161 tgcgcagata atggggggag gactcgccga ttgcctgctc ggtcgtcgct tcttcaaata
     5221 atgcggcata ttctgtcaac gtacgaatgg ggaagcgcag cccgtgtcct tcaacatgat
     5281 cggggttgtc aggctcatag gcaaaataac gtggctcttt gaaacggctc atgaacacgt
     5341 caggatgttg cttcagatag tagttcaggg atgtagtgcc gcccttaata acaccgccga
     5401 tgataaagtt cggcaattgc atggtatctc cagcccattg aagtttctcg ttcccgtccc
     5461 gatggatcaa agatgtagag ttcattgtat tggccatcct tattagacaa accgcaactt
     5521 gccaatccgc atccgcgcgg attggcgcag ctgcttcatg tacatatcga tcatacccag
     5581 ctttagctct ggccattcgt catccggaag aacagcattg gttacgtcat gcgccgctaa
     5641 catctcctcg atcgaatcca gataagggct atcctggcgc agtctggcga tgtgggcttc
     5701 caatgtcgtc tcatcaggcc tttcgtagta cacctgtcga taattcctgt catcagcaac
     5761 atgatcgctt cgatggcctt tggcgcgctg ctgtacgtcc tgaaagcgat cgctctcctt
     5821 tgagtagaga tggatacgag acgacagatt gcctcccaac gaatggtgaa ctttctccca
     5881 atatcgctcc ttgccttcga agtagggtat gtcaaggcag gcacaggttg cctgcaagac
     5941 ggccgcctga tcgcgcgcat agtcggcata tttcacagta cggaaacctt ccacaaagga
     6001 ataaaaattg cgatgatacc gcggccaatt tgccagctcg gcaccatcct ttgcctccag
     6061 ccgattacgc ttcttaagcg aatgagcgaa ttccatcaag gtcttccaaa tcacgacgct
     6121 ctgaacttca atcccctgcc gcgacaggta gttgatctga tcatcgatcc aaaccacatt
     6181 cttgctggca tcaacgatga actccacctc aggatgaatc tcaaaaattg attcatagag
     6241 atgtgcctca ccgttcttct tgacccgatt ccagagagtg cattcaggat caccgcagcc
     6301 gcaagcccac gtcgggcgcg catgacgctc cttcgtgggg cgaaaaagat gtttgacctc
     6361 gccaacagca aaacctttgg ggtcgttagc aagagtcaga tgaaagaagg tggaaccact
     6421 ataggaagtg cctcctacaa atattacctt tctcgtcata aaacctctcg tgcgacccgt
     6481 ggccgcgcca tgacaatcta acccgatacg acttacagcg catattaagc ccaaggcagt
     6541 cgctcgccca tcgtggccta accggattac aattaccctg caccattact gcccttatta
     6601 taaggaacct catgcaaatc aaaccatttg ccacggaact cttttttaac ctctacgaat
     6661 tcaccacccc tcatctgctc tgcgcttccg attgcgagac gctgagcgtg agtgaattgc
     6721 tgcacctggc cggagccaac ccaagtgaac tgctggacct gcgactgggc tacacggagt
     6781 cgccggggca cccccattta cgggcggccg tctccaggct ctacgagcga acaagcgcgg
     6841 acgagatcgt gattttgggc gcgccggaag agggcatcta cctgactatg cgcgcgcttc
     6901 tggagccggg cgaccacgtt atcgtcctga ccccggccta tgattcgctg cttaacctgg
     6961 ccgagcacgc cagcggcaac gttagccgct ggccgattcg accgacctcc agcggctggg
     7021 cactcgatct cgaggagctg gggcgactca tcaccgatga tacccgtctc gttgtcgtca
     7081 attttcccca caatcctacc ggctacctgc ctgccgcgac tgaacttggc gcgctcatcg
     7141 acatcgcccg tcgccatggc gcctggctcc tgtgcgatga gatgtatcgc gggctggaac
     7201 gtaatccggc cgaccggctg ccttcggccg ctgatcgata tgagcgggcg atcgtcctgt
     7261 ccgggctgtc aaagacctac gggctgccgg gcttgcgcac cggttggctg gtcgtgggcg
     7321 acccggcaac gcgagcggcg atcatcaact ggaagcatta caccaccatc tgcgccgccg
     7381 cgcccagcga aacgctggcg accatcgcgc tgagcgccca cgaggctctc gcgatgcgta
     7441 accggcgaat catctccggc aatctcaagg cggccgatgc gttcttcagc cgttggccgg
     7501 acttgttcga ttggcggccg ccacgagccg gttccatcgc actcgtcggg atcgacgtac
     7561 cctcagccac cgaatattgt cacgcgctgg cccgcaatgc gggcgtgttg ctgctgcccg
     7621 gcccatgtct gggagccgac gaccacagcg tgcgcttcgg cttcggccgg cttgggttcc
     7681 aggaatcgct ggccgcctac gaacagtttc ttgaatcgga gagacaggat gccccggctc
     7741 gattttcaaa gccttgacga tctggccgct tggctcgcta catcgggcat cgatgtctcc
     7801 gagtggggaa atgacgacag caagaccacg gccgatctct ggcacgagtt tgtcgtcggc
     7861 gaatcttcct tcatcgacga ccctccaggc cggctggttg aagtagccca gatcttgatt
     7921 cgatggggag atgctatcct gatcgagatt gagcaggaat ttgtcgacgg ccgccgacga
     7981 gttcgcatgc ggccgccttc ggagaaactt gtgcgcggag aggagccgcg cacggccgcc
     8041 atccgctgcc tgaaagagga gttggggtta tcgtcggccg aagtgacggt tggggccgat
     8101 cacgagatta cagaagagag cgttgattcc ccctcatacc ccggcctgcc cacgcgctat
     8161 cgattctaca ccttcgaagt agccaccgac agcctgccga acggggactt ctgccgcgag
     8221 aacactgcct ccaatgaccc tattcgtcgc catctatggg gctggcgggc ggagtgggca
     8281 gtgaacagta gtcagtaggt cagtaggtca gtgaacagtg gcctgaccac tgacctactg
     8341 atccactgac ctactgatcc actggccact ctctcgcctg tccttcttac cagccgcgcg
     8401 tcgccagctg ctggtcttcg gccagggtat ggatgccgat gccgaccatc ggctggccca
     8461 ggttctcgct gacgcgggcc aggatttcgg cgttgcggaa gtgggtcgtg gcctcgacga
     8521 tggccttggc ccgccgcgcc ggatcgccgc tcttgaagat gccggagccg acgaagacgc
     8581 catcgacgcc gagctgcatc atcagcgccg cgtcggcggg ggtggcgatg ccgccagcag
     8641 cgaaattgac caccggcatg cggccgagtt cccgcgtctc cttcaccagc tcatagggcg
     8701 cgccgatctc cttggcgaag gtcatcagct cttcctcggg catattctgc aaacgacgaa
     8761 tgtcgcccag caccgtccgc gcgtggcgca cagcctcgac cacgtcgccc gtgcccgcct
     8821 cgcccttggt gcgaatcatc gccgcgccct cgccgacgcg ccgcagcgcc tcgcccagat
     8881 tccggcagcc gcagacgaac gggatcttga aatcattctt gttgatatgg tagctctcgt
     8941 cggccggggt caggacctcg ctctcgtcga tgaagtcgac gccgagcgcc tccagaatct
     9001 gcgcctcgac aaaatggccg atgcgcacct tggccatgac cgggatgctg accgcagcca
     9061 tgatctcctt gatcaggccg gggtccgaca tgcgggccac gccgccgctg acgcggatgt
     9121 cggccggcac gcgctccagc gccataactg ccaccgcgcc tgcttcctcg gcgatgcggg
     9181 catgttccgg cgtgacgacg tccataatga cgccgccctt gagcatctgg gcgaatcctt
     9241 ccttcacagt tcggctgccc acttcaccac ggccattaac gtttccattt tccttcatat
     9301 cgaatctccc ggccggccag cccatcagct aaaaccgccc ttcaatgaat ttattactgt
     9361 cgctaccatc tcattttaag agttgagaga aagaattaca aggatattgg ccccacccca
     9421 aggtgcgcaa catgatattt gatagagcca atttagcgga aacggcggag tggaaacgcc
     9481 tgaagtcttt ttctagcctg cctttcgatt gaaatcactt cttcagttca cttccgctcc
     9541 agggccacaa cttccctcgt aaaaggcttg cgcttcacac acctccccat tgggcaaagt
     9601 gcatgttccc cgttcggtag cagtgtctcc ttcctgggta atgtcgtatt gaccaccggt
     9661 gatggcgcaa aagcggcccg cttctgtgat gtaaccggtg atcttcagcc cgcccaccgg
     9721 gcactcacct cgcagcaaag cccactcctc acattgcctg ttgtccataa aatagcagac
     9781 gccaaattgt ccgccatcgg gacgtgattc caggttcagt atgccgcctt gctcgacaca
     9841 attggccgag gctggattag ccaactccgc agcaggtttg agtgccggtt cgattgttgg
     9901 ttcggctgtt gggttagcga ctgctccacc actgcaagca gccagcaaaa gcatcgttat
     9961 caggaacaga ggattaagtt tcatgatatt ttccctgatt ggccctccac atggacaagc
    10021 gttcgatatc ccatcacacg cttcaagcgt tgcagcaact cttgatcgtg aattatagaa
    10081 gtttcccgcc gattgcgatc tgaacaccaa caaatcgttc agaattacga cctgcactac
    10141 ccgaacgcgg ccgtttgtgc tcgatcttcc cttgtctaca agggccaaag gtttgctacc
    10201 ggtcatcggc tctcgtataa tccgaattgt tggatttatt cgatttattg gatcattttc
    10261 catgcaattc gataaactcg attccgaatt cctcaactac ctggtgacca accacgtcaa
    10321 cccgggtgat cgcctgcccg ctctgagcga gatcggcgac ggcatgggcg tcagcgtggg
    10381 caagctgcgc gagcaactgg ccgtggctcg cggcatgggc gtcgtatcgg tcaaacccag
    10441 gctcggcatc cagcgcgagc cgttcaactt cgccgaaagc gtgctgccgg ccgtgatgtt
    10501 cagcctcgct accggcgaag ccaggttcac ccacctcagt cgacttcgcc aggtgattga
    10561 ggtaagcttc tgggacgagg cggtcgtcct gctgacgccg gatgacaagg cgcgactgcg
    10621 tcaaatcgtg gccgatgcct gggacaaact gcgcggcgaa cccattcaca ttcccaacgg
    10681 cgagcatcgc cggctgcata tgaccatttt tagtcacctg aacaatccgt tcgttcaggg
    10741 atttcttgtt gcctattggg atgcctatga tgccagtgaa ttaacccgtt ttatgcgcta
    10801 tcgctattgg gtcgaggtgt ggagctatca tgaacgtatc gttgacgcgc tctgcgccga
    10861 tgattttgag tgcggccggc aactgctgat cgagcatttt tcgctgttgc cgaccgaacc
    10921 aggagcggaa agttaatcgg cattgagcgc cggttaacgg attagactta tcatcaaggg
    10981 gagtggaaca atgactttac gagaggagtt ggaagccgag caggtccggc atctggatct
    11041 gca
//
LOCUS       DAOMQG010000081        10952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2561_length_10952_cov_9.848399, whole genome shotgun sequence.
ACCESSION   DAOMQG010000081 DAOMQG010000000
VERSION     DAOMQG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2561_length_10952_cov_9.848399"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..731
                     /gene="radC"
                     /locus_tag="PLC06_06470"
     CDS             51..731
                     /gene="radC"
                     /locus_tag="PLC06_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRO90124.1"
                     /translation="MIRDMAEHDRPRERLIKVGAEVLSTAELLAIVLRSGIPGENVLR
                     LSERLLIRFDNLPGLARASITELIEANGIGPAKAAEIKAALEIGRRLVASAPEERPHV
                     SSPDDAFHLLKSEMMFLEQEHLRLILLDTRNRVLRTPTIYVGSLNTSVIRVGELFRAA
                     IRENAAAFIVAHNHPSGDPSPSPEDINVTRQIVQAGKLLDIDVLDHIVIGRNRYVSLK
                     QRGLGFEK"
     gene            complement(743..2014)
                     /locus_tag="PLC06_06475"
     CDS             complement(743..2014)
                     /locus_tag="PLC06_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90125.1"
                     /translation="MTESHRYSRQRLAWFILSASFFTCLLLTIAIPVAINTYLQNAGQ
                     SLDVTVQANQGTVGIEEPNGPRRAVLVGEPAQTISSQASIRTDTTASALMMVMLPNQV
                     EPLLTMQVASNSTIQMEGATAPRFRLSDNSNQVNIDLQAGRIRLDVPPLSDRPLDITV
                     TTPQDGRVLVAEPGRYMLEVSNDATQAHAFGPGQVTVTAQGQSLTLGPGQRAEIALGS
                     PPQGPLDPARDLIRNGDFSQGLDNWVLFSWQVELPDQPKGETTVQPDGGDPALRLTRQ
                     GVGHADVRLTQTVNQDVSDYETLRLAATLRIVDHSLGVCGVQGSECPLFFIINYIDDT
                     GVNRVWQHGFFANGTIDDNATPGACISCAVVQRPHDRVPLNQLYFYDVDLIQELASQG
                     ALPPRYIESLTIVGSGHSFTTEVSDVALIVE"
     gene            complement(2011..2289)
                     /locus_tag="PLC06_06480"
     CDS             complement(2011..2289)
                     /locus_tag="PLC06_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRO90126.1"
                     /translation="MSVRMEATVTGLVQGVFFRQSTLYTAQRLKLVGWVANRPDGAVW
                     VVAEGDEDVVQQFLAYLHDGPPAARVEKVEAKWSAATDEFPDFRVRYL"
     gene            complement(2372..3427)
                     /locus_tag="PLC06_06485"
     CDS             complement(2372..3427)
                     /locus_tag="PLC06_06485"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRO90127.1"
                     /translation="MTKSYYEQLFFPALKRLDPEAAHDHTLTALALAQSNPAGRAVLR
                     AIAGEVPARPVQVGALHFPNPVGIAAGFDKDAHVVAGLALLGFGHVEVGTLTPRPQPG
                     NPRPRVFRLPADGALINRMGFPNAGVEAAVARLGALPAKRDYVVGVSLGKQKETTLDE
                     AAEDYAIVMRAVHACADYLAVNISSPNTPGLRQLQGGRYLHELLAALVAENGRLSTMA
                     GCDPKPLWVKIAPDLELNGLDEILDTLLMAGIDGIIVANTTLRRDGLVDEARSEAGGL
                     SGRPARQRSTELIAHVYRRTNGTLPIIGVGGIDSAAAAREKLDAGASVVQLYTGLIYG
                     GPGIAGRIIRELAESDS"
     gene            complement(3420..5234)
                     /locus_tag="PLC06_06490"
     CDS             complement(3420..5234)
                     /locus_tag="PLC06_06490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRO90128.1"
                     /translation="MTNNLWDAAYRRDAWQNLTNENWDVVIIGGGITGAGLLREATRL
                     DLRTVLIEQRDFAWGTSSRSSKLVHGGLRYLKTGQVGLTRASVQGREQLLAEGPGLVA
                     PLGFLLAQYESDGAAAGRLVYGAGLTIYDLMAAQWSHRYYGPQDFQMMAPYISPTGLR
                     GGYQYHDAQTDDARLVLRVLRDAAAAGGTALNYVRAEEILLDDSGSATGVRLRDVLSD
                     SMVDVAARVIINATGAWADTLRVQMGAEPRIRPLRGSHLIFPAWRFPLAQAVSFMHPL
                     DGRPVFAFPWEGVTLVGTTDVDSSLPMDADPGITGEEVAYLMAALTACFPALGLSLDD
                     VMATFAGIRPVIGSGKLDPSEESRDHVIWEENGLLTVTGGKLTTFRQVARQALEAVCR
                     RIDGAGTETGQSCLERLHEDAPIFSSVGEDVPVLAALTDETRSRLLGRYGLDAAALAA
                     AAHEGELEAIPGTVVLWAELRWSARAEAVAHLDDLLLRRARLGLLLPNGGANHLPHIR
                     GIVQPELGWDDARWEAEEAAYRALIAASYSLPDRQAIPDWTDMLTRHQVEKGVAIVAH
                     QTRRGQIRRRAGLSLLAVGIPLFAIWLLKRGRKWVDHD"
     gene            complement(5231..6856)
                     /locus_tag="PLC06_06495"
     CDS             complement(5231..6856)
                     /locus_tag="PLC06_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRO90129.1"
                     /translation="MRRWNGWGDDTIQYPLTPRALAFLREAVGEARPPRDSTLAEVLA
                     TVPPSRLRDLPGRDLVHVDERERLKHARGQSLPDWVALRTGHVPTFPDGVAYPSSAGD
                     VARLLEYAARVDARVIPYGGGTSVVGHINPEPGEQPVLTVNMRRMNQLTRLDSISQLA
                     TFGAGVAGPELEAQLRARGFTLGHYPQSFEFSTLGGWVVTRSSGQQSLGYGRIENLFA
                     GGVLLAPAGEMRLPPFPASAAGPDLRQLVLGSEGRLGILTEATVRVSPLPEHEEFHAV
                     FFPDFISGTAAVRRIVQARLPLSLLRLSTPVETRTTLALAGHERLIGLLETMLGVRGV
                     GDGKCMLLCGFTGQSKTVRDARQAVMEIAKDHAGVSVGRRFGDQWIEGRFRTPYLRNT
                     LWELGYAVDTLETATTWDGVPRMVEVIEEALHSTLPNGERVHVFTHLSHVYSQGASVY
                     TTYLYRLATDPDETLRRWELLKGNASRAIVAAGGTISHQHGVGRDHAPYLQAEKSALG
                     LAALRGAFAEFDPDGRMNPGVLLGRRAEGGQPR"
     gene            complement(6861..7574)
                     /gene="fadR"
                     /locus_tag="PLC06_06500"
     CDS             complement(6861..7574)
                     /gene="fadR"
                     /locus_tag="PLC06_06500"
                     /inference="COORDINATES: protein motif:HMM:NF003444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HRO90130.1"
                     /translation="MNHRTSHIRPSEHAESALVTAILDGEFPPGSALPGERVLAGQLG
                     VTRPTLREAIQRLARDGWLTVAHGKPTLVNDFWRKGGLNVLGRLIEHQAHLPADFIPN
                     LLEVRLQLAPTYTRAAISRVPAVILDLLASAPDIEDPPVTFARFDWQLHQQLTIHSGN
                     PIYTLILNGFSGFYEDIAQIYFAENGARELSVAFYEDLRQAVTNADPAAAEALTRRVM
                     MASLDVWRGEAMTTEKIEE"
     gene            complement(7711..7992)
                     /locus_tag="PLC06_06505"
     CDS             complement(7711..7992)
                     /locus_tag="PLC06_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90131.1"
                     /translation="MSGERGPVSSQFGLKLVGSMNPADFDFFPGLELAAFLAFFSLPV
                     ELFLVVMIVVSPDAHDNRPWVLVGATLITPSWKIAAQRTIADEKDVNLG"
     gene            7991..9622
                     /locus_tag="PLC06_06510"
     CDS             7991..9622
                     /locus_tag="PLC06_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90132.1"
                     /translation="MLSIPATGQEEAPPLTPDEFDALQESLQRYYREVSGDEPEEIAQ
                     AEPIESIAFTPAEETDPVAEEPVELPTSDIATEIIEEPDPAPQIAIAEPEAWIAPLLD
                     PAAKSSAEPEPTEIATLPEPTEDIPLSDQFSEPAEDIPPLGQLVEETPVAIATTTTVA
                     VAPDAKTEKSSEAARPKTKKRRDRLSWALFLLSILILGAAALIYFVNPFSRLALGAAS
                     LARPVASPELPSPTTGSGAWCLSGEFLSGSGDKTQLTDSGASGDILAEDSVYSLDYAI
                     ASPGSYKWQVIDCNNEALAYPAAPAWVTTTEPNQVVTFNFDSTERSDPLFFPIPHVVS
                     ALDDTDDYQIIGDFQGWNPDDASGKMQKLNLGLFQHVRRIARSGSYEGYVIAGDREHA
                     IDAYGRTTDPIPFSFTTERNGEYVVFLVDTDRGRASIMYDMPPVLTSLAYGSGNWMLS
                     GALVILAGLLLIGVILRRLILHNKNLQMESGCPNCGRHELMRISRRPSDRVLHMMGIP
                     AYRYRCRNCTWEGMRLSEEGTAVSAGVPLAFYERD"
     gene            9647..>10952
                     /locus_tag="PLC06_06515"
     CDS             9647..>10952
                     /locus_tag="PLC06_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90133.1"
                     /translation="MREATCRVTISLMGYSRFRTKLPVILLGLVLALSACVRPGPSRT
                     AIGTSISEGQQGALPTIPAINDDNPRPLIEQLGTPLPDYIGQPTADAPHYEISIAPEG
                     TLGAHTVAAGETLGTLAQRYSTSIEELMELNNLSDPDLLHVGQVLQVPGGGIQQITGP
                     EFKIIPDSELVYGPAAKGFDARLFITNLNGYLARHQEEVEGLPMDGASTLQLVADRHS
                     VNPRLLLALLEHQTGWVTQATPPSTTPNLGYSGAGASGLYGQLSWAANLLNLGFYGRA
                     EGGLRSLNLGETTQVMFSPTINDGTAGVQRFFAAVDGATYESWQKELGQDGFFATYSR
                     LFGNPFAYTFDPLLPAGLSAPEMELPWAVGETWYFTGGPHGAWNSGSAWGALDFVPPG
                     EQVGCIQSEDWVTSATGGTVTRSDHGAVVVDLDGDGYAGTGWA"
BASE COUNT         2382 a         3439 c         3082 g         2049 t
ORIGIN      
        1 gatcaggata tatgatggaa acagaacggg aaacagcgcg ctatgtgccg atgatccgtg
       61 atatggccga acacgatcgg ccgcgcgagc gattgatcaa ggttggagca gaagtgctgt
      121 cgacggccga gcttctggcc attgttctgc gcagcggcat ccccggcgaa aacgtgctgc
      181 gactgtcgga gcgattgctg atccggtttg acaacctccc cggtctggcg cgcgcttcca
      241 tcactgaact catcgaggcc aatggcatcg gccccgccaa ggcagccgaa atcaaggccg
      301 ctctggagat cggccggcgc ctagtggcca gcgcacccga agagcggccg cacgtttcat
      361 ctcccgatga cgcctttcac ctcctgaaat ccgagatgat gttcctggag caggagcatt
      421 tgcgcctcat ccttctggac acacgcaacc gcgtactgcg cacgccgacg atctatgtgg
      481 gcagcctgaa cacctccgtc atacgcgttg gggagctttt ccgggctgcc attcgcgaga
      541 acgccgccgc attcatcgtg gcccataacc atccctcagg cgatccgtct ccctcgccgg
      601 aggatatcaa cgtcacccga cagatcgtgc aggcaggcaa gttgctcgat atcgacgtgc
      661 tcgatcacat cgtcatcggt cgcaatcggt atgtctctct caagcagcgg gggctgggtt
      721 tcgaaaagta agcgcggctg gtttattcca caatcaaggc cacatccgac acttcagtcg
      781 tgaagctgtg gcctgagcca acaatggtca ggctctcaat atagcgcggc ggcagcgcgc
      841 cctggctggc caactcctgt atcaggtcga catcgtagaa atagagctgg ttcaagggta
      901 cgcggtcatg cggtcgctgg acgacagcac aggagataca cgcgcccggc gtcgcattgt
      961 cgtcgatggt gccattggca aaaaagccat gctgccacac gcgattgacg ccggtgtcgt
     1021 cgatatagtt gatgatgaaa aacagtgggc attcactgcc ctgaacgcca cacaccccca
     1081 gactatgatc gacaatgcgc agcgtggccg ccaggcgcaa cgtctcataa tcggagacgt
     1141 cctgattcac agtttgggtc aaccgcacat cagcatggcc cacaccttgt cgtgtgagac
     1201 gcagtgccgg atcaccgcca tcaggctgca cggtagtttc acccttgggc tggtcgggca
     1261 actccacctg ccaggaaaac aggacccaat tgtccaaccc ctggctaaaa tcgccgttac
     1321 gaatgaggtc acgcgccgga tcgagcggcc cttgcggtgg ggaacccagc gctatttctg
     1381 cccgctgccc gggacctaat gtcaacgact gtccttgtgc agtgaccgtc acctggcctg
     1441 ggccaaaagc atgggcttgt gtcgcatcat tgctgacctc gagcatgtag cggcccggct
     1501 cggcgactaa cacgcgccca tcctgggggg tggtcaccgt gatatcgagt ggccgatcag
     1561 agagcggagg gacatcgaga cgaatacgac ccgcttgcag gtcgatattc acctgattgg
     1621 aattatcact caacctgaac cgcggcgcgg tcgccccttc catctggatg gtggaattgc
     1681 tggcgacttg catggtgagg agcggctcaa cctgattggg taacatcacc atcataagtg
     1741 ccgacgctgt cgtatcggtg cgaatcgagg cctgagagga aatcgtctgc gcaggctcgc
     1801 ccaccaggac ggcgcgccga gggccattcg gctcctcaat gccgactgta ccctgattcg
     1861 cctgaacggt aacatccagt gattggccgg cattttgcaa ataggtatta atcgccaccg
     1921 ggatggcgat tgtcagaagg agacaggtga aaaagctcgc cgagagtatg aaccaggcca
     1981 atcgctgccg gctataacga tgcgattcgg tcatagatag cggacccgga aatcaggaaa
     2041 ttcgtccgtg gcggccgacc atttggcctc cactttctct acgcgagcag caggaggccc
     2101 atcatgcaaa taagccagga actgttgcac aacgtcttcg tccccttcag ccacgaccca
     2161 caccgcacca tcagggcgat tcgctaccca ccctacaagt ttcaaccgct gggccgtata
     2221 cagggtagat tggcgaaaaa acacgccctg aacaaggccg gtgaccgtgg cttccatacg
     2281 aacagacatg ggcaaattat agcgcagtct cgtggttcgg ctattgagca acggccacta
     2341 gtgggtgagc agtagaccga acgaagggtc ttcaggaatc gctttcagcc aactcgcgga
     2401 tgatccgtcc ggcgatcccc ggcccgccat aaatcagacc agtgtagagc tggacgaccg
     2461 acgcccccgc atcgagcttt tcccgtgcgg ccgcggccga gtcaatccca ccaacgccga
     2521 taatgggcaa tgtgccgttc gtgcgacgat agacatgcgc aattagctca gtcgagcgtt
     2581 gccgcgccgg ccggccgctt aacccgcccg cctcagatct ggcctcatct accaagccat
     2641 cacgtcgcaa agttgtgttg gcgacaataa tgccgtcgat gccggccata agcagcgtat
     2701 ccagtatctc atccagcccg ttcaattcca ggtccggcgc gattttgacc cacagcggct
     2761 tgggatcgca gccggccatc gtcgagaggc ggccgttctc ggccaccaac gccgcgagta
     2821 gttcatgaag gtagcggccg ccctgcaatt gtcgcaatcc gggcgtattg ggcgagctga
     2881 tgttgaccgc aaggtaatcg gcacaggcgt ggacggcgcg catgacgatg gcgtaatctt
     2941 cggccgcctc gtccaacgtg gtctcttttt gcttgcccag actgacgccg accacataat
     3001 cccgtttggc cggcaacgcc cccaggcgcg ccacagccgc ctcgacgccc gcattaggaa
     3061 aacccatgcg gttgatcagg gcgccatccg caggcaaacg aaagacccgc ggccgtggat
     3121 tgccgggctg cggacgaggt gtcagcgtgc ccacctcaac atggccgaac ccgagcagcg
     3181 ccagcccggc caccacatgg gcatccttgt cgaatcccgc ggctatgcca accgggttag
     3241 gaaaatgcag tgcgccgacc tggaccggcc gtgccggcac ctctccggca atggctctca
     3301 acactgcgcg cccggccggg ttcgattgag ccaaggctaa cgcggtcagc gtatgatcgt
     3361 gcgccgcttc agggtccaac cgcttgagtg cggggaagaa cagctgctcg taataggatt
     3421 tagtcatggt caacccattt tcgccctcgc ttcagcaacc atatggcaaa caagggtatc
     3481 ccaaccgcaa gcagcgacaa tcctgcacgg cgtcggattt gtccccgacg cgtttggtgt
     3541 gccacgatgg ccaccccctt ctcgacctgg tgacgtgtca acatatccgt ccagtcaggg
     3601 attgcctgtc tgtcaggcaa tgagtaggaa gcagcgatca gcgcgcgata agcagcttcc
     3661 tcggcctccc aacgcgcatc atcccagcca agttccggct gaacgatgcc gcgaatatgg
     3721 ggcaggtgat tggccccacc attgggcaga agcagaccca agcgcgcccg acgcagcagc
     3781 agatcgtcaa gatgtgccac cgcctcggct cgcgccgacc aacgcagttc ggcccacaga
     3841 acgaccgtgc ccggaatcgc ctccaactct ccttcatggg cagcggctgc cagtgccgcc
     3901 gcatccaaac cgtagcgacc gagcagacga cttcgagttt cgtccgtcaa agcagccaac
     3961 acgggcacgt cctcccctac agaactaaat atcggcgcat cttcgtgcaa gcgttcgaga
     4021 cacgattgcc ccgtctccgt acccgctcca tcaattcggc ggcagaccgc ttccagggcc
     4081 tgccgcgcga cctgacggaa ggtcgtcagc ttgccaccgg tgacggtcag caggccattc
     4141 tcctcccaga tgacatgatc gcgagactcc tccgatggat cgagcttgcc gctgccgatc
     4201 accggccgaa tacccgcaaa agtcgccatc acgtcatcaa ggctcagccc caatgccggg
     4261 aagcaagcgg tcagggcggc catcagataa gcaacttcct cgcccgtgat gccggggtcg
     4321 gcatccattg gcagagaact gtcgacgtcg gtcgtcccga ccagggtcac accttcccac
     4381 gggaaagcga acaccggacg gccgtccagc gggtgcatga aactgacagc ctgcgccaac
     4441 ggaaagcgcc aggcggggaa gatcagatgg ctgccacgca gagggcgaat gcgaggttcg
     4501 gcccccatct gtacgcgcaa cgtgtcggcc caggcccctg tcgcattgat gatgactcgc
     4561 gccgccacat cgaccatgga atcgctcagg acatcgcgca agcgcacacc tgttgcgctg
     4621 cccgaatcat ccaggaggat ttcttcggcg cgaacatagt tgagcgcagt ccctccggcg
     4681 gcagcagcat cgcggagaac ccgcaacacc agacgggcat cgtcggtctg ggcatcatga
     4741 tactgatacc cgccacgcag accggttggc gaaatatagg gggccatcat ctggaaatcc
     4801 tgtgggccat aatagcgatg gctccattgc gcagccatta aatcgtagat cgtcagcccc
     4861 gcgccataga ccagccggcc cgcggccgcg ccgtcacttt catattgggc cagcagaaaa
     4921 ccaagcggag ccacgaggcc aggaccttcg gccagcagtt gctcccgccc ctgtaccgac
     4981 gcccgagtca accccacctg ccccgtctta aggtaacgca acccgccgtg aaccagcttc
     5041 gaggaacgac tggacgtgcc ccaggcgaaa tcgcgctgct cgatcaacac ggtccgcaaa
     5101 tccagccgcg tcgcctcgcg cagcagtccg gccccggtga ttccaccccc gatgatgacc
     5161 acatcccagt tctcattagt caggttttgc catgcatccc gacgataggc tgcatcccac
     5221 aagttattgg tcatcgcggc tggcctcctt ctgctcgacg accgagcagc acgccggggt
     5281 tcatgcggcc gtcaggatca aattcggcga acgcaccacg aagagcagcc aggccgagcg
     5341 cgctcttttc tgcctgcaag tagggcgcgt gatcgcgtcc aacgccgtgc tgatggctga
     5401 tcgtccctcc cgcagcgacg atcgcccggc tggcgttccc tttcaacagt tcccagcggc
     5461 gcaacgtttc gtcgggatct gtggcgaggc gatacagata ggtcgtgtag acgctggccc
     5521 cctgcgaata gacgtgggac agatgggtga agacatgaac ccgctcgcca ttgggcaaag
     5581 tcgagtgcag agcttcctcg ataacctcca ccatacgcgg cacaccgtcc cacgtggtgg
     5641 cggtctccaa cgtatccacc gcatagccca actcccacaa tgtgttgcgc aaatagggtg
     5701 tgcggaaccg gccctcgatc cattgatcgc cgaagcgacg gccgacgctc acacctgcgt
     5761 gatctttggc gatctccatc acagcctggc gagcatcgcg aaccgttttc gattgcccgg
     5821 tgaagccaca gagcaacatg cacttgccat cgccgacgcc gcgaacgccc aacatcgtct
     5881 ccagcaagcc gatgagccgc tcgtggccgg ccagagccaa tgtcgttcgg gtctccaccg
     5941 gtgtgctcag tcgcaagagc gagagcggca accgtgcctg gacaatccgg cgcacggccg
     6001 ccgtgccgct gatgaaatcg gggaagaaga cagcatggaa ctcctcgtgt tccggcaacg
     6061 gagagacccg aactgtcgct tcggtcagga tgcccagccg cccctcggaa ccgagaacga
     6121 gttgacgcag atcggggccg gcggccgacg cgggaaacgg cggcaaacgc atctcccccg
     6181 ccggagccaa cagcacgccg ccggcaaaca ggttctcgat acggccatag cccaatgatt
     6241 gctgtccact ggagcgagtg accacccagc cacccaatgt tgaaaattca aaggattgcg
     6301 gataatgacc cagcgtaaag ccgcgcgccc gcagctgggc ttccagctca ggaccggcca
     6361 cgcccgcgcc aaaggtcgcc aactggctga tcgaatccag gcgcgtcagc tgattcatgc
     6421 gtcgcatgtt gacggtcaag actggttgct cgcccggttc ggggttgatg tggccgacga
     6481 cgcttgtccc acccccatag gggatgaccc gcgcgtccac ccgggcagca tactccagca
     6541 gtctggccac gtctccggcc gatgatggat aggcaacgcc atcgggaaaa gtcggtacat
     6601 ggccagttcg cagcgccacc caatcaggca agctctggcc acgagcatgc tttaatcgct
     6661 cgcgctcatc aacatggacc aaatcgcggc ccggcagatc gcgcagacgc gacggcggga
     6721 ccgtcgccaa tacttcggcc aacgtggaat ctcgtggcgg cctggcctcc cccactgcct
     6781 cgcgcaggaa tgccaacgcg cgtggcgtta gcggatactg tatagtgtcg tctccccaac
     6841 cattccaacg acgcatggct tcactcctcg atcttttctg tggtcatcgc ttcgccgcgc
     6901 caaacatcca gactcgccat catgacccgg cgggtcagcg cttcagccgc tgcgggatcg
     6961 gcattagtca cggcctgccg caaatcctcg taaaaggcta ccgacagttc acgcgcccca
     7021 ttctcggcaa aatagatttg ggcaatatcc tcatagaatc cgctgaagcc attcaggatg
     7081 agcgtgtaga tggggttgcc ggaatggatc gtcagctgct ggtgcagttg ccaatcgaaa
     7141 cgggcaaacg ttaccggtgg atcctcaata tcgggtgccg aagccaacag atccaaaata
     7201 accgccggaa cacgtgaaat cgccgcgcgt gtgtaagtag gggccagttg caggcgcact
     7261 tcgagtaagt tggggatgaa atccgccggc aaatgtgcct ggtgttcgat taacctgccc
     7321 aacacattta atccaccctt gcgccaaaaa tcattgacca gcgtcggctt gccatgagcc
     7381 acggtcagcc agccatcccg agcaaggcgc tggatagctt cgcgcagcgt cggccgcgtc
     7441 actcccaatt gacctgccag cactcgctcg cccggcaagg cagaacccgg cgggaactcg
     7501 ccatcaagga tcgccgtgac taacgcactt tcagcatgtt cgctgggccg gatgtgggag
     7561 gtccgatgat tcatggatga tctttggagt ggtctgacca gtcaactata tcataccgaa
     7621 aggaaaagcg taatgtcaag cctctatgat gagaaacagt tttattggtc aatgtgacca
     7681 tacagggacg ttcgcatctt cacgtagaat tcacccgaga tttacatcct tttcatcggc
     7741 gattgtacgc tgggcagcta tcttccatga tggggtgatt agcgtagccc caaccagaac
     7801 ccacgggcga ttatcgtggg catcgggact tacaacaatc atgacaacta aaaacagctc
     7861 taccggcaac gagaaaaacg caagaaacgc cgccaattcc aaccccggaa agaaatcaaa
     7921 gtcggcggga ttcattgagc cgaccagttt caaaccgaac tggctcgaga ccggccctct
     7981 ttcacctgac atgctttcga ttccggcaac gggtcaggaa gaggcaccac cgctaactcc
     8041 ggatgaattt gatgcgctcc aggaatcatt gcagcggtac tatcgagaag tatcagggga
     8101 cgaaccggag gaaatcgcac aagccgaacc gattgaatcc atcgctttca cccctgctga
     8161 ggaaaccgat cctgttgcag aagagccggt cgagcttcca accagcgata tcgccacaga
     8221 aatcattgag gaaccggacc ctgctcccca aatcgcaata gcagaacctg aagcctggat
     8281 cgccccactt cttgacccgg cagccaagtc gtcggccgaa cccgaaccaa cagaaattgc
     8341 aacgctaccg gaaccaaccg aggatattcc cctgtcggac cagttctcgg agccagccga
     8401 agacattccc ccattgggcc agctcgttga agaaactccg gtagcaatcg caaccacgac
     8461 taccgtagcg gttgccccgg atgcaaagac ggaaaaatcc agcgaagcag ctcggccgaa
     8521 aacaaaaaaa cggcgcgacc gtctgtcatg ggccttattc ttgctcagca tcctaattct
     8581 gggcgcggcg gcgttaatct atttcgtgaa tcctttttcc cgactcgctc tcggtgcggc
     8641 gtctttggcc cgcccggttg cctcgccgga gctgcccagc cccacaaccg gaagcggcgc
     8701 gtggtgtctg agcggcgaat tcctgtcggg atcaggcgac aagacacaat tgaccgatag
     8761 cggcgcatcc ggcgacatat tggccgaaga ttcggtctat tccctggatt atgcgatcgc
     8821 gtctccgggg tcttataaat ggcaagtaat cgattgtaac aacgaagctc ttgcttatcc
     8881 cgccgcgccg gcctgggtca ccacgacgga acccaaccag gttgtaacat ttaacttcga
     8941 cagcaccgag cgatccgatc cgctcttctt ccccatcccc catgtggtta gcgccctgga
     9001 tgatacggac gactaccaga tcatcggcga ttttcagggc tggaaccctg acgatgcttc
     9061 aggcaagatg cagaagctca acctggggct atttcagcac gtccggcgca tcgcgcgttc
     9121 cggcagctat gaagggtatg tcatcgccgg agaccgagag cacgctattg acgcctatgg
     9181 tcgcacgacc gatcccattc cattctcctt tacgaccgag cgcaacgggg aatacgtcgt
     9241 ctttctggta gacactgacc gcggccgcgc gtcgatcatg tatgacatgc ccccggtatt
     9301 gacttctctg gcctatggca gcggcaactg gatgctaagc ggtgcgctgg tgatactggc
     9361 cgggctgctg ttgatcggcg tcatcttgcg tcggctcatc ctgcataaca agaatctgca
     9421 gatggaatcg ggctgcccca attgcggccg tcatgaactg atgcgcatct cccgtcggcc
     9481 gtccgaccgt gttttgcaca tgatgggcat tccagcctac cgctatcgtt gtcgcaattg
     9541 tacctgggaa gggatgaggc tcagtgagga gggcaccgca gtctctgccg gtgtgcccct
     9601 ggctttctac gaaagagatt aattggaata agtagataac gtccgaatgc gagaagcgac
     9661 ctgtcgcgtt acaatctcgc tgatgggtta cagcagattc cgcacaaaac taccagttat
     9721 cttgctgggg cttgttctcg ccctcagtgc ttgcgtccgg cccgggccgt cacgaaccgc
     9781 gatcggaacc tccataagcg aaggacagca gggggcactc ccgaccatcc cggccatcaa
     9841 tgacgacaat ccccggccgc tgatcgagca gctcgggacg ccattgcctg attacatcgg
     9901 ccaacccacc gccgatgccc cgcactatga gattagcatt gccccggaag gcacgctggg
     9961 cgcccacact gtggcggcgg gcgagaccct cggcaccctg gcccaacgat attccacctc
    10021 aatcgaagaa ctgatggaac tcaacaatct aagcgatccc gatctactcc atgttggaca
    10081 ggttttacaa gttccggggg gcggaattca acaaatcaca gggccggaat tcaaaatcat
    10141 tcccgacagc gagctggttt atggcccagc ggcgaaagga tttgatgctc gtttatttat
    10201 caccaattta aacgggtatc tggcgcgcca tcaggaagaa gtcgaagggc taccgatgga
    10261 tggggcctcc accctgcagt tggtcgccga tcggcacagc gtcaacccgc gcctgctgct
    10321 ggccctgttg gaacatcaaa ccggctgggt gacacaggcg acgcctccct cgacaacgcc
    10381 taaccttggc tacagcggcg cgggcgcgtc gggcctctat ggtcagttga gttgggcggc
    10441 caatctgctc aatttgggct tctatgggcg agccgagggt ggcctgcgca gccttaatct
    10501 gggcgagacc acgcaggtca tgttttcgcc cacgatcaac gacggcactg ccggtgtaca
    10561 gcgtttcttc gccgccgtcg atggcgcaac ttatgaatcg tggcaaaaag aactggggca
    10621 ggacggcttc tttgccacgt attcccgact ctttggcaat cccttcgcct ataccttcga
    10681 tccgcttttg cctgccggat tgagcgcgcc ggaaatggag ctgccctggg cagttggcga
    10741 aacgtggtac ttcacgggcg gaccccatgg ggcgtggaac agtggttcgg cctggggggc
    10801 gctggatttt gtgccccccg gcgaacaggt cggctgcatc caaagcgaag actgggtgac
    10861 cagcgcaacc ggcggcaccg tcacccgcag tgatcatggc gccgtcgtcg tcgatctgga
    10921 cggcgacggt tacgcaggga caggctgggc ta
//
LOCUS       DAOMQG010000082        10931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2570_length_10931_cov_9.049283, whole genome shotgun sequence.
ACCESSION   DAOMQG010000082 DAOMQG010000000
VERSION     DAOMQG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2570_length_10931_cov_9.049283"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..867
                     /locus_tag="PLC06_06520"
     CDS             238..867
                     /locus_tag="PLC06_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90134.1"
                     /translation="MSSANLPPASGAYSSQQPSAGKPGSWAAYDQPPNEWPPSNAPYS
                     STTYRPTPEQLTRDELRQRYLRRNVYAPIIIAAILAVALFALIIYLAFGVATPQAKSF
                     IAGMSALVVILFAGPMIILMAILPIAWLAFRLNRRQKRKEFPETGPMAYRSRIQAWLW
                     QLDGLLDSAEHGVDRLGTRIRQPLIALHARFAWLRGWLNGIRGKFTRSI"
     gene            869..1126
                     /locus_tag="PLC06_06525"
     CDS             869..1126
                     /locus_tag="PLC06_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90135.1"
                     /translation="MSKSDRTRNQELAAMDQQESWQAKVLITGGIIGAAVGLATSWLL
                     IRTSRETRSGPPAITTGDAIKVGVTTIGLVRAIAALGDRRA"
     gene            1252..2985
                     /locus_tag="PLC06_06530"
     CDS             1252..2985
                     /locus_tag="PLC06_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRO90136.1"
                     /translation="MDLHLHTPASNDHQEPHVSYLDILRQAAARGLDIIAITDHNTVA
                     GFATMQKEVEQLLWLESRGRIDPAEQRLLDEYRRVLGKLLVLPGFEFTATFGFHILGI
                     FPPETPVSYLEHLLLTLRVPPASLRVGSSTVGATSDVLTAYHVINEAGGLVIAAHANS
                     GNGVMLRGLDFGGQTRIAYTQDVNLHALEVTDLEKRGNQTTRRFFDGSKPEYPRPMRC
                     IQGSDAHRLIRESANSPYLGVGDRVTEILLEEVSFDALARVIRGNDHSLTRPWRGTAK
                     AIDFVQSAREEGESLVQSFHPSINQRGGHQDSVLRDICAMANTNGGTIYVGIPADPKA
                     KPEGVREPHKAIETLQTLISKRFSPEPQVVIDSLPTQGATVMRISVTPGPDAPYAIDH
                     NQFYVRDESETTLAVRDEIVRMVERNLTGRVVEQKPPAEPVANPTPPPSSGRKSEPTS
                     RPTPANGRGGYEHPRTGVEHVDSTERDGVIYHTLRDLRNGNLINNVTRSSARRLWHYA
                     ITQVETAPPDLSVAQWRNNVAILNRRQKNNNTLYDLAVREGDRAHIYYGVTESGLSDA
                     LLALVNEQARE"
     gene            2982..3482
                     /locus_tag="PLC06_06535"
     CDS             2982..3482
                     /locus_tag="PLC06_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRO90137.1"
                     /translation="MRRVGILTISDRGAEGVYEDKSGPLAAGLVAERTGWTIALQTII
                     PDEFETITGTLREWCAAGLDLILTSGGTGFAPRDVTPEATRAVIEREAPGIAEALRAE
                     SLKVTRHAMLSRGVAGLRGATLIVNLPGSPKAVAENLDLLLPVLPHALDLLTGRVESG
                     QGHRDV"
     gene            3519..4436
                     /locus_tag="PLC06_06540"
     CDS             3519..4436
                     /locus_tag="PLC06_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRO90138.1"
                     /translation="MTSSPRLRANLLLLLTAVVWGFAFVAQRSGMAFVGPFTFNAVRF
                     LIGGLMLVPLMVVMDRRTTESGRPPVPLRDRNLLTGGVLVGLVLFGAATLQQMGVALT
                     TAGKAGFITSLYVVLVPLLGLALGHRSSAAVWLGAILAAAGLYFLTIEGGFYMAWGDL
                     LVLFGAFLWAGHVLLLGRLSPQTDTVKLAFLQFVVCAALSAAAALLTETISAADLRAA
                     LWPILYTGIMSVGVGYTLQTIAQAHARPADTAIILSLEAVFAVIGGWLLLGEVLSARA
                     LFGCALMLAGILISQLLGRPEDAAAEISH"
     gene            4454..5725
                     /locus_tag="PLC06_06545"
     CDS             4454..5725
                     /locus_tag="PLC06_06545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRO90139.1"
                     /translation="MTSTEQPTATLILKPGRDKPVHNRHPWVFSGAIGRVKGQPAPGD
                     LVTVADHKGTPLATAYYNAKSQIQARILSWEPAVPIDESFWRNRLQQAIHGRSLITTP
                     LITDSLNTDPLITVPLPTAPLNTAHRLINAEADLLPGLVVDRYGDYLVMQCLTLGIDG
                     RKEMLARLLAELLHPAGILERSDVDVRGKEGLRPLVELRHGQEPPAELTIRENGFSFL
                     VDLRHGHKTGFYLDQRDNRATAGQPALMAGREVLNVFAFTGAFGVYAAAAGARNVVQI
                     DSSAPALETAERNMKLNGLDKASDEYIAGDAFEVLRYFREEGRQFDAIILDPPKFAHS
                     QSQIDRAARGYKDLNWLAMRLLRPNGVLATFSCSGLVSADLFQKIVFGAAVDAGRDAQ
                     ILHHLGQSADHPVLLSFPESAYLKGLLCRVI"
     gene            5725..6600
                     /locus_tag="PLC06_06550"
     CDS             5725..6600
                     /locus_tag="PLC06_06550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO90140.1"
                     /translation="MRVSVIIPSLNAPTLGRALAAVVAQSEPPHEVIVVGRDEAGTLQ
                     THPAVQFIDTGVPVCAARARNLGMGAASGDLFLLLDSDCIPAPDWLARHRARHAAGET
                     VVGGAVALQGSNYWAQSDNVSMFHDFVPQLPAGPRFLLPTLNLSVRREVFAAVGGMDE
                     SFPGAAAEDADWTVRIRRAGHRLWFDPAAVVAHSPARVAPRDVVRHWRYLGHNAIRVR
                     LRYADEFKTPNGAQYAWWWRLFSPIIAAGVTAGIYAKRPLRRYWAGLPVVYATKIIYC
                     WGAARAIDSGFATQP"
     gene            6602..7525
                     /locus_tag="PLC06_06555"
     CDS             6602..7525
                     /locus_tag="PLC06_06555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO90141.1"
                     /translation="MPPVTVLIVAWNQLAKTLDCLATVAALDYPDFRVLLVDNGSEPP
                     LAEAIAARFPDVATLRLPGNLGFAGGYNAGLRQGLAGDGRYFLLLNNDTLLEPDVLSK
                     LVAEIERAAGIGLVTAKIYYAADRQRIWTVGANLNVFLDLKEGGGGQIDTGQWDSPRD
                     IDFAPFCALLIRREVLEEVGLLDEGFFLYYEDMDYCRRAKAAGRRVRLCPTAHVYHDV
                     SASSGGRDSPMERYWMAQSSGRYFRKHGRGLRLLLIIPFRLASAIKMTARLLVQGKHE
                     ALRAYWSGLIIGWRTGRATTPPKAKRAIEST"
     gene            7550..8464
                     /locus_tag="PLC06_06560"
     CDS             7550..8464
                     /locus_tag="PLC06_06560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90142.1"
                     /translation="MTYKRQMRQLAKRIIPKSPLVNTWAAWCRLKARHARRIFEEAGD
                     SPAWLGTAELDALQRAYPLEPLYYGYDPESIVARGRERAQAMLNLVHHSDRFSRFLDL
                     GMWDGMTCYALRELGVATTGIDIRVEGLDQRAVDSGAHFAAMDAAHLGFAAESFDFVF
                     SFNSFEHFPDPELVLRETLRVLRPGGYLYIDFGPLWLTPKGAHQFETITVPYVECLFS
                     KETLTAYATANDIPLKPYEWMNEWPLSRYRSLWQRYEGQMERVTYHEIYNADHVDLIA
                     RYPTCFKSKTRTFDDLIISNIEAVFRKR"
     gene            complement(8480..9184)
                     /locus_tag="PLC06_06565"
     CDS             complement(8480..9184)
                     /locus_tag="PLC06_06565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRO90143.1"
                     /translation="MTTSNNPADTWRPTTKNRLYDLVAPFDELLHKAVYWPKFNEWCR
                     TKAAGVPVFPDRLAQYQYVIDRYGLGGPIDFLEFGVFRGESMRWWLAHNTDPASRFIG
                     FDTFTGLPESWVGLPPGTFSADGKLPEFNDSRASLEIGLFQETLVGFLKRYPLERRTV
                     IHLDADLYSATLFVLSTLMPHIKPGDVVLFDEIGSAYGVTHEFRALCDIESSFGLRYR
                     VLAGADRFVRAAIEIQ"
     gene            9362..10819
                     /locus_tag="PLC06_06570"
     CDS             9362..10819
                     /locus_tag="PLC06_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRO90144.1"
                     /translation="MKNNAPPMNTARRLLGNTAFAFAANILTKAGTALLFIAIGRVLG
                     PAESGMFSLGTTWFTFVFGLSAFGLHEMMVREVAPRRAESAHYLANFLALRVSLSLVT
                     YALLLAGLWLVNPYSPDTTRVILIIALAAIPEGAFAIFQSLFEAHERLGILLVAAAVS
                     MAFKLGIGLWLLFSGASVVDLVWVVPAAGFISLLVFVPGLFRLFRHVPQTLPARLDSP
                     FVRRHLLPSFFVIHVLALLDYQVDALIISIMLGEESLGYYAAAQAILLAFNLMPAAIR
                     AALYPLMSRYHREAPDKLTILYDKVNKYIAALVLPLAAGLTLLAAPVIELIYGPDFTP
                     AAAVLQITIWAIVFLFLVEPHARMLLVYNKQRQAAWMMALTTALNIILNLLLIPAIGI
                     NGAAIARLISSLTLYLLFYAYCHFTIMPSKQPFSFRVILATLLMALAVLPLRDLFLLI
                     PIGVGIIVYLGAVRLLGVIPDQDLAYWREFYGKKA"
BASE COUNT         1917 a         3751 c         3250 g         2013 t
ORIGIN      
        1 ttcagcgacg gctatcagga ttagggctga cccgactctt gtccgccgcc gacggccaat
       61 ggacgacaga caccaaagcc tgtcgcccgt tggcctccat tgtggcgcaa gccgtccggc
      121 ttgcggatta gtcgaaacgc aacctcgaca gggtgcgcca cagttgcatc ttcaccctcc
      181 ctgagcaatt catctacatc tatgatataa ttgaacgatt aggaatttag ttatcccatg
      241 agttcagcaa accttccgcc ggcgtcgggc gcttattcgt cgcaacaacc atctgccgga
      301 aagccggggt cgtgggcggc atacgatcag ccaccgaacg agtggccgcc ctcgaacgcc
      361 ccctattcgt caacaaccta tcgccccacc cctgagcaat tgacccgcga cgagctgcgg
      421 cagcgttatc tgcgacgtaa cgtctacgcg ccgattatca tcgccgcgat cctcgccgtg
      481 gccttgttcg cgctgatcat ctatctggcg ttcggcgtgg caactcccca ggcgaaatcg
      541 ttcatcgccg gcatgtcggc gctcgtcgtc atcctgttcg ccgggccgat gatcatcctg
      601 atggccattc tgcccatcgc ctggctggcc ttcaggctca atcgtcgcca aaagcgcaag
      661 gagttcccgg agacggggcc gatggcctac cggagtcgca ttcaggcctg gttgtggcag
      721 ttggacgggc ttctcgacag cgccgaacat ggcgtggaca ggcttggcac gcgcatcaga
      781 caaccactga tcgccctgca cgcccgtttt gcttggctgc gtggttggct gaacggtatt
      841 agaggaaaat ttacacggag tatctagtat gagtaaatct gacagaacaa gaaatcagga
      901 gcttgccgcc atggaccagc aagaaagctg gcaggcgaaa gtcctgatca cgggcgggat
      961 catcggcgcg gccgtcggcc tggccacatc ctggctgctg attcgcacgt cccgcgagac
     1021 gcgcagcggc cccccggcca tcaccaccgg tgatgccatc aaggtcggcg tgacgaccat
     1081 tggcctggtg cgtgccattg ccgccctggg tgacaggcga gcgtagctca ggagcatatc
     1141 gcaatggcac aagaacaggg atcgtcccga cgcggccgat ccagaggggc ttccgcctca
     1201 tcacaaaaat caacaccgaa gcccaaacac gcttcccctc aatggcggcg gatggacctg
     1261 catctgcaca cgcccgcctc caacgaccac caggagccgc acgtcagcta cctggatatt
     1321 ttgcggcaag cggccgcccg cggcctcgac atcattgcca tcaccgacca caacaccgtg
     1381 gccggctttg ccaccatgca gaaagaagtc gagcaactat tatggctgga gagccgcggc
     1441 cgcatcgacc ccgccgagca acgcctgctg gatgagtacc ggcgcgtgct gggcaagctg
     1501 ctcgtcctgc ccggcttcga gttcaccgcc accttcggct ttcatattct gggcattttc
     1561 ccgccggaaa cgccggtgag ctatctggag catctgctgc tgacgctgcg cgtcccgccg
     1621 gcgtcgctgc gcgtgggcag ctcgaccgtc ggggccacgt ccgacgtgtt gaccgcctac
     1681 cacgtcatca atgaggccgg cggtctggtc atcgccgccc atgccaactc cggcaatggc
     1741 gtcatgctgc gcggtctcga tttcggcggc cagacgcgca tcgcctatac ccaggacgtc
     1801 aacctgcacg cactcgaagt gaccgacctg gagaagcgcg gcaaccagac gacccgccgc
     1861 ttcttcgacg gctccaagcc ggaatacccg cggccgatgc gctgcatcca gggttccgac
     1921 gcccaccgtc tcatccgcga atcggccaac tccccctatc tgggcgtcgg cgaccgcgtg
     1981 acggaaatat tgctggaaga agtgagcttc gacgcgctgg cgcgggtgat ccgcggcaac
     2041 gatcactcgc tgacgcgccc ctggcgcggc acggccaagg ccattgattt cgtccagtcc
     2101 gcccgcgaag agggcgagtc gctggtgcaa tctttccacc cgtccataaa ccagcgcggc
     2161 ggccatcagg acagcgtcct gcgcgacatt tgcgccatgg ccaacaccaa cggcggcacg
     2221 atctacgtcg gcattcccgc cgacccgaag gccaaacccg aaggggtgcg cgagccgcac
     2281 aaagccatcg agacgctgca gacacttatt tccaaacggt ttagcccgga gccgcaagtg
     2341 gtcatcgaca gcctgcccac gcaaggggcg accgtcatgc gcatcagcgt gacgcccggc
     2401 cccgacgcgc cctacgccat cgaccacaac cagttctacg tgcgcgacga gtccgagacg
     2461 accctggccg tgcgcgacga gatcgtccgg atggtggagc ggaacctgac cggtcgcgtg
     2521 gtggagcaaa agccgcccgc cgaaccggtc gccaacccga cgccaccgcc gtcatcgggc
     2581 cgcaagagcg agccgacgag ccgccccacc cctgccaacg gccgcggcgg ctatgaacac
     2641 ccccgcaccg gcgtggaaca cgtggacagc accgagcgcg acggcgtcat ctaccacacc
     2701 ctgcgcgacc tgcgcaacgg caacctgatc aacaacgtta cccgttcgtc ggcccgtcgc
     2761 ctgtggcatt acgccatcac ccaggtcgag actgctccgc ccgatctgtc ggtcgcgcaa
     2821 tggcgcaaca acgtcgccat tctcaatcgc cgccagaaga acaacaacac gctctatgac
     2881 ctggccgtgc gtgagggcga ccgtgcccac atctactacg gcgtgaccga gagcgggctg
     2941 agcgacgcgc tgttggctct cgtgaacgaa caggctcgcg aatgaggcgt gttggcatcc
     3001 tgaccatcag cgaccgcggg gccgagggcg tctacgagga caagagcggg ccgctggccg
     3061 ccgggctggt cgccgaacga accggctgga cgatcgctct tcagacgatc atccccgatg
     3121 agttcgagac gatcaccggc accttgcgcg aatggtgcgc cgccgggctg gacctgatcc
     3181 tgaccagcgg cggcaccggc tttgccccgc gcgacgtgac gcccgaggcg acccgcgcgg
     3241 tcatcgaacg cgaagcgccg ggcatcgccg aagcgctgcg ggccgagagt ctgaaggtaa
     3301 cgcgccatgc catgctctcg cggggggtgg ccggtctgcg cggcgccacg ctcatcgtca
     3361 acctgcccgg cagccccaag gccgtggccg agaacctcga tctgctgctg cccgtgttgc
     3421 cccacgcgct cgacctgctg accgggcgcg tcgagagcgg ccagggacac cgcgacgtat
     3481 aggggcggac atcgactccg ccttgatgat agtgagccat gaccagttcg ccacgcctgc
     3541 gcgccaacct gctcctgctg ctgacggccg tcgtctgggg cttcgccttc gtggctcagc
     3601 ggtcgggcat ggcgttcgtc ggcccgttca cgttcaacgc cgtccgcttc ctgatcggcg
     3661 ggctgatgct ggttcccctg atggtcgtca tggatcggcg aacgacggaa tcggggcggc
     3721 cgcccgtccc gctgcgcgat cgcaaccttc tcacgggtgg cgtgctggtc gggctggtcc
     3781 tgtttggcgc ggccacgctg caacagatgg gcgtggccct caccacggcg ggcaaggcgg
     3841 gcttcatcac cagcctctac gtggtgctcg tgccgctgct ggggctggcg ctgggtcatc
     3901 gctcctcggc cgcggtgtgg cttggggcga tcctggccgc ggcgggcctc tacttcctga
     3961 ccatcgaagg cggcttctat atggcctggg gcgacctgct ggtgctgttc ggcgcgtttc
     4021 tgtgggccgg gcacgtgttg ctgctcggcc gcctctcgcc ccaaaccgat acggtcaagc
     4081 tggccttcct gcaatttgtg gtctgcgccg cgctgagcgc cgcggccgcc ttgctgaccg
     4141 agacgatctc ggccgccgac ctgcgcgccg cgctgtggcc gattctctac acgggcatca
     4201 tgtcggtggg cgtgggctac acgctgcaaa ccatcgccca ggcccacgcg cgcccggccg
     4261 acacggccat catcctcagc ctggaagcgg tcttcgccgt catcggcggc tggctgctgc
     4321 tgggcgaggt gctgtcggcg cgtgccctgt tcggctgcgc cctcatgctg gccggcattc
     4381 tcatctcgca gctgctcggt cgtccggaag acgcggcggc cgagattagc cattgacaca
     4441 cgacgagtga caaatgacga gcacggaaca acccacagcc accctcatcc tgaagccggg
     4501 ccgcgacaag ccggtccaca atcgccatcc ctgggtcttt tcgggggcca tcggccgcgt
     4561 caagggccaa cccgcaccgg gcgacctggt cacggtggcc gaccacaagg gcacgccact
     4621 ggccaccgcc tattacaatg ccaaatccca aatccaggcc cgcatcctct cctgggaacc
     4681 ggccgtccca atcgacgaat ccttctggcg caaccgcctc caacaagcca tccacggccg
     4741 ctccctgatc accaccccac tgattactga ttcactgaat actgacccac tgatcactgt
     4801 cccactgccc accgcccccc tgaacaccgc ccatcgcctc attaacgccg aagccgatct
     4861 cctccccggc ctcgtcgtcg accgctacgg cgattacctc gtcatgcagt gcctcaccct
     4921 gggcatcgac ggccgcaagg agatgctggc ccgcctattg gcagagctgc tccacccggc
     4981 gggcatcctg gagcgctcgg atgtggacgt gcgcggcaag gaagggctgc ggccgttggt
     5041 cgagctgcgc cacggacagg agccgccggc cgaactgacc atccgcgaaa atggcttctc
     5101 cttcctcgtc gatctgcgcc acggccacaa gacaggcttc tacctcgacc agcgcgacaa
     5161 ccgggccacg gccgggcaac ccgcgctcat ggccgggcgc gaggtgctca acgtcttcgc
     5221 cttcaccggc gcgttcggcg tctatgccgc ggccgccgga gcgcgtaatg tcgtccagat
     5281 cgacagctca gcccctgcgc tggaaacggc cgaacgaaat atgaagctca acggactgga
     5341 taaagcatcg gacgaataca tcgccgggga cgctttcgag gtgttgcgct acttccgtga
     5401 ggaagggcgg caattcgacg ctatcatcct cgatccgccc aagtttgccc acagccagag
     5461 ccagatcgac cgcgccgcgc ggggctacaa agacctcaac tggctggcga tgcgcctgct
     5521 gcggcccaac ggggtgctgg cgaccttctc ctgctccggt ctggtcagcg ccgacctgtt
     5581 tcagaagatc gtcttcggcg cggccgtcga tgccgggcgc gacgcgcaaa tcctgcatca
     5641 cctgggccag tcggccgacc atcccgtcct gctttccttc cctgagtcgg cctacctgaa
     5701 gggcctgctc tgccgggtga tctgatgcgc gtctcggtca tcatccccag cctgaacgcg
     5761 ccgacgctgg gtcgggcgct ggccgccgtc gtcgcccaat cggagccgcc gcacgaggtc
     5821 atcgtcgtcg gccgcgacga ggcgggcacg ttacagacac accccgccgt acagttcatc
     5881 gacaccggcg tgccggtctg tgccgcgcgg gcgcgcaatc tgggcatggg cgccgcgtcg
     5941 ggcgacctgt tcctgctcct cgactccgac tgcatccccg cgcccgactg gctagcccgc
     6001 caccgcgccc gccacgccgc cggagagacg gtcgtcggcg gggccgtggc gctacagggt
     6061 agcaattact gggcgcaaag cgataatgtg tccatgttcc acgatttcgt cccccagctt
     6121 ccggccgggc cgcgctttct gctgcccacg ctcaatttga gcgtccgccg cgaggtgttc
     6181 gccgccgtgg gcggcatgga tgaatctttc cccggcgcag cggccgaaga tgccgactgg
     6241 acggtgcgaa tccgtcgcgc gggccatcga ctctggttcg atcctgcggc cgtcgtcgcc
     6301 cacagcccgg cccgcgtcgc gccgcgtgac gtcgtgcgcc attggcgcta cctgggccac
     6361 aacgccatcc gtgtgcgcct gcgctatgcc gacgaattta aaacgcccaa tggggcgcaa
     6421 tacgcctggt ggtggcgtct gttttcgccc atcatcgccg ccggcgtgac cgccggaatc
     6481 tacgccaagc ggccgctccg gcgctattgg gccggcctgc cggtcgttta tgcgaccaag
     6541 atcatctact gctggggagc ggcccgcgcc atcgacagcg gctttgccac acaaccctga
     6601 tatgccgccc gtcaccgtcc tcatcgtcgc ctggaatcag ctcgccaaga cgctggattg
     6661 tctggcgacg gtggccgcgc tcgattaccc cgactttcgc gtcctgctgg tcgataacgg
     6721 cagcgagccg ccgctggccg aggcaatcgc ggcccgcttc cccgacgtcg ccacgctccg
     6781 gctgcccggc aatctgggct ttgccggtgg ctataacgcc gggttacgcc aagggttagc
     6841 aggcgacggc cgctattttc tgctgctcaa caacgacacg ctgctggagc cggacgtttt
     6901 gtcgaagctg gtggccgaga tcgagcgcgc ggccggcatc gggctggtga cggccaaaat
     6961 ctattacgcc gccgaccggc aacgcatctg gactgtgggc gccaacctca acgtcttcct
     7021 cgatctaaag gaaggcggcg ggggccagat tgacacggga caatgggaca gcccgcgcga
     7081 catcgatttc gccccttttt gcgcgctgct catccggcgc gaagtgcttg aggaagtggg
     7141 gctgctggat gaggggttct ttctctatta tgaagatatg gactattgtc gccgggcgaa
     7201 ggcggctggc cggcgcgtgc ggctttgccc caccgctcat gtgtaccatg acgtcagcgc
     7261 cagcagcggc ggccgcgaca gcccgatgga acgctactgg atggcccaga gcagcggccg
     7321 atattttcgc aaacacggcc gcggcctgcg cctgctgctc atcatcccgt tccggctggc
     7381 cagcgcgatc aagatgaccg cacggctatt ggtgcagggc aaacatgaag cgttgcgcgc
     7441 ttattggtca gggctgataa tcggctggcg caccggccgg gccaccacgc cgccaaaggc
     7501 gaagcgcgcc atcgaatcca cctgataacc cattcgcgga agagcttcca tgacctacaa
     7561 acggcaaatg cgccaactgg cgaaacgaat catccccaaa tcccccctcg tcaacacctg
     7621 ggccgcctgg tgtcgcctga aggctcgcca tgcgcggcgc atcttcgaag aggccggcga
     7681 ctcacctgcc tggctgggga cggccgaact cgacgcgcta caacgcgctt acccgcttga
     7741 gccactctat tatggctatg accctgaaag catcgtcgcc cgcggccgcg aacgggcgca
     7801 agccatgctc aacctcgtcc accactccga ccggttcagc cgcttcctcg atctgggcat
     7861 gtgggacggg atgacctgct acgcgctgcg cgagctgggc gtggcgacga cgggcatcga
     7921 cattcgcgtc gaagggctgg accagcgcgc cgtcgatagc ggcgcccact tcgccgccat
     7981 ggacgcggcg catctgggtt tcgccgccga gagcttcgac ttcgtgttct cgttcaactc
     8041 cttcgagcac ttccccgacc cggagctggt attgcgcgag acgttgcggg tgttgcggcc
     8101 gggcggctat ctctacatcg acttcggccc attgtggctc acgcccaagg gggcgcacca
     8161 gttcgagacc atcaccgtgc cctatgtcga atgtctgttc tcgaaagaaa cgctcaccgc
     8221 ctatgcgacc gccaacgaca tcccgctaaa gccctacgaa tggatgaatg aatggccgct
     8281 gtcgcgctat cggagcttgt ggcagcgcta tgaaggccaa atggagcgcg tgacttacca
     8341 tgagatctac aatgccgatc atgtcgatct gatcgcccgt taccccacct gcttcaagag
     8401 caaaacccgg acgttcgacg acctgatcat ctccaacatc gaggctgtat ttcgtaagcg
     8461 gtaaggaata gccgaggggt tattggatct cgatggccgc gcgcacgaac cgatccgcgc
     8521 cggccagcac ccgatagcgc aagccgaaag acgattcaat atcgcacagg gcgcgaaatt
     8581 cgtgcgtgac gccataggcc gaaccgattt catcaaagag gacgacgtcg cccggcttga
     8641 tgtgcggcat caaggtgctg agcacgaaga gggtggcgct gtagagatcg gcgtcaagat
     8701 ggatgacggt gcgtcgctcc agcgggtagc gcttcaggaa gccaacgagc gtttcctgga
     8761 acaggccaat ctccagcgag gcgcggctat cgttgaactc cggcaatttg ccgtcggccg
     8821 agaaggtgcc cggcggcaaa cccacccacg actccggcag cccggtgaag gtatcgaagc
     8881 cgatgaagcg cgaagccgga tcggtgttat gggccagcca ccaacgcatg gattcgccgc
     8941 gaaacacgcc gaattcgagg aaatcgatcg gcccgcccaa gccgtagcgg tcgatcacat
     9001 actgatattg ggccaatcgg tcggggaaca cgggcacgcc cgcggccttt gtgcggcacc
     9061 attcgttgaa cttcggccaa tagaccgcct tgtggagtag ctcgtcgaaa ggagccacta
     9121 aatcatagag gcggttcttg gtggtcggcc gccatgtgtc tgcgggatta ttgctggtcg
     9181 tcatacagga atagttatat ccgtcgttaa tttcgcaggc gcaatcatag catgataggt
     9241 atttgacgcc aatccacccc cgcccccttt ctcccccttc tccctttctc gcatgtggca
     9301 gtcgccccgc ccgcttgcta caatcagcgc ttgtcgttcg ctggccgccg gccttcgatt
     9361 gatgaagaat aatgccccgc ccatgaacac cgcccgacgc cttctcggca acaccgcgtt
     9421 cgcttttgcc gccaacatcc tgaccaaggc gggcaccgcg ctgctcttca tcgccatcgg
     9481 ccgcgtgctg gggccagccg agtcgggcat gttcagcctg ggcacgacct ggttcacctt
     9541 cgtcttcggg ctgtcggcct tcggcctgca tgagatgatg gtgcgggaag tcgctccccg
     9601 gcgcgcggag agcgcccatt atctggctaa cttcctcgcg ttgcgcgttt ccctgtcgct
     9661 tgtcacctat gctcttctgc tggcgggtct gtggctggtc aacccctatt cgcccgacac
     9721 cacccgggtc atcctgatca tcgctctggc ggcgataccc gaaggggctt tcgccatctt
     9781 ccagtcgctc tttgaggccc acgagcggct gggaatcctg ctcgtggccg cagcggtcag
     9841 catggcgttc aaattgggca tcgggctatg gctgctgttt tccggcgcgt cggttgtcga
     9901 tttggtgtgg gttgtgcctg ccgccgggtt catcagcctg ctcgtgtttg ttcccggcct
     9961 gttccggctg tttcgccacg tcccccagac cctgcctgcc cggcttgact cgccgttcgt
    10021 gcgtcgccat ctgctgccca gcttcttcgt catccacgtc ctggccctgc tcgactatca
    10081 ggttgacgcc ctcatcatct cgatcatgct gggcgaggag tcattgggtt attatgccgc
    10141 ggcgcaggcg atcctgctgg cgttcaacct catgcccgcc gccatccggg ccgcgctcta
    10201 cccgttgatg agccgctacc atcgggaagc gcccgacaaa ctgaccatcc tctacgacaa
    10261 ggtgaacaag tacatcgccg cgctggtgct gcccctggcc gccggtctca ccctgctggc
    10321 cgcgccggtc atcgagctga tctatggccc cgatttcacg ccggcggcgg ccgtcctgca
    10381 aatcaccatc tgggccatcg tcttcctgtt tctcgtggag ccgcacgccc gaatgctgct
    10441 cgtctacaac aagcagcgtc aggcagcgtg gatgatggcc ctaacgaccg cgctcaacat
    10501 catcctgaac ctgctgctca tccccgccat cggcatcaat ggcgcggcaa tcgcccgtct
    10561 catctccagc ctgaccctct acctgctgtt ttacgcctat tgccacttta ccatcatgcc
    10621 ctcgaagcag cccttctcct tccgggtcat cctggccacc ctgctcatgg ccctggccgt
    10681 gctgccgctg cgcgatcttt tcctgctcat ccccatcggc gtcggcatca tcgtctatct
    10741 gggcgcagtc cggctgctgg gggtcattcc cgaccaggat ttggcttatt ggcgcgaatt
    10801 ttacggcaaa aaggcctgaa tcgcggaaat tactccccta aatactccaa aaggatgatt
    10861 gatttacctc ccgcgggcac ctatacttcc cattgtaaac tgctaaccat agcgaacttg
    10921 tgtgaggaga t
//
LOCUS       DAOMQG010000083        10854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2602_length_10854_cov_10.638670, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000083 DAOMQG010000000
VERSION     DAOMQG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10854
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_10854_cov_10.638670"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLC06_06575"
     CDS             <1..552
                     /locus_tag="PLC06_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HRO90145.1"
                     /translation="SQSGRKALYLHEGRAEDDQAKGDASMNREELLKNIHAGRGELRS
                     AFGRYNKHDMIEPVLPNGWSVKDVIAHIGFWERRVVNLYNTLADGSVPDDPIDSLGLD
                     ELNARILEDNQLVPLGIAQVNELEAYQELLHLVETAPEGDLFDPQRFAWTEGEPFYNW
                     IIGNSYGHYADHIAELLEGAEQL"
     gene            627..697
                     /locus_tag="PLC06_06580"
     tRNA            627..697
                     /locus_tag="PLC06_06580"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:659..661,aa:Gln,seq:ctg)
     gene            787..>2360
                     /locus_tag="PLC06_06585"
     CDS             787..>2360
                     /locus_tag="PLC06_06585"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRO90146.1"
                     /translation="MNIRPARPEDVPGILFLVNEHARRGDLLPRTAASIRDTLDDWLI
                     AKDETGEIVACVSLFAYSPVLAEVRSLAVHDRAKGGGWGSTLVKALILEARHRNVETL
                     FALTRAVRFFERAGFHITNRERFPEKVWRDCSICPLIDNCDETAVVLNLAHPLGPMEH
                     SPSASDRRPTPLAIEHQAPIKKPHHLSAGEKSMSKPVVEKVVLAYSGGLDTSVIVPWL
                     RENYGCEVICFCANIGQGDHEFDGLEEKGLASGASKVIVEDLRHEFAKDFLIPMMQAG
                     AVYEHQYLLGTSVARPLIAKWQVAVAEAEGADAVAHGCTGKGNDQVRFELTYKALNPT
                     LKVIAPWREWNIRSREDALAYARAHNVPVTQTEKSIYSRDANMWHLSHEGGILEDPAN
                     TPEEAMFQLTVSPENAPDEPEIVEITFERGMPVAVNGVQLPPAAVIETLNKLGSKHGI
                     GRIDLVENRLVGMKSRGVYETPGGTILYAAHRELESLCLDRETMHFKQQMALRYAELT
                     YFGMWYHTLRESLQAFV"
     assembly_gap    2361..2460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2461..3597
                     /locus_tag="PLC06_06590"
     CDS             <2461..3597
                     /locus_tag="PLC06_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO90147.1"
                     /translation="LGMPDSLLGIAWPTMRVDFGVPLDMLGTLLIGSVSGYMISSFMS
                     GALVGRYGVGKILAASCFLTGLALAGYTIVPEWWMMVLLGVVAGLGAGAIDAGLNTYA
                     AAHFSEGLVQWLHASYGIGITMGPIIMTMALTTYDSWRVGYRIVAGFQWAMAIAFVLT
                     LSWWGDQKSSDGEKRLTDYKTPMAETLRRPRVWLSVLLFFLYVGAEVSLGTWGFSLLT
                     ESRGMETALAGFLIGSYWATFTIGRVLAGLFAKRLGVNVLVGGGIILALLGALLLWWN
                     PFESANVLAVVIIGLAVAPVYPGLISGTSSRVDAKYAANTIGMQMAASGLGSALIPGM
                     VGVLAQRLSLEAIPIFLAATFMTLLILYRLSMTARRQTEPIDTH"
     gene            3786..5012
                     /gene="argJ"
                     /locus_tag="PLC06_06595"
     CDS             3786..5012
                     /gene="argJ"
                     /locus_tag="PLC06_06595"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRO90148.1"
                     /translation="MTHQTLHEGGVTSPQGFTAAAVAAGIKQSGKPDLVLVISDRDCS
                     AAGIFTRNQVAAAPILVDRETLAANSTAIRGVVINAGNANACTGEPGLANAREMQRLA
                     AGAVGGSAGQYLVMSTGVIGIPLPMDRVGAGIDAAASRLSSANSRAAAEAIMTTDTHP
                     KFMALEVDLPGGRVILGGMAKGAGMIHPDMATMLCVLTTDAAIPAAMLDELLREAADR
                     SFNAISIDGDTSTNDTVLLLANGASGVTIDDEASRVVFSTALGELCHALAMMIVRDGE
                     GVSRVVTVQVTGAATAADARKIANTIATSPLVKTAFAGGDPNWGRIMMATGRSGVELR
                     QDRLALWIAAPDGDPLAIVRNGTPTDYRESDAAAVFALPEFIVHVDLGAGTGEATIWT
                     TDLTHDYVTINADYRT"
     gene            5049..6422
                     /gene="argH"
                     /locus_tag="PLC06_06600"
     CDS             5049..6422
                     /gene="argH"
                     /locus_tag="PLC06_06600"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRO90149.1"
                     /translation="MMSKLWGGRFTGQTNSLVHRYNASISFDARLYDEDITGSIAWAE
                     ALAAAGLLTSQEQEALSNGLEAVRLEFAEGTFVIQPEDEDIHSAVERRLTELAGPVGG
                     KLHTGRSRNDQVATDFRLWVMRACAILDAHLARLQASLADSAELALNLPMPGYTHLQH
                     AQPVTWGHWILSHFWPLVRDRERLEAVRHAAAVLPLGAAALAGTAFPIDRESLAKRLG
                     FHSITPNSLDAVSNRDFAAEFLFAAALIGVHLSRLSEQLILFSSAEFGFISLDDAYST
                     GSSIMPQKKNPDTLELTRGKSGRLIGHLTGFLATLKGLPSSYDKDLQEDKEPVFDAFD
                     TLDFALPVMAGLIGTLELRPERMAAQLEAGLLATELADYLVRRGLPFREAHHAVGQAV
                     RLAEERRMTLTDLTAADLQAISLHFGEDAPAVLSVPAALAARGVPGGTAPRALAEQLR
                     LAREQIG"
     gene            complement(6424..7257)
                     /locus_tag="PLC06_06605"
     CDS             complement(6424..7257)
                     /locus_tag="PLC06_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HRO90150.1"
                     /translation="MEQSTSVNKTAVADKTKTRSVSFKSVALPAEHGSWSLVSEPIVL
                     GMLVAPTWAGLALVIAAFFIFLFNRPFKVYWTDRRRGRFYERTAMARRYALIFGAVAL
                     VAAIAAFALGGWRPFVPFLLAAPLLLVFMIYDQRPGRHWQAELAAPVAFAALVMSVAL
                     AGGYSWVVGLALWGFMSLRAVPAVMFVRARIKLDKGKPVSVWGTIGAHIAAVVLVSLF
                     VWLGWLPMTAAIAMAILLGRAIWGFSNYRWRSSMKALGFLETGFGLLAVFLVAIGFWL
                     Q"
     gene            complement(7261..7560)
                     /locus_tag="PLC06_06610"
     CDS             complement(7261..7560)
                     /locus_tag="PLC06_06610"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HRO90151.1"
                     /translation="MLREVLQAVEAAEGPVTLTELSRQLNLDPRVLDGMLEHWSRRGK
                     LVVNSETAMACAGGLKEPGHCSCGSSASGDGCPFIARLPKSYAVFTPTEFDVELR"
     gene            complement(7560..9791)
                     /gene="feoB"
                     /locus_tag="PLC06_06615"
     CDS             complement(7560..9791)
                     /gene="feoB"
                     /locus_tag="PLC06_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRO90152.1"
                     /translation="MTPHLLSTESNVLSVALAGNPNAGKSTLFNALTGARQHVGNWPG
                     KTVEQKTGEFARHGRRIAITDLPGTYSLSAYTMEELVSRDYLLDSRPDAIIAVADATN
                     LERNLYLIVQLLELELPTVVALNMMDVAAKQGLQIDIPELSCSLNVPVIPSIARQERG
                     IDEIVAAVSHASTLPTSACSLDYGEAIERAIASLLVIINDYIAIREYYAPRWLAVRLL
                     EGDVDVAARLSGLPGGSELLPAAGKRRAELAAALGEDIDLFIADRRYTWIHNLAGRVV
                     RAGSAHTMSNSDRIDHVMTDRRLGIPIFMLAMWLVFKITTDLSAPWVDWIDGVLSGPI
                     SRWIMTVLDAIGLGETWVASLLIDGVIAGVGGVLVFVPVLMALYFALAVLEDSGYMAR
                     GAFVMDRLMNRIGLHGRSFLPLMVGFGCSVPAIYATRTLTNERDRIMTGLLVPFMSCG
                     ARLPVYVLFAAIFFPRYASVVIMGLYLLGVIIAIGVGLLLQHSLLPAAAAPGLVMELP
                     PYRLPTARGIGVQMWIRTRAFLQHAASLILVTTIAIWFLTAIPAGAHGTFANVAMADS
                     VFGRASNAMASVLEPLGFGTWESGGALISGLVAKEVVVSTLAQTYGTSGAIDEARSDT
                     FLQDLGEIVTSFLGAILDTLKAIPGLIGINLSVGDDEAVPGLADAIRASFTESSGGHA
                     AAAGLAFMVFVLLYTPCMATIAAMRHELGTRWMWLSFVGQLAIAWLAAFFVFRLGVWV
                     GGL"
     gene            complement(9815..10204)
                     /locus_tag="PLC06_06620"
     CDS             complement(9815..10204)
                     /locus_tag="PLC06_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRO90153.1"
                     /translation="MTGPVTLWIVVAILALATVMFVVRRVSTPRPLPVMDERVAGLTA
                     AGFLPLVDCPRGRDACLHCLLVDHCVVHRLLELGLTPGTRVRVVQDAGGPMLLSVRGS
                     RVALGRDLAARVWVELPDRESGKVSCG"
     gene            complement(10204..>10854)
                     /locus_tag="PLC06_06625"
     CDS             complement(10204..>10854)
                     /locus_tag="PLC06_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO90154.1"
                     /translation="TWLTLSEAAELLNVHPTTLRRWSDSGDIPTLVTPGGHRRFAATD
                     LARFARERSALRNVNSLTGLWATKALAYTRQETMADRKEGWLTYFDEEARNQNRKLGQ
                     QLMGLTLQYLSSESSEEAGASILEEAGRIGRLYAENARNQGLPLRATLEASIFFRDSL
                     IETALQLPESANIRPEANMRLLRRVNDLLNTVHLAVADVYDQEVSERASTQLGDAL"
BASE COUNT         2327 a         3318 c         3146 g         1963 t
ORIGIN      
        1 tcacaatccg gcagaaaagc gttatacttg catgaaggtc gtgctgaaga tgatcaagcc
       61 aaaggagacg caagcatgaa ccgcgaagag ttgctgaaga acatccacgc aggtcgtggg
      121 gaacttcgta gtgcgttcgg ccgctacaat aaacacgata tgatcgagcc ggtgctgccg
      181 aacggctggt ccgttaagga tgtcatcgcc catatcggtt tttgggagag gcgtgtggtg
      241 aatctctaca atactctggc cgatgggagc gtgcctgacg atccgattga tagcctgggg
      301 cttgatgaac tgaatgcgcg tatcctggaa gacaaccagc tcgtgcctct ggggatagcc
      361 caagttaatg agcttgaagc ttaccaggag ctgttacatc tcgtcgaaac cgcgcccgaa
      421 ggagacctgt tcgatccaca acggttcgcc tggaccgaag gggaaccgtt ctacaactgg
      481 atcatcggca attcctacgg gcattacgcc gatcacatcg ccgaattgct ggaaggcgcc
      541 gaacaactat aggctcctct gcatgtcgcc gttcgggcat tgatttcttg cccacccatc
      601 gtccgaatgg tataatgcgg ccacattgag ggatagttta acggcagaac aacggactct
      661 gactccgtga gtcgtggttc gaatccacgt ccctcagcta accggaaggc cactcaagag
      721 agtggctttt ttcgtagggt atcgttacat tttagctata acttacttat tattgaccgg
      781 agtcacttga atatccgacc cgcccggcct gaagacgtac cgggcatcct ttttttggta
      841 aacgaacatg cgcgccgcgg agatctatta ccgcgaacag ccgcgtcgat ccgcgacacc
      901 ctcgacgatt ggctcatcgc caaggatgag accggcgaga tcgtcgcctg tgtatcgttg
      961 tttgcctatt cgccggtatt ggccgaggtg cggtctctgg ccgtccatga ccgggccaaa
     1021 ggcggcgggt ggggcagcac gctcgttaag gcgctcattt tggaagcccg tcaccgaaac
     1081 gtcgagacgt tgttcgccct gactcgcgcc gtccgcttct tcgaacgagc cggattccat
     1141 atcaccaatc gcgaacgctt ccctgaaaag gtgtggcgcg attgcagcat ctgccccctg
     1201 atcgacaatt gtgacgaaac ggccgtcgtg ctgaatctgg cccacccact tggcccgatg
     1261 gaacattccc cttccgcaag cgaccggcga cccacacctc tcgctatcga gcatcaagcc
     1321 cccatcaaaa aaccacatca tttatctgca ggagaaaaat caatgtccaa accggttgta
     1381 gagaaagtcg tccttgccta ttccgggggc ctcgacacct cggttatcgt cccctggctg
     1441 cgcgagaatt atggctgcga agtcatctgt ttctgcgcca atatcgggca aggcgaccat
     1501 gaattcgatg ggctggaaga gaaagggttg gccagcggcg ccagcaaggt gatcgtcgag
     1561 gatttgcgcc acgaattcgc caaagatttt ctcatcccga tgatgcaagc cggcgctgtc
     1621 tatgagcacc agtatttgct cggcacttcc gtcgcccgac cgttgattgc caagtggcag
     1681 gtggccgtgg ccgaggccga gggcgctgat gctgtggctc atggctgcac cggcaagggc
     1741 aatgatcagg tacgcttcga attgacctac aaagcgctca acccaacact gaaggtgatc
     1801 gccccctggc gcgagtggaa catccgctcg cgtgaagacg ccctggccta tgcccgcgcc
     1861 cataacgtgc cggtgacgca aaccgagaag tcgatctaca gccgcgacgc gaacatgtgg
     1921 cacctctccc acgagggcgg catcctcgaa gacccggcca acacgcccga agaagcgatg
     1981 ttccagttga cggtgtcgcc ggagaacgcg cccgacgagc cggaaatcgt cgagatcacg
     2041 tttgaacggg gcatgccggt agcggtcaac ggcgttcagt taccgccggc cgcggtcatc
     2101 gaaactttga acaagttggg cagcaagcac ggcatcggcc gcatcgattt ggtagagaac
     2161 cgcctggtcg ggatgaaatc gcgcggcgtc tacgagacgc ccggcggcac cattctgtat
     2221 gccgctcacc gcgaactgga atcgctctgc ctggatcggg agaccatgca cttcaagcag
     2281 cagatggcgc tgcgttatgc cgaactgacc tacttcggca tgtggtatca caccctgagg
     2341 gaatcgctgc aagcctttgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 ctgggcatgc ccgacagctt gttgggcatt gcctggccca ccatgcgcgt ggatttcggc
     2521 gttccgctcg acatgctggg cacgctcctg ataggcagcg tatccggtta catgatctcc
     2581 agcttcatga gcggggcgct ggtggggcgg tatggggtcg gcaagattct ggccgccagt
     2641 tgttttctga ccggtctcgc tcttgcgggc tacaccatcg ttcccgaatg gtggatgatg
     2701 gttctgttgg gcgtggtggc cgggttaggg gcgggcgcga tcgatgccgg gctgaacacc
     2761 tatgccgccg cccacttcag tgaaggattg gtccaatggc tacatgccag ctatggcatc
     2821 ggcatcacga tggggccgat catcatgacc atggccctga ccacctatga ttcatggcga
     2881 gtcggctatc gcatcgtcgc cggtttccaa tgggcaatgg caatcgcttt tgtgctcacc
     2941 ttgtcctggt ggggcgacca aaagagcagc gacggggaaa aacggcttac ggactacaaa
     3001 acccccatgg cggagacctt gcggcggccc agggtctggt tgagcgtgtt gctgttcttc
     3061 ctctacgtcg gcgctgaggt ctcgctggga acgtgggggt tcagccttct gactgaatcg
     3121 cgcggcatgg aaaccgcgct ggccgggttc ctgatcggca gttattgggc aaccttcacg
     3181 atcggccgcg tcctggcggg cctcttcgct aaacgcctgg gggtaaacgt ccttgtcggg
     3241 ggtgggataa ttctcgccct gttaggcgcg ctcttgctct ggtggaatcc gtttgaatca
     3301 gcgaatgtgc tggcagtagt gataataggc ctggctgtcg cgccggtcta ccccggcttg
     3361 atctccggca ccagcagccg agtcgatgcg aaatatgccg ccaacaccat cggcatgcag
     3421 atggcggcca gcgggctggg gtcggccctc atccccggca tggtgggggt gctggcccaa
     3481 cgcctgtcgc tggaagccat ccccattttt ctggcagcga ccttcatgac cttgttgata
     3541 ctatatcggc tatcaatgac cgcacgacga cagacagaac ccattgatac tcattaacgg
     3601 tcaacctgaa cccacgccgt tggcaaacca atgaagacga tgctacgatc atcgcattgg
     3661 cgcggccaga acctgcccgg ttcatttacg ctcgaatgat cataaggagt gcccgcgcct
     3721 ttcccggccc tggccaattt agtgaacaag gcccaaacac gcgggacgga atattaaacg
     3781 aaaagatgac acatcagacg ttacacgagg gtggggtcac cagcccccaa ggcttcaccg
     3841 ccgcggccgt ggccgccgga atcaaacagt cgggcaagcc cgatctggta ctggttatat
     3901 cggaccgcga ctgctcggcc gcgggcattt tcactcgcaa ccaggtggcc gccgcgccga
     3961 tcctggtcga ccgggagacg ctggccgcca actccaccgc gattcgcggc gtggtcatca
     4021 acgccggcaa cgccaacgcc tgcacgggcg agccaggtct ggccaacgcg cgcgagatgc
     4081 agcgtctcgc ggcgggtgct gtcggcggaa gcgccgggca atatctcgtg atgtccactg
     4141 gcgtgatcgg catcccgttg ccgatggatc gcgtgggagc ggggatcgat gccgccgctt
     4201 cgcgcctgtc atccgccaac agccgcgcgg cggccgaggc gatcatgacc accgacaccc
     4261 atccgaaatt tatggccctt gaggtcgatc tgcccggtgg tcgggtcata ctgggcggga
     4321 tggcgaaagg cgcggggatg atccaccccg atatggcgac gatgctgtgc gtgctgacaa
     4381 ccgacgccgc catcccggcc gccatgctgg acgagctcct gcgagaggcg gccgatcgca
     4441 gcttcaacgc catctccatc gacggtgaca cgagcaccaa cgacaccgtc ctgctcctgg
     4501 caaacggcgc gagcggcgtg acgatcgacg atgaggccag ccgcgtcgtc ttcagcacgg
     4561 cgctcggcga actctgtcac gcgctggcga tgatgatcgt gcgggacggc gagggcgtat
     4621 cccgcgtcgt cacggtgcag gtcaccggcg cggccacagc tgccgatgcg cgaaagatag
     4681 ccaacactat cgccacttca cccttggtga aaacggcgtt tgccggcggc gacccaaatt
     4741 ggggccgaat catgatggcc acagggcgca gcggcgtcga attgcggcaa gatcgcctgg
     4801 cgttatggat tgccgcgccc gacggtgacc cgctggccat cgtgcgcaac ggcacgccga
     4861 ccgattatcg cgagagcgac gccgcggccg tcttcgccct gccggagttc atcgtccatg
     4921 tcgatctggg cgccggaaca ggagaagcca cgatctggac gaccgacctg acccacgact
     4981 atgtgacgat caacgcggac tatcgcactt gatgccgggc cggcgcttga tcattccgac
     5041 aacgagatat tatgagcaag ctatggggcg gtcgattcac cggccagacc aactcactgg
     5101 ttcatcgcta taatgccagc atcagcttcg acgcgcggct atatgatgaa gatataaccg
     5161 gcagcattgc ctgggccgag gctctggccg cagcagggtt gctgacctcg caagaacagg
     5221 aggcgctcag caacggtctg gaagcggtgc gacttgagtt cgccgaaggg acattcgtca
     5281 tccagccgga ggacgaagac attcattcgg ccgtcgagcg gcgcctcacc gaactcgcag
     5341 ggccggttgg cggcaagctc cacaccggcc gcagccggaa cgatcaggtt gccacagact
     5401 ttcgcctgtg ggtcatgcgc gcctgcgcga tcctcgacgc ccacctcgcc aggctgcaag
     5461 cgagcctggc tgactccgct gaattagcgc tcaatctgcc catgccgggc tacacccatt
     5521 tgcaacacgc gcagcctgtc acatgggggc attggatcct ctcccatttc tggccgcttg
     5581 ttcgcgatcg cgagcgactg gaggccgtgc gtcatgccgc ggccgttctg cctctgggcg
     5641 cggcggcgct ggccggcacc gcgtttccaa ttgatcgcga gagcctggcg aagcgtctgg
     5701 gctttcattc catcactccc aacagtctgg atgcggtcag caaccgggat ttcgcggccg
     5761 aatttctttt cgccgctgct ctgatcgggg ttcacctcag ccgcctgtcg gagcagctaa
     5821 tcctgtttag cagcgcggag ttcggcttta tcagcctgga tgacgcctat agcaccggct
     5881 ccagcatcat gccacagaag aagaaccccg acacgctgga actcacgcgg ggcaagagcg
     5941 gccgcctcat cggccatctg accgggtttc tggcgacgct taagggtttg ccgtcaagct
     6001 acgacaagga tctgcaggaa gacaaggagc cggtattcga cgcgttcgac acgcttgact
     6061 tcgccctgcc ggtcatggcc gggctgatcg gcacgctgga gctacggccg gagcgcatgg
     6121 cggcccaact ggaagcgggg ttactggcga cggaactggc cgattatctg gtacgtcgcg
     6181 gcctgccctt ccgcgaggcg catcatgccg tgggccaggc cgtgagattg gccgaagaga
     6241 gaaggatgac gctcaccgac ctgaccgccg ccgaccttca ggctatttct ctccacttcg
     6301 gcgaggatgc cccagcggta ctgagcgtac ctgccgccct ggccgcgcgc ggcgttcccg
     6361 gcggcaccgc accccgagcg ctggccgaac aattacgatt ggctcgagaa cagatcggct
     6421 aatttactgg agccagaaac cgatagccac caggaagacg gccagcaggc caaagccggt
     6481 ttccagaaag cccagcgctt tcattgaaga acgccagcga taattggaga agccccatat
     6541 cgcccgcccg aggaggatgg ccatcgcgat ggcggccgtc atcggcagcc accccagcca
     6601 aacgaagagg ctaacaagca caacggccgc gatgtgtgcg ccgattgtcc cccacacaga
     6661 tacgggtttg cccttgtcga gttttattcg cgcgcgaacg aacatgacgg ccggcacggc
     6721 gcggaggctc atgaatcccc acaaggccaa cccgacaacc catgaatagc cgccggccag
     6781 agcgaccgac atcaccagcg cggcaaacgc gaccggcgca gccagctccg cctgccaatg
     6841 gcggcccggc cgctgatcat agatcatgaa gacgagcagg agcggcgcgg ccaggaggaa
     6901 aggcacgaac ggccgccatc cccccagagc aaaggcagca atcgccgcca ccaaagcgac
     6961 cgccccaaag atgagggcgt agcggcgagc catcgcggtt cgctcgtaga agcgcccacg
     7021 gcggcgatca gtccagtaaa ccttgaacgg gcgattgaac aggaaaatga aaaacgcggc
     7081 gatcaccagc gccagccccg cccatgtggg cgcgacgagc attcccagaa caataggctc
     7141 ggaaaccagg ctccaacttc cgtgctcggc cgggagagcg acactcttga acgaaacaga
     7201 tcgggttttt gtcttgtccg cgacggcggt cttgttaaca gaggtagatt gttccatatc
     7261 tcaacgcaat tctacatcga attcagttgg cgtaaatacc gcataacttt tcggcaaacg
     7321 tgctataaag gggcatccat cgccgctcgc ggaagaaccg caactacaat gccccggctc
     7381 tttcaatccg ccggcacagg ccatggccgt ttcgctattc acgaccagct tgccgcggcg
     7441 agaccaatgt tccagcatcc cgtccagcac acgagggtcg aggtttaact gccggcttaa
     7501 ttcagtcaaa gtgacaggcc cttcggccgc ttcaaccgct tgcaacacct cacgcaacat
     7561 cagagacctc caacccaaac gcccaggcgg aagacgaaaa aggccgccag ccaggctatc
     7621 gccaattgtc caacgaagct cagccacatc cagcgagtgc ccaattcgtg gcgcatggcg
     7681 gcgatagtgg ccatgcaggg cgtatagagc agcacgaaga ccatgaaggc gagacccgcc
     7741 gcggcggcat gcccaccgct cgattctgta aagctcgccc tgatggcgtc tgccaggcct
     7801 ggaacggcct catcatcgcc cacggataaa ttaatcccaa tcaatcccgg tatggctttc
     7861 agggtatcga gtatcgctcc caagaacgaa gtgactatct cgcccaggtc ctgcaaaaat
     7921 gtatcgctcc tcgcttcgtc tattgcgccg cttgtgccat acgtctgcgc caacgtgctg
     7981 acaacgactt ctttggcgac caggccactg ataagcgccc caccggactc ccacgtgccg
     8041 aaaccaagcg gctccagcac cgacgccatg gcattggagg cgcgaccaaa aacgctatcg
     8101 gccatcgcta cgttggcgaa cgtcccgtgc gcaccggccg ggatggcagt cagaaaccag
     8161 atggcaatcg tcgtgacaag gatgagcgag gccgcgtgct gcaaaaaggc tcgtgtccta
     8221 atccacatct ggacaccgat gccgcgcgcg gtgggcagtc gatagggtgg caactccatc
     8281 accaggcccg gcgccgcggc tgcgggtagc aatgaatgct gcaatagcaa gccgacgcca
     8341 atggcgatga tgacgccaag cagataaagc cccataatca cgacgctggc gtaacggggg
     8401 aaaaatatag ccgcaaaaag aacatagacc ggcaggcgcg cgccacaact catgaacggc
     8461 accaataaac cggtcatgat gcggtcccgc tcgttggtca gggtgcgcgt ggcataaata
     8521 gcgggcacag aacaaccaaa gccgaccatc aacggcagaa aactgcgccc atggagaccg
     8581 atgcggttca tcaggcgatc catgacgaac gccccccgcg ccatgtagcc ggaatcttca
     8641 agcacagcca gcgcaaagta gagcgccatc agcaccggaa caaagaccag cacaccccca
     8701 actccggcaa tcacgccgtc tatgagcaga gaggctaccc atgtctctcc aaggcctatc
     8761 gcgtcaagaa ccgtcataat ccagcgactg atggggccac tgagaacgcc gtcgatccag
     8821 tcaacccacg gggcagaaag atcggtggtg atcttgaaaa ccagccacat cgccagcata
     8881 aaaatgggga tgcccaaccg gcgatcggtc atgacatgat cgatgcggtc ggagtttgac
     8941 atagtgtggg ctgaaccagc gcgcaccaca cgccccgcca gattatgaat ccaggtatac
     9001 cgccgatcgg ctatgaacag atcgatatct tccccaagcg cggcggccaa ctccgcgcgc
     9061 cgtttgcccg cggccggcag aagctcagag ccaccaggca agcccgaaag acgcgccgcg
     9121 acatcgacat ccccttccag aaggcgcacc gccagccagc gcggcgcgta atattccctg
     9181 atggcgatat agtcgttgat aatcaccagc aggctggcga tggctctttc gatcgcctcg
     9241 ccgtaatcga gggagcaggc ggaagtcggt aaagtcgagg catgggaaac ggccgccaca
     9301 atctcatcga tccctctctc ctggcgagcg atagaaggaa taaccggcac attcagggag
     9361 caactcaatt caggaatgtc gatttgcagc ccttgcttcg cggcgacatc catcatgttc
     9421 agagccacca ccgtgggcag ttccagttca agtaattgca cgatcagata caaattgcgt
     9481 tccagattag tggcgtcagc cacagcgatg atcgcatccg ggcgtgaatc gagcagataa
     9541 tcgcgcgaca caagctcttc catcgtgtag gcgctgagac tatacgtgcc gggcaaatca
     9601 gtaatggcta ttcgacggcc atggcgcgca aattcgccgg tcttctgctc aaccgtcttg
     9661 ccgggccaat tgccgacgtg ctggcgtgcg ccggtcaacg cgttgaagag cgtgctttta
     9721 ccggcattgg gattcccggc caaagcgact gataatacgt tcgattcggt ggagagcagg
     9781 tgaggcgtca ttacggaatg gcgtcttcga aggtttatcc gcaggaaact ttaccggatt
     9841 cccggtcggg caattcgacc cagacgcgcg cagccaggtc ccgccccaac gcgacgcgcg
     9901 agccgcgaac cgacagcaac atcgggccac cggcatcctg aaccactcgg accctcgtgc
     9961 ccggtgtcaa ccctaactca agcaggcgat ggaccacaca atgatcgacc aacagacagt
    10021 gcaggcaagc atcccggccg cgcgggcaat cgacaagcgg tagaaagccg gcggccgtca
    10081 atccagccac acgttcatcc ataacgggta gcggccgcgg cgtggaaacc cggcgcacca
    10141 caaacatgac ggtcgccaga gcgaggatag ccacaacgat ccacagggta acagggccgg
    10201 tcattacagc gcatctccca attgcgtaga tgcccgctcc gagacttcct ggtcatacac
    10261 atcggccaca gccagatgaa ccgtattaag caaatcgttc acccgacgaa gcagccgcat
    10321 gttcgcttcg ggcctgatgt tggcggactc aggcaactgc agggccgtct cgataaggct
    10381 atcccggaaa aagatcgacg cctccagcgt ggcgcgcagg ggcaatccct ggttcctggc
    10441 attttcagca tacagccggc cgattcgccc ggcctcttcg agaatcgaag cgccggcttc
    10501 ctcgctggat tcgctggaaa gatattgcag tgtcaacccc ataagttgct ggcctagttt
    10561 ccggttctga tttcgggctt cttcatcgaa ataggtcagc cagccttcct tccggtcagc
    10621 catcgtctcc tggcgtgtat aagccagcgc cttcgtcgcc cacaagccgg tcaggctgtt
    10681 cacattgcgc agagcgctac gctcgcgcgc gaatcgcgcc agatcggtcg cggcaaaccg
    10741 ccggtggccc ccgggggtca ccagcgtggg gatatcgccg ctgtcagacc atcgtcgcaa
    10801 cgtggtgggg tgaacgttca atagttcggc cgcttcgctg agcgtgagcc aagt
//
LOCUS       DAOMQG010000084        10820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2609_length_10820_cov_11.849048, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000084 DAOMQG010000000
VERSION     DAOMQG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2609_length_10820_cov_11.849048"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..658)
                     /locus_tag="PLC06_06630"
     CDS             complement(89..658)
                     /locus_tag="PLC06_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRO90155.1"
                     /translation="MPRRDFQSPDDLVVWLVASGIDVSEWGNGDSKTTADLWHEFVVG
                     ESSFIDYPPGRLVEVAQLLIRRGDAILIEIEQEFADGRRRVRMRPPSEKLVRGEEPRT
                     AAIRCLKEELGLSSAEVTVGADHEITEESVDSPSYPGLPTHYRFHTFEVATDSLPDGD
                     FYRENIAPGDPVRRHLWGWRAEWAVNSIQ"
     gene            complement(660..1775)
                     /locus_tag="PLC06_06635"
     CDS             complement(660..1775)
                     /locus_tag="PLC06_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRO90156.1"
                     /translation="MQIKSFATELFFNLYEFTTPHLLCASDCETMSVSELLRLAGAKP
                     DELLDLRLGYTESSGHPHLRTAVSKLYERTSADEIVILGAPEEGIYLTMRTLLEPGDH
                     VIVLTPAYDSLLNLAEHISGNVSRWPIRPTSGGWALDLDELERLITNDTRLVVVNFPH
                     NPTGYLPTAAEFNALINIVRRRGAWLLCDEMYRGLERDPAGRPPSAADRYERAIVLSG
                     LSKTYGLPGLRTGWLVVGDPATRAEIINWKHYTTICAAAPSEMLATIALSIHETLAAR
                     NRQIIAANLDTAAAFFDRWPALFDWRPPQAGSVALVGIDVPSATNYCHALARDAGVLL
                     LPGPCLGADDHSVRFGFGRLGFRESLAAYERYLESER"
     gene            1928..2887
                     /locus_tag="PLC06_06640"
     CDS             1928..2887
                     /locus_tag="PLC06_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90157.1"
                     /translation="MTRKVIFVGGTSYSGSTFFHLTLANDPKGFAVGEVKHLFRPTKE
                     RHARPTWACGCGDPECTLWNRVRKNGEAHLYESIFEIHPEVEFIVDASKNVVWIDDQI
                     DYLSRQGIEVQSVVIWKTLMEFAHSLKKRNRLGAKDGAELANWPRYHRNFYSFVEDFR
                     AVKYADYARDQAAVLQAACAYFDIPYFEGKERYWEKVHHSLGGNLSSRIHLYSKESDR
                     FQDVQQRAKGHRNDHVADDSNYRQVYYERPDEATLETHIARLRRDNPYLDSVEEMLAA
                     HDVAGTTLPSGEWPELKLGMVDMRMKQLRQFTRTRIGRLRFAQ"
     gene            2902..3840
                     /locus_tag="PLC06_06645"
     CDS             2902..3840
                     /locus_tag="PLC06_06645"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRO90158.1"
                     /translation="MSSTSLTHRNGREEPQLAGNIMRLPNFIIGGVIKGGTTSLNYYL
                     KQHPDVFMSRFKEPRYFAYEPDNPDHVEGRGLRFPIRTLTEYAALFEEATTERAIGES
                     SPHYLRSPQAPGLIKETIPDVRLIFSLRDPVKRAYSSYWHKVRLGIEDRPVEDALMEQ
                     DQAVQHGLYSQSLEKWNALFDPAQIKIILFEDLIRDTLGVFADLCRFLEVDDTFVPDL
                     VVRNKGGAMKNQRLGRVYEQIKTHPLRQAIDPFVPAKLRQMMINTRNNNLEEPPPMPP
                     LIAQRLYDFYREDVEKLESMINRNLSIWKNQAAPGR"
     gene            complement(3891..5879)
                     /locus_tag="PLC06_06650"
     CDS             complement(3891..5879)
                     /locus_tag="PLC06_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRO90159.1"
                     /translation="MATYYVDGNSGNDSNSGSSDSPWKRLEKALSKVQPGDEVRVRTA
                     TYNEELALTVRNTTWKADTGHKPVLDGRYHEGLFRPDRTLPTPVPGSGYLPDGLGNMV
                     TLREEGIVVDGFTIQNCSGTGIGITSSNTIIRNCRVDFTYDSSIKVNPSTSYIDNVII
                     ENNVCTRSSMRYFDPGRNPTGGPQSVNGVMKIGRTRDGIIRNNIFAYGHGEGINVDKG
                     SYRILVEGNVVHTCNHVHIYINRSIDPIIRNNLVYHLHTADYLGPNKRPPAGIIYGDE
                     YAQGRPWEHSSGGQIYNNIVVGLGRLFMIRNNENNYDTQLKDAYIGFNTFIGGPLTEV
                     GIQIAGNLKGRPHNNSIFENNIITNVSVISQVSGDLKGILFRNNLWDEQPHAALRGPG
                     DRIGDPNLVNPNAKIKDLFPDPGNNIDPRNYQLTSRSSLAIGMASDGSQANSIKPPAI
                     TKDFFGANRDSKPDIGAHEYAGVVTALTANFSIGPGQDAGSIPHTVDFIDKSISEGVI
                     VSRLWDFGDSGTSSETNPSHTYATAGTYDITLTVTDDKGNTDTMVQSGLITALSEPDT
                     PVPDSFRRFILIQKDNQQVMAYGTQYPDLRCVVIWKIDPFHLMNFNDIGDVVRNVHVD
                     DTNEILWIDPGDLDEPVLAYDDSEMFGKSEPISTLFRS"
     gene            complement(5946..8489)
                     /locus_tag="PLC06_06655"
     CDS             complement(5946..8489)
                     /locus_tag="PLC06_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO90160.1"
                     /translation="MGTTYFVSAADGNNNNPGNQQDKPLRTIQAAINKAQPGDTISVR
                     TGTYAERLHIQKPGTVDSPILITAFQNEKPVIDGIGLNLANDSALVIIQQSQDVQLAG
                     FTIRNSGGRGILISKSSRIIVRNVTIESCYAGGLHARQVDKLLVEGSHIHNCARRFLA
                     YGPEQLNVALLVRGCQDVVIQNNQVYENSDEGIAIGLGCRQVIVRKNTCYDNRNGQIS
                     VTSAVEVGVDSNLCYHTGRADFLTLNGERGPGIVKDDLWRYQTTGKWHTRDVNFINNI
                     IVGCGVGFSAVRRGGKLSKIQVAHNTILNSSKHAIYLKARARSTMSFIENNLFASNSG
                     GEMAYTLGGEGIVWRHNLWSSFPGENIYNPSNDVVEVETGLANPAAPVEPGAVTADSY
                     KLVSTSRAINNGIRNKDETSNDFWGSPRDAKPDIGANEYPAGSADPPTGDDPALPPVG
                     TRVTKGLASLYEFKEKQGREVHDTSGTSDALTLYIKDESRVTWTEDGLLIKEAVLISS
                     ERPATKIIKACRQSNEITLEAWIRPTNTSQDGPARVISISNSKTQRNATLGQGLHGNR
                     PADLFMARLRTTRTSTNGLPAVVSPPGTASSDLTHIIYTRHIDGRSVLYINGQERGIL
                     DVDGDFSNWDETMPLLLGDEVSEDRPWLGLLHLCAIYSRALAPTEVLHNYEANYQGDV
                     NLSADFSILPEDEYGIIPHVVEFDSGDSIATAGITGYFWEFGDGQTSNRANPIVTYTA
                     PGVYSVSLTITDANGQTDKLTKEGLITVVEKPVAPLPAEYARFILVNITDSKVMAFGI
                     QYPDLRCSLLWNDEPYHMIVYADVDDVKRVYTAGNMVELIWVDRLEEEY"
     gene            9177..9968
                     /locus_tag="PLC06_06660"
     CDS             9177..9968
                     /locus_tag="PLC06_06660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRO90161.1"
                     /translation="MNNLQDEDGTLKEMEEQKVLQWTMGPAISSEREGADSFYFVAKR
                     IMDLAIVVLSMVFLLPLMAFIAILIKWDSPGPAIFRQERVTARRRVRNGQVYWEEAPF
                     TIFKFRTMRTDAKSTIHRQFIEAYIAGDDKRMADLQSAQQAEDAKYKLVEDPRVTRVG
                     SFLRKTSLDELPQFLNVLLGNMSLVGPRPPIPYEVELYLYHHHDRLRTVPGITGWWQV
                     KGRSATSFEEMVRMDVDYIRRQSLWLDIKIIIMTVTAVVNGKGAR"
     gene            9970..>10820
                     /locus_tag="PLC06_06665"
     CDS             9970..>10820
                     /locus_tag="PLC06_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRO90162.1"
                     /translation="MTDLRIGAIGCGYWGPNLIRNFIEIPGAQVIAISDLQQEPLNRM
                     MQRFPQIQTSTRNYRDLFEMNLDAVVIATPPATHYEIARDCMEHGLHVLVEKPITLDS
                     EDAAKLIRVAEKNNVVLMVGHTFEYNSAVRAVKEMILSGELGDIYYIDAIRASLGLFQ
                     TKANVVWDLAPHDISILRYLLDADPISVSTHGSSCVQEGIEDVAYTTLMFPNNIIAHI
                     RSSWLDPSKQRRITVVGSKKMVIYDDVEPLEKIKIYDKGVKAIRHTDTFGEFSFAYHY
                     GDVVIPY"
BASE COUNT         2364 a         2853 c         3051 g         2552 t
ORIGIN      
        1 ctactgatcc actgacctac tgatccactg acctactgat ccactgacct actgatccac
       61 tgacctactg atccactgac ctactgacct actgaatact gttcactgcc cactccgccc
      121 gccagcccca tagatggcga cgaacaggat ctccgggggc gatgttctcg cggtagaaat
      181 ccccgtccgg cagactgtcg gtggctactt cgaaggtgtg gaatcggtag tgcgtgggca
      241 ggccggggta tgagggggaa tcaacgctct cttctgtaat ctcgtgatcg gccccaaccg
      301 tcacttcggc cgacgataac cccaactcct ctttcaggca gcggatggcg gccgtgcgcg
      361 gctcctcgcc gcgcacaagt ttctccgaag gcggccgcat gcgaactcgt cggcggccgt
      421 cggcaaattc ctgctcgatc tcgatcagga tagcatcccc ccgtcgaatc aagagctggg
      481 ctacttcaac cagccggccg ggagggtagt cgatgaagga ggattcgccg acgacaaact
      541 catgccagag atcggccgtg gtcttgctgt cgccattccc ccactcggag acgtcgattc
      601 ccgacgcgac caaccagacg accagatcat cggggctttg aaaatcacgt cggggcattc
      661 tatctctccg attcaaggta ccgctcgtag gcggccagcg attcccggaa cccaagccgg
      721 ccgaagccga agcgcacgct gtggtcgtcg gctcccaggc atgggccggg cagcagcaac
      781 acgcccgcgt cgcgggccag cgcgtggcag tagtttgtgg ccgagggcac gtcgatcccg
      841 acaagggcga cggacccggc ctgcggtggc cgccaatcga acagggccgg ccaacggtcg
      901 aagaatgccg cggccgtatc cagattggcg gcgatgatct gccggttgcg tgccgcaagg
      961 gtctcatgga tgctcagggc gattgtcgcc agcatctcgc tgggcgcggc ggcgcagatg
     1021 gtggtgtaat gcttccagtt gatgatctcc gctcgcgttg ccggatcgcc cacgaccagc
     1081 cagccggtgc gcaggcccgg cagtccgtag gtctttgaca gccccgacag gacaatcgcc
     1141 cgctcatatc gatcagcggc cgaaggcggc cggccggccg gatcacgctc cagcccgcgg
     1201 tacatctcgt cgcacaggag ccacgccccg cgtcggcgca cgatgttgat gagcgcgttg
     1261 aactcggccg cggtgggtag atagccggtg gggttgtggg ggaaattgac gacaacgaga
     1321 cgggtatcat tcgtgatcag ccgctccaac tcatccagat cgagcgccca gccacctgaa
     1381 gttgggcgga tcggccagcg gctaacgttg ccactgatat gttcggccag gttgagcagc
     1441 gaatcatagg ccggggtcag gacgataacg tggtcgcccg gctccagaag tgtgcgcatg
     1501 gttagataga tgccctcttc cggtgcaccc agtatcacga tctcgtccgc gcttgttcgt
     1561 tcatagagtt tggagacggc cgtccgtaaa tgggggtgtc ccgacgactc cgtgtaaccc
     1621 agccgcaggt ccagcaactc atccggcttc gctccagcca ggcgcagcaa ttcgctcaca
     1681 ctcatcgtct cgcaatcgga agcgcagagc agatgagggg tggtgaattc gtacaggtta
     1741 aaaaagagtt cggtggcaaa tgatttgatt tgcatggggt tccttataat aagggcagta
     1801 atgatacgag ataattgtaa tccggttaga ccatgatggg cgaacgaccg cgttgggctt
     1861 aatatgcgct gtaagtcgta tcgggttaga ttatcatggt gcggccgcgg gtcgccggag
     1921 agattttatg acaagaaagg taatatttgt ggggggcact tcctatagtg gttccacctt
     1981 ctttcatctg actcttgcca acgaccccaa gggttttgcg gttggcgagg tcaaacatct
     2041 ttttcgcccc accaaggagc gtcatgcgcg tccgacgtgg gcttgcggct gcggtgatcc
     2101 cgaatgcacg ctctggaatc gggtcaggaa gaacggcgag gcacacctct atgaatctat
     2161 ttttgagatt catcctgagg tggaatttat tgttgatgcc agcaagaatg tggtctggat
     2221 cgatgatcag atcgattatc tgtcgcggca gggaattgaa gttcaaagcg ttgtgatttg
     2281 gaagaccttg atggaattcg cccactcgct taagaagcgt aatcgactgg gggccaagga
     2341 cggtgccgag ctggcaaatt ggccccggta tcatcgcaat ttttattcct ttgtggaaga
     2401 tttccgcgcg gtgaaatatg ccgactacgc gcgcgatcag gcggccgtct tgcaggcagc
     2461 ctgtgcctac tttgacatac cctactttga aggcaaggag cgatattggg agaaggttca
     2521 ccattcgctg ggcggtaacc tgtcgtctcg tatccatctc tattcaaagg agagcgatcg
     2581 ctttcaggac gtgcaacaac gcgccaaagg ccatcgaaat gatcatgtcg ctgatgatag
     2641 taattatcga caggtgtatt atgaaaggcc tgatgaggcg acattggaaa ctcatattgc
     2701 caggctgcgc cgggataatc cctatctgga ttcggtcgag gagatgttgg cggcccatga
     2761 cgtggccggc actacgctcc cgagcggtga atggccggag ttgaagctgg gcatggttga
     2821 tatgcgcatg aagcaactgc gccagtttac gcgaacgcgg attggcaggt tgcgatttgc
     2881 tcaataagga ttaatcataa aatgagttcc acatctttga cccatcgaaa cggccgcgag
     2941 gaaccccaat tggcaggaaa tatcatgcga ttgccgaact ttattatcgg cggcgtcatt
     3001 aaggggggca ctacttccct aaactactat ctgaagcaac accccgacgt gttcatgagt
     3061 cgtttcaaag agccgcgcta ttttgcctat gagcctgaca accccgatca tgttgaagga
     3121 cgcgggctgc gctttcccat tcgtacactg acggaatatg ccgcattatt cgaggaagcg
     3181 acgaccgagc gagcgatcgg cgagtcctcc ccccattatc tgcgcagccc tcaggctccg
     3241 ggacttatca aagaaactat ccctgatgtg cgactaatct tcagcctgcg cgacccagtt
     3301 aagcgagcct attccagtta ttggcacaaa gtgcggctgg ggatcgagga tcggcccgta
     3361 gaggatgcgc tcatggaaca ggatcaggca gtgcaacatg gcctctatag tcaatctctc
     3421 gaaaaatgga atgccctgtt tgatccggca cagatcaaga ttatcctctt cgaagattta
     3481 atacgcgata cattgggggt gtttgccgat ctatgccggt ttctggaagt tgatgacaca
     3541 tttgtgcccg acctggtggt tcgcaacaag gggggggcta tgaagaacca gcgactcggt
     3601 cgcgtttatg agcaaattaa gacccacccg cttcggcaag ccatcgatcc ctttgtgccg
     3661 gcgaagctgc gccaaatgat gatcaacacg cgcaataaca atttggaaga acccccgcca
     3721 atgccgcccc tcattgctca aaggctgtat gatttctatc gtgaggacgt cgaaaagctg
     3781 gagagtatga tcaatcgcaa tctgtcgatt tggaagaacc aggccgcgcc tggcagataa
     3841 aaaaagaggc gttgggacgc ctctttttat ttgccgaata aggttatctg tcagctacga
     3901 aacaacgttg agattggttc cgactttccg aacatctctg aatcgtcata ggccaatacc
     3961 ggctcgtcta aatcaccggg atcgatccac aggatttcat ttgtatcgtc tacgtgaacg
     4021 ttgcgcacga catcccctat gtcgttgaag ttcattagat ggaatggatc aatcttccag
     4081 atgaccacac aacgaaggtc cgggtattgg gtcccatagg ccatgacttg ctgattgtcc
     4141 ttctggatga ggatgaaacg acggaaagag tcagggacgg gagtatccgg ttctgatagc
     4201 gcggtgatta gcccgctctg gaccatcgtg tcggtgttgc ccttatcgtc ggtgaccgtc
     4261 aaggttatgt cgtaggtgcc cgctgtagcg taggtatggg atgggttcgt ctcggatgag
     4321 gtgccgctat cgccgaaatc ccatagccgc gacacgatga caccttccga aatgcttttg
     4381 tcgatgaagt cgaccgtgtg ggggatagaa ccggcgtcct ggcctgggcc gatgctgaag
     4441 ttggcggtca gcgctgtgac gactccagca tattcgtggg caccaatatc gggtttgctg
     4501 tccctgtttg ccccaaagaa gtctttggtg atggccggcg gtttaatact gttggcttgt
     4561 gagccgtcgc tggccatacc gatagccaat gaactgcgcg atgtgagttg gtagttgcga
     4621 ggatcgatgt tgttgccggg atcgggaaac aggtctttga tcttggcatt cgggttaacg
     4681 agattgggat cgcctatccg atctcctggc ccgcgcagag ctgcgtgagg ttgctcatcc
     4741 cacaagttat ttcgaaagag gatacctttc aaatcgccac tgacctggct gatcaccgac
     4801 acattggtga tgatgttatt ttcaaagatg ctgttattgt gtgggcgacc cttaagattg
     4861 ccggcgatct gaatacccac ctcggtcagc gggccaccga tgaatgtgtt gaagccgata
     4921 taggcgtcct tgagttgggt gtcgtagttg ttttcgttat tgcggatcat gaacagtcga
     4981 cccaggccaa ctacgatgtt gttatagatc tgaccgcctg agctatgttc ccatggtcga
     5041 ccctgagcat attcgtctcc gtaaatgata cccgccggcg gacgcttgtt tgggcccagg
     5101 taatcggcgg tatgcaaatg atagacgaga ttgttgcgga tgatcggatc gattgaacga
     5161 ttgatataaa tatgaacgtg gttgcacgta tgaaccacgt tgccttcgac caggatacga
     5221 taactgcctt tgtcgacgtt gatcccttcg ccgtgaccat aagcaaagat attgttgcga
     5281 atgatgccgt cgcgcgtgcg gccgatcttc atcacgccgt tcacactctg agggccgccg
     5341 gtcgggttgc gacccggatc gaaatagcgc atgctggagc gggtacagac gttattctcg
     5401 ataataacgt tgtcgatata ggaggtagag gggttgacct tgatcgacga atcgtaggtg
     5461 aaatcgacgc ggcaattgcg aatgatggta ttgctgctgg tgatcccgat ccctgtgccg
     5521 gagcagtttt gaattgtgaa gccatcgacg acgatccctt cctcacgaag ggtgaccatg
     5581 tttcccagcc cgtcgggtaa ataaccggag ccgggcacgg gggttggcaa cgtcctgtcg
     5641 ggacgaaaga gaccttcgtg atagcggccg tcgagcacag gtttatggcc cgtatccgct
     5701 ttccaggtgg tgttgcgaac cgtgagcgct aattcttcgt tgtaggtggc tgtccgaact
     5761 cgcacctcat cgcccggctg gactttcgaa agcgccttct ccagccgctt ccatgggctg
     5821 tcgctgctgc cgctgttcga atcgttgccc gaatttccgt ctacgtaata agttgccatt
     5881 gaatcctacc ttatcaagct atccttaccg gccgggtttc gatccggccg actctgttct
     5941 cggatttaat actcctcttc caatcgatcg acccaaatca attccaccat attgccagcc
     6001 gtgtagactc gctttacatc gtcaacgtcg gcgtagacga tcatgtgata tggttcgtca
     6061 ttccacaaaa gggagcagcg caagtcggga tactggatac caaacgccat aactttggaa
     6121 tcggtgatat tgaccaaaat gaaacgcgca tattcggccg gcagtggtgc gaccggtttt
     6181 tcgaccaccg tgatcaagcc ttccttggtg agcttatcag tctggccgtt ggcatcggtg
     6241 atggtgagcg agacggagta gacgcccggc gcggtgtagg tgacgattgg gttggcccgg
     6301 ttggaagtct gtccgtcgcc gaactcccag aaatagccgg tgatgcctgc ggtcgcaatg
     6361 ctgtcgccgg aatcaaattc cacgacatgg gggatgatgc catattcatc ctcaggcagg
     6421 atagagaagt cagccgagag attgacatca ccctgatagt tggcctcata attatgtaat
     6481 acttctgttg gagccagtgc gcgggagtag atggcgcaca agtgcaacag gcccaaccat
     6541 ggcctgtctt cactcacttc gtcgccgagg agcaggggca ttgtctcatc ccagttgcta
     6601 aaatcgccat cgacatccag gatgcctcgc tcctgaccgt tgatatacag gacagatcgc
     6661 ccgtcaatgt gtcgggtata gatgatgtga gtcaagtcgc tcgacgctgt ccctggcggt
     6721 gagacgactg cgggcaggcc attggtcgag gtccgggtgg tgcgaaggcg agccatgaac
     6781 aggtcggccg ggcgattgcc atggagaccc tgacccaggg tggcgtttcg ttgagttttg
     6841 ctattggaga tgctgatgac gcgcgcgggg ccgtcctggg aggtattggt cgggcgtatc
     6901 catgcctcaa gcgtgatctc gtttgactgg cgacaagcct tgatgatttt ggtagccggc
     6961 cgttcggagg agatcaggac cgcttctttg atcaacaacc cgtcctcagt ccaggtaacg
     7021 cgcgattcgt ctttgatata caaggtgagc gcgtcgctag tcccgctggt atcatggacc
     7081 tcgcgacctt gcttctcctt aaattcgtaa agagaagcca gaccttttgt cacccgtgtg
     7141 ccgacgggcg gcaatgccgg gtcgtctccc gtgggcggat cggcgcttcc cgcagggtac
     7201 tcatttgcgc cgatatccgg ctttgcatcc cgtggcgaac cccagaaatc attcgaggtc
     7261 tcgtctttgt tgcggattcc gttgttgatc gcccgcgaag tgctgaccaa tttgtatgaa
     7321 tcggccgtca ctgcgccggg ttcaacgggt gcagctggat tggccaaccc cgtttccact
     7381 tctaccacat catttgaggg gttgtagatg ttctccccag ggaacgacga ccatagattg
     7441 tgtcgccaga cgatcccctc tccaccaagg gtataggcca tttcgccacc tgaatttgag
     7501 gcgaagaggt tgttctcgat gaaagacatt gtgcttcgcg cacgtgcctt tagatagatt
     7561 gcatgtttgc tgctgttcag gatggtgttg tgggcaacct ggatcttgct cagcttgcca
     7621 cctctacgaa ctgcgctgaa gccgactccg cagccgacaa ttatattgtt gatgaagttg
     7681 acatcgcgtg tatgccattt accggtcgtt tgatagcgcc acagatcatc cttgacgata
     7741 cccggaccac gttctccgtt caacgtcaga aaatcggctc gaccggtgtg gtagcagagg
     7801 tttgaatcga cgcccacttc cacggcagaa gtgacgctga tctgtccatt gcgattatca
     7861 tagcaggtgt tcttgcgcac gatcacttgc cggcagccca ggccgatagc gatcccctca
     7921 tcagaattct catagacctg attgttctga attacaacat cctggcagcc gcgtaccaaa
     7981 agagctacgt tcagttgctc ggggccatag gccagaaaac ggcgcgcgca gttgtggatg
     8041 tgcgaccctt ccaccagaag cttgtccact tgcctggcat ggaggccgcc ggcatagcat
     8101 gactcaatcg ttacattgcg aacgatgatc cggctcgact tggagatcaa tatgccgcgg
     8161 ccgccggaat tgcgaatggt gaacccagcc agctgcacat cctggctttg ctggataatc
     8221 accagcgcgg aatcgttggc aaggttgagg ccaatgccgt cgattaccgg cttctcattc
     8281 tggaatgctg taatcaggat tggcgaatcg accgtgcccg gcttctgaat gtggaggcgc
     8341 tcggcgtatg tgcctgtgcg aacggagatg gtgtctccgg gttgcgcttt gttgatcgct
     8401 gcctgtattg tgcgcaacgg cttgtcttgc tggtttccgg ggttgttatt gttcccgtca
     8461 gccgctgaaa cgaagtatgt agtgcccatg gctctgaatc tccctgacta ttcgggtgag
     8521 aatgcataaa acatcctccg cgccatcggt cgaacaggtc actgtcattg tgctcctgtt
     8581 tgccgacggg cgcgtaagcg cgccataacc attacatcgc ttcagaactt caatgccgat
     8641 acggtgaaaa gtaatttggg tgatgccgga aggcgactca gtggagcagg ggatggattg
     8701 aagaggacgt ccgctgtttc ccgaactgcg ggctttcccg catagcggac ccatcccgct
     8761 ggtcacgttg aatgatcact cacggcgggg gcatcatcat aactacttat cgatgaatta
     8821 taagtgccac ccgccggtca tgcaagcaaa tattgtacta atacaatatg ttgtaatgta
     8881 aaaatattga actacggcaa catcatattt cgattcaacg tcatacctca gattggaaat
     8941 tgctgccatt cttaacatgc gtcgtaaggt gtgatagcta ggatgagaat taagtcgatt
     9001 actcttcaat ctatggatag aaccttttgg atagaccgta ccccgcaaaa cttcgagaaa
     9061 cccgctcaat ccagggatgt gaattctatc agttgatagt gctatcgtta gcctttttgt
     9121 aagttttatt gttgagaata ggcccaggct gtggaatcaa ctacaggttt ggtttgatga
     9181 ataacctgca ggatgaagac gggacgctga aagaaatgga agagcaaaaa gtcttgcaat
     9241 ggacaatggg accggcgata agctccgagc gagaaggggc cgattcattc tatttcgtcg
     9301 ccaagcgaat catggatctg gcgattgtcg tgttgtcgat ggttttcctg ctccctctca
     9361 tggcgtttat cgccatcctg attaaatggg attcacccgg gcctgcgatc tttcgccagg
     9421 agcgggtcac ggcccggcgg cgggttcgca acggccaggt gtattgggaa gaagctcctt
     9481 tcaccatttt caagtttcgt accatgcgaa cagatgccaa gtcaaccatc caccgccaat
     9541 tcatcgaagc ctatattgcc ggggatgaca agcgtatggc cgacctgcaa tcggcgcagc
     9601 aggctgagga cgccaagtac aagctggtgg aagaccctcg tgtgacgcga gtcggcagct
     9661 ttctgcgcaa gaccagcctc gacgagttgc cccagttcct gaatgttttg ctgggtaaca
     9721 tgagcctggt tgggccgcgg ccgcccattc cttacgaagt tgaactatac ttataccatc
     9781 atcacgaccg cttgcgcacc gtgcccggta tcaccggttg gtggcaggtg aaaggtcgca
     9841 gcgcgaccag cttcgaagag atggtacgca tggatgtcga ttacatccgc cgccagtcct
     9901 tgtggctgga tatcaagatt atcatcatga cggttacggc cgtcgtcaac ggtaaaggtg
     9961 ctcgctaaca tgaccgacct tagaatcggc gccattggct gcggctattg gggccctaac
    10021 ctcattcgta actttattga aatccctggt gcgcaagtta ttgccatttc cgatctccag
    10081 caggagccgc tcaaccgcat gatgcagcgc ttcccgcaga ttcagacatc gacgcgcaat
    10141 tatcgcgatc tgttcgagat gaacctcgat gccgtcgtga tcgccacgcc gccagccacc
    10201 cattacgaaa tcgctcgcga ctgcatggag catggtcttc atgtcctggt ggagaagcca
    10261 atcaccctcg acagtgagga tgcggccaag ctcatcaggg tggcagagaa gaacaatgtc
    10321 gtcctgatgg tcggccatac cttcgaatat aactcggccg tccgcgccgt taaggagatg
    10381 atcctcagcg gtgagttggg cgacatctat tacatcgatg ctatccgcgc cagcctgggt
    10441 ctgttccaga ccaaggctaa cgtggtttgg gacttggctc cccacgatat ttccatcctg
    10501 cgctatctgc tggatgccga cccaatcagc gtcagcaccc atggctcatc gtgcgtgcag
    10561 gaagggatcg aggacgtggc ctatacgacc ctgatgttcc ccaacaacat catagctcat
    10621 attcgctcca gctggcttga cccgtcgaag cagcgccgca tcaccgttgt cggcagcaag
    10681 aagatggtca tctatgacga tgtggagccg ctggagaaga tcaagatata cgataagggc
    10741 gttaaagcca tccgccatac tgacaccttc ggggagttca gctttgctta tcattatggc
    10801 gatgtggtga tcccctatat
//
LOCUS       DAOMQG010000085        10775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2624_length_10775_cov_10.640112, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000085 DAOMQG010000000
VERSION     DAOMQG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2624_length_10775_cov_10.640112"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLC06_06670"
     CDS             complement(<1..847)
                     /locus_tag="PLC06_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90163.1"
                     /translation="MKHYVVTDWVETAQLWARRHALPIFFIALTIGLTLILSFDLPGS
                     NQVIAIVGQPAQNDIFSPRSMTYTSDLLTNQAREQAGRIVNEVFTPLDLAIGRSQLTL
                     AREVFTFIEAVRADSSATIGRKLEYLTAIEGLTISEAGGQSLLDLSQPDFDEVRANVF
                     AIIEDLMRQEIRPAQISDFQRTARRLVSLELSTAQTSVVAELAPQFIIPTVFPDEEAT
                     ASARSEAAAAVPPVSRTVARDQRIIRAGEIVTDTDYELLGELGLLQQSTNWLHALSMG
                     LISLGI"
     gene            complement(878..1321)
                     /locus_tag="PLC06_06675"
     CDS             complement(878..1321)
                     /locus_tag="PLC06_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRO90164.1"
                     /translation="MDLKEKLRADLATAMREGDSQRRDVLRMVLAAVKQVEIDDRKTL
                     DDDGVQEVLRKQVKLRQESIVDFEKAGRPSEVERERAEIVIIEGYLPQMMSREEIEQI
                     ARGIIDELGVTNAKAMGQVMSRLMPYVKGKADGRVVNEVVRSLLQ"
     gene            complement(1371..2072)
                     /locus_tag="PLC06_06680"
     CDS             complement(1371..2072)
                     /locus_tag="PLC06_06680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90165.1"
                     /translation="MDDLLVRKLVELNTDFYVRFAAPFAASRSAPQPGFFSLLEDLPA
                     RPLSVLDVGCGNGRLGRFLHGAQRLDYYVGVDATQALLDQIVDFDGELYIRDISLPDS
                     LQGLEKFDLVVCLATLQHIPGQENRLRLLREMATHLEPSGLLVLSNWQFLNSFRQRQK
                     IRPWALVDIDPSQLEPNDYLLSWERGGSGVRYVAYLDGKSIEEMAIAADLRIVKQFIR
                     DGREGNLNLYTILAS"
     gene            complement(2151..2606)
                     /locus_tag="PLC06_06685"
     CDS             complement(2151..2606)
                     /locus_tag="PLC06_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90166.1"
                     /translation="MAAPNTVQYSTLQDKVYGDFGLEVDVTQIAGPPESSYGVLFRMT
                     DGQQFYRFDITGNGLYIVERHNGDGTWTRLVQEWTPTSALNQGLNVTNRLRVLASGQS
                     LAFYANDVLLTQVNDSTFTGGMIGLDAGTFGGSNLQVAFDNVMITGSAP"
     gene            complement(2818..3486)
                     /locus_tag="PLC06_06690"
     CDS             complement(2818..3486)
                     /locus_tag="PLC06_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90167.1"
                     /translation="MKRAYTFWWLSLVIVAVLAAGCRSTSAEPDTCDDPPQILFFDDF
                     NGEQDCGWATYNRGGGIAAIENASMQMTVSQPGQIWWTNPTRNFDDVIIRAEARQVSG
                     PNDNAYGLICRYQDPKNFYAFLVSGDGYYVIAKYQSGSENVIYLTENGQFQPSEAIHT
                     GVASNELLASCIGNQLSLEVNGTPLITVTDPTFVTGDIGLAASTLQAETGVIEFDNVQ
                     VSAP"
     gene            complement(3494..3640)
                     /locus_tag="PLC06_06695"
     CDS             complement(3494..3640)
                     /locus_tag="PLC06_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90168.1"
                     /translation="MIAALIVIVLWTLIIGIFLTVSRRQPDVQTQIKAVEERLDSIEK
                     KSRS"
     gene            complement(3753..4316)
                     /locus_tag="PLC06_06700"
     CDS             complement(3753..4316)
                     /locus_tag="PLC06_06700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRO90169.1"
                     /translation="MPRLTKIYTRRGDKGLTSLSGGQRVPKNSLRVSAYGTVDELNSA
                     IGVALSFGLCERLASVLPTIQNELFHLGSDLSFLEEDKVKYPVPQVKAEHVTQLEKLI
                     DEMNAVVGPLQNFILPGGSPGSSLLHVARTVCRRAERDVVTLAEEEAIGPYVIQYLNR
                     LSDLLFVMARYENHSRGVAEPLWNSMA"
     gene            complement(4333..5940)
                     /locus_tag="PLC06_06705"
     CDS             complement(4333..5940)
                     /locus_tag="PLC06_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO90170.1"
                     /translation="MIKINRLTYTYPQGVKPALCELDWNVTDGEFVLVAGPSSSGKST
                     LLRCLNGLVPHFSGGAIKGEVEVYGLNAISAGPHVLSRTVGFVAQDPESQTVLDRVES
                     EIAFSLENAAVPREEMRVRVEEVLDLLELTPLRGRAVQTLSGGERQRLAIAAALVQRP
                     KVLVLDEPTSQLDPQSAEDVLRTLIRLNEDLGLTIVLAEHRLERIARYADRLTWIEEG
                     RIRFDGPVREGLERVGTGFPIVQLARTMGWRPLPLTVKEAKQFLPALLVDQSNMTQSG
                     STGQTGDIVLEVKNVDFAYNGRSTLKNIALTVRAGETVALIGRNGAGKSTLLKCIVGL
                     VHTKSGNIRVSGRSIDGRSVADICREVAYLPQNPDDLLFAESVTDELKVTLINHGLTQ
                     ASGDIHEFLRVLGLAEAAEAYPRDLSVGQRQRVAMGAVTVTRPRLMLLDEPTRGLDAS
                     LKAELIALWRKWLADGVAILLVTHDVELAAQIAGRTVMMSQGEVIASGPTREVLGSTP
                     NFAPQISRLLPGRGLLTAADALAYLMN"
     gene            complement(5937..6959)
                     /locus_tag="PLC06_06710"
     CDS             complement(5937..6959)
                     /locus_tag="PLC06_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRO90171.1"
                     /translation="MTLDVRAWVVWLFAGGLLAILTSNPFYLILLLIISRLVEYACAS
                     HIPQQWRLPFWRIAIVILFFSTLFNALTAHFGQTSLFALPAQWPLIGGDITLEAAVFG
                     FLNGLRLITLLSFFLAFNSIVPASQLAGLTPRALHEIGLVLLIAITYIPETIGQYRRI
                     RDAQAIRGYRMRGWRIWHPILIPLLISGLERALNLSETMVARGYGSTTNVSMPARARA
                     AMLAGLLLTLAGALRLVWGGLDGWALIAIGGLAIAGAYLDLSRQVSRTQYYDRRWSLT
                     ATWVVNGALIALGILLVSRRSLNYSPYPDLTAPPFDTWIGAAILGLAVPAIIILFRKP
                     AIEVPA"
     gene            complement(6956..7753)
                     /locus_tag="PLC06_06715"
     CDS             complement(6956..7753)
                     /locus_tag="PLC06_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRO90172.1"
                     /translation="MRSKALSFALYVVAAMIGILALAYPFLIVAFAPLGIGDGSASTT
                     ATPLLTTLLVSLCLAVLLVELQGQAAGAKTVAALGIMVAATSTLRFIEVGIPGPGGFS
                     PIFAPIILGGYVFGARFGFLLGAMTMLVSAVITAGVGPWLPYQMFTAGWIGLTAGLLP
                     HPSHGRAEIFLLIAFAFVWGFFYGAIMNLYTWPYFSGDATTTWAAGIGVGDSIRRYAA
                     FYLTTSLVWDSAAALGNVILMAAFGLPTVRALTRFHGRMQYQAWQAS"
     gene            complement(7753..8922)
                     /locus_tag="PLC06_06720"
     CDS             complement(7753..8922)
                     /locus_tag="PLC06_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90173.1"
                     /translation="MKSPWFLLLLCLTGLIVILMAVDPSWAGNENQVALVVDFGNGQI
                     AARCVSFQEDSITGYEALQRSGLPVETDFQTGGAAICRIDGQGCPSDDCFCACRGGGE
                     CKYWSYWHFVNGTWSYSVAGSGIYKLQDGAIDGWVWGLGSVDQAKPPPAIPFNEICID
                     ENAASPTATSTPRPTSTPMVLPTRAPTENAPNPTATTSIAMATVSVAASPTMPMATTA
                     NPTSGPIAIGNQTETPIPEAPFDAGETPAPVAPPSLGGGESYPGQPGGGLPEAASPEP
                     DAPIITAPQPVGGEMVVVSTPEAIAETGIGGGLIDSNQSTSLATPTSTPISVSAVIGM
                     STTIDNPVIAAATSQVDESSPGISYIGFFGLLLLLAALALLIYRRREMTHPGGNG"
     gene            complement(8968..>10775)
                     /locus_tag="PLC06_06725"
     CDS             complement(8968..>10775)
                     /locus_tag="PLC06_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90174.1"
                     /translation="AASAPAFPDQRSSVQAAVFWMVNEFQNDDGGYASLSTGANQAPS
                     TIPGTLDAILAITAAGHNPAARYPGQEATPLDYLEDNDAALQTFASANGGQAGKVVLA
                     LTASAVDPRDFEDRNFVEILTNSLEPSGAYGVGDPFKQAMAMLGVASVNQTVPESAIV
                     WLESKQGASGAWDDGFGTTNNPDATAMAIMALIAAGREPGTSAIQSAVQFLADAQQED
                     GWGYSAEFTPNANSTAFVIQALSALDEPWYDDGGEWAMNGRSPLQVLLAFQSWSGAFQ
                     SDFGQGPFDDFFATVQAIPAASGRAFPLPARLEAALTALTCLDGMQDTTSGGWPSFAG
                     GSTDASGTSRAIQAIAAAGDDPQSVRWTTSGDTNAVEALEALTPDYLSSGAGGRVGIV
                     MQGVVAAGSPYDVANFAGMNLPEVMAGHLQPSGEYDSTAFGIFAHAEAMLGLLRADEE
                     VAPTAIELLLSSQTNGDWGDSDANGIALQVLGELDSRAPISTLSVLHSEQLTDGSWGF
                     GDVSNPSATSEVVQGLAGIGQNPYGPQWSLVIDGRLANAADVVMGQQGQNGCWPNAYG
                     PGDDPYSTTDAILMLATQPEWGFSLVHLPAIMTQN"
BASE COUNT         2599 a         3167 c         2873 g         2136 t
ORIGIN      
        1 ttatccccag cgagatcaac cccatgctta aggcatggag ccagttcgtg gactgctgca
       61 ggagccccag ctcaccaaga agttcataat ctgtatctgt gacgatctcg ccggcccgaa
      121 tgatgcgttg atcgcgggca acggtacgag atacgggtgg tacggctgcg gcggcttcgc
      181 tgcgggctga ggccgtggcc tcttcatcag gaaacaccgt tggaataata aattgagggg
      241 ccaactccgc gacaacagat gtttgggctg ttgagagttc gagactgacg aggcggcgag
      301 cagtacgctg gaagtcgctg atttgagccg ggcgtatctc ctggcgcatg agatcttcaa
      361 tgatagcaaa aacattcgcc cgaacttcat caaaatcggg ctggctcaga tcaaggagtg
      421 actgaccacc tgcttcactg atggttaacc cctcaatagc cgtcaaatat tcgagctttc
      481 gtccgattgt cgcggatgag tcggcacgaa ccgcctcaat gaaggtgaat acttctctgg
      541 cgagcgtgag ttgcgaacgg ccgatcgcca aatcgagcgg cgtaaagacc tcgttcacga
      601 ttcggccggc ctgttcgcgc gcttgattgg ttaataaatc gctcgtgtaa gtcatcgagc
      661 ggggggaaaa aatatcgttc tgcgcgggtt ggccgacgat ggcaatgacc tgattcgagc
      721 cgggcagatc gaaggacaga atcaatgtca agccgatggt cagcgcaatg aaaaatatgg
      781 ggagcgcatg gcgacgggcc caaagctggg ctgtttcgac ccaatcggtt acgacataat
      841 gcttcatttt aacagcacag agcgtgcagg aagattctca ctgcaacagg ctacggacga
      901 cttcattcac aacgcggccg tcagctttcc ccttaacgta gggcatcagg cggctcatca
      961 cctgccccat ggctttagca ttggtgacgc caagctcatc aataattccc ctggcaatct
     1021 gctcaatttc ctcacgagac atcatctggg gcaggtaacc ctcaataata acgatctcgg
     1081 ccctttcccg ctcaacttca gatggacgcc cggccttctc gaaatcgaca atgctctcct
     1141 ggcgcagttt cacctgcttg cgcaaaactt cctgaacgcc atcgtcatcc agggtcttgc
     1201 ggtcatcaat ttcaacctgc ttgaccgcgg ccaacaccat gcgcaaaaca tcgcgacgct
     1261 gtgaatcacc ttcgcgcatc gcagttgcca aatcagcccg gagtttctct ttcagatcca
     1321 tagctcaagt aatgattact taaaagattg tatcattgcc caatcgggtg tcaactggcc
     1381 agaattgtat agagattgag attcccctcg cgcccgtcac gaataaactg cttcactatg
     1441 cgcaggtcag cggcaatagc catttcttcg atcgattttc catccagata agccacataa
     1501 cgaacgccgc tcccgcctct ctcccatgac aacaggtagt cattcggctc aagttgcgac
     1561 ggatcgatat cgaccaatgc ccagggacga attttttgcc gttgccggaa gctattcagg
     1621 aattgccagt tgctgagcac gagtaatccc gacggctcca aatgggtagc catttcacgc
     1681 agtagtcgta gtcggttctc ttgtccggga atgtgctgaa gggttgccag gcagacgacc
     1741 agatcgaact tttccaatcc ttgcaagctg tcaggaaggc taatatctct aatgtacagc
     1801 tccccatcga agtcgacgat ctgatccaaa agtgcttggg ttgcatccac ccctacataa
     1861 tagtcaaggc gctgggcgcc atgaagaaat cggccaaggc ggccattacc acagccgaca
     1921 tccagcacag aaagaggccg ggccggtaaa tcttcaagaa gggaaaagaa tccgggttga
     1981 ggggctgagc gactcgccgc aaatggagcg gcaaatcgaa cataaaagtc tgtgtttagt
     2041 tcgacaagtt tgcggactaa caaatcgtcc atgccggcgc gacttccagt aattcagcat
     2101 cggctcacgt caaaaccggc agggagagcc gtggccggtg tatccaggtt ctatggcgca
     2161 ctgccggtaa tcatcacgtt atcgaacgcc acctgcaggt tgctgccgcc aaaggttccg
     2221 gcatccaatc cgatcatgcc accagtgaag gtgctatcat tcacctgagt gagcaacacg
     2281 tcattggcat aaaaagcgag tgattgtccg gaagccaaca cccgcagtcg attggtgacg
     2341 ttgaggcctt gattcagcgc cgacgtgggc gtccattcct gaacgagccg tgtccaggta
     2401 ccatccccat tatggcgctc aacaatgtac agcccattgc cggtgatgtc gaaccgataa
     2461 aattgctgcc catccgtcat ccggaacaac accccataac tactttcagg cgggccggcg
     2521 atttgggtta catcaacctc aagtccaaaa tcgccataca ccttatcctg gagcgtcgag
     2581 tattgaacgg tgttgggggc agccactgtg atgagcagct gttcattgac aatggacgta
     2641 gagccaatct cgtcctgctc aatgagccat ttcccggttt ggcccgcgac aaaggcatcg
     2701 gcaaaactcg cgccggcgat ctgttcgcca ggtaccccac cactctgagg cagaagcgat
     2761 ctgcctccac aacccatcaa gagccatatc gtcgtcagga gtaagaggag tattactcta
     2821 tggagcggaa acctgaacat tgtcaaattc gatgaccccc gtttccgctt gcagggtgct
     2881 ggcagccaga ccaatgtcgc cggttacaaa agtcggatcg gttacagtga tcaatggcgt
     2941 accattaact tccaggctca actgatttcc gatacaagat gcaagcaact catttgaggc
     3001 aacacctgta tgaatcgctt cggagggctg gaactgccca ttttcggtca gataaatgac
     3061 gttctcgctg ccactctggt acttggcaat tacgtaatac ccatcgccac tgacgaggaa
     3121 agcgtagaag ttcttggggt cttgatatcg acaaatcaga ccataggcgt tgtcattggg
     3181 accggacacc tgtcgagcct cggcacgaat aatcacatcg tcaaaatttc gcgtcgggtt
     3241 cgtccaccag atttgccccg gctggctaac agtcatctgc atcgaggcat tctcgatggc
     3301 ggcaattccc ccgccccgat tataggttgc ccagccgcaa tcctgttcgc cgttgaaatc
     3361 gtcgaaaaag aggatctgag gtggatcgtc gcaagtatcc ggctcggcac tggtcgacct
     3421 gcaacccgcc gccagaacag caacaatgac cagactcaac caccaaaaag tgtaagctct
     3481 cttcatggct gagtcacgac cgcgatttct tttcgatgct gtcgagccgc tcttcaactg
     3541 ccttgatttg cgtctgaaca tcaggctgcc gccgggaaac tgtcagaaat atcccaatga
     3601 taagagtcca caggacgatg acaatcaggg ccgcgatcat caaatttcca aacatcaaat
     3661 cacctctaca gaccctgatc ggataggcag attccagagt ctgactttga taaatgataa
     3721 caagtcagtg aattgtaatc ggttcgaccc attcaggcca tgctattcca cagaggctca
     3781 gccacaccac gggaatggtt ctcatacctg gccatgacga ataacaagtc cgacaatcgg
     3841 ttgaggtact gaatgacata gggcccaata gcctcttcct cagccagggt aacgacatct
     3901 cgctcggcac gccggcaaac cgtcctggcg acatggagca aagatgaacc cggagaacct
     3961 cccggaagga taaaattctg caacggtccg acgaccgcat tcatctcatc gatcaacttc
     4021 tccagttgag tgacatgctc ggctttaact tgagggacag gatacttgac cttgtcttcc
     4081 tccaggaaac tgaggtctga acccaaatgg aacagctcat tctgaatggt cggcagaacg
     4141 gaagccagcc gctcgcacaa gccgaaggac agggccacgc caatggcgga gttcaattca
     4201 tccaccgtcc cataggcaga gacacgaagg gagtttttag gcacacgctg tcccccggag
     4261 aggctggtta atcccttgtc tccgcgtcgg gtatagattt tcgtcaatcg aggcatgata
     4321 tttctccaga cattagttca tcaaatacgc cagggcatcc gcggccgtca gcaaccccct
     4381 ccccggcaag agccttgaaa tttgcggcgc gaagttcggc gtgctaccca gaacttcacg
     4441 tgtcgggccg gaagctatca cctcgccctg gctcatcatc accgtccgac cggcgatctg
     4501 tgctgccagc tccacatcat gggtcactag caagatcgcc acgccatcag ccagccattt
     4561 ccgccataaa gcgatcagtt cggccttaag cgaagcgtcg aggccacgcg tcggctcatc
     4621 cagcagcata agccgcggcc gagtcacagt cacggctccc atcgccacac gctgacgttg
     4681 cccgacagac aaatcgcgcg gatacgcctc agcagcctcg gccagaccca gcacgcgcaa
     4741 gaattcatga atatcgcccg atgcctgagt aaggccatga ttgatcaggg tcaccttgag
     4801 ttcatcagtc acggattcgg caaataaaag gtcatcggga ttctgcggca gataggctac
     4861 ttcccggcaa atatcggcga ccgaacggcc gtcgatcgat cttcccgaaa cgcggatatt
     4921 cccgctcttc gtatggacca gcccaacgat gcatttgagc agagtcgatt tccccgcgcc
     4981 gttgcgccca ataagggcaa cggtttctcc ggccctgaca gtcagcgcta tattttttaa
     5041 cgtcgatcgt ccattatagg cgaagtcaac gttttttacc tcaagcacga tatcgccggt
     5101 ctgccctgtg gagccggatt gcgtcatatt ggattgatcg acaagcaaag caggcaggaa
     5161 ttgtttggct tccttgaccg tgagcggaag cggccgccat cccatggtgc gcgccaactg
     5221 gacaatcgga aatcctgtac cgacccgttc cagtccttca cggacggggc catcgaaccg
     5281 gatacgccct tcctcaatcc aggtcagacg gtcggcataa cgggcaattc gctccagacg
     5341 atgctcggcc aggacgatcg tcaggcccag atcttcgttg agccggataa gcgtccgcag
     5401 cacatcctcg gccgattgcg ggtcgagttg gctcgtcggc tcatccagca ccagtacctt
     5461 cggccgctga accagagcgg cggcgatggc gagtcgctgg cgttccccac ccgacaatgt
     5521 ctgcacggcg cggccgcgca agggggtcag ctccagcaga tccagcacct cctccacgcg
     5581 aacgcgcatc tcttcgcgtg gcacggcggc attttccagc gaaaaggcaa tttcgctttc
     5641 aactcgatca aggacagtct gtgattcagg atcctgcgca acaaagccga ccgttcggct
     5701 aagcacgtgc ggcccggcgc taatcgcgtt tagtccatag acttcgactt ctcccttaat
     5761 ggcgccgcca ctgaaatgag gcaccagccc attcaggcag cgcaggagcg tagacttccc
     5821 cgagctggac gggccggcca ccagaacaaa ttccccatca gtcacgttcc agtcaagctc
     5881 acacagggca ggttttacgc cttgcggata ggtataggtg aggcggttta tcttaatcaa
     5941 gcgggtacct caatcgcagg ctttctgaag aggataatta tcgccggaac agctaatccc
     6001 agaatagctg ccccgatcca ggtatcaaac ggtggcgccg ttaaatcagg atagggcgaa
     6061 taattcagcg atctgcgtga caccaacaat atgccgagcg caatcagcgc accattgacc
     6121 acccaggtag ccgtcaggct ccagcgtcgg tcataatatt gcgtgcgtga cacctgtcgg
     6181 ctcaggtcaa gatacgcccc tgcgattgct aacccgccga tggcgatcag cgcccagcca
     6241 tcaagcccgc cccaaactag tcggagcgcc ccagccagtg ttaataacaa tcccgcaagc
     6301 atggcggctc ttgctcgcgc aggcatcgag acgttggtcg ttgagccata accgcgcgcc
     6361 accatcgtct cggacaggtt caacgcacgc tcgagcccgg agataagcaa tggaataagg
     6421 atcgggtgcc agattcgcca accacgcatc cgatagccgc gaatcgcctg cgcatcgcgg
     6481 atacgccgat attggccaat cgtctcaggg atataggtga tagcaatcaa caaaaccagc
     6541 cctatctcat gcaaggctcg cggcgtcagg ccagccaatt ggctggcagg aacgatcgaa
     6601 ttgaaggcca ggaagaatga cagcaacgtg atcagtctca acccgttcag aaagccaaag
     6661 acggccgctt ccagagtgat atcgcctccg attaaaggcc actgggcggg caacgcaaac
     6721 agcgaggttt gcccgaaatg cgcggttagt gcgttgaaaa gagtcgagaa gaaaagaatt
     6781 acaatcgcaa tacgccaaaa aggcagacgc cattgttgtg gtatgtgcga ggcgcaggcg
     6841 tactccacaa gccgggagat gattaggagc aagatcagat agaatgggtt gctggttagt
     6901 atcgccagca atccaccggc aaagagccag acgacccagg cgcgaacatc cagggtcatg
     6961 acgcctgcca tgcctgatac tgcattcgtc cgtgaaatcg agtcaacgcg cgcactgtcg
     7021 gcaacccaaa ggcagccatc aggatcacgt tgcccagagc agcggccgaa tcccaaacga
     7081 gcgaagtcgt cagataaaat gcggcgtagc ggcgaatgga atccccaacc ccgataccgg
     7141 ccgcccaggt ggtagtggcg tcaccgctga agtaaggcca tgtgtagaga ttcataatcg
     7201 ccccatagaa gaatccccag acgaacgcaa acgctatcag caaaaaaatc tcggccctgc
     7261 cgtgcgacgg atggggcaac agcccggctg tcaggccgat ccaaccggca gtgaacatct
     7321 gatagggcag ccacggccct accccggcgg tgataaccgc tgaaaccagc atcgtcatgg
     7381 ctcccaacag aaacccaaat cgcgcgccaa acacatatcc gccaagaatg attggcgcga
     7441 agattggcga gaagccgccc ggcccgggaa tgccgacttc gataaagcgc aatgtcgatg
     7501 tggcggcaac cataatgcca agcgccgcaa ccgtcttggc cccggccgcc tggccctgga
     7561 gttccaccaa caggacggcc aggcagagag aaacgagcaa ggtcgttagc aatggcgttg
     7621 cggtggtcga tgccgaaccg tctccaatgc ctaagggggc aaaggccact atcagaaacg
     7681 gatacgccag ggcgaggata ccgatcatcg cggcaaccac atagagcgcg aaggagaggg
     7741 ctttgcttct catcaaccat ttccgccggg gtgagtcatc tcgcggcgtc gatagatgag
     7801 caacgccaac gccgccaaca ggagtaatag cccgaaaaat ccaatgtagg aaatcccggg
     7861 cgatgattca tcaacttggg aagtagcggc agcgatcaca ggattatcga tagtagtgct
     7921 catcccgatg acggccgaca cggagatagg cgtcgaggtg ggcgtagcaa gcgatgttga
     7981 ttgattgctg tcaattaacc cacccccaat tccggtttcc gcaattgctt ccggcgtcga
     8041 cacaacgacc atctcaccac ctacaggctg tggtgccgtt atgattggcg catcaggttc
     8101 gggcgaggcg gcttccggca ggccacctcc tggttgaccg ggatatgact caccaccccc
     8161 caggctaggc ggcgcgacgg gggcaggtgt ttcacctgca tcgaaaggcg cctccggaat
     8221 gggcgtctct gtctgattgc cgatggcaat cggacccgat gtggggttgg cagttgtcgc
     8281 cattggcata gtcggcgaag cagctaccga aacagttgcc atggctatag atgtagtagc
     8341 cgtcgggttc ggagcattct ctgttggcgc gcgagtaggt aacaccatcg gagtcgatgt
     8401 aggtctgggc gtgctcgtgg ctgtcggaga ggcggcattc tcatcgatac atatctcatt
     8461 gaatggaata gcgggcgggg gcttggcctg atcgacagat cccaggcccc atacccagcc
     8521 atcaatcgcg ccatcctgta gcttgtaaat acccgacccg gcgacagagt agctccacgt
     8581 tccattgacg aaatgccaat aggaccagta tttgcattcc ccgccgccgc gacacgcaca
     8641 aaagcaatca tcagaggggc acccttgccc gtcgatacgg caaatagccg cgccgccagt
     8701 ctgaaaatct gtctcaacgg gcaaaccgga acgctgcaat gcctcatagc ccgtgatgct
     8761 gtcttcctgg aaactaacac acctggcggc aatttggcca ttgccaaaat cgaccaccag
     8821 cgcgacctga ttctcattgc cggcccagga gggatcaacg gccatgagaa tcacgatcag
     8881 gccggtgaga cacaggagaa gcaagaacca cgggcttttc aacgaaacaa catcctcacg
     8941 ataaaaattg tctgaatttc aatcgtgcta attttgcgtc atgatggcgg gcaaatgcac
     9001 cagggaaaac ccccattcag gttgagtcgc caacatcagg atggcatccg tagtcgagta
     9061 gggatcgtca cctgggccgt aggcgttggg ccaacaacca ttctgccctt gctggcccat
     9121 caccacgtcg gccgcgtttg ctaggcgacc atctatcacc aagctccatt gcgggccata
     9181 agggttttgc ccgataccgg ccaatccttg caccacctcc gaagttgcgc tcggatttga
     9241 tacgtcacca aaaccccaac ttccatccgt gagttgttcc gaatgaagga cagatagcgt
     9301 ggagatcggc gcgcgggaat ccagttcacc caggacctga agggcaatcc cgttggcgtc
     9361 cgagtcgccc caatcgccat tcgtctggga gcttaacagc agctcaatcg cggtgggagc
     9421 cacctcctca tcggccctca aaaggcccag catcgcctcg gcgtgggcga atatcccaaa
     9481 ggcggtggaa tcatattcac cggagggttg aagatggccc gccatcactt ccggcagatt
     9541 catgccggcg aagttcgcca catcatacgg cgatccggcg gcaaccacgc cctgcatgac
     9601 gatcccaacc cggccgcctg cccccgaaga cagatagtca ggagtcagcg cttccaacgc
     9661 ttcaacagca tttgtgtcgc cggatgtcgt ccagcgcacc gattggggat cgtctcccgc
     9721 agccgcaatc gcctgaatag ctcgcgacgt gcctgaggca tcggtcgatc cgccggcaaa
     9781 cgacggccaa ccgccactcg tcgtatcctg catcccgtcg aggcaagtca gggcggttaa
     9841 tgccgcctcc agccgggcgg gaaggggaaa cgcgcggccg ctcgcagccg gtatagcctg
     9901 aaccgttgcg aagaaatcat cgaacggccc ctggccaaag tcggattgga acgcaccgct
     9961 ccagctctgg aaagcgagca gcacctggag aggagaacgg ccattcatgg cccattcgcc
    10021 gccatcgtca taccacggct catccagcgc ggatagcgcc tgaatgacaa aagccgtcga
    10081 attggcgttt ggcgtaaatt cggcgctgta tccccagcca tcttcctgct gcgcgtcggc
    10141 caggaattgc acggccgatt gaatcgcact tgtccccggt tcacgccccg cagcaatcag
    10201 agccatgatg gccatagccg tggcatcggg gttattcgtc gtgccgaagc cgtcgtccca
    10261 cgcgccggaa gcgccttgtt tgctttccag ccagacaatc gccgattccg gcacagtctg
    10321 attgaccgac gccacaccca gcatcgccat agcctgctta aagggatcgc caactccata
    10381 ggcgccggac ggttccaggc tgttggtcag aatctctaca aaattacgat cttcgaagtc
    10441 ccgaggatcc acggcggacg cagtaagcgc cagaacaact ttgcccgcct gaccgccgtt
    10501 tgccgaggca aaggtttgca gggcggcgtc attgtcttcc agataatcca gtggggtggc
    10561 ttcctggcct ggatatcgag ccgccggatt atgaccggca gcggtgatgg ccaggatggc
    10621 atcaagagtg ccggggatgg tcgacggcgc ctggttggcc cccgtgctca aggaagcata
    10681 acccccatca tcattttgga actcgttgac catccagaat acggccgcct gaaccgacga
    10741 ccgctgatca gggaaagccg gcgcgcttgc agctt
//
LOCUS       DAOMQG010000086        10658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2664_length_10658_cov_9.990380, whole genome shotgun sequence.
ACCESSION   DAOMQG010000086 DAOMQG010000000
VERSION     DAOMQG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2664_length_10658_cov_9.990380"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLC06_06730"
     CDS             <1..198
                     /locus_tag="PLC06_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90175.1"
                     /translation="YANEAGKIVCFFQSAQKFGARYATLGFNDSANLDDGTFWPVAYG
                     LTALTADVEAKIRALVNQAVS"
     gene            431..2320
                     /locus_tag="PLC06_06735"
     CDS             431..2320
                     /locus_tag="PLC06_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRO90176.1"
                     /translation="MNFWTGLILGIIIGWLVEWIIDWLFWRRDAAEAQEAELLLAEGQ
                     AKSIDLNEDWGSRLADADLDYQERLRAVEGEWQGRLNLNEQQWQSQFAALEDENENLR
                     ARLAAGAALGTAGAALSSDTAKDEVLYSYALDDSIAIDDADAAAELAEEGEAIEIEFP
                     DYEESWEAEQMAIAPVEVETEPPDFSLLGEVDNDLAERLRLAGVDSIEALAAADPAAL
                     SVTTGLERAEAESWIARAVAVRAPEIDLAAQSRPLNYQDDLTRIRGIGPRYAALLGEA
                     GITTYDDLIAASPDRLRDIIKPSAMQQLNFDSWNRQAQALAETREARAGDDLTELEGI
                     GPVYASKLRDRGIATFADLAATDEATLNEIIGAPAWRKINYGEWIEQARLTASGDTIA
                     LEELKSRLFRRGGDNLSLIRGMGERSATALQAGGITSFSALAQSSPQQLDEIIRNAGV
                     RGGYDYEAWISEAELRAAGKRIPGTRSRAIQIVPCPQDLSAVSGIGPVYEERLYAAGI
                     GTYWSLAELPVEELARILELKSFQDVDMAAISTAAMNMAAETNSLGRGWDGSSPDDFD
                     GLPEIGEVYERRLYEAGICTYEAMAAATPERLAEICKAPPMRIPNYAAWIATAAELSA
                     AKRSG"
     gene            2324..4102
                     /locus_tag="PLC06_06740"
     CDS             2324..4102
                     /locus_tag="PLC06_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRO90177.1"
                     /translation="MARYHTAEEIRRYDLMKLGVLLLLILLLILTWFATRGRNDALLG
                     TGDEMAATPIAESSGAEAGAIVAPALSIPSVNLPAEPLQPGSVTLSGIAAPGAQVTVL
                     VNGVPAGMATAGVDGNWSATTELPAGSYTVQARIMDNVGGVAGESEAVSIDVGGVALP
                     AAPIAISPPGFDALAGTYTFIGTAAPGQLIQVVANGAVVGSAAADEAGNFAVEVPAAA
                     VGGEAQLQTVDATGAIVQQSEPLKLNSRPPSLAPSADMRADADRGTLILPSRPEGVTL
                     SGQGEPGTQVELLVNGTRGALAVVDAGGVWSTSLTLPDGTYTFQFNTLDPGGNLLSSA
                     TPVTVAVGEAAAAVIGAPAPEATAAAPAESANQTLADVLAGRPEFSTLLSVLQSTGSL
                     DVLAQPGPFTVFAPTNEAFANLPQRVIDGLNANPQILSQLLQYHIARGRHPVADLQTV
                     QPATVNGRLLTVGMLDGVTTINDALVTGADIPAANGIAHAIDRILVPPLVEGVRPPII
                     DASGVPTFTGTFLTIVGTAEPGRTILVELNGEPFGEAAVVGPDTTWAVSGNVTSGEYQ
                     IVAYMLGAADALEAISRPVMLQVNAQ"
     assembly_gap    4336..4435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4436..5059
                     /locus_tag="PLC06_06745"
     CDS             <4436..5059
                     /locus_tag="PLC06_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90178.1"
                     /translation="GAQQGGGAGDMAGMLGSLMGGGGAQQGGAGGLLGGLLGGLMGGG
                     GGNAGQMSFGGGGGGMSLPFAGTLAEKLGISEQMANMLIMGAIGLLTSSMAKNRSGGS
                     IDVNGLTDPDYIRSSGVAGRLSNQMGISEEDAILGLQQTMGMMMAEQGHKPAAKPKSG
                     TKKKKTTTAEAAPKKSSSKATKTTPKKKTTKKSSGPDFMDLLDDPKG"
     gene            5217..5594
                     /gene="mscL"
                     /locus_tag="PLC06_06750"
     CDS             5217..5594
                     /gene="mscL"
                     /locus_tag="PLC06_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRO90179.1"
                     /translation="MIKEFKEFMLRGNVMDLAVAVIIGGAFTAIVTSLVNDIIMPVVG
                     VILGGVDFTALAITVGDANITYGNFIQAIINFLIIALVLFLLIRSINSMSRKKEEAPA
                     APPEPTAEEKLLAEIRDLLRERR"
     gene            5697..6686
                     /locus_tag="PLC06_06755"
     CDS             5697..6686
                     /locus_tag="PLC06_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90180.1"
                     /translation="MSLFRRIIGIIMIIVGIVGLVISVAGAYFAGQAIDALGNGLNST
                     VTLLESTVDTTTASLVNVKATLGEASATLGTVTDATRNVATTIFDTQPLLEQATTMTT
                     DTLPTSLDAVNTAIPNLAGIAASIDTTLTTLSNFRINQTIGTGPFAIPLNFDLGIGYA
                     PEEPFDTAVLAIGESLVPIPDQLRELETNLTTMTTNLGNIGTDVEALSGNISGINTTV
                     EQFIPLIDQYITLLDQITGSLVTARDQINANIGMLKWVAIGLMLWFAVYQVMPIYIGY
                     RMLADKVVEGSVEEYLEDERKEMEERVKEAEEKAEDAEEKAEELADAARDADK"
     gene            6824..9061
                     /locus_tag="PLC06_06760"
     CDS             6824..9061
                     /locus_tag="PLC06_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRO90181.1"
                     /translation="MKQFTLRHRAGYLSIWGMFLIMFALVACRGAGQVTPQATPVEEP
                     AGGAPTTEIAATVPAPTLAATPANDGGATRPADALTTVRERGRLIVGTSADYPPFAFY
                     NDAFELDGYDIALARLIGEQLGVDVEFKDMAFDGLGGALQVGQIDAALAAISITDARR
                     ELIDFSTLYFISEGAALAQADAGITINQVADLAGYRVAVQSGTIYQAWVQDEAVDAGL
                     LPPDNLLVYTDAGKAIEDLRAGLVDVVIADRLPLELAASDPALAIVGEGLNRQRIAVA
                     LAKGSTLLAPINEALFELQSQGELARLAEEFLQLDQTELQPLPGPEEVVEEDATPTEQ
                     ARLTGACIDAMTLIAHLSFDDDGMRSPPPVSPGTPFQKTWRVQNNGTCTWTTGYVLTP
                     VGGNVAQATMGGAPTPIQAPVAPGQTTDITVNLVAPLLPGVYQGFWSMRGPGGLLFGD
                     RLWVGITVPTQPTPTPPPTATVSPSIEFAADRTTIRAGECVVFRWNVVNGGEVSFNAQ
                     GQSPALNRVAPAGSQTECPPATMFYELRVTMPDGSTEIRNIRVDVQPTLNAPIFESFS
                     VTPSQQITVGQCVDVRWRISGDVTNIRVARNDTTLWNGAPLSGTSRDCPPVGEAVYSI
                     EATGAGGTSRALENVTVLQATPAPGVPTATPAAGQLPVINSLIVSPNRIFVGSCLTVA
                     WNVSGNVSRVQLRRDGVLVLDFALFSGNVTDCLNNEGTYIYRLDAVNAQGGLVFQQAS
                     ATVTR"
     gene            9143..>10658
                     /locus_tag="PLC06_06765"
     CDS             9143..>10658
                     /locus_tag="PLC06_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRO90182.1"
                     /translation="MADNLEGRNRGFDDQPIYEDDTRAYDPEPPDQNYALDEIEAIDN
                     VESGLSWLLVGLVALAAVIVGLLIYYFLFNGGGGGSIIGPVETTVPGDDSWNRVRTSG
                     RLVVGTSLDYPPFAFRNAQFQPDGFDMAVITDVASRLGVATEIQDMAFDSLGSALNMG
                     QVDVAIAAITVTAAREEQVDFSNIYWIGKEGILARADATVPPITSLSGMAGWRIGVQR
                     GSVYQTWLQRDLVDSGMTQAGNLFVYETVDAAVRDLREQRLDLVVIDRRVAEAAVAQG
                     GVRIVGGGLNPQRYAIAMRNGAESLRVQINSALTQLQNEGRLAVLAQQYLGAGPGAII
                     TPPAPTATVPAQPTTPPTQPTAAPCVNGMRFVGDLNLDDNNMQSPPPISPGQPFSKGW
                     RIQNSGTCAWTTAFRLVYVSGNVPGASMGGQPTAISGFVAAGQQYDMFVNLVAPLTPG
                     VYQGIWQMVDDQNRPFGDRIWVGITVPAAPTATPPPTQTPSPSIQFTVDRNNILQ"
BASE COUNT         2029 a         3405 c         3271 g         1853 t
ORIGIN      
        1 tatgccaatg aggccggcaa gatcgtctgc ttcttccaaa gtgcgcaaaa gttcggcgcc
       61 aggtacgcga cgctgggctt caatgactcg gcgaacctcg acgacgggac cttctggccg
      121 gtcgcctatg ggctgacggc attgaccgcc gatgtagagg cgaaaattcg cgcgctcgtg
      181 aaccaagcgg ttagttgagg ccttgtcatc cccacggcct tcgccgggtt gataacgcat
      241 cccctctctc caaactttcc ccggccgggc ggccatttgt caaagtcgac aaatggccgc
      301 ccggttttta tttgccgccg gattctaaaa agatcattgt gtttatgttt accaggcgcg
      361 caattccggc aaaagcatat atacttatgc cagatggttg ttgggaagtt tgtcccatgg
      421 aggtgcgcat atgaacttct ggacgggtct gatactgggc atcattatcg gctggcttgt
      481 ggaatggatc atcgattggc tgttttggcg gcgggatgcc gcggaggcgc aagaagctga
      541 attgcttctc gccgaggggc aagccaaatc gatcgatctg aacgaagatt ggggatcgcg
      601 actggccgac gccgatctgg attatcagga gcgcttgcgc gccgtagaag gcgagtggca
      661 gggcaggctg aacctcaatg agcagcagtg gcaatcccaa ttcgcggcgc tggaagacga
      721 aaacgagaat ctgcgcgccc gactggctgc cggagcggcg ctgggtacgg cgggagccgc
      781 tctgtcctct gatacggcca aggacgaggt gctctattcg tatgcgttgg atgattccat
      841 cgcgatcgac gacgccgatg cagcggccga attggccgaa gaaggcgaag cgatcgagat
      901 cgagtttccc gattacgaag agtcgtggga ggcagagcag atggccattg caccggtcga
      961 ggtcgagacc gagccgcccg atttttcact gctgggcgag gtggataacg acctggccga
     1021 gcggctccgt ttggccggcg tcgattcgat cgaggccctg gcggcggccg accccgcagc
     1081 cctgtcggtg accaccggtc tggagcgcgc cgaagcggaa agctggatcg cgcgcgcggt
     1141 tgcggttcgc gcaccggaga tcgacctggc ggcccaatcc cgccccctca actatcagga
     1201 tgacctgacc cgcatccgcg gcattggccc ccgctatgcc gcccttctgg gcgaagcggg
     1261 catcaccacc tatgacgatc tgatcgccgc ctcgcccgac cggctgcgcg acatcatcaa
     1321 gcccagcgcc atgcagcagc tcaacttcga cagctggaat cgccaggccc aggcgctggc
     1381 cgagacgcgc gaagccagag ccggcgacga cctgaccgaa ctggaaggca tcggccccgt
     1441 gtatgcctcc aaattgcgcg accgcggcat tgccaccttt gccgacctgg ccgcaaccga
     1501 tgaggcgacg ctaaacgaga tcatcggcgc gccggcctgg cgcaagatca actacggcga
     1561 atggatcgag caggccaggc tgaccgcttc gggcgacacc atcgccctcg aagagctgaa
     1621 gagtcgcctc tttcgtcgtg gcggcgacaa cctgagcctg attcgcggca tgggcgagcg
     1681 ctccgcgacc gctctgcagg cgggcggcat cacctcgttt tccgcgctgg cccaatcatc
     1741 gccgcagcaa ctggatgaga tcattcgcaa tgccggcgtg cgcggcggct atgattacga
     1801 agcctggatc agcgaggctg aattgcgcgc cgcgggcaaa cgaatccccg gcacccggtc
     1861 gcgggccatc cagatcgtcc cctgtcccca ggacctctcg gccgtctccg gcatcggccc
     1921 cgtttacgaa gagcgtctct acgcggcggg gatcggcacc tactggtcgc tggccgagct
     1981 gcccgtcgag gagctggcgc gcattctcga actcaaatca ttccaggacg tggatatggc
     2041 cgccatctcg accgcggcga tgaatatggc tgcggagacc aactccctgg gtcgcggctg
     2101 ggacggctcg tcacccgatg atttcgacgg gctgccggag atcggcgaag tctacgagcg
     2161 tcgcctctac gaagccggca tctgcaccta cgaagcgatg gctgccgcta cgccggagcg
     2221 actggccgag atctgcaagg cgccgccgat gcgaatcccc aactacgccg cctggatcgc
     2281 tacggccgcg gagctctccg ccgccaaaag gagtggctaa gcgatggccc gctatcacac
     2341 cgccgaggag atacgccgct acgatttgat gaaattgggc gtgctgttgc tgctcatcct
     2401 gctattgatc ctgacctggt tcgccactcg cggccggaat gacgctttgt tgggcacggg
     2461 cgatgagatg gcagccacgc caatcgctga aagctcagga gccgaagcgg gggccatcgt
     2521 cgcgcccgcg ctgagcatcc cgtccgtcaa tctgcctgct gagccgctgc aaccgggcag
     2581 cgtgaccctc agcggcatcg ccgcccccgg cgctcaggtg accgtactgg tcaacggcgt
     2641 gcccgcgggc atggccaccg ccggcgtcga cggcaactgg tcggccacga cggaattgcc
     2701 ggccggcagc tatacggtcc aggcgcgaat catggacaat gtcggcggcg tggccggcga
     2761 gtcggaagcc gtctcgatcg atgtcggcgg cgtggcactc ccggcagcgc caatcgccat
     2821 cagcccgccg ggcttcgacg ccctggcggg aacttatacg ttcatcggca cggccgcccc
     2881 cggccagctg atccaggtgg tcgccaatgg cgccgtcgtg ggcagcgccg cggccgacga
     2941 ggcgggcaat ttcgccgtcg aggtgccggc ggccgctgtc ggtggtgagg cgcaattgca
     3001 gacagtcgat gcgaccggtg cgatagtcca gcaatccgag ccgttgaaat tgaattcgcg
     3061 cccgcccagt ctcgcgccat cggccgacat gcgggcggat gccgatagag gcacgctcat
     3121 cctgccctcg cggccggaag gcgtgactct gagcggccag ggcgagccag gcacgcaagt
     3181 ggagttgctg gtcaatggca ctcgcggcgc gctggccgtg gttgacgccg gcggagtctg
     3241 gtcaacgtca ctcacgctgc ccgacggcac gtatacgttc cagttcaaca cgcttgatcc
     3301 cggcggcaat ctcctttcga gtgcgacgcc cgtcaccgtc gccgttggcg aggccgcggc
     3361 ggccgtcatc ggcgcgcccg cgccggaggc aacggccgcc gccccggccg aaagcgccaa
     3421 tcagaccctg gccgatgtgc tggccgggcg ccctgagttc tccaccctgc tctccgtcct
     3481 gcaatcgaca ggctcgctgg atgtcctggc ccagcccggc cccttcaccg tcttcgcgcc
     3541 gaccaatgaa gcgttcgcga atctgccgca acgggtcatc gacgggctga acgccaaccc
     3601 gcaaatcctg tcgcagctgt tgcaatatca catcgcgcgc ggtcgccatc cggtcgcgga
     3661 cttgcaaacg gtgcaacccg ccacggtgaa cggccggttg ctgaccgtcg gcatgctgga
     3721 cggcgtaacg accatcaacg acgccctggt gacgggggcc gacatcccgg cggccaacgg
     3781 cattgcccac gccatcgacc gcatcctcgt cccgccgctg gtcgagggcg tgcggccgcc
     3841 gatcatcgac gcgtccggcg tgccgacttt caccggcacg ttcctgacga tcgtcggcac
     3901 ggccgaaccg ggcagaacga ttctggtgga gctgaacggc gagccgtttg gcgaagccgc
     3961 cgttgtaggc ccggatacaa catgggccgt cagcggcaat gtgacgtcgg gtgaatatca
     4021 gatcgtggcc tacatgctgg gcgcggccga tgcgctggag gccatctccc gcccggttat
     4081 gttacaggtc aacgctcaat gaggtatagt tgaccgggga gagtgagcgg agactcatcc
     4141 ccggcataga ctgacaggtc attatatttt gatgggttca ggcaaagcag ggcattcgcc
     4201 cacaacgccg ccggtttgtg ccgcagcgtg aaacgataag ggagaaaaaa tgagcgataa
     4261 ttttatggat ttgctgtcag gcgaaggccc cgacaagaaa ggacagggag accgcggtat
     4321 ggaggatctc ctcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgc
     4441 tcaacagggc ggcggcgcgg gcgacatggc cggcatgctc ggtagtttga tgggcggtgg
     4501 cggcgctcag cagggcggcg cgggtggctt gttgggcggt cttctgggcg gcctgatggg
     4561 cggtggcggt ggtaacgccg ggcagatgtc ctttggcggg ggcggcggcg gcatgtcgtt
     4621 gcccttcgcg gggacgttgg ctgaaaaact gggcatctcc gagcaaatgg ccaacatgct
     4681 tatcatgggg gccatcggcc tgctgacctc gtccatggcc aaaaaccgca gcggtggctc
     4741 gatcgatgtg aacggcctga ccgatcccga ttatatccgc tccagcggcg tcgccggccg
     4801 gctttccaat cagatgggca tcagcgaaga ggatgcgatc ctgggcctgc aacagaccat
     4861 gggcatgatg atggccgagc aagggcacaa accggccgcc aagcccaaga gcggcaccaa
     4921 aaagaaaaag acgacaacgg cagaagccgc ccccaagaaa agctcgtcca aggcaaccaa
     4981 aaccacgccg aagaaaaaga cgaccaagaa aagctccggc cctgatttta tggatttgct
     5041 ggacgacccg aaaggttaat cgtgatagcc ctggagaacg caactattgc atattaacaa
     5101 gctgtagggg cgggtgcgac aggcggcctg tcgtccaaac ccgcccctgc acaccaacgg
     5161 gtgactgtga tacgaactta agcaatcttg cgtaacagtc tatttacgga ggaattatga
     5221 tcaaggaatt taaagagttt atgctgcgcg gcaacgtgat ggatctggct gtcgccgtga
     5281 tcatcggcgg cgctttcacc gccatcgtga cttcactcgt caacgacatc attatgcccg
     5341 tcgttggcgt catcctcggc ggcgtcgatt tcacggccct ggcgatcaca gtaggcgacg
     5401 cgaacatcac ttatggcaat ttcattcaag ccatcatcaa ctttttgatc atcgcgctcg
     5461 tcctgttcct gctcattcgc tcgatcaata gcatgagccg caagaaggaa gaggccccgg
     5521 ccgcgccgcc cgaaccgacg gccgaggaga aactgttggc cgagattcgc gacttgctgc
     5581 gcgaacgtcg ttaaacattg aatcagccgg cgcgctatcc ttcatggatg gcgcgctgct
     5641 tattggcaac ttcatcgcaa tcgcccccac gggcataatt tccggaggaa gaagagatgt
     5701 cattatttcg tcgaatcatc gggatcatca tgatcattgt cggcatcgtc ggtctcgtca
     5761 tctcggtcgc gggcgcctat ttcgccgggc aagcaattga cgccctcggc aatggcctca
     5821 actcgaccgt caccttgctt gagagcaccg tcgacacgac tacggccagc ctggtaaacg
     5881 tcaaggctac cctgggcgag gcgagcgcta cgctgggcac cgtcaccgac gcaacccgca
     5941 acgtggccac caccatcttc gacacccagc cgctgctgga acaggcaacg acgatgacca
     6001 ccgacacgtt gcccaccagc ctggatgcag tcaacacggc tatccccaac ctggcgggga
     6061 tcgccgcctc gatcgacacg accctgacaa cgttgagcaa cttccggatt aaccaaacaa
     6121 tcggcaccgg tccgtttgcc ataccgctca acttcgatct cggcatcgga tacgcgccgg
     6181 aggagccgtt tgacacggcc gtgctggcta tcggcgaaag cctggtgccc atccccgacc
     6241 aactccgcga gctggaaacc aacctgacca cgatgacgac caatctcggc aacatcggca
     6301 ccgacgtcga ggcgctgtcc ggcaacatct ccggcatcaa caccaccgtc gagcaattca
     6361 tccccttgat cgatcagtac atcaccttac tcgatcaaat taccggctcg ctggtgacgg
     6421 ctcgcgatca gatcaatgcc aacatcggca tgctcaaatg ggtagccatt ggcctcatgc
     6481 tctggttcgc cgtctatcag gtcatgccca tctacatcgg ctaccgcatg ctggccgaca
     6541 aggtggtgga aggcagcgtc gaggaatatc ttgaggacga gcgcaaagag atggaagagc
     6601 gcgtgaagga agccgaagaa aaagcggagg atgccgagga gaaggccgaa gagctggccg
     6661 atgccgctcg ggatgcggat aagtaagccg caaggcttca tataagaaag cgcaacgacg
     6721 cgggcggtcg ccgggccaag ggccggcggc cgccgggatg cctttagcgt cgaccggcag
     6781 gggatgcagc tcgcgcattt cgggatcgat atgagtagtg gccatgaaac aattcactct
     6841 gagacaccgc gccggctatc tgtccatctg gggcatgttc ctgatcatgt ttgcccttgt
     6901 ggcctgtcgc ggcgcgggtc aggtgacgcc acaagccacg ccagttgaag aacctgccgg
     6961 tggcgcaccg acgacggaga ttgcagccac tgttccggcc ccgacactcg ccgccacccc
     7021 ggccaacgac ggcggcgcaa cacggccggc cgacgccctg accaccgtgc gcgagcgggg
     7081 acggctgatc gtcggcacat cggccgatta cccgcccttc gccttctata acgacgcctt
     7141 tgaactcgac ggctacgata tcgccctggc ccgtctgatc ggcgagcagc tcggcgtgga
     7201 tgtcgagttc aaggacatgg ccttcgacgg cctcggcggc gcgctgcagg tggggcagat
     7261 cgacgccgcg ctggccgcca tctcgatcac cgacgcgcgg cgcgaactga tcgacttctc
     7321 caccctctac ttcatcagcg agggcgcggc cctggcccag gccgacgccg gcatcaccat
     7381 caatcaggtc gctgacctgg ccggctatcg cgttgctgtc cagagcggga ccatttatca
     7441 ggcctgggta caggatgaag cagtagatgc cggattgttg ccgcccgaca acctgctggt
     7501 ctataccgac gccggcaagg ccatcgagga tctccgcgcc gggctggtcg atgtggtcat
     7561 tgccgatcga ctccctctgg agctggccgc gagtgacccg gccctggcca tcgtcggcga
     7621 agggttgaat cgccagcgca tcgccgtcgc tctggccaag ggttccacct tgcttgcgcc
     7681 gatcaacgaa gctctcttcg aactacagag ccaaggcgaa ctcgcccgac tggcggagga
     7741 gtttctgcag ctcgaccaga ctgagttgca accgctgccg gggccggaag aggtcgtcga
     7801 ggaagatgca acgcccaccg aacaagcccg tctcaccggc gcgtgcatcg acgccatgac
     7861 cctcatcgcc catctctcgt tcgacgatga cggcatgcgc tcgccgccgc cggtcagccc
     7921 gggcacgccg ttccagaaga cgtggcgcgt ccagaacaac ggcacttgca cctggaccac
     7981 cggctatgtg ctgacgcccg tcggcggtaa tgtggcgcaa gcgaccatgg gcggtgcccc
     8041 cactcctatt caggcccccg tcgctccggg acagacgacc gacatcaccg tcaatctggt
     8101 tgcaccgctg ctgcccggcg tctatcaggg cttttggtcc atgcgcggcc ccggcggcct
     8161 gctcttcggc gaccggctgt gggtcggcat caccgtgccc acccaaccta cgcccacccc
     8221 gccccccacg gcgaccgttt cgcccagcat cgagttcgcg gccgaccgaa caacgattcg
     8281 cgccggagaa tgcgtcgtgt ttcgctggaa tgtggtcaac ggcggcgagg tctccttcaa
     8341 cgctcagggc cagtcgcccg ccctcaatcg tgtcgcccct gccggcagcc agaccgaatg
     8401 cccgccggcc acaatgttct acgagctgcg ggtgacaatg cccgatggct caacggaaat
     8461 ccgcaatatt cgcgttgacg tccagccaac cctgaacgct cccatcttcg aatccttctc
     8521 ggtgacgccc agccagcaaa tcaccgtcgg acagtgcgtc gatgtgcgct ggcgaattag
     8581 cggggacgtg accaacattc gcgttgcccg caacgacacg acgctctgga acggcgcgcc
     8641 gctcagcggc acctcgcgcg attgcccgcc ggttggcgaa gcggtctact ccatcgaggc
     8701 gaccggcgcc ggcggtacct cccgcgcgct ggaaaacgtg actgtgttgc aggccacacc
     8761 cgcgcccggt gtcccgaccg cgacgccagc cgcgggccag ctgccagtga tcaacagcct
     8821 gatcgttagc cccaaccgca tattcgtcgg cagctgcctg accgtcgcct ggaacgtcag
     8881 cggcaatgtg agccgcgtcc agttgcgccg ggacggcgtt ctggttctcg attttgctct
     8941 cttcagcggc aacgtgaccg attgtctgaa caacgaaggt acctatatct accggctgga
     9001 tgcggtcaac gcgcagggcg ggctggtatt ccagcaagcc tcggccaccg tcacgcgcta
     9061 gagagagtgg caccggcctt gaattgccta ttgtcacccg cctcaaggtg acataaaata
     9121 ttcagggcaa ggaggtttca gcatggcaga taacctggaa ggcagaaatc ggggtttcga
     9181 cgaccaaccc atctatgaag atgatacacg cgcctatgat cccgaaccgc cggatcagaa
     9241 ctacgctctg gatgaaatag aagcgatcga caatgtggaa agcggcctga gctggctgct
     9301 ggtcgggttg gtggcgcttg ccgccgtgat cgtcggcctg ctcatctact acttcctttt
     9361 caacggcggt ggcggtggct ccatcattgg gccggtggaa acgactgtgc cgggcgatga
     9421 ttcctggaac cgcgtgcgca cctccggccg gctcgtcgtg ggcacctctc tcgactaccc
     9481 gccatttgcg tttcgcaacg cgcagttcca gcccgacggc ttcgacatgg ctgtcatcac
     9541 cgatgtggcc agccggttgg gtgtggccac ggaaatccag gatatggctt tcgatagcct
     9601 gggcagcgcc ctgaatatgg ggcaggtcga tgtggccatc gccgccatca ccgtgaccgc
     9661 ggctcgtgag gagcaggtcg acttcagcaa tatctactgg atcggcaagg aaggtattct
     9721 ggcacgcgcc gatgccacag tgccacccat caccagcctc tccggcatgg ccggctggcg
     9781 catcggcgtg cagcgcggca gcgtctacca gacctggctc cagcgcgacc tggtggactc
     9841 gggcatgacg caggcgggta acctgttcgt gtacgagacg gtcgatgctg ccgtgcgcga
     9901 tttgcgcgag cagcgtctgg acctggtcgt catcgaccgt cgcgtggcgg aagctgccgt
     9961 cgcccagggt ggcgtccgga tcgtcggcgg cgggttgaac ccgcaacgct acgcgattgc
    10021 catgcgcaac ggcgcggaat ccttgcgcgt gcagatcaat tcggcgctga cccagctaca
    10081 aaacgaaggg cgactcgcgg tgctggccca gcaatacctc ggcgcggggc cgggcgcgat
    10141 catcacgccc cccgctccca cggctaccgt ccccgcccaa ccgacgacgc ctcccactca
    10201 acctacggcc gcgccctgtg tcaacggtat gcggttcgtt ggcgatctga atctcgacga
    10261 caacaacatg caatcgccgc cgcccatcag ccccgggcag ccgttcagca agggctggcg
    10321 catccagaac agcggcacct gcgcctggac gacggccttc cggctggttt atgtgagcgg
    10381 gaatgtgccg ggagccagca tgggcggtca acccacggct atttccggct tcgtggctgc
    10441 cgggcagcag tacgacatgt ttgtgaacct ggtcgccccg ctgacgcccg gcgtctatca
    10501 gggcatctgg cagatggtgg acgaccagaa ccgaccgttt ggcgaccgca tctgggtcgg
    10561 catcaccgtg ccggccgcgc ccacggccac cccgccacca acgcaaacgc cgtcgccgag
    10621 catccagttc accgtcgacc gcaacaacat cctgcaag
//
LOCUS       DAOMQG010000087        10629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2673_length_10629_cov_10.762531, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000087 DAOMQG010000000
VERSION     DAOMQG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_10629_cov_10.762531"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /gene="fdhF"
                     /locus_tag="PLC06_06770"
     CDS             complement(<1..1745)
                     /gene="fdhF"
                     /locus_tag="PLC06_06770"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRO90183.1"
                     /translation="MIVPDRIVRTTCPYCGVGCQLNLKIKDEFIYAVEAPFDAAPNYG
                     MLCVKGRYGTDYVKHPGRVKTPLIRTNRHEGRSAPPVWREATWDEALDLTADELVRVA
                     RTYGGDAIAGFASAKATNEDNYVYQKLIRALFETNNIDHCARLCHAGSVAGLQMSLGT
                     SAMSNSIAEMENLEVFIVTGSNTTETHPVIANFLKRAVRQNGAKLIVVDPRRIDLVNF
                     STLWLQQSPGTDVAVFQAMAHVIVKEGLYNPNFIGERTEGFNNYIESLDEYTPEWAES
                     ISGVPAESIREAARIYANAGRAAIYWGMGISQSTHGSDNTLSLVNLALMCGHVGRSGT
                     GLNPLRGQNNVQGCSDVGALPNVYTAYQPVSDPAIRERFEKEWSKSLNPVPGLTATEI
                     IDASLSGGVRAMFIMGENPMMSEPNLNHTRHSLEQLEFMVCQDIFINETGVMADVFLP
                     ATSFAEKDGTFTNTDRRVQRCHAAVAPVGNSRPDWELLSDLGRRIEARLGLEQSAGFD
                     YSHPSDVWEEMRRVTPEFYGITYERIEREGGVHWPCPDLDHPGTPFLFADNFPRGKGK
                     FWQVQYGTVSEQPDA"
     gene            complement(1761..1952)
                     /locus_tag="PLC06_06775"
     CDS             complement(1761..1952)
                     /locus_tag="PLC06_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRO90184.1"
                     /translation="MQILENLGFVEVRRKGSHAQFRHADGRATTVPFHTGRDIAPTLL
                     RQIVKDINLTMDEFLAKRQ"
     gene            complement(1998..2207)
                     /locus_tag="PLC06_06780"
     CDS             complement(1998..2207)
                     /locus_tag="PLC06_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRO90185.1"
                     /translation="MATYVAVIERDIDTGYYVGYVPNFPGAHSQAETLDELYDNLKEV
                     IELLIEDGEPTLEAEFVGTQLIAIP"
     gene            complement(2330..2926)
                     /locus_tag="PLC06_06785"
     CDS             complement(2330..2926)
                     /locus_tag="PLC06_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015118522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO90186.1"
                     /translation="MIAEPVEQIETVELTRRRFTVAEYMRMAEVGLLDEDSRIELIRG
                     EIVEMSPINVLHALCVNRLTMLLSAKLTGRVIVSVQNPLQIDEYSIPQPDIALWKLPF
                     ENYADRLPGPPDVLLVIEVADSSIQYDRKTKTKLYGAAGITDYWIVNLAERQIEVYRE
                     PQTDGYRVTTRYKPGETLSPLAFADVNLGTDEILGPND"
     gene            complement(2993..>3535)
                     /locus_tag="PLC06_06790"
     CDS             complement(2993..>3535)
                     /locus_tag="PLC06_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRO90187.1"
                     /translation="RRLQAAACVTPVAEGMVVNTQTKDVRTARRVNLEMLNSAVDLSE
                     APHLLDLLQEYDVDTERFAGMDRRESPVHDDNPFYLRDYNKCINCWRCVQVCAQDAQY
                     TFALNFDNRGFHTLIGTFNDNGMTDTTCVFCGMCVAVCPTNALKPKRQFLLEQGIDPD
                     DILRQTRRGGKRRKQSVSEE"
     assembly_gap    3536..3635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3636..3858)
                     /locus_tag="PLC06_06795"
     CDS             complement(<3636..3858)
                     /locus_tag="PLC06_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRO90188.1"
                     /translation="MTSMVNLTIDGLPATVPAGTSILNAAAAIGIEIPVVCYHPHLTA
                     NGLCRICSVDIGRRLQAAACVTPVAEGMVV"
     gene            complement(4005..4859)
                     /gene="fdhD"
                     /locus_tag="PLC06_06800"
     CDS             complement(4005..4859)
                     /gene="fdhD"
                     /locus_tag="PLC06_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRO90189.1"
                     /translation="MNDGQRPPVDDRETPVGVRPAHYISLNGGDPLVVDGAVIDEVSA
                     CVSVNGMELTTFMCTPRDLTELALGFLYNEGVINRLDDVRAHHVSKSNSCVDVWLHDT
                     TFVRPRRAIVTAGCGGGLTFDDLSVAHGPLASNAHATPKQLADLMRQLHLGAELYQTA
                     RGVHAAMLAEIDANGEAQPLIQIEDIGRHNCLDKLQGAALLVGIPTKDRILLSSGRIS
                     SEMINKARRLETPIVCSRTSPTSLSVALAEAWNITIVAYLRQDRMRVYTHPERVISET
                     VFSRSVDQ"
     gene            complement(4856..6613)
                     /locus_tag="PLC06_06805"
     CDS             complement(4856..6613)
                     /locus_tag="PLC06_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRO90190.1"
                     /translation="MVQPATIDMTALEPILNRYRGRGRESLLPLLHEAQTIHGWLPPE
                     MQEAIGETLRVPLSDIHGVVEFYSMFYNEPMGKRVIRVCNDPACHMAGGPAVRLAIEE
                     RLGLKPGETSEDGLIGYETVPCLGMCEHAPNALNGDRPAGDLTPADVDAFLAGTYPEP
                     EPRIYGGPLVKLARAGKIDPTSLTDFVKHDGYKALRKALTMTPEEILAMMKGSDVLGR
                     GGAMFPVGLKWEMTRNAPGLPSEKHLVANADESEPGTFKDRALMEQDPFSLIESMTVA
                     AYTIGAENGWIFLRGEYPRCYKRLQNAIEKAREAGYLGRNILGRRGFNFDIELRLGAG
                     AYICGEETALFEAIEGKRGFPRIKPPFPTTHGLFQQPTAVNNIETLAATVAVLNMGVE
                     QWHKLGTAGSPGTKWFCVSGRVLRPGVYEVPFGLTVRQLIQMAGGVVGKEVQAVLLGG
                     ASGVFIGPRRLDTPLTYEDSKAQGFPLGSGVIMVFDEAVDLREIMYMLARFFAHESCG
                     KCYPCALGTQRQMEITERILHHGGPLANDRVDLKEIGLAMTQTSLCGLGQTAGLAVMS
                     AIDRWPELFEPDGGSGRAR"
     gene            complement(6965..7930)
                     /locus_tag="PLC06_06810"
     CDS             complement(6965..7930)
                     /locus_tag="PLC06_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO90191.1"
                     /translation="MSDSTHRYTLALEIGEEAIRQGKWQQALTCFQTALTGLPREPRV
                     YNGLGDTHTALSDRGRALACYKEAARLAGNNAEYVNKVAAAQEHLGMIPDAARSLLIS
                     GDMFWNQGQFDRAEAQWAYAVALRADFIAARERLALAARQRGDMDAAAGHFLDLADIL
                     RGDGRCLMALHICYTVMSDRPGDARVWAATNQAWRCVALRDRREDHRDARIESGDLIN
                     AAAEFAQWQLAAEIRQSAHANSSSPNPEAFIFLRQAMLNEGYGRAGVSISFYEKTIAA
                     GLTPPAIFFTLGLIYRLVGRKQDARAAHLLAARHPFYRNAVALLE"
     gene            complement(7927..8586)
                     /locus_tag="PLC06_06815"
     CDS             complement(7927..8586)
                     /locus_tag="PLC06_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRO90192.1"
                     /translation="MLSDWEPRFEQFIEHAMEETPDPAHDREHVRRVVANARQLAAAE
                     GADPAVVVPAAWLHDCVVVPKDSVHRARASQMAAERAGAFLREAGYPAEYIPAIEHAI
                     AAHSFSAGIPPQTREAQVVQDADRLDALGAIGIARTLLLGGMMGKPLYDHDEPLPKNR
                     APNDKENVIDHFYVKLLGLAAAMHTAAARREGERRTRLMRDYLQELAREISGDSLASA
                     S"
     gene            8748..9017
                     /locus_tag="PLC06_06820"
     CDS             8748..9017
                     /locus_tag="PLC06_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRO90193.1"
                     /translation="MKTQERRSRKVTISLPEELVVYTDRRADRKGTNRSQVISQALAH
                     LMAFEEEQLAAEGYAFYADEAEEFASSSDQAIAEAWGRDTEEAQS"
     gene            9014..9388
                     /locus_tag="PLC06_06825"
     CDS             9014..9388
                     /locus_tag="PLC06_06825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRO90194.1"
                     /translation="MIRRGEIYWVEFDPVKGSEQGGLRPALVIQNDTGNRYSPTTVVA
                     AITRTVPPKPYPFTVIIEPHESGLQATSAINCSQVATIQQSGPQSRLRAIPGENGIRP
                     IGRLSDAKMQEVSEALRFNFGL"
     gene            complement(9416..>10629)
                     /locus_tag="PLC06_06830"
     CDS             complement(9416..>10629)
                     /locus_tag="PLC06_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRO90195.1"
                     /translation="TDQRPITILCLASEFKGVPFIEECARQGARVILLTTEKYGSEPW
                     PWEAIYRHYNMPDLHTQPGITHAVSYLARENQIDRIVALDDYDVATAASLREHMRLPG
                     IGETQARYFRDKLSMRVQAASHGIRVPAFTSAFNYDQLRNYMAAISPPWVLKPRFEAG
                     AVGIRKLQDSEGVWRALDELGDGQSFYLLEQFVPGDVYHVDSLLWDGEVVFTVSSRYG
                     APPLTVTQGGGIFNTRLLPRDSEEFHETTNLAADVFRAFGLNQGVTHTEFIRAHHDGQ
                     YYFLETAARVGGAHIDKMVEAATGIALWQEAARIELAAERGEAYELPEHRNDYAGLII
                     CLAREQWPNLSAYDEPEIVWRVPREYHAGLLVASDNPDRVSQLLDEYNERFVRDFLMH
                     TPNKQGARTTF"
BASE COUNT         1983 a         3169 c         3283 g         2094 t
ORIGIN      
        1 tcggcgtcgg gctgctcgga gacggtgcca tattgcacct gccagaactt gcccttgccg
       61 cgcgggaagt tgtcggcaaa gaggaagggc gtgccggggt ggtcgaggtc ggggcagggc
      121 caatggacgc cgccttcgcg ctcaatgcgc tcataggtga taccatagaa ttcaggcgtc
      181 acgcggcgca tctcttccca gacgtcgctg gggtgactgt aatcgaatcc ggccgattgc
      241 tcgagaccca ggcgcgcctc aatgcgccgg ccgagatcgc tgaggagttc ccagtcgggg
      301 cgcgaattcc ccaccggcgc cacggccgcg tgacagcgct ggacgcgccg gtcggtgttg
      361 gtgaaggtgc cgtccttctc ggcgaagctg gtggcaggca ggaaaacgtc ggccatcacc
      421 cccgtttcgt tgatgaagat gtcctggcaa accatgaact ccagctgctc cagcgagtgg
      481 cgggtgtgat tcaggttcgg ttcgctcatc atggggttct cgcccatgat aaacatggcg
      541 cgcaccccgc cgctgagcga cgcgtcgatg atctccgtgg ccgtcaggcc gggcaccggg
      601 ttgagcgact tgctccattc tttctcgaac cgctcgcgga tggccgggtc gctgacaggc
      661 tgataggcgg tgtagacgtt aggcagcgcg ccaacatcgg agcagccctg gacgttgttc
      721 tggccgcgca atgggttgag gcccgtgccc gaacggccga cgtggccgca catgagggcc
      781 aggttgacca gcgacagggt gttgtccgag ccgtgggtgc tctggctgat gcccatgccc
      841 cagtagatgg ccgcgcgtcc ggcgttggcg taaatgcgcg ccgcctcacg gatcgattcg
      901 gccgggacac cgctgattga ctcggcccac tccggggtgt actcatccag cgattcgata
      961 tagttgttga agccctcggt gcgctcgcca atgaagttgg gattgtacag cccttccttg
     1021 acgatgacgt gggccatagc ctggaagacg gccacgtcgg tgccggggct ctgttgcagc
     1081 cagagggtag agaagttgac cagatcgatc cgccgcggat cgacgacgat gagcttggcc
     1141 ccattctgcc gcacggcgcg cttcaggaag ttggcaataa ccgggtgggt ctcggtggtg
     1201 ttgctgccgg tgacgataaa cacttccagg ttctccatct cggcgatgga gttggacatg
     1261 gcgctggtgc caagggacat ttgcaggccg gccacggaac cggcatgaca cagccgggcg
     1321 caatggtcga tattgttggt ctcgaacaag gcgcggatga gcttctggta gacgtagttg
     1381 tcctcgttag tggccttggc gctggcgaaa ccggcgatgg cgtcgccgcc ataggttcgg
     1441 gcgacgcgca ccagttcgtc ggccgtcagg tcgagcgctt cgtcccacgt ggcctcgcgc
     1501 cagacgggcg gggcgctgcg gccctcgtgc cggttggtac ggatgagggg cgtcttcacg
     1561 cgaccgggat gcttgacata atcagtcccg taccggccct tgacgcaaag catcccgtaa
     1621 ttcggggcgg cgtcgaacgg tgcttcgacg gcatagataa actcatcctt gatcttcaga
     1681 ttcaattgac agcccacccc gcagtagggg caagtcgtgc gtacaattcg gtcggggacg
     1741 atcatggtcg gttctcctgt tcactgccgt ttagccagga attcatccat cgtgaggttg
     1801 atatctttca cgatttgccg aagcagcgtt ggggcgatgt cacgcccagt gtggaacggt
     1861 acagtggtcg ctcggccatc agcatgcctg aactgggcgt gcgatccttt tcgtcggact
     1921 tcaacaaatc cgaggttctc aagaatttgc ataacttctc gcggtttaag aatcggaact
     1981 ttctgtccca ttctatttta tggtatagcg atcaattgtg tgccgacgaa ctcagcctca
     2041 agcgtgggtt cgccgtcttc gattagcaat tcaatcactt ctttcaggtt gtcgtataat
     2101 tcatcaagcg tttccgcctg agaatgcgcg ccggggaaat taggcacata acccacgtaa
     2161 taacctgtat cgatatcgcg ttcaatcact gctacgtaag ttgccatgta atcaccatcc
     2221 tttgacatca agcttggtgt cgggccccaa atccagccct cactgaacca gattgatgat
     2281 ctccttgaag cggtcgagcg tcggcttgtc catgacacat gtccgaatgt caatcgtttg
     2341 gcccaagaat ctcgtccgtg ccaaggttca catcggcaaa ggccagcggc gacaacgttt
     2401 cgcccggctt ataacgggtt gtaacacggt agccgtccgt ctgcggctcg cggtagactt
     2461 cgatttgccg ctcagccagg ttgacgatcc agtagtcggt gatcccggct gctccataga
     2521 gcttcgtctt tgttttccgg tcatattgga tggatgaatc ggcgacttcg atcactaata
     2581 gaacatcggg cgggccgggc aacctgtcag cgtagttttc aaaaggtagt ttccagaggg
     2641 ctatatccgg ctgaggaata gagtattcgt cgatctggag gggattctgc acgctgacga
     2701 tcacccgacc ggttagtttc gccgacaaaa gcatcgtcaa tctgttaacg cataaggcgt
     2761 gcagcacgtt aatgggactc atctcaacaa tctccccccg aattagctct atccggctat
     2821 cctcatctaa caggcctacc tcagccatac gcatgtattc cgcaacggta aatcgtcggc
     2881 gggtcagttc aaccgtctcg atctgctcaa caggttcagc gatcatgagc cctcgcttgc
     2941 cgcgatcggg ggagtgtcgt cgcggctttg ccgtattata caacatggga ggtcattctt
     3001 cggatacgct ctgcttccgt cgcttcccgc cgcgtcgagt ttggcgcaag atgtcgtcgg
     3061 gatcgatgcc ctgctccagc aaaaattgcc gcttgggctt cagggcgttg gtcgggcaga
     3121 cagcgacgca catgccgcaa aacacacagg tagtgtccgt catgccgttg tcgttgaaag
     3181 taccgatcag cgtatggaag ccacgattgt cgaagttgag cgcgaaggta tattgcgcgt
     3241 cctgagcgca gacctgcacg cagcgccagc agttgatgca tttgttgtag tcgcgcaggt
     3301 agaacggatt gtcatcatgc acgggcgatt cgcgccgatc catccctgcg aaccgctccg
     3361 tatcaacgtc gtattcctgc agcaggtcca gcaggtgggg cgcttcgctc agatcgacgg
     3421 ccgagttgag catctccagg ttcacacgcc gggctgtgcg gacatccttc gtctgcgtat
     3481 tgaccaccat cccttcggcc acgggcgtga cgcaggcggc cgcctgcagc cgccgnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgacc accatccctt cggccacggg
     3661 cgtgacgcag gcggccgcct gcagccgccg accaatgtcc actgagcaga tgcggcagag
     3721 gccattagcc gtcaggtgcg gatggtagca cacgaccggg atctcgatgc cgatcgccgc
     3781 tgccgcgttc aggatgctcg tccccgccgg cacagtcgcc ggcaggccgt cgatggtcag
     3841 atttaccatg cttgtcatac gacgtactcc gttctcgata atcagtattc agtaaggcag
     3901 tattcagtaa ggcagtagat cagtaggtca gtaggtcagt aggtcagtac ggagcctcga
     3961 ctgaatacgg atgcactgat ctactggtct actgatccac tgacctactg atccactgat
     4021 ctactgaaca ctgtttcact gatcacccgc tccgggtgcg tatacacccg catccgatcc
     4081 tgtcgcaaat aggccacgat cgtgatattc cacgcctcgg ccagcgccac ggacaggctg
     4141 gtgggcgacg tgcgcgaaca gacgataggc gtctccaggc gacgggcctt gttgatcatc
     4201 tcgctactga tgcggccgct gctgagcagg atgcgatcct ttgtcggaat gcccaccagc
     4261 agcgccgccc cttgcaattt gtcgaggcag ttatgacggc cgatatcctc gatctggatc
     4321 agcggttgcg cctcgccgtt ggcgtcgatc tcggccaaca tggcagcatg gacgccgcgc
     4381 gccgtctgat aaagctccgc gcccagatgc agctgcctca tcaggtcggc cagctgcttc
     4441 ggtgtggcgt gcgcgttgct cgccaacggc ccatgggcca ccgatagatc gtcaaacgtg
     4501 aggccgccac cgcagccggc ggtgacgatc gcccgccgcg gccgcacgaa tgtggtgtca
     4561 tggagccaca catcgacgca tgagttgctc ttcgacacat gatgggcgcg cacgtcgtca
     4621 agcctgttga tgacgccctc gttatagaga aatcccaacg ccagttccgt cagatcgcgt
     4681 ggcgtgcaca tgaacgtcgt caactccatg ccgttgaccg acacacaggc cgatacctcg
     4741 tcgatgaccg cgccgtccac caccaacgga tcgccgccat tgaggctgat atagtgcgcc
     4801 ggacgcacgc ccacaggcgt ctcccggtcg tcgaccggcg gtcgttggcc gtcgttcatc
     4861 ttgctcgccc gctgccgcca tccggctcga acagttccgg ccagcggtca atggcgctca
     4921 tcacagccaa cccggctgtc tgccccaggc cgcatagcga cgtctgcgtc atggccaagc
     4981 cgatctcctt caaatccact ctgtcattgg cgagcgggcc gccgtgatgg aggatacgct
     5041 ccgtaatctc catttggcgc tgcgtgccca gcgcgcacgg gtagcatttg ccacaactct
     5101 cgtgggcaaa gaaacgggcc agcatataca tgatctcgcg caggtctacg gcttcatcga
     5161 aaaccatgat aacgccggaa ccgaggggga agccctgggc tttggagtcc tcgtaggtca
     5221 gcggcgtatc gagccgtcgc ggcccgatga acacgcccga cgcgccgccc aacagcacgg
     5281 cctgcacttc cttgccgacg acgccaccgg ccatctggat cagttgtcgc accgtcaggc
     5341 caaacggcac ctcatagacg cccggccgca aaacccgacc gctgacgcag aaccattttg
     5401 tgcccggcga tccggccgtg cccagcttgt gccactgctc gacgcccata ttgaggacgg
     5461 ccaccgtggc ggccagcgtc tcgatgttgt tgacggccgt cggttgttgg aacaggccat
     5521 gggtggtggg gaagggcggt ttgatgcgcg ggaagccacg cttgccttcg atggcttcga
     5581 agagcgccgt ctcttcgcca cagatgtagg cccccgcgcc caggcgcaac tcgatatcga
     5641 aattgaaccc gcggcggccg aggatgttgc gacccagata gcctgcttcg cgcgctttct
     5701 cgatggcgtt ctgtagtcgc ttgtagcagc gcgggtattc gccacgcagg aagatccagc
     5761 cgttctccgc gccaatcgta taggcagcaa ccgtcatgga ttcgatcaga ctgaacgggt
     5821 cttgctccat gagggcgcga tccttgaacg tccccggctc gctctcgtcg gcgttggcca
     5881 cgaggtgctt ttcggacgga agcccgggcg cgttgcgcgt catctcccat ttcagaccca
     5941 cggggaacat cgccccaccg cggccgagca cgtcgctgcc cttcatcatg gccagaatct
     6001 cttccggcgt cattgtcagc gccttgcgca gcgccttata gccgtcgtgc ttcacaaagt
     6061 cagtcagact agttgggtcg atcttcccgg cgcgggccag cttcaccaac gggccgccat
     6121 agattcgcgg ctccggctcg gggtatgtgc cggccaggaa ggcgtcgacg tcagccgggg
     6181 tcagatcacc cgccggtcgg tcgccgttga gcgcgttggg ggcgtgctcg cacatgccca
     6241 ggcaggggac ggtctcgtag ccgatcaggc cgtcctcgct cgtctcgccc ggcttcagtc
     6301 cgagacgctc ctcgatggcc aggcgcacgg ccgggccgcc ggccatgtgg caggcaggat
     6361 cattgcagac ccgaatgacg cgcttgccca tcggctcgtt atagaacatc gagtagaact
     6421 cgaccacgcc gtggatgtcg gacaggggca cgcgcaacgt ttcgccaatg gcttcctgca
     6481 tctcgggcgg cagccagccg tggatcgtct gagcttcgtg taacagggga aggagtgatt
     6541 cgcgaccgcg gccgcgatac cggttaagga tcggctccag cgcggtcata tcaattgtgg
     6601 cgggctgcac catattttgc gcctccttgg agacaagcaa taagccatta aaagttcgtg
     6661 ggcgcaaccg gggaggtcgc gcgtcgtcgg tgcgggatcg gcgacggggt gattataccc
     6721 ggcttgtggg gagggggaat aataaaaggg agaacaatca ggataatccc atataaaacc
     6781 ccgatcctta atggtaacgt gtatgggcgg ggcaaccggc gacagcgctg aagcgcttaa
     6841 ctacaaacgg gttcgcgcca gttgtctcgc ccgtacagat gccatgtgcc gcgtgacggg
     6901 gagggcgaga cagcgcccaa agcagtatga tttcaacgac ttgttggcgc gcgtgggcct
     6961 gcccctactc cagcaaggcc acggcgttgc gatagaacgg gtggcgcgcg gccaacaaat
     7021 gggctgcccg cgcatcctgc tttcggccga ccaggcgata gattaggcca agcgtgaaaa
     7081 agatcgccgg cggggtcagg ccggcggcga tggtcttctc atagaaagag atcgaaaccc
     7141 ccgcgcggcc gtagccctcg ttcaacatcg cctggcgcag gaagatgaac gcctccgggt
     7201 tgggcgagct gctgttggcg tgggcgctct gccgaatctc ggccgccagc tgccattggg
     7261 caaattcggc cgcggcgttg atcagatcac cggactcaat acgcgcgtcc cgatgatcct
     7321 ctcgacggtc ccgcaatgct acgcagcgcc aggcctgatt ggtggccgcc cacactcgcg
     7381 cgtcaccggg ccggtcgctc atcacggtgt agcaaatgtg aagagccatc agacagcggc
     7441 cgtcgccgcg caggatgtcg gccaggtcca ggaagtggcc ggccgcggca tccatgtctc
     7501 cgcgctgccg cgcggccagc gcaagtcgtt cgcgggcagc gataaagtct gcgcgaaggg
     7561 caacggcgta ggcccattgt gcctcggctc gatcaaattg accctggttc cagaacatat
     7621 cgccgctgat tagcaggctg cgggcggcat cagggatcat ccccagatgc tcctgcgccg
     7681 cggctacctt gttgacatac tcggcattgt tcccggccag tcgggcggct tctttatagc
     7741 aggccagcgc ccgaccgcga tcggacaggg ctgtgtgggt atcgcccagg ccgttgtaga
     7801 cgcgcggctc gcgcggcagg ccggtcaggg cggtttggaa gcaggtgagc gcctgctgcc
     7861 atttcccttg gcggatagct tcctcgccga tctcaagcgc cagcgtgtaa cggtgggtgg
     7921 agtcgctcat gatgcgctcg ccaggctgtc gcccgatatc tcgcgggcca gctcttgcag
     7981 atagtccctc atgagtcggg ttcgccgctc cccttcccga cgagcggcgg cggtatgcat
     8041 ggcggccgcc aaccccaata acttgacgta gaaatggtcg atgacgtttt ccttgtcgtt
     8101 gggcgcgcgg tttttcggca gcggttcgtc gtggtcgtag agcggcttgc ccatcatacc
     8161 acccagcagg agcgtgcggg cgatgccgat tgcacccagc gcgtcgagcc ggtcggcgtc
     8221 ctgcacgacc tgggcctcgc gcgtttgggg cggaatgccg gcgctgaagc tgtgggcggc
     8281 gatggcgtgt tcgatggcgg gaatgtactc ggccggataa cccgcctcgc gcaggaaagc
     8341 cccggcgcgc tcggcggcca tttgggaagc acgcgcccga tgcacggagt ccttgggcac
     8401 gacgacgcaa tcatggagcc aggctgcggg cacgacgacg gccggatcag ccccctcggc
     8461 cgcggccagc tgccgagcgt tggcgactac gcgccgaacg tgctcccggt cgtgggccgg
     8521 atcgggcgtt tcctccatgg catgctcgat aaactgctcg aaacgcggtt cccaatcgct
     8581 caacataacg gctattctag cagtggcggc gttaaaaggc gattgggctt tcctatgttt
     8641 ttacatcgat accgcagcat gacggttttc ccttgccgga tgcaatggta tgggtgatgt
     8701 ttggtgatat aatttatcat acttgatata agcaagaggg tatgaagatg aagacgcaag
     8761 aaagacgatc gcgcaaagtg acgatatcgt tgcccgaaga actggtcgtc tataccgacc
     8821 ggcgcgccga tcggaaaggc accaaccgca gccaggtgat cagccaggcg ttggctcact
     8881 tgatggcttt cgaggaggaa caactggcgg ccgaagggta tgcattttac gctgacgagg
     8941 ctgaggagtt cgcctcgtcg tccgatcagg ccatcgccga ggcatgggga cgcgacacag
     9001 aggaggcgca gtcatgatcc ggcgcggcga gatatactgg gtggaatttg accccgtgaa
     9061 agggagcgag caagggggat tgcgtccggc cctcgttatc caaaacgaca ccggcaatcg
     9121 ttatagcccg acgacggttg tcgccgcgat aacgcggaca gtgccgccca agccgtatcc
     9181 gttcacggtc attatagaac ctcatgagag cggtttgcag gcgacgagcg ccatcaactg
     9241 ctcccaggtg gctaccattc aacaaagcgg accgcaaagc cggctgcgag caattcccgg
     9301 cgaaaacggg atacggccga ttggtcgcct aagtgatgcg aaaatgcagg aagtgagcga
     9361 ggcgctccga ttcaactttg gtttgtagta aatggagacg cgttgcggcc gatgcttaaa
     9421 atgtcgtccg cgctccctgc ttattgggcg tgtgcatcag gaaatcgcgg acgaagcgct
     9481 cgttatactc atcgagcagt tgcgacacgc gatcggggtt atccgaagca accagcagcc
     9541 cggcgtggta ctctcgcggc acgcgccaga cgatctccgg ctcgtcgtag gccgacagat
     9601 tcggccactg ctcccgcgcc aggcagatga tcagtccggc ataatcattg cggtgttcag
     9661 gcaattcgta ggcctcgcca cgctcggcgg ccagctcgat ccgcgccgct tcctgccaca
     9721 gggcgatgcc tgtggccgct tccaccatct tgtcgatgtg ggccccgccg acgcgcgcgg
     9781 ccgtctccag gaagtagtat tgcccgtcgt ggtgggcgcg gatgaactcg gtgtgggtga
     9841 cgccttggtt gagaccaaag gcgcgaaaga catcggccgc caggttcgtc gtctcgtgga
     9901 actcttccga gtcgcggggc agcaggcgcg tgttgaagat gccgccgccc tgggtcacgg
     9961 tgagcggcgg cgcgccatag cgactgctga cggtgaagac tacttcgcca tcccacaaca
    10021 gcgagtcgac gtggtagacg tcgccgggca cgaactgctc cagcaagtag aacgactgcc
    10081 cgtcgcccaa ctcgtcgagt gcccgccaaa cgccctcgct gtcctggagc ttgcgaatgc
    10141 caaccgcccc ggcctcgaag cgcggcttca gcacccatgg cggcgagatg gcggccatgt
    10201 aattgcgtaa ctgatcgtaa ttgaaggccg acgtgaaggc cgggacgcgg atgccatgag
    10261 aagcggcctg gacgcgcata ctcagtttgt cgcggaagta gcgggcctgc gtctcgccga
    10321 tgccgggtag ccgcatatgc tcgcgcaacg aagcggccgt ggccacgtcg tagtcatcga
    10381 gagccacgat gcggtcgatc tgattctcgc gggccagata gctgacagcg tgggtgatgc
    10441 cgggctgggt gtgcaggtcg ggcatattgt aatggcgata gatggcctcc cacggccatg
    10501 gctcgctgcc gtacttttcg gtcgtcagga gaatgacccg cgcgccctgc cgggcacact
    10561 cctcgatgaa gggcacgccc ttgaattcgc tggcgagaca gaggatggtg atcggccgtt
    10621 ggtcggtca
//
LOCUS       DAOMQG010000088        10573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2693_length_10573_cov_6.563415, whole genome shotgun sequence.
ACCESSION   DAOMQG010000088 DAOMQG010000000
VERSION     DAOMQG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_10573_cov_6.563415"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PLC06_06835"
     CDS             <1..1891
                     /locus_tag="PLC06_06835"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO90196.1"
                     /translation="LTARFDRLDESARRVLQIAAIIGRHFYRSTLAELVDDPARLDSC
                     LLELQRLELIREVGRVPEPSYLFNHSLTQEAVYNTILLKQRRVLHLRVAEVIEKLRAA
                     NPAAVAAMLAHHFIEGDAPQRGLPYLLTVGASALKLHAVDEALAAYDKALPIARALPD
                     RSAELIAIYSGRGRCLELLSQFAEARAIYEEMEALAVERADPALELEAIIAQGKLLGN
                     VTPLYDPVRGRALMERARLLAEATGNHAAEVRILWNLVNIDRFDVNSLDNAVVNGERG
                     IMLARELGLQEELAYLLNDMSDIYGTSGQVDMARATLVEARALWRTLGNEGMLADTLS
                     NSSIWSSLNGQLPDGLAFAEEANAIATRLGNIWGESYSTGARGLALLLMGLWGRAIAE
                     LRAGIEKAGAANFVGGQIIIGSFLSRIFLDIGDTAGAIDIAQQAIDIAEERLPQFAGI
                     GLGRLAAAQIAQGDIEAAAETLANPLASLEKQQFFVVYDISAGHTRLALTRGLFDETL
                     AQADDAINRLTYVGADVWLPDLYHLRAMALIELERFDEAVVDLTTAIDAARRTGTRGG
                     LWQVLITRTKLARTRGEPDEEWRAAAIAEIDFLAANLYPDELRASFLARPDVARIMQA
                     ADVER"
     gene            1962..2573
                     /locus_tag="PLC06_06840"
     CDS             1962..2573
                     /locus_tag="PLC06_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRO90197.1"
                     /translation="MRELIIDFITSLDGYASADGWPGWWGLEGPEYFAWLGEQPPVTY
                     LMGANTYRLMHGFFAGLTGGEMPAGAAEMGAEEEASVDELTRAPKVVFSATLEEPLAW
                     ANTTLVRGDAVEAVRAMKAEGSGILSTLGSISLARSLLRAGIVDRFRVVMFPVITGAT
                     GAERIYDGYPDVMLEMIDHRTFDGRIQLVEYRPRVLEGPPPAQ"
     gene            2725..3105
                     /locus_tag="PLC06_06845"
     CDS             2725..3105
                     /locus_tag="PLC06_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO90198.1"
                     /translation="MSDDPKLSTIRQIAVTVSDVDLALGFYRDMLGIPFLFSAGPDLA
                     FLDAGGVRLMLSVPQGAGSVGHNSILYFTVSNIAATHAALVARGAPHEEAPALVARMP
                     DHELWISFLRDPDGNLVGLMEEKR"
     gene            3236..3397
                     /locus_tag="PLC06_06850"
     CDS             3236..3397
                     /locus_tag="PLC06_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO90199.1"
                     /translation="MADTYGVIIIGGGINGCSTALQLARRGVKVAVVEKDSIGDGPTG
                     PSAAVMSTA"
     gene            complement(3511..5877)
                     /locus_tag="PLC06_06855"
     CDS             complement(3511..5877)
                     /locus_tag="PLC06_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRO90200.1"
                     /translation="MAQAELQLSLLGGLTITYRNTAVSQFASRKVEGLLVYLACNPRP
                     HPRETLAALLWPDNDQNRALANLSVALTSLRKQLPTAVLAERHTVAFNTEQDYMLDTA
                     AFQRAASQARDEQKRSGKLGHAGAAQLAAAVTLYRGDFLAGFNIRNAPEFEAWVLLEQ
                     ERLRQQFLAALADLVAFHRQRGRLDTAIAYAQQLLAVDPLQEDIHRQLMTLFAQNNQR
                     PAALAQYEQYRRILNEELSVKPAEETVLLYKQIAEGQLSDLSPSSEGENSTALPRADT
                     APHNLLAAGTAFIGRSEELARITTWLQGPNSRLLTIVGPGGMGKSRLAHEAARAQIGQ
                     FADGVWLVSLVKCASRNEMVTAVAEAIGLIFSGQTEPDKQLLSHLQGREMLLVLDNLE
                     HLITPDLLALITDIGQQAPDVRLIVTSRERLRLQAEAVLDLRGLPFPRVVSLSTVVDD
                     EGRDDHLPPAEYSAVQLFVDRAQRVQSSFQLDGQEMAVMQLCRLVGGLPLALELAASW
                     ARVLAVADIVAEIECGLDSLTTTLLDVPERHRSLQAVVDSSWQLLAAEEQQLFRRVSV
                     FRGGFTRAAAEQVAGASLPHLMALVDRSFLRLDGDRRFRRHPLLLQFAQEQLAAEGEE
                     QSRTEAAHARYFATWLQALEPDLQGKDALQTLAAIEADLENCRTAWRWALSTANWERL
                     LQLVGGLGRFFEYRSRYLEGTALFSDGLQVLEQAAAVPSALIAGMQTYLGTFLYHNGR
                     RAEAEAALREACRLAQQHDLVQTRILALSYLGEVMGEWGEREAAPSCN"
     gene            6071..6286
                     /locus_tag="PLC06_06860"
     CDS             6071..6286
                     /locus_tag="PLC06_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90201.1"
                     /translation="MPTREYKAYLLRLWRESHEAAWRAMLENPNSNERIAFATLGELV
                     AYLEEKTGEQIRPLPIEQSSASEMSRL"
     gene            6312..7214
                     /locus_tag="PLC06_06865"
     CDS             6312..7214
                     /locus_tag="PLC06_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRO90202.1"
                     /translation="MKILKWVGIVIGVLIALVVVVGLIGYIRGGSMLTRKFDYTPDNI
                     TVPTDAEAVANGEYLVKHFMLCADCHGENLAGKEFINDPSFATIYTPNLTSGQGGIGA
                     TYTDADWIRALRHGIRPNGEVLMIMPSNEYTHADAGELGDMIAYLKTLPAVDNPIPAR
                     KLTTVPRILMGLGVIPAAELLPAMSIDHNAPPTTAPARGATAEYGAYRVLTCKGCHSP
                     NLAGAPADAQTGLPAAPNLTPGGEIAGWTEEEFLTTLATGVTPSGRELSPAMPWPAIG
                     TADKEDLQAIWAYLQTLPSTPTNN"
     gene            7404..8579
                     /locus_tag="PLC06_06870"
     CDS             7404..8579
                     /locus_tag="PLC06_06870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRO90203.1"
                     /translation="MAETYDVIIIGGGINGCSTALELARRGVKVAVVEKDNIGDGPTG
                     RSSAVIRMHYSNELTARMAYYSLGVFSDFEERVGGECGFRRSGFLALASADNREGLVA
                     NVAMQRAIGIRTEVITPEAVREIMPGLETADTVAAAWEEDSGYADPYLTVTSYAAAAK
                     RAGARLLINTAVTGITRHGDRVTGVTTTAGELSAPVVVNCAGPWGARVARMAGLDIPV
                     NACRVQISLFRRPSGEEAAHPVVIDFTNATYFRSETGNLTLVGVVDPAEANAVVNPDK
                     YDESVDTDFILDTGERLIRRYPAMERSDSAGGYAAMYDVTPDWHVIADEPLPGSGFFL
                     CCGSSGHGFKLGPATGLMVADRVMGVANPLFDTHLFRLGRFADDEPVRGRYEYSIAG"
     gene            8816..10480
                     /locus_tag="PLC06_06875"
     CDS             8816..10480
                     /locus_tag="PLC06_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO90204.1"
                     /translation="MIKPVILTLDDEPQVLNAVARDLRAHFRNDYRIVSASSGKDALD
                     ALRQLKQRNTPVALFLADQRMPQMTGVEFLEAAQVFYPDARKVLLTAYADTEAAIASI
                     NKVGLDHYLMKPWDPPEINLYPILDDLLSDWWATTPLPFEGIRVAGTLWSPASHNLKD
                     FLARNRIPYQWLDVEKDTEAHALVEKQFTGGAARFPVVFFPDGTTLIEPSLTDVAAKA
                     GLQTVAGAPFYDMIIIGGGPAGLGAAVYGASEGLSTVMIEREATGGQAGTSSRIENYL
                     GFPRGLSGSDLATRAVAQARRLGAEILTTREVVGVRVEDPYRYVTLNDGTELGCKALV
                     VATGVTTRRLEAPGVAELTGAGIYYGAALTEAASYRGEHMVVVGGANSAGQGAMFFSR
                     YASKVTILVRSSSLSKSMSQYLIDQIAATDNIEVLPYHQVVEAHGQNRLEAISVLNAE
                     TNEVTRMETPALFLFIGAVPHSDCVAGVVDRNSAGFILAGPDLINDGKRPKGWKAQRD
                     PFLLETSVPGIFVAGDVRQGATRRVAAAVGEGANVVSQVHQYLRTV"
BASE COUNT         1982 a         3455 c         3232 g         1904 t
ORIGIN      
        1 gctaacggcg cgcttcgacc gcctcgacga atcggcccgg cgggtgctgc aaattgcggc
       61 gatcatcggc cgccacttct accgctccac gctggcggag ttggtggacg atcccgcccg
      121 cctcgacagc tgcctgctgg agctgcaacg gctggaactc atccgtgagg tcggccgcgt
      181 gcccgaaccg agctatctgt tcaaccacag cctgacccag gaagcggtct ataacaccat
      241 cctgctgaag cagcgccggg tgctgcactt gcgcgtggcc gaagtgattg aaaagctgcg
      301 cgccgccaac ccggcagccg tcgccgccat gctggcccac cacttcattg agggggacgc
      361 cccccagcga ggtctgccct atttgctgac ggtcggcgca tccgctctga agctccatgc
      421 cgtcgacgaa gccctcgctg cctatgacaa ggccctgccc atcgcccgcg cgctgcccga
      481 ccgttcggcc gagctgatcg caatctactc cggtcgcggc cgttgtttag agctgctttc
      541 ccaatttgcc gaggccaggg cgatctatga ggagatggag gcgctggccg tggagcgggc
      601 cgacccggcg ctggagctgg aggcgatcat tgcccagggc aagctcctgg gcaatgtgac
      661 gcctctctat gaccctgtgc gcggccgcgc cttgatggag cgcgcccggt tgctggccga
      721 ggccaccggt aaccacgccg ccgaggtgcg catcctctgg aatctggtca acatcgaccg
      781 ctttgacgtc aattcactgg acaacgccgt tgtcaacggc gagcgtggta tcatgctggc
      841 ccgtgaactc ggcctgcagg aggagttggc ctacctgctc aacgacatga gcgacatcta
      901 cggcacgtcg ggtcaggtcg atatggcgcg cgccaccctg gtcgaagctc gcgctctctg
      961 gcgaacgctg ggcaacgagg gcatgctggc cgacaccctg tccaactcct cgatttggtc
     1021 atcgctcaac ggccaactgc ccgatgggct ggcttttgcc gaagaagcca acgccatcgc
     1081 cacccgtctg ggcaacattt ggggcgagtc ctacagcaca ggggcgcggg gtcttgcatt
     1141 gctcctgatg gggttgtggg gtcgggcaat cgccgaactg cgagcgggaa tagagaaagc
     1201 cggcgcggcc aacttcgtcg gcggacagat cattatcgga tcgttcctgt cgcgcatctt
     1261 ccttgatatc ggcgacaccg ccggcgcgat tgacattgcc caacaggcaa tcgatatcgc
     1321 cgaggagcgg ctgccccagt ttgccggcat cggcctcggc cgcctggctg ccgcccagat
     1381 cgcccagggc gatattgaag ccgcggccga aacgctggcc aatccgctgg cctcgctgga
     1441 gaagcagcaa ttcttcgtcg tctacgacat cagcgccggc catacccgtc tggcattgac
     1501 tcgcgggctg ttcgacgaga cgctggcgca ggctgatgac gcgatcaacc ggctgaccta
     1561 cgtcggggcc gatgtttggc tgcccgatct ctatcatctg cgggccatgg cgctgattga
     1621 attggagcgg ttcgatgaag ccgtcgtcga tctgaccacg gccatcgacg cggcgcggcg
     1681 gacagggacc cgcggtggtc tctggcaagt cctgatcact cgcaccaagc tggcccggac
     1741 tcgcggcgag ccggatgagg aatggcgcgc cgcggccatt gccgagatcg acttcctggc
     1801 cgccaacctc taccccgacg agctgcgcgc ctcgtttctg gcgcggccgg atgtggcgcg
     1861 gattatgcag gcggccgacg tggaacgtta acaggcttgt tcaataagga cacaacgtcc
     1921 gaaggacaca acgtccaatc catcacctga ggagaatcac gatgcgagag cttataatcg
     1981 atttcatcac ctctctggac ggctacgcgt cggccgatgg ctggcccggc tggtggggcc
     2041 tcgaagggcc ggagtacttc gcctggctcg gcgaacaacc gccggtgacc tacctgatgg
     2101 gcgcgaacac ctaccgactg atgcacggtt tcttcgccgg cctcaccggg ggagagatgc
     2161 cggcgggagc ggccgagatg ggagccgagg aggaggccag cgtcgacgaa ctcacgcgag
     2221 cgccgaaggt ggtgttctcg gccacgctcg aggagcctct ggcctgggcc aacacgaccc
     2281 tcgtgcgcgg cgacgccgtg gaggcggtgc gggcgatgaa agcggaaggc tccggcatcc
     2341 tcagcacact cggcagcatc agcctggccc gttcgctgct gcgcgccggg atcgtggatc
     2401 gctttcgggt cgtgatgttc ccggtgatca ccggcgctac cggcgcggag cgcatctacg
     2461 acggctatcc cgacgtcatg ctcgagatga tcgaccatcg cacctttgac ggccgcatcc
     2521 agctcgtcga gtatcggccg cgcgtgctgg aggggccgcc cccggcgcaa tagctccagg
     2581 attgagcagc aggatggctc ctgtttatgg ttctttttgc ctgtctccgg cttcaggttt
     2641 atactgctct cgcgcagcat cgcaccctgt ccgcttgcga ctcacaccca tcgtcagatg
     2701 ccacaataga cttggggaca tatcatgagc gacgatccaa aactctccac aatccgtcag
     2761 atcgcggtga ccgtcagtga cgttgacctc gccctggggt tctaccgcga catgctgggt
     2821 atcccctttc tcttcagcgc cggccccgac cttgcttttc tcgatgccgg cggcgttcgc
     2881 ctgatgctca gcgttccgca aggagccggg tctgtcggcc ataattccat tctctatttc
     2941 acggtttcga acatcgccgc aacccacgcc gcactggttg cgcgcggcgc cccacatgaa
     3001 gaagcacctg ctctcgtcgc aaggatgccg gaccatgaac tatggataag cttcctgaga
     3061 gacccggacg gcaacctcgt cggtctgatg gaggaaaagc ggtaactccg cacactcgag
     3121 ccgccccgta attcacactg aatcgggccg ttttgccgag cgaatttctt accggtcatc
     3181 cccctacatg ctatactccc cccacgcggg aaccaattca aagggagttt catcaatggc
     3241 tgatacatat ggcgtaatca tcatcggcgg cggcatcaac ggctgttcca ccgccttgca
     3301 gctggcccgg cgcggggtca aggtcgcggt cgtggagaag gacagcatcg gcgacggccc
     3361 caccggcccc tcggcggccg tcatgtcgac ggcatagccg gcttgcgtct gcccataatc
     3421 gccgttgttc ttcagcaaga tgccgcgccg ccacaacagc agcagcttct cctcatcgtc
     3481 gacgcgcggc cggcagatct ggagcgcttc ttaattacag gagggggcag cctcgcgttc
     3541 gccccactcg cccatgactt cgcccagata gctcagggcc aggatgcggg tttgaacaag
     3601 gtcgtgctgt tgggccagcc ggcaggcctc acgcagggcg gcttcggctt cggcccggcg
     3661 gccgttgtgg tacagaaaag tccccaggta tgtctgcatc ccggcgatga gcgcggaggg
     3721 aacggccgcc gcctgctcca gcacctgcaa cccgtccgaa aatagcgccg tcccttccag
     3781 atagcggctg cgatactcga agaagcggcc gagaccgccg accaactgga gcagccgctc
     3841 ccaattcgcg gtcgacaaag cccagcgcca ggccgtccgg caattctcca gatccgcttc
     3901 gatggcggcc agggtctgga gcgcatcctt gccctgcaag tccggctcca gcgcctgcag
     3961 ccaggtcgca aaatagcggg catgggccgc ttccgtgcgg gattgctctt ccccttcggc
     4021 ggccagctgc tcctgggcga attgcagcag cagcgggtgg cggcgaaaac ggcggtcgcc
     4081 atccagccgc aaaaaggagc gatccaccaa cgccatcaag tgcggcaggc tcgccccggc
     4141 cacctgttcc gcggccgcgc gggtgaaacc gccacgaaag acggacaccc gccgaaacag
     4201 ctgctgctcc tcggccgcca gcaactgcca cgagctgtcg acaaccgcct gcaggctgcg
     4261 atggcgttcg ggcacatcga gcaaggttgt tgtcaggctg tccagcccac actcaatctc
     4321 ggccacaata tcggccaccg ccagcacgcg cgcccaactc gccgccagtt ccaaagccaa
     4381 cggcaggccg ccaacgagcc ggcacaactg catgacggcc atctcttgtc catccagctg
     4441 gaacgaggac tggacacgtt gggcgcggtc gacaaagagc tgcacggccg aatactccgc
     4501 cggcggcaaa tgatcatcgc ggccctcatc atcgacgacg gtcgacaggc tgacgaccct
     4561 ggggaacggc agcccccgca aatccagcac cgcctccgct tgcaggcgca gtcgttcgcg
     4621 gctggtgacg atgaggcgca cgtcgggggc ttgttggccg atatcggtga tgagggccaa
     4681 cagatcgggg gtgatcaggt gttccagatt gtccagcacc agcagcattt cgcggccttg
     4741 cagatggctg agcagctgct tgtccggttc agtctgaccg gagaagatga ggccgatggc
     4801 ttcggcgacg gccgtcacca tctcattccg cgacgcgcat ttcaccagcg acacgagcca
     4861 cacgccgtcg gcgaactgcc caatctgtgc ccgtgccgcc tcatgggcca gccgcgattt
     4921 gcccattccc cccggcccaa caatggtgag cagacggctg ttcggcccct gcagccaggt
     4981 tgtgatccgc gccagctcct cggagcggcc gatgaaagcg gtgccggcag cgagcaggtt
     5041 gtgcggggcg gtgtcggcgc gggggagagc agttgaattt tccccctcac tcgagggcga
     5101 cagatcgctc agctgcccct ccgcgatctg cttgtagagc aaaaccgtct cctcggccgg
     5161 cttcacgctc aattcctcgt tcaggatacg gcgatattgc tcatattggg ccagggcagc
     5221 cggccgttga ttgttttggg caaagagcgt catcaattgc cggtgaatgt cttcctgcaa
     5281 tgggtcaacg gccagcagtt gttgggcata ggcaatggcc gtatccaatc ggccgcgctg
     5341 ccggtggaag gcaaccagat cggccagagc agcgaggaac tgttggcgca agcgctcctg
     5401 ttccagcagc acccacgctt caaattcagg cgcgttgcgg atgttgaagc cggccaggaa
     5461 atcgccgcga tagagggtga cggccgccgc caattgggcc gcgccggcat gccccagttt
     5521 gccggacctc ttttgctcgt cccttgcctg gctggcggct cgctggaatg cggccgtatc
     5581 cagcatgtag tcctgttcgg tattgaatgc gaccgtatgt cgctcggcaa gcacggccgt
     5641 cggtaactgc ttgcgcaaac tggtgagggc gacgctgaga ttggccaacg cgcggttctg
     5701 atcgttgtcc ggccaaagaa gcgcggccaa cgtttcacgg gggtgcggtc gcgggttgca
     5761 ggccagataa acgagcaagc cctcgacctt gcgcgaggca aattgggaaa cggccgtgtt
     5821 ccgataggta atcgtcaacc cgcccaacaa cgaaagctgc aattcagctt gtgccatgtc
     5881 tgcaatcgtg ttccagcttc tctctcaaaa agacagaaaa taaagagttc ctaaagagcg
     5941 tcacgctaga ataaccatga gtaaaatgtc ttgccatctc cacgggcggt atgattatag
     6001 caatgctgga accgggagga aatggcattg aacaattgac gtaacggaaa caaattcagg
     6061 aaccaacccc atgcccacgc gcgaatacaa agcctatctg cttagactgt ggcgcgagag
     6121 ccacgaagca gcctggcggg ccatgctgga aaatccaaac agtaatgagc ggatcgcgtt
     6181 tgccacattg ggggagctgg ttgcctattt ggaggagaaa acgggggaac agattcgacc
     6241 gctgcctata gaacaatcat cggcatcaga gatgagtcga ttgtaaatga ccacttttta
     6301 tggaggctcc tgtgaaaatt ttgaaatggg ttggaattgt tatcggtgtg ttgatcgcct
     6361 tggtcgtggt cgttggcctg attggataca tccggggcgg gtcaatgctg accaggaaat
     6421 ttgactatac gccggataat atcaccgtgc cgaccgacgc cgaggccgtg gccaatggtg
     6481 aatacctggt gaagcacttc atgctttgtg ccgactgtca tggcgaaaac ctggccggca
     6541 aggagtttat caatgatccg agctttgcca ccatctacac gcctaacctg accagtggac
     6601 agggcggcat cggcgcgact tacaccgacg ccgactggat tcgtgccttg cgccacggaa
     6661 ttcgcccgaa cggtgaagtg ctgatgatca tgccatcgaa tgaatacacg cacgcggatg
     6721 caggtgagtt aggcgatatg atcgcttatc tcaagacgct gccggccgtt gacaatccga
     6781 ttccggcccg caagctcacc accgttcccc gcatcctgat ggggttgggc gtcatccccg
     6841 cggccgagct tcttccggcg atgagcatcg atcacaacgc cccgccaacc acggccccgg
     6901 cgcgcggcgc caccgccgaa tatggcgcgt atcgggtact gacctgtaag ggctgccaca
     6961 gccctaatct ggccggcgcg ccggctgatg cccagacagg tttgcctgcc gcccccaacc
     7021 tgaccccggg cggcgagata gccggttgga cggaagaaga gttcctgacc acgctggcca
     7081 ccggcgtgac gccaagcggc agggagctaa gccccgccat gccctggccc gccatcggca
     7141 cggccgacaa ggaagatttg caggcgatct gggcctatct gcagacactt ccgtcgactc
     7201 cgacaaacaa ttagcatagc gggttttctt cgcgatttcc aacgggcatg gagttttcca
     7261 tgcccgttgt gtttttacgc ctgggcagct ccccgataat tcattttgaa gcagaccggt
     7321 ttatggatcg aaattcttac cggccactcc ccatcatgct atactccccc acgcgggaac
     7381 catttcaaac gggagtttca tcaatggcag aaacatatga cgtaatcatc atcggcgggg
     7441 gcatcaacgg ctgctccacc gctctggaac tggcgcggcg cggcgtcaag gtcgccgtcg
     7501 tcgagaaaga caacatcggc gacggaccga ccggtcgctc ttcggccgtc atccgcatgc
     7561 actactccaa cgagctgacc gcccgcatgg cctactacag cctgggcgtc ttcagcgact
     7621 ttgaggagcg ggtcggcggc gagtgcggct tccggcgcag cggctttctg gcgctggctt
     7681 cggccgacaa ccgcgagggg ctggtggcca acgtcgccat gcagcgcgcc atcggcatcc
     7741 gcaccgaggt gatcacgccc gaagcggtgc gcgagatcat gcccggcctg gagacggccg
     7801 atacagtcgc cgccgcctgg gaagaggaca gcggctacgc cgacccctat ctgaccgtca
     7861 cttcctacgc cgccgcagcc aagcgcgccg gggcacgtct gctgatcaac accgccgtca
     7921 ccggcatcac ccgtcacggc gaccgcgtca ccggcgtgac gacgacggcc ggggagctat
     7981 ccgcgccggt cgtggtcaat tgcgccgggc cgtggggggc gcgcgtggcg cgcatggcgg
     8041 ggctggatat tcccgtcaac gcttgccggg tacagatttc gctcttcagg cggccgagcg
     8101 gcgaagaagc ggcccacccc gtcgtcatcg acttcaccaa cgccacctac ttccgcagcg
     8161 agacgggcaa cctgaccctc gtgggggtcg tcgatccggc cgaggccaac gccgtcgtca
     8221 accccgacaa gtatgacgag agcgtcgaca ccgacttcat cctcgacacc ggcgagcggc
     8281 tcattcgccg ctacccggcc atggagcgca gcgacagcgc cggcggctat gcggcgatgt
     8341 acgacgtcac ccccgactgg cacgtcatcg ccgacgaacc gttgcccggc agcggcttct
     8401 tcctgtgctg cggctccagc ggccacggct tcaagctcgg cccggccacc ggcctgatgg
     8461 tcgccgaccg ggtgatgggc gtcgccaatc cgctgttcga cacccacctg ttccgtctcg
     8521 gccgcttcgc cgacgatgag ccggtgcgcg ggcgatatga gtacagtatc gcgggataga
     8581 atctgtcgcc cgcgatgctg tactcataca gcatcgccgg ttaataacaa ggtttggaaa
     8641 ccgggtttct tccgggaaac ccggtttcca ggaactccct ccgacttgtg cattccccct
     8701 ttcctcgcta cacttaattt ccggaattac ccacctcgca agtgacccta tagcgccatc
     8761 gacgaagcca acttcgttcg gcgtggctgc ttgcatcaac cgcggaggaa gttaaatgat
     8821 caagcctgtc atcctgaccc tggacgatga accgcaggtc ttgaacgctg tcgctcggga
     8881 tctgcgcgcc cattttcgca acgattatcg cattgtcagc gcaagctccg gcaaggatgc
     8941 cctggacgcg ctgcgacaat tgaaacaacg caacacgccg gtggccctct ttttggccga
     9001 ccagcgcatg ccgcaaatga ccggcgtgga gttcctggaa gcagcccagg tattctaccc
     9061 cgacgcccgc aaggttctgc tgaccgccta cgccgacacc gaggccgcca tcgccagcat
     9121 caacaaggtc ggcctcgacc actatctgat gaagccctgg gatccgccgg agatcaacct
     9181 ctatccgatc ctcgacgacc tgctgagcga ctggtgggcc acgacccccc tgccgttcga
     9241 ggggatccgc gtggccggca cgctctggtc gcccgcttcc cacaacctga aggatttcct
     9301 ggcccgcaat cgcatcccgt atcagtggct cgacgtggag aaggacaccg aggcccacgc
     9361 gctggtggaa aaacagttca ccggcggcgc ggcccgcttt cccgtggtct tcttccccga
     9421 cgggacgacg ctcatcgagc caagcctgac cgacgtggcc gcgaaggccg ggctgcagac
     9481 ggtggccggc gctcctttct atgacatgat catcatcggc ggcggcccgg ccgggctggg
     9541 cgcggccgtc tatggcgcgt cggaagggct atcgacggtc atgatcgaac gtgaggcgac
     9601 cggcggccag gccggcacca gctcgcgcat cgagaactat ctcggcttcc cgcgcggcct
     9661 ctccggctcc gacctggcga cgcgcgccgt agcccaggcc cgccgcctgg gcgcggagat
     9721 attgacgacc cgtgaagtcg tcggcgttcg cgtggaagac ccctatcgct atgtgacgct
     9781 caacgacggc acggaactgg gctgcaaggc gctggtcgtg gccaccggcg tcaccacacg
     9841 gcggctggaa gcgcccggcg tggccgaact gaccggcgcg ggcatctact acggcgcggc
     9901 gctcaccgaa gccgcctcct atcgcggcga gcatatggtg gtcgtcggcg gggccaattc
     9961 cgccgggcaa ggggcgatgt tcttctcgcg ctatgcgtcc aaagtgacca tcctggtgcg
    10021 ctcatcctcg ctgtccaaga gcatgtcgca atacctcatc gaccagatcg cggccacgga
    10081 caacatcgag gtgctgccct accatcaggt cgtcgaggca cacggccaaa atcgactgga
    10141 ggcgatctcc gtcctcaatg cggagacgaa cgaagtgacg cgcatggaga cgccggccct
    10201 gttcctgttc atcggcgcgg tgccccacag cgattgcgtc gccggggtcg tggatcgcaa
    10261 cagcgcgggc ttcatcctcg ccgggccgga cctgatcaac gacggcaagc ggccgaaagg
    10321 ctggaaggcg cagcgcgatc cctttctgct ggagacgagc gtgcccggca tcttcgtcgc
    10381 cggtgacgtg cgccagggcg cgacccgccg ggtggccgcg gccgtcggcg agggggccaa
    10441 cgtcgtcagc caggtgcatc agtatttgcg aactgtctag gcccacatgc atccggggcg
    10501 ggagtgtcac acgctcccgc ccccggatta cccccatccc tacgccaacc tgacgcgctc
    10561 cccatgccac acc
//
LOCUS       DAOMQG010000089        10558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2698_length_10558_cov_10.218699, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000089 DAOMQG010000000
VERSION     DAOMQG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2698_length_10558_cov_10.218699"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1544)
                     /locus_tag="PLC06_06880"
     CDS             complement(180..1544)
                     /locus_tag="PLC06_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO90205.1"
                     /translation="MTTSSNDKLRREGDRLDADGGIGKPGCPHCDGLGYVVAAVGPDE
                     PGFGRAIPCVCRAAEQEKARLDNLMRISQIGALAHCTFESFIPEGHGLTAARQRNLRM
                     AYERAAAYANAPSGWLLLKGGYGCGKTHLAAAIANRQLATGRPVLFVNTPDLLDHLRA
                     TFNPESTVSYDERFDQVRNSSLLILDDLGTQSNSEWAQEKLYQILNYRYNAKLPTVIT
                     TNLDLEAIEIRIRSRMVDPDLSQIIHIAAPDFRRAGVGSDQSDLSTLNLHGDKTFESF
                     DLRENELPRPQADNLHRALQLAVAFADRPIDWLVFNSVAYGNGKTHLAAAIANYVADT
                     GEPVLFVVVPDLLDYLRASFSPSSQARLDKRFDEVKNAPLLVLDDLGTESATAWAREK
                     LYQLFNYRYSARLPTVITSAVAIEQLDPRLASRMLDGQRCAFFVLEAPSYRGKGARGK
                     RRRN"
     gene            complement(1522..>1839)
                     /locus_tag="PLC06_06885"
     CDS             complement(1522..>1839)
                     /locus_tag="PLC06_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRO90206.1"
                     /translation="SPDDEQPITLMVERPNIFVLYEQNIGALTPLIADELREAERTYP
                     PQWIEEAIKLAVTNNARRWRYIMAILERWRQEGRQDALGQRDSQRNLQRQIPSEYDDI
                     IQR"
     assembly_gap    1840..1939
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1940..2405)
                     /locus_tag="PLC06_06890"
     CDS             complement(<1940..2405)
                     /locus_tag="PLC06_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90207.1"
                     /translation="MAGSTSMQGFPGFPDGKQRLTPVPNLFFSDLLPAIDNLAELKVT
                     LYAFWALGQREGAVRYLRLPDFMNDPILLKAMGGPNIQSGVDTLLDGIERAVARGTFL
                     QVTVEGADGQMALYFINTERGRAAVEGISRGEWRPSPDDEQPITLMVERPNIF"
     gene            complement(2497..5127)
                     /locus_tag="PLC06_06895"
     CDS             complement(2497..5127)
                     /locus_tag="PLC06_06895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRO90208.1"
                     /translation="MDTIEERLPPHSVEAEEAVLGSLLIDPDAIFEVSSFLRPDAFYR
                     AQNRWIYEAILALNERRVPLDVVTLIDELRRREQLDEIGGEPTVISLLNAVPTSINVE
                     AYGKMVEAASTRRNLILAAGAIAKLAYNEAEDIDVVLDRSEHALFSISEKRTNRDLKP
                     LKEIAGDYLERIELLRDRGDEFIGIPTGFTDLDRMLSGLNKSDLVIIAARPSMGKTAL
                     QNAIALNAARRYDKRVAMFNLEMSGEQLVQRMIAAETRIDSQRLRRGDLADHEWAIFL
                     EALGRLSESRIFIDDTPSITPMQLRTKCRRLYAEHGLDLIMIDYLQLMSAERSTNNRV
                     QEVSEISRELKGLARELDIPVVAAAQLSRAVEQRQNKRPVLSDLRDSGCLTGDTLVTL
                     ADSGERIPMRDLVGRSDFAVWAMNPTTYRLERAVVSKAFSTGTKPTYRLTTRLGRTIR
                     ATGNHLFMTIKGWERLDELAAGVYIALPRAMSGTDEQTMGDAELALLGHLIGDGCTLP
                     RHAIQYTTKESDLAEMVSDLATTVFGSEVRPRIQQERTWYQVYLSSTRHHTHGTRSAV
                     SEWLDAMRAWGLRSYEKRVPGRVFQQPSHAIALFLRHLWCTDGCMRMTWGKSPRPAIY
                     YATSSEQLARDVQSLLLRLGINGMLKRVSQGDKGRAQYHIVVGGKGDVLRFAHSIGAV
                     NPAREERLDEIIAYIEEREENTNRDVIPQPVWDAHVRPAMQAAGVTQRALYAGIGTPY
                     GGTTIMNQNLSRERAARVAQAAESAELQALAESDVYWDLIVSIEPDAIEEVFDLTVPG
                     LHNFIANDIVVHNSIEQDADVVMFIYRDDYYNPDTSERPNIAEINVAKHRNGPTGSID
                     LFWHGKLAAFRNLHKQEIEL"
     gene            complement(5218..5751)
                     /locus_tag="PLC06_06900"
     CDS             complement(5218..5751)
                     /locus_tag="PLC06_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRO90209.1"
                     /translation="MIEVMIESIRISLVSQHRIVLLREVDSERQLPIWIGPCEADAIT
                     IELQDVKVARPLTQDLLKNVITELGGTLSHVLIKELNESVFHARLFINMEGEREPREI
                     DCRPSDAIALAVRAKVPIFISEEVMDVAGVEPEPDMQEGSDDAEGADDIGPFDAFSDF
                     LDTLDFDDFAGGDDERP"
     gene            complement(5862..6185)
                     /locus_tag="PLC06_06905"
     CDS             complement(5862..6185)
                     /locus_tag="PLC06_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRO90210.1"
                     /translation="MIHQLRIYEIFEHNKAAFHDRFRDHAWRIMQSYSFHILGIWEAQ
                     TDGRTEFYYLLSWPDEATMRAGWAAFLADEEWKAIKRETAARHGDLVGTIEERVLFPT
                     SYSPI"
     gene            6312..7223
                     /locus_tag="PLC06_06910"
     CDS             6312..7223
                     /locus_tag="PLC06_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRO90211.1"
                     /translation="MKPIHYGALLILGAVWGASFLFIGVAVPEFGPLRLMFIRVLLAG
                     LILLGVARLTLRQTSLRDTLRLRENWRKYLIVGLLNSALPFTLIAFSELRLSVSLAAI
                     LNSTTPLFTALVAAAWGSEQLTGRKIVGVLLGVVGVGVLVGGAPLTLDRGILMAVLAS
                     LLAALFYGMGAVYAARHISSLPAAYASTTQLLAAAVWLAVPAAVAPAIRPSPMAVGAL
                     AALILVSTTFAYVLYFFLLKNIGPTRTASVTFLVPVFGSTWAIIFLHEPFSLSMLLGL
                     AIILLSVGLVTGARFGRGAAAVPPVDA"
     gene            complement(7232..9049)
                     /locus_tag="PLC06_06915"
     CDS             complement(7232..9049)
                     /locus_tag="PLC06_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90212.1"
                     /translation="MSCTFGRLIPSGAMQKLALVILALFATGLVACNRPTGAAPVVIG
                     SESTATEPSVAVDTAIAPVAPTDTLAPALPPTDLPIPTAEPSPTAVADPTEAPDLAIN
                     YTDLILHPVPKIYAGDKVTFQLLPHVPDNLHVNEVMAAILVDGVQVAGGPLDRRNWNG
                     QAEGVYEWAWDTTGLSGDYRIEVVLDPGDAIQAGDEDPSNNTISIIATVHVPTGQAAR
                     DLTATWETMETNCCVLHVVSDTAAARDLGSLAPLLEQAVAQAANRLGVTPSQKLHVYF
                     IDRIVGQGGFAGTDMVVSYVDRRYAGGGFYELLVHEAAHILDQQFAPQRISFLAEGLA
                     VWVTGGHYKVEDLKQRSAALLALGQYVPLASLISDFYPVQHEIGYLEAAGFVSYLIER
                     GGWNTFRDFYSEVSADDGPTLADAVDVNLQRYYGATLAQIEAEWMAALQSVPVDPAAV
                     DDLRTTIRYYNVARHYQEIYDPTAHYLSAWLPHPTQVREFGNPADLTRRPEEEINIML
                     EVMLTAADTALRGGDFSRANVILDSVNNILNNNGAIIDPMATSYLNIVRAAAEYGYEL
                     HTVDLQGNVAYAMATQAPYIKLVRLVMEQRGTDWVILSH"
     gene            9366..>10558
                     /gene="icd"
                     /locus_tag="PLC06_06920"
     CDS             9366..>10558
                     /gene="icd"
                     /locus_tag="PLC06_06920"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HRO90213.1"
                     /translation="MTFKNIQLPAGEKITYENGRLHVPDHPIVAFIEGDGIGVDITPA
                     SMMVWDAAVEKAYGGRRKIAWMEIYSGEKAAEKYDGNYMPEETFDAMREYIVGIKGPL
                     TTPIGGGFRSLNVTLRQVLDLYACVRPVRYIPGTPSPVKHPEKMNIVIYRENTEDVYS
                     GVEWEANTEEAKAIIEFMNTKLGKNVREGSAIGIKPVSEFGSKRLVRQAIQYAIEHNR
                     PSVTFVHKGNIMKFTEGAFKNWGYEVAAQEFGDYTVTEDDLWSKYDGKAPEGKIVIND
                     RIADNMLQQMLTRTDEYSVLATLNLNGDYISDAAAAQVGGLGMAPGGNIGDGVALFEA
                     THGTAPKYAGKDMVNPGSLILSGSMMLEYMGWQEAADLVEQAMAKTIQDKVVTYDLHR
                     QIEGAT"
BASE COUNT         1904 a         3154 c         3325 g         2075 t
ORIGIN      
        1 tacaggagat attatagcca ttgtggccgg caatggacac aagcgggcta ttctgtcggg
       61 gaggtggtgt tcggccgctt cccggaggga atttgcacct attattatga aacatacccc
      121 aatgaaaagc gcgccacccc ggcgcgcctc ttcaattcct aattcctaat tcgtaattcc
      181 taattccttc gcctcttccc tctcgcgccc ttgccgcgat agctcggcgc ttccagcacg
      241 aagaaggcac agcgctgacc gtcgagcatg cgcgaagcca gccgtgggtc gagttgctca
      301 atggccacag cgctggtgat aacagtaggc agccgggcgc tgtagcgata gttgaacagc
      361 tggtacagct tctcccgcgc ccaggcggtg gcgctctccg tgcccagatc gtcgagcacc
      421 agcagcggcg cgttcttcac ctcgtcgaag cgcttgtcga gccgagcctg gctgctggga
      481 ctgaacgacg cccgcagata gtcgagcaga tcgggcacga cgacgaacag caccggctcg
      541 cccgtgtcgg ccacatagtt ggcgatggcc gccgccagat gggtcttgcc gttgccatag
      601 gccacactgt tgaacaccag ccagtcgatt gggcggtcgg cgaaggccac ggccagctgc
      661 agcgcccggt ggaggttgtc ggcctgcggc cgcggcagct cgttctcgcg caggtcgaac
      721 gattcgaacg tcttgtcgcc gtggaggttc agcgtcgaca ggtcggactg atcgctgccc
      781 acccccgccc ggcgaaaatc gggcgcggcg atgtggatga tctgcgacag gtcggggtcg
      841 accatgcgcg agcggatgcg aatctcaatc gcttccagat cgagattggt ggtgatgacc
      901 gtcggcagct tggcgttgta gcgatagttg aggatctgat acagcttttc ctgcgcccac
      961 tcgctgttgc tctgcgtgcc caggtcgtcg aggatcagga gcgaactgtt gcgcacctgg
     1021 tcgaagcgct cgtcgtagct gactgtcgat tcggggttga atgtggcccg cagatggtcg
     1081 agcaggtcgg gggtgttgac gaacagcacc ggtcggcccg tggccaattg acgattggcg
     1141 atggccgccg ccaaatgggt cttgccgcag ccgtagccgc ccttcagcag gagccagccg
     1201 gacggcgcgt tggcataggc ggccgcgcgc tcataggcca tgcgcaggtt gcgttgacgc
     1261 gcggcggtca gcccgtggcc ttcggggatg aagctctcga aggtgcaatg ggccagcgcc
     1321 ccgatctggc tgatgcgcat gaggttgtcg aggcgggcct tctcctgctc ggccgcgcga
     1381 cacacgcagg ggatggcgcg gccgaagccg ggttcgtccg gccccacggc ggccacgaca
     1441 tagcccaacc cgtcgcaatg ggggcagccg ggcttgccga tgccgccgtc ggcgtcgaga
     1501 cgatcgccct cgcggcgtag cttatcgttg gatgatgtcg tcatactcgg atgggatttg
     1561 ccgttgtagg ttccgctgag aatctcgttg gccgagcgca tcttgtcttc cttcctgccg
     1621 ccagcgttcc aggatagcca taatgtagcg ccagcggcga gcgttattgg tgacggccag
     1681 cttgatggcc tcttcgatcc attgtggcgg gtaggtccgc tccgcctcgc gcagttcgtc
     1741 ggcgatgagc ggcgtcaatg cgccgatgtt ttgctcgtag agcacgaaga tgttcggccg
     1801 ttccaccatc agggtgattg gctgctcgtc gtcgggcgan nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnc gaagatgttc ggccgttcca ccatcagggt gattggctgc
     1981 tcgtcgtcgg gcgatggccg ccattcgccg cggctgatgc cctcgacggc cgcccgtccc
     2041 ctctcggtat tgataaagta aagcgccatc tggccgtcgg ctccttctac ggtcacctgc
     2101 aggaaggtgc cgcgagctac ggcccgttcg atgccgtcga gcagcgtgtc gacaccgctc
     2161 tggatgttcg gcccgcccat cgccttcagc aggatggggt cgttcatgaa atcgggtagc
     2221 cgcagatagc gcaccgcccc ctcgcgctgg cccaacgccc agaacgcgta gagcgtcacc
     2281 ttcagttcgg ccaggttgtc gatggccggc agcaggtcgc tgaagaacag attcggaacg
     2341 ggcgtcagcc gctgcttccc atcaggaaag ccgggaaagc cttgcatcga tgttgaacca
     2401 gccatgagaa cccttaatta attaagaatt aggaataatg aatcaggaat gaagagcgtg
     2461 tctttcgtac ttcgttcttc actcgtcact cgtcatttac aactcgatct cctgcttgtg
     2521 caggttgcgg aaggccgcca gtttgccgtg ccagaacagg tcgattgagc cggtggggcc
     2581 attgcggtgc ttggccacat tgatctcggc gatattcggc cgctcggatg tgtccgggtt
     2641 atagtagtcg tcgcgataga tgaacatgac gacgtcggcg tcttgctcga tggagttgtg
     2701 gacgacaatg tcgttggcga tgaagttgtg caggccgggc acggtcaggt caaagacctc
     2761 ttcgatggcg tccggttcga tcgagacgat caagtcccaa tagacgtcgc tctcggccag
     2821 cgcctgcaac tcggccgatt ccgcggcctg cgccactcgc gcggcccgct cgcggctaag
     2881 gttctggttc atgatggttg tgccgccata gggtgtgccg atgcccgcgt agagtgcccg
     2941 ctgcgtgaca ccggctgctt gcatggccgg acgcacgtgc gcgtcccata ccggctgggg
     3001 gattacatcc cggttggtat tctcttcccg ctcctcgata taggcgataa tctcatccag
     3061 tcgctcctcg cgagccggat tcacggctcc tatggagtgg gcaaagcgca gtacatcgcc
     3121 cttgccaccc acgacgatat ggtattgagc gcgtcccttg tcaccttgtg atacgcgctt
     3181 cagcatcccg ttgatcccca gccgcagcaa cagcgattgc acatcgcgcg ccaactgctc
     3241 gctgctggtt gcgtaataaa tcgccggccg ggggcttttt ccccaggtca tgcgcatgca
     3301 gccgtcggtg caccacaaat ggcgcaggaa cagggcgatg gcatgagatg gctgctggaa
     3361 tacgcgcccc ggcacgcgct tctcatatga gcgcaatccc catgccctca tagcgtcgag
     3421 ccattcgctg acggcgctgc gcgtgccgtg ggtatgatgc ctcgtagacg agagatacac
     3481 ctgataccag gtgcgctctt gctgaattcg cgggcgcact tccgagccga acaccgtggt
     3541 ggccagatca ctgaccatct cggccaggtc gctttccttg gtggtgtact ggatagcatg
     3601 gcgcggcaag gtgcagccgt cgccgatcag gtgacccagt agcgcaagtt cggcgtcgcc
     3661 catcgtttgc tcgtcggtgc ccgacattgc ccgcggcagg gcaatgtaga caccggccgc
     3721 cagttcgtcc agccgctccc agcccttgat ggtcatgaat agatggttgc ctgtggcacg
     3781 gattgtgcgc cccaaccggg ttgtcaggcg atacgtcggc ttggtccccg tcgaaaacgc
     3841 cttgctaacg acggcacgct ccagccgata agtcgtcgga ttcatggccc acacggcgaa
     3901 gtcggagcgg cccaccagat cgcgcatggg gatacgctcg cccgaatcgg ccagcgtcac
     3961 caacgtgtca ccggtcagac agcccgaatc gcgtaaatcc gacagcaccg gccgtttgtt
     4021 ctggcgctgc tccacagcgc ggctgagctg ggcggcggcc acgaccggta tgtccaactc
     4081 gcgggccaac cccttcagct cgcgcgaaat ctcgctgact tcctgcacgc ggttgttcgt
     4141 cgagcgttcg gccgacatga gttgcaggta gtcgatcatg atcaggtcca gcccatgctc
     4201 ggcatagagc cggcggcact tcgtccgcaa ctgcatgggg gtgatcgacg gcgtgtcgtc
     4261 gatgaagatg cgcgactccg acaggcggcc gagcgcctcc aggaagatgg cccactcatg
     4321 atcggccaaa tcgccgcgcc gcagccgctg ggaatcaatg cgcgtctcgg cggcgatcat
     4381 gcgctgtacc agctgctcgc ccgacatctc caggttgaac atggccaccc gcttgtcata
     4441 gcgacgggcg gcgttgagcg cgatggcgtt ctgcagcgcc gtcttgccca tgctgggccg
     4501 ggcggcaatg atgaccaggt ccgacttgtt cagcccgctc agcatccggt cgaggtcggt
     4561 gaagccggtg gggatgccga tgaactcgtc gccgcggtcg cgcagcagtt cgatacgctc
     4621 cagataatcg ccggcgatct ccttcagcgg cttcaggtcg cggttggtgc gcttctcgct
     4681 gatactgaac agggcatgtt cgctgcgatc gaggacgacg tcgatgtcct cggcctcgtt
     4741 ataggccagc ttggcgatgg ccccggcggc caggatcagg ttgcggcgag tggacgcggc
     4801 ctccaccatc ttgccgtagg cttcgacgtt gatcgaggtt ggcacggcat tgagcaggct
     4861 gatgaccgtc ggctcgccgc cgatctcgtc cagttgctcg cggcggcgca gctcgtcgat
     4921 gagagtgacg acgtccagcg gcacgcgccg ctcgttgagg gcgaggatgg cctcatagat
     4981 ccagcgattt tgcgcccgat agaaagcatc cgggcgcaag aaactggaca cttcaaatat
     5041 ggcgtcgggg tcgatcagca gcgaaccgag gacggcttcc tcggcttcga cgctatgggg
     5101 cggcaggcgt tcttcaatcg tatccattat gaatcaggaa tgtggggaat cgggaatcag
     5161 gagccggaag aagcgtcctc ttcaattcct gattcctgat tcgtaattcc taattgtcta
     5221 gggacgttcg tcgtcgccgc cggcgaagtc gtcgaaatcc agcgtgtcga ggaagtcgct
     5281 gaacgcatcg aacgggccga tgtcgtcggc cccttcggca tcatccgacc cctcctgcat
     5341 atcaggctcc ggctccaccc cggcgacatc catcacttct tcggagatga aaatcggcac
     5401 cttggcccgc acggccaggg cgatggcgtc ggacgggcga caatcgatct cgcgcggttc
     5461 gcgctcgccc tccatgttga tgaacaggcg ggcatggaag acgctctcgt tcagctcctt
     5521 gatcaggaca tgagagagcg tgccacccag ttcggtgatg acgtttttga gcaggtcctg
     5581 ggtcagcggc cgcgccactt tcacgtcttg cagctcgatg gtgatggcgt cggcctcgca
     5641 ggggccgatc cagataggta gctggcgctc gctgtccact tcacgcagga gcacaatgcg
     5701 gtgctgggac accaggctga tgcgaatgct ttcgatcata acctcgatca tgtacgatcc
     5761 tctctacttc gcttgtcgcg gcaatttgct cgacgttatt gtagcacaaa cgagccgtta
     5821 cgaaatacgc taacgaaacg ctgcggcggt gatcgccggg tctagatcgg cgaatagctc
     5881 gttgggaaca gcacccgctc ttcgattgtg ccgaccagat cgccgtggcg ggcggccgtc
     5941 tcgcgcttga tggccttcca ctcttcatcg gccaggaacg ccgcccaccc ggcgcgcatc
     6001 gttgcctcgt cgggccagga cagcagatag tagaactcag tgcgcccgtc cgtttgcgcc
     6061 tcccaaatgc cgagaatgtg gaagctgtaa gactgcatga tgcgccaggc atggtcgcgg
     6121 aaacgatcat gaaaggcggc cttgttgtgt tcgaaaatct cataaatacg cagttgatgg
     6181 atcacgactg tttacctcgt tcagaactgc ccgcaaaccg aggagttgta tgattcggcc
     6241 tgcggcgacg tcgtgtataa taacacggtc agggtggccg ccataaccgc gatgacgaag
     6301 gagagattgc cttgaaaccg atccattatg gagcgttgtt gatattgggc gccgtatggg
     6361 gcgcttcttt tttgtttatc ggcgtagccg tgcctgaatt cggcccgctg cggctgatgt
     6421 tcatccgggt gctcctcgcc gggctgattc tgttgggggt ggcccggctg accctgcgac
     6481 agaccagtct gcgtgatacg ttgcggctgc gggaaaactg gcgcaaatat ttgatcgtcg
     6541 gtctgctcaa ctccgctctg cccttcacgc tgatcgcttt ctccgaactt cggctcagcg
     6601 tctcgctggc cgccatcctg aactcgacga cgccactgtt cacggcgctg gtggccgcgg
     6661 cgtggggcag cgaacagctg accggccgca agatcgtggg cgtgctgttg ggcgtggttg
     6721 gggtgggggt gctggtgggc ggcgcgccgt tgacgctcga ccggggcatc ctcatggcgg
     6781 tgctggcctc gctgctggcg gccctcttct acggcatggg cgcggtctat gccgcgaggc
     6841 atatcagcag cttgccggcg gcctacgcct cgacgactca gctattggcg gcggctgtgt
     6901 ggctggccgt gccggcagcg gtggctccgg cgatccggcc atcgccgatg gctgttggcg
     6961 cgctggcggc gctgatcctc gtctccacca ccttcgccta cgtgctctac tttttcctgt
     7021 tgaaaaacat cggcccgacc cgcacggcca gcgtcacctt tctggtaccg gtattcggct
     7081 ccacgtgggc aatcatcttc ctgcatgagc cgttcagcct gagcatgctg ctggggctgg
     7141 caatcatcct gctcagcgtg gggctggtga ccggagcgcg ctttggacga ggtgctgcgg
     7201 ccgtgccgcc ggtggatgcc tgaacgaagt tctaatgtga aagaatcacc caatccgtcc
     7261 cgcgctgctc catcaccagc cgcaccagct tgatgtaggg ggcctgggtg gccatggcgt
     7321 aggcgacatt gccttgcagg tcaaccgtgt gcagctcgta gccatattcg gccgcggcgc
     7381 gcacgatatt gaggtagctg gtcgccatgg gatcgatgat ggccccgttg ttattgagaa
     7441 tgttgttcac actgtcgagg atgacgttgg cccggctgaa gtcgccgccg cgcagagccg
     7501 tgtcggccgc cgtcagcatc acttcgagca tgatgttgat ctcttcttcg gggcggcgcg
     7561 tcaggtcggc ggggttgccg aactcacgca cctgcgtcgg gtggggcagc caggcgctca
     7621 gataatgagc cgtggggtca tagatctcct gataatgccg ggccacgttg taatagcgaa
     7681 tcgtggtacg cagatcatcg acggccgccg ggtcgaccgg gacgctctgt agcgcggcca
     7741 tccattcggc ctcgatctgg gccagcgtcg cgccatagta gcgctgcaaa ttgacgtcga
     7801 ccgcgtcggc cagcgtcggc ccgtcgtcgg ccgagacctc gctgtagaaa tcacggaacg
     7861 tattccagcc gccacgctcg atgaggtagc tgacgaaccc ggccgcttcc agatagccaa
     7921 tctcgtgctg aacggggtag aaatcgctga tgagcgaggc caggggcacg tattggccca
     7981 gcgccagcag cgccgccgag cgttgcttga ggtcttctac tttataatgg ccgcccgtca
     8041 cccacaccgc cagcccttcg gccaggaagg agatgcgctg cggggcaaac tgctggtcga
     8101 ggatatgggc cgcctcatgg acgagcaact cgtagaaccc gccgccggca tagcgccgat
     8161 cgacgtagga caccaccata tcggtgccgg cgaacccgcc ctggccgacg atgcgatcga
     8221 tgaaatagac gtggagcttc tggctcggcg tcacgcccag acggttggcc gcctgggcca
     8281 cggcttgctc cagcagcgga gccagcgacc caaggtcgcg cgccgcggcc gtgtcgctga
     8341 cgacgtggag gacgcagcag ttggtctcca tcgtctccca ggtcgccgtc aggtcacgcg
     8401 ccgcctggcc ggtgggcaca tggactgtgg cgatgatgga gatggtgttg ttcgatgggt
     8461 cttcgtcgcc ggcctggatg gcgtcgcccg gatcgagcac gacttcgatg cggtaatcgc
     8521 cgctgaggcc ggtggtgtcc caggcccact catagacgcc ctcggcctgt ccgttccagt
     8581 tgcgccggtc gagcgggccg ccggccacct gcacgccgtc gaccagaatg gccgccatca
     8641 cctcgttgac gtggagatta tccggcacgt ggggcagcag ctggaaagtc accttgtcgc
     8701 cggcgtagat tttgggcacg gggtggagga tcaggtcggt gtagttgatg gccagatcgg
     8761 gcgcctcggt cgggtcggcc acggccgtcg gcgatggctc ggccgtaggt atggggagat
     8821 cggtcggcgg cagcgcaggc gcaagggtgt cggtcggcgc gacaggggcg atcgccgtat
     8881 cgacggcaac cgagggttcc gttgccgtcg actcgcttcc gatgacgacg ggtgcggcac
     8941 cggtcggccg attacaggcg acaagtccgg ttgcgaaaag agccaggatg acgagagcga
     9001 gcttctgcat cgctcctgat ggtatcaggc ggccgaacgt acaactcaac gcagattcaa
     9061 tccttactac acgactcata ttctatacgt caacgcgcgg cgaaaggttc cgggcaaccg
     9121 ttatgttcac ctattcctaa gctatagaga gtttctgttg cccttactca aaccggcaaa
     9181 acgatcgtat aatccgacgc gcatagtata gcatatcagg aggccctttg gaatggcctc
     9241 ccagacctga caggtgggtg ggcgaacgtt ccgtacagga acgagattcc gcgccaccca
     9301 cctgtcaggt ctctgatcac acactgcacg ctctataaat caatatgtca ggagaacatt
     9361 gaattatgac gttcaagaat attcaactgc ctgccggcga aaagattact tatgagaacg
     9421 gccggctcca tgtgccggat catccgatcg tcgccttcat cgagggtgac ggcatcggcg
     9481 tcgacatcac ccccgcttcc atgatggtat gggacgcggc tgtcgagaag gcttacggcg
     9541 gccggcgcaa gatcgcctgg atggagatct attccggtga gaaggcggcc gagaagtatg
     9601 acggcaacta catgcccgaa gagaccttcg acgccatgcg cgagtacatc gtcggcatca
     9661 aggggccatt gaccacaccc atcggcggtg gtttccgcag cctcaatgtg accctgcgcc
     9721 aggtgcttga tctctacgcc tgcgtgcgcc cggtgcgcta catccccggc acgcccagcc
     9781 cggtgaagca tccggagaag atgaatatcg tcatctatcg tgagaacacc gaggacgtct
     9841 actccggcgt cgaatgggaa gccaacaccg aagaggccaa ggcgattatc gagttcatga
     9901 acaccaaact gggcaagaat gtgcgcgaag ggtcggccat cggcatcaag ccggtcagcg
     9961 agttcggcag caaacggctg gtgcgccagg cgatccagta cgccatcgaa cacaatcggc
    10021 cgtcggtgac gtttgtccac aagggcaaca tcatgaagtt caccgaaggc gcgttcaaga
    10081 actggggcta cgaagtggcg gcgcaggagt ttggcgatta caccgtgacc gaggacgacc
    10141 tctggagcaa gtatgacggc aaggctcccg aaggcaagat cgtcatcaac gaccgcatcg
    10201 ccgacaatat gttgcagcag atgctgaccc gcaccgacga atatagcgtg ctggcgacgt
    10261 tgaacctgaa cggcgactac atcagcgatg cggccgcggc gcaggtgggt gggctgggca
    10321 tggcccccgg cggcaatatc ggcgacggcg tggccctgtt cgaggccacc catggcacgg
    10381 cccccaagta cgccggcaag gacatggtca accccggcag cctcatcctg agcggctcga
    10441 tgatgctgga gtacatgggc tggcaggaag cggccgacct ggtggagcag gcgatggcca
    10501 agaccatcca ggacaaggtc gtgacctacg acctgcaccg gcagatcgaa ggggcgac
//
LOCUS       DAOMQG010000090        10411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2753_length_10411_cov_10.583526, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000090 DAOMQG010000000
VERSION     DAOMQG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_10411_cov_10.583526"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1227
                     /locus_tag="PLC06_06925"
     CDS             22..1227
                     /locus_tag="PLC06_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRO90214.1"
                     /translation="MDKVIDELNRRVAESREDLLKFFFDIVAIPSMDSDIEAVGRRIG
                     EEMTRLGFDEVYVDRYGSIVGKIGSGPKILLYDSHIDTVGIGDPTQWPWDPFKGKIEN
                     GDFYARGALDEKGSTPGMVYGMAIARDMGLLDGYTAYYLGNIEEWCDGIGCQAFVDWE
                     GIKPDFVVIGEPTKMNVYRGHKGRLELEVICKGVSAHAASNFMGDNAIYKMLPVIAGI
                     RDSDATLPSHEFLGQGRITVTRISSVSPSDNAVPDECRIFIDRRVTFGETPESVIEAI
                     EALIPAETRKDFSVNRLVYDEPSHTGAVFVYDKYFPAWALAEADPFVVAGKDAIKALW
                     GRDDLAGKWDFSTNGNYWRGKAGIPSIGFGPGDEIYAHAVEEHVPLEDVVEATKFYAL
                     LPAMLSKNS"
     gene            1298..2272
                     /locus_tag="PLC06_06930"
     CDS             1298..2272
                     /locus_tag="PLC06_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRO90215.1"
                     /translation="MQTNLRGRDLITTQEWTKEEIDTLLDVAFDLKRKRALGQPHAYL
                     RDKVLGMLFFFSSTRTRVSFEAGMAQLGGHAEFIEATTTQISHGDTAKEIGEIIGRYT
                     DGIAIRQCDWDFGNKYIREVAAASRVPVLNMQCDVYHPFQILADLMTIMEKKGDPRGL
                     TINVSYAYASSYQKPISVPQSLILLMTRFGMNVRLSRPPEFKLMPEIMEQAKENARKS
                     RGSFEVLEDFDAGFKDADVIYAKSWGSMLTTSDHAESAKISQQYTDWITDERRMAMAK
                     EDAIYMHCLPADRNIEVTDGVLDGPQSVVYDEAENRLHVQKAAMALTM"
     gene            2386..3333
                     /gene="arcC"
                     /locus_tag="PLC06_06935"
     CDS             2386..3333
                     /gene="arcC"
                     /locus_tag="PLC06_06935"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRO90216.1"
                     /translation="MTGKLAVIAIGGNSLIKDKQHQSVEDQYQAADETAGHIARMIKD
                     GWNVAVAHGNGPQVGFILRRSELARHELHEVPLDVCGADSQGAIGYALQQSLQNHLLD
                     MGIDRPVATVVTQTEVSANDPAFRNPTKPIGSFMDEAEAMLRRERDKWDVVEDAGRGW
                     RRVVASPIPTRIVEEPSIRALIGAGHIVIGVGGGGIPVVADEQGHLKGIAAVIDKDFA
                     SSLLASRLGADTFIISTAVEKVALNFGKPNEQSLDRVTLAEAKAYLAEGTHFAKGSMA
                     PKIQAIVNFLEAGGSHAIVTDPPNIPRALAGETGTHFVR"
     gene            3461..>4338
                     /locus_tag="PLC06_06940"
     CDS             3461..>4338
                     /locus_tag="PLC06_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRO90217.1"
                     /translation="MNHILHLRSLISGRTFRTDEIDYVDPEYGPDGVVDVVYDYDAIK
                     RDISRESLRQSGERSMWRYKPLLPIEADAAVPPLQIGWTPIYPTPRLAADLGLRHLWV
                     KDDGRNPTASFKDRASAIAVVKGRERRAKIITTASTGNAAAALSGICASVGQANVIFV
                     PEKAPAAKVAQLLAYGATVMLVRGTYDDAVELCLKAADAYGWYNRTTGFNPYMTEGKK
                     TASLEICEQLGWQAPDRIFVSVGDGCIIGGLHKGLKDLMALGWIEKMPKLMGIQAAGS
                     NYLAEAWANNEDVLTKP"
     assembly_gap    4339..4438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4536..5000)
                     /locus_tag="PLC06_06945"
     CDS             complement(4536..5000)
                     /locus_tag="PLC06_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90218.1"
                     /translation="MGRRIITADALETMKGADGLESLEDAAIGTQRIDGFWDKVIKNI
                     PGDVVAGWTAILGLFGVSPGAVSGDANTTNILWVMWAALTVVTFFWTYRQTKMPSLQP
                     AWTQIFISTFAFVVWALALGVPFNTLSFYDSRVAAALLIIFTLGVGAVQPKE"
     gene            complement(5151..6392)
                     /locus_tag="PLC06_06950"
     CDS             complement(5151..6392)
                     /locus_tag="PLC06_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRO90219.1"
                     /translation="MADPFLSTNDRIKLAQTLERVYEMQLGPAARFGLLDKAGLRRFA
                     PGTDLAAAPSVLAGVLISKLEPYGMLPNQPGYHALGALVNYALIQPDTPQDDARFLAG
                     ILVKYELIADHGYLAELRTKYSLDVAPARQADPALLPPTEPPALVDEPEFDPVVGDQE
                     ALEQIVNSEDNFLDIDTLAGAIYSAQAVGRIELPKGRAHGTGFLVGPDLILTNYHVFK
                     SKDILPSAVIRFDYQANADGVVTPGRVFEFVPDFYIGSSDKELDFALVKVKGEPLAER
                     KMQPDDVGLDYLELLRRGRHRGYLLVSPSLIVEHERVNIIQHPNGNPQKAVLTQNYVL
                     STMSADRVHYLADTMPGSSGSPVLNNHWEVVALHHSGGAHPPQKATIDLQKILKGQYK
                     FNEGIPIKAILPLIERYLPRN"
     gene            complement(6431..7045)
                     /locus_tag="PLC06_06955"
     CDS             complement(6431..7045)
                     /locus_tag="PLC06_06955"
                     /inference="COORDINATES: protein motif:HMM:NF040089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90220.1"
                     /translation="MTPMPTELMLGLRDIFVTYFDLEGLTDLALALGIDFENLSGASK
                     GAKARELALYLWRHSKLPRLAEVGPAQRPDIDWSLLAPYAPNRIEPTTDPAQPAAGKL
                     DFTDIQRLLPVLAAYPLFQTPDGRNTILAISGVAPFVNLDLNGSAQSVAANLLVKLNE
                     YGAIAPDDTAIGRLLAYLVADATLPPDHKGIIAAIAAKYDLQIE"
     gene            7192..7965
                     /locus_tag="PLC06_06960"
     CDS             7192..7965
                     /locus_tag="PLC06_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90221.1"
                     /translation="MTSDNGRPNPIVQVRDVIKRFPVGDGEVTVLRGVSLDIYPGEFV
                     SLVGPSGNGKSTLLNMITGIDHPSEGEVIVTGQSVHMMSENELSVWRGEHLGIIFQFF
                     QLLPALSLIKNVMLPMDLVKKVPKRERPERAMTLLDMVGLADQAHKLPSQVSGGQQQR
                     AAIARALANDPPLIVADEPTGNLDSTTAEDVFQLFKGLIARGKTMIMVTHSLDLAVRG
                     SRVVEIRDGHITNDVPAALHPRARRSAGSNQLISESVGQ"
     gene            8019..>10411
                     /locus_tag="PLC06_06965"
     CDS             8019..>10411
                     /locus_tag="PLC06_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90222.1"
                     /translation="MGVLWDKVWFDLWQRKARTILAVLSITVGVLAIGAIFGLTDQLL
                     SGMNEAHQAVSPSHLNIIVRQFIDRDTAESLTDIPGVVGVEPMNMATVRYRTAPDAPW
                     QPATAVSRPDYTNQIYDWLSLKAGAWPDDNTVGVERITSEYYDIKMGDKLIFEMEGTD
                     RSFPVTGLIRHPFVPPPDFGGNAYFFFNQATMARFGFPEGQYVQLLVQVSPYSEAYAR
                     DRAAAIKEQLAKQGIGVSMIIYQEPEKHWGYDTVLGITIVLKILAIVSLLTSVIIVIN
                     TTMAIITQQTDQIGIIKSLGGTTRDVARLYLTGVLIFGLLALLVALPAGVLAAWLATK
                     QLLFIFNIDYDSFHFSAQAIVWQVLAAVLAPLLAALWPVLRGAAISVREALATYGLGG
                     DFGYSRVDRAVEHLAERLLPSPYTIALGNMFRRKGRLLLTQFVLVLAGAMFLMVLTLS
                     SSVTHTLDLELNRRQYDMRLFFFVHHRSDRIEALLEDYPGVAEAEAWYSVTGTVLREG
                     EEVEDTGGLGAELFGIPPGSTMYAPLIIDGRWLTPEDTDRVVVISRDTAEFNNVSVGD
                     TITINLGDLGEADFEVIGTYQAISPDVFSTDPIYAPAPAVVGVTRQANRANQVVIRTT
                     GDDIDSGTLLMQLDEYLNSYNMEVSPFFSRTRDQDREYAYNTFAIVNQILFSLAIVMG
                     IVGGIGLMGSLSISVVERTREVGVLRSIGGTSPVIMTMFILEGVLQGVMSWLLAVPLS
                     YLLARPLAAILGETIFKASLDFAYNYTAVAVWLVAVICIAFLASIIPAHAAGRISIR"
BASE COUNT         2061 a         3271 c         3068 g         1911 t
ORIGIN      
        1 cattaaacga gggaacattt tatggacaag gtaattgatg aactaaatcg acgcgtggcc
       61 gaatcccgcg aagacctgct gaaattcttc ttcgacatcg tcgccatccc cagcatggac
      121 agcgacatcg aggccgttgg caggcgcatc ggcgaagaga tgacccgcct cggcttcgat
      181 gaagtctatg tcgatcgcta tggctccatc gtcggcaaga tcggcagcgg ccccaaaatt
      241 ctgctctacg acagccacat cgacacggtg ggcatcggcg acccgaccca atggccgtgg
      301 gatccattca agggcaagat tgagaacggt gatttctacg cccgcggcgc gctcgacgag
      361 aagggcagca cgcccggcat ggtctatgga atggctatcg cccgcgatat gggccttctc
      421 gacggttaca ccgcctatta cctcggcaac atcgaggagt ggtgcgacgg catcggctgc
      481 caggccttcg tcgattggga aggcatcaag cctgatttcg tcgtcatcgg cgagccgacc
      541 aaaatgaacg tctatcgtgg ccacaagggc cgcctcgagc tggaagtcat ctgcaagggc
      601 gtcagcgccc acgccgccag caacttcatg ggcgataacg ccatctacaa gatgctgccc
      661 gtcatcgccg ggatccgcga cagcgacgcc accttgccga gccacgaatt tctgggccag
      721 gggcgcatca ccgtcacccg catctccagc gtctctccca gcgacaacgc cgtacccgac
      781 gagtgccgca tcttcatcga ccgccgcgtc acctttggcg agacgccgga atcggtcatc
      841 gaggccatcg aggcgctcat ccccgcagag acgcgcaagg acttttcggt caatcgtctg
      901 gtctacgacg aaccgagcca cactggcgcg gtcttcgtct atgataaata ctttcccgcc
      961 tgggcgctgg cagaggcaga cccgttcgtc gtggccggca aggatgccat caaggctttg
     1021 tggggtcgcg acgatctggc cggcaaatgg gacttctcga ccaacggcaa ctattggcgt
     1081 ggcaaggcgg gcatccccag catcggcttc ggccccggcg atgaaatcta cgcccacgcg
     1141 gtcgaagagc acgtgccgct ggaagatgtc gtcgaggcga cgaagttcta cgccctgctg
     1201 ccggctatgc tcagcaagaa ttcataggta cgcaccaata tatgcgccgg ccggtgacgg
     1261 ccggccaaca atccaaattg caagaggata aggaaacatg caaaccaatt tacgcggtcg
     1321 tgacctgatt accacgcaag agtggaccaa ggaagagatc gacaccctgc tcgatgtcgc
     1381 cttcgacctg aagcgcaagc gcgccctcgg ccagccccac gcctatctgc gcgacaaggt
     1441 gctgggcatg ctgttcttct tcagcagcac ccgcacccgc gtcagcttcg aggcgggcat
     1501 ggcccagctt ggcggccacg cggagttcat cgaggccact acaacgcaga tcagccacgg
     1561 cgacacggcc aaggagatcg gcgagatcat cggccgctac accgacggca tcgccattcg
     1621 ccaatgcgac tgggatttcg gcaacaagta catccgcgag gtcgcggccg cttcgcgcgt
     1681 gcccgtcctc aacatgcagt gcgacgtcta ccatcctttc cagattttgg ccgacctgat
     1741 gaccatcatg gagaagaagg gcgacccgcg cggcctgacc atcaacgtca gctacgccta
     1801 cgccagcagc taccagaagc ccatcagcgt gccccagagc cttatcctgc tgatgactcg
     1861 ctttgggatg aacgtgcgct tgtcccgtcc cccggagttc aagctgatgc cggagatcat
     1921 ggagcaagcc aaagagaacg ctcgcaagtc gcgcggttcg ttcgaggtgc ttgaggactt
     1981 cgacgccggg ttcaaggacg ccgacgtcat ctatgccaag agctggggct ccatgctgac
     2041 gacttccgat catgccgagt cggccaagat cagccagcag tacaccgact ggatcaccga
     2101 cgagcggcgc atggcgatgg ccaaggaaga cgccatctac atgcactgcc tgccggccga
     2161 ccgcaacatc gaagtgaccg acggtgtcct cgacggcccg caaagcgtcg tctatgacga
     2221 ggctgagaac cggctgcacg tgcagaaggc ggccatggcg ctgacgatgt agttggcgcg
     2281 tttaatgaga cggcagacca cggaccacag accacaaccg ttaccgggtt tgtctgtcgt
     2341 ccgtggtctg tcgtcggttg tccattatcc cccacggaga atcccatgac cggaaaactg
     2401 gccgtcatcg ccatcggcgg caactcgcta atcaaggaca aacaacacca aagcgtcgag
     2461 gatcagtatc aggcggccga cgagacggcg ggccatatcg cccggatgat caaggacggc
     2521 tggaatgtgg ccgtagccca cggcaacggc ccgcaagtcg gcttcatcct gcgccgctcc
     2581 gagttggctc gccacgaact gcatgaagtt ccgctcgacg tgtgcggggc agactcgcaa
     2641 ggggccatcg gctacgctct gcagcagagc ctgcaaaacc atcttctcga catgggcatc
     2701 gaccgcccgg tggcgactgt ggtcacccag accgaggtca gcgccaatga ccccgcgttc
     2761 aggaacccga ccaagccgat tggctcgttt atggacgagg ccgaggccat gctccggcgc
     2821 gagcgtgaca aatgggacgt ggtggaagac gccggccgtg gctggcggcg agttgtggcc
     2881 tcccccatcc cgacacgcat cgtcgaggaa ccgtcgattc gggcactgat cggcgcgggc
     2941 cacatcgtca tcggcgtcgg cggtggcggt atccccgtcg tggctgatga gcagggtcat
     3001 ttgaaaggca tcgccgccgt catcgacaag gacttcgcct cctcgctgct ggcgtcgcgg
     3061 ctgggcgcgg acacgttcat catcagcacg gccgtcgaaa aggtggcgct taactttggc
     3121 aaaccgaacg agcaatcgct cgaccgcgtg acgctggccg aagccaaggc ctatctggcc
     3181 gagggcaccc atttcgccaa gggcagcatg gcccccaaga ttcaggctat cgtcaacttc
     3241 ctggaagcag gcggctcgca cgccatcgtc accgacccac cgaacatccc ccgcgccctg
     3301 gccggcgaga cggggacgca ttttgtgagg taagcggtgg tcagtgggtc agtggggcag
     3361 taatcagtaa gtcagtaggc cagtaagcca gtgggtttgt ggatcagtaa aactgaatac
     3421 tgacttactg aatactgaca tcaggtgcca attatccaac atgaatcaca tactccacct
     3481 cagaagcctc atcagcggcc gaaccttccg cacggacgag attgactacg ttgaccccga
     3541 atacgggccg gacggcgtcg tcgatgtcgt ctatgactat gatgccatca agcgggacat
     3601 cagccgcgag agcctgcggc aaagcggcga gcgctccatg tggcgctaca aaccgctgct
     3661 gcccatagag gccgatgccg ctgtgccgcc gctgcaaatt ggctggacgc cgatctaccc
     3721 caccccgcga ttggccgccg atctgggtct gcgtcatcta tgggtgaaag acgacggccg
     3781 caacccgacc gcctcattca aggaccgcgc cagcgccatc gccgtcgtta aggggcgcga
     3841 gcgcagggca aagatcatca ccaccgccag caccggcaac gccgccgccg ccctcagcgg
     3901 tatctgcgcc agtgtcggcc aggccaacgt catcttcgtg ccggagaaag cgccggcggc
     3961 caaggtcgcc caattgctgg cctatggcgc gacagtcatg ctggtgcgcg gcacgtatga
     4021 cgatgcggtt gaactatgcc tgaaagcggc cgacgcttac ggctggtaca accgcacgac
     4081 cggcttcaac ccctacatga ccgaaggcaa gaaaacggca tcgctggaga tttgcgaaca
     4141 actcggctgg caggctcccg accgtatctt cgtcagcgtc ggtgacggct gcatcattgg
     4201 cggcctccac aagggcctaa aagacctcat ggccctgggc tggatcgaga agatgcccaa
     4261 actgatgggc attcaggccg cgggcagcaa ctatctggcc gaagcctggg cgaacaacga
     4321 ggacgtgttg accaagccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     4441 cgacctcgaa gacctgcgcc gcgtgatggc aggaatgtga cgcgtatcca gaaaccgagt
     4501 ttctcggaag aaactcggtt tctaatcttt ctaatctact ccttcggctg caccgctccc
     4561 acgcccagcg tgaagatgat cagcagcgcc gccgccacac gcgaatcata gaagctcaac
     4621 gtgttgaacg gcacgcccaa cgccagcgcc cagaccacga aggcgaacgt cgagatgaag
     4681 atttgcgtcc aggccggttg cagcgacggc atcttcgtct ggcgataggt ccagaagaaa
     4741 gtcacaaccg tcagcgccgc ccacatgacc cacaggatat tggtcgtgtt tgcgtcgccg
     4801 ctcactgctc cgggcgagac accaaacaga cccaggatag ccgtccatcc ggccacgacg
     4861 tcgccgggga tattcttgat caccttgtcc caaaagccgt cgatccgctg ggtgccaatg
     4921 gccgcgtcct ccagagactc caggccatcc gcgcccttca tggtttccag tgcatcggcg
     4981 gtgataattc gtcgtcccat aatgatgcct cctcagatga atgatgccgg tcggcgatga
     5041 agtcgctcgc ggctggtata ttgaatcaca attcgcatca atcgacaccg gaacgccgcg
     5101 gcgacgccgc ggaattcgcc ccgaacaacg taaaaagttc aacccgccgc ctaattgcgc
     5161 ggcagatagc gctcgatcag gggcagaatg gccttgatcg ggatgccctc gttgaactta
     5221 tactgcccct tcaggatctt ttgcagatcg atcgtcgctt tctgtggggg gtgcgcgccg
     5281 ccgctgtgat gcagggcgac cacctcccaa tggttgttga gcaccgggga gccggatgat
     5341 cccggcatcg tgtcggccag atagtgtacc cggtcggcgc tcatggtgct gaggacgtag
     5401 ttctgcgtca gcacggcctt ctgcgggtta ccgttgggat gctggatgat gttgacccgc
     5461 tcgtgctcga cgatcaagct gggcgaaaca agcagatagc cacgatggcg gccgcggcgc
     5521 agcagttcca ggtaatccag ccccacatca tccggctgca tcttccgctc ggccaacggt
     5581 tcccccttca ccttcaccag ggcgaagtcc agctccttgt cggagctgcc gatgtagaag
     5641 tcgggcacga actcgaacac gcgccccggc gtcaccaccc cgtcggcatt ggcctgataa
     5701 tcaaaacgga tgacggccga gggcagaata tccttcgact tgaacacatg gtaattggtc
     5761 aggatgagat cggggccgac gaggaaaccg gtgccgtggg cgcgcccttt cggcagttcg
     5821 atgcggccga cggcctgggc gctatagatg gctccggcca gcgtatcgat gtcgaggaag
     5881 ttgtcttcgg aattgacgat ctgctccagc gcttcctgat cgcccacaac cgggtcaaat
     5941 tcaggctcat cgacaagcgc gggaggctct gttggcggca atagtgccgg gtctgcctgg
     6001 cgtgccggcg ctacatcaag gctgtacttc gttcgaagct cggccagata gccgtggtcg
     6061 gcgattagct catacttcac cagaatcccc gccaggaacc gcgcgtcatc ctggggggtg
     6121 tcgggctgga tcagcgcata gttgaccagc gcacccaggg catggtagcc gggctgattg
     6181 ggcagcatgc cgtatggctc cagcttgctg atgagcacac ccgccagcac actgggagcc
     6241 gcagccagat cggtgccggg ggcgaagcgc cgcagcccgg ccttatccag cagcccgaag
     6301 cgagccgccg ggccgagctg catctcatag acgcgctcca gcgtctgagc gagcttgatg
     6361 cggtcattgg tgcttaaaaa tgggtcagcc atggtgtggt tcctgtgtgt ccagcgatgg
     6421 gaatgtccga ttactcgatt tgcagatcgt acttggcggc tatggcggcg atgatcccct
     6481 tgtggtcggg tggcaaggtg gcatcggcca ccaggtaggc caacaggcgg ccgatagccg
     6541 tatcatccgg cgcgatcgcg ccatactcgt tgagcttcac caacagattg gcggcgacgc
     6601 tctgggcact gccgttcagg tccagattga cgaaaggcgc gacccccgag atagccagga
     6661 tcgtgttgcg gccgtcgggt gtctggaaga gggggtaggc cgccagcacg ggcagcagcc
     6721 tctggatgtc ggtgaagtcg agcttgcccg cggccggctg cgcaggatcg gtcgtcggtt
     6781 caatccggtt gggcgcatag ggagccaaca ggctccagtc gatgtcgggg cgctgcgccg
     6841 gccccacttc agccagccgg ggtagcttgc tgtgtcgcca gagatacagg gccagttcac
     6901 gggctttggc gcctttgctc gcgccggaga gattctcgaa atcgattccc agcgccaggg
     6961 cgagatcggt caacccttcc aggtcgaaat aagtcacgaa gatatcccgc aggccgagca
     7021 tcaattcggt gggcatcggt gtcattcggc gtctcctcgc caaccggcga accattcaaa
     7081 gaaccgagcg tatcataaca taattctcgc cctgtcttca accgggatga caggattaga
     7141 atcaggtcgc gcttgtgatt ttcggcacaa atactgctaa acttatcgat catgaccagc
     7201 gacaacggac ggccgaaccc catcgtgcag gtgcgcgacg tgatcaagcg tttccccgtg
     7261 ggcgatggcg aagtcaccgt tctgcgcggc gtgtcgctcg acatctaccc cggcgaattc
     7321 gtttcgctgg tcggcccgtc gggcaacggc aagtcgactc tgctcaacat gatcaccggc
     7381 atcgaccacc ccagcgaggg tgaagtgatc gtcaccggcc agtcagtaca tatgatgagc
     7441 gagaacgagc tgtccgtctg gcgcggcgag catctgggca ttatcttcca gttcttccaa
     7501 cttctgcctg ccctcagcct gatcaagaac gtcatgctgc caatggatct ggtgaagaaa
     7561 gtgccgaagc gcgagcgacc agagcgagcc atgacgctgc tcgacatggt ggggctggcc
     7621 gaccaggccc acaagctgcc cagccaggta tcgggcggcc agcagcagcg cgccgccatc
     7681 gcccgcgccc tggccaatga cccgccgctg atcgtggccg acgaaccgac gggcaatctc
     7741 gactcgacga cggccgagga tgtgttccaa ttgttcaaag ggctgatcgc ccggggcaag
     7801 acgatgatta tggtgaccca tagtctcgat ctggccgtgc gtggttcgcg ggtcgtggag
     7861 atacgcgacg ggcatatcac caatgacgtc cccgccgccc tgcacccccg cgcccggcgg
     7921 tcggcaggca gtaatcagtt gatcagtgag tcagtaggtc agtagatcag tggacagtgg
     7981 ctgcctcttc attcgtaatt cgtaattcgt aatttcctat gggcgttttg tgggataaag
     8041 tctggttcga cctgtggcag cgcaaggcgc gcaccatcct ggccgttctc agcatcacgg
     8101 tgggtgtcct cgccattggc gcgatcttcg gcctgaccga ccaactgctg tcgggcatga
     8161 atgaggcaca tcaggccgtt tctccctcgc atctcaatat catcgtccgc caatttatcg
     8221 accgcgacac ggccgaatcg ctgaccgata ttcccggcgt cgtcggtgtc gagccgatga
     8281 acatggccac tgtgcgctac aggactgccc ccgacgcccc ctggcaaccg gccacggccg
     8341 tctctcgccc cgactatacg aatcagattt acgactggct gtccctcaag gccggtgcgt
     8401 ggcccgacga caacactgtc ggcgtcgaac gcatcaccag cgaatattac gacatcaaga
     8461 tgggcgacaa actcatcttc gagatggaag gcaccgaccg ctccttcccc gtcaccggcc
     8521 tgatccgcca tccgttcgtc ccgccgcccg acttcggcgg caacgcctac ttcttcttca
     8581 atcaggccac aatggcccgc ttcggcttcc ccgaaggcca atacgtccaa ctgttggtgc
     8641 aggtctcccc ctacagcgag gcctatgccc gtgaccgcgc ggcggccatc aaggagcagt
     8701 tggccaaaca gggcatcggc gtcagcatga tcatctatca ggagccggaa aaacattggg
     8761 ggtacgatac cgtgctcggc atcaccatcg tgctgaaaat cctggccatc gtctccctgc
     8821 tgaccagcgt catcatcgtc atcaacacga cgatggccat catcacccaa cagacggatc
     8881 agatcggcat catcaaatcg ctgggcggca cgacgcgcga cgtggcgcgt ctctatctga
     8941 ccggcgtgct catctttggg ctgctggctc tgttggtcgc cttgcccgcg ggcgtgctgg
     9001 ccgcctggct cgccaccaaa caactactgt tcatctttaa catcgattac gacagctttc
     9061 atttctcggc gcaggccatc gtgtggcagg tgctggccgc cgtcctggcc ccgctgctgg
     9121 cggcgctgtg gccggtgctg cgcggcgcgg ccatctccgt gcgcgaggcg ttggcaacct
     9181 acggcctggg cggcgatttc ggttattcgc gcgtcgatcg cgccgtggag catttggccg
     9241 agcggttgtt gccctcaccc tacaccatcg ccctgggcaa tatgtttcgc cgcaaggggc
     9301 ggttgctgct gacgcagttc gtcctcgtgc tggccggggc catgtttctc atggtgctga
     9361 ccctctcgtc ctcggtgacc cacacactgg acctggagct aaaccggcgg caatacgaca
     9421 tgcgcctgtt cttcttcgtc caccatcgct ccgatcgcat cgaggcgctg ctggaggatt
     9481 accccggcgt ggccgaggcc gaggcgtggt actccgtcac cggcacggta ttgcgcgagg
     9541 gcgaagaggt ggaggatacc ggcggtctgg gcgcggaact gttcggcatc ccgccgggca
     9601 gcacgatgta cgcgccgctg atcatcgacg gccgctggct cacaccggaa gataccgacc
     9661 gcgtcgtcgt catcagtcgc gacacggccg agttcaacaa cgtctccgtc ggcgacacga
     9721 tcaccatcaa tctgggcgac ctgggcgagg ccgacttcga ggtcatcggc acctatcagg
     9781 ccatctcccc cgacgtcttc tcgaccgacc ccatctacgc ccccgctccg gccgtggtcg
     9841 gcgtcacgcg gcaggccaac cgcgccaatc aggttgtcat ccgcaccaca ggggacgaca
     9901 tcgacagcgg cacattgctc atgcagctcg atgaatacct gaactcctat aacatggaag
     9961 tcagcccatt cttcagccgc acccgcgacc aggatcggga atacgcctac aacacattcg
    10021 ccatcgtcaa ccagattctg ttcagcctgg ccatcgtcat gggcatcgtc ggcggcatcg
    10081 gtctcatggg gtcgctgtcg atcagcgtcg tggagcgaac gcgcgaggtg ggcgtgctgc
    10141 gctctatcgg cggcacgtcg ccggtgatca tgaccatgtt cattctggag ggggtattgc
    10201 aaggggtgat gagctggctc ctggctgtgc cgctctctta tttgctggcc cggccgctcg
    10261 ccgccatcct gggcgaaacg atcttcaagg ccagcctcga cttcgcctac aactatacgg
    10321 ccgtagcggt gtggctggtg gccgtcatct gcatcgcctt cctggcatcg atcatccccg
    10381 cccacgccgc cggccgcatc agcatccgcg a
//
LOCUS       DAOMQG010000091        10404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2755_length_10404_cov_9.488260, whole genome shotgun sequence.
ACCESSION   DAOMQG010000091 DAOMQG010000000
VERSION     DAOMQG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2755_length_10404_cov_9.488260"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1463
                     /locus_tag="PLC06_06970"
     CDS             57..1463
                     /locus_tag="PLC06_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO90223.1"
                     /translation="MFRPLIRFSLLLMLLLGVAGCSLMEPQPQLIIPTPPAVAAADAP
                     TPGLLVPNEAGQLVTDPVSDIVPTVDPAIEALIAEVSQQQLMAYIRQVEGFGTRNAFS
                     DTQSESFGIGAARRWIFNEFVRVGNGRLTVEFQDFPLSYNGFSADQSNVVATLPGHGE
                     GNGIVVLMAHYDNRTPNVTDGVSRATGANDNGSGIALLLETARLMSSRDWNQTVVFLA
                     TSAEEQGSFGARYFAENAFLDGKNVIATLNYDSIGGRAGIPQHARLFAVDFLTSIHGA
                     LGRFYEYVGGLYLPTFPVVIYDALDREGRWGDHREFVRVGYPAIRVIESVEDPDLVNS
                     NRDTWDLIDYNYLQRMVQLNVAVAANLAGGPHQPDPPIIVPMAQPGSFMLTWPVTPDA
                     AGYAISFRPADSLYYPTFRFVKAGHAGNVALTGLDPSVTYAVSIAALNERGLMSGFSV
                     EQIVGPGISSSGETAAQP"
     gene            complement(1466..3106)
                     /locus_tag="PLC06_06975"
     CDS             complement(1466..3106)
                     /locus_tag="PLC06_06975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRO90224.1"
                     /translation="MRQRNTAVAALLFTLILTVLALSACGGPSTTTTSPAQATSPTIP
                     ATPEPTATPQPTATAEPTATIEPSATPPPTETPTPSPTPQTVTLVVAPEFEAETAAAL
                     VGVDDVSHRWQLIVEDDPAAILAEGRADLAIARGTEGTLVREEPLAFMVPFTYDWEQI
                     SAADAEAILANGHSLIRVVPWSAMTPDNKAMRVDGLHPTDPDYSYREYWSLVAAPGYE
                     EAAAALAPLLSDALAPLPTIRIAAVGDINYDRSPAYIIQTTGDLAYPLSRVAHIFEAA
                     DYTIANLETALGDVGEPAAKRYPFRSPPETAESLAIGGVDLVSLANNHAMDYGPEALL
                     QGIGLLHAAGVATVGGGADESAAHAPHIAEINGLKVAFLGYVNVTVEAITNFDTQSWS
                     ATADAPGLAWGDPARITADVAAIRPEVDLVVVILHSGYEYIEEPSEAQVAAARAAIDA
                     GADLVVGHHAHILQGIHRYNDGVIAYGLGNFLFNIDGPPETAILNVWLDQNGVRQLEL
                     LPAIIQEHGQPRLAEPEEARPILSRVYYLTTLLNAANP"
     gene            complement(3103..4032)
                     /locus_tag="PLC06_06980"
     CDS             complement(3103..4032)
                     /locus_tag="PLC06_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRO90225.1"
                     /translation="MPISGSRLTGLVVLFAVVLWIAGCAPTAASNPVVAITEIAATPR
                     PTARPPIVVTGLPPTATAEATAQAAPTISPTPLPPTAIPTATITPSPTPDRNVLCSRR
                     VPDNGLLTMITLTYGLSRDYEPAGLVDLSGYLPKEVTLGYPTQLRRMVINPLVDMIAD
                     MQAAGLKPAIISGYRSFSAQAIARQKWLEKEPDRANILSAPPGFSEHQLGTVVDFGSP
                     ELAGIVGQEDIEFHTWFYKTSEGIWLLENAHKYGFTLSYPFEAFETTGFFYEPWHFRY
                     VGVEMATFLHEQGISLTEHQLATEPPPCIPDSQ"
     gene            complement(4101..5183)
                     /locus_tag="PLC06_06985"
     CDS             complement(4101..5183)
                     /locus_tag="PLC06_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO90226.1"
                     /translation="MTKESLGYVELEWTCPFCNTRNPGRAKVCRNCGAAQPKDVQFHQ
                     AGEDKIVADQEGLEMAKGGPDVYCPFCGTRNLAGATKCARCGGDLTGAEQRESGQVLG
                     AAQTKPAPDIKCDYCGTLNPATNTKCKNCGSPLGLAAKSAATAPAVAPAKVSPTLMIV
                     GAIVMLLICGAIAFFFMRGASTESNVARVSSVNWKRSIVIMGYAPATLSTWADELPEG
                     AEVSYCSERQRGRSASPTENSEQVCGTPYLVDQGTGFGEMVQDCEYIVYDDYCEYTIM
                     QLQPVGVMDETGSDLNPFWPETRLETGQELGERSQTYQITFDINGEPVVYQTTNPDEY
                     LQFQPNSQWTVEVNGFGRIVDIRPAP"
     gene            complement(5233..6993)
                     /locus_tag="PLC06_06990"
     CDS             complement(5233..6993)
                     /locus_tag="PLC06_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRO90227.1"
                     /translation="MDSATLTIFNHLFASVAEEMGVTLGRTAYSPNIKERLDYSCAVF
                     LGGEHPAMLAQAAHIPVHLGAMPASVRAAVERCAPFHPGDVVILNDPYLGGNHLPDIT
                     LVSPVFVSNQYPVDQYPVDQCSVNQTTVPLLTDPLLTDLLLTDLLPTDLLPTGPLPTA
                     PRPHFLVASRAHHADVGGMSPGSMPLSTEIYQEGIIIPPIKLVDRGKRNEAVWQLILR
                     NVRTPWERDGDLAAQLAAHATGAARLAEIAERYGLDETLRQAHALIDYTESLTRAAIR
                     MIPNGRYVFTDYLDDDGQSDEPVPICATVDIADDSLAVDFAGTAAAVAGNLNAVPAIV
                     DSAVAYCLRCVALALLDADLPMNEGAFAPLAVHIPPGSLLDPQPPHAVAAGNVETSQR
                     VVDVVFGALAQALPDLIPAAGQGTMNNLTFGGAYPAGWSSASTQFAYYETIGGGAGAG
                     PSSDGASGVHVHMSNTRNTPIEALEYSFPIRVETYSLRRDSGGRGLHHGGDGLVRTIR
                     FLAPATVTITAERRRRGPYGLAGGQAGAPGGNKLWHNRDVVELPGKTTFSVGSGDVVE
                     IETPGGGGWGESTKKISKDL"
     gene            complement(7065..7613)
                     /locus_tag="PLC06_06995"
     CDS             complement(7065..7613)
                     /locus_tag="PLC06_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRO90228.1"
                     /translation="MSQRDTYTVEVAGLVRHFPLFEVAPGVRIAVFNMLGDTYVVKAA
                     AAALAEKLKELHADVLVTAEAKSIPLIYEMSALMGLPYVVMRKAYKSYMGEAISAETV
                     SITTGAPQTLFLDEKDRALVKGKRVIVIDDVISTGSTLNGMEKLVLKAGGEVAQVAAV
                     FTEGDDDWSRVVALGNLPVFRN"
     gene            7723..8700
                     /locus_tag="PLC06_07000"
     CDS             7723..8700
                     /locus_tag="PLC06_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRO90229.1"
                     /translation="MKAIVVHGEKNAPRLVWEDVADIGYGEDEALVDVRATAVNRADL
                     SQARGNYPPPPGASHILGLEMAGVIRAVGANVVGWRPGDRVCALLPGGGYAGQVAVPA
                     GMLLRLPDNWSFAQGAAVPEVWYTAYINLFDEGQLKAGETALIHAGASGVGTAAIQLA
                     LDAGARAIATAGSEEKVAFCRELGALAINYKTQDFLEQVMVVTDGKGVDVILDPVGGS
                     YLASNVAALRRFGRLVNIGLLGGAKGELNMGQLLGKRLHIVGSTLRTRPVEEKIAITR
                     RFAAEIMPRLADGRLRPIIDTTFPIAEAQAAHEYVLANRNTGKVILGVG"
     gene            8798..>10404
                     /locus_tag="PLC06_07005"
     CDS             8798..>10404
                     /locus_tag="PLC06_07005"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRO90230.1"
                     /translation="MRLSRSFGKTLREAPAEAELISHQLLIRANFIRPAGAGIYSFMP
                     FGYRVIRKIWDIMAEEMNAIDGQEMWMPNLHPAALWQATGRWGQVDVLFKLKGSGNRE
                     YALSATHEEIVVDLALRDIESYRDLPKMVYHISKKFRDEPRPRGGLIRLREFIMKDAY
                     TLDRSEQALDEYYPDMIQAYYNIFNRCGVPTVAINADVGAMGGKTSQEFTVPHPQGED
                     LFIQCNNCDYAANVEAAEFVREGEKPVSLAELVKVETPDCKTIADVAAFVGVPTSQTL
                     KCVFYWWRPSYVEKPGEGRLVFALVRGDLDINDTKLVNAVGGGFLRAATEEEIRAIGA
                     VPGYASGIGMAPAKDLTSPGVMIVADESIHYGGNYVVGANEDPWHYTGANYGRDFSVS
                     KIADIAQAATGHKCPQCGGQIEAKRAIEVGHCFKLGTRYSAAVNATYLDEDGQPQLIY
                     MGSYGIGLDRLMAVIVELHNDKDGIIWPDSVAPYQVHLVHIGKEGDGTKEKAEALYAD
                     LTAAGVEVLYDDRDGLGAGVKFKDADL"
BASE COUNT         1905 a         2991 c         3442 g         2066 t
ORIGIN      
        1 ttgattggtt atccttgttg attgtcagcg aacaggagaa tccgtaaagg tagtcagtgt
       61 ttagaccgtt aatccgtttt tctctgcttt tgatgctttt gttgggtgtg gcgggctgta
      121 gcctgatgga gccgcagccc cagctgatca tccctacccc accggctgta gccgcagccg
      181 atgcgcctac tcccggcctt ctggtgccca acgaggctgg acagcttgtc accgatccgg
      241 tgagcgatat cgtgccgacg gtggacccgg caatcgaagc cttgatcgcc gaagtatccc
      301 agcaacaact gatggcttat attcggcagg tcgagggctt tggaacgcgc aacgccttca
      361 gcgacaccca atcggaatca ttcggcattg gcgcggctcg ccgctggata tttaacgagt
      421 ttgtgcgcgt tgggaatggg cggctgacgg tcgagttcca ggacttcccc ctgagctata
      481 acggtttttc ggccgatcaa agcaatgtgg tcgccacgct gccagggcac ggagaaggca
      541 acggcattgt ggtgctgatg gcgcattatg ataaccgcac gcccaatgta accgacggcg
      601 ttagccgggc cacgggcgcc aacgacaatg gctcaggcat cgcgctcctg ctcgagacgg
      661 cgcggcttat gagttcgcgt gactggaacc agacggtggt ctttttggcg acgtcggccg
      721 aggagcaggg ttcttttgga gcgcgctact tcgccgaaaa tgccttcctt gacggcaaaa
      781 atgtgatcgc cacgcttaac tacgactcga ttggcgggcg agccggtatt ccccagcacg
      841 cgcggctctt cgcggttgat ttcctgacct cgattcatgg ggcgctcggc cgcttctatg
      901 agtacgttgg cgggctgtat ttgcccacgt ttcctgtcgt catctatgat gctctggacc
      961 gcgagggacg ctggggcgat catcgggagt tcgtgcgcgt cggctatccg gcaattcgtg
     1021 tgattgaatc ggtcgaggat cccgatctgg tcaactccaa tcgggatacg tgggatttaa
     1081 tcgactacaa ttatttgcag cgcatggtgc aattgaatgt ggccgtggcc gccaatttgg
     1141 ccggcggccc gcaccagccg gatccgccta tcattgtccc aatggcccag ccgggttctt
     1201 tcatgctgac ctggccggtg actcccgatg ccgccggcta cgcgatctcc ttccggcctg
     1261 ccgattcgct ctactacccg acctttcgct ttgtgaaagc cggccatgcc ggaaatgttg
     1321 ccctgactgg attggacccc agcgtcacct atgcggtcag catcgctgct ctgaacgagc
     1381 gcggcctgat gagcggcttt tcagtggagc agattgttgg gccgggtatc tcgtcgtccg
     1441 gagaaacggc cgcgcagcca tgaccttacg ggttggcggc gttcagcagc gtggtcagat
     1501 aatacactct ggacaggatc ggcctggctt cttcgggctc ggccaaacga ggctgaccgt
     1561 gctcctggat gatcgccggt aatagttcca attgacgcac gccgttttgg tcgagccaaa
     1621 cgttcaggat tgctgtttcc ggcggcccgt cgatgttgaa caggaagttc cccaagccat
     1681 aagcgatcac cccgtcgttg tagcggtgga tgccttgcag gatatgggcg tgatgaccca
     1741 cgacgagatc ggccccggcg tcaatcgcgg cccgcgccgc ggccacctgt gcctcactgg
     1801 gttcctcgat gtattcatac ccgctatgca ggatgacaac caccagatcg acctcaggcc
     1861 tgatggccgc tacatcggca gtgatacgtg ctgggtcgcc ccaggccaga ccgggggcgt
     1921 cggccgtcgc cgaccaactt tgcgtgtcga aattggtgat agcttcgaca gtgacattga
     1981 cataaccgag gaaagctacc ttcaggccgt tgatctcggc aatgtggggc gcatgggcgg
     2041 ccgattcatc cgcgccgccg ccgaccgtgg ccacgcccgc ggcgtggagc aggccgatac
     2101 cctgcaggag cgcctccggc ccgtaatcca tcgcgtgatt gttggccagc gacacgagat
     2161 cgacgccgcc aatggccagc gattcggccg tttccggcgg cgaacgaaac ggatagcgct
     2221 tggccgccgg ttcgcccacg tcacccaaag ccgtctccag gttggcgatg gtatagtcgg
     2281 ccgcttcgaa gatgtgcgcg acgcgagaga gcgggtaggc cagatcgccg gtggtctgga
     2341 tgatataggc gggggagcga tcgtagttga tgtcgccgac cgccgcgatg cgaatggtgg
     2401 gcagcggcgc taacgcatcc gaaaggagtg gggccagcgc ggcagcggct tcttcataac
     2461 ccggcgcagc gacaagcgac cagtattcgc ggtaggagta atcggggtct gtgggatgca
     2521 ggccatccac ccgcatggcc ttgttgtcgg gcgtcatcgc gctccagggc acgacgcgga
     2581 tcaggctgtg gccgttggcc aggatggctt cggcatcggc cgcgctgatt tgttcccaat
     2641 cgtatgtgaa gggcaccatg aaggccaatg gttcctcgcg gacgagggtt ccttctgtac
     2701 cgcgggctat cgccagatcg gcccggcctt cggccaatat tgccgccgga tcatcctcga
     2761 cgatcagttg ccatctatga gacacgtcgt cgacccccac cagcgcggcg gccgtctctg
     2821 cttcgaattc gggtgcaacg accagcgtga cggtttgagg cgttggggaa ggcgttggtg
     2881 tttcagtcgg gggcggggtg gcgctgggtt cgattgtggc tgttggttcg gccgtggccg
     2941 tcggttgagg tgtggcagtt ggctcaggcg ttgccggaat ggtcggagag gtggcctgtg
     3001 ccggcgatgt cgtcgttgta gatgggccgc cgcacgcgct gagcgcgaga acggtcagga
     3061 tcagggtgaa gagaagggcc gcgacggccg tatttcgttg tctcattgag aatcggggat
     3121 gcaggggggc ggttcggtcg ccaactggtg ttcggttagc gagatgccct gttcatggag
     3181 gaacgtggcc atctcgacgc cgacgtagcg gaaatgccat ggctcataga aaaagccggt
     3241 cgtctcgaac gcctcgaatg ggtagctgag cgtgaagcca tacttgtgcg cgttttccag
     3301 cagccagatg ccctcgctcg tcttgtagaa ccaggtgtgg aattcgatgt cttcctggcc
     3361 cacgatgccg gccagttcgg gcgagccgaa atccacgacc gtgcccaatt ggtgctcgct
     3421 gaagccgggc ggagcgctga ggatattggc tcgatccggt tccttttcca gccatttctg
     3481 gcgcgcgata gcctgggccg agaagctgcg gtagccggag atgatggccg gcttaagtcc
     3541 cgcagcctgc atgtcggcga tcatgtccac cagcgggtta atgaccatgc gtcgtaattg
     3601 cgtgggatac cccagcgtga cttccttggg caggtaaccg gataaatcca ccaggccggc
     3661 cggttcgtaa tcacggctga ggccataggt cagggtgatc atggtgagca gaccgttatc
     3721 cggcacgcgc cggctgcaga gcacgttgcg gtcgggcgtc ggggacggcg tgatcgtggc
     3781 ggtcgggatc gccgtgggag gcagcggtgt cgggctgatg gtgggggcag cttgggccgt
     3841 tgcttcggct gtcgccgttg gtgggaggcc tgtgacgacg atcggcggcc gagcagtcgg
     3901 tcggggtgtg gcggcgatct cggtgatagc gacgaccgga ttggatgcag cggtgggcgc
     3961 acaaccggcg atccatagca ccacggcaaa caggacgaca aggccggtca accggcttcc
     4021 tgaaatcggc atgatgcctt tcaatatatc ggagatcggc gccaccggcc actgccctaa
     4081 ggatgagtga tgaggtccgg tcagggcgcg ggtcgaatgt cgacgatccg gccgaacccg
     4141 ttgacctcta ctgtccattg actgttgggt tgaaattgca ggtactcatc cgggttcgtc
     4201 gtctgataga caaccggctc gccgttgata tcgaaggtaa tctggtaggt ttggcttcgc
     4261 tcgcccaatt cctgaccggt ttccagcctc gtttcgggcc agaacgggtt gaggtcggag
     4321 ccggtctcat ccatgacacc caccggctgt aattgcatga tggtgtattc acaataatcg
     4381 tcatagacaa tatattcgca atcctgcacc atctcgccga agcccgtccc ctgatcaacc
     4441 agatagggcg tgccacagac ctgttccgag ttttccgtgg gggaggcgga acggccgcgc
     4501 tggcgctcgc tgcagtagct gacctcggcg ccttcgggta attcgtcggc ccacgtgctg
     4561 agggtggcgg gggcgtagcc catgatgacg atgctgcgct tccagttgac gctgctgact
     4621 cgcgcgacgt tgctttcagt gctcgcgccg cgcatgaaga aaaaggcgat cgcgccgcag
     4681 atgagcagca tgacgattgc gccgacgatc atcagggtcg ggctgacttt ggccggagca
     4741 acggccgggg cagttgcggc cgactttgcc gccaaaccta acggagagcc gcaatttttg
     4801 catttcgtgt tggtggccgg gttgagcgtg ccgcaatagt cacatttaat gtcaggcgcg
     4861 ggtttggtct gggcggcgcc gagcacctgg ccgctctcgc gttgctcggc gccggtcaga
     4921 tcgccaccac agcgggcgca cttggtcgcg ccggcgaggt tgcgtgtgcc gcaaaaagga
     4981 caatagacat ccgggccgcc tttggccatc tccagccctt cctgatcggc gacgatcttg
     5041 tcttcgccgg cctgatggaa ctggacatcc tttggctggg ccgcgccgca attgcgacag
     5101 actttcgctc gccctggatt gcgggtgttg cagaaggggc aagtccactc gagttctaca
     5161 tagcccagag attctttcgt catacgaccg cttctccgat acgaggatta actcccgatt
     5221 gtatcacgtt cctcaaaggt ctttcgagat ttttttggtt gattcacccc atcctccgcc
     5281 gccgggtgtc tcgatctcca ctacgtctcc cgacccgacg ctgaaggttg ttttgccggg
     5341 tagctctact acatcacgat tatgccacag cttgttgccg ccgggcgcgc ctgcttgccc
     5401 cccggccagg ccataggggc cacgtcgccg ccgttcggca gtgatcgtca ccgtggccgg
     5461 agcgagaaaa cgaatggttc gcacgaggcc gtcgccgccg tggtgaaggc ctcggccgcc
     5521 ggagtcccgg cgcaagctgt aggtttcgac gcggatcggg aagctatatt ccagcgcctc
     5581 aatgggcgtg ttgcgcgtgt tgctcatgtg gacgtggacg cccgacgcac catccgacga
     5641 cggcccggct cccgcgccgc cgccgatggt ctcgtagtag gcgaattggg tggacgcgga
     5701 tgaccacccg gccggatagg cgccgccgaa ggtcaggttg ttcatcgtgc cctgaccggc
     5761 agcgggtatc agatcgggga gggcctgggc cagcgcgccg aagacgacat cgaccacgcg
     5821 ttgcgatgtc tcgacgttgc cggcagctac ggcgtggggc ggctgcgggt cgagcagaga
     5881 gccgggcggg atatgcacgg ccagcggcgc gaaggccccc tcgttcatcg gcaggtcggc
     5941 gtcgagcagg gccagagcga cacagcgcag gcaataggcc acggccgaat cgacgatggc
     6001 tggaacggca ttgagattac cggccacggc tgccgctgtc ccggcaaaat caacggccag
     6061 gctgtcgtct gcaatatcta ccgttgcaca aatgggcacc ggttcatcgc tttggccgtc
     6121 gtcgtcgaga tagtcggtga aaacgtaacg gccgttgggg atcatgcgga tagcggcgcg
     6181 ggtcagcgat tcggtgtaat cgatgagggc gtgggcctgt cgcagcgtct catccaggcc
     6241 atagcgctct gcgatctcgg ccagccgcgc cgcgccggtg gcgtgggccg ccagctgcgc
     6301 cgccaggtcg ccgtcgcgct cccacggagt gcgcacgttg cgcaggatga gctgccagac
     6361 cgcttcgttg cgcttgccgc gatccaccag cttaatcggc gggatgatga tcccttcctg
     6421 atatatctct gtcgaaagag gcattgaccc tggggacatg ccgcccacgt cggcgtggtg
     6481 ggcgcggctg gcgacgagga agtggggtcg tggagcagtg ggcagtgggc cagtaggcag
     6541 tagatcagta ggcagtagat cagtaagcag tagatcagta agcagtgggt cggtaagcag
     6601 tgggacagtt gtttggttta ccgaacactg atctactgga tactgatcta ccggatactg
     6661 attactgacg aagaccggcg acaccagggt gatgtccggc aggtggttgc cgccaaggta
     6721 ggggtcattg aggatgacga cgtcgccggg gtggaagggg gcgcagcgct cgacggccgc
     6781 gcgcaccgag gccggcatcg ctcccagatg gacggggata tgggccgcct gggccagcat
     6841 cgccggatgc tcgccaccca ggaaaacggc gcagctgtag tcgagtcgct ccttgatgtt
     6901 ggggctatag gctgtgcggc cgagcgtgac gcccatctcc tcggcaaccg aggcgaaaag
     6961 gtggttaaag atggtcagcg tggctgaatc catgaagtga cgaatgacga gtgacgagtt
     7021 cagaaacaaa ctgcagacaa tgagtgatgg tctgtcgtca gtggtcagtt tctaaatacc
     7081 ggcaaattac caagcgccac cacccgcgac cagtcatcat cgccctcggt gaagacggcg
     7141 gcgacctgtg cgacttcgcc cccggctttg aggaccagct tctccatgcc gttgagcgtg
     7201 ctgccggtgc tgatgacgtc gtcgatgacg atgacgcgct tgcccttgac gagcgcgcgg
     7261 tctttctcgt cgaggaagag cgtttgcggt gcgccggtgg tgatcgacac cgtctcggcg
     7321 ctgatcgctt cgcccatgta gctcttgtac gctttgcgca tgacgacgta gggcaggccc
     7381 atcagggcgc tcatctcgta gatgagggga atgctcttgg cctcggccgt caccagcacg
     7441 tcggcgtgca gttccttgag cttctcggcc agggcagccg cggcggcctt cacgacatag
     7501 gtatcgccga gcatgttaaa cacggcgatg cgcacgccgg gggcgacctc gaacagtggg
     7561 aagtgacgga cgaggccagc cacttcaacc gtataggtgt ctcgttggct catgatcgaa
     7621 attctcctgc tttcatagtc acggccgctc actggagcga tcgcacgagt gccgatagaa
     7681 tgaagtttat cgcaggagac gaccctgaca aggagtatta ctatgaaggc gattgtggtg
     7741 catggcgaga agaacgcgcc ccggctggta tgggaagacg tggctgatat aggttatggg
     7801 gaagatgagg cgctggttga tgtgcgggcg acggccgtca atcgggccga cctgtcgcag
     7861 gcacggggca actatccgcc gccgcccggc gcgagccaca tcctgggtct ggagatggcc
     7921 ggggtgatcc gcgcggtcgg cgcaaacgtc gtcggttggc ggccgggcga tcgggtatgc
     7981 gccttgttgc cgggtggtgg gtatgccggg caggtggccg tgcccgccgg gatgctgctg
     8041 cgcttgccgg ataactggtc gttcgcgcag ggcgcggccg tgccggaagt gtggtatacc
     8101 gcctatatca acctcttcga tgaaggccaa ttgaaggcgg gcgagacggc gctcatccat
     8161 gccggggcca gcggcgtggg cacggcggcc atccagttgg cgctggatgc cggggcgcgg
     8221 gccatcgcca cggccggcag tgaggagaag gtcgccttct gccgcgagct gggcgcattg
     8281 gcgatcaact acaagacgca ggacttcctg gagcaggtga tggttgtgac ggatgggaaa
     8341 ggcgttgatg tcatactgga tcccgtcggc ggatcatatc tggcgagcaa cgttgcggcg
     8401 ttgcgccgct tcgggcggct ggtcaatatt ggcctgctcg gcggggcaaa aggagagctg
     8461 aatatggggc aactgctcgg caagcgactg cacatcgttg gctccacgct tcggacgcgg
     8521 ccggtcgagg agaagatcgc catcacccgt cgcttcgcgg ccgagatcat gcccaggttg
     8581 gccgatggac gcctgcggcc gattatcgac acgacgttcc ccatcgccga ggctcaggcg
     8641 gcgcatgagt atgtgctggc aaaccggaat acgggaaagg ttattcttgg ggtaggatga
     8701 gaaggggaga aagggggaaa gggagagagg gtgaaggagg tggcccggct tgctgcgcgt
     8761 ttggcgctct ttggccccag agatatactc cgccgtcatg cgactttcac gatccttcgg
     8821 taagacactg cgcgaggccc cggccgaggc cgagctgatc agtcatcaac tgctgattcg
     8881 agccaacttt attcgtcccg ccggagcggg tatctactcc ttcatgccgt tcggctaccg
     8941 cgtcatccgc aagatatggg atatcatggc cgaggaaatg aacgccatcg acggccagga
     9001 aatgtggatg cccaacttgc acccggcggc gctctggcag gccacgggcc gttggggcca
     9061 ggtggatgtg ctcttcaagc tgaagggcag cggcaaccgc gaatatgcct tgtcggcaac
     9121 gcatgaagag atcgtcgtcg atctggctct gcgcgacatt gagagctatc gcgatctgcc
     9181 gaagatggtt taccacatca gcaagaaatt ccgcgacgaa ccacggccgc gcggcggcct
     9241 gattcgcctg cgcgaattca tcatgaagga cgcctacacg ctcgaccgca gcgagcaagc
     9301 cctggacgag tattatcccg acatgatcca ggcctactac aacatcttta accgttgtgg
     9361 cgtgccgaca gtggccatta acgccgacgt cggcgcgatg ggtggaaaga cctcgcagga
     9421 gttcaccgtc ccccatccgc agggtgaaga cctcttcatc cagtgcaaca actgcgacta
     9481 tgccgccaac gtcgaggcgg ccgagttcgt tcgcgagggc gagaagcccg tctcgctcgc
     9541 cgagctggtc aaggtcgaga cgccggattg caagaccatc gccgacgtgg ccgctttcgt
     9601 cggtgtgccg accagtcaaa cgctcaaatg cgtcttctac tggtggcggc cgagctatgt
     9661 ggagaaaccg ggcgaagggc ggctggtgtt cgctctggtg cgcggcgacc tcgacatcaa
     9721 cgacaccaag ctggttaacg cggttggcgg tggctttctg cgcgccgcga ccgaggaaga
     9781 gattcgggcc attggtgccg tgcccggtta cgcttcgggc atcggcatgg cgccggccaa
     9841 agatttgact tcgccgggcg tcatgattgt cgccgatgaa tcgatccact atggcggcaa
     9901 ctacgtcgtc ggggccaatg aagatccctg gcactacacc ggcgcgaatt acggccgcga
     9961 cttctccgtc agcaaaatcg ctgatatcgc ccaggctgcc acggggcaca agtgccccca
    10021 atgcggcggc caaatcgagg ccaagcgagc gatcgaggtc ggccactgct tcaagctcgg
    10081 cacccgctac agcgccgccg tcaacgccac ctatctggac gaagacggcc agccgcagct
    10141 catctacatg ggcagctacg gcattggtct cgatcggttg atggcggtca ttgtggaact
    10201 ccacaacgac aaggacggca tcatctggcc cgacagcgtc gcgccttatc aggtgcatct
    10261 ggtccacatc ggcaaggaag gcgacggcac gaaggaaaag gccgaggcgc tctatgccga
    10321 cttgaccgcc gccggcgttg aggtgctcta cgacgaccgc gacggcctgg gggcgggtgt
    10381 caagttcaag gacgccgacc tgat
//
LOCUS       DAOMQG010000092        10370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2765_length_10370_cov_10.290160, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000092 DAOMQG010000000
VERSION     DAOMQG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2765_length_10370_cov_10.290160"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..852)
                     /locus_tag="PLC06_07010"
     CDS             complement(226..852)
                     /locus_tag="PLC06_07010"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRO90231.1"
                     /translation="MPFAWWALVGLFVALLINRAADCWLGPARLQCGLTRHPRRQMVV
                     LIALPILFIWLAWRAPASGDPWVAASFAAILILLAVIDLEQRRVPNGVVLPATLFALG
                     IAWQSGELPSAVGGAVLAFVVFLALYALGRRLYGPGALGMGDVKLAGLIGAMVGLAWL
                     PLALLLGVLLAGVAAAALLLSGRAGRGDLLPYGSFMTLAAVIVLIAIN"
     gene            958..3483
                     /locus_tag="PLC06_07015"
     CDS             958..3483
                     /locus_tag="PLC06_07015"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRO90232.1"
                     /translation="MNRTTRILLIILGVLAGLALVMGIALASITRSPFPDIDGEKKMA
                     LQEACRNAAGEQNAICTALVGHGLKSPVHIFRDANGIPNIYAENTEDLYFAQGYVHAQ
                     DRMWQMEFWRHIAQGRVSEIVGEPGIENDKFIRTSGWNRITQANIDYYEKELPEAMDA
                     LNAYSAGVNAYLAEKSGDMAISQRVMGLVGEPWEIEPWQPADSIGWGVVMAWDLGGNM
                     SEERTRAKFYSLIGEEATDELLPGYPYDTRPVIAPTADLISAATGGNKAAAAPAIDWA
                     RIPTDIIGEPPVTAFGTGPDLGSNNWVVSGEHTETGLPLLANDPHLSIQMPSIWYQVG
                     LHAPGMDVIGFSFAGIPGVIVGHNNRIAWGVTNVGPDVQDLYIEKINPDNPHQYEYMG
                     EWRDMNVIEEIIKVNGGEDITLTVRETIHGPIVSDVMEDEPNALALRWTAANQPSRLL
                     QSVIDINQAQNFDEFREALRLWDIPSQNFVYADVDGNIGYQAPGLIPIRRNGQGLIPV
                     PGWTDEYEWDDYIPFEKLPAMYNPERGYIVTANHAVVDEQYPYFINRDWASGDRGKRI
                     TDMITEIISERPINADDFARIHIDSKSLRAESYVPLLAALSSDNADIQQALDILRAWD
                     LQERTDSVGASIFEIFYMQLAHNILADDVGADNVADVYSTIYFHKLANDPAARWWDDT
                     TTPQTETREDILLKSLEESITWLKGNLGDDMKAWTWGKLHTATFTSTPLGESGIGPIE
                     SIVNRGPFPVDGGSDLVNANSWSWEEPAAVRSHPSMRMIVDMSNFDASRWVIPTGQSG
                     HPFNRNYDDQIKLWLTGEYLSMLSGRDAVEAAATDHLILQPGQ"
     gene            3488..4870
                     /locus_tag="PLC06_07020"
     CDS             3488..4870
                     /locus_tag="PLC06_07020"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRO90233.1"
                     /translation="MYRMPVHYEENDPQFRALCDYVMAEMRRLQVPGVSIGIIHDGRE
                     YLAGFGVTSVENPLPVTADTLFQIGSTTKTLTGTIIMQLVEKGSIDLDEPVKTYLPEL
                     RLKDSYAEGSVTPRHLLTHMGGWVGDFFRETGNGDDALACYVAEMAELEQVVPVGTIW
                     SYNNAGFGLAGRLIEVVTGQTYEEVAQESMLNPLGMTNSYFFPADVISRAFVVGHRVD
                     EEQGVVVERPWALARSAHAVGGLCSSARDQLRYARFQMGDGATEDGTRLLSAGTMTAM
                     QTPQGPANLGRLMGLSWLIKEYGDTRAVLHGGATNGQLSAFLMVPSRGFAITVLTNAD
                     EGSILHDNVVNRALEQYLGIKDPEQPYLELGIEKLSEYVGSYEAALDRLSLYLADGEL
                     MVQMTPKGGFPDVGSPPSPPPPPSRVKFLAPDRAVTIDEPLINMKAEFLRDEGGRINW
                     LRTSRLHRRI"
     assembly_gap    5061..5160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5168..5617)
                     /locus_tag="PLC06_07025"
     CDS             complement(5168..5617)
                     /locus_tag="PLC06_07025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRO90234.1"
                     /translation="MTYTNYPYETNLEVAFRDIDAMGHVNNAVFFAYFETVRIKYLGE
                     LMERSGLLGSELLDLPLILVEANCIYKSPALLTEVLHVGTGISRFGTKSFDMLYRIQG
                     QDGRLVAYGRTVQVMYDYIARSAFPIPDSLRRYIEEYQDGWEPPAYP"
     gene            complement(5626..6756)
                     /gene="galK"
                     /locus_tag="PLC06_07030"
     CDS             complement(5626..6756)
                     /gene="galK"
                     /locus_tag="PLC06_07030"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRO90235.1"
                     /translation="MKQSVINRFEQLFDESPAFVVRAPGRVNLIGEHTDYNDGFVLPM
                     AINRAAWIALRPRRDGLVRIHALNFDDVAEIDLSAMRHEDEGWREYVKGVAYILSEEW
                     NLTGWEGVLAGDVPIGAGLSSSAAVELAAARAFAAVGGLPWDAAHMARVGQRAENEWV
                     GVNCGIMDQMISAAGRRDHALLIDCRSLETQAVPLPTGTVVVVLDTATRRGLVDSAYN
                     ERRAQCEVAAAFFGVPALRDVDMGTFRAGSERLDPVARRRAQHVISENERTLAAAEAM
                     RRGDAAELGQLMNASHVSMRDDFEISREEMDTMVALAQAEPACYGARMTGGGFGGAAV
                     ALVRASDVEAFVASVSDGYRHDTGLEPQIYICTATDGAAVIQ"
     gene            complement(6794..7771)
                     /locus_tag="PLC06_07035"
     CDS             complement(6794..7771)
                     /locus_tag="PLC06_07035"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRO90236.1"
                     /translation="MDPKFPERVSLTFLPTPIEPLERLSRSLGGPEIWIKRDDQTGLA
                     TGGNKARKLEFLVADALAQGADHLVTTGAPQSNHARQTAAAAARFGLGCSLVLRGRRP
                     EITTGNLLLDELFGAHVYWCDTPKCPDEIGRVLNTLRSIGLRPYFVPLGGSNVIGATG
                     YVSAMRELTEQMTAAHLNFDFIVFASSSGGTQAGLVLGARAFGFRGTVLGISVDHPAE
                     SLKTQVAALATATATHIGLDALSVAELVNVNDDYVGEGYARVGETEREAIQMLAQVEG
                     ILLDPVYTGRAFGGLIDLIRWGAFTRGQRVLFWHTGGTAALPAFADRLL"
     gene            complement(7973..9052)
                     /locus_tag="PLC06_07040"
     CDS             complement(7973..9052)
                     /locus_tag="PLC06_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRO90237.1"
                     /translation="MSRTIVILDGDQTGQELLEEALRVLHEDVIRYPLEFKRFDLSLE
                     NRRATKNKVVYEAGKAIREYRVGLKAATITPETRGDVGSPNAILREEMDAEIILRTGR
                     RIPGVRPVGGIYAPISIVRMARDDAYGAREWREGEAGSLDEYAFRTEKISRWVCRAVS
                     EFAFQHARDNRAKVFGGPKFTVSPVYEGMFKEEMDAAAARYPDVRYEPQLIDATFALL
                     LATTGDAMVIPSLNRDGDLLSDMVLQMFGSIAGSESMVISLNQAGVIDCVMAEAPHGT
                     APSLQGKNVANPMAMILAAAGLLGLMKEQELKTASRAIYEATLEAVYDGIRTADLGGS
                     TSTDAFTNEVIRRVKGKLEVWSSLA"
     gene            complement(9104..10222)
                     /locus_tag="PLC06_07045"
     CDS             complement(9104..10222)
                     /locus_tag="PLC06_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRO90238.1"
                     /translation="METYRASTFKLEDSPVTWQATLARLPNAHPLQSWAWGQFKSRWG
                     WEAMPLTMSVGGNSWETIAAAMVLKRRVPRLPYCVLYVPKGPALDFHDRSLRLQVLAR
                     LEQLARRERAIFIKIDPEIVRSWGLEHERVSPLGAAVMRDLQERGWRPSDEQIQFRNT
                     VELSLEATEEQLLAAMKQKTRYNIRLAERKGVTVRPGGPADFPTIVEMYQETAARDGF
                     TVRPAEYYLDGWGGLYQAGIAQPLVAEVDGRAVAAVVLVRFGDRVIYMYGASRNEARE
                     RMPNHLLQWEAIRWAQAQGARVYDFWGAPDEFEETDRMWGVWRFKAGFNGEVVRFIGA
                     WDYTPRPMLYRLYTQAIPRYLELLRARRGRSDEQSLPG"
BASE COUNT         2153 a         3284 c         2977 g         1856 t
ORIGIN      
        1 catcctcgaa cgcgcccagc aacaccgcgc cattatgttc catcgcatgg agcgcatggg
       61 tggggatgat gtccaggtcg ctcatcgtcc aggcgacgcg ctggatttgc tcggcctggg
      121 cgatctcttc aacggtctca agcgctcgaa tcacaatctc cgacatcgaa tcgattttac
      181 ctcacaaacg gctattcaaa agtcgctcgc ccgtgtgaaa gcaactcagt ttatggcaat
      241 caggacgata acggccgcca aagtcataaa cgagccataa ggcagcagat cgccacgtcc
      301 ggcgcggccg gacaatagca gcgcagcggc ggccaccccg gccagcaaga cacccagcag
      361 cagcgccaga ggcagccacg ccagcccgac catcgcgccg atcaagccgg ccagcttgac
      421 gtctcccata cccaatgccc ccggcccata gagacgacgg ccgagcgcat agagagccaa
      481 aaagacgaca aaggccaaca ccgccccgcc cacagcgctc ggcagttcgc cactctgcca
      541 ggcaatgccc agcgcgaaga gcgtggccgg caggacaacc ccattcggca cgcgccgctg
      601 ctccagatcg atcaccgcca acaagatcag gatggcggcg aaggaggcag ccacccacgg
      661 atcgcctgat gccggtgcgc gccaggccaa ccagatgaag aggattggca gcgcgatcag
      721 aaccaccatc tgccgtcgcg gatggcgcgt caggccacat tgcagccgcg cgggaccaag
      781 ccaacaatca gcggcgcgat tgatcagcag agcaacaaac aggcccacca aggcccacca
      841 agcgaaaggc atagagaggg attataacga ccttgcggag ttgcttccat ctttacagat
      901 cgattacttg cgctatcatt tcatcccata ctttgcttgc agatataggg aagaaccatg
      961 aatcgtacca ctcgtatttt attgatcatc ctgggcgttt tggccggtct ggcactggtc
     1021 atggggatcg ctctggcgtc catcacgcgc agccctttcc ccgatattga cggggaaaag
     1081 aaaatggcat tgcaagaagc gtgccggaac gcggccggag agcaaaacgc tatttgcacg
     1141 gcccttgtcg gccatggact gaaaagccca gtccacatct tccgcgacgc caacggtatc
     1201 cccaacatct acgccgagaa cacggaagac ctctacttcg cccagggcta tgtccatgcc
     1261 caggatcgca tgtggcaaat ggagttctgg cgtcatatcg cccaggggcg agtctcggaa
     1321 attgttggcg agccgggcat cgagaacgac aagttcatcc gcaccagcgg ctggaaccgc
     1381 atcacccagg ccaacatcga ttactatgag aaagaacttc ccgaagcaat ggatgcgttg
     1441 aacgcctata gcgccggagt caatgcctat ctggccgaaa aatcaggcga catggccatt
     1501 agccaacgtg tgatggggct ggtgggcgaa ccatgggaga tcgagccgtg gcaacccgcc
     1561 gattcgatcg gctggggagt tgtcatggcc tgggacctgg gcggcaatat gagcgaggag
     1621 cggacgcgcg ccaagttcta cagcctcatc ggcgaagaag ccacagacga actgcttccc
     1681 ggctacccct acgacacacg gcccgtcatc gctcccacgg ccgatctcat ttccgctgca
     1741 accggaggca ataaggcggc ggccgccccg gctatcgact gggcgcgcat cccgaccgac
     1801 atcatcggcg aaccgccggt caccgccttc ggcaccggcc ccgacctggg cagcaacaac
     1861 tgggtcgtca gcggcgaaca tactgaaacg ggcctgccac tgctggccaa cgacccccat
     1921 ctaagcattc aaatgccttc gatctggtat caggttggcc tccacgcgcc gggcatggat
     1981 gtcatcggct tcagcttcgc cggcatcccc ggcgtgatag tgggccacaa caatcgcatc
     2041 gcctggggcg tgaccaacgt cggccccgat gtgcaggacc tctatatcga aaagatcaac
     2101 cccgacaacc cacaccaata cgagtacatg ggcgaatggc gggatatgaa cgtgatcgag
     2161 gagatcatca aggtcaacgg cggcgaggat atcaccctga ccgtgcggga gaccattcac
     2221 gggccaatcg tcagcgatgt gatggaagat gagccgaatg cgctggcttt gcgctggacg
     2281 gccgccaacc aaccgtcgcg ccttctgcaa tcggtgatcg acatcaatca ggcgcagaac
     2341 ttcgacgaat tccgcgaagc gctgcgcctt tgggatatac cgtcgcagaa tttcgtctat
     2401 gccgacgtgg acggcaacat cggttatcag gcacccggcc tcattcccat tcgtcgcaac
     2461 ggccagggct tgatccccgt gcccggctgg acggacgaat acgaatggga cgactatatc
     2521 ccgtttgaga aactgcccgc catgtataac cccgagcgcg gctacatcgt caccgccaat
     2581 cacgccgtgg ttgacgagca atatccctac ttcatcaatc gcgactgggc cagcggcgac
     2641 cgcggcaagc ggatcaccga catgatcacc gagatcatct ccgagcggcc gatcaatgcc
     2701 gacgacttcg cccggattca catcgacagc aagtctctgc gggccgagtc ctatgtcccc
     2761 ttgctggccg cactgtcgag cgataatgcg gatatacagc aggcgctcga tattctgcgc
     2821 gcgtgggatc tgcaggagcg taccgacagc gtgggcgcgt cgatcttcga gatcttctat
     2881 atgcagttgg cccacaacat actggccgac gacgtgggcg ccgacaacgt agccgatgtc
     2941 tacagcacga tttatttcca caaactggcc aatgatccgg ctgcccggtg gtgggacgac
     3001 acgaccacgc cccaaacaga aacccgcgag gacatcctgc tcaagtcgct ggaagaatcc
     3061 atcacctggc tgaaaggcaa tctcggcgac gatatgaaag cctggacatg gggcaagctg
     3121 cacacggcga ctttcaccag cacaccgctg ggcgagagcg gcatcgggcc aatcgagagc
     3181 atcgtcaatc gcggcccctt ccctgtcgat ggcggcagcg acctggttaa cgccaacagc
     3241 tggagttggg aagagccggc ggccgtgcgg tctcatcctt ccatgcggat gatcgtcgat
     3301 atgagcaact tcgacgcctc acgctgggtc atccccaccg ggcagagcgg ccatcccttc
     3361 aaccgtaact acgacgacca gatcaaactg tggctgactg gcgaatacct gtctatgctt
     3421 tcgggccgcg acgcagtcga ggcagcggcc accgatcatc tgattctgca accgggacaa
     3481 taagagtatg taccgcatgc ctgtacacta cgaggaaaac gatcctcaat tccgcgcgct
     3541 gtgcgactat gtcatggccg agatgcgccg cctgcaagtg cccggcgtct ccatcggtat
     3601 tatccacgac ggccgcgagt atctggccgg atttggcgtg accagcgttg agaatccgct
     3661 acccgtgacg gccgacaccc tgttccagat tggctcgacc accaagacgc tgaccggcac
     3721 gatcatcatg cagctggtag agaaggggtc gatcgatctg gacgagccgg taaaaacgta
     3781 cctgccggag ttgcgcctga aggacagtta cgccgaaggt tcggtaacgc cgcgccatct
     3841 gctgacccac atgggcggct gggtaggcga tttcttccgc gaaacaggca acggcgacga
     3901 cgcgctggcc tgctacgtgg ccgaaatggc tgaactggag caagttgtgc cggtgggcac
     3961 aatatggtcc tacaacaacg ctgggttcgg gctggccggg cgtctcatcg aggtcgtcac
     4021 cggtcaaacc tatgaagagg tggcccagga gtcaatgctc aatcccctgg gcatgaccaa
     4081 ctcctacttc ttccccgccg acgtcatctc ccgcgccttc gtggttggtc atcgagtgga
     4141 tgaggagcag ggcgtcgtcg tagagcggcc gtgggcactg gcgcgttcgg cccatgccgt
     4201 gggcggcttg tgctccagcg cgcgtgacca gttgcgctat gcccgtttcc agatgggcga
     4261 cggcgcgacc gaggacggca cgcggctgtt atcggccggg acgatgaccg ccatgcagac
     4321 cccgcaaggc cccgccaatc tggggcgatt aatgggcctg agctggctca ttaaagagta
     4381 cggcgacacg cgcgctgtgc ttcatggcgg cgcgactaac ggccaactct cggcgttcct
     4441 gatggtgccg tcgcgcggct tcgccatcac cgtgttgacc aatgccgatg agggcagcat
     4501 tctccacgat aacgtcgtca accgggcgct ggagcagtac ctgggcatca aggatccgga
     4561 gcaaccctac ctggaattgg ggatcgagaa gttgagcgaa tatgtcggca gttatgaggc
     4621 ggcgctggat cggctgtctc tctatttggc cgacggggag ctgatggtcc agatgacgcc
     4681 gaaaggcggg ttcccggacg taggctcgcc tccctcgccg ccgccaccgc cgagccgagt
     4741 gaagtttctc gcacccgacc gggccgtcac catcgatgag ccgctgatca acatgaaagc
     4801 ggagttcctt cgcgacgaag gcggccgcat caattggttg cgcaccagcc ggctccatcg
     4861 ccggatatag cgaaaaggcc ttacatgaaa cagggcagcg tgaccgtcca cgctgccctg
     4921 ttgactttct cattgacgac gcgaatcctg tcgcgcgacc actggcccac tgatcactga
     4981 cctactggcc actgacctac taatcactgt ctctagggat acgccggcgg ctcccaccca
     5041 tcctggtact cctcgatgta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 actgtctcta gggatacgcc ggcggctccc acccatcctg gtactcctcg atgtaccgac
     5221 gcaagctgtc ggggatggga aaggcgctgc gggcaatata atcatacatc acctgcaccg
     5281 tgcggccata ggcgacgagg cggccgtcct gcccctgaat gcggtagagc atgtcgaaac
     5341 tcttcgtccc gaagcgggaa atgcccgtac ccacgtgcag cacctcagtt agaagtgccg
     5401 gcgacttgta gatgcagttc gcctcgacga gaataagcgg caaatccagc agttcggatc
     5461 ccaaaaggcc gcttctctcc atcagttcgc ccagatactt gatacgtacc gtctcgaaat
     5521 aggcgaagaa gacggcgtta ttcacatggc ccatcgcgtc aatatcgcga aaggcgactt
     5581 ccagattggt ttcgtaaggg taattggtgt aggtcatagg ttcattcatt gtatgacggc
     5641 cgcgccgtcg gttgccgtgc agatatagat ttgtggctcc aggccggtat cgtgccgata
     5701 gccatcgctg accgaggcga cgaatgcctc gacgtcggat gcgcgcacga gggccacggc
     5761 cgcgccaccg aatccgcccc cggtcatcct ggctccgtag caggctggtt ctgcttgagc
     5821 cagcgcgacc atagtatcca tttcctcgcg ggagatctcg aagtcgtcgc gcatgctgac
     5881 atggctggca ttcatcagct ggcccaactc ggccgcatcg ccacggcgca tcgcttcggc
     5941 cgcggccagc gtgcgctcgt tctcgctgat cacgtgttgc gcgcgccgtc gtgccaccgg
     6001 gtcgagtcgt tcgctgccgg cacgaaaggt tcccatatcc acatcgcgca gcgcggggac
     6061 gccgaaaaag gcggcggcca cttcgcattg cgcccggcgc tcgttgtagg ccgaatcgac
     6121 caatccgcgc cgcgtggccg tatcgagcac aaccacaacc gtgcccgtgg gcaaggggac
     6181 ggcctgcgtc tccagactac ggcagtcgat cagcaaggcg tgatcgcgtc gtccggcggc
     6241 ggagatcatc tgatccatga tgccgcaatt cacgcccacc cattcgttct ctgcgcgctg
     6301 gccaacgcga gccatgtgag ccgcatccca gggcaaaccg ccgacggcag caaaggcgcg
     6361 cgccgcggcc agttccacgg cggccgacga tgacaacccc gcgccgatgg gcacgtcgcc
     6421 ggccagtacg ccttcccagc cggtcaggtt ccactcttca ctgaggatat aggccacgcc
     6481 cttcacatat tcgcgccaac cttcatcctc gtggcgcatg gcgctcagat cgatctcggc
     6541 gacgtcgtcg aaattgaggg cgtggatccg aacaaggcca tcgcgacgcg gccgcaaggc
     6601 aatccaggcg gcgcgattga tggccatcgg cagaacgaag ccatcgttgt agtcggtatg
     6661 ctcgccgatg aggttaaccc gtcctggggc gcgaacgacg aaagcgggtg actcgtcgaa
     6721 aagctgctca aaccggttga tgaccgactg tttcataggg gtcgtacctg gaatggaccg
     6781 cactgtggtc aagctagagc aggcgatcgg caaacgcggg cagcgcggcc gtaccgccgg
     6841 tatgccaaaa caatacccgc tggccccgcg tgaaagcgcc ccagcgaatc agatcgatca
     6901 atccgccaaa agcccggcct gtatacaccg gatcgagcaa aataccttcc acctgagcca
     6961 gcatctggat cgcctctcgc tcggtttcgc caacgcgggc atagccttca ccgacgtagt
     7021 cgtcgttgac gttaaccagt tcggcgacgg atagggcatc caatccaata tgggtagctg
     7081 tagccgtagc cagggccgcc acctgtgttt tgagcgattc ggcggggtga tcgacgctga
     7141 tgcccagcac cgtcccacgg aagccgaagg cccgcgcgcc cagcaccagt ccggcttgcg
     7201 tgccgccgct gctgctggcg aagacgataa aatcgaaatt cagatgggcg gccgtcatct
     7261 gttccgtcag ctcgcgcatc gccgacacat agcctgtcgc gccgatgaca ttggaaccac
     7321 cgagaggcac aaagtagggg cgcagaccga tcgaacgcaa tgtgttcagg acgcgtccaa
     7381 tctcgtcggg gcactttggc gtgtcacacc agtacacatg cgccccgaac agctcatcga
     7441 gcaggagatt gccggtcgta atttccggac gccggccgcg caacaccagg ctgcaaccga
     7501 ggccgaaacg ggcggcggcg gcggccgtct gccgggcatg attgctctgg ggagcgcccg
     7561 tagtcaccag atgatcggct ccctgagcca gcgcgtcggc caccaggaac tccagcttgc
     7621 gcgctttgtt gcccccggta gccagcccgg tctggtcgtc ccgcttaatc cagatctccg
     7681 gcccgcccag gctgcggctc agtcgttcca gcggttctat cggggtcggc aagaaagtaa
     7741 gcgagactct ctcaggaaat ttcggatcca taaataattc cagttgggtc gaggttcaaa
     7801 cccccacgcc atttgccgat attgtagcct aattggtcgg gctgtcgatt gcccctatat
     7861 cagtcgcggc gtgggtttcg aagggttagg cgcgccgcga gtgcgctttc tttgcccgcc
     7921 ggaaattaca ttgatcacgc tggaacccca tcgatagata tggtttcgat ccctacgcca
     7981 gtgacgacca gacctccagc ttacccttca cgcggcggat gacctcgttg gtgaacgcgt
     8041 cggtagaggt gctgccacca agatcggccg tgcgaatgcc atcataaacc gcttccagcg
     8101 tcgcctcata gatggcccga ctggccgttt tcagctcctg ttccttcatg aggcccaata
     8161 acccggcagc agccagaatc atggccatcg gattcgccac gttcttgccc tgcaagctgg
     8221 gggccgtgcc atggggggcc tcggccatga cgcaatcgat cactccggcc tgattgagcg
     8281 agatgaccat cgattccgaa ccggcaattg agccgaacat ctgcaagacc atgtcggaca
     8341 gcagatcgcc gtcgcggttc aacgatggga tgaccatcgc gtcgccggtc gtggccagca
     8401 gcagggcgaa ggtggcgtcg attagctgtg gctcatagcg cacatcggga tagcgagccg
     8461 cggccgcatc catctcttcc ttgaacatgc cctcatagac ggggctgacg gtgaacttgg
     8521 ggccgccaaa caccttggcg cggttgtctc gggcgtgctg aaaggcaaac tccgacaccg
     8581 cgcggcacac ccagcgcgag atcttctcgg tgcgaaaggc gtattcatcg aggctgcccg
     8641 cctccccttc gcgccattcg cgcgcgccat aggcgtcatc gcgagccatg cgcacgatac
     8701 tgatcggcgc gtagatgccg ccaaccggcc gcacaccggg gatacggcgc cccgtgcgca
     8761 agatgatctc cgcgtccatc tcctcgcgca ggatggcgtt ggggctgccc acgtcgccgc
     8821 gggtctccgg agtgatggta gctgctttca gaccaacccg atactcgcga atcgccttgc
     8881 ccgcctcgta aacgaccttg tttttcgtgg cccggcggtt ctccaggctg aggtcgaatc
     8941 gcttgaattc cagggggtag cggatgacgt cctcatgcag gacgcgcaac gcctcttcca
     9001 gcaactcctg cccggtctga tccccatcga gaatgacaat tgtcctgctc atcttcttcc
     9061 acacctctct aatctgatta cacctatccg gcagagccga tagttagccg ggcaacgact
     9121 gctcatcgga tcggccgcgc cgggcgcgca atagttccag gtaacgcggg atggcctgcg
     9181 tgtacaggcg gtagagcatc gggcgcggcg tataatccca cgcgccaata aaacgcacga
     9241 cctcgccatt gaatccggcc ttaaaccgcc agacccccca catgcgatcc gtctcttcaa
     9301 attcatcagg cgcgccccag aaatcataga ccctggcgcc ctgcgcctgc gcccagcgaa
     9361 tcgcctccca ttgcaggagg tggttgggca tccgctcgcg ggcttcgttt cgcgatgcgc
     9421 cgtacatata gatgactcgg tcgccaaaac gcaccaaaac cacggccgcc acggcccgcc
     9481 cgtcaacctc ggccaccagc ggttgggcta tgccggcctg ataaagcccg ccccaaccgt
     9541 cgaggtaata ttccgccggt cggacggtga acccgtcgcg cgcggccgtt tcctgataca
     9601 tctcgacgat tgtcggaaaa tcagccggcc cgccggggcg aacggttacg cccttgcgct
     9661 cggccaggcg aatattgtaa cgagtcttct gcttcatggc cgccaatagt tgttcttcgg
     9721 tggcttccag cgatagctcg accgtattgc ggaactgaat ctgctcatcc gatggccgcc
     9781 agccccgctc ctgcagatca cgcataacgg ccgcccccag gggcgagaca cgctcatgct
     9841 ccagccccca gctacgcacg atctcgggat cgatcttgat gaagatcgcc cgctcgcgcc
     9901 tggccagttg ttccaggcgc gccaatactt gcaatcgcaa cgagcgatcg tggaaatcca
     9961 gcgccggccc cttgggcacg tagaggacac aataaggcag tcggggcaca cgccgcttca
    10021 gtaccatcgc cgcggcgatc gtttcccagc tattgccgcc taccgacatc gtcagcggca
    10081 tcgcctccca gccccagcgc gatttgaact gtccccaggc ccaactctgc aagggatgag
    10141 cgttaggcag gcgcgccaaa gttgcctgcc aggtgacagg cgaatcctcg agtttgaacg
    10201 tcgaagcgcg gtaggtttcc attgccgtag acatcgttac aaaaagggcg gcgccgggcc
    10261 gcgatccgct gaataatact ccgaacgagc ggagggtgca cccatcgttc gaactcggca
    10321 gcaattcgaa atatgaaagc cgtcagacta tactggctgc atgagagcga
//
LOCUS       DAOMQG010000093        10340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2779_length_10340_cov_13.453865, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000093 DAOMQG010000000
VERSION     DAOMQG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2779_length_10340_cov_13.453865"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..699)
                     /locus_tag="PLC06_07050"
     CDS             complement(196..699)
                     /locus_tag="PLC06_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90239.1"
                     /translation="MKSKEFFAACADAFSPLMTQFGLEAAGQHDRKDVWEWVLKNRTT
                     GIRVIYELKDYYIGFQICRLENGRLKPFMGEMRPDSALSCFDLLDLLSIRIPNEKHEP
                     IEEFKLSKYSLPEILSRSAEYLYNLAGDVLEGNFEVFDSLDRIVKDRARVAAFKKWGD
                     KAHEFGW"
     gene            complement(918..1451)
                     /locus_tag="PLC06_07055"
     CDS             complement(918..1451)
                     /locus_tag="PLC06_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRO90240.1"
                     /translation="MNARYYLPEIGRFISLDTIVPEPGNPQSYNRYSYSLNNPVKYND
                     PSGHWFESVLDLGFIAYDIYDIQQNGLTWTSGLSLAADMAGLALPVVTGGGAAVRALA
                     HADAVTNVVTHIDEAIDAGWAIESLVEIGHTIDNLLGATKAFEETGRVQLVPQKRVVI
                     GEGMDAVKAAGREHGAK"
     gene            complement(1363..1827)
                     /locus_tag="PLC06_07060"
     CDS             complement(1363..1827)
                     /locus_tag="PLC06_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90241.1"
                     /translation="MTTQPDGTIIYTPFPDYELTDPPTGADTVRTTYRLGGQIVAVQT
                     KVGAAAGVFYYTYTDHLGNITALSWTGGTHVPNSNARYDPFGTMTTAPATNPSISNHG
                     FTGHKRCPHRPQQHRQPRHRLDLHERPVLSAGDWAVYFAGHDCAGAGEPAKL"
     gene            1896..2084
                     /locus_tag="PLC06_07065"
     CDS             1896..2084
                     /locus_tag="PLC06_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90242.1"
                     /translation="MACAFYALAYKVGVRNLPEGIGARQFVRDQLDRLGLGHELTHIP
                     WGSKRYRLPPSRVSEVGD"
     gene            complement(2151..2522)
                     /locus_tag="PLC06_07070"
     CDS             complement(2151..2522)
                     /locus_tag="PLC06_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90243.1"
                     /translation="MNQFQSTEDIRIFCGFLIQLATDIGDEKARLLLQEALAAGNTSS
                     PTERLGETIEGLLVVREIMLRDDRYSVDVIYEVNEAIKTGQKMLRRANRPWFFWLFLI
                     SLRLFPSLYVSFIFQMQVGKQ"
     gene            3370..3939
                     /locus_tag="PLC06_07075"
     CDS             3370..3939
                     /locus_tag="PLC06_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRO90244.1"
                     /translation="MSATTASNSNAWIWTVIKGILALGLGLFLLFSAATAPVWIAYAL
                     AIYMAASGGMQTFSGLLKRDAVGSKTDRIRGLVGLLGGLALLLLAYFNVLSLGAGYTL
                     LAILLIVFGGLGLFEALFDRGAERFRWMPLLVNLLLIGLGIMVFYSRIREFDLRLWSG
                     LVLAIIGLIIIVYGHLFQRTNPKANAADV"
     gene            4132..4911
                     /locus_tag="PLC06_07080"
     CDS             4132..4911
                     /locus_tag="PLC06_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90245.1"
                     /translation="MSQLITPILNVNSVSLSFGGNAALSEVSFNVLRGEIRAIIGPNG
                     AGKTSILNCISGFYRPQQGNILFKGDTDLTRTRTDQIARLGLARTFQNIALYTGLSTL
                     DNLMAARHIHMKQNALQSILYWGPARREEVRHREVVEDIIDFLEIAHIRKAVVGALPY
                     GLRKRVELGRALAMEPELLLLDEPMAGMNLEEKEDMARFILDIYERRGVTIVLIEHDM
                     GLVMDISDRVVVLDFGRKIADGTPDEIKRNPAVIQAYLGEA"
     gene            5001..6917
                     /locus_tag="PLC06_07085"
     CDS             5001..6917
                     /locus_tag="PLC06_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRO90246.1"
                     /translation="METSAQTVPQFFMHQVQTQPPDKVAVRQKELGIWREFTWVDSYE
                     QVRDFAMGLIALGVQRGDKICTVGDNDRQYLWAYLGLQAAGAAQVGLFTDATPAEMAY
                     VIEHSDATFILAKDQEQCDKMLEIRDRIPNVRKVIYWDDQGLWNYDDPWLVPFEAVQQ
                     LGRGLVEREPGRFDTEVALGGVDDIAMLCYTSGTTGLPKGVMLSHGNIMATIKLYDGV
                     DPRRDTDNHVSFLPLGWIAEPILGFAAHVFAGVIINFPEAPETVRQNIREIAPDMLFY
                     NARLWDSLVGMVQVRMNDATRINRALYRRFLPVGYRMADAKFSGQEPGIGLRAAYAAG
                     DRLVFAPLRDQLGLSRIRAAYTAGSSLSPDAMRFFHALGINLKQIYGSTEVSGGATVH
                     RDNAIKFASVGQPAPGVELRIAPDGEIQITGPMVMQGYYKDPANTAKDILEESDGRRW
                     FRSGDAGYIDDDGHLIYLDRVKDMLTLANGERFSPQFIEGRLKFSPYVRDVMAVGGEN
                     RAFVTALVIIDFENVGQWAEKQRIGYTTFVDLSQKPQVYELVRRTMEEVNESLPAAAR
                     VQRFVLMHKEFDADEAEMTRTRKLKRNVLLDRYGAIVEGMYDGAEMIQVRAPVRYQDG
                     SEGFVETEIRVMSL"
     gene            6950..7645
                     /locus_tag="PLC06_07090"
     CDS             6950..7645
                     /locus_tag="PLC06_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HRO90247.1"
                     /translation="MIHFYIDESGTGLRDRRSPYFVLAATAIPVSELEGVDYRINTFK
                     RKLIPFAEPEDFEFKVRELRRGDRFFQGISWPVRAEAIVEMSSIVAELPCEIVAVQVD
                     KRNLTEYIATDGQLYALALRKLVEMVEKVLEQRMEPGMLMVDSRSDLHSSVQDRRLLD
                     VYRHIQARKHSRIVGLPWFGFSAFYVGLQIADFAAYMIDFVSNEVDTPFGSAALQAAF
                     RHIENKVRLARIP"
     gene            7810..8745
                     /locus_tag="PLC06_07095"
     CDS             7810..8745
                     /locus_tag="PLC06_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO90248.1"
                     /translation="MNWQLVPQQLATGLLNGGIIAIIALGIVLIYRSSEVFNFSHGQL
                     VMLGAFLTWWFAGGTENGKEIFNLPLWGAVPLAIIVMAGLGLLIERLALRPMTGQPLL
                     AIILMTLGLAQLLDGTAAIIFGTEPKNNFPAPFSPSDVIKIPFPGAFMDVIILKQALL
                     ATAIVAVIAAVIFMIFFQYTRIGLAMRATSEDHELARGVGINVPRVFGLSWAIAGIIA
                     TVGGVLLATVTGVSLSLATITLVAFPAILLGGLESFSGAIIGGLLVGLAQALVQASSV
                     QTIRGSSDIAPYILLLIVLVIRPEGLFGEKRIERV"
     gene            8749..9816
                     /locus_tag="PLC06_07100"
     CDS             8749..9816
                     /locus_tag="PLC06_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO90249.1"
                     /translation="MAALRPAGDFDRTYEQDMGVIRRPWQWLALGVGFLLLLTAPRWA
                     GAYLVSTANRIFILVIAVQGLNILVGYTGQISLGQAAFMLVGGYTSALVTSHLSVPFP
                     LALLIAGLVSGLIGLLFGLPSLRVKGFYLAMATLAAQFIIPWVSRHLYPDLLGGPAGR
                     ISADVPVIFGISFGEVGNYYYLSLITLVITTVAVLNISRSRLGRAFVSLRDNDLAAEL
                     LGVNLFDYKLRAFFIASFLAGVAGALRAHSQRGVGTELGYGLAESIIMLGMLVIGGLG
                     TNLGPFLGVTMVVLLEDTAGVAGSTMAKLFPTQAARFLTSFRPIFFGLVLMLFLIFEP
                     RGLAHRWQTFKAAWRLRPFAR"
     gene            9937..>10340
                     /locus_tag="PLC06_07105"
     CDS             9937..>10340
                     /locus_tag="PLC06_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90250.1"
                     /translation="MKKISLVSLLLVMAIMLAACGGAAPTVEQLAPTVQSVVEQVAPT
                     IAAVATEVAPTVAAVATAVAPTEVPPTEVPPTEEPAAPEVISGESTNDDCAAMYDGES
                     IHVHQAAGLTGPLAQILGDGFINGSKAAVDRI"
BASE COUNT         2089 a         3049 c         2884 g         2318 t
ORIGIN      
        1 cttcgtctgc acggccacaa tctgccccgc cagcctgtag gtcgtgcgaa cggtatccgc
       61 tccgctgctc cgtggtccag tactcgattg tttaagcgca gttgccgaac tgacccgtct
      121 ggtatgacaa cttcacttcg gctaatatgt cgatttctct tatcttatgt cagaaaagaa
      181 gatttatcat atagattacc atccaaactc atgcgcctta tccccccatt ttttgaaggc
      241 ggctactcgg gccctgtctt tcacaatcct gtccagagaa tcgaacacct caaaattccc
      301 ttcaagcaca tccccggcaa ggttatacaa atattcagcg gaacgactga ggatttccgg
      361 caatgagtat ttcgatagct tgaactcttc aataggctca tgcttttcat tgggaatgcg
      421 tattgatagt agatctaata ggtcaaagca ggatagggca gagtctggcc tcatctctcc
      481 catgaagggc ttgagtcgac cattttccaa cctacagatt tgaaagccta tatagtaatc
      541 cttcaattca taaatgaccc tgattcccgt cgtgcgattt ttcagcaccc actcccatac
      601 atctttcctg tcgtgttgac cagcagcctc caggccgaat tgtgtcatta gtggcgagaa
      661 ggcatcagca caagctgcaa agaactcctt tgatttcatt ggggtctcgc aagcggggtc
      721 gttggatagt tcaagtcgct taaaatcccc ttttctagtt cgtagaatat actgcgaact
      781 ggacgatttg gatcgatgcc tatgtccata atttcgtaac cttccctaac ttttccttga
      841 atccaacgtt cctggcgcgc aagagcctct gcctcattat aaatttccgg cttaaagttc
      901 tttccccaag cttgatatca cttagcaccg tgttcgcgtc cagcggcttt gaccgcatcc
      961 ataccttcac caattacgac tctcttttga ggaacgagtt gaactcggcc agtctcttcg
     1021 aaagcttttg ttgcacccaa tagattatcg atagtgtggc caatttcaac aagggactct
     1081 attgcccacc ccgcatctat cgcctcatca atatgcgtca cgacattagt gacggcatcc
     1141 gcatgcgcca gcgcccgaac ggccgccccg ccgcctgtca ctaccggcag tgccagcccg
     1201 gccatatcag cggccaatga tagcccgctc gtccaggtca gcccattttg ctggatgtcg
     1261 tagatatcgt aagcaatgaa tcccagatcg agaaccgatt caaaccagtg acctgagggg
     1321 tcattatact tgacagggtt attgagcgag tagctgtacc ggttatagct ttgcgggttc
     1381 cccggctccg gcacaatcgt gtccagcgaa ataaaccgcc caatctccgg cagatagtac
     1441 cgggcgttca tgtagatcaa gtctatgtcg tggctgccgg tgttgttgtg ggcgatgagg
     1501 acatcgcttg tggccggtga agccgtggtt ggagatggac gggttggtgg ccggagccgt
     1561 cgtcatcgtt ccgaaggggt cgtagcgggc gttggagttg ggtacatgtg tgccgcccgt
     1621 ccaactgagc gccgtgatat tccccagatg gtcggtgtaa gtatagtaga acacccccgc
     1681 cgccgcgccc accttcgtct gcacggccac gatttgtccg ccgaggcggt aggtcgtgcg
     1741 aacggtatcc gctcccgtcg gcgggtcggt cagttcgtag tcggggaagg gggtgtagat
     1801 gatcgtcccg tcgggctgcg tggtcatcac ccgttggccg tcggcgtcgt atttggttgt
     1861 tcaggtgcaa aattcggttc gccacggatg agcaggtggc ttgcgcgttc tacgccctgg
     1921 cctacaaggt cggcgtcagg aacctgcccg aaggcatcgg cgcgcggcag tttgtccgcg
     1981 accaactcga ccgactgggg ctgggccatg aactgacgca catcccctgg ggcagcaagc
     2041 ggtatagatt gccaccgtcg agagtgagtg aagtgggcga ttagttatac gttgcggttg
     2101 taaaggtaca ggctccagtt acggcgtgaa tgctagccgg gcttcgaagg ctattgtttg
     2161 cctacttgca tttggaaaat gaacgagaca tatagtgaag gaaacaaccg gagcgagatg
     2221 aggaaaagcc aaaaaaacca aggcctgtta gcccttctta acattttttg gcccgttttt
     2281 atggcttcat tgacttcata aatcacgtca acggaatatc tgtcatccct taacattatc
     2341 tcgcgcacca ctaacaatcc ttcaatggtc tcgcccagcc tctctgttgg agaggaagta
     2401 ttgcctgctg ctagagcttc ctgaagtaaa aggcgggctt tttcatcacc aatatccgtg
     2461 gccaactgta tcaaaaagcc acaaaagatt cggatatctt ctgtggattg aaactggttc
     2521 attcttttca ccctttcaat attattgcat atggaagtag tgtgcctgaa ctccaccggg
     2581 ctgagtgaca atttctagcg cacctccaaa gttttggcca acacctggtg ctggccgagt
     2641 gataaaagaa gtgttcgatc tgacttgccc acctataata aagtccatat tagcaacgct
     2701 gctggatcca gggaggccat attgttgagc ataacccgga gttaacgggc tttgaggagc
     2761 ccagaactgg ccctcaaccg cagcactatg gccgagattt cccgcacggt atacctgctg
     2821 acccttcgcg acttcagcac gcaacttcag tgcactgggc cgcagactct gatatgcatg
     2881 tccataatcc gtctcgaccg atagcttcga gaaaagagaa gttgctgcct tggacacgcc
     2941 gtaccctgtc ccggctaaag ccccaccaat ggttgcatca attgcaatgt cctgtagttc
     3001 tcccactcct tgaagcagag ggcgatctga cagagcattt gttgcaatgc gaccaccttg
     3061 tccagcgagc gccccactaa ctgcaccggt gcctacagtc gccgcaaaac ctgtaccaat
     3121 aatcgctgtt ccaataccaa acgtcgctcc tcgacaggcc ccccatcctc aataccattc
     3181 agaagtagaa tattctatca ataaccggcc tcggccgcat accggcgaga gcgacgaacc
     3241 ccgtctccca ggcatccgcg atgccggcaa acacccgttc ataggcacaa ccctcaattg
     3301 gtgcataccc acccggatca gtataatcag tcaggtcgtt atgttataga aacaggagag
     3361 ttattatcaa tgagcgcaac aactgcatca aattcaaatg cctggatctg gacggtcatc
     3421 aagggcatcc tggccctggg attggggttg ttcctgctgt ttagcgcggc gaccgcgccg
     3481 gtctggatcg cctacgcgct ggccatctac atggccgcgt cgggcgggat gcagaccttt
     3541 tccggccttc tgaagcggga cgcggtcggc tcgaaaacag accggattcg cgggctggtg
     3601 gggctgttgg gtggcctggc gctgctgctg ctggcctatt tcaacgtgct ctcgctgggc
     3661 gccggctaca cgctgctggc cattctgctc atcgtcttcg gcgggctggg gctgttcgag
     3721 gcgcttttcg atcggggcgc ggagcgcttc cgctggatgc cgctcctcgt caacctgctc
     3781 ctgatcggac tgggcatcat ggtcttctat tcgcgcatcc gggaattcga cctgcggctg
     3841 tggagcgggc tggtgctggc catcatcggg ctgatcatca tcgtctacgg ccaccttttc
     3901 cagcggacga accccaaggc aaatgcggcc gacgtataaa gcaaacacct gagaaccctg
     3961 acgggcgggc gacacaaggg agctatcacg acaccaaaac ggatcgtcgg ccgcctgtca
     4021 ggattgccct ttgttttaaa caactgaaaa cgctgcaatt ccgcgctgtt gtttgcgcct
     4081 cgcggccaca tatgatacct tttgccgcta cgtggacaca ctgacgcgcc catgagccaa
     4141 ttaataacac ctattctcaa tgtcaatagc gtttcgctct cttttggggg aaacgccgca
     4201 ctttctgaag tctccttcaa tgtcctgagg ggggaaattc gcgcgattat cgggcctaat
     4261 ggcgcgggca agacttctat cctcaactgc atctccggct tctatcgtcc ccaacaggga
     4321 aacatcctat tcaagggcga caccgacctg acccggacgc ggaccgatca gatcgcccgc
     4381 ctcggcctgg cgcgcacgtt tcagaatatc gccctctaca ccggcctcag cacgctcgac
     4441 aacctgatgg ccgcccgcca catccacatg aagcaaaacg cgctgcagag catcctctat
     4501 tgggggccgg cccggcgcga ggaagttcgc catcgcgagg tggtcgagga catcatcgac
     4561 tttctggaga tcgctcacat ccgcaaggcg gttgtcgggg cgctgcccta cggtctgcgc
     4621 aagcgggtgg agctgggccg cgcgctggcc atggagccgg agctgctgct gctcgacgag
     4681 ccgatggccg gcatgaacct ggaagaaaaa gaggacatgg cccgcttcat cctcgatatc
     4741 tacgagcggc gcggcgtgac catcgtcctc atcgagcacg acatggggct ggtgatggac
     4801 atctccgatc gcgtggtggt gctcgatttc ggccgcaaga tcgccgacgg cacgcccgac
     4861 gagatcaagc gcaacccggc cgtcatccag gcctatttgg gagaagcctg atccaattac
     4921 gaattacgaa ttacgaatta cgaatgaaga acactccttc tgaattcctg gttcctaatt
     4981 cctaatttct aattcaagtt atggaaacgt ccgcacagac agtaccgcag tttttcatgc
     5041 accaggtgca gacccagccg ccggacaaag tcgccgtgcg gcaaaaggag cttggcatct
     5101 ggcgagagtt cacctgggtc gactcctacg agcaggtgcg cgatttcgcc atgggcctca
     5161 tcgccctggg cgtgcagcgc ggtgacaaaa tctgcaccgt gggcgacaac gaccggcaat
     5221 acctgtgggc ctacctcggt ttgcaagccg ccggtgcggc ccaggtaggc ctgttcaccg
     5281 acgccacccc cgccgaaatg gcctacgtca tcgaacacag cgacgccacc tttatcctgg
     5341 ccaaggatca ggagcaatgc gacaagatgc tggagattcg cgaccggatc cccaacgtcc
     5401 gcaaagtcat ctattgggat gaccaggggc tgtggaatta tgatgatccg tggctggtgc
     5461 ccttcgaagc cgtgcagcag ctcggccgtg ggctggtcga gcgcgaaccg ggccgcttcg
     5521 atactgaagt cgctctcggc ggggtcgatg acatcgccat gctctgctac acgtcgggca
     5581 cgaccggatt gcccaagggc gtcatgctca gccatggcaa catcatggcc accatcaagc
     5641 tctatgacgg cgtcgacccg cgccgcgaca ccgacaacca cgtcagcttc ctgccgctgg
     5701 gctggatcgc cgagccgatt ttgggcttcg ccgcccacgt cttcgccggg gtgatcatca
     5761 atttccccga agcgccggag acggtgcggc agaacatccg cgagatcgcg ccggatatgt
     5821 tgttctacaa cgcccggctg tgggactcgc tggtcggcat ggtgcaggtg cgcatgaacg
     5881 acgcgacgcg gatcaaccgg gcgctctacc ggcgcttcct gcccgtcggc tatcgtatgg
     5941 ccgacgccaa attcagcggc caggagccgg ggataggcct gcgcgccgcc tacgccgccg
     6001 gcgatcggct ggtgttcgcc cccctgcgcg accagctcgg cctgtcgcgc attcgcgccg
     6061 cctacaccgc cggttcgtcg cttagccccg acgccatgcg cttcttccac gcgctgggca
     6121 tcaacctgaa gcagatctac ggctccaccg aggtctccgg cggggcgacc gtccatcgcg
     6181 acaacgcgat caagttcgcc agcgtcggcc aacctgcgcc gggcgtcgag ttgcgcatcg
     6241 cccccgatgg cgagattcag atcaccgggc cgatggtcat gcagggctac tacaaagacc
     6301 cggccaacac cgccaaggac atcctggagg aatccgacgg tcgccgctgg ttccgctccg
     6361 gcgatgccgg ctatatcgac gacgacggcc atttgatcta cctcgaccgg gtcaaggaca
     6421 tgctgacgct ggccaacggc gaacgcttct cgccgcagtt catcgaggga cggctcaagt
     6481 tcagccccta cgtccgcgac gtgatggctg tgggcggcga gaaccgcgcc ttcgtgacgg
     6541 cgctggtcat catcgacttc gagaatgtcg gccagtgggc cgaaaagcag cgcatcggct
     6601 acacaacatt tgtcgacctg tcgcagaagc cgcaggtgta tgaactggtg cgccgcacga
     6661 tggaagaggt gaacgaatcg cttccggccg cggcgcgtgt gcagcgcttc gtgctgatgc
     6721 acaaggagtt cgacgccgac gaagcggaga tgacccgcac gcgcaagctg aagcgcaacg
     6781 tcctgctcga tcgttacggg gccatcgtcg aagggatgta cgacggcgcg gaaatgattc
     6841 aggtgcgcgc cccggtgcgc tatcaggatg gcagcgaggg gttcgtcgaa acggagatac
     6901 gggtgatgag cctatgagac tattggctac cgctcaataa attcatgcca tgatccattt
     6961 ttacatagac gagagtggaa caggtctacg cgatagacgt tcaccttact ttgtattagc
     7021 ggctacagca atccctgtga gcgagttgga aggcgttgat tatcgaataa ataccttcaa
     7081 acgaaaactg atcccttttg cggagcccga ggattttgag tttaaggtgc gagaattacg
     7141 gcgtggtgat cgcttttttc aaggaatttc gtggcccgta cgcgccgaag cgatcgtcga
     7201 gatgtcgtca attgtggctg aattaccatg cgagatcgtt gctgtacaag ttgacaagcg
     7261 taatttaaca gagtatatcg ctacggatgg ccaactatac gcgcttgccc ttaggaaact
     7321 tgtcgaaatg gtggaaaaag tattggagca aagaatggaa ccggggatgt tgatggtcga
     7381 ttcacgctct gatttacatt caagtgtaca ggaccgcagg ctgctggatg tctaccggca
     7441 tattcaagcg cgaaaacatt ctcgtattgt tggtttaccc tggttcgggt tctcggcttt
     7501 ttacgttggt ttgcagatag cggattttgc ggcctatatg atagattttg tatcgaatga
     7561 agttgataca ccttttggaa gcgcggcttt gcaggcagct tttcgccaca ttgaaaacaa
     7621 agtgaggtta gcccgaatac cataaaaaca aacccacgat catatcgatc gtgggttctt
     7681 ggttacgcct gataggcgca cagcatcaca gccatgctat tgatccccgt gcagtccggg
     7741 gcacctcatc aacaaggatg agtatataac agatcggagt cttaggtcaa tgcagattat
     7801 agagcgttca tgaactggca actcgtccct caacaactcg ctaccggcct gctcaacggc
     7861 ggcatcatcg ccatcatcgc tttgggcatc gtcctcatct accgctcgtc ggaggtgttc
     7921 aacttctccc atggccagct cgtcatgctg ggggccttcc tgacctggtg gttcgccggc
     7981 ggaaccgaga acggcaagga gatttttaat ctgccgctgt ggggggctgt cccgctggcc
     8041 atcatcgtca tggccgggct gggcttgctc atcgagcgtc tggccttgcg gccgatgacc
     8101 gggcagccat tgctggcgat catcctgatg acgctggggc tggcgcaatt actggacggg
     8161 acggccgcca ttattttcgg caccgaaccc aagaacaatt tccccgcccc cttctcgccc
     8221 agcgacgtca tcaagatccc cttccccggc gcattcatgg acgtgatcat cctgaaacaa
     8281 gccctgttgg ccacggccat cgtcgccgtc atcgccgccg tcatcttcat gatcttcttc
     8341 cagtatacgc gcatcggcct ggccatgcgg gccacatccg aagaccatga gctggcccgc
     8401 ggcgtgggca tcaacgtgcc gcgcgtgttt ggtctctcgt gggccatcgc cggcatcatc
     8461 gccaccgtcg gcggggtgtt gctggccact gtcaccggcg tcagcctcag cctggccacc
     8521 atcaccctgg tggctttccc ggccatcttg ctgggtggcc tggagtcgtt ttccggagcc
     8581 atcatcggcg gcctgctggt gggtcttgcc caggcgctcg tccaggcctc atcggtgcaa
     8641 acgatccgcg gctcgtcgga catcgcgcct tatatccttt tgctgattgt tctggtcatt
     8701 cgtccggaag gcctcttcgg cgaaaagcgg attgagaggg tctaaccgat ggctgcatta
     8761 cgacctgcgg gcgacttcga ccgcacttac gaacaggata tgggcgtcat tcgccggccg
     8821 tggcaatggc tggcgctggg cgtcggcttc ctgctgctcc tcactgcccc acgctgggcg
     8881 ggcgcttatc tggtctcgac tgccaaccgg attttcatcc tggtcatcgc cgtccagggt
     8941 ctcaacatcc tcgtcggcta cactggccag atctctctcg gccaggccgc gttcatgctg
     9001 gtgggtggct acacctcggc gctggtgacc agccatctat cggtaccttt cccactggcc
     9061 ttgctcatcg ccgggttggt gtccgggctg atcgggttgc tcttcggcct gccgtcgctg
     9121 cgggtcaagg ggttctatct ggctatggcg acgctggccg cgcaatttat catcccctgg
     9181 gtcagccgcc atctttaccc cgacctgctg ggcgggccgg ccggccgcat cagcgccgac
     9241 gtgccggtca tcttcggcat ctcgttcggc gaagtcggca actactacta cctttcgctc
     9301 atcaccctgg tgatcaccac cgtcgccgtc ctcaatatca gccgctcgcg gctggggcgg
     9361 gcgtttgtct ccctgcgcga taacgacctg gcggccgaac tgttgggcgt caacctgttc
     9421 gactacaagc tgcgggcgtt cttcattgcc tcgttcctgg ccggcgtcgc cggtgcgctg
     9481 cgcgcccaca gccagcgcgg cgtgggcacc gaacttggct acggcctggc ggaatcgatc
     9541 attatgctcg gtatgctggt gatcggcggt ctgggcacaa atctggggcc gtttctgggc
     9601 gtgaccatgg tggtattgct cgaagacacc gctggggtgg ccggcagcac catggccaag
     9661 ctgttcccga cgcaggcggc gcgcttcctg acatctttcc ggccgatctt tttcggcctg
     9721 gtgctgatgc tgttccttat ctttgaaccg cgcggactag cccatcgctg gcagactttc
     9781 aaggcggcct ggcgattgcg accgtttgcg cgttagctgc ttgtcgactg tgtttatttc
     9841 aatatgtgca agaggaggca acgcttattg aagattctgt aggttctttt catcggattt
     9901 agaggtttcg catcaaaaaa gacaggagat aatgagatga agaagatttc actggtttct
     9961 ctgctgctgg tcatggccat catgttggcc gcctgcggtg gggccgcgcc gactgttgaa
    10021 cagcttgccc cgaccgtcca gagtgtggtc gagcaagtcg cccccaccat cgccgccgtg
    10081 gcgacggaag tagccccgac ggttgccgcc gtcgcgacgg ccgtcgcccc cacagaagtg
    10141 ccgccgactg aagtgccgcc gaccgaagaa ccggccgcgc ccgaagtcat cagcggcgaa
    10201 tcgaccaacg acgactgcgc cgcgatgtac gacggcgagt cgatccacgt ccaccaggcc
    10261 gccggcctga ctggcccgct ggcccagatt ctgggcgacg gtttcatcaa tggctcgaag
    10321 gcggccgtcg atcgcatcaa
//
LOCUS       DAOMQG010000094        10282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2805_length_10282_cov_11.365894, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000094 DAOMQG010000000
VERSION     DAOMQG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_10282_cov_11.365894"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLC06_07110"
     CDS             <1..1406
                     /locus_tag="PLC06_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRO90251.1"
                     /translation="EVLAMVNLPTFDNNRFQTEVPVDYYLGLARNPYTPLVNHAISGT
                     YPPGSAFKLVPAAAALQEGVISPDRYLFDPGTIEIPNRFAPNDPGRVQPFVCWDLAGH
                     GLVNMRLAIKHSCDVYFYKITGGFDQDGEFVEGLTVDRIDLYGHQFGFGRVQGIELPL
                     EAEGNLPTRAWKRQTQGEPWSTGDDYNLGIGQGFMTATPMQQAQMAAVVANGGFLYRP
                     TIVHHMTDADGNIVITNDDGKIVARAHPDRNGNTILTDADGNPLDDPTINVKFDADGN
                     FIFQPEVIDTLAVDQQYLQVVREGMHLVNQHIVDTNEYGTGATYVDWNGLEAAGIPTA
                     GKTGTAEYCDNIAITRGWCRFEDIAQRRILPTHAWYVAYAPYDDPEIAVSVFVFNGGE
                     GSAWATPVACHVIAAYFGVAQYASLMGDLTPEEAAAQTPVCNSLIYNPALPQFDFGTP
                     SGTPPETTPGAIPLNRN"
     gene            1503..2306
                     /locus_tag="PLC06_07115"
     CDS             1503..2306
                     /locus_tag="PLC06_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HRO90252.1"
                     /translation="MGTLILVRHGENEWTRKGKLAGKIPGVHLNEAGHQQAQAAAKRL
                     AALPIKAVYSSPLTRCAETAAYIADTHRLTTLFVDDLGEVEYGEWEDKKIEKLARKPL
                     WRAVQFFPSRMRFPRGEALREVQFRAVQALEEIAARHEKEMVVVVSHADIIRLLLAYY
                     LGVHIDLFQRLVISPASASVLHLSADGMSRVLRFNDDGPLHSPSQPEGWKKDKKAGKK
                     RKETGRETGSPDKQDSKAGSQTTSGVSANGNKPNRALHPTSSAALDEEE"
     gene            2313..2828
                     /locus_tag="PLC06_07120"
     CDS             2313..2828
                     /locus_tag="PLC06_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HRO90253.1"
                     /translation="MAHQIDLNPASHLTVGTIGVPGQRTFYLQGSRGTQTVSLVIEKE
                     QAILLGSSLENFVEELERNHPSGKREPAEPVWMDMRLREPIDELFRVGNMGLGYSEND
                     DLIVLIAYELVEEGEDPNVVSFWVGRAQIQSLINHINDVVKQGRPICGNCGRPIDPEG
                     HFCPQRNGHRH"
     gene            2944..3693
                     /locus_tag="PLC06_07125"
     CDS             2944..3693
                     /locus_tag="PLC06_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HRO90254.1"
                     /translation="MTEPTTGEVLQALQQGTVEIEGMLSWSSNYAFLVQVCDGALELG
                     AVYKPARGERPLWDFPQNTLCLRERAAFVVSEAIGWHIVPPTVLRDAQHGPGSVQLFI
                     DHDPQQHYFTIENNEAFRPQLQRIALFDILINNADRKAGHVLIGQDEDGDKPARLWGI
                     DHGIGFHVDPKLRTVIWEFGGAPIPAEMKHEMEAFRERLGDESLDLNIELVKLLSPDE
                     VEAMQRRLDRLINRTTFPQPGAGRHYPWPPV"
     gene            3717..4577
                     /locus_tag="PLC06_07130"
     CDS             3717..4577
                     /locus_tag="PLC06_07130"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HRO90255.1"
                     /translation="MSTDALIDQAIELLGEARHAVALTGAGMSTPSGIPDYRSPKSGL
                     WEKEADMMEVASIYGFRHHPQAFYDWLRPLLEVIRSARPNPAHVALAQLEAAGRLQAV
                     ITQNIDLLHGRAGSNKLYEVHGHLREMVCPACHHMLPADPLLDDFVATGKIPRCVRCH
                     HIMKPNVVLFGELLPWKTMKAAQAHARAADLMLVAGSSLEVAPAGDLPELAVENGARL
                     IIINFMETHLDSRADIIIRADLAEALPRLARPFLSPAPQPIDYPFEPPAGLIGKNPAT
                     GDVRMSGQAP"
     gene            4670..5602
                     /gene="trxB"
                     /locus_tag="PLC06_07135"
     CDS             4670..5602
                     /gene="trxB"
                     /locus_tag="PLC06_07135"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRO90256.1"
                     /translation="MQKERVVIIGSGPAGLSAAIYTARALLNPVVITGTQLGGQISLT
                     SEVENYPGLYDPEVTPTGPQMVEIMQNQAEHFGARLVYDEVVEVDFRSGPPFTVRTHG
                     ETYSADAVIVTAGASPKHLEVPGEVEFTGRGVSYCGTCDGFFFRGKDVVVIGGGDSAM
                     EESIFLTKFADKVTVVHRRDELRAGPTLQKRAFSNEKIDFVWNSVIEEIAGNGVVQGV
                     KLRNVETYEETEMPTDGVFIFIGHYPNSQFLTGQVAMDEHGYVITDEFMRTSVIGVYA
                     AGEIQDPYYRQIATSVGQGVAAAIQLERWLAEQE"
     gene            complement(5656..6957)
                     /locus_tag="PLC06_07140"
     CDS             complement(5656..6957)
                     /locus_tag="PLC06_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90257.1"
                     /translation="MIDRMGRSRISRIWLLFRLVLIVGILVVVVAPEWPAFGDAKFRF
                     QTIIGRQQRFDFIEWTAAALDNKWQAIRSGAQNAMSEEERHAFVLDYLDQLGTVMRIE
                     ADIERVFADPTVSDPDAATAGLRAERDALRKALSRRQATAEAIVQQQAAAILAEEGMD
                     IGGMAWPPVMMTMSPVPYLLVVSPRDRIAQIDSAALVPGLTTEDKEAMEDAVFDRLDQ
                     SALVVPIGGMGTYPAMIRETSSINRLADVVAHEWTHHWLDLKPLGMNYMTDPDLRIIN
                     ETVASIVDLEVGPRIIERYYPEHVLAPPVATPPTQPPEKPAFDYSAEIAETRNTVDRL
                     LAAGEITQAEDYMEQRRRVFLDNGYNIRKLNQAFFAFYGGYAAEPGGAAGEDPIGPML
                     REIRAASPTLRDFLLQVGEITSLDELRTLHAALVDARTASQ"
     gene            7205..9022
                     /locus_tag="PLC06_07145"
     CDS             7205..9022
                     /locus_tag="PLC06_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90258.1"
                     /translation="MGLQKATRYPSKNPEEAITPVSRSSVKTAVRLLGYVRPYWRWMA
                     LTIFALTVSSLLGLVLPLVVRNLVDYVIVNKGINDLNLVTIGMLVVFLLQAVFSFIQH
                     ITLAYTGEHAMADIRISIFTHLQSLPLSFYADRRTGELVSRLTNDVSLLQQALTVNLA
                     TLMGMVITVIGAAVLLFWLDWRLTMLIMFAVPVITLTMVWLGNRVRRESVAVQDALAE
                     VANSAEETTRGARIVKSFAREPYEIGRFTNHIMTLYRAAMRRARVHSLLSPLIGLIAS
                     VTITAIIYYGGVQVLRGNLSPGDLMAYLIYTVMIAAPIAIIAELYGQFQSALGASERI
                     FELLDQPSELVELPDAVPLPPVAGEIAFDNVSFKYTSTTDVINDVTFRVEPGQVIALV
                     GPSGAGKSTLVNLLPRFYDVIDGSITIDGHDLRHVTLKSLREQIGIVPQETILFSGTV
                     YDNIRYGRLDATRKEVEAAAMAANAHSFIVEDLENGYDTAVGEHGVKLSGGQRQRVAI
                     ARAILKNPRILILDEATSSLDSESESLVQEALERLMSGRTSFVIAHRLSTVLNADRIL
                     VLSKGEIVEQGDHHTLLLDPDGLYTRLYHMQFAASEAMP"
     gene            complement(9126..9293)
                     /locus_tag="PLC06_07150"
     CDS             complement(9126..9293)
                     /locus_tag="PLC06_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90259.1"
                     /translation="MSWFLLFLGASVFSSIFVLAAGMLSSRMNRREDIVESFYMDASE
                     ATIPSGARITE"
     gene            complement(9504..10277)
                     /locus_tag="PLC06_07155"
     CDS             complement(9504..10277)
                     /locus_tag="PLC06_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRO90260.1"
                     /translation="MTYENILTRIEGRAGVVQLNRPKALNALNRELMTELMDALEAYD
                     RDPAVGCMVITGSERAFAAGADIKEMATATPASMMTNSFIDLWDRLRVMGKPIIAAVS
                     GFALGGGCELAMTCDMIVAAETAQFGQPEINLGVIPGAGGTQRMTLAVGKAVAMEMVL
                     NGRYLSAAEAQHYGLVNRVYPVEVYLDEAIRLANEIAARAPVAVRLAKEAVNATFEMP
                     LRAGLDHERRLFYLLFGTADQKEGMDAFVSKRKPQWTGG"
BASE COUNT         2067 a         3266 c         2991 g         1958 t
ORIGIN      
        1 gcgaagtgtt ggccatggtc aacctgccga cattcgacaa caaccggttc cagaccgaag
       61 tcccggtcga ttactatctg ggcctggcgc gaaatcccta cacgcccctg gttaatcacg
      121 ccatcagcgg cacctacccg cctggctccg cgttcaagct cgtccccgcg gcggcggcct
      181 tgcaagaggg cgtcatctcg ccggatcgct acctgtttga ccccggcaca attgagattc
      241 ccaaccgctt cgccccgaat gaccccggac gcgtgcaacc attcgtgtgc tgggacctgg
      301 ccgggcatgg tctggtgaac atgcgcctgg ccatcaaaca ttcgtgcgat gtctatttct
      361 ataaaatcac cggtggattc gaccaggatg gcgagttcgt tgaggggctg accgtcgacc
      421 gcatcgatct ctatggccac cagtttgggt tcggccgcgt ccagggcatt gaactgccgc
      481 tcgaggcgga gggcaacctg cccacgcgcg cctggaagcg ccagacgcaa ggcgagccgt
      541 ggtccacggg tgacgactat aacctgggca tcggccaggg tttcatgacc gccacgccga
      601 tgcagcaggc ccagatggcg gccgtcgtcg ccaacggtgg cttcctctat cggccgacta
      661 tcgtccacca tatgaccgac gccgacggaa atatcgtgat taccaacgac gacggcaaaa
      721 tcgtcgcccg cgcccacccg gaccgcaacg gcaataccat cctcaccgac gcggacggga
      781 acccgctgga cgatccgacc atcaacgtga aattcgacgc cgacggcaat ttcatctttc
      841 agcccgaagt tatcgacacg ctggccgtcg accagcagta cctgcaagtc gtccgcgaag
      901 ggatgcacct ggtgaaccag catatcgtgg atacgaatga gtatggcaca ggggcgacct
      961 acgtcgactg gaacgggctg gaggcggcgg gaattcccac cgcgggcaag accggaacgg
     1021 ccgaatactg tgacaatatt gccatcaccc gcggctggtg ccgatttgag gacatcgccc
     1081 aacggcgcat cctgcctact cacgcctggt atgtagccta cgcgccctac gacgatccgg
     1141 agatcgccgt gtccgtcttt gtgttcaacg gcggcgaggg atcggcctgg gccacgcccg
     1201 tcgcctgcca cgtcatcgcc gcctacttcg gcgttgcgca atatgcgtcg ctcatgggcg
     1261 atctgacccc cgaagaagcg gccgcgcaga cgccggtatg caactctctc atctacaacc
     1321 ccgcactacc gcaattcgac ttcggtaccc cgagcggaac cccgcccgag accaccccgg
     1381 gcgctatccc cctgaacaga aattaagcct gatcctttac ctacccgtgg cacaggcgtt
     1441 tcgccgatag aaggacctgt ccgtcaatcg tagcgccctc ttttttgtgc tatcattttc
     1501 gcatgggaac cctcatcctc gtgagacatg gcgaaaacga atggaccagg aagggcaagc
     1561 tggcgggcaa gattccgggc gttcacctga atgaggccgg gcaccagcaa gcccaggcgg
     1621 ccgccaagcg tttggcggct cttcccatca aggccgtata cagcagcccg ctgacgcgct
     1681 gtgcggagac agccgcctac atcgccgaca cccatcgcct gacgaccctc tttgtcgacg
     1741 atctgggcga ggtggaatat ggagaatggg aagacaagaa gatcgagaaa ctggcccgga
     1801 agcccttgtg gcgagccgtc cagtttttcc ccagccggat gcgcttcccc cggggcgagg
     1861 ctttgcgtga agtccagttc cgtgccgtcc aggctctgga agagatcgcc gcccggcacg
     1921 agaaagagat ggtcgtcgtc gtttcccatg ccgacatcat ccgcctgttg ctggcctact
     1981 atttgggcgt ccatatcgat ctgtttcagc gccttgtcat ttctccggcc tcggccagcg
     2041 tgctgcacct gagcgcggac gggatgagtc gtgtgctgcg cttcaacgac gacggcccgc
     2101 tgcactcccc gtcacagccc gaaggctgga aaaaggataa gaaggccggc aagaaacgca
     2161 aagaaaccgg ccgggaaacc gggtcgccgg acaagcaaga cagcaaggcc ggctctcaga
     2221 ctacgagcgg cgtatcggcc aacggcaata aacccaatcg agcgctccac cccacctcat
     2281 ccgccgcgct cgatgaagaa gagtaatcat tcatggccca tcaaattgat ttgaatccgg
     2341 cgtcccatct gactgtggga accataggtg tgcccggaca acgaaccttc tacctgcaag
     2401 gcagccgggg cactcagacc gtctcgctcg tgatcgagaa agagcaggcg atccttctcg
     2461 gcagcagcct ggaaaacttc gtcgaagagc tggagcgcaa tcatccttcc ggcaagcgcg
     2521 agccggccga gccggtatgg atggacatgc gcctgcgtga gccgatcgac gagttgttcc
     2581 gcgtgggcaa tatgggattg gggtatagcg agaacgacga tctgatcgtc ctcatcgcct
     2641 atgaactggt ggaggaggga gaagacccta acgtagtcag cttctgggtc ggccgggcgc
     2701 agatccagtc gctcatcaac catatcaacg atgtcgttaa acaagggcgg ccgatctgtg
     2761 gcaattgcgg ccgtcccatc gatcccgaag ggcatttttg cccgcagcgc aatggccacc
     2821 gccactagat cgcgatcttg tcgcttagcg actgctgtcc gattatgacc atttggtgac
     2881 catcgcatga taactatcgc gcgataacca tcgcacgata accatcgcac gataaccatc
     2941 gccatgactg agccgacgac aggagaggtg ctgcaagccc tgcaacaggg tacggtggag
     3001 atcgagggca tgttgtcctg gagttccaac tacgccttcc tcgtgcaggt gtgtgacggc
     3061 gcgctggaac tgggcgccgt ctataagccg gcgcggggcg agcggccgtt gtgggacttt
     3121 ccgcaaaaca ccctctgcct gcgcgaacgg gcggccttcg tcgtcagcga ggccatcggc
     3181 tggcatatcg tcccgccgac cgtcttgcgc gacgcgcagc atgggccggg gtctgtgcag
     3241 cttttcatcg accacgaccc ccaacaacat tatttcacca tcgagaataa cgaggcattt
     3301 cggccccaac tgcaacggat tgctctgttc gatatcctga tcaacaatgc cgaccggaag
     3361 gccggccacg tcctcatagg gcaggacgag gacggcgaca aacccgcgcg tctgtggggc
     3421 atcgatcatg gtattggctt ccacgtcgat cccaagctgc gcacggtgat ctgggagttt
     3481 ggcggcgcgc caatcccggc cgaaatgaag cacgagatgg aagcgttccg cgaacggttg
     3541 ggagatgagt cattggattt aaacatcgag ctggtcaaac tgttatcgcc ggacgaggtt
     3601 gaagccatgc aacggcggct cgaccggctt atcaaccgga caactttccc ccagccgggc
     3661 gcgggacgtc actacccctg gccgccggtt tgatcgacac aacgggaatg tattgaatgt
     3721 caacggatgc tttgatcgac caggcaattg agttgttggg cgaggcccgt cacgccgtcg
     3781 ccctgaccgg cgcgggaatg tccacgccct cgggcatccc cgactatcgc agcccgaaat
     3841 ccggcctctg ggaaaaggag gccgacatga tggaagtcgc cagcatctac gggttccgcc
     3901 atcatcccca ggcgttctac gattggctcc ggcccctgct cgaggtgatt cgcagcgctc
     3961 ggcccaaccc ggcccacgtt gccctggctc aactggaagc ggccggccgt ctgcaggcgg
     4021 tcatcaccca gaacatcgac ctgctccacg gccgcgcggg gtcgaacaag ctctatgagg
     4081 tacacggaca tctgcgcgag atggtttgcc cggcttgcca tcatatgctc ccggccgatc
     4141 cgcttctgga tgactttgtg gctaccggca aaatcccgcg ttgcgtgcgc tgccaccata
     4201 tcatgaagcc caatgtcgtc ctgttcggcg agctgctgcc gtggaagacg atgaaggccg
     4261 cccaggccca cgcccgcgcg gccgatctga tgctcgtcgc cggctcatcg ctggaagtgg
     4321 cgcccgccgg tgacctgccg gagctggcgg tggagaacgg cgcgcgtctc atcatcatca
     4381 acttcatgga gactcacctc gacagccggg ccgatatcat catccgcgct gatttggccg
     4441 aggcgttacc ccgactggcc cgcccgttcc tgtcgcccgc gcctcaaccc atcgattacc
     4501 catttgaacc gcctgccggc ctgatcggta agaatccggc caccggtgac gtcagaatga
     4561 gcggacaggc gccatagcag gcgcgcatta aattgaaaac tgtgcggggg agcacagtgg
     4621 cgaagcacaa taacgcccgt ccccaccgct gaatcaaacg agaaaagata tgcaaaagga
     4681 acgagtcgtt atcatcggat ccggcccggc gggattatcg gccgccatct acactgcgcg
     4741 ggcgttgctg aaccccgtcg tcatcaccgg cacccaactg ggcggccaga tttccctgac
     4801 cagcgaagtg gaaaactatc cgggcctcta cgaccccgaa gtgactccca ccggcccgca
     4861 gatggtggaa atcatgcaaa atcaggccga gcattttggc gcgcgcctgg tgtatgacga
     4921 agtggtcgag gtcgacttcc gcagcggccc gccgttcacc gtccgaactc acggcgagac
     4981 ttattcggcc gacgccgtca ttgtgaccgc cggggcatcg ccgaagcatc tggaagtccc
     5041 cggcgaggtg gaattcaccg gccgcggcgt cagctattgc ggcacatgcg acggcttctt
     5101 cttccgcggc aaggacgtcg tcgtcattgg cggcggcgac agcgccatgg aagagagcat
     5161 cttcctgacc aagttcgccg acaaggtgac tgtcgtccat cgccgcgacg agctgcgcgc
     5221 cgggccgacg ctgcaaaagc gcgccttctc caacgagaag atcgattttg tctggaacag
     5281 cgtcattgag gagatcgcgg gcaacggtgt cgttcagggg gtcaagctgc gcaatgtgga
     5341 gacatatgaa gagacagaga tgcccaccga cggcgtcttc atcttcatcg gtcactaccc
     5401 aaacagccaa ttcctgacgg gccaggtcgc catggacgag cacggctacg tcatcaccga
     5461 cgagttcatg cgcaccagcg tcatcggcgt ctatgccgcg ggcgagattc aggatcccta
     5521 ctaccggcag atcgccacgt cggtcggcca gggtgtggcc gcggccatcc aactggagcg
     5581 ctggctggcg gaacaggaat aggcaggatt ggcgaaacag aagcccggcc ctcgaatccg
     5641 ggagccgggc tttttttatt ggctcgccgt cctggcgtct acgagcgcgg catgcagcgt
     5701 ccgcaattca tcgagtgaag tgatttcgcc cacctgcagc aggaagtctc gcagcgttgg
     5761 gctggcggcg cggatctcgc gcagcatcgg accgatgggg tcttcacccg ctgcgccgcc
     5821 cggctcggcc gcatagccgc catagaaggc gaagaaggcc tgattcagct tgcggatgtt
     5881 gtagccgttg tccagaaaca cacggcggcg ctgctccata taatcctcgg cctgcgtgat
     5941 ttcacccgcg gccaatagtc ggtcgaccgt gttgcgcgtc tcggctattt cggccgaata
     6001 atcgaaggcg ggtttctccg gtggttgcgt tggcggcgtg gccaccggcg gcgcgaggac
     6061 atgctcagga taatagcgtt cgatgatccg cgggccgacc tccagatcga cgatcgacgc
     6121 caccgtctca ttgataatgc gcaggtccgg atcagtcata tagttcatac ccagcggctt
     6181 caagtccagc caatggtggg tccattcgtg cgccaccaca tcagccaacc ggttgatgct
     6241 cgatgtctcg cggatcatgg ccggataggt gcccattccg ccgatgggga cgaccaacgc
     6301 cgactgatcc agccggtcga acacggcatc ttccatcgct tccttgtctt cggtggtgag
     6361 gccgggcacg agcgcggccg aatcaatctg ggcaatgcga tcgcgcggcg aaacgacgag
     6421 tagatagggg acgggcgaca tggtcatcat caccggcggc caggccatcc cgccgatatc
     6481 cataccctct tcggcgagga tcgccgccgc ctgctgctgg acgattgctt cggccgtggc
     6541 ttgtcgtcgg gaaagcgctt tgcgcagggc gtcacgctcg gcccgcaagc cggctgttgc
     6601 cgcgtcggga tcgctcaccg tcggatccgc gaagacgcgc tcgatgtccg cctcaatgcg
     6661 catgaccgta cccaattgat ccaggtaatc gagcacgaac gcatggcgct cctcttcgga
     6721 catggcgttt tgggcgcccg accggatggc ctgccatttg ttgtccagcg ccgcggccgt
     6781 ccactcgatg aaatcgaatc gctgttggcg gccgatgatg gtctgaaagc gaaatttggc
     6841 gtcgccgaaa gccggccatt ccggcgcgac gacgaccacg aggattccga cgatcagcac
     6901 gaggcgaaac agtaaccaga tacgcgagat acgactcctg cccatgcggt cgatcatatg
     6961 tcggccgcat tagcctgacc acttatcccc gctccgcttc accaacatct aacccgctgc
     7021 cacaggttag aaacccattg caataagaca cgtattcttc attagcctct acaattagca
     7081 ggtcgttgtg aatgtgtctt gtcccgatcg gtcgttgcac ttctggctga tcgtgacgaa
     7141 cggcgactaa ccggctaaaa tctgaacgac cataacgaat cattctctgg gaaggtaaac
     7201 cctcgtgggc ttacagaaag cgactcgcta cccatccaaa aaccctgaag aggcaatcac
     7261 acccgtctcg cgcagcagcg tcaaaactgc cgtgcgattg ctgggttacg ttcgccccta
     7321 ttggcgttgg atggcgctga ctatttttgc tctcacggtc agttccttgc tcggcctcgt
     7381 cctgccgctc gtcgttcgca acctcgtcga ttacgtcatc gtcaacaagg gaatcaacga
     7441 cctcaatctg gtgacaatcg gcatgctggt ggtcttcctg ctgcaagccg tcttctcttt
     7501 cattcagcac atcaccctgg cctacaccgg cgaacatgcc atggccgaca ttcgtatcag
     7561 tatcttcacc catctgcaat ccctgccgct cagcttctat gccgaccgcc gcacgggcga
     7621 actggtctcc cggctgacca atgacgtctc tctgctgcag caagcgctaa ccgtcaatct
     7681 ggccacgctc atggggatgg tgatcaccgt catcggcgcg gcggtgctgc tattttggct
     7741 cgactggcgg cttaccatgc tgatcatgtt cgccgtgccc gtcatcacgc tgacgatggt
     7801 gtggctgggc aaccgcgtgc gccgtgagtc ggtcgccgtc caggacgctc tggccgaggt
     7861 cgccaattcg gccgaagaga cgactcgcgg ggcgcgcatc gtcaagtcgt tcgcccgcga
     7921 gccgtatgag atcggccgtt tcaccaacca catcatgacc ctctaccgcg ccgcgatgcg
     7981 acgggcgcgc gtccactcgc tgctaagccc gctcatcggg ctgattgcct cggtcaccat
     8041 cacggccatc atctattacg gtggcgtaca ggtgctgcgg ggcaatctct cgcccggcga
     8101 tctcatggct tacctcatct acaccgtgat gatcgccgcg cccatcgcca tcatcgccga
     8161 gctttatggc caattccagt cggctctggg cgcgtcggag cgaatctttg agttgctgga
     8221 tcaaccgtcg gagcttgtcg agttgcccga tgccgtgccg ctgccgccgg tggcgggaga
     8281 aattgcattc gataacgttt cgttcaaata cacctccacc accgacgtga tcaacgacgt
     8341 cacttttcgg gttgagccgg ggcaggtcat cgccctggtc ggccccagcg gcgcgggcaa
     8401 gagcacgctg gtcaacctgt tgccccgctt ctacgatgtc atcgacggca gcatcacgat
     8461 cgacggccac gacctgcgcc atgtcacgct taagagcctg cgcgagcaga tcggcatcgt
     8521 gccgcaagag accatcctgt tctccggcac ggtgtatgac aacattcgct atggccggct
     8581 ggacgcgacg cgcaaggaag tggaagccgc agccatggcc gccaacgccc acagcttcat
     8641 cgtcgaggac ctggagaacg gttacgacac ggccgtcggc gagcatggcg tgaagttgag
     8701 cggcggccag cgccagcgcg tggccatcgc ccgggccatc ctgaagaacc cgcgcatcct
     8761 catcctcgac gaagccacct catcgctgga cagcgagagc gagagcctgg tgcaggaggc
     8821 gctggaacgg ctgatgagcg gccgcacgtc gttcgtcatc gcccaccgat tgagcaccgt
     8881 gctgaacgcg gaccgaatcc tggtgctgag caagggcgag atcgtcgagc agggtgacca
     8941 ccacacattg ctgcttgatc ccgacggcct ctacacgcgc ctgtatcaca tgcaatttgc
     9001 ggcttcggaa gcaatgccgt agggagctgc ttcgcggctg cgaaaaaaca aaacagccgc
     9061 ccgaattggg cggctgcctg atgtcaacag acttccttct ctaaacaaaa gctggacggt
     9121 ttgatttact cagtgatccg ggcgccggac ggtatggtgg cttcgctagc gtccatgtag
     9181 aacgactcaa cgatgtcttc ccgccgattc atccgcgagg acaacattcc cgccgccagg
     9241 acgaagattg agctaaaaac cgaggctccc agaaaaagta aaaaccagct cattttgcct
     9301 acctctacgc tattggttac agcttttcag gaattcccgt caccgtaaga aacttcgtaa
     9361 gaaagcaatg ctacaatacg tgcgtcgatg atgtttgata ttatagtggc tttttcctcg
     9421 cttgtctacc caataccgtg aagaacgggt gacaaattgg ttataacttt agtactactc
     9481 atctacgagt agtggattat tacctaaccg cccgtccatt gcggcttgcg cttgctcacg
     9541 aatgcatcca tcccctcttt ctggtcggcc gtgccgaaca gcaaatagaa caaccgccgc
     9601 tcatggtcca gcccggcgcg caacggcatc tcgaaggtgg cattgaccgc ctccttggcc
     9661 aggcgcacgg ctaccggcgc gcgcgcggcg atctcgttgg ccagccggat ggcctcatcg
     9721 agatagacct ccaccggata gacgcggttg accaggccat aatgctgcgc ctcggccgcc
     9781 gacagatagc gcccgttcag caccatctcc atcgccaccg ccttgccgac ggccagcgtc
     9841 atgcgctgcg tgccgccggc gccggggatg acgcccagat tgatctccgg ctggccgaac
     9901 tgcgccgttt cggcggcgac gatcatgtcg caggtcatcg ccagctcgca gccgccgccc
     9961 agggcaaagc cggatacggc ggcgataatc ggcttgccca tcactcgcag gcgatcccac
    10021 agatcgatga aggagttggt catcatcgac gcgggcgtgg ccgtagccat ctccttgatg
    10081 tcggccccgg cggcgaaggc gcgctcgctg ccggtgatga ccatgcagcc gacggcgggg
    10141 tcgcggtcat acgcctccag cgcgtccatg agttcggtca tcagctcgcg attcagcgcg
    10201 ttgagcgcct tcggccggtt caactgcacg acgccggcgc gcccctcgat gcgggtcaga
    10261 atgttctcgt aggtcatcat ac
//
LOCUS       DAOMQG010000095        10242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2826_length_10242_cov_10.281143, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000095 DAOMQG010000000
VERSION     DAOMQG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2826_length_10242_cov_10.281143"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1034)
                     /locus_tag="PLC06_07160"
     CDS             complement(63..1034)
                     /locus_tag="PLC06_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRO90261.1"
                     /translation="MVIILGDILADYAIRIERLEIHPKDLQRVSYLGLGPGGACNVAI
                     MASHLGLPVGALGEVGKDLFGEIVLEGLAAEGVDVSRVAVTPNARTPVANVLVDEQGE
                     PAYLGFPGSLQIMSLPDEWRPFIAEADALYADGWVDQPGEADIIMAGLRQAREANVPS
                     FFDPGPGNPKIDNAWHTEAAAIATVLLATEEEARRLSGESDPVNSARALLTRGSNLVV
                     IKRGMAGCFLLTENDLHIAPGLPVAALDATGAGDSLAAAVIYGYLNKFNLQALGDLAN
                     ATGAAKVLKLGTGHNMPTVDEVNAMLRRFGSSVALSPGTSIPDNSQA"
     gene            complement(1051..1866)
                     /locus_tag="PLC06_07165"
     CDS             complement(1051..1866)
                     /locus_tag="PLC06_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90262.1"
                     /translation="MKLSIEDIAFTYPGGVEALRGVSINIRSGEAVAIVGENGAGKTT
                     LVKHINGLLKPARGRVVVGDWDTREHGVARMAARVGFVFQNPDDQIVERTVGDEVAFG
                     PRQLGQSAPMALANATAALEMVELAAESGTHPYDLTAPQRKLVTLASVLAMHTPIVIF
                     DEPTMGQDAHGVALIGNIIESLKADRRTVIIITHDIDFCAAHFERVIAMADGCVLADG
                     PATRVLARADLLATTNVEPPQLVRLAQAIGLPAFPLTVEGFVTALLEHHSKDK"
     gene            complement(1863..2699)
                     /locus_tag="PLC06_07170"
     CDS             complement(1863..2699)
                     /locus_tag="PLC06_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90263.1"
                     /translation="MISLSDVTYHYPRSDSPALSNLSLEIPAGQFCAVIGANGSGKST
                     LCSALAGFVPHFYRGKLTGSVEVAGIDTAGSSLAELAGTVGLVFANPFNQITGARFTV
                     REEVAFGLENLGVPLAEMEPRIDEVLAFVRLTDLADQSPYALSGGQQQRLAIASVLVM
                     RPKILVLDEPTSQLDPIGSKEVFAVLRDLAATDEATVVLAEQKLEWVATFADRVLVLH
                     KGKLVADGPPAEVLSSLTLTKYGLEPTRYTQAARAAQAAGWRTDETTLPVTLEQAVEF
                     FR"
     gene            complement(2696..3493)
                     /locus_tag="PLC06_07175"
     CDS             complement(2696..3493)
                     /locus_tag="PLC06_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRO90264.1"
                     /translation="MSATYGLYVSKQTAIHRLHPLTKLTYVLTCLLIAWIVPGVWAPY
                     IVLLVGIVPVALMAQVFPNVVQNTIKTTIPFAISLFIVQGLFWTGGTIIQQLGPFSLK
                     EEGLLFAIRGTGRIIIAVSGFLLLSYATRSDELMLALSQKGLPSRISYVILSTLQIIP
                     RFQHKANTILDAQRSRGLETEGSFLIRTRALLPLVFPLLLGSIVDIEERAIALEVRAF
                     GRSGPKTSLLVLHDTNRQRLFRRLLILVSLGVIALRIYLWLAPRFAA"
     gene            complement(3514..4227)
                     /locus_tag="PLC06_07180"
     CDS             complement(3514..4227)
                     /locus_tag="PLC06_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRO90265.1"
                     /translation="MGPKVGLKMALERGANDSYRVSYRLPGKPERVIPKAGGWRKMEK
                     LKRDFNTIALAMIPIAIAINIAVGQIVKALRIPLYLDSIGTVFVGAVAGPWVGAVAGL
                     LSNIVWAFIFGDVTNTVPYAPVAAAIGFLAGVFGKYGWFKSWWKAVLGGAITGVVASL
                     LSAPITFYLFGGVVGTGMDVITAAFRSQGMSMLAANFLQGLATDPLDKAISFLVVYLI
                     IRTLPDRYIDRFSRADDIR"
     assembly_gap    4497..4596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4611..7754
                     /locus_tag="PLC06_07185"
     CDS             4611..7754
                     /locus_tag="PLC06_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRO90266.1"
                     /translation="MFRKNSLKRSIKLLVVLGILALTASVAFAQDRPWTTQGERGNYS
                     ERPMPAQISGAKLDTPAQMQVENLSDLRVNPALRGQAGRVQVVVRLAGASATNGPTLQ
                     AAVSQQGAFVSAARAVDSRAKVLGSTQVVMNAVMLEVDASALSALAQNPDVVSINPVR
                     NYELDLSETVPYIGATAVQAAGFDGTGVRVAVIDSGIDYTHIAFGGSGDPNDWLNNDP
                     TIIEPGTFPTARVVGGYDFVGSEWPNGPEMPDPDPIDDGPGGGHGTHVADIIGGAMGV
                     APGVDLYALKVCSSVSSSCAGVPLIQAMEFAVDPNGDGNPDDAVDVINMSLGSSYGDP
                     RWDDLSYAVNNASALGVLTVASAGNSADKPYANGTPSSASTALAVAQTNVPSANQPMM
                     EVTAPASIAGLYAAVFQPWAVEPTARIEGPLQYGDGAGGNLNGCTSFAPGTLTGKIVL
                     VDRGTCGFSLKISNIADGGALVGIIGLIAPGDPFQGGFGEGDPSIPGYMISQADSNAL
                     KSGLAEGVTVVFDPANGISLVQHLVGSSSRGPTVGTNEAGPDIGAPGASVSAVWGSGT
                     GTEAFGGTSGAAPMVTGSAALLMSAYPDRTWSEIKAVLMNTGETDMMNNPAFFGGYQA
                     AISRIGGGEVRVFDALNSTVAAWDRNALDATLSFGFHDIVGNQTMVKRVTVRNYSNQP
                     VTLRSNVVYRYDNDDTGVVRLSVPSSFTVPRNGSVTFPVHMHIRPRTNQPLHDWVLNS
                     DSNGANPDALTLNEYDGYINFEGVGGSTASIHMPWHVLPRGAGNIQSGVRQNGLNWVR
                     NTGVAASYIDTYSLIGMSDQQPDAPVLGGNAAPADLRYVGVQTYPVPAGYCGAEPSFV
                     MAFAVNTWDRYSHAAANIWIEADVDVDGDGLADYAIFNFDNSGGFSSAPSDGRAVTWV
                     YDLSTGSADAWFYATHETNSGNVVLTFCGDQIGLNANDFFTTSMGVDVFANDWYYSGG
                     ADAITGLNVVPLGERYYTIFGNGDAGLTELPARSGALPFGVIDFGDQMNATENGLLWI
                     YGPGAPADNEAQAWIIN"
     gene            complement(7946..>8692)
                     /locus_tag="PLC06_07190"
     CDS             complement(7946..>8692)
                     /locus_tag="PLC06_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRO90267.1"
                     /translation="LFRPETRICLIETPSNPGLTLVDIAQVAAMCRERDVYLVVDNTF
                     ATPIVQRPIELGADLVIHSTTKFLDGQGRTIGGVILGDSFIMRELTQFARQTGPTASP
                     FNSWIVSQALETLPLRMERHCANALTLANWLEGRAGVESVRYPFLASHPQVDLARRQM
                     SAGGGLVTFTVAGGLAAGRRFLDALRLCDIVANLGDARTTVTHPASTTHSSLSEEDRL
                     ATGITPGLVRVSVGLEHIDDIIADIDQALG"
     assembly_gap    8693..8792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8793..8986)
                     /locus_tag="PLC06_07195"
     CDS             complement(<8793..8986)
                     /locus_tag="PLC06_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRO90268.1"
                     /translation="MTDLKSAHIETRLIRERTAIGPHREQITPIYLASGFAFPDAETA
                     RAVFSGEKPGYAYSRWGNPN"
     gene            9168..10136
                     /locus_tag="PLC06_07200"
     CDS             9168..10136
                     /locus_tag="PLC06_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRO90269.1"
                     /translation="MMEATVPLSAAWRTDPGLVRSHNEDFVIVHEPESPDDYQLHGAL
                     YIVADGVGGADAGEVASQYASEQTLHHYLQPIQFDTLGARLIEAMQSANTDLRRLAAE
                     RDDSRRMATTMVAVAVQDGHAYIGNVGDSRAYLWRRGVISQVTRDQSLVARLVEEGAL
                     TAEEALRYPYKNVILYSLGSERRPPIDLFEVELELGDLLLLCSDGLTRHVPDEEIARI
                     IAGEELDAAVETMVRLARQRGGEDNITVAIVQHGPREAALPEIDAEAAMLTIARPRPD
                     VQTAIRALPVAAEPHPRMWPLLLVLTTAMVVMIFLLWLFIQRVTTI"
BASE COUNT         1915 a         3056 c         3092 g         1979 t
ORIGIN      
        1 gatttcttcc gcaagatagt gctggtcttc ctgttgattc tgggtataca gttgattgtg
       61 gcctaagcct gggaattgtc tgggatgctt gtgcccggcg acagcgccac actggagccg
      121 aagcggcgca acatggcgtt gacctcgtcg actgtgggca tattgtgacc tgtgccgagt
      181 ttgagcacct ttgccgcgcc ggtcgcgttg gccagatcgc ccaatgcctg aagattgaat
      241 ttgttcagat agccgtaaat cacggccgcg gccaggctgt cgcccgcgcc ggtagcatcg
      301 agagcggcca ccggcaggcc cggcgcgatg tggagatcgt tctccgtcag caggaagcat
      361 ccggccatgc cgcgcttgat gacgacgagg ttgctgccgc gagtcagcaa cgcacgcgcg
      421 gagttgaccg gatcgctctc gccggagagc cgccgcgctt cttcttccgt ggctaacaga
      481 acggtggcga tagccgctgc ctcggtgtgc caggcgttat cgatttttgg gttgcccggg
      541 ccgggatcga aaaatgacgg gacgttggcc tcgcgtgcct gtcggagtcc ggccatgatg
      601 atgtccgcct cgcccggctg gtcgacccag ccgtcggcat agagcgcgtc cgcttcggcg
      661 atgaatggcc gccattcgtc gggcaggctc atgatttgca agctgccggg gaatccgaga
      721 taggccggct cgccttgctc gtcgaccagc acgttggcca ccggtgtgcg ggcgttgggt
      781 gtgacggcca cccgcgacac atcgacgcct tcggccgcca gcccttccag gacgatctcg
      841 ccgaagaggt ccttgcccac ctcgccgagc gcgccgaccg gcagacccaa atgggaagcc
      901 atgatggcaa cgttacacgc gccgcccggc cccaacccca gataggagac gcgctgcaaa
      961 tccttgggat ggatctcaag ccgttcgata cggatggcgt agtccgccag aatatcaccg
     1021 aggatgatta ccatgagaac tccgaatact ctatttgtct ttgctgtggt gctcgagcaa
     1081 tgcggttacg aatccttcga ctgtcagagg gaaagcgggc aaaccaatcg cctgggccag
     1141 ccggaccagc tgcggcggct cgacattggt tgtagccagc aggtcggcgc gcgccagcac
     1201 ccgggtcgcg ggaccatcgg ccagcacgca accgtcggcc atggctatga cgcgctcgaa
     1261 atgagccgcg cagaaatcaa tgtcgtgggt gatgatgatc accgtgcgcc tgtcggcctt
     1321 gagtgactcg ataatgttgc cgatcagcgc cacgccgtgc gcatcctggc ccatggttgg
     1381 ctcgtcgaag atgacgatgg gcgtgtgcat ggccagcacg gaggccagag tcaccagctt
     1441 gcgttgcggc gcggtcaggt cataaggatg ggtcccggat tcggcggcga gttcgaccat
     1501 ctccagcgcg gccgtggcgt tggccagcgc catcggcgcc gattgaccca attggcgcgg
     1561 gccgaaggcg acctcgtcgc ctaccgtccg ttcgacgatc tgatcatcgg gattctggaa
     1621 gacgaatccg acgcgggcgg ccatccgggc cacgccatgt tcgcgcgtgt cccaatcacc
     1681 gaccacgacc cggccgcgcg ccggcttcaa caggccgttg atatgcttaa ccagcgtcgt
     1741 cttgcccgcg ccgttctcgc cgacgatcgc cacagcctct cccgagcgga tgttgataga
     1801 gacgccgcgc agtgcttcga ctccgccggg ataggtgaag gcgatatcct caatcgacag
     1861 tttcatcgga agaactccac agcctgctcc agcgtgacgg ggagggtggt ctcatcggtg
     1921 cgccaaccgg cggcctgggc ggcgcgggcg gcctgtgtgt agcgcgtcgg ctccagccca
     1981 tattttgtca gtgtcagaga gctgagaact tcagccggcg gcccgtcggc caccagtttg
     2041 cctttatgga gcaccagcac ccgatcggcg aacgtcgcca cccattcgag cttctgctcc
     2101 gccaggacga cggtcgcttc gtcggtcgcc gccagatcgc gcaggacggc gaagacctct
     2161 ttcgagccga tgggatcgag ctgagaggtg ggttcgtcga ggacgaggat cttcggccgc
     2221 atcaccagca ccgaagcgat ggccaaccgt tgttgctgac cgcccgagag cgcgtagggg
     2281 gactggtcgg ccagatccgt caggcggaca aaggccagta cttcgtcaat tcgcggctcc
     2341 atctcggcca agggcacgcc caggttctcc agcccgaagg ccacttcttc gcggacggtg
     2401 aagcgcgcgc cggtgatctg gttgaacggg ttggcgaata ccaggcccac cgtgcccgcg
     2461 agttcagcca gcgatgaacc ggcggtatcg atgcccgcta cttcgacgct gcctgtcagc
     2521 ttgccgcgat agaaatgggg cacaaagccg gccagcgccg agcaaagcgt ggatttgccg
     2581 gaaccgttgg ccccgatgac ggcgcagaac tgccctgccg ggatttccag cgagagattg
     2641 ctcagagccg gggagtccga gcgcgggtaa tgataggtca cgtcgctcag actaatcacg
     2701 cggcaaacct cggagcaagc cacaaataaa tgcgcagggc gatgacgccc agcgagacca
     2761 aaatcaataa ccggcggaag aggcgttgtc ggttggtatc gtgcaaaacg agcagactcg
     2821 tcttcggccc gctccggccg aacgcccgca cttcgagggc gatggctcgt tcctcaatat
     2881 cgacgatgct gccaagcaat aggggaaaaa ccagaggcag gagcgcgcgc gtccttatca
     2941 ggaagctgcc ttccgtctca aggccacggc tgcgttgagc atcaaggatc gtgttggcct
     3001 tatgctggaa tcgcgggatg atctgcaacg tgctcagaat gacatacgag attcgactcg
     3061 gcaaaccctt ctgactgagg gccagcatca actcatccga ccgggtcgca taactcaaca
     3121 ataaaaagcc gctgacggcg atgataattc ggccggtgcc gcggatggca aacaatagcc
     3181 cctcttcctt cagggaaaag gggccaagtt gttggatgat ggtgccgcct gtccagaaca
     3241 acccctgtac gatgaacaag gatattgcga acgggatagt cgtttttatt gtgttttgga
     3301 caacgttggg gaatacctgc gccatcaggg caaccggaac gatcccgacc agcaacacga
     3361 tatacggcgc ccacacaccg ggcactatcc aggcgatgag caggcatgtc aggacataag
     3421 tgagcttggt caggggatgg agccggtgta ttgccgtttg tttgcttaca tacaaaccat
     3481 acgttgcact catggcactc cccggaactc tatctagcga atatcatcgg ctcttgagaa
     3541 gcggtcgata tatctgtccg gaagcgtgcg gatgatcagg taaacgacaa ggaaagagat
     3601 ggctttgtcc aacgggtcgg tggccagtcc ttgcagaaag ttcgcggcca gcatgctcat
     3661 gccctgactg cggaatgcgg cggtgatcac atccatcccg gtgccgacaa cgccgccgaa
     3721 aaggtagaaa gtgatgggag cggatagcaa cgacgctacg acgccggtta tcgcgccgcc
     3781 cagaacggcc ttccaccatg acttgaacca gccatacttg ccgaatacgc cggccaggaa
     3841 accaatcgcg gccgccaccg gcgcatatgg aacggtattg gttacgtcac cgaagatgaa
     3901 cgcccagacg atgttggaca gcaggccggc cacggcgccg acccacggcc cggccacggc
     3961 gccaacgaaa accgtgccga ttgagtccaa atacaatggg attctgagag ctttaacaat
     4021 ttgccctacg gcgatgttta tggcgatcgc gatagggatc atggccaatg ctatggtatt
     4081 aaagtcgcgt ttgagttttt ccatttttct ccatcctcct gccttgggaa tcaccctttc
     4141 gggcttgccc ggtagacgat aagacaccct gtatgaatca ttggcccctc tttcgagggc
     4201 catttttagg cccacttttg gacccatgtc tttgtgacca acattgtacg aaactttcaa
     4261 cttatgtcca gaacaggcat aggcaatttt cgatcgaaga gcatcaggcg tttgacgcgg
     4321 ggatgtggcg cccggcgtat ttagatcgca tggcgagata attggcttgg ctattatttg
     4381 gcgacaacgt cccaatttcg tatcatgtaa ggggatgtga aaggagcgtg aatctgccgt
     4441 aaggtttcct gtaagtcgct gcagctataa tagctgcgtc aactgtatcc tgatcgnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttg gaggcaagct ttgtttagga
     4621 agaactctct gaagcgcagt attaaactgt tggtcgtgct gggcatcctg gccctcacgg
     4681 cctccgtcgc tttcgcccag gatcgtccct ggaccacgca gggcgagcgc ggcaattact
     4741 ccgaacgtcc catgcccgcc cagatttcgg gcgcgaaact ggatactccg gctcaaatgc
     4801 aggttgagaa tctgtccgac ctgcgggtga accccgcatt gcgtggccag gccggccgcg
     4861 tgcaggttgt tgttcgtctg gccggtgcgt ccgccacgaa cggccccacg ctgcaagccg
     4921 cggtctcgca gcaaggcgct tttgtctcgg ccgcgcgcgc ggtcgattcg cgggccaagg
     4981 tccttggctc gacgcaggtc gtcatgaacg cggtcatgct cgaagttgac gcttccgctc
     5041 tgagcgccct ggcccagaac ccggacgtcg tctcgatcaa cccggttcgc aattacgagc
     5101 tggacctgtc cgagaccgtc ccctatatcg gcgcgacggc cgttcaggct gcgggcttcg
     5161 acggcacggg cgttcgcgtg gccgtgatcg acagcggtat cgactacacc cacatcgcct
     5221 ttggcggttc cggcgatccc aatgactggc tgaataacga cccgacgatc atcgagccgg
     5281 gaaccttccc gacggctcgc gtcgtgggcg gttatgactt cgtcggcagc gaatggccca
     5341 atggccccga gatgcccgat cccgacccga tcgatgatgg tccgggcggc ggtcatggca
     5401 cgcacgtggc cgacatcatc ggcggcgcga tgggcgtggc cccgggcgtt gatctctacg
     5461 ccctgaaggt ctgctcctcc gtcagctcct cctgcgccgg tgtgccgctg atccaggcga
     5521 tggagtttgc ggtcgacccc aacggcgacg gcaatcccga cgacgcggtt gacgtcatca
     5581 acatgtccct gggttcgtcc tacggcgatc cgcgttggga cgacctctcc tacgcggtca
     5641 acaacgccag cgccctgggt gttctaaccg ttgcctcggc cggcaacagc gccgacaagc
     5701 cctatgccaa cggcaccccc agctcggctt cgaccgcttt ggccgtggcc cagacgaacg
     5761 tgccttcggc gaaccaaccc atgatggaag tcactgcgcc ggcgagcatc gccgggctgt
     5821 atgcggccgt cttccagccg tgggcggtag agccgactgc gcgcatcgaa ggcccgttgc
     5881 agtatggcga cggcgcgggc ggcaacctga acggttgcac gtcgttcgcg cccggcacgc
     5941 tgaccggcaa gatcgtgctg gtcgatcgcg gcacatgcgg gttcagcctc aagatcagca
     6001 atatcgctga cggcggcgct ttggtcggca tcatcggcct catcgccccc ggagacccgt
     6061 tccagggtgg cttcggcgag ggcgacccgt cgatccccgg ctacatgatc agccaggctg
     6121 attccaacgc gctgaagagc gggttggcgg aaggcgtgac ggtcgtcttt gatccggcga
     6181 acggcatctc cctggtccag cacctggttg gctccagcag ccgcggcccg acggtcggca
     6241 cgaatgaggc cggccccgac atcggcgcgc cgggcgcgtc ggtatcggcc gtttggggat
     6301 ccggcaccgg caccgaagcc ttcggcggca cctccggcgc cgcgccgatg gtcaccggtt
     6361 cggccgccct gctcatgagc gcctacccgg atcgcacgtg gtcggagatc aaggctgttc
     6421 tcatgaacac cggcgagacg gacatgatga acaatcctgc gttcttcggt gggtatcagg
     6481 ccgcgatcag ccgcatcggc ggtggcgagg tgcgtgtctt cgatgcgctc aactcgaccg
     6541 tcgccgcatg ggaccgcaat gccctggatg ccacgctgtc ctttggcttc cacgatatcg
     6601 ttggcaacca gaccatggtg aagcgtgtca cggttcgcaa ctacagcaac cagcctgtca
     6661 cgctccgttc caatgttgtg tatcgttatg acaatgatga taccggtgtg gttcgtctct
     6721 ctgtcccgtc gtcgttcacg gttccccgaa acggcagtgt gaccttcccg gtccacatgc
     6781 acattcgccc gcgcaccaac cagccgctgc acgactgggt gctgaacagc gacagcaacg
     6841 gggcaaaccc cgacgcgctg accctcaacg aatatgatgg ttacatcaat ttcgagggtg
     6901 tcggcgggag caccgcaagc atccacatgc cctggcacgt gctgccgcgt ggcgcgggca
     6961 acatccagtc gggcgttcgt cagaacggtt tgaactgggt ccgcaacacg ggcgtcgctg
     7021 cgtcctacat cgacacctac tcgctgatcg gcatgagcga ccagcagccc gacgcgcccg
     7081 tcctgggtgg caacgcggct ccggccgacc tgcgttatgt gggtgtgcag acctacccgg
     7141 ttccggccgg gtactgcggc gccgagccgt cgtttgtcat ggccttcgcg gtcaacacct
     7201 gggatcgtta ttcccatgcc gccgccaata tctggattga ggccgacgtg gatgtggatg
     7261 gcgacggtct ggccgactac gcgatcttca atttcgacaa ttccggtggc ttcagcagcg
     7321 ctccttctga cggccgtgcc gtgacctggg tctatgatct gagcaccggt tccgccgatg
     7381 cgtggttcta cgcgacccac gagaccaaca gcggcaacgt tgtgttgact ttctgtggcg
     7441 atcagatcgg cctgaacgcg aatgacttct tcacgacgtc gatgggtgtc gacgtcttcg
     7501 ccaatgactg gtactacagc ggcggcgcag atgctatcac cggcctgaac gtcgttccgc
     7561 tcggcgagcg ctactacacc atcttcggca atggcgacgc gggtttaacc gagttgccgg
     7621 cgcgctccgg cgcgctgccg ttcggtgtga tcgacttcgg cgatcagatg aacgcgaccg
     7681 agaacggctt gctgtggatc tatggtcccg gtgccccggc ggacaacgag gcccaggcct
     7741 ggatcatcaa ctaatatcgg ttccccaagc cccggtttcc tcgcgggaat cggggcttgt
     7801 ggggtaagaa aatcaatcgc tccgtagtct gattggtaga acaaacagag aaacaagcgg
     7861 ttctcatttt cttcctcctt agctaaaacg ccgcacccgg ttttcgggcg cggcgttttt
     7921 gcttctcctg cttggggtta tgtgcctaac ccaatgcctg atcgatatcg gcgatgatgt
     7981 cgtcgatgtg ttccaggccg acggagacgc gaaccagacc gggcgtgatg cccgtcgcca
     8041 accggtcttc ctcgctcagg ctgctatggg tcgtggaagc cgggtgcgtc accgttgtgc
     8101 gcgcgtcgcc gaggttggcg acgatgtcgc acagcctgag cgcgtccagg aagcgtcgcc
     8161 ccgccgcgag cccgccggcc acggtaaatg tcaccaggcc accccccgcc gacatctgtc
     8221 gccgcgcgag atccacctgc ggatgtgagg ccaggaacgg gtagcgaacg gattcgacac
     8281 ccgcgcgccc ttcgagccag ttggccaagg tcaacgcgtt ggcgcaatgg cgctccatgc
     8341 gcaacggcaa ggtctccaaa gcctggctca caatccagct gttgaaaggt gaggccgtcg
     8401 gccccgtttg gcgggcgaat tgggtcagtt cccgcatgat gaatgagtcg cccagtatca
     8461 cgccgccgat agtgcgcccc tgcccatcga ggaatttcgt cgtcgaatgg atgaccagat
     8521 ctgctcccag ttcgatgggc cgctggacga tcggcgtggc gaaggtatta tcgacgacca
     8581 gatagacgtc gcgctcccgg cacatggccg cgacctgggc gatgtcgacc agcgtcaggc
     8641 cggggttcga cggcgtctca atcaggcaga tgcgcgtctc cggccgaaaa agnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgttggg gttgccccaa cgggagtagg
     8821 cgtagccggg cttttcgccg ctgaagaccg cgcgcgccgt ttcggcatcg ggaaaggcaa
     8881 agccggaagc caggtagatc ggcgtgatct gctcgcgatg ggggccgatg gcggttcgct
     8941 cgcgaatcag gcgggtttca atatgggcgg attttaaatc agtcatgaag tcacctcgtg
     9001 gtgtaagtcg atggaatatc cgggttttat atctttttac cactccggcg gcgaatgtcc
     9061 gtcatttgcc cttcgcatcg ggccaacatg gactgatttc actaaatcat catgcaatcg
     9121 tcgggttaga aggcttgtca ccctctcata atctcgctat agtgcccatg atggaagcga
     9181 ccgtcccact atcggccgcc tggcgaacgg atcctggcct ggtgcgaagt cacaatgagg
     9241 actttgtcat cgtccatgag ccggagtcgc cggacgacta ccaattgcat ggcgcattgt
     9301 atatcgtcgc cgacggtgtc ggcggcgctg acgcgggcga ggtcgccagc cagtacgcca
     9361 gcgagcagac actccaccat taccttcagc cgatccaatt cgatacgctc ggcgcgcgac
     9421 tgatcgaggc catgcagtcg gccaatacgg atctgcgacg cctggcggcc gagcgcgacg
     9481 acagccgccg catggcaacg accatggtgg ccgtggccgt tcaggatggc catgcctaca
     9541 tcggcaacgt cggcgacagt cgcgcctatc tttggcgtcg cggggtgatc agccaggtga
     9601 cgcgcgatca gagccttgtc gcgcgattgg tggaagaggg cgcgctgacg gctgaagaag
     9661 cgctccgtta cccatacaag aacgtcattc tctacagttt gggatcggaa aggcggcctc
     9721 ccatcgattt gtttgaggta gagcttgagc ttggcgatct tctattgctc tgttccgatg
     9781 gcctgacgcg ccatgtcccc gatgaagaga ttgcgcgcat cattgccggc gaagaactcg
     9841 acgcggccgt cgagacgatg gttcgcctgg cgcggcagcg agggggcgag gacaacatca
     9901 cggtggccat cgtacaacat ggcccgcgcg aggcggcatt gccggaaata gatgccgagg
     9961 ccgcaatgct gacgattgcc cggccacgtc ctgatgtgca aactgctatt cgcgccttgc
    10021 ctgtggcggc cgaaccccat ccgcgcatgt ggccgcttct tctcgtgctg acgaccgcca
    10081 tggttgtgat gatcttcctg ctgtggctat tcattcaacg cgtaaccaca atttagcccg
    10141 cgccttatgc gacttcgtga gcgtttttga cgcgagtggt aaatcctcat aaaatttcct
    10201 gtagtatgac aaacgcatta aagatattac ccctaggagg gc
//
LOCUS       DAOMQG010000096        10158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2849_length_10158_cov_11.770365, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000096 DAOMQG010000000
VERSION     DAOMQG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_10158_cov_11.770365"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLC06_07205"
     CDS             <1..888
                     /locus_tag="PLC06_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRO90270.1"
                     /translation="EVGTTKPAADSARIIIAEVNRQMPRTLGDSFIHVSRLSWVVETD
                     YPLPEAPQGGSSEATLQIGQHIAEMIPNGATLQMGIGSIPDAVLQNLHTHKDLGIHTE
                     LFSDGVVSLFEQGVINCSRKSFHPGKMIAGFLFGSGKLYHFVHNNPIVELHPTEYVND
                     PFNIAQNNNMVAINAALQVDLTGQVCADSIGPVLYSGVGGQADFIRGAARSKGGLPII
                     ALPATAKNDTISRISPLLTEGAGVTTTRNDVHYVVTEFGVASLYGKTIRQRSQALINI
                     AHPDFRDELTHAAKKLGYL"
     gene            complement(1012..1926)
                     /gene="focA"
                     /locus_tag="PLC06_07210"
     CDS             complement(1012..1926)
                     /gene="focA"
                     /locus_tag="PLC06_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HRO90271.1"
                     /translation="MNQNPPLPSLDALLPAEMAKKAENVGVTKANLGPFRMFALAVLA
                     GAFIAMGAVFMTIVTTGATGILSYGVTRLLAGLAFCLGLILVVVAGAELFTGNNLIIM
                     AWAGGKISTGKLWRNWIIVYLGNFAGSILTAYGMFLSGQYLFNKGAVGLNALNIAAAK
                     TSLDFIPALVLGVFCNALVCLAVWLCMSARTTVDKIVAIIFPISAFVAAGFEHSVANM
                     YFIPVGLFIKNGAPAEFWAAIGQTSADFASITWSNFLIANLLPVTLGNIIGGALMVGL
                     VYWFIYLRPSWTGRKDTAEEPDKKLKST"
     gene            2158..2334
                     /locus_tag="PLC06_07215"
     CDS             2158..2334
                     /locus_tag="PLC06_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90272.1"
                     /translation="MDQDVELKETRIYPDSDFLRIVGDEYRVRMCACTAAIECNLAGI
                     PCQWALNSPDNDRF"
     gene            complement(2472..>3158)
                     /locus_tag="PLC06_07220"
     CDS             complement(2472..>3158)
                     /locus_tag="PLC06_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRO90273.1"
                     /translation="ASLNGVSSGGWIHYAKEMQGAGADAIELNIYYLPTRIDLTGAEV
                     EKMYVDLVRDVRASIDIPIAVKLSPFFSSTANMMHQLSEVGANGLVLFNRFYQPDLDL
                     ENLEVVPNLVLSRSEEMRLPLRWIALLYGRISADLALTTGVHGADDVLKAVAAGANVA
                     MMTSEILLNGIGRFSEILAEMKLWLEEREYISIRELHGSLSQVNVAAPTAFERANYIQ
                     IVKSYAPTFR"
     assembly_gap    3159..3258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3417..7073)
                     /gene="nifJ"
                     /locus_tag="PLC06_07225"
     CDS             complement(3417..7073)
                     /gene="nifJ"
                     /locus_tag="PLC06_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRO90274.1"
                     /translation="MTTKMITIDANEATAGIAHKVNDVIAIYPITPSSGMGELADQYS
                     AHGDTNIWGAVPLVIEMQSEGGAAGAVHGSLQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTTFHVAARSLAAQGLSIFGDHSDVMATRNTGFALLCSNSVQEAMDFALIAHSAT
                     LKTRVPFIHFFDGFRTSHEINKITPINEAAMRAMINDDLVKAHRDRALSPDKPIMRGT
                     AQNPDVYFQARETVNPFYEACPGLVQATMDELANLTGRQYHLFDYVGAPDAERVIVMM
                     GSGAEAAEEAVHYLNQHGEKLGLVKVRLYRPFDTAAFVKALPLTTRSIAVLDRTKEPG
                     STGEPLYLDVVSALVERVADGSAPFAVMPRIIGGRYGLSSKEFTPAMVKAIYEELAKP
                     VMKNHFTIGINDDVTHTSLAYDETFSTEPDDVVRAIFFGLGSDGTVGANKNSIKIIGE
                     NTPNYAQGYFVLDSKKSGSVTVSHLRFGPRPIHSTYLITNANFVACHQFGFLERFDML
                     KLAQPGATFLLNSPYGPDEVWDHLPQETQRAIIEKDLHFYVVDAYAVAEETNMGRRIN
                     TVMQTCFFALIERATSAPLLSRERAIAEIKDAIYKTYGKRGETVVQQNYAAVDATLSH
                     LHEVQVPGEVSSRFTRPAVVPNHAPDFIHSVTAPMMAGLGDMLPVSALPVDGTYPTGT
                     TQWEKRNIATEVPLWDSDICIQCGKCAFVCPHAVIRLKIYDPIHLEDAPEGFMSTPAR
                     FKEFKDLSYTLQISPEDCTGCSLCYEVCPVKNKRQPKFKAINMVDYTPEVRATESANW
                     DFFQTLPNVERTDIPFNQVKYNQLLEPLFEFSGACAGCGETPYLKLITQMFGDRMLVA
                     NATGCSSIYGGNLPTTPWSKNAEGRGPAWSNSLFEDNAEFGLGMRVALDQRLDAVTNT
                     LRTLRELIGAQLTDEVLYSDQITEMDILSQRRRVQLLRERIQHLLNSHDDSIVPVKPA
                     LADLMSKIDVLVKKSVWIVGGDGWAYDIGYGGLDHVIASGRNVNILVLDTEVYSNTGG
                     QMSKSTPRGAVAKFAAAGKPLPKKDLGLLAMSYGNVYVARIAFGANDRQTVQAIMEAE
                     AYDGPSLIIAYSHCIAHGYDLKYGLDQQQAAVDSGHWTLYRYNPDLAIAKSDGHESTI
                     SHNPFQLDSKAPTLPLEEYIYREGRYRMLQQSNPEAAERLLELAKLDVAERWESYRKM
                     AMSHEKTERTMAGD"
     gene            complement(7152..9869)
                     /gene="ppdK"
                     /locus_tag="PLC06_07230"
     CDS             complement(7152..9869)
                     /gene="ppdK"
                     /locus_tag="PLC06_07230"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRO90275.1"
                     /translation="MTKWVYLFNEVDEAEKHMGGHWDGVRSLLGGKGANLGDMTRLGI
                     PVPPGFTVSTEACNAYLESEGFPAGMWEQELVAIQEIEKLTGKKFGGADNPLLVSCRS
                     GAKFSMPGMMDTVLNIGLNDEIVETMVRLTGDRRFVFDLYRRLIQMFGSVVMGVPDEA
                     FEAVITARRKLAGVTSDSELKAADWEVVTRRFKEIYRTFTRSDFPTDPHEQLRLATEA
                     VFKSWNGRRAIDYRNAAGIAHDLGTAVNIQTMVYGNIGDNSATGVAMSRNASTGENEP
                     EGDFLVNAQGEDVVAGIRLTQPLHELKAIMPVAYNEFLTIARKLEKHYRNMQDMEFTI
                     EDGKLWLLQTRDGKRTAQAEVKIAVDMVEEGLISKEEAVWRVKPAQVDFFLHPQLDSA
                     ALKSARKIAKGLNVSPGAAVGMVAFDADTAERWVKDEGRKVIMVRPETKPDDVHGMLA
                     AQGILTSRGGRTSHAALVARQFGKPAVVGVMELEIDLDSREMAIGEDIIVKEGDWISV
                     DGTLGIVYLGQVPTVVPDIKNPYLIKLLSWADEFRAIGVWANADYPQDARRAREYGAQ
                     GIGLCRTEHMFFEAARLPIVQQMIMAKNVTERNEALDKLLPMQRGDFDGLFRAMDGQP
                     VIIRLIDPPLHEFLPSYEELVLRLADLKVQLQHFHTLSEIDEALSEIRIKQAYLERVE
                     GLREQNPMLGTRGVRLGILIPELTKMQVRAIFEAACQCQKDGIDVHPEVMIPLTFHIN
                     ELKVQQTTLEAVAKEVMDEQGIQITYKFGTMIEIPRAALTADEIAEIAQFFSFGTNDL
                     TQTTFGISRDDAEAGFLVEYQQRNILEENPFGTLDVPGVGKLMKMAVELGRATRPDLE
                     IGICGEHGGDPRTIAFCHQIGLNYVSCSPFRVPIARLAAAHAALADKKQ"
BASE COUNT         2123 a         2766 c         2897 g         2272 t
ORIGIN      
        1 gaggtgggca cgaccaagcc cgcggccgat tcagcgcgta tcatcatcgc cgaggtgaat
       61 cgacagatgc cgcggacact gggtgacagc ttcatccacg tcagccgatt gtcgtgggtt
      121 gtggagacgg attatcctct gccggaagcg ccgcaaggtg gcagctcgga ggctaccctg
      181 caaatcgggc aacatatcgc cgagatgatc cccaacgggg caacgcttca aatgggtatc
      241 ggcagtattc ccgacgcggt attgcaaaac cttcacactc ataaagacct gggcattcac
      301 accgaactgt tctcggatgg tgtggtttct ctgtttgagc agggggttat caattgcagc
      361 cgcaagtctt tccatccagg caagatgatc gccggtttcc tgtttggctc cggtaagctc
      421 tatcattttg tccataacaa cccgattgtc gaacttcatc caacggaata cgtcaacgac
      481 ccctttaata tcgcccaaaa taacaacatg gtagcgatta atgccgcctt gcaggttgat
      541 ttgaccggcc aagtgtgcgc cgattctatt ggcccggtcc tctatagtgg ggtgggcggt
      601 caggcagact tcattcgtgg cgcagcgcgc tcgaagggcg gcttgccgat catcgcgctc
      661 ccggcgacgg cgaagaacga cacgatcagt cgaatttcac cgttgctgac cgagggggcg
      721 ggcgtcacga caacccgcaa cgatgttcac tatgtcgtga ctgaatttgg agtagcttct
      781 ttgtatggca agaccattcg tcaacgatcc caggcattga tcaatatcgc tcatcctgat
      841 ttccgcgacg agcttaccca cgcagcgaag aagcttggct atctatagac gtttgtgatt
      901 ctccttcatc taaacgggca acccccttca gcctcaatgc tggagggggt tgttcgttta
      961 ttgggatggg tgagtccggg tgatgtacag cgaggtacct tgcccccagg ttcaggtact
     1021 tttcaacttt ttatccggct cttcggcggt atccttccgg ccggtccagc ttggtcgcaa
     1081 gtagataaac cagtacacga ggccgaccat cagtgctccg ccgatgatgt tccccagcgt
     1141 caccggcaac aggttggcta tcaggaaatt gctccatgtg attgaagcga aatcggccga
     1201 ggtctggccg atggctgccc agaattccgc cggcgcgccg ttcttgatga acaggcctac
     1261 ggggatgaag tacatgttgg ccacgctgtg ttcgaatccg gcagccacaa aagcgctgat
     1321 tgggaagatg atcgccacga tcttgtccac tgtcgttctt gcgctcatgc acaaccagac
     1381 ggccagacac acgagggcat tgcagaagac gcccagcacc agagcgggaa tgaaatcaag
     1441 gctcgtcttg gcggcggcaa tattcagcgc atttaatcca accgcgcctt tgttaaacaa
     1501 atactgacca cttagaaaca taccgtatgc ggtcagaatc gagccggcga aattgcccag
     1561 atagacgatg atccagtttc gccataattt accggtactt atcttgccac cggcccaggc
     1621 catgatgatc agattattgc cggtaaacaa ttcagccccg gcgaccacga cgaggatgag
     1681 accgagacaa aaggcaaggc ccgccagaag tcgtgtgacc ccatagctga gtattccggt
     1741 cgcgcctgtg gttacaatgg tcatgaagac ggcgcccata gcaatgaacg cgccggctag
     1801 aaccgccagc gcgaacatgc gaaatggccc cagattcgcc ttggtcacgc cgacgttttc
     1861 ggcctttttg gccatttctg cgggtaataa agcatcaagt gagggcaatg gtgggttctg
     1921 attcatttgg aagactcctt tgccccatat cataaatgaa actgggttgt acttattagt
     1981 gatatatttc acaaggaagg atgacaaatg tcatctcatc cgatgacgat tgacactaaa
     2041 aggatcgatg aggttttgat atgatgctac caatgacgag atcgtcggga cagcataagg
     2101 atggatggaa tggccataaa gacagtggta actatccggt caaaacattg tgagatattg
     2161 gaccaggacg ttgaattaaa agaaacccgc atttatcctg attccgactt tcttcgtatc
     2221 gtgggcgatg agtatcgcgt ccgcatgtgt gcttgcacgg ccgccatcga gtgtaacctc
     2281 gccggcattc cgtgtcagtg ggcgctgaac agtcccgaca acgaccgctt ctaatgaatt
     2341 gacggattca ttacttccgc tacagtcggc tttccgacaa cactacctcc gacggaagag
     2401 gcccgagaga aatcccgggc ctcttctgca ttgatccgct atgtgtcaag cgaaagtact
     2461 gattgttgga atcaacgaaa tgtcggcgcg taggatttca cgatctggat gtagtttgcg
     2521 cgctcgaaag cggtcggggc ggcgacattg acctggctca ggctgccgtg tagttcacgt
     2581 atcgagatat actcgcgctc ttcaagccac agcttcatct cggccaggat ttcggagaag
     2641 cggccgatgc cgttgagcag gatctccgat gtcatcatcg ctacattggc tccggcggca
     2701 actgctttca acacatcatc ggcgccatgg acgccggtag tgagcgccag atcggccgag
     2761 attcgcccat agaggagggc gatccagcgc agcggaaggc gcatctcctc tgatcggctc
     2821 agcacaagat tcggcacaac ttccaggttt tcgagatcga gatcgggctg atagaaacga
     2881 ttaaaaagga ccagaccgtt tgctcccact tccgagagct gatgcatcat attggccgtg
     2941 gagctgaaaa aagggcttag cttgacggca ataggaatat cgatcgaagc ccgcacatca
     3001 cgcaccagat cgacgtacat cttttcgact tcagcgccgg ttaagtcaat cctcgtcggc
     3061 aggtagtaga tattgagttc aatcgcatcg gcgccggctc cctgcatctc tttggcataa
     3121 tggatccatc cgccggaaga gacgccgttg aggctggcnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnga ggctgacgcg accaacgggt tcttcaaatc aaaccccagg
     3301 tatgttgttc tcaggtccac gcttcactct cctgaatgct gattagctgg tgaccgtgca
     3361 gattaaatgg ctttataact cgatataatc cgcacggccc acagccattt ttgtcgttag
     3421 tcgccggcca ttgttcgctc ggtcttctca tggctcatgg ccatcttcct gtagctttcc
     3481 caacgctcgg ctacgtcgag tttagccagt tccagcaatc gctcggcggc ttccgggttg
     3541 ctctgctgca acatccgata gcggccctcg cgatagatgt attcttccag cggcagcgtc
     3601 ggcgccttgg agtcgagctg gaacgggtta tggctgatcg ttgactcatg gccgtctgat
     3661 ttggcaatgg ccagatcggg gttgtagcga tacagtgtcc agtggcccga atcgacggcc
     3721 gcctgctgct gatccagacc atacttcaga tcgtagccat gggcaatgca gtggctgtag
     3781 gcgatgatga gcgatggccc atcgtaggct tccgcttcca tgattgcctg aactgtctgg
     3841 cggtcgttcg ctccgaaggc gatacgggcg acgtagacat tgccatagga cattgccaga
     3901 aggccgaggt ccttcttggg caacggcttg cccgcggccg cgaacttggc gacagcacca
     3961 cgcggtgtag atttcgacat ctggccgccg gtattggaat atacctcagt gtccagaacc
     4021 aggatgttga cgtttcggcc ggaagcgatg acgtggtcga ggccaccata gccgatatcg
     4081 taagcccatc catcaccgcc gacgatccac acgctctttt tcaccagcac gtcgatcttg
     4141 ctcatcagat ccgccagcgc cggtttgacc ggcacgatcg agtcgtcgtg gctgttcagc
     4201 agatgctgga ttcgctcgcg gagaagctgt acccgtcgtc gttggctcag gatgtccatc
     4261 tcggtgatct ggtcgctgta gagaacctcg tctgtgagtt gcgcgccgat cagttcccgg
     4321 agtgtacgca gcgtgttcgt gaccgcatca agtcgctggt cgagcgctac gcgcatgccc
     4381 agaccaaact cggcattgtc ctcgaacagg gagtttgacc acgccgggcc gcgcccctcc
     4441 gcattcttcg accacggggt tgtcggcaga ttgccgccgt agatcgagga gcagcctgtc
     4501 gcattggcga ccagcatccg atcgccgaac atttgggtga ttagtttgag atagggcgtt
     4561 tcgccgcatc cggcacatgc ccccgagaac tcaaacagcg gctccagtaa ctgattgtac
     4621 ttgacctgat tgaacgggat gtcggtgcgc tccacgttcg gtagcgtctg gaagaagtcc
     4681 cagttcgctg attctgtggc ccggacttcc ggggtgtagt ctaccatgtt gatggctttg
     4741 aactttggct gccgcttgtt cttgaccggg cagacttcgt agcataacga gcacccggtg
     4801 cagtcttcgg gcgaaatctg caaggtgtag gatagatcct tgaactcctt aaagcgcgca
     4861 ggagtggaca tgaagccttc cggcgcgtcc tcgagatgga tcggatcgta tatcttcaga
     4921 cgaataaccg catgcggaca gacaaacgca cacttgccgc attggatgca gatgtcggaa
     4981 tcccacaacg ggacttcggt ggcgatgttg cgcttttccc attgcgttgt gccggtcgga
     5041 taggtgccgt ctacgggcag ggcgctcacg ggcagcatat cgcccagccc cgccatcatc
     5101 ggggccgtga cggaatggat gaagtccggc gcgtggttgg gaacaacggc cggccgcgtg
     5161 aagcgactgc tgacctcgcc tggtacctgc acctcatgca aatgagacag cgtcgcgtcg
     5221 acggcagcat agttttgctg cacgacagtc tcgccccgct tgccgtaggt tttgtaaatg
     5281 gcgtccttga tctcggcgat cgctcgctcg cgcgacagaa gcggcgccga ggtcgcccgt
     5341 tcgatcaggg cgaagaagca ggtctgcatc acggtgttga tgcggcgacc catgttcgtc
     5401 tcctcggcaa cggcgtaggc atcgactacg tagaagtgca agtccttctc gatgatggcc
     5461 ctctgagttt cttgtggcag atgatcccag acttcgtcgg ggccataggg gctgttcaac
     5521 agaaatgtgg cgccgggttg ggccagcttg agcatgtcga accgttcgag gaatccaaat
     5581 tgatgacagg cgacgaagtt agcatttgtg atcagatagg tggaatggat cggccgtggc
     5641 ccgaagcgca aatgggacac agtgaccgaa ccggacttct tcgagtcgag cacgaagtaa
     5701 ccctgtgcgt agttcggcgt gttctcgcca atgatcttga tcgagttttt gttcgcccct
     5761 accgttccat cggaacccag tccaaagaag atggcgcgaa cgacatcatc cggctcggtg
     5821 gagaaggtct catcataggc cagactggtg tgagtgacgt catcgttgat gccgatcgtg
     5881 aagtggtttt tcatgaccgg cttggccagc tcttcataga tggccttcac cattgccggt
     5941 gtgaactcct tggaggagag gccataacgg ccgccgatga tcctcggcat gaccgcaaat
     6001 ggcgcactgc cgtcagccac gcgctccacc agcgccgaga cgacgtcgag atagagcggc
     6061 tcgcccgttg agccgggttc cttcgtgcga tccaggacgg cgatgctgcg tgtcgtgagc
     6121 gggagggctt tgacgaaggc ggccgtgtcg aacggtcgat agagacgaac cttgaccaac
     6181 ccgagctttt cgccgtgctg attcaggtaa tgaaccgctt cttcggcagc ttccgcgcct
     6241 gaccccatca tgacgatgac acgttcggca tcgggagcac cgacgtaatc aaacagatgg
     6301 tattgacgac cggtcagatt ggccagctca tccatcgtgg cctggaccag gccgggacaa
     6361 gcctcataaa acgggttgac ggtttctcgc gcctggaagt agacgtcggg gttttgtgcc
     6421 gtgccacgca tgatgggttt gtcgggagaa agcgcgcgat cgcgatgcgc cttcaccaga
     6481 tcgtcattga tcatcgcccg catggcagct tcgttgattg gggtgatttt gttgatttcg
     6541 tgcgaggtgc ggaaaccatc gaagaagtgg atgaacggca ctcgcgtctt cagggtggcc
     6601 gagtgtgcga tgagcgcgaa atccatcgcc tcctgaacgg agttggagca caggagggcg
     6661 aagcccgtat tacgggtcgc catcacatcg ctgtgatcac cgaaaatgga caggccctgg
     6721 gctgccagag agcgcgccgc gacatggaat gtcgtcgagg tcagttctcc ggcgatcttg
     6781 tacatgttgg ggatcatgag cagcaggccc tgcgaggcgg tgaacgttgt cgtcagcgct
     6841 cctgtttgca atgagccgtg cacggcgcct gccgcgccac cttccgattg catctcgatg
     6901 acgagcggca cggcccccca gatgttggta tcgccgtggg ccgaatattg atcagccagt
     6961 tcgcccatgc ccgatgacgg tgtgatcgga tagatggcga tcacgtcatt aactttatgc
     7021 gcgatgcccg cagtagcttc attcgcgtcg atggtgatca ttttcgtcgt catgataatt
     7081 ccttcacctc aatatttttg ttgtgccggc aagcaagcgc tggccggcac aacacggatt
     7141 gcatcgataa actactgctt cttgtcggcc aaagccgcat gggcggccgc cagacgggca
     7201 atcggcacac ggaatggaga gcaagagaca tagttgaggc caatctgatg gcagaaggcg
     7261 atagtcctcg gatcaccacc atgttcgccg cagatgccga tctcaaggtc gggtcgagtc
     7321 gcgcggccaa gctcgacagc cattttcatg agctttccga cgccgggcac gtcgagcgtg
     7381 ccgaacgggt tctcttcgag gatgttgcgt tgctggtatt cgaccaggaa gccggcctcc
     7441 gcgtcgtcgc gagatatgcc gaacgtcgtc tgtgtgaggt cgttcgtgcc gaaggagaag
     7501 aactgggcta tctcggcgat ctcatcggcc gtcagcgcgg cgcgtgggat ctcgatcatc
     7561 gtgccgaact tgtaagtgat ctggatgccc tgctcgtcca tcacttcctt ggctacagct
     7621 tcgagggtgg tctgctgcac cttcagttca ttgatatgga acgtgagcgg gatcatcact
     7681 tctggatgca catcaatgcc atccttctgg cattgacagg cggcctcaaa gattgcgcga
     7741 acctgcatct tggtgagttc ggggatgagg atgccgaggc gaacgccgcg ggttccgagc
     7801 atggggttct gctcgcgcag accttcgacg cgctccagat aggcttgctt gattcgaatt
     7861 tccgataacg cttcgtcgat ctcactcagc gtgtggaagt gttgcaactg gaccttcaga
     7921 tcggccaggc gcagcaccaa ctcttcgtac gagggcagga actcatgcaa tggcgggtcg
     7981 atgagacgga tgatgaccgg ttggccatcc atggcgcgga aaaggccgtc gaaatcaccg
     8041 cgctgcatcg gcagcagctt gtccagcgct tcgtttcgct ccgtcacgtt tttggccatg
     8101 atcatctgct ggacgatcgg caatcgtgcg gcctcgaaga acatgtgctc ggtgcggcag
     8161 agaccgattc cctgagcgcc gtattcgcgt gcgcgccgtg catcttgcgg gtagtctgcg
     8221 ttggcccata cgccgatagc gcgaaattca tcagcccagc tgaggagctt gatgaggtag
     8281 gggttcttga tgtccggcac gacggtcggg acctgaccga gatagacaat gcccagcgtt
     8341 ccatcgacgg aaatccagtc gccttctttc acgatgatgt cttcaccgat agccatctcg
     8401 cgcgaatcca ggtcgatctc caactccata acgccgacca cagccggttt gccaaactgg
     8461 cgggcgacca atgccgcgtg gctggtgcgg ccgccacggc tcgtcaggat accctgtgcc
     8521 gccaacatgc catggacgtc gtccggcttc gtttccgggc gaaccatgat gaccttgcgc
     8581 ccttcatcct tgacccagcg ctcggcggtg tcggcgtcaa aagcgaccat accgacggcc
     8641 gcgcccggcg agacgttcag acccttcgcg atcttgcgag ccgattttag tgctgcagaa
     8701 tcgagctgtg ggtggaggaa aaagtcaact tgcgccggtt taacgcgcca gactgcctcc
     8761 tccttgctga ttaatccttc ttcgaccata tcgacagcga tcttcacttc ggcctgagcg
     8821 gtacgcttgc cgtctcgggt ttgcaacaac cagagcttgc cgtcttcgat cgtgaactcc
     8881 atgtcctgca tgttgcggta atgcttctcc aacttccgcg cgatggtgag gaattcattg
     8941 taggcgaccg gcatgatcgc tttcagctca tggagcggtt gggtcaggcg aatgccggcg
     9001 acgacatctt cgccctgagc gttgaccaga aagtctcctt ccggttcgtt ttcgccggtg
     9061 gaagcgttgc gcgacatggc gacgccggtc gcggaattat cgccgatatt gccgtagacc
     9121 atcgtctgaa tgttgacagc cgtacccaga tcgtgagcga tgccggcggc gttgcgatag
     9181 tcaatggcgc ggcggccgtt ccatgatttg aacacggctt cggtggccag gcgcagctgc
     9241 tcgtgtggat cggttgggaa atcgctgcgg gtaaaggtgc gatagatttc cttgaagcga
     9301 cgggtgacga cttcccagtc ggccgctttt agttcagagt cggaggtgac cccggccagc
     9361 ttacgacgag ccgtgataac agcttcgaac gcctcgtccg gcacgcccat gacgacgctg
     9421 ccgaacatct ggatgagacg acgatacagg tcgaagacga aacggcggtc accggtgaga
     9481 cgcaccatcg tctcgacgat ttcgtcgttc aggccgatat tgaggacggt gtccatcata
     9541 ccgggcatcg agaacttggc gccggaacgg catgagacga gcaacgggtt gtccgcgccg
     9601 ccaaatttct tgccggtcag cttttcgatt tcctggatag caaccagctc ttgctcccac
     9661 attcctgcgg ggaacccctc ggattcgaga taggcattgc aggcttccgt cgagacagtg
     9721 aagcccgggg gaacgggaat acccaggcgg gtcatgtcgc caagattggc tcccttgcca
     9781 cccagcaagg aacggacacc atcccaatga ccgcccatat gcttttcagc ttcatcgact
     9841 tcgttaaaaa ggtagaccca tttggtcacg ttagtgggcc tgtgaccatt accattggtt
     9901 ttctgatgtt tcacaggtgc agacaaaaca ttttccgaca tgagtatgct ctccttcaag
     9961 atcatgtgcg gcgcatttga agacgatggc tgttattgcc tgataatgaa ttttcgtttc
    10021 aaatgttcaa gcggcacaaa aatataccgg tatcaattct taattagttt aaggatgaag
    10081 ccggattggg gttagtgaca gattgcacct gtcttgacga caaaagtcat accatgataa
    10141 cgtgcctgag aagatggc
//
LOCUS       DAOMQG010000097        10116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2865_length_10116_cov_12.008250, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000097 DAOMQG010000000
VERSION     DAOMQG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2865_length_10116_cov_12.008250"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..2037
                     /locus_tag="PLC06_07235"
     CDS             163..2037
                     /locus_tag="PLC06_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRO90276.1"
                     /translation="MFRTLRIFFTSSLVKGIVGQYVAWALGYGFIAMLRSFRGQPTPW
                     LFEPAIAFGALVSVLGFLLAAGVLTDWLKWIVGKRTPLAHGVPAGKPEWSRYFNVDYN
                     HKVIGIQYGVTSLLVLLVGGLFAIIFRIELAHPGMQWLTNDQFNTLFSAHGIVMIASI
                     LLGVGAMVNYLVPLMIGASDMAFPRLNAFSFWVTIPSVTLILTGMAVGGWDTGWVGYS
                     TLSLRAPLGIVLFLLGFWVNGFSSIASSLNMLVTATTMRAKGMTLFRMPIVVWGAAAA
                     SIIQLTATQTVGVALTLTVAERVLGLNFFDPSGGGNPILYQHLFWFYSHPVVYVFVLP
                     GLAVISELLPVFVRKPLFGYRWVALSSLGIGLVGFLVWAHHMFTSGMADALRMPFMFS
                     TMMVAVPTGVKFFSWVATMWEGKIPRNPPTPLLFVFGAISIFLIGGVTGPILGTIPTD
                     LHLHDSYWIVGHFHATMFGGYIFPFFAALYYWYPKISGRMYKESLGKLHFWLMLPAFW
                     VMSIGQMSVGLLGMRRRVADYDPALGITTTQLLVTLSALVIGWSVLIMIYNFISSARS
                     QTAADINPWRSRSPEWQIPSPVPEFNYSVPFEVVGEPYDYGLADSIYTRDLVPAPSGD
                     "
     gene            2059..2259
                     /locus_tag="PLC06_07240"
     CDS             2059..2259
                     /locus_tag="PLC06_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90277.1"
                     /translation="MNDKKAAAYRLGLITLVILAVLTIVEFIIAVYMGALVLLFIIAL
                     LKAAVIIQNFMHIARLWQEESH"
     gene            2260..2907
                     /locus_tag="PLC06_07245"
     CDS             2260..2907
                     /locus_tag="PLC06_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRO90278.1"
                     /translation="MSAVSTTPASHDDAHAGGHEVPYSTQLRSNRLGLWLFMFSELFL
                     FGALFTTRFVLWGNTRPNLSQELGLLATVILLISSFFMYRSETAIANGDQKTFLRSAL
                     VAATMGVIFFIMVVVMEWNIFGLSGSLFGIELFGHLKPSDGVYGGVFYAMTGMHALHV
                     LSGVVFILIIWWNGRRGFFTPERHWGVEATAIYWHYVDVIWVFFYPALYLMGKAV"
     gene            3003..3935
                     /locus_tag="PLC06_07250"
     CDS             3003..3935
                     /locus_tag="PLC06_07250"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRO90279.1"
                     /translation="MDPVARAALSSWDWRPEVIVTILVLGGLFLTGWIRLRAISGPAK
                     NWRSLGAKWRPISYIFGLLVIGFALMSPLDVLVQQLFFMHMIQHLLLIMVAPVFLLLP
                     NPMPFLLWGLPPRARLAAGSVLNSVLNKDSAIGRILRKLTSPFAVWFIMIVFIIGWHD
                     PNMYNAALRYEWVHDLEHVTMFVAGMLFWWTVTGAGPRLHKNLSRPAKVAFVLAAIPP
                     NMALGATLAFTQTPIYSFYSDMPRLWGISVLDDQRYSGIIMWIPGSMMYFMAALALLF
                     LILSGEERKPVPEDKPWLSDDALAAPGISSGRKS"
     gene            3941..4744
                     /locus_tag="PLC06_07255"
     CDS             3941..4744
                     /locus_tag="PLC06_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HRO90280.1"
                     /translation="MGELGRTLFSRRWWWVTLIVLVLMAVFARLGFWQLDRLEQRRAA
                     NAQLIAALESNPIELNDAINEYRLIAPDEISGDLANRDVVVRGTYDFLGQRIVKLQSW
                     NGRAGVHLITPFLIEGSDVAILVNRGWIPDAEYAAGNAYDDETGTQTIEGYVALTETI
                     SRNASDAAAPDDPGAELFRVDIATLSQFMPYPLAPFYLKLDSGDEASADLPIPVPKEV
                     DLSEGPHLDYALQWFVFSAGLGIAYVAYVYRWLRLKKAEETAERKPVQD"
     gene            4900..7158
                     /locus_tag="PLC06_07260"
     CDS             4900..7158
                     /locus_tag="PLC06_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HRO90281.1"
                     /translation="MDPIAGRLREDLPSLFLIWGMLLIASAAVLQADLVDGMHILPIV
                     VTLALVAGWLLARSIFSDRTAHLFALIYGLFFVFFLVGSTLPYEGPWRERVLDLLGRQ
                     MDWLQKAFSGGTSRDGIIFVIQTSALFWILGYLAAWYTFRRVHVWRVIVPIGVVLLSV
                     VYYYNGPRPLLIYLAIFTLLALMLVAITHLTAEQVEWRKASVRFDRAIQFDFLRAGLV
                     AALLALLLAWSLPGLQASSMVGDAMSSPGGPWRAFQDTWTRLFSSLRSYGAGTSDPYQ
                     DSLVLGGPRSVGQTLIMDIQVTKELPYGVYWQAIVYDTYKDGGWKARESDGEELIHYP
                     EDGLLNVPLSLSRTVITQTVTNYLPNSSFLYAAPEAVGSDRQILVDGTLDPNGKTLVT
                     SLRSRYVLRQGDQYRVSSRVSVADATSLQADTVDYPQWIRDRYLQLPDTITARTIALA
                     EELTAGLTNPYDKAIAVRDYLRANIRYNDQINAPPEGVDPVDYVLFDLKEGYCTYYAS
                     AMAIMLRSQGVPVRLVSGYAQGEYDEPVQSYRVRAENSHTWVEVYFPTYGWIHFEPTQ
                     SIPVAERPSSGTVGTGSGEDIEPVPSGDLFPMDDPTDFESGGGFLDNLGDTAAEQGVS
                     NQVAWWQIALGVLILLMAGAAAFFGYQYNRRIESDVDRSYGRLGDWARWLGAPWRTTQ
                     TPYEQADALVTVIPEGRQPVRNLTRQYVLQRFSPHGTDDEPIDPLQEWKQLRPLLIKR
                     RLANFINRSRDK"
     gene            7292..9025
                     /locus_tag="PLC06_07265"
     CDS             7292..9025
                     /locus_tag="PLC06_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRO90282.1"
                     /translation="MKKQSSEMPAADFQYLLAQLQQLQATLSEQQRTLLRVPDGQAKA
                     TAIRGSGVLTRRLDALSETAGQLQKESARQEIEKERLLALQEVGAAINSSLDLDVVLQ
                     QVMDAIIRLTNAERAMLLLVNDGDLEVKIARNISQETLDNAASLEISHSIVRKVAESG
                     EPIVSINAQEDERFALQHSIVSYKLRSIVCAPLKIKGETTGVIYADNRVASGIFSDTD
                     RDLLAVFADQAAVAIDNARLFHEVAGMKHLMDNVFASIAGGVITIDENDRIALFNRAA
                     ERILGMSAEPLHLRTYQDVLSLLGLPVESLIHQAKEDGQTHSAELDLGGVRRRNSPVT
                     VNVTVSPLSTVDNERVGGVAIVIDDVSEKKRVESLRRYLPPALVDRVRDLDAAQKPQR
                     RPITVMFADIRDFSRFGEHVDPEELIDLANGLFSEAVAAISAHHGLIDKFMGDAVMAL
                     FNTPLNPQEDHVEQAVAAALDVQARMVAYRSELPSSKLLHFGIGIHTGDAVVGNVGSA
                     QRKDYSAVGDVVNLCKRLQELAGADQIIISRRVYEHVRDYALVESLAPVKLRGRKARE
                     EVYRLLDAGKL"
     gene            complement(9022..>10116)
                     /locus_tag="PLC06_07270"
     CDS             complement(9022..>10116)
                     /locus_tag="PLC06_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRO90283.1"
                     /translation="WLVSRFRTIIGMLVMAVVLSYLMEPIIGFIERRTKIRRSLVIIV
                     VYIILALALISGFSAVGLATFQQFGNLIATLPSLIENTGDSISAFVTRPEPIDLGPFT
                     FSPAIIPWDRLADQLIGMLEPVLTQSTTFVSRFATSTVRTVFNFVTIFIIAVYLSTDM
                     PNFNGYIRSFAQQPGYSEDAARLMPELSRVWRSYLRGQIILGLVIFFMVWIGLTLLGV
                     QNSLALGLLGGLLEFIPTIGPIVSAVVAILVAFFQPANYLGLAAWQYALVVLALMIII
                     QQLENNLLVPRIVGNALDLHPILVIVSVFMGASLGGVLGAILAAPIAASLKVLGQYTW
                     RKLFDLPPFPQTKEPPPEPEPAPASEAAAN"
BASE COUNT         1987 a         2781 c         2943 g         2405 t
ORIGIN      
        1 ggctttaacc cgaatatcat gccccagaat tatctggacc agttcactac ccgcgaacag
       61 gaaattggca ccgccgaggc attcgatctt gatattgccg cggatatcat cgcgtttatc
      121 aagactttgc agtaagcgct gagagcacgc aggagcagaa agatgtttag gaccctaaga
      181 atatttttta cctcatcatt ggtcaaaggt atcgtcgggc agtatgtcgc ctgggcgctt
      241 ggctatggct ttatcgccat gctgcgttcg ttccgcgggc agccgactcc ctggttattt
      301 gaaccggcga ttgcttttgg cgcgctggtt agcgttctgg gtttcctcct ggccgccggt
      361 gtgctgaccg attggctcaa atggatcgtc ggcaagcgca cacctctggc ccatggcgtg
      421 cccgcgggca agccggaatg gtcgcgttat ttcaacgtgg attacaatca caaggtcatc
      481 ggcatccagt atggcgtcac gtccttgctg gtgttgctgg tcggcggcct gtttgccatc
      541 atcttccgca tcgagctggc tcatccgggg atgcaatggc tgaccaatga ccagttcaac
      601 acgctcttca gcgctcatgg catcgtaatg atcgccagca ttctgttggg ggtcggcgcg
      661 atggtgaact acctcgtgcc tctgatgatc ggtgcatccg acatggcctt cccccgcctc
      721 aacgccttta gcttctgggt gaccattccg tctgtgacct tgattctgac cggcatggct
      781 gtcggtggct gggatacggg atgggtgggc tactcgacat tgagcctccg cgcccctctt
      841 ggtatcgtgc tctttctgtt aggcttttgg gtcaacgggt tctcctcaat tgccagctcg
      901 ctcaatatgc tggtgacggc taccacgatg cgggccaagg gcatgacctt gttccgcatg
      961 ccgattgtcg tgtggggcgc ggctgcggcg tcaattatcc aattgaccgc cacccagact
     1021 gttggcgtcg ccttgaccct gaccgtcgcc gagcgcgttt tgggccttaa tttctttgac
     1081 ccaagcggtg gcgggaatcc gattctctat cagcatcttt tctggttcta ttcccatccc
     1141 gttgtctacg tcttcgtgct gcctggcctg gctgtcatca gcgaattgct gccggtcttc
     1201 gttcgcaagc cgcttttcgg ctatcgttgg gttgcgcttt ccagcctggg catcggactg
     1261 gtggggttcc tggtctgggc ccatcacatg ttcacatcgg gtatggccga cgcgctgcgg
     1321 atgcccttca tgttctccac gatgatggtg gccgttccga cgggggtcaa gttcttcagt
     1381 tgggtcgcta ccatgtggga gggcaaaatc cccaggaacc cgcccactcc gctcctgttt
     1441 gtgttcggcg ctatttcgat cttcctgatt ggtggcgtga ccgggccaat cctgggcacg
     1501 attccaaccg acctgcatct gcatgacagc tactggattg tcgggcattt ccatgcgacc
     1561 atgttcggcg gatacatctt cccgttcttc gccgctctct actactggta ccccaaaatc
     1621 agcggccgca tgtacaagga atctctgggc aagctccatt tctggctgat gctccccgct
     1681 ttctgggtga tgagcatcgg tcagatgagc gtcggcttgc tgggcatgcg ccgccgcgtc
     1741 gccgactatg atcccgcgct gggcatcacc acgacgcagc tattggtcac tttgtcggct
     1801 ttggtcatcg gctggtcggt gctgattatg atctacaact tcatctccag cgcgcgcagc
     1861 cagaccgccg cggatatcaa cccgtggcgc tcgcgctcgc ccgagtggca gattccgtca
     1921 ccggtgccgg agttcaacta cagcgttccg tttgaggtag tgggagagcc atacgattat
     1981 ggcctggctg attctatcta cacacgagac ttggtacccg cgccatcagg cgactagaag
     2041 gggagacgga taagaaggat gaacgataaa aaagcggccg cctatcgcct gggcttgatc
     2101 acattggtga ttttggcggt tctaacgatt gtcgaattta ttatcgctgt gtacatgggt
     2161 gcgctggtat tgctgttcat cattgccctg ttgaaagcgg ccgtgattat ccagaatttc
     2221 atgcatatcg cgcggttatg gcaagaggag agtcactaaa tgagcgctgt atctacgacg
     2281 cctgcgtccc atgacgacgc gcacgccggc gggcacgaag tcccctactc gactcaactc
     2341 cggtcaaatc gcctggggct gtggctgttc atgttttctg agctgttcct ctttggcgcg
     2401 ttgttcacca cgcgattcgt gctctgggga aacacgcggc cgaacctgag ccaggaactg
     2461 ggcttgctgg ccacggtgat tttgctgatc agcagcttct tcatgtatcg cagcgagacc
     2521 gcgatcgcca acggcgacca aaagacgttc ctgcgcagcg ctctcgtagc ggccacgatg
     2581 ggggttatct tcttcatcat ggttgtcgtc atggagtgga atatttttgg gctgagcggt
     2641 tcgttgttcg gtatcgaact cttcggccat ctcaagccat ccgatggcgt ctatggcggc
     2701 gtgttctacg ccatgacggg catgcacgcc ttgcacgttc tgagcggggt ggtgtttatc
     2761 ttgatcatct ggtggaacgg tcgtcgcggg ttcttcaccc ccgagcgtca ttggggcgtc
     2821 gaggccacag cgatctactg gcactatgta gacgtaatct gggtattctt ctacccggcg
     2881 ctgtatctga tggggaaagc cgtctaatcc tcagcttttt attgaggcca cggctgaata
     2941 ttgagtaagc cgtggcctgg tttaattccc ctttccgagc caccctcgct acaatcacaa
     3001 atatggatcc tgtcgctcgg gcggcgctct cctcgtggga ttggcgccct gaagttatcg
     3061 tcaccattct cgttctgggc gggctgttcc tgaccggctg gatcaggctc cgcgcgatca
     3121 gtggccccgc gaagaactgg cgctcgttgg gggccaaatg gcggccgata tcctacatat
     3181 ttggcctcct ggttatcggg ttcgccctga tgtcgccgct ggatgtgctg gtgcagcagc
     3241 tcttcttcat gcacatgatc cagcatctcc tgttgatcat ggtcgctccg gtcttcctgt
     3301 tgctgcccaa tccgatgcct ttcttattgt ggggtctgcc tcctcgcgcc cggttggctg
     3361 ccggctcggt gctcaatagc gtgctcaaca aggactccgc tatagggcgg atccttcgta
     3421 aattgaccag cccttttgcc gtgtggttca tcatgatcgt gttcattatc ggctggcacg
     3481 acccgaatat gtataacgcg gccttgcgct acgaatgggt gcatgatctg gaacacgtca
     3541 cgatgttcgt tgccgggatg cttttctggt ggacagtgac cggggcaggg ccgcggctgc
     3601 acaagaatct gagccgcccc gccaaggtcg ctttcgtgct ggcggcaatc ccgcccaata
     3661 tggctctggg agccaccctg gcgttcaccc agacgccgat ctactccttc tacagcgata
     3721 tgccgcgctt gtggggtatc tccgtcctgg acgaccaacg atatagcggg attatcatgt
     3781 ggatccccgg cagtatgatg tacttcatgg cggcattggc gctgctcttc ctcatccttt
     3841 ccggcgaaga gcgcaagccg gttccggaag acaagccatg gctgtctgat gatgctctgg
     3901 cagctcccgg catatcgagc ggcaggaaat catgaacacc atgggtgaac ttggacgaac
     3961 gctattcagt cggcgttggt ggtgggtgac gctcatcgtg ctggtgttaa tggccgtgtt
     4021 cgcgcgtttg ggcttctggc aattggacag gcttgagcaa aggcgagcgg ccaacgccca
     4081 gctaatcgcg gcgcttgagt caaaccccat tgaactaaac gatgcgatta atgagtacag
     4141 actcatcgcg cctgatgaaa tctccggcga tctggcgaat cgtgacgttg tggtcagggg
     4201 gacttatgat tttttggggc aacggattgt caagctccaa tcctggaacg gccgcgccgg
     4261 cgtccatctg attacgccgt ttctcatcga aggctctgat gtcgccattc tggtgaacag
     4321 gggatggata cccgatgccg aatacgccgc ggggaatgcc tatgatgatg aaaccggcac
     4381 acaaacgatc gagggatacg tcgcgttgac ggagactatc agtcggaacg cctctgatgc
     4441 ggccgcgccc gatgaccccg gtgctgagct ctttcgggtc gatatcgcca ccctgtccca
     4501 attcatgccg tatcccctgg ccccttttta tttgaagctg gattccgggg atgaggcgtc
     4561 ggccgatctg cccatccctg tcccgaaaga agttgacctt tccgaagggc cgcacctcga
     4621 ttatgcattg cagtggtttg tcttttctgc gggtttgggc attgcctacg tggcctacgt
     4681 ttatcgttgg ttgcgattga agaaggcaga agagactgcc gagcgcaagc cggttcagga
     4741 ttaagcctct ctaacaaaat tgtcaccata ttgacacccg gcgaccactc gctagaatag
     4801 cgattgctcg atcgggtacc gatttagaaa tcaataacat ctttatttcc atcgatttct
     4861 cgacgtcaag acggtatcgc aaccacagga gaacgccgca tggaccccat cgccgggcga
     4921 ctacgagagg atttgccatc cctttttctg atatggggga tgctgctgat cgccagcgct
     4981 gcggtgctgc aagctgatct ggtggacggc atgcacatcc tgcccatcgt cgtcaccttg
     5041 gcgctggtgg cgggctggtt actcgccagg agcattttct ccgatcgcac ggctcatctc
     5101 tttgccctga tctacgggct attcttcgta ttctttcttg taggatcgac gctgccgtat
     5161 gaaggcccct ggcgcgaaag ggtattggac ctcctggggc gtcagatgga ttggttgcag
     5221 aaagctttca gtggcggcac cagccgggac ggcatcatct ttgtcatcca gacctcagcc
     5281 ctgttttgga ttcttggcta cctggctgcc tggtatacat ttcggcgggt gcatgtctgg
     5341 cgagtcatcg ttcccatagg tgtcgtcctg ttgagcgtgg tctattacta taatgggcca
     5401 cggccgctcc tgatttatct ggctatcttt actcttctgg cgttgatgct tgtcgccatc
     5461 acccatctca cggccgagca ggtggaatgg cgaaaggcgt cggtgcggtt cgatcgcgcc
     5521 atccagtttg atttcctgcg cgcgggtctc gtggccgctt tgctggcgtt gcttctcgcc
     5581 tggagcctgc cgggcctgca ggccagctcg atggtcggcg acgcgatgtc cagccctggt
     5641 ggcccctggc gcgcttttca ggatacctgg acccggctgt tctcatcctt gcgttcctat
     5701 ggggccggaa ccagtgatcc gtatcaggat agtcttgtgc ttggcgggcc gcgatcggtc
     5761 ggccaaacgc tgatcatgga tattcaggtc accaaggaac tgccctatgg cgtttactgg
     5821 caggcgatcg tctatgacac ctataaggat ggcggctgga aagcgcgaga gagcgatggc
     5881 gaggagttga ttcattatcc agaagacggc ctgctcaacg tgccgttgtc gctgtcccgc
     5941 acggtgatca cccagacggt taccaactac ctgccgaact ccagctttct ctacgccgct
     6001 cccgaggcgg ttggcagtga ccgccagata ctcgtcgatg ggacattgga cccgaacggc
     6061 aagacgctgg tgacttcgtt gcgctcacgc tatgtcctca gacagggcga tcagtatcgc
     6121 gttagctccc gtgtttcggt tgccgacgcc accagcttgc aggcggacac cgtggattac
     6181 ccccaatgga tacgagatcg ctatctgcag cttcctgaca cgatcaccgc aagaacgatc
     6241 gctttggctg aagagctgac ggccggcctg accaacccct atgacaaagc tatcgctgta
     6301 cgcgattatc tgcgcgccaa cattcgttat aatgaccaga tcaatgcccc gcctgagggt
     6361 gtggacccgg tcgattacgt gttgtttgat ctgaaagagg ggtattgcac gtattatgct
     6421 tccgccatgg ccattatgct tcggtcgcaa ggcgtgccgg tgcgattggt gagtggctat
     6481 gcccagggtg agtatgacga accggtgcaa tcctatcgcg tgcgcgccga gaactcccat
     6541 acctgggttg aggtgtattt tcctacctat ggctggattc attttgagcc gacccaatcg
     6601 attccggtag ccgaacggcc gtcttccggt actgttggaa caggctctgg ggaagatatc
     6661 gaacctgtac cgtccggtga cctgttcccg atggacgatc cgacagattt cgagtcgggc
     6721 ggcggattcc tcgacaacct tggcgacacg gcagctgagc agggtgtgtc caaccaggtt
     6781 gcctggtggc aaattgcgtt aggcgtgctc atactgctca tggcgggggc cgcggctttc
     6841 tttggctacc aatacaatcg ccgcattgaa tccgacgtgg atcgcagtta tgggcgattg
     6901 ggcgattggg cgcgttggct gggtgccccc tggcgcacca cgcagacccc ttatgaacag
     6961 gcggatgcgc tggtgactgt tattccggaa ggccggcaac ccgttcgtaa tctgacccgg
     7021 cagtacgtcc tccagcgatt tagtcctcat gggactgatg acgagcctat cgaccccttg
     7081 caggaatgga agcaattacg gccgctgttg attaagcgca ggttagccaa cttcatcaat
     7141 cgttcgcgag ataaatagtt gcggtaactc cgccgcgaaa tttctattac tgcacgacct
     7201 gatataatgg cgcgggaata gctttatccc cttcgtgggg ttgctccagc tgccggtggg
     7261 gtgggcctat ggggattgaa cggactacgt tgtgaaaaag caatcatcgg aaatgcccgc
     7321 ggccgacttc caatacctcc tggctcagct ccagcaactt caagccaccc tcagcgagca
     7381 acagaggaca ttattgcgag tgcccgacgg gcaggccaaa gcgacggcga ttcgcggcag
     7441 tggcgtgttg acccgccgac tcgacgcact gagcgagact gccggacagt tgcagaaaga
     7501 gtcggcgcgg caggagatcg agaaagagcg gttgctcgcc ttgcaggaag ttggcgcagc
     7561 tatcaattca tcgcttgacc ttgatgtcgt gttacagcag gtgatggacg ccattatccg
     7621 gctgaccaac gctgagcgag cgatgttgtt gttggtcaac gacggcgatt tggaagtcaa
     7681 gatcgctcgt aatatctcgc aggagacgct ggataacgcc gcttccctcg agatcagcca
     7741 cagtattgtg cgcaaagtgg ccgagtcggg agagccgatt gtctctatca atgctcagga
     7801 agacgagcga tttgccttgc agcacagcat cgtcagttac aaattacgtt ccatcgtctg
     7861 cgcgccgctc aagatcaagg gcgaaacgac gggcgttatc tatgcggata atcgcgtggc
     7921 cagcggcatt tttagcgata cggatcggga tttgttggct gtgtttgccg atcaggcggc
     7981 cgttgccatc gataacgcgc gactctttca tgaagtggcc ggcatgaaac acctcatgga
     8041 taacgtgttc gcttcgattg ccggcggcgt catcacgatt gatgaaaatg accggattgc
     8101 tctgtttaat cgcgcggccg aacgtatcct gggaatgagc gcggaacctc ttcatctacg
     8161 aacctatcag gatgtgctgt cattgcttgg tctgccagtc gaatctctga tccatcaggc
     8221 caaggaagac gggcaaaccc atagcgctga gttggacctg gggggcgttc gtcgaagaaa
     8281 ttcccctgtt accgtcaatg taacggtatc gcccctaagc actgtcgata atgagcgtgt
     8341 gggtggggtg gctatcgtga ttgatgatgt ttccgagaaa aagcgcgtag agagtttgcg
     8401 ccgatatttg cccccggcgc ttgttgatcg agttcgcgac ctggacgcgg cacaaaagcc
     8461 tcagcgacgg ccgattaccg tcatgttcgc ggatattcgc gacttcagcc ggttcggcga
     8521 gcatgtggat cccgaagagt tgatcgacct ggctaacggc ctattctccg aggcggtggc
     8581 agccatcagc gcccatcatg ggctgatcga caagttcatg ggcgacgcgg tgatggcctt
     8641 gttcaacacg ccattgaacc cgcaagagga tcatgtcgag caggcggtcg ccgccgcgct
     8701 ggatgttcag gcacggatgg tggcctaccg cagcgagttg ccgtcgagca agctccttca
     8761 tttcggcatc ggcatccaca ccggtgacgc agtggtgggg aatgtgggca gcgcccagcg
     8821 caaggattac tcggccgtgg gcgatgtcgt taatctatgc aagcggctgc aggaactggc
     8881 cggcgcggat cagattatca ttagccgccg tgtttatgag cacgtacgcg attatgctct
     8941 ggtggaatca ctggcgcccg tcaagctgag aggcaggaaa gcgcgagaag aagtttatcg
     9001 tttgctggac gcaggtaaac tctagttagc cgcggcctcg gatgcgggag ccggttcagg
     9061 ctcaggggga ggctctttag tctgagggaa tggcggaagg tcgaacagct tgcgccaggt
     9121 gtattgtccc aatactttca atgaggcggc gattggcgca gccagaatcg cgcccagaac
     9181 accgcccagt gacgccccca tgaagacgct gacgatgacc aggataggat gcaggtcgag
     9241 cgcgttgccg acgatgcgcg gcacaagcaa gttgttttcc aactgctgga tgatgatcat
     9301 cagagccaga acgaccaaag catattgcca cgcagccagc cccagataat tcgccggttg
     9361 gaagaacgcc accagaatgg ccaccacggc gctaacgatt gggccgattg tagggataaa
     9421 ctccagcagg ccgcctaaca ggcccagcgc caatgagttc tgaaccccca gcagcgttag
     9481 accaatccat accataaaaa agatcacaag tcccaggatg atctgcccac gcagatagga
     9541 gcgccatacc cgactgagtt ccggcatcag gcgcgcggca tcttcgctgt agcccggctg
     9601 ttgggcgaag cttctgatgt agccgttaaa gttgggcata tcggtggaaa gatacaccgc
     9661 gatgataaat atggtcacaa aattgaacac cgtgcgcaca gttgatgtgg cgaaacggct
     9721 gacgaacgtc gtgctttggg tcaacaccgg ttccagcata ccaatcaact gatcggcaag
     9781 gcgatcccag gggataatcg cggggctaaa cgtgaaaggg cctaaatcaa taggctccgg
     9841 acgtgtaacg aaggcgctaa tggaatcgcc ggtgttttcg ataagactgg gaagggtggc
     9901 aatgaggttg ccaaattgct ggaatgtggc aagccctacc gcggaaaagc cgcttatgag
     9961 tgccagggcc aggatgatat acactactat gatgaccaaa ctgcgacgga tcttcgttcg
    10021 tctctcgatg aaaccgatga ttggctccat cagatagcta agcacgactg ccatcaccaa
    10081 catgccgata atcgtgcgaa aacgactgac cagcca
//
LOCUS       DAOMQG010000098        10048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2896_length_10048_cov_9.758131, whole genome shotgun sequence.
ACCESSION   DAOMQG010000098 DAOMQG010000000
VERSION     DAOMQG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_10048_cov_9.758131"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..2336)
                     /locus_tag="PLC06_07275"
     CDS             complement(225..2336)
                     /locus_tag="PLC06_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90284.1"
                     /translation="MSGKKKEHGRLNSPLHPFTPSPLHSLLSILLIALSAIWLYRRAL
                     GFAYFNDDPTGHFAWMEGQSLWQFFAGSADYGYYRPIVFVVLRLSELLFGNETFPHNP
                     VADHALLLLLHAANAVMAWLLARRLSGRDIFAWAAALVFAFVPFNYEAVAYVASLTHP
                     LVVFWMLAALLLYGGMLTADGRRDSCRATAAREPERKGPGHWSLTTGHWPLVIILLLM
                     TLGLLTHENGLFIFPALVGMDWMLRPGSPLRKRVGRLWMFAVPPALFAALWVLIPKSS
                     EQGLNSLADIGRNLVPFLQTLVFPLLPVIRLDAGDVWPLVLLVLVVVAGLGWIAWRAG
                     AGRLWVFALGWFSFSSLPALLFLGPAYVYGSPRLSYLPSIGVALLWGLPVLWPASGLL
                     SADRERPPDRALPSSFVLRISHFVTSIAFIIAILLPPLPFIRCQLDFYDETSRFARGM
                     AAAGSAAPVGREVVFINAPFFFSSTAARPEGCPSPYPWTPIGGVLIPPYAQARDFVRF
                     NGGPDRSVAGVSYPGYNPGWRTFGPEIDGARLREHAAADATYVFDLMRGTFADMAAAW
                     QPGAGEVADPAATFGDALVLVGAELWPEPERLVVGLDWRVTGKSAAPLAAFVHVYDGS
                     GALVAQSDGPPGAGLAPQALWATGDGLADERVIDVTTLPPGSYSVAVGVYNTTDGARL
                     TAMAAGMPLAENVYTIGMLER"
     gene            2597..3907
                     /locus_tag="PLC06_07280"
     CDS             2597..3907
                     /locus_tag="PLC06_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90285.1"
                     /translation="MRPSSIRPTLMRGVAIFLLILIGLAIAPQTLFAQAVIRGGNLYP
                     GEVIDNDVIMTGDAVQLSGTVKGNAFISGRDVVIDGTVEGSLFVIGQRVTINGTVGGG
                     AYVLSISSRLGAAGSVGQNLYYLGVSFVSERGSRVGRDLVGLSLGAVLQGSVGRETRL
                     IAGLLQFVNLFFDFALGPTPAPLVTAIAGRAPGLGQIILPGEVVIDVFGQTVNTAQPP
                     VPPEPPTQSELVLDWLMARLREFLPMLIVGLIGYWFLRDRLERSVVPIKTHPLITLGY
                     GLVGLVLVVAVITALLLVFFLIFMTGMWIGRTTFWNVAWLFWSIAFPLTALVFSLLMA
                     FFNYGTKAITSYALTGYLVDRFIPRAGRHRWLLMTLGLIVYVCLRSIPTLGWVIAVLV
                     TSWGIGGAWLAWRVRRPMPLFYTAEPGLAETAPDDAEKTDDPTG"
     gene            3965..4522
                     /locus_tag="PLC06_07285"
     CDS             3965..4522
                     /locus_tag="PLC06_07285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRO90286.1"
                     /translation="MVKSWQKTGSEYLGDFRIFRIRADSSRSPRTGQVHRFFVLESPD
                     WVNVIPLTPEGNVVMIRQFRHGTEDVTLEVPGGMVDREDGNPSVSAARELREETGYEA
                     ATIVHLGSVAPNPAFLNNHCHSYLARDVRPVASPQLDGAEDISFEEIPLAAIPGLIRD
                     GTITHSLTIVAFYYLELMGQMTADG"
     gene            complement(4629..5744)
                     /gene="asd"
                     /locus_tag="PLC06_07290"
     CDS             complement(4629..5744)
                     /gene="asd"
                     /locus_tag="PLC06_07290"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRO90287.1"
                     /translation="MNKIPVGILGATGTVGQRFVQLLADHPWFEVVVVTGSDRTVGRP
                     YGEGVNWLMPGDVPPATAALVVQPTGLDLNIPGTPPVVFSALPTAEAKVWEPQFAAAG
                     YAVVTNTSVFRMDPLVPLLIPEVNPDHTSLIPTQQAEYNWPGFIVASPNCSTTSAILP
                     LKVLQEAFGVEAAIITTLQAISGAGYPGVSSMDIYDNVVPYIGGEDDKLQNEPTKLLG
                     VVEDGRMIPAGLSLSAQANRVPVVDGHLASVSVKLGRRVSADAAIAALRAWQPPAITA
                     QLPSSPKELLIYREEPDRPQPRRDRDAGDGLAWTVGKVRECPVLDVRYVSLTHNTLRG
                     AASGALLNAELLVVQGFIGRDLAISSNGHQPAAAHAF"
     gene            complement(6151..6232)
                     /locus_tag="PLC06_07295"
     tRNA            complement(6151..6232)
                     /locus_tag="PLC06_07295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6196..6198),aa:Leu,seq:gag)
     gene            complement(6325..7035)
                     /gene="nth"
                     /locus_tag="PLC06_07300"
     CDS             complement(6325..7035)
                     /gene="nth"
                     /locus_tag="PLC06_07300"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRO90288.1"
                     /translation="MEDSERTKDFEALREKYDTVYRILIEVYGRPERGTPGPALNELV
                     GTILSQATTDANTDRAYAELVARFPDWKSVMDAPLEEVIAAIRSAGLANTKGPRIQAA
                     LRAIYQERGELSLDFLADMPVPEAMAWLTRLKGVGPKTASIILLFSFGRAVFPVDTHV
                     HRVTGRLGLIPPRMSANQAHEFLAALGAPETYYPMHINLIRHGREICHARSPQCQICP
                     LQDYCDYYHNVFLGAADG"
     gene            complement(7040..>8305)
                     /locus_tag="PLC06_07305"
     CDS             complement(7040..>8305)
                     /locus_tag="PLC06_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90289.1"
                     /translation="FLREGGRLGFVVPNGWLDVSYGEGLKRFLLDHFRIVAVIESAVE
                     RWFSPARVNTCLVILERADDPESRKANRVRFVRLRQPLHELLGQDVDSRRMAAVEQLV
                     TRLMPASDRVTPGASVRVREQGALTAFGRWGALLRAPEVYLHRHARPVAPLAEWVTVQ
                     RGYTTGANDFFYPDRKQVERWGIEPEFRRPLLKSLRGVRRLRLSAEDCRHEVLAIPAD
                     ADLTGTAVAEYIGWAESQGVAQRPTCVGRRPWYALPEQPDAALVLAKGVWQRHFAPAV
                     GGGIVVDQQIYRVELADGIMLNEAAALLNSAWFALACELGGRVNLGEGVLWLAAYELA
                     KILLPDPRSLDAAARRELSSCFEALAALPVVDTAEALERPQQRVLDDMVFDLVGLPPA
                     ERDAARTALLDCLSGRRLRARTPIMTKEK"
     assembly_gap    8306..8405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8406..8694)
                     /locus_tag="PLC06_07310"
     CDS             complement(<8406..8694)
                     /locus_tag="PLC06_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRO90290.1"
                     /translation="MTSKQRAWGQFVTPIDVADLLLGFCLRRSDDRLLDPGCGDGALL
                     RRAALWRSWLAADRPEEGGVLYGIELDPEAALAATIIPGAVIERANFFTLDP"
     gene            8757..9227
                     /locus_tag="PLC06_07315"
     CDS             8757..9227
                     /locus_tag="PLC06_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90291.1"
                     /translation="MTAISQKTFFDTLPDALRPSLPVALQGFQVRRPWGLLQIHFGEP
                     ALHYEVSYVGQRLGRLITGWELGFHCEARDHHLNRLLLDGFSHHLFEIKATLGEHVEA
                     EMWDRGWTKIYEVYPDAPLTQAYRADVATRLAEFITCIHPIFVGLRRSAAEIYR"
     gene            complement(9279..>10048)
                     /locus_tag="PLC06_07320"
     CDS             complement(9279..>10048)
                     /locus_tag="PLC06_07320"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90292.1"
                     /translation="RITVPEGATHNLWGDNKDAPRVMQAADNVDFQYVVKFDSTVTQT
                     AQMQGIIVEGDAQNFARFEIQYNNGLKAYAATIEGTKSKARIAVDIVNPADAVYLRVT
                     RTGEIWKMEYSGNGATWIEAGSFNKYTLNVTKAGIFAGNVQGKNSPAPAHTAVVDYFH
                     NATLGPMSAERPLLDITIVGSGSVTANPPIGQLTCGANVTLTANPGLGATFSNWSGAV
                     TGTQPTISFLINGPKQVTATFSDGGMQYSLLPIIIKK"
BASE COUNT         2019 a         3232 c         2931 g         1766 t
ORIGIN      
        1 ccttcctggc atcgatcatc cccgcccacg ccgccggccg catcagcatc cgcgaaagcc
       61 tggcctacgg gtagaaacaa aaaacgccta taaacgcgta cagccccggc caggaggggg
      121 aaccggggct gtacgcattc ttggcgactt catggataag tctatccgcg gttcctaaat
      181 ttagggtgag ccgaaggtga gcggttggtg agggagcggt ggggtcatct ctccaacata
      241 ccgatggtgt atacgttctc ggctaaaggc atcccggcgg ccatggccgt cagccgcgcg
      301 ccatcggtcg tattatagac acccaccgca acgctataac tccccggcgg cagcgtcgtc
      361 acatcgataa cccgttcgtc ggccagccca tccccggtcg cccacaacgc ctgcggggcc
      421 agcccggccc ccggcggccc atcgctctgg gcaaccagcg ccccactgcc atcatagaca
      481 tggacgaagg cggccagcgg cgcggcgctc ttcccggtca cacgccaatc cagccccacg
      541 acgaggcgct ccggctccgg ccatagctcc gccccgacca acaccagcgc gtcgccaaac
      601 gtcgccgccg gatcggccac ctcgcccgca cccggctgcc aggcggccgc catgtccgca
      661 aatgtacctc tcatcagatc gaatacataa gtcgcgtcgg ccgccgcatg ttctcgcaat
      721 ctcgcgccat caatctccgg cccgaacgtg cgccagccgg ggttatagcc gggataggag
      781 acgccggcca cgctccggtc ggggccgccg ttgaaacgca caaagtcgcg cgcctgggca
      841 tagggcggga tgagcacgcc gccgattggc gtccatggat agggagacgg gcagccttca
      901 ggccgggcgg ccgtggagct gaaaaagaac ggcgcgttga tgaacaccac ctcgcgcccg
      961 accggagcgg ccgacccggc cgccgccatc ccgcgcgcaa accggctggt ttcgtcgtag
     1021 aaatcgagct ggcagcggat gaagggcagt ggaggcaaga ggatggcaat gatgaaggcg
     1081 atggaagtga cgaagtgcga aatacgaagt acgaaggacg aaggaagagc gcgatctggc
     1141 ggccgttcgc ggtcggcgga caggagtccg ctcgccggcc acaacaccgg caatccccac
     1201 agcaacgcca ccccaatcga cggcagatag ctgagccgcg ggctgccgta gacataggcc
     1261 gggccgagga agagcagcgc gggcagcgat gagaaggaaa accagcccag agcgaagacc
     1321 cacagccgtc ccgcacccgc tcgccatgct atccagccga gacccgccac gacaacgagc
     1381 accaacagca ccaatggcca cacatcgccg gcgtcgagcc ggatcacggg cagcagcgga
     1441 aacacgagcg tttgcaggaa gggaacgagg ttgcggccga tgtcggccag actgttcaag
     1501 ccctgctcgc tgctcttggg gatcaacacc cacagcgcgg caaacagcgc cggcggcacg
     1561 gcgaacatcc acaggcggcc cacccgtttg cgcagcggcg aaccgggccg cagcatccaa
     1621 tccatcccca ccagcgcggg aaagatgaac aggccgttct cgtgggtcag caggccgagg
     1681 gtcatgagca ggaggatgat gaccagtggc cagtggccag tggtcagtga ccagtggcca
     1741 ggacccttgc gttccggctc tcttgcagcg gtcgcgcgac aggagtcgcg tcggccgtcg
     1801 gcggtcaaca tccccccata cagcaacaat gccgccagca tccagaacac caccagcggg
     1861 tgcgtgagcg aggcgacata ggccacggcc tcataattga acgggacgaa ggcgaaaacc
     1921 agcgcggcgg cccaggcgaa gatgtcgcgg ccgctgaggc gtcgggccag cagccaggcc
     1981 atcaccgcgt tggccgcgtg gagcaggagc agcaacgcgt gatcggccac cgggttgtgg
     2041 gggaaagttt cgttgccgaa tagcagctcg ctcagacgca acacgacgaa gacgatcggc
     2101 cgatagtaac cataatcggc cgacccggcg aagaactgcc acagcgattg cccttccatc
     2161 caggcgaaat ggccggttgg gtcatcgttg aaataggcaa accccagcgc gcggcgatag
     2221 agccagatag cgctcagggc gatgaggagt atagagagca gggagtgaag gggggaaggg
     2281 gtgaaggggt gaagggggga attgagtctc ccatgctcct ttttcttccc actcatgggc
     2341 aattcactca tggcgagggg atgcaaaacg catcatccgg ctatcggttt gggcggcaac
     2401 ggattcccgc atcataagtc tctcataccg ctttccagta aattacccgg cccgggttca
     2461 attacattag cacggatttg ggcgactcgc cacaccatca ggcccccata tcaacactgc
     2521 gattgttcca tcgccgacaa tccaatagaa tacccggagt tatcgacgat tcagcgagac
     2581 tcacaggagc cattgtatgc gtccttcttc aatacggccg accctcatgc gcggggtagc
     2641 catcttcctg ctcatcctta tcgggctggc catcgccccc cagaccttgt tcgctcaggc
     2701 ggtcatccgg ggcggcaatc tctatccggg cgaggtgatc gacaacgacg tcatcatgac
     2761 cggcgacgcc gtgcagctat caggcacggt caagggcaac gccttcatca gcggtcgcga
     2821 cgtcgtcatc gacggcacgg tcgaaggcag ccttttcgtc atcggccagc gcgtcaccat
     2881 caacggtaca gtcggcggcg gcgcctacgt cctgtccata tcatcccgtt tgggcgctgc
     2941 cggcagcgtg ggccaaaacc tctactatct cggcgtcagc ttcgtctccg aacgtggctc
     3001 gcgtgtcggc cgcgacctgg tcggcctcag cctcggcgcg gtgctgcaag gctcggtcgg
     3061 ccgcgagacc cgcctgatcg ccggtcttct gcaattcgtc aacctgttct tcgactttgc
     3121 cctcggcccc acgcccgccc ccctggtcac ggcaatcgcc gggcgcgccc ccggcctggg
     3181 ccagatcatc ctgcccggcg aggtcgtcat cgacgtcttt ggccagaccg tcaatacggc
     3241 ccagccgccc gtcccaccgg aaccgcctac ccaaagcgaa ctcgttctcg actggctcat
     3301 ggcccgcttg cgcgaatttc tgcccatgct catcgtcggc ctcatcggct actggttcct
     3361 gcgcgaccgg ctggaacgat ccgtcgtgcc catcaaaacc catccgctga tcacactggg
     3421 ctatggactg gtcggcctcg tccttgtggt ggccgtcatc accgcgctcc tgctcgtctt
     3481 cttcctcatc ttcatgaccg gcatgtggat cggccgcacg accttctgga acgtcgcctg
     3541 gctattctgg tccatcgcct tcccgctgac ggcgctcgtc ttcagcctgc tgatggcgtt
     3601 cttcaactat ggcaccaagg ccatcaccag ctacgcgctg accggctacc tcgttgatcg
     3661 cttcatcccc cgcgccggac gacatcgctg gctgctcatg accctgggcc tgatcgtcta
     3721 cgtctgcctg cgctccatcc ccaccctggg ctgggtgatc gccgtgctgg tgacttcctg
     3781 gggcattggc ggggcgtggc tggcatggcg cgtccggcgg cccatgccgc tcttctacac
     3841 cgccgaaccc ggcctcgccg aaacagcgcc cgacgacgcg gaaaagacag acgaccccac
     3901 cggctgatcc cgcgacggcc gcaccctttc ttgcgtattc gtcacgcctg cttaaacttg
     3961 cgtcatggtc aaatcatggc aaaaaacagg cagcgagtat ctgggcgact ttcgcatttt
     4021 ccgcatccgc gccgacagca gccgctcgcc gcgcacgggc caggttcacc gcttcttcgt
     4081 gctggaatcg cccgactggg tcaatgtcat ccccctgacc ccggaaggca acgtcgtgat
     4141 gatccgccag ttccgccacg gcaccgagga cgtgacgctg gaagtgcccg gcggcatggt
     4201 cgaccgcgag gacggcaacc cgtcggtttc ggccgcccgc gaactgcgcg aggagaccgg
     4261 ctacgaggcg gcgacgatcg tccatctggg cagcgtcgct cccaatccgg cattcctcaa
     4321 caatcactgc cactcctatc tggcgcggga cgttcggccg gtggcttcgc cccagcttga
     4381 cggcgcggaa gacatcagct tcgaggaaat acccctggcc gccatccccg gtctcattcg
     4441 cgacggcaca atcacccatt cgctgaccat cgtcgccttc tattatctgg agcttatggg
     4501 acagatgacc gctgacggat gaccgccgac cgctaagaga ggttcgtgca ggggtgcgag
     4561 agcgacaagt aatgaactgt gaatgaaggg atcaaaaaga ggcaggtttg aaacctgccc
     4621 ctactttatt aaaaggcgtg cgcggcggcc ggttgatggc cattggatga tattgcaaga
     4681 tcgcggccga tgaacccctg cacgaccagt agttcggcgt tgagcagcgc gccgctggcc
     4741 gctccgcgta acgtgttgtg cgtcagcgac acgtagcgca catcgagcac cgggcactcc
     4801 cgcaccttgc ccaccgtcca ggccagccca tcgccggcgt cgcgatcgcg acgcggttgc
     4861 ggtcgatcag gctcctcgcg gtaaatgagc aactccttgg gtgatgaagg caattgagca
     4921 gtgatggccg gcggttgcca ggcgcgcagg gcagcgatgg ccgcgtcggc cgagacacga
     4981 cggccgagct tcaccgacac gctggccaaa tggccgtcca ccaccggcac gcgattggcc
     5041 tgggcgctga gagagaggcc ggcgggaatc atgcggccgt cttccaccac acccaacagc
     5101 ttggtcggct cattctgcaa tttgtcgtcc tccccgccaa tatagggcac gacgttgtcg
     5161 tagatatcca tcgacgagac gccgggatac cccgcaccgg agatggcttg cagcgtggtg
     5221 atgattgccg cctccacgcc gaacgcctcc tgcaagactt tcaacggcaa gatggcgctg
     5281 gtcgtcgagc aattagggct ggcgacgatg aaaccgggcc agttgtactc cgcttgctgg
     5341 gtgggaatga gcgaggtgtg atcggggttg acttcgggga tcaacagcgg caccagcgga
     5401 tccatgcgga agacggaggt gttggtgaca accgcgtaac cggcggccgc gaactgaggc
     5461 tcccacactt tagcctcggc cgtgggcaag gcggaaaaga ccaccggcgg cgtgccggga
     5521 atattcaggt ccaggccggt cggctggacg acaagcgcgg ccgtcgccgg cggcacgtcg
     5581 cccggcatca gccagttgac cccttcgcca tacggacgac ccacggtgcg gtcggacccg
     5641 gtgacgacca ccacctcgaa ccacggatga tcggccagca attgcacaaa ccgctgtccc
     5701 acggttcctg tcgcacccag gattccaaca ggtattttgt tcatttccta ttctcccaat
     5761 cgccggttaa ttaaccggcg caatttgaac gtgtaatgaa aaacgccatc gtccgcatgg
     5821 ggcgatggcg tcgcgattcc acccaatttc cgcccaacct gtggccgggc gctcgtgcgg
     5881 ccgctaacgg agccaaccgg attcccttac tgacgcgtgg cgttggggaa atcactcccg
     5941 ggtggttttg gccgcggccc caattggcga tgctttcagc ctgtggccat cgcctctctg
     6001 atacgggtgg cgcagttact cgtcccggtc aacgctttaa gtttcgatta tgggatgaat
     6061 tataaggagt ggccgggcgc tgtcaagagg gcaagcgtgc cacgaaaacg aaaagcgccg
     6121 gaaactctca tttccggcgc ttttcctctg tgccctggag aggactcgaa cctccacgtc
     6181 ctaaggacat aggccctcaa cctaccgcgt atgccaattc cgccaccagg gcaacttgtc
     6241 gatattatat cctgatcccg cttgggctgt caatttcgct aatggttcca agcagcacag
     6301 gcaagatcgt ttgacaccgg gcggtcaacc gtcggccgcg ccaagaaaca cattgtggta
     6361 atagtcgcaa tagtcttgca aaggacatat ttggcactgc ggcgaacgag catggcagat
     6421 ttcgcggccg tgacgaatca gattgatatg catcggataa taggtctccg gggcacccaa
     6481 cgcggccaaa aactcatggg cttgattggc gctcattcgc ggcgggatca gccccagacg
     6541 gccggtcacc cgatggacat gggtgtccac cggaaagacg gcgcggccga aagagaacaa
     6601 caagataatc gaggctgtct ttggccccac acccttgagg cgagtcagcc aggccatcgc
     6661 ctcaggcacg ggcatatctg ccaggaaatc gagggacaat tcgccgcgct cctgatagat
     6721 ggctcgcaaa gcggcctgga tgcgcggccc tttcgtgttc gccagccccg ccgaacgaat
     6781 ggcggcgata acctcctcca gcggcgcgtc catgacgctc ttccagtcgg ggaagcgcgc
     6841 aaccagttcg gcataggcgc gatcggtatt ggcgtcggtc gtcgcctgcg agaggatcgt
     6901 gcccacaagc tcgttgagcg ccggcccggg agttcctcgc tcggggcggc cgtatacctc
     6961 aatgagaata cgatagacgg tatcgtattt ctcgcgcagc gcttcaaaat ctttcgttcg
     7021 ctcagagtct tccactcgct catttctcct ttgtcatgat cggggtgcga gcgcgcaaac
     7081 ggcgaccgga caggcaatcc agcaacgccg tccgtgcagc gtcacgttcc gcgggcggca
     7141 accccaccag atcaaaaacc atatcatcca gcacgcgctg ctggggccgt tccagcgcct
     7201 cggccgtgtc gaccaccggc agcgcggcca atgcctcaaa gcaggatgac aattcgcgac
     7261 gtgcggcggc gtccagcgag cgtgggtcgg gcaacaaaat cttcgccagc tcgtaagccg
     7321 ccagccacag cacgccctct cccagattca cccggccccc cagctcacac gccagggcaa
     7381 accaggcgct attgagcaag gctgcagcct cattgagcat tattccatcg gcaagctcga
     7441 cgcgatagat ctgctgatcc acgacaatcc cgcctccaac agccggcgca aaatggcgct
     7501 gccatactcc cttggccaac acaagcgccg catcaggctg ctccggcagg gcataccacg
     7561 gccgccgccc gacgcaggta gggcgctggg cgacgccctg cgattccgcc caaccaatat
     7621 attcggcgac agctgtcccc gtcagatcgg catccgcagg aatagccaat acctcatgac
     7681 gacaatcctc ggccgacagc ctcaggcgcc gcaccccgcg caacgatttg agcaacggcc
     7741 gacgaaattc cggctcgata ccccaccgtt ccacttgctt gcggtccgga tagaaaaagt
     7801 cgtttgcgcc ggtggtgtag ccgcgctgca ccgtgaccca ttcggcaagc ggagctaccg
     7861 gtcgcgcgtg gcgatggaga taaacctccg gcgcgcgcaa cagagccccc caacgcccga
     7921 atgctgtcag cgccccttgc tcgcgcacgc gcaccgacgc gccgggcgtg acccgatcgg
     7981 aagcaggcat gagccgggtc accagttgct cgacggccgc catccgccgg ctatccacat
     8041 cctgacccaa caactcatgg agcggttggc gcaagcggac aaagcggaca cggttggcct
     8101 tgcggctttc cggatcgtcg gcacgctcca gaataacgag gcaggtattg acgcgagcgg
     8161 gcgaaaacca gcgctccacg gccgactcga tgacagcaac gatgcgaaaa tgatcgagca
     8221 gaaagcgctt cagcccttcg ccataggaca catcgagcca gccgttcggc acgacaaagc
     8281 ccagacggcc accctcgcgc aagaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnncggga tcgagcgtga agaagttggc acgttcgatc accgcgccgg gaatgatcgt
     8461 cgccgccaat gccgcctcgg ggtccagttc aatgccatac agaacacccc cttcttccgg
     8521 gcgatcggcg gccagccatg agcgccatag cgccgcccgc cgcagcagcg ccccatcgcc
     8581 gcaaccggga tcgagcagtc gatcatccga acggcgcagg caaaagccga gcaacaagtc
     8641 ggcgacatca atgggtgtga caaattgtcc ccaggcgcgc tgtttgctgg tcatggaact
     8701 ccgggcaggc gacaatcggt gaaaaagccg tcaagggcga ttatactata agtgatatga
     8761 ccgcaatatc ccagaagacg ttcttcgata ctctgcccga tgcactgcgg ccgtcgctac
     8821 cggtggcact acagggcttt caggtgcggc ggccgtgggg tctgctgcag atccacttcg
     8881 gtgaaccggc cttgcattat gaggtcagct acgtaggtca gcgcctcggc cggctcatca
     8941 ccggctggga gcttggcttt cattgtgagg cgcgcgacca ccacctgaac cggctattgc
     9001 tcgacggctt tagtcatcac ctgtttgaga tcaaggctac gttgggagag catgtggaag
     9061 cggagatgtg ggatcgcggc tggacgaaaa tctacgaggt ttatcccgac gcgccgctga
     9121 cgcaggccta tcgcgcggac gtagccactc ggctggcgga gttcatcacc tgcatccacc
     9181 ccatcttcgt cggtctgcgg cgctcggcgg ccgagatata tcgataagtg gccggtagcg
     9241 ggtgatattt cccactcacc cgctaccggc cgaatctgct acttcttgat gatgatcggc
     9301 agcaatgagt attgcatacc gccatcgcta aacgttgccg tcacctgttt cggcccgttg
     9361 atcaggaacg aaatggttgg ctgcgtgccg gtcaccgcgc cactccaatt gctgaaggtc
     9421 gcgcccagac caggattggc cgttagggta acattggcgc cgcaagtcaa ttgaccaatc
     9481 ggcgggttcg cggtcactga accactgcca actatcgtta tatccagcag cggccgctcc
     9541 gcagacattg gcccaagggt tgcgttgtgg aagtaatcaa caacagctgt atgcgccggc
     9601 gcgggagagt tctttccttg tacattaccc gcgaagatac cggccttggt cacattcagc
     9661 gtatatttgt tgaacgaacc tgcctcgatc catgtggcac cgttgccgct gtactccatc
     9721 ttccaaattt ccccggtgcg cgtgacacgc agatagaccg catcagccgg attgacgata
     9781 tcgactgcta tacgcgcttt cgacttggtt ccttcaatgg tcgccgcgta ggctttaaga
     9841 ccattattat attgaatttc aaatcgggcg aaattttgcg catctccctc aacaatgatg
     9901 ccttgcattt gggcagtttg ggttacggtc gagtcgaatt tgacgacgta ttggaaatca
     9961 acattatcgg ccgcctgcat cacccgcgga gcatccttgt tatcacccca gagattatgc
    10021 gtcgcaccct ccggcacggt gatgcgca
//
LOCUS       DAOMQG010000099        10034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2899_length_10034_cov_10.053412, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000099 DAOMQG010000000
VERSION     DAOMQG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_10034_cov_10.053412"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /gene="menE"
                     /locus_tag="PLC06_07325"
     CDS             complement(<1..1484)
                     /gene="menE"
                     /locus_tag="PLC06_07325"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HRO90293.1"
                     /translation="MHEFGLTTDWLRARTLASPEALAVITEGSAYTFRELDAMVSRLC
                     GRLRQEGVVAGEHIAILMPNSLAAVCAIFAMGRLGTIAVPLNTRLTQGEIVWQMKRAD
                     CNRMLCSPTTEPVATEVAAATDLPLHVFPETPAEWEAWLAPLPMSPDATLSNQDLSSL
                     QAIQFTSGTTGFPKGAMITYANHLWGAIGSAFKLGVQANDRWLVCMPLFHVGGMAILF
                     RSCLYGTAIVLQRGFDEAAVLHSLAAEKITLISLVPTMLKRLLDANLNVDRAPDLRLI
                     LLGGAAAPASLLESAFDAKLPVAVTYGLTETASQVATMRPTEARAKPGSSGRPLLFSE
                     IKTVDENGTMTAANQPGEVAVRGPSVMAGYYADEAATHATLHDGWLYTGDIGYLDDDD
                     DLWILNRRSDLIVSGGENIYPAEIERVLRVNPHVVEASVVGLPHPLWGQQVAALVQLD
                     RPAAIDENQLLSYCRQSLAGYKQPRLLAFIDEMPMTGSGKIHRHA"
     gene            complement(1512..2342)
                     /gene="menB"
                     /locus_tag="PLC06_07330"
     CDS             complement(1512..2342)
                     /gene="menB"
                     /locus_tag="PLC06_07330"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRO90294.1"
                     /translation="MTDWQVVKEYTDITYHKADGMARIAFNRPESRNAFRPLTIDEMI
                     DAFRDVWMDPEVGVVLLTGNGPAKDGKYAFCSGGDQRVRGHAGYIGDDGVARLNVLEL
                     QRIMRTMPKPVIALVAGYAVGGGHVLHVVCDLTIAAENAIFGQSGPKVGSFDGGFGSN
                     YLASIVGQKKAREIWYLCRQYSADEALQMGLVNKVVPLEQLEAEGVQWATEILEKSPI
                     AIRFLKAGLNADLDGQTGLQVLAGDATMLYYMTEEGREGKEAFLEKRKPDFQKYPWRP
                     "
     gene            complement(2393..>3786)
                     /locus_tag="PLC06_07335"
     CDS             complement(2393..>3786)
                     /locus_tag="PLC06_07335"
                     /inference="COORDINATES: protein motif:HMM:NF027896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRO90295.1"
                     /translation="RWSVEVPTPDIGSPDVTLRHLRTLAARACATAGGLTMGPVHLNF
                     PFRKPLEPDPSESGKGEQETSATGFIPRAHTLISRGRLTPDEQQISEIAALIAEYPRG
                     LIICGPECPGDNFPAAVTALAERSGYPILADPLSNLRHGPHIANGFVLGGQHLWLPSI
                     TEAMARPDIILRFGSVPTSAVLAGYLGRITPVAHIHIRENGEWADDSHLTSRLIQADP
                     STLCSLVEGRLGDHGYQADGCWAASFKEIEGQTWERVTEALQAAPFFDGSAIRQTLTS
                     LPERSIVFVGNSMPIRHVDTFDQPSTRSITFFGNRGASGIDGNVSTALGVAAATGQPV
                     TAILGDITFYHDMNGLLAVGQHDLQNITFVVVNNDGGGIFYRLPVAAHEPPFTDLFLT
                     PHGLTFEHAAAMYGLRYATVADAAELEAELKVDAGTARIIEIRTNSAADYSQMRAIST
                     AVTTGLETIFTQS"
     assembly_gap    3787..3886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3887..4332)
                     /locus_tag="PLC06_07340"
     CDS             complement(<3887..4332)
                     /locus_tag="PLC06_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRO90296.1"
                     /translation="MDLSTNINTRWMAIFVDELARNGLDAVCIAPGSRSTPLTLAFAA
                     QPAIRIYRHLDERSAGFFALGMAKATRKPVALVCTSGTAAANFHPAIIEAYYSHVPLI
                     ILTADRPAELRGSGANQTIDQIKMYGDHVRWSVEVPTPDIGSPDVTL"
     gene            complement(4323..5705)
                     /locus_tag="PLC06_07345"
     CDS             complement(4323..5705)
                     /locus_tag="PLC06_07345"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HRO90297.1"
                     /translation="MNIELFLRGSFGQERFYWSEPDSAEQPMTLAGLGIAAEVSVPPV
                     LDNTGEEPTLPGHRFSAIARKASDLFGEALVLPVDWKVDAGIDENWRQHPARPRLFGG
                     FAFQDDFIPDNTWSVFSPAQFILPHYQFIQIDGDKFLTINALAAWDESIAGCHAGLKE
                     ALLARLALVLPEDEGNNSAIDINYPMSADTWAKMIGHATHSIKDGELEKVVLSRVCEI
                     RTEQLIDAAATLPYLNHNYNDCYRFFFEPIPFNAFFGATPELLIRKESGDIITMALAG
                     SIARGQSPDEDKALARQLLTSEKNRHEHQLVVDTINNSLRDVTTELRFPSEPGVLRLR
                     NIQHLLTPVSGRLGDTETSVLTLVRLLHPTPAMGGVPAGVALPYLRLLEPVPRGWYAA
                     PIGWFDAYGDGEFAVAIRSAVSQYARAWLYAGAGIVGDSDAEQEWAETALKFRPMLGA
                     LDVKESAPWI"
     gene            complement(5870..6502)
                     /locus_tag="PLC06_07350"
     CDS             complement(5870..6502)
                     /locus_tag="PLC06_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRO90298.1"
                     /translation="MADSDKTTPLRDPGSTKARILDAALSTFSRKGYHDTRLDDIVDE
                     SETSKGAIYFHFANKERLFLALVDQFADLLERRVLDAIDGKAEGMARVQIALETVLDT
                     FGRYRRPAKILLVQAVGLGNIFENKRAEVNDRFARLIEGYLKEAVAIGDIEQVDTEVV
                     AYAWMGAIYGIVIRWVTTGEPEPARIMNSLVPLLLRSVGYEAATEAAARP"
     gene            6745..7176
                     /locus_tag="PLC06_07355"
     CDS             6745..7176
                     /locus_tag="PLC06_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRO90299.1"
                     /translation="MNKNILILLVIGALLLSLAACGIDDVLPNNSPSDEPTPIGNVEN
                     GEALFAKPTIGANNAPGCITCHSLEADVVIVGPSQAGLATRAESRVPGQSAEDYIRAS
                     ITDPNAFVVEGFAEGMMYQNYGTDLTAAEINDLVAFSLTLK"
     gene            complement(7238..8020)
                     /locus_tag="PLC06_07360"
     CDS             complement(7238..8020)
                     /locus_tag="PLC06_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO90300.1"
                     /translation="MSRQQYAMGDGAVISCLDEGQGEPLLFIHGFTGTAIADHAGLMD
                     EFRPTHRLIGPDLRGYGASRPPNRDFPVDFYQRDAGDMAALLDRINCGPVTVLGFSDG
                     AEVSLLLAAMRPDLVRGVVAWGVCGVISSEEVLAVQSWLPLTNWDETRPEWKRQIIER
                     HGAEQFESMITGWLDAASGIYERGGDISYNLSNRIQCPVLIMNGDGEVGNTPRDVRRL
                     AKRIPNCRLEFIADSGHPIHKDQPELFNAKVRGFVEGLKESP"
     gene            complement(8032..9666)
                     /locus_tag="PLC06_07365"
     CDS             complement(8032..9666)
                     /locus_tag="PLC06_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRO90301.1"
                     /translation="MSELGSQVNPNSADYKANYAGNKAMAEQLAKRQQLVASDRSARV
                     IDLQRQRGKLLTRERIEALIDPGGAFLELSSLAAWDMYEGEAPGAGIVTGIGRISGVD
                     CMIIANDPTVKGGTYFPETVKKHLRAQAIAEENHLTTIYLVDSGGAFLHLQADVFPDR
                     DHFGRIFYNMARMSAAGITQIAAVLGSCTAGGAYIPAMADETVIVKDNGTIFLAGPPL
                     VKAATGEEVTAEELGGGDVHTRVSGVADHYSETEPEALDKVREIVSYLQPGLALRPHT
                     IPLATQMPEEPLYPADELYGIIPVDPRQTYDVREVIARLVDGSRLSEFKARFGTSIVC
                     GFAHIMGIPVGIVANNGLLFSESALKATHFIQLCGQRGTPLLFMQNITGFMVGKQYEN
                     EGIAKHGAKMVMAVSNVNVPRITLLHGVSHGAGNYGMSGRAYDPRFLFSWPNSRISVM
                     SGDSAADTLWMVGKKQVLRQLDNPSESDIQQAEVKFKRPILDQYNYESDPYFATARLW
                     DDGILDPVMTRTALGLAFATTGNAPLPTAGYGTFRM"
     gene            complement(9672..>10034)
                     /locus_tag="PLC06_07370"
     CDS             complement(9672..>10034)
                     /locus_tag="PLC06_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRO90302.1"
                     /translation="TYRLTGKQGVDVVVDNVGAATFQSSLRALKRGGRLLTVGNSSGP
                     HIEIDNRFIFGKHLSIIGSTMGTSVDYETVMGLIFQGKLRPIIDSVYPLEEGGKALQR
                     LSEGDVVGKLVLRIRQEN"
BASE COUNT         2008 a         2844 c         2922 g         2160 t
ORIGIN      
        1 gcatgacgat gaattttccc cgatcccgtc atcggcatct cgtcgatgaa tgccagcaaa
       61 cgcggctgtt tgtacccggc cagggattgg cggcaataac tcagcaattg attttcatcg
      121 atcgccgcgg gcctgtccaa ttgcaccaga gcggccactt gttggcccca gagtggatgc
      181 ggcagcccga cgacactggc ctcaaccaca tgggggttaa ctcgcaggac tcgttctatc
      241 tcggccggat aaatattctc gccgccactg acgatcagat cgctgcggcg attgaggatc
      301 cacaagtcat cgtcgtcgtc cagatagccg atatcgcctg tatagagcca gccatcatgc
      361 agcgtcgcat gagtggccgc ctcgtccgca tagtagccgg ccattaccga agggccgcga
      421 acggcgacct ctcccggctg gttggcggcg gtcatcgtcc cgttttcgtc gacggtcttg
      481 atctctgaga acaaaagcgg ccgaccgctg ctgcccggct tggcgcgcgc ttcggttggc
      541 cgcatggtgg ctacctgcga ggccgtttcc gtcaatccat aggtgacggc cacaggcagc
      601 tttgcgtcaa atgcactttc cagcagactt gcgggagcgg ccgcgccgcc aaggagaatt
      661 agccttaaat ccggcgcacg atcgacattc aaattcgcgt cgagcagtcg cttcaacatg
      721 gttggcacca gagagatcag cgtgatcttc tcggccgcca ggctgtgaag aaccgctgct
      781 tcatcaaagc cccgttgcag aacgatagca gtcccgtata ggcaagagcg aaacaggatt
      841 gccatgccgc ccacatggaa cagcggcatg caaaccagcc agcggtcgtt ggcctggacg
      901 cccaatttga aagcggaacc tatcgccccc catagatggt tggcgtatgt aatcatagcc
      961 cctttgggaa agccggtggt gcccgaagtg aactgaatag cttgcaggga actgaggtct
     1021 tgatttgaga gtgtggcgtc aggagacatc ggcaatgggg ccagccatgc ctcccattca
     1081 gccggcgttt cgggaaaaac atgcaagggc aagtctgtcg ctgccgcaac ttcggttgcc
     1141 acgggttcgg tggtcggcga acagagcatg cgattgcaat cggctcgctt catttgccag
     1201 acaatttcac cctgggttag ccgcgtattg agcgggacgg cgatcgttcc caatcgaccc
     1261 atggcgaaga tggcgcacac ggcggcaagt gaattcggca tcaggatcgc gatgtgttct
     1321 cccgccacga cgccttcctg cctcaggcgg ccgcagagcc gggagaccat cgcgtcaagc
     1381 tccctgaagg tataggccga accttccgtg atgaccgcca gggcttcggg cgaagccagc
     1441 gtccgcgccc gaagccagtc agtcgtcaat ccaaactcat gcatagtttg ttttcggcaa
     1501 gagaagaagt attagggtcg ccagggatac ttctggaagt ccggcttgcg cttctccagg
     1561 aaggcttcct tcccttcgcg cccttcttcg gtcatgtagt agagcattgt ggcgtcgccg
     1621 gccagcactt gcagcccggt ttggccgtcg agatcggcgt tgagtccggc tttcaggaac
     1681 cggatggcga tggggctttt ctccaggatt tccgtggccc attgcacgcc ttccgcctcc
     1741 agctgttcga gcgggacaac cttgttgacc agccccattt gcagcgcttc atcggcggaa
     1801 tattgccggc acaaatacca tatctctcgc gccttcttct ggccgacgat ggatgccagg
     1861 taattggagc cgaagccgcc atcgaagctg cccaccttcg gtcctgattg gccaaagatg
     1921 gcgttttccg cggcgatggt cagatcgcaa acgacgtgga gcacgtgacc gccaccgacg
     1981 gcgtacccgg ccaccagggc gatcaccggc ttgggcatgg tgcgcatgat gcgctgcagt
     2041 tccagcacgt tcaggcgagc aacaccgtca tcgccgatat atccggcatg gccgcgcacc
     2101 cgttgatccc cgccggagca gaaggcatac ttgccatcct tggccgggcc attgccggtc
     2161 agcaatacaa cgccaacttc ggggtccatc catacgtcgc gaaacgcatc gatcatttcg
     2221 tcgatcgtca gcggtcgaaa tgcgttgcgc gattccgggc gattaaaggc aatgcgggcc
     2281 atcccgtcgg ccttgtggta ggtaatgtct gtgtactctt taacgacttg ccaatctgtc
     2341 atggaatagt tctcctatgt tttcttgtgt gcgtcatcgt cgccgggtcg agtcacgatt
     2401 gggtgaagat tgtttccaag ccggtagtga cggcggtcga gatggcgcgc atttgggaat
     2461 aatctgcagc gctgttggtc ctgatttcaa tgattcgagc agtgcctgcg tcgactttca
     2521 attctgcttc cagttcggcc gcatcagcga cggtcgcgta acgtaggcca tacatggcgg
     2581 ccgcatgttc gaaggttaaa ccgtgcgggg tcaggaacag gtcggtaaat ggcggctcgt
     2641 gagcggccac cgggagccga tagaatatcc cgccgccatc attattgacc acgacaaagg
     2701 tgatattttg cagatcgtgt tgcccgacgg ccaacaagcc gttcatgtca tgataaaaag
     2761 tgatgtcacc gagtatggcg gtcaccggtt gccccgtggc ggcggcaacc cctaacgccg
     2821 ttgagacgtt gccgtcaatg ccgctcgccc ctcgattgcc gaagaacgtg atcgaccgcg
     2881 tcgatggttg gtcgaaggta tcgacgtggc gaataggcat gctgttgccg acaaatacga
     2941 tcgacctttc cggtagtgaa gtgagcgttt gcctgatagc cgatccatcg aagaacgggg
     3001 cggcttgcag tgcctcggtg acgcgctccc aggtttggcc ttctatctcc ttgaagctcg
     3061 cggcccaaca gccatcggcc tgatagccat ggtcgcccag gcgcccttcg accagcgaac
     3121 ataatgtgga cgggtcggcc tggatgaggc ggctcgtgag gtgcgaatca tcggcccact
     3181 cgccgttctc acgaatgtgg atatgagcga ccggcgtgat acgacccaaa tatccggcaa
     3241 gaacggccga cgtcggcact gagccaaacc ttagaatgat atcgggccgc gccattgcct
     3301 ccgtaataga agggagccat aggtgctgcc cccccagcac aaagccgttc gcgatgtggg
     3361 ggccatggcg taaattcgac aacggatcgg ccaggatcgg ataccccgaa cgctcggcga
     3421 gggccgtgac tgcggcggga aaattatcac cggggcattc cggcccacag atgatcaggc
     3481 cgcgcggata ttcggcgatc agggcagcga tctccgagat ctgttgttca tcaggtgtca
     3541 ggcggccgcg gctaatgagc gtgtgcgcgc gtgggataaa accggttgcg ctggtttcct
     3601 gttccccttt tccactttct gacgggtctg gctccagcgg cttgcggaag gggaagttta
     3661 gatgcaccgg ccccatcgtc agaccgccgg ccgtggcgca ggctctggcc gccagcgtgc
     3721 gcagatggcg aagcgtgacg tcggggctgc caatatcggg ggtcggcact tcgacagacc
     3781 agcgcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcg tgacgtcggg
     3901 gctgccaata tcgggggtcg gcacttcgac agaccagcgc acatggtctc cgtacatttt
     3961 gatctggtca atggtttgat tcgcgccact gccacgcaac tcggccggcc gatcggcggt
     4021 cagaatgatc aatgggacgt gggagtagta agcctcgatg atggccgggt ggaaattcgc
     4081 ggcggccgtg ccggaggtgc agaccaatgc gacgggcttt cgagtggcct tggccatgcc
     4141 cagagcgaag aacccggcgc tgcgttcgtc caggtggcga tagatgcgaa tggcaggctg
     4201 tgcggcaaag gccagcgtga ggggcgttga gcgagaaccc ggggcgatgc atacggcatc
     4261 cagtccattg cgagcgagtt cgtcgacaaa gatcgccatc cagcgggtgt ttatgttcgt
     4321 gctcagatcc atggggcact ttccttaacg tcaagcgcgc ccagcattgg ccggaacttc
     4381 agcgccgttt cggcccactc ctgctcggcg tctgaatcgc ccacaattcc cgccccggca
     4441 tataaccagg cgcgcgcgta ttgcgagacg gccgagcgaa tcgccacggc gaactcgccg
     4501 tcaccgtatg cgtcgaacca gccaattggc gcggcatacc agccccgtgg tacaggctca
     4561 agcaggcgca aatacggcaa cgccacaccc gccggaacgc cgcccatggc gggggtcggg
     4621 tgcagcaggc gaaccagggt cagcactgat gtttcagtat cgcccagccg gccacttacg
     4681 ggtgtcagca ggtgttgaat gttgcgcaac ctgagcacgc ccggttccga cggaaaccgg
     4741 agttcggtcg tcacatcgcg gaggctgtta ttaattgtat ccacgacgag ctgatgttcg
     4801 tggcggtttt tctctgaagt gaggagttgt cgggcgagtg ccttatcttc atcgggggat
     4861 tgtccgcggg cgatcgaccc cgccaaagcc atggtgatga tatcgccgga ttccttgcga
     4921 atgagcagtt ccggcgttgc gccgaagaaa gcgttgaagg gaatcggctc gaagaaaaag
     4981 cgatagcagt cgttatagtt gtgatttaga tagggcaggg tggccgccgc atcaattagc
     5041 tgttcggttc ttatctcgca gacccgtgat agaactactt tttcaagctc tccgtctttt
     5101 attgagtggg tagcatggcc gatcatcttc gcccatgtgt cggccgacat cggataattg
     5161 atatcgattg ctgagttatt cccctcatct tcggggagga caagcgccag ccgtgccagc
     5221 agggcttctt tcaatcctgc gtggcaaccg gcgatacttt catcccatgc tgcgagggca
     5281 ttgatcgtca ggaacttatc gccatctatt tgaataaact gatagtgggg cagaataaat
     5341 tgagcagggc tgaagacgga ccaggtgtta tcggggataa agtcatcctg gaaggcgaac
     5401 ccgccgaaaa gccgcggccg agccgggtgc tgtcgccaat tttcgtcgat accggcatca
     5461 actttccaat ccacaggtag aacgagcgcc tcgccaaaca ggtcgctggc cttccgggcg
     5521 atggctgaga agcgatggcc gggcagtgtt ggttcttcgc ctgtgttgtc caggacaggg
     5581 ggcacgctaa cttcggcggc tattcccagc ccggccaacg tcatcggctg ttcggccgaa
     5641 tcaggttcag accaatagaa acgctcctgc ccgaagctgc cacgcaaaaa tagctcgata
     5701 ttcatttgag gcgcgggagc aaacaggctg actaatcgat atggagcgtc gtcccgttta
     5761 gcggcttgat cccattccgc gacatcattc gcatggggtg tccggatttc actccgtggc
     5821 gcgggtgagt gaatggcgtt cattgaggtt atctcgaata ccctcttgct cacggccgcg
     5881 ctgcggcttc ggtggctgct tcatacccca cgctgcgtag tagcaagggc acgaggctgt
     5941 tcatgatgcg ggcgggttcg ggttcgccgg ttgtcaccca gcggatgaca atgccgtaga
     6001 ttgcccccat ccaggcataa gcgacgactt cggtgtcgac ttgttcgata tcgccaatcg
     6061 cgacagcctc tttcaaatag ccttcaatca agcgggcaaa gcgatcattg acttcggctc
     6121 gcttgttctc gaagatattg cccaggccga cggcttgaac cagcaagatc ttggccggcc
     6181 gccgatagcg gccgaatgta tcgagcacag tctctagggc aatctgaacg cgagccatcc
     6241 cctcggcctt cccgtcgatc gcatccagca ctcgccgctc cagcaagtct gcgaactggt
     6301 ccaccaatgc caggaacagc ctttccttgt tggcgaagtg gaagtagatc gctcctttag
     6361 aggtctcgga ttcgtccact atgtcgtcga ggcgtgtgtc gtgatatccc ttgcgcgaga
     6421 aggtcgataa agcggcatcg agaatgcgcg cctttgtgga gcctgggtca cgaagcgggg
     6481 tggttttatc tgaatcagcc atggttgaat gctcgcgcgg cggttgtcat atcgacaaac
     6541 cgaccggtca gtctattatt ctagcaaacg ttgatacgtc tgcccatctt ttatcgctcc
     6601 ttgtaatgct tcgtaaacgt attttggcga tcacaatgta tcgccccgtt gggtttggcg
     6661 cgctcaccgg tagcgcatag aatcggaata gttgatgctg agcgaggatt gtcgatggct
     6721 cggcgacagg gtgttcaatt gcttatgaat aagaatatat tgatcttgtt ggtgataggt
     6781 gcgttgcttc tgtctttggc agcatgcggc atcgatgatg tcttgcccaa taattctccg
     6841 tccgatgagc caaccccgat tggtaacgtg gagaatgggg aagcgttgtt cgcgaagccg
     6901 accatcggcg ccaataatgc gccggggtgc atcacgtgcc attcgctgga ggcagatgtc
     6961 gtcatcgtgg gaccgtcgca agccgggttg gccactcgcg ctgaaagccg tgtgcccggt
     7021 caatcggccg aggattatat tcgcgcctca attacggatc cgaacgcgtt tgtcgtggaa
     7081 gggtttgccg aggggatgat gtatcagaat tacggcactg atttaacggc cgcggagatc
     7141 aatgatctgg tggcctttag cttgaccctt aagtagtgtg gcggtttgga taaggcaacg
     7201 gcagagtgac aaaaagcggg attgaggccc cactttttta tggcgattct ttcaatccct
     7261 caacaaagcc ccgaaccttg gcattgaaca actccggctg gtctttgtga attgggtgcc
     7321 cactatcggc aatgaattcg agccggcagt tcgggattcg cttggcgaga cgtcgcacat
     7381 cgcgtggtgt gttgcccacc tcaccgtcgc cgttcatgat gagaaccggg cactgtattc
     7441 gattggacag gttgtaagag atatccccgc cgcgctcata tatcccgctc gcggcatcga
     7501 gccaaccggt aatcattgac tcaaattgtt ccgcgccatg ccgctcgatg atctgtcgct
     7561 tccactcagg gcgcgtctca tcccaattcg tcagtggcag ccaggattga acggccagca
     7621 cttcttctga tgaaatcacc ccgcacaccc cccaggcgac cacgccgcgc acgaggtccg
     7681 ggcgcatggc ggccaacagc aacgatacct cggccccgtc gctgaacccc aggacggtga
     7741 ccggtccgca attaattctg tcgagtaggg cggccatgtc acctgcgtca cgctgataaa
     7801 aatcgacggg gaaatcgcga ttcggtggcc ggctggcccc gtagccacgc aaatccggcc
     7861 cgatcaggcg gtgcgttggt cggaattcat ccattaatcc ggcatggtcg gctatagcag
     7921 tgccggtgaa tccgtggatg aagagtaacg gttcaccctg cccctcatcg agacaggaga
     7981 tgacagcgcc atcgcccatt gcgtactgtt gtctgctcat cgactgaatc cttacatgcg
     8041 gaaggttcca tagcctgccg tcggaagcgg cgcattacct gtcgtggcga acgccaatcc
     8101 cagcgcggtg cgcgtcatga ccggatcaag gatgccgtcg tcccacagcc gcgccgtggc
     8161 gaaatagggg tcactctcgt agttatattg atcgagaatc ggccgcttga atttgacttc
     8221 cgcttgttgt atatcgcttt cggaagggtt gtccagttgc cgcagaacct gcttcttgcc
     8281 taccatccat aaagtgtcgg ccgcggagtc accgctcatc acgctgatgc ggctgttggg
     8341 ccaggagaac agaaagcgcg ggtcataggc ccggccgctc atgccgtaat tccctgcgcc
     8401 gtggcttacg ccgtgcagca aggtgattcg cggaacgttg acgttactga cggccatcac
     8461 catcttggcc ccgtgtttgg cgatgccttc gttctcgtat tgcttaccga ccatgaagcc
     8521 ggtgatgttc tgcatgaaca gtaacggcgt ccctcgttgg ccacagagtt gaatgaagtg
     8581 agtcgccttc agcgcggatt cgctaaacaa tagcccgttg ttggctacga tgccgaccgg
     8641 gatgcccatg atatgggcga acccgcaaac gatcgatgtg ccgaaacgag ccttaaactc
     8701 gctcagccgg gaaccgtcaa ccagtcgggc aatcacctca cgcacgtcgt aagtctggcg
     8761 cgggtcgaca gggatgatgc cgtaaagctc atcggccgga tagagcggct cttcgggcat
     8821 ttgcgtagca agcgggatag tgtgcggccg cagggcaagg cccggctgga gatagctgac
     8881 tatctcgcgc accttgtcca gcgcctcagg ttcagtctcg ctgtaatgat cggccacgcc
     8941 gctcactcgc gtgtgaacgt cacctccgcc cagttcctcg gccgtcacct cttcgccggt
     9001 cgcagctttc accagcgggg ggccggccag gaagatcgtc ccattgtctt taacgatcac
     9061 cgtttcgtca gccatggcgg ggatataggc cccaccggcc gtgcatgagc cgagaaccgc
     9121 ggcgatctgg gtgatacccg ccgccgacat gcgcgccatg ttgtagaaaa tacggccgaa
     9181 atgatcgcgg tcggggaaca cgtccgcttg tagatggaga aatgcgccac ctgaatcgac
     9241 caggtagatc gtcgtcagat ggttttcttc ggcgatcgcc tgagcgcgca aatgtttctt
     9301 gaccgtttca gggaagtagg tgccgccttt aacggtggga tcgttggcga tgatcatgca
     9361 gtcgacgccg gatattcgcc caatgccggt tacgatgccc gcgccggggg cttcgccctc
     9421 gtacatatcc caggctgcca acgacgataa ttccagaaac gcgcctccgg gatcgatgag
     9481 agcttcgatc cgttcgcgcg tcagcaactt gccccgttgt cgctgcaaat caataacgcg
     9541 cgccgatcga tccgatgcta ccaactgttg ccgtttcgcc agttgctcgg ccatcgcctt
     9601 attgccagca taattagcct tatagtctgc gctatttgga ttgacttgac tgcccaattc
     9661 cgacatatga tttaattctc ctgacggatg cggagcacga gtttgccgac gacatcccct
     9721 tcgctcagcc gttgtagcgc cttgccgcct tcttccaacg gatagacaga gtcgatgatc
     9781 ggccgtagct tgccttggaa gatcagcccc atgaccgtct cataatcaac gctggtcccc
     9841 atcgtgctac cgatgatcga taaatgcttg ccgaatataa accggttgtc aatctcaata
     9901 tgggggccgc tggagttacc cacggtcagt aatcggccgc ctcgtttcaa tgcgcgcaag
     9961 gagctttgaa atgtggctgc gccgacgttg tccacgacga catcgacccc ttgcttgcct
    10021 gtcagtcgat aggt
//
LOCUS       DAOMQG010000100         9913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2937_length_9913_cov_7.239805, whole genome shotgun sequence.
ACCESSION   DAOMQG010000100 DAOMQG010000000
VERSION     DAOMQG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2937_length_9913_cov_7.239805"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..858)
                     /locus_tag="PLC06_07375"
     CDS             complement(421..858)
                     /locus_tag="PLC06_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRO90303.1"
                     /translation="MNGDDRELILKDEVFRIVGAALEVSNELGAGFLEAVYQEALDIE
                     LRSRAIPFVAHPLIRIAYKGRYLVKEYIPDFLCFDQVIVEIKAMKELTNMEQAQILNY
                     LKASGKPVGLLINFGSPRLEWKRMVFTKPSIRRGPLMDANDRK"
     gene            1410..>1981
                     /locus_tag="PLC06_07380"
     CDS             1410..>1981
                     /locus_tag="PLC06_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRO90304.1"
                     /translation="MIPVEICIEASEPTAVAHAAGAAYHGGAATVELCAAMALDGLTP
                     SPDCLAAARQAFRQRPGLMAMIRPRAGGFGYAPDELALMAAQIDAAARDGADGVVFGV
                     LRAADQRIDSAANQRLLAAAHERGLKATFHRAFDATPDPDTALDTLIELGFDRVLTSG
                     LPWGQPGTALDGATRLARTILRAGDRIEIVA"
     assembly_gap    1982..2016
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2209..4119
                     /locus_tag="PLC06_07385"
     CDS             2209..4119
                     /locus_tag="PLC06_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRO90305.1"
                     /translation="MRRGPIAAGTGYHSMFTSLRNLLIGSPLPSSKSHDTRLDKVRAL
                     ASLSPDALASVAYANQEIYLGLLVAGAAGLLHSLDLALVITGLLGILALSYMQTIAAY
                     PSGGGSYTVAKENLGVLPGLIAAAALLMDYILNAAVSLTAGVAAIASAFPALWPYRVA
                     LSLVLLVFITVINLRGLKEAGTAMIVPVYLFLVVYLGMIAFGIYVALVDGPGTFPVDI
                     PVPAETGPLGALAPVTIFLLLRTFASGATALTGVEAISNAVPIFKPEETVNARKTMVA
                     MAVLMGILFVGTIGLTEYLAVVATADETILSALARRLFGSGLIYYLVQAATLLVLIVA
                     ANTSFTDFPRVASLMARDGFMPRQLRTLGDRLVFSNGIVLLAALAGALIVIFQGDTHL
                     LIPLFAVGAFLAFTLSQSGMVVHWWRERGRHWHAKAFMNGLGALATGIALLVIGASKF
                     AHGAWIVILLIPLLVLMFRSIHKHYDDVGRQLRIEGEIPQVGCTYHTHLAMPVAGIHR
                     GVLEAINFACSIAKDVTAIYVELEPGTGEAMRERWQAAGLDKVANLVTVPSPYRSLIG
                     PFLDEIDRLDAERDDDRAVSVLIPEFIPAKWWHFLLHNQTALPLKWALLYRRHRHDKA
                     RVVIDMPFYLDR"
     gene            4370..7057
                     /locus_tag="PLC06_07390"
     CDS             4370..7057
                     /locus_tag="PLC06_07390"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRO90306.1"
                     /translation="MPVPILVTKLYAPPPSPKAVPRPRLIERLDKGLAAGHKLTLVSA
                     PAGFGKTTLVSEWAAGCGRPAVWLSLDEADNDPARFLAYLIAALQTVATDVGAGASAA
                     LQSPQPPAVESILTVLLNEIAAMPDGFILVLDDYHLIDSKAIDHTLTFLLDHLPPPVH
                     LVIATREDPSLPLARLRARGQLTELRAADLQFTPAEAAEFLNRVMGLALSDGDVAALE
                     TRTEGWIAGLQLAALSMQGRDDVAGFIEAFTGSHRFVLDYLVEEVLKQQPERIQIFLL
                     RTSILARMCGPLCDAVLLDPSVPGQETLERIEHANLFLVPLDSERRWYRYHHLFGDLL
                     RRRLGKPPELADYHLRACEWHEANGGLAEAFHHALAAGDFERAAGLAEAAWPEMERNF
                     QTTVWLGWVEELPNAVVGSRPGLCVTLARAWTDAGEVDKSETWLQNAERALARETLTD
                     KSKTLPATIALIRGDNAQNVGNLAETLRYTELALQLAPEEDMYLHAHAAITLGFTQWA
                     TGDLEASLRALYAWMEDMEEMGNREFAIASVFAMADMLVILGRLGEADKALRQAIRRA
                     EAQGPEAEAVTAHHHLGLALLAHERGDDAAAARHFESAAELGRRTTLIDWHYRWNLAQ
                     ARLRESEGAWDAALDLLDEAGQTYVKNPIPILRPIEAHKARVYLKQGRPDMAVAWTRS
                     RGLAVSDEVTYPGEYEYLTLARVRLAEGNFAGVDELLERLLTAAEAQKRTGSVIEILL
                     TRALVHQARNDLPQALAALERALAAAEPEGYVHILVGEGDAMRRLLLKFRSVIEKQTA
                     HPLKGYAGKLLAAFPSPIETGSQTAITKQGDGLIEPLTNRELEILHLIAEGHSNAEIG
                     RRLFLALSTVKGHNLRIFGKLQAQNRTEAVARARELGLL"
     gene            7132..7377
                     /locus_tag="PLC06_07395"
     CDS             7132..7377
                     /locus_tag="PLC06_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90307.1"
                     /translation="MKPTQAQPTSYEIRVAGHLPPHWADWFEGLTITLEEDGDTLLTG
                     PVIDQAALHGLLKKVRDLGLSLVSVCPVEAEPPAAQL"
     gene            7386..7571
                     /locus_tag="PLC06_07400"
     CDS             7386..7571
                     /locus_tag="PLC06_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90308.1"
                     /translation="MNVKRLSVEFVTVFAIALVTTAMVTLLWNIVGHGAGVIDWETSL
                     CFALIFGVILTWVKSRE"
     gene            7734..8186
                     /locus_tag="PLC06_07405"
     CDS             7734..8186
                     /locus_tag="PLC06_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006507922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HRO90309.1"
                     /translation="MNTIATPAKKIETKTLLSTLWVVVMIDMLKADILSLNIPGAAEE
                     LARTAGDTPIPLLMLGGAVMMEVSTLMILLSRVLPYRVNRWANIIVSLITIAFIVAGG
                     ASYPHYLFIAAVEVVCLLLIVWFAWKWRAPAEEYAPRPDRSAGETAII"
     gene            8269..8994
                     /locus_tag="PLC06_07410"
     CDS             8269..8994
                     /locus_tag="PLC06_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HRO90310.1"
                     /translation="MKTLQKSGGYAALYMAIAYLIGLILFIVVLDYPGITDPAQKVAL
                     LVEKPMVIFATNLFMYVFFGVSLVVLSLALYDRLKSGAPAMMQAATALGIIWAGSLIA
                     AGMVSNAGIEPTIALHATDPTQAALSWQGIETVAAGLGNGNGEILGGLFTLLVSLAAL
                     RGGALPRWLNILGLLVGAVGILTINPRLADLTGVFGLGQMVWYVWLGIALLRDGKVTV
                     TAGSRPVGRPDGEAGGDAAAATR"
     gene            9472..>9913
                     /locus_tag="PLC06_07415"
     CDS             9472..>9913
                     /locus_tag="PLC06_07415"
                     /inference="COORDINATES: protein motif:HMM:NF022022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HRO90311.1"
                     /translation="MNDMTITVIDQRGVDFYGDELTAVRASDGQIYVSVRHLCNALEL
                     NQRPQVLRIQRNSILADGYQGEIKMITPGGLQRLGMLRVDLIPLWLAGVSLQAVKESS
                     RPKLERFQREAAKVLWEAFQQGRLTAELDFDSLLAQDTPEAQAYK"
BASE COUNT         1899 a         3125 c         2880 g         1974 t
ORIGIN      
        1 gaatgggagc catgattcac cagcagcaat ccaattgttt gttcggtcat aaagctaaac
       61 actcctgtgg ttgtatgata aaccgggcct catgggagaa acccggcttc cggtactcgg
      121 cgggaaaccg gcctccggtt ctccgcttat aaatcttgag tataggttct gtcagagttg
      181 ggattgtact aaaaaaccgg gggtttttac aagccaatta ataaaaatac aagccggcac
      241 gtcacgacac ggttcaaggg ttgggtaatc tgacctgccc ccaaaacccg gccccaacac
      301 ttactctcct tcattcgcgt tcattcgcgt ccattcgtgg cttctctcct ggttctccgc
      361 aaatgaacgc gaatgaacgc gaatggggaa tttggcaaag atcataagtg gtatctctct
      421 ttatttgcgg tcattcgcgt ccattagcgg ccctctgcga atggacggtt tggtgaagac
      481 catgcgtttc cattccagcc ggggcgatcc gaaattgatg agcagcccga ccggcttccc
      541 gctcgctttc agatagttga gaatctgtgc ctgctccata ttggtcaact ccttcatcgc
      601 cttgatttcg acgatgacct gatcgaaaca aaggaaatca gggatgtact ccttgaccag
      661 atagcgaccc ttgtaagcga tccgaatcag ggggtgagca acgaacggaa tggcgcggct
      721 tctcaactca atgtcgagcg cctcctgata gaccgcctcc agaaacccgg cccccagttc
      781 attcgacacc tccagcgccg cccccacgat cctgaagacc tcatccttca aaatcaactc
      841 gcgatcgtcc ccgttcattt ccaggcctcc tccactaaaa aatattccca tccattgata
      901 cccacccaac ttcattcgcg cccaacatcc ccattcgcgc ccattcgcgt ccattagcgg
      961 tccccctcct ggttatccgc aaatgaacac gaatgaacgc aaatgggaga tttggcaaag
     1021 atcataggtg gtcccttctt ctattcgcgc ccattcgcgt ccattagcgg cttctctcct
     1081 ggttctccac aaatgaacac tgatgaacgt gaaagggaaa tttggcaaag atcataggtg
     1141 ccccttcttt tattcgcgtc cattcgcgtc cattagcggc ttctctcctg gttctccaca
     1201 aatgaacact gatgaacgtg aaagggaaat ttggcaaaga tcataggtgc cccttctttt
     1261 attcgcgtcc attcgcgtcc attagcggtc ccataggtgg aaaaagtgta gcattgcggc
     1321 cgacgccggg caaacgggct ggggagaaag ggagcagggg cgatcgtcca tgacgcctcc
     1381 actgtgctag aatggagccg gagcatcctg tgataccggt tgagatttgt atcgaagcat
     1441 ccgaacccac ggccgtggcg catgccgccg gcgcggctta ccatggcggc gcggccaccg
     1501 tcgaactatg cgcagccatg gctctggacg gcctgacccc ctcgcccgac tgcctggcgg
     1561 ccgctcgcca agccttccgc caacgacccg gtctgatggc catgattcgc ccccgcgcgg
     1621 gcggtttcgg ctacgcgccg gatgagctgg cgctgatggc agcgcagatc gacgcggccg
     1681 caagagatgg cgcggacggc gtcgtcttcg gcgtgttgcg cgccgccgac cagcggatcg
     1741 attcggccgc caaccagcgg ctgctggccg cggcccacga gcgcggcttg aaagccacct
     1801 tccaccgcgc cttcgacgcc acgccggatc ccgacaccgc cctggacacg ctgatcgagc
     1861 tgggcttcga ccgcgtgctg accagcggcc tgccctgggg ccaaccgggc acagcgctcg
     1921 acggcgcgac ccgactggcg cggaccatcc tccgcgccgg ggatcgcatc gagatagtgg
     1981 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgg agccgctgct ggctcggctg
     2041 ccgcttggcg ccgggcgggt gtcgatccac gcctactccg gcgcacaaat cggcggccgg
     2101 accgcgatcg aagcggtacg cgcgctcgtg gtggcggcag acgtgaaggg cggatagggt
     2161 gaaggggcaa acgcctcttg ccaatccgtc gattttccct atagtaagat gcgccgcggc
     2221 cccatcgccg ccggcacagg gtatcattct atgttcacct cattgcgtaa tctgctcatc
     2281 ggttcccccc tgccgtccag caagtcccac gacacgcggc tggacaaggt gcgcgcgctg
     2341 gcctcgctct cgcccgacgc gctggcctcc gtggcctatg ccaatcagga gatctacctc
     2401 ggcctgctgg tggccggggc ggccgggctg ctccactcgc tcgatttggc gctggtcatc
     2461 accggcttgc tgggaatcct ggccctttcc tacatgcaga ccatcgccgc ctacccgtcc
     2521 ggcggcggct cctatactgt cgccaaggag aacctgggcg tgctgccggg gctaatcgcc
     2581 gcggccgcgc tgctgatgga ctacatcctc aacgccgcag tcagcctgac ggccggcgtg
     2641 gcggccatcg cctccgcctt ccccgccctc tggccctatc gcgtggcgct gtcgctggtt
     2701 ctgctggtgt tcatcaccgt gatcaacctg cgcggcctca aggaagcggg cacggcgatg
     2761 atcgtccccg tctatctgtt cctggtcgtc tatctgggca tgatcgcctt cggcatctat
     2821 gtggcgctgg tcgacgggcc ggggacgttc ccggtcgaca tccccgtgcc ggccgagacc
     2881 gggccgctgg gggcgctggc tcccgtcacc atcttcctgc tgctgcgcac cttcgcctcc
     2941 ggcgccacgg ccctgaccgg cgtggaagcc atcagcaacg ccgtgcccat cttcaagcct
     3001 gaggagacgg tcaatgcccg caagacgatg gtcgccatgg ccgtcctgat gggcatcctg
     3061 ttcgtcggta caattggcct gacggagtat ctggccgtgg tggccacggc cgacgagacg
     3121 atcctgtccg cattggcccg gcggctgttc ggctccggcc tgatctacta tctcgttcag
     3181 gcggccacgc tgctcgtcct catcgtggcg gccaacacca gcttcaccga tttcccgcgc
     3241 gtggcctcgc tcatggcccg cgacgggttt atgccgcggc agctgcgcac gctgggtgac
     3301 cggctggtgt tctccaacgg catcgtgctg ctggcggcgc tggccggggc gctgatcgtc
     3361 atcttccagg gcgacaccca cttgctcatc ccgctgttcg ccgtgggcgc gttcctggcc
     3421 ttcaccctgt cgcagagcgg catggtcgtc cactggtggc gcgagcgcgg ccgccattgg
     3481 cacgccaagg cattcatgaa cggcctcggc gcgctagcga cgggcatcgc cctgctcgtc
     3541 atcggcgcca gcaagttcgc ccatggggcg tggatcgtca tcctgctcat ccccctgctg
     3601 gtgctcatgt tccgcagcat ccacaagcat tacgacgacg tcggccgcca actgcgcatt
     3661 gagggagaga taccccaggt cggctgcacc tatcacaccc acctcgccat gcccgtggcc
     3721 gggattcatc gcggcgtgct ggaggcgatc aacttcgcct gctccatcgc caaggacgtg
     3781 accgccatct acgtcgagct ggagccgggc acgggcgagg ccatgcgcga gcgctggcag
     3841 gccgccgggc tggacaaggt ggccaatctg gtcaccgtgc cgtcccccta ccgctcgctc
     3901 atcggcccgt tcctcgacga gatcgaccgc ctcgacgccg agcgggacga cgaccgggcc
     3961 gtcagcgtcc tcatccccga attcatcccg gccaaatggt ggcatttcct gctgcacaac
     4021 cagacggcgc tgccgctcaa gtgggcgctc ctctatcgcc gccaccgcca cgacaaggct
     4081 cgcgtggtca tcgatatgcc gttttatctg gatcgatgag agcagggacg gcgctttgca
     4141 tcgaagccag accaaaatag ttatcagcgg cagcgcgtgg ccggcgctaa aataaggagt
     4201 ggttgagttt tactcaaacc ttcgcaatcc tgtcggttac ggagcggcgg attcgagtgc
     4261 ggcggctgcc gttataaatt ttggctgttg aagtggcatg ttgcaaaacc gggcgtgccc
     4321 tgcctcaact gactgcccac tattccgggc atgcgattat aattaattca tgcccgtccc
     4381 aatccttgtt accaaactct acgccccccc accgtcgccg aaagcagtcc cccgcccgcg
     4441 cctgatcgag cgactggaca aggggctggc ggcgggccac aaactgaccc ttgtctccgc
     4501 tcccgccggc ttcggcaaga cgacgctggt cagcgagtgg gccgccggtt gcggccggcc
     4561 ggcagtctgg ctgtcgctgg acgaagcgga taatgacccg gcccgctttc tggcgtacct
     4621 catcgcggcg ttgcagaccg tcgcgaccga cgtcggggcg ggggcgtcgg ccgcgctcca
     4681 atccccgcaa ccgcccgccg tcgagtcgat tctgaccgtc ctgctcaatg agatcgccgc
     4741 catgccggac ggattcatcc tggtgctcga tgactaccac ctcatcgact ccaaagccat
     4801 cgaccacacc ctcacctttc tgctcgatca tctgccgccg ccggtgcacc tggtgatcgc
     4861 cacgcgcgag gacccgtcgc tgccgctggc ccggctgcgg gcgcgcggcc aactgaccga
     4921 gctgcgcgcc gccgacctgc aattcacccc ggccgaagcg gccgagtttc ttaatcgagt
     4981 aatgggtctg gcgctttccg acggcgacgt ggctgcgctg gaaacccgca ccgagggctg
     5041 gatcgccggg ctgcaactgg ccgcactctc catgcaaggc cgggacgacg tcgccggttt
     5101 tatcgaggcc ttcaccggca gccaccgctt tgtgctcgat tatctggttg aggaggtctt
     5161 gaaacaacag ccggaacgga ttcagatatt cctgctgcgc acgtccattc tcgctcgcat
     5221 gtgcggcccc ctgtgcgatg ccgttctgct tgacccctct gttcccggac aggaaaccct
     5281 ggaacggatt gaacacgcca acctgtttct tgtcccattg gacagcgaac ggcgctggta
     5341 tcgctatcac catttgttcg gcgatttgct gcgccggcga ctcggcaagc cacctgagct
     5401 tgccgattac cacctgcgcg cctgcgagtg gcatgaggca aacggcggcc tggccgaagc
     5461 cttccaccat gccctggcgg ctggtgactt tgagcgcgcg gcgggtctgg ccgaggcggc
     5521 ctggccggaa atggagcgca atttccaaac taccgtctgg cttggctggg tggaagaact
     5581 gcccaatgcg gttgtgggtt ctcggcccgg tttgtgcgtc acgctggctc gcgcctggac
     5641 ggacgccggg gaagtggata agagtgaaac gtggctgcaa aacgccgaac gcgctctggc
     5701 gcgcgagacg ctgacggata aatcaaagac actgccggcg actattgccc tgattcgcgg
     5761 tgacaatgcc cagaacgttg gtaaccttgc cgagacgttg cgatataccg aactggccct
     5821 ccaactcgct cccgaggagg atatgtacct ccacgctcat gccgccatta ctctgggatt
     5881 tacccagtgg gccaccggcg acctggaagc ttccctccgc gccctgtacg cctggatgga
     5941 agacatggag gagatgggca accgggaatt tgcaatcgcc agcgtattcg ccatggccga
     6001 tatgctggtg atattgggtc gcctgggcga agctgacaaa gccctgcggc aggctatccg
     6061 gagggctgaa gcacaggggc cggaagcaga agcagtcacc gcccatcatc atctggggct
     6121 ggcgctgctg gcccacgaac ggggcgatga cgccgcggcc gcccggcatt ttgaatcggc
     6181 cgccgaactg ggccggcgca cgaccctgat cgattggcat taccgctgga atctggccca
     6241 ggcccggctc cgggaatccg aaggagcatg ggatgcggcg ctcgacttgc tcgacgaagc
     6301 cggccaaacc tacgtcaaaa acccgatccc catcctgcgg ccgatcgagg ctcacaaggc
     6361 gcgggtatac ctgaagcaag gacggccgga tatggccgtg gcctggacgc ggtcgcgcgg
     6421 cctggcggta agtgacgagg tgacctaccc aggcgagtac gaatacctca ccctggcacg
     6481 agtgcgattg gctgagggga atttcgccgg agtcgatgaa ttgctggaac gcctgctgac
     6541 ggcggccgaa gcgcaaaaac gaaccggaag cgtcatcgaa atcctgttga cccgggcgct
     6601 tgttcatcaa gcgcggaacg acctgcccca ggctcttgcc gcgctggagc gcgccctggc
     6661 agcggccgag ccggaggggt acgtgcacat cctggtaggt gagggggatg cgatgcgccg
     6721 gttgcttttg aaattcagat cagtgattga gaaacaaacc gctcatccgc tgaaaggcta
     6781 cgcgggcaaa cttctggccg ccttcccgtc gccgatagag accggttccc aaaccgcgat
     6841 tacaaaacag ggcgacggtc tcatcgaacc gctcaccaac cgcgaactcg aaatcctgca
     6901 tctcatcgcc gaaggacact ccaacgcgga gatcggccgg cggctattcc tggccctcag
     6961 cacggtgaag ggccataacc tgcggatatt cggcaaactc caggcccaaa atcgcaccga
     7021 ggccgtcgcc cgcgcccgcg agttgggtct gctctaatcc ctcgaaataa tactccgatc
     7081 aatactttag ttgctatccg gcagcaccgc cctgccggta tagtgacgat catgaagcca
     7141 acgcaagcgc agcccaccag ctacgaaatc cgggtcgcgg gccacctgcc gccgcattgg
     7201 gcggactggt tcgaaggcct gaccatcaca ctggaagagg acggcgacac gctgttgacc
     7261 ggcccagtga tcgatcaggc cgcgttgcat ggcctgctca aaaaagtgcg cgatctgggc
     7321 ctgtcgctgg tctcggtctg ccccgttgag gccgaaccac cggccgccca attgtaagga
     7381 gatttatgaa cgtcaagaga ctgtccgttg aatttgtaac cgtgttcgcg atagccctgg
     7441 tgacgacggc aatggtgaca ttgctatgga acatcgtcgg tcatggagcc ggtgtcatcg
     7501 attgggaaac ttcattgtgc tttgcgctca tcttcggcgt catcctgact tgggtgaagt
     7561 cgcgggaata attgcatacc actatcgggc gggatcaatc ccgccgctga tataccaaac
     7621 cggctcaagc cggttgccgc cgccgccaaa cctgtttcaa caggtttcgt ataacagcag
     7681 cggatttgaa tccgccctat ccatatcata tcgttccaaa acaggagaca acattgaaca
     7741 caattgcaac ccccgcaaaa aagatcgaaa cgaaaaccct gctctcgacc ttgtgggtcg
     7801 tggtcatgat cgacatgttg aaagccgaca tcctgtcgct caacatcccc ggcgcggcgg
     7861 aagaattggc aagaacagcc ggcgacacgc cgatcccgct gctgatgctg ggcggggcgg
     7921 tcatgatgga ggtttccacc ctgatgatcc tcctctcccg cgtcctgccg taccgggtga
     7981 atcgttgggc caacatcatt gtgagcctca tcaccatcgc gttcattgtc gccggcgggg
     8041 cctcataccc ccattacctg ttcattgccg ctgtcgaagt agtctgcctg ctgctgatcg
     8101 tctggttcgc ctggaaatgg cgcgccccgg ctgaggagta tgcgcccaga cccgacaggt
     8161 cggcgggtga aacagcgatc atttaggaca tcaatcacct gcccgtcagg ccttattgga
     8221 aagtcaattt gtcaacccta tcacagtcgt tcaaaaaagg agccaatcat gaaaacctta
     8281 cagaaatccg gcggctatgc cgcgctatac atggcaatcg cctatctcat cgggctgatt
     8341 ctcttcatcg tcgtgctgga ctaccccggc atcaccgatc cggcccaaaa ggtggccctg
     8401 ctcgtcgaaa agccgatggt cattttcgcg accaatctgt tcatgtacgt gttcttcggc
     8461 gtgtcccttg ttgtgctgtc gctggcgctc tacgaccggc tcaagtccgg cgcgccggcg
     8521 atgatgcagg cggcgaccgc gctggggatc atctgggccg gttcgctgat cgccgccggc
     8581 atggtctcca atgccgggat tgagccgacc atcgccctcc atgccaccga cccgacccag
     8641 gccgcgctga gctggcaggg aattgaaacc gtggccgccg ggctgggcaa cggtaacggt
     8701 gaaatcctgg gcggtctctt cacgctgctg gtcagtctgg ccgcgctgcg gggcggcgcg
     8761 ctccccaggt ggctgaatat cctcggtttg ctggttgggg cggtgggcat cctcaccatt
     8821 aacccaaggt tggccgactt gacgggagta ttcggcctgg gccagatggt ctggtacgtc
     8881 tggctgggaa tcgcgctgct gcgcgacggc aaggtcactg tcaccgccgg cagccggccc
     8941 gtcggcaggc cggacggaga agcgggcggc gatgcggccg ccgcgaccag gtaatcctca
     9001 agccaaccca ttggggaaaa ggggcaggag acaccaacct gccccttttt tgcgaaaacc
     9061 ttcccatccg gcttataatt gttgcagtga cgcaacaggg tgacagggga tgtcataatg
     9121 gcactccctt cattacaagg aaggctcatg atgacccctc ctaatggcat gtgaattaca
     9181 tactattcca acagatagcg aaaggagtag ccaaatcggc cactccttca agccaatcgg
     9241 gtgtcatggc gactcgccat tgatgacagg ttcaccgtac tgctttgtat tttcggcccg
     9301 ggtttgtatg agtaacacac aagcctcttg ggattgtcac aatcgaaatt gagggatata
     9361 gatggaaaac cgggcgctaa ttcccatcaa tcaaaggggt gatcattttg atcacccctt
     9421 accggcgaca aatgtcagtt tgcggtttta gcggtgtaaa ggctggtatt tatgaacgac
     9481 atgacaatca cagtgatcga ccagcgtggt gttgattttt atggcgatga actaacggcc
     9541 gttcgcgcca gtgatgggca aatctacgtt tcagttcgac atttatgtaa cgccttggag
     9601 ctaaatcaac ggccgcaagt cttgcgcatc cagcgcaatt cgattctggc cgatggatac
     9661 caaggggaga tcaaaatgat cacccctggc ggtttgcaac gactaggtat gctcagagtg
     9721 gatctcatcc cattatggtt agctggtgtc agccttcagg ctgtcaaaga gagttcccgc
     9781 cccaagctgg aacgcttcca gcgcgaggcg gccaaagtgt tgtgggaggc gttccagcaa
     9841 ggccgcctga cggccgaact cgacttcgat tccctgctgg ctcaggacac gcccgaagcc
     9901 caggcctaca aga
//
LOCUS       DAOMQG010000101         9794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_2988_length_9794_cov_9.571825, whole genome shotgun sequence.
ACCESSION   DAOMQG010000101 DAOMQG010000000
VERSION     DAOMQG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_9794_cov_9.571825"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLC06_07420"
     CDS             complement(<1..995)
                     /locus_tag="PLC06_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90312.1"
                     /translation="MYDEQAPDLIDPDELSEEDLDSSSSRHGCLYLTITLVIIASLVS
                     SSILTYFVVTGYEIRTGTPAIGRDARVVESGPTAAPATVVAAAAATDPAAEAVTTAVA
                     ETAMPVFEPAAVAPEINRIAIVNADGQVETMSPSGEDRRALTLTGDNARFQFPTWSPD
                     GHRLALIGNRMLGGAIYVLDDAARSDGLRDHQVYYSAEEAPVYLFWSPDSRNLGFLAN
                     RDRNSLSLNVVAGDGAAESRELAIGAPFYWDWSDDGRQLLIHAGSRGAKHVLALIDVD
                     GRMQADNLAVPGNFQAPGIGPGGRYWAFAEEAGNGLTSLVVVDTQTGERSSHQQVG"
     gene            1098..1562
                     /locus_tag="PLC06_07425"
     CDS             1098..1562
                     /locus_tag="PLC06_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90313.1"
                     /translation="MSTQKSSVKENALSGFIQAAGVILAVSYPVLALSAGVRAFYQLF
                     LKEDVANYVPAILSGVAALAYLLATVGFVVRKRWAWRLSVAALAFELVMVLLIGTLSF
                     VIPDTIGRTVWGHFGADYGYFPLIQPLLGLAWLFHPQTRKVYRIGRAAVETA"
     gene            complement(1598..2560)
                     /gene="glpX"
                     /locus_tag="PLC06_07430"
     CDS             complement(1598..2560)
                     /gene="glpX"
                     /locus_tag="PLC06_07430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRO90314.1"
                     /translation="MDNDLTSRNIGLDLTRVTEAAALAAGRWIGSGNFDAAHAAATEA
                     MYNMLVQLGVSGCVAIGEDRLIDGNAALCEGVTFGERPTCDLAVDPIDGTRLLIEGKS
                     GAVSVIAVAPRHSIWSAGPAEYLDKIVVDRDAAHVLVPECMDAPAAWTLALIARAKKK
                     SVRDLVVIVLQRLRHHDLIEEIRATGARIYLRQEGDTEGALEAAMPDTGVDVLMGVGG
                     ASQGVLSACAVRALRGGMLARLAPQSAEERTAVTQAGMDLRKIYTVDELVTSDEIFFA
                     ATGLTGTPILPGIAYKGNHAETHSLLIRAKTKTRRHIEAEYSLE"
     gene            complement(2560..4767)
                     /locus_tag="PLC06_07435"
     CDS             complement(2560..4767)
                     /locus_tag="PLC06_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRO90315.1"
                     /translation="MATVNKWSPRSTILPVLRYLQQPVTLFREYDRANLRPDLIAGLT
                     VGVILLPQAIAFALLAGLPPRMGLYTAIVGGSVAALWGSSDQLHSGPTNTISLLLLST
                     LALVATPGSPDFIVAASLIAVMVGVLQLALGLFRLGFIVNFVSHSVIVGFSAGAGFLI
                     AVRQIDPLLGLHVESDGVLSGLQGTFLALPETHLFTAALGAGTMLVIILLKRFAPRLP
                     GALIAIGLATLAVFLLGDLAKDVAVIGDLPSRIPPLAQLPLLDLNLIGRLSSGALAIA
                     AIGLVQTVAVSRSLAAQTRQRLDNNQEFVGQGLANIFSGLFSGYSVSGSFSVSAVKFK
                     SGARTRLASIVASLIVLIAMLTVGPVGAYMPVSALAGILIIAAFNMVDQAGIRRIAVG
                     ARGDAIIMIATFLGTIFLELDFAVLSGILLSFILYLIRTSAPRVQIVVPDENFQHFVR
                     RPGAPLCPQLGIVEIEGDLYFGAVSHIEEAILKLAAEHPEQRFLLIRMHHVNQVDFSG
                     IHMLESLIRTYRDKGGDVFLVRLSDAARRLMETTGCLNYLGSRNILDEDTAVEYLFQR
                     ILDPAVCIYECPVRVFRECQNLPKRFDLVDVSLPGNGAGAILGANGHESIPAIEPGEL
                     WRALRKPDPSEHPVVLDVREPREYRQSHIAEAQSLPLSTLLEKGLELVDDRPVVLVCR
                     TGRRSRRAAAILHNHGYRNVRLIAGGMQAWEAAGLLTAVEFDAEPDASGVASR"
     gene            complement(4884..5513)
                     /locus_tag="PLC06_07440"
     CDS             complement(4884..5513)
                     /locus_tag="PLC06_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRO90316.1"
                     /translation="MKILAVSDRVLDKLYSGQVSQSFPDIDLLIGCGDLPFYYLDFLT
                     SALNVPLLYVRGNHDNGPQYAADGGIWNDVRGGMDIHGKVIERKGLILAGLQGSMRYK
                     PHGEYMYSESEMRMVVAQMTPRLLWNRQRFGRALDVLVTHSPPYGIHDRPDLPHTGFK
                     VFLSFLRMFKPRYLLHGHVHVYRQDEVVNTHYGETTILNVYPYRVIDIP"
     gene            complement(5510..5809)
                     /locus_tag="PLC06_07445"
     CDS             complement(5510..5809)
                     /locus_tag="PLC06_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003734386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRO90317.1"
                     /translation="MKELEARMHAFVAEKGWYDSDSTKPQTPRNLAISLMLEAAEVLE
                     HFQWRDEARRPDELAGELADVALYLLQLASISGIDLEQAVLAKLERNRDREWPDP"
     gene            complement(5956..6975)
                     /gene="gap"
                     /locus_tag="PLC06_07450"
     CDS             complement(5956..6975)
                     /gene="gap"
                     /locus_tag="PLC06_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRO90318.1"
                     /translation="MTIKVGINGFGRIGRQVYKAIYENYKGVLDVEAINDLMPAETNA
                     HLLKYDSTYGKFPGEVEVRDGDIYVDGQKLKSYAEKDPAKLPWGELGVDIVLECTGRF
                     RDKATAGAHLTAGAKKVIISAPGGKDIDGTFVLGVNEETYDPATQNVISNASCTTNAL
                     APVAKVLDDSFGIKRALMTTIHAMTNDQSILDVAHKDLRRARTTANNIIPTSTGAAKA
                     IGLVLPDLKGKFNGMAFRVPTVTVSVVDLTAELEKPATKEEINAALKKASEEKGWLGK
                     VLNYSDEPLVSMDLKGDPASSTVDGLSTDVIDGNFIKVVTWYDNEWGYASRLADLTAY
                     VAEKL"
     gene            complement(7184..8419)
                     /locus_tag="PLC06_07455"
     CDS             complement(7184..8419)
                     /locus_tag="PLC06_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRO90319.1"
                     /translation="MLLALGAAIMAVSWLNLLLALSHADWVPAGIFGVLTLQFLPLWL
                     RVLLPLVVGGLVMLLAVIKLGKNLVEPLRAGPGAVVDSLYDYRLRDRGPHIVAIGGGT
                     GMPGLLRGLTAYTSNITAIVTVADDGGSSGRLRKELGLLPPGDFRNNLAALASDESLM
                     IQVLQYRFGTQIQTNGHEASTGQSLSGHAFGNLLLAALTGITGSFDEALLAAQRVLAL
                     RGQVLPSTLEDVTLVADVALAGEGVIEVVGESAIPEAGGRIEHVRLEPPDVHAYPPAL
                     RAIFQADLIVLGPGSLYTSILPNLLVGDLAEALRHCRAKVAYICNLATQPGETADYTV
                     ADHVDAIRQHVPGDLLDVVVANDNFSIPADTGGGNTQFVPVGPVQGIHMVACDLVDEA
                     RPWRHDSKKLARAAINLLN"
     gene            complement(8525..9037)
                     /locus_tag="PLC06_07460"
     CDS             complement(8525..9037)
                     /locus_tag="PLC06_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO90320.1"
                     /translation="MNDELNPKKDHLLDQIVNAWMDLQSVVSGLSESDLTRVNPDSGW
                     RVADHLFHLAAWEQGIVYLLTGRLRMDGMGVTPEQWRDLEMDQINDIIFERGRGRSAA
                     EAVTTLRKAHEEMLTALAPLNDADLQRGYLTFDTQVENDDRPIIGRIIGNTYEHYEEH
                     IGYIRAALAD"
     gene            complement(9054..9497)
                     /locus_tag="PLC06_07465"
     CDS             complement(9054..9497)
                     /locus_tag="PLC06_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO90321.1"
                     /translation="MLEQLSTMHRSLEKAIAGLSDEALDWSPGPEMNSLGVLMAHTLG
                     ATRFWIGDVVGGDPSGRVREAEFMTAGMSAAELIAHSHEVLAHSQSILSRLSLADLDA
                     IRKVPDSGREVTGAWAILHALEHMALHVGHMEITRQLWDAHQAGR"
BASE COUNT         2198 a         2986 c         2687 g         1923 t
ORIGIN      
        1 cccacttgct gatgggatga tcgctctccg gtctgtgtat ccaccacgac caaagacgta
       61 aggccgttgc cggcttcttc ggcgaacgcc caataacgcc cacctgggcc aatgccgggc
      121 gcctgaaaat ttccgggaac ggccaggttg tcggcctgca tccgaccatc aacatcgatc
      181 agggcaagca catgttttgc gccgcgagag ccggcatgga tcagcagttg gcggccgtca
      241 tccgaccaat cccaatagaa cggcgcccca attgccagtt cccggctctc ggccgccccg
      301 tctccggcaa cgacgttcag actcaacgag ttgcgatccc gattggcgag gaacccaaga
      361 ttccggctgt ccggcgacca aaagagatac accggcgcct cctccgcgct gtaatacacc
      421 tgatgatcgc gcaaaccgtc ggagcgggcg gcatcatcca gcacgtagat agctccgccc
      481 aacattcggt tgcctattaa ggccaaacga tggccatcgg gcgaccatgt cgggaactgg
      541 aagcgtgcat tatcacctgt cagagttaat gcccgacggt cttctcccga cggcgacatc
      601 gtctcaacct ggccgtcggc gttgacgata gcaatacgat taatctcagg cgccacggca
      661 gccggctcaa ataccggcat cgcggtctct gcaacggctg ttgtcacagc ttctgctgcg
      721 ggatccgtgg ccgctgcagc ggccacgacc gtcgcgggcg cggccgtggg gccgctctca
      781 actacgcgag catcccgccc tatcgcaggt gtcccggttc gaatctcata accggtcacg
      841 acaaaataag tcagaattga ggaggaaacg aggctggcga taatgacgag cgttatcgtc
      901 agatagaggc aaccatgtcg ggaagatgac gagtccagat cctcttcgct cagctcatcc
      961 ggatcgatta ggtcgggggc ctgctcatca tacataggac gattgtaaca tcacgaaggg
     1021 cggcgctcta gtcggcttac caattattta ataaccaatc gtgatcaaga gagaagacgc
     1081 gtggtaaaat gatgatcatg agtactcaga aaagctccgt caaggaaaac gcactgtccg
     1141 gcttcatcca ggccgcgggt gtcatattag ccgtctcata tccggttctt gcgctttccg
     1201 ccggcgtgcg cgcattctat cagttgttcc tgaaggaaga cgtggccaac tacgtgccgg
     1261 ccatactgag cggtgttgcc gccctggcct acttgctggc gacagtcggc ttcgtcgtgc
     1321 gcaagcgttg ggcatggcgt ctatcagtcg ccgcgctggc ctttgagctg gtcatggtct
     1381 tgctcatcgg cacgctcagc tttgtcatcc ccgacaccat cggccgcacg gtttggggac
     1441 acttcggcgc ggattacggc tacttcccgt tgatccagcc gttactgggt ctggcctggc
     1501 tcttccaccc gcaaacgcga aaggtctatc gcatcggccg cgcggccgtc gagaccgcat
     1561 aaatcagtca atcaccctca ccggaaaaac tctctgctta ctccagcgaa tactcggcct
     1621 cgatatggcg tcgggtcttc gtttttgccc gaatgagcag ggaatgggtt tccgcatggt
     1681 tacctttata ggcgataccc ggcaggatgg gcgtgccggt taagcccgta gcggcaaaga
     1741 atatctcgtc gctggtaacc aactcatcga ctgtgtagat cttcctcaaa tccatgccgg
     1801 cttgagtgac ggccgtacgt tcctcggccg actgcggggc cagccgggcc agcatgccgc
     1861 cacgcaacgc gcgaacagcg caagccgaca acaccccctg cgacgcccca ccgacaccca
     1921 tcagcacatc cacccccgtg tcgggcatgg cggcctcaag cgctccctcg gtatcacctt
     1981 cctgacgcag atagatccgt gccccggtgg cccgtatttc ctcgatcaga tcatgatggc
     2041 gcagccgctg caggacgatg accacgagat cgcgcacgga tttcttcttg gctcgagcaa
     2101 tgagagccaa agtccaggcg gccggcgcgt ccatacattc cggcacaagc acgtgcgccg
     2161 catcccgatc gacgacgatc ttgtccagat actccgccgg ccccgccgac catatgctat
     2221 ggcgcggagc gacggcaatg accgacaccg cgcctgactt tccctcgatc aacagccggg
     2281 tgccgtcaat cgggtccaca gccagatcgc aagtcggccg ttcgccgaag gtcacaccct
     2341 cgcacagagc cgcgttgccg tcgatcaggc gatcctcgcc aatcgccacg cagccgctga
     2401 cgcccagctg cacgagcatg ttatacatgg cctcggttgc ggccgcgtgg gccgcatcga
     2461 agttgcccga accaatccag cgcccagccg cgagcgcggc cgcttcggtg acgcgcgtca
     2521 gatcaaggcc aatattacgc gaggtaaggt cgttatccat catctcgatg ctactccgct
     2581 tgcatcaggt tccgcgtcga actcgaccgc cgtcaacaat ccggcagcct cccaggcctg
     2641 catgccgccg gcgatcaggc ggacattgcg atagccatgg ttgtggagga tagccgccgc
     2701 gcgacggctg cgtctccccg tccggcacac caacacgacg ggtctatcat ccaccagctc
     2761 caatcctttc tccaacaatg ttgaaagtgg caaggactgg gcctcagcga tatgggattg
     2821 gcgatattcg cggggttcgc gaacatcaag gacgacagga tgttcggacg gatccggttt
     2881 gcgtaacgct ctccaaagct ctcccggctc gatggccgga atgctctcat gtccattcgc
     2941 ccccagtatg gcgccagccc catttcccgg cagcgataca tcgaccaaat caaaccgttt
     3001 gggcaagttt tggcactcac gaaaaacacg aaccgggcat tcataaatgc acaccgccgg
     3061 atccagaatg cgctgaaaca gatactcaac ggccgtgtcc tcgtccagga tattccgtga
     3121 tcccaggtag ttcaggcagc ccgtcgtttc catcaggcgg cgcgccgcat cgctcaaccg
     3181 taccagaaaa acatccccgc ctttgtcgcg ataggtgcgg atcaggcttt ccaacatatg
     3241 gataccgctg aaatcgacct gattgacatg atgcatccta ataagcagga atcgttgctc
     3301 aggatgttcg gccgccagct tcaggatggc ctcctcaata tggctgaccg caccgaaata
     3361 caagtcgccc tcgatctcga caatacccaa ttgcgggcaa agtggcgcgc cgggacgtcg
     3421 gacgaaatgc tgaaagttct cgtccggcac gacaatctgg actcgcggcg cgctggtgcg
     3481 aatgagataa aggatgaagg agagtagtat gccgctgagg acggcgaaat caagctccag
     3541 aaatatcgtg cccaggaagg tggcgatcat aatgatggca tcccccctgg cccctacggc
     3601 aatacgccga attcccgcct gatcaaccat attgaaggca gcaataatca aaatgcccgc
     3661 caaagcgctg accggcatgt aagcgcccac aggaccaacg gtcaacatcg ctatcaggac
     3721 gatcaggctg gccacgattg acgccaatcg cgtcctcgcc cctgacttaa acttcactgc
     3781 cgaaacggag aaggagcctg aaaccgagta accgctaaac aagccggaaa agatattggc
     3841 caggccttga ccgacaaatt cctggttgtt gtccagacgc tggcgcgttt gggcggccaa
     3901 cgatctggaa acagccaccg tctggaccag tccaatcgcg gcaatcgcca acgcgccgga
     3961 ggagaggcgg ccgatcaggt tcagatccag caagggtaat tgcgcgaggg gtggtattcg
     4021 gctgggcaga tcaccaatga cggcgacatc cttcgcgagg tcaccgagca gaaacacggc
     4081 cagcgttgcc aggccaatcg caatgagcgc tcccggcagg cgcggagcga atcgctttaa
     4141 gagaatgatc accagcattg tgccggcgcc aagcgcggcc gtgaacagat gcgtttcggg
     4201 tagtgccagg aatgtgcctt gcaaaccaga gaggacgcca tcactctcaa catgtaaccc
     4261 cagcagtgga tcaatttgac ggacagcgat gaggaacccg gctcctgccg aaaatccgac
     4321 gatcaccgaa tgggaaacga aattgacaat aaaccccagc cgaaacaaac ctaacgccag
     4381 ctggaggacg ccgaccatca cggcgatcaa actggcggcc acgataaagt cgggcgagcc
     4441 aggcgttgca acgagcgcca atgtcgataa caacaataat gaaattgtgt tagtaggccc
     4501 ggaatgaagc tgatcggatg acccccacag cgcggcaacc gagccgccaa cgatggctgt
     4561 gtagaggccc atccggggtg gtaacccggc cagcagcgcg aacgcaatgg cctgaggtag
     4621 tagaatcacc ccaacggtca gcccggcgat caggtcgggc cgcaggttgg cgcgatcata
     4681 ttcgcgaaac agcgtgacgg gctgttgcaa atagcgaagg acaggcagga tcgtactgcg
     4741 aggcgaccac ttattcactg tggccacagc cgcgccgcct ccacgtgaaa gcctgccgtc
     4801 atcggggacg gggggtgggt gaacgggttg atcgtgcaac gatggcattc tatgtgaatc
     4861 aaccgtgtcc ggcaattaag ccattaggga atatcgatta ctctgtaggg gtaaacattg
     4921 aggatcgttg tctccccata atgagtgttg acgacttcat cctgccggta gacatgaaca
     4981 tgcccgtgca ggagatagcg cggcttaaac atccgcaaaa agctgaggaa gaccttgaag
     5041 ccggtatggg gcaggtcggg ccgatcatga atcccgtagg ggggtgaatg ggtcacgagg
     5101 acatcgagcg cgcggccgaa tcgctgccgg ttccacaaca ggcgcggggt catctgcgcc
     5161 acgaccatgc gcatctccga ttccgagtac atgtattcgc cgtggggttt atagcgcatc
     5221 gagccttgta agccggccag gataagtccc ttgcgctcga tgaccttgcc gtggatatcc
     5281 atcccgccgc gcacatcgtt ccaaatcccc ccatcggccg catactgcgg tccgttatcg
     5341 tgattgccgc gcacgtacag gagcggcacg ttcagcgccg aggtaaggaa atcaaggtag
     5401 tagaaaggca ggtcgccaca accaatgagg agatcgatat ccggaaacga ttgactcacc
     5461 tgaccactat agagcttgtc caggacgcgg tcgctgacgg ccaatatttt catgggtcag
     5521 gccattctcg atcccggttc cgttccagct tggccaggac agcctgctcc agatcgatgc
     5581 cgctgatgct cgccagttgc aacagataga gcgcaacgtc agccaattcc ccggccagtt
     5641 cgtcaggcct ccgggcctcg tcgcgccact gaaaatgttc cagcacctcg gccgcctcca
     5701 gcatcaaaga tatggcaagg ttgcgcggcg tctggggctt ggtcgaatca gaatcatacc
     5761 agcccttctc cgccacgaac gcgtgcatcc gtgcttccag ctctttcaac tccataggtt
     5821 gctgatttta accaaataat gaggccgccg gccaccttaa taaaggagac cggcggcctt
     5881 gtcgatcaaa caggcacgtg cctcagatcg gcgagacaaa tcgtattagt cccgcctgtc
     5941 atagccatgc cagatctaga gcttttcggc gacgtaagca gtcaggtctg ccagacgcga
     6001 ggcatacccc cactcattat cataccaggt cacgaccttg atgaaattgc cgtcgatgac
     6061 gtcagtggac aatccatcaa ccgtcgaaga agccggatct cccttgagat ccatggaaac
     6121 gagcggctca tcgctgtaat taagcacctt gcccagccaa cccttctcct cagaggcctt
     6181 cttcagggca gcattaatct cttccttggt cgccggcttc tcaagctcgg ccgtcaggtc
     6241 aaccaccgat acggtgaccg tcggaacacg gaaagccata ccgttgaact tgcccttcag
     6301 atcgggcagc accaggccaa tagccttggc cgcaccggtc gaggtcggga tgatgttatt
     6361 ggccgtcgtg cgcgcgcggc gcaggtcctt atgggccacg tcgaggatgc tctggtcatt
     6421 ggtcatcgcg tgaatggtgg tcatcagggc gcgcttgatg ccgaaactat catcgagcac
     6481 cttggcgacc ggagccaggg cgttggtggt acagctggca ttggagatga cgttctgggt
     6541 cgcaggatcg taggtttcct cattgacgcc caggacgaac gtgccgtcga tgtccttgcc
     6601 gccgggagca gagatgatga ccttcttcgc gccggccgtc aggtgcgcgc cggcggtagc
     6661 cttgtcgcgg aagcggcccg tgcactccag gacgatatcg acgcccaact caccccaggg
     6721 cagcttcgcc gggtccttct ccgcatagct cttgagcttc tggccatcca cgtaaatgtc
     6781 gccgtcgcgg acctcgactt cgccggggaa cttcccatag gtggagtcat acttcagcag
     6841 gtgcgcattt gtctcggccg gcatcaggtc attgatcgcc tcgacgtcga gaacgccctt
     6901 gtagttctcg tagatcgctt tgtatacctg acggccgata cggccaaagc cgttgatgcc
     6961 aactttgata gtcatagata aatctccttt cgtagtgaaa tggtaattct ggtgttaagt
     7021 atgtggatca agtgggagtg cgctcactag aatcggaagt acactagtgg ggattatact
     7081 ctatctttcg tttgactgaa agtgtcggcg cgatctttgt gacatatgtc atgtatattc
     7141 acatgacagg ctacagagcc gcccaacgta acagatcaac ccatcaattc agcagattga
     7201 tcgctgcacg cgcgagcttc ttgctgtcat gccgccaagg tctcgcctcg tcaaccaggt
     7261 cacaggcgac catgtgtatt ccctggaccg ggccaaccgg cacaaattgc gtgtttcctc
     7321 cgccggtatc ggccgggatc gaaaagttat cattggccac gacgacatcc agcaaatcgc
     7381 cgggcacgtg ctgccgaatg gcgtcaacgt gatcggcaac cgtataatcg gccgtctcgc
     7441 ccggctgagt ggccaaatta caaatgtagg ccacctttgc ccggcagtgg cgtaacgcct
     7501 cggccagatc gccgaccagc aagttgggca gaatactggt gtacaggctg ccgggaccca
     7561 gcacgatcag atctgcctga aagatagcgc ggagcgccgg cggataggcg tggacatcag
     7621 gtggctcaag tcgtacatgc tcaatgcggc cgcccgcttc tggaatggcc gattcaccca
     7681 ccacttcgat gacaccttct cccgcaagag ccacatcggc caccaaggtc acatcctcca
     7741 gcgtcgacgg cagcacctgc ccgcgcaagg ccagaacgcg ctgagcggcc aggagtgcct
     7801 catcaaaact gccggtgatg ccggtcaaag cagccagcag caaattgccg aaggcgtggc
     7861 cgctcaggct ttgacctgtg gacgcttcgt gaccgtttgt ctggatctgc gtgccgaacc
     7921 ggtattgcag gacctgaatc atgagtgatt catcgctggc cagcgcggcc agattattgc
     7981 ggaaatcgcc cggcggcaac aggcccagct ccttgcgcaa tcggccgctg ctgccgccgt
     8041 cgtcggctac cgtgacgata gcagtgatgt tgctcgtgta agccgtcagc ccgcgcagca
     8101 agccgggcat gcccgtcccg ccgccgatgg cgacgatatg cgggccacgg tcgcgaagac
     8161 gataatcgta gagcgaatcc accaccgccc ccggcccggc acgcagcggc tcgaccaggt
     8221 ttttgcccag cttgatgacg gccaacaaca tcaccaggcc gccgacaaca agcggcagga
     8281 gcactcgcaa ccacagtggc aggaattgca gcgtcagtac gccgaagatg cccgcaggca
     8341 cccagtcggc atgactgagt gccaacaaca aatttagcca gctcaccgcc ataatagctg
     8401 cgccgagagc aagcaacatc aaccaccgct tgatgccgat gccgacagca aaccagcgac
     8461 gcctgatata aatcgaatcc cggagccgat gtcttcgagc cattcaatca caaataaaga
     8521 ccggctaatc ggccagtgct gcccgtatgt agccaatatg ttcttcgtaa tgctcatacg
     8581 tattcccaat gatccggccg atgatgggac ggtcatcatt ttcgacctgg gtatcgaagg
     8641 tcaagtagcc gcgctgcagg tcggcgtcat taagcggagc gagtgcggtg agcatctctt
     8701 catgagcctt gcgcagcgtt gtcacagctt cggcagctga acgaccacga ccgcgctcga
     8761 atatgatatc gttaatctgg tccatctcca gatcgcgcca ctgttcgggg gtgactccca
     8821 tcccatccat gcgcaatcga ccagtaagca gataaacgat accctgctcc caggccgcca
     8881 ggtggaacag atgatcggcc acccgccaac cggaatccgg gtttacccgc gttaaatcag
     8941 attcagacaa gcccgatacc accgactgga gatccatcca ggcattaaca atctgatcca
     9001 gcaaatgatc tttttttggg ttcaactcat cattcatctt agttacctct ttctcaccgg
     9061 ccggcttgat gcgcatccca caactgccgc gtgatctcca tatggccgac atgcagcgcc
     9121 atatgctcca gcgcgtgcaa aatggcccaa gcgccagtca cctctcgccc ggaatcaggt
     9181 actttgcgga tggcatccaa atcagcgagc gacaggcgcg acagaatgga ttggctatgc
     9241 gcaagaactt cgtggctatg ggcaatgagt tcggccgcgc tcatccctgc tgtcatgaat
     9301 tccgcttctc gcacccggcc ggatggatca ccgccaacca catcaccaat ccagaagcgg
     9361 gtcgcgccca acgtatgggc catcaatacg ccgagcgaat tcatctccgg ccccggcgac
     9421 caatccagcg cctcgtccga taagccggca attgcctttt ccaaagagcg atgcatggtc
     9481 gatagttgtt ccagcatctc ttgcagaatt atttccatct tgtctccata atatatgctc
     9541 cgacatcacc cggatgtcgt tcagaaatct ccacaccctt ccacaatgag ggactcagag
     9601 aaagcgatat aatcgatcat tatatcaggt aattagggat gaaagtggtc gtatggtggt
     9661 tgactcggtt caaagcaaaa atcgcgccta tctcgccctg gcgttcagtc ttattgctat
     9721 ggcattttca ggcattctgg tcagcctggc cggcgctccg ggcgcagtct cggccttcta
     9781 tcggatgtca atcg
//
LOCUS       DAOMQG010000102         9754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3002_length_9754_cov_12.719456, whole genome shotgun sequence.
ACCESSION   DAOMQG010000102 DAOMQG010000000
VERSION     DAOMQG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_9754_cov_12.719456"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="PLC06_07470"
     CDS             complement(<1..1579)
                     /locus_tag="PLC06_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRO90322.1"
                     /translation="MATFVEQVREMGIKQAIVTKADDVVERLTAGMNINDVRAALRGD
                     EPRRPNPRLRPHADGFWLHIRPSFYHTGVTNIYPTFRLGWLSTFMLVWETITGLILMV
                     FYTPSPLSAYGNMWNILGNVPLGQLFRNMHRLGAEVMVLVVAMHMLRTFVTGSYKKPR
                     QFTWATGMLLLVFTIFLSFSGYLLPWDQLAYWALTVFISGGAAAPAPAFVNENIMLIL
                     QGGPALGADGLLRWYLLHVLLLPLLAGIFFFVHYYKVVLYGISLPPGREQVGEDTAKR
                     VPKDERTYYIPDILTSELMWSALATLFLVAGALWLWDAPLETHADPVVTPLHVVAPWY
                     LSWSQGWLKLADKTLVVGFIPLLLVAFIVMPYIEVGKSRRYADRRVGLTVAMLFIAAM
                     LVSNWMGSPEFRVQSSPDREVSIQVLPEEGPGGIKGIPYEHLTPGTYSPGQVIDGNPF
                     LTRGLELFQSAMYQQSCTLTGNPQWTACTVETLDNGTRRFSNFFTTDAMPDPVAHLEI
                     VEVQPNLKELILHYEITN"
     gene            complement(1659..2315)
                     /locus_tag="PLC06_07475"
     CDS             complement(1659..2315)
                     /locus_tag="PLC06_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HRO90323.1"
                     /translation="MASQTATAESGAGASGGVSRREFLYYIWGASIALVSLQGAGLLV
                     WFLIPRFREGEFGGTFTVAAGDVPDINTPPVNYAEGRFWLVNLDSTQPNELMYQAPDE
                     PNPIQGIAAIYKVCTHLGCIYSWTPTNSRFECPCHGSKYRLDGRRVESPAPRTLDRFF
                     VQAIADDETTVLAEASLTDNFFQPLQLPSGTAFIRVDTGNRKQGPSQPLLCDFQGNCP
                     "
     gene            complement(2492..2565)
                     /locus_tag="PLC06_07480"
     tRNA            complement(2492..2565)
                     /locus_tag="PLC06_07480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2529..2531),aa:Val,seq:tac)
     gene            complement(2678..3232)
                     /gene="dcd"
                     /locus_tag="PLC06_07485"
     CDS             complement(2678..3232)
                     /gene="dcd"
                     /locus_tag="PLC06_07485"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRO90324.1"
                     /translation="MSVKPDHWIRRMVEEKNMIEPFIDGQVRDGVISYGLSSYGYDIR
                     VKNEFKIFTNVHSAIVDPKNFNPQSFVDYHGDTCVIPPNSFVLAQTVEYFRIPRDVIT
                     VCLGKSTYARCGLIVNVTPFEPEWEGYVTLEISNTTPLPARIYANEGIAQVLFFQADE
                     VCETSYADRKGKYQFQQSIVLPQI"
     gene            complement(3374..4138)
                     /locus_tag="PLC06_07490"
     CDS             complement(3374..4138)
                     /locus_tag="PLC06_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRO90325.1"
                     /translation="MNKTPVAAGEDGTKPVEGNELTGVRWVLTSYVNAAGEVVEALAD
                     REATAEFAADGRVAGNATCNNYFAGYTVDGDKLTISQAGSTMMACDPPEAMQQEMDFL
                     AALQSAATFAIDGDQLTIANEAGETVLTFKAAVQAALVGTNWVATMYNTGTEAVTNVL
                     EGTTITANFAEDGSLNGSAGCNNFMSSYTLDGQNVTIQPPATTRKLCPEPAGIMEQEA
                     AFVSMLPQATTYTISGSNLELRTAEGALIASFVTAQ"
     gene            complement(4130..4438)
                     /locus_tag="PLC06_07495"
                     /pseudo
     CDS             complement(4130..4438)
                     /locus_tag="PLC06_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019454731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
     gene            complement(4658..6346)
                     /locus_tag="PLC06_07500"
     CDS             complement(4658..6346)
                     /locus_tag="PLC06_07500"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRO90326.1"
                     /translation="MKDSQSPRAARRGPRMFTVSLLIFLLLALTTAVALPQEQAAEGP
                     YPTDAEVAATLKPVVLSESRSGDMTTLTVEIPVGADTFTTSNQPTTNWSNDPNMRIGF
                     NIAQGNGAQRAFLFFPMDSIPSNATVTAANLRVFQNAFSPPADVNMGALARFLSSSWD
                     ASTLTWANFNPSWGAEIGIGQLPATTGWIQASITSSVQEWVSGTRPNFGIMVQGDETP
                     QQRERVFTTINANNGLHPRIVVTYNVNVDTTPPTSSMTALPQWSTASFNVNWSGTDNP
                     GGTGIRHFDVQMRQNGGNWQNWQTAVTNTSATFTGQNGILYEFRARAVDRANNVEAFP
                     NNPQARTTVDTIPPTSSVNALPQYTFTRNFTVSWSGTDALSGIAHYDVQFQINGGPWQ
                     ALTSVTTQASHTIADALVGAQYGFRVRAVDRAGNVQPFPGGAQAQTTISVGNPEAHII
                     PFTSPIATQNSFVVQWFGEPVPGSNITSYDLQFSFNNGPWRDWLTNLPGGNGANNRTF
                     TAQDGDGVYAFRVRARDGVGRVGEFRGGLGATIAVDTVAPFITIQSYFPTIYGE"
     gene            6789..7643
                     /locus_tag="PLC06_07505"
     CDS             6789..7643
                     /locus_tag="PLC06_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007258381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRO90327.1"
                     /translation="MTKTIITAALTGVLTTRTQSPAIPYTPDEIAEEARRSVEAGASI
                     VHIHARQDDGRPAYDVDTYARIDAAVRARCPDVIINYSTGAIGVTREERVAHIAALRP
                     DMAALNMGSMNYAIYSEKNKMFYHDHVFANPFGDILFFLEAMNAAGTRPEMECFDTGH
                     IHNAAPLIDLGVLKPPYQFSLIMGVLGGIPGTTKNLVQQVDALPAGAHWQVIGIGARQ
                     WPLAAAAVSLGGNVRVGLEDNLYVAPGQLARSNGELVAKAAELVRLVGGEVATVEEAR
                     EMLELPLQ"
     gene            7670..7963
                     /locus_tag="PLC06_07510"
     CDS             7670..7963
                     /locus_tag="PLC06_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRO90328.1"
                     /translation="MIRSFRDRASEDIFNGVNSAVARRACPRQLWRIASRKLDQLDSA
                     ETLEELLFPPGNRLEALKGDRQGQHSIRINEQYRICFIWRDNAPQDIEITDYH"
     gene            7977..8279
                     /locus_tag="PLC06_07515"
     CDS             7977..8279
                     /locus_tag="PLC06_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRO90329.1"
                     /translation="MVRVPTHRSPTHPGEMLLEEFLNPMGITQRELADAIHVPYQRVN
                     EIINGKRGVTPATALRLARFFGMSAGFWMNLQLRWDLYQAQQEEAEVLATIEPHAV"
     gene            8282..8515
                     /locus_tag="PLC06_07520"
     CDS             8282..8515
                     /locus_tag="PLC06_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90330.1"
                     /translation="MDKPRKIALVYRKMRINEQGSDREYWLSQPPGARLAALEEIRCE
                     VHGWEPGEEPRVERVITIIKRGHSQSADKQAQE"
     gene            8520..8834
                     /locus_tag="PLC06_07525"
                     /pseudo
     CDS             8520..8834
                     /locus_tag="PLC06_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
     gene            8890..9225
                     /locus_tag="PLC06_07530"
     CDS             8890..9225
                     /locus_tag="PLC06_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90331.1"
                     /translation="MIEQYQHMTLVIENGKVIKVDNPQNSGVPFWESLKAGVLEPLPG
                     SLVNQTVAFALNQLSAQKWEVINLPPGNSQPVEAPRPFSLWPGLTPHPTPAPNTGSYE
                     ILLRKVIRK"
     gene            9265..>9754
                     /locus_tag="PLC06_07535"
     CDS             9265..>9754
                     /locus_tag="PLC06_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRO90332.1"
                     /translation="MAVAFQSSLSPRSDDYAANRAAMQVLIDELNERQARARAGGGER
                     GARKFREAGKLLPRERVEMLLDPATPFLELSPLAAEDMYNGESPAASQITGIGRVSGV
                     ECLIFANDATVKGGAVYPMTLQKTLRAQQIAFENRLPCISLVESAGANLLYQDEVFIL
                     GGR"
BASE COUNT         2159 a         2856 c         2771 g         1968 t
ORIGIN      
        1 gattggtaat ctcgtagtgc aagatcaact ctttcaggtt cggctgaact tcgacaattt
       61 caagatgggc caccggatcc ggcatggcat ccgtggtgaa gaagttgctg aagcggcgag
      121 tgccgttatc cagcgtctcc actgtacatg cggtccactg gggattgccg gtcagggtac
      181 aactttgctg atacatcgcc gactggaaga gttccaggcc gcgcgtcagg aacgggttgc
      241 cgtcgattac ctgtcccggt gaatacgtcc ccggagtaag gtgctcatag gggataccct
      301 taatgccgcc ggggccttct tcaggcagca cctggatgct gacctcgcgg tcgggcgagg
      361 attgcacgcg gaactccggc gaacccatcc agttcgacac cagcatggcg gcgatgaaca
      421 gcatcgccac agtcaggccc acccggcggt cggcgtagcg gcggctcttg cccacttcaa
      481 tgtagggcat gacgatgaac gccaccaaca gcagcgggat gaagccgacg accagcgtct
      541 tgtcggccag cttcagccag ccctgcgacc aggacagata ccacggcgca acgacatgga
      601 gcggcgtgac gaccgggtcg gcgtgggtct ccaatggcgc gtcccataac cataacgcgc
      661 ccgctaccag gaacaaggtc gcgagggcag accacatcag ttcgctggtc aggatgtcgg
      721 gaatatagta ggtgcgctca tccttcggca cgcgcttggc ggtatcctca cccacctgct
      781 cgcggcccgg cggcaacgaa atgccataga gcacgacctt atagtagtgg acgaagaaga
      841 agatgccggc cagcagcggc agcagcagca cgtggagcaa ataccagcgc agcaagccgt
      901 ccgcacccaa ggccgggcca ccctgcagga tcaacatgat gttctcattg acgaaggccg
      961 gggccggagc cgccgcgcca ccgctgatga acaccgtcaa cgcccaataa gccaactggt
     1021 cccacggcaa cagatagccg ctgaagctca ggaagatcgt gaagaccaac agcaacatgc
     1081 cggtggccca ggtgaactgt cgcggcttct tgtagctgcc ggtgacgaaa gttctgagca
     1141 tgtgcatggc gaccaccagc accatcacct cggcacccag acgatgcatg ttgcggaaca
     1201 gttgacccaa aggcacattg cccaggatgt tccacatgtt gccgtaggcc gacaacgggc
     1261 tgggcgtata aaacaccatc aggataaggc cggtaatggt ctcccacacc aacatgaagg
     1321 tcgataacca gcccaaccgg aaggtcggat agatgttggt gacgcccgta tgataaaagc
     1381 tgggccggat atggagccag aaaccgtcgg catgcggccg caagcggggg ttcggccggc
     1441 gcggttcatc gccgcgcaag gcagcccgaa cgtcgttgat gttcatgcct gcggtcaggc
     1501 gctcgacaac atcatccgcc ttcgtgacga tagcctgctt aatccccatt tcgcggactt
     1561 gttcgacgaa tgtagccata acaactcctc caaataatta agtatcagga aaatgggctt
     1621 tgattgtccc tcattcctga ttcgtaattc gtaattgatt aagggcagtt gccctggaaa
     1681 tcacacaaca acggttggga cgggccttgc ttgcgattgc cggtgtccac ccgaataaaa
     1741 gccgtcccgc tgggcagttg caacggctgg aagaaattat cggtcaatga agcctcggcc
     1801 agcacggtcg tctcgtcatc ggcaatggcc tggacgaaga aacgatcaag cgtgcgggga
     1861 gcgggcgatt ccacacgccg gccgtcgagc cgatatttcg acccgtggca cgggcactcg
     1921 aaccggctgt tggtcggcgt ccaggagtag atacagccca gatgggtgca gaccttatag
     1981 atggcggcga tgccctgaat ggggttcggc tcatcggggg cctgatacat taactcattg
     2041 ggttgcgtgc tatccaggtt cactaaccag aagcgcccct cggcgtagtt gacaggtggc
     2101 gtgttgatgt caggcacatc accggcagcc accgtgaatg ttccgccaaa ttcaccttca
     2161 cggaaacgag ggatcaaaaa ccacacaagc aatcccgcgc cttgcaatga cacaagcgcg
     2221 atcgaggccc cccatatgta atagagaaat tcgcgccggc tgacgccgcc ggaggctccg
     2281 gccccggatt ctgctgttgc cgtttgactg gccatatggc tactctcctg cctctctttc
     2341 tttcagatat aaatccattt gtcgtttcac tatctttaat cgaatgcggc cctctggcga
     2401 ccgcttgatt atcgctttgt acgctatatc acgaaagcga attatagcaa aaccacttgg
     2461 accaacaata acgagccatt cggctcattg atgggcgctg agggactcga acccccgact
     2521 tcttcggtgt aaacgaagcg ctctgaccaa ctgagctaag cgcccaaacg ccgccgcggc
     2581 cgataatcag gccgacgcag ggcgaaatta tagcgtttgg tgaggaaata cgccaattta
     2641 cagcggtcaa ttcggccgat aaatagatct cgcccgccta aatctggggc aagacgatac
     2701 tttgctggaa ctgatatttg cctttgcgat cggcatagga cgtctcgcac acctcatcgg
     2761 cctgaaagaa cagcacctgg gcgataccct cattggcata aatgcgcgcc ggcagcggcg
     2821 tggtgttgga gatttccaac gtcacatacc cttcccactc cggctcaaaa ggggtgacgt
     2881 tgacgatgag gccgcagcgg gcataggtgc tcttgcccag gcaaacggtg atgacgtcgc
     2941 gcgggatgcg gaaatactcc accgtttggg ccaagacaaa ggaattgggc ggaatgacgc
     3001 aggtatcgcc gtggtagtcg acgaacgatt gaggattgaa gttctttggg tcaacgatgg
     3061 ccgaatggac gttggtaaat atcttgaact cgttttttac ccgtatgtca tagccataag
     3121 acgacaggcc atacgagatt accccgtcgc gcacctgccc atcaatgaac ggctcgatca
     3181 tgttcttctc ttccaccatg cgacgaatcc agtggtcggg cttaactgac atgcaatact
     3241 cctcgtttgc ggccatctcc tggccaagaa tgagcaaatt gtggccgatt gccccgaaaa
     3301 acgcaaaaac gccgggccgc aaggaatgta ttcccctgcg acccggcgtc tgccgattga
     3361 atggttgtcg cggttattga gcggtgacaa atgaggcgat tagcgcgcct tcggccgtgc
     3421 gcagctccag attactgccg ctaatcgtgt atgtggtcgc ctggggcagc atgctgacga
     3481 aagccgcttc ctgctccatg atcccggccg gctcggggca cagcttgcgc gtcgtggccg
     3541 gcggctggat ggtgacgttc tgcccgtcca acgtatagct gctcatgaag ttgttgcacc
     3601 cggccgaacc gttcaggctg ccgtcctcgg caaagttggc ggtgatggtt gtgccttcca
     3661 gcacattggt caccgcttcc gtgccggtgt tgtacatcgt ggcgacccag ttcgtgccga
     3721 ccagcgcggc ctggaccgcg gccttgaagg tcaggaccgt ctcgccggcc tcattggcaa
     3781 tcgtcagttg gtcaccgtcg atcgcgaatg tggcggccga ctgcagcgca gccaggaaat
     3841 ccatctcctg ctgcatggct tccggcgggt cgcaagccat catcgtcgat cccgcctggc
     3901 tgatggtcaa cttgtcgccg tcaacggtat agccggcaaa gtagttattg caggtggcat
     3961 tgccggccac gcgcccgtcg gcggcgaact cggccgtcgc ctcgcggtca gccaacgcct
     4021 cgacgacctc accggcggcg tttacgtagg aagtcaacac ccaacgcact ccagtcaatt
     4081 catttccctc caccggcttc gtgccgtctt ctccggccgc aaccggcgtc ttgttcacta
     4141 tctcaaccag cgtataccgc agggacgagc cgtcggcggg tggattctct acctgctcaa
     4201 ctcgcaccag cagttcatat tcataacccg gctcaaaggt gaagccctca atattgctat
     4261 agaaaaactg atattcgccg gtcggatctt cgcgcacctg aaggcactgc tggggaacga
     4321 cgccgacaca atcaaccagt tgtgggccaa cgaacaaggt cttcgtctcg ccctgcaagg
     4381 cgctctcagg ctcgctgccg gtcgccgtgc aggccacaac agcaagggca agcaccatca
     4441 gggcagccac aacaaaaccg tacctactct tcatcatctt ttattcctct atcttgatac
     4501 tacaaacccg tagtgatgtt atccggaaca gtttccggca tgcgtcttaa ttcgcgcaca
     4561 ctattaagac gtcagcccaa aagaaaagtt ccatccatca caaaacgaag agccgggcgt
     4621 ttgaggcgtc cggctcttca tcagtcagct tgttgggtta ctcgccgtaa atcgtgggga
     4681 agtagctctg gatcgtgatg aaaggcgcca cggtatcgac cgcgatagtg gcgcccaggc
     4741 cgccacggaa ctcacccaca cggccgacgc cgtcgcgagc acgaacgcgg aaggcgtaga
     4801 cgccatcgcc gtcctgagca gtgaaagtcc gattattggc cccattgccg cccggcaggt
     4861 tggttaacca atcccgccag gggccattgt tgaagctgaa ttgcagatca taggaagtga
     4921 tgttcgagcc gggcaccggc tcaccgaacc actgcacgac gaagctgttt tgcgtggcaa
     4981 tcggtgaagt aaaggggatg atatgcgctt ccggattgcc cacggagatg gtcgtctgcg
     5041 cctgagcgcc gcccgggaac ggctggacat tgcctgcccg gtctaccgcc cgcacccgga
     5101 agccatattg cgcgcccacc aaagcatcgg ctatcgtgtg agacgcctgg gtcgtaaccg
     5161 aggtcagcgc ctgccacggg ccgccgttga tttggaactg cacgtcataa tgggcgatgc
     5221 cactcagcgc gtctgtacca ctccagctaa ccgtgaagtt gcgggtgaag gtatattgcg
     5281 gcagagcgtt caccgacgac gtcggcggga tggtgtcgac cgtcgttcgc gcctgggggt
     5341 tattggggaa tgcttcgacg ttattcgccc ggtcgactgc ccgcgcgcgg aactcataaa
     5401 ggataccgtt ctggccggta aatgtggccg acgtgttcgt gacggccgtc tgccagtttt
     5461 gccagttgcc gccgttctgg cgcatctgga cgtcgaaatg acggatgccc gttccgcccg
     5521 gattgtcggt cccgctccag ttcacgttga acgacgcggt cgaccattgc ggcagcgccg
     5581 tcatgctcga agtaggcggc gtggtatcaa cgttcacatt ataggtcacg acgatgcggg
     5641 gatggaggcc gttattggcg ttgatggtgg tgaaaacgcg ctcccgttgc tgcggtgtct
     5701 cgtcaccctg aaccatgatg ccaaagttgg ggcgcgtgcc cgatacccac tcctgcactg
     5761 agctggtgat ggaagcctgg atccagcccg ttgtcgcggg cagttgacca atgccgatct
     5821 ccgcgcccca gctggggttg aagttcgccc acgtgagcgt tgacgcatcc caggaggaac
     5881 tcaggaagcg cgccagcgct cccatgttga cgtccgcggg aggcgagaac gcgttctgga
     5941 aaacgcgcag attggcggcg gtcacggtcg cgttggaggg gatgctgtcc atcggaaaga
     6001 acaaaaatgc ccgctgcgct ccattgcctt gagcgatgtt gaatccgatg cgcatgttgg
     6061 gatcattgga ccagttggta gtcggctgat tggacgtcgt gaacgtatcc gcaccgacgg
     6121 gaatctccac tgtgagcgtc gtcatgtcgc cgctgcgcga ttcactaaga actacaggct
     6181 tgagggtcgc cgctacttcc gcatccgtgg ggtatggccc ctcagcagct tgctcctgag
     6241 gcagcgcgac ggccgtcgtc aacgccagca atagaaagat gaggagggaa acagtgaaca
     6301 tccgggggcc acgacgggca gcccggggag actgggaatc cttcatattt gtaagctcct
     6361 tactatggtg atgtttgtcc cggtaaaagc gacgcgggct cacacaggca caacttcctc
     6421 ttcccagggt cgcggacaga ctccccttca tggaattgag cctcgggcag gtataattgc
     6481 ccgattccca taaaacgtcg cttttgccat tatagtcgtc aagcgtctca aatatcaata
     6541 gaaatccaga ttcttgtgtc tgtcaattag ccaaatgtac tatgatggca tcctcgattc
     6601 cttctcctgt ggtgcttatc gcaggatttc aaacgtatct tgcctcacaa tcaggtcgat
     6661 ttccagcgag gacctgtcca ccgccggcgc tggagcatca caataatgac gtcgtgcaga
     6721 gagagcaaac aggattaaat gaccaactcg cgttagaata accctatgcc caaaattgga
     6781 gccattccat gaccaaaacc atcatcaccg ccgccctcac cggcgtgctg accacccgca
     6841 cacagtcgcc ggccatccct tacactcccg acgagatcgc cgaagaagcg cggcgcagcg
     6901 tggaggcggg cgcatctatc gtccacatcc acgcccggca ggacgacggc cgcccggcct
     6961 acgacgtgga cacctatgcc cgcatcgacg cggccgtccg cgcccgctgc cccgacgtca
     7021 tcatcaacta ctccaccggc gcaatcggcg tcacccgcga ggagcgtgtg gcccacattg
     7081 ccgccctgcg ccccgatatg gccgctctta acatgggcag catgaactac gccatctact
     7141 ccgaaaagaa caaaatgttc tatcatgacc acgtcttcgc caacccgttc ggcgacatcc
     7201 tgttcttcct ggaagcgatg aacgctgccg gcacgcggcc ggagatggag tgtttcgaca
     7261 ctgggcatat ccacaacgcc gcgccgctga tcgacctggg cgtgttgaaa ccgccgtatc
     7321 agttcagcct catcatgggc gtgctgggcg gcattcccgg cacgaccaag aatctggtgc
     7381 aacaggtgga tgcgctgcct gccggcgcgc attggcaggt catcggcatc ggcgcgcgcc
     7441 aatggccgct ggcggccgca gccgtttcgc tgggcggcaa cgtccgcgtg gggctggagg
     7501 acaatcttta cgtagcgcca gggcagctgg cgcggtcgaa cggggaactt gtggccaagg
     7561 cggccgaact ggtgcgtctg gtgggcgggg aggtggcgac ggtagaagag gcgcgggaga
     7621 tgctggagtt accattgcag tagcatgggt cccatgttat catgttatca tgatccgcag
     7681 cttcagggat cgagcaagcg aagacatttt caatggggtg aattctgctg ttgctcgccg
     7741 cgcctgtcca agacaattgt ggcgtattgc ctctcgaaag ctcgatcaac tcgattccgc
     7801 ggaaacgctg gaggaacttc tatttccgcc cggcaaccgg ttggaagcat tgaagggtga
     7861 tcggcaaggg caacatagta ttcgcatcaa tgagcaatat agaatttgtt tcatatggcg
     7921 agataacgcc ccacaagaca tagagataac cgattatcat taaaggaaaa tctattatgg
     7981 ttcgtgtgcc aactcatcgc tctcccactc atcccggtga aatgctactg gaagagtttc
     8041 taaacccaat gggcatcacc caacgggaat tggccgacgc tattcacgtt ccctaccagc
     8101 gggtaaacga gatcatcaac ggcaagcgtg gagtaacccc ggcgaccgca ttgcggctgg
     8161 cgcgcttctt cggaatgagc gccgggttct ggatgaatct gcaattgcgc tgggaccttt
     8221 atcaggcaca acaggaagag gctgaagtcc tagcaacgat tgagccacat gctgtctaac
     8281 catggacaaa ccaagaaaaa tcgccctcgt ctatcgcaag atgcgcatta acgagcaggg
     8341 ttccgatcga gaatactggc tctcccaacc tccgggtgcc cgcctggccg cactggaaga
     8401 aatccgttgc gaggttcatg gctgggaacc tggcgaggaa ccgagagttg agagagtgat
     8461 tacaatcatc aaacgcggtc attcacaatc tgccgacaag caagcgcagg aatagttgta
     8521 tgaacgtacc cgttttagat cagcatatcg aagtaacggc atatgtagcc ggcggcaagc
     8581 tacacattgc cggatgatgc atcaccgtgc aaaacatcgc catctggcat gaatacatgg
     8641 gcttgagcgt tgatgagatt gccaccgatt atagcctctc cttggacgac atctatgccg
     8701 ctatggcgta ttattttgac catcaacaag cgatagatag gcaaatcgct gaggatgagg
     8761 cgttcgtgga tgcgcttcaa gcgcaaacgc cttctccgtt tcgccaaatg ttaacgcaag
     8821 acgagcttaa gtaacatccg ccgttaagct atatgcttga tcctgggaat atcaataagg
     8881 agggtacaaa tgatcgagca atatcaacat atgacgctgg tgatagagaa cggtaaagtg
     8941 atcaaggtcg acaaccccca aaactccggt gtcccttttt gggaaagtct taaggccgga
     9001 gttcttgagc cgttgccagg ctccctggtc aatcaaaccg ttgccttcgc tctcaatcaa
     9061 ctgagcgcgc agaaatggga agtcatcaat ctgccgcctg gcaacagtca gccggtcgag
     9121 gctcctcgcc cattttcctt gtggccgggc ttaaccccgc atccaactcc ggctcccaat
     9181 acaggcagtt acgaaatcct gcttcgcaag gtcattcgta aataaaaccg ataggttgag
     9241 ccaaacacaa acagggccag tgcaatggcg gtcgccttcc aatcctccct ttctccccgc
     9301 tccgacgact acgctgccaa ccgcgctgcc atgcaagtgc tgatcgatga gttgaacgag
     9361 cgccaggcgc gggcgcgcgc cggcggcggc gaacgtggcg cgcgcaagtt ccgcgaggcg
     9421 ggcaaactac tgccccgcga gcgggtggag atgctgctcg acccggccac cccctttctg
     9481 gaactttctc ccctggcggc cgaagatatg tacaacggcg agtccccggc cgcgtcacag
     9541 atcaccggca tcggccgtgt cagcggtgtc gagtgcctca tctttgccaa cgacgccacg
     9601 gtcaagggcg gcgcggtcta tccgatgacc ctgcagaaga ccctgcgcgc ccagcagatc
     9661 gccttcgaga accggctgcc ctgcatctca ctggtcgaat cggccggagc caacctgctc
     9721 tatcaggatg aggtgttcat cctcggcggc cgca
//
LOCUS       DAOMQG010000103         9602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3062_length_9602_cov_9.153975, whole genome shotgun sequence.
ACCESSION   DAOMQG010000103 DAOMQG010000000
VERSION     DAOMQG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_9602_cov_9.153975"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLC06_07540"
     CDS             complement(<1..560)
                     /locus_tag="PLC06_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90333.1"
                     /translation="MRKRTKSIILLVAVGQMLLILGLLALPAVVQAVPGRYRVWLQEN
                     YPAMGKISESVIAQVAPVATALPAAQQTSGTQIDISSLIAEQPVATLAGDAVEAIELP
                     QAATPAAPAVDATDAPAEPVASPTPAPTTTPAPTPTPMPLPSRVVLEDMGVVKQSFNN
                     CGPANLTQVLNWYGSDITQEDVASYL"
     gene            complement(598..1875)
                     /locus_tag="PLC06_07545"
     CDS             complement(598..1875)
                     /locus_tag="PLC06_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRO90334.1"
                     /translation="MPSILRKLPTRYAMRLPEPLQALALPVDSVPILPTVAAPDAAAG
                     LIARDMPPPAIIMATATADPTSQAVAPEADSTPVAAPVVPSPTTTPPPTATPWPVPTA
                     ARMTGFTHVYQEWNNCGPATMVMALTHFGRIHSQRDTAQILRPNPEDRNVTPQEMAQF
                     VNAQDGLRGMARVNGDLNLLKRFLANDIPVIIEIGLDPPGEYRWLGWYGHYLLPVAYD
                     DATGQIWVYDSWLGTSEIPQTNADPNGRVLTYDELEAYWAQFNRSFIVLYTAEQESLV
                     RDLLGEDVDDGIMWQRSLERAQADARRDAANAFYWFNLGTSYNALGQFEDAAVAFDQA
                     RAIGLPWRMLWYQFGPYEAYYRTGRYEDIVLLADTTLKDRPYFEESFYYLGLAQAALG
                     DDTSARDNLEKAIGFNPNFTPASDALAGLAAGG"
     gene            complement(2111..5275)
                     /locus_tag="PLC06_07550"
     CDS             complement(2111..5275)
                     /locus_tag="PLC06_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO90335.1"
                     /translation="MDVPHACVPTLIDHKNVSTTSSPSSPTRWRAITIVMLLLAALSL
                     LTLGALSARDTFLMRGIPAGLPEPIQNGGARLGINVELERYDGDDIEETLSAIKGLGI
                     EYVKQPFYYREDYQWETADRMVSAIRDQGLSLVPLLDGNSANGFMPPEDVILYADWAG
                     EFAQRYGDSISHYIIWDEPNLTSHWGGQPVNPNHYAALLTAAAERVRANDPDAMIVAA
                     PLAPTTETGPDNLSETLFLQALYEAGAADSFDIVAAKPYGFDTGPDDRAVEPALLNFS
                     RPIMLRELMVTQGDGHKAIWAGNWGWNSLPEGWTGRPSIWGQTSEAEQAAHTTAAFDR
                     ARREWPWMGLMFLENWEPNVPVDNPRWGFSIANRQTAAALTNYLAAQLAEVAMPGFHL
                     ARPDDPAQEFSGDWEFSPEFGADIGQSGDAARFTFWGTDVGLRVRRADFRARLYATVD
                     GQPANALPRDENGAMLILTSPDPTQDFISTEWVARDLPPGPHTLEITAARGWDQWALN
                     GFSVGYTPTEVSSPWIRMALWVTALVSFGLTWYCARRAEWGATGRATGAAFSRLSDRA
                     QLLLTAGAAALVALTGWLTWGSDALGIYRRLGDAGQLVATAAAAAVFYVTPSFVLFAL
                     ALGVLFILLVLRPAWGVVLIALTMPFYVPPLPKPILGYSFSPVEIFTLVATGAWLTRF
                     ALDKGMAARRGTAHFSWPKLSRPDWAVVAFLIIATLSLFVAEFQGVALSEWRVVILEP
                     VLFYMLLRGTKLHDKELWIVLDAFVVSGLIIALYGLWQYATGQDLITAEGGLMRLRSI
                     YGSPNNVALYLDRLLPLLVAILLLGTRATHGSRRLLYGLALAPVALALLLTFSKGALF
                     LGIPASLIVVFWVWQRRAGRRTWPWLVVGLMAGTGALLVASRVPALAARLDFFGATGV
                     FRVNLWRSAVNMFTEHPWLGVGLDNFLYLYRGRYILDAAWQEPNLSHPHNIILDFATR
                     LGLLGLLAGGWLIWEAFSALRRGIAQANAQWLPVAVGFAGSLAAMLAHGLVDHSFFLI
                     DLSFVFFLILGVAVWIQNKPNAGD"
     gene            complement(5242..6771)
                     /locus_tag="PLC06_07555"
     CDS             complement(5242..6771)
                     /locus_tag="PLC06_07555"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HRO90336.1"
                     /translation="MRVDGETRQLTTTAATVGEALKQTGIDVNSADEVDPPLSSALPK
                     DGGVSPLTITIVRVTETLEIIPESVPYGRRIVRSAEMSPTDAPRLLQTGTPGLQEVSV
                     RIVFRDGLEAERWPTSVRVIEPAVDEIVMIGVGQDREAMAITGKLAYLSNGRAILFDG
                     TTDTPSQLEIAGTLDGRVFELSPDGRYLLYTLDREDSGEETFGNELQVIETQPGAVGR
                     ALQIENVLWAGWDPSAVELPRIAYTTARSVPLPPGWEALNDLWLLSLPVEGQQTAPVR
                     IVESAAATFGWWGGIYAWAPGGDRIAYAFADEIGVLAVPSTDPAGDEALAPPSRTVLH
                     SFTAYETGADWAWLPNLGWSADGRLLSYTAYSAENERFDLWLTDTTTTDTIQFAENVG
                     MWAAAVWSPAVALPQASIAYLQAVEPTAGEESSYALWLAESDGSNKSRVFPPEGETGA
                     FDRTRHALAWGPDPNLIAFIFDEELHILDRVSNDLFRAGADDTVSSHPTWTSLTPASQ
                     P"
     gene            complement(6939..7352)
                     /locus_tag="PLC06_07560"
     CDS             complement(6939..7352)
                     /locus_tag="PLC06_07560"
                     /inference="COORDINATES: protein motif:HMM:NF021536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HRO90337.1"
                     /translation="MSNPSTAIDLDAFYQSMMQAGNLRTPQHASRLTFAVLHTLGFNL
                     SGSVKRDLAKSLPKDLANSLKRGWRLINIRHKNLSLHDFAKDVARNSGNTDAAVAETR
                     TRLVFHQIKKLLDGNLIHEVGRDLSPEVRDAWLAA"
     gene            7586..8224
                     /locus_tag="PLC06_07565"
     CDS             7586..8224
                     /locus_tag="PLC06_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRO90338.1"
                     /translation="MSERIVIEAEPREIIGKQTNKLRRDGWIPGVIYGRKTHTSVQME
                     QKALRRALRVVGTAHLADLSLNGTTHTVLVREIQQHATRGDIVHVDFLEVDMKTKLRS
                     SAEIVGTGVSAPEAAGLGDVTILTHSVEIECLPDDLVSEIEFNMALIATPDDVVYVKD
                     LVAPKGVEILDDPETVIARFEYVATGDEDLGDGASAEDVEIISRGKKEDGDF"
     gene            8237..>9602
                     /locus_tag="PLC06_07570"
     CDS             8237..>9602
                     /locus_tag="PLC06_07570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90339.1"
                     /translation="MTRTQRIALVVILLSAVTLRLTGLDWDGFQHHHPDERYITWVAT
                     TIEFPSIGQITEAAQWRPETSTFNPFHWPPGAGSEGIVVLQGEPRDFAYGHVPLYLGV
                     AATRLVEWVAPPLLRLLPADWSLTADVLNGAGRIEFHHLAAVGRFLTALFDAGTVLFI
                     FLLGRRLYGVAAGLLAAALLSVTVLHIQLAHFFTSDPYLTFFVVAAVYFMVAAHGDWA
                     AGPAGEGRGKWYFILAAIMVGLAIGSKFTAVLLSLPLLWTAAAIGPSSRRRYWALLGI
                     VVAALAFALTNPFAVLDWTCTPPSTGGGESGLSATLSRSCYIQNILTQNAMVSGRADL
                     GFTRQYAGTIPYVYFGEMLVRWGLGPLLGLVALAGLAVSAWDVARSLRGVAGINEALQ
                     RLLRQPVVIVLLWVLPYLLLTGGFYVKFLRYMQPIAPFLILFGAALLWRVRRPSVRYA
                     LAGIV"
BASE COUNT         1908 a         2771 c         3026 g         1897 t
ORIGIN      
        1 ttcaggtacg aggcaacgtc ttcctgggta atatcggatc cgtaccagtt caatacctgg
       61 gtcagattgg ccgggccgca gttgttgaag gactgcttga ccacccccat atcttcaaga
      121 acgacccggc tgggtagcgg catgggagtg ggtgtcggag ccggcgtggt tgttggtgca
      181 ggagtgggcg aggcgaccgg ttcggccggc gcgtcagttg catcgacagc cggagcagca
      241 ggtgtggcgg cttgtggcag ttcgatggct tccactgcgt caccggccaa ggtggcgacc
      301 ggttgttcag cgatcagcga tgagatgtca atctgggttc cagaggtctg ttgggcggcc
      361 ggcagcgcgg tagctacggg agccacctgg gcgatcacgc tctcagagat tttacccatt
      421 gccgggtagt tctcctgcag ccacacccgg tagcggccgg gaacggcttg taccacggcc
      481 ggtaaggcca acaggccgag gattaacaac atctgtccta cagcgacgag caggataatc
      541 gatttggtac gtttacgcat gataaatagt gttctccgtc tcccggcgcg tcactcgtca
      601 gcccccggcc gcaagaccgg ccagagcatc cgaggctgga gtgaaattgg gattgaagcc
      661 aattgctttc tccagattat cgcgtgcgga tgtgtcatca cccagggccg cttgcgccag
      721 acccaggtag tagaatgatt cttcaaaata ggggcgatcc ttgagcgtgg tatcggccag
      781 caggacgata tcctcatagc gcccggtccg gtaataggcc tcgtaggggc cgaactgata
      841 ccacaacatt cgccagggca ggccgattgc ccgtgcctga tcaaaggcga cggccgcgtc
      901 ttcaaattgt cccagcgcgt tataactggt gcccagattg aaccaataga aagcgttggc
      961 ggcgtcacgc cgggcgtcgg cctgggcgcg ctccaggctc cgctgccaca tgatcccgtc
     1021 gtccacgtcc tcgcccaaca ggtcacgaac caggctctcc tgctcggccg tatacagcac
     1081 gatgaaagag cggttgaact gtgcccaata ggcttccagt tcgtcataag tcaggacgcg
     1141 gccgttggga tcggcattgg tctgcgggat ttcactggtg cccagccatg agtcgtagac
     1201 ccaaatctgg ccggtagcgt catcgtaggc aaccggcagc aaataatggc cataccaacc
     1261 cagccaacga tattcgccgg gcggatcaag cccgatctca ataatgacag ggatgtcgtt
     1321 ggccaggaat cgcttaagga ggttcagatc gccattaacc ctggccattc cccgcagacc
     1381 atcctgcgcg ttgacgaatt gggccatctc ttgcggcgta acgttgcgat cttccggatt
     1441 cggtcggagt atctgcgccg tgtcgcgttg gctatggatc cgcccaaaat gggtcagagc
     1501 catgaccatc gtcgccggcc cacagttatt ccattcctgg tagacatggg tgaagccggt
     1561 cattcgcgcg gcggtaggga cgggccaagg agttgccgtg ggtggcggcg tggtcgtcgg
     1621 gctgggcaca accggcgcag cgaccggggt tgaatctgcc tcaggggcaa cggcttggga
     1681 tgttgggtcg gccgtcgccg tggccataat aatggcgggc gggggcatgt cgcgggcgat
     1741 gaggccagcg gcggcatcgg gcgcggcaac agtcggcagg atggggaccg aatcgaccgg
     1801 caaggccaac gcctgcaaag gctccggcaa acgcatggcg taacgcgtcg ggagtttgcg
     1861 taatatggac ggcacaatac cgatcgctat gattcccgcc acaacaagga cgagaagtaa
     1921 gagtagccgg tttcttcgca tggattcatc gatcgcgggt cattcaaccc gaccggagag
     1981 gattgtagcg tattgtcaaa ggcggtggaa gatgagcgcg gattggttca gcatattcta
     2041 acgccgccgc aagggccttt gtgcaatgga gaataacccg gtatatcgcg ttttctcggt
     2101 taatcggcag ctaatcaccg gcgtttggtt tattttgtat ccagaccgcc acgcccagga
     2161 tcaggaagaa gacaaagctg aggtcgatga ggaagaagct gtggtctacc aggccatgag
     2221 ccagcatagc cgccagcgaa ccggcaaacc caaccgctac cggcagccat tgtgcgttgg
     2281 cttgcgcaat tccacgacgt aacgcgctga acgcttccca tatgagccac cccccggcga
     2341 gcagccccag caggccgagc cgggtggcga agtcgaggat gatattatgg ggatggctca
     2401 agttcggttc ttgccaggcg gcatcgagga tgtagcggcc gcggtagaga tagaggaagt
     2461 tgtcaagccc gacccccagc cagggatgtt cggtgaacat attgaccgcg gagcgccaga
     2521 gattgacgcg aaacacaccg gttgcgccga aaaaatcgag ccgcgcggct aacgcgggaa
     2581 ctcgactcgc cacaagaagc gcgcctgttc cggccattaa ccccacgacc agccagggcc
     2641 aggtgcgccg gccggctcgc ctctgccata cccagaacac cacgatgagc gatgccggga
     2701 tgcccaggaa cagcgccccc ttgctgaatg tgagcagcaa ggcaagagcc accggggcaa
     2761 gcgcgagacc ataaagtagc cggcgagaac cgtgagtggc gcgcgtgccg aggagcagaa
     2821 tggcgaccaa caaggggagc agccgatcca gatagagggc cacgttgttg ggcgagccat
     2881 agatcgagcg aagacgcatc agtccacctt cggccgtgat caggtcttgg ccggtggcat
     2941 attgccacaa gccgtagagg gcaataatca gcccgctcac cacaaaggca tcgagcacga
     3001 tccatagctc cttgtcatgc agtttggtcc cgcgcagcaa catgtaaaac aggacgggtt
     3061 cgagaatgac gacgcgccat tcgctgagag ccaccccctg aaattcggcg acaaagaggg
     3121 agagagtggc aataatcagg aacgccacaa ctgcccagtc cggtcgcgac agtttgggcc
     3181 atgagaaatg ggccgtccct cgtcgcgcag ccatcccctt gtcaagggcg aacctcgtta
     3241 gccatgcgcc tgtggcgacg agcgtgaaga tctctaccgg agagaagctg tagcccagta
     3301 tgggtttagg cagaggcggc acgtaaaacg gcattgtcag ggcgatcagg acgacacccc
     3361 aggccgggcg taacaccaac agaatgaaca gcacacccag agccagcgca aacaatacga
     3421 aggacggagt gacatagaac acggccgcag ccgcggccgt ggcgaccagc tgtccggcat
     3481 ctcccagccg ccggtatata cccagagcgt ccgaacccca ggttagccag ccggtgagcg
     3541 ccacaagcgc cgccgcgccg gccgtcagca gcaactgcgc cctgtcagac aggcgagaaa
     3601 aagcagctcc ggtggcgcgc cctgtcgcgc cccattcggc gcgccgggca caataccaag
     3661 tgaggccgaa cgaaacgagg gccgtcaccc aaagtgccat ccgaatccat ggactggata
     3721 cctcagtcgg agtatagccc acgctgaacc cgttcaacgc ccactgatcc cagccgcgcg
     3781 cggcggtgat ctccaatgtg tgcgggccgg gcggcagatc gcgagccacc cattccgtgc
     3841 tgataaagtc ctgggtcggg tcgggcgaag tgaggatgag cattgctccg ttctcatctc
     3901 gcggcaaggc attggcgggc tgcccgtcaa ccgtggcgta cagtcgcgcg cgaaagtcgg
     3961 cccgtcgcac gcgcaacccg acatcggtgc cccaaaacgt gaagcgagcc gcgtcgcccg
     4021 attggccgat gtcggcgccg aattcaggcg agaactccca atcgccgctg aactcctggg
     4081 ccggatcgtc cgggcgggcc agatgaaagc ccggcatcgc cacctcggcc agttgcgcgg
     4141 ccaaatagtt ggtcagagcg gcggccgtct ggcgattggc gatgctgaac ccccatcgcg
     4201 gattatccac cggcacgttc ggctcccaat tttcaaggaa catcaggccc atccatggcc
     4261 attcgcgacg agcgcggtcg aacgcggccg tggtgtgggc tgcctgttcc gcttcgctgg
     4321 tttgccccca tatcgatggc cgccccgtcc agccttcggg caggctgttc cagccccagt
     4381 tgccggccca gattgctttg tggccgtctc cttgggtcac catcagttca cgcaacatga
     4441 tcgggcggct gaaattgagg agcgccggct cgacggccct gtcgtcgggg ccggtgtcga
     4501 agccgtaggg tttggcggcg acgatgtcga aagagtcggc cgcgcccgct tcgtagagcg
     4561 cctgcaggaa taatgtttcc gacaagttat ccgggccggt ctcggtggtg ggcgcgagcg
     4621 gcgcggcgac gatcatggca tcggggtcat tggcccgaac tctctcggcg gcggccgtta
     4681 gcagggcggc ataatgattg gggttgaccg gttggccacc ccaatggctg gtcaggtttg
     4741 gctcgtccca gatgatgtaa tgggagatag aatccccata gcgttgggcg aactcaccgg
     4801 cccaatccgc gtaaagaatg acatcttcgg gcggcatgaa tccgttggca gaattgccgt
     4861 cgagcagggg gacgagcgac aggccctgat cgcgaatcgc gctgaccatc cgatcggccg
     4921 tttcccactg ataatcttcg cgatagtaaa acggctgttt gacgtattcg attcccaacc
     4981 ctttgatggc cgataaggtt tcctctatat catcgccatc atagcgctcc agttcgacgt
     5041 tgatccccag gcgcgcgccg ccgttctgga ttggctccgg cagtccggcg ggaatgccgc
     5101 gcatcaggaa tgtatcgcgc gcggacaggg cgcccagcgt caacaacgat agcgcggcaa
     5161 ggagcagcat cacaatggtg atggctcgcc aacgggtagg ggaggagggg gaactcgtgg
     5221 tggacacgtt tttgtgatcg atcagggttg ggacgcaggc gtgagggacg tccacgtcgg
     5281 atggctgctc accgtgtcat cagctccggc gcggaacaag tcgttggaca cccgatcgag
     5341 tatgtgcagc tcttcgtcga agatgaacgc gatcaggttt gggtccggcc cccatgccag
     5401 tgcgtgacgg gtgcgatcga aggcccccgt ctctccttca ggtggaaaaa cccgcgactt
     5461 attgctgccg tcgctctcgg ccagccataa ggcgtagctg ctctcttcgc ccgcggttgg
     5521 ttcaaccgcc tgcagatagg caatgcttgc ctgtggcaaa gcaaccgcgg gcgaccaaac
     5581 ggcggcggcc cacatgccga cattctccgc gaactgtatt gtgtcggtgg ttgtcgtgtc
     5641 ggtcagccat aaatcgaagc gctcgttttc ggccgaataa gcggtgtaac tcagtaggcg
     5701 accatcggcc gaccacccca aattcggcag ccaggcccaa tccgcgcccg tttcataggc
     5761 tgtgaaactg tgcaggacgg tgcgagacgg tggagctaac gcttcgtctc cggccggatc
     5821 ggttgaggga acagcgagga cgccgatctc atcggcgaaa gcgtaggcga tccggtcgcc
     5881 gccgggtgcc caggcgtaga tgccgcccca ccagccgaag gtcgccgcag cggattcaac
     5941 aatcctgacg ggcgcggttt gttggccctc gaccgggagc gataacagcc acagatcatt
     6001 gagcgcttcc cagcccggtg ggagagggac ggatcgggcc gtcgtgtaag cgatgcgcgg
     6061 cagttccacg gccgacgggt cccatcccgc ccacaggacg ttctcgattt gcagcgctcg
     6121 ccctacagcg cccggttgtg tctcgatgac ctggagttcg ttgccgaatg tttcctcgcc
     6181 gctatcctcg cggtcgagcg tgtaaagaag ataccgccca tctggcgata attcgaagac
     6241 gcggccgtca agagtcccgg cgatctccaa ttggctgggg gtgtcggtgg tgccgtcgaa
     6301 gagaatagct cggccgttgc tgagataggc cagcttgccg gtgatagcca tcgcctcgcg
     6361 atcttggccg acgccaatca tcactatctc atccacggcc ggctctatca cccgaacgga
     6421 tgtgggccag cgctcggcct ccaatccgtc gcggaacacg atacgcacgc tgacttcctg
     6481 caggccgggc gttcctgtct gcaagagtcg gggcgcgtca gtaggcgaca tctcggcact
     6541 gcgcacgatg cggcggccgt aaggaacgct ttcggggatg atttcgagtg tttccgtcac
     6601 gcgaacgatg gtgatggtga gtggcgagac cccgccgtcc ttggggagcg cactcgatag
     6661 cggcgggtca acctcatcgg ccgagttcac atcgatgccc gtctgtttaa gcgcttctcc
     6721 cacggtggca gctgttgtag ttagctgccg cgtctcgcca tcgacgcgca cttccaggga
     6781 actgatcgcg ggtggggcgg ccgacaacga ttgaacaggt gaggcggatg ttttgcacga
     6841 ggacaagaaa agcaggcagg ccgcgaaaac gcacagcaga cctgcccgcc catcaagtgg
     6901 attgaggaac gcagaacgcc gcaaaatagt tgcactacct aggccgcgag ccacgcatcg
     6961 cggacttcag gactcagatc gcggcccact tcgtggatca agttgccgtc gagcagtttt
     7021 ttgatctggt gaaaaacaag ccgcgttctg gtctcggcga cggcggcgtc tgtattgccg
     7081 ctgttacggg ccacatcctt ggcgaagtcg tggagcgaca ggttcttgtg acgaatgttg
     7141 attaatcgcc agccgcgctt cagagagttg gccagatcct tgggcaggga tttggccagg
     7201 tcgcgtttca cgctgccgct aagattgaat ccgagtgtgt ggagcacggc gaatgtgagg
     7261 cgagaggcat gctggggagt ccgcagattc ccggcttgca tcatggattg gtaaaaggca
     7321 tcaagatcga ttgcggttga tgggttgctc ataaaaatca ctgcctggat tgacttgaaa
     7381 tattttgtcg ctatacttct atattcgatc tggtttcaca gattatagcc gacaagtcat
     7441 ttaataagga atatatccgc agcggcagtg gcgatcaaat gagttggcca cattcgttga
     7501 gcacgcgtgt tgggcaccga tagtgaacat cgcaggtgcc tacgccgaaa atttccaggc
     7561 cgctgcagac aagggtagac agagcatgag tgagagaatc gttattgaag cagaaccgcg
     7621 cgagattatc ggcaagcaga ccaataagct gcggcgcgat ggctggattc ccggcgtcat
     7681 ctacgggcgc aagacgcaca caagcgtcca gatggagcaa aaagccctgc gtcgcgctct
     7741 gcgcgtggtc gggactgctc atctggccga cctgtcgttg aatggcacga cccacacggt
     7801 gctggttcgc gagatccagc agcacgctac gcgtggcgat atcgttcatg tcgatttcct
     7861 ggaagtcgat atgaagacga aattgcgctc ttcggccgaa atagtcggca ccggcgtatc
     7921 tgcgcccgag gccgcgggcc tgggtgatgt caccatcctg acccactccg tcgaaatcga
     7981 gtgtttgccc gacgatctcg tatccgagat cgaattcaat atggcgctca tcgctacgcc
     8041 cgatgatgtg gtgtacgtca aggacctggt cgcgccgaag ggtgttgaga tcctcgacga
     8101 tcctgaaacg gtaatcgccc gctttgagta tgtcgccacg ggagacgaag acctgggcga
     8161 tggcgcttcg gccgaggatg tcgaaatcat ctccaggggc aagaaggaag acggggactt
     8221 ttagtctgct tctgccgtga cccgaacgca gcgaatcgca cttgtcgtta tcctgctgtc
     8281 ggccgtcacg ttgcgtttga ccggcctgga ttgggatggc tttcaacatc accatccgga
     8341 tgaacgatat atcacgtggg tagccacgac gatcgagttt ccctcgattg gacaaataac
     8401 cgaagccgcg cagtggcggc cggaaacatc gacattcaat cccttccact ggccccctgg
     8461 ggccggcagt gaagggattg ttgttttgca gggggagcca cgcgatttcg cctatggtca
     8521 tgtgccgctg tatctgggtg tggccgcgac caggttggtg gaatgggtgg ccccgcctct
     8581 gttgcgccta ttgcctgcgg actggagcct gactgccgat gtgctgaacg gagcagggcg
     8641 gatcgaattc catcatctgg cggccgttgg tcgattcctg actgcgttgt tcgacgccgg
     8701 cactgtcttg ttcatctttc tgttggggcg aaggttatat ggtgttgcgg cggggttgtt
     8761 ggcggcggcc ttgctgtccg tgaccgtgct gcatattcaa ctcgcccatt tcttcaccag
     8821 cgatccgtat ctcacattct tcgtggtggc tgccgtctat ttcatggtcg ccgcgcacgg
     8881 agactgggcg gcagggccgg ctggcgaagg gcggggaaag tggtatttca tcctggccgc
     8941 gattatggtg gggctggcca tcggctcgaa gttcacggcc gtcttgctca gcctgccgtt
     9001 gctgtggacg gccgcggcta tcggcccttc aagtcgacgc cgttattggg cgctcctcgg
     9061 aatcgtcgtt gccgcccttg cctttgcgct aaccaacccc tttgccgtgc tggattggac
     9121 ctgcaccccg ccgagcaccg gcggtgggga aagcgggttg tcggctacgc tatcgaggtc
     9181 ttgctatatc cagaacattt tgacccagaa cgccatggtc agtggtcggg ctgatttggg
     9241 gtttacccgt cagtacgccg ggacgatacc ctatgtgtac tttggcgaaa tgcttgtccg
     9301 ttgggggtta ggtccgctac ttgggctggt ggctttggcc ggtttggcag tgtcagcatg
     9361 ggatgtcgct cgctcactcc gtggcgtggc gggcatcaat gaggctctgc aacgcttgct
     9421 gcgccaaccg gtcgtcatcg tgctgctgtg ggtcctgcct tatctgcttc tgaccggcgg
     9481 cttctacgtg aagttcctgc gctatatgca gccgatcgct ccctttctga tcctcttcgg
     9541 cgcggctctt ctctggcggg tgcgccggcc gtctgtccgt tatgcccttg ccgggatcgt
     9601 cc
//
LOCUS       DAOMQG010000104         9488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3107_length_9488_cov_10.147779, whole genome shotgun sequence.
ACCESSION   DAOMQG010000104 DAOMQG010000000
VERSION     DAOMQG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_9488_cov_10.147779"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="PLC06_07575"
     CDS             <1..1791
                     /locus_tag="PLC06_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRO90340.1"
                     /translation="WIILKRMIRKYLVVTLLISLLIGLLLANRAAATALEEGSWTEAP
                     IPSGLDLDLSTGAADRAATDPKLDSALAGLVTSRDGHTTDLPGITMIAGRAQVQIVVL
                     PENEAAARLLVSQAGGKVTGAFDGLLQAWLPPEALPALAAESTIEYIQAPNTMVPAET
                     DAIAALTEGVAASGADTWQAAGWRGQGVRIAIIDGGFEGYPARLGSDLPASVTVRNFV
                     DGQTQAEVDGSTRHGTACAEIVHDMAPEARLYLLKINTDVDLNEAVDYAISQGVDIIS
                     TSLTFINVTPGDGTGRFATMAQKARAAGILWTTAAGNYRQTHWSGAFTDADSDGFHEY
                     APGVEVNVFGPGNGTAYSIPAGIALTPSIRWNDWTAVNQDLRLVLLRHNGTSFDIVAT
                     GNNTQSGLSGQRPTERISYLTSGPAAVYGVAIQKVSVNRNIHLHLLTPNRNLDRQIPA
                     MSLGNLADVAAVLTVAAVDAKTPFVRESYSSEGPTNGPGGAPTGGFRKPDLAAFANVS
                     TASYGTRGFNGTSAATPHVAGAAALVLSTYPTAVPGEVQDYLQTTAIDQGLFGPDAQY
                     GYGRLRLGAPLTPGNFPYHSFTPLVVTKTQ"
     gene            1795..4188
                     /locus_tag="PLC06_07580"
     CDS             1795..4188
                     /locus_tag="PLC06_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRO90341.1"
                     /translation="MIKSPAAARVALLLSLLAIPLAYVLTLAKTAVYGDPTEYTFVAN
                     ILGIAHPPGYAFFTLLGKLFQTLIPFGEIPWRMHLLAAMAATAGALFAFGTVRAVARI
                     LFTGLARNMSPVWATASAVFAALSVAFGVNYWQHGIHANPHIVTAAFLSANLFLLTRW
                     AAGGTNNPRWLFAFALSAGLGITHHPLTVISLPAYALFILIARPVILREWRTLLGAIG
                     VALLGLAVWLYFPLRSPMEPAFGPSTMNTLNGFLDHVLARGLSESLPFFGAADIPTRG
                     VVFWSIMRLQYALPVIFLAALGPIWLLVDRKWRPSGGPFPPWKLAVLYGLVFLSNYAF
                     VMSLRAQDIMAYILGLMLIVGMLAGVGLYGLLVMTLARLRLPNAAPVLLAVALFLLGP
                     VLQLTRNAPRISLSRYTEAYDYVNAVFGRFAGTGEGATLLNDWEHMTPIWYAQFVEGR
                     QPDEADVTPVLVSTAQPWLESVFATLPGGPVYLSNYRREIVDAGFRLRPDGVFYQVVE
                     PGDTSIPNELTPLTNEPGHPVEIVAYDLPQRAVAAGGYVPLTLALRAPAGTADYFVPV
                     VHVGDLSFPFTTDSHLTTPQWQPGEIIVERFDFALPHDLPGGAYPVTVSLKNLSADVE
                     LPERYSLGELAVTAQDHPPDYSRLLANFRQRVGLVSATARLGRERRAAVWVEPLPAQP
                     DDTIQLLLQWQSLARAEEGYTVFVHLIDADNRPLVTLDYTPLGGSAPTHLWIPKWLPG
                     QRYLDPYRLQLPADLPPGTYYIEVGLYEMVSKRRLHMADTEGNLIGDRLILGPIAVE"
     gene            4233..5210
                     /locus_tag="PLC06_07585"
     CDS             4233..5210
                     /locus_tag="PLC06_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRO90342.1"
                     /translation="MNKRQRLEAAIAGEPVDRLPVALWRHWPGDDQNAGALAAAHLKW
                     QADYDWDLVKVGPASSYSVVDWGVRDQWEGHIEGTRKTTYLPVQSPADWEALRPLDPS
                     RGMLAEQIEALRLVGEGLRGTTPFIATVFSPLSMAKHLAGNETLLSHLRRDPDTLRHG
                     LEVITESILRFINAARAVGIDGIYYAVQHAAYPLLSRDEFAVFGHPYDLRVLEGAVDL
                     WCNIVHLHGKSVMFDMVADYPMAFLNWHDRDTGISLAEGLSGTAAAASGGVSQWTIHQ
                     EVPEGTLAEVSDARAQTGDRRLLLGTGCVIMTTTPLRNIRALREAAEKR"
     gene            5231..5626
                     /locus_tag="PLC06_07590"
     CDS             5231..5626
                     /locus_tag="PLC06_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HRO90343.1"
                     /translation="MKTIPESHQDLLKADVGILGTIGADGFPQITALWFIVDDDGLIK
                     FYLNNTRQKVKNLQKHPEASFFIVDPAGPYRTVEIRGRVEFTPDPDYAFAPKLTAKYS
                     GTDFRAIDGPGESRLVVTLHPVKVNVWGK"
     gene            complement(5642..6796)
                     /gene="queG"
                     /locus_tag="PLC06_07595"
     CDS             complement(5642..6796)
                     /gene="queG"
                     /locus_tag="PLC06_07595"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRO90344.1"
                     /translation="MSYAAAKLTETLRERAAALGFNRVGVIPAGPARRLDAYLRRIAD
                     GMHGEMGYMARPDRVERRRDPGIILPGVRSFVVVGLDYYTAQLPPALAADPSRGRISN
                     YAWGVDYHDVMTPRLEALAGWLRAQPGAGEVASRVYVDTGAILERDHGETAGLGFTGK
                     NTLLIDPRHGSWFFLGVLLTTQPLDYDPPSEAQPEAPSCGSCTRCLTACPTSAFPKPY
                     VLDARRCISYLTIELKNWIPIELRPLMGNWVYGCDVCQEVCPFNRFARPVGEEVFRAA
                     GWNTAAPPLLDLLALDDDSFARRFANSPIKRIKRARLVRNACVAAGNWGSREAVAPLV
                     RLLGDAGPLVRGHAAWALGRIGGEAAHAALAAAHATETDEDVRRELAAAG"
     gene            6906..7250
                     /locus_tag="PLC06_07600"
     CDS             6906..7250
                     /locus_tag="PLC06_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRO90345.1"
                     /translation="MAESIVNILVEDLKRVELVKISGRIDSSNASQFDSVLKEVAERK
                     HSVVLEMSGVEYISSAGLRAMIALLRECKKQKGDVRLANPSERVVEVLALAGLDSLFE
                     VYDSEAAAVGSF"
     gene            7331..7780
                     /locus_tag="PLC06_07605"
     CDS             7331..7780
                     /locus_tag="PLC06_07605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO90346.1"
                     /translation="MGNEGILTVPGRYNEIKRICEFVAAGAAEAGFDEDDIFHLELCC
                     DEASTNIIEHAYGQEDAGNIEISYHVSDDEFTIILRDNGRAFDPGIVPPPSTITDDNA
                     SAEELASQLRIGGLGLHFIRNLMDEVHFTSDRWLGNQLVMVKKLSVK"
     gene            7790..8146
                     /locus_tag="PLC06_07610"
     CDS             7790..8146
                     /locus_tag="PLC06_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRO90347.1"
                     /translation="MSVWQEKLPHSETWLVGVEGRLDQALNTKLEETLNDLLDNGHYH
                     LVVDLTQATYINSGGLRCLVGAWRRAKANEGSLALCGLNSRLQEIFTMVGFDKVFEIH
                     ENCDTARARGRAKNSS"
     gene            8206..>9488
                     /locus_tag="PLC06_07615"
     CDS             8206..>9488
                     /locus_tag="PLC06_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRO90348.1"
                     /translation="MPSNLPINDQSFIILLLVTAILIAIIFNLLLYLLRRHRSRQILM
                     ERVAEQEALIAAGQALVAAELDIDALCRLIAEQAGQVIDNRTFQVGLFNEGFYDIRYW
                     TIDGEAQVVPQCFDLGVDQKEGVAAGGLVGWVQTTKRPLLVRDFEREMEQLPAQPTYE
                     NMDPPRSALFLPMISGGRTIGLVAAQSQQPEHFSNQDMRRLMILTNQAAAAIANAQLF
                     AQERNRAAHLELMTKIAHVVNAAEQPEEVLELAVDMTCRTFGFQPVTVFGVDHHTQEV
                     IAQASSVPELSPMTATTDRSISLRMPLGQGIVGTAAATHKTVVANSVADDARFISHID
                     SIPPDLTPDTQAEIAIPLLVNDELLGVLDVQSPQIGAFGATERSVLEALAAETATAIY
                     KSRQLAREQELAWITTAQFQVAEAISRHADREGMIA"
BASE COUNT         1819 a         3115 c         2838 g         1716 t
ORIGIN      
        1 tggataatac tcaaacgcat gatcaggaaa tatctcgtcg tcacgctgct catctctctg
       61 ctgatcgggc tgctcctcgc taaccgggcg gccgcgaccg cgctggaaga ggggtcgtgg
      121 actgaggctc cgataccatc aggactggac ttggacctgt cgaccggcgc cgccgatcgt
      181 gccgccaccg accccaagct ggacagcgcg ctggccgggc tggtgacatc aagagacgga
      241 cacacgaccg atctgcccgg aataaccatg atcgcgggtc gagcacaggt acagattgtc
      301 gtcttgccgg aaaacgaggc agccgcacga cttctggtca gtcaggcggg cggcaaggtc
      361 accggcgcat tcgacggcct gctccaggcg tggctgccgc cggaggcatt gccggcgctg
      421 gccgctgaat cgaccattga atacatccag gcccccaaca cgatggtgcc ggccgagacg
      481 gatgctatcg ccgcactgac cgaaggcgtg gccgcgtccg gggccgacac ctggcaggcc
      541 gccggctggc gtggccaggg agtgaggatc gccattatcg atggaggctt cgagggctac
      601 ccggctcgat tgggcagcga tctgcctgca agcgtcacgg tccggaattt tgttgatggg
      661 cagacccagg ccgaggtgga cggctccacg cgtcacggga cagcctgcgc ggagatcgtc
      721 catgacatgg cacccgaggc gcgcctctat ctgctcaaga tcaacaccga tgtcgatctc
      781 aatgaggcgg tcgactatgc catcagccag ggcgtcgata tcatctcgac ctcgctgacg
      841 ttcatcaacg tcacccccgg cgacgggacg ggccgcttcg cgacgatggc ccagaaggcc
      901 cgcgcggccg gcatcctgtg gacgacggcc gcggggaact atcggcaaac tcactggagc
      961 ggggctttca ccgacgccga cagcgacgga ttccatgaat acgcacccgg cgtggaagtc
     1021 aacgtgttcg gccccggcaa tggaacggct tattcgattc ccgccgggat agcgcttacc
     1081 ccgtccatcc gctggaacga ctggacggcc gtcaatcagg atctgcgcct cgtcctgtta
     1141 cgtcataacg gcacatcatt cgacatcgtc gctaccggca ataacaccca aagcgggctt
     1201 tcaggccagc ggccgaccga acgcatctcc tacctcacca gcggcccggc tgcggtttat
     1261 ggtgtcgcca tccagaaagt cagcgtgaac cgcaacatcc atctccacct gctgacgcct
     1321 aaccgcaacc tcgatcggca gatcccggcc atgagtctgg gcaatctggc cgacgtggcc
     1381 gctgtgctga ctgtggccgc cgtcgacgcg aaaacgccgt tcgtccgcga gtcctatagc
     1441 tccgaagggc cgaccaacgg ccccggtggc gcgccgaccg gcggcttccg caagcccgat
     1501 ctggccgctt tcgccaacgt atccacggcc agctacggca cccgcggctt caacggcacc
     1561 tcggcggcga cgccccatgt tgccggcgcg gcggcactgg ttctcagcac ctacccgacg
     1621 gccgtgccgg gcgaagttca ggactacctg caaacgacgg cgattgacca gggcctcttc
     1681 ggccccgacg cccaatacgg ttacggccgc ctgcggctgg gcgctccgct cacgccaggg
     1741 aactttccct atcattcctt cacgccgctt gtggtgacaa aaacccaata gtttatgatc
     1801 aaatctccgg cagcggcgcg cgtcgctctt cttctttcct tgctggcgat ccctctcgcc
     1861 tatgtgctga ccctggcgaa aaccgccgtc tatggggacc cgacggaata caccttcgtc
     1921 gccaacatac tgggcattgc ccacccgccg ggctacgcct tcttcacctt gctgggcaag
     1981 ctgttccaga cgctcattcc cttcggtgaa ataccgtggc ggatgcacct gctggccgcg
     2041 atggcggcca cagccggggc gctcttcgcc ttcggcacag tgcgggcggt ggcccgcatc
     2101 ctgttcaccg gcctcgcgcg gaacatgtcg ccggtctggg cgacggcatc ggccgtgttc
     2161 gccgccttgt ccgtcgcctt cggcgtcaac tactggcaac acggcatcca cgccaacccc
     2221 cacatcgtca cggccgcgtt cctctcggcc aatttgttcc tgctgacgcg ctgggcagca
     2281 ggcggaacaa acaacccccg ttggctcttt gccttcgcgt tgtcggcggg tctgggcata
     2341 acccatcacc ccctgaccgt catctctctt ccggcctacg ccctcttcat cctcatcgcc
     2401 cggccggtca tcctgcgcga atggcgcacc ctgctgggag caatcggggt tgccctgctg
     2461 ggtctggccg tctggctcta cttcccgctg cgcagcccca tggaacccgc cttcggcccg
     2521 tcaacgatga acacccttaa cggcttcctc gaccacgtgc tggctcgcgg cctcagcgag
     2581 agcctgcctt tcttcggcgc ggctgatatt cccactcgcg gcgtggtgtt ctggtcgatc
     2641 atgcggctgc aatacgcgct gccggtgatc tttttggccg ccctggggcc gatctggctg
     2701 ctggtcgacc ggaagtggcg gccgagcggc ggcccgttcc cgccgtggaa gctggcggtg
     2761 ctctacgggc tggtctttct cagcaattac gcctttgtga tgagcctgcg cgctcaggac
     2821 attatggcct acatcctcgg cctcatgctc atcgtcggca tgctggcggg cgtcggcctc
     2881 tatggcctgc tggtcatgac gctcgcccgg ctgcgactgc ccaacgcggc ccccgtcctg
     2941 ctggccgtcg ccctctttct gctggggccg gtattgcaac tgacccgcaa cgccccgcgc
     3001 atctcgttga gccgctacac cgaggcgtat gattatgtaa atgcggtttt cgggcgcttt
     3061 gccggcaccg gcgaaggagc gacgctgctc aacgactggg aacacatgac tcccatctgg
     3121 tatgcgcagt tcgtcgaggg acggcagccg gacgaggccg acgtgacgcc ggttctcgtc
     3181 tcgaccgcgc agccatggct ggagagcgtc ttcgccacgc tgcccggcgg cccggtttac
     3241 ctcagcaact atcgccgcga gatcgtcgac gccggttttc gcctgcggcc ggacggggtg
     3301 ttctatcagg tcgtggagcc gggagacacg agcatcccca acgagctgac acctctgaca
     3361 aacgaaccgg ggcacccggt cgagatcgtt gcttatgatc tgccgcaacg cgcggtggcg
     3421 gccggtggtt acgtgccgtt aaccctggcc ctgcgcgcgc ccgcggggac ggccgactac
     3481 tttgtgcccg tcgtccacgt gggcgacctg tcgttcccct tcacgaccga cagtcacctg
     3541 actacacccc aatggcagcc gggcgaaatt atcgtagaac ggttcgactt cgccctgccc
     3601 cacgacctgc cgggcggagc ctatcccgtc accgtcagcc tcaagaacct gagcgccgac
     3661 gtagaactgc cggaacgcta ttcacttggc gagctggccg tgaccgcgca agaccatccc
     3721 ccggattata gccgcctgtt ggccaacttt cggcagcgcg tcgggctggt gtcggccacg
     3781 gcacgcctcg gccgcgagcg acgcgcggcc gtatgggtcg agccgctgcc ggcgcagccc
     3841 gacgacacga tccaactcct gctgcaatgg caatcgctgg cacgggcgga agaaggctac
     3901 accgtcttcg tccatctcat cgacgccgac aatcggccgc tggtgacgct cgactacacg
     3961 ccgctgggcg gctctgcgcc gacccacctg tggataccca aatggctgcc gggccagcgc
     4021 tatctggacc cctaccgcct gcaattgccc gccgacctgc cccccggcac ctactacatc
     4081 gaggtcggcc tctacgaaat ggtcagcaag cgacgcctgc atatggccga tacagaagga
     4141 aacctgatcg gcgacaggct gattttaggc ccgatcgcgg tcgaatagct atcggaaaac
     4201 ggccgcaagc aacggggaac gggagaaaag taatgaacaa acgccaacga cttgaagcag
     4261 ccatcgccgg ggaaccggtt gatcgactgc cggtggctct ctggcggcat tggccgggtg
     4321 atgaccaaaa cgccggggcc ctcgccgccg cccatctgaa gtggcaagcc gattacgact
     4381 gggacctggt gaaggtcggc cccgccagca gctattccgt cgtcgattgg ggcgtgcgcg
     4441 accaatggga aggccacatc gagggcacgc gcaagaccac ctacctgccg gtgcagtccc
     4501 cggccgattg ggaggccctg cgccctctcg atccgtcgcg cgggatgctg gccgaacaga
     4561 tcgaggcatt gcgcctggtc ggggaggggc tgcgcggcac aacgccgttc atcgccaccg
     4621 tcttctcgcc cttgtcgatg gccaagcatt tggcgggaaa cgagaccctg ctcagccatc
     4681 tgcgccgcga ccccgacact cttcgccacg ggctggaagt gatcaccgag agcatcctgc
     4741 gctttatcaa cgccgcgcga gccgtaggca tcgacggcat ctactatgcc gtccagcacg
     4801 ccgcctaccc attgctcagc cgggacgaat ttgccgtatt cggccacccc tatgatctgc
     4861 gggtgctgga aggtgcggtc gatctgtggt gcaatatcgt tcacctgcac ggcaagtcgg
     4921 tgatgttcga catggtggcc gactacccaa tggcctttct caactggcac gaccgcgaca
     4981 ccggcatcag cctggccgaa gggttgtccg gcacggccgc ggcggccagc ggtggcgtct
     5041 cgcaatggac gatccatcag gaagtgccgg aaggcacgct ggccgaagtg agcgatgcgc
     5101 gcgcccaaac gggcgaccgg cgcttgctcc tcggcacagg ctgtgtcatc atgaccacta
     5161 cgccgctgcg caatattcgc gctctgcgcg aagcagcgga aaagagataa cacagtttaa
     5221 ggagatatcg atgaaaacaa tccctgaatc tcaccaggac ctgctcaagg ccgacgtagg
     5281 catactgggc acaattggcg cggacggctt cccgcaaatc acggcgctct ggttcatcgt
     5341 cgatgacgac ggcctgatca agttctatct caataacacc cgccagaagg tgaagaacct
     5401 gcagaagcat cccgaagcgt cattcttcat cgttgacccg gccggccctt acaggacggt
     5461 ggagattcgc ggccgcgtgg agttcacacc ggacccggat tacgcctttg cccccaagct
     5521 caccgccaaa tatagcggaa ctgatttcag ggctatcgac gggccgggcg agagtcgcct
     5581 ggtcgtcacg ctccatcccg tcaaggtgaa tgtctggggc aagtagacaa gattctcgcg
     5641 tctagcccgc ggcggcaagc tcgcggcgaa catcttcatc cgtttcggtc gcatgcgccg
     5701 ccgccaatgc cgcatgtgcc gcctcgccac caatgcgccc caaagcccat gcggcatggc
     5761 cgcgcaccag cgggccggcg tcacccaaca gcctcacgag cggcgcgacc gcttcccggc
     5821 tgccccagtt gcccgccgcc acacacgcgt tgcgcaccag ccgggcgcgc ttgatacgct
     5881 tgatcgggct gttggcaaac cgccgcgcga acgaatcgtc gtccagcgcc aacagatcga
     5941 gcagcggcgg ggcagcagta ttccagcccg cggcccggaa tacctcctca cccaccggcc
     6001 gggcaaagcg attgaacggg cacacctcct ggcacacgtc acaaccatag acccagttac
     6061 ccattagcgg ccgtaactca atcggaatcc agttcttcag ttcgatcgtc agataggaga
     6121 tgcagcgccg cgcatcgagt acataaggct tcgggaaggc cgacgtgggg caagcggtga
     6181 ggcagcgggt gcatgacccg caggacggcg cttccggttg cgcctccgac ggcggatcat
     6241 aatccagcgg ctgcgtagtc agcaacaccc ccaggaagaa ccacgagcca tggcgcgggt
     6301 caatgagcag cgtgtttttg ccggtgaacc ccagcccggc cgtctcgccg tggtcgcgct
     6361 ccagaatggc cccggtgtcg acgtagacgc ggctggcgac ctcgcccgcg ccaggttgcg
     6421 cacgcagcca accggccaat gcttccaggc gcggggtcat cacgtcgtgg taatcgaccc
     6481 cccaggcata gttggagatg cggccgcgcg acggatcggc ggccaacgcc ggcggcaatt
     6541 gggccgtgta ataatccagc cccacgacga caaaggagcg cacgccgggc aggataatgc
     6601 cgggatcgcg gcggcgctca acgcgatccg ggcgagccat ataccccatc tcgccgtgca
     6661 tgccgtcggc gatgcggcgc agataggcat caagccgccg ggccggaccg gcagggataa
     6721 cgccaacccg gttgaatccc agggcggcgg cccgttcgcg gagcgtctcg gtcagtttag
     6781 cagcagcata agacatggtt caaatatatg ataacacggt catgtggtgt tgacgcattc
     6841 catctatcct atgttataat tcgcttgcta tacatggacc cacaatgcac aggaggcagg
     6901 tgaacatggc tgaatcaatt gttaatatcc ttgttgaaga tctgaagcga gtagaactgg
     6961 tgaagatcag cggccgcatc gacagcagca atgcttccca gtttgatagc gtcctcaagg
     7021 aagttgccga acgcaagcat agtgtcgtgc tggagatgag cggtgttgag tacatcagca
     7081 gcgcgggtct gcgagccatg atcgccctgc tacgcgagtg caagaagcaa aaaggcgacg
     7141 ttcgcctggc caatccatcg gaacgggttg ttgaagtgct ggctctggct gggctggatt
     7201 cgctatttga agtctatgat agcgaagcgg cagccgtcgg cagcttctag tcacctggcc
     7261 tgtcgttgaa cttgggtcta ctttgtccct gccgatgaca gggtagttga gtgcgcctta
     7321 attcctgaat atgggcaacg aaggaatcct gaccgtcccg gggcgataca atgagatcaa
     7381 gcggatctgt gagttcgtcg ctgccggagc ggccgaggcc ggattcgacg aggacgacat
     7441 cttccatctg gaattgtgct gcgatgaggc atcgaccaat atcatcgagc acgcctatgg
     7501 ccaggaggac gcggggaata tcgagatcag ttaccatgtc agcgacgatg agttcaccat
     7561 tatcttgcgc gacaacggcc gagcgttcga tcccggcata gtcccccctc cttcgaccat
     7621 taccgacgac aacgcatcgg ccgaagagct ggcgagccaa ttacgcatcg gcggcctggg
     7681 cctgcacttc atccgcaatc tcatggacga ggtccatttc acctccgacc gatggctggg
     7741 caatcagctt gtcatggtga aaaagctcag cgtcaaataa ggaagcctaa tgagtgtctg
     7801 gcaagagaaa ctcccccata gcgagacctg gctggttgga gtcgaaggac gactcgacca
     7861 ggctcttaat acaaaactgg aagaaacgct caacgatctt ctcgacaacg gtcactatca
     7921 cctcgtcgtg gacctgacgc aagcgaccta catcaacagc ggtgggctgc gctgcctggt
     7981 cggggcctgg cggcgagcca aagccaacga ggggtcgctg gctctttgcg ggttgaacag
     8041 ccggttgcaa gagattttca ctatggtcgg tttcgataaa gtcttcgaga tccacgagaa
     8101 ttgcgacacg gcgcgcgccc gcggccgcgc gaagaacagc tcctgacgtc ggatatgtag
     8161 ccccgcttct catccgtgaa gccgggcagg tcccccatcc cggccatgcc ctccaatctc
     8221 cccatcaatg accagagctt catcatcctg cttctggtta ccgcaatcct gatcgcgatc
     8281 atcttcaacc tgttgcttta tctgctgcga cgccatcggt cgcgacagat tctcatggag
     8341 cgggtggccg agcaggaggc gctgatcgct gccgggcagg cgctcgtcgc tgccgaactt
     8401 gacatcgacg cgctctgccg cctgatcgcg gaacaagcag gtcaggtcat tgataatcgc
     8461 acctttcagg tgggcctgtt taacgagggg ttctacgata tccgctattg gacgatcgac
     8521 ggcgaggccc aggtcgttcc ccaatgtttt gatctaggcg ttgatcagaa ggaaggcgtg
     8581 gcggccggcg gcctggtcgg ctgggtacag acgacgaaac gcccgctgct cgtgcgagat
     8641 ttcgagcgcg agatggagca gctgccggct caacccacct atgaaaacat ggacccgccg
     8701 cgctccgctc tgtttctgcc gatgatcagt ggcggccgca ccatcggtct cgtcgccgct
     8761 cagagccagc agcccgaaca tttcagcaat caggacatgc gccggttgat gatcctgacc
     8821 aatcaggcgg ccgcagcgat cgccaacgcc caactcttcg cgcaagagcg caatcgcgcc
     8881 gcccatctgg aactgatgac caaaatcgcc catgtggtga atgccgccga acagccggaa
     8941 gaagtcctgg agctggccgt cgatatgact tgccgcacgt tcggattcca gccggtgacc
     9001 gtcttcggcg tcgaccatca tacccaggaa gtcatcgccc aggcgagcag tgtgccggaa
     9061 cttagcccga tgacggcaac aaccgaccgc tcgatctcat tgcggatgcc gcttgggcag
     9121 gggattgtcg gcacggccgc cgccacgcat aaaacagtgg tcgccaacag cgtggccgat
     9181 gacgcgcgct ttatcagcca tattgacagc atcccccccg acctgacgcc tgatacccag
     9241 gccgagatcg ccatcccgct gctcgtcaac gatgagctgc tgggcgttct ggatgttcaa
     9301 agcccccaga tcggcgcgtt tggcgctacc gagcgttccg tcctcgaagc attggcggcc
     9361 gaaacagcca ccgccatcta caaatcccgg caactggccc gcgaacagga gctggcctgg
     9421 atcacgaccg cccaattcca ggtggcggaa gccatcagcc gccacgccga tcgcgaaggg
     9481 atgatcgc
//
LOCUS       DAOMQG010000105         9443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3124_length_9443_cov_9.421282, whole genome shotgun sequence.
ACCESSION   DAOMQG010000105 DAOMQG010000000
VERSION     DAOMQG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3124_length_9443_cov_9.421282"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLC06_07620"
     CDS             complement(<1..242)
                     /locus_tag="PLC06_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90349.1"
                     /translation="MVNELWGLGFSGIAWPDSALGYGLWAFSIGLLAWSLIRSGVLSA
                     MRGLNRDQWALFVALCGASLLFSQLFPLTIPWSNSLL"
     gene            372..863
                     /locus_tag="PLC06_07625"
     CDS             372..863
                     /locus_tag="PLC06_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90350.1"
                     /translation="MDTRRRTYAILVGIILLTVPCYLSGIVLLSRAPNRGGSPTLEAN
                     TATPTASPTPTRGGTQLPPAIISLTPPGSPTPQATITSSITPTPTDTSTPTDTPTLTP
                     TPTDTSTATPTPTETPTITPTPTETPTETPTPTETPTETVTPTPAATDNGMTATPDAV
                     GTP"
     gene            884..2020
                     /locus_tag="PLC06_07630"
     CDS             884..2020
                     /locus_tag="PLC06_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO90351.1"
                     /translation="METFDLLKQLTEAPGPTGKEEGAAAVIEPLWRPLVDEIVADRVG
                     SLVGIKRGAGPMERPRPSILLAAHLDELGLMVSRIVEHGGYGFLRVTGLGGVDRRHLF
                     GQTAVVHGVKPLTGVLGGLPYSYLPEQERDKAFSYETLVLDVGLPVEQVRELVSIGDF
                     ISFRQPLRKLGNGRVAGKALDNRSSVAAVTICLEYLQGRRHMWDVIAVATSQEETTYL
                     GAYTSAYAQRPDAAVVIDVTHAKGPGSSESELAELGGGPVLGIGANVHPGLFKALRDA
                     AEALEMKVGIEPHAAGSGTDAYAIQVAREGIPTGLIGIPLRYMHTMVETIDMADVERV
                     GRLLAEMIARLDDHFMDGIREDMMRPHKPVGNGAEQNATKDGNA"
     gene            2017..3060
                     /locus_tag="PLC06_07635"
     CDS             2017..3060
                     /locus_tag="PLC06_07635"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO90352.1"
                     /translation="MNDLLKQLTEAIGVSGAEKEVRLLIRDLIADYVDEWSVDAMGNL
                     VAIRRGTGQYDMRVLIDAHMDEVGLLITDIDSGGMLKFTNVGGFDDRALLGKVVQVGP
                     DKITGVIGARAIHLLKSSEYSKVVKMDAMRIDIGAKSKEAASAKVKVGDYAAFVTEYE
                     ELDSDRTAIGKAFDNRAGCAALIELLRGERYPFDLVAAFTVQEEVGLRGARIAAFNAR
                     ADAALVLECTPAYDLPNEKDVSPNVAMGKGVSVYIMDSRTIQDPRFVAHIMATGDAQG
                     IPYQVRQPGGGGTNTGMIQRAGAGIPAATLAVPGRYAHTPTMMISLDDYHNIVRLADA
                     TLRTLTPDILRRA"
     gene            3091..4095
                     /locus_tag="PLC06_07640"
     CDS             3091..4095
                     /locus_tag="PLC06_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRO90353.1"
                     /translation="MNDLIRTLTEAFGPSGFEDAMRDLIRPLVEPYADEITVDSLGNL
                     IVHKNAAAGNKNPQKVMIAAHMDEIGVMVSHITEKGFLRFTNIGGVFSHTLLGGRVRF
                     ANGTIGVIGSDRSDDRNAIHPLNKHFIDVGATSREDCPVRVGDSAQFYRPFEVRGRHL
                     TAKSMDDRIGCVVAIEALRRLPEAVAHDLYFVFSVQEEVGTRGAEAAANGIMPDVSIA
                     LDVTLTGDVPEARPMDVSLGDGPAIKVKDSGMIAHAGLVRLMRRRAEAAGIEYQLEVL
                     DGGSTDARAMQLAGPGSAAGCISIPCRYVHTPSETVDAGDVEGAIRLLVAVLQGEMDY
                     "
     gene            complement(4114..5055)
                     /locus_tag="PLC06_07645"
     CDS             complement(4114..5055)
                     /locus_tag="PLC06_07645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRO90354.1"
                     /translation="MSLYNQTVEIILEAEGERLDRALTELLLGLSRMQWQRLIREGHV
                     TVDGQRAKASYRIIGGETLIASIPEVVDAEVTAEDIPLDIRYEDKDLIVVNKPAGMVV
                     HPSLGHAGGTLVNALLAHCPDLEGVGGERRPGIVHRLDKDTSGLIIAAKHDQALWELQ
                     NQFKQRSVAKVYLTLVNGSLQPPEAVIDAPIGRDPRNRQKMAVVTKAGSDARPAQTAF
                     RALAYHPDYTYVECRPRTGRKHQIRVHMAYIGFPIAGDPIYGRRKDKLGLKRHFLHAA
                     ELTFRRPSDDEELHLTSELPDDLARVLAQLSGPALEN"
     gene            complement(5115..>5519)
                     /gene="lspA"
                     /locus_tag="PLC06_07650"
     CDS             complement(5115..>5519)
                     /gene="lspA"
                     /locus_tag="PLC06_07650"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRO90355.1"
                     /translation="PGLENLFRITHTANTGAVFGLFQGTGMFFAGLAIIVAVAILYIN
                     YTLPGKQWLLRLALGLQLGGALGNMIDRFRQGHVTDFIDIGPWYIFNVADMALVGGVI
                     LFGIVLIRDERNTRKNAPIPVNSLDNQERLDL"
     assembly_gap    5520..5619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5620..6252)
                     /locus_tag="PLC06_07655"
     CDS             complement(<5620..6252)
                     /locus_tag="PLC06_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO90356.1"
                     /translation="MSLRSTIILYIFDLLYTHFAWAYDAVSWAVSRGRWQSWGRAALP
                     FLIGPEVLELGHGPGHLLATLEADGWMATGIDISSQMGRLAKKRLAEKELPVCLARGR
                     AQDLPFRNGTFDSVVATFPAPYIVDPETARAIREVLRPGGRLVIVLGGTPIGKSSIKR
                     LLGWLFGSTRKRIIHISEPSSRSGAWGRIYEPAGFAVTIYHVVQPNYVVIV"
     gene            complement(6252..8111)
                     /locus_tag="PLC06_07660"
     CDS             complement(6252..8111)
                     /locus_tag="PLC06_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRO90357.1"
                     /translation="MTVPQWLASIHHDGSEKYVSILYPRLGDTVRLRLRVAHDAPVNR
                     VILRTFPDGEQAFKVMERTVTQAPAQWWEIDLPINEPLTHYRFVIDSADGLWHYTAAG
                     PSRFIPLDTTDFRILADYQSPAWLSSAVFYQIFPDRFANGDPANDPRPDDYEYRGQFP
                     KTFPWETPPGEGQFFPLVFYGGDLAGISSRLNYLADLGVNALYLNPIFYALSNHKYDV
                     ADYERVDPHFGGNEALASLRHALSARGMRYILDIVPNHCGYWHPWFQTALTDVDAPEA
                     EFFTFNRHPDDYATWLGVWVLPKLNYRSHELRRRIYQGAEAVFRHWLRPPYSADGWRV
                     DVANMLGRQGATQIGLEISRGIRQSVKGTRPDAYLMGENFFDATGQLQGDQWDGVMNY
                     GGFTQPVWYWLCGFTQGAHGFSETMNAPQWPAEAMVEMWQSRMAAVPWVIALQQYNLL
                     GSHDTPRIRTLVGGNDALHRLATALLLTFPGVPGLYYGDEIGMTDVPDLGARGCMIWD
                     ESRQDRDLYGYHKRLIHFRRSSAALQRGGFQMLLVEPDTVAYQRESIDERVIVVAHRA
                     EMPRAPGPLPLSHTGVADGTQFIDLMSGTEFITQEGMLSLPALPQGVLILLEA"
     gene            complement(8190..8645)
                     /locus_tag="PLC06_07665"
     CDS             complement(8190..8645)
                     /locus_tag="PLC06_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRO90358.1"
                     /translation="MYKTILVPLDGSELAETVLPHVEELARLFGSTVVLLSVLELPHL
                     VGLPKGDLFDALPQMTPAEVNSHLTEVRRYLDEKAGQLKQKGVEARVLVEYGPVVVTI
                     MRAARQEDAGLIAMASHGRGGMADVYYGSVAAGVLQRIDRPLLIVRAQG"
     gene            complement(8711..9040)
                     /locus_tag="PLC06_07670"
     CDS             complement(8711..9040)
                     /locus_tag="PLC06_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRO90359.1"
                     /translation="MNTLMMALAILGMGLITYAIRVSLFLLPDRVSLPPWLLRSLRYV
                     PAAVLTAIIFPELLLPQGTIDFSLGNERLLAGVCAILIAWRTRNVFLTVVLGLIILWL
                     LQTYNPW"
     gene            complement(9037..>9443)
                     /locus_tag="PLC06_07675"
     CDS             complement(9037..>9443)
                     /locus_tag="PLC06_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO90360.1"
                     /translation="TDEAYAVTIIHYTEGRTPPATRHWFFLGAGMALWVTWQTTTAIG
                     IFLGASIPATWSLDFALALTFIGLVVPTLRNRPHTAAALTAGLVAVLAASWPYKLGLI
                     AAALSGILIGTVLESFDRPAPELLNLDRESNQ"
BASE COUNT         1977 a         2689 c         2844 g         1833 t
ORIGIN      
        1 agcagggagt ttgaccatgg aatagtcagc ggaaataact ggctaaagag gagcgaagcg
       61 ccacagaggg ccacgaacaa tgcccattgg tcacgattaa ggccccgcat agccgagagt
      121 acgcccgatc ggatgagcga ccatgccaat aagccgattg agaaggccca taagccataa
      181 cccagcgccg aatccggcca ggcaatcccg gaaaaaccga ggccccataa ttcgtttacc
      241 attcgtgcgc ttgcccttgc aatctatcga gccttttcct ttggatgttc cctacattat
      301 gtctgcttca acagtataac agtgccgtcc taatttgtca tttggagggg agttcattat
      361 gatacgatct catggataca cgccgccgga catatgccat tctggttggc atcatccttc
      421 tcaccgtgcc atgttacctg tccgggatcg tattattgtc acgcgcgccg aaccggggcg
      481 gttctcccac ccttgaagca aatacggcaa cgccaactgc gtcgcccaca ccaacccgtg
      541 gcgggacgca gctgccgccg gcaatcatct cgcttacgcc gcccggctca ccgacgcctc
      601 aggccactat tacatcctca atcacaccaa cgccgaccga cacatcgacg ccaaccgata
      661 cgccgacgtt gaccccgacg ccgacggata cgtccacggc aactccgact ccaacggaaa
      721 cgccgactat aaccccgacg ccgacggaaa cgcccaccga gacgccaact cctacggaga
      781 cgccaaccga gacggtgact ccgaccccgg cggccacaga caatgggatg acggccacgc
      841 ctgacgccgt tgggactccc tgaaattcgc aaggatttac gccatggaaa cgtttgattt
      901 gctgaagcaa ctgaccgaag cgcccggccc aaccggcaag gaagaaggtg cggccgcagt
      961 tattgagccg ctatggcggc cgctggtaga cgagatcgtc gccgatcggg ttggaagcct
     1021 ggtgggcata aagcgtggtg ccggcccaat ggagcggcca cgccccagca ttttgcttgc
     1081 cgcccacctg gatgagttgg gtctgatggt ctcgcgcatc gtggagcatg gcgggtatgg
     1141 ctttctccgt gtgacggggc ttggtggcgt cgatcggcga caccttttcg gccagacggc
     1201 cgtggtccat ggcgtaaagc cattgacggg tgttcttggc ggactgccct attcctattt
     1261 gccggagcaa gaacgcgaca aagccttcag ctatgagacc ctggttctgg atgtgggcct
     1321 gcccgtcgag caggtacgcg agctggtgtc gattggcgat ttcatctcgt ttcgccagcc
     1381 attgcgaaag ctgggcaacg gccgtgtagc cggcaaggcg ctcgataatc gctcgtccgt
     1441 ggccgcggtc acgatttgtc tggaatatct tcaggggcgc cgtcacatgt gggatgtcat
     1501 cgccgtagct acctcgcagg aagagacgac ctacctcggt gcatatacca gtgcttatgc
     1561 ccagcggccg gatgcggctg tggtcatcga tgtgacccat gccaaggggc cgggttcgag
     1621 tgagagcgag ttggccgaat tgggtggcgg ccctgtcctg gggatcggcg ccaacgtcca
     1681 ccccggccta ttcaaggctt tgcgggatgc ggccgaagcg ctggaaatga aggtcgggat
     1741 cgagccgcac gcagccggca gcggcacgga tgcctatgcc atccaggtcg cgcgcgaagg
     1801 tattcccacc ggtttgattg gaatcccctt gcgctatatg cacacgatgg tcgaaacgat
     1861 tgacatggct gacgtggagc gcgtgggccg gttgctggcg gagatgatcg ctcgtctgga
     1921 tgatcatttc atggacggca tacgcgagga tatgatgcgg ccgcacaaac ccgtcgggaa
     1981 cggcgccgag caaaatgcaa ccaaggacgg taatgcatga atgacctgtt gaaacaactt
     2041 accgaggcga tcggtgtctc cggcgcggag aaagaggtac gcctgctaat tcgcgatttg
     2101 attgccgatt acgtcgacga gtggtcggtc gatgcgatgg gcaatctggt ggcgatcagg
     2161 cgtggcaccg gacaatacga catgcgcgta ctcatcgatg ctcacatgga cgaagtcggc
     2221 ttgcttatca ccgatatcga cagcggcggc atgctcaagt tcacgaacgt aggtggcttt
     2281 gacgatcgcg cgctgctggg caaggtcgtc caggtggggc cggacaagat aaccggggtg
     2341 attggcgcgc gcgcaatcca tctgctgaag agcagcgaat actccaaggt ggtcaagatg
     2401 gacgccatgc gcatcgacat cggcgcgaaa agcaaggaag ccgcttcggc caaggtgaag
     2461 gtgggcgatt atgcggcctt tgtcaccgaa tatgaagagc ttgatagcga ccgaacggcc
     2521 atcggcaagg ctttcgacaa tcgggcgggg tgcgcagcgc tgattgaact gctccgtggc
     2581 gagcgctatc cctttgatct ggtggcggcc ttcaccgttc aggaagaggt cggtctgcgt
     2641 ggggccagga tcgccgcgtt caatgccagg gccgatgccg cactggtgtt ggaatgcact
     2701 cctgcctatg atctgccaaa cgagaaagac gtcagcccca acgtcgctat gggcaaaggc
     2761 gtttcggtct atatcatgga ttctcgaact atccaggacc ccagattcgt agcccatatc
     2821 atggctacgg gcgacgctca gggcattccc taccaggttc gccagccggg cggcggcggc
     2881 acgaacacgg gcatgatcca gcgcgccggt gccgggatcc ctgcggctac gttggctgtg
     2941 cccggccgtt atgcccacac gccgacgatg atgatcagcc ttgatgacta tcacaatatc
     3001 gttcgcctgg ccgatgctac cttgcgaacg ttgacccccg atattttgcg ccgcgcctga
     3061 gcggttggaa catgagagat ggaaaacgct atgaatgacc tgattcgcac gctcacggaa
     3121 gcctttggcc cttccggttt tgaagatgcc atgcgcgacc tgattcgtcc gctggtggag
     3181 ccttatgccg atgaaatcac tgtggactcg ctcggcaacc tgatcgttca caaaaatgcc
     3241 gcggccggca ataaaaaccc gcaaaaggtg atgatcgccg cgcacatgga tgagattggt
     3301 gtcatggtga gccatattac ggagaagggc tttctgcgct tcaccaatat cggtggggtt
     3361 tttagccata ccctgctcgg cggccgcgtc cgtttcgcca acggcacgat cggcgttatt
     3421 ggctccgacc ggagcgatga tcggaatgcg atccatccgc tcaacaagca cttcatcgac
     3481 gttggcgcga ccagtcgtga agattgcccg gtcagggtgg gcgactcggc ccagttttac
     3541 cggcccttcg aagttcgcgg ccgccatctg acggccaaga gcatggatga ccgtatcggc
     3601 tgcgttgtgg cgattgaagc gctacgtcgc ctgccggaag ccgtggctca tgatctgtat
     3661 tttgtcttca gcgtacagga agaagtgggc acacgcggcg cggaagcggc cgccaacggc
     3721 atcatgcccg atgtgagcat tgcccttgac gtgaccttga ccggcgacgt accggaggcg
     3781 cggccgatgg acgtgagctt gggggatggt ccggcgatta aggttaagga cagcggcatg
     3841 atcgcccacg ccggattggt gcgcctgatg cgtcgtcgcg ctgaagcggc gggaatcgaa
     3901 tatcagctgg aagtgcttga tggcggctcg accgacgccc gggccatgca attggctggg
     3961 ccaggcagcg ccgccgggtg catctcgatc ccttgccgat acgtccacac gccgagcgag
     4021 accgtggatg ccggcgatgt ggagggcgcg attcgcctgt tggtggccgt actgcaaggt
     4081 gagatggatt actaggcaga aacggctaaa gccttaattt tcgagggctg ggccggatag
     4141 ttgcgccaac acgcgagcga gatcgtcggg taattcagag gtgaggtgca gctcctcgtc
     4201 gtccgacggc cgccgaaaag tcagttcggc cgcgtggagg aagtggcgct ttaatcccag
     4261 cttgtctttc cggcggccgt agatagggtc gcccgcaata ggaaagccga tataggccat
     4321 atggacgcgt atctgatgct tgcggccggt tcggggacgg cattccacat aggtgtaatc
     4381 cggatggtag gctagcgcgc ggaaggccgt ttgtgcggga cgcgcgtcgc tgcctgcctt
     4441 cgtaacgacg gccatctttt gtcgattgcg cggatcgcgg ccgattggcg cgtcgataac
     4501 tgcttccggc ggttgaagcg agccattgac gagggtcagg tagacttttg ccacactgcg
     4561 ttgcttaaat tgattctgta gctcccacaa cgcctgatcg tgtttggcgg cgatgatcag
     4621 gccggacgtg tccttgtcca ggcgatggac aattccgggg cggcgttctc cgcccacgcc
     4681 ttccaggtcg gggcagtgtg ccagcagcgc gttgaccagc gtgccacctg cgtgacccag
     4741 cgatgggtgc acgaccattc ccgcaggttt gttgacgacg ataaggtcct tatcctcata
     4801 ccggatgtcc aacgggatat cctcggccgt cacttccgcg tcgaccactt ccgggatgga
     4861 tgctatcaac gtctctccac cgatgatccg atagctggcc ttggcgcgct ggccgtccac
     4921 ggtgacatgc ccctcacgga tcaggcgttg ccactgcatg cgcgagaggc ctagcaacag
     4981 ttccgtcaag gctcggtcca aacgctcgcc ttctgcttcc aggattattt caactgtttg
     5041 gttatacaag gacatgattg atttgggagg gtcgcaaatg agtttgacgg cgaggaggat
     5101 ggatcgccgc gcgcctaaag atcgagtcgc tcctggttgt cgagcgaatt gaccgggatt
     5161 ggcgcgtttt tccgtgtgtt tctctcgtca cgaatcagca cgatcccgaa caaaatgacg
     5221 ccgccgacca gagccatatc ggccacgttg aaaatatacc aggggccgat gtcaatgaag
     5281 tcagtcacat ggccctgacg aaaacgatcg atcatattgc ccaacgcgcc gcccaattgc
     5341 aggcctaatg ccaaccgcag caaccattgt ttgcccggca gcgtgtagtt gatgtagagg
     5401 atggccacgg ccacgataat ggccagcccg gcgaagaaca tgcctgtccc ctggaacagg
     5461 ccgaaaacag ctccggtatt ggccgtgtgg gtaattcgga acaggttctc aagtcctggn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc actatgacga cgtaattcgg
     5641 ttggacaaca tgataaatgg tcacggcgaa ccccgccggt tcgtagattc ttccccaggc
     5701 tcctgacctg gatgatggct cgctgatatg gatgatccgc ttcctggtgc tcccgaagag
     5761 ccagcccaac agccgtttta tcgagctttt accgatcggc gttcccccca acacgatgac
     5821 cagacgaccg ccgggtcgca agacttcccg gatcgctcgc gctgtctcgg ggtcgacgat
     5881 atagggcgcg ggaaatgtgg cgacgacgct atcaaaagtt ccatttcgga agggtagatc
     5941 ctgcgcccgg ccgcgggcga gacagacagg aagctccttt tcggctaagc gcttttttgc
     6001 cagccggccc atttgtgatg agatgtcaat ccctgtcgcc atccatccgt cagcttccag
     6061 cgttgccagc aagtgacccg gtccgtgacc taattccaaa acctcggggc cgatcaagaa
     6121 gggtagcgcg gcccttcccc atgattgcca gcggccgcgc gaaacggccc aactgacggc
     6181 atcataggcc cacgcgaagt gcgtgtaaag caaatcgaag atatacagga tgattgtgct
     6241 tcggagtgac attacgcttc cagcaggatc aagacgcctt gcggcagagc ggggagcgat
     6301 agcatgcctt cctgggtgat gaattcagtc ccactcatca gatcgatgaa ctgtgttccg
     6361 tcagccacgc ctgtatggct gaggggtaat gggcctggag cgcgtggcat ttcagcgcgg
     6421 tgagcgacga cgatgacgcg ctcatcaatg ctttcacgct ggtaagcgac ggtatccggc
     6481 tcaaccaaca gcatctggaa ccccccgcgc tgtagggccg cggaactccg tcgaaaatgg
     6541 atcagccgct tgtgatagcc gtagagatcg cgatcctgtc gcgactcatc ccaaatcatg
     6601 caaccccgtg cgccaaggtc aggaacgtcg gtcatgccga tctcgtctcc gtagtaaagg
     6661 ccgggtacgc cgggaaatgt gagcagtaaa gccgtcgcca gccgatgcaa ggcatcattt
     6721 ccgccaacaa gagttcggat tcgcggggtg tcgtggctgc cgagcaggtt gtattgctgc
     6781 aaggcgataa cccacggcac ggccgccatc cggctttgcc acatttcgac cattgcctcg
     6841 gccggccact gtggcgcgtt catcgtctcg ctaaatccgt gtgctccctg ggtgaagccg
     6901 cacagccaat accataccgg ctgcgtgaac cccccgtagt tcatcacgcc gtcccattga
     6961 tcgccctgga gctgccccgt ggcgtcgaag aaattctcac ccatcaggta ggcgtccggg
     7021 cgggttccct tcaccgactg gcggatgcca cggctgatct ccaggccgat ttgtgtcgct
     7081 ccctggcggc cgagcatgtt ggctacgtcg acccgccagc cgtcagcgga gtagggcggc
     7141 cgaagccaat gacggaaaac ggcttccgcc ccctgataga tgcggcgacg cagttcgtgg
     7201 ctacgatagt tcagcttggg caaaacccat acgccaagcc acgtcgcata gtcatcaggg
     7261 tgacgattga aggtgaagaa ttcagcctcg ggtgcgtcca cgtcagtcag cgctgtctgg
     7321 aaccaggggt gccagtagcc gcaatggttt gggacgatat cgagaatgta gcgcatcccg
     7381 cgggcgctca gcgcgtgacg caacgacgcc agcgcctcat tgccgccaaa atgcgggtcc
     7441 acgcgctcat aatccgccac gtcgtactta tggtttgaga gggcgtaaaa aatcgggttc
     7501 aggtagaggg cattaacgcc cagatcagcc agataattca gccgagagct gatgccggcc
     7561 agatcgcccc cgtagaagac gagggggaag aattggcctt cgccgggtgg agtctcccag
     7621 gggaacgttt tagggaattg gcctcggtac tcataatcat ccggtcgcgg atcattggca
     7681 ggatcgccgt tggcgaaccg gtcggggaaa atctgataga acacggccga acttagccat
     7741 gccggtgatt ggtaatccgc caaaatgcgg aagtcggtcg tgtcgagcgg tatgaagcgg
     7801 gaaggcccgg cagcggtgta atgccacagc ccatcggccg agtcgatgac gaaccggtaa
     7861 tgggtcagcg gttcgttgat gggcagatcg atctcccacc actgggcggg agcctgtgtc
     7921 accgttcgct ccatcacctt gaaggcttgc tcgccgtcag ggaaagtgcg caaaatcacg
     7981 cggttcaccg gcgcgtcatg ggccacgcgc agacgaagcc ggacagtatc acccagtcgt
     8041 gggtagagga tggaaacgta cttttcggat ccatcgtggt gaatagaggc aagccattgt
     8101 gggacggtca tagtcgtttt agttggcaaa acggatgaag aatgaaccct tgctgaaagg
     8161 gcatgaacga ttggaggatc accaaccgat tagccttggg cgcgaacgat cagaagaggg
     8221 cgatcgattc gctgcaaaac gcccgcggca acgctgccgt agtagacatc ggccatgccg
     8281 ccccgcccgt ggctggccat ggcgattagt ccggcatctt cctgtcgggc agcgcgcatg
     8341 atcgtgacga ccaccggccc atattcgacc agaacacgag cctcaacgcc cttctgtttc
     8401 aattggccgg ccttctcatc cagatatcgg cgaacttcag ttagatggct gttcacttcc
     8461 gccggggtca tctgcggcag cgcatcgaag agatcgccct tgggcagccc gaccagatgg
     8521 ggaagctcca gcacgctcaa cagcacgacc gtggagccga agagccgcgc cagttcctca
     8581 acgtgaggca gaaccgtctc ggccaactcc gagccatcga gggggaccag gatggttttg
     8641 tacatggtat ctcctgtgtc atctaccggt aggcccctac tttacgcgtt ccggccggat
     8701 tagtaaagag ttaccagggg ttatacgtct ggagcagcca gagaatgatc aggccgagga
     8761 caaccgtcag gaacacattg cgcgtgcgcc aggcgatgag aatggcgcac accccggcaa
     8821 ggaggcgctc gtttcccagg gagaagtcaa tagtcccctg ggggagcagc aactccggga
     8881 atatgatggc ggtcaggacg gccgcaggca catagcgcag cgatctcaag agccaggggg
     8941 gcaacgatac ccgatccggc agcaggaaga gcgacacgcg aatggcgtag gtgatcaaac
     9001 ccatccccag aatagcgaga gccatcatca atgtgttcat tggttactct ctcggtcgag
     9061 gttaaggagt tccggagcag gtcgatcaaa gctttccaat acggtgccaa tcaggatccc
     9121 cgacagggcc gcagcgatca gtcccaactt ataaggccat gaggcggcca gaacagccac
     9181 cagccccgcc gtcagcgctg ccgccgtatg tggccgattg cgcagagtgg gcacgaccaa
     9241 cccgatgaac gtcaacgcca gggcaaaatc aagcgaccag gtggccggta tgcttgctcc
     9301 caggaagata ccgatcgctg ttgttgtctg ccaggtaacc catagtgcca taccggcgcc
     9361 cagaaagaac cagtggcgcg tcgccggggg tgtgcggcct tcggtgtagt gaatgatcgt
     9421 cacggcatag gcctcgtcgg tca
//
LOCUS       DAOMQG010000106         9436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3131_length_9436_cov_10.894574, whole genome shotgun sequence.
ACCESSION   DAOMQG010000106 DAOMQG010000000
VERSION     DAOMQG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_9436_cov_10.894574"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLC06_07680"
     CDS             <1..1114
                     /locus_tag="PLC06_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO90361.1"
                     /translation="FSDLFKVRADFSDTMPRDAANEMLYAAFIASRCQEEKLRPFGRD
                     AVAIIIEEGARLAEHQRKLSTRFGAIADLARESSYWAGIGGHEVVTADDVREALKERI
                     HRASQDAEQVREDILEGTIFIATQGEAVGQVNSLSVREVGDFIYGHPGRISARTFMGD
                     NGLLHIERETDMDGPIHEKGVLILNGYLGGTYAQHQPLSLNASLTFEQTYGGIDGDSA
                     SSTELYALLSSLSGYPIRQGISVTGSVNQRGEIQPIGSVNEKIEGFFDICHARGLTGD
                     QGVIIPATNVVNLMLREDVVAAVCAGQFHVWPITSINEGIELLMGRPAGEADEAGNYP
                     EGTVHHAVKKRLMELAMELKSFGDDRGHDQSEDDED"
     gene            complement(1116..1652)
                     /locus_tag="PLC06_07685"
     CDS             complement(1116..1652)
                     /locus_tag="PLC06_07685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRO90362.1"
                     /translation="MEILESRIIWDGKWRVRVNTVRMDDGADYERAMVEHPGAVVLAP
                     LVTTADGAPALLMLRQYRPTLGTLLFELPAGTREGDEPWLQCAQRELREETGYRAAEM
                     ISLGEVWAAPGMSNERMALFVATGLTADPLPMDIDEEITVETWPLEELVAMAIDGRLE
                     DAKSVIAILRLAHHLSQQ"
     gene            complement(1652..2143)
                     /locus_tag="PLC06_07690"
     CDS             complement(1652..2143)
                     /locus_tag="PLC06_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90363.1"
                     /translation="MKRSTLLLLLTSMLVLFGLTGCEIPAPESQNFLATSTAAALATA
                     PAPGTLAPGTIVLPPVGASPTPAPLMTADPNQPLPTDATPTPDPAAQPGGETTQETAP
                     TEPTAETSSSGEIIHTVQPGDNLYRIGLRYGFTFQELAQYNGITNPDRLEVGQQIRIP
                     PKP"
     gene            2142..2270
                     /locus_tag="PLC06_07695"
     CDS             2142..2270
                     /locus_tag="PLC06_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90364.1"
                     /translation="MRELLQCSNRRAHGVRRVNYRGMASSIIHADDELQCRSRDGM"
     gene            2424..3569
                     /locus_tag="PLC06_07700"
     CDS             2424..3569
                     /locus_tag="PLC06_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO90365.1"
                     /translation="MTRAHIGINAHLLATATGYRRAGIHHYIHQIVRHLPPTTVTRYT
                     VYTRLTDGWEHRSDMGMVGTRLPTENRAARIVWEQAVWPFQARRDELTLMHSMAFAMP
                     RLAPCPVVVTIYDLSFVENPEAFPAAQRRYLQHETAYSCAHAARLVAISESGRRDIHR
                     HFDVPLERIDVVTPGVDDRYRPLPADRVAAFRREKGLPEIFILHVGTLQPRKNIPTLL
                     DALARLERPDVALVLVGGRGWIYEEIFARVQALGLADRVRFAGYVGDEELPLWYNAAA
                     VLAFPSFYEGFGMPVADALACGTPVVAAATSSIPEAGGDVALYFEPRDADELAERLRQ
                     ALDDPAIRRNARAAGPAHAARFSWARSGAEMAAVYQRAAAEPARSMV"
     gene            3566..4996
                     /locus_tag="PLC06_07705"
     CDS             3566..4996
                     /locus_tag="PLC06_07705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRO90366.1"
                     /translation="MMRSRVIHWITIVTDLLLINLAFWTAYLARYVWQWLRPVLYLEP
                     YRDYLGQQLVLTILLAIAFRYTGVWRRRRGELWLDEVARIITATAAGIMLMMAVTFFL
                     QPSPFSRLLIFWALLFIVLFLSVSRLLRRLTLSLLYRRGIGVDRVLVVGVGETGRSVI
                     RTLLARPDLGFNMVGYLDEETHENNGHIGRVPRLGGFGDLDRLLREYAPLHTVFIALP
                     GTMQPQLLNMLQICSQHEVRALVVPDLLQLSLNRVEFSNMAGVPVLGMRDMAAFGHIS
                     RSGRVLKRALDLTLILLLSVPTLLVMAVLALIIKLDSPGPIFYTSPRVGREGRVFNMY
                     KFRSMVTDADEQKESLRELNEADGPLFKIKNDPRQTRTGRLIRRLSLDELPQVFNVLL
                     GQMSLVGPRPPLPEEVALYKPWHHQRLAVVGGMTGLWQVSGRSDLSFDELCLLDIYYI
                     ENWTLGSDVRILLQTIPHLVSRKGAY"
     gene            complement(5024..5443)
                     /locus_tag="PLC06_07710"
     CDS             complement(5024..5443)
                     /locus_tag="PLC06_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90367.1"
                     /translation="MARQAIFDGLIFDEYENPVTMAVVGGEAQYVIDDDGFHRHVDAE
                     YVDRQILAFFIEQLSRNKDMAVTQAMEMIGQDDLLTKAAIDASLRNLTADQIIDQGVP
                     AQARDMLGMFGFRVVINVHGDVVGMDQPAAPEEGGDE"
     gene            5784..6845
                     /locus_tag="PLC06_07715"
     CDS             5784..6845
                     /locus_tag="PLC06_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRO90368.1"
                     /translation="MFRPLDWLLGFFSLDIGIDLGTANTLVAIRDKGIVINEPSWVAI
                     NKRTRRPLAIGAEAREMVGRTPGDIVAIRPLRDGVISEFEITEAMLKYFIDKAHEQML
                     VPFPRPRVVVGVPSGVTEVEKRAVYDATISAGAREAYLIEEPMAAAIGAGLPIIESRG
                     SMIVDIGGGTTEVAVFAMGGIVVSRSIRVAGDEMDEDIIGYIRNKYNLLIGERMAERA
                     KMAIGSAFPLPEEKTMTIRGRNLITGLPQVVEISSIELREAMSPSINIIVDTVLDALD
                     ETPPELVSDLIETGICLAGGGAMIQGLADRLEDAVKMRVWVSEDAMTCVARGAGRVLE
                     DLDNYERVLVGLHRGSTRH"
     gene            6888..7742
                     /gene="mreC"
                     /locus_tag="PLC06_07720"
     CDS             6888..7742
                     /gene="mreC"
                     /locus_tag="PLC06_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRO90369.1"
                     /translation="MNLGRTQQRIRWITLAILVGSAILLTILDSTGALGGVMGFVRDP
                     LTYISGWTSARTETVAEVVAGPRNLNTALEEIARLQAENDTLRKENEQLLEAAGEAQI
                     LRELFNRAAETPEYRRITADVVGQDTNPILQSMLINKGFDDGVRVGMPVEAARGLVGQ
                     VYRVTNNAAQVALLTETASAIPVRFGSTRATGMLRGAGRGALPTIDWIDLQYDVQVGE
                     LVTTSGLGGKFPENLVIGRVVDVERNEAELFQRAIVQPAVDFASVEIVFVITEFNTIN
                     TEIFSEEP"
     gene            7747..8247
                     /gene="mreD"
                     /locus_tag="PLC06_07725"
     CDS             7747..8247
                     /gene="mreD"
                     /locus_tag="PLC06_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRO90370.1"
                     /translation="MPNHILVAIPLMAALAVLHTSVLAQLPVLGVAPQLLFLVALAWG
                     IVRGMEEGLAWAFIAGIFTDLFSLAPLGISALAFMAGVAGPLMLRRALPPNRLLVAMF
                     LAGLGTVIYLAVYAVGLRVFGFGIGLGGLTELLPLIGFHAILILPIYLIVDSILRTFR
                     PRRVEF"
     gene            8325..>9436
                     /locus_tag="PLC06_07730"
     CDS             8325..>9436
                     /locus_tag="PLC06_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90371.1"
                     /translation="MSSIGWKPPEERDPEVQQIPGIGGRLLFMRVVIVIVLSLLVYRV
                     YWLQATKGPDLAIRAEQNQFAELTTEPPRGVILDRRGLPLAINKPSFNVTITPAFLPS
                     DEGERQGVFERLALLTGVPMTNTVEQEALIAGANPELVSTYARLAEIYGASVEDTLDE
                     AGIVPRLPNSIEAIVEEHSFAPYIPAVITTGVPISEAYRIEQESIFLPGVRILPQALR
                     EYPTGEFTAHLIGYMGPVPNQNWIDLLGYERDDRVGWAGLESSMEMELAGIKGRRTIE
                     QDWTGREVRQVGEAIAPQAGLNLHLTLDSHLQMITYDILGQFLERNSQTARIDEITGE
                     RTFPEVEQAAVVVLNVKTGEVLAMVNLPTFDNNRFQT"
BASE COUNT         1741 a         2979 c         2883 g         1833 t
ORIGIN      
        1 gttcagcgat ctgttcaagg tgcgggccga cttcagcgac acgatgcccc gcgacgcggc
       61 caacgagatg ctctatgccg ccttcatcgc ttctcgctgc caggaagaga aacttcgccc
      121 cttcggtcgc gatgccgtgg cgataattat cgaagagggc gcgcgactgg ccgaacacca
      181 gcgcaaattg agcacgcgct ttggagccat cgccgacctg gcgcgcgaat ccagctactg
      241 ggccggtatc ggcggccatg aggtcgtgac ggccgacgac gtgcgtgagg cgctcaaaga
      301 gcgcatccat cgcgccagcc aggacgccga acaggtgcgt gaggatattc tggaagggac
      361 gatcttcatc gctacccagg gcgaagccgt cgggcaggtc aacagcctgt cggtgcgtga
      421 agtgggtgat ttcatctatg gccatcccgg ccgaatcagc gcgcgcacgt tcatgggcga
      481 caacggcctg ctccatattg agcgcgagac cgacatggac ggccccattc atgagaaggg
      541 cgtgctgatc ctgaacggct acctcggcgg cacctatgcc cagcaccagc cgctatcgct
      601 gaatgccagc ctgaccttcg agcaaaccta tggcggcatc gacggcgaca gtgcctcatc
      661 gaccgagcta tacgcgctgc tctccagcct cagcggctat ccgatccggc aaggcatcag
      721 cgtcaccggc tcggtcaacc agcgtggcga gatccagccc attggctcgg tcaacgaaaa
      781 aatcgagggg ttcttcgata tctgccacgc gcgcggcctg accggcgatc agggtgtcat
      841 catcccggcc accaacgtcg tgaatctgat gctgcgcgag gatgttgtcg cggccgtttg
      901 cgccggtcaa ttccatgtct ggccgatcac gtccatcaac gagggcatcg aactgctgat
      961 ggggcgaccg gccggtgagg cggatgaggc gggcaactat cctgagggca ccgtccacca
     1021 cgccgtcaag aaacggctga tggagctggc gatggaactc aagtcgttcg gcgacgatcg
     1081 cggtcacgac cagagcgagg acgacgaaga ctagatcatt gctgcgaaag gtgatgcgcc
     1141 aggcgcaaga tggcgatgac gctcttggcg tcctcgaggc ggccgtcaat agccatcgcc
     1201 acaagctctt ccagcggcca cgtctcgacg gtgatctcct cgtcgatatc catgggcagc
     1261 ggatcggccg tcagcccggt cgccacgaag agcgccattc gctcgttgga catccccggc
     1321 gcggcccaca cctcgcccag cgagatcatt tccgccgccc gatagcccgt ctcctcgcgc
     1381 agctcgcgct gcgcgcactg cagccacggc tcatccccct ctcgggttcc ggcgggcagc
     1441 tcgaagagca gggtgcccag agtggggcga tattgacgca gcatcagcag cgccggagcg
     1501 ccgtcagcgg tggtcaccag cggggccagc acgaccgcgc cgggatgctc gaccatcgcg
     1561 cgctcgtagt ccgcgccgtc gtccatgcga acggtattga cgcgaacgcg ccatttaccg
     1621 tcccaaatga tgcgagattc gagaatctcc attagggctt ggggggaatc ctgatctgct
     1681 gcccgacttc gagccgatcg gggttggtaa tcccgttata ttgggccaat tcctggaagg
     1741 tgaatccata gcgcaggccg atgcgataga gattgtcgcc gggctgcacg gtatggatga
     1801 tctcgccgga ggaagaggtt tccgcagtcg gttcggttgg cgcggtttcc tgtgttgtct
     1861 ccccgccggg ttgtgcggcc ggatcagggg ttggcgtcgc gtcggtcggc agcggctggt
     1921 tggggtcggc cgtcatgagc ggcgcgggcg tggggctggc ccccaccggc ggcaggacga
     1981 tggtccccgg ggccaatgtc cccggcgcgg gcgcggtggc cagggcggcg gccgtggatg
     2041 tggccaggaa gttctggctc tcgggcgcgg gaatttcgca accggttaac ccaaacagca
     2101 cgagcattga ggtcaacaac aataagagtg tactgcgttt catgcgcgaa cttctccagt
     2161 gttcaaaccg gcgcgcccat ggtgtgcggc gggtgaacta tcgcggcatg gcctcatcga
     2221 ttatccatgc cgatgacgaa ctgcaatgcc gttcaagaga cggcatgtaa acataatgta
     2281 gtctatcata cagccgaatg aagggcaata tgtcgaataa gccgatgatc gtataattac
     2341 gaggatgttt gcgacaaatg tcaccagaat ttcggggcca acttcggagc catagcgtgg
     2401 cttttccttt gtcacagata cccatgacgc gcgcgcatat cgggattaat gctcacttgc
     2461 ttgccacagc cacaggctac cggcgcgcgg gcatccatca ctacatccat cagatcgtgc
     2521 gccatctgcc gcccacgacc gtgacgcgct acacggtcta tacccgcctc acggatgggt
     2581 gggagcatcg atccgatatg ggcatggtcg gaacgcgtct gccgacggaa aaccgcgccg
     2641 cgcgcatcgt ctgggagcag gccgtgtggc cgttccaggc ccggcgcgac gagttgacgc
     2701 tgatgcacag catggccttc gccatgcccc gcctcgcccc ttgccccgtc gtcgtcacca
     2761 tctacgatct gagttttgtc gagaatcccg aagcgtttcc ggcggcccag cgccgctatc
     2821 tgcagcacga gaccgcctac tcctgcgccc atgccgcacg gctcgtggcc atctccgaat
     2881 caggccgccg cgatatccat cgccacttcg acgtgccgtt ggagcggatc gatgtcgtga
     2941 cgcccggcgt cgatgatcgt tatcgcccgc tgcccgccga tcgggttgcc gccttccggc
     3001 gggaaaaggg gctgcccgaa atcttcatcc tccatgtcgg cacactccag ccgcgcaaga
     3061 atatccccac cctgctggat gctctggccc gcctcgagcg gcccgacgtg gcgctggtgc
     3121 tggtcggcgg ccgcggctgg atttatgagg agatcttcgc ccgcgtgcaa gcgttggggc
     3181 tggccgatcg cgttcgcttc gccggctatg tgggcgacga ggagctgccg ctgtggtaca
     3241 acgccgcagc cgtgctggcc ttcccctcgt tctatgaggg cttcggcatg cccgtcgccg
     3301 acgcgctggc ttgcggcacg ccggtggttg ccgcggccac gtcctccatc cctgaggcgg
     3361 gcggcgatgt ggcgctctat tttgagccgc gcgacgccga tgaattggcc gaacgactgc
     3421 gacaagcgct cgacgatccg gcgatacgtc gaaacgcccg tgcggcaggc ccggctcatg
     3481 cggctcgctt ctcctgggcg cgttcgggcg cggaaatggc ggccgtctat caacgcgcgg
     3541 cggccgaacc ggcgaggagc atggtatgat gcgatcccgg gttatccatt ggataacgat
     3601 cgtcaccgac ctgctgctga tcaaccttgc cttctggacg gcttacctgg cccgctacgt
     3661 ctggcagtgg ttgcggccgg tcctgtatct ggagccgtac cgcgactacc tgggccagca
     3721 gcttgtgctg acgatcctgc tggcaattgc gtttcgctat acgggcgtgt ggcgccggcg
     3781 gcgcggcgaa ttgtggctgg acgaagtggc ccgcattatc accgccacgg ccgcgggcat
     3841 tatgctcatg atggccgtca ccttctttct gcagccgtct cctttctccc gcctgctcat
     3901 tttctgggcc ttgctgttca tcgtcctgtt cctcagcgtg tcccgcctgt tgcgccgcct
     3961 caccctgtcg ctgctctacc ggcgcggcat tggcgtggat cgcgtcctgg tggtcggcgt
     4021 cggcgagacg gggcgcagcg tcattcgcac actcctggcc cggcccgatc tgggcttcaa
     4081 catggtcggc tatctggacg aggaaactca cgaaaataac ggccatatcg gtcgcgtccc
     4141 ccgtctgggc ggcttcggtg atctggatcg gctgctgcgc gaatacgccc cgctccacac
     4201 cgtcttcatc gccttgcccg gcacgatgca accccaactc ctcaacatgc tgcaaatatg
     4261 cagccaacac gaggtgcggg cgctggtcgt gcccgatctg ctgcaattga gtctcaatcg
     4321 cgtggagttt tccaatatgg ccggggtgcc cgtgctgggc atgcgcgaca tggccgcctt
     4381 tggtcacatc agccggtcgg gacgggtctt aaaacgggcg ctcgatctga ccctcatcct
     4441 cctgctgtcc gtgccgacat tgctggtcat ggccgtgctg gctctgatta tcaagctgga
     4501 ctcccccggg ccgatcttct atacctcgcc tcgcgtcggc cgcgaggggc gcgtgttcaa
     4561 catgtacaaa ttccggtcga tggtgaccga cgccgacgaa caaaaagagt cgttgcgcga
     4621 attaaacgaa gccgacggcc cgctgttcaa gatcaagaac gacccgcgcc aaacccgcac
     4681 cgggcgcctc atccgccggc tcagcctcga cgaactgccg caggtcttta atgtattgct
     4741 gggtcaaatg agcctggtag gcccgcggcc gcccctgccc gaagaagtgg cgctctataa
     4801 gccgtggcat catcagcggt tggccgtggt cggcggcatg accggcctgt ggcaggtcag
     4861 cggccggtcg gacctgtcgt tcgacgagct ttgcctgctc gatatctatt atattgagaa
     4921 ctggacgttg gggtcggacg tgaggatact gctccagacc atcccgcacc tggtgtcgcg
     4981 caagggggcc tattagaaaa ggtcgccgag gttccgacga gccttattcg tcaccgcctt
     5041 cctcgggcgc ggccggctga tccataccca ccacgtcgcc gtggacattg ataaccaccc
     5101 gaaagccgaa catccccagc atgtcacggg cctgggccgg gacgccctga tcgatgatct
     5161 gatcggccgt cagattgcgc agcgaagcgt cgatggccgc cttggtcagc aagtcatcct
     5221 ggccgatcat ctccatcgcc tgcgtcacgg ccatatcctt gttccgggac agctgctcaa
     5281 tgaagaaggc cagaatttga cggtcaacat actcggcatc gacatgacgg tggaagccgt
     5341 cgtcatcaat cacatactgg gcctcaccgc cgacgacggc catcgtcacg gggttttcgt
     5401 attcgtcaaa aataagaccg tcaaatattg cttgccgcgc catgaacctg ttactcctgt
     5461 gggacggatt cggctcgaag ccatccatga tattccaaac ccgtaaatct tgattataag
     5521 agattatatc gaacctgttg gcgaaatcgg gatgactcca tcatactctc acccgcagac
     5581 gtcgttaaac caaatatgag cgcaaatacc agctatgtga ccgtcaatag acctatgcta
     5641 taatgccaag gttaacacga caggcatttt cgcctgaatg tgcgtcgatc cgtatcggtg
     5701 tccctggcga taaccagagt ggcgacttca atcacggcgg cgaacaatct gtcaatgaat
     5761 cttggaaagg ataactagtc accttgttcc gaccactaga ctggctgctg ggatttttct
     5821 ctctggatat cggcatcgac ctgggcacgg ccaataccct ggttgccata cgcgataagg
     5881 gcatcgtcat caacgagccg tcgtgggtgg cgatcaacaa gcgcacgcgc cggccgctgg
     5941 ccattggcgc ggaagcccgc gagatggtcg gccgcacgcc gggcgacatc gttgccatcc
     6001 gccccctgcg cgacggcgtg atctccgaat tcgagatcac cgaggcgatg ctcaaatatt
     6061 tcatcgacaa ggcccacgag cagatgctgg tgcccttccc gcggccgcgc gtcgtggtgg
     6121 gcgtccccag cggcgtcacc gaagtcgaga agcgcgccgt ctatgacgcc accatctcgg
     6181 ccggcgcgcg cgaggcctac ctgatcgaag agccgatggc ggcggcgatc ggcgcgggcc
     6241 tgcccatcat tgaatcacgc ggcagtatga tcgtcgatat cggtggcggc acgaccgagg
     6301 tggccgtgtt cgccatgggc ggcatcgtcg tcagccgctc catccgcgtg gccggcgacg
     6361 agatggacga agacatcatc ggctacatcc gtaacaagta caacttgctc atcggcgagc
     6421 gtatggccga gcgtgccaag atggccattg gctcggcctt tccgctgccc gaggaaaaga
     6481 cgatgaccat ccgcggccgc aaccttatca ccggcttgcc gcaagtcgtc gagatcagca
     6541 gcatcgagct gcgtgaggcc atgtcaccgt cgatcaatat catcgtcgat accgttctgg
     6601 acgccctcga cgaaacgccg ccggagctgg tctccgacct gatcgagacc ggaatctgcc
     6661 tggcgggcgg cggcgcgatg attcaggggc ttgccgaccg tctggaagac gccgtgaaaa
     6721 tgcgcgtctg ggtgtctgaa gatgccatga cctgtgtcgc tcgtggtgcc ggtcgtgtgc
     6781 tggaagattt ggacaactac gagcgcgttc tcgtcggcct gcaccgaggc agcacgcgcc
     6841 attagcgtag cctgttccct cgcgaagccg gggcttaccg atcgcctatg aatctggggc
     6901 gaacacaaca acgcatccgc tggatcacac tggcgatcct cgtcggctcg gccatcctcc
     6961 tgaccatcct cgattcaacg ggcgcgttgg gcggtgtaat gggcttcgtg cgcgatcctc
     7021 tgacctatat cagcggttgg acttcggctc gcactgaaac cgtggccgag gtcgtcgccg
     7081 ggccgcgtaa cctgaacacg gcgctggaag agatcgcccg gctgcaggct gagaacgaca
     7141 cactccgcaa ggaaaatgag caattgctcg aagccgccgg cgaggctcaa atcctgcgcg
     7201 aactgttcaa ccgcgccgcc gaaacgcctg agtaccggcg catcaccgcc gatgtcgtcg
     7261 gccaggacac caaccccatc ctgcagagca tgcttatcaa caagggcttc gatgacggcg
     7321 tgcgcgtcgg catgcccgtc gaagcggcac gcggcctggt gggccaggtc taccgcgtca
     7381 ccaataatgc cgcccaggta gctttgctga ccgaaacagc cagcgccatc cccgtgcggt
     7441 tcggctccac gcgggccacc ggcatgttgc gcggcgcggg gcgaggcgcg ctgccgacca
     7501 tcgactggat cgacctgcaa tatgacgtcc aggtcggcga actggtgacg acatcagggt
     7561 tgggcggcaa attcccggag aacctggtga tcggccgcgt ggtcgacgtg gagcgcaacg
     7621 aggctgagtt gttccagcga gccatcgttc agccggccgt cgacttcgcc tcggtggaga
     7681 tcgtcttcgt catcaccgag ttcaacacca tcaacaccga gatattcagc gaggagcctt
     7741 agctctatgc ctaatcacat cctcgtcgcc attcccctga tggccgcgtt ggcggtcttg
     7801 cacacgtcgg tgttggccca gcttccggtc ttgggagtcg cgccgcagct tctgtttctg
     7861 gtggctctgg cctggggcat cgtccgcggg atggaagaag gcctggcctg ggctttcatc
     7921 gccgggatat tcaccgatct gttttcgctc gcgccgttgg gcatttcggc actggcgttc
     7981 atggccgggg tcgccgggcc gctgatgctg cgccgcgcat tgccccccaa ccgtctgctg
     8041 gtggccatgt tcctggccgg gttgggaaca gtcatctatc tggctgttta tgccgtcggc
     8101 ctgagggtgt tcggcttcgg gattggcctg ggagggctga ccgagctatt gccgttgatc
     8161 ggctttcatg ccatcctgat cctgcccatc tacctgattg tcgattccat cctccgcact
     8221 tttcgcccgc gtcgggtcga attttgatcg cataaaacat agcgccgtct tagaatcaac
     8281 ggtaaacccg tgaattcagg cccgaattgg gcataatagg ctggatgtcg agcatcggat
     8341 ggaagccacc agaggagcga gacccggagg tccaacaaat ccccggcatt ggcggccgcc
     8401 tgctgttcat gcgtgtcgtc atcgtcatcg ttctaagcct cctcgtctat cgagtctact
     8461 ggctgcaggc gaccaaagga ccggatttgg ctattcgcgc cgaacaaaac cagttcgccg
     8521 agctgacgac cgagccgcca cgcggcgtca tcctcgatcg ccgagggctg ccgctggcca
     8581 tcaacaagcc gagcttcaac gttaccatca cccccgcctt cctgcccagt gacgagggcg
     8641 agcgccaggg cgtgttcgag cgcctggcgc tcctgaccgg cgtcccgatg accaacacgg
     8701 tcgagcagga agcgctgatc gccggcgcca accccgaact tgtcagcacc tacgcgcgcc
     8761 tggccgagat ctatggcgcg tcggttgaag ataccctgga tgaggccggc atcgtgcccc
     8821 gcttgcccaa cagcatcgag gctatcgtcg aggaacacag ttttgcgccc tacattccgg
     8881 cggtcatcac gaccggcgtg cccatctccg aagcctatcg catcgaacag gagagcatct
     8941 ttctgccggg cgtgcgcatc ctgccccagg ctctgcgcga atatcccacg ggcgagttta
     9001 cggctcacct gattggctat atgggtccgg tgccgaacca gaactggatc gacctgttgg
     9061 gctatgagcg cgacgaccgg gtcggctggg ccgggctgga atcgtcaatg gagatggaac
     9121 tggccggcat caaaggccgt cgcaccatcg aacaagactg gaccggccgc gaagtgcgcc
     9181 aggtcggcga ggcgattgcg ccgcaagcgg gcctgaactt gcatctgacg ctcgattccc
     9241 acctgcagat gatcacgtat gacatcctgg gccagttcct ggagcgaaac agtcaaacgg
     9301 cccgcattga cgaaatcacc ggcgagcgca cctttccgga ggtcgaacag gcggcggtgg
     9361 tcgttctcaa tgtaaagacc ggcgaagtgt tggccatggt caacctgccg acattcgaca
     9421 acaaccggtt ccagac
//
LOCUS       DAOMQG010000107         9392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3151_length_9392_cov_9.461604, whole genome shotgun sequence.
ACCESSION   DAOMQG010000107 DAOMQG010000000
VERSION     DAOMQG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_9392_cov_9.461604"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..711
                     /locus_tag="PLC06_07735"
     CDS             64..711
                     /locus_tag="PLC06_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90372.1"
                     /translation="MSRFGYFRGGGGGDLGYADVSGLDSSEAGALYQAAIIAALGLDV
                     GERLGAVAAGGAAFETEPGSTPISFATLAEFLTGAAANLTLSLDVARYMGRPKAVTYS
                     TTVTLRLDGVREADDEAADHDSDSLISTIACTGNCAAGFDGIDPNLAWQSGLVVFVNT
                     GGGARTVSLATANGVTVTGLSEFPLGTVAGDMLAVGYTVISATRVHIDSIRAIAA"
     gene            708..1475
                     /locus_tag="PLC06_07740"
     CDS             708..1475
                     /locus_tag="PLC06_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90373.1"
                     /translation="MSLRSLAFIGPEPLIVEDEPPGGWVPTLLANAGTGYLTYEGSFG
                     TWAPDASSLDLAFLVGGLDGNPNANCYIFQNHGDRVRVRFMPDGKLHARLQDASATPV
                     DLVDWTSSAGVDAAGVWLFHVAAALSGTPSFTVYKIEVQETSPGVWETVGSWAAITGT
                     FATGPVAGTIDIARNFSGPDLGIFATVGGASIPDCDFGYLWLSSASPLANSAFADGGL
                     LIDPALAGTPAILIKAPNVTDGQGTANLTFVGNGAWV"
     gene            1472..5662
                     /locus_tag="PLC06_07745"
     CDS             1472..5662
                     /locus_tag="PLC06_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90374.1"
                     /translation="MNRPFTYGGSVAVTREGIMLLEPVAGAPSPNLGVTVTLTAPDAS
                     GGRYVEITRPGALAADVTKVRITSFGFVGGEKKSCQRTLPVEILTAMKAGGGTTRLPG
                     GADENWLGALAGTDPTQIVMTLLNASGWGVVRDLWAYTPPAPTEFEPFVVTANPILTG
                     PLTGGDPWSYTPLAWTGEESVEITHVYRMAEAGGANPAATTGEITATAPNISGSLTHV
                     RIEATASGEGVANPETVYASDWAEIEAPGVETPVTVATPPLMASNRLLVDGITDANFA
                     PRDTLTAGGASATVVKYVPDTSELFLGPITGGPIADNATLSSTSGGAATANGASQAWG
                     FTAGEPFYLLPPLFNGTPTDVTFLQYVREDSDDATPEEVEFTGVCPAHYGTKQYQPLW
                     RYSGYGIETFAEVSLDWLNIGIPTLAPLAVTDWKIIVAAEPGGGDRVTEVYVRDNLNI
                     SAAMATTSTQAFIEANQFPAGFEPTTLGATITEGGIVYRRCAIIPWVHNGIDYSVFGA
                     GATGNEAARRGNFGLVVQIGGAWSPLGDRKTVPVATGTPSAAGSFQRIVPRSQEWVED
                     GEGGGTHMQFPRGFWGQGDFFVMAHDVTWPAVTKDFGDNWESDDCLGLFCQQTTTGIC
                     VDLPYVHGQFGPLYLEGINDSYEDPWGGTYRKNVDTNQWEQTKQLSQVCGSNAGNTRR
                     NMRYICKVAGSGSGPDTRTLYQIHAPATSPSNFSTIQIQRSTQGGAPGSWSNFGSSMA
                     TGTHGMPLEIWATATHLFLNTLTKVRCRTVGTDTWADATGLPGGEKLHLEVHGSTCYV
                     FVKGQGLYTATVGGGAALAFTRLKQFNGLTFSVSPADPQRIVLVAANRTVTPIGTHNG
                     GASWFNIASLPYPGQPEDFEHRLNNNPAWAYFHDTDPDLVLAMRYQHMGKSTDGGRTF
                     VWASRGLDYSEVRWIGFHPTDPLRFFLTMTDKLTVAMDGGIALDDKLDADAKATIEAY
                     LGGDPLSAYTAGGAMILSRGAHSAYISSVGKNVGKKTPVVSNRGYTVTESDRPSTGNG
                     AISVSITPDLPCGEYDLTCITAAANGGTFKLVGPVNVDMGNFTVGTQRTATHPRGGSI
                     TITVSDGSVDFKAGANPKQFTIRVEPIKTNTILNPGTKGSGYYGGTNPGVPHRGITGR
                     SVFELATDGSSSIVRTIPYEFMGYMGTDGTVVAASGNSTLMRGNDTGGSFTNWANAGG
                     NFNPMGRQVVIGSRHSNQRAYCGLSNGRVKRFDSGTPQEIFSFDAFCTQHGISGDWPG
                     TATVNGRMVPPCTGLWESAFDPNMLWACFYAVGMPMNLFRTRDALSANPTWYNCTAEG
                     LVGWGPIAADIKGRGLMGPLQAMTGSPVTDEPVVFSQHGTVMIKPPPDHIATYSLPSV
                     IDYIRSLPGGNYYDTARL"
     gene            5811..8219
                     /locus_tag="PLC06_07750"
     CDS             5811..8219
                     /locus_tag="PLC06_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90375.1"
                     /translation="MPYPPLADIETSYTAFQQGQGDNSFPGQQIDTDLANLKEAVDTL
                     NEFVRGVTRSDGEVGNQTIGHDQLKPELSIGLEPPVTWATATDYEAGQTVFTGLGYYR
                     ATADHTSSVSFAADLATGLWELLIDFQSSLAEELPAVVEPIVEGMAPGIVDDALAQVP
                     AIVTPMIEAAAPGIVAQAAALNLRRYVDVFDYVPANDMLDCWDGVASALVDAADRELV
                     FTAGAVGGTRTYRFTDRTKGRLYLPKKARLIWEPGAVMDFSSWGRSGTVSPYLYCQGD
                     TAAATALASDGAEGATTLTLASGLGGDYARGDQGILVSDALFTTEDSGLGTKGEWVKI
                     ASVAGDVLTLVGRLRDTYLTTDNARLHRMTAPAELTLENVQILGAGRRTADELGDRGI
                     QIINGVNCQVKGGWVKYADFNGVSFTNILGGRIDGVYVENEPKGGVNTQNQYPFVLVN
                     ACDGTVVSSCFADGGKEGFCLSVTGGIQAVTRNATFDTCLARNMWRSGFCSHDAHTAW
                     HVLNCTAEDCEQGLDIRIQDAVVDNFTARRMGRHGGTLDCAIHIGSGAGQVRITNLKV
                     IDALRGVWMPSAIVHETIPGNISIDGAVMRGIRGHGVLLDHRTDVNPAMTGALGTVNI
                     SNVDMVAVTSTTVPQGINLHGKWSNPTLRDIVLQGGNGGAAVVMRATSNGGGTGGSIN
                     PDIEVTYGSGFVSSVVQHATGVTKVREKLIGGYAPGEVLRASKTHDWGDIAGGAVLGE
                     PVTCAGATIDDYAKASMSIAHVGIRLDAWVTATDTVTVYATNQTGGAINLASGTLYVE
                     VTKR"
     gene            8235..9212
                     /locus_tag="PLC06_07755"
     CDS             8235..9212
                     /locus_tag="PLC06_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90376.1"
                     /translation="MADPTPYVVSYSFSGFQATSPFAPLPAPRVDDEFANIANSIGTI
                     NAAVRDVRREDGELRNGIVTLDSLAVEVGTRLGVAYANAYDVAVAEGFVGTVDEWLAS
                     LAANVTVGSVTTGAAGSDAEVVVTGTAPDKVLSFTLPRGAAGPSGDGSGDMLVSVYDP
                     NAKEADAFDMANMVEGANAKVLTASERAAIATIASKLGASDVASVAQIRANTAGKVID
                     TTGAWGSLAFVTLSGSPTLAIDFATFINGEVTTSSDRTLGQPSNVQVGQSGCIKITAS
                     GATRSISKHSYYKSATPFPIDIAAGATAYLFYFCASATEVLLNVVAEAT"
     gene            9213..>9392
                     /locus_tag="PLC06_07760"
     CDS             9213..>9392
                     /locus_tag="PLC06_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90377.1"
                     /translation="MTPFVAPAGMMADSRYTVAQVDIRSTLSSPAPTFAGVKLGAEAA
                     TRGIVVFAWMADVTTP"
BASE COUNT         1577 a         3380 c         3035 g         1400 t
ORIGIN      
        1 tctatgtgcg gtcgcgctcc ggcgcgatca cgctcgtctc cacgaccgac gcgggtgcgt
       61 gacgtgagtc gcttcgggta tttcaggggc ggaggcgggg gcgatctcgg ctacgcggac
      121 gtcagcggcc tcgacagcag cgaggccggc gcgctctacc aggcggcgat catcgccgcg
      181 ctcgggctcg atgtgggcga gcgcctcggt gccgtcgcgg cgggcggcgc ggcgttcgag
      241 acggagccgg gcagcacccc gatctccttc gccacgttgg ccgagttcct gaccggcgcg
      301 gctgccaacc tcaccctgtc gctcgacgtg gcccgctaca tgggccgccc caaggcggtg
      361 acctactcga cgacggtgac gcttcgcctc gacggggtgc gtgaggccga cgacgaggcg
      421 gcagatcacg acagcgacag cctcatctcg accatcgcct gcaccgggaa ctgcgcggcg
      481 ggtttcgacg gcatcgaccc gaacctcgcc tggcagtcgg ggctcgtcgt cttcgtgaac
      541 accggcggcg gcgcccggac ggtcagcctc gcgacggcca acggggtcac cgtcaccggg
      601 ctctccgagt tcccgctcgg caccgtcgcc ggcgacatgc tcgccgtggg ctacaccgtc
      661 atcagcgcga cccgcgtcca catcgacagc atccgggcga ttgcggcatg agcttgagat
      721 cgctcgcctt catcggcccc gagcccctca tcgtcgagga cgagccgccg gggggctggg
      781 tgcccacgct cctcgccaac gcaggcaccg gctatctgac atacgaaggc agcttcggaa
      841 cctgggcgcc cgacgcttcc agcctcgacc tcgcctttct cgtcggcggc ctcgacggca
      901 acccgaacgc caactgctac atcttccaga accacggcga ccgcgtccgc gtccgcttca
      961 tgcccgacgg caagcttcac gcgcgactcc aggacgcgtc cgcaaccccg gtggacctcg
     1021 tcgactggac gagctcggcc ggggtggacg ccgccggggt ttggctcttc cacgtcgccg
     1081 cggcgctgtc aggcacgccc tccttcacgg tctacaagat cgaggtgcag gagacttctc
     1141 ccggggtgtg ggagacggtc gggtcatggg ccgccatcac cggcacgttc gccaccggcc
     1201 ccgtcgcggg gacgatcgac atcgcgcgca acttcagcgg ccccgatctg ggcatcttcg
     1261 ccacagtcgg cggggcgagt atcccggact gcgacttcgg ctacctgtgg ctttcgtctg
     1321 cgtcgccgct tgcgaactcc gccttcgctg acggcggctt actcatcgac cccgccctcg
     1381 ccggcacccc cgccatcctc atcaaggcgc ccaacgtcac cgacggccaa ggcaccgcca
     1441 acctcacctt cgttggcaac ggagcctggg tatgaaccgg cctttcacct atggcggctc
     1501 ggtcgccgtc acccgagagg ggatcatgct gctggagccc gtggcgggcg ccccctcgcc
     1561 caacctcggc gtgacggtca cgctgaccgc ccccgacgca tccggcggcc gctacgtcga
     1621 gatcacgcgc cccggcgctc tcgccgccga cgttaccaag gtgaggatca cgtccttcgg
     1681 gttcgtcggc ggcgagaaga agtcctgcca gcggaccctt ccggtggaaa tcctgaccgc
     1741 gatgaaggcc ggcggcggca cgacccgcct gcccggcggc gccgacgaga actggctcgg
     1801 ggcgctcgcg ggaaccgacc ccacccagat cgtgatgacc ctcctgaacg cctccggctg
     1861 gggcgtcgtg cgcgacctgt gggcctacac gccccccgct cccacggagt tcgagccctt
     1921 cgtcgtcacc gcgaacccga tcctcaccgg gccgctcacc ggcggcgatc cgtggtccta
     1981 caccccgctg gcgtggaccg gcgaggagag tgtcgagatc acccacgtct accggatggc
     2041 ggaggcgggc ggcgcgaacc cggccgccac caccggcgag atcaccgcca ccgcgccgaa
     2101 catcagcggg tcgctgaccc acgtccgcat cgaggccacg gcctccggcg agggcgtagc
     2161 gaaccccgag accgtctatg cctcggactg ggcggagatc gaggcccccg gcgtcgagac
     2221 cccggtgacg gttgccaccc cgccgctgat ggcaagcaac cggcttctcg tcgacgggat
     2281 caccgacgcg aacttcgcgc cgcgcgacac gctgacggcc ggcggcgcga gcgccaccgt
     2341 cgtcaagtac gtgccggaca cgagcgagct cttcctcggg ccgatcaccg gcggcccgat
     2401 cgccgacaac gccacgctct cctcgacgag cggcggcgcc gcgacggcca acggggcgag
     2461 ccaggcctgg ggcttcaccg ccggcgagcc gttctatctc ctgccaccac tattcaacgg
     2521 cacgcccacc gacgtgacct tcctgcaata cgtccgcgag gacagcgacg acgccacccc
     2581 cgaggaggtg gagttcaccg gcgtctgccc ggcgcactac gggaccaagc agtatcagcc
     2641 gctctggcgt tattcgggct acggcatcga gaccttcgcc gaggtgtcgc tcgactggct
     2701 caacatcggc atcccgacgc tcgcgccgct cgctgtcacc gactggaaga tcatcgtcgc
     2761 ggcggagccg ggcggcggcg accgcgtcac cgaggtctac gtccgcgaca acctcaacat
     2821 ctccgccgcg atggcgacga cgagcaccca ggcgtttatc gaggcgaacc aattccccgc
     2881 aggcttcgag ccgacgacct tgggggcgac gatcaccgag ggggggatcg tctaccgccg
     2941 ctgcgcgatc atcccgtggg tccacaacgg catcgactac agcgtcttcg gcgccggcgc
     3001 gacggggaac gaggcggcgc gacggggcaa cttcggcctc gtcgtgcaga tcggcggcgc
     3061 ctggtcgccg ctcggcgacc gcaagaccgt cccggtggcg accggcaccc catcggcggc
     3121 cggctcattc cagcggatcg tcccgcgctc gcaggagtgg gtcgaggacg gcgagggcgg
     3181 cggcacgcac atgcagttcc cgcggggttt ctgggggcag ggcgacttct tcgtgatggc
     3241 ccacgacgtg acgtggccgg cggtcaccaa ggacttcggg gacaactggg agtcggacga
     3301 ctgcctcggc ctcttctgcc agcagaccac caccggcatc tgcgtcgacc tcccctacgt
     3361 ccacggccag ttcgggccgc tctacctgga ggggatcaat gactcctacg aggatccgtg
     3421 gggcggcacc taccgcaaga acgtcgacac taaccagtgg gagcagacca agcagctctc
     3481 ccaggtctgc ggctccaacg ccggcaatac tcgccggaac atgcgctata tctgcaaggt
     3541 cgcgggctcg ggctccggcc cggacacccg cacgctctac cagatccacg ccccggcgac
     3601 gagcccgagc aacttctcga ccatccagat ccagcgcagc acgcagggcg gcgccccggg
     3661 ttcgtggtcg aacttcggct cctccatggc gaccggcacc cacggcatgc cgctcgagat
     3721 ctgggcgacc gcgacccacc tcttcctcaa cacgctgacc aaggtgcggt gccgcacggt
     3781 cggcaccgat acatgggcgg acgccaccgg cctgccgggc ggcgagaagc tccacctcga
     3841 ggtgcacggc agcacctgct acgtcttcgt aaaggggcag ggcctttaca cagccaccgt
     3901 cggcggcggc gctgcgctgg cgttcacccg cctcaagcag ttcaacggcc tgacattctc
     3961 ggtctcgccg gccgacccgc agcggatcgt gctggtcgcc gcgaaccgca ccgtcacccc
     4021 gatcggcacc cataacggcg gggcgagctg gttcaacatc gcgagcctgc cctaccccgg
     4081 ccagccggag gacttcgagc accggctgaa caacaatccc gcctgggcat acttccacga
     4141 cacggacccc gatctcgtgc tggcgatgcg ctaccagcac atgggcaaga gcacggacgg
     4201 cggccgaacc ttcgtctggg cttcgcgcgg gctggactac tccgaggtgc gctggatcgg
     4261 cttccacccc accgatcccc tgcgcttctt cttgacgatg accgacaagc tcaccgtcgc
     4321 catggatggc ggtatcgcgc ttgacgacaa gctggatgcc gacgccaagg ccacgatcga
     4381 ggcctacctc ggcggcgatc cactctcagc ctacactgcc ggcggggcga tgatcctctc
     4441 ccgcggcgcc cactccgcct acatctcgag cgtcggcaag aacgtcggca agaagacccc
     4501 ggtagtctcg aaccgcggct acaccgtcac cgagagcgac aggccaagca ccggcaacgg
     4561 ggcgatcagc gtctcgatca ctcccgacct tccctgcggc gagtatgacc tgacctgcat
     4621 caccgccgcg gcgaacggcg gcaccttcaa gctcgtcggc ccggtcaatg tcgacatggg
     4681 caacttcacc gtcggcaccc agcgcaccgc cacgcacccg cgcggcggct cgatcaccat
     4741 cacggttagc gacggctctg tcgacttcaa ggccggcgcg aacccgaagc agtttacgat
     4801 ccgcgtcgag ccgatcaaga ccaacaccat cctcaacccg ggcaccaagg gtagcggcta
     4861 ctacggcggg accaaccccg gcgtcccgca ccgcggcatc accgggcgct cggtcttcga
     4921 gctcgccacc gacggcagca gctcgatcgt ccggacgatc ccctacgagt tcatgggcta
     4981 catgggcacc gatggaacgg tcgtcgccgc cagcggcaat tctacgctga tgcgcggcaa
     5041 cgataccggc ggcagcttca cgaactgggc gaacgccggc ggcaacttca atcccatggg
     5101 gcgccaggtc gtcatcggct cgcgccacag caaccagcgc gcctactgcg gcctctcgaa
     5161 cgggcgcgtg aagcggttcg acagcggcac cccgcaggag atcttcagct tcgacgcctt
     5221 ctgcacccag cacggcatca gcggggactg gccgggcacc gccaccgtca acggccgcat
     5281 ggtgccgcca tgcaccgggc tctgggagag cgcgttcgat ccgaacatgc tctgggcatg
     5341 cttctacgcc gtcgggatgc cgatgaacct gttccgcacc cgagacgcgc tctcggcgaa
     5401 cccgacctgg tacaactgca ccgcggaggg gctcgtcggc tgggggccga tcgccgccga
     5461 catcaagggc cgtggcctca tggggccgct ccaggccatg accgggagcc cggtgaccga
     5521 cgagcccgtc gtgttcagcc agcatggcac ggtcatgatc aagccgcccc cggaccatat
     5581 cgcgacctac tcgctcccct cggtcatcga ctacatccgg tcgctccccg gcggcaacta
     5641 ctacgacacg gcgaggctgt gatcctgacg gcctccggca cctaagccgc aatcccgacc
     5701 acccccgcct gcggggcctg tccctgcccc atcgggaccg gtcagcggct gacgttgcgc
     5761 cacacgcgaa tgtcggatat tgttggccga cgtttcgcgc aggagcagac atgccctacc
     5821 ccccgctggc cgatatcgag acgagctaca ctgcgttcca gcaggggcag ggggacaatt
     5881 cgttcccggg ccagcagatc gacacggacc tcgcgaacct caaggaagca gtagacacgc
     5941 tcaacgaatt tgttcgcggc gtgacgcgat cggacgggga ggtcggcaat cagacgatcg
     6001 ggcacgacca actcaagccg gagctgtcca tcggcctcga gccgcccgtc acctgggcga
     6061 ccgccaccga ctacgaggcc ggccagacgg tgtttaccgg gctcggctac taccgcgcca
     6121 ccgccgacca cacctcgtct gtcagcttcg cggccgacct cgctacaggg ctgtgggagt
     6181 tgctgatcga cttccagtcg tcgctggccg aggagctccc cgccgtcgtc gagccgatcg
     6241 tcgaggggat ggcccccggg atcgtcgacg acgccctggc gcaagtcccc gccatagtca
     6301 cgcctatgat cgaggccgcg gcccccggga tcgtcgcgca ggcggctgcc ctcaatctgc
     6361 ggcgctacgt ggacgtcttc gattacgtcc cggccaacga catgctcgac tgctgggacg
     6421 gcgtggcgtc cgccttggtc gacgcggccg atcgcgagct cgtcttcacc gccggcgctg
     6481 tcggcggcac ccggacctac cgcttcaccg accggaccaa gggccgactc tacctgccga
     6541 agaaggcacg gctgatatgg gagccgggcg cggtcatgga cttctccagc tggggccgga
     6601 gcggcaccgt ctcgccctac ctctactgcc aaggcgacac cgccgcggcg accgccctcg
     6661 ccagcgacgg cgccgagggg gccacgacgc tgaccctcgc gagcgggctg ggaggtgatt
     6721 acgcgcgcgg ggaccagggc atcctcgtct ccgacgcgct gttcacgacg gaggacagcg
     6781 ggctcgggac caagggcgaa tgggtcaaga tcgcgagcgt agccggggac gtgctgaccc
     6841 tcgtgggccg gctgcgcgac acctatctga cgaccgacaa cgcccggctc caccgcatga
     6901 cggcaccggc cgagctcacc ctcgagaacg tccagatcct cggcgcgggc cgccgcaccg
     6961 ccgacgagct cggggatcgc ggaatccaga tcatcaacgg cgtgaactgt caggtcaaag
     7021 gggggtgggt gaagtacgcc gatttcaacg gcgtctcgtt taccaatatt ctcggcggga
     7081 ggatcgacgg cgtttatgtc gagaacgagc cgaagggcgg cgtcaacacg caaaaccagt
     7141 acccttttgt gctggtgaac gcctgcgatg gtacggtggt gtccagttgc ttcgcggatg
     7201 ggggcaagga aggcttctgt ctgtccgtaa caggcggcat ccaggccgtt actcgcaacg
     7261 ccacctttga cacctgcctt gctcggaata tgtggcgctc gggcttctgc agccacgacg
     7321 cgcataccgc atggcatgtg ctgaattgca ccgccgagga ctgcgagcag ggcctcgaca
     7381 tccgtattca ggacgcggtg gtggacaact tcaccgcccg ccgcatgggc cggcacggcg
     7441 gcaccctcga ctgtgctatc catatcggct ccggcgccgg gcaggttcgc atcacgaact
     7501 tgaaggtgat cgacgcgctc cggggggtct ggatgccgtc agcgatcgtg catgagacga
     7561 tcccgggcaa tatctcgatc gacggcgcgg tcatgcgggg cattcgcggc cacggggttc
     7621 tgctcgacca ccgcaccgac gtgaaccccg ccatgacggg ggcgctcggc accgtcaaca
     7681 tctcgaacgt cgatatggtc gccgtcacca gcacgacggt cccgcagggc atcaacttgc
     7741 acggcaaatg gtcgaacccg acgctgcgcg acatcgtgct tcaaggcggc aacggcggcg
     7801 cggcggtagt aatgcgcgcc accagcaacg gcggcggcac cggcggttcg atcaaccccg
     7861 acatcgaagt aacctacggg tcggggttcg tgtcttcggt ggtccagcac gctacgggcg
     7921 tgacgaaggt acgggaaaag ctgatcggcg gctacgcccc gggcgaggtg ctccgcgcga
     7981 gcaagacgca cgattggggc gatattgccg gcggggcggt cctcggcgag cccgtgacct
     8041 gcgccggcgc gacgatcgac gactacgcca aggcgtccat gagcatcgcc catgtcggca
     8101 tccggctcga cgcctgggtg actgcgacgg atacggtgac ggtctacgcc acgaaccaga
     8161 ccggcggcgc catcaatctc gcttccggca ctctctacgt cgaggtcacg aagcggtgac
     8221 gaggagcgga aaacatggct gacccgacgc cctacgtcgt ctcctactcg ttttcggggt
     8281 ttcaggcgac gagccccttc gcccccttgc cggcgccgcg ggtcgacgac gagttcgcca
     8341 acatcgccaa ctcgatcggc acgatcaacg cggcggtgcg ggacgtcagg cgcgaggatg
     8401 gcgagctccg aaacggcatc gtcacgctcg actccctggc cgtcgaggtc ggcacgcggc
     8461 tcggcgtcgc ctacgcgaac gcctacgatg tggcggtcgc cgagggcttc gtcggcacgg
     8521 tcgacgagtg gctggcctct ctcgcggcga acgtgacggt gggcagcgtg acgaccggcg
     8581 cagcgggcag cgatgccgag gtagtcgtca cgggcacggc gccggacaag gttttgagct
     8641 tcacgctgcc acgcggcgcc gcagggccgt ccggcgacgg ctcgggcgac atgctcgtgt
     8701 cggtctacga tccgaacgcg aaggaagccg acgcattcga catggcgaac atggtcgagg
     8761 gggccaacgc caaggtgctc accgcgtccg agcgcgccgc gatcgccacg atcgcgtcga
     8821 agcttggcgc gagcgacgtg gcctccgtcg cccaaatccg cgccaacacg gccgggaagg
     8881 tgatcgacac cacgggcgcc tgggggtcgc tggcgttcgt gacgctcagt ggctccccca
     8941 cgctcgccat cgacttcgcg acgttcatca acggcgaagt aaccacatcg tcggaccgga
     9001 cgctcgggca gccgtccaac gtacaggtcg gccagtcagg gtgcatcaag atcacggcat
     9061 cgggcgcgac ccggagcatc agcaagcatt cgtactacaa aagcgcgacc cctttcccta
     9121 tcgacatagc cgcgggcgcg acggcgtatc tgttctactt ctgcgcgtcc gcgaccgagg
     9181 tgctcctcaa cgtcgtggcg gaggcgacct aggtgacccc cttcgtcgcc cccgccggga
     9241 tgatggccga ctcgcgctat acggtggcgc aggtcgacat ccgttccacc ctcagctccc
     9301 cagcccccac gtttgccggc gtgaagctag gcgcggaagc ggcgacgcgc ggcatcgtgg
     9361 tcttcgcgtg gatggcggat gtgacgacgc cc
//
LOCUS       DAOMQG010000108         9266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3195_length_9266_cov_7.274020, whole genome shotgun sequence.
ACCESSION   DAOMQG010000108 DAOMQG010000000
VERSION     DAOMQG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3195_length_9266_cov_7.274020"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1090
                     /locus_tag="PLC06_07765"
     CDS             236..1090
                     /locus_tag="PLC06_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRO90378.1"
                     /translation="MKFNVALAQIAPTLGDVAANLEAHLVAIDEARAAGAALIVFPEL
                     SLTGYALGDRAWDVAIRPTADDPTFLKLLAASAKIDIVAGFVEIDDRHRLYISAAYLA
                     GGGLVHLHRKLYLPTYGLFNESRVYTPGQSLRAFDTRFGRVGMLICEDIWHASSPWLL
                     WQDGADILILISASVEHNQGEALSTAGKVLSMCRAYALLFTDFVVHVNQTGEDDSGRY
                     WGNSVVYGPDASLLIEGPRDEAAVVTGEIDTALLAPARRALPLLRDERADLVARELGR
                     ILMIDAGM"
     gene            1129..3396
                     /locus_tag="PLC06_07770"
     CDS             1129..3396
                     /locus_tag="PLC06_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRO90379.1"
                     /translation="MTQEYIEIHNARENNLKNVSLRVPKRQITIFTGVSGSGKSSIVF
                     DTIATEGQRLLNENFSMFVRNFLPRFGRPDADVIANLSMPVVVDQKRMGGGSHSTVGT
                     ITDISPVLRLLFSRAGQPFVGYSNVFSFNDPQGMCPECNGLGRKLAVDMERFLDTSLS
                     LNEGALLGPGYAVGKWDWSFIVQSGLFDVDKKLDDYSAEELELLLHSPPRKVQTEFGG
                     RTINLTFEGVVAKFTRKYIKQDTANHSERTLKTVAPYITEGPCPACHGARLNQAALGC
                     RINGYNIADLQSMEIAHLIEVIRAIDSPVNVTMIATLTERLQHLIDIGLEYLTLSRPT
                     DTLSGGESQRVKIVKQLSSSLIDVMYIFDEPSIGLHPRDIHLMNELLQKLRDKGNTVL
                     VVEHDPAVIAIADHVVDVGPRAGAAGGEVVYEGKLQGLLQSGTLTGRYMGQTLPIKAQ
                     FRPPKGLLPIRNARANNLQNVSVDVPAGVLTVITGVAGSGKSSLINQTFLKRHPEAIV
                     IDQAAVGANSRSNPATYTGILDDVRAAFARANEVSASLFSFNSEGACENCNGAGVIYT
                     DLGFMDGVKTPCEICEGRRFKDEVLTYKLDGKTITDILNMTAREALDFFDIPKVTVKL
                     RAMVDVGIGYLTLGQPLSTLSGGECQRIKLAGELHKKGSIYVMDEPTTGLHMADISLL
                     LAIMNRLVDAGNTVIVIEHNLDVIRNADWIIDMGPEGGSKGGRVIFEGTPQALLAAEG
                     SLTGRYLNHDHSQIT"
     gene            3552..4985
                     /locus_tag="PLC06_07775"
     CDS             3552..4985
                     /locus_tag="PLC06_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRO90380.1"
                     /translation="MLIQHKFYINGQWVEAHSRQTAEVINPATEEAIASIAMGDETDV
                     DRAVAAARAAFDNYAQWSVAERVALFERIIAVYDRRMAEMAETISQEMGAPLAFATIA
                     QAPSGRDQFKATLEALKSFEWEKEVGRSRVVREPIGVCALITPWNWPQNQVVSKVAPA
                     LAAGCTMVLKPSEITPLDAILLAGILDEAGVPAGVFNLVNGDGAGVGTALARHPDVDM
                     ISFTGSTRAGVLVAQNAASTVKRVTQELGGKAANIILDDARLEKVVAGGVRVCVRNAG
                     QSCNAPTRMLVPMSRMDEAAAVAREAALAIRVGDPTAADTEIGPVASKTQWDKIQNLI
                     RKGIEEGARLECGGPGLPDGLKRGYYVRPTVFSHVDNRMAIAREEIFGPVLVIIGYED
                     EEDAIRIANDTPYGLTGYVQSGDLERARRVARRIRAGSVHLNGAPGGYDVPFGGYKQS
                     GNGREQAGWGLEEYLETKAIIGYYPKS"
     gene            5009..5998
                     /gene="era"
                     /locus_tag="PLC06_07780"
     CDS             5009..5998
                     /gene="era"
                     /locus_tag="PLC06_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRO90381.1"
                     /translation="MNDEQEPDIIEEPNDVHDAHDAAQYETPEGHRSGFVALVGRPNV
                     GKSTLINAFMRQKIAAVTPRPQTTRTRQLGIITEPDYQMIFIDTPGIIRDPRHDLDSF
                     MVQSALETLDDADVVLWLVDMSENPGAADRDIAKLLAEGERKVILALNKIDAIGPTEA
                     LARTEAYVSLLRSAEWLHLSALTGDGVAILLDKLVAALPEGPRYYPAEQITDIYERNI
                     AAELIREQIMLLLRDEVPYGTAVKVRDFKERDNGSTYVSADIFVERDSHKSIVIGKNG
                     AQLKAIGAAAREQIEQLTGGKVFLELWVKVEKDWRRDPNLLRLFGYSNEWTKE"
     gene            6207..6593
                     /locus_tag="PLC06_07785"
     CDS             6207..6593
                     /locus_tag="PLC06_07785"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HRO90382.1"
                     /translation="MSTRIVVDLPDHVYRRIESMAQQSRRMVKDIVAEVVIQSVRSFP
                     VDANREDMLRETAAFHRLHPALLKEFPGQHVAILHGEIVDHDADPVALLKRIRSNYPD
                     QTVLRRKVEPAPEPILQFRSPRITIP"
     gene            6593..6982
                     /locus_tag="PLC06_07790"
     CDS             6593..6982
                     /locus_tag="PLC06_07790"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRO90383.1"
                     /translation="MNRLDFPYDEKYDPAMPVVEVVLDGYAGLDSKAVMAIIDSGADG
                     TMLPIDVLEALGALYQDTVQMRGVLGESERVDRYTVGLKLGAITLHGINSVAIPAGTE
                     SVIGRDVLNQLLLTLNGPAFTTQIEIE"
     gene            6988..8205
                     /locus_tag="PLC06_07795"
     CDS             6988..8205
                     /locus_tag="PLC06_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRO90384.1"
                     /translation="MCEGPRRSGVVWLLLAAAVVLVAVVYYRVVWGGVANLVVALDHC
                     RELYCDFTRQYYPTGQEILISGQPSKGYFYSSFFALLLAPFGRMALEPALAWWSVVQL
                     VSLLLLLIPAADFYHKSRAAALLYVALLAFSMPVLHNLKWGQVSTLMTGCVFAALYLN
                     ARGRWRGAAVVLGFAAAIKYYVAVFAWVHLVRRAWRFLLLLAVVVAVCWLVLPVSVLG
                     PGGSVAFYQTVSERMAHALATWLPEDINSQYLPSVAGRWLGTTSGRTVWQFAGYGLFA
                     LNALAVARYLRRQEASWAVDAWAYGLLFLALPFAINTSWPHYFVYLPFVQVLLWLRLR
                     QADGAGGQRARFQWALLIASIILASMPFFQLIGDWRLYSRAGVLFVANLCVLVAAHVD
                     RPAATPPPTPTYR"
     gene            8212..>9266
                     /locus_tag="PLC06_07800"
     CDS             8212..>9266
                     /locus_tag="PLC06_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRO90385.1"
                     /translation="MTQQHPDGVSRRNFLRFAGITVAAAAATGGGAAWLKRYEKAPVI
                     DTGVNGPPAYQSAPTFGDSPPVVSDIIPTLPAAGSDVYTQLAAAQAEIMQLRASNDQL
                     TRDLAAWQSAEGESRTQRDTLSLELDDARNRLSVLGGLVALYQQLDDADVGGILENGL
                     GAVGEKIGELIGGTPALSAGIAASELALGQVESHIPRLESGRAWVDAQEARLGGFYGE
                     VETRLQQAVERVGDFLALLADWFAGLRKWLPFGVGDKAADVMGALTSLLAETPATISG
                     LDDHVARPLDVWLSRIEGEPALTRILVRPVRDEVLVRARTTVDQAGTVATTWESYLAA
                     PTRAALANRQTLRQQIA"
BASE COUNT         1692 a         3035 c         2936 g         1603 t
ORIGIN      
        1 tgcaaaacgg ccttatggca tgcatcacgc atgtagctcc ggtttcctaa ccggatgatt
       61 gcccgatcgc ggcgccggat aagaatccgg cggtacatcg cagccggctg ctgaaaaaac
      121 ccgggccgac aggccgacag gtctcccgat tgatcatcct caccattccg cttataatta
      181 gcacatatgt tcttaattta gcgccctgtc atccagtctg atacaatcgc gacctatgaa
      241 attcaacgtt gccctggccc aaattgcccc caccctcggc gatgtcgccg ccaatctgga
      301 agcccacctc gtcgccatcg acgaagcgcg ggccgccggc gcggcgctga tcgtcttccc
      361 cgaactgtcg ctgaccggct acgcgctggg cgaccgggcg tgggatgtgg ccatccgccc
      421 cacggccgac gacccgacct tcctcaagct gctggccgcg tcggccaaaa tcgacatcgt
      481 cgccggcttc gtcgagatcg acgaccgcca tcgcctctac atcagcgccg catacctcgc
      541 cggcgggggg ctggtgcacc tgcatcgcaa gctctacctg cccacctacg ggctgttcaa
      601 cgagagccgc gtctacactc ccggccagtc gctgcgcgcc ttcgacaccc gcttcggccg
      661 cgtggggatg ctcatctgcg aggacatctg gcacgcctcg tccccctggc tgctgtggca
      721 ggacggggcc gacatcctca tcctcatctc ggcctcggtg gagcataatc agggcgaggc
      781 gctctccacg gcgggcaagg tgctgagcat gtgccgcgcc tacgcgctgc tgttcaccga
      841 tttcgtcgtc catgtgaacc agaccggcga ggacgacagc ggccgctatt ggggcaactc
      901 ggtcgtctat gggccggacg cttccttatt aatagaaggc ccgcgcgacg aggcggccgt
      961 cgtcacgggc gagatcgaca ccgcgctgct ggcccccgcc cgccgcgccc tgcccctgct
     1021 gcgcgacgag cgcgccgatc tggtggcgcg ggagttgggg cgaatattga tgatcgacgc
     1081 gggcatgtga cacccgcgtc cacacccact cacaaggcag attcaatcgt gacccaggaa
     1141 tacatcgaaa tccacaacgc acgcgaaaat aatctcaaga acgtctcctt gcgcgttccc
     1201 aagcgccaaa tcaccatctt caccggcgtg tcggggtcgg gcaaatcgtc gatcgtcttc
     1261 gacaccatcg ccaccgaagg ccaacggctg ctgaacgaga acttcagcat gttcgtgcgc
     1321 aacttcctgc cgcgcttcgg ccgccccgac gccgacgtca tcgccaatct gtctatgccg
     1381 gtcgtcgtcg accagaaacg gatgggcggc ggctcccact cgaccgtcgg caccatcacc
     1441 gacatctcgc cggtgctgcg gctgctgttc tcgcgggccg ggcaaccgtt cgtcggctac
     1501 tccaacgtct tctcattcaa cgacccgcag ggcatgtgtc ccgaatgcaa cggcctcggc
     1561 cgcaaactgg ctgtcgacat ggagcgtttc ctcgacacct cgctctcgct caacgaaggc
     1621 gcgctgctcg gccccggcta cgcggtgggc aaatgggact ggagcttcat cgtgcagtcg
     1681 ggtctgttcg atgtcgacaa gaagctcgac gactacagcg ccgaagagct ggagctgctg
     1741 ctccatagcc cgccgcgcaa ggtgcagacg gagttcggcg gccgaacgat caacctgacc
     1801 ttcgaggggg tcgtggccaa gttcacccgc aaatacatca aacaggacac ggccaatcat
     1861 tcggagcgca ccctgaagac ggtcgctccc tacatcaccg aaggcccctg ccccgcgtgt
     1921 catggcgcgc ggctcaacca ggcggcgctc ggctgccgca tcaacggcta caacatcgcc
     1981 gacctgcaat ccatggagat cgcccatcta atcgaggtga tccgggccat cgacagcccc
     2041 gtcaacgtca cgatgatcgc cacactgacc gagcgcctgc aacacctgat cgacatcggg
     2101 ctggaatatt tgaccctctc gcgccccacc gacaccctgt ccggcggcga atcgcagcgg
     2161 gtgaagatcg tcaagcagct cagcagcagc ctgatcgacg tgatgtacat cttcgacgag
     2221 ccgagcatcg gcctgcaccc gcgcgacatc catttgatga acgaactgct gcagaagctg
     2281 cgcgacaagg gcaacacggt gctggtggtg gaacatgacc cggcggtgat cgccatcgcc
     2341 gaccatgtgg tcgacgtcgg cccgcgggcg ggcgcggccg gcggcgaagt cgtctatgag
     2401 ggcaagctcc aggggctgct ccagtcgggc acgctcaccg gccgctatat gggacagacg
     2461 ctgcccatca aagcccaatt ccgcccgccg aaggggttgc tgcccatccg caacgcccgc
     2521 gccaacaacc tgcaaaatgt gagcgtcgac gtgcccgcag gcgtgttgac ggtcatcacc
     2581 ggcgtggccg gcagcggcaa aagctcgctc atcaaccaga ccttcctgaa gcgccacccc
     2641 gaagcgatcg tcatcgacca ggcggccgtg ggcgccaaca gccgctccaa cccggccacc
     2701 tacaccggca tcctcgacga cgtgcgggcc gccttcgcca gggccaacga ggtcagcgcc
     2761 tcgctgttca gcttcaactc cgagggcgcc tgcgaaaact gcaacggcgc gggcgtcatc
     2821 tataccgacc tgggcttcat ggacggggtc aagaccccct gcgagatctg cgaggggcgg
     2881 cgcttcaagg atgaggtgtt gacttacaaa ctcgacggga aaacgatcac cgatatactg
     2941 aatatgaccg cccgggaagc gctcgacttc ttcgacatcc ccaaagtgac cgtcaagctg
     3001 cgggcgatgg tcgacgtggg catcggctat ctgacgctgg ggcagccgct cagcacgtta
     3061 tcgggcggcg aatgccagcg catcaagctg gccggcgagc tgcacaagaa aggcagcatc
     3121 tatgtgatgg acgagccgac gaccgggctg cacatggccg acatcagcct gctgctggcg
     3181 atcatgaacc gcctcgtgga cgcgggcaac acggtgatcg tcatcgagca caatctggac
     3241 gtgattcgca acgcggactg gatcatcgac atggggccgg aaggcggcag caagggcggc
     3301 cgcgtcatct tcgagggcac gccgcaagcg ctgttggccg ccgaaggttc gctgacgggg
     3361 agatatttga atcatgacca tagccaaatc acgtagggac gggacaaccg gcgcgaacct
     3421 tcgtccgggc gacaaatcgt ggttagccgg ttgtctcgcc cctgttcgca gcaattgtaa
     3481 gggcgggttt ggacgacagg catccagtcg cacccgcccc tacccgacat tattattcca
     3541 aaggtgaacc catgctgatt cagcacaaat tctatatcaa cggccaatgg gtcgaggcgc
     3601 acagccggca gacggccgag gtcatcaacc cggccaccga agaagcgatc gcttccatcg
     3661 ccatgggcga cgagacggac gtagaccggg ccgtggccgc cgcgcgggca gcgttcgaca
     3721 actatgcgca atggtcggtg gccgagcgcg tggccctgtt cgagcggatc atcgccgtct
     3781 atgaccggcg catggccgag atggccgaga ctatctcgca agagatgggc gcgccgctgg
     3841 ccttcgccac catcgcccag gccccttccg gccgcgatca gttcaaggcc acgctggaag
     3901 cgctgaaaag cttcgaatgg gagaaagagg tcggccgcag ccgcgtcgtg cgcgagccga
     3961 tcggcgtttg cgccctcatc actccctgga actggccgca aaaccaggtc gtctccaagg
     4021 tcgcccccgc gctggccgcg ggctgcacca tggtgctgaa gccgtcggag atcaccccgc
     4081 tggacgccat cctgctggcc gggattctgg atgaggcggg cgtgccggcg ggcgtcttca
     4141 atctggtgaa cggcgacggg gccggggtgg gcacggcgct ggcccgccat cccgacgtcg
     4201 acatgatctc cttcaccggc tccacgcggg cgggggtgct ggtggcccaa aacgcggcct
     4261 ccacggtgaa gcgcgtgacg caggagctgg gcggcaaggc ggccaacatc atcctcgacg
     4321 acgcccggct ggagaaggtg gtcgccgggg gcgtgcgcgt ctgcgtccgc aacgcgggcc
     4381 agtcgtgcaa cgccccgacg cggatgctgg tgcccatgtc gcgcatggac gaggccgcgg
     4441 ccgtggcccg cgaggcggcc ctggccatcc gcgtgggcga cccgaccgcg gccgataccg
     4501 agattgggcc ggtcgcttcc aaaacccagt gggacaagat ccagaatctc attcgcaagg
     4561 ggatcgagga aggcgcgcgg ctggaatgcg gtgggccggg gttgcccgat gggctgaagc
     4621 gcggctacta cgtgcggccg accgtgttct cccatgtgga caaccgcatg gccatcgccc
     4681 gcgaggaaat cttcggcccg gtgctggtca tcatcggcta tgaggacgag gaagacgcga
     4741 tccgcatcgc caacgacacc ccctacgggc tgaccggcta cgtgcagtcg ggcgatctgg
     4801 agcgggcgcg gcgggtggcc cgccggattc gcgccggcag cgtccacctc aacggcgcgc
     4861 cgggcggcta cgacgtgcct ttcggcggct acaagcagtc gggcaacggc cgcgagcagg
     4921 ccggatgggg gctggaagag tatctcgaaa cgaaggcgat catcggctac taccctaaat
     4981 cataagagcc gggcacagga tgagcagtat gaacgacgaa caagaaccgg acatcatcga
     5041 agaaccaaac gacgtccacg acgcccacga cgcggcgcaa tacgaaaccc ccgaaggcca
     5101 ccgttccggc tttgtggcgc tcgtcggccg ccccaacgtg ggcaagagca cgctgatcaa
     5161 cgccttcatg cgccagaaga tcgccgccgt gacgccgcgg ccgcaaacca cgcgcacgcg
     5221 gcagctgggc atcatcaccg agccggacta ccagatgatc ttcatcgaca cccccggcat
     5281 catccgcgac ccgcgccacg atctggacag cttcatggtg caatcggcgc tggagacgct
     5341 cgacgacgcc gacgtggtgc tgtggttggt ggacatgagc gagaaccccg gcgcggccga
     5401 ccgcgacatc gccaaactgc tggccgaggg ggagcgcaag gtcattttgg cgctcaacaa
     5461 gatcgacgcc atcggcccga cggaggcgct ggcgcgaacc gaggcttatg tgtcgctgct
     5521 gcgctcggcc gaatggctgc acctgtcggc cttgaccggc gacggcgtgg cgatactgct
     5581 cgacaagctg gtggcggcgc tgcccgaagg gccgcgctat tatccggccg agcaaatcac
     5641 cgacatctat gagcgcaaca tcgccgccga gctgattcgc gagcagatca tgctgctgct
     5701 gcgcgatgag gtcccctacg gcacggccgt caaggtgcgc gatttcaagg agcgcgacaa
     5761 cggcagcacc tatgtgagcg ccgacatctt cgtggagcgc gacagccaca agagcatcgt
     5821 catcggcaag aacggcgcgc agctaaaggc catcggcgcg gccgcccgcg agcaaatcga
     5881 gcagctgacc ggcggcaagg tgttcctgga gctgtgggtg aaggtcgaaa aggactggcg
     5941 acgcgacccg aatctgctgc ggctgtttgg ctacagcaat gaatggacga aggagtgacg
     6001 agtgtcaacc acacgtcgag gcacgaacgg cgcgttctcc tttcgtcctt cgtacctcga
     6061 cggttggcga tggtgcgagg caccatacct cttgccacca aaatgaggta tcattttgct
     6121 accttttcag gtttttccgt agtaaggtgc ggagtcgttc cggcagcgac atcgctttcg
     6181 cttttaataa tggagaaata cggcccatga gtacccgaat cgtcgtcgat ttgcccgatc
     6241 atgtctaccg gcgcattgag tcaatggcgc agcaatccag gcgaatggtt aaagatatcg
     6301 ttgccgaggt cgtgattcaa tcggtgcggt cgtttccggt ggacgccaac cgcgaggata
     6361 tgcttcggga aacggccgct tttcatcgcc tgcatccggc gctcctcaag gagttccccg
     6421 gccaacatgt cgctatcctt cacggcgaga tcgttgatca tgacgccgac ccggtcgcgc
     6481 ttctcaagcg gatacggtca aattatcctg atcaaacagt cctgcgacga aaggttgagc
     6541 cggcccccga acccattctt caattccgct ctcctcgcat aacaattccc tgatgaatcg
     6601 tctcgacttt ccatacgatg aaaagtatga cccggctatg cctgttgttg aggtcgtgtt
     6661 ggacggatat gccggtttgg actcaaaggc agtgatggcc atcattgatt caggggctga
     6721 tggaaccatg ctgccaattg atgtcctgga ggccctcgga gcgctctatc aggatacggt
     6781 tcaaatgcgc ggcgtcctgg gggagagtga gcgagtagat cggtacactg tcggcctgaa
     6841 gctaggtgcg attacccttc acggcattaa ttccgtagcg attcccgcgg ggacggagag
     6901 cgtcatcggc cgcgatgtgc tcaatcaact tctgctcacg ctgaatggcc ccgccttcac
     6961 aacgcagata gaaatcgaat gaggaaaatg tgcgagggac ctcgccgatc gggggtcgtt
     7021 tggctgctgc tcgccgcggc cgtcgtcctg gtggcggtcg tctactatcg cgtggtctgg
     7081 ggcggcgtgg cgaatctggt ggtggcgctc gaccattgcc gcgagctgta ctgcgacttc
     7141 acccgccagt attaccccac cgggcaggag atactcatct ccgggcagcc gtcgaagggc
     7201 tatttctaca gctccttctt cgccctgctg ctggccccgt tcggccgcat ggcgctcgaa
     7261 ccggccctgg cgtggtggtc ggtcgtccaa ctggtgagcc tgctgctgct gctgatcccc
     7321 gcggccgact tctaccacaa gtcccgcgcg gcggcgctgc tctatgtggc cctgctggcc
     7381 ttctccatgc cggtgctcca caacctgaaa tgggggcagg tcagcaccct gatgaccggc
     7441 tgcgtcttcg ccgcgctcta tctcaatgcg cgcggccgat ggcgcggggc ggccgtcgtg
     7501 ctggggtttg ccgcggctat caaatattac gtggccgtct tcgcctgggt tcatcttgtc
     7561 cggcgggcgt ggcgcttttt gctcctgctg gccgtggtgg tcgccgtctg ctggctggtg
     7621 ctgcccgtgt cggtgttggg accgggcggg agtgtggcgt tctaccaaac ggtgagcgag
     7681 cgcatggccc acgcgctggc gacgtggctg cccgaagaca tcaactcgca atatctgccc
     7741 agcgtcgccg gccgctggct gggcaccacg agcggccgaa ccgtgtggca gttcgcgggc
     7801 tacggcctgt tcgcgctcaa tgcgctggcg gtggcccgct atctccggcg acaagaggcg
     7861 agttgggcgg tcgacgcctg ggcctatggc ctgttgttcc tcgccctgcc cttcgccatc
     7921 aacacctcat ggccccatta tttcgtctat ctgccgttcg tgcaggtctt gctgtggctg
     7981 cggctgcgac aggcggacgg cgcggggggc caacgggcac ggttccaatg ggcgctgctc
     8041 atcgcctcaa tcattttggc gtcgatgccc ttcttccagc tcatcggcga ttggcggctc
     8101 tacagccgcg ccggcgtcct gtttgtcgct aatctttgtg tgttggtggc ggcgcatgtt
     8161 gaccgcccag ccgcaacccc gccccccact cccacgtaca ggtgaaatga tatgacacaa
     8221 caacaccccg atggcgtctc gcggcgcaac tttttgcgtt ttgcgggcat tacagtggcg
     8281 gcggccgcgg cgacaggcgg cggcgcggcc tggttgaagc gatacgagaa ggcgccggtc
     8341 atcgacaccg gcgtcaatgg gccaccggct tatcaatccg cccccacatt cggcgactct
     8401 ccgccggtag tcagcgacat catccccact ctcccggctg ccggcagcga tgtctacacg
     8461 caattggccg ccgcgcaagc ggagatcatg cagctgcgcg cgtcgaacga ccaactcacg
     8521 cgcgatctgg cggcctggca gtcggccgag ggcgagagcc gcacccagcg cgacaccctc
     8581 tcgctggaac tcgacgacgc ccgcaaccgg ctgagcgtgc tgggcggcct tgtggccctc
     8641 taccagcagc tcgacgacgc cgatgtgggc ggcatcctgg agaacggcct cggcgcggtg
     8701 ggcgagaaga tcggcgagct gatcggcggc accccggcgc tgtcggcggg catcgccgcg
     8761 agcgagctgg cgctgggcca ggtcgagagt catatccccc ggctggagag cggccgcgcc
     8821 tgggtcgacg cgcaagaggc gcggctgggc ggcttctatg gcgaggtgga aacgcgcctg
     8881 cagcaggcgg tcgagcgggt gggcgatttc ctggcgctgc tggccgactg gttcgccggg
     8941 ctgcgcaaat ggctgccgtt tggcgtgggc gacaaggcgg ccgatgtgat gggcgcgctg
     9001 acctcgctgc tggccgagac gccggccacg atcagcggcc tcgacgacca cgttgcgcgg
     9061 ccgctggacg tgtggctgag ccgcatcgag ggcgagccag cgctgacgcg catcctcgtt
     9121 cgcccggtgc gcgacgaggt gctggtccgc gcccgcacca ccgtcgacca ggccggcacc
     9181 gtggccacga cctgggagag ctacctcgcc gcccccaccc gcgccgccct ggccaatcgg
     9241 cagacgctgc ggcagcagat cgccga
//
LOCUS       DAOMQG010000109         9253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3205_length_9253_cov_10.178952, whole genome shotgun sequence.
ACCESSION   DAOMQG010000109 DAOMQG010000000
VERSION     DAOMQG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_9253_cov_10.178952"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PLC06_07805"
     CDS             complement(<1..1068)
                     /locus_tag="PLC06_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRO90386.1"
                     /translation="MIPVQLGLRNFLSYRDPVELDLSGIHLACISGSNGAGKSTLLDA
                     ITWVLFGKSRVKSDDDLITRTEVAKGGIAEVSFVFELEGAIYRIIRRKAAGKSVELEF
                     HARATNDAAERWQVRTEAKVRETQAEIEKLLRMNYDVFTNASFLLQGKADEFTTKTPD
                     KRKEILAEILGVSQWDDYKDRATERRKATENDAGSIERQLAEVEAELAQEESLAQALE
                     LVEARAKTTTAERDRHEALVASARQNKAMADQQRELLRRLAAELGDLQRERARLDGIA
                     VQRRAELAGFQAILERREGIESAYREWTTADAGYATLQERAETHAAIVRQMHPIEMVI
                     ARAETQLEQRVKELEGQEEQAA"
     gene            complement(1065..2336)
                     /locus_tag="PLC06_07810"
     CDS             complement(1065..2336)
                     /locus_tag="PLC06_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRO90387.1"
                     /translation="MAVKILHFADAHIDIANYGRHDPETGLPYRVVDFLKSLDSIIDA
                     AIAERVDLVIFAGDAYKDRNPQPTFQRAWGERMMRLSRAGIATILLIGNHDIAPADYR
                     AHTLQEFATLAVPHVFVADKLMVFGPEQLGVPVQVLALPWVTRSRFLARNEMSGKTTD
                     EIHTAIVDRIRDGLDKTIANEIDPNLPLIFTAHASIGGATFGSERMVMLGQDLILSKG
                     LVANPRFDYVALGHIHKHQSLNEQPPVVYSGSIERIDFGEWREPKGFVLAEVERGKPT
                     SWRFVELETRPFYDKLIRLEEADGFMDQLMSKLPAQEQIEGAICRLRLEYPHELDALL
                     DEKLIYEHFAGAFDLRIVKNRTGGNTSRISRLIAVESLGPYELLRLYWGSKNHNDGEV
                     NELLTLAEEVLGIHAPTAGALAELNLDDISQ"
     gene            complement(2358..>4407)
                     /locus_tag="PLC06_07815"
     CDS             complement(2358..>4407)
                     /locus_tag="PLC06_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRO90388.1"
                     /translation="GWACYYWPHMRSFLVALFVIVTWVVVATGAWADDRSPQVDDQPL
                     IVQPPENWIGTEIKVTDLPLSPQAPDAAPAADQCSAATPLELSFRNTADGSGTLTNLF
                     SQEATDPVLSCMFGAPTSPQGYRTAWYKLVAGDTSTVTITTEGTDYDTVLAVFTGSCD
                     ALQSLACSDDFRSFQSSATFPVTRGHTYYIQVADYHSGAPGAAFLQLSAVLREGGERW
                     NQASTMPFGGITRHALVSDGPEMFIIGGQTRVAGGAEISNRFLLYNALGDSWRELSDI
                     PGPGLSNTTAVRLGRKIYIPGGFNGDQSNYANTHYVYDIATDYWEQIASIPQALLPNG
                     EMFAWSAAVPAPGHLAYYVTGGITNYPSSDPDPDPDAVVINNVYRFVPATNQWEPSPP
                     MLAPRYAHTAAWVFAGNRGLCVAGGLDVGEDDEGDPVTVLLTTGECFNPTTGSWQPTG
                     DLNFPRYNAGSAIGPDGNWYIFGGLDANGGVPETEFYNPLTNSWQVLGGEFSLGGLPQ
                     NPARVWPRGAFWGDRLFVFGGNTPPREYRVISAVEKMSIGAGFAPLAKTIVFPLVASL
                     GAENLLNNAIGLPPNTPLSGNFSSSTQFYNPYFFDWPAFGRARIVLSNIPEDSNFNIS
                     VYDSNKVLRGRGNTTLYGGEKTVSLTLAPGRYYVVAERIFPKDLPNPAVYYRLTLSRQ
                     "
     assembly_gap    4408..4507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4657..>6770)
                     /locus_tag="PLC06_07820"
     CDS             complement(4657..>6770)
                     /locus_tag="PLC06_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRO90389.1"
                     /translation="DIFTQLRETGFFYRKQRYAHRYPHCWRCGSELVYRLVDEWFISM
                     GQSYDKPRAELTAAEKSGSLRYQIMDRVDRIDWFPSFGYDREMDWLRNMHDWMISKKR
                     YYGLALPIWECDECGHFTVIGDEHELRERAIEGWSEFEGHTPHRPYIDAIKIACPECG
                     AKASRIADVGNPWLDAGIVAFSTLGYRQNRAYWDKWYPADLITESFPGQFRNWFYSVL
                     AQSTVMTEQPPFRNLFGYATLFAEDGREMHKSWGNSIEFNEAAERMGADTMRWLYAGC
                     KPEQNLLFGYTRGDETRRRFLIPLWNVYSFFVTYANLDGWLPNGSHRDFRSPVEGNAQ
                     LDQWIVARLDETTVDVRAALDRWDAERAVADLEMLLDDLSNWYVRRSRRRFWKSETDQ
                     DKQAAYATLYHVLVEFVKLLAPFIPFVTEEMYQNLVCSVDDSRPASVHHTLYPQVDER
                     ALDHRLLDKMRLAITAAALGRSARGTAGKLRQPLARAKVFVGSQQERDDLLELADVLA
                     EEINVKEIEIVSEVGELVSYRLLPNNRVLGPKLGPLFPEVRNALAALDPAVAAATLQS
                     GGTLDLTINDQTLSLGGDDILVQTEPLGGLAVAGEKGVTVAVDPHLTPALIREGYARD
                     LVRGINDMRKKAGLDISDRIELGYEAEGEVAAAIKDFAAYIGEETLAVGLVSGGVKDP
                     AYNQSIEVGDEQVRITLRKAN"
     assembly_gap    6771..6870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6871..8198)
                     /locus_tag="PLC06_07825"
     CDS             complement(<6871..8198)
                     /locus_tag="PLC06_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRO90390.1"
                     /translation="MGFKEVNNKVDFVALERDILEFWRQTDAFNELRRLRAQSEAENG
                     IFSFIDGPITANNPMGVHHAWGRTYKDLYQRYYAMQGKNLRWQNGFDCQGLWVEVNAE
                     KELGFTSKRDIEKYGLANFVKYCKSRVLRYAAVQTEQSRRLGYWMDWNDPDVLRRLGE
                     LLNEDPGQVITLDGPQGPVTDTVEQLVGQLGLPELGGSYFTFSNENNYQIWGFLKKCH
                     EHGWVYKGEDVMPWCPRCGTAISQHEIVTDGYFEVTHDSVFVRFPLVGRENEALLVWT
                     TTPWTLSSNVAAAVGPDLDYVKVRAEDGWTYYLAEGAMKNTLLGKGNEVIGRMKGEDL
                     VGWEYVGPFDDLPRVQAAFAEAGYTHRVVPWREVGAEEGTGIVHIAPGCGAEDYMLSK
                     EHNLPVIAPLDENGIYVAEFGWLSGREVAGVAEDIFTQLRETGFFYRKQR"
     gene            complement(8229..8891)
                     /locus_tag="PLC06_07830"
     CDS             complement(8229..8891)
                     /locus_tag="PLC06_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90391.1"
                     /translation="MRFIIHEMPYERPLLAGRLRYERDGVPTGAVELWRITNAVDGYR
                     FLRVDLDAREAESGRSSLYHVTLNPAGRPEQLKFRLWGNGPEVSGSLVWNQGEIIAAR
                     QVDDVAYQDVARDRAFWFPAGSGLALLSLSVGESTGVTMSAAAAEPSELMKLVEMPVM
                     IELGEAILEDVAGELLPVRPLQVSWKEQGRTVWIDPEGRPLRLLRDDGLTAVAERLVR
                     YS"
     gene            complement(8891..>9253)
                     /locus_tag="PLC06_07835"
     CDS             complement(8891..>9253)
                     /locus_tag="PLC06_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRO90392.1"
                     /translation="GGNMDPLQGRMDFEFETMRHELVAAIEEAVNAEVAAARPVRVRI
                     LPREEAFAIPDLIRTKINLLPEGITEIRTVELVGLDLQADGGTHVANTSEVGRIRITD
                     YKSKGKINKRIYLELEAN"
BASE COUNT         1910 a         2812 c         2487 g         1844 t
ORIGIN      
        1 cgccgcttgc tcttcctgcc cctccaattc cttgacgcgt tgctccagtt gcgtttcggc
       61 gcgagcaatt accatttcaa tcgggtgcat ttggcgcacg atagccgcgt gcgtctcagc
      121 ccgttcctga agggtcgcat agccggcgtc ggccgttgtc cattcccggt aagcgctctc
      181 aattccctcg cgtcgttcaa gtatcgcctg gaagccggca agctcggccc gacgctgaac
      241 agctattcca tcgagtcgcg ctctttcccg ctgcaaatcg cccagctcag cggcgagccg
      301 gcgcagcaat tcccgttgct ggtcggccat ggccttgttt tgtcgcgccg aagcgaccag
      361 tgcctcatgt cgatctcgct cggccgtggt cgtttttgct cttgcctcaa ccagctccag
      421 cgcctgagcc agactttctt cctgggccag ctcggcctcg acctcggcca gttgtcgctc
      481 aatcgacccg gcgtcgttct ccgtagcctt tcttcgttcg gtcgcccgat ccttgtaatc
      541 gtcccactgg ctcacgccaa ggatttcagc caggatttcc ttgcgtttgt cgggcgtctt
      601 ggtggtgaac tcatccgcct tgccctgcag caaaaagctg gcgttggtga agacgtcgta
      661 gttcattcgc aggagcttct cgatctcagc ctgcgtctcc cggaccttgg cctcggtgcg
      721 cacttgccag cgctcggccg cgtcattggt agcccgtgca tgaaactcaa gctcaaccga
      781 cttccccgcc gccttgcgcc gaatgatccg gtagatcgcg ccctcaagct cgaagacaaa
      841 gctcacctcg gcgatgcccc ctttggcgac ttcggtacgc gtgatcagat catcatcgct
      901 cttgacgcgg ctcttgccga acaataccca ggtgatggca tcgagcagcg tgctcttgcc
      961 cgcgccgttg gagccggaga tgcaggccag gtgaatgccg ctgagatcca gttcaaccgg
     1021 atcacgatac gagagaaagt tgcgcaggcc aagttgaacg ggaatcattg ggaaatatcg
     1081 tccaggttaa gctccgccag ggcgccggcg gttggcgcgt ggatgccgag tacctcttcg
     1141 gccagcgtca ggagctcatt aacctcacca tcattgtggt tcttgctgcc ccaatacagc
     1201 cgcaacaact catacggccc aagggactca acggcaatca gccggctgat tctggaggtg
     1261 ttgccgccgg tgcgattctt gacgatacgc agatcaaacg cgccggcgaa atgctcataa
     1321 atgagctttt catccagcag cgcgtcgagt tcgtgcgggt attcgagtcg caagcgacaa
     1381 atggcaccct cgatttgttc ctgggccggc aatttgctca tcagttggtc catgaagccg
     1441 tcggcctctt cgagccggat gagcttgtcg tagaaaggcc gtgtctcaag ttcgacgaaa
     1501 cgccatgagg taggtttgcc tcgctccacc tcagccagaa caaacccctt gggctcgcgc
     1561 cattcgccaa agtcgatccg ttcaatcgag ccggaataga ccaccggggg ctgttcattg
     1621 agcgactggt gtttatgaat atgacccaat gcaacataat cgaagcgagg gttggcgacc
     1681 agacctttgc tcaaaatcaa gtcctgcccc agcatcacca tccgctcgct gccaaaggtt
     1741 gcgccgccaa tgcttgcgtg ggcagtgaag atgagcggca agttggggtc aatttcgttc
     1801 gcaatggtct tgtccagccc atcgcgaatc cggtcaacga tggcagtatg gatctcatcc
     1861 gtggtcttcc ccgacatctc gtttcgtgcc agaaatcgac tgcgggtgac ccacggcaga
     1921 gccaacacct ggaccggtac gccgagctgc tccggcccaa ataccatcag cttgtcggcc
     1981 acaaacacgt ggggcacggc caacgtggca aactcttgca gcgtatgcgc gcgataatcg
     2041 gccggggcaa tatcgtgatt gccgatgagc aaaatcgtcg cgattccggc ccgcgagagc
     2101 cgcatcatcc gctcccccca cgcgcgctga aatgtcggtt gcgggttgcg atccttgtag
     2161 gcatccccgg caaagataac cagatcgacg cgctccgcaa tcgccgcatc aatgatgctg
     2221 tctaatgatt tgaggaaatc gacgacgcga tagggcaacc ccgtctcagg atcatgtcgg
     2281 ccgtagttcg cgatatcgat gtgggcgtcg gcaaaatgca gaattttaac ggccataagc
     2341 ggtgtaaata agcgatgtta ttgacgcgac aacgtcaggc gataataaac ggcaggatta
     2401 ggcaagtcct tcgggaagat gcgctcggca actacatagt aacggccagg ggccagcgtc
     2461 agggaaacgg tcttctcgcc accgtagagc gtcgtattcc ctcggccgcg cagcaccttg
     2521 ttcgaatcat agacggagat attgaagttg ctatcttccg ggatgttgct caggacaatt
     2581 cgggcacggc caaacgcggg ccagtcaaag aaataggggt tataaaactg ggttgaactg
     2641 gagaaattac ctgagagcgg cgtattcggc ggcaggccga tggcgttgtt caacaaattt
     2701 tccgcgccca acgatgcaac aagcggaaag acgatcgttt tggccagcgg cgcaaatccg
     2761 gcaccaattg acattttctc aacggccgag ataactcgat attcccttgg gggcgtgttg
     2821 ccgccaaaga caaaaagccg atcgccccaa aaggccccac gaggccaaac gcgcgccgga
     2881 ttttgcggca gcccaccgag actgaattcg ccgcccagaa cctgccagct gttcgtgagc
     2941 gggttataaa attcggtttc gggcacgccg ccgttggcgt caagcccgcc gaaaatgtac
     3001 cagttcccat ccggcccaat agcgctgccc gcattatatc gcggaaaatt cagatcaccg
     3061 gtcggctgcc agctacccgt ggttgggttg aagcactcac ctgtcgtgag caggacggtc
     3121 accggatcgc cctcgtcatc ttcgccgaca tccaggccgc cggccacgca caagccccga
     3181 tttccggcaa acacccaggc ggccgtgtgc gcatagcgtg gcgccaacat gggcggggaa
     3241 ggttcccatt ggtttgtggc ggggacaaaa cggtagacat tgttgatgac caccgcatcg
     3301 ggatcgggat ccgggtccga tgaaggataa ttggtaatgc cgcccgtcac ataataagcc
     3361 agatgacccg gcgcgggcac ggcggccgac caggcgaaca tttcgccgtt gggcaacaag
     3421 gcttgcggga ttgaggcgat ctgctcccag taatcagttg ctatgtcgta gacatagtgc
     3481 gtattcgcat aattcgattg atcgccgttg aaaccaccag ggatatagat cttccgcccc
     3541 agacggacgg ccgtcgtatt cgatagccct gggccgggaa tgtcggatag ctcccgccag
     3601 ctatccccaa gcgcgttata tagcaagaat ctgttgctga tttccgcccc accggccacc
     3661 cttgtctggc caccaatgat aaacatttcg gggccgtcgc tgacgagcgc gtgacgagtg
     3721 atgcccccaa acggcatcgt gctcgcctga ttccaccgct cccctccctc gcgcaagact
     3781 gcggatagtt gcagaaaggc tgcgcccggt gcgccggaat gatagtcggc tacctggatg
     3841 taatatgtgt ggccacgcgt aaccggaaat gtggcgctgg actggaagct gcgaaaatca
     3901 tcgctgcaag ccaatgattg caaggcatcg cagcttccgg tgaagacagc caacacagta
     3961 tcgtaatcag tgccttcagt cgtgatggtg actgtgcttg tgtcgccggc taccagttta
     4021 taccaggcag tgcgatagcc ctgaggacta gtcggagcgc cgaacatgca ggacagaacg
     4081 ggatcagtgg cctcctggct gaagagattg gtcaacgtgc cgctgccatc ggccgtgttc
     4141 cggaaagaaa gctccagggg ggtggccgca gagcattggt cggccgcggg cgctgcatcc
     4201 ggcgcttggg gagacaaggg aagatcagtt accttaatct ccgtgccgat ccagttctcg
     4261 gggggctgaa caatgagggg ttggtcatcg acttgtggag aacgatcatc cgcccacgcg
     4321 ccggtagcta ccactaccca cgttacgatt acgaatagag caaccagaaa ggaacgcata
     4381 tgcggccaat aatagcaagc ccaacccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnngaa tagagcaacc agaaaggaac gcatatgcgg ccaataatag caagcccaac
     4561 ccgaaaaggc acatggctta atggagcggg cagcgacagg tggatcaaat atcgacgggc
     4621 gaggcggaga atcctcctcc acgcccgtcg atatagttag tttgccttgc gcaacgtgat
     4681 acgaacctgt tcgtcaccga cctctatgga ctgattatag gcaggatcct tcaccccacc
     4741 cgataccagg ccgacggcca acgtctcctc gccgatatag gcggcgaaat ccttgatggc
     4801 cgcagcgacc tcgccctcgg cctcataacc cagttcgatc cggtcggaaa tatccagtcc
     4861 cgcctttttg cgcatatcat tgatgccgcg gacgagatcg cgggcgtatc cctcgcgaat
     4921 caatgccggc gtcaggtgtg gatcgacagc caccgtcact cccttctcgc cggcgacggc
     4981 aagcccgccc agcggttccg tttgcaccaa aatgtcgtca ccgcccagac tgagggtctg
     5041 atcattgata gtcagatcga gcgtgccgcc ggattgcaaa gtagcggccg cgacagccgg
     5101 atccagtgcc gccagcgcgt tgcgcacttc agggaatagc ggcccaagtt tcgggccaag
     5161 cacacgattg ttgggcagca ggcgatagct gaccaattcg ccgacctccg agacgatctc
     5221 gatctccttg acgttgatct cttccgccag cacgtcggcc aactccagca aatcatcacg
     5281 ctcttgctgc gagccgacga acaccttggc ccgcgccagg ggttggcgca gtttgcccgc
     5341 cgtcccgcgt gccgaacgtc ccagtgcggc agcggtaatc gccaggcgca tcttgtccag
     5401 caagcgatga tcgagcgcgc gctcatcgac ttgcggatac agcgtgtgat ggacgctcgc
     5461 cggccggctg tcgtccacgg aacaaacaag gttctgatac atctcctcgg tgacgaaggg
     5521 gatgaacgga gccaacagct tcacgaactc aaccagcacg tgataaagag tcgcataggc
     5581 cgcttgctta tcctgatcgg tttcgctctt ccagaaccgg cggcgggaac ggcgcacata
     5641 ccagttcgac agatcatcga gcagcatctc aagatccgcg accgctcgct cggcgtccca
     5701 acgatcgaga gcggcacgca catcaaccgt ggtttcgtcc agtctggcga cgatccattg
     5761 gtcaagctgg gcgttcccct cgacgggtga gcgaaaatcg cgatgcgagc cgttgggcag
     5821 ccagccgtcc aggttggcat aggtgacaaa aaacgaatag acattccaca acgggatcag
     5881 gaatcgccgc cgcgtctcgt cgccgcgcgt ataaccgaac agcaggtttt gctccggctt
     5941 gcacccggca tagagccagc gcatggtatc ggcgcccatt cgctcggccg cctcgttgaa
     6001 ctcgatggag ttgccccagg acttgtgcat ctcgcggccg tcttcggcaa agagtgtggc
     6061 ataaccgaac agattgcgga aaggcggctg ttcggtcatc accgtgctct gggccagcac
     6121 actatagaac cagttccgga attggccggg gaagctctcg gtgatcaggt ctgccgggta
     6181 ccacttgtcc caatacgccc gattttgccg atagcccagc gtgctgaaag caacgatgcc
     6241 cgcgtcaagc caagggttgc ccacatcggc gatgcggctc gccttggccc cgcattccgg
     6301 gcaggcgatc ttgatggcat cgatgtaggg tcgatgcggg gtgtggccct caaattcact
     6361 ccaaccttca atggcgcgct cgcgcaactc atgctcgtcg ccgatcaccg tgaagtggcc
     6421 acattcatcg cactcccaga tcggaagcgc caggccataa tagcgcttct tgctgatcat
     6481 ccagtcgtgc atattacgca accagtccat ctcccggtcg tatccgaagg atggaaacca
     6541 atcgatccgg tctactcgat ccatgatctg atagcgcaga ctgccgctct tttcggccgc
     6601 agtcagttca gcgcgtggtt tgtcatagct ctgtcccatg ctgatgaacc attcatcgac
     6661 gaggcgatag accagctcgg acccgcagcg ccagcaatgg gggtatcgat gggcgtatcg
     6721 ctgcttgcga tagaagaacc cggtctcgcg cagttgggtg aaaatatcct nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tatcgctgct tgcgatagaa gaacccggtc
     6901 tcgcgcagtt gggtgaaaat atcctcagcc acaccggcca cttcccgacc gctcagccag
     6961 ccaaattcgg ccacatagat cccgttttcg tccaatggcg cgatgacggg aaggttgtgc
     7021 tccttgctca gcatgtagtc ttcagcgcca cagcccggcg cgatgtggac gatgccggtg
     7081 ccttcttcgg cccccacctc gcgccacggg acgacgcggt gggtatagcc cgcttcggcg
     7141 aaagcggcct gaaccctggg caagtcgtcg aatggcccga cgtactccca accaacgaga
     7201 tcttcgccct tcatgcgacc gatcacctcg ttacccttgc ccaacagagt gttcttcatc
     7261 gccccttcag ccaaatagta cgtccagcca tcctcggctc tcaccttcac gtaatccagg
     7321 tcaggcccaa cggccgcggc gacgttgctc gacaacgtcc agggggttgt cgtccagacc
     7381 agcaaggcct cattctcgcg gcccacgagc gggaaacgga caaagaccga gtcgtgggtc
     7441 acctcgaaat agccgtcggt gacgatctcg tgctggctga tcgccgtgcc gcatcggggg
     7501 caccacggca tcacatcctc ccccttatac acccagccat gctcatggca tttcttcagg
     7561 aagccccaga tctggtaatt attctcattg ctgaatgtga agtaggagcc acccaattca
     7621 ggcagaccca attgcccaac cagctgctcg accgtatcgg tcacaggccc ttgtggtcca
     7681 tcgagcgtga ttacctgacc gggatcttcg ttcagtagtt cacccaggcg ccgcaacacg
     7741 tcgggatcgt tccaatccat ccagtagccg agacgccggg attgctccgt ctgcacggcc
     7801 gcatagcgga gcacgcgcga cttgcaatat ttcacgaaat tcgccaggcc atacttttcg
     7861 atgtcgcgct tgctggtgaa acccaactct ttctcggcgt tgacctccac ccatagcccc
     7921 tgacaatcga acccgttttg ccagcgcaaa ttcttgccct gcatggcata gtaacgctgg
     7981 taaaggtcct tgtaggtccg cccccaggca tggtgaacgc ccatcggatt attggccgtg
     8041 atcggcccgt cgatgaaact gaagatgccg ttttcggctt cactctgtgc ccgcaagcga
     8101 cgcagctcat taaatgcgtc tgtctgccgc caaaattcca ggatgtcccg ctccaaggct
     8161 acaaagtcga ccttgttgtt gacctcttta aaacccatgt ttctctcctc ataacctcat
     8221 acgttcgttc agctgtaacg cacgagacgc tcggccacgg ccgtcaatcc atcatcccga
     8281 aggaggcgaa gcggccgccc ttcgggatcg atccagaccg tccggccctg ctccttccag
     8341 ctcacttgca acggcctgac cggcaaaagt tccccggcta catcctccag gattgcctcg
     8401 cccagttcga tcatgaccgg catttcgacc aatttcatta gttccgatgg ctcggccgcg
     8461 gcggcactca tcgtgacccc ggtcgactcg ccaacgctca acgacaacag cgcaaggccg
     8521 gacccggccg gaaaccagaa tgctctatct cgagcgacat cctgataggc aacatcatcc
     8581 acctgtctgg cggcgataat ctcgccctga ttccacacaa gtgagcctga cacttcgggg
     8641 ccattacccc acaggcgaaa cttgagttgt tcaggccggc cggcagggtt caaggtcacg
     8701 tgatacagac tcgaacggcc ggattccgcc tcgcgcgcat ctaaatcaac acggagaaac
     8761 cgatagccat caaccgcgtt cgtgatgcgc cacaattcga ctgcgccggt gggcacaccg
     8821 tcccgctcat agcgcaaccg cccggccaat aacggccgct catacggcat ctcatgaatg
     8881 atgaagcgca ctaattcgcc tccaactcca gatagatccg cttgttgatc ttgcccttgc
     8941 tcttgtaatc ggtgatacga atgcggccaa cttcgcttgt attggcgacg tgggttccgc
     9001 cgtcggcctg caaatcaagc ccgaccagtt caaccgtccg aatctcagtg atgccctccg
     9061 gcagcaggtt aatcttggtc cggatgaggt cgggaatggc aaacgcctct tcgcggggca
     9121 aaatgcgcac gcggacgggc cgcgccgccg cgacctccgc gttgactgcc tcctcgatgg
     9181 cggccacaag ctcatggcgc atcgtctcga attcgaagtc cattcgccct tgcagcgggt
     9241 ccatattgcc gcc
//
LOCUS       DAOMQG010000110         9181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3238_length_9181_cov_8.502301, whole genome shotgun sequence.
ACCESSION   DAOMQG010000110 DAOMQG010000000
VERSION     DAOMQG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_9181_cov_8.502301"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..89
                     /locus_tag="PLC06_07840"
     tRNA            8..89
                     /locus_tag="PLC06_07840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Leu,seq:caa)
     gene            184..519
                     /locus_tag="PLC06_07845"
     CDS             184..519
                     /locus_tag="PLC06_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90393.1"
                     /translation="MRWAAILGLVIGIGLGLLAGWVIWPVEYVDVTPDLLTADAQIDY
                     ALMVATAYEADGNLELARGRLARLGDSASVALLNAFTAAEDNQIALSGLQKLAAELEL
                     HATMPTPME"
     gene            524..1291
                     /locus_tag="PLC06_07850"
     CDS             524..1291
                     /locus_tag="PLC06_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90394.1"
                     /translation="MRRPPVLLIFLVFCVAGIMAGLAFAWLVAPMQPAGVTPDRLSPT
                     ERELVIRLIADSFAADGDRDAAARRLNGLGPAAEGTLVEMIASDLTKERPSVTANNLI
                     GLAEGLTIGAPIVKLLARPAGIPSATPSASSVPTSADITATPAAKGQFELTERVVFCS
                     AGDDADRIEINIIGNNGQPQAGVAITVFWDDGRDSFFTGFTPNQGPGYADFTMKQGAI
                     YSVAIAEDEVSVAGLEPQLCADGRMGGWRLEYRARES"
     gene            complement(1292..3085)
                     /gene="mutL"
                     /locus_tag="PLC06_07855"
     CDS             complement(1292..3085)
                     /gene="mutL"
                     /locus_tag="PLC06_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRO90395.1"
                     /translation="MTIHVLPDQLASQIAAGEVVERPYSVVKELVENAIDAGATAITI
                     DIRAGGRELIQVADNGDGIPAGEIEVAFHRHATSKLTSADDLWVIRTLGFRGEALAAI
                     SAVSRLTVVSRARESASGVRLLLEGGMTAAREIVGAPVGTVIAVENLFYNVPARLKFL
                     KSVTTEKRLIDEFVTRYSLAYPEIRFRLSHDGRITYQTTGSGATADVLLAVYGPDTTR
                     QLIEVLPPESGAAHGDGHRYKIDVHGYVSDPALTWSGRNHIQLFVNGRTIRDTRLTFA
                     VVQAYHTLLPVGRFPLALLFIELPPEEVDVNVHPTKVEVRFRDEGAVFGAVQRAVRAA
                     LVETSPVRGASGWPFGSSSPSGWGSFRPGSDTDGMPSTQLDIPWQLRGPEPERADGDI
                     DSPAVPLPGTDSSRLPIMRVIGQVGAAYIIAEGPDGLYLIDQHAAHERILYEQFLAQR
                     QGQRVASQGLVAPATVYLSPSQSHLVEEQSDLLASLGFEIESFGPNAFRMRAVPAILS
                     RADPAAALAAVVADLENETTPLKSETEALVIKRVCKTAAVKAGQTLSTQEMEAMILQL
                     EACDVPHTCPHGRPTLIHLSAALLARQFGRT"
     gene            3202..4083
                     /locus_tag="PLC06_07860"
     CDS             3202..4083
                     /locus_tag="PLC06_07860"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRO90396.1"
                     /translation="MPFQPTYDVQPGRMPYTLKPAATASGRQTGVLILHGFIGSPLSS
                     RPMAEYLNRQGFFAHCPLLPGHGSYPNKLHGISSKAWLAEVEEAFQFARGECDDLFIV
                     GHSMGNVLAAHVAIKFGGVRGVAMLAPVIDVPDSRLRWVKYVRHFVPWYYPHKSRSES
                     MQHLVRERVLDFDPTIDFDSPEFQEQLPEVSRVPLSGMHGMVEMIEKGRSLWPKLDVP
                     VRIFAGEDDNAAPPDNARRIFALLPNADKKLTIYPDVGHELMRPFEPIHKTVWESIYS
                     FIIEHTPPSGKTENNEE"
     gene            4086..5468
                     /locus_tag="PLC06_07865"
     CDS             4086..5468
                     /locus_tag="PLC06_07865"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HRO90397.1"
                     /translation="MSDYVTFPKGFVWGAATAAYQIEGAWNEDGKGESIWDRFTHTPG
                     NIIDGSTGDVACDHYHRWPEDIALMKSLGLQAYRFSIAWSRVLPDGRGKVNPAGLDFY
                     SRLVDGLLEAGIAPFVTLYHWDLPQALQDEGGWPERTTAEAFVEYADIVSRRLGDRVK
                     RWITHNEPWCASILSHQYGEDAPGWRDTAAAVRAAHHLLLSHGWAVPVIRANSPDSEV
                     GITLNFEAIVSASESPADRRLTRIEDGTFNRWFISPLFGRQYPADAMAHYEPYFPQGM
                     EFIKPGDFDAIAAPMDFLGVNYYMRRIVRDESAADNWPQTLFPDLPKCDMFSGTDDPV
                     YWEIYPEGLYNLLNRVYFEYKPPKIYITENGTSFMDVPDKNGRISDSRRIGYLRDHFA
                     ATHRAMQNGIPLSGYFVWSLMDNFEWAKGYQQRFGMVWVDYETQQRTPKDSARWYSEV
                     IANNGFASAD"
     gene            5486..7891
                     /locus_tag="PLC06_07870"
     CDS             5486..7891
                     /locus_tag="PLC06_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRO90398.1"
                     /translation="MQTNPTPSTTPNNPGSNAAVLPILLLAAVVGGLLAVILPAVWIA
                     EQFAIIGGETPGRGSWLVAHGVLALSILLPSLVLAAVVKTPRMRAVFRTWSLAGLFAL
                     IMLPVQLVPGPSAQTAALVQIGCAAAFLLIMRLIRRRNAAITSTAAGSLWPVILLAAL
                     IGWPWVLWGALGSWLDTTLNLLASALFGLAAAATLRHELWSHINPNQGNPPLPFFIAG
                     VATSIALMVMGAALGHNGQQLVLLALMAGLGWLVAAVGRRGRGVESAIRPQATLLIGL
                     VVALPLLFFDPEELALVLNLGSRDVGYFVFYATLVGLGMALGLAIIVWITRNTGGGRA
                     IPALLATGALAGLAAIYVFVGQPGWHGERLYVVMADQADLSAAVDIVDPIERRQYVYE
                     RLTDHANATQAGVRQALDAVGVNYRPYYLVNALEVDAGPLVRLWLAAQPEVDRVLLSP
                     ELRPLPEQPPVTTGSATGPDEPEWNLVDIGAPQVWQAFGARGAGIIIGQSDSGVDGAH
                     PELSAQYRGAQAASPTGNDYNWYDPWNASPSPTDIGGHGTHTLGSILGRTVGVAPDAS
                     WIGCVNLGRNLGNPPRYLDCLQFHLAPFPQAGDPLRDGRPEWGAHVLNNSWGCPEIEG
                     CDPTSLLAAVRALRAAGVFVVASAGNDGSACSTIADPISLYDEVFTVGAIDEFGAIAP
                     FSSRGPVLADGSGRIKPDIVAPGMGVLSAMPGGTYEYNSGTSMAGPHVAGVVALLWSA
                     DPALIGDIDRTERILAQTAVPLDELPGLYACGNPGEIPSNVSGYGLINAYAAVKLALS
                     ENE"
     gene            complement(8038..>9181)
                     /locus_tag="PLC06_07875"
     CDS             complement(8038..>9181)
                     /locus_tag="PLC06_07875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRO90399.1"
                     /translation="IDPAEVEEVYFGCANQAGEDNRNVARMGALLAGLPHSVGGVTMN
                     RLCASGLNAVNQAARAIRSGEGDIYIAGGVESMTRAPYSLPKNSRAFGPSGNVTAWDT
                     TLGWRYPNPKMQALFPLEAMGETAENIYEQSVAGAISGGVISREDQDAFALESQRRAM
                     EAINRGYFREEIVPVVFPQRKGDPIVFDTDEHPRIRKGEGGYVLDTSMEVLSKLKPAF
                     RQGGTVTAGNASGLNDGAAAVLLMSAGRAEALGLKPMARWIGSAAAGVDPRTMGLGPV
                     PATRKALARVGLTIDDIDLIELNEAFAVQSLAVMRELGMRPEITNVNGGAIALGHPLG
                     CSGARILATLLHEMRRRRHAGESMRYGLATLCVGVGQGEATIVELI"
BASE COUNT         1652 a         2971 c         2837 g         1721 t
ORIGIN      
        1 aagccatgcc gaagtggcgg aacaggcaga cgcgcacgac tcaaaatcgt gttccgaaag
       61 gagtgtgggt tcgattccca ccttcggcat caagctgaca taattatagg tctcgccccg
      121 aaggcgggca agcgatggct ccgttcggga gccgttttca tttaggcgcg aggtggctta
      181 aggatgcgat gggcggcaat attggggctg gtgatcggta tcggcctggg ccttctggcc
      241 ggttgggtga tctggccggt agaatacgtt gacgtcacgc ccgatctgct gaccgcggac
      301 gcccagatcg attatgcgct catggtcgcc accgcgtatg aagccgacgg caatctggaa
      361 ctcgctcgcg gccggctcgc ccggcttggc gattcggcgt cggtcgccct gctcaacgcc
      421 ttcaccgccg ccgaagacaa tcaaatagcg ttatccggct tgcagaaatt ggcggccgaa
      481 ttggagctgc acgccaccat gccgactccc atggaataag ctcatgcgcc gtccccctgt
      541 cttgttgatc ttccttgttt tttgcgtcgc cggcatcatg gccgggctgg catttgcctg
      601 gctggtagcc cccatgcaac cggccggcgt aactccggat cgcctaagcc caaccgagcg
      661 cgaactggtt atccggctca tcgccgacag ctttgccgcc gatggcgatc gggatgcggc
      721 cgcgcgtcgt ctgaatggcc tggggccggc ggcggaggga acgctggtag aaatgatcgc
      781 aagcgatctg accaaagaaa ggccatcggt taccgcgaac aatcttatcg ggctggccga
      841 ggggctaaca attggcgcgc caatcgtgaa gttgctggcc cgaccggccg gcattccctc
      901 ggccaccccc tcggcttcat ccgtccccac gagtgccgat ataacggcaa ctcccgcggc
      961 gaaaggtcag tttgagttaa ccgaacgggt tgtgttctgc tcggccggtg atgacgccga
     1021 tcgcattgaa atcaatatca tcggaaataa tggccagcca caagcaggcg tggcgatcac
     1081 cgtgttctgg gatgacggcc gcgactcgtt cttcaccggc ttcaccccca accaaggccc
     1141 cggatatgcc gatttcacga tgaagcaagg ggcgatctac tcggtggcca tcgcggagga
     1201 cgaagtatcg gtcgccggac ttgagccaca gctttgcgcc gacggccgca tgggtggttg
     1261 gcggctggaa taccgcgcgc gagaatcctg atcaggttcg accaaactgg cgagcgagca
     1321 gcgcggccga cagatggatg agcgtcggcc gtccgtgagg gcaggtgtgg ggcacatcgc
     1381 acgcctcgag ttgcaggatc atcgcttcca tctcttgcgt gctgagcgtt tgtccggctt
     1441 tcaccgccgc cgttttgcac acccgcttga tgaccagcgc ctccgtctcc gacttcaagg
     1501 gcgtggtctc attttccaga tcggcgacga cggcggccaa tgccgccgcc ggatcggcgc
     1561 gactcagaat ggcgggcacg gcccgcatcc gaaaggcgtt cgggccgaat gattcgatct
     1621 cgaaccccag cgaggccagc aggtctgact gctcctccac cagatgggat tgcgatggcg
     1681 acaaatagac cgtcgccgga gcgaccaatc cctgtgaagc cacgcgctgc ccctgccgct
     1741 gggccagaaa ctgttcatac aggatacgct cgtgggccgc atgctggtca atcaggtaca
     1801 gcccatcagg cccttcggcg atgatatagg cggccccaac ctggccgatc acgcgcatga
     1861 tcggcaggcg cgacgagtcg gttcccggca gcggcacagc cggtgaatct atgtcgccgt
     1921 cggcgcgctc cggctccggc ccgcgcaatt gccaggggat gtcgagctgc gtggatggca
     1981 ttccgtcggt gtcgctcccg ggtcgaaagc taccccagcc cgaaggcgag gacgacccaa
     2041 acggccagcc gctcgctccg cgaacgggcg aggtttcaac cagcgccgcg cgcaccgccc
     2101 gctgcaccgc gccgaatacc gcgccctcat cccgaaagcg cacctcaacc ttcgtgggat
     2161 gaacgttgac gtccacctct tccggcggca gctcgatgaa caggagcgcc agggggaagc
     2221 ggccgaccgg cagcagcgtg tgataggcct gcacgacggc aaaagtcagg cgcgtgtcgc
     2281 ggatggtgcg gccgttgacg aatagctgga tatgattacg gcctgaccag gtgagggccg
     2341 gatcgctgac atagccatgc acgtcgatct tgtaccgatg cccgtcgcca tgagccgctc
     2401 ctgattcagg cggcaagacc tcgatgagct gtcgcgtcgt atcggggcca tagacggcca
     2461 gcaggacatc ggccgtcgcc ccgctgcctg tcgtttgata ggtgatccgc ccgtcgtggc
     2521 tgaggcgaaa acgaatttcg ggataggcca gcgagtagcg cgtgacaaat tcatcgatta
     2581 gccgcttctc cgtggtgacg ctcttcagga atttgaggcg agccggcacg ttgtagaaca
     2641 ggttctcgac ggcgatgacc gtgcccaccg gcgcgccgac gatttcacga gccgccgtca
     2701 tgccgccctc cagcagcagg cgcacgccgg aagccgactc cctcgcccgg ctcacgacgg
     2761 tcaggcgact gacggccgag atggcggcca acgcctcgcc gcgaaagccg agcgtgcgaa
     2821 tgacccacag gtcgtcggcc gacgtgagtt tggaagtggc gtggcgatgg aatgcgacct
     2881 ctatctcgcc ggcggggatg ccgtcgccat tatcggcgac ctggatcagc tcgcggccgc
     2941 cggcgcgaat gtcgatggtg atcgccgtcg ctccggcgtc gatcgcgttc tcgacaagct
     3001 ccttaacgac ggaataaggg cgctcaacga cctctcccgc cgcaatttgc gaggccagtt
     3061 gatcgggcag gacatggata gtcatcggta aattgtacca aattctccat gccgctgcct
     3121 aagcacgggg tgagacggcc gcgcgcccga aattgcccca tggggcagga atctctatac
     3181 ttcgccctgc gaggtaaata aatgcccttt caaccaactt atgacgtaca accgggcaga
     3241 atgccctata cgctcaaacc tgccgcgacg gcctcaggac gacagacagg ggttctgatc
     3301 ctgcatggct tcatcggcag cccgctcagc tcgcggccga tggcggaata tcttaaccgg
     3361 caaggattct ttgcccattg cccgcttctg cccggtcacg gcagctaccc caacaagctc
     3421 catggcatct cctccaaagc ctggctggcc gaagtcgagg aggcgtttca gtttgcgcgg
     3481 ggagagtgcg acgacctgtt catcgtcggc cattccatgg gcaatgtgct cgcggcccat
     3541 gtggccatca agttcggcgg ggttcgcggc gtggccatgc tcgcgccggt catcgacgtc
     3601 cccgactcgc gcctgcgatg ggtgaagtac gtgcgccatt tcgtcccctg gtactacccg
     3661 cacaaaagca ggagcgagag tatgcagcac ctcgtgcgcg agcgcgtgct ggatttcgat
     3721 ccgaccatcg atttcgacag tcccgaattt caggagcagc tacccgaagt gagtcgcgtg
     3781 ccgctttccg gcatgcatgg gatggtcgag atgatcgaaa aaggccgctc gctctggccg
     3841 aagctggacg tgccggttcg cattttcgcg ggggaagatg ataatgccgc gccaccggac
     3901 aacgcgcgcc ggatctttgc cttgctgccc aacgcggaca agaaattgac catatatccc
     3961 gatgtgggcc atgaactgat gcgccccttc gaacccatcc acaagacggt ttgggagtcc
     4021 atctattctt tcatcatcga gcacacgccc ccgtccggca aaaccgaaaa taacgaggaa
     4081 taaccatgtc cgattatgtt acctttccaa agggcttcgt ctggggcgcg gcgacggccg
     4141 cctaccaaat cgaaggtgcg tggaatgagg acggcaaggg cgaatccatc tgggatcgat
     4201 tcacccacac gccgggcaat atcatcgacg gcagcaccgg cgacgtggct tgcgaccatt
     4261 accatcgctg gccggaagat atcgctctca tgaagtcgct tgggcttcag gcctatcgct
     4321 tttcgatcgc ctggtcacgg gttctgcccg acggccgcgg gaaggtcaat ccggccggcc
     4381 tcgacttcta cagccgtctg gtcgatggcc tgctggaggc cggaatcgca cccttcgtga
     4441 cgctctatca ttgggatttg ccccaggcgc tgcaggatga gggcggctgg ccggaacgaa
     4501 ccacggccga agcgttcgtt gaatatgccg acatcgtctc gcgccggctc ggcgatcggg
     4561 tcaagcgctg gatcacccac aatgagccgt ggtgcgccag catcctcagc caccagtacg
     4621 gcgaggacgc tcccggctgg cgcgacacgg ccgccgccgt ccgcgcggcc catcatctgc
     4681 tgctgtccca tggctgggcc gtgccggtca tccgcgccaa cagccccgac agcgaggtcg
     4741 gcatcacgct gaactttgag gcgatcgtgt cggcttcgga aagccccgcc gatcgacgat
     4801 tgacccgcat cgaggacggc acgtttaacc gctggtttat cagcccgctg ttcggccgtc
     4861 agtatcccgc agacgccatg gcccactacg agccatactt cccgcagggg atggagttca
     4921 tcaaacccgg cgacttcgac gccatcgccg cgccgatgga cttcctcggc gtgaactact
     4981 acatgcgccg aatcgtgcgc gacgagtctg ccgcagataa ttggccccag acgctcttcc
     5041 ccgacctgcc caaatgcgac atgttctccg gtaccgacga cccggtttac tgggaaatct
     5101 accccgaagg actatacaac ctgctaaacc gcgtctactt cgaatacaag ccgccgaaga
     5161 tttatatcac cgaaaacggc accagcttca tggatgttcc cgacaaaaac gggcgcatct
     5221 ccgattcgcg ccggatcggc tacttgcgcg atcattttgc cgccacccat cgggcgatgc
     5281 aaaacggcat ccccctgtcg gggtacttcg tctggtcgct gatggacaat ttcgagtggg
     5341 ccaagggcta tcagcagcgc ttcggcatgg tctgggtgga ttacgagacc cagcagcgca
     5401 cccccaagga cagcgccagg tggtacagcg aagtcatcgc caataacggg ttcgcgtcgg
     5461 ccgattagcc tgtcccccat caatcatgca gaccaacccc actccatcaa ccaccccgaa
     5521 caatcccggc agcaatgccg ccgtgctgcc gatcctgctt ctggcggcgg ttgtcggcgg
     5581 gcttctggcc gttatcctgc cggcggtgtg gatcgcggag cagttcgcca tcattggcgg
     5641 ggaaacgccc ggccgtggct cgtggctcgt ggctcatggg gtgttggcgc tgtcgatcct
     5701 gctgccgtcc ctcgtgttgg ccgcggtcgt caaaaccccg cgcatgcgtg ccgtattccg
     5761 gacctggtcg ctggccggtc tgtttgccct gatcatgctg cccgtccagc ttgttcccgg
     5821 cccatcggca caaacggccg cgttggtgca aatcggctgc gcggcggcat tcctgctcat
     5881 aatgaggctc atccgccgcc ggaacgcggc aatcacatcg accgccgccg gatcgttatg
     5941 gccggtcata cttcttgccg cgctcatcgg ctggccatgg gtactatggg gagcgttggg
     6001 atcgtggctg gacacgacgc tgaaccttct ggcttccgct ctgttcggtc tggccgcggc
     6061 tgcaacgctg cgccacgagc tatggtcgca tatcaacccc aaccagggaa atccgccgct
     6121 gcccttcttc atcgccggag tagcgacgag catcgcgttg atggtcatgg gcgctgcact
     6181 cgggcacaac ggccagcagc tcgtcctgtt ggccctgatg gccgggttgg gctggctggt
     6241 ggcggccgtc ggtcgccgag gtcggggcgt ggaatccgct atccggccgc aagccacgct
     6301 gctcatcggg ctggtcgtcg ccctgccgct gctcttcttt gatcctgaag agctggccct
     6361 cgtcctcaac ctgggttcac gggatgtggg ctatttcgtc ttttatgcca cgctggtcgg
     6421 cctgggcatg gctttggggc tggcgatcat cgtctggatc acgagaaaca ccggcggcgg
     6481 ccgagccatc cctgcccttc tggctactgg agcgctggcc ggcctggcag ctatttatgt
     6541 gttcgtgggg cagccgggct ggcatggcga gcggctctac gtcgtcatgg ctgatcaggc
     6601 cgatctctcg gccgcggtgg atatcgtcga tcccatagag cgccgccaat atgtctatga
     6661 gcggctcacc gaccacgcca acgccacgca ggcgggagtt cgccaggcgc ttgacgccgt
     6721 cggcgtcaac taccgcccct actacctcgt gaatgcgctg gaggtcgatg ctgggccact
     6781 cgtgcgcctg tggctggcgg cgcaacccga agtggatcgg gtgcttttaa gccccgaact
     6841 gcggccgctg cccgaacagc cacccgtgac gacgggcagc gccaccggcc ccgacgagcc
     6901 ggaatggaat ctggtcgata tcggcgcgcc acaggtgtgg caagcgtttg gcgcgcgcgg
     6961 ggcggggatc atcatcggcc agagcgattc gggcgttgat ggcgcccatc ccgaattgag
     7021 cgcgcaatat cgcggggcgc aggccgcaag cccgaccggc aacgactaca actggtatga
     7081 tccatggaac gcgtccccct cgcccaccga catcggcggc cacggcacgc acacgctggg
     7141 gtcgattctg gggcgtacgg tcggcgtggc tccggatgca agctggattg gctgtgttaa
     7201 tctggggcgc aatctgggga acccgccgcg ctatctggat tgtctccagt ttcatctagc
     7261 ccctttccct caggcgggcg atccgctccg cgacggccgg ccggaatggg gcgctcatgt
     7321 gctcaacaac tcctggggtt gcccggagat cgagggctgc gacccgactt ccctgttggc
     7381 ggcggtgcgc gcgctgcggg cggcaggcgt gttcgtcgta gcctcggccg gcaatgacgg
     7441 cagcgcctgc agcacgatag cggacccgat cagcctctac gatgaggtct tcacggtcgg
     7501 cgctatcgac gagtttggcg caatcgcgcc gttcagcagt cgcggcccgg tgctggccga
     7561 cggcagcggc cgaatcaagc ccgacatcgt cgccccgggc atgggtgtat tgtcggccat
     7621 gccgggcggg acctacgaat ataacagcgg cacctcgatg gccgggccgc atgtagccgg
     7681 ggtcgtcgcc ctcctctggt cggccgatcc ggcgctcatc ggcgacatcg atcgcaccga
     7741 gcgcatcctg gcccaaacgg ccgtcccgct cgacgagcta ccgggcctgt atgcctgcgg
     7801 caatccgggc gaaatcccca gcaatgtctc cggttatggc ctgatcaatg cctacgcggc
     7861 cgtaaagctg gctctctctg aaaacgaata gaggctgcgg ggatatgttc cgacagcctc
     7921 tatcaacgct ttctgcacac ttattgcagt gttacataaa gaagtttaat ccacaggtta
     7981 cgcgtagatt aaagaaaaat cggtgaaatc tgtgcaatct gtggatgctt ttccctgtta
     8041 gatcagctca acgatcgtcg cctcgccctg ccccaccccg acgcacaagg ttgccagccc
     8101 ataacgcatg gactcgccgg catggcggcg gcgcctcatc tcgtgcagca aggtcgccaa
     8161 gatgcgcgcc ccggagcagc ccaacggatg gcccagagcg atggctccgc cgttgacatt
     8221 ggtgatctcc ggccgcatcc ccagttcgcg catgaccgcc agcgactgga cagcaaaagc
     8281 ctcgttcaac tcgatcagat cgatgtcatc gatcgtcagc ccgacgcgag ccagcgcctt
     8341 gcgcgtggcc ggaaccgggc caagccccat cgtgcgcgga tcgacgccgg ccgcggccga
     8401 accgatccag cgagccatcg gcttcaggcc cagcgcctca gcccgcccgg ccgacatcag
     8461 cagcacggcc gccgcgccgt cgttgaggcc gctggcgttg ccggccgtca ccgtgccgcc
     8521 ttgccggaaa gcgggcttta atttggacag aacttccata cttgtgtcaa ggacgtagcc
     8581 gccctcgccc tttcggatgc ggggatgctc atcggtgtcg aagacgatgg ggtctccctt
     8641 ccgctggggg aatacgaccg gcacgatctc ctcgcggaag tagccgcgat tgatggcttc
     8701 catggcccgg cgctggctct ccagcgcgaa tgcatcctga tcctcgcgac tgatgacgcc
     8761 gccgctgatt gcgccggcca cgctctgctc atagatgttc tcggccgtct cgcccatcgc
     8821 ctccagcgga aacagcgctt gcatctttgg gttgggatag cgccacccca gggtcgtatc
     8881 ccaggcggtg acgttccccg acgggccaaa ggcgcggctg ttcttgggca gtgagtaggg
     8941 ggcacgggtc atggactcaa cgccgccggc aatgtagata tcgccctcgc cgctgcgaat
     9001 ggcccgcgcg gcctggttca cggcgttcag cccgctggcg cacagccggt tcatcgtcac
     9061 cccgccgacg gaatggggca gcccggccag aagcgccccc atgcgggcca cgttgcgatt
     9121 gtcctcgccc gcctgattgg cgcagccaaa atatacttct tcgacctcgg ccgggtcgat
     9181 g
//
LOCUS       DAOMQG010000111         9006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3321_length_9006_cov_9.682158, whole genome shotgun sequence.
ACCESSION   DAOMQG010000111 DAOMQG010000000
VERSION     DAOMQG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3321_length_9006_cov_9.682158"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..>2109
                     /locus_tag="PLC06_07880"
     CDS             199..>2109
                     /locus_tag="PLC06_07880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRO90400.1"
                     /translation="MLDQIIIEPDEHEGDLSCLELLDEAIATHEGILDVAVDTAGETV
                     SFAYDPQRVKEGEITGLALEVAPILQDRWQTCTMRLEKRGGRGCESCALNLERHVGSL
                     PGVRRATASFAGGVMAVHYDDELISPHDIVRRVEAFGVGVEPSASAVPLETAPAVVDG
                     RWSAIKEWLTPQKLQAILTVITFVAMIGGWLVERYATAAPAWLATTLFVVAYVAGGAF
                     GLKGGIESLMARTIDIDLLMILAALGAAFVGEPFEGAMLLFLFSLSNTLQDFALDRTR
                     NAIRALMELRPAQAEVYRGDRLVTLPIEQVRVGDRMLVRPGDRIALDGVVMTGSGSVD
                     QASLTGESVPVYKQKGDPCFAGTINQDGSLEIGVTKLAKDSTIARLIKMVEEAQSQKA
                     ETQRFIDRFEQYYAVGVIVLTALVAAVPVLLMGHDFNAAFYTAMTVMVAASPCAIVIS
                     TPATVLSAIGNGARRGVLFKGGIHVENAATVKVVAFDKTGTLTVGKPEVTTVRITNYE
                     LRITNEDAAAVGPDDLLRLAAAVEARSEHPLAKAVVTEAEKRGIDTPEVRHFQSVAGQ
                     GVRGSVHDPVLGKVEIHIGNPRYFASFESGGRLAAVTAVVNELEAEGQTSVIVAQAIE
                     GPAVRFLGVIGMA"
     assembly_gap    2110..2119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2120..2641
                     /locus_tag="PLC06_07885"
     CDS             <2120..2641
                     /locus_tag="PLC06_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006667197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRO90401.1"
                     /translation="AVVRELKESGVERVVMLTGDNKRVADRIAAAVGVDEAFADLMPE
                     DKVTAVRRIRDTYGPVAMVGDGVNDAPALATATIGIAMGAAGTDVALETADIVLMADD
                     LRNIPYVIRLSRATRRTLIVNLGFALTMIALMLIAIFARDLPLPLAVIGHEGGTVLVS
                     LNGLRLLGYRDKH"
     gene            complement(2725..5082)
                     /locus_tag="PLC06_07890"
     CDS             complement(2725..5082)
                     /locus_tag="PLC06_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HRO90402.1"
                     /translation="MKQRIVVYLLIAFLLAVTGTAARAQESTPMPCMDCRIPIPPIWN
                     MEGLEIPYQRVSVTIDNQVATTRVEQLFRNPNDWMLEGTYFFPLPPGAAVSELTMWVD
                     GTPIEARILAKDEARAIYDAIVRRMRDPALLEYVGADAIQANVFPIPPGDERKIEIEY
                     SYLLTAENGAFHFIYPQSAGLYTQTSLDEQSIRVEVNSDEAIRTLYSPSHRVAIERPT
                     DFSAIVGYEDANVTPTDDFELYYTVSPEEIGLTLLSYKDPGEDGFFLLLVAPGIEPGE
                     VVAKDVIVILDTSGSMEGEKMVQAKEAAAWVIDHLNPEDRFALVSFSTSVSLYEPELL
                     PANQPGNYRQHIESLQAVGGTNISGALLEAANLAAGAGDDHRPTTIIFLTDGLATEGI
                     TDTPMLLQAVDQAMPDNARVFAFGVGDDVDTILLDTLAAEQRGATTYVRPGQAVDEAV
                     SAFYAKVGSPVLTDIVLDTGDIRIDQVYPVELPDLFAGTQLVIAGRYREGGPATVTLT
                     GTANGREQTFTYEDQLFRDEGGEPFIPRLWATRAIGHLMQQIRLQGENPELVQSIVNL
                     STRYGVITPYTSFLIEEDDIIAQTGAPDLQAEAEQALSAPAEVSGAAAVDRAAVEGSL
                     SAAEAPMPMATVVVSGDRGQTVAQALVQSAGRRTFFLRSGVWVDSAYDGESVPQVIPF
                     ASDAYFNLLDNRPDLGEALALGEEIIVVIDGDAYRVSAQGDYTARPDTPQSEPQITEQ
                     ATPPASSATRPPVEAFGIQLCASAFILPLVVVAGWKASGRRKKSS"
     gene            5224..5802
                     /locus_tag="PLC06_07895"
     CDS             5224..5802
                     /locus_tag="PLC06_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90403.1"
                     /translation="MRRGLLVALLFITLLAAAACNRLEPAAPDPGLDFSDPDVGGGVG
                     IDDGPETPIPTAAPTNEASPRLDATAEALATFPPVTATPAALPVDTPAPVETPLETPP
                     PPATEATPAPSATPEEAGLIVHTVQPGETLFHIGLQYGLSWVVIAEFNGILNPDAIDV
                     GQEIRIPPTPTPTAEARSIAPIVAERSGRSVD"
     gene            5826..6875
                     /gene="thrC"
                     /locus_tag="PLC06_07900"
     CDS             5826..6875
                     /gene="thrC"
                     /locus_tag="PLC06_07900"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRO90404.1"
                     /translation="MWPGVIEAYRSYLPFGADAPVVTLNEGNTPLIHAPRLAEEIAPR
                     AGLRLYLKYEGLNPTGSFKDRGMTAAITQAVIEGAETVICASTGNTAASAAAYAARAG
                     LRCLVLVPRGKIALGKLAASLAYGAEVISIDGSFDDGLRMVREITERRPIALVNSVNP
                     WRLEGQKTGAFEIIDQLDGRVPDWHCLPVGNAGNISAYWMGYKQYGRGLPRLLGGQAA
                     GSAPIVLGRVVEKPETIATAIRIGNPARWRQAVEALDESDGIITAMDDEDILEGWRML
                     AQLEGVFVEPASATGLAALKQQIALGEIDAAGKTAVVVLTGHGLKDPGTAVELARTPI
                     VLPASLGALEDYLAS"
     gene            complement(6883..7308)
                     /locus_tag="PLC06_07905"
     CDS             complement(6883..7308)
                     /locus_tag="PLC06_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90405.1"
                     /translation="MNEKEPLKITLEDLGEEMKSEAATPERGKLADSAAELGHKVGGA
                     VMGSASRVAQKIADTTTEATSRSAEAVKDKVTETIQAQSKATADAIEARLREIDWKEE
                     AQKGAEGGLRWISKRLEELAEKLRPDKETPTDTPNEKSA"
     gene            7479..8516
                     /locus_tag="PLC06_07910"
     CDS             7479..8516
                     /locus_tag="PLC06_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006078794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90406.1"
                     /translation="MPKRSVSRVTIIATILFVGSVAAFLVTALRAEGRSTSDEGLLSI
                     FMPAVLADMGGTGVSPTPTLETTPTLTATIEATTTPTITSIATSTDVPTGTPTATLTA
                     VPTETPTATATPTETPTATAMPTMTPTEEPQATATDTSTPTQTATATLTPTPTQTATA
                     TLTPTVTPTLPPGEEMLVFDWNKPVTKAESGFAIDNPPLANGDWTTPTNFAQGTLYFR
                     AEIRNQPVAQDQMRLGFCVWQRIDKIDYENCNGQAVPGRKGTVVTWSVPVANMWKKDG
                     RSIIWSQPRSRNGFAVRNKNNLPVSDKQGWNWNGENPDDWYPLDLRFTVVVVEKGASF
                     SGWDNYITDSD"
     gene            8816..>9006
                     /locus_tag="PLC06_07915"
     CDS             8816..>9006
                     /locus_tag="PLC06_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90407.1"
                     /translation="MNTDTGVLKWARVLGLALMMAALAAFAFAAPSGLRADGENAVAN
                     GDFESGTTGWTCKSCTLSAG"
BASE COUNT         1659 a         2623 c         2963 g         1751 t
ORIGIN      
        1 tgagacgatt gcattctcga agaaccgagc taaaatttaa ccagtgttaa atttagcact
       61 aacactaatt aataatgatt tcgctttttt gtcatgatcc aaacatgtcg aagggcacta
      121 tcggcaaagg agcgcatgag agacaataaa acaagcagtg acggaagcga atgaacgcca
      181 ccgcccggaa attcggatat gttggatcaa ataatcattg aacctgacga acatgaaggc
      241 gacttgagtt gcctcgagtt gctggatgaa gctatcgcca cccatgaggg catcctcgat
      301 gtcgccgtcg atacggccgg ggagacggtg tcgttcgcct atgacccgca gcgcgtgaaa
      361 gagggtgaga tcaccggcct ggctcttgag gtcgcgccga tcctgcagga tcgctggcag
      421 acctgcacca tgcggctgga gaagcgcggc ggccgcggct gcgagtcgtg cgcgctgaat
      481 ctggagcgac atgtcggtag cctgcccggc gtgcggcggg ccacggccag cttcgccggc
      541 ggggtgatgg ccgtccatta tgacgacgaa ctcatctcac ctcatgacat tgttcgccgt
      601 gtcgaggcat tcggcgtcgg cgtcgagccg tcggcgtcgg ccgtgccgtt ggaaacagcg
      661 cccgcggtgg tcgacggtcg ttggtcggcg atcaaggaat ggctcacccc acagaaactc
      721 caagccatcc tgaccgtgat caccttcgtt gccatgatcg gcggctggct ggtggaacga
      781 tacgcgacgg ccgcgccggc atggctggcc acgaccctct tcgtggtggc ttatgtggcc
      841 gggggcgcgt tcggccttaa gggtggcatc gagtcgctca tggcgcgcac catcgatatc
      901 gacttgctaa tgatcctggc cgcgctgggg gcggccttcg tcggcgagcc gttcgagggg
      961 gcgatgctgc tcttcctctt ctcgctgtcg aacaccctgc aggattttgc tctggatcgg
     1021 acgcgcaacg cgattcgcgc cctgatggag ctgcgaccgg cgcaggccga ggtctatcgc
     1081 ggcgaccgac tggtgacatt gcccattgag caggtgcgtg tcggcgaccg gatgctggtg
     1141 cgtcctggcg atcgcatcgc cctcgatggt gtggttatga ccggcagcgg cagcgtggat
     1201 caggcgtcgc tgaccggcga atcggtgccg gtctacaagc agaaaggcga tccttgcttt
     1261 gccgggacga tcaatcagga cggcagcctg gagatcggcg tcaccaagct ggccaaagat
     1321 tcgactatcg cccggctgat caagatggtg gaagaggccc agagccagaa ggccgagacg
     1381 caacgtttca tcgaccggtt cgaacagtac tacgccgtgg gagtgatcgt cctgacggcg
     1441 ctcgtggcgg ccgtgccggt gctgttgatg ggccatgact tcaatgccgc cttctacacg
     1501 gccatgacgg tcatggtggc cgcctcgcca tgcgccatcg tcatcagtac cccggcgacg
     1561 gtgttgtcgg caatcggcaa tggggcgcgg cgcggcgtgc tgttcaaggg tggcatccat
     1621 gtggagaacg cggccacggt gaaggtcgtc gccttcgaca agacgggcac actgacggtg
     1681 ggcaagccgg aagtgacgac agtgcgaatt acgaattacg aattacgaat tacgaatgaa
     1741 gatgcggcgg ctgttggtcc cgatgatctg ctgcggctgg ccgcggctgt ggaggcgcgg
     1801 agcgaacacc cgttggcgaa ggcggtcgtg accgaggcag agaagcgagg tatcgacacc
     1861 ccggaggtgc ggcacttcca gtcggtcgcc gggcaggggg tgcgcggttc ggttcatgac
     1921 ccggtgttgg gcaaggtcga gattcatatc ggcaacccgc gctatttcgc gtcatttgag
     1981 agcggcggcc ggctggcggc ggtcacggcc gtcgtcaacg aactggaggc cgagggccag
     2041 acgagcgtca tcgtggccca ggcaatcgag gggccggcgg tgcgtttcct gggcgtcatc
     2101 ggcatggccn nnnnnnnnng cagttgtccg cgaacttaag gagtcgggcg tggagagggt
     2161 ggtgatgctg acgggcgaca acaagcgcgt ggccgaccgt atcgccgcgg cggtgggcgt
     2221 cgatgaggcg tttgccgacc tcatgccgga ggacaaggtg acggccgtgc gccgcatccg
     2281 cgacacctat ggcccggtgg cgatggtggg ggacggggtc aacgacgccc ccgcgctggc
     2341 cacggcgacc atcggcatcg ccatgggcgc ggccggcacg gacgtggcgc tggagacggc
     2401 cgacatcgtg ctcatggccg atgacttgcg taatatcccc tacgtcatcc gcctgagccg
     2461 ggccacgcgc cggacgctga tcgtcaacct gggctttgcg ctgacgatga tcgcactgat
     2521 gctcatcgcc atcttcgccc gcgacctgcc gctgccgctg gccgtcattg gtcatgaggg
     2581 cgggacggtg ctggtgtcgt tgaatgggtt acggttattg gggtataggg ataaacatta
     2641 accttgtctt ggtcctgttg aaaaaagaag agcctgcccg gattaccgga caggctcttc
     2701 ttattcatga tgatgacagg gtcgtcagct gctcttcttg cgtcgtcctg aagccttcca
     2761 acctgccaca accaccaacg gcagaataaa cgccgaggcg cataactgga taccgaacgc
     2821 ctccacgggc ggtctcgtcg cggagctggc gggaggtgtt gcttgctctg tgatctgcgg
     2881 ttcgctctgc ggtgtatcag gtcgcgcggt ataatcgcct tgtgcggaaa ctcgataggc
     2941 gtcgccgtcg atgacgacga ttatctcctc gcccaacgcc agcgcctcgc ccagatcggg
     3001 ccggttgtcg agcaggttga agtaggcgtc ggaggcgaag gggatgacct gcggcacgct
     3061 ttcgccgtcg taggcgctat cgacccatac gccgctgcgc aggaagaagg tgcgacggcc
     3121 ggcactctgc accagggcct gggccaccgt ctggccgcgg tcgcctgaga cgaccactgt
     3181 cgccatcggc atcggcgctt cggccgcgga cagcgatccc tcgaccgcgg cccggtcgac
     3241 ggccgccgcg ccgctgactt ccgcgggagc cgacaatgct tgctcggcct ccgcctgtag
     3301 gtcaggcgct ccggtttggg caatgatgtc gtcttcctcg atgagaaagc tggtgtaggg
     3361 tgtgatgacg ccgtagcggg tgctcaggtt gacgatgctt tgcaccagtt cggggttttc
     3421 gccctgcaag cggatttgct gcatcaggtg gccgatggct cgcgtggccc acaggcgggg
     3481 gatgaacggc tcgccgcctt cgtcgcggaa cagttggtct tcataggtga aggtctgctc
     3541 gcggccgttg gcggtgccgg tcagggtgac ggttgccggg ccgccctcgc gatagcgccc
     3601 ggctatgacg agctgcgtcc cggcgaacag gtcaggcagc tcgacggggt agacctggtc
     3661 gatgcggata tcgccggtgt cgagcacgat gtcggtcagc acgggcgagc cgactttggc
     3721 atagaaggcg ctgaccgcct catcgacggc ttgccccggc cggacgtagg tcgtcgcgcc
     3781 gcgctgctcg gccgccaggg tgtcgagcag gatggtgtcg acgtcgtcgc ccactccgaa
     3841 ggcgaagacg cgtgcgttgt cgggcatggc ttggtcgacg gcctgcagca gcatgggggt
     3901 atcggtgatc ccttcagtcg ccagcccgtc agtcaggaag atgatcgtcg tggggcgatg
     3961 atcatcgcct gcgccggcgg cgaggttggc cgcctccagc agcgcgccgc tgatgttggt
     4021 gccgccaaca gcctggaggc tctcaatatg ctggcggtag ttgccgggct gattggcggg
     4081 cagcagttca ggctcgtaga ggctgacgct ggtgctgaag ctgaccaggg cgaaacgatc
     4141 ttcggggttc aggtggtcga tgacccaggc ggccgcttcc ttggcctgga ccatcttttc
     4201 gccttccatg ctgccgctgg tgtcgaggat gacgatgaca tccttggcca ccacctcgcc
     4261 cggctcgatg cccggtgcga ccagcagcag gaagaacccg tcctcgccgg ggtctttata
     4321 gctgagcagc gtcagaccaa tctcttccgg cgacacggtg tagtacagct cgaaatcgtc
     4381 ggtaggggtg acgttggcgt cttcgtatcc cacgatggcg ctgaaatcgg tcggccgttc
     4441 gatggccaca cggtggctgg gcgagtagag ggtgcggatc gcttcatcgg agttcacctc
     4501 gacgcgaatg gattgctcgt cgagcgacgt ttgggtgtag aggccggccg attgcggata
     4561 gatgaagtgg aatgcgccgt tctcggccgt cagcaaatag ctatactcga tctcgatctt
     4621 gcgctcgtcg ccgggcggga tggggaagac gttggcctgg atggcgtccg cgcccacgta
     4681 ttccagcagg gccggatcgc gcatccggcg gacgatggcg tcatagatgg cgcgggcttc
     4741 gtctttggcc agtatcctgg cttcgatggg cgtgccgtcg acccacatgg tcagctccga
     4801 cacggccgcg ccgggcggca gggggaagaa gtaggtgcct tccagcatcc agtcgttggg
     4861 gttgcggaag agttgttcga cgcgggtcgt ggcaacctgg ttgtcgatag tcacgctcac
     4921 gcgttgataa ggtatttcca gcccctccat attccagatc ggcgggattg ggatacggca
     4981 gtccatgcag ggcatgggtg tggattcctg ggcgcgggca gcagttcccg tcacggccag
     5041 cagaaaagcg atcaatagat aaacaacaat cctttgcttc atctcgttcc tccatcaagg
     5101 tatgcctgat tgagagacgc ggatgcgtgg aggaatgttc cggcaggtgg gtgatctttg
     5161 gctctaccgt cgggcaaatc cgtgctatca ttcctcggat tgaccggagg tatttgattg
     5221 acgatgcgtc ggggtttgtt ggtcgctctg ctcttcatca cgctattggc cgcagccgct
     5281 tgtaaccggc tggagccggc cgcgcccgat ccggggctgg atttcagcga tcccgacgtc
     5341 ggtggtggcg taggcatcga cgatggcccg gagacgccga ttccgacggc cgcacccacc
     5401 aatgaagcct caccgcgact tgatgcgacg gccgaggcac tggcgacctt cccgccggtc
     5461 acggcgacgc ctgcggcttt gccggtagat acacctgccc cggtggagac gccattggag
     5521 acgccaccac cgccggcaac cgaagcgaca cccgcgccct ccgccacgcc ggaagaagcc
     5581 gggttgatcg tccataccgt tcagcctggc gaaaccctgt tccatatcgg cctgcaatac
     5641 ggcctctctt gggtagtcat cgccgagttc aacggtatcc tcaaccccga tgccattgac
     5701 gtggggcagg agattcgcat tccccccacg ccgacgccaa cggccgaagc gcgatcgatc
     5761 gcaccgatcg tagccgagag atcgggccga tctgttgatt gaattaataa ggagataccc
     5821 cacgtatgtg gcctggagtc attgaagctt atcgatccta cctgcctttc ggcgccgatg
     5881 ccccggtcgt cacgctgaac gagggcaata caccgctgat ccacgcgccg cgcctggccg
     5941 aggagatcgc cccccgcgcg gggttgcggt tatatctgaa gtatgagggg ttgaacccga
     6001 ccggttcgtt caaggatcgg gggatgaccg cggccatcac tcaggcggtg atcgaagggg
     6061 ccgagacggt catctgcgcc agcacaggca atacggccgc cagcgctgcc gcctatgccg
     6121 cgcgggccgg attgcgctgc ctggttctgg tgccgcgggg gaagatcgcg ctgggcaagc
     6181 tggccgccag cctggcctat ggcgcggagg tgatcagtat tgacggctcg ttcgacgatg
     6241 ggctgcgcat ggtgcgcgag atcaccgaac ggcggccgat tgctctggtg aattcagtca
     6301 acccgtggcg actggaagga cagaagaccg gcgcgttcga gatcatcgat caactcgacg
     6361 gtcgcgtgcc ggactggcac tgcctgccgg tcggcaacgc gggcaacatc agtgcctact
     6421 ggatgggcta caagcagtat ggcagaggac tacctcgcct attgggcggg caggcagcgg
     6481 ggtcggcgcc catcgtcctt ggccgcgtgg tggagaagcc ggagacgatc gccacggcga
     6541 tccgcatcgg caatccggct cgctggcggc aggcggtcga ggcacttgat gaatcggacg
     6601 gcatcatcac cgcgatggac gatgaggata tcctggaagg atggcgcatg ctggcccagc
     6661 ttgagggcgt gtttgtggag cctgcctcgg ctaccgggtt ggcggcgctg aagcagcaga
     6721 tcgcgctggg agagattgac gccgcaggca aaacggccgt cgttgtcctg accggtcatg
     6781 ggctgaagga tcccggcacg gccgtggaac tggcgcgaac gccgatcgtt ctgccggcga
     6841 gccttggcgc gcttgaggat tatctggcgt cgtgatcggg gattaggccg atttttcgtt
     6901 gggggtatcg gttggcgtct ctttgtcggg gcgcagcttc tcggccagct cctccagccg
     6961 cttgctgatc cagcgcaggc caccttcggc ccctttttgg gcttcctctt tccagtcgat
     7021 ctcgcgcagg cgggcttcga tggcgtcggc tgtggctttc gattgggcct gaatcgtctc
     7081 ggtcaccttg tccttgacgg cttcggcgct acgactggtg gcttcggtgg tggtgtcggc
     7141 tattttctgg gcaacccggg aagcggaacc catgacggct ccaccgactt tgtggccgag
     7201 ttcggctgcg ctatcggcca gcttgccgcg ctcgggcgtc gccgcttcgg acttcatctc
     7261 ttcgccaaga tcctccagcg taatcttgag cggttctttt tcattcatag gccaatcttc
     7321 ctgcacaggt atctgctctc tatacgactg gaatggggtc gagttgcaag ccgattcatg
     7381 agaagaaacc aaacacgtgg atttggggcg gggaaaatcg ttataatgtt tcggcccgca
     7441 ggcgggcgga taaacaaata aaagggaatg ggatgtccat gccgaaacgg tcagtttcac
     7501 gagttacgat tattgcgacg attctgtttg tgggaagtgt ggcggccttt ctggtcacgg
     7561 cccttcgggc ggaagggcgc tcgacgtccg acgagggctt attgagcatc ttcatgcctg
     7621 ccgtcctggc cgatatgggt ggaacgggcg tttctccgac gcctactctt gagacaactc
     7681 ccactctgac ggctaccatc gaagccacca cgacaccgac gatcacgagc atcgcgactt
     7741 caaccgatgt gccgacgggg acaccaaccg cgaccctgac ggcggtaccc acggagacgc
     7801 cgactgccac ggccacgccg accgaaacgc cgaccgccac ggccatgccg actatgacgc
     7861 cgaccgagga accccaggca acggcaaccg atacatccac gccgacccaa acggccaccg
     7921 ccacattgac accgacgccg acacaaacag ccaccgcgac gttgacgccg acggtcactc
     7981 cgacactacc gccgggcgag gaaatgctgg tgttcgactg gaacaagccg gtgaccaagg
     8041 cggaaagcgg ctttgccatt gataacccgc cactggccaa cggcgattgg accacgccga
     8101 ccaacttcgc tcagggcacg ctctacttcc gcgccgagat tcgcaaccag cccgtcgccc
     8161 aggatcagat gcgccttggt ttctgtgttt ggcaacggat cgacaagatc gattacgaga
     8221 actgcaatgg acaggccgtg cccgggcgaa agggaaccgt ggtcacgtgg tcggtccctg
     8281 tggccaatat gtggaagaaa gatggccgat cgatcatttg gagccaaccg cgtagccgca
     8341 acggttttgc cgttcgcaac aaaaacaatt taccggtcag cgacaagcag ggctggaact
     8401 ggaacggcga gaacccggat gactggtacc cgcttgatct tcgttttact gtggtcgtcg
     8461 tggaaaaagg ggcgagtttt tccggctggg acaattacat taccgattcc gactaaccaa
     8521 cctgaccaac gtactacctg gtcatgtgaa taaatcgcga atctcaccca ataatgacgc
     8581 ataattcaca gcagggacat gctacagtaa gtaaacttgc aagctacaga tacgataatc
     8641 ctcaacggaa ggactggcgt ccctgttctt ccatatcctg gaccgattgc ggtcatgggg
     8701 cgcgagagac gcagccggct gccttccggg caaattgact ggcccggctg cagacagggt
     8761 tcatatccct gacctttaga tccaaatgcg ggaaatttac atgacaggag accgtatgaa
     8821 tacagacaca ggcgtattaa agtgggcgcg cgtgctgggg ctggccttga tgatggctgc
     8881 attggccgcg ttcgccttcg ccgctccatc ggggttgcgc gccgacggcg aaaacgcggt
     8941 tgccaacgga gatttcgaga gcggcacgac cggctggacg tgcaagagct gcacgctgag
     9001 cgcggg
//
LOCUS       DAOMQG010000112         8928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3356_length_8928_cov_6.073932, whole genome shotgun sequence.
ACCESSION   DAOMQG010000112 DAOMQG010000000
VERSION     DAOMQG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3356_length_8928_cov_6.073932"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1038
                     /locus_tag="PLC06_07920"
     CDS             43..1038
                     /locus_tag="PLC06_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRO90408.1"
                     /translation="MDLTLTQEGLHAIDELLVATVSSEVELLQAASRHIIGSGGKHLR
                     PHVALLSYLAAGGTELSDAVSMAAAIEMVHTATLVHDDINDHALLRRGRPSVHARWGR
                     TFALLTGDFMFAKVYELMAPYGPTYNVIMARACSELVEGETLQAVSAKAGTIDRETYK
                     RIVALKTASLFEGAARMGALLGGGDERTVEALAVYAYNLGIAFQMVDDILDVVGDPTA
                     LGKPVGADLAQGRGAFAPNGKSILSLQGSGDVAAEAAAVAEAVAVAEVVADPDEADPI
                     ARMMAKLRTSGAIEAARLQALEMIERARVALDDVPPSPARDELLSLTDDVLNRDH"
     gene            complement(1032..1457)
                     /locus_tag="PLC06_07925"
     CDS             complement(1032..1457)
                     /locus_tag="PLC06_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRO90409.1"
                     /translation="MSELPEKLQEIVDDFALLEGNEKLEYLMELGERLPPLPPRLAAD
                     RDRMELVHECMSTVYVSAGKDADGRLTFFIDAPPESPTVRGYATILTEGLNGVTTAEL
                     LAVPNEFYLQMGLQQVLSGQRLNGIGGILAHMKRVAADQ"
     assembly_gap    1598..1697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1698..1867
                     /locus_tag="PLC06_07930"
     CDS             <1698..1867
                     /locus_tag="PLC06_07930"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HRO90410.1"
                     /translation="FTEREIVETVDFNDDTLLDLDREGNVVALTLEHARANVDLTDIS
                     FQQIAMVETVL"
     gene            complement(1930..5664)
                     /locus_tag="PLC06_07935"
     CDS             complement(1930..5664)
                     /locus_tag="PLC06_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HRO90411.1"
                     /translation="MTELTTLPAPEDDAPDDFLSRPLIAALNIDWEKAIYLTFMILAI
                     VTRFYMLGDRVVSHDESLHTQYSYQFYNGDGYVHTPLMHGPTLFHATALSYWLFGDSD
                     FASRIPDAILGVLLVLMPWFLRGWLGRRGALFASFMLLISPYVSYYSRYIRHDVYLIV
                     FAMIVFIATWSYIRERRTRYLWWFTIGLVLMVATMEASFIYIAIFGSFLVVRLLALVS
                     GAPWLREVLPRLRTPLLVALIGVVLVGAGLAMHRFMGADAAADATATTTSEGFAADPN
                     ATTAATAEATSPTDLLSRWIEVAGIGLFSLGLFLIVRGMRPHIDGYPEFDIIMLYVTL
                     ILPMASPILIRVAGWNPVDYTLRTCVLDGQETMSAVSLWAGRLTNSTCVSAFAGSPVT
                     HSTIVFLVMVAISVLVGLWWDRKRWPGFALVFTAIFLFFFTSIFTNLAGWRTGAIGSL
                     GYWLEQHGVQRGSQPGYYYFFVTTLYEFLPIIFSLAGIHLWTKQRRINGVVWFWVLAG
                     LVAMLGYSLANYFFNRSLIDPIDQSRVPGLIAAAVVLLIAAAWWFLVRLGQVKAGLEL
                     EHGLRELVDLPAFVGFVPSIIWWALLAWPAYSIAGEKMPWLSIHITIPMILLGGWYLG
                     QRVESVDRQSLFSRNGLIAIGMTALLIVMAAIALGPLLLGQIKLGSQEVDQLKNLGRL
                     LGGLLVAGGVAWLWWRYYGPLERRLRGVVVTLALFAVLAGLTIRFSYMANFVNYDYTN
                     EFMVYAHGAPATKSVVLRQLEELSMRLHGDKSIKVAFDSDVSWPFTWYLREYPNRVYF
                     GDNASQSLTESPVVIVGSKNWGNVDPFLSNNYTYHEYTFLWWPMEDYRRFSWNAVFGN
                     PADEPRRGLGNSGVRQALWDIFFYRDFQKYGEVFGGTFTAGEWPLRHNLRLYIRDDVM
                     ADLWDFGVGAVAAGPIVDPYEANQLALSPSLVINPAGVGGAAEGQLTSPRNVAIGPDG
                     LVYVADSGNHRIQVFDRDGRFVRGWGSSGTEPGQFNEPWGLVTDDEFLYVADTWNHRI
                     QKFTLDGALVAVFGQSGSPAADDPTGGLGLFFGPRDIVLIGDDRLLVTDTGNHRLQLL
                     TRDGQFLSSIGGFGNQLGQFNEPVGLAVAPDGTVYVADTWNGRIQRLTPELVPAGEWH
                     VEAWAGQSINNKPYLAADSGGRVYVTDPEGYRVLVFSGSGGYLNRFGQFGTDANSLGL
                     PNGITVGPDDTLWVADAGNHRVLAFPPIYGAAPVPSGDGE"
     gene            complement(5765..6349)
                     /locus_tag="PLC06_07940"
     CDS             complement(5765..6349)
                     /locus_tag="PLC06_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRO90412.1"
                     /translation="MDCEHTHSLIADYALGLLSPEERRRVERHIGQCPDCRAAVRRER
                     ALAPLVRGTVHRAARPAPGRLAALRPAIALPHARVVAPLYRRLAPLTVVTCLLALGLL
                     FGRGSALFAPVAYAEGTPTLGETDLQLPTATIAAVVAPAMAAGPSATGRSATRRSLQA
                     IGFDATAAPDPRTATITSASVAPTPAPLAGTVDH"
     gene            complement(6389..6994)
                     /locus_tag="PLC06_07945"
     CDS             complement(6389..6994)
                     /locus_tag="PLC06_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRO90413.1"
                     /translation="MNESTLLNEIDFPLDGSAADDAIALIGRCLSGEQSAYVELYDLY
                     SGQLYRLVYSMLGDREDAEEVLQDSFEYAFRRLDHYDARKSAFRTWLYRIAVSRCRNK
                     RRRKWLPSFSLNAFDGNEPKDPAAAPPDETAVLSERQRAVWAAVSALSPKLREVAILR
                     YYGGLQYAEIGAVLDIPPKTAESRMRLAHKTLREQLSEVAD"
     gene            complement(6991..8769)
                     /locus_tag="PLC06_07950"
     CDS             complement(6991..8769)
                     /locus_tag="PLC06_07950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90414.1"
                     /translation="MTNIEIDQTNDPTPTIRGHSSLVISSDDRSRRRTGVRSSLVTLS
                     DGLMLPIAILAAWLRFGDLARLPLSAAEATAALANWQFWSAAPLDVAVSSPAYFAFTH
                     LVMSLGASGDVAARFVPAVFGLLTVLLVWLMRGRVRPVAWLGAGLFLAVSPLLVAVSR
                     TAGGDAIALFALLLIAVTWNGRDRRSTVLAGAGLGLGLASAPLFYTGLVGLVIAGLAG
                     GEERNLSRLRYMAIAAAITFAAVSTSMLLFPAGLGAALRIFPTWLGQFGLPTDASAAV
                     SPLLALLRYDPAVYVLGVPAAVWCLRVRNRGRGPAVWLAVTAALVVLQSGTMVNVTAA
                     ILPACLLIGLLAAEIARQADPTPVIGRHTTLYTAVAILGLGALLLVATGRLARLNLLS
                     GENAMLIALSMLAFVLAGAAVLLAMAWESPAARRGLLAGAVGLLLFWHWGTAWQLSRL
                     GANDPRERWVQTGTDDDVSLLIPLLQDISRQLVNSPRDLTIAGQVESPVLRWYLRDFA
                     GFEYGPALPINTSADVIITPIDAEPSLSNDYFGADFGLERREVPNVGPVMVSDALKWW
                     LFRESTAATDDRRIVLWVRSDLARGE"
BASE COUNT         1613 a         2880 c         3006 g         1329 t
ORIGIN      
        1 acaactttca ataaaatttg aaagttgtga actgaggaag gcatggattt aacgttaacg
       61 caagaagggc tgcacgcgat cgacgagctg ctggtcgcca cggtcagcag cgaagtggag
      121 ctattgcaag cggccagccg ccacatcatc gggtcgggcg gcaaacatct gcggccgcat
      181 gtggcgctgc tctcctatct ggcggcgggc ggcaccgagc tgtccgatgc ggtgtcgatg
      241 gccgcggcca tcgagatggt gcatacggcc acgctcgtcc acgacgacat caacgaccac
      301 gccctgctgc ggcgcgggcg gccgtcggtc catgcccgct gggggcgcac cttcgccctg
      361 ctgaccggcg acttcatgtt cgccaaggtc tatgaattga tggcccccta tggccccacg
      421 tataacgtca tcatggcccg cgcctgctcc gagctggtgg aaggggagac gctgcaagcc
      481 gtgagcgcca aggcggggac catcgaccgc gagacctaca agcgcatcgt cgccctgaag
      541 acggccagcc tgttcgaggg cgcggcgcgc atgggcgcgc tgttgggcgg cggcgacgag
      601 cgcacggtcg aggcgctggc ggtatacgcc tataatttgg gcatcgcctt ccagatggtc
      661 gacgacatcc tcgacgtggt gggtgacccg acggcgctgg gcaagcccgt gggcgcggat
      721 ttggcccagg ggcgcggcgc atttgccccc aacggcaaga gcattctgtc gctgcagggg
      781 agcggtgatg tggcggcaga ggcggcggcc gtcgccgaag cggtggctgt ggccgaggtg
      841 gtggccgacc ccgacgaggc cgaccccatc gcccgcatga tggccaagct gcgcacgtcc
      901 ggcgcaattg aggccgcccg gctacaggcg ctggagatga tcgaacgggc gcgggttgcg
      961 ctcgacgacg tgcccccctc ccccgcccgc gacgagctgt tgtcgctgac cgatgatgtg
     1021 ctgaatcggg atcactgatc ggccgccacg cgcttcatgt gggccagtat cccgccgatg
     1081 ccgttcagcc gctggccgga cagcacctgt tgcagcccca tttgcagata gaactcattg
     1141 ggcacggcca atagttcggc cgtcgtcacg ccgtttagcc cctcggtcag gatggtggca
     1201 tagccgcgca ccgtcggcga ttccggcggc gcgtcgatga agaaggtcag gcggccgtct
     1261 gcgtccttcc cggcgctcac atagacggtc gacatacact catgcaccag ctccatccgg
     1321 tcgcggtcgg ccgccagccg cggcggcaag ggcggcagcc gctcgcccag ctccatcagg
     1381 tattccagct tctcattgcc ctccagcagg gcgaaatcgt caacgatttc ctgcagcttc
     1441 tccggtaact cactcatgga cgggatttta accacagatg acccggatgg cacagattga
     1501 ctgcctggtc cgctgttgaa atcctggcca catccttctt aactcacatc tcgtataatg
     1561 tggtaatatc ccgctatgtc ccgattcgcg acaacggnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnncct tcaccgagcg cgaaatcgtt gagacggttg atttcaacga
     1741 tgacaccctg ctcgacctcg accgcgaggg caatgtcgtc gcccttacgc tggagcacgc
     1801 ccgcgccaac gtcgacctga ccgacatctc tttccagcag attgcgatgg ttgagacggt
     1861 attataataa taagcggggc ttatttcgat aagccccgct tttgtcttta atcgggagag
     1921 cctgcggatt tattccccat cgcccgacgg gacgggcgcc gcgccgtaga tgggcgggaa
     1981 ggccagcacg cggtggttgc ccgcgtcggc cacccatagc gtgtcgtccg ggccgacggt
     2041 gatgccgttg ggcaggccca ggctgttggc gtcggtgccg aactggccga agcggttgag
     2101 atagccgccg ctgccgctga agaccagcac gcgataccct tcggggtcgg tcacatagac
     2161 ccgcccgccg ctgtcggcgg ccagataggg tttgttgttg atcgactggc ccgcccacgc
     2221 ctcgacatgc cactccccgg ccggaaccag ctccggcgtc aggcgctgga tgcggccgtt
     2281 ccaggtgtcg gcgacgtaga ccgtgccgtc gggagcaacg gccaggccaa ccggctcgtt
     2341 gaactgcccc aactggttgc caaacccgcc gatggagctg aggaactgac cgtcgcgggt
     2401 cagcagctgc agccggtggt tgccggtgtc ggtgacgagc aggcggtcgt cgccgattag
     2461 cacgatgtcg cgcgggccga agaacaggcc caggccgccg gtcgggtcgt cggccgccgg
     2521 ggagccgctc tgcccgaaca ccgccaccag cgcgccgtcg agcgtgaact tctggatgcg
     2581 gtgattccac gtgtcggcca cgtagaggaa ctcgtcgtcg gtgactagcc cccacggctc
     2641 gttgaactgc cccggctcgg tgccggagct gccccagccg cgcacgaagc ggccgtcacg
     2701 gtcgaacacc tggatgcggt gattgccgga gtcggccacg tagaccagcc cgtcggggcc
     2761 gatggccacg ttgcgcgggc tggtcaattg cccttcggcc gcgccgccca cgcccgccgg
     2821 gttgatgacc agcgacggcg acagcgccag ctggttggcc tcatacggat cgacgattgg
     2881 cccggcagcc accgcgccca cgccgaaatc ccacaggtcg gccatcacgt cgtcgcgaat
     2941 gtagaggcgc agattgtggc gcagcggcca ctcgccggcg gtgaatgtgc cgccgaacac
     3001 ctcgccgtac ttctggaagt cgcgatagaa gaagatatcc cacagcgcct ggcgcacgcc
     3061 ggagttgccc agcccgcgcc gcggctcgtc ggccggattg ccgaagacgg cgttccacga
     3121 gaagcggcga taatcttcca tcggccacca caggaaggtg tactcgtggt aggtgtagtt
     3181 gttggacagg aacggatcga cgttgcccca gttcttgctg ccgacgatga ccaccggcga
     3241 ctcggtcagg ctttgcgagg cgttgtcgcc gaagtagaca cggttgggat attcgcgcag
     3301 gtaccaggtg aacggccacg atacgtcgct gtcgaaggcc accttgatgc tcttgtcgcc
     3361 gtggagccgc atggacagct cctccagctg ccgcagcacg acgctcttgg tggccggcgc
     3421 gccgtgggcg tagaccatga actcgttggt gtagtcgtag ttgacgaagt tggccatgta
     3481 gctgaagcgg atggtcagcc cggccagcac ggcgaacagg gccagcgtga cgaccacgcc
     3541 gcgcagccgt cgctccagcg gcccgtaata acgccaccac agccaggcca cgccaccggc
     3601 caccagcagc ccgcccagca agcggccgag gttcttcaac tggtcgactt cctggctgcc
     3661 cagcttgatc tgccccagca gcagcggccc cagcgcgatg gcggccatga cgatcagcag
     3721 ggcggtcatg ccgatggcga tcaggccatt gcgcgagaaa agcgactgcc gatccaccga
     3781 ctcgacccgc tgccccagat accagccgcc gagcaggatc atcgggatgg tgatgtggat
     3841 actgagccag ggcatttttt ccccggcgat gctgtaggcg ggccaggcca gcagcgccca
     3901 ccagatgatc gacggcacga agccgacgaa cgccggcaga tcgaccagct cgcgcagccc
     3961 atgctccagc tccagcccgg ccttgacctg gcccagccgg acgaggaacc accaggcggc
     4021 ggcgatcagc agcacgacgg cggcggcgat cagccccggc acgcggctct ggtcgatggg
     4081 gtcgatcagg ctgcgattga agaagtagtt ggccaggctg tagcccagca tggccaccag
     4141 cccggccagc acccagaacc agacgacgcc gttgatgcgc cgctgcttcg tccacagatg
     4201 gatgcccgcc agcgagaaga tgatgggcag aaactcgtag agcgtggtga cgaagaagta
     4261 gtagtagccc ggctgactgc cgcgctgcac gccgtgctgc tccagccagt agccgagcga
     4321 gccgatggcc ccggtgcgcc agccggcgag gttggtgaag atggacgtga agaagaacag
     4381 gaagatggcc gtgaagacga gggcgaaccc cggccagcgt ttgcgatccc accacagccc
     4441 gacgaggacg ctgatggcca ccatgaccag aaagacgatt gtcgaatggg tgacgggcga
     4501 cccggcgaag gcgctgacgc aggtggaatt ggtcaggcgg ccggcccaga gcgacacggc
     4561 cgacatcgtc tcctgcccat caagcacgca ggtcctgagc gtgtagtcga ccgggttcca
     4621 gccggccacg cggatcagaa tgggcgaggc catcggcagg atgagggtca catagagcat
     4681 gatgatgtcg aattcggggt aaccgtcgat gtggggccgc atcccgcgca cgatgaggaa
     4741 caggcccagc gagaacaggc caataccggc gacctcgatc cagcggctca gcaggtcggt
     4801 ggggctggtg gcttcggccg tcgcggccgt ggtggcgttg gggtcggcgg cgaatccttc
     4861 gctggtggtc gtggccgttg cgtccgcggc cgcgtcggcg cccatgaacc ggtgcatggc
     4921 cagacccgcg cccaccagca ccacgccgat cagggccacc agcagcggcg tgcgcagccg
     4981 cggcagcacc tcgcgcagcc acggcgcacc gctgacaagg gccagcaggc gcacgacgag
     5041 gaagctgccg aagatggcga tgtagatgaa cgacgcttcc atcgtggcca ccatcagcac
     5101 caggccgatg gtgaaccacc acagatagcg cgtgcggcgc tcgcggatat agctccaggt
     5161 ggcgatgaag acgatcatcg cgaagacgat cagatagacg tcgtggcgaa tgtagcgcga
     5221 atagtagctg acgtaggggg agatgagcag catgaacgag gcgaacagcg ccccgcggcg
     5281 gccgagccag ccgcgcagga accagggcat cagcaccagc agcacgccca ggatggcgtc
     5341 ggggatgcgc gaggcgaagt cgctgtcgcc gaacagccag taggacaggg cggtggcgtg
     5401 gaagagcgtc ggcccgtgca tcagcggcgt gtggacgtag ccgtcgccgt tgtagaactg
     5461 gtaggaatat tgcgtgtgca ggctctcgtc atggctgacc acgcgatcgc ccagcatgta
     5521 gaagcgggtg acgatggcga ggatcatgaa cgtcagatag atcgcctttt cccagtcgat
     5581 attgagcgcg gcaatgagcg gccgcgacag gaaatcgtcg ggggcgtcgt cttcgggggc
     5641 aggcaaggta gtcagctcag tcatggcaag ttccgtgtgt caagacctga caggtgggcg
     5701 atgaacgcta ctcatgaagg aaggagtttt gtcgcccacc tgtcaggtct ggagatttct
     5761 tctctcaatg atccaccgtt ccggcgagcg gggcgggggt gggcgcgaca gacgcgctcg
     5821 tgatggtggc cgttcgcggg tcgggcgcgg ccgtcgcgtc gaagccaatc gcttgcagcg
     5881 accgtctggt cgccgaccgc ccggtcgccg acgggccggc ggccatggcc ggggcgacga
     5941 cggcggcgat ggtggccgtc ggcaattgca ggtcggtttc gcccagcgtc ggcgtgccct
     6001 cggcataggc aaccggagcg aagagggccg atccgcggcc gaacagcagc cccagcgcca
     6061 gcaggcaggt gacgaccgtc agcggggcca gccgccggta gagcggggcg acgacgcggg
     6121 cgtggggcag agcgatggcc gggcgcaggg cggccaaccg gccgggcgcg gggcgcgccg
     6181 cccgatggac ggttccgcgc accagcggag ccagcgcgcg ctcccgccgg acggccgcgc
     6241 gacaatcggg gcactgcccg atgtggcgct ccacgcggcg gcgctcctcg ggcgacagca
     6301 gccccagggc gtaatcggcg atcagcgaat gtgtgtgttc acaatccatt agcataaacc
     6361 caatatacac acccaccgcg ctacagcctc aatcggccac ctcgctcagc tgctcgcgca
     6421 gggtcttgtg ggccagccgc atgcgcgatt cggccgtctt gggcgggatg tcgagcaccg
     6481 cgccgatctc ggcatattgc aggccgccgt agtagcgcag gatggccacc tcgcgcagct
     6541 tgggcgacag ggccgacacg gcggcccaca cggcccgctg ccgctcgctc agcacggccg
     6601 tttcgtcggg cggcgcggcg gcggggtctt tcggttcgtt gccgtcgaag gcgttgagcg
     6661 agaaggacgg cagccatttg cggcggcgct tgttgcggca gcggctgacg gcgatgcgat
     6721 agagccaggt cctgaaggcc gatttgcgcg cgtcgtaatg atccagccgc cggaaggcgt
     6781 actcgaagct gtcctgcagc acctcttcgg cgtcctcgcg gtcgcccagc atgctgtaga
     6841 cgagtcggta gagctggccg ctgtagagat cgtagagttc gacgtaggcg ctctgctcgc
     6901 ccgacaggca gcggccgatg agcgcaatgg cgtcgtcggc cgcgcttccg tcaagcggga
     6961 aatcgatctc gttcagcaag gttgactcgt tcattcgccc cttgccagat cgctgcgcac
     7021 ccaaagcacg atacgccggt cgtcggtggc cgccgtcgat tcgcggaaca accaccattt
     7081 cagcgcgtcg gacaccatca ccggccccac gttgggcact tcgcgccgct ccagcccgaa
     7141 atcggcgccg aaatagtcgt tggacaggga cggctcggcg tcgatagggg tgatgatcac
     7201 gtcggccgac gtgttgatcg gcagcgccgg gccgtattca aatccggcga aatcgcgcaa
     7261 ataccagcgc agcaccggcg attcgacctg accggcgatg gtcaggtcgc gcggcgaatt
     7321 gaccagctgc cgcgagatgt cctgcagcag ggggatgagc aggctcacgt cgtcgtcggt
     7381 gccggtctgc acccagcgct cgcgggggtc attggccccc agacggctga gctgccacgc
     7441 cgtgccccag tgccagaaga ggagcagccc caccgcgccg gccagcaggc cgcgccgggc
     7501 cgccgggctt tcccaggcca tggccagcag cacggccgcc ccggccagca cgaaggccag
     7561 catcgacagg gcgatgagca tggcgttttc gccgctcagc aggttgaggc gagccaatcg
     7621 tcccgtggcg accagcagca gcgcccccag gccgaggatg gcgacggcag tgtagagggt
     7681 cgtgtgccgg ccgatgaccg gggtcgggtc agcttgtcgg gcgatctcgg ccgccagcag
     7741 cccaatcagc aggcaggcgg gcaggatggc ggcggtgacg ttgaccatcg tccccgattg
     7801 caggacgacc agcgccgccg tcacggccag ccagacggcc gggccgcggc cgcgattgcg
     7861 cacccgcagg caccagacgg cggcaggcac gcccagcaca taaaccgccg gatcatagcg
     7921 cagcagcgcc aacagcggcg agacggccgc gctcgcgtcg gtgggcaggc cgaactgccc
     7981 cagccacgtc gggaatatcc gcagcgccgc ccccagcccg gccgggaaca gcagcatgct
     8041 ggtggagacg gcggcaaatg taatcgcggc ggcgatggcc atatagcgca acctggacag
     8101 gttgcgttct tctccccccg ccaaccccgc gatcaccagc cccaccagcc ccgtataaaa
     8161 cagcggcgcg ctcgccagcc ccagccccag cccggccccg gccaggacgg tcgagcgacg
     8221 gtcgcggccg ttccacgtca cggcgatcag cagcagcgca aacagcgcga tggcgtcgcc
     8281 gccggccgtg cgcgacacgg ccaccagcag cggcgacacg gccaggaaca gccccgcccc
     8341 cagccacgcc accggccgca cgcggccgcg catgagccag acgagcagca cggtcagcag
     8401 cccgaacacg gcgggcacga agcgcgccgc cacgtcgccg ctcgcgccga gggacatgac
     8461 cagatgggtg aaggcgaagt aggccgggct gctcacggcc acgtcgagcg gcgcggccga
     8521 ccagaactgc cagttggcca gcgccgccgt ggcctcggcc gccgagaggg gcagccgcgc
     8581 cagatcgccg aagcgcagcc acgcggccag gatggcgatg gggagcatca ggccatcaga
     8641 tagagtgacg agtgacgaac ggacccctgt ccgccgacga ctcctgtcgt ccgacgaaat
     8701 gacgagtgaa gaatggcccc gtatggtcgg ggtcgggtca ttcgtttggt cgatctctat
     8761 gttggtcaag ctgtaatctc catccctttt ccttacggga tctctttggt tcgtagttag
     8821 cagctttagc tgcgttcccc ggagcggcgc taaagcgcca actacgagcg aagcggagcg
     8881 gcgctaaagc gccaactacg agcgaagcgg agcggcgcta aagcgcca
//
LOCUS       DAOMQG010000113         8807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3413_length_8807_cov_11.369059, whole genome shotgun sequence.
ACCESSION   DAOMQG010000113 DAOMQG010000000
VERSION     DAOMQG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3413_length_8807_cov_11.369059"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..2015)
                     /locus_tag="PLC06_07955"
     CDS             complement(171..2015)
                     /locus_tag="PLC06_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HRO90415.1"
                     /translation="MSDQSGARTDSLVLYKGQPAIIKQVTDKKITIESADGERVNVRP
                     KDVSLLHPGPSPHPSRLKVPDGDPQTAWELLDGAETSLDELAELSFGEYTPHSAWAAW
                     QLVANGVYFEGTPEKIHVHTPAKVAEIMASRESKVAEEREWDAFVARAANGSISPDDE
                     KYIGDVIALALGRTESSRTLRRLDRSQTPESAHELLLSTGRWRPSDNPYPSRQGVTTE
                     QPDFPIEPLPDEPRHDLTHLLSLAIDDIGSNDPDDAISLDGEILWVHVADVAALVSPD
                     SPPDVEARFRAANLYLPEGIVRMLPDALTDMLALGLQETAPALSFALKPDSDGDFELL
                     EIMPSRIRVTRMTYEAAEEQLDTSPLRELMKITERNRARRLAGGAVEIDLPEVKIKAA
                     VNGEVTIRPLPSLQSRELVREAMLMAGEAVGRFGVANEIPLSYTVQEPPLEDESLPKD
                     LTGAPPSTMWAQRRLMQRSRPSTAPGRHSGLGLDVYVQVTSPLRRYLDLLAHQQLRAH
                     LKGGQLLSTAEVTQRIGATDSIAGAVRTTERLSNQHWTLVYLLQHADWQGEAIVVENK
                     PGRDVLLIPELAWETEIYRRPSRPLDSVVALSLDSVDLANRTAHFRII"
     gene            complement(2025..2843)
                     /locus_tag="PLC06_07960"
     CDS             complement(2025..2843)
                     /locus_tag="PLC06_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO90416.1"
                     /translation="MTSFDGKNVLITGSASGIGRLMAQKIAAKGGNVILWDLNLPGLQ
                     SLHDQLARWGYNSAAYYQCNLSNRDEIKETAQKVLAEQGPVDILINNAGIVSGKPLLE
                     LSDEAIERTFDVNTLSLFWTTRAFLPAMLEKNSGHIVTIASAGGLIGVPRMTDYSASK
                     FAAVGFDDSLRVELKRLGHGNIRTTIVCPYYINTGMFDGVKTRVPWLLPIMEPEDAVN
                     KIINAIEKDKRRLIMPPFALITYPGRLLPVPMFDKLAEFFGINSSMDDFTGRNK"
     gene            complement(3005..4849)
                     /gene="glmS"
                     /locus_tag="PLC06_07965"
     CDS             complement(3005..4849)
                     /gene="glmS"
                     /locus_tag="PLC06_07965"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRO90417.1"
                     /translation="MCGIVGYIGSRQAPHVVLHGLKRLEYRGYDSAGIAALDDAGQIA
                     IRRDVGKLSNLETTLLNSPLSGQISIGHTRWATHGAPTQRNAHPHLSMNGRVVLVHNG
                     IVENFKELRQELSEEGVTFQSDTDTEVIVQLAERYMAAGLSLEEAARHTLQHLRGANA
                     VVMMSIDSPDRLVCARIGNAGGITLGVGDDEMFIASDIPAILEYTRRMIFLDSHQMAI
                     ITRDGFEVMSLDGRPIAPEIQTIAWDPVSAVKGEFKHFMKKEIFDQPRALIDTLGGRI
                     DFEAGIVNLPEMNLTPELVNKIDKLFIIACGTSYYAGLVGKYLIETLARLPVEVEYAS
                     EFRYYNPIVDEHTAVLSITQSGETADTLAAAQQARDNGATLWAIVNAIGSQSMRLADG
                     SIAMQAGPEICVASTKAFTTSIVDLYLLACALGNLRGTLSADRLQQLVSDVVHLPSLI
                     GEVLERDDEYQALADHFHKAQDFLFLGRGINYPIALEGALKLKEISYIHAEGYPAGEM
                     KHGPIALIDEAMPVLAIVTKDALYDKMISQVQQAKARGGVVIALATEGDELIHQEADY
                     VIYVPEAPPLLAPVVNVVPLQLLSYYIAVRRGADVDQPRNLAKSVTVE"
     gene            5552..6880
                     /gene="rseP"
                     /locus_tag="PLC06_07970"
     CDS             5552..6880
                     /gene="rseP"
                     /locus_tag="PLC06_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRO90418.1"
                     /translation="MFNSNLLWAIGGFLVILTPIVILHEFGHFWAAKLSKIRVEEFGF
                     GLPPRAVKLFERGGTIYSLNWIPVGGFVRPAGEDDPNVPGGLASASKRARLFVLAAGS
                     GMNFVTAILFFWLALVLGAPTVQVGAISPGSPAEVGGLQTGDVILKAGGTVVVNSSTL
                     SNAISGQAGTATDFEIIRNGERQTLSVTPRRPGEYDPATEDSLGVGIPIQISGISPGS
                     PAESGGLQVGDIIIEVDGNIVMNSGMLIDIISASGGKAIDLVVMRGGEEQQITVTPRR
                     EGEFDPNTEGSVGIGLSPVQGPSIRRGPIEAVSGSFNFMWEYISLYARLPSMLINGEI
                     TAREARPVSIVGISQIAGEAAESSAEGRTLYPVLTMASFISIALGLTNLLPIPALDGG
                     RILFVLIEAIRGRRVDPEREGMVHVIGMLVLLALMAVLIIQDLINPILPF"
     gene            6890..7888
                     /locus_tag="PLC06_07975"
     CDS             6890..7888
                     /locus_tag="PLC06_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRO90419.1"
                     /translation="MTSQQEMPFASVLEALFSAEDLPISLLYRLSDLSDADFERFKRG
                     WSAVDEDRRVAIARHMSEIVEESYLLDFSPVFAYMFSDESAAVRVAALDGLWDSEEQS
                     LIPMILDLLATDKDHSVRAAAARALAHYILLAEWGQISNSYTDKIVDGLLAAYDDPRS
                     PLDVKRATLEAVSASAHPRVPVMITDAYEEGSNDLQLSAIFAMGVSADERWLPMLEQE
                     LHSPSPDFRAEAARACGIIGGHSSIEALEQLINDEDQEVGMAAILALGQIGGDRVFAL
                     LSHLAEDPNYEDYYEVIEDALDELEWMDSSFDMLAFPDEENHDDDEDDILDQLRLN"
     gene            7885..8796
                     /locus_tag="PLC06_07980"
     CDS             7885..8796
                     /locus_tag="PLC06_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRO90420.1"
                     /translation="MNVFLYVLTQNILPIMLVASFGYLLRKRLNLHIGTLSSVVFNIL
                     SPCLVFASLANSRLPGDELIELVGFSALNILVMGGLALVLARLLKLERVELATFLIVV
                     MFVNGGNYGLTLLQLRYGDDGLSRGVVYYVTSTVLVYTLGVVIASMGRVNWRDTLKRM
                     ARLPAVYAAVLAIVVYSFRIPIPAPLMSGITIAGNGAIPVMLLVLGMQFAEMHTNDGG
                     RYVWPAVGLRLIGGPLVGLLIATVLNLQGLGRSAMIIESAMPTAVISLILASEFGLPT
                     SNVARIVVLSTLASPLTIAATITLLGL"
BASE COUNT         1706 a         2431 c         2652 g         2018 t
ORIGIN      
        1 aagtctggtt tggcgtgagt aacgcctcga tcagccgctt cgagatcgag aatggtcaaa
       61 ttgccgttcg ctatctcaat cgggtggatt atctgccgga tgaactgatt tccggctgaa
      121 agccggcgcg acgcaccgat cctttgacac ctatcggagc cgatgagcga tcagataatt
      181 ctgaagtgcg cggttcgatt ggccagatcg acgctatcga gagaaagggc caccacagaa
      241 tccagcggcc gtgacggtcg acgatagatt tctgtctccc acgccaattc ggggatcagc
      301 agcacatctc gccccggctt gttctcgacc acgatagctt ccccctgcca gtccgcatgc
      361 tgcaacagat agacgagcgt ccaatgctga ttggagagcc gttcagtcgt gcgcacggcg
      421 ccggcgattg aatcagtcgc gccgatccgt tgggtgacct cggccgtgga gagaagctgc
      481 ccccctttga gatgggcacg caattgctga tgagccaata gatcgagata gcggcgcaat
      541 gggctggtca cttgcacgta gacgtcaagt cccaaaccgg agtggcgccc cggcgccgtc
      601 gatggccggc tgcgctgcat gaggcgacgc tgtgcccaca tggtggaggg aggcgctccg
      661 gtcaggtctt ttggcaggga ttcatcttcc agcggtggct cctggaccgt gtaactcagc
      721 ggtatctcgt tagccacgcc aaaccgccct accgcttctc ctgccatcag catggcctcg
      781 cgcaccaact cgcggctttg cagggacggg agcggcctga tggtaacctc gccattgacg
      841 gccgccttga ttttcacctc ggggagatcg atctcgaccg cgccgccggc aagacgacgc
      901 gctcgattcc gttcggtgat cttcatgagt tcgcgcaaag ggctggtatc caattgctct
      961 tcggccgctt cgtaggtcat tcgcgtgacg cggatacgac tcggcatgat ttccaggagt
     1021 tcgaagtcac catcgctatc aggcttcaac gcaaaggata atgccggggc tgtctcctgc
     1081 aaacccaagg ccagcatgtc tgtgagcgca tccggcagca ttcggacgat gccctccggc
     1141 aggtagagat tggcggcccg gaagcgcgct tccacgtcgg gcgggctatc gggggagacc
     1201 agcgcggcga cgtcagccac gtggacccac agtatctcac cgtcgagact gatggcatca
     1261 tcggggtcgt tgctgccgat gtcatcgatg gccaacgaca gcaaatgggt taggtcgtgc
     1321 cggggttcat caggcaacgg ctcaattggg aagtccggct gctcggttgt cacgccctga
     1381 cgggaggggt aggggttgtc ggacggccgc cagcgaccgg tggataggag caactcatga
     1441 gccgattccg gcgtttgcga acggtcgagg cgccgcaggg tgcggctgct ctccgttcgt
     1501 cccagagcca gggcaatcac gtcgccgata tatttttcgt catcagggga gatgctgccg
     1561 ttcgccgcgc gggcgacgaa ggcgtcccac tcgcgctctt cggccacctt tgattcgcgc
     1621 gatgccatga tttcagccac tttggccgga gtatggacgt ggattttctc cggtgtgccc
     1681 tcaaaataaa cgccgttggc gacgagctgc caggctgccc aggccgaatg aggtgtatat
     1741 tcgccgaagg aaagctcggc taactcgtcc agcgaagtct ctgccccatc cagcagttcc
     1801 caggcagttt gggggtcgcc gtcgggaacc ttgagccggc tcggatgagg gctggggccg
     1861 ggatggagca gagagacgtc cttcggtcgg acatttacgc gctctccatc ggccgattca
     1921 atcgtgattt ttttgtcggt gacttgtttg atgatggccg gttggccttt gtagagaacc
     1981 aggctgtctg tcctggcacc tgattgatcg ctcactgagc tcacttattt gttgcggccg
     2041 gtgaaatcgt ccatcgaact gttgataccg aagaactcgg ccagcttgtc gaacatgggg
     2101 acaggcagca ggcggccggg gtaggtgatg agagcgaacg gcggcatgat tagacggcgc
     2161 ttgtccttct caatggcgtt gatgatcttg ttgaccgcgt cctccggctc catgatcggc
     2221 aacagccagg gaacgcgggt cttaaccccg tcgaacatac cggtgttgat gtagtagggg
     2281 cagacgatcg tcgtgcggat gttgccgtgg cccaggcgct tcagttcgac gcgcaaggag
     2341 tcatcgaagc cgacggcggc gaatttgctg gcgctgtagt cggtcatcct gggcacgccg
     2401 atcaggccgc ctgccgaagc gatggtcacg atgtggccgc tgttcttttc cagcatggcc
     2461 ggcagaaatg cgcgcgtcgt ccagaagagc gacagcgtgt tcacgtcgaa cgtccgctcg
     2521 atagcctcat ccgatagctc cagaagcggt tttccggaaa cgatccccgc attattgatg
     2581 aggatatcga ccgggccttg ctcagccaga accttttgtg cggtctcctt gatctcatcc
     2641 cgattgctca agttgcattg ataataagcc gcggagttgt atccccagcg ggcgagttgg
     2701 tcgtggagtg actgaaggcc gggcaaattc agatcccaca ggatgacgtt gccccctttc
     2761 gcggcgattt tctgcgccat gaggcggccg atgccgctcg cgctaccggt gatcaataca
     2821 tttttgccat caaaagaggt catgccagct ccacccgaga aaattggatt gattaagaga
     2881 ttatagacgg acggggaaga tcatcaaggg acaaacatgc gccggataat tattgaagca
     2941 ccggatggct gggcgaatcg gttgatacca atgcaactcg cccagccgcc gatgcctgct
     3001 ttttctattc taccgttacg ctcttggcca gatttcgtgg ctgatcgacg tctgccccac
     3061 ggcgaacggc gatgtagtag ctgagtagct gcagcggaac tacattgacg accggggcca
     3121 gcagcggtgg cgcttccgga acgtagatca catagtccgc ttcctgatgt atcagctcgt
     3181 ccccttcggt cgccagcgcg atgactacgc cgccacgggc tttggcttgc tggacctggc
     3241 tgatcatctt gtcatacagc gcgtctttcg tgacgatggc caggacgggc atggcctcat
     3301 cgatcagggc aataggccca tgcttcattt cgcccgccgg gtaaccctcg gcatggatgt
     3361 agctgatttc cttcagcttc agagctcctt ccagagcgat ggggtagttg atgcctcggc
     3421 cgagaaagag aaagtcctgg gctttgtgga agtgatcggc caatgcctgg tattcgtcgt
     3481 ccctctcaag cacctcgccg atcagggagg gcaggtggac gacgtcgctg accagttgct
     3541 gcaaccggtc ggcggagaga gtacccctca ggttgcccaa ggcgcaagcc agaagataga
     3601 gatcgacaat ggaggtggtg aacgccttcg tgctggccac gcatatttcc gggccggctt
     3661 gcatggcgat ggaaccgtcc gccagacgca tggattggct gccgatggcg ttgacgatcg
     3721 cccagagcgt ggccccgttg tcgcgggctt gctgcgccgc ggccagcgtg tcggccgttt
     3781 cgccgctctg ggtaatcgag agtacggcgg tatgctcatc cactatcgga ttgtaatagc
     3841 ggaactcgga tgcgtattcc acttcgaccg ggaggcgagc caaggtttcg atgaggtatt
     3901 taccaaccag accggcataa tagctggtgc cacaggcgat aatgaataat ttgtcgatct
     3961 tgttgaccag ttcgggcgtc aggttcatct ccggcaggtt gacgatgcct gcctcaaaat
     4021 caattcggcc gccaagcgtg tcgatgagcg cgcggggctg gtcgaatatc tcttttttca
     4081 tgaagtgttt gaattcaccc ttgacggcgc taaccgggtc ccaggcgatg gtctgaatct
     4141 ccggggcgat ggggcggccg tcgagagaca tcacttcgaa cccgtcgcgc gtgatgatgg
     4201 ccatctggtg gctgtccaga aaaatcatcc gtcgggtgta ttccagaata gccgggatat
     4261 cggacgcgat gaacatttca tcgtcgccga cccccaatgt gatgccgccg gcgttaccga
     4321 tgcgggcgca caccagccga tcgggggaat cgatgctcat catgacgacg gcgtttgcgc
     4381 cccgtaaatg ctggagtgtg tgccgggcgg cctcttcgag ggataaccct gcggccatgt
     4441 agcgttctgc gagctggacg atgacttcag tgtcggtatc cgactggaag gtcacacctt
     4501 cctccgatag ctcctgccgg agttccttga aattctcgac gatgccgtta tgaaccagga
     4561 cgactcgccc gttcatgctc aaatgaggat gggcgttgcg ctgggtcggc gcaccgtgag
     4621 tcgcccaacg cgtatggccg atgctgattt gacctgacag gggagagttg aggagtgtcg
     4681 tctccagatt ggacagcttg ccgacatcgc gacgaatggc gatttgccct gcatcatcca
     4741 gtgccgcaat tccggctgag tcgtagccgc ggtattccag gcgcttcagg ccatggagca
     4801 cgacatgagg agcctggcgt gaaccaatat aaccaacaat accacacatg gttaacctcc
     4861 atacgtggca caatcatggc cgcggctact ccgggtgaaa gacttccctc cagggggaat
     4921 cattcggccg tcggccacag aatgtgccct gcaggcgttc ccattcgatt gttggcaatg
     4981 ggggacgccc cttcggttga tgtaattatc cggtcacgcg ccgggaagtc tgtcggccgg
     5041 ttacccgccc ggtttgctgc tcggcttggt tgaaggggca aaattcagaa tgtgaccaac
     5101 tctgggaggc atccgctgat ctctcgattt tcaccgggag acgttagctt tctcgatctg
     5161 gtagaaattt catcgatgaa agcatgcgtc tcgccggttt aacctcgcgt cgccgcgagg
     5221 aacctccacc tcgtcacccg ctcgaccttt gtcggtaacg ttcaagtcgt gcgggccatg
     5281 cgctattcag ccccgcggac ggccgcaccg gagtgattgg ccgccctgta ttcaattgtt
     5341 gaattacagg ttatcgatcc gaaaggggat tgtcaagcaa gcgttcgtga aattcacctt
     5401 ttcatgagcg ttagagtggt gttagatcgc gtgatggctt cttgcgatcg cttgtccagt
     5461 agacatataa tctcctgtaa gctcgcactc ggttgcttac actcacgtaa tctcatcggg
     5521 gcggcaagat acaagcagct gagtaaccaa tatgtttaat tcgaatctac tttgggccat
     5581 tggcgggttc ctcgtcatcc tgacccccat cgtaattctc catgagttcg gccacttctg
     5641 ggccgcaaag ctgagcaaga ttcgcgtcga agaattcggc ttcggcctgc ctcctcgcgc
     5701 ggtcaagcta tttgagcgtg gcgggacgat ctactcgtta aactggatcc ccgtgggcgg
     5761 ctttgtgcgg ccggcgggcg aggatgatcc caacgtgccg ggtgggctgg cttccgcctc
     5821 caagcgagcg cggctatttg ttctggctgc cggctcaggc atgaacttcg tcacagccat
     5881 cctgtttttc tggctggcgc tcgtgttggg tgcgccgacg gttcaggttg gcgcgatcag
     5941 tcccggatcg ccggctgaag tcggtggcct ccaaaccggc gatgtaattc tgaaggccgg
     6001 aggcacagtt gtcgtcaata gcagcaccct gagtaatgcg atttcagggc aggcgggtac
     6061 ggcgactgat tttgaaatca ttcgcaatgg cgagaggcaa acgctttcgg tcacgccacg
     6121 gcggccggga gagtatgacc ctgccactga agattcactc ggtgtcggaa tccccattca
     6181 gatcagtgga atcagccccg gttcgcccgc ggaaagcggc gggcttcagg tcggcgatat
     6241 catcattgaa gtcgacggca atatcgtcat gaacagcggc atgctgatcg atatcatttc
     6301 ggcgagtggg ggcaaggcga tcgatctggt agtcatgcgc ggcggcgagg aacaacagat
     6361 caccgttacc cctcggcgag agggtgagtt tgatccaaac acggaaggct cagtaggaat
     6421 tgggctaagc cccgtgcaag gaccgtccat tcgtcgcgga cccatcgagg cagtctcagg
     6481 ctccttcaat ttcatgtggg aatatatatc cctctacgcc aggctcccat ccatgttgat
     6541 caacggagag atcaccgcgc gagaggcgcg cccggtcagt atcgtcggca ttagccaaat
     6601 cgccggggaa gcggccgaat cctcagctga aggccggact ctataccctg tcctgaccat
     6661 ggcctcgttc atcagcatag cgctgggtct gaccaacttg ttgcccattc ccgccctcga
     6721 cggtggccga attctcttcg tgctcatcga agccattcgc ggccgccggg tcgatcccga
     6781 acgtgaggga atggtccacg tgatcggcat gctggttcta ctggcgctca tggctgtttt
     6841 aatcattcaa gaccttatta acccaatact cccgttctaa taaaacaaaa tgacatctca
     6901 acaggaaatg ccttttgcct cggtgctcga ggccttgttt tcggcagaag atctgccaat
     6961 ctctttacta taccgtctgt ccgatctgtc ggatgcggat ttcgagcggt tcaagcgagg
     7021 ttggagcgcc gtcgatgagg atcggcgtgt ggccatcgcg cggcatatgt ccgaaatcgt
     7081 cgaggaaagc tatttgctcg actttagccc ggtctttgcc tatatgttta gcgatgaatc
     7141 ggccgcggtt cgcgttgctg ctttggatgg cctgtgggac tccgaggagc aatcgctcat
     7201 cccgatgatt ctcgatttgc tggcgactga caaggaccac agcgtccgcg cggctgcggc
     7261 tcgggcattg gcccattaca tcctgctggc cgaatggggg caaatcagta attcctatac
     7321 cgacaagatc gtggatggcc tgttggcggc ttatgatgac cctcgcagcc cgttggatgt
     7381 gaagcgggca acgctggagg cggtgtctgc ctcggctcat ccgcgtgtgc cggtgatgat
     7441 caccgacgct tatgaagaag gctctaatga ccttcaattg agcgctatct tcgccatggg
     7501 tgtttcggcc gatgagcgct ggttgcccat gcttgaacag gaattgcaca gcccatcacc
     7561 cgattttcgc gccgaggcgg ctcgcgcttg cggcatcatt ggcggccatt ccagcatcga
     7621 ggcgttggag cagctcatta acgatgagga tcaggaggtt ggtatggctg ccatcctggc
     7681 tttgggtcag attggggggg acagggtatt cgcgctgttg tcccatctgg cggaagatcc
     7741 caattatgaa gattactatg aggtcatcga ggatgcgctc gatgaactgg agtggatgga
     7801 cagctctttc gacatgctgg cattccccga tgaagagaat catgacgacg atgaggatga
     7861 catccttgac cagctgcgtc tcaattgaac gtctttctct atgtcctcac ccaaaatatc
     7921 ctgccgatca tgttggtagc ctctttcggc tacctgctgc gtaaacgact caatctccat
     7981 atcggcacgc tatccagcgt tgtctttaat atcctcagtc cctgcctcgt gtttgcctcc
     8041 ctggcgaatt cccggctgcc gggggacgaa ctgatcgagc tggtcgggtt ttcggcgctg
     8101 aatatcctgg tcatgggtgg gctggctctg gttttggctc gtttgctcaa actggaacgg
     8161 gttgaactgg cgacgtttct gatcgtcgtc atgtttgtaa acggggggaa ttatggactc
     8221 acgctcttgc agcttcgcta cggagatgac gggttgtcgc ggggtgtggt ctattatgtg
     8281 accagcacgg ttctcgttta cacgctcggc gtcgtgatcg cgtcgatggg gcgggtgaac
     8341 tggcgtgaca cgcttaagcg gatggcacgt ttgccggccg tctatgcggc cgttctggcg
     8401 atcgtggtct acagttttag aatacccata ccggctcccc tgatgagtgg catcaccatc
     8461 gccggaaatg gggcgatacc tgtgatgttg ctcgtgttgg ggatgcaatt tgccgaaatg
     8521 cacacgaacg atggcggtcg atatgtatgg cccgcggtag gcttgcggct catcggcggg
     8581 ccgttggtgg gtctgctcat tgcgaccgtt ttgaacttgc aaggccttgg acgcagcgcc
     8641 atgatcatcg agtcggccat gccaacggcc gtgattagcc tgattttggc ttccgagttt
     8701 ggtttgccca catccaatgt ggcgcgtata gttgtgctat cgacactagc cagtccactc
     8761 acgattgctg caacgatcac gttgttggga ttatgatcac gccggcg
//
LOCUS       DAOMQG010000114         8787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3425_length_8787_cov_5.563559, whole genome shotgun sequence.
ACCESSION   DAOMQG010000114 DAOMQG010000000
VERSION     DAOMQG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3425_length_8787_cov_5.563559"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2874)
                     /locus_tag="PLC06_07985"
     CDS             complement(<1..2874)
                     /locus_tag="PLC06_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO90421.1"
                     /translation="MSHHFIGKRLALVALCLGLLIALGRVPTAFAAACTTVCYVDAAT
                     GSDANDGDTAATAKKTIQAAVNQVTAGGQVYVLDGLYPETVTVNKAVTITGNGPANTT
                     VDPASGNGFGITASNVTIEDLKITDAAQGARLSGTISGITFDNVHFVNNTSRGIEMQT
                     PTTDLITNVQVLNSLFDNNSLGMNMSTSTRVDGILIENTTFQNHANSGFQQNNGNNVG
                     WVKNLTVRDSTFNNNGTTSGSAGVYAEEFSNVLIEDSTFTGNRYGVNLFDYYNLAAST
                     TTNVVIRNNTFTDHKASTIALRSITSDPSAQLFLIDNNTINQNVGVLAGATQAHIAVS
                     LSSANPNGAVDVIDNDLTFSGTFLLANSATYGIYLAGRAEDVEIEGNTIDGGGVGTNG
                     AAIPSSGIRIVTSVFQTNANINITRNLINNFVNGVSIHDGNNAMGGIQANSVVKINRN
                     DLSGNTAYGIQSGPATATNGKCNWWGDASGPGPVGPGSGSNVSTDVTYAVWLFNNNLN
                     GGNVTNIDTGEVFCSIQEAIDDSDTLNGHTIVASAGVYQENVTVHKSVTLNGANAGVS
                     PNDPITPLNPNGARGPESELAVTGNGVAFKIHAPDVTIDGFKFTDSAVDQTHIQMPLI
                     ATGWNYGGEGPGFKVVNNLFSETSRVLFYSNGPNQITDGTIDNNRVQDPGRADTNCGT
                     APATAPSSCGYQLFNLWRIDGLSFQGNVTAANPGSGDRIRTLNVFNSTEVIVADNTIR
                     NTCIYNCFNIAAGTSGNVEVTGNDVVTDVGSVVQIHSSWTTAGATINVHHNTFTTGAD
                     FPIVVDAVNADLDNVHINRNVLIGDFHARNGTPPSLPGTETLDITCNWWGGVGGPNKP
                     NGLFGPLDYTPWLYTNDLDGPCYIGGTIEIEKVAEGGGNTVFEFDLSWTTPITLTNGG
                     SYVTSPPLQAGSYSISEINLPTGWSQQSATCDNSATTPVETVNP"
     gene            3340..4944
                     /locus_tag="PLC06_07990"
     CDS             3340..4944
                     /locus_tag="PLC06_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90422.1"
                     /translation="MDRRALFALLVGALLMGGALRGAAAGERSPRPIVAAPNGPAQLA
                     LPMQTPGPAGGSVWLPMDYLNGGNAIKTIFFVLDYDATLLTLDPTDADSDGIPDDLTI
                     HLPDDYVALVENELIGPSGRLQVLIYSTSPAAVRIPSGRLIDLLLDVAMVDAPQATDI
                     IFIGSPAPFFATADNKLVDGSFIGGSVAIDFLPTYTPTATMTPTPSPTLTPTDTPTIT
                     PTPTDTPTPTPTVTGTPPTATPTPTNTPAPTSTPTATPTVTGTPPTATPTNTPTSTPT
                     ATPTASPTPSATSTPTPAPLYLPAVVFGESPTPTATPTITPSATATATSPATATLPAS
                     PTSAATATPRPTNTPAATGTPAPPRCVNVLANGGFEDNTAWQINNTVYPAGFVTFPVF
                     AGARALRAGIVHPPDNIRSYSSAQQTLFIPAGPPSVKLSFQLFATTTGTRAALTPPPI
                     VPTSLLDRAALADDVQMVLLFDSSGRQHVLLFQREWYNEWRRHEIDLTPFRGQVVTLY
                     FGVFNNGTGGVTGMYVDEAAVSYCLP"
     gene            5046..7190
                     /locus_tag="PLC06_07995"
     CDS             5046..7190
                     /locus_tag="PLC06_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRO90423.1"
                     /translation="MYSQRIANELNVRPQQVAATIALLDDGNTLPFIARYRKEATGSL
                     DEEQIRHIAAEIERLRALDDRRAAVIASVAEQGKLTPELQAQFDAAETLHALEDLYAP
                     YRPKRRTRASIAREKGLGPLADLILAQPVTNRTAEELARPYLSDDLPTPADVLAGARD
                     IVAEAISDNAAVRGRLRERAMQVGLLSSTLIDGAADEKGVYTLYYDFAAEIPRVRPHQ
                     VLAINRGEAGKVLRVAVVVPEAEWLLAIRGRFRPDRRSPLAGEMQQAIDDAAKRLLLP
                     AVERDVRRALSGTAERHAIEVFARNLRALLTQPPMAGRVAGHAVAGIDPGFRTGCKVA
                     IIDPTGKVLDTATIYPHPPQKKADDARRALIDLIERHNVALVAIGNGTAGRETEQLVA
                     GLTRERAGLHYIIVNEAGASVYSAGTLARAELPDMDVSMRGAVSIARRLLDPLAELVK
                     IDPRSIGVGLYQHDVDQKQLAASLDGVVESVVNRVGVEVNTASPALLEHVAGIGPKLA
                     GAIVAHRDANGPFPGRAALRAVAGLGPKAYEQAAGFLRIRGGNNPLDAGAIHPESYKV
                     AEKVMARAGMAADTPAREREALLAKLCEASPLEQLAAELDTGAPTLADILDQLVRPGR
                     DPRDDLPPPLLRSDVLSIDDLRPGMLLNGTVRNVVDFGAFVDIGVKHDGLLHKSRLPR
                     AVSLQPGDVIAVTVAEIDKERGRIGLEYGVDN"
     gene            complement(7470..>8787)
                     /locus_tag="PLC06_08000"
     CDS             complement(7470..>8787)
                     /locus_tag="PLC06_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5979 domain-containing protein"
                     /protein_id="HRO90424.1"
                     /translation="KFGPVSVTLDDGQSHTFPGLPAGDHTIAELVPGGWTLDDIDCGS
                     ATATRDGDAISITLAAGDDVTCVFTNVQYGTITVIKQAGDVGDDFEFDPSWSDDNFLL
                     GSGGAHTSDPLPAGTYSVVEVNLPPGWSQQGATCDNATTTPVETVSPTSIPVADGDEW
                     VCTFTNVYTPPPTNVCNVQDSSALWTDIIGLGMGSPKKHKAQVKLNIPNYTAVDSLYG
                     QMVAKNVGHANYVRFYMPGKNNYVQVNTITSPAGMSGGSFWYGADLTSQGPLNWVKGR
                     WFLQGSGTKGHIPRALVLYPTYESATNTYVNIWNTYDAAEGEVHWDIANGWVPTRVIT
                     EAIAPPNGPTTFNVELAVVDNDKDARPVWVTVAAGGVTQTQMPTNPSNGDQLNLMTFT
                     LANVPPGTDEITITVYSPSVALDGIEGDSAALVGMTANYMCAPLPQ"
BASE COUNT         1436 a         2924 c         2815 g         1612 t
ORIGIN      
        1 cgggttgacc gtctccaccg gcgtcgtggc gctgttgtcg cacgtcgcgc tctgctgcga
       61 ccagccggtg ggcaggttga tctcgctgat cgagtagctg ccggcctgca gcggcgggct
      121 ggtgacatac gagccgccat tggtcagcgt gatgggcgtg gtccaactca ggtcgaactc
      181 gaacacggtg ttgccgccac cctcggccac cttctcgatt tcgatcgtcc cgccgatgta
      241 gcaggggccg tcgaggtcgt tggtgtagag ccagggggtg tagtcgagcg ggccgaagag
      301 gccgttgggc ttgttcgggc cgccgacgcc gccccaccag ttgcaggtga tatcgagcgt
      361 ttccgtgccg ggtaggctgg gcggcgtgcc gttgcgggcg tggaaatcgc cgatgaggac
      421 gttgcggttg atgtgaacgt tgtccagatc ggcgttcacg gcatcgacga cgatggggaa
      481 gtcggcaccg gtggtgaatg tgttgtgatg aacgttgatc gtggccccgg cggtcgtcca
      541 ggagctatgg atctggacta cgctgccgac gtcggtcacc acgtcattgc ccgtcacttc
      601 cacgttgccg ctggtgcccg cggcgatgtt gaagcagttg tagatgcagg tattgcggat
      661 ggtgttatcg gcgacgatga cctcggtcga gttgaagaca ttcagcgtgc ggatgcggtc
      721 gccactgccg gggttggcag ccgtcacgtt gccctggaag gacaaaccgt cgatgcgcca
      781 cagattgaac aactggtagc cgcagctgct gggcgcggtc gcgggcgcgg tgccgcaatt
      841 ggtatcggcc cggccggggt cctgaacacg gttgttgtca atcgtgccgt ccgttatttg
      901 gttggggccg ttggaataaa acaggacgcg gctggtctcg gagaacaggt tgttgaccac
      961 cttgaagccg gggccttcgc cgccgtagtt ccagcctgtg gcaatgagcg gcatctggat
     1021 gtgggtctga tccacagccg aatcggtgaa cttgaagccg tcgatggtca catcgggcgc
     1081 atgaattttg aaggccacgc cgttgccggt gacggccagt tccgactcgg ggccgcgcgc
     1141 gccgttgggg ttcaacgggg tgatcgggtc gttgggcgaa acgccggcgt tggcgccgtt
     1201 cagcgtcacc gacttgtgga cggtgacatt ttcctgatag acgcccgccg acgccacgat
     1261 agtgtggcca ttcagcgtgt cgctgtcgtc gatggcttcc tggatggagc aaaatacttc
     1321 gccggtgtcg atgttggtga cattgccgcc gttgaggttg ttgttgaaga gccacacggc
     1381 ataagtaacg tcggtgctga cattggagcc gctgccgggg ccgaccgggc cggggccgct
     1441 cgcgtcgccc caccagttgc acttgccgtt ggtcgccgtc gccgggccgc tctggatgcc
     1501 gtaggcggtg ttgccgctca ggtcgttgcg gttgatcttc acgaccgagt tggcctggat
     1561 gccacccatc gcgttgttgc cgtcgtggat cgaaacacca ttcacgaagt tgttgatcag
     1621 gttcctggtg atattgatgt tggcgttggt ctggaagacg ctggtgacga tccggatgcc
     1681 cgagctgggg atcgccgccc cgttggtgcc cacgccgccg ccgtcgatcg tgttaccttc
     1741 aatttccacg tcttcggcgc gtccggccag atagatgcca taggtcgccg agttggccaa
     1801 caggaacgtg ccggagaagg tcagatcgtt atcgatcacg tccaccgcgc cgttggggtt
     1861 ggccgatgag agcgacacgg cgatgtgggc ctgcgtcgcg ccggccagca cgcccacgtt
     1921 ctgattgatc gtgttgttgt cgatcaggaa cagttgggcg ctggggtcgc tggtgattga
     1981 gcgcaaggcg atggtcgacg ccttgtggtc ggtgaaggtg ttgttccgga tgaccacgtt
     2041 ggtcgtcgtc gacgccgcca ggttatagta atcaaacagg ttgacgccgt agcggttgcc
     2101 ggtgaaggtc gagtcctcga tcaggacatt gctgaactcc tcggcataga cgccggccga
     2161 gccgctggtg gtgccgttgt tgttgaaggt gctgtcgcgc accgtcaggt tcttcaccca
     2221 gccgacgttg ttgccattgt tctgctggaa cccggaattg gcgtggttct gaaaggtggt
     2281 gttttcgatc agaatgccgt cgaccctcgt ggaggtcgac atgttcatgc ccaggctgtt
     2341 gttgtcgaag aggctgttta atacctggac gttggtgatg agatccgtag tgggggtctg
     2401 catctcgatg ccccgcgacg tgttgttcac aaagtgaacg ttgtcgaagg tgataccgga
     2461 gatggtcccc gacaaccgcg cgccctgggc cgcgtcggta atcttcagat cctcaatggt
     2521 gacattggaa gcggtgatac cgaagccgtt gcccgacgcc ggatcaaccg tcgtgttggc
     2581 cgggccgttg ccggtaatcg tcaccgcctt gttgaccgtg acggtttccg gatagagacc
     2641 gtccagcacg tacacctgcc cgccggcggt cacctgattg acggccgcct ggatcgtctt
     2701 cttggccgtc gccgcggtgt cgccgtcgtt ggcgtcgctg ccggtcgcgg cgtcgacata
     2761 gcagacggtg gtgcaggccg cggcgaacgc cgtgggcact cgcccaagcg cgatcagcag
     2821 gccgagacac agggccacga gcgccaaccg ctttccaata aagtgatgac tcattacatt
     2881 ttcctccaaa ctgacaacca acaacgataa actgataagc cgtggcacta acgggcgacc
     2941 ggctcatgcc gaacgcccat agtccagccc ggcgcgacga ggcgcgccga agcggctttt
     3001 cgattttcac agattgtggc aactatcaac tgcgcggcct actgcgatga gaggggtggc
     3061 tccgggcggt caatgggtaa ccaggtcgac tggttctaat gataactagc tgatcggttt
     3121 tctcctcccc gctccggttt gtgggagcgg gcgatatgat ccctgctgcg gccgctcgct
     3181 cgtcgtttcg tcgtgactgg attgcacgaa ggacaaggcg cggctctcat attatactaa
     3241 tgcaggattc ttacagtttg cattacagtc ggacgcgact ccatttcacg tccccttccc
     3301 tgacaacccc catcccgcgg catataatgg gggatgcgta tggatagacg tgctttgttt
     3361 gccttgttgg tgggggcgct gttgatgggt ggcgcactcc gtggggcggc cgccggggag
     3421 cgcagcccgc ggccgatcgt cgccgcgccg aatggcccgg cacaactggc cctgcccatg
     3481 cagacccctg gcccggccgg gggcagcgtc tggctgccga tggactacct caacggcggc
     3541 aacgccatca agaccatctt cttcgtcctc gactacgacg ccaccttgct gacgctcgac
     3601 ccgaccgacg ccgacagcga cggtatcccc gacgatctga ccatccacct gcccgacgat
     3661 tacgtggcgc tggtggagaa cgaactcatc gggcctagcg gccgtctgca agtgctcatc
     3721 tacagcacca gccctgccgc cgtgcgcatc cccagcggcc gcctgatcga cctgctgctc
     3781 gacgtggcca tggtcgacgc cccccaggcc accgacatca tctttatcgg ctcccccgcg
     3841 ccgttcttcg ccaccgccga caacaagctc gtggacggca gcttcatcgg cgggtcggtc
     3901 gccatcgatt tcctgccgac ctacacgccc acggcaacaa tgacgccaac gccgtcgccc
     3961 acgctgacgc caaccgacac gccgaccatc acgcccacgc ccaccgatac gcccacgccg
     4021 acgcctaccg tgaccggcac gccgccgacc gcgacaccca cgccgaccaa cacgcctgcg
     4081 ccgacatcga cgccaacggc aacgcccacc gtgaccggca cgccgccgac cgccacgccc
     4141 accaatacgc ccacgtccac gccgaccgcg acgcccacgg cgtcgcctac gccatcggcg
     4201 acgtctaccc ccacgcccgc gcccctttac ctgccggcgg tggtctttgg cgaatcgccc
     4261 acgccgaccg cgacgcccac gataacgccc tcggcaaccg ccaccgcgac cagtccggcg
     4321 accgcgacgc tcccggcgtc gcccaccagc gcggccacgg cgacgccccg gcctacgaac
     4381 acccccgccg ccaccgggac gcccgccccg ccccgctgcg tcaacgtcct ggccaacggc
     4441 ggcttcgagg acaacacggc ctggcagatc aacaacaccg tctacccggc cggcttcgtc
     4501 accttcccgg tctttgccgg cgcgcgcgcc ctgcgcgcgg gcatcgtcca cccgcccgac
     4561 aacatccgca gctactcgtc ggcccagcag acgctgttca tccccgccgg gccgccctcg
     4621 gtcaagctca gcttccagct gttcgccacg acgaccggca cccgcgccgc cctgacgccg
     4681 ccgcccatcg tgcctacctc gctgctcgac cgggccgcac tggccgacga cgtgcagatg
     4741 gtgctgttgt tcgacagcag cggccgccag catgtgctcc tcttccagcg ggaatggtat
     4801 aatgaatggc ggcggcacga aatcgacctg acccccttcc gcggccaggt ggtgacgctc
     4861 tatttcggcg tctttaacaa cggcaccggc ggcgtcaccg gcatgtacgt cgatgaggcg
     4921 gccgtcagtt attgtttgcc gtagtgacga gtgacgaatg acgagcgcat ctcgtacttc
     4981 tgaacttcat cttcactcgt cactcgtaac tcgcaactct cctgattcct gattcctgat
     5041 tcatcatgta ttcccaacgc atcgccaacg aactcaacgt gcggccgcag caggtggccg
     5101 cgaccatcgc cctgctcgac gacggcaaca ccctgccgtt catcgcccgc tatcgcaagg
     5161 aagccaccgg ctcgctcgat gaagagcaga ttcgccacat cgccgccgag atcgagcggc
     5221 tgcgcgccct cgacgaccgc cgcgccgccg tcatcgcctc ggtcgccgaa cagggcaagc
     5281 tgacgccgga gctgcaggcc caattcgacg cggccgagac gctccacgcc ctcgaagacc
     5341 tctacgcccc atatcgcccc aagcgccgca cccgcgcttc catcgcccgc gagaaagggc
     5401 tggggccgct ggccgacctc atcctggccc agccggtgac caaccgcacg gccgaggagc
     5461 tggcccggcc ctatttatcc gacgatctgc ccacccccgc cgacgtcctg gccggcgcgc
     5521 gcgacatcgt ggccgaggcc atcagcgaca acgccgccgt ccgcggccgt ctgcgcgagc
     5581 gagccatgca ggtcggcctg ctcagcagca cgttgatcga cggcgcggcc gacgagaaag
     5641 gggtctacac cctctactac gacttcgccg ccgagattcc ccgcgttcgg ccgcatcagg
     5701 tgctggccat caaccgcggc gaggccggga aggtgctgcg cgtggccgtc gtcgtgcccg
     5761 aagcggaatg gctgctggcc atccgcggcc gcttccggcc cgaccgccgc tcgccgctgg
     5821 ccggcgagat gcagcaggca atcgacgatg ccgccaagcg gctgctgctg cccgccgtcg
     5881 agcgcgacgt gcgccgcgcc ctgtccggga cggccgaacg ccacgccatc gaggtcttcg
     5941 cccgcaacct gcgcgccctg ctgacccagc cgccgatggc cgggcgggtg gcgggccacg
     6001 ctgtggccgg catcgacccc ggcttccgca ccggctgcaa ggtggcgatc atcgacccca
     6061 ccggcaaggt gctggacacg gccaccatct acccccaccc gccccaaaag aaggccgacg
     6121 acgcccgccg cgccctgatc gacctcatcg agcgccacaa cgtcgccctc gtcgccatcg
     6181 gcaacggcac ggccggccgc gagacggagc agctggtggc cgggctgacc cgcgagcggg
     6241 ccgggctgca ctatatcatc gtcaacgagg ccggggccag cgtctacagc gccggcacgc
     6301 tggcccgcgc cgaactgccc gacatggatg tgtcgatgcg cggcgcggtg tcgatcgccc
     6361 gccgcctgct cgacccgctg gccgaactgg tgaagatcga cccgcgctcg attggcgtcg
     6421 gcctctacca gcacgacgtg gatcagaagc agctggccgc ctcgctcgac ggcgtggtgg
     6481 agtcggtcgt caaccgcgtc ggggtggaag tcaacaccgc ctcgcccgcc ctgctggagc
     6541 atgtggccgg aatcgggccg aagctggccg gggccatcgt ggcccatcgt gacgccaacg
     6601 ggccattccc cggtcgggcg gcgctgcggg ccgtggccgg gctggggccg aaggcgtatg
     6661 agcaggccgc cggattcctg cgcatccgcg gcggcaacaa cccgctcgac gcaggagcca
     6721 ttcacccgga gagttacaag gtggccgaga aggtgatggc gcgggcgggg atggcggcgg
     6781 atacaccggc gagagagcgt gaggcgctgc ttgctaaact atgcgaagca tcgccgctgg
     6841 agcaactggc cgccgaactg gacaccggcg cgccgaccct ggccgacatc ctcgaccagc
     6901 tcgttcggcc cggccgcgac ccgcgcgacg acctgccccc gcccctgctg cgttccgacg
     6961 tgctgtcgat tgacgatctg cggccgggga tgctgctcaa cggcacggtg cgcaatgtgg
     7021 tcgatttcgg cgcgttcgtc gacatcggcg tgaagcacga cgggctgctg cacaaaagcc
     7081 gcctgccgcg cgccgtctcg ctgcagccgg gcgacgtgat cgccgtcacc gtggccgaga
     7141 tcgacaagga gcgcgggcgg attgggctgg agtatggggt tgataattga gagggggagc
     7201 aggggggaaa gggggaaagg gagcacccct gctccctttc ccccctgcac aacggccgtg
     7261 tatgggtagg ggcgggtttc gaacccgccc ctacaaattg aagagcgcgt ccctttcgta
     7321 cttcgtgcct cgtattttag agtgacgaat gacgagcacc ttctgaactc gtcacccgtc
     7381 actcgtcact cgtcactcgt aattcgtcac tcgtcattca taattcgtaa ttcgtaattc
     7441 gtaattcgta attcgtaatt cgtaattcgt cactgcggca gcggcgcgca catatagttg
     7501 gcggtcatgc cgaccagcgc ggccgaatca ccctcgatcc catccagcgc caccgacggc
     7561 gaatagacgg taatcgtgat ctcgtccgtg cccggcggca cattggccag ggtgaaggtc
     7621 atcaggttca gctggtcgcc gttgctcggg ttggtgggca tctgcgtttg cgtcacgccg
     7681 cccgccgcaa ccgtcaccca cacggggcgc gcgtccttgt cgttatccac cacggccagc
     7741 tccacattga aggtcgtcgg cccgttgggc ggggcgatgg cctcggtgat cacccgcgtc
     7801 gggacccacc cgttggcgat atcccaatgc acctcgcctt cggccgcgtc gtaggtgttc
     7861 cagatgttga catacgtgtt cgtcgcgctc tcataggtcg gatagagcac cagcgcccgc
     7921 gggatgtggc ctttcgtgcc gcttccctgc aggaaccagc gtcccttcac ccagttcagc
     7981 ggcccctggg aagtgagatc cgcgccgtac cagaagctgc cgccggacat gccagccggg
     8041 ctggtgatgg tgttcacctg tacatagttg ttcttgccgg gcatgtagaa gcgcacgtag
     8101 ttggcgtggc cgacgttctt ggccaccatc tggccataga gcgaatcgac cgccgtatag
     8161 ttggggatgt tgagcttcac ctgtgccttg tgcttcttgg ggctgcccat ccccagaccg
     8221 atgatgtcgg tccacagcgc ggaggaatcc tgtacgttgc agacgttggt cggcggcggg
     8281 gtatagacgt tggtgaaggt gcagacccac tcgtcgccgt cggccacggg gatgcttgtc
     8341 gggctgaccg tctccaccgg ggtcgtggtc gcgttgtcgc acgtggcgcc ctgctgcgac
     8401 cagccgggcg gcaggttgac ttccacgacg ctgtaggtcc ccgccggcaa cggatcggag
     8461 gtatgcgccc cgccgctgcc gagcaggaag ttgtcgtcgc tccagctggg gtcgaactcg
     8521 aagtcgtcgc ccacgtcacc ggcctgcttg atcaccgtga tcgtgccgta ttgaacattg
     8581 gtgaacacgc aggtcacgtc gtcgccggcg gccagggtga tgctgatcgc gtcgccgtcg
     8641 cgggtggccg tggcgctgcc gcagtcgatg tcgtcgagcg tccagccgcc gggcaccagc
     8701 tcggcgatgg tgtggtcgcc cgcgggcagg ccggggaagg tgtggctctg gccgtcgtcg
     8761 agcgtcacag acaccgggcc gaacttc
//
LOCUS       DAOMQG010000115         8716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3464_length_8716_cov_11.093754, whole genome shotgun sequence.
ACCESSION   DAOMQG010000115 DAOMQG010000000
VERSION     DAOMQG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_8716_cov_11.093754"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..853)
                     /locus_tag="PLC06_08005"
     CDS             complement(122..853)
                     /locus_tag="PLC06_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90425.1"
                     /translation="MNDGVRQMMLAGLRHQYPQDSPEQQTRRLFGLLYGTTITYDIRQ
                     LRENDSMSITPIDTMTTVADTLNDLGIEYVIGGSMASAYYGVSRSTLDVDFVADIKPE
                     HVPALVKTLGRDFYLDEQMIRGAIEHKSSFNLIHLPTMFKVDVFIPGTRPFDHLQLQR
                     RVSSAIGLDGDQRIYLSTPEDIVLAKLDWFDRGGRLSERQWHDILGVLLTQKGQLDIL
                     YLRENAANLGVEDILRKAFQESGTE"
     gene            1129..1587
                     /locus_tag="PLC06_08010"
     CDS             1129..1587
                     /locus_tag="PLC06_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HRO90426.1"
                     /translation="MKLAEALILRADHKKRLELLRERLSRVVKVQEGDLPAEDPADLL
                     GEVDRTVTELTGLIRRINRTNMAVQLDDGRSIADAIADRDDLKLRHSILTGLIQNATI
                     RQDRFTKSEVRFQTTIDVAALQRRADDLAQAYRELDTKIQAANWLFDLSE"
     gene            1910..2188
                     /locus_tag="PLC06_08015"
     CDS             1910..2188
                     /locus_tag="PLC06_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90427.1"
                     /translation="MAIQVKRAMLDVKKYQWTAGEGDDKKSVAHDRIMFSRRQGYEVI
                     PMIQKVVNHFGYETEEDVKRVEEAIRSALPGNVRSRKNVFNWLTTYLA"
     gene            complement(2274..3098)
                     /locus_tag="PLC06_08020"
     CDS             complement(2274..3098)
                     /locus_tag="PLC06_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRO90428.1"
                     /translation="MTAPPLISSTTNARIKQVRALGRRKEREAAGLCVVEGIFHVGEA
                     LASGAAEYLIYAPDILASPFGRELVARAESSAIPTYAVTTEVMESVAGKDNPQGLLAV
                     ARQRLARLEDLSAATHPWMIALIAPQDPGNVGTILRTIDAVGASGLMLVDGGVDPYHP
                     SAIRAGMGAIFRIPVATATWSQLIHWVRREGYHLAGTSAHGRDNYRDTGLYTPPVVLL
                     LGSEREGLTAEQTAACDVLMRLPMHGRASSLNLAVAAGVFLYAMHDSMAANTSHHS"
     gene            complement(3140..3457)
                     /locus_tag="PLC06_08025"
     CDS             complement(3140..3457)
                     /locus_tag="PLC06_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90429.1"
                     /translation="MSMDENGLKLLLEYDVNQDTLQEYYQFVMGQFVPTLQSMGLQMS
                     EAWHTAYGQAPNRLLGFVCGDKQTMDNLLTSDAWDELNDQLEKYVTDLHYKVVPYRGG
                     FQI"
     gene            3641..4804
                     /locus_tag="PLC06_08030"
     CDS             3641..4804
                     /locus_tag="PLC06_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRO90430.1"
                     /translation="MHSCRSSLNCFVPPYMLREIIENGTPEQRQQALRNIVSAEAVRV
                     QREVIAKEGPPAPFLEAAGGLNRVIYTAENGSTLPGRQLRGEGGAPTGDPAADEAYDG
                     AGATHALFYEVFERDSIDGAGMRLDSTVHYRTGYDNAFWNGRQMVYGDGDEDLPESQR
                     IFNRFTIAVDIIGHELTHGVTQHTANLIYQGQSGALNESISDVFGSLVKQRLLGHSAA
                     EADWIIGEGLFTSNVNGIGIRSMKAPGTAYNDPVLGKDPQPGHMRDYVQTQSDNGGVH
                     INSGIPNRAFHVTAMNMGGYAWEKAGLIWYATLKDKLTESATFATAAAKTYEAARDLY
                     GQGSLEQQAVRAGWAEVGIDVGQETPPPPDNGGSRPGCSGSLMSLFRSIIGAR"
     gene            4829..5185
                     /locus_tag="PLC06_08035"
     CDS             4829..5185
                     /locus_tag="PLC06_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90431.1"
                     /translation="MKIYFERSGGFMGRNVSTTVDTNQIPPEQALSLLKKVDYSDFFC
                     LPESLTGGLESASGADQLCYKVTVDVDGIEHTVETSDAGAPAELQPLLDELSSIARGS
                     TIPTGPNGESRSGGNR"
     gene            complement(5168..6175)
                     /locus_tag="PLC06_08040"
     CDS             complement(5168..6175)
                     /locus_tag="PLC06_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO90432.1"
                     /translation="MDDETSMRFLLSKQLKRAGYETATAADGEAAIAAANTTPFDAIV
                     LDVVMPGIDGFEVCRRLKADPRTVDVPVLFLSASSSGEFRRRAFSVGAADFLAKPYQI
                     ELLPDFLQATLRRADVTGPTTGRVVSIIGSSRPADAATVATRLAETTALKESGAVMLI
                     DLELPAGSIGARLQLSGGPNMRVLLQDTGEPVSDESIDRVAQRYHGALEVMPAPFSPS
                     FIGQSEPLPHRLNDVLDNLVARGYTVVVHLGSRINDLALTALHRSETILTVSTDGAKA
                     EHKALLDQLAAAGLHERVQQQADKTMPAPALPAQGAGQAHGQRQTRVKKQAPNRLPVA
                     A"
     gene            6397..7185
                     /locus_tag="PLC06_08045"
     CDS             6397..7185
                     /locus_tag="PLC06_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRO90433.1"
                     /translation="MNKEVELEVRADGIALLRVNRPEARNALSWAAQEAFADCVTAAN
                     HNDAIRVLIIAGANGSFVSGGDLKELSGHPDKAAGERLNRVMSAALAQLTDLPCPVIA
                     AVDGDAVGGGCEILTACDLRVASADARFAFRQIHNGLTTGWGGTSRLVALIGQSRAME
                     LLLTGRTFDAAEAQALGLIHRVVPAGTGALDAAYAWAEELRQLPRHALAALKTLVQAA
                     TYLPSLSANRLETQLFTRLWPAADHIEAMDAFVQKRAPRFNQDL"
     gene            7188..7964
                     /locus_tag="PLC06_08050"
     CDS             7188..7964
                     /locus_tag="PLC06_08050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90434.1"
                     /translation="MYDHIARYYDLSHDPLTDDIRFILDLAATVAGPVLEIGCGSGRL
                     LMPLARAGFDVVGVDASPEMLARARLRLTAEPPEVRARIRLIEADVRDLGLSPTESFG
                     LILFGYNTFMHFDEAGAGAALKRLRPLLRAAGRLLIDVVNPVLLSMATDDPDFMLEDV
                     LEDEVHGETIRQYTAYESVMGEQAVDVAWVYEVESAAGASGDGPKQTKARMRYFYLYP
                     HQYDLLLTLTGFRLVNLLGDYGSEPFDEDSDRLILLAAPE"
     gene            complement(7990..>8716)
                     /locus_tag="PLC06_08055"
     CDS             complement(7990..>8716)
                     /locus_tag="PLC06_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HRO90435.1"
                     /translation="PDFQRPDGNYETWYIRDAETQNYLSGFADWGRVEGRLLRYLIQG
                     PLFWLGMVELSAAGSDEATYRLSTRALAWLGDEPAVADEIKVPLIVQPDGIILAPFNA
                     GRYERFQAARIADPEPLTPGKPYRYRIVPSSLTEAQSQGISPERMLQFLESAGGRPVP
                     AAVRRGISRWAEKGVEGRLQSVVVLRVGDAEILDTLRANPKTRDFIAEALGDLAVVIR
                     RGEWEAFRQAVAQLGLLLDVEEL"
BASE COUNT         1747 a         2496 c         2736 g         1737 t
ORIGIN      
        1 cggcccggcc ccatgatgtg gctttggccg acgaattcgt ccagcgtgcg cggccgcatc
       61 cgggcagcca gcggcgcttc ctgctgaact cgctgccggg cggcgtggga gaagagatcg
      121 ctcattcagt gccgctctcc tggaacgcct tccgcaaaat atcttcaacg ccgagattcg
      181 cggcgttctc gcgcagatac aggatatcca gttgcccttt ttgcgtcaaa agcacaccga
      241 ggatgtcgtg ccattgacgt tccgaaagtc gccccccgcg gtcgaaccaa tcgagcttgg
      301 ccagaacaat gtcctcagga gtcgatagat agatcctctg gtcgccgtcc agcccgatcg
      361 ccgacgatac ccgccgctgg agttggagat gatcaaaggg gcgcgtgccg gggataaaaa
      421 catcgacctt gaacatcgtc ggcaggtgaa tgagattgaa actgctctta tgctcgatcg
      481 cgccccggat catttgttca tcaaggtaga aatcgcgccc cagggtttta accagcgccg
      541 ggacgtgctc cggcttgatg tcggccacga aatcaacatc gagggtggag cgactaactc
      601 cgtagtaggc agaggccatt gagccgccga tgacatactc aatcccgagg tcgttcaacg
      661 tatcggcgac ggtcgtcatg gtatcgatcg gggttatcga catggaatca ttctcccgta
      721 actggcgaat gtcgtaagtt atggtggtgc cgtatagcag accaaacaat cgtctggttt
      781 gttgttcggg cgagtcttgc gggtattgat ggcgtaatcc ggcaagcatc atctgccgca
      841 cgccatcgtt caactgaact accatctcca gcctgcgcca gggcgaaacc tgactgagcc
      901 gatagatgag ttcatcctcc acaatttggg acgtatccgc gaatagacga ctcatatcgt
      961 gattataccg cctaatggtt ggccggtcag cgaccggaaa cggcgagcca catcgccggc
     1021 tgtcaaaaag agccgatgtt gatcctctct gaatgtgtta ccattggttc gtacatccga
     1081 ttctgttaat agcaataatt atgacgctcg tgtatatggg gtgaatcgat gaaactggct
     1141 gaagcactca tcctgcgcgc cgatcacaag aagcggctgg aactcctgcg ggagcgacta
     1201 tcgcgcgtcg tcaaagtgca ggaaggcgac ttgccggccg aagacccggc cgacttgctg
     1261 ggcgaggtag acaggacggt gaccgaactg acgggcctca tccgtcgaat caatcgcacc
     1321 aacatggcgg ttcaacttga tgacggccga tcgattgccg acgccatagc cgaccgcgat
     1381 gatttgaagc tccgccattc gatcctcacc ggattgatcc agaacgcgac tatccggcag
     1441 gaccgattca ccaaaagcga agtgcgcttt caaacgacaa tcgatgtggc cgctctgcag
     1501 cggcgagcgg acgatctggc gcaggcttac cgcgaactgg acacgaaaat acaggcagcg
     1561 aattggctgt tcgatctgag cgaatgagtg aagttggtaa ccaggccgat taaggcgagt
     1621 acaaccctcc tgaaggcggg gaaagccttc accatggatg acgtgaaagg ccacgtctgt
     1681 ggttcgatcc cacgctaacc gggtatgccc cgtaatgtta caggcgtatc gggtaaagcg
     1741 taatgtcact accaacgtac tgattgatcg gcatggcgag ggtgggacag gggactgtca
     1801 ctcattgtga tgatcctgac caatcgggtg cgaacaatcg gccgctgttc gccccgttgt
     1861 atctctattt atcaagtggc cgctaaacaa aaaatgatgg aggtagtaga tggcaattca
     1921 agttaagcga gcgatgcttg atgtcaagaa atatcagtgg acggcagggg aaggggacga
     1981 caagaagagc gtcgcccatg accgaatcat gttcagccgc cgccaggggt atgaagtgat
     2041 tccgatgatc cagaaggtcg tcaatcattt tggttatgag accgaggaag atgtgaagcg
     2101 cgtcgaggaa gcgattcgct ccgcgctgcc cggcaacgtt cgcagccgca agaacgtctt
     2161 caattggctg accacctatc tcgcgtaaca ggcgggcaat cgctggaccg agcaaaaagg
     2221 ccggagaacc cttgggtgtc tccggccttt cgcgttcaat caggcgcgcc gcttcagctg
     2281 tgatgagacg tgttcgcggc catgctatcg tgcatggcgt acaggaagac gcccgccgca
     2341 accgccaggt tgagcgaact ggcccgacca tgcatgggca agcgcatcag gacgtcgcac
     2401 gcggccgttt gctccgcggt aagcccctcg cgctcgctgc ccagcagcag gacgaccggc
     2461 ggggtataga ggccggtatc ccgatagttg tcgcggccgt gggccgacgt gccggcgagg
     2521 tgatatccct cgcgcctgac ccaatggatc aattggctcc atgtggccgt cgcgaccggt
     2581 attcggaaga tcgcgcccat gccggcgcgg atggcggaag ggtgataggg atccacgccg
     2641 ccatcgacca gcatcagacc ggaagcgcct acggcgtcga tcgtgcgcag gatcgtgccg
     2701 acattcccag ggtcttgggg agcaatcagg gcgatcatcc aggggtgagt ggcggccgac
     2761 agatcctcga gccgggcaag acgctgccgg gcgacggcca gcagtccctg cggattatcc
     2821 ttgcccgcga cgctctccat cacttccgtc gtcactgcgt aagtcgggat ggcgctgctt
     2881 tccgccctgg ccacgagttc gcggccgaat ggactggcta atatgtcggg cgcgtagatc
     2941 agatattcgg ccgcgcccga cgccagcgct tcacccacat gaaagatgcc ctcgaccaca
     3001 cacagaccgg cggcctcgcg ttccttgcgt cgcccaaggg cgcgaacctg tttgatccgt
     3061 gcgttggtgg tgctggagat gagaggaggt gcggtcatga ttgcgcgggg caaaaagcgc
     3121 tgtcggcggg ctgctgcggc tagatctgaa accccccgcg ataggggaca accttgtaat
     3181 ggagatctgt gacgtatttt tccaactggt cgttaagctc atcccaggcg tcgctggtga
     3241 gtaggttatc catcgtctgc ttgtcgccgc agacaaagcc aagcaggcgg ttgggggcct
     3301 gaccataggc cgtatgccag gcctcgctca tttgcaggcc catggattgc aaagtgggca
     3361 caaattgacc cataacgaac tggtaatatt cctggagggt gtcctggttc acatcatatt
     3421 cgagtaataa cttcagcccg ttctcgtcca tagacattat tgtaacgcca catcgacgta
     3481 aaacgacagg cgatgaaaaa ttttaatcaa gacagaccga ttggcgactt aatcggggat
     3541 gagttgcagg cgccatattt acgaccatcg ggcaagtggt gtacaatgaa cgaaagtggc
     3601 ctgagcgatc gctcgtcccc aaggcccagg agaacggctt atgcatagtt gcaggagttc
     3661 gctgaattgc ttcgtccccc catacatgct gcgcgagatc attgagaacg gcacaccgga
     3721 gcagcgccag caggctttgc ggaacatcgt gtcggccgaa gccgttcggg tgcagcgcga
     3781 agtgatcgcc aaggaaggcc cgcctgcgcc attcctggaa gccgcgggtg gcttgaaccg
     3841 cgtcatctat accgccgaga acggcagcac cttgcccggc cgccagttgc gcggcgaagg
     3901 cggcgcgccc accggcgacc ccgctgccga tgaggcctac gacggtgcgg gcgcgacgca
     3961 cgcgctcttc tatgaggtgt tcgagcgtga ttccatcgac ggcgcgggca tgcgtctcga
     4021 ctccacagtg cattatcgta ccggctacga taacgccttc tggaacgggc ggcagatggt
     4081 gtatggtgat ggagacgaag atctgccgga gagccagcgc atcttcaatc gcttcaccat
     4141 cgccgtcgat attatcggtc atgaattgac ccacggcgtg acccagcata cggccaacct
     4201 gatctatcag ggccaatcgg gcgcgctcaa tgaatcgata tcggatgtgt tcggctcgct
     4261 ggtcaagcag cggctattgg gccactcggc ggccgaagcg gactggatta tcggcgaagg
     4321 actgttcacg tcaaacgtca acggcatcgg catccgcagc atgaaggctc cgggcacggc
     4381 ttacaatgat cctgtgctgg gcaaggaccc tcagccgggg catatgcgcg actacgttca
     4441 gacgcagtcc gataacggcg gcgtccatat caattccggc ataccgaacc gcgcgttcca
     4501 cgtgacggcc atgaatatgg gaggctacgc atgggagaag gcggggctta tctggtatgc
     4561 cacgctgaag gataagttga ctgaatcggc cacgttcgcc acggcggcgg ccaagactta
     4621 tgaggctgcc cgcgacctgt atggccaggg cagtctggag cagcaagctg tgcgcgccgg
     4681 ttgggccgag gtcggcattg atgtcggcca ggaaacgccg cccccccctg ataatggcgg
     4741 ctcccgtcct ggttgttcgg gcagcctgat gagcctgttc cgatcgatca taggcgcgcg
     4801 ctagaggaaa attgcgagag aggtccgaat gaaaatctac tttgagcgaa gcggcggctt
     4861 tatggggcgc aacgtatcta ctacggtgga taccaatcag atcccgccgg agcaagcctt
     4921 gagcttgctg aaaaaggtgg attactctga ttttttctgc ctgccggaat cgttgaccgg
     4981 cggactggag agcgcgtccg gcgcggacca attgtgctac aaggttactg tcgacgtgga
     5041 cggcatcgag cacaccgtgg agacgtcgga tgccggcgcg ccggcagagt tgcagccatt
     5101 gctggacgag ttgtcctcaa ttgctcgcgg ctcaactatc cccacggggc caaacggcga
     5161 atcacgatca ggcggcaacc ggtagccggt tgggcgcctg tttcttgacg cgcgtctgtc
     5221 gttggccgtg ggcctgtcct gccccctgag cgggaagggc cggagcgggc atcgttttgt
     5281 ctgcctgctg ctggacgcgc tcgtggagtc cggctgccgc caactgatcc agcaacgcct
     5341 tgtgctctgc cttcgctcca tcggtcgaca cggtcaggat cgtttccgag cgatgcaatg
     5401 ccgtcagggc cagatcgttg atccggctgc ccaggtgaac cacaacggta tagccccgcg
     5461 cgacaaggtt gtccagcacg tcattgaggc ggtgggggag aggttcgctc tgcccgatga
     5521 atgatgggga aaacggcgcg ggcattactt ccagcgcgcc gtggtagcgt tgggccacgc
     5581 ggtcgattga ttcgtcggag acaggctctc cggtatcctg cagcaaaaca cgcatgtttg
     5641 gtccgccgga aagttgaaga cgagcaccaa tgctgcccgc gggcagttcc aggtcgatga
     5701 gcatgaccgc gccggattct ttcagggcgg ttgtctcggc cagtcgtgtg gcgaccgtgg
     5761 ctgcgtcggc cggccgggag gagccaatga tcgagacgac gcggccggtc gttgggccgg
     5821 ttacatcagc gcgacgcagg gtggcctgga ggaagtcagg gagcaactcg atctggtatg
     5881 gtttggccag gaaatcggcc gcgccgacgc taaatgcacg ccgccggaac tcaccgctgg
     5941 atgaggccga aagaaacagc acgggtacat ctaccgtgcg cgggtcggcc ttcagccgac
     6001 ggcacacttc gaagccgtca atgcccggca tgacgacgtc gaggacgata gcgtcgaatg
     6061 gtgtggtgtt cgctgccgcg atggcagctt cgccgtcggc ggccgtggcc gtctcgtaac
     6121 cggcgcgttt tagttgtttg ctcagcaaaa agcgcatgga cgtttcgtcg tccacgataa
     6181 gaattcgttt ttgattcatg cctgttaaat gattcctggg ttaccgcgtt ctgacagcag
     6241 agattccggc ggtttagtgt ctacccagga gattagcggc tcccggttac aatgaacctt
     6301 aagaggatag cttcattgga ggcagaaggt cgtatatcgc ttgtctcgat gcgcgcgcag
     6361 atgcgtctcg ccctgatttc tgctatcgta ctgcctatga acaaagaggt tgaacttgag
     6421 gtccgcgcgg atggtatcgc gctgttgcgc gtgaaccggc ccgaggccag aaatgccctg
     6481 agttgggccg cccaggaagc tttcgccgac tgtgttacgg ccgccaacca taacgacgcg
     6541 atccgcgttc tcattattgc cggcgcgaac gggtcattcg tatccggcgg cgatttgaag
     6601 gagttgtccg ggcatcctga caaggctgcc ggagagcggc tgaatcgggt gatgagcgcc
     6661 gcgctggccc aactgacgga cctgccctgt ccggttatcg ccgctgtgga tggggatgcc
     6721 gtcggtggcg gctgcgaaat tctgaccgct tgcgatttac gagtggcctc ggccgacgcc
     6781 cgcttcgctt tccgacagat acacaatggg ctgacgaccg gctggggcgg cacgtcgcgg
     6841 ctggtggcgc tgatcggcca aagtcgggcg atggagctat tgcttacggg ccggacgttt
     6901 gatgcggctg aagcgcaggc gctgggcctc atccatcgtg tcgtgccggc gggcaccggc
     6961 gcgctcgacg cggcttatgc ctgggccgag gagttgcgac agctgcctcg ccatgccctg
     7021 gccgcgctca agactctggt tcaagccgcg acctatctcc cttcgctaag cgcgaaccgg
     7081 ctggagacac agctgttcac ccgtttgtgg ccggcggccg accacattga ggccatggac
     7141 gcctttgtgc agaagcgcgc gccgcgtttt aatcaagacc tgtagccatg tacgaccaca
     7201 tcgcgcgcta ttacgatctg agtcacgatc cgttgaccga cgacatccgg tttatcctcg
     7261 atctggccgc aaccgtcgcc gggccggtgc tggagattgg ctgcggcagc gggcggttgt
     7321 tgatgccgtt ggcgcgggca ggctttgacg tcgtcggtgt tgatgcgtcg ccggaaatgc
     7381 tggctcgcgc cagattacgt ctgacggctg aaccgccgga agtgcgcgcc agaattcggc
     7441 tcatcgaggc cgacgtcagg gatctgggcc tttctccgac ggagtcgttc gggctgatcc
     7501 tcttcggcta taacacgttc atgcatttcg atgaggccgg ggcgggggcg gcgcttaagc
     7561 gtttgcggcc gttgttgcgc gccgcaggac ggctgctcat cgacgtggtg aaccctgttt
     7621 tgctgtcaat ggcgaccgat gaccccgatt tcatgctcga ggacgtgctg gaagacgagg
     7681 tacacggaga aacgatccgt caatataccg cttatgaaag cgttatgggc gagcaagctg
     7741 tcgacgtggc ctgggtatac gaggttgaaa gcgcggcggg ggcttccggg gatgggccga
     7801 aacaaaccaa agcccggatg cgctacttct acctatatcc acaccagtat gatctgttgt
     7861 tgaccctgac cggctttcgc cttgtgaact tgcttgggga ttatgggagc gagccgttcg
     7921 acgaggacag cgatcggctt atcctgctgg ctgcgccgga gtgaccacag gcggccgcca
     7981 ccggcgacac tacagctctt ccacatccaa taacagcccc agttgggcta cggcctggcg
     8041 gaacgcttcc cattctcctc gccggataac cacggccaga tcgcccaacg cctcggcgat
     8101 gaaatcgcgc gtcttgggat tggcccgcaa tgtgtcgagg atctccgcgt cgccaacgcg
     8161 cagcaccacg accgattgca gccgtccctc gacgcccttt tccgcccaac ggcttattcc
     8221 tcgccggacg gcggccggga cgggacgacc gccggcgctt tccaggaatt gcagcatcct
     8281 ttcgggtgag atgccctggc tttgtgcttc ggtcagcgat gaggggacga tgcggtagcg
     8341 gtagggcttg ccgggagtca gaggctccgg gtcggcgatg cgggccgcct gaaaccgttc
     8401 gtagcgaccg gcgttgaaag gagccaggat gatgccgtcg ggctggacga tcagcggcac
     8461 cttgatctca tcggccaccg ccggttcatc gcccagccag gccagcgcgc gtgttgacag
     8521 gcgataggtg gcctcgtcgc tgcctgcggc cgataactcg accattccca gccaaaagag
     8581 cggcccctgg atcagatagc gcagcagtcg cccctcgacg cgcccccaat ccgcgaagcc
     8641 ggagagataa ttttgcgtct cggcatcgcg gatgtaccag gtctcgtagt tgccgtcggg
     8701 gcgctgaaaa tcgggg
//
LOCUS       DAOMQG010000116         8655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3499_length_8655_cov_6.528953, whole genome shotgun sequence.
ACCESSION   DAOMQG010000116 DAOMQG010000000
VERSION     DAOMQG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_8655_cov_6.528953"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..743
                     /locus_tag="PLC06_08060"
     CDS             216..743
                     /locus_tag="PLC06_08060"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRO90436.1"
                     /translation="MNSNFTKKIKGYVAAKVNGTRDEVRLNVREMLAAEAPAGELAGA
                     FAAGTLISTIPVPLVDMALAAWVTKRYEGMPRAPFFTGMALANNLFMAPLYASTPRVG
                     GFALHWMASHTPIVAPEAILIRIIVGYLLIAAGLALGAYVLASTGFRGYQATRRLRPA
                     GSAICRIASSSTQSL"
     gene            818..1276
                     /locus_tag="PLC06_08065"
     CDS             818..1276
                     /locus_tag="PLC06_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRO90437.1"
                     /translation="MTTHYTGTQEEKQALDAYIKLSRAAESVGKRINDHLNAHDLTVS
                     QFGALEALYHLGPMPVGQLAGKILRSSANLTLVIDNLVKRGLVTRQRRTDDRRAIDIH
                     LTDDGRDLIARILPHHVAGVVCAFAPLSAEEQAALAALCRKLGKAQGNQE"
     gene            1336..2289
                     /locus_tag="PLC06_08070"
     CDS             1336..2289
                     /locus_tag="PLC06_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HRO90438.1"
                     /translation="MKPIQGLHHITAMASDPQANLAFYHNVLGQRLVKRTVNFDDPGT
                     WHFYFADETGTPGTVMTFFPWPRAKRGTLGNGETAAVAYAIPSGSVDYWRERLAAHGV
                     GVSDGGTRFGDPVLAFHDPDGMRLELIATDAPATIRHWHDGPVPAAHALRGFHSVTLW
                     QDEIEPTADLLTTHMGYTLTGQEGDRTRLSAASNDMGLHVDLLYRPNQPGGRFGAGSV
                     HHIAFRTVDDSEQQEYLDALRRAGFGVSPVRDRQYFHSIYFRSPGGVLFEVATDAPGF
                     LYDEPVETLGEALKLPAWYEPQRAEIEAVLPPITLPQAEAQ"
     gene            2292..2945
                     /locus_tag="PLC06_08075"
     CDS             2292..2945
                     /locus_tag="PLC06_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRO90439.1"
                     /translation="MADRIHQGLPVAAAGKPLSEAGGALILIHGRGASAESILDLADF
                     LPRPDLAYLAPQAADSTWYPYSFLSPLEKNEPYLSSALARVGETVAMAEAAGLPPERI
                     FLGGFSQGACLASEFVARNARRYGGLIVFSGGLIGPEGAPRDYRGTLSGMPAFIGCSD
                     VDPHIPVGRVRETAAVLERMGAAVDCRIYPRMGHTINQDEIDAAASLLTAAMKSAGV"
     gene            complement(2970..3356)
                     /locus_tag="PLC06_08080"
     CDS             complement(2970..3356)
                     /locus_tag="PLC06_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90440.1"
                     /translation="MSLPVDETAILATGSRTLALGLSALLLSIPPIHHVKQVPDADAL
                     FDAIEGSKPILIIVDTALLEGHLPDAWQAICRLAPTAHYVLLSDNTIETAKSVHCDGA
                     MVVMQGTDPRQLAHDIELLLSGHAVA"
     gene            complement(3528..4190)
                     /locus_tag="PLC06_08085"
     CDS             complement(3528..4190)
                     /locus_tag="PLC06_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO90441.1"
                     /translation="MGNEIRILICDDHAVVREGLRALISTEPDMIIAGEAVNGENAVA
                     AFRELRPDVTLLDMVMPRMDGVEALKIIVSEFPGARVLVLTSFSDDEMVFPAIKAGAL
                     GYLLKDSTPEELVRGIRAVNRGEASLHPSIARRLIQELSRPATLPPTQEPLTEREMEV
                     LRLVAKGYSNEEIGDSLVVSERTARGHVSSILSKLHLANRTQAALYALREGFADLEDQ
                     SE"
     gene            complement(4159..5970)
                     /locus_tag="PLC06_08090"
     CDS             complement(4159..5970)
                     /locus_tag="PLC06_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRO90442.1"
                     /translation="MQPSPFDPGTLHTRLFHSGPVAMAITNPADGRFLDVNAAFANLV
                     AVPSADLLADGDVAEQQFQFRLDGFPEDQALPREPAARSEGQLCTARGEFHEVLVSTQ
                     LVEWDGRQFLMHIIQDMTAIKQTEIVVRASQARFRLYFNAVPLPVFVYDSQTLKILDL
                     NPAAVSQYGYTREELLALTMLDIRPPDERTKYLGLVQTLSPDMARFGIWIHQKKDSTT
                     MPVDVTSYALELGGRVVRLAVCRDVGEQLRMEAALRASEERHRIIAEVTNDVLWDINF
                     ATNGIVFSEGMAGVFGYTLTREEPVEWWLHHIHPAEREAVRVKFDAAIAGSDVDWTDT
                     YRFLRRDGSYAHVRDRGHIFRDKDGAPARMVGAMIDITRQIEVQEAATQAALEERRRL
                     ARDLHDAITQSLYSLSLLAEVARRRAEQGNMAATTEGINRLGDLAQQSLKEMRLLVHE
                     LRPSLLQKEGLVTALQARLDTVERHSGVRARLVAQLDQTLSPQVQLHYYLVAEEALNN
                     ALKHAAASAVEVHLRSVHGAAANTLVMEISDNGKGFDPATINASGGMGLTNMRERMET
                     LGGRLEVDSSPEKGTTIRVYVDIGDENHGKRNSDTDL"
     gene            complement(6042..7820)
                     /locus_tag="PLC06_08095"
     CDS             complement(6042..7820)
                     /locus_tag="PLC06_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRO90443.1"
                     /translation="MAALFGGLITTINPAEQCTLFSQVFDGSPVGMGILSAAGNTYID
                     ANETLAALLGYSRDELRGLPTDDVGVAIDLDAQLLLETLREYREIGGIPITVTNRDGH
                     DHTCVLSSQLVEIDGEEYIFFLFHEYTPEEKPRTALAQFETRFKLFYQGFPLPLLVID
                     DESARIADVNPAAIDLYGYDRDEFLGLSWDSLVSEGDRPGLAAQPAAALSGIMCHRLR
                     DGRLIDANVSSFSFRLDDRPATLSIVEDITEQQAVRAALEASEERQRIIADMTADAIR
                     DRDLITGKVVWNLGLRDLFGYDTHDPAGRSWWSERIHPDDRAAVLATLDAALRSDVGD
                     WMGEYRFRRADGAYANVLDNTHIMRDAGGRPTRLIGSMVDITEQLKLADVAAKAALEE
                     RQRLAHTLHDSVSQSLYSISLLAEASRRLALAGEQQGVTDYVARLGELTIQTLRQMRL
                     LLYDLRPGVLDQEGLSGALRHRLEAVEHRAGIRARLIDHSLGPVSPALQREIFWIAQE
                     ALNNSLTHASATAVNVTLSSTNDEIVLEVSDNGQGFDPNTTAEDGGLVAIDHRVSELG
                     GTLAVNSGADRGTMLIVRVPTAPMPG"
     gene            complement(7856..8221)
                     /locus_tag="PLC06_08100"
     CDS             complement(7856..8221)
                     /locus_tag="PLC06_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90444.1"
                     /translation="MLRRTKLQRVETIDRLRAENLKKYTEVYTKDDERIGVTLRFVHR
                     PIEDVNEELRLYRSYLVLQSIVLGGPAYVPTVYVGTYDPLGNRLNLTADMNTLEDETW
                     NREPDFVARGQGVYEELPE"
BASE COUNT         1575 a         2765 c         2736 g         1579 t
ORIGIN      
        1 gtgcatcagt acttgcgaac tgtctaggcc cacatgcatc cgggggcggg agtgtcacac
       61 gctcccgccc ccggattacc cccattccta cgccaacctg acgcgctccc catgccacac
      121 ccacgggagg cataaaccgg gaaccccacg cgggcgttca tcgttttcca ctcacaaggc
      181 cgaacgaacc caatttcggc ggccggagga gaacgatgaa cagcaatttc acgaagaaga
      241 tcaagggtta tgtggcggcg aaggtcaatg gcacgcgcga cgaagtgcgc ctcaatgtgc
      301 gggagatgct ggccgccgaa gcgcccgccg gcgagctggc cggggcgttc gccgcgggca
      361 cgctcatcag caccatcccc gtgccgctgg tcgatatggc cctggccgcc tgggtgacca
      421 agcgctatga agggatgccg cgcgcgccgt tcttcaccgg catggccctg gccaacaacc
      481 tgttcatggc tcctctctat gcgtctacgc cccgggtggg cggtttcgct ctccattgga
      541 tggccagcca cacgcccatc gtcgccccgg aagcgatcct catccgcatc atcgtcggct
      601 atctgctcat cgcggcgggg ctggcgctgg gagcctacgt gctggcctcc accggcttcc
      661 gtggctatca ggcgacgcgc cggctgcggc ctgccggtag cgcaatctgc cggattgcct
      721 cttcgtccac tcaatctcta tagcccactc tcttgacaac cgtgccccat catgatatta
      781 tattgatatc aaattatatt atatcaatat atttatcatg acgactcact acacaggcac
      841 gcaagaagaa aaacaggcgc tggatgccta catcaagctc agccgggcgg ccgaatcggt
      901 cggcaagcgc atcaatgacc atctcaacgc ccacgacctg acggtcagcc agttcggggc
      961 gctggaggcg ctctatcatc tggggccgat gcccgtgggc caactggccg gcaagatctt
     1021 gcgcagcagc gccaatctga cgctggtcat cgacaacctg gtgaagcgcg gcctggtgac
     1081 gcggcagcgg cgcaccgacg accggcgcgc catcgacatc catctcaccg acgacggccg
     1141 cgacctgatc gcccgtatcc tgccccacca tgtagccggg gtcgtctgtg ccttcgctcc
     1201 cctctcggcc gaggagcagg cggcgctcgc cgcgctctgc cgcaagctgg gaaaagcgca
     1261 agggaatcag gaatgatgaa tgaagagcgc gcttttcatt cgtcattcgt cactcaaaaa
     1321 aggaactaca tcacaatgaa acccatccaa ggcttacatc acatcaccgc catggcgagt
     1381 gatccccagg ccaacctggc gttctaccac aacgtgctgg gccagcgact ggtcaagcgg
     1441 acagtcaact tcgacgatcc cggcacctgg cacttctact ttgccgacga gaccggcaca
     1501 ccgggcacgg tgatgacctt cttcccctgg ccgcgcgcca aacggggcac gctgggcaac
     1561 ggcgaaaccg cggccgtggc ctatgccatc ccgtccggct ccgtcgatta ctggcgcgag
     1621 cggctggccg cccatggcgt gggcgtgtcc gacggcggca cccgcttcgg cgatccggtg
     1681 ctggccttcc acgaccccga cggcatgcgc ctggagctga tcgccaccga cgccccggcg
     1741 accatccgcc actggcacga cggccccgtc cccgccgccc acgccctgcg cggcttccac
     1801 agcgtcacgc tgtggcagga cgagatcgag ccgacggccg acctgctgac cacccacatg
     1861 ggctacacgc tcaccgggca ggaaggcgac cgcacccgcc tgtcggccgc gtccaacgac
     1921 atggggctgc acgtagacct gctctatcgc cccaaccagc cgggcggccg cttcggcgcg
     1981 ggttccgtcc accacatcgc cttccgcacc gtcgacgaca gcgagcagca ggagtatctc
     2041 gacgcgctgc gccgcgccgg attcggcgtg tcgcccgtgc gcgaccggca atactttcac
     2101 tcgatctatt ttcgctcgcc gggcggggtt ctgttcgagg tggccaccga cgcgccggga
     2161 ttcctctatg acgagccggt cgaaacgttg ggtgaggcgc tgaagctgcc cgcctggtac
     2221 gagccgcagc gggcggagat cgaggccgtt ttgccgccaa tcaccctgcc gcaggcggag
     2281 gcgcagtaag aatggccgac cggattcatc agggactgcc tgtcgccgcc gccggcaagc
     2341 cgctcagcga agccggcggc gcgctgatcc tcatccacgg ccgcggcgcg tcggccgaga
     2401 gcatcctcga tctggccgac ttcctgcccc ggcccgatct ggcctacctg gccccacagg
     2461 cggccgattc gacctggtac ccctactcct tcctgtcccc gttggagaag aacgagccgt
     2521 atctgtcctc ggcattggcc cgcgtgggcg agacggtagc gatggcggag gcggcggggc
     2581 tgccgccgga gcgcatcttc ctgggcggat tctcgcaggg ggcgtgtctg gcgagcgagt
     2641 ttgtggcccg caatgcccgg cgctatggcg ggctgatcgt ctttagcggc gggctgatcg
     2701 ggccggaagg cgcgccgcgc gactatcgcg gcaccctgtc gggcatgccc gcgttcatcg
     2761 gctgcagcga cgtcgatccc cacatcccgg tcggccgcgt gcgcgagacg gcggcggtgc
     2821 tggagcgcat gggggcggcg gtcgattgcc gcatctaccc gcgcatgggc cacaccatca
     2881 atcaggatga gatcgacgcc gccgcgtctc tgctgacggc ggcgatgaag agcgccgggg
     2941 tctgacaggt cagggcctgg ggcggcgagt caggcgacgg cgtggccgct gaggagaagc
     3001 tcgatgtcgt gagccagttg gcgggggtcg gtgccctgca tgaccaccat ggccccatcg
     3061 caatgaacgc ttttggcggt ctcgatcgtg ttgtcgctca gcaagacgta atgcgctgtg
     3121 ggggccagac ggcaaatggc ttgccaggca tcaggcaagt ggccttccaa cagcgctgtg
     3181 tcgacgatga tcaggattgg cttgctccct tcgatcgcgt cgaaaagcgc gtcggcgtcg
     3241 ggaacctgct tgacgtggtg gatgggcggg atggagagaa gcaaggccga taggccaagc
     3301 gccagggttc tcgatcccgt ggctaaaata gctgtttcat ctaccggtaa ggacatacgc
     3361 tattcagacc cccacctaaa gtgtaacgaa tttcctcttg gcaggaatag ggcttctgtc
     3421 gagaagatat gcggcggaat gatccgaatc ggcataaaga aagcgacgct gatgcgtcgc
     3481 ttatagtaca aattgcccag acaggatgaa gagttcaact tgtcgtctca ttcgctctgg
     3541 tcttcaagat cggcaaagcc ttcgcgcagg gcatagagcg ccgcctgagt acggttggcc
     3601 aaatgcagct tgctcaggat gctgctgacg tggccgcggg ccgtgcgctc gctgaccacc
     3661 agagagtcgc cgatctcctc gttggaatac cccttggcca ccaggcgcaa tacttccatt
     3721 tcccgctcgg tcagtggttc ctgggtgggg ggcagggtcg ccgggcgact cagctcctgg
     3781 atgaggcggc gggcgatgct ggggtgcagc gaggcctcgc cgcggttgac ggcgcgaatg
     3841 ccccgcacca gctcctcggg cgtcgaatcc ttgagcagat agcccaatgc gcctgctttg
     3901 atggccggaa agaccatctc atcgtcggag aatgaagtca gcaccaggac gcgcgcgccg
     3961 gggaactcgc tcacgatgat cttgagtgcc tcaacgccgt ccatgcgcgg cataaccatg
     4021 tcgagcaggg tgacgtcggg ccgcagttcg cggaaagcgg cgacggcatt ctcgccattc
     4081 accgcctcgc cggcgataat catgtccggt tcggtggaga tgagggcgcg aagcccttca
     4141 cgcaccacgg catggtcatc acagatcagt atccgaattt cgtttcccat gattttcgtc
     4201 cccaatgtcc acatagaccc gaattgttgt acctttttcg ggcgacgagt cgacctccag
     4261 ccggccgccc agcgtttcca tgcgctcgcg catgttggtc aggcccatcc cgcctgaggc
     4321 gttgatcgta gccggatcaa accccttgcc gttgtcggag atctccataa ccagtgtgtt
     4381 cgcggccgct ccgtgaacgg agcgcagatg aacctccacc gccgacgcgg ccgcgtgctt
     4441 cagggcatta ttgagcgcct cttccgccac cagataatag tgcaattgta cctgtgggga
     4501 caaggtttgg tctaattgtg ccacaagacg ggcacgaacg ccggaatggc gctccacagt
     4561 atcgaggcga gcctgcagtg cggtgaccag cccctctttc tgcaatagcg acggccgcag
     4621 ctcatgcacg agaaggcgca tctccttgag cgattgctgc gccagatcac ccagccggtt
     4681 gatgccctcg gtagtcgccg ccatattgcc ctgttcggcg cgccgccggg cgacctcggc
     4741 caacaacgac agactgtaga gcgattgggt gatggcgtcg tggagatcgc gcgccagccg
     4801 gcgacgttcc tccagggcgg cctgggtggc cgcttcctgc acctcgatct ggcgggtgat
     4861 gtcgatcatc gcgccgacca tccgcgccgg cgcgccgtcc ttgtcgcgaa agatgtggcc
     4921 ccggtcgcga acgtgagcat agctgccgtc gcggcgcaga aaacggtagg tgtcggtcca
     4981 gtccacgtcg ctgccggcga tggcggcgtc gaatttcacc ctgaccgctt cgcgctcggc
     5041 ggggtggatg tggtgaagcc accattcaac cggctcttcc cgagtgagcg tgtagccgaa
     5101 aacgccggcc atgccctcgg aaaaaacgat gccgttcgtc gcgaagttga tatcccacag
     5161 gacgtcgttg gtgacttcgg cgatgatgcg gtggcgctct tcgctggcgc gcagggccgc
     5221 ttccatgcgc aattgctcgc ccacatcccg gcagacggcc agccgcacga cgcggccgcc
     5281 caactccagc gcgtagctcg tcacatccac cggcatcgtc gtggaatctt ttttctgatg
     5341 aatccagatg ccgaaccggg ccatatcagg cgacagggtc tggacgaggc ccagatactt
     5401 cgtgcgctca tccggggggc gaatgtcgag catggtcagc gcgagcagct cctcgcgtgt
     5461 gtaaccatat tggctcaccg ccgccgggtt gaggtccaga attttcaggg tttgcgaatc
     5521 gtagacaaac accggcaaag gaacggcgtt gaaatacagc cggaaacggg cctgtgacgc
     5581 ccgcaccaca atttcggtct gcttgatggc cgtcatgtcc tggatgatgt gcatcaggaa
     5641 ctgccggccg tcccattcca ccaattgggt agagacgagc acctcatgga actcgccgcg
     5701 ggccgtgcac aattggcctt cgcttcgggc agccggctcc cgcggcagcg cctgatcttc
     5761 cgggaagccg tcgagccgga actgaaactg ttgctcggcc acgtcgccat cggccaacag
     5821 atccgccgaa ggaacagcca ccagattggc gaacgccgca ttgacgtcga ggaagcggcc
     5881 gtcggccggg ttggtaatgg ccatggccac agggccggag tggaagagcc gggtgtggag
     5941 tgtgccgggg tcgaacggtg atggctgcat gatggcgatg atgattatac cgttttgcgt
     6001 cgacgatccg aacgatagtt ttcagccttt tcaagggccg atcaccccgg catgggggca
     6061 gtcgggaccc ggacgatcaa catggtgccc cggtcggcgc cggagttgac ggccagcgta
     6121 ccgcccagtt cgctcacccg gtgatcgatg gcgactaacc cgccgtcttc ggccgtcgtg
     6181 ttggggtcga acccctggcc gttgtcgctg acttccagga cgatctcgtc attcgtcgag
     6241 gacagcgtga cgttgacggc cgtggccgag gcatgggtga gcgagttatt gagcgcttcc
     6301 tgggcgatcc agaatatctc acgttgcagc gccggggaga cggggccgag gctatggtcg
     6361 atcagtcgtg cccgaatacc cgcgcgatgc tcgacggctt ccagcctatg acgcagggcg
     6421 cccgacaaac cttcctgatc cagcacaccc ggtcgcagat catagagcag cagccgcatc
     6481 tggcgcaggg tctggatcgt cagctcgccc agccgggcaa cgtaatcggt aaccccctgc
     6541 tgttcgccgg ccaaagccag tcgccgcgag gcttcggcca gcagactgat ggagtagagc
     6601 gactggctca cggagtcgtg gagcgtgtgg gccaggcgct gccgttcctc cagcgcggcc
     6661 ttggcggcca cgtcggccag cttgagctgc tcggtgatgt ccaccatgct gccgatcagc
     6721 cgcgtgggcc ggccgccggc gtcgcgcatg atatgggtgt tgtccagcac gttggcgtag
     6781 gcaccatcgg cccgacggaa gcgatactcg cccatccagt cccccacatc cgacctgagg
     6841 gcagcatcca gcgtcgccag aacggcggcg cgatcgtcgg ggtggatccg ctcgctccac
     6901 cacgatcggc cggccgggtc gtgggtgtca tagccgaaca gatcgcgcag acccaggttc
     6961 cagactacct tgccggtgat cagatcgcgg tcgcggatgg cgtcggcggt catgtcggcg
     7021 atgattcgct gacgctcctc gctggcctcc agcgccgccc gcaccgcctg ctgctcggtg
     7081 atgtcctcga cgatcgacag ggtggcgggc cggtcgtcca gccggaacga aaagctggag
     7141 acattggcgt cgatgaggcg gccgtccctc aggcgatggc acatgatgcc cgacaaggcg
     7201 gccgcgggct gcgcggcgag accggggcga tcaccctccg acacgaggct atcccacgac
     7261 aagcccagaa actcgtcccg gtcatagcca tagagatcga tcgccgccgg gttcacgtcg
     7321 gcaatgcgcg cgctttcgtc gtcgatgacc agcagcggca ggggaaagcc ctggtagaac
     7381 agcttgaagc gggtttcaaa ttgggccagg gccgtccgcg gcttctcctc gggggtgtat
     7441 tcatgaaaaa ggaagaagat atactcttcg ccgtcaatct ccaccagttg cgacgacagc
     7501 acgcaggtgt ggtcgtgccc gtcgcggttg gtgacggtga tgggaatgcc gccaatctcc
     7561 cggtattccc gcagcgtttc gaggagcagc tgcgcgtcga ggtcaatggc cacgccgacg
     7621 tcatcggtgg gcaggccgcg caattcgtcg cggctgtagc cgagaagagc ggccagggtc
     7681 tcgttggcgt cgatataggt gttgccggcg gccgacagga tgcccatgcc caccgggctg
     7741 ccgtcgaaaa cctgactaaa gagggtgcat tgctcagccg ggttgatggt tgtaatcagg
     7801 cctccgaaaa gggcagccac ccgggcctgc ccgtgccgtc actcgttgcc cgcggttact
     7861 cgggcaactc ttcgtagacg ccctgcccgc gagccacaaa atccggttcg cggttccagg
     7921 tttcatcttc cagcgtgttc atatcggcgg tcaggttcag ccggttgccc agcggatcat
     7981 aggtgccgac atagaccgtt ggaacgtaag ccggcccgcc cagcacgatg ctctgcagga
     8041 cgagatagga gcgatagagg cgcagctcct cgttcacatc ttcgattggt cggtggacga
     8101 aacgcagggt gacgccgatg cgctcgtcgt ctttggtata cacctcggtg tatttcttga
     8161 ggttttcggc ccgtagcctg tcgatggttt cgacccgttg aagttttgtc cgtcgcagca
     8221 tatcgcgctc ctgtaagacg acctggaaat aatagcggca gggatgataa gcctattgta
     8281 caggagagca ggaagaggca caagcagcgg gaatcagatc gcaggctttt tcagttttcg
     8341 attgacgcga ccaaacgggt aggggcgtgg cagcggcgaa atcatctacg tcttgcagaa
     8401 cggtcttgtc tcgccgctgc ctcgccctct tcgcccggag ggtcaggcaa ccgggaagga
     8461 aggccgcgcg tttgccggcg acggccgtcc cggttgcctg acccctacgc ctcacgatcg
     8521 gcatggatca ccctacagcc tcgactactg aaaaagcctg tggtcagtgg gcagtggtca
     8581 gttcactgac cactgcccac taactcttca tctgcgaagt cacggctgga gacatccgag
     8641 cgaaatccgc gggtt
//
LOCUS       DAOMQG010000117         8617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3519_length_8617_cov_8.711866, whole genome shotgun sequence.
ACCESSION   DAOMQG010000117 DAOMQG010000000
VERSION     DAOMQG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3519_length_8617_cov_8.711866"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLC06_08105"
     CDS             <1..348
                     /locus_tag="PLC06_08105"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRO90445.1"
                     /translation="VILSQTLQFLNDPMMILGEMLRVGRLAVVSFPNFGFWRCRAHLL
                     VYGKVPQAPDYPQAWHDTPRQQAFTIADFTAFCRENSVEVRQETYLSGERHVTQWSNL
                     LARTAVFALERQI"
     gene            complement(413..778)
                     /locus_tag="PLC06_08110"
     CDS             complement(413..778)
                     /locus_tag="PLC06_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRO90446.1"
                     /translation="MRLILRILINAVALGLTFWIVDLCWDGSILPLLIIALIFGVVNA
                     LIRPLVTILTCPLVVLTLGLFLFIINGLMLWLTIWLSRAIGIGFTCMPDFFWNIVIGA
                     VVLTIISGVATALLPDDWE"
     gene            935..2668
                     /locus_tag="PLC06_08115"
     CDS             935..2668
                     /locus_tag="PLC06_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRO90447.1"
                     /translation="MDAILTQAQETLLKRTRDALGRLRDVLNADAINPAGHAALVESI
                     RQLDELFLLVIAGEFNSGKSAFINALLGQKLQPEGVTPTTDQIYLLRYGETQQRVPGE
                     NGVWIQTAPVELLGKLTVVDTPGTNAIVREHEALTSEFIPRSDLVLFVTSADRPFTES
                     ERAFLEQIRNWGKKIVLVINKIDILGSEADVDQVIAFVTNSARSLIGDVAAVFPVSAR
                     LAQAAKNGQPDKWTPSRFEPLETYIRETLDDAGRFRLKLLNPLGVGARLTREQLADLN
                     ADLSTLTEDSKLLDDIRGQTVYYDEDMQRNFKARIAEIDGLLLGMEKRGLTFFDDRLR
                     LGRIPDLLRSKQLEQDFQQEVVADTPRQIEDRVNELVDWMVQQDLRQWTAVADHLGRK
                     RDVHQGRIVGDAPREGTLAYDRQRLIDSIGRATREAVNSYDETREAAELAASARQAVV
                     NAGLAGVGASLGVVIAIVAHTAFLDFTGIIAGIAAAALGLLVLPARKRKAKSEFSEKL
                     TDLRERLVTNLEQQFDREMRRSTQRIEDTIAPFDRFVRSERDRLTKRQTSLQSLSGEI
                     SDLVRQLEPGP"
     gene            2735..3205
                     /locus_tag="PLC06_08120"
     CDS             2735..3205
                     /locus_tag="PLC06_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HRO90448.1"
                     /translation="MAPHPEAITVFKLDHNGREVWQYPATVVERGPDYVRLEALFNRD
                     DMDLGYAVFKRGDRFVEYFYTDRWYNTFAVYDRDDKALKGWYCNICRPAEIDESAVRC
                     DDLALDVWITPAGVSTVLDEDEFITLEIPRDDRRQAGEALQQLLSEANSGRLPY"
     gene            complement(3283..3723)
                     /locus_tag="PLC06_08125"
     CDS             complement(3283..3723)
                     /locus_tag="PLC06_08125"
                     /inference="COORDINATES: protein motif:HMM:NF036688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HRO90449.1"
                     /translation="MDNKNLFISALIGAAVMVVLSNVPVLSLINCLLCAGIWLGGMAA
                     VWFYRRQTGVPVTPGQGAMIGVVAGLIGAVVGSVISSVFGADAMQSVIDADPSGQMQS
                     MLGGFVGGTTTFIISFIFNIVLYPLFGAIGGAIYAVLTQPKKTV"
     gene            complement(3866..4447)
                     /locus_tag="PLC06_08130"
     CDS             complement(3866..4447)
                     /locus_tag="PLC06_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRO90450.1"
                     /translation="MIEAISGLSAAFGLAGSAGLNAYIPLLLVALAARFPMGDPLIKL
                     QAPYDLMGSWWAIGLIAVLLVIEIVADKIPAVDTVNDGIQTFIRPAAGAVLFAGSANV
                     ITDIHPFLALGAGLLVAGGVHATKTAARPVVTVSTAGVGNPIVSTLEDITAFVVSLLA
                     ILLPIIAAILLIMLLIVAALMVRRWRRRKVVTL"
     assembly_gap    4821..4920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5067..6134
                     /gene="ruvB"
                     /locus_tag="PLC06_08135"
     CDS             5067..6134
                     /gene="ruvB"
                     /locus_tag="PLC06_08135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRO90451.1"
                     /translation="MTTEHDPTNRPIAPQQKADDIGLDTLLRPQRLAQFTGQERLKAN
                     LHILIEAARGRGEPLDHVLFHGPPGLGKTTLAQVVANEMGANIRHTAGPAIEHSGDLA
                     AILSNMRSGDILFIDEVHRLSRPVEEILYPAMEDFVLDLIVGKGPGAKNVRLKLPRFT
                     VVGATTRLALMTAPLRARFGAVYRMDFYDDDAMKEIVGRGARILEVPIEPEGMSEIAR
                     RARGTPRVALRLLRRVRDYAQVRADGAITHDTAIAALNLMEIDPLGLDDLDRRVLRAI
                     IEKFAGGPVGLETIAASISEESDTIMDVVEPYLLQLGFLERTSRGRVATANAYEHLGL
                     VAPENDLPGAQRTLFGRATGG"
     gene            6138..6365
                     /locus_tag="PLC06_08140"
     CDS             6138..6365
                     /locus_tag="PLC06_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HRO90452.1"
                     /translation="MVEFGRLLLIIGLAIAFCGLVILVATRYLPWLGNLPGDFRIEGE
                     RYKIYLPLATMILISIFGTVLLNIVLRIFRR"
     gene            6373..6951
                     /locus_tag="PLC06_08145"
     CDS             6373..6951
                     /locus_tag="PLC06_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRO90453.1"
                     /translation="MSRLILFNKPFRVLCQFTDREGRSTLADRPTLADRPTLADYIPV
                     KDVYAAGRLDYDSEGLLLLTDDGRLQQTITDPRHKQPKVYWAQVEGIPDRRAIDRLRG
                     GVRLGDGLTRPARARIIPEPDVWPRVPPIRYRQNIPTAWLELTLTEGRNRQVRRMTAA
                     VGHPTLRLIRWAIGPWTLDGLPPGQWRELSHD"
     gene            6944..7693
                     /locus_tag="PLC06_08150"
     CDS             6944..7693
                     /locus_tag="PLC06_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRO90454.1"
                     /translation="MTSPVAIDILGVPVHPVTMAGTLALVEQYMAEPRLHQIATVNPE
                     FVMAAQDDPDFHRVLHTADLCIPDGVGLLIAARRRGQSLPERVPGSELVGHLAELAAA
                     HGWSLFLLGAAPGVGAAAGVAEQAAQRLVERYPGLVVAGTFAGSPAIEENDDIVRRIN
                     ESGAALLFVAYGAPKQDKWIDRNRDALPAVRVAIGVGGSLDFITGRAIRAPRWMQRLG
                     LEWLHRLIREPWRWRRMLALPRFAWRVIHNS"
     gene            7707..>8617
                     /locus_tag="PLC06_08155"
     CDS             7707..>8617
                     /locus_tag="PLC06_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO90455.1"
                     /translation="MRILITGSSGQIGTNLALHLLEHGHDVFGVDVRPNTWTDAFPTL
                     LQDLAAPFSDFSGGLGHVPYPPNLDVVVHFAAHAKVHELVEQPARALENIVMTFNVLE
                     YCRHNRLPIIFSSSREVYGDIRRYITEESYADFAFTESPYSASKISGEALIYSYAQCY
                     GLRYLVFRFSNVYGRYDVDIERMERVIPLFIRKIARGEPITVFGEKKVLDFTYVDDCV
                     VGVYRGIELLAGAGEANQTINLAYGQGNSLVSLAEYIGEELGVAPNMTVEPSRVGEVT
                     HYVANIGKARALLGYTPQVPLREGIHRA"
BASE COUNT         1722 a         2905 c         2386 g         1504 t
ORIGIN      
        1 gtcatcctca gccagacgct ccagttcctg aacgacccga tgatgatcct gggcgagatg
       61 ttgcgcgtcg gccgcctggc cgtggtcagc ttcccgaact tcggtttctg gcgctgccgg
      121 gcgcacctgt tagtctacgg aaaggtaccc caggcgcccg attacccgca ggcttggcat
      181 gacactccac ggcaacaagc gttcacgata gccgacttca ctgccttctg ccgcgaaaac
      241 agcgtcgagg tacgacagga gacctatctc tccggcgagc ggcatgtcac gcaatggtcc
      301 aacctgctgg cccgtacggc cgttttcgct ctggaacgtc agatataaga cccggcaggc
      361 ttcaaggcaa tctcacaccc gtcggcctgc cgagtctttg ccaatgtttt cgctattccc
      421 agtcgtcagg gagcagcgcc gtggccacac cactgatgat ggtcaggaca accgccccaa
      481 tcacgatatt ccagaagaaa tcaggcatgc aggtgaaacc gatgccgatc gcccgcgaga
      541 gccagatcgt tagccacagc atcaacccgt tgataatgaa caggaagagg ccgagggtca
      601 ggacgaccag aggacaggtc aaaatcgtca ccagcggccg aatgagcgcg ttgacgacgc
      661 cgaagatcag ggcgatgatc agcaacggca gaatgctgcc gtcccagcac aaatcgacga
      721 tccagaacgt cagacccagc gccacagcat tgatcagaat tcgcaatatt aatctcacga
      781 gatacctcct tcgttaccca tccgccttgt cggcggatac tgcaatattt ttactgtcat
      841 tctatacgca attgccggct gccggttcgc acggagcagc aaattacgta taattaccca
      901 agatcgagcc aacaacgcgc tcgacctgat tattatggat gcaatcctga cccaggcaca
      961 ggaaactctg cttaaaagga cacgcgacgc gctcggccga ttgcgcgacg tcctcaatgc
     1021 cgacgccatc aatcccgccg gtcacgccgc cctcgtcgaa tccatccgcc aactggacga
     1081 gcttttcctg ctggtgatcg ccggcgagtt caactccggc aagtcggctt tcatcaacgc
     1141 cctgcttggg caaaagctgc aaccggaagg ggtcacgccg acaaccgatc agatctacct
     1201 gctccgctat ggggagacgc agcagcgcgt acccggcgaa aacggggtct ggattcagac
     1261 cgcgccggtc gagctgctcg gcaagctgac cgtcgtcgac acgccgggaa ccaacgccat
     1321 cgtccgcgag cacgaagcgc tgacctcgga gttcatcccc cgcagcgacc tggtgctgtt
     1381 cgtcacctcg gccgaccgcc ctttcaccga aagcgagcgc gccttcctgg agcaaatccg
     1441 caactggggc aagaagatcg tcctggtgat caacaaaatc gacattctcg gcagcgaggc
     1501 cgacgtcgat caggtgattg ccttcgtcac aaactcggcc cgctcgctca tcggcgacgt
     1561 cgccgcggtc ttcccggtgt cggcgcggct ggcccaggcc gccaagaacg gccaacccga
     1621 caagtggacg cccagtcgtt ttgagccact tgagacctac atccgcgaga cgctcgacga
     1681 tgcgggccgc tttcggctta aactgctcaa cccacttggc gtcggcgcgc gactcacccg
     1741 cgagcagttg gccgacctga acgccgatct gtccacgctg accgaagaca gcaagctgct
     1801 tgatgacatc cgcggccaga ccgtctatta cgacgaggac atgcagcgca acttcaaggc
     1861 gcgcatcgcg gagatcgacg gcttgctgct gggcatggaa aagcgcggtc ttaccttctt
     1921 cgacgaccgg ctgcgattgg gccgaattcc cgaccttctg cgctccaaac agctggagca
     1981 ggattttcag caagaggtcg tggccgacac gccgcgccag atcgaggatc gggtcaacga
     2041 actggtcgac tggatggtgc agcaggattt gcgccaatgg acggctgtgg ccgatcatct
     2101 gggccgcaag cgcgacgtcc accagggccg catcgtcggc gacgcgccgc gcgagggcac
     2161 gctggcctat gatcgccagc gcctcatcga ctccatcggc cgcgccaccc gcgaagcggt
     2221 caacagctat gacgagacgc gagaggcggc cgaactggct gcgtcggcgc ggcaggccgt
     2281 ggtcaatgcc gggctggccg gcgtgggcgc cagtcttggc gtcgtcatcg ccatcgtcgc
     2341 ccacaccgcc ttcctggact tcacgggtat cattgccggg atagctgccg ccgcgctggg
     2401 tctgctcgtc ctgcccgccc gcaagcgcaa ggccaaatcg gagttcagcg agaagctgac
     2461 cgacctgcgg gagcgcctgg tcaccaatct ggagcaacag ttcgaccgcg aaatgcgccg
     2521 cagcacgcag cgcatcgagg ataccatcgc tcccttcgac cgcttcgtcc gctccgagcg
     2581 cgaccgcctg accaagcggc agacgtccct gcaatcgcta tcgggcgaga tcagcgatct
     2641 ggttcgccaa ctggaacccg gcccgtagcc cttcaccggc gacacgaccg caatcgtccg
     2701 gaacgaccgg cgcgcggcat ctcacctgaa cgtaatggcc ccccaccccg aagccatcac
     2761 cgtctttaag ctggaccaca acggccgcga ggtctggcaa tacccggcca ccgtcgtgga
     2821 gcgcggcccg gactatgtgc gcctggaagc acttttcaac cgcgacgata tggacctcgg
     2881 ttatgccgtc ttcaagcgcg gcgatcgctt cgtggaatat ttctacaccg accgttggta
     2941 caacaccttc gccgtctacg accgcgacga caaggcgctg aagggttggt actgcaatat
     3001 ctgtcgtccg gcggagatcg atgagtcggc cgttcgctgc gacgatctgg ctctcgacgt
     3061 ctggataaca ccggcggggg tatctaccgt gcttgatgaa gacgaattta tcacactgga
     3121 gatcccgcgc gacgatcgcc ggcaggcggg cgaagcatta cagcagttac tatccgaagc
     3181 caatagcggt cggctaccct attgacaccc ttgcggttga ctcgtcgatg gccgaccgct
     3241 tctactgccg gtcgcattgc cgatcaagat gatcggttat ctctataccg tctttttcgg
     3301 ctgcgtgagt acagcgtaga tcgcgccgcc aatcgcgccg aacaacgggt agagaactat
     3361 attgaagatg aagctgatga tgaacgtcgt ggtgccgcca acaaagccgc ccaacatgct
     3421 ttgcatctga ccacttgggt cagcgtcgat gacgctctgc atggcatcgg ccccgaaaac
     3481 agaactgatg accgaaccaa ccacggcacc aatcagaccg gccacgacgc cgatcatagc
     3541 tccctggccg ggggtaacag gcacgcccgt ctgccgacga tagaaccaca cagccgccat
     3601 gccacccaac catatgcccg cgcatagcag gcagttgatt aggcttaaaa caggaacatt
     3661 cgataacaca accataacgg ccgcgccgat cagggcagag ataaacaggt ttttattgtc
     3721 catgattcaa agcctccagg attccaaacg cagattataa ggcaggtccc tgcaaacaag
     3781 acaaccttta tcgctcaatc cagttaacca aagcagcttg ccactcacat ttcaacttgg
     3841 acatcacctc cttaacaaac gtttgtcaaa gagtgacgac ctttcgccgc cgccaacgac
     3901 gtaccatcaa tgcggcaacg atgagaagca tgatcaggag gatggccgca ataatcggca
     3961 gcaagatggc cagaagggag acaacgaaag cggtaatgtc ctccaacgtg ctgacgattg
     4021 gattgccaac gccggccgtg gatacggtga cgacagggcg cgcggccgtc ttcgttgcgt
     4081 ggacgccccc cgccaccagc aaccccgcgc ccagcgccag gaacgggtga atatctgtga
     4141 tgacattggc gctgcccgcg aacaatacag cgccggcggc cgggcgaatg aaagtctgga
     4201 tgccgtcatt gactgtgtcg acggcgggga tcttgtcggc cacgatctcg atgaccagca
     4261 ggacggcgat caagccgatg gcccaccaac tgcccatgag atcatagggc gcttgcaact
     4321 tgattagcgg atcacccatg gggaaccggg cagccagcgc caccaataac aagggaatat
     4381 aggcgttcag tccggcgctg cccgcaaggc caaaggcggc gctcagacca ctaattgctt
     4441 caatcatgat aataatagct ccttccaatc acaacccgca caaacaatct attaccctgc
     4501 gaccgggtta tgccggtcgc ttccatttta ctttacgtga aatggatgtc ggggttgccg
     4561 cgaccgggga aggaaagcgc acagtcgcga cagacctgag cattatatca taattgattg
     4621 aatctgctgt gcctgcactg gtgtgggcgc tgtagtgacc cgaaaaagtg gttcttccgc
     4681 aactttcgtg ctcacgctga acgaatgaag agtgcgcacc tccttcgtac ttcgtacttt
     4741 ggacgcgctc ttgcggcgtt ggcgcgacag gagtcgcgtt ggcgcgacag gagtcgcgtt
     4801 ggcgcgacag gagtcgcgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 gacaggagtc gcgttggcgc gacaggagtc gcgttggcgc gacaggagtc gcgttggcgc
     4981 gacaggagtc gcgttcgtct tcattcctaa tccataattc ctaattcata attcctgatt
     5041 ctcatggtat atttgggcaa cccgccatga ccaccgaaca cgaccccacc aaccgcccca
     5101 ttgccccaca gcaaaaagcc gatgacattg gcctcgatac cctgctgcgg ccgcaacgcc
     5161 tcgcccagtt caccggccag gaacggctca aagccaatct ccacatcctc atcgaagccg
     5221 cccgcggccg cggcgagcca ctcgaccacg tgctgttcca tggcccgccc ggcctcggca
     5281 aaaccaccct ggcccaggtc gtcgccaacg agatgggcgc caacatccgc cacaccgccg
     5341 gcccagccat cgaacactcc ggcgacctgg ccgccatcct ctccaacatg cgcagcggcg
     5401 acatcctgtt catcgacgaa gttcaccgcc tcagccgccc cgtcgaggaa atcctctacc
     5461 cggccatgga agatttcgtc ctcgacctca tcgtcggcaa agggccgggg gccaagaacg
     5521 tgcgcctcaa actgccgcgc ttcaccgtcg tcggcgccac cacccggctg gccctgatga
     5581 ccgcccccct gcgcgcccgc ttcggcgcgg tctatcgcat ggacttctac gacgacgatg
     5641 ccatgaaaga gatcgtcggc cgcggcgcgc gcatcctcga agtgcccatc gaaccggagg
     5701 gcatgagcga gatcgcccgc cgcgcccgcg gcaccccgcg cgtcgccctg cggctgctgc
     5761 gccgcgtgcg cgactatgcc caggtgcgcg ctgatggcgc catcactcac gacaccgcca
     5821 tcgccgccct caacctgatg gagatcgacc ccctcggcct cgacgacctc gaccggcgcg
     5881 tgctgcgggc catcatcgag aaattcgccg gcggccccgt cggactggag accatcgccg
     5941 cgtccatcag cgaagaatcc gacaccatca tggacgtcgt ggaaccctat ctgctccagc
     6001 tcggctttct ggaacgcacc tcacgcggcc gcgtggccac cgccaacgcc tatgaacacc
     6061 tggggctggt ggcccccgaa aacgacctgc ccggcgccca gcgcactctg tttggccgcg
     6121 ccacaggagg ttaaacgatg gttgaattcg gccggcttct gctcatcatc ggactggcaa
     6181 tcgccttctg cggcctcgtc atcctcgtcg ccacccgcta cctgccctgg ttgggcaacc
     6241 tgcccggcga cttccgcatc gaaggcgaac gctacaaaat ctacttaccg ctggcgacga
     6301 tgatcctcat cagtatcttc ggcaccgtcc tgctgaatat cgtcctgcgc atctttcgcc
     6361 gctgagtatc ccgtgagccg cctcatcctg ttcaacaagc cgtttcgcgt cctgtgtcaa
     6421 ttcaccgacc gggaagggcg gtcgacattg gccgaccggc cgactttggc cgaccggccg
     6481 actttggccg actacatccc cgtcaaggat gtctacgccg ccgggcgact cgactatgac
     6541 agcgaaggcc tgctgctgtt gaccgacgac ggccgcttgc aacaaaccat caccgacccc
     6601 cgccacaaac aaccgaaagt ctactgggcg caagtggaag gcatccccga ccgccgcgcc
     6661 atcgaccggc tgcgcggcgg cgtccgcctc ggcgacggcc tcacccgccc cgcccgtgcc
     6721 cggatcatac ccgaacccga cgtgtggccg cgcgtcccgc ccattcgcta tcgccaaaac
     6781 atccccaccg cctggctgga gctgacgctg accgaaggcc gcaaccggca ggtgcggcgc
     6841 atgaccgccg ccgtcggcca tcccaccctg cgcctcatcc gctgggccat cggcccctgg
     6901 acgctcgacg gcctgccgcc cggccagtgg cgggaactga gccatgacta gtcctgtagc
     6961 catcgacatc ctcggcgtgc ccgtccatcc cgtgacgatg gccggaacgc tggccctcgt
     7021 cgagcaatac atggccgagc cgcgactgca ccagatcgcc acagtgaacc cggagttcgt
     7081 catggccgcc caggacgacc ccgacttcca tcgcgtcctg cacacggccg atctgtgcat
     7141 ccccgacggc gtggggctgc tcatcgccgc ccgccgccgg gggcagtcgc tgccggagcg
     7201 cgtacccggc tcggagttgg tcggtcacct ggccgaactg gccgccgccc atggctggtc
     7261 actgttcctg ctgggcgcgg cccccggcgt gggcgccgcg gccggcgtag ccgagcaagc
     7321 ggcgcagcgg ttggtcgagc gctatccggg gctggtcgtc gccggaacat ttgccgggtc
     7381 gcccgccatc gaggagaacg acgatatcgt ccgccgcatc aacgagagcg gcgcggccct
     7441 gctgttcgtg gcctatggcg cgccgaagca agataaatgg atcgaccgca accgcgacgc
     7501 cctgcccgcc gtgcgcgtgg ccatcggcgt gggcggctcg ctcgacttca ttaccggccg
     7561 tgccatccgc gccccgcgct ggatgcaacg gttagggctg gaatggctcc accggttgat
     7621 ccgcgaaccc tggcgctggc ggcggatgct ggccctacct cgctttgcgt ggcgagtaat
     7681 tcataattca taattcctaa tctcctatgc gtattctcat caccggctca agcggccaaa
     7741 tcggcactaa tctggcgctc catttgttgg agcacggcca cgacgtcttc ggcgtcgatg
     7801 tgcggcccaa cacctggacc gacgccttcc ccaccctgct gcaagacctg gccgcccctt
     7861 tctccgactt cagcggcggc ctcggccatg tcccctaccc gcccaacctc gacgtcgtcg
     7921 tccacttcgc cgcccacgcc aaagtccacg aactggtgga acaaccggcg cgcgcgctgg
     7981 aaaacatcgt catgaccttc aatgtgctgg aatattgccg ccacaaccgg ctgccgatca
     8041 tcttcagcag ctcgcgcgag gtctacggcg acatccgccg ctatatcacc gaagaatcct
     8101 acgccgactt cgccttcacc gaaagccctt actcggccag caaaatctcc ggcgaggcgc
     8161 tcatctactc ttacgcccaa tgctacggcc tgcgctatct cgttttccgc ttcagcaatg
     8221 tctacggccg ctatgatgtc gacatcgagc gcatggagcg cgtcatcccc ctcttcatcc
     8281 gcaagatcgc ccgcggcgag ccaatcaccg ttttcggcga gaaaaaagtc ctcgatttca
     8341 cctacgtcga cgactgcgtg gtcggcgtct atcggggcat cgagctgctg gccggcgccg
     8401 gcgaggccaa ccagaccatc aacctcgcct atgggcaggg caacagcctc gtgtcgttgg
     8461 ccgaatatat cggcgaagag cttggcgttg cccccaatat gactgttgag ccgtcacgcg
     8521 tcggcgaagt cacccactac gtcgccaaca tcggcaaggc gcgcgccctc ctcggctaca
     8581 ccccccaggt gccgctgcgt gaaggcatcc atcgcgc
//
LOCUS       DAOMQG010000118         8608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3526_length_8608_cov_11.320589, whole genome shotgun sequence.
ACCESSION   DAOMQG010000118 DAOMQG010000000
VERSION     DAOMQG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_8608_cov_11.320589"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1455
                     /gene="miaB"
                     /locus_tag="PLC06_08160"
     CDS             46..1455
                     /gene="miaB"
                     /locus_tag="PLC06_08160"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRO90456.1"
                     /translation="MTKKYHFWITGCQMNYADARRVATELEKLGYQSTPRAEDADVVI
                     LETCTVRQQSEDKTYHKLNSLKPLKSHRPDMTVAVMGCVVGVRGNAVLEKRFPFVDVF
                     MEPATDGLPLINHLTQDSDYDLEQASTLERHALQDFALQDEAVILPVEQRGQIVSAPV
                     AIVYGCSHACTFCIIPQKRGIERSRPIGDIAAEVRSLVAQGVKEVVLLGQIVDRYGYD
                     VADGPDLADLLRAINDIDGLERIRFLTSHPSYMTDRIIRAVAELPKVMPQIEVPIQAG
                     NNEVLEAMKRGYTREEYRTLIHRVRAIIPDAAIHCDVIVGFPGETAEQFQETYDILAE
                     LRLDKIHLARYSPRPGTVSTRRMADDVPDEEKRRRFQLLEALQKDILLEKMRLHLGET
                     VEVLVEERDKGRWRGRTPHNKLVFFDDPRDLRGQLVDVEITHTGPWSMSGRLKDPHRP
                     AAASETIAPAGEIIPLSIL"
     gene            complement(1488..>2435)
                     /locus_tag="PLC06_08165"
     CDS             complement(1488..>2435)
                     /locus_tag="PLC06_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRO90457.1"
                     /translation="VTTICQQRGRYQLGPIRLRTSDPFGLFPMERDLAPTSNVVVFPM
                     TFDIHQLALPVGILPGGDALRRRTHYITTNASSVRDYEPGDSFNRIHWRSTARRDRLV
                     VKEFELDPLADIWVVPDMSVFGHVAPRGKNAAPTPPPSDVPSWLKMKEFQLPESTEEY
                     TVTIAASVAQFFLRRDRAVGMLAYGQSNEILQPDRGERQFNRILETLAVLRAEGEVPL
                     QDMLYAESHLFPRGTTVIVVTPNGREEWAINARQLNRRGLRVVTILVNPASFGSARSA
                     ESLYHLLRGGGQVVYLVNNGDDLTATLSRGATQPSHFAI"
     assembly_gap    2436..2535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2651..3616)
                     /locus_tag="PLC06_08170"
     CDS             complement(2651..3616)
                     /locus_tag="PLC06_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRO90458.1"
                     /translation="MADIQATANKIVESVEQVMVGKRQAVQLTVLGLLCQGHILIEDV
                     PGVGKTVLAKSIAKSVGCKFQRIQFTPDMLPSDVTGVSVFNQQTREFEFRPGPIHAQI
                     VLVDEINRATPKTQSALLEAMEERQVTVDGRTYPLDPPFMVLATQNPIEYEGTFPLPE
                     AQLDRFMLRIRLGYPGKEEEIEILTRQQHRHPVNSLEQVVDVDELRAAQEQIKDIYVD
                     QLVKEYIVNIVRQTREHPDVYLGSSSRGALAIYRLGQARAALLGRDYVLPDDVKALAG
                     PALGHRIIVGPAARIKDIEPEQIVQDIIDQTAVPGAQVGRQKVRA"
     gene            3923..4918
                     /locus_tag="PLC06_08175"
     CDS             3923..4918
                     /locus_tag="PLC06_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90459.1"
                     /translation="MQQKQLNQMALPGVFSTLAAGFDLTTRYLWLVLVPAALDLFLWI
                     GPRLSFRPLIESLAAQLPAQGLPLDMGAMLDLYAARLNHFSYLSVSLMGVPALMSGLT
                     PEKTPIQPLVIERAGWGEWGGYLALFTLGGLLLAALYYSLIAYALRRTAATAAGIAMP
                     PPRLTRRTGRTWFRLLGLGLLFLAFVVAVTFPAALISGFVALINQTMATFVLMGAMVF
                     IVWLIMFFSYTPQGMALNPAGFVQTIVESVRLFRANLPASLGLLFVVTLARRLLDILL
                     LSVDTGTWVTAINLLAHAYICTALIVAMFIFYRDRYTAFLQEARSPAATNQVEQQ"
     gene            4926..6866
                     /gene="gyrB"
                     /locus_tag="PLC06_08180"
     CDS             4926..6866
                     /gene="gyrB"
                     /locus_tag="PLC06_08180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRO90460.1"
                     /translation="MTKTKQNDDISAVHYDAGNIQVLKGLEAVRRRPGMYVGGTDIKA
                     LHHLVFEIVDNSIDEALAGRCGRIEVAIHPDGSISVEDDGFGIPVDEHPTEKVSALQV
                     VHTSLHSGGKFDNAAYKVSGGLHGVGAAAVNALSEWMEVEVKRDGAVWKQRYERGIPM
                     TPVERLRPLKKGEATGTRSTFKFDPTIFTEDNVYRFEMLVTRFREMAFVTSGVFLKMT
                     DERLSPAREMNFYFEGGVRSFVRYLNRNRKVLHDPVYAHKEMENVDVEVSVQYTDGVA
                     ISEFAFANTINTPDGGTHLTGARTALTRVINNYARKNNFLKEKDKNFSSDDTREGVTL
                     VVSVKHPDPQFESQTKVKLMNAEVAGQVSAVLGEALQTFLDENPREAKRIIDRCLTSS
                     RARDAAKAARELVMRKSALDSLTLPGKLADCSERDPTRAELYIVEGESAGGTAKQGRD
                     RHFQAILPLRGKILNTERARLDKILGNNEVKALISALGTGIGESFDLSNLRYGRTIIM
                     TDADVDGSHIRTLLLTFFFRYMPTLIENGHLYIAQPPLYAVKHGKEIAYAYTEAERDA
                     LMKKLNGQKFSIQRYKGLGEMNAEQLWETTMDPGVRTLLQVTVEDAVVADRTFDMLMG
                     DEVAPRRRFITTHASEVRNLDV"
     gene            complement(6982..8583)
                     /locus_tag="PLC06_08185"
     CDS             complement(6982..8583)
                     /locus_tag="PLC06_08185"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRO90461.1"
                     /translation="MLGFIQILGGLALFLYGISLLSSGMEKLAGDQIQKWLDRVTNNR
                     MKSMAFGAASTAVLQSSGLLMVTMIGLINANLMTVTQSISVMLGQEIGTTLTAQIVAF
                     EIGNFRLLLVILGYTFLEFFPKRDWRKFGEILMGLGIIFVGMTYMAGALDELIQIPWV
                     ANLLDLMGQYPLIGVLLGIVATAFTQSSTAVTSMAVAMGMTQVITLEGAIGVILGANI
                     GSCVTGLLASFRLSSSARQASYAQIIINVVGVLIFLPFIPQFAEFIQRTSSELPRQIA
                     NAHTIFNLSVSLALFPFVKQLAAVVKRIAPDDPVVVKEKLTKYIDEMQYAVPSVALNE
                     AARELMHLGDVTQEMVALSCNALLKKEPADAQKVLDMETRMVDLVAKEIEDFVNNLFR
                     SELSMSQYNRALEIKNMTIDVERVGDMAENIANCALNRIAGDITFTTEITQELERLSD
                     FARSIYAQSLAAFRTDDTELALQVRKSENEFDNMYWEARERHDQRLKAGMADPEAEAI
                     FREALRLLERISDHADNLGASVLRT"
BASE COUNT         1845 a         2591 c         2383 g         1689 t
ORIGIN      
        1 cgggccacaa acgaaaacct gcgcctcttc gggccatcaa accgcatgac aaaaaagtac
       61 catttctgga tcaccggttg ccagatgaat tacgccgacg cccggcgtgt cgccaccgaa
      121 ctggagaagc tcggctatca atccacgccg cgcgccgaag atgccgacgt cgtcattctg
      181 gaaacctgca ccgtccgcca gcagtctgag gacaagacct accacaagct gaacagcctg
      241 aagccgctga aaagccatcg gccggacatg accgtggcgg tgatgggctg cgttgtcggt
      301 gtgcgcggaa acgccgtcct ggagaaacgt ttcccgtttg tcgatgtctt catggagccg
      361 gcaaccgacg gcctgccgct catcaaccac ctgacccagg acagcgatta cgatctcgaa
      421 caggcatcga cccttgagcg ccatgccttg caggacttcg ccctgcaaga tgaagcggtc
      481 attctgcccg tcgagcagcg cggccagatc gtgtccgcgc ccgtagccat cgtctacggc
      541 tgctcccatg cctgcacctt ctgcatcatt ccccagaagc gcggcatcga gcgcagccgg
      601 cccataggcg acattgccgc cgaagttcgc tcactcgtgg ctcagggcgt caaggaagtc
      661 gtcttgctgg ggcagatcgt cgatcgctat ggctatgatg tggccgacgg cccggatttg
      721 gccgacctgc tgcgcgccat caacgacatc gacggcctgg agcgcatccg ttttctaacc
      781 agccacccca gctacatgac cgaccgcatt atccgcgccg tggccgaact gccgaaagtg
      841 atgccgcaga tcgaagtgcc cattcaggcc ggcaataacg aagtgctgga ggcgatgaag
      901 cgcggctata ctcgtgagga atatcggacc ctcatccacc gcgtgcgagc catcattccc
      961 gatgccgcca tccattgcga cgtcatcgtc ggcttccccg gcgagacggc cgaacagttc
     1021 caggaaacct acgacatcct ggccgagttg cgcctggaca aaatccatct ggctcgctac
     1081 agtccgcggc cggggaccgt cagcactcgt cgcatggccg acgatgtgcc cgacgaagaa
     1141 aagcgccgcc gtttccaact gctcgaagcg cttcagaaag acattctgct ggagaaaatg
     1201 cgcctgcatc tgggcgagac ggtggaagtt ctggttgaag aacgggataa aggacgctgg
     1261 cgcggccgca cgccccataa caaactagtc ttcttcgacg acccgcgcga tttgcgcggc
     1321 caactggtgg atgtcgagat cacccacacc gggccgtgga gtatgtccgg ccgccttaag
     1381 gacccgcatc gaccggcagc tgcgagcgaa accatcgcac ccgccggcga gataattccc
     1441 ctgtcgatcc tctagcgaga aagctcagcc gaattggctg agctttttta tatcgcgaaa
     1501 tgactgggct gcgtcgcgcc gcggctcagg gtagcggtca gatcatcccc attgttgacc
     1561 aggtagacga cctgcccccc accccgcaaa aggtgataga gcgactcagc cgaacgcgcg
     1621 ctgccaaagg acgccgggtt caccagaatc gtcaccacgc gcaggccacg ccgattcaac
     1681 tggcgcgcat tgatcgccca ttcttcacgc ccgttgggtg tcaccacaat gaccgtcgtg
     1741 ccgcgcggga agagatgcga ctcggcgtag agcatgtcct gcaaggggac ttcgccctcg
     1801 gctcgcagca cggccagcgt ttccaggata cgattgaact gccgctcgcc cctgtccggc
     1861 tgcaggatct cattcgattg cccataggcc agcataccca cagcccggtc acgacgcaga
     1921 aagaactgcg ccacggaagc ggcgatggtg acggtatatt cctcggtcga ttcgggtagc
     1981 tggaactcct tcatctttag ccaacttgga acatcgctcg gcggaggggt tggagccgcg
     2041 tttttgccac gcggagccac atggccaaaa accgacatat cgggcacaac ccaaatatcg
     2101 gccagcggat ccagctcaaa ctctttcacc accaaccggt cgcggcgcgc cgtggagcgc
     2161 caatgaatgc gattgaagct gtcgcccggc tcgtaatcgc gcacgctgga ggcattggtc
     2221 gtgatgtaat gcgtgcggcg acgcaaggca tcgccaccgg gcaagatgcc aaccggcaaa
     2281 gccagctggt ggatgtcgaa ggtcatggga aagacgacga cattgctggt cggtgccaga
     2341 tcgcgctcca tcggaaagag gccgaacggg tcgctcgtgc gcaggcgaat cgggccaagt
     2401 tgatagcggc cgcgctgctg gcagatcgtc gtcacnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnncccaa tagataggaa agcgtaaaaa gcaggtctcg gccggtgaca
     2581 agacccgcaa aaaaggctac aagagccaac atgaggacaa cgatccgtcg ttgcccgaaa
     2641 aacgtcgcca tcaggcgcgc accttctggc ggccgacctg agcgccggga acggcggttt
     2701 gatcgataat gtcctgcacg atctgttccg gctcgatatc cttgatgcgt gccgcgggac
     2761 caacgataat ccgatgaccc agcgcgggac cggccagagc cttgacgtcg tccggcaaca
     2821 cataatcccg gccaaggagc gccgcgcgcg cctgccccaa ccgatagatg gccaacgcgc
     2881 cccgggagct tgaacccaga tatacatccg ggtgttcgcg cgtctgccgc acgatattga
     2941 cgatgtactc cttcaccaac tggtcgacgt agatatcctt gatctgctcc tgcgccgcgc
     3001 gcagttcatc aacatcaacc acctgctcaa gcgagttgac cggatgccga tgctgttggc
     3061 gcgtgagaat ctcgatctct tcttccttgc ccggatagcc cagacggatg cgcagcatga
     3121 agcgatcgag ctgggcttcc ggcagcggga aggtgccctc gtactcgatt ggattctgcg
     3181 tggccaggac catgaatggc ggatccaatg ggtaagtgcg gccgtcgacg gtcacctgtc
     3241 gctcttccat ggcttccagc agcgccgact gggttttcgg cgtggcgcga ttgatctcgt
     3301 cgaccaggac aatttgagca tgaatagggc cggggcgaaa ctcaaattcg cgcgtctgct
     3361 ggttaaatac cgacacgccg gtaacgtcgc tgggcaacat atcgggcgtg aactggatgc
     3421 gctggaattt acagccgacg gacttggcaa ttgatttggc cagcaccgtc ttgcccacgc
     3481 cgggcacgtc ttcaatcagg atgtgaccct ggcacaacaa gcccagcacc gtcagctgca
     3541 cggcctgacg cttgcccacc atgacctgtt ctacgctctc aacaatcttg ttcgcggttg
     3601 cctggatatc tgccatgttc atacctctga aggatagggg aatcgaccat caccccggcc
     3661 agtaagcgca gggacgattc gagacaggcc gacaccgtgg aatgtgggga cggagtataa
     3721 cacgcaattc atcaatggta atattttagt ggcaatttgg ggtcatttcc cgtgtgtcga
     3781 acccctgcgt gaactagttt agcatagcgc gaaccatcag tactcaaaat gaagctaact
     3841 atcggttaat catatggccc ttgattacgc caccaaaact tagccctctc agaacagatg
     3901 tgttataatc catctgttaa ccatgcagca gaaacaactc aaccaaatgg cgcttccagg
     3961 cgtcttttca acgttagccg ccggttttga cctgaccaca cgctacttgt ggctggtgct
     4021 cgttccggcg gcgctcgacc tcttcctttg gattgggccg cgcctcagct ttcggccgct
     4081 gatcgagtca ctggcggccc agttaccggc tcagggcctg ccgttggata tgggcgccat
     4141 gctcgatctg tatgccgccc ggctgaacca tttctcatat ctgagcgtat cgttgatggg
     4201 cgttccggcg ctaatgagcg gcctgacccc ggagaagacc cctatccagc cactggtcat
     4261 tgagcgcgcc ggctggggcg aatggggtgg ctatctggct ttgttcacgc tgggtgggct
     4321 tttgctggcc gccctctatt acagcctcat cgcctatgcc ttgcgccgaa cggccgcgac
     4381 ggccgcggga atcgccatgc ctcccccgcg cctgactcgc cggacaggcc ggacatggtt
     4441 tcgcttgctt gggctggggt tgctgtttct ggccttcgtg gttgccgtca ctttcccggc
     4501 agcgcttatt tccggatttg tcgccctgat caaccagacc atggccactt tcgtcctcat
     4561 gggcgcgatg gtgttcatcg tctggctcat catgtttttc agctacaccc cgcagggtat
     4621 ggctttaaac cctgcgggat tcgtgcaaac gatcgtcgag agcgtgcgct tgtttcgggc
     4681 caaccttccg gcatcgctgg gattgctatt cgttgtcacc ctggcccggc ggctgctgga
     4741 tatcctcctg ctgtcggtcg ataccggcac atgggtgacc gccatcaatt tattggccca
     4801 tgcctacatc tgcaccgcac tcatcgtggc catgtttatt ttttatcgcg atcgctatac
     4861 ggcgtttctg caagaagcgc gatcgccggc cgccacaaac caggtagagc aacaataacc
     4921 ctatgatgac caagaccaaa caaaacgacg acatctcggc agtccattac gacgccggaa
     4981 atatccaggt actcaagggg ctggaagcag ttcgtcgccg gcccggtatg tatgtcggcg
     5041 gcactgatat caaggccctg caccatctcg tcttcgaaat tgtcgacaac tccatcgacg
     5101 aggcgctggc tggccgctgc ggccgaattg aagtggcgat tcatcccgat ggcagcatta
     5161 gtgtggaaga cgacggcttc ggtatcccgg ttgatgagca cccgaccgag aaggtgtcgg
     5221 ccctgcaggt cgtccacacc agcctccatt ccggcggcaa gttcgacaac gcagcctaca
     5281 aagtatcggg cggccttcat ggcgtcggcg cggcggccgt gaatgcgttg tcggagtgga
     5341 tggaagtcga ggtcaaacgc gacggagccg tgtggaagca gcgctatgag cgcggcatcc
     5401 cgatgacgcc tgtggagcgg ctgcgccccc tgaagaaagg tgaagcgacc ggcacgcggt
     5461 caacctttaa gtttgaccca actattttca ccgaggacaa cgtctaccgc tttgagatgc
     5521 tcgtcacgcg gttccgcgag atggcttttg tcaccagcgg cgtcttcctg aagatgaccg
     5581 acgagcgcct gtcgcccgcc cgcgagatga acttctactt cgagggtggc gtccgctctt
     5641 ttgtgcgcta tctcaaccgc aaccgcaagg tgcttcacga tcccgtctat gcccacaaag
     5701 agatggaaaa cgtcgatgtc gaagtctcgg tgcaatacac cgacggcgtc gccatctccg
     5761 aattcgcttt cgccaacacg atcaacacgc ctgacggcgg cacgcatctg accggcgcac
     5821 gcaccgcgtt gacgcgtgtc atcaacaact acgcccgtaa aaacaacttc ctgaaggaga
     5881 aggacaagaa cttctcttcc gacgacacgc gcgagggcgt caccctggtc gtcagcgtga
     5941 agcaccccga cccgcagttc gaaagccaga ccaaggttaa actgatgaac gccgaggtcg
     6001 ccgggcaggt gtcggccgtg ttgggcgagg cgttacagac atttcttgac gaaaacccgc
     6061 gcgaggccaa gcgcatcatc gaccgctgcc tgacctcctc acgcgcgcgc gacgccgcca
     6121 aggccgcccg cgaactggtc atgcgcaaga gcgcgcttga tagcctgacc ctgccgggca
     6181 agctggccga ctgctccgaa cgcgacccca cccgcgccga actctacatc gtcgagggtg
     6241 aatcagccgg tggcacggcc aagcagggac gcgaccgcca tttccaggcc atcctcccgc
     6301 tgcgcggcaa aatcctgaac acggagcgcg cccggctgga caagattttg ggcaacaatg
     6361 aagtcaaggc gctcatctcg gcgctgggca cgggcatcgg cgagtcgttt gacctgagca
     6421 acctgcgtta cggccgcacg atcatcatga ccgacgccga tgtggacggc agtcatatcc
     6481 gcacgctgct gctgacgttc ttcttccgct atatgcccac gctgatcgag aacggccatc
     6541 tctatatcgc ccaaccgccg ctttatgccg tgaaacatgg caaggagatc gcctatgcct
     6601 acaccgaggc cgaacgtgac gcgctgatga agaagctcaa cgggcagaag ttcagcattc
     6661 agcgctacaa gggtttgggc gagatgaacg cggagcagtt atgggagacg acgatggatc
     6721 ccggcgtccg caccttgctg caggtgaccg tggaagacgc cgtggtggct gaccggacat
     6781 tcgacatgct aatgggcgac gaagttgcgc cccgtcgccg cttcatcact acccacgcca
     6841 gcgaggtgag gaacctggac gtgtaggtta ctgcgcctca cgcaaggtag ttatagcaag
     6901 caccgcggcc gaaaccgcgg tgctttttca tgtcatgcag atgcccgata tgctcgaagc
     6961 attccctcgc cgacgtatga ctcaggttcg caatacgctg gcgcccaggt tatccgcatg
     7021 atcactaata cgctccagca ggcgcaaagc ctccctgaaa attgcttccg cctcaggatc
     7081 agccatcccc gcttttaatc gttgatcgtg cctctcgcga gcttcccaat acatgttatc
     7141 aaactcgttc tctgacttgc gcacctgaag cgccaactct gtatcatccg tccggaaggc
     7201 tgcgagagat tgggcataga tcgatcgcgc gaaatccgag agccgttcca gctcctgagt
     7261 aatttcggtg gtaaaggtga tatccccggc aatcctgttc agggcgcagt tggcgatatt
     7321 ctccgccata tcacccacgc gctcgacatc aatcgtcata tttttaattt ccaaagctcg
     7381 attgtactgg ctcatgctca gttcggagcg gaaaaggtta ttgacgaaat cctcaatttc
     7441 cttcgcgacc agatcgacca tccgggtttc catatccagg actttctgcg catcggccgg
     7501 ctcctttttc agcaaagcgt tgcaactgag ggcgaccatc tcctgggtta cgtccccaag
     7561 gtgcatcagt tcgcgcgccg cttcattgag cgcaaccgac gggacggcgt attgcatctc
     7621 atcaatatac ttcgtgagct tttccttcac gacaaccgga tcatcagggg ctatccgctt
     7681 aactaccgca gcaagttgtt tgacgaacgg gaatagcgcc aggctgaccg acaaattgaa
     7741 gatggtgtgg gcgttagcga tctgtcgggg gagttcggat gacgttcgtt gaatgaactc
     7801 cgcaaactgg ggaatgaaag gcaggaagat cagcacaccg actacattga tgatgatttg
     7861 agcataagat gcctgccggg cagaagacga caaacggaat gatgcgagaa ggcctgtcac
     7921 acaggagcca atattggcgc caaggatcac cccgatcgcg ccctccagcg tgatgacctg
     7981 ggtcataccc atagcaacgg ccatgctggt aacggccgtg ctgctttggg tgaaggccgt
     8041 tgcaacgatg cccaacaaca cgccgatcaa ggggtactga cccataagat cgaggagatt
     8101 ggcgacccac gggatctgaa tcagctcgtc cagcgcgccg gccatgtacg tcatcccgac
     8161 gaagataatg ccgaggccca tcagaatctc gccaaacttt cgccagtcgc gtttggggaa
     8221 aaattcaaga aaggtataac cgaggatgac caaaagcaac cggaagttgc cgatctcaaa
     8281 tgcgacgatc tgggcagtta gagtggtgcc aatctcctgc cccagcatga cactaatgga
     8341 ctgcgtcacg gtcatcagat tggcattgat caagccaatc atcgtcacca tcaacaaacc
     8401 actgctttgc aacacggcgg tggaagccgc gccgaaagcc atactcttca tgcggttgtt
     8461 cgttacgcga tcaagccatt tctggatttg atccccggcc agtttctcca tgccggagct
     8521 aagcaaggag atgccataga gaaacagggc tagtccgccc aatatctgaa taaagcctag
     8581 catactccct ccggaactat ggggctat
//
LOCUS       DAOMQG010000119         8574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3547_length_8574_cov_10.066205, whole genome shotgun sequence.
ACCESSION   DAOMQG010000119 DAOMQG010000000
VERSION     DAOMQG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_8574_cov_10.066205"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLC06_08190"
     CDS             complement(<1..524)
                     /locus_tag="PLC06_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO90462.1"
                     /translation="MKTHVFDAVIIGAGGAGLMTALYASRGANVAVISKLYPTRSHTG
                     AAQGGIGAALGNLEEDHWEWHAYDTVKGSDYLADQDAVDVLCKEAIDTVIELEHMGLP
                     FDRLPNGRISQRRFGGHTNNNTGDPVMRACHAADRTGHMILQTLYQQCIKNKVNFFDE
                     YHVVDLIRTADSIRG"
     gene            complement(557..967)
                     /locus_tag="PLC06_08195"
     CDS             complement(557..967)
                     /locus_tag="PLC06_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90463.1"
                     /translation="MAAISDKSPGKITVRRVQIERSFDRGGWLFMRLSGVAMLILAVG
                     HMFIQHILNSSTTLTLDFVAVQWSSWGWKAYDILLLWMAIPHGIRGLYTVLTDYIHNP
                     GLKRPIGILLALFVVATVIWATIGITLFNPAAVR"
     gene            complement(979..1443)
                     /gene="sdhC"
                     /locus_tag="PLC06_08200"
     CDS             complement(979..1443)
                     /gene="sdhC"
                     /locus_tag="PLC06_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRO90464.1"
                     /translation="MSSLILTVRESIRYRGRSGQYSWIAHRISGLAILFFLVIHVWDT
                     ANAHFWPQAYAWSLALFKTPFFGLGEIGIMAAVLYHAFNGIRITILDFKPEWWRHQRT
                     SSIITWALFLLAFIPAGILMFSGMIGHCTALASVGDSCFRIPAFSEFAQYAR"
     gene            complement(1841..>2232)
                     /locus_tag="PLC06_08205"
     CDS             complement(1841..>2232)
                     /locus_tag="PLC06_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO90465.1"
                     /translation="PSYWGTPLAIMADIDPDRVLFVFGARPSKESLIPGDVVPDALHQ
                     ALKALADPTRLRILRYLTDEPLAPVELARRLRLRSPTVIHHLDALRLARLVIVTIEPE
                     GKRYAVRPDAVEDVFSLLNNFLVGRDV"
     assembly_gap    2233..2332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2333..3066)
                     /locus_tag="PLC06_08210"
     CDS             complement(<2333..3066)
                     /locus_tag="PLC06_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90466.1"
                     /translation="MELSIKNRIEWDIGTAYDFFVSLWALHEPGRLGLRGSWAAGVRS
                     RLSGPDREILQRAMPLVRPMRWTYSLPAPKNAQTALDELRAMDPEARLLALMPETPPQ
                     ITSIWRDLAARSSWNEEDQMRLMEEMAGGDWKKHSVTAVRKAAAEYLDLWMDPIGAAD
                     SLLAAYQCYYDVFFAEEEPRIRPALEEGLAHAQMMAEQIQQWDKLLEELSQGLRVTKD
                     WENKTLVLAPSYWGTPLAIMADIDPDR"
     gene            complement(3079..3696)
                     /locus_tag="PLC06_08215"
     CDS             complement(3079..3696)
                     /locus_tag="PLC06_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO90467.1"
                     /translation="MAMELRTRQVGPWLMNTYALVCPKTRQSVLFDPGDDPDTLSEML
                     AGTLPVAILLTHTHPDHIGALSEMRRRLGVPLGAFDGPHLNGLTLAEEILLRDDNLFT
                     VGKHTLRVYSTPGHIPDQICFLVDGDNKAIVGDTLFDGGPGKTWSAAGFRQTLDSLHI
                     VLGWPNDIVCYPGHGPHFRLGDRRAQIETFVERDHGDFFGDATWD"
     gene            complement(3784..4548)
                     /locus_tag="PLC06_08220"
     CDS             complement(3784..4548)
                     /locus_tag="PLC06_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90468.1"
                     /translation="MSEQDPTRRFTDRVENYARYRPGYPSALLECLREECGLTPGSVI
                     ADIGSGTGILTEMFLDNGNPVFAVEPNEAMRQSAEISLARYPGFTSVNGRAEATTLPS
                     SSIDLVTAGQAFHWFDATASRIEFGRILRPGGYVALVWNARDHEEDPLMAAYEHVLGE
                     FGMGYTTVTHRSHDGDLDTLFINGRKFRSFTHTRRMNFATLWGGFLSASYAPSPNDAI
                     YEPMRAGLRSVFETYQHDGQVTFIYKTHLYFGQLTN"
     gene            complement(4545..4907)
                     /locus_tag="PLC06_08225"
     CDS             complement(4545..4907)
                     /locus_tag="PLC06_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO90469.1"
                     /translation="MITGIHHVQITISPTDEAAARTFYIDLLDLPEVEKPDSLKGRGG
                     FWIEVGDRQVHIGVEDGVDRRRTKAHVAYQVDDLNAWRERLAAAGIAALNSVPIPGFD
                     RFECRDPFGNRLEFIQPL"
     gene            5022..6317
                     /locus_tag="PLC06_08230"
     CDS             5022..6317
                     /locus_tag="PLC06_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90470.1"
                     /translation="MEPLPVDIFQLQRRDPLAWSALLARCPETSDAVVTAVTSEPLCD
                     QSPAMPAHLLLSDCSHQVRRYTLTLVGVSDPISFIVKRTSAVEARFYSLYGDPPGTAI
                     PRCHYTHLDGEESWVVLDDIPADYSSTDWVPDQVDDIVGTLARIHAARWNYDLEVDGG
                     LGQVEPLIPHFLHRRSGPYRWDELREMETALFEEGPSVALSRHAIHSAGRLAPLFLRA
                     ANGLVVMRALDGWPGVMGESHLSAVADLLDDPVPMLAPLLDLPLTLLHGAPHPGHWRL
                     TLFDDAYLVDWSEVQIGPGVLDLMAFIEGYPLLQFGPRERRGQSSQSGLLGKLSLLAE
                     ETIVDTYLLTLSAELGSRSAARAYRAALPAARCLHVLLTWFPYFATWAGNMPDRYVWQ
                     RVNRRTETEVGRYHDTPDAGLRQYLAGVFERFLCACRSL"
     gene            6363..6866
                     /locus_tag="PLC06_08235"
     CDS             6363..6866
                     /locus_tag="PLC06_08235"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRO90471.1"
                     /translation="MDNIFYSINTGIVILAFVVGLLGAILPVIPGLTVIWLGALYYAA
                     IIVGFDTFSPWVFVLLTIVGLVAGTSNIWLAALGAKTTGASWRTLIVGFIGAILGTFF
                     LPIPLVGTIVGYAAGLVLSEYVRLGELRPALKSAFGGVVGWGVSTAFEFAGGLAMILL
                     VVTQAPR"
     gene            6883..7272
                     /locus_tag="PLC06_08240"
     CDS             6883..7272
                     /locus_tag="PLC06_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90472.1"
                     /translation="MEEFLQNFVLILLGMMSVALVLAIILLIIIFIQIRHIKVPPNAG
                     FAETLLYTPLLVVLFLDVLDLALDFLSAPIAWVVLDRLGLKGLRGVATVESFIPFTQV
                     IPAMTLAWIGVRLFGKDRFIEQKYYEQ"
     gene            complement(7333..>8574)
                     /locus_tag="PLC06_08245"
     CDS             complement(7333..>8574)
                     /locus_tag="PLC06_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO90473.1"
                     /translation="LAIHPSPVAALFALPLRPAGEFQGALYLAYRQPHYFDSDERNLL
                     RTLAGQATVLVQNAHLFVAAEGGRRRLAAILASTTNGILVTDQTDRILLINPAMERAL
                     GVRGKDAAGRPVVDALSGIDKAIPFVQRLSLSRSGTGSGATDGKVELEVNDRTFLASI
                     STVYSHEGQTMGRVAVLQDVTDMKELDRMKSDFVAGISHDLLSPLTYMHNYAAMLPIV
                     DDPTLEKEYVEKIMAGIDRMTRLVNDLLELARIEAGLHLQFDRVHVDKLLHEIAMEYA
                     SPAKAAGVNLVVEVADDLPAAVADPAQLRRALTNLVTNGLKHAANSGPLTIHAEAIGR
                     EMVISVRDRGPGIAAVDQTHLFEKFYRGEGLSTVERAKGSGLGLAIVKSVADHHNGRV
                     WCESRSDEGSTFYLAIPLKRE"
BASE COUNT         1716 a         2449 c         2422 g         1887 t
ORIGIN      
        1 ccgcggatgg agtcagcagt gcggataagg tcgacgacgt ggtattcgtc gaagaagttg
       61 actttgttct tgatgcattg ctggtaaagc gtctgcagga tcatatgacc ggtgcggtcg
      121 gcggcgtggc aggcgcgcat tacgggatcg ccggtgttgt tgttggtgtg gccgccgaaa
      181 cgtcgctggg aaatacggcc gttcggcagg cggtcaaacg gcaatcccat atgctccagt
      241 tcgatgacgg tgtcgatagc ttccttgcag agcacatcga ccgcatcctg atccgccaga
      301 tagtcggaac ccttgaccgt gtcgtaggcg tgccattccc aatgatcttc ttccagattg
      361 ccgagggctg cgccaatgcc gccctgagct gcgccggtat gggagcgagt ggggtagagt
      421 ttggagataa cggcgacgtt cgcccctcgg ctggcgtaga gagcagtcat cagccccgca
      481 cccccagcgc caatgatgac tgcgtcgaat acatgcgttt tcataagtac cttccagata
      541 cctcgtggtt aaatgcctaa cgaaccgcgg ccgggttgaa taatgttatg ccgatcgtgg
      601 cccagatgac ggtggcgacg acgaataggg ccaagagaat accaatcggc cgcttgaggc
      661 cagggttgtg aatataatcg gtcagaaccg tatagagtcc acgaatgcca tgagggatgg
      721 ccatccagag caacaggatg tcgtaggcct tccagcccca tgaactccat tgtacggcta
      781 cgaagtcgag cgtcagggtt gttgagctgt tcaatatgtg ttggataaac atatggccaa
      841 cggccaggat gagcatggcg acgcccgaaa ggcgcatgaa aagccagccg ccacgatcaa
      901 agctgcgctc aatctgaact cggcgaacag tgatttttcc aggtgattta tcggatattg
      961 cagccatttc atgtactcct agcgggcgta ttgagcgaac tcactgaaag ccgggatgcg
     1021 gaagcagcta tcgcccacac ttgccagcgc ggtgcaatga ccaatcatgc cgctaaacat
     1081 caggatcccg gccgggatga atgcgagcag aaataaggcc caggtgatga tggagcttgt
     1141 tcgctggtgt cgccaccatt ccggcttgaa gtcgagaata gtaatgcgaa taccgttgaa
     1201 cgcatgatac aggacggcgg ccatgatccc gatttcgccg aggccgaaga acggggtctt
     1261 gaacagcgcc agtgaccagg cataagcctg tggccaaaag tgggcattag ccgtatccca
     1321 gacatgaatg acaaggaaga agaggatggc caacccggag atacgatgcg ctatccagct
     1381 gtattgccct gagcgcccac gatagcgaat actttcacgt acggtgagga ttaaagaact
     1441 cataaagggg atctcctgtc tgaaaatcat ggttagtata gcatgtagat aaaaggcgag
     1501 ctattttaga gcgtgatgat tgcggcctgt aaggcgccct ccccgtgggt ctatcggcgc
     1561 attaaagtgc ttggtaggcg atacatctgt tgctcccgga tatatccctc gcgtttttgc
     1621 ttaaagctcc cctttcctct tccgcttttt gtttgtggtt gacttgaaaa tccaactggt
     1681 tattcacggt ctatttactg agtttagatc agtactatag tactatgtat gcacggtgac
     1741 ttacgccagt aaatcactat gcgggtggta ttcagggaga aggagcaaat ggagacatgg
     1801 gcttgcttcg cgagcctgtg tctcccccct gatcaatctt ctaaacatcc cggccgacca
     1861 aaaaattatt tagcaggcta aagacatcct ccactgcgtc gggccgcaca gcgtagcgct
     1921 tcccctcggg ttcaatggtc acgatgacca atcgtgccag ccgcagggca tccaggtgat
     1981 ggatgacggt tggcgaacgc agccgcagcc gccgcgccag ctcgaccgga gccagcggct
     2041 catccgttag atagcgcagg atacgcaagc gagttgggtc ggccagcgcc ttgagcgcct
     2101 ggtggagcgc gtcgggcacg acatcgccgg gaatcagcga ctccttcgac ggccgcgcgc
     2161 caaaaacgaa caatacccga tccggatcga tgtcggccat tattgccaac ggcgtacccc
     2221 aataagatgg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgatccgg
     2341 atcgatgtcg gccattattg ccaacggcgt accccaataa gatggggcca gaacgagcgt
     2401 cttgttttcc caatccttgg tcacgcgcaa gccttgggac aattcttcca gcagcttgtc
     2461 ccattgctgg atttgttccg ccatcatttg ggcgtgcgcc agcccttcct ccaatgccgg
     2521 gcgaatgcga ggttcttcct cggcaaaaaa gacatcgtaa tagcactgat aggctgcaag
     2581 caagctgtcg gccgctccaa tcggatccat ccacaagtca agatattccg cggcggcctt
     2641 gcgaacagca gttaccgagt gctttttcca gtcccccccg gccatctcct ccatgagacg
     2701 catctgatct tcttcgttcc aggagctacg agcagccaga tcgcgccata ttgaggtaat
     2761 ttggggtggt gtttcaggca tcagcgctaa taatcgcgct tcggggtcca tggcgcgcaa
     2821 ttcatccaat gcagtttgag cgttcttggg cgcgggaagt gaataggtcc accgcatcgg
     2881 ccgcaccagt ggcatcgccc gctgcaatat ttcgcgatca ggcccggaca ggcgcgaacg
     2941 aaccccggcc gcccatgatc ctcgcaggcc aagcctcccc ggttcatgga gcgcccataa
     3001 actaacgaag aaatcgtatg cggttccgat atcccattca atgcggtttt taattgaaag
     3061 ttccataatg acacactact agtcccaggt ggcgtcgccg aagaaatccc cgtgatcgcg
     3121 ctctacgaac gtctcgattt gggcacgtcg gtcgcccaac cgaaaatgag ggccgtggcc
     3181 tggatagcaa acgatgtcat tgggccagcc cagcacgatg tgcagggagt ccagggtctg
     3241 acggaagccg gcggccgacc atgtcttgcc gggtccgccg tcgaagagtg tatcgcctac
     3301 gatggccttg ttgtcgccat cgaccagaaa acagatctga tcaggaatat ggccgggtgt
     3361 ggaataaacc cgtaacgtgt gtttgcccac ggtaaacaga ttgtcgtcgc gcagtaagat
     3421 ctcctcggcg agcgtaagtc cgttaaggtg cggcccatcg aatgcgccca gcggcacgcc
     3481 gagtcgccgt cgcatttcac tcaatgcccc aatatgatcg gggtgggtgt gggtcagcag
     3541 gatggccacc ggcagggttc cggccagcat ctcgctcagc gtgtcggggt cgtcaccggg
     3601 atcgaagaga acgctttgcc gagtcttagg gcagactagc gcgtaggtgt tcattaacca
     3661 tgggccgact tgtcgggtgc gcaattccat tgccattatg tgctcctcgc ctaaataccc
     3721 cgttgcattc agtggtcgct caatcccctg tcgttcgcct gatattgtgt cggataggtg
     3781 tcatcagttc gtcaactgcc caaaatacag atgcgtcttg tagatgaaag tcacctggcc
     3841 atcatgttga tacgtctcga agacagatcg caaccccgcg cgcatgggtt cgtaaatcgc
     3901 gtcattgggg gatggcgcgt aggaggctga cagaaacccg ccccaaagcg tggcgaagtt
     3961 catccggcgt gtgtgggtaa agctgcggaa cttccgtcca ttgatgaaca gggtatctaa
     4021 atcaccatca tgggaacgat gggttacagt cgtatagccc atgccgaatt cgcccagcac
     4081 gtgttcatag gcggccatca atggatcttc ttcgtggtcg cgggcgttcc acaccagagc
     4141 tacgtacccg ccgggtcgca ggatgcggcc gaactctatc cggctggcag tagcgtcgaa
     4201 ccaatgaaag gcctgcccgg cagtcacaag gtcgatactg ctgctgggca gagttgtcgc
     4261 ttcggcgcgg ccgttcacgc tagtgaagcc gggatagcgt gccaatgata tctcagccga
     4321 ctgcctcatc gcctcattag gctcgactgc aaagacagga ttgccgttgt ccaggaacat
     4381 ctccgtcaga atgcccgtgc cggagccaat atcggcgatc accgagccag gcgtcagccc
     4441 gcactcttct ctcaggcact ccagcagggc agagggatag ccgggacggt aacgagcgta
     4501 attctcaacc ctgtctgtga aacggcgtgt ggggtcttgc tcgctcataa cggctggata
     4561 aactccaggc gattgccaaa cggatcgcga cactcgaacc gatcgaagcc ggggatgggc
     4621 acgctgttca gtgctgcgat gccggccgcc gccagccgtt cccgccacgc attcaggtca
     4681 tcgacctgat acgcgacatg ggctttcgtc cgccggcgat caacgccatc ctcgacgccg
     4741 atatggactt gacgatcacc gacctcaatc cagaaaccac cgcgtccttt taaggaatcg
     4801 ggcttctcaa cttccggcag gtcgagcaga tcaatataga atgtccgggc ggccgcttcg
     4861 tcggttggcg agattgttat ttggacgtga tgaatgcctg taatcatgat atagggattt
     4921 taaccaataa cacgctctag tcacccaaac gaccgattat tcacagtcgc acccttagcc
     4981 tgcttgcagt gcgccgcaat ccccattaca atttcgcccc tatggaaccg ttgccggtgg
     5041 atattttcca gcttcagcgc cgcgatccgc tggcgtggtc ggccttgctc gcccgctgtc
     5101 ccgagacaag tgatgcagtc gtcacggccg ttacgtcgga gccgctgtgt gatcaatcac
     5161 cggctatgcc cgctcacttg ttgctctctg attgttccca tcaagtccgc cgctataccc
     5221 tgactctggt cggcgtgtcc gaccccatca gtttcatcgt caagcgaacc agcgccgtgg
     5281 aagcccgctt ctatagtctg tatggcgatc cgcccggcac agctatccca aggtgccatt
     5341 acactcacct cgatggcgag gagagttggg tcgtccttga cgacatcccc gccgattatt
     5401 catccactga ttgggtgccg gatcaagttg atgacattgt agggactctg gctcgcatcc
     5461 atgccgcgcg ctggaactat gacctggaag tggatggtgg cctggggcaa gttgagccgc
     5521 tcatccccca tttcctgcac cgccgaagcg gcccctatcg ctgggacgag ctgcgtgaaa
     5581 tggaaacggc gctcttcgaa gaggggccga gcgtcgcgtt gtctcgccac gccattcaca
     5641 gcgccgggcg gttggcgcct ctcttcctgc gtgcggccaa tggccttgtc gtcatgcgcg
     5701 cgctcgacgg ctggcccggc gtgatgggtg aaagccatct gtcggctgtg gctgacctgc
     5761 tcgatgatcc cgtgcccatg ctggctccct tgctcgatct gccgctaacg ctgcttcacg
     5821 gcgctcctca tcccggccac tggcggctaa cattgtttga tgacgcctat ctcgttgact
     5881 ggagtgaggt gcaaatcggg ccaggcgttc tcgatctgat ggcctttatt gagggttacc
     5941 cgctgcttca gttcggtccg cgcgagcgac gggggcaatc ctcacaaagc ggcctcctcg
     6001 gaaagctgtc gctattggct gaggaaacaa tcgtcgatac ctatctgctg accctgtcgg
     6061 ccgagttagg cagccgctcc gcggcgcgcg cctatcgggc agcactgccg gctgcgcgtt
     6121 gcctccatgt cctgctgacc tggttcccct atttcgccac ctgggccggc aatatgcccg
     6181 atcgctatgt gtggcagcga gttaatcgtc gaacggagac cgaagtcgga cgctatcatg
     6241 acacaccgga tgcaggtttg cggcaatatc tggccggtgt gttcgagcgc ttcctgtgcg
     6301 cctgccgcag cctctgagcc ctcacgcaac cggcattgca accctgcgta tagcacatgg
     6361 aaatggataa catattttac tctatcaaca cgggcattgt gattctggcc tttgttgtgg
     6421 gcctcctggg cgcaattctg cccgtcatcc ccggccttac tgtgatctgg ctcggggcgc
     6481 tctactacgc cgccattatc gtcggctttg acactttctc cccgtgggtt ttcgttctcc
     6541 tcacgatcgt tggcctggtt gccggcacat cgaacatctg gctcgctgcg ctgggagcca
     6601 agacaaccgg tgcctcgtgg cgaaccctga tagttggctt cattggcgcg atcctcggca
     6661 cattcttttt acccattcct ctggtgggca cgattgtggg ttacgcggcc gggctggtgc
     6721 tatcggaata cgtgcggctg ggcgagctgc gaccggccct taaatctgcc tttggcggcg
     6781 tggttggctg gggcgtcagc acggcattcg agttcgccgg tggcctggca atgatcctgc
     6841 ttgtcgtcac gcaagcccca cgctgaggtt gctttgaccg gaatggaaga atttctacag
     6901 aattttgttt tgatcctgct gggcatgatg tccgtcgctc tcgttctggc catcatcctg
     6961 ttgatcatca tctttatcca gatccgtcac attaaagtcc cgccgaatgc gggcttcgcc
     7021 gagacgttgc tctatacccc tctccttgtg gtgttgttcc tcgacgtgct ggatttggcg
     7081 cttgatttcc tttctgcgcc gatcgcctgg gttgtcctcg atcgacttgg tctgaagggg
     7141 ctgcgtggcg tcgccacggt ggaatcgttt atcccgttta ctcaggtcat ccccgccatg
     7201 acactggcct ggattggcgt cagattattc ggcaaagatc gattcattga gcaaaagtat
     7261 tacgagcaat aggctggtct gagtaagagg gagattctca caggcagatt gcgatcgatt
     7321 acaataaccg ggttactctc gcttgagagg gatagccaga tagaaggtgc tgccctcatc
     7381 ggagcggctc tcgcaccaca cgcggccgtt gtgatgatcg gccactgact tgacgatggc
     7441 caatcccaat cccgatcctt tggcccgttc gactgtggat agaccttccc cgcgatagaa
     7501 tttttcgaaa agatgcgtct ggtcgaccgc ggcgatccct gggccgcgat cgcgaaccga
     7561 gatcaccatc tcgcgaccga ttgcctcggc gtgaatggtc agcgggccgc tattggcggc
     7621 atgcttcagg ccattggtca ccaggttggt gagcgcccga cgcaattgcg cgggatcggc
     7681 cacggccgcc ggcaagtcgt cagccacctc gaccaccagg ttcacgccgg ccgctttggc
     7741 cggcgacgcg tactccatcg cgatctcgtg cagcaatttg tccacatgca cccgatcgaa
     7801 ctgcaagtga agcccggcct cgatgcgcgc cagctccagc agatcattca ccagccgcgt
     7861 catgcggtcg atgccggcca tgatcttctc gacgtactct ttttccagcg tcggatcatc
     7921 cacgataggc agcatggcgg cgtagttgtg catgtaggtc aatgggctta gcagatcgtg
     7981 ggaaatgccg gcgacgaaat cggacttcat acggtccaat tccttcatgt cggtcacatc
     8041 ctgcagcacg gcgactcgcc ccatcgtttg cccctcatgg ctgtaaacgg tggagatgct
     8101 cgccaggaag gttcggtcat tgacttccaa ttcaaccttg ccgtcggtcg cgccgcttcc
     8161 ggttcccgac cggctcagac tcagccgctg gacgaacggg attgctttgt cgatgcctga
     8221 cagagcatcg accaccggac ggccagccgc atctttgccg cgaacgccca acgcgcgctc
     8281 catggcgggg ttgatcagca atatcctatc agtctggtcg gtcacaagga tgccgttagt
     8341 cgttgaggcg aggatcgctg ccaatcggcg tcggccgcct tcggcagcta caaacaggtg
     8401 ggcattttgc accagcacgg tggcttgccc ggccagcgtc ctcagtagat tgcgctcatc
     8461 gctatcaaaa taatggggct gccggtaagc caggtagagc gcgccctgga attcgccggc
     8521 gggccgcaac ggcagcgcga atagcgccgc taccggggat ggatggatcg ccag
//
LOCUS       DAOMQG010000120         8563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3550_length_8563_cov_11.084509, whole genome shotgun sequence.
ACCESSION   DAOMQG010000120 DAOMQG010000000
VERSION     DAOMQG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3550_length_8563_cov_11.084509"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..294
                     /locus_tag="PLC06_08250"
     CDS             52..294
                     /locus_tag="PLC06_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90474.1"
                     /translation="MRLSVIIQLIEEDPIIGELEGLPDPAAQFVTLLNPRRRDGRPAS
                     FLDSNVDQVLFAWHRISHIQVLPDVDLEKVISFVRD"
     gene            331..1062
                     /locus_tag="PLC06_08255"
     CDS             331..1062
                     /locus_tag="PLC06_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO90475.1"
                     /translation="MVEQIPENLEKTAPQLILIVDDELRMRRFIRMNLELEGYQVIEA
                     ENGIQALDQIRQFNPDLAIMDVMMPEMDGFETLKLLREISTMPVILLTVRNDEEDITR
                     GLSLGADDYITKPFSPRVLTSRVGAVLRRAHWPAPPPRTVLRIDDRLSIDFNRHQVIV
                     NGERIDLRPTEYRLLNHLIQNAGWVVPHDTLLAKVWGYEYRDETHYLRLYINYLRKKI
                     EEDPANPKYILTERGVGYRFVDFRK"
     gene            1281..3548
                     /locus_tag="PLC06_08260"
     CDS             1281..3548
                     /locus_tag="PLC06_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRO90476.1"
                     /translation="MAKVYMVDRDLSVADAEQLITLTGTYLNTADRLQVMKAFEFARR
                     EHGNQRRMTGELFFTHPLTVAIYLAEYRLDAAAISAALLHDIAEDTRISLEQISDEFG
                     PEVARLVDGVTKLKEVTSGVAEADRALTAEEVQQATLRKLLQAMTADVRVVIIKLFDR
                     LHNMRTIRGMPPHRQRAKAEETLSVYAPLAYRLGMWNVKSELENRSLEVLQPHVYESI
                     RAELERQVRAQAPLFEVAREQIIECLTENGLPVIDVRMSPENIYTVYRDLEKQGAGFR
                     DIDRMLRITVLLEDTIHCYTALGYIHQLWPAVPKRLDDYISVPRENLYQSLHTTLVHT
                     DGQPIKVRFRTVDMERIAQIGVLTKWYYAGTPLWSPGLAERVKEFFNSISKSINVEPS
                     DPAAGARGAVEDVLSEQIRVYSKDGDPYELPQGSTPLDFAYKVHTALGNQTHDALVND
                     LPFPLNRPLRNGDQIRINKHSRAQPRRAWLDEDLGYITTRLARSYAQRWFHKLSKSEA
                     TRQGQTILLHELRMLGLGDLDHGAIAPLFGYASAGELYYKLGRADLLPTALSTRIMSD
                     HWDKGLSLSKGDVVCAADGSTYIITHANNNKLHLCGTCSPHPGDIIVGYLRQDGILTV
                     HHEGCRTLNADRDRPEINMRMLKLGWGETDSREARIVPLLVDVYDRPGLLYEMTYLMQ
                     RKDINIRHICTYRPKMDSRLYIEMELEVSSPRQMVRILHQIEALVNVRAVRAVPDGFD
                     KPAASSKSSVYYWPE"
     gene            3803..4096
                     /locus_tag="PLC06_08265"
     CDS             3803..4096
                     /locus_tag="PLC06_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005492702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HRO90477.1"
                     /translation="MLKKKYLKTKDEYEVTFQLPTNAEEVVLVCEANGWEPVVMKKGK
                     DAYTAKLRLPANGSYQYRFLVNNSEWVNDDAADKYVPNEHGTTNSVVETFSAN"
     gene            4240..4923
                     /locus_tag="PLC06_08270"
     CDS             4240..4923
                     /locus_tag="PLC06_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRO90478.1"
                     /translation="MSPRPTTEQTIRRLRAVPLFQTLDDATMARLAAETQWYEYRTGE
                     VIVPEGEAQPGLYFLAYGYVKAVKYSAAGREQTLRVLEAGDTFNELSVFTRRPNPATA
                     IALEASGIWLIPRATLTRLMREQPDFAQSVIEQIAERMLFLVSLVTDLSLRPVTGRLA
                     RLLLESASEDVVLRPRWFTQAELAARLGTVPDVIQRSLRGMEGDGLIAVDRQQIRILD
                     RFALEALAV"
     gene            5055..5366
                     /locus_tag="PLC06_08275"
     CDS             5055..5366
                     /locus_tag="PLC06_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRO90479.1"
                     /translation="MIEIQHIPDWQQKVVYGADGPQPTILTEDDRVRVLIAGLKPGQR
                     IPEHPEGTSIYHFLAGSGWMIVNGERLAVAAGTTIVMPAGTVRGMEAETQLAFLATRI
                     V"
     gene            5418..6641
                     /locus_tag="PLC06_08280"
     CDS             5418..6641
                     /locus_tag="PLC06_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90480.1"
                     /translation="MNLYYLMSALFIGAAVLVAADASLTSLDILPWFNGLRWVRVHLV
                     TLGAMTEAIFGTLSQLLAIRYNRPRPAFRWDIWLSLNAGILMLLIGIPIINMTFIYTG
                     GTLIFIATSLLTAQLWGMRPTTNDKSATNEHTGRPFYITGLGFFLLGIIIGTGLWFGW
                     ADWLFISVPLETHIHANNWGLMSLVFAGLLIDTFPTWARRPLAWPRSIKPIFWLMTFG
                     ALGLVLGPWFSSRWFTVPGLIMHQTATFWLVLNIVKPLWGDRQMLSQPGPWHIITSYA
                     WISAPVLVAPLILLGIRGVPGPTIEANAPQALVYGWVFQFGFALLPFLFRRAFLPHEP
                     ARLGGDWFSLAAANLGGVFLWASIFSEPLGPTLHAIAYILWTAAIVPIAIELWRITRQ
                     GLARLEMISAEAAKE"
     gene            complement(6722..7921)
                     /locus_tag="PLC06_08285"
     CDS             complement(6722..7921)
                     /locus_tag="PLC06_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRO90481.1"
                     /translation="MTAGALGDIRVIDLSRVLAGPYCAMLLADYGADVIKIEQPGDGD
                     PTRAWGPPWFGDQAIRESAYYLTANRNKRGMTLNLKTAAGQAIARRLIATADVVIENF
                     LPGAPAAYGLDYATLAAAQPGLIYCSITGYGQTGPYRDRPGYDFMIQAEGGIMSITGP
                     ADGEPHKVGVAVVDITAGLYAVTAILAALHHRATTGRGQWIDVALFDAQLGWLANVAS
                     NYLVSGEPPARYGNAHPNIVPYETFPTADGHIAVGIGSDEQWRRFCELAGRPELAEEP
                     RFATNAARVAARGELIGLLREVFGRRPSAEWLAALAAARIPSAPINDVPMALNDPQTA
                     AREMVRTVTHPAGPLSLVGPVARLSETPATVRAAPPLLGEHTGAILGELGYSVEEIDE
                     LRRDGVV"
     gene            complement(7934..>8563)
                     /locus_tag="PLC06_08290"
     CDS             complement(7934..>8563)
                     /locus_tag="PLC06_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRO90482.1"
                     /translation="AILDVNYGGSTGYGRTYRERLNGNWGIVDVDDCANGARYLVEQG
                     VVDGGRLAIRGGSAGGYTTLRAITARDVFRAAASHYGVSDAEALATDTHKFESRYLDN
                     LIGPYPERRDIYVERSPIYAADRISAALILFQGLDDKVVPPDQSEAMFIAARNKELPV
                     AYIAYEGEGHGFRQAQNIKRTIEAELYFYGRVFGFEPAGDIEPVTIENL"
BASE COUNT         1702 a         2785 c         2439 g         1637 t
ORIGIN      
        1 ggattactgg tacaactgac gatcgcggat taaacttggg acaggtgcgc catgcgatta
       61 tccgtcatca ttcaactcat cgaagaagac cccatcatcg gagaattgga agggttgccc
      121 gatcccgccg cccaattcgt cacactcctg aacccccgac ggcgagacgg ccgccccgcc
      181 tcctttctgg acagcaacgt cgaccaagtc ctgttcgcct ggcaccgcat cagtcacatt
      241 caggtattgc cggacgtcga tctggaaaag gtcatcagct tcgtccgcga ctaggacagg
      301 acgacccaat gcgcctgtaa ggcccttatc atggtagaac aaatccccga gaatctggaa
      361 aaaaccgcgc cgcagctcat cctgatcgtc gatgacgaac tgcgcatgcg ccgcttcatc
      421 cgcatgaatc tggagctgga aggctatcag gtcatcgagg ccgaaaacgg aatccaggcg
      481 ctggatcaga ttcgccagtt caaccccgat ctggccatca tggacgtgat gatgccggag
      541 atggacggct tcgagaccct gaagctgctg cgcgagatct ccaccatgcc ggtcatcctg
      601 ctgaccgtgc gcaacgacga agaagatatc acccgcggcc tcagcctggg ggccgacgac
      661 tacatcacca agccgttcag cccgcgcgtg ctgaccagcc gcgtgggggc cgtgctgcgc
      721 cgcgcccatt ggcccgcccc gccgccccgc acggtgctgc gcatcgacga ccgcctgtcc
      781 atcgacttca accggcacca ggtcatcgtc aacggcgaac gcatcgacct gcggccgacc
      841 gaatatcgcc tgctcaacca cctcatccag aacgcgggct gggtggtgcc ccacgacacg
      901 ctgctggcca aggtgtgggg ctacgaatac cgcgacgaaa cccactacct gcgcctctac
      961 atcaactacc tgcgcaagaa gatcgaggaa gacccggcca accccaaata catcctcacc
     1021 gagcgcggcg tcggctatcg gttcgtcgat ttcaggaaat agccgcccgt ccctgtggcg
     1081 caagttgcca acttgcctct tcgccccgcc cgtacatgct accatcaagg ttatatgcga
     1141 ttgccctgcc tgcttgatcc ggttcgattc ctgagttcgc cttgatatcc ccgcccgaaa
     1201 cagggtataa tgaggggata tacctatcgg gttgcatctt gccgtcccca ctagcggcac
     1261 ccttcacgca accggcaggt atggcgaaag tttatatggt cgaccgcgat ctatcagttg
     1321 ccgacgccga acagctgatt actcttaccg gtacatacct caatactgcc gatcgccttc
     1381 aggtcatgaa agcgttcgag ttcgctcgcc gagagcacgg caaccaacgc cgtatgaccg
     1441 gcgagctttt ttttacccac cccctgacgg tggctatcta tctggctgaa tatcggctgg
     1501 acgcggcggc tatctcggcc gcgctgctcc acgatatcgc cgaagacacg cgcatctcgc
     1561 tcgaacaaat cagcgacgaa ttcgggccgg aggtggcccg cctggtcgac ggcgtgacca
     1621 agctgaaaga agtgacgagc ggcgtggccg aagcggatcg cgcgctgacg gccgaagagg
     1681 tgcagcaagc cacgctccgc aagctgctgc aagccatgac cgccgacgtg cgcgtcgtca
     1741 tcatcaagct gttcgaccgg ctgcacaaca tgcgcaccat ccgcggcatg cccccccacc
     1801 gccaacgcgc caaagcggaa gaaaccttgt cggtctacgc ccccctggcc tatcgactgg
     1861 ggatgtggaa cgtgaaaagc gagctggaga atcgctcgct ggaggtcttg cagccccacg
     1921 tctacgaatc catccgcgcc gagcttgaac gccaggtgcg cgcccaggca cccctcttcg
     1981 aggtcgcccg cgagcagatc atcgaatgcc tgacggagaa cggcctgccg gtcatcgacg
     2041 tcaggatgtc gcccgaaaac atctacaccg tctaccgcga cctggaaaag cagggggccg
     2101 gcttccgtga tatcgaccgg atgctgcgaa tcactgtgct gctcgaagac accatccatt
     2161 gttacaccgc cctcggctat atccatcagc tatggcccgc cgtacccaaa cgtctcgatg
     2221 attacatctc cgtcccccgc gaaaacctct accaatccct gcacaccaca ctcgtccata
     2281 ccgacggcca acccatcaag gttcgcttcc gcacagtcga tatggagcga atcgcccaaa
     2341 tcggcgtcct gaccaaatgg tactacgccg gcacgccgtt gtggtcgccc ggcctggccg
     2401 agcgcgtcaa ggagttcttc aacagcatct cgaagagcat caacgtcgag ccgtcggatc
     2461 ccgccgccgg ggcacgtggc gccgtcgagg acgtactgag cgagcaaatc cgggtctatt
     2521 cgaaggacgg cgacccctat gaactgcccc agggcagcac accgctggac ttcgcctata
     2581 aagtccacac ggcgctcggt aaccagaccc acgacgcgct ggtcaacgat ctgccgtttc
     2641 cgctcaatcg gccgctgagg aacggcgacc agatacgcat caacaagcat tcccgcgccc
     2701 agccccgccg agcgtggctg gatgaagatt tgggctacat caccacccgg ctggctcgct
     2761 cctacgccca gcgctggttc cacaaacttt cgaaatccga agcgacccgg caaggacaaa
     2821 ccatcctgct ccatgaactg cggatgctgg gactgggcga tctcgaccac ggagccatcg
     2881 ctccgctatt cggctatgcg agcgcggggg agttgtacta caaactcggc cgcgccgatt
     2941 tgctgccgac cgcgctctcc acgcgcatca tgtccgacca ttgggacaag gggctttctc
     3001 tatcgaaggg ggacgtcgtc tgcgccgccg acggctccac ctacatcatc acccacgcca
     3061 acaacaacaa gctccatctg tgcgggacat gctcacccca ccccggagat atcatcgtcg
     3121 gctatctgcg gcaggatggt atcctgaccg tccatcatga agggtgccga acgctcaacg
     3181 ccgaccgcga tcgccccgag ataaacatgc gcatgctcaa gctgggttgg ggcgagacgg
     3241 acagccgcga agcccgcatc gtgccgctcc tcgtcgacgt ctatgatcgg cccggcctgt
     3301 tatacgagat gacctatctg atgcagcgaa aggatatcaa catccgccac atctgtacct
     3361 atcggccgaa gatggacagc cggctctaca tcgagatgga gctggaagtg agcagcccgc
     3421 ggcagatggt gcgcatcctg catcagatcg aggcgctggt gaacgtgcgc gccgtgcgcg
     3481 ccgtgcccga cggcttcgac aagccggccg cgtcgagcaa atcatcagtc tattactggc
     3541 ctgaatagcg ccaagcttcg acggaacggc cgcgccatca tcgctgccat tcccccataa
     3601 cccctgccaa atcgtctccc agtctgataa ttagctgaga gaatggcgtt tattattgat
     3661 ccaccatgcc ccgacgtatt gacaccgtcg gggcgattcc tataatcaag agtggccctg
     3721 cggattatcg acgggccaat cccgaagttt ttccaaattt agcttaagga aacacactac
     3781 ctttcttgaa ggagaattaa ccatgttgaa aaagaagtat ctgaagacga aggatgaata
     3841 cgaagtgacc ttccagctgc ccacgaacgc ggaagaagtt gtgctggtct gcgaggccaa
     3901 cggctgggag ccggtggtga tgaagaaggg caaggacgca tacaccgcca agctgcgcct
     3961 gccggccaac ggcagctacc agtatcgctt cctggtcaac aactccgagt gggtgaacga
     4021 cgacgcggcc gacaagtacg tgcccaacga gcacggcacg accaacagcg tggtcgagac
     4081 cttcagcgcg aactaagcgt attcaattca ttcctgtaga ttcgcgcgac tctccgtgat
     4141 tcgcggcatg gacggtctgc cttgggcggg ccgtttttgt tttcccattc gcccggcggc
     4201 aactgccggt ttcgccgggc aaagtgctat aatcccccca tgtcaccccg cccaacaacc
     4261 gagcaaacca ttcgacgact gcgcgctgtg cctctcttcc aaacgctgga cgacgcgacg
     4321 atggcccgac tggcggccga aacgcaatgg tatgaatatc ggacgggcga ggtcattgtg
     4381 cctgaaggtg aagcccagcc cggcctctat tttttagcct atggctatgt caaggccgtg
     4441 aaatattccg ctgcggggcg cgaacagaca ttgcgcgtcc tggaggccgg cgacaccttc
     4501 aatgagttga gcgtattcac acgccgcccc aatccggcca cagccatcgc gctggaggca
     4561 tccggcatct ggctcatccc tcgcgccacc ctcacccgcc tcatgcgcga gcaacccgat
     4621 ttcgcccaga gcgtcattga gcaaatagcc gagcgaatgc tgtttctcgt ttcgctcgtc
     4681 accgatcttt ctctgcgacc ggtgacgggg cgactggcac ggctgctgct ggagagcgcc
     4741 agcgaggacg tcgtgctgcg gcctcgatgg ttcacacagg ccgaactggc ggcgcgcctc
     4801 ggcaccgtac ccgacgtcat ccagcgctcg ttgcgcggca tggaaggtga cggattgatc
     4861 gccgtcgatc ggcagcaaat tcgcatcctg gatcgctttg ctctggaagc gctcgccgtc
     4921 tgatatttcg ctacccgaca aaagtcggcg actttcgcct gattctgcac tatgctgcga
     4981 cccagattcg tgaggcgcgc tactgcaaca caagttcaat gatataactg attttactta
     5041 aggagagcac actcatgatt gaaatacaac acattcccga ttggcaacaa aaagtggtct
     5101 atggagccga tggcccacaa cccacaatcc tgacggaaga cgacagggtc agagtcctca
     5161 tagccgggct gaagccgggc caacgtattc ccgaacaccc ggaggggacg tcgatctatc
     5221 atttcctggc cggttccggc tggatgatcg tgaatggcga acggctggcc gtggccgccg
     5281 gaacaaccat cgttatgccg gccggcactg tgcgcgggat ggaagcggag acgcaactgg
     5341 cttttctcgc aacgcgcatc gtataactga ctttcatcgc gcctggcggc ggcctcaacc
     5401 ctgcaagagt gatctctatg aatttgtatt atctgatgag tgctctgttt attggtgcgg
     5461 ccgtgttggt cgcggctgac gcttcgctaa ccagcctgga tatattgccc tggttcaatg
     5521 gattgcgctg ggtgcgcgtc catctggtga cgctgggcgc gatgaccgaa gccatcttcg
     5581 gcacgctctc ccagctcctg gccattcgct ataatcggcc gcgaccggct ttccgctggg
     5641 acatctggct ctcgctcaat gccggcatcc tgatgcttct gatcggtatc ccgatcatca
     5701 acatgacatt catctacacc ggcgggacgc ttatcttcat tgctacgagc ttgttgaccg
     5761 cccagctctg gggtatgcgt ccgacaacca acgacaaatc cgcgacgaac gaacacaccg
     5821 gccggccatt ctacatcacc ggtctgggct tcttcttgct gggcatcatc atcggcaccg
     5881 gcctgtggtt cggctgggcc gattggctat tcattagtgt cccattggag acccacatcc
     5941 acgccaataa ttggggcctg atgtccctgg tgttcgccgg gctgctcatc gacaccttcc
     6001 caacatgggc caggcggccg ctcgcctggc cgcgctcgat caagccaatc ttctggttga
     6061 tgacattcgg cgcgctgggg ctggtgttgg ggccgtggtt tagctcacgg tggttcaccg
     6121 taccggggtt gatcatgcat cagacggcca cgttctggct ggtactcaac attgtcaagc
     6181 cactatgggg cgaccgccaa atgctttccc aaccaggacc atggcacatc atcacttcat
     6241 acgcgtggat ttcggcccct gtgttggtag cgccgctcat tttgctgggt attcgcggcg
     6301 ttcccggccc gaccatcgaa gccaacgcgc cgcaagccct cgtctatggt tgggtgttcc
     6361 agttcggctt cgcactgttg cccttcctgt tccgacgagc cttcctgccc cacgaaccgg
     6421 cacggctggg gggtgactgg ttcagtctgg cggcggctaa cttgggtggg gtgttcctgt
     6481 gggccagcat ctttagcgag ccgctggggc caacgctcca cgccattgcc tatatcctgt
     6541 ggaccgcggc gattgtgccg atcgccatcg aactgtggcg gattacccga caagggttgg
     6601 cgcggctgga gatgatcagc gcggaagcgg cgaaggaatg agtgagtaag ggtgggacaa
     6661 gacggccgaa tggcatttgt ttgtgattta cgtggctgcc gtcctgtttc gtcttgtttg
     6721 tctaaacgac cccgtcacgc cgcagctcat caatctcctc aaccgaataa cccaactccc
     6781 ccaatatcgc ccccgtgtgc tcgcccagca gcggtggggc cgcgcgcacc gtggccggcg
     6841 tttcgctcag cctggccacc ggcccaacca gcgacaatgg accggccgga tgcgtcaccg
     6901 tccgcaccat ctcgcgcgcg gccgtctgcg ggtcattgag cgccatgggc acgtcgttga
     6961 tcggcgcgga agggatgcgc gccgccgcca acgccgccag ccactcggcc gacggccgcc
     7021 taccaaacac ctcgcgcaac aggccgatga gttccccgcg cgcggccact cgcgccgcgt
     7081 tggtggcgaa gcgcggttcc tcggccagct ccggccgccc ggccaactca cagaagcgtc
     7141 gccattgctc gtcactgccg atgcccacgg cgatatgacc gtcggccgtc gggaacgtct
     7201 cgtagggcac aatattgggg tgggcgttgc cgtagcgagc gggcggttcg ccgctgacca
     7261 gatagttgga cgccacattg gccagccagc caagctgggc gtcgaacaag gccacgtcga
     7321 tccattggcc gcggccggtc gtcgcccggt gatgcagcgc ggccaggata gctgtgaccg
     7381 catacagccc ggccgtgatg tcgaccaccg ccacacccac cttgtgcggt tctccatccg
     7441 ccgggccggt tattgacata atgccgccct cggcctggat catgaagtca taccccggcc
     7501 ggtcgcgata aggtccggtc tggccgtaac cggtgataga gcagtagatc aggccgggct
     7561 gcgccgccgc gagggtggcg tagtccaatc cataggcggc cggcgcgccg ggcaggaagt
     7621 tctcgatcac cacgtcggcc gtggcgatga gacggcgggc aatcgcctgc cccgcggccg
     7681 tcttgaggtt gagcgtcatc ccccgcttgt tgcggttggc ggtcaaatag taggcgctct
     7741 cccgaatggc ctggtcaccg aaccagggcg gcccccacgc ccgcgtcggg tcgccgtcgc
     7801 ccggctgctc gatcttgatc acgtccgcgc catagtcggc cagcagcatg gcgcagtacg
     7861 gcccggccag cacgcgggac aagtcgatga ccctgatatc gcccaaggct ccagccgtca
     7921 tgctgcctcc gcgctacaga ttctcaattg tcaccggctc gatgtcaccg gccggttcaa
     7981 agccaaacac gcgaccatag aagtacagct cggcctctat cgtccgcttg atgttctgcg
     8041 cctgccggaa gccgtgaccc tcgccctcat aggcgatata ggccaccggc agctccttgt
     8101 tgcgcgcggc gatgaacatc gcttccgact gatccggcgg cacgaccttg tcgtccagcc
     8161 cctggaacag gatcaacgcc gccgagatcc ggtcggccgc ataaatgggc gagcgctcga
     8221 cgtagatatc gcgccgttcg ggataggggc caatcaggtt gtccaggtag cgtgactcga
     8281 atttgtgcgt gtcggtggct aatgcttcag cgtcgctgac gccgtagtgg ctggcggcgg
     8341 ctcggaaaac gtcgcgagca gtgatggcgc gcaaagtcgt atatccccct gcactgccgc
     8401 cgcgaatggc cagtcgcccg ccgtctacaa ccccttgctc aacgagatag cgcgcgccgt
     8461 tggcgcaatc gtcgacatcg acgatccccc aattgccgtt gagtcgctcg cgataggtac
     8521 ggccgtagcc ggtgctgccg ccgtagttga catcgaggat ggc
//
LOCUS       DAOMQG010000121         8450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3605_length_8450_cov_10.847290, whole genome shotgun sequence.
ACCESSION   DAOMQG010000121 DAOMQG010000000
VERSION     DAOMQG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_8450_cov_10.847290"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLC06_08295"
     CDS             complement(<1..620)
                     /locus_tag="PLC06_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRO90483.1"
                     /translation="MTNYRTQQALIAMIRRVQSARGRRQAGLFSIEGTRLVERALRAD
                     APLEAVLVADSLIRSGEPRARELKAALQLAGCEIAVAPDDLLRELTEGRDLGLILGLV
                     RLPPALGLADVLRDQGLSQQKPPLLLAAIDIVDPGNVGALTRTAHAGGAVALAAVGVS
                     DPYHSRATRISRGSIFRLPIITYETAAALLADLRHHGIAAAGTVARG"
     gene            755..1639
                     /gene="hslO"
                     /locus_tag="PLC06_08300"
     CDS             755..1639
                     /gene="hslO"
                     /locus_tag="PLC06_08300"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRO90484.1"
                     /translation="MKDYMHLAMAKKEGVRGYAAATTDLVEAARLRHNTSPTATVALG
                     RALTAAVLMSGLLKVGQRVALKWEGTGPLRRVVAEADAKGRVRGYVAEPQVDLPLVQG
                     EYDVPSAIGAAGLLTVVRDLHLPELATGVVHLVGSHIDDDLLFFLEQSDQVPSAVEIG
                     VTLNEDGSVAEAGGILIQPLPPYDPAAIAQLKERLQELPPIEAMLAEGKKPEQILDEV
                     FVGITHTLLSKYPVRFECNCSREQTRALLVSLGREELEGILEHEGQAVVNCQFCHEEY
                     VFDREDLLDILQMMEEEE"
     gene            complement(1716..1789)
                     /locus_tag="PLC06_08305"
     tRNA            complement(1716..1789)
                     /locus_tag="PLC06_08305"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1753..1755),aa:Pro,seq:cgg)
     gene            1943..2992
                     /locus_tag="PLC06_08310"
     CDS             1943..2992
                     /locus_tag="PLC06_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRO90485.1"
                     /translation="MKSRNLRLLIGVIISAVFIWLALRGLHLGDVWASLRAADYWWLI
                     PSVAVYFLAVWARTWRWDYLLRPMKHISLRRLFPVVVIGYMGNNVYPFRAGELLRAYV
                     LRQQEGVSMSSSLATIVVERVFDGLVMLLFVFVALPFAPLPSDSIRFIVMIGSVAFLG
                     ALLVFFAVAAVPARFMALTEWCANRFLPERFRAPIIDFAQRFIVGLASLRSGRGLMMI
                     FLTSVVIWLLETVKYWFVMHAFDFTVSFFALMLMNGVVNLATTLPSAPGYIGTFDGPG
                     IAVLTLYGVDPATAAAYTLTLHAALWLPVTLLGIYYMIRTELGWGDIGRAVDAGREMA
                     EHPNEVAEPHKEVSA"
     gene            2989..4335
                     /locus_tag="PLC06_08315"
     CDS             2989..4335
                     /locus_tag="PLC06_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRO90486.1"
                     /translation="MKFAIIGAGIAGLSAAYDLLRAGHQVTLLEGDNRTGGLATGFQD
                     EQWEWPLEKFYHHLFTSDSEIIGLVEEIGLKDKLFFPRPVTSVIYQDRIVPFDSPKRW
                     ITFPGFNLLDVARFGTVSAYLRFTSPWRELEQHLADDWLRRYYGDKIYEMIWRPMLIG
                     KFGPYYRDVNMAWMWARLHVRSPRLGYFEGGFQAFIDRLTEVVGELGGEVRLNCPAEQ
                     IRPAEDGGVEVTAGGKTEKYDACITTTSPGLLARMAPDLAGDYLGQLLKLDSMGAVVL
                     TLALKESLLAESGTYWLNIPATSPDKAGGVPFLALVEHTNYIDRSHYGGDHIIYCGDY
                     VTPDHPYLTMDQAELEKLFVGVLHRFNPNFRPEWVRKSWLFRARYAQPVPKLNHSQLV
                     PDLRTPMSGLYFASMSQVYPWDRGTNFAVEIGRRVARLALADAADGRLSPRGENGR"
     gene            4549..>4799
                     /locus_tag="PLC06_08320"
     CDS             4549..>4799
                     /locus_tag="PLC06_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRO90487.1"
                     /translation="MKIVVIGTGYVGLPHAAVLSEYGHEVHAYDIDETRIAAYQSGEA
                     GNIERYVNEPGLADIVAENVNRYLFFTTDVTGVVEETDV"
     assembly_gap    4800..4899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4900..5580
                     /locus_tag="PLC06_08325"
     CDS             <4900..5580
                     /locus_tag="PLC06_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HRO90488.1"
                     /translation="WGSYVSNGAGGSCFGKDIQSLIYQLNHAGRPTDLLRAVYNINEY
                     QKIYLIDRAIHEAHFNFNQKKVALLGLSFKKRTNDMRDSAALRVAESLLSRGVATIQA
                     YDPLAMDEARRNWFNPEKNYLYERITYHDSAVEAIEGTDALYISTDWEEFRGLARTIR
                     TTVKPPYLVIDGRRMLPDFENLVAAGYDYLPVGGLLMRGRSNSSEAYSENDQAIPAID
                     ELPQQVPA"
     gene            5708..6193
                     /gene="greA"
                     /locus_tag="PLC06_08330"
     CDS             5708..6193
                     /gene="greA"
                     /locus_tag="PLC06_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRO90489.1"
                     /translation="MLQKKPTLLTQEGLERLTKELEHLRTVGREEVAERLHQAFQDGQ
                     DDDFVDNAELEAARNAQAFLEGRIQELDEILNNYQIITEDMAPHDRVKLGDWVTVTEV
                     GADELERYQLVGPLEADPVAGRISNESPLGRLLVGVKVGEVVRVNAPRGITEFRVVQV
                     E"
     gene            6273..7784
                     /gene="lysS"
                     /locus_tag="PLC06_08335"
     CDS             6273..7784
                     /gene="lysS"
                     /locus_tag="PLC06_08335"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRO90490.1"
                     /translation="MSWEPNPLEEQRLEKLARLQDEGIDPYPLRVERTHTNSEAIATF
                     EAADEGVPVPVTVCGRLISVRDMGKTIFAHIADEDGRLQLFVRREDVGEESHAVFKKL
                     LDLGDFVQASGEMFRTKLGEVSLHVRDWRILSKAISPLPVAKEQEVDGKTVRYSAFAD
                     MEERYRQRYADLAVNHEVRDIFRKRARLFSALRRFMDDNGFLEVETPILQPLYGGAAA
                     RPFTTYHNQLKQELYLRISFELYLKRLLVGGIERVYEIGRDFRNEGVSFKHNPEFTQL
                     EFYAAYWDYRDVMDFTERMVAYAAEAVLGTSDVSYHGHTIHLGAPWMRLTMREAIKQF
                     AEIDYMDYPDAPALAKAIKGIGGDAPPDAPWGKLIDGLLGDFVEPHLIQPTIIMEYPR
                     DISPLAKGVPGDPLHVERFEFFIGGMEMGNAFTELNDPLEQRKRFEALQTTFAKEDEE
                     LNPIDEDYLRAMRYGMPPNGGFGMGLDRLAMLLTDSRTLREVLLFPHLRDRED"
     gene            complement(7806..8357)
                     /gene="msrA"
                     /locus_tag="PLC06_08340"
     CDS             complement(7806..8357)
                     /gene="msrA"
                     /locus_tag="PLC06_08340"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRO90491.1"
                     /translation="MSNNKLEVATLGGGCFWCLEAIYRDVQGVDRVVSGYSGGHVANP
                     TYEQVCGKHTGHAEVVQVWFDPAVISYDEILYIFWRIHDPTTPNRQGNDIGPQYRSVI
                     FYHDEAQKAAAERIRDETAAAKVWPNPIVTEIVPFDVFYEAEGYHQDYYVNNPNQPYC
                     AYVIDPKVRKFRKSFQEKLKQPA"
BASE COUNT         1607 a         2365 c         2582 g         1796 t
ORIGIN      
        1 cctctggcga ctgttcccgc ggcggcgatg ccgtgatggc gcaagtcggc gagcagggcg
       61 gcggccgtct cgtaggtaat tatcggcaat ctgaagatgc tgccgcggct gatgcgggtg
      121 gcgcgtgagt gatatggatc gctgactccc acggctgcca gagcgaccgc accgccggcg
      181 tgggccgtgc gggttaaggc tcccacgttg ccggggtcga cgatatcgat ggcggccaac
      241 agcagagggg gcttttgctg cgacaggccc tgatctcgta gaacgtctgc caggccaagc
      301 gccggcggca ggcgtaccag ccccaaaatt aaccccaggt cgcgaccctc ggtcagctcc
      361 cgcagcagat cgtccggcgc gacggcgatc tcgcagccgg cgagctgcaa tgcagccttc
      421 agctcgcgcg ctctcggctc gccgctgcga atcagactat cggcgacgag aacggcttcc
      481 agcggcgcat cggcccgcaa ggcgcgctcg accaggcgcg tgccctcgat ggagaaaagc
      541 ccggcctggc gacggccgcg agcgctctgc acgcgccgga tcatggcgat gagggcttgt
      601 tgagttcggt agttggtcat gagtagccgg ctgattttac ccgcgtcggt tgcgattgtt
      661 aagcttgagg taagattgtt gctctttgac ccgtctgatt atagaaaccg ggttcctttg
      721 aggcccggtt ttatactcta ttggagaaag tagtgtgaag gattacatgc atttggcgat
      781 ggccaaaaaa gaaggcgtgc gcggctatgc ggcggcgaca accgatcttg ttgaagcggc
      841 ccgcttgcgc cacaacacca gcccgacagc gactgtggcc ctgggtcgtg cgctgacggc
      901 cgccgtgctg atgagtggtt tgctgaaggt cggccagcgc gtcgccctga aatgggaagg
      961 caccggccca ctgcggcgag tcgtagccga ggccgacgcc aaggggcgcg tgcgtggcta
     1021 tgtggccgag ccacaggtcg atttaccgtt ggtccagggc gaatatgatg tgccctcggc
     1081 cattggcgcg gccggcttgc tgactgttgt gcgcgatctg catttgcccg aattggctac
     1141 cggtgtggtt catctggtcg gcagccatat cgacgatgac cttttgtttt ttctggagca
     1201 gtccgatcag gtgccgtcgg ccgtggagat cggtgtgacg ctcaatgagg acggcagtgt
     1261 ggccgaggcc gggggcatcc tgattcagcc cctgccgccc tacgatccgg ccgccattgc
     1321 gcagctgaag gagcgcttgc aggagctgcc gcccatcgag gcgatgctgg ccgaagggaa
     1381 aaagcccgag caaatcctgg acgaggtgtt tgtggggatt acccacacgc tgttgtcgaa
     1441 gtacccggtg cggtttgaat gcaactgctc tcgcgagcag acgagggccc tgctcgtgtc
     1501 gttggggcgc gaggagctgg aagggattct tgagcatgag gggcaggccg tagtcaactg
     1561 ccagttctgc catgaggagt acgtcttcga ccgcgaagat ctattggaca tcctccagat
     1621 gatggaggaa gaagaatagt tttccggcca accggcgctt tggataatca gtaaaacgaa
     1681 accgcatggc ttctgccatg cggtttttgt ttctgtcggg gtggccggac tcgaaccggc
     1741 gacctcttcg acccgaacga agcgcgctac caggctgcgc cacaccccga acgaagaaga
     1801 ttatacgcgg ggttggggga agtggcaagt ggtagttgag gcctgacacg gccggcataa
     1861 agcggccaat gggcgatcgg cgtgatacaa ttaagccttg cggctatcgg ccgtgtatgt
     1921 acaggtaaag aaggacatac gattgaagtc gagaaatctg cgattgctaa tcggcgtgat
     1981 catcagcgcc gtttttattt ggctggcgtt acgcgggctg catctgggtg acgtatgggc
     2041 cagcctgcgc gcggccgact actggtggct gattcccagc gtcgccgtct actttctggc
     2101 cgtctgggcc cgcacatggc gatgggatta tctgctgcgg ccgatgaaac acatctcctt
     2161 gcgccgcctg tttccggtcg tcgtcatcgg ctacatgggc aacaatgtct accctttccg
     2221 ggcgggcgag cttttgcgag cctacgtcct tcgccaacaa gagggcgttt ccatgagcag
     2281 cagcctggcg accatcgtcg tcgaacgcgt cttcgacgga ctggttatgt tgctgttcgt
     2341 gttcgttgcc ctgccgttcg ccccattacc cagcgacagc attcgcttta tcgtcatgat
     2401 cggcagtgtg gcttttctgg gtgcgctgct tgtcttcttt gcggtcgccg ccgtgcccgc
     2461 gcgcttcatg gctctcaccg aatggtgcgc caaccgcttt ttgccggaac ggtttcgcgc
     2521 gcccatcatc gacttcgccc agcgtttcat cgtcggtctg gcttccttgc gcagcggccg
     2581 cggcctgatg atgatcttcc tgacctctgt ggtcatctgg ttgctggaga cagtgaaata
     2641 ctggtttgtc atgcacgcct ttgacttcac cgtgtcgttc ttcgccctga tgcttatgaa
     2701 cggcgtggtt aatctggcga caaccctgcc ttcggctccc ggctacatcg gcacctttga
     2761 tgggccgggc atcgccgtgc tgacgctcta tggtgtggat ccggccactg ccgcggccta
     2821 tactctgacg ctccacgccg cgctttggct gcccgtcacg ctgctgggca tctattacat
     2881 gatccgaacc gagttgggat ggggcgatat cggccgcgct gtcgatgccg ggcgcgagat
     2941 ggcggaacat ccgaatgagg ttgcggagcc acataaagag gtgagtgcgt gaaatttgcc
     3001 attatcgggg cgggcattgc cgggctgtcg gccgcctacg acttgttgcg ggccggtcac
     3061 caggtgaccc tgctggaggg tgacaatcgt accggcggcc tggctaccgg cttccaggat
     3121 gagcagtggg agtggccgtt ggagaagttc taccaccacc tgttcacctc cgatagcgag
     3181 atcatcggcc tggtcgagga gatcggcttg aaggacaagc tgttcttccc gcggccggtc
     3241 acttccgtca tctatcagga tcgcatcgtc cctttcgact caccgaagcg atggatcact
     3301 ttccccgggt tcaatttgct cgacgtcgct cgcttcggca cggtcagcgc ctatttgcgc
     3361 ttcaccagtc cgtggcgaga gttggagcag catctggccg acgactggct ccggcgttat
     3421 tatggcgaca agatttacga gatgatctgg cggccgatgc tcatcggcaa gttcggcccc
     3481 tattatcgcg acgtcaacat ggcctggatg tgggcgcgtc tccacgtgcg cagcccgcgc
     3541 ctgggctatt tcgagggcgg tttccaggct ttcatcgatc gtctgaccga agttgtgggc
     3601 gaactgggcg gcgaggtacg cctgaactgc cccgccgagc agatccgccc ggccgaagat
     3661 ggcggggttg aggtgacggc cggcggcaag acggagaagt acgacgcctg catcacgacg
     3721 acatcgccgg gcttgctggc ccgcatggct cccgatctgg ccggggacta tctcggtcaa
     3781 ctgctgaagc tggacagcat gggcgcggtc gtgctgaccc tggcgctgaa ggagtcgctc
     3841 ctggcggaaa gcggcacata ctggctcaac atcccggcca cctcgcccga caaggccggg
     3901 ggtgtgccgt tcctggcgct cgttgagcat accaactaca tcgaccgttc ccactacggc
     3961 ggcgaccaca ttatttattg cggcgattac gtgacacccg accaccccta tctgacgatg
     4021 gatcaggcgg aactggagaa gctgtttgtc ggtgttcttc atcgtttcaa cccgaacttc
     4081 cggcccgaat gggtgcgcaa gagttggctt ttccgcgctc gctatgccca gcccgtgccc
     4141 aaactcaatc actcgcagct tgtccctgat ttgcgcacgc cgatgtccgg gctttatttc
     4201 gccagcatga gccaggtcta tccctgggat cgcggcacga atttcgcggt ggagatcggc
     4261 cggcgggtag cgcgcctggc gctggccgac gcggccgatg ggcggctatc gccgcgtggt
     4321 gaaaacggtc gctaaacgct tcctttccgt cgatcatgag acttggagcg aggcatgcga
     4381 ttgattacaa tagggcgtaa tcgagggcct attctctatt gcctgatacg tttaaacgtg
     4441 cttgtcactt acttgcggcc atggtttgaa tcgcgattga caaccaaaaa tggacgcaca
     4501 aattcaaatt acgccacttg ggattttacg gcgtaaggag atcctaatat gaaaattgtt
     4561 gtgatcggta ccggctacgt aggtctgccc cacgcggctg ttctctctga atacgggcat
     4621 gaggttcatg cttacgacat cgacgaaacg cgtattgccg cctatcaatc gggcgaagcc
     4681 ggtaatatcg agcgttatgt caatgagccg ggcctggctg atattgtcgc cgagaatgta
     4741 aaccgctatc tattcttcac caccgacgtc accggtgtgg tggaagaaac cgacgtgttn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ggggttccta cgtcagcaac
     4921 ggcgccggcg gcagctgctt cggcaaggac attcagtcgt tgatctacca attgaatcat
     4981 gccgggcgcc caaccgactt gctccgcgcg gtttataaca tcaacgaata ccagaagatc
     5041 tatttgatcg accgcgccat ccacgaggcc catttcaact ttaatcagaa aaaagtcgcc
     5101 ctgctgggtc tgtcgttcaa gaagcgcacc aacgacatgc gcgattcggc cgccttgcgt
     5161 gtggcggagt cgctattgtc gcgtggcgtg gcgacgatcc aggcttatga tcccctggcg
     5221 atggacgaag ctcgccgtaa ttggttcaac ccggagaaga actatctcta tgagcgaatt
     5281 acctatcatg attcggccgt tgaggcgatc gaagggacgg acgctctcta tatttccacc
     5341 gactgggaag agttccgcgg cctggcgcgc acgatcagaa cgacagtgaa gccgccgtac
     5401 ctggtgattg atggccggcg catgttgcct gatttcgaga atcttgtggc tgcgggctat
     5461 gactatttgc cggtcggcgg cctgctcatg cgcggccgga gcaattcgag cgaagcctac
     5521 agcgagaacg accaggccat cccggcaatc gatgaactcc cgcagcaagt tccggcctga
     5581 cccccaaggc tctaaaatga tcgaggcttt acaactgtaa cgtttagagt atactagggc
     5641 gttaatcttt atggggagat cttcgccgct caacgcagcg gtttttccat atacaggaag
     5701 tagaagcatg ttgcagaaaa aaccaacgct cctgacacag gaaggactgg agcgactcac
     5761 taaggaattg gaacatttgc gcaccgtcgg ccgcgaggaa gtggcggagc gcttgcatca
     5821 ggctttccag gacggccagg acgacgattt cgtcgacaac gctgagctgg aagctgctcg
     5881 caacgcccag gcttttctgg aagggcgcat ccaggaactg gatgagattc tcaataacta
     5941 ccagatcatc accgaagata tggcccccca cgaccgcgtg aagctgggcg actgggtgac
     6001 tgtaaccgag gttggcgccg acgaactcga acgctatcaa ctggtcgggc cgcttgaggc
     6061 tgatcccgtg gccgggcgca tctccaacga aagcccgttg ggcaggttgc tggtgggcgt
     6121 taaggtgggc gaagtggtgc gcgtcaatgc gccacgtggc atcactgagt ttcgggtggt
     6181 tcaggtcgag taggcgacac cccgcaatca ggatcataaa tgcccgtgtt gcgccgttga
     6241 cgcgcccacg ggcttttttt tggagaaagt cgatgagttg ggaaccgaat ccgcttgagg
     6301 aacaacgtct ggagaagctg gcccgtttgc aggatgaggg tatcgatccc tatccgttgc
     6361 gggtggagcg aacgcacacg aactccgagg cgatcgccac attcgaggcg gccgatgagg
     6421 gcgtgcccgt gcctgtcacg gtctgcggcc ggctgattag cgtccgcgat atgggcaaga
     6481 cgatcttcgc ccacatcgcc gacgaggacg gtcgcctgca actgttcgtg cgtcgcgagg
     6541 acgtgggcga ggaatcccac gccgtcttca agaagctgct cgacctgggc gactttgtgc
     6601 aggctagtgg cgagatgttc cgcaccaagc tgggcgaagt cagccttcac gtgcgtgact
     6661 ggcgcatcct gagtaaggcc attagcccgt tgccggttgc caaggagcag gaagtggacg
     6721 gcaagaccgt ccgctatagc gccttcgcgg acatggaaga gcgctatcgc cagcgttatg
     6781 ccgacctggc ggtcaatcat gaggtgcgtg acatcttccg caaacgagcg cgcctgttta
     6841 gcgcgctgcg tcgttttatg gacgacaacg gctttctgga agttgagacc cctatcctgc
     6901 aaccgcttta tggcggcgcg gcggcgcggc cgttcacgac ctatcacaat cagctcaagc
     6961 aggagcttta cctgcgcatc tcattcgagc tgtacctgaa gcgcctgttg gtgggtggaa
     7021 ttgaacgcgt ctatgagatc ggccgcgact tccgcaacga gggcgtcagc ttcaagcaca
     7081 acccggagtt cacccaactg gagttttacg ctgcctattg ggattatcgg gacgtgatgg
     7141 atttcacgga gcggatggtc gcttatgccg ccgaggctgt gctaggcacg tccgatgttt
     7201 cctatcatgg ccatacgatc catcttggcg cgccctggat gcggctgacc atgcgcgagg
     7261 cgatcaaaca gttcgcagag atcgattaca tggactaccc cgacgcgccg gcgctggcca
     7321 aggccattaa agggattgga ggtgacgctc cgcccgacgc gccatggggc aagctgatcg
     7381 atggtctgct gggcgatttc gtcgagccac acctcattca gccgacgatc atcatggagt
     7441 acccgcgcga catctcgccg ctggccaagg gtgtgcccgg cgatccgctg catgtggagc
     7501 ggtttgagtt cttcattggc ggcatggaga tgggcaacgc cttcaccgaa ctgaacgatc
     7561 cgctcgagca gcgcaagcgc tttgaggcgt tgcagacgac cttcgccaag gaagacgaag
     7621 agcttaatcc gattgacgag gattatctgc gggcgatgcg ttatgggatg ccacccaacg
     7681 gtggctttgg catgggcctc gaccggctgg cgatgctcct gaccgacagc cggactctgc
     7741 gcgaggtctt gctcttcccg catctgcgcg atcgggaaga ttaacgctgt taccgcacag
     7801 tcgcatcaag ccggttgttt cagcttttcc tggaacgact tgcggaattt gcgcaccttc
     7861 ggatcaatga cataggcgca atagggttga ttgggattat tgacataata atcctgatga
     7921 tacccctcag cctcatagaa cacatcgaag ggaacgatct cggtcacaat gggattgggc
     7981 cacaccttgg cggccgcggt ttcatcgcgg atgcgttcgg cggccgcctt ctgcgcttcg
     8041 tcgtggtaga agatgaccga acggtattgc gggccgatgt cgttgccctg ccggttggga
     8101 gtcgtcgggt cgtggatgcg ccagaagatg tagagaatct cgtcgtagga gataaccgcc
     8161 ggatcgaacc acacctgcac cacctcagca tggccggtat gtttgccgca gacctgctca
     8221 taggttgggt tggcgacgtg gccacccgaa tagcccgaca ccacccgatc cacgccctga
     8281 acgtcgcgat agatggcttc gagacaccag aagcatcccc cacccaaggt tgctacttcg
     8341 agcttgttat ttgacataag taatcctgct cctctgcctg attcaaccag ggccgctttg
     8401 atgttcattg attatacgac ccgctgcaag atatgatagc aagcgggtgg
//
LOCUS       DAOMQG010000122         8316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3690_length_8316_cov_9.378284, whole genome shotgun sequence.
ACCESSION   DAOMQG010000122 DAOMQG010000000
VERSION     DAOMQG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_8316_cov_9.378284"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..931
                     /locus_tag="PLC06_08345"
     CDS             164..931
                     /locus_tag="PLC06_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRO90492.1"
                     /translation="MNEHEFPILEYDHSPDAMIEPSRILSPIDIPQHCVLCFFQDVIS
                     NLREQGALRIIYMLGSEMGPNPVYTLETEGRSVALMHPGVGAPLAGGFTDELIALGCR
                     KFIACGGAGVLDRSLVVGHLVVPNAAVRDEGTSYHYLPPSREITPSLEAVTVIEATLR
                     RHNVPYVLGKTWTTDGFYRETAARTARRRAEGCLTVEMEAAALFAVGRFRGVTMGQIL
                     YGGDDLSGDIWDDREWRAQQSIREQLFWLAAEACLML"
     gene            951..1307
                     /locus_tag="PLC06_08350"
     CDS             951..1307
                     /locus_tag="PLC06_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007321596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRO90493.1"
                     /translation="MPDQPTPIEAYIGTFPADVQTVLETIRRTIRAAAPDAVEAIAYG
                     MPAYKLNGKPLVYFGGFRNHIGLYATPTGHAAFAGELSKYKQGKGSVQFPLSEPMPLD
                     LITRMVQFRVNELSAS"
     gene            1406..1804
                     /locus_tag="PLC06_08355"
     CDS             1406..1804
                     /locus_tag="PLC06_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRO90494.1"
                     /translation="METNNDITAYHDAQTDEDRAICDLLRREIDRGLPEAESKVWHSH
                     PVWFLDGNPTVGYSKQKSGVRLMFWSGADFDEPGLRPGTGKHKDASIFYQSADQINLE
                     DLARWLEKSRQIQWDYKNIVKRKGVLERIK"
     gene            1937..2221
                     /locus_tag="PLC06_08360"
     CDS             1937..2221
                     /locus_tag="PLC06_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRO90495.1"
                     /translation="MTIQADLHLGAREARAQFADLLGRVYYRGDTVIVERSGKPMVAV
                     IPIELYEQLVAEREARFAVLDRIRENLPDIAEDEVLADVAEAVDAVRAER"
     gene            2240..2656
                     /locus_tag="PLC06_08365"
     CDS             2240..2656
                     /locus_tag="PLC06_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRO90496.1"
                     /translation="MIFDTNLFISSLLVRNGLPAQALEAWRSRRFILVTSPAIMAEVE
                     AALNYPRIRRKYHLGEEEIIGLFELLGTDALVVPGTADVTGGVPDDPDDEIVLAGAID
                     GEVDLIISGDRHLLSLKTFRDIPIITIRELLERLAG"
     gene            complement(2681..5059)
                     /locus_tag="PLC06_08370"
     CDS             complement(2681..5059)
                     /locus_tag="PLC06_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRO90497.1"
                     /translation="MIIGMDFGTTNSGMATFDGRAVDVLPLDPTAPNPRVARTALYIT
                     NEQAVTIGRAAVDRYFEHNIGRPMKMQKVWVGEIEVVGAEMYFVRDVYVFADALSPGR
                     LFLSIKTGLRDVNYPGTVIGQFWYSLEDLIALYLTVTRARAERLLGQEVRQVVLGRPV
                     HFAADEAGDRLAEARLLHAAFRAGYETVYLQYEPVAAAHSYALGLARPENALVFDFGG
                     GTLDITVMRLGEGRPRVLATGGIPIAGDVFDQKLVRAKLPRHFGEGSFYGPRHKALTV
                     PHWIYDSFADWQTILQLQSAENKQILREIAQTAQRKHQIEALLALVSGNYGLKMFDVV
                     EGAKRVLSEKRGAEIALEGPGFNVREFVTRGEFEGLIRGEARAIEQHLLETVAASGLR
                     PEQIDIVIRTGGSALIPVFHEMLGRHFGEAKVRTIDTFSSVTAGLGVIGHRLEQGEID
                     LHAHTPADFADLPPVTGGGLKVRPVNLDLLQRRIRLEEQGGRGEGEKGSGGAERRMLV
                     LLSRATTGENARADIIATSDDAAIPADLSALPIISALRVPPDNQLLLITSRYRFLLVT
                     ARQLAELREAGLDIENIHRFAARETIVAITDWAATREQERLLLVTSTGYARAYPLDSL
                     RPAVEAPVPLTFDDPPPGVPVLAQGVERGMDAVIVTEGGRGVRWPLSKIPLTGIQALN
                     PGRDGAFDRVTAALAVAPDDEVILLLDDGYARRMKAAWVSEAPRANAKARALVVRKAA
                     AVGLCGTGALTTVTNRRLVAADGNALPLEDSTKAFRLAKLEEEELVTAVVRG"
     gene            complement(5150..6016)
                     /gene="aroE"
                     /locus_tag="PLC06_08375"
     CDS             complement(5150..6016)
                     /gene="aroE"
                     /locus_tag="PLC06_08375"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRO90498.1"
                     /translation="MIDGNTQLIGLIGYPVAHSFSPLMHNAAAAALGLNVAYVPLPVR
                     PDELPDALRGLVALGFRGVNVTVPHKEAVMAHLDTIYPATRATGAVNTIVISDGRLTG
                     FNTDWSGFLSDLESHRLALYGRDCLVLGAGGSARSVVYALLRRGGRVTVLTRRPEQAQ
                     QLAAEIGPAFPMAAMVAVGRLEDAPRVAANLDEPVIINTTPLGMSGGLIDQSPWPDGA
                     PFPPGTFVYDLVYNPAETRFMAQARAAGRRAVNGLGMLVGQAVEAFEVMTSRRPDPAV
                     MRRAVETRGSGA"
     gene            complement(6013..6648)
                     /locus_tag="PLC06_08380"
     CDS             complement(6013..6648)
                     /locus_tag="PLC06_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRO90499.1"
                     /translation="MDQQEVLQIADDLRGIAAFGLNFARAGPHDEERWQLVLTAAARL
                     AALAGGEQTAGDLLAHYRANHFDIGPLASGEAVVIRDGKLLLVRRTDDGLWALPGGIT
                     DPGETLAQSARRELLEEAGLTGRVTRLLGVFDSRLWHSAKKIHFYHAVFLIETDDPEP
                     RPSPEVSAAAYFGEDELPPLSPGHHLRAPFIFQQLRGDAPIPYFDPPQGDL"
     gene            complement(6652..7392)
                     /locus_tag="PLC06_08385"
     CDS             complement(6652..7392)
                     /locus_tag="PLC06_08385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRO90500.1"
                     /translation="MHRFFVTPDQIRHHLVQFDADQAHQMRRVLRLRPGDRVVALDGL
                     GRQYEVTLEEVANARATGRAADPVAATGESSARLTLYQSLTRREKFEWVLQKGTEIGI
                     AAFVPVITRRSLVRDAEDVGPEKLGRWQRIIREAAEQSNRGLLPTLSPPLAFADALVA
                     ARRMDASLIARADELHRTIGDALGGRPAMAEIALFIGPEGGYDEEEVRLAESWGVVPV
                     SLGSRILRTETAAIVGAALVLHQLGELG"
     gene            complement(7446..8285)
                     /locus_tag="PLC06_08390"
     CDS             complement(7446..8285)
                     /locus_tag="PLC06_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90501.1"
                     /translation="MIIDETAVDTVVDAIKQSRKYRDTSEETIRQLAVEAVIEHKKPK
                     PAEKAVRKRLHSIMAPYLGDPDYAAARQLLTDSFAGGDEKVIRAACRDLMYTHLSTRE
                     RLPILDTFYRDIFAVTGPPRRLLDIACGLNPLAFPWMGLPAAGTDFIAYDIHEPRVDF
                     LNHYFVLQGLPPHAYVKDVAVGAPTESGDVALFLKEMPRFERNYPGHGRALLDAIDTH
                     WLVVSFPTISTHGGRNLTNRYREFFQQMIDGSGWSAVELLFDTELVFCAEKTTDGRPP
                     TAG"
BASE COUNT         1579 a         2598 c         2679 g         1460 t
ORIGIN      
        1 cgccccgatg ggacgtttga gcttccgccg ttttagctcc ccgccggatt gaatcctgcg
       61 ctttaccttg ccaatcgcgt caggacccgg caaggacgcc cggcattcac tcaggacgat
      121 taccccacgc cggaagcgaa agatcacagg tgactcctga gctatgaacg aacacgaatt
      181 ccctatcctg gaatatgacc acagccccga tgcaatgatc gagccatcga ggatactttc
      241 gcccatcgac atcccgcaac actgcgtgtt gtgcttcttc caggacgtca tcagcaacct
      301 gcgcgagcag ggggcattgc gcatcatcta tatgctcggt tcggagatgg ggccgaaccc
      361 ggtctacacg ctggaaacag aaggacgatc ggttgcgctc atgcatcccg gcgtcggtgc
      421 gcccctggcc ggcggcttca ccgacgagtt aatcgccctc ggctgccgaa aattcatcgc
      481 ttgcggcggc gcgggcgtgc tggatcgaag cctcgtagtc ggccatcttg tcgtgcccaa
      541 cgccgccgtg cgcgacgaag gaacttccta tcattacctg ccaccctcgc gcgagatcac
      601 tccatcactg gaagcggtaa cggtcatcga agccacgctc aggcgccaca acgtgcccta
      661 cgtgctcggg aagacgtgga cgaccgatgg cttctaccgg gagacagccg cgcggaccgc
      721 gcgacggcgg gccgagggct gtctaacggt ggagatggaa gcggccgcgc tctttgccgt
      781 cggccgattt cgcggcgtca caatgggaca gatcctctat ggtggcgacg atctgagcgg
      841 ggatatatgg gacgaccgcg agtggcgcgc ccagcaatcc atccgtgagc aactgttttg
      901 gctggcggcc gaggcatgtt taatgcttta gtgcaatgta taatagtccc atgcccgatc
      961 aaccaacccc catagaagcc tatatcggaa cattccccgc ggacgtccaa acggtactcg
     1021 aaacgattcg ccggacgata cgcgcggccg cccccgacgc cgtggaagcc atcgcctatg
     1081 gcatgccggc ctataagcta aatggcaaac cgctggtcta tttcggcggc ttcaggaacc
     1141 acattgggct atatgccaca ccaaccgggc atgctgcttt cgccggcgag ctgtcgaagt
     1201 acaagcaagg caaaggctcg gtccaattcc cgttgagcga gccgatgccg ctcgacctca
     1261 tcacccgaat ggtacaattt cgagtgaatg aactgagcgc atcataatcc tgccccgctt
     1321 gtagtaaggc gctttagcgc cgtctccggg gacgtaacta aagttgctga ctacaaacaa
     1381 aaaacactat gaactgagag gcaacatgga aacaaacaac gatatcactg cctaccatga
     1441 cgcgcagacg gacgaggaca gggcgatctg cgatctgctg cggcgggaaa tcgaccgcgg
     1501 cttgccggaa gcggagagca aggtctggca cagccatccc gtctggtttc tggacggcaa
     1561 cccgacggtc ggctacagca agcagaaaag cggcgtgcgg ctgatgtttt ggagcggggc
     1621 cgacttcgat gagccggggc ttaggcccgg cacgggcaag cacaaagacg cttcgatctt
     1681 ctaccaatcc gccgatcaga tcaatttaga agatctcgcc cgctggctcg aaaaatcgcg
     1741 gcaaatccag tgggattaca agaacatcgt caagcgcaaa ggcgtcctgg agaggataaa
     1801 gtaaatacgg tcaggcagtg acctgttaat gggcgaccaa accctatagt atggcataca
     1861 atgcctattg tgtacacggt tgacattgtg ctaccttgtc gttatcattg ccccatattg
     1921 atggagcgtt atcgcaatga ctatacaagc cgatctacac ttgggggcca gggaagcgcg
     1981 cgcgcaattt gccgacctgc tcggccgcgt ttactatcga ggcgacaccg tgattgtcga
     2041 gcgttcgggc aagccgatgg tggctgtcat tccaattgag ttatatgagc aactggttgc
     2101 cgaacgagaa gccaggttcg cagtactcga tcggattcgc gagaaccttc ccgacattgc
     2161 ggaagacgag gttttagccg atgtggccga ggctgttgat gccgtgcgcg ctgaacgcta
     2221 ggcccacatc gtgcttcgcg tgatcttcga tacaaacctc ttcatcagca gcttgctcgt
     2281 gaggaatggg ttgcccgcac aggctttgga ggcctggcga agtcgtcgtt ttatcctcgt
     2341 cacatcgcct gccattatgg ctgaggtcga agccgccctg aactacccgc gcatccgccg
     2401 caaatatcat cttggtgaag aggaaattat cggattattc gaattattag ggacggacgc
     2461 gctggttgtg ccgggaactg cggatgtgac aggaggcgtt cccgacgatc cggatgatga
     2521 aatcgttctc gccggcgcta tagacggaga agtcgatctc atcatcagcg gcgatcggca
     2581 tcttctgtca ctaaaaacat ttcgtgacat acccatcatc accattcggg aattactgga
     2641 acgattagct ggttgacagc gccattaggc tgaacatccc tcaaccccgc acgacggccg
     2701 tcaccaactc ctcctcctcc agcttggcca aacgaaacgc cttcgtcgaa tcttccaggg
     2761 gcagcgcatt gccgtcggcc gccaccagtc gccggttggt gaccgtcgtc agcgcgcctg
     2821 tgccgcacag tccgaccgcc gccgccttcc ggacaaccag cgccctggct ttggcgttcg
     2881 cccgcggcgc ttcggaaacc catgcggcct tcatgcggcg agcgtagccg tcatcgagca
     2941 acaggataac ctcgtcgtcc ggcgcgacgg ccagcgccgc cgtcacccga tcaaacgccc
     3001 cgtcccggcc ggggttcagc gcctggatgc ctgtcagggg aatcttcgac agcggccaac
     3061 gcacgccgcg gccgccctcg gtgacgatga cggcgtccat gccgcgctcc acaccttgag
     3121 ccagcaccgg cacgccgggc ggcgggtcgt cgaatgtcag tggcaccggc gcctccacag
     3181 caggtcgcaa ggaatcgagc gggtaggccc gagcatagcc ggtggacgtc accagcagca
     3241 gccgctcctg ctcgcgggtc gcggcccagt cggtgatggc gacgatggtc tcgcgagcgg
     3301 cgaaacgatg aatgttctca atgtcgaggc ccgcttcgcg cagttcggcc agctgccgcg
     3361 ccgtcaccag caggaagcgg tagcgggagg tgatcagcag cagctggttg tccggcggca
     3421 cgcggagcgc ggagataatc ggcagggcgg acaggtcggc ggggatggcc gcgtcgtcgc
     3481 tcgttgcgat gatgtcggcc cgcgcattct cgcccgtggt cgctcgactc agcagcacca
     3541 gcatccgcct ctccgctcct ccgctccctt tctccccttc tcccctgccc ccctgctcct
     3601 ccagccgaat ccgccgctgc agcagatcca ggttcaccgg ccggaccttc aggccgccgc
     3661 cggtcaccgg tggcaggtcg gcgaaatcgg ccggggtgtg ggcgtgcaga tcgatttcgc
     3721 cctgctccag ccggtggccg ataacgccta gcccggcggt gacgctgctg aaggtatcga
     3781 tggtgcgcac cttcgcttcg ccgaaatggc ggccgagcat ctcatggaac acagggatga
     3841 gcgccgagcc gccggtacgg atgacgatgt cgatctgctc cggccgcaag cccgacgcgg
     3901 ccaccgtttc cagcaagtgt tgttcaatgg cgcgcgcttc cccgcgaatc aggccttcga
     3961 actcgccgcg agtgacgaac tcgcgcacgt tgaagcccgg cccttcgaga gcgatctcgg
     4021 ccccgcgctt ctccgacagc acccgcttcg cgccctcgac aacatcgaac atcttcaggc
     4081 catagttgcc cgacaccagc gccagaagcg cctcgatctg atgcttgcgt tgggcggtct
     4141 gggcgatctc gcgcaggatt tgcttgttct cggccgactg gagttgcagg atggtttgcc
     4201 aatcggcgaa ggagtcgtag atccaatgcg gtacggtcag ggccttgtga cgcggcccat
     4261 agaaactgcc ctcgccgaag tggcgcggca gcttggcgcg gacgagcttc tggtcgaaga
     4321 cgtcgccggc gatggggatg ccgccggtgg ccagcacgcg cggccgcccc tcgcccaggc
     4381 gcatgacggt gatgtcgagc gtgccgccgc caaagtcgaa cacgagcgcg ttttccggcc
     4441 gggccagccc cagcgcgtag ctatgggcgg cggccaccgg ctcgtattgc aggtagaccg
     4501 tctcgtagcc ggcgcggaag gcggcatgaa gcaggcgcgc ctcggccagc cggtcgccgg
     4561 cctcgtcggc ggcgaagtgg acggggcgac ccagcaccac ctgccgaacc tcttgcccca
     4621 gcagccgctc ggcgcgggcg cgggtgacgg tcaggtagag ggcgatcagg tcttcgagcg
     4681 aataccagaa ttggccgatg accgtgccgg gataattgac gtcgcgcagc ccggttttaa
     4741 tcgacaggaa aaggcggccg ggcgataggg catcggcaaa gacgtagacg tcgcggacga
     4801 aatacatttc ggcgccgacc acctcaattt cgccgaccca gaccttttgc atcttcatcg
     4861 ggcggccgat gttgtgctcg aaatagcgat cgacggcggc gcggccgata gtgacagcct
     4921 gctcattggt gatgtagagc gccgtgcggg ccacgcgcgg gttgggcgcc gtcgggtcga
     4981 ggggcaatac gtccacggcg cggccgtcga aggtcgccat gccgctattg gtcgtgccga
     5041 agtccatgcc gatgatcatg agagaagccc tcaacggaaa gggggagcat tatccacggg
     5101 ttgcatggat ctcgcaagtt cttcgtcacg atgccggaag cagcgatcgc tacgcgccgc
     5161 ttcctctcgt ctcaacggcg cgccgcatca ccgccggatc ggggcgacgg ctggtcatca
     5221 cctcgaacgc ctcgaccgcc tgccccacga gcatgcccag cccgttgacg gcgcgacggc
     5281 cggcagcgcg agcctgcgcc atgaagcgcg tctcggccgg attatagacg aggtcataga
     5341 cgaacgtgcc gggcgggaaa ggcgcgccat caggccaggg ggattgatca atcagcccgc
     5401 ccgacatgcc gagcggggtg gtgttgatga tcaccggctc gtccagattg gcggcgacgc
     5461 gcggcgcgtc ttcgagccgg cccacggcaa ccatggcagc catggggaag gccgggccga
     5521 tctccgcggc cagttgttgc gcctgctccg ggcggcgagt gaggaccgtc acgcggccgc
     5581 cccgccgcaa cagggcatag accaccgacc gcgcggatcc gccagcgccg agcaccaggc
     5641 aatcgcgccc atagagcgcc agccggtggc tttccagatc ggaaaggaag cccgaccaat
     5701 cggtgttgaa gccggtcagc cgtccgtcac tgataacgat cgtgttgacc gcgccggtgg
     5761 cgcgcgtcgc cggatagatg gtatcaagat gggccatcac cgcctctttg tgcggcacgg
     5821 tcacgttgac gccgcggaaa ccgagcgcca ccaggccgcg cagcgcgtcg ggcagctcat
     5881 ccggccggac cggcagcggc acgtaagcga cgttgaggcc gagcgcggcg gcggcggcgt
     5941 tgtgcatgag tgggctaaag ctgtgggcga ccggatagcc gatcagcccg atgagttgcg
     6001 tgttgccatc gatcataggt caccctgggg tgggtcgaaa taagggatgg gcgcgtcgcc
     6061 gcgcaactgt tgaaagataa aaggggcgcg cagatggtgg ccgggcgaga gcggcggcag
     6121 ttcatcctcg ccaaagtagg ccgcggccga cacttcgggc gacggtcgcg gctcgggatc
     6181 atccgtttcg atgagaaata cggcgtggta gaagtggatt ttcttggcgc tgtgccacag
     6241 gcgcgaatcg aagacgccca gcagccgggt cacgcggccg gtgaggccgg cctcttccag
     6301 cagctcgcgg cgagcggact gggccagcgt ctcgccggga tcggtgatgc cgccgggaag
     6361 cgcccacagg ccgtcgtcag tgcggcggac gagcagcagc ttgccgtcac ggatgacgac
     6421 cgcctcgccg ctggccaggg ggccgatgtc aaaatggttg gcgcgatagt gggccagaag
     6481 atcgccggcc gtttgttccc ccccggccag cgccgccagc cgggccgccg ccgtcaggac
     6541 gagctgccat cgctcctcgt cgtgggggcc ggcccgcgcg aagttgagac caaaggcggc
     6601 gatgccgcgc aggtcatcgg caatctgaag cacttcctgt tgatccacgc gctaccccaa
     6661 ctctcccagt tgatgcaaaa caagcgccgc gccgacgatg gcggccgtct ccgtgcgcag
     6721 aatgcggctg ccgagcgaca cggggacaac gccccatgac tcggccagtc gcacctcttc
     6781 ctcatcatag ccgccctcgg ggccgatgaa cagggcaatt tcggccatgg ccggccggcc
     6841 gcccaacgca tcgcctatgg tgcgatggag ctcgtccgcc cgagcgatca gactcgcgtc
     6901 cattcggcgg gcggcgacga gggcatcggc gaaggcgagc ggcggggaca gcgtcggcag
     6961 caggccccga ttcgactgtt cggccgcctc gcgaatgatg cgctgccagc ggccaagctt
     7021 ctccgggccg acgtcttcgg catcgcgcac cagcgagcgg cgggtgatga ccggcacgaa
     7081 ggcggcaatg ccgatctccg tgcccttctg caacacccat tcgaacttct cgcgccgggt
     7141 caggctctgg taaagggtca gccgggccga cgactcgccg gtggcggcga cggggtcggc
     7201 cgccctgccg gtggctcgcg cattggccac ctcttccagc gtcacctcgt attgtcgccc
     7261 cagcccgtcg agggcgacca cccgatcgcc cggtcgcaaa cgcagcacgc ggcgcatctg
     7321 atgggcctga tccgcgtcga actgcaccag atggtggcga atttgatcgg gggtgacgaa
     7381 gaagcggtgc atgatgtgac ggcggacgac tgacggctga ccgcggccaa actataaatg
     7441 tgaaactatc cggcggtcgg tggtcggccg tcggtggtct tctccgcaca aaagaccaac
     7501 tcagtgtcga acagcagctc gacggccgac cagccggagc cgtcgatcat ctgctggaaa
     7561 aactcgcgat agcgattggt cagattgcgg ccgccgtggg tgctgatggt gggaaacgag
     7621 acgaccagcc agtgcgtgtc gatggcgtcg agcaaggccc gcccgtggcc ggggtagttg
     7681 cgctcgaagc gcggcatctc cttcaggaac aaggcgacgt cgccgctctc ggttggtgcg
     7741 cccacggcca cgtccttgac gtaggcatgc ggcggcaagc cctgcaagac aaagtagtga
     7801 ttcaggaagt cgacccgcgg ctcgtggatg tcataggcaa tgaaatcagt cccggcggcg
     7861 ggtagtccca tccagggaaa ggccagcggg ttgaggccgc aggcgatatc gagcaggcgg
     7921 cgaggcggcc cggtgacggc gaagatgtca cgatagaagg tgtcgagaat gggcagccgt
     7981 tcgcgggtgg agagatgggt atacatcagg tcgcggcagg cggcgcggat cactttctcg
     8041 tctccaccgg cgaaactgtc ggtcagcagt tgtcgcgcgg cggcatagtc ggggtcgccc
     8101 agatagggag ccatgatgct gtggagtcgt ttgcggacag ctttctcggc cggtttgggc
     8161 tttttgtgct cgatcacggc ttcgacggcc aactggcgga tggtctcttc gctggtgtcg
     8221 cggtatttac gggactgttt gatggcgtca acgacagtgt cgacggccgt ttcgtcgata
     8281 atcataggct tgcctcgtat ggttgtatct attgta
//
LOCUS       DAOMQG010000123         8300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3699_length_8300_cov_7.243784, whole genome shotgun sequence.
ACCESSION   DAOMQG010000123 DAOMQG010000000
VERSION     DAOMQG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_8300_cov_7.243784"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1024)
                     /gene="pstB"
                     /locus_tag="PLC06_08395"
     CDS             complement(236..1024)
                     /gene="pstB"
                     /locus_tag="PLC06_08395"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRO90502.1"
                     /translation="MTHPNNNYAIEARDLNIYYGGFRAVKDINLQFRPRQITALIGPS
                     GCGKSTVLRAFNRMNDLIPAARAEGEVLFHGKNIYDPDVDPVEVRRRVGMVFQKPNPF
                     PKSIYENVAWGARINGFKGSKADMDALVEGSLRDAAVWDEVKDKLHQSGLSLSGGQQQ
                     RLCIARAIAIRPEIILMDEPASALDPISTLRIEELIQVLKDNYTILIVTHNMQQAARA
                     SDFTAFFNMDTDRAGYLVEYGPTDRLFTTPQHEMTEQYITGRFG"
     gene            complement(1037..2425)
                     /gene="pstA"
                     /locus_tag="PLC06_08400"
     CDS             complement(1037..2425)
                     /gene="pstA"
                     /locus_tag="PLC06_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRO90503.1"
                     /translation="MIGRETIADRVRADLFPEGEEANRAVHKRRRLGRVWRAIFIGST
                     AIAILILSILLVKILNDTMGLVAEQSEMPERGLLLAYDPGGATESLDDLAYDDLVTLY
                     AANVSAGRCRAVEREQRFHADRFVCDDPDKLAAACASAAPPGACTLAPRDESNLRQLI
                     RADVVRPRILATWNAIESILNREEILAEAAARFPDADVYFKSWLRWEFITSPQSSYPE
                     IAGIRTAILGTLWVVAIAVAFGFPLGVAAAIWLEEYADKRNRLTGIIQTNINNLAGVP
                     SIIYGLLGLAVFVRALEPLTSGALFGAVEAGTTANGRTILSAGLTLGLLGLPVIIISA
                     QEAIRAVPPSLRQASYGLGATRWQTVRHHVLPNAIGGILTGVILSMSRIIGETAPLVV
                     VGASTYITADPTGPFSKFTTLPSQIYQWTARPQDTFRDLAAATIVALLLLLLLLNAGA
                     IYLRNRHARRLG"
     gene            complement(2422..3402)
                     /gene="pstC"
                     /locus_tag="PLC06_08405"
     CDS             complement(2422..3402)
                     /gene="pstC"
                     /locus_tag="PLC06_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRO90504.1"
                     /translation="MNSDTCLDQRPAINNGASDTTPPADIAAQLRKRPRAGEAIIEGL
                     LFAAGVISILTTIGIVAVLVRDAVDFFLLPEVTLVEFLTHTIWQPQTGEFGVRPLLTA
                     TLVISAIAMLVGVPLGLFTAIYLSEYASPRFRATLKPVLEILAGIPTVVLGYFALTFV
                     TPLLRSIFGDDVVKIYNMASAGIVVGILIVPLIAGLSEDALHAVPDSLRQAAYGLGAT
                     RMETSLRVVLPAALSGISAALVVAASRAVGETMIVALAAGAGPNLTLNPFQSAETMTG
                     YMVRISGGDLSYGSIDYRSIFAVGLVVFALTLILNVISGRIVRRFREVYE"
     gene            complement(3904..4965)
                     /locus_tag="PLC06_08410"
     CDS             complement(3904..4965)
                     /locus_tag="PLC06_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRO90505.1"
                     /translation="MLKHMKHNEKIYMRFTVIGLLMTISLAAVACGGTGATPAQVAPT
                     VQAAVEQVAPTVEAAVADQTTDGLPMPDPLAVSGDIVTAGSSTVFPLTERIVERYEYE
                     GFAGQVTIDSIGTGAGFERFCVAGETDIANASRAIKDEEIASCAAIGRTPLEFRVGTD
                     ALAVVVNPANEFAQNLTLEQLALAFSTAETWADVDPSFPAEPIARYSPGTDSGTFDYF
                     VEAVFDEDSAPILAARNTQFSEDDNVLVQGVEGDIYAIGYFGYAYYLENADRLRIINV
                     EGVEPTAESVEDGSYPLARPLYIYSDGGIMQAKPQVADFINFYLTYVNEEIIDVGYFP
                     AGEDALNQARQNWPTGMGQ"
     assembly_gap    5054..5153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5308..7386)
                     /locus_tag="PLC06_08415"
     CDS             complement(5308..7386)
                     /locus_tag="PLC06_08415"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRO90506.1"
                     /translation="MNEQESTTTNREQTRHWLASILLRRGDMVIPRFARLYARLAERP
                     RGWRRQLRRKLAVTVTGAALLLAMAGMGAQFAARAETDNTITVVNGEVSVANNGKCSL
                     IEAINNANDTTDGVADNTDCAPGDPAGADVIQLPAGGLFSVSKIMADYSYGYTALPVI
                     TSTITVEGNGSTINRTGKKDMRFFTAAPYSEGNVPNLTLNDLTLTNGKNAYYQGGAVY
                     AYQAKLTLNNCTITGNGTDGNGGGVFAAYTDLTISDSTISNNSSYGGGGVYAGYGSIT
                     IVDSTISDNKADGYGTGGGLYLNNAIVTISNVSIDGNRAMTGSGMMINYSDLSLTDST
                     ITNNSGKGDYSYGGGFYFMDVTGTINRVSITGNEAEYGGGAFVGGGSLAIKSSTLSGN
                     SANMNGGAIQVDSATPLLVVNSTLSGNSAGATGGGLYSRGTATLVNSTVTGNSAGEAG
                     GGAQVIGGSLTLQRTLLAGNTAPVGREVNRTAGTVTADSHNLFGAGGNAGLAGFAAGA
                     TDVVPAVGLAAIVGPLADNTGQTLTHALPANSPAIDKGPSAACAADPVNGLDQRSQPR
                     NVNGNGAASANECDIGAYEFSSGGPIETPTATATPLVTPTRTPTATATTVVITPKPSL
                     TPTATGTAGPSPTATATGTAGPSPTPTATTQRPTVTPTATSTAGPSPTATATGEPGGE
                     AILLPVVLRP"
     gene            complement(7722..>8300)
                     /locus_tag="PLC06_08420"
     CDS             complement(7722..>8300)
                     /locus_tag="PLC06_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90507.1"
                     /translation="AITSPAAQQAGNFWYGDYIPAAKLPTKSVTGRWYLQSSGTKNHI
                     PRALVLYPTYADPVNTWVNIWNTYDAAEAEVYWDTALGWTPTQVITESIAPPNGPTTF
                     NVELALVDNDKDARPVWVTVEAGGVTQTQKPNNPSDGEQLNLMTFTLDVPEGTDMITI
                     TVYSPSAALDDVIGDSAALVGMRANYMCAPLN"
BASE COUNT         1348 a         2432 c         2793 g         1627 t
ORIGIN      
        1 cgtcaatcat ccgctgcgcc gtcaccaggt cgcgctccac gaaggcatag gtcatcttga
       61 ggatgtggtc ttgcacctcg ctgcccatgc gcagcacttc atcctgcaat cgttgtagct
      121 ctcgatcaaa tgtagggcgc ataatcagcc tcctgatcct tcattcctga ttcgcgttta
      181 gaaaccgggt ttctcggaag aaacccggtt tctaccgtaa ttcgtaattc gtaaattacc
      241 caaaccgtcc cgtaatatac tgctccgtca tctcatgctg cggcgtcgtg aacaggcggt
      301 cggtggggcc atattccacc agatagccgg cgcggtcggt gtccatgttg aagaaggcgg
      361 tgaagtccga ggcgcgtgcc gcttgctgca tgttgtgggt aacgatgagg atggtgtagt
      421 tgtccttcag cacctggatc agctcctcga tgcgcagtgt ggagatgggg tcaagggcgg
      481 aagcgggttc gtccatcagg atgatctccg ggcggatggc gatggcgcgg gcgatgcaca
      541 gccgctgctg ctggccgccg gaaagggaca ggccgctctg atgcagcttg tccttcacct
      601 cgtcccacac ggccgcgtcg cgcagcgatc cctcgaccag cgcgtccatg tcggccttgc
      661 tgcccttgaa gccgttgatg cgcgcgcccc aggccacatt ctcgtaaatg ctcttgggga
      721 acgggttcgg cttctggaag accataccca cgcggcggcg cacttccacc gggtccacgt
      781 cggggtcgta gatgttcttg ccgtggaaca gcacctcgcc ctcggcgcgg gcggcgggaa
      841 tgaggtcgtt catgcggttg aaggcgcgca gcacggtgct cttgccgcag cccgacgggc
      901 cgatgagggc ggtgatctgt cgcggccgaa attgcaggtt gatgtccttc acggcgcgga
      961 aaccgccgta gtagatgttg agatcgcggg cctcgatggc gtaattattg ttgggatgag
     1021 tcatctgaat cgtatcctag cccagccgcc gggcgtggcg attgcgcagg taaatggccc
     1081 cggcattgag cagcaggagc agcagcagca gggccacgat ggtggccgcg gccaggtcgc
     1141 ggaaggtgtc ctgcggccgc gccgtccatt ggtagatttg cgacggcagg gtggtgaatt
     1201 tggagaacgg gccggttggg tcggcggtga tgtaggtgga cgcgccgacg acgaccagcg
     1261 gggccgtctc gccgataatg cgcgacatgg acaggatgac gccggtcagg atgccgccga
     1321 tggcgttggg cagcacgtga tggcgcaccg tctgccagcg cgtggccccc agcccatagc
     1381 tcgcctgccg cagcgagggc ggcacggcgc ggatcgcttc ctgggcgctg atgatgatga
     1441 ccggcagccc cagcagcccc aacgtcagac cggcggagag gatggtgcgg ccgttggccg
     1501 tcgtgcccgc ctccaccgcg ccgaagagcg cgccgctggt cagcggctcc agcgcgcgca
     1561 cgaacacggc cagaccgagc aggccataga tgatcgacgg cacgccggcc agattattga
     1621 tgttggtctg gatgataccg gtcagccggt tgcgtttgtc ggcgtactcc tccagccaga
     1681 tggcggcggc cacgcccagc gggaagccga aggccacggc gatggccacc acccacagcg
     1741 tgcccagaat ggccgtgcga atgccagcga tttcggggta gctcgattgc gggctggtga
     1801 tgaactccca ccgcagccac gatttgaaat agacgtcggc gtcgggaaaa cgcgccgccg
     1861 cctcggccag aatctcctcc cgattgagga ttgattcgat ggcgttccag gtagccaata
     1921 ttcgcggccg cacgacgtcg gcgcggatga gctgccgcag gttgctctcg tcgcgcgggg
     1981 cgagggtgca ggcccccggc ggcgcggccg aggcgcaggc ggcggccagc ttgtcgggat
     2041 cgtcgcagac gaagcggtcg gcatggaaac gctgctcgcg ctccacagcc cggcagcggc
     2101 cggccgacac gttggcggca tagagggtga cgaggtcgtc ataggccagg tcgtcgagcg
     2161 attccgtcgc gccgccgggg tcgtaggcca gcagcagccc gcgctccggc atctcgctct
     2221 gctcggccac cagccccatc gtgtcgttga ggattttgac cagcaaaatc gagaggatga
     2281 gaatggcgat ggcggtggag ccgatgaaga tcgcccgcca gacgcgccca agccgccgcc
     2341 gcttgtggac ggcgcggttg gcttcctcgc cttcggggaa cagatcggcc cgaacccggt
     2401 cggcgatggt ctcccggccg atcattcgta cacctcgcgg aagcggcgca cgattcggcc
     2461 gctgatgacg ttgaggatga gggtcagggc gaacacgaca aggccgacgg cgaagatgct
     2521 ccggtagtcg atggagccat agctgaggtc gccgccgctg atgcgtacca tgtagccggt
     2581 catcgtttcg gccgactgga acgggttgag ggtgaggttc ggcccggccc cggcggccag
     2641 ggcgacgatc atcgtctcgc ccacggcgcg cgaggcggcc acaaccagcg cggccgagat
     2701 gcccgacagc gccgccggca gcaccacccg caagctggtc tccatgcgcg tcgcgccgag
     2761 gccataggct gcctgccgca aggagtcggg cacggcgtgg agcgcgtcct cgctgaggcc
     2821 ggcgatgagc ggcacgatca ggatgccgac gacgattccg gccgaggcca tgttgtagat
     2881 cttcacgacg tcgtcgccga agatggagcg cagcagcggc gtgacgaagg tgagggcaaa
     2941 atagcccagc accacagtgg ggatgccggc caatatctcc agcacgggct tcagcgtggc
     3001 ccggaagcgg ggcgaggcgt attcgctcag gtagatggcg gtgaacaggc caagcggcac
     3061 gccgacgagc atggcgatgg cgctgatgac cagcgtggcc gtcagcagcg gccgaacgcc
     3121 gaattcgccc gtttgcggct gccagatggt gtgggtcagg aattcgacca gcgtgacttc
     3181 gggcagcagg aagaagtcga cggcatcgcg cacgaggacg gcgacgatgc cgattgtggt
     3241 gaggatggag atgacccccg cggcgaataa tagtccttcg atgatcgctt cgcccgcgcg
     3301 ggggcgcttt ctcagttggg cggcgatgtc ggcgggggga gtagtgtcgc ttgcgccgtt
     3361 gttgattgcc ggtcgctgat ccagacaggt gtcgctattc attggaactg tctcctcttc
     3421 cgctttgttc gtagtcgccg gctttagccg cgttcttctg ccttgttcgt agttagcggc
     3481 ttttgccgcg ttcttctgcc ttgttcgtag tcgtcggctt tagccgcgtt cttctgcctt
     3541 gttcgtagtc gtcggcttta gccgcgttct tctgccttgt tcgtagtcgt cggctttagc
     3601 cgcgttcttc tgctttgttc gtagttagcg gcttttgctt tttaacaaat taatgtggta
     3661 taggccgtag gggtgaggca gcggcgaaat caccccatct tgcagaaaca actcgtttcg
     3721 ccgctgcctc accctctttg cccggagggt caggcaaccg ggagcggagg ccgcgtgttt
     3781 cccagtgacg gccgccctgg ttgcctgacc cctaccccgc tataccgcat taatttataa
     3841 atgtgcaaaa gcgcttaact acgaacaaaa cgacagcgct gaagcgcttt actacaaaca
     3901 ggtctactga cccatacccg tcggccagtt ttggcgcgcc tgattcagcg catcctcgcc
     3961 ggcggggaag tagccgacgt cgatgatctc ctcattgacg taggtcagat agaagttgat
     4021 gaaatcggcc acctgcggct tggcctgcat aatgccgccg tcgctgtaga tgtagagcgg
     4081 gcgggcgagc gggtagctgc cgtcctcgac cgattcggcc gtcggctcca cgccctcgac
     4141 attgataatc cgcagccggt cggcgttctc cagatagtag gcgtagccga agtagccaat
     4201 ggcgtagata tcgccctcga cgccctgcac cagcacgttg tcgtcctcgc taaactgggt
     4261 gttgcgcgcg gccaggatag gggcgctgtc ctcgtcgaag acggcctcga cgaagtagtc
     4321 gaatgtgccg ctgtcggtgc cggggctata gcgggcgatt ggctcggccg ggaaggacgg
     4381 gtcgacgtcg gcccaggtct cggccgtgct gaaggccagc gccagttgtt ccagcgtcag
     4441 gttctgggcg aattcgttgg ccgggttcac gaccacggcc agggcgtcgg tgcccacgcg
     4501 gaactccagc ggggtgcggc cgatggccgc gcacgaggcg atctcttcgt ccttgatcgc
     4561 ccggctggcg ttggcgatgt cggtctcgcc ggccacacag aagcgctcga agcccgcgcc
     4621 ggtgccgatg ctgtcgatgg tgacctgccc ggcgaaccct tcatattcat agcgctcgac
     4681 gatgcgctcg gtcaatggga agacggtgga gctgccggcg gtgacgatgt cgccgctgac
     4741 agccagcggg tcgggcattg gcaagccgtc ggttgtttgg tcggcgacgg ccgcttcaac
     4801 ggtgggcgcg acttgctcca cggccgcctg aacggtgggg gcaacctggg ccggggttgc
     4861 gccggtgccg ccgcaggcga cggccgccag gctgattgtc atcaatagtc cgatgacggt
     4921 gaaccgcatg tatatcttct cgttgtgttt catgtgtttt aacatgaacg cgaggatagc
     4981 acacgactgt taaggcggaa gccgcgtgcg gttaagatcg ggctaaggtt cggttaacgg
     5041 gaggttaagg ggannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagccgc
     5161 gtgcggttaa gatcgggcta aggttcggtt aacgggaggt taaggggagt ggcgagcgga
     5221 ctttatagaa accgtttata gaaaccgggt ttctcggaag aaacccggtt tctatcggtg
     5281 ttctatcggt gttctatcgg tgtgctttta cgggcggagc acgacgggca gcaggatggc
     5341 ctcgccgccg ggttcgccgg tggcggtcgc tgtgggcgag gggccggccg tgctggtggc
     5401 cgtcggcgtg acggtgggtc gctgagtggt ggcggtcggc gtgggcgagg ggccggccgt
     5461 gccggtggcg gtcgctgtgg gcgaggggcc ggccgtgccg gtagcggttg gggtcagcga
     5521 aggcttcggc gtgatcacca cagtggtggc ggtggcggtt ggcgtccgcg tgggcgtcac
     5581 caatggcgtg gctgtggccg tcggcgtctc gattggcccg ccggacgaga actcataggc
     5641 gccgatgtcg cattcgttgg ccgaggccgc cccgttgccg ttgacgttgc gcggctggct
     5701 gcgctgatcc agtccgttga ccggatcggc ggcgcaggcg gccgacggtc ccttgtcgat
     5761 ggccggactg ttcgcgggca gcgcgtgggt cagcgtctgc ccggtgttgt cggccagcgg
     5821 ccccacaatg gccgccagcc ccaccgcggg caccacgtcg gtggccccgg cggcgaaccc
     5881 ggccagcccg gcattgccgc cggcgccgaa caggttatgg ctgtcggccg tcactgtgcc
     5941 ggccgtccgg ttcacctcgc ggccgacggg ggctgtgttg ccggccagca gcgtccgttg
     6001 cagcgtcagc gacccgccga tcacttgcgc gccgccgcct gcttcgcccg ccgagttgcc
     6061 ggtgacggtc gaattgacca gcgtcgccgt gccgcgcgaa tacagaccac cgccggtagc
     6121 cccggccgag ttgccgctca gcgtgctgtt gaccaccagc aggggcgtcg cgctgtcgac
     6181 ctggatcgcg ccgccgttca tattggccga gttaccgctc agggtgctgc ttttgatcgc
     6241 cagcgagccg ccaccgacga atgcgccgcc gccatactcg gcttcgttgc cggtgatgct
     6301 cacccggtta atcgtgccgg tgacgtccat gaaatagaat ccgccgccgt aggagtaatc
     6361 ccctttgccg ctgttgttgg tgatcgtact gtcggttagc gacaggtcgg agtaattgat
     6421 catcatcccg ctgcctgtca tcgcccggtt gccatcgata gatacattgc tgatggtgac
     6481 tatggcgttg ttgaggtaaa gcccaccgcc cgtgccatag ccatccgcct tattatcgct
     6541 aatggtgctg tcgacgatgg tgatcgagcc gtagccggcg tagacgccgc caccgccgta
     6601 ggatgaattg ttgctgatgg tcgaatcgct gatcgtcagg tcggtgtagg cggcgaagac
     6661 gccgccgccg ttgccgtcgg tcccgttgcc ggtgatggtg cagttgttga gcgtcagctt
     6721 ggcctgatag gcgtagaccg ccccaccctg atagtaggcg tttttgccgt tggtcaaggt
     6781 caggtcattg agcgtcagat tagggacatt cccttcactg tagggcgcgg cggtgaagaa
     6841 gcgcatgtcc tttttgcccg tgcggttaat ggtcgagccg ttgccctcga cagtgattgt
     6901 cgaggtgatg acgggcagcg ccgtgtagcc ataggaataa tcggccatga ttttgctgac
     6961 gctgaacagg ccgccggccg gtaactggat gacgtcggct ccggcggggt cgccgggggc
     7021 gcagtccgtg ttgtcggcca cgccgtcggt ggtgtcgttg gcgttgttga tcgcctcgat
     7081 gagcgagcac ttgccgttat tggcgacgct tacctcgccg ttgacgacgg tgatggtgtt
     7141 gtcggtctcc gcccgcgcgg caaattgggc gcccatgccg gccatggcca gcagcagagc
     7201 cgccccggtc accgtgacgg ccaacttgcg tcgcaattgt cgtcgccaac cgcgcggccg
     7261 ttcggccagc cgcgcataga gccgggcaaa gcgtggtatc accatgtcgc cccttcgcaa
     7321 tagaatactc gccagccaat ggcgtgtctg ctcccggttt gtcgtcgttg attcctgctc
     7381 gttcatgtcc gttcctccca aatgagatag atcggctcct cccgtcgggc attatacaat
     7441 aagcgggcgg aaaattggtg attttgggtg gaaattcgtt gggggcgtga agatgaagta
     7501 cgaaggaaga gcgctcctta ttgatcgccg accggcgacg gccaaccgcc gcaagagcgc
     7561 cacacacaga aaccgggttt ttcgaaaaaa acccggtttc tgtgaagtac gaagtacgaa
     7621 ggaagagcgt gcgtgtctct ttcgtacttc gtacctcgta ctttggaatg acgaatgaca
     7681 agcacgttct gaactcgtca ttcgtcattc gtcactcgtc actaattcaa tggcgcgcac
     7741 atgtagttgg cccgcatgcc gaccagcgcg gccgaatcgc cgatcacgtc atccagcgcc
     7801 gccgacggcg agtagacggt gatggtgatc atgtccgtgc cctcgggcac atccagcgtg
     7861 aaggtcatca ggttcagctg ttcgccgtcg ctggggttgt tgggcttctg cgtttgcgtc
     7921 acgccgcccg cctcaaccgt cacccacacc gggcgggcgt ccttgtcgtt gtcgacgagg
     7981 gccagctcca cgttgaacgt cgtcggcccg ttgggcgggg cgatcgactc ggtgatcacc
     8041 tgcgtcggtg tccagcccag cgccgtgtcc caatagacct cggcttcggc cgcgtcgtag
     8101 gtgttccaga tgttgaccca ggtgttgacc ggatcggcgt aggtcggata gagcaccagc
     8161 gcgcggggga tgtggttctt cgtgccgctc gactgcagat accagcggcc ggtgaccgac
     8221 ttggtgggca gcttggcggc ggggatgtag tcgccgtacc agaagttgcc cgcctgctgg
     8281 gcggccgggc tggtgatcgc
//
LOCUS       DAOMQG010000124         8163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3791_length_8163_cov_10.246053, whole genome shotgun sequence.
ACCESSION   DAOMQG010000124 DAOMQG010000000
VERSION     DAOMQG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3791_length_8163_cov_10.246053"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1892
                     /locus_tag="PLC06_08425"
     CDS             306..1892
                     /locus_tag="PLC06_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRO90508.1"
                     /translation="MGEQHPIDNVDQLNTQRPRFIIAFVAFLFLFVFLFGLPFAAFSD
                     ETLFEPVDGLMTTQVVPVGARRVRAVQVNWSALTPRVDELHLNLFDDVNLTARFSRAD
                     SSITGAYVWVGEVIGEPGSTVTLAVQDGVLSGSVHRFGRAWVVIEYAGGATPDLYTIR
                     EIDPNTPQPTGPDYVVAPQSAADNDTRSSQTATCQEDGSKISLLIAYTAQARDMVGGT
                     QAIIALINRRISEMNTANDVSRVDFDWVLADVEEVDYTESGNIGSDLGNLAGRTDGYM
                     DNIHASRDATKADLVALLISQGSDGRCGLANQLLTAQPNPEIAFGVTALDYPGDYLCD
                     ELTLTHELGHNLGNAHDRFSAGNNGVFPYSYGFQSPQNTFRTLMAYNCPNGGCLRINQ
                     WANPAVWYQNEPTGIDFGMDPDNASDVARSMNETRFMISNYRADCIDLTPTSTATASP
                     TATATPTNTATATLTATPTSTATVASTATNTPTATSTPPGPTVTPTRTRRPTATPEPT
                     ATPTRDTESHTLFAPLVIHR"
     gene            complement(1974..2522)
                     /locus_tag="PLC06_08430"
     CDS             complement(1974..2522)
                     /locus_tag="PLC06_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HRO90509.1"
                     /translation="MNPITFQGDTGQRGAYLLWMRAREQASVTFGRFQGGRPVAISPG
                     WYVYVGSAMGPRGASALAGRLLRHATRTGDKPPHLIRETMGKAFAAAGLGATSLRPPT
                     LKSLHWHVDYLLDQTAIELEHVTVIRTGTRIESALARQLAALPGASTLLKGLGAGDAP
                     GETHLWYLGDGECAPAAFIPAI"
     gene            complement(2548..3486)
                     /locus_tag="PLC06_08435"
     CDS             complement(2548..3486)
                     /locus_tag="PLC06_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010996144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRO90510.1"
                     /translation="MNDLILAFDFGGTKLSAAVINRDALGDERPQWPALRREFTPADA
                     DAAYELAAMLRLARELLAGRSPATVGVSFGGPVDFPRGLVRLSHHVRGWENVPLRDLV
                     AHEFGCPAFVDNDANVAALGEHRFGAGRGVDDLMYITVSTGVGGGWILGGRPWRGHAG
                     MAGEIGHTVVDPAGPLCLCGKRGCVERLASGPYMAADWEEGRGGKGEREKGSGGVTGK
                     DVAVAAAAGDPVAKEILLRGARALGMGIGNAANLVNPQRFILGGGVTKAGAIWWGEVR
                     RAARATALPEVAFDVVPAMLGDDAPLWGAVALTEQL"
     gene            3799..5346
                     /locus_tag="PLC06_08440"
     CDS             3799..5346
                     /locus_tag="PLC06_08440"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRO90511.1"
                     /translation="MAKDMEAYLQDLQMRRASAAGTEKDAATQHGKNRLTARERIDLL
                     FDPDTFTEIDTLVLPRHENYPGGKSSRLGDGVVTGFGLVNGRRVFAASQDAAVMGGSL
                     GEMHANKIVKAMQMALKYGCPFIAINDSGGARIQEGVDSLGGYARIFDANCEASGVIP
                     QLSVIMGPCAGGAVYSPALTDFVFMTNNSYMFITGPDVVRSVMQENVSFEELGGGMMH
                     SAESGVCHFLTDDDRDCLARVRTLLSYLPGNNQDDPPYVKPMDDPERRCPELKEIVPT
                     DPYKAYDVRQVIGSVVDDGVFFEVHQLWAENMVVGFARLNGYVVGIVANQPMVLAGCI
                     DIKASIKATHFIRICDAYNVPIITLQDVPGFLPGKEQEYGGIIRNGSRMIYAYSEATV
                     PKLMVILRKSYGGAYCVMSSKGLRGDLLYAWPNAEIAVMGAEGAVNILFRREVQAAED
                     PEAKRRELVDDYQTKFNNPYVAAARGLIDDVIDPAETRRVLIRSLEVTLSKRERHVPR
                     KHGISPM"
     gene            5365..6849
                     /locus_tag="PLC06_08445"
     CDS             5365..6849
                     /locus_tag="PLC06_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRO90512.1"
                     /translation="MFKKILIANRGEIAVRIIRTCREMGVRTVALYDASDRESLHVRL
                     ADECVQLPDHHAFMDVERVLAIAREQGADAIHPGYGFLAENAMFIHACDAAGIAFIGP
                     PAEVIEAVNTKIGALDKARAAGFPTVTYSRRSFSEDEFDALAADAAAIGYPLVVKSCR
                     GGRGRGERLVNDPERLLEVVRRARAEARAIYGRNELYIEKAILPAHQVGVQIVADRHG
                     DLVHLGDREGSIIHSNQKIVEEAPALCLSPERREALLETAVRLARLFNYQNVGTIEFL
                     VDAAGDFYFTEIKARIQIDHTLAEMTTRIDLVREQIRLAAGERLGYGQADVDIRGCAI
                     MCRLQAEDPSRRYLPSPGHLRRVRLPGGPEVRVDTYLYCDSEVPPFYDPLIAKVTVWA
                     TDRTAAIDRLRRALEDFAIVGAPTNLPLLMTILRAPDFVSGSYATDFLRESPVAKPAT
                     DIELLRRDLAVAAAILHARRHEAFVPEVPEQWATGWHRTSRHIQ"
     gene            6865..7362
                     /locus_tag="PLC06_08450"
     CDS             6865..7362
                     /locus_tag="PLC06_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRO90513.1"
                     /translation="MNTLQVTIDDHIFTVKLTPASRGGETYDVTVDGHNLSVVLPRPK
                     ATAAEMEWMIIDGRPYEIVADTNLQWLNAYDGLHTVEVEDSNQRVARPRSGDGRVKAP
                     IPGLITRVLVEPGAAVQAGQALVILEAMKMENEIRAPFDGVVSSISASGGQSVVRNQV
                     LVEIQ"
     gene            complement(7409..>8163)
                     /locus_tag="PLC06_08455"
     CDS             complement(7409..>8163)
                     /locus_tag="PLC06_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRO90514.1"
                     /translation="RRLRGALANTTLLGLKTNQACLAHILNQPAFLSGETTTSFLVEH
                     GHMSAVSTDKRALSLVAAALARYRTDAGDGPGYWRNNPGTPAPYRFRVGDEAIDVILR
                     PERWLPNRFSATLSSGESVIATIDRFDGVEMALITDGVRHRFILAVRGGDWWVKAGWG
                     ALQITALPLLPAPRRSADMGRSLRAPMPGRVLAVLVEVGQLVEEGQPLMKLEAMKMEH
                     TIRAAAAGVVDAVYYAVGDQVGADETLVRIAG"
BASE COUNT         1563 a         2574 c         2464 g         1562 t
ORIGIN      
        1 atatcccaca agacgactcg acgtcggtaa aatgaccgtc cctattatag caggagttct
       61 cgtcgagact ttagtacctt ttacacaagc ccgttttgat tatgagaggc atcctaatgg
      121 ttgataaata accctttgcg ggttcgaatc cgtggtctaa atttcgtccc gcgccgccgt
      181 ggccccgcgc cgccgtggcc ccgtgagttg tggcgctgcg atatggttat aaggctgata
      241 tcatcatcgg cggcaatccc aggtcggtag cagtattcaa atcggccgta tgggtggagt
      301 ttggaatggg tgaacaacat ccaattgata atgtcgatca attaaatact caacgcccgc
      361 gcttcatcat agcgtttgtg gcttttctgt tcctgtttgt tttcctcttt ggcttgccgt
      421 tcgccgcttt cagcgatgag acgttgttcg agcctgtcga tggcttgatg acgactcagg
      481 tagtgcctgt gggcgctcgg cgagttcggg cggtacaagt caactggagc gcactgaccc
      541 cgcgtgtcga tgagttgcac ctcaacctgt tcgatgacgt caacctcacc gcccggttta
      601 gtcgagccga tagctcgatc accggcgcat atgtgtgggt gggcgaggtg atcggagagc
      661 cgggcagcac cgttacgctt gccgttcagg acggcgttct ctccggcagc gtccatcgct
      721 tcggccgcgc gtgggtcgtg atcgaatacg ccggcggggc aaccccagac ctgtacacga
      781 tcagagagat cgatccgaac acgccccaac cgaccgggcc tgattatgtt gtggctccac
      841 agtccgcagc cgataatgat acccgctctt cgcaaacggc tacctgtcag gaggacggct
      901 cgaaaatcag cctgttgatc gcttacactg cacaggcgcg cgatatggtg ggtggcactc
      961 aggctatcat cgctctcatc aaccgccgca tcagcgagat gaacaccgcc aacgacgtca
     1021 gccgggttga ttttgattgg gtgctggccg atgtcgaaga agtcgactat accgaatcag
     1081 gcaatatcgg cagcgatctt ggaaacctgg cgggaaggac ggatggctac atggacaata
     1141 tccatgcgtc ccgcgatgcg acgaaagccg atctggttgc tttgcttatc tcccaaggga
     1201 gtgatgggag gtgtggcctc gctaatcaac tgttgactgc ccagcccaac cctgaaatcg
     1261 cctttggagt gactgccctt gattaccccg gagattattt gtgcgatgag ctgacattga
     1321 cccatgagtt gggtcacaat ctcggcaatg cccacgaccg atttagcgcc ggcaataatg
     1381 gggtatttcc ctattcttac gggttccaat cgccgcaaaa tacattccgt accttgatgg
     1441 cctacaattg ccctaatgga ggatgccttc gcatcaacca atgggccaat cccgccgttt
     1501 ggtatcagaa cgaaccgacc ggcattgatt ttggcatgga tccggacaac gcttccgatg
     1561 tggctcgttc catgaatgag acgcggttta tgatatccaa ttaccgcgct gattgtattg
     1621 atttgacgcc aacaagcacc gcgactgcct ctcccaccgc aaccgctacg cccacgaaca
     1681 ctgcgaccgc taccctcaca gctaccccca cgagtaccgc gactgtcgcc tccaccgcta
     1741 cgaatacgcc tacagccacc tcgacacctc ccgggccgac ggtcaccccc accaggacgc
     1801 ggcgccccac ggcaacgccg gagccgacgg cgacacctac gagagacacg gaatcacaca
     1861 ccttatttgc accgctggtc attcacagat agaccgggcc ggcacatgcg actgttggat
     1921 ggggctggac ggcaaacaga taacggcggg gggaacgcgc ttcggcgtta tgcttatatc
     1981 gccggtatga aggcggccgg cgcgcactcc ccgtctccca gataccatag atgggtctct
     2041 cccggcgcgt cacctgcccc caggcctttc aggagcgtcg atgcccccgg cagagcggcc
     2101 aactgccggg caagcgcgct ctcgatgcgc gttcccgtcc tgataaccgt cacatgctcc
     2161 aactcgatgg ccgtttggtc cagcaggtaa tccacgtgcc aatggaggct ctttagcgtt
     2221 ggtggccgga gcgatgtggc tcctaatcct gcggccgcaa atgcttttcc catcgtctcg
     2281 cggatgagat ggggcggctt gtcgccggtg cgcgtggcgt gacgcagcag ccgtccggcc
     2341 agcgccgaag cgccccgcgg ccccatggcc gacccgacgt agacgtacca gccgggagag
     2401 atggcgaccg gacggccgcc ctggaagcgg ccgaacgtta ccgacgcctg ttcacgcgct
     2461 cgcatccata acagatatgc gccgcgttgg ccggtatcgc cctggaaggt gattgggttc
     2521 atggtagtcc gacaggcctg ccaagcctta aagttgctcg gtaagcgcca ccgcccccca
     2581 cagcggcgcg tcatcgccca gcatcgccgg aacgacatcg aaggcgactt ccggcaaggc
     2641 cgtcgcgcgg gcagcgcgac gcacttctcc ccaccatatc gcgcctgcct tcgtcacgcc
     2701 gccgccgagg atgaaccgct gcgggttgac cagattggcc gcgttgccga tgcccatgcc
     2761 cagcgcccgc gccccgcgca acaaaatttc cttcgccacc gggtcccccg ccgcggccgc
     2821 cacagccaca tccttcccgg tcactccccc gctccccttc tccctttctc cctttcctcc
     2881 ccgcccctct tcccaatccg ccgccatata cggccccgac gccagccgct ccacgcagcc
     2941 gcgtttgccg cacaggcaga gcgggccggc cggatcgacg accgtgtggc cgatctcgcc
     3001 cgccatgccc gcgtggccgc gccacggccg cccgcccaga atccacccgc cgccgacacc
     3061 ggtgctgacg gtgatgtaca tcaggtcatc gacgccgcga ccggctccga agcgatgctc
     3121 acccagcgcg gcgacgttgg cgtcgttgtc cacgaaggcg gggcagccga attcgtgcgc
     3181 caccaggtcg cgcagcggca cgttctccca gccgcgcacg tggtgggaga gacggacgag
     3241 gccgcgcggg aaatctaccg ggccaccgaa gctgacgccg acggtcgcgg gagagcgtcc
     3301 ggccaacaac tctcgcgcca gccgcagcat tgccgccagc tcataggccg cgtcggcatc
     3361 cgccggggtg aattcgcggc gcaatgccgg ccactgcggc cgctcatcgc caagcgcgtc
     3421 gcgattgatg acggccgcgc tcagtttcgt cccgccgaaa tcaaaagcca ggatcagatc
     3481 attcatgagg tgaatgatag cagactggtg gggggagggc agtggtcagt aagtcagtag
     3541 atcagtgggt cagtagatca gtgggtcagt ggtcggcgat tggcggtcgg ccgtcatcct
     3601 gccacatgac agatcgcgcc aaaacaggtg acggatgaca ctaagaagcg gctgttggat
     3661 atgcaacaat actagcgacc ggcgggagag tatcccacct ctccagcgtt gagcgcgccg
     3721 atcatcccgg tgggggagcc acattacctg ccggctcgat attgcttaaa agtccggccg
     3781 gatatttagg agacacgaat ggctaaggat atggaggcct atctccagga cctgcaaatg
     3841 cgccgcgctt cagccgcggg cacggaaaaa gacgcggcca ctcaacacgg caagaatcgc
     3901 ctgaccgccc gtgaacggat cgatctgtta tttgatcccg acacgtttac cgagatcgac
     3961 acgctggtgt tgccacgcca cgaaaattat cccggcggca aaagttcgcg cctgggtgac
     4021 ggcgttgtga caggctttgg actggtgaac ggccggcggg tgttcgctgc ttctcaggac
     4081 gcggccgtca tgggcggttc gctgggcgaa atgcacgcca acaagatcgt caaggcgatg
     4141 cagatggcgt tgaaatacgg ctgcccgttc atcgccatca acgactccgg cggcgcgcgc
     4201 atccaggaag gggtggacag cctgggcggc tatgcccgta tcttcgacgc caactgcgag
     4261 gcttcgggcg tcatcccgca gctgtcggtc atcatggggc cgtgcgcggg cggcgcggtc
     4321 tattcgcccg ccctgaccga tttcgtcttc atgaccaaca acagttatat gttcattacc
     4381 ggccccgacg tggtgcgctc ggtgatgcag gaaaacgtca gcttcgagga gttgggcggc
     4441 ggcatgatgc acagcgccga gagcggcgtt tgccatttcc tgaccgacga tgatcgcgat
     4501 tgcctggccc gagtccgcac cctgctcagc tatttgcccg gcaataatca ggatgatccg
     4561 ccttatgtta agccgatgga cgatcccgaa cgccgttgcc ctgagctaaa ggagatcgtg
     4621 ccgactgatc cctataaggc ctacgacgtg cgtcaggtca tcggcagcgt tgtcgacgat
     4681 ggcgtgttct tcgaagttca tcagttatgg gccgagaata tggtggtggg cttcgcgcga
     4741 ctgaatggct acgtcgtcgg catcgtcgcc aaccagccga tggtgctggc cgggtgcatc
     4801 gacatcaagg cttccatcaa ggccacccac ttcatccgca tttgcgacgc ctacaacgtg
     4861 cccatcatca cgctccagga cgtgcctggc tttctgcccg gcaaggagca ggagtatggt
     4921 ggcatcatcc gcaatgggtc acgcatgatc tacgcctaca gcgaggccac cgtgccgaag
     4981 ctgatggtga ttttgcgcaa gagctacggc ggcgcctact gcgtcatgag cagcaagggg
     5041 ctgcgtggcg atctgcttta tgcctggccc aatgccgaga tcgccgtgat gggcgcggaa
     5101 ggggcggtga acattctgtt ccggcgcgag gtgcaggcgg ccgaagaccc cgaagccaag
     5161 cggcgcgagc tagtggacga ttaccagacc aagttcaata acccgtatgt ggccgctgct
     5221 cgcggcctca ttgacgacgt gatcgatccg gccgagacgc gccgcgtact cattcgctcg
     5281 cttgaggtga ccctgtccaa gcgcgagcgt cacgtgccgc gcaagcacgg gatttcgccg
     5341 atgtagatca ggagcactac tgaaatgttc aaaaaaatcc tcatcgccaa ccgcggcgag
     5401 atcgcggtgc gaatcatccg cacctgtcgc gagatgggcg tgcgtaccgt ggccctctat
     5461 gacgccagcg atcgcgagtc gctccatgta cgtctggcgg acgaatgcgt ccagctgccc
     5521 gaccatcacg ccttcatgga tgtggagcgg gttctcgcta tcgcccgtga acaaggcgcc
     5581 gacgctatcc atcccggcta tggtttcctg gccgagaatg cgatgtttat tcatgcttgt
     5641 gatgccgccg gtatcgcctt catcggaccg ccggccgagg tgatcgaggc ggtgaatacc
     5701 aagatcggcg cgctggataa ggctcgcgcg gccggattcc cgacggtgac ctattcgcgg
     5761 cgctctttca gcgaggatga gtttgatgct ctggcggccg acgccgcagc aattgggtat
     5821 ccactggtcg tcaagtcgtg tcgcggcggc cgcggtcgtg gggaacgact ggtcaacgac
     5881 ccggagcgat tgctcgaggt cgttcgtcgc gcccgggccg aagccagagc catctacggc
     5941 cgcaacgagc tttatatcga gaaggcgatc ctgcccgccc atcaggttgg cgtgcagatc
     6001 gtggccgacc ggcatgggga tctcgtccat ctgggcgatc gcgaaggttc catcatccac
     6061 agcaaccaga agatcgtcga agaagcgccg gcgctgtgcc tctcgcccga acgacgcgag
     6121 gcgctgctgg aaacggccgt tcgcctggca cggctgttca actatcaaaa tgtcgggacg
     6181 attgaatttc tggtcgatgc cgccggagat ttctacttca ccgagatcaa ggcccgtatc
     6241 cagatcgacc acactctggc ggagatgacg actcgtatcg atctggtccg cgagcagatt
     6301 cgcctggctg cgggggaacg gctgggctat ggacaggctg atgtcgacat tcgcggctgc
     6361 gcgattatgt gtcgcctgca ggccgaagac ccatcgcgcc gctacctgcc cagccccggc
     6421 catctgcggc gggtgcgcct gccgggtggc cccgaggtgc gcgtcgatac ctacctctac
     6481 tgcgactccg aggtgccgcc gttctacgat ccgctcatcg ccaaggtgac ggtatgggcg
     6541 accgacagga cggccgccat cgatcggctg cgccgcgctc tggaagattt cgccatcgtc
     6601 ggcgcgccga cgaacctgcc cttgttgatg acgatccttc gcgcacccga tttcgtctcc
     6661 ggctcgtatg ccaccgactt tctgcgcgag tcacccgtcg ccaaacctgc caccgacatt
     6721 gagttgttgc ggcgggatct ggccgtggcc gcagccattc tccatgcgcg ccgccatgaa
     6781 gcatttgtac cggaagtgcc cgagcagtgg gccacaggat ggcaccgcac cagccgccac
     6841 attcaatagc gccggaggag agatatgaac acgctccagg tcacgattga tgaccatatc
     6901 tttacagtta aactgacacc cgcctcgcgt ggcggagaga cctacgatgt gactgttgat
     6961 ggtcataatc tgtcggtcgt cttgccgcgg ccaaaggcta cggcggccga gatggagtgg
     7021 atgatcatcg acggccgccc ttatgagatc gtcgccgaca cgaatttgca atggctcaat
     7081 gcctatgacg ggctgcacac ggtcgaggtg gaggatagca accagcgcgt ggctcgcccc
     7141 cgcagtggcg acggtcgagt caaggcacct atccccggac tcatcacgcg cgtcctggtt
     7201 gaacccggcg cggccgttca ggccggtcag gcgctggtca tcctcgaagc catgaagatg
     7261 gaaaacgaga tccgcgcgcc gttcgacggt gtggttagct ctatttccgc gtccgggggg
     7321 cagtcggtgg tgcgaaacca ggtgctggtg gagattcagt agctattttg agaagcaggt
     7381 cggcgctcgg gccgaggtca attggcgctc aaccggcgat tctgaccagc gtttcatccg
     7441 cgccgacctg gtcgcccacc gcgtaataga cggcgtccac cacgccggcg gcggcggcgc
     7501 ggatggtatg ctccatcttc atcgcctcca gcttcatcag cggctggcct tcttccacga
     7561 gctgtccaac ctccaccaga acggccagca cacgaccggg catcggcgcg cgcagcgaac
     7621 ggcccatatc ggccgagcgg cggggcgccg gcagcagcgg cagcgccgtg atctgcagcg
     7681 cgccccagcc ggctttcacc caccagtcac cgccccgcac agccaggatg aacctgtgcc
     7741 gcaccccgtc ggtgatcaac gccatctcga cgccgtcgaa gcgatctatc gtggcgatga
     7801 cgctttcgcc cgatgacaga gtcgcgctga agcgattggg cagccaacgc tccggccgca
     7861 agatgacgtc gatggcttcg tcgccgaccc tgaaacgata aggggcgggc gtgccggggt
     7921 tattacgcca gtagcctggt ccgtcgcccg catcggtgcg gtagcgggcc agcgcagcgg
     7981 cgacgagcga cagggcgcgt ttgtcggttg ataccgctga catgtgtcca tgctcaacga
     8041 gaaagcttgt ggtcgtctcc ccgctcagga atgccggctg attcaggata tgagccagac
     8101 aggcctggtt tgtcttcagg cccagaagcg tggtatttgc caacgcgcct cgcaggcgac
     8161 gaa
//
LOCUS       DAOMQG010000125         8139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3809_length_8139_cov_6.703859, whole genome shotgun sequence.
ACCESSION   DAOMQG010000125 DAOMQG010000000
VERSION     DAOMQG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_8139_cov_6.703859"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLC06_08460"
     CDS             complement(<1..258)
                     /locus_tag="PLC06_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90515.1"
                     /translation="MYEVVWQYDADAPFDDPNPASTAEARQMLIDGNRAFARFFTPRR
                     DGSTARRVVRVSPQDVGISATPGEAPPQVPFAAFLSCADARV"
     gene            506..1741
                     /locus_tag="PLC06_08465"
     CDS             506..1741
                     /locus_tag="PLC06_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRO90516.1"
                     /translation="MTPEFERSLSQYAELIVKVGLNLQPGQRLMIRGPIEAAPLMRLV
                     AAHAYDHGGRLVEAAYSDSELQLTRFRHAPRDSFTEHSNWWWKAGLELVEAGGALLSI
                     GGVDPYLLKDVDPELIGIVQRTEGQAALPFSSLISADAINWCVVGYATEGWASRVFPD
                     LSPAEAQERLWQAIFRTVRLDAADPLAAWEAHNANLAARSAWLNGKQYAALHYRGPGT
                     DLTVGLPENHVWIGGVAPTGIGISNTANLPTEEVFTAPHRDRVNGTVRATMPLNVGGN
                     LIEDFSLTFENGRVVDLKAARGEALLRAQVEMDEGASYLGEVALVPNSSPISQSGIIF
                     YNTLYDENASSHLALGRAYRNCIAGCEEASDEEFVARGGNLSANHRDFMIGSAEIDID
                     GITAGGGREPLMRGGEWAE"
     gene            1922..3289
                     /locus_tag="PLC06_08470"
     CDS             1922..3289
                     /locus_tag="PLC06_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90517.1"
                     /translation="MVILKHRRPLLFVIVVAGLVAALFGGRGAAAAPDGAAANAGWAE
                     IGPGSATGGGISRTTKALFPSLAIGPDGKPVVAWYNASSGVFDIYVKQWNGSAWAELG
                     SGSASGGGISHNPDSSLDPSLAIGSDGKPVVAWQDRSNGSSYEIYVRHWNGSAWAELA
                     GSASAGGISKNAGESAEPSLAIGHDGKPVVAWQDNSGGNGEIYVRRWNGSAWVELAGS
                     ASGGGISKNAGDSKNPSLAIASDGNPVVAWEEYSGGNPEIYLRHWNGSAWAELGGSAT
                     GGGISNTGHLGNWSRKPSLAIASGGAPVVAWEHFGGNGFEIYARRWNGSAWAELGGSA
                     TGGGISDNAHDSVEPSLAIGSDGAPVVAWHDQSNGNNYQVYARRWNSAGWVEVGTGSA
                     SGGGISHSNVDAMHPSLAISPNGDPVVAWNDGTNQTNKWDIYVRRYSPAPMPFKALIP
                     AAFQP"
     gene            complement(3399..5201)
                     /locus_tag="PLC06_08475"
     CDS             complement(3399..5201)
                     /locus_tag="PLC06_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HRO90518.1"
                     /translation="MKKQVLIKEIIPAMAIAMILMLAACGNRPTGDTPPAGSGTITGN
                     VIYLDRSGLDPNAVIEVDLFQATPGYPEVVLATQSVNAEGRQVPIPFEIAYDPAQIDT
                     TQTYLVRARILVNGAPAYASQAAVPVITNGAPTQDVEVLVGPVMTGIAGGTLTGNVIY
                     LERIAMAPDAVITVELQDASAGTVAVIAAQDINAEGRQVPIPFELPYDATRIDPAGTY
                     LLSARIAENGQTTFASQTGVPVLTNGAPTSNVEVLVSPAAPTAGGVLRGSVTAPRQLE
                     PFDPAAVLQVELREPMKADAPVAAIVEIPLAGLSFPVSFELPYDPASIAADRAYVVGA
                     RVLAGNRLFYNSLAPVPVLTNGAPASEIAVPVAAVPNPAGGILRYTITSDTPMTWAAD
                     STAWLNVEIREPMLADAPAAAFGYVPLAGLSFPISFELGYDPAQFDPNRAYIFDARII
                     DNNLLTFSSSEGTPALTQGAPVTDVTLNLTQAAVVSPTPNDAPPSGAITGVVTTDAPT
                     PLDPTAALYVDFREAGTTGDPIVTISKTLEGAQFPIPFEVPLAAATIDPTRNYVVGAR
                     ILLGDQVLYASQVGVPVVTNGAPTTDVVVNIPPQ"
     gene            5501..6640
                     /locus_tag="PLC06_08480"
     CDS             5501..6640
                     /locus_tag="PLC06_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90519.1"
                     /translation="MNKFVFAFLGLLGGLSLCVADSRAAAPANPVLSIPVPIWSEESI
                     GPGGTLNDVAVDSAGNPHVLYVDMAAEELRYARRVDGAWVVTPLGPFPVGSPPPDMVL
                     DLAIDPTNDEPVVAYSNIERNSLYVGRPNEGIWWFERLDDIGQLLSLRVDPAGDAHLV
                     YVRGSRIAYTYQVGGVWQREMIGEPVDAMWNLSMDLDSAGFPHVAWSGSSGSFHAQRL
                     GPNAWDITTLPASELRQVIGLAVDPADQPHFLVMKVELGHGWPPYSRITLLLAEHTGG
                     SWEFTPLWQAYDWGVDTRLVAGADGSLHVAYYDSDARPHYDVVDPDGRLSHDQPFDRG
                     QGQMRLAPGTDGRPFISHFDGHTLFLSRQGIALHDRLIFGPAALR"
     gene            6804..7475
                     /locus_tag="PLC06_08485"
     CDS             6804..7475
                     /locus_tag="PLC06_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRO90520.1"
                     /translation="MGLNHRRMNEPVNEYYTLGEEIAHSVTHGIGAALSLAALVTLLV
                     LAIIHGTQWHILSFAVYGVSLFAVYLASTLYHGVQKPRLRPILRKVDHACIYLLIAGT
                     YTPFVLISMRSSLGLTLLTAVWAMAVFGIVYKIFFIDHFAVLTTLIYVAMGWMSMFAW
                     REMVANIPQTGLVLLVAGGGLYTVGVIFYAMTKIRYTHAVWHLFILGASACHFVAVLT
                     LLNAA"
     gene            complement(7479..>8139)
                     /locus_tag="PLC06_08490"
     CDS             complement(7479..>8139)
                     /locus_tag="PLC06_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRO90521.1"
                     /translation="ILGVGGQQLVVIPDYEGASSYILARLARELSPSLTYHSLSHTRD
                     DVLPAAVRLGKAVHLSDENLLLLKTAAVFHDCGFLVSYDNHEQYSINLAREKLPEFGY
                     SPEQIDTIAETIAATRLPQRPRNLLQQLMCDADLDTLGREDFPELNRRLQEEHQRILK
                     SPPNPQVWLANQVRFLEDHSFFTRAAHEFRDAGKAQNLARIRSRLNSFNGPVAGALGN
                     V"
BASE COUNT         1439 a         2538 c         2640 g         1522 t
ORIGIN      
        1 cacgcgggcg tcggcgcaac tgagaaacgc ggcgaacggc acttgtggtg gcgcttcgcc
       61 cggcgtggcg ctgatgccga catcctgcgg cgagacgcgc accacccggc gcgccgtcga
      121 gccgtcgcgc cgcggcgtga agaagcgcgc gaaggcgcga ttgccgtcga tgagcatctg
      181 tcgcgcctcg gccgtgcttg ccgggttggg gtcatcgaac ggggcgtcgg cgtcgtattg
      241 ccagaccact tcgtacatgg gacatcctcg ccgcgcgggg tcgtgatcgc gcggacaggg
      301 atagcataat cagtggccgg ccaacgcgca aggttgcgcc acagcaaaga ttctttagaa
      361 atcgggtttt ttcctgaaaa cccggtttct aaaaaagact cactccgtag aaaccgggtt
      421 tctcggaaga aactcggttt ctacacaatt ttggggtaat ctggtgccat cgaatcaatc
      481 atccctctca aggagtttcc ccctaatgac cccggaattt gaacgttcat tgagccaata
      541 tgccgagctg atcgtcaagg tcggcctgaa tttgcagccc ggtcagcgat tgatgattcg
      601 cgggccgatc gaggccgccc cgctgatgcg gctggtggcg gctcatgcct acgaccacgg
      661 cggccgcctt gtcgaagcgg cgtacagcga ctccgaactg cagctaacgc gcttccgcca
      721 cgcgccgcgc gactccttca ccgagcattc caactggtgg tggaaggcgg gcctcgaact
      781 ggtcgaagcc ggcggcgcgc tcttgtccat cggcggcgtc gatccctatc tgctgaagga
      841 tgtcgaccct gaactgatcg gcatcgtgca gcgcaccgag gggcaggcgg ctctgccgtt
      901 cagcagtctc atctcggccg acgccatcaa ctggtgcgtc gtcggctatg ccacggaggg
      961 ctgggcgagc cgcgtgttcc ccgatttgtc gccggcggaa gcccaggagc ggctgtggca
     1021 ggccatcttc cgcaccgtgc gcctcgacgc ggccgacccg ttggccgcct gggaggctca
     1081 taacgccaac ctcgccgcgc ggtcagcctg gctgaacggc aagcaatacg ccgcgctcca
     1141 ttatcgcgga ccgggcaccg acctgaccgt aggcttgccg gaaaaccatg tgtggatcgg
     1201 cggggttgcc cccaccggca ttgggatctc caacacggcc aatttgccta cggaagaggt
     1261 gttcaccgcg ccccatcgcg atcgggtcaa cggcaccgtg cgggccacca tgcccctcaa
     1321 cgtgggcggc aatctcattg aggatttcag cctgaccttc gagaacggcc gcgtggtcga
     1381 tctgaaggcg gcccgcggcg aggcgttgct gcgggcgcag gtggagatgg acgaaggggc
     1441 gtcctatctg ggcgaagtcg ccctcgtgcc caacagctcg cccatctcgc aatcgggcat
     1501 catcttctat aacacgcttt acgacgagaa tgcctcatcc cacctggcgc tcggccgcgc
     1561 ttatcgcaac tgcattgccg gctgtgaaga ggcgagcgac gaggagttcg tcgcgcgtgg
     1621 cggcaatctc agcgccaacc accgcgactt catgattggc tcggccgaga tcgatatcga
     1681 cggcatcacc gccggcggcg ggcgcgaacc gctcatgcgg ggcggggaat gggcagaata
     1741 acaaatggat taacagggta ttgacaacgg gattcccgcg atctaggatg aatatcgatt
     1801 cttaatcgca gaccaccagg tgattaaggc gctatccacc tctcccccga tggcctccaa
     1861 acgttattcc tgactgaccg ctccatcgcg agcggaaagt aacatatggg agaacagggt
     1921 catggtaatc ctcaagcatc gtcgtccctt gttgttcgtg atcgtggtcg ccgggctggt
     1981 ggccgcattg ttcggaggcc ggggggccgc cgccgcgccg gatggagcgg cggccaacgc
     2041 cggctgggcg gaaataggtc caggctcggc caccggcggc ggcatcagcc gcactaccaa
     2101 ggcccttttc ccgtcgttgg ctatcggtcc cgacggcaag ccggttgtcg cctggtacaa
     2161 cgcctcttcc ggcgtcttcg acatctacgt caaacagtgg aacggctcag cctgggcgga
     2221 actggggagc ggctcagcct ccggcggcgg catcagccac aaccccgaca gctcgctgga
     2281 tccttctctg gctatcggct ccgacggcaa gccggtcgtc gcctggcagg accgcagcaa
     2341 cggcagcagc tacgagatct acgtccggca ctggaacggc tcagcctggg ccgagctggc
     2401 cggctcggcc tccgccggcg gcatcagcaa gaacgccggc gaatcggcgg aaccttccct
     2461 ggccatcggt catgacggca agccggtcgt cgcctggcag gacaatagcg gcggcaatgg
     2521 tgagatctat gtccggcgct ggaacggatc cgcctgggtc gagctggccg gttcggcctc
     2581 cggcggcggc atcagcaaga acgccggcga ctccaaaaac ccctccctgg caatcgcctc
     2641 cgacggcaac cccgttgtcg cctgggaaga gtacagcggc ggcaatccgg agatctactt
     2701 gcgccattgg aacggctcag cctgggccga actgggcggc tcagccaccg gcggcggcat
     2761 cagcaatacc ggccatctgg gcaattggtc ccggaagccc tctttggcca tcgcctccgg
     2821 cggcgcgccc gtcgtcgcct gggaacattt tgggggcaac ggctttgaga tctacgcccg
     2881 acgctggaac ggatcggcct gggctgagtt gggcggctcg gccaccggcg gcggcatcag
     2941 cgacaacgcc cacgactccg ttgagccttc cctggccatc ggctccgacg gcgcgccggt
     3001 cgtcgcctgg catgaccaga gcaatggcaa caactaccag gtctacgccc gacgctggaa
     3061 cagcgccggc tgggtcgagg tgggcaccgg atcggcctcc ggcggcggca tcagccacag
     3121 caacgtcgac gccatgcacc cctccctggc catcagcccc aacggcgatc ccgtcgtcgc
     3181 ctggaacgac ggcaccaatc agacgaataa atgggacatc tacgtccggc gctattcgcc
     3241 cgcgcccatg cctttcaagg cgctcatccc ggcggcgttc cagccgtaga gcggttcaca
     3301 gataaagaga ggccgcccgg cgacaaaccg gacagcctct ctttttaatc tccacgcgac
     3361 ggacgggtct tcctgacccg tatcccgtat ttcgtgcctt actgtggcgg tatgttcacc
     3421 accacatcgg tcgtcggcgc gccgttggtg acgacgggga cgccgacttg cgacgcgtag
     3481 agcacctgat cgcccagcag gatgcgcgcc cccacgacgt agttccgcgt cgggtcgatg
     3541 gtcgctgcgg ccagcggcac ctcgaacggg atggggaact gcgccccttc cagcgtcttc
     3601 gagatggtga cgatgggatc gcctgtcgtg cccgcctcgc ggaaatccac gtagagcgcc
     3661 gcggtggggt cgagcggggt cggagcgtcg gtcgtcacca cgccggtgat cgcccccgat
     3721 ggaggcgcgt cattgggcgt tgggctgacg acggccgcct gcgtcaggtt caacgtcacg
     3781 tcggtgaccg gtgcgccctg cgtcagggcc ggcgtgccct ccgatgacga gaaagtgagc
     3841 aggttgttgt cgatgatgcg ggcgtcgaag atataggccc ggttcgggtc aaattgggcc
     3901 ggatcgtagc ccagctcaaa agagatcggg aaggaaagcc cggccagcgg cacatagccg
     3961 aaagccgcgg ccggggcgtc ggccagcatc ggctcgcgaa tctccacgtt gagccaggcg
     4021 gtggaatcgg ccgcccaggt catcggggta tcactggtga tcgtgtagcg caggatgccg
     4081 cccgccggat tgggcacggc ggccaccggc acggcgatct ccgacgcggg cgcgccgttg
     4141 gtcaataccg gcacgggggc cagcgagtta tagaacagcc tgttgcccgc cagaacccgc
     4201 gcccccacca cgtaggcccg atccgcggcg attgacgccg ggtcataggg cagctcaaag
     4261 gaaacgggaa aagacagccc ggcgagcgga atctcgacga tggccgcaac gggggcgtca
     4321 gccttcatcg gctcgcgcaa ctccacttgc aggacagccg ccgggtcgaa cggttcaagc
     4381 tgacgcggag ccgtcaccga gccgcgcagg acgccgccgg cggtgggggc cgcgggagag
     4441 accaggacct cgacgttgct ggtcggcgcg ccgttggtca gcacgggtac gccggtctgc
     4501 gaggcgaaag tcgtctgccc gttctcggcg attcgcgcgc tcagcaggta ggtgccggcg
     4561 gggtcgatcc gcgtggcgtc atagggcagc tcgaacggga tgggcacctg tcgcccttcg
     4621 gcgttgatgt cctgggcggc gatgaccgcg acggtgcccg ctgaggcgtc ctgcagctcc
     4681 accgtgatga cggcgtcggg ggccatggcg atgcgttcca gatagatgac attgccggtc
     4741 agcgtgccgc cggcgatgcc ggtcatcacc ggcccgacga gaacctcgac gtcctgcgtc
     4801 ggcgcaccgt tggtgatcac cggcacggcc gcctgcgagg cataggccgg cgcgccgttg
     4861 accagaatgc gcgcccgaac cagataggtc tgtgtggtgt cgatttgggc cgggtcatag
     4921 gcgatctcga acgggatggg cacctgccgc ccttcggcgt tcactgactg ggtagccagc
     4981 acgacttcgg gatagccggg cgtggcctgg aacagatcga cctcgatgac cgcgttcggg
     5041 tccagccccg agcgatcaag gtagatgacg ttgccggtga tggtgccgct gcccgcgggg
     5101 ggtgtgtcgc cggtcggccg attgccgcaa gcggccagca tgaggatcat ggctatcgcc
     5161 atagccggga taatttcttt aatgagcact tgtttcttca cttcttcttt ctccttacgg
     5221 tggggggacg agtgcttccg tggatggtaa gttatctata gtcacccggc ccggcccaag
     5281 cataatcgcc cccgattaga gatgcaacgt tcatgacata aatattaaga tattggggca
     5341 acatcaggca aattacgaaa aagttttttc acttcaattg tagccagacc tgacaggtgg
     5401 gcgattaagg cctcagtcag tcaagtgatt tttcgcccac ctgtcaggtc tgtatacctc
     5461 acctattgtg atcggtgtgg gatagattta tactgtggcc atgaacaagt tcgtctttgc
     5521 ctttctgggg ctgttgggcg gactgagcct ctgcgtcgcc gattcccgtg cggccgcgcc
     5581 cgccaacccc gtcttatcca tccctgtgcc gatctggtcg gaagagtcga ttggccccgg
     5641 cggcactctg aacgatgtcg ccgtcgatag tgccggaaac ccccatgtgt tgtatgtcga
     5701 tatggccgcc gaggagctgc gctatgcgcg gcgcgtggat ggcgcgtggg tggtgacgcc
     5761 gctggggccg ttccccgtcg gcagcccgcc gcccgacatg gtgctcgatc tggcgattga
     5821 cccgacgaac gacgagccgg tggtcgccta cagcaacatc gagcgaaaca gcctctatgt
     5881 cggccggcct aatgagggca tatggtggtt cgagcgcctg gatgacatcg gccaactgct
     5941 gtcgctgcgc gttgatccgg cgggagacgc gcacctggtc tacgtccggg gttcccgcat
     6001 cgcctacacc tatcaggtcg gtggcgtctg gcagcgggag atgattggcg agccggtcga
     6061 cgcgatgtgg aatctgtcca tggatctgga cagcgcgggg ttcccccacg tggcctggtc
     6121 gggatcatca ggctccttcc atgcgcaacg ccttgggccg aatgcatggg atataacgac
     6181 gctgccggcc tctgagctga ggcaagtcat cggcctggcc gtcgatccgg ccgatcagcc
     6241 tcacttcctc gttatgaaag tggagcttgg gcatgggtgg ccgccttact cgcgcatcac
     6301 gcttcttctg gcggaacata cgggcggttc gtgggaattt acgcccctct ggcaggcgta
     6361 tgactggggc gtcgataccc gattggtggc cggggcggat ggctcgctcc acgtggccta
     6421 ctacgactcc gatgcgcggc cgcattacga cgtcgtcgat cccgacgggc ggctatccca
     6481 cgatcagccg ttcgatcgcg ggcaggggca aatgaggctc gcgccgggca cggacggccg
     6541 ccccttcatc tcccatttcg acgggcacac gctgtttctg tcccggcaag ggatcgcgct
     6601 tcatgaccgg ctgatttttg ggcctgctgc gctccgctga gccggtgata atcacgtatc
     6661 gctttcgggc gggattttat attaacaaat taatcacgtg attgatttgt caacgcccaa
     6721 aaaggtgagg attcgcctgt ggacgtgacc gaacctgccc gattgctcta tactcgcgtg
     6781 atgattatcg ggagaaaaac ggcatgggac tgaaccaccg gcgaatgaat gaaccggtca
     6841 acgaatacta cacgctgggc gaggagatcg cccacagcgt gacgcacggc atcggggcgg
     6901 cgctgagcct ggccgcgctg gtgacgctgc tcgtcctggc catcatccac ggcacccaat
     6961 ggcacatcct cagctttgct gtctatggcg tgagcctgtt cgccgtctat ctggcgtcca
     7021 cactctacca tggcgtgcag aagccccggc tgcggccgat cctgcgcaag gtcgaccacg
     7081 cctgcatcta tctgctcatc gccggaacct acacgccctt cgtgctcatc agcatgcgca
     7141 gctcgctggg cctcacgctg ctgacggccg tgtgggcgat ggccgtcttc ggcatcgtct
     7201 ataaaatctt cttcatcgat cacttcgccg tgctgacgac gctgatctat gtggccatgg
     7261 gctggatgag catgttcgcc tggcgcgaga tggtggccaa tatcccccag acggggctgg
     7321 tgctactggt ggccggcggc ggtctctata ccgtgggagt catcttctac gccatgacca
     7381 aaatacgcta cacccacgcg gtgtggcatc tgttcattct gggggccagc gcctgccatt
     7441 tcgtggccgt gctgacgctg ctcaacgcgg cgtagccgtc acacattgcc cagcgcgccg
     7501 gcgacggggc cattgaacga attgaggcgg gagcggatgc gagccaggtt ctgcgccttg
     7561 ccggcgtccc gaaactcatg cgccgcgcgg gtgaagaagg agtggtcttc caggaaccgg
     7621 acctggttgg ccagccagac ttgcgggttg gggggcgatt tcaggattcg ctggtgttct
     7681 tcctgcagcc ggcggttcag ctcggggaaa tcctcgcggc cgagcgtatc cagatcggcg
     7741 tcacacatca gctgttgcag caggttacgc ggccgttggg gcagccgggt ggcggcgatt
     7801 gtctcggcga tggtgtcgat ctgttcgggc gagtagccga attcaggcag cttctcgcgg
     7861 gcaaggttga tgctgtactg ttcgtgattg tcgtaggaga ccagaaaacc gcagtcatgg
     7921 aagaccgcgg ccgtcttcag cagcagcagg ttttcatcgc tcaggtgaac ggctttcccc
     7981 agacgcacgg cggcgggcag cacgtcgtct cgcgtgtggg acagcgaatg ataggttaat
     8041 gatggcgaga gttcccgagc aagccgcgcc agtatgtagc tgcttgcacc ctcgtaatcc
     8101 ggtattacca caagttgttg accgccaact cccaagatt
//
LOCUS       DAOMQG010000126         8115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3824_length_8115_cov_8.739330, whole genome shotgun sequence.
ACCESSION   DAOMQG010000126 DAOMQG010000000
VERSION     DAOMQG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_8115_cov_8.739330"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLC06_08495"
     CDS             <1..811
                     /locus_tag="PLC06_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90522.1"
                     /translation="IGRVVWSRADFNDAFALAISSGLTTAILLIANIAWEPRTIPVPY
                     LGHRLLPSGLILVSGVLAFVGFALMRYRMRIITGLSEHWITMRGGAATAGERVLIVGA
                     GEVGNLASYLLRKAELGQVYSIVGLVDDAPRKQGTRISGIRVLGRTDEIPELVSLHDV
                     GLIIYAIEKIRRPQRERILGLCRSTGVQTIILPDVLETFRHQLGAETEAPRVVESTEP
                     ETRETPSSTYRLENWLVEVDRLMQAEAWDAAHEKVVAMQQQVVLQKQASGS"
     gene            910..1902
                     /locus_tag="PLC06_08500"
     CDS             910..1902
                     /locus_tag="PLC06_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO90523.1"
                     /translation="MSEWTGKRVLVTGAGGFIGSQLTESLVRSGAQVRAFVRYNSRGD
                     AGLLRQLPKEITAELEIVAGDLRDTAAVDGAVAGVDVVYHLGAIISIPYSYKHPMETA
                     ETNFMGTLNVLLACRAHNVARLVHTSTSEVYGTAQFTPMDENHPLQGQSPYSASKIGA
                     DKLVESFYRSYSLPAVTIRPFNTYGPRQSARAVIPTIITQALTGDCIRLGNLDARRDF
                     TYVRDTVNGFLLAADASGVLGQEINLGTGADISIGDLTAEIVRQVGRPVEIVTESERM
                     RPEKSEVRRLLSDNGLARRVLGWEPAYALEAGLRETIAWIGDHLDFYHVGRYEI"
     gene            1934..2701
                     /locus_tag="PLC06_08505"
     CDS             1934..2701
                     /locus_tag="PLC06_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRO90524.1"
                     /translation="MKAVILAGGKGTRLRPYTTILPKPLMPIGDMPILEILLRQMKRA
                     GVEDVVLTVGHLAEMLHLFFQDGQKLGLNISYSHEDVPLGTAGPLSLVDGLDDTFLVA
                     NGDVLTTLPLANLLAHHRLSGAAATIAMHRRQVYIDLGVIQQDEGGRVTGYIEKPTYD
                     FMVSMGIYVFEPRVLDYIPRNAYLDFPDLVKRLIAAGEIVTGFPFDGYWQDLGRADDY
                     ERAIEDFDRMKAEFLPPEAPIIHSINGAGKHAQLYQG"
     gene            2706..3908
                     /locus_tag="PLC06_08510"
     CDS             2706..3908
                     /locus_tag="PLC06_08510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRO90525.1"
                     /translation="MEQSKPVWRIPLADVDLGPEEEAAVLEVLRRGWLSMGEVTAEFE
                     AEFAALTGARHALAVTNCTAALHLAGLALGWEPGDEVIVPSLTFVATANAVRYTGATP
                     IFADIISESDFSLSAEDVAERITPRTKAIIVVHYAGHAADMPAIMALAERHGLAVVED
                     VAHAPGADMDGRAPGTWGDIGCFSFFANKNMTTGEGGMVTTDDDELAARLRLLRSHGM
                     TSLTWDRHKGHAWSYDVVAPGYNYRIDEVRAAIGRVQLAKVTAANQRRRALDALYREL
                     LPAVVPELGVPYATTRGTPACHLRPVLLPEGADRRRFMDEMKARGIQTSIHYPPIHRF
                     SYYRNMAGGAALPLTELVGDREVTLPLYPALTTADVESVVAAVAEALDAALDGAGRSL
                     VEMTALPA"
     gene            4006..5934
                     /locus_tag="PLC06_08515"
     CDS             4006..5934
                     /locus_tag="PLC06_08515"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRO90526.1"
                     /translation="MELKLIGQMLRRWSWLLVIGILVGGGIGYAISIYQTPEYLARTK
                     ILVMEPLGDNASNVNNLNDKELAETYMQLLTTQPILESVSQKVGVKVRDTQIKATRVR
                     DTGLLEVSVRDTDAQRAALTANVLVEVLIEYNEALQKSRFSSSEQSLELQVSEIKSEL
                     GRLESNVEQKSEQTLEEQRQTVEQKKTELEQQIFTLQGEIARLQQDIDDLTPEALPNQ
                     LPAPLTLEQRNQLNEKRTQLAQKEFQLDLAKESYSRLAAPTGTAAQNVQDPTNLQNLE
                     QANLELYRQLYSTLLSNYEAVRLARLQNTPNVVQVEKAMPPTIPIQAGPLRNIILGAV
                     IGLLLTGTVAFILEYMDDTLKSPQDIANVLGLPVVGYVVNESGMDKTDGTPYVSANPR
                     SPMAETFRTLRTNLEFASVDKSLKTILITSPGASEGKTTVATNLAAVMAQANKRVILL
                     EGDLRRPRVHRALGMSNQIGLSDVFRGQMDIRDVARYSKVKDLAVITSGSLPPNPAEL
                     LGSARMTQILARLVDSASVVIIDSPPFVVADSTVLASKVDGVLLVIQPGKTHAEAAKT
                     MLAQLDRAGAHVVGVVLNRVSRKTSSYYGYYRYESDPIYNGEDASSEDSARRKGLASL
                     FSGNGKNMNGKLEEKTAS"
     gene            6108..7544
                     /locus_tag="PLC06_08520"
     CDS             6108..7544
                     /locus_tag="PLC06_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90527.1"
                     /translation="MQSSAPYKSIPSSRQSAADASEIPDRCPFLGTATDPAMAHEFPS
                     DANRCYTTRLGVPVSTIHQENYCLSSRFESCPVYRERIKRAGGETIVPPAVIATIAAA
                     SPLVWHDEAHDEAAAADPAPVESAMNGAASTPVDEPVDTAAPLLFPWEMQTGAPHPDF
                     QNDPVAKPYRRQSRSISLRPVLIGLLLLALIPLVWWLWTTVRPGASNEVSGAPGAIVT
                     LPTVVGAGNGGEAGGDRGGTSDLPPAGGESGAIAGGAADEPTMEPTAEPTATDLEKIA
                     ATATALFANATPVTECVAPSWWVAYLVEEGDTIEALAATRGIKPEALIVANCLAGPDL
                     EPGLTLLLPPVGVIASLPEASPTPTATATRRPQVPGLPTRRPIFLPTPTFPVIIILTP
                     QVPVIEPTDPPDEEPPVRATRTPSVSPTATAPNPFPQATLTPPGFITSTPPGFITSTP
                     PGFITSTPPSPGGTIPVTPTMTPPAPGR"
     gene            7623..>8115
                     /locus_tag="PLC06_08525"
     CDS             7623..>8115
                     /locus_tag="PLC06_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90528.1"
                     /translation="MNSTTIGHHQAQNIDINLRRLDAAVLAMTAPFLLAPAYFSPALS
                     GIALSLLSFPYIIRLAFGKPLSRPTIVNLPVGLLAFVFMPLALLMSPATWGTSWERVL
                     TLAWSIALFFALVSWPGSGSKADLRTRFSRPTLIYLGLGTLVALLGLLAMRSVDKLFF
                     LPQT"
BASE COUNT         1509 a         2376 c         2627 g         1603 t
ORIGIN      
        1 cattggccgc gtggtctggt cgcgagccga tttcaatgat gcctttgctc tggcgatctc
       61 gtcggggctg acgacggcga tcttgctgat agccaacata gcctgggaac cgcgaaccat
      121 tccggtgcct tatcttggtc atcgcttgct gccgtcgggg ctgatcctgg tcagcggtgt
      181 gctggctttc gtggggttcg ccctgatgcg ctatcggatg cgcattatca ccggcctttc
      241 ggaacactgg atcacgatgc gcggcggagc ggccacggcc ggcgagcgcg ttctcatcgt
      301 cggcgcgggc gaggtcggca atctggccag ctatctgctg cgcaaggcgg agttgggtca
      361 ggtgtattcc atcgtgggcc tggtcgatga cgcgccccgc aagcagggca cgcgcatcag
      421 tggtatccgc gtgttgggcc gcacagatga aatccctgaa ctcgtgagcc ttcacgatgt
      481 gggtctgatc atctatgcca tcgagaagat ccgccggccg cagcgcgagc gcattctggg
      541 gttgtgccgc tccaccggcg tgcagacgat catcctgccg gatgttctgg agacattccg
      601 ccatcagttg ggggcggaaa ctgaagcgcc tcgcgtcgtc gaatcgactg agccggagac
      661 aagggaaacc ccgtcaagca cttatcgcct ggagaattgg ctcgtggaag tcgatcgttt
      721 gatgcaggcc gaggcctggg atgcggcaca cgagaaggtt gtggccatgc agcagcaggt
      781 cgtattgcag aaacaggcca gcggttcctg attccgtcca agacagggac ggatgatcgc
      841 ctccgagcgc gcgccggctg ccaccggcgt cgtcggaact gaataaatat taggagtata
      901 tcaaccgaga tgagtgaatg gaccggcaag cgcgttctgg tgacaggcgc gggtggcttc
      961 atcggcagcc agctaaccga atcgctggtg cgcagcggcg ctcaggtgcg cgcctttgtg
     1021 cgctacaact cgcgtggcga cgcgggtttg ctgcggcaat tgccgaaaga gatcactgct
     1081 gagctggaga tcgtggccgg cgatttacgt gacacggccg cggttgatgg cgcggtggcc
     1141 ggcgtcgatg ttgtctatca tcttggggcg attatctcca ttccctattc ctacaagcac
     1201 ccgatggaga cggctgagac gaacttcatg ggcacactga atgtgttgct ggcctgccgc
     1261 gcccacaatg tcgcgcgatt ggtgcacacg tctaccagtg aggtctacgg cacggcccag
     1321 tttacgccga tggatgaaaa tcatccgctg caggggcagt cgccttattc ggccagcaaa
     1381 atcggggccg acaagctggt ggagagcttt tatcgctcct atagcctgcc ggccgtcacg
     1441 attcggccgt ttaataccta tggcccgcgc caatcggctc gcgcggtcat cccgacgatc
     1501 atcactcagg cgttgacggg cgattgcatc cgtctgggca atctggacgc gcggcgcgat
     1561 ttcacctatg tccgcgacac cgtcaatggc tttctgctgg ccgccgacgc gtcgggggtg
     1621 ctgggtcagg agatcaatct gggcacaggc gcggatatca gcatcggcga cctgacggcc
     1681 gagatcgttc gccaggtcgg ccgcccggtc gagatcgtca ccgagtcgga gcggatgcgc
     1741 cccgaaaagt ccgaggtgcg gcggttgtta tccgataatg ggctggcccg gcgcgttctg
     1801 ggttgggagc cggcttatgc tttggaagcc ggcttgcggg agacgatcgc ctggattggc
     1861 gaccatctcg atttttatca tgttgggcgg tacgagattt agtccggccg aagcgcaaca
     1921 aggaaaggga acgatgaaag cagttattct agccggcggc aagggcacac gattgaggcc
     1981 ttataccacg atattaccga aaccgttgat gcccatcggc gacatgccta ttctcgaaat
     2041 tctgctgcgg cagatgaagc gagccggtgt ggaagacgtc gtgctgacgg tgggtcacct
     2101 ggccgagatg ctgcatcttt tcttccagga cggccaaaaa ctggggctga acatcagcta
     2161 tagccatgaa gatgtcccgt tgggcacagc ggggccgctg tcgctggttg acgggctgga
     2221 cgatacgttt ctggtggcca atggcgacgt gctgacgacg ctgccgctgg ccaatctgct
     2281 ggcccatcat cgcctgagcg gcgcggcggc gaccatcgcc atgcaccggc ggcaggtcta
     2341 catcgacctg ggcgtgatcc agcaggacga gggcggccgc gtcaccggct atatcgaaaa
     2401 accgacctat gatttcatgg tcagcatggg gatctacgtc ttcgagccgc gtgtcctgga
     2461 ttacattccg cgcaacgcct atctcgattt ccccgacctg gtcaagcggc tcatcgccgc
     2521 cggggagatc gtcaccggct ttccgtttga cggctactgg caagacctgg gccgggcgga
     2581 tgattatgag cgggctatcg aggatttcga tcggatgaag gcggagtttt tgccgcccga
     2641 agcgccgatt attcattcca ttaacggggc gggcaagcat gcccaactct atcagggcta
     2701 agatcatgga acagtcaaaa cccgtgtggc gcattccttt ggccgacgtt gacctggggc
     2761 cggaagaaga agcggccgtc ctggaggtat tgcggcgcgg ctggctgtcg atgggcgagg
     2821 tgacggccga gtttgaggcc gagttcgccg cgttgaccgg cgcgcgccac gcgctggcgg
     2881 tcacgaattg cacggcggcg ctccatctgg ccgggttggc cctgggttgg gaaccgggcg
     2941 atgaggtgat cgtcccgtcg ctgacgtttg tggccacggc caatgcagtg cgctacaccg
     3001 gcgcgacgcc gatcttcgcc gacatcatca gcgagagtga tttcagcctg tcggccgagg
     3061 atgtcgccga gcgcatcacg ccgcggacga aggcgatcat cgtcgtccac tacgccggac
     3121 acgcggccga catgccggcc attatggcgc tggccgagcg ccatgggctg gccgtcgttg
     3181 aggatgtggc ccacgcaccg ggggcggata tggatggtcg cgcaccgggc acgtggggcg
     3241 acatcggctg cttcagcttt ttcgccaaca agaatatgac caccggcgag ggcggcatgg
     3301 tgacgaccga cgacgatgaa ctggctgccc ggctgcgtct gttgcgctct catggcatga
     3361 ccagcctgac ctgggatcgg cacaagggcc atgcctggag ctacgacgtc gtcgcgccgg
     3421 gctataacta tcgcatcgat gaggttcgcg ccgccatcgg ccgggtgcag ctggccaagg
     3481 tgacggccgc caatcagcgc cgccgcgctc tcgatgcgct ctatcgcgaa ttattgccgg
     3541 ctgtcgtgcc ggagttggga gtcccttatg ccacgacgcg cgggacgccc gcctgccatc
     3601 tgcggccggt gctgctgccc gaaggagccg accgccggcg ctttatggac gaaatgaagg
     3661 cgcgcggcat tcagaccagc atccactacc cgccgatcca ccgcttcagc tattaccgga
     3721 acatggcggg cggagcggcg ttgcccttga cggagcttgt gggcgatcgt gaagtgacgt
     3781 tgccgctcta tccggcattg acaaccgccg acgtggagag cgtggtggcg gctgtggccg
     3841 aggcgctcga cgcggcattg gacggagcag ggcgatcgtt agtcgaaatg acagctttac
     3901 cggcttaaat agattggaac ttgggcgttt gtttccttcg ttattggaat tgattgcggg
     3961 gcggggattt ccgccggcgt attttgtgga gtaaaggtag taatcatgga actcaagttg
     4021 attggacaga tgcttcggcg gtggtcatgg ctgttggtca tcggtatcct ggttggtggc
     4081 ggcatcggtt acgcgattag catctatcag acgccggaat acctggctcg caccaagatt
     4141 ctggtgatgg agccgttggg ggataatgcc agcaacgtca ataacctcaa cgacaaggag
     4201 ttggccgaga cctacatgca gttgttgacg acccagccga tcctggagtc ggtcagccag
     4261 aaggtggggg tcaaggttcg cgatacgcag atcaaggcca cccgcgtgcg cgataccggt
     4321 ctgttggaag tgtcggtgcg cgataccgat gcgcaacgcg ccgcgctgac ggccaacgtg
     4381 ctggttgagg tgctgatcga gtacaacgaa gcgctgcaga agtcgcgctt ctcgtcctcg
     4441 gagcagagcc ttgagttgca ggtcagcgag atcaagagcg agttagggcg gctggagagc
     4501 aacgtcgagc agaaatccga gcagacgctg gaagagcagc gccaaacagt cgaacaaaag
     4561 aagaccgaac ttgagcagca gatcttcacc ctgcagggcg agatcgcccg gctgcagcag
     4621 gatatcgacg acctgacgcc ggaagcgctg cccaaccagt tgccggcgcc gctgacgctg
     4681 gagcagcgca atcaactcaa cgagaagcgc acccagctgg cgcagaagga gttccagctt
     4741 gatctggcga aagagtccta ctcccggttg gcggcgccta ccggcactgc ggcccagaat
     4801 gtgcaggatc caaccaatct gcaaaatctg gagcaagcca atctggagct ctatcggcag
     4861 ctctattcca cgctgttgag caactacgag gcggtgcgcc tggctcgtct gcagaatacg
     4921 cctaacgtgg tgcaggtcga gaaggccatg ccgccgacca tccccattca ggccgggccg
     4981 ctgcgcaaca ttatcctggg cgcggttatt ggtctcttgc tgacggggac tgtcgcgttc
     5041 atcctggaat acatggatga tacgttgaag tcaccgcagg atatcgccaa cgtgctgggc
     5101 ttgccggtgg tcggctatgt cgtcaatgag tcggggatgg acaagaccga cggcacaccc
     5161 tatgtctcgg cgaacccacg ttcacctatg gccgagacgt tccgcacgct gcgcaccaac
     5221 ctggagttcg ccagcgtgga caagtccttg aagacgattc tcatcaccag ccccggcgcg
     5281 agcgagggca agacgaccgt ggccaccaac ctggccgcgg tgatggccca ggccaacaag
     5341 cgggtcattc tgctggaggg cgacctgcgc cgtccgcgcg ttcatcgcgc tttggggatg
     5401 tcgaatcaga tcggtctgag cgacgtcttt cgcgggcaga tggatattcg cgacgtggct
     5461 cgctattcaa aggtgaaaga cctggcagtg atcaccagtg gcagcctgcc gccgaacccg
     5521 gccgaactgc tcggctcggc gcgcatgacc cagattctgg ctcgactggt ggattcggcg
     5581 tcggtggtca tcatcgacag cccgcctttc gtcgtggccg actcgacggt gctggcgtcc
     5641 aaggtcgacg gcgtgctgct cgtcattcag ccgggcaaga cccacgccga ggcggccaag
     5701 acgatgttgg cccagctgga tcgggccggc gcccacgttg ttggtgtggt gctgaaccgg
     5761 gtctcgcgca agaccagcag ctactatggg tattatcgtt atgagagcga tcccatctac
     5821 aacggtgaag acgcgtcatc ggaagatagc gctcgacgca aggggctggc ttcgctgttt
     5881 agcggtaacg gaaagaacat gaacgggaag ctggaggaaa aaaccgcgtc ctgatgacga
     5941 aggcggatta tccgcggtga tgggggtcgc cgaaaatcgc aatgtcgtga tcggcggctc
     6001 cccttcccgg tttcccgatg cccgtaaggg cagagtgatt agaggtattg gtcaattgca
     6061 agttgtatcg ccggcagata cgtcttgtaa cggatagtag agtagatatg caaagcagtg
     6121 cgccgtataa atccattcct tccagccgcc aatcggcggc cgatgcgtcg gaaatccccg
     6181 accgttgtcc gtttctcggc actgccaccg atccggctat ggcacatgaa ttcccgtctg
     6241 acgccaatcg gtgctatacc acacgtcttg gggtgcctgt cagcacgatt catcaggaga
     6301 actactgcct gtcgtcccga tttgagagct gccccgttta tcgggaacgc atcaagcggg
     6361 ccggcggaga gacgattgtt cccccggcgg tgatcgccac gatcgcggcg gccagcccgc
     6421 tggtatggca tgatgaagcg catgatgaag ccgctgctgc cgatcctgcg cctgttgaat
     6481 cggcgatgaa tggggccgca tcgacgccgg ttgatgagcc ggtggatacc gccgcgccgc
     6541 tgctgttccc gtgggaaatg cagaccggcg cccctcatcc cgattttcag aatgatccgg
     6601 tggccaagcc ttatcgccgg caatcgcgaa gcatcagcct gcgaccggtg ttgattggat
     6661 tgctgttgct ggccttgatc ccgttggtgt ggtggctatg gaccaccgtc cgcccggggg
     6721 cctctaatga ggttagcggc gcgccaggcg cgatcgtcac attaccgacg gtggtgggcg
     6781 cggggaatgg cggcgaggcg ggcggcgatc ggggcggtac ctcagatctc ccgccggccg
     6841 gcggggagtc gggggccatc gccggtggcg cggccgacga gccgacgatg gaaccaacgg
     6901 ccgaacccac ggcgaccgat ctggagaaga tcgccgccac ggcgacggcc ctgtttgcga
     6961 acgcgacgcc cgtgaccgaa tgtgtggccc catcatggtg ggtagcctat ctggtggagg
     7021 agggcgacac gatcgaggcg ttggcggcca cgcgcggtat taagcccgaa gcgctgatcg
     7081 tcgcaaattg tttggcgggt cctgacctgg agccgggcct gacgctcttg ctgccgccgg
     7141 tgggagtgat cgcctcgttg ccggaggcat cgcccacgcc gaccgccacc gcaacccgcc
     7201 ggccgcaagt gccgggattg cccacgcgcc ggccgatttt tctcccgaca ccgacgttcc
     7261 cggtcattat catcttgacc ccgcaggtgc cggtcatcga gccgaccgac ccgcccgacg
     7321 aagaaccgcc ggttcgggcg accagaacgc cttcggtctc gccgactgcg acggccccca
     7381 atccgtttcc gcaggccacg ttgacgcccc ccgggtttat tacgtcaacg ccgccgggat
     7441 ttatcacatc gacgccaccg ggattcatca cgtcaacacc accgtcacct ggtggaacga
     7501 tccctgtgac gccgacgatg acgccgcccg cgcccggacg gtaggcccgg cgaatgatat
     7561 tgtttacata gaagcgatcc gatttgatcg cgcttaagaa tggcatttca tcagggaagt
     7621 ccatgaacag cacaacgatt ggccatcacc aagcgcaaaa tattgatatc aaccttcgcc
     7681 gtctcgacgc ggccgtgctg gccatgacgg ccccttttct gctggcgccg gcgtatttct
     7741 ccccggcgtt gtcgggcatc gccctgtcgt tgctatcctt tccctatatc atccgactgg
     7801 ctttcggcaa gccgctctct cggccgacga tcgtcaatct gccggtgggg cttctcgcat
     7861 tcgtcttcat gccgttggct ttgctgatga gtccggcgac ctgggggacg agttgggagc
     7921 gggtgctcac cctggcctgg tctattgccc tgttttttgc cctcgtcagt tggcccggct
     7981 cagggagcaa ggctgattta cgaacgcgat tcagccgccc gacgctcatc tacctgggat
     8041 tgggaacgct ggtggcgcta ctcggcctgc tggccatgcg cagtgtggac aagctgttct
     8101 ttttgcccca gacgg
//
LOCUS       DAOMQG010000127         8087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3843_length_8087_cov_6.625872, whole genome shotgun sequence.
ACCESSION   DAOMQG010000127 DAOMQG010000000
VERSION     DAOMQG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_8087_cov_6.625872"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..562)
                     /locus_tag="PLC06_08530"
     CDS             complement(338..562)
                     /locus_tag="PLC06_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90529.1"
                     /translation="MNSEFGRFVLVFLFTWAMLMTGLVLILWATRPTAQPPVIIAQAQ
                     PQDEMGCSLFLILLLVALIALLVFMVLSNL"
     gene            1339..4767
                     /locus_tag="PLC06_08535"
     CDS             1339..4767
                     /locus_tag="PLC06_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRO90530.1"
                     /translation="MLTWPALRERMRLNWRLSPDDLDAALLLGLPHIAPPADLSPAAP
                     ILAAETTQWAAAYLRGDSADSLLLRRRLFRAVYCDWPDLSHVLRLLAAHHARLFFGHD
                     DPEAHTARQTSEAYVHVAGMLSLWRLRHVWIEESIRQIQPGKYAIAAEKIGIPWATNL
                     DADLLEQQIISEQQRWSGDDGDSFLTPGRFDIESRMRALNRLNRRLSEALDDEFAPAG
                     RPRLELMSMMPGITLHYAQSRRFTLEEWLQQLPQLHLRLFCATESDCYRALGVVHRVT
                     PPIRPGDNNRFRNFDDYIARPQNNGYRGLQAICQWPAGSKGALIRWHILTDSMHDLND
                     WGAMSYESGTQRTSLGLSRSLKRLSRSLERSIGENILPYIRTHDLNSGPDPIYCFTPE
                     GEIVLLDKGSRLLDFAYRIHTQLGHQATRIEVNETTKPLETELRNGDLVRIAYDPLAA
                     GPDFSWQEYVTWERARTNIRTELRRRAAQVHAGRSNFMAELIRWIDIYRRDADRRRAE
                     RDGKLYKLPIPASAEIDHFLDRVVARRRLDSRATLYELLVKHPRLTRDLAQRLLSEGI
                     IPSLSMPGGQPLRRRVADIALCPTCRPTPMDTICAYRRYIYNGDMIIIHRPGCPAISS
                     AADMVDLAWDENHSDRWPLYQFKIGCDDEDGLLDRLLALVYDTPYTYLYQVKASVSEK
                     QRADIQLIVAVKLPQLCSDLALMLVEAGFEVTYSPHDSARRSPVQREDRSRELDNPFT
                     LDQVSDWRFYNRDMILRELLDWAGDPHPSSPVKLLHGQQRIGKSSVAARLANGLAEQN
                     ASLSRSAARTIPLNIDFSSVRLEDPAAVADLLTQRIGGRLDFCHVKRPPDTDPILWLD
                     RQLIETESVLGEARLLIILDEFDGQFDRLAEHGSRFTLPDRLWGLIRGHAQKIRWLLI
                     AQDAHLEDPRYEGMFSRLLVAPRVHVDPLDNLYARQLIVNPIIERDYRFTAALPDSPD
                     IPARVLDLAAGNPYIIHLIGGELLERLGRGNRRVITDNDLIWTTQLLLGRPAMLDHYT
                     KPLLTTPTRHRIALCLAAGIPVGGRLALSAVSDALVNRRHLMDEVTLHQELTYLEKIG
                     VFTVTPNEFGDRVVSLPVQLIHHLLRRWINDPANHTWSLDLLDTPPNPDLYPGG"
     gene            4773..6224
                     /locus_tag="PLC06_08540"
     CDS             4773..6224
                     /locus_tag="PLC06_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO90531.1"
                     /translation="MYGNPLLNPFTELLATGAFLTVGHRKLLAELAGVISQDEYEGRQ
                     LHGMPNVGKSPLLRRIAGDGFLSPLHDQFLEPYRDQPQRVFTFYVSGWIKSIHPIALI
                     YKEYTRRFRGYSAWLPTIDEAFVQNPLPELGDLAGPDSNDIEQALDLLESHLLAMRDA
                     GIRVVMVLDDFDSDWAFGALTPNEAKRITAWRQYLSLICATERLLEDVNPEARKIMSP
                     LFKRLTMRQVANLSPGEVKALMDNILDGAKEKLPPNDIEQLILYSGGFPYLLLVGGQT
                     LWELRDRLRILSSPDRPLEGSVLAIWRASLSQEFSRVFSVYYDWMSPERQALLLEMAR
                     AGSSVALSRFDEGEARSHLSWLEKYGLIRINEEQDQGTLFSPLFRDFLLSLDAQTPAE
                     ETGQMPALPRQQADLYTTFRGRPNEPLSYTELGSAVWGWPAGRRDEEIDEDEKRKIHL
                     AIHKLRAELERTGRGERIVNVRKRGYRYEPGAG"
     gene            complement(6326..7081)
                     /locus_tag="PLC06_08545"
     CDS             complement(6326..7081)
                     /locus_tag="PLC06_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HRO90532.1"
                     /translation="MNPLDLEKVRLFVNDNIDDFHQRKLASVQRLTLTKLLAKNPYLF
                     KAKNINSAGELIAGLLDAFLSSSEEKLFGDFLESLAVFVAGLTCNGHKSAAQGIDLEF
                     IDNATHYIVSIKSGPNWGNSSQHKKLAQDLRDGVTRIRQSRTGLNIQPVLGICYGKTR
                     TAYTKEGYLKVVGQNFWYLISGNPDLYLDIIEPIGYRAKEHNDKFMAGRAALENKLTG
                     EFIAAYCDSSGAINWEQFVRANSENRDLDELLP"
     gene            complement(7068..7937)
                     /locus_tag="PLC06_08550"
     CDS             complement(7068..7937)
                     /locus_tag="PLC06_08550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRO90533.1"
                     /translation="MDYVDRILLGDCRSVLAEMPDNSVDLIFTSPPYADQRRHTYGGI
                     KPDEYVDWFMPRAEQFLRVLKPTGTFILNIKERVVNGERHTYVLELILEMRRQGWLWT
                     EEFMWHKKNSYPGKWPNRFRDSWERLIQFNKQRHFNMYQEAVMVPVGDWAKTRLNKLS
                     ETDLIRDESRVNSGFGKNVSNWVGRDMVNPTNVIHMSTECSNRNHSAAFPLELPTWFI
                     KLFTAEGDLVLDPFVGSGTTVEAAKLLGRHFCGIDIDPEFHLLASSRVMEKQARLLES
                     PEPYSVNGKDEPA"
BASE COUNT         1636 a         2509 c         2283 g         1659 t
ORIGIN      
        1 aagaaacccg gttccccggt cgaaatttaa aatgatacga tggcggagcg gccgcacagc
       61 caacaccccc caccggaatg cacaactgtc aaattgcact aacgacaaga cgggatttta
      121 gtcgaattgg gggagagggg caaacgagtg acgagtgacg agtgacgagc gacgagtgac
      181 gagcgcgctc cagcggccgg tcgccggtcg tcccttccat gaataaagcg caggctcccc
      241 tcgcctgcgc cttatgcctg agatgtgtcc tccccgaaca cacgctcatt tgatcaatcg
      301 gccgccgccc cgcgcgaagc ggcggccgtc aggaatgcta cagattcgac agaaccatga
      361 acaccagcag agcgatcaac gccaccaaca gcagaatcag aaacaagctg caacccatct
      421 catcctgcgg ctgagcctgg gcgatgatca ccggcggttg ggctgtcggg cgcgtggccc
      481 acaggatcaa caccaacccc gtcatgagca ttgcccatgt gaacaagaac accagaacaa
      541 acctcccaaa ttcgctgttc atcgcctggc acctcccaat gccttttgtg cggtggacat
      601 tcgacggcca tcggccatct tcacgctttc atctctacac cgtcacgcgc tgtgcggctc
      661 gagcgggcca tcgacccctc ggccgcgcct gtaaagagaa aggtggcccc ggccgactgg
      721 aaagccgggg ccttgtgagg tcgattggcg cgtcgcgaac ccttgccgcg acaaatccca
      781 atcgtccaac tcactcagat tgttaaggcg ttgaattggc gagggtgtga tgaagagagc
      841 gatgaacctt tccagacgct taggaaagat atttcctttc tttctaactg ataagaatca
      901 taacggatcg tcagggtaaa agtcaatagg ggatacccct taataggaag aattggctag
      961 tggatcgtcc tggtggatag cgaaaatgcc atcccggctg cgagcctctc caatatgtcc
     1021 ggttttggaa accaaatggt caccgatagt tcactgattg gaaacattga agaaataaat
     1081 gaatgaatat tgccgggtca ttgacattcc ggaaagatta gtatagaatg agtactgtct
     1141 tcaaccgcca tggagacagg ctctgttggg aacggggcag tgcggccaac gtcgaaatcg
     1201 gccgcaattc aatccagcga cgcatcactc ggtcaaccgg aacaaaatca atatggacag
     1261 caagcgggtg gaggcagcgg ccgccccacc atcgaacgac tcaatggcga cgtcgcctcg
     1321 aatgggggta gggctttcat gctgacctgg cccgccctgc gcgagcgcat gcggctcaac
     1381 tggcggctgt cgcccgacga cctggacgcg gcgttgctgc tggggctgcc ccatattgcc
     1441 ccgcccgccg acctctctcc cgccgcccca atcctggccg ccgaaaccac ccaatgggcc
     1501 gcggcctacc tgcgcggcga ttcggccgac tcccttctgc tgcgtcgccg cctctttcgc
     1561 gccgtctact gcgactggcc cgacctgagc cacgtcctgc ggctgctggc cgcccaccac
     1621 gcgcggctgt ttttcgggca tgatgatccc gaagcccaca ccgcccggca aaccagtgaa
     1681 gcctacgtcc acgtggccgg aatgctcagc ctgtggcgct tgcgtcatgt ctggatcgag
     1741 gaaagcattc gtcaaattca gcccggcaaa tacgccatcg ccgcggaaaa gatcggcatt
     1801 ccctgggcga ccaacctcga cgctgatttg ctggagcagc agattattag cgaacagcaa
     1861 aggtggagcg gcgacgacgg tgatagtttc ctgacgcccg gccgattcga catcgaaagc
     1921 cgcatgcgtg cgttgaaccg ccttaatcgc cggttgagcg aagccctcga tgatgagttc
     1981 gccccggccg gccggccgcg gctcgagttg atgtcgatga tgcccggcat cacgctgcat
     2041 tatgcccaat cccgtcgttt taccctggag gaatggttgc agcaattgcc gcaacttcat
     2101 ttgcgtctct tttgcgccac cgaatccgat tgctatcgcg cccttggcgt ggtacaccgc
     2161 gtcaccccgc ccatccggcc aggcgacaac aaccgcttcc gcaacttcga cgactacatc
     2221 gcgcggccgc aaaacaatgg ctatcgcggt ctgcaggcca tctgccaatg gcccgccggg
     2281 agcaaagggg cgctcattcg ctggcatatc ctgaccgact caatgcacga tctcaacgat
     2341 tggggggcga tgagttacga gagcggcacc caacggacga gtctggggct gagccgctcg
     2401 ctcaagcgcc tctcccgatc gcttgaacgc tcaatcgggg aaaatatcct cccctacatt
     2461 cgcacccatg acctgaatag cggtcccgac cccatctatt gcttcacgcc cgagggcgag
     2521 attgtcctgc tggacaaagg cagccgcctt ctggacttcg cctatcgcat ccatacccag
     2581 ctgggccatc aggcgacgcg cattgaggtc aacgagacca cgaaaccgct ggagaccgag
     2641 ctgagaaacg gcgatctcgt tcgcatcgcc tacgatccgc tggccgccgg gcccgacttc
     2701 tcatggcagg aatatgtcac ctgggagcgc gcgcgcacca atattcgcac cgagttgcgg
     2761 cgacgggccg cccaggtcca tgccggccgg agcaatttca tggcggagct gatccgctgg
     2821 atcgacatct atcgccgcga cgccgaccgt cgccgcgccg agcgagacgg gaagctctat
     2881 aagctgccca tccccgcttc ggccgaaatc gatcattttc tggatcgggt agtggcccgt
     2941 cgccggctgg atagccgggc caccctgtat gaactgctgg tcaagcatcc ccgattgacc
     3001 cgcgatctgg cccaacggct gctctcggag gggatcatcc cttccctgag catgcccggt
     3061 ggccagccgc tccgccggcg cgtggccgac atcgccttgt gcccgacctg ccgcccgacc
     3121 ccgatggaca ccatctgtgc ctatcgtcgt tacatctata atggcgatat gatcatcatc
     3181 caccggcccg gttgcccggc catctcatcc gcggccgata tggtcgacct ggcgtgggat
     3241 gagaaccaca gcgaccgttg gccgctctat cagttcaaaa tcggctgcga cgacgaagac
     3301 ggcttgctcg accgcctgct ggcgctggtc tacgacaccc cctataccta cctctaccag
     3361 gtcaaggcgt cggtaagcga gaagcagcgg gccgacatac aactgatcgt cgcggtcaag
     3421 ctgccccaac tgtgttccga cctggccctc atgttggtgg aggccgggtt cgaggtgacg
     3481 tatagccccc atgacagcgc ccgccgaagc ccggtccagc gggaagatcg atcgcgtgag
     3541 ctggataacc ccttcacact cgatcaggtc agcgactggc gcttctataa tcgcgacatg
     3601 atcctgagag agctgctgga ttgggccggc gacccccatc cgtcttcgcc ggttaaactg
     3661 ctccacggcc agcagcgtat cggcaaatcg tccgtcgccg cgcggctggc caacggattg
     3721 gccgaacaaa acgcatctct ctcgcgttcg gccgcccgga ccatcccgct caacatcgat
     3781 ttctccagcg ttcgccttga ggacccggcc gccgtggccg atctgcttac ccagcgcatc
     3841 ggcgggcggc tggatttttg tcatgtcaaa cggccgcccg atacggatcc catcctctgg
     3901 ctcgatcggc aactgattga gacggaatct gtgctgggcg aggctcgcct gttgatcatc
     3961 ctcgacgaat tcgacgggca attcgatcgc ctcgccgaac acggctcgcg cttcaccttg
     4021 cccgacaggc tctggggctt gattcgcggc catgcccaaa aaatacgctg gctgctcatc
     4081 gcgcaggatg cccatctcga agaccctcgc tatgaaggga tgttctcccg tctgctggtt
     4141 gcgccgcgcg tccacgtcga tccgcttgat aacctgtatg cccgacagct catcgtcaac
     4201 ccgatcatcg agcgggacta tcgtttcacc gctgcattgc ccgactctcc ggacattccc
     4261 gcgcgtgtcc tcgacctggc cgccggcaac ccctatatca tccatctgat cggtggggag
     4321 ttgctggaac ggctggggcg tggaaatcgc cgggtcatca ccgacaacga cctcatctgg
     4381 accacccagc tcctgctcgg ccgcccggcg atgctggatc attacaccaa accattgctc
     4441 acgaccccga cgcgacaccg gatcgccctc tgtctggccg ccgggatccc cgttggcggc
     4501 cgccttgcct tgtcggccgt cagcgacgcg ctggtcaatc gccgtcacct gatggatgag
     4561 gttacgctgc atcaggagtt gacctatctg gagaagatcg gcgtgttcac cgtcacgccg
     4621 aacgaatttg gcgaccgggt cgtctccttg cctgtccagc tcatccatca cctgctgcga
     4681 cgctggatca acgatccggc caatcacacg tggagcctgg atttattgga tacaccccca
     4741 aaccctgacc tgtatcccgg aggttaatca cgatgtatgg gaatccactt ttaaacccgt
     4801 tcacagaact tcttgccacc ggcgcgtttc tgaccgtcgg tcacaggaaa ctgctcgccg
     4861 aactcgcggg cgtcatctct caggacgaat acgaaggtcg ccaactgcac ggcatgccca
     4921 atgtgggcaa aagccctttg ctgcgccgga tagccggcga cggcttcctc agtccactgc
     4981 acgatcagtt tctggaaccc tatcgcgatc aaccacagcg cgtgttcacc ttttatgtat
     5041 ccggctggat caagtccatt catccgatag cccttattta taaagaatat acccgccggt
     5101 ttcggggcta cagcgcgtgg ttgccgacga ttgacgaggc gtttgtccaa aatcccttgc
     5161 ccgagcttgg cgacctggcc gggccggatt ccaacgatat tgagcaagcg cttgacctgc
     5221 ttgaatccca tctgctggcg atgcgcgatg ccggaattcg ggtcgtcatg gtgctcgatg
     5281 attttgacag cgattgggcc ttcggcgctc tgactcccaa tgaggccaaa cgcatcaccg
     5341 cctggcgcca atacctgtcg ctcatctgcg ccacggaaag gctgctggaa gatgtgaacc
     5401 ccgaagccag aaagatcatg tcgcccctct tcaagcgcct gaccatgcgc caggttgcca
     5461 atttgtcgcc cggtgaggtc aaagccctga tggacaacat tctggacggc gccaaagaga
     5521 agctgcctcc aaatgatatc gaacaactga tcctgtattc gggcgggttc ccttatttat
     5581 tgctggttgg cggccagaca ctttgggaac tgcgcgatcg cctgaggata ctgtcgtctc
     5641 ccgatcggcc gttggaaggc tccgtgctcg ccatctggcg cgcgagtttg agccaggagt
     5701 ttagccgcgt tttcagcgtc tattatgatt ggatgtcgcc ggagcggcag gcgttgctgc
     5761 ttgagatggc gcgcgccggt tcttcggttg cgctgtcgcg gtttgatgag ggcgaagcgc
     5821 ggagccacct gagctggctg gagaagtatg ggttgattcg aatcaatgag gaacaggatc
     5881 agggcaccct cttctccccc ctctttcgcg atttcctgct gtcgcttgac gcccaaacgc
     5941 cggccgagga aacggggcaa atgcccgccc tcccccgcca gcaagccgac ctctacacca
     6001 ccttccgcgg ccgccccaac gagccgctca gctacaccga actgggcagc gccgtctggg
     6061 gctggcccgc cggccgccgc gacgaggaga tcgacgagga cgagaagcgc aaaatccacc
     6121 tggccatcca caaactgcgc gccgagctgg agcgcaccgg ccgcggcgag cgcatcgtca
     6181 acgtccgcaa gcgtgggtat cggtatgagc cgggggcggg atgagaaggg gagcagggga
     6241 gaaagggaga aggggagaaa cccggtttct agaggtaacg ctcttgcggc ggtcggcggt
     6301 cggcggtctg tggtctgtgg cccgcctacg gcaacaattc atccaggtcg cggttctcgc
     6361 tattggcccg gacgaattgc tcccaattga tcgctcctga actgtcgcag taggccgcga
     6421 tgaactcgcc ggttaattta ttttccagcg cagcgcgccc tgccatgaac ttgtcgttgt
     6481 gttctttcgc gcggtagccg atgggttcaa tgatgtcgag ataaaggtca ggattgccgc
     6541 tgatcagata ccagaagttt tggccgacga ccttgagata gccttctttt gtgtaagcgg
     6601 ttcgggtttt cccgtaacaa atgcccagta cgggttgtat attcagaccg gtgcgggact
     6661 gacggatccg cgttacgccg tcccgcaggt cctgggccag ttttttgtgc tgcgaactgt
     6721 tgccccaatt cgggccggac ttgatggaga cgatgtagtg cgtggcgttg tcgatgaact
     6781 ccagatcgat cccctgggcc gctgatttat ggccgttgca tgtgagtccg gcgacaaaaa
     6841 cggccaggct ctccagaaaa tcgccgaaca gcttttcttc cgaggacgag agaaaagcat
     6901 ccagcagccc cgcgatcagc tcgccggccg agttgatgtt cttggcctta aacagatatg
     6961 gattttttgc cagtaatttg gtcagggtca gacgctgcac ggaagccagc tttcgctggt
     7021 gaaagtcgtc gatgttatca ttgacgaaaa ggcggacttt ttccagatca agcgggttca
     7081 tcttttccgt tcaccgaata gggttcggga ctttcgagca gccgggcctg cttttccatg
     7141 acgcgggatg aggccaatag atggaattca ggatcgatat caatgccgca aaaatgccgc
     7201 cccagcagct tggcggcttc aactgtggtg ccggagccga cgaacggatc gagcaccagg
     7261 tcgccttcgg ccgtaaacag cttgatgaac cacgtcggca gttccagcgg gaaagcggcg
     7321 ctatggtttc gattggagca ttcggttgac atgtggatga cgtttgttgg attcaccatg
     7381 tcacggccga cccagttgga cacatttttc ccgaagccgc tattgaccct ggactcgtcg
     7441 cggatgagat ccgtttcact cagcttgttg agtcgcgttt tggcccagtc ccccaccgga
     7501 accatcaccg cttcctgata catgttgaaa tggcgctgct tgttgaactg gatcagtcgt
     7561 tcccatgaat cgcggaatcg gttcggccat ttgcccggat aggagttctt tttatgccac
     7621 atgaactcct cggtccaaag ccacccctgc cggcgcattt caaggattag ctccaggaca
     7681 taggtatggc gttcgccatt gacgaccctc tccttgatat tgaggatgaa tgtacccgtg
     7741 ggcttcagga cgcgcaggaa ttgttcagct ctgggcatga accagtcgac gtactcgtcg
     7801 ggcttgatgc cgccataggt gtggcggcgc tggtcggcat aggggggcga ggtgaatatt
     7861 aaatccaccg aattgtcggg catttccgcc aacacggacc ggcagtcacc caagagtatc
     7921 cgatcaacat agtccatggc tggtctctca gaacatctat tctagctcgt tgttgtagat
     7981 cggcaacatt tcttccgagg ctcaattgcc ggatcgcccc aaaaccagcc cttccccctc
     8041 gtccaccacc cccacccagc ccggcgggat gagcgtcgtc gtgtcat
//
LOCUS       DAOMQG010000128         7978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3901_length_7978_cov_10.226934, whole genome shotgun sequence.
ACCESSION   DAOMQG010000128 DAOMQG010000000
VERSION     DAOMQG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_7978_cov_10.226934"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLC06_08555"
     CDS             complement(<1..592)
                     /locus_tag="PLC06_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRO90534.1"
                     /translation="MDFENVLTLIQKELRDAFRNRWFLLYAIAFAGLSLALAWFSVSG
                     AGSYGVAGFGRTTAGLINLILLIVPLMGLTLGALSLAGEREKGTLIYLLAQPISSGEL
                     LLGKFVGLALALTAALLIGFGLTGVMMAVAGGGGDFGIYLTLLVLSVLLAIVSLSLGF
                     LISAAVKKAATAVGLALFLWLILVYFGDLGLMGTAVV"
     gene            complement(643..1479)
                     /locus_tag="PLC06_08560"
     CDS             complement(643..1479)
                     /locus_tag="PLC06_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90535.1"
                     /translation="MTKRFGRFTAVDDLSFTVGAGEAVALWGANGAGKTTAVRCLLNL
                     FPFEGEILVNGLDARRAGKDVRRLIGFVPQELSFHDDMSVAETLIFYARLKKVPDGFD
                     FQPLLEQVRLVPHVHKRVGELSGGLKQRLALAAALLSDPPILVLDEPTASLDIRSREE
                     FLVLLRSLKSKGKTMIFSSHHLEEVTALADRVLLLEGGRLVVDAPPDQLERRLGWETT
                     LHLYLPETVIDPAVEHLEGLGMPVSRNGRGVRVHVAPGAKGKVIRVLHEAGIEVDDFS
                     IE"
     gene            complement(1499..2926)
                     /gene="nosD"
                     /locus_tag="PLC06_08565"
     CDS             complement(1499..2926)
                     /gene="nosD"
                     /locus_tag="PLC06_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HRO90536.1"
                     /translation="MKYFRLLLILFLLAAIPHVAWADEIIRVPAEELAAAVAAAQPGD
                     TIEVTGGVFNGNLVIDKPLTLIGIDNPILDANFFGTVVMVTAPDTTVRGFTLRNSGNK
                     LITEDAGVNVGAPGALIEDNILEDVLFGLYLHSSPNSTLRNNHITGKDLDIARRGDLI
                     RIWESNDVLIEGNVTINGRDAVLWYSQRLTLRNNEFTHGRYGLHFMYDDDAIIEGNRL
                     THNSVGAYLMYSRRTVVKDNLLAFNRGPSGYGIGLKDVDDASLIGNRILDNRVGAFLD
                     ASPRELNSVGIIQGNLFAYNDIAIEMLPSVRHNMISDNSFVENEQQVVVAGGGQLRDN
                     EWTVRDRGNYWSDYAGYDADGDGRGEVSYQAQRLLDNLLGSRPELRLFLYSPVVNAVD
                     FASRAFPIVRPQPILVDDAPLTQPLPPAGAPLPIVGNEGALWPVLLLPLVLLFLYAFM
                     RLGQRRYHMPASPALMGREELGHGD"
     gene            complement(2986..3633)
                     /locus_tag="PLC06_08570"
     CDS             complement(2986..3633)
                     /locus_tag="PLC06_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRO90537.1"
                     /translation="MSKLIEKIIGPRTPKELSEEDRQRFRLPSILFGIAAFLLLVSIF
                     FPYWGMTLKAPQYPGGLSVQVFVNRMEGDVKEIDGLNHYIGMRPLDEAAQLERSLSFI
                     VITALVLLTLAAIFIRSPWTLLMALPAILWPLIFLGDMYYWMRNFGLNLDPKAPLSSA
                     IKPFVPPILGSGFVGQFETIATFHLGIWMAFLASALILAGLYYQRRAYKPLVDKV"
     gene            complement(3746..5785)
                     /gene="nosZ"
                     /locus_tag="PLC06_08575"
     CDS             complement(3746..5785)
                     /gene="nosZ"
                     /locus_tag="PLC06_08575"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRO90538.1"
                     /translation="MTLNRKSTRPIMLITVLMIVAAMAVLIVSCRDNNGGAAAVPQGA
                     GATEDINVIAEARGLTPGDIVNAVKTYTPSGKHDEYYLFASGGQSGQVYVIGLPSMRI
                     IKQIAVFTPEPWQGFGYGELGTLQVLREGGLAADGTPLTWADTHHPGLSETNGEYDGQ
                     YLFINDKANARVAVIDLRDFEVKQIVKNPITLSDHGGTFVTPNTDWVIEGGQYGMPLG
                     FKYSSIENYEEDYQGMITFWKFNREAGRIATDQSFAIELPPYWQDLCDSGKLVSDGWV
                     FCNSINTELATGGVELGNPPFESGASQNDMDFLHIINLKKAAEVYAAGKFDLINDFPV
                     ISLDTAIGEGLLYFAPEPKSPHGSDVTPDGEFVIVAGKLDPHVTVFGFDKIMAAIEAG
                     TSDTDQYGVPILDFDSVMETQVELGLGPLHTQFDNKGYAYTSLFLDSAVARWSLGGSF
                     DELNEDPPWTLVTKTPVHYNIGHLVTAEGDTVSPDGNWLVAMNKWAVDRFMPVGPLLP
                     QNFQLVDISQPGTNMPLVYDMPLPDGEPHYAQIIKADKIKPWDVYPEIGWDPHTQSVR
                     ADAPQQGTERVERDGNNVTVYMTAIRSHFTPERVEIKKGDHVTWHITNVERALDATHG
                     FTIPAYNIHISLEPGEVASFEFDAVQDGVFSFYCTEFCSALHLEMMGYMTIDPGE"
     gene            complement(5971..6618)
                     /locus_tag="PLC06_08580"
     CDS             complement(5971..6618)
                     /locus_tag="PLC06_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRO90539.1"
                     /translation="MAHGRIYEVEPGDFFFHQGEDSTMLYVILSGRVKLSQVTSEGDQ
                     VIIDYFGSGEGLGIAMALNKKPYPLTAEAIEPCTAAGWGRETMLDLMKENAQLAINGM
                     NMISQRFTQMQHRFQELATQRVERRVARALLRLVRQFGRRTEEGVLIDISLSREDLAQ
                     MTGTNLYNVSRILSKWESSGLITTGRKQVTLVNAHELVIMAEDIPQPGSRAPKTD"
     gene            6900..>7978
                     /locus_tag="PLC06_08585"
     CDS             6900..>7978
                     /locus_tag="PLC06_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HRO90540.1"
                     /translation="MKNRFSRPYLIMTAALLALFVLVLGGQRSAQAQDAQWLGEFWNN
                     RDLNGPVVLTRWENSIDFNWFDGSPDPRINSDNFSARWTRTVNFPAGTYRFYATMDDG
                     MRIWIDNVLVIDSWTHSQEHTMTVDRFMNGNHSIRIDYFEAGGAATARFNWRLINNAG
                     GGQFFPNWRAEYFNNMTLSGSPSLIRDEATISIDWGDGSPGPGVNSDFWSARFTRQFT
                     ARPGQYRIILTSDDGSRLFINNQLIIDNWRAQAATSVSTDFFTNGGTMDVRVEFFDRT
                     GGALLRADLITISGGLITGQPTPPTGEGGVCATAPAGMQGQSTATTVLNIRRGPSTQF
                     ETMGTLSRCQIVPLSGFRNADSTWI"
BASE COUNT         1616 a         2321 c         2401 g         1640 t
ORIGIN      
        1 tgaccacggc cgtgcccatt aagcccagat cgccgaaata gaccagaatg agccataaga
       61 acagggccag gcccacggcc gtggccgcct ttttaaccgc ggccgagatc agaaagccca
      121 ggctcaggct gacgatggcc agcagcaccg ataggaccag caaggtcagg tagatgccga
      181 aatcgccccc gccgccggcc acggccatca taacgccggt cagcccgaag ccgatgagca
      241 gcgcggccgt cagcgccagc gccagcccta cgaacttgcc cagcagaagc tcaccgctgc
      301 tgatgggttg ggccagcaaa tagatgagcg tgcccttctc tcgttccccg gccaggctca
      361 gcgcgcccag cgtcaacccc atgaggggca cgatgagcag gatgagattg atcagcccgg
      421 cggtggtgcg gccgaagccg gccacgccat agctgcccgc gccggacacg gaaaaccagg
      481 ccaaagccag cgacaacccg gcaaaggcga tagcgtagag caggaaccag cgattgcgga
      541 aggcgtcacg caattccttt tgaatcaacg tcagcacatt ttcaaaatcc atgatcacca
      601 cccacacgaa tgaagcccgg cgaccggctg gccgccttgc ctctattcga tggaaaagtc
      661 gtctacctcg atgcccgcct catgcaggac gcggatgacc ttgcccttcg cgccaggggc
      721 cacatggacg cgcacgccgc ggccgttgcg actgaccggc atgcccagtc cctccagatg
      781 ctccaccgcc ggatcgatga cggtctccgg cagatagagg tgcagcgtcg tctcccaccc
      841 caaccgtcgc tccagctggt cgggcggagc gtccaccacc aggcgaccgc cttccagcag
      901 taacacccgg tcggccagcg ccgtcacctc ttccagatgg tgcgacgaga agatcatcgt
      961 cttgcctttg cttttcagcg agcgcagcag caccagaaac tcttctcgcg agcgaatgtc
     1021 gaggctggcc gtcggttcgt caagcaccag aatgggcggg tcggacagca aggcggccgc
     1081 cagggccagg cgttgtttca acccaccgga cagctcgccc actcgcttgt ggacgtgggg
     1141 aaccagtcgc acctgttcca gcagcggctg gaagtcgaag ccgtcgggca ctttctttaa
     1201 ccgggcgtag aagatgagcg tttcggccac gctcatgtcg tcgtggaagc tcaattcctg
     1261 gggtacgaag ccgatcaggc gtcgcacatc cttgccggcg cgtcgcgcat ccagcccgtt
     1321 gacgaggatc tcgccctcga acgggaaaag attcagaagg cagcgcacgg ccgtggtctt
     1381 gccggctcca ttagcccccc agagggccac cgcttcaccc gccccgaccg taaaggacag
     1441 gtcgtctacg gccgtgaacc gcccgaaccg tttcgtcaaa ttcctgactt caatcatatc
     1501 agtcaccgtg gcccaactcc tctcgtccca tcagggccgg cgaagcgggc atgtgatagc
     1561 gtcgttgtcc cagtcgcatg aaggcgtaca ggaacagcag caccagcgga agcaacagca
     1621 caggccacag cgcgccctcg ttgccgacga tgggcagagg ggcgccggcc ggcggaagcg
     1681 gctgggtcaa tggggcatca tccaccagga tgggctgggg tcggacgatg ggaaaggcgc
     1741 gcgaggcgaa atcaaccgcg ttcaccaccg gactatagag gaacagccgc aattccggtc
     1801 ggctgccgag caggttgtcc agcaggcgct gggcctggta ggatacctcg ccgcggccgt
     1861 cgccatcggc gtcatagcca gcgtagtcgc tccaatagtt gccgcgatcg cgcacggtcc
     1921 actcattgtc ccgcaattga ccgcccccgg ccacgaccac ctgctgctcg ttctctacga
     1981 agctgttgtc gctgatcatg ttgtggcgga ccgacggcag catctcgatg gcgatgtcgt
     2041 tataggcgaa cagattgccc tgtataatgc cgacgctgtt cagctcgcgc ggggaggcat
     2101 cgagaaaagc ccctactcga ttgtccagga tgcggttgcc gatcagcgag gcgtcgtcaa
     2161 cgtccttcag gccgatcccg tagccgctgg ggccgcgatt gaaggccagc aggttgtcct
     2221 tcaccaccgt gcggcgactg tacatgagat aggcgccgac cgagttatgg gtcagccggt
     2281 tgccctcgat gatggcgtcg tcgtcataca taaaatgcag cccgtagcgg ccgtgggtga
     2341 actcattgtt gcgcagcgtc agccgctgcg aataccacag cacggcatcg cggccgttga
     2401 tggtgacgtt gccctcgatg aggacgtcgt tgctctccca gatgcggatc aggtcgccgc
     2461 gccgggcgat gtcgaggtcc ttgccggtga tgtggttgtt gcgcagggtg ctgtttgggc
     2521 tgctgtgaag gtagaggccg aacagcacat cttcgaggat attgtcctcg atgagcgcac
     2581 cgggtgcgcc gacgttaacc ccggcgtcct cggtgatcag cttgttgccg gagttgcgca
     2641 gtgtgaagcc acgaacggtc gtgtcgggag cggtcaccat caccactgtg ccgaaaaagt
     2701 tggcgtccag aatgggattg tcgatgccga tgagcgtcag cggcttgtcg atcaccaggt
     2761 tgccgttgaa gacgccgccg gtcacctcga tggtgtcgcc gggctgggcg gccgccaccg
     2821 ccgccgccag ttcttcggcc ggcacgcgga tgatctcgtc cgcccatgcc acgtgaggaa
     2881 tggccgcaag gaggaagagg atgaggagga gacgaaaata cttcattaga aatttggaat
     2941 ttggaattac gaattacgaa ttacgaatcc cttatccata ttatattaga ctttgtcgac
     3001 cagcggcttg taggcgcgac gttggtagta taacccggcc aggatgagcg ccgatgccag
     3061 gaaagccatc catatgccaa gatggaacgt ggcaatcgtc tcgaactggc cgacgaagcc
     3121 ggagccgaga atgggcggca caaacggctt gatggcgctg ctcaacggcg ccttggggtc
     3181 gagattcagc ccgaaattac gcatccagta gtacatatcg cccagaaaga tgagcggcca
     3241 caggatcgcc ggcaaggcca tcagcagcgt ccacggcgag cgaatgaaaa tcgccgccaa
     3301 cgtcaacaac accagcgcgg tgatcacgat gaagctcagc gaacgctcaa gttgggcggc
     3361 ttcgtccagc ggccgcatgc cgatgtagtg attcagaccg tcgatctcct tcacatcgcc
     3421 ttccatccgg ttgacgaata cctgaaccga taatccgccc ggatattgcg gcgctttcaa
     3481 cgtcattccc caatagggaa agaaaatcga caccagcagc agaaaagcgg cgataccaaa
     3541 caggatgctg ggcaagcgaa acctctgccg gtcttcctcc gaaagctctt tcggggtacg
     3601 cgggccgata attttctcaa tcaatttact catgatttcc tctcgccatt gagcattggc
     3661 gcgatacagg aaggggtgag gccggggcga ttgttaggtc gccccagcct ctcacactaa
     3721 ggagtcgtta gttgatggcc ggggcttact cgcccgggtc gatggtcata tagcccatca
     3781 tctccagatg cagggccgag cagaactcgg tgcagtagaa ggagaacacg ccgtcctgca
     3841 ccgcgtcgaa ctcaaacgac gccacttcgc ccggctccag actgatgtgg atgttgtagg
     3901 ccgggatggt gaagccgtgg gtcgcgtcca gtgcgcgctc gacgttggtg atatgccagg
     3961 tgacatggtc gcccttcttg atctctaccc gctcgggggt gaagtggctg cggatggccg
     4021 tcatgtagac cgtgacgttg ttgccgtcgc gctcgacacg ctcggtgcct tgctggggcg
     4081 catcagcccg cactgactgc gtatgcggat cccagccgat ttcgggatag acatcccacg
     4141 gcttgatctt gtcggccttg ataatctggg cgtagtgcgg ctcgccgtcg ggcaagggca
     4201 tgtcatagac cagcggcatg ttcgtgcccg gctggctgat atccaccaac tggaagtttt
     4261 gcggcagcag cgggccgacc ggcatgaagc gatcgacggc ccatttgttc atggccacca
     4321 gccagttgcc gtcgggagag acggtgtcgc cttcggccgt caccaggtga ccgatgttgt
     4381 aatgcaccgg cgtcttggtg acgagcgtcc acggcgggtc ttcgttcagt tcatcgaacg
     4441 aaccgcctag cgaccagcga gccacggccg aatcgaggaa caggctggtg taggcatagc
     4501 ccttgttgtc gaactgcgta tgcagcgggc caaggcccag ctcaacctgg gtctccataa
     4561 cggagtcgaa gtccagaatc ggcacgccat actggtcggt gtcgctcgtg ccggcctcga
     4621 tggccgccat gatcttgtcg aaaccgaaga cggtcacatg tgggtccagc ttgccggcga
     4681 cgatgacgaa ctcgccgtcg ggagtcacgt cggagccgtg ggggcttttc ggctcgggag
     4741 cgaagtagag cagtccttcg ccgatggccg tgtcgaggct gatgaccggg aagtcgttga
     4801 tcaggtcgaa cttgccggcc gcgtagactt cggccgcctt cttcaggttg atgatatgga
     4861 ggaagtccat atcgttctgg ctggcgccgg actcgaaggg cgggttgccc aattcgacgc
     4921 cgccggtggc cagttcggta ttgatggagt tgcagaagac ccaaccgtcg ctgaccagct
     4981 tgccgctgtc acacaggtct tgccagtagg gtggcagctc gatggcgaac gactggtcgg
     5041 tggcgatgcg gccggcctcg cggttgaact tccagaaggt gatcatgccc tggtaatctt
     5101 cttcatagtt ctcgatggag ctatatttga agcccagggg catgccatac tggccaccct
     5161 cgatgaccca gtcagtgttc ggggtcacaa aggtgccgcc gtggtcgctg agggtaatag
     5221 ggttcttgac gatctgcttg acttcgaagt cgcgcaggtc gatgaccgcc acgcgggcgt
     5281 tggccttgtc gttgatgaag agatattggc cgtcatattc gccgttggtc tcgctaaggc
     5341 cgggatgatg ggtatcggcc catgtcagcg gggtaccgtc ggccgccagg ccgccctctc
     5401 tcaacacctg cagcgtcccc agttcaccgt agccgaagcc ctgccagggt tcgggggtga
     5461 agacggcgat ctgcttgatg atgcgcatgg agggcagacc gatgacgtac acctggcccg
     5521 actggccgcc cgatgcgaac aaataatatt cgtcatgctt gccgctgggc gtataggtct
     5581 tcacggcatt gacgatgtcg cccggggtca ggccacgagc ttcggcgata acgttgatat
     5641 cttccgtcgc cccggcccct tgagggacgg ctgcggcgcc accgttgttg tcgcggcaac
     5701 tgacgatcag gacggccatg gccgccacga tcatcaatac cgtaatcaac ataatcggtc
     5761 tggtcgattt gcggttgagt gtcattttct tccgtttctc ctgtcttcgc atgccataaa
     5821 ggcacgccac aaataaataa tgggtgcaat gtcgacagga tagttgaaat gggtgctccg
     5881 gtctttgcat tattgcaaag accggcactg ttattgggta aatggagtga gtaggggagt
     5941 gagaaaacga aaaacaggcg gagatgtcgt ttaatctgtt ttcggtgctc gactgccggg
     6001 ctgagggatg tcttcggcca tgataaccag ttcgtgagca ttaactaacg ttacttgttt
     6061 tcggccggtc gtgataagcc cgctgctttc ccatttgctg aggatgcggc tgacattata
     6121 caagttggtg cccgtcatct gagccaggtc ttcccgactg agagatatat cgatcaagac
     6181 accttcttcc gtccgccggc cgaattggcg caccaggcgc agcagtgcgc gcgccacacg
     6241 ccgttcgacg cgttgggtgg ccagctcctg aaaccggtgc tgcatctggg tgaagcgttg
     6301 gctgatcata ttcatgccgt tgatggcgag ttgggcgttt tccttcatca ggtcgagcat
     6361 cgtttcccga ccccacccgg cggcagtgca gggttcgatt gcttcggcag tgagcgggta
     6421 tggcttcttg ttcagagcca tagcgatgcc caacccctca cccgagccaa agtaatcgat
     6481 gatgacctga tctccttcgc ttgtcacttg agaaagtttg acccggccgg aaaggatgac
     6541 gtagagcatc gtcgagtctt ccccttggtg gaagaaaaaa tcgccaggct cgacttcgta
     6601 gattcggcca tgagccataa tatgctcaca atcatctgag gagataccct taaatagggt
     6661 gcaaccagcc ataatttggc atggggttga cggtgaattc atagacctta atcataccat
     6721 atgaatcact atcgaggcct aatagtcatg gggttcgatg gcttaacgtc agccatatta
     6781 attcgggtct gtattgacgc tcttgtcgtg ggatattatt atttgagcat atctgacccc
     6841 tgtcggccgc ctgtatgtgg gttggggcag gcggccaaaa ttgcaaggag agaattttca
     6901 tgaagaaccg cttcagccgt ccgtatctga tcatgactgc tgcattgctc gcgctttttg
     6961 ttctggtact tggcgggcaa cgttctgccc aggcccagga tgcccaatgg cttggtgaat
     7021 tctggaacaa tcgggatctg aatggccccg ttgtcctgac ccgctgggag aacagcatcg
     7081 atttcaattg gttcgatggt tctcccgacc cgcgaatcaa tagtgacaat ttctcggctc
     7141 gctggacgcg caccgtcaac ttcccggccg gcacctatcg gttctacgcc acgatggatg
     7201 acggcatgcg catctggatc gacaatgttc tggtgattga ttcctggacc catagccagg
     7261 agcataccat gaccgttgat agattcatga atggcaatca ctcgatccgg atcgattatt
     7321 tcgaagcggg tggcgctgcc acggctcgct tcaattggcg actgatcaac aatgcaggcg
     7381 gcggccagtt cttcccaaac tggagagcgg agtatttcaa taatatgacg ttgtccggct
     7441 ccccttcatt gattcgtgat gaggcgacga tctccatcga ctggggtgat ggctcgcccg
     7501 gtccgggggt taattctgat ttttggtctg cccggtttac gcgacagttt actgcccgcc
     7561 ccggccaata tcgcattatc ctgaccagcg acgatggttc gcgcctgttc atcaacaatc
     7621 agttgatcat cgacaactgg cgtgcccagg ccgctaccag cgtgtcgacc gactttttca
     7681 ccaatggcgg cacgatggac gtgcgagttg agttcttcga tcgcaccggc ggggcgttgc
     7741 ttcgggccga tctgatcacg atcagtggcg gccttatcac ggggcagccg acacctccga
     7801 ccggcgaagg gggtgtttgc gccacggcac ccgccgggat gcaagggcaa tccaccgcga
     7861 ccactgtgtt gaatattcgg cgtggcccga gcacccagtt tgagacgatg ggcaccttgt
     7921 cccgatgcca gatcgtcccg ctttcgggct tccgcaatgc cgacagcacg tggatcca
//
LOCUS       DAOMQG010000129         7906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3944_length_7906_cov_7.108521, whole genome shotgun sequence.
ACCESSION   DAOMQG010000129 DAOMQG010000000
VERSION     DAOMQG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3944_length_7906_cov_7.108521"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLC06_08590"
     CDS             <1..253
                     /locus_tag="PLC06_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRO90541.1"
                     /translation="HVGNTLDGFGGCNTIGGTYSQFGANGLAISAGTRSLILCEDDVM
                     AQQEAFLNGLHTTAAFTIEGNTLIMRDGSGIETLRFQRQ"
     gene            617..2659
                     /locus_tag="PLC06_08595"
     CDS             617..2659
                     /locus_tag="PLC06_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRO90542.1"
                     /translation="MKRLVIIIGLILLLALAACDTMGPTTQELVPTEGVAVATQAPPL
                     TEDSSTVEQPQGETAAPGADLQGVTWEWVSLIDPMGQTTSSDPTRYTITFNPDGMAPV
                     LADCNTVISSYITDGLNLEILPGPATLMACPEDTQDQLFTASLAAADSYIVQDGELFI
                     TIDGGTGTLIFRSAPLAGDSGTQPSGPSLTGVTWEWIEATTSLGTTPVVDPTRYTITF
                     NDDGTANIKADCNNVLATYTSDGAGALTLALGPSTLVACPPDSQVDMFTAGLGVAGTY
                     SFADGDLLLINPAADGGVLRFRAAITPAGTASPDAELTDIVWEWISTTTPAEEIAAAD
                     PTRYRITFNTDGTANLTADCNVGNAAYTVGEDGSMSITLGVSTLAFCENSQDTIFREG
                     LAAAATYALDGGDLVINLANDGGAMRFRNGGAAEGMAEPPATGNLTGTTWEWVKTVTP
                     MEEIAATDQTRYNIVFNEDGSATIKADCNTGMATYTVDEGGAITITPGGMTLMMCPDD
                     SQATRFMADLTGAAIYFFQDGNLFIDLFASSGTMQFAPATVSAGGPVKGGGSVSGGTP
                     ANLTGTVWQMTLIQTPGGNLTINDPARYAVTFNADGSANIKADCNSVIAAYTIGEGNT
                     LTIMPGPSTMAYCGPGSFDQIFVGGLTNAMGYRLEDGNLLIDMLYESGSLVFSPAN"
     gene            2764..3183
                     /locus_tag="PLC06_08600"
     CDS             2764..3183
                     /locus_tag="PLC06_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90543.1"
                     /translation="MENQRPAGITFLAVAFILLGLLSLFWSLLVFGVGAATGITGAIF
                     GADSMRAFGSSNFWGGIIGVVGAVLDLIIAYGLLGLKKWAWMLALIGVAVNVITGITG
                     LMSGGIFALCCGLLGLVVPGIILYYLMRPDVRAAFGR"
     gene            3334..4212
                     /locus_tag="PLC06_08605"
     CDS             3334..4212
                     /locus_tag="PLC06_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRO90544.1"
                     /translation="MGKKDKGKEKKRAKTPPSVRDLLRVGPEFDLGAQDPRRVVAGPG
                     DKETARAAALALEPEITALQERLFAESKGGGKRRLLVVLQGMDTSGKGGAVKAIDRVC
                     EPIGLRVAGFGAPTKEELRHHYLWRINKALPPAGSIGIFDRSHYEDVLIVRVRNLVPE
                     DVWSGRYDEINDWEKKLADSGLVFIKCMLHISKAEQQARLLARLEEPDKRWKYNPGDV
                     DERELWDKYQEAYQVALTRCSTDYAPWYVIPADRKWHRDWLLTQLVAETLREMDPQYP
                     AVGYDVETEIGRVQGS"
     gene            4398..4670
                     /locus_tag="PLC06_08610"
     CDS             4398..4670
                     /locus_tag="PLC06_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90545.1"
                     /translation="MCCILSLLIFIGPRVAAFGWYIIDPARWSAAFSNILWPILGILI
                     VPWTTMAWVWMSPGGISGLEWAVVGMGLLVDLGAFGGGGYSRRHRD"
     gene            4685..5071
                     /locus_tag="PLC06_08615"
     CDS             4685..5071
                     /locus_tag="PLC06_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HRO90546.1"
                     /translation="MAIPKIETYRGLSMPRALLHLEGAAVFAAATGFYFLLGLSWWVY
                     LLFLFAPDLSAIGYLFGPRTGSVLYNLAHTIIWPLIIGLAGWWLGWSWSAPVALIWLA
                     HIGLDRLLGYGLKYPDAFKHTHLDQV"
     gene            5175..5390
                     /locus_tag="PLC06_08620"
     CDS             5175..5390
                     /locus_tag="PLC06_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90547.1"
                     /translation="MTLMTVSLEEEKVRGLLKEILLELIEDRPEIFHDIVLEAVEDAG
                     LLAAILEEESSELVDEAEIEAILQGSR"
     gene            5387..5653
                     /locus_tag="PLC06_08625"
     CDS             5387..5653
                     /locus_tag="PLC06_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRO90548.1"
                     /translation="MKTEFKSSFARDLRKLNDAATLARVQHLITLVKQTGSLDQIPGV
                     KKLQSSGNHYRIRLGDYRVGIIVSGETVTFVRFLHRRDIYRYFP"
     gene            5670..6434
                     /gene="surE"
                     /locus_tag="PLC06_08630"
     CDS             5670..6434
                     /gene="surE"
                     /locus_tag="PLC06_08630"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRO90549.1"
                     /translation="MHILVTNDDGVLAPGLLALARAMKPFGDVTVLAPDRNWSGGGHV
                     KTLHRPLRVRETTLADGTPALMSDGAPSDCVALALLGRVPKVDLVVSGINSFGNLGHD
                     VTYSGTVTAAMEAVIAGVPGFAFSLAATDETAERDYGPAADYAGRIVGKMIARGLSPD
                     LLLNINVPCLPAAQIRGMRVTRQGLRVYRDRLDERIDPRGHPYYWIGGEYPTGVPDEG
                     TDFGALAAGYVSITPLRLDLTNSMVMEQLAGVTSDE"
     gene            complement(6494..>7906)
                     /locus_tag="PLC06_08635"
     CDS             complement(6494..>7906)
                     /locus_tag="PLC06_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO90550.1"
                     /translation="VSMASTRPYQRLLYGVGSIGVALSYQAFSTYIQFLYIDTLGVRA
                     AWIGLVWAVYGIWNSINDPLAGYLSDRTRTRWGRRLPWMYGALLPLVITFYLLWFPFK
                     GVNWSEFSLLFYFLAIVLAFDLFWTVFVMNWTSLFPEMVSSESDRTGLSALRESFSIV
                     GLLVGVALPPLIAGADWSNRGQMAYLLTAVTLISLLLTLFGSKERREFAAEPSPPFRE
                     SLRLTLGNRDFLFFLAANLMIQYVFLGLAAAIPFYAKYVLKIQGPTTLGGVVLDAEMQ
                     NSLLLAAAFLAALPAMVVWLKLSQRFGAWKTLRAASLVGAAIALYFFFPTTFVGGLLG
                     TILFGLVLAALLMLTNPLLADITDADEVAHGARREGMFFGMNGLVIRFAFVIQGILTA
                     IVFSLTGYVSPSAGVLNPIQPESALFGMRLLTGGTTAVALVAAFLLLGGYSLHGRRAE
                     KIRAEAAAMQEEKKLGMGEN"
BASE COUNT         1496 a         2473 c         2487 g         1450 t
ORIGIN      
        1 ccacgtgggc aacaccctcg acggcttcgg cggctgcaac accatcggcg gcacctattc
       61 gcagttcggc gccaacggtc tggccatcag cgccggcacg cgcagcctga tcctttgcga
      121 ggacgacgtg atggcgcagc aggaagcctt cctgaatgga ctgcacacga cggccgcctt
      181 cacgatcgag ggcaacacgc tcatcatgcg cgacggcagc ggcatcgaga ccctgcgctt
      241 ccagcggcaa taacgatcgc cccccgatag aaaccgggtt tcttccggga aacccggttt
      301 cttcgaaata cgaagtacga agtacgaaag acgcgctctt ccttcgtact tcgtctttcg
      361 tacttcaccg acgctcgtca ctcatcactc gtcactctct tgcggcggtc ggcagtcgtc
      421 cggccgcggt ctgtcgtctg tcgtcgcgcg acaggagtcg tgcgacagga gtcgcgctcc
      481 cattgcgaaa cccgacaaga taatgtatta tgtttagcta gaggccccgg ccgtttcggt
      541 ggcgggcgcg cgcctgtttc cgaggtcggc tcgcgggatt gtctgtccca agtagtacat
      601 agagggagaa aggaaaatga aacgtcttgt cattatcatt ggcctgattc tgctgttggc
      661 cctggcggca tgcgacacga tgggcccaac cacgcaggaa ctggtgccga ccgaaggggt
      721 ggcggtggcc acccaggcac cgccgctgac cgaggactcg tcaaccgtcg agcagccgca
      781 aggggagacg gccgcgcccg gcgccgacct gcaaggcgtg acctgggaat gggtgtccct
      841 gatcgaccca atggggcaaa cgacatccag cgaccccacc cgctacacga tcaccttcaa
      901 ccctgacggc atggctcccg tgctggccga ttgcaacacc gtcatcagca gctacatcac
      961 cgacgggttg aaccttgaga tccttcccgg cccggccacc ctgatggcct gccccgagga
     1021 cacccaggat caactgttca ccgccagcct ggccgcggcc gacagttata tcgtccagga
     1081 cggcgaactg ttcatcacca tcgacggcgg cacgggcaca ctgattttcc gctcggcccc
     1141 gctggccggc gacagcggca cgcagccgtc ggggccgtcc ctgaccggcg tcacctggga
     1201 gtggatcgag gcgacgacct ctctgggcac aaccccggtg gtcgacccca cccgctacac
     1261 catcaccttc aacgacgacg gcacggccaa catcaaggcc gactgcaaca atgtgctggc
     1321 cacctacacc agcgacggag ccggcgcgct gacgctggcc ctcggcccct cgacgctggt
     1381 cgcctgcccg cccgattcgc aggttgacat gttcaccgcc gggctgggcg tcgccggcac
     1441 ctactccttc gccgatggcg acctgctgct catcaacccg gcggccgacg gcggcgtgtt
     1501 gcgcttccgg gcggccatta cccccgccgg aaccgcctcg cccgacgcgg agctgaccga
     1561 catcgtctgg gagtggatat ccaccaccac cccggccgag gagatcgccg cggccgaccc
     1621 gacgcgctac cggatcacct tcaataccga cggcacggcc aacctgacgg ccgactgcaa
     1681 cgtgggtaac gcggcctaca ccgtcggcga ggacggctcc atgtccatca cgctcggcgt
     1741 gtccacgctg gcgttctgcg agaactcgca ggacacgatc ttccgcgaag ggctggccgc
     1801 cgcggcgact tatgccctgg acgggggcga tctggtcatc aacctggcca acgacggcgg
     1861 cgcgatgcgc ttccgcaacg gcggcgcggc cgaaggaatg gccgaacccc cggcgaccgg
     1921 caatctgacc ggcacgacct gggaatgggt gaagacggtg accccgatgg aagagatcgc
     1981 ggccaccgac cagacccgct acaacatcgt cttcaacgag gacggctcgg cgaccatcaa
     2041 ggccgactgc aacaccggca tggcgaccta cacggtcgat gagggtggag cgatcaccat
     2101 cacccccggc ggcatgacgc tgatgatgtg ccccgacgat tcgcaggcga cgcgcttcat
     2161 ggccgacctg accggcgcgg cgatctactt cttccaggac ggcaatctgt tcatcgacct
     2221 gttcgccagc agcggcacca tgcagttcgc cccggcgacg gtgagcgcgg gcggcccggt
     2281 gaagggcggt ggcagtgtgt ccggcggcac gcccgctaat ttgacgggca cagtctggca
     2341 gatgacgctg atccagacgc ccggcggcaa cctcaccatc aacgacccgg cgcgctatgc
     2401 cgtcaccttc aacgccgacg gctcggccaa catcaaagcc gattgcaatt ccgtcatcgc
     2461 cgcctatact atcggcgagg gcaacacgct gaccatcatg ccggggccgt cgaccatggc
     2521 ctactgcggg ccggggtcgt tcgaccagat cttcgtgggc ggcctgacca acgcgatggg
     2581 ctatcggctg gaagacggca atttgcttat cgacatgctc tatgagagcg gttcactggt
     2641 attctcgccg gccaattaag gcccggcgta aattaaaacc tgacaggttg gcgatgaacg
     2701 gttttgtcca atcaaggcct ctatcgccaa cctgtcaggg ttctacttca ataggagaaa
     2761 ctcgtggaaa atcaacgtcc ggcgggaatt acatttttgg cggtcgcttt tatccttctc
     2821 ggtttgctat ccttgttctg gagcttgctg gtctttggcg tgggggcggc caccggcatc
     2881 accggggcca tcttcggcgc ggacagcatg cgcgcgttcg gctcgtcgaa cttctggggc
     2941 ggcatcatcg gcgtggtcgg ggccgtgctc gatctgatca tcgcctacgg cttgctgggc
     3001 ctgaagaagt gggcctggat gctggcgctg atcggtgtgg ccgttaatgt catcaccggc
     3061 atcaccgggt tgatgagcgg gggaatcttc gccctttgtt gcggcttgct gggcctggtc
     3121 gtccctggca tcattctcta ttacctgatg cgccccgacg tccgcgcggc gttcggtcgc
     3181 taggaaccct ggcgtaatcc gccggatggc gttctgcggc cgccatccgg tggattgcgc
     3241 tgatgtagcg caattcgctg aattgcgtct ttgatcgcaa tccggcggat tgcgccacag
     3301 ccggcggaat gggctacagt attggagatc aacatgggca agaaagacaa aggcaaagag
     3361 aaaaagcgcg cgaaaacgcc cccttccgtt cgcgacctgc tgcgcgtggg gccggagttc
     3421 gatctgggcg cgcaggaccc gcgacgggtt gtggccgggc cgggcgacaa ggagacggcg
     3481 cgggccgcgg cgctggcgct ggaacccgaa atcaccgcgc tgcaggagcg cctcttcgcc
     3541 gagtcgaagg ggggaggcaa gcggcggctg ctggtcgtcc tgcagggcat ggacacgtcc
     3601 ggcaagggtg gggcggtcaa ggctatcgat cgcgtctgcg agccgattgg cctgcgcgtt
     3661 gccggcttcg gcgcgcccac caaagaggaa ctgcgccatc attatttgtg gcgcatcaat
     3721 aaggcgctgc cgccggccgg ctccatcggc atcttcgacc gctcccatta cgaagacgtg
     3781 ctcatcgttc gcgtgcgcaa cctcgttcct gaagatgtgt ggagcggccg ctacgacgag
     3841 atcaacgact gggaaaagaa gctcgccgac agcgggctgg tgttcatcaa gtgcatgctc
     3901 cacatctcca aagccgagca gcaggcgcgc ctgctggccc ggctggagga gccggacaag
     3961 cgctggaagt acaaccccgg cgacgtggac gagcgggaac tgtgggacaa gtatcaggag
     4021 gcctatcagg tggcgctgac ccgttgctcc accgactacg cgccgtggta cgtgatcccc
     4081 gccgaccgca agtggcaccg cgactggctg ctgacccagc tggtggccga aaccctgcgc
     4141 gagatggacc cgcaataccc ggcggttggt tatgatgtgg agacggagat tgggcgggtt
     4201 cagggtagtt gatcagtgtg cggttgatca gtattcagta ggtcagtaga tcagtattca
     4261 gttcactgac cactggccac tgaccacgga cgacagacca cgggaagcgc ctctttcgta
     4321 cttcaagatg aagagcgtgc tcttgccgcc gtcgtcggtc gtccgtcgtc cgtcgtcttt
     4381 cataaagcga ggcaaacatg tgctgcatcc tctcattact catcttcatc gggccgcgag
     4441 ttgccgcctt tggctggtat atcatcgacc cggcgcgctg gagcgcggcc ttcagcaata
     4501 tcctctggcc gattttgggc atcctgattg taccctggac gacgatggcc tgggtctgga
     4561 tgtcgcccgg cggcatatcg ggcctggagt gggccgtcgt cggcatgggg ctgctggtcg
     4621 atctgggcgc gttcggcggt ggcggctata gccgtcgcca tcgcgactag cggccggcct
     4681 gcttatggcc atacccaaaa ttgagactta tcgcgggttg tccatgccgc gcgcgttgct
     4741 ccatctggaa ggcgcagccg tgttcgccgc cgccaccggc ttctatttcc tgctgggctt
     4801 gtcgtggtgg gtctacctgc tcttcctctt tgcgcctgat ctatcagcaa tcggctacct
     4861 cttcggcccg cgaaccggca gcgttctcta taatctggcc cataccatca tctggccgct
     4921 gatcatcggc ctggccggct ggtggctggg ctggtcgtgg tcggcacccg tcgccttaat
     4981 ctggctggcc catattgggc tggatcggct gctggggtat ggcctgaagt atcccgacgc
     5041 attcaaacat acgcatctcg atcaggtatg accccgcgca agctcctgaa gcgtgttcgc
     5101 ggctctgtcc cgcctgtggg cctgtgggcc tgtgtgctat aattgagcta tccggacata
     5161 cggagagcga tctgatgacg ttgatgaccg tttcccttga agaagaaaaa gtgcgtggac
     5221 tgctcaaaga gatccttttg gaactcatcg aagatcgccc ggagatcttc cacgatatcg
     5281 tcctcgaagc cgtcgaggac gccggtctgc tggcagcgat ccttgaagag gagtcgagcg
     5341 agttggtgga tgaggccgag atagaggcga tcttgcaggg tagtcgttga aaaccgagtt
     5401 taaatccagc tttgcccgcg atctgagaaa actgaacgat gccgctacct tggcgcgcgt
     5461 tcaacacctg atcacgcttg tgaaacagac aggtagcctg gaccaaatcc ccggcgtcaa
     5521 gaagcttcag agttcgggaa accattatcg gattcgactg ggcgactatc gtgtgggaat
     5581 tattgtgtca ggcgaaaccg taacatttgt tcgttttcta catcgccggg atatctatcg
     5641 ttacttccca taatatcccc tatattttta tgcatattct tgttaccaac gatgatggcg
     5701 ttcttgcgcc ggggctattg gcgctggcgc gggcgatgaa accttttggc gatgtgacgg
     5761 tgctggctcc cgatcgcaac tggtcgggcg gggggcatgt gaagacgctc catcggccgc
     5821 tgcgcgtgcg cgagacgacg ctggccgacg gcactccggc cctgatgagc gacggcgcgc
     5881 cgtcggattg cgtggcgctg gcgctgctcg gccgcgtgcc gaaggtcgat ctggtcgtgt
     5941 cgggcatcaa ctccttcggc aatctgggcc acgatgtcac ctacagcggc accgtgacgg
     6001 cggcgatgga agcggtgatc gccggcgtgc ccggcttcgc cttctcattg gcggccaccg
     6061 acgaaacggc cgagcgcgat tatggcccgg cggccgatta tgccgggcgc atcgtgggga
     6121 agatgatcgc gcgcggcttg tcgcccgacc tgctgctcaa catcaacgtg ccctgccttc
     6181 cggccgccca aattcggggg atgcgcgtca cgcggcaggg gttgcgcgtc tatcgtgacc
     6241 gtctcgacga gcgcatcgac ccacgcggcc atccctacta ctggatcggc ggcgaatacc
     6301 ccaccggcgt gcctgacgaa ggcaccgact tcggcgcgct ggcggccggc tatgtgtcga
     6361 tcaccccgct gcgtctcgac ctcaccaaca gcatggtgat ggaacaactc gcgggagtga
     6421 cgagcgacga gtgacggtga attacgaatt acgaattacg aaagaggttc tcttcattcg
     6481 taattcctga ttcctaattc tcccccattc ccaacttctt ctcttcctgc atcgccgccg
     6541 cctcggctct gatcttttcc gcgcggcggc cgtggagcga atagccgccc aggagcagga
     6601 acgccgccac cagcgcgacg gccgtcgtgc cgccggtcag caggcgcatg ccgaacagcg
     6661 ccgattcggg ctggatgggg ttgagcacac ccgcgctggg gctgacgtag ccggtcaggc
     6721 tgaagacgat ggccgtcagg ataccctgga tgacgaaggc gaagcggatg acgaggccgt
     6781 tcatgccgaa gaacatgccc tcgcgccgcg cgccatgggc cacttcgtcg gcgtcggtga
     6841 tgtcggcgag gagggggttg gtgagcatca acagggcggc cagcacgagg ccgaagagga
     6901 tcgtgccgag caatcccccg acgaaggtgg tgggaaagaa gaagtagagg gcgatggccg
     6961 cgcccaccag cgacgctgcc cgcagcgtct tccaggcccc aaagcgctgc gacagcttca
     7021 gccagacgac catggccggc agcgcggcca ggaaggcagc cgccagcagc agcgagttct
     7081 gcatctccgc gtcgagcacc accccgccca gcgtcgtcgg gccttgtatt ttcagcacat
     7141 atttggcgta gaacgggatg gccgccgcta gccccaggaa aacgtactgg atcatcaggt
     7201 tggcggcaag gaagaagagg aagtcgcgat tgcccaacgt caggcgcaat gattcacgga
     7261 acggcggcga cggctcggcg gcgaactcgc ggcgctcctt gctgccaaac agcgtgagca
     7321 gcaaagagat gagcgtcaca gccgttaata aataagccat ctggccgcgg ttgctccagt
     7381 ccgccccggc gatcagcggt ggcagggcca caccgaccag caggccgacg atggagaacg
     7441 attcgcgcag cgccgacagc cccgtccggt ccgattcgct gctgaccatc tccgggaaga
     7501 gcgacgtcca gttcatcacg aacacagtcc agaacaggtc gaacgccagc acgatggcca
     7561 gaaaatagaa cagcagcgaa aactccgacc agttcacacc cttaaagggg aaccacagca
     7621 agtagaaggt gatgaccagc ggcagcagcg cgccatacat ccacggcagg cggcggcccc
     7681 agcgggtgcg cgtccgatcg ctcagatagc ccgccagggg atcgttaatc gagttccaga
     7741 tgccgtagac tgcccagacc aggccgatcc acgccgcgcg cacgccgagc gtgtcgatat
     7801 agaggaactg gatgtaggtg gagaaagcct gataggagag ggcgacgccg atggagccga
     7861 cgccgtagag caatcgttga taggggcggg ttgatgccat ggatac
//
LOCUS       DAOMQG010000130         7862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3969_length_7862_cov_6.306904, whole genome shotgun sequence.
ACCESSION   DAOMQG010000130 DAOMQG010000000
VERSION     DAOMQG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_7862_cov_6.306904"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLC06_08640"
     CDS             <1..323
                     /locus_tag="PLC06_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90551.1"
                     /translation="REGQFAAILADIPDDATHVIVGGDFNTITGRGVNALAGQFAAAG
                     LDHASAELGPTFTRLGLRPSATDHIFSRGFERLDAGVLGEVRASDHFPVWVRLGWPPG
                     SSTN"
     gene            complement(342..1361)
                     /locus_tag="PLC06_08645"
     CDS             complement(342..1361)
                     /locus_tag="PLC06_08645"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRO90552.1"
                     /translation="MATIWMVRAGRGGVYADEFLNNNFVAIGWNIGASSLTASKSELE
                     NLYRRTYPESSDGQVKNAIGQIDAFLHRFAINDQILTFDREKRRYLLGVIRSTPEYLP
                     DNIEDLCTIRDVEWTSKIFKDRLKLETRNSLGAIQTVFQIKGDAAADVIANAVSLEHD
                     ESDIRAKPTHVEVERAKVIEEQARAELLEKAEESIEDRIVKLNWEEMQELVAGLLRAM
                     GYKTDVSPRGSDRGVDIFASPDGLGLEEPRIFVEVKHRPTQSMGAQDVRSFLGGRVDS
                     DRCLYVSTGGFTKDARYEADRAKTGIRLLTLVDLRRLLVENYEKLDEEIRALVPLKRV
                     YVLAA"
     gene            complement(1454..1783)
                     /locus_tag="PLC06_08650"
     CDS             complement(1454..1783)
                     /locus_tag="PLC06_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007312575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRO90553.1"
                     /translation="MMEYKGYIVRVEIDNDAGILHGEVINTRDVITFEGTSIDELRQA
                     LADSVEDYLAFCAERGEEPDKPFSGKFSLRVDPELHRQITVKAKLADKSVNSWITETL
                     EAAVGEY"
     gene            complement(1801..2235)
                     /locus_tag="PLC06_08655"
     CDS             complement(1801..2235)
                     /locus_tag="PLC06_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO90554.1"
                     /translation="MNLYLDTSALVKLYIREQGTDEVNQWMTQADFKATSLITLAEAS
                     AALSRTLRMKIISQGTGEEAIRLLRKQWPLYLKTPMTEKTVARAAELAWDLGLRGYDA
                     VHLASAELWQAALGLPVALATYDSQLAAGAGQLGMDVLPTNE"
     gene            complement(2232..2507)
                     /locus_tag="PLC06_08660"
     CDS             complement(2232..2507)
                     /locus_tag="PLC06_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRO90555.1"
                     /translation="MTQLTVGIRDLKAQLSEYLRQVKAGETVIITEHGRPIGRIVPEP
                     ESPEERIQRLLDSGELLWNGEHLPPFEPVGENTGSLLISDVVSELRE"
     gene            complement(2612..4789)
                     /gene="scpA"
                     /locus_tag="PLC06_08665"
     CDS             complement(2612..4789)
                     /gene="scpA"
                     /locus_tag="PLC06_08665"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRO90556.1"
                     /translation="MTTPDFTQIPYQSDYPRQSLADWQAALERETGRPAAEFLWHTME
                     GIDVRPLYTASDTADLPNFDHAAGLPPFLRGPYPAMYVARPWTVRQYAGFSTAEESNA
                     FYRRNLAGGQRGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAISSLRDMQTLFDGIPL
                     DKMSVSMTMNGAVLPIMAFYIVAAEEQGVSHEQLTGTIQNDILKEYMVRNTYIYPPEG
                     SMRIVGDIFSYTAREMPKFNSISISGYHMQEAGATADIELGYTLADGLEYVRTGLRAG
                     LAIDDFAPRLSFFWGIGMNYFMEIAKMRAARLLWAKLIKQFDPQDPKSMALRTHSQTS
                     GWSLTEQDPFNNVARTCIEAMAAVLGGTQSLHTNALDEAIALPTDFSARIARNTQLYL
                     QHETGITQIVDPWGGSYLVETLTNELARRASAHIREIDELGGMAKALETGLPKMRIEE
                     AAARRQARIDSKAETIVGVNKYRPASEAPIDILEVDNTAVREQQIARLAEVRAGRDDD
                     AVRHALHALTHAAAVGEGNLLELAVDAARARATLGEISDALERVWGRHKAVVRSVGGV
                     YRAEYGDDEEIAHARKLADDFAAVEGRRPRILVAKMGQDGHDRGAKVIATAFADLGFD
                     VDIGPLFQTPEEVARQAMENDVHVVGVSSLAAGHKTLVPQLAAELDRLGRADIMIVVG
                     GVIPAQDYKFLHANGASAVFGPGTVIPVAAGKIVEELGRRLGH"
     gene            complement(4871..7033)
                     /locus_tag="PLC06_08670"
     CDS             complement(4871..7033)
                     /locus_tag="PLC06_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRO90557.1"
                     /translation="MDDSRRNDPMLSFDEFPEPLYDEWQQAAVDSLKGRPFDKLITPT
                     WEGIPLQPLYGREDTGDIAAAGTIPGASPYMRGSDAAGYLARPWAIAQELACPTPAAF
                     NRALRFDLEHGQTVANLLLDGPTRAGKDPDAAQPGEVGRGGVSLASVDDVAAALNGVD
                     LATVPITIRAGTAALPLLALLVAYARRAGRPASDLHGCLEDDPLGVLAHEGTIPLSLG
                     RAYDEMAQVTLWAERHAPRLQTVAVHTYPYHNAGANAVEELAFALATGVAYLRAMQRR
                     GVDVDAAAGHMRFDFAVGGNFFMEIAKLRAARLLWSQAVAAFGGDDEAQKMRPPLMRL
                     HARTARRNKTITDPYVNMLRVTTEALAAAVGGVESLHVAPYDEPARPPDEFSRRVARN
                     AQVILQEEAHLTAVIDPAGGSYAVEVLTDRLARDAWALFQEIEGVGGMAEALKDGFAQ
                     TRVAAAADRRATHLATRRDVLVGTNQFANPKEPAPLADETDYAAIHRERATQMTQLRT
                     QDDDPTAHVAAIERLREMVAAPPETMVETAVAAAMAGATLGELTRTLRLNDGARPIIT
                     PVSLSRAAQPFEALRIQALDYAKKHGRPPRVFLANLGPPRQHKARADFAQGFFEVGGF
                     EIITNNGFPDAEAAAAAALASGAPAVVICSTDETYPELVPPLVKAIRQKTRETVIILA
                     GRPAEQLDALKHAGVDEFIYMGADAAALNGWLLEQIAN"
     gene            7342..7566
                     /locus_tag="PLC06_08675"
     CDS             7342..7566
                     /locus_tag="PLC06_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90558.1"
                     /translation="MPPFDEDAGQSGTGLASIEAQGATPGDERIEAMPGHARENLATP
                     FAITPTPPAPQVHDVVRRRPGPFPENYHVN"
     gene            7556..>7862
                     /locus_tag="PLC06_08680"
     CDS             7556..>7862
                     /locus_tag="PLC06_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO90559.1"
                     /translation="MSTDSIHAVTGAFGFSGKYITQRLLSEGCRVITLTNSADRANPF
                     GGRVAAYPFHFEQPELLAEALAGVETLYNTYWVRFNHPLFRHADAVRNTLILFEAARQ
                     "
BASE COUNT         1386 a         2424 c         2453 g         1599 t
ORIGIN      
        1 tgcgcgaggg gcagtttgcg gccatcctcg ccgacatccc cgacgacgcg acccacgtca
       61 tcgtcggcgg cgatttcaac accatcaccg ggcggggggt aaacgcgctg gccgggcagt
      121 tcgccgccgc cggactggac cacgcctcag ccgaactggg gccgacgttc acgcgcttgg
      181 ggctgcggcc gtcggcgaca gaccacatct tttcgcgggg gttcgagcga ctcgacgccg
      241 gagtgttggg cgaggttcgc gccagcgatc attttccggt gtgggtgagg ctgggatggc
      301 ccccaggcag ttcgaccaat taatcaagtt attcaagtta atcaagccgc cagtacatag
      361 acccgtttga gtggaaccag ggcgcgaatt tcctcatcca gcttctcgta attctcaacc
      421 aagagccgtc gaaggtcgac aagcgtaagc agacggatgc cggttttggc tcgatctgct
      481 tcatagcggg catctttcgt gaacccacct gtgctgacgt aaagacaacg atcgctatcg
      541 acacgtccgc cgagaaatga acgaacatcc tgagcgccca tgctttgagt cggccgatgt
      601 ttcacttcga cgaaaatccg tggttcttcc aggcctaacc catccggtga ggcgaaaata
      661 tcgacgccac gatccgagcc acgtggggag acatcggtct tatagcccat tgctcgcaac
      721 aatccggcta caagctcctg catttcttcc caatttagtt taacgattcg atcttcaatg
      781 gattcttccg ctttttccaa cagctcggca cgcgcttgtt cttcgatcac ctttgccctt
      841 tcaacctcaa catgtgtggg ctttgctctg atgtcagact catcatgttc cagggaaaca
      901 gcattcgcaa tgacatcggc tgccgcatct ccttttattt ggaatactgt ctgaattgct
      961 cccaaagaat tgcgcgtctc tagtttgagc cgatctttaa atatcttgga tgtccactct
     1021 acgtcccgaa ttgtacagag atcctcgata ttatcgggga gatattcagg ggtggaacgt
     1081 atgaccccta ataggtaacg ccttttctcg cggtcgaacg tcaatatttg atcgtttatg
     1141 gcgaacctgt gtagaaatgc gtctatctgc ccgatagcat tcttgacctg tccgtcggaa
     1201 ctctccggat aggtgcgtcg atagaggttt tccaactcgg atttagaggc agtaagacta
     1261 gaggctccaa tgttccagcc aattgctacg aaattattgt tcaagaactc gtcagcgtat
     1321 acaccgccgc gacctgcacg aaccatccag attgttgcca tatgttgtta agtctcgaag
     1381 ctaaatttcc aaatatggta gcgtctctaa cccgatatgc ctactcaacc gccaatagtc
     1441 gcaattgcta aatctaatat tccccaaccg ccgcttccag cgtctcggta atccaactat
     1501 tgacactttt atcagccaat ttggctttga ccgtaatttg gcgatgcagt tcgggatcaa
     1561 cacgcagact gaacttgccg gagaaaggct tatccggctc ttcaccccgt tcggcgcaaa
     1621 aggcgagata gtcttcaacc gaatcggcca acgcctgccg aagttcatca attgacgttc
     1681 cctcaaacgt aatcacgtcg cgcgtgttaa tgacttcgcc atggagaatc cccgcgtcgt
     1741 tatcgatctc aacccggacg atatagcctt tatactccat catggtttga tacctgaatt
     1801 ttattcattt gtcggtaaca catccatccc cagttgcccg gccccggccg ccaattgact
     1861 gtcatacgtc gccagagcaa ccggcaatcc cagtgccgcc tgccacaatt ctgccgaagc
     1921 aaggtggacg gcatcgtaac cgcgcaatcc taaatcccag gccagctcgg ccgcgcgagc
     1981 gacggtcttt tcagtcatcg gggttttaag gtaaagcggc cactgcttac gcaataggcg
     2041 gattgcctct tctcccgttc cttgcgatat gattttcatg cgcaacgtgc gggagagcgc
     2101 cgcgctggct tcggccaatg taatcaggct ggttgcctta aaatcagcct gagtcatcca
     2161 ctgatttacc tcatccgtcc cctgttcgcg aatgtacagc ttcaccaatg cgctggtatc
     2221 aagatacagg ttcattcgcg cagctcggat acgacgtcgg aaataagcag gctgccggta
     2281 ttttccccga ccggctcaaa aggcgggaga tgttcgccgt tccagagcag ttctccactg
     2341 tcgagcagcc tctgaatgcg ctcttcgggc gactcaggtt caggtacaat gcgcccgatt
     2401 ggtcggccat gttcggtgat gatcaccgtt tcaccggcct tcacctgccg cagatattcg
     2461 cttaattgcg ccttgagatc acgtatgccg actgttagtt gagtcatggc tgaattgcct
     2521 caaataatat gactacattg tacaccaatg tggtcatatt ggtcaatctt tagaaaccgg
     2581 gcttctgcca gaaacctggt ttctaaagcc attaatgccc caaccgcctc cccaactcct
     2641 ccacaatctt ccccgccgcc accgggatga ccgtgccggg gccgaagacg gccgatgcgc
     2701 cgttggcatg gaggaacttg tagtcctggg cggggatgac gccgccgacg acgatcatga
     2761 tgtcggcccg acccaggcgg tcgagttcgg ccgccagttg cggcacgagc gtcttgtggc
     2821 ccgcggccag cgagctgacg ccaaccacat gcacgtcgtt ctccatcgcc tgccgggcca
     2881 cctcttccgg cgtctggaac agcgggccga tgtcgacgtc gaagcccagg tcggcgaagg
     2941 cggtggcgat caccttggcc ccgcggtcgt gaccgtcctg gcccatcttg gccacgagga
     3001 tgcgcggccg ccgtccctcc actgcggcga agtcgtcggc cagcttgcgg gcgtgggcga
     3061 tctcctcgtc gtcaccatat tcggcgcgat agacgccgcc aaccgaacgt accacggcct
     3121 tgtgcctccc ccagacgcgc tccagcgcat ccgatatttc gcccagcgtg gcccgcgcgc
     3181 gggcggcatc cacggccagc tccagcaggt tgccctcgcc gacggcggcg gcatgggtca
     3241 gggcgtggag cgcgtgccgc acggcgtcgt cgtcgcgccc ggcgcgtacc tcggccagcc
     3301 gggcgatctg ctgctcgcgc acggcggtgt tgtccacttc caggatgtcg atcggcgctt
     3361 cgctggccgg gcgatatttg ttgacgccga cgatcgtttc cgctttcgag tcgatgcgcg
     3421 cctggcggcg ggcggccgct tcctcaatgc gcatcttggg caggcccgtc tccagcgctt
     3481 tggccatgcc gcccagctcg tcgatctcgc ggatgtgggc tgacgcacgg cgagccagct
     3541 cgttggtaag cgtttccacc agataagagc cgccccacgg atcgacgatt tgtgtgatac
     3601 ccgtctcgtg ctgcaaatag agctgtgtgt tgcgggcgat gcgggccgag aagtcggtcg
     3661 gcagggcgat ggcctcgtcg agggcgttgg tgtgcagcga ttgcgtgccc cccagcaccg
     3721 cggccatcgc ctcgatgcac gtccgggcca cgttgttgaa cgggtcctgc tcggtcaggc
     3781 tccagccgct ggtctggctg tgggtgcgca gggccatcga cttggggtct tgcgggtcga
     3841 actgcttgat gagtttggcc cacagcagcc gcgcggcgcg catcttggcg atctccatga
     3901 agtagttcat gccgatgccc cagaagaagg acaggcgcgg agcgaagtcg tcgatagcca
     3961 ggcccgcccg caagccggtg cggacgtatt ccagcccgtc ggccagcgtg tagccgagtt
     4021 cgatatcggc cgtggccccc gcttcctgca tgtggtagcc ggagatgctg atgctgttga
     4081 atttgggcat ctcgcgggcg gtgtagctga agatgtcgcc gacgatgcgc atcgaccctt
     4141 cgggcgggta gatgtaggtg ttgcgcacca tgtactcttt caggatgtcg ttctggatgg
     4201 tgccggtcag ttgctcgtgg ctcacgccct gttcctcggc ggcgacgata tagaaggcca
     4261 tgatcggcag caccgcgccg ttcatggtca tggagaccga catcttgtcc agcgggatgc
     4321 cgtcgaagag ggtttgcatg tcgcgcaggg agctgatggc cacgcccgcc ttgcccacgt
     4381 cgccgacgac gcgcgggtgg tcgctgtcgt agccgcgatg ggtggccagg tcgaaggcga
     4441 cggagaggcc gcgctgcccg ccggccaggt tgcggcgata gaaggcgttg ctctcctcgg
     4501 ccgtgctgaa accggcgtat tgtcgcacgg tccacggccg ggcgacgtac atggccgggt
     4561 aggggccgcg caggaagggc ggcagcccgg cggcatggtc gaagttgggc agatcagccg
     4621 tgtcgcttgc ggtatagagc ggccgaacat caatgccctc catcgtgtgc cacaggaact
     4681 cggccgccgg gcggccggtc tcgcgctcca gcgcggcttg ccagtcggcg agcgattgcc
     4741 gggggtagtc ggattggtag gggatttggg tgaagtcggg tgtggtcatg ggttggtttc
     4801 agtaggccag taggtcagtc gatcagtatt cagtggatcg gtaggtctgt cgttggtgtc
     4861 gtactgttca tcaattagcg atctgctcta gcagccatcc attcaatgcc gctgcatccg
     4921 cccccatata gataaactca tccacccccg catgcttgag cgcgtccagc tgctcggcgg
     4981 ggcggcccgc gaggatgatg actgtctcgc gggtcttctg gcggatggcc ttgaccagcg
     5041 gcgggacgag ttcggggtag gtctcgtcgg tggagcagat gacgacggcc ggcgcaccgg
     5101 aggctagcgc ggccgcggcg gccgcttcgg cgtcgggaaa gccgttgttg gtgatgatct
     5161 cgaatccgcc gacctcgaag aacccctggg cgaagtcggc gcgggccttg tgctggcgcg
     5221 gcgggccgag attggcgagg aagacgcgcg gcggccggcc gtgtttcttt gcatagtcca
     5281 atgcctgtat gcgcagcgct tcaaacggct gtgcggcgcg gctcagggag acaggggtga
     5341 tgatcggtcg cgctccatcg ttcaatcgca gcgttcgggt caactcgccc agcgtggccc
     5401 cggccatggc ggcggcgacg gccgtttcga ccatcgtctc cggcggcgcg gcgaccatct
     5461 ctctcaagcg ttcgatcgcg gccacgtggg ctgtcgggtc gtcgtcctgg gtgcgtagtt
     5521 gggtcatctg cgtcgcgcgc tcgcggtgga tggcggcgta atcggtctcg tcggcgagcg
     5581 gggccggttc cttggggttg gcgaactgat tagtccccac cagcacgtcg cggcgggtcg
     5641 ccagatgagt ggcccggcgg tcggccgcgg cggcgacgcg ggtctgggcg aagccgtcct
     5701 tcagcgcctc ggccatgccg cccacgccct cgatctcctg gaaaagcgcc caggcgtcgc
     5761 gggccagccg gtcggtgagc acctcgacgg cgtagctgcc gccggcgggg tcgataaccg
     5821 ccgtcagatg ggcctcttcc tgcaggatga cctgcgcgtt gcgggcgacg cggcgcgaga
     5881 actcgtcggg cggccgcgcc ggctcgtcgt agggggcgac gtggaggctc tcgacgccgc
     5941 cgacggccgc ggccagcgcc tcggtcgtca cgcgcaacat attgacataa ggatcggtta
     6001 tcgtcttgtt gcggcgggcc gtgcgggcat ggagccgcat gagcggcggg cgcatctttt
     6061 gcgcctcatc gtcgccgccg aaagcggcca cggcctgtga ccagagcagc cgcgcggcgc
     6121 gcagcttggc gatctccatg aagaagttgc cgccgacggc gaaatcgaag cgcatgtggc
     6181 cggcggcggc gtccacgtcc acgccgcgcc gctgcatggc ccgcagatag gccacaccgg
     6241 tggccagggc gaaggccaac tcttccacgg cgttggcccc ggcgttgtga taggggtagg
     6301 tgtggacggc gaccgtttgc aggcgcggcg cgtggcgctc ggcccagagg gtaacctggg
     6361 ccatctcgtc gtaggcgcgc cccagcgaca gggggatcgt cccctcgtgg gccagcacgc
     6421 ccagcggatc gtcttcgagg cagccgtgga ggtcggaagc cggtcgcccc gcccggcggg
     6481 cataggccac gagcagggcc agcaagggca gggcggccgt cccggcgcgg atggtgatcg
     6541 gcacggtcgc caggtcaacg ccgttgaggg cggcggccac gtcgtcgacg gaggccagcg
     6601 acaccccgcc acggccgacc tcgcccggct gggcggcgtc ggggtccttg ccggcgcggg
     6661 tcgggccgtc gagcagcagg ttggccaccg tctggccgtg ctccaaatcg aagcgcaggg
     6721 cgcgattaaa ggccgccggc gtgggacagg ccagctcctg ggcgatggcc catgggcgag
     6781 cgagataacc ggccgcgtcg ctgccgcgca tatagggcga tgcgccgggg atagttccgg
     6841 cggcggcgat atcgcccgtg tcttcgcgcc cgtaaagcgg ctggagcggg atgccctccc
     6901 aggtgggggt gatcagcttg tcgaacggtc gccccttcag cgagtcgacg gccgcctgct
     6961 gccattcgtc atacaatggc tcgggaaatt catcaaagga aagcatggga tcattccggc
     7021 gtgagtcgtc catagttttc aatattatac cagcgcggca tgcctgtcag gtgacgtgtg
     7081 tcaccttcag gcgcggtgaa tgtcatattc acgtttcggg gtttttcagt agccggctac
     7141 aacgtaacgc cggattcttg tccggcggcc ggggggattc accagtcagg aaacctgtgc
     7201 tacctgtatg ttccatgccc tataaggccg ctttgtacaa ttggaacgga ctactgaaaa
     7261 gccctgttca tgtttgcgcc tcctttacaa ctgagtgaaa actattttat aatgccccca
     7321 ggcgcatatc ggagagttcc catgccacct ttcgacgaag atgcgggcca atcgggaacc
     7381 ggtttggcct cgatagaagc ccagggagcc acccctggcg atgaaaggat cgaggcgatg
     7441 cccggccatg cccgcgagaa cctggccacg cccttcgcca taaccccaac cccgccggcg
     7501 ccacaagtcc acgacgttgt ccgccggcgg cctggcccat tcccggagaa ttatcatgtc
     7561 aactgactca atccatgccg tcaccggcgc gttcggcttt tccggcaaat atatcaccca
     7621 gcgcttgctg agcgaggggt gccgggtgat caccctcacc aactccgccg accgggccaa
     7681 cccgtttggc ggccgggtgg cagcctatcc cttccacttc gaacaacccg aactgctggc
     7741 ggaggcgctg gcgggggtcg agacgctcta caatacctac tgggtgcgtt tcaaccaccc
     7801 cctcttccgc cacgccgacg ccgtgcgcaa cacgctgatc ctcttcgagg ccgctcggca
     7861 gg
//
LOCUS       DAOMQG010000131         7862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3970_length_7862_cov_5.666837, whole genome shotgun sequence.
ACCESSION   DAOMQG010000131 DAOMQG010000000
VERSION     DAOMQG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_7862_cov_5.666837"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1453)
                     /locus_tag="PLC06_08685"
     CDS             complement(77..1453)
                     /locus_tag="PLC06_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90560.1"
                     /translation="MSLLDRMRRRLSVSADMREGAGGWSAIAGHPSAERPEAARQAQY
                     RDALEAWRSNPYAKRIIDLITDYTVGDGLTPAGEGEIGRFVDAFWNHPRNRLDLRLPE
                     LMDELSRSGDLFLALFRNAADGMSYVRAVPKGEVLEIVTAPNDWETEIAYVVRGGAPD
                     TKYEIRDTSEERDSELVSRISNLVSGGKVFLSPNHPDAAAADVVMVHYAINRPVGALV
                     GESELAGILPALRWYNRMLEDRVRLNWAARAFLWFVTVPTGRVHAKAEQYATPPEPGS
                     IVVHDEGERWEMVAPNLRGLDARHDLRALRQAISAGSGQPPHWHGDGGDVNRAVAAAM
                     QDPALRRLRRRQKQLQHLVIDLCHTAYTRAYETGTARRRPDRSAVRVALPDISREDNE
                     SLALAAERLTGAFAGLMGALPGWSPTLAGQMLPLIFKFAGEPMSAGLTQMILDELGDT
                     KYEIRNTS"
     gene            complement(1534..1722)
                     /locus_tag="PLC06_08690"
     CDS             complement(1534..1722)
                     /locus_tag="PLC06_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90561.1"
                     /translation="MNPIELLLRSRKFWLAVVAVIQTVVFSLIPDFPDEVWAAINVIL
                     LWLIGTIAAEDAASKIRN"
     gene            complement(2046..2561)
                     /locus_tag="PLC06_08695"
     CDS             complement(2046..2561)
                     /locus_tag="PLC06_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90562.1"
                     /translation="MNKQRPLLLIILILLSALLAGCGGLAAVPDSRPIPEEGVTPAPV
                     PPTEEPAKGDEEGLEALGGGLEWRRVGGIAGFCDLVIVDALGATVSTCRTEPPEIRAN
                     LTLTAAQAKQMQSWLDALAPFEHEQSDGAVADGMTITLTFAGQGTAQPTADDIAAMEA
                     LVMELLRAGAN"
     gene            complement(2607..2891)
                     /locus_tag="PLC06_08700"
     CDS             complement(2607..2891)
                     /locus_tag="PLC06_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002799383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRO90563.1"
                     /translation="MRELVLTPRFRRAYRKFVKRDRTLQKRIDDALESMQADVFTPGL
                     GSHKLSGELSGLWACSCGYDCRIIFAMEMESNTGREVIVLLDIGTHAEVY"
     gene            complement(2888..3124)
                     /locus_tag="PLC06_08705"
     CDS             complement(2888..3124)
                     /locus_tag="PLC06_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008205633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90564.1"
                     /translation="METNMVTLDQAIDTVMQLPAEQRVMLLEILQSRQIEALRHRIAA
                     DAQESLAAFRRGELTAQPAADVIAELCEMLEDEE"
     gene            complement(3167..6370)
                     /locus_tag="PLC06_08710"
     CDS             complement(3167..6370)
                     /locus_tag="PLC06_08710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRO90565.1"
                     /translation="MTALPPDFTHLHAHSHYSLLGGIGAPEELAARARADGLRALALT
                     DSNALFGAVAFARACRAEGIRPITGMTVSVAPPPGEMAPDTGNPLGCLVLLAAGPPGY
                     RSLCRLSSLYQSDPDRGRRARGGFSWDELRDNTAGLIALSGGRTGWLERYLRAGQAAA
                     ATRYVSRLGGLYGEQLCLAVGPDLVTGHAPLAGEIVALAQRFGLRAAAVQPIFCLSPD
                     DRPLLRLMAAIDANCRVEAVDPGRLPDGGDPDSPVEWPDPAAFAAAWANRPELLNAAG
                     EVAAQCGEALPDGRAIWPSVGVADPVRALREAAAVGMAARYGDPAPPAVAGRLAHETA
                     IIAQSGFAPLFLVVADVVRFARAAGIPAGTRGSVANSLAAYCLGITTVDPVAHDLLFE
                     RFLNPARRNPPDIDLDFCSRRRDEILDYVRRTWGADRVALVSTINTFQPRSAVRETAK
                     ARGLPEAAINQLVAVLPHSWHPDPRRRSADGLAEAMAQLDDSQLQDVLREAWRLVGRP
                     HHPGLHPGGVVITPGPLTDVAPLLLSPKGFLAVQYEHGDAEAIGLAKLDFLGIRALTV
                     LADALDLTGGRSEASVGQIASGAHLSGLESIPTDDPITAAMLSAGETIGVFQCESDGA
                     RRTLRQLRARNVADLAIAGAFFKPGPALGGMARAFVRRYRGEERVTYLHPALEPILGR
                     TKGVLIFQEQVLRLAVEVAGLDWGRADRLRRGISKFNAAEIDALAAEFIAGCGRPAPD
                     GPAMTAEQATRLWEQIVPFAGYGFNQGHATAYAEVAWRMAWLKAHWPAEFLCARLADA
                     GGFHHPEVYIAEARRLGIAVRPPHVNRSGEAFTLTHEPPETGFFPENTVSNQPILWMG
                     LGAVRDLRRASVAAIVAERARAPFSGPADLMARAGLSPKETANLVRCGALDGLGANRA
                     AMLDAAGAAGRAGSANQMRFDFLDGPTIAAESAADRLAWELEILDRPASVHPLELVAR
                     REDDTAVRALPQTRGRRVTALVVRIPGWPGGAGFFMSDGDDFVIARPAKALAEEKTRW
                     PAWQPLRLSGRWRVDEWGGGWFEVEAYELQATPDSPL"
     gene            6553..7824
                     /locus_tag="PLC06_08715"
     CDS             6553..7824
                     /locus_tag="PLC06_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRO90566.1"
                     /translation="MDALIKLQEIAVHMGLEPAEEVAAGGRQPTVEKGRKGAGEKGGK
                     GESIIPSPLPPLSLSPPHPFTPAPPPPCSPSSLGVTNVHLPNGKMMPLLKTMMTTACE
                     RNCFYCPFRAGRSATRRVTFSPDEMARAFHDMGRAGVVDGLFLSSGIIGGGVRAQDKL
                     LDTVEIVRRQGYRGYIHLKIMPGAERDQVLRAMQLADRLSINLEGPTPERLARLAPKK
                     QFIEELLPPLRWMEEIRRERPAHENVKGRWPSSATQFVVGAADESDLELLSATHHLTH
                     AAGLRRAYFSAFHPLRDTPLENHPAENPWREHRLYQASFLFRDYGFDLEEMPFDDQGR
                     LPLDTDPKRAWAAVHLGDAPVELNRADREELLRVPGIGPKGAAAILAARRRATLRSVD
                     ELRQIGVRTKGIEPFVLLDGRRPEYQMRLFT"
BASE COUNT         1337 a         2658 c         2614 g         1253 t
ORIGIN      
        1 tacgagatac gagatacgag atacgagata cgagatacga gatacgagat acgagttaag
       61 aggggcgctc tgcttcctaa ctcgtatttc gtatttcgta tttcgtatct cccaattcat
      121 ccagtatcat ctgcgtcaac cccgcgctca tcggttcgcc ggcaaatttg aagataaggg
      181 gcagcatttg gccggcgagg gtgggcgacc agccgggcag cgcgcccatg aggccggcaa
      241 acgcgccggt caatcgctcg gcggccagcg ccaggctctc gttgtcctcg cggctgatgt
      301 cgggcagcgc cacgcgaacg gccgagcgat ccggccgccg ccgcgccgtg cccgtctcat
      361 atgcccgcgt gtaggcggtg tggcacaggt cgatgaccag atgctgcagc tgcttttgtc
      421 gccgccgcaa gcgccgcagc gccgggtctt gcatcgccgc ggccacggcc cggttgacgt
      481 cgccgccgtc gccgtgccaa tggggcggct ggccgctgcc ggcgctgatc gcctggcgca
      541 gcgcgcgcaa gtcgtggcgc gcatccagcc cgcgcaaatt cggcgcgacc atctcccagc
      601 gctctccctc gtcatggaca acgatgctgc ccggctccgg cggcgtggca tactgctcgg
      661 ccttggcatg gacgcgcccc gtcggcacag tgacaaacca caggaaggcc cgcgccgccc
      721 aattgagccg cacgcggtct tcgagcatcc ggttgtacca gcgcagcgcg ggcaaaatac
      781 cggccagctc cgattcgccc accagcgccc ccaccggccg attgatggcg taatgcacca
      841 tcaccacatc ggccgcggcc gcgtcggggt ggttggggga gaggaagacc tttccgccag
      901 atacgagatt agagatacga gatacgagtt cagagtcgcg ctcttcactc gtatctcgta
      961 tttcgtattt cgtatccggc gctccgccgc gcaccacata ggcaatctcc gtctcccaat
     1021 cgttcggagc cgtcacgatc tccagcacct cgcccttggg cacggcgcgg acatagctca
     1081 tgccgtcggc ggcattgcgg aacagggcca ggaacaggtc gccggagcgg ctcagctcgt
     1141 ccatcaactc cggcaggcgc aaatcgagcc ggttccgggg gtgattccag aacgcatcga
     1201 caaagcgacc gatctcgccc tcgccggcgg gcgtcagccc gtcgcccacg gtgtagtcgg
     1261 tgatcaggtc gatgatccgc ttggcatagg ggttggagcg ccaggcttcc agcgcgtcgc
     1321 gatattgcgc ctggcgcgcc gcctccggcc gctcggccga tggatgaccg gcaatcgccg
     1381 accacccccc tgccccctcg cgcatgtcgg ccgaaacgct caaccgccgc cgcattcgat
     1441 ccagtaatga catagtgctc ctgatatcag atacgagata cgaaatacga gatacgagtt
     1501 cagaggcgcg ctcttcactc gtatctcgta tctctaattt cgtatcttgc ttgccgcgtc
     1561 ttcggcggca atggtgccga tcagccacaa cagaatgacg ttgatcgccg cccacacctc
     1621 gtcggggaag tcggggatga gcgagaacac gacggtctga atgaccgcca cgaccgccag
     1681 ccagaacttg cggctccgca acaacaactc gatggggttc atgcgcgcgc tccttgatgg
     1741 aatcggccgc gttcggccgt cattgagcgc aacgatagct ggagagggag tggtggggct
     1801 attcgattat tgacgatgcg ggaaaaaatc aggaatcagg aatcaggaag gagacaaccg
     1861 ccgaccgacg acagatgatc gacagcacta tctaattcag gcgatttggc tccgtggata
     1921 cccgggggat gatcccggat cgacaatcgg ctcgctattc gtccattgac cacggacgac
     1981 agaccacgca actcccgtcg cgcgaccacg gcaagagcgc gcccctcatt cgtaattcct
     2041 gattcctaat ttgcccccgc ccgcagcagt tccatcacca gcgcctccat cgccgcgatg
     2101 tcgtcggccg tcggctgggc ggtgccttgc ccggcgaagg tgagcgtgat ggtcatcccg
     2161 tcggccaccg cgccgtcgct ctgctcgtgt tcgaaggggg ccagggcgtc gagccatgac
     2221 tgcatctgtt tggcctgcgc ggccgtcagc gtcaggttgg cgcgtatctc cggcggctct
     2281 gtgcggcaag tggacaccgt cgcccccagc gcgtcgacga tcaccagatc gcagaacccg
     2341 gcaatgccgc ccacgcgccg ccattcaagg ccgcccccca gcgcctccag cccttcctcg
     2401 tcccccttgg ccggctcctc ggtcggcggc acgggggcgg gcgtcacgcc ctcctcgggg
     2461 atcgggcggc tgtcgggcac ggccgccaac ccgccgcagc cggccagaag cgccgatagc
     2521 aggatgagta tgatgagcaa aagggggcgt tgtttgttca tggtgttctt tatgttacga
     2581 gttacgaacg gatctcattc accggattaa taaacttcgg catgcgttcc aatatcaagc
     2641 aacacaatga cttctcggcc ggtattcgat tccatttcca tagcaaagat gatgcggcaa
     2701 tcgtagccac aagaacacgc ccataggccg gataattcgc cgctcagctt gtgggagcca
     2761 aggcccggcg tgaagacatc cgcttgcatc gattccagcg cgtcatcgat gcgcttttgg
     2821 agtgtgcgat ctctctttac gaatttgcga taggcgcgtc tgaacctggg cgtgaggacg
     2881 agttccctca ttcctcatcc tcaagcatct cgcagagttc ggcgatgaca tcggccgcgg
     2941 gttgcgccgt caattcacca cgacgaaacg ccgccagcga ttcctgggca tcggctgcta
     3001 tcctgtgccg cagcgcttca atctgccgac tttggagaat ttccagcagc atcacgcgct
     3061 gctcggccgg caattgcatg accgtgtcga tggcctgatc cagtgtcacc atgttcgtct
     3121 ccatagtccc ctccgcttaa gtatcacaaa gcgtttgccc attatatcat agcggagaat
     3181 caggcgtagc ttgaagctca tacgcctcca cctcaaacca gcccccgccc cactcatcca
     3241 cccgccaacg gccgctcagc cgcagcggct gccatgcggg ccaacgggtc ttctcctcgg
     3301 ccaacgcctt cgccggacga gcgatgacga agtcgtcgcc gtcgctcatg aagaagcccg
     3361 ccccgcccgg ccagccgggg atgcgcacga cgagcgccgt cacgcggcgg ccgcgcgtct
     3421 gcggcagggc gcgaacggcc gtgtcgtcct cgcgccgcgc caccagttcc agcgggtgga
     3481 cgctcgccgg gcggtcgaga atctccagct cccaggccag ccggtcggcg gccgattcgg
     3541 cggcgatggt cgggccgtcg aggaagtcga agcgcatctg gttggcgctg cccgcgcggc
     3601 cggccgcccc cgccgcgtcg agcatcgccg cgcggttcgc gcccagcccg tcgagcgccc
     3661 cgcagcgcac caggttggcc gtctctttgg gcgacagccc ggcgcgggcc atcaggtcgg
     3721 ccgggccgct gaacggcgcg cgcgcccgct cggcgacgat ggccgccacc gacgctcgcc
     3781 gcaggtcgcg caccgcgccc agacccatcc ataatatggg ttgattggaa accgtgtttt
     3841 ccggaaaaaa cccggtttcg gggggttcat gcgttagcgt gaacgcctcg ccgctgcggt
     3901 tgacgtgggg cggccgcacg gcgatgccca gccgccgcgc ctcggcgatg tagacctccg
     3961 ggtgatggaa gccgccggcg tcggccagcc gggcacagag gaactccgcc ggccagtggg
     4021 ctttcagcca ggccatgcgc caggctacct cggcgtaggc cgtggcgtgg ccctgattaa
     4081 agccataacc ggcgaagggg acgatctgtt cccacaggcg ggtggcctgt tcggcggtca
     4141 tggccgggcc gtcgggcgcg gggcggccgc agccagcgat gaattcggcc gccagcgcgt
     4201 cgatctcggc cgcgttgaat ttgctgatgc cccgccgcag ccggtcggcg cgcccccagt
     4261 ccagcccggc gacctccacc gccagccgca gcacctgctc ctggaagatg agcaccccct
     4321 tggtgcggcc gaggataggt tccagcgccg gatgcaggta ggtgacccgc tcctcgcccc
     4381 gatagcggcg cacgaaggcg cgggccatgc cgcccagcgc cggaccgggc ttgaagaacg
     4441 ccccggctat ggccaggtcg gccacattgc gcgcccgcag ttgtcgcaat gtgcgccgcg
     4501 ccccgtcgga ttcgcactgg aacacgccga tcgtctcccc cgccgacagc atggccgccg
     4561 taatggggtc gtcggtgggg atgctctcca gacctgacag gtgggcgccc gacgctattt
     4621 gtccaaccga agcctcactg cgcccacctg tcaggtccag cgcatcagcc agcaccgtca
     4681 gggcgcggat gcccaggaaa tcgagcttgg ccagtccgat ggcttccgcg tcgccatgct
     4741 catactgcac cgccaggaac cccttgggcg acagcagcag cggggccacg tcggtcagcg
     4801 ggccgggggt gatgaccacg ccgccggggt gcaggccggg gtgatggggg cggccgacca
     4861 gtcgccaggc ctcgcgcaat acgtcctgca actgcgaatc gtcgagctgg gccatcgcct
     4921 ccgccaaccc gtcggccgaa cggcggcgcg ggtcggggtg ccagctatgg ggcagcacgg
     4981 ccaccagttg gttgatcgcc gcctccggca gcccgcgggc cttggccgtc tcgcgcacgg
     5041 ccgagcgcgg ctggaaggtg ttgatcgtgc tgaccagcgc cacccgatcc gcgccccagg
     5101 tgcggcggac gtaatcgagt atctcgtcgc ggcggcggct gcaaaagtcg aggtcgatgt
     5161 cgggcggatt gcgccgggcg ggattgagga agcgctcgaa cagcaggtcg tgggccaccg
     5221 gatcgaccgt ggtgatgccc aggcagtagg cggccagcga gttggccacg ctgccacgcg
     5281 tgccggccgg aatccccgcc gcgcgggcga agcgtaccac gtcggccacc accaggaaaa
     5341 gcggcgcaaa gccggattgg gcgatgatcg ccgtctcatg ggccaggcgg ccggccaccg
     5401 cgggcggggc gggatcgcca tagcgggcgg ccatgcccac tgccgccgcc tcgcgcagcg
     5461 cccgcaccgg gtcggcgaca cccaccgacg gccagatggc gcggccgtcg ggcagcgcct
     5521 ccccgcattg cgcggccacc tcgcccgcgg cattgagcag ctccggccgg ttggcccagg
     5581 cggcggcgaa ggcggccggg tcgggccatt cgacgggcga atcggggtcg ccgccgtcgg
     5641 gcagccggcc gggatcgacc gcttccacac ggcagttggc gtcgatggcg gccatgaggc
     5701 gcagcagcgg ccgatcgtcg ggcgacaggc agaagatggg ctggacggcg gccgcgcgca
     5761 ggccaaagcg ttgggccagg gcgacgattt cgccggcgag gggcgcatgg ccggtcacta
     5821 aatccggccc gacggcgagg cacaactgct cgccatacaa cccgcccagc cgggacacgt
     5881 agcgggtggc ggcggccgct tgcccggccc gcagatagcg ctccagccaa ccggtgcggc
     5941 cgccgctgag ggcgatcagc ccggcggtgt tgtcgcgcag ttcgtcccag gagaagccgc
     6001 cgcgggcgcg acggccgcgg tcggggtcgg actgatagag cgaggagagg cggcacagcg
     6061 agcgatagcc cggcggcccg gcagccagca gcaccaggca gccgagggga ttgcccgtgt
     6121 cgggggccat ctcccccggc ggcggggcga cggaaacggt cataccggtg atggggcgga
     6181 tgccctcggc gcggcaggcg cgggcgaagg cgaccgcgcc gaacagggcg ttgctgtcgg
     6241 tcagggccag cgcgcgcaag ccgtcggccc tggcgcgggc ggccaactcc tccggcgcgc
     6301 cgatgccgcc cagcaaggaa tagtgggagt gggcgtgaag atgggtgaag tccggcggca
     6361 gggcggtcat ggggtgtggg gggattatag agtgacgggg ggcgagtgac gagtgacgag
     6421 tggtcagtgg cccgcagcca gtggccagac ggatcggaag ccgttctgcc cgcgtgaatc
     6481 cgtgaaaatc cgtgtcatcc gtgttctatt cctctcattg acaaccggga acatctgttc
     6541 tattctacca ccatggatgc actcatcaaa ttgcaggaaa tcgctgtgca tatggggctg
     6601 gagccggccg aagaggtggc ggcgggcggc cgtcaaccga cggttgagaa agggagaaag
     6661 ggagcagggg agaaaggggg aaagggagag tcgatcatcc cttcacctct tccccctctc
     6721 tccctttctc cccctcaccc cttcacccct gctcccccgc ccccctgttc cccttcctca
     6781 ctgggcgtga ccaacgtcca cctgcccaac ggcaagatga tgcccctgct gaagacgatg
     6841 atgaccaccg cctgcgagcg caactgcttc tattgcccct ttcgcgccgg gcgcagcgcc
     6901 acccgccggg tgacgttctc gcccgacgag atggcccgcg ccttccacga catgggccgc
     6961 gccggggtgg tcgacgggct gttcctgagc agcggcatca tcggcggcgg cgtgcgcgcc
     7021 caggacaagc tgctcgacac ggtggagatc gtccgccgcc agggctatcg cggctacatc
     7081 catctgaaga tcatgcccgg cgccgagcgc gaccaggtgc tgcgggcgat gcagctggcc
     7141 gaccggctgt cgatcaacct ggaagggccg acgccggagc ggctggcgcg gctggccccg
     7201 aagaagcagt tcatcgagga gctgctgccg ccgctgcgct ggatggagga gattcgccgc
     7261 gagcgaccgg cgcacgagaa tgtgaaaggg cgctggccgt catcggcgac ccagtttgtg
     7321 gtcggcgcgg ccgatgagag cgacctcgaa ttgctgagcg ccacccacca tctgacccac
     7381 gccgccggcc tgcggcgagc ctacttctcc gccttccacc ccctgcgcga cacgccgttg
     7441 gagaatcacc cggccgagaa cccctggcgc gaacatcggc tctatcaggc ctcgttcctg
     7501 ttccgcgact acgggttcga cctggaagag atgccgttcg acgaccaggg ccgcctgccg
     7561 ctggacaccg accccaagcg ggcctgggca gccgtgcatc tgggcgatgc gccggtggag
     7621 ctgaaccgcg ccgatcgcga ggagctgctg cgcgtgccgg gcatcggccc gaagggcgcg
     7681 gcggcgattc tggcggcacg gcggcgggcg acgctgcgct cggtggacga actgcgccaa
     7741 atcggcgtgc gcaccaaggg catcgagccg tttgtgctgc tcgacgggcg gcggccggag
     7801 tatcagatgc ggctgtttac ttgaattacg aattacgaat tacgaattac gaattgggag
     7861 cg
//
LOCUS       DAOMQG010000132         7845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_3983_length_7845_cov_8.708344, whole genome shotgun sequence.
ACCESSION   DAOMQG010000132 DAOMQG010000000
VERSION     DAOMQG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3983_length_7845_cov_8.708344"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLC06_08720"
     CDS             <1..501
                     /locus_tag="PLC06_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRO90567.1"
                     /translation="EVELKEKKHRVEDALSATRAAVEEGIVPGGGVALLNALPALDDV
                     KTPAGDISTGVNILRVALEAPMRKIAENAGLNGDVIIQNVRRLQAEKKDPKLGYNVMT
                     GEYPNMVKAGIIDPAKVTRGALENAASIAAMILTTEALVTDIPEDKKAPAMPGGGGMG
                     MGDMDF"
     gene            789..1856
                     /locus_tag="PLC06_08725"
     CDS             789..1856
                     /locus_tag="PLC06_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRO90568.1"
                     /translation="MNYDYAYADHLGSITGWSDAAGVYPSNSIALHEPFGAYRFRPPA
                     SVNPGISDRGYTGHRMNNSATNDLGLIYMNARYYLPEIGRFISADTIVPEPSNPQSYN
                     RYAYTYNNPVNYTDPSGHCIFGIDTAICVAAAIGAGVSLIVDYSFQAYDNYQAGMDAS
                     EALDPRNMNLSQLAGSTVAGGVGGVIAGPIGGLASKSSSLLARIGLTAAGGAIGGAVG
                     GQAGNLTQATVTEISDYYRGHGFNSDELFRQATEVGFLDPSTMMLDASTGAAFAVAGE
                     SLRSIVSQVSGSGGSKSLPIIGFEPVLGKGIQGKIYYQNRELNLTPSQLQALLQALSQ
                     NSRDAAIDLFVEWFGASLLED"
     gene            2336..2941
                     /locus_tag="PLC06_08730"
     CDS             2336..2941
                     /locus_tag="PLC06_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HRO90569.1"
                     /translation="MFAISHTRTMPSISNMIATYTCNGNMTTRTVNGVTYAQAFDVEN
                     RLTSVTVNGQVTSFHYDADGNRTLTVQPNGTKVYTPFPDYEEAVPASGGATKRVAFSL
                     AGQLIAVRVITDTTNNYYYAYADHLGSITGWSGAAGVYLSNSIALHEPFGAYRFRPPA
                     SVNPGISDRGFTGHRKKSPISTSIPPPLQPQEKSDDGRRQG"
     gene            complement(2882..3949)
                     /locus_tag="PLC06_08735"
     CDS             complement(2882..3949)
                     /locus_tag="PLC06_08735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRO90570.1"
                     /translation="MAPAPMIWLIIPLASAVTALVATPLARRLSFALGLVAVPGGRRK
                     HKGQIPKLGGLAVFVAWLVGVALAWWLAPPAAPDDALRLRGVVLGSFVMVAGGLLDDR
                     YDLPPGIQFAIQLLGALVAIFHIIFIELFTNPLPTATLWESLPLFRVEGELVWLWRPL
                     ALLFTVFWVVGMINAVNFFDGLDGLAVGVCLIAAAFFAFHSYRLQQTTVPLFALALAG
                     ALLGFLPFNFSPARIFLGSAGAWFLGYQMATLSILSPAKLSTALLVMAVPIIDVAWQI
                     VSRIRRGQHPMRGDRGHLHFRLSDRGLPTARIVLGYYAVAILFGLVAVLVASPLLKVA
                     TLLALAAAVIALLLRLERGRN"
     gene            complement(3953..4573)
                     /gene="upp"
                     /locus_tag="PLC06_08740"
     CDS             complement(3953..4573)
                     /gene="upp"
                     /locus_tag="PLC06_08740"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRO90571.1"
                     /translation="MVFESQHPLVKHKLTLMRDKETKPKKFRQLIREISMLLCYEATA
                     DLNTTPLVVESPMGRAEGVEMLDKVGLIPVLRAGLGMVEGIWEMMPGAEVWHIGLYRD
                     ERTLKPVSYYNKLPTNPTVQVCFVLDPMLATGGSAVATVDLLKKWGARRIKFVGILAA
                     PEGIARLSEAHPDVPIHIAHIDSHLNEIGYIVPGLGDAGDRQFGTA"
     gene            4846..5643
                     /locus_tag="PLC06_08745"
     CDS             4846..5643
                     /locus_tag="PLC06_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRO90572.1"
                     /translation="MNYSDYQHILFERREPHILWLTLNRPEVLNAADARLHTELVEVW
                     NTIDRDPDVHVAVVTGAGRAFSAGGDLALVENAWRNPEEIGRILDEARDLVYNILRCN
                     KPIVSAINGPAVGAGLVVALLADISIAAESARLADGHVRMGVAAGDHAAIVWPLLVGM
                     AKAKYYLMTAEFISGREAERIGLVSLCVPDGELLDRVMAVATQLATGPRHAIRYTKRA
                     LNQWLLQAGPIFDHSLALEMLGFFHEDMMAGVEALREKRAAKYPSSE"
     gene            5725..5988
                     /locus_tag="PLC06_08750"
     CDS             5725..5988
                     /locus_tag="PLC06_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRO90573.1"
                     /translation="MSSVGIRELKTNTSEIVRRVREQGEVVEITHRGEVVARLIPARP
                     IKSSPEELEALWADMDRLAEEIGAHWTGSPSAVDAVREDRREL"
     gene            5985..6404
                     /locus_tag="PLC06_08755"
     CDS             5985..6404
                     /locus_tag="PLC06_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO90574.1"
                     /translation="MTVTDASVWVSRFLPDDEFHEISRAWLKSVVGAGLPLFAPSHVL
                     AEVAGAMTRRTGRMGYGYAVAQRIQQVPTLQLVTITVETGDFAAEVASSYRLRGTDAL
                     YAAVAHQLNVSLVSWDKEQMMRVAGFITAYRPDEQIG"
     gene            6432..7496
                     /gene="paaA"
                     /locus_tag="PLC06_08760"
     CDS             6432..7496
                     /gene="paaA"
                     /locus_tag="PLC06_08760"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF010350.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRO90575.1"
                     /translation="MPANFTQKERRPMDERTDDGKLAEFEARIARGEKIEPGDWMPDA
                     YRKQLIRMISQHANSEVVGMLPEGAWITRAPNLRRKMVLLAKVQDEAGHGQYLYHAAE
                     TLGIGRDEMLDALHEGRAKYASVFNYPTLGWADMGAIGWLVDGAAIMNQTMLAHCSYG
                     PYSRAMVRICAEEGFHKKQGQEMIIRYAQGTPAQRQMAQEAIDRWWWPALMMFGPHDD
                     VSSNTPQLMRWGIKTKSNDQLRQEFIDELVPGLQALGLNVPDPEMHYDEATGHWAGGE
                     IDWDEFWRVIKGDGPMNAARLAARRAAHEDGRWVREAAAAYAQAQGRMGEREKGRKGE
                     REHSSTPSPLHPFSPASSLS"
     gene            7493..>7845
                     /locus_tag="PLC06_08765"
     CDS             7493..>7845
                     /locus_tag="PLC06_08765"
                     /inference="COORDINATES: protein motif:HMM:NF018002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein"
                     /protein_id="HRO90576.1"
                     /translation="MTDQWLKYEVFKQDRPGGPFHNVGSVHAPDAEMALMNARDVFAR
                     RPACVALWVVADSAIERRTAEELAGAQAGEGERGTENSVQYAVFSKQSQRQTMTYVDY
                     VGEVAAHSPNEAMRLA"
BASE COUNT         1652 a         2234 c         2473 g         1486 t
ORIGIN      
        1 gaggtcgagt tgaaagagaa gaagcaccgc gttgaggatg ccttgagcgc gacccgcgcg
       61 gccgtggaag agggtatcgt gcccggcggc ggcgtggctc tgctcaacgc cctgccggcg
      121 ctcgacgacg tgaagacccc cgcgggcgac atctccaccg gcgtcaacat cctgcgcgtg
      181 gcccttgagg ctccgatgcg caagatcgcc gagaacgccg gcctgaacgg cgacgtcatc
      241 atccagaacg ttcgtcgcct gcaagccgag aagaaagacc cgaagctggg ctacaacgtc
      301 atgaccggcg aatacccgaa catggtcaag gccggcatca tcgacccggc caaggtgacc
      361 cgcggcgcgc tggagaacgc ggccagcatc gcggccatga tcctgaccac cgaggcgctg
      421 gtgaccgaca tccctgaaga caagaaggct ccggccatgc ccggcggtgg cggcatgggc
      481 atgggcgata tggatttcta gttcgtcact aaaaaccggg tttctcggaa gaaactcggt
      541 ttttaatctc ttaatcccga cccaaaaagc cccctcgcgc gagggggctt ttgcttgcga
      601 ggtggatgat attgctcgcc aaatgatgtg acgtttgctg cttgacaagc cgtcactgag
      661 tgttattctg tcggcatcga gacgttccga gcgagggttc ccggaacaag cggctcgtca
      721 atcagtctgc gttcaagcct gtggggggat ggatgctaat agggcaatgg tcgggcaatc
      781 cgttgggaat gaattacgac tacgcctacg ccgaccacct ggggagcata acaggctgga
      841 gcgacgcggc cggagtctat ccgtccaatt ccatcgccct ccacgagccg tttggggcat
      901 accggtttcg gccgccggcc tcggtcaatc cgggcatcag cgacagaggc tataccggcc
      961 accgaatgaa caactcggcg acgaatgatc tgggcctcat ctacatgaat gcccggtact
     1021 acctgccgga gattgggcgg tttatcagcg cggatacgat tgtgccggag ccctctaatc
     1081 cgcagagcta taaccgatac gcctacacat acaacaaccc tgtcaattat actgacccgt
     1141 ccggtcattg tatatttggg atagatacgg ctatctgtgt agctgccgct atcggagcgg
     1201 gggtaagcct gattgttgat tatagctttc aagcttatga taattatcag gctgggatgg
     1261 atgcttcaga agcactagac ccgcgaaaca tgaatctttc tcaattggct ggttccacag
     1321 ttgccggcgg agtaggtggc gtcattgcag gccccattgg gggccttgca tcaaaatcct
     1381 cgtcattgct tgcgcgaata ggattgactg ctgctggagg tgcaatagga ggagcagttg
     1441 gggggcaagc cggaaaccta acgcaggcaa cagttacgga aatatcagat tattatcgcg
     1501 gccacggttt caattctgat gagctattca ggcaagcgac ggaagttgga ttccttgatc
     1561 caagtacaat gatgctggat gcctccacag gtgcagcatt tgctgtagct ggagagtcgc
     1621 tcaggtcaat tgttagtcaa gtatccggta gcggcggctc aaaatctctt ccgattatag
     1681 gttttgaacc agtacttggg aaaggaattc aaggcaaaat ctattatcaa aatcgagagt
     1741 taaacctaac accaagccaa cttcaagcac ttctacaagc tctatcacaa aactcacgcg
     1801 atgcagcgat cgatctcttt gtcgaatggt ttggagcatc cttgcttgaa gactaacctg
     1861 tgtgctcgtc ttcagttgcc acttcatgca aagagagatg gggctatgaa agagcgtaat
     1921 ttgttatttt ggatacttag agcattagtt tacataatta tatgtagtgt tgtaatcgca
     1981 agcctctgtg cgttcaaacc ttcggctcgc atcgctgccc tggttggatc actagtagga
     2041 gtattcttac ttccaatgac gtatcgctta gaaggcggga caatgactgc tttgggcgac
     2101 gtgctttttg cgactactac tttgttgttc gttatctcag taacaatatt tacgcccaca
     2161 atctgcgtag agtttgcaat gtacggcaac acttcgtgta acagagtgtc tagctcatgt
     2221 ctaattctta tgatcccagg cttattaatt gcctttttta tgtggctgtg gtttagatta
     2281 aggtagcggt ttagaagtag ggcgtgagaa ggacagttta gtaggatatc aaatagtgtt
     2341 tgcaatcagc catacccgca ccatgccgag tatcagcaac atgatcgcga cctacacctg
     2401 caacggcaac atgaccacgc gaaccgtcaa tggcgtcact tatgcccaag ccttcgatgt
     2461 cgagaaccgg ttgacgagcg tgacggtcaa cgggcaggtg acaagcttcc actacgacgc
     2521 cgacggcaac cgcaccctga ccgtgcaacc caacgggacg aaggtctaca cccccttccc
     2581 cgactacgag gaggccgtac cagcaagcgg tggagccacc aaacgggtgg ctttctctct
     2641 ggccggacaa ctcatcgccg tgcgcgtcat caccgatacc acgaataatt attactacgc
     2701 ctacgccgac cacctgggga gcataacagg ctggagcggc gcggccgggg tctacctgtc
     2761 caattccatc gccctccacg agccgtttgg ggcataccgg ttccggccgc cggcatcggt
     2821 caatccgggc atcagcgacc gcggcttcac cggccaccgc aagaaatcgc caatatccac
     2881 ttcaattcct cccccgctcc agccgcagga gaagagcgat gacggccgcc gccaaggcta
     2941 acagggtggc gaccttgagc aggggggagg ccaccagcac ggcgacaagc ccaaagagga
     3001 tggccacggc gtagtagccg aggacgatgc gcgcggtggg caggccgcgg tcggagaggc
     3061 gaaagtgcag atggccgcgg tcgccgcgca tggggtgttg gccgcgccgg atgcgcgaga
     3121 cgatttgcca ggcgacgtcg atgatgggca cggccatgac cagcagcgcc gtcgatagtt
     3181 tggcggggga gaggatggac agggtcgcca tctgatagcc caggaaccag gccccggccg
     3241 agccgaggaa gatgcgcgcc ggggagaagt tgaacggcag aaagcccagc aacgccccgg
     3301 ccagcgccag cgcgaacagg ggcacggtcg tctgctgcag ccggtagcta tggaaggcaa
     3361 agaaggcggc ggcgatgagg cacacgccga cagccaggcc gtccagccca tcgaagaaat
     3421 tgacggcgtt gatcatgccc accacccaga acacggtgaa cagcagcgcc agcggccgcc
     3481 aaagccacac cagttcgccc tcgacgcgaa agaggggcag cgattcccac aaggtggcgg
     3541 tcggcaacgg gttagtgaac agttcgatga agatgatgtg gaaaatggcg acgagcgcgc
     3601 cgaggagctg gatggcgaac tggatgccgg gcggcaggtc gtagcggtcg tcgagcaggc
     3661 cgccggcgac catgacgaag ctgcccagca ccacgccgcg cagccggagc gcgtcgtcgg
     3721 gggcggccgg cggcgcgagc caccaggcca gggcgacccc caccagccag gcgacgaaga
     3781 cagccaggcc gcccagcttg gggatttgtc ccttgtgctt gcggcggccg ccgggcacgg
     3841 ccacaagccc cagcgcgaac gacaggcggc gggccagcgg tgtggcgacc agcgcggtga
     3901 cggccgaggc gagagggatg atcagccaga tcatgggggc cggggccatt cgctaggctg
     3961 tcccgaattg ccggtcgccg gcgtcgccga ggccggggac gatgtaaccg atctcgttga
     4021 ggtgggagtc gatgtgggcg atgtggatgg gcacatcggg gtgggcttcg ctgaggcggg
     4081 ctatgccttc aggcgcggcg aggatgccga cgaatttgat gcgccgcgcg ccccatttct
     4141 tgagcagatc gaccgtcgcg acggccgatc cgccggtggc cagcatgggg tcaaggacga
     4201 agcagacctg caccgtgggg ttggtgggca gcttgttata gtaggacacc ggcttgagcg
     4261 tgcgttcgtc gcggtagagg ccgatgtgcc acacctcggc cccgggcatc atctcccaga
     4321 tgccttcgac catgcccagc ccggcccgca acacggggat gaggccgacc ttgtcgagca
     4381 tctccacgcc ttcggcccgg cccatggggg actcgacgac cagcggggtg gtgttgagat
     4441 cggccgtggc ttcatagcac agcagcatcg atatctcgcg gataagctgc ctgaatttct
     4501 tcggcttggt ttctttgtcg cgcatcaagg tgagcttgtg cttgaccaac gggtgttgtg
     4561 actcgaatac catcctgtct ctcctgtttc gggtgagggt attttagctg atatggggag
     4621 gaattaggaa ttaggaattg aaaacgactg ctgacgggcg accgacgacc gccgcaagag
     4681 cgtgctcttt atttgcactt cgtccttcgg ccttcgtact tctcttcact cgtcactttc
     4741 ttgcggcggt cggcggtctt tactcgtcac tcgtcattcg tcactcatat tggcggtatc
     4801 gccattattt tgctaatgtc ctgtatcagg agcgacgggc aacccatgaa ctatagcgac
     4861 taccaacaca tcctcttcga gcggcgcgag ccgcatatcc tgtggctgac cctcaaccgg
     4921 ccggaggtgc tcaatgccgc cgacgcccgg ctccataccg agctggtcga ggtttggaac
     4981 accatcgacc gtgaccccga cgtgcacgtg gccgtcgtca ccggcgcggg gcgagccttc
     5041 tccgccggcg gtgatctggc gctggtcgag aacgcctggc gcaacccgga agaaatcggc
     5101 cgcatcctcg acgaagcccg cgacctggtc tacaacatcc tgcgctgcaa caaaccgatc
     5161 gtctcggcca tcaacggccc ggcggtgggc gcggggctgg tggtggcgct gctggccgac
     5221 atcagcatcg ccgccgagag cgcccggctg gccgacggcc atgtgcgcat gggcgtggcc
     5281 gccggggacc atgcggccat cgtctggccg ctgctggtgg gcatggccaa ggccaaatat
     5341 tacctgatga cggccgaatt catcagcggc cgcgaggcag agcgcatcgg gttggtcagt
     5401 ctgtgcgtgc ccgacgggga gttgctcgac cgggtaatgg cggtggcaac ccagctggcc
     5461 accggcccgc gccacgccat ccgctacacc aaacgggcgc tcaaccagtg gctgctgcag
     5521 gccgggccga tcttcgacca ttcattggcg ctggagatgc tgggtttctt tcatgaggat
     5581 atgatggcgg gggtggaggc gttgagggag aagcgggcgg caaagtatcc gtcgagcgag
     5641 taagagcgct cgaaaaaccc ggtttctgtt gggttcatgt gttatacttt gtatgagact
     5701 acacatgtag gaacaggaat aatcatgtca tcagtaggca tccgtgaact gaaaaccaat
     5761 accagtgaga tcgtccgtcg cgtgcgcgaa cagggcgagg tcgtcgaaat cacccatcgc
     5821 ggtgaagtcg tggcccggct aattccggcg cggccgatta agagttctcc tgaggaactg
     5881 gaagcgcttt gggccgacat ggatcgcctc gccgaagaaa tcggcgcgca ttggaccggc
     5941 agccccagcg cggtggacgc cgttcgggag gacaggcgag aactatgact gtcaccgacg
     6001 ccagcgtgtg ggtcagtcgt ttcctgcctg atgatgagtt ccatgagatc agtcgcgcat
     6061 ggctgaaaag cgttgtcggt gcgggcttgc ctctctttgc gccttcgcat gtattggccg
     6121 aagtagccgg agccatgaca cggcgaacag gccgcatggg atatggatat gctgtcgctc
     6181 agcgcatcca acaagtgccg acattgcaat tggtgacgat taccgttgaa acaggcgact
     6241 ttgcggccga ggttgcctcc agttatcgac tacgcggcac tgacgcgctt tacgcggccg
     6301 tcgctcatca gctgaacgtg tcgcttgtca gttgggacaa ggaacaaatg atgcgcgttg
     6361 ccgggtttat caccgcatac aggcccgatg aacaaattgg gtagtgaccc gttttggtct
     6421 atactatttt cctgcccgca aatttcaccc aaaaggagcg ccggcccatg gacgaacgaa
     6481 ctgacgacgg taaactggcc gaatttgagg cccgtattgc ccgcggcgag aagatcgagc
     6541 cgggcgactg gatgcccgac gcttatcgca agcagctcat ccgcatgatc tcgcagcatg
     6601 ccaacagcga ggtcgtcggc atgttgccgg aaggggcgtg gatcacccgc gcgcccaatt
     6661 tgcggcgcaa gatggtgttg ctggccaagg tgcaggatga ggccggccac ggccagtatc
     6721 tctaccacgc ggccgagacg ctgggcattg gccgcgacga aatgctcgac gcgctgcacg
     6781 aaggccgggc caagtatgcc agcgtcttca actacccgac gctcggctgg gccgatatgg
     6841 gcgccatcgg ctggctggtc gacggcgcgg ccatcatgaa ccagacgatg ctggcccatt
     6901 gctcctatgg cccctattcg cgggcgatgg tgcgcatctg cgccgaagag gggttccaca
     6961 aaaagcaggg gcaggagatg atcatccgct atgcccaggg cacgcccgcc cagcgccaga
     7021 tggcccagga ggcaatcgac cgctggtggt ggccggcgct gatgatgttt ggcccccacg
     7081 acgacgtatc aagcaacaca ccgcagctca tgcgctgggg aatcaagacc aagtccaacg
     7141 accagttgcg gcaggagttc atcgacgagc tggtgccggg gctacaggcg ctggggctaa
     7201 acgtgcccga cccggagatg cattacgacg aggccaccgg tcattgggcc ggcggcgaga
     7261 tcgattggga tgagttctgg cgggtcatca aaggcgacgg cccgatgaac gccgcccgcc
     7321 tggccgcccg ccgcgccgcc cacgaagacg gccgctgggt gcgcgaagcg gcggcggcgt
     7381 atgcccaagc gcaggggaga atgggagaaa gggagaaagg gagaaagggg gaaagggagc
     7441 attcttcgac cccttctccc ctgcacccct tctcccctgc atcttcacta tcatgaccga
     7501 tcagtggctc aagtacgaag tattcaagca ggatcgcccc ggcggcccgt tccataatgt
     7561 cggctcggtg catgcgcccg atgccgaaat ggcgctgatg aacgcgcgcg acgtgttcgc
     7621 ccggcggccg gcgtgtgttg ctctgtgggt ggtggccgat tcggccatcg agcggcggac
     7681 ggccgaagaa ttggccgggg cgcaggcggg cgaaggagaa aggggaacgg aaaacagtgt
     7741 tcagtatgcg gtgttcagca agcagtcgca gcggcagacg atgacctatg tcgattatgt
     7801 gggcgaagtg gcagcccact cgcccaacga ggcgatgcgc ctggc
//
LOCUS       DAOMQG010000133         7790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4015_length_7790_cov_8.603103, whole genome shotgun sequence.
ACCESSION   DAOMQG010000133 DAOMQG010000000
VERSION     DAOMQG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4015_length_7790_cov_8.603103"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..896
                     /locus_tag="PLC06_08770"
     CDS             60..896
                     /locus_tag="PLC06_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90577.1"
                     /translation="MKSEQIVIEPVRGWQVINWRELRDYRDLFRFLVDRDIKVLYKQT
                     VLGFGWAILRPVLSMVIFTIIFGNLAQVDSDGVPYAIFSYVALVPWLYFSTALSASAL
                     SLVNNTEMLTKIYFPRLTFPMTPVFSKLVDFAISFVIVFVLMAWFRIMPTARMIYLPL
                     LILLMMLTAAGMGLWFAAMSVQYRDVKHAMPFLTQILMYAAPVVWAGSLITERFGQTG
                     RILFGLYPMVGVIEGFRSALLDTGPMPWDMIAVGTISALVIFVTGLFYFKRMEATFAD
                     VA"
     gene            919..2199
                     /locus_tag="PLC06_08775"
     CDS             919..2199
                     /locus_tag="PLC06_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90578.1"
                     /translation="MSSNGQAAGKAIIVEGVGKQYHIGLKEDRHDTLLKSAGAWLASP
                     VRNLRQLRRLSARDDDEAEDVIWALRDVSFSVDRGEVVGIIGRNGAGKSTLLKILSRI
                     TPPAEGRVILNGRVSSLLEVGTGFHPELTGRENVYLNGTILGMRKTEVDRAFDEIVAF
                     AEVEKFIDTPVKRYSSGMRMRLAFAVSAHLQSEILLVDEVLAVGDAAFQEKCLTKMEG
                     VTQIGRTVLLVSHNMQVVQRLCRRALLLQKGRLVAEGAPATIVGRYLSDALAATTLEP
                     VELGRQVRMNRLTVTQDGVPAGEFLDSERPFEVHVHYDILEPVRNLLLGFNVVAGDGI
                     ALFRTYDMLTYGLGERAPGAYESVFQLPGKLLPAGHYFFEVVVGIHRLRWLSKGDIRL
                     RLNLGGTRETDVDFPGVMSPVGSWAVYHNGDREH"
     gene            2202..3005
                     /locus_tag="PLC06_08780"
     CDS             2202..3005
                     /locus_tag="PLC06_08780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90579.1"
                     /translation="MAHFYWLSHRRYVDSGRMRWHAEQADEAFWAGYWRERVTPAYFR
                     KAADADLSADEMGRVLLAEMPVEGRHLEAGCGAGYWVAALRAAGRDVEGIEYARDLVE
                     LVNEVCPELPVRYGDALAIDCPDNTYASYLSFGVVEHRQAGPEPFLAEAYRVLAPGGK
                     LIITVPYLGPVRRLRARFGRYEATPPAPPFFQYGFRSGEFAGLVAGAGFVVETTRPLF
                     FHRMLLEENAAYRWLYDRRYGMVWRRALSGLFDGRDGHMLMVIAHKPAG"
     gene            3032..4951
                     /gene="asnB"
                     /locus_tag="PLC06_08785"
     CDS             3032..4951
                     /gene="asnB"
                     /locus_tag="PLC06_08785"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRO90580.1"
                     /translation="MCGICGELQFHDGPSRDGPSHDRETIAHLSELMARRGPDDAGLW
                     SDESRIWMAFRRLAILDLSPAGHQPMLSADGRFALVFNGEIYNFAELRDQLERLGARF
                     RSSGDTEVVLQALAHWGTAAPNRFNGMFALAFYDRRERELLLARDHAGIKPLYYLLGE
                     RGLFFASQYDQIMAHPWRADLPISADGLALYLRLGYIPAPYAILERTYMLGPGSWLRV
                     SAEGRVSRGTYYTFPMRGTPNLTGQEACEAVDAAVGDAVRRQMVSDVPLGAFLSGGID
                     SPLVVAKMRAAAGRPVRAYTIGSAGSELDESTDAADYARQLGVDHVLEQFTPDDALGW
                     LDEIVAACGEPLADHSIFPTMLVSHLARRDMTVMLSGDGGDELFWGYPERFGSVLVAS
                     SDFRRARWQRNARWAARRYTGWGNGHENLRWPTIGHWYRQKHTRIAEPVLTDLFAAPP
                     AWPDECGLFEFDGWEADETAHWLRWNEFVGHLAMVLLKVDRASMRNSLEVRVPLLDRE
                     VIATAARVDWRSCLDLETNTGKLPLRWALSRHISRQTQAKRGFAIPMASWLRGPLRDV
                     YREKVVGRAELAGMALDRGAAERLLAAHMNGRDLSRSLWTLLSLALWEDRHLRPAARQ
                     RGYEHPVIVSSGASL"
     gene            4948..5778
                     /locus_tag="PLC06_08790"
     CDS             4948..5778
                     /locus_tag="PLC06_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRO90581.1"
                     /translation="MNEVDSPATETKWREVVRSVNHALRPTARVTLCGTPYTLRAEAR
                     PNGSDRDYAVLRRLARGKACILDVGANVGLTSLVMAKGDMAPNGHIYAFEASEAASRI
                     IRDNAALNDLAARITVVNALIAERSGLTIDFYGDAASGSASIIPGYLAHHHPLRKAAL
                     ALDDFVRDADVTPDLIKIDVEGAELRVIDGLTETLRAARPLAFIELHSWGDLAVSDTA
                     DSLIERLVPLGYRLIYLRTKEFITDTAVLVGRGRCHVLACPAESSFLSELQELDTTGL
                     "
     gene            5805..7031
                     /locus_tag="PLC06_08795"
     CDS             5805..7031
                     /locus_tag="PLC06_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO90582.1"
                     /translation="MSHSDPVSLQKTQPEEGVPLSFRSLMDANHLMALVVILGVLALG
                     LRAISLRRDIGETLVNPALLKAAMQAAAGTPVSATEAAEVLSNRGAVSAFQARAMSRI
                     AARAEQWTTAEAWLAQGVGDADAGYLAQFELCRLYWGQGRRDYAREACRDTDSSAYYW
                     LNRGYVADQNGERAEALAAFQMATTVDPKLTVAWQQLGHALFASGRYGEAILAYERVM
                     ALNPTPPADIFHSLSRAYLETGNLTMVRDVVNRGLMIYPMERVLYLVMADSYRQESDL
                     ETAESWYVRMLQRWPYDASIWARRGEVAAAAGRLRDAEGYYQEAVAIQPDDVGYWTGL
                     ATTAVAGDNKPLARDAYQKAMALRPDDAALWLEAGRYFAEAGWASDARTAFERVIELE
                     PDNTEALDRLAELDGQ"
     gene            7036..>7790
                     /locus_tag="PLC06_08800"
     CDS             7036..>7790
                     /locus_tag="PLC06_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90583.1"
                     /translation="MTAYPEPVFAARRDRPVTSTVGDWLLTFGIENPAGPTRSSTGVW
                     AAEAQGATWRLSRSAPGESWSGMPMTAVAAPGWSAWLFGELYATADPVEIIADVVEGR
                     SSPTILNGHFLLLAQDRQTGEWHIWTNRYATLHAYVAANGPRVAIGSFMPAVAGAVGR
                     DGLDWEGLTSFFGFGFFMGDRTHFQGVRILRPATHYRFDGDGRALGEARYWHWQHMPD
                     RARTYDETVDEFAALFGDVMSGLTADGRIAVPVS"
BASE COUNT         1295 a         2255 c         2580 g         1660 t
ORIGIN      
        1 acatgatgcg cacgcatcgc aggtagcggc caaggggatt ggtccgggaa atgacgcaaa
       61 tgaagtccga acaaatcgtg attgagccgg tgcgtggctg gcaggtgatc aactggcgcg
      121 agctgcgcga ttaccgcgac ctctttcgct tcctggtcga ccgcgacatc aaagtgctct
      181 acaagcagac agtgttgggg ttcggctggg ccatcctgcg accagtgctg agcatggtca
      241 tcttcacgat catcttcggc aatctggcgc aggtggacag cgacggcgtg ccctatgcca
      301 tcttcagcta tgtggccctt gtgccgtggc tctacttctc cacggcgctg tcggcctcgg
      361 cgctgagtct ggtcaacaat accgagatgc tgaccaagat ctattttccg cggctgacgt
      421 tcccgatgac gccggttttc tccaagctgg ttgattttgc catctctttc gttatcgtgt
      481 ttgtgctgat ggcctggttc cgcatcatgc ccaccgcgcg aatgatctat ttgccgctgc
      541 tcattttgct gatgatgttg acggccgccg ggatggggct gtggttcgcg gcgatgtcgg
      601 tgcaataccg cgatgtgaag cacgccatgc cgttcctgac ccagatattg atgtatgccg
      661 cgccggtcgt gtgggcgggg tcgctcatca ccgaacggtt tggccaaacg gggcgaatcc
      721 tcttcggcct ctacccgatg gtgggcgtca tcgagggctt tcgctcggcg ctgctcgaca
      781 ccggcccgat gccctgggat atgatcgccg tgggcacgat ctcggcgctg gtcattttcg
      841 tcaccgggct gttctatttt aagcggatgg aagccacttt cgcggacgta gcctaaacag
      901 aagataatat tgatgaacat gagcagtaac gggcaggcag ccggcaaggc tatcatcgtc
      961 gaaggcgtgg gcaaacaata ccacatcggc ctgaaggaag accggcatga taccttgctg
     1021 aagtcggctg gagcatggtt ggcgtcaccg gtcaggaact tgcggcagtt gcgccgcctc
     1081 agcgcccgtg acgacgacga agccgaagat gtcatctggg ccttgcgtga tgtgtcgttc
     1141 agcgtcgatc gcggcgaggt ggtgggcatc atcggtcgca acggggcggg caagagcacg
     1201 ctgctcaaga tcctgtcgcg catcacgccg cccgccgaag gtcgcgttat cctcaacggt
     1261 cgcgtctcca gcttgctcga agtcggcacc ggcttccacc ccgaattgac tggtcgcgaa
     1321 aacgtctatc tgaacggcac gatcctgggc atgcgcaaga ccgaggtcga tcgagccttt
     1381 gacgagatcg tcgccttcgc cgaggtggag aagttcatcg acacgccggt gaagcgctat
     1441 agcagtggta tgcgtatgcg actggccttc gccgtctcgg ctcatctgca atcggagata
     1501 ctgctggtcg atgaggtgtt ggctgtgggc gacgccgcat tccaggagaa atgcctgacc
     1561 aagatggaag gcgtgaccca aatcggccgc acggtgctgt tggtcagcca taacatgcag
     1621 gtggtccagc ggctctgtcg ccgcgccttg ttgttgcaga agggacggct cgtagccgag
     1681 ggagcgccgg cgaccattgt cggtcgctat ctgagcgacg ccctggccgc gacgacgctg
     1741 gaaccggtgg aactggggcg gcaggtgcgc atgaaccggc tgaccgtgac ccaggacggc
     1801 gttccggccg gggaatttct ggacagcgag cggccgtttg aggtccatgt ccactacgat
     1861 atcctggagc cggtgcgcaa tttgttgctt ggcttcaacg tcgtcgccgg ggacggcatt
     1921 gccctctttc gcacctatga catgctcacc tacggtttgg gtgagcgcgc gcccggcgct
     1981 tatgaatccg tattccaact tcccggcaag ctgcttcccg ccgggcatta tttctttgag
     2041 gtcgtcgtcg gcattcaccg gctgcggtgg cttagcaagg gagatattcg cctgcggctc
     2101 aatctgggcg gcacgcgcga gactgacgtt gatttccctg gcgtgatgtc gccggtgggt
     2161 tcctgggccg tctaccacaa tggcgaccgc gagcattgat catggcacat ttctactggc
     2221 tctcacaccg tcgttatgtt gatagtggcc gcatgcgctg gcatgccgag caagccgatg
     2281 aggcgttctg ggccggctat tggcgcgagc gggtgactcc agcctatttc cgcaaggcgg
     2341 ccgatgccga tctgtcggcc gacgagatgg ggcgggttct gctggccgaa atgcccgtcg
     2401 agggacggca tctggaggcg ggctgtggcg cgggatattg ggtagctgcg ttgcgagctg
     2461 ccggacgtga cgttgagggg atcgagtatg cccgtgacct ggttgaattg gtcaacgagg
     2521 tttgccccga gttgccggtg cgctacggcg acgcgctggc catcgactgc ccggataata
     2581 cctatgcgtc ttatttgtcc tttggcgtgg tggagcatcg gcaggccggc cctgagccgt
     2641 ttctggccga ggcgtatcgc gtgctggctc ccggcggcaa gctgattatc actgtgccct
     2701 atcttggccc ggtgcgccgg ttgcgcgccc ggttcggtcg ctatgaggcg actccacccg
     2761 cgccgccgtt ctttcaatac ggcttccgct ccggggaatt cgccggattg gtggccgggg
     2821 cgggcttcgt cgtcgagaca acgcgcccgc tgtttttcca ccggatgttg ctggaagaga
     2881 acgccgccta tcgctggctc tatgatcggc gctatggcat ggtgtggcgg cgtgctttat
     2941 ccggattatt cgatggccgc gacggccata tgctcatggt gatagcccac aagccagccg
     3001 gctagccggc aagaggcaag gcagacacga tatgtgcggc atttgtggtg aactgcaatt
     3061 tcatgacggc ccatcccgcg atggcccatc ccacgacagg gagacgattg cgcatctgtc
     3121 ggagttgatg gctcgtcgcg gccccgatga cgccggtctg tggagcgacg agagccggat
     3181 atggatggcc tttcgtcgct tggctatcct cgatctgtcg cccgccggtc atcagccaat
     3241 gttgtcggcc gatggccgtt tcgctctggt gttcaatggc gagatataca acttcgctga
     3301 actgcgcgat cagcttgagc gattgggcgc gcggttccgc tccagtggtg atactgaggt
     3361 ggtgttgcag gcgctggccc attggggcac ggcggcgccg aatcgattca acggcatgtt
     3421 tgcacttgcg ttctatgatc gtcgggagcg cgaactgctg ctggcccgcg accatgccgg
     3481 gatcaagccg ctctattatc tgctgggcga gcgcggcctc ttcttcgctt cgcagtacga
     3541 tcagattatg gctcatccgt ggcgcgccga tttgccgata tcggccgacg gcctggccct
     3601 ctatcttcgg ctgggctata ttcccgcgcc ctatgccatc ctggagcgca cctatatgtt
     3661 ggggcctggc tcgtggctcc gtgttagcgc cgaagggcga gttagccgcg gcacttatta
     3721 caccttcccg atgcgcggca cgccgaacct gaccggtcag gaagcttgcg aggcggtcga
     3781 cgccgccgta ggtgacgccg ttcgccgcca aatggtcagc gatgtgccgc tgggggcgtt
     3841 tctgtccggc ggtattgatt cgccgctggt ggtggccaag atgcgggccg cggccgggcg
     3901 gccggtgcgg gcttatacta tcggcagcgc cggcagcgag ctggacgaat cgaccgacgc
     3961 ggccgactac gcccgacaac tcggcgtcga ccacgtgctg gagcagttta cgcccgacga
     4021 cgcgttgggc tggctggatg aaatcgttgc cgcttgcggc gagccgctgg ccgaccattc
     4081 tatcttcccc acgatgctgg tatcccacct ggcacggcgc gatatgaccg tgatgctctc
     4141 cggcgacggt ggggatgagc ttttctgggg ctacccggag cgtttcggct cggtgctggt
     4201 ggcctcatcc gacttccgtc gcgctcgttg gcagcgcaat gcccgttggg cggcccggcg
     4261 ttataccggc tgggggaatg gtcacgaaaa tttgcgctgg ccgaccatcg gccactggta
     4321 tcgccagaag cacacccgca tcgctgagcc ggtgttgacc gatctgtttg ctgcgccgcc
     4381 ggcctggccc gacgaatgtg gcctatttga atttgacggc tgggaggcgg acgagacagc
     4441 ccattggctg cgctggaacg agttcgtcgg tcatttggcg atggtgctgc tcaaggtcga
     4501 tcgggccagc atgcgcaact cgcttgaggt tcgtgtgccg ttgctggatc gcgaggtaat
     4561 cgctacggcc gcgcgggtgg attggcgatc gtgtctggat ctggagacaa ataccggcaa
     4621 gctgccgctg cgctgggcgt tgtcgcgcca tatttcgcgc cagacgcaag ccaagcgcgg
     4681 cttcgccatc cccatggcct cctggttgcg cgggccgctg cgcgatgtgt atcgcgagaa
     4741 ggtggttggt cgggcagagc tggccgggat ggcgctcgac cgcggcgcgg ccgaacggct
     4801 attggccgct catatgaacg gccgtgatct cagtcgcagc ttgtggacat tattgagcct
     4861 ggcattatgg gaagatcggc accttcgccc ggcggcccgg cagcgaggct atgaacaccc
     4921 tgtaatcgta tcttccggag catccctatg aacgaagttg actccccggc gaccgaaaca
     4981 aaatggcgcg aggtggtgcg atcagttaat cacgcattgc ggccgaccgc tcgggtgacg
     5041 ctttgcggga caccttacac attgcgcgcc gaagcgcggc ccaatggcag cgatcgcgat
     5101 tatgccgtgt tgcgccggct ggcgcgcggc aaggcctgta ttcttgacgt cggcgcaaac
     5161 gtgggcctga cctcattggt gatggccaaa ggagacatgg cgccgaacgg ccacatatat
     5221 gcctttgagg cctcggaagc ggccagccgg attattcgcg acaacgccgc gctcaatgat
     5281 cttgccgctc gcatcacggt ggtgaatgcc ctcatcgccg agagatcggg attgaccatc
     5341 gacttttatg gcgacgcggc ttccggcagc gcttcgatca tccccggcta tctggcccat
     5401 catcaccctc tgcgcaaggc ggcgctggcg ctggatgatt tcgtgcgcga tgccgatgtg
     5461 acgcccgatc tgatcaagat cgacgtcgaa ggcgcggagt tgcgcgttat tgacgggctg
     5521 acagagacgc tgcgcgctgc gcggccgttg gcttttattg agctgcacag ttggggagac
     5581 ctcgccgtca gcgacacggc cgactcgctg atcgagcgat tggtcccgct gggatatcgc
     5641 ctgatctact tgcgaacaaa ggaattcatc accgacacgg cggtgctggt cgggcgcggc
     5701 cgttgccatg tgttggcttg tccggctgag tcgtcgttcc tgagcgaact gcaggagctg
     5761 gacaccaccg ggctttagac ccacggatag tcggacgaga gacgatgagt catagcgatc
     5821 ccgtatcact tcagaagacg cagccggaag agggcgtgcc tctttcattc cggtcgctga
     5881 tggacgcgaa ccatctgatg gcgctcgtcg tgatcctggg tgttctggcg cttggcttgc
     5941 gcgcgatttc attgcgacgg gatatcggcg agacgttggt caaccctgcc ttgctgaagg
     6001 cggccatgca ggcggccgcg ggcacgccgg tttccgcgac cgaagcggcc gaggtgcttt
     6061 ccaaccgggg ggcggtgtcg gccttccagg cacgggccat gagtcgtatc gcggcgcgcg
     6121 cggagcaatg gacgacggcc gaggcctggc tggcgcaggg cgtgggcgac gcagatgcgg
     6181 gttatctggc acagtttgag ttgtgccggc tctattgggg ccagggacga cgcgactatg
     6241 cccgcgaggc ttgtcgtgat acggattcgt cggcctatta ctggcttaac cgtggttatg
     6301 tcgccgacca gaatggggag cgcgccgagg cgctggctgc tttccagatg gccaccacgg
     6361 tcgatccgaa gttgaccgtc gcctggcaac agcttggcca tgccttgttt gcgagcggtc
     6421 gctatggcga agcgatcctg gcctatgagc gcgtcatggc gttgaacccg actcccccgg
     6481 ccgatatatt tcattccttg agccgggcct acctggaaac cggcaacctg acgatggtgc
     6541 gcgacgtggt caatcggggc ctgatgatct atcccatgga gcgcgtcctc tatctggtga
     6601 tggctgattc gtaccggcag gagtctgacc ttgagacggc cgagagctgg tacgtgcgca
     6661 tgctccagcg ctggccatat gacgcatcga tatgggccag acgcggcgaa gtggccgcgg
     6721 ccgccggtcg tttgcgcgac gccgaaggat attatcaaga ggctgtcgcc atccagcctg
     6781 acgatgtcgg ttattggacg ggccttgcca cgactgccgt ggccggggac aacaaaccgc
     6841 tggcccgcga cgcctatcaa aaggcgatgg ccttgcgacc tgatgatgcc gctctatggc
     6901 ttgaggccgg ccgttatttc gccgaagccg ggtgggcttc cgacgcgcgg acggcctttg
     6961 agcgcgtgat tgaactcgaa cccgataata ccgaggcgct tgaccggctg gctgagctgg
     7021 atggtcaata acccgatgac agcgtatccc gaacccgttt ttgcggcccg acgcgaccgg
     7081 ccggtgactt ccacggtcgg tgattggctg ctgacttttg ggattgaaaa tccagccggc
     7141 cccacgcgtt cgtctacggg tgtatgggcg gcggaggcgc aaggcgctac ctggcgatta
     7201 tcgcgctccg cgccgggcga gagctggtcg ggcatgccca tgacggccgt tgccgcaccg
     7261 gggtggtcgg cctggctgtt tggcgagctt tacgccacgg ccgatccggt cgagattatt
     7321 gccgatgtgg tcgaaggccg ttcatctccg acaatcctga acggccattt cctgttgctg
     7381 gctcaggaca ggcagacggg tgaatggcac atctggacga atcgatatgc tacgctccac
     7441 gcctacgtgg ccgcaaatgg cccgcgtgtg gcgataggca gtttcatgcc cgccgttgcg
     7501 ggagctgttg gccgtgatgg gctggattgg gagggattaa ccagcttttt tgggttcggc
     7561 tttttcatgg gcgacaggac gcacttccag ggggtgcgta tcctgcgccc ggcaacccat
     7621 tatcgttttg acggcgatgg ccgcgcgctt ggcgaagcgc gttattggca ttggcaacat
     7681 atgccggatc gggcgcgaac ctacgatgag actgtcgatg agttcgccgc gctctttggc
     7741 gatgtgatga gtggcctgac ggccgatggc cgcatcgcgg tgcctgtctc
//
LOCUS       DAOMQG010000134         7782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4019_length_7782_cov_10.936715, whole genome shotgun sequence.
ACCESSION   DAOMQG010000134 DAOMQG010000000
VERSION     DAOMQG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_7782_cov_10.936715"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..156
                     /locus_tag="PLC06_08805"
     CDS             4..156
                     /locus_tag="PLC06_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRO90584.1"
                     /translation="MAGAILLPFVILISAIRLYLKRHTPAQVLAGLALGISTVLAMRA
                     FGCFVP"
     gene            166..945
                     /locus_tag="PLC06_08810"
     CDS             166..945
                     /locus_tag="PLC06_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90585.1"
                     /translation="MFEQERFVGRLQRYVQADAAVQVCFLHGSFGRRAEDAYSDVDCV
                     LVYEGQAEQEIAWGGRQEFVKGVNPYVAVRSFDAAHVRPWLHVVLYSNGTKADFAFER
                     MDALEPASIFREIRILKDSDRWAEQLQAASSRLAPPQPYISPAELTELDNRFWVMMWD
                     TLRLLKRGDSDKPFAIYLQTLYFTLPPLLAALPPEEPARRALMNATYSRDIAATTRGL
                     GDLLGAYLAARSAVIRRQNLMFPINTAFESEIRRLVERLTR"
     gene            complement(955..1533)
                     /locus_tag="PLC06_08815"
     CDS             complement(955..1533)
                     /locus_tag="PLC06_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRO90586.1"
                     /translation="MNTIKDQEDTLTISFKAELFKINSWTILRLPEDASARLPSRGMT
                     MVSGTMNGVPFKALLEPDGRYAPGQQSSHWFSPDKKLLDAAGAVAGDSVEVSMEPSKE
                     WIEPEVPEDLKEALSTSPKAEALWEDITPLARWDWIRWIRAVKTAETRQKHIEVALDK
                     LNKGMRRPCCFNRNLCSEPYVSKNWALLEPTQ"
     gene            1699..2142
                     /locus_tag="PLC06_08820"
     CDS             1699..2142
                     /locus_tag="PLC06_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRO90587.1"
                     /translation="MTTQNDSSVEDYIASLNDEQTAKDCLVLIEMIQRISGHEPKMWN
                     VGTIGFDTYHYKYDSGREGDAHVIGFYPRKGKITVYLMDGTARYSELLAKLGKHTTSG
                     YCVYIKRLRDIELPVLEQIVQQSYGYIKSKSQDGPIDRVLWQTEK"
     gene            complement(2172..>2801)
                     /gene="miaA"
                     /locus_tag="PLC06_08825"
     CDS             complement(2172..>2801)
                     /gene="miaA"
                     /locus_tag="PLC06_08825"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRO90588.1"
                     /translation="VGGTGQYVWAVVEGWGIPEVAPNPALRASLEALGQDELARRLAA
                     LDPTAAGRIDPRNVRRVVRALEVTLVTGRPISELQRKTPPPYDIIMLGLTRDRDTLYA
                     RIDARVERMMVDGLLDEVIRLGEAGYGPHLPSMSGLGYRQLLAHLAGEMTLEEAVERI
                     KFETHRFARQQATWFRGDDLRIRWFEADDTGAVVSAVEEWLRVTTDDRP"
     assembly_gap    2802..2901
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2963..3346)
                     /locus_tag="PLC06_08830"
     CDS             complement(2963..3346)
                     /locus_tag="PLC06_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRO90589.1"
                     /translation="MSESPSDPRRRLGQWGEKVAALHLEAQGLAILERNWRCRDGEID
                     LVARDGETVVFIEVKTRRGRDFGAPEEALTSHKAAKLMQLGQQYMADRELDDVDWRID
                     LVAVELDGNGKLLRCDHIPNAVLGW"
     gene            complement(3339..4634)
                     /locus_tag="PLC06_08835"
     CDS             complement(3339..4634)
                     /locus_tag="PLC06_08835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO90590.1"
                     /translation="MRIGMVSATYDATVVNGAVRMVTLYKEHLEALGHEVTIFTLGEP
                     EESDPAARIVRSPGIRLGGYGYYISMSYTREAQALLRQMDIVHCHHLLMSVELAHRYV
                     QCPIVYTNHTRYDLYTGTYTPLPQPAADAIMRQIWPEYTDLADVVIAPSDGVRRVMQE
                     FGVRSRIVVIENGVDLERFLHPYQPRTKADYGLPDSARLLVYIGRLATEKNIEALLTQ
                     FTAARVLIPDLYLALIGKGPQEAELKELAEELELGDAVQFRGVVEYDEVPNWLAAADG
                     FVTASTSEVHPLTVIEAMTTGLPIAAVRSPGISDSVESGVTGYLAADENGLDAAIVAL
                     ISDLSKAKVMGAAAREAGKRFDIHRTVAETVKLYEELLTARPDLQRAEKHGRWARRTE
                     KWGGLLNQLAEITHPVGDLDEVTRHWWPLDLTGPRKHNE"
     gene            complement(4663..5178)
                     /locus_tag="PLC06_08840"
     CDS             complement(4663..5178)
                     /locus_tag="PLC06_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRO90591.1"
                     /translation="MQPSIRTILSTTGIFDNFNDAQLTLVAALCTPVTYNQGHVLLSE
                     NDQSDEMYIIGRGGVEVLVNPAAVASGSDLQDMEPVVLTELRQGQIVGEVALVDQGVR
                     SATIRVSRDDTLLLRLRRDQLMRLGETYPVLGFKLMRNLATELATKIRNTDWLVRQYQ
                     LRLQSDESGSL"
     gene            complement(5198..5998)
                     /locus_tag="PLC06_08845"
     CDS             complement(5198..5998)
                     /locus_tag="PLC06_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90592.1"
                     /translation="MNDALMPLLWAALALICLLFLQRWIHAHLHGVSLLLVGRPEWAV
                     IVYAVVLFPGVLLHEASHWLAATLLGVRTGGVSLLPRRQADGSLQLGYVEYYKSPGVG
                     PIRESLIGAAPLIAGTIVILLIGHHVFSVTSLADAIRTADVAILADAMAQILAVPNVL
                     VWLYLIFAVSNAMMPSRSDRHAWPAFALIMGAVALVVGFLGRNTGLLNNLAQPVAVLF
                     GYLGTAFSIAIAVDLLCIGLIALAEWVLGRLRGASVVYGHSSNRETTT"
     gene            complement(6003..6758)
                     /gene="tpiA"
                     /locus_tag="PLC06_08850"
     CDS             complement(6003..6758)
                     /gene="tpiA"
                     /locus_tag="PLC06_08850"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRO90593.1"
                     /translation="MRTPFVAGNWKMNKTPDAAAAFVREIAPGLNAIDGIDIAVCPPF
                     LAIPAVAQVLAGTKIGVGAQNMYYEVSGAYTGEISPAMLQGLCRYVILGHSERRALFG
                     ETDEGVNKKIKAALAHNLTPIVCVGESLAQNEAGETQSFVSGQVRAAFAGLDSWQAAV
                     CVVAYEPIWAIGTGRSATAQQAGDIIRTSVRDVLADMFGNDTAQAIRIQYGGSVTETN
                     IADYMAQPDIDGALVGGASLKTSFIDLVRAAVI"
     gene            complement(6955..>7782)
                     /locus_tag="PLC06_08855"
     CDS             complement(6955..>7782)
                     /locus_tag="PLC06_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO90594.1"
                     /translation="VRIRQTQHENQIEGLQERIRADLGLVSLSYDAEQTGPHPLPIRE
                     VVEELPAVDELPPDIEENIHHYRGQLQRMGPINPDAPAELATTEERFEFLTQQVGDLT
                     ETDAQLRGVIAELDELTSKAFSKTVEEVNGIFDQTFQQLFGGGSGRLVMTDPDDPTSS
                     GVEIIARLPNRREQGLALLSGGERSLTAAALIFSLLKVAPPPFCVLDEVDAALDEANV
                     NRFRDVLRELGQHTQFILITHNRGTVQAAQTLYGVSMQPDSSSQVISIKPEEYLRRG"
BASE COUNT         1532 a         2268 c         2313 g         1569 t
ORIGIN      
        1 tgggtggccg gcgccattct tctgccgttc gtgatcctga tctcggccat ccgcctctat
       61 ctgaaacggc acacgccggc gcaggtgttg gctgggctgg cgctgggcat ctcgacggtg
      121 ctggcgatgc gcgctttcgg ttgctttgtg ccctgatcga cgatcatgtt tgagcaagaa
      181 cgattcgtag gtcgtctcca acgctatgtg caggcggacg cggccgtgca ggtgtgtttt
      241 ctgcacggtt ctttcggccg tcgtgccgag gacgcctatt cggacgtgga ttgtgttctt
      301 gtttatgagg gacaggcgga gcaggagatc gcctggggcg gccgtcagga gttcgtcaag
      361 ggcgtgaacc cctatgtagc cgtgcgctca ttcgacgccg cgcacgttcg cccctggctc
      421 catgtcgttc tctatagcaa cggaaccaaa gcggatttcg cctttgagcg catggatgcg
      481 ctggagccgg cttcaatttt ccgggagatt cgtatcctga aggatagcga ccgctgggcc
      541 gaacagttac aggcggcatc ctcccggctg gccccgccgc aaccttatat cagcccggcc
      601 gaactcactg aactggacaa tcgcttctgg gtgatgatgt gggacacatt gcgtctgttg
      661 aagcgcggcg attccgacaa gccgttcgcc atttacttgc agacgctcta cttcacgctg
      721 ccgccgttgc tggccgccct gccgcctgag gaaccggcgc gacgggcgtt gatgaacgcg
      781 acctacagcc gcgatatcgc tgccacgacg cgcgggctgg gcgacttgct gggggcgtat
      841 ctggccgcgc gctcggccgt cattcgccgc cagaacctca tgttccccat caataccgcg
      901 tttgaatcgg agatccgccg gctggttgag cggctcacgc ggtaaatcgt atagctattg
      961 tgtcggttcg aggagcgccc aattcttcga aacatacggt tcagagcaca ggttccgatt
     1021 aaagcaacac ggccttctca tccccttgtt gagtttatcg agcgcgacct ctatatgttt
     1081 ttggcgcgtt tccgccgttt tgacggcgcg tatccagcga atccagtccc aacgagcaag
     1141 tggcgtaata tcttcccata aagcctccgc ttttggagaa gtagacagcg cttcttttaa
     1201 gtcttcagga acttccggct ctatccactc tttggatggc tccatcgaaa cttccacact
     1261 atcaccagcc acagcgccgg cagcgtcaag cagctttttg tcaggactaa accagtgact
     1321 tgattgttgc cctggtgcat accttccatc cggttcgagc aaggctttaa agggaacgcc
     1381 gttcatggtc cctgaaacca tcgtcattcc tcttgatgga agcctggctg aggcatcttc
     1441 aggtagtctg agaatggtcc atgaattgat tttgaatagt tcagccttaa aactgattgt
     1501 cagcgtatcc tcttgatcct taattgtgtt cataagatga attctactaa gaatgccaaa
     1561 ccaaaactaa tcttatagaa tcgttgcatg gtcaagtcat tggtgagcga cgaatcggga
     1621 tgacctgttg gcgtcggttc cgggattgcc agacggccgg tgttaagttc cggcattttc
     1681 cataagatgg agcctgttat gacgactcaa aacgacagct cagttgaaga ctacattgct
     1741 tcacttaatg atgagcaaac cgcaaaagat tgccttgtgc ttattgaaat gatacagcga
     1801 ataagcgggc atgaaccgaa gatgtggaat gtcggcacta tcgggtttga tacctatcac
     1861 tacaaatacg acagcgggcg cgaaggcgac gctcacgtta ttggttttta tccaagaaaa
     1921 ggcaagatta ctgtttacct gatggacggc acggcacgct actctgaatt gctggccaag
     1981 ttaggcaagc atactacttc cgggtattgt gtttatatca agcggctgag ggacatagag
     2041 ttgcccgtcc ttgagcaaat cgtgcaacag tcttatgggt atataaagtc aaagtcccag
     2101 gacgggccta tagaccgggt gttatggcaa actgaaaaat aggcgtctct gcattaaacc
     2161 ggttctgcgg cctatggcct gtcgtctgtg gtcactctca accactcttc aaccgcagaa
     2221 acgactgctc ctgtatcatc agcctcgaac cagcggatcc gcaggtcatc gccgcgaaac
     2281 caggtcgcct gttgtcgggc aaagcggtgg gtctcgaact tgatcctctc gacggcctcc
     2341 tccagggtca tctctcccgc cagatgggcc agcagttggc gatagcccag gccgctcatg
     2401 gacggcagat gcgggccata gccggcttcg ccgagccgga tcacttcgtc gagcaaccca
     2461 tcgaccatca tcctttcaac acgagcgtca atgcgcgcat aaagcgtatc gcggtcgcga
     2521 gtcaggccaa gcatgatgat gtcataaggt ggcggcgttt tgcgctgcag ttcggaaata
     2581 ggccggccgg tgactagcgt cacttccaaa gcgcgcacca cgcggcgcac gttgcgcggg
     2641 tcgatgcgcc cggcggctgt cgggtcgagc gcggccagcc ggcgcgccaa ctcatcttgc
     2701 cccagtgcct ccagcgaggc ccgcagcgcc ggattcggcg cgacttcggg gatgccccaa
     2761 ccctcgacga cggcccacac gtattggcct gtgccgccaa cnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn ngccgtcttg cccacggccg tggggccaac gagaaccaaa
     2941 agtggaggag ctacgcgatc gcctaccacc ccagcaccgc gttggggatg tggtcgcagc
     3001 gcagcaactt gccgtttccg tccagctcca ccgcgaccag atcgatgcgc caatcaacgt
     3061 catccagttc gcggtcggcc atgtattgct gccctaattg catcagcttc gccgctttgt
     3121 gggatgtcag cgcctcttcc ggcgcgccaa aatcgcgacc tcgacgagtc ttaacctcga
     3181 taaaaacgac cgtctcgccg tcgcgggcaa cgagatcgat ttccccgtca cggcagcgcc
     3241 agttgcgctc gaggatggcc aggccctgcg cttccagatg aagcgcggcg accttttcgc
     3301 cccattggcc gaggcgacgg cgagggtctg acggtgactc actcattatg cttgcgcggg
     3361 ccagtcaaat ccagcggcca ccaatgccgc gtcacttcgt ccagatcgcc cacggggtgt
     3421 gtaatttcag ctaattggtt cagcaaccca ccccatttct ccgtgcgccg tgcccagcgg
     3481 ccgtgttttt cggcccgttg caggtcaggg cgcgccgtca acagttcttc gtagagcttg
     3541 accgtctcgg ctacggtgcg atggatgtca aatcgtttac ccgcttcacg ggcggccgcg
     3601 cccatcacct tcgccttact caggtcgctt atcagggcga cgatggccgc gtccagccca
     3661 ttctcatcgg cggccagata gccagttacg cccgattcaa cgctgtcgga aatgcccggc
     3721 gaacgcacgg cggcgatggg aaggccggtc gtcatggcct caatgaccgt cagcgggtga
     3781 acctcgctgg tagaggcggt gacaaatccg tcggccgcgg ccagccagtt gggcacttca
     3841 tcatactcca cgacaccgcg aaactgaacg gcgtcgccga gttcaagctc ctcggccaat
     3901 tccttcaact cagcttcctg tggccccttg ccaatcagcg ccagatacag gtcggggatc
     3961 agaacgcgcg cggcggtgaa ctgggtcagc aacgcctcga tgttcttttc cgtcgccagc
     4021 cggccaatgt aaacgagcag ccgggccgaa tccggcaagc cgtagtcggc tttggttcgc
     4081 ggttggtagg gatgaaggaa ccgctccagg tcaacgccgt tttcgatgac cacgatacgg
     4141 ctgcggacgc caaactcctg catcacccgg cgcaccccgt ctgacggggc gatgacgacg
     4201 tcggccaggt cggtatactc cggccatatc tggcgcatga tggcgtcggc cgccggttgc
     4261 ggcagaggcg tataagtgcc cgtgtacagg tcatagcggg tgtggttggt gtagacgata
     4321 gggcattgca cgtagcggtg ggccagctcg acgctcatca gcagatgatg gcaatggaca
     4381 atatccattt gtcgcaggag agcttgcgcc tcgcgcgtgt agctcatgct gatgtaatag
     4441 ccgtacccgc caagccggat gccgggggaa cgaacgatac gcgccgcagg atcagattcc
     4501 tcgggttcac cgagggtgaa gatagtaacc tcgtgcccca gcgcctcgag atgttcctta
     4561 tagagcgtca ccattcgcac ggccccattg acgactgtgg cgtcataggt ggccgaaacc
     4621 atgccgatac gcatactcct cctgatgagt gggccgacta tgttacaagc ttccggattc
     4681 gtctgattgt aatcgaagtt gatattgcct gacgagccaa tcggtattgc ggatttttgt
     4741 agccaactcg gtggccagat tgcgcattag cttgaagccc aggacagggt aagtctcgcc
     4801 cagccgcatg agttgatccc gccgcaggcg gaggagcagg gtgtcgtcgc gactgacgcg
     4861 aatggtggcc gagcgcacgc cctgatcgac cagagcaact tcgcctacga tctgtccctg
     4921 gcgcaactcg gtcagcacaa ccggctccat gtcctgtaga tcgctgccgg acgcaactgc
     4981 ggcaggattg accagcacct cgactccgcc acggccgatg atatacatct catccgattg
     5041 atcattctcc gacagcaaca cgtgtccctg attataggtg accggcgtgc agagcgcggc
     5101 caccagcgtc aattgcgcgt cgttgaaatt gtcgaaaatc ccggtggtcg acaggatggt
     5161 gcggatactt ggttgcatac agtgattctt tcctcgatca ggtcgtggtc tctctattgg
     5221 agctatggcc ataaacgacg ctggccccac gcaggcggcc gagcacccat tcagccagag
     5281 cgatgaggcc gatgcacagc agatcgacgg cgatggcgat agagaacgcg gtgcccagat
     5341 agccgaacag cacggcgaca ggttgagcca gattattcag caagcccgtg ttgcgcccca
     5401 gaaaaccgac gacgagggca accgcgccca taatgagcgc aaaggcgggc caggcgtggc
     5461 gatcggaacg actgggcatc atggcgttgc tgacggcaaa aatcagatag agccaaacca
     5521 gcacattggg cacggccaat atctgggcca tggcgtcagc gagaatggcg acgtcagcgg
     5581 tacggatggc gtcggcgagg ctggtgacgc tgaagacgtg atggccaatg agcaggatga
     5641 caatcgtgcc ggcgatcaag ggcgcagcgc cgatcaggct ctcgcgaatg gggccgacgc
     5701 cgggactttt gtaatactcg acatagccca actggagcga accgtcggcc tggcgacgtg
     5761 gcagaagcga cacgccgccg gtgcgcacac cgagcagggt agcggccagc caatgactgg
     5821 cctcatgcaa gagcacgccg ggaaagagca cgacggcata gacgatgacg gcccactccg
     5881 ggcggccgac cagcaacagg ctaacgccgt gcaggtgcgc gtgaatccag cgctgcagga
     5941 agagcaggca gatcagcgcc agggccgccc agagcaaggg catcaacgca tcattcattg
     6001 agttatatga cggcagcgcg gacgagatcg atgaacgacg tcttgagcga cgcgccaccc
     6061 accagcgcgc cgtcgatgtc gggctgcgcc atgtagtcgg cgatatttgt ctcggtcacg
     6121 ctgccgccat actggatgcg aatggcctgg gcggtgtcgt tgccgaacat atcggccaac
     6181 acatcgcgaa cgctggtgcg gatgatatca cccgcctgtt gggccgtggc cgagcgaccg
     6241 gtgccgatgg cccatatcgg ttcgtaggcg acgacgcaca cggccgcctg ccagctatcc
     6301 agcccggcga aggcggcacg cacctgaccg ctgacgaagg attgagtctc gccggcctcg
     6361 ttctgggcca ggctctcgcc aacgcaaacg ataggcgtca ggttgtgagc cagcgcggcc
     6421 ttgatcttct tgttgacacc ctcgtcggtc tcgccaaaca gggcgcgacg ctcggaatgg
     6481 cccagaatca catagcgaca caagccctgg agcatggccg gggagatttc gccggtatag
     6541 gcgccgctga cttcatagta catattctgc gcgccgacgc cgatcttcgt tcccgccagc
     6601 acctgggcga cagcaggaat ggccaggaag ggcgggcaga cggcgatatc aatcccgtca
     6661 atggcgttca gcccgggcgc gatctcgcgc acgaaagccg cagccgcgtc gggggtcttg
     6721 ttcattttcc agtttccggc aacaaatggg gtacgcatgg tttctccatc ttgaattggg
     6781 tattcagccg ttgctcctcg ctgtcgaagg cctggctggt gatttttaat tggaaaggca
     6841 caggggaatg ggcgcggaaa caagcccgca gccgaaacgc ccgtcgcgcc tgctcatttc
     6901 acgcttatga ttatagggga gcggcggcgc ggcggcaatt ttgggcggcc gatattatcc
     6961 acgtcgcaga tattcttccg gcttgatgga gatgacctgg ctggagctgt cgggctgcat
     7021 gctgacgccg tagagcgtct gggcggcctg aacggtgccc cggttatggg taatcaggat
     7081 aaactgggta tgttggccca gctcgcgcag cacgtcgcga aagcggttga cgttggcttc
     7141 gtcgagcgcg gcatccacct cgtcgagtac gcagaagggg ggcggggcga ccttgagcag
     7201 ggagaagata agcgcggcgg ccgtcaatga ccgctcgcct cccgacaaca gcgccagtcc
     7261 ttgctcgcgc cggttgggca gccgggcgat gatctccacg ccgctgctcg tggggtcgtc
     7321 ggggtcggtc atcaccagcc gccccgaccc gccaccgaac agctgctgga aggtctgatc
     7381 gaagatgccg ttgacctcct cgaccgtttt ggagaacgct tttgaggtga gttcatccag
     7441 ttcggcgatc accccgcgca gttgggcgtc cgtctcggtc aggtcgccga cctgttgggt
     7501 caggaattca aagcgctctt cggtggtcgc aagctcggcc ggagcgtcgg ggttaatcgg
     7561 ccccatgcgc tgtaactgcc cgcgatagtg gtggatgttc tcctcaatat cgggcggcag
     7621 ttcgtccacc gccggcaatt cttccacgac ctcgcggatg ggcaggggat gggggccggt
     7681 ctgttcggcg tcgtagctga gcgacaccag gcccaggtcg gcgcggatgc gctcctgcag
     7741 gccctcgatc tggttttcgt gctgcgtctg ccggatgcgc ac
//
LOCUS       DAOMQG010000135         7771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4032_length_7771_cov_9.449974, whole genome shotgun sequence.
ACCESSION   DAOMQG010000135 DAOMQG010000000
VERSION     DAOMQG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4032_length_7771_cov_9.449974"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLC06_08860"
     CDS             complement(<1..769)
                     /locus_tag="PLC06_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HRO90595.1"
                     /translation="MDATGQRELFSGNPDEEPTGPKGASLTREASLQAALGAFERHMQ
                     DEGFSINTVKAFASDIRLLGRYLGIGTPIGEIGVKNLNDFLHWLTHERGVPCSPKSYA
                     RRVTTLKVFFGWLHESGVLAQDPSAAVIQQSVSSPLPEAPRTVDVDRALAVAEAWRLG
                     DQDRKPDARPYLLLTLLLKTGIKKGEAMAIVPNHIDDSDPEHPLLYVRYANPRLRYKE
                     RKLPLDPAWLAVLAEYMSQYQPTDTLFTCTARNLEYIL"
     gene            complement(841..3930)
                     /locus_tag="PLC06_08865"
     CDS             complement(841..3930)
                     /locus_tag="PLC06_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRO90596.1"
                     /translation="MKILRTCGNLTKFLSVLLFLLTVYASGAGRAMAQTTAPVLDLNG
                     EAPGIDFNAVFTEDEGYKAIVSATGLTVAYEGDLTTANVVLTNPLDGNLESLAANPGE
                     TGLSVTYAADKSTLMIKGKGPAASYQQVLRTLTYINSSQSPDITDRVVTVTIGDGQLT
                     SLPATSYIAINAVNDAPVLNTAIALTMAAINEDDQNSNGNSVAGIITLAEAGGQTLIT
                     DVDKNSLEGFAIVEASSLNGVWQYSINGGLSWLPFASVSNTSAVLLDGASRIRFVPNP
                     NFNGSANFVFRAWDQSGGRVSGTTGVDVSVNGGITPYSTATGIVSITVNPINDLPIVD
                     LNGSLPGVDYAVQFFEGGAPVAIAEPGATVTDSDHTQLTRLAITLTNRPDGAAEILAA
                     DVSGTGITPLAYDPLTGMLVLNGPDSISNFQIVLRRVTYANTKSNPTAATRTVTVVAN
                     DGADGPIATSLVSVNPTNSAPVLDPNATISLPPVIEDTAQPAGEMISTILASGGDPIT
                     DPDEAALEGIAIIGADSTKGQWQYSLVNPPTGAADWMPVGVVSDASALLLSDASWLRF
                     VPAPDFSGDAGPATFRAWDRTSGTNGQRVDVSTHGGATAFSVAVNTIDVFVTPVNDPP
                     VLGNLPAGPLLYVEDDAPLPLLGDSLTVTDIDSLSLASATVRLTNPLDGDAEWLLVVT
                     NGTGITANFAGGVLQLTGNAPPLAYQTVLRSVKYWNTSQDPDPTDRVFEVSVFDSQGG
                     RASSTIIVQVQPVNDPPELDLNGSGPGGDYATTFFVKRGAVSVVAEGMTLSDVDNTTI
                     KSATVRIVDIQNGPAEALLADMSGVPNIKLSYVPATGLLTLTGADSVANYARVLRTVT
                     YDNTSSSPAGTVRTIEFILDDGFDTSEPRHTLVSFAEAAPVKLFMPMVSLPSVRAEEP
                     NDKCNEAFGLSLNMNQTFRPDDKDDWFYFDTAQESELTVELRDFLPGVGQILVASEKQ
                     SGQGCNGLVLIGNNGSSNPTKIVSLGRRPAGRYYIWIINDGGYDANSLYRLYVRSTP"
     gene            complement(4035..5735)
                     /locus_tag="PLC06_08870"
     CDS             complement(4035..5735)
                     /locus_tag="PLC06_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HRO90597.1"
                     /translation="MAVASLFEDLLDQYKIEQHVARKQYTDLFQAYDVDDDRQVRIDA
                     LRPEFAGESSFAGRFVSRARALAQVRHPNIAPILHIGKASGGTPYVAQAAVDGYSLTH
                     RLEQLDQRKTPVNPIYACKLIRQLADALILGERLELFHYDLQPDNVLLKNVTLPTDDS
                     VVLIDLFVPAERQPRANGADDHATDKEYLSPEQRAGKDVTAASQVYSLGALLYRMLAG
                     HTPPGPVRNQDTVLNRLFGRPTSLERERGDLTQTTYSVVERSLHKDPRARYQTIEAFA
                     AALDGALAAEESRLGMASGRAPVVERRPMAWLLPLLILALLLTVGGLAMRGFVNRNAA
                     LAGSTPFLANALTGETDNPPMTDTPKPTDEPLPTPTFAASGGQVAGDVTTPTAEPTPT
                     GTAESTPTPTTTETPKPAATEAASATTAPPRVRVMHNLVNLRRGPGVAYALSGTVLGG
                     DLLEVLAWNNDKANPWYLILTGDQRLAWIAATVVQPESAGTLDNVPVAATIPPTPIRV
                     PTSTPTRSVTVIITASPTLDPGFPGGGGETRPPDTSEPSPAAPTLEPTRTPPPFTPSP
                     "
     gene            complement(5881..>7771)
                     /locus_tag="PLC06_08875"
     CDS             complement(5881..>7771)
                     /locus_tag="PLC06_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90598.1"
                     /translation="CDVGSSKRSHILFLASFIALIALLALWGGAGLVSGGTLGIGQTG
                     PLIASSQSPLVQDFFAGDDAHFTITITNTGSIAFDSVVTSGSPVSGCNRNDLGGLNPG
                     QSTSFDCDKTNVNESFMNEIQVTGTAGVTSVSHISKAFVKVLRPQLRITKAPPTQTVS
                     RGGTAYFTIKVWNTWDEVLRVISVDDALVDACDLSPSPSLPVYIEVGDPFTFNCWLSN
                     VQNPFVSIITVTATDPLEAEQYVASDAAWVNMLSLQAGLSAQPASVSEPGDPVTYTVS
                     LTNDGNVPVTLNALTTDRFGNLLSPGNPLIASATNTCLPKPSLPTLPANGGSFQCSFV
                     AHVEGQPSDFITTLTATGKDVDALETTATANASVTIIDVPASMTLTLGADPPFINPPS
                     RQVTFSVRVENTSGADTITITEMTDQFVGNLDGRGTCDLPVTNLPPGFSYQCEFSAVV
                     SGTIGQQKSRIVTVNAVDDDLPPGDLSVNGIVTVGITDQPTQYAYMPNVNEVTAKRTS
                     CGRPFPLVNNQQYQFQPPNTYDSNLPVANRDQHYFVFELTQSSSVKVELTNFVPRKGQ
                     LIVRPHTEGGNPPCGDSIGRNTSEGLNKTVNLGNLAAGKYYIQLINDGPSDVGDFYGL
                     IVRVQ"
BASE COUNT         1493 a         2238 c         2478 g         1562 t
ORIGIN      
        1 ggaggatgta ctccagattg cgagccgtgc aggtgaacag cgtgtcggtg ggctgatatt
       61 ggctcatgta ctcggccagc acggcaagcc aggccgggtc gagcggcaac ttgcgctcct
      121 tgtagcgcaa gcgcggattg gcataacgca cgtagagcag aggatgctcc gggtcgctat
      181 cgtcgatatg gttgggcacg atggccatgg cctccccttt cttgatgccg gtcttcagca
      241 aaagagtcaa caatagatag gggcgggcgt cgggcttgcg atcctgatcg ccaagccgcc
      301 acgcctcggc tacggccaag gcccgatcga catccacggt gcgaggagct tccggcaagg
      361 ggctgctgac gctctgctga ataaccgccg ccgatgggtc ctgggccagt acgccgctct
      421 catggagcca tccgaagaac accttcagcg tcgtcacccg gcgggcgtag gatttggggc
      481 tgcagggcac gccccgctcg tgggtcagcc aatggagaaa atcgttgagg ttcttgacgc
      541 cgatctcgcc gatgggtgtg ccgattccca gatagcggcc gagcagacga atgtcgctgg
      601 cgaaggcctt caccgtgttg atggagaacc cttcatcctg catgtgtcgt tcaaaagcgc
      661 ccagggcggc ctgcaaagac gcctcgcggg tcagcgacgc gcctttgggg ccggttggtt
      721 cttcatcggg attgcccgaa aacagttctc gttgtcctgt tgcgtccatg atcgcttcct
      781 gtttgatatt cgtacagtta tgcgaaatta tagaacaggt gggcgatcct gtcaagccct
      841 ttagggcgta gagcggacat acaggcgata gagcgagttt gcgtcgtacc cgccgtcgtt
      901 gatgatccag atatagtatc ggcctgccgg acgtcgtccc aatgagacga ttttggtggg
      961 gttggaagag ccattgttac ctatcaggac caggccattg cagccctggc ctgattgttt
     1021 ttccgacgcc acaaggatct gaccaacgcc cggcagaaaa tcgcgcaatt caactgttaa
     1081 ttcactctcc tgagctgtgt cgaaatagaa ccagtcatcc ttgtcatcgg ggcggaaggt
     1141 ttgattcatg ttgagcgaca ggccaaaggc ttcattacac ttatcgttag gctcctcggc
     1201 gcgaacgctg ggcagggaga ccatcggcat aaacagtttg accggggccg cctcggcgaa
     1261 cgaaaccagc gtatggcgcg gctcgcttgt gtcaaaaccg tcgtcgagaa taaactcgat
     1321 cgtccgcacg gttccggccg gtgacgacga cgtattgtcg taggtgacgg tgcgaagcac
     1381 acgagcgtag ttggcaacgc tatccgctcc cgtcagcgtc agcagtccgg tcgccgggac
     1441 ataagagagc ttgatattgg ggaccccgct catgtcggcc aacaaagctt ccgccgggcc
     1501 attctgtata tcgacgatac gaaccgtggc tgatttgatc gtcgtgttat ccacgtcact
     1561 cagcgtcatg ccctcggcaa ccacggatac ggcgccgcgc ttgacgaaaa acgtcgtcgc
     1621 gtaatctcca ccggggccgg agccattgag gtccagctca ggcggatcgt tcaccggctg
     1681 aacctggacg ataatcgtag acgatgcgcg ccccccttga ctgtcaaaca cggaaacttc
     1741 gaatacccga tcggtaggat cggggtcttg tgaggtattc cagtatttaa cgctgcgcaa
     1801 gaccgtctgg tatgccaatg gaggcgcatt gccggtcaat tgcagcacgc cccccgcaaa
     1861 gttggccgta atcccggtgc cattcgtcac gaccaacagc cactcggcgt caccatccag
     1921 cgggttggtc aggcgcacgg tggcggaagc aagcgacagg ctatcgatgt ccgtcaccgt
     1981 cagcgaatcg cccagcaacg gcagcggagc gtcgtcctcg acatacagca aggggccggc
     2041 cggcaagttg cccaacacgg gcggatcgtt cacgggcgtg acaaatacgt cgatcgtatt
     2101 gacagcaaca ctgaacgctg tggcgccccc gtgagttgac acatcgacgc gctggccgtt
     2161 tgtgccgctg gtgcgatccc aggcgcggaa tgtggccggg ccggcatcac cgctgaaatc
     2221 gggggcgggc acaaagcgca gccatgaagc atcgctcagc aataaggcac tggcatcgga
     2281 tacaaccccc accggcatcc agtcggccgc gccggttggt gggttgacca gggaatattg
     2341 ccattgcccc ttggtcgaat ccgcgccgat gatggcgata ccttccagcg ccgcttcgtc
     2401 gggatcggtg atcgggtcac cgccggaagc gaggatcgtt gaaatcattt cgcctgcggg
     2461 ctgtgccgta tcctcgatga cgggcggcag ggatatcgtg gcgttcgggt caaggaccgg
     2521 cgcgctgttg gtcgggttca cactgacaag cgacgtggcg atcgggccgt cggctccgtc
     2581 gttggccacg accgtcaccg tgcgcgtggc tgccgtcggg ttgcttttgg tgttggcgta
     2641 ggtgacgcgc cgcaggacga tctggaagtt gctgattgag tcggggccgt ttaagaccag
     2701 catgccggtc agtggatcat aggcaagcgg cgtgatgccg gtgccgctca catcggcggc
     2761 caggatctcg gccgcgccgt cgggtcgatt ggtcagcgtg atggccaacc tggttagctg
     2821 ggtatgatcg ctatcggtta cggtcgcgcc aggctcggca atggcgacgg gcgcgccacc
     2881 ctcaaagaat tgaaccgcat aatcgacacc cggcaggctg ccgttcaggt caacaatcgg
     2941 caggtcattg atggggttga ccgtgatcga gacgatgccg gtcgcggtgg aatagggggt
     3001 gatgccgcca ttgaccgata catcaacgcc ggtcgtgccg ctcacccggc cgcccgactg
     3061 atcccaggcg cgaaacacga agttggccga gccgttgaag ttggggttgg gcacgaagcg
     3121 gattcgcgac gcgccatcca acaggacagc cgaggtgttc gatacgctcg cgaacggcag
     3181 ccagctaagc cctccattga tggaatattg ccagacacca ttgagggatg aggcctcgac
     3241 gatagcaaag ccctcgaggc tgttcttgtc gacgtcggtt atcagggttt gcccaccggc
     3301 ctcggccagc gtgataatcc ccgccaccga attgccgttc gaattctggt cgtcctcgtt
     3361 gatggctgcc atcgttaagg caatggcggt attgagcacc ggcgcatcgt tcacggcgtt
     3421 gatcgcgatg tagctggtcg ccggcaggct ggtcagctgc ccatcgccga ttgtcacggt
     3481 gacgacgcga tcggtgatat ccggcgattg tgacgaattg atgtaggtca gcgttcgcaa
     3541 tacctgctga tagctcgcgg cggggccttt gcccttgatc atcagcgtgc ttttatcggc
     3601 ggcataggtg acgctcaggc cggtctcgcc gggatttgcc gccagcgatt ccaaattgcc
     3661 gtcgagaggg ttggtcaaca ccacgttggc cgtggtcaga tcgccttcat aggcgacagt
     3721 caggccggtc gcgctgacga tggccttata gccttcgtct tcggtgaaaa cggcgttgaa
     3781 atcgatgccc ggggcctcgc cgttcaggtc cagcacaggg gcagtcgtct gcgccatggc
     3841 acggccggct ccggacgcat agaccgtgag caggaacaac aataccgaca aaaacttcgt
     3901 caaattgcca caggttctca aaatcttcat atcgttacat gctcactatt ccgtatcccg
     3961 gctctttccc cccgggttcg gagtgaatag tcaagcttca aaacgctgcg acctgcccgg
     4021 cggccgcaga gccatcatgg agacggcgta aacggcggtg gcgtcctggt aggctccagg
     4081 gtcggcgccg ccggtgacgg ctcggacgtg tccggcggcc gggtttcccc accacccccg
     4141 ggaaagcccg ggtcgagcgt cggcgaagcg gtgatgatta cggtcaccga tcgcgtcggt
     4201 gtcgatgtcg gcacgcgaat gggcgtcggc ggaatggttg ccgccacggg cacgttatcc
     4261 agcgtccccg ccgactcagg ctgcacgacc gtcgccgcga tccaggcgag ccgctggtcg
     4321 ccggtgagta tcagatacca ggggttggcc ttgtcgttat tccaggccaa cacttccagc
     4381 agatccccgc ccaggaccgt gccggacagg gcatatgcga ccccggggcc acggcgcagg
     4441 ttcaccagat tgtgcatcac ccgcacacgt ggcggcgcgg ttgtggccga ggccgcttcc
     4501 gtcgccgcag gcttcggggt ctccgtcgtg gtgggcgtgg gggtcgattc ggccgtgccg
     4561 gtgggtgtcg gctccgccgt gggagtggtg acatctcccg caacctggcc gccgctcgcg
     4621 gcaaaggtcg gcgtcggcaa tggttcatca gtgggtttcg gcgtgtccgt catcggcgga
     4681 ttatcggttt cgccggtcag ggcattggcc aaaaagggag ttgacccggc cagagccgca
     4741 ttgcgattca caaatccgcg catggccagc ccgcccacag tcagcagcag cgccaatatg
     4801 aggagcggca ataaccaggc catcggccgc cgttcgacga ccggggcgcg gccgctcgcc
     4861 atccccagcc gggattcttc ggccgccagc gcgccatcga gcgccgcagc aaacgcctca
     4921 atagtctgat agcgagcgcg ggggtccttg tgcagggaac gttcgacgac gctgtaggtc
     4981 gtctgggtca aatcgcctcg ctcgcgttcg agggaagtgg ggcggccgaa cagacggttg
     5041 agcacggtat cctggttccg cacaggtccg ggcggcgtgt gccccgccag catgcgatag
     5101 agcaatgcgc ccaacgagta aacctggctc gccgcggtga catccttgcc ggcccgctgc
     5161 tcgggcgaaa gatattcctt atctgtcgca tgatcgtcag caccattggc gcgcggctgt
     5221 cgttcggcgg gcacaaacag gtcgatgagg acgacggaat catccgtggg cagggtcaca
     5281 tttttcagca acacattatc aggttgcagg tcgtagtgga aaagctcgag gcgttcgccc
     5341 agtatcagcg cgtcggccag ctggcgaatc agcttgcagg catagatcgg gttgaccggc
     5401 gtcttgcgct ggtcgagctg ttccagccgg tgggtcagcg agtagccgtc gaccgcggct
     5461 tgggctacat agggtgtgcc gccggatgcc ttgccgatgt ggaggatggg ggcgatattg
     5521 gggtggcgca cctgggccag cgcccgcgcg cgactgacaa accggccggc aaacgagctt
     5581 tccccggcga attcggggcg cagggcgtcg atccgcacct ggcggtcgtc atcaacatca
     5641 tacgcctgga acagatcggt atactgcttc cgggcaacgt gctgttctat tttgtattga
     5701 tccagaagat cttcgaacaa gctcgctact gccatttgaa ttccgcgcgt ttgcctttcg
     5761 gctcaatcca actctgaaat cattcgcttc gcccggctcc cgatgggcac atgaaattct
     5821 acccggccgg tatctgtgta atcattctag tcccgataga taaaggataa gcttaaggta
     5881 ctattgaacc cgaacaatca ggccatagaa atcgccgacg tccgatggcc catcgttgat
     5941 gagctggatg taatattttc cggcggcaag gttacccaga ttaaccgtct tgttaagccc
     6001 ttcgctggtg tttcgaccga tagaatcgcc gcaaggcggg ttcccgcctt ccgtgtgcgg
     6061 gcgaacgata agctgcccct tgcgtggcac aaagttggtc agttccacct tcaccgaact
     6121 gctctgcgtc agctcaaaca cgaagtagtg ctgatcccga ttcgccaccg gaagattcga
     6181 gtcataagtg ttgggcggct ggaattggta ctgctggttg ttgaccagcg ggaaggggcg
     6241 gccgcagctc gttcgcttgg cggtgacctc gttgacgttg ggcatgtagg cgtattgcgt
     6301 cggctggtcg gtaatcccaa cggtgacaat tccattcacg ctcaggtcgc ccggcggcag
     6361 gtcgtcgtcc acggcattga cggtgacgat gcgcgacttc tgctgcccga ttgtgcccga
     6421 gacgacggcc gaaaactcgc attgatacga aaagcccggg ggcagattgg tcaccggcag
     6481 atcgcatgtg ccgcggccgt ccagattgcc cacgaactgg tcggtcatct cggtgatcgt
     6541 gatcgtgtcc gcgccgctcg tgttttcaac gcgaacgctg aacgtcacct ggcgactggg
     6601 cgggttgatg aagggcggat cggcccccag cgtgagtgtc atcgaggccg gaacgtcgat
     6661 gatggtcacc gaggcattgg cggtggctgt ggtctcgaga gcatcgacat ctttgccggt
     6721 cgcagtcagt gtcgtgataa agtcggatgg ctgtccttcg acatgggcca cgaagctgca
     6781 ttggaacgac ccgccattag ccggcaatgt cggcaaggag ggcttcggca agcaggtgtt
     6841 ggtcgctgaa gcgatcagcg gattgccggg gcttaggagg ttgccgaacc ggtcggtggt
     6901 cagggcattg agtgtgaccg gcacattgcc gtcattagtc agggaaacgg tataggtaac
     6961 ggggtcgccc ggttcggaga cggacgcagg ttgggcgctc agcccggcct gcaaagacag
     7021 catattgacc caggccgcgt cgctggcaac gtattgctcc gcttccaggg ggtcggtcgc
     7081 tgtcacagtg atgatcgaca caaaagggtt ctgaacattg ctaagccaac aattaaaggt
     7141 gaaggggtct ccgacttcga tatacacggg gagtgacggc gacggcgaca gatcgcaagc
     7201 gtcgaccagt gcgtcatcga ctgaaatgac ccggagcacc tcgtcccagg tattccaaac
     7261 cttgatcgtg aagtaggcgg ttcccccgcg agagacggtt tgagtcggag gggctttggt
     7321 gatgcgcaat tgaggcctca gcaccttgac gaacgccttt gagatgtggc tgaccgatgt
     7381 cacgccggcc gtgccggtca cctggatttc gttcatgaag ctttcgttga cgttggtttt
     7441 gtcgcagtca aacgacgtgc tttggccggg gttcagcccg ccgagatcgt tgcgattgca
     7501 ccccgacacc ggcgaacctg atgtgacgac cgaatcgaag gcaatgctgc ctgtgttggt
     7561 gatggtgatg gtgaagtggg cgtcatcgcc ggcaaaaaag tcttgcacca gcgggctttg
     7621 ggaggaggcg atcagcgggc cggtctgccc gatccccaac gtgccgccgg agaccagtcc
     7681 cgcgccgccc cacagggcaa gcagcgcgat cagggcgatg aaacttgcca gaaacagtat
     7741 gtgtgatcgt ttcgaacttc ccacgtcaca t
//
LOCUS       DAOMQG010000136         7700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4080_length_7700_cov_11.214519, whole genome shotgun sequence.
ACCESSION   DAOMQG010000136 DAOMQG010000000
VERSION     DAOMQG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4080_length_7700_cov_11.214519"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..301
                     /locus_tag="PLC06_08880"
                     /pseudo
     CDS             11..301
                     /locus_tag="PLC06_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            313..2484
                     /locus_tag="PLC06_08885"
     CDS             313..2484
                     /locus_tag="PLC06_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRO90599.1"
                     /translation="MAYFLIRMAIYTTAAAIVMNVVPGLRLDPYPYLGEPFTTIGAYI
                     MIGLIFGTLHTFVRPIILFLTGRLYIWSMGLVALVIDVFIFLLLSYLAPTVWQVGGAR
                     LLPATLGAMLMGVIVLGLEALFGFDSPRVNEVNKSAFYWRWLGMLPTGQRNRLVENLR
                     TQQMVNTIQSYAIDTLVGLSPFRGFRRAMQRILYRDRPRLMEDNPAVKLRLMLQELGP
                     TFVKFGQMAAGRVEMLPVAWQAELEKLQDDVKPFAYAEVEQIIRRELGAPPDQVFTTF
                     DQHPLAAASTGQVHAATLHSGEAVIVKVRRPNVEVTVKGDLNVMQDVIDTAERRLSWL
                     RRFGLSALFHEFSGNILIELDYTNEAYHAHLLRHKMQKLPDIHIPMIYDAWSTTSVLT
                     QERVTGVKITDVTAIDAAGIEREETALNFFRALLQQLLFDGFFHADPHPGNVWVDLRT
                     GQVIFLDMGMMGHLSRADRLVLGRLIWALQDRDARMVARNMVAMCAPTKGHDSAALEY
                     DVERLINRHLLLSSTPPEITAIINDMVGLLMRHDLRLRKEFTLALKAMGQGETIMRVL
                     MGDKSPDAILDITYTTMRALLLAQLRSQDLVAAGKPLAREVADRLPELLIATSALLDD
                     FQRGRTPLLPGQREIDELTDDLQQNLRQGVRRIVLSVLLVGLLVGSSLMLLAPIEGRV
                     SEYEALAVRLVAVSGFVISTAAILIMLLVVLMGTLKQQDDG"
     gene            2686..3048
                     /locus_tag="PLC06_08890"
     CDS             2686..3048
                     /locus_tag="PLC06_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRO90600.1"
                     /translation="MSTILSLVVSWLLAALVIYIVGRLNLGLTVDSFVSAIIAAAVIA
                     IITWVVMWLLTDVLKISIGAAWFGAVVSLVISALILLFSERFVKGMKVAGFNGAIIAA
                     IAIAVVTWLGDWLVTSLF"
     gene            3287..4402
                     /locus_tag="PLC06_08895"
     CDS             3287..4402
                     /locus_tag="PLC06_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRO90601.1"
                     /translation="MSNVRVLVGTRKGAFILTADEKRDKWHVEGPLFGGLEIYHLKGS
                     PVDPNRIYASQASDWFGQVIHRSDDGGKTWTTVGNEFAYDGPTGTHQWYDGTAHPWDF
                     RRVWHLEPSLHDPDVVYAGVEDAAIFRSDNGGRTWQELPGLRRHDTSGIWQTGGGGFC
                     LHTIILDPTDPDRIYIAISAAGAFRTDDGGQTWKPINRGLHSDYIPDANAEVGHCVHR
                     IAMHPSQPNVLFMQKHWDVLRSDNAGDDWHEVSGNLPSDFGFVVDVHAHEPETIYVVP
                     IKSDSEHYPPEGKLRVYRSRTGGNEWEALTKGLPQEDCYVNVLRDAMAVDNLDSCGVY
                     FGTTGGQVYASADAGDNWNAIVHDLPAVLSVEVQTLP"
     gene            4399..4695
                     /locus_tag="PLC06_08900"
     CDS             4399..4695
                     /locus_tag="PLC06_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRO90602.1"
                     /translation="MIRVIIPHHLRNLAGTGAEVTVEVAGPVTQRAVLDALEARYPVL
                     RGTIRDYATGQRRPFLRFFACTEDLSHESPDALLPEEVAAGIEPYRIIGAIAGG"
     gene            4953..5570
                     /locus_tag="PLC06_08905"
     CDS             4953..5570
                     /locus_tag="PLC06_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90603.1"
                     /translation="MSDMVFGAIGLIIFLALIFVAGALLYKLVNARFNRAWGPLMPLI
                     NGKVVGDGGGGATSWLVGAYQGRKVQASMTPGRNMYSPAGSSGGKYNYFEVALADIGG
                     KRDWSVTFDRKILGIGQTGWRVKSDDPSVEASLQAADVISLLTPFGEPAGHFNLAPLD
                     YNHRERLLCFRYDNGAQWAPTPQSFAAQLDMLVRAAEINERVNPA"
     gene            5595..6956
                     /locus_tag="PLC06_08910"
     CDS             5595..6956
                     /locus_tag="PLC06_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016975824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRO90604.1"
                     /translation="MMETPTADPSFEVHDPAILAQARSILDACRNGREMQDAVIAAVR
                     RNERWRGEQCINLLAPEAPTSPAVRALLSSEVGTRAAEGHIGRVNRWFAGTAYIDEIE
                     ALCVELLKQAFRARYADHRLVASMIGNLAVYTALTEPGDVIMSIAQPFGGHSSNRPDG
                     PAGVRGLKIVDIPMDPVELEIDLDLFRKTAPLVRPKLVALGASMTLFPFPLQEMSDTL
                     AEWGGKLFFDGAHQIGLVAGGQFQDPLREGAAVMTGSAGKTFSGPQSGIIVWDDPTIT
                     ESITHAIFPVLAATHQVNRVAALAVAAAEMIAFGEVYMAQIVRNAQALGAALERRGIR
                     VLGAHKGYTRTHQVIGDARAFGGGLDTAHLLARANLITNKNLIPGDTPEDWDRPGGLR
                     MGTIEVTRLGMHEPEMDTIAGFMARVLVEREAPESVMEDVIDFRQGYQTLYYDFDNGL
                     PAR"
     gene            6953..7534
                     /locus_tag="PLC06_08915"
     CDS             6953..7534
                     /locus_tag="PLC06_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRO90605.1"
                     /translation="MSKSRLEAFSDGVIAILITIMVLELRPPEGAELGALRPLIPVFL
                     SYILSFIYVGIYWNNHHHLFQVVERVDGRVLWANLHLLFWLSLIPFATAWLDEAGLTP
                     WPAAFYGMVLLMAAVAYFILLRALLTIHPPDSRLAQAVGRDVKGKLSVVIYLVAIVLA
                     FIAPWLSVALYVLAALIWFVPDRRMEKTLVDAH"
BASE COUNT         1467 a         2339 c         2349 g         1545 t
ORIGIN      
        1 atagcatggc atgggttgtg ctatgatcgc cgtcatgtcg gcgcctaatc cctatcaacc
       61 cgtccccgaa gtccgaatca cccatcccga gtggagcaaa agcgccacta tctaccagat
      121 caacacccgc cagttcactc cggaaggcac actgcgcgcg gccgagacga tgaagtacca
      181 ggtgcgcgag gccgatatcg acggctaccg ctgcgacgtg gtcgggttcg tgcccaccga
      241 tttccggaac gacgtccgca aggaactcga tgccatcagg ccggtcttca tgttggccga
      301 gtgagataac ccatggccta ctttctcatt cgaatggcga tctacacgac ggccgcggcc
      361 atcgtgatga acgtcgtgcc ggggttgcgc ctcgatcctt atccctatct gggcgaaccg
      421 ttcacgacga tcggcgccta catcatgatc ggcctgatat tcggcacatt gcacaccttt
      481 gtccgtccca tcatcctctt cctgaccggc cggctctata tctggtcgat gggcctcgtg
      541 gccctcgtaa tcgatgtctt catcttcctg ctcctatcct acctcgcgcc caccgtctgg
      601 caagtcggcg gagcgcgatt gttgccggcg acattgggcg cgatgttgat gggcgttatc
      661 gtgctcggcc tggaggccct cttcggcttc gacagcccgc gggttaacga ggtgaacaag
      721 tcagccttct actggcgctg gctgggtatg ctgccgacgg ggcagcgcaa tcgactggtc
      781 gaaaacctgc gcacccagca aatggtcaat accatccaga gttacgccat cgacacattg
      841 gtgggcctct cgccgtttcg cgggttccgg cgcgccatgc aaaggatact ctatcgcgac
      901 cgaccccgat tgatggaaga taacccggcc gtcaagctgc gactgatgtt gcaggagctt
      961 ggccccacat tcgtcaagtt tgggcagatg gccgcgggcc gcgtggagat gctgcctgtc
     1021 gcctggcaag ccgaactgga gaagctgcaa gatgacgtga agccattcgc ctatgccgag
     1081 gttgagcaga tcattcggcg cgaactggga gcaccgcccg accaggtttt cactacattc
     1141 gatcaacacc cgctggcggc cgcttcaacg gggcaggttc atgccgcaac actccacagc
     1201 ggcgaggcgg ttatcgtcaa ggtacgacgt cccaatgtcg aggtcacggt gaagggcgat
     1261 ctcaacgtca tgcaagacgt gatcgacacg gccgaacgac gcctgtcctg gcttcgtcgg
     1321 ttcggtctca gcgcattgtt ccacgaattc tccgggaaca tcctgatcga gctggattac
     1381 accaacgaag cctatcacgc ccatcttttg cgccacaaga tgcagaagct gccggatatt
     1441 catatcccga tgatttatga cgcctggagc acaacgagcg tgttgacgca ggaacgcgta
     1501 acgggggtaa aaatcacgga cgtgacagcc atcgacgcgg ccggcatcga acgcgaagaa
     1561 acggcgctca actttttccg ggcgctgctc cagcagttat tgttcgacgg cttcttccat
     1621 gccgatccgc atcccggcaa cgtatgggtc gatcttcgaa ccggtcaggt catctttctc
     1681 gacatgggca tgatgggcca tctgagccga gccgaccggc ttgtgttggg gaggttaata
     1741 tgggccttgc aggatcgcga tgcccggatg gtggcacgga atatggtcgc catgtgcgca
     1801 ccgaccaaag ggcacgattc ggccgcgctg gaatacgacg tcgagcgatt gatcaaccgc
     1861 catctgcttc tgtcgagcac gccacccgaa ataacagcga tcatcaacga catggtcggc
     1921 ctgctcatgc gccatgacct gcggctgcgc aaggagttca ccctggcgct caaggcaatg
     1981 ggccagggcg agacaatcat gcgcgtcctc atgggagaca agtcgccgga cgccattctc
     2041 gacatcacct atacgacgat gagagcgttg ctcctcgctc aactcaggtc acaggattta
     2101 gttgctgcgg ggaaaccact ggcacgcgaa gttgccgatc gcctgccgga actgctgatc
     2161 gccacgtctg ccctgctgga tgactttcag cgcggccgaa cgcctctgtt gcccggccag
     2221 agggagatcg acgaactgac ggatgacctg caacaaaact tgaggcaagg tgtgcgccgg
     2281 atcgtcctga gtgtattgct cgtcggtctt ctagtcggtt cgtcgctcat gctcctcgcc
     2341 ccaatcgaag gcagggttag cgagtatgaa gcccttgccg tgcgccttgt cgcggtttcc
     2401 ggattcgtga tcagcaccgc ggcgatcctg atcatgttgc tggttgttct gatgggcacg
     2461 ctgaagcagc aggatgatgg atgatgcaag agggtcgagg gaaagacacg atacgattgt
     2521 gtatgccgga tcgtaaccac catggcacat cagtgttgtt aatgatagga tttctgcgtt
     2581 caagggatgc ggcaagtcca tcccgcggca ggcggattac aaacccgacc gatttggcgg
     2641 gtaatctttg tttgaattat tgttcatgct tggaggatag gtgctatgag tacaattctg
     2701 agtctcgttg tttcctggtt actggccgct ctggtcatct acattgtggg acggctcaac
     2761 cttgggttga cggttgacag cttcgtcagc gccatcatcg ccgcggcggt catcgccatt
     2821 atcacctggg tggtcatgtg gctgttgacg gatgtcctga agatcagcat cggcgccgcg
     2881 tggttcgggg ccgttgtctc cctggtcatt tcggctctca tcctgttatt cagcgaacgc
     2941 ttcgtcaagg ggatgaaggt tgccggtttc aatggcgcca tcatcgccgc catcgccatc
     3001 gccgtcgtca cctggctggg cgattggctg gtcacctccc tgttctaata accgggcgac
     3061 ggttgatctg attccggatt aaaatggctt gcagaaaaac gcccggcctc gcggtcgggc
     3121 gttttttatt tgcccatggc tattgcgtct cccttcgggg taactcccag ctcaaatcgg
     3181 cgattgacaa tacagaatac atgttctatg attgcagcgt tcgttggaca aagaacgaca
     3241 gttgaatatc ggggatgccg ctaaacgtct ttcataggag aacacaatga gcaacgtaag
     3301 agtattggtt ggcacacgca agggcgcatt cattctgacc gccgacgaaa agcgtgacaa
     3361 atggcacgtt gaaggccccc tctttggcgg actggagatc tatcacctca aaggctcccc
     3421 ggttgacccg aatcgcatct atgcctcgca ggccagcgac tggttcggcc aggttatcca
     3481 ccgttccgac gacggcggca agacgtggac gaccgtgggc aatgagttcg cctacgacgg
     3541 cccgacaggc acgcatcaat ggtacgacgg cacggcgcac ccttgggatt ttaggcgcgt
     3601 ctggcacctg gagccgtcgc ttcacgaccc cgacgtggtt tacgccgggg tagaggacgc
     3661 ggccatcttc cgcagtgaca acggcgggcg gacctggcag gagttgcccg gcttgcgccg
     3721 tcacgacacg agcggtatct ggcaaacggg cgggggcggt ttttgcctgc acaccatcat
     3781 cctcgacccg acagaccccg accgcatcta catcgccatc tcggctgccg gggccttccg
     3841 caccgacgac ggcggccaaa catggaaacc catcaatcgc ggtctgcact ccgactatat
     3901 ccccgatgcc aacgccgagg taggccactg tgtccaccgt atcgccatgc acccgtcgca
     3961 gcccaacgtg ctgttcatgc agaagcattg ggacgtgctg cgcagcgaca acgctggcga
     4021 cgactggcac gaggtcagcg gcaatctgcc cagtgatttc ggcttcgtcg tcgacgtcca
     4081 cgcccacgag ccggagacga tctacgtcgt gcccatcaag agcgactcgg agcactaccc
     4141 gccggagggc aagttgcgcg tctatcgcag ccgcaccggc ggcaacgagt gggaggcgct
     4201 gaccaaggga ctgccgcagg aggattgcta cgtcaacgta ctgcgtgacg cgatggctgt
     4261 ggataatctc gattcctgcg gcgtctactt cggcacgacc ggcggccagg tctacgcctc
     4321 ggccgacgcc ggggataact ggaacgccat cgtccacgac ctgccggccg tcctgtcggt
     4381 cgaggtgcag acactgccgt gatccgcgtc atcattcccc accacctgcg taatctggcg
     4441 ggcacgggcg cggaagtgac ggtcgaggtg gccgggccgg tcacgcagcg ggcggtgctc
     4501 gacgcgctgg aggcccgcta cccggtgctg cgcgggacga ttcgcgacta cgccaccggg
     4561 cagcggcggc cgttcctgcg cttcttcgct tgcacggaag acctgtcgca tgagtcgccg
     4621 gatgcgttgc tgccggaaga agtcgccgcg gggatagaac cctatcgcat catcggagca
     4681 attgccggag ggtagaccct tctcgcccgt accatcaagc agctgacagg cggccgatcc
     4741 tggatgaatg gccgggtcat ctggcgaagc gtcaaacacc cacgaggtca tgtaaggccc
     4801 aggacgagtg caccagccag ggcccaggtt cgacgattca gccaatcgcc gggcctgggc
     4861 tcacaacgat cacgcctgat tgacatcact gctcatcgcc gtgtatgcta tcgattggcg
     4921 acaatcctga gaaccggaga gaaaacgcat ccatgtctga tatggtgttc ggcgcaatcg
     4981 gtctgatcat atttctggcc cttatctttg ttgcgggcgc gctcttatac aagctcgtta
     5041 acgcgcggtt taatagagcc tggggcccct tgatgcctct catcaacggc aaggtcgtcg
     5101 gcgacggcgg cggtggcgct accagttggc tcgtgggagc ctatcaggga cggaaggtgc
     5161 aagccagcat gacccccggc cgcaacatgt attcgccggc tggctcgagc ggtggaaaat
     5221 acaactactt tgaggtcgct ctggctgata tcggaggcaa gcgtgactgg agtgtcacct
     5281 ttgatcgcaa gattctcggc atagggcaaa ccggctggcg ggtaaagtcg gatgaccctt
     5341 cggtcgaggc atcgttgcag gctgccgatg taatctcgtt gttaacccct ttcggagagc
     5401 cggccggcca tttcaacctc gccccgctgg attataacca tcgcgaaagg ctcctttgct
     5461 ttcgatatga caacggggcg cagtgggcac cgacaccgca gtcctttgca gcgcagcttg
     5521 atatgctggt tcgggcagcg gagatcaatg agcgcgtgaa cccggcttaa aattaccaac
     5581 gttatagagg caacatgatg gaaacaccta cggccgatcc ttcctttgaa gtccacgacc
     5641 ccgccatcct ggcccaggcc cgctcgatcc tcgatgcctg ccgcaacggc cgcgagatgc
     5701 aggacgccgt catcgccgcc gttcggcgca acgagcgctg gcgtggcgag caatgcatca
     5761 acctgctggc ccccgaggcg ccgaccagcc cggccgtgcg ggcgctgctg tcgtcggaag
     5821 tgggcacgcg ggcagccgag ggccacatcg gccgcgtcaa tcgctggttc gccggcacgg
     5881 cctacatcga cgagatcgag gcgctgtgcg tggaattgct gaagcaggcc tttcgcgccc
     5941 gctatgccga tcatcgtctg gtggcgagca tgatcggcaa cctggccgtc tacaccgccc
     6001 tgaccgagcc gggtgacgtc atcatgagca tcgcccagcc tttcggcggc cactccagca
     6061 accggcccga cggcccggcg ggggtgcgcg ggttgaagat cgtcgatatt cccatggatc
     6121 cggtcgaact ggagatcgat ctcgacctgt ttcgcaagac cgcgccgctg gtgcggccga
     6181 agctggtggc gttgggcgca tcgatgacgc tcttcccctt ccccctgcaa gagatgagcg
     6241 acaccctggc cgagtggggc ggcaagctgt tcttcgacgg cgcgcaccag atcggcctcg
     6301 tcgccggggg tcagttccag gacccgctgc gcgaaggcgc ggcggtcatg accggctcgg
     6361 cgggcaagac cttcagcggc ccgcagagcg gcatcatcgt ctgggacgat ccgacgataa
     6421 ccgaatcgat cacccacgcc atcttccccg tgctggccgc cacgcatcag gtcaaccgcg
     6481 tcgccgcgct ggccgtggcc gcggccgaga tgatcgcctt cggtgaggtg tacatggccc
     6541 agatcgtgcg caatgcccag gcactgggtg cggcgctgga gcggcggggc atccgcgtgc
     6601 tgggggcaca caagggctac acgcgcaccc atcaggtcat cggtgacgcg cgggccttcg
     6661 gcggcggtct cgacacggcc catctgctgg cacgggccaa cctgatcacc aacaaaaacc
     6721 tcatccccgg cgacacgccc gaagactggg atcggccggg cggcctgcgc atgggcacca
     6781 ttgaggtcac ccggctaggg atgcacgagc cggagatgga caccatcgcc gggttcatgg
     6841 cgcgcgtgct tgtcgagcgc gaagcgccgg aatcggtgat ggaggatgtg atcgacttcc
     6901 gacaggggta tcagaccctc tattacgact ttgacaatgg acttccggcg cgatgagcaa
     6961 gagccgcctt gaagccttca gtgacggcgt catcgccatc ctcatcacga tcatggtgct
     7021 ggaactgcgc ccacccgaag gcgcggagct gggcgctctg cggccgctca ttcccgtctt
     7081 tctcagctac atattgagct ttatctacgt gggcatctac tggaacaacc accaccatct
     7141 gttccaggtt gtggagcggg tcgacggtcg tgtgctctgg gccaatttgc acctgctgtt
     7201 ctggctgtcg cttatcccct tcgccacggc gtggctggat gaggccgggt tgacgccctg
     7261 gccggcggcg ttctacggca tggtgttgct catggcggcc gtggcctatt tcatcttgct
     7321 ccgcgcgctg ctgaccatcc atccccccga ttcaaggctg gcgcaggcag tcgggcggga
     7381 cgtcaagggc aagctgtcgg tggtcatcta tctggtcgcc atcgtgctgg ctttcatcgc
     7441 cccgtggttg tcggtggcgc tctatgtgct ggcggcgctt atctggttcg tgcctgacag
     7501 acggatggag aaaacattag tggatgccca ttagaatgca ttcgcgagag aatggcgtta
     7561 tggatattta tttggagaca ggccaaaagc gaacgttggc cgtggcactt gattggccgg
     7621 gctgggcacg cggcggccgt gacgagggca cagcgcttca gacgctgctc gactacgccc
     7681 cgcgctatgc ggccgtggcc
//
LOCUS       DAOMQG010000137         7696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4084_length_7696_cov_9.721110, whole genome shotgun sequence.
ACCESSION   DAOMQG010000137 DAOMQG010000000
VERSION     DAOMQG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_7696_cov_9.721110"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..754
                     /locus_tag="PLC06_08920"
     CDS             5..754
                     /locus_tag="PLC06_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRO90606.1"
                     /translation="MRRSMHPPQQNKENQMTQVETPAVAPRAAAVTFSRAAIIAVAAY
                     AGAQMLSDIASLKIGVVAGFAVDMGTFIYPITFTLRDVAHKILGKRNVRTLIVMAAVI
                     NLVMAAYLAWAATVPSDPSWGLGDSFSAILGPVWRIVIASILAELVSELVDTEIYHWF
                     VTKVTTRYQWARVLSSNSISIPIDNFIFAVGAFGPLPGLENSPLSLPWPVVWQIFLFN
                     LVVKYLVTLISLPLIYTTPDADWSRREESDR"
     gene            complement(751..1806)
                     /gene="rlmN"
                     /locus_tag="PLC06_08925"
     CDS             complement(751..1806)
                     /gene="rlmN"
                     /locus_tag="PLC06_08925"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRO90607.1"
                     /translation="MTSSNQSPINLYDLSRPALGDLLAGWGYSDYYASIVWSGLYRDL
                     ATNIAGIPALRPELAERLAAETGIGVLSMRSTIDSADGHTEKYLLGLADAQAIETVLM
                     YFRGRATACVSTQVGCAMGCIFCATGQMGFRRHLSQGEIMAQIVHVARTLRGRDDTLR
                     NIVLMGMGEPLHNYDATMAALDAAMDHRGLAIAPRFITLSTVGVVPGIRRLTEERRPF
                     RLAVSLHGATDEERTRLVPTARRWSLAELIAACRDYTQTLGRRIFFEWALIEGENDDP
                     EQAHALGHLLQGLDAHVNLIPLNPTIGFDGRPTQAPAVKAFQAVLAGYGLPSTVRQRR
                     GIDVAAGCGQLATLNAA"
     gene            1896..2672
                     /locus_tag="PLC06_08930"
     CDS             1896..2672
                     /locus_tag="PLC06_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRO90608.1"
                     /translation="MLNLYSWNVNGLRALHRKDVFLDWLATTQPDIVAVQETKCRPDQ
                     LDDILRDPPGYYSYWAAAERAGYSGTALFTRRKPLAVQIGLGLTDYDREGRTIVADYG
                     DFVLINAYFPNGSRDHSRVPFKMQFNADFLHYIEALRAEGRSVIFCGDVNTSHQEIDL
                     ARPRQNRNTTGFMDIEREWIDQVIAHGYIDTYRALNPDKTGAYTWWSQVSFSREKNVG
                     WRLDYFFVSPDLWPRVAGAGIHPDMPGSDHCPVSLTLDFT"
     gene            2674..4635
                     /locus_tag="PLC06_08935"
     CDS             2674..4635
                     /locus_tag="PLC06_08935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90609.1"
                     /translation="METDPAARSRFMGVARGEILVILLILIIAAVTRLGRPDLTEFKA
                     DEGRLLTLALEMAGGEFAWRGISSSTGFPNAPMSVWLYALPLRLWPHPYAATLFTGLL
                     GVAAVGGTYWLTRRYWGVRAAAVAALMLTVSPWAIIFSRKIWAQDLLPFFAVGWAIGA
                     TLAFVEGRKAFLLLHLVCLAIAVQIHPAAFGLVPATLLFLLVFRRRVDWRYVFLGGGL
                     AALTAAPFLWYLFGRRQAEGGLSLSSGGAVRPISLDSLRLIFTISTGEGARSLAGAGH
                     NGLPGETIVRWLWLAWIVCGVIWAIRQLIGRWDIPASKVAFICLVWFFVPALAFVWQW
                     TPVYLHYFIAVMPAPFVLAGAFFSRLLDALPPRGRVAAWAVVIALIAMQLASWVSVMA
                     AVAANPLAGGFGIPLGAKLSAADSARRLLNESGATEVLLAGDGSNPEREDFPAEFRAL
                     LHDVRLRYVDLNREAVFPGALSVVLLDTPLAGGPASTRELYERAAEDETLFPVSGTDL
                     AYTTMTLSPNSFPAAGVILQPEPLLANFVRFFGHNKLRLTPEGQLWDVYWRPAGNPDP
                     SDYHLFNHLIDGSGARIAQADGAAFSGDQWRAGDVVISRFMLPLTGEAQPPLTMRVGM
                     YRFPSLENVPVLDEAANPASDAIELPLNP"
     gene            complement(4625..5209)
                     /gene="folK"
                     /locus_tag="PLC06_08940"
     CDS             complement(4625..5209)
                     /gene="folK"
                     /locus_tag="PLC06_08940"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRO90610.1"
                     /translation="MPHNPTTNDQHRVAVILGSNIDREQNIPAAVRLLAEAATVIDVS
                     TVYETSAVGSCNQPGYFNAVVLLETALSPAELKDGLLADIEYRLGRRRTADKFAARTI
                     DLDIVTYDDESFDYTPADGRPRHLPDRDLLLYPHIAVPLAELLPDERHPETGETFSTI
                     AGRLLDEAAGHGYIIFPRPDVDLRSLLTGDGPTD"
     gene            complement(5274..5639)
                     /gene="folB"
                     /locus_tag="PLC06_08945"
     CDS             complement(5274..5639)
                     /gene="folB"
                     /locus_tag="PLC06_08945"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRO90611.1"
                     /translation="MDKVLIRDLLIRGIIGINPDERVKKQDILINIVIFTDIRRAAET
                     DDIDDTTNYKDISKRIIDFVEASSFLLVERLSTEIARLVLEDTTVSRVQVRVEKPGAL
                     RFARSVGIEIERSREDFGL"
     gene            complement(5664..6731)
                     /locus_tag="PLC06_08950"
     CDS             complement(5664..6731)
                     /locus_tag="PLC06_08950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRO90612.1"
                     /translation="MFILLMAVGALPAACGGAKKTELEPTAPAVAQATESGLALQATE
                     PSVPVLPPPAPTLSAPPSPSPAPTATTDPAAVATVAIMATQTAAADPPTPPVTAPATT
                     ESIVNMPMPTPSGIYSWTLKVPILMYHYVSEPPPDSDIYRVDLSVPPDAFRQQMAYLK
                     DNGYTPVDLYDLSTAIVAHTELPEKPIVITFDDGYLDNYENAFPILQEYGFKGTFFVI
                     TEFIDSGREGYMNWEMLEEMSRAGHRIESHSRTHPDLTTKSRDGLIWELLGSQETLAA
                     HIGYKPRYFCYPGGTYNDETIQVLKELDYWGAVTTENGSWHGFNDRFEWRRVRLRNST
                     TIQEFARLTDLEGTVSGKTPG"
     gene            6992..7405
                     /gene="erpA"
                     /locus_tag="PLC06_08955"
     CDS             6992..7405
                     /gene="erpA"
                     /locus_tag="PLC06_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HRO90613.1"
                     /translation="MDFEQLEQVEVETVYMTDTAAETVRNLLIQKNVPDYGLRVFVAG
                     GGCSGMQYGMALEAEPRNYDHIVEKDGVKVFIDPTSMMYLNQATIDYVDSIMGGGFKI
                     ENPNAVTSCGCGSSFKTQESEGYSEPAGGGCGCGH"
     gene            7495..>7696
                     /locus_tag="PLC06_08960"
     CDS             7495..>7696
                     /locus_tag="PLC06_08960"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HRO90614.1"
                     /translation="MSMRYFTLDEANTLLPELEPLVGRLLELRARVTHESRSMGNVLS
                     DLHSNVGGPSASRLTKEFEEIEL"
BASE COUNT         1381 a         2211 c         2399 g         1705 t
ORIGIN      
        1 aaaactgcgg cggtcgatgc acccgcctca acaaaacaag gagaaccaaa tgacccaggt
       61 agaaacaccg gccgttgctc ctcgagcagc ggccgttact tttagtcgag cggcgatcat
      121 cgctgtggcg gcctacgccg gagcgcaaat gctgtccgat atcgccagcc tgaagatcgg
      181 cgtcgtggcc ggattcgccg tcgatatggg cacctttatc tatcccatca cctttaccct
      241 gcgcgacgtg gcccacaaga tattgggtaa acgcaatgtc cgcaccttga tcgtcatggc
      301 tgcggtcatc aatctggtta tggcggccta cctggcgtgg gcggccaccg tgccgagcga
      361 tcccagctgg gggctgggcg attcattcag cgcgatcctg ggcccggtct ggcgtattgt
      421 gatcgcctcg attctggccg agctggtcag cgagctggtc gacaccgaga tctatcactg
      481 gtttgtgacg aaggtgacga cccgctacca gtgggcgcgc gtcctgtcca gcaacagcat
      541 cagcatcccc atcgataatt ttatcttcgc ggttggcgcg tttggcccat tgccggggct
      601 ggaaaatagc ccgctcagct tgccgtggcc ggtcgtgtgg cagatattcc tgttcaatct
      661 cgtggtgaaa tatttagtga cgctgatcag cttgccgctg atctacacga cgcccgacgc
      721 ggattggagc cggcgcgaag aaagcgatcg ctaggcggcg ttgagggtgg ccagttgtcc
      781 gcaaccggcg gcgacgtcga tgccccgccg ctggcggacg gtgctgggca gtccgtagcc
      841 ggccaggaca gcctggaacg ccttcaccgc cggcgcttga gtcggccggc cgtcgaagcc
      901 gatggttggg ttgaggggga tcagattgac gtgggcatcc agcccttgca gcaagtggcc
      961 caatgcgtgg gcttgttcgg ggtcgtcgtt ctcgccttca atgagcgccc actcgaaaaa
     1021 gatgcgccgg cccagggttt gggtgtagtc gcggcaggca gcgatcaact ccgccagcga
     1081 ccagcggcgc gccgtcggca ccagccgcgt cctttcctcg tccgtggcgc cgtggaggct
     1141 aaccgccaag cgaaacggcc gccgctcttc ggtcagccgc ctgatccccg gcacaacgcc
     1201 cacggtgctg agggtgatga agcgtggcgc gatggccaat ccgcggtggt ccatcgccgc
     1261 atccagagcg gccatcgtag cgtcgtagtt gtgcagcggc tcgcccatgc ccatcaggac
     1321 gatgttgcgc agggtgtcgt cgcgcccgcg cagtgtccgg gcgacgtgga cgatctgggc
     1381 catgatctcg ccctgggaca ggtgtcgccg aaagcccatc tggccggtgg cgcagaagat
     1441 gcagcccatg gcgcagccca cctgggtgct gacgcaagcc gtggcgcggc cgcggaaata
     1501 catgagcacc gtttcgatcg cctgagcatc agccagaccc agcaagtact tttcggtgtg
     1561 gccgtcggcg ctgtcgatgg tcgagcgcat ggacagaacg ccgatgccgg tctcggccgc
     1621 cagtcgttcc gccagttcag gccgcagagc aggtatcccg gcgatattcg tcgccaagtc
     1681 gcggtataga cctgaccaca ctatgctcgc gtagtagtcg ctgtagcccc agccggccag
     1741 aaggtcgccc agtgccgggc ggcttaaatc atagaggttg atcggcgact gattggaaga
     1801 agtcatcacg atggattgta gcccgatggg cggccggtaa caatccgtcg agcccgccgg
     1861 ctttccgcgc cgggcgttcg tgctaaaatc ggcccatgct gaacctctac agttggaacg
     1921 ttaatggtct gcgcgcgctg catcgcaagg acgtatttct cgattggctg gcaaccacgc
     1981 agcccgacat cgtcgccgtg caggagacga aatgccgtcc tgatcaactt gacgacatcc
     2041 tgcgcgaccc gcccggatac tattcctact gggcggcggc cgagcgcgcc ggttacagcg
     2101 gtacggcctt gttcaccaga cgtaaaccgc tcgccgtcca aatcggtctt ggcctgacgg
     2161 actacgaccg cgaagggcgc accatcgtag ccgattatgg cgacttcgtt ctcatcaacg
     2221 cctacttccc caacggcagc cgggaccaca gccgcgtgcc attcaaaatg cagttcaacg
     2281 cggactttct gcattacatt gaagcgctgc gggccgaggg gcgctcagtc attttttgcg
     2341 gcgacgtcaa tacatcccac caggagatcg accttgcccg gccccggcag aaccggaaca
     2401 cgaccggctt tatggacatc gagcgggaat ggatcgatca ggtgatcgcg catgggtata
     2461 tcgataccta ccgcgcactg aatccggaca agacaggagc ctacacctgg tggtcgcagg
     2521 tgagcttttc gcgggagaag aatgttggct ggcggctgga ctactttttc gtctctcccg
     2581 acctgtggcc ccgcgtggcc ggcgcgggga ttcatcccga catgccgggt tctgaccatt
     2641 gccccgtcag cttgacgtta gattttacct gatatggaaa ctgatccggc tgcgcggtcg
     2701 cggttcatgg gggtggccag gggagagata ctggtcatcc tcttaatcct gatcatcgcc
     2761 gccgtgacgc gcctcggccg ccctgacctg accgaattca aggcggatga gggtcgtttg
     2821 ctgaccctgg ctctggagat ggccggcggc gaattcgcct ggcgcgggat cagcagctcg
     2881 accggcttcc ccaacgcgcc catgagcgtg tggctctacg cgcttccgct gcggttgtgg
     2941 cctcatccct atgcggccac gttgttcact ggtttgcttg gcgtggcggc cgtcggtggg
     3001 acctactggc tcactcgacg ttattggggc gtgcgtgcgg cggccgtggc ggccctgatg
     3061 ctgactgtaa gtccgtgggc catcattttc tcgcgcaaaa tctgggcaca ggacctgctg
     3121 ccgttcttcg ccgtggggtg ggctatcggc gcgacgctgg cctttgtcga agggcgcaaa
     3181 gctttcctgc tcttgcatct ggtttgtctg gcgattgccg ttcagattca tcccgcggcg
     3241 tttggattgg tgccggcgac tctcctgttt ctgctcgtct ttcgccggcg agtcgattgg
     3301 cgctatgtgt ttcttggggg agggctggca gcgctgacgg ccgcgccgtt tctgtggtat
     3361 ttgttcggcc gccggcaagc ggagggcggc ctgtcgcttt cgtccggcgg tgcggttcgc
     3421 ccgatctccc tggattcgct gcggctgatc ttcaccatct ccaccggcga gggcgctcgt
     3481 tctctggcgg gggcaggtca taatggcttg ccgggggaaa caatcgtgcg ttggctatgg
     3541 ctggcgtgga tcgtctgtgg ggtgatatgg gcgatccggc aactgatcgg ccgttgggat
     3601 atacctgcgt cgaaggtcgc cttcatctgt ctcgtctggt tcttcgttcc cgcgctggcg
     3661 tttgtgtggc aatggacgcc ggtctatctc cattacttca tcgccgtaat gcccgcgccg
     3721 tttgtgttgg cgggtgcgtt cttcagccgc ctgcttgatg cacttcctcc ccgtggccgg
     3781 gtggctgcct gggccgtggt gattgccctc atcgcgatgc aactggcgtc ctgggtgagt
     3841 gtgatggccg ctgtggccgc caacccgttg gccggtgggt tcggaatccc actgggggcc
     3901 aagctgtcgg ccgcggatag cgcgcggcgt ttgctgaatg agagcggggc gacggaggtg
     3961 ttgctggcgg gcgatggctc gaaccctgag agagaagatt tccccgccga gttccgggcc
     4021 ttgttgcacg atgtgcggct gcgctatgtt gatctcaacc gggaagccgt gttccccggc
     4081 gctctgagcg tcgtgctgct ggatacgccc ctggccggcg gcccggcttc gacgcgtgag
     4141 ctttatgagc gggccgccga agatgaaacg ttattccctg tctccggtac agatttagcc
     4201 tatacgacga tgacgctatc gccaaactct ttccccgcgg ccggcgtcat cttgcagccg
     4261 gagcctcttc tggcgaattt tgtccggttt tttggccaca ataagcttcg cctgacgccg
     4321 gaaggtcaat tatgggatgt ttattggcgc ccggccggca accctgatcc gtcggattat
     4381 catctgttta accacctgat tgacggcagc ggcgcgcgca tagcccaggc ggatggagct
     4441 gcgttttccg gtgaccaatg gcgcgcgggc gatgtggtca tcagtcgctt tatgttaccg
     4501 ttgacgggcg aggcgcaacc gccgctaacc atgcgtgtgg ggatgtatcg ctttccttct
     4561 ctggagaatg tgcccgtgct ggatgaagcc gccaatccgg catcggatgc catcgaactc
     4621 cctctcaatc cgtagggcca tcacctgtta aaagagatcg caaatccaca tccggccgcg
     4681 ggaaaatgat gtacccatgc ccggccgcct catccagcag tctgcccgcg attgtgctga
     4741 aggtctcgcc tgtctcggga tggcgttcgt ccggcaacag ttccgcgagc ggcacggcaa
     4801 tgtggggata gagcagcaag tctctatcgg gcagatggcg agggcggcca tctgcgggtg
     4861 tgtaatcgaa tgattcgtcg tcataggtga cgatatccag gtcgattgtg cgcgccgcga
     4921 atttgtcggc cgttcgtcgc cgccccaaac gatattcgat atcggcaagc aggccatctt
     4981 tcaactctgc cggtgatagt gcagtttcca gcagcaccac agcgttaaag taaccgggct
     5041 gattgcatga cccgacggcc gacgtctcat aaacggtcga tacgtcgatt accgttgccg
     5101 cctcggccag cagccggacg gcggccggga tgttctgctc cctgtcgata ttcgagccga
     5161 gaatgacggc gacgcgatgt tgatcgttgg ttgtgggatt gtgcggcatt attgttcctg
     5221 ggggaatccc acgcttattg gtgacgcaaa accggacggg ttcgctctct tatctagagg
     5281 ccaaaatctt cgcgagaccg ctcgatctcg atgcctaccg agcgggcaaa acgcaacgcg
     5341 cccggctttt ctacccgcac ctgaactcga ctgacggttg tgtcctccag aaccagccgc
     5401 gcgatctccg tcgatagccg ttccacgaga aggaaggatg acgcttcgac aaaatcgatg
     5461 atgcgtttgc tgatgtcctt gtaattggtg gtgtcgtcga tatcgtcggt ctcggccgcg
     5521 cgtctgatgt cggtgaagat gacaatgtta atgagaatat cctgcttttt gactcgttca
     5581 tccgggttaa tgccgatgat gccacgaatc agcagatcgc gaatcaatac cttatccatc
     5641 tatatcctgt ttccttggct gaatcagccg ggtgtcttgc cgctaacggt gccttccaga
     5701 tcggttagcc gcgcaaattc ctgtatggtc gttgagttgc gcaagcgcac acgccgccac
     5761 tcgaagcggt cgttgaatcc gtgccagctg ccgttctcgg ttgtgactgc tccccaataa
     5821 tccagctcct tcaagacctg aatcgtctcg tcattatacg tcccgcccgg ataacagaag
     5881 tatctgggtt tatagccgat atgggccgcc agggtttcct gcgagccgag gagttcccaa
     5941 atgaggccgt cgcgcgattt ggtggtcaga tcggggtgag tgcgcgagtg ggattcgata
     6001 cgatggccgg cgcgcgacat ctcttcaagc atttcccaat tcatatagcc ttcgcggccc
     6061 gaatcgatga attccgtgat cacgaagaag gtgcctttga atccatattc ctgaaggatg
     6121 gggaaagcat tctcatagtt atcgaggtaa ccgtcgtcaa acgtgatcac gattggcttt
     6181 tcgggcagct cggtgtgggc cacgatagcg gttgacagat cgtacaggtc aactggtgta
     6241 tatccgttgt ccttcaggta agccatctgt tgccggaagg cgtcgggcgg cacggagaga
     6301 tccacccggt agatgtcgga atcgggcggc ggctcactga cataatggta catcaggatc
     6361 ggcaccttca gcgtccagct gtagatgccc gatggcgtcg gcatgggcat gttgacgatg
     6421 gattccgtcg tggccggcgc cgtcaccggc ggggttggcg gatcggcggc ggccgtctgt
     6481 gtagccatga tcgccacggt cgccacggct gccggatcgg tagtcgcagt tggcgccggg
     6541 gagggcgagg gtggggccga tagcgttggc gcgggcggtg gcaataccgg cacggaaggc
     6601 tctgttgcct gtaatgctaa ccctgattcg gtcgcctgtg cgacggccgg ggcggttggc
     6661 tcgagttcag tcttcttcgc accaccacaa gcggccggca gcgcgccgac cgccatcaat
     6721 aaaataaaca tcaaaatacc caacctggcg ggcgggtcat gtcgtctttc gttcattaag
     6781 agcttccaca aatcatgcat cttgtctgtc agaatagcca accggtaata ctccgtcaag
     6841 cgcgcaatac cctttcgtag agaatgctga gcaatcgctt gaacgatcgc gaccaggtat
     6901 cctattatga tgaatttatt gacgaaaatt gactttcaac aggcccactg atagaatcac
     6961 atctatattt tcaggttgag gactgcctat catggatttt gagcaattgg aacaggtcga
     7021 agttgagact gtctatatga cggatacggc ggcggaaacc gtgcgcaacc ttctgatcca
     7081 gaagaatgtt cccgactatg gcttgcgcgt atttgtggcc ggcggcggct gctctggcat
     7141 gcagtatggc atggcccttg aggccgagcc gcgcaactat gatcacatcg tcgagaagga
     7201 cggcgttaag gtcttcattg atccgaccag catgatgtac ctgaatcagg cgacgatcga
     7261 ctatgtcgat agcatcatgg gcggcggctt caagatcgag aacccgaacg cggtcacttc
     7321 ctgtggttgt ggctcttcct ttaagacgca ggagagcgaa ggctactccg agccggccgg
     7381 tggtggctgc ggctgcgggc actgatttcg ttcatcgcac cggatatata acgagggtgg
     7441 tgggtctgtt ggccgccacc ctttttcttt atcggcgggc cgaggaattg atcgatgagc
     7501 atgcgctatt tcacgctgga tgaggcgaat accctgttgc cggagctgga gccattggtc
     7561 ggccgcctgc tcgaattgcg cgcccgtgtg actcatgaga gccggtcgat ggggaacgtc
     7621 ctgtctgatt tgcatagcaa tgtgggcggg ccgagcgcct ctcggctgac gaaagagttt
     7681 gaggagatcg agctac
//
LOCUS       DAOMQG010000138         7675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4102_length_7675_cov_9.274409, whole genome shotgun sequence.
ACCESSION   DAOMQG010000138 DAOMQG010000000
VERSION     DAOMQG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_7675_cov_9.274409"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLC06_08965"
     CDS             complement(<1..730)
                     /locus_tag="PLC06_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO90615.1"
                     /translation="MYERVLVIDDSQEIRDFLVENILRPKGFEVLAASDGIMGLELAI
                     SKEPDLMITDQQMPGLTGLQVLQKLRERGVDIPAILITGEGSEDTAVRAFRLGIKDYI
                     IKPIDAEELSQSVDKALRENRLQKERDQLVTQLMESNSQLQRRAQELNVLYGVGKSVS
                     SSLDLEEVLQRVVEAAVYVVGAEEGSLMLLDEDRGELYIRASKNLDSKARSMRKRVTD
                     SLAGKVLQTKRAIAIGADSQWKRTH"
     gene            1023..2756
                     /locus_tag="PLC06_08970"
     CDS             1023..2756
                     /locus_tag="PLC06_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO90616.1"
                     /translation="MTRIVGQIAFATSTPDPLATPVRVEPLAILDISLIGQLPDLDPG
                     LAERQSQLDLTQNLFTGLTNFNPETNSIEPELASTWAVSDDGRTWTFNLRDDIYWVRP
                     KSPGPGSQALWSATQIRPVTAEDVVYAVQRYCSRQVTSAATFSLFLIEGCEAVYAIPE
                     PVEADLKTIGIEAVNPTLLQVRLTKPAGFFLTLTSMPFFQPVPKDLVEEMGNEWLNPA
                     GEFSIGWQTPRNLITSGPFLPVSTQLTFQTATLHRNPLWPKDLDKPGNIEVINISFLD
                     EDTEAFAMWQDRLLDIAPLPTAERESFLQRTEAKVRTIPDQVLFYLGFNFNSGVFHEV
                     EVRRAFSAAINRQELVTELYGGRGIVMRHATVPGIVAAIPADELGVGYSPDFARQQMA
                     ASSFRNCRLMPEITFLVSSADLSLRQAELVRDMWIKELDCLKESIHIKQVSFGELLVG
                     TQPDATARPDMWEFAWAPTFPDAKNSITDLLHCRDSENRQNRECSDADSLLSRAGTTV
                     DTTERTGLYRQAESIFFNETGSFPIIPLYIRAREIVVHDWIQFTPVAFGGQQWDRILL
                     DVTLKELERSR"
     gene            2757..3464
                     /gene="rsmG"
                     /locus_tag="PLC06_08975"
     CDS             2757..3464
                     /gene="rsmG"
                     /locus_tag="PLC06_08975"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRO90617.1"
                     /translation="MDELSAWATTFGVTLTPRQSEQFALYETLLLEWNERISLTAIRD
                     PREMRIRHFLDSLSCVTATGPLDECSLIDIGTGAGFPGLPLKIAFPGLRLTLIDSVTK
                     KARFLELVVNELGLGDVSVIADRAENLGQDARFRESFDWATARAVADLRVLVELLLPL
                     VRVGGHALAQKGESAPGELAAAETAISILGGGAPSLSAVHLPETDKTHYLVTIAKQRS
                     TPSGYPRRTGVPAKRPL"
     gene            complement(3461..4747)
                     /locus_tag="PLC06_08980"
     CDS             complement(3461..4747)
                     /locus_tag="PLC06_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRO90618.1"
                     /translation="MPIGLFGFIFPSMQVRRLSLTNFRNYSRLELDLPGGIILLHGDN
                     AQGKTNLLEAIYFLATTRSPHASQDQQLINWDALETDEPVVVGRLVADVERPDAPAHL
                     EMRLIMEEHGSLMGNGASGFRREALINRRKVRLMDLLGQLRVVLFLPEDVELATGAPA
                     NRRRYMNVTLCQVDSNYCRDLSAYNKVLEQRNALLRRMAEGQMRHAADVLQVLTEKLI
                     EPGSRVLRRRGRFIRDMAQQAQEIYFQDLIGGKESLRLGYMPGWYANGRKTADEQLAD
                     GEWLQANEDLGAVQARFAAELEVARAADLARGSSTVGPHRDDWAILVNGKNLGQFGSR
                     GQVRTAILALKLAEINWMKAETADVPILLLDEVIAELDLHRRAALLAYVADQVRGKGM
                     AQALLTATDPGMFPEEFLGQVARLLVRDGRASLEQV"
     gene            4887..6491
                     /locus_tag="PLC06_08985"
     CDS             4887..6491
                     /locus_tag="PLC06_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRO90619.1"
                     /translation="MNSPDIIFIVLDTQRADRLGAYGHHRPTSPNIDRFAAEGALFEQ
                     AVSPAQWTIPSHASLFTGLYPTSHGVTQSSQSLSPGRPHLAEVIRRLGYETIGFCNNP
                     LVGVLNNGFKRGFQTFYNYGGAFPSVPDSSNPWPWPVSLIAEAYTQFLRRISYPIQNY
                     IGRSDLAFSISLHAWLTPLWSKIANFKGQNERSVQDVVHFLDRREEQKPDEPLFLFMN
                     LMETHLPFWAPGEFVDRIAPYFRHSKEARTVMRAWNREAYRWAAPLAEPLSEMEEKVL
                     NDMYDAEVAYQDHYLQNLFDALARRERSNDTLTIIVADHGDGLGDHDFFGHAFVAYEE
                     LVHVPLIVHWPGRIEPNTRVDTPVSTRRVFHTIVDAAAAGRNEALDAALEDDAPNVRR
                     LSLRHTLAGRDPEQQTAFSEIIPPMNFVKAIEARQPELLSRFRCLSTRRAVVRSSRED
                     DGVPFKMIETDDIAEELYNLTADPLELDNRFAGEPSLVAGLELTIEQMVQRATRDRSA
                     QPAGATIDIDGDELLRQRLRGLGYLE"
     gene            6562..7449
                     /gene="lgt"
                     /locus_tag="PLC06_08990"
     CDS             6562..7449
                     /gene="lgt"
                     /locus_tag="PLC06_08990"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRO90620.1"
                     /translation="MLDGINIDPIAFTIPIGGGFPIYWYGIIVTVGIAVGVFWAAREI
                     ARRNQNVDEFFNALILVIFSGYVFARLTYVLLEMFGGRGDQFKSFWDVINIRSGGINI
                     LGGFIGAAIVGWLYIRWRRLNFWQYADVAGPALLVAQGIGRWGNFINQELYGPPTTLP
                     WGILIDAPYRLAKYADMTQFPPETRFHPTFLYESIWLILGFVVLIVLNNKFRDKWKIG
                     TLFGLFLMWWGAGRAFLEFFRPDQATIGNSPITYSFLLALGLALTGLYIVLSRNNRMP
                     QIFSRRRQRVVKPKPRRES"
     gene            7488..>7675
                     /locus_tag="PLC06_08995"
     CDS             7488..>7675
                     /locus_tag="PLC06_08995"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRO90621.1"
                     /translation="MRFASEFGELALTFDDVLLVPGYSQVLPAQVDLRTRLIDGIHLN
                     VPVISAAMDTVTEAQMAIG"
BASE COUNT         1545 a         2402 c         2107 g         1621 t
ORIGIN      
        1 tatgggtgcg tttccattgc gagtctgcgc caatagcgat ggcgcgcttg gtctgcaaca
       61 ccttgccggc caggctgtcc gtcacgcgct tgcgcattga acgcgccttg gaatccaggt
      121 tctttgaagc gcggatgtat aactcgcccc gatcctcatc aaggagcatc aaggagcctt
      181 cttccgcgcc gacgacatag actgccgcct caaccacgcg ctgcaacacc tcttccaaat
      241 caagtgatga ggacaccgac ttgccgacgc catagagcac attcagttcc tgcgcgcgcc
      301 gctggagctg ggaattgctt tccatcaact gagtgaccag ctgatcgcgc tccttttgca
      361 ggcgattctc gcgcagggct ttatccaccg attgactaag ctcttctgca tcaatcggct
      421 tgatgatgta atccttgatg cccaggcgga aggcgcggac ggcggtatcc tctgaccctt
      481 cgccggtgat caggatagcc gggatgtcaa cgccacgttc gcggagcttt tgcaggacct
      541 gcagacctgt gagacccggc atctgctgat cggttatcat gagatcaggt tctttggaga
      601 tggccagttc cagacccatt atgccgtccg aggcggcgag cacctcaaac cctttgggcc
      661 gaagaatatt ctcgacgagg aagtcccgaa tttcctggga gtcgtcaata accaatactc
      721 gttcgtacat aacgtttgct gcttcctgac gcaacacaga cggacaacgg aatatatatt
      781 actactcgga taatatcgag gcaactgcag ccgtttccct tgtcgagcag ccgggagtga
      841 tatagcatgc gctcccaatg ttcattgtgg gtaacaagaa acacgcgcaa atcactcggc
      901 aatggcgtct gtggctcctg gccggcttat tgatttttgc cgccggatgc cgcgccaata
      961 ccgcgtcttc ctcaatcggc gagggaaccc cgcagccgac tgccggcaga gtccaggcca
     1021 ttatgacccg gatcgtcggt cagatcgctt ttgccacaag cacgcccgat ccattggcaa
     1081 cccccgtgcg cgtcgagccg ctcgccatac tcgacatcag ccttattgga cagctgcctg
     1141 atctcgatcc cggtctggcc gaacgtcaat cccaactcga cttgacgcaa aacctgttca
     1201 ccggattgac gaacttcaat cctgagacaa acagtatcga gcctgaattg gcatcgacgt
     1261 gggcagtcag cgacgacggc cgcacgtgga cattcaatct acgtgatgac atttactggg
     1321 ttcgccccaa aagccccggg cctggcagcc aggctctttg gtctgcgact cagattcggc
     1381 cggtcaccgc agaggatgtt gtctatgccg ttcaacgcta ttgcagtcgt caggtcacaa
     1441 gcgcggcgac attttccctg tttctcatcg agggttgtga ggccgtctac gcaatcccgg
     1501 agcctgttga agccgacctg aaaaccatcg gcattgaggc agtcaatccc accttgctgc
     1561 aagtcagatt aacgaaaccg gccggcttct tcctgacgct caccagcatg cccttctttc
     1621 agccggtacc caaagatctc gtcgaagaaa tgggcaacga atggttgaac ccggccggag
     1681 aattcagcat cggttggcaa acgcccagga atctgatcac ctcaggtcca tttctgccgg
     1741 tatcaacaca attgacattc caaaccgcta cgcttcatcg caacccgctg tggcccaagg
     1801 acctcgacaa accgggaaat atcgaagtca tcaacatcag cttcctggat gaagacactg
     1861 aagccttcgc gatgtggcag gatcgcttgc tcgatatcgc gccactgccc accgccgagc
     1921 gagaatcatt tttgcaacgt accgaggcca aggtgcgtac cattcccgat caggtcctgt
     1981 tctatcttgg gtttaacttc aacagcggcg tctttcacga ggtcgaagta cgccgcgcct
     2041 tcagcgcggc gatcaaccgg caggaacttg tcaccgagct atatggcggt cgcggcatcg
     2101 ttatgcgtca cgctaccgtc ccgggcatcg tcgccgccat ccccgcggac gaactcggcg
     2161 tcggttacag ccccgacttc gcacgacagc aaatggctgc cagctcattt cgcaattgcc
     2221 ggctcatgcc cgagataacg tttctggtga gctcggccga cctgtccttg cgccaggcag
     2281 agcttgttcg cgacatgtgg attaaggagt tggactgcct gaaagagagc atccacatca
     2341 agcaagtatc gttcggtgaa ctattggtcg gtacacagcc ggacgccacc gcccgaccgg
     2401 atatgtggga gtttgcctgg gcccctactt tccccgacgc caaaaattcg attaccgatc
     2461 tgcttcattg tcgcgacagc gaaaaccgcc agaatcggga atgttccgac gccgattctt
     2521 tgctcagtcg cgccggcacg accgtagaca caaccgagcg cacaggactt tatcgccagg
     2581 cggaatctat cttcttcaac gagacgggca gctttcctat catcccactg tatatccggg
     2641 cgcgcgagat cgtcgttcac gactggatac agttcacgcc ggtggccttt ggcggccagc
     2701 agtgggatcg tattctgctc gatgtcacct tgaaggaact ggagcgaagc cgctagatgg
     2761 acgaattaag cgcctgggcc acaacctttg gagtcacatt gactccccgt caatctgagc
     2821 agttcgcgct ctatgagact ctgctcctgg aatggaacga gcgcatctcc ttgaccgcta
     2881 tccgcgaccc gcgcgagatg cgaattcgcc acttcctcga ttcgctctcc tgcgtcaccg
     2941 cgaccggacc gttggacgag tgttcgctga tcgatatcgg caccggcgcg ggctttcccg
     3001 ggttgccgct caagatcgcc ttccccggcc tgcgcctgac gctgatcgac agcgtgacta
     3061 aaaaggctcg ctttttggag ctggtcgtca atgaactggg cctgggcgac gtatccgtga
     3121 tcgccgatcg tgccgagaac ctgggccagg atgcgcgctt ccgggaatca ttcgattggg
     3181 ccacggcgcg cgcggtggcc gatctgcgag tattggtcga gttactgctg cctctggtgc
     3241 gtgtgggcgg ccatgcactg gcccagaaag gggagagcgc gccgggcgaa ctggccgcgg
     3301 ccgaaactgc tatctccatc ctgggcggag gcgcgccaag cctgtcggcc gtgcacctgc
     3361 ccgaaacgga caagacccac taccttgtca ccatagccaa gcagcggtcg acaccctccg
     3421 gctatcctcg tcgaaccggt gtccctgcca agcgtcctct ctaaacctgt tcaagcgaag
     3481 cacgtccatc gcgcaccaga agcctcgcca cctgccccag aaactcctca gggaacatgc
     3541 ccggatccgt cgccgtcaat aacgcctgag ccatcccttt cccgcgcacc tggtcagcta
     3601 cataggccag gagcgctgcc cgacggtgca ggtcgagttc agcgatcacc tcgtcgagta
     3661 acagaatggg cacatcggcc gtttccgcct tcatccagtt gatctcggcc agcttgaggg
     3721 ccagaatagc cgtgcgcacc tgaccccgcg agccaaactg ccccaggttc ttgccattca
     3781 ccagaatcgc ccaatcatcc cggtgtggcc cgaccgtcga agagcctcgc gccagatcgg
     3841 cggcgcgggc gacctccagt tcggccgcga atcgcgcctg gactgcgccg aggtcttcat
     3901 tcgcctgaag ccattcgccg tcggcaagtt gctcgtcggc cgtcttccgc ccattggcat
     3961 accagccggg catatacccc agccgcaggg attccttgcc gccgatcagg tcctggaaat
     4021 agatctcctg cgcctgctgg gccatatcgc gaatgaaacg ccctcgccgg cgcaaaaccc
     4081 ggctgccggg ttcgatcaat ttctcggtca ggacttgcag cacatcggcc gcatggcgca
     4141 tttgcccttc agccatccgg cgcagaagcg cgttgcgttg ctccagcacc ttgttgtagg
     4201 ccgacaaatc gcggcaatag ttggaatcaa cctgacagag cgtgacgttc atgtagcgtc
     4261 gccggttggc cggcgcgccg gtcgccaatt ccacatcctc aggcaaaaac aaaaccacgc
     4321 gcaattgacc taacagatcc atcaaccgca ccttgcggcg gttgatcaac gcctcgcgcc
     4381 gaaaaccact cgccccgttg cccatcaggc tgccgtgctc ctccatgatc aggcgcatct
     4441 ccagatgagc cggcgcgtcg ggtctctcca cgtcggcgac caggcggccg acaacgaccg
     4501 gctcgtccgt ctccagagca tcccaattga ttagctgctg atcctggctg gcgtggggcg
     4561 aacgcgtcgt tgccagaaaa taaatcgctt ccagcaaatt cgtcttgccc tgggcattgt
     4621 cgccatgcag cagaatgatc ccgcccggca gatccagctc taggcgagaa tagttgcgaa
     4681 agttcgtcag cgaaagtcgg cgcacttgca tggaaggaaa gataaagcca aacaggccga
     4741 ttggcaaacg tcgctatcct catccaaata aagagaggct tgagagggtt tgcgacaccc
     4801 tatctcacat gctatgcttt ccaatcatat ttgcgtcaga attgcggcgc actcatcgtt
     4861 gagagagcag cgtatcaggt agatcaatga attcgccgga cattattttt atcgttttgg
     4921 acacccaacg ggccgatcgc ctcggtgcct atgggcatca tcggccgacc agccccaata
     4981 tcgatcgctt cgctgccgag ggggcgctct ttgaacaggc cgtttcgccc gcccaatgga
     5041 ccatccccag ccacgcctca ctcttcaccg gtctgtatcc cacatcccac ggcgtcactc
     5101 aatcttccca gagcctcagc ccagggcgtc ctcatttagc cgaggtaatt cgccgtctcg
     5161 gctacgagac gattggtttt tgcaataatc cgctcgtcgg cgtcctgaat aacggcttca
     5221 agcgtggctt ccagacgttc tataactatg ggggcgcgtt tcccagtgtc ccggattcat
     5281 cgaacccctg gccctggccg gtcagtctga tcgccgaagc gtacacccag ttcctgcgtc
     5341 ggatctccta tcctatccag aactatatcg gccgttccga tttggcgttt agcatatcgc
     5401 tccatgcctg gctgacccct ttgtggtcga agatagccaa tttcaagggt cagaatgagc
     5461 ggtctgtgca ggacgtcgtc cattttctgg atcggcgcga ggaacagaaa cccgacgagc
     5521 cgctgttcct gttcatgaac ctgatggaga cgcatctccc gttctgggcg ccgggtgagt
     5581 tcgtcgatcg catcgcgccc tatttccggc acagcaagga agcccgtacg gtcatgcgcg
     5641 cctggaatcg ggaagcctat cgctgggcag ctcccctcgc cgaaccactc agcgagatgg
     5701 aagaaaaagt gctcaacgat atgtacgacg ctgaggtcgc ctatcaggac cattacctgc
     5761 aaaacctgtt cgatgcgctg gcccggcgcg agcgctccaa cgatacattg accatcatcg
     5821 tggccgatca cggcgacggc ctgggcgatc atgatttctt cggtcacgcc ttcgtggctt
     5881 atgaagagct ggtccatgtg ccgctcatcg ttcattggcc cggccggata gagccaaaca
     5941 ctcgcgtcga tacgccggtc agcacgcgcc gcgtgttcca cacgatcgtc gatgccgcgg
     6001 ccgccggccg gaacgaagcg ctcgatgccg cgctcgagga cgatgcgccg aacgtccgcc
     6061 ggctcagtct gcggcacact ctggccggcc gggaccccga acaacaaacg gctttcagtg
     6121 agatcatccc gcctatgaac ttcgtgaaag cgattgaggc gcggcagccg gaacttctct
     6181 cccggttccg ctgcctgtcc acacgtcgcg cagtcgtgcg ctcctcgcgt gaggacgatg
     6241 gcgtgccgtt caaaatgatc gaaaccgacg atatagccga ggagctatat aacctgaccg
     6301 ccgatccgtt ggaactggat aatcggtttg ccggcgagcc gtcgctggtg gcgggcctcg
     6361 aactgaccat cgagcaaatg gttcagcgtg ccacacggga ccgcagcgcg cagcctgccg
     6421 gcgcgacaat cgacatcgac ggcgacgagc tgctccgcca gcggttgcgc ggcttgggct
     6481 atctggaata attaggcgga atccggccgg tccccgcacc ggatccggtc gaaacattat
     6541 ctttattgta acgggtgaac tatgctggac ggaatcaaca tcgatccgat cgccttcaca
     6601 atccccatcg gcggcggatt tcccatctat tggtacggta tcattgtgac ggtgggcatc
     6661 gccgtcggcg ttttttgggc ggcgcgagaa attgcccggc gcaaccagaa tgtcgatgag
     6721 tttttcaatg ccctgatcct ggtcattttc agcggctacg tcttcgcccg cctgacctac
     6781 gtcttgctgg agatgtttgg cggccgtggc gaccagttca aaagcttttg ggacgtgatc
     6841 aacatccgct ccgggggcat caacatcctg ggcgggttca tcggcgcggc catcgtcggc
     6901 tggctctaca ttcgttggcg gcgactgaac ttctggcaat atgccgacgt cgccggaccg
     6961 gcattgctcg tggctcaggg cattggccgt tggggcaact tcatcaacca ggaactttat
     7021 ggtccgccga cgacgttgcc gtggggtatc ctcatcgatg ccccctatcg tttggccaaa
     7081 tacgccgata tgacccagtt cccgccggag acgcgcttcc acccgacgtt tctctatgag
     7141 tcgatctggc tcatactggg cttcgtggta ttgatcgtcc tcaacaacaa gttccgcgac
     7201 aagtggaaga tcggaactct gttcggcctg ttcctcatgt ggtggggcgc gggtcgggcg
     7261 ttcctggagt ttttccggcc cgaccaggcg acgatcggta attcgccgat cacctattca
     7321 ttcctgttgg ctctgggcct ggcattgaca ggcctgtaca tcgttctgtc gcgcaataat
     7381 cgtatgccgc agatcttcag ccgtcgccgc cagcgcgtcg ttaaacccaa accgcgccgc
     7441 gaaagctaag ggataaccgg cagtcaccat gccggaagag ggtgagtatg agattcgcat
     7501 ctgaattcgg cgaactcgcg ctgacgttcg acgatgtgct gctcgtgccc ggctactcgc
     7561 aggtgcttcc ggcccaggtc gacctgcgca cgcggctgat cgacggcatc catctgaatg
     7621 tgccggtcat ttcggcggcg atggacaccg tcaccgaggc ccagatggcc atcgg
//
LOCUS       DAOMQG010000139         7667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4108_length_7667_cov_11.469785, whole genome shotgun sequence.
ACCESSION   DAOMQG010000139 DAOMQG010000000
VERSION     DAOMQG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4108_length_7667_cov_11.469785"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLC06_09000"
     CDS             complement(<1..410)
                     /locus_tag="PLC06_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO90622.1"
                     /translation="MIVVDVNVIAYLFITGEKTTEARTVRELDADWIVPDLWRDEFLN
                     ILATYVRHGGIDLETAVALWLSAVTLFKGHESTADPIMALELAARHQLSAYDAQYLVV
                     AIGNEARLVTEDKAMLAAVPQRCMTMRDFISANTP"
     gene            complement(407..625)
                     /locus_tag="PLC06_09005"
     CDS             complement(407..625)
                     /locus_tag="PLC06_09005"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRO90623.1"
                     /translation="MTDLLIRQMPDELHARLKQSARANRRSMNQEIIILLERSLAEEE
                     PQPLPQPIRGAFLIDDEWLQKAREEGRS"
     gene            complement(686..1222)
                     /locus_tag="PLC06_09010"
     CDS             complement(686..1222)
                     /locus_tag="PLC06_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90624.1"
                     /translation="MHETLTAVNESTVTGLGRLARFFGFSDVMGRLYGTLLLSPGPLS
                     LDDLGDTLEISKGSVSMNMRGLERWGMAKEVWVRGERRKFYKAESDMWQVIRNVLESR
                     EQREVHLALHVLGESIEKLQAAECDLSAEEQQLARYYLDRIGDLQAFFQFAQLALHAV
                     LGSEKALDFEAITKIEIE"
     gene            complement(1397..2293)
                     /locus_tag="PLC06_09015"
     CDS             complement(1397..2293)
                     /locus_tag="PLC06_09015"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRO90625.1"
                     /translation="MTLNSSLPLLTIAGSDSGGAAGLQADLKTWAALSENGVRVYGMS
                     AITAVTAQNSERVAAVHYLPPAFVAAQIDAVLSDYGAAGIKTGFLGRADTIKIVAERL
                     NHWRERLHPRPAIIIDPVLVNHRGEAMFGPEVAAAYREWLFPLADLVTPNAAEAALLG
                     WSLESTFAGGPPADDQRPPTEVPGSDHPASDREQPSVAQWLIKSLRRGDAISDAWWDG
                     QSWTELPQPRIDTINTHGSGDTLSAAVCAYRAMGHTPLDAIRRAQAFTHAAIRRGAPW
                     RLGAGHGPLGHSVVDRRQTTDH"
     gene            2469..3275
                     /locus_tag="PLC06_09020"
     CDS             2469..3275
                     /locus_tag="PLC06_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO90626.1"
                     /translation="MMTTPEKQAQPSPDMLSPSGSYAGEIRRIGQLVGRGQWRTALHR
                     ADSLGANFWNFEVRGGSRRVQCPCCGWEGPAFVSLSNWRAVQHNSRCPNCDSRSRHRG
                     LTILLPRLLAAMPDGQALVFAPEAALLALVRQHARAGVETTDYLMEDVDHPGEDIQRL
                     TFPDAHYGALMCNHVLEHIPDDRAALAECARVLMPGGVAVFTIPGDYDQTATRYFDLP
                     DPNGHLRHYGLDVLEKMRAVFSRVEAVDMSLGTNPRQHIHPGDMAFVCYK"
     gene            complement(3331..3897)
                     /locus_tag="PLC06_09025"
     CDS             complement(3331..3897)
                     /locus_tag="PLC06_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90627.1"
                     /translation="MNDDPPADEAQRNVWLHYLLDLEISPALPPSHRLLLGILRQAVV
                     DYFGDDPVHQFSAALYFANSPLYRETLQAFNLPDDALPLGVDLTAFRRKEKMDPQDEP
                     DSLQLETLVRELTGNQLKIVLTMGLMELPVVTRKISLRCDLTRSTVLIAMNHLESQGL
                     VVRHEFDGRPAWSFPAAVGKIINEVWRS"
     gene            4008..4607
                     /locus_tag="PLC06_09030"
     CDS             4008..4607
                     /locus_tag="PLC06_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90628.1"
                     /translation="MVSYRIRRLPADDEPGRRYHLFDRADQLLLVADQGSAWLPAGNV
                     AQVRFARPGGEQVASMDLPGPAGGRPERKQNYAIIYEHAVYALLTAKDLSSGTGTLTQ
                     ALVPAKFDRLIIEVEGYKWLGLCWTGGDNGRLLVLYDMETTGLSSSVDPESADLPEPI
                     GLLEVGAGDYDFDLTLPAGRLTQGPLLGLALAYLVDGFL"
     gene            complement(4619..5047)
                     /locus_tag="PLC06_09035"
     CDS             complement(4619..5047)
                     /locus_tag="PLC06_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRO90629.1"
                     /translation="MVSPLLPHRIPRAVRAGEKVAFSRTLTEADAAIFIGITWDVNPY
                     HTDDAYVAQTRFGRRIVPGLLTAGLLTHLGGLWAFLATDMSFEFIGPVYIGDTVTAES
                     EVVEVSDKGWVRLAGRVIGPDGTDVLRATIRGYPGRFEDR"
     gene            complement(5054..6418)
                     /locus_tag="PLC06_09040"
     CDS             complement(5054..6418)
                     /locus_tag="PLC06_09040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRO90630.1"
                     /translation="MNKRKEHIVIVGGGFGGLYAIKAMKHAPVDVTLIDRRNFHLFQP
                     LLYQVATGGLSPEDIASPLRAALKDQKNAQFLTGQVIDIDPERKVVILEDGDEIAYDA
                     LIVATGASHHYFGHDGEWERLAPGLKTIEDAVKIRRRILLAFEAAERETDPERRRALM
                     TFVIVGGGPTGVELAGALGELATHTMREEFRAIDTADAQIDLIEGQDRLLSAYPEELS
                     ATAADALRGLGVTVQTNKLVTEIADDYVLVKDTKSGEQTLIPTFTILWGAGVKASPLS
                     DVLAHRAGAELDRVGRVIVRPDLTIAGHPDIFVIGDLAHYDHQTGKPLPGVAQVAMQM
                     GKYVADVLRRRRFDKEIRPFVYKDKGMLAVIGRNKAVADIGNLHFAGFPAWLIWVFVH
                     INFLIEFDNKLKVMTQWGWNYLTRRQGARLITGEAAPVWMQLPIGHEKTAHVGKNVAH
                     KIDG"
     gene            6834..7256
                     /locus_tag="PLC06_09045"
     CDS             6834..7256
                     /locus_tag="PLC06_09045"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRO90631.1"
                     /translation="MAIRHADAQWEGTLKEGSGYLKLESGVYAGPYTWAGRFADGRGT
                     NPEELVGAAHAGCYSMFLSAILTRNNVPPTRIDTRATVHLGDGPTITRIVLDVEAVVP
                     GVDESAFAAYAQEAKEKCPVSKALAAVEEIVLNAKLVG"
     gene            7421..>7667
                     /locus_tag="PLC06_09050"
     CDS             7421..>7667
                     /locus_tag="PLC06_09050"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRO90632.1"
                     /translation="MINRIRRNHGLEHATIHILSERHKRFSAQGNSDHRGFHLNIYGD
                     VTEDEVRAAVAEAHRRLTAGEHQLAVHPNCGTVLVTTA"
BASE COUNT         1444 a         2316 c         2357 g         1550 t
ORIGIN      
        1 ggcgtgttgg cgctgatgaa atcgcgcatc gtcatgcatc gctgcggcac ggccgcgagc
       61 atcgctttat cttccgtgac gagcctggcc tcattcccga tagccacgac cagatattgg
      121 gcgtcgtagg cgctcaattg gtgacgggcg gccagttcaa gcgccataat ggggtctgcc
      181 gtcgattcat gccctttaaa caacgttaca gccgacagcc aaagcgcgac ggccgtttcc
      241 agatcgatcc cgccatgccg gacgtaggtc gccaggatat tcagaaattc gtctcgccac
      301 agatcgggga caatccagtc agcatccagt tcccgcacgg tgcgcgcttc ggtcgttttt
      361 tcgccggtga tgaacaggta ggcgatgaca ttgacatcaa cgacgatcac gatcgccctt
      421 cttcacgagc cttttgcagc cattcgtcgt cgatgaggaa cgcgccgcgg attggctgcg
      481 gcaacggttg cggctcttct tcagccagcg accgctccag cagaatgatg atctcctggt
      541 tcatgcttcg ccggtttgct cgcgcgctct gctttagcct ggcgtgaagt tcatccggca
      601 tttggcggat caataagtcg gtcatgattt actcaatatc attatgatat cataatcata
      661 tcattctggg ccgattcgtc aacccttact caatctcaat cttcgtgatc gcctcgaaat
      721 cgagcgcttt ttcgctgccc agcacggcgt ggagcgccag ttgggcgaac tggaagaacg
      781 cttgcaggtc gccgatgcgg tcgaggtagt agcgggccag ttgctgctcc tcggccgaca
      841 ggtcgcactc ggccgcctgg agcttctcga tactctcccc cagcacgtgc agcgccagat
      901 gcacctcgcg ttgttcgcgg ctttccagca cgttacggat gacctgccac atgtcggatt
      961 cggccttgta gaacttgcgc cgctcaccgc gcacccacac ttccttggcc atgccccagc
     1021 gctccagccc gcgcatgttc atgctgaccg agcctttgct gatctccagc gtgtcaccca
     1081 aatcatcgag cgagagcggg ccggggctga ggagcagcgt tccatagagt cgccccatca
     1141 catcgctgaa accgaagaaa cgggccagcc ggccgagacc ggtgacggtg ctttcattga
     1201 cggcggttaa tgtctcgtgc atggttccct gcattcaata tgttttgaat gcagggagca
     1261 tagcataggg aaggggtggg gagcaagacg atcagtggcc cgtatgtcag caggtcagtg
     1321 gatcagtaga tcagtggggt agtggcggga tggggagatc gtgggacagt gggcagtaga
     1381 tcagttgatc agtaggtcag tggtcagtgg tctgtcgtct gtcgaccacc gaatgcccca
     1441 gcgggccatg gccggcgccg agccgccagg gcgcgccgcg gcggatagcg gcatgggtga
     1501 aggcttgtgc tcggcggatg gcgtcgaggg gggtgtgccc catcgcccgg taggcgcaca
     1561 cggccgccga tagcgtgtcc ccgctgccgt gggtgttgat cgtgtcaatg cgcggctgcg
     1621 gcagttcggt ccaactctgg ccgtcccacc aggcgtcgct gatcgcgtcg ccgcggcgaa
     1681 ggctcttgat tagccattga gcaactgatg gctgttctct atcggatgcc ggatgatcac
     1741 tacccggcac ttctgtcggc ggtctttggt catcggccgg cggcccaccc gcgaacgttg
     1801 actccagcga ccaccccagc agcgccgctt cggccgcgtt cggcgtcacc aaatcggcca
     1861 gagggaagag ccattcgcgg taggcggcgg caacttcggg gccgaacatg gcctcgccgc
     1921 gatggttgac gaggacgggg tcgatgatga tggccgggcg agggtgtaac ctctcacgcc
     1981 agtggttcag tcgctcggcc acgatcttga tggtgtcggc gcggccgagg aagccggtct
     2041 tgatgcccgc cgcgccataa tccgacaaca ccgcgtcgat ctgtgcggcc acgaaggcgg
     2101 gcggcagata gtggacggcg gcgacgcgct cgctgttctg ggcggtgacg gcggtgatgg
     2161 cgctcatgcc ataaacgcga acgccgtttt cgctaagggc ggcccatgtc ttcaggtcgg
     2221 cctgcaagcc ggccgcgccg ccggaatcgg agccggcgat cgtaagcagg gggagggacg
     2281 aattcagcgt catctgatga ttgagtatag ccggacttca actgctgcat caatgacaag
     2341 ttgccggcac aggctgtttc gcacccgacg agatgcgaca ctgcccgaag accgctgaat
     2401 ttttcactca ttctttctct gcggcgattg gctcgatggt atgatgcgga taccatagag
     2461 gccgatcaat gatgacaact ccagagaaac aagctcaacc ttccccggat atgctttccc
     2521 ccagcggttc ctatgccggt gaaattcgtc gtatcggtca gctcgtcggc cgcggccaat
     2581 ggcgaactgc cctgcaccgt gccgatagcc tgggagccaa cttctggaac tttgaggtac
     2641 gcgggggtag ccgccgcgtg cagtgcccgt gctgcggctg ggaggggcct gcgtttgtgt
     2701 cgttgagcaa ctggcgcgcc gtgcagcaca attcgcgctg ccccaactgc gattcccgct
     2761 cgcgccaccg cggtctgacg attctgctgc ccaggctgct tgccgccatg cccgacggcc
     2821 aggcgctggt cttcgcgccc gaggcggcat tgctggcgct ggttcggcag cacgcgcgcg
     2881 ccggtgtgga gacgaccgat tatctcatgg aggatgtcga tcatccgggc gaggatatcc
     2941 agcgcctgac atttcccgat gctcattacg gggcgctgat gtgcaaccat gtcctggagc
     3001 atatccccga cgaccgggcg gcgctggccg aatgtgcccg ggtcttgatg cccggcggcg
     3061 tggcggtgtt cacgattccc ggcgactacg accagacagc cacgcgctac tttgatttgc
     3121 ccgatcccaa cggccattta cgccattacg ggctggacgt gctcgagaag atgcgggcgg
     3181 tattcagccg ggtggaggcg gtcgatatga gcctggggac gaacccgcgc caacatattc
     3241 atccgggaga tatggcgttt gtgtgctaca agtagagggg taaggcgcgc tgcgcggggt
     3301 gatacccagc cgcccgaagt cgccggtcgt tcatgaccgc cagacctcat tgatgatttt
     3361 gcccacggcc gcggggaacg accaggcggg gcggccgtcg aactcatgcc gaacgaccag
     3421 cccttgcgac tccaggtggt tcatcgcgat cagcaccgtt gagcgcgtca gatcgcatct
     3481 gaggctgatt ttgcgggtga cgacgggcag ctccattagc cccatcgtca atacgatctt
     3541 gagctggttg cccgtcagct cgcgcaccaa tgtctccaat tgcaagctgt ccggctcatc
     3601 ctgtgggtcc attttctcct tcctcctgaa agcggtcagg tcgacgccga gaggcaacgc
     3661 gtcatcgggc agattaaacg cctgtagcgt ctctcgataa agcgggctgt tcgcgaaata
     3721 aagcgccgcg ctgaactgat gaaccggatc gtcgccgaaa taatccacga ccgcctgccg
     3781 caggatgccc agcaatagcc ggtgcgaggg cggcagcgcg ggagaaattt ccagatcgag
     3841 caaatagtgc aaccagacgt ttcgttgcgc ctcgtctgcg ggtggatcgt cgttcatggc
     3901 cctccggttc acatccataa tgaagtcggg ccgattgtat tagaaaatag aacgttagtg
     3961 ctatttgatt ttcgacactt tgctgaaaat cgttacaata tccgcctatg gtttcctatc
     4021 gaatccggcg cttgccggca gatgacgaac cggggcggcg ctatcatcta ttcgaccgcg
     4081 ccgatcagct tttgctggtg gccgaccagg gcagtgcgtg gctgccggcc ggcaacgtgg
     4141 cccaggtgcg ctttgcccgg cccggtggcg agcaagtggc ctcgatggat ctgccgggcc
     4201 cggccggcgg ccgaccggaa agaaaacaga actacgccat catctacgag cacgccgtct
     4261 acgccctgtt gacggccaag gacctgtcca gcggcacggg gacattgact caggcgctcg
     4321 tgccggcaaa gtttgaccgg ctgatcatcg aagttgaagg ttacaaatgg ctgggattat
     4381 gctggaccgg cggcgacaac ggccgtttgc tggttctgta tgacatggag acgaccgggc
     4441 tttcatcgtc agtggacccc gagtcggccg atctgccgga gccgatcggc ttgctggaag
     4501 tgggcgcggg cgattatgac ttcgacctga cgctgcccgc gggtcgcctg acgcaagggc
     4561 cgctgctggg gctggcgctg gcctacctcg tcgacggatt cctatagtcg cgaccgagct
     4621 aacgatcctc gaaccgtccc ggatagccgc ggatcgtagc ccgcagcaca tcggtgccgt
     4681 cggggccgat gacgcggccg gccaaccgta cccacccttt gtcgctgact tcgacgactt
     4741 ccgattcggc cgtcaccgta tcgccgatgt agaccgggcc gatgaactcg aacgacatgt
     4801 cggtggccag aaaagcccac aacccgccga gatgagtcag caggccggcc gtcagcaggc
     4861 cggggacgat gcggcggccg aaacgggtct gcgcgacgta ggcgtcatcg gtgtgatatg
     4921 gatttacgtc ccaggtgatg ccgatgaaga tcgccgcgtc ggcctcggtc agggttcggc
     4981 tgaacgcgac tttctcgccg gcgcgaacgg cgcgggggat gcggtgggga agaagaggag
     5041 ataccataat cagctaacca tcaattttgt gggccacgtt cttgccgacg tgggccgttt
     5101 tttcgtgccc gatgggcagc tgcatccaca ccggcgcagc ctcaccggta atgagtcgcg
     5161 cgccttgccg ccgggtgagg tagttccagc cccattgggt catcactttc aacttgttgt
     5221 cgaactcgat caggaagttg atgtggacga agacccagat gagccaggcc gggaatccgg
     5281 caaagtgcag gttgccgata tcggctaccg ccttgttgcg accaatcacc gccagcatcc
     5341 ctttgtcctt atatacgaac ggtcgtatct ccttgtcgaa gcgacggcga cgcagcacgt
     5401 cggccacata cttgcccatc tgcatggcca cctgggccac gcccggcagc ggcttgcccg
     5461 tctggtggtc atagtgagcc aggtcgccga tgacgaagat gtcgggatgg ccggcaatgg
     5521 tcaggtcggg acgcacgatg acgcggccga cacggtccag ctcggccccc gcgcggtggg
     5581 ccagcacgtc actgagcggc gacgccttca cccccgcacc ccacaatatg gtgaaggtcg
     5641 gaatcaatgt ctgttcaccg ctctttgtgt cctttaccag aacgtaatca tcagcgatct
     5701 ccgtcaccag cttgttggtc tgaacggtga cccccagccc gcgcagcgca tcggcggccg
     5761 tggccgagag ttcttcggga taggcggaca gaagccgatc ctgtccttca atcaggtcga
     5821 tctgcgcgtc ggccgtgtcg atggcgcgga actcctcacg catggtatgc gtagccagct
     5881 cgcccagcgc cccggccagt tcgacgccgg tcggcccgcc gccgacgatg acgaaggtca
     5941 tcagcgcgcg gcgacgctcg gggtcggtct cgcgctcggc ggcctcgaaa gccagcagga
     6001 tgcgccggcg aatcttgacc gcgtcctcga tggtcttcaa ccccggcgcg agcctttccc
     6061 attcgccgtc gtggccgaaa taatggtggc tggcgcccgt ggcgacgatc agcgcgtcat
     6121 aggcgatctc gtccccgtct tccaggatga cgaccttgcg ctcggggtca atgtcgatca
     6181 cctggccggt cagaaactgg gcgttcttct gatccttcag cgccgcccgc agcggcgaag
     6241 cgatatcttc gggcgacagc ccgccggtcg ccacctgata gagcaatggc tggaacaggt
     6301 ggaaattgcg tcggtcgatg agcgttacgt cgaccggtgc atgcttcatc gccttgatcg
     6361 cgtacaggcc gccgaagccg ccgcccacga tgacgatgtg ttcttttcgc ttgttcatct
     6421 ctcttcaata tcctcgataa atcgctttgt ccgacagggc gattggataa cgccctgaac
     6481 tattttcgcc ggatcattcg atttctcgct attgtaaccc caattgtgaa atttgttaca
     6541 aacattagac gcccttaacc ccgattttct ttccccatca tggaacatgt gcaaatgaat
     6601 gcgatggagt ggcggaacaa cgcggccgac gttgtttccc cgtcaaacct gcggttgctg
     6661 catccaataa agagaaggcc tctgaaagaa tcagatgaaa gctcattatc gacagggcct
     6721 tcggtctggc atcgccgggt aatattgact aaactacctt gaggctggac gggatcatga
     6781 gaacacgtgc ctgtcccgac gacgattcaa actaacaaag gagacttatt cttatggcta
     6841 ttcgtcatgc agatgcccaa tgggagggca cgctgaaaga aggcagcggc tatctgaagc
     6901 tggagagcgg tgtttatgcc ggtccttaca cctgggccgg gcgattcgcc gacggccgcg
     6961 gcaccaaccc cgaggagctt gttggcgcgg cccatgccgg ctgctacagc atgttcctgt
     7021 ccgccatcct gacccgcaac aacgtccccc cgacgcgcat cgacacccgc gccacggtcc
     7081 atctgggcga cggcccgacc atcacccgca tcgtgctgga tgtggaagcc gtggttcccg
     7141 gcgtggatga gtcagctttt gcagcctatg cgcaggaagc caaggagaag tgccccgttt
     7201 ccaaggcgct ggcggccgtt gaggagatcg tgctcaacgc gaagctcgtc gggtagatcg
     7261 gcaaaaaccg aagaaaaaca gaaggccggc cccattcgag ccggcctttt tctttcgcgc
     7321 catccatact gtccacggcc ccttcccttt catgtgctta cccatagcgc ggttgaattg
     7381 tccggggggt tatgatagcc ttgtgacaat ggtcaccaac atgattaatc gcattcggcg
     7441 caaccacggg ctggaacacg ccactatcca cattttgtcc gagcggcaca agcggttcag
     7501 cgcccagggc aattccgatc atcgcggctt ccacctgaat atctatggcg atgtgacgga
     7561 ggacgaggtg cgcgcggccg tggccgaggc ccatcgtcga ttgacagcgg gcgagcatca
     7621 actggccgtc catcccaact gcggcacggt gctggtgaca acggccg
//
LOCUS       DAOMQG010000140         7658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4118_length_7658_cov_6.514008, whole genome shotgun sequence.
ACCESSION   DAOMQG010000140 DAOMQG010000000
VERSION     DAOMQG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_7658_cov_6.514008"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLC06_09055"
     CDS             complement(<1..728)
                     /locus_tag="PLC06_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRO90633.1"
                     /translation="MDSGPNDRRTQVASQFHRAHRQASLEVLLSRLSGHPVDLLSYND
                     VIDKLGIAGHSRLGVRQIPLDAIVGSVGRYQDFTRTFLPRSKEDEERWVSVASAATSV
                     ADLPPIEVYKIGDVYFVLDGNHRVSLARGQALEAIAANVIEVRTRVPMPPGGKPDDLI
                     VAAEMGAFLDYTHLDELRSEADFRVSVPGQYRHLQSHIEAYRFACELDKNCELPPNEA
                     TERWYDEVYLPLVEAIREQGILRY"
     gene            complement(887..2296)
                     /gene="dnaA"
                     /locus_tag="PLC06_09060"
     CDS             complement(887..2296)
                     /gene="dnaA"
                     /locus_tag="PLC06_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRO90634.1"
                     /translation="MNTGNLAPETVWKAALGELEMQMTRATFNTWLQGTRALSCVDGE
                     FVIGVRNDFAKDWLENRLYDLIVRTVSGVMGHPVEVRFVVWSDEIIAPRTAMVSQNGR
                     AEVVPSQPGPTPANGRHEAESPAEEGALNRRYTFSTFIVGANNRLAHAAALSVAENPG
                     QTYNPLFIYGGVGLGKTHLLHAIGHRCRQDGRRVRYISAETFTNDLVMAIRTKTTPDF
                     RERYRTVDVLLIDDIQFIAGKEGTQDEFFHTFNDLHSHGKQVIISSDRPPKSLATLEE
                     RLLSRFEWGLTADVQPPDEETRRAILLAKADDLNQYVPDEVIEFIAHNSRQNIRELEG
                     ALNKVVAYAGLTDRPIDLELVNLALADTLRRPERISVEQIIDIVCRYYNVTSDAIISS
                     SRKSTIAHPRQVAMYLSRTETDASLPQIGAQMGPRDHTTVLHGAEKIKSLVDIDPQLR
                     RDLLEIKAMLYERAAVITH"
     gene            complement(2876..3442)
                     /locus_tag="PLC06_09065"
     CDS             complement(2876..3442)
                     /locus_tag="PLC06_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90635.1"
                     /translation="MRRRAILIPLLCGLLLALFVAARLSAHGIGKPQVVNERVGPYLI
                     SVWTDPDPLRADETHVVVGVTNPDTREPVVGGIEVTVALVSAADPAVVVTQTAGTDSV
                     NQLLYAAEFNDRVAAGRWRVVVTVTGAQGVVEAPGFDVDIDPARGFGMLRLGLGGLAA
                     IVAVWFLGSIRDESPARARRKRPAPKPR"
     gene            complement(3444..4892)
                     /locus_tag="PLC06_09070"
     CDS             complement(3444..4892)
                     /locus_tag="PLC06_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FesM"
                     /protein_id="HRO90636.1"
                     /translation="MIPLREITASGETDALYRPPGRDLLRAPLIGRLFRHRWGRLFMQ
                     VPLLLVALLLVYDGFTGSPIPPQNTATVAVWVHYRGFIMLALLFFGNLFCMACPFTIP
                     RTIALRLSRRGRRWPRALRNKWLAVAVFVAFLFTYEWLDLWASPALTAWVIIGYFLSA
                     FVLEALFAESPFCKYICPLGTFNFAVSTLSPARITVRSHDVCRDCTGKECVNGSADVL
                     GCGTELFAPVMRSNLDCILCLDCARACPYDNVALMTRPRDSELVDNAWPRRWDVAFLM
                     LVFAFAGPGNAFGMVPPYYDLQLWLANTLGTNNEFLLLALTFGLMLLALPAVVGLGAA
                     AISRALSGREEPLRVSFSRYAPAFMPLAVAVWAAHYGFHFATGALTIIPAFQTFLTDH
                     GLNWLGAPNWQLGTILPGGWILPLQTLAVLAGFAGSLYVGTRIGRRDFGSPPAAMRAM
                     LPWVVVFIGLAAAALVLFNLPMEMRGAMLPGG"
     gene            complement(4898..5332)
                     /locus_tag="PLC06_09075"
     CDS             complement(4898..5332)
                     /locus_tag="PLC06_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRO90637.1"
                     /translation="MLPFRISARHGRLLIIVGLLLGLAACRQAAGGVPLGSPDDTADI
                     SLTLAPAAGAQPLPLEPLTWEIALADAAGAAIEGAAVIVRGDMSHAGMAPVEATAADA
                     GGGLYRADFAWTMAGDWIVTVTATLPDGRVKSQTFNYTVTAR"
     gene            complement(5393..6064)
                     /locus_tag="PLC06_09080"
     CDS             complement(5393..6064)
                     /locus_tag="PLC06_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRO90638.1"
                     /translation="MTATTTAESPSTGAGATPARPLIPWLIAAAVLLLAGGLLGYWLG
                     RAPIPAADSVEVGFARDMSIHHEQAVAMAALVYDRSDNDAVRSLAFDILTTQQGQIGI
                     MTGWLDAWGLPLTSAGPRMGWMGMPMTGLMPGMATAEQMATLRAATGIEADTLFLQLM
                     IPHHRSGMEMAQYIADRTRIESVRRFTAGMAAAQGLEITYMEELLKGIGAEPPPADAG
                     SHNHG"
     gene            complement(6267..6989)
                     /locus_tag="PLC06_09085"
     CDS             complement(6267..6989)
                     /locus_tag="PLC06_09085"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRO90639.1"
                     /translation="MGFDETSVQVTSYSNDGGIDVRGVMNAGDVTTVTASVQVKRWKT
                     NVPVKVVRELRGSLTTHEQGIIITTSDYTADAQKEARAIGKAPISLVNGEKLIELLVK
                     HSIGVNKKPYTVISLDEEWWNESNDALVPDFDEPSLIVEKVDVTRTAADVSFPLTVRA
                     LNKPDVFAELLSREGEMLFQDERFKKPSAPGMIASGWKSANGWTYWLYQDPESGEWRP
                     IAELRRPANTPKHISEASPPRR"
     gene            complement(6992..7345)
                     /locus_tag="PLC06_09090"
     CDS             complement(6992..7345)
                     /locus_tag="PLC06_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRO90640.1"
                     /translation="MDFLTAAAMVLEQVGTPLHYKEIAQRALLLPQFTPLGQTPEATM
                     GSRLYVDTKKDDSRFERIGRGLFALKPKSRQDEIARRVDEINDQARRALHKRLRVMPA
                     DRFEAFIGELLIAIG"
BASE COUNT         1443 a         2373 c         2459 g         1383 t
ORIGIN      
        1 aaatagcgca atatcccttg ctcgcggatc gcctcgacca gcggcagata gacctcgtcg
       61 taccagcgct ccgtcgcctc gttgggcggc agctcgcaat tcttgtccag ctcgcaggcg
      121 aaacgatagg cctcgatgtg ggattgcaag tggcgatact ggccgggaac gctcacccgg
      181 aagtcggctt ccgatcgcag ttcgtccaga tgggtgtaat cgaggaacgc gcccatctca
      241 gcggcgacga tcagatcatc gggcttgccg ccgggcggca tgggcacgcg ggtgcgcact
      301 tcgatgacgt tggcggcgat cgcttccagc gcctggccgc gagccagact cacccggtgg
      361 ttgccgtcga ggacgaagta gacgtcaccg atcttgtaga cttcgatggg gggcaggtcg
      421 gccacactcg tcgccgccga ggcgacgctc acccagcgct cctcgtcttc cttgctgcgc
      481 ggcaaaaagg tgcgggtgaa atcctgataa cggccgacgc tgccgacgat ggcgtcgagg
      541 gggatttgcc ggacgccaag gcgggaatgc ccggcgatgc ccagcttgtc gatgacgtca
      601 ttgtaggaca gcagatcaac aggatggccc gacaggcgcg acagcagcac ttccagcgac
      661 gcctggcgat gggcgcgatg aaattgtgag gcaacctgtg tgcggcgatc gttcggtccc
      721 gaatccattg ggatctaatg ataaacgatc catgcattat cgggcaatgt tgtagagcaa
      781 gctgccggct tgcgggccgg ggagaagatt caaccctgac aggtgggcga cgacaaattt
      841 cgtggccggc agagtgctct ttgtcgccca cctgtcaggt ttttaatcaa tgcgtgatca
      901 ccgccgcgcg ctcatagagc atggccttga tctccagcag gtcgcggcgg agttgcgggt
      961 cgatgtcgac cagcgacttg atcttctccg cgccgtggag gacggtggtg tggtcgcgcg
     1021 gccccatctg cgcgccgatc tgcggcaggg aggcatcggt ctccgtgcgc gagaggtaca
     1081 tcgccacctg gcgcgggtgg gcgatggtgc tcttgcggct gctgctgatg atggcgtccg
     1141 acgtcacatt ataatagcgg cagacgatgt cgatgatctg ctccacgctg atgcgctccg
     1201 gacggcgcag ggtatcggcc aaggccaggt tcaccagctc caggtcgatg gggcggtcgg
     1261 ttagcccggc ataggcgacc accttgttca gcgccccttc cagctcgcga atgttttgcc
     1321 gcgagttgtg ggcgatgaat tcgatcacct cgtcggggac atactggttc agatcgtcgg
     1381 ccttggccag caaaatagcg cggcgggtct cctcgtccgg cggctggacg tcggccgtca
     1441 ggccccattc gaagcgcgag agcaagcgtt cttccagcgt ggccagcgat ttgggcggcc
     1501 gatcgctgga gatgatcacc tgcttgccgt ggctgtgcag gtcgttgaag gtatggaaaa
     1561 actcgtcctg cgtgccttcc ttgccggcga tgaactggat gtcgtcgatt agcaggacgt
     1621 cgaccgtccg gtagcgctcg cggaagtcgg gcgtcgtctt ggtgcggatg gccatgacga
     1681 ggtcgttggt gaaggtttcg gccgagatgt agcgcacgcg gcggccgtcc tggcggcagc
     1741 ggtggccgat ggcgtgcaga aggtgggttt tgcccaagcc cacgccgcca tagatgaaca
     1801 gcgggttgta ggtctggccg gggttctcgg ccacggagag cgcggcggcg tgggccagcc
     1861 ggttgttggc cccgacgatg aaggtggaga aggtgtagcg acgattgagc gccccttcct
     1921 cggccggcga ctcggcctcg tggcggccgt tggcgggcgt cgggccgggc tgcgacggga
     1981 ccacttccgc gcggccgttc tggctcacca tcgccgtcct gggggcgatg atctcgtcgg
     2041 accagacgac aaagcgcact tccacggggt gacccatgac ccccgacacc gtgcgaacga
     2101 tcagatcata gagacgattc tcaagccaat ccttggcaaa atcgttccgt actccgatga
     2161 cgaactcgcc gtcgacgcac gataacgccc gagtgccctg cagccaggtg ttgaacgttg
     2221 cgcgcgtcat ttgcatctcg agttcgccca acgctgcctt ccacaccgtc tcgggcgcca
     2281 gattaccagt attcattatc ttccctcggc aagcggccaa caggcgtact acatcctcgg
     2341 tgaacccgct tatgttgaaa tcggacaaac aatcagaggc gcgaaatccc ttgagtgggt
     2401 ggacggatcc ctcttccgca ttcacccttg cagttccatg acagttccgc acgggcgtgg
     2461 gtctgtcgtg cgaaccgggc gacgagattt cttcttcggc gctgtctcaa tggtgaagcc
     2521 attctagcat accccggcag gcccgacaat gctcatagtc gtgatctgtc ttaaaacctg
     2581 cggatttttt aggaatcgac aatgttaaag aaatgtagac cttaaaaaat tggttgtgag
     2641 gttctcgcgt catcccaaca attaggtgtg ccgccgtcat gataccccca tatgtgcccc
     2701 tagttgacca taacaggggg tagtaatccc ccctattgga tatccttggc gggcagggaa
     2761 atcggccgcg ctaatgtcaa tctgctgcat gacgacatta acggcgcaaa acagggcgaa
     2821 attgttgcaa aatggaccga tctgtggtgg gctgtttggg gcagggagac ggccctcagc
     2881 gcggttttgg cgcggggcgt ttgcggcggg cgcgggccgg tgattcatca cggatcgacc
     2941 ccaaaaacca gaccgcgacg atggccgcca agccgcccag acccagccgc agcatgccga
     3001 aaccgcgcgc cggatcgatg tcgacgtcga agcccggtgc ctcgaccacc ccctgcgccc
     3061 cggtcacagt gaccacgacc cgccagcgcc cggccgccac gcggtcgttg aactcggccg
     3121 catagagcag ctggttgacg ctgtcggtcc ccgccgtttg ggtaacgacc accgccgggt
     3181 cggccgccga gacgagggcg accgtcacct cgatgccgcc gacgaccggt tcgcgggtgt
     3241 cggggttcgt cacgccgacg accacatggg tttcgtcggc gcgcaggggg tcggggtcgg
     3301 tccagacgga gatcaggtac ggcccgacgc gttcgttcac cacctgcggc ttgccgatgc
     3361 cgtgggcgct caggcgcgcc gccacgaaca acgccaacag caggccgcag agcaggggga
     3421 taaggattgc gcgccgcctc atctcagccc cccggcagca tcgccccgcg catctccatg
     3481 ggcaggttga acaggaccag cgcggccgcg gccagtccga tgaacacgac cacccacggc
     3541 agcatggccc gcatggccgc gggcggcgag ccaaaatcgc ggcggccgat gcgcgtgccc
     3601 acatagaggc tgcccgcaaa cccggccagc accgccagcg tctgcaatgg caaaatccag
     3661 ccgccgggca ggatcgtccc caattgccag ttgggcgcgc ccagccagtt caggccgtgg
     3721 tcggtgagga acgtctggaa cgcggggatg atcgtcagcg cgccggtggc gaaatggaaa
     3781 ccgtagtggg cggcccagac ggcgacggcc agcggcatga acgccggcgc gtagcgactg
     3841 aaggatacgc gcagcggctc ctcgcggccc gacagcgccc ggctgatcgc cgccgcgccc
     3901 agtccgacga cggccggcag cgccagcagc atcaggccga acgtcagcgc gagcagcagg
     3961 aactcattgt tcgtgcccag cgtattggcc agccacagtt gcaggtcgta gtagggggga
     4021 accatgccga aggcgttgcc cggcccggcg aaggcgaaca ccagcatcaa aaacgccacg
     4081 tcccagcggc gcggccaggc gttatcgacc agctcgctat cacgcggccg cgtcatcagg
     4141 gccacgttgt cataggggca ggcccgcgcg caatcgaggc acaggatgca atcgaggttg
     4201 ctcctcatca ccggcgcgaa cagttcggtg ccgcagccca acacatcggc cgagccgttg
     4261 acgcactcct tgcccgtgca gtcgcggcaa acgtcgtggc tgcgcacggt gatgcgcgcc
     4321 ggcgacaggg tgctgacggc gaagttgaac gtccccagcg ggcagatgta tttgcagaag
     4381 ggggactcgg caaacagcgc ctcgagcacg aacgcgctca ggaaatagcc gatgatcacc
     4441 cacgccgtca gcgccgggct tgcccacaga tcgagccatt cgtaggtgaa taaaaaggcc
     4501 acgaagacgg ccacggccag ccatttgttg cgcagcgccc gcggccagcg ccgcccgcgc
     4561 cggctcagcc gcagggcgat ggtgcgcggg atggtgaacg ggcaggccat gcagaacagg
     4621 ttgccgaaga acagcagggc cagcatgatg aacccgcgat aatgcaccca cacggcgacg
     4681 gtggccgtgt tctgcggcgg gatgggcgac ccggtgaagc cgtcgtagac gagcaggagc
     4741 gccaccagca gcagggggac ctgcatgaac agccgccccc agcgatggcg aaacaggcgg
     4801 ccgatcagcg gcgcgcgcag caggtcgcgt cccggcggcc gatagagcgc gtcggtctcg
     4861 ccggaggcgg tgatttcgcg tagcgggatc atgttactca acgggcagtc accgtatagt
     4921 tgaaggtctg cgacttgacc cggccgtcgg gcagcgtggc cgtcacggtg acgatccagt
     4981 cgccggccat cgtccacgcg aagtcggcgc gatagagtcc gccgccggcg tcggccgccg
     5041 tggcctcgac cggggccatg ccggcatggc tcatgtcgcc gcgcacgatg acggccgcgc
     5101 cctcgatggc cgcgccggct gcatcggcca gcgcaatctc ccaggtgagc ggctccaggg
     5161 gcaggggttg cgcgccggcg gccggggcca gtgtcaggct gatgtcggcc gtgtcgtcgg
     5221 gcgaaccgag cggcacgccc ccggcggcct gacggcaggc cgccagaccc agcagcaagc
     5281 caacgattat gagcaatcgc ccgtggcggg cggaaattcg aaacggcagc atatggctcc
     5341 tcagcgctcg cgaatgcccc ctaaggcggc gaggcaagcg tgattgtacc agctatccgt
     5401 gattatgact gcctgcgtcc gcgggtgggg gttccgcgcc gatccccttg agcagctctt
     5461 ccatataggt gatctccagc ccttgcgcgg cagccatccc ggcggtgaag cggcgaaccg
     5521 actcgatccg ggtgcggtcg gcgatgtatt gggccatctc catgccggat cggtggtggg
     5581 ggatcatcag ctgcaggaag agggtatcgg cttcgatgcc ggtggcggcg cgcagggtgg
     5641 ccatctgttc ggccgtggcc ataccgggca tgagaccggt catcggcatg cccatccagc
     5701 ccatgcgcgg cccggcggaa gtcaacggca agccccaggc gtcgagccag ccggtcatga
     5761 tgccgatctg gccctgctgc gtggtcagga tgtcgaaggc cagcgagcgc acggcgtcgt
     5821 tgtcggagcg gtcatagacc agcgccgcca tggcgaccgc ctgctcatgg tggatgctca
     5881 tgtcgcgggc gaagccgacc tcgaccgaat cggccgccgg aatgggggcg cggccgagcc
     5941 aatagcccag caagccgccg gcgagcagca ggacggcagc ggcgatgagc catgggatga
     6001 gtgggcgggc gggtgtggcg ccggcacccg tagaaggact ttcagcggtg gtggttgctg
     6061 tcatagtcaa tgaatgatcc tttgttattt cgcatagcca atcggcagta caactactga
     6121 actcaagcgg cagcgaccga cgaaatcaaa ccttagcaca ccatcctgcc cagggcaatc
     6181 gcgtgtgacc aagtgtgcgg gttcagcatg cgcgggttca aacttgcggc cgggagacaa
     6241 aaccggttgg aaaccggttc ggggagctat cggcggggtg gggaggcttc ggaaatgtgt
     6301 ttcggtgtat tagcaggtcg tcgtaattcg gcaatcggcc gccattcgcc gctttcgggg
     6361 tcctgataaa gccaataagt ccaaccattg gccgacttcc agccggaggc tatcatccca
     6421 ggggctgaag gttttttaaa gcgctcatcc tggaaaagca tctccccttc ccgggacaac
     6481 aactcggcaa acacatctgg tttgtttagc gctctaacag tcagcggaaa tgatacatcc
     6541 gccgccgtcc gtgtcacatc aaccttttca actatcaacg aaggctcatc aaagtcaggc
     6601 accagggcat cattcgattc attccaccac tcttcgtcga gcgaaatcac cgtgtacggc
     6661 ttcttgttta ccccaatcga atgttttact agaagttcga tcaatttttc cccgttcacc
     6721 agacttatcg gcgccttgcc gatggcgcgg gcctcttttt gggcatctgc cgtgtaatcg
     6781 ctggtcgtga taatgatccc ttgttcatga gtggtcagcg atccacgcaa ctcgcgaacc
     6841 actttgaccg gcacgttagt tttccatcgt ttgacctgaa cggacgccgt gacggtcgtc
     6901 acgtcgccgg cattcatgac gcctcgtaca tcaatccccc cgtcattgct gtagctcgtc
     6961 acctgaaccg atgtttcatc gaacccgata gctacccgat agctattagc agttcaccga
     7021 tgaacgcctc aaagcggtcg gccggcataa cgcggagccg cttgtgcagc gcccgccggg
     7081 cctgatcatt gatctcatcc actcgtcgag cgatctcatc ctgccgtgac ttgggtttta
     7141 aggcgaataa tccccggcct atgcgctcaa accgcgaatc atccttcttg gtatccacgt
     7201 acaggcgcga acccatggtg gcctcaggcg tctgtcccaa aggcgtgaac tgcggcagca
     7261 acaaagcgcg ctgggcgatc tccttgtaat gaagcggcgt acccacttgc tccaggacca
     7321 ttgcggcggc tgtcagaaaa tccatgttgt tgttgcccca tgtggattgg cgtgcggaaa
     7381 tcttaacatt acacgacagg taggcaaggt gatatattcg gccgcggcgc gcacctcgtc
     7441 gcgagcctcc gcccgataga gcttgacctc gcgctcgccg gtgaagacct cgccgtggtc
     7501 ggccacgcgt taccgggatt tcatttgttt gttcgggggt tcggtactca tgggctatcg
     7561 gtatgataac ccccgcccct tgccgcgcaa cttaagccgc cacctcacaa aaccggcatc
     7621 ctctcgcctc ggcggccaca atcgcctgat cgctgctc
//
LOCUS       DAOMQG010000141         7654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4122_length_7654_cov_12.128043, whole genome shotgun sequence.
ACCESSION   DAOMQG010000141 DAOMQG010000000
VERSION     DAOMQG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4122_length_7654_cov_12.128043"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1382)
                     /gene="mtaB"
                     /locus_tag="PLC06_09095"
     CDS             complement(75..1382)
                     /gene="mtaB"
                     /locus_tag="PLC06_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRO90641.1"
                     /translation="MKIHLHSIGCRLNQAEIETMARQLQAGGHVIVGDAAEADAIILN
                     TCAVTAEAARDARHLTRRFHRANESAQIVLTGCYATLAPDVVAALPGVGRVVGNGDKE
                     RLPLLLDPALPVDAPLFDSEPIAREAQPGSFGRTRGFVKVQDGCDNRCTFCVTTIARG
                     AGRSRHLADVVTEVGRFAAAGYGEVVLTGVHLGSYGRDLGRPAGLRELVAALLAHTDI
                     PRIRLSSLEPWEIAEGFFELWDDPRLMPHLHLPLQSGSDRVLRRMARRTSRAAFRALV
                     DDARAHIPDLSVSTDVICGFPGETEDEFAETLEYVEAIGFSRIHAFSYSPRPGTAAAR
                     MPDQVPGPVKRDRTKQLIALGEQLSLAFHRRYEGTTRPVLWETATGAERDGLCWAGYT
                     DNYIRVAAVGPADLMRRETLTRLGDARGEGMSGVIVWMNQRYG"
     gene            complement(1549..1755)
                     /locus_tag="PLC06_09100"
                     /pseudo
     CDS             complement(1549..1755)
                     /locus_tag="PLC06_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
     gene            complement(2101..4071)
                     /locus_tag="PLC06_09105"
     CDS             complement(2101..4071)
                     /locus_tag="PLC06_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRO90642.1"
                     /translation="MMTDKFTFGGEIVWRPTPDTIERSNLTRFMRRHGIGSWDELMAR
                     STSDVAWFTDAVLEFLDVRFRVPYTQVVDLSDGIEWPRWCVGGRMNIVDNCVDKWAAD
                     PATRGRPALVWEGEEGATRTLTYGELAAEVNRCANALRGLGLGKGDAMGLFMPMTPEI
                     VIALLAIAKIGGVILPLFSGYGAGAVATRLADAGAKALFTADGFYRRGTVVPLKPTAD
                     EAAAAVPTVEHLIVLQRAGNEVTMQDGRDHWWHELVAPQSPVAETADTAAEELLMIIY
                     TSGTTGRPKGAVHTHCGFPVKAAQDMAFGMDTHPEDTIYWMTDMGWMMGPWLVFGATL
                     LGATFFIYDGAPDYPDVDRLWALAERHRLTQVGVSPTLIRALVPHGEAPVRRHDLSSV
                     RLFGSTGEPWNPDPWLWLFNVVGEGKRPIINYSGGTEISGGIVMGNPILPLKPAAFAA
                     PCPGMAADVVDADGNSVRNEVGELVIRAPWIGMTRGFWRDRQRYLDTYWSRWPGVWVH
                     GDWAAIDEDGLWYILGRSDDTIKIAGKRLGPAEVESVLVAHPAVREAAAIGVPDALKG
                     SALVCFCVLAPGVVPSDALAAELRELVADEIGKPLRPKAIRFVSDLPKTRNAKVMRRV
                     IRSAWLGEDPGDTSSLVNPGVIGELRGGSSDG"
     gene            complement(4513..4686)
                     /locus_tag="PLC06_09110"
                     /pseudo
     CDS             complement(4513..4686)
                     /locus_tag="PLC06_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4728..5297
                     /locus_tag="PLC06_09115"
     CDS             4728..5297
                     /locus_tag="PLC06_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO90643.1"
                     /translation="MPKARDYHLNEQEFKAIEAAIRSDKRPEVRQRCTTMRLLHFGHK
                     PKDVAQMQAVSIPTIYGWYNRWRQDGIEGLATKPRSGRPSKTGEEYLKAVEEALAKEP
                     RAFGYDFSLWTIERLRLHLEKETGTALSDGRLCILLKRHGYRYRRPKYDLGHLQDKDA
                     KAQAAELLDELKKRPQPTISNSSLWTKQR"
     gene            5279..5791
                     /locus_tag="PLC06_09120"
     CDS             5279..5791
                     /locus_tag="PLC06_09120"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRO90644.1"
                     /translation="MDETTLSLDPPLRACWMKIGQQKRVPATQIGSKRRWHILGGYNW
                     LKDTVAWSTAVTKNTATFIEFLEELLLRQYPSERIVLVMDNVSYHKSAAALAALSLFE
                     HRVAVIWLPPYCSDLSPIERFWRFLKDLACANHLEDKIEDVVGMAEKVMSHQNDPTYD
                     YRFHVSKDLR"
     gene            complement(5783..5974)
                     /locus_tag="PLC06_09125"
     CDS             complement(5783..5974)
                     /locus_tag="PLC06_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90645.1"
                     /translation="MYPSVIAKQPSSDIMTYQKDCTLPDSLLKQIAEQGLGILPELIR
                     IVINTAMQAEREQYLSNLS"
     gene            6041..7330
                     /locus_tag="PLC06_09130"
     CDS             6041..7330
                     /locus_tag="PLC06_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90646.1"
                     /translation="MKKDLNIRRLFLLACMVLLLSACVAPGSDITSQAGSPQPAVLTD
                     KVDEPESIVEVVFTPTPLSTPTDVPTQTPTPEPEPTQTPTPEATPIPPEVPFRVEDGI
                     VQKWTVNPVTNQEEWVPDESFPVLEPEPGRRTPVIESIGSVTYQINGEEVGYFSAISA
                     IYGKTEAIFINGEWTLASEINVKDFIAPVAPGEVEFANENWSDGRKLIESGSVEFLEK
                     FQQALIDGNPAYFRWYVGSADIFLNNVLAVASTRNYMLPVPPDGVPKLLLPSWDKTRY
                     STEFAIRTRNPHTEINTDINFGGLTVLIFTPDQIKNDPVLKVIVNGLPMNYLLSVQSS
                     ERGQYGIVVLPDGSLLWVHGEQMGQPNQPKDFKTTLLGERPENFSGITRAFAAAYLEA
                     MTRAEGESVRVFPLNPFDYGDPEMISGDGVGLQPFVE"
     gene            7391..7591
                     /locus_tag="PLC06_09135"
     CDS             7391..7591
                     /locus_tag="PLC06_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRO90647.1"
                     /translation="MHNSKTIAGVAFDLGLLPNTLHRWRDELRIAGDEAFPGRGQMKA
                     SDEEVRRLEREITRLRQERDIL"
BASE COUNT         1574 a         2287 c         2277 g         1516 t
ORIGIN      
        1 cgccccaact gtcgaaccgc aggccgatca ggtcgccgaa cgggttggga agagttgccg
       61 ggttttccag ttttttatcc atatcgttga ttcatccata caatcacccc gctcatcccc
      121 tcgccccgcg catcgcccag gcgcgtcaat gtctcccgcc gcatcaaatc ggccgggccg
      181 acggccgcca cgcgaatgta gttgtccgtg tatcccgccc agcacaaccc gtcgcgctcc
      241 gcgccggtgg ccgtttccca cagcaccggc cgcgtcgttc cctcgtagcg ccggtggaag
      301 gccaggctca gctgctcgcc cagagcgatg agctgcttcg tccggtcgcg cttgaccggc
      361 cccggaacct gatcaggcat ccgcgccgcg gccgtgcccg gtcgggggct atagctgaaa
      421 gcatggatgc gcgagaagcc gatggcttcg acatattcca gcgtctcggc gaactcgtcc
      481 tccgtctcgc cggggaaacc gcaaatcaca tcggtgctga cgctgagatc ggggatatgc
      541 gcgcgggcgt cgtcgaccag ggcgcggaag gcagcgcggc tggtgcggcg ggccatgcgc
      601 cgcagcaccc ggtcgctgcc cgattgcagc ggcagatgca gatggggcat cagccgcggg
      661 tcgtcccaca actcgaagaa cccttcggcg atctcccacg gctccagcga cgacaggcgg
      721 atgcgcggta tgtcggtgtg ggccagcagc gccgccacca gctcgcgcag ccccgccggg
      781 cggcccaggt cgcggccgta gctgcccaga tgcacgccgg tcagcaccac ctcgccatag
      841 cccgccgccg caaagcgtcc cacctcggtc accacgtcgg ccaggtgacg gctgcgtccc
      901 gcgccgcggg cgatggtcgt cacgcagaag gtgcagcgat tgtcgcagcc gtcctgcacc
      961 ttgacgaacc cccgtgtgcg gccgaaactg cccggctgcg cctcgcgggc gatcggctcg
     1021 ctgtcgaaca gcggcgcgtc aacgggcagc gccgggtcga gcagcagcgg cagccgctcc
     1081 ttgtcgccat tgcccaccac acggccgacg ccgggcagcg ccgccaccac gtccggcgcg
     1141 agggtggcat agcagccggt caggacgatc tgcgccgact cattggcccg gtggaagcgc
     1201 cgggtcaggt ggcgggcatc gcgggccgcc tcggccgtca cggcgcaggt gttgaggatg
     1261 atggcgtcgg cctcggccgc gtcgccgacg atgacatggc cgcccgcctg gagctgccgg
     1321 gccatcgtct cgatctcggc ctggttcagc cggcagccga tgctatggag atggattttc
     1381 acgatttcag tagtctatgc tactgaccgg cggccgcaaa caacttttcc attgcaatat
     1441 cggcgatgtg ccggcggctt atcggtcgcg atcttcacgc aaaagcgttg tgctgtcggg
     1501 tgcgccgacc gccgcgggtg tgaagcgacg acgcggtgcg taatggcgta cgtccaccaa
     1561 tgctccggca tcagccatag tctcggcatc ctcaagcagc cgcgccgtta catcctcatc
     1621 gacataggct tcgccgcagt tcggacaaac ctgagcaggc acctgcttga tcaccagggt
     1681 gagactgtct cgctcaagcg tgacggtcgt gaaaccggga attgtttcgt cttgccggca
     1741 gatgacacat ttcatgttgc tctctcacga aaatcggctg accattgaat cggatcgggt
     1801 tcatagacgg tgatgacgat tatctcatca tcagccttgt tttcggcgat gacgatatgc
     1861 aagggtcgaa cgcctcgcca gcccagtagt attaaacgac tgggataagg ggtatcattt
     1921 ggatacgatc gtttcgcccg tcgtcacgat atgctgaacg tcagctttcg tgacatagcg
     1981 ttggaacatt cgttgaatcg cgtggactcg aaagcttagt ttcatgcttg ccgtcgtgga
     2041 aacattctac catagatgca ggaatttggt tttaactatc atacgagtcg aacagtcggc
     2101 ttaaccgtcg gacgaaccac cgcgcaactc gccgataacc cccggattca ccagactcga
     2161 tgtatccccc ggatcctcgc ccagccaggc gctgcggatg acgcggcgca tgaccttggc
     2221 gttgcgcgtc ttgggcaggt cgctgacgaa gcggatcgcc ttgggccgca gcggcttgcc
     2281 gatctcgtcg gccaccagtt cgcgcagttc ggccgccagc gcgtcggacg ggacgacccc
     2341 cggcgcgagg acgcagaagc agaccagcgc gctgcctttc agcgcgtcgg gcacgccgat
     2401 ggccgccgcc tcgcgcaccg ccgggtgggc caccagcacc gactccacct cggccgggcc
     2461 aagccgcttg ccggcgatct tgatcgtgtc gtcggagcgg ccgaggatat accacaggcc
     2521 gtcctcgtcg atggccgccc agtcgccatg aacccacacc cccggccaac gcgaccagta
     2581 ggtgtcgaga tagcgctgcc ggtcgcgcca gaagccgcgc gtcatgccga tccacggcgc
     2641 gcggatgacc agctcgccga cctcgttacg gacggagttg ccgtcggcgt cgaccacgtc
     2701 ggcggccatg ccgggacagg gggcggcgaa cgccgccggc ttcaggggca ggatagggtt
     2761 gcccatgacg atgccgccgc tgatctccgt gccgccggag tagttgatga tcggccgctt
     2821 tccctcgccc acgacgttga acagccacag ccagggatcg gggttccacg gctcgccggt
     2881 gctgccgaag aggcgcacgc tcgacaggtc atgccgtcgc accggcgctt cgccatgggg
     2941 gaccagggcg cggatgagcg tcggcgacac gcccacctgg gtcagccggt ggcgctcggc
     3001 cagcgcccac aggcgatcca cgtcggggta gtcgggcgcg ccgtcgtaga tgaagaacgt
     3061 cgcccccagc agcgtggccc cgaacaccag ccacggcccc atcatccagc ccatgtcggt
     3121 catccagtag atcgtgtctt cgggatgggt gtccatcccg aaggccatat cctgggccgc
     3181 cttcaccggg aagccgcaat gggtgtggac ggctcccttc ggccgcccgg tcgtgccgct
     3241 ggtgtagatg atcatcagca actcttcggc ggcggtgtcg gccgtttcgg ccacgggcga
     3301 ctgcggcgcg accagctcgt gccaccagtg gtcgcgcccg tcctgcatgg tcacctcgtt
     3361 gcccgcccgt tgcaggacga tgagatgctc gactgtgggg acggcggcgg ccgcctcgtc
     3421 ggccgtcggc ttcaagggca cgaccgtccc ccggcgatag aagccgtcgg cggtgaagag
     3481 cgccttcgcc ccggcgtcgg ccagccgcgt ggccaccgcg cccgcgccat agccggaaaa
     3541 cagcggcagg atgacgccgc cgatcttggc gatggccagc agggcaatga cgatctccgg
     3601 cgtcatgggc atgaacagcc ccatggcgtc gcccttgccc aggccgagac cgcgcagggc
     3661 gttggcgcag cggttgacct cggccgccag ctcgccgtag gtgagcgtcc gcgtcgcccc
     3721 ctcttcacct tcccagacga gcgccggacg gccgcgcgtc gccgggtcgg ccgcccattt
     3781 gtcgacgcag ttatcgacga tgttcatccg cccgcccacg caccagcggg gccactcgat
     3841 gccgtcggac agatcgacga cctgcgtata gggcacgcgg aagcgaacgt cgagaaactc
     3901 cagcacggcg tcggtgaacc aggccacgtc ggacgtggaa cgagccatca gttcgtccca
     3961 cgagccgatg ccgtgccgcc gcatgaagcg cgtcagattg ctgcgctcga tggtgtccgg
     4021 cgtcggccgc cagacgatct ctccgccgaa ggtgaatttg tcggtcatca tgcgggttat
     4081 tgtgccttaa ttgggatgaa atgggtagcg ggccaatgag gcgcaaaaac ctgcccttca
     4141 gcgaactgtc gatgggagac ggagcaggga atcaaactca tcggccgcgt taaccatcag
     4201 gccgcgcgga tttgtcagtc tgaagaaatc ccattgatca tcatgggaaa acgcgtccag
     4261 ataccagcat aacgcggcga tttgcggctc ccggttcacc acgtcgaggg cggtcgtcaa
     4321 ccagcccggc gggtagtttt gcgccggttc cgcggcatca tcgcgctgaa aggtgttggt
     4381 cgatgtgata tagacgggca atcctctgct gtcgggatgg gcgttgatca catccagcca
     4441 gtcctgatag acgcgaaatc cggcttgcgc gccggtggca ggtggttgcc gcagatcaat
     4501 gcgcggctcg tcggcccgtt cggtgcgcaa gccacgctcc agcgcctctg gatagaagcc
     4561 cccttctcgc acttgaggga tgtcgaacgt caccgcgcca acccgcgtgg tcactgtttt
     4621 cggcttgtag ccgttggcat ggccccggcg tgtatcagcc cgctcataga ccgccgcacc
     4681 taagtaattt atcgtaagtt ctttaacaag tgtgataaac tccgggcatg ccaaaagcca
     4741 gagattacca cctgaatgaa caggaattca aggcgattga agccgccatt cgtagtgaca
     4801 aacggccgga agttcgccaa agatgtacaa ccatgcgtct tctccacttc gggcataagc
     4861 ccaaagacgt ggctcaaatg caagccgtca gcattcccac catatacggt tggtacaatc
     4921 gctggcggca agatggcatt gaaggcttgg cgaccaagcc gcgcagcggc cggccatcga
     4981 agaccggaga agaatacctg aaggccgtcg aagaagcgct tgcgaaagag ccccgggcat
     5041 tcgggtatga cttttccctg tggacgatcg aacgcttgcg gctacacctg gaaaaggaaa
     5101 cgggaacagc cttgagcgat ggtcgcttgt gcatcctgct caagcgccac gggtaccgat
     5161 atcgtcgccc gaagtatgac ctgggccatt tacaggacaa agatgccaaa gcccaagcgg
     5221 ctgaattgtt ggacgagctc aaaaaaaggc cgcaaccgac gatttcgaac tcatctttgt
     5281 ggacgaaaca acgctgagcc tggatccgcc cctgcgagct tgctggatga agataggcca
     5341 acagaagcga gtcccggcga cccaaatagg cagtaagcga cgttggcaca tcctgggtgg
     5401 ctacaactgg ttgaaagata cggttgcctg gagcacggct gttacgaaga acacggcgac
     5461 ctttatcgag ttcctggaag aactgttgct ccggcagtat ccgagcgaac gcatcgtgtt
     5521 ggtgatggac aacgtttctt accacaagag tgccgctgct ttggcagcgc tgagtttgtt
     5581 tgaacatcgt gtggccgtta tctggctacc gccgtattgt tcagacctca gccccatcga
     5641 gcgtttctgg cgcttcttga aagacttggc ctgcgccaac catttggaag acaaaatcga
     5701 agatgtggtc ggtatggctg agaaggtgat gtctcatcag aatgacccca cttacgacta
     5761 ccgttttcat gtttctaaag acttacgata aattacttag gtactgttcc ctctcggcct
     5821 gcatcgccgt gttgatgacg atgcggatca gttccggcaa gatgcccagc ccttgttcgg
     5881 cgatctgttt cagcaatgaa tctggtaagg tacaatcctt ctggtaggtc atgatgtcgc
     5941 tacttggttg tttggcgata accgaagggt acatcgtggc ctgtctcgcg ccaccaattt
     6001 acagaaggaa agttacactt tcaagggagg agggatgaac ttgaagaagg acttgaatat
     6061 taggcgattg ttcctgcttg cttgtatggt gctgctatta tctgcgtgtg tagcaccggg
     6121 ttccgatata acgtctcaag ccggaagtcc tcaacctgct gtgctgaccg ataaggtgga
     6181 tgaaccggaa tccattgttg aagtggtatt cacgcccact cctttatcaa cgcctacaga
     6241 cgttccgact cagactccaa ccccagagcc agagccaacc caaactccaa ccccggaagc
     6301 aactccaatt cccccagaag ttccttttag ggtcgaggat ggaattgtgc agaaatggac
     6361 tgtcaatccg gttaccaatc aagaagagtg ggtgccagac gagagtttcc cagtacttga
     6421 gcctgaaccg ggtagacgta cccccgtgat cgaaagcatc gggtcagtta cgtatcaaat
     6481 aaatggggaa gaagttgggt actttagtgc gattagcgct atatatggta agacagaagc
     6541 tatatttata aatggagagt ggacgctcgc ctccgagatt aatgttaaag attttattgc
     6601 accagttgcc ccgggcgagg tggaatttgc taatgagaat tggagcgatg ggcgcaaact
     6661 aattgaatcc ggatctgtgg aatttttaga aaaattccaa caggcattga tcgatggcaa
     6721 tccagcttac tttagatggt atgtgggtag tgccgatatc tttttgaaca atgttttagc
     6781 agttgcgtca accagaaact atatgcttcc agttccccct gatggcgtac caaagctgct
     6841 gctgccatct tgggataaga cacgctatag cactgaattt gcgataagaa cgcgcaaccc
     6901 ccacaccgaa attaatactg atatcaattt tggaggtctt acggtattaa tcttcacccc
     6961 agaccaaatt aaaaatgatc ccgtccttaa agtaatcgtt aatggattgc ccatgaatta
     7021 cctcctttca gtccaatcat cggagagagg tcagtacgga attgtggttt tacccgatgg
     7081 atctctgttg tgggtacacg gtgaacagat gggccagcca aatcagccca aagacttcaa
     7141 aactaccctt cttggagagc gcccagaaaa tttttctggg attactcgcg cctttgccgc
     7201 cgcttatctt gaagctatga cgagagcgga aggtgagtca gtccgagtgt ttccactcaa
     7261 cccattcgat tatggagacc ccgaaatgat atctggtgat ggagttggtt tgcagccatt
     7321 cgtagaataa gcgtggattc caagttcttt ttgttttgat ggtcaacgga gaaccacatg
     7381 accctccctg ttgcacaata gcaaaaccat agccggcgta gctttcgacc tgggcttgct
     7441 tcccaacacg ctgcatcgat ggcgcgacga attgcggata gccggcgatg aagcctttcc
     7501 cggccggggg cagatgaagg cgagcgatga ggaagtgcga cgactggaac gggagataac
     7561 gcggctgcgg caagagcggg atattttata aaaagctacc acgttcttcg ccagggaaac
     7621 ccatgaggta ccgattcatc gatacgcagc gcgg
//
LOCUS       DAOMQG010000142         7645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4128_length_7645_cov_10.856785, whole genome shotgun sequence.
ACCESSION   DAOMQG010000142 DAOMQG010000000
VERSION     DAOMQG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4128_length_7645_cov_10.856785"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1810
                     /gene="glnA"
                     /locus_tag="PLC06_09140"
     CDS             407..1810
                     /gene="glnA"
                     /locus_tag="PLC06_09140"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRO90648.1"
                     /translation="MKPADVTKLIKDKHIEVIDAKFTDLFGQWQHFSLPVEHFSEESA
                     FEEGLGFDGSSIRGFQSIESSDMVLVLDPSTAIVDPICEAPTLSLVGDVYNPVTREPY
                     SRDPRHIARKSLKYLEGTGIADTAYFGPEAEFFVFDKVMYSAGEYSSAYQVDSIEGPW
                     NTGIFGYGHSVPHKRGYFPVAPVDTLQDLRSQMVRTLIESGVDVELHHHEVGTAGQCE
                     IDMRFDTMLKMADNVQLYKYIVKNVAQKHGKTVTFMPKPIYADNGSGMHTHQSLWKDG
                     KPLFYDEKGYAGLSQMALWYIGGILKHINALLAICAPTTNSYRRLVPGYEAPVVIAYS
                     AGNRSAAVRIPMYSKSPKAKRIEFRCPDPAANPYLAFAAMLMAGLDGVKNRIDPGDPA
                     EKDLFHDENMSNVTMTVGKLNEALDALEADHDFLLEGGVFTKDVLDAFIAYKYETEIG
                     PVSLRPHPYEFLLYYGV"
     gene            complement(1887..3080)
                     /locus_tag="PLC06_09145"
     CDS             complement(1887..3080)
                     /locus_tag="PLC06_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRO90649.1"
                     /translation="MERKWLGVILAAFLILCGAFLVVFIRQRADNAGGPVVAAAVEQD
                     VSPTATAPSPPATSTRRPQPAVIRVTRPPISPIAKSSGPVSTEVAPEPSPTSAPTETP
                     TTEPSPSPTETPTPTPVPTDPPTPTVEAVGTDWLRYLNLFRQQANLPPLLENTLWSLD
                     DAQHSRYMTYTGNLSHNEDAASQYYTKGGQAAAENGNIAAGFINSDPFKWAINYWMSA
                     PFHALPIIDPQLATTGFAEYRDVSGVQPLTATLDVKRGLGPLPDGVSYPIMFPRDGGL
                     TWVLRYSLPEFPEALSTCAGYQQPTGAPIMLQIGDGSQVPNVTASALFRDGEPLAHCR
                     FDETNFTHPNEFRQRTARLILDNRDAIIIIPQQPMLPGSTYSVRVDANGQTYTWSFRT
                     AAGPP"
     gene            3236..4855
                     /locus_tag="PLC06_09150"
     CDS             3236..4855
                     /locus_tag="PLC06_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRO90650.1"
                     /translation="MAADVPVNPLHAHPFASLREIARSLSAAWDLDTTLDLIARKTTE
                     VMRVNSCTIYLLDTDSQTLRLRASTGLARQALGRATLQIGEGMTGHAVARNRPIYAAD
                     AQHDPHFKWVDDTEEVRFRSLLAMPLVLNDRPIGALNVQTAEPRDYDEDEIELLALIC
                     DLAAGALAKAQLYDRQRRQLDDLRTLAQVSEAITSPQYLDDILTVVTGLAAGTVGAIA
                     CSIYLLESAGDDLDQTAKSPVFTPPAGTTQSDIATSDYAVLEEVAHTGRPHRAFRQDD
                     VVSYPLLAVPLSIQERTIGVLVCLGPVGHEFSESQEVLLTTLANQTALAIENARLVTS
                     AAVVREMHHRIKNNLQTVAMLMQLQLPEADRLETRQVLLTNIHRIRSIAAVHEILSEQ
                     GFRLVNVKRVLERIAQTTMTGMTRPNQAVEIAVRGDNLQLPSRTATALTVVVNELLQN
                     SLEHAFVGRASGRIDISLARTPDALMIIVRDNGIGLPDTIAPNLGLEIAEALVAGDLH
                     GEMQFNRPEAGTEITIRLPRGAERPDAAANE"
     gene            4891..5445
                     /locus_tag="PLC06_09155"
     CDS             4891..5445
                     /locus_tag="PLC06_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO90651.1"
                     /translation="MLILIADDESIIRLGIKSMLEGLGHQVLAAANGREAIRMAERQQ
                     PDLAILDIQMPYTDGLQAARTIMATRPLPILILTAFSEQDLIDKATDLPIQGYLVKPI
                     EPDALGAAIKVAVKRFAESQALATRTAQLEADLATRKLIDRAKGKLMKSGLSEEEAHQ
                     TIQDRARQSRQTMAAVAAMILQEH"
     gene            5485..6138
                     /locus_tag="PLC06_09160"
     CDS             5485..6138
                     /locus_tag="PLC06_09160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRO90652.1"
                     /translation="MTTRIYLIRHGGTAASTEDRFSGNTDIDLSDDGRRQAATLAKRL
                     TDDDIAAIYSSPMRRTIDTARIVGEPHHLTPIIRESLKEIHHGHWETMLREDVERLYP
                     EEYAAWVEDPFTFAPLGGKPGVMVMAEALPTIREIVANHPGQSVAVVSHKATIRLILC
                     GLLGIDGRRYRTLLDQSPASLNILDFKNPARARLILFNDISHYVNAISDIINPPDSI"
     gene            complement(6260..7057)
                     /locus_tag="PLC06_09165"
     CDS             complement(6260..7057)
                     /locus_tag="PLC06_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRO90653.1"
                     /translation="MIDLQPAIDYVQRQGSPQERAQLRHILHGEPPPRDVVEQFERSQ
                     RGDGGWAPFWAPDYGSLDATCYQLAQLKQLGVDMRSSMVIDAVRFLAERQAYDGSWAE
                     EPSVADAAPVWARSGDLAANLYVTANCAYWTAMTGLVPSAAHDAATWLSFHLDENGGL
                     PSFPHTHWLAAAVWSHENMDDETSKGLAYLRAGVPELSAGNLAWMVLALRQGGIPSAD
                     ETVASAVERLVELRDPAGPWPSDEGADNAAHVTVEAIMALKLCGRLS"
     gene            complement(7057..>7645)
                     /locus_tag="PLC06_09170"
     CDS             complement(7057..>7645)
                     /locus_tag="PLC06_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO90654.1"
                     /translation="MTLRYLIAYLVYNDGIQTIILVAATFAVDELGVPIQTILILVLM
                     IQFVAFGGALLFGRIARRIGAKKAIMISLVIWSGIVIYAYLFLNNQTQLFAMGAVLAL
                     VLGGSQALSRSLFSQMIPPEQEAEYFGFYEISERGTAWLGTAAFALAVQLTGSQRIAI
                     VSLIIFFVVGLLLLSRVDVRRAITEAGNDPGGVVL"
BASE COUNT         1677 a         2300 c         2259 g         1409 t
ORIGIN      
        1 ccggcccggt catcgaagtg gccgcccaga ctttggtcgc aatacgaact attaacgtac
       61 gacggacatc acaaggatgt ctactttcat caagtgtagc gatttgttta tcgcctgaat
      121 tcatgcactt tcaacaacat tcgagtcgtt gcaattatta atattcgtgc attgacacac
      181 agcgtcattt gagtatcatt gatacttgtg ttcacaggca ggtgccctga agggtagtgc
      241 caggtgaaac gaacagaata ttcaaaacag gccacgacgc ctcacgtacg acacgacggt
      301 tgtacggcga ggcgtttttc atttgagatc gcgagcctta cccggctctt cgcctgtcac
      361 aagaaatcaa tcggatatta tgcaaacttg tttgggagaa aagagaatga aacccgcaga
      421 tgtcacgaag ttgatcaagg acaaacatat cgaggttatc gacgcgaaat tcaccgacct
      481 gttcggacag tggcagcact tctcgctgcc ggtggaacac ttcagcgaag aaagcgcgtt
      541 cgaggaaggg cttggcttcg acggctcctc cattcgcggc ttccagagca tcgagtcaag
      601 cgatatggtt ctcgtcctgg acccctccac ggccatcgtc gatccgatct gcgaagcgcc
      661 gacactgagt ctggtgggtg acgtctataa tcccgtcact cgcgagccgt atagccgcga
      721 tccgcgccat atagctcgca aatcgctgaa gtatcttgag ggcaccggca tcgccgacac
      781 agcctacttc ggccccgagg cggagttctt cgtattcgac aaggtgatgt attcggccgg
      841 tgaatactcg tcggcctatc aggtggacag cattgaaggt ccgtggaaca cgggtatctt
      901 cggctacggt cactcggtgc cccacaagcg cggttatttc ccggttgcgc cggtggatac
      961 gttgcaggac ctgcgctcgc agatggtgcg cacgctaatt gaatccggtg tggatgtcga
     1021 gctgcaccac cacgaagtcg gcacggccgg tcaatgcgaa atcgacatgc gcttcgacac
     1081 gatgctgaag atggccgaca atgtccagct ctacaagtac atcgtcaaga acgtggccca
     1141 gaagcacggc aagacggtca cattcatgcc caagcccatc tacgccgata acgggtcagg
     1201 catgcacacc caccaaagcc tttggaagga cggcaagccg ctcttctatg acgaaaaggg
     1261 gtacgccgga ctaagccaga tggccctgtg gtacatcggc ggcatcctga agcacatcaa
     1321 cgccttgctg gccatctgcg cgccgacgac caactcctac cgccgcctcg tgccgggcta
     1381 cgaggctccc gtcgtcatcg cctactcggc cggcaatcgt tcggcggccg ttcgcatccc
     1441 catgtactcc aaatcgccca aggccaagcg catcgagttc cgctgccccg acccggcggc
     1501 caatccctac ctggccttcg ccgccatgct catggccggg ctggacggcg tcaagaaccg
     1561 gatcgacccc ggcgatccgg ccgagaagga cctgttccac gacgagaaca tgagtaatgt
     1621 gacgatgacc gtcggcaaac tgaacgaggc cctcgacgcg ctggaggccg accacgactt
     1681 cctgctggaa ggtggtgtgt tcacgaaaga cgtgctggac gccttcatcg cctacaagta
     1741 tgagacggag atcggcccgg tcagcctgcg gccgcatccg tatgagttct tgctctacta
     1801 cggtgtgtaa tttctgctaa agcagatcaa gactcaagtc ctgagagttg aaaggggcgg
     1861 gttaatgaca cccgcccctt tttgatttac ggtggcccgg cggccgtgcg gaagctccag
     1921 gtgtaggtct gcccattggc gtccacccgc acggaatacg tcgagccagg cagcatcggc
     1981 tgctggggaa tgataatgat ggcgtcgcga ttatcaagga tcaggcgagc agtccgctgc
     2041 cggaactcgt tggggtgggt gaagttcgtc tcatcgaaac ggcaatgggc cagcggttcg
     2101 ccgtcgcgga acaaagccga agccgtcacg ttcggcacct ggctgccgtc gccgatttgc
     2161 agcatgatgg gcgcgccggt cggctgctga tagcctgcgc aggtgctcag cgcttcaggg
     2221 aattcgggca gtgaatagcg cagcacccag gttaggccgc catcacgggg gaacataatg
     2281 gggtaactaa cgccatcagg cagagggccc aggccgcgtt taacgtccag cgtggccgtt
     2341 aacggctgaa cgcccgagac atcgcgatat tcggcgaagc cggtcgtggc cagttgggga
     2401 tcgatgatgg ggagcgcgtg aaacggagcc gacatccagt agttgatcgc ccatttgaat
     2461 ggatctgagt tgataaagcc cgcggcgatg ttgccgtttt cggccgcggc ctggccgcct
     2521 tttgtgtaat actggctggc ggcatcctcg ttgtggctca gattgccggt ataggtcatg
     2581 tagcggctgt gctgcgcatc atcaagggac cagagggtgt tctccagcaa aggcggcaga
     2641 ttggcctgct gccgaaacag attgagatac cgcagccagt ctgtccccac ggcttcgacg
     2701 gtaggtgtcg gtgggtcggt cggaacaggg gttggggtcg gcgtctccgt tggagagggc
     2761 gacggttctg tggttggcgt ctctgtcggc gcagaagtgg gcgatggctc aggggcaact
     2821 tcggttgaaa caggcccgga tgatttcgca atcggagata tcggcggacg cgtcacgcgg
     2881 atgaccgcgg gttgaggacg gcgagtggat gttgccggag gggagggcgc cgttgccgtg
     2941 ggggacacgt cctgttcgac ggccgccgca acgacaggcc caccggcatt atccgcccgc
     3001 tgccgaatga acaccaccag gaaagcccca cacaatatga ggaatgccgc cagaatgacc
     3061 cccagccact tcctttccat ttcggtactc ctttttaact cggatcgatg aaccggcgcg
     3121 aagccggtat ttctgcattg tcagaactac attgtaaaag gttataggaa aaaacgcatt
     3181 aggtattatt acacctgtta gcgagtcaga tcgggcgggc gtataattct cgccgatggc
     3241 agccgatgtt cccgttaacc cgctccacgc gcacccgttc gcctcgttgc gagagatcgc
     3301 gcggtcgctg agcgcggcgt gggatttgga caccacgctc gatctgatcg cccgcaagac
     3361 gaccgaggta atgcgcgtca actcctgcac tatttatctg ctggataccg acagccagac
     3421 attgcgactg cgggcgtcga ccggccttgc gcgacaggcg ctcggccgcg ccaccttgca
     3481 gatcggcgaa ggcatgaccg ggcacgccgt ggcccgcaat cggcccatct atgccgccga
     3541 cgcccagcac gacccccatt tcaaatgggt cgatgacacc gaagaagtta ggtttcgctc
     3601 cctgctggct atgccgctgg tgctcaacga tcgtcctatc ggcgcattga acgtgcagac
     3661 ggccgagccg cgagactatg acgaggatga gattgagctg ctggcgctta tctgtgatct
     3721 ggccgcggga gcattagcca aggctcaact ctatgatcga cagaggcggc aactcgacga
     3781 cttgcgcacg ctggcccagg tcagcgaagc tatcacctcg ccgcaatatc tggacgacat
     3841 cctcaccgtc gtcacgggcc tggccgccgg aaccgtgggc gcgatcgcct gttcgatcta
     3901 cctgctggaa agcgcgggcg atgacctcga ccaaactgcc aagtcacctg tgttcacccc
     3961 accggccgga acaacacaga gcgacatcgc aacgtcggat tatgccgtgc tggaggaggt
     4021 cgcacatacc gggcggccgc atcgtgcctt ccggcaggat gacgtcgtgt cgtatccact
     4081 attggccgtg ccgctcagca ttcaggaacg aaccatcggc gtgctggttt gcctgggccc
     4141 ggtcggacat gagttcagcg aaagccagga ggtgctgctc acaacgctgg ccaaccagac
     4201 ggcgctggct atcgagaacg cccgactggt gaccagcgcg gccgtcgtcc gtgagatgca
     4261 ccatcgcatc aagaacaacc tgcaaaccgt ggcgatgttg atgcaacttc agttgccgga
     4321 agccgaccgg ctggaaaccc ggcaggtgct tctgaccaat atccaccgca ttcgcagcat
     4381 cgccgccgtc cacgagatcc tgtcggagca aggcttccgg ctggtgaacg tcaagcgagt
     4441 gctggagcgg atcgcccaga ccacgatgac cggcatgacg cggccgaatc aggcagtgga
     4501 gatcgccgta cgtggggata atctgcaact gcccagccgg acggcgacgg ccctgaccgt
     4561 cgtcgtcaat gaactgctgc aaaacagtct ggagcacgcc ttcgtcggcc gcgcgtcggg
     4621 acgcatcgac atctccctgg cccgcacgcc ggacgcgctc atgatcatcg tgcgcgacaa
     4681 cggtatcggc ctgcccgaca ctatcgcgcc aaacctgggc ctggagatcg ccgaagcgct
     4741 ggtcgccgga gacctgcacg gtgaaatgca attcaaccgg ccggaggcag gcacggagat
     4801 caccatccgc ctgccgcgcg gcgctgagcg gcccgatgcg gcggcaaacg agtaggcagt
     4861 cctcatgggg cacatgacgc ggagcgaacg atgctgattc tgatcgccga tgatgaatct
     4921 ataatccgcc tgggcatcaa gtcgatgctg gaagggttgg ggcatcaggt gctcgccgcc
     4981 gccaacggtc gcgaggcgat tcgcatggcc gaacggcagc agcccgacct ggcgatcctg
     5041 gacatccaga tgccctacac cgacggcctg caggcagcaa ggacgatcat ggccacgcgg
     5101 ccgttgccca ttctgatcct gaccgccttc agcgagcagg acctaatcga caaggcgacc
     5161 gacctgccga tccagggcta cctggtcaag cctatcgagc cggatgcctt gggcgcagcc
     5221 atcaaggtag ccgtcaagcg cttcgccgaa agccaggcat tagccacgcg aacggcccag
     5281 ctggaagccg acctggcaac gcgcaagctc atcgaccggg ccaagggaaa attgatgaaa
     5341 tcaggtcttt ccgaagagga agcccatcaa acaattcagg atcgcgctcg gcagagccgg
     5401 caaacaatgg ccgccgtagc cgccatgatc cttcaggagc attagaagac gatggccgaa
     5461 agcggaagga gacaagttta agagatgacc acgcggatct atttaatccg gcatggcgga
     5521 accgcggcca gcactgagga caggttttcc ggtaacaccg atatcgatct ttccgatgac
     5581 ggacgccggc aagctgctac cctggctaaa cgactgaccg atgacgacat cgccgccatc
     5641 tattcaagcc caatgcgacg aactatcgat acggcgagga tcgtcggcga accccaccat
     5701 ctgaccccca tcatcaggga aagtttgaag gaaatccatc acgggcattg ggaaacgatg
     5761 ttacgcgagg atgtggaaag gctttacccg gaagaatacg cggcctgggt ggaagacccc
     5821 ttcacctttg cgcctttggg cgggaaaccg ggcgtgatgg tgatggccga ggcgttgccg
     5881 accattcggg aaatcgtagc taaccatccc ggccaatccg tagcagtcgt gtcccataaa
     5941 gcaaccatcc ggctgatcct ctgtggactg ctgggcattg acggccgaag atatcgcacc
     6001 ctgttggatc aatcgccggc gagcctgaat attctcgatt tcaagaatcc ggcacgggcg
     6061 cgactgattc tgtttaacga tatttcgcat tatgtcaatg ctatctccga cataatcaat
     6121 ccaccggatt caatatgagc ctttatcttc cgggggtgag cccgggttcc tcatacctaa
     6181 tagccgccat ttggcaaaca gttccagcag gctctccgcc aacgcatagc tctcctgcat
     6241 aaaatgagat cgatcgcttt cacgacagac gcccacacag cttaagcgcc atgatggctt
     6301 caacggtgac gtgcgcagcg ttatcggcgc cctcatccga cggccatggc ccggccgggt
     6361 cgcgcagctc cacgagccgc tctacggccg aggccactgt ttcgtcggcc gaagggatgc
     6421 cgccctgccg caaagccagg accatccagg ccagattccc ggccgagagt tcgggcacac
     6481 cggcgcgcaa ataggccagt cccttgctcg tttcgtcgtc catattctcg tgactccaga
     6541 cggccgcagc cagccaatgc gtgtggggaa aggacggcaa tccaccattt tcatccaaat
     6601 ggaaactgag ccaggtcgcc gcgtcgtggg cggctgacgg gacaaggcct gtcatggctg
     6661 tccaataggc gcaattggcg gtcacgtaga ggttcgccgc aagatcgccc gacctggccc
     6721 acaccggcgc ggcatcggcc accgatggct cttccgccca cgaaccgtcg tacgcctgtc
     6781 gctcggccag aaagcgcacc gcgtcgatca ccatcgagga gcgcatatcc acgccaagct
     6841 gcttcagttg agccaactga taacaggtcg catccagcga gccataatcc ggcgcccaga
     6901 aaggggccca gccgccatca ccgcgctggg agcgctcgaa ctgctcaacc acgtcgcggg
     6961 gcggcggctc gccgtgcaga atgtggcgca actgtgcccg ctcctgaggt gaaccctgcc
     7021 gctggacgta atcaatggca ggctgaagat cgatcattac aaaaccacgc cacccggatc
     7081 attgccggct tcggtaatag cccgacgcac atcgacgcgg ctcaatagca gaaggcccac
     7141 gacaaagaag atgatcaggc tgacgatggc gatacgctgc gagccggtca attgcacggc
     7201 cagcgcaaac gccgcagtgc ccaaccacgc cgttccccgc tcgctgatct catagaagcc
     7261 aaagtactca gcctcttgct caggcgggat catctgcgag aagagggagc ggctgagcgc
     7321 ctgcgagccg ccaagcacga gggccagcac cgcgcccata gcaaagagct gagtctgatt
     7381 gttcaggaac aaataggcgt agatgacgat acccgaccag atgacgaggc tgatcatgat
     7441 cgccttcttg gcaccgatac gtcgggcgat tcggccgaac aggagcgccc cgccgaacgc
     7501 cacgaactgg atcatcagca caagaatgag gatcgtctgg atcgggacac ccaactcatc
     7561 gaccgcgaag gtcgccgcca cgaggatgat ggtctggatg ccatcgttgt agacgagata
     7621 agcgatcaaa taacgcagcg tcatc
//
LOCUS       DAOMQG010000143         7601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4164_length_7601_cov_10.014312, whole genome shotgun sequence.
ACCESSION   DAOMQG010000143 DAOMQG010000000
VERSION     DAOMQG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4164_length_7601_cov_10.014312"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PLC06_09175"
     CDS             <1..1242
                     /locus_tag="PLC06_09175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO90655.1"
                     /translation="ILFLSQLIPYPLDAGPKVRAYYVLRHLAEAGHEVTLLAFSRESD
                     SAASIDHLRQFCVAVHTVPMPRSRPRDAYHAARSLLTGQPFLIARDHVPEMIEKVKGL
                     AVSEAFDAVHADQLWMAPCALAAWEAVVPPRPRLVLDQHNAVYLIPQRLAESGRGALT
                     RWLLRREARLMRAYELDTLQKFDDVVWVTAEDRAAVALPEVGSPRQRVIPICVDPENT
                     PPIRRASRPHRVTFLGGLHWPPNAAGIVWFAREVWPSVRAAAPELTLTVIGKSPPGEL
                     LSAAAEPGSRIEVTGYVDDPREYLAETAVFVVPLHAGGGMRVKIVEAWLWGLPIVSTT
                     IGAEGILSSNGRDMLLADTTAAFVAAILRVVNEPGLADRLAESGRRQVEDHYDWSTEY
                     ASWDVIYGDAVLEQTAALVIG"
     gene            1242..2591
                     /locus_tag="PLC06_09180"
     CDS             1242..2591
                     /locus_tag="PLC06_09180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90656.1"
                     /translation="MQLLSGWTTRKILMMIIGVSVVARIAAALYLGNSVEVLPGTYDQ
                     MSYHELALRVLSGHGFSFGRPSWPLTAAGAPTAHWSFLYTFYLSAVYAIFGENPLAAR
                     LIQAVVVGVLQPYFAYRLGRHVFDERTGLLAAAFTAVYIYFIYYAATLMTEPFYITAV
                     LGALLGLLHLRRPAPDETRTALAVGAALGIAVLLRQLFLMLIPFLLLWLWSVRWWDAR
                     RRLAGTSDDLGLVRFHLPWRPTAIIVAFVVLLILPFTLYNYGRFDRFVLLNTNAGYAF
                     FWANHPIYGTQFQAILPPEMGSYGSLIPESLRDLDEPALEKALMERGMQFVIDDPIRY
                     MQLSLSRVPIYFMFWPSADSGTLSNISRVGSFGLFLPFMLAGLIFSFAHRETRQQAMA
                     DASLLLLFAVLYSAMHLLSWSLVRYRLPVDAVLLVYAGLAVSAIWSRLQRNASSASS"
     gene            2705..3925
                     /locus_tag="PLC06_09185"
     CDS             2705..3925
                     /locus_tag="PLC06_09185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO90657.1"
                     /translation="MRVLFLVPYMPNAIRTRPYNLIRSLARNGHEITVLTLTQDAAEV
                     ADADMLRVEGIRVVARPLSRLRSLANSTAALVGREPLQAAYCWQSSLATDLSALLQSE
                     IFDVVHVEHLRGARYGLRAKADLATLGLRTPVVWDSVDCISHLFRQAATHSTNRFRRY
                     ITRLELPRTERFEGELLNEFDQVLTSSPMDKEALQQLANGQYEVMTPIQVLPNGVDFN
                     RFRPDETVSSEPDTLILTGKMSYHANITMAQFLVRGIMPHIWAKKPEVRVMIVGRTPA
                     PEVRALAMDSRVTVTGSVPDIRLFLNQATVAVAPIVYNAGIQNKILEAMACGTPVVTT
                     PAAVAGLGARSGEHLLVAEGAEALAEAALVLLESQEKRQVLSAAALAYVRKNHDWSII
                     SEQLAVIYGSGKYP"
     gene            4115..5623
                     /locus_tag="PLC06_09190"
     CDS             4115..5623
                     /locus_tag="PLC06_09190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRO90658.1"
                     /translation="MGSSIHYLDNQSGGRLEGVRQPIASASPWVQWTLYRLSLIINDV
                     VLTLGAFMFAAWVRFNVPLSVFNLEARPTIPQLPIIIFGFVPLWLAIYASYGLYQKKS
                     LLGGTQEYAGVFQATSIGILVIVVVDFMQPLFAPARGWVLLAWIFTICFVVIGRFLLR
                     RAVYHLRRRGYFLSPALIIGKNAEALSLAEQLLLWRTSGLAVRGFITTGLEEDGRDVE
                     SELPVLGGFEHLDDLVERLNIGELILATSAMSQEQILLMFKRYGVSPSVNMRLSSGLF
                     EIITTGLELKEVASVPLLRINQARLTGMDWLLKSVLDVSLALIAIAVALPLMAVIALS
                     IRLDSPGTVIYRRRVMGLNGVQFDAYKFRTMYCNGDELLAAKPDMQSMLEQSCKLKSD
                     PRVTRVGQILRKHSLDELPQFFNVLRREMSVVGPRMIAPDELARYEQWDMNLLTVPPG
                     ITGLWQVSGRSDLSYGERVQFDMRYIRNWSIWLDFHILMRTVLVVINGNGAY"
     gene            5806..7518
                     /locus_tag="PLC06_09195"
     CDS             5806..7518
                     /locus_tag="PLC06_09195"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRO90659.1"
                     /translation="MELRTYLRPLLKWWWLILISTLLAGITSYFAVSQQPPVYQARAT
                     LLIGGAINNPNPTGNEFWLSQQLAQTYSDIAQRDVVRKAVMDELGLTWLPTYTAQSVG
                     STQLLEIRVNDSVPERAMVVANELANQLILRTPTSGGQQGDVERQAFIKRQLDELELK
                     IAETNSEIVARQNELAGLFSARQISDLQDQIRALEGKQDALQANYASMLASSTQGAAN
                     SLSVIEPATLPVDPIGPEVLMTVITAAAIGLSLGMAAAYLLEYLDDTIKTPEDIRRIA
                     DLPTLTGISDAKSVDTDSQRLVTLANPRSPIAEAYRSLRTAVLFANVDQAARSIVVTS
                     AGPQEGKSFTSANLAVVLAQSSYRVLLIDADLRRPTQHKLFNKQAAYGLTNLLLDMPP
                     SFDSSRLGDMYSQLSRAIMTTSIQGLFLMTTGDLPPNPAELVGSVRMKSLIKALTTRF
                     DFIIIDTPPVLAVTDAVVLGTRVDSVLLVTRSGYTRQSQIKHAVLMLREVNAKISGVV
                     LNRINKQSGEYYYHHYNSYYSTANDKEDKPKDDPGMPVTEDQPRGVARQHEPARGFLT
                     RLLS"
BASE COUNT         1510 a         2020 c         2190 g         1881 t
ORIGIN      
        1 atcctcttcc tctcccaact catcccctac ccgcttgatg ccgggccgaa ggtgcgcgcc
       61 tactacgtcc tgcgtcatct ggctgaggcc ggacatgaag ttaccctgct ggccttcagt
      121 cgggagagcg actcggccgc gagcatcgac catttacgcc aattttgtgt cgccgtccac
      181 accgtgccga tgcctcgttc gcggccgcgc gatgcttacc acgccgcgcg gagcctgcta
      241 acgggacagc cgttcctgat cgcccgcgac cacgtcccgg aaatgatcga aaaagtcaaa
      301 gggttggcgg tctcggaggc cttcgacgcc gtccacgccg accagttgtg gatggccccc
      361 tgtgcgttgg ccgcatggga agcggtcgtt ccgccgcggc cgagactggt gctcgaccag
      421 cataacgccg tctatctcat cccccagcgg ctggccgaga gcggccgggg ggcgttgacc
      481 agatggctgc tccgccgtga ggcgcgtctg atgcgagcat atgagttgga cacgctgcaa
      541 aagtttgacg atgtggtgtg ggtgacagcc gaggatcgcg ccgccgtcgc tttgccggag
      601 gtgggcagtc cacggcagcg cgtcattccc atttgtgtcg atccggagaa tacgccgccc
      661 attcgccgag cgtcccggcc ccatcgcgtc acctttctgg gcggtctgca ctggccgccc
      721 aacgccgccg gcattgtctg gttcgcgcgc gaggtttggc ccagtgtgcg cgcggccgcg
      781 ccggaactga cgctgaccgt catcggcaaa tcaccacccg gcgaactgct ctcggccgcg
      841 gccgagccgg gcagccggat cgaggtgacg ggctatgtag atgacccacg agaatatctg
      901 gccgagacgg ccgtgtttgt cgtgccgctc catgccggcg gcgggatgcg ggtcaagatc
      961 gtcgaggcct ggctgtgggg attgccgatc gtctcgacga cgattggggc ggaagggatc
     1021 ctaagttcca acggccgtga tatgttgctg gccgacacga ccgcggcgtt tgtcgcggcc
     1081 atcctgcgcg tggtcaatga gccaggcctg gccgataggc ttgccgagtc cggtcgccga
     1141 caggtggaag atcactacga ttggtcgact gagtatgctt cctgggacgt tatttacggg
     1201 gacgctgttc ttgagcagac tgctgccttg gtaataggat aatgcaactg ttatcgggtt
     1261 ggaccacgcg aaaaatactg atgatgatca ttggggtctc cgttgtcgct cggatcgctg
     1321 ccgccctcta tctcggcaac tcagtcgagg tattgccggg gacctatgat cagatgtcct
     1381 atcacgagct ggccttgcgc gtgctgagcg gccacggctt ctcattcggc cgcccgtcgt
     1441 ggcccctgac ggccgccggc gcgcccaccg ctcattggag cttcctctac acattctatt
     1501 tgtccgcggt ttatgccatc ttcggcgaaa accctctcgc cgctcgactc atccaggcgg
     1561 tggttgtggg tgtactccag ccgtacttcg cctatcggct ggggcgacac gtctttgacg
     1621 agcgaacggg cctattggcc gcagccttca cggccgtcta catctacttc atctactacg
     1681 ccgccacgct gatgaccgag ccgttctata tcactgccgt ccttggtgcg cttctgggat
     1741 tgttgcattt gcggcggccc gcgcccgacg agacacggac ggcgctggcg gtgggcgcag
     1801 ctctggggat tgccgtcctg ttgcgtcaat tattcctgat gctgatccct tttctgctgc
     1861 tatggctatg gagcgtgcgt tggtgggatg ctcggcgaag gcttgctgga acttccgatg
     1921 atttgggcct ggttcgcttt catctcccct ggcgccccac agcgatcatc gtcgcgttcg
     1981 ttgtgctgtt gattctgccc tttacgcttt ataactacgg ccgcttcgac cgcttcgtat
     2041 tgctcaatac aaacgccggc tacgccttct tctgggccaa tcacccgatc tatggcacgc
     2101 aatttcaggc catcctgccg ccggaaatgg ggtcttacgg gagtttgatc cctgaatcct
     2161 tgcgcgatct cgatgagcct gcgctggaaa aggcgctgat ggagcgcggc atgcaattcg
     2221 tgatcgacga tccaattcgc tatatgcagc tctcgctaag tcgtgtgccg atctatttca
     2281 tgttctggcc ctcggccgat tcggggacgc tcagcaatat ttcgcgtgtc ggcagctttg
     2341 gcctgttcct gccgttcatg ctcgccggtt tgatcttctc ctttgcccat cgcgagacac
     2401 ggcagcaggc gatggccgat gcctcattgc tgctattgtt tgccgtgctt tattcggcca
     2461 tgcatctgct gtcgtggtcg ttggtgcgct atcgtttgcc ggtggatgcc gttcttttgg
     2521 tctatgcggg gctggctgtt tcggctatct ggtctcgcct gcaacgtaac gcatcgtcgg
     2581 caagctcatg acaaatcgta tttcggcgtt gggcttcagc cgattatagc cgggagccat
     2641 tcggcttaat ccgagcgatc tcgtcgccgt tattgttcgt aaatcaccac ccaacgacta
     2701 acacatgcgc gtccttttcc tcgtccccta tatgcccaat gccattcgca cgcgcccgta
     2761 taaccttatc cggtcgctgg cgagaaatgg gcatgaaatt accgtcttga cgctgacgca
     2821 ggatgctgcg gaggtagccg acgccgatat gctgcgagtt gagggtatac gggtggtcgc
     2881 gcggccgctg tcccggttgc gttccttggc gaatagcaca gcggcgttgg tggggcggga
     2941 accgctccag gcggcttatt gctggcaatc ctcgctggcg accgacttat ccgctctttt
     3001 gcagagtgag atatttgatg tggttcacgt cgagcatctg cgcggcgcgc gctatggtct
     3061 acgggcaaag gcggatctcg ccacactagg gttgagaact cccgtggtgt gggatagcgt
     3121 cgattgcatt agccatctct tccggcaggc cgcaacgcac agcaccaatc gtttcagacg
     3181 ttacatcacc cggttggaat tgccgcgtac agagcggttc gagggcgaat tactcaacga
     3241 gttcgaccag gtgctgacct cttcaccgat ggataaggaa gcattgcagc aattggcgaa
     3301 cggtcaatat gaagtgatga ctcccatcca ggtgctgcct aatggtgtgg acttcaacag
     3361 gtttcggcct gatgaaactg tgagcagcga acctgacacc cttatcctga ccggtaaaat
     3421 gagctatcac gccaatataa cgatggcgca atttctcgtc aggggaatca tgccccacat
     3481 ttgggccaag aaacccgaag taagggtgat gattgtcggc aggacccctg cgcccgaagt
     3541 acgtgccttg gcgatggatt cccgtgtgac agttaccggt agtgtcccgg atatccggct
     3601 atttttgaat caagccaccg tggctgtagc tccaatcgtc tacaacgcgg gcatccaaaa
     3661 caaaatattg gaggcgatgg cttgcggcac gccggtagtg acgacacctg cagccgtcgc
     3721 cgggttggga gcgcgatcgg gcgagcatct tttagtggct gaaggagccg aggctctggc
     3781 cgaagcggcg cttgttcttt tggagtcgca agagaaaagg caggtgttgt ctgcggctgc
     3841 cttggcttat gttcgaaaga accacgattg gtcgattatc agcgagcagt tagcggtaat
     3901 atatggctcg ggaaaatacc cgtgagcttg ctgattcctt gtcggtgttg aaacccgtaa
     3961 tcccgatcgt aaggtttcgt gagcaaaata gagttagagt tgttccttgc cggtgaacag
     4021 cgatcgatga acggtaaaga gtaagcagca tagagcgcga tcatggagta ttgtcatttc
     4081 aatgctgcct attgatcgga tcaggaggct ctctgtggga tcttcaatac attatctgga
     4141 caatcaatcg ggcggccgtt tggaaggggt tcggcagcct atcgcttcgg cctcgccatg
     4201 ggttcagtgg acgttatatc gcttgtcatt aatcatcaat gatgttgtcc tgacccttgg
     4261 tgccttcatg ttcgcggcct gggttcgatt caatgttccc ctatccgtct ttaacctgga
     4321 ggcacgccca acgattccgc aattgcctat catcatcttt ggatttgtgc ctctttggct
     4381 cgccatttat gcttcgtatg gcctgtatca gaaaaaatcc ttgttgggcg ggacgcagga
     4441 atacgcagga gtttttcagg cgacgagcat tggtatcttg gttatcgtgg ttgttgattt
     4501 tatgcagcca ttgtttgccc ctgcccgtgg ctgggtgtta ttggcgtgga tatttacgat
     4561 ctgttttgtg gttatcggtc gatttttgtt gcggcgcgcg gtgtatcatt tgcggcgacg
     4621 cggctacttc ctcagtccgg cgctgatcat cggaaaaaat gcggaagcct tatccttggc
     4681 tgagcagcta ctgctgtggc gcacgtcagg gctggcagtt cggggcttta tcacaacggg
     4741 ccttgaagaa gatgggagag acgttgaaag tgaattgccg gttctggggg gttttgaaca
     4801 cctggatgat ctcgttgaac gactaaacat cggtgagttg atcctggcta caagtgcgat
     4861 gtctcaggag cagattctct tgatgttcaa gcgatacggt gtctcgccca gtgtcaatat
     4921 gcggttatcg agtggattat tcgagatcat tacaacaggg ctcgaactca aggaggttgc
     4981 atccgttccc ttgctgcgta ttaatcaagc acggttgaca gggatggatt ggctgctgaa
     5041 atcagttctg gacgtttcat tagccctcat tgcgattgcc gtcgctctgc cgttaatggc
     5101 tgttatcgcc ctcagcatta gattagattc gccaggtact gtgatttatc gcaggcgtgt
     5161 tatgggatta aatggcgttc aattcgacgc ttacaagttc cgcactatgt attgcaacgg
     5221 cgatgaatta ctggccgcga agccggatat gcaatcgatg ttggagcaat catgtaagct
     5281 taagagcgat ccacgtgtta ctcgggtagg gcagatcttg cgcaaacata gccttgatga
     5341 actcccgcag ttctttaacg tacttcggcg cgaaatgtcg gtcgttggtc cgcggatgat
     5401 tgcgcctgac gaattggcgc ggtatgagca gtgggatatg aaccttttga ccgtgcctcc
     5461 aggcatcacc gggctatggc aggttagcgg ccgctccgat ctttcatatg gcgagcgtgt
     5521 gcaatttgac atgcgctata ttcgaaattg gagtatttgg ttggactttc atatcctcat
     5581 gcgcacagtc ctggtcgtta tcaatggtaa tggcgcatat tgacgcgtcg cgaatgagaa
     5641 tatggaagcg agccaaaagc ataggacaat agaattgacg gtttgtttat taacgcgatt
     5701 tatacggcct gacccggtga tcagccatat cattggttta agggcaattg tgagattaat
     5761 gtcctgtcgt tccggttgtt tagctgttca gatggataag agaaaatgga gttaagaaca
     5821 tatctcaggc cacttctgaa atggtggtgg ctcatcctaa tctccacttt gctcgccggc
     5881 ataacgagct attttgcggt gagccagcag ccgccggttt accaggctcg cgctacgttg
     5941 ctaattggtg gtgccattaa caaccctaac ccaaccggca acgaattctg gcttagccag
     6001 caattggctc agacctatag cgatattgcc cagcgggatg tggttcgcaa ggcggtgatg
     6061 gatgaactag gcctgacatg gctgcccacc tatacagccc aatctgtggg gagtacccaa
     6121 ctgctcgaga tacgggtgaa tgatagtgtg ccggaacgcg ctatggttgt agccaatgag
     6181 ttggccaacc aattgatttt gcgcactcct accagtggtg gccagcaagg ggatgtcgag
     6241 cgacaagctt ttattaagcg gcagcttgat gaactggaat taaagatagc ggaaacgaat
     6301 agcgagattg tcgccaggca aaatgaactg gcggggctat tcagtgcacg ccaaatttcc
     6361 gacttgcaag accaaatccg cgcgcttgag gggaagcaag atgcgctaca ggcgaattac
     6421 gcctctatgt tggctagctc cacacaggga gccgcaaata gtttgagcgt gattgagccg
     6481 gccacactac ccgtcgatcc gattgggccg gaagtattga tgacggtcat cacggccgcc
     6541 gctattggcc tttcactggg tatggcagct gcttacttgt tggaatacct ggacgataca
     6601 attaagaccc cggaagatat ccgccgtatt gcagatttac ccacgcttac cggtatatct
     6661 gatgcaaaga gtgtggatac ggactctcag cgtctcgtca cccttgccaa tccacgttca
     6721 ccgattgcgg aggcttatcg ttctttacgg acggccgttc tatttgctaa tgtcgaccag
     6781 gcagctcgct ccatcgtggt gaccagtgcc ggaccgcagg agggcaagag cttcacttcg
     6841 gccaacctgg cggttgttct tgcccaaagc agctaccggg ttttgctcat cgacgcagac
     6901 ctgcgccggc cgacccaaca taaactcttc aataagcaag ctgcttacgg gttgaccaat
     6961 ctgcttctgg acatgccgcc ttccttcgat tcgtcgcgtc tgggtgacat gtattcccag
     7021 ttgagtcgcg caattatgac gacatctatc cagggattat ttctgatgac cacgggtgat
     7081 ctgccgccca accctgccga gctggttggc tcagttcgca tgaagtcctt gattaaggcc
     7141 ttgacgacgc gtttcgattt catcatcatt gacacaccgc cggtattggc agtgactgac
     7201 gcggttgtgc ttggcacgcg tgtggatagt gtcttgctag tgacgcgttc cgggtacact
     7261 cggcagagcc aaataaagca tgctgtcctg atgttacgag aggtgaacgc caagatttcc
     7321 ggcgtagttt tgaatcgcat taataagcaa agcggggaat actattatca tcattataat
     7381 agctactact cgacagctaa tgataaggag gataagccga aagatgatcc ggggatgcct
     7441 gtgaccgagg atcaaccgag aggtgttgca cgacaacatg aacctgctcg tggtttcctg
     7501 accaggctcc tgagctaagt gactgacagg cctgcaccgg gtcgactacg ggagcggatg
     7561 ggagtcgaac ccacagccgc ctactatgcg tagccggcca c
//
LOCUS       DAOMQG010000144         7519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4223_length_7519_cov_5.937165, whole genome shotgun sequence.
ACCESSION   DAOMQG010000144 DAOMQG010000000
VERSION     DAOMQG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4223_length_7519_cov_5.937165"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..694
                     /locus_tag="PLC06_09200"
     CDS             92..694
                     /locus_tag="PLC06_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90660.1"
                     /translation="MAKNLKSTVSHTAKSAAPWRKGVPWWLVLIEGIVLAGVGLFMFF
                     GSSSTMVVIMGWIIALVLVAVGAISLYLVSQVTEQSPSRKFTMIHGLVAVGAGGLVIL
                     LRLLNMLLPDTAAVVLGFGCLVYGGMGLYTWINKGLVPLRRVSVIGAIFFLIIGALLL
                     LQAFGMATVGMVVQFITIASLIAGIALILWSFALKNSADA"
     gene            732..1316
                     /locus_tag="PLC06_09205"
     CDS             732..1316
                     /locus_tag="PLC06_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90661.1"
                     /translation="MENLLWGIIVLVCGVFIASYGNVLFRFALAFMGFALGFSLVMWL
                     GGGLDSGLRIIFGIVVGGVLALVFYMMVKFMLHMAGGVVGLVIMLALLGMFRLGGLNV
                     GAFGWVLAAAAAVGGGFIGNRLGNKVVVLASALAGSYLVVLGLGALYKVGVDSTQPFE
                     TLSAAFPLVLFLAIFAISFLAQNQAQNLRLRFLR"
     gene            complement(1421..5335)
                     /locus_tag="PLC06_09210"
     CDS             complement(1421..5335)
                     /locus_tag="PLC06_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO90662.1"
                     /translation="MHTRYVRLLTALVGLVVLSGLSAWVSKEAKAQSFPIYLPMTIGG
                     NGNQQVTIPETTEVITGQTLAQLESVSADGEQFVFDGSPAALDDLDIGDVMVGAPTAA
                     APDGFLRKVTGLQDTGNKRIVTTTPATLDEAITDGSLDLSLTLSPDKVASTILMPGVS
                     MAPSDRSGMAADTFSYTLDEFVIYDRDGNDATKNDQFTADGTVAVSQDIDFGFDIRGG
                     SLEKMDFIITSDTNSELRYKASVGLGATLVEKKKVLARHTFQPITVMVGFVPIVFTPE
                     LAIVLGVDGSVEAETTFGVQYSTHMEFGLRLENGNWGMVGDASDDFSPLPVTVDKQFK
                     IQAGVGPQLAIKLYGVVGPYAEAKIFGALALDSLLPFKWKLYAGLTVPVGVKLEVFSK
                     LFVDISRTVIEIKILIAEGGDTSNDPPFIPANPDPPHLATGRPLGLRLAWTGGDPDGD
                     PTSYDVFMEFGDSSPDVMVCNTAHVAYCDVTGLDPNETYYWRVVAEDNQGASTSGPAW
                     RFDTAGGVNQPPFTPSSPIPANNSDQQEIYLQLKWVGGDPDGDPTTYTVFLEAGDNTP
                     DAAACSAISVLACDVGPLSENTTYYWRVEATDSAQNKATGPVWKFTTRQGGEPIKTID
                     VAVIIDSSGSMAWNDPANMRKTAAKVFIGAMVSGDMLTIVDFDGGVRVPFHLQTLTND
                     RSAPLAAVDTIDSNGTTNIGLGLQTGYDQLVSSPNANPKAAVLLTDGDGEYFNQAQLY
                     RDKGWPVFTIGLSGDANEALLRQIASETGGQYFSLTNPNQLSQVYFAIQAAVTGSDVV
                     VETAFVMEQGQSATIPAAIDTGQNTANFVVNWPGSRVDATLVDPNGRVITPDTAQNDA
                     TIYHAKGATYEIYRIANPPAGQWQIELYGAELDPGGEEVTVQVSQRDNELVSGAWGEL
                     GSGSASGGGISQTADDSSRPAITHVGDDIYAAWVEDRAKQSDIYVRRWNGNAWVEVGA
                     GSASGGGVSNTATISDSPWLEAAPDGSIYLAWAEKTAGGYEVYVRRWDGSTWGEIGAG
                     SASGGGISQTATESEWPSMGISTSGVVYVAWEESQAADKEIYVRHWNGNAWAEVGVGS
                     ASGGGISNNAGDSGRPFLAVDQNGNPIVGWSDDTPGDTEVYARRWSGGTWGEVGGSAS
                     GGGISNNAGDSRPPVIGVDSTGIIYVTWPDNTGGHFDIYVKSWDGNAWVEVGAGSAAS
                     GGISGSSEVSLAPHVAVAPDDTVYVIWYERLGANTDILIRRWNGSFWVEVGAGSATGG
                     GISNTLGSSSLPSVTITGANMPVVAWNDNTSGNYEIYVKAWR"
     gene            complement(5483..6322)
                     /locus_tag="PLC06_09215"
     CDS             complement(5483..6322)
                     /locus_tag="PLC06_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRO90663.1"
                     /translation="MTIFRAVPDHVWAFDLEWVPDSASGRRACNLPAATPDDEVYAAM
                     WADAGATAEEPRPYLKTMLCRVVSLAVVMRKVQGGGVSLTLFSLPQSEAMPERELIGR
                     FLKGIGDATPRPQLVGFNSRESDLPILIQRGMAQGVSAPGFAHRPEKRWEGFDYFDKY
                     NEGHIDLKEVVGGWGKATPSLHQFATACGIPGKIDTSGDNVIDLWLAGDVRRIVEYNE
                     FDALTTYLLWLRAAHFAGHFNDAGHAEEVERVRELIGKRIAAGQTHLARYLARWDELG
                     GIV"
     gene            6701..>7519
                     /locus_tag="PLC06_09220"
     CDS             6701..>7519
                     /locus_tag="PLC06_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90664.1"
                     /translation="MNVPAMPRWVLLIVVLLLLAAARPFGRLLAQSSTLYGTLEIVWG
                     DPQTGGAAVTHYYLTTDDGRTVRLVLPETMTVDLLALNGRRVIVESRGPTRNGTGSGQ
                     PELQVDAIRPADPSPIAPAPLVEGGQPNVTLLCSFPDVAATHKPLSYYQEMYRDTAPG
                     LNHYWQDLSYGLANVAGSAAFGPYAMPNPLAFYMNNGEADLTQLATDCVAAADATVDF
                     PQFEGINMAFNANIGCCAWGGKRTMTLDGVTKTYATIWLPPWASNTISVVMHEMG"
BASE COUNT         1350 a         2389 c         2244 g         1536 t
ORIGIN      
        1 tcccttatgt tgacccggcg atattaatga tatacttata ttgtcataat tcgtcttgtc
       61 cttaatccgg ttcgaggagc ggaattctgc catggcaaag aatctcaaat cgactgtctc
      121 ccacaccgcg aagagtgccg ccccatggcg caagggggtc ccctggtggc tggtgctcat
      181 cgagggtatc gtgctggccg gcgtgggcct ctttatgttc ttcggcagct cgtcgacgat
      241 ggtcgtcatc atgggctgga tcattgctct ggtgctcgtc gccgtgggcg cgatcagcct
      301 ctatctcgtg tcgcaggtca cggagcaaag cccgtcgcgg aaattcacga tgatccacgg
      361 cctggtggcc gtcggcgcgg gcgggctggt catcctgctg cggctgctga acatgttgtt
      421 acccgatacg gcggccgtcg tgctgggctt cggttgtctc gtctacggcg gcatggggct
      481 gtacacgtgg atcaacaagg gcctcgtccc gctgcggcgc gtgtcggtaa tcggcgcgat
      541 cttcttcctc atcatcggcg cgctgcttct gctgcaagcc ttcggcatgg cgaccgtcgg
      601 catggtcgtc caattcatca ccatcgccag cctgattgcg ggcatcgcgc tcatcctctg
      661 gtcgtttgcc ctgaaaaaca gcgccgacgc ctgacgcgct aaatcgtctt tgatatcaac
      721 aggagaaaag aatggaaaac ttgctgtggg gaatcatcgt tctggtatgc ggggtcttca
      781 tcgcctcata tggcaatgtg ctgtttcgct tcgcgctggc cttcatgggc tttgcgctcg
      841 gcttctcgct ggtcatgtgg ctgggcggcg ggctggacag cggattgcgc atcatcttcg
      901 gcatcgtcgt cggcggcgtt ctggccctgg tgttctacat gatggtgaag ttcatgctcc
      961 atatggccgg gggcgtcgtg ggtctggtga tcatgctggc gctgctgggc atgttccggc
     1021 tgggcgggct gaatgtgggt gcgttcggct gggtgctggc cgccgcggcc gcggtgggcg
     1081 gcggcttcat cggcaaccgg ctgggcaaca aagtggtcgt gctggcctcg gcgctggccg
     1141 gctcctatct ggtcgtgctg ggcctggggg cgctctacaa ggtgggcgtg gactcgaccc
     1201 agccgttcga aaccctctcg gcggcctttc cgctggtgct gttcctggcg atcttcgcca
     1261 tcagcttcct ggcgcaaaat caggcgcaga acttgcggct gcggtttttg cggtagcgtg
     1321 tgagaacttc acgcccgggc acgtgggcgc aagcgagaga ttatcctgct tgcgcctttt
     1381 gtgccccggc gtgttttcgc aatcgggcga ttccgccccc ctaccgccag gctttgacgt
     1441 atatctcata gttgccgctg gtgttgtcat tccaggcgac aacgggcata ttcgcgcccg
     1501 tgatggtgac cgagggcaga gatgacgacc ccagcgtgtt gctgatgccg ccacctgttg
     1561 ccgagccggc ccccacttca acccaaaagc tgccgttcca gcggcgaatc aggatgtcgg
     1621 tattggcccc cagccgctcg taccagatca catagaccgt atcatccggc gcaaccgcga
     1681 catggggggc caacgagacc tcgcttgacc cgctgatgcc gccgctcgcg gccgaacccg
     1741 cgccaacctc gacccaggca ttgccatccc aactcttcac atagatgtcg aaatgcccgc
     1801 ccgtattatc gggccaggtg acgtaaatga tccccgtcga atcaacgcca atgacgggcg
     1861 gacgcgagtc gccggcgtta ttgctgatgc cgccgccgct tgccgagccg cccacttccc
     1921 cccacgtgcc gccgctccag cgccgcgcat agacctcagt atcgcccggt gtgtcatcgg
     1981 accagccgac gatggggttt ccattctgat ccacggccag gaacggccga ccggaatcgc
     2041 cggcattgtt gctgattccg ccgccggaag ccgaccccac gccaacctcg gcccaggcgt
     2101 tcccgttcca atggcgcacg tagatctctt tatcggccgc ctggctctct tcccaggcca
     2161 catagacaac gcccgatgtt gagatgccca tcgatggcca ttccgattcg gtggccgttt
     2221 ggctgatgcc gccgcccgac gccgaccccg cgccgatctc accccaggtg ctgccgtccc
     2281 agcgacgcac gtagacctca tacccgcccg cggtcttctc ggcccacgcc agatagatcg
     2341 aaccgtccgg ggcggcctca agccagggag agtccgagat ggtggccgta ttgctgacac
     2401 ccccgcccga cgccgacccc gcgccgacct cgacccaggc gtttccgttc caacggcgca
     2461 catagatatc gctctgcttg gcgcggtcct cgacccaggc cgcatagatg tcgtcaccga
     2521 catgggtgat cgccggccgg ctggaatcat cggccgtttg actgatgccg ccgcccgacg
     2581 cggaaccgga gccaagctcg ccccaagcgc cgctaaccaa ttcattgtcg cgctgcgaca
     2641 cctgcacggt cacttcttca ccgccagggt caagctcggc gccatacagt tcgatctgcc
     2701 attggcccgc cggcggattg gcaatgcgat aaatctcgta ggtcgcgccc ttggcatgat
     2761 agatcgtcgc gtcattctgc gccgtgtcgg gggtgatgac gcggccgttg gggtcaacaa
     2821 gggtcgcatc gacgcggctg cccggccagt tgacgacgaa gttggccgta ttttggccgg
     2881 tgtcaatcgc tgccgggatg gtggccgact gcccttgttc catcacaaac gccgtctcca
     2941 caacgacatc ggaaccggtg acggccgcct gaatagcgaa gtatacctgg gacagctggt
     3001 tcgggtttgt caggctgaaa tactgcccac cggtctctga tgcgatttgg cgcagcaggg
     3061 cttcattcgc atcaccgctc aggccgattg taaagaccgg ccagcccttg tcacggtaga
     3121 gctgggcctg attgaagtat tcgccatcgc catcggtcaa caaaacggcc gccttggggt
     3181 tggcgtttgg cgaagaaaca agctggtcat agcctgtttg caaccccagg ccgatattcg
     3241 tggtgccatt gctgtcgatc gtatcaacgg ccgccagagg cgcgcttcgg tcgtttgtca
     3301 gcgtctggag atggaaagga acgcgaacgc cgccatcaaa atccacaatg gtcaacatat
     3361 ccccgctcac catggccccg atgaacacct tggccgcggt tttgcgcatg ttggccggat
     3421 cattccaggc catgctgccg gagctgtcga tgatgacggc cacatcaatc gtcttgattg
     3481 gttcgccgcc ctggcgcgtg gtgaatttcc agaccgggcc ggtggccttg ttctgggcgc
     3541 tgtcggtcgc ttcgacgcgc caatagtagg tcgtgttctc gctcaatggc cccacgtcgc
     3601 aggccaaaac ggagattgcg gaacaagcgg ccgcgtcggg ggtattgtct cccgcttcca
     3661 gaaagaccgt gtaagtcgtc gggtcgccgt cgggatcgcc cccgacccac ttgagttgca
     3721 gatagatttc ctgctgatcg ctgttgttgg ccgggatggg gctggacgga gtgaacggcg
     3781 gctggttgac gccgcccgca gtgtcgaagc gccaggccgg gccggacgtc gacgcaccct
     3841 gattatcctc ggccacgacc cgccagtaat aggtctcatt agggtcaagg ccggtgacgt
     3901 cgcagtaggc gacatgggcg gtattgcata ccatgacatc gggggagcta tccccgaatt
     3961 ccataaatac atcataggac gtcggatcgc catcaggatc gccgccggtc caggccagcc
     4021 ttaggccaag cggccgcccg gtcgcgagat gtggcgggtc cgggttggcg ggtatgaatg
     4081 gcgggtcgtt cgacgtgtcc ccgccctcgg cgatgaggat cttaatctca atgacggtgc
     4141 ggctgatatc cacaaacagt ttgctgaaga cttccagctt gacgccaacc ggcaccgtca
     4201 gaccggcgta taatttccac ttgaagggga gcagggaatc gagcgcgagc gcgccgaata
     4261 ttttcgcttc ggcatagggg ccgacgacgc cataaagctt gattgcgagc tgtggcccca
     4321 cgccggcctg gatcttgaac tgcttatcga ccgtgacggg caaagggctg aagtcgtcgg
     4381 atgcatcgcc gaccatcccc caattgccgt tctcaagccg caagccaaac tccatatggg
     4441 ttgaatattg gacgccaaat gtcgtttcgg cttccaccga gccatcaacc cccaggacaa
     4501 tggccagttc aggagtgaac acaatcggga caaaaccaac catgaccgtg attggctgga
     4561 acgtgtgtcg ggcgaggact tttttcttct cgacgagcgt tgcacccagg ccgaccgacg
     4621 ccttgtatct caattcggaa ttggtgtccg atgtgatgat gaagtccatt ttctccagac
     4681 ttcctccgcg aatgtcgaag ccgaagtcga tatcctggct aaccgccacc gtgccatcgg
     4741 ccgtaaactg gtcgtttttg gtggcgtcgt tgccatccct gtcgtagatg acgaattcat
     4801 cgagggtgta ggagaaggta tcggccgcca tgccgcttcg gtcggatggg gccatgctta
     4861 cgccgggcat caggatggtc gatgcgacct tgtccggcga cagggtcagg gacaagtcga
     4921 ggctgccgtc ggtaattgcc tcatcgaggg tcgccggggt ggttgtgaca atccgcttgt
     4981 tgccggtgtc ctgcagcccc gtcacctttc gcagaaagcc atccggggcg gccgcagtgg
     5041 gcgcgccaac catgacgtcg ccgatatcca gatcatccag cgcggcgggc gaaccatcga
     5101 aaacaaactg ttcgccgtcg gccgacacgc tttcaagttg tgccagcgtc tggcccgtga
     5161 tgacttccgt ggtttcaggg atagtgactt gctgatttcc atttccccca atggtcatcg
     5221 gcaaatagat cgggaaggac tgggctttgg cttctttcga tacccaagcc gacagaccac
     5281 tcaggacaac caggcctacg agcgcagtga gcagccgaac gtatcgagta tgcacttttc
     5341 taccccctgg cgagacggtg atgcctctcc catcccgcat caatcaccag ccggcttcaa
     5401 cgtataaccg cttaacccaa aagggttata gggaacaacc ctatcatacc cgaattcccg
     5461 ttcaatccaa gagaaagatc aattacacga taccccccaa ctcatcccac cgcgccagat
     5521 agcgcgccag gtgcgtttgc cccgcggcga tgcgcttgcc gatcaactcg cggacgcgct
     5581 cgacctcttc ggcgtggccg gcgtcgttga aatgcccggc gaagtgggcc gcacgcagcc
     5641 atagcaggta ggtcgtcagg gcgtcgaact cgttgtattc gacgatccgc cgcacgtcgc
     5701 ccgccagcca caggtcgatg acgttgtcgc cgctggtgtc gatcttgccg gggatgccgc
     5761 aggcggtggc gaactggtgg agactgggcg tggccttgcc ccaaccaccg acaacttcct
     5821 tcaggtcgat gtgcccctcg ttgtatttgt cgaaataatc gaacccttcc cagcgcttct
     5881 ccggccggtg ggcgaagccg ggcgcgctca cgccctgggc catgccgcgc tggatgagga
     5941 tgggcaggtc ggactcacga gagttgaagc cgacgagctg cggccgcggg gtggcgtcgc
     6001 cgatgccctt cagaaagcgg ccgattagtt cgcgctccgg catggcctcg ctctggggca
     6061 gcgagaacag cgtcagcgac acgccgccgc cctgcacctt gcgcatgacc acggccagcg
     6121 acacgacccg gcacagcatc gtcttcagat agggccgcgg ctcctcggcg gtggctccgg
     6181 cgtcggccca catggcggca tagacttcgt cgtcgggcgt ggcggcgggc agattacagg
     6241 cgcggcggcc ggaagcggag tctggcaccc actccaaatc gaatgcccag acatgatcgg
     6301 gaacggcacg aaaaatagtc acaagcgatg cctcctgagg gattggaagg tattttagcg
     6361 catcttttta gaaaccgggt ttcttccgag aaacccggtt tctacgtctc gcggaactct
     6421 ttcacgcctt atctacgcga aacacaccgg ccgtttacgc gccccgcgac tcggccggtg
     6481 tatattatgt tcagacagca acgatagatt tcctcctatc cccttccgag acgccgcgac
     6541 tcccccaaac gcggcgtctc ccctttttcc acattcgcat tcccgtacag acccgctaaa
     6601 cttgaggccg agtttggtca ccagtcagtc gtaccattgg ccccggcctg tttccatggc
     6661 ctgatgtggg gtcgtcgctg ccgaagttgt ggagtcgctc atgaacgtgc ccgccatgcc
     6721 gcgatgggta ttattgatcg ttgttctgct gcttctggcg gctgctcggc cgttcggccg
     6781 cctgttggcc cagagcagta cgctgtacgg gacgctggag atcgtctggg gcgatccgca
     6841 aaccggcggc gcggccgtca cccattacta cctgaccacc gacgacggcc gaaccgttcg
     6901 cctcgtcctg ccggagacga tgaccgtcga tctcctggcg ctgaacggcc gccgggtcat
     6961 cgtcgaatcg cgcggtccca cccggaacgg caccggcagc gggcagccgg agctacaagt
     7021 ggacgccatc cgccccgccg atccatcccc aatcgccccg gccccgctgg tcgaaggtgg
     7081 tcagcccaac gtcaccctcc tgtgcagctt ccccgacgtg gccgccaccc acaagccgct
     7141 gagctattac caggagatgt accgcgacac ggcccccggc ctgaatcatt actggcaaga
     7201 cctctcctac ggcctggcca acgtcgcggg cagcgccgcc tttggcccct atgccatgcc
     7261 caacccgttg gccttttata tgaacaatgg cgaagccgac ctcacccaac tggcgaccga
     7321 ttgcgtcgcc gcggccgacg ccacagttga ttttccccaa ttcgagggca tcaacatggc
     7381 cttcaacgcc aacatcggct gttgcgcctg gggcggcaag cgaacgatga cgctcgacgg
     7441 cgtcaccaaa acctatgcca ccatctggct gccgccgtgg gcgagcaaca ccatcagcgt
     7501 cgtcatgcac gagatgggc
//
LOCUS       DAOMQG010000145         7433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4275_length_7433_cov_10.985769, whole genome shotgun sequence.
ACCESSION   DAOMQG010000145 DAOMQG010000000
VERSION     DAOMQG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_7433_cov_10.985769"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PLC06_09225"
     CDS             complement(<1..1795)
                     /locus_tag="PLC06_09225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90665.1"
                     /translation="MRPFFNPRLVALIGLFVALGLTYNVAVPLFEAPDERDHVDYADW
                     LAGGNGLPHLAADRGEVGEIWQPPLYYALIGALIAPIDRTGLATIAPLSEDWLAGLSR
                     VAHYHTSAEAFPYRGGASAVHVARGVSTLLGLITILATYAIARRLIPRHALPAAALVA
                     LNPQFIFMSAAVNNDNLVIALSSLALWILVILIDEADKAAGRERQVFWFGALGLVWGL
                     AGLTKLTGLTLGLPIGLTLFYLAWRGRSWRPLLVGGVVTGGAMLLICGWWFWRNWQLY
                     GDPLAWKEMLSVTGALVRPELLSWPETLSYAGFLRFSYWAAFGYGVSAPATFYLMTGA
                     IMLLGIVGWVMIAVRRPWRNMPPARFTLLILFVWSLTVFVFLLRWMRQIDATNQGRLL
                     FPAIASLSVLLAVGLAALDGRRLWLARIVTVALGCWAAAMPLLVIVPAYAQPALVDAS
                     AIANPAEIRFGDGIRLLGYELPAVAEPDQPLEMALYWETDRALETSYVIALRILDSTG
                     QPVSGIDTLPSGGRYSTKVWEPGRPFRDTYQLPPVAADAAPGRGMLLVTLYPRGEPDM
                     PLPVSAFDVMVGNEALLGAIKLPPIKPMTAEP"
     gene            1995..4220
                     /locus_tag="PLC06_09230"
     CDS             1995..4220
                     /locus_tag="PLC06_09230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90666.1"
                     /translation="MQWRRHPLPIITAAFVALAALYVWAIPPLEGSDEFEHFAYVTWL
                     IEQRSFPIQGEAGWNTPVRQESGQPPLYYFLASLPARLAGTQPPVVFRPNPYFRYDLD
                     PTRPDNKNTALHYPADTGGGWSALYLARTVSVLSGILLIVFIYKLTETVLPGRPGVAA
                     AASAFTAFIPQVIFHSSHVSNDILAAAFSTAALWQLARIVRFGGSRRRGFALGMFLGL
                     AALVKVHTLLIGLPVALGFGWLLLGRGSIKQGESFRETWVTATGAALGFLVTAGWWFV
                     RGWLRYGSFLGLDTHCYQELSTCGPFRLVWPNWFAWRDTFRSFWAAFGLANIRPYDWV
                     YLVFAGLVILAAAGLGLLVIRRVQARKTPSANPMNPPADPHLPVLLILLGSAIAGNVI
                     LLYLWMQQILATYGRLLYPSLGAIVVLIIAGLWELRPRLARLAWVLPAILAVVSPFWL
                     IRPAYELPRFLDEAALTARGESLGWRYGDVAELLSVTPATRSAAAGETLMVELCWRTL
                     APSATNYTVYLQAIGPGESVVADRYTYPGLGSYPTAIWEPDKVFCDEVQLPIPADLPH
                     TLAYRLSVGLLDDQTDERLPGTDSAGNPLPPFAGMVRLAATTEPTDERPPSGDAAIRP
                     ATAEFESVWRPGETQPITLRWYAAETVPADYTVFLHLRDDTNQLIAQADGPPLDGWYP
                     TSWWQAGERVTDTHSFLVPDNIPPGIYHLVAGLYDPISDGRLGDEQHLGTVEIVTGDN
                     P"
     gene            complement(4258..4860)
                     /locus_tag="PLC06_09235"
     CDS             complement(4258..4860)
                     /locus_tag="PLC06_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90667.1"
                     /translation="MYEENERLGQDAAVLSAMAEHMPEYLDSDILFWPAPRGGMPALT
                     LGGYLLREHRLLALAGLLTAEQQMEVNEALTRFNQALANRVVRFEKKAHHELEARLRQ
                     WEEYLKDMDRGTFDRSSNYATAVEARAIIQALLNRLGMPPYRTEARPEQHLATLDTRL
                     RGRWQPGDFVWPAAWEPAYPREAYWWLYGSPRVTERDPNS"
     gene            4971..5273
                     /locus_tag="PLC06_09240"
     CDS             4971..5273
                     /locus_tag="PLC06_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90668.1"
                     /translation="MTKQMRRAAIYKLASAAHEMELDVMSGVLARDEDGHWRIGDHDL
                     IHWLESHTGEELVLVLGSLADDRDVQVRTCRTCGRDYMELECPYCRANRIRLRGHA"
     gene            5339..6247
                     /locus_tag="PLC06_09245"
     CDS             5339..6247
                     /locus_tag="PLC06_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRO90669.1"
                     /translation="MVILVSLGVAAPIAAQGNPAQLTSLAVELWPDYDRPSMLVLLTA
                     TLPQDTPLPATLTLPIPAGAEIHAVASFNATGALMSDVDYTVENGQITLTTPANRFRV
                     EYYSPYQVEDGKYSYTFKWVSDLAIEQVTTVVQQPVAAREFRIDPAATSSAAERGDGL
                     TYHTLPARSVSAGEPFTVTITYAADAPILSAPSQTAPAANTDTTTAAPATPAAGFDPV
                     WLLVGAGVLALVGGAWYIGQRQGRSASRSRKPQPTRAAKPNAGDTKRTSDAPRASSTT
                     KFCHNCGRQSQPGDTFCRNCGAQLKD"
     gene            complement(6356..7207)
                     /locus_tag="PLC06_09250"
     CDS             complement(6356..7207)
                     /locus_tag="PLC06_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRO90670.1"
                     /translation="MQIKIVIGTIAFMIAMMVLGYSALREPARLGRFTNAEVGRSIEA
                     GSEIFANNCTTCHGTDGTAQNCVDSTGNQIACQGLPLNYNALLCGDVSQRMTDMNWSG
                     TKANYVLTTISAGRGAMPTWHEQFGGPLRPDQVQNVANFVLNWESETLCSAPVVTYEW
                     PDQVTDFLSGPDVTSPGDATRGADLYAVTYGCAACHGAHDGSTPAAIGPSLIDIKTNG
                     ATRVDGMDAQQYVYHSILFPSDFIAPECPTGPCAGPPSVMPANFASRMGDNPQDMADM
                     LAFLLGE"
BASE COUNT         1448 a         2442 c         2152 g         1391 t
ORIGIN      
        1 gcggttcagc cgtcatcggc ttgatcggcg gcaacttaat tgcaccaagc aaggcctcat
       61 taccgaccat cacgtcgaag gcgctcacgg gcagcggcat gtccggttcg cctcgcgggt
      121 agagcgtcac gagcaacatc cctcgcccag gagcagcatc cgccgccacg ggcggcagtt
      181 ggtacgtatc gcgaaacggc cgccccggtt cccagacctt cgtggagtag cggccgccgg
      241 agggcaacgt gtcaatcccg ctcaccggct gcccggttga gtcaaggatg cgcagggcaa
      301 tcacatagct cgtctccagc gctcgatccg tctcccaata gagcgccatt tccaatggtt
      361 ggtcgggttc ggccacggcg ggcaactcat aacccagcag gcgaattcca tcgccaaacc
      421 ggatctcggc cgggttcgcg atggcgcttg catcgaccaa agccggctga gcataggcag
      481 gcacgataac gagcaggggc atggccgccg cccagcaccc cagcgcaacg gtaacgatcc
      541 tggccaacca caagcggcgg ccgtccagtg cggccagccc cacagccaac aacacgctca
      601 gggaagcgat ggccggaaag agcaagcgtc cctgattcgt cgcgtcgatc tgcctcatcc
      661 agcgcagcag aaaaacaaag actgtcaggc tccagacgaa caggatcaac agggtgaacc
      721 gggcaggggg catattgcgc cacggccgcc gtaccgcgat catcacccag ccgacgatcc
      781 ccaggagcat gatcgcgccg gtcatgaggt agaacgtcgc cggcgcgctc accccatagc
      841 cgaaggcggc ccaatatgaa aagcgcagga acccggcata gctcagcgtc tccggccagg
      901 acagcagttc cggccgcacc agagcgccgg tgacgctcaa catctccttc caggccagcg
      961 gatcgccgta aagctgccag ttgcgccaaa accaccagcc acagatgaga agcatcgctc
     1021 caccggtcac caccccgccc accagcaaag gccgccagga tcgccctcgc cacgccaaat
     1081 agaacaacgt caggccaata ggcaagccaa gggtcagccc cgtcagcttg gtcaatcccg
     1141 ccaggcccca gacaagcccc agtgccccaa accaaaatac ttgtcgctcc ctgcccgccg
     1201 ccttgtccgc ctcgtcgatc agtatgacca gaatccacag cgccaggctg ctcagggcaa
     1261 tgaccagatt gtcgttattg acggccgcgc tcatgaaaat gaactgcggg ttcagggcca
     1321 ccagcgcggc cgccggcaac gcatggcgcg ggatgaggcg gcgagcgatg gcgtaagtcg
     1381 ccaggatggt gatcagcccc aacagggtcg agacgcctct ggcgacatgg acggccgaag
     1441 ccccgccacg gtaagggaac gcttcggcgg acgtgtgata atgggcgacc cggctcaacc
     1501 ccgccaacca atcctcgctc agcggcgcaa tggttgccag acccgtccga tcaatgggag
     1561 cgatcaacgc gccgattaag gcatagtaga gtggcggctg ccaaatctcg cctacttcgc
     1621 cccgatcagc ggccagatgg ggcaagccat tgccgcctgc cagccaatcg gcgtaatcga
     1681 cgtgatcccg ctcgtcgggc gcttcaaaga ggggaacagc tacgttatag gtcaggccca
     1741 aggctacaaa caagccgatg agcgcgacaa ggcgcgggtt aaaaaacggg cgcatggtga
     1801 atgggattaa tggctcctgt cgtttcccct tttatatccc gttggtcgct ttttgacacc
     1861 gtcaaggatg gggcaaggcg cgattatgcc gaaagggcgc ggaaaggcgg agttctctca
     1921 taccgttctt ctctctaaag cgatgatggc cggttaccat acccaagcgg cgcatcaacg
     1981 gtacaattcg gacaatgcag tggagacgac atccgttacc gatcatcacg gccgcttttg
     2041 tggcgctggc cgcgctctat gtgtgggcca tccccccgct ggaaggctcc gatgaattcg
     2101 agcatttcgc ctatgttacc tggctcatcg agcaacgcag tttcccaatc cagggcgaag
     2161 cgggctggaa cacgccggtt cgtcaggagt caggccagcc gccgctctac tatttcctgg
     2221 cctcgctgcc ggcgcgattg gcgggcacac agccgccggt cgtgtttcgc ccaaaccctt
     2281 acttccgtta cgatctggac ccgacacgac ccgacaacaa gaataccgcg ctccattatc
     2341 cggccgacac cggtgggggt tggtcggcgc tctatctcgc ccgaacggtg tcggtgctct
     2401 ccggtatctt gctcatcgtt ttcatctata agctaaccga gaccgtattg cccggccgac
     2461 ccggcgtggc cgcggcggcc tcagctttca cggccttcat cccccaggtc atatttcata
     2521 gcagccacgt ctccaacgac atcctggccg cagccttcag caccgcggcc ctctggcaat
     2581 tggcccgaat cgtccgattc ggcggcagtc gccggcgggg ctttgctctt ggaatgtttc
     2641 tggggcttgc ggcgctggtc aaggttcaca cgctgctcat cggcctgccc gtcgccctcg
     2701 gtttcggctg gctgttgctc gggcgtggga gcatcaaaca gggtgaatct ttccgcgaga
     2761 cctgggtgac ggcgaccggc gcggcgcttg gctttctggt gaccgccgga tggtggttcg
     2821 tgcgtggctg gttgcgttac ggctccttcc tggggctgga tacccattgc tatcaggagc
     2881 tttccacctg cgggccgttt cgtctcgtgt ggccaaactg gtttgcctgg cgcgacacgt
     2941 tccgctcctt ttgggccgcc ttcggcctgg ccaatattcg cccctatgat tgggtctacc
     3001 tggtttttgc cgggctggtg atcctggcgg ccgccgggct ggggctgctg gtcatccgtc
     3061 gagtgcaagc ccgcaagact ccctcagcca acccgatgaa cccgcccgcc gatccacacc
     3121 tgcccgtcct gctgatcctg ctgggcagcg ccatcgcggg taacgtcatc ctgctctatc
     3181 tctggatgca gcaaatcctg gccacctacg gccggctcct ctacccgtcg ttgggggcca
     3241 tcgtcgtcct gatcatcgcc gggctttggg agctgcggcc gcgcctggca cggctggcgt
     3301 gggtgcttcc cgccatattg gctgtcgtat ctcccttctg gctgatccgg ccggcctacg
     3361 aacttccgcg cttcctcgac gaggcggcat tgactgcccg aggcgaatcg ctcggctggc
     3421 gctatggcga tgtggccgaa ctgctcagtg tgaccccggc aacccgctcg gccgcggcgg
     3481 gcgaaacgtt gatggtcgaa ctatgctggc gaacactggc cccgtccgca accaactaca
     3541 ccgtctacct gcaagccatc gggccgggcg agtccgtcgt tgccgaccgc tacacctatc
     3601 ccggcctggg cagctacccg accgccatct gggaacctga caaggtgttt tgcgatgagg
     3661 tgcagctgcc catccccgcc gatttgccgc acacattggc ctatcgtctg tctgtcggtt
     3721 tgctggacga ccaaacggac gaacgcctgc ccggcacgga tagcgccgga aatccgctgc
     3781 cgccgtttgc gggaatggtg aggctggccg cgactacaga accgacggac gaacggcccc
     3841 cgtcgggcga tgccgcaatc cgtccggcca cggctgagtt tgaatctgtt tggcggccgg
     3901 gcgagacgca gcccatcacc ttacgttggt acgcagccga gacagtcccc gccgactaca
     3961 ccgtcttcct ccatctgcgc gacgatacta atcagctgat cgcgcaggcc gacggcccgc
     4021 cgctggatgg ctggtacccc acatcgtggt ggcaggccgg ggaacgggtg acggataccc
     4081 actccttcct tgtgcccgac aacatcccgc cgggcatcta ccacctcgtg gctgggctat
     4141 atgatccgat aagcgacggc cgtcttggcg acgagcaaca tctcggcacg gtggagatcg
     4201 tcaccggcga taacccgtga gccggcgctt atgttgcaat ctgtgagcgc ctttccgtca
     4261 ggagttcggg tctcgctccg tgacgcgagg gctgccatag agccaccagt acgcttcgcg
     4321 cggataggca ggctcccacg ccgcgggcca aacgaagtca ccgggctgcc agcggccgcg
     4381 cagacgtgtg tccagcgtcg ccagatgctg ttccggtcgg gcctcggttc gatagggggg
     4441 catgccgagg cgattcaaca aggcctgaat gatcgcccgc gcctccacgg ccgtagcgta
     4501 attgctggac cgatcgaacg tgccgcgatc catatccttg agatactcct cccattggcg
     4561 aagacgcgcc tcaagctcat ggtgcgcctt cttctcgaag cgaacaaccc gattggccaa
     4621 tgcctgattg aaccgggtca gcgcctcgtt cacctccatc tgctgctcgg ccgtcagaag
     4681 cccggccagc gccaacaaac gatgctcgcg caacagatag ccacccaaag tcagcgcggg
     4741 catcccccca cggggagcag gccaaaacaa tatgtcgctg tcgagatatt cgggcatatg
     4801 ctcggccata gccgacaaca cggccgcatc ctgtcctaga cgttcattct cttcgtacat
     4861 ggaagcctca ccttgtcgtt ttagttcatc ggatataata gaccttggtc ttgatggcgt
     4921 caaacgttgg caagcccgtg ccgaacagat cggcgcaagc gaggatgaca atgacgaaac
     4981 aaatgagacg cgccgcgatc tacaagctgg cttcggccgc ccatgagatg gaactcgatg
     5041 tcatgagcgg ggtcctcgcg cgcgatgaag acggccattg gcggatcggt gatcacgacc
     5101 tgattcactg gcttgaaagc cacaccggcg aagagctggt gctcgtgctc ggttcgttgg
     5161 ccgatgaccg ggacgttcag gtacgaacct gccgaacgtg cggccgcgac tatatggagc
     5221 ttgaatgccc ctattgccgt gccaaccgca ttcgcctgcg aggccatgcc taattctgtt
     5281 atgttaactt gactatcgtt ttatgcacca aataaatccc cttctcaccc gtctattcat
     5341 ggttatattg gtctcgctcg gcgtggccgc gccaatcgcc gcccaaggca accctgcaca
     5401 actaacctcg ctggccgtcg aactctggcc cgactatgac cggccatcca tgctcgtcct
     5461 gctgacagcc acattgccac aggatacccc gctcccggcg acgttgactc ttcccatccc
     5521 ggccggggcc gagatccacg ccgtggccag cttcaacgcg accggggcct tgatgtcgga
     5581 cgtggattac acagtcgaaa acgggcagat cacgctgacg acgcccgcca accgcttccg
     5641 cgtcgagtac tattccccct accaggttga ggacggcaaa tattcctaca cgttcaaatg
     5701 ggtctcggat ttggccatcg aacaggtgac aaccgtcgtc cagcagccgg tggcggccag
     5761 agagtttcgg atcgacccgg ccgcgacttc ctcggccgcc gagcgcggcg acggcttgac
     5821 ttaccacacc ttgcctgccc gctccgtgag cgcaggcgaa ccatttacgg tcaccatcac
     5881 ctacgcggcc gacgcgccga tactatccgc cccatcccag acggcgcccg ccgcaaacac
     5941 cgacacgaca acggccgcgc ccgccacccc ggccgccggc ttcgatcccg tctggctgct
     6001 cgtcggggcc ggcgttctgg cgcttgtcgg cggggcatgg tacatcggcc aacgccaggg
     6061 ccgctccgcc tcccgctccc gcaagccgca gcccactcgc gcggccaagc ccaacgccgg
     6121 cgacaccaag cgcacgtccg acgcccctcg cgcatcatca accaccaaat tttgtcacaa
     6181 ttgcggccgt caatcacaac cgggcgacac cttttgtcgc aactgcggag cgcaattaaa
     6241 agactagatc gagaccggaa tgatattcat ctgatccaca agggttgatg aatataaaaa
     6301 ggcccgtcct gaatatcagg gcgggccttt tttatacgct tacagctcga ctaacttatt
     6361 cgccaagcag gaaggcgagc atgtcggcca tatcctgcgg gttatcaccc atgcgtgacg
     6421 cgaagttggc gggcatgaca ctgggcggcc cggcgcacgg ccctgtgggg cattccggcg
     6481 cgatgaagtc gctggggaac aggatcgagt gatagacgta ttgctgggcg tccatcccat
     6541 cgacgcgggt cgcgccattc gtcttgatat cgatgagcga cgggccaatc gcggccggcg
     6601 tcgagccatc gtgcgcacca tggcaggcgg cgcagccata cgtcaccgcg tacagatcag
     6661 caccccgcgt cgcgtcaccc ggcgaagtca catccgggcc gctcaggaaa tcagtgacct
     6721 gatccggcca ttcataagta acaaccggcg cagagcagag cgtctcgctc tcccaattga
     6781 ggacgaagtt agccacattc tgcacctggt cgggtcgcaa cgggccgcca aactgctcgt
     6841 gccaggtggg catggccccg cggccggcag agatggtcgt cagaacatag ttggctttcg
     6901 tgccactcca gttcatgtcg gtcatacgct gcgacacgtc gccacacagc aaggcgttgt
     6961 agttcagcgg cagaccctgg caggcaattt gattgcctgt ggagtcaacg cagttttggg
     7021 ccgtgccgtc tgtgccgtgg caggtcgtgc agttgttggc gaagatctcc gatccggcct
     7081 caatcgagcg acctacttcg gcattggtga aacgtcccaa acgggcaggt tcgcgcaacg
     7141 ctgaatagcc gagcaccatc atcgcgatca taaaggcgat agttccaatg acaattttaa
     7201 tctgcatctg tcatcattcc tctttcgtca ggtagagaca tcccgacggg acgtctctac
     7261 cggggcggca attaatcagt tgcacaggac ttgttcagga agcggcattc ctcttcataa
     7321 ttggagaatg cgtgccgcgg cctggtccac tccgtctttg aaaccgcgcc tgtgtcagga
     7381 ttggtaatct cgtagtgcaa gatcaactct ttcaggttcg gctgaacttc gac
//
LOCUS       DAOMQG010000146         7373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4318_length_7373_cov_5.462831, whole genome shotgun sequence.
ACCESSION   DAOMQG010000146 DAOMQG010000000
VERSION     DAOMQG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4318_length_7373_cov_5.462831"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLC06_09255"
     CDS             <1..374
                     /locus_tag="PLC06_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90671.1"
                     /translation="YSSNIPTGALYSPLKMDSISEFSAGIWANPLYRKLILSMLVVLG
                     TFLLQIIARQVLVKRLTRLSGETTHTCTLRKIIDYVTSETAPVSCGLWSRALASTRQT
                     GEQITRTGPDCLAPTCQILQF"
     gene            528..3626
                     /locus_tag="PLC06_09260"
     CDS             528..3626
                     /locus_tag="PLC06_09260"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90672.1"
                     /translation="MHANRWLKLSLFSLLFVALPLMAMQQPVRTPLEDAPTLPNDFMP
                     AAPTADLQPGAVTDASYEVAPILFVPAGVTPVSNGLDYVNRTMDNVRHWYAEILDGHT
                     FSLTPAELVIGQHPLTYYFGNCYPPVDSSACNLGATMWSNVFAELDSRGYILPANLVQ
                     GIFFQGEGFSTALGGGRRFLVGFNPDSLFPDCLQPGCANNVSLGGAAHELGHAFGLPH
                     PDGDPQYGVSVMGVGLYSFPQTTFINSPAFPEYDQLLASPFINVDLPLSNPGFEGCLT
                     GWNVTKGAPSCVQSGQLSGLNALHLDSAGRTRLNQAFELTPGGVYDFSGWVNAATLPA
                     GASITIQLDALRANSTVAQTFTITEIDQSTDGWTRVAASLQLPADASKGELWISTQQV
                     AGIDATLDDLNLASPAATLPAPLIATAFDGDAVATDRPLLHWSPVASAVSYDVQVAAD
                     PFFESPLADASGIVNFAYQPPALPYNMYSYWRVRAVNGAGVGDWSPVWSVIPREPADF
                     YNDEFEASTLSDAWSFVREDPAHWRIGGIAGRRWDGYFVVDLQPGDLEMTNDARNLLL
                     RAMPQGDLSLETLVNFWYPLSANYQQGGLIIYQDDDNYIRLGHVFRDGMRIEYEAEIN
                     GVIQERSDAWMVDTPRLRIERRGNVYTAAYSASGVDWTPLGQPVTVDWPNARMGLAAY
                     AGPATQLPSAALNWFRVTDPCLAVTVASDPVGSGSVSTAGAGCGDGRFRPGSPLRLTA
                     TAAPGYAFDHWSGAAAGDANPLEIDATGDLAVTAHFRTVNPAAPDLYLSPEQPGNVGG
                     IAITPQDIIKRDGATGAWSLYFDGSDVGITRPIAAFARLQNGDLLLVFRSRQVIADVG
                     TFNVADVVRFSPITTGEVTEGSFHWYFDGSDVGLQGGREQIDALEALPDGRLLISTTG
                     TAAVPNGGNVVRAQDEDLLAFTPTTLGETTAGSWALYLDGTAIPGLARKDVSGAAVDS
                     ANGDIYITIDGNYRVGGVRGNGKDILLLHPVGDGYSVARYWHGPDDGFRPNIGGLEIG
                     GD"
     gene            complement(3709..4353)
                     /locus_tag="PLC06_09265"
     CDS             complement(3709..4353)
                     /locus_tag="PLC06_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO90673.1"
                     /translation="MSPTPVLPKTKRTQRPYPGGEPVWDLAYLYPPQGKWSEEAYLAL
                     PGGMQVEYKDGFVEVLPTPTMAHQFIVLYLYRLLNDYVMAHRLGWVLVAPLPVRLRPR
                     VYREPDVVFLSADRPERLAEDYPDGADLVMEVVSGGREDRRRDWVVKRAEYAAAGISE
                     YWIIDPDERIITVLRLEGGEYIEQGRFTDEAEAPSALLPGFAVPVTEVWAAAAG"
     gene            complement(4411..6231)
                     /locus_tag="PLC06_09270"
     CDS             complement(4411..6231)
                     /locus_tag="PLC06_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRO90674.1"
                     /translation="MALSSDAVITLIILTAAIVLFLTNRLRADVVALLVLVSLGATGI
                     LTPQEAFSGFSRSAVVIIVTIFVLAQGLNLTGAGEKVGDWLIRVAGRSEGRLVILVMI
                     AGAFLSLFMNNIAAAAVLLPGVSGAANKQGTNPSRVLMPLAFATILGGMATLFTTTNI
                     VTSGLLREQGLPGFGVLTFLPLGLPIAAVGIAYMAAFGRRLLLHRPAAEVLNRPLATR
                     SEDLVEVYQLDERLFRARIPPGSMLEGRSLAQSSLRERFNVNVLSLERNGQIRLAPPP
                     DTLLQVGDVVMLEGSLEEFRERDIQPYLEILAPKRIEERDLESEGIIVVEAALAPRSG
                     LFGKTLQSVSFREQFGYSALAVWRGNRPIRRGMTTLPLQLGDALFLQGPRNHLRLLQQ
                     NPDIILLDVPSGAEPTVRSDKLALAVVIVVITLAISATNIIPTAEMMLVGALAMVLLG
                     VLSADEAYGAIEWKAVVLVAGMLPMGLALTNTGLADLAAAKIVALAAPYGPRALLAAL
                     FILTTLLAQVISGPAVAAIVVPLAISAARQTGIDPQSLSLGVALATSMAFLTPLGHPV
                     NILVMGLGGYRFADYFKVGLPLTILLTLLILLTLPLVYPL"
     gene            complement(6266..>7373)
                     /locus_tag="PLC06_09275"
     CDS             complement(6266..>7373)
                     /locus_tag="PLC06_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRO90675.1"
                     /translation="KKRTKINLSQPIQPRGGDLEQLFATEGDAEQAAGLQLLAVRVDA
                     IDPDPDQPRSTFNDDSLHELAESIRQDGVIQPIEVTQSSPGRYLIVHGERRWRASQLA
                     GLATVPAVVRRRDYDDVTRFVRQVVENIQREDLNDVDRAAALLRLRKLMQEELDAAEQ
                     QAGEPWGGKITWAKVGGRLGYSRQRIHQLIQLLDLPDEIKDAVRDGSLSERDTRVYQG
                     LTAVQQRALHRARMDGALNAVEVRQLARLLREAPEMTVAAAARLLEQTADDELAPQEA
                     DPITPAPPPPGSPAPLPNELEPRGATFTSDPLRPARVGAPTSIERLDWVRGHLARVDR
                     HSLTAAERKELLRLLKLIADDVASLMAALKSTDF"
BASE COUNT         1262 a         2399 c         2433 g         1279 t
ORIGIN      
        1 gatacagtag caatatcccc acgggcgctc tatactcacc gctcaagatg gactctatca
       61 gcgaattctc cgccggcatc tgggccaacc cgctctatcg caagctcatc ctgtccatgc
      121 tggtggtgct gggcaccttc ctgctgcaga tcatcgcccg gcaggtgctg gtgaagcgcc
      181 tgacgcggct cagcggcgag acgacccaca cctgcaccct gcgcaagatc atcgattatg
      241 tgactagtga aacagctcca gtatcatgtg gtttatggag ccgcgccttg gcctccaccc
      301 gacagacggg cgagcaaata acccggacag gcccggattg tctcgcgccc acctgtcaga
      361 tcttgcaatt ctaatccaaa tctgacctcg acgatctatg ctcgatccgt tgatcgattg
      421 gtcgccgctc ggcgacggcc gttattcggc gaacgggacg aatctcggcc ccatccggtg
      481 gggcagcgca gtatcccacc ccgcgaacca gccacaagga gttaatcatg catgccaacc
      541 gttggctgaa actttcgctg ttcagtctgt tgttcgtcgc gttgcccctg atggcgatgc
      601 agcagcccgt tcggacgccg ctcgaagatg cgcccacgct gcccaacgac ttcatgcccg
      661 ccgcgcccac ggccgatctg cagcccggcg cggtcacaga cgcaagctac gaggttgcgc
      721 ccatcctgtt cgtccccgcc ggcgtgacgc ccgtgtccaa tggcctcgat tatgtcaatc
      781 gaacgatgga caacgtccga cactggtatg ccgagattct cgacggccac acgttctccc
      841 tgacgcctgc cgagctggtc atcggccaac acccgctcac ctactacttc ggcaactgct
      901 atccgccggt ggattcgtcg gcctgtaact tgggcgccac catgtggagc aacgtgttcg
      961 ccgagctgga tagccgcggc tacatcctgc ccgccaatct ggttcagggg atcttctttc
     1021 agggggaagg cttcagcacg gcgctgggcg gcggccgccg tttcctggtg gggttcaatc
     1081 ccgatagcct gttccctgat tgcctccagc ccggctgcgc caataacgtg agtctcggcg
     1141 gcgcggccca cgaactgggg cacgcattcg gcctgccgca ccccgacggc gatccgcaat
     1201 atggcgtttc agtgatgggc gtgggcctct acagttttcc ccagaccacg ttcatcaata
     1261 gccccgcgtt ccctgagtat gaccagctat tggcgtcgcc cttcatcaat gtcgatttgc
     1321 cgttgagcaa ccccggcttc gagggctgcc tgaccggctg gaacgtcacc aagggcgcgc
     1381 catcctgcgt ccagagcggc caactgagcg ggctgaatgc gctgcatctg gacagcgcgg
     1441 gccgcacccg gttgaatcag gccttcgagt tgacccccgg cggcgtctat gatttctccg
     1501 gctgggtcaa cgccgccacg ttacccgccg gcgcgtcgat caccatccag ctggatgccc
     1561 tgcgcgcgaa ctcgaccgtg gcccagacct tcaccatcac tgagatcgac caatcgaccg
     1621 acggctggac gcgcgttgcc gcctcgcttc agctgcccgc ggacgcgagc aagggcgagt
     1681 tgtggatctc cacccagcag gtagcgggga tcgatgccac gctggatgat ctgaacctcg
     1741 catcgcccgc ggcgacgtta cccgcgccgc tgatcgccac cgcattcgac ggcgacgccg
     1801 tggccaccga tcggccgctg ctgcattggt cgccggtggc ttcggccgtc tcatatgatg
     1861 tgcaggtcgc cgccgatccg ttcttcgaat cgcctctggc cgacgcgagc ggcatcgtca
     1921 atttcgccta tcagccccct gctcttcctt ataatatgta cagctactgg cgggtgcgcg
     1981 cggtcaatgg cgcgggggtt ggcgactggt cgcccgtatg gagcgtcatc ccgcgtgagc
     2041 cggccgattt ctacaacgat gagttcgagg cgagcacgtt gagcgacgcc tggtcgttcg
     2101 tgcgcgaaga cccggcccac tggcgcatcg gcggcatcgc cggccgtcga tgggacggat
     2161 acttcgtcgt cgatctgcaa cccggcgatc tggagatgac caacgacgcg cggaacctgc
     2221 tgttgcgggc catgccccag ggtgacctct cgctggagac gctggtcaac ttctggtatc
     2281 cgctcagcgc caattatcag caggggggtc tgatcatcta tcaggatgac gacaactaca
     2341 tccgcctggg ccatgtcttc cgcgacggca tgcggattga gtatgaggcg gagatcaacg
     2401 gcgtcatcca ggaacgcagc gatgcgtgga tggtggacac tcctcgcctg cgcatcgagc
     2461 ggcgcggcaa tgtctacacc gccgcctaca gcgcgtcggg cgtggactgg accccgctgg
     2521 ggcagccggt cacggtcgat tggccgaacg ccaggatggg gctggcggcc tatgccgggc
     2581 cggcgacaca gctcccctcc gcggccttaa actggtttcg cgtgaccgac ccatgcctgg
     2641 ccgtcaccgt ggcaagtgat cccgtcggtt cggggtctgt ctcaacggcg ggcgcgggct
     2701 gtggcgacgg ccgcttccgc cccggttctc cgctgcgcct gacggcgacg gccgctcccg
     2761 gctatgcctt cgaccactgg agcggggcgg ccgccggcga cgccaacccg ctggagatcg
     2821 acgcgacggg cgatttggcc gtcaccgctc atttcaggac ggttaatccg gccgcccccg
     2881 acctgtatct cagcccggag cagcccggca atgtgggcgg catcgccatc acgccgcagg
     2941 acatcatcaa gcgtgacggc gcgaccggcg cgtggagtct gtacttcgac ggttccgacg
     3001 tcggcatcac ccggcccatc gccgccttcg ctcgcttgca gaacggcgac ctgctgctcg
     3061 tgttccggtc gaggcaagtc atcgccgatg tcggcacttt caacgtcgct gatgtggttc
     3121 ggttcagccc gattacgaca ggcgaggtga ccgagggttc gttccactgg tacttcgacg
     3181 gctcggacgt tggtctgcaa ggcggtcggg agcagatcga cgcgcttgag gcgctgcccg
     3241 acggccgctt gctcatctcc acgaccggca cggccgccgt ccccaatgga gggaacgtgg
     3301 tgcgggcgca agatgaggat ctgctggcct tcaccccaac cacgctcggc gagacgaccg
     3361 ccggctcctg ggcgctctat ctggacggca cggccatccc cggcctggcg cgaaaagacg
     3421 tgagcggcgc ggccgtcgat tcggccaacg gcgacatcta catcaccatc gacggcaatt
     3481 accgcgtcgg cggcgtccgc ggcaacggca aggatattct cctgctgcat cccgtcggcg
     3541 acggctattc agtcgcgcgc tattggcacg ggccggacga cggcttccgg cccaacatcg
     3601 ggggtctgga gattgggggc gattgattga ggctggtctt tggaagtggg cagaaaccgg
     3661 cttcccggaa gaagcccggt ttgcgagggt gtgttgccgc cgtgggcctt atcccgcggc
     3721 ggccgcccaa acttcggtga cgggcactgc gaatccgggc agcaaagccg acggcgcttc
     3781 ggcctcatcg gtgaaccgac cctgttctat gtactcgccg ccctcaagac gcaggacggt
     3841 gatgatccgt tcgtcgggat cgatgatcca gtattcgctg atgccggcgg cggcgtattc
     3901 agcgcgcttg acgacccaat cacgtcgccg gtcctcgcgg ccgccgctca ccacctccat
     3961 cacgaggtcg gccccgtcgg ggtaatcttc cgcgaggcgt tcgggacgat cggccgaaag
     4021 aaagacgaca tccggctcgc gatagacgcg cggccgcagg cgcaccggca agggcgcaac
     4081 cagcacccag ccgagccggt gggccatgac atagtcgttg agaagacggt agaggtaaag
     4141 cacaatgaac tgatgcgcca tggtgggggt gggcaatacc tccacgaaac cgtccttgta
     4201 ttcaacttgc atgccgccgg gcagggcgag ataggcttcc tcgctccatt tgccctgggg
     4261 cgggtaaagg taggccaaat cccaaacagg ttcgccgccg ggataggggc gttgagttcg
     4321 ctttgttttg ggcaaaacag gtgtcggact catgagcgaa tgctaccacg cccggcggga
     4381 ggattcaacc gttgcagcgc cgcgcaaggc ttacaacgga taaaccagcg gcaaggtcaa
     4441 caggatcagg agcgtcaaca ggatcgtcag gggcaggccg accttgaagt aatcggcgaa
     4501 gcgatagccg ccgaggccca tcaccaggat gttgaccgga tgcccgaggg gggtcagaaa
     4561 ggccatcgaa gtggccagtg ccacacccaa tgacaggctt tgagggtcga tgcccgtttg
     4621 gcgcgccgcg ctgatggcca gcggcacgac gatggccgcc acggccgggc cgctgatgac
     4681 ctgcgccaac aacgtggtga ggatgaacag cgccgccagc agcgcgcgcg gcccataggg
     4741 cgcggcaaga gcgacgatct tcgcggcggc cagatcggcc aggcccgtgt tggtcagcgc
     4801 caggcccatc ggcagcatcc cggcgaccag gaccaccgct ttccactcga tcgcgccata
     4861 ggcctcgtcg gccgacagca cccccaggag aaccatcgcc aaagccccga ccagcatcat
     4921 ctcggccgtg gggatgatgt tggtagcgct gatcgccagc gtgatgacga cgatgaccac
     4981 cgccaacgcc agcttgtccg atcgaacggt cggctccgcc cccgaaggaa catcgagcag
     5041 gatgatatcc gggttttgct gcagcaggcg caagtgattg cgcgggcctt gcaggaacag
     5101 ggcatcgccg agctggaggg ggagcgtggt cattccgcgc cggatgggcc ggttgccgcg
     5161 ccagacggcc agcgccgaat agccgaattg ttcgcgaaag ctgacgctct ggagcgtctt
     5221 gccgaacagg ccggagcgcg gggcgagggc ggcttccacg acgatgatcc cctcggactc
     5281 caaatcgcgc tcctcaattc gcttgggggc cagaatctcc agatagggct gaatatcccg
     5341 ctcgcggaac tcttccaggc tgccttccag catcacgacg tccccgactt gcagcagcgt
     5401 gtcgggcggc ggggccaggc ggatttgccc gttgcgctcc agcgagagga cgttgacgtt
     5461 gaagcgctcg cggagcgaac tttgggccag cgatcggcct tcgagcatgg agccgggcgg
     5521 gatacgggcg cggaacaggc gctcgtcgag ttgatagact tcgacgagat cttcgcttcg
     5581 cgtagccaat gggcgattga gcacctcggc cgccggccga tggagcagca gccggcggcc
     5641 gaacgcggcc atataggcga tgccgacggc cgcaatcggc agccccagcg gcaggaaggt
     5701 gagcacgccg aagccgggca ggccttgctc gcgcagcagg ccgctggtga cgatgttggt
     5761 ggtggtgaac aaggtcgcca tgccacccag aatcgtggcg aaggccagcg gcatcaacac
     5821 ccgtgacggg ttggtgccct gtttgttggc cgccccggac acgccgggaa gcaggacggc
     5881 cgccgcggcg atgttgttca tgaacaggga caggaacgcc ccggcgatca tgaccaggat
     5941 caccagccgc ccctcggatc gaccggcgac ccggatcagc cagtcgccca ctttctcgcc
     6001 cgcgccggtc aggttaagcc cctgggccag gacgaagatg gtgacgatga tgacgacggc
     6061 cgaacggctg aagccggaga acgcttcctg cggggtgagg atgccggtcg cgcccagact
     6121 gacgaggacg agcagggcca ccacgtcggc gcgcaggcgg ttggtgagaa agaggacgat
     6181 ggccgccgtg aggatgatca gtgtgatgac tgcgtcggag gaaagcgcca tgtcgaaccc
     6241 ggccgattat ttcggctgcc gatcgttaaa aatcggtcga tttcaacgcc gccatcagcg
     6301 acgccacatc gtcggcgatc agcttcagca gccgcagcag ctccttgcgc tcagcggccg
     6361 tcaggctgtg gcggtcgacg cgggccagat ggccgcgcac ccagtcgagc cgctcgatgc
     6421 tggtcggcgc gccgacgcgg gcggggcgca gcgggtcgga tgtgaacgtc gcgccgcgcg
     6481 gttccagctc gttgggcagc ggagcggggg agccgggggg cgggggagca ggggtgattg
     6541 gatcggcttc ctggggtgcg agttcatcat cggccgtttg ctccagcagg cgggcggcgg
     6601 cggcgacggt catctccggc gcttcgcgca gcaagcgggc tagctggcgc acctcgacgg
     6661 cgttaagcgc accatccatc cgcgcccgat ggagcgcccg ctgctggacg gccgtcagtc
     6721 cctgatagac gcgcgtgtcc cgctcgctca atgatccgtc gcgcacggcg tccttgatct
     6781 cgtcgggcag gtcgagtaac tggatgagct ggtggatgcg ctggcggctg tagccgaggc
     6841 ggccgccgac cttggcccag gtgatcttgc cgccccaggg ttcgccggcc tgttgctcgg
     6901 ccgcgtccag ctcttcctgc atcagcttgc gcaggcgcag cagggcggcg gcgcggtcga
     6961 cgtcattgag gtcttcgcgc tggatgttct ccaccacctg gcgcacgaag cgggtcacgt
     7021 cgtcgtagtc gcggcggcgc accacggcgg gcacggtggc cagcccggcc agctgcgacg
     7081 cccgccagcg tcgctcgccg tggacgatca gatagcggcc ggggctgctc tgggtcacct
     7141 cgatcggctg gatgacgccg tcctggcgga tgctctcggc cagctcatgc aggctgtcgt
     7201 cgttgaaggt gctgcgcggc tggtcggggt cggggtcgat ggcatcgacg cgcacggcca
     7261 acagttgcag cccggcggcc tgctccgcgt ccccctcggt ggcgaacagc tgctcaaggt
     7321 cgccgccacg cggctggatg ggctgggaga ggttgatttt ggtgcgtttc ttg
//
LOCUS       DAOMQG010000147         7316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4362_length_7316_cov_9.588624, whole genome shotgun sequence.
ACCESSION   DAOMQG010000147 DAOMQG010000000
VERSION     DAOMQG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4362_length_7316_cov_9.588624"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1470)
                     /gene="radA"
                     /locus_tag="PLC06_09280"
     CDS             complement(97..1470)
                     /gene="radA"
                     /locus_tag="PLC06_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRO90676.1"
                     /translation="MAKRQTQYVCQSCGRTTPAYMGRCPRCGEFDTMVEQLIEPETPV
                     KGRKSGGLTSSRPVRLHEVEADGFDRLALPVQEFSRVLGGGLVPGSLILVGGDPGIGK
                     STLLLEIAGLTANLHGPVLYVSGEESARQIKMRAERLRMDANDLFLVTETSLHAILDH
                     INTVRPVMTIVDSIQTTYDDNLNSSAGSVSQVRESAARLQEVAKQSGVIIVLVGHVTK
                     EGAIAGPRVLEHIVDTVLYLEGDPFHRYRLLRSVKNRFGATSEVGVFEMVEQGMIEVL
                     NPSEAFLAERQANAPGSAIAVTLEGTRPLLVEVQALASATTFSNPRRTPNGIDMNRLL
                     LLTAVLTRRVHVPLHDKDVFVNVIGGLQISEPAADLGVALAIASSVKDRAVHADMAFF
                     GEVGLSGELRAVSQLEPRLKEAAKLGFKRCVVPRTRQLKSTAVPQGLQIIECRTLQEA
                     VEVSLVK"
     gene            1610..1912
                     /locus_tag="PLC06_09285"
     CDS             1610..1912
                     /locus_tag="PLC06_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90677.1"
                     /translation="MINWYSIIANSFWLLGLAVVLAGLSYYYWLAVQTGRSLGDMFGA
                     SSFQRVIILGALLIGVGLALTAGSLWQMLPAIVLIGVSVVALVTSFRTRSDTPPTR"
     gene            1927..2343
                     /locus_tag="PLC06_09290"
     CDS             1927..2343
                     /locus_tag="PLC06_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90678.1"
                     /translation="MNRTRLAAFSLAALILILALASQPALAANGHQEGYPPPLLPTPT
                     LAVPVEMPGEPPVEPYPSGGTDFNGQPPVPIEIGSGAASGEENLMSEPPNTMSEEGST
                     PDRGIVFLWLGFIATSLIFLTSVFGSIILFTRRNES"
     gene            2445..3500
                     /locus_tag="PLC06_09295"
     CDS             2445..3500
                     /locus_tag="PLC06_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRO90679.1"
                     /translation="METDGQSRASWQRYLPWLNLGLTLALIIAGLWYLSTRLDPAVLL
                     DVLLAASPGYVSLSVLIMLLTMLIKALRWQLMFPTERPPVRLAPSFWAISLGQYVNLV
                     VPFLRLGEVARLYALNQEAGTSPGRALGSLVIEKTLDLIFFGLTILFVLPFVILPDFM
                     GDPGFMLWLLPLVLLAVLYILAYQTDLVIRLWRRIVQPLPARLRELLLRLAVSGLEGL
                     GSLRDRRLTLYMLLLSVAIALLSIALPYALFPAMSLPLGFLDAALVHIVVTIAIIPPS
                     TPAKIGVLNGAAAVTLWQLGISDEATIAGYTILLYLVVVVPQIILGLLAASRTKWRWQ
                     TTLPGDPATTGANPRLP"
     gene            3497..4507
                     /locus_tag="PLC06_09300"
     CDS             3497..4507
                     /locus_tag="PLC06_09300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO90680.1"
                     /translation="MSRPHCSVIVPAYNAERTIGACVAALCAQQFDQPHEIIVIDDGS
                     TDGTAAAARAAGATVITTPNGRPAAARNAGIRAASGEIICCTDADCIPHPDWLRQITF
                     PLADSDIVACKGSYATRQTELVARFVQLEYEDKYDLLRTEKYIDFIDTYSAAYRRETL
                     LECGGFDERFDYLEDQELSFRLAAKGYKMVFQPEAIVDHLHSATPGAYLRKKQIIGYW
                     KAQVVRRFPKRAVKDSHTPQVMKVQMALSVLLVGLTLLGLAGAALFPAYRETSGWFSL
                     LPTAIVALIFVATTLPFVRKAWPKDRAVAFVSPVLLLGRALALSAGYIHGILRPRRLG
                     ED"
     gene            4791..6242
                     /locus_tag="PLC06_09305"
     CDS             4791..6242
                     /locus_tag="PLC06_09305"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRO90681.1"
                     /translation="MSQTIDSNTAIAPLTGSKLLPLASGFIALGVYLLTIAPDLTWAN
                     AALDGVEFVTASATLGIPHPPGYPTYVILGKAFSWLPLGSIAFRYNLFSAVAVAATAG
                     LLVSIIVSLHKQIRPTTAMVAALLFAFSPLVWSQAVVTEIYGLNLLFVAILLLVWIRR
                     GPAGWAGFWLGLAITTHLTSIFFLPALMLAAGRRFWRPLVGMAVGLLPWLLPPILAAD
                     NSPIIWGLPTNIQGWWWLISGNLYTANFQPSIDIDHLAHILQALAFGPVGMLMAVNAN
                     ESAIQAGNPAGIDHQRMIRLLGFTAIIYALFAVFYRTSDTAVNLLPALMILALLIAPI
                     LDRLGKAALLLPLALAVLTFPSRNLSGDYEVRLNAGQLLTEAPENALLLTPGNRTIFT
                     ILYFQHVEKVRPDIRVVDANLFAFDWYRDRLRAQHPELYVPKGDDLALFRQVNKETRP
                     FCLANLVPEAGMLSIDHQRGEARSLASPQLDCE"
     gene            6251..>7316
                     /locus_tag="PLC06_09310"
     CDS             6251..>7316
                     /locus_tag="PLC06_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HRO90682.1"
                     /translation="MIPDNRYSSSPPAAEQPSPPNSTARIARLLVLAGILILLLWLGL
                     KAWRVYQSTRSLLTIEPEVRALMAGGIKQIDPDAAEALVLRARADIAVLYDELAIVRP
                     IAPYLGWVPRLGPTLVASPYLLEMADAGSEAGALAVSSLKPALAVFQREDFGMASLGE
                     LLPIISNAAPELESASAAITRYVAARRGLSAAVPTDTLPWRVRQLLEVSDKWQPIGED
                     GLRLLPQFPSLMGQDGPRRYLILAQNEDEMRPTGGFITGAGVITVQNGRITSLEFRDA
                     NNVDNWRDKPYAFPPQPFYDFMRLELFGFRDANFWPEFPVSAQKAMQLYEYGLDAPPL
                     DGVIAIDQEFLRVLVDATGPV"
BASE COUNT         1394 a         2257 c         2011 g         1654 t
ORIGIN      
        1 cggggaatgg tcgtgcttta ccattgccgg gcaacgaatt cgctattata gcctcagggg
       61 agaatatttc aaggggttgg tcaggcagaa accgagctat ttcaccagag agacttccac
      121 cgcctcctgc agcgtgcgac attcaatgat ctgcagcccc tgtggcacgg cagtcgattt
      181 taattgacgc gttcgcggca ccacgcagcg tttgaagccc agcttggccg cttctttcaa
      241 ccggggttcg agttggctga cggcccgtaa ttcgccgctc aaccccacct cgccgaagaa
      301 cgccatatcc gcatggacag ctcgatcctt gacgctgctg gcgatggcca gggcaacccc
      361 cagatcggcc gccggttcag agatctgcaa gccgccgatg acgttgacga acacgtcttt
      421 atcgtgaagc ggtacatgca cgcgacgagt caggacggcg gtcaatagca acagccgatt
      481 catgtcgata ccgttcggcg tgcgacgagg attactgaac gttgtggcac tggccagcgc
      541 ctgcacctcc accagaagcg gccgcgtgcc ctccagcgtc acggcaatag ccgagccggg
      601 ggcgttagct tgccgttccg ccagaaacgc ctccgacggg ttaagcactt cgatcatccc
      661 ctgctcgacc atctcgaaca caccgacctc actcgtcgcg ccaaagcggt tcttcacgct
      721 gcgcaaaaga cgatagcgat gaaacggatc gccttccaga tagagcactg tgtcgacgat
      781 atgctccagc acgcgcggcc cagcgatggc gccctctttg gtcacgtggc caacgaggac
      841 gatgatgacg cccgattgct tggccacttc ctgtagccgg gcggccgact ctcgcacctg
      901 gctgacactg ccggccgatg agttcagatt gtcgtcatag gttgtctgga tgctgtcgac
      961 gatcgtcatc accggtcgga ccgtgttgat gtgatccagg atggcgtgga ggctggtctc
     1021 ggtgacgaga aacagatcgt tggcatccat gcgcaggcgc tcggcgcgca tcttgatctg
     1081 ccgcgcgctt tcttcgccgg agacatagag caccggtcca tgcaggttgg cggtcagccc
     1141 ggcgatctcc agcagcaacg ttgatttgcc gatcccggga tcgccaccca ccaggatgag
     1201 cgagccgggg accagccccc cgccgagcac tcgcgagaat tcctgaacgg gcaaggccag
     1261 ccggtcgaag ccatccgctt caacttcatg caaacgaacc ggacgggatg acgtcagtcc
     1321 acccgacttc cggcccttga ccggcgtttc cggttcgatc aattgctcga ccattgtgtc
     1381 gaattcgcca caacggggac agcgtcccat ataggccggt gtcgtgcggc cgcaactctg
     1441 gcatacgtat tgcgtttgtc ttttcgccat gtccctagta tacccttatc cggcaagatc
     1501 aacggatcaa tcgacacaca aatcgctcaa ttgttctgtt attcacagcc atttttcatg
     1561 cgatcaaaac ggcaactaca acgttggagt gatataatgc cggagactta tgattaactg
     1621 gtattccatc atcgcaaaca gcttctggct gctgggcctg gcggtcgtcc tggccgggtt
     1681 aagttactac tattggctcg ccgttcagac cggtcgctcg ctcggggata tgtttggggc
     1741 atcgtccttt caacgcgtga tcattcttgg ggcattactg ataggcgtcg gcctggcgct
     1801 gacggccggc agcttgtggc agatgctccc cgccatagtc ctgattggag tcagtgtggt
     1861 agccctcgtg acctcattcc gcacacgttc cgatacaccg ccaactcggt aaggcataat
     1921 gtctctatga acagaacacg actcgccgcc ttttcgcttg cggctcttat cctgattctt
     1981 gctcttgcat cacagccggc attggccgcc aacggccatc aggaaggata tccaccaccg
     2041 ctgttaccca cgcctacgct cgcagtcccg gtggaaatgc ctggcgagcc tcctgtcgag
     2101 ccttatccct ccggcggcac cgacttcaac gggcaaccgc ctgtgccaat cgagatcggc
     2161 agcggcgcgg catcaggaga agagaatctg atgagcgagc ctcccaatac aatgtccgag
     2221 gaggggtcaa cccccgaccg cggcatcgtg ttcttatggt tgggctttat cgcgacgtcg
     2281 ctcattttcc tgacgagcgt cttcggttcg atcatcctgt tcacgcgacg gaacgaatcc
     2341 tgacaaaagg tttccacggc cggcatgaag aaaagagttc ccccgtacac ctgcgcaaac
     2401 gcgatcgatg ggcacgaaac caccggccgc agatgagaag catattggaa acagacggtc
     2461 aatccagagc gtcatggcag cgatatttgc cctggttgaa cctggggctg actctagccc
     2521 tgatcatcgc cgggctatgg tatttgtcca cacgcctgga tccggccgtg ctcctggatg
     2581 tgttgctggc ggcatcgccg ggttatgtga gcctgtcagt gctgattatg ctgctcacga
     2641 tgctgattaa ggcattgcgc tggcaattga tgtttccgac tgagcgtcct cctgttcgcc
     2701 ttgccccatc cttctgggcg atttcattgg gtcaatacgt aaatttagtc gtccctttcc
     2761 tccgcttggg cgaggtggcc cgtctctatg ctctcaatca ggaagcaggc accagcccgg
     2821 gaagagcgct tggctcattg gtcatcgaga aaacgctgga cctgatattc tttggtctga
     2881 cgatcctgtt cgttctgccc ttcgttatcc tgcccgactt tatgggcgac cccggcttca
     2941 tgctctggtt gctgccgttg gtcttgctcg cggttctcta tatcctggcc tatcagaccg
     3001 acctggtcat ccgcctgtgg cggcgtatcg ttcagccatt accggcgcgc ttgcgcgaat
     3061 tgctgctccg tctggctgtc agcgggctgg aagggctggg gtctctccgc gatcggcgat
     3121 tgaccttata tatgctgctc ctttcggtgg caatcgctct tctgtccatc gctctcccct
     3181 atgcactgtt cccggccatg tcgcttccgc tcggctttct tgacgcggcg ttggttcaca
     3241 ttgtcgttac gattgccatc atcccaccaa gcacaccggc caagatcggc gtactcaacg
     3301 gcgcggccgc cgttaccctc tggcaactcg gcatttccga tgaggcaact atagccggct
     3361 acaccatcct gctctatctg gtcgtggtcg tgccacaaat catccttggg ttgctggctg
     3421 cctcgcggac caagtggcgg tggcaaacga ccttgccggg cgaccccgca accaccggcg
     3481 caaatcccag actgccatga gccggccgca ttgctcagtc atcgtccccg cctataacgc
     3541 cgagcgcact atcggcgcgt gcgtggccgc cttgtgcgcc caacaattcg accagcctca
     3601 cgagatcatc gtcatcgatg acggctccac cgacgggacc gcggccgcgg cccgagcagc
     3661 cggggcaacg gtcatcacta ccccaaacgg ccgacccgcg gccgcgcgca atgccggtat
     3721 ccgggccgcg agcggagaaa ttatctgctg caccgacgcc gattgcatcc cccatcccga
     3781 ttggctgcgc cagatcacct tccccttggc ggattccgat atagtcgcct gcaaaggcag
     3841 ttatgcaacc cgccaaacgg aactggtcgc ccggttcgtc cagctggagt acgaggacaa
     3901 gtacgatctg ttgcgcacgg agaaatacat cgacttcatc gacacctatt cggccgccta
     3961 ccggcgcgag actcttctgg agtgcggcgg gttcgatgag cgttttgatt atctggagga
     4021 tcaggaactt tccttcagat tggcggcaaa gggttacaag atggtattcc agcccgaggc
     4081 catcgtcgac cacctccata gcgccacgcc aggcgcttac ctccgcaaaa aacagattat
     4141 cggctattgg aaggctcagg tcgtccgccg cttccccaag cgagcagtca aggattccca
     4201 taccccgcaa gtgatgaagg tacagatggc cttgtcggtg ctgctcgtcg ggctaacgct
     4261 gctcggcctg gccggcgcgg cattatttcc tgcatatcgg gaaacttcgg gctggttcag
     4321 cctgctcccg acggcaatcg ttgcgctcat tttcgtggca acgacattac ccttcgttcg
     4381 taaggcatgg cccaaggacc gtgccgtggc gttcgtctcc cctgtgctgc ttctcggccg
     4441 agcgctggcc ctgagcgcag gttacattca tggaatatta cggccacggc gtttgggaga
     4501 ggactgagca ggggaataat cttgcgcgcg aacaagccga ttccaggtat tttccgactt
     4561 cttgccggcc gactattcac gatcattgca tggcctcctc ctgctctatt cattaaactg
     4621 gactcggagg gcttgacgcc acccggacag gagtacgcca gacgcactgg caagcataac
     4681 catgttcaag tttcacacca tgcatcggaa cgtggtcgcc gaacgacata atcccgtcac
     4741 ctcaagaaga ttatgggagc ttattgttcg gcaaggggcg tcttaacgtc atgagccaaa
     4801 ccattgattc caatacggcc attgctccat tgaccggatc gaaactcctg ccccttgcct
     4861 caggtttcat cgctctggga gtgtatttgt tgaccatcgc ccccgacctg acgtgggcca
     4921 atgcagctct tgacggtgtg gagttcgtca ccgcctcggc cacgctcggc atcccccatc
     4981 cgccaggcta cccaacctac gtcatcctgg gcaaagcatt cagttggctt ccgctcggga
     5041 gcatagcatt ccgctacaac ctgttttcgg ccgtggccgt ggccgctaca gccgggcttc
     5101 tcgtttcgat cattgttagc ttgcacaagc agattcgccc aactaccgcg atggttgcgg
     5161 cgctcctgtt cgccttttct ccgctcgtct ggagccaggc tgtcgtcacc gagatctatg
     5221 ggctgaacct gttgtttgtg gcaatcttgc tgcttgtctg gattcgccgc ggccccgccg
     5281 gatgggccgg attctggctg ggtctggcga taacgaccca cctcacctcg atattctttt
     5341 tgccggcgtt gatgctcgcc gccggccgcc gattttggcg gcccctggtc gggatggcgg
     5401 tcgggttact cccctggctt ttgcccccga ttctggccgc cgacaatagc cccattatct
     5461 gggggctacc aacaaacatt cagggctggt ggtggttgat cagcggcaat ctctacactg
     5521 ccaattttca gccatccatt gatatcgatc atctcgcgca tattttgcaa gctctggcgt
     5581 tcggcccggt tgggatgttg atggccgtca atgccaacga atcggccatt caagccggga
     5641 atcctgcggg catcgatcat caacgaatga ttcgtttgct agggtttaca gcgatcatct
     5701 acgctctgtt tgccgttttt tatcggacgt ctgacacggc tgtgaatctg ctcccggctt
     5761 tgatgatcct cgcgctatta atcgcgccaa tcctggatcg cctgggcaaa gctgctctgt
     5821 tattacccct cgctctggcc gtgttgacat tcccatcccg gaacttaagc ggagattatg
     5881 aggtacggct gaacgccggg caattgctca cagaggctcc ggagaacgca ctcttgctaa
     5941 ccccgggaaa tcgcactatc tttaccatct tgtattttca acacgtcgaa aaagttcgcc
     6001 ctgatatcag ggtcgtggac gccaacctgt tcgccttcga ctggtatcga gaccggctga
     6061 gagcgcagca tcctgaacta tacgtaccga agggggacga tctggcctta ttccgacagg
     6121 tgaacaaaga aacacggccg ttttgtctgg ccaacctcgt tcccgaagcg ggcatgttgt
     6181 ccattgatca ccaacgaggc gaagcgagat ctctcgcctc gcctcaactt gattgcgagt
     6241 gattgcgaac atgattccag acaatcgcta ttcatcttct ccacctgcgg ccgaacagcc
     6301 atcgccgccc aactccacag cccgaatcgc acgcttgctc gttctggcgg gtatcctgat
     6361 tctgctgcta tggcttggcc tcaaggcatg gcgggtctat cagtcgacac gatccttgct
     6421 gaccatcgaa cctgaggtac gcgcgctcat ggccgggggt ataaaacaga tcgatccgga
     6481 cgcggccgag gcgcttgtgc tcagggcgcg agccgatatc gccgtgctct acgatgaact
     6541 ggccatcgtc cgccccatcg cgccttatct aggttgggtg ccgcgtctgg gcccaacgct
     6601 tgtggcatca ccctacctat tggagatggc ggacgccggt tcggaagcag gtgcattggc
     6661 cgtcagcagc ctcaagccgg ctctggccgt cttccagagg gaagatttcg gcatggccag
     6721 cctgggggag ttattgccga tcatttccaa tgccgcgccg gagcttgaga gtgcctcagc
     6781 cgcaataact cggtacgtgg ctgcccgacg aggactatcg gccgctgttc cgaccgacac
     6841 cctcccctgg cgcgttcgcc aactgttgga agtctccgat aaatggcagc ccatcggcga
     6901 agatggattg cgcctgctcc cgcaatttcc gtcgttgatg ggccaggacg gtccgcgacg
     6961 ttatctgatc ctggcccaga acgaagacga gatgcgaccg accggggggt tcatcaccgg
     7021 ggcaggcgtc ataaccgtcc agaacggccg aatcaccagc cttgagtttc gcgatgccaa
     7081 caacgtggat aattggcgcg acaagcccta cgcattcccg ccccaaccct tctatgactt
     7141 tatgcgtctg gagcttttcg gcttccgcga cgcgaacttt tggccagagt tcccggtgtc
     7201 ggcgcaaaag gcgatgcaac tctacgagta tgggctcgac gcgccgcctc ttgatggcgt
     7261 gattgccatc gatcaggagt ttcttagagt gctggttgat gccaccggcc cggtac
//
LOCUS       DAOMQG010000148         7284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4382_length_7284_cov_10.845068, whole genome shotgun sequence.
ACCESSION   DAOMQG010000148 DAOMQG010000000
VERSION     DAOMQG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4382_length_7284_cov_10.845068"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLC06_09315"
     CDS             <1..233
                     /locus_tag="PLC06_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90683.1"
                     /translation="NPRSERLDAYKITVGNGLSSRKNHIILSAARRDYLPKLVMLSYN
                     MTNSDDKLCHSSSRQVTNDNRKRPVQHLYWLV"
     gene            318..1790
                     /locus_tag="PLC06_09320"
     CDS             318..1790
                     /locus_tag="PLC06_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRO90684.1"
                     /translation="MIDLSIHEAPLERAVQRAREQNIIIPTFAQQRDPSLIPGAIKRR
                     LSEVGLWDLNPVNLFRITWRNEPVVQGGGFGDVNYMELPKEITGVDARVVVIVGKWFP
                     TGAHKVGAAYGCLVPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSRLLGCESIAILPE
                     GMSRERFEWLEGVADEIIRTPGTESNVKEIFDKCWELRRSGQDLVIFNQFDEMGNYLW
                     HYSVTGPAMHEILKRVMGPGDSYRGMASATGSGGTIASGDYLKEQYPHSKICASEALQ
                     CPTLLNNGFGAHRIEGIGDKHIPWVHNVKNTDMVVAIDDEATIQIMRLFNEEIGNEYL
                     AMQGVSPEIIEQLPLLGISGIGNVLSAIKMAKYYEMGPNDVVMTIATDSMEMYGSRVV
                     ETREEDGPLTMLDAAGIYHRYLLGERTDNLIELTYAERKRIHNLKYYTWIEQQGKTSE
                     ELNAQWYDPTYWTSLHGMDAEIDKLIEQFNERVGLLGEMN"
     gene            complement(1884..3806)
                     /locus_tag="PLC06_09325"
     CDS             complement(1884..3806)
                     /locus_tag="PLC06_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRO90685.1"
                     /translation="MYRRTVRLFVILFSMLLLTACEFSASTANVKSATLARDANGTQP
                     TTTFEPSDTFYLIVDLANAPDDTTVKAIWYAADVGSAAAPNTLIDQASLTSGSSVLTF
                     NLSPESAWVPGTYKVELYLNDELNQTLNFSVNGEVVKAQPTSAPAAADPTAAPAAGSD
                     LSTTLEGVRSATIRIQAEGSFIDPEFGEMTNAAGQGTGFIIDPSGIAVTNNHVVTGSA
                     FLQVYVEGEDEPRNARILGVSECSDLAVIDIDGDGFPALQWREGDIPAGLEIYVAGFP
                     FFGNEEFTLTRGIVSKASADGETNWASVDSVIEIDATINPGNSGGPLVDGDGRVVGVN
                     YASSSGTDQYFTISRDEALPVIEQLRAGKDVNSIGINGQAVSDGESIFGIWASSVESG
                     SPADKAGIKAGDILTKMEGLVLATDGTMSAYCDILRGHNMTDPMSVEVLRFADGTLLT
                     GQLNGDPLEIVGTFGTDTGGSSGDTSGGATGGSYSEYVSVRDDSGMLQMSVPVEWSDI
                     DGTPWTFEDEEAGPSLNAAVDVDGFYDNWTTPGAFLGASTSLRATYDPNSFLDQFDLS
                     DSCTYDGRSDYSDELYTGAYDSYSNCGGTGTSFLVLVVEPESQGVLIILQVQMVTAAD
                     QEALQRILESFIYFGL"
     gene            4134..4856
                     /gene="minC"
                     /locus_tag="PLC06_09330"
     CDS             4134..4856
                     /gene="minC"
                     /locus_tag="PLC06_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRO90686.1"
                     /translation="MARQIDIKGIRDGLLIRVNDHPDFAVYEPLRRELEQKRGFLHDS
                     RITVELGRRAMNGEQLAGLRDLFADHDMELWAIIADDGETRNLAREMGLATRLSGSAT
                     DLDGNALSHAVAEHVPNGDGAKAAPSSPVAGNALLLRETVRSGRLVWHEGHVVVLGDV
                     NPGAQIVATGNVIVWGRLRGLVHAGASGDATAIICALDLSPTQLRIADHIAVAPNDSH
                     AASIPEQATIREGQIVAEPWRH"
     gene            5005..5814
                     /gene="minD"
                     /locus_tag="PLC06_09335"
     CDS             5005..5814
                     /gene="minD"
                     /locus_tag="PLC06_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRO90687.1"
                     /translation="MSAQVLTVTSGKGGVGKTTVTANLASALAMLGKRVTAIDADIGL
                     RNLDVVMGLENRIVYDLVDVVEGRCRLRQAMIKDKRQPELYLIPAAQTRDKNAVSPSD
                     MVLICDQLREEMDYIVIDSPAGIERGFRNAVAPADKVLIVTNPEVSAVRDADRIIGLI
                     EAEEKGPGQLILNRVKPAMVSRGDMLSVDDVLDILAVNLLGVIPEDERILVASNQGMP
                     LVMHGVNGASAGEAFRNVARRLDGEDVPFLDLTPKQTLMGRLRALMGSNSN"
     gene            5881..6159
                     /gene="minE"
                     /locus_tag="PLC06_09340"
     CDS             5881..6159
                     /gene="minE"
                     /locus_tag="PLC06_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRO90688.1"
                     /translation="MNWLDRLLGKNETSGQVAKQRLQMVLIHDRADLPPGMLELIKDD
                     IIQVIARHMAIDTDKVVVHLEQDTQENRLVAEIPLLAGRTRRNGASSR"
     gene            6254..>7284
                     /locus_tag="PLC06_09345"
     CDS             6254..>7284
                     /locus_tag="PLC06_09345"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRO90689.1"
                     /translation="MLSRTSVWRFFDVWLLAAVLLLTGYGILMIRSAITGAPAFAGYP
                     QQQLIWAILGLVILLVTAAIDYRVLTSAHWWIYAGLVGLLVLVIVTGQVNNDARRWIQ
                     ITPSFQIQPSEFGRIFISITFAQFLANRRQLIARFDNTIVTLAYFAIPVGLIFIQPNL
                     GMTILFMFLWFIMIWVAGLPLRHFLMLGMIGLAVGAAVFPFLADYQKGRITTFLNPEE
                     ASADDVFNIDQAEISIGSGGLLGKGYLEGTQSQLGFLRAQHTDFIFSVVTEEMGLLFG
                     SLVVLGLMGFILLRILRAASLSPDPAGKLICVGIAAILFFQTAVNVGMNVRVLPVTGL
                     TLPFVSYGG"
BASE COUNT         1459 a         2157 c         2215 g         1453 t
ORIGIN      
        1 ggaaccctcg atcggaacgc ctcgatgcct ataaaataac agtcggtaac ggtttgtcaa
       61 gccgcaaaaa ccacatcatt ttatcggctg cccgccgcga ctatttgccc aaattggtca
      121 tgttgtccta caatatgacc aattctgatg acaaattatg ccattcgtca tctcgacaag
      181 tgacgaatga caatcgcaag cgtccggttc aacacttata ctggttggtg tgagcaagac
      241 tggtcccatt tgcccgaatt catcccggtt tgccacggga tcccacaaaa accttgtaac
      301 cgtctgagga gaataccatg atcgatctgt ccattcatga agccccactc gaacgcgccg
      361 ttcaacgcgc ccgcgagcag aacatcatca ttcccacgtt cgcccaacag cgcgacccgt
      421 cgctcattcc cggcgccatc aagcggcggc tgagcgaggt gggattatgg gacctgaatc
      481 ccgtcaacct gtttcgtatc acctggcgca acgagccggt tgtccagggc ggtggcttcg
      541 gcgacgtgaa ctacatggaa ttgccgaaag agatcaccgg cgtcgatgcc cgcgtcgtcg
      601 tcatcgtcgg caaatggttc cccaccggcg cgcacaaggt cggcgcggcc tatggctgcc
      661 tcgtgccgcg gctggtgacc ggccagttcg acccgaccac gcagaaggcc gtctggccca
      721 gcaccggcaa ctactgccgc ggcggggctt atgattcccg cctgcttggc tgcgaatcga
      781 tcgccattct gcctgaaggg atgagccgcg agcgcttcga gtggctggaa ggcgtggccg
      841 acgagatcat ccgcaccccc ggcacggaga gcaacgtcaa ggagatcttc gacaaatgct
      901 gggagctgcg ccgctcgggg caagacctgg tcatcttcaa ccagttcgac gagatgggca
      961 actacctgtg gcactatagc gtcaccggcc cggcgatgca cgagattctc aagagggtta
     1021 tggggccggg cgattcgtat cgcggcatgg cctcggccac cggttcgggc gggacgatcg
     1081 ccagcggcga ctacctgaag gagcaatacc cccacagcaa aatctgcgcc agcgaggcgc
     1141 tgcaatgccc gacgctgctc aacaacggct ttggcgcgca ccgcatcgag ggcatcggcg
     1201 acaagcacat cccgtgggtc cacaacgtca agaacaccga catggttgtg gccattgacg
     1261 acgaggcgac catccaaatc atgcgcctgt tcaacgagga gatcggcaat gagtatctgg
     1321 ccatgcaggg cgtgtcgccg gagatcattg aacaactgcc gctgctgggc atttccggca
     1381 tcggcaatgt gttgtcggcg atcaagatgg ccaagtatta cgagatgggg ccgaacgacg
     1441 tggtcatgac catcgccacc gactcgatgg agatgtacgg cagccgcgtg gtcgagacgc
     1501 gcgaggaaga cggcccgctg accatgctgg acgcggccgg catctaccac cgctatttgc
     1561 tgggcgagcg caccgacaac ctgatcgagt tgacctacgc cgagcgcaag cgcatccaca
     1621 acctgaagta ctacacctgg attgagcagc agggcaagac gtcggaggag ctgaacgcgc
     1681 aatggtatga tccgacctac tggaccagcc tgcacgggat ggacgcggag atcgacaagc
     1741 tgattgagca gtttaatgag cgggtggggt tgttggggga aatgaattag tttgggttcg
     1801 ggtgtttggc acttgaacga cgaaatataa aagaaaaggc cccgtgcatg gggccttttc
     1861 ttttttgttc cgcagatcct ttcttacagc ccgaaataga tgaagctctc cagtatccgc
     1921 tggagcgctt cctggtcggc cgcggtcacc atttgcacct ggagaatgat caatactcct
     1981 tgtgactccg gttccaccac cagaacgagg aagctggtgc ccgtgccacc gcaattggag
     2041 taggaatcat acgcgccggt atacagttcg tcgctgtagt cggagcggcc gtcataggtg
     2101 caactgtcgc tcagatcgaa ctgatcgagg aagctgtttg ggtcataggt ggcccgcagg
     2161 gacgtcgacg cgcccagaaa tgcgcccggt gtcgtccagt tatcataaaa gccatcgaca
     2221 tcgaccgctg cattgagaga gggaccggct tcttcatctt caaaggtcca gggtgtgccg
     2281 tcgatgtcgc tccattccac gggaaccgac atttgcagca tccccgaatc gtcgcggacg
     2341 gagacatact cgctgtagga gccgccggtc gcgccgcccg acgtatctcc ggacgaaccg
     2401 ccggtgtccg tgccgaaagt gccgacgatc tccagcggat cgccgttgag ctggccggtc
     2461 agcaacgtgc cgtcggcaaa gcgcagcacc tcaaccgaca tggggtcggt catgttgtgg
     2521 ccgcgcagga tatcgcaata ggccgacatc gtgccgtcag tggccagcac cagcccttcc
     2581 attttggtca ggatgtcgcc cgccttgatg cctgccttgt cggccgggga gccggattcg
     2641 accgaagagg cccagatacc gaagatactc tccccgtcgc tcacggcctg gccgttgatg
     2701 ccgatggagt tcacatcttt gcccgcgcgc aattgctcga tgaccggcag cgcctcatca
     2761 cgagagatgg tgaaatactg gtcggtgccg gagctggagg cgtagttgac gcccaccacg
     2821 cggccgtcac cgtccaccag cgggccgccg gagttgccgg gattgatcgt cgcatcgatt
     2881 tcgatgacgc tatccaccga ggcccaattg gtctcgccat cggcgctggc cttcgagacg
     2941 atgccacggg tcagagtgaa ttcctcgttg ccgaagaacg ggaatccggc tacatagatc
     3001 tccagcccgg cggggatgtc gccctcgcgc cactggagtg ccgggaagcc gtcgccgtcg
     3061 atgtcgatga cggccaggtc ggagcattcc gacacgccca ggatgcgggc gttgcgcggc
     3121 tcatcttccc cttccacata gacctgtaga aaggcagagc cggtgacgac gtggttgttg
     3181 gtgacggcga tgcccgacgg gtcgatgatg aaaccggtcc cctggccggc ggcgttggtc
     3241 atctcgccga actcagggtc gatgaagctg ccctcggcct gaatgcggat tgtggcgctg
     3301 cgcacccctt ccagcgtggt tgacaggtcg gacccggcgg ccggcgcggc cgtgggatcg
     3361 gcggcggccg gggccgacgt tggctgggcc ttcaccactt cgccgttgac gctgaagttc
     3421 agcgtctggt tcagttcgtc gttgaggtat agctcgacct tgtaggttcc cggcacccag
     3481 gcgctttcgg gcgagaggtt gaatgtgagc acggagctgc cggaagtgag gctcgcctgg
     3541 tcgatcaagg tgttgggcgc ggccgccgag cctacatcgg ccgcgtacca gatcgctttg
     3601 accgtggtgt cgtcgggtgc gttggccaaa tcgacgatca gataaaacgt atcagatggt
     3661 tcaaaagtcg tggtgggctg ggtgccgttg gcatcacggg ccagcgtggc gctcttgacg
     3721 ttggctgtgc tggcgctgaa ttcacaggcg gtcaacaaca gcatggaaaa caagatgacg
     3781 aacaggcgaa cggtgcggcg atacatcttc tttctccttg aaccaactct attatgtttt
     3841 aatgtgcgcg acccaagcga ccgtagctcc ctggtcaccg ccacgctctt tgagaatcat
     3901 aaatgatagc acctataggg taatgtgaaa aatgcgtaaa atttaatacg tcctgaacta
     3961 ttttactgcc tgatggtcgg ggaaattcgc ttgcactgcg attgccctcg ggcgcgattg
     4021 ctctttcttc acttgctatt cggccgatgt tgttgtatta tttcaacaat tcggctcaat
     4081 gatttccggc aacaccgcca tatccgggct aatcggggtc gattcgggca caggtggcac
     4141 gacagataga catcaaaggg attcgcgacg gcttgttgat ccgggttaat gaccacccgg
     4201 actttgccgt ttacgaacca ttgcgacgtg aactggaaca aaagcgcggc tttctccatg
     4261 acagccgaat caccgtggag ttgggtcgtc gggccatgaa cggcgagcaa ttagccggct
     4321 tgcgcgacct gtttgccgac catgacatgg agttgtgggc gatcatcgcc gacgacggcg
     4381 agacgcgaaa tctcgcccgc gagatggggc tggcgacgcg cctttccggc agcgcgacgg
     4441 atctggacgg caacgcgttg agccatgccg tcgccgaaca tgtgcccaac ggagacggcg
     4501 ctaaagcagc tccgtcgtcg cctgtggcgg gcaacgccct gttactgcgc gagacggtgc
     4561 gatcgggccg gttggtgtgg catgaggggc atgtggttgt cctgggcgat gtgaaccccg
     4621 gtgcgcagat cgtggctacg ggcaatgtca tcgtctgggg gcggttgcgc ggactggttc
     4681 atgccggagc gagcggcgac gcgacggcga tcatctgcgc cctggatttg agtccgaccc
     4741 agctgcgcat tgccgaccat atcgccgtgg cgcccaacga cagccacgcg gcttccatcc
     4801 cggagcaggc gaccatccgc gaagggcaga ttgtggcgga accctggcgg cattgagtgg
     4861 gacagtaggt cagtaggaca gtggatcagt aaatcagtgg gtcagtggga tagttggtca
     4921 gtggatcagt actgaatact gaatactgat ctactgaata ctgatctact gaaatcgtag
     4981 cggaagtacg aaaggattga acctatgagt gcacaagtcc tgacagtaac ctccggcaag
     5041 ggaggcgtgg gaaagacgac agtgacggcc aatctggcct cggcgctggc catgctgggc
     5101 aagcgtgtga cagccatcga cgccgatatc ggtctgcgca atctcgacgt ggtcatgggt
     5161 ctggaaaatc gcatcgtcta tgatctggtc gatgtggtcg aggggcgctg tcgcctgcgc
     5221 caggcgatga tcaaggacaa gcgtcaaccc gaactgtatc tcatcccggc ggcgcaaacg
     5281 cgcgacaaga acgccgtcag cccgtcggac atggtgctga tttgcgatca gttgcgcgag
     5341 gagatggact atattgtcat cgattcgccg gcgggcatcg agcgcggctt ccgcaacgcc
     5401 gtggccccgg ctgacaaggt gctgattgtc acaaaccccg aagtatcggc cgtgcgcgac
     5461 gccgaccgta tcatcggcct gatcgaggcc gaggagaagg gaccgggcca actgatcctc
     5521 aaccgcgtca agcccgccat ggtcagccgg ggcgatatgc tctcggtcga tgatgtgctg
     5581 gatatcctgg ccgtgaacct gctgggcgtc atccccgaag acgagcgcat cctcgtggcc
     5641 agcaaccagg gtatgccact ggtgatgcac ggggtgaacg gcgccagcgc cggcgaggca
     5701 ttccgcaatg tggcccgtcg cctggatggg gaagacgtac cgtttttgga cctgacgcca
     5761 aagcagacgc tgatgggccg gctaagagcg ctcatgggca gcaattccaa ttaacgacga
     5821 acgacgacga ctgctgttct tcattcgtca ctcgtcattc aggaagaaga gggacatgcc
     5881 atgaattggc tcgatcgatt gctagggaaa aatgaaacca gcggccaggt ggccaagcag
     5941 cgcctgcaga tggtgctcat ccacgatcgc gccgacttgc cccccggcat gctggagctg
     6001 atcaaggacg acatcatcca ggtcatcgcc aggcatatgg ccatcgacac cgacaaggtc
     6061 gtcgtccatc tggagcagga cacgcaggaa aaccgcctcg tggccgaaat ccccctcctc
     6121 gccggccgaa cccgccgcaa tggcgcaagc agccgataac cggcgtagct ttccctcaat
     6181 ttccgattct caacctgtgt aatctgtgaa atctgtagtt cctcccctag gggacgagct
     6241 acaatccaca gatatgcttt caaggacatc agtctggcga tttttcgatg tgtggctgtt
     6301 ggcggccgtg ttgctgctga cgggctacgg catcctgatg atccgcagcg ccatcaccgg
     6361 cgcgcccgcc ttcgccggct atccacaaca gcaactcatc tgggccatcc tggggctggt
     6421 cattctcctg gtcacggcgg ccatcgacta tcgcgtgctc acctcggccc actggtggat
     6481 ctacgccggg ctggtcggcc tgctggtgct ggtgatcgtc accgggcagg tgaacaacga
     6541 cgctcgccgc tggattcaga tcactccctc gttccagata cagccgtcgg agttcggccg
     6601 cattttcatc tccatcacct tcgcccaatt cctggccaac cggcggcagc tcatcgcccg
     6661 cttcgacaat accatcgtca cgctggccta cttcgccatt cctgtcggcc taatcttcat
     6721 tcagcccaac ctgggcatga ccattctgtt catgttcctg tggttcatca tgatctgggt
     6781 ggccgggctg ccgctgcgcc acttcctcat gctcggcatg attgggctgg cggtgggggc
     6841 ggccgtcttc cctttcctgg ccgattacca gaagggacgc atcaccacct tcctgaaccc
     6901 cgaagaagcc agcgccgacg acgtgttcaa catcgaccag gcggagatca gcatcggctc
     6961 cggcgggctg ctgggcaagg gttatctgga gggcacacag agccagctcg gcttcctgcg
     7021 cgcccagcat actgacttca tcttctccgt cgtcaccgag gagatggggc tgctgttcgg
     7081 ctcgctggtg gtgctggggc tgatggggtt catcttgctg cgcatcctgc gcgcggcgtc
     7141 gctcagcccc gacccagccg gaaaactcat ctgcgtcggc atcgccgcca tcctcttctt
     7201 ccagacggcc gtcaacgttg gcatgaacgt gcgcgtgctg cccgtcaccg gcctgaccct
     7261 gccgttcgtc agctacggcg gcag
//
LOCUS       DAOMQG010000149         7272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4392_length_7272_cov_10.698074, whole genome shotgun sequence.
ACCESSION   DAOMQG010000149 DAOMQG010000000
VERSION     DAOMQG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4392_length_7272_cov_10.698074"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLC06_09350"
     CDS             complement(<1..670)
                     /locus_tag="PLC06_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRO90690.1"
                     /translation="MNPKIEQLRQLKQQAYQGGGADKMAARKEKGKLSARERIEVLLD
                     KGSFREIDSLVVHRETNFGMDKKKIPGDSVVTGWGTIDGRLVFVFSQDFTVFGGSLSG
                     VHAEKICKVMDMAMRNGAPMIGINDSGGARIQEGVVSLAGYADVFLRNTLASGVIPQI
                     SVIMGPCAGGAVYSPALTDFIFMVKDSSHMFITGPDVVKAVTGEDVTFEELGGAMTHN
                     KISGV"
     gene            922..1932
                     /locus_tag="PLC06_09355"
     CDS             922..1932
                     /locus_tag="PLC06_09355"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRO90691.1"
                     /translation="MALAACREKRYLSGMMNDTKPDWDQAWQGMEPEQPRPRRAIYVM
                     ALAAAIFLLLGACTIGYFILQQRTTSEPGLILPGEETATPTGMETTTAATDISSGNPS
                     IAPTVTLPAELATPPQTSAGLDAVALRATTPIIIDGSAAEWVGLPVFSSPHIVFTGDS
                     WDGSDDLAASWQMAWDDTALYLMVNVADDIHAQNQLGNQTFRGDSIELQIDADRAGDF
                     GPSLSPDDFQIALSPGDFNNVPPSAFRFQGTSGGSMLDAPGPHSIMVAATPSETGYLL
                     EAAIPWRDLNISPSAGLVIGLAVNVNDNDRPGTAVQELMKSSAPGRRFGDPTSWGTLT
                     LE"
     gene            2041..2445
                     /gene="msrB"
                     /locus_tag="PLC06_09360"
     CDS             2041..2445
                     /gene="msrB"
                     /locus_tag="PLC06_09360"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRO90692.1"
                     /translation="MSYKVQKSDAEWRAQLTPEQYRVTREKGTERPFTGALYRNEETG
                     VYTCVGCGAELFSSEQKYDAGCGWPSFWAPLVAENIDEETDRSFFMVRTEVHCANCGA
                     HLGHVFDDGPQPTGIRYCINSASMGFVKADED"
     gene            complement(2482..3426)
                     /locus_tag="PLC06_09365"
     CDS             complement(2482..3426)
                     /locus_tag="PLC06_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HRO90693.1"
                     /translation="MEYRRLGNAGMKVSAIALGGWINYGEGKVAADTAREVVLAAYNQ
                     GINFYDLADVYGNGGAEEQMGAILGQFPRHALVISTKVFYPMSEDVNDRGLSRKHIME
                     SIDRSLRRLGVEYIDIYFCHRPDPETPILETARAMDDLIRRGKVLYWGTSEWSGEQIR
                     EACDLCERYGLYPPQVEQPKYSMLRRERVEKEILPVTEPRGMGLTVFSPLGMGMLTGK
                     YDDGIPEATRFSREEWAAKQYVTDENVARVRRLKSVADSLGISRAQLALAWTLRQPGV
                     SSAIIGATKPSQILDNAAAGDITLSPDVIETIEGILVS"
     gene            complement(3470..3958)
                     /locus_tag="PLC06_09370"
     CDS             complement(3470..3958)
                     /locus_tag="PLC06_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015197993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO90694.1"
                     /translation="MSEQDNELTKDSVISLREVTKDNLNDVLRLKVKPGQEKFVADNA
                     VSIAQAHFEEKAWFRAIYADETPVGFLMLYDDPETSDYFLWRFMIGARYQGLGYGHTA
                     LAMLIEYVRALPGATELLLSYVPEAGSPEPFYRSLGFKDTGEIHDDEHVMRYEFSPET
                     SV"
     gene            4096..5400
                     /locus_tag="PLC06_09375"
     CDS             4096..5400
                     /locus_tag="PLC06_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90695.1"
                     /translation="MSIAVFCGLFLMAAGFTHRAEAQGNPTNDILQLVNQVRAEYGLP
                     AVTYNPALAAAAQNHANFIAQNAIYSHIGINGSTWQDRAQAAGYTGYAGENLVGGTKL
                     TPQQGVTWWRNSAIHFSNMLNPRWTEAGVGFAVGSGQNFYVMVFGTPHDAPPPRAEQQ
                     VIDVPFIVAPIELAQPNEDGSIIHTVLEGHTFWAIAARYEVPLADLYLFNGMNEDSVL
                     NPGDKLTIRLADGQLPPPTPTPPANHIVREGESLWSIAATYNTNLDTLLWLNQMPPDA
                     VVHPGNEVKVRLLPGEAPPPTPTPQLTHIVKSGDTALGIALLYGLSLDELAAYNNMDP
                     NAILQIGQELMIRPPTPTPAPTTTPTPTVTPEIVILETATPARSPTPKATAVAMVATR
                     TTETEKAGVNWGNVLGIGAMVAGLGLTILAAVAIMYLWRNDK"
     gene            5480..5896
                     /locus_tag="PLC06_09380"
     CDS             5480..5896
                     /locus_tag="PLC06_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRO90696.1"
                     /translation="MELPATVDYGMRIRLALPYEDAIEKVTAALKTEGFGVLTQIDVK
                     ATLKQKLDVDFRRYIILGACNPSLAHRAFSTELEIGLLLPCNVVVYEGESGAGSIVSI
                     IDPVMMLGIGINPGLSAVGEEASARLRRVLNVLESL"
     gene            6216..>7272
                     /locus_tag="PLC06_09385"
     CDS             6216..>7272
                     /locus_tag="PLC06_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRO90697.1"
                     /translation="MGQSLRILFLTAEAAPFAKVGGLGDVGGSLPKALHAMGHDVRVV
                     MPAYHNIEAGYPGVESMPLQLNVPTGSGMIQAGAFEGKLPGSEVPVYFIAKADLFNRD
                     TIYGYRDDPYRFAFFSRAAFSLTESIGWQPDVLHAHDWHSAPAIVWLATAGQANPRYR
                     DVGTVFTIHNLAHQGLTSWNIFDYLQLYTHSLTEESYGEVNFMARGIYHANLINTVSP
                     SYAREIMTPDGGARLDGLLRSRQRDLHGILNGIDYDTWNPSTDKRLPSPFDKDSLDLR
                     EENRRTLQATVGLPQIPNIPILAIISRLDWQKGLEITGHPIHLLLNGYSGPAQFIVLG
                     TGSPEYENMFSRLASYHR"
BASE COUNT         1523 a         2329 c         1953 g         1467 t
ORIGIN      
        1 ccacgccgct gatcttgttg tgggtcatcg ccccgccaag ctcctcaaac gtgacatcct
       61 caccggtcac cgccttaacc acatcggggc cggtgataaa catatggctg ctgtccttca
      121 ccatgaagat gaaatcggtc aacgccggtg aatagaccgc cccgcccgcg cacggcccca
      181 tgatgacgct gatctggggg ataacgcccg aagcaagagt attgcgcaga aacacgtcgg
      241 catacccggc caggctgacc acgccttcct ggatgcgcgc cccgcccgaa tcgttgatgc
      301 caatcatcgg tgcgccattg cgcatggcca tatccatgac cttgcagatc ttctcggcat
      361 ggacgccgct gagactgccc ccgaacaccg tgaaatcctg gctgaacacg aaaacaagtc
      421 gcccgtcgat tgttccccag ccggtgacga cgctatcgcc ggggatcttt ttcttgtcca
      481 tgccgaagtt ggtctcgcgg tggacgacca gcgagtcgat ctcacggaat gaacctttat
      541 ccagcaagac ctcgattcgc tcgcgcgccg agagcttgcc tttctccttg cgcgccgcca
      601 tcttgtccgc gccgccaccc tgataggctt gctgttttag ctgtcgaagt tgttcaattt
      661 tggggttcat agggtctatc tcaacgatct cctcttttgc cgagagccgg aagggtcatc
      721 attatgcagt tgcgggccag gatgtcattg acgccgccct tgcgagacaa tatggtcggc
      781 gaaggggtgc cgggtaatgg cggcgaactc attgtgaatc attatatgca aaccgatagg
      841 gcactccaaa ttgatgcatc tccgacaatt tacagaaccc cattgcccat agcccattgc
      901 cggccactcc gggaaaccac gatggcgctt gccgcctgcc gtgaaaaacg ctacctttcc
      961 ggcatgatga acgatacgaa gccggattgg gatcaagcat ggcagggcat ggagcctgaa
     1021 caaccacggc cgcgacgggc aatctatgtg atggcgctgg ccgcggcgat ctttcttttg
     1081 cttggcgcgt gcacgatcgg ctatttcatc ctgcaacaac gcaccacgtc ggagccaggt
     1141 ctaattctgc cgggcgaaga gacagccacg ccaaccggta tggaaacgac cacagccgca
     1201 actgatatct cttcgggaaa cccatccatt gcccccacgg tgacgttacc ggcggagctt
     1261 gcgaccccac cgcagacgtc ggccgggctg gatgccgtcg cgctgcgagc gaccacgcct
     1321 atcatcatcg acggcagcgc ggccgaatgg gttggtctgc cggtcttttc ctcgccccac
     1381 attgttttca ccggcgattc ctgggacggc agcgacgatc tggccgcctc atggcaaatg
     1441 gcctgggatg acaccgccct ctacctgatg gtcaacgtcg ccgacgacat ccacgcccag
     1501 aaccagctcg gcaatcagac ctttcgcggc gacagcatcg agctacagat tgacgccgac
     1561 cgtgccggcg actttggccc gtcgctcagc cccgatgatt tccagatcgc gctctcgccg
     1621 ggcgatttca acaacgtccc gccttcggct tttcgcttcc aggggacatc aggcggctcg
     1681 atgctcgacg cgcccggccc ccatagcatt atggtagccg ccacgccgag cgaaacaggc
     1741 tatctgctgg aagcggctat cccctggcgc gacctcaaca tctcaccctc tgccgggctg
     1801 gtcattggcc ttgccgtcaa cgtgaatgac aacgaccgcc cgggcacggc cgtccaggag
     1861 ttgatgaagt ccagcgctcc tggccggcgt ttcggcgacc ccacaagctg gggcacgttg
     1921 acgctggaat gacccagggg gcaccatgaa ttatgacaga gagattacag ccgtgtccga
     1981 tctaatgatt ctccaacgtg aacttcatat gatcagggga gttcaattca ggagcgagat
     2041 atgagttaca aggtacaaaa gtcggacgcc gaatggcggg cacaactgac gccggagcag
     2101 tatcgcgtca cgcgtgagaa aggaacggag cggccgttca ccggcgcgct ctaccgcaat
     2161 gaagaaaccg gcgtttatac ctgcgtcggt tgtggcgctg agttgtttag ttcggagcaa
     2221 aagtacgacg ccgggtgcgg ctggcccagc ttctgggcgc cgttggtggc cgagaacatc
     2281 gacgaggaaa ccgatcgcag cttcttcatg gtgcgcacgg aggtccattg cgccaactgc
     2341 ggcgcgcatc ttggccacgt gttcgacgat ggcccgcaac ccacaggcat tcgttattgc
     2401 attaattcgg cgtcgatggg tttcgtcaag gcagacgaag actagaagtc gcagaggcta
     2461 cagatcaaac tgtagcctct tttagctcac aagaatcccc tctattgttt cgataacatc
     2521 cggcgaaagg gttatatcgc ccgcggccgc gttatccagt atctgtgatg gtttggtagc
     2581 gccgatgatg gcgctgctga cgccaggctg gcgaagcgtc caggccaggg ccagttgcgc
     2641 acgactaatg cccaatgaat cggcaaccga cttgagccgt cggacgcgag ccacattctc
     2701 gtcagtcacg tactgtttcg ccgcccactc ctcccgacta aagcgagtcg cttcgggtat
     2761 cccgtcatcg tatttgccgg tcagcatgcc catgcccaac ggggaaaaca cggtcaatcc
     2821 catgccgcga ggctcggtga cgggcagaat ctccttctcc actctttcgc gccgtaacat
     2881 cgaatatttc ggctgctcca cctggggcgg atagaggccg taacgctcgc ataggtcaca
     2941 ggcttcccgg atttgctccc ccgaccattc cgacgttccc caatacagca ccttgccccg
     3001 acgaatgaga tcgtccatgg cgcgggccgt ctccaggatc ggcgtctccg ggtccggccg
     3061 gtgacaaaag tagatgtcga tatactccac gccaagccgt cgcaaactgc ggtcgatgct
     3121 ctccatgata tgctttcgag acaggccgcg atcgttcaca tcctcgctca tcggatagaa
     3181 gaccttggtg gagatgacga gcgcgtggcg cgggaactgc cccaggatcg cccccatttg
     3241 ctcctctgcc ccgccattgc cgtatacatc ggccagatca tagaagttga tcccctgatt
     3301 gtaggcggcc agcacgacct cgcgcgccgt atcggccgcc accttcccct cgccataatt
     3361 gatccagccg cccagggcga tggcgctgac cttcataccc gcgtttccta accgtctata
     3421 ttccatactt gttatcgctt tccgtatgag ctgtgtggcc aacagcagtt cataccgacg
     3481 tctcagggct aaattcatac ctcatgacat gttcatcgtc atgaatttcg cccgtatcct
     3541 tgaagcccag gctacgatag aacggttccg ggctgcccgc ctcagggacg tagctgagca
     3601 aaagttccgt ggcgcccggc agcgcccgaa cgtactcgat gagcatggcc agtgccgtat
     3661 ggccatagcc caacccctga tagcgcgccc cgatcataaa ccgccacagg aaataatcgc
     3721 tcgtctccgg atcatcatag agcatcagaa aacccaccgg ggtctcatcg gcatagatcg
     3781 ctcgaaacca ggccttctcc tcaaagtgcg cctgggctat cgacaccgcg ttatcggcga
     3841 cgaacttctc ctgccccggc ttaaccttga gccggagaac gtcattcagg ttatctttcg
     3901 tgacctcgcg caaactaatc acagaatctt tcgttagttc attgtcctgt tcactcataa
     3961 tccatgacaa tatcgcaatc caccggctat gcaagcccgc gccggaagtt gcgcctcctc
     4021 tgggataacg ccggataata gcctcttgtc actgataaga ttcaagctat attccaggat
     4081 cgtatggtga aacgattgag tatagccgtt ttttgcggcc tgtttctgat ggctgccggc
     4141 ttcacccata gagccgaagc ccaggggaac ccgaccaacg atattctgca actggtgaat
     4201 caggtgcggg ctgagtatgg cctgcccgcc gtgacgtata acccggcact ggcggccgct
     4261 gcccaaaatc acgcgaactt catcgcccag aacgcgattt acagccacat cggcatcaac
     4321 ggcagcacgt ggcaggaccg cgcccaggcg gcaggctata ccggctatgc cggcgagaat
     4381 ctggtcggcg gtacgaagct gacccctcag caaggcgtga cctggtggcg caacagcgcc
     4441 atccacttca gcaacatgct caacccgcgc tggaccgaag ccggggttgg gttcgccgtc
     4501 ggcagcgggc agaacttcta tgtcatggtc ttcggcaccc cccacgacgc gccgccgccg
     4561 cgagcggagc aacaggtgat cgatgtgccg ttcattgtcg cgccgatcga gctggctcag
     4621 cccaatgaag atggctccat cattcacacc gtcctggagg gccacacgtt ttgggccatc
     4681 gccgcgcgct atgaggtgcc gctggccgac ctgtacctgt tcaacggcat gaatgaggac
     4741 agcgtcctga atcccggcga caagctcacc attcgcctgg ccgacgggca actgccacca
     4801 ccaacgccca caccgcccgc caaccacatc gtgcgcgaag gtgaatcctt gtggtccatc
     4861 gccgccactt ataacaccaa tcttgatacg ctgctctggc tgaaccaaat gccgccggat
     4921 gctgttgtgc atcccggcaa tgaggtaaaa gtgcggctat tgcccggcga agcgccgccg
     4981 ccgacaccga cccctcaact gacccatatc gtgaaaagcg gcgatacggc tctgggcatc
     5041 gcgctcctct atggcctgtc gctcgacgaa ctggcggcct acaacaatat ggatcccaac
     5101 gccatattgc agatcggcca ggagctgatg atccgcccac cgacgcccac ccccgcgccg
     5161 acaacgaccc ccacaccaac cgtcacgccc gaaatcgtga tcctggagac cgcaacaccc
     5221 gcgcgttccc ccacgcctaa ggcgacggcc gtcgcgatgg tagccacccg gaccacggaa
     5281 acggagaagg caggcgtcaa ctggggtaat gttctcggga tcggcgcaat ggtcgccggg
     5341 cttggcctga ctatcctggc ggctgtagcc atcatgtacc tgtggcgcaa cgataagtaa
     5401 gacatccctt gctcgcctcg tctaccggtt aacgcatggc cggattggcc gttcgtaatt
     5461 tcctggagga gctaaacaaa tggagctacc tgcaaccgta gactatggca tgcgcattcg
     5521 ccttgccttg ccgtatgaag atgctatcga gaaagtgacg gccgccctga aaaccgaagg
     5581 gttcggcgta ctcacccaaa tcgatgtgaa agccacattg aagcaaaagc tggacgtcga
     5641 ttttcgtcgc tatatcatcc tcggcgcgtg caacccatcc ctcgcccatc gcgccttttc
     5701 caccgaactg gagattggcc tcctgttgcc gtgcaatgtc gtcgtctatg agggcgaaag
     5761 cggcgcagga tcgatcgtct cgatcatcga tcccgtcatg atgttgggca tcgggatcaa
     5821 ccccggcctg tcggccgtcg gcgaggaagc gagcgccagg ctgcgccgcg tgctaaatgt
     5881 gctggaatcg ttgtaaaaga caccctctat tcaatccgac gtcatccgcg ccggcccatg
     5941 cacaaccccc atatggagat tgttgctcaa cagggccggc gcggtttgtt ttccaggcgg
     6001 gccaacctgt catcacctgt atttcatctc tgtcctgtca ggagatatag gcggatcgag
     6061 tatacgctct ctaatttgta aaactcggca cactcaggga caatatactc agtccgttgg
     6121 cgaaattgac gccattagct gtaatcaaac ccaatcacag ataccctcga ttcgggcagg
     6181 cgtccgcatc ggggcatgag acgagagcta tcaaaatggg ccaatctcta cgcatcctgt
     6241 tcctgacggc cgaagccgcc cccttcgcca aagtcggcgg attgggcgat gtcggtggct
     6301 cgctgcccaa agcgctgcac gcgatgggcc acgatgtccg ggtggtcatg cctgcttacc
     6361 acaacatcga ggccggttac cccggtgtgg agagcatgcc cctgcagctg aacgtgccca
     6421 ccggttcggg gatgatccag gccggcgcgt tcgagggcaa gcttcccggc agcgaagtgc
     6481 ccgtctactt catcgcaaaa gccgacttgt ttaatcgtga tacgatctat gggtataggg
     6541 atgatcccta ccgcttcgcc tttttcagcc gggcggcctt cagtctcacc gaatcaatcg
     6601 gctggcaacc cgacgtactc catgcccatg attggcactc ggccccggct atcgtatggt
     6661 tggccaccgc cggccaggcc aacccgcgct accgcgatgt cggcactgtc ttcaccatcc
     6721 acaatctagc ccatcagggt ctaaccagct ggaacatttt cgattatctt cagctctaca
     6781 cccattcatt gaccgaggaa tcttacggcg aggtcaactt catggctcgc ggcatctacc
     6841 acgccaacct cattaacacg gtcagccctt cctacgcccg cgagatcatg acgcccgatg
     6901 gcggcgccag actggacggc ttgctccgct cccggcaacg tgacctgcac gggattctca
     6961 acggcatcga ctacgatacc tggaacccct ccaccgacaa gcgcctgccc agcccattcg
     7021 acaaggactc cctcgatctg cgtgaggaga accggcgcac actacaggcg accgttggcc
     7081 tgccccaaat acctaacatc cccattctgg cgatcatctc ccgcctcgac tggcaaaagg
     7141 ggctggaaat cacggggcat cccatccatc tgctcctgaa tggttactcc ggtccggccc
     7201 agttcatcgt cctcggcacc ggcagtcccg aatacgagaa tatgttctcg cggctggcca
     7261 gctatcatcg gg
//
LOCUS       DAOMQG010000150         7265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4396_length_7265_cov_8.615534, whole genome shotgun sequence.
ACCESSION   DAOMQG010000150 DAOMQG010000000
VERSION     DAOMQG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_7265_cov_8.615534"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLC06_09390"
     CDS             <1..722
                     /locus_tag="PLC06_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90698.1"
                     /translation="RHDRVTGITRMIDLLPNGENPWEGGSFSAISADGNRIVYRSLDG
                     GLVTGDTNQTFDIFVYDVAAGTTRRVSVDSNGMQGSKMANRPTISADGRYAAFDTTSS
                     IVTGDSNSRSDIFVHDLVTGATTRVSVKTGNLQANNHSTQASLSGDGQFVAFTSPATN
                     LIASDTNGQSDVFLHDRVTGITRRVSLTADGGQATGGASTAPRLSADGHSVVFVSTAR
                     NLIPGLKPGSKGQIYLHMLGE"
     gene            complement(832..1770)
                     /locus_tag="PLC06_09395"
     CDS             complement(832..1770)
                     /locus_tag="PLC06_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRO90699.1"
                     /translation="MSNTSSKSHRNTILWALLVTLLWSSSWVLIKVGLDGSLPPLTFA
                     GLRYSLAFLMLLPLVLFNAKQRQSLATLSRATWWRLTVLGLLLYAITQGAQFVGLSLL
                     PAATLTLLLTLTPIMVALLSILHGDEAATAGQWAGTLLSMVGAGIYFFPMVLPAGQML
                     GLAVGALSLVTNSVAALMGREINRDGRLSPLLITTVSMGIGGLLLLVVGVATQGLGQI
                     DARQWAIIIWLAVANTAFAFTVWNRTMQTLTAVESSIIANLMLPQIAIMAWVFLGEAL
                     TARQIVGLVLVAIGTLVVQLRRARPRIPIAAVIEAE"
     gene            complement(1874..4273)
                     /locus_tag="PLC06_09400"
     CDS             complement(1874..4273)
                     /locus_tag="PLC06_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO90700.1"
                     /translation="MHPTTILPDNFGSALRFLRKRARLTQEELGRAVGYSREQIARLE
                     NGSRLPDLAVIAALFVPALLLERERALVEQFLALAGQTRHGAQVTITRRKETRIQVVQ
                     ETEADTVVQPQYRPPAPLLPLLGRQAEVEELRDRIRTARLITIIGAPGIGKTRLALEV
                     ANVVRSTFADGAAFISLAEAQTAADIPYAALRHLSITPAGGQSPASAVMADLASRDLL
                     LVVDNCEHVLDGTPLFAEWLAGAPRLKLLCTSRVPLDLYGEYEWPLAPLPTPDLAESP
                     DLARWGQTAAMRLLLDRAQAVDPDFELTEDNLLPLASLCVALDGLPLALELAAVRLRD
                     LDPAQLIRQLLTLRGHAQLSSTWLQQTRRNVAERHRTLLAAIDWSEQLLPPDTRVAFH
                     HLGVFVGGCDEAAAREIAGADRATLDQLARANLIRLENGRVSLLETLRAHAHEQLAAE
                     GGLAAAQLSHAGFYTRFAQEVFTGLVGDEQAAWMARARADHDNCLAALRRAIAESDGA
                     TAIAIAGSLWWFWVRRGLFAMARELLTAALQLPATNLADRARALNGLAAFCLADEDYE
                     ANLVCHAEGLALRRQLNDADGIATVLHNMGLTAIITGDYERAMAWLDESVVVCPDCDP
                     TSAWAHMGLIAQETQDLPAARRWLEMAYARTTTTPAGWMQAFVMNYLADVLREMGEYD
                     EATRLAEESLRYFTELEDSYYLPDAQLTLAQIALDRGDYDAAAGLAALAGEQYEVRDD
                     AAFMAGVLLIQAELARIDGRPDAATLLLERSRTLRRTVTRAVSPREQAQYDRMARMLE
                     A"
     gene            4437..5099
                     /locus_tag="PLC06_09405"
     CDS             4437..5099
                     /locus_tag="PLC06_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90701.1"
                     /translation="MKHRIVLFLSLWVVLLTVACGGSEAATTPAEPPAGDTALPNPTE
                     PNGEEVAADNVALEPDAQTAATDTGCEEYFRFCTTGTLSGTIEATAAGGVGANIETCA
                     AWAAPGEARFLNLPMMLAAGEAQVTVALTAISAYTGPGTYELVAVTTEGMPDAFPAID
                     VAGRTFNNGEPSTAVVTVNADGSGTLEAHDLVEIASVQKSNPDPDARVDFTMQWTCQD
                     IN"
     gene            5197..7080
                     /locus_tag="PLC06_09410"
     CDS             5197..7080
                     /locus_tag="PLC06_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO90702.1"
                     /translation="MKRIIRILVVGLLLSGLTGTKARAATTDAVVGTGTPASCDETAF
                     DAALATVQAGTRGTITFDCGPASHTIEIYNSATISKEVTIDGGGLIRLFAQGSSPMFV
                     KPRFFEVSAGGWLTLNDMVLEGGRGPAGDGWGSQGGSIVVWGAGQLDMQGTTILNSAS
                     TAWGGAIANEGGVVRVQDSIISGSSAKWGGAYNGANGYDIFINATVVGSISAEGGGGV
                     RLWNTIDSSITDSTISGHTTAGSGGGIENIGGQVTIRGSYIEGNAATLWGGGVKNSNN
                     GDRTGWILIENSRIADNVSGINGGGIESQGSLTLRTTRVTDNTAVAGGGILSWDGQLT
                     IDGTSVVENSADKGGGIYAHGGGVVIDNSQVADNVATEGGGLYLTEIAGAGANNWANI
                     TASDITGNRANSGSGILAAHAYVTVSQSEIFGNGALAVYLWQTVSGGSYMVVTQSSIH
                     DNTGSGFYNGENSTLTIGNSTISQNGEWGVWAGQNSIQTLLGFSTVRDNATGQIRRTG
                     GQLVLEASAIDRGTTATPNCVTDAGLPAPQGGGNMANDNSCGAPVTPNSDLDLGPLEL
                     NGRATPGHWPQPGSALIDAAACGIYKTDQHGMACPQGTACDVGAVEVGPDGGRIFVPA
                     IMR"
BASE COUNT         1538 a         2297 c         2202 g         1228 t
ORIGIN      
        1 ttcgtcatga ccgcgttacc ggcatcaccc gcatgatcga tctcctgccc aatggggaga
       61 acccatggga aggcggttcg ttttccgcta tctcggccga cggcaatcga atcgtctacc
      121 gctcccttga tggcggatta gtgaccgggg atactaacca gaccttcgac atcttcgtct
      181 atgacgttgc tgccggcacc acacgccggg tttcggtcga ttccaatggc atgcaaggca
      241 gcaagatggc aaatcgcccg acaatttcag ccgacggcag gtatgcggca ttcgacacca
      301 cttcctcaat tgtgaccggc gacagcaact cccgcagtga tattttcgtt cacgatctgg
      361 tcacgggcgc tacgacacgg gtctcggtca agaccggcaa tttacaggcc aacaatcatt
      421 cgactcaggc ctcgttgtcg ggcgacggcc aattcgttgc gttcacctcg ccggccacga
      481 acctgatcgc cagtgacacc aacggtcaga gcgatgtctt tcttcatgat cgcgtgaccg
      541 gcatcacccg tcgtgtttcg ctgacggccg atggaggaca agctaccggt ggcgcatcga
      601 ccgcgccgcg tctatcggcc gatggtcatt ctgttgtttt cgtgtcgacg gcccgtaacc
      661 tcatccctgg tcttaaaccc ggttccaaag gccagattta tttgcatatg ctaggcgaat
      721 gaagggacta gagctgcgac tcagccctcc atgcgatgga tatgttcgtc gggcggaaag
      781 tcggccgact tgacgggcac gggcacaaaa tcgacttcct gatcgtattc gctactcagc
      841 ctcaatcact gcggcgatcg ggatcctggg ccgggcgcgc cgcaactgca ccaccagcgt
      901 gccgatggcg accagcacca gcccaacgat ttgccgcgcc gtcaatgcct cgcccagaaa
      961 cacccaggcc atgatggcga tctgcggcag catcaaattg gcgatgatgc tcgactctac
     1021 ggccgtcagc gtctgcatcg tgcggttcca gaccgtgaag gcgaaggcag tattagccac
     1081 cgccagccaa ataatgatcg cccactgccg ggcgtcgatc tggcccaacc cctgagtcgc
     1141 cacgccgacg acgagcagca gtaacccgcc tatgcccatg ctgaccgtcg tgatcagcag
     1201 cggcgacagg cggccgtcgc ggttgatctc tcgccccatc agcgccgcca ccgagttcgt
     1261 caccagactc agcgccccca cagccagccc cagcatctgc ccggccggca gaaccatcgg
     1321 gaagaaataa atgcccgcgc ccaccatcga caacagcgtg ccggcccatt gcccggccgt
     1381 cgccgcctcg tcgccatgaa gaatgctcag cagagccacc ataatgggcg tcagggtcaa
     1441 cagcagcgtc agcgtggccg caggcagcag cgaaaggccc acgaactgcg ccccctgcgt
     1501 gatcgcatag agcagcaggc ccagcaccgt caaccgccac caggtagcgc gcgataacgt
     1561 ggccagcgat tgccgctgct tcgcgttgaa caataccagc ggcaaaagca tcagaaacgc
     1621 caggctatag cgcaacccgg cgaacgtgag cggcggcagg ctgccgtcca gcccgacctt
     1681 gatgagaacc cacgacgacg accacaacag cgtgaccagc agcgcccaga ggatcgtatt
     1741 acgatggctt ttggaagatg tgtttgacaa gtagtgtgtt cctcacattc ctgaataatg
     1801 gcaggccccg caagccggca aacctgtcag cattctttcg ccgatgcgac aggaattggc
     1861 agagttgacc agatcaggcc tccagcattc gcgccatccg atcatattgc gcttgctctc
     1921 gaggcgagac ggcgcgggtg accgtccggc gcagcgtgcg cgagcgctcc agcaacagcg
     1981 tcgctgcatc aggtcgcccg tcaatgcgag ccagttccgc ctgaatgagc agaacccccg
     2041 ccataaaagc cgcatcgtcc ctgacctcgt actgctcgcc ggccagcgcc gccaacccgg
     2101 cggccgcgtc atagtccccc cggtccagcg caatttgcgc cagcgttagc tgggcatcag
     2161 gcaaatagta gctatcctcc agttccgtga aatagcgcag gctctcttcg gccaggcgag
     2221 ttgcctcgtc atactcgccc atctcgcgca gcacgtcggc caggtagttc atgacgaacg
     2281 cctgcatcca tcccgccggc gtcgttgtcg ttcgcgcata ggccatctcc agccaacgcc
     2341 gcgccgcggg caagtcctgc gtttcctggg cgataaggcc catatgggcc caggccgatg
     2401 tcggatcgca atcggggcaa acgaccacgc tctcgtccag ccaggccatt gcccgctcgt
     2461 aatcaccggt aataatagct gttaatccca tattatgaag aacagtggcg atgccatcgg
     2521 cgtcattaag ttgccggcgc agggccagcc cctccgcgtg gcagaccaga ttggcttcat
     2581 aatcctcgtc ggccagacag aaggccgcca gaccgttgag agcacgagca cgatcggcca
     2641 aattggtcgc gggcagttgc agcgccgccg tcagcaattc gcgcgccatc gcgaacaaac
     2701 cgcgccgaac ccagaaccac cacaagctcc cggcaatagc gatagccgtc gccccgtcgc
     2761 tctcggctat ggcccggcgc agcgccgcca ggcagttatc gtgatcggct cgcgctcgcg
     2821 ccatccatgc cgcttgctcg tcgccgacca acccggtaaa cacctcttgg gcaaagcgcg
     2881 tatagaagcc ggcatgcgac agttgggcag cagccaaccc gccttcggcg gccagttgct
     2941 catgggcatg ggcgcgaagc gtctccagca gggagacgcg gccgttttcc aggcgaatga
     3001 gattggcgcg ggccagttga tccagcgtcg cccggtcggc cccggcgatc tcccgtgctg
     3061 ccgcctcatc acaaccgccg acaaacaccc ccagatgatg aaacgccacc cttgtgtcag
     3121 gcggcaggag ttgctcgctc caatcgatag ccgccagcaa tgtgcggtgg cgctcggcca
     3181 cgttgcggcg cgtctgttgt aaccaggtgg acgaaagctg ggcgtggccg cgcaaggtca
     3241 acagttgccg gatcagttgc gccggatcga gatcgcgcaa cctcaccgca gccagttcca
     3301 acgccagcgg cagaccgtcg agagccacgc acagcgaggc caacggcagc agattatctt
     3361 cggtcaactc gaaatcgggg tcgaccgctt gcgcccgatc caacagcagg cgcatggccg
     3421 ctgtctgccc ccagcgcgcc aaatcgggcg attcggcgag atcgggcgtg ggcagcgggg
     3481 ccagcggcca ctcgtactca ccgtacaagt cgaggggaac gcggctggtg cacagcagct
     3541 tcaggcgcgg cgctccggcc aaccattcgg cgaagagagg cgtcccatcc agaacgtgct
     3601 cgcagttatc cacgaccagc aacaagtcgc gcgaggccag atcggccatc acagccgacg
     3661 cgggcgattg accgccggcg ggtgtgatgg acagatggcg caaggcagca taaggaatgt
     3721 cggccgcggt ttgcgcctcg gccagagaga tgaaagccgc gccatcggca aaagtcgatc
     3781 ggacaacatt ggccacttcc agagcaaggc gtgtcttgcc gatgcccggc gcgccgatga
     3841 tggtgatcag ccgtgcggtt cggatgcgat cccgtagttc ttccacttcc gcctgccgac
     3901 ccagtaacgg cagcaagggc gcgggcgggc ggtattgagg ttgcactacc gtatcggctt
     3961 cggtttcctg gaccacctga atgcgcgtct ctttgcggcg cgtgatggtg acctgggcac
     4021 cgtggcgtgt ctgcccggcc agcgcgagga attgttcaac cagtgctcgc tctcgttcca
     4081 acaacaacgc ggggacaaac agggcggcga tgacggccag atcgggcaat cgtgagccgt
     4141 tctccagccg ggcgatttgc tcgcggctat agccaaccgc ccggcccaac tcttcctggg
     4201 tcaggcgggc gcgtttgcgc agaaagcgca gggcagagcc aaagttatcg ggcaatatcg
     4261 tcgtcggatg catagccaac cgggaattct actgcttccg cgagttgtca caaaatgtca
     4321 ccaatagcca cggcaaagcc gatattgttc ggcaatgaaa agcagacagg tcattgtgac
     4381 gttggagcag atcgagacga tcacaatcac catcatcaca caaggagaaa tgaagcatga
     4441 aacatcgaat cgtcttgttt ttatcgctgt gggtcgtttt gctcaccgtg gcgtgtggcg
     4501 gcagcgaagc agccacgaca ccggccgaac caccggcggg ggacaccgcc ctgccgaacc
     4561 cgactgaacc caacggggaa gaggttgcgg ccgacaacgt cgcgcttgag ccggatgcgc
     4621 agacagccgc gaccgacacc ggttgcgaag aatacttccg tttctgcacc accggtacgc
     4681 tcagcggaac catcgaggcg accgctgcgg gcggcgtggg cgccaacatt gaaacttgcg
     4741 cggcatgggc cgcgccgggc gaagcgcgct tcctgaatct gccgatgatg ttggctgccg
     4801 gcgaggcgca agtcacggtc gctctcacag ctattagcgc ctatacggga ccgggtacct
     4861 atgaactggt tgccgtcacc accgaaggga tgccggacgc cttcccggcg atcgacgttg
     4921 cagggcgaac gtttaacaac ggcgaaccct cgacggccgt cgtgactgtc aacgccgacg
     4981 gctccggcac gttggaagcg cacgatttgg tggagatagc ctcggtgcaa aagtcaaatc
     5041 ccgatccgga cgcgcgggtg gactttacca tgcaatggac gtgtcaggat atcaactagc
     5101 gcacatcacc ggtaaacagc cgcacttttg gcaagtcatt tctgaatgcg accacgctga
     5161 gatcgacata caaaccagta cacggaacaa acgagaatga agcgaataat ccggatttta
     5221 gtcgttggac tactgctatc ggggttgacc ggcaccaagg cgcgggccgc aacgactgat
     5281 gcagtcgtcg gcacgggcac accggccagt tgcgatgaga cggccttcga cgcggcactt
     5341 gccacggtgc aagccggcac gagaggcaca atcaccttcg attgtggccc cgccagccat
     5401 accatcgaga tctacaactc ggcgaccatc agcaaggaag taaccattga cggcggtggg
     5461 ctgatccgcc tgttcgcgca gggcagttcg ccgatgttcg tcaaaccgcg cttcttcgaa
     5521 gtgagcgccg gcggttggtt aacgttgaat gatatggttc tcgaaggcgg gcgaggcccc
     5581 gcgggcgacg gctgggggtc gcagggtggg tcaatcgtcg tctggggtgc gggtcagctg
     5641 gacatgcaag gaacaacgat cctcaacagc gccagcaccg cctggggcgg ggccatcgcc
     5701 aatgagggcg gggttgttcg cgtccaggat tcgatcatca gcggcagcag cgccaaatgg
     5761 ggcggcgcct ataatggcgc gaacgggtat gacatcttca ttaacgccac cgtggtcggc
     5821 tcgatctcgg ccgagggagg aggtggcgtg cgcttatgga acaccatcga cagcagcatt
     5881 accgacagca ccatcagcgg ccataccacc gccggctcgg gtggcggcat tgaaaatatc
     5941 ggcggccagg tgaccattcg cggcagctat atcgagggca atgcggcgac cctatggggt
     6001 ggtggcgtca agaacagcaa caacggcgac aggacaggct ggatactgat tgagaatagc
     6061 cgtattgccg acaatgtcag cggcataaat ggcggcggca ttgagagtca aggatctcta
     6121 acgctgcgca ccacacgggt gaccgacaac acggccgtgg cgggcggcgg aattctcagc
     6181 tgggacgggc aattgacaat agacggcaca tcggtggtgg aaaactcggc cgacaagggc
     6241 ggcggcatct atgcccacgg cggtggggtt gtcatcgaca actcgcaagt cgccgataat
     6301 gtcgcaacgg aaggcggcgg gctatacctg acggagatcg ccggagcggg ggccaacaat
     6361 tgggccaaca tcaccgccag cgacatcacc ggcaaccggg cgaattcggg gagcggtatt
     6421 ctggccgccc acgcttacgt gaccgtgagc caatccgaga tattcggtaa tggggccttg
     6481 gccgtctact tgtggcagac cgtatcgggc ggcagctaca tggtcgtcac ccagagcagt
     6541 attcacgaca ataccggcag cggcttctac aacggcgaga acagcaccct gaccatcggc
     6601 aacagcacca tcagccaaaa cggcgagtgg ggtgtgtggg cagggcagaa cagcatccaa
     6661 accttgctgg gcttcagcac tgtgcgcgac aatgcgacgg gtcagattcg gcgcacggga
     6721 ggacaactcg tactggaggc ttcggccatc gaccgcggca cgacggcaac gcccaactgt
     6781 gttactgacg ccgggttgcc cgcgccacag ggcggcggca atatggcaaa cgacaactca
     6841 tgtggcgcgc cggtcacgcc caacagcgat ctcgacctgg ggccgctgga attgaacggc
     6901 cgcgcgacgc cgggtcattg gccgcagccg ggcagcgccc tgattgacgc ggccgcgtgc
     6961 ggcatataca aaaccgacca gcacggcatg gcatgcccac aaggaacggc ctgcgacgtg
     7021 ggggcggtgg aggtcggccc ggacggcgga cgaatctttg tgccggcgat catgcgttaa
     7081 tcccgatgaa cagtcgcggg gtggctcagc taccccgcga cctcgacggc gacgccacga
     7141 cattaaccca tcgcatatct attcaatctt cgccgccgga taagacccca gcaccttcag
     7201 gaacgaggca tgccgcagga tgcccagcag cgcctcgcgc accggctcgt catcttcatg
     7261 cccct
//
LOCUS       DAOMQG010000151         7260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4401_length_7260_cov_6.685913, whole genome shotgun sequence.
ACCESSION   DAOMQG010000151 DAOMQG010000000
VERSION     DAOMQG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4401_length_7260_cov_6.685913"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLC06_09415"
     CDS             complement(<1..947)
                     /locus_tag="PLC06_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO90703.1"
                     /translation="MLYTDLEDILGKVRASLEMDDLAGAVATIEALRAPDQADLFYEL
                     DDEDQTALLPHLDPEDSADILEELEDEDAARIVASLTREDAVRIIDEMEPDEAADLLV
                     ELEPEAQEYVLSRLEDGAELRPLLLHPEDTAGGLMTSEFLALGRRMTVGEALAALRTW
                     RPDAEDTYDVYLIDRYGRLAGEVTMRRLLRADHNTLLMDIVEEESVSALVGTDQEEVA
                     RIMSRYDLVSLPVVDAHDVLVGVITIDDVVDVLEEETTEDFQRFGGTEPLEQPYLDSG
                     VLTVTRKRIGWLLLLFITETLTGSVLRHFEEEMAAVVSLA"
     gene            1746..2378
                     /locus_tag="PLC06_09420"
     CDS             1746..2378
                     /locus_tag="PLC06_09420"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HRO90704.1"
                     /translation="MNIRGAFASLPPAAALLGVNTIPALGWLRETWSAETTMILYYLE
                     TAAMVVLVGLMVRLAVPAVDERGERITVRRNRLARDYWLVMGGFALAIGVFMAVILLL
                     FLDTPVPWRAVGAVMGIIIALQLFAYGWEAWRMWPLNIADAESLVNRDMGRIAILHLG
                     VLFGIFLAMVRSDWFVWPFFILKTISDMGRLLAPALGLPVTGFATKEPGD"
     gene            complement(2375..2662)
                     /locus_tag="PLC06_09425"
     CDS             complement(2375..2662)
                     /locus_tag="PLC06_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRO90705.1"
                     /translation="MNATPLPLTVFGGIDCDDTERVVALLDALGFPYRLVIIEEDPAA
                     ERFVIFINNGYRSTPTLVFGGGNYKIVLTEPTDEELMSLLRQARPAAPDAI"
     gene            complement(2855..5131)
                     /locus_tag="PLC06_09430"
     CDS             complement(2855..5131)
                     /locus_tag="PLC06_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HRO90706.1"
                     /translation="MHRKLIAVFLLTIILILSVSSVALGQDDGLLEADRLTALGGNGD
                     NLQHPLGKQQWGLRQIALQQRLQGVEADNGIMRLGVGRYVELEREDEDSIWTVLGEFG
                     DQVHPSYGGDPGPLHNTIAQPDRNFDNTTIWEPDFSQAYYLDLLFNDAPGAITMRNWY
                     IEQSSGRYAVNGDVTDWVKVPYNTARYGNNACGSSVCATVWYFVQDSVNAWYQEMLDN
                     GWTEADIADYLSRFDVWDRYDFNGNGNFDEPDGYIDHFQSVHAGEGNEAGGGAFGDDT
                     IWSHRWYAFYNLIGSAGPAFNMMGGIQIGNSDYWIGDYTIEPENGGVGVFAHEFAHDL
                     GIPDLYDTGGGENSTGFWTVMSQGSWGSGNTTDIGSKPNHFGAWEKFQLGWLDYDVAF
                     AGERSTHILGPAATTTQRAQGLFILLPDKEVSADIGTPYAGSYFYYSGSGNDLDNFMV
                     REFDLPAGASLSAMANFQIEQDWDYAYVVASGDGGATWDMLPTNLSTDDDPNAQNQGN
                     GITGSTGGAWVELTADLSAYSGPTLVGFRYWTDVAAAEPGFSVDEIAITGSPVDGAES
                     DAGWTFDPADGGFRVTSGTESAYYFNAYVLEYRRYWGFDDVLRTGPYNFGFLDNPNLQ
                     NWVEHFSYQDGLLISYWDDSYSDNNTSQHPGGGLILPIDAHPQTMYRADGVPWRPRVQ
                     AFDSTFGVWRTDAITLNRNSMPSFHPGRRAVRIFNDRLQYWNPETPAAGVMNPHTGTR
                     VRITGLSQSGNFMHLDIR"
     gene            5765..6955
                     /locus_tag="PLC06_09435"
     CDS             5765..6955
                     /locus_tag="PLC06_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO90707.1"
                     /translation="MTLTIDSRRTAHFHPSDLHALARVITDATTGIVDLVEDIHMRVA
                     PRRLLGPAGKIAPFTYQTIRDATRLIGGGATLTLAQIGELLERRENSPQRRAVLSILN
                     GLWGDYLHETNSPLALPMSFAYGEGAPKSKIVVLVHGLCMNSDGWRRGGEAGDPDGPV
                     DYGAALARDLGYSPVYLNYNSGLHISSNGRALAIMLEALVDVWPVPVEELVIIGHSMG
                     GLVTRSACYYGETAGHAWLDPLKATIFLGTPHHGVPLEKQGNLLNMLIDSMPFVAPFG
                     RLGRRRSAGITDLRYSNLVDEDWAGRDRFAPGPDRRCHVPPPAGARACAVAAVLGSRA
                     DSAAARLLGDGLVPLDSALGMHPAADRCLGFDDSNSWVGYGMNHVDLLGRTEVYMRMA
                     DWLA"
     gene            complement(6925..>7260)
                     /locus_tag="PLC06_09440"
     CDS             complement(6925..>7260)
                     /locus_tag="PLC06_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90708.1"
                     /translation="GATGPGEQDEQQKRQAAEPPADHDKACNPFRLEDRYPRHDKTPI
                     IVWNPVQPGKPRFLTDDSLAQLGKIRYSRGRAIRGTRMKNIGMRTTPRGRMAAEAQTD
                     QASQSAIRM"
BASE COUNT         1260 a         2177 c         2357 g         1466 t
ORIGIN      
        1 gccagcgaca ccacggcggc catctcttcc tcgaaatggc gcagcacact gccggtcagc
       61 gtctcggtga tgaacaataa tagcagccag ccgatgcgct tgcgggtgac cgtcagcacg
      121 cccgaatcca gatagggctg ctccagcggc tcggtgccgc cgaagcgctg gaagtcctcg
      181 gtcgtctcct cttccagcac gtcgaccacg tcgtcgatgg tgatgacgcc gacgaggaca
      241 tcgtgggcgt ccacgacggg cagcgacacc aggtcatagc gcgacatgat gcgggcgacc
      301 tcttcctggt cggtgcccac cagcgccgag acggactcct cctcgacgat atccatcagc
      361 agcgtgttgt ggtcggcgcg cagcaggcgg cgcatggtga cctcgcccgc cagccggccg
      421 tagcggtcga tcagatagac gtcgtaggtg tcctcggcgt cgggccgcca ggtgcgcagc
      481 gccgccagcg cctcgccgac ggtcatgcgc cgccccaggg ccaggaattc gctggtcatc
      541 aggccgccgg cggtgtcctc cgggtgcagc agcagcggcc gcagctcggc cccatcctcc
      601 agacgggaga ggacgtactc ctgcgcttcc ggctccagct cgaccagcaa gtcggccgcc
      661 tcgtccggct ccatctcgtc gatgatgcgc acggcatctt cccgcgtcag gctggcgacg
      721 atgcgggccg cgtcttcgtc ttccagctct tccaggatgt cggccgagtc ctccgggtca
      781 agatgcggca ggagcgcggt ctggtcttcg tcgtccagtt cgtagaacag gtcggcctgg
      841 tcgggggcgc gcagggcttc aatggtggcc accgcgccgg cgagatcatc catctcgagc
      901 gaggcgcgaa ccttgcccag gatatcttcc aggtctgtgt acagcatggg acacctccag
      961 ggtactaagc cacccggagg cggaacgagg gtctgccaac cagggggtca gttattcggt
     1021 tgacaggggc ggggtctgtc tatgacaccc gccaggttca gatttttcgt tcacttcttt
     1081 ctccgtcttt tggccggtgg gtcggtatga aagtaaggcc ccactgtcgc cagacgcaag
     1141 gggaaattat agtttattgc ggggggttgt caatgatgat cgcggggtcg atcagggcaa
     1201 tcgcatgaaa agatgaagga gcgaacagaa cgcggacgac atggatttcc ctgatttaaa
     1261 tggacagaaa taacccttaa gatccgacaa aacaggctga tgtcgggaat cctgcctcgc
     1321 agccccccgc catatccgtg ttcattcacg ttaattcacg ttaaatcacg ttaattcacg
     1381 ttaaatcata aatcgcgtta attcacgtta aatcacgtta attcacgtta aatcgcgttc
     1441 attcacgtta aaccgcgttc attcacgtta aatcgcgttc attcacgtta aaccgcgttc
     1501 attcacgtta aatcgcgttc attcacgtta aaccgcgttc attcatgtta aatcgcgttc
     1561 attcgcgttc attcgcgttc attcgcggtc caatattctt catgcgtctg ccctgaagat
     1621 ggcgtccgat ggcgtctttg cgccccgatt ccctgttcga taatatagcg aatacgcttg
     1681 gggaggagat gcacagggcc gcccgcgggc ggaataaagg ctcgacggat gggcgaaagg
     1741 ttattatgaa tatcaggggc gcgtttgcat cattgccgcc ggctgcggcg ctgctggggg
     1801 tgaataccat tccggctctc ggctggctgc gcgaaacgtg gtcggccgaa acgaccatga
     1861 tcctctacta tctggagacg gccgccatgg tggtgctggt cggcctgatg gtgcggctgg
     1921 ccgtgcccgc cgtggacgag cgcggcgagc gtatcaccgt caggcggaac cggctggcgc
     1981 gggattactg gctggtcatg ggtggttttg cgctggccat cggcgtgttt atggcggtca
     2041 tattgctgtt gttcctggac acacccgtcc cgtggcgggc ggtgggcgcg gtgatgggga
     2101 ttatcatcgc gctgcagctg tttgcctatg gctgggaggc gtggcggatg tggccgctga
     2161 acattgccga cgccgaaagc ctggtgaacc gcgacatggg ccgcattgcc atcctgcatc
     2221 tgggggtgct gttcggcatt tttctggcca tggtgcgctc ggactggttc gtctggccgt
     2281 ttttcatcct gaagacgata tcggatatgg gccgcttgtt ggcgccggcg cttggccttc
     2341 cggtaaccgg ttttgccaca aaggagccgg gggactagat cgcgtcgggc gccgccgggc
     2401 gcgcctgccg cagcagactc atcaactcct cgtctgtcgg ctcggtcagg acgatcttgt
     2461 aattgccgcc accgaacacc agcgtggggg tgctacgata gccgttgttg atgaagatga
     2521 caaagcgttc ggccgcgggg tcttcttcga tgatgaccag ccgatagggg aagcccagcg
     2581 catcgagcag ggcgacgacg cgctcggtgt cgtcgcagtc gatgccgccg aaaacagtga
     2641 ggggcagggg ggttgcgttc atgcggttga tcttaccgga ttggggcgtg gttgtcgatg
     2701 aaggacgacc accgcgactc ctgtcgcgcg accggattgg gcgtggttgt cgatgaagga
     2761 cgaccaccgc gactcctgtc gcgcgactcc tgtcgcagct gtgaacactc cggcgggtca
     2821 gccttgcggc cgacccgccg gaacccacta atgcttagcg aatatccagg tgcatgaagt
     2881 tcccggattg gctcaggccc gtgatgcgga cccgcgtgcc ggtgtggggg ttcatcacgc
     2941 ccgctgccgg tgtctccggg ttccagtact ggagccggtc gttgaagatg cgcaccgccc
     3001 gccgtccggg atggaacgac ggcatgctgt tgcgattcaa cgtgatcgcg tccgtccgcc
     3061 agacgccgaa ggtggagtcg aacgcctgga cgcgcggccg ccacggcacg ccgtccgccc
     3121 ggtacatcgt ctgcggatgg gcgtcgatgg gcaggatcag gccgccgccg ggatgctggc
     3181 tggtgttgtt gtccgagtag gagtcatccc agtagctgat gagcaggccg tcctgatagg
     3241 agaagtgctc gacccagttc tgcagattgg ggttatcgag gaagccgaag ttatatggcc
     3301 cggtgcgcag cacgtcgtcg aagccccagt agcggcgata ttcgaggacg taggcgttga
     3361 agtagtacgc gctctccgtg ccgctggtga cgcggaagcc gccatcggcc gggtcgaacg
     3421 tccagccggc gtcgctctcg gcgccatcga cgggcgagcc ggtgatagcg atctcgtcga
     3481 cgctgaagcc cggctcagcc gcggccacgt cggtccagta gcggaagccg accagcgtgg
     3541 ggccgctgta ggccgagaga tcggccgtca gctccaccca ggcgccgccg gtgctgccgg
     3601 tgatgccgtt gccctggttc tgggcattgg gatcgtcgtc ggtcgagagg ttggtgggca
     3661 gcatatccca cgtcgcgccg ccgtcgccgg aggccaccac gtaggcgtag tcccaatcct
     3721 gctcgatctg gaagttggcc atggccgaaa gcgatgcgcc ggccggcagg tcaaactcgc
     3781 ggaccatgaa gttgtcgagg tcgttgccgg agccgctgta gtagaagtag cttcccgcat
     3841 agggcgtgcc gatatcggcg ctgacttcct tgtcgggcag caggatgaag aggccttgcg
     3901 cccgctgggt ggtggtagcc gccggcccaa ggatatgggt cgagcgctcg ccggcgaagg
     3961 cgacgtcgta atcgagccag cccaactgga acttctccca cgcgccgaag tggttcggct
     4021 tgctgccgat gtcggtggtg ttgccgctac cccatgaacc ctgggacatg accgtccaga
     4081 agccggtgga gttctcgccg ccgcccgtgt catacaggtc ggggatgccc aggtcgtggg
     4141 cgaactcatg ggcgaagacg cccacgccgc cgttctccgg ctcgatggtg tagtcgccga
     4201 tccagtaatc ggagttgccg atctggatgc cgcccatcat attgaaggcc ggaccggccg
     4261 agccgatcag gttatagaag gcgtaccagc gatggctcca gatggtgtcg tcgccgaacg
     4321 cgccgccgcc ggcctcattg ccttcgccgg cgtggaccga ctggaagtgg tcgatgtagc
     4381 cgtccggctc gtcgaagttg ccgttgccgt tgaagtcgta gcgatcccag acgtcgaacc
     4441 ggctcagata gtcggcgatg tcggcctcag tccagccgtt gtcgagcatc tcctgatacc
     4501 aggcgttcac cgaatcctga acgaagtacc agaccgtggc gcaaacgctg ctgccgcagg
     4561 cgttgttgcc atagcgggcg gtgttatagg gcaccttcac ccaatcggtc acatcgccgt
     4621 tcaccgcgta gcggcccgac gactgctcga tgtaccagtt gcgcatggtg atggcgccgg
     4681 gcgcgtcgtt gaacagcaag tccaggtagt aagcctggct gaagtccggt tcccagatcg
     4741 tcgtgttatc gaaattacga tcgggctggg cgatcgtgtt atgaagcggg ccggggtcgc
     4801 cgccatagct gggatgaacc tggtcgccga attcgccgag gacggtccag atgctgtctt
     4861 cgtcctcgcg ctccagctcg acatatcggc cgacgccgag gcgcatgatg ccgttgtcgg
     4921 cctcaacccc ctgcaggcgc tgctgcaggg ctatctgacg caggccccat tgctgcttgc
     4981 cgagggggtg ctgcaggttg tcgccgttgc cgccgagcgc cgtcagccgg tcggcttcca
     5041 acagcccatc atcctggccc agggccacgg acgagacgct caatatcaaa ataatggtca
     5101 gcaggaaaac cgcgatcaat ttgcggtgca tgggtctact cctgattttc ttcattctta
     5161 atgctcatac agccgattcg aatgggggct gccttccgcg tggcccccgg cgattacgag
     5221 gtggttagtg tgggttaatt ggcagtagat gcacctccgc tggagtggga taatgaggcg
     5281 ggatggagcg gcgcgaggcg cccgaagttg gccgcaggtg aacgaaacgg gcgcaattcc
     5341 ggctggagcc ggctgcgaga tcgccaccaa ataggccatc aaaaacaaga tatacggcca
     5401 tgagcttgtg ttcgcccgac aaatgacgga tgacgtgaac gcgacacccg tgctgccccc
     5461 gctacggcct gctttggggg tctattcttt tgacggactt catgtatttc gcgatccaac
     5521 cggctacaac tgacgatcgc gcaaatgtgg ttacgaaaat agtgtaatgg tgttggcggc
     5581 acgcgtcaat atttttattg ggcaaattcg gcattctgtc gggaaaaacg gcacgaaaaa
     5641 cgggctggag cgctcgccgg cacgcatctc gttcgcgagt tttcccgtcg agccgataca
     5701 acgagcgcat ctatcggcat gggtgcgggt tatagctatt cggaaacctt tatggaacag
     5761 tcctatgacc ttgacaattg attcccggcg aaccgcccat tttcatccct ctgacctcca
     5821 cgctctggcg cgggttatca ccgatgccac gacgggcatc gtggatttgg tggaagacat
     5881 tcatatgcgc gtggccccgc gccggctgct tggcccggcg ggcaagattg ccccgttcac
     5941 ctatcagacc atccgcgacg cgacgcggct catcggcggc ggcgccaccc tgactctggc
     6001 gcagatcggc gaactgctgg aacggcgcga gaactcgccc caacgccggg ccgtgctgtc
     6061 gatcctcaat ggcctgtggg gcgattatct gcacgagacg aacagcccgc tggcgctgcc
     6121 gatgtcgttt gcctatggcg agggcgcgcc aaaaagcaag atcgtcgtgc tggtccacgg
     6181 tctgtgcatg aacagcgacg gctggcggcg gggcggcgag gcgggcgacc ccgacggccc
     6241 cgtcgactat ggcgcggcgc tggcgcgcga cctgggttat tcacccgttt atctcaatta
     6301 caacagtggg ctgcacatct ccagcaacgg ccgcgccctg gcgattatgc tggaggcatt
     6361 ggtcgatgtc tggccggtgc cggtggagga gctggtcatc atcggccaca gcatgggcgg
     6421 gctggtgacg cgcagcgcgt gctactatgg cgagacggcc ggccacgcct ggctcgaccc
     6481 gctgaaggcg acgatcttcc tgggcacgcc ccaccatggc gtgccgctgg agaagcaagg
     6541 caacttgctc aatatgctga tcgattcgat gcccttcgtg gccccgttcg gccgtcttgg
     6601 gcggcggcgc agcgcgggca tcaccgacct gcgctacagc aatctggtcg acgaggactg
     6661 ggccgggcgc gatcgatttg cgccggggcc ggaccggcgc tgccacgtgc cgccgccggc
     6721 cggggcgcgc gcctgtgccg tcgccgccgt gctaggcagc cgggcggatt cggccgcggc
     6781 tcgcctgctg ggcgacggcc tcgtcccgct cgacagtgcc ctgggcatgc acccggcggc
     6841 cgaccgctgc ctcggtttcg acgactcaaa cagttgggtc ggctatggca tgaaccacgt
     6901 tgatctgttg ggtcgaacag aggtttacat gcgaatggcc gactggctgg cctgatctgt
     6961 ttgtgcttcg gcggccatcc tgcccctcgg tgtcgtccgc attccaatat tcttcatgcg
     7021 cgtgccccgg atcgcgcggc ccctgctata gcgtatcttg cccaattgcg ctaaactgtc
     7081 atctgtaagg aagcgcggct taccaggctg cacagggttc catacgatga tgggggtttt
     7141 gtcgtgccgg ggataacgat cttcaagcct gaatggattg cacgccttgt catgatccgc
     7201 cggcggttcg gccgcctggc gcttttgctg ctcgtcctgt tcgccggggc ctgtcgcccc
//
LOCUS       DAOMQG010000152         7258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4404_length_7258_cov_11.242677, whole genome shotgun sequence.
ACCESSION   DAOMQG010000152 DAOMQG010000000
VERSION     DAOMQG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4404_length_7258_cov_11.242677"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PLC06_09445"
     CDS             <1..1053
                     /locus_tag="PLC06_09445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRO90709.1"
                     /translation="MFIVDAHLDLAYNALVKGRDPRLGVAAIRAGEESPKDIATVGLP
                     EMRRAGVGLIFATLFVSPSSTPFEREPSEHTYDTPEQAHKLAMAQVDFYKRMIDEDET
                     LRLVTNLAGLDEVTKSHEAGGNPLLGLVLLMEGADCIREPKEAEFWYEQGVRLIGPAW
                     DDTRYCAGAWRDSKQGLSKDGYALLEEMAGLGMILDLTHMSEVATFQSLDAYPGTIVA
                     THANARAIVPRERQLSDRQIRLIGERDGMVGAVLANFFLKADYVRGQKESVLVAQVAA
                     HIDHVCQVIGDARHAGLGTDLDGGFGVRDIPDPMDSIADLPMIAAALREKGYADADVA
                     GIMGENWVALLRRAWE"
     gene            1126..1524
                     /locus_tag="PLC06_09450"
     CDS             1126..1524
                     /locus_tag="PLC06_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO90710.1"
                     /translation="MDIIVDASVILAVVMNEPAKPAMISFTRDAKLIAPTSIHFEIGN
                     ALSAMFRRRRVTLEQATEAVDVYQSIPIRFVNVGLINSLVIAHHLNIYAYDAYLMRCA
                     EKYRAPLLTLDTSLRRRAVEYGLQVVEVGE"
     gene            1521..1763
                     /locus_tag="PLC06_09455"
     CDS             1521..1763
                     /locus_tag="PLC06_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRO90711.1"
                     /translation="MTTIYTYSEARQNLASLLDEATEEGEVLVQRRDGRMFAIRPVAP
                     SASPLDVDGIDIDMTADEIMAFIAEGRRYGLPEGKE"
     gene            complement(1844..2269)
                     /locus_tag="PLC06_09460"
     CDS             complement(1844..2269)
                     /locus_tag="PLC06_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRO90712.1"
                     /translation="MSRKAKLARYLLDTSALLAHFRHETGWEEVQRIFEDPGAAVFLA
                     AVSLAEFARRLNDLGAPREEIDQIVSDYELLFAAIAPVDARTAKAAYEIVQSTPARLP
                     LIDALIAAVAQELDAILVHRDQHMAAIPEAVLQQQHLAG"
     gene            complement(2253..2495)
                     /locus_tag="PLC06_09465"
     CDS             complement(2253..2495)
                     /locus_tag="PLC06_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRO90713.1"
                     /translation="MITTVTRKNMITIPAEVSRIFGIKPGWKLEWQPLEGTDEILVRV
                     IPDRGELARRLAGTGKKYSPERDAVAELITERESEG"
     gene            complement(2673..6749)
                     /gene="dnaE"
                     /locus_tag="PLC06_09470"
     CDS             complement(2673..6749)
                     /gene="dnaE"
                     /locus_tag="PLC06_09470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRO90714.1"
                     /translation="MLGTATKPPVPEEHQPFVHLHVHSEYSLLDGLSRLEHLAKRAKA
                     LNQPALALTDHGVMFGVMPFYQACKNHGIKPIIGIESYVAARGMTDQDPQLDRERTHL
                     LLLAENRTGYLNLLQIASSAQLDGFYYKPRIDLDFLAAHSEGLICTTGCMAADIPRAI
                     GAGDMTKAHALMGQYIDIFGPERFFIELQEHSIPELTAINKTLMEMQPRYNLRFLATN
                     DVHYTTPEEASPHDVLLCIQTGATVNDAKRMRFSDQGYYLKSHAEMHRLFGHIPGALD
                     NSLLIAEMCNVNLDTKGYHLPIFDVPAGYDAPGYLRQLCEAGLIQKYGEERARTDEKL
                     RARLEHELRIIGGMGFDTYFLIVWDLCQYARRVDIWWNVRGSGAGSLVAYTLGITGID
                     PIANGLIFERFLNPGRVSMPDIDLDYPDDRRSEMVEYAVNKYGQDKVAQIITFGTLGA
                     RAAIRDVGRALDVPLPEVDAVARLVPAISGKPVKIDNVLDKDHEFYSADLDARVKNEP
                     RIKELIDTAREIEGVARHASSHAAGVIISDKPLVEYVPLHRPTSGEAGLGGIRSVSQW
                     PMEIIEKIGLLKVDFLGLSTLTVMRKAAQLIEQRHGVRYTMDNIPSDIGMTGPGKDSD
                     KGMEAAFDMLGRGDVLGVFQVEGSGMRKLMMEMKPRRFDHIIAAISLFRPGPMENIPE
                     YIRRMHAALNEGRDLAAYHTPELKPILQDTYGILVYQEQIIRIAAELAGYEPGEADMI
                     RKAVSKKKRDLMDEHKAQFTAGAMARGFSQEVCDAIWSDIEFFARYGFNKAHAADYAV
                     ICCQTAYLKAHYPVEYIAALLTVGRHDTENVAKYITDARQLGITVAPPGINRSMLDFT
                     IEDVVEEKGRKGERGITPSPLHPLSPAQKATIRYGLAAIKNAGEGAVQLVIDEREANG
                     PFRDPADLADRVDLRRVGKRALESMARVGVFDEWGSRARILDALDRMVGHSGSTHAAA
                     EAGQMTLFGGTGGGKIDVALLRPEKEIKPVDRREMLDWEKELIGVYLTEHPLEHKLAE
                     LKSIVTARTGELDTNWNGKPITLAGMVAGLRTLNTKKGQPMAFVTLEDLDGKIDLVMF
                     PKVWAAHREMVQVNQIIVVRGTVQAEGESISIIANSVQTRLTVAKDAAAARQPAETGF
                     DEQEFVPKNPVSSDIANDYDFWPPDDDWNTAPATPTISHPVAEPAPAYTATGNGFVAA
                     ESVPPPPDEIDEEEAMTCETSEADDEPTRPGGITNYDSRRSTNLRITNDGSNDSLHMG
                     KSLKVNDSSSPYEDEPPPANGSYKMLVVEIRASGNWKEACRQSFRLAERYEGNATLRL
                     QLAGQDLVMDFPNNRVGCEAELIEKLERVPGVGRVYER"
     gene            complement(6904..>7258)
                     /locus_tag="PLC06_09475"
     CDS             complement(6904..>7258)
                     /locus_tag="PLC06_09475"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRO90715.1"
                     /translation="IPFVPHNYYCTYFVSIHQLFRRNGCRECNMTTSQNDVAELVTAK
                     DVEFEGNMLHVSLSDGRPISLPLDTISWLGWLANATPEQRANWSIEPGGYGVYWEELD
                     DGFEVMHLLNVQPLT"
BASE COUNT         1301 a         2051 c         2344 g         1562 t
ORIGIN      
        1 atgtttatcg tcgatgccca cctcgatttg gcctataacg cactcgtcaa gggacgcgac
       61 ccacggctgg gcgtggccgc aattcgcgcg ggcgaagaat cacccaagga tatcgccacc
      121 gtcggcttgc cggagatgcg gcgtgccggg gtcggcctta tcttcgctac cctgtttgta
      181 tcgccatcat ccacgccatt cgagcgcgaa ccgagcgaac acacctacga cacgccggag
      241 caggcccaca agctggccat ggctcaggtc gatttctaca agcgaatgat cgatgaggat
      301 gagacattgc ggctggtgac gaacttggcc gggctggacg aggtgacgaa aagccacgag
      361 gccggcggga acccgctgct gggtctcgtc ttgctgatgg aaggggccga ttgcatccgc
      421 gagccgaaag aggccgagtt ctggtatgaa cagggcgtgc ggctcatcgg cccggcatgg
      481 gacgacacgc gctattgcgc cggggcctgg cgcgacagca agcaagggct gtcgaaagac
      541 ggctacgcgc tgctggaaga gatggccggg ctggggatga tcctcgacct gacccacatg
      601 agcgaagtcg ccaccttcca gtcgctcgac gcctatccgg gcacgatcgt ggctacccac
      661 gccaacgcgc gggccattgt tccccgcgag cggcaattga gcgaccggca gatccgcctt
      721 atcggcgagc gcgacgggat ggtcggggcg gtgctggcca actttttcct gaaggcggat
      781 tatgtacgcg gccaaaagga gagcgtgctg gtggcgcagg tggcggccca tatcgatcat
      841 gtctgtcagg tcatcggcga cgcacgccat gccggtctgg gcaccgacct tgacggcggc
      901 ttcggcgtgc gcgacatccc cgacccgatg gacagcatcg ccgacctgcc gatgatcgcc
      961 gccgcgctgc gcgagaaagg ttacgcggat gcggatgtgg ccgggattat gggggagaat
     1021 tgggtggcgt tgttgcggag ggcgtgggaa tagaagtaga ttgcaattac tcctgcgatc
     1081 tgttatattg gttgtacaat tgtacaatca atattgattt ctattatgga cataatcgta
     1141 gatgcttcag ttatcctggc tgttgttatg aatgagccag cgaaaccggc aatgatttcc
     1201 ttcaccagag atgcgaagtt gatcgcgccc acctcaatcc atttcgagat cggtaacgcc
     1261 ttgtcggcga tgtttcgtcg ccgacgggtg acacttgaac aagcgactga ggctgtggat
     1321 gtctaccagt ctattccaat tcgttttgta aacgttggac tcatcaactc actggtaatt
     1381 gcacatcatt tgaatatcta tgcctatgat gcctatttaa tgcgatgtgc ggagaaatat
     1441 cgtgctcctc tactcacgct ggacacatca ctgcgtcgtc gggctgtcga atatgggcta
     1501 caagtcgtgg aggtgggaga atgacgacca tctataccta ttcggaggca cggcaaaacc
     1561 tggcctctct tttggatgag gcgactgagg aaggtgaagt gctggtgcaa cgtcgcgacg
     1621 gacgcatgtt cgcgatccga ccggtcgctc cctcggcgtc gccattggat gttgatggaa
     1681 tcgacattga tatgaccgcg gacgaaataa tggcgttcat tgctgagggc cggcggtatg
     1741 ggttgcctga ggggaaggag taactgcccg tcatccgccg gattgcgaaa ggatcaggca
     1801 gctgtgctgc gcggtgaggg gaatcggctt ctcaacgaca ggattaaccg gcaaggtgtt
     1861 gctgctgaag gacggcttcg gggattgcgg ccatgtgctg gtcgcgatgg accaaaatcg
     1921 catcgagttc ctgcgccaca gcggcgatga gggcatcgat gagcggcagc cgcgccggcg
     1981 ttgattggac gatttcatag gcggccttcg cagtccgggc atccaccgga gcgatggccg
     2041 cgaaaagcag ctcataatcg ctcacgattt ggtctatctc ttcgcggggc gcgcccagat
     2101 cgttcaatcg ccgggcgaac tcggccaggc tgacggcggc cagaaaaacg gccgctcccg
     2161 ggtcttcaaa aatgcgctgc acttcttccc aacccgtctc atggcgaaaa tgggccagaa
     2221 gggcggatgt atcaagcagg tagcgcgcca atttagcctt ccgactcacg ctctgtgatc
     2281 aattcggcca ccgcgtcccg ttcggggctg tattttttgc ccgtcccggc cagccgccgc
     2341 gccaattcgc cgcggtcggg aatgacgcga accaggattt catccgtccc ttcgaggggt
     2401 tgccattcca gcttccagcc cggcttgatg ccgaagatgc ggctgacctc cgccggtatg
     2461 gtgatcatgt ttttccgggt gacggttgtt atcatggggc ttatcctatt ccatgattaa
     2521 tgattttgca aggagattgt ggatagcctg ggttgcaggg caacccgtgt ccgcaatcgt
     2581 tgtcgttgtc gctgtcgctg ccgttgccgt aatcgaaaat cgatagcgat agcgattacg
     2641 atagcgattg cgattgcgat ttagacgcta catcaccgct cgtacacccg ccccacaccc
     2701 ggcacccgct ccagcttctc gatcagctcc gcctcgcagc cgacgcggtt gttggggaag
     2761 tccatcacca ggtcttgccc ggctagctgc agccgcagcg tcgcgttgcc ctcatagcgt
     2821 tcggccagcc ggaacgattg gcgacaggct tccttccagt tgcctgaggc gcgaatctcc
     2881 acgacgagca ttttgtatga gccgttggcc ggcggcggtt cgtcttcata gggcgacgag
     2941 gaatcattga ccttcaggga tttacccatg tgcaatgaat cattgctccc atcattcgta
     3001 attcgtaagt tcgtagaacg acgggagtcg taattcgtaa ttccccccgg ccgggtcggt
     3061 tcatcatccg cttcggatgt ctcacaggtc atggcttcct cttcatcgat ctcgtcgggc
     3121 ggcgggggga cgctctcggc cgcgacgaat ccgttgccgg tggcggtgta ggccggggcg
     3181 ggttcggcca ctgggtgcga gatagtcggt gtcgcgggcg cggtgttcca gtcgtcatcg
     3241 ggcggccaaa agtcgtagtc gttggcaata tcgctagaaa ccgggttttt gggaacgaac
     3301 tcctgttcgt caaacccggt ttcggccggt tggcgggcgg cggcggcatc cttggccacg
     3361 gtgagtctgg tttgcacgct gttggcgatg atggagatcg attcgccttc ggcctgcacc
     3421 gtgccgcgga cgacgatgat ctgattgacc tgtaccatct cgcggtgggc ggcccacacc
     3481 ttggggaaca tgaccagatc gatcttgccg tcgaggtctt ccagggtgac gaaagccatc
     3541 ggctggccct tcttcgtgtt cagcgtgcgc agccccgcga ccatgcccgc cagcgtgatg
     3601 ggcttgccgt tccagttcgt atccagctcg ccggtgcggg cggtgacgat gctcttcaac
     3661 tcggccagct tgtgttccag cggatgctcg gtcagataga cgccgatcaa ctccttctcc
     3721 caatcgagca tctcgcggcg gtcaaccggc ttgatctctt tctccggccg cagcagggcg
     3781 acgtcgattt tgccgccgcc ggtgccgcca aaaagggtca tctgcccggc ttcggccgcg
     3841 gcgtgggtgc tgccgctgtg gccgaccatc cgatccagcg cgtcgaggat gcgcgcccgt
     3901 gagccccact cgtcgaacac gccgacgcgg gccattgact ccagcgcgcg cttgcccacg
     3961 cgacgcagat cgacgcggtc ggccaggtcg gcgggatccc tgaacggccc gttggcctcg
     4021 cgctcgtcga tgaccagctg caccgcgccc tcgccggcgt ttttgattgc ggccaatcca
     4081 tagcggatgg tggctttttg agcaggggag agggggtgaa ggggagaggg ggtgatcccc
     4141 ctttctccct ttctcccttt ctcctccacg acgtcctcaa tcgtgaagtc cagcatcgac
     4201 cggttgatcc ccggcggggc gacggtgatg cccaactgcc gggcgtcggt gatgtacttg
     4261 gcgacgttct ccgtgtcgtg gcggccgacg gtgagcaggg cggcgatgta ctctaccggg
     4321 taatgggcct tcaggtaggc ggtctggcag cagatgacgg catagtcggc ggcgtgggcc
     4381 ttgttgaagc catagcgggc gaagaactcg atgtcgctcc agatggcgtc gcacacctct
     4441 tgcgagaagc cgcgggccat ggctccggcg gtgaactggg ccttgtgttc gtccatcagg
     4501 tcgcgcttct tcttggacac ggccttgcgg atcatgtcgg cctcgcccgg ctcatagccg
     4561 gccagttcgg cggcgatgcg gatgatctgc tcctgataga cgagaatgcc gtaggtgtcc
     4621 tgcaggatcg gcttcagttc gggggtgtgg taggcggcca ggtcgcgccc ctcgttgagc
     4681 gcggcgtgca tgcggcggat gtattcgggg atgttttcca tcggcccggg gcggaagagg
     4741 gagatggcgg cgatgatgtg gtcgaaacga cgcggtttca tctccatcat cagcttgcgc
     4801 atgccggagc cttccacctg gaacacgccc agcacgtcgc cgcggccgag catgtcgaag
     4861 gcggcttcca tgcccttgtc gctgtccttg ccggggccgg tcatgccgat gtcggacggg
     4921 atgttgtcca tcgtatagcg cacgccgtgg cgctgctcga tgagctgcgc ggccttgcgc
     4981 atgaccgtca gggtgctgag gccgaggaag tcgaccttca gcaggccgat cttctcgatg
     5041 atctccatcg gccactggct gacggagcgg atgccgccca gcccggcctc gccgctggtc
     5101 ggccgatgga gcggcacgta ttccaccagc ggtttgtcgg agatgatgac gcctgcggcg
     5161 tggctgctgg cgtggcgggc cacgccttcg atctcccgcg ccgtgtcgat caactccttg
     5221 atgcgcggct cgtttttgac tcgtgcgtcc agatcggccg aatagaattc gtgatccttg
     5281 tcgagcacgt tgtcgatctt gaccggcttg ccggagatgg ccgggacgag gcgggccacg
     5341 gcgtcgactt cgggcagggg cacatcgagc gcgcggccga cgtcgcggat ggccgcgcgc
     5401 gcgcccagcg tcccgaaggt gatgatctgg gccaccttgt cctggccata cttgttcacg
     5461 gcgtattcga ccatctccga gcggcggtcg tcggggtagt cgaggtcgat gtcgggcatg
     5521 gagacgcggc cggggttcag gaagcgttcg aagatgaggc cgttggcgat ggggtctatg
     5581 ccggtgatgc ccagcgtgta ggctaccagc gaccccgcgc cgctgccgcg cacgttccac
     5641 cagatatcga cacgtcgggc atattggcat aaatcccaga caatgaggaa ataggtgtcg
     5701 aagcccatgc cgccgatgat gcgcagctcg tgttccagcc gggcgcgcaa cttttcgtcg
     5761 gttcgcgccc gctcctcgcc gtacttttgg atcagccctg cctcgcacag ttggcgcaga
     5821 tagccggggg cgtcgtagcc ggcgggcacg tcgaagatgg gcaggtgata gcctttcgtg
     5881 tccagattga cattgcacat ctcggcgatg agcaggctgt tgtcgagcgc gccggggatg
     5941 tggccgaaca ggcggtgcat ctcggcatgg gatttgaggt agtagccctg gtcgctgaag
     6001 cgcatgcgtt tggcgtcgtt gacggtggcg ccggtctgga tgcacaacag cacgtcgtgg
     6061 ggcgaggcct cttcgggcgt ggtgtaatgg acgtcgttgg tggccaggaa gcgcaggtta
     6121 tagcgcggct gcatctccat gagcgttttg ttgatggccg tcagttcggg gatgctgtgc
     6181 tcctgcaact caatgaagaa gcgttcgggg ccaaagatgt cgatgtattg gcccatgagg
     6241 gcgtgggctt tggtcatatc gcccgcgccg atggcccgcg ggatgtcggc ggccatgcag
     6301 ccggtggtgc agatgaggcc ctcgctgtgg gcggccagaa agtcgaggtc gatgcgcggc
     6361 ttgtagtaga agccgtcgag ttgggcgctg ctggcgattt gcagcaggtt cagatagccg
     6421 gtccggttct cggccagcag cagcagatgg gtgcgctcgc ggtcgagttg ggggtcctgg
     6481 tcggtcatgc cgcgcgcggc cacatagctc tcgatgccga tgatgggctt gatgccgtgg
     6541 ttcttgcagg cctggtagaa gggcatgacg ccgaacatca cgccgtggtc ggtcagggcc
     6601 agggcgggct ggttgagcgc tttcgcccgt ttggccaaat gctccagccg gctcaggccg
     6661 tcgagcagcg agtactcgct gtggacatgg agatggacga agggttggtg ttcttccggt
     6721 acgggtggtt ttgttgcagt ccccaacatg agggtgagta taacacaggt gttcggatgg
     6781 gggtctactg tgtcttaaag aattgggggt tttaaggtca acactcgtac tcgaatctta
     6841 tatgctcgtt gaccaaattt ctggcattgc aaaaacaatc cactacgaat catagacaag
     6901 cgttcaagtg agcggctgca catttaacaa atgcatcact tcaaagccgt cgtccaattc
     6961 ctcccaataa accccatacc cacccggctc aatggaccaa ttggctcgct gctccggcgt
     7021 ggcgttggcc agccagccca gccagcttat cgtatcgaga ggcaggctta tcgggcggcc
     7081 gtcgctgagc gacacatgaa gcatgttgcc ttcaaactca acatctttag ctgttaccaa
     7141 ttcagcaaca tcattttgag atgtagtcat attgcattcc cggcaaccat ttcgtctgaa
     7201 aagttgatgt attgacacaa agtatgtaca ataataatta tgcggtacga atgggatg
//
LOCUS       DAOMQG010000153         7228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4424_length_7228_cov_9.256239, whole genome shotgun sequence.
ACCESSION   DAOMQG010000153 DAOMQG010000000
VERSION     DAOMQG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4424_length_7228_cov_9.256239"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLC06_09480"
     CDS             <1..1406
                     /locus_tag="PLC06_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRO90716.1"
                     /translation="TIEATHLYDEYRLNELPRALQRNSLDYYYLSIYPSLSEMRPLAP
                     DDVPPYPATIANAYIHIPFCSGVCDFCSYYLVAVNPRRRAAIARYLEQVKAELDYHAR
                     HTTLDFSYLFFGGGTPSLIPPDTLESFLAFMAERGYLNPTAMGTFELHPEFFDDQATA
                     ERFLSILKRHGVGRVSVGYQFSDEAILRETNRRHTTGFMDRAMELLRESGFIVNIDLM
                     YGLSGQSFDGWEKTLAHTIGFAPDSISTYFLFVDRGTGLYEQVRRGQVTLPSHRHVQT
                     QHLMAQIALGAAGYYELPNDFWARDVGDPTTFRPERLPSASVTLPVGSGAYGYYNRVQ
                     MANVFDLGEYGRRMTAGLSPLWRGYPLTDSESFHRDVMFALKNDPYIDCSLFRTAYNR
                     SPLDQFEMIFEKLVEYGMVDVSGDRIYLTSKGRLCVEELAGLFRHPAIGPASDSLNGD
                     RLLAKHNFAMTYPPVEW"
     gene            1424..1837
                     /locus_tag="PLC06_09485"
     CDS             1424..1837
                     /locus_tag="PLC06_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRO90717.1"
                     /translation="MSERRPRVLIGKPGLDGHDRGAKVTARALRDAGMEVIYTGLRQT
                     PEMIVEAALQEDVDAIGLSILSGAHMPLMARVMELMTENEMADVPVFVGGIIPDEDID
                     ALREMGITAVFGPGASLDDVIASFRQAIAARHAGE"
     gene            2014..2886
                     /locus_tag="PLC06_09490"
     CDS             2014..2886
                     /locus_tag="PLC06_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HRO90718.1"
                     /translation="MERPGRNDPCYCGSGKKYKQCHMTADLAADREQRARSDAARDLR
                     LALSEFAAGERFDAEAGKAAAYYWDGLYTADTLSLMSPSEEERFLDWLIFDYTLPESG
                     DRLVEIYGREMGADISAHQRELLDQWADGLPMSGYELTGYDRQTLQLKEIFSGLDLEV
                     VEPSGHGNAPLGAIILGRPVEVQGHYEFFAMPAYIPPNEIADLHEKLAAARAADAAGS
                     DAEFMRRHNVLLIHHALEQAKAAGRPPVTRLDPRHAADGVRQRARHERVRIKAQTGVT
                     DNAPQPVQTHRKAI"
     gene            2891..>3468
                     /locus_tag="PLC06_09495"
     CDS             2891..>3468
                     /locus_tag="PLC06_09495"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRO90719.1"
                     /translation="MSEFTNVIVRGQTTPLNGSVTVPGDKSISHRAVMLAALAEGPSI
                     IRNWLPAGDTLATLDVVRALGVAIEVDKRSPHSWDLRVEGRGLNGLRKPGAPLNCRHA
                     GTAIRLLAGILAGQSFPSILDGSEQLRKRPMGRVTEPLRLMGADIKSEEGHAPLAIRP
                     ASLHGIEYTLHVASAQVKSSILLAGLYAAGTTR"
     assembly_gap    3469..3568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3569..4180
                     /gene="rsmI"
                     /locus_tag="PLC06_09500"
     CDS             <3569..4180
                     /gene="rsmI"
                     /locus_tag="PLC06_09500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRO90720.1"
                     /translation="LRAVDYVASEDTRKTGLLLKHFEIKKPQIAFHEHNEDKAGQRIT
                     ALLQQGLSVAVVSNAGTPGVSDPGYTLVRRAVELDLPVTMIPGPSALIMAVVLSGLPS
                     HAFTFRGFPPRKSGKRRSFLAVDRDSPHTLIFYESPYRLREFLIDALAVYGDRPAAVA
                     NELTKLFESVRRGTLSELIAQAEESEPRGEYVVVISGASADHP"
     gene            complement(4201..6288)
                     /gene="uvrB"
                     /locus_tag="PLC06_09505"
     CDS             complement(4201..6288)
                     /gene="uvrB"
                     /locus_tag="PLC06_09505"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRO90721.1"
                     /translation="MSEFKLVSDFAATGDQPAAIDTLVEGIYQGDKFQTLLGATGTGK
                     TFTIANIIQKTQRPTLILAHNKTLAAQLYSEFRDFFPQNAVSYFVSYYDYYQPEAYIP
                     RHDLYIEKETQINEEIDRLRLQAMTTLKSRKDVIIVASVSCIYGIGNPEAWGKVILEL
                     ERGGQYRRNTVLRHLVDIHYDRNDLDLRRGTFRVRGDTLHIYPAYAETAISVEFWGDI
                     VERITEFDPLTGEVLLEHTQIAIYPAREFITDEEKLARAINDIETELTDRVAFYKKED
                     RYLEAQRIEQRVMYDLEMLREVGFTPGIENYSRHLDQRPAGSRPWTLLDYFPADYLMV
                     IDESHMTIPQVRGMWGGDQSRKSILVDYGFRLPSAMDNRPLNFGEFEGLLNQVIFTSA
                     TPGPFEMAHSEQIAHQVIRPTGIIDPEVEVRPVRGQVDDLIGEVNRRIANGQRVLVTT
                     LTKRMAEDLSDYLLEMGIKVHYLHSEVATLERTEILRDLRLGVYDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSETALTQTIGRAARHVEGKVIMYADKITDSMAAAIATTN
                     SRREIQMAHNKEHGIEPRSIVKAVHVLTDELAAQQEMARGELALAESKGGYVTAADLP
                     KIELAKIISELEKEMKQAAQQLEFERAAVLRDQVVEMRQIMVLKEAGGKSDLPEWERM
                     RKLDEAGVAYEVE"
     gene            complement(6437..7201)
                     /locus_tag="PLC06_09510"
     CDS             complement(6437..7201)
                     /locus_tag="PLC06_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HRO90722.1"
                     /translation="MVAAQGTPTPAPTPTEAPTPTPAPPTVYTYRVINTFPHDPNAFT
                     QGLDFDDGRLFEGTGRWGESTLREVDLQTGAVLRSHSLDAQYFGEGITVFNGKIYQLT
                     WQELTGFIYERDTFEPLQTFSYPHEGWGITHDGTRLIVSDGSSTIRFWDPNTLQEIGN
                     ITVRDDQGPVNRLNELEYIDGEIWANIWLTDLIARISPETGDVLSYVDLTGLLDTGTL
                     TQPADVLNGIAVDDSGRLFVTGKLWPTLFEIEVVEK"
BASE COUNT         1327 a         2080 c         2230 g         1491 t
ORIGIN      
        1 tgacgatcga ggcaactcat ctctatgatg aataccgact caacgagttg ccgcgcgcgc
       61 tacagcgcaa cagcctcgac tactactatc tgagcatcta tccctcgctg tcggagatgc
      121 ggccgctcgc gccggacgac gttccgccct atccggcgac gattgccaac gcctacatcc
      181 atatcccgtt ctgctccggt gtgtgcgatt tttgcagcta ctatctggta gccgtcaacc
      241 cccggcggcg ggcggccatt gcgcgctatc tggaacaggt gaaggccgaa ctggattacc
      301 acgcccgtca cacaacgctg gatttctcct acctcttttt tggcggcggc acgcccagcc
      361 tcatcccgcc cgatactctg gaaagcttcc tggcgttcat ggccgagaga ggctatctga
      421 acccgacggc gatggggacg tttgaactgc acccggagtt tttcgatgac caagcgaccg
      481 ccgagcgatt cctgagcatc ctcaaacggc acggggttgg gcgggtcagc gtcggctacc
      541 agttttcgga cgaagcgatc ctgcgcgaaa ccaatcgccg ccacacgacc gggttcatgg
      601 atcgggcgat ggaactgttg cgggagagcg gcttcatcgt caatatcgac ttgatgtatg
      661 gattgtcggg ccaatcattc gacggctggg agaagacgct ggcgcacacg atcggcttcg
      721 cgccggactc gatctcgacc tacttcctgt ttgttgatcg cgggacaggc ctgtatgagc
      781 aggttcggcg cggccaggta acattgccgt cccatcgcca cgtgcagacg cagcacctca
      841 tggcccagat cgcgctgggt gcagccgggt attatgaact gcccaacgac ttctgggcgc
      901 gggatgtggg cgacccgacg acttttcggc cggagcgact gccgtcggca tccgtgacct
      961 tgccggtagg gtcgggggcc tatggctatt acaatcgcgt tcaaatggcg aatgtatttg
     1021 atctgggcga gtatggccgg cgtatgacgg ccggattatc tcctttgtgg cgtggctacc
     1081 cattgaccga cagcgaatcg tttcaccgcg atgtgatgtt cgctctgaag aacgatccct
     1141 atatcgattg tagtctgttc cgaaccgctt ataatagaag cccgctggac caatttgaaa
     1201 tgatttttga aaaactggtg gaatacggga tggtggatgt gagcggtgac cggatttacc
     1261 tgacctcgaa agggcggcta tgcgtcgagg agttggccgg tctgttccgc cacccggcta
     1321 ttgggccggc gagcgatagt ctgaatggcg accgattgtt ggccaagcat aacttcgcca
     1381 tgacctatcc gccggtcgag tggtgacgag aggagaatta ttcatgagcg agcgacgacc
     1441 gcgcgtttta atcggcaagc ccggcctgga cgggcacgac cggggggcca aagtgacggc
     1501 ccgtgccctg cgggatgccg gaatggaagt gatctacacc ggcctgcggc aaacgccgga
     1561 gatgatcgtc gaggctgcat tgcaggaaga cgtggacgca atcggcctca gcatcctttc
     1621 gggggcccac atgccgctca tggcgcgtgt catggagctg atgaccgaga acgagatggc
     1681 cgacgtgccg gtgttcgtgg gcggtattat ccccgacgaa gatatcgatg ccttgcgcga
     1741 gatgggcatc acggccgtct ttggccccgg tgcgagtttg gatgacgtca tcgcctcgtt
     1801 cagacaggcg attgccgctc gtcatgccgg cgaataagcc ggtcagcctg taacgggccc
     1861 ccgtttaacg actttaattc atcatacggt gcgcggccgt tcatccgtca ttctcccatc
     1921 ggtgacattt ctgctacaat tggatgccaa ttgcaattgg catgagttgt gcccggctgg
     1981 ccggcgcgac cgtttttagt tggagaaatc agtatggaaa gaccgggccg gaatgatccc
     2041 tgctattgcg ggagcggcaa aaaatataaa caatgtcata tgacggccga cctggccgcg
     2101 gatcgcgaac agcgcgcccg gagcgacgcg gcccgtgatt tgcgcctggc gctgtctgag
     2161 tttgccgccg gagagcgctt cgacgccgag gcagggaagg cggccgccta ctactgggat
     2221 ggtctctata cggctgacac cttgtcactc atgagcccat ccgaagaaga gcgtttcctg
     2281 gactggctca ttttcgatta tacgctgccc gaatccggcg accgtctcgt tgagatatac
     2341 ggccgcgaaa tgggcgcgga tatttcggct catcagcggg aactacttga ccaatgggcc
     2401 gatggattgc ccatgagcgg ctatgaactt actggatatg atcgccagac gctccagctg
     2461 aaagagattt tttccggatt ggacctggaa gtcgtcgagc cgtccgggca tggcaacgcg
     2521 ccattaggcg cgattatcct ggggcgtccg gttgaagtgc agggacatta cgagttcttc
     2581 gccatgccgg cctacattcc gccgaacgaa attgccgatc tgcatgagaa gctggccgcc
     2641 gcgcgcgcgg ccgacgcggc cggtagcgat gccgagttca tgcgccggca caacgtgctt
     2701 ctgatccacc acgccttgga acaagccaaa gcggccggtc gccccccggt aacacgcctt
     2761 gacccgcgcc atgccgctga cggtgtccgg cagcgcgcgc gccatgagcg cgtgcgcatc
     2821 aaagcccaaa ccggcgtgac cgataacgcg ccccagccgg tgcaaacaca ccgcaaggcg
     2881 atctgagttt atgtctgaat ttacaaacgt aattgttcgc ggccaaacca cgccgcttaa
     2941 tggttcggtt accgtgcccg gcgacaagtc catcagccat cgggccgtta tgctggcggc
     3001 actggccgag gggcctagca ttatccgcaa ctggctcccg gcgggggata cgctggccac
     3061 gctcgacgtc gtgcgggcgc tgggtgtggc gattgaagtc gacaagcgca gcccccactc
     3121 ctgggatttg cgcgtggaag gccgcggact gaatggcctg cgcaagccgg gcgcgccgct
     3181 caattgtcgc cacgccggga cggctattcg tctgctggcc ggaatcctgg cggggcagtc
     3241 gttcccgtcg attctggacg gcagcgagca actgcgcaaa cggccgatgg gccgggtgac
     3301 ggaaccgctg cgccttatgg gggcggacat caagtcggaa gaaggacacg cgccgctcgc
     3361 tattcggcct gcttcgctcc acggcattga atacaccctg cacgtcgcca gcgcccaggt
     3421 gaaaagctcg atcttgctgg cggggcttta tgccgccggc acgacgcgnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gcgcgcggtc gactatgtgg ccagcgagga
     3601 cacccgcaag accgggctgc tgctcaagca ttttgagatc aagaagccgc agatcgcctt
     3661 ccacgagcat aatgaggaca aggccggcca acgcatcacg gcgttgcttc agcagggtct
     3721 gtcggtggct gtcgtctcca acgccggaac acccggcgtg tcagaccccg gctatacgct
     3781 ggttcggcga gccgttgagc ttgatctgcc cgtgacgatg atccccggcc cgtcggcgct
     3841 gatcatggcg gtcgtcctgt cggggctgcc atcccatgcc ttcacgtttc ggggcttccc
     3901 gccgcgcaaa agcggcaagc ggcggagctt cctggcagtc gatcgcgatt cgccccacac
     3961 gctgatcttc tacgaatcgc cttatcgact gcgggaattc ctgatcgatg cgctggcggt
     4021 atatggcgat cgcccggcgg ccgtagccaa tgagctgacc aagctgttcg agtctgtgcg
     4081 ccggggcact ctctccgagc tgattgctca ggccgaagag tcggagccgc gcggggaata
     4141 tgtggtggtg atcagcggag cctcggccga tcacccataa cttacgaaag gccccaggcg
     4201 ttattccacc tcataggcca cgccggcttc gtccagtttt cgcattcgtt cccactccgg
     4261 cagatcgctt ttgccgccgg cttccttgag caccatgatc tggcgcatct caaccacctg
     4321 atcgcgcagc acggccgcgc gctcgaattc cagctgttgg gccgcctgct tcatctcctt
     4381 ttccagctcg ctgatgatct tggccagttc gatcttgggc aggtcggcgg cggtcacata
     4441 gccacccttc gattcggcga gagccaactc accgcgcgcc atctcttgct gcgccgccaa
     4501 ctcgtcggtc aggacgtgta ccgccttgac gatgctccgt ggttcaatgc catgttcctt
     4561 gttgtgagcc atctggattt cgcggcggct gttggttgtg gcgatggccg cggccatcga
     4621 atcggtgatc ttgtcggcgt acatgatcac cttgccctcg acgtggcgag cggcgcggcc
     4681 gatcgtctgg gtcagcgccg tctcggaacg caggaagccc tccttatcgg cgtcgaggat
     4741 ggcgacgagc gacacctccg gcaggtcaag cccttcacgc agaaggttga tgcccacgac
     4801 cacgtcatag acgcccaggc gcagatcgcg caggatctcc gtgcgttcca gggtggccac
     4861 ctcggagtgc agataatgga ctttgatccc catctccagc agataatcgc tcaggtcttc
     4921 ggccatgcgt ttggtcagcg tcgtcaccag gacgcgctgg ccgttggcga tgcgccggtt
     4981 gacctcgccg atgaggtcat cgacctggcc gcgcaccggt cgcacctcaa cctcagggtc
     5041 gataatgccc gtcggccgga taacctgatg agctatttgt tcggagtggg ccatctcaaa
     5101 ggggccgggc gtggcgctgg tgaagatgac ctgattgagc aggccctcga actctccgaa
     5161 gttgagcggc cggttgtcca tcgcgctggg caggcggaag ccataatcga cgaggatgct
     5221 cttgcggctc tggtcgccgc cccacatgcc gcgcacctga gggatggtca tatggctttc
     5281 atcgatgacc atcaggtaat cggccgggaa gtaatcgagt aacgtccagg ggcgcgatcc
     5341 ggccggccgt tgatccaggt gacggctgta gttctcgatg ccgggggtga agcccacctc
     5401 gcgcagcatc tcgagatcgt acatgactcg ttgctcgatg cgctgggctt ccagatagcg
     5461 atcttccttc ttgtagaagg ccacgcgatc ggtcagctcg gtctcgatgt cattgatggc
     5521 tcgcgccagc ttctcctcgt cggtgatgaa ttcgcgcgcc gggtagatgg cgatttgggt
     5581 gtgttcgagc aggacttcgc cggtcagcgg atcgaactcg gtgatgcgct cgacgatatc
     5641 gccccagaac tcgacggaga tagccgtttc ggcgtaggcc gggtagatgt ggagcgtgtc
     5701 gccccgcacg cgaaaggtgc cgcgtcgcag gtcgagatcg ttgcggtcgt aatggatatc
     5761 gacgaggtgg cgcagcacgg tgttacgacg atactgcccg ccgcgctcca actccaggat
     5821 gaccttcccc caggcttcgg gattgccgat gccgtagatg cacgagactg aggcgacgat
     5881 gatgacatcc ttgcggctct tcagagtcgt catggcttgc aggcgcaggc ggtcgatctc
     5941 ctcgttgatt tgcgtttctt tctcgatgta gagatcgtgg cgggggatgt aagcctcggg
     6001 ctggtagtaa tcgtaatagc tgacgaagta ggagacggcg ttttgcggga agaagtcgcg
     6061 gaactcgcta tagagttggg cggccaatgt tttgttgtgc gccaggataa gcgtcggccg
     6121 ctgggtcttc tggatgatat tggcgatggt gaaggtcttg ccggtgccgg tggcgccgag
     6181 cagggtctga aacttgtcgc cctgatagat cccttccacc agggtgtcta tcgctgccgg
     6241 ctggtcaccc gtcgcggcga aatcggatac cagtttgaac tcagacatga ctagcctcgt
     6301 tgtcggtgtg cccgtgacat tagaacgaaa gttcttattt tatcactttg ggagatgcgg
     6361 atcaatagct ccttaggcat taaccacaac cgatgagcga tcgttgcttt gattggcgcg
     6421 caagctcata agcccgttat ttttcgacca cttcgatctc gaacagcgtg ggccacagct
     6481 tgccggtgac gaacagacgg ccgctgtcgt cgacagcgat gccgttgagc acgtcggccg
     6541 gttgggtcag tgtgccggta tccagcaagc cggtcagatc gacatagctc agcacgtcgc
     6601 ccgtctccgg tgagatgcgg gcgatgaggt cggtcagcca gatattggcc cagatttcgc
     6661 cgtcgatata ttccagctca ttgagccgat ttaccggccc ctgatcgtcg cgcacggtga
     6721 tattgccgat ttcctgaagc gtattggggt cccagaagcg aatagtggat gagccgtcgc
     6781 tgacgatgag acgtgttccg tcgtgggtga taccccaccc ctcatgaggg tagctgaagg
     6841 tttgcaaggg ttcgaatgtg tcgcgctcat aaatgaagcc ggtcaactcc tgccaggtca
     6901 actgatagat cttgccgttg aagacagtga tcccctcgcc gaaatactgg gcatccagcg
     6961 agtgcgagcg cagcaccgcg ccagtttgca gatcgacctc acgcagcgtg gactcacccc
     7021 agcgacctgt gccctcaaag aggcgaccgt cgtcgaaatc aaggccctgc gtgaaggcgt
     7081 tggggtcatg gggaaaggta ttgatgaccc gatacgtata gacggttggc ggagcggggg
     7141 taggggtagg cgcttccgtg ggtgtggggg cgggggtggg cgtcccttgg gcggcgacta
     7201 tcgccgcata ggcgacctcg gttgccgc
//
LOCUS       DAOMQG010000154         7129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4504_length_7129_cov_9.595137, whole genome shotgun sequence.
ACCESSION   DAOMQG010000154 DAOMQG010000000
VERSION     DAOMQG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_7129_cov_9.595137"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLC06_09515"
     CDS             <1..752
                     /locus_tag="PLC06_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRO90723.1"
                     /translation="VGWIDSLATGKSLGRGDVHRANYLSAGDDPQPAQTLRPRNQHLG
                     PNLFGLVPRSIVWLFMRPMFSNWGVQFVNQGKYLAGNLGGVKKYRQPHAQFHFLLDYV
                     PDWKKAYGPGGLIQYQPFIPKETAFDAFSEILTLCKRRGLPNYLTVLKRHRPDEFLMS
                     YAVDGFSLAMDFRVTDENRQRLVWLTQELDEIVVQAGGRFYLAKDSTLRPEIAQAYLG
                     QERIDQFKALKARCDPAGMLETDMWRRVFSS"
     gene            complement(759..1205)
                     /locus_tag="PLC06_09520"
     CDS             complement(759..1205)
                     /locus_tag="PLC06_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90724.1"
                     /translation="MPVANHQPGESFVVQFAWRLPDGEYLRAMFQADIVDLVPGADKY
                     VVRLSRFLAGREDDVEGQVKPLESLEGAYWDQVRGLEGRRITIAYEADDGRPLYLRLA
                     TLTGEHNFFSRYEDAAVIARGIETRMKRSQLSAAEDDPAAKDGTDQ"
     gene            complement(1285..2385)
                     /locus_tag="PLC06_09525"
     CDS             complement(1285..2385)
                     /locus_tag="PLC06_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90725.1"
                     /translation="MASVSFRHVYKRFGDVSVIKDFNLEIADKEFMVFVGPSGCGKST
                     NLRMLAGLEDVSDGDILIGDRVVNDVPPKDRDIAMVFQSYALYPHMTVYDNMAFGLKL
                     RKMPKDEIDRRVKQAADIMGLSNLLDRKPKALSGGQRQRVAVCRAIVREPSVFLMDEP
                     LSNLDAKLRVTARAEISKLHQRLGTTFIYVTHDQVEAMTMGDRIAVLADGILQQCDTP
                     RNLYNEPVNVFVAGFIGSPAMNFFNGTLVSEEGRQFIDTGDFRVRVPEDRKEAFAPYV
                     GKEIIMGIRPEHVHSPDFAPTNINGELVNATVEVVELLGHELHLYLNSGKNSFVGTVD
                     PRFGAHSGNQITVAFDMNNVQLFDKATELAIR"
     gene            complement(2570..2905)
                     /locus_tag="PLC06_09530"
     CDS             complement(2570..2905)
                     /locus_tag="PLC06_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO90726.1"
                     /translation="MSETRPVCPRCFKNKRQARDGRTPAGSQRYRCAACGCRYTPTPK
                     DQGYQEAVRFQALQLYLEGHSMREIGRILNVNHQSVANWMKDYTRFLPPDLPPDIAEL
                     ARLEGLYVL"
     gene            complement(2914..3954)
                     /locus_tag="PLC06_09535"
     CDS             complement(2914..3954)
                     /locus_tag="PLC06_09535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRO90727.1"
                     /translation="MSDSPVIDPVDSQDVVASPPGGSVESPHNEGTVLHGRYRLTNIV
                     GQGGMGNVYRAEDLRLPGRLCAIKEVTPDPYASQEMRDQSHQQFLQEASILAQLDHPN
                     LPKVSDFFSDNNRDYLIMDYVPGQDLKQWLDNARAENRKLSESQVLGWAAQIIDALSY
                     LHRQTPPVVHRDIKPSNIKLTPDNRIKLVDFGLVKLLSPDEARTITVVQGRGTALYTP
                     LEQYGGDSGHTDVRTDIYALGATFYHLLTDFPPPDAKTRFLNPRALRDPAQLNSTISP
                     HVSEAILWALEMHPDDRPQTIEQFAQALFGRGGRPHPGKSISFGEALRANWFVAIMAL
                     ALFVLALLATLT"
     assembly_gap    4085..4184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4185..4379
                     /locus_tag="PLC06_09540"
     CDS             <4185..4379
                     /locus_tag="PLC06_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO90728.1"
                     /translation="YDADGSVCGRDDIIEAVWPGTGGQGVSEQAIDALVRRLRDRLAE
                     LDEHNYIVTVRGHGFRLDNP"
     gene            complement(4451..5803)
                     /locus_tag="PLC06_09545"
     CDS             complement(4451..5803)
                     /locus_tag="PLC06_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRO90729.1"
                     /translation="MQTQITCPNCGTPYVADIYQVVDVGRQPQLKEMLLSGQLNFAVC
                     PNCGAGGRIATPLLYHDPAHDLFMIHIPQEMPMDQVRREELVGRLAQQVINQTPMEKR
                     RGYMLQPQTMLTMQSFMERVWETEGVTPDMLARQQKQVELLRTLATASPDVQDYLIKE
                     RGREIDETFFGILRAQIEATSQMEDSNLVNELLNLQAKLMTGTEVGRKLEKQQIALHA
                     LSRDAKRENGLTPKLLAQHIIANEADDSLVNAIALTGRGAMNYEFFTRLTEEVEKREK
                     AGDKTGAQRLGRVRDQLLELQKEMQEATQQVLREAQATLDEILKADNLEESIAVNMGR
                     IDDAFMHVLEARQIHAEQSGHRDEQEKLRRIQEIIITQIQGETPPEIKLLTQLVSAQS
                     KEERDQLIAGAPELISDELVKVVDMLLDQAENAGQADLAHKLEEIRPELKVRLLAGNA
                     "
     gene            complement(5832..>7129)
                     /locus_tag="PLC06_09550"
     CDS             complement(5832..>7129)
                     /locus_tag="PLC06_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO90730.1"
                     /translation="IAREADLGLTQVIRLPLSTGNVVTGLIYVFQSGNYYFVEDAPNL
                     LRSFAEQAAIAVRNAKLYHQVNQEKQRLDAILEQSADGVMILDRNLRITVFNQALSHM
                     TGWSAAEAIGCAHDDVIQWVSLKSEPDLQTALNYHWPQPNAAHLYVEGEARRHKNSTY
                     QSERDTINLGITYAPMLDANGQMTDIIASIRDLTRYREEEALQKTFISVISHELKTPV
                     SIIKGYAGTLRRPDGRWSPEILDESLAVIEEEADNLNVLIDNLLEASRLQAGTFVLEV
                     SDEVNLRAMAESVARRFLTQTAIHTIKTEFPDDFPTVIGDERRLTQVLNNLVSNAIKY
                     SPEGGQITIKGQIHPEHVTVSVCDEGIGISARDQNRIFQKFSRLDNALSRKTEGTGLG
                     LYLSRAIVEAHHGRIWLQNNSDTTPGASGTTFTFSLPRN"
BASE COUNT         1370 a         1908 c         2077 g         1674 t
ORIGIN      
        1 tcgtaggctg gatcgattcc ctggctaccg gcaaatcgct ggggcgaggt gatgtccatc
       61 gagcgaatta cctgtcggcg ggagacgatc cgcagccggc ccagacgcta cgccccagaa
      121 atcagcacct ggggccaaat ctctttggtc ttgtgccacg ctcgatcgtc tggcttttca
      181 tgcgcccaat gttcagcaat tggggcgtgc aatttgtgaa ccagggcaaa tatctggctg
      241 gcaacctggg gggcgtgaag aaatatcgcc agccccatgc acagtttcat ttcctgctcg
      301 actatgtgcc tgactggaaa aaagcatatg ggccgggcgg ccttattcaa tatcagccat
      361 tcattcccaa ggagacggcg ttcgacgcgt tctccgaaat cctgaccctt tgcaagcggc
      421 gcggcctgcc caactatctg accgtcctca agcgccaccg tccggacgaa ttcctgatga
      481 gctacgctgt cgatgggttc tctttggcca tggacttccg tgtgaccgac gaaaaccggc
      541 agcggttggt ctggctgacc caggaactgg atgaaattgt cgtgcaggcg ggcggccgtt
      601 tttatctggc caaagatagc acgttgcggc cggaaattgc tcaggcctat ctgggccagg
      661 agcgcatcga tcagtttaag gcgctaaaag cgcgttgcga tcccgcggga atgttggaga
      721 ccgatatgtg gcgccgggtt ttctccagct gagagaaatt actgatccgt cccgtctttg
      781 gctgccggat cgtcttcggc ggcgctcaat tgtgaccgct tcatacgtgt ttcgatgcca
      841 cgggcgatca ctgcggcgtc ttcgtatctg ctgaagaagt tgtgttcccc tgtcagggtg
      901 gccagccgca agtagagggg gcggccgtca tcggcctcat aggcgatcgt gatgcgtcga
      961 ccctccagac cacgcacctg atcccaatac gctccctcca ggctttccaa tggcttcact
     1021 tgcccctcga catcatcttc acggccggcc agaaatcgac tcaggcgcac gacatactta
     1081 tcggctcccg gcacgaggtc cacgatatcc gcctggaaca tggcgcgtag atactcgcca
     1141 tcgggcaatc gccatgcaaa ttggaccacg aacgattcgc cgggttgatg attggctacg
     1201 ggcatcgtga tctctggggg ttaaagaaga gggtcgccgt agagcggcga ccctcggtat
     1261 gcttattctg gtgaacgcgg gtagttaacg gatcgccagt tcagtcgcct tgtcgaatag
     1321 ctggacattg ttcatgtcga aggcaacggt gatttgattg ccgctatgag cgccgaagcg
     1381 gggatccacg gtgccgacga agctgttctt gccggagttt agatacaggt gcagttcgtg
     1441 gcccagaagc tccaccactt ccacggtcgc attcacaagc tcgccattga tattagtcgg
     1501 cgcgaaatcg ggggaatgga cgtgttcggg gcggatgccc atgattattt ccttaccgac
     1561 atagggcgcg aaggcttcct tgcgatcctc gggaacgcga acgcggaaat cacccgtgtc
     1621 gataaattga cggccttcct cgctgaccag cgttccgttg aagaagttca ttgccgggct
     1681 gccgatgaat ccggcaacga acacattgac cggctcattg tacaggttgc gtggtgtgtc
     1741 gcactgctgc aggatgccgt cggccagaac ggcgatgcga tcgcccatgg tcatggcctc
     1801 aacctgatcg tgcgtgacgt agatgaaagt cgtgcccagt ctctgatgca atttgctgat
     1861 ctccgcccgg gcggtgacgc gcagcttggc atcgaggttg gacagaggtt cgtccatgag
     1921 gaagacggac ggctcacgaa cgatggcgcg gcatacggcg acgcgctggc gctggccgcc
     1981 ggaaagcgcc ttgggcttcc gatcgagcag gttcgacaaa cccatgatat cggccgcctg
     2041 tttcacgcgc cggtcgattt cgtccttggg catcttgcgc agcttcaagc cgaaggccat
     2101 gttgtcgtag acggtcatat gtggatagag agcgtacgac tggaagacca tggcaatgtc
     2161 gcgatccttg gggggcacgt cattgaccac gcggtcgcca atcaggatgt cgccatccga
     2221 tacatcttcc agtccggcga gcatgcgcag gttggtcgat ttaccacatc ccgagggacc
     2281 gacaaatacc atgaactcct tgtcggcgat ctccagatta aagtccttga tgacgctgac
     2341 atcgccaaat cgtttgtaaa catgccggaa acttacgctt gccatagtct gctcctgttt
     2401 gtgatgaatg ccaaagaact aaagggtctc tatcttctac ggattgggtt tgcctgtgac
     2461 tatagctttg ctggcggcag cgtcaacgat atcgtctaat atcgtggtgg ctttgtctaa
     2521 tatcgtggtg gtttacgact tctcttggcg tcggttgtgg gtgaaccgtt tagagaacgt
     2581 acaggccttc gagcctggcc agttcggcga tgtcgggcgg cagatcgggc ggcagaaagc
     2641 gcgtataatc cttcatccag ttggcgacgc tctggtgatt gacattgagt atccgcccaa
     2701 tttcgcgcat ggaatgaccc tccagataga gctggagcgc ctgaaaccgc accgcttcct
     2761 gatacccctg atctttggga gtgggggtgt agcgacaacc acaagcggca caccgatagc
     2821 gttggctgcc ggccggcgtt cggccgtcac gcgcctggcg tttattcttg aagcagcgcg
     2881 ggcagacagg tcgagtttcg ctcatgatat ggctcaggtg agggtggcca gcagcgccag
     2941 aacaaataaa gccagagcca tgatggcgac gaaccagttg gctcgcagag cctcgccgaa
     3001 cgagatcgat ttgcctgggt gtgggcgacc gccgcggccg aagagcgcct gggcgaattg
     3061 ctcgatcgtt tgcggccgat cgtccgggtg catctccagt gcccagagga tagcctcaga
     3121 cacatgaggg gagatagtgg agttcagttg agccggatcg cggagcgcgc gcggattgag
     3181 gaatcgtgtt tttgcgtccg ggggcgggaa gtcggtcaga aggtgataga acgtggcccc
     3241 cagcgcatag atgtcggtgc gcacgtcggt gtggccgctg tcgcccccat attgttccag
     3301 gggcgtatac aaggctgtcc cacgtccctg cacgaccgtg atcgtccgtg cctcgtccgg
     3361 cgagaggagc tttaccagcc cgaaatcgac taacttgatc cgattatcgg gcgtcagttt
     3421 gatattgctg ggcttgatat cacggtggac gaccggcggc gtttgccggt gaaggtagct
     3481 cagtgcgtcg atgatctgcg cggcccatcc cagaacctgg ctttcgctga gtttgcggtt
     3541 ttcagcgcgt gcgttgtcca gccattgctt gaggtcctgg ccggggacgt aatccatgat
     3601 caggtagtca cggttattgt cggagaagaa atcggagact ttcggcaggt tggggtgatc
     3661 cagctgggcc aggatgctgg cttcctgcag gaattgctgg tgcgactgat cgcgcatttc
     3721 ctgggaagcg taggggtcgg gtgtcacttc cttgatggcg cacaggcggc cgggcaggcg
     3781 cagatcttcc gcccgatata cgttgcccat gccgccttgc ccgacgatat tggtcaatcg
     3841 atagcggcca tgcagcacag tcccttcatt gtggggtgac tcaacgcttc cgcccggagg
     3901 cgatgcaacc acatcctggc tatcgaccgg gtcgattact ggactatcac tcatacaatc
     3961 cattataatg caaacagtaa aagactggca tacacgatga aatggcgtcg attgccgact
     4021 tgatgaggac ttgacgtatg gatgacagac cggtcctgat ggtgagtgag ggagaactgc
     4081 ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntatgat gccgacggct
     4201 cggtctgcgg ccgcgatgac attatcgagg ctgtgtggcc ggggacgggg gggcagggcg
     4261 tttcggagca ggcaattgat gcgctggtcc gtcgcctgcg cgatcggctg gctgagctgg
     4321 acgaacacaa ctacatcgtg acggttcgcg gtcatggatt ccgcctcgat aatccctaga
     4381 aactaaaaag ccgtcatgaa gaaaaatcat ggcggctttg ttgattcagc ttattaagag
     4441 cgacagcgta tcaggcattg cctgccaata gcctgacctt taactcaggc ctaatctctt
     4501 ccagtttgtg cgccagatct gcttggcccg cgttctcggc ctggtccaat aacatatcca
     4561 ctactttcac caactcatct gaaatcagtt cgggcgcacc ggcaatgagc tgatcgcgct
     4621 cctctttgct ttgggcgctc acaagctgag tgagaagctt tatctccggc ggagtttctc
     4681 cctgaatctg ggtgatgatg atctcctgaa tacggcgcag cttttcctgc tcatcacggt
     4741 gaccgctctg ttcggcgtgg atttgccgtg cttcaagaac gtgcatgaac gcatcgtcga
     4801 tacgtcccat gttgacggcg atggattctt ccagattatc ggctttcagt atctcatcca
     4861 gcgttgcttg agcttctctc aatacttgct gggttgcctc ctgcatctcc ttttgcaact
     4921 ccagcagttg atcgcgcact cgacccaggc gttgggcgcc ggttttatcc cccgccttct
     4981 ctcgcttttc cacctcttcc gtcagccggg tgaagaactc ataattcatc gccccgcggc
     5041 cggtcagggc aattgcattc accagagaat cgtcggcctc attggcgatg atgtgctggg
     5101 caagcaattt aggggtcagg ccattctcgc gcttcgcgtc gcggctaagg gcatggaggg
     5161 cgatctgctg cttctccagt ttgcggccga cttctgtgcc ggtcatcagt tttgcttgca
     5221 agttgagcaa ctcattcacc agattgctgt cttccatctg gcttgtcgct tcaatttgag
     5281 cgcgaaggat gccgaagaat gtctcgtcga tttctcggcc gcgctcctta atcagataat
     5341 cctgcacgtc gggtgaggcg gtggccagtg tgcgcagaag ctcaacctgc ttttgttggc
     5401 gagcgagcat atccggcgtc acgccttcgg tttcccacac gcgttccatg aagctttgca
     5461 tggtcagcat cgtttgcggc tggagcatgt agccgcgccg tttttccatt ggagtctggt
     5521 taatcacctg ttgagcaagc cggcccacca actcctcgcg tcgcacctga tccatgggca
     5581 tctcctgagg gatatggatc atgaacagat cgtgcgccgg atcgtgatag aggagcggtg
     5641 tggcgattcg gcctcctgcg ccacaattag ggcagacggc gaagttgagc tggcccgaca
     5701 gcaacatctc tttcaactgc ggttggcggc ctacgtccac cacctgatag atgtctgcta
     5761 cgtagggcgt tccgcagttc gggcatgtaa tctgagtttg catgtattct cctgttgatt
     5821 aggtgacgct tctagtttcg tggaagcgag aatgtgaacg tggttccgct cgcgcctggg
     5881 gtcgtatcgc tgttgttctg gagccagatt cggccgtgat gggcctcgac aatggcacga
     5941 gacagatata accccagccc ggtgccctct gtcttgcggc tgagggcgtt gtcgagccgg
     6001 gaaaattttt gaaagattcg gttttgatcg cgggcagata tgccgatccc ttcgtcgcat
     6061 accgaaacgg tcacgtgctc aggatgaatt tggcccttga tcgtgatttg ccccccttcg
     6121 ggtgaatatt tgatggcgtt gctgacgagg ttgttgagga cctgggtcag gcgacgttcg
     6181 tcaccgatta ccgtcgggaa atcatccggg aattccgtct tgatagtgtg gatggccgtt
     6241 tgtgtcagga accggcgggc gacggattca gccatcgctc tcagattgac ctcgtcgctc
     6301 acttcgagga caaacgtccc tgcctgtaat cgcgatgcct ccagtaaatt gtcgatcagg
     6361 acgttgagat tatcggcttc ttcttcgatc accgctaatg attcatccag gatttcgggc
     6421 gaccaacggc cgtcgggccg gcgcagtgtg ccggcatacc ctttaatgat cgataccggg
     6481 gtctttagct catggctgat gacggaaatg aacgtctttt gcagcgcctc ttcttcgcgg
     6541 tagcgagtca ggtcccggat gctggcaatg atgtcggtca tttgcccatt cgcatccagc
     6601 atcggggcat aggtgatacc gagattgatc gtgtcccgct cgctctgata ggtgctgttt
     6661 ttgtggcgac gcgcctctcc ctcgacgtat agatgggctg cgttgggctg gggccaatgg
     6721 taattcaatg ccgtctgaag atccggttcg gattttagcg agacccattg aatgacgtcg
     6781 tcatgcgcgc atccgatcgc ttcggccgcc gaccaacccg tcatatggct cagcgcctgg
     6841 ttgaacaccg tgatacgcag gtttcgatcc aggatcatga ctccatcggc gctctgttcc
     6901 aggatggcat ccaggcgttg tttctcctga ttcacctgat gatagagttt ggcgttacgc
     6961 acggcgatgg ccgcttgttc agcaaagctc cgcaggaggt tgggggcatc ctcaacgaaa
     7021 taatagttgc cggactgaaa cacatagatc aagcccgtta cgacgttgcc ggtactgaga
     7081 ggcagacgga tgacctgggt cagccccagg tctgcttcac gagcgattt
//
LOCUS       DAOMQG010000155         7095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4527_length_7095_cov_10.407670, whole genome shotgun sequence.
ACCESSION   DAOMQG010000155 DAOMQG010000000
VERSION     DAOMQG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_7095_cov_10.407670"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLC06_09555"
     CDS             <1..339
                     /locus_tag="PLC06_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90731.1"
                     /translation="SKLTRSTEPYQTTVVGVYSSQPAFLGNSAGEEQDGRVPLALVGV
                     VPVKVSAENGPIHPGDLLVASATVGHAMRAEPVVVDGVTFYPSGVVIGKALGSLDEGT
                     GMIDVLVMLQ"
     gene            350..637
                     /locus_tag="PLC06_09560"
     CDS             350..637
                     /locus_tag="PLC06_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90732.1"
                     /translation="MNRRQIQFLALALLIVMLAIGGVVLASSGNGFAVGWFRLGPGGR
                     SFCADGYSLTSAIGQVAVAESNGDSYSLISGVRLEAADCTSISLPVILRQH"
     gene            complement(758..880)
                     /locus_tag="PLC06_09565"
     CDS             complement(758..880)
                     /locus_tag="PLC06_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90733.1"
                     /translation="MQASRFQLDRLLDPENTAVTLDSLERLARAVGKRLGVELA"
     gene            1238..2200
                     /locus_tag="PLC06_09570"
     CDS             1238..2200
                     /locus_tag="PLC06_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRO90734.1"
                     /translation="MRIGVLTVLFQNLTFEEALDKAAAAGVSAVEIGAGGFPGRHHCP
                     VDDLLAGVGERRAYLAALESRGLLLSALSVHNNPLHPDAAVAKTADEELRKAIRLAQA
                     LGVPVVNTFSGLPGGASGDKSPNWVTSPWPPEFLAMLDYQWNEVAIPYWREMEQFAAD
                     HGVKIAFEMHPNMLVYNVETMLRLRAATGPALGCNFDPSHLWWNGVDAAAAIRKLGDA
                     IFHVHGKDCFVDRSNIRVNGCNDHKPYDRILDRAWTFRSIGYGHDVAVWKEIVSSLRL
                     VGYDYVISIEHEDALMSNDEGLAKGIALLKDVVINDPPGEMFWA"
     gene            2447..2806
                     /locus_tag="PLC06_09575"
     CDS             2447..2806
                     /locus_tag="PLC06_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRO90735.1"
                     /translation="MRTTHLLENLVYHDEHPYAQPLYVSEDGRVIRFMMKPGQSIEEH
                     NAPSSPFYVVVVEGHGMFSGADGAEREFGPNDVLIFDKGENHAVRALDEELVFIGFLH
                     GVEGTRPGKIGGLLADE"
     gene            3039..3290
                     /locus_tag="PLC06_09580"
     CDS             3039..3290
                     /locus_tag="PLC06_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRO90736.1"
                     /translation="MIPTTTVRISRKTRDIIQEMAQTSGRSMQDIMEEAIAAYQGRQL
                     LEATNAAYAVLKDNPGTWRELQEERAEWDAALNDGLEEL"
     gene            3291..3638
                     /locus_tag="PLC06_09585"
     CDS             3291..3638
                     /locus_tag="PLC06_09585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRO90737.1"
                     /translation="MAASPNRGEVWLADLNPTQGHEQAGKRPVLVVSTDVFNHGPADL
                     IFVLPLTRTNRGIPIHVPLRPPEGGVSAPSYILCDALRSISKDRLDSKAWGKVSPATM
                     ERVEEILRLLMEL"
     gene            complement(3656..4891)
                     /locus_tag="PLC06_09590"
     CDS             complement(3656..4891)
                     /locus_tag="PLC06_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRO90738.1"
                     /translation="MDNHPVPELTLTLTDMAHGGLALGRDKSGRAIFVPFAIPDETVR
                     VRVPEDKRGFARAELLEVLKPSPDRIAPRCRHFGICGNCHLQHMSYAAQLQAKEAAVR
                     DQLTRVGGLKNPPLRPILPAPEPYGYRAETALFPAEGGGLGYWSPVERGIFRVEECPI
                     LQPALESALPDLDVELPGLRRLSLRLGDDEELLAALETEDVEPPELAVDFPVSVAIVL
                     PDRTAASLIGDPYLMRTLAGREYRFSPGVAWPANPAAAELLAGVVLELAEITADDVVL
                     ESPGGAGWLTAALAQRAASVIAVEPNPDAVADAAENLDAFDNVSLYEGTEEEVFPALE
                     TQPDVVVLRPADGLSPAAFRLLERLHPRRRVVVTGEVGALAKDAKRLAKMGYRPLAFQ
                     PVDLSPQAFQVEIVSLWRK"
     gene            complement(4978..5745)
                     /gene="cofE"
                     /locus_tag="PLC06_09595"
     CDS             complement(4978..5745)
                     /gene="cofE"
                     /locus_tag="PLC06_09595"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HRO90739.1"
                     /translation="MNTNHLTLFALPGIPDVRPGDDVGRLLLAALDQAGVTLQDGDVV
                     AIAQKIISKAEGRLVDLAGVVPGERARELARQTDKDPRLVELILRESDEVSRVRPGVI
                     IVRHRLGFTSANAGIDRSNVGPDGEERVLLLPVDPDASAHRIRDLFITERNVALGVVI
                     TDSHGRPFRLGTVGVAIGVAGLPALWDRRGEADRYGYRLQHTDVGVADEIAAAAGLLM
                     GQAAEGQPAVLLRGLRLPFVDGRASDLVRPKDLDLYR"
     gene            complement(5876..6538)
                     /gene="npdG"
                     /locus_tag="PLC06_09600"
     CDS             complement(5876..6538)
                     /gene="npdG"
                     /locus_tag="PLC06_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008384977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HRO90740.1"
                     /translation="MKIAILGGTGKEGSGLAYRWAAAGHEVIIGSRTAEKGEAAAEEM
                     QSELPQATIAGADNAAAAAAAELVVLSVPYDAQEKTLADVHDAVQGKLLITVVAPLGQ
                     PKARAWRLPSGLSAAEEAQQQLGEGVTVVAAFQNISASHLRDLDHDLDCDVLICGEKA
                     ADKEVAAGLCRGAGMRGINAGALANAAVAEGLTAVLLGINIRNKISGAGIRITGLPDD
                     GF"
     gene            complement(6790..>7095)
                     /locus_tag="PLC06_09605"
     CDS             complement(6790..>7095)
                     /locus_tag="PLC06_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRO90741.1"
                     /translation="IYNNAALARVFWPDLPLGRLAVGAAADVILVDYHAITPLSAGNL
                     PWHIIFGFESSMVTTTIVAGQVLMRDRQLLTLDEAAITARSRELAAKVWERYNALDG"
BASE COUNT         1310 a         2174 c         2266 g         1345 t
ORIGIN      
        1 agtaagctga cgcgcagcac agaaccgtat cagacgacgg tcgtgggcgt ctattccagc
       61 caacccgcct ttctgggcaa cagcgccggc gaagagcagg acggccgcgt gccgctggcg
      121 ctcgtcggcg tcgtgcccgt caaggttagc gccgagaatg gcccgattca tcccggcgac
      181 ctgctggttg cgtccgctac cgtggggcac gccatgcgcg ccgaaccagt ggtcgtcgat
      241 ggcgttactt tctatcccag cggcgtggtg atcggcaagg cgttgggatc gctcgacgaa
      301 ggcacgggca tgatcgacgt gcttgtcatg ttgcagtaag gaggggatga tgaatcgccg
      361 acagattcaa tttcttgctc tggcgctgct catcgtgatg ctcgccattg gaggcgttgt
      421 tcttgcctcg tcgggcaacg gctttgcagt cggctggttt cgattaggtc cggggggacg
      481 ctcattctgt gccgatggat atagcttgac aagcgcaatc ggccaagtcg cggttgcaga
      541 atccaatggc gacagctatt cgttgattag tggggttcgg ttggaagcgg ccgattgcac
      601 atccatttca ttacctgtca tcctgaggca gcattgaagg caattattgg gcaaatagcc
      661 gcctggcccg cgtctccagc ataattagta tctgcaaggc tagattgctc ccacttgttg
      721 cgaagtggct cggccagcct tgtccaatat cacttgtcta cgccaactct acccccaacc
      781 gcttccccac cgcccgcgcc aatcgctcca gcgaatccag cgtgacggcc gtgttctccg
      841 ggtcgagcag gcggtcgagt tggaagcggc tggcctgcat ccactcgata aagcaacaaa
      901 ctttccgtaa aagttaaaaa cctcggtcat cacaagatca tcgccaccaa tttacagaaa
      961 gaaagttgca ctatctcatc tcggtcagga aaccggagtt acggtgtacc gccggattct
     1021 tatccggcgc cgcgactgag gagaatcatc tccggttagg aaaccggagt tacatgcatg
     1081 ttaggccgct tttgcataat tggaacgggc tgctgaaaaa ccctgcctaa tccttccata
     1141 aggtggcaca atcggccgat cgccgtatga tcggccccaa cccattcaac ccatcggggc
     1201 ggcccggctt gccgtcccat tgatcggagg attatccatg cgcattggtg tactaaccgt
     1261 cctgttccaa aacctcacct ttgaagaggc actcgacaag gcggcggccg cgggcgtgtc
     1321 ggccgtcgag atcggcgcgg gcggctttcc cggccgccat cattgccccg tcgacgacct
     1381 gctggccggc gtgggcgagc ggcgggccta tctggcggct ctcgaatcgc gcgggctgct
     1441 gctcagcgcg ctcagcgtcc acaacaaccc gctccacccc gacgcggccg tggccaagac
     1501 ggccgacgag gaactacgca aggcgatccg tctggcgcag gctctcggcg tgccggtcgt
     1561 caacaccttt tccggactgc cggggggcgc gtcgggcgac aaatcgccca actgggtgac
     1621 cagcccctgg ccgccggagt ttctggcgat gctcgactat cagtggaacg aggtcgccat
     1681 cccctattgg cgcgagatgg agcagttcgc cgccgaccac ggcgtgaaga tcgcctttga
     1741 gatgcacccc aatatgctgg tctacaacgt ggagacgatg ctgcggttgc gcgcggcgac
     1801 cggcccggcg ttgggctgca acttcgaccc cagccatctg tggtggaacg gcgtcgacgc
     1861 ggcggcggcc atccgcaagc tgggcgacgc catcttccac gtccacggca aggattgctt
     1921 cgtggatcgg agcaacatcc gcgtcaacgg ttgcaacgat cacaaaccct acgatcgaat
     1981 cctcgaccgc gcctggacgt tccgcagcat cggctacggt cacgatgtgg ccgtgtggaa
     2041 agagatcgtc agttcgctgc gactggtggg ctacgactac gtcatctcca tcgagcatga
     2101 ggacgcgctg atgagcaacg acgaagggct ggccaagggg atcgcgctgc tgaaggatgt
     2161 ggtcatcaac gacccgccgg gcgagatgtt ctgggcataa aggaatcgcg gagattcgag
     2221 ggggtggttg cggggcaacg ccgggatttg attccggtca taatacctat cgtcaagggt
     2281 ctcttcatcc gttacccttg aaagttgcct gtccaatact tgactatcga tggcgcgatg
     2341 gccaactgga ttgaagcagg tcgccacgcg ccccgaccca tgtcctcgcc gccggctctt
     2401 tgtttgcggc cgcctcgtgg actatcacaa aaggagataa tgtaagatgc gtacaaccca
     2461 tttgctggaa aatctggtat atcacgacga acacccctat gcccaaccgc tgtatgtcag
     2521 cgaggacggg cgggtcatcc gcttcatgat gaagcctggc cagagtatcg aggagcacaa
     2581 cgcgcccagc tcgccgttct acgtggtggt ggtggagggg cacggcatgt tctccggggc
     2641 cgacggcgcc gagcgagagt ttgggccgaa tgacgtcctc atcttcgaca agggcgagaa
     2701 ccacgccgtg cgcgcactgg acgaggagct tgtcttcatc ggctttctcc acggcgtgga
     2761 aggcacgcgc ccgggcaaga tcggcggctt gctggcggac gaatagcggt cgccgggctg
     2821 cggctgttcg gttgtgtcgg ggtcaacttc aatgatgagt tggagcaggc gatggacgat
     2881 ctgcgtcaaa gagacctcga cgttggcaag cacgtcgtcg attgagccga gtcaattgtc
     2941 ggtttgcagg gcattcgctc ttcttgatcg tcgaatagat agcccgtata atatactgta
     3001 ttgtagatga tattgtagat taatgggtga cagataatat gatcccgacc acaaccgtcc
     3061 gaatttcacg caaaacacgg gatattatcc aggagatggc ccaaacttcc ggccggtcga
     3121 tgcaggatat catggaagag gcgatcgccg cctatcaggg ccggcagctc cttgaagcga
     3181 ccaacgccgc ctacgccgtc ttgaaagata accccggaac ctggcgagag ctgcaggaag
     3241 aaagggcgga atgggacgca gcgcttaatg atggtcttga ggagctttag atggccgctt
     3301 cgcccaatcg gggagaggtt tggctcgcgg atctgaatcc gacacaaggc catgagcagg
     3361 ccggcaagcg tccggttctt gtcgtctcca cagatgtatt caatcatggc ccggcagatc
     3421 tcatcttcgt tttgccactg acgcgcacca atcgaggtat ccccatccat gtcccgctgc
     3481 ggccgccgga agggggcgtt tcagccccaa gctacatcct gtgcgacgca ttgcggtcta
     3541 tttccaagga tcggttggac tcaaaagcct gggggaaggt ttcgcccgca acgatggaga
     3601 gggttgaaga gatactacga ttgctgatgg aattgtaact ccggtttcgt gaatctcatt
     3661 tccgccagag agagacgatc tcaacctgga acgcctgcgg cgacagatcc acgggctgga
     3721 aagccagagg ccgataaccc atcttcgcca atcgcttggc gtccttggcc agcgctccaa
     3781 cctcgccggt cacgacaacg cgccgccggg gatgcagccg ctccagcaat ctgaaggccg
     3841 ccggcgacag cccatccgcg ggccgcagca cgacgacatc gggctgcgtc tccagcgcgg
     3901 ggaacacttc ttcttccgtg ccctcataga gggacacatt gtcgaaagcg tcgaggttct
     3961 cggccgcgtc ggccacagcg tcggggtttg gctccacggc gatgaccgac gcggctcgct
     4021 gggccaacgc cgccgtcagc caaccggccc cgccggggct ttcaagcacg acatcatccg
     4081 ccgtaatctc ggccagttcc aggacgaccc cggccagcag ttcggccgcg gccgggttgg
     4141 cgggccaggc cacaccgggg gaaaagcgat actcccgtcc ggcaagggtg cgcatcagat
     4201 acgggtcgcc gatgagggac gcggccgtgc gatcgggcag gacgatggcc acggagacag
     4261 ggaaatcgac ggccagttcc ggcggctcca cgtcctcggt ttccagcgcg gccagcagct
     4321 cctcgtcgtc gcccagacgc agcgacaacc gccgcaggcc gggcagctcc acgtccagat
     4381 cgggcagggc cgactccagc gccggttgca ggatggggca ctcctcgaca cggaagatgc
     4441 ctcgctcgac cggcgaccag tagcccagcc cacccccttc ggccgggaag agcgccgtct
     4501 ccgcgcgata gccgtagggt tccggcgcgg gcaggatcgg ccgcagtggc gggttcttca
     4561 ggccgcccac gcgggtcaat tgatcgcgga cggccgcttc cttggcttgc agttgggcgg
     4621 catagctcat gtgctggagg tggcagttgc cgcaaatgcc aaaatgacgg cagcgcggcg
     4681 ctatgcggtc gggcgatggc ttcagcactt ccagcaactc ggcgcgggca aagccgcgtt
     4741 tgtcttcggg tacgcggacg cgcaccgtct cgtcggggat ggcgaagggg acgaagatgg
     4801 cgcggccgct cttgtcgcgg ccgagggcca ggccgccgtg ggccatgtcg gtgagggtga
     4861 gggtgagttc gggcacggga tgattatcca cgaatttgga ttcctgggaa gacgatcgac
     4921 ccaccgaccc catgcactgc ttcttgccgc ggtcggcggt cagcggccgg ccgtcgttca
     4981 gcggtagagg tcgaggtctt tcggccgcac cagatcgctc gctcgtccat ccacaaacgg
     5041 caggcgcaac ccgcgcagca gcacggccgg ttgtccttcg gccgcctggc ccatgagcag
     5101 cccggcggca gcggcaatct catcggccac gcccacatcg gtgtgctgca ggcgatagcc
     5161 atagcggtcg gcctcgccgc gacggtccca cagggcgggc agcccggcca cgccgatggc
     5221 cacgccgacc gtgcccagcc ggaaggggcg gccgtggctg tcggtgatga ccacccccag
     5281 cgccacgttc cgctcggtga tgaacagatc ccggatgcgg tgggccgagg catcgggatc
     5341 aaccggcagc agcaggaccc gctcctcgcc gtccggtccc acgttggagc ggtcgatgcc
     5401 ggcgttggcg ctggtgaagc cgaggcggtg gcgaacgatg atcacccccg gccggacgcg
     5461 cgacacttcg tcgctctcgc gcaggattag ctccacgagg cgcgggtcct tgtcggtttg
     5521 ccgggccagc tcgcgggcgc gttcgcccgg tacaacgccg gccagatcga ccagccgccc
     5581 ctcggccttg ctgatgatct tctgggcgat agcgactaca tcgccgtctt gcagcgtcac
     5641 tccggcctgg tcaagcgcgg ccagcagcaa gcggccgacg tcgtcgcccg gccggacgtc
     5701 ggggatgccg ggcagggcga agagggtcag atggttggtg ttcatggggg tacccagtga
     5761 tcagtgggtc agtagatcag tagatcagta gatcagtggg tcagtgggtc agtactgaat
     5821 actgtcctgc tgaatactga tctactgacc tgctgtttta ctgatgaata ctgtcctaaa
     5881 acccgtcatc cggcaggccg gtgatgcgga tgcccgcgcc gctgatcttg ttgcggatgt
     5941 tgatgcccag caacacggcc gtcaggccct cggcgacggc ggcattggcc agcgcgccgg
     6001 cgttgatgcc gcgcatgccc gcgccgcgac acagcccggc ggcgacttcc ttgtcggccg
     6061 ctttctcgcc gcagatgagc acgtcgcagt cgaggtcgtg atcgaggtcg cgcaggtgcg
     6121 aggcggagat gttctggaaa gcggccacga cggtgacgcc ttcgcccagt tgctgctggg
     6181 cttcctcggc cgccgacagg ccgctcggca ggcgccaggc gcgagccttg ggttggccca
     6241 gcggggcgac gacggtgatg agcagcttgc cctgcacggc gtcgtggacg tcggccaggg
     6301 ttttttcctg cgcgtcatag ggcacggaga gaacgaccag ctcggcggcg gcggcggcgg
     6361 cggcgttatc cgcgccggcg atcgtcgcct gcggcaactc cgactgcatc tcctcggccg
     6421 cagcctcgcc cttctcggcc gtgcgcgagc cgatgatgac ctcatgcccg gcggccgccc
     6481 accggtaagc caacccggaa ccttccttgc cggttccacc cagtattgcg atcttcatat
     6541 gtgattatcc ttgtttgcag gttagtgttc agtaagacag caggtcagtg ttcagtggga
     6601 cagtaggtca gtgttcagta ggacagtagg tcagtgttca gtaggacagt aggtcagtag
     6661 gtcagtgggt cggtatcaat cagccagccg gttttttggc tcgtgagatt tttcttgcca
     6721 acctactgat tactgctcca ctgaatactg atctactgat tactgcctgc cggccactcc
     6781 ccaacctttt tatccgtcaa gcgcgttata ccgctcccac acctttgccg ccagttcgcg
     6841 gctgcgggcg gtgatggccg cctcgtccag cgtcagcagt tgccggtcac gcatgagcac
     6901 ctggccggcg acgatggtcg tcgtcaccat gctgctctcg aagccgaaga tgatgtgcca
     6961 gggcagattg ccggcgctta gcggcgtgat ggcgtggtag tcgaccagga tgacgtcggc
     7021 cgccgcgccc acggccagtc gccccagggg cagatcgggc cagaagacgc gggccagcgc
     7081 ggcgttgtta tagat
//
LOCUS       DAOMQG010000156         7041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4593_length_7041_cov_11.216147, whole genome shotgun sequence.
ACCESSION   DAOMQG010000156 DAOMQG010000000
VERSION     DAOMQG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4593_length_7041_cov_11.216147"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..298)
                     /locus_tag="PLC06_09610"
     CDS             complement(83..298)
                     /locus_tag="PLC06_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HRO90742.1"
                     /translation="MAAYGINTGYLLIQCVPVLLWLLLSLFVLFRLREQPMSETAKAI
                     WAVFIVVVPFFGALAFLIVRQGNRADK"
     gene            complement(305..808)
                     /locus_tag="PLC06_09615"
     CDS             complement(305..808)
                     /locus_tag="PLC06_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO90743.1"
                     /translation="MPHPLIDQLRFTRSEFIRAAKGVSDDDSRQRFLPMNCLAWNVGH
                     LAWQEQRYFLYYGQGLMLLPEIEREFAYGAPGSTPSLKEMLAAWKTITAAADPWLDTL
                     TSAKLQENAISKGKPITRNGNLLQRVIYHYWYHTGENMAIRQLLGHGRLPVFVGNIDD
                     KAPYRPE"
     gene            976..1233
                     /locus_tag="PLC06_09620"
     CDS             976..1233
                     /locus_tag="PLC06_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRO90744.1"
                     /translation="MSKRQKILARLLSGSKNITFLEFISLLDGYGFELDRIRGSHQIY
                     THPKVSELIAIQPRHDGKAKPYQLRQFLKLVEAYNLELNED"
     gene            1236..1457
                     /locus_tag="PLC06_09625"
     CDS             1236..1457
                     /locus_tag="PLC06_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRO90745.1"
                     /translation="MKDYHINIFWSEEDGEYIADVPDLKYCSASGATPNEALREVLEA
                     KLLWLEAAAEKGLPIPEPRYRPAIYQLSY"
     gene            complement(1472..2260)
                     /gene="rlmB"
                     /locus_tag="PLC06_09630"
     CDS             complement(1472..2260)
                     /gene="rlmB"
                     /locus_tag="PLC06_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRO90746.1"
                     /translation="MAPSGNSAEILTRRHPVLEALRAGRRTIYRLHVERGGRADDREV
                     RAILDAARAAKVPVVNDDRQTIDRLAQRAGADAKHQGVLLEVGPYPYADVDEMLGLAE
                     RRGERPLLLLLDLLHGPQNIGTLLRTAEACGVHGVILQDRRAPEITPAVVQYAAGATE
                     HLLIAKVTNLVQTMRSLKEAGVWLAGMDMDEDAPELGRIDLDMPLGIVVGHEGEGLRR
                     LVHERCDFIVRLPMRGQVESLNAAVAGSILLYAAWQARGFERRD"
     gene            complement(2235..3695)
                     /gene="cysS"
                     /locus_tag="PLC06_09635"
     CDS             complement(2235..3695)
                     /gene="cysS"
                     /locus_tag="PLC06_09635"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRO90747.1"
                     /translation="MSLRIYNVLSRQKEEFVPLTPGRVNIYVCGPTVYDFSHIGHAKT
                     YVSFDVVVRYLRFLGYDVLYVQNITDVGHMLDTGEDRILRKARQSSALPMQVVETYAR
                     AYFEDMDALGVRRPDISPRASGHIPEQIAMIEQLIESGHAYVMDGSVYFNVASFPEYG
                     KLSGRVVEELEEGARIAVREDKRHPADFALWKKAEPEHILRWHSPWGDGFPGWHIECS
                     AMAAKYLGDTFDIHGGGIDNIFPHNECEIAQSEAAHGEPFARYWMLTGSLTLDGVKMS
                     KSLGNSLTIKDALTRWRSEAIRYFILSSQYSNPIDFSDEAVEAAYKGWQRIWGAVSLV
                     REQLRRPDRSAEDLSGLSEEAASMLAAAREQFIERMNDDFNAPGALAVLQDLTRQVNT
                     LINEGGPQTSGTLVAIDALYRELGGDVLGIVPEHEEGGGADAAREDGLVRLLIDLRNQ
                     ARANKEWATADKIRNQLKELGVVLEDRADGTIWKLG"
     gene            complement(3757..4833)
                     /locus_tag="PLC06_09640"
     CDS             complement(3757..4833)
                     /locus_tag="PLC06_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRO90748.1"
                     /translation="MGPSRVEFISRLIGAVVLATALSIVGVRIANMLNLDPMRYGIAM
                     GVGGGVIGAVLGFLFTPHLTIRPISHIRTALSAMPLERLVALLIGVFIGLIAAALFAI
                     PLSQLPSPLKEILPLATALGFCYLGAVLMMNRYRDLHEMAGGFRWGRGGNKAVAAVEE
                     HEDTNVILLDTSVIIDGRILDIGRTGFIRQTLLVPNFVLKELQHIADSADSLRRNRGR
                     RGLDILSMLQNESPVPVRITDMDAPQTRDADSKLVVLARRLECPIMTNDYNLNKVAEL
                     QGITVLNINDLANAVKSSLLPGEELRVMIIQEGKEYGQGVGYLDDGTMVVVEDGQRAL
                     NRTENVVVTKVLQTTAGRMIFAKL"
     gene            complement(5181..6887)
                     /locus_tag="PLC06_09645"
     CDS             complement(5181..6887)
                     /locus_tag="PLC06_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO90749.1"
                     /translation="MGPNAPLGYIVGGALKSGLRVRLTVAADTVQEGGFVVCETPRYR
                     YYGLITDLQLGSTDPRFADEKSDRLPAAIQGALYGKTLYTTLEMYPTLLLDLGPELTS
                     PERAEWQARVDMGEENPGPKPVKTVPAHHAPVRPADAGDVAAIFGEEGGVNFVIGHTI
                     EQGYPVCLDLERFVKRSSGIFGATGTGKSFLTRMVLAGLMKTDVAAALVFDMHNEYGF
                     DDRDSDRGEQVRGLKAMFGEKVQIAALGKGTRVGDNSPDFTLELALGDFNADDILLIA
                     EALDLNDTAAVTLSALERVFEGRWFSEFMKMKPGAVIEDDNGKKIPAPDSVAHWANGH
                     NIHPTAAESLHRKLEPIFHKPYVVNKPAVDAVKAISDQLEHGRHIILSFGVHDNELDY
                     IIVSNILTRRIRKAWVDKTEHAKLSNVGAPRPLVIVVEEAHKLLSPGVSRQTAFGTIA
                     RELRKYNVTLLIVDQRPSGIDDEVMSQLGTRVTGWLGDEDDIRAVLSGLAGRDQLRGM
                     LARLQEKEEVLLLGWGVKMPIPVRSRRYDKRFYDEMRGRAGSSAPERPYGRQEMNDEL
                     FG"
BASE COUNT         1435 a         2151 c         2069 g         1386 t
ORIGIN      
        1 tgatgatgag cgtcggcggg cggacggcgg aagagttgcc tctatcgctg ctgttggcgc
       61 tgcaaacggt gcagcgcgcc cttcatttgt ccgcgcggtt gccctgccgc acgatcagga
      121 aagccaacgc gccgaaaaac ggcacgacga cgatgaagac ggcccagatc gctttggccg
      181 tctcagacat tggttgttcg cgcaaccgga aaaggacaaa caaggagagc agcagccaaa
      241 gcagcacggg gacgcactgg attagcaaat agccggtatt gatgccgtaa gcagccatcg
      301 gttttcactc cggacgatag ggcgctttat cgtcgatatt gcccacaaac accggcagcc
      361 gtccgtggcc cagcagttgc cggatggcca tattctcgcc ggtgtgatac cagtagtgat
      421 agatcacccg ctgcaggaga tttccgttac gggtgatcgg cttgccctta ctaatggcgt
      481 tctcctgtag cttggctgag gtcagcgtat cgagccaagg gtcggcggcc gcggtgatcg
      541 tcttccaggc ggccagcatc tccttgagcg acggcgtact ccccggcgcg ccgtaggcga
      601 attcccgttc gatctccggc agcagcatca gcccctggcc gtagtagagg aagtagcgct
      661 gttcctgcca ggccagatgg ccgacgttcc aggccaggca gttcatgggc aggaagcgct
      721 gccgggagtc gtcatcgctg acgcccttgg cggcgcggat gaactcgctg cgggtgaaac
      781 gtagttgatc gatgagtggg tgaggcataa tcctggagga tcctcttact tgagccgcat
      841 acttgccccg taccattgaa tcaaaatcct cgcgagaata ttcgggacgt aattcatcag
      901 catctccgct cacgttctcc atatatacct attatacggt ataattccaa ctcgtaagaa
      961 accggggtgg aatgcatgtc taagcgccaa aagattcttg caaggctact aagcggatcg
     1021 aagaacatca cttttctgga atttatttca ctattagacg gatatggttt cgagttagat
     1081 cggatacgag gcagtcacca aatctacact catccaaaag tatccgaatt gatcgccatc
     1141 cagccgaggc atgacggaaa agcaaaaccg taccaactac gccaattctt gaaattggtc
     1201 gaagcgtata acctggaact taacgaggat tgacgatgaa agactatcac atcaatatct
     1261 tctggagtga agaagacggc gaatacatcg ccgacgtgcc cgacctgaaa tactgctctg
     1321 catcgggcgc tacacccaat gaagcgctgc gagaggtgct ggaagccaag ttactatggc
     1381 ttgaagctgc tgctgaaaag gggttgccaa ttcccgaacc gcgttatcga ccggcgatct
     1441 atcagctaag ttattagcag gtacggcgca cttaatcccg acgctcaaac ccccgcgcct
     1501 gccaggccgc atagagcaga atcgacccgg ccacggccgc attgagcgat tccacttgcc
     1561 cgcgcatcgg caggcgaacg atgaaatcac agcgctcatg cacgaggcgg cgcagcccct
     1621 cgccctcatg cccgacgacg atacccagcg gcatgtcgag atcgatccgg cctaattccg
     1681 gcgcgtcctc atccatatcc atacccgcca gccacacgcc cgcctctttc agcgagcgca
     1741 tcgtctgcac caggttggtg accttggcga tgagcagatg ctcggtagcg ccggcggcgt
     1801 actggacaac cgccggggtg atctccggcg cgcggcgatc ctgcaggatg acgccgtgga
     1861 cgccgcaagc ctcggccgtg cgcagcagcg tgccgatgtt ctgcggcccg tggagcaggt
     1921 cgagcagcag cagcaggggc ctctcaccgc ggcgctcggc cagacccagc atctcgtcca
     1981 cgtcggcgta gggatagggg ccgacttcca gcagcacgcc ctggtgcttg gcatcggcgc
     2041 cggcgcgctg cgccagccgg tcgatcgtct gccggtcgtc gttgaccacc ggcactttgg
     2101 ctgcgcgggc cgcgtcgaga atcgcccgta cctcgcggtc atcagcgcgg ccgccgcgtt
     2161 ccacgtggag ccggtaaatg gttcgtcgcc cggcgcgcaa cgcctccagg acggggtggc
     2221 ggcgtgtcag tatctcagcc gagtttccag atggtgccat cagctctatc ttccagaacg
     2281 acgcccagct ctttcaactg attgcggatc ttgtcggccg tcgcccactc cttgttggcc
     2341 cgcgcctggt tgcgcagatc gataagcaaa cgcaccagac catcctcgcg cgctgcgtcc
     2401 gcgccgccgc cctcttcgtg ttccggcacg atacccagca cgtcgccgcc caactcgcgg
     2461 tacagcgcgt cgatcgctac cagcgtaccg ctcgtctgcg ggccgccttc gttgatcagc
     2521 gtgttcacct ggcgcgtcaa atcctgcagc acggccagcg cgccgggggc gttgaaatcg
     2581 tcattcatgc gctcgatgaa ctgctcgcgg gcggccgcca gcatggacgc ggcttcttcg
     2641 gagagacctg acaggtcttc cgcagacctg tcaggtctgc gcagttgctc gcgcaccaga
     2701 ctgaccgcgc cccagatgcg ctgccagccc ttgtaggccg cttcgaccgc ctcgtcggag
     2761 aagtcgatcg ggttgctgta ttggctggac agaatgaaat agcggatcgc ttcggagcgc
     2821 cagcgcgtca gggcatcctt gatcgtcagc gaattaccca ggctcttgct catcttgacc
     2881 ccgtcgagcg tcagcgagcc ggtcagcatc cagtagcggg cgaaaggctc gccgtgggcg
     2941 gcctcgctct gggcgatctc gcattcattg tgggggaaga tattgtcgat gccgccgcca
     3001 tggatatcga acgtgtcgcc cagatatttg gcggccatgg ccgagcattc gatgtgccag
     3061 ccggggaaac cgtcgcccca cggactgtgc cagcgcagga tatgctccgg ctcggctttc
     3121 ttccacagag cgaaatcggc cgggtggcgc ttgtcttcgc gcacggcgat gcgcgcgcct
     3181 tcttccagtt cttccacgac gcggccgctg agcttgccgt actccgggaa tgaggcgacg
     3241 ttgaagtaga ccgagccgtc catgacgtag gcgtgtccgc tctcgataag ctgctcgatc
     3301 atggcgatct gttcggggat atggccgctg gcgcgcgggc tgatgtccgg ccggcgcacg
     3361 cccagggcgt ccatatcctc gaagtaggcg cgggcgtagg tctccacgac ctgcatgggc
     3421 agggccgaag attggcgggc ctttctcagg atgcggtctt cgccggtgtc gagcatgtgg
     3481 ccgacgtcgg tgatattctg cacatagagc acatcatagc cgagaaagcg caggtaacgc
     3541 acgacgacgt cgaagctgac gtaggtcttg gcgtggccga tatgggaaaa gtcataaact
     3601 gtcgggccgc aaacgtagat gttgaccctg cccggcgtca gcggcacgaa ctcttctttt
     3661 tgccgcgata agacgttata gattctgagc gacatacttc actccatctc atttagaaac
     3721 cgagtttttt cgaaaaaact cggtttctaa ctgtatttat aatttggcga aaatcatccg
     3781 cccggccgtc gtctgcagaa ccttggtgac gacgacattt tcagtgcggt tcaatgctcg
     3841 ttgcccatct tcaacgacca ccatcgtacc gtcgtccaga tagccaacgc cctgcccata
     3901 ctccttgcct tcctggatga tcatcacccg cagctcctcg ccgggcagga gcgacgactt
     3961 cacggcgttg gccaggtcat tgatgttgag cacggtgatg ccctgcaact cggccacctt
     4021 gttgaggttg tagtcgttgg tcatgatcgg acattccaga cggcgggcca gcacgacgag
     4081 cttgctgtcg gcgtcacgcg tttggggcgc gtccatgtcg gtgatgcgta ccggcacagg
     4141 cgattcgttc tggagcatgc tcaggatgtc aaggccgcgc cggccgcggt tgcggcgcag
     4201 gctgtcggcg ctgtcggcga tgtgctgcag ctccttgagc acgaagttgg gcaccagcaa
     4261 tgtctgacga atgaacccgg tgcggccgat gtcgaggatg cggccgtcga tgatgacgct
     4321 cgtatcgagc aggatgacgt tcgtgtcctc atgctcctca accgccgcta cagccttgtt
     4381 cccgccgcgc ccccagcgga agccgccggc catttcatgc aggtcgcggt agcggttcat
     4441 catcagcacg gcgccgagat agcaaaagcc cagcgcggtt gccagcggca agatctcctt
     4501 cagcggcgac ggcaactgtg acaggggaat ggcgaagagg gccgcggcga tgaggccgat
     4561 gaagacgccg atgagcagcg cgaccagtcg ttccagcggc atggccgaca aagccgtgcg
     4621 gatgtggctg atcggccgaa tagtcaggtg aggcgtgaag agaaacccca gcaccgcgcc
     4681 gattaccccg ccgccgacgc ccatagcgat gccatagcgc atcgggtcga gattgagcat
     4741 gttggcgatt cggacgccga cgatagacag cgccgtcgcc aggacgaccg ctcctatcaa
     4801 tcttgagatg aattcaaccc gtgatggtcc catgatgacc tcaaataata atgaatgata
     4861 acgaacgaat aaaataaaaa tggtgaatgc ttttcatgca ttcaccattc gataatcgga
     4921 taacgcttca gatacaaggt atcgccaata ccctgccttt atgaaaacct ctgtctcgtg
     4981 gttagcggcc aattactcgc cggaagcagg aacaataaag atgaattcaa tttcacgaga
     5041 ccggatgaaa acccaacacg aacgctataa taccgaatga tgaacggatg aatgaatgat
     5101 acacagatgt taacatggcg gcccaataga cgcaaggcgc gattgggcca agcgaagggc
     5161 aggcactttg cctctgaccg ctatccgaat aattcatcgt tcatctcctg ccgcccataa
     5221 ggtcgctcgg gcgcagatga cccggcccgc ccacgcatct cgtcatagaa ccgtttgtca
     5281 tagcgtcgcg agcggacggg gataggcatc ttgacccccc agcccagcag caacacttcc
     5341 tctttctcct gcaagcgagc cagcatgccg cgcaactggt cgcgcccggc caggccgctg
     5401 agcacggcgc ggatgtcgtc ctcgtcgccc agccagccgg tgacgcgcgt gcccagttgc
     5461 gacatcacct cgtcgtcgat gcccgacggc cgctgatcga cgatgagcag cgtcacattg
     5521 tacttgcgca gctcacgggc gatagttccg aaggccgtct gacgtgagac gccggggctg
     5581 agcagcttgt gggcctcctc gacaacgatg accagcggcc gcggcgcgcc gacattgcta
     5641 agcttggcgt gttccgtctt gtctacccat gccttacgga tgcgccgggt caaaatattg
     5701 gagacgatga tgtaatcaag ttcattgtcg tgaacgccaa agctcagaat gatgtggcgg
     5761 ccgtgctcca gctgatcgga tatggctttc acagcatcaa cagccggttt gttaaccaca
     5821 tacggcttat gaaagatagg ctccaatttg cgatgcaatg attcggccgc tgtgggatga
     5881 atattatgac cattagccca gtgggctacg gaatcgggag cggggatttt tttgccgtta
     5941 tcatcctcaa tgaccgcgcc gggcttcatc ttcataaatt cgctgaacca cctaccttcg
     6001 aagactcgct ccagagcgga taatgtgacg gcagcagtgt cattcaagtc gagtgcttcg
     6061 gcgattaaca ggatgtcatc ggcattgaaa tcgccgagag caagctcaag ggtgaaatca
     6121 ggcgaattgt cacctacgcg cgtccccttg cccagagcag caatctgcac cttctcgccg
     6181 aacatcgcct tcaggccgcg tacctgttcg ccacgatcac tatcgcgatc gtcgaaacca
     6241 tactcgttgt gcatatcgaa caccagcgcc gcggccacgt cggtcttcat cagcccggcc
     6301 agcaccatgc gcgtcaggaa actcttgccc gtgcccgtcg cgccgaagat gccgctgctg
     6361 cgcttgacga agcgctccag atcgaggcag acgggatagc cctgctcgat ggtgtggccg
     6421 atgacgaagt tgacgccacc ttcctcgccg aagatggcgg ccacgtcgcc ggcatcggcc
     6481 gggcgcaccg gcgcgtggtg ggccggcaca gtcttcaccg gcttcgggcc ggggttctcc
     6541 tcgcccatgt ccacccgcgc ctgccactcg gcccgctccg gagaggtcaa ctccggcccc
     6601 aggtcgagca gcagcgtggg gtacatctcc agcgtcgtat agagcgtctt gccgtagagc
     6661 gcgccctgaa tcgccgcggg cagccggtcg gatttctcgt cggcgaagcg cggatcggtg
     6721 gagccgagtt gcagatcggt gatcagcccg tagtagcgat agcgcggcgt ctcgcagacg
     6781 acgaagccgc cttcctgcac ggtgtcggcc gcgacggtca gacgcacacg cagcccgctc
     6841 ttcagcgcgc cgccgacgat atagcccaac ggggcgttgg ggcccaatgg gccggatgat
     6901 ccctcagatt tttttggctg gtcgaacatc aaaagccccc catcatgtcg ggcacgccgc
     6961 ccaggccggg cagcgcctcg cccaatgcct cgagcaccgg caccagcgtg tcgtaatcgc
     7021 tgcgcaacaa ctcgatgacc t
//
LOCUS       DAOMQG010000157         7032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4601_length_7032_cov_9.545363, whole genome shotgun sequence.
ACCESSION   DAOMQG010000157 DAOMQG010000000
VERSION     DAOMQG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_7032_cov_9.545363"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLC06_09650"
     CDS             <1..765
                     /locus_tag="PLC06_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90750.1"
                     /translation="ALHAMEHNGAVLLGAFEDDRLIGFVFGVLGTEDNPNRMDQVAAA
                     RLKMYSVIAGVLPEYQKHDIGYRLKMAQRDFALRIGIRLITWTYDPLESLNARFNIGK
                     LGAICHHYLPNFHGDMTGINTGLPTDRFEAEWWVTQDRVAARAERKWRPLRLESMLAD
                     GALLVNEATINEAGMPVPPLDYLSRPSKQMLVEIPANFQNIKRADMALAQRWRMHTRD
                     IFRNMFDSGLVVTDFVFHEDERGNPRSYYLLTYQDS"
     gene            770..1891
                     /gene="menC"
                     /locus_tag="PLC06_09655"
     CDS             770..1891
                     /gene="menC"
                     /locus_tag="PLC06_09655"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRO90751.1"
                     /translation="MKIEQIDLYHVSMRLVSPFVTSFGPQQQRDCLLMAVHSEGLIGW
                     GESVATNDPGYSYETTGTAWHILSDFLIPALVGKGIAGPEALAPALSFVRGHPLAKSG
                     LDQAFWDLAAQRDGISMAAKLAQPYAEGAKARVPVGVSIGIQSSLERTMEVIGEYYDQ
                     GYRRIKLKIKPGYDVALGRAARAAFPDLPIMLDANSAFRLEDAAVFQAMDDLNLLMLE
                     QPLGYEDIYDHSQLRPLIKTPLCLDESIHSDDHARYALAIGACDIINVKPSRVAGWTE
                     ARRIHDRCRAAGMPLWVGGMLETGIGRAAQLALASLPGFTLPGDISATERYYERDITA
                     PFYLNREDSTITVPSGVGLGVEIDYDYLDAVTLRRESFN"
     gene            2008..2847
                     /gene="proC"
                     /locus_tag="PLC06_09660"
     CDS             2008..2847
                     /gene="proC"
                     /locus_tag="PLC06_09660"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRO90752.1"
                     /translation="MFSDKNVAFIGSGIMGEAIIRGLLTQNIVKPEQIYAADPLQARL
                     DDLRERYGIHVTGDNSEAAEAGQVIVLSTKPQNLAEVMPGIRGHLRRQDLLLSILAGT
                     PIRKLADGVAHASVVRAMPNTPAQIGQGMTVWTSTPEVTDEQRGQAQVILAALGQELY
                     VNDESYLDMATALSGTGPAYIFMFMESLIDAGVHLGFPRRVAEQLVFQTMRGSLEYAA
                     QSNKHVAELRNQVTSPGGTTAAALYQMEKGGLRTVISRAIWAAYQRSVELGQGEKVKG
                     PNQ"
     gene            2858..4612
                     /gene="pabB"
                     /locus_tag="PLC06_09665"
     CDS             2858..4612
                     /gene="pabB"
                     /locus_tag="PLC06_09665"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HRO90753.1"
                     /translation="MRSEIINEPVVVLQVDGEWRRFVNPVRVVVAETAADVAAALREI
                     DRSVEGEGLYAAGYLAYEAGAAFGLATYTPDLDAPPLLWFGLFRDYEPVDVPAADTTY
                     RFGEWRPSLSFDVYEAAIGRIKDAIAAGDTYQINYTFPLRATFEGDPWALFVSLAAAQ
                     RADYCAYVDMGRFAVCSASPELFFRLDGLTLTSRPMKGTAPRGLTSADDRRQIDWLHN
                     SGKNRAENVMIVDMIRNDFGRIARTGSVDVPALFTIERYPTLLQMTSTVTAETASPLS
                     EILAATFPCASITGAPKVSTMRIINDLEPNPRGVYTGSIGFIAPGRRAQFNVAIRTAT
                     IDRQRGRAVYHVGSGVVWDSDARDEYDECLLKARVLHVRPDNVTDFRLLESLRWTPDE
                     GYFLLEHHLERLINSAEYFNFRLDRAAVEGALTEAAAALPGPSKIRLLADRAGQIESE
                     ALPLEKGALPVPLGVGLALQPVSPADVYLYHKTTRREAYDAARASCPDCDDVILWNER
                     GELTESTVANLVLEIDGRRYTPPASSGLLAGTMRACLLERGELTERVLLPADLSRASR
                     IWLINSVRGWMPAIIRSS"
     gene            4710..5777
                     /locus_tag="PLC06_09670"
     CDS             4710..5777
                     /locus_tag="PLC06_09670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRO90754.1"
                     /translation="MNKFRVVIVLFLAGLGLFWVNRSASAAGGCYILSNDVGYVCDAN
                     PPASSFVQPPLRPDSILPGRKYGRLADNINVYSEPSMSSPIVRNAGDGYLWHSIEDTA
                     NNQGEGRLWYLIEPGEWVRAEDIKLGTTSQFTGFEILTNPERPFGWMLVDYWYSDAPG
                     EEPSPTALKLPRYTFVEVYDAEIDDEGWIWYDIGYGRWMKQTYLSIIEFNPKPKEIGK
                     DEFWVEVDLYEQNFVAYEGDRMVYAGLVSSGLNRWPTYEGLFQVWERHMETNMVGAEG
                     KVDYYVNEDVPHTMYFDFDIALHGAYWHDRFGYKHSHGCVNMPPRDAEWIYFWSENAP
                     NDLWVWVHTSDPMNYFEANQG"
     gene            5933..6712
                     /locus_tag="PLC06_09675"
     CDS             5933..6712
                     /locus_tag="PLC06_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO90755.1"
                     /translation="MGANFDLTGKVALITGGSRGIGLAIAEAYVAAGAKVVIVGRRQE
                     SLDEAVKSLQHNGGDAIGIAAHTGDGVAVTALVERVVEHFGGIDILVNNAATNPHFGP
                     FLTAEDGHWDKILDVNVKGYFRVAKACIPHMRERGGGKIINMASVAGLEPQPMMGVYC
                     VSKAAVLMMTEVLAAEVAADNIQVNAIAPGFVKTKFSQVLWSTPAIHDKLVNAVPQKR
                     MAEPSELAGIAVYLASAASSFTTGATFVVDGGQLAKGLNVG"
     gene            complement(6719..>7032)
                     /locus_tag="PLC06_09680"
     CDS             complement(6719..>7032)
                     /locus_tag="PLC06_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRO90756.1"
                     /translation="VRRNMPEILASQRKMLIRRGEDPDKMDDAQMALLFEKHLKQIDE
                     WLKGQPNFRVLYVHYSDVLADPRPQIAKINEFLGDNLNTQAMIQAIDPKLYRNRQADK
                     T"
BASE COUNT         1342 a         2043 c         2159 g         1488 t
ORIGIN      
        1 gcgctccatg cgatggaaca taatggcgcg gtgttgctgg gcgcgttcga ggatgaccgg
       61 ctgattggct ttgtctttgg cgtactggga accgaggata accccaatcg catggatcag
      121 gtggctgccg cccggctaaa gatgtattcc gtgatcgccg gagtgctgcc tgagtatcag
      181 aaacacgaca tcggttatcg gctcaagatg gctcaacgcg acttcgcctt gcgcatcggc
      241 attcggctca ttacctggac ctatgacccg ctggaaagcc tcaacgcgcg tttcaacatc
      301 ggcaaacttg gcgcgatttg tcatcactat ctgcccaact tccacgggga tatgacgggt
      361 atcaacaccg gcctgcccac cgatcgattt gaggcggagt ggtgggtgac ccaggaccgt
      421 gtggccgcca gagccgaacg caaatggcgg ccgttgaggc tggagtcgat gctggccgat
      481 ggcgcgctat tggtcaacga ggcgactatc aacgaagccg gtatgcccgt cccgccgctg
      541 gattacctca gccgcccgag caagcagatg ctggtcgaaa ttccggctaa ttttcagaat
      601 atcaagcgcg ccgatatggc tctggcgcag cgctggcgaa tgcacacccg cgatattttc
      661 cggaatatgt ttgattccgg ccttgttgtt accgattttg ttttccacga ggacgagcgc
      721 ggcaatccgc gcagctacta tcttctgact taccaggact cctgatacca tgaaaattga
      781 acagattgac ctttatcatg tcagcatgcg gcttgtcagc cccttcgtca ccagcttcgg
      841 gccacagcag cagcgcgatt gcctgctgat ggccgttcat tccgagggcc taattggctg
      901 gggcgagagc gtggccacca acgaccccgg ctactcctac gagacgaccg gcaccgcctg
      961 gcatatcctg agcgatttcc tcattcccgc gcttgtcggc aagggcatcg ccggaccgga
     1021 ggcgcttgct ccggcgctat ccttcgtgcg cggccatccg ctggccaaat cgggactgga
     1081 tcaagccttc tgggatttgg ccgcccagcg cgacggcatc tccatggccg ccaagctggc
     1141 ccagccatat gccgaggggg cgaaggcgcg ggtgccggtg ggagtcagca tcggcattca
     1201 gtcgtcgctg gagcgcacca tggaggtcat cggcgaatac tacgaccagg gctaccggcg
     1261 catcaagctc aagatcaagc cgggttatga cgtggcgttg gggcgggcag cgcgagcggc
     1321 tttccccgat ctgccgatca tgctcgacgc caactcggcc tttcggctgg aggatgcggc
     1381 cgtctttcag gcgatggatg atttgaacct gctcatgctg gagcagccgc tcggctatga
     1441 ggacatctat gaccatagcc aactgcggcc gctcatcaaa acgccgttgt gcctcgacga
     1501 gtccatccat tccgacgatc acgctcgcta tgccctggct atcggtgcgt gcgatattat
     1561 caacgtcaag ccgtcgcgcg tggccggctg gaccgaggcg cgacgcatcc atgatcgctg
     1621 ccgggcggcg ggcatgccgc tgtgggtcgg cggtatgctg gagacaggta tcggccgtgc
     1681 ggcgcaactg gccctggctt cgctgcccgg tttcacgctg cccggtgaca tttcggccac
     1741 tgagcgttac tacgagcgcg acatcaccgc gccgttctat ctcaaccgcg aggacagcac
     1801 gatcaccgtc ccgtccggcg tgggactggg agtcgagatt gattacgatt atctcgatgc
     1861 cgtcacgctg cgccgcgaat cgtttaacta acgcttgcgc ggcactttgc ctgatatcac
     1921 gacaggttta tagccgacca ctttagagcc gaggggcgtg acgaaagtgc cggctacagg
     1981 gtaaagacat ttattatgga tcaaacgatg ttttccgata aaaacgtggc tttcatcggc
     2041 agcggtatca tgggcgaggc gatcattcgc ggcttgctga cgcagaatat cgtcaagcct
     2101 gagcagatct acgccgccga tcctttgcaa gcgcgactgg atgacctgcg tgagcgctac
     2161 ggcatccacg tcacgggcga taactccgaa gcggccgagg cggggcaggt gatcgttctc
     2221 tccaccaagc cgcaaaacct ggctgaggtg atgcccggca ttcgcggcca tctgcggcgg
     2281 caggacttgc tcctgtcgat cctggccggg acgccaattc gcaagttggc cgacggcgtg
     2341 gcccacgctt cggttgtgcg cgccatgccc aataccccgg cccaaattgg ccaggggatg
     2401 accgtctgga catccacgcc cgaagtgaca gacgagcagc gcggacaggc gcaggtcatc
     2461 ctcgccgcat tgggtcagga actttatgtc aatgatgagt cctacctcga catggccacg
     2521 gcactgagtg gcaccggccc ggcctatatc ttcatgttta tggagtcgct cattgatgcc
     2581 ggtgtccatc tgggctttcc gcgccgcgtg gccgagcaac tggtcttcca gacgatgcgc
     2641 ggttcgctgg aatacgccgc ccaatcgaac aagcacgtcg ccgaactgcg caatcaggtg
     2701 acgtcgcccg gcggcacgac ggccgcggcg ctctatcaga tggagaaggg tggcctgcgc
     2761 acggtcattt cgcgcgccat ttgggccgcc tatcagcgct cggttgagct gggtcagggc
     2821 gaaaaggtga aaggccctaa ccagtgataa gtggcgcgtg agaagcgaaa taattaacga
     2881 gccggtcgtt gttctgcaag tagacggcga gtggcgacga ttcgtcaacc cggttcgggt
     2941 cgttgtggcc gaaacggcgg ccgatgttgc cgcagccttg cgggagatcg atcggagcgt
     3001 tgagggcgag gggctatacg ccgcgggata tcttgcctat gaggccgggg cagcctttgg
     3061 tttggccact tacacgcctg acctggatgc accgccgctg ctctggttcg gcctttttcg
     3121 cgattatgaa cccgttgacg tgccggcggc cgacaccacc taccgctttg gcgaatggcg
     3181 accctcgctg tcgtttgatg tctacgaggc ggccattggt cgtatcaagg acgccattgc
     3241 cgcgggcgac acctatcaga tcaactacac atttcctctc cgcgccacat tcgagggtga
     3301 tccctgggcc ttgttcgtca gcctggcggc ggcgcagcgg gccgattatt gcgcctatgt
     3361 ggacatgggc cgctttgcgg tttgttccgc ttcgccggag ttgtttttcc ggctggacgg
     3421 tttgaccctg acctcgcgac ccatgaaggg caccgcgccg cgtggtctga cttcggccga
     3481 cgaccgccgg caaatcgact ggctgcataa ctccgggaag aaccgcgccg aaaatgtgat
     3541 gatcgtggat atgatccgca acgatttcgg ccgcattgcc cgcaccggca gcgtcgatgt
     3601 accggcgttg ttcaccatcg agcgctaccc gaccttgctg caaatgactt cgaccgtcac
     3661 ggccgagacg gcctcacccc tgagcgagat tctggccgcg acctttccct gtgcctctat
     3721 caccggcgcg ccgaaagtca gcacgatgcg catcattaac gatctggagc ctaatccgcg
     3781 cggcgtttat accggatcca ttggcttcat cgcaccgggc cgccgagcgc agttcaacgt
     3841 tgccattcgc acggcgacta tcgaccggca gcgcggtcgg gccgtctacc acgtcggcag
     3901 cggcgtggtg tgggattccg acgcccggga tgagtacgat gaatgtttgt tgaaggcgcg
     3961 cgtgctccat gtccggccgg acaacgtgac cgacttccgg ttgctcgaat ctctccgctg
     4021 gacgcctgac gagggatact tcttgctgga acaccacctg gaacggctga tcaactcggc
     4081 cgagtacttc aacttcaggc tggacagggc ggccgttgag ggtgccctga ccgaagcggc
     4141 cgctgccctg cccggcccaa gcaagatacg gctgctcgcc gaccgggctg ggcaaatcga
     4201 gagcgaagcg ctccctctgg aaaaaggcgc gctgcctgtg ccgcttggcg tgggtttggc
     4261 cttgcagccg gtgtcgccgg ccgacgtcta tctctatcac aaaacgaccc gccgcgaagc
     4321 gtatgatgct gcacgtgcct cgtgtcccga ttgcgacgat gtgattctct ggaacgaacg
     4381 tggcgaatta accgaaagta ctgttgccaa cctggtgctg gagatcgacg gccgtcgcta
     4441 tacgccgccc gcctcttcgg ggttattggc cggcacaatg cgggcttgcc tgctggagcg
     4501 gggcgagttg acggaacgcg ttctgttgcc ggcagacctc tcgcgagcga gccgcatttg
     4561 gctgatcaat tcggttcgcg gctggatgcc cgcgatcata cgctcctcat aatacggcca
     4621 acgccttgaa tcgaatgcat ggcccgtgct agaatgggtg caatatcaac cggatttcat
     4681 aacccgggta ttgtaatgat gtttcgtcta tgaataaatt ccgcgtcgtc atcgtcttgt
     4741 tcctggccgg attgggcctg ttttgggtta atcgctctgc ttcggcggcg ggcggttgct
     4801 atattctctc caatgatgtt ggctatgttt gcgacgccaa tcctccagcc tcatcctttg
     4861 tccagccgcc gctgcggccc gacagcatat tgcccggccg caaatatggc cggttggccg
     4921 acaatatcaa tgtctattcc gaaccgagca tgagcagtcc catcgtccgt aatgccggcg
     4981 acggttatct gtggcacagc attgaagaca cggccaacaa tcagggagag gggcggctat
     5041 ggtacctgat cgagcctggg gagtgggttc gcgccgagga catcaagctt ggcaccacgt
     5101 cccaattcac cggttttgag attctgacca accccgagcg gccgtttggc tggatgctgg
     5161 tcgattactg gtacagcgac gcgccgggcg aggagccatc tccgacggcc ctcaagttgc
     5221 cgcgctatac tttcgtcgag gtctatgatg ccgagatcga cgacgaaggc tggatctggt
     5281 acgacatcgg ctatggccgg tggatgaagc agacctatct gagcatcatc gagtttaacc
     5341 cgaaaccgaa agagatcggc aaagatgagt tttgggtcga ggtcgacttg tacgagcaaa
     5401 actttgtcgc ctatgagggc gaccgcatgg tctatgccgg acttgtctcc agcggtctaa
     5461 atcgctggcc gacctatgag gggctgttcc aggtctggga gcgccatatg gaaacaaata
     5521 tggttggcgc ggaaggcaag gtcgactact acgtcaacga agacgtgccc catacgatgt
     5581 acttcgactt cgatatcgcc cttcatggcg cgtactggca cgaccgattc ggttacaagc
     5641 acagtcacgg ctgcgtcaac atgccgccgc gcgacgccga gtggatctac ttctggtcgg
     5701 agaacgcgcc caatgatctg tgggtgtggg tccatacctc tgacccgatg aactatttcg
     5761 aggcgaatca ggggtagatc agtaatcagt gatcagtaga tcagttggcg ccggctttgt
     5821 ccggcgcttt ttatttcatg ggattgctca aacgaaaagg caggtgtata agagtgttga
     5881 tattttgtcg atgggtagcc gtcggcggtc attgcgggag gtatggataa cgatgggagc
     5941 gaacttcgat ctgacgggca aggtggccct cattaccggc ggctcgcgcg gcattggcct
     6001 ggcgattgcc gaagcctacg tcgcggccgg cgcgaaggtg gtcatcgtcg ggcgcaggca
     6061 ggagtccctc gatgaagccg taaagagcct gcagcacaac ggtggcgacg caataggcat
     6121 tgccgcccac accggcgacg gcgtggccgt tacggcattg gtagagcgtg tagtcgagca
     6181 ttttggcggc atcgacattc tggtcaacaa cgctgccacc aatccccatt ttggcccatt
     6241 tctgacggcc gaagatggcc attgggacaa gatactcgac gtcaacgtga agggctactt
     6301 ccgcgtcgcc aaggcctgta ttccccacat gcgcgagcgc ggcgggggaa agatcatcaa
     6361 tatggcctct gtggccggcc tggagccgca accgatgatg ggcgtatact gtgtcagcaa
     6421 ggcggccgtc ctgatgatga ccgaagtgct ggcggccgag gtggcggccg acaatattca
     6481 ggtgaatgcc attgcccccg gctttgtgaa gacgaagttt agccaggtgc tgtggagcac
     6541 gcccgctatc cacgacaagc tggtcaatgc cgtgccgcag aagcgcatgg ccgagccttc
     6601 ggagctggcg gggattgccg tctatctggc ctcggccgca tccagtttca ccaccggcgc
     6661 aacgttcgtg gtcgatggcg ggcagttggc caaggggctg aatgtgggct aggcaacgtt
     6721 aagttttatc ggcttgccga ttccgataca gcttcgggtc tatcgcctgg atcattgcct
     6781 gggtattaag attgtctccc aggaactcgt tgatcttggc gatttgcggc cgcgggtcag
     6841 ccagcacatc gctgtagtgg acgtaaagca cgcgaaagtt gggttgaccc ttgagccatt
     6901 cgtcgatctg ttttagatgc ttctcgaaca gcagcgccat ctgcgcgtcg tccatcttgt
     6961 cggggtcttc gccgcgacgg atgagcatct tgcgctgcga ggccaggatt tccggcatat
     7021 tgcgacgcac aa
//
LOCUS       DAOMQG010000158         7022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4608_length_7022_cov_8.618631, whole genome shotgun sequence.
ACCESSION   DAOMQG010000158 DAOMQG010000000
VERSION     DAOMQG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4608_length_7022_cov_8.618631"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PLC06_09685"
     CDS             <1..1152
                     /locus_tag="PLC06_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90757.1"
                     /translation="SLLFTLTGPDGKEYFREARPILPDDDTRWDDAADNRLSYDLPLP
                     PGLPPGVYALALTPEGGPAIPLGPVEIAPTTTWPVAPELLFDEVPAVDTKYEIRDTNS
                     EAPSSLVSRISYLVSYPAVIPWDDAVLPGNNLPLTLYWRVAPGGADLTDLRYRLEVIA
                     PDGAVLRSQESKPGASWLGALPGGALLREDTSLYFRPDVAPGRYRLRWTLLDGDTPLG
                     EPATSGHVVVESWPLETNVPAAPYVIDRGQGAGFGPAIRLHSYYSGVPTNGLLNIAFY
                     WQATARPDTDYVVFVHLVDEAGNIVSQIDSVPVGGARPTTTWRAGEVITDIHNLLVPD
                     DLPPGTYHLNVGLYNPDDGTRPAVVVGGAPQPDNQLRLAPTFDLPWGTP"
     gene            1149..3500
                     /locus_tag="PLC06_09690"
     CDS             1149..3500
                     /locus_tag="PLC06_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRO90758.1"
                     /translation="MNRRRVLLGLILAAWLAITLAYGALNPLFEAPDEHWHFFTAAHI
                     ADTGRLPRVTNPPDEWLGQEAAQPPLYYLLGGLLIVPFDTGGARDAVRLNPFAWIGDA
                     SALTNINRMIHTPAEARPWADWATAAHLLRVFSTLLGLGTLLCIYGSGRRLWPESTTR
                     PLMATALVAFLPQFNFLHASITNDALVIFLCSLALYQLIDLTGGRAQPVGPSGTSSRP
                     PVRSSSFRLVLLGLTIGLAALSKNAGALLLLYSLGFLGVVALRDGQLRRYWRTALLVA
                     GPALLVAGWLWLRNWQLYGDWTATAPFIAIAGGDRGYTLGQVLGESKGLLLSLVAVFG
                     WFNLRPPEWVYWLWAAIAGLAAVGALACIARRNTRYEIRDTSEEDGSKLDTAHPPLST
                     AHPPLITGHWPLITDHWPLTTALLLALWPLLVYAGLAAFMLRTEAAQGRLLLPAVLPL
                     ALGVAWGLSGWGRCAPGALGKFNRRLAPLVALAALATTLYCLLFVIRPAYQRPPAIAV
                     IPPTARLVLPELIERGRGVSLLAAEVETEQAAPGDVVRLTLYWTAATPPAASPEFVLE
                     LFGRDMQRIANLHSYHGRGLYPADLWEPGAIIADRFAVRIAPDAEAPVLGRVFVRIND
                     GAPGIEVATVKIVPREPLPAPDGWAAELGDRIALVGAAIRPEQALPGQTIEVLVRWYV
                     PVGVPDRDYTALIHLGQPDAEPLATGDSPPLGGAYPTSAWGNGETIDDVYFLTLPTDL
                     LPGRYPVWIGLYDPATGERLPVKVEGELQPNGVYLAGWVEVGE"
     gene            3528..4154
                     /locus_tag="PLC06_09695"
     CDS             3528..4154
                     /locus_tag="PLC06_09695"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRO90759.1"
                     /translation="MQFDLFDARAPHFATLDDLVAAMMKLEDDPLFPAGTNMVIFRGN
                     PKARLMIIGEAPGTEENRQGKPFVGRSGQLLDQILGSVHLDPEQDVFITNAVFRLPPG
                     DDGKPLRKPTSDEIAWYKPWLLEIIRLVDPLIMLLTGNVATEAILGQTGITKLRGQWF
                     HVDGRWAMPIFHPAYLLRNPSREPNAPKALMWRDIKEVRRKYDELMAA"
     gene            4406..4810
                     /locus_tag="PLC06_09700"
     CDS             4406..4810
                     /locus_tag="PLC06_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90760.1"
                     /translation="MTRHIASEKARAHWREILDAVAAGEEIVIERYGRPVATLAPYPR
                     DLAPGVREPSPIYDSTDLHALKEEIAVDVAAHILQIIGPPLTWPEGLALLQKQVADAG
                     GLGFGDDPDAIVEQMRKTRQEIFEADYAHLYR"
     gene            4791..5216
                     /locus_tag="PLC06_09705"
     CDS             4791..5216
                     /locus_tag="PLC06_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO90761.1"
                     /translation="MLICIDSCVFIRAADAPLSDAARLIRLLSPGLPVVIPRLVVREV
                     LRNLRTDDAIHTFYRLFSGKSGGEIIDLPVPDDLVAAYVARGLRAKGDAYIGAFAEWM
                     RVDCLISDNRHFLRDLQTDAYRLLSPGEFLELLTTEPKP"
     gene            5213..5941
                     /locus_tag="PLC06_09710"
     CDS             5213..5941
                     /locus_tag="PLC06_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO90762.1"
                     /translation="MTHSDKAAERGEPGYVWRSGQDRRLAMIREWSDLSGRILDNGTG
                     LGTWLEACGRANPDNVRIGLEIEFDRAVEALVRADGIALGIGEHLPFADDSFDLILSN
                     EVLEHVADDRACAAEMARVTRPGGRIVVFAPNRWYPVEQHGVYWRGEYYFGNIPLVNY
                     LPDPLRNRLAPHVRTYMARGLRRLFDGLPVRVLHHGRVFGGYDNIARRRPRLGGAIRR
                     ALYTAEGTPLSVLGLSHFLVLEKM"
     gene            5989..6234
                     /locus_tag="PLC06_09715"
     CDS             5989..6234
                     /locus_tag="PLC06_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90763.1"
                     /translation="MKTAISLPDALFEQAEQVAERLKLNRSQLYARALQAYLDRYDPD
                     SITAAFNAVYSIESPELDPALWEMQYLTLKENNEDEW"
     gene            6228..6584
                     /locus_tag="PLC06_09720"
     CDS             6228..6584
                     /locus_tag="PLC06_09720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRO90764.1"
                     /translation="MVDDRLIKQGDVFWADLPAPRGSGPGYRRPVVVIQQTAINASDI
                     NTILCVPLTTQTRLSDSPGNVLLSIKQTGLSKVSVANVSQLVSLDKRFLDAYVATLPR
                     HTLEDILDGVEFMMGR"
     gene            6589..>7022
                     /locus_tag="PLC06_09725"
     CDS             6589..>7022
                     /locus_tag="PLC06_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90765.1"
                     /translation="MDRKFYLEDIPLDDAWAAFRAALDEASLWRPLPAETVPLSEANG
                     RVTAAPVWARLSSPHYHAAAMDGYAVRAGDTVGALDASPVLFVVEEGKGGKGAGEKGR
                     KGEREKGRSPLHPCTPSPLLPFSPAPPAQPVNTGHPLPAWADA"
BASE COUNT         1128 a         2558 c         2148 g         1188 t
ORIGIN      
        1 tcgctgctgt tcacgctgac cgggccggac ggcaaggagt atttccgcga ggcgcgcccc
       61 atcctgcccg acgacgacac gcgctgggac gacgcggccg acaaccgcct cagctacgac
      121 ctgccgctgc cgcccggcct gccgcccggc gtctatgcgc tggccctgac gcccgaaggc
      181 ggccccgcga tccccctcgg cccggtcgag atcgccccga cgaccacctg gcccgtcgcg
      241 ccggaactgt tgtttgatga agtgcctgcg gtagatacga aatacgagat acgagatacg
      301 aattcggaag caccctcttc actcgtatct cgtatctcgt atctcgtatc ttatccagcg
      361 gtcatcccct gggacgacgc cgtgctgccc ggcaacaacc tgcccctgac cctctactgg
      421 cgcgtggctc cgggcggggc cgacctgacc gacctgcgct accggctgga agtgattgcc
      481 cccgacggcg cggtgctgcg cagccaggag agcaagcccg gcgcttcgtg gctcggcgcg
      541 cttcccggcg gggcgctgct gcgcgaggac accagcctct acttccggcc cgacgtcgcg
      601 cccggccgct accggttgcg ctggacgttg ctcgatggcg atacgccgtt gggcgagccg
      661 gccacaagcg gccacgtcgt cgtcgagtca tggccactgg agacgaatgt gcccgccgcc
      721 ccctacgtca tcgaccgggg gcagggagcc ggcttcggcc ccgccatccg gctgcacagc
      781 tattacagcg gcgtgcccac caacggcctg ttgaacatcg cgttctattg gcaggcgacg
      841 gcccggccgg acaccgacta cgtggtgttc gtccatctgg tcgacgaggc cggcaacatc
      901 gtcagtcaga tcgattccgt gcccgtgggc ggcgcgcggc cgaccaccac ctggcgcgcg
      961 ggcgaggtca tcaccgacat ccacaacctg ctcgtccccg acgacctgcc gccgggcacc
     1021 tatcacctca acgtcggcct ctataacccc gacgacggca cgcgcccggc cgtggtcgtg
     1081 ggcggcgcgc cgcagcccga caaccagtta cggctggccc ccacgttcga cctgccctgg
     1141 gggacgccat gaaccgccgc cgcgtcctgc tcggcctgat tctggccgcc tggctcgcca
     1201 tcaccctggc ctatggagcg ctcaacccgc tcttcgaggc ccccgacgag cattggcact
     1261 tcttcaccgc cgcccacatc gccgacaccg gccgcctgcc gcgcgtcacc aacccgcccg
     1321 acgagtggct ggggcaggaa gcggcccagc cgccgctcta ctatctgctc ggcggcctgc
     1381 tcatcgtccc gttcgacacc ggcggcgcgc gcgacgccgt ccggctcaac ccgttcgcct
     1441 ggatcggcga cgcctcggcc ctgaccaata tcaaccgcat gatccacacc ccggccgagg
     1501 cccggccctg ggcagactgg gcgacggccg cccatctgtt gcgcgtcttc tccacgctgc
     1561 tggggctggg cacgctgtta tgtatctacg gcagcggccg ccggctctgg cccgaatcca
     1621 cgacgcggcc gctcatggcc accgccctcg tcgccttcct gccccaattc aactttctcc
     1681 acgcctcgat caccaacgac gcgctggtca tcttcctgtg cagcctggcg ctctaccagc
     1741 tcatagacct gacaggtggg cgcgcccagc ccgttggccc gtcagggacc tcatcgcgcc
     1801 cacctgtcag gtcttcttct ttccgcctcg tcctgctcgg cctcaccatc ggcctggccg
     1861 ccctcagcaa gaacgccggc gcgctgctgc tgctctacag cctcggcttc ctgggcgtgg
     1921 tcgccctgcg cgacgggcaa ctgcggcgct attggcgcac ggcgctgctg gtggccgggc
     1981 cggcgctgct ggtggccggt tggctgtggc tgcgcaactg gcagctctac ggcgactgga
     2041 cggccaccgc gccgttcatc gccatcgccg gcggcgaccg cggctacacc ctcggccagg
     2101 tgctgggcga gtccaagggg ctgctcctgt cgctggtggc cgtcttcggc tggttcaacc
     2161 tgcggccgcc ggagtgggtc tactggctct gggcggccat cgccgggctg gcggccgtgg
     2221 gcgcgctggc ctgcatcgcg cggcgaaata cgagatacga gatacgagat acgagtgaag
     2281 aagacggctc caaactggac acagcccatc cgccgctgtc cactgctcat ccgccactga
     2341 tcactggcca ctggccactg atcactgatc actggccact gaccactgcc ctcctcctcg
     2401 ccctctggcc gctcctcgtc tatgccggtc tcgccgcctt catgctgcgc accgaggcgg
     2461 cccaggggcg gctgctgctg cccgccgtgc tgcctctggc gctgggggtg gcctgggggc
     2521 tgtccgggtg gggccgctgt gctcccggcg cgctgggcaa gttcaaccgg cggctggccc
     2581 cgctggtggc gctggcggcg ctggcgacga cgctctattg cctactgttc gtcatccgcc
     2641 ccgcctacca gcggccgccc gccatcgccg tcattccccc cacggctcgc ctcgtgctgc
     2701 cggagctgat cgagcgcggc cggggtgtgt cgctgctggc cgccgaagtg gagacggagc
     2761 aggccgcccc cggcgacgtg gtccgcctga cgctctactg gacggccgcc acgccgcccg
     2821 ccgcgtcgcc ggagttcgtg ctggaactgt tcggccgcga catgcaacgc atcgccaatc
     2881 tccacagcta ccacggccgc ggcctctacc cggccgacct gtgggagccg ggcgccatca
     2941 tcgccgaccg cttcgccgtg cgcatcgccc ccgacgccga agcgccggtg ctgggccgcg
     3001 tcttcgtccg catcaacgac ggcgcgccgg gcatcgaagt cgccacggtc aagatcgtgc
     3061 cgcgcgagcc gctgcccgcc cccgacggct gggcggccga actgggcgac cgcatcgccc
     3121 tcgtcggcgc cgccatccgc cccgagcagg cgctccccgg ccagaccatc gaggtgctgg
     3181 tgcgctggta cgtgcccgtc ggcgtccccg accgggacta caccgcgctc atccatctgg
     3241 gccagcccga cgcggagccg ctggccaccg gcgacagccc gcccctcggc ggcgcctacc
     3301 ccaccagcgc ctggggcaac ggcgagacta tcgacgatgt ctacttcctg accctgccga
     3361 cggacttgct tcccggccgc tatccggtct ggattggcct ctatgacccc gcgacgggcg
     3421 agcgcctgcc ggtgaaagtg gagggagagc tacagcccaa cggcgtctac ctggcgggct
     3481 gggtggaggt cggagaatga taacgacaaa cagacaggag gatacccatg caattcgacc
     3541 tctttgacgc gcgcgccccc cacttcgcca ccctcgacga tctcgtggcg gccatgatga
     3601 agctggagga cgacccgctc ttccccgccg gcacgaatat ggtcatcttt cgcggcaacc
     3661 ccaaggcccg gctgatgatc atcggcgagg cccccggcac cgaggaaaac cgccagggca
     3721 agccgtttgt cggccgctcc ggccagttgc tcgaccagat tctcggctcc gtccacctcg
     3781 acccggagca ggacgtgttc atcaccaacg ccgtctttcg cctgccgccg ggcgacgacg
     3841 gcaagccgct gcgcaagccc acctcggacg agatcgcctg gtacaagccc tggctgctgg
     3901 agatcatccg cctggtcgat ccgctcatca tgctgctgac cggcaacgtg gccaccgaag
     3961 ccatcctggg gcagacgggc atcaccaaac tgcgcggcca atggttccac gtcgatgggc
     4021 gctgggccat gcccatcttc caccccgcct acctgctgcg caatccctcg cgcgagccga
     4081 acgctcccaa ggcgctcatg tggcgggaca tcaaggaagt gcgccgcaaa tacgacgagt
     4141 tgatggccgc atgacgatag acctgacagg tggggcgaac gaagccatcc cgcggcaacg
     4201 tggcgccgcg ccccacctgt caggtctggg tgtgatcctg agcgtgacag cttacaacca
     4261 ataaggtaca attatcctgt acgaagctta cagaacgtac ggtgacgata gacctgacag
     4321 gtggggcgaa cgaagccgtc ccacggcaac gtggcgccgc gccgccccac ctgtcaggtc
     4381 tgggtgtacc ggaatgaggc aaaccatgac ccgacacatt gccagcgaga aagcgcgcgc
     4441 ccattggcgc gaaatactgg acgccgtcgc cgccggcgag gagatcgtca tcgagcgcta
     4501 tgggcggccg gtggcgactc tggcccccta cccgcgagac ttggcgcccg gcgttcgcga
     4561 gccctcaccc atctatgact cgaccgatct ccacgcgtta aaagaagaga tagccgtcga
     4621 tgtagccgct cacatcctcc agatcatcgg cccccctctc acctggcccg aagggctggc
     4681 cctgctgcaa aagcaggtag ccgacgcggg tgggctgggt tttggcgacg accccgacgc
     4741 catcgtggaa cagatgcgca agactcgcca ggaaatattc gaagccgact atgctcatct
     4801 gtatcgatag ctgcgtcttc attcgcgccg ctgatgcccc gttatccgat gccgcgcgtc
     4861 tgatacggtt gctcagcccc ggtctaccgg ttgtcattcc ccggttggtc gtccgcgaag
     4921 tgctgcgtaa cctgcgcact gacgatgcca tccacacctt ctaccggcta ttctccggca
     4981 aatcaggcgg cgagattatc gacctgccgg tgcctgatga tctggtagcc gcctacgttg
     5041 cgcgcggact acgcgccaaa ggcgacgcct atatcggcgc gttcgccgaa tggatgcgcg
     5101 tcgactgcct gatctccgac aatcgccact tcctgcgcga tctccaaacg gatgcctatc
     5161 ggctcctttc gcccggtgaa ttcctggagt tgttgacgac ggaacccaaa ccatgaccca
     5221 ctccgacaag gccgccgagc gcggcgaacc cggctatgtc tggcgctccg gccaggaccg
     5281 gcggctggcc atgattcgcg agtggtcgga cctgagcggc cgcatcctcg ataacggcac
     5341 cggcctcggc acctggctgg aagcctgcgg ccgcgccaac cccgacaatg tgcgcatcgg
     5401 cctggagatc gagttcgatc gggccgtcga ggcgctggtg cgggccgacg gcatcgccct
     5461 cggcatcggc gagcatctgc ccttcgccga cgacagcttc gacctcatcc tgagcaacga
     5521 ggtgctggaa cacgtggccg acgaccgggc ctgcgcggcc gagatggccc gcgtgacgcg
     5581 ccccggcggc cgcatcgtcg tcttcgcccc caatcgctgg tatccggtgg agcagcacgg
     5641 cgtctattgg cgcggcgaat actatttcgg caacatcccg ctggtcaact acctgcccga
     5701 tcccctgcgc aaccgcctcg ccccccacgt gcggacgtac atggcgcggg gcctgcgccg
     5761 cctgttcgac ggcctgccgg tgcgtgtgct ccatcacggc cgcgtcttcg gcggctacga
     5821 caacatcgct cgccgccgcc cgcgactggg tggggccatt cggcgcgcgc tctacacggc
     5881 cgaggggacg cctctctctg tgctggggct gtcgcatttt ttggtgctgg agaaaatgta
     5941 gctccctcat gcattgacac aggtataaca aaacgttata ctctcctcat gaaaaccgcc
     6001 atatccttgc ccgacgcgct ctttgaacag gccgaacagg tggccgagcg actcaagctc
     6061 aaccgcagtc agctctatgc ccgcgcgctg caagcgtacc tggatcgcta cgaccccgac
     6121 agcatcaccg ccgcgttcaa tgccgtctac tcaatcgaat ccccggaact cgatccggct
     6181 ctgtgggaga tgcaatatct cacgctcaaa gagaacaatg aggatgaatg gtagacgatc
     6241 ggttgatcaa acagggggat gtattctggg ctgatttgcc cgcgccgcgc ggttccgggc
     6301 cgggctatag acggccggtg gtcgtcatcc agcagaccgc cattaacgcc agcgatatta
     6361 atacgatctt gtgtgtgcca ctgacgactc aaactcgcct gagtgattcg ccgggcaatg
     6421 tattgctctc gattaagcaa accgggcttt ccaaagtctc ggtcgccaat gtctcgcaac
     6481 ttgtttccct cgataaacgt tttcttgacg cctacgtcgc taccctgcct cgccatacat
     6541 tggaagatat attggacggc gtcgaattta tgatgggacg atagctcaat ggaccgcaaa
     6601 ttctacctcg aggacatccc tctcgatgac gcctgggccg ccttccgcgc cgcgctggac
     6661 gaggcgagcc tgtggcggcc gctgcccgcc gagaccgtgc ccctcagcga ggccaacggc
     6721 cgcgtcacgg ccgcccccgt ctgggcgcgc ctctcctccc cccactacca cgccgccgcc
     6781 atggacggct acgccgtgcg cgccggggac accgtggggg cgctggatgc gtcgccggtg
     6841 ctgtttgtgg tggaggaggg gaagggagga aagggagcag gggagaaagg gagaaagggg
     6901 gaaagggaga aggggagatc acccctgcac ccctgcaccc cttcacccct tctccccttc
     6961 tcccctgctc ccccggctca gcccgtcaac accggccacc cgcttcctgc ctgggccgac
     7021 gc
//
LOCUS       DAOMQG010000159         6996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4629_length_6996_cov_10.161648, whole genome shotgun sequence.
ACCESSION   DAOMQG010000159 DAOMQG010000000
VERSION     DAOMQG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_6996_cov_10.161648"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1191
                     /locus_tag="PLC06_09730"
     CDS             118..1191
                     /locus_tag="PLC06_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRO90766.1"
                     /translation="MTSKLTQVRQSFDKWNVDGLLVTSPANRRWLSGFTGSAAMLLIT
                     REQAILSTDSRYWEQAEHEAPDFVVYRARAKSDDFSNFLAMGGGAKRLGFEARHVSVS
                     DYRNYRKLKDYRWKALNMTVEPFRAIKTEEELALIRKAAFITDETVSRFPEMARPGKT
                     EREVAWDLEKTMRELGADKPGFDIIVASGPNSALPHHHPGDRRLERGDMIVVDLGSEV
                     NGYRSDLTRTFHLGDAPDDSFWSIYNTVADAQLAAINGIRPGANGKYVDSLARDLIVA
                     AGHGEHFGHGTGHSLGLEIHEFPRFSIHSEKDVLQAGTVMTVEPGIYLPGFGGVRIED
                     LVLVTPDGRELLSHAPKSPVIPI"
     gene            complement(1171..2400)
                     /locus_tag="PLC06_09735"
     CDS             complement(1171..2400)
                     /locus_tag="PLC06_09735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO90767.1"
                     /translation="MRRILLLTPQLPYPPHQGTSLRNFHMLKALAQVADITLLSMTES
                     VNSTDLSPLEPYCRVLPPVLVPDRSQGTRIRQLISSSLPDMGLRLRSDAFASALNKAL
                     RSDDYAAVQIEGIELASYIPQIRSASPSARIVLDCHNAEAELQRRARAVDRHNPSRWL
                     AAIYSTIQTGRLARFESWALTASDAILAVSEIDRAQLLKLLPTGAIDITIVPNTIDVV
                     EYGQGEPPSEDINHDLVFTGKMDYRPNVDGVLWFAEKVWPALRDARPDITWAIVGQKP
                     HARLDGLRGKEGITITGFVPHIQPYLAGALIYIVPLRIGSGTRLKILEAMTAGLPIVS
                     TRIGAEGFDVSDQQEMVLADSPEEWVENILRLLNDQERRTEMGAAAHEFAKRYDWRTI
                     IPLMKEVYSNLLDGNHG"
     gene            complement(2400..3479)
                     /locus_tag="PLC06_09740"
     CDS             complement(2400..3479)
                     /locus_tag="PLC06_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO90768.1"
                     /translation="MGGISQYTIHLIAALGELETGDEYTIFHNRRETRDFRPPGRHSF
                     LRSNLWTPCHHRYERYTLGVEIARHHLDVLHSPDFIPPSFGASRRVITVHDLNFIHFP
                     EFLTPESHRYYTNQIEWAVSVADHIIADSEATRLDLIDLLGASPAAVTTIHLAANPIY
                     SAAYSAAEIRATTQALGTGEGFVLGVGTLEPRKNWPLLIRAYADLRRQYDVAVPLVLV
                     GGRGWIYEEIFQTIARLDLGPHVMHLSGVTDIQLAHLYRAAGVLAFPSYTEGFGLPAL
                     EAMHAGCPVIVSNRGSLPEVVGDTALKLDPTDLEGWVEALAKVLMDRDYADTLARAGL
                     EQAKEFTWRKTAMATSAIYHEVSAK"
     gene            3635..4900
                     /locus_tag="PLC06_09745"
     CDS             3635..4900
                     /locus_tag="PLC06_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90769.1"
                     /translation="MTKNQNARPTSGAYDQEIGELRKRLEWLDEERRKSSRKLAEIEQ
                     RAELQNRELANRERRIQELEKQLSLVNSQLSRIPMVDTQLSQFRDDIVKMIDQYDKRR
                     IEAEKELDRLRRVEHEATTREIADIRKELPAIGRIEQELPLRQAEEARLANLIGVQQN
                     TITQLSTQVENVDRSLTFLSEKEKQDSRNIGEIQATLLEISKRWEPITNRLEILAQNV
                     TRVQTTAQTTGEAQVLLRKSISDWTEQVQIGEHERNQRLAGWQRAMDEHEASFQRFNQ
                     DWVKFNNQYNDSRTAVETMKSLQTHLEQQQREANELVRVESKRMESRWEQFTQEDARK
                     WKNFEADSLQRLSINDRRERELREQIADLEGELGRLSTELDTIVRVQTAQSEAIKKWP
                     LAWLEEVEKALDQNPHRRRQPALVPVRED"
     gene            4912..6789
                     /gene="selB"
                     /locus_tag="PLC06_09750"
     CDS             4912..6789
                     /gene="selB"
                     /locus_tag="PLC06_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRO90770.1"
                     /translation="MHVIGTAGHVDHGKSTLVEALTGIDPDRLAEEKSREMTIDLGFA
                     WLTLDDQEVGIVDVPGHRDFIENMLAGVGGIDLALFVIAADEGVMPQTREHLAILDLL
                     KINRGVVVLTKSDLIDDPDWLDLVILEVAVTLQGTTLSEAPIIPVSARTGAGIADLKT
                     AISHLLAEIALPDDWGKARLPIDRVFSLPGFGTIVTGTLLDGSLRVGDEVSIQPGNLT
                     GRIRGLQTHKTKRNVAHPGNRVAINLTGIAREDIARGQVVALPGLLRETKLIDAQYRH
                     LDGAAAPLTHNAEVKLFVGSAEVTARVRIIGQEKIESGTDGWVQLALVAPIAIMHDDR
                     FILRRPSPGETIGGGRILDPNPGRRHRRFREDVIRRFQARAQGSADDLLLNTLARLGP
                     VSHKNLIDQTGLPAAEAENAIRTLTADERIIDLGKVLMVTPAWNAQRDRLLNTLANYH
                     QATPLRAGMEREAVRSKLQTPQVVFNAMVGELVAANLLAEAGTLLKLPDHEVVLSEKD
                     EAAVVQLMNLLERQGILSPSVKECKAVVGDGVYYALVDLGRLKPVGEDVVYSAEMYDQ
                     LIKQLTNYLHLKGSISAPEARDLLGASRKYAIALLEHMDELRITRRSGDIRLPARPIK
                     E"
BASE COUNT         1724 a         1866 c         1931 g         1475 t
ORIGIN      
        1 ctcgctttag cgcctgaaaa ggtgaataaa tttctggaca tcattcccga gtcggccgtg
       61 ttgggttcgg tcgaggaagg agatggccac atctggatag ttaactgaga aataaaaatg
      121 acttcgaaat taactcaagt tcggcaatca tttgataaat ggaatgtgga tggtttgctg
      181 gtcaccagcc cggctaatcg acgctggctg agcgggttta ccggctcggc cgcgatgctg
      241 ctcatcactc gagagcaagc gattttgtct acagattcgc gttattggga acaggcagag
      301 catgaagcgc cggattttgt agtataccgg gcacgtgcaa agagtgatga cttctcaaat
      361 ttccttgcca tgggtggtgg tgccaagcga ctgggttttg aggcaagaca tgtttccgtg
      421 agtgattaca ggaattatcg taaacttaag gattatcgct ggaaagcgct gaatatgacg
      481 gtcgagcctt tccgagcgat caaaacggag gaggagttag ccctgatccg caaggctgcc
      541 ttcatcaccg atgagacggt ctcccggttc ccagagatgg ccaggccagg gaaaacagaa
      601 cgagaggtcg cctgggatct ggaaaagaca atgcgcgaat tgggagccga taagccggga
      661 tttgacatca tcgtcgcctc cggccccaat agcgccctgc cgcatcatca cccgggggat
      721 cgccgcctcg aacgcgggga tatgatcgtc gtcgatcttg gttcagaagt gaacgggtat
      781 cgaagcgact tgacccgcac cttccatcta ggtgatgccc cggatgactc tttctggagc
      841 atttacaata cggttgcaga tgctcagctg gcagcaatta acgggattcg tcccggtgca
      901 aatggcaaat atgttgattc cctggcccgc gatctcatcg tcgccgccgg tcatggagaa
      961 catttcggcc acggcacggg ccatagtctg ggcctggaaa tccatgagtt tccgcggttc
     1021 agcatccatt cggagaaaga tgttcttcag gcaggcacag tgatgacggt cgaaccgggc
     1081 atctatcttc ccggattcgg cggcgtcagg atcgaggacc tggtgcttgt cacacccgat
     1141 ggacgggaat tattgagcca cgcgccgaaa tcacccgtga ttcccatcta gcagattaga
     1201 gtagacctcc ttcataagtg ggataatcgt tcgccaatca taacgcttgg cgaattcgtg
     1261 cgcggccgct cccatttcgg ttcgtcgttc ttgatcattc agcaggcgaa ggatgttctc
     1321 cacccactcc tccggggaat cggccaatac catttcctgt tggtcactta cgtcgaatcc
     1381 ctctgcccca atacgggttg acacgatagg taaaccagcg gtcatggctt ccaggatttt
     1441 taatcgtgtc ccacttccta tgcgaagggg aacaatataa atcaaagccc cggcaagata
     1501 aggttggata tgcgggacga atccagtaat cgtgatgcct tcttttccac gcaggccgtc
     1561 tagtcgggca tgaggctttt ggccgacgat tgcccaagtg atatcaggac gtgcatctcg
     1621 cagcgcgggc cacacttttt ccgcaaacca gagaacgccg tccacgttcg gccgatagtc
     1681 catcttgcct gtaaaaacca ggtcatggtt gatatcttcg gagggaggct caccttggcc
     1741 atattcgaca acatcaatcg tgttgggcac aatcgtgata tcgatcgcgc cggtcggcaa
     1801 taacttaagc agctgtgcgc gatcgatttc gctcacagcc agaattgcat cggatgcggt
     1861 caacgcccag gattcgaaac gagccagcct tcctgtttgg atggtcgagt agattgccgc
     1921 gagccagcga gacgggttgt gccggtctac cgcccgcgcc cgacgttgca gctctgcctc
     1981 cgcgttgtgg caatccaaca cgatcctggc cgaaggactg gcgcttctga tttgcgggat
     2041 ataggaggcc aactcaatgc cctcgatctg aacagccgca tagtcgtccg agcgtaatgc
     2101 tttattcaaa gcggaggcaa atgcgtcact cctcaggcga agccccatgt ccggcagaga
     2161 tgagctgatg agttgcctaa ttcgtgtgcc ctgtgaacgg tcgggaacga gaacgggtgg
     2221 aagcacgcgg cagtacggct ccagaggcga cagatccgtt gaattcacac tctcagtcat
     2281 gcttaagagc gtgatatcag ccacctgagc cagggctttc agcatatgga aattacgcag
     2341 gctggtgcct tggtgcgggg gatagggtag ttggggagtc aatagcagga tacgtctcat
     2401 tattttgccg acacttcatg ataaatcgct gatgtagcca tggctgtttt ccgccaagtg
     2461 aattccttcg cctgttcaag cccggcgcga gccagcgtgt cggcatagtc gcgatccatc
     2521 agcactttcg ccaaggcttc cacccacccc tcaagatcag tcggatccag tttgagagcc
     2581 gtatcaccca caacttcggg aagcgagcct cgattactga cgataaccgg gcaaccggcg
     2641 tgcatggctt ccagcgccgg taatccaaat ccttcggtgt atgatggaaa agccagcact
     2701 ccagcggctc gataaagatg ggccaactgg atgtctgtca ccccggagag atgcatgaca
     2761 tggggtccca gatcgaggcg agcgatagtc tggaagatct cctcatatat ccatcctcga
     2821 ccgcccacca aaacaagcgg cactgcaacg tcatattgac gacgaagatc ggcataggca
     2881 cggattagca ggggccagtt ctttcgaggt tccagcgttc cgaccccgag gacaaagcct
     2941 tctcccgtcc ccaacgcctg agtagtggcg cgtatctccg cggccgagta agcggccgaa
     3001 tatatcgggt ttgccgcaag atgaatcgtc gttacggccg ccggagatgc ccctaacaga
     3061 tcaatcaaat ccaggcgggt cgcctcactg tcggcaataa tatggtcggc aaccgaaacg
     3121 gcccactcga tttgattggt atagtagcgg tgactctcag gcgtcaggaa ttcggggaaa
     3181 tgaatgaagt tcaagtcatg gacagtgatg accctgcgac ttgcgccaaa cgacggcggg
     3241 atgaagtcgg gagaatgaag gacatccaga tgatggcgag ctatctccac cccaagggtg
     3301 tatctctcgt agcgatgatg acagggtgtc cataaattac tacgcaaaaa gctgtgcctt
     3361 cccggagggc ggaagtctcg tgtctcacgc cgattatgaa atatagtgta ctcatcgccc
     3421 gtctccaatt cccccaatgc cgcgatcaga tggatcgtgt actggctaat gccacccatc
     3481 tgatatgtcg gcagacgaca atcgataccg atatgcatgg aacgattgta aggcgcatta
     3541 gtgaatcaaa gcaagttggg cagtgtgtaa gccgatcagt ataatgcgta aattcaaccg
     3601 tgcttcaaca ggcatggcgc tgaggtaaat cgcgatgaca aagaaccaaa atgcccgacc
     3661 gaccagcggc gcatatgacc aggaaatcgg agagttgcgc aaacgactgg aatggctgga
     3721 tgaagaaaga cgaaaatcaa gccgtaagct cgccgagatt gagcaacgcg ccgaactcca
     3781 gaaccgagag ctagcaaatc gcgaaagacg catccaggaa ctggagaagc agctttccct
     3841 cgtcaattcg caactatcgc gcatcccaat ggtcgacaca cagctctccc agttccgcga
     3901 cgacatcgtt aagatgatcg accaatatga caaaaggcgc atcgaagcgg agaaggagct
     3961 ggacaggctg cgtcgggtcg aacatgaggc cactacacgc gagatagcgg atattcgcaa
     4021 ggagctgccg gccatcggcc gtatcgaaca ggaactcccc ctgaggcaag ctgaagaagc
     4081 ccgtctggcg aacctgattg gcgtccagca aaataccatc acccagctct ccactcaggt
     4141 ggaaaacgtg gacagatcac tcaccttcct ttccgagaag gagaagcagg acagccgcaa
     4201 catcggcgag atacaggcca ccttgcttga aatcagcaag cgatgggagc ctatcacgaa
     4261 tcgattggag atcctggccc agaatgtgac aagggtccaa acaacagctc agacaacggg
     4321 agaagcacag gtcctgctgc gcaaaagcat tagtgattgg accgaacaag tgcaaatcgg
     4381 tgagcacgaa cggaatcagc gtctggccgg ctggcaaagg gcaatggacg aacacgaggc
     4441 ttcgttccaa cgctttaatc aggactgggt gaaatttaat aatcagtaca acgactctcg
     4501 cactgccgta gaaacaatga agtctttgca gactcatctg gagcagcagc agcgagaagc
     4561 aaacgagctt gtgcgcgttg aatccaagcg catggagtcc cgatgggaac aatttactca
     4621 ggaagacgca cgcaagtgga agaatttcga ggctgattca ttgcaacggt tgtcgatcaa
     4681 tgatcgtcgt gaaagagaac tacgcgagca aattgcggac ctggagggag agttaggacg
     4741 gctttcaacc gaattggata cgattgtccg cgttcagaca gctcaatccg aggctatcaa
     4801 gaagtggccg ctcgcgtggc tggaggaagt agaaaaggct ctggatcaga acccccatcg
     4861 ccgtcgtcaa ccggctctcg taccggtgcg agaagattaa acaatacatc catgcacgtc
     4921 atcggtacag ccggtcacgt cgaccacggt aaatctacgc ttgtagaagc cctgacgggg
     4981 atcgatccgg atcggctcgc ggaagaaaag tcccgagaga tgaccatcga cctgggcttt
     5041 gcctggttga cgctcgatga ccaggaggtc ggcatcgtcg atgttcccgg tcatcgcgat
     5101 tttatcgaga atatgctggc tggagttggg gggatcgatc tggccttgtt tgtgatcgcg
     5161 gccgacgaag gcgtgatgcc ccaaacgcgc gaacacttgg ccatcctcga tttattgaag
     5221 atcaatcgcg gcgtcgtggt cttgacaaaa agcgatttga tcgacgatcc ggactggttg
     5281 gatctcgtca tccttgaagt cgccgttacg ctgcaaggaa caacgttgtc agaggcccca
     5341 atcatcccgg tatctgcacg aaccggcgcg gggattgccg acctgaaaac agccatcagt
     5401 catttattag ccgaaatcgc cctgccggac gactggggaa aagcgcgatt accgatcgat
     5461 cgcgtttttt cgttaccggg cttcggcacc attgtcaccg gcaccctcct cgatggcagc
     5521 ctgcgcgtgg gtgacgaggt ttccattcaa cccggaaacc tgaccggccg tatacgcggc
     5581 ttgcagacac ataaaaccaa acgaaatgtc gctcatcccg gcaaccgcgt ggccataaat
     5641 ctgacaggga tcgcccgaga agatatagcg cgtggccagg ttgtcgcctt gcccggcttg
     5701 ctgcgcgaaa caaagctgat cgatgctcaa taccggcatc ttgatggcgc ggccgcccca
     5761 ctcacacaca acgctgaagt caaactattt gttggctcgg ctgaagtcac cgcgcgcgtc
     5821 cgaatcatcg gccaggagaa aatcgaatcg ggaaccgatg gctgggttca gttggcatta
     5881 gttgccccta tcgctatcat gcacgacgat cgattcatcc tgcgacgccc ttccccagga
     5941 gaaacgattg gtggcggccg cattctcgat cccaatcccg gccggcgtca tcgtcgcttt
     6001 cgggaagatg taattcgtcg cttccaggct cgagcccaag gctctgccga cgatttgctc
     6061 ctcaacacac tcgctcggct tgggcctgtg tcgcacaaaa accttatcga tcagaccggc
     6121 cttcccgcgg cagaggccga gaacgccatt cgaacgctta ccgccgatga acgcataatc
     6181 gatctcggca aggttttaat ggtaacccct gcctggaatg cccaacggga tcgattgctg
     6241 aatacgctgg caaactatca tcaggctacc ccactgcgtg ccgggatgga gcgcgaggca
     6301 gttcgtagta aactacagac cccacaagtg gtcttcaatg cgatggtcgg cgagctggta
     6361 gccgcgaacc tgctggccga agccggaacg ctgctgaagc tgcccgacca tgaagtcgtg
     6421 ctgtcggaaa aagatgaggc ggccgtcgtc caactaatga atttgctgga aaggcaaggc
     6481 atcctctcgc cgagtgtgaa ggaatgcaag gccgtggtag gcgacggcgt gtactatgca
     6541 cttgtcgacc tgggacgact taagccggtt ggcgaagatg tcgtctactc ggccgagatg
     6601 tatgaccagc taatcaaaca gctaacgaac taccttcatt taaagggatc aatctccgcc
     6661 ccagaggccc gcgacctgct tggcgccagt cgaaaatatg cgatcgccct cctggagcac
     6721 atggatgagc tgcgtatcac gcgtcgttcc ggtgatattc gtttgcccgc ccgacccata
     6781 aaagaataga accgccgaga agggattatt gacattcctc acttgacata ttgttcagag
     6841 aacactatga ttattatgta atgtgtttcg gcacggaagt tcgtgctggt ttgtccattt
     6901 ttctgaacac cacacccaat atatagggta gcacgatcaa caggagcgtc atcaatggat
     6961 aggtggcatg catttggaag agcacgtttt gtcctt
//
LOCUS       DAOMQG010000160         6960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4652_length_6960_cov_10.634178, whole genome shotgun sequence.
ACCESSION   DAOMQG010000160 DAOMQG010000000
VERSION     DAOMQG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4652_length_6960_cov_10.634178"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLC06_09755"
     CDS             <1..1609
                     /locus_tag="PLC06_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90771.1"
                     /translation="WTTRSLVLLALTIFLTAATQALFGLARNSSFEFMAQRVERDSRD
                     ELYASLLGKSQTFHNHQRIGDIMARATNDVRTLNVMFSPGLMLITDSLVGLMAPIVLI
                     YRINPQLLLVPALFVLLFIITIRGYARQLAPVSEGLRDSFGVMNAGLAETVNGIEVVK
                     GSAQEQREGEKFIDNASVYRDYAVREGEIQARYLPMLVFSVCWGLAFLHAMLLWRGGA
                     LTLGQVVSYVGLFGFLRFPTFVSVFSFQLAQLGVASARRILELINAETDLDENESGVS
                     RPISGKVEFRGVSFGYGAGEQGNRGEREKGGEESLPPILKGLTFTINPGETVAIVGQT
                     GSGKSTLTRLINRIFDAAGGQVLVDGIDVRDWNLESLRSQISTIEQDVFLFSRTIAEN
                     IAFGVPNATQADIEEAAREAQAHDFIMSFDKGYETEAGERGVALSGGQRQRIAIARAF
                     LTNPRILILDDSTSAIDSATEDRIQQAMYRISQQRTTFLITHRLSQIRWADRILVLKA
                     GEIVDQGTHDELLEKSPDYQRIFARYE"
     gene            1626..2420
                     /locus_tag="PLC06_09760"
     CDS             1626..2420
                     /locus_tag="PLC06_09760"
                     /inference="COORDINATES: protein motif:HMM:NF022022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HRO90772.1"
                     /translation="MATGALIPIEQKTILFYEDELTAVRAGDGRIFVPVRPIVERLGL
                     NWSGQYSRIRRDPVLSQVQGVCVIQTPGGRQEAVSIPLDYLSGFLFGINADRVKPEYR
                     EDVIRYQMECYKVLSEALTEGRLITDITFDDLLQTADPGAVQAYQIAQAVVRLARNQI
                     LLESRLSGRIDDHEGRLITLESQLGDTGRNVTPDQASQLSQAVKAVAIELGKKSGRNE
                     FGAIYGELYRKFGITSYKMLPARRFDEAMKFLTDWHETITGAAGPF"
     gene            2550..4475
                     /locus_tag="PLC06_09765"
     CDS             2550..4475
                     /locus_tag="PLC06_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90773.1"
                     /translation="MGFILDGLETESYDRQYSDRELLDRIIGYFRPHAKAMTLVAVMI
                     TLNSVMGALGPIVIARSVGLLETNPATTTMILIALGVSLIGASAWGFNYIRQKFSARV
                     TSNVVLQLREDVFNATVRHDMSFYDEHPSGKIVSRVTSDTQDFAEVVNLTLNMLSQLL
                     LVVMLIAYLLTVNPTLTLLLIAMAPFAAAIALSFRRLARRVTQNARRVNATINAQIQE
                     SISGILIAKGFRQESAIYATFDANNRQGYSVGITRGLTLSGIFPLMGLASGLGTAIVF
                     YFGGLATRTSIAPAEWYLFMQTVGFFWFPVTSIASFWSQFQDGLSAAERVFALIDREP
                     RVQQTGSEPVGPRITNYELRITNLEEDTETDGSELVSRISNLVSPPPTRGHIEFRHVR
                     FSYTDREVVLPDFSLDIRAGETIALVGHTGAGKSSIGKLITRFYEFQGGEILLDGRDI
                     RSLDLGQYRMQIGLVPQEPFLFAGTVADNIRYGRPEATDDDVIAAAHAVSGGEWLASL
                     PRGLQTDVGERGGSLSMGQRQLVALARVVLKDPAIFVLDEATASIDPFTESQIQEGLQ
                     EIMRDRTSLVIAHRLFTVRHADRIIVMREGAIIEQGKHEELLAAGGHYAELYNTYFRH
                     QSLDAILDPTWRTGGDA"
     gene            complement(4504..6309)
                     /locus_tag="PLC06_09770"
     CDS             complement(4504..6309)
                     /locus_tag="PLC06_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO90774.1"
                     /translation="MEMYPDPDATEQDDEIDDIDNMDDAAPEERGPEPLDVDALVRKA
                     RRSRQIAESDVQSILASVSEEQAEQLYERLQKLNIRIVSADGELVDDPGDIGLLNRLD
                     DLDDEDEDSLHLSDAEDDPVHTYLKEIGQVPLLSAEQEIWLATQMQAQRVLDRLNQDV
                     GLSRSATGNGRRGRKGAPAEQTPSQSADRSANPDALRAKAMLLNYHNLLECWQCVLDS
                     SAHIKVAPPELIPLFDEARKLRVDWQRNERSYVHRYLNDGTWGQDEAWTDLAECLFSV
                     FTAFYLLPDGLADEVAGHIRLTGALPPAAEVDDWLFPPDSSLMYNEFMIVHLAEEAKG
                     NLTRANLRLVVSVAKRYMGRGIQLLDLVQEGNVGLLRAVEKFDHTKGFKFSTYATWWI
                     RQAVSRAIADQARTIRIPVHMYETINKIVKTQRELVQKLGHEPSTEELALAPELEYLT
                     PEESAAIKLAQETGRPIDPMLERKWRTAVSKIRDILRIAMDPMSLETPVGNNDDATEL
                     GDFLADESILEPGDAASRELLREHIRTVLEALNDREREVLEMRFGLKDGTDHTLEEVG
                     KTFGVTRERIRQIEAKALRKLRHPSRSRTLRDYLQ"
     gene            complement(6410..>6960)
                     /locus_tag="PLC06_09775"
     CDS             complement(6410..>6960)
                     /locus_tag="PLC06_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90775.1"
                     /translation="KIATRRLRNANPLEENFLRQCLEHPGALSTVNYLLSRNKQRDVS
                     ADDFTRIEDKALLAIVAQRATVPTVATIEELCDSLDSVLAQRVKTLFAKPSSLKIKLE
                     RLPDTLALSVLDWRLEKIREHNTILKQLLPSGGQTEPGDELTRSYLDQVQEMGRINRA
                     RDAMSATSRRRAEEGHGGRRSR"
BASE COUNT         1270 a         2349 c         2020 g         1321 t
ORIGIN      
        1 ctggacgacc cgctcgctgg tgctgctggc cctgaccatc ttcctgaccg ccgccaccca
       61 ggcgctcttt ggcctggccc gcaactcgtc cttcgagttc atggcccagc gcgtggagcg
      121 cgacagccgc gacgaactct atgcgagcct gctgggcaag agccagacct tccacaacca
      181 ccagcgcatc ggcgacatca tggcccgcgc caccaacgac gtgcgcacgc tcaacgtcat
      241 gttcagcccc ggcctgatgc tcatcaccga ctccctcgtc ggcctcatgg ccccaatcgt
      301 cctcatctac cggatcaacc cgcagctgct gctggttccg gccctcttcg tcctgctgtt
      361 catcatcacc atacgcggct acgcccgcca actggccccg gtcagcgaag ggctgcgcga
      421 ctcgtttggc gtgatgaacg ccgggctggc cgagacggtc aacggcatcg aggtcgtcaa
      481 gggcagcgcc caggagcagc gcgaagggga aaagttcatc gacaacgcgt ccgtctatcg
      541 cgactacgcc gtgcgcgagg gcgaaatcca ggcccgctac ctgcccatgc tcgtcttcag
      601 cgtctgctgg ggcctggcct ttctccatgc catgctgctg tggcgcggcg gcgcgctcac
      661 cctgggccag gtggtcagct atgtcggcct gttcggcttc ctgcgcttcc ccaccttcgt
      721 ctccgtcttc tctttccagc tggcccagct gggcgtcgcc agcgcgcgcc gcatcctgga
      781 actcatcaac gccgagacag acctcgacga aaacgagagc ggcgtctcgc ggccgatctc
      841 cggcaaggtc gaattccgcg gcgtcagctt cgggtatgga gcaggggaac aggggaacag
      901 gggagaaagg gagaaagggg gcgaagaatc tctcccaccc atcctgaagg gcctgacctt
      961 caccatcaat cccggcgaga cagtggccat cgtcgggcag accggcagcg gcaaaagcac
     1021 cctgacgcgg ctcatcaacc gcatcttcga cgccgccggc ggccaggtgc tggtcgacgg
     1081 catcgacgtg cgcgactgga atctggaatc gttgcgctcc cagatatcca ctatcgagca
     1141 ggacgtcttc ctgttctcgc gcaccatcgc cgagaacatc gccttcggcg tgcccaacgc
     1201 cacccaggcc gacatcgagg aagccgcgcg tgaggcccag gcccacgact tcatcatgag
     1261 cttcgacaag ggctacgaga ccgaggccgg cgagcgcggc gtggccctct ccggcggcca
     1321 gcgccagcgc atcgccatcg cccgcgcctt cctgaccaac ccgcgcatcc tgatcctcga
     1381 cgacagcacc agcgccatcg acagcgccac cgaagaccgc atccagcagg cgatgtaccg
     1441 catcagccaa cagcgcacga ccttcctcat cacccaccgc ctcagccaga tccgctgggc
     1501 cgaccgcatc ctcgtcctca aggcgggcga gatcgtcgat caggggacgc atgatgaatt
     1561 gctggaaaag tcgcccgatt atcagcggat tttcgcgcgg tacgaatgag tatgattgag
     1621 gagttatggc aaccggcgca ttaataccca tcgagcaaaa gacgatcctt ttctatgagg
     1681 atgagctaac ggccgtacgt gcgggagatg ggcgcatctt tgtcccggta cgccccattg
     1741 tggaacgact tggccttaac tggagcggtc aatactcccg tattcggcgt gacccggtac
     1801 tcagtcaggt tcaaggtgtt tgcgtaatac aaacacctgg aggtcgacaa gaagcagtat
     1861 ctatcccact tgattacttg agtgggttcc tttttggaat aaacgccgat cgggtaaagc
     1921 cggagtatcg cgaagatgtg atccggtatc aaatggaatg ctacaaggtg ctttcggaag
     1981 cccttaccga agggcgactt attaccgata tcaccttcga tgacctcctc cagacggccg
     2041 atccgggtgc ggtacaggct tatcagatcg ctcaggcggt ggtgaggctc gcgcgcaatc
     2101 aaatattgct cgaatcccgg ctgtcgggcc ggatcgacga tcatgaaggc cggctcatca
     2161 ccctcgaatc gcagttgggc gacaccggcc ggaacgtgac cccggatcag gccagccaat
     2221 tgagccaggc ggtcaaggcg gtggccatcg aacttggcaa gaaaagcggc cgtaacgaat
     2281 ttggcgccat ctatggcgag ctttatcgca agttcggcat cacgtcctac aagatgctcc
     2341 ctgcccgccg ctttgatgag gcaatgaaat tcctgaccga ttggcatgaa accataaccg
     2401 gcgccgccgg accgttttaa caaccgacct accgacctac tgacctactg atctactggt
     2461 ctactgacct actgatctac tgacctactg atctactgac ctactgatct actgacctac
     2521 tgacctaccg acctactgga tactgaatca tgggcttcat cctcgacggt cttgagaccg
     2581 aaagctacga ccgccaatac agcgaccgcg aactgctgga tcgcatcatc ggctatttcc
     2641 ggccgcacgc caaagccatg accctcgtgg ccgtcatgat cacccttaac tcggtcatgg
     2701 gtgccctcgg ccccatcgtc atcgcccgct ccgtcggcct gctggagacc aaccccgcca
     2761 ccacgaccat gatcctgatc gccctgggcg tcagtctcat cggcgcgtcg gcctggggct
     2821 tcaactacat ccgccagaag ttctccgccc gcgtcaccag caacgtcgtg ctgcagctgc
     2881 gcgaagacgt cttcaacgcc accgtccgcc acgacatgtc gttctacgac gaacacccgt
     2941 cgggcaagat cgtcagccgc gtcacatccg acacccagga cttcgccgag gtcgtcaatc
     3001 tgacgctcaa catgctgagc cagctgctgc tggtcgtcat gctgatcgcc tacctgctga
     3061 ccgtcaaccc gaccctgacc ctgctgctca tcgccatggc ccccttcgcc gcggccatcg
     3121 ccctcagctt ccgccgcctg gcccgccgcg tcacgcagaa cgcccgccgc gtgaacgcca
     3181 ccatcaacgc ccagattcag gaatcgatca gcggcatcct catcgccaag ggcttccgcc
     3241 aggaatcggc catctacgcc accttcgacg ccaacaaccg ccagggctac agcgtcggca
     3301 tcacccgcgg cctcaccctg tcgggcatct tcccgctcat gggcctcgcg tccggcctgg
     3361 gcacggccat cgtgttctac ttcggcggcc tggccacgcg cacctcgatc gcccccgccg
     3421 aatggtatct gttcatgcag accgtcggct tcttctggtt ccccgtcacc agcatcgcct
     3481 cattctggag ccagttccag gacggcctct cggccgccga gcgcgtcttc gccctcatcg
     3541 accgggagcc gcgggtgcag cagacgggga gtgaaccggt ggggccgaga attacgaatt
     3601 acgaattacg aattacgaat ttggaagaag ataccgaaac ggacggctct gaactcgtat
     3661 ctcgtatctc caatctcgta tctcctccgc ccacccgcgg ccatatcgaa ttccgccacg
     3721 tccgcttcag ctataccgac cgtgaggtgg tgctgcccga cttctcgctc gacatccgcg
     3781 ccggcgagac catcgccctt gtcggccaca ccggcgcggg caagagcagc atcggcaagc
     3841 tcatcacccg cttctatgag ttccagggcg gcgagatatt gctcgacggc cgcgacatcc
     3901 gctcgctcga cctgggccag tatcgcatgc agatcggcct cgtgccgcag gagccgttcc
     3961 tcttcgccgg aacggtggcc gacaacatcc gctacggccg ccccgaagcc accgacgacg
     4021 acgtgatcgc cgccgcccac gccgtcagcg gcggcgagtg gctggcgtcg ctgccgcggg
     4081 gcctgcaaac cgacgtgggc gagcgcggcg gcagcctgtc gatgggccag cggcaacttg
     4141 tggctctggc gcgcgtggtg ctgaaggacc cggccatctt cgtcctcgac gaagccacgg
     4201 ccagcatcga tcccttcacc gagtcgcaga ttcaggaagg gctgcaggag atcatgcgcg
     4261 accgcacctc gctggtcatc gcccaccgcc tgttcaccgt gcgccacgcc gaccgcatca
     4321 tcgtcatgcg cgaaggggcg atcatcgaac agggcaaaca cgaggagctg ctggccgccg
     4381 gggggcatta cgccgaactg tacaacacct acttccggca tcagtcgctc gacgccatcc
     4441 tcgacccgac ctggcggacg ggcggggatg cctgacgagc ggccctaagg ctgattcagc
     4501 gttctactgc aaataatccc gcaacgtccg gctgcggctg gggtggcgca gcttgcgcag
     4561 ggccttggcc tcgatctgcc ggatacgctc gcgcgtgacg ccgaaggtct tgccgacctc
     4621 ttccagcgtg tggtcggtgc cgtccttcag gccgaagcgc atctccagca cctcgcgctc
     4681 gcgatcattc agcgcttcca gcaccgtgcg gatatgctcg cgcagcagct cgcggctggc
     4741 ggcgtcgccc ggctccagga tgctctcgtc ggccaggaag tcgcccaatt ccgtggcatc
     4801 gtcgttgttg cccacggggg tttccagcga catcgggtcc atggcgatgc gcaggatgtc
     4861 gcgaatcttg ctgacggccg tgcgccactt gcgctccagc atcgggtcga tgggccggcc
     4921 ggtctcctgg gccagcttga tggccgccga ctcttccggc gtcagatatt ccagctccgg
     4981 ggccagggcc agctcttccg tgctcggctc gtggcccagc ttctgcacca gttcgcgctg
     5041 ggtcttgacg atcttgttga tcgtctcata catatgaacg gggatgcgga tggtgcgcgc
     5101 ctggtcggcg atggcccggc tgaccgcctg gcgaatccac cacgtggcat aggtgctgaa
     5161 cttgaagccc ttggtgtggt cgaacttctc caccgcgcgc agcaggccga cgttgccctc
     5221 ctgcaccagg tcgagcaact ggataccccg gcccatgtag cgcttggcga cgctgaccac
     5281 cagccgcaga ttggcccgcg ttagattgcc cttggcttcc tcggccagat gcacgatcat
     5341 gaactcgtta tacatcagcg atgaatcggg cgggaagagc caatcatcga cctcggccgc
     5401 gggcggcagc gcgccggtga gccggatatg gccggctacc tcgtcggcca acccgtcggg
     5461 caagaggtag aaggcggtga agacggagaa caggcattcg gccaggtcgg tccaggcctc
     5521 atcctgcccc catgtaccgt cattgagata gcgatggaca taggagcgct cgttgcgctg
     5581 ccagtccacg cgcagcttgc gcgcttcgtc gaacaggggt atcagctccg gcggggccac
     5641 cttgatgtgg gcgctgctgt ccagcacgca ttgccagcac tccagcaagt tgtggtagtt
     5701 gagcaacatc gccttggcgc gcagggcgtc ggggttggcg ctgcgatcgg cgctctggct
     5761 cggcgtttgc tcggcaggag cgcccttgcg gccgcggcgg ccgttgccgg tggcgctgcg
     5821 cgacaggccg acatcctgat tcagtcggtc gagcacgcgc tgggcctgca tttgcgtggc
     5881 cagccaaatc tcctgctcgg ccgacaacag cggcacctgc ccaatctcct tgaggtaggt
     5941 atggacgggg tcatcctcgg cgtcgctcag gtgcagcgag tcctcgtcct catcatccag
     6001 atcgtcgagc cggttgagca gcccgatatc gccgggatcg tcgaccaact cgccgtcggc
     6061 cgagacgatg cgaatattca acttttgcag gcgttcgtag agttgctcgg cttgctcttc
     6121 gctcaccgaa gccaggatag attgcacatc cgactcggcg atttgtcgcg agcggcgggc
     6181 tttgcgaacc aacgcatcaa cgtccaatgg ctccgggccg cgttcttccg gcgcggcgtc
     6241 gtccatattg tcgatgtcgt cgatttcgtc gtcttgctct gtcgcgtccg ggtcgggata
     6301 catctccata cagtaaccac ttcaaagcat gagaacattg ttacttaagg aatcactatt
     6361 ttcaaagtca ggcaggggat ggcgtgattg attttgcacc ccaccctcat cagcgcgacc
     6421 ggcggccgcc atgtccctct tcggcgcgcc ggcggctcgt ggccgacatc gcgtcacggg
     6481 cgcgattaat gcgccccatt tcctgcacct ggtcaagata ggaccgggtc agctcgtcgc
     6541 cgggttccgt ttgaccgcct gacgggagaa gctgcttgag aatggtgttg tgttctctga
     6601 tcttttccag tcgccaatcg agcacagaca acgccagcgt atcgggcaag cgctccagct
     6661 tgatcttcag cgatgacggc ttcgcgaaaa gcgtcttcac ccgttgcgcc agcaccgaat
     6721 ccagactatc gcacaattcc tcaatcgttg caaccgtcgg cacggtggcg cgttgggcca
     6781 caatcgccag cagcgccttg tcctcgatgc gggtgaagtc gtcggccgat acatccctct
     6841 gcttgttgcg gctcagcaaa tagttgaccg tcgacagcgc ccccggatgc tccaggcact
     6901 gtcgcaggaa attctcttcc aatgggttgg cgttgcgcaa gcgccgcgtg gcgatcttgc
//
LOCUS       DAOMQG010000161         6937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4670_length_6937_cov_6.204301, whole genome shotgun sequence.
ACCESSION   DAOMQG010000161 DAOMQG010000000
VERSION     DAOMQG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4670_length_6937_cov_6.204301"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLC06_09780"
     CDS             <1..377
                     /locus_tag="PLC06_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90776.1"
                     /translation="AAATARPGESINLRLYWTSDGNISQSYKVFTHLLGDVFNAGGGN
                     FLWGQVDNEPAANTRPTTTWRAGEVIVDEYAIPIAPDAPSGTYRIEIGLYDPINGQRL
                     PALGPDGAPTADHLVLITVDIE"
     gene            472..1713
                     /locus_tag="PLC06_09785"
     CDS             472..1713
                     /locus_tag="PLC06_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90777.1"
                     /translation="MLRRLIPFLLAAVLVACDRSPAATPAATLPVAPAESAAGQAVPA
                     KTAGLTAEPGLPPTAAVFVPLLPSGGTQPTATPPPTVMPTEAPTDEAPVAPTATPYVS
                     NIPDFTPRPELGPSKMGIHVARPNSEAIMEFVRAAHPAVVKGVDDLGFLAEVKAVSPR
                     TVTVGRFSVDPQDYDGEPEAAAAALVARQLPQMLLNPAVDYWEGWNEPDPNLDRMDWY
                     ARFEAERVRQLAQHGLRAAVGGFSAGVPELNEFELFVPAIEEAQRHGGILTLHEYAAP
                     DITFLYGDPLPGYPTYPDRGPLMFRYRWFYRDILEPRGLVVPLVISEAGIDGILGNRP
                     GPDGLGWQDFQAFWVEQGWGPDGQTAFLNQLQWVDNEARQDPYVIGYALFTAGGARAW
                     TAYELAPFLPRLAEYIVSQKQ"
     gene            1798..3849
                     /locus_tag="PLC06_09790"
     CDS             1798..3849
                     /locus_tag="PLC06_09790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90778.1"
                     /translation="MRRSGGPAAIASPKLHQLSVSTSTPEPSTRRFMLIAAAIMLLAL
                     ALRLRGLDTTALWGDQAFTLNAAMRWVNGGDMPLAANKSSVGFVNPPMIEYLYAAALW
                     LWRDILSVSWLTLLGGLAAVAVTGFVTARLFGRRAGLWAMLAFAVAPWAVYWSQLIWN
                     QTMVPPFAALALGGLLLYLADRPRWHYLVISFVAAAIMTQVHPGSAVQLVTIALALLL
                     FRWRVRWRHVLVGALAFAALYLPYLVYQIGTGWADLGAMGDIAGQESAFSGAAALLSL
                     DLIRAQGLLRAVPGVVAFDNLATALFLAALGLVVWRLWRNAGRRLPDSPHSTPDSLPQ
                     PSERIALAVLLLWFFMPLLFYLRSSVYLQNYYLLSQWPAHFMILGIGLDTARRAAAGM
                     AAQATRPAKRQAWAVVGLILPALFLLSAAYQVFFSLRYQDARAAGHDSLMQVRHARAM
                     IAMSRRLLTEHEGWRLVGLGHGHQADNSDLALLQEFVDPRRVLLADSELALPLPRPYA
                     FLLNTRPGSLAAYTLNDLAYPVPDANIQVGDATWQFYEWTEPEQGTSSPPAASWALGL
                     DLAGYFPGEFVPGQETIVVLTWDINGPPPDALYHFGVYLLNERDEVVAQHDGPGFDSA
                     QWRAGDRFITYHRLALPPDLPPGTYRVAAALYSWPDLVRAELRDGGNTAYLQTITISA
                     P"
     gene            3985..4773
                     /locus_tag="PLC06_09795"
     CDS             3985..4773
                     /locus_tag="PLC06_09795"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HRO90779.1"
                     /translation="MNAEDDLLVTPTDSNRAWRFDLVLPVLLRPRATLARIVANSHPT
                     WRTPLVLLMLAAAGNALAAGTIKAAAAAAGEITFPPGFEYYTPEQQAQFQQAATATNN
                     TTFNYLLPALGAVLSVIIVWFLIGWLMHLIQTLFGGRGTSRASLDIAAWSGLPLLVRY
                     VVQIVVMLTSDQMIAGAGLSGFAPAGEGLGNALLAGILSHIDIYLIWQVVLLYLGLRL
                     SSQLPRAKGWLTVLLTVIIMLALRALPAALLAQFGGLTVIQPFF"
     gene            4789..5586
                     /locus_tag="PLC06_09800"
     CDS             4789..5586
                     /locus_tag="PLC06_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90780.1"
                     /translation="MDTTRNNGAAAVDDAKVENGTSPTGAAASFIATSGLRKSFQMGT
                     TMVHALAGVDMTIEAGTFQSIMGPSGSGKSTLLYLLGGLDRPTAGRIIVGGHALEQLD
                     ENALATYRRRFVGFIFQSFNLIPSLSAWENVAFPMRFAGIARGERQRRAFELLERVGL
                     ADRRNHKPTELSGGQQQRVAVARSLVNEPRLILADEPTGNLDTTSGQSIMDLLAELHR
                     DGRTVLVVTHDPRMAAYATHQIFLLDGCVVSEEKYREVAGVAPIGAA"
     gene            5624..>6937
                     /locus_tag="PLC06_09805"
     CDS             5624..>6937
                     /locus_tag="PLC06_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HRO90781.1"
                     /translation="MSNRHELTSKLVFRLAWLAVVALLAILIAVPALAQPPTGTPAAT
                     GQPAAGALTITAVQPGTLINDSEVEIIITGAGFVDGTVVVIDNFGGLQTAFVSANVLR
                     AIVPPGLPAGQYSVRVVNPDATTAEAPNALQIVLPAGPTATPAPSSTPAPTAFVRPLL
                     VVSSYGASAPQITPGQNLDFEMTIANAGQATATNVVATFTSGDFIARDTGGVRALGNL
                     APGQTARFWQPLFATTDLRGRSTAVLKVVATYTDVNGQSYDSTFELTFPVVPSGGGGG
                     PAATATPTPTTTPTPGPRQRPQLLVTSNETDPAKLQPGNQFVLNLTVENLGEANARNV
                     TLILGGGDSSSPTVDGTPVPGGGLAGAGGSFTDFAPIGSSNISRLGNLAVGESRPAAL
                     QLIVNTTTKAGAYPLKVSFVYTDDKGAGYVDDQVITLLVYQSPQVE"
BASE COUNT         1112 a         2452 c         2165 g         1208 t
ORIGIN      
        1 tggccgcggc gacggcccgc cccggggaga gcatcaatct gcgcctctac tggacatccg
       61 acggtaacat aagtcagtcc tacaaggtgt tcacccatct gctgggtgac gtgttcaacg
      121 ccggcggcgg caacttcctc tgggggcagg tcgacaacga gccggcggcc aacacccggc
      181 cgacgaccac ctggcgcgcg ggcgaggtca tcgtcgatga gtacgccatc cccatcgcgc
      241 cggacgcgcc gtcggggaca taccggatcg agatcggcct gtacgatccc atcaatggac
      301 aacggctgcc ggcgcttggc ccggatggcg caccgacagc cgatcatctt gttctcatca
      361 ccgttgatat tgagtgacgc cgtcgcttgc gagtgcgccc accaaagctg aatttgacga
      421 ttgctgagag gccatgcggc gagcaagagg atcagctatc cttgagggaa catgctgaga
      481 cgactcattc ccttcctgtt ggccgccgtg ctggttgctt gtgaccgctc accggccgcc
      541 acaccggccg ccaccctgcc cgtcgcgccg gcggagagcg cagccggtca ggcggttccg
      601 gcaaagacag ccggcctgac agccgagccg ggtctacccc cgacggccgc cgtctttgtg
      661 ccgttgctgc ccagcggcgg cacacaaccc accgcgacac cgccaccgac agtcatgccc
      721 accgaagcgc cgaccgatga agcgcccgtg gccccgaccg ccacgcccta cgtcagcaac
      781 atccccgatt tcacgcctcg ccccgaattg gggccgagca agatgggcat ccatgtggcc
      841 cggcccaaca gcgaggcgat catggagttc gttcgcgccg cccacccggc cgtggtcaag
      901 ggggttgacg acctcggctt tctggccgag gtgaaggccg tttcgccccg gacggtgacg
      961 gtcggccgct tctcggtgga tccacaggac tacgacggcg agccggaagc ggccgcggcg
     1021 gctctggtgg ccaggcaact gccgcagatg ttgctcaacc cggctgtcga ttactgggaa
     1081 ggctggaacg agccggaccc caatctcgac cggatggact ggtacgcccg gttcgaagcc
     1141 gagcgcgtgc gccaactggc ccaacacggg ctgcgcgcgg ccgtgggcgg cttctccgcc
     1201 ggcgtgcccg agctgaacga gttcgagttg ttcgtccccg ccatcgaaga agcacagcgc
     1261 cacggcggca tcctgaccct gcacgaatac gccgcgcccg acatcacctt cctgtatggc
     1321 gacccgttgc ccggttatcc gacatacccc gatcgcgggc cgctcatgtt tcgctatcgc
     1381 tggttctacc gcgacatact ggagccgcgc gggctggtcg tgccgctggt catctccgag
     1441 gcggggatcg acggcatact gggcaatcgg ccggggccgg acggattggg ctggcaggat
     1501 tttcaggcgt tctgggtgga gcagggctgg gggccggacg ggcagacggc cttcctcaat
     1561 cagttgcagt gggtggacaa tgaggcccga caagacccct acgtgatcgg ctacgccctg
     1621 ttcaccgccg ggggcgcccg cgcctggacg gcctatgaac tggccccgtt cctgccgcgt
     1681 ctggccgaat atatcgtcag tcaaaagcag tagcgcagac attcctgtct gcgagaggac
     1741 gcaatcacga acgattgcgc tacaatccgg ctcaatgtcg agagcgaatt acaaacattg
     1801 aggcgaagcg gcggcccggc ggccattgct tcgcccaaac tccatcaact atccgtgtcc
     1861 acttccaccc ccgaaccatc cacccgtcga ttcatgctga tcgccgccgc catcatgctg
     1921 ctggctctgg cgctgcgcct gcgcgggctg gacacgaccg ccctgtgggg cgatcaggcg
     1981 ttcacgctca acgccgccat gcgctgggtc aacggcggcg acatgccact ggcggccaac
     2041 aagtcgtcgg tcggcttcgt caacccgccg atgatcgaat atctctatgc ggcggccctg
     2101 tggctgtggc gcgacatcct gagcgtctcc tggctgacgc tgctgggcgg gctggcggcc
     2161 gtcgccgtca ccggcttcgt caccgcgcgc ctcttcggcc gtcgcgccgg gttgtgggcc
     2221 atgctggcct tcgccgtcgc gccctgggcc gtctactgga gccagctcat ctggaaccag
     2281 acgatggtgc cgcccttcgc ggcattggcc ctcggcgggc tgctgctcta cctggccgat
     2341 cggccgcgat ggcattatct ggtcatcagc ttcgtcgccg ccgccatcat gacccaggtg
     2401 catcccggct cggccgtcca gttggtgact attgccctgg cgctgctgct cttccggtgg
     2461 cgcgtgcgat ggcgacacgt gctggtcggg gcgctggcgt tcgccgccct ctatctgccc
     2521 tatctcgtct accagatcgg caccggctgg gccgatctcg gcgctatggg cgacatagcc
     2581 gggcaggagt cggccttcag cggcgcggcg gcgctgctca gcctcgacct gatccgcgcc
     2641 cagggcttgc ttcgcgccgt gccgggggtt gtcgccttcg acaatttggc gacggccctg
     2701 ttcctggcag cgctggggtt ggtggtgtgg cgcttgtggc gcaatgccgg ccggcgcctc
     2761 ccggattcac cccattccac cccggactca ctcccacagc catcggagcg gatcgccctc
     2821 gcggtcctgc tgctatggtt cttcatgccg ctgctgtttt acctgcgctc ttcggtctac
     2881 ctgcagaact actatctcct cagccaatgg ccggcccact tcatgatcct cggcatcggc
     2941 ctggacacgg cccggcgcgc ggccgcggga atggcggcgc aagccacgag acccgccaaa
     3001 cgacaggcct gggcagtggt cgggctaata ctgccggcgc tctttctgct ctcggccgcg
     3061 tatcaggtgt tcttctcgct gcgctatcag gacgcgcgcg ccgcggggca tgactcgctg
     3121 atgcaggtgc gccacgcgcg agcgatgatc gccatgtcgc gccggctgct gacggagcat
     3181 gagggttggc ggctggtggg cctgggtcat ggccatcagg cggacaactc cgacttggcg
     3241 ctgctgcaag agttcgtcga tccccggcgc gttctgctgg ccgacagcga gttggcgctg
     3301 cccctgccgc ggccctatgc cttcctgttg aacacccggc ccggcagcct ggccgcatac
     3361 accttgaacg acctggccta tcctgtgccg gacgcaaata tccaggtcgg ggacgccaca
     3421 tggcaattct acgaatggac agagccggag cagggcacaa gcagcccacc ggcggcgagc
     3481 tgggcgctgg ggcttgacct ggccggctat ttccccggcg aattcgttcc ggggcaagag
     3541 acgatcgtcg tcctcacctg ggacatcaat ggcccaccgc cggatgctct ctatcacttc
     3601 ggcgtctacc tgttgaacga gcgggatgaa gtcgtggccc agcatgacgg ccccggcttc
     3661 gacagcgccc agtggcgcgc gggcgaccgg ttcatcacct atcaccgcct ggccctgccg
     3721 cccgacctgc ctccgggaac ctatcgcgtc gccgcggcgt tatatagctg gcccgatctc
     3781 gttcgcgccg agctgcgcga cggcggcaac accgcctatt tgcaaacgat cacgatcagc
     3841 gctccctaag cccatggcct gagggccgat tggagtagct gctcgatgag cggctatggc
     3901 aagttgccgt cctatcggct atagtggata agctagggat tatttcgggc tgccctgaaa
     3961 tcgaagcggt aatacgatat tgttatgaac gcagaagatg atctcctcgt aaccccaacc
     4021 gactccaacc gcgcctggcg tttcgacctg gtgctgccgg tgcttctccg cccgcgcgcc
     4081 acgctggccc gcatcgtcgc caattcccat cccacatggc gcacgccgct ggttctgctc
     4141 atgctggccg ctgccggcaa cgcgctggcc gccgggacga tcaaggccgc cgcggccgcc
     4201 gccggcgaga tcacctttcc gcccggcttc gagtattaca cgccggagca acaggcccag
     4261 tttcagcagg cggccacggc caccaataac acaaccttca actacctgct gcccgccctc
     4321 ggcgcggtgc tgagcgtcat catcgtctgg tttctcatcg gctggctgat gcacctcatc
     4381 cagacgctct tcggtggccg gggaaccagt cgagcctcgc tcgacatcgc cgcctggtcg
     4441 gggctgccgc tgctggtgcg ctatgtcgtg cagatcgtcg tgatgctcac gtccgaccaa
     4501 atgatcgccg gggcgggcct gtcgggcttc gcgcccgcgg gcgagggtct cggcaacgcc
     4561 ctgctggccg gaatcctgtc ccatatcgac atttatctca tctggcaagt cgtgctgctc
     4621 tatctcggcc tgcgcctgag cagccagttg ccacgcgcaa aaggctggct gacggtgctg
     4681 ttgaccgtga tcatcatgct ggccctgcga gcgttgcccg ccgcgctgct ggcccagttc
     4741 ggcggcctga cggtgatcca gccgttcttc taggcgattg cccttcatat ggacaccacc
     4801 cggaacaacg gagcggccgc cgtagacgac gcgaaggtcg agaacggaac atcgcccacc
     4861 ggcgcggccg cgtcgttcat cgccaccagc gggctgcgca aatcgttcca gatgggcacg
     4921 acgatggtcc acgcgctggc cggcgtcgat atgaccatcg aagcgggcac gttccagtcg
     4981 atcatggggc cgtcgggttc cggcaaaagc accctgctct atctgttggg cgggctggat
     5041 cggccgacgg cggggcgcat catcgtcggg ggtcacgccc tggagcagct cgacgagaac
     5101 gcccttgcca cctatcgtcg ccgcttcgtg ggcttcatct tccaatcttt caacctgatc
     5161 cccagcctca gcgcgtggga aaacgtcgcc ttcccgatgc gtttcgccgg catcgcgcgc
     5221 ggcgagcggc agcgccgggc gttcgaactg ctggagcgcg tggggctggc cgaccggcga
     5281 aaccacaagc ccaccgagct atcgggcggg cagcagcagc gcgtcgccgt cgcccgctcg
     5341 ctggtcaacg agccgcgcct cattctggcc gacgaaccga ccggcaatct ggacacgacc
     5401 agcggccaga gcatcatgga cctgctggcc gagctgcacc gcgacggccg caccgtcctg
     5461 gtcgtcaccc acgacccgcg catggcggcc tatgcgaccc atcaaatctt cctgcttgac
     5521 ggctgcgtcg tcagcgagga aaaatatcgc gaagtggcgg gagtcgcccc aatcggggcc
     5581 gcataacggt cgccgaccgg cctgctcccg cttgagataa gttatgagta atcgtcacga
     5641 attaaccagc aaactcgttt tccgcttggc atggctggcg gtcgtcgccc tgttggcgat
     5701 tctgatcgcc gtgcccgctc tggcccagcc accgaccggg acgcccgccg ccaccggcca
     5761 acccgcggcc ggcgcgctca cgatcacggc cgtgcagccc ggtaccctca tcaacgactc
     5821 cgaagtggag atcatcatca ccggcgccgg ctttgtcgac ggcacggtgg tggtcatcga
     5881 caatttcggc gggctgcaaa ccgccttcgt cagcgccaac gtgctgcgcg ccatcgtgcc
     5941 gcccggtttg ccggcgggcc aatacagcgt gcgcgtggtg aatcccgacg caaccacggc
     6001 cgaggcgccc aacgccctgc agatcgtcct gcctgccggg ccgactgcca cgcccgcgcc
     6061 gagcagcacc cccgcgccga ccgccttcgt gcggccgctg ctcgtcgtca gctcctatgg
     6121 ggccagcgcg ccgcagatca cgcccggcca aaatctggat ttcgagatga ctatcgccaa
     6181 tgccggacag gcgacggcca ccaacgtcgt ggccacgttc accagcggcg atttcatcgc
     6241 ccgcgacacc ggcggcgtgc gcgctctggg caacctggcc cccggccaga cggcccgctt
     6301 ctggcagccg ctcttcgcca caaccgacct gcgcgggcgc tcgacggccg tgctgaaggt
     6361 cgtcgccacc tataccgacg tcaacgggca atcctatgat tcgacatttg agctgacctt
     6421 cccggtcgtt ccgtcgggcg gcggcggtgg cccggcagcc acggccaccc ctacgccgac
     6481 cacgaccccg acccccggcc cgcgccagcg cccgcagctg ctcgtcacgt ccaatgagac
     6541 cgaccccgcc aaattgcagc cgggcaacca gttcgtcctg aatctgaccg tcgagaacct
     6601 cggcgaggcc aacgcgcgca acgtcacgct cattctgggc ggcggcgaca gcagcagccc
     6661 caccgtcgac ggcacgccgg ttccgggcgg cggcctggcc ggcgccggcg gctcgttcac
     6721 cgatttcgcg cccatcggct cgtcgaacat cagccgcctg ggaaacctgg ccgtgggcga
     6781 gagtcgtccg gccgcgctgc aactcatcgt caacaccacg acgaaagccg gcgcctaccc
     6841 gctgaaggtt tcgtttgtct acaccgacga caaaggggcc ggctacgtcg atgaccaggt
     6901 gatcaccctg ctcgtctacc aatcgccgca ggtggaa
//
LOCUS       DAOMQG010000162         6909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4700_length_6909_cov_11.426466, whole genome shotgun sequence.
ACCESSION   DAOMQG010000162 DAOMQG010000000
VERSION     DAOMQG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_6909_cov_11.426466"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1774)
                     /locus_tag="PLC06_09810"
     CDS             complement(143..1774)
                     /locus_tag="PLC06_09810"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRO90782.1"
                     /translation="MSKKFLSMTFLLLFAGLAACSSGSTSGSSASADAASAVTAATDA
                     PEVADEIAEVSAPASSEADDDVARPDGWTEETHSNDVDPNYSVVFPDDKVNTITITID
                     PADWEAMQADMTEILGEPGTGGPMGGPGGMPGEGSEGMEPPTGAPEGARPGGPGGFGG
                     AGGPGGGDMVSETPMWVPATIEMDGNVWNHVGVRYKGNSSLTSAWNSGSLKMPLKFDF
                     DEFEDDYPEIDNQRFFGFKQLSLANGFSDDTYLRETLTYDLMRDAGLVAGNTAFYEVV
                     LDYGEGPVNLGIYTVVEVVDDTVIETAFGDDDGNIYEGDGAGVTLAEGTFDQIADSFE
                     KENNEDAADWSDIEDLYNVLHAETRLTDAAQWRADLEAVFDVDTFLNWLAVSAVVKNW
                     DTYGSMTHNFYLYNNPETGQLVWIPWDLNMTFGVGAGMGGGRDEQAGRPGGMGGRGNV
                     SFDKAEVDESWPLIRFLLDDPVYNEAYMGYLAAASGLFDADALNARITQMAEMLQPYI
                     VAAGENERFATALEQLRTAVSEQDAAVADFLATGP"
     gene            complement(1777..2400)
                     /locus_tag="PLC06_09815"
     CDS             complement(1777..2400)
                     /locus_tag="PLC06_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRO90783.1"
                     /translation="MSSDLFYLLLGAGLNLLVAVLIVRFIYYPVTQDKIYVFTFLAFN
                     TVIYFVMGLLTSAELSIGVGFGLFAIFSVLRYRTDEMPIREMTYLFIVIALPVMNSVL
                     MSDSNIARLLIANGVIVALLYALEKEWGFHFSASRIITYDRVDLITPANQEKLLADLR
                     ERTGLAIDRVEVRKINFLNDTVDMTIHYDKPRAPKNIPTLVPVNERG"
     gene            complement(2390..3253)
                     /locus_tag="PLC06_09820"
     CDS             complement(2390..3253)
                     /locus_tag="PLC06_09820"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRO90784.1"
                     /translation="MIETSENRNNRIAHSPEAVDVSLDTVWWPAPEPEHAAQLARFGS
                     IDLEQMAGAALLNRVETKYVFHEQTLDIILAALLDSYRVLEVNGNRLNHYRTLYYDTP
                     DFALYHRHHAGGRNRYKVRGRSYLDSDKSFLEVKRKVKENRTIKSRVRTAGLAEQLTP
                     PMDGFVQAHLPPGSPRLEPKLWNEYTRITLVGLGEPERVTIDLNLRVRVGDQTIIWPG
                     VVVAEVKTGGNDHDSVFKQLMKARGMRATSFSKYCIGVSMLYPEIKHNRFKPKLRMIG
                     QLIQKERNYVI"
     gene            3605..>4053
                     /locus_tag="PLC06_09825"
     CDS             3605..>4053
                     /locus_tag="PLC06_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRO90785.1"
                     /translation="MIKAGIYGATGYTGYELVKILQRHPAAEIAFTTSQSHAGKTLRE
                     VFPGAPALPLVAGDDAPLDDVDVAFLCLPHAAAAGTAMIALDAGARVIDLSADFRLRD
                     AATYAHWYNHIHPAPELLGDAVYGLTEVYRADLPGARLVANPGCYPT"
     assembly_gap    4054..4153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4194..5021
                     /gene="argB"
                     /locus_tag="PLC06_09830"
     CDS             4194..5021
                     /gene="argB"
                     /locus_tag="PLC06_09830"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRO90786.1"
                     /translation="MTRVIKIGGNEMNTPGFLEELARQIGGLAAATGEPLVVVHGGGQ
                     EIATLQTRLGIEPVKVDGLRVTDAESLAVAQMVLSGHTNKAIVKAFLAAGLDAAGFSG
                     IDGGLLRCRKKQHPTIDLGLVGEITGVRARFLNHFAMQGIIPVISPISLGEDGQTYNV
                     NADEAAGAIAAALGAEHLDFVSNVPGVMEDGRLLSQLTAPQANALIDDGIINGGMIPK
                     VRTALEALERGVPRVRIVNLAGLKGGGGTVFLSEPASASLTPRPDRWATIPLVTPGC"
     gene            5121..6302
                     /locus_tag="PLC06_09835"
     CDS             5121..6302
                     /locus_tag="PLC06_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRO90787.1"
                     /translation="MNKQDIIQDEAHYLMQTYKRADIVLERGEGVYLYDSDGRRYLDF
                     TSGIAVAALGHSDPDVAAAIADQARTLTHVSNLYYTAPQIALAKRLVDHSFADRVFFT
                     NSGTEAIEGALKFARKRARKQFGEGKHEIVAFNGGFHGRTMGALSITYKSIYREPFAP
                     LVPGVHFAPFNDLAAARDLIGPATCAVVVEPVQGEGGIHPATPEFLRGLRALCDEHGA
                     TLIFDEIQCGLGRTGHLWAHEASGVTPDIMTLAKPLANGLPIGAILMIEDVASAVGYG
                     EHGSTFAGGPLATRAACVVFDRVSQPAFLAAVAENGAYLRERLEEINSPHIREVRGAG
                     LLVGADLDIPAARVIAAARERGLLVINAGETTLRIAPPLIITPTHIDEAVAILHDSLA
                     A"
     gene            6422..6904
                     /locus_tag="PLC06_09840"
     CDS             6422..6904
                     /locus_tag="PLC06_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRO90788.1"
                     /translation="MTAADWPAVRAIYEEGMATGNSTFETSSPEWTAWDAAHRPDCRL
                     VARRDGRVIGWTALTPVSNRLVYAGVAEDSIYVAASARGRGVGRALLAALVDASEAAG
                     VWTLQAGIFPENAASLALHLACGFQVVGRRQRISFHQGVWRDFMLLERRSTCIGMDEG
                     "
BASE COUNT         1338 a         2241 c         1938 g         1292 t
ORIGIN      
        1 ccaatcgcaa cgatgattgg cagccggccg tcgtcgtgcg ccacgagccg ccgggcatct
       61 gggtgcgaac ggatgacggc cgtgaatggt tcatgaccaa cacctttcgc atccgtccgg
      121 cagctccgga caccggacgc gattaaggcc ccgtcgccag gaaatcggcg accgccgcat
      181 cctgctccga tacggccgtc cgcaactgct ccagcgccgt ggcaaaccgc tcattctcgc
      241 ccgccgcgac gatatacggt tgcagcatct ccgccatttg cgtgatgcgc gcgttcaggg
      301 cgtcggcatc gaacaacccg gatgcggccg ccagataccc catgtacgcc tcgttgtaaa
      361 ccggatcatc cagcaggaag cgaatgagcg gccagctctc gtccacttcg gccttgtcga
      421 acgaaacatt gccgcggccg cccatgccgc ccggccggcc cgcctgctcg tcgcggccgc
      481 cacccatgcc cgcgcccacg ccgaacgtca tgttgagatc ccaggggatc cacaccagtt
      541 ggccggtctc cgggttgtta tagaggtaga aattatgggt catcgagcca taggtgtccc
      601 agtttttcac cacggcgctg acggccagcc agttcagaaa cgtatcgacg tcgaaaacgg
      661 cctcaagatc ggctcgccat tgggccgcgt cggtcaaccg cgtttcagcg tggagtacat
      721 tatagaggtc ctcgatatcg ctccagtcgg ccgcgtcttc gttgttttcc ttctcaaaac
      781 tgtcggctat ctggtcaaag gtgccctcgg ccagagtgac ccccgcgccg tcgccctcat
      841 agatattgcc gtcatcatcg ccaaacgcgg tttcgatcac cgtgtcgtcg accacctcaa
      901 ccacggtata gatacccagg ttgaccggcc cttcgccata atccaggacc acctcgtaga
      961 acgcggtatt cccggccacc agtccggcgt cgcgcatcag atcataggtc agcgtctcgc
     1021 gcagataggt gtcgtcgcta aacccgttgg ccagcgatag ctgcttgaag ccgaagaacc
     1081 gctggttgtc gatctcggga taatcatcct cgaactcgtc aaagtcgaac ttgagcggca
     1141 tcttgagcga gccactgttc cacgcgctcg tcaacgacga attgccctta tagcgaacgc
     1201 cgacatgatt ccagacattg ccgtccattt caatcgttgc aggcacccac atgggcgtct
     1261 cgctgaccat atcaccgcca ccagggccac cagcgccacc aaaaccaccg gggccgccgg
     1321 gccgggctcc ttccggcgct ccggtcggtg gctccatgcc ttcagaccct tctccgggca
     1381 ttccgcctgg gcctcccatc ggcccgcccg tacccggttc gccgagtatc tcagtcatat
     1441 cggcctgcat cgcctcccaa tcggcgggat cgatagtaat agtgatcgtg ttgaccttgt
     1501 catcaggaaa gacgactgaa taattcggat ccacgtcgtt gctgtgcgtc tcctctgtcc
     1561 agccatccgg ccgggcgacg tcatcgtcag cttcagagga agccggcgcg gagacctccg
     1621 caatttcatc ggccacttca ggcgcatccg tagcggctgt gacggcgctc gccgcgtccg
     1681 ccgatgcgct gctcccactc gtggagccgg acgaacaggc ggccagccct gcaaacagca
     1741 acaagaacgt catgctcaga aatttcttgc tcacagttac cccctttcat tgaccggaac
     1801 gagcgtcggg atattcttcg gcgcgcgtgg tttgtcgtaa tggatggtca tatcgaccgt
     1861 atcgttcagg aaattgatct tgcgcacctc gacgcgatcg atcgccagcc cggtgcgctc
     1921 gcgcagatcg gccagcaact tctcctgatt cgcgggcgtg atgagatcca cgcgatcata
     1981 ggtgatgatc ctgctggcgg agaaatgaaa cccccattct ttctccagcg cgtagagcag
     2041 ggcaacgatc acgccgttgg cgatcagcaa tcgcgcgata tttgaatccg acatcagcac
     2101 agagttcatt accggcaagg cgatgacgat gaacagatag gtcatctcgc gaatgggcat
     2161 ctcatccgtc cggtaacgca ggacagagaa aatggcgaac aagccaaagc cgacgccgat
     2221 actcaactcg gcgctggtca gcaatcccat cacgaagtag ataaccgtgt tgaaagccag
     2281 aaacgtaaag acgtaaatct tgtcctgcgt gacagggtaa taaataaatc tcacgattag
     2341 caccgccacc agcaggttca ggccggcgcc caggagcaaa tagaacaaat cagatgacat
     2401 aatttctctc cttctgaata agttggccga tcatccgtaa tttgggctta aagcgattgt
     2461 gtttaatttc ggggtagagc atcgacacgc cgatgcaata tttactgaaa ctggtagctc
     2521 gcatgccgcg ggctttcatg agttgcttga agactgaatc gtgatcgttg ccgcccgtct
     2581 tgacctcagc cacgacaact cccggccaaa tgatcgtttg gtctccgaca cgcacccgca
     2641 gattcaggtc gatcgtcaca cgctccggct cgcccaaccc aaccagcgtg atgcgggtgt
     2701 attcattcca gagcttcggc tccagacgcg gcgaaccggg cggcaagtgc gcctgaacga
     2761 atccatccat cggcggcgtt aactgttcgg caagcccggc cgtcctgaca cggcttttga
     2821 tcgttcgatt ctcctttacc tttcgcttca cttcgagaaa cgacttgtcg ctgtcaagat
     2881 aggaacggcc gcgaaccttg taccggttgc gcccaccggc gtggtggcgg tgatacagag
     2941 cgaaatcagg cgtgtcgtaa tacaacgtcc ggtaatgatt cagccggttg ccattcactt
     3001 caaggacgcg ataggagtca agcagcgcgg ccagtatgat gtcgagggtt tgctcatgga
     3061 agacatactt cgtctccacg cgatttagca gagcggcacc cgccatctgc tccagatcaa
     3121 tcgacccaaa ccgggccagt tgcgccgcgt gttccggttc gggggcaggc caccagactg
     3181 tatccagact cacatcgacc gcttccggcg aatgggctat tctgttattt ctgttttcgc
     3241 ttgtttcaat catatcttca ctacctccgg gaacgtcgcc gatacgaaca tcgggcgttt
     3301 ccccttaatt gatctcaagt ttgccccaca aatgttagga aaatgttcag gaaggtttcc
     3361 gatgttgtaa atcccggcga tttctcattc ggcggaataa aactattgac attcttctct
     3421 cgattgttat cattcttccc atgcacgttt acacggctca ggctttcccg cgtcgccctc
     3481 gcccgccccg gaccacaggt ctggtgcttt tggttgcctg agtcgtcgat aaccgacacg
     3541 aatgaaccga ccgccagacc tcaaaagggt ctggcggttt tttgttgagt ctgaggtaca
     3601 tcacatgatt aaagccggca tctacggcgc gacaggctac acgggatacg aactggtgaa
     3661 gatactccag cgccatcccg cggccgagat cgctttcacc acatcgcaat cgcatgcggg
     3721 caagaccctg cgcgaggtct tccccggcgc gccggccctg ccgctggtgg ccggcgacga
     3781 cgcgccactc gacgacgtag atgtcgcctt cctctgcctg ccccacgccg ccgcggccgg
     3841 gaccgccatg atcgcgctgg atgccggcgc gcgcgtcatc gacctgagcg ccgattttcg
     3901 cctgcgcgac gccgcgacct atgcccactg gtacaaccac atccatcccg cgccggagct
     3961 gctcggcgat gccgtctatg gcctgaccga ggtctatcgc gccgacttgc cgggggcgcg
     4021 gctggtcgcc aaccccggct gctatcccac gagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnccgagac ggaagggctg gcctgattga caggaacaac atcatgacac
     4201 gagtgatcaa aattggcggc aacgagatga atacgccggg ctttctggaa gaactggccc
     4261 gacaaatcgg cggattggcg gccgcaaccg gcgagccgct ggtggtcgtc catggcggcg
     4321 gccaggagat cgccacgctc cagacacgcc tgggcatcga accggtgaag gtcgacggcc
     4381 ttcgcgttac cgacgccgaa tcgctggccg tcgcccagat ggtcctgtcg gggcatacca
     4441 acaaggccat cgtcaaggcg tttctggccg ccgggctgga cgcggccggc ttcagcggca
     4501 tcgacggcgg cctgttgcgc tgccgcaaga aacagcatcc gaccatcgac ctgggcctgg
     4561 tgggcgagat caccggcgtg cgcgcaaggt tccttaacca tttcgccatg cagggcatca
     4621 tccccgtcat ctcccccatc tctctgggcg aggacggcca gacctataac gtcaacgccg
     4681 acgaggccgc gggagccatc gccgcggcgc tgggggccga acatctggac ttcgtatcca
     4741 acgtccccgg cgtcatggaa gacggccggc ttctgtcgca actgaccgcg ccgcaagcca
     4801 acgcgctcat cgacgacggc atcatcaacg gcggcatgat ccccaaggtc aggacggctc
     4861 ttgaggcatt ggagcgcggc gtgccgcgcg tgcgaatcgt caacctcgcg gggctgaaag
     4921 gcggcggcgg caccgtattc ctgagtgaac cggcttccgc gtcattaaca cccagacctg
     4981 acaggtgggc gacaataccg cttgttacac caggatgtta atcgcccacc tgtcaggtct
     5041 ttgctccgaa aaacgcaacg aaagttgcga actacaagga aagaaatcat acgcaatcgt
     5101 gcaggtacgt tgacaagatc atgaataaac aagacatcat tcaagacgaa gcgcattatc
     5161 tgatgcaaac ctacaagcgg gccgacatcg tgctggaacg cggcgagggc gtctacctct
     5221 acgacagcga cggccgccgt tacctcgatt ttacgtccgg catcgccgtc gccgccctgg
     5281 gtcacagcga ccccgacgtg gccgcggcca tcgccgacca ggcccgcacc ctgacccacg
     5341 tcagcaacct ctactacacc gcaccccaga tcgcgttggc caagcgtctc gtcgatcact
     5401 ccttcgccga ccgggtcttc ttcaccaact ccggcaccga ggccatcgag ggcgcgctca
     5461 aattcgcccg caagcgcgcc cgcaagcagt tcggcgaggg caagcacgag atcgtcgcct
     5521 tcaacggcgg cttccacggc cgcacaatgg gcgcgctctc gatcacctac aagtccatct
     5581 atcgcgagcc gttcgcgccg ctcgtccccg gcgttcattt cgcgccgttc aacgacctgg
     5641 ccgccgcgcg cgacctgatc ggcccggcca cgtgcgccgt cgtggtcgag ccggtgcagg
     5701 gcgaaggcgg catccacccc gccacgccgg agttcctgcg cggtctgcgc gccttgtgcg
     5761 acgagcatgg ggcgacgctc atcttcgacg agattcagtg cggactcggc cgcaccggcc
     5821 acctgtgggc gcatgaggct tcgggagtta cacccgacat catgaccctg gccaaaccgc
     5881 tggccaacgg cctgcccatc ggcgcgatcc tgatgatcga ggatgtcgcc tcggccgtcg
     5941 gctatggcga acacggcagc accttcgccg gcgggccgct ggccacccgc gccgcgtgcg
     6001 tggtcttcga tcgcgtctcc cagccggcgt tcctggccgc cgtcgctgaa aacggggcct
     6061 atctgcgcga acggcttgag gagatcaaca gcccccacat acgtgaagtg cgcggggcgg
     6121 ggcttctggt gggggcggac ctcgacatcc ccgcggccag ggtcatcgct gcggcccgtg
     6181 agcgggggtt gctcgtcatc aacgcggggg aaacgactct gcgcatcgcc ccgccgctca
     6241 ttatcacccc aacccatatc gacgaagccg tcgccatcct gcacgacagc ctggcagcct
     6301 gagatcacgc cgaggagact tagccatgaa cctggaccta cccctctcca actggttcac
     6361 ccgtctgccg aaccggcagc gaactgccgc tcgcccgaca gctccgacac ggatcgctcc
     6421 gatgactgcc gccgattggc cggccgtgcg cgccatctat gaggaaggca tggcgacagg
     6481 caactcgaca ttcgagacca gctcgccgga gtggacggca tgggacgcgg ctcaccggcc
     6541 cgattgccgt ttggtggccc gacgagacgg ccgcgtcatc ggctggaccg cgctgacgcc
     6601 ggtgtccaac cggctcgtct acgccggagt ggctgaggat agcatctacg tcgccgcgtc
     6661 ggctcgcggt cgcggcgtcg gccgcgcctt gctggccgcg ctcgtcgacg cttccgaagc
     6721 agcgggggta tggacactgc aagcgggcat tttccccgag aacgccgcca gccttgccct
     6781 gcatctcgcc tgtggcttcc aggtcgtcgg ccgccgccag cgtatcagct tccatcaggg
     6841 cgtctggcgc gatttcatgc ttctggagcg gcgcagcacg tgcatcggga tggacgaagg
     6901 ataaaaggt
//
LOCUS       DAOMQG010000163         6899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4708_length_6899_cov_9.547341, whole genome shotgun sequence.
ACCESSION   DAOMQG010000163 DAOMQG010000000
VERSION     DAOMQG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4708_length_6899_cov_9.547341"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1815)
                     /gene="groL"
                     /locus_tag="PLC06_09845"
     CDS             complement(160..1815)
                     /gene="groL"
                     /locus_tag="PLC06_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191209.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRO90789.1"
                     /translation="MSAKQLVFSEDARRKLKRGIDTVATAVSTTLGPKGRNVAIDKKF
                     GAPTVTHDGVTVAKEIELEDPFENMGAQLFKEAAVKTNDIAGDGTTTATVLAQHMVHE
                     GLKNVAAGANPMLLKQGIEIGTQALANKIRSMAISIDSFDEIASVASISAQDPEIGKL
                     IATVMDKVGKDGVITVEESKLLEFETEYVEGMQFDRGYISPYFVTDAEHMEAVIEDAY
                     ILIHDKKISSAQDMLPVLEKLVQIGRKNLVIIAEDVDGEALATLILNKLRGMINALAI
                     KAPGFGDRRKAMLQDIATLTGGQVITEEVGRKLDSVQLTDLGRAGKVVSTKDDTTIVD
                     GKGDEAAINGRVDQIKVEINNSTSDYDKEKLQERLAKLAGGVAVIRVGAATEVELKEK
                     KHRVEDALSATRAAVEEGIVPGGGVALLNALPALDDVKAPAGDISTGVNILRVALEAP
                     MRKIAENAGLNGDVIIQNVRRLQAEKKDPKLGYNVMTGEYPNMVKAGIIDPAKVTRGA
                     LENAASIAAMILTTEALVTDMPEDKKAPAMPGGGGMGMGDMDF"
     gene            complement(1842..2135)
                     /gene="groES"
                     /locus_tag="PLC06_09850"
     CDS             complement(1842..2135)
                     /gene="groES"
                     /locus_tag="PLC06_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRO90790.1"
                     /translation="MAMKLKPLGDRLVVEPRERESTTASGLVLPETAKEKPQEGEVIA
                     VGPGRRDEDGDRIEMDVKVGDIVLYAKYGGTEIKIEDKKLLILKESDVLAIVG"
     gene            complement(2374..3594)
                     /locus_tag="PLC06_09855"
     CDS             complement(2374..3594)
                     /locus_tag="PLC06_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO90791.1"
                     /translation="MIINRKPPRYPRRGPSCLLVIFVIFGIAVSLYIIQNAEEVRVAI
                     MPTPTSEPTRSATELAVLAELSRNDGANAEAIAFYDKAVELDPTNVEFYIRAIDLLVR
                     ENQPQRAVERAEQATILDPENDAVWTAAAAAYLAEGNRLSDLGDQNAANLQYANAAQA
                     ARKAININPNNATALAYAAGGTVLQGDPEKYEQAQVFADDAVRLEPENAIARYYMATV
                     FTYQGRYTEAMEQYQRGIEADPNNPNLHIGLAYNYYGTGSIPDALLSFEDAIAADPDN
                     AEAYDGRAYMYLQLGDAPLAEQNALEATRLNPNMARAQGRLGEAFFRQNNYAEAVEAL
                     EKAVAMYGRATNLNARFFNMLAQAYIRDDLNNCSLAVPLFEQVLNATANELILAASNE
                     GLVECRRASLGTSP"
     gene            complement(3685..4638)
                     /locus_tag="PLC06_09860"
     CDS             complement(3685..4638)
                     /locus_tag="PLC06_09860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO90792.1"
                     /translation="MMVSLIATVKNEGEALRPLLDSIIDQTRPLDEVVICDGGSTDST
                     LTILEEYRRWLPLRVIVAPGTNISQGRNRAIEAATGDVIAATDAGVVLSPTWMEDLAA
                     PFSEASVQVVSGWFEADPYTDFEVVMGATVLPSVEDVDPATFLPSSRSVAFRKSAWAA
                     VGGYPEWLEHSEDLVFDMALRDRFGPFAFAPNAVAYFRPRSNLRSYYRQYFAYARGDG
                     KANLWPKRHLIRYATYFLALPLLLRMIWRERWPGWMLLALGVGAYSRRPAERLWHNTW
                     GWRPPARARAFALIPIIRLVGDVAKMLGYPAGMWWRLRNRA"
     gene            4722..5279
                     /locus_tag="PLC06_09865"
     CDS             4722..5279
                     /locus_tag="PLC06_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HRO90793.1"
                     /translation="MDTNSLLTLLLHGNQLKRTARTGWGQRGVPAPENVAAHSYGVVF
                     AALLLAESMAEPLDLAAVLAMAALHDLPEGLTTDIPAPAWRFLPDGAKAAAELRAMEQ
                     IVGDAPIAPQLMAWWEQLRENSTPEAQLVHDADKIDIYLQAYIYERQTGNRQLEEFWT
                     KPHRFHFPKAQAVYDLLGRLRASGQ"
     gene            5398..6891
                     /locus_tag="PLC06_09870"
     CDS             5398..6891
                     /locus_tag="PLC06_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90794.1"
                     /translation="MNYRRFLLPFVLILLAGVALAEPIRLMAQTAEPAPATADPALLP
                     TPTLATSGTPQPGGSPIPRVHVVQEGENLTLIATDYGVTVEDILAINNLANGDVLAVG
                     QELIIPGGTGEAIATVHTVAPGDSLAGIAAGFNTTVADVIATNRLIGTNPPLIVGQTV
                     PVISRTGSAAGRLVTGRPYLVESGETLLMVAARFGLPLSALAEANQLPTNAAVFPGQR
                     LRIPDESSTYRFLPQGWIDVELSPLTATQGATLSIFVRNLQDGTPAGRFGDLPLLFAP
                     YEDGYAALVGIDGFTEPGIYDLELSGGDESGFWSPVRARVPLVEAAYDTQYIELGEAL
                     DGLLDPNLRATEDEFLKGIYAHFTEERRWDGPFQVPLTTTIISAPYGGRRSYNGGPIE
                     IYHTGIDYASAQGTTVMAPANGVVVFSDMLELRGGTIIIDHGMGVMTGYYHLSERLVE
                     VGQAVTVGEPIARVGTTGLSSGPHLHWDLRVMDVTVDPTPWTQRAFP"
BASE COUNT         1227 a         2081 c         2171 g         1420 t
ORIGIN      
        1 cgggcgcaag cgtaccatag acaaacagcc gattaatcat catctctcct tggcagccaa
       61 caaagcatat aatgcaaaag accccttggg caaggggtct ttgcggatcg tcgattagaa
      121 cccgagtttc tcggaagaaa ctcgggttct ggtgtcgaac tagaaatcca tatcgcccat
      181 acccatgccg ccaccgccgg gcatggccgg agccttcttg tcttcgggca tgtcggtcac
      241 cagcgcctcg gtggtcagga tcatggcggc gatgctggcc gcattctcca gcgcgccgcg
      301 ggtcaccttg gccgggtcga tgatgccggc cttgaccatg ttcgggtatt cgccggtcat
      361 gacgttgtag cccagcttcg ggtctttctt ctcggcttgc aggcgacgaa cgttctggat
      421 gatgacgtcg ccgttcaggc cggcgttctc ggcgatcttg cgcatcgggg cttcgagggc
      481 cacgcgcagg atgttgacgc cggtggagat gtcgcccgcg ggggccttca cgtcgtcgag
      541 cgccggcagg gcgttgagca gggccacgcc gccgccgggc acgatgccct cttccactgc
      601 cgcgcgggtc gcgctcagcg cgtcctcgac gcggtgcttc ttctctttca actcgacctc
      661 ggtcgccgcg ccgacgcgga tgacggcgac gccgccggcc agcttcgcca gacgctcctg
      721 cagcttctcc ttgtcatagt cggaggtgct gttgttgatt tcgaccttga tctgatcgac
      781 gcgaccgttg atggccgcct cgtcgccctt gccgtcgacg atggtggtgt cgtccttggt
      841 gctgacgacc ttgccggcgc ggcccagatc ggtgagctgg acgctatcca gcttgcggcc
      901 gacttcctcg gtgatcacct ggccgccggt cagggtggcg atgtcttgca acatggcctt
      961 gcggcggtcg ccaaagccgg gggccttgat ggccagggcg ttgatcatgc cgcgcagttt
     1021 gttgaggatg agggtggcca gcgcctcgcc atcgacgtct tcggcgatga tgaccaggtt
     1081 cttgcggccg atctgcacca gcttctccag cacgggcagc atgtcctggg cggagctgat
     1141 ctttttgtcg tggataagga tataggcatc ctcgatgacc gcttccatgt gctcggcgtc
     1201 ggtcacgaag taggggctga tgtagccgcg gtcgaactgc ataccttcga catactcggt
     1261 ctcgaactcc agcagcttcg attcctcgac ggtgatgacg ccgtccttgc cgaccttgtc
     1321 catgaccgtg gcgatcagct tgccgatctc cgggtcctgg gccgagatgg aggccaccga
     1381 ggcgatctca tcaaagctgt cgatggagat ggccatgctg cggatcttgt tggccagcgc
     1441 ctgggtgccg atctcgatgc cctgcttcag gagcatcggg ttcgcgcccg cggcgacgtt
     1501 cttcaggccc tcatggacca tgtgctgggc caaaacggtg gcagtagtgg tgccgtcgcc
     1561 ggcgatgtcg ttggtcttga ccgccgcttc cttgaacagc tgggcgccca tgttctcgaa
     1621 cgggtcttcc agctcaattt ccttggccac ggtgacgccg tcgtgggtga cggtcggcgc
     1681 gccaaacttc ttgtcgatgg cgacattgcg gcccttgggg ccgagggtgg tggatacggc
     1741 cgtggcgacc gtgtcgatgc cgcgtttcag cttgcggcga gcgtcttcag agaatacgag
     1801 ttgcttcgca gacattttat tcttatctcc gttaattagt cctagccaac gatagccaga
     1861 acgtcagatt ccttgaggat gagcagcttc ttgtcttcga tcttgatttc cgtgccgcca
     1921 tacttggcat agagcacgat atcgccgacc ttcacatcca tctcgatgcg gtcgccatct
     1981 tcgtcgcgac ggccggggcc gacggcgatc acttcgcctt cctgcggctt ttctttggcg
     2041 gtctccggca aaacgaggcc ggaagcggtg gtggattcac gctcgcgggg ttcaacgacc
     2101 aggcggtcgc caagcggctt gagtttcata gccatgttac actatctcct ttgaaaccaa
     2161 acgatatgat tgtcaggttg taaaacggtg ggagttatct tttagcactc atgtagaatg
     2221 agtgctaatt ctcgattagc attgtagcag cagccggtgg agatgtcaag gggaacttag
     2281 cagtctgatc ggttgagtgc caattcgtga ccagcccctg taatttaccc caaaggcgcg
     2341 agaacgcaag agaaaacggc cacacctcta cgcctagggg gatgtgccca gtgacgcccg
     2401 ccggcattca acgaggccct cattggaagc ggcgaggatg agttcgttcg ccgtggcatt
     2461 cagcacctgc tcgaacaagg gaaccgccag ggaacagtta ttcaggtcgt cacggatata
     2521 ggcctgggcc aacatattga agaatcgcgc gttcaggttg gtggcgcgac cgtacatggc
     2581 gaccgctttc tccagcgctt cgaccgcttc cgcatagttg ttttgccgga agaaagcttc
     2641 gcccaggcga ccctgagcgc gggccatgtt ggggttcagc cgtgtcgctt ccagcgcgtt
     2701 ttgctcggcc agcggggcgt cgcccagttg caggtacata taggcgcggc cgtcatatgc
     2761 ctcggcgttg tcggggtcgg cggcgatggc gtcctcgaaa ctgaggagcg cgtcagggat
     2821 gctgcccgtg ccgtaatagt tgtaggccag cccgatatga agattgggat tgttggggtc
     2881 ggcttcgatg ccgcgctggt actgctccat cgcctcggta tagcgcccct gataagtgaa
     2941 gacggtcgcc atatagtagc gcgcgatggc attctccggc tccagtcgca cggcgtcgtc
     3001 ggcgaagacc tgggcctgct cgtacttctc cgggtcgccc tgcagcaccg ttcccccggc
     3061 ggcataggcc agagcggtgg cgttgttggg gttgatgttg atcgccttgc gtgccgcctg
     3121 ggcggcgttg gcatattgca aattggcggc gttctggtcg cccaaatcgc tgaggcggtt
     3181 accctcggcc agataggcag cagcggcggc cgtccacacc gcgtcgtttt cgggatcaag
     3241 gatcgtagct tgttcggccc gctccacagc tctctggggt tgattctcgc gcacgagcag
     3301 gtcgatggcg cggatataga actcaacatt ggttggatcg agttccaccg ccttgtcgta
     3361 aaaggcgatg gcctcggcat ttgcgccgtc gttgcggctc agttcggcca atacggccag
     3421 ctctgtggct gagcgggttg gctccgaggt gggcgtaggc atgatcgcca cgcgcacctc
     3481 ttcggcgttc tggatgatgt agaggctaac cgcgatgccg aagatcacga agataacgag
     3541 caggcacgaa gggccgcggc gcgggtagcg aggtggtttg cggttgatga tcatgattga
     3601 ttatcccgca tcatagcaac ggcccggcgc gcgggcaaac ctgctcactg gcgattaaga
     3661 tggtcgattg ggcgcaagga cggatcaggc gcgattgcgg agccgccacc acatccccgc
     3721 cgggtagccc aacatcttgg cgacatcccc tacgaggcgg atgatgggga tgagagcgaa
     3781 ggcgcgggcg cgggcgggtg gccgccaacc ccacgtgttg tgccacaggc gctcggccgg
     3841 ccgccgtgaa taggcgccga cccccagcgc cagcagcatc cagccgggcc accgctcgcg
     3901 ccagatcatg cgcagaagca gcggcagcgc caggaagtag gttgcatagc gaatgaggtg
     3961 gcgtttgggc cacaggttgg ctttgccgtc gccgcgggca taggcgaaat actgccgata
     4021 gtatgaccgc aggttggagc ggggacggaa ataagccact gcgttaggtg caaatgcgaa
     4081 cggcccgaat cgatcgcgca gggccatgtc gaacaccaga tcctcgctgt gctccagcca
     4141 ttccggatag ccgccgacgg ccgcccaggc gcttttgcgg aaggcgaccg aacggctgga
     4201 gggcaggaaa gttgccggat ctacatcctc gaccgaaggc aagaccgtcg cccccatgac
     4261 gacctcgaag tcggtgtagg gatccgcctc gaaccagccg ctgacgacct ggaccgacgc
     4321 ttcggaaaaa ggtgcggcga gatcttccat ccaggtgggc gagagtacca cgccggcatc
     4381 ggtggcggcg atgacgtcgc ccgtggccgc ctcgattgcc cgattgcgcc cctggctgat
     4441 gttcgtcccc ggcgcgacga tcacccgcag cggcagccat cgccgatact cttccaggat
     4501 ggtcaaggtg ctgtcggtcg agccgccatc gcagatgacc acctcatcga gtggtcgggt
     4561 ctgatcgatg atcgagtcga gcagcggcct aagcgcctcg ccttcattct tgacagttgc
     4621 aatcagggat accatcatag tgctatctag cttaaggaac actgcaacgg ccgattcgtt
     4681 gctttaagcg atcaattcga gagctttgct tacattcacc aatggacacg aattcattat
     4741 taaccctctt gttacacggc aaccagttga agcgaaccgc gcgcacgggc tgggggcagc
     4801 gcggagttcc cgcacccgag aacgtggccg cgcacagcta tggcgtggtc ttcgcggcgc
     4861 tattgttggc cgagagcatg gcggaaccgt tggacctggc cgccgtgctg gccatggctg
     4921 cgctccacga tctgccggag gggttaacga ccgacatccc cgcccccgcc tggcgtttcc
     4981 tgcccgatgg ggccaaggcg gccgccgaat tgcgggcgat ggagcagatc gtgggcgacg
     5041 cgcccattgc cccgcagctg atggcctggt gggaacaatt gcgagagaac agcacgccag
     5101 aggcccagct tgtccacgat gccgacaaaa tcgacatcta tctgcaagcc tacatctatg
     5161 agcggcagac cggcaaccgg caactggagg agttctggac aaagccgcac cgttttcatt
     5221 ttcccaaagc gcaggcggtt tacgatctgc tgggtcgcct gcgcgcttcc ggccaatgac
     5281 ggccggccta aaacgcccta accctgatcc tctgcccggc ccgaatgagc ctaattaccc
     5341 ctttctgagt cttaattggc aggctgggca tgatttggca gaatagcggg attatttatg
     5401 aattatcgac gatttcttct accgtttgtc ctcatcctgt tggcaggggt ggctctggcg
     5461 gagccgatcc ggttgatggc ccagacggcc gagcctgctc cggcgacggc cgatccggcg
     5521 ctcctgccca cgccaacctt ggcgacatcg ggaaccccgc aaccaggtgg ttcgcccatc
     5581 ccccgcgtcc atgtggttca ggagggcgaa aacctgaccc tgatcgccac agattatggc
     5641 gtgacggtcg aagatattct ggcgatcaat aatttggcca atggtgatgt gctcgccgtg
     5701 ggtcaggagc ttatcatccc cggcggcacg ggcgaagcca tcgccaccgt ccataccgtc
     5761 gctcccggcg attctctggc cggcattgcc gccggattta atacgaccgt cgccgacgtc
     5821 atcgccacga atcggctgat cggcaccaac cccccgttga ttgtggggca gaccgtgccc
     5881 gtcatcagcc gcaccgggtc ggccgccggt cgattggtca caggacgacc gtacctcgtc
     5941 gagtcgggcg aaacgctgct catggttgcc gcccgcttcg gcctgccgct cagcgcattg
     6001 gccgaggcca atcaactacc cacgaacgcc gccgttttcc ccggccagcg tctgcgcatc
     6061 cccgacgaaa gcagcaccta ccggttcctg ccgcaaggct ggattgacgt ggaactgtcg
     6121 ccgctcacgg cgacccaggg cgcaaccttg tcgatatttg tccgcaacct tcaggacggc
     6181 acgcccgccg gccgttttgg ggacttgccc ctgctgttcg cgccctacga ggacggctac
     6241 gcggctctgg tggggatcga tggcttcacc gagccgggca tctacgatct ggagctttcg
     6301 ggcggcgatg agagcggctt ctggtcgccc gtgagggcgc gcgtgccctt ggttgaggcg
     6361 gcctatgaca cgcaatacat cgagttgggt gaggcgctcg acggcttgct tgatcccaat
     6421 ctgagggcga ctgaggatga gttcctgaag ggcatctacg cccatttcac cgaggaacgg
     6481 cgatgggacg gccccttcca ggtgccgctg accacgacga ttatctctgc tccctacggc
     6541 ggccgtcgct catacaacgg tggaccgatc gagatttacc ataccggcat cgattacgcc
     6601 tccgctcagg gcacgacggt gatggctccg gccaatggcg tagtcgtctt cagcgacatg
     6661 ctggaactcc gcggcgggac aattattatc gatcacggca tgggtgtgat gaccggctac
     6721 tatcatcttt cggaaagatt ggtcgaggta gggcaggcag ttaccgtcgg agagccgatt
     6781 gcccgcgtgg gtacgaccgg cttgtccagc gggccgcacc tgcattggga cctgcgggtg
     6841 atggatgtga ccgtagaccc gaccccgtgg acgcagcggg cctttccata agattggca
//
LOCUS       DAOMQG010000164         6877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4725_length_6877_cov_11.440340, whole genome shotgun sequence.
ACCESSION   DAOMQG010000164 DAOMQG010000000
VERSION     DAOMQG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4725_length_6877_cov_11.440340"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..4111
                     /locus_tag="PLC06_09875"
     CDS             176..4111
                     /locus_tag="PLC06_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRO90795.1"
                     /translation="MSSIYTKSYARTTKPMELPTLIDVQLRSFEQFISEGLAELFDEI
                     SPIESFNGDLKLYFPSKRKEVADFNLTYWFGEPKYNVEECVERDMSYAAPLYVRVLLY
                     SQDLDQPIEQDIFMGDFPLMTSAGTFIINGTERVVVSQLIRSPGAYFEVEEERATGRP
                     LAMGKLIPDRGAWMEFETRKTDYITIKFNRKRTVPVTLFLRAMAAIPDGLGKPLISEG
                     TDEELLALFADIDTNGEHLYIQGSIEQEPHWDQEKSIPQQALIEFYKRMRPGDPPTLD
                     NASQYLQEQLYDSRRYDLAAVGRYKLNKRLNLQNEVPLDHRTITQQDIVNFVRHMILI
                     NNGQEGPDDIDHLGNRRVKTVGELIQAKLRVGLRRMERVVRERMSIRESDSVTPVALI
                     NIRPVVAAIREFFGSSQLSQFMEQANPLAELTHKRTLSALGPGGLRRERAGFEVRDVH
                     HSHYGRICPIETPEGPNIGLIGRLATYGQVNKYGFIETPYRRVMNKLPVGDALINHDV
                     FVNVTDPSTGEIIIAQGETVTAEHLNALREAGIQEVQVRPFLTEEVLYMSADEEDRYT
                     IAQANAQIDDRGQFVDARVSSRRNQQFRFTSVQRIDFMDVAPRQIVGVSAALIPFLEH
                     DDANRALMGSNMQRQAVPLLQPDVPLVSTGMELQAALNSGQVVLADQIGEVVSVSGDK
                     IVVMGDNGPRIYNLRKYNRSNQSTCIDQHPVVQRGQRVQIGDVLADSSSTHYGELALG
                     QDVVVAFLSWEGGNFEDAILVSEAMVRRDRFTSIHIEKHEVEARDTKLGPEEITYDIP
                     NVSEDALKDLDERGIIRVGADVNENDILVGKITPKGEKELSPEEKLLRAIFGDKAREV
                     KDSSLRLPNGARGKVVDVQVFDRQHDRDLPAGVETMVRVSVAQRRKISEGDKMAGRHG
                     NKGVISKIVPIEDMPYLDDGRSIDIILSPLGVPGRMNIGQVMETHLGWAAERIGFRAI
                     TPVFDGATEQEIATELGRAWLVDHAWEVASDWAWDWLAEINYDASTLENEDQARRMFI
                     VAWLSDLGYDAARIEVDEPYARRSVAREWLRSRDWNPDEVFPLNPEDLPKTRWLEIDP
                     LAVEVCVREWYAYVLERQDALLPESVKVDPTKTELDKLIELSNEITTQTHIPLPTLGK
                     ERLFDGKTGRPFDQPVTVGVLHMLKLAHLVEDKAHARSTGPYSLVTQQPLGGKAQFGG
                     QRFGEMEVWALEAYGAAHTLQEMLTVKSDDTQGRVKTYESIVKNEPIEEPGVPASFRV
                     LVKELQSLGLAVEAVTEGGEVVRFGKEDDKRTSRKWAGDDFMALARANR"
     gene            4511..4942
                     /gene="rpsL"
                     /locus_tag="PLC06_09880"
     CDS             4511..4942
                     /gene="rpsL"
                     /locus_tag="PLC06_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRO90796.1"
                     /translation="MPTINQLVRKGRSSKSKKSKSPALQYTLNSFKQRRTRQPMGAPQ
                     KRGVCTQVKTMTPKKPNSALRKVARVRLSNGIEVTAYIPGEGHSLQDHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDAGGVVNRKKGRSKYGTKVAKNVKKK"
     gene            4991..5461
                     /gene="rpsG"
                     /locus_tag="PLC06_09885"
     CDS             4991..5461
                     /gene="rpsG"
                     /locus_tag="PLC06_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRO90797.1"
                     /translation="MSRRIRPEKREIEPDLRYGNVHVSMFVNRLMYDGKKSVAQSLIY
                     DSFDMIEERVGRPGVEVFEQALQNVSPQVEVRPRRVGGATYQVPVPVDSYRQLSLAMR
                     WLLTAARNRGGQTMADKLANEFVDASNNQGAAVKKRDDTHRMAEANRAFSHFRA"
     gene            5469..>6877
                     /gene="fusA"
                     /locus_tag="PLC06_09890"
     CDS             5469..>6877
                     /gene="fusA"
                     /locus_tag="PLC06_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRO90798.1"
                     /translation="MADFPLERIRNIGIIAHIDAGKTTTTERILYYTGVIHRMGETHE
                     GTATMDSMDQERERGITISSAATTAFWLDHQINVIDTPGHIDFTAEVQRSLRVLDGGV
                     VVFDAVAGVEPQSETVWRQANGYDVPRICYVNKMDRVGADFKRTVGMIKNRLGANPIP
                     VQYPIGEESSFRGIVDLLTMQAYFWSDDDSGARPTAVAIPDDVRAEVDAAHHDMVERI
                     AETDDELTLRYLEGEEIEVEELRAALRRATVRNQATPVFCGSALRNRGIQRVLDGVVY
                     YLPSPLDIPAIKGDNPYTGKPEERHPSVDEPFSALVFKIVTDPYVGRLAYFRVYSGVV
                     QVGDSVLNSTKDKRERVGRILRMHADHREDLKEVRAGDIAATLGQKNTFTGDTLCDAK
                     APIILESIDFPEPVIQLAIEPKSNLDQDKMGNALRALSEEDPTFQVKVDDQTGQTVLF
                     GMGELHLEVLVDRLMREFKV"
BASE COUNT         1480 a         1833 c         2053 g         1511 t
ORIGIN      
        1 ataaactaca agttcgcgca agaccgcctc ttgatcgcga gctatagaat tgaccgtaca
       61 gtgaaagatc aatcgagcag ggtgcaagcg tgggttgcac cttttctgtg ttaggtggtt
      121 gacaagttat tccaaggcga tgtgttttcg cccgccaggg agcaggtgac tctatatgtc
      181 cagcatttat accaaaagct acgctcgtac aacgaaaccg atggaattgc cgacgttgat
      241 tgacgttcag ttacgctcgt ttgagcagtt catcagcgaa ggattggcgg agctttttga
      301 tgagatttct cccattgaaa gctttaacgg tgatttaaaa ctttatttcc ccagcaagcg
      361 caaggaagtt gcggatttca atctgaccta ctggttcggg gagccgaagt acaatgtcga
      421 ggagtgcgtg gagcgcgaca tgagctatgc cgctccgctc tatgtgcgcg ttcttctcta
      481 cagccaggat ttggaccaac cgattgagca ggatattttc atgggcgatt ttccgctcat
      541 gacctcggcc ggtaccttca ttattaatgg tacggagcgc gttgtcgtca gccagttgat
      601 ccgctcgccc ggcgcttatt ttgaggtcga ggaagagcgc gcgaccggcc gcccgctggc
      661 gatggggaaa ttaatccctg atcgcggcgc gtggatggag tttgaaacgc gcaagaccga
      721 ctacattacc atcaagttta atcgcaagcg cacggtgccg gtcactctgt tcctgcgtgc
      781 catggccgcg atccccgacg gcctgggcaa gccgctgatc agcgagggca ccgatgaaga
      841 gttgctggcc ttgtttgccg acatcgacac aaacggcgag cacctctaca ttcagggcag
      901 tatcgagcag gaaccgcatt gggaccagga aaagtctatt ccccaacagg cgctgatcga
      961 gttctacaag cgtatgcggc cgggcgaccc gcccacactc gacaacgcct cccaatatct
     1021 ccaggagcag ctgtatgaca gccgccgcta tgacctggcg gccgttggtc gttacaagct
     1081 gaacaagcgc cttaacctgc agaacgaagt cccgctcgat catcgaacga tcacccagca
     1141 ggatatcgtc aacttcgtcc gccacatgat cctcatcaat aatggccagg aagggccgga
     1201 tgacatcgat catcttggca atcgccgcgt gaagactgtt ggcgaattga ttcaggcgaa
     1261 gttgcgtgtc ggcctgcggc gcatggagcg cgtggttcgc gagcgcatgt caatccgtga
     1321 gagtgacagc gtcacccctg tggcattgat caacatccgc ccggtggtgg ctgccatccg
     1381 cgaattcttc ggcagctccc agttgagcca gttcatggag caggccaacc cgttggccga
     1441 gctcacccac aagcgcacct tgtcggctct aggccctggc ggtctgcgtc gtgagcgcgc
     1501 gggatttgaa gtccgtgacg ttcatcatag ccactacggc cgcatctgtc ctattgagac
     1561 gccggaaggt ccgaacatcg gtcttatcgg tcgtctggcg acctatggcc aggtcaataa
     1621 atatggattc attgaaacgc cctatcgccg ggtgatgaac aagttgccgg tgggtgatgc
     1681 gctgataaac catgatgtgt ttgtcaatgt gactgatcct tcgacgggcg agatcatcat
     1741 tgcccaggga gagaccgtta cggccgagca tctgaatgcc ttgcgcgaag cgggcattca
     1801 ggaagtgcag gtgcggccct tcctgactga ggaagtcctc tatatgtcgg ccgatgagga
     1861 agatcgttac acgattgccc aggccaacgc ccagatcgat gatcgcggtc agtttgtcga
     1921 tgcgcgcgtt tcttcgcgcc gcaatcagca attccgtttc acttccgtcc agcgaattga
     1981 cttcatggac gtcgccccgc gtcagatcgt cggcgtgtcg gccgcgttga tcccgttcct
     2041 ggagcatgac gacgccaatc gtgccctcat gggctccaac atgcagcgcc aggccgttcc
     2101 gctgctccag ccggatgtac cgctggtcag cacgggcatg gaattgcagg cggccctgaa
     2161 ctcaggtcag gtcgttctgg ccgatcagat cggcgaagtc gttagcgttt ctggcgacaa
     2221 gatcgtcgtc atgggcgaca acgggccgcg catctataac ctgcgcaagt acaaccgctc
     2281 caaccagagc acgtgcatcg accaacaccc tgttgtgcaa cgcggtcagc gcgtgcaaat
     2341 tggcgatgtg ctggccgata gctcttcgac gcattacggc gagctggctc tgggtcagga
     2401 cgttgtggta gctttcctct catgggaggg tggcaatttc gaggatgcta tcctcgtgag
     2461 cgaggcgatg gtccgacggg atcgtttcac ctccattcac attgaaaagc atgaggtcga
     2521 ggcgcgcgat accaagctgg ggccggagga gattacctac gatatcccca acgtgagcga
     2581 agacgcgctg aaggatctcg atgagcgcgg catcattcgc gtcggtgcgg atgtcaatga
     2641 gaatgatatt ctggtcggca agatcacgcc caagggcgag aaagaactaa gcccggaaga
     2701 aaaactgctg cgcgccattt ttggtgacaa ggctcgcgag gtcaaggact ccagcttgcg
     2761 gctgcccaac ggcgcacgcg gcaaggtcgt cgatgtgcag gtgttcgatc gccagcacga
     2821 ccgcgacctt cccgccggcg tcgagacaat ggttcgtgtc agtgtggccc agcgccgcaa
     2881 gatttccgag ggtgacaaga tggccggccg tcatggcaat aagggcgtca tctcaaagat
     2941 cgtccctatc gaggatatgc cctatctgga tgatggtcgc tcgatcgaca ttatccttag
     3001 cccgttgggc gtgcctggac gtatgaatat tggtcaggtc atggaaaccc acctgggttg
     3061 ggcggccgag cgaatcgggt tccgggcaat cacccctgtg ttcgacgggg ctaccgaaca
     3121 ggagatcgcc actgagttgg ggcgtgcctg gctggtcgat catgcctggg aggttgccag
     3181 cgactgggcc tgggattggc tggccgagat caactacgac gcatcgacgt tggaaaatga
     3241 agatcaggct cggcgtatgt tcattgtggc ctggttgagc gatttgggtt acgacgccgc
     3301 ccggattgag gtggatgaac cctatgcccg gcgctctgta gcccgcgaat ggttacgcag
     3361 tcgtgattgg aaccccgacg aggtattccc gcttaaccct gaagatctgc ccaagactcg
     3421 ctggttggag atagatcccc ttgcggtcga ggtatgcgtc cgtgaatggt atgcatacgt
     3481 tctggagcga caggatgccc tcctgcccga atccgtgaag gtcgatccga caaagacgga
     3541 actggataag ttgatcgagc tttcgaacga gatcacgacc cagacgcaca ttccgttgcc
     3601 gacattgggc aaggagcgtt tgttcgacgg taaaaccggg cggcctttcg atcaaccggt
     3661 gaccgtcggc gtgctccaca tgctgaagct ggctcacttg gtcgaagata aggcgcatgc
     3721 ccgttcgact ggcccgtact cactggtgac gcaacagccg ttgggcggca aggcccaatt
     3781 cggtggtcag cgcttcggcg agatggaagt ctgggcgctg gaggcttatg gcgcggccca
     3841 caccctgcag gagatgttga ccgtcaagtc ggatgacacc cagggacgag tcaagaccta
     3901 cgagtctatc gtcaagaatg agcctatcga agaacctggc gttccggcat cgttccgcgt
     3961 gctggtgaag gagcttcaga gcctgggcct ggccgtggag gctgtgaccg agggtggtga
     4021 ggtcgtgcgg tttggcaagg aagacgacaa gcgaaccagc cgcaagtggg ccggtgatga
     4081 tttcatggcc ctggcccgcg ctaatcgcta acggtattat cgccgatcag gcgaagttat
     4141 ccgataaaac gaacgttgtg agtcttgaca tctattgtga atctgataag attgacgatc
     4201 gtatgcctag ccgagcagga tagcgacgtt ttgagtcgtt attaatcgaa taggtttgaa
     4261 acgatggagg ccatcgcgac attttaggga cgatggcctc ctattgtcgg gtctggtagg
     4321 gtgtttctgt ccccttcctc catctgatta ttgatttctt gggttattca caaacgaata
     4381 gaagacctgc cactactgct ggtgataagg aacgaaaatt cgtccccctt ccgaggcagc
     4441 agtttgtagt gttttctgtc tgaatgtcgg tatcgacacg gacagattta gataaggaga
     4501 aatgtaagcc ttgcctacta ttaatcaact tgtccgcaaa ggacgtagtt ccaagtcgaa
     4561 gaagagcaag tctccggcat tgcaatatac cttgaattct ttcaagcaac ggcgtactcg
     4621 ccagcctatg ggtgcgcccc aaaagcgggg tgtttgcacc caggttaaga cgatgacgcc
     4681 gaagaagccg aattcggcgc tgcggaaggt ggcacgtgtt cgcttgtcga acggcatcga
     4741 agtgaccgcc tatattcctg gagagggtca ctctttgcag gaccactctg tcgtgttggt
     4801 gcgtggtggc cgtgtcaagg acctgcccgg tgtgcgttat cacatcgtgc gtggcactct
     4861 ggacgccggc ggcgttgtca atcgcaagaa gggccgttcc aaatacggca ccaaggtcgc
     4921 caagaacgtc aagaagaagt aattggaaga tttgggtggt cgatgaagga ccgccgggga
     4981 atgagaagtt atgtctagac gaattcgtcc ggaaaaacga gaaattgaac ccgacctgcg
     5041 ttacggcaac gtgcatgtgt cgatgttcgt taatcgcctc atgtatgatg gcaaaaagag
     5101 cgtggctcaa agcctgattt atgacagctt cgacatgatc gaagagcgtg ttggccgtcc
     5161 gggtgtcgag gtcttcgagc aggcgctgca aaatgtgtct ccacaagtcg aagtgcggcc
     5221 gcggcgtgtt ggtggtgcta cctaccaggt ccctgttccg gtggattcct accggcagtt
     5281 gtcgctggcc atgcgctggc tgctgacggc agctcgcaat cgtggcggcc agacgatggc
     5341 cgacaaactg gctaatgaat ttgttgatgc atccaataac cagggtgcgg cggttaagaa
     5401 gcgtgacgac acgcaccgca tggcggaggc caaccgcgcc ttctcccatt tccgggccta
     5461 agagagtcat ggctgatttt ccgttagagc gaatccgaaa tatcggcatc attgcccata
     5521 ttgacgccgg taagacgacg accaccgagc gcatcttgta ttacaccggt gtaatccatc
     5581 gtatgggcga gacccatgag ggcaccgcga cgatggactc catggatcag gagcgcgagc
     5641 gtgggatcac tatctcgtcg gctgctacca cagcgttttg gcttgatcac cagattaacg
     5701 tcatcgacac ccccgggcat atcgacttca cggccgaagt tcagcgcagt ttgcgcgtgc
     5761 tggacggtgg tgtggttgtt ttcgatgctg tggccggggt ggaaccacaa tccgagacag
     5821 tttggcgaca agccaacggc tatgatgttc cgcgaatttg ttacgttaac aagatggatc
     5881 gcgtcggtgc cgacttcaag cgcaccgtcg gcatgatcaa gaatcgtttg ggagccaacc
     5941 cgattcctgt tcaatacccc attggcgaag agtccagttt ccgcggcatt gtcgacctgc
     6001 tgaccatgca ggcttatttc tggagtgacg acgattccgg cgcgcgcccc acggctgtgg
     6061 cgatccccga cgatgttcgg gctgaggtag acgccgccca tcacgatatg gtggaacgaa
     6121 tcgccgagac cgatgatgaa ctcaccttgc gctacctgga gggtgaggag atcgaagttg
     6181 aggagctacg cgccgccttg cgtcgggcga cggtccggaa ccaggcgacg ccggtgttct
     6241 gtggcagcgc ccttcgcaat cgtggtattc agcgcgtgct cgacggcgtt gtttactatc
     6301 tgccctcgcc gctggacatc ccggctatca agggtgacaa cccctatacc ggaaagccgg
     6361 aagagcgcca tccttcagtc gatgaacctt tttcagctct ggtcttcaag attgtcacgg
     6421 atccctatgt gggtcgtctg gcctacttcc gcgtctattc cggcgtggtt caggtgggcg
     6481 actcggtcct gaacagcacc aaggacaagc gcgagcgcgt cggtcgcatc cttcgcatgc
     6541 atgccgacca tcgcgaggac ttgaaagaag tccgcgcggg cgacattgcg gcgacgctgg
     6601 gccagaagaa taccttcacc ggcgacacgt tgtgcgacgc gaaagctccg attattctgg
     6661 agtcgatcga tttccccgag cctgtcatcc aactggcgat tgagccgaag agcaatctcg
     6721 atcaggacaa gatgggcaac gccttgcgcg ctttgtccga ggaagacccg acattccagg
     6781 tgaaggtcga tgatcagacg ggacagacag tcctcttcgg tatgggtgaa ttgcatcttg
     6841 aggttctcgt ggatcgcttg atgcgcgagt tcaaagt
//
LOCUS       DAOMQG010000165         6865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4740_length_6865_cov_5.965932, whole genome shotgun sequence.
ACCESSION   DAOMQG010000165 DAOMQG010000000
VERSION     DAOMQG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_6865_cov_5.965932"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1086
                     /locus_tag="PLC06_09895"
     CDS             196..1086
                     /locus_tag="PLC06_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRO90799.1"
                     /translation="MTDREDRRALRWMLLPYGAGIVVLFVIPALMSFALAFFRYDTLN
                     PPRFAGTLNFTLVGTDSLFSLSVANSLALVVLPAPLRVFGAFLAARLARGGRLRHLLR
                     AAVFLPSAVPASAFALAWLWILNPLYGPLNRALMSLSLGAPGWLSDAQWARPGVALAL
                     LWLVGEGFLVCLAALYDLPPDLEDAAAADGAGAWATMRHVLLPLMAPVLLLLMARDAV
                     LLLQESFTLAWLLTGGGPYYATFTLPQFIYEAAFGLLDFGAASAALWALYAVTGLLLA
                     LAYGVARQWQVGLSEEEFLL"
     gene            1090..1941
                     /locus_tag="PLC06_09900"
     CDS             1090..1941
                     /locus_tag="PLC06_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRO90800.1"
                     /translation="MKPPAASAGLIVLRLLVAALFVLPLLFVILAAFRPPGAPLAGPL
                     WPASPTLDNFRRLFAVLPIGRFTMNSLRVVLVAVPLTVLVASWAGYAMARLPRPTQRR
                     WVLASFLVLMVPGVALWSSRFILYSRLGIGGSPAVLVIPALMGTSPFFVLMFYRAFRR
                     IPTAIYDAARLDGATTWQTWGGVALPMARPTALAVAALAFVLYWGDFVSPLIYLGDAG
                     SYTLPVGLQLLEQLGRSNYPLLMAGALWSMAVPLTLVVVGVGAVAFAERARRTDKLQK
                     LDKEGYR"
     gene            1938..3221
                     /locus_tag="PLC06_09905"
     CDS             1938..3221
                     /locus_tag="PLC06_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRO90801.1"
                     /translation="MNYLRRMIPLFCLFVLLIGCRPAEPAAVSFMVFGDSAEFAAYES
                     LVAAFEAGHADIDITLSHIPSQGDYRKKLATDFAAGSPPDVSLLNYRRVPPFIAAGQM
                     EPLGPYLDRSKIISRDDFYPITLEAFMWRGELTCIPQNISSLVVYYNRDLFKAAGVAE
                     PADDWTWDDFLAAAQALTLDSNGDGTTDQYGLGIEPSLLRLSPFVWQNGGRIVDDPAF
                     PTQLGLTRLPSLVALQWFVDLQVVHHVAPDRTAEASIDSETRFIEGTTAMYLNSRRGT
                     PTYREQAKFDWDIAPLPRGEQAAGVLHSDAYCLASAAKNKDAAWRFIEYANSPEGQAL
                     VARSGRTVPSLIAVAESDAFLDPAVRPARSRVFLDTIPALQSVPLLSTWEEIESTASE
                     EIERAFYGDITAEEAAILARDRTEEYFSLAVRPEQ"
     gene            3224..3400
                     /locus_tag="PLC06_09910"
     CDS             3224..3400
                     /locus_tag="PLC06_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90802.1"
                     /translation="MRGSSNRRNSFEHQLAELNAVRVDPSTPGAAETLRLALADRSNL
                     LVARDDLPPAYSTT"
     gene            complement(3385..3813)
                     /locus_tag="PLC06_09915"
     CDS             complement(3385..3813)
                     /locus_tag="PLC06_09915"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRO90803.1"
                     /translation="MPLTRLSSHLPVLFVLLLVSLAAAACGGKDGAEPENVYIADEAD
                     NGQTVTMKIGDMLQLMLAENPTTGYTWAIVTNDGDVLAPSGEPAYEVESDAIGAGGTR
                     TFMFQAAAPGTSVLQLVNARQWETAVEPAETFVMTVQVVE"
     gene            complement(3864..6806)
                     /locus_tag="PLC06_09920"
     CDS             complement(3864..6806)
                     /locus_tag="PLC06_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRO90804.1"
                     /translation="MKATKLAEFMAVCRARVAGELRDDRYSRMFYSTDAGLYQIFPHA
                     VLIPRTAEDVIAAVTIAAEFGVPVLARGAGTSLAGQAVNEALVIDFSRHLDQLVAVNA
                     GERWARVQPGMVVDDLNAALRPLGLQFGPDPASADRSTIGGAVANNATGSHSLLYGMT
                     ADHVRAMEVVLAGGRVARLGPDTLTDALRMHDLRVADDLGRLVADPAHQQTIRADTPH
                     YWRRCGGYNLDRLLPDASQNLAQLICGSEGTLAVMTEITLNLVPRPPHKVLGLVHFDD
                     LHVALEAVAAILETDPAAVELLDTLSLTLCRGVPAYARMLASVIDGRPLCLLVVEYQG
                     ESAAEALDGLARLERRLARAGAGHTGLTQATTAEAMAAVWGVRKAGLGLLMSMKGDMK
                     PVPFIEDAAVPVESLPDYVRRVEEFCAGLDTPITYYAHAGAGCLHIRPLVNRRSAAQV
                     AHIPDIARFAAELVRGYGGALSSEHGDGRTRSWLNEAFYGPALYDLFRQVKRIFDPDN
                     LLNPGIIVDAGPMLDNWRALPNSPGPSRTDFADYEVADPIPLRVAVKTATSSTPADGF
                     VHAVEMCNGAGVCRRQTGTMCPSFMVTREEEHSTRGRANALRAAMSGVLPASELTSPR
                     MARVMDLCISCKACKAECPSAVDMARIKTEFLARYYEVHGVGLRPRFFGHAAALNRLG
                     SGWRAPLANAVLRSRPGRAAMARLLRLAPQRPLPEMARVPFEAWWRRHHAPSIAQPIA
                     TIVLLIDPFTNANHPEVGIAAVEFFEAIGVEIVAAPALDDGRPCLSKGLVAGARRAAE
                     RTVAALAPFVERGLPVVGLEPSSLLTLRDEYLHLLPDDGRARALADHALTFEEFVDGL
                     AEQRDLRPFFIGEPREVLLHGHCHQKALVGTGPARRVLALPGYRVTEVDSGCCGMAGS
                     FGYEDEHYELSMQMGERRLLPAVRETPPETIIVTAGMSCREQIAHGSGRAALHPAQVL
                     RGALRSGR"
BASE COUNT         1049 a         2392 c         2208 g         1216 t
ORIGIN      
        1 gcaaggaagg cggctttctg ggctttggcg gcgtgcgcgt ctccgacgcc gagcagacga
       61 tgctggcgca gattcgggca gaactgggct gattactgat ctactgaata ctgatctact
      121 gaatactgat ctaccgaata ctgatctact gaatactgat ctactgaata ctgatctgac
      181 cgcccaccca acgacatgac cgaccgggaa gaccgacgcg cgttgcgctg gatgttattg
      241 ccctatgggg cgggcatcgt cgtgctgttc gtcatcccgg cgctgatgtc gttcgcgctg
      301 gcgttcttcc gctacgacac gctgaacccg ccccgtttcg ccggaacgct caattttacg
      361 ctggtgggca ccgactcgct gttcagcctg agcgtcgcca attcgctggc cctggtggta
      421 ttgcccgcgc cgctgcgcgt gtttggcgcg tttctggcgg cgcggctggc gcgcggcggc
      481 cggttgcgcc atctgttgcg ggcggccgtc tttctgccgt cggccgtgcc cgcctcggcg
      541 ttcgccctgg cctggctgtg gattctgaac ccgctttatg gcccgctgaa ccgcgccctg
      601 atgtcgctgt ctttgggcgc gcccggttgg ctgtccgacg cgcaatgggc gcggccgggg
      661 gtcgctctgg cgctgctctg gctggtgggc gaggggttcc tcgtctgttt ggcggcgctc
      721 tatgacctgc cgcccgatct ggaagatgcc gcggcggccg atggcgcggg agcatgggcc
      781 acgatgcgcc atgttctgct gcccctgatg gcccccgtcc tgctgctgct catggcccgc
      841 gacgccgtgc tgctgttgca ggaatcgttc acgctggcct ggctcctcac cggcggtggg
      901 ccgtattacg ccaccttcac cctgccgcaa ttcatctacg aagccgcgtt cggtctcctc
      961 gatttcggag cggcatcggc tgcgctgtgg gcgctctacg ccgtcaccgg gctgctgctg
     1021 gcgctggcct atggcgtcgc tcgccaatgg caagtcggcc tgagcgaaga ggagtttctg
     1081 ctctagccga tgaaaccgcc agccgcttcc gccggtctca tcgtcctgcg cctgctggtg
     1141 gcggcgctgt ttgttctgcc gctgctcttc gtgatcctgg ccgccttccg cccgcccggc
     1201 gcgccgctgg ccgggccgct ctggccggcg tcgcccacgc tcgacaactt ccgccgtctg
     1261 tttgccgtct tgcccatcgg ccgcttcacg atgaactcgc tgcgggtcgt ccttgtcgct
     1321 gtgccactca cggtgctggt cgcctcgtgg gcgggctatg ccatggcccg gctgccgcgg
     1381 ccaacccagc gccgctgggt gctggcttcg ttcctcgtgc tcatggttcc cggcgttgcc
     1441 ctctggtcat cgcgcttcat cctctacagc cgcctcggca tcggcggatc gccggcggtg
     1501 ctggttatcc cggccctcat gggcaccagc ccgttcttcg tgctgatgtt ctatcgcgcg
     1561 ttccggcgca tcccgacagc gatctacgac gcggcgcggc tggacggcgc gacgacgtgg
     1621 cagacctggg gcggggttgc tctgcccatg gcccggccaa cggccctcgc cgtggccgcg
     1681 ctggccttcg tcctctattg gggcgatttc gtctcgcctc ttatttactt gggggatgcc
     1741 ggaagctata ctttacccgt cgggctgcag ttgctggagc agctcggccg ttccaattac
     1801 ccgctgttga tggccggggc gttgtggtcc atggcggtcc cgttgaccct ggtggtcgtc
     1861 ggggtgggag ccgtggcgtt tgccgaacgg gcgcggcgaa cagataaatt gcagaaattg
     1921 gacaaggaag gataccgatg aattatctcc gacgcatgat cccgttgttc tgtttgttcg
     1981 ttctcctgat cggctgtcgc cccgcggaac cggcggccgt ctcctttatg gtctttggtg
     2041 attcggccga attcgccgcc tatgagtcgc tcgtggccgc gtttgaagcc ggccacgccg
     2101 atatcgacat caccctgagc cacattccgt cacagggcga ctatcgcaaa aaactggcga
     2161 ccgatttcgc ggcaggctcg cctcccgacg tgagcctgct caactaccgg cgcgtgcccc
     2221 cgttcatcgc cgccggtcag atggagccgc tcggccccta tctcgaccgc agcaagatca
     2281 tcagtcgcga tgatttctac cccatcacgc ttgaagcgtt catgtggcgc ggcgaactca
     2341 cctgcatccc gcagaatatc tccagcctcg tcgtctacta caaccgcgac ctgttcaagg
     2401 ctgcgggcgt ggccgaaccg gccgatgact ggacatggga tgattttctg gctgccgccc
     2461 aggcgctgac cctcgacagc aacggcgacg gcaccaccga ccagtacggt ctcggcatcg
     2521 agccgtcgct gctgcggttg tcccccttcg tctggcaaaa cggcggccgc atcgtcgatg
     2581 acccggcctt ccccactcaa ctcggcctga cgcgtctccc gtcgctggtc gccctgcaat
     2641 ggttcgtcga tctgcaggtc gtccaccacg tcgcccccga ccggacggcc gaggcgtcga
     2701 tcgacagcga gacgcggttc atcgagggca cgacggcgat gtatctcaac agccgccgcg
     2761 gcacgccgac ctaccgtgag caggcgaaat tcgattggga catcgcccca ttgccgcgcg
     2821 gcgagcaggc ggcgggggtg ctccacagcg acgcctactg cctggcttcg gcggccaaga
     2881 acaaggatgc cgcctggcgt ttcatcgaat acgccaactc gcccgaaggg caagcccttg
     2941 tcgcccgcag cggccgcacc gtgccgtcgc tgatagccgt ggccgagtcc gacgccttcc
     3001 tcgaccccgc cgtccgcccg gcccgcagcc gcgtcttcct cgacactatc cccgcgctgc
     3061 agagcgtccc gctgctcagc acgtgggagg agatcgagtc cacggcgtcg gaagagatcg
     3121 agcgcgcgtt ctatggtgac atcaccgccg aggaggcagc aatactggcc cgcgaccgaa
     3181 cggaggaata tttctcgctg gccgtgaggc ccgagcaata gccgtgcgcg gcagttccaa
     3241 ccggcgcaac agctttgagc accaattggc ggagcttaat gccgttcgcg tcgacccatc
     3301 cacgccgggc gcggccgaaa cgctgcgcct ggcgcttgcc gaccgttcca atctgctggt
     3361 ggcccgcgac gatctgcctc ctgcctattc cacaacctga acagtcatca cgaacgtctc
     3421 cgccggctcc acggccgtct cccactgccg ggcgttgacc agttgcagca cgctcgtgcc
     3481 gggcgcggcg gcctggaaca tgaacgtcct cgtgccgccc gcgccgatgg cgtcgctttc
     3541 gacttcgtag gccggctcgc cgctgggggc cagcacgtcg ccgtcgttgg tcacaatcgc
     3601 ccatgtgtag ccggtcgtcg ggttctcggc cagcatgagc tgcagcatgt cgccgatctt
     3661 catcgtcacc gtctgcccgt tgtcggcctc gtcggcgatg tagacgttct ccggctccgc
     3721 gccgtccttg ccgccgcagg cagcggccgc aagcgacacc agcagcaaga cgaacagaac
     3781 aggaagatgg ctggacaagc gagtcagggg catggaacat cactccaatg atgaaataag
     3841 tcaatcgggg gatgataacc caactaccgg ccgctccgca atgccccccg cagcacctgc
     3901 gccggatgca gcgccgcccg gccgctgccg tgggcgatct gctcccggca gctcatcccg
     3961 gcggtgacga tgatcgtctc cggcggcgtc tcgcgcacgg cgggcagcag gcggcgctcg
     4021 cccatctgca tcgacagctc gtaatgctcg tcctcatagc cgaacgaacc ggccatgccg
     4081 cagcagccgg aatcgacctc ggtcacccgg tagccgggca gcgccagcac ccgccgtgcc
     4141 gggccggtgc ccaccagcgc cttttgatgg caatggccat ggagcagaac ctctctcggc
     4201 tccccgatga aaaacggccg cagatcgcgt tgctcggcca gcccgtcgac gaactcctcg
     4261 aaggtgagcg cgtgatcggc cagcgcccgt gcccggccgt cgtccggcag caggtggagg
     4321 tactcatcgc gcaaggtcag caggctgctc ggctccagac cgaccacggg caggccacgc
     4381 tcgacgaacg gggccagcgc ggccacggtg cgttcggccg cgcggcgggc gccggccacc
     4441 agccccttcg acaggcaggg gcggccgtca tcgagcgccg gagcagcgac gatctccacc
     4501 ccaatcgcct cgaaaaactc caccgcggcg atgcccactt cgggatggtt ggcgttggtg
     4561 aacgggtcga tgagcaggac gatggtcgcg attggttgtg cgatggaggg cgcgtggtgt
     4621 cgtcgccacc acgcctcgaa cggcacgcgg gccatctccg gcaaggggcg ctgcggggcc
     4681 agccgcaaca ggcgcgccat cgccgcacgg ccggggcgcg agcgcaggac ggcgttggcc
     4741 agcggcgcgc gccagccgct gcccagtcga ttgagcgccg ccgcatggcc gaagaaccgc
     4801 gggcgaagcc ccacgccatg gacctcgtag tagcgggcca ggaactccgt cttgatgcgc
     4861 gccatgtcga cggccgacgg acactccgcc ttgcacgcct tgcaggagat gcacagatcc
     4921 atcaccctgg ccatgcgcgg cgaggtcaat tccgacgccg gcagcacgcc cgacatggcc
     4981 gcccgcaggg cgttggcccg gccgcgcgtg ctgtgttctt cttcgcgcgt caccatgaag
     5041 ctggggcaca tcgtccctgt ctggcggcgg cacacgcccg ccccgttgca catctccacg
     5101 gcatggacga agccgtcagc cggggtggat gaagtggcgg ttttcactgc tacacgaagc
     5161 gggatgggat cagccacttc gtaatcggcg aagtcggtgc gcgacgggcc gggcgagttg
     5221 ggcagggcgc gccagttgtc gagcatcggc ccggcgtcga cgatgatgcc cggattcagc
     5281 agattgtcgg ggtcgaagat tcgcttcacc tgccggaaca ggtcgtagag agccgggccg
     5341 tagaacgctt cgttcagcca gctgcgggtg cggccgtcgc cgtgctcgct cgacagcgcg
     5401 ccgccatatc cccgcaccag ttcggcggcg aaccgggcaa tatcggggat gtgggccacc
     5461 tgcgccgcgc tgcggcggtt gaccagcggc cgaatgtgga gacagcccgc cccggcgtga
     5521 gcatagtagg tgatgggcgt gtccagcccc gcgcagaact cctcaacgcg gcgcacgtag
     5581 tcgggcaggg attccaccgg cacggccgcg tcctcgatga acggcaccgg cttcatgtcc
     5641 cccttcatcg acatcagcag ccccagcccc gccttgcgca ccccccacac ggccgccatc
     5701 gcctcggccg tcgtcgcctg cgtcaggccg gtgtggcccg cccctgctcg cgccagacgc
     5761 cgctccagcc gcgccaggcc atccagcgcc tcggccgcgc tctccccctg atactccacg
     5821 accagcaggc agaggggccg cccgtcgatc accgaggcca gcatccgcgc ataggcgggc
     5881 acgccgcggc agagggtcag gctgagcgtg tcgagcagct ccacggccgc cggatcggtc
     5941 tccagaatag ccgccaccgc ctccagcgcc acgtggagat cgtcgaaatg aaccagcccc
     6001 agcaccttgt ggggcggccg cggcaccaga ttgagcgtga tttcggtcat caccgccagc
     6061 gtaccctccg agccgcaaat cagctgggcc aggttttggc tcgcatccgg cagcaagcgg
     6121 tcgaggttat acccgccgca gcgtcgccaa taatggggcg tgtccgcgcg aatcgtctgt
     6181 tgatgagcgg gatcggcgac cagtcgtccc aaatcatctg ctacacgaag gtcatgcatc
     6241 cggagcgcat cggtcaatgt gtcggggccg agccgggcga cgcggccgcc ggccagcacg
     6301 acctccatcg cccgaacatg atcggcggtc atcccgtaga gcagggagtg gctgccggtg
     6361 gcgttattgg ccaccgcgcc gccgatagtc gagcggtcgg cgctggccgg gtcggggccg
     6421 aattgcagcc cgagcggccg cagcgccgcg ttgaggtcat caaccaccat ccccggctgc
     6481 acccgcgccc aacgctcccc ggcgttgacc gcgacgagct gatccaggtg gcggctgaag
     6541 tcgatgacca gcgcctcgtt caccgcctgg ccggccagac tggtgcccgc gccgcgagcc
     6601 agcaccggca cgccgaactc ggccgcgatg gtcacggcgg cgatcacgtc ctcggccgtg
     6661 cggggaatga gcacggcatg ggggaatatt tgatacaggc cggcgtcggt gctgtagaac
     6721 atgcgcgaat agcggtcgtc gcgcagctcc cccgccaccc gcgcccggca aacggccata
     6781 aactcggcca gcttcgtcgc tttcatccgg gtatagtagt ccgatgacgc gggttgtcaa
     6841 ccgggatact atttatcaat aaaat
//
LOCUS       DAOMQG010000166         6758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4832_length_6758_cov_12.229748, whole genome shotgun sequence.
ACCESSION   DAOMQG010000166 DAOMQG010000000
VERSION     DAOMQG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4832_length_6758_cov_12.229748"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PLC06_09925"
     CDS             complement(<1..1364)
                     /locus_tag="PLC06_09925"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRO90805.1"
                     /translation="MRHAFKRLSPVPSDLDIAQAAVPRPISEIAAEIGLTDDDLIHYG
                     KYKAKVHLDTLPKLANRPTGKYVDVTAITPTPLGEGKTTTSIGLVQGLGALGHKAIAC
                     IRQPSMGPTFGIKGGAAGGGYSQVIPMEDFNLHLTGDIHAITAAHNLVAAAIDARYYH
                     EDRMNDERLGKVGLRRLNIDPYRTTWNRVTDTNDRALRKIVVGLGPEEEGRPRQTGYD
                     ITVASEIMAILALVNGDDYKGALKDLRRRAGRTVIGINKDGKPISLDDLEVAGAVTVI
                     MKDALHPNLLQTLEGQPAFVHAGPFANIAHGNSSILADRLALKLGDYVVTESGFGADI
                     GMEKFFNIKCRYSGLLPDCVVLVATIRALKMHGGGPRVVAGRPLDKAYTDENLPLLEA
                     GMANLAAHIRNAKRFGIPVVVAVNRFPTDTDAEVKAVVDGAIAAGAETAVMSNHWAEG
                     GDGA"
     gene            complement(1534..2082)
                     /locus_tag="PLC06_09930"
     CDS             complement(1534..2082)
                     /locus_tag="PLC06_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRO90806.1"
                     /translation="METSIIISGFGGQGVLFAGQLLAYAGMDAGKHVTWIPSYGPEMR
                     GGTAHCVVIISDDVIGAPIVTRPDITVVLNQPSFDKYEPLVKPGGLLVVNSSLISAES
                     YRDDIQVVYVAANAIAEEWGTAKMMNMAALGAMLAGRPVLSLDVVKKALVEHFPAGKQ
                     HLVEANLKVIEHGSLISQPSNS"
     gene            complement(2102..3418)
                     /locus_tag="PLC06_09935"
     CDS             complement(2102..3418)
                     /locus_tag="PLC06_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRO90807.1"
                     /translation="MQSMKGSAIRELLKLTERPDIIAFGGGMPAPEVFPVDAFRDAAE
                     RVLARQGKQALQYSATEGYRPLREWIARKMAAYGIESAPENVLITSGSQQALDLLGKV
                     LINPGDLILTERPTYLGALQAWRAYQAEFATVPMDNEGLQVDLLEEALTAGPKFMYIL
                     PNFHNPAGVTLSLERRRKLIEFSDRYGVPIIEDDPYGELRYEGEHLPPLVVLDSELFK
                     GRKGRDVESNGYYDISKPINGNGRAYMRGNVIYLSTFSKTLAPGLRLAWMVAPVEVIQ
                     RCNVAKQGMDLHTATLNQLIAHEILTGGEQGDDFLKGHVRLIREVYRERRDVMLTAME
                     RHFPDGVTWTHPDGGLFLWVTLPNYLDASEILKDAIEQKVAYVPGSAFHPGNDGKNTL
                     RLNFSYCNPEKINEGIKRLGHVFGQAIEQHGMMDMESLARSLPVAF"
     gene            complement(3499..4311)
                     /locus_tag="PLC06_09940"
     CDS             complement(3499..4311)
                     /locus_tag="PLC06_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRO90808.1"
                     /translation="MAVSPTDQKIMNAPAVVKEEIVYQRPVALTNVHTHYCPGCTHGT
                     AHRLVAEVIDELGIREDTILVASVGCSVFAYNYFEVDACEAAHGRAPAMATGVKRVHP
                     DKVVFTYQGDGDLASIGMGEIMHAAARGEKITTIFINNAVYGMTGGQMAPTTLPGQIT
                     TSSPFGRDLQLCGAPVRMVELLSNMDGTAYAVRRSLHDARHTIQARKAIRTAFEAQIK
                     GLGFSIVELLSSCPTNWGMTASESLDWIRDSMIPTYPLGDYKVAPELVQSRK"
     gene            complement(4292..5407)
                     /locus_tag="PLC06_09945"
     CDS             complement(4292..5407)
                     /locus_tag="PLC06_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRO90809.1"
                     /translation="MAKQLLKGNVAFAEAAIRAGCEVYFGYPITPQTELLEHMSSRMP
                     ELGRVFLQAESEVAAVNMVYGAAAAGARVMTSSSSPGISLMQEGFSYMAASNVPAVVI
                     DIMRGGPGLGNIQPSQSDYNQVTKSAGHGDFHPIVLAPSTVQEAIDLTILAFDLAEKY
                     RTLVFVIADGTIGQMMEPAELPEMRPVSTERPTWAVTGARDRSHNVITSLFLGAEQLE
                     QLNLDLQDKLLQIQQAGEVRYDEAYLEGAEVVIVAFGTSARVAQTAVKRMREAGLPVG
                     LFRPITLWPFPEKEIAELPKRIPALKAIQVVEMNAGQMVHDVREAIAGQVPVQFMGRM
                     GGIIPVPEEIEEKVRLTLTPAQMPATLKESSNGRVAH"
     gene            complement(5514..5723)
                     /locus_tag="PLC06_09950"
                     /pseudo
     CDS             complement(5514..5723)
                     /locus_tag="PLC06_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            6121..>6758
                     /locus_tag="PLC06_09955"
     CDS             6121..>6758
                     /locus_tag="PLC06_09955"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRO90810.1"
                     /translation="MEFGIPKEVRDLESRVGLTPAGVASLVRHGHSVYVEKNAGAGAG
                     FVDETYRLSGAQIVYSAAEVYGRSDVVVKVTRPTATEHALFQPGQTLIAFLHMAVASP
                     DFLEALREKEITAIACEMIRQPNGNHPILVPMSEVAGRLAPVIAGQLLMTNGGGRGTL
                     LSGIPGVPRGSVAIIGGGVLGANAARAFLNAGAQVVVLDNDIARLKHLDEAL"
BASE COUNT         1385 a         1934 c         1952 g         1487 t
ORIGIN      
        1 atggctccat cgccaccttc ggcccaatga ttgctcatga cggccgtttc tgctcccgcg
       61 gcgattgccc cgtctaccac tgccttgact tcagcgtccg tatccgttgg gaagcggtta
      121 acggcgacaa cgaccgggat gccgaagcgc ttggcattac gaatgtgagc cgccaggttg
      181 gccataccgg cctccagcag ggggaggttc tcatccgtat atgccttgtc caacggccgc
      241 cccgccacca cgcgcgggcc accaccatgc atcttcagag cgcgaatggt ggcgaccagc
      301 accacgcaat ccgggaggag gcccgaataa cggcacttga tgttgaagaa cttctccatg
      361 ccgatgtctg cgccaaagcc ggactcagtg acgacataat cgcccagctt cagggccagc
      421 cgatcggcca ggatggacga gttgccgtgg gcgatgttgg cgaatggccc ggcatggacg
      481 aaggcgggct ggccttccag cgtctgcaag agattgggat ggagtgcatc cttcatgatt
      541 accgttaccg cgccggcgac ttccagatca tcaagtgaaa ttggcttgcc gtctttgtta
      601 atgccgatca ccgtgcggcc tgcccggcgg cgcagatctt tcagagcgcc cttgtaatcg
      661 tcgccgttga cgagcgccag gatagccatg atctcgctgg caacagtgat gtcgtagccg
      721 gtttggcgcg gccgcccttc ttcctccggc cccaggccaa cgacgatctt ccgcaaggcg
      781 cgatcattgg tgtcggtcac ccggttccag gtagtgcgat agggatcgat attcaagcgt
      841 cgcagcccca ctttccccaa gcgctcatcg ttcatgcgat cttcgtggta gtagcgcgcg
      901 tcgatggctg ccgccaccag gttatgggcc gcggtgatgg cgtggatatc accggtcaga
      961 tgaagattga aatcctccat ggggatgacc tggctgtagc cgccacccgc cgcgccgccc
     1021 ttgataccga aagttgggcc catgctcggc tgccggatgc aggcaatggc cttgtggccg
     1081 agagcgccta atccttgtac tagaccgatt gaggtcgtcg tcttgccctc accgagggga
     1141 gtcggtgtga tggcggtgac atcaacgtac ttgccggtcg ggcggttggc caactttggc
     1201 aatgtatcga ggtgaacctt ggccttatac ttaccatagt ggattaagtc atcatccgtc
     1261 aggccgattt cggcggcgat ttcagatata ggacggggaa cggctgcctg ggcgatatca
     1321 agatcagatg ggacagggga taggcgcttg aatgcgtgtc tcattttacc tctgggtata
     1381 ttgccaccag cttttaggca gatggcggga aaagattggg aaattcattt tatcggaatc
     1441 gttgaacccg gccaaccaaa gacgggcaca ataattgccc ttcatttggg aatatgaagg
     1501 gcaatctcga ttacggttaa ttaaccacga tcatcaactg ttgctgggct ggctgatcag
     1561 cgaaccatgc tcgatgacct ttaggttggc ctcgaccaga tgctgtttgc cggccggaaa
     1621 atgctccact aatgccttct tgacgacatc gagagacaac accggccgac ccgcgagcat
     1681 cgcgcccagc gcggccatgt tcatcatctt ggccgttccc cactcttcgg caatggcgtt
     1741 ggccgctaca taaacgacct ggatgtcatc ccggtacgat tcggccgaga tgagcgaact
     1801 gttgaccacc agaagcccgc ctggcttgac tagtggctcg tacttgtcga aagatggctg
     1861 attaaggacg acggtgatat ccggccgcgt gacgattggc gcgccgatca catcatcact
     1921 gatgatgact acgcaatggg ccgttccgcc gcgcatctcc ggcccatatg agggaatcca
     1981 ggtgacgtgc ttgcccgcat ccatgcccgc ataggcgagc aattgcccgg caaacagaac
     2041 gccttgtcca ccgaatccag agataatgat tgatgtctcc atagggatat tccttttaag
     2101 gctagaaggc gacgggcaag gaccgagcaa gactctccat gtccatcatc ccatgttgct
     2161 cgatagcctg gccaaagacg tgcccgaggc gtttgatgcc ctcattaatt ttttccggat
     2221 tgcagtagga gaaattcagc cgcaatgtgt tcttgccgtc attgccgggg tggaatgctg
     2281 agccggggac gtaggcgacc ttttgctcga tagcatcttt caggatctcc gaggcgtcca
     2341 gatagttcgg caacgtgacc cacaggaaca ggccgccgtc cgggtgcgtc catgtgactc
     2401 catcggggaa atgtcgctcc atcgccgtca acatcacgtc ccggcgctcg cgatacactt
     2461 cgcgaatcag gcgcacgtga cccttcagga agtcatcgcc ttgctcgccg ccggtcagga
     2521 tctcgtgggc gatcaactga ttgagggttg cggtatgcaa atccatgccc tgcttggcaa
     2581 cgttgcagcg ctggatgacc tcaaccggcg cgaccatcca tgccagacgc agaccgggtg
     2641 ccaatgtctt ggagaaggtg ctcaggtaga tgacgttgcc gcgcatatag gcccgaccgt
     2701 tgccgtttat cggcttggag atgtcataat aaccgttaga ttccacgtca cggcctttcc
     2761 gacccttaaa cagttcagag tccagaacga ccaggggcgg caaatgctcc ccctcataac
     2821 gaagttcgcc gtaaggatca tcctcgatga tcggtacgcc atagcgatcg gagaattcaa
     2881 tcagtttgcg ccgccgttcg agggaaagag tgaccccggc cggattgtgg aagttcggca
     2941 ggatgtacat gaacttcggg ccggcagtta gcgcttcctc cagcagatcg acctgcaacc
     3001 cttcattatc catgggaaca gtcgcgaact ccgcctgata ggcgcgccag gcctggagcg
     3061 cccccaggta ggtgggcctt tccgtcagga tgagatcgcc gggattgatg aggactttgc
     3121 ccagcaagtc caatgcctgt tgcgagccgc tcgtgattaa cacgttctcg ggcgcggatt
     3181 cgatcccgta ggcggccatc ttcctggcga tccactctct cagcggccga tagccttcgg
     3241 tggcgctgta ttgcagggcc tgcttgcctt gcctggccag aacccgttcg gctgcgtccc
     3301 gaaacgcatc gacagggaat acttccggcg cgggcatacc gccgccaaac gcgatgatgt
     3361 ccggcctctc agtcagtttg agaagctcgc gaatcgccga gcctttcatt gattgcattc
     3421 gttgtgcgca gcgatgattc cagggagttg tcataggttt gctccttgcg cttttcgttc
     3481 taatgagatg aaatgatctt atttgcgtga ctgtactaac tcaggtgcga ctttgtaatc
     3541 gcccagcggg taagtaggga tcatgctgtc ccggatccag tccaacgatt ccgaagccgt
     3601 catgccccag ttcgttgggc aagacgacaa tagttcaacg atgctgaaac caagcccctt
     3661 gatttgggct tcgaaggcgg tacgaatggc cttcctggcc tgaatagtgt gccgtgcgtc
     3721 gtggaggctc cgccgaaccg cgtaggcggt accgtccatg tttgacaata gctccaccat
     3781 ccgaacaggt gcgccacaca gctgaagatc gcggccgaaa ggtgacgaag tcgtgatctg
     3841 gccgggaagt gtcgtcggcg ccatctgtcc acccgtcata ccatagacgg cattgttgat
     3901 gaagatggtg gtgattttct cgccacgagc ggcggcatgc atgatctcgc ccatgccaat
     3961 ggacgccaga tcgccatcac cctgataagt aaaaacaacc ttatcgggat gaacgcgctt
     4021 gacgccggtc gccatggccg gcgcgcggcc gtgcgcggct tcgcaggcat ccacctcaaa
     4081 atagttgtag gcaaatacgg aacagcctac cgatgcgacc aggatcgtat cttcgcgaat
     4141 ccccagttcg tcgataactt ccgccaccag ccgatgggct gtgccgtggg tacatcccgg
     4201 gcagtaatga gtgtggacat tggtcagcgc gacggggcgc tggtagacaa tctcctcttt
     4261 caccaccgcg ggggcgttca tgattttttg gtcagtgggc gacacggcca ttgctcgact
     4321 cctttagggt ggccggcatc tgtgccggtg taagcgtcaa ccgaactttc tcctcgattt
     4381 cttccggcac ggggatgatg ccgcccatgc gacccatgaa ctgaacgggg acttgtccgg
     4441 cgatcgcctc gcgcacatca tggaccattt gacccgcgtt catttccaca acctgaatgg
     4501 cctttagggc aggaattcgc tttggcagtt cggctatttc cttttcgggg aacggccaca
     4561 gggtgatggg ccggaaaagg cctaccggca aaccggcttc gcgcatgcgt ttgacggccg
     4621 tctgggccac gcgagccgat gtgccaaagg ccacgataac gacctcggct ccttccagat
     4681 aggcttcgtc atagcgaact tcgccggcct gctgaatttg cagaagcttg tcctgcaagt
     4741 ccaggttgag ctgttccaac tgttcagcgc ccaaaaagag tgaggttatc acgttgtgtg
     4801 accgatccct ggcgccggtg acggcccagg tcggtcgctc ggtagaaacg gggcgcattt
     4861 ccggcagttc ggccggttcc atcatctgcc cgatagtccc atcggcgatg acaaaaacaa
     4921 gcgtgcggta cttctccgcc aggtcaaatg ccagtatcgt gagatcgata gcttcctgaa
     4981 cggtcgaagg tgctaatacg atggggtgga aatcgccgtg gccggctgac ttcgtcactt
     5041 ggttgtaatc ggactggctg ggctggatgt tgcccaggcc ggggccacca cgcatgatat
     5101 cgatgaccac tgcgggaaca ttggaggcgg ccatgtagct gaagccttcc tgcatcagac
     5161 tgatccccgg gctactggag gacgtcatga cccgcgcgcc cgcggcggcc gcgccataga
     5221 ccatattcac cgcagccact tcgctctcgg cctggaggaa tacgcgcccc agttccggca
     5281 tccggctgga catatgctcc agtaattctg tttgtggggt aatggggtag ccgaaatata
     5341 cttcgcaccc ggcgcgaatg gcagcctcgg cgaaggcgac gttgcctttc aataattgct
     5401 tagccatggt gcagcacctc cgggattcca tgttcgacac tgtgtattgg tttcgtgggg
     5461 atgccctcca tggagcggcc cgcggctgtg cgtcctttct caatattgat gtcgcggtag
     5521 acagtgatgc agccgtcggg gcagaccgtg gcgcaaagcg cgcaaccggt acaatcatga
     5581 tgtgggtcta aaagaatcac gggcctgtat cctttgctgt tcagctcttc agccaaagcc
     5641 agaacatctt taggacaagc cccgagacac aactcacatc ctttgcaccg ttccacatca
     5701 attacaactc ttccagttgc catctaaagt acctccggtg agatgggaaa acgtgcctga
     5761 actcccctgg ctcttataaa acaagaaggg cggagcaatg ttaggcttgc aggctccgcc
     5821 ggattgcctt attcgatttt tacgtcgatc caagttgtca aggaacggga acgccaggca
     5881 agagtatcta tctagtgtca gtatcgcata cgactggctt aatctgtagt gacaaatggc
     5941 agttgaattt aatgaggaaa gtcacctgtt tgtagccggc cccatgctaa actaactttg
     6001 gctggcctgt acaggtttcg tgagctttat cggccaatgc atcgcagcat cacagccggc
     6061 cacttcccaa tttggttatt acgcctgcaa gattacgaat tgccggcagg aggtcgcatc
     6121 gtggaatttg gcataccaaa ggaggtgcgg gatctggaat cccgtgtggg cttgacccct
     6181 gcgggcgtgg catcgctggt gcgtcacggc cactccgttt acgtcgagaa aaacgccgga
     6241 gccggagccg gatttgttga tgagacctac cgccttagcg gtgcacaaat tgtctactcg
     6301 gcggccgagg tatacggtcg ctcggatgta gtcgtaaagg tcactcgtcc tacagcgact
     6361 gagcacgcgc tatttcagcc gggacaaacg ttgatagcct ttctccatat ggctgtggcc
     6421 tcgcccgact tcctggaagc gctgcgcgaa aaggagatca ccgccattgc ctgtgagatg
     6481 atccgccagc caaacgggaa tcatcctatc ctggtgccca tgagcgaagt cgccggccgc
     6541 ctggcgcccg tcatcgccgg gcagcttctc atgacgaacg gcggcggccg aggcacgctg
     6601 ctgagcggta tacccggagt gcctcgcggc tctgtcgcca tcatcggcgg cggggtgctt
     6661 ggtgcgaatg cggcgcgcgc atttctgaat gccggtgctc aggtcgttgt gctggacaac
     6721 gatatcgcac gtctgaaaca cctggatgag gccctgag
//
LOCUS       DAOMQG010000167         6749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4843_length_6749_cov_9.187481, whole genome shotgun sequence.
ACCESSION   DAOMQG010000167 DAOMQG010000000
VERSION     DAOMQG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4843_length_6749_cov_9.187481"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLC06_09960"
     CDS             complement(<1..407)
                     /locus_tag="PLC06_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRO90811.1"
                     /translation="MSDRIIEPRTVLPRDEFLAGVRAELPILLGVIPFGLIFGAVAVS
                     AGLPVSLAQAMSVVIFAGSAQFIAAELIGVGTPALVLLLTTLIVNLRHLLYSASLAPH
                     VNKLPLRWKMLLAYLMTDEAYAVTIIHYTEGRTP"
     gene            complement(429..3407)
                     /locus_tag="PLC06_09965"
     CDS             complement(429..3407)
                     /locus_tag="PLC06_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRO90812.1"
                     /translation="MIKRFTCRIGLSLALLSAAAIMLGGCDPGGRLTQLTPQPLPGTP
                     ILLGVTAEAPGGGTLATAAPQAIEGPLPVALPSPTYDAALPAWTILYYTGADNGRARF
                     VWDDLNEMEAAGTLDQVRVVAQVDWPEGGPTATGETIRYAITADNDKTQFTSPPVAAL
                     GESNMGDPAVLADFLTWGMATYPANRFALILGDFGGGWQGCCYDQTLPTPGQTDHLSL
                     PDIDQALALARGANGKRLEVIAFTASLMNQIDVLQTVQPYAAYAVASAGLVPGSSWDF
                     EPVLTQLNADPLIDGRRFSGDLVTAFVNYQRQLARDEYSGMAAVDLAQVPALSTAVEA
                     LALAIVDDPELYGAIVAEARRGAQQYGTAAITDAERIAAVDLLHAAALITEITPPGGV
                     QTAATAVTSAVTSAIVAYDHGLGIPYGRGVAIYWPASPAQFDPLYVQVTRLPSWATFV
                     ATIPSGATIAPQVRVDRGSRDTINIGRPGLLRSEVLGRQITTIALVADQEAADGRRVL
                     RQFEIIQPASITLPGGTTASVWRDGQHESLIVWDGTAGFLADSAGTGDFATFQAVDFS
                     SIGPQLATRGVFRRAGSETGMEATATFSNAEPASRHTWAVGDVSSGTRLIGEISPTAG
                     DTFESAITIAGLDGTTTSEPGVTLTFDAAPAIYRTTRPLPGGKYGVGLRVESVGNSPV
                     TATQPLSINDDGLIPGFRAFVDIARNIQFLYPADWVPPVAQDGITYTGNISGTAQLQV
                     RYYPNWASDLSALHSEVLGTFGEVSVLQQESIQVGTEAPVDAVRTAYGYDSADQGPRT
                     GIFLTFIKDGTGYVVDMDGPREQEAATLEAVNTIATTWQFLPQRLGFGPEPWSVLNVG
                     DFRMKYPTGFAYQEFNSWHRFAADPSTFVAVRIQPAGRTAAEAMTSLLQTAAEGVTGF
                     TAGEPERYFYAGHVWERNDFHYTDTNGNIVAGLLLSRQEGNAEIAVWAEAPDPADELF
                     QSVFLPTAATIERIPPPPSG"
     gene            3593..4702
                     /locus_tag="PLC06_09970"
     CDS             3593..4702
                     /locus_tag="PLC06_09970"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRO90813.1"
                     /translation="MSNKWHHYSGNMHIHTRYSDGEKLHAQIAEDAIAAGLDFIIVTD
                     HNVHVSGVEGYYENENGRVLLLTGEEVHNVRRRPQASHFLAYGVGKELATYASDPQAL
                     IDETLNAGGYGFLAHPHERDLPLINMPDLGWYDWDIEGFSGLEIWNYMSSFKNTVAER
                     LSELRWKNKFISGLVALRMTLHPERYIKGADPRTLALWDDFLAKGTRIAAVGNSDAHG
                     TPMKLGPLRREVYPYEFLFRAVNTHVLIDRPLTGDLEHDRRLLIRAIGRGYSWVGYDM
                     PGSTNEFRFTVQGVRSGVMGDEMPLSTGATLQIKAPSRCRIRVIRHGEVVAEVERESN
                     LTYIPDEPGAYRVECHTFYEGEERGWIYSNPIYLR"
     gene            complement(4714..>6749)
                     /locus_tag="PLC06_09975"
     CDS             complement(4714..>6749)
                     /locus_tag="PLC06_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRO90814.1"
                     /translation="LGAAALFFIVFVAMLYLATGEKAYTLSGLTLLLLAGVIAFYAFD
                     TVVAPRVLSWLNPWPNVSDRAYQIVQALYAQAAGGILGQGIGQGSPDYIPVVHSDFAL
                     AAIAEEWGLIGSLTVVGCFVVLAVRGLRIAILSIRNPRPQFFHAYLAAGLVALFSVQA
                     LLIMGGVTRLLPLTGITLPFVSYGGSSLLVSNLAIGMLFFISAQAESASATPTADPGL
                     ARRIDRLGRVILICFAIVAGALTYWSTLRSDSLLARADNPRLVENERNIQRGLIMDRD
                     DVILAETVVTANRRERRYPLPDSGPAVGYYSIRYGSAGVESAFNDHLSGRDNNLWVNA
                     LRRLLHEPVIGRDIGLTLDAGLQQMAAHAMATTGITGGMVLLEHVSISGDPVAEIRAM
                     VSQPGYDPNLIDAEFEELSVIEPGPLVNRATQGLYQPGLVLQPFITAAAMETDKLEPA
                     TPLDDPFRPVAINGQVIRCMLPRDESAEAPLTWADMGRLRCPAPLKALGEQLGAPALQ
                     AILEGFGFGQTPEMPVTMVTQAVRIQDVGLAAIGQDVLTITPLQAARATAALIGDGRL
                     PGLRLVERLQGSDKSWATQPPGAPASSTSLISRGTAAIIREGWETDEGALAFSALVLA
                     RPDGGRNAWFLGMAPADNPRFVLALVLEDVDDTEAAEELGREILLGATASQGE"
BASE COUNT         1411 a         1946 c         2057 g         1335 t
ORIGIN      
        1 ggtgtgcggc cttcggtgta gtgaatgatc gtcacggcat aggcctcgtc ggtcatcaaa
       61 tacgccagca gcatcttcca gcgcaacggt aacttgttca catggggtgc cagcgaggcg
      121 ctgtagagca gatgtctcag gttgacgatt aaggtcgtca gcaacaggac aagcgccggt
      181 gtaccgaccc caatgagttc tgcggcgatg aattgtgcgg atcccgcgaa gatcacgacc
      241 gacatggcct gcgccaggga gacaggcaga cctgccgata cggccaccgc cccgaagatt
      301 agcccaaagg gaatgactcc cagaaggata ggtaactcgg cgcggacgcc cgccaggaat
      361 tcgtcgcgcg gtaatacagt tctcggttca ataatacgat cactcattaa agacccaggt
      421 tgttgaaatt agccggaggg tggtggagga attcgttcga ttgtggcggc cgtcggcaag
      481 aagacagatt ggaacagttc gtcggcggga tcaggagctt ctgcccatac tgcaatctcg
      541 gcattaccct cttgccggct cagcagcaat ccggccacga tattgccgtt ggtgtcggtg
      601 taatggaagt cgttgcgctc ccaaacgtgg ccggcgtaga aatatcgttc cggctcgccg
      661 gcggtgaacc ccgtgacccc ctcggcggcc gtttgcaata aactcgtcat cgcctcggcg
      721 gccgttcgtc ccgcgggctg gatgcgaaca gccacgaatg ttgacggatc ggcggcgaac
      781 cgatgccagc tgttgaactc ctgataagca aaaccggtcg gatatttcat ccgaaaatcg
      841 cccacattca ggactgacca gggttccggc ccaaagccga gtcgttgcgg cagaaattgc
      901 catgtcgtgg cgatggtatt cactgcctcc agtgtcgcgg cttcctgttc gcgcgggcca
      961 tccatgtcga cgacgtaacc tgttccgtcc ttgataaatg taaggaatat gcctgtgcgt
     1021 ggcccctggt cggcgctgtc atacccataa gccgtgcgaa cggcatcgac gggagcctct
     1081 gtgccgacct ggatcgattc ttgctggagg acactcacct cgccgaacgt gcccaatact
     1141 tcgctatgga gcgcgctcag atcggaagcc caattggggt aatagcgaac ctgcagttgg
     1201 gctgtgccgc tgatgttgcc ggtgtaggta atcccgtcct gggctaccgg cggaacccaa
     1261 tcggctggat acaggaactg gatgttgcgc gcgatgtcga caaatgcccg gaatccgggg
     1321 atcagcccgt catcgttgat gctcaggggt tgggttgccg ttacagggct gttgcccacc
     1381 gattcgacgc gcaatccgac accatatttg ccgccgggta atgggcgagt cgtgcgataa
     1441 atcgccggcg ccgcgtcgaa agtcaaggtg acccccggct cggaggttgt cgttccatcc
     1501 aacccggcaa tggtgatggc ggattcgaaa gtatctcctg ccgtgggaga gatttcaccg
     1561 atgagccggg tgccgctgct gacatcgccc acggcccacg tatggcgtga agccggttct
     1621 gcattgctga acgttgcggt ggcttccatg cctgtttctg atccggcacg gcgaaagacg
     1681 ccgcgcgtcg ccagctgtgg cccgatagag gagaaatcta ccgcctgaaa ggttgcaaag
     1741 tcacccgttc cggcagagtc ggccagaaac ccggctgtgc cgtcccagac gatgagcgat
     1801 tcatgttgcc cgtctcgcca aacagatgcc gttgttcccc ccggcagcgt gattgatgcc
     1861 ggttgaatga tctcgaactg ccgcaagacg cgccgaccat cggccgcttc ctgatcagcc
     1921 accagggcga tcgtagtgat ctgccggccc agcacctcgg aacgaagcag gcctgggcgg
     1981 ccgatgttaa ttgtgtcgcg actgcctcta tcgacgcgaa cttgcggggc aatggtggct
     2041 cctgatggga tcgtggccac aaacgtggcc cacgacggga gtctcgtcac ctgtacgtac
     2101 agggggtcga attgagcagg cgaagccggc cagtagatgg cgaccccacg gccatagggg
     2161 atgccgaggc cgtgatcata ggcgacgata gccgaggtca cggccgaggt tacagccgtt
     2221 gccgcagtct ggacgccgcc gggcggcgtt atttcggtga tgagggcggc ggcatgaaga
     2281 agatcgacgg ccgcgatccg ttcagcgtcg gtgatggccg ctgtgccata ctgttgcgcg
     2341 ccgcgccgcg cctcggcgac gatcgcccca tatagttcag gatcatcaac gattgccagc
     2401 gccagcgctt caaccgcggt cgagagtgcc ggaacctggg ccagatcaac ggcggccatg
     2461 cccgaatact catctcgggc caactgccgt tggtaattga cgaaggcagt cacgagatcg
     2521 cccgaaaatc ggcgtccgtc aatcaatggg tcggcattca gttgggttaa aacaggctca
     2581 aaatcccatg acgaacccgg aaccagcccg gccgatgcca cggcatatgc cgcatagggc
     2641 tggaccgttt gcaatacgtc gatctgattc atcagacttg cggtgaaggc gatgacctcc
     2701 agccttttcc cgttcgcccc ccgcgccagc gccaatgcct gatcgatatc cggcaagctc
     2761 aggtgatcag tctggcccgg tgtgggcaag gtctggtcat agcaacatcc ctgccagcct
     2821 ccaccaaaat caccgaggat gagggcgaag cggttggctg gatacgtggc catgccccag
     2881 gtcagaaaat ctgccagcac ggcgggatcg cccatattgc tctcgccgag ggcggcaacc
     2941 ggtggagatg tgaattgggt cttatcgtta tccgccgtaa tcgcgtagcg aatggtttcg
     3001 ccggtcgcgg tcgggccacc ttcaggccaa tctacctgag ctacaacgcg cacctgatcg
     3061 agtgtgcccg ctgcctccat ctcgttcaga tcgtcccaaa caaagcgcgc ccgaccgtta
     3121 tccgcacccg tataatagag gatagtccag gcgggcaaag ccgcatcata ggtgggggat
     3181 ggtagcgcca ccgggagcgg gccttcgatc gcctggggag cggccgtggc cagcgttccg
     3241 ccgccgggtg cttcggctgt cacgcccaac aagataggcg ttccgggtaa tggttgcgga
     3301 gtcagttgtg tcaatcggcc gccgggatcg caaccgccaa gcataatggc ggcggcgctc
     3361 aataacgcga gactcaaccc tatccgacag gtgaagcgct tgatcattag acataaaatg
     3421 tagcatagaa accggcggag aggcaattgc atcggccgca acagatgaaa acaagcgctt
     3481 ttcaatagag gctttcgcgg ggtgacgatc gcgccattca tggttttatt tgttgtcgct
     3541 ccgcatacgg agtagcatac aaacctacta tcagccttat tttaggaacc ttatgtcgaa
     3601 taagtggcat cattacagtg gcaacatgca catccatacg cgctactccg acggcgaaaa
     3661 gttgcatgcc caaattgccg aggatgccat cgcggcgggt ctcgatttca tcattgtcac
     3721 cgaccataac gttcacgtat cgggggtgga ggggtattac gaaaacgaga acggccgcgt
     3781 actgctcctg accggggaag aggttcataa tgttcgccgc cggccacagg cgagtcattt
     3841 tctggcttac ggtgtgggca aggagttggc gacctacgca tccgaccctc aagcgttgat
     3901 cgatgagacg cttaatgcag gcggctatgg gtttctggcc catcctcacg aacgtgattt
     3961 gccactgatc aacatgcccg acctgggttg gtacgattgg gatatcgagg gcttttcggg
     4021 gctggaaata tggaactaca tgtcttcctt caaaaacacg gtggccgagc ggctgagtga
     4081 gcttcgttgg aagaataagt tcatcagcgg gcttgtggct ttgcgcatga ccttgcaccc
     4141 ggaacgctat atcaaaggcg cagatccgag gacgctggct ctatgggacg actttctggc
     4201 caagggaacg cgaattgcgg ccgttggtaa cagcgatgcc cacggcacgc cgatgaaact
     4261 ggggcctctg cgacgcgaag tgtaccccta tgagttttta tttcgcgccg ttaacacaca
     4321 cgtgctgatt gacaggccac tgactggcga tcttgagcac gatcggcgct tgctcattcg
     4381 ggcgattggt cgtgggtata gctgggtcgg ctacgacatg cccggctcaa cgaatgaatt
     4441 tcggttcacg gttcaggggg tgagaagtgg tgttatgggg gatgagatgc cgctgagcac
     4501 cggtgcaacg ctgcaaatca aggccccaag tcgctgccgt attcgtgtta ttcgccatgg
     4561 tgaggtcgtc gcggaggtcg agcgagagag caatctgact tatatccccg acgagccggg
     4621 cgcctatcgg gtcgaatgtc acacattcta tgaaggtgag gaacggggct ggatatatag
     4681 caatccgatt tatctgcgtt aattcggttc tccttactct ccttgcgagg ccgttgcgcc
     4741 taacaatatt tctcgtccca actcctcggc cgcctcagtg tcatccacat cttccagaac
     4801 cagcgcgaga acaaaccgcg gattgtcggc cggggccatg cccaggaacc aggcgttgcg
     4861 gccgccgtcc ggtctggcca acaccagcgc gctaaaggcc aaggctcctt cgtcggtctc
     4921 ccatccctcg cgaatgatcg cggctgttcc ccgtgaaatg aggctggtgg atgacgccgg
     4981 cgcgccaggg ggttgggtcg cccaactctt gtcgctcccc tgcaagcgtt caaccagacg
     5041 aagaccgggc aggcggccgt ctccaattag cgcggccgtc gctcgtgccg cttgcaacgg
     5101 tgtgatggtg agcacatcct ggccgatcgc cgccaggccc acatcctgaa tacggacagc
     5161 ctgagtgacc attgtaaccg gcatctccgg tgtttgaccg aagccgaacc cttccaggat
     5221 cgcttgcaat gccggtgcac cgagctgctc accaagcgcc ttaagcgggg ccgggcagcg
     5281 cagacgcccc atgtccgccc aggtgagcgg ggcttccgca ctctcgtcac gcggtaacat
     5341 acaacgaatt acctgaccgt tgatagcgac ggggcggaat ggatcatcca gcggagttgc
     5401 cggctccagc ttgtcggttt ccatcgctgc ggccgtgatg aacggctgca ggaccaggcc
     5461 gggctggtat agtccttgcg tggcgcggtt gaccagtggg cctggctcaa tcaccgataa
     5521 ttcctcgaat tcggcatcga ttagattggg atcatagccc ggttggctga ccatcgctcg
     5581 aatttcggcc accggatcgc cactaatgct gacgtgctcc aacaggacca tcccgccggt
     5641 aataccggtt gtggccatgg cgtgggctgc catctgttgc agaccggcat cgagggttag
     5701 accgatgtcg cggccgataa cgggttcatg gagcaggcga cgcagggcgt tgacccagag
     5761 gttgttgtcc cgaccactga gatgatcgtt gaaagcactc tcgacgccgg cgctgccata
     5821 gcggatgctg tagtagccga cagccgggcc gctgtcggga agagggtaac gtcgttcgcg
     5881 tcgatttgcc gtgactactg tctcggcgag aatgacgtcg tcccgatcca tgatcaggcc
     5941 cctctgtatg ttgcgctcgt tctcgacaag acgcgggttg tctgcgcgtg ccaggaggga
     6001 atcgctgcgc agggtgctcc aataagtcaa tgcccccgcc acgattgcaa agcagatgag
     6061 aatgacccgc cccagccggt cgatgcggcg cgccagcccc gggtcggccg tcggcgtggc
     6121 cgaggcagat tccgcttggg ccgatataaa gaacaacatg ccaatcgcca gattactgac
     6181 gaggagtgag ctgccgccgt aactcacgaa cggtaacgtg ataccggtca ggggcagtag
     6241 tcgcgttacg ccacccatga tcagaagagc ttgcacgctg aagagggcca caaggccggc
     6301 ggccagatag gcatggaaga attgcggccg tgggttgcga atggacagaa tggcgatgcg
     6361 cagcccgcga acggcaagga cgacaaaaca accaacgact gtcagactgc cgatcagccc
     6421 ccattcctcg gcgatggccg ccagggcaaa atccgagtgg acgaccggga tatagtcggg
     6481 cgatccctgg ccgataccct ggcccaggat gccgcctgcc gcttgcgcat atagggcttg
     6541 tacaatctga taggcacgat cgctaacgtt gggccagggg tttagccaac ttagcacgcg
     6601 cggcgcgacc accgtatcga aggcataaaa ggcgatgacc ccggccagta gcaggagagt
     6661 gaggccactc aatgtatacg ctttttcgcc cgttgccaga tagagcatgg ccacaaagac
     6721 gataaaaaat agcgccgcgg ctcccagat
//
LOCUS       DAOMQG010000168         6737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4857_length_6737_cov_10.191410, whole genome shotgun sequence.
ACCESSION   DAOMQG010000168 DAOMQG010000000
VERSION     DAOMQG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4857_length_6737_cov_10.191410"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLC06_09980"
     CDS             complement(<1..260)
                     /locus_tag="PLC06_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO90815.1"
                     /translation="MRVLMVAPTSFFSDYGGHIRILEEMRSLGELGVEVAVVTYYMGK
                     DVPGIDIRRTAPLPWRTDYEVGSSRHKIAFDAYLAVKTAREVL"
     gene            complement(303..1295)
                     /locus_tag="PLC06_09985"
     CDS             complement(303..1295)
                     /locus_tag="PLC06_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRO90816.1"
                     /translation="MKAQTVTIVGMSRTGLSIALALKASGSDLTIVGYDRFRELTQKA
                     KDETGAIDKAEWNLVNAAAEADILVLAVPLAELETNLMVIGDVLKPHALVLDLSVLKG
                     HGLRWADRHLRQGHYVGAVAVLSAAFLNDGRDEPGAATPDLFKGGMFCLMPSVRAEPR
                     AVDTAVSFGRLLGATPYFVDPMEYDSLVQGVETLPGLAAAALFGALQKSTGWRDMLRF
                     ANSSFALATQPLQNGTDITSLALNDKAATLRWLDAYLQELAELRALIMNGDREMIDLA
                     MGNLLIQREKWLQERVRDDWAEVTETKVEPPSISDQLVGGWLGGKLRRDHSDRS"
     gene            complement(1304..1822)
                     /locus_tag="PLC06_09990"
     CDS             complement(1304..1822)
                     /locus_tag="PLC06_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRO90817.1"
                     /translation="MRIGYRLRQLRANLAAGPLSESARREVVEVLTASEPALFDQYAP
                     ADQWHSYRVLRCLRDAGYNHPDLLTAALLHDIGKAQCPLSIWDRTVIVVVGALFPRRA
                     ERWGQGAPDGRRRPFVARAQHAEWGAQMAEAAGSAPLVIELIRRHQDKHVPAQTEADR
                     LLAILQWADDQN"
     gene            complement(1824..2456)
                     /locus_tag="PLC06_09995"
     CDS             complement(1824..2456)
                     /locus_tag="PLC06_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRO90818.1"
                     /translation="MFNLDIPTIIERVIVLLIAFTVHELAHAVTADYLGDPTPRRMGR
                     ITLNPLKHLDPFGTIMLIIAGFGWAKPVMTNPMNMRGNPRTSMAIVAFAGPLSNLVMA
                     GIAAIFFRLGLIDINVLMSGGGALSFSSLVFQFLYINLALAFFNLLPVPPLDGSRILY
                     AILPAEMVYRLRPLEQYGFMILFLVVFFVPQVLNVLVWTPVQATARLLIG"
     gene            complement(2547..3608)
                     /locus_tag="PLC06_10000"
     CDS             complement(2547..3608)
                     /locus_tag="PLC06_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO90819.1"
                     /translation="MPILEVVGINKFYPPDTYAVRNVDIRLERGEILCLLGPSGCGKT
                     TLLRMIAGLERPDSGAILFEGRDIINVPPHERGFGMMFQDFALFPHKNVRDNIAFGLR
                     MKKLPPKEIAERVARILDIVELDGLADRTISQLSGGEQQRVALGRALAPGPRLLMLDE
                     PLGALDRALRERLMLDLRTILKRVGMTAVYVTHDQTEAFSVGDRLVVMDGGRIIQTGS
                     PREVYENPATPFVARFLGFQNLVYGRIDAAGMVDTEVGRFRMSQPVPAPGSEVLLLIK
                     PMMSGAEIRPLPAEPESNTIYGIVDAVTFRGRFSQIGIATGSHRLLFEESGDLPFVLG
                     DEISVAIPPGHLRLYEASL"
     gene            complement(3613..5346)
                     /locus_tag="PLC06_10005"
     CDS             complement(3613..5346)
                     /locus_tag="PLC06_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRO90820.1"
                     /translation="MDTHPGRSRLIGSARSIAVVAIFLLPAVFFALFYLYPLGSILRL
                     GLMPEGRLNTAALLQPLVNPNLLRIVWFTTWQAFVSTVLTLLLAFPGAYVLARFSFRG
                     KTLVRTLATLPFVLPTVVVAAAFIALIGPSGVINNWLRDLLALDRGPIALSQTIWIIL
                     LAHVFYNYSVVLRLLSAYWQNLPPSMTQAAQMLGASPLRAFFEVTLPLLRPAITSAAT
                     LVFIFCFTSFGVIIILGGPRFATIEVEIYRQAVNLFNLPLAAALSLWQIIFTFILMWV
                     YTRTQARLGQTVKASAGKAAERPVRTPREKWLVYGNVVAIVILIGAPLLALVARSFRG
                     QRGPTLLYYRSLFINQDDSLFFVPPGEAILNSILIAFGAMILAVSLGLLAATVLSRWE
                     RNAAGNGRLGALIPRTLDALLMLPLATSAVTLGLGYLISLGRPPLNLRDSIIILPIVH
                     AVVAVPFVMRAVLPGFRGIQPSLREAAAMLGADGRHVWWEVDWPLLRRAILAGAIYAF
                     TISLGEFGATVFLARPETPTLPVAIYRFLGRPGALNYGQALAMSVLLMGVCAVAFVVI
                     ERFRFGNEGEF"
     gene            complement(5355..6410)
                     /locus_tag="PLC06_10010"
     CDS             complement(5355..6410)
                     /locus_tag="PLC06_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRO90821.1"
                     /translation="MKHVKVWILVLLLGVLGSACGSGDQTADTPAVVRLMTHDSFDVS
                     AELIASFEAETGLDVEIFKAGDGGEMLNKAILAGDAPLADVLFGVDNTFLSRALENDL
                     FLPYESPVLLEIRDGLELDPEYRVSPVDYGDICLNYDVAWFEEAQLPPPDSLEDLADP
                     MYKGLTVVENAATSTPGLGFLLATVERFGEDGYLDYWRQLVANDVLIVDGWEQAYYSY
                     FTAASEGDRPIVVSYASSPVAEVFYAEEPPASAPTAAVLTDGTCFRQVEFAGILRGTK
                     VEKEARLLVDFILDQQFQEDIPLKMFVYPANETAVLPELFQKYGHAADKPVQMDPARI
                     TANREAWIQAWTDVALR"
     regulatory      complement(6459..6571)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1567 a         2147 c         1862 g         1161 t
ORIGIN      
        1 aggacttccc tggcggtttt caccgccaga tacgcatcga atgctatctt gtgccgcgag
       61 gaacccacct cataatcggt acgccaaggg agaggcgcag tccgccggat gtcgatgccc
      121 ggaacgtcct tacccatata ataggtaacc accgcgacct cgacacccaa ctcccccagc
      181 gaccgcatct cctccaggat acggatatgg cccccataat cactgaagaa agatgtgggc
      241 gcgaccatca atacgcgcac gcgataactc ctgacccaat ggggctaaac cacatggtgc
      301 aattacgacc tgtcggaatg atcccgcctc agcttgccac ccagccaacc accgaccaat
      361 tgatcgctaa tcgacggcgg ctcgactttg gtttccgtca cttccgccca atcatcacga
      421 acgcgctcct gcaaccattt ttcccgctgg attagcaggt tccccatggc cagatcgatc
      481 atctccctgt cgccgttcat gatcagcgcc cggagctcgg ccaactcctg cagataggca
      541 tcgagccagc gaagcgtggc ggccttgtca ttgagcgcca ggctggtgat gtcggtgccg
      601 ttttgcaacg gttgcgtggc taaagcgaaa gaggagttgg cgaagcgaag catatcccgc
      661 cagccggtcg atttctgcaa tgcgccaaag agcgccgccg ccgccagacc cggcaaagtc
      721 tccacccctt gcaccaaact gtcatactcc atcggatcga cgaaataggg cgtcgccccc
      781 agcaaccggc cgaaactgac ggccgtatcc accgcccgtg gctcggccct gaccgacggc
      841 atcaggcaaa acatcccgcc cttgaacaga tcgggcgttg cggcccccgg ctcgtcacgt
      901 ccgtcattca ggaaggcggc cgacagcacc gccaccgcgc ccacataatg tccctgccgc
      961 aaatgccggt ctgcccaacg caggccgtgc cccttgagca ccgacaaatc aagcaccagg
     1021 gcgtggggct tcaacacatc gccgatgacc atcagattcg tttccagctc cgccaaaggc
     1081 acagccagaa ccaggatatc agcctcggct gcggcattaa cgaggttcca ttccgccttg
     1141 tcaatggctc ctgtctcatc tttcgctttc tgggtcagct cgcgaaagcg atcatagccc
     1201 acgatcgtca ggtcagaccc cgaagccttg agcgccagag caattgacaa acccgtccgg
     1261 ctcatgccaa cgatagtaac tgtttgcgct ttcataactc tctctagttt tgatcatctg
     1321 cccactgaag gattgccagc aacctgtctg cttcagtttg agcgggaacg tgtttgtcct
     1381 gatgccgccg aatcaactcg ataacaagcg gcgcgctgcc cgccgcctca gccatctgcg
     1441 cgccccactc ggcatgttgc gcgcgggcga caaacggccg tcgccggcca tcaggcgcgc
     1501 cttggcccca gcgctcggcc cgacgaggga acaaggcccc aacgacgaca atgacagtgc
     1561 gatcccatat ggagagtggg cactgcgctt tgcctatgtc gtgaagcaag gcggccgtta
     1621 acagatcggg gtgattatat cccgcatccc gcaggcaacg taacacccgg tagctgtgcc
     1681 attgatcggc cggggcatat tggtcgaaca acgccggctc gctcgccgtc aacacctcga
     1741 ctacttcgcg gcgcgccgac tcactcagcg gcccggccgc caggttcgcc cgaagctggc
     1801 gaagacgata accgattctc acgctaaccg atcagcaatc gcgcggtagc ctgaacgggt
     1861 gtccacacca gcacgttcag cacctggggc acgaagaaca cgaccagaaa gaggatcatg
     1921 aacccgtatt gctccagcgg ccgcaaacga tagaccatct ctgcgggcag aatggcgtac
     1981 agtatcctgg aaccgtccag cggcggtacc ggcaacaagt tgaagaaggc cagggcaagg
     2041 ttgatgtaga ggaattggaa caccaggctg gaaaatgaga gcgcgccgcc gccgctcatc
     2101 aggacattga tgtcaataag ccccagccgg aagaagatcg ccgcgattcc ggccatcacg
     2161 agattcgata gcggcccggc aaaggcgaca atggccatcg aggtgcgcgg gttgccacgc
     2221 atgttcatcg gattggtcat gaccggcttg gcccaaccaa aaccggcaat gatgagcatg
     2281 atggtcccga acgggtcgag atgcttcagc ggattcaacg taatgcgccc catgcggcgc
     2341 ggcgtggggt cgcccagata atcggctgtc accgcatgag caagttcatg gacggtaaaa
     2401 gcgatcagca agacgatgac tcgctcgata atagtaggga tatccagatt aaacatatct
     2461 ttgcgtaacg gactcaaccg gattagtcgc ggatctcgcc caagtataca ttttccttca
     2521 ttcagaaacg agcgccgggc cgatcatcaa agcgatgcct catacaggcg aaggtgaccg
     2581 ggagggatgg cgaccgatat ttcatccccc aacacaaaag gcagatcgcc tgactcctca
     2641 aataaaagcc gatgggaacc ggtggcgatc cctatctgcg aaaagcggcc gcgaaaagtg
     2701 actgcatcga cgatgccgta tatcgtgttg gattccggct cggcgggcaa cggacgtatt
     2761 tcagcgccgg acatcatggg cttgatcaac agcaacacct cgctgccggg cgcaggtacc
     2821 ggttgactca tccgaaatcg gccgacttcc gtatcgacca tgcccgcggc gtcaattcgg
     2881 ccatagacaa gattctggaa gcccaggaac cgggcgacga acggcgtggc cggattctcg
     2941 taaacttctc gcggcgagcc ggtctggata attcgccccc catccatcac caccagccgg
     3001 tcgccaacgg aaaaagcctc ggtctggtcg tgggtgacat agacggccgt catcccgaca
     3061 cgcttcagaa tcgtccgcag atcaagcatg agccgctcgc gaagggcgcg atccagcgca
     3121 cccagcggct catcgagcat cagcaggcgc gggccaggtg caagcgctcg ccccaacgcc
     3181 acacgttgct gttctccgcc cgagagttgg ctaatcgtcc tatcggccag gccgtcaagc
     3241 tccacaatat cgagaatcct cgcgacacgt tcggctatct ctttgggcgg caacttcttc
     3301 atgcgcagcc caaaagcgat gttgtccctg acgttcttgt ggggaaacag ggcgaaatcc
     3361 tggaacatca tgccgaagcc gcgttcatgc ggcggcacat tgatgatgtc tcgaccctca
     3421 aacaaaatag ccccactgtc aggcctttcc aggccggcga tcatgcgcag caaggtcgtc
     3481 ttgccgcatc cggaaggccc caacaggcaa agaatctccc cccgctccag ccggatatcg
     3541 acattgcgca ccgcgtatgt atcgggtgga taaaatttat tgataccgac cacttccagg
     3601 ataggcattg gcttagaact ccccttcgtt gccgaagcga aagcgctcga tcaccacaaa
     3661 agcgaccgcg cacaccccca tcaataacac gctcatggcc aacgcctgcc cataattgag
     3721 cgcgcccggc cgccccagaa agcggtagat ggctaccggc agggtcggcg tttccggtcg
     3781 ggccagaaag actgtcgccc cgaactctcc cagactgatc gtgaaggcat aaattgcccc
     3841 ggccagaatg gcgcgacgga gcaacggcca atccacctcc caccacacgt gccggccgtc
     3901 ggcccccaac atggccgccg cctcgcgcaa ggacggctgg atgccgcgaa agcccggcaa
     3961 caccgcgcgc atgacgaacg gcacggccac cacggcatgg acgattggca atatgatgat
     4021 cgagtcgcgc agattcaacg gcggccgacc cagcgagatc agataaccca ggccgagcgt
     4081 gacggcggag gtcgccagcg gcaacatcaa caacgcatcc aacgttcggg gaatcagcgc
     4141 ccccaacctt ccgttacccg ccgcgttgcg ctcccagcgg gacaacacgg tggcggccag
     4201 caaccccaaa ctcaccgcca ggatcatcgc cccaaacgcg atcaggatcg aattaaggat
     4261 agcctcgccc ggcggcacaa agaacaacga atcatcctga tttatgaaca gggagcgata
     4321 gtagagcagg gtcgggccgc gctgtcctcg aaaagagcgc gccaccaagg ccagcagggg
     4381 cgcgccgatc aggatcacaa tggcgaccac attgccataa accagccact tctcgcgcgg
     4441 cgtcctgacc gggcgttcgg ccgccttccc ggccgacgcc ttcaccgtct gccccaggcg
     4501 agcctgggtg cgcgtgtaga cccacatcag gataaaggtg aaaatgatct gccacaatga
     4561 cagagccgcg gccagcggaa ggttgaacag gttcacggcc tgccgataga tctccacctc
     4621 gatcgtggca aaacgcggcc cccccagaat gatgatgacg ccaaagctgg tgaagcagaa
     4681 aataaacacc agcgtggccg cggatgtgat ggccgggcgc agcaacggta acgtcacttc
     4741 gaaaaaagcc cgaagcggcg aagcgccaag catttgagcg gcctgggtca tggacggcgg
     4801 caggttttgc cagtaggcgc tgagcagtcg cagaacgacg ctatagttat agaagacatg
     4861 ggccagcagg atgatccaga tcgtctggct gagcgcaatt ggtcccctgt cgagcgcgag
     4921 cagatcgcgc agccagttgt tgattacgcc actcgggcca atgagcgcga tgaacgccgc
     4981 ggccacgaca acggtgggca ggacgaaagg aagcgtcgcc agtgtgcgaa caagggtctt
     5041 gccccgaaac gagaaccgcg ccaggacata cgcgcccgga aaagccagca gcaaggtcaa
     5101 tacagtagac acaaaagcct gccacgtggt gaaccacacg atccgtaaaa ggttcgggtt
     5161 gaccaagggc tgcaacaggg cggccgtgtt caaccgccct tccggcatca gccccaggcg
     5221 caatatcgac cccagcggat agagatagaa cagcgcaaag aagaccgccg gcagcaggaa
     5281 tatggcaacg acagcgatgc ttcgcgccga accaatcaat cgagagcggc cagggtgcgt
     5341 atccacagat tcacttatcg caaggcgaca tcggtccagg cttgaatcca ggcctcacga
     5401 ttcgccgtga tccgggccgg gtccatctgc acaggcttat ccgcggcgtg gccatacttc
     5461 tggaataatt ccggtagcac ggccgtttca ttggccgggt agacgaacat cttcaggggg
     5521 atatcctcct ggaattgttg atcaaggatg aagtcgacca acaaccgggc ctctttctcg
     5581 acctttgtcc cgcgcaggat gccggcaaac tcaacctgtc ggaagcaggt tccatcggtg
     5641 agcacagccg ccgtcggcgc ggaagccggc ggctcctccg cataaaatac ttcggcaacg
     5701 gggctgctgg catagctgac tacaatcggc cgatcgccct ccgacgccgc ggtaaagtag
     5761 ctgtagtaag cctgttccca gccatcgacg atgagaacat cgttggcaac cagttgccgc
     5821 cagtagtcca ggtagccgtc ctcgccaaaa cgctcgacgg tcgccagcag aaagccaagt
     5881 cccggcgtag aggtcgccgc gttctcgacc accgtcaggc ccttatacat cggatcggcg
     5941 agatcctcca ggctatccgg cggaggcagc tgggcctcct cgaaccaggc cacgtcgtaa
     6001 ttcaggcaga tgtccccata gtcaaccggc gagacgcgat actccgggtc aagctccagc
     6061 ccgtcgcgga tctccagcag caccggcgac tcataaggca aaaacaggtc attctccagc
     6121 gcgcggctca ggaaggtatt gtccacccca aacagcacgt cggccagagg ggcgtccccg
     6181 gccaggatag ccttattcaa catctcgccc ccgtccccgg ccttgaatat ttcgacgtca
     6241 agcccggtct cggcctcgaa actcgcgatc agctccgcgc tgacgtcaaa gctgtcatgg
     6301 gtcatcaggc gaacgacagc cggcgtatcg gccgtttggt cgccggagcc gcaggcgctc
     6361 cccagaaccc ccaaaagcaa caccagaatc cataccttaa catgcttcat atagcatctc
     6421 tccaaaaaat aaagcgcccc tggttgtggg gcgcttgaaa cgatttccag gtcatttccc
     6481 tacgccagca taacctggat caggttgagc ggtttgtcca atagtcagga acatctcagc
     6541 ccggtttcac cgtgcacccg caagacatct atgcagtttt atacagacgc tcattatagg
     6601 ttgccgggcg agcggcgtca attcgatttg acttttcgga gctatcgcct ataatcggtc
     6661 ttgttgttgc ggggtagagc agaggcagct cgtcgggctc ataacccgga ggtcgcaggt
     6721 tcgaatcctg tccccgc
//
LOCUS       DAOMQG010000169         6692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4902_length_6692_cov_13.828085, whole genome shotgun sequence.
ACCESSION   DAOMQG010000169 DAOMQG010000000
VERSION     DAOMQG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4902_length_6692_cov_13.828085"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="recA"
                     /locus_tag="PLC06_10015"
     CDS             complement(<1..841)
                     /gene="recA"
                     /locus_tag="PLC06_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRO90822.1"
                     /translation="MSIDQAKARALENTVATLTKRFGDGIIMRMGQATHLVVESIPTG
                     ALSLDIALGVGGVPRGRIIEIYGPESSGKTTLCQHIVAEAQKLGGICAFIDMEHALDP
                     SYAAKCGVDVDNLYVSQPDTGEQALEIADALIRSGAMDVVVIDSVAALVPRAEIEGEM
                     GDTHVGLQARLMSQALRKLSGVIKQTNTVVIFTNQLREKIGVMFGNPETTPGGNALKF
                     YASVRLDIRRIQAIKAGNDVTGNRTRVKVKKNKVAPPFTECEFDIMYNEGISRTGDVL
                     DLAI"
     gene            950..1879
                     /locus_tag="PLC06_10020"
     CDS             950..1879
                     /locus_tag="PLC06_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO90823.1"
                     /translation="MQLANSVEGNSSAAGGVRNASRQDAAAVHKLLRMGVYVHLHIDW
                     RLPGEWLDRPGFVVCEQGGGVGNAGESGSSDDDSPIRIIGCMAVAAEPLPAAWVRVAA
                     VDSVAGFAQAEAMFAAILAGLDPAINEIAWFLTDYWPLHWLERLGFVPISNVISYRKE
                     GLSVPDFTPPPGLEIRPLLIEDIPALAAMEAAAFEPRWRHSDDDLFRAWRQSLSFTVA
                     LLGGEPVAFQFSTGGDGNAHLSRMTVHPNKQGLGIGAALLADAMTGYRLQNITTVTLN
                     TQADNWPSQRLYERFGFVQTGSSYPVWSYSPKR"
     gene            1918..2214
                     /locus_tag="PLC06_10025"
     CDS             1918..2214
                     /locus_tag="PLC06_10025"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRO90824.1"
                     /translation="MTHKKLDDDEIRQALAQLPGWSVVDGKLHKEFKFGSFAEAIGWM
                     VSVAIFADKIDHHPEWLNVYNRVRVNLMTHDLDDAISDLDIEMARKMNTIAGQG"
     gene            2220..3221
                     /gene="argF"
                     /locus_tag="PLC06_10030"
     CDS             2220..3221
                     /gene="argF"
                     /locus_tag="PLC06_10030"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011261198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRO90825.1"
                     /translation="MSFNLRNRSFLKELDFTPEELHFLLKLSADLKSAKYGGYERPRL
                     VGKNIALIFEKASTRTRCAFEVAAHDQGAQVTYLGPSGSQIGNKESMKDTARVLGRMY
                     DGIEYRGFAQQTIEILAQYAGVPVWNGLTNEFHPTQLLGDVLTMSEHSDKRISEISYC
                     YMGDAHNNMGNSLMVGGCKLGMDVRICAPRHLWPAAELVNASRGIAAETGARLTLTEQ
                     VEEGVAGVDFIHTDVWVSMGEADAVWEERIRLLSPYQVNAGTMQLSGNPAVKFMHCLP
                     SFHNRETKIGEEIYQKFGLDGMEVTEDVFESPASIVFDQAENRMHTAKAVMVATLGA"
     gene            complement(3357..3623)
                     /locus_tag="PLC06_10035"
     CDS             complement(3357..3623)
                     /locus_tag="PLC06_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90826.1"
                     /translation="MDNIEAVKSLLKKLSALRATIPDDEQLYLDKLIMGTVKVKLPVD
                     PFEPTNITANKTASHQNPEQCNPTSPNPERRIAWNNDLEIYKLE"
     gene            complement(3743..>4298)
                     /locus_tag="PLC06_10040"
     CDS             complement(3743..>4298)
                     /locus_tag="PLC06_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRO90827.1"
                     /translation="QNDEADSLYFLYSGVMSVILISLDGREMIINELHPGDCFGEVSL
                     LTSGARTASAQARKSCVVLEVAANVFLNVLDSEPAVARRMLAVASDRLFKAQARESAL
                     AFLDAPSRIARALLDMDELDRQGPDHGYISISQEELAQRTGLTRQTVTNSLGQWRQRG
                     WIVTGRGRIMLLNRAALKRITEQI"
     assembly_gap    4299..4398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4399..4596)
                     /locus_tag="PLC06_10045"
     CDS             complement(<4399..4596)
                     /locus_tag="PLC06_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRO90828.1"
                     /translation="MTIKGEYPDKAEFLRSNPLLDGISESNLIRLGNASHYVNVSKGT
                     YLFNQNDEADSLYFLYSGVMSV"
     gene            complement(4723..6486)
                     /locus_tag="PLC06_10050"
     CDS             complement(4723..6486)
                     /locus_tag="PLC06_10050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRO90829.1"
                     /translation="MPAEFKPTRWSLEDIVPEPVASTLESLLADLDKRATAFESVRAT
                     LTDTMEAAEFQRIIGDYEAILREAYRAYGYVQLLFFSDLRSQTALNLRDRIDQVLTGI
                     NNRTLFFTLWLKGLSPETVERLAPTGDYRYYLQNLLKYKPYTLGESEEQIINLKDTNG
                     INAIVRLYDMITSGFRFTAEVGGEKQTLTRDQIAALYRHPAPEARAAAYQELFRVYEE
                     NSTVLAQIYSHRVRDWYSENMTLRNYPAPISARNLDNDIPDEVVDVLLEVCRNNNDLF
                     QRYFSLKAGWLGIDKLRRYDIYAPLTASDKTFDFAESVDLIIDSFRRFSPEIADMAMQ
                     VFADQHIDAEIRPNKRSGAFCYSVLPGMTPWVHVNYTGKARDVATLAHELGHAIHGII
                     SGDHSLLTYHPSLPLAETASVFSEMLLTDRLLAVETDPAVRRDILANAIDDAYATVQR
                     QSYFTLFEREAHKKIMGGSMVHELGDAYLDNLREQFGDTVQLSDDFRWEWITIPHIYH
                     TPFYTYAYSFGQLLVLSIYQQYRLEGESFIPRYKKLLAYGGSEEPAKVLQEAGFDIAS
                     TEFWQGGYDVLRGMIDELEQL"
BASE COUNT         1397 a         1688 c         1986 g         1521 t
ORIGIN      
        1 caatcgccag gtcgaggaca tcgccggtcc tggagatccc ctcattgtac atgatatcga
       61 actcacactc ggtaaagggc ggagcaacct tgttcttctt gaccttgacc cgggtgcggt
      121 tgccggtgac gtcgttgccg gccttgatgg cctggatgcg acggatgtcg aggcggactg
      181 aagcgtagaa cttcaaggcg ttgccgccgg gagtcgtctc ggggttgccg aacatgacgc
      241 cgatcttttc gcgcagctgg ttggtgaaga tgacgacggt gttggtctgc ttgatgacgc
      301 cggagagctt gcgcaaggcc tgggacatga gacgcgcctg gaggccaaca tgggtatcgc
      361 ccatctcgcc ttcgatctcg gcccggggga cgagggccgc gacggagtcg ataacgacga
      421 catccatggc gccggagcgg atgagggcat cggcgatctc gagcgcctgt tcgccagtgt
      481 cgggctggga gacgtagaga ttatcgacgt cgacgccgca cttggccgca taggaagggt
      541 cgagggcgtg ctccatgtcg atgaaggcgc agatgccgcc gagcttctgg gcttcggcga
      601 cgatgtgttg gcagagggtg gtcttgccgg aggactcggg gccgtagatt tcgatgatgc
      661 ggccgcgagg cacgccgccc acgcccaggg caatgtcgag agaaagagcg ccggttggga
      721 ttgattcgac aaccaggtgg gttgcctgac ccatccgcat gatgataccg tcgccgaaac
      781 gcttggtcag cgtggccaca gtgttctcca acgcgcgcgc ttttgcttga tcgattgaca
      841 ttctcgttac ggtagagcag acatcggatg gggctttatg cttccgcttg cgcctctatt
      901 tgcctcaagc aggataactt aagaacctta tgtagtgtac aatagccata tgcaattagc
      961 aaactctgtg gaagggaatt cgagtgcggc cgggggcgtt cgaaatgcct ctcgtcagga
     1021 tgcagcggcc gtccacaagc tgttgcggat gggtgtctac gtccacctcc acatcgattg
     1081 gcgactgccc ggcgaatggc tggatcggcc ggggtttgtc gtatgtgaac agggcggtgg
     1141 ggtcggcaat gcgggagaaa gcggttcgtc tgatgacgat tccccaataa ggatcatcgg
     1201 ttgcatggct gttgccgccg agccattgcc cgcggcctgg gttcgcgtgg cggccgtaga
     1261 ttcggtggcc ggctttgccc aggcggaagc gatgtttgcg gccatcctcg ccggcctcga
     1321 tccggctatc aatgagatcg cctggttttt gaccgactat tggccgttac attggcttga
     1381 acgtctcggg ttcgtgccga ttagcaatgt aatcagttac cgcaaggaag gtttatccgt
     1441 ccctgacttt actccgccgc ctggactgga gatccgcccg ttgctgattg aggatatacc
     1501 ggctctggcg gcgatggagg cggcggcgtt cgaacctcgc tggcgtcata gcgacgatga
     1561 tctcttccgt gcctggcggc agtcgcttag cttcactgtg gccctgctgg gcggtgagcc
     1621 ggtcgccttc cagttcagca ccggcggcga cggaaatgcc catctgtcgc gcatgacggt
     1681 tcacccaaac aagcaggggc tgggaattgg ggcggctctg ctggcggatg cgatgacagg
     1741 ctatcgcctg caaaacatca ctaccgtgac cctcaatacc caggccgata attggccgtc
     1801 ccaacgcctt tacgagcggt ttgggtttgt gcagaccggc tcatcctatc cagtatggtc
     1861 ctattccccc aagcggtaaa cgagccgtcg cacccttttt acaggaggct tccaaccatg
     1921 acccataaaa aacttgatga tgacgaaatc cggcaggcgc tggcccagtt gccggggtgg
     1981 tccgtcgttg acggcaaatt gcataaagaa ttcaaattcg gctccttcgc agaagccatc
     2041 ggctggatgg tgagcgttgc gatttttgcg gataaaatag accatcatcc ggagtggttg
     2101 aatgtttata atcgcgtgcg ggtgaatctg atgacacatg atctcgatga tgcgatcagc
     2161 gatcttgata tagaaatggc ccggaagatg aacacgattg ccggccaagg ataaaaagga
     2221 tgtcattcaa tcttagaaac cgcagttttt tgaaggaatt ggactttacg ccggaggaat
     2281 tgcactttct cctgaagctt tcggccgatc tcaagtcggc caagtatggc ggatatgagc
     2341 ggccccgttt ggtgggcaag aatatcgctc ttatattcga gaaagcgtcc acgcgcacgc
     2401 gatgtgcgtt cgaggtcgct gctcatgacc aaggggcgca ggttacctat ctgggtccgt
     2461 cgggatcaca aatcggcaat aaggagtcga tgaaagacac ggcccgcgtg cttgggcgga
     2521 tgtatgacgg catcgaatac cgtggcttcg cccagcagac aatcgagata ttggctcaat
     2581 acgccggcgt gccggtgtgg aacggtttga cgaatgagtt tcatccgact cagcttttgg
     2641 gagacgttct gaccatgagc gagcattccg acaagcgtat atcggagatc tcgtattgct
     2701 acatgggcga cgcgcacaat aatatgggta attcactcat ggttggcggc tgcaagctgg
     2761 gcatggatgt gcgcatctgt gcgccgcggc atctatggcc cgcggccgaa ttggtgaatg
     2821 caagccgtgg aatcgcggcc gaaaccgggg cgcggttgac cctgaccgag caggtagaag
     2881 aaggcgtcgc cggtgtggat ttcattcaca cggatgtatg ggtgtcgatg ggcgaggcag
     2941 acgctgtctg ggaagagcgc attcgtctgc tcagccccta tcaggtcaac gccggcacga
     3001 tgcaattgag cggcaacccg gccgtcaagt tcatgcactg cctgccttct ttccacaatc
     3061 gggagacgaa gatcggcgag gagatctatc agaagtttgg gttggacggg atggaagtga
     3121 ctgaagatgt gttcgaatcg ccggcgtcga tcgtatttga tcaggctgaa aatcggatgc
     3181 atacggctaa agccgtgatg gttgccaccc tcggcgcgtg atcttgcgac gtacctctgc
     3241 cggctgatgg ctcttgtaaa aggtgagagc cattaactca acagaaaagg ctcatgaatg
     3301 caagcgcacc catgagcctt ttttgttgag cgggaaaact tccgtataaa cttcgcctat
     3361 tccagtttgt aaatctccag atcgttattc caggctatcc ttctttcagg attaggcgat
     3421 gttggattgc attgttcggg gttttgatgg gaggccgtct tattggcagt gatgttcgtc
     3481 ggctcgaagg gatcgaccgg taactttact ttaactgtcc ccataatgag cttgtccaga
     3541 tagagttgtt catcatcggg aatcgttgcg cgtagtgccg atagcttctt tagcaaagat
     3601 ttgactgctt caatgttatc cactgctatt ttctccttgc ttccctaaag cccataccag
     3661 atggtaggac tactttacag gaaaatagca gccgttgtct gtcatctaga cgacacaaaa
     3721 cgcctaaaaa tcgattaatt tgtcaaatct gttctgtaat acgcttgagc gcagcacgat
     3781 tgaggagcat gatccggccg cgtcctgtca ctatccagcc tcgctgtcgc cactgcccca
     3841 ggctgttggt aaccgtttgt cgggttaacc cggttctctg ggccagttct tcctgagaaa
     3901 tcgagatgta tccatggtcc ggcccctgtc gatccagttc gtccatatcc agcaaggcgc
     3961 gagcgatgcg agagggggca tcaagaaatg ccagggcgct ttcccgtgcc tgggccttga
     4021 acagcctgtc ggaggccacg gccagcatcc ttctcgcaac cgccggctca ctatccagga
     4081 cattcaggaa gacgttggcc gctacctcca ggacaacgca gcttttccgt gcctgcgcgc
     4141 tggctgtgcg cgcccccgat gtaagcaggg aaacttcacc gaagcaatca cccgggtgga
     4201 gctcgttgat gatcatctcg cggccgtcca ggctgatcaa tatgacggac attacgcccg
     4261 agtagagaaa atacagggag tcggcttcgt cattctgann nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnga cggacattac gcccgagtag agaaaataca gggagtcggc
     4441 ttcgtcattc tgattgaaga ggtatgtccc cttggaaaca ttcacatagt gactggcatt
     4501 tccaagccga atgagattcg attcagaaat gccgtccaac aatgggtttg atcgcaagaa
     4561 ttctgccttg tcaggatact cgcctttaat cgtcattagc tcaacaagaa gttcgataac
     4621 gattcccgat tcaatgagcg acgctcatca gaaatccaca gataaataga taaaatgccg
     4681 gaaagcccga ctccatcgaa cgcagaggag atacgcccgg ccttagagct gttccagctc
     4741 gtcgatcatt ccccgcagca cgtcatatcc gccctgccag aattcggttg aggcgatatc
     4801 gaagcctgcc tcttgcaaca ctttggccgg ttcctctgat cccccgtagg ccagcaattt
     4861 cttgtaacgc gggatgaagg attctccctc gagccgatat tgttggtaga tggacaggac
     4921 gagcaattgc ccgaagctgt aggcataggt gtagaacggc gtgtgataga tgtgggggat
     4981 ggtgatccat tcccaacgaa agtcgtcgct caattggacc gtatcgccaa attgctcgcg
     5041 gagattgtcc aggtaagcat cacctagctc gtggaccatg ctcccgccca tgatcttctt
     5101 gtgggcttct cgttcgaaga gcgtgaaata ggattgacgc tgcacggtag cgtaggcgtc
     5161 gtcgatggcg ttggccagta tgtctctccg gacggccggg tcggtctcca cggccagcaa
     5221 acggtcggtc agcagcatct cggagaatac cgaggccgtt tccgccaggg gaagggaagg
     5281 gtgataggtc agcaaggagt gatcgccgga gataatgccg tgaatggcgt gacccaattc
     5341 atgagcgagt gtggcgacgt cgcgcgcctt gccggtgtag ttaacgtgga cccagggcgt
     5401 catgcccggc aggacgctgt agcagaaggc gccgctcctt ttattgggtc gtatctcggc
     5461 gtcgatatgt tggtcggcga atacctgcat ggccatatcg gcgatctcgg gcgagaaccg
     5521 ccggaagctg tcgatgatca gatcgacgct ttccgcgaag tcgaatgttt tatccgatgc
     5581 tgtcagcggg gcgtagatgt catagcggcg cagcttgtcg atgcccagcc aaccggcctt
     5641 caggctaaag tagcgctgga acaggtcgtt gttgttccgg cagacctcca ggagcacgtc
     5701 gacaacctca tcaggaatgt cgttgtcgag gttgcgggcg gagatggggg ccgggtagtt
     5761 gcgcaatgtc atgttttccg aataccagtc gcgtacccgg tggctataga tttgggccag
     5821 tacggtgctg ttctcctcgt atacccggaa cagttcctga taggcggccg ctctggcctc
     5881 gggtgcgggg tggcgataga gggcggctat ctgatcgcgg gtcagcgttt gcttctcgcc
     5941 gcccacttct gcggtgaatc taaacccgct ggtgatcatg tcgtaaaggc gcacgatggc
     6001 gttgatgccg ttcgtgtcct tcagattgat gatctgttcc tcggattcgc ccagcgtata
     6061 gggcttgtat ttcaggaggt tttggaggta gtaacggtaa tctccggtcg gggccaaacg
     6121 ttcgacggtc tccggcgata aacctttcag ccagagtgtg aaaaagaggg tgcgattgtt
     6181 gatgccggtc agcacctgat cgatccggtc gcgcagattg agggccgttt ggcttctcag
     6241 atcggagaag aacaagagct ggacatagcc gtaggcccga taggcctcac ggagaatggc
     6301 ttcgtaatcg ccgattattc gctgaaattc ggccgcttcc atcgtgtccg tgagggtggc
     6361 ccggactgat tcaaaggcgg tggcgcgttt gtccagatcg gctaacaggg attcgagcgt
     6421 tgatgcgaca ggttcgggaa caatatcttc gaggctccag cgggtgggct tgaattcagc
     6481 gggcattatc atcctcacaa gtaagatggc aggcgtttgt aatggctagg taaaattgta
     6541 accgtaaatc cctgagaaca cagggtttta tgggcaggag ttgctgaaag taaataaaaa
     6601 agtcgcagtg gatatcactg cgacttcctc tttggtcgcg gggacaggat tcgaacctgc
     6661 gacctccggg ttatgagccc gacgagctgc ct
//
LOCUS       DAOMQG010000170         6651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4949_length_6651_cov_10.634475, whole genome shotgun sequence.
ACCESSION   DAOMQG010000170 DAOMQG010000000
VERSION     DAOMQG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_6651_cov_10.634475"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLC06_10055"
     CDS             <1..200
                     /locus_tag="PLC06_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HRO90830.1"
                     /translation="GRVCMDQLMVNIEWETAYNGDEVILIGQTESGTTITVEELAEWA
                     GTIPWEILTNVNTRVPRVYKR"
     gene            356..772
                     /locus_tag="PLC06_10060"
     CDS             356..772
                     /locus_tag="PLC06_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRO90831.1"
                     /translation="MDAIIKLQDQFHFTAVAGNGYVVNLDTKAPEGEVSRGATPMELL
                     MISLAGCTAMDVVSILRKKRQELTGFEIHVHGERSDDHPKVFTDFELEYVVRGVNIDP
                     AAVERAIQLSVDSYCSVHAMLEKSAQIKTRYTIIQE"
     gene            803..1261
                     /locus_tag="PLC06_10065"
     CDS             803..1261
                     /locus_tag="PLC06_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90832.1"
                     /translation="MDLIHFLETEGDQIIDEAAEALNRSNLSRYNAAGPNVSRERLAE
                     LFRLMLIGIRERNLTPIIEYMNQVARERYHAGYAIREVQIAINVLEEAIWTHVVRVVP
                     PDQLAEALGLVSTALGAAKDTLARTYVTLAGESKAPSLDLSALFEGKTSG"
     gene            complement(1267..1989)
                     /locus_tag="PLC06_10070"
     CDS             complement(1267..1989)
                     /locus_tag="PLC06_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90833.1"
                     /translation="MKQSNYFAYGLLAIATDALTLIAALFILPQLITRMETPGGWNAL
                     LVSGAFLLFIIGTFMIRRMMPTAAGTAEWLTRGQRSVLALIFAFFFSLALAWQLGFFR
                     SAFLVDTTEMGEGGSSSYFVYGPGAWLAFSLAYVLVFAFRVEPRIESGSRGYWTAAVF
                     GLATTAVMTLALAAHGQAIAQQFGGGWLWSLITFAVLILLFLPARLLYLSRTTGLQTT
                     PGYLATAGLVILLGVLAMMPAG"
     gene            2278..3318
                     /locus_tag="PLC06_10075"
     CDS             2278..3318
                     /locus_tag="PLC06_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRO90834.1"
                     /translation="MNEDQGNDVRAGQSADDGYWASLFNTEDAFARDAGSASADVPPP
                     YDWLESPAQPASEDSRSTPLPSAVPAELPLNADPWAFAQRCMDADEILELRVADYNKG
                     GLLVFWNGIQGFVPASQLIDFPQFHIPRERHQTLAEWHNRVLRVKIIEVNQSNSRLIF
                     SERATLVAADERIDLLKGVHRGDRLKGTITNLTDFGAFVDLGGVEGLVHISEISWSRV
                     VHPSALLRQGQQVDVLVLSVDQKAERVALSIKQLHSDPWATAEERYRPGMRVSGVIGN
                     VTTYGAFVVLEEELEGLIHISELAEGAFMHPRDVVTTGDRVVARVLNVDARNKRIALS
                     LRGVGGDPTTSR"
     gene            3325..5556
                     /locus_tag="PLC06_10080"
     CDS             3325..5556
                     /locus_tag="PLC06_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRO90835.1"
                     /translation="MARSQPPAEKPPRKGNTRKPGNPPEETGPVSWDERLADEILNRR
                     REIGGVFFLLFAGVSLLALFDLSQASLLQWWTRLLRQIFGWGAIVICLAMAVLGLRLI
                     AGTLWGRIQVEPTRVAGAGILVIIAMGLAHLVGGYSLVDAVAGRGGGLVGWALAEPLF
                     DFFGPFLTILIYLALLGWAMMLLTGRTLADIQSGLMTASRRLSSWADQVDPDRVDVVG
                     RSEYHPPPATVAPPSAPALQLPPDEADGFSDTRRRDPRLPSIDLLERGETLTQARDEI
                     AWKSKRIEDTLFDFGLPAKVVEVRRGPTVTQFGVEPGYVERPGPDGEMKQQKVRVGQI
                     AALQRDLTLALAVSRLRIEAPVPGRNVVGIEVPNGQASVVRLRGLIESPAFARLNSPL
                     SVGLGRNVAGDPVVADLGKLPHLLIAGTTGSGKSVCINAFISCLVFNNTPEQLKLVMV
                     DPKKVELVRFNGLPHLIGNVEVDADRAVGVLRWLTSEMDRRYELFESVSARNISIYNH
                     KHSAAAKAHLRLPYIAVFIDELADLMHMYPGDVERNLCRLAQMARATGIHLIVATQRP
                     STDVITGLIKANFPARLSFAVASGTDSRVILDTVGAEHLLGKGDMLFQSPDAGAPDRV
                     QGVYVTDTEIERVVAHWRAATPNHAPEPPPWEPLIARYALLDETDSLLESAVELAKKH
                     DNLSVSFLQRRLRLGYPRAARLMEHLHEMGLVDDPQTGGKTRRSFVNEDDEDPIGDYL
                     SDS"
     gene            5569..6540
                     /locus_tag="PLC06_10085"
     CDS             5569..6540
                     /locus_tag="PLC06_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRO90836.1"
                     /translation="MSTIPKSQANFARYAWFVLTFLILVILWGAFVRATGSGAGCGSH
                     WPLCNGVVVPREPRIETLIELTHRVTSALSGVLVLAMLFWAFRLFPKGHIVRKAAAWS
                     TVFIITEGLVGAGLVLFEWVAHNQSVGRAISMAVHLVNTFLLLAAVALTAWWAGAGSD
                     YRLRLRGHGRVGWLIGGGVALLLVLGVSGAITALGDTLFPSGTLSEGIAADFEATAHF
                     LVRLRLYHPVIAVFTGLYLWLAAPIIARERPGIGPLFFSNAVRILVIVQLAAGVVNIL
                     LNVPVWMQLVHLLLADLMWIAVVLLGASALAEPVTAAQPATPALEPQ"
BASE COUNT         1273 a         1952 c         2017 g         1409 t
ORIGIN      
        1 tcggccgcgt gtgcatggat cagctcatgg tcaacatcga gtgggagacg gcctataatg
       61 gcgatgaggt gatcctgatc ggtcagacag agagcggcac gacgatcacc gttgaggagc
      121 tggctgagtg ggccggcacg atcccgtggg agattctgac caacgtcaac acgcgcgtgc
      181 cgcgagttta taagcgctga tccacagagc ctgcgaatga cgggtccaat tgtggggtct
      241 tcggattctc tttggccttg acacccttcg cggaatatgg gtaaattttc tcaataacac
      301 tgcgtctgat agaggagcaa cactctaaat tcttgattgg agccgaataa ttcaaatgga
      361 tgctattata aaacttcagg accaatttca ctttacggcc gtggccggca acggttatgt
      421 tgtcaatctg gatacgaaag cacccgaagg cgaagtcagc cggggtgcta ccccaatgga
      481 gctgctgatg atcagcttgg ccggttgcac ggccatggat gtcgtctcga tcttgcgcaa
      541 gaagcgccag gagctaaccg gcttcgagat tcacgtacat ggcgaacgtt ctgacgatca
      601 ccccaaggtg tttaccgact tcgagttgga atatgtcgtt cgtggcgtga acatcgaccc
      661 ggcggccgtg gaacgggcca ttcaactctc ggtcgatagt tactgctcgg tccacgccat
      721 gctggagaaa tcagcccaaa tcaagacccg ctatacgatt attcaggaat aggctttgcc
      781 tggagccatc gtggaggttg ttgtggattt gattcatttc ctggaaacag aaggcgatca
      841 aattatcgat gaggctgccg aagcgctgaa ccgttcgaac ctgtctcgtt acaatgcggc
      901 cgggcctaat gtcagtcgcg agcggttagc cgagttattc cggcttatgc tgatagggat
      961 tcgcgagcgt aacctgacgc cgataatcga atatatgaat caggtggccc gcgagcgcta
     1021 tcatgccggc tatgccatcc gcgaggtcca gattgccatc aatgtcctcg aagaggctat
     1081 ctggactcat gtggtgcgcg tcgtgccccc ggaccaattg gccgaggcat tgggcctcgt
     1141 cagcacggcc ctgggcgcgg ccaaggacac tctggcccgt acctatgtca ccttggccgg
     1201 cgaaagtaag gccccctctc tcgatctgtc ggctttattt gaaggaaaga ccagcggcta
     1261 acagatttaa cccgccggca tcattgccag gacccccaac agaatcacca gcccggctgt
     1321 cgccagatag ccgggcgtcg tttgcaatcc cgtcgtgcgc gatagataga gcaggcgcgc
     1381 cggcagaaag agcagaatca gcacggcaaa tgtgattaga ctccacaacc aaccgccgcc
     1441 gaactgctgc gcgatcgcct gaccatgagc cgcaagggca agggtcatta ctgccgtggt
     1501 cgccaggccg aaaacggccg cggtccaata tccgcgactc cccgactcga tgcgtggttc
     1561 tactctgaag gcgaaaacca gcacataagc cagggagaag gccagccacg cgcccggccc
     1621 ataaacgaag taagacgatg atccgccttc gcccatctcc gttgtgtcca ccaggaaagc
     1681 ggacctaaaa aaccccaatt gccacgccag cgccagacta aagaaaaaag cgaagattag
     1741 cgccagaacc gagcgttggc cgcgggtcag ccattcggcc gtgccggctg ccgtcggcat
     1801 catgcgccgg atcatgaaag tgccgatgat aaacagaagg aacgcgccgc tcaccagcag
     1861 agcattccac ccgcctggag tttccattcg ggtgatcagt tgaggaagga tgaacagtgc
     1921 ggcgattaac gtgagggcat cggtcgctat cgccaatagc ccataagcga aataattgga
     1981 ttgcttcatg tagaaggatt atatcaggcc gggttggcca cgtaacccat taacgccgtt
     2041 tcttctcgct caaatgcaat tatgtcaagc ttgtttgagt cgcgatccat cggttgacag
     2101 gccaatgact gaccctataa ttgattatcg ggttctgcat gaaaaggccg ttcgaacgta
     2161 tcgttttcca cgaaattggc cgccggtaat cagtaattgg cgtctacagg aacggcatca
     2221 ggggcacata acgggttggg aagtccgtgc tgtgtccact atgtaatggt tacagctatg
     2281 aatgaggatc aggggaacga tgttcgcgcc gggcagtcgg ccgatgatgg ctattgggca
     2341 tctttgttca acacggaaga cgccttcgcc cgcgatgcgg ggtcggcttc tgccgacgtt
     2401 ccgccgcctt acgattggct cgaatctccg gcacagcctg cctctgaaga cagccgctcc
     2461 acccccctgc cgtccgctgt ccctgccgaa ctaccactta acgctgatcc ctgggcgttc
     2521 gctcagcgat gcatggacgc ggatgagata ttggagttac gggtcgcgga ctacaataag
     2581 ggagggctat tggttttctg gaatggaatt caaggctttg tccccgcttc gcagctgatt
     2641 gattttccgc aatttcacat tccacgcgaa cgtcaccaga cactggctga atggcacaat
     2701 cgggtcctgc gagtcaagat catcgaggtc aatcagtcca acagccgcct catcttctcc
     2761 gagagggcga ccctggtggc ggccgatgaa cgtatcgatc tgctcaaggg cgtgcatcga
     2821 ggcgaccgac tcaaaggaac catcaccaat ctgactgatt tcggcgcttt cgtcgatctg
     2881 ggtggggtag agggcctggt ccacatctcc gagatatcct ggagccgggt cgtgcacccc
     2941 agcgcgttgc tacgccaggg gcagcaggtg gacgtgctgg ttctgagcgt cgatcagaag
     3001 gctgaacggg tggcgctgag catcaagcag ctgcattccg acccatgggc cacggccgag
     3061 gagcgctacc ggcccggaat gcgcgtgtcg ggcgtcattg gcaacgtcac cacctatggc
     3121 gcgtttgtcg tactggagga ggagcttgag gggctgattc atatctccga gcttgcggaa
     3181 ggcgccttta tgcatccgcg cgatgtcgtc acaaccggcg accgggtggt ggctcgtgtc
     3241 ctcaacgtcg acgcccggaa caagcgaatc gcgctctcgc tgcgcggagt gggcggcgac
     3301 cccacgacca gtcgctagga ttttatggcg cgctcgcagc ccccggccga aaagccgccc
     3361 aggaagggca acacccggaa gcctggcaac ccgccggaag agaccggtcc cgtctcgtgg
     3421 gatgaacggc tggccgatga aatcctcaac cggcggcgcg agattggcgg ggtattcttc
     3481 ttgctctttg cgggtgtctc tttattggcc ttgtttgacc tctcccaggc gagccttttg
     3541 caatggtgga caagactttt gcgtcagatt tttggttggg gcgcaatcgt tatttgtctg
     3601 gcaatggcgg tgctgggcct gcgcctgatc gccggcacgc tgtgggggcg catccaggtt
     3661 gagccaacca gggtggccgg agcgggcatc ctggtcatca tcgccatggg gctggcccac
     3721 ctggttggtg gctatagcct ggtcgatgcc gtggccggcc gcggcggcgg actggtgggc
     3781 tgggcgctgg ccgagccgct gttcgacttt ttcggcccat tcctgacgat cctcatctat
     3841 cttgcgctcc tcggttgggc tatgatgctg ctgaccggtc gaaccctggc cgacatccag
     3901 agcggattga tgactgcatc gcggcggctc tcaagctggg ctgaccaggt cgatccggat
     3961 cgcgtcgacg tggttgggcg cagcgagtat catccgccac cggcaaccgt tgccccaccg
     4021 tctgctcccg cgctccaatt gccgccggat gaggctgatg gcttcagcga tacgcgccgc
     4081 cgtgaccccc gcctgccgtc tatcgatctg ctggagcgcg gcgagacttt aacccaggct
     4141 cgcgacgaga tcgcctggaa gagcaagcgc atcgaggaca cacttttcga tttcggcctg
     4201 ccggccaaag tcgttgaggt gcgtcgcggc ccgacagtca cacagtttgg cgtcgagccg
     4261 ggctatgtcg agcgtcccgg ccctgacggc gagatgaagc agcagaaggt gcgcgtcggg
     4321 cagatcgccg ccctgcaacg tgacctgacg ttggctctgg ccgtgtcgcg cttgcgcatc
     4381 gaggcgcccg tgcccggccg caatgtggtc ggtatcgagg tgcccaatgg gcaggcgagc
     4441 gttgtccgac tgcgcggcct gattgaatcg cccgctttcg ctcggctcaa ttcgccgctc
     4501 agcgtcggcc tggggcgcaa tgtggccggc gaccctgtgg tggccgattt gggcaaactg
     4561 ccgcacttgc tcattgccgg gacgaccggc tccggcaagt cggtttgcat caatgccttt
     4621 atcagttgtc tcgtctttaa caatacgcct gagcagctga aactggtgat ggtcgacccg
     4681 aagaaggtgg agcttgtccg gttcaacggc ctgccccatc tgatcggcaa tgtcgaggtg
     4741 gacgccgacc gggccgtggg ggtattgcgc tggctgactt cagagatgga ccgccgctac
     4801 gagctgttcg agagcgtgag cgcgcgcaat atcagcatct acaatcacaa acattcggcc
     4861 gccgccaagg cgcatttgcg gctgccctat atcgccgtct tcatcgacga actggccgac
     4921 ctgatgcaca tgtaccccgg cgatgtggag cgcaatctgt gccggctggc gcaaatggcc
     4981 cgcgctacgg gcattcatct gattgtggcg acgcagcgcc cctcgaccga cgtgatcacc
     5041 ggcctgatca aggccaactt cccggcccgg ctctcgtttg ccgtggcgtc gggaaccgat
     5101 tcgcgcgtca tcctcgatac ggtgggagcc gagcatctgt tgggcaaggg cgacatgctt
     5161 ttccagtcac ccgacgccgg cgcacccgat cgggtgcagg gcgtctatgt gaccgatacg
     5221 gagatcgagc gcgttgtggc ccactggcgg gcggctaccc ccaatcacgc gcccgaaccg
     5281 ccgccatggg aaccgcttat cgcccgctac gcactgctcg acgagaccga cagcttgctg
     5341 gaatcggccg ttgagttggc caaaaaacat gataacctct ctgtgtcgtt tctccaacgc
     5401 cgcttgcgtc tgggttatcc ccgcgccgcg cggctcatgg aacacctgca cgagatgggg
     5461 ctggtggacg atccccaaac gggcggcaag acacgccgtt ctttcgtcaa cgaagatgac
     5521 gaagacccca tcggtgatta tctctccgat tcataatatt caaccgtcat gtcgactatc
     5581 cctaaatctc aagccaattt cgcgcgctat gcgtggttcg ttctgacatt tctgatcctg
     5641 gtcatactct ggggggcatt tgttcgtgcc accggttccg gagcgggctg tggcagccat
     5701 tggccgttgt gcaatggtgt ggtcgtgccc cgcgagccac gaatcgaaac gctcatcgag
     5761 cttacccatc gtgtcaccag cgctctttcc ggcgtcctgg tgttggccat gttgttttgg
     5821 gcattccggc tcttcccgaa aggccatatc gtgcgcaagg cggccgcctg gtcgacggtc
     5881 ttcatcatca ccgaagggct ggtgggggcg gggttggtgc tgttcgagtg ggtggcccac
     5941 aatcaatcag tgggccgcgc catctcgatg gccgttcatc tggtgaatac ctttctcttg
     6001 ctggcggccg tggccctaac tgcctggtgg gccggagccg ggagcgatta tcgccttcgg
     6061 ctgcgcggac atggacgtgt cggctggctg atcggcggcg gggttgcctt gttgctcgtc
     6121 cttggcgtca gcggggcgat cacagctctg ggcgacacct tgttcccatc cggcacattg
     6181 agcgagggga tcgctgccga tttcgaggcg acggcccatt tcctcgtccg gctgcgcctc
     6241 taccatcctg tcatcgccgt tttcaccggt ctctatctgt ggttggccgc gccgattatc
     6301 gctcgggagc ggccgggcat cgggccgcta ttcttttcca acgccgttcg gattctcgtc
     6361 atcgtccagc tcgctgccgg cgtcgtcaat attctgctga atgtgccggt ctggatgcag
     6421 ctcgtccatt tgctgttggc cgacctgatg tggatcgctg tagtgctgtt gggggcgtcg
     6481 gcgctggcgg aacctgtgac ggccgcgcaa ccggctactc cggctctcga accccaataa
     6541 aaaaagcccg ccgccggtaa accgggagcg ggcaattcaa tcaacggtaa cgatcaatca
     6601 ggcagcgccc gtataggtgc cgccggcctt gttgccgagg atcttggccg c
//
LOCUS       DAOMQG010000171         6632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4966_length_6632_cov_6.384370, whole genome shotgun sequence.
ACCESSION   DAOMQG010000171 DAOMQG010000000
VERSION     DAOMQG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_6632_cov_6.384370"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>1760
                     /locus_tag="PLC06_10090"
     CDS             192..>1760
                     /locus_tag="PLC06_10090"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRO90837.1"
                     /translation="MSEKQLTLPVIGMTCANCVAAVERNAKKVDGVTDAAVNFASEKV
                     TVTYDPAVSGTAAVTHDVIDRIRRAGYDVPTAEIELPLLGMTCTNCANTIERTLNKVD
                     GVVEATVNYANERATVRYAPGATTRADLVAAVRKAGYDVVEAAADADDMVDAEAAARE
                     AEIDHQKKRLLVGIIFTLPLFIISMGRDLNLIGSWAHALWVDWLFLALATPVQFYVGW
                     DYYTGAFKSLRNGSANMDVLVALGSSVAYFYSVAVLLAKSAGSMALGHHLYFETSAVI
                     ITLIVAGKLLEARAKGRTSEAIKKLIGLQPKTARVLRGSVEIDIPVAEVVVGDLLRVR
                     PGEKIPVDGRVTDGRSSVDESMITGESLPVDKKTGDAVIGATINRQGLLTVEAGNVGK
                     DTVLSQIIRMVEQAQGSKAPIQRVVDRVAAWFVPAVIIIALLTFAVWYIAGAGFVPAL
                     LRLIAVLVIACPCAMGLATPTSIMVGVGKGAEYGILFKNSAALEEAQRLGAVVLDKTG
                     TITRGQPAVTDVITN"
     assembly_gap    1761..1860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1861..2872
                     /locus_tag="PLC06_10095"
     CDS             <1861..2872
                     /locus_tag="PLC06_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRO90838.1"
                     /translation="TDPLTTDPLTTDPLTTDPLTTDPLNTDPLNTDPLTPDYLLRIAA
                     AAERGSEHPLGEAIVREAEMRGLDAPQPEAFEAIAGHGIAATVDGRRVLVGNRRLMER
                     EEVRLNGLAPRAEALEAAARTTMWVAADGEALGLIGVADTIKEGSAEAVRALHGLGLQ
                     VIMMTGDNQATAEAIAKEVGAAPGSAQIDRVFAGVLPGDKASYVKQLQGEGLRVGMVG
                     DGINDAPALAQADVGLAIGTGTDVAMETADVTLMRGDLRSVPQAIHLSKATMANIRQN
                     LFWAFGYNVVLIPIAAGVLAPFDWAPDFLRQLSPILAAGAMAFSSISVVTNALRLRGV
                     KL"
     gene            3012..3413
                     /locus_tag="PLC06_10100"
     CDS             3012..3413
                     /locus_tag="PLC06_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90839.1"
                     /translation="MTYRIDESLNPIIVVHFDEPLTVETATRLYREVDELFTRTGRFG
                     LIMRVGFTEGEAERGVTKMQKTWLSANRPRFKRDCAGIAMVSDDAKYVSLIAPLANKI
                     VSRMYHCPGAIFNDHDKALAWVRGKMASDDR"
     gene            3873..5906
                     /locus_tag="PLC06_10105"
     CDS             3873..5906
                     /locus_tag="PLC06_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90840.1"
                     /translation="MRTLKLSLHRGRQRFLPVIVPALFLVLALRPGPAAALGEAPPGL
                     PPQIIDVGYSHSCALTPTGAADCWTEAGAYGEGADKPGPYTQISTGPGLTCALTPAGA
                     ADCWGYGLDGRADDQPGPYIQIDAGHSHTCALTPSGAADCWGLNDVGQATDQPGPYTQ
                     ISAGADHNCGLKPDGSADCWGLNDVGQATDQPGPYTQISAGYYHTCALTPSGAADCWG
                     NNADGESDDQPGPYTQINAGYFYTCALTPAGAVDCWGLNDYGQADDQTGPYTQVSATN
                     RHTCALTPAGAADCWGLNGDGQADDQPGPYRPWEANEPNDTSGTATFLQYEETVRNNF
                     IGVAGDVDRYYFYAFANEQFVVEIEAASLGSDLDSVLTFYDSDGVTVLDESDDYQGGH
                     DSRLVVTIPATGYYGLGVRDVAHPDDGGPSSFYNLRIYPYSLYVSATKSGTVGSVAAT
                     PQDILRFDKNFLEWSMWFDGSDVGITQAVSSFARLPGGDWLLVFKANQPTPAGTFTPW
                     DIARFTPSSMGDTTAGTFSWFFDGSDVGLTAGGEKIDALDWVLNRILLSTGGTLSVPA
                     PGGTLKAQDEDLVAFYPTTTGANTAGEFAQWFDGTAVPGMKAEDLAAAHYDSTTGVLM
                     GAITGSFKIGSVSGSGKDILILTPDASPGGYSAAKLTLPPLGFNLLVAGFESE"
     gene            complement(6033..>6632)
                     /locus_tag="PLC06_10110"
     CDS             complement(6033..>6632)
                     /locus_tag="PLC06_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90841.1"
                     /translation="ADDVLISFTQNRRYVPGIAAPVNGMDLVWWDGSTFSLYFDGEDV
                     GLNQMTQEKIDSLHVLPGSESPIGGSCLSYLLLSTQGPGRVTAYDSTSLRFGGEDVLG
                     FCLTNAGSNTTGFWHMVLDGSAEGMPRNATDSISMSADGDTMYLTTRRPFNVDSASGG
                     HSMVYAYDFGTGTFSGPVFDAPANGLPRMVDGLNIVDLP"
BASE COUNT         1153 a         2195 c         2072 g         1112 t
ORIGIN      
        1 actgggagaa gatccgcgcc accctggtgg agatcaacta cccgcccgaa ggcctgatca
       61 cgatcaacta acgggagaag gggagagagg gagaaaggga gcaggggaga gagggagacc
      121 ccttcccccc tcccctcttc accctttcat cctttcaccc ttcctcccct cctccccctg
      181 agaaataaag catgagtgag aaacaactga cgctccccgt catcggcatg acctgcgcca
      241 actgcgtggc cgcggtagag cgcaatgcca aaaaggtcga tggcgtcacc gacgcggccg
      301 ttaatttcgc cagcgagaag gtgaccgtca cctatgaccc ggcggtcagc ggcacggcgg
      361 ccgtcaccca cgacgtcatc gaccgcatcc gccgcgccgg ctacgacgtg cccaccgccg
      421 agatcgagct tccgctgctc ggcatgacct gcaccaactg cgccaacacc atcgagcgga
      481 cgctcaacaa ggtcgacggc gtggtcgagg ccacggtcaa ctacgccaac gagcgagcca
      541 ccgtgcgcta tgcccccggc gccaccacgc gcgccgacct cgtcgccgcc gtgcgcaagg
      601 cgggctacga cgtggtggaa gcggcggccg acgccgacga tatggtcgac gccgaagcgg
      661 ccgcgcgcga ggcggagatc gaccaccaga agaagcgtct gctcgtgggc atcatcttca
      721 ccctgccgct cttcatcatt agcatgggcc gcgacctgaa cctgatcggc agctgggccc
      781 acgcgctgtg ggtcgattgg ctgttcctgg cattggccac gccggtgcag ttctacgtcg
      841 gttgggacta ttacaccggc gcgttcaaga gcctgcgcaa cggcagcgcc aacatggacg
      901 tgctggtcgc cctcggctcg tcggtggcct acttctacag tgtggccgtg ctgctggcca
      961 agagcgcggg cagcatggcg ctgggccacc atctctattt cgagacgtcg gccgtcatca
     1021 tcaccctcat cgtcgccggc aagctgctgg aggcgcgggc caaggggcgc accagcgagg
     1081 cgatcaagaa gctcatcggc ctgcagccca agacggctcg cgtcctgcgc ggcagcgtgg
     1141 agatcgacat cccggtggcc gaagtggttg tgggcgacct gctgcgcgtg cggccgggcg
     1201 agaagatccc cgtcgacggc cgcgtcaccg acggccgctc ctcggtcgat gagagcatga
     1261 tcaccggcga gagcctgccc gtggacaaga agaccggcga cgccgtcatc ggcgcgacca
     1321 tcaatcgcca ggggctgctg actgtcgagg ccggcaatgt gggcaaggac accgtgctgt
     1381 cgcagatcat ccgcatggtg gagcaggccc agggcagcaa ggcccccatc cagcgcgtgg
     1441 tcgaccgcgt ggcggcgtgg tttgtgcctg cggtcatcat tatcgccctg ctgacgttcg
     1501 ccgtctggta tattgccgga gccgggtttg tgcccgcgct gctgcggctc atcgccgtgc
     1561 tggtcatcgc ctgcccctgc gccatggggc tggccacgcc gacgtcgatc atggtcggtg
     1621 tgggcaaggg cgcggagtat ggcatcctgt tcaagaacag cgccgccctc gaagaggccc
     1681 aacgcctcgg cgcggtcgtg ctggacaaga cgggcacgat cacccgcggg cagccggcgg
     1741 tgacggatgt aattacgaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 tactgatcca ctgactactg atccactgac tactgatcca ctgactactg atccactgac
     1921 tactgaccca ctgaatactg acccactgaa cactgatcca ctgacccctg actacttgct
     1981 ccgcatcgcg gccgcggccg agcgggggag cgagcatccg ctgggcgagg cgattgtgcg
     2041 cgaggcggag atgcgcgggc tggacgcgcc gcagccggaa gcgttcgagg ccatcgccgg
     2101 gcacggcatc gccgccacag tcgacggccg ccgcgtgctg gtgggcaacc ggcggctgat
     2161 ggagcgcgag gaagtgcggc tcaacgggct ggccccgcgg gctgaagcgc tggaagcggc
     2221 ggcgcgcacc acgatgtggg tggccgccga cggcgaggcg ctgggcctca tcggcgtggc
     2281 cgacaccatc aaggaagggt cggccgaagc ggtgcgggcg ctccacgggc tggggttgca
     2341 ggtgatcatg atgaccggcg acaaccaggc caccgccgaa gccattgcca aagaagtggg
     2401 agccgccccc gggtcggctc agatcgaccg cgtgtttgcc ggggtactgc cgggcgacaa
     2461 ggcgagttat gtcaagcagc tgcaaggcga aggactgcgg gtgggcatgg tcggcgacgg
     2521 catcaacgac gctcccgcgc tggcccaggc cgatgtgggg ctggccatcg gcaccggcac
     2581 cgacgtggcc atggagacgg ccgacgtcac cctgatgcgc ggcgacctgc gcagcgtgcc
     2641 ccaggccatc cacctgtcga aggcgacgat ggccaacatc cgccaaaacc tgttctgggc
     2701 tttcggctac aacgtcgtgc tcatccccat cgccgcgggc gtgctggccc cgttcgactg
     2761 ggcgcccgac ttcctgcgcc agctcagccc catcctcgcc gccggggcca tggccttcag
     2821 cagcatcagc gtggtgacga atgcgctgcg gctgcgcggg gtgaagctct agtttagtga
     2881 cgagttacga gtgacgagtg gtcgtcactc gtcactcgta actcctttaa ccgcgtttca
     2941 atttccactt aatcgctgtc attgaatcgg attccgcgtc gtgttatggt gatgcctgcg
     3001 aggtttcacc catgacctat cgaatcgatg aaagcctgaa ccccattatt gttgtccatt
     3061 ttgacgaacc gctgacggtg gagacggcga cccgcctcta tcgcgaggtc gatgagctgt
     3121 tcacccgcac cggccgcttt ggcctgatca tgcgggtggg tttcaccgaa ggcgaggcgg
     3181 agcggggggt cacgaagatg caaaagacgt ggctctcggc caatcggccg cgcttcaagc
     3241 gggactgcgc cggcatcgcc atggtcagtg acgacgccaa atacgtgtcg ctcatcgcgc
     3301 cgctggccaa caagatcgtc agccgcatgt accattgccc cggcgccatc ttcaacgacc
     3361 atgacaaggc gctggcgtgg gtgcggggaa agatggcgag cgacgaccgc tgacgcgact
     3421 cctgtcgcgc ggacgcgact cctgtcgcgc ggacgcgact cctgtcgcgc ggacgcgacc
     3481 cctgtcgcgc agacgcgact cctgtcgcgc ggacgcgacc cctgtcgcgc gactcctgtc
     3541 gcgcggcggc cgcaaaagcg cgcctctttt cgtatttcgt atttcgtatt tcgtatttcg
     3601 tatttcgtat ttcgtatttc gtatttcgta tttcctcatt cataatccat aattttgctt
     3661 cccgttgtgt cggctggatg acagacagcg gcatcaattt ttgtttatac tgcccccaat
     3721 cacttcacca cacgggttcg cccgtcgtga gcgaggtgcc ggtcagtcgg ggcggcgcgt
     3781 cttgcggccc cccttaataa ccatcgcccc ccaaccgggg cgagccaacg ccttgcggca
     3841 gatccgccgc gaggctcaat aaaggagtcg tcatgagaac gttgaagctt tcattgcacc
     3901 gcgggcgaca gaggttcttg ccggtcattg tgccggccct gttccttgtg ttggcgctgc
     3961 gtcccggccc ggcggccgcg cttggcgagg cgccccccgg cctgccgccg caaataatcg
     4021 acgtcggcta ttcccacagc tgtgccctga cccccaccgg cgcggccgac tgctggacgg
     4081 aggccggcgc ttacggcgag ggggcggaca agcccggccc ctatacccag atcagcaccg
     4141 gccccggcct cacatgcgcc ctgacccccg ccggcgcggc cgactgctgg ggttacggcc
     4201 tcgacggtcg agccgacgac cagcccggcc cttatattca gatcgacgcc ggccacagcc
     4261 acacctgcgc cttgacgccg tccggcgcgg ccgactgctg gggccttaac gacgtcggcc
     4321 aggccaccga ccagcccggc ccctacaccc agatcagcgc cggcgccgac cacaactgcg
     4381 gcctgaagcc ggacggttcg gccgactgct ggggccttaa cgacgtcggc caggccaccg
     4441 accagcccgg cccctacacc cagatcagcg ccggctatta ccacacctgc gccctgacgc
     4501 cgtccggcgc ggccgactgc tggggcaaca acgccgacgg cgagtccgac gaccagcccg
     4561 gcccctacac ccagatcaac gccggttact tctacacctg cgcgctgacc ccggccggcg
     4621 cggtcgattg ctggggcctt aacgattacg gccaggccga tgaccaaacc ggtccctaca
     4681 cccaggtcag cgccaccaat cgtcatacct gcgccctgac cccggccggc gcggccgact
     4741 gctggggtct aaacggcgac ggccaggccg acgaccagcc cggcccctat cgcccctggg
     4801 aggcaaacga gccgaacgac acatcgggaa cggccacctt tctccaatat gaagagaccg
     4861 ttcgcaataa cttcatcggc gtggccggcg atgtggatcg ttactacttc tacgcctttg
     4921 ccaatgagca gttcgtggtc gagattgaag cggcttccct cggctccgac ctggattcgg
     4981 tgctgacatt ctacgacagt gacggcgtga ccgtcctgga cgagagcgac gactaccagg
     5041 gggggcatga ttcgcggctt gtcgtcacga ttcccgccac cggttattat ggcctgggcg
     5101 tccgcgatgt agcccacccg gacgatggcg ggccgagctc cttctataat ctgagaatct
     5161 acccatattc actctacgtc tccgccacca aaagcggcac ggtcggcagc gtggccgcca
     5221 ccccgcagga catcctgcgc tttgacaaaa acttcctcga atggtcgatg tggttcgacg
     5281 gctccgacgt gggcatcacc caggccgtgt catccttcgc ccggctgccc ggcggcgact
     5341 ggctgctggt cttcaaggcc aatcagccca ccccggccgg caccttcacc ccgtgggaca
     5401 tcgcccgctt cacccccagc tccatgggcg acaccaccgc cgggacgttc tcgtggttct
     5461 tcgacggctc cgacgtgggc ctgaccgccg gcggcgagaa gatcgacgcc ctcgattggg
     5521 tactcaacag gattctgctc agcaccggcg gcacactgtc cgtgcccgct cccggcggca
     5581 cgctcaaggc gcaggatgaa gacctggtcg ccttctaccc cacgacgacc ggcgcgaata
     5641 cggccggtga gtttgcccag tggtttgacg gaacggccgt gccgggcatg aaggccgagg
     5701 acctggccgc cgcgcattac gattccacca cgggcgtgct catgggcgca atcaccgggt
     5761 cgttcaagat cggcagcgtc agcggcagcg gcaaggacat cctcatcctg acacccgacg
     5821 cctcgccggg cggctacagc gccgccaagc tgaccctgcc gccgctcggc ttcaacctgc
     5881 tggtggccgg gtttgaatcg gaatagggga aagacgagtg acgagtgcct tacatagaaa
     5941 ccgggtttct cggaagaaac ccggtttcta aagatgtcgc agcgaaaccg ggtttcccgg
     6001 aagaaacccg gtttctctgt gtgtgcaagt cgttacggca gatcgacgat attcaggcca
     6061 tccaccatcc tgggcagtcc gttggccggg gcgtcgaaga cggggccgct gaaggtgccg
     6121 gtgccgaagt cgtaggcgta gaccatcgaa tggccgccgg acgcgctgtc gacgttgaac
     6181 ggccgccgcg tggtcaggta cattgtgtcg ccgtcggcgc tcatggagat gctgtcggtg
     6241 gcgttgcggg gcatgccctc ggcgctgccg tcgagcacca tgtgccagaa gccggtggtg
     6301 ttgctgccgg cgttggtcag acagaagccc agcacgtcct cgccgccgaa gcggagtgag
     6361 gtgctgtcat aggcggtcac gcggcccggc ccctgggtgc tgagcagcaa gtagctgagg
     6421 cagctgccgc caatgggcga ttcgctgccc ggcagcacat ggaggctgtc gatcttctcc
     6481 tgggtcatct gattcaagcc cacgtcctcg ccgtcgaagt acaggctgaa ggtcgaaccg
     6541 tcccaccaca ccagatccat gccgttgacc ggagcagcga tgccgggcac atagcggcgg
     6601 ttctgggtga aggagatgag cacgtcgtcg gc
//
LOCUS       DAOMQG010000172         6617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4974_length_6617_cov_7.569643, whole genome shotgun sequence.
ACCESSION   DAOMQG010000172 DAOMQG010000000
VERSION     DAOMQG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4974_length_6617_cov_7.569643"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..889
                     /locus_tag="PLC06_10115"
     CDS             92..889
                     /locus_tag="PLC06_10115"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO90842.1"
                     /translation="MKIVSKYPDGMFSWIDLGTTDLEAAHAFYSALFGWTPDNQPLPG
                     GGSYTNFRLDGYTVAGAAEMLPEMKANGVPPAWTSYISHSDIDAVAARAAEAGGTVFF
                     EPMDVMEYGRMAILGDPTGASFGLWQPKAHIGAQVVNQPNSFVWNELQTNDPAAARAF
                     YGKVFGWDERMDPSGYLMWADGDHVLCGCMTIDASWGDVPPNWLVYFLVDDVDAMAAR
                     VIELGGVVHHGPSDAGEMGRLAVVGDPQGAVFALIRFNGPADEPPGY"
     gene            complement(1076..1675)
                     /locus_tag="PLC06_10120"
     CDS             complement(1076..1675)
                     /locus_tag="PLC06_10120"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRO90843.1"
                     /translation="MKTPTDHSLAEQAALPCQACGRPVEAEVWIIVDVDARPDLLARL
                     RAGSLHDMTCSACGHTATLNTPLLVYRPGAEPALLFSPAAGSDADTDEAHAVALAGLF
                     REMMGELWRDEWLTHGLAGVARAALPVVLSDDPTTAAGLAVAHAAEGEVSPELRHVLE
                     VIMQSLAAEGVRVRTAEDLQRALESRPELKARLGAALNK"
     gene            complement(1848..6506)
                     /locus_tag="PLC06_10125"
     CDS             complement(1848..6506)
                     /locus_tag="PLC06_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRO90844.1"
                     /translation="MSRSPIPARRALALAAVLLLALLAAPALIVHAAAQDNNVQWTEL
                     GHNSRDTLFRSPGGAVPTGTAVRLRLRAADGDLTAAQVRVWDDRNDQQSLLPMTRVAS
                     GVTFTGDPTPYEFWEVTLPAPPDPTIFYYRFIAIDGSSTAYYEDDAARTGGWGQTFAN
                     SPDNSWQLTYYDPAFQTPDWVKNAIIYQIFPDRFRDGNSANNPIANQFFYGAFDTIVR
                     SNTTGWNTHICDPRNNVGSVTTCDLKYSQNFYGGDLQGIIDELDYLDGLGVTALYLNP
                     IFESPSNHKYDTKDFMVVDDNFGTLADFQTLVTEAGNRGIHIILDGVFNHSSSDSRYF
                     DRYSRWDAAGNPTTIGTNDGSGACESTASPYVDWYTFFNFVGTPPSPCSDNRDYPKWF
                     GIFDSLPVFQHDYPAVRDYFIGAGGGATSAVGPYWIDQGAAGWRLDVAPEIDHGQIND
                     PSDDYWENFREAVKSVDPDAYIVGEEWGNPTSWTVGGEWDATMNYQFAAAVLSFWRDE
                     PFSDNDFNSGSSAGVLNPLDATGVAERLLNLQERYAPEAFAAMMNLFNSHDTNRVLFL
                     LNHDADQNDVALYNNPNYDWSDSISRYEGALIMQMTLPGAPTIYYGDELGTVNPPGRD
                     GSTWQDDPYNRAPFPWLDETGTPFYAHMQNPAARTAMKDYVSLLTGLRNTRGELRTGS
                     FDVFDGGNANVFAYGRKSADGSQVAIVLVNKANTAQSVTVDLTGYVPFGAIFDDALSI
                     DNFTLDGADNLFAQLPAHGGRILLMTSTTSMPDAVDDLTAAAAGATQINLDWSAATGA
                     ASYDVYRSRLSGGGYEFVGNTTNTDYADTGLTTGVTYYYVVVSRASDGMASGWSNEDS
                     ATPFIDLSSSWRGLQWPPTLNHTASATLLTDNIYGQIWVGGVTDPPGATPGLLAQLGY
                     GDTTNTADGSWQWYPMTFNVQAGNNDEFMGNLPRTQIGTFCYTTRYSGDGGSTWYYAV
                     NGPNESNTTCPGPFGVLTVTTGSDTTAPAAPTTLAISGVTSASISLSWDAHPDTDGDL
                     YAFRVYRGPVGGPYSQIAQIVNPAATSYVDTTVTAGQTYEYYITAIDDSVNESAASNL
                     VSATAENLIVDVTFRVTVPSFTPDTVYLVGGFGGAGYPNWDPGAAAMAMTETSPNVWE
                     ITLQILDGTQLEYKYARGSWDKVEKQLDGFTEVDNRPFTADYGTTGTQLVEDTIANWR
                     DRIVVSHTPADGATGVDPSAVVTATWNKAMPATPTGTFSVTGPGGAVGGSLGWDGGTK
                     THTFTPSTPLAPGLYTVLIENNHDGGDNQFVSVTFSFTVPAPTEIFVTAAGGTVSGMP
                     YTKYDILRWDDNGWSKWFDGAAAGLNNRHDINAIAVPDGSTGEAWFTFSANRVMLPGM
                     VDWVMGQDVAYYDGAAWSLYIDGSDLGLTTIAEKIDSLEVIPNGAPGCEAVVLLSTQG
                     NGRVNGLGAIRGADIMTMCLIQSGANTTYSWIGIQRGLDLGMPNNATIALSMATADAT
                     ELFLLTRNVFNVDSASGGHSMVYRYDMAGESFDGPHWRATDHGLNQKTDGLDINGDID
                     "
BASE COUNT         1103 a         2052 c         2244 g         1218 t
ORIGIN      
        1 ctagaccaaa tgttctcttt tgtggtatga tgtgcccgtt ccaaaggcat ggaacgcaca
       61 tcgtcgaata ccatcaagga gaacaacacc catgaagatc gtttccaaat accccgacgg
      121 catgtttagc tggatcgatt tgggcaccac cgacctggag gcggcccacg ccttctattc
      181 cgcccttttc ggctggacgc ccgacaatca gcccttgccc ggcggcggct cctataccaa
      241 ctttcgcctc gacggctaca cggtggccgg cgcggccgag atgctgccgg agatgaaggc
      301 caacggcgtg cccccggcgt ggacgtccta catcagccac agcgatatcg acgctgtggc
      361 cgcccgcgcg gccgaggccg gcggcacggt ctttttcgag ccgatggacg tgatggaata
      421 cggccgcatg gccatcctgg gcgaccccac cggcgcctcg ttcggcctgt ggcagccgaa
      481 ggcccacatc ggcgcgcagg tggtcaatca gcccaactcg ttcgtctgga atgagctgca
      541 gaccaacgac ccggccgccg cccgcgcttt ctatggcaag gtcttcggct gggacgaaag
      601 aatggacccc agcggctatc tcatgtgggc cgacggcgac cacgtcctgt gcggctgcat
      661 gaccatcgat gcgagttggg gcgatgtgcc gcccaactgg ctggtctatt tcctggtcga
      721 tgatgtcgat gccatggcgg cgcgcgtgat cgagctgggc ggcgtggtgc atcacggccc
      781 gtccgacgcg ggcgagatgg ggcggttggc ggtcgtgggc gacccacaag gcgcggtatt
      841 cgcgctgatt cgcttcaacg gcccggccga cgagccgccg gggtattaga tctgcgacgc
      901 gaggcggggc gctagaactt gcgcccccgg gcgttccaga tgtagaacaa catcagcagc
      961 cccagaaaca ggggcacgag gacgaagagc atggggtaga ggtcggatga gcgcatgcat
     1021 ccatgatagc agatttcgca gaggaatgca gcttagccga tcggccgccc cattgctact
     1081 tgttcagcgc cgcgcccagc cgtgccttca gctccggccg tgattccagc gcgcgctgca
     1141 aatcttcggc cgtccggacg cgcacgccct cggcggccag cgattgcata atcacttcca
     1201 gcacatgacg cagttcgggc gacacctcgc cctcggccgc atgcgcgacg gccaacccgg
     1261 cagccgtggt cgggtcatcg ctcagcacca cgggcagcgc cgcgcgtgcc acgccggcca
     1321 gcccatgggt caaccattcg tcgcgccata actcgcccat catctcgcgg aacaagcccg
     1381 ccagcgccac ggcgtgggcc tcgtccgtgt cggcgtcact gccggcggcg ggggagaaga
     1441 gaagcgccgg ctccgcgccc ggccggtaga ccagcagcgg cgtgttgagc gtggccgtgt
     1501 ggccacaggc ggaacaagtc atgtcgtgga gcgagcctgc gcgcagccgc gccagcaggt
     1561 cgggccgcgc gtcgacatcg acgatgatcc agacctcagc ctcaaccggc cggccacatg
     1621 cctggcaagg cagcgccgcc tgttcggcca gagaatgatc ggttggcgtt ttcatggctc
     1681 cactttagcc cattgagcac aaaataacga gctacaagcc cggagcgcgc cgaagccacc
     1741 cctcgaactt gtagctcgtc gttcagtcgc tatcgctatc gcaatcggtg tcgttcttcg
     1801 attgcgacaa cgacaacgaa agcgatagcg acaacgatca cgatatccta gtcgatatca
     1861 ccattaatat ccaacccatc cgtcttctga ttcaacccat gatcggtggc gcgccaatgg
     1921 gggccatcga acgattcccc ggccatgtcg tagcgataga ccatcgaatg gccaccggaa
     1981 gccgaatcga cgttgaacac gttgcgcgtc aacaggaaca gctccgtcgc gtcggccgtg
     2041 gccatgctca gggcgatggt ggcgttgttg ggcatgccca ggtccagccc gcgttggatg
     2101 ccaatccagc tataggtcgt gttggcgccg gactggatca gacacatggt catgatgtcc
     2161 gcgccgcgaa tcgcgccgag gccgttgacg cggccgttcc cctgcgtgct cagcagcacc
     2221 accgcctcgc agccgggcgc gccgttgggg atcacttcca gcgaatcgat cttctcggct
     2281 atcgtcgtca ggcccagatc ggagccgtcg atgtagagcg accaggctgc gccgtcgtaa
     2341 taggcaacgt cctggcccat cacccagtcg accatgccgg gcaacatcac ccggttggcg
     2401 gagaaggtga accaggcctc gccggtcgaa ccgtcgggca cggcgatggc gttgatgtcg
     2461 tggcggttgt tcagccccgc ggccgcgcca tcgaaccatt tcgaccagcc gttgtcatcc
     2521 catctcagga tatcgtactt cgtgtaaggc atgccgctca ctgtgccgcc ggccgcggtg
     2581 acgaagatct ccgtcggcgc gggcaccgtg aagctgaagg tgacgctgac gaactggttg
     2641 tcgccgccgt cgtggttgtt ctcgatcagc accgtgtaca ggccgggagc cagcggggtc
     2701 gacggggtga aggtgtgggt cttcgtgccg ccgtcccagc ccaacgagcc gccgaccgcg
     2761 ccgccggggc cggtcacgct gaacgtgccg gtcggcgtgg cgggcatcgc cttgttccac
     2821 gtggccgtga ccaccgcgct ggggtcaacg ccggtcgcgc cgtcggccgg ggtgtggctg
     2881 accacgatcc ggtcgcgcca gttggcgatg gtgtcctcga ccagctgcgt gccggtcgtg
     2941 ccgtagtcgg cggtgaacgg ccggttatcg acctcggtga aaccgtcgag ctgcttctct
     3001 accttgtccc acgagccgcg ggcgtacttg tactccagct gcgtcccgtc gaggatctgc
     3061 agcgtgatct cccagacgtt gggcgaggtc tcggtcatgg ccatcgccgc cgcgccgggg
     3121 tcccagttgg gatagcccgc cccgccgaag ccgccgacca gatagaccgt gtccggcgtg
     3181 aaggacggca cggtgacgcg gaaggtcacg tcaacgatca ggttctcggc cgtcgccgac
     3241 accagattcg acgcggccga ctcgttgacg ctgtcgtcga tggccgtgat gtagtactca
     3301 tacgtctgcc cggcggtcac ggtcgtgtct acgtagctcg tggcggccgg attgacgatc
     3361 tgggcgatct ggctgtaggg gccgccgacc gggccgcgat agacgcggaa ggcgtagagg
     3421 tcgccgtcgg tgtcggggtg agcgtcccag ctcaggctga tgctggccga ggtgacgccg
     3481 ctgatcgcca gcgtcgtcgg cgcggccggg gcggtggtgt cgctgccggt cgtcacggtc
     3541 agcacgccga acgggccggg gcaggtggtg ttgctctcgt tggggccgtt gacggcatag
     3601 taccaggtgc tgccgccgtc gccgctgtag cgggtcgtgt agcagaacgt gccgatctgc
     3661 gtgcgcggca ggttgcccat gaattcgtcg ttgttgccgg cctggacgtt gaaggtcatg
     3721 gggtaccact gccagctgcc gtcggcggtg ttggtagtgt cgccatagcc gagctgggcc
     3781 agcaggccgg gcgtcgcccc gggtggatcg gtgacgccac cgacccaaat ctggccatat
     3841 atgttatccg tcagtaacgt ggcgctggcg gtatggttga gcgtcggcgg ccattgcaaa
     3901 ccgcgccagc tgctgctgag gtcgatgaag ggcgtggccg aatcctcgtt cgaccagccg
     3961 ctcgccatcc cgtcggacgc gcgcgagacg acgacgtagt agtaggtcac gcccgtcgtc
     4021 aggccggtgt cggcgtagtc ggtgttggtc gtgttgccca cgaattcata gccgccgccg
     4081 gaaagccggc tgcgatagac gtcatagctc gccgctccgg tcgccgcgct ccagtcgaga
     4141 ttgatctgcg tcgcaccggc ggccgccgcc gtcagatcgt ccaccgcgtc gggcatgctg
     4201 gtggtgctgg tcatcagcag gatgcggccg ccgtgggccg gcaattgggc aaacagattg
     4261 tcggcaccgt ccagcgtgaa attgtcgatg ctcagcgcat catcaaaaat ggctccgaag
     4321 gggacatagc cggtcagatc gaccgtgaca ctttgggcgg tgttcgcctt gttgaccagc
     4381 acaatggcga cctgcgaccc gtcggccgat ttgcggccat aggcgaacac gttggcgttg
     4441 ccgccgtcga acacgtcaaa cgagccggtg cgcagttcgc cgcgtgtgtt gcgcaggccg
     4501 gtcagcaggc tcacgtagtc cttcatcgcc gtgcgcgccg ccgggttctg catgtgggcg
     4561 tagaacggcg tgcccgtctc gtcgagccag gggaaaggcg cgcggttata cgggtcgtct
     4621 tgccaggtgg agccgtcacg gccgggcggg ttcaccgtgc ccaactcgtc accgtagtag
     4681 atggtcggcg cgccgggcag cgtcatctgc atgatcaatg ccccctcgta gcggctgatg
     4741 ctgtcgctcc agtcgtagtt gggattgttg tagagggcga cgtcgttctg gtcggcgtcg
     4801 tggttcagca agaacagcac gcggttggtg tcgtggctgt tgaacaggtt catcatcgcc
     4861 gcgaacgctt caggcgcgta acgctcttgc aaattgagca gtcgctcggc cacgccggtc
     4921 gcgtccagcg gattcagcac cccggccgaa ctgccgctgt tgaagtcgtt gtcgctgaat
     4981 ggctcatcgc gccagaagct gaggacggcc gcggcaaact ggtagttcat cgtcgcgtcc
     5041 cactcaccgc ctaccgtcca cgaggtgggg ttgccccatt cctcgccgac gatgtaggca
     5101 tcggggtcaa ccgacttcac cgcctcgcgg aagttctccc aatagtcgtc gctgggatcg
     5161 ttgatctggc catggtcgat ctccggggcc acatcgagcc gccagccggc cgcgccctgg
     5221 tcgatccagt aggggccgac ggcgctggtg gctccgccgc ccgcaccgat gaagtagtcg
     5281 cgcaccgccg ggtagtcgtg ctggaagacc ggcagcgagt cgaagatgcc gaaccacttg
     5341 gggtagtcgc ggttgtcgga gcagggggac ggcggtgtgc cgacgaagtt gaagaaggtg
     5401 taccagtcga cgtagggtga ggccgtggat tcgcacgcgc cgctgccgtc gttggtgccg
     5461 atggtcgtcg ggttgccggc ggcgtcccag cggctgtagc ggtcgaagta gcggctgtcg
     5521 gacgagctat ggttgaacac gccgtcgagg atgatgtgga tgccgcgatt gccggcctcg
     5581 gtcaccagcg tctggaaatc ggccaatgtg ccgaagttgt cgtcgaccac catgaaatcc
     5641 ttggtgtcgt acttgtgatt cgacggcgat tcgaagatcg ggttgaggta gagcgccgtg
     5701 acgcccagcc cgtcaaggta gtccagctcg tcgatgatgc cctgcaagtc gccgccatag
     5761 aagttctggc tgtacttgag gtcgcaggta gtcacacttc caacgttgtt tcgcggatca
     5821 cagatgtggg tattccagcc cgtcgtgttg ctgcgcacga tggtgtcgaa cgcgccatag
     5881 aagaactggt tggcgatggg gttattggcg ctgttgccgt cgcggaagcg gtcggggaag
     5941 atctggtaga tgatggcgtt cttcacccag tcgggggtct ggaaggccgg gtcgtagtag
     6001 gtcagctgcc agctgttgtc ggggctgttg gcgaaggtct gcccccagcc gccggtgcgg
     6061 gcggcgtcgt cttcatagta ggccgtggag ctgccgtcga tggcgatgaa gcgatagtag
     6121 aaaatggtcg ggtcgggcgg ggcgggcagc gtcacttccc agaactcgta gggggtcggg
     6181 tcgccggtga aggtgacgcc ggaggccacc cgcgtcatgg gcaggaggct ctgctggtcg
     6241 ttgcggtcgt cccagacgcg cacctgggcg gcggtcaggt cgccgtcggc ggcgcgcagc
     6301 cgcaagcgga cggccgtgcc ggtggggacg gccccgccgg gactgcggaa cagcgtgtcg
     6361 cggctgttgt ggcccagttc cgtccattgg acgttgttgt cctgggctgc cgcgtggacg
     6421 atgagcgccg gcgcggccaa cagggccagc aataaaacgg ccgccagcgc cagagcgcga
     6481 cgggctggaa taggggatcg tgacatggtt tctttctcca gactcgaaac gatagtggct
     6541 cccgctcggc cgaaccgccg gcagcctgtt gccaataaaa aagagcagcg caaaacagcg
     6601 ctgctccttc cttcagt
//
LOCUS       DAOMQG010000173         6594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4991_length_6594_cov_8.920936, whole genome shotgun sequence.
ACCESSION   DAOMQG010000173 DAOMQG010000000
VERSION     DAOMQG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4991_length_6594_cov_8.920936"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLC06_10130"
     CDS             complement(<1..908)
                     /locus_tag="PLC06_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRO90845.1"
                     /translation="MSYKILIADKLGEDGLDRLGQMSDMSYDLRTGMSKEELLQAVGE
                     YDAVIVRSDTYIDADVIAAGKRLKVVGRAGIGVDNIDVAAATAHGVRVMNTPHSNSVA
                     TAEQTFTLFLVTSRYTVQAHNSVAAGQWERSRFVGTELYAKTLGIIGFGYIGRLVARR
                     AQAFGMKVVAYDPYVKAETAAELDVELLPMNEVLAAADVITLHAIVTPETKGMINADT
                     IATMKDGVIIVNVARGKLIDEQALADALKSGKVAAAGIDVYQEEPPTNSPLIGLPNVV
                     HTPHLGASTQEAQKNVGIEMVEQIADG"
     gene            complement(1050..2501)
                     /locus_tag="PLC06_10135"
     CDS             complement(1050..2501)
                     /locus_tag="PLC06_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRO90846.1"
                     /translation="MADEYRIEKDSLGEVRVPAGAYWGAQTQRAIHNFPITGLRPYPA
                     FVWSMTLIKRAAAEVNSDLGLFHDKEAGGRTINGNDMAAAIIQAGDEILGGKFADQFV
                     VDPIQAGAGTSHNMNINEVMANRANEILGFGLGEKGKPVNPNDHVNMAQSTNDTIPTA
                     IRLGALWRLDELLATLDRLAAELNAKAREFDGIVKSGRTHLQDAVPVRLGQEFGAYAR
                     AVERDADKIRQAAEGLRRLGIGGTATGSGLNAHPEYHRRMVVVLSRLSGLELHESDNL
                     FESMQSMADAVHFSGSLRTLAQDLTRIANDFRLLASGPTTGLDEIRLPPVQPGSSIMP
                     GKVNPVMAEMLNMAMFQVMGNDLTVMLAGQAGQLELNVMMPIIAFNLFQTMDVIINSV
                     NAFIDFCLVDLTANAEKATGWLAKNAILVTALNPVIGYQKGAEVAKEALATNRTVREV
                     VIEKGYITPEEADKLLDVSALTEGGIQGVAAGG"
     gene            complement(2836..3039)
                     /locus_tag="PLC06_10140"
                     /pseudo
     CDS             complement(2836..3039)
                     /locus_tag="PLC06_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            complement(3112..3723)
                     /locus_tag="PLC06_10145"
     CDS             complement(3112..3723)
                     /locus_tag="PLC06_10145"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRO90847.1"
                     /translation="MSFELPPLPYAEDALEPYIDARTMSIHHDKHHAAYTNNLNAALK
                     DHGDLAGKSIEQLLGDLGALPESIRTAVRNNGGGYANHNLFWETMAPKHGGEPSGALA
                     AAIDQAFGSFAAFQEQFAKAAATRFGSGWAWLYVDKNGGLAVGSTPNQDTPLMEGNTP
                     LLGLDVWEHAYYLNYQNRRPDYVSAWWNVVNWDVVAAKYAAAK"
     gene            complement(3863..6391)
                     /locus_tag="PLC06_10150"
     CDS             complement(3863..6391)
                     /locus_tag="PLC06_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90848.1"
                     /translation="MDESPEKPIAAEDGRARKLLPVVALVVASLIVFGVATLGARGHC
                     VWSDLCGPIVAEDTAQGRLDTALPAPQGDFQIEQTFIPRRDGLSEVELLLVRYGEGAT
                     GAADPGTFSVTLHDETGALVAVEQLSAGELSHNQTYLLRFPPQEDSAGHRYTLRLSGN
                     EHNSVSAWGYSLDADAGGALRLSGRPPEGGTAAVDLRYVTRYTLTAGDTLAAALAPAR
                     EWLPLLTALLFLPLPGVWLLLLVRPRGWDRAAWWGVALSLGVAAWPVLWQWVSLAGGR
                     WSGAMLWWVVGGGWAAAVAVARWRGRSLAGREAQPAPSAPKGQSAILAYALLTILILA
                     ATASRFVAVRDLAFPPWVDSSRHALITAVMLDSGRIPTDYAPYLPVDHFPYHYGFHTL
                     SASLILMTGSALPGALLFLMQLLGGLLPLTVYAAGWLVTRRRVVGLWAAFLVALPFFF
                     PGYYATWGRMTQLSAMMVMPVLLALTWRAGRDWPRVWPLVGVSAAGVFLIHFRVFLFY
                     LPFAALVAVACLLARRSARGLVGAGLLGAILIAPRLIELLIVTEPLATVSRSLPGYND
                     FPMGYITTGWERAFLIAAVIAAAVVVGGVALRRRWATFPFLLLLWVGVLFTLLAGERL
                     GLPESLVVNLNSMYITLFLPQALLLAIVAGRIGAWTQRLLGNSPLGVVPAAAVGVVLG
                     ALALFGWRQQSNILNPQTILALPQDMAALQWIDDNLPADARVAVSSWRWLGETWAGSD
                     GGAWIVPLTGRESTTPPIDHIYNVELFADVRAFNEAAVAVTDWSAPEAADWLVAQGVT
                     HVFVGRRGGFLDPAALSRNPSLTLIYAHDGTFVFAAAAPDLVGQ"
     gene            complement(6413..>6594)
                     /locus_tag="PLC06_10155"
     CDS             complement(6413..>6594)
                     /locus_tag="PLC06_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90849.1"
                     /translation="LERRSGVVLQGEKEGGTKSFHDATCKEGSPSIHIVEINPYNQHY
                     ENDPFETSFQIWVRH"
BASE COUNT         1274 a         2112 c         2045 g         1163 t
ORIGIN      
        1 ccgtcggcga tctgctcgac catctcgatg cccacgttct tctgcgcttc ctgcgtgctc
       61 gccccaagat gaggggtgtg gacgacattg ggcaggccga tcagcgggct gttggtcggc
      121 ggctcttcct gatacacgtc gatgccggcg gcggccacct tgcccgactt caacgcatca
      181 gccagcgcct gctcgtcgat cagcttgccg cgagccacat tgacgatgat cacgccgtcc
      241 ttcatggtgg cgattgtgtc cgcgttgatc atccccttcg tctccggcgt aacgatggcg
      301 tggagcgtga tcacgtcggc cgcggccagc acctcgttca tgggcagcag ctcaacgtcc
      361 agctcggcgg ccgtttccgc cttcacatag gggtcatagg ccacgacctt catgccgaac
      421 gcctgggcgc gacgggcgac aaggcgaccg atgtagccga agccgatgat acccagcgtc
      481 ttggcataaa gttcggtgcc tacaaagcgg gagcgctccc attgcccggc ggcgacggag
      541 ttatgggcct gaacggtata gcgcgacgtg accaggaaca aggtgaaggt ctgctcggcc
      601 gtggccacgc tgttggaatg gggggtgttc atcaccctga cgccgtgggc ggtagccgcc
      661 gctacgtcga tgttatcgac gccaatgccg gcgcggccga cgaccttcag ccgcttcccg
      721 gcggcgatga cgtccgcgtc gatgtaggtg tcgctgcgca cgatgaccgc gtcgtattca
      781 ccgacggcct gcagcagctc ctccttgctc attccggtac ggaggtcata gctcatatcg
      841 ctcatttggc ccagccggtc cagcccgtcc tcgcccaatt tatccgcgat caggattttg
      901 taactcattg gttcctccag cagattatca gagaactgtt cgaagtacga aagacggagt
      961 acgtcacttc cagaaaccgg gtttctcgga agaaacccgg tttctacgct gcgctcttca
     1021 ttcctgattc gtaattccta attgatgaat catccccccg ccgcgacgcc ctggatgccg
     1081 ccctcggtca gggcgctcac gtcgagcagc ttgtccgcct cttcgggggt gatatagccc
     1141 ttctcgatga ccacctcgcg caccgtgcga ttggtcgcca gcgcttcctt ggccacctcg
     1201 gcccccttct ggtagccgat gaccggattg agcgcggtca cgagaatggc gttcttggcc
     1261 agccagccgg tcgccttctc ggcgttggcc gtcagatcga ccagacagaa gtcgataaag
     1321 gcgttgacgg agttgatgat cacgtccatc gtctggaaca ggttgaaggc gatgatgggc
     1381 atcatcacgt tcagttccag ctgaccggcc tgcccggcca acatcaccgt caggtcgttg
     1441 cccatcacct ggaacatggc catattgagc atctcggcca tgaccgggtt caccttgccg
     1501 ggcatgatcg acgagccggg ctgaaccggt ggcaggcgaa tctcgtccag ccccgtcgtg
     1561 gggccggaag ccagcaggcg aaagtcgttg gcgatgcggg tcaggtcctg ggccaacgtg
     1621 cgcagcgatc cgctgaaatg aacggcgtcg gccatgctct gcatcgactc gaacagattg
     1681 tcgctctcat gaagctccag gccgctcaac cggctgagga cgacgaccat gcgccgatga
     1741 tattcggggt gggcgttgag gccggagccg gtggctgtgc cgccgatgcc gagccggcgc
     1801 agcccctcgg ccgcctggcg aattttgtcg gcgtcacgct ccacggcacg ggcataggcg
     1861 ccgaactcct ggcccaggcg cacgggcacg gcgtcctgca aatgggtgcg gccgctcttg
     1921 acgatgccgt cgaactcgcg ggccttggcg ttcagctcgg ccgccaaccg atccagcgtc
     1981 gccagcagct catccagccg ccacaatgcg cccaggcgga tggccgtggg gatggtgtcg
     2041 ttggtggact gggccatgtt gacgtggtcg ttggggttga ccggcttgcc cttctcgccc
     2101 aaaccaaagc cgaggatctc gttggcccgg ttggccatga cctcgttgat gttcatgttg
     2161 tggctggtgc ccgcgccggc ctggatggga tcgacgacaa actggtcggc gaacttgccg
     2221 ccgaggatct cgtcgccggc ctgaatgatg gcggcggcca tatcgttgcc gttgatggtg
     2281 cggccgcccg cttccttatc gtggaaaagg cccaggtcgc tgttgacttc ggccgcggcc
     2341 cgcttgatca gggtcatcga ccagacaaac gccggatagg ggcgcaagcc ggtgatgggg
     2401 aagttgtgga tggcgcgctg ggtctgcgcg ccccaatacg caccggccgg aacgcgaacc
     2461 tcgcccagcg aatctttctc gatgcgatat tcatctgcca tggtgaattc ctccagaaaa
     2521 caataaaccg aattaaccaa ccggagttcc aggttagtta attcggttca ggtaccagaa
     2581 aatgatggta aggcggccga gccggagctt acaatcggcc cgaaggcatt acatattcaa
     2641 cgcgtcgtag ccgggtcctt cagagtagaa cgcgtagttg gccggaatat cctcggtcag
     2701 gtcgagtact tcgccgccgg ccatttgctt ggcgttgagc agcttgacgt ggtggccgtc
     2761 gatctcgccc tcgaacggca cgccgtccgg cagctcttcc ttcgtgccgc caatccagat
     2821 tcccgccgga gccgtgaagt cgaaggggac ctcctcttcg gggaagatgg cctcataggg
     2881 gcactcgggg acgcacgcgc cgcagtcgat gcacgtatcg ggatcgatgt agagcaaggg
     2941 ccacttgtcc tccggctggc ccaggaccat acattcgacc ggacatacct cgacgcaagc
     3001 tgtgtcacga aggcacagtc gggtaatgat atgggtcatt gactatctcc ttaaaagatc
     3061 gttgccgacc gggctgcctg ttgggccaca ccggcgcggt taaaagtgtt attatttcgc
     3121 ggccgcatat ttggccgcga cgacatccca attgacgaca ttccaccagg cggagacata
     3181 gtccggccgg cggttctggt agttcagata gtaggcgtgc tcccacacat ccagaccgag
     3241 gaggggggta ttgccttcca tgaggggcgt gtcctggttg ggcgtcgagc caaccgccag
     3301 cccgccgttc ttgtcgacat agagccaggc ccaaccggag ccgaagcggg tcgcagcggc
     3361 cttggcaaac tgctcctgga aagccgcaaa gctgccgaaa gcctggtcaa tcgccgcggc
     3421 cagcgcgccc gacggctcgc cgccgtgctt gggggccatc gtttcccaga acaggttgtg
     3481 attggcatag ccgccgccgt tgttgcgcac agccgtgcgg atggattcgg gcagcgcgcc
     3541 cagatcgccc agcagttgct caatgctctt gccggccaaa tcgccgtggt ccttcagggc
     3601 cgcgttcagg ttgttggtgt aggccgcgtg gtgtttgtcg tggtggatgc tcatggtgcg
     3661 agcatcgata tagggttcca aagcatcttc cgcataaggc agcggaggta attcgaaaga
     3721 cattactatc tccttggtta gtgtatgtca cccaccgttg agctaaaggg tgaacagcgg
     3781 gtcgtggctt cgattggatt ctagcgcagt attttcggat gtcaaacctg aatttaaccg
     3841 tggttaatag atttcacaag cgctattggc ccaccaaatc gggggcggcc gcggcaaaaa
     3901 cgaacgtccc gtcatgagca taaatcagcg tcaacgacgg gttgcgactc agtgctgccg
     3961 ggtccagaaa gccgccgcgc cggcccacaa acacatgagt gactccctgc gccaccagcc
     4021 agtcggccgc ctccggcgcg ctccagtcgg tgacggccac ggcggcctca ttgaaggcac
     4081 gcacatcggc gaacaactcg acgttataga tatgatcgat tggtggagtg gtcgattcgc
     4141 gcccggtcag gggcacgatc cacgccccgc cgtcgctccc ggcccaggtc tcccccagcc
     4201 agcgccatga gctgacggcc acgcgagcgt cggccggcag gttgtcgtcg atccattgca
     4261 gcgcggccat atcctgcggc agggccagga tggtttgcgg gttcaggatg ttgctctgtt
     4321 gtcgccagcc aaacaacgcc agcgcgccca gaaccacccc aaccgcagcc gcgggcacga
     4381 cccccagcgg cgagttcccc agcagacgtt gagtccatgc cccgattcgc ccggcaacga
     4441 tggccaggag cagcgcctgg ggcagaaaga gggtgatgta catgctgttg aggttcacca
     4501 ccagcgactc gggcagaccc agccgctcgc cggccagcaa ggtgaacaga acgccaaccc
     4561 atagcaacaa caggaacggg aaggtggccc aacgacgacg cagcgcgacc ccacctacga
     4621 caacggccgc ggcgatgacc gctgctatca gaaaggcgcg ttcccagccg gtggtgatgt
     4681 agcccatcgg gaaatcgttg tagccgggga gcgagcgcga aacggtggcc agcggttcgg
     4741 tgacgatcag caactcgatc agccgcgggg cgatcagaat cgcgcccaat agcccggccc
     4801 cgaccagccc ccgcgccgaa cggcgagcga ggaggcaggc cacggcaacc agcgcggcga
     4861 acggcagata aaacaggaac acgcggaaat ggatcaggaa caccccggcg gccgacaccc
     4921 ccaccagcgg ccacacacgc ggccaatcgc ggccggcccg ccaggtgagc gccagcaaca
     4981 ccggcatcac catcatcgcc gacaactggg tcatccggcc ccaggtggca taatagccgg
     5041 ggaagaagaa gggcagcgcc accaggaagg ccgcccatag cccaacgacc cgccgcctcg
     5101 ttaccagcca tccggcagcg tagaccgtca gcggcaacag cccgcccagc agctgcatca
     5161 gaaagagcag cgcccccggc agcgcgcttc ccgtcatcag gatcagcgac gccgacaggg
     5221 tgtggaagcc gtaatgatag gggaaatgat cgacgggcag ataaggcgcg taatcggtgg
     5281 ggattcggcc gctgtccagc atgacggctg tgatgagcgc gtgacggctg gaatccaccc
     5341 acggtgggaa ggccagatcg cgcacggcga cgaagcgcga ggcggttgcg gcgaggatca
     5401 ggatagtcag gagggcgtaa gccaggatcg ccgattgacc tttcggggcc gacggggccg
     5461 gttgcgcttc ccgaccggcg agcgatcgac cgcgccaccg ggcaaccgcc accgccgcgg
     5521 cccacccgcc gcccaccacc caccacaaca tcgcgcccga ccagcgaccg cccgccaacg
     5581 acacccattg ccacaggacg ggccacgccg ccacgccgag cgacagcgcc accccccacc
     5641 acgccgcccg atcccaaccg cgcggccgca cgagcagcaa cagccacacg cccggcaagg
     5701 gcagaaacag cagcgccgtc aacaagggca gccactctct cgctggggca agggcggcgg
     5761 cgagagtgtc gcccgcggtc agggtgtaac gggtgacgta gcgcaaatcg actgcggccg
     5821 ttcccccttc gggcgggcga cccgacagac ggagcgcacc gccggcgtcc gcgtcgagcg
     5881 aatagcccca ggcagagaca gagttgtgtt cattaccgga aaggcgcaat gtgtaacggt
     5941 ggccggccga atcctcttga ggcggaaagc gcagcagata ggtctggttg tgactcagct
     6001 cccctgcgga gagctgttcg acggccacaa gcgcgccggt ctcgtcatgg agcgtcacgg
     6061 agaaagtgcc cgggtcagcc gcgcccgtcg ccccttcgcc atagcgcacc aggagcagtt
     6121 ccacctcgct caggccgtcc cgccggggga tgaaggtttg ctcgatctgg aagtcgcctt
     6181 gcggggcggg cagggccgta tccaggcggc cctgggcggt atcttcggcc acaatcggcc
     6241 cgcacaggtc gctccacacg caatgccccc gcgcgccgag cgtggccacc ccgaatacaa
     6301 tcagcgaggc gacaacgagc gcgacgacgg gcaacagctt tcgcgcgcgg ccgtcctcgg
     6361 ccgcaatcgg cttctcgggc gattcatcca ttgccgatga ctatatcttc agttaatggc
     6421 gcacccaaat ctgaaaactc gtttcaaatg gatcattttc gtaatgttga ttgtaaggat
     6481 ttatttctac tatatgaatt gacggacttc cctccttgca agtggcgtcg tgaaaagatt
     6541 ttgtccctcc ttccttctcc ccctgaagca cgacgccact tcttctttct aacc
//
LOCUS       DAOMQG010000174         6588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_4997_length_6588_cov_6.517526, whole genome shotgun sequence.
ACCESSION   DAOMQG010000174 DAOMQG010000000
VERSION     DAOMQG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4997_length_6588_cov_6.517526"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1892
                     /locus_tag="PLC06_10160"
     CDS             63..1892
                     /locus_tag="PLC06_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRO90850.1"
                     /translation="MDSQQHDQNAEDRYRERSAERAAQQDNAAKGIVDFGEMTRRDAR
                     RDTLHPNDGLGLERILGNSDLLEINFLDIGRRAGRAVGRVEIRDTAGRIRGFGTGFVV
                     SPNLLLTNNHVLPTADSARRSLIDFDYEDDESYNPRSPVVFGLDPDRFYHTSAALDYT
                     VVAVGRVSVDSQTPLASFGYLPLSAYQGKILLDEYVAVIQHPGGAPKKIALRNNKLVD
                     LFDDFIHYTTDTDRGASGSPVFNDQWQVVGLHHAGVRKRDAQGRILAVDGSVWTPAMG
                     EDRIAYIANEGVRISRILADLRAAADGLSAEQQVLLDELLAEPATPPTTAGTVTPLRT
                     AERDPELFTRAKGYESGFLGPDVELPALSPEQLADVAPRLDGRGHVLDYVHFSLVMSK
                     ARRLAFYTAVNIDGRKTKTIKRDDDVWYFDPRIAREHQTGADLYERNDLDRGHLVRRN
                     DPVWGRPAATANEDTFHFTNCAPQHKQLNRRTWLALEDYILNNADNHDLKVSVFTGPV
                     FRADDMTYRGQYQLPAEFWKVVVMRKPDRKLSATAYLQTQKNLIEDLEFAYGAYRTYQ
                     VPVTRIEAITGLDFGPLRDHDPLAATESASPALVVGEAADIRL"
     gene            complement(1887..>3346)
                     /locus_tag="PLC06_10165"
     CDS             complement(1887..>3346)
                     /locus_tag="PLC06_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO90851.1"
                     /translation="MDRAEMAALPADEVDYSKKWHVMSAIAMGIFLATIDGSIVNVAL
                     PTLTVELNTDFSSVQWVVLSYLLTVTILQAIVGRLADMHGRKHLYNGGFVVFTIGSAL
                     CGLSPTVEWLIMARVLQGIGGAMTLALGMAIITDAFPAGERGRALGITGSIVSIGIVT
                     GPTLGGVIIEHLSWHWIFFVNVPIGIIGTWLAWRHIPHTKPHGDQVFDLAGAATLCVF
                     LLALLLGLTTGQLQGFTSSQALGLFIVAAFFLVTFIILESRHEQPIIPLSLFDNRVFR
                     VNIITGLMIFIAMGTYALIPFYLENALKYSVQQVGFLVAVVPLALGITAPISGSLSDR
                     VGSRPISVIGLVVSTFAFLLMSTLTAETTAIGFMLRFLPLGIGVGMFQSPNNSAIMGN
                     VPRERLGVASSLLAVTRSLGQTVGFAVLGSVWAGLVFRASGGILTGGAESGSPEAIAA
                     GIQGTALVSAGLAAVALVIALFAWRNERLTRRSSL"
     assembly_gap    3347..3446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3447..4060)
                     /locus_tag="PLC06_10170"
     CDS             complement(<3447..4060)
                     /locus_tag="PLC06_10170"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRO90852.1"
                     /translation="MHLYFIRHGQSFVNLKEWNGGNQDVGLTDLGHRQAAALGTWLPT
                     ELKEIDALYASTMVRAQETAGYVARAYNMDIIHDDRLREIGNNRADHVPWPSDDLPEY
                     SDYWGSERPFASITPDREGGESLMHFRVRVGSFIENMIELHRDQVVVAVCHGGVIELA
                     LDHIFNVGPFRRCEVWCKNTGVTRFEYVEHPRREMWRLHYHSRA"
     gene            complement(4270..4908)
                     /locus_tag="PLC06_10175"
     CDS             complement(4270..4908)
                     /locus_tag="PLC06_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRO90853.1"
                     /translation="MKIDIFSDTVCPWCRIGKRHLELALADWPGEPVTIRHRSFFLDP
                     DIPAEGYDFQAHMTAKGGGRMTPEDFFAMPRQRGKAVGLTFNFEDIAKAPNTMLSHRL
                     VYLTPEAERGAMLDAIFAAYFEFGRDIGDPDVLAAIAAEQGQDAAAIRAALAGDAAEA
                     QVLADIAFAQQAGISSVPFFIFNDKYALSGAQPVEVMGRVLRQVAEKDLSGS"
     gene            5055..5741
                     /locus_tag="PLC06_10180"
     CDS             5055..5741
                     /locus_tag="PLC06_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009366710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HRO90854.1"
                     /translation="MNIELLYRPAFSLGMIHLDGNERVRVEAASMVSMSQGVTLETQA
                     TGGFLKSLSRSVLGGQTFFQNTYTAPAGGGTVGVAPALTGDLMVINMQDELLMVQSGS
                     YLASAMGIELDTKWSGAKTFFAAEGLIMMRAQGSGLLLLASYGAIHEMNLAAGQSVTI
                     DTGHLVAFTEGIGFQVRKVGGMKSLLFSGEGLVVDLTGPGRLLMQTRSTDQFLSWLIP
                     KLPQSSGSSG"
     gene            5789..5989
                     /locus_tag="PLC06_10185"
     CDS             5789..5989
                     /locus_tag="PLC06_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90855.1"
                     /translation="MDRVVTHDWAEETLEAKAFWFQGLTLAERMELLCDYYEFFLSVN
                     PQIIESKDAQPIEGRILVLSEA"
     gene            5943..6386
                     /locus_tag="PLC06_10190"
     CDS             5943..6386
                     /locus_tag="PLC06_10190"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRO90856.1"
                     /translation="MLNQLKGVFSSFQKHEVRYLVIGGIAAVLYGVPRATFDLDVLIE
                     ATPDNSRRLLDALIDAQLGTAELTTPEQILDHEITIFRDRVRIDVQTMTPGIAFAEAW
                     SRKKTMTFQGQTFNVVSRDDLIASKLASGRPVDLEDVRLLRLSAE"
BASE COUNT         1274 a         2025 c         2024 g         1165 t
ORIGIN      
        1 ccctttcggg attatcctgt agttgtgcgc ggccgaattc acccgcgccg aggaaccctt
       61 cgatggacag ccaacaacat gaccaaaacg ccgaagaccg ttaccgtgag cgcagcgccg
      121 agcgcgccgc ccaacaggac aatgccgcca aagggatcgt cgatttcggc gagatgaccc
      181 gccgcgacgc ccgccgcgac acgctccacc ccaacgacgg gctggggctg gagcgcatac
      241 tgggcaacag cgacctgctg gagatcaact tcctcgatat cggccgtcgc gcggggcgag
      301 ccgtcggccg cgtcgagata cgcgacaccg ccgggcgcat ccgcggcttc ggcaccggct
      361 tcgtcgtctc gcccaacctg ctgctgacca acaaccacgt gctgcccacg gccgacagcg
      421 cccgccgcag cctgatcgac ttcgactacg aagacgacga gagctataac ccgcgctcgc
      481 cggtcgtgtt cggcctcgac cccgaccgct tctatcacac cagcgctgcg ctcgattaca
      541 ccgtggtggc cgtggggcgc gtgtcggtcg acagccaaac gccgctggcc tcgttcggct
      601 atctgcccct cagcgcctat caaggcaaaa tcctgctcga cgagtacgtc gccgtcattc
      661 agcaccccgg cggcgcgccg aagaagatcg ccctgcgcaa caacaagctc gtcgacttgt
      721 tcgacgactt catccactac actaccgaca ccgaccgggg cgcgtccggt tcgccggtgt
      781 tcaacgatca gtggcaggtg gtggggctgc atcacgccgg ggtgcgcaaa cgggacgccc
      841 agggacgcat cctggccgtc gacggctcgg tctggacgcc ggcgatgggc gaggatcgca
      901 tcgcctacat cgccaacgag ggggtgcgca tcagccgcat cctggccgat ctgcgcgccg
      961 cggccgatgg actatccgcc gaacaacagg tattgctgga cgaactgctg gccgagccgg
     1021 ccacgccgcc gaccaccgcc ggaaccgtca ccccgctgcg aacggccgag cgcgacccgg
     1081 agctgttcac gcgggccaag gggtatgaat ccggctttct ggggccggat gtggaactgc
     1141 ccgcgctctc cccggagcag ctggccgacg tggcgccgcg cctcgacggc cgcggccacg
     1201 tactggacta tgtgcatttt tcgctggtga tgagcaaggc ccggcggctg gccttctaca
     1261 cggcggtcaa catcgacggc cgcaagacca aaaccatcaa gcgcgacgac gacgtgtggt
     1321 acttcgatcc gcgcatcgcc cgcgaacacc agaccggggc cgacctctac gagcgcaacg
     1381 acctcgaccg gggccatctg gtgcggcgca atgaccccgt gtggggccgc ccggcggcca
     1441 cggccaacga ggacaccttc cacttcacca actgcgcccc gcagcacaag cagctcaacc
     1501 ggcgcacgtg gctggcgctg gaggattaca tcctcaacaa cgccgacaac catgacctga
     1561 aggtgtcggt tttcaccggg ccggtgtttc gggccgacga catgacctat cgcgggcagt
     1621 accagctgcc ggccgagttc tggaaggtag tggtgatgcg caagcccgac cggaagctgt
     1681 cggccacggc ctatctgcaa acgcaaaaga acctgatcga ggatttggag ttcgcctatg
     1741 gcgcgtatcg cacctatcag gtgccggtca cgcgcatcga ggcgatcacc gggctggatt
     1801 tcggccccct gcgcgaccac gacccgctgg ccgccacgga gagcgcctcg cctgcgctgg
     1861 tggtgggcga agcggccgac atccggttat aaactcgaac gtcgtgtcag gcgctcattc
     1921 cgccacgcga acaaggcgat gacgagcgcc acagccgcca gcccggccga caccagcgct
     1981 gtgccctgaa tgcccgcggc gatcgcttcc ggcgatcccg attccgcgcc gccggtcaga
     2041 atgccgcccg acgcgcggaa caccagcccc gcccagaccg accccaacac ggcgaagccg
     2101 accgtctgcc ccaggctgcg cgtcacggcc agcaggctgg aggcgacgcc gaggcgctcg
     2161 cggggcacgt tgcccatgat ggcgctgttg ttgggcgact ggaacatgcc cacgccgatg
     2221 cccagcggca ggaagcgcag catgaagccg atggccgtcg tttcggccgt cagcgtgctc
     2281 atcaacagaa aggcgaatgt ggaaacgaca aggccgatga ccgagattgg ccggctgccc
     2341 acgcggtcgg agagcgagcc ggagatgggc gcggtaatgc ccagcgccag cggcacgacc
     2401 gccaccagaa agccgacctg ctgcacgcta tatttcagcg cgttctcgag gtagaacggg
     2461 atgagcgcat aggtgcccat ggcgatgaag atcatcagcc cggtgataat attgacccga
     2521 aagacgcgat tgtcgaacag gctcaacggg atgatcggct gttcgtggcg gctctccagg
     2581 atgatgaacg tgaccagaaa gaaggcggcc acgatgaaca gccccagcgc ctgcgaactg
     2641 gtgaaccctt gcagctggcc ggtggtgagg ccgagcagga gcgccagcaa aaagacgcac
     2701 agcgtggccg ctcccgccag atcaaacacc tgatcgccat ggggtttcgt gtgggggatg
     2761 tggcgccatg ccagccaggt gccgataatg ccgatgggga cgttgacgaa gaaaatccag
     2821 tgccaggaca gatgctcgat gatcaccccg cccagcgtag ggccggtgac gatgccgatg
     2881 gagacgatgg agccggtgat gcccagcgcg cggccgcgct cgcccgcggg aaaagcgtcg
     2941 gtgatgatgg ccatacccaa cgccagggtc atcgccccgc caatgccctg cagcacgcgg
     3001 gccatgatga gccattccac cgtcggagat agcccgcaca gggccgagcc gatggtgaag
     3061 acgacgaagc cgccattata gaggtgtttg cggccgtgca tgtcggccag gcggccgaca
     3121 atcgcctgga ggatggtcac cgtcagcaag taggacagga cgacccattg caccgaggag
     3181 aagtcggtgt tgagttcgac cgtcagcgtc ggcagggcga cattgacgat gctgccgtcg
     3241 atcgtcgcca ggaagatacc catggcgatg gccgacatga cgtgccattt tttgctgtaa
     3301 tccacttcat cggccggcag cgcggccatc tctgctctgt ccatcannnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnntcgg cccggctgtg gtagtgcagc cgccacatct
     3481 cgcggcgcgg atgctcgacg tattcgaagc gggtcacgcc ggtgttcttg caccacacct
     3541 cgcagcgccg gaacgggccg acgttgaaaa tatggtcgag ggccagctcg atgaccccgc
     3601 catggcagac ggccaccacg acctggtcac gatggagttc gatcatgttc tcgatgaacg
     3661 aaccgacgcg aaccctgaag tgcatcaggc tctcgccgcc ctcgcggtcg ggcgtgattg
     3721 aggcaaacgg ccgttcgctg ccccaatagt cgctgtattc cggcaggtcg tcgctgggcc
     3781 aggggacgtg gtcggcgcgg ttgttgccga tctcgcgcag ccggtcatca tggatgatgt
     3841 ccatgttata ggcgcgggcc acatagccgg ccgtctcctg tgcccgaacc atggtgctgg
     3901 catagagcgc atcgatctct ttgagttcgg tcggcagcca ggtgcccagt gcagccgcct
     3961 ggcgatggcc caggtcggtc aggcctacat cctgattgcc gccgttccac tccttcagat
     4021 taacgaaact ttgtccgtgg cgaatgaaat acaaatgcat agcaactcaa ctcggttaat
     4081 tttcctgtta ctttagcgca tttgtgggca catgcccatc tctgcccgtg acttaaagtt
     4141 atttaaagat tctgcaatcg ttcgtggtca ggcgcttttg aactgttata aattaatgcg
     4201 gtatagctgc aggggcgggt ttcaaacccg cccctgcaac tcgatttgtt gatatgtaaa
     4261 agcggctggc tacgaaccgg agaggtcttt ttccgccacc tgccgcaaca cccggcccat
     4321 cacctccacc ggctgggcgc cgctgagggc gtatttgtcg ttgaagataa agaacggcac
     4381 gctgctgatg cccgcttgtt gggcgaaggc gatatcggcc agcacctggg cctcggccgc
     4441 gtcgccggcc aatgcggcgc ggatggcggc cgcgtcctgc ccctgctcgg cggcgatggc
     4501 ggccagcacg tcgggatcac caatgtcgcg gccgaactca aagtaggcgg cgaagatggc
     4561 gtcgagcatg gccccgcgct cggcttccgg cgtcagatag accagccggt gggagagcat
     4621 cgtgttgggc gctttggcaa tatcctcaaa gttgaacgtc aggccgaccg ccttgccgcg
     4681 ctggcggggc atggcgaaga aatcttcggg ggtcatgcgg ccgccgccct tggccgtcat
     4741 atgggcctgg aaatcgtagc cttccgcggg gatgtcgggg tcgaggaaga agctgcggtg
     4801 gcgaatagtg accggctcgc ccggccaatc ggccagagcc agttccagat ggcgcttgcc
     4861 gatgcggcac caggggcaga cggtgtcgga gaaaatgtcg attttcataa tacaaaataa
     4921 ttgaaaatta ggaattacga atcaggaatc gggaatcacg aatcctaatc tatactatct
     4981 tcatccgggc gcagcggcac gtttttgctc tttcacgcct tcatccgcgc ccattccgca
     5041 aaaggagaaa ccgcatgaat atcgaacttc tctatcgccc cgctttctcg ttgggcatga
     5101 tccacctcga cggcaacgaa cgggtgcgcg tcgaggccgc ctcgatggtc agcatgtcgc
     5161 agggggtcac cctggagaca caggccaccg gcggcttcct caagtcgctc agccgctcgg
     5221 tgctgggcgg gcagacgttc ttccagaaca cctacaccgc cccggccggc ggcggcaccg
     5281 ttggcgtggc cccggccctc accggcgacc tgatggtcat caatatgcag gacgaactgc
     5341 tcatggtgca gtccggctcc tatctggcgt cggcgatggg gattgaactc gataccaagt
     5401 ggagcggcgc gaaaacgttc ttcgccgccg aagggctgat catgatgcgg gcgcagggca
     5461 gcgggctgtt gctgctggcg tcctatgggg ccatccacga gatgaatctg gccgcgggcc
     5521 agtcggtcac catcgacacc ggccatctgg tcgccttcac cgaggggatc ggcttccagg
     5581 tgcgcaaggt gggcggcatg aaatcgctgc tcttcagcgg cgaggggctg gtggtcgacc
     5641 tgaccgggcc gggccgcctc ctgatgcaga cccgctccac cgaccagttc ctgagctggc
     5701 tcatcccgaa gctgccgcag agcagtgggt cgagtggttg agtggcgagt gacgagtgac
     5761 gggcaaggag ttatggtata attgcggtat ggatcgcgtg gtaactcatg attgggccga
     5821 agaaactctg gaagccaagg cgttttggtt tcaggggtta acgttggcgg agcgcatgga
     5881 actcttgtgt gactattacg agttcttttt gagcgtgaat ccccaaatta tcgagagcaa
     5941 agatgctcaa ccaattgaag ggcgtattct cgtcctttca gaagcatgag gttcgctacc
     6001 tcgtaatcgg gggcattgcg gccgtcctct atggtgtgcc gcgcgccacg tttgatcttg
     6061 atgtcctgat cgaagccacg cccgataatt cgcgccggtt gctcgatgcc ttgattgatg
     6121 ctcaactcgg cacagcggaa ttaaccaccc ccgaacagat tctcgaccac gagatcacca
     6181 tctttcgtga cagggttcgc atcgatgtgc agacgatgac gcccggcata gcgtttgctg
     6241 aagcgtggtc tcgcaagaag acaatgacgt ttcaaggtca gacattcaat gtggtctcac
     6301 gcgatgacct gatcgcttcc aagctggctt caggacgtcc cgttgatctt gaagatgtgc
     6361 gcttgcttcg tctaagcgcg gagtgagtgg accgacgacc gacgacaaag agaacgctct
     6421 tgtcggggtc ggtggccggc ggacgggagc ctgcatggcc ctgttgtacc ccgtccctcc
     6481 gccaaacaaa aaagccgcgc tggtcacaat cgcaaccggc gcggctttct tcaattcgta
     6541 attcgtaatt cgtaattcgt aattcgtaat tcgtaattcg taattcgt
//
LOCUS       DAOMQG010000175         6574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5011_length_6574_cov_10.876822, whole genome shotgun sequence.
ACCESSION   DAOMQG010000175 DAOMQG010000000
VERSION     DAOMQG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_6574_cov_10.876822"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1807
                     /locus_tag="PLC06_10195"
     CDS             92..1807
                     /locus_tag="PLC06_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HRO90857.1"
                     /translation="MSSSSMNLSAQGGAWEGKYWNNRNLSGAPVLTRQDASINFDWGA
                     GSPASGIGADEFSAQWTQTANLPAGTYRFTATTDDGMRVWIDNVMIIDAWFDSQQHTI
                     TADVFLGAGNHTIVVQYYEAGGLAVAKMNYVLLGGPTPPPVSDQTWYNEYFANTNLSG
                     TPTLTGTTSAVNFNWGFGSPAPGFPADFFSVRWVRNVPLEAGRYRFTVTSDDGVRVWV
                     NNNLIIDQWRTQAAATFNAEIDLPGGNVPIKVEYYENTERAQIALSWTRLSAPPPQPT
                     GAYFAEYFNNMTLSGTPVLTRNEAAVNYNWGHGSPAPQVPVDNFSARWTATLNLSPGR
                     YRFSASSDDGVRLWVNDHLLIDAWFDQPVRTFTGEYDVTGPTRVRIEYYENTNLAEMR
                     FSYALVSSQPGTGGPFPGTATVTAYRLNVRRGPGANFAIITRLSNGNVVNLTGFRNGD
                     ATWVQVALPNGTIGWVSALYVRTTVPVGNLTPITGTTPPTPPPSGGATGTVNTGALNV
                     RTGPGVNFPAFTTIDNGQNVTLLGRNSAATWVRIIMNDGRQGWVNVSFLIINVPVGSL
                     PVLNN"
     gene            1948..2502
                     /locus_tag="PLC06_10200"
     CDS             1948..2502
                     /locus_tag="PLC06_10200"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HRO90858.1"
                     /translation="MFTFDLSPLTSDVLMGATLIFLLRVFGVAISTIRVLVMMRGKKV
                     AAFIAGFFEVLAYVIAIGEVVNNLDNIWNVLGYCLGFSVGTVAGMLLDERTASGFANV
                     RIISRYKAQSIIDEIHKSGFGATVGWGHGRGGTVGMIVAVVRRKEVDKICAIAESIDP
                     NAFITIEDARAVRRGFIQLSYQER"
     gene            2530..2772
                     /locus_tag="PLC06_10205"
     CDS             2530..2772
                     /locus_tag="PLC06_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90859.1"
                     /translation="MSAITLLDGSLKLSIYYDTMDKEFDDDICLCFQEDCPEDEKVLG
                     ADEVSIYITPEQAALVALALNRAIEAHRLDAKTSDR"
     gene            2843..3694
                     /gene="panB"
                     /locus_tag="PLC06_10210"
     CDS             2843..3694
                     /gene="panB"
                     /locus_tag="PLC06_10210"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRO90860.1"
                     /translation="MGRKKITIIDLQSKKEHQEPISMLTAYDYTGALLVDRAEIDMIL
                     VGDSLGMVMMGLDSTVPVTMDEILHHCRAVARGAKFAHLVGDLPFMSYQADKYDAVRN
                     AGRLLKEGNMDSVKLEGGREMASTVRAIVDAGIPVMGHIGLTPQSISKLGGFKAQGRT
                     ATAAARLVEDALALEEAGCYGVVLEAVPAPVAAAITERLHIPTIGIGAGPDCDGQVLV
                     FHDILGLYDKIQPRFVKQYARLQEPIIEAFTQFRDEVTNRHFPSKEHSFSMSAEELEA
                     FHQSLAE"
     gene            3703..4692
                     /locus_tag="PLC06_10215"
     CDS             3703..4692
                     /locus_tag="PLC06_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRO90861.1"
                     /translation="MKVGVIGVGALGCLLGAYLGENGDVIMIGHWLDQVTTIKTSGLR
                     VRVPDGRVATHFPAITANPEEAGPVDIALVVVKSRQTREAAHLAARILKPDGLAITLQ
                     NGLNNCDTLREVLGEHRATLGVTSQGATVLGVAEIMHAGHGPTYFGRDLSLGDNQLAL
                     LPQIVELFNQSGLEAHLVDETEGLVWGKLAVNAAINPLTALLRVPNGFLVKHEVLIDI
                     MKRAAREVENVANAQGITLPYPDASGRALEVARATAPNRSSMLQDVDRGAPTEVEAIC
                     GAVARIARDLGIQTPVNLRLCRLVRQIEGGQPPLLEQGDVAGLLQLLDLPTLE"
     gene            4698..5567
                     /gene="panC"
                     /locus_tag="PLC06_10220"
     CDS             4698..5567
                     /gene="panC"
                     /locus_tag="PLC06_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRO90862.1"
                     /translation="MIVVETIEEVRAVRQRYALETLGLVTTMGYLHQGHLELVRQAKA
                     ENDRVAATIFVNPKQFGPTEDLSRYPRDLQRDLELLEAEGVEYVFVPTDETMYPADFQ
                     TVVSVTGITKYLEGARRPTHFDGVTTVVAKLFNIMQPTRAYFGQKDAQQVVVVRQMVR
                     DLNFNLEIVVCPTVREADGLAMSSRNKYLSAEQRQAATALYRALGSAEALWHDGERHS
                     DALRDSMAQVLANESLARVDYVSAADPDTLREAEGEFPPEKGALLSMAVFFDKTRLID
                     NFLLAPTEPNQHT"
     gene            5929..6294
                     /locus_tag="PLC06_10225"
     CDS             5929..6294
                     /locus_tag="PLC06_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO90863.1"
                     /translation="MKTILQIEDNFANRRLVERVLEPYSYRLINAIDGQSGIDTALME
                     APDLILLDMGLPDMDGQTLVSVLRETPSLAKVPIIALTAWPESIAGEMALRYGCDGCI
                     TKPINVAEFGKQIEVYLSR"
     gene            6324..>6574
                     /locus_tag="PLC06_10230"
     CDS             6324..>6574
                     /locus_tag="PLC06_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90864.1"
                     /translation="MILLSASDFAIEELTRAYNQTRTDYLIPMPMNPGRLQEYILLYD
                     VALDRSKVAIEGDNIVGLGMLGKRKGMGWITRLGVLPDG"
BASE COUNT         1491 a         1773 c         1904 g         1406 t
ORIGIN      
        1 aggagcgtca tcaatggata ggtggcatgc atttggaaga gcacgttttg tccttttagc
       61 actgtttctc tccgtgctgg caatcgcaac attgtccagc agttcaatga acttgagcgc
      121 ccagggtggc gcatgggaag gaaagtactg gaacaaccgg aatctctccg gtgctccggt
      181 attaactcgc caggacgcga gcatcaactt cgattggggc gccgggtcac ccgcgagcgg
      241 aatcggggcg gatgagttct cggctcaatg gacccaaacc gccaatctgc cggccggcac
      301 ctatcgcttc acggcgacga cggatgatgg catgcgcgtg tggatcgaca acgtgatgat
      361 catcgacgcc tggttcgata gtcaacagca cacgatcacg gccgacgtct ttctgggagc
      421 cggcaaccat acaattgtcg tccagtatta tgaagccggc ggcctggccg tcgcgaaaat
      481 gaattacgtc ctcttggggg ggccgacccc gccgccggtc agcgaccaga cctggtacaa
      541 cgaatacttc gccaacacca acctcagcgg gacaccaacg cttaccggca caacatcggc
      601 cgttaatttc aattggggct ttggctcccc ggctccgggc ttcccggcag atttcttctc
      661 ggtgcgctgg gtgcgaaatg tccccctgga agcgggtcgg tatcggttca ccgttacctc
      721 tgacgacggc gttcgcgtgt gggtgaacaa caatctgatc atcgatcaat ggcgcacaca
      781 ggccgcggcc acattcaacg cggaaatcga tctgcccggc gggaatgtcc cgatcaaggt
      841 agagtattac gagaatacgg aaagagccca gattgctctg tcctggacga ggctatccgc
      901 cccgcccccc cagccaacgg gcgcgtactt cgccgaatac ttcaacaaca tgaccctttc
      961 cggcacgccg gtgttaactc gcaacgaggc ggccgtgaat tataactggg gtcatggttc
     1021 cccggcgccg caagttcctg tcgacaattt ctcggcgcgc tggacagcaa ctctgaacct
     1081 gtcgcccggc cgctatcgtt tctcggcctc cagcgatgac ggtgtgcgtc tgtgggtgaa
     1141 cgatcacctg ctcatcgatg cctggttcga tcaacccgtt cgtaccttca ccggtgaata
     1201 cgacgtcacc ggcccgaccc gggtgcgaat cgagtattat gagaacacca atctggccga
     1261 gatgcggttc tcctacgcgc ttgtctccag ccaaccggga acaggtgggc cgttccccgg
     1321 cacagccaca gtgacagcct atcggctaaa cgtgcgtcgc gggccagggg cgaatttcgc
     1381 catcatcacc aggctaagca acggcaacgt ggtgaacctg accggcttcc gcaatggtga
     1441 tgccacctgg gtacaggttg ccttgcctaa cggaacgatc ggctgggtaa gcgcgttgta
     1501 cgtgcgaacg acagtaccgg ttggcaacct gacgcctatc acaggcacga caccccccac
     1561 cccaccacca tccggcggag caacaggaac ggtgaataca ggcgcgttga acgtcaggac
     1621 aggcccgggc gtcaacttcc ctgccttcac aacgatcgac aatggtcaga acgttacctt
     1681 gttgggccga aacagcgcgg caacctgggt aaggatcatc atgaatgacg gccgacaggg
     1741 ctgggtgaac gtatcgttcc tgattatcaa tgtccccgtg gggagcctcc cggttctcaa
     1801 taattaacga ttagggaccc ttcgcaagct tcaaagcctc cccaagcgtt ttggggaggc
     1861 ttttgtgtat tggccaggaa acggcgcgag gactaaaata ggcttgtcgt aagctgaacc
     1921 acactttccg gagagcagac tctttccatg ttcacttttg acctttcccc actgaccagc
     1981 gatgtcctca tgggggcgac cctgatattt ctcctgcgcg tgtttggcgt ggccatcagc
     2041 accatcaggg tgctggtgat gatgcgcggg aagaaagttg cagcattcat cgccggcttt
     2101 ttcgaggttc tggcctatgt gatcgccatc ggcgaagtgg tgaataatct ggataacatc
     2161 tggaacgtgc tgggatattg tcttggcttt tcggttggca cggtggccgg catgcttctg
     2221 gatgaacgca cagcgtccgg cttcgccaat gtccgaatca tctctcgcta taaagcgcag
     2281 agcattatcg atgagatcca caagtcggga tttggggcga cagtcggctg gggccacggt
     2341 cgcggcggga cggttggcat gatcgttgcc gtggtgagaa ggaaagaagt agacaagatt
     2401 tgcgccattg ctgagtcgat tgaccccaac gcctttatta caattgagga cgcacgcgct
     2461 gttcgccgcg ggtttattca attgagttac caggaacgat agccgataga accgataaag
     2521 ggggtgatca tgtccgctat aactctgctg gacggtagcc taaagctttc gatttattac
     2581 gacacgatgg acaaagagtt cgatgacgac atctgcctat gcttccagga agattgcccg
     2641 gaagacgaga aggtgctcgg agcagacgaa gtgagtatct acatcacgcc ggagcaagca
     2701 gcgctcgtcg cgctggcgct caatcgggcg atagaagctc atcggctcga tgcaaaaacg
     2761 tctgatcgct gaagagaatc acaacaaatt agaactccta ttccagccac atcactcgaa
     2821 taaggagttt gaggattatg atatgggccg caagaaaata acgatcattg atcttcagtc
     2881 aaaaaaagaa caccaggaac ccatcagcat gttgaccgcc tatgactaca cgggcgccct
     2941 gctggtggac agggctgaga tcgacatgat cctggttgga gactccctgg gaatggtcat
     3001 gatgggcctg gatagcaccg tgcctgtgac catggatgaa atactccatc actgccgtgc
     3061 cgtggcgcgt ggggcaaaat tcgcccattt ggtgggcgat ctgcctttta tgtcctatca
     3121 agccgataaa tatgacgccg tccgcaatgc cggccgcctg ttaaaagaag ggaacatgga
     3181 tagcgtgaag ctggagggcg gccgcgaaat ggcgtcaacg gtgcgcgcta tcgtcgatgc
     3241 aggaattccg gtgatggggc atatcggctt gacgccgcaa tcgattagca aattaggcgg
     3301 gttcaaggcg cagggccgaa cggcaacagc cgcggctcgt ctggtagaag atgcgttggc
     3361 cttggaagaa gccggctgtt atggggttgt tctcgaagct gtgcccgcac cggtagcggc
     3421 cgcaattacc gaacggctgc acatcccgac catcggcatc ggcgccgggc cggattgcga
     3481 tggccaggtg ctcgtgttcc acgatattct tggcctctat gacaagattc agccgcgttt
     3541 tgtaaagcag tatgccaggc tccaggagcc aatcattgag gccttcaccc aattccggga
     3601 cgaggtgact aaccggcatt tcccgagcaa agaacattca ttctcaatga gcgcagaaga
     3661 gttggaagca tttcatcaaa gcctggccga atgacaactt ctatgaaggt cggcgtaata
     3721 ggtgtaggcg ccttgggctg cctgttaggc gcttatctgg gcgagaatgg cgatgttatc
     3781 atgatcggcc attggctgga tcaggtcaca acgatcaaaa catcggggtt gcgggttcgg
     3841 gttcctgatg gccgggtagc gacccatttc ccggccatta ccgccaatcc cgaagaagct
     3901 gggccagtcg atatcgccct cgtggtggtg aagagccggc agacgcggga agcggcacat
     3961 cttgccgcgc gaatcctcaa gcccgatggc ctggcaatca ccttgcagaa tggcttaaac
     4021 aactgcgata cgctgcgcga ggtgctgggc gagcatcggg ccacgctggg cgtcacctca
     4081 caaggtgcca cggttctggg agtggctgaa attatgcacg cgggccacgg tcctacctat
     4141 ttcggccgtg atctatcgtt aggcgacaat caattagcac tcttgccaca aatcgttgag
     4201 ctatttaatc aatcaggcct ggaggctcac ctggtcgatg agaccgaagg actcgtatgg
     4261 ggcaagttgg cggtgaacgc agccatcaat cccctcacgg ctctgttgcg cgttcccaat
     4321 ggctttctgg ttaagcatga ggtgctgatc gatatcatga aacgagcggc tcgggaagtc
     4381 gaaaacgtgg ccaacgcgca agggattact ctgccctacc ccgacgcttc cggacgcgcg
     4441 ttggaggtgg cccgagccac agccccgaat cggtcctcca tgctgcagga tgtggatcgc
     4501 ggcgcgccaa ccgaggttga agcgatctgt ggcgctgtgg cgcggatcgc gcgggatctg
     4561 ggcattcaga cgccggtgaa cctgcggcta tgccggctcg tcaggcaaat tgaaggcggc
     4621 cagcccccgc ttctggagca aggagatgta gccgggctgc tgcagctgct cgatttacca
     4681 acgttggagt aatcacgatg atagttgtcg aaacaattga ggaagttcga gccgtacgtc
     4741 aacggtatgc cctcgaaacc ttgggccttg tcacgacgat ggggtatctg catcagggac
     4801 atcttgagct tgtacgccag gccaaagcgg agaatgatcg cgttgccgcc acgattttcg
     4861 tcaaccccaa gcaatttggc cccaccgaag atttgagccg ataccccaga gatctgcaac
     4921 gcgatttgga gctgctggaa gcggagggag tggaatacgt ttttgtaccc accgatgaaa
     4981 cgatgtaccc tgcggatttt caaactgtag tgagcgtgac cgggatcacg aaatatttgg
     5041 aaggcgcccg ccggccgacg catttcgatg gcgtgacaac ggttgtggcc aagctgttta
     5101 acatcatgca gccgacccgc gcatattttg ggcaaaaaga cgctcagcag gtcgtcgttg
     5161 tccggcaaat ggtgcgagac ctgaacttca atctggagat cgtcgtttgc cctactgtgc
     5221 gcgaggcgga tggactggcg atgagttcac gaaacaagta tctgtcggcc gagcaacgtc
     5281 aagcagcaac ggccttatat cgcgctctag gcagcgctga ggcgctgtgg cacgacggtg
     5341 agcggcatag tgatgcatta agggattcga tggcgcaggt gttagcgaac gagtcattgg
     5401 ctcgcgttga ttacgtaagc gcggccgatc ctgatacgct gcgcgaggca gagggagagt
     5461 tcccccctga aaaaggcgct ctcctgtcaa tggcagtatt ttttgacaag acccgcctga
     5521 tcgataattt cctgctcgcc ccaacagaac cgaatcagca cacataaaag tatcctcaat
     5581 cgccacactt cagttggccc gaattcaaac ctctgttatt atcgaactac cttttccgtc
     5641 aggtaaccgt atgactagtg ctcaggattc ctctccaatc agcccatatt tgcttgagat
     5701 tttgcgttgc cctgttgccg ttcgggccaa agaacacgcc ggcgacgacc ctggtcgttt
     5761 gcgtctcgta ggagcgtatt ggctcgtatg cgacgaatcc gggatgaaat atccgatcaa
     5821 gaacggcata ccgatcatgc tcattgaaga aggggaaaag tggcgggata aagcggagag
     5881 cgatttgccg attccgcctc cggtgagtta atgtgccatg accgactcat gaaaaccata
     5941 ttgcaaatcg aagataactt tgcgaatcgt cgattggttg agcgcgttct cgaaccttat
     6001 tcgtatcgct tgataaacgc cattgatgga cagtcgggta ttgatacggc gctgatggaa
     6061 gcccctgatc tgatattgct cgatatggga ctgcctgata tggacggcca aacgctggtc
     6121 tcggtgctgc gagaaacgcc gtcgctggcg aaagtcccca ttatcgcgct cactgcgtgg
     6181 ccggaaagta tcgcggggga aatggccctt cgatatggtt gtgacggatg catcacaaag
     6241 cccatcaacg tcgcagagtt tggcaagcag atcgaggtct atttgagccg ctagatagtg
     6301 gccgagcaag cagcaaacac cttatgatcc ttctatcggc cagcgatttc gccatcgagg
     6361 aacttacccg agcctataat caaactcgaa ccgattacct tatcccgatg ccgatgaatc
     6421 ctgggcgcct gcaggaatac atcctgctct acgacgtggc gcttgacaga tcgaaagtgg
     6481 caattgaggg ggacaacatt gtcggtctgg ggatgctcgg caaacggaaa ggcatggggt
     6541 ggattacgcg gcttggtgtc ctccccgacg gcag
//
LOCUS       DAOMQG010000176         6481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5097_length_6481_cov_10.670090, whole genome shotgun sequence.
ACCESSION   DAOMQG010000176 DAOMQG010000000
VERSION     DAOMQG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5097_length_6481_cov_10.670090"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1299
                     /locus_tag="PLC06_10235"
     CDS             16..1299
                     /locus_tag="PLC06_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO90865.1"
                     /translation="MRPFFTIWIGQMFSLLGSQLVQFALIWWLTQQTGSATVLAGASI
                     VGLVPQVILGPFVGPLIDRWNRKWTMILADGAVALSTGVLVYLFWTGAVEVWHIYAIL
                     FIRALGGSFHWPAMTASISLMVPQEHLTRVQGLNQILNGGLSIASAPLGALLISLLPM
                     ENVLMIDIVTAAIAMVTIAFVHVPQPEARQAQVGETSASYLADLRAGLRYILNWRGLM
                     LLGGMSMLINMVLGPTTSFMPLLITDHFSGTAWHLGLIEAAFGIGVLAGGILLSVWGG
                     FKRRIYTSLVGLGGIGLGILVVGLAPANLFSLAVAGMAFGGVMSSLCNGPVMAIFQST
                     VAPSMQGRVFTLIGSASAAMMPLSLALAGPIADIIGVRTWFLVGGLATLALCLIAPFI
                     PSLVNIERDRDDFVEETGRVAVPHLAPVVKAAEKA"
     gene            complement(1382..1762)
                     /locus_tag="PLC06_10240"
     CDS             complement(1382..1762)
                     /locus_tag="PLC06_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90866.1"
                     /translation="MAIIQSIHGALSNTAWLFFLIIGLWGLFRAIRGQAVNGNYLGAA
                     FIGQLIFVAQAVLGAVLWIGGFSATIQRSSIHLLYGVFALVFLPFIYLSVLRGDDTNR
                     AQWVLAFTTLFLFGIALRAITTAA"
     gene            1864..3246
                     /locus_tag="PLC06_10245"
     CDS             1864..3246
                     /locus_tag="PLC06_10245"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRO90867.1"
                     /translation="MDSYQRALDYLYSFINFEHKVLDRYQASKIDADRPRRLLSLLGD
                     PHRQYPSIHIAGTKGKGSVAAMCATALCAAGLRVGLYTSPHLREFRERIRVIGPEDAD
                     GRIPEEAFVTQMDRVREHLDEVPGITWFEIVTAIAFMYFADAGVDVVVAEVGLGGRLD
                     ATNVLLPEVSVITSLSLDHTQLLGDTLAQIAYEKGGIIKPGVPVVTAMQKPEALKTLE
                     QIAAERDSPLTVVGRDWQYQVSRESKSGHVEQRLIVENNRAPDLIPRGSEFTVALSGA
                     HQLENAAVALATLETVHGRFPGLDIHALHVGLSTVRWDGRLQVLQQGADCPTVLVDCA
                     HNDDSAARLVSAVTADFDYRRLILIFGAPEDKNVAGMLSRLLPIADRIIMTTSNHPRS
                     ATPEQLVAMAADMGREASMTHSVAEALDQALGEARPGDLVVAAGSIIVIGDLLNHWDT
                     LQSDTTTNHG"
     gene            3239..>3573
                     /locus_tag="PLC06_10250"
     CDS             3239..>3573
                     /locus_tag="PLC06_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRO90868.1"
                     /translation="MDDFDFSQFPDFPELFPDLGEDIFDQPVTKDKDGIIECPFLALR
                     DIVLFPQMVMPLFIGRERSLAAINAANTKRENLIVATQRDSNVVDPDESGLFEIGTET
                     TIGRLLRMP"
     assembly_gap    3574..3673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3674..5484
                     /gene="lon"
                     /locus_tag="PLC06_10255"
     CDS             <3674..5484
                     /gene="lon"
                     /locus_tag="PLC06_10255"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRO90869.1"
                     /translation="QQILEILEPHTRLQTISVLLAKELDVLELEDEIQSQVQQEVDRS
                     HREHFLREQMRIIQGELGELDVFGQELSELRDAVDKKNLPEGVRAKMEKELARLNAMP
                     PMSPEIGIIRTYIDWILDLPWLEESADNLEVGNAAAVLDADHYGLERVKDRILEYIAV
                     RRIAPDSLRSPILCFVGPPGTGKTSLGRSIARAMGREFVRISLGGVRDEAEIRGHRRT
                     YIGAMPGRIIQAMRRVGTRNPLFMLDEVDKLGADFRGDPSAALLEVLDPEQNNSFVDH
                     YLGLEYDLSRVLFVTTANYLDAIPHALQDRMEVIEFSSYLEEEKLGILHQFLIPRQLE
                     QHGLATKNVTFTEDGLKTLIREYTREAGVRNLEREVANVCRKIARQVAERKRYPRQIT
                     PDKVRELLGPPRFTEDIRPDVDEVGVATGAAWTAAGGELLIIEVNLMPGKGNLILTGQ
                     LGDVMRESAQAALTYTRSQVDALGIEAERFERQDIHIHLPEGAVPKDGPSAGATLATA
                     LISAFTGRSIRHDVAMTGEITLRGRVLPVGGVREKALAARRAGIRTFILPRKNERDLL
                     DIPKHLRQDIKFVLVDRIEEVLAAALLPPTSRKQEF"
     gene            5484..6365
                     /locus_tag="PLC06_10260"
     CDS             5484..6365
                     /locus_tag="PLC06_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRO90870.1"
                     /translation="MAHNMPIATFSIVAYDPRKKEWGVAVQSKFLACAAVVSWARAGA
                     GAVATQSFANIAYGIDGLDMMERNISAADTIEALTTADENRAQRQVGMVDKNGRAGAF
                     TGDGCYDWAGHIVGEGFTCQGNILVPGTVEAMAATFEAARRGPGELADWLVEALAAGQ
                     AAGGDSRGRQAAGVMVVRHNGGYGGNNDRYLDLRVDDHPEPIEKLAELVTMHHLLFGE
                     VDPDKLVSLAGVAGDLQKVLRRSGHLTRDVDGQFDDETREALRALVGSENLEERWDGT
                     GEMIDSVVVDFLMRRYG"
BASE COUNT         1233 a         1779 c         2007 g         1362 t
ORIGIN      
        1 tcaaccccga aatccatgcg gccctttttc acgatttgga tcgggcagat gttttccctg
       61 ctcggaagtc agcttgttca attcgcgctg atctggtggc tgactcagca aaccggttcg
      121 gcgacggtgc tcgctggtgc ctctattgtc ggactggtgc cgcaggtgat tcttggccct
      181 ttcgttggcc ccctgatcga tcgctggaat cggaagtgga cgatgattct ggctgatgga
      241 gcagtcgcgc tttcgacggg cgtccttgtt tacctcttct ggacgggggc cgttgaggtc
      301 tggcacattt acgcgattct gttcattcga gcactgggcg gctcattcca ttggccggcc
      361 atgaccgcat cgatctcgct catggtgccc caggagcatc taacgcgagt tcagggtctg
      421 aatcagatcc tgaacggcgg gttgagcatc gcctcggccc ccctcggcgc gctgttgatt
      481 tccctgttgc cgatggaaaa tgtgttgatg atcgatatcg tcaccgcagc cattgcgatg
      541 gtaacgatcg cgtttgtcca tgtgcctcag cccgaggcca gacaagctca ggtgggtgag
      601 acctcggcgt catatctcgc cgatttgcgg gccggccttc gctacatcct gaactggcgt
      661 ggcctcatgt tgttgggagg catgagcatg ctgatcaaca tggtccttgg cccgacgacc
      721 agctttatgc ctctgctgat aaccgaccat ttttccggta cggcatggca tctgggcctc
      781 attgaagcgg ctttcggtat cggcgtgtta gctggtggga tcttgttgag cgtgtggggt
      841 gggttcaaac gacggattta tacgtcactg gtcggtcttg gtggaattgg gctgggcatt
      901 ctggtcgttg gcctggcccc ggcaaacctg ttttcgctgg ctgttgccgg gatggcgttt
      961 ggcggagtca tgagttcgtt gtgtaacggg ccggtcatgg cgatatttca atcaaccgtt
     1021 gcgccttcga tgcaaggacg ggtgttcacc ctgattggca gcgcttcggc ggccatgatg
     1081 cccttgagtt tggctctcgc cgggccgata gccgacatca tcggcgtgcg cacctggttt
     1141 ctggtcggcg gcctggccac cctcgcgctc tgcctgattg ctccctttat tccgtcgttg
     1201 gttaatatcg agcgggatcg ggatgatttt gtggaggaaa cagggcgcgt ggctgtcccg
     1261 catctcgcac ccgtagtcaa ggcggccgaa aaagcctgat tcgtcacagt tggtggactg
     1321 ctccgtcttt ttgcggaaat atgtaccccc gagtgaaatc cgggggtttt gttattgatg
     1381 atcaggcggc cgtcgtgatg gcgcggaggg cgatgccgaa caagaacagg gtcgtgaacg
     1441 ccagcaccca ctgtgcccgg ttggtgtcat cgccgcgcag gacggacaga tagataaacg
     1501 gcagaaacac cagcgcaaat acgccgtata acagatggat gcttgaacgc tggattgtgg
     1561 cgctgaagcc gcctatccag agcacggctc ccaaaacggc ttgagctaca aaaatcagtt
     1621 ggccgatgaa agccgccccc aggtaattgc cgttgactgc ttgtccacgg atggctcgaa
     1681 acaagcccca aaggccaatg atcaggaaaa agagccaggc ggtattggat aacgccccat
     1741 gaattgattg gatgattgcc attatttgat gttcctttcg gccgcgagtg tagctttccg
     1801 ccagatgctt gtcaaagcgt attcactgtc atggccccct cggatgggtt acaatcaggg
     1861 gatatggatt catatcaacg tgcgcttgat tatctctata gcttcatcaa cttcgagcat
     1921 aaagtgctcg atcgctatca ggccagcaag atcgatgccg atcggccgcg ccgtttgtta
     1981 tcgttgctcg gcgatcccca ccggcagtat ccgtcgatcc acattgccgg caccaagggc
     2041 aagggatcgg tcgcggcgat gtgcgccacc gctctgtgtg cggccggctt gcgcgtcggg
     2101 ttatatacat cgccccatct gcgcgaattc cgcgagcgca ttcgcgtgat cggccctgag
     2161 gacgcagacg gccgcatccc ggaagaagcg ttcgtcacgc agatggatcg tgtccgggaa
     2221 caccttgatg aggttcccgg catcacctgg ttcgagatcg ttacggccat cgctttcatg
     2281 tactttgccg atgctggagt tgacgtagtc gtggctgagg tcgggctggg cggccgcctg
     2341 gacgcgacga acgtcctgct gccggaggtc tcggtcatca ccagcctgag cctcgaccac
     2401 acccaactgc tgggggacac gctggcccag attgcctacg aaaagggggg gatcatcaag
     2461 cctggcgttc ccgttgtcac agccatgcag aaacccgaag cgctcaagac tctggagcag
     2521 atcgcggcgg aaagggattc tccgctgaca gttgttggcc gcgactggca atatcaagtc
     2581 tcgcgcgaga gcaaatccgg tcacgtggaa caacggttga tcgttgagaa taatcgtgcg
     2641 cctgacctca tccctcgggg aagcgaattc actgtagctc tttccggcgc gcatcaattg
     2701 gagaacgcgg ccgttgcgct ggcgacgctg gagacggttc atgggcgctt cccaggcctt
     2761 gacattcatg cgttgcatgt tgggctatcg accgttcgct gggatggccg cctgcaggta
     2821 ctgcagcaag gcgcggattg cccgactgtg ctggtcgatt gcgcgcataa tgatgattcg
     2881 gctgctcgac ttgtgagcgc ggtgacggcc gattttgact accggcgatt gattctgatc
     2941 tttggcgcgc cggaggacaa gaacgtcgcc gggatgctgt ctcgcctgct gccgatagct
     3001 gatcgaatca tcatgaccac gtccaatcac ccccgctcag ccacgccgga gcaacttgta
     3061 gccatggccg ccgatatggg acgggaggca tcgatgacac actcggtggc tgaggcgctg
     3121 gatcaggcgc tgggagaagc ccgacccggc gatctcgttg tcgccgccgg ctcgatcatc
     3181 gtaatcggcg atttgcttaa tcattgggac accctacaat ccgacacgac tactaaccat
     3241 ggatgatttc gacttcagcc agtttcccga ctttcccgaa ctcttccccg atctgggcga
     3301 agacatattt gatcaacctg tcacgaaaga caaggatggg ataattgaat gcccgttcct
     3361 ggctttgcgc gacattgtcc tgtttccgca gatggtgatg cccttgttca tcgggcgcga
     3421 gcgctcgctg gcggctatca acgcggcgaa taccaagcgc gagaatctca ttgtcgccac
     3481 ccagcgggat agcaatgtcg tggatcccga cgagagcggg ctttttgaga tcgggacaga
     3541 gacgacgatc ggccgcctgt tgcgcatgcc ggannnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnggcagca gatcctggaa atccttgagc cgcatacccg actgcaaacg
     3721 atcagcgtgc tgctggccaa ggagcttgat gttctggaac tcgaagatga gatccagagc
     3781 caggttcaac aagaggtcga tcgctcccat cgggagcatt ttctccgcga gcagatgcgc
     3841 attatccagg gcgagctggg cgagctggac gtctttgggc aagagctgag tgaactgcgc
     3901 gacgcggtgg acaagaagaa cctgcccgag ggcgtcagag ccaagatgga gaaggagttg
     3961 gcccgcctca atgccatgcc gcccatgtcg ccggagatcg gcattattcg cacctatatc
     4021 gactggattc tggatttgcc gtggctggaa gagtcggccg ataatctgga agtcggcaac
     4081 gcggccgcgg tgctcgacgc cgatcattac ggcctggagc gggtgaaaga tcgcatcctg
     4141 gagtacattg ctgtccgtcg catcgccccc gacagcctgc gcagcccgat cctgtgcttt
     4201 gtggggccgc ccggcacggg caagacctcg ctcggccgct ccattgcccg tgcgatgggt
     4261 cgggaatttg tgcgtatcag cctcggcggc gtgcgcgatg aggcagagat ccgcggtcac
     4321 cggcgcacct atatcggcgc gatgccggga cggatcatcc aggccatgcg tcgcgtcggc
     4381 acgcgcaacc cgctttttat gctggatgag gttgacaagc tgggcgccga ctttcgtggt
     4441 gatccgtcgg ccgccttgct ggaagtgctg gaccccgagc agaataactc cttcgtcgat
     4501 cattatctgg ggctggagta tgatctgtcg cgcgttctgt tcgtgacgac ggccaactac
     4561 ctggacgcca ttccccacgc cctgcaggat cgtatggaag tcattgagtt ctccagctat
     4621 ctggaagagg agaaactcgg tatcctgcat caattcctca tcccgcgtca gttggagcag
     4681 cacgggctgg cgaccaaaaa cgtgaccttc accgaagatg ggctgaaaac gctgattcgc
     4741 gagtacactc gggaagccgg cgtgcgcaac cttgagcgag aagtggctaa cgtctgccgc
     4801 aagatcgctc gccaggttgc ggaaagaaag cgctaccccc ggcagatcac acccgacaag
     4861 gtgcgtgagc tgctgggtcc gccgcgcttt accgaggata tccggcctga tgtggacgag
     4921 gtgggcgtgg ccacgggggc ggcctggaca gcagcgggcg gcgagttgtt gattatcgag
     4981 gtgaatctca tgcccggcaa agggaatctg atcctcaccg ggcagttggg cgacgtgatg
     5041 cgcgagagcg ctcaggcggc gctgacctac acccgtagcc aggtcgatgc cctggggatt
     5101 gaggctgaac gcttcgagcg acaggatatc cacattcatt tgcccgaagg agccgtgccg
     5161 aaggatgggc catcggccgg ggccacgctg gcgacggcgc tcatttcggc cttcaccggg
     5221 cgatcgatac gccacgatgt tgccatgacg ggtgaaatca cactgcgggg gcgggtcctg
     5281 cccgtcggcg gcgtgcgtga gaaagcattg gctgctcgcc gggccggcat ccgcaccttt
     5341 atcctgccac gcaagaacga gcgagatctg ctggatattc ccaaacatct gcgacaagac
     5401 attaaatttg tgctcgtgga tcggatcgaa gaagtgctgg cggcggcgct cttgccgccc
     5461 accagtcgga aacaggagtt ctaatggccc ataatatgcc catcgccaca ttctcgattg
     5521 tggcttatga cccccgcaaa aaagaatggg gcgtggccgt gcaatccaaa tttctggctt
     5581 gcgcggccgt cgtttcctgg gcgcgggcgg gagccggagc ggtcgccacg cagtctttcg
     5641 ccaatatcgc ctatgggatc gacggccttg atatgatgga gcgcaacatc agcgcggccg
     5701 acaccatcga ggcgctgacc accgccgacg agaaccgtgc ccagcgtcag gtgggtatgg
     5761 tggacaagaa tgggcgggcg ggcgccttca ccggcgacgg ttgctatgac tgggccggcc
     5821 acatcgttgg cgaggggttt acttgtcagg gaaatatact cgtgccgggc acggtcgagg
     5881 cgatggcggc tactttcgaa gcggcccgtc gcgggccagg tgagttggcc gattggctgg
     5941 ttgaggcgct ggcggctggg caagcggccg gtggcgatag tcgcggccgt caggcggccg
     6001 gcgtgatggt cgtgcgccac aacggcggct atggcggtaa taacgatcgt tatctggatc
     6061 tacgcgtgga cgatcacccc gagccgatcg agaagctggc cgaacttgtg accatgcatc
     6121 atctattgtt cggcgaggta gatcccgaca aactggtttc cctcgccggc gtggccggtg
     6181 atctgcaaaa agtgttgcgc cgaagcggcc acctgacgcg cgatgtcgat ggccagtttg
     6241 atgacgagac ccgtgaggcc ctgcgcgcgc tggtcggcag cgaaaacctc gaagaacgct
     6301 gggatggcac gggcgaaatg atcgacagcg ttgtcgtcga tttcctgatg cggcgctacg
     6361 gatagacagt tatgagtgaa gagagcaaga gagggttgct gatcgagcgg ccgatcgtcg
     6421 tgcgcaccag caattcgaaa tatgaaagcc gtcagactat actggctgca tgagagcgag
     6481 t
//
LOCUS       DAOMQG010000177         6394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5183_length_6394_cov_6.726140, whole genome shotgun sequence.
ACCESSION   DAOMQG010000177 DAOMQG010000000
VERSION     DAOMQG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5183_length_6394_cov_6.726140"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLC06_10265"
     CDS             complement(<1..481)
                     /locus_tag="PLC06_10265"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRO90871.1"
                     /translation="MTPLTIAIQAGGKSSRMGTDKSFVPFRGRPLIATVIDAVAGLGD
                     ELILITNKPDDYAHLGLPMFGDLYPETGPLGGIYTAIHAAAHPHVLTVACDMPWLNRP
                     LLAYMASLRETADVVVPRWEKYPEPLHAVYSKACLEPIREKLEAEMYKITVFYGRVRV
                     "
     gene            complement(566..1972)
                     /locus_tag="PLC06_10270"
     CDS             complement(566..1972)
                     /locus_tag="PLC06_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90872.1"
                     /translation="MMSKGQHRTQLLFLAILIALIVVAASEAVAGPTAPGANAGWEEI
                     GAGSASGGGISNTTGKSRFSNVAVGPDGMPVVAWLADRSGTYRVYVRRWDGTTWTEMG
                     SGSASDGGISNNVGDWQNPALAIGPDGMPVVAWAGGSNISDGDIYVRRWDGTTWAEIG
                     AGSASGGGISNGKGGSSPSVAIDHDGAPIVAWVGGDPNIDIYVRRWDGTAWVEIGAGS
                     ASGGGISNTSGWSGYPSLAVGPDGAPVVAWSDGSNDSDDTNNDEIYVRRWDGTTWVEL
                     GGSGSGGGISNTDNYSWYPTLAIDPDGMPIVAWDDRSSGNREIYVRRWDGTAWAEMGA
                     GSATGGGISHDPFDSQNPSLAIGPDGMPVVAWAKYGRNGAYAEVYVRRWDGAAWAEMD
                     AGSATGGGISNTKMDSWRPSLAIGPVGAPVVTWFDNSSDKYQIYVRRYRSVPGATFRA
                     FTPAVIFEAQPDAGASGD"
     gene            complement(2115..3314)
                     /locus_tag="PLC06_10275"
     CDS             complement(2115..3314)
                     /locus_tag="PLC06_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRO90873.1"
                     /translation="MATKNNRIRRLLLGLGAVILVGLAVAAFLFWRSTIPTAPSDFYT
                     PPDPLPAGEPGMIIRSEELTQNVPEGARAWRVLYLSRGADGQPAAVSGIIAAPTAASE
                     EPRPIVAWAHGTLGVQPACGTGHLDNPFEQIPQVARLMEQGYVLAATDYPGLGTPGVH
                     PYLFGEVEAAAVLDSVRAARQLDTGAGDRFAVWGHSQGGHATLWTAITAGTYAPELEL
                     VGAAAIAPAADLVGILNASMDKRAGAILVSMALAAWDANDPGASLEQLVAPDALAEVN
                     KLAELCLTTPSALLLLGELRPPVDYLPPNLTEIDAFQKFIAANTPDGPIDVPLIIAHG
                     TADSIIPFEGSVKEAVRRCAAGEDVELNRYPGDDHLPVGEHSAVGVIGWIEDRFAGRP
                     TGPNCGP"
     gene            3786..4217
                     /locus_tag="PLC06_10280"
     CDS             3786..4217
                     /locus_tag="PLC06_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRO90874.1"
                     /translation="MRSSLILLFVLLLGASLALAACGGGGETTTPAADEIDINALAPS
                     IDVQTAYAARSNPTVFMLDVREPDEYDAGHIPGITLIPMGEIPDRLSEIPTDVPVIVT
                     CRTGNRSGQVVDFLREQGFTNVHNMDGGIVAWQKAGYEVEE"
     gene            complement(4220..5065)
                     /locus_tag="PLC06_10285"
     CDS             complement(4220..5065)
                     /locus_tag="PLC06_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRO90875.1"
                     /translation="MQPCLTFVLGGGGARGAFQVGALRALYEAGLKPDLLVGTSIGAV
                     NAAAVALWGFDETGLYALELAYQKMSTAGLLDLNLSRLAMQALSGNLTFESGKRTAEF
                     LKSTGLPPELTFGDLTGPRLAVVGADLEIGRPVIYGLDPTESILSGVLASMSIPPWFA
                     PIQEDGHFILDGGALSNLAIEPSLGLGATEIYALDLEDPAAPAGGENMLVDLLRRLWF
                     AIGRRSLVLEMELAKARNVLVHHIPLNSEAATPVWDFSDYPALIRSGYEQAKEKLEEF
                     KIPDP"
     gene            5193..5441
                     /locus_tag="PLC06_10290"
     CDS             5193..5441
                     /locus_tag="PLC06_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO90876.1"
                     /translation="MSETDLLARITFNPQIYGGKPVIRGRRLAVEHVLGLLAAGDTSD
                     TLLAAYPWLEPDDIRACLLYARKLVAQERIEPLELASV"
     gene            5438..5791
                     /locus_tag="PLC06_10295"
     CDS             5438..5791
                     /locus_tag="PLC06_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRO90877.1"
                     /translation="MKVLLDTCVSGAVVLPALINAGHEVIWTGDWPNDPGDETILAFA
                     NDQERVPVTLDKDFGSLAVQHNRPHSGIVRLVNIATHDQAAVCLRVLADHGSILHSGA
                     IITAEWNRLRIRLPE"
     gene            5814..>6394
                     /locus_tag="PLC06_10300"
     CDS             5814..>6394
                     /locus_tag="PLC06_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRO90878.1"
                     /translation="MADKSLNKSASLDEAIWGHRWGYRDTYFVVNPDGSVRVTGDRYA
                     VSGYDMPGFIPFLEDTLAIKFDLNDTKPERADKPVPPPVVNAAFVAALAAELPAGHSS
                     DDPRERLLHSHGQTTVDEVYQVLYTHLDRVADLVVWPETHEEAAALVRLAAEYDVCLV
                     PYGGGTSVSAALKLPADETRMIVSVDMRRMDAI"
BASE COUNT         1150 a         2109 c         2034 g         1101 t
ORIGIN      
        1 gcacgcgcac gcgcccatag aagaccgtga tcttgtacat ttcggcctcc agcttctcgc
       61 ggatcggctc caggcaggcc ttgctgtaga cggcgtggag cggttcgggg tacttctccc
      121 agcgcggcac gacgacatcg gccgtctcgc gcagcgaggc catgtacgcc agcagcggcc
      181 gattgagcca gggcatgtcg caggccacgg tcagcacgtg gggatgggcg gcggcgtgga
      241 tggcggtgta gatgccgccc agcggccccg tctccgggta gagatcgccg aacatgggca
      301 ggcccaaatg ggcgtagtcg tcgggcttgt tggtgatgag gatcagctcg tcgcccagcc
      361 cggcgacggc gtcgatgacc gtggcgatga gcggccgccc gcgaaacggc acaaacgact
      421 tgtccgtgcc catgcggctg ctcttgcccc cggcctgaat ggcgatagtt aggggtgtca
      481 tggggagagt ataggggaga aattacgaat tacgaattac gaattacgaa ttcctcccgc
      541 aattcggccc cgccggccgc gccggtcaat ctcccgatgc gccggcgtcc ggctgggcct
      601 cgaagatcac cgccggggtg aaggcccgaa atgtcgcgcc gggcacggac cgatagcgcc
      661 ggacgtagat ctggtattta tcgctgctgt tatcgaacca ggtgacgacg ggcgcgccga
      721 cagggccgat ggccagagag ggtctccagg aatccatctt tgtgttgctg atgccgcccc
      781 ccgtggccga accggcgtcc atctccgccc aggccgcacc atcccagcgt cgaacataga
      841 cttcagcata ggcaccatta cggccgtact tggcccaggc gacgaccggc attccgtcgg
      901 ggccgattgc cagcgaggga ttttgagaat cgaaaggatc gtggctgatg ccgccgccgg
      961 tggccgagcc tgcgcccatc tccgcccagg ccgtgccgtc ccagcgtcgc acatagatct
     1021 cccggttgcc gctgctgcgg tcatcccagg cgacgatcgg catgccgtcg gggtcgatag
     1081 ccagcgtggg gtaccacgaa tagttgtcgg tgttgctgat gccgccgccg ctgcctgaac
     1141 cgcccagttc cacccaggtc gtgccgtccc aacgccggac gtagatttca tcgttgtttg
     1201 tatcatcgct gtcgttgctg ccatcgctcc aggcaacgac cggcgcaccg tcgggaccaa
     1261 cagccagcga ggggtacccg gaccacccgc tggtgttgct gatgccgccg ccggaggccg
     1321 agcctgcgcc tatctccacc caggctgtgc cgtcccagcg tcgcacatag atatctatat
     1381 tagggtcacc gcccacccag gcgacaatgg gcgcgccgtc gtggtcgatg gccacggagg
     1441 gggaactccc acccttgccg ttactgatgc cgccgccgga ggccgagcct gcgcctatct
     1501 ctgcccaggt tgtgccgtcc cagcgtcgca cgtagatatc cccatcgctg atattgctgc
     1561 cgccggccca ggcgacgacg ggcatgccgt cgggaccgat ggccagtgcg gggttctgcc
     1621 aatcgccgac gttgttgctg atgccgccgt cggaagccga gccgctgccc atctccgtcc
     1681 aggtcgtgcc gtcccagcgt cgcacgtaga cccgataggt gccgcttcta tcggccagcc
     1741 aggcgacgac gggcatgccg tcggggccga cagccacgtt cgaaaatcgg gacttgccgg
     1801 tcgtgttgct gataccgcca cccgatgccg accccgcacc gatctcttcc caaccggcat
     1861 tggcccccgg agcggtcggt ccggcgaccg cttcgctcgc ggcgacgacg attagtgcga
     1921 tcagaatcgc aagaaacagc agctgtgtcc tgtgttggcc tttactcatc atcggtttcc
     1981 tccccatatg attgtgactg gtttcaactg gtcttccggt ggctcgacca tcctcctgcc
     2041 ggcccaccgt tgtctgttca ggttatgcgc cggaataatg aaagccggca ccgggattat
     2101 ataccccttt cccttcacgg cccgcagttc ggcccggtcg gccgcccggc gaagcggtcc
     2161 tcgatccagc cgatgacgcc gacggccgaa tgttcgccaa cgggcaggtg gtcgtcgccg
     2221 gggtagcgat tgagttccac gtcctcgccg gcggcgcagc ggcgcaccgc ttccttcacg
     2281 ctgccctcga acgggatgat ggagtcggcc gtgccgtggg cgatgatgag cggcacgtcg
     2341 atggggccat cgggcgtgtt ggccgcgatg aacttctgga aggcgtcgat ctcggtcaga
     2401 ttgggcggca gatagtcgac gggcggccgc agttcgccga gcagcaggag ggccgagggg
     2461 gtcgtgaggc acagttcggc cagcttgttc acctcggcca gcgcgtcggg ggcgacgagc
     2521 tgttccagcg atgcgccggg gtcgttggcg tcccaggcag cgagggccat ggagacgagg
     2581 atggctccgg cccgcttgtc catcgacgcg ttgaggatgc cgaccagatc ggccgcgggc
     2641 gcaatggcgg ccgcgccgac cagctccagt tcgggcgcat aggttccggc cgtgatggcc
     2701 gtccagagcg tggcgtggcc gccctgcgaa tggccccaca cggcgaagcg gtcgcccgcg
     2761 ccggtgtcga gctgccgcgc ggcgcgaacc gagtcgagca cggccgcggc ttcgacctcg
     2821 ccgaagaggt aggggtggac gccgggcgtg cccaggccgg ggtaatcggt ggcggccagc
     2881 acgtagcctt gctccatcag tcgcgccacc tggggaatct gctcaaacgg gttatcgaga
     2941 tggccggtgc cgcaggcggg ctgcacgccc agcgtgccgt gggcccaggc gacgattggc
     3001 cgcggctctt cgctcgcggc ggtcggggcg gcgatgatgc cgctgacggc cgcgggctgc
     3061 ccgtccgctc cccgcgacag gtagagcact cgccacgccc gcgcgccttc gggcacattc
     3121 tgcgtcaact cctcgctgcg gatgatcatc cccggctcac ccgccggcaa gggatcgggc
     3181 ggggtgtaga agtccgacgg cgcggtcggg atggtggagc gccaaaacag gaaggcggcc
     3241 acggccagcc ccaccagaat aacggcccca agccccagta acagacgcct gatccggttg
     3301 tttttcgtcg ccatggataa cctcgccgtc ccgttcttga atcgcgatac aatggatgag
     3361 gtattttaga tggaaagggc tggatgagcc agtgaagggg gcgagcgaag gcgaccgacg
     3421 accacgcgac tcctgtcgca cggccacggc gacccctgtc gcacgaccac ggcgactcct
     3481 gtcgcacgac cacggcgact cctgtcgcgc ggccacggcg actcctgtcg cacgaccacg
     3541 gcgactcctg tcgcacgacc acggcaagag ggcgcttgaa gtacgaaaga ggccccggct
     3601 gtagcgcaag ccgccggctc gcagccacag gcaaagaggc aagccggcgg cttgcgctac
     3661 aggcagagag gtcgtaattt cttctcctct cgccgcgtcc atataccaaa tgccgcgcgg
     3721 ccgataaaag atggcctgtg cggatgctgt ctattaaact gggaagaaac cggagaacca
     3781 aaataatgag atcaagtttg atcctgctgt ttgtcctgtt gttgggcgcg tcattggcgt
     3841 tggccgcctg tggcggtggt ggcgagacga cgacccccgc ggccgatgag atcgacatca
     3901 acgccctcgc cccatccatc gacgtccaga ccgcctatgc cgcgcgaagc aaccccacgg
     3961 tcttcatgct cgacgtccgc gagccggacg aatacgacgc gggccacatc cccggcatca
     4021 ccctcatccc gatgggcgaa atccccgacc gcctgagcga aatccccacc gacgtgccgg
     4081 tcatcgtcac ctgccgcacc ggcaaccgca gcggccaggt cgtcgacttt ctgcgcgagc
     4141 agggcttcac caacgtccac aatatggatg gcggtatcgt cgcctggcaa aaggcgggct
     4201 atgaggtgga ggaatagaat tagggatcag gaattttgaa ttcttctaat ttctctttgg
     4261 cctgctcata gccgctgcgg atgagggcgg ggtagtcgga gaaatcccac acaggcgtgg
     4321 ccgcttcgct gttcagcgga atatggtgga cgagcacgtt cctggccttg gccagctcca
     4381 tctccagcac cagagagcgg cggccgatgg caaaccacaa ccgccgcagg agatcgacca
     4441 acatgttttc gccacctgcg ggcgcggcgg ggtcttccag atcgagggcg tagatctcgg
     4501 tcgcccccag gccaagagac ggctcaatgg ccagattgct cagcgcgccg ccgtcgagga
     4561 tgaaatggcc atcttcctgg ataggggcaa accagggcgg gatggacatc gaagccagca
     4621 caccggacag aatcgattcg gtcgggtcga ggccataaat gaccggccgg cctatttcca
     4681 gatcggcccc caccaccgcc agccgcggcc cggtcaggtc gccaaaagtt agctcggggg
     4741 gtaggccggt ggacttgagg aactcggccg tccgcttgcc gctttcgaag gtcaggttgc
     4801 cggagagcgc ttgcattgcc agccgcgaca gattcaggtc caggagaccg gccgtggaca
     4861 tcttctgata ggccagctcc agcgcataga ggcccgtctc gtcgaaaccc cacagggcga
     4921 cggccgccgc gttgaccgcg ccgatcgagg tgccgacgag caaatccggc ttgaggccgg
     4981 cctcatagag cgcccgcagc gcccccacct ggaacgcccc tcgcgctccg ccgccgccga
     5041 ggacgaatgt gagacagggt tgcatgagaa agagtataac gcaaaaacca caaatcgggg
     5101 tgggacagat attggaacgc ggatgacgcg gatcaggcgg atgcacaccg attatgatat
     5161 acttctcctg gagttaccgt tcggagcaaa ccatgagcga aactgacctg ctggcccgca
     5221 ttacattcaa cccacagatc tatggcggca agcctgtcat ccgtgggcgg cgactggctg
     5281 tggaacatgt cctgggcctg ctggccgccg gcgacacgtc ggatacgctc ctggctgctt
     5341 atccctggct tgaacccgac gacatccgcg cctgtctgct ctatgcccgc aagctggttg
     5401 ctcaggaacg gatcgaaccc ctcgaactcg cctcggtatg aaagtcctgc tcgatacttg
     5461 tgtgtcgggc gccgtcgtgc tgcccgccct tattaacgcc ggtcacgagg ttatctggac
     5521 cggcgactgg cccaacgacc ccggcgatga aacgatcctc gcatttgcca atgaccagga
     5581 acgcgtgccg gttaccttgg acaaggactt tggctcgcta gccgtgcagc ataaccggcc
     5641 ccacagcgga atcgtgcgat tggtcaacat cgcgacgcat gatcaggcgg ccgtttgctt
     5701 gcgcgttctg gctgatcatg gttctatcct gcactctggc gcaatcatca cggccgaatg
     5761 gaaccgcttg cgaatccgac tgcccgaata aatggagtaa atttcagaca attatggccg
     5821 acaaatccct caataaatcc gcctcgctcg acgaggctat ctggggccat cgctggggct
     5881 accgcgacac ctatttcgtc gtcaaccccg acggctccgt ccgcgtcacc ggcgaccgct
     5941 acgccgtctc cggctacgac atgcccggct tcatcccctt cctcgaggac accctcgcca
     6001 tcaagttcga cctgaacgat accaagccgg aacgggccga caagcccgtg ccgccgccgg
     6061 tggtcaatgc cgccttcgtc gccgcgctgg ccgccgaact gcccgccggc cacagcagcg
     6121 acgacccgcg cgagcggctg ctccacagcc acggccagac cacggtggac gaggtctatc
     6181 aggtgctcta cacccacctc gaccgcgtgg ccgacctcgt cgtctggccc gaaacccacg
     6241 aggaagcggc cgcattggtg cggctggcgg ccgagtatga cgtctgcctc gtcccctatg
     6301 gcggcggcac cagcgtcagc gcggcgctga agctgcccgc cgatgagacg cgcatgatcg
     6361 tctccgtcga catgcgccgc atggacgcca tcga
//
LOCUS       DAOMQG010000178         6366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5208_length_6366_cov_10.644906, whole genome shotgun sequence.
ACCESSION   DAOMQG010000178 DAOMQG010000000
VERSION     DAOMQG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_6366_cov_10.644906"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLC06_10305"
     CDS             complement(<1..307)
                     /locus_tag="PLC06_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRO90879.1"
                     /translation="MPTPTLRPDLQAVAALVPANARALDLGCGDGALLEHLKIEKGVQ
                     GRGIERGEAGVLACVRRGLSVRQGNLQEGLADYPDKSFDVVILSQTLQFLNDPMMILG
                     "
     gene            complement(407..1576)
                     /locus_tag="PLC06_10310"
     CDS             complement(407..1576)
                     /locus_tag="PLC06_10310"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRO90880.1"
                     /translation="MTHSIGIVIPQTFTFAEEEPFVLESGATLGPVTVAYETYGRLNA
                     DRSNAILILHALSGSAHAAGYHSAEDARPGWWDECIGPGKAFDTNRYFVICSNVLGSC
                     YGTSGPGSINPATGRPYGLNFPVVTIGDMVRVQVQLLDHLGIDRLLCAAGGSMGGMQV
                     LEWAAHHSHRLCAAIPLATTARHSPMLIALGEVGRQAIYADPNWQEGEYYGNGKKPDA
                     GLALARMVGHITYLSEASMHQKFGRRLETREQYGYEFQTEFAIESYLKYNGNNFTRRF
                     DANSYLYVTKAMDYFDLSRPTGSFAAAFAASAAIKFLIVSFTSDWLYPSYHSKELVRA
                     LHAVGADATYLDIESTWGHDAFLLEVDTMTRLLESFLDRLADEEGVAVPQEPVNQ"
     gene            complement(1651..2940)
                     /locus_tag="PLC06_10315"
     CDS             complement(1651..2940)
                     /locus_tag="PLC06_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRO90881.1"
                     /translation="MTTTNGRTFGFNTRQLHAGQVPDPTTGSRAVPIYQTTAYQFKNT
                     EHAANLFALKEFGNIYTRIMNPTTDVLEQRLADLEGGVGALAASSGHAAQTMAILTLC
                     GAGDHIVSSSHIYGGTYNQFNYTFPRLGIDVTFVDPAHPEAFEAAIRPNTKLIYGETL
                     GNPDISVFPFEEVAAIAREHKLPLVIDNTFASPYLCRPLEWGANIVVHSTTKFINGHG
                     TSIGGIIVDGGNFDWTSGRFDNFTTPDPSYHGLVYADLGAPAFILKARVQVLRDIGAS
                     QAPINSWLTIQGIETLSLRMDRHVANAQRVAEFLENHPQVGWVCYPGLKSHGDHERAK
                     KYLPKGPGAILGFGIQGGREAGEKFINNLKLFSHLANVGDARSLAIHPSSTTHSQLSD
                     AEQQSAGVTPDFVRLSIGLEDIEDILWDLDQALEASI"
     regulatory      complement(3119..3238)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3447..4625
                     /locus_tag="PLC06_10320"
     CDS             3447..4625
                     /locus_tag="PLC06_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRO90882.1"
                     /translation="MTSSLRQEFLLDPDVIFLNHGSFGATPRPVFESYQVWQRRLEWQ
                     PVQFLGTDIAAYLAEARQALGSYLNVAADDLVYVPNATFGVNVVARSLQLGPGDEVLA
                     TDHEYGACENAWLFMSRERGFRYARQPIPLPLASAADVVEQFWAGVTPQTKVIFLSHI
                     TSPTAMRLPVEAICARARAAGILTVIDGAHAPGQISLDLTAMGADFYTGNCHKWLCAP
                     KGAGFLFARPEVQHLVEPLIIGWGWGEGRTFTFGSDYLDYLQYPGTNDYAAYLAVPAA
                     IAFQAQHDWEVIRAGCRALVERAIGRINGLTGLPLLYPEPVGDAYSQMAIAALPDSID
                     LTVMKKRLIEEFRVEIPLIRWNGRQFIRISIQGYNDENDVDALLAALEIVLAGEAVGA
                     "
     gene            4664..5671
                     /locus_tag="PLC06_10325"
     CDS             4664..5671
                     /locus_tag="PLC06_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90883.1"
                     /translation="MAKRKGGFLDSLGDLIEKGIEELSREAYDEPAGGTPEPVKRRVS
                     LIIHNPRVPSAGNEHLDKVLRWNDTGRLIEGYISDLRECSGGYLNYEIVETITVDKFP
                     RKADGFTYTADGFVKAWNERKGFHDPDLVDYDALLEEFEMVRKVNENLVDEFWLFAFP
                     YAGYYESIMGGPGAFWCNAPPLTQTAHAKKRFIIMGFNYQRGVGEMLEAFGHRAESIM
                     KYTFRRERGDDNLWERFIRHEFKNPGQSEVGWMHYAPNSERDYDWGNKREVLSRWRTW
                     RNFPNLAGEAEMVDCHDWGDGDIRLHHKWWFELLPKIGGSANGIAYNWWQYIVDPNLV
                     R"
     gene            5690..6172
                     /locus_tag="PLC06_10330"
     CDS             5690..6172
                     /locus_tag="PLC06_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90884.1"
                     /translation="MKLTLTRVLLIAILVLGVLLFFLLVNDAVSPGHDQEEPTQGPTI
                     ESPAEPGGATQCPVATPEFFNVEPVTSPTDKLSQIVTVDLGQGETITITTESGIFTAP
                     FDAFPTEIEIELLPNTTHNLTVEGRVREVVQGECTYGGYTLSTTRDRYGDPLVIEQKQ
                     "
BASE COUNT         1206 a         1797 c         2062 g         1301 t
ORIGIN      
        1 cgcccaggat catcatcggg tcgttcagga actggagcgt ctggctgagg atgacgacat
       61 cgaagctctt gtcggggtaa tcggccaggc cctcttgcag gtttccctgc cggacgctga
      121 ggccgcgccg gacacaagcc aggacgcccg cctcgccacg ctcgatgccg cgcccctgca
      181 cccccttttc gatcttcaga tgctccagca acgcgccgtc gccgcaaccg aggtcgagtg
      241 cgcgcgcgtt cgccgggacg agtgcggcta cggcttgcag atcagggcga agggtggggg
      301 tgggcatgtt ggtcagtggg tcagtaagac agtaattcag taagacagta ggtcagtgga
      361 tcagtaggtc agtggggcaa tgggaactga atactgacct gctgacctac tgattcaccg
      421 gctcctgggg cacggcgacc ccttcctcat cggcaagccg gtcgagaaag ctctccaaca
      481 gccgggtcat ggtatcgact tccagcagga acgcatcatg gccccacgtg ctttcgatgt
      541 cgagataggt cgcatcggct cccacggcgt ggagcgcccg caccaactcc ttgctgtggt
      601 aggatggata gagccaatcg ctggtgaagc tgacgatgag gaacttgatc gccgccgacg
      661 ccgcaaacgc ggcggcgaac gaacctgtcg gccggcttag atcgaagtaa tccatggcct
      721 tggtcacata gaggtagctg ttggcgtcga agcggcgcgt gaagttgttg ccgttgtatt
      781 tcaggtagct ctcgatggcg aattcggtct ggaactcgta gccgtactgc tcgcgagtct
      841 ccaggcggcg accgaatttt tggtgcatcg acgcttcgct gaggtaggtg atgtgcccga
      901 ccatgcgcgc caatgccaac ccggcgtcgg gctttttgcc attgccgtag tattctcctt
      961 cctgccagtt cgggtcggcg tagatcgcct gccggccgac ttcccccaga gcgatcagca
     1021 tcgggctgtg gcgggctgtt gtcgccaacg ggatggccgc gcacagccgg tgggaatgat
     1081 gcgcggccca ctccagcacc tgcatgccgc ccatcgagcc gccggccgcg cagagcagcc
     1141 ggtcgatgcc cagatgatcc agcagctgca cctggacgcg aaccatgtcg ccgatggtga
     1201 cgacggggaa gtttaagccg tagggacggc ctgtggccgg gttgatgctg ccggggccac
     1261 tggtaccgta gcagctgccg agaacattgc tgcagatgac gaagtagcga ttggtgtcga
     1321 acgccttgcc ggggccgatg cactcatccc accagccggg gcgggcatct tcggccgagt
     1381 gatagcccgc agcgtgagcg ctgccgctga gcgcgtgcag gatgaggata gcgttgctgc
     1441 ggtcggcgtt caggcggccg tacgtttcgt aggccaccgt caccgggccg agcgtcgcgc
     1501 cgctctcaag gacgaacggc tcctcttcag caaaggtgaa agtctgcgga atgacgatgc
     1561 caatcgagtg agtcatgatc acaattgaga gtgacgaatg acgaatgggt ttcattcacc
     1621 cgttcgtcac tcgtcacccg tcgctcgtaa ctagatggat gcttccagcg cctgatccag
     1681 gtcccagagg atatcctcga tgtcttcgag accgatgctg aggcgcacga agtcgggggt
     1741 gacgccggcc gactgctgtt ctgcgtcgga gagttggctg tgggtcgtgc tggatgggtg
     1801 gatggccaga ctgcgggcgt cgccgacgtt ggccagatgg ctgaacagct tcaggttgtt
     1861 gatgaacttc tcacctgctt cgcggccgcc ctggatgccg aagcccagga tcgcccccgg
     1921 tcccttgggc aggtactttt tggctcgctc gtggtcgccg tggctcttca ggccgggata
     1981 gcagacccag cccacttgcg ggtgattttc caggaactcg gctacgcgct gggcgttggc
     2041 cacatggcga tccatgcgca ggctcaacgt ctcgataccc tggatggtca gccagctatt
     2101 gatcggcgcc tggctggcgc cgatgtcgcg cagaacctgc acccgagcct tgaggatgaa
     2161 agccggcgcg cccaggtcgg catacaccag accatggtag gaaggatcgg gcgtggtgaa
     2221 gttgtcgaag cggccgctgg tccagtcgaa attgccgccg tcgacgatga tgccaccgat
     2281 cgatgtgcca tggccgttga tgaatttggt cgtgctgtgg acgacgatat tggcgcccca
     2341 ctcaagcgga cggcacagat agggcgaagc aaaagtattg tcgatgacca gcggcagctt
     2401 atgctcccgg gcgatggccg ctacttcctc gaacgggaag acgctgatgt cgggattgcc
     2461 caatgtctcg ccgtagatga gcttggtgtt ggggcggatg gccgcctcga atgcttccgg
     2521 gtgcgccgga tcaacgaagg tgacgtcgat gccgaggcgc gggaacgtgt agttgaactg
     2581 gttatacgtg ccaccataga tatggctgct gctgacgatg tggtcgcctg cgccgcacaa
     2641 ggtcaggatg gccatcgttt gcgcggcgtg gccgctgctg gccgccaggg cgccgacgcc
     2701 gccttccaga tcggccagtc gctgctccag cacgtcggtc gtcgggttca tgatgcgggt
     2761 gtagatattg ccgaactcct tcagcgcaaa cagattggcc gcgtgctccg tgtttttgaa
     2821 ctgataggcg gtggtctggt agatgggcac ggcccggctg ccggtggtcg ggtcgggaac
     2881 ttgtcccgca tgcagctgcc gcgtgttgaa tccgaaagtg cgtccgttag tcgtcgtcat
     2941 gtgaatcatt ctccttctgt tgatattttc gtctcaactg cggtcatctg tgctgcgcgg
     3001 tcaatcctga ctgcgttgaa ggaggatcaa aaagaccctc gatggagagt ccagagggtc
     3061 ggcgtggcag gtgttctgtt ggtatgtcgg cgtggcggga ttgcgcgtgt ccctgtcgct
     3121 ctcatcttcc agaatcgtct gccggaattg gcaccctgtt gactgaacga atccctgccg
     3181 ttctatccgg gttgccgagg tttcttaggg ccggtccctc cacctctctg gataaaagtg
     3241 acggtatgca gttgttggag ggaactataa cggacggttt gggggttgtc aagaggattg
     3301 acaagcaaaa acatgatgaa tatcggcaat aaccatggca gatacctgca aagcgcctga
     3361 tttgccatta agagggctgg ggtggacaat tctccggcga cctgttcggt cgtaatcatc
     3421 aatgctattt atctgaatta atcgcaatga cctcatcact tcgtcaagaa ttcctgctcg
     3481 accccgacgt tatttttctc aatcacggct ccttcggggc cacaccgcgg ccggttttcg
     3541 agtcgtacca ggtgtggcag cgacggctgg aatggcagcc agtacagttt ctgggaaccg
     3601 acatcgcggc gtatctggcc gaggcgaggc aggcgctggg tagctacctc aacgtggccg
     3661 ccgatgatct ggtctatgtg cccaatgcca cgtttggcgt caacgtcgtg gctcgctcgc
     3721 tgcagttggg gccgggcgac gaggtgctgg cgaccgacca cgaatatggc gcgtgcgaga
     3781 acgcctggct gttcatgagc cgagagcgcg gctttcgcta cgcccggcag ccgattcctt
     3841 taccgttggc ttccgcggcc gatgtcgtgg aacagttctg ggcgggcgtg acgccgcaga
     3901 cgaaagtaat tttcctgagc cacatcacct cgcccacagc gatgcgtttg cccgtggagg
     3961 ctatttgcgc gcgggcacga gcggcgggca tcctcaccgt catcgatggg gcgcatgcgc
     4021 cgggccagat atcgctcgat ctgacggcca tgggggccga cttctacacc ggcaactgcc
     4081 acaaatggct ctgtgccccg aagggggcgg ggttcctttt cgcacggccg gaagttcagc
     4141 atctggtcga accgctgatc atcggctggg gttggggcga ggggcggacg tttaccttcg
     4201 gcagcgacta tctggactat ctccaatatc ccgggaccaa tgattacgcc gcctaccttg
     4261 ccgtcccggc ggcgatcgct ttccaggcgc agcatgattg ggaagtcatt cgcgccggct
     4321 gccgggcgct ggtggaacgg gcgatcggcc gcatcaatgg cctgaccggc ttgccgttgc
     4381 tctatcccga accggtcggc gacgcataca gccagatggc gattgctgcc ctgcccgatt
     4441 ccatcgacct gacagtgatg aaaaagcgcc tcattgaaga atttcgcgtg gagatccctc
     4501 tgattcgctg gaacggccgg caattcatcc gcatctcgat tcaggggtat aatgacgaga
     4561 acgacgtcga tgcattgctg gcggctctgg agatagtgtt ggccggcgag gccgtcggcg
     4621 cataaaccgg attaggtttc atccctgaca ggagaggatg atcatggcaa agcgaaaagg
     4681 cgggtttctg gacagccttg gcgatctgat tgagaagggt attgaggagt tgagccggga
     4741 ggcttatgat gagccggccg gtggtacgcc ggagccggtt aagcggcgcg tgtcgctcat
     4801 catccacaac ccgcgcgtgc cgagcgccgg taatgagcat ctggacaagg tgctgcgctg
     4861 gaacgatacc ggccgattga tcgaaggcta cattagcgac ctgcgcgaat gcagcggcgg
     4921 ctatctgaac tatgagatcg tcgagactat caccgtcgat aagttcccgc gcaaggccga
     4981 cggcttcacc tacaccgccg acgggttcgt caaggcctgg aacgagcgca aggggttcca
     5041 cgatcctgat ctggtcgatt acgacgcgct gctcgaggag ttcgagatgg tgcgcaaggt
     5101 gaatgaaaac cttgtcgatg agttctggct gtttgccttc ccctacgccg ggtattacga
     5161 atcgatcatg ggcgggccgg gggcgttctg gtgcaatgcg ccgccgctga cgcagactgc
     5221 tcatgccaag aagcgcttca tcatcatggg cttcaactat cagcgtggcg tcggcgagat
     5281 gctggaggca ttcggccatc gggctgagtc gatcatgaaa tataccttcc gccgtgagcg
     5341 gggagacgac aatctgtggg agcggttcat tcgccacgaa ttcaagaacc ccggccaatc
     5401 cgaggtcggc tggatgcatt acgcgcccaa cagcgagcgc gattatgact ggggcaacaa
     5461 acgcgaggtg ctgagccgct ggcgcacgtg gcgcaatttt cccaacctgg cgggcgaggc
     5521 ggagatggtt gattgtcacg actggggcga cggcgacatc cggctgcatc ataaatggtg
     5581 gtttgaactg ctgccgaaga taggcggctc ggccaacggg atcgcctaca actggtggca
     5641 atacatcgtc gatcccaatc tggtgcgtta gttcacagga ggtggcggga tgaaactgac
     5701 tctcacgcgc gtgctgctca tcgccattct ggtcctgggg gtccttctct ttttcctgct
     5761 ggtcaacgat gccgtgtcgc cgggtcatga tcaggaggaa ccgacgcaag ggccgacgat
     5821 cgaatcgccg gcagagcctg gtggagcgac ccaatgcccc gtggccacgc cggagttctt
     5881 caacgtggag ccggtcacat caccgaccga caagctgagt cagatcgtca ccgttgatct
     5941 gggtcagggc gagacgatca cgatcaccac cgaatcgggc atattcaccg ctccgtttga
     6001 tgccttcccc acggaaatcg agatcgagct gctgccgaac acgactcata acctgacggt
     6061 cgaagggcgc gtgcgcgaag tggtgcaggg agagtgcacc tatggcggct acaccctgag
     6121 cacgacccgc gaccggtatg gggatccgct ggtgatcgag cagaagcaat agaggatgga
     6181 caagccaaac atggaactga ctgaacgcga ccgggcgatg ctggccggcg agatgggcga
     6241 ggcgacgcaa atggccatgc gcatcctggt gacgatggcc ggggtctatg gcgcggagcg
     6301 cctgctcgat atcgaatcgg cccacatcga cggctgtctc tatcacggct attccgggct
     6361 ggagtt
//
LOCUS       DAOMQG010000179         6349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5222_length_6349_cov_8.810931, whole genome shotgun sequence.
ACCESSION   DAOMQG010000179 DAOMQG010000000
VERSION     DAOMQG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_6349_cov_8.810931"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..432)
                     /locus_tag="PLC06_10335"
     CDS             complement(43..432)
                     /locus_tag="PLC06_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90885.1"
                     /translation="MSMLVTIHGELRWLVVLAAVVVIVKFLMGWLGKRQYASLDQKLL
                     LVFTILLDINVLLGLIILFFGGGFSGPRLEHATTMILAVIAAHMTAMWKRSTNDSLKF
                     RNQLLMVVLAVILVIFGVIRLRGGFMF"
     gene            complement(481..1929)
                     /locus_tag="PLC06_10340"
     CDS             complement(481..1929)
                     /locus_tag="PLC06_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRO90886.1"
                     /translation="MSRYLLFGLALPGIGLFFAVAPAAGSLPSPSGLLLPAIVIVALV
                     LINAVFVAAEFAIAGVRDTELEQLADEGNRAAGNMLRMLESQPRLDHYVATAQLIVTL
                     ASLSLAMYGVPIIARFAAPYLLATGLEPGIATWAAYLIAILILAYLHVVIGEMAPRAL
                     AISNPAGTALALDRIMRLAGRLFGLPVRVLNGIGGHLLRAFRITSAQGQPRLMAAEEL
                     ELIVTESAEGGLIEDDEQEIILNIFDFSERTVGQVMTPRRKVQAIPIDCEERTLIRTV
                     TESRHSRFPVYDGDLDHIAGILHLKDLIRLTLGPEGPFDLRLILRTAPDIPEDFQVDR
                     LLAAFKRDKLHMAIVRDEFGGFSGVVTLEDLVEEVVGEVRDEFDVEREPYDKVGPGVL
                     EVSGDYLLDYLSDDVDLGDEDDLPNVETVGGLIVALLGRPPINGDIVTFRDNIRLTVL
                     DTDRLAVARARVEFPSPGKPADLSEDENQEEE"
     gene            complement(1922..3286)
                     /locus_tag="PLC06_10345"
     CDS             complement(1922..3286)
                     /locus_tag="PLC06_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRO90887.1"
                     /translation="MMVLTSIAVIGLALFLAGLYVAAEFAIGAAHRTKIGQPAAQNDS
                     RARMILRILEDNNRLNSYIAACQIGITIATLALGAFAQVYVAGLLAGPMAQLFAGWIS
                     PLAAARFGHALAVLGVLLLIGGMQVILGELFPKSVALQYPEAIALAVAWPMRLSLILF
                     ALPVWLFRATTRAILRLLGLKEEDFVGRARSVEEIEELVTTSHEGGLLDDNERRLLRN
                     ALRMRDLTARQVMVHRTRIASAPVDVTPLELMQMAIDAGFSRIPIHGDSIDDIVGFVH
                     VKDVFRLYNEGVSDIRSVIRDVVYVPETLSINDVWDQLNRQNRYLAIVFDEFGGTAGL
                     LTLEDVIEEIFGELQDEFDDEAAVIARDKEGRIYLRGDLLIADVNEYLDLDLPEESAD
                     TLSGLVFSLLGRPPVVGDEVRSEDVLIRVETMEDLGVGEVSLLLPPGDEESPFTEWDI
                     ADYE"
     gene            complement(3638..6286)
                     /locus_tag="PLC06_10350"
     CDS             complement(3638..6286)
                     /locus_tag="PLC06_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRO90888.1"
                     /translation="MTKQPAYTRIDFAPVADPDAVVTAGNARFTVLGPRLIRMEYAPD
                     GCFEDRPSQAFWYRRQPVPRYVVTRDDSGLVLTTDYLRLEYAGQGRPFAADSLRVTLL
                     PDGPVWHYGDPAPGNLLGTARTLDQVSGSMKLEPGLLSRDGWTVIDDSRSLAFGEAKW
                     LTARDSADGSHDLYFFGYGQAYHEALRAYLSVTGDVPLLPRWALGNWWSRYWEYSDGD
                     LRALMTDFREHDVPLGVCIVDMDWHLVNVGEGINGWTGYTWNPDLFPDPAGFIRWLHD
                     QGLRTALNLHPALGVRPHEAQYPAMAERLGLDPGSDQPIPFDIAGREFTDAYFEVLHH
                     PYEADGVDFWWMDWQQGTRSAVFGLDPLWWLNHLHFHDLGRDGKRPFTFSRWGGLGNH
                     RYPIGFSGDTVVSWESLAFQPYFTATAANVAYGWWSHDIGGHMQGIEDRELYTRWVQF
                     GVFSPIFRLHSTKNPFHDRRPWGYDAEVLQITRDAMQLRHALIPYLYTMSRLNELEGR
                     SLVRPMYHDYPDREEAYACPQQYLFGTDLIVAPYTEPADPDTRLSRQAFWLPPGDWYH
                     LFTGEYYRGDAWYARYGGLDDVPVFARAGAILPLGPKVGWGGLDNPEELHLHVFAGQD
                     GQFSLYEDEGDGVAYRAGAYAQSRFEQRWDDSSLRFTIFPPSGDTTLIPQEREHHLHF
                     YGVAEPGQVTVMIDGATRAVNWQYDAEREVIHAESLRLAPGAQARITILAADGSPLLS
                     RRDRTRETVQAMIGAFRLDSLAKAWTVERLDGLIAGPESLADFGMELTTAQARALLEV
                     TRGAGAHWVRDIGEPDLIVLWNNRESAGFRHYFAELRPEKWFAAERYGSSIGVVPRFR
                     AIRPSGERWRLSVDYFGLQSLTFHGN"
BASE COUNT         1385 a         2076 c         1833 g         1055 t
ORIGIN      
        1 cgaggcgcag gcatcccgtg ggacgcccgc gcctcgtttt gtttagaaca taaacccgcc
       61 gcgcaggcgg atcacgccga agatgaccag aatgacggcc agcacgacca tcagcaactg
      121 attgcgaaac ttgagcgagt cattggttga tcgtttccac atcgcggtca tgtgcgccgc
      181 gatgacggcc aggatcatcg tcgtcgcatg ctccagacgc ggcccgctga atccgccgcc
      241 gaagaacagg atgatcaacc ccagcagcac gttgatgtcg agcagaatcg tgaagaccag
      301 caagagcttt tgatcgagtg aagcatattg gcgcttgccg agccagccca tcaggaactt
      361 gacgatgacg acgacggccg ccaatacgac cagccatctc aactcgccgt ggatggtcac
      421 taacatactc atattgatta ctcctttaac ttatgtgtga gcggcaaact cgccgctcgc
      481 tcattcttct tcctggttct cgtcttccga caggtcggca ggcttgcccg gtgacgggaa
      541 ttccacccgc gcgcgggcga ccgccagccg atcggtgtcc aggacagtca agcgaatatt
      601 atcgcgaaaa gtgacgatgt cgccattgat gggcggccgc cccagcaagg cgacgatcag
      661 cccgcccacc gtttccacgt tgggcaggtc gtcctcatca cccaaatcga catcatccga
      721 cagataatcg agcagataat cgcccgacac ttccagcacg ccgggaccga ctttatcata
      781 cggctcgcgc tcaacgtcga actcgtcgcg cacctcgccg acgacctcct cgaccagatc
      841 ttccagcgtc acgaccccgg aaaacccgcc gaactcatcg cggacgatcg ccatatgcag
      901 cttgtctctc ttgaaggccg ccagcaaacg atcgacctgg aaatcttcgg gaatgtccgg
      961 cgcggtgcgc aggatgagcc gcagatcgaa cgggccttcc ggccccaggg tcagtcgaat
     1021 caagtccttc aggtgcagaa tgccggcgat atggtccaga tcgccgtcat agaccgggaa
     1081 gcggctgtgg cggctctccg tcaccgtccg gatgagggtt cgttcctcgc aatcgatggg
     1141 gatcgcctgc accttgcgcc gcggcgtcat cacctggccg accgtgcgct cgctgaagtc
     1201 aaagatgttg aggatgatct cctgctcgtc gtcctcaatg aggccccctt cggcgctctc
     1261 ggtgacgatc agctccagct cctcggccgc catcaggcgc ggctgtccct gggcggaagt
     1321 aatgcgaaat gcccgcaaca gatgcccgcc aatgccgttc aggacgcgaa ccggcaagcc
     1381 aaacagccgc ccggccaggc gcatgatccg gtcgagcgcc aacgccgtgc ccgcggggtt
     1441 ggagatagcc agcgctcgcg gcgccatctc accgatgacg acgtgaaggt aagccagtat
     1501 caggatggcg atcaggtaag cggcccaggt ggcgatgccc ggctccagcc ctgtggccaa
     1561 cagatatggc gcggcaaacc gcgcgatgat cggcacgcca tacatggcca ggctcaggga
     1621 agccagggtc acgatgagtt gggcggtcgc cacgtaatgg tcgagtcgtg gctgcgactc
     1681 cagcattcgc aacatgttcc cggccgcccg gttgccctcg tcggccagtt gctccagttc
     1741 cgtgtcccga acgccggcaa tggcaaattc cgcggccaca aacacggcgt tgatcagaac
     1801 cagcgccacg atcacgatgg cgggcagcag caagccggac ggagacggca aggacccggc
     1861 cgcgggagca acagcgaaga acaggccaat gcccggcaac gccaggccga agagaagata
     1921 cctactcata atcggcgata tcccactccg tgaatggcga ctcttcatcg ccgggcggca
     1981 gcaataacga gacctcgccc actcccaaat cctccatcgt ctcgacgcga atcagcacat
     2041 cctcgctccg aacttcatcc cccacgaccg gcggccgccc cagcaagcta aacacaagcc
     2101 cgctcagcgt gtcggccgat tcctcgggca ggtcgaggtc gagatattca ttcacgtcgg
     2161 cgatcagcag gtcgccgcgc agatagatgc gcccctcttt atcgcgggcg atgaccgccg
     2221 cctcatcatc gaactcgtcc tgcagctcgc cgaatatctc ttcgatcacg tcttccagcg
     2281 tcagcaggcc cgccgtcccg ccaaactcat caaagacgat cgccagatag cggttctgcc
     2341 ggttcaactg atcccacaca tcgttgatcg acaacgtctc gggcacataa acgacgtcgc
     2401 ggatcacgct gcgaatatcg gaaaccccct cgttatacag gcgaaataca tccttcacat
     2461 ggacgaagcc gacgatatcg tcgatcgaat caccatggat agggatacgc gagaacccgg
     2521 cgtcgatggc catctgcatc agttcaagcg gtgtgacgtc aacgggcgcg ctcgcaattc
     2581 gggttcgatg caccatcacc tgccgcgccg tcagatcgcg catccgaagc gcgttgcgca
     2641 acaaacgccg ctcattatca tccagcaggc ccccctcatg gctggtcgtc accagctcct
     2701 caatctcttc gaccgagcga gcgcggccga caaaatcttc ctctttcagg cccaacagcc
     2761 gcagaatagc gcgagttgtg gcccggaaca gccacacggg caaagcgaac aggatgagcg
     2821 agaggcgcat gggccaggca acggcgagag cgatcgcttc aggatactgc aaggccaccg
     2881 acttggggaa tagctccccc aggatgacct gcatgccgcc gatcaacaag agcacgccga
     2941 ggacggccaa tgcatgaccg aaccgggcgg cggcaagagg gctgatccag cccgcgaaga
     3001 gctgtgccat cggccccgcc aacagaccgg cgacataaac ctgcgcgaac gcgcccaggg
     3061 cgagggtcgc aatcgttatc ccgatctggc aggcggcgat gtagctgttc agccggttgt
     3121 tatcttccag gatgcgcagg atcatccgcg cgcgcgagtc attctgcgcg gccggctggc
     3181 cgattttcgt tcgatgagcc gccccaatgg caaattccgc cgccacatac aaccccgcca
     3241 aaaagagagc caggccaatg acggcgatgg acgtcaaaac catcatggcg tgaagtcctc
     3301 ttcaatcatg atggctgatc ggccgttcgc cctcgcaatc gtcaaagagg cgaagcagca
     3361 ggtcaacaaa gcagtcgacg gatggcgcgt cactggccaa cagaccggca tgtgctggac
     3421 aacaggtgtg gggggctgac tcggaggctc cgggacgggg gcagacaagg ttaatgcact
     3481 ccccggtatt caggccatgt cgccgcctgg cgcgcggatg cgaaagaagg tttcactttg
     3541 gtcaatttcg atgagttact cacgctccaa caggtcagag cgaaggacaa ttaccgcccg
     3601 aattatagtg agtccaccct gaaaaatcaa tcggggttca gttgccgtga aatgtcaggg
     3661 attgcaggcc aaaataatcc accgacagcc gccaacgttc gcccgacggc cgaatagccc
     3721 gaaagcgcgg cacgacccca atcgacgacc catatcgctc ggccgcaaac cacttctccg
     3781 gccgcaactc cgcaaaatag tgccgaaacc cggctgattc ccgattgttc cacagcacga
     3841 tcaggtccgg ctcgccgata tccctgaccc aatgcgcccc cgccccccgc gtcacttcca
     3901 gcaacgcccg cgcctgcgcc gtcgtcagtt ccatgccgaa atcggccagg ctctccgggc
     3961 cggcgatcag cccgtccagc cgctccacgg tccacgcctt ggccagcgaa tccagacgaa
     4021 acgccccgat catcgcctgc accgtctccc gcgtgcggtc tcggcgggac agaagcggcg
     4081 agccatcggc cgcaaggatg gtgattcgcg cctgggcgcc gggagcaagt cgcagggatt
     4141 ctgcgtgaat gacctcgcgc tcggcgtcat attgccaatt cactgcccgc gtggccccat
     4201 cgatcatgac cgtcacttgt cccggctcgg ccacaccata gaaatggagg tgatgctccc
     4261 gttcctgggg gatcaatgtc gtgtccccgc tgggcgggaa gatagtgaat cgcaacgagg
     4321 aatcatccca acgctgctca aagcgactct gggcataagc gccggcgcgg taggcaacgc
     4381 catcgccctc atcctcataa agggagaact ggccgtcctg cccggcgaac acgtgcaaat
     4441 gcagttcttc gggattatcc aggccgcccc aacccacctt cgggcctaac ggcaggatcg
     4501 ctccggcgcg ggcgaaaacg gggacatcat ccagcccgcc gtaccgcgca taccaggcgt
     4561 cgccgcggta atattcgccg gtgaagagat gataccaatc gcccggcggc agccagaacg
     4621 cctggcggga cagccgcgtg tcggggtcag ccggttcggt ataaggcgcg acgatcagat
     4681 cggtcccgaa cagatattgc tgcgggcaag cgtaggcttc ctcacgatcc gggtagtcgt
     4741 ggtacatggg ccgaacgagc gaccggcctt ccagttcgtt caggcgcgac atcgtgtaga
     4801 ggtaggggat caacgcatgg cgcagttgca tggcgtcgcg tgtgatctgc agcacctcgg
     4861 cgtcataccc ccacggccgc cggtcatgaa aagggttctt ggtgctgtgc agccgaaaga
     4921 tggggctgaa cacgccgaac tgcacccacc gcgtatatag ctcgcggtct tcgatgccct
     4981 gcatatgccc gccgatatca tggctccacc agccataggc gacgttggcc gccgtcgccg
     5041 tgaaatacgg ctggaacgcg agcgactccc acgagacgac cgtatcgccg gaaaagccga
     5101 ttgggtagcg atgattgccc aacccgcccc aacgggagaa agtgaacggc cgcttgccgt
     5161 cccgccccag atcatggaaa tgcaggtgat tgagccacca cagcgggtcc agtccgaaaa
     5221 cggccgaccg ggtgccttgc tgccaatcca tccaccagaa atccacgccg tcggcttcat
     5281 aggggtgatg aagaacctcg aagtaggcgt cggtgaactc ccggccggca atatcaaacg
     5341 ggatcggttg gtccgaaccg ggatcaagcc ccaaccgctc ggccatggcg ggatactgcg
     5401 cctcatgcgg ccgaacgcca agcgccggat gcagattcag cgcggttcgc agcccttgat
     5461 cgtggagcca gcgaataaag ccggccgggt ccggaaacag gtcagggttc cacgtgtagc
     5521 ccgtccagcc gttgatgccc tcgcccacat tgaccagatg ccagtccatg tcaacgatgc
     5581 acacccccag cgggacgtca tgctcgcgga agtccgtcat cagggcgcgc aagtcgccgt
     5641 cgctgtactc ccagtagcgg ctccaccaat tgcccagcgc ccaccgtggc agcaggggaa
     5701 catcgcccgt gaccgagaga taggcgcgca aagcctcgtg atacgcctgc ccatatccga
     5761 aaaaatagag atcgtgggat ccatcagccg aatcgcgcgc cgtcagccac ttcgcctcgc
     5821 cgaaggccag gcttcggcta tcgtcgatga ccgtccagcc gtcgcgcgac aagaggcccg
     5881 gctccagctt catgctgccg ctcacctgat cgagcgttcg cgccgtgccc aacaggttgc
     5941 ccggcgccgg atcgccgtag tgccacacag ggccgtccgg taacagagta acacgcaacg
     6001 aatcggccgc gaacggccgc ccctgcccgg catattcaag gcgcaaatag tcggtcgtca
     6061 ggacaaggcc gctatcgtcg cgcgtgacca catatcgcgg cacaggttgg cggcgatacc
     6121 agaacgcctg actgggtcga tcctcgaagc agccatcggg cgcatactcc atgcgaatca
     6181 ggcgtgggcc aaggacggta aagcgtgcat tcccggccgt cacaacggca tcgggatcgg
     6241 caacgggagc gaaatcgata cgagtataag caggttgctt ggtcaatgaa tgccctcaaa
     6301 ataaatcttg ccggcccaat gccggaaagc gaattaattt caatgggag
//
LOCUS       DAOMQG010000180         6339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5233_length_6339_cov_6.585933, whole genome shotgun sequence.
ACCESSION   DAOMQG010000180 DAOMQG010000000
VERSION     DAOMQG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5233_length_6339_cov_6.585933"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLC06_10355"
     CDS             <1..251
                     /locus_tag="PLC06_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90889.1"
                     /translation="GVSIEAGTGLEMQSIAAAVLGGTMLLGGRGSVPAAMTGALALEV
                     LFTLLNLMGLPKPLRDAVQGLIIIGAVAYTAYSTRKTR"
     gene            429..1700
                     /locus_tag="PLC06_10360"
     CDS             429..1700
                     /locus_tag="PLC06_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018469832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO90890.1"
                     /translation="MKPLRVLILMVAMMLMIALVACGGGAATTQAPAATEPAAEAPAA
                     EEPAAEEPAAEEPAASTGAGLGMEAAAVAEQFFSQADLDRSIALLDATPMGPEGQPWV
                     QYLEEHFVDTSQYAKEGPYTFCFSNAGVNNPWRVVGYNTMTAEVEMHPEIAEWIHVDA
                     EGSDEKQISDIQDLVAGGNCDILIVSPNTTQALTPAVEEACKSLPVVVFDRGVQTDCP
                     ATFVQPIGGYAFGYVGAKFLADNLEPGAKVVALRILPGVDVLETRWAAAKQVFEQEGI
                     DVVEVAFGDGNNDKVKTIITDAIQKYGQIDGVWMDAGATAVAAIEAFEDAGAPYPVIN
                     GEDQQDFLVKWQQEGLTAIAPTFPTFQWRSAVIAALMVLQGEQVNDPWILPQPTVTQE
                     NLDQYVQPDMPPLFYAMCGCEGMPGFPERWQ"
     gene            1921..2787
                     /locus_tag="PLC06_10365"
     CDS             1921..2787
                     /locus_tag="PLC06_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRO90891.1"
                     /translation="MIEIGFHTDAFNSAYWSFERCLQWAQDNDVHYIECGTIDGVSWI
                     HGLGYQPHVALWEDPLALREKMARYGVRFSQLDAAFPLSLPTATSLGETYIRNSIRWA
                     YLAGCDHIDTTDNMHRPEGLSDEEALEMMKRIYGNVLEDAARYGMTINIEPHGYYTTK
                     PDYMARMLDFFDTPHLRMNMDTGNTFIAGQDPVAFLQRFKDKVSHVHVKDVSQSLAAA
                     MRGELTGIAVSQTAIGDGVNAGNIRQCFDILAEIGYDGVVSIECEGQGGPMIEKSLKW
                     VRELANETNQRM"
     gene            2905..>3654
                     /locus_tag="PLC06_10370"
     CDS             2905..>3654
                     /locus_tag="PLC06_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRO90892.1"
                     /translation="MAIKLGINMEYVRHHDKPFEYGVRKAAEIGYEYVEPMVHWGREL
                     LSEARYFHSVSMYDDPLWAREVAESAGIKFSGLSTHTPLCKPEISVEYLKQAIRWAAD
                     AGAPVVNTDEGPKPLWTTREQDYTLMTYTLTEAARVAERHGIKIGLEPHQQYSKTPDG
                     LDSIYHLVDSPAIGINYDTGNSYLAGGSDPYEWLERVLDRLVHVHAKDISMEQGAAER
                     GKVTGTPVGCACGDGVVDWERVIALIKPVARD"
     assembly_gap    3655..3754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4054..5652
                     /locus_tag="PLC06_10375"
     CDS             4054..5652
                     /locus_tag="PLC06_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRO90893.1"
                     /translation="MARDHLIGVDLGTSSTKAALYRTDGTLVAEASADVPLLYPAPGV
                     VEQENDDFYRTAAETVGRCLRESDTDPRRVAAIAFSSQMAGIGSIDENYEPATRFDSW
                     LDMRCRPYIAWLNDNHADLITRLTGCAPTCDHGPKILWWMHERPEDYRRVAKFVTPSA
                     YVAGRMAGLPADRAFMDYTFIHFSGLADNRAGQWSAAICDTLGVDMAVLPEIVEPWRV
                     IGEVTEAAARDFGLAAGTPIAAGCGDTAANALGAGIVRPGQVFDVAGTASVLAGCTDH
                     FVADTTHRALLTMRSVIPGLWNPLAYIAGGGQALRWFRDEFYKYEIRNTKYELRSAPD
                     EIAASGEDSETRISYLVSRTYDEMIAEAAAVPPGSEGLFFSPHLGGRICPATPEMRGA
                     WLGFSWGHTRAHFFRAMLESIAYEYAWYLRVLREYIPNLELSEARAVGGGARSDDWNR
                     IKADVLGVPYQRLARGEFGTWGAAMIAGKAAGVYDDLAAVAAAHATPAGEPIRPSAAN
                     HEVYRPLVDRHVALQRQLVEIFTM"
     gene            5727..>6339
                     /locus_tag="PLC06_10380"
     CDS             5727..>6339
                     /locus_tag="PLC06_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRO90894.1"
                     /translation="MSSSVRICMIGAGRVGKLHSGSLRRHVAGGEVVALVDPFRPVLD
                     ETGDLFEIERRFTSLEDALDAVEFDAVVITTPTPTHRDLAVLAAEHGKHVFLEKPMAL
                     TLAECDDIIAATSARGVILQMGFMRRFDPDFAAAWERIQGGEIGEPMTIKSLTHGPGL
                     PPAWATDLKTSNGNLAEVNSHDLDTTRWLAASTPLRMYVEVANF"
BASE COUNT         1190 a         2005 c         1987 g         1057 t
ORIGIN      
        1 gcggcgtgtc gattgaggcc ggcacggggc tggagatgca atccatcgcc gcggcggtgc
       61 tgggcggcac gatgttgctg ggcggccgcg gctccgtccc ggcggccatg accggcgcgc
      121 tggcgctgga ggtgttgttt accctgctga atctgatggg cctgcccaag ccgctgcgcg
      181 acgccgtgca gggcctcatc atcatcggcg cggtggccta tacggcctat agcacgagga
      241 agacgagata attacgaatt acgaattacg aattacgaat gaagagcgtc ttccgattcg
      301 taattcgtaa ttgatatgag gaggtgatgc ctgatacggg atacttcaat aagcgagcgc
      361 agacctgtgg tcacgctcaa tgtatgttta aaaaaacctt ttaacttcaa tatgtggagg
      421 agagatccat gaaaccgttg cgagttttga ttttgatggt ggcgatgatg ctgatgatcg
      481 ctctggtcgc ctgtggcggc ggcgcggcga cgacccaggc ccccgcggcc acggaaccgg
      541 ccgccgaggc ccccgcggcc gaagaacccg cggccgaaga accggccgct gaagagcccg
      601 ccgcttcgac cggtgccggc ctcggcatgg aagccgcggc cgtggccgag cagttcttct
      661 ctcaggccga cctggaccgc tcaatcgctt tgctcgacgc gaccccgatg ggtccggaag
      721 gtcagccgtg ggtgcaatac ctggaagagc acttcgtcga cacgtcccaa tatgccaagg
      781 aaggccccta caccttctgc ttctccaacg cgggcgtcaa caacccgtgg cgcgtcgtcg
      841 gctacaacac catgaccgcc gaagttgaga tgcatcccga gatcgcggaa tggatccatg
      901 tcgacgccga aggcagcgac gagaagcaga tctccgacat ccaggacctg gtcgccggcg
      961 gtaactgcga catcctgatc gtcagcccga acacgaccca ggctctgacc ccggccgtgg
     1021 aagaggcctg caagagcctg cccgtcgtcg tcttcgaccg cggcgtgcag accgactgcc
     1081 cggctacctt tgtgcagccc atcggcggct acgcgttcgg ctatgtcggc gcgaagttcc
     1141 tggccgacaa tctggaaccc ggcgcgaagg tcgtcgcctt gcgtatcttg cccggcgttg
     1201 acgtgctgga gacccgttgg gcggccgcca agcaggtctt cgagcaggaa ggcattgacg
     1261 ttgtggaagt cgctttcggc gacggcaaca acgacaaggt caagactatc atcaccgacg
     1321 cgattcagaa gtacggccag atcgacggcg tatggatgga cgcgggcgcg acggccgtgg
     1381 ccgccatcga ggccttcgaa gacgcgggcg cgccctatcc cgtcatcaac ggcgaggacc
     1441 agcaggactt cctggtgaaa tggcagcagg aagggctgac ggccatcgcc ccgaccttcc
     1501 cgaccttcca gtggcgctcc gcagtcatcg ccgccctcat ggttttgcag ggcgagcagg
     1561 tcaatgaccc gtggattctg ccgcagccga ccgtgaccca ggagaatctg gatcagtacg
     1621 tccagcccga catgccgccg ctgttctatg cgatgtgcgg ctgcgagggc atgcccggct
     1681 tcccggagcg ctggcagtaa gcgagatacg aaatacgaga tacgagatac gagtgaagag
     1741 cgcgacgagc gagcaatgat ctgttcaacg tccgatacga acttgtatct cttgtctcaa
     1801 tgaggtgata tgaaatacga gatgcgggtg aggaaaatac tgtgcgagct ttaaccggct
     1861 cacagcacat tctgaactcg tatctcgtat ttcgtatctc gtattttgga gtgggggatc
     1921 atgattgaga ttggctttca taccgatgct ttcaacagcg cctactggag cttcgaacga
     1981 tgcttgcagt gggcgcagga caacgatgtc cactacatcg aatgcggcac catcgacggc
     2041 gtgagctgga tccacggcct cggctatcag ccccacgtcg ccctctggga agacccgctg
     2101 gccctgcgcg agaagatggc gcgctacggc gtccgcttct cgcagctgga cgcggccttc
     2161 cccctgtcct tgcccacggc cacgtcgctg ggcgaaacct atatccgaaa ctccatccgc
     2221 tgggcctatc tggcgggttg cgaccatatc gacacgaccg ataacatgca ccggcccgaa
     2281 ggcctctccg acgaagaggc gctggagatg atgaaacgca tctacggcaa cgtcctggaa
     2341 gacgccgcgc gctacggcat gaccatcaac atcgagccgc acggctatta caccaccaag
     2401 ccggattaca tggcgcgcat gctggacttc ttcgacacgc cccatctgcg catgaacatg
     2461 gacaccggca acaccttcat cgccgggcag gacccggtgg cgttcctgca gcgcttcaag
     2521 gacaaggtga gccacgtcca tgtgaaggac gtcagccaat cgctggcggc ggccatgcgc
     2581 ggcgagctga ccggcatcgc cgtcagtcag acggccatcg gcgacggcgt caacgccggc
     2641 aacatccgcc aatgcttcga catcctggcc gagattggct acgacggcgt ggtcagcatc
     2701 gaatgcgaag ggcagggcgg cccgatgatc gagaagagcc tgaaatgggt cagggagttg
     2761 gcgaacgaaa caaatcaacg aatgtagata cgaaatacga gatacgaaat acgagtcaag
     2821 aggcctcacg gcagggacga tcactcatcc ggacgacgtt ctgaactcgt atctcgtatt
     2881 tcgtatctcg tatctggaga aaacatggcg attaaactgg gcatcaacat ggaatatgtc
     2941 cgccatcacg acaagccgtt tgagtacggc gtgcgcaagg cggccgaaat cggctatgag
     3001 tacgtcgagc cgatggtgca ttgggggcgc gagctgctga gcgaggcccg ctatttccac
     3061 tccgtctcga tgtacgacga ccccctctgg gcgcgggaag tggccgagag cgcggggatt
     3121 aagttctcag ggttatcaac ccacacgccc ctgtgcaagc ctgagatcag cgtcgagtac
     3181 ctgaagcagg ccatccgctg ggcggccgac gccggcgcgc cggtggtcaa caccgacgaa
     3241 ggcccgaaac cgctctggac gacccgcgag caggactaca cgctgatgac ctacaccctg
     3301 accgaggcgg cgcgcgtggc cgagcgccac ggcatcaaga tcggtctgga gccgcaccag
     3361 cagtacagca agacccccga cgggctggac agcatctacc atctggtcga ttccccggcc
     3421 atcggcatca actacgacac cggcaacagc tatttggccg gcggcagcga cccctatgag
     3481 tggctggagc gtgtgctcga ccggctcgtc catgtccatg ccaaggacat cagcatggag
     3541 cagggcgcgg ccgagcgcgg caaggtgacc ggcacgccgg tgggctgcgc ctgcggcgac
     3601 ggcgtggtcg attgggagcg cgtcatcgcc ctcatcaagc ccgtggcccg cgacnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcgt ggtcgattgg gagcgcgtca
     3781 tcgccctcat caagcccgtg gcccgcgaca aggacatcat cctctccgtc gaatgcggca
     3841 ccatcccgca ggcggagcgg agtttcgagt atctgtcgaa gttgctgtag cggagaaagg
     3901 gagaaagggg gaaggggaga aagggtgaaa gggagagagg gtgaaaggga gaaagggaga
     3961 aagggagtac cctttcaccc cttctccccc gctcccctgc tccctttctc ccctgccccc
     4021 cttctcccct gcccccctgc accggagata gtaatggcac gtgatcacct catcggcgtc
     4081 gacctcggca ccagcagcac caaggccgcg ctctaccgga cggacgggac gctcgtggct
     4141 gaggcttcgg ccgatgtgcc gctgctctat cccgcgccgg gcgtggtgga gcaggagaac
     4201 gacgacttct atcgcaccgc ggccgaaacg gtcggccgct gcctgcgcga gagcgatacc
     4261 gacccgcgcc gggtggcggc catcgccttc agcagccaga tggcgggcat cggctccatc
     4321 gacgagaact acgagccggc cacgcgcttc gactcgtggc tcgatatgcg ctgccgcccc
     4381 tacatcgcct ggctcaacga caaccacgcc gacctgatca cccgcctcac cggctgcgct
     4441 cccacctgcg accacggccc gaaaatcctg tggtggatgc acgagcgccc cgaggactac
     4501 cgccgcgtgg ccaagttcgt caccccctcg gcctatgtgg ccgggcgcat ggccgggctg
     4561 ccggccgacc gcgccttcat ggattacacc ttcatccact tctccgggct ggccgataac
     4621 cgggccgggc agtggagcgc cgctatctgc gacacgctgg gcgtcgatat ggccgtgctg
     4681 ccggagatcg tcgagccgtg gcgtgtcatc ggcgaggtga ccgaggcggc cgcgcgcgac
     4741 ttcggcctgg ccgcgggcac gcccatcgcc gccggctgcg gcgacacggc cgccaacgcc
     4801 ctcggcgcgg gcatcgtgcg gccggggcag gtgttcgatg tggccgggac ggcgtcggtg
     4861 ctggccggct gcaccgacca tttcgtggcc gacaccaccc accgcgccct gctgaccatg
     4921 cgctccgtca tccccggcct gtggaacccg ctggcctata tcgcgggggg cgggcaggcg
     4981 ctgcggtggt ttcgcgacga gttttataaa tacgagatac gaaatacgaa atacgagtta
     5041 agaagtgcgc cggacgagat tgctgcctca ggcgaggatt ctgaaactcg tatctcgtat
     5101 ctcgtatctc gtacctatga tgagatgatc gccgaggccg ccgccgtccc gcccggctcc
     5161 gagggactgt tcttcagccc ccatctgggc ggccgcatct gcccggccac gccggagatg
     5221 cgcggggcgt ggctgggctt ctcgtggggc cacacgcggg cgcacttctt ccgcgccatg
     5281 ctggagagca tcgcctatga gtacgcctgg tatctgcgcg tgctgcgcga gtacatcccc
     5341 aatctggagc tgagcgaggc ccgcgccgtg ggcggcgggg cgcgctccga cgactggaac
     5401 cggatcaagg ccgacgtgct gggcgtgccc tatcagcggc tggcgcgcgg cgagttcggc
     5461 acatggggcg cggcgatgat cgccgggaag gcggccgggg tctacgacga cctggccgcc
     5521 gtcgcggcgg cccacgccac gcccgccggg gaaccgattc gcccctcggc cgcgaaccac
     5581 gaagtctatc ggccgctggt ggatcggcat gtggccttgc agcggcaatt ggtggagata
     5641 tttactatgt agcgttgtag cgcaagctgt cagcttgcga gaacggcgaa gacgcaacct
     5701 gacaggttgc gctacaagga gacctaatga gttcatctgt tcgcatctgc atgatcggcg
     5761 ccgggcgcgt cggcaaactc cattccggca gcctgcgtcg gcatgtcgcc ggcggcgagg
     5821 tcgtcgccct cgtcgatccc ttccggcctg tcctcgacga gaccggcgac ctgttcgaga
     5881 tcgagcggcg cttcacgtcc ctcgaagacg ccctcgacgc ggtggagttt gacgccgtcg
     5941 tcatcaccac ccccacgccc acccaccgcg acctggccgt gctggccgcc gaacacggca
     6001 agcacgtctt cctggagaag ccgatggccc tgacgctggc cgagtgcgac gacatcatcg
     6061 ccgccacgtc cgcccgcggg gtgatcctgc aaatgggctt catgcgccgc ttcgaccccg
     6121 acttcgccgc cgcctgggag cgcattcagg ggggcgagat cggcgagccg atgaccatca
     6181 agtcgctcac ccacgggccg gggctgccgc cggcgtgggc caccgacctg aagacgagca
     6241 acggcaatct ggccgaggtg aacagccacg acctggacac gactcgctgg ctggccgctt
     6301 ccacgccgct gcgcatgtac gtcgaggtcg ccaacttca
//
LOCUS       DAOMQG010000181         6334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5237_length_6334_cov_9.766364, whole genome shotgun sequence.
ACCESSION   DAOMQG010000181 DAOMQG010000000
VERSION     DAOMQG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5237_length_6334_cov_9.766364"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLC06_10385"
     CDS             complement(<1..929)
                     /locus_tag="PLC06_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRO90895.1"
                     /translation="MSPIRIARSFIAADSVAELVATGYDCPAPVSGKLFSKLLRTQDN
                     DHYLITTGDGRQYAFRLYQQGDRYRRTESDYLYEMDWLNFLHGRGLPVSWPIQRRDGG
                     YLSRLEAPEGTRYYAMFSLAHGDPLSLKDPEQLYTMGEMMARIHVASNDFTSPHARKP
                     VDLAYLLDRPLERIGRSWTNEHIGDLDLVTATAEEARDELQELIADFPADGWGPIGGD
                     FHQSSVYFEEADQPTFFNFDLCGPGWRAYDIAAFLSNADLIHTTTERAEAFFAGYFSV
                     RPLSDAEHAAIHPFLTIRRVWLMGAFAREDGLVG"
     gene            complement(1008..2162)
                     /locus_tag="PLC06_10390"
     CDS             complement(1008..2162)
                     /locus_tag="PLC06_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO90896.1"
                     /translation="MFSYFTAEQEAFRQTVRRFVTTEINPYVEAWEAARLWPAHDVLR
                     KMGDLGLLGLNYPEEYGGGGVDYWYNAVLLEELGRADCAAVPMGIAVHTDMATPALAE
                     FGSDDLKRRFLVPAIAGEMVSAIGVSEPDAGSDVASIRTRAVADGDDYVINGAKMWIT
                     NGTQADFITLLARTSGERGFRGMSLIIVPTTTPGFSVSRKLEKLGNHASDTAILTFED
                     VRVPQANRIGDEGMGFTLQMKQFQKERLAGALMSTAGMEKAVRMTINYTRERQAFGRP
                     LIDNQWIQFRLAELLTEIEALRQLNYHCVRLLVAGEDLTKEVSMAKLKAGRLAREVAD
                     TCLQFHGGMGYVEEYPMARYFRDARLLSIGAGADEIMLGIIAKYENISSR"
     gene            complement(2251..>3183)
                     /locus_tag="PLC06_10395"
     CDS             complement(2251..>3183)
                     /locus_tag="PLC06_10395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HRO90897.1"
                     /translation="AETNSDPFLNLALAAEHTSKLALGTAIAVTFPRSPAILAYTAWD
                     LQRFSRGRFVLGLGPQVKAHNVLRFGVKWEKPVKKMRETIEAIRAFWDCWQNGTRLDY
                     VGEFYKLALMTPFFDPGPINYPPPPIYIAAVNEQMLRLAGSHCDGVHIHALHSLAYLR
                     DVALPVIESGLARGGKKRSGFSINTAVFVIPTNDPAEARMAEDHVRQQLSFYMSTPSY
                     RPVLAHHGWEAVSDQLGQMARAGEWEAMKRLITDEMLDTFAVIGRWGQLPEIIHARYG
                     DLLDRVSYYLPFEPGINDTGWRNTIDGFKRINRR"
     assembly_gap    3184..3283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3324..5027
                     /locus_tag="PLC06_10400"
     CDS             3324..5027
                     /locus_tag="PLC06_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRO90898.1"
                     /translation="MNVSRLRRFVPFLVLAALVVVLVAAISPASAATPFSNQFPIQIL
                     AVNDFHGNLEPGTLSFTLPDGTRVPVGGAEYLATHIKQLTAQNPRNTVVVSAGDTIGA
                     SPLLSALFHDEPTIEAFNDMGVNFNAVGNHEFDEGLNELLRMQRGGCHPVDGCQDGDG
                     FGGADFRYLAANVFYEGTNRTILPAFRVRNFQHGIKIAFIGMTLEGTPSIVTPSGVAG
                     LEFRDEADTVNNLVGQLRRRGIRNIVVLIHEGGFPASGGLYNECPGVSGPIVDIVERT
                     NQEVDLFVTGHTHQAYNCVIDGRVVTSASSFGRALSKIDVVVGRRTGLIQSITANNML
                     VTRDVAPDSAMTALIAKYNAIAAPLANRIIGQITADILRAANPAGESALGDVIADAQL
                     DATDDPGFGDAVIAFMNPGGIRADLTYAGSAAGEDDGNVTYGESFTVQPFGNSLVTKT
                     MTGEQIKMLLEQQFDNPGVGQNRILQISEGFSYSWSVSAPKGSKVSDVMLNGAPLDMG
                     ASYRVTMNSFLADGGDGFSAFRLGTNPLGGAVDTDALEDYFVANSPVAPGPMDRITQL
                     P"
     gene            complement(5153..>6334)
                     /locus_tag="PLC06_10405"
     CDS             complement(5153..>6334)
                     /locus_tag="PLC06_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRO90899.1"
                     /translation="SAGANLLYQDEVFILGGRTFANQARLSAAGIPQVALVFGSSTAG
                     GAYIPGMSDYVVMVRGRAAVYLGGPPLVKMATGEESDDETLGGAEMHARASGLSDYLA
                     EDDADALRIGRLILSHVGQPKPVIGLTRRVAPEPPAYDPDELLGIIPADPRVPYDVRE
                     IIIRLVDGSRFLEFKPDYGATLVCGHAHLDGWPVGILGNNGVLFSESANKAAQFIQLC
                     NQTRTPLIYLQNITGFMIGAQHERGGIIKHGSKMLNAVATSTVPQFTVIVGGSYGAGN
                     YAMCGRALDPRFLWSWPNSRVAVMGGEQAAGVLGIVQTEQARKRGREADANQIAMTQM
                     MTRQKFDAESSPWYATARLWDDGILDPRDTRTALSIGLSVAHHVDYVTEGPPRYGVFR
                     M"
BASE COUNT         1145 a         1844 c         1997 g         1248 t
ORIGIN      
        1 ccgaccagac catcctcgcg ggcgaacgcg cccatgagcc atacgcggcg gatggtgagg
       61 aaggggtgga tggcggcatg ctcggcgtca ctgagggggc gcacggagaa gtatccggca
      121 aagaacgcct cggcgcgctc ggtcgtggtg tggatcagat cggcgttgga gaggaaggcg
      181 gcgatgtcgt aggcgcgcca gccggggccg cagagatcga agttgaagaa ggtcggctga
      241 tccgcttctt cgaaatagac gctgctctga tgaaagtcgc cgccgattgg cccccagccg
      301 tcggccggga agtcggcgat caactcctgc agttcgtcgc gagcttcttc ggccgttgcc
      361 gttaccagat cgagatcgcc gatgtgttca tttgtccagg agcggccgat gcgctccaat
      421 ggccggtcga gcagataggc caggtcgacc ggcttgcgcg cgtggggcga ggtgaaatcg
      481 ttggacgcga catggatacg agccatcatc tcgcccattg tgtagagttg ttcagggtct
      541 ttcaacgaca gcggatcgcc atgggccagc gagaacatcg cgtaatagcg cgtgccttcc
      601 ggtgcttcca gtcgggaaag atagccaccg tctcgtcgtt gaatgggcca ggagacgggc
      661 agcccgcggc cgtgcaggaa gttgagccaa tccatctcgt acaggtaatc cgattccgtc
      721 cgccgatagc gatcgccttg ctggtaaagg cgaaaggcgt attgccggcc gtcgccggtc
      781 gtgatcaggt agtggtcatt gtcctgggtg cgcagcagct tgctgaacag tttgccgctg
      841 accggcgcgg ggcagtcata cccggtggcc accagctcgg ccacggaatc ggcggcgatg
      901 aaggagcgag caatgcgaat gggggacatg ggttttctcc attggtcagt gtttagtagt
      961 taacaggtca gtgagcaaac cagtgatgat ttgtagttcg tcattcgcta ccttgaggag
     1021 atgttctcat acttggcaat gatacccagc atgatctcat ccgcgccggc gccaattgat
     1081 agcaggcgcg catcgcggaa gtagcgagcc atcgggtatt cttccacgta gcccatgccg
     1141 ccgtggaatt gcaggcaggt gtcggccacc tcgcgcgcca ggcgcccggc cttcaacttg
     1201 gccattgata cctctttggt caggtcttcc ccggccacca gcaagcgcac gcagtgatag
     1261 ttcagttgtc gcagcgcctc gatctccgtt agcaactcag ccagccggaa ttgaatccac
     1321 tggttgtcga tgagcggccg cccgaacgcc tgtcgttcgc gggtgtagtt gatggtcatg
     1381 cgcacggctt tttccatccc ggccgtgctc atgagcgccc ccgccaggcg ttccttctgg
     1441 aactgcttca tctgcagggt gaagcccatg ccttcatcgc cgatgcgatt ggcctggggc
     1501 acgcgcacat cctcgaaggt gaggatggcg gtgtcgctgg cgtggttgcc cagcttctcc
     1561 agcttgcggc tgacgctgaa gccgggggtg gtcgtgggga cgatgatgag cgacatgcca
     1621 cggaagccgc gctcgccgct tgtgcgggcc agcagggtga tgaagtcggc ctgtgtgccg
     1681 ttggtgatcc acatcttcgc gccgttgatg acgtaatcat ccccgtcggc cacggcgcgg
     1741 gtgcgaatcg aggccacgtc ggagccggcg tccggctcgc tgacgccgat ggcgctgacc
     1801 atctctccgg cgatggccgg aaccagaaag cggcgtttca gatcgtcgct gccgaactcg
     1861 gccagcgcgg gggtggccat gtcggtgtgg acggcaatgc ccatgggtac ggcggcgcaa
     1921 tcggcccggc ccaattcttc cagcaggaca gcgttatacc agtagtccac cccaccgccg
     1981 ccatattctt cgggatagtt gaggcccagc aagccgagat cgcccatctt ccttaacacg
     2041 tcgtgggccg gccaaagccg cgccgcttcc cacgcctcga cataggggtt gatctccgtg
     2101 gtgacaaacc ggcgcaccgt ttgccggaag gcctcttgtt cggcagtgaa gtaggagaac
     2161 atgcgctgtt ccttatatca aataacttga cgaagagcag atatgaatgg gagccgggct
     2221 gaaaaatgcc gggttctcct gcctgttgat ttatctgcga ttgatccgct tgaagccgtc
     2281 gatcgtgttg cgccagccag tgtcgttgat gcccggttcg aagggcaggt aatagctgac
     2341 gcgatccagc agatcgccat agcgggcgtg gataatttcc ggcagctggc cccagcggcc
     2401 gatgacggca aatgtgtcca gcatttcatc ggtaattagc cgcttcatgg cttcccattc
     2461 gcccgcgcgc gccatctgcc ccaactggtc ggatacagct tcccagccgt ggtgggccag
     2521 gacgggcctg taggaagggg tgctcatgta gaacgacagc tgctggcgca cgtggtcttc
     2581 ggccatgcgc gcctcggccg gatcgttggt agggatgacg aacacggctg tgttgatgga
     2641 gaagccggag cgctttttgc cgccgcgggc aaggccggat tcgatgacgg gcaaggccac
     2701 atcgcgcagg taagccaacg agtgcagagc gtggatgtgt acgccgtcac aatgcgaacc
     2761 ggccagacgc aacatttgct cattgacggc agcaatatag atcggcggcg gcgggtaatt
     2821 gatcgggccg ggatcaaaga agggggtcat cagcgccagc ttgtagaact cacccacata
     2881 gtcaaggcgc gttccgtttt gccagcaatc ccagaaggcg cggatggcct cgatggtttc
     2941 gcgcatcttc ttgaccggct tttcccattt gacgccgaag cgcaggacgt tgtgggcctt
     3001 cacctgtggc cccagaccca gcacgaatcg gccgcgcgag aagcgctgca aatcccaggc
     3061 ggtgtaggcc aggatagccg ggctgcgcgg gaaggtgacg gcgatagccg tacccagcgc
     3121 caacttgctg gtatgctcgg cggccagcgc gaggttgagg aacggatcgc tattggtttc
     3181 ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttcccc aacctatttc
     3301 agtactgtgc agaggagaga tatatgaacg tgtcaaggtt gcgtagattt gtcccgtttc
     3361 tcgtgctcgc ggcgttggta gtggtgcttg ttgccgctat ttcgccggcg tcagccgcga
     3421 ccccgttttc aaaccagttc cccattcaga ttttggctgt caatgacttc cacggcaatc
     3481 tggagccggg tacattgagc tttaccctgc cggacggcac gcgcgtgccg gttggtggcg
     3541 cggagtatct ggcaacccac attaagcagc taactgcgca aaacccacgc aacacggtag
     3601 ttgtctcggc tggcgatacg attggggcca gccccttgct ttccgccctg ttccacgacg
     3661 aacctacgat tgaagcgttc aacgatatgg gtgtgaactt caatgccgta ggcaatcacg
     3721 agttcgatga gggtctgaat gaactcctgc gcatgcagcg tggcggttgt catccggtcg
     3781 atggttgcca ggatggtgac ggattcggcg gcgccgactt ccgctatctg gccgcgaacg
     3841 tgttctatga gggcacgaat cgcaccattc tcccggcctt ccgggttcgc aatttccagc
     3901 acgggatcaa gattgccttc atcggcatga cgctggaagg cacgccgtcg atcgttacgc
     3961 cgtcgggcgt tgccggtctg gagttccggg atgaggccga caccgtgaac aatctcgttg
     4021 gtcagctgcg tcggcggggt atccgcaata tcgtcgtcct gattcatgaa ggtggcttcc
     4081 ctgcgtcggg cggcctctat aatgagtgcc ccggcgtttc cggccccatc gtcgatatcg
     4141 tggaacgcac gaaccaggaa gttgacctgt tcgtcaccgg ccatacccat caggcataca
     4201 actgcgtgat cgacggccgc gttgtgacca gcgcctcgtc ctttggccgc gcgctgtcga
     4261 agatcgatgt ggtggttggc cggcgcacgg gcctgattca gtccatcacg gccaataaca
     4321 tgctcgtgac gcgcgatgtg gctcccgatt cggcgatgac cgccctgatc gccaagtaca
     4381 acgccatcgc cgcgccgttg gctaaccgga tcatcggcca gatcacggcc gacattctcc
     4441 gcgcggccaa tcccgccggc gaatccgccc tgggtgatgt cattgccgac gcgcagctgg
     4501 acgccactga cgatcccggc tttggcgatg ctgttatcgc cttcatgaac cccggcggta
     4561 tccgcgccga tctgacctac gcaggtagcg ccgccggcga ggatgacggc aacgtgacct
     4621 acggcgagtc gttcactgtg cagccgttcg gcaacagcct cgtcaccaag acgatgactg
     4681 gtgagcagat caagatgttg ctggagcagc agttcgataa cccgggcgtg ggccagaacc
     4741 gcatcctgca gatctcggaa gggttcagct attcctggag cgtgagcgcg ccgaagggca
     4801 gcaaggtttc cgacgtcatg ctcaatggcg caccgctgga tatgggcgcc agctatcgcg
     4861 tgaccatgaa cagcttcctg gctgatggtg gcgacggctt ctcggccttc cggttgggca
     4921 ccaacccgct gggtggcgct gtcgataccg acgcactgga agattacttc gttgccaact
     4981 ccccggttgc gcccggtccg atggatcgca tcacgcagct gccgtaagtc ttatccactt
     5041 tgatctagca ggttggcggg cggaaacccg ctaacctgtc aggtccgcac tgtcataaag
     5101 agcgctggag cctgtgcaag gttccggcgc tttttatttg gccgaggcct ggctacatcc
     5161 gaaacacgcc gtagcgtggc gggccttcgg tgacgtaatc aacatgatgt gctaccgaca
     5221 agccaatgct gagagccgtt cgcgtgtcgc gtgggtcgag gatgccatcg tcccacaggc
     5281 gagccgtggc ataccagggc gacgattctg catcgaactt ttggcgggtc atcatttggg
     5341 tcatggcgat ctgattggcg tccgcttcac ggccgcgctt gcgcgcctgt tcggtctgaa
     5401 cgatgccaag cacgccggcc gcctgctctc cgcccatgac ggccacgcgg ctgttgggcc
     5461 atgaccacag aaaacgtggg tcgagcgcgc ggccgcacat ggcgtagttc cccgccccat
     5521 aggaaccccc gacgatgacc gtgaactgcg gcacggttga agtggccacg gcgttgagca
     5581 tcttgctgcc gtgcttgatg attcctccgc gctcatgctg cgcgccgatc atgaagccgg
     5641 tgatgttctg caagtagatc agcggtgtgc gggtctggtt gcagagctgg atgaattggg
     5701 ccgccttgtt ggcgctttcg gagaagagca cgccattgtt gccgaggatg cccaccggcc
     5761 agccgtcgag atgggcatgg ccgcagacga gcgtggcgcc gtagtcgggt ttgaattcaa
     5821 ggaaacgcga gccgtcaacc agtcggatga tgatttcacg cacgtcatag gggacgcgcg
     5881 ggtcggcggg gatgatgccg agaagctcgt cgggatcata ggccggtggc tcgggagcca
     5941 cacgacgggt gaggccaatg accggctttg gctgccccac gtgcgacagg atcaggcggc
     6001 cgatacgcag cgcgtcggcg tcgtcttcgg ccagatagtc gctgaggccc gatgcgcggg
     6061 catgcatctc ggccccgccg agcgtctcgt cgtcgctctc ctcgccggta gccatcttca
     6121 ccagtggcgg gccacccaga tagacggccg cccgcccgcg caccatcacc acatagtcgc
     6181 tcatgccggg gatgtaagcc ccgccggccg tcgaggaacc gaagacgagc gccacctgcg
     6241 gaatgcccgc ggccgacagg cgagcctgat tggcgaacgt gcggccgccg aggatgaaca
     6301 cctcatcctg atagagcagg ttggctccgg ccga
//
LOCUS       DAOMQG010000182         6232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5352_length_6232_cov_12.461875, whole genome shotgun sequence.
ACCESSION   DAOMQG010000182 DAOMQG010000000
VERSION     DAOMQG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5352_length_6232_cov_12.461875"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1036)
                     /locus_tag="PLC06_10410"
     CDS             complement(299..1036)
                     /locus_tag="PLC06_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90900.1"
                     /translation="MKNKQSLITWLIIFAILVGSGIMTIILPSLFGGSSATPLPSETT
                     TVTVPLPIPIGGRSEITLPSWQWMLGLGLLVPALVIGAGLTLALIYILLSRLVAKTTS
                     SASYQTKSTALDQRQTQKISKMRETRPTSAAPESTWRRWSIVTTAITILMFVAFVALL
                     FASVAFPSGQIIRNGAIVNIASMIVLVTVGITLLALVLWLRSDRINAINRTETMAIPW
                     DFIAVVLSGLLVVGLGIGVIAILNAPK"
     gene            complement(1138..1773)
                     /locus_tag="PLC06_10415"
     CDS             complement(1138..1773)
                     /locus_tag="PLC06_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO90901.1"
                     /translation="MTRIEIEGDFDESALIRRAKVDQEAFGLLYERYSQRIYQYIYYR
                     TSSEPDAEDLAARTFMRAWQHIGNYDDRGVPFSAWLYRIAHNLVANWHRDRSRRKIIS
                     LDDLSRWQVNEDGPEASALFAEDRATLLKAVRRLPADRQELLLLKFVEHLSNAEIGDI
                     MGRSEGAIKSLYHRTLMTLRDDLQPDIQPEPKTSSFRKLFGRLGEKGQDRE"
     gene            2060..2374
                     /gene="rplU"
                     /locus_tag="PLC06_10420"
     CDS             2060..2374
                     /gene="rplU"
                     /locus_tag="PLC06_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRO90902.1"
                     /translation="MYAIVESGGRQYRAEEGHSFSVEKLPYEVGDKVELGNVLLIADG
                     DSVHVGQPTVGGATVKATVVEQYRGKKILVWKYRAKQRYRRRRGHRQDYTRLRIDEIV
                     AG"
     gene            2408..2680
                     /gene="rpmA"
                     /locus_tag="PLC06_10425"
     CDS             2408..2680
                     /gene="rpmA"
                     /locus_tag="PLC06_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRO90903.1"
                     /translation="MAHKKGGGSTRNGRDSNAKRLGIKRYGGEYVGPGMIIVRQKGTK
                     YRPGNNVGVGSDYTIYSLIDGQVTFESRFGRKVISVYPAQTESVGE"
     gene            2688..2876
                     /gene="rpmE"
                     /locus_tag="PLC06_10430"
                     /pseudo
     CDS             2688..2876
                     /gene="rpmE"
                     /locus_tag="PLC06_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011125833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            2868..3134
                     /locus_tag="PLC06_10435"
     CDS             2868..3134
                     /locus_tag="PLC06_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRO90904.1"
                     /translation="MRRLQKREDIQVQTETVKQSHKVENFAVEEMELGTRVNNALAQA
                     GLVTVGDVLNLLKEGDDAVLALQGVGQTALIKIKRYMRDEQLID"
     gene            3242..3856
                     /locus_tag="PLC06_10440"
     CDS             3242..3856
                     /locus_tag="PLC06_10440"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRO90905.1"
                     /translation="MQSYGTHPRGRIEIICGSMFSGKTEEMIRRLRRAVIARQQVQAF
                     KPAIDSRYHVEKVTSHDGLHFQAQPVSQSKDILIAVDVETDVVAIDEVQFFDSDIIMV
                     CETLADRGKRVICAGLDQDFRGLPFGPMPELLARAEDVSKLHAICVVCGEDASRTQRL
                     IEGSPAAFDDPVVLVGAAEVYEARCRKCHQIAPASALTNGQGGR"
     gene            3858..4418
                     /gene="hpt"
                     /locus_tag="PLC06_10445"
     CDS             3858..4418
                     /gene="hpt"
                     /locus_tag="PLC06_10445"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRO90906.1"
                     /translation="MRDIWADIETVLIDEPTLQNRIGEMAAQIEADYADKDDLLLICV
                     LKGAFVFLSDISRGLKRAHSVDFMGISSYGKGTTSMGAVQIIMDLKAPIENRHVLIVE
                     DIIDSGRTLDYMRRNLLARSPASLRICSLLNKPARREIDVHVDYIGFDIPDEFVVGYG
                     LDFDELYRNLPFIAVLKPEVFAHLFG"
     gene            4447..5952
                     /locus_tag="PLC06_10450"
     CDS             4447..5952
                     /locus_tag="PLC06_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRO90907.1"
                     /translation="MTNSDVLALSPLVDRLRPILAAESQPVYLVGGTVRDALLGRPIH
                     DIDLVVANGAIPLTFRVANILNLPAYVLDQERDVGRIVAPGKDLTLDIARFRGPTLED
                     DLRGRDFTINAMALPIESQAARDIIDRHNGLGDLAAGQIRRIHEDSLTDDPVRAIRAA
                     RFAAQLGFELTAETAAAARAAAALLPSRISPERLRDELSKLLAGGAPHIGVALLQDLG
                     ILEYVLPEVAALDGLAQSPPHHEDVFRHTLSVLRFGAAIDALIMDEGDTSTEAWSTAV
                     ESLLAPYRRPMQDHLAILLDGGIQTRLLLMWGGLFHDIGKAVTRTIAPDGRIRFLGHD
                     GKGATMAEERLSSLSFSNEVRQRGRHIVEGHMRPLLLANDRHVPSRRTIYRYFRALGP
                     AGLDVALLAIADHFATYEGHLDQESWEPLRVVLETLIDTYFNGYEQTIAPARLLNGQA
                     IMDELGIAPGNEIGRLLRLLEEAQASGDVITRDEALSFIHRHHRERRSPDS"
     gene            complement(6002..>6232)
                     /locus_tag="PLC06_10455"
     CDS             complement(6002..>6232)
                     /locus_tag="PLC06_10455"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HRO90908.1"
                     /translation="VGGPSASRLTKEFEEIELLIRRIQSYGCVVKSLEAGLLDFLCER
                     DGRDVFLCWRYGEPSIQFFHELHSGYSGRQPV"
BASE COUNT         1430 a         1779 c         1746 g         1277 t
ORIGIN      
        1 agcgcgaaga tatactcaaa caggaagcgc aatcctacag tcgataatgc caccaggatg
       61 atgatggtaa taaaatgttt tactcgtccc attcctcaat tccctacttt tagattgagc
      121 caaacctctt acagttgcat gtccctgggt gaatcatgat ctaccctaac cccggaaaca
      181 atcggggcga cgagagcaaa taaatgacac gcaaaaagcc ggataatcgg agaatccgga
      241 ttagacaata gaaggccacg gcatagacag gccatggccg ataagcgcga gcgaaatttt
      301 actttggtgc atttagaata gcaatcacgc cgatccccag accaaccacc agaaggccgg
      361 acaggacgac tgcaatgaag tcccaaggga tagccatcgt ctccgtgcgg ttaatcgcgt
      421 taatgcgatc cgagcgcagc cataacacca gcgccaaaag cgtgattcca acagtgacca
      481 gaacgatcat cgatgcaata ttgacaatag cgccattacg aatgatctgc ccgctgggga
      541 aagccacact ggcaaacaac aaggcgacaa aggcgacgaa catcaggata gtaatggccg
      601 tcgtgacaat cgaccaacgc cgccacgtgg attcaggagc agcactcgtt gggcgagtct
      661 cacgcatttt ggaaatcttc tgggtctgtc tctgatccag agcagttgac ttcgtttggt
      721 agctcgcgct ggaggtggtt tttgccacga ggcgagagag gaggatgtag atgagagcca
      781 acgtcaaccc cgcgccgatg accagggccg ggaccagcag gcccaggcca agcatccatt
      841 gccagctggg caacgtgatc tcgctgcggc cgccaatcgg aatgggcaag ggaacggtga
      901 cggtggtcgt ctccgagggc aacggtgtcg ccgaacttcc cccaaacagg gagggaagaa
      961 tgatggtcat gatgccgcta ccaacaagaa tggcaaagat tatgagccag gtaatcaatg
     1021 attgcttgtt tttcaagagt ctatctccac taaatagcgc taccgtcaga ccatttgcgg
     1081 cctgcagcga aatatactat cacaattatc ttgttgcaac aggattgcac gccatctcta
     1141 ctcgcgatcc tgccctttct cccccaaacg gccgaataac ttgcgaaaag acgaggtttt
     1201 cggttccggc tgaatatccg gctgcaagtc gtcgcgcaag gtcatcagcg ttcgatggta
     1261 tagagatttg atagcgccct cgctgcgacc cataatatca ccgatttccg cgttcgaaag
     1321 atgctccaca aactttagca gcaacagttc ctgccgatcg gccggtagac ggcgaacagc
     1381 tttcaacaag gtcgcgcgat cttccgcaaa gagagcactc gcttccggcc catcctcatt
     1441 cacctgccag cgactgaggt cgtccagcga gatgatcttc cggcggctgc gatcccgatg
     1501 ccagttcgcc acaagattgt gggcaatgcg atagagccag gccgaaaacg gcacgcccct
     1561 gtcgtcataa tttccaatat gttgccaggc tcgcatgaat gtcctggccg ccagatcttc
     1621 cgcatccggc tcgctgctgg tgcggtaata tatatattgg tatattcgct gcgagtagcg
     1681 ttcgtagagc aggccaaaag cctcctgatc cactttggcg cgccgaataa gcgctgattc
     1741 gtcgaagtca ccctcgatct cgattcgtgt catgattttc gcagcgatca ggctcgtcgt
     1801 cgtgacgaag tctgacagga tagcgggcag tataacaaat gccactatcc tcggcgaccc
     1861 gacaagagga ttgattcccc ctctagccag gcacggcgca tccggcgcga aattttgaca
     1921 acccgccgga agcgatctat aattcccgtt cgcgtgacaa aggttcacca accacgcctg
     1981 cgtcacgatt gataaacaac aagtcattac gacgaaagcc ggcatcaagt acggatgccg
     2041 gttctttttg gaggttatgg tgtacgcaat tgttgaaagt ggtggccggc aataccgcgc
     2101 cgaagaggga cattccttct ctgttgagaa gttgccctac gaagtcggag acaaggtgga
     2161 actgggcaat gtcctgctga tcgctgacgg agattccgtt catgtcggtc agccgaccgt
     2221 tggcggggcc accgtgaaag cgactgttgt agagcaatat cggggcaaga agattcttgt
     2281 ctggaagtat cgggcgaaac aacgctaccg ccggcgtcgc ggtcatcggc aggattatac
     2341 gcgcctgcgc attgacgaga tcgttgccgg gtagcccggc caggcatttt taagggagag
     2401 atataagatg gctcataaga aaggtggcgg ctctactcgt aacggccgcg acagcaatgc
     2461 caagcgcctc ggcatcaagc gctacggtgg tgaatacgtt ggccccggta tgattatcgt
     2521 ccgccagaaa ggcacgaagt atcgccccgg taacaacgtg ggtgtaggca gcgactacac
     2581 gatctattcc ctgatcgatg gtcaggtgac attcgagagt cgcttcggcc gcaaggtcat
     2641 cagcgtctat ccggctcaga ccgagagcgt tggggagtaa atttgagatg aaggaatcaa
     2701 tccatccgaa gtggtatccc aacgcaaagg tgatcgtcga gggcgaggtc gtcatgaccg
     2761 tcggctcgac caaaccggag atcaacgtcg aagtctggtc gggtacgcat ccgttctata
     2821 cgggcacgca gcgcctgatg gacaccgaag gtcaggtcga tcgcttcatg cgccgtttgc
     2881 agaagcgtga agacatccag gtccaaaccg agacggtcaa acaaagtcac aaggtcgaaa
     2941 acttcgccgt tgaagagatg gagctgggga cacgcgtgaa caacgccctg gcccaggccg
     3001 gcctggtgac ggttggcgat gttctgaacc tgctgaagga aggcgatgac gccgttctgg
     3061 ctctgcaggg tgtggggcaa acggccctca tcaagatcaa gcgctacatg cgcgacgaac
     3121 aactgattga ctaggcgcag gccgccatac ggttttgaat agaaaaccga tcagaggata
     3181 ataaggcaga tacgttcaac agcgtgtctg ccttttttaa ttccaggaga cgatcgcaac
     3241 tatgcaatcc tatgggacgc acccacgagg acgaatcgaa atcatctgcg gcagcatgtt
     3301 tagcggcaag acggaagaga tgatccgccg cctgcgtcgc gccgtcatcg ctcgccagca
     3361 ggttcaggct ttcaagccgg ccattgactc ccgctaccac gttgaaaaag taacctccca
     3421 cgatgggctg catttccagg cccaacctgt cagtcaatcc aaggatatac tgatcgccgt
     3481 cgacgttgag accgacgtcg tggctatcga cgaagtgcag tttttcgata gcgacatcat
     3541 catggtctgc gaaactctgg ccgaccgcgg caagcgagtt atttgcgcgg gccttgatca
     3601 ggatttccgc ggcctgccgt tcggcccaat gcctgagctg ctggctcgcg ccgaagatgt
     3661 cagcaagctc cacgcaatct gcgtggtctg tggtgaggac gccagccgca cacaacgcct
     3721 catcgaggga agcccggcag cgttcgacga tccggtcgtt ctggtgggcg cggccgaggt
     3781 ctacgaagcc cgctgccgaa aatgccatca aattgcgccg gcatcggcgc tgaccaatgg
     3841 acagggaggg cggtaacatg cgtgacattt gggccgatat cgaaacggtg ttgatcgacg
     3901 aacccaccct gcaaaaccgg atcggggaaa tggccgcaca aattgaggcg gattacgccg
     3961 acaaggacga tctgctgctg atttgtgtat tgaaaggggc gttcgtattt ctgtccgaca
     4021 tcagccgcgg tcttaagcgc gcccacagtg tcgatttcat gggcatctcc agctacggca
     4081 aggggacgac cagcatgggc gcggttcaga taatcatgga tctgaaagcg cctatcgaga
     4141 atcgccatgt cctcattgtg gaagatatta ttgacagcgg ccgcaccctg gattatatgc
     4201 gtcgcaatct cctggcccgc tcaccggcct ctttgcgtat ttgctctctg ttgaacaagc
     4261 ccgcccggcg cgagatcgac gtccatgtcg actacatcgg tttcgacatc ccggacgaat
     4321 tcgtcgtcgg ctacgggctg gactttgacg agttataccg gaacctgccc ttcattgcgg
     4381 tcctcaaacc ggaagtattt gcccatttat ttggctaacc ggcgcgctgc atcgacgata
     4441 tttcatgtga ccaactcaga cgtattggct ctttctcccc tcgttgaccg gttgcgcccg
     4501 attcttgcgg ccgaatccca gccggtctat ctcgtcgggg gcacggtgcg cgacgcgctg
     4561 ttggggcgtc caatccatga tatcgatctc gttgtggcca atggcgcaat cccgttaacc
     4621 tttcgcgtgg ccaatattct gaacctgccg gcctatgtcc tcgatcaaga gcgggacgtc
     4681 ggccgcattg tcgctcccgg caaagacctg acgctcgata tcgcccgttt tcgcgggcca
     4741 actcttgagg atgatctgcg cggccgcgac ttcacgatca acgcaatggc cctgcccatc
     4801 gagagccaag ccgccaggga catcatcgac cgccacaacg ggctaggcga tctggccgcc
     4861 ggccaaatcc ggcgcatcca tgaagactct ctcaccgatg atccggtgcg ggcgatacgc
     4921 gccgcgcgat ttgccgccca gcttggtttc gagttgacgg ccgaaaccgc ggcagcagct
     4981 cgtgccgccg ccgctctgct gccttctcgc atctcacccg aacggttacg cgacgaactt
     5041 tccaaactcc tggccggcgg tgcgccccat atcggcgtcg ctctgctaca ggatttggga
     5101 atactggaat atgtgttgcc cgaagttgcc gcactggacg gcctggcgca atccccccca
     5161 catcacgagg atgtctttcg gcacacgctg agcgtcctgc gatttggggc ggcgatcgac
     5221 gcgttgatca tggacgaggg agatacctcc acggaagcct ggtcaactgc tgtcgagtca
     5281 ttgctggctc cctaccgacg gccgatgcaa gatcatctgg cgatcctgct cgacggtggc
     5341 atccagacac ggttgctgtt gatgtggggc ggcctgttcc acgacatcgg caaagccgtc
     5401 acccggacga tcgcgcccga cggccgcatt cgcttcctgg gtcatgatgg aaaaggggca
     5461 acaatggccg aggagcgcct ctcgtcgctc agcttcagca acgaggtccg gcaacgcggt
     5521 cgccatatcg tcgagggcca catgcggcca ttgcttctgg ccaacgatcg gcacgtgcca
     5581 tcacgccgca ccatctatcg ctacttccgc gctctggggc cggccgggct ggatgtggct
     5641 ttgctcgcca tcgccgatca tttcgccact tacgagggcc atctcgatca ggagagttgg
     5701 gagccgcttc gcgtcgtgct cgaaacactc attgatacct atttcaacgg atatgagcag
     5761 acgattgcgc ctgcccggct tctgaacggg caggctatca tggacgagct tgggatagcg
     5821 ccggggaacg agatcggccg cctgttacgt ttgctggaag aagcccaggc atccggcgac
     5881 gtgatcacgc gggatgaagc gctatcgttc attcatcgtc atcacaggga acggcgaagc
     5941 ccggattcat gaaaaagcgg ccatgtggcc gctcattgat ggttaaattc aggcgcgtac
     6001 gctacaccgg ctggcgaccc gaataccctg aatgcaattc atggaaaaat tgaatcgagg
     6061 gttccccata gcgccagcac aggaaaacat cccgcccatc ccgttcgcac agaaagtcca
     6121 gtagaccggc ctcaaggctc ttgaccacgc agccatagga ctggatgcgc ctgatgagta
     6181 gctcgatctc ctcaaactct ttcgtcagcc gagaggcgct cggcccgccc ac
//
LOCUS       DAOMQG010000183         6211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5379_length_6211_cov_9.736355, whole genome shotgun sequence.
ACCESSION   DAOMQG010000183 DAOMQG010000000
VERSION     DAOMQG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_6211_cov_9.736355"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="trpS"
                     /locus_tag="PLC06_10460"
     CDS             complement(<1..724)
                     /gene="trpS"
                     /locus_tag="PLC06_10460"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRO90909.1"
                     /translation="MNKKRVFSGIQPTGNIHLGNYLGAVRNWVREQSEKINYFCIVDL
                     HSLTVPQDPERLRFETRSMGAILLACGIDPQASTLFIQSHVAAHAEGCWLLNCITPLG
                     WLQRMTQFKDKSAKQESVLTGLLDYPVLMAGDIIFYDADEVPVGEDQKQHVELARDIA
                     QRFNALYNLDFFVVPQPVIPKVGARVMGLDDPTVKMSKSLADRRGHAIRLLDEPKEVM
                     YAFKRAVTDSGREIRFSDDPEKA"
     gene            complement(806..2368)
                     /gene="trpE"
                     /locus_tag="PLC06_10465"
     CDS             complement(806..2368)
                     /gene="trpE"
                     /locus_tag="PLC06_10465"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRO90910.1"
                     /translation="MIQDTTRVTYAPALDEFRALARGPANLIPVTREFAADLETPVSV
                     YLKLMDEQGASFLLESVEGGEQVGRYSFVGVNPRATIALRGRVVERNEEGKGSGGEGE
                     KSATFRELNEGEDILHVLKAELARFHAADLPGLPRFAGGAVGYLGYDVVRSFEKLPDT
                     AAPTLDVPDAMFLLADTLVVFDHARHRLILLANASIEGDNPDVEAAYFDALRRIDRLA
                     EKLLRPLPAVPTRRWGATHGNGQEIGVNMTRERYEEIVREAKEYIAAGDIFQVVLSQR
                     FSRRTSAHPFAVYRALRMLNPSPYMFYFNFAPLDLQVIGASPEMHVRLEDGTATVRPI
                     AGTRRRGANPAEDAALATELLADPKERAEHVMLVDLGRNDIGRVSQYGSVHVRDLMTV
                     ERYSHVMHIVSQVEGRIRPELDAYDLMRATFPAGTVSGAPKVRAMEIIEALEGQRRGL
                     YAGAAGYFSYDGSMDTCIAIRTMVMQGDSVSVQSGAGIVADSDPAAEYQECVNKAGAL
                     LVAVARAEAGTL"
     gene            complement(2641..4002)
                     /locus_tag="PLC06_10470"
     CDS             complement(2641..4002)
                     /locus_tag="PLC06_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRO90911.1"
                     /translation="MTAQTKYLLDESKLPKAWYNINADMPVAPAPVLHPGTLEPVTPD
                     FLAVLFPMSLILQEISAERYIEIPEPVREVYKLWRPTPFIRARRLEKALDTPAHIYFK
                     YEGASPVGSHKPNTAVPQAFYNKQEGTKALTTETGAGQWGSALAMACNFFGIDLEVYM
                     VKESFYQKPYRRIMIESYGAQVFASPSDRTHYGRALLEGNADAPGSLGIAISEAVEAA
                     AASGGTKKYSLGSVLNHVLMHQSVIGLETLQQFEMADEYPDVIIGCTGGGSNFAGFAF
                     PFIHKNLTEGKTTRVIATEPAASPSLTKGVYTFDYGDTAQMAPVVKMHTLGHTFIPPG
                     IHAGGLRYHGMAPQISALVDSGHIEARAVKQLDTFKAALTFLRAEGIIPAPESSHAIK
                     VAIDEALAAKESGEKRVVAFNLSGHGHFDMAAYEAYLHGQLEDYEYPAEAVAEALQHL
                     PAV"
     gene            4644..5774
                     /gene="nirK"
                     /locus_tag="PLC06_10475"
     CDS             4644..5774
                     /gene="nirK"
                     /locus_tag="PLC06_10475"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HRO90912.1"
                     /translation="MTRERLILVIVIVLALVGLGMGADAFFASRTTQNQLNAAVATAE
                     ASTAATNALISNIKVSPTLEPAHQTDVPNVAYDPANVPPPINRTEPTTVKFTLTTYEV
                     TAELADGTTYDFWTFDNTVPGPMIRAMVGDTIEMTLVNPADSMSGHNIDLHAVNGPGG
                     GAEVTNVLPGETKTFSFKALNPGIYVYHCAYAPPQHHIAQGMYGAILIEPVGGLPPVD
                     REFYVMQGDWYTLGSFGSQGHQVFSSEKALAEAPEYFTMNGHVNALTKLYPLQAKVGE
                     TVRVFFGVGGPNVGSNFHVIGEVFDKVFTGSPETFIANEEAWYVPPGSVAAFEMHLDV
                     PGNYVLVDHALYRTQKGAAGILHVTGDWNTDIFSPEVEGFSN"
     gene            complement(5919..6116)
                     /locus_tag="PLC06_10480"
     CDS             complement(5919..6116)
                     /locus_tag="PLC06_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90913.1"
                     /translation="MAYKFTNSKGKDYFLHARIVELKGGRKQQLYFFAKEPKDGALDT
                     VPAGYQVEESKNGLPVLKKAN"
BASE COUNT         1147 a         1870 c         1874 g         1320 t
ORIGIN      
        1 ccgccttttc gggatcatcg gagaagcgaa tctcgcggcc gctgtcggtg acggctcgct
       61 tgaaggcgta catcacctct ttcggctcgt cgagcaggcg gatggcgtgg ccgcgccggt
      121 cggccaggct ctttgacatc ttcacggtcg ggtcgtcgag acccatcacg cgcgcgccca
      181 ccttggggat gaccggctgg gggacgacga agaaatcgag gttatagagc gcgttgaatc
      241 gctgggcgat gtcgcgggcc agctccacgt gttgtttctg gtcttcgccc accggcactt
      301 cgtcggcgtc atagaagatg atgtctccgg ccatcagcac cgggtaatcg agcagaccgg
      361 tcagcacgct ttcctgtttg gccgatttgt ccttgaactg ggtcatccgt tgcagccagc
      421 cgagcggcgt gatgcagttg agcagccagc agccctcggc gtgggcggcc acgtggctct
      481 ggatgaatag cgtgctggct tgcggatcga tgccgcaggc cagcaagata gcgcccattg
      541 accgcgtttc gaagcgcagc ctctccgggt cctgcggcac ggtcaggcta tggagatcga
      601 caatgcagaa gtagttgatc ttctccgact gctcgcgcac ccaattgcgc accgcgccca
      661 gataattgcc caggtgaata ttccccgtgg gctgaatacc gctgaatact cgttttttgt
      721 tcatatctct tttggtagat acgagatacg aaatacgaga tacgaaagga ggaacgctct
      781 tcattcgtaa ttcgtaattc gtaatttaca gtgttcctgc ttccgcccgc gccaccgcga
      841 ccagtagtgc gccggccttg ttgacgcatt cctgatattc ggccgcgggg tcgctgtcgg
      901 caacgatgcc cgcgccggac tggacagata cgctatcgcc ctgcatcacc atcgtgcgga
      961 tggcgatgca ggtgtccatg ctgccgtcat agctaaaata gcccgccgcg ccggcataga
     1021 ggccgcgccg ttgtccttcc agcgcttcga tgatctccat cgcccgcacc ttgggcgcgc
     1081 cgctgaccgt accggccgga aaggtggccc gcatcaggtc gtaggcgtcc agctccggcc
     1141 ggatgcggcc ctctacctgg ctgacaatgt gcatgacatg actatagcgc tcgaccgtca
     1201 tcaggtcacg cacgtggacg gagccgtatt ggctgacgcg gccgatgtcg ttgcggccca
     1261 gatcgaccag catcacgtgt tcggcccgct ccttggggtc ggccagcagc tcggtcgcca
     1321 gcgctgcgtc ttcggccgga ttggccccgc ggcgtcgcgt gccggcgatc ggccgcacgg
     1381 tggccgtgcc gtcttccaac cgtacgtgca tctccggcga cgcgccgatg acttgcaggt
     1441 cgagcggcgc gaagttgaaa tagaacatgt agggcgacgg gttcagcatc cgcagcgcgc
     1501 gatagacagc aaacgggtgg gcgctggtgc ggcggctgaa gcgttggctc agcaccacct
     1561 ggaagatatc cccggcggcg atgtactctt tcgcctcgcg gacgatctct tcatagcgct
     1621 cgcgggtcat gttgaccccg atctcctggc cgttgccgtg ggttgcgccc cagcggcggg
     1681 tggggacggc gggcagcggc cgcagcagct tctcggccag ccgatcgatg cgacgcagcg
     1741 cgtcgaagta ggccgcttcc acatcggggt tgtcgccctc gatgctcgcg ttggccagca
     1801 ggatgagccg gtggcgggcg tggtcgaaga cgacgagcgt gtcggccagc aggaacatgg
     1861 cgtcgggcac gtccagcgtg ggcgcggccg tgtcgggcaa cttctcgaag gagcgcacca
     1921 cgtcataacc caggtagccg accgcgccgc cggcgaagcg cggcagcccc ggcaggtcgg
     1981 cggcgtggaa acgcgccagc tcggccttca ggacatggag gatgtcttcg ccctcattca
     2041 gttcgcgaaa ggtcgcggat ttctcccctt ctcctccgct ccctttccct tcttcattcc
     2101 tctccaccac acgaccgcgc agagcgatcg tcgcccgcgg attgacgccg acgaaggagt
     2161 agcggccgac ctgctcaccg ccttcgaccg actccagcag gaacgatgcg ccttgttcgt
     2221 ccatcagctt cagatagacc gacaccggcg tctccaggtc ggcggcgaac tcgcgcgtca
     2281 ccgggatgag attggccggg ccgcgagcca gcgcgcggaa ctcgtccagt gcgggggcgt
     2341 aggttactcg ggttgtgtcc tggatcatag tgtttcagta ccctgtatat cagtaggtca
     2401 gtaggtcagt aggtcagtag gtcagtgggt cagtagatca gtgggtcagt agatcagtgg
     2461 gtcagtgggt cggtgggtca atgggtcaat gggtcggtgg gtcaatgggt cagtgggtca
     2521 gtgggtcagt gggttatact gaatactgac ctactgaata ctgatctact gaatactgac
     2581 ctactgaata ctgacctact gatctactga cccactgacc taccggatac tgacttccta
     2641 tcatacggcc ggcaaatgct gcaacgcctc tgctactgcc tcggccggat actcgtaatc
     2701 ctccagctgc ccgtggagat aagcctcata ggcggccatg tcgaagtggc cgtggccgga
     2761 caggttgaac gccaccaccc gcttctcgcc actctctttg gccgccagcg cctcgtcgat
     2821 ggccaccttg atggcatggg acgattccgg cgcggggatg atgccctcgg cccgcaggaa
     2881 ggtcagcgcc gccttgaagg tgtccagttg tttgacggct cgcgcctcga tgtggccgct
     2941 gtcgaccagc gcgctgatct gcggggccat gccgtggtag cgcaggccac cggcatggat
     3001 gcccggcggg atgaaagtgt gacccagcgt gtgcatcttg accaccgggg ccatctgggc
     3061 tgtgtcgcca tagtcgaagg tgtagacgcc cttggtcagg ctgggcgacg cggccggctc
     3121 ggtggcgatg acgcgcgtgg tcttgccttc ggtcaggttc ttgtggatga acgggaaggc
     3181 gaaaccggcg aagttggagc cgccgccggt gcagccgatg atgacgtcgg ggtattcgtc
     3241 ggccatctcg aactgctgca gcgtttccag cccgatgact gactgatgca tcagcacgtg
     3301 gttgagcacc gagccaaggc tgtatttctt cgtcccgccg ctggcggcgg ccgcctccac
     3361 cgcttcactg atggcgatgc ccagcgagcc gggcgcgtcg gcgttgcctt ccagcagcgc
     3421 gcggccgtag tgagtgcggt cgctggggct ggcgaaaacc tgcgccccgt agctctcgat
     3481 catgatccgc cggtagggct tttggtagaa ggattccttg accatataga cctcaaggtc
     3541 aatgccgaag aagttgcagg ccatggccag cgccgacccc cactgccccg cgccggtctc
     3601 ggtggtcagg gcttttgtgc cttcctgctt gttatagaac gcctggggca cggccgtgtt
     3661 gggcttgtgg ctgcccaccg ggctggcccc ttcgtacttg aagtagatat gggccggtgt
     3721 gtcgagcgcc ttctccagtc gccgggcgcg gatgaagggg gtcggccgcc acagcttgta
     3781 gacctcgcgc accggctcag ggatctcaat atagcgctcg gcgctgatct cctgcaggat
     3841 gaggctcatg ggaaacagca cggccaggaa gtcaggtgtc accggctcca gcgtgcccgg
     3901 atggagcacc ggcgcgggtg ccaccggcat gtcggcgttg atgttgtacc acgccttcgg
     3961 aagcttgctc tcgtccagta aatacttcgt ttgtgcggtc atgatggttc tcctctatat
     4021 aaattaaatt cgtattcgta ttcgtaatcg caatcgtatt cgtaatcgta atcgagaatc
     4081 gacaacgatt gcgccagcga ttgcgactgt caccaaaaca aaaacgctct cgtccctact
     4141 caggacgaga gcgttcatat ctcccgtggt gccaccctgc ttaggccgcg catacaagca
     4201 cgacctcact cttgtcggcc cggtcttggt gatcagtgta cagtgatcag tgaacagttt
     4261 gaactgacaa actgattgac tgacctactg ataccttcaa caggccccgg ccctataacg
     4321 ggagccaccc cggccggcgc tactgataag caagctaatg acttgcgtga taggttcgcc
     4381 ccggcgactc atcggcccat tcacccaccg cgctgacgcc ggtctttcag cttccgaccg
     4441 gctctctgcg acccgcttgg cgggatacta ttccgattcg acgtcgtttg ttatttgatt
     4501 atggccagca gtctagcatg gcaggtcgaa cgtgtcaaga aggttttcct catgttgtat
     4561 taatcacttg atcgctgtta taatttcgcc ctgattgttg ctcgggatta gtttaaacaa
     4621 tacaaacatc cgaggaggat tatatgaccc gcgaaagact gatccttgtg atcgtcattg
     4681 ttctagccct ggttggtttg ggtatgggcg cggatgcatt ttttgcttcc aggaccaccc
     4741 aaaaccagtt aaacgctgcc gtcgccacgg ctgaagcatc gactgcggct acgaatgcac
     4801 tcatcagcaa tatcaaggtg tcgcccacac tggagccggc acaccaaacc gatgtcccca
     4861 acgtcgccta cgacccggcc aacgtgccgc cgcccattaa ccggacggag ccgacgaccg
     4921 tgaagttcac cttgacgacc tacgaagtga cggccgagtt ggccgacggc acgacctacg
     4981 atttctggac gttcgacaac acggtacccg gcccgatgat ccgggccatg gttggcgaca
     5041 cgatcgagat gaccctggtc aacccggcgg acagcatgag cggtcacaat attgacctgc
     5101 acgcggtaaa tggccccggc ggcggagcag aagtcaccaa tgtgctgccc ggtgaaacga
     5161 agaccttctc cttcaaagcg ctcaatcccg gcatctacgt ttaccattgt gcctacgcgc
     5221 cgcctcagca tcacattgct cagggcatgt atggcgcaat cctcatcgag ccggtcggtg
     5281 gtctgcctcc ggttgatcgt gagttctacg tcatgcaggg cgactggtat accctcggca
     5341 gcttcggcag ccaaggccat caggtcttca gctccgagaa agcgctggcc gaggcgccgg
     5401 aatacttcac catgaacggc cacgtcaacg ccctgaccaa gctttatccg ttgcaggcga
     5461 aggtcggcga gacggtgcgc gtcttcttcg gcgtcggtgg cccgaatgtc ggttccaact
     5521 tccacgtcat cggcgaagtg ttcgacaagg tcttcacagg ctcgcccgaa acattcatcg
     5581 ccaatgagga agcctggtat gtgccgcccg gttctgtggc tgctttcgag atgcatctgg
     5641 atgtgccggg caattatgtg ctggtcgacc acgcgctcta tcgtacgcag aagggcgcgg
     5701 ccggcatact ccatgtcacc ggcgattgga ataccgatat cttctcgccc gaagtagagg
     5761 gtttcagcaa ctgatcccga agcaacaggt cgacggaggc tgacgataag agaaacaggg
     5821 atctgtgcca ggattcgtca gagcgacaga aattgaaaca aaaaggcgga gccgaaatcg
     5881 gccccgcctt ttttcatgat ctgtgacttc gatccgggtt agttggcctt cttcaacacg
     5941 ggcaggccgt tcttcgactc ctcgacctga tatccggcgg gcactgtgtc gagcgccccg
     6001 tctttcggct ccttggcgaa gaagtagagc tgctgcttgc ggccgccctt caattcgacg
     6061 atccgcgcgt ggagaaagta atccttgcct ttgctgtttg tgaatttgta tgccatttcg
     6121 gcacctccta tgctgtctga cgactttttc ctttccaaca tacttttcaa aacaaaggcg
     6181 ctctcccttc gcagggtcgg cgccgtgtcc c
//
LOCUS       DAOMQG010000184         6201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5390_length_6201_cov_9.677351, whole genome shotgun sequence.
ACCESSION   DAOMQG010000184 DAOMQG010000000
VERSION     DAOMQG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5390_length_6201_cov_9.677351"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..318
                     /locus_tag="PLC06_10485"
     CDS             37..318
                     /locus_tag="PLC06_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRO90914.1"
                     /translation="MRYEWDESKRLSNIRKHGFDFRDAHEIFDGPMLIGLDIRSDYGE
                     DRWIGMGFLREIVVVIIYTESNKSDVRRVISLRKALNYERKRFEAHLSN"
     gene            296..547
                     /locus_tag="PLC06_10490"
     CDS             296..547
                     /locus_tag="PLC06_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HRO90915.1"
                     /translation="MKHTSQTDWERIRAMADEEIDTGDIPPLDDDFFAHAELRLPKPK
                     QSVTIRLDADVVEWYKAQGKGYQTRMNAILRRYMEVHMM"
     gene            complement(600..1241)
                     /locus_tag="PLC06_10495"
     CDS             complement(600..1241)
                     /locus_tag="PLC06_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO90916.1"
                     /translation="MEPIRILICDDHDAFRQGLRAMLATDASLNVVGEAADGAAAVTQ
                     AAELQPDVILMDLQMPRLNGLEATRRIVAAGPHIRILVLTMSDDTATVFHALQAGARG
                     YILKGARKADVVRSIQNVFNDEVVFGAAVAQHVIHHFTRVQADVQAKAFPQLSERESE
                     ILNLMAQELPNEAIAERLSISLKTVRNHVANICTKLQVSDRTAAVLRAKGSGG"
     gene            complement(1250..2518)
                     /locus_tag="PLC06_10500"
     CDS             complement(1250..2518)
                     /locus_tag="PLC06_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRO90917.1"
                     /translation="MTGESPLPAGDGRRGIPWRGFLSTLLLVLLLIGWWTLVYTFLVA
                     GWWLINGYTFQEGPGNPYAWNGLVDWILMILALLSVWPAQRWLRPRVALFVEDNNDDP
                     YTTISRISARLDTELTAEALPLAIAHSLTEILNLPWVELETSEGATAESGPRPDGPLV
                     TLPLQYNGRALGELRLVPRVTAGFPLPIDERLLGDLTRQISLALYNNQLAAELQESRR
                     HLVTAGEEGRRVLRRDLHDGLGPALATMTMQAETARELLRDDPAAADRALTGLIDQAQ
                     ATVSEVRRIVHGLRPPALDELGLFGALEVLADGFAAPGLRVELRLPKEGRALPAAVEV
                     AVYRIAQEALTNVSRHARAQTATLALWFENDGLRLEISDDGVGLPSEPELGIGLTGMR
                     ERAEEIGGTLTVLPNRPRGTCITARFPIET"
     gene            complement(2750..3307)
                     /locus_tag="PLC06_10505"
     CDS             complement(2750..3307)
                     /locus_tag="PLC06_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90918.1"
                     /translation="MKSETNTRPFALFGMAGAAFFFLNLIVEYRYDLFPPASGPLFVA
                     NQLGFFVGMIGLLIMLIGLWRARAAGEGRFGRLALGLFIAAWATLILGGFISLFTDAG
                     LILQPIGGLGGLLGALLTGIAVVLARRWTGWQRFAPLAQGLFIPAMMIVRGTFDPTFI
                     IEAVWMGLWFLTSLALYTARPRPGE"
     gene            complement(3310..3693)
                     /locus_tag="PLC06_10510"
     CDS             complement(3310..3693)
                     /locus_tag="PLC06_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90919.1"
                     /translation="MNHSPLYKTAVILFALFSLVNVVATMPPLLGGPEDQGFAAGIPQ
                     GVIILAAMSGVAGLFAAYGAWRGQKWGVWLTLFLCAVGILSALPGVLFAPNNAARASA
                     IIGIVVSIFVIVALLRRPKAAASGR"
     gene            complement(3793..4170)
                     /locus_tag="PLC06_10515"
     CDS             complement(3793..4170)
                     /locus_tag="PLC06_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90920.1"
                     /translation="MNHSPLYKTAIILFALLCVLTVVTFIPALLGGPDNQSAVAGIPQ
                     VIIIFATLVSVVGLVAAYGAWQGQKWGKWLVIILSAVNGLSALPGVTAAPSDFARISA
                     IVTVLVSVFVIVVLLLRPKIIAS"
     gene            complement(4271..5215)
                     /locus_tag="PLC06_10520"
     CDS             complement(4271..5215)
                     /locus_tag="PLC06_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90921.1"
                     /translation="MKHADSINRRTWSILTLVVIAVLALAAACSGSGAQQAAAPAPTA
                     VPPTAVPPTEAPAKAEIPVVTVEAKDGAFNVPEVVPGGIVRFDITNAGSDPASPTLAR
                     VKEGHTSQEVLDANARAAADPGAALGALEVANIVHVIGDIAPGATKTFYADLRTGEFG
                     IGDELRPDTLMVFFKADEIVGTTEPETAVTVDMVDFAYTMPDTVPAKGLWEFINTGDQ
                     WHMAVMTTYNPDVTPEQLLGMFSEEGGPPPADAPIQVLETGMPPMSPGERVWVEMDLQ
                     PGQYQLVCPLPDLVAMAAGEEPMPHLLHGMMHMFTVEN"
     gene            5489..>6201
                     /locus_tag="PLC06_10525"
     CDS             5489..>6201
                     /locus_tag="PLC06_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRO90922.1"
                     /translation="MITSLANPQVKYVRRLQSDRRFRANERAFVVEGTRWMRDLVAAR
                     AVPRALFATAAWLESNEGRGLSATLGAAVAVSDEVMAAMSDTETAPGVLAVLPIVPRP
                     WPDAPALVVILDAVGNPGNLGTILRAAGAAGADGVLLGPGCVDAYNPKVVRGSMGALL
                     RLPVRAASWADIGALTAGLDVWLAAADGATAYTAVDWRRPSAILIGGEAHGAGAAAGQ
                     LATGRVTIPMHDATESLNAA"
BASE COUNT         1201 a         1912 c         1971 g         1117 t
ORIGIN      
        1 ttgatgtatt gacacaaagt atgtacaata ataattatgc ggtacgaatg ggatgagagc
       61 aagcgcctgt caaatatccg caagcatggg tttgactttc gcgacgctca tgaaatcttc
      121 gatgggccaa tgttgatcgg tctggacatc cgctctgatt atggcgaaga tcgatggatc
      181 ggcatgggct tcctccgaga gatagttgta gtcatcatat atacagaatc taacaaaagc
      241 gatgtccgac gggttatctc cttacgaaag gcattgaatt atgaaagaaa acgatttgaa
      301 gcacacctct caaactgact gggaaagaat acgggccatg gccgacgagg agatcgatac
      361 cggcgacatt cccccgctgg atgatgattt cttcgcccat gccgaattac gattgcctaa
      421 gccgaaacag tccgtcacca ttcgtctgga cgcggatgtg gtcgagtggt acaaggccca
      481 gggcaaaggc taccagacgc gcatgaatgc cattttgcgg cgctacatgg aagttcacat
      541 gatgtagatt gttaatagaa accggggttc ccggaagaaa cctggtttcc gaccccgtct
      601 caccccccac tccccttcgc ccgcaacacg gccgccgtcc ggtcgctcac ctgcaacttg
      661 gtgcagatat tggccacgtg attgcgcacc gtcttcaggc tgatgcttag ccgctcggcg
      721 atggcctcat tgggcagctc ctgggccatc aggttgagga tttccgactc gcgctcgctg
      781 agttgcggga acgccttggc ctgtacgtcg gcctgcacgc gggtgaagtg atggatgacg
      841 tgctgggcca cggccgcgcc gaacacgacc tcatcgttga aaacgttctg gatggagcgc
      901 acgacgtcgg ccttgcgcgc ccctttcagg atataacccc gcgccccggc ctgcagggcg
      961 tggaagacgg tggccgtgtc gtcggacatg gtcagcacga ggatgcgtat atggggaccg
     1021 gcggccacga tgcgccgcgt ggcctccagc ccgttcagcc gcggcatctg caaatccatc
     1081 aggatgacgt cgggctgcaa ttcggccgcc tgcgtcaccg ccgccgcgcc gtcggccgcc
     1141 tcgcccacca cgttcaacga agcgtcggta gccagcatgg cccgcagccc ttgccggaag
     1201 gcgtcgtgat cgtcacagat gagaatgcgt atcggttcca taccttgcct tatgtttcaa
     1261 tcggaaagcg cgcagtgatg caggtcccgc gcggacggtt cggcagcacg gtcaacgtcc
     1321 ccccgatctc ctcggcccgc tcgcgcatgc cggtcaatcc gatgcccaac tccggctccg
     1381 acggcaaacc cacgccgtcg tcggatattt ccagccgcag gccgtcgttc tcgaaccaca
     1441 gggccagcgt tgccgtctgc gcccgggcat ggcggctgac gttggtcagc gcctcctggg
     1501 cgatgcgata gacggcgacc tcgaccgccg ccggcagcgc ccgcccctct ttcggcaatc
     1561 gcagctcgac gcgcaagccc ggcgcggcga agccgtcggc cagcacttcc agcgctccga
     1621 acagccccaa ctcgtccagc gccggcggtc gcaagccgtg gacgatccgt cgcacctcgc
     1681 tgaccgtcgc ctgggcctgg tcgatcagcc cggtcagcgc ccggtcggcc gccgccgggt
     1741 catcgcggag cagctcgcgg gcggtctcgg cctgcatagt catcgtcgcc agcgccgggc
     1801 cgaggccgtc gtggagatcg cgacgcagga cgcggcgacc ctcttccccg gcggtgacga
     1861 ggtggcggcg cgattcttgc aactcggccg ccagctgatt gttatagagc gccaggctga
     1921 tttgtcgggt cagatcgccc agcagccgct cgtcaatggg cagcggaaac ccggccgtga
     1981 cgcgtggcac aaggcgtaac tcgcccagcg cccggccgtt gtattgcaga ggcagggtga
     2041 cgagcggccc gtcggggcgc gggccgctct cggccgttgc tccctcgctc gtctccagct
     2101 cgacccaggg caaattgagt atctcagtca gcgaatgggc aatggccaga ggcagggcct
     2161 cggccgtcaa ttcggtgtcc agccgggccg aaatgcgact gatggtggtg tagggatcgt
     2221 cgttgttatc ctcgacgaag agcgcgacgc gcggccgcag ccagcgctgc gccggccaga
     2281 ccgacagcaa ggccaggatc atcagaatcc agtcgaccag gccgttccag gcatacggat
     2341 tccccggacc ctcctggaag gtatagccgt tgatcagcca ccacccggcg accagaaagg
     2401 tatagaccag cgtccaccag ccgatgagaa gaagcaccag cagaagcgtc gacaggaacc
     2461 cccgccaggg gatgccccgt cgcccgtcgc ccgccggcaa aggtgattcg cccgtcatag
     2521 tgtcgcctat tatctcttgt cctgtgtaat gtccaatgtc tccccaacaa tcgctatcgt
     2581 attcccaatc gctatcgtat tcgcaaacct tggggcctcg gcggattcaa atccgccgct
     2641 gttaggcgaa acgtgttgaa acgcgtttcc ggcggccgcg tcaaccggct tcagccggtt
     2701 ttatctggca gccacggaat ttattccgtg tgttacaaac gcaatttcat cattcccccg
     2761 gtcgtggccg ggctgtatac agcgccagac tggttagaaa ccacaggccc atccataccg
     2821 cctcgatgat gaacgtcgga tcgaaggtgc cgcgaacgat catcatcgcg ggtatgaaca
     2881 gcccctgggc caacggcgcg aaccgctgcc agccggtcca gcgacgggcc aggacgacgg
     2941 cgattcccgt cagcaacgcg cccaaaagcc cgcccagccc gccgatgggt tgcaggatta
     3001 aaccggcgtc agtgaacagg ctgatgaagc cgccgaggat cagcgttgcc caggcggcga
     3061 tgaacaggcc cagggccagg cggccgaacc ggccttcccc ggccgctcgc gcccgccata
     3121 gcccgatcaa catgatcagc aggccgatca tcccgacgaa gaaccccagt tgattggcga
     3181 cgaagagcgg cccggaagcg ggcgggaaaa gatcgtagcg atattcgacg atcaggttga
     3241 gaaagaagaa agccgcgccg gccatgccga acagggcgaa cgggcgggta ttggtttcgg
     3301 atttcatgat catctccccg atgccgctgc ttttggccgg cgcagcaggg cgacgatgac
     3361 gaagatcgat acgacaatgc cgatgatggc cgatgcccgc gcggcattgt tgggggcgaa
     3421 caggacaccg ggcagcgcgc tcaaaatgcc gacggcgcag agaaaaaggg tcagccagac
     3481 gccccatttc tgccctcgcc aggcgccgta ggcggcgaag agaccggcca cgccactcat
     3541 ggccgccagg ataatgactc cctgggggat gccggcggcg aagccctgat cttccgggcc
     3601 gcccaacagc gggggcatgg tcgccacgac gttgaccagg gagaagagcg cgaacaggat
     3661 gacggctgtt ttgtagaggg gtgaatggtt catgatgtaa ttcctgtagg aaatcaacct
     3721 gtagaaaccg agtttttcgg gaaaaactcg gtttctaatc ggaaaaaccc ggtttctatt
     3781 ccacggccga tcttatgatg cgattatctt cggccggagc aacagaacga cgatgacgaa
     3841 aacggaaacc aaaacggtga cgatggctga aatacgcgca aaatcgctcg gcgcggccgt
     3901 gacgccgggc aacgcgctca acccgttgac ggccgagagg ataatcacca gccatttgcc
     3961 ccatttctgc ccctgccagg cgccataggc ggcaacgaga ccaaccacag ataccagcgt
     4021 ggcgaagatg atgattacct gggggatacc ggcgaccgcg ctctggttat cagggccgcc
     4081 cagcaacgcg ggtatgaatg tcacaacggt gagtacgcaa aggagcgcga acagaatgat
     4141 ggctgttttg taaagaggtg aatggttcat ggctatactc ccggacctga cagatgggcg
     4201 cgatcaagct gccgattggg caagtacccg gatgcgccca cctgtcaggt ctcaatatac
     4261 tggatactgg ctaattctca accgtgaaca tatgcatcat accatgcagc aagtgcggca
     4321 tcggctcttc gcccgcggcc atggccacga ggtccggcaa agggcatacc aactgatact
     4381 gacccggctg caggtccatc tcaacccaca cccgctcgcc cggcgacatc ggcggcatgc
     4441 cggtttccag cacctggatt ggggcgtcgg ccggcggcgg cccgccttcc tcgctgaaca
     4501 tccccaggag ttgttccggc gtcacgtcgg gattataggt cgtcatcacc gccatatgcc
     4561 actggtcgcc ggtgttgatg aactcccaca gtcccttggc cggcacggtg tcgggcatgg
     4621 tgtaggcaaa gtcgaccata tcgaccgtga cggccgtctc cggctcggtc gtaccgacga
     4681 tttcatcggc cttgaagaac accatgaggg tatccgggcg gagttcatcg ccaataccga
     4741 actcccctgt gcgcagatcg gcatagaagg tcttcgtcgc accgggggca atgtcgccaa
     4801 tgacatggac gatgttggcc acttcaaggg cgccgagggc cgcgccggga tcggccgcgg
     4861 ccctggcatt ggcatcgagc acttcctggc tggtgtggcc ttccttcact cttgccagtg
     4921 tcggcgaggc ggggtcgctg ccggcgttgg tgatgtcgaa gcggacgatg ccgccgggga
     4981 cgacttcggg cacattaaag gcgccgtctt tggcctcgac ggtgacgacg gggatttccg
     5041 ccttggccgg ggcctcggtg ggggggacgg ctgtcggcgg aacggccgtc ggcgccggcg
     5101 cggcggcttg ctgcgcgccc gaacccgaac aagcggccgc cagcgccaga acggcgatga
     5161 cgaccagggt caggatcgac caggttcggc gattgatact gtctgcatgt ttcatgatct
     5221 actcctcaaa agggtttgaa ttgatgggca cgaaagtcca tctgaggaat agcatagcgg
     5281 ccggttgtcc tgggtgattg ggaaggtgtc gggaaggttt gggaattacg aattacgaat
     5341 tacgaattac gaattaggaa ggtctcgctt cccattcttc ataagacgcg caggaaggcg
     5401 cccgcttcga ccttgtcgcg cctcctgttc ccctgctccc ctgctccttt tcaccgttcc
     5461 tgattcataa ttccggtata cttcacgcat gattaccagt cttgccaacc cgcaggtcaa
     5521 atatgttcga cgcttgcaat ccgaccggcg ctttcgcgcc aacgagcggg ccttcgtcgt
     5581 cgagggtacg cgctggatgc gcgatctggt tgccgcgcga gccgtgccgc gggcgctgtt
     5641 cgccacggcg gcgtggctgg agagcaatga ggggcgcggg ctgtcggcga cgttgggcgc
     5701 ggccgtggcg gtgtcggatg aggtcatggc ggccatgagc gacacggaga ccgcgccggg
     5761 cgtgctggcc gtgctgccca ttgtgccgcg gccgtggccc gacgcgccgg cgctggtagt
     5821 gatcctcgac gcggtgggta accccggcaa tctggggacg atcctgcgcg cggccggggc
     5881 ggcgggggcc gacggggtgc tgctggggcc gggctgcgtc gatgcgtata acccgaaggt
     5941 cgtccgcggc agcatggggg cgctgctgcg gctgccggtg cgggcggcgt cttgggccga
     6001 tatcggcgcg ctaacggccg ggctggatgt gtggctggcg gcggcggacg gcgcgacggc
     6061 ctacacggct gtggattggc ggcggccgtc ggcgattctc atcggcggcg aggcccacgg
     6121 agcgggcgcg gccgccgggc aactggcgac cggccgggtg accatcccca tgcacgacgc
     6181 gaccgaatcg ctcaacgccg c
//
LOCUS       DAOMQG010000185         6196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5401_length_6196_cov_9.786028, whole genome shotgun sequence.
ACCESSION   DAOMQG010000185 DAOMQG010000000
VERSION     DAOMQG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_6196_cov_9.786028"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..413)
                     /locus_tag="PLC06_10530"
     CDS             complement(12..413)
                     /locus_tag="PLC06_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaoD/DsrE family protein"
                     /protein_id="HRO90923.1"
                     /translation="MKVAYVFAHPRAASYKLGRMILPQLEAGTHGVDVVGMFFFDDNT
                     FVLREGDPIGERLAKVAAEKGMLLMMCDMCALERGLAAGEPRWCDAQGMGRAEPGECH
                     VVDTVAGVQVGCFPDLYAALAGNMPDQVITL"
     gene            520..1332
                     /locus_tag="PLC06_10535"
     CDS             520..1332
                     /locus_tag="PLC06_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HRO90924.1"
                     /translation="MSASNDEVIVFYHLPKSAGTTLNRILRHNYRRDEMAESGPNTQE
                     FVAELKSWPPERLAAIRFLQGHYPWGLHEMLPQRARCFTILRDPVERVLSHFYHAQRQ
                     PDYFLYDLIQDNNGSLPKLLESGIPLMMNDGQVRLLSGVYADPHMGEVTETMLATAVA
                     NLRSCAVVGITEQFDLSLVLLQRAFGWRFIGYKRPVNVGHNRPPAREIDAEALEAVRH
                     YNRMDALLYEEAQRLMAQQAADAGRTLGVRLMALNIQKKIPDRWIGRLPGVY"
     gene            complement(1336..6162)
                     /locus_tag="PLC06_10540"
     CDS             complement(1336..6162)
                     /locus_tag="PLC06_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90925.1"
                     /translation="MKKIAPVLSLALLLIAAFVLPAAVRPSFVAAQTPATLIDRAGGT
                     YNGIEVILAADAAPAPGDTVTLTLTARPLRAAPNVYVEWALPDGGELLGGSATETLGP
                     VGIGETVTLTRQARFPAGVHQVSAKAFYFPNRATSLAALGVLFFDVRPDSPTAATLDP
                     RTPVYSPPTARPTIDKSGLATSGPIDRAADGCFNVHGILTRENRMPITSVVSEPGLPP
                     GTPPLYAGSYQDQLGSAVPVHHILVEMREEDTISDDSYGHTVTDANGKFTFNFCDDDG
                     FLNDELELYFRVCAEVRDGNSLIATIKNLDEQKLYCWDSGTIDSEGGDVDFDLTVYKI
                     GQTEASVFNIADSLYWAWRFWNNNAANSPVMDRAVTAYWQGGKGSGSFYNDDRTALVI
                     AGDSSNTDEWDDSVIIHEWGHFADHQFSCNQNPGGAHSLPGVNAGTNATRLAWGEGYP
                     DYYQSVARTLMPGSSAVNFYVDPSGPTVDLENMRFVTAADTDEGAVAAMLWDFHDTTN
                     DSQDTVSHGHATTQRVYTTGDFKNNTQCDARRFIEVWRNLGLPTDAATAATIVQNVNV
                     TLASLPPAPAISRSVDQYSVDQYSVDQLDSGSVSNTDSPNTDSLTTDPLTTDPLTTAS
                     LTAAPPFGFRWWDQVTMVVDNSSSMAASGKINTVKTIIGEQVSDLAPAPQGTEFNIYT
                     FNAGNPAIRPAAEGKFFANTVMPAVNGIVANGADAGCPVPGLGALSQAVINKFDGEAW
                     LYTDGDSADTIRPETLRQTLNNQRLRASIVLLGGCGSPARKQPDVTGQERTYLGLAAD
                     GSQSSGVVPYMLTSLLSGGQFIYAAPDQLANAVDMVRAQLSHTAGAGKWSDYVSTGYT
                     YRWDRLEPWEYQWFPGNGLGTDKGQIGSDPLPVKLDTPFPFYGVETSVVDVSEDGYIV
                     MNPCLVDPQFCQFLFTQYVELLDTDLSWVYINKPPSLPPNAPAATNACGLPDTWGPQV
                     RVYYANFAFEWMILSTEGWANYGSGSTGCRAYQVWLNHKTGEIRFQYALLRQEAATAE
                     IGLVDSLFFTGNNVIVSKNDLGGATNSMGYKFTPAPPQPAKTYEVEVDPLIESIIFMQ
                     TGYSGDFAPMAVTRPDGTAVNCADTANVKCITMNNKPGDNMVQFVQVNTNGTSGIYKA
                     VVAVGPSGSGTFSFNALAASELTASGPGNHTLALKTAHPFVVDLGRTVDGNQVQGWLQ
                     TPAGLPFGHPFTLYDDGAHGDDAAGDGRFGSGPFTAPGAGAAYLWVEGTIGGTTFRRS
                     DPAPYNFQPLVVTAEPGYVEGFYAAGAYVGYVATNQDSVEHCYGVEFTVPAGWWVNSP
                     YINSGICISAGQSGYPYTYAGRDLSDETLGEVGEVTMTLTEVDEGAITGSAAARVALF
                     RPVAAIAFDNRQVGPIRPNGADTVELTLNLYDDLGQSVGYSGPFSGELTVTGGTYTLP
                     VGMYEDGRLRILFTAGSAPGIATLSALAEGGNMAETTIEMAEPTAADIQLTASPTNLS
                     NAGQSALTVTVRDLYGDPSPGETVRLSISDDAGDQGKINGGETFEGATNKNGQMKATF
                     IKTAGGKGTVVIRAELLEPGDPNDSDRKVLRETSVTLYLSGAPAGGQRIFLPTVLR"
BASE COUNT         1132 a         1896 c         2029 g         1139 t
ORIGIN      
        1 ctggatagca gctacaaagt aatcacctga tccggcatat tccctgccag cgccgcatag
       61 aggtcgggga agcagccgac ctgcaccccg gcgacggtat cgaccacgtg gcactcgccc
      121 ggctcggcgc ggcccatgcc ctgggcgtcg caccagcgcg gctccccagc ggccagcccg
      181 cgctccagcg cgcacatgtc gcacatcatc aacagcatcc ccttctccgc ggcgaccttc
      241 gccagccgct cgccgatggg gtccccctcg cgcaggacga aggtattgtc gtcgaagaag
      301 aacatgccga cgacatcgac gccatgcgtc cccgcttcca gttggggcag gatcatgcgg
      361 ccgagcttgt agctggcggc gcggggatgg gcgaatacat aagcaacttt catggtatct
      421 cctgaattga atagtaataa gcaggctcca tcataccgcc ggaagtgaaa tatgcctaaa
      481 cgggaaaatg gggcacaatg gggcatccgg ttatatgata tgtctgcatc caacgacgaa
      541 gttatcgttt tctaccacct gcccaagtcg gcggggacga cgctcaatcg catcctgcgc
      601 cacaactaca ggcgcgacga aatggcggag agcggtccca acacccagga gttcgtcgcc
      661 gaactgaaga gctggccccc ggagcgactg gcggccatcc ggttcctgca agggcactac
      721 ccctggggcc tgcacgagat gctgccgcaa cgggcgcgct gcttcaccat cctgcgcgac
      781 ccggtggagc gcgtgctctc ccacttctac cacgctcagc gccaacccga ctacttcctc
      841 tacgacctca tccaggataa caacggatcg ctgccgaagt tgctggagag cggcatcccc
      901 ctgatgatga acgacgggca ggtgcgactg ctgtcgggcg tctatgccga cccccatatg
      961 ggcgaagtga ccgagacgat gctggcgacg gccgtcgcca atctgcggtc gtgcgcggtg
     1021 gtgggcatca ccgagcagtt cgacctgtcc cttgtgctgc tgcagcgggc tttcggctgg
     1081 cggttcatcg gctacaagcg accggtcaac gtcggccaca accggccgcc cgcgcgggag
     1141 atcgacgcgg aggcactgga ggccgtgcgg cactacaacc gcatggacgc gctgctctac
     1201 gaagaggccc agcggctgat ggcccaacag gcggccgacg cggggcgcac gctgggggtg
     1261 cggctcatgg ccctcaacat ccaaaagaag atacccgaca ggtggatcgg ccgcctgccg
     1321 ggtgtctact agtcgctacc gcagcaccgt cggcaggaag atgcgttgcc ccccggccgg
     1381 cgcgccgctc agatagagcg tcacgctcgt ctcgcgcagc actttcctgt cggaatcgtt
     1441 ggggtcgcca ggctcaagca actcggcgcg aatgaccacc gtccccttgc cgccggcggt
     1501 cttgatgaaa gtggccttca tctgcccatt cttgttcgtc gctccttcga acgtctcgcc
     1561 gccgttgatc ttgccctggt ctcccgcgtc gtcgctgatg ctgaggcgca ccgtctcgcc
     1621 cggcgaagga tcgccgtaga ggtcgcgcac ggtgacagtc agcgccgact ggccggcgtt
     1681 gctcaggttg gtgggtgagg ccgtcaactg gatgtcggcc gccgttggct cggccatctc
     1741 gatggtcgtc tcggccatgt tgccgccctc ggccagcgcg ctgagcgtgg caatgccggg
     1801 cgcgctgccg gcggtgaaga ggatgcgcag gcggccgtct tcgtacatgc caaccggcag
     1861 cgtgtacgtg cccccggtca cggtcagttc accgctgaac gggccggagt agcccacgga
     1921 ttgacccagg tcgtcgtaga gattgagcgt caactcgacc gtgtccgcgc cgttgggccg
     1981 gatggggccg acctggcgat tgtcgaaggc gatagccgcc accggccgga acagcgccac
     2041 ccgggcggcg gccgagccgg tgatggctcc ctcatcgact tcagtcagcg tcatcgtcac
     2101 ctcgcccact tcgccaagcg tctcatcgct caggtcgcgc ccggcgtagg tataggggta
     2161 gcccgattgc cccgcgctga tgcagatgcc ggagttgatg tagggcgagt tgacccacca
     2221 gccggcggga acagtaaatt cgacgccata gcaatgctcc acgctatcct gattcgtcgc
     2281 gacatagccg acatacgcac cggcagcata aaagccctcg acataccccg gctcagcggt
     2341 gacgaccagc ggctggaagt tatagggggc cgggtcggag cgcctgaacg tcgtgccgcc
     2401 gatagtgccc tcaacccaca ggtaggccgc gcccgcgcca ggggcggtga acgggccgga
     2461 gccgaagcgg ccgtcgcccg ccgcgtcgtc gccgtgggcg ccgtcatcgt agagggtgaa
     2521 gggatggccg aaaggtagtc cggccggagt ctgaagccaa ccctgcacct gattgccgtc
     2581 gacggtgcgg cccagatcga ccacgaaggg atgagcggtt ttcaacgcca gcgtgtggtt
     2641 accggggccg gaggcggtca gttcgctggc ggccagcgcg ttgaaggaga acgtgccgct
     2701 gccgctgggg ccgacggcca caaccgcctt gtagatgccg ctcgtgccgt tggtgttcac
     2761 ctgcacgaac tgcaccatgt tgtcgcccgg cttgttattc atggtgatgc atttcacgtt
     2821 ggctgtgtcg gcgcagttga cggccgtgcc gtcgggccgg gtgacggcca tcggcgcgaa
     2881 gtcgccgctg tagccggtct gcatgaagat gatgctctca atgagcggat cgacctcgac
     2941 ctcgtaggtc ttggccggct gcggcggcgc gggggtgaac ttgtagccca tgctgttggt
     3001 cgcgccaccg agatcgttct tgctgacgat gacgttgttg ccggtgaaga acagggagtc
     3061 caccagaccg atctcggccg tggccgcttc ctgccgcagc agcgcatatt ggaatcgtat
     3121 ttcgcccgtc ttatggttca gccacacctg ataggcgcgg caaccggtgc tgccgctgcc
     3181 gtaattggcc catccttctg tggacaagat catccactcg aaggcgaagt tggcgtagta
     3241 aacacgaacc tgtggccccc aggtgtcggg taacccacaa gcgttggttg cggcgggcgc
     3301 gttgggcgga aggctgggag gtttgttgat atagacccag gacaagtcgg tatccagcag
     3361 ttccacatat tgagtgaaaa ggaactgaca gaattgcgga tccaccaggc agggattcat
     3421 cacgatgtag ccgtcttccg agacatccac aacagacgtc tccaccccat agaacgggaa
     3481 aggtgtgtcg agtttgacgg gcaaggggtc gctgccaatc tgccccttgt cggttcccaa
     3541 cccgttgccc ggaaaccact ggtattccca cggttccagt cgatcccagc ggtaggtgta
     3601 gccggtgctg acgtagtcgc tccatttgcc ggcgccggcg gtgtggctca gttgggcgcg
     3661 caccatatcc acagcgttgg ccagctggtc gggggcagcg tagatgaact ggccgccgct
     3721 gagcagcgag gtcagcatgt agggcacgac gccgctggat tgcgagccgt cggccgccag
     3781 ccctagatag gtgcgctcct ggccggtcac gtccggctgc ttgcgggcgg gcgagccgca
     3841 gccgccgagc aggacgatcg acgcgcgcaa ccgctggttg ttgagcgtct ggcgcagcgt
     3901 ctccgggcgg atcgtatcgg cactgtcgcc gtcggtatag agccacgctt caccatcgaa
     3961 cttgttgatg acggcctgcg atagcgcgcc taggccgggc acggggcaac ccgcgtccgc
     4021 gccgttggcg acaatgccgt tcacggccgg catgaccgta ttggcgaaga acttgccttc
     4081 cgccgccggc cggatggccg ggttcccggc attgaaggtg tagatgttga actcggttcc
     4141 ctgcggggcc ggagccagat cgctcacctg ctcgccgatg atcgtcttga ccgtgttgat
     4201 cttgcccgaa gcagccatgc tgctgctgtt atccacaacc atcgtcacct gatcccacca
     4261 gcggaagccg aaaggcgggg cggcggttag ggaggcggtg gtcagtgggt cagtggtcag
     4321 tgggtcagtg gtcagtgagt cagtgttcgg tgagtcagtg ttcgatactg atccactgtc
     4381 caactgatct actgaatact gatctactga atactgatct actgatctac tgatagccgg
     4441 tgccggcggc agactggcca gggtgacatt gacattctgc acgatggtcg ccgccgtggc
     4501 cgcgtcggtc ggcaggccca aattgcgcca tacctcaatg aagcgacggg catcgcattg
     4561 ggtattgttc ttaaagtcgc cggtggtata gacccgctgg gtggtcgcgt ggccgtggct
     4621 gaccgtgtcc tggctgtcgt tggtcgtgtc gtggaaatcc cacaacatgg cggcgacggc
     4681 gccctcgtcg gtgtcggccg ccgtcacgaa acgcatgttt tccaaatcca ccgtcgggcc
     4741 gctgggatcg acgtagaagt tgacggccga cgagccgggc atgagcgtcc gggccacgga
     4801 ctggtaatag tcgggatacc cttcgcccca ggccagccgc gtggcattcg tacccgcatt
     4861 aacacccggc agcgagtgcg cgccgccggg attctggtta cagctaaact gatggtcggc
     4921 aaaatggccc cattcatgga tgattactga atcatcccac tcatcggtat tggacgagtc
     4981 gccggcgatg accagggcgg tccgatcgtc gttatagaat gagccgcttc ccttgccacc
     5041 ctgccagtag gcggtgacgg cgcgatccat aaccggcgag ttggccgcat tgttattcca
     5101 gaagcgccac gcccagtaga gcgaatcggc gatattgaaa acagaagcct cggtctggcc
     5161 aatcttgtag actgtcagat cgaaatcgac atcgccaccc tcggaatcga tagtgcctga
     5221 gtcccagcag tacaacttct gctcgtcgag gtttttaatc gtggcgatca ggctattgcc
     5281 gtcgcgcact tcagcacaaa cgcggaagta cagttccagt tcatcattga ggaagccgtc
     5341 gtcgtcgcaa aagttgaagg tgaatttgcc gttggcgtcg gtcacggtgt ggccatagga
     5401 gtcatcggaa atagtgtctt cctcgcgcat ctcgaccaga atgtgatgga caggcacggc
     5461 cgatcccaac tgatcctgat agctgccggc atagagcggc ggcgtcccag gcggcaggcc
     5521 gggttcagac acgaccgacg tgatcggcat acgattctcg cgagtcagga tgccgtggac
     5581 gttgaaacag ccgtcggccg cgcggtcgat tgggccgctc gttgccagac cggacttgtc
     5641 gatagtaggc cgggcagtgg gtggggagta gaccggcgtg cgcgggtcaa gggtcgcggc
     5701 agtcgggctg tcggggcgca catcgaagaa caacacgccc agcgcggcca gcgaggtcgc
     5761 ccggttgggg aagtaaaacg ccttggcgct gacttgatgg acgccggcgg ggaaacgggc
     5821 ctgccgcgtg agtgtgaccg tttcgccgat gccgacaggg ccaagcgttt ccgtagccga
     5881 gccgccaagc agctcaccgc catcgggcag cgcccattcg acatagacgt tgggcgcggc
     5941 gcgcagcggg cgagcggtca gggtcagggt gacggtatcg ccgggcgcgg gggcggcgtc
     6001 ggccgcaagg atgacctcga tgccgttata tgtgccaccg gcgcggtcga tgagggtggc
     6061 cggggtttgc gccgcgacaa atgaggggcg cacagcagcg ggcaagacaa acgccgcgat
     6121 gagcaggagg gcaagggaga gaacaggagc gatcttcttc atggttcacc ttgggctata
     6181 acggccctgt tcggtt
//
LOCUS       DAOMQG010000186         6167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5432_length_6167_cov_10.102421, whole genome shotgun sequence.
ACCESSION   DAOMQG010000186 DAOMQG010000000
VERSION     DAOMQG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5432_length_6167_cov_10.102421"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3215)
                     /locus_tag="PLC06_10545"
     CDS             complement(<1..3215)
                     /locus_tag="PLC06_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO90926.1"
                     /translation="MKITKQIGGLFLAMMVAGWLLAGTLGLFGVDPTSAVAAPVCDWE
                     VYPIGENPLQTAINNAASGQTVCVFGGIYHEIVRVPVGKTGLTLIAAPGELPVIDGQK
                     VLPGGMPADRFRALVELSGSGTTFDGFEVRYSSAGGIDITTSNVVVRNNSVHDNWSTG
                     INARSNSQIGGILIENNQVFNNVRRAQSIPVIYRGVRGAGNGAADWVFDSSTYWDAPF
                     WTGVNADLPEQWLNGVAMTFNDDGATSRVYAGSPRAGRVGTIGAGYSSSGQQFSYSGA
                     DILFHEPATNQWTLYFDGEPNGIPQNSVIDAFQIENTAPISEPWPCDTCAPMLLSFTA
                     TVSLPITEGVLTPTITQTIIGPSDLVYFRPAAMNATRISNGYYTLHKRAADLVGLPEG
                     ANIDALDRAPDGRLLISLNVDDILTNPDGSTINAERENLVAYDEATGYWSLFFDGDQI
                     PYNPFQAEDLTAAWVDDDGNIYISGNPVGGSALTLINTLDSTARGNTVFNNYGEGIVS
                     DRFSVRATLEDNVAYDNLHANLYLNSTTDPLVRRNLVYCTNDRRFWRKGSSIAYHPSP
                     GIQIRDEIFDVGTPASSGQVIINNIVVGCSNNFGVTAQAPGDGGGLNNALVANNTFVN
                     ARGDTAEGINNIELSSGASYANSRFINNLILQNTPGAILRVQGTGANFSTFTVANNLY
                     SVAPPTTWFFGEAGRVVDNPMLNASTPPLPVMGVSPDPEDYRLTYNSPALDAGQTLAE
                     LTDDFFATVRVGNGMPDIGAHELPHIGRIIVTQATAPTGSAQQFDFTADFSPTGFSLV
                     DGGQYDSGVLEGGTYALAVTPLEGWTMTAGCSDGSPIGAISLGPNETVICAFTGVQKA
                     QLVIDHIIEPADNPLLVDFTLSPGETFQMGSESRTFVIEPGAYSLVAAAPQDWQQEAA
                     CDNGDAPGALTLEAGEMVVCTFTHRQPGHIIVTKQTDPPGTPSVFQFVASYGDFSLSH
                     GQSHDSGPLQPRTIYSVAEMPLAGWAISSATCTGDDDGTDPAAITLDPGEVVSCVFIN
                     SRTTSGPLATFYLTTPKAGSVQGLAYSPGDILAYDNATATWS"
     gene            complement(3400..>6167)
                     /locus_tag="PLC06_10550"
     CDS             complement(3400..>6167)
                     /locus_tag="PLC06_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO90927.1"
                     /translation="NRMKRTDPKWIAMLALLALLALVGGAAAGNSGNQSLIAPVINDA
                     ADCTTIINSGQSIQAAINTAQSGHVICVHAGTYHEQLMFRINKAGITVIAYPGEHPIL
                     DGRNVIPAGKFQGLVQINADNVTVDGFEVRDSNARGVIVGHPTNGTASLRNAVIRNLV
                     VHGSYDSGININGSDQYHLVDILVENNVVYNNLRKNAGGADGGSALTFVDVDDSIARG
                     NTIYNNFGEGLVADRWTNNLIFEDNISFDNKNANIYLSTTTSPLVQRNLVFCTDDRDW
                     WRDDKGKPPPGIMMRDEDYEGQTEKPPASTDQVVINNIVVGCGINFGVSTQFNGGGLN
                     GALIANNTFVNARGDSSVNNVLFEGDANYRNSSFVNNLILQAYDTSVTPATILTSLGD
                     PDLSTFTLGNNLYSHPPSSKWPANEPGRVVADPKLVNPTMPVKGSIPDANGYALQSNS
                     PAINAGRAVARVTEDFFGQSRGGVLDIGADETGGGPPPTTGQVVVTVATTPDQSAQVF
                     SFTAGYTGNPFELTDGETHSSGQLAPGTYSVAMTPVDGWTTFATCSDGSAPGAINLAA
                     GETVTCTFNNEEQAAEVTRIIVLKTTIPAGEAQVFQFASNFAGAFGLAHGGQKQTDVV
                     AGAYMVTESVPEGWIQASATCNDGSTPDNINVSEGETVTCTFVNQKVIPPLDAAIYVT
                     TTADGTVGDITFSKGDILAYDGSWSMYFDASDVGIMNNLNDFAIMPDGSLLIAISGRT
                     TLNTNNGSFKLQLWDIARFVPSSLGDNTSGYFEMYFDGSDVGLTKASEKIDALAIQPN
                     GTLLISTYGTASVPNGALVIKAQDEDLLAFQPSSTGDNTQGVWSLALDGSTIPGLGKE
                     DIDAVWRDGNSGAYYLTMTTDFIIGGVTGTPGTILAVTPGGTVTHYWNAADAGLTGSI
                     TGIHIVP"
BASE COUNT         1279 a         1769 c         1799 g         1320 t
ORIGIN      
        1 gaccatgtgg cggtggcgtt gtcataggcc aggatgtctc ccggcgaata ggccagcccc
       61 tggacgctcc ccgccttcgg cgtcgtcagg tagaacgtcg ccaacgggcc ggaagtcgtc
      121 cggctgttga tgaagacgca cgacaccacc tcgccgggat cgagtgtgat ggctgccgga
      181 tctgtgccgt cgtcatcacc cgtgcaggtc gcgctcgaaa tggcccaacc ggccaacggc
      241 atttcggcca cgctgtagat cgttctcggc tgtagtggcc cggagtcatg actctggccg
      301 tggctaaggc tgaagtcgcc gtaactggcc acgaactgaa agacgctggg ggtgccgggg
      361 ggatcggtct gcttggtgac gatgatatga ccaggttgcc gatgggtaaa agtacagacg
      421 accatctcgc ccgcttcaag tgtcaacgcg ccgggtgcat cgccattgtc gcaagcggcc
      481 tcctgttgcc aatcctgggg cgcggccgca acgagcgagt aagcgcccgg ttcgatgacg
      541 aatgtccggc tctcgctgcc catctggaac gtctcgccgg gtgaaagcgt gaaatcaacc
      601 agcaacgggt tatcggccgg ctcgattatg tgatcgataa caagttgggc tttctgcacg
      661 ccggtgaacg cgcagatcac ggtttcattg gggccgagtg aaatcgcccc gatcggcgat
      721 ccgtcgctgc agccggccgt catcgtccag ccctcgaggg gagtgacggc gagagcatag
      781 gtgccgccct ccaaaacacc ggagtcatat tgcccgccat cgaccagcga gaaacccgtc
      841 ggcgagaagt cggccgtgaa atcgaactgt tgcgctgatc cggtaggggc cgtggcctgg
      901 gtcacgatga tccggccaat gtggggcagc tcatgagcgc cgatgtcggg catgccgttg
      961 cccacccgaa cagtagcgaa gaaatcgtcc gtcagctccg ccaacgtctg ccccgcatca
     1021 agagccggag agttataggt caggcgataa tcctccggat cagggctgac gcccatcaca
     1081 ggcaaaggtg gggttgaagc gttcagcatg ggattatcga cgacgcgccc cgcttcgccg
     1141 aagaaccagg tagtcggggg cgcaacgctg tagaggttgt tcgcaacagt aaacgtggag
     1201 aaattggctc ccgtcccttg aacccgtaga atcgcgccgg gggtgttctg gagaatcaga
     1261 ttattgataa agcgcgagtt ggcataggaa gcgccgctgc tcagctcaat attattgatt
     1321 ccctcggccg tatcgccgcg cgcattgaca aatgtattgt tcgcaaccaa cgcattgttt
     1381 aatccgcccc catcgccggg cgcctgcgcg gtgacgccaa aattattgct gcaacccacc
     1441 acgatgttat tgatgatcac ctgccctgag ctggccggcg tgccgacatc aaatatctca
     1501 tcccgaatct gaatgcccgg gctgggatga taggcgattg acgatccctt tcgccagaat
     1561 cgccgatcat tggtgcaata aacgagattg cgccggacaa gcggatcggt cgtcgaattc
     1621 agatagagat tggcgtgcag gttgtcatag gcgacattgt cttccagcgt cgcccgaacg
     1681 gagaaccgat cggagacaat cccttcgcca taattattga agacagtatt gccacgggcc
     1741 gtggaatcga gggtattgat caacgtcagg gcgctgccgc ccacaggatt accgctaata
     1801 tagatgttgc catcatcatc gacccaggcc gccgtcagat cttcagcctg aaacggattg
     1861 tacggaatct gatccccgtc aaagaacaat gaccagtatc ccgtagcctc atcataggcc
     1921 accaggtttt cccgctcggc gttgattgtg cttccgtcgg gattggtcag gatatcatcg
     1981 acattcaggc taatgagcaa acgcccatcc ggcgcgcggt caagcgcgtc gatgttggcc
     2041 ccttccggca gcccgaccag gtcggcggcg cgtttgtgga gcgtgtaata gccgttactg
     2101 atgcgggtgg cattcatcgc tgccggccga aaatagacga gatcgctcgg cccgattatc
     2161 gtttgggtga tcgtcggggt caggacgcct tcggtgattg ggagggaaac ggtcgcagta
     2221 aagcttagca gcatgggcgc gcaggtatcg cacggccaag gttcgctaat cggcgcggtg
     2281 ttctcgatct ggaaggcatc gatcacactg ttttgtggaa taccatttgg ctcgccatcg
     2341 aagtagagcg tccattggtt tgtcgccggc tcatggaaaa ggatatcagc gcccgaatag
     2401 ctgaattgtt ggccgctact gctatatccc gctccgatgg tgcctacccg accggcgcga
     2461 ggcgaaccgg cataaacgcg agaggtagcg ccatcgtcgt tgaaggtcat ggcgacgcca
     2521 ttgagccatt gctccggcag atcggcattc acaccggtcc agaaaggcgc atcccaatag
     2581 gtcgaggagt caaataccca gtcggccgcg ccgttccccg cgccgcgcac cccccgatag
     2641 ataacgggga tggactgagc gcgtcgaaca ttgttgaaaa cctggttatt ttcgatgagg
     2701 atgccgccga tctggctgtt gctgcgcgcg ttaatacccg tgctccagtt atcatggacg
     2761 gaattgttgc gcacgacgac attgctggta gttatgtcaa taccgccggc gctggaatag
     2821 cgcacctcga agccgtcgaa tgtcgtcccg ctgccgctca attccaccag cgcccggaag
     2881 cgatcggcgg gcatgccacc gggcaagacc ttctgcccgt cgatgaccgg caactcgccc
     2941 ggtgcagcga tgagggttag gccggtcttg cccacgggaa cgcggacgat ttcgtggtag
     3001 attccaccga agacacagac cgtttgcccg gaagcagcgt tattgatggc cgtttggagc
     3061 gggttctcgc cgatggggta aacctcccag tcgcagacag gagccgcaac ggccgatgtt
     3121 gggtcaacac caaataaacc cagggtgccc gccaaaagcc agccggccac catcatggcg
     3181 aggaataacc caccgatttg cttcgtaatt ttcatatacg ccatcatcgt gcgccatcgc
     3241 gggtatagag caagaattga ttgtaagcga gaaaccttac aaccattatt actcgcgttg
     3301 tcatgttatt cttgccggca atagacaaaa ggccggagac gatatccccg gcctttaaat
     3361 ctatccgtga tccttacatg gtcatgcgtg gctagtgaat caaggtacga tatgaattcc
     3421 tgtgatcgac cccgtaagcc cggcatcggc cgcgttccag tagtgcgtca ccgtgccgcc
     3481 aggcgtaacc gccaggatgg tgccgggtgt tcccgtcacg ccgccaataa tgaaatcagt
     3541 cgtcatggtc aggtagtagg ccccggagtt gccatcgcgc cagacagcgt ctatgtcttc
     3601 cttccccagg ccggggattg tcgatccgtc gagggccagc gaccacacac cctgggtgtt
     3661 atcgcctgtg ctgctcggct ggaaagccag caagtcttca tcctgcgcct taatcaccaa
     3721 tgcgccgttc ggcacactgg ctgtgccgta ggtgctgatc agcaacgtgc cgttcggttg
     3781 aatcgccagt gcatcgatct tctcgcttgc cttggtcagg ccaacgtcgg agccatcgaa
     3841 gtacatctcg aaatagccgg acgtgttgtc ccccaggctc gacggcacga accgggcgat
     3901 atcccacagt tgcaacttga acgatccatt gttggtgttg agcgtggtgc ggccgctgat
     3961 ggcgatgagg agtgagccgt cgggcatgat ggcgaagtcg ttgaggttgt tcatgatgcc
     4021 gacatcagaa gcgtcaaagt acatcgacca actgccgtca taggccagga tgtctccctt
     4081 ggagaaggtg atgtcgccaa ccgtcccatc agcggtcgtc gtcacataga tagccgcatc
     4141 aagcggggga atcacctttt gattgacaaa cgtgcaggtc acggtctcgc cttcactcac
     4201 gttgatattg tccggtgtac ttccgtcgtt gcaggttgcg cttgcctgga tccagccttc
     4261 cggcacagat tccgtcacca tataggcccc ggccaccaca tcggtctgct tttgcccgcc
     4321 atgtgccagg ccaaaggctc cggcgaagtt actggcgaac tggaaaactt gcgcctctcc
     4381 ggcgggtatc gtcgtcttaa ggacgataat tcgcgtaacc tcggcggcct gctcctcatt
     4441 gttaaacgtg caggtaactg tttccccggc cgccagatta atcgcgcccg gcgcggatcc
     4501 gtcgctgcaa gtggcgaatg tcgtccaccc gtcgacgggc gtcatggcca cgctataggt
     4561 acccggcgcc aattggcccg aggaatgggt ctccccatcc gtcaattcga acgggttgcc
     4621 ggtatagccg gcggtaaaac tgaacacctg ggcagactgg tcgggcgtag tggccacagt
     4681 gacgacgacc tggccggttg tgggcggggg gccgccgcca gtctcgtccg cgccgatatc
     4741 cagaacccca ccgcggctct gaccgaagaa atcttccgtc accctggcca cagcgcgccc
     4801 ggcgttgatg gccggcgagt tcgactgcaa ggcatagcca ttggcatcgg ggatgctacc
     4861 cttcacgggc atggtcggat tgaccagctt cggatcggcc acaacgcggc caggctcgtt
     4921 ggccggccat ttactcgatg ggggatgact atacaggtta ttccccaacg taaaggtgct
     4981 caaatcaggg tcgcccaaac tggtcagaat cgtagcggga gtaaccgacg tatcatacgc
     5041 ttgcaggatc aggttgttga cgaagcttga gtttctgtaa ttcgcgtcgc cttcgaacag
     5101 gacgttattg acgctgctat cgccacgcgc gtttacaaag gtgttgttgg cgatcaacgc
     5161 gccattcaac ccgccgccat tgaactgggt gctcacaccg aagttgatgc cgcaacccac
     5221 gacgatattg ttgatcacca cctggtccgt gctggccggg ggcttctcgg tctgtccttc
     5281 gtaatcctca tcgcgcatca tgatgccggg cggcggtttg cccttatcgt cgcgccacca
     5341 atcgcgatcg tcggtgcaga aaaccagatt gcgttggacc aaggggctgg tcgtagtcga
     5401 caggtagatg ttggcgttct tgttatcaaa cgagatattg tcttcaaaga tcaggttgtt
     5461 ggtccatcgg tcggccacca atccttcgcc gaaattgttg tagatcgtgt tgccgcgagc
     5521 gatgctgtca tccacatcaa cgaatgtcaa cgcgcttcca ccatctgcgc cgccggcatt
     5581 cttgcgcaaa ttattgtaga cgacgttatt ttcaaccagg atatcgacca gatggtactg
     5641 gtcggaaccg ttaatgttga tccctgaatc ataactgcca tgcacaacca ggttacggat
     5701 gaccgcgttg cgaagggatg ccgtgccgtt cgttggatgg ccgacgatga cgccgcgcgc
     5761 gttggaatcg cgaacctcaa acccatcaac ggtcacatta tcggcattga tctgcaccag
     5821 gccctggaat tttcccgcgg gaatgacatt gcgcccgtcc agtattggat gctcaccagg
     5881 ataggcgatc acggtaatcc ccgccttgtt aatcctgaac atcaactgtt catgataggt
     5941 ccccgcgtgc acgcagatca catggccact ttgggccgtg ttgatggccg cctgaatgct
     6001 ttggccggaa ttgattattg ttgtgcagtc cgcggcatcg ttaatcactg gggcaattaa
     6061 ggattgatta cctgaattgc cggccgctgc gccgccgaca agggcgagca gggccaggag
     6121 agcaagcatg gctatccatt tggggtcagt tcgtttcatt ctgttac
//
LOCUS       DAOMQG010000187         6165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5434_length_6165_cov_6.802128, whole genome shotgun sequence.
ACCESSION   DAOMQG010000187 DAOMQG010000000
VERSION     DAOMQG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5434_length_6165_cov_6.802128"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1584
                     /locus_tag="PLC06_10555"
     CDS             241..1584
                     /locus_tag="PLC06_10555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO90928.1"
                     /translation="METPTQRPLDRFRRGLATYMANIRLFSRNVWMVLFYGIGMGLTF
                     GVVMFTFNFYVLSLGPAFDEGFLGVLQSAASFATIAMAIPAAWIAQRYPPKWVMIAGG
                     LIGAIAYMGIVLYPTREGLVVFRMLAGVSMSLGMVAGAPFLMANTGAAERQWAFSFQA
                     GLSTVASFFGNLLAGILPGRLGALVGVGPTDTLAYQLALGLMIALSLLSIIPLLLIRS
                     AAAPAKANELPWVQLRRHRRLFAIVLLPSLLIGLGAGLMQPFMNVYFRNVYGKPDAAI
                     GIVFALGGLAMAIGQFAGPPLADRYGKIRTVMLTQALSIPFLVTLGLGAWLVTAGLAR
                     AELFFFLAGIAYIFRLALMNLSNPVYQTFVLERVPEQAQALAASLTALVFQVGWFVMP
                     QISGQIQVTHGPGGFAYIFAGVTMFYIVAIATEKWFFGGEDKARAAGRCELQAMN"
     gene            complement(1683..3371)
                     /locus_tag="PLC06_10560"
     CDS             complement(1683..3371)
                     /locus_tag="PLC06_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRO90929.1"
                     /translation="MPLTLNNFKREIPATILTRGREYFRAGQVVDLEEDGDDGWAAVV
                     QGTAPYEVRIKRLGDKSLEVYCNCPYSDGPYCKHVAAMLYAIEEVFPEQFEGKRRKAT
                     TKRTTKLDRLRAALDAAPQQQLVDIVMETARGDREFQSWLLMQLGGTESPAEVRALVK
                     DALRPPRGSRGFLNYWASTNAGIKVGTIVGRADEALEANPATAMTIYQIVLEEVAAAL
                     HHADDSSGVLSSNAAYAIDGLQKCAERLPPHGRADLFNYCLKTSAKDALQGWDYSWDL
                     LGLAADLVETAAGRKALFDRLSAMETELAPADPSDWSSEYKMSRIAEIKVVVIARLDG
                     DGAALEFLIAHKHFTQIRAILIEYHIVHGNLDAALEVIQEGQDELAQSGGYFYGIALT
                     YRRYLLEIAQDTGDTEAIIDHARALWLSRVGDVYYDMMKDAIPAAEWPAYREALLSDR
                     ACDAELAAWANAHEGLWDKVRDIVLAKPHLLSPYQLEIEKRFPDETADAYQEFARQLL
                     EGTALNRANYREAAGYLIRMRLLGHEEEGKAVARALIEQYPQRPAMIEELKRVL"
     gene            3540..4976
                     /locus_tag="PLC06_10565"
     CDS             3540..4976
                     /locus_tag="PLC06_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRO90930.1"
                     /translation="MSPDEFRANGKALIDWIADYYEQIEQYPVLSRVAPGEVRAQLPA
                     EPPTTGEPFAAMMADVERIIMPGITHWQSPNFYAYFPANASFPSILGELLSAGLGVQG
                     MIWQTGPAVTELETHVMDWMAEMLGLPEAFLSTSTGGGVIQDTASSAVLAALIAAREK
                     ITNYEVRSTNEEETAGASSLATGNDSRLRAYCSTQTHSSMEKAAGVAGVGRANVRAIA
                     VDERFAMRPEALRAAIESDLAAGLRPFFVVATVGSTSSLAMDPVRAIGEICREFGLWL
                     HIDAAMAGTAALCPEFRHYLDGVELADSYAFNPHKWMFTNFDCDCFWVADRAALIRSL
                     SILPEYLVNAATASGAVIDYRDWHVQLGRRFRALKLWFVIRHYGVEGLQALVREHVRL
                     AGLFAEWVAADPRFELAAPPVLNLVCFRLREGDEASRALLEKLNTSGRMYLTHTVLDG
                     KYALRLCVGQTHVEERHVREAWEEIRNS"
     gene            5005..5934
                     /locus_tag="PLC06_10570"
     CDS             5005..5934
                     /locus_tag="PLC06_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRO90931.1"
                     /translation="MTTTSAETSIRDLLYALENVGESPHPATVRAIYERREEAEAPML
                     ALFEAAYDDQWADPNDPRWYRFVHAGRFLMAWRTEAAIPIFARFYLDEAMQDTCEAFE
                     QDPGTFGPPAVPHFAAVVRAGTGRRWHYGRALSSGILARIGRDYPETREAVTAVLREQ
                     LPPLETIPQLAAADPATAWGREWDEMWAVFIGDLSALQDEASREQILAMFDADIVDNM
                     HLDRDYYLEAMNGKLDFHDSPYDLIAEYTNIHRHHVENQSRQERERRREPDPPVARPA
                     RADSTPKIGRNAPCPCGSGKKYKKCHGRMTNDE"
     gene            complement(5971..>6165)
                     /locus_tag="PLC06_10575"
     CDS             complement(5971..>6165)
                     /locus_tag="PLC06_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90932.1"
                     /translation="LRITNYELRIEEEEEENPQISRMEREKGGRGERELWVSGQWSVV
                     DGDRPLATDHCSLSSNLCHL"
BASE COUNT         1050 a         1967 c         1953 g         1195 t
ORIGIN      
        1 gctggtatgc ctatggcctc tacctgcccg acgacgtgct gcgtcgggtc tatcacgata
       61 acgcggccag ggtgttgggg ctttaacgac agacgaccga gcacggcaat agcgcgccca
      121 aggcacgaag tacgaaagag gcgcgcactc ttcattcgta attcgtaatt ccatggtata
      181 ctaccgttag atatatatct aatacgcgcg cccgcggcgc gttagtgaca cggagtattc
      241 atggaaaccc caacccaacg gccgctcgac cgttttcgtc gcggcctggc cacctacatg
      301 gccaacatcc gccttttctc gcgcaatgtg tggatggttc tcttctacgg catcggcatg
      361 ggcctcacct tcggcgtggt catgttcacc ttcaacttct acgtcctcag cctggggccg
      421 gcgttcgacg agggctttct gggcgtgctg caatcggccg cttccttcgc caccatcgcc
      481 atggccatcc ccgcggcgtg gatcgcccag cgctacccgc ccaagtgggt gatgatcgcc
      541 ggcggcctca tcggggcgat cgcctatatg ggcatcgtcc tctaccccac gcgcgagggg
      601 ctggtggtgt ttcgcatgct ggcgggcgtg tcgatgagcc tgggcatggt ggcgggcgcg
      661 ccgttcctca tggccaacac cggcgcggcc gagcgccaat gggccttcag cttccaggcg
      721 ggcctctcca ccgtggcgag cttcttcggc aacctgctgg ccggcatcct gcccggccgc
      781 ctgggagcgc tggtgggggt cgggccaacc gacacgctgg cctatcagct ggccctgggg
      841 ttgatgatcg ccctcagcct gttgtcgatc atcccgctgc tgctcatccg atcggcggcc
      901 gccccggcca aggccaacga gctgccctgg gtgcagctgc gccgccaccg gcggctgttc
      961 gccatcgtcc tgctgccctc gctgctcatc ggcctcggtg cgggcctcat gcagccgttc
     1021 atgaacgtct acttccgcaa cgtctacggc aagcccgacg cggccatcgg catcgtcttc
     1081 gccctgggcg ggctggccat ggccatcggc caattcgccg ggccgccgct ggccgaccgt
     1141 tacggcaaga ttcgcaccgt catgctgacc caggcgctgt cgatcccgtt tttggtcacg
     1201 ctcggtctgg gcgcgtggct ggtgacggcc ggattggcgc gggcggagct gttcttcttc
     1261 ctggccggca tcgcctacat cttccggctg gcgctcatga atctgagcaa ccccgtctac
     1321 cagaccttcg tgctggagcg cgtgccggag caggcccagg cgctggcggc cagtctgacg
     1381 gcgctggtct ttcaggtcgg ctggttcgtc atgccgcaga tcagcggcca gattcaggtg
     1441 acccatggcc ccggcggctt cgcctatatc ttcgccgggg tgacgatgtt ctacatcgtg
     1501 gccatcgcca cggagaagtg gttcttcggc ggcgaggaca aggcgcgggc ggcggggcgg
     1561 tgtgaattgc aagcgatgaa ctagcggcga gtgacgagcg acgagcgacg aaggcggctc
     1621 ctgtcgcccg acgagtgaag acgaagtacg aacgtcgtcg tcggtcgccc ggcgacggtc
     1681 gtttacagga ctcgcttgag ttcttcgatc atcgccggtc gttgcgggta ttgctcgatg
     1741 agggcgcggg cgaccgcttt cccttcttct tcgtgcccca gcaggcgcat gcggatgaga
     1801 tagccggccg cttcccgata attggcccgg tttagcgcgg ttccctccag caattgccgg
     1861 gcgaactcct gataggcgtc ggccgtttcg tcggggaatc gtttttcaat ttccagctga
     1921 tacggcgaca acagatgagg tttggccagg acaatatcgc gaaccttgtc ccacaggccc
     1981 tcatgggcat tggcccatgc cgcaagttcg gcgtcgcaag ccctgtcgct gaggagcgcc
     2041 tcgcgatagg cgggccactc ggcagcgggg atcgcatcct tcatcatgtc gtaatacacg
     2101 tcccccacgc gactgagcca cagcgcgcgc gcatggtcga tgatagcctc tgtgtcgcca
     2161 gtatcctgag cgatctccag caaatatctc cggtaggtca gagcgatccc gtaaaaatag
     2221 cccccggact gggccagctc atcctgccct tcctggatga cttccagggc ggcgtccagg
     2281 tttccatgaa caatatggta ttcaatcaat atcgcgcgga tctgggtgaa atgcttgtgg
     2341 gcgatcaaga actccagcgc ggccccatcg ccatccaacc gggcgatgac gaccaccttg
     2401 atctcggcga tacgggacat cttgtactcg ctcgaccagt cggacggatc ggccggcgcg
     2461 agttcggttt ccatcgccga cagccgatca aacagcgcct tgcgcccggc ggcggtttcc
     2521 acgaggtcgg cggccagccc aagcagatcc cagctgtaat cccagccctg cagggcatcc
     2581 ttggcggagg tcttcaggca atagttgaac aggtcggcgc ggccatgagg cgggagccgt
     2641 tcggcgcatt tctgcaaccc gtcgatggca tacgccgcgt tgctgctcaa cacgccggat
     2701 gaatcatcag catgatgcag ggcggcggcg acttcttcga ggacgatctg gtagatggtc
     2761 atggccgttg cgggattggc ctccagcgcc tcatcggcgc ggccgacgat ggttccgacc
     2821 ttgatgcccg cgttggtgga ggcccagtaa ttcagaaacc cgcggctgcc gcgcggcggc
     2881 cgcagcgcgt ctttcaccag cgcgcgcacc tcggccgggc tttcggttcc gcccaactgc
     2941 atcaggagcc aggactggaa ttcgcgatcg ccccgcgccg tttccatgac gatatcgacg
     3001 agctgctgtt gaggggccgc gtcgagcgcg gcgcgcagcc ggtcgagttt ggtggtgcgt
     3061 ttggtcgtcg ctttgcggcg cttgccctcg aactgttcgg ggaagacctc ctcgatggcg
     3121 tacagcatgg cggcgacgtg tttgcaatac gggccgtcgc tgtaggggca gttacaatag
     3181 acctcaagcg atttgtcgcc caggcgcttg atgcggactt cgtagggcgc tgtgccctgg
     3241 accacggccg cccagccgtc atcgccgtct tcctccaggt ccacgacctg gcccgctcgg
     3301 aagtattccc gtccacgggt gaggattgtc gccggaatct ctcgcttgaa attgttgagt
     3361 gtcaggggca tgggcttttc tccatacctg attttacccc acaacgaaca ggagcgacga
     3421 gcgacaaagg cgactcctgt cgcccgacga cagcctcatt cgtacttcgt cgttcgcact
     3481 tcgtacttca ggctttccaa ttcctgattc ctgattcata attcctgatt atgttacaca
     3541 tgtcccccga cgagttccgc gccaacggca aggcgctgat cgactggatt gccgactact
     3601 acgagcagat tgagcagtac ccggtgctga gccgggtcgc gccgggcgag gtgcgcgcgc
     3661 agctgccggc cgagccgccg acgacgggcg agccgttcgc ggcgatgatg gccgacgtgg
     3721 agcggatcat catgcccggc atcacccatt ggcagtcgcc caacttctac gcctacttcc
     3781 cggccaacgc ctcgttcccc tcgattttgg gcgagctgct gtcggccggg ctgggggtgc
     3841 aggggatgat ttggcagacc ggcccggccg tcaccgaact ggagacccac gtcatggact
     3901 ggatggcgga gatgctgggg ctgccggagg cgttcctttc gacaagcacc ggcggcggcg
     3961 tcatccagga cacggccagc agcgcggtgc tggcggcatt aatcgcagcg cgggagaaaa
     4021 ttacgaatta cgaggtacga agtacgaatg aagaggagac ggccggcgct tcttcgctcg
     4081 ccaccggcaa cgactcccgc ttgcgcgcct actgctccac gcagacccac tcatcgatgg
     4141 agaaggcggc gggggtggcg ggggtgggtc gggccaatgt gcgggcgatc gccgtcgatg
     4201 agcgcttcgc catgcggccg gaggcgctgc gggcggccat cgaaagcgac ctggccgccg
     4261 ggctgcggcc gttcttcgtc gtggccaccg tcggctccac ctcgtcgctg gcgatggatc
     4321 cggtgcgggc catcggcgag atctgccgcg agttcggcct gtggctccat atcgacgcgg
     4381 ccatggcggg cacggccgcg ctctgcccgg agttccgcca ctatctggac ggcgtcgagt
     4441 tggccgacag ctacgccttc aacccccaca agtggatgtt caccaacttc gattgcgact
     4501 gcttctgggt ggccgaccgc gcggcgctga ttcgctcgct gagcattttg ccggagtatc
     4561 tggtgaacgc ggccaccgcg tcgggggcgg tcatcgacta ccgcgactgg cacgtgcagc
     4621 tcggccgccg cttccgggcg ctgaagctgt ggttcgtcat tcgccattac ggcgtggaag
     4681 ggctgcaggc gctggtgcgc gagcatgtgc gcctggcggg gctgttcgcc gaatgggtgg
     4741 cggccgaccc gcgcttcgag ctggccgcgc cgccggtgct gaatctggtc tgcttccggc
     4801 tgcgcgaggg ggacgaggcc agccgggcgc tgctcgaaaa gttaaataca tccgggagga
     4861 tgtatttaac ccatacggtg ctggacggca agtacgccct gcggctgtgt gtgggccaga
     4921 cgcatgtgga ggagcggcat gtgcgggaag cgtgggagga aattcgtaat tcgtaattcg
     4981 taattcgtaa tttatactag cgacatgact acaacctcgg ccgaaacatc gatccgcgat
     5041 ctgctctatg cgctggagaa cgtcggggaa tcgccccatc ccgccaccgt gcgcgccatc
     5101 tatgagaggc gtgaagaggc cgaagcgccg atgctggccc ttttcgaggc cgcctacgac
     5161 gaccagtggg ccgaccccaa cgacccgcgc tggtatcgct tcgtccatgc cgggcgattc
     5221 ctgatggcct ggcgcaccga ggcggcgatt cccatcttcg cccggttcta cctggatgag
     5281 gccatgcagg acacgtgcga ggcatttgag caggatcccg gcacatttgg cccgccggcc
     5341 gtgccccatt tcgccgccgt cgtccgtgcc ggcacggggc ggcgctggca ctacggccgc
     5401 gcgctctcct ccggcatcct ggcgaggatc ggccgcgact atcctgaaac gcgcgaggcg
     5461 gtcaccgccg tgctgcgcga gcaattgccg ccgttggaga cgatcccgca attggccgcg
     5521 gccgacccgg ccacagcatg ggggcgcgaa tgggacgaga tgtgggccgt gttcatcggc
     5581 gacctgagcg cgctgcaaga tgaggcgagc agggagcaaa tcctggccat gttcgacgcc
     5641 gatatcgtcg acaatatgca tctcgatcgc gactattatc tggaggcgat gaacggcaag
     5701 ctcgatttcc acgactcccc gtatgacctg atagccgaat acaccaacat ccatcgccac
     5761 catgtcgaga accaaagtag acaggagcgg gaacggcggc gcgagcctga cccgcccgtc
     5821 gcccgcccgg cgcgcgccga ttccacgccc aaaatcggcc gcaacgcccc ctgcccctgc
     5881 ggcagcggca agaagtacaa gaagtgtcat gggcgaatga cgaatgacga atgacgaacg
     5941 acaagtgaag aagaagagaa tccacagatt tcacagatga cacagatttg aagagagcga
     6001 gcagtggtca gtggcgagtg ggcggtcgcc atcgaccacc gaccattgac cactgaccca
     6061 aagctccctt tctccccttc cccccttctc cctttccatc cgtgaaatct gcggattctc
     6121 ttcctcttcc tcttcaattc gtaattcgta attcgtaatt cgtaa
//
LOCUS       DAOMQG010000188         6149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5449_length_6149_cov_11.471776, whole genome shotgun sequence.
ACCESSION   DAOMQG010000188 DAOMQG010000000
VERSION     DAOMQG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_6149_cov_11.471776"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2241
                     /locus_tag="PLC06_10580"
     CDS             <1..2241
                     /locus_tag="PLC06_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRO90933.1"
                     /translation="YYNEENGYTVLRLRPDTRGLLPYAHGEPLATVVGNLPEVNPGEW
                     LKLTGQWLNHPKHGRQFQVELCEQSVPASVEGIKRYLGSGMVRGVGKVMAERIVNRFG
                     AETLDIIDEDPQRLGEVLGIGRKRVDNIIKAWEEQRAIKDVMVFLHGHGISTHLAVKI
                     YKQYGDQSLAVVQTTPYKLVQDVHGIGFKTADKIAQALGLSADDPARIEAGIHYTLSK
                     MAEDGHVYAPQDELEPEAADILQVSAERVSAVLESLENSELVRRETIVYPTADERRPA
                     AANGSAVREERAVYLPALYFSETGLTRQVRRLVEHPTSRLSGITNYELRITNKTFVDS
                     LTAQQRQAITATLSHKLTILTGGPGTGKTTTLRTLLDTLDATGHTYALASPTGRAAKR
                     LTEATGREAKTIHRMLEFKPGEGFGRNENNPVNADLIVIDEASMLDLMLAYNLLRAIG
                     SDSHLLLVGDIDQLPSVGAGDVLRDLIASDVAAVVRLETIFRQAANSLIIRNAHRINQ
                     GLMPETAGEQGGKGAGEKGAAEGHEPQSDFYLFIKEDPGETADLLVDIVAKRLPHKFG
                     LDPIDDIQVLAPMYNGLAGVTRLNELLQEALNPPGGRKVERRIGGRTFRVGDKVMQTV
                     NNYDKSVYNGDIGRITAIDVIEQTLSVAIDGAPVVYSFLEADELIHAFAISVHKSQGS
                     EYPCVVIPVVVQHYMMLQRNLLYTAVTRAKRLAILVGTRRAIQIAVRTNPTANRHTAL
                     DWRLKN"
     gene            2306..3022
                     /locus_tag="PLC06_10585"
     CDS             2306..3022
                     /locus_tag="PLC06_10585"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRO90934.1"
                     /translation="MEVQFAVSKVSKWASRESGDTLEMIERPQGGLSMVLVDGQTSGR
                     SAKAISNIVSRKAVQLLGEGVRDGAAARAAHDYLFTFRGGKVSATLNILSVDTRTRTI
                     VISRNNEAPVLVHTAANGFYELDDESKAVGVHRNTKPVITEVPIEVGTIVVAFTDGLR
                     HAGTLSGKGCSYDPYAALQEMVAAGQCDAASICDRLLDGAMAMDDGRPRDDISVIAIS
                     VRESREGDEVRRQSGRLTLS"
     gene            3139..3525
                     /locus_tag="PLC06_10590"
     CDS             3139..3525
                     /locus_tag="PLC06_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90935.1"
                     /translation="MPDDTLTPPLPLPPAPLRVAIVGPCAAGKSTLVKALREAGYEAR
                     HPAQEHSYVPDMWLRLVDPDVLIYLDVDYEVLLARRPNFGEREYLEREKARLAHARAH
                     ADLVVDTSGMTADEVATRVELFLEAK"
     gene            complement(3511..4368)
                     /locus_tag="PLC06_10595"
     CDS             complement(3511..4368)
                     /locus_tag="PLC06_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90936.1"
                     /translation="MKLAKQHPSICFLALLFCGLIGCTLAEHQEVYTTPGPAIALTST
                     TAIAQTDPTLVLEATSISRPSSRTPSPSGDDSLLYSPFQPEPTATPTVAPPAPPTPSP
                     TVTPMSTSTEVEGWLTYKNDFLGYRFSYPPQAIVDHHDVIELPENLTDEEYLAYLESN
                     FPIDLCAGVSYRTGFVTIYPSDDTLASYSTPCGITGIGDQRIEQWQEAITIGGESHMA
                     SAYRLYNRQTDQFEMEFYILIDVNNNLRIDFGAHKAGTPRSAQPRLSEQEFNEVLETL
                     RRIVESLRF"
     gene            complement(4430..5389)
                     /locus_tag="PLC06_10600"
     CDS             complement(4430..5389)
                     /locus_tag="PLC06_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRO90937.1"
                     /translation="MADKQWALYENNTHGFTLEYPADWDVTTLIEQQYPYAEPEKIIR
                     KYAFAGDEGYVTLSIFLSNEHNLSSWLKTQNEASPNLFPAASINGTVAGHPAVVFTVD
                     DNLIVFIEAGQYVFRFWHPASDNLALQLNLHMVRSFRLSGEEPASVITHIPDSIIREA
                     EDSLERAGLQTEQVNSCTSVQGAGCCDLPHLQTCYRFPCARENGQDKGNCTYLVCYKY
                     RGVPFSGNAGTWWGQVPSTPGWHSYTTPPNGASIAWWPTGEIYKVGHVGFIEYYSGSG
                     NPTVLEQGYCEYCTRQRPSEYSSPHGYIQKGLPPARIEIAPGW"
     gene            complement(5772..>6149)
                     /locus_tag="PLC06_10605"
     CDS             complement(5772..>6149)
                     /locus_tag="PLC06_10605"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRO90938.1"
                     /translation="CQGEMMINRLFVYGTLAPGRPNEHILRDLPGSWEPAIIRGWLVS
                     DGWGAAAGYPGIVLDQSGDEIQGFVFTSEHLAENWPMLDEFEGDDYERRLAKANLTDG
                     RAIDVFIYQLNRPGSTTGKKPGF"
BASE COUNT         1318 a         1701 c         1869 g         1261 t
ORIGIN      
        1 tattacaacg aggagaacgg ctacaccgtc ctgcggctgc ggccggacac gcgcggcttg
       61 ctgccctatg cccacggcga accgctggcc accgtcgtcg gcaacttgcc ggaggtcaat
      121 ccgggcgaat ggctgaagct gaccggccaa tggctcaacc accccaaaca tggccgccaa
      181 ttccaggtgg agctgtgcga gcagtcggtt ccggcgtcgg tcgagggaat caagcgctat
      241 cttggctccg gcatggtgcg cggtgtgggc aaggtgatgg ccgagcgcat cgtcaaccgg
      301 tttggcgcgg agacgctgga tatcatcgac gaggacccgc agcgcctggg cgaggtgctg
      361 ggcatcggtc gcaagcgcgt cgacaacatc atcaaggcct gggaggagca gcgggccatc
      421 aaggatgtga tggtgttcct gcatggccac ggtatctcca cccatctggc ggtcaagatc
      481 tacaagcaat acggcgatca gtcactggcg gtcgtccaga ccacgcctta taagctggtg
      541 caggatgtcc atggcatcgg cttcaagacg gccgacaaga tcgcccaggc attgggcctt
      601 tcggccgacg acccggcgcg catcgaagcc ggcatccatt acacgctgag caagatggct
      661 gaggacggcc acgtctatgc ccctcaggac gaactggaac cggaggcggc cgacatcctg
      721 caggtttcgg ccgagcgggt gagcgccgtg ctggagtcgc tggagaacag cgagctggtg
      781 cggcgggaga cgatcgtgta tccgacggcc gacgaacggc gaccggccgc cgccaacggt
      841 tctgccgtcc gcgaagagcg ggcggtctat ctgccggcgc tctatttctc ggagacgggg
      901 ctaacccgcc aagtgcggcg tctggttgag cacccgacaa gccggctcag cggaattacg
      961 aattacgaat tacgaattac gaacaagacc ttcgtcgatt ccctgaccgc ccaacagcga
     1021 caagccatca ccgccacgct gtcccacaag ctgaccatcc tgaccggcgg gccggggacg
     1081 ggcaagacga cgacgctgcg gacgctgctc gatacgctcg acgcgaccgg ccacacttac
     1141 gcgctggcct cgcccaccgg ccgggcggcc aaacgcttga ccgaggccac cggccgcgaa
     1201 gcaaagacca tccatcgaat gctggaattc aagccgggcg agggcttcgg ccgcaacgaa
     1261 aacaacccgg tcaatgcgga tctgatcgtc atcgacgagg catcgatgct cgacctgatg
     1321 ctggcctaca acctgttgcg ggccatcggc tccgatagcc acctgctgct tgtgggcgat
     1381 atcgaccagt tgccgtcggt gggggcgggc gacgtgctgc gcgacctgat cgcctcggat
     1441 gtggccgccg tcgtgcggct ggagacgatc ttccggcagg cggccaattc gctgatcatt
     1501 cgcaacgctc atcggattaa tcaggggttg atgccggaga cggcggggga gcagggggga
     1561 aagggagcag gagagaaagg ggcggccgaa ggtcatgaac cccaaagtga tttctacctg
     1621 ttcatcaagg aagacccggg cgagacggcc gatttgctgg tcgatatcgt cgccaagcgg
     1681 ctgccgcata agttcgggct ggaccccatc gacgatattc aggtgctggc gccgatgtac
     1741 aacggcctgg ccggggtgac gcggctgaat gagctattgc aggaagcgct caacccgccg
     1801 ggcggccgca aggtggagcg tcgcatcggc ggtcgcactt tccgcgtcgg cgacaaggtg
     1861 atgcaaacag tcaacaacta tgacaaaagc gtttataatg gtgatattgg acgtatcacc
     1921 gccatcgacg tgatcgaaca gacgttgtcg gtcgccatcg acggcgcgcc ggtggtttac
     1981 agctttctgg aggcggacga actgatccat gctttcgcca tcagcgtcca caagtcgcag
     2041 gggtcggaat atccgtgcgt ggtcattccg gtggtcgtgc agcattacat gatgctgcag
     2101 cgcaatttgc tctacacggc cgtgacgcgg gcaaagaggc tggcgattct ggtcggcacg
     2161 cgccgcgcca tccagatcgc cgttcgcacc aacccgacgg ccaaccgtca cacggcgctg
     2221 gactggcggc tgaaaaatta ggaattacga atcaggaatg aagcgcgctt ctcattcgta
     2281 attcgtaatt cgtaattctc ttgctatgga agttcaattc gcagtctcaa aggtatcgaa
     2341 atgggcgtcg cgggagagcg gcgacacgct ggagatgatc gagcggccgc agggtggttt
     2401 gtcgatggtg ctggtcgatg ggcagacgag cggccgcagc gccaaggcga tcagcaacat
     2461 cgtctcccgg aaagcggtgc aactgctggg cgagggagtg cgcgacggcg cggcggcccg
     2521 tgccgcccac gactatctgt tcacctttcg cggcggcaag gtgtcggcca cgctcaatat
     2581 cctctccgtg gacacccgca cgcgcacgat cgtcattagc cgcaacaacg aagcgccggt
     2641 gctggtgcat accgcggcca acggcttcta tgagctggat gatgagtcca aggcggtggg
     2701 cgttcatcgc aataccaagc cggtgatcac cgaagtgccg atcgaggtcg gcaccatcgt
     2761 cgtggccttc accgatggcc tgcgccacgc ggggactttg agcggcaagg ggtgcagcta
     2821 tgatccgtat gccgccttgc aggagatggt ggcggccggg caatgtgacg cggcgagcat
     2881 ctgcgaccgc ctgttggatg gggcgatggc catggacgac ggccgccccc gcgacgacat
     2941 cagcgtcatt gccatctccg tgcgggagag ccgggaagga gacgaagtgc ggcggcagag
     3001 cgggcggctg acgttgtcct agatacgaga tacgagatac gaaatacgag ttcagacgac
     3061 ccctccaccg gccgctgatc tactgaccta ctgacctact gacccactga cctactgacc
     3121 cactgaccaa cttttcccat gccggacgac accctcacac ccccactccc cctcccccct
     3181 gctcccctgc gcgttgccat cgtcggccct tgcgcggcgg gcaagagcac gctggtgaag
     3241 gcgctgcggg aggcgggcta tgaggcgcgt catccggcgc aggaacattc ctatgtgccc
     3301 gatatgtggc tgcggctggt cgatcctgat gtgctgatct atctcgatgt cgactatgag
     3361 gtgctattgg cgcggcggcc gaatttcggc gagcgcgaat atctggagcg ggagaaggcg
     3421 cggctggccc acgcgcgggc gcacgcggat ttggtggtgg atacgagtgg gatgacggcc
     3481 gatgaggtag caaccagagt ggagcttttt ctagaagcga agtgattcga caattcgccg
     3541 cagtgtttca agtacttcgt tgaattcctg ctcgctcaac ctcggctgcg cacttctggg
     3601 tgtacccgcc ttgtgggcac cgaaatcaat gcgcaggtta ttgttgacgt cgattagtat
     3661 ataaaattcc atctcgaatt ggtcggtctg ccggttatac aatcgataag cgctggccat
     3721 gtgcgattcg ccgccgattg tgatggcttc ttgccattgc tcgatgcgtt gatcgccgat
     3781 gccggtgata ccacacggcg tggagtagga agcaagcgta tcgtccgagg ggtatatggt
     3841 tacaaatccc gtcctgtagc tcacacctgc acaaagatca atgggaaagt ttgattccag
     3901 ataggctaga tactcctcat cagtcaagtt ctccggcagc tctatgacgt cgtggtgatc
     3961 gactattgcc tgcggtggat agctgaaacg atagccgagg aagtcatttt tgtaggtgag
     4021 ccagccttcc acttcggttg acgtggacat gggcgtaaca gttggcgagg gtgttggggg
     4081 cgctggcggt gcgacggtgg gcgtcgctgt tggctcaggc tgaaatgggc tatatagtag
     4141 gctgtcgtcg cccgacggtg agggtgtgcg cgatgatggc ctactgatcg acgtagcttc
     4201 caggaccagt gtcgggtcag tttgggcgat ggcggtggtt gaggtgagtg cgatggccgg
     4261 acctggggtt gtgtaaacct cttggtgctc tgcgagcgtg cagccgatca ggccgcaaaa
     4321 cagaagcgcc aagaagcaga tagaagggtg ttgttttgcc agtttcatcg tatgaaaatc
     4381 cccgcaagca agagcctgtt cataggactg tggttagaaa tagcaacagt taccaccctg
     4441 gggcgatttc tattcgcgct ggtggaagtc ctttttggat atagccatga ggcgatgaat
     4501 attcgctagg acgttgtcgt gtgcaatatt cgcagtagcc ttgctcaagc actgtggggt
     4561 tcccactgcc cgaatagtat tcaatgaagc ccacatgacc aactttgtag atttcaccgg
     4621 ttggccacca agcgatgctc gcaccattgg gtggtgtagt gtacgagtgc catccgggtg
     4681 tacttggaac ctgtccccac caagtacctg cattaccgct aaacggtacg ccacgatact
     4741 tataacacac gagataggta cagttgccct tgtcttgtcc gttctctcgt gcgcacggaa
     4801 atctatagca agtctgcaaa tgaggaagat cgcaacaccc ggcaccctga acggaggtgc
     4861 agctgttgac ttgctcagtt tgcagcccag ctctctctaa agaatcttct gcctccctga
     4921 ttattgagtc aggtatgtgg gtgattacac ttgcaggttc ttccccagaa aggcgaaaag
     4981 aacgcaccat atgcaagtta agttgcagtg caaggttatc cgatgctgga tgccaaaacc
     5041 gaaacacata ctggccggct tctatgaaga cgattaggtt atcgtctacg gtgaaaacaa
     5101 ctgccggatg accggctacc gtaccgttaa tacttgccgc tggaaacaaa ttcggacttg
     5161 cctcattctg ggtttttagc caagaggaca agttatgctc atttgacagg aagatcgaaa
     5221 gcgtgacata cccttcgtcc ccagcaaagg catactttct gatgatcttt tctggctccg
     5281 cataggggta ttgctgctcg ataagtgttg ttacgtccca atctgctggg tactccaatg
     5341 taaatccgtg ggtattgttt tcatacaatg cccactgctt atcagccatc aatctcgacg
     5401 caagttgtta ctccttgtca atgggaggcc tgggggttgt tgtgcccggt gcggcagcaa
     5461 ccaacaatag gataagcatc caagcccaca agaaatcaac ctttctgttt gccatgactt
     5521 atgcctcctt gacaactcaa aggtgatgta ctcacttaac aacgtaacat gccgcttcct
     5581 tatagatcaa gtaacgaatg tcacgcagga ggcagttgat tgtcatatag gagtattggg
     5641 gtggtaatgt gggtagccaa gcagccagca agtgaatgtc aagggtgcca tggacgaatc
     5701 aggcggcgaa catcgttcgc cgccgacaaa caaaaagccc ctctcgcaag aggggtttat
     5761 cgggtcgtcg attagaaacc gggtttcttc ccggtagttg acccgggccg attcagttga
     5821 tagatgaaga catcaatggc gcggccgtcg gtgaggttag ccttagccag tctgcgctcg
     5881 taatcgtcgc cctcaaactc atcaagcatg ggccaattct ccgccaggtg ttccgaagtg
     5941 aaaacaaagc cttgaatttc atctccagat tgatccagca cgatgccggg atagcctgcc
     6001 gccgcgcccc agccgtcgga taccagccat cccctgataa tggcaggctc ccaggagcca
     6061 gggagatcgc ggagtatatg ctcgttgggc cggccgggcg caagcgtacc atagacaaac
     6121 agccgattaa tcatcatctc tccttggca
//
LOCUS       DAOMQG010000189         6087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5513_length_6087_cov_8.589191, whole genome shotgun sequence.
ACCESSION   DAOMQG010000189 DAOMQG010000000
VERSION     DAOMQG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5513_length_6087_cov_8.589191"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLC06_10610"
     CDS             complement(<1..454)
                     /locus_tag="PLC06_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HRO90939.1"
                     /translation="MRVLMLSKALLVGTYQTKLEAIARIPGVELLAVVPPGWDDPSGP
                     VAFERRFTAGYELLVEPIRFNGRYHIYYFPDLPRHLAQFRPDIVHIDEEPYNLATWLA
                     MRQARKVGAKTLFFTWQNLHRRYPLPFSQIERQVLDGVDYAIMGNQAAV"
     gene            633..1562
                     /locus_tag="PLC06_10615"
     CDS             633..1562
                     /locus_tag="PLC06_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRO90940.1"
                     /translation="MMQADSPAIPLADLRLGIVGLGLMGGSLALALRGRVADIVAVER
                     QPDTRQLALREGVVSVAVDDLSTALPLVDLLVLAAPVRVILDTLGRLPSLLPAECAVI
                     DLGSTKRAVVAAMDALPPSFAAIGGHPMCGKETSGLASAAADLYRGQTFFLCRSRRTT
                     LALEATVLALVAALGARPVFMGAAAHDAAVAMVSHLPALLSAALMRAAADEALWPVGA
                     SGFRDTTRLAGSDPRMMLDILLTNREAVLAALAGYQAELAAVGRALELEDEAALSEWL
                     AAAQVTYAAWRRFKSSEYLLSNREGDEETGKIA"
     gene            1617..3035
                     /locus_tag="PLC06_10620"
     CDS             1617..3035
                     /locus_tag="PLC06_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRO90941.1"
                     /translation="MSNQSRDPRRTTGVKLPAWALLLILAAGLLLLTLSSLWLFRTIR
                     GLASIPDSAGEFNPTTGQLSPGQTAPQVQMGTSVASGGQIAPISLPTWSGTERVNILL
                     LGVDLRCEEEGPTHSDTIIVATIDPVSQSAALLSLPRDLWVEIPNFGVNRINQAYFLG
                     QAYELPGGGAALAADTVEAFLGITIDYYAAVDFKAFVDFVDLMGGVVVDVPERIEDPH
                     YPDNCYGYDPFTIEAGKQRLDGATALKYARTRITFGGDVDRAGRQQQVIMAVREQATQ
                     LNTLPQLILNAPQLWQSFQDNVDTDLELNEALQLANLVRNIPDGSIRNVVLDYSYVYN
                     DTTFDGQQVLVPLREEVRALRDELFAPPVVPTPVIEALPTVITVEEARVAVFNGTPTF
                     GLASDTQAYLLRQNVNVTVVGNADSATYTTTQIIDYGSHPGTVQYLVQLLNVPPLNVS
                     TGTNPAGEFDILVILGEDWQVP"
     gene            3020..3808
                     /locus_tag="PLC06_10625"
     CDS             3020..3808
                     /locus_tag="PLC06_10625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO90942.1"
                     /translation="MASSLIRLTAIILTRNEARHVVECIDSLRWADRVIVFDSLSDDD
                     TVALARAAGAEIFQRAFDNYAAQRNAALEAAAGSGEGEQWVFFVDADERGTPELGEEI
                     RRVIETRPENGWNVPRHNYIFGRLTLGAGWFPDYQFRLMRLGHARYTRPVHELGEVDG
                     AVGDLQNPLIHHNYRDPAHFHAKQRFYVEHDAGILRDQGIRPKPRNYVLQPLRQFWWR
                     FVTLKGYRDGLHGLRLSLYMAWYEWRKYRRLGDLWREGRANNAD"
     gene            3798..4025
                     /locus_tag="PLC06_10630"
     CDS             3798..4025
                     /locus_tag="PLC06_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90943.1"
                     /translation="MPIEVDCTFDESGRVRVRRVRLGRPWQVVEQGRQWADGDGRHVL
                     VMLPDGARELLLRADTLVWELRELPGGRGMV"
     gene            complement(4046..4324)
                     /locus_tag="PLC06_10635"
     CDS             complement(4046..4324)
                     /locus_tag="PLC06_10635"
                     /inference="COORDINATES: protein motif:HMM:NF023857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HRO90944.1"
                     /translation="MNDDKAKIPDMTSHEEIADFWDTHSIADFWDETEPTEFELDANA
                     RRRYLVGLDRDLLARARRLALERGLSTESMVNLLLEQRLNQLETTASG"
     gene            complement(5127..5585)
                     /locus_tag="PLC06_10640"
     CDS             complement(5127..5585)
                     /locus_tag="PLC06_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90945.1"
                     /translation="MLTKDGILIIGNDDTIKPGLLEVDFLASSLGAMTRFNGANDGYS
                     RYSGSGPLEGGLICYFSLYFYDGILDMVIWTPKWPGTGNSWAEWSEEEELRVKELNDR
                     LLEQYLGPPPYSYAWGSIESDYDMKGGSSGIVMKMLGKEGINREASLPIE"
     gene            complement(5630..5953)
                     /locus_tag="PLC06_10645"
     CDS             complement(5630..5953)
                     /locus_tag="PLC06_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90946.1"
                     /translation="MEVDKLLDTLKSIEDLLRKSNHDDKAEWLADKGATINDGSRDKM
                     LDALESIRGHMVGMGSLTDLYLRPIPETGMTPEEARGVLLGLVESLYNEIVSILAGIG
                     LEDDG"
BASE COUNT         1138 a         1867 c         1848 g         1234 t
ORIGIN      
        1 cgacggccgc ctggttgccc atgatggcgt aatcgacccc gtccagcacc tgccgttcaa
       61 tttggctgaa cggcagcggg tagcggcgat gcaggttttg ccaggtaaag aacagcgttt
      121 tggccccgac cttgcgagcc tgtcgcatgg ccagccacgt cgccaggttg tacggctctt
      181 cgtcgatgtg gacgatgtcg ggcctaaatt gggccaggtg gcgaggcaaa tcgggaaagt
      241 aatagatgtg gtagcggccg ttgaagcgaa tcggctcgac gagcagctca taccccgcgg
      301 tgaagcggcg ctcgaaggcc accgggccgc tggggtcgtc ccaacccggc ggcacgacgg
      361 caagcagttc cacgccgggg atgcgggcga tggcttccag cttggtctga taggtgccca
      421 ccagcagggc cttggagagc atgagtacgc gcatgagtta ttggattcgt cgcaaagtgg
      481 gccaatagcc gatcaactat agccgcttcc tgttggatga caatcgcggc ggttgtagct
      541 caacctgcca ggttgagcct tctcgcaagc cggcagcttg cgccacaggc ggagaatatg
      601 cgattggctc gcccttcgtt ataatgtcgg gcatgatgca agccgacagc cccgccatcc
      661 ccctggccga ccttcgcctg ggcattgtcg ggctggggct aatgggcggg tcgctggcgc
      721 tggcgctgcg cggccgggtc gccgacattg tcgccgtcga gcggcaaccc gacacgcgcc
      781 agctggcgct gcgcgagggc gtcgtcagcg tcgccgtgga cgatctgtca actgcccttc
      841 ccctcgtcga tctgctggtg ctggccgcgc cggtgcgggt tatcctggac acgctcggcc
      901 gcctgccgtc cctgctgccg gcggagtgcg ccgtgatcga cctgggcagc accaagcgcg
      961 cagtcgtggc cgccatggac gcgctgccgc cgtcgttcgc cgccatcggc ggccatccga
     1021 tgtgtggcaa ggagacgtcg gggctggctt cggccgcggc cgacctgtat cgcggccaga
     1081 ccttcttcct gtgccgctcg cggcgcacca cgctcgcgct tgaggcgaca gtcctggcgc
     1141 tggtggcggc cctcggtgcg cgcccggtct tcatgggcgc ggccgcccac gatgcggccg
     1201 tggccatggt cagccatctg cccgcgctcc tgtcggcagc gctgatgcgc gcggcggccg
     1261 atgaagcgct gtggccggtg ggcgcttcag gcttccgcga caccacgcgc ctggcgggca
     1321 gcgacccgcg catgatgctc gatatcctgc tgaccaaccg cgaggcggtg ctggcggcgc
     1381 tggcgggcta tcaggcggag ctggccgccg ttggtcgggc gctggagctg gaggacgagg
     1441 cggcgctgag cgagtggctg gccgccgccc aggtgaccta tgccgcctgg cggcgcttca
     1501 aatcgtctga gtatttgcta tcaaaccgag agggcgacga ggaaacgggt aaaattgcct
     1561 gatcatcggc ccgtggctat aatgccgcgt gcccgtacct gatagaatct tgtcttatgt
     1621 ctaatcaatc ccgtgatcct cgccgcacta ccggcgtgaa actaccggca tgggccttat
     1681 tattgatcct ggcggcgggg ctgctgctgc tgaccctcag ctcgctgtgg ctgttccgca
     1741 ccattcgcgg gttggcctcg attcccgaca gcgccggcga gtttaacccc acgaccggcc
     1801 agctttctcc aggacagacc gcgccgcagg tgcagatggg cacctccgtg gcctccggcg
     1861 ggcaaatcgc acccatctcg ctgccgacct ggtcgggcac ggagcgggtg aatatcctgc
     1921 tgctcggcgt cgatttgcgc tgcgaggaag aggggccgac ccacagcgac acgatcatcg
     1981 tcgccaccat cgacccggtg agccagtcgg cggcgctgct gtcgttgccg cgcgatctgt
     2041 gggtggagat tcccaatttc ggcgtcaatc gcatcaatca ggcctacttc ctgggccagg
     2101 cctatgagct gccgggcggc ggcgcggcgc tggcggccga cacggttgag gcgtttttgg
     2161 gcatcacgat cgactattac gcggccgtcg atttcaaggc gttcgtcgat tttgtcgatc
     2221 tgatgggcgg ggtcgtggtc gatgtgcccg aacgcatcga agacccccac taccccgata
     2281 attgctacgg ctacgatccg ttcaccatcg aggccgggaa acagcggctg gacggcgcga
     2341 cggcgttgaa atacgcgcgc acgcgcatca ccttcggcgg cgacgtggat cgcgccggcc
     2401 gccaacagca ggtcatcatg gccgtgcgcg agcaggcgac ccagctgaac acgctgccgc
     2461 aactgattct caacgcgccg cagctgtggc aatcgttcca ggacaatgtc gataccgatc
     2521 tggagctgaa cgaggcgctg cagctggcca atctggtcag gaatatcccc gacggcagca
     2581 tccgcaacgt cgtgctcgat tactcctatg tgtataacga cacgaccttc gatggccagc
     2641 aggtgctggt gccgctgcgc gaggaagtcc gcgcgctgcg cgacgagctg ttcgcgccgc
     2701 cggtcgtgcc cacgccggtc atcgaggcgc tgccgacggt gatcaccgtc gaggaagcgc
     2761 gggtggccgt cttcaacggt acgccgacct tcggtctggc gtccgacacc caggcgtatc
     2821 tcttgcggca gaatgtgaat gtcacggtag tcggcaacgc cgattcggcc acctacacca
     2881 cgacccaaat catcgattac ggctcccacc ccggcaccgt gcaatatctt gtccagttgt
     2941 tgaacgtccc gccgctgaat gtgagcacgg gcaccaaccc ggcgggcgag ttcgacatcc
     3001 tcgtgatcct gggcgaagat tggcaagttc cctgatccgg ctgaccgcca tcatcctcac
     3061 ccgcaatgag gcccgccatg tagtcgagtg catcgacagt ttgcgctggg ccgaccgcgt
     3121 tatcgtgttc gactcactga gcgacgacga cacggtcgcg ctggcccgcg ccgccggagc
     3181 cgagattttc cagcgggcgt tcgataacta cgccgcccag cgcaacgcgg cgcttgaggc
     3241 ggccgccggt tcgggcgagg gcgagcagtg ggtgttcttc gtcgacgccg acgagcgcgg
     3301 cacgccggag ttgggcgagg agattcgccg cgtcatcgag acccggccgg agaacggctg
     3361 gaacgtgccg cgccacaact atatcttcgg ccgcctgacg ctgggggcgg gctggtttcc
     3421 cgactaccag ttccggctga tgcgcctggg ccatgcccgc tacacgcgcc ccgtccacga
     3481 gctgggcgag gtggacggcg cggtcggcga tctgcaaaac ccgctgatcc accacaacta
     3541 ccgcgacccg gcccatttcc acgccaagca gcgtttctac gtggagcacg acgcgggcat
     3601 cctgcgcgat cagggcatac gacccaagcc gcgcaactac gtgctgcaac cgctgcgcca
     3661 gttttggtgg cgattcgtca cgctgaaggg gtatcgcgac gggctgcacg ggctgcggct
     3721 gagcctctac atggcctggt atgagtggcg caagtatcgc cggctgggcg acctatggcg
     3781 cgaagggaga gcgaacaatg ccgattgaag tcgattgtac gtttgacgaa agcggccgcg
     3841 tgcgggtgcg gcgggtgcgg ctgggacggc cgtggcaggt cgtggagcag ggccggcaat
     3901 gggccgacgg cgacggccgg catgtgctgg tgatgctgcc tgacggcgcg cgcgagctgc
     3961 tcctgcgggc cgacacgctg gtgtgggagc tgcgggagtt gccgggcggg cgggggatgg
     4021 tgtagggtgg ggtggggtga gcggtctaac cggacgctgt cgtttccaac tgattcagcc
     4081 gctgctccaa cagcagattc accatgctct ccgtggacaa tcctcgctcc agcgctaatc
     4141 gccgggcacg agccaacaag tcgcgatcaa gaccaactaa ataacgacgg cgagcgttgg
     4201 catccaactc aaattcggtc ggttccgttt catcccaaaa atcggctatg ctgtgggtgt
     4261 cccagaaatc ggctatttcc tcatgggaag tcatgtcggg gatttttgct ttatcgtcgt
     4321 tcataggcct ttcgctcgct gctgtccata tctcgcgctg ttagaacgag agcctcctgt
     4381 gactgttcac gggtcatgga gtatgtcgat atccaaactt cgtagggctg acccaagtcg
     4441 ttcaacattt tagacaattg atagcgaact acttccccca tctctagttc gatcttctga
     4501 acaacgtcat cttgaacaac gtaccggtgg tggagccgta ccggatatat atcgcgcggc
     4561 acggggatca agatgtcata ggcgattcgg gatgcgaggg atggggagtt gcctcctaaa
     4621 ggctagggag ggaagatttc catcgccaac ggggaaagga tagtcaaggc ttcttcccag
     4681 gtcgtttttc ccggctcgat accccaccag cagggcaacg cgcatcctcc attctcatca
     4741 aacaggtggc gtatctcatc cagtatttgc tgtggggtga gggtcgcggt tgctgcgatt
     4801 gtgtgggtag gcagatcggc ggtaggcggg gataccgtgc gggtcggctt gggtgaagaa
     4861 atggtggtcg gtgtcgcagt tgccgatgat ggataggcag acctctctgc cgcgtctccc
     4921 cgtaatcccg ttcgggcact cggcgacatg cacgaagcca aagccagtat gcccatcaag
     4981 aacattatta caccgattgg tccattgcga cgcctgatat acataatgtg atgaatgcca
     5041 aaagccacgc cacgcgctta atcttttcaa gagcctattg agtctatttt gaactctttc
     5101 aaaaccaagt gaaggaactc gctgcatcat tcaatcggaa ggctcgcctc ccgattgata
     5161 ccctcttttc ctaacatctt cataacgata ccactggaac cacccttcat atcatagtct
     5221 gactcaattg acccccacgc atagctgtaa ggtggcgggc cgagatattg ttcaagcaat
     5281 ctatcgttca attctttgac ccttagttcc tcctcttcac tccactcggc ccaactattc
     5341 cccgttcccg gccactttgg cgtccatatg accatatcca ggataccatc gtagaagtag
     5401 agagagaaat agcaaatcaa gccgccttca agggggccgc ttccactata acgactatag
     5461 ccgtcattcg caccgttaaa gcgcgtcata gcgcctagtg aagaagccag aaaatccacc
     5521 tcaagcagcc ccggtttgat tgtgtcatca tttccaatga tcagtatgcc gtctttggta
     5581 agcattgcgg gactccacac attagccttc tttttattgg catccgaatt tacccgtcat
     5641 cctcaaggcc gatgccggca agaatagaaa cgatctcatt ataaaggctc tctactaaac
     5701 caagcaggac tcctctagcc tcctcgggag tcatgcctgt ttctggaatc ggccgcaagt
     5761 acagatctgt taaagacccc atcccgacca tatggccgcg aatactttcc agagcatcaa
     5821 gcatcttatc tcttgaaccg tcgtttatag tcgctccctt atcagctaac cattcggcct
     5881 tgtcatcatg attgcttttc cgcagcaggt cttctatcga cttcaaggtg tccagtagtt
     5941 tatcaacctc catacgggag gtcatattgg aaatatttac tttaattagc tttcgtaagc
     6001 ttttcaccta tcccggcgcc agccccggca aaacccgcca caacgcatac accgccatcc
     6061 cccccagcac ggccgcgaac cccagcg
//
LOCUS       DAOMQG010000190         6075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5523_length_6075_cov_5.901993, whole genome shotgun sequence.
ACCESSION   DAOMQG010000190 DAOMQG010000000
VERSION     DAOMQG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_6075_cov_5.901993"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="maeA"
                     /locus_tag="PLC06_10650"
     CDS             complement(<1..1017)
                     /gene="maeA"
                     /locus_tag="PLC06_10650"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HRO90947.1"
                     /translation="MSSENTKAADAAAELPEDFPRGTQLLFNPIFNRGTGFSKAEREA
                     FGLRGLLPPRVLSLHHHAARILENFYKKPTDLEKYVYMIGLEDRNETLFYRILIDNLD
                     TMMPIVYTPTVGQACQQYGHIFRRPRGLFISAKDKGDIAELLKNWPIDDIRVIVVTDG
                     ERILGLGDQGANGMGIPVGKLSLYTACAGIPPTATLPIMLDVGTENQTFLNDPLYIGL
                     PERRLQGQAYDDFLEEFMQAAVERFPNAVIQFEDFANHHAFELLERYRDRYCVFNDDI
                     QGTAAVTLAGLFAALRLIAAEGGPRRKLSEQRLLFLGAGEAGVGIADLVVTAMVDEGT
                     SVDEG"
     gene            1286..2212
                     /locus_tag="PLC06_10655"
     CDS             1286..2212
                     /locus_tag="PLC06_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO90948.1"
                     /translation="MVLDRIAQIALIFFGALVLVLLVLGLAVSCRETGAGPIGSATVV
                     LDSATADPFATATTNAGAATATTDPFAPTADPFATATTDAGAATATTDPFAPTADPFA
                     TATTDAGAATATTDPFAPTVDPLATPTLAPAATVTIDPAAPIATATADPAATAAPGAT
                     ATPEATTISATPASGATPASGATPASGATTVAPPPSGMVPGATARHAVSRGEWVLQIA
                     RCYGVSYGSVLNANYLANPDYILPGWILTIPDIGRQGRIVGPPCVVDYTVVAGDTWDS
                     LAARYQTTTAILMKANPGALVAGRSIWVPRVP"
     gene            2248..3180
                     /locus_tag="PLC06_10660"
     CDS             2248..3180
                     /locus_tag="PLC06_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90949.1"
                     /translation="MAKTLSRAAFDRAAQFMREEARPLERALFAYHFEKGSRTRVLAA
                     LVPYQNPDGGFGHGIEPDIRSGASSVIGTVTALDILRRVRADEDTPGLPAALAYLVDA
                     YDVESERWPIVPPDIENAPHAPWWSYADSTENFRGFWANPRAAVVGHLQQYLRLSPSP
                     FTEIARHAVVDDLMRYSQRMEMHDLLSFLDLLETDGLPREHYESILDKLRRALPYSVV
                     TDPAKWGGYGLRPLEVVYFPGSPLAGVLDQAVIDANLDYLIDEQEPNGSWAPNWTWDF
                     IDPFAWDAAEREWRGVLTLRKLRTLRAYGRIEGL"
     gene            3202..4032
                     /locus_tag="PLC06_10665"
     CDS             3202..4032
                     /locus_tag="PLC06_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HRO90950.1"
                     /translation="MTGFGEWDAVEQAEGGPPATIQSLRADFEALGLRPGQVVLVHSS
                     LKSLGWVSGGPVAVILALEMTLGAAGTLVMPTHSADLSDPARWRNPPVPEAWWPIIRD
                     TMPAYDPDLTPTRGMGAIPEAFRRGRGVARSNHPQVSFAAKGPHAAQITGGHPYDYGL
                     GEGSPLARLYELDGWVLLLGVGHGNNTSLHLAENRANFPGKQVVENGAPVRVDGRREW
                     VTLHDIDFDDEDFSAIGAALERETGAVHVGPAGRGVARFMRQRALVDFAVAWMEQHRR
                     "
     gene            complement(4029..5018)
                     /locus_tag="PLC06_10670"
     CDS             complement(4029..5018)
                     /locus_tag="PLC06_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRO90951.1"
                     /translation="MRRRLILLILIPLLVILGLTALVVGVLRAANISDVTLLESVIGS
                     SPTAQALVTYFPLVRFPLQLVFVVVVFGLAWLVSRVSDRLAGRLLNMAGYDTGRPAAD
                     DPPAMNNDLATLVIRRRETVLQLASGFISFIAFTVALLMAAGQFFSLTNMALVVTVAI
                     NAFGFAARDYIGDMLNGISNIFENRFNIGDNVQIFRVGDKLEGTIEHITVRTLSIRTR
                     TGELINVPQGEVRTLLNFTRGSYSGVDVIVKVAAADLPEAMAELLMLAQDAPDLLPDC
                     IEPWSVIARDGTLGSAADIRIHAKARYGYGAGLRLRIMMLAEERLAAAGISPA"
     gene            complement(5015..6040)
                     /locus_tag="PLC06_10675"
     CDS             complement(5015..6040)
                     /locus_tag="PLC06_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRO90952.1"
                     /translation="MNARRILFWLLSPLWLALAGISLMVGLAFVSGNNPAGMLVAELF
                     DGTPVELQLATLTVRLLLLPFQFLFIGLAMAAALWVFRRRDRLTEWVLGEPLAERLTA
                     VEGVADELPAVLQPTRRRTVQQILSSIIAFTAIVAGTLLAAAQFIARDDLALIVAALT
                     SGLTWGARLPIGDLLGGITNIFENNLAIGDRIYYKLFEKSVEGTVESVDLRFLSVRAR
                     SGELTSIPFGDLRIFRNYSRGNFIGVYASFPIAAKDLRRAVALLTEMAPESPDLVPQL
                     MEPWQPMGLEGVLGATVDVFLFGKTDQKWEDEAQMAIHAVVHDRFRAAGIPLQGKGSQ
                     DKDDNPL"
BASE COUNT         1047 a         2022 c         1997 g         1009 t
ORIGIN      
        1 tccctcgtcg accgacgttc cctcgtcgac catggccgtc acgaccagat cggcgatgcc
       61 gacgcccgcc tcgcccgcgc ccaggaacag caaacgctgc tcgctcagct tgcggcgtgg
      121 cccgccctcg gcggcgatca ggcgcagcgc ggcgaacaac ccggcgaggg tgacggccgc
      181 cgtgccctgg atgtcgtcgt tgaagacgca atagcggtca cgatagcgct ccagcagctc
      241 gaaggcgtgg tgattggcga aatcctcgaa ctggatcacc gcgttgggga agcgctcgac
      301 ggcggcctgc atgaactcct cgaggaagtc gtcataggcc tgcccctgca ggcggcgctc
      361 cggcagaccg atgtagagcg ggtcgttgag gaacgtctga ttctcggtgc ccacgtcgag
      421 catgatcggc agcgtggccg tgggcgggat gccggcgcag gcggtgtaga gcgacagctt
      481 gccgacgggg atgcccatgc cgttggcgcc ctgatcgccc aggccgagga tacgctcgcc
      541 gtcggtgacg acgatgacgc gaatgtcgtc gatgggccag ttcttcagca gttcggcgat
      601 gtcgcccttg tccttggcgg agatgaacag cccgcgcggc cgccggaaaa tgtgaccata
      661 ctgctgacac gcctgcccga cggttggcgt gtagacgatg ggcatcatcg tatccaggtt
      721 gtcgatcagg atgcggtaga agagcgtctc gttgcggtct tcgaggccga tcatgtagac
      781 gtacttctca agatcggtcg gcttcttgta gaagttctcc aggattcggg cggcgtggtg
      841 gtgcagggac agcacgcgcg gcggtaaaag gccgcgcaag ccaaacgcct cgcgctcggc
      901 cttgctgaac cccgtgcccc gattgaaaat cgggttaaac aacagttggg tgccgcgggg
      961 gaaatcttcg ggcagttcgg ccgctgcgtc ggccgccttc gtgttttctg acgacatggc
     1021 tcactccaga atcaaaaatt tggtaaacca acaagtcagt cggcagcaga gtgccaatga
     1081 aagaggattc ggctgtcgac cgggttggga aagtgggagc atccaccatc gggatgcagc
     1141 ctcaagtgta caatgaattc cccgattagg gcaatgagtt ggcataaacc tccgattatt
     1201 tattgcgaac ttgcgcccgt ttcgtgtatg atacagatta tcaaccagac ataactcaat
     1261 tggtggcttc tgccgggagg aaacgatggt cctcgaccgt atcgcgcaaa tagctctcat
     1321 atttttcgga gcgctcgtgc tggtcttgct ggtgctgggg ctggcggttt cctgccgcga
     1381 gacaggggcg gggccgattg ggtcggccac ggttgtcctt gattcggcga cggccgatcc
     1441 ctttgccacg gccacaacca acgccggcgc cgctactgcc acgaccgatc ccttcgctcc
     1501 gacagccgac ccttttgcca cagcgaccac agatgccgga gcagccactg ccacgaccga
     1561 tccctttgcc ccgacagccg acccttttgc cacagcgacc accgacgccg gtgcggccac
     1621 cgcgacgacc gatcccttcg ccccgacggt cgaccccctg gcgacgccga ccctcgcccc
     1681 ggccgcgacc gtcaccattg atccggccgc tcccatagcc accgcgaccg ccgatccggc
     1741 cgcaaccgct gcccccggcg cgacggcaac ccctgaagcg acgactatct ccgccacgcc
     1801 ggcttccggc gccacgccgg cctccggcgc cacgccggcc tccggcgcca cgaccgtcgc
     1861 cccgccgcct tcgggcatgg ttcccggcgc gaccgcccgc cacgccgttt cccgcggtga
     1921 gtgggttttg caaatcgcgc gctgctacgg cgtctcctat ggctcggtgc tgaacgccaa
     1981 ctatctggcc aatcccgact acatcctgcc cggctggatc ctgaccatcc ccgatatcgg
     2041 tcgccagggg cgaattgtcg ggccgccgtg cgtcgtcgat tacaccgtcg tcgccggcga
     2101 tacctgggat tcactggccg cgcgctatca gacgaccacg gccatcctga tgaaggccaa
     2161 ccccggcgcg ctggtcgccg gccgctcgat ctgggtccct cgcgtcccct gagcgcgggt
     2221 gaccattacc gccacgaagg agctattatg gcgaaaaccc tgagccgggc cgcatttgat
     2281 cgtgccgcgc agttcatgcg cgaggaagcg cggccgctgg agcgcgcgct gtttgcctac
     2341 catttcgaga agggcagccg cacgcgcgtg ctggccgccc tcgtccccta tcagaacccc
     2401 gacggcggat ttggccacgg catcgagccg gacatccgct ccggggcgtc gtcggtcatc
     2461 ggcaccgtga ccgcgctcga catcctgcgc cgggtgcggg ccgacgagga cacgccgggc
     2521 ctgccggccg cgctggccta tctggtcgat gcctacgacg tcgaaagcga gcgctggccc
     2581 atcgtgcccc ccgacatcga gaacgccccc cacgcgccgt ggtggagcta tgccgattcg
     2641 accgagaact ttcgcggctt ctgggccaac ccgcgcgccg cggtggtggg ccatctgcag
     2701 caatacctgc gcctgtcgcc gtcgccgttc accgagatcg cccgccacgc cgtcgtcgat
     2761 gacctcatgc gctactcgca gcggatggag atgcacgacc tgctcagctt cctcgacctg
     2821 ctggagaccg acgggctgcc ccgcgaacat tacgagagca tcctcgacaa gctgcgccgc
     2881 gccttgccct actccgtcgt gaccgatccg gcgaagtggg gcggctacgg gctgcggccg
     2941 ctcgaggtgg tctatttccc cggctcgccg ctggcggggg tgctcgatca ggcggtgatc
     3001 gacgccaatc tcgactatct catcgacgag caggagccga acggcagttg ggcgccgaac
     3061 tggacatggg acttcatcga ccccttcgcc tgggacgcgg ccgagcgcga atggcgcgga
     3121 gtcctcaccc tgcgcaagct gcgcaccttg cgggcctacg gccgcattga ggggctgtag
     3181 ctccgcgacg gaacgttagc catgaccggc ttcggcgaat gggatgcagt tgagcaggcc
     3241 gaaggcggcc cgccggccac tatccagtca ttgcgagccg acttcgaggc gctgggcctg
     3301 cggccggggc aagtcgtcct cgtccactcc tcgctgaagt cgctgggttg ggtgtccggc
     3361 gggccggtgg cggttatcct ggcgctggag atgacgttgg gggccgcggg aacgctggtc
     3421 atgcccaccc attcggccga cctctccgac ccggcccgct ggcgtaaccc gcccgtgccc
     3481 gaagcctggt ggccgatcat ccgcgacacg atgcccgcct acgaccccga cctgaccccg
     3541 acgcgcggca tgggggcgat ccccgaagcg ttccggcgcg gccgcggcgt ggcgcgcagc
     3601 aatcaccctc aggtctcctt cgctgccaaa ggcccccacg cggcgcagat caccggcggc
     3661 cacccatatg attacggcct gggcgagggt tcgcctctgg cgcggctcta tgaactggac
     3721 gggtgggtgc tgctgctggg cgtggggcac ggcaacaata cgtcgctcca cctggcggag
     3781 aatcgcgcga acttccccgg caagcaggtg gtcgagaacg gcgcgccggt gcgtgtcgac
     3841 ggccgccgcg aatgggtgac gctccacgat attgatttcg acgacgagga cttctccgcc
     3901 atcggcgcgg cgctggagcg ggagacgggc gcggtgcacg tcgggcctgc cgggcgcggc
     3961 gtggcccggt tcatgcgaca gcgggcgctg gtcgatttcg ccgtggcgtg gatggagcag
     4021 caccggcgct aggcgggcga tattcccgcc gccgcgaggc gctcctcggc cagcatcatg
     4081 atccgcagtc gcagacccgc gccgtagcca tagcgggcct tggcgtggat gcggatatcg
     4141 gccgcgctgc ccagcgtgcc gtcgcgggcg atgaccgacc acggctcgat gcaatcgggc
     4201 agcaaatcgg gcgcgtcctg ggccagcatc aacagctcgg ccatcgcctc cggcaaatcg
     4261 gccgccgcca ccttgacgat cacatccacg ccggagtaac tgccgcgcgt gaagttgagc
     4321 agggtgcgca cttccccttg cggcacgttg atcagttccc ctgtgcgggt gcggatgctg
     4381 agcgtgcgca cggtgatatg ctcgatggtc ccttccagct tgtcgcccac gcggaaaatc
     4441 tgtacgttat cgccgatgtt gaagcggttc tcgaagatgt tgctgatgcc gttgagcatg
     4501 tcgccgatgt agtcgcgggc ggcaaagccg aaggcgttga tggccacggt gacgacaagg
     4561 gccatattcg tcaggctgaa gaactgcccg gcggccatga gcagggcgac ggtgaaggcg
     4621 atgaagctga tgaagcctga cgccagttgc agcaccgtct cccgccggcg gatcacgagc
     4681 gtcgccaggt cgttattcat ggccggaggg tcgtcggccg cgggccggcc ggtatcatag
     4741 ccggccatat tcagcagccg cccggccagg cggtcgctga cccggctgac cagccaggcg
     4801 aggccgaaga ccacaaccac aaagacgagt tgcagcggaa agcgaaccaa cgggaagtat
     4861 gtcaccagag cctgggccgt gggcgacgat ccgatgaccg attccagcag agtcacgtcg
     4921 ctgatgttgg cggcgcgcag gacgcccacc accagcgcgg tcagccccaa aatgaccagg
     4981 agcgggatga ggatcagcaa tatcagccgg cgtctcatag cgggttatcg tccttgtctt
     5041 gcgagccttt gccttgcagg gggatgcccg ccgccctgaa ccggtcgtga acgacggcgt
     5101 ggatggccat ctgcgcctcg tcctcccatt tctgatccgt cttgccgaag agaaacacgt
     5161 ccaccgtcgc gccgaggacg ccttccagcc ccatcggctg ccacggctcc atcagttgcg
     5221 gcaccaggtc cggcgactcc ggggccatct ctgtgagcaa ggctacggcc cgacgcaggt
     5281 ctttggcggc gatggggaag gaggcataga cgccgatgaa gttcccgcgg ctgtagttgc
     5341 ggaagatgcg caggtcgccg aacgggatag acgtcagctc ccccgaccgc gcccgaacgg
     5401 agagaaaacg caggtcaacc gattcgaccg tgccctccac cgacttctcg aatagcttgt
     5461 agtagatacg gtcgccgatg gccaggttgt tctcgaaaat gttggtgatg ccacccagga
     5521 gatcgccgat gggcagtcgc gcgccccagg tgagaccgct ggtgagcgcg gcgacgatca
     5581 gcgccagatc gtcgcgggcg atgaactggg ccgcggcgag gagtgtgccg gcaacgatgg
     5641 ccgtgaaggc gatgatgctg gagaggattt gctgcaccgt ccggcggcgc gtgggctgca
     5701 ggacggcggg cagttcgtcg gcgaccccct cgaccgcagt caatcgctcg gccagcggct
     5761 cgcccagcac ccactcggtc aggcggtcgc ggcggcgaaa cacccacagc gcggcagcca
     5821 tggccaggcc gatgaacagg aactggaacg gcagcagcag caaccggacg gtgagcgtgg
     5881 ccagctgcag ctcgaccggt gtgccgtcga acagctccgc caccagcatg cccgccgggt
     5941 tgttgccgga gacgaaggcc aggcccacca tgaggctgat accggccaga gcgagccaga
     6001 gcgggctgag cagccagaaa agaatgcggc gcgcgttcac tatggggtta agtccactcc
     6061 aattctaacc atggt
//
LOCUS       DAOMQG010000191         6067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5535_length_6067_cov_7.868263, whole genome shotgun sequence.
ACCESSION   DAOMQG010000191 DAOMQG010000000
VERSION     DAOMQG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_6067_cov_7.868263"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLC06_10680"
     CDS             complement(<1..499)
                     /locus_tag="PLC06_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90953.1"
                     /translation="MNHRKVLLFIVLLLASVSALSAARRTVPRVAAAPFEGIAAPRLT
                     SHVLIKLAASESAVAGSAAIGLPGRLEHVFGSWYRLYPPSGETADSLLMTLAGSPAVE
                     RVEPDYPVRLDAAPQFDAAPQFDAAPPNDPLYPRQWNMPQVQLEQAWAATTGLDANTG
                     TGVRLA"
     gene            complement(681..1094)
                     /locus_tag="PLC06_10685"
     CDS             complement(681..1094)
                     /locus_tag="PLC06_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRO90954.1"
                     /translation="MTDLQQDAIHYILTHYRTIAAVGFNGRDRTKPAYYTPAYLKGQG
                     FTVIPIGRGLTEGLGRAAYPSLSDAPPGVEVALLFLSPRRVGPAVDEAIAVGAKAVWL
                     PIGVVDEEAAARARAAGLWVVMDRCMMVEHKYLDL"
     gene            complement(1175..1864)
                     /locus_tag="PLC06_10690"
     CDS             complement(1175..1864)
                     /locus_tag="PLC06_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRO90955.1"
                     /translation="MNNEVLSYQDMCQQEGLSLQRGMNFRVDGGYSIILMSQRRNAPY
                     HDRIEDDGATLIYEGHNEPKRDGLIDPKGVDQPATNHRGTLTENGKFAQAAQRYVSGE
                     TPPERVKVYEKIKQGIWSYNGLFHLIDSWIESDGRRMVYKFKLVATEDDSSTDIQETT
                     DLGTNRLIPSAVKLEVWKRDGGRCVQCGANDNLHFDHIIPYSKGGTSLRAENIQLLCA
                     RHNLAKRDRIE"
     gene            complement(1899..2903)
                     /locus_tag="PLC06_10695"
     CDS             complement(1899..2903)
                     /locus_tag="PLC06_10695"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRO90956.1"
                     /translation="MDFLSAAQVVLERHGRPLHYKDIARHALDDGLITPTGMTPEATM
                     GSRLYVDTKKDESRFERAGKGRFSLKKKTKRDDIAKRVEEINQQTRQILRKRLLEMPA
                     DRFEILISELLIAVGFEEPSVQVTSYSGDGGIDVRGVLNAGGVTSVNAAVQVKRWKKN
                     VQTNVVRELRGSLTTHEQGIIITTSDYSAGARTEASAVGKVPISLLNGSELIDLLVKH
                     SIGVNKEPHTVITLDEEWWNEANGDVVVEVVLPVEEIMERETLVEFPLTVRAMNSPHV
                     VAMLFNRQGRMEFNHTQYGTPSSAGKVAPGWKSCNGWRYWQYQHPESKEWRIIDELRG
                     "
     gene            complement(3055..5328)
                     /locus_tag="PLC06_10700"
     CDS             complement(3055..5328)
                     /locus_tag="PLC06_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRO90957.1"
                     /translation="MKRLALLVGLVLLVALAACNAQPAPTPTAVVEAVVPTEAAPTEV
                     PPTAEPAPAEPVAPAIVADPGLIDTLWVWVSRDAAEPLDVANPHNYNLLFNADGTFFA
                     GLDCNRGTGTYNTPGDGTISIALGPTTMAMCADDSLANQMIAMLGSAASYAYEEEGEV
                     VAFTMADGSVDRYRKADTVPSVGAMPPAGDADTALVGRVWQWAGRIAGDQVTTVDSPE
                     DYSLTFNDDGSFYARLDCNNGGGSYTAADGTLTMSGVRTTLALCPDGSMAGEMSTAFE
                     QPLAYAIDGRTLTLTHGDGTIDTYTDAAPSLVGTTWQWLGTTTAKEPITVADSSRYTV
                     EFLADGSAVIKADCNNVIAQYTAVDGAMTITPGPSTLVACPEDSQADVFTQQISAAAI
                     YFFMDGDLYIDLFADGGTMRFGELPTVDLPTPEAGVPTGTVNAPDGVFLRTGPDTSFP
                     SVGVAAQGDSGTLIGISEDSRWYVVDAPSRADGRVWVFAEFVDATGAEGLPVVPNPAP
                     VNVLVGPVWQWAYTTTPVETIRSADPTRYTVQFMADGTAAIQADCNRAVVQYITDGQI
                     ITLTPGPMTLAACPEDSQGDLFMQQLSNAAIFFFQGGELFIDQTASSGTMRFVTGATS
                     PEANGGGDATTVTGLPFRVVSFGPLGAETPILDGTMIDALFDQAGNRVSGTAGCNNFF
                     GPMNTASGAFKLGPFATTMMMCEENVMAQEAAFLAALEGATSFQWMVQPNAFITQGQI
                     NYTLADGTTGVINFVSP"
     gene            complement(5619..5852)
                     /locus_tag="PLC06_10705"
     CDS             complement(5619..5852)
                     /locus_tag="PLC06_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HRO90958.1"
                     /translation="MITLYDTHKRRLGTITDGQLQFLMDSMEEESTTDQDYYLESATL
                     DYLEARGAEHELILFLRGVLDGHEGLTIRWERE"
BASE COUNT         1154 a         1808 c         1852 g         1253 t
ORIGIN      
        1 tggccagccg cacgcccgtg ccggtgttgg cgtcgaggcc ggtcgtggcg gcccaggcct
       61 gctccagctg cacctggggc atgttccact ggcgaggata gagcggatcg tttggcgggg
      121 ccgcatcgaa ctgcggggcc gcgtcgaact gcggggccgc gtcgagccgc accgggtagt
      181 ccggctccac ccgctccacg gctggcgaac cggccagcgt catgagcagc gaatcggccg
      241 tctcgcccga cgggggatag agccggtacc atgacccgaa gacgtgttcc agccggccgg
      301 gcaacccaat ggcggccgat ccggcgacgg ccgattcgga cgccgccagc ttgatcagca
      361 cgtgggatgt caggcgcggc gcggctatac cctcgaacgg ggcggcagcc acgcgcggca
      421 cagttcggcg ggcggccgac aacgccgaca cgctcgccaa caggagaaca ataaagagaa
      481 gcactttgcg gtggttcaca ggtcattcgc tcctgagtca aacagatgcc ggttgcaggc
      541 gtcatctatc tgtcggatgg ccccgggcac ggggcggccg ggcggtggtc cctggctctt
      601 ctttttcgat gggcgccggg cttacttctt tcctgcctgc aagtcggcgg cttgcgcagc
      661 cggcgcgcta ttttctcgat ctagagatcg agatatttat gctccaccat catacaacga
      721 tccatgacca cccacaaccc ggcggcgcgg gcgcgggcgg cggcctcctc atccaccaca
      781 ccgatgggca gccacaccgc tttcgcgccg acagcgatgg cctcgtcgac cgccgggcca
      841 acgcggcgcg gcgacagaaa gagcagcgct acctccaccc ccggcggcgc gtcgctcagc
      901 gagggatacg ccgcccgccc cagcccctcg gtcagcccgc ggccgatggg gataactgtg
      961 aacccctgcc ccttcagata ggcgggggtg tagtaagccg gtttggtgcg atcgcggccg
     1021 ttgaatccca cggcggcgat ggtgcgatag tgcgtcagga tatagtggat ggcgtcttgt
     1081 tggagatcgg tcatagcaat gctgcgactt gctgcgcgaa atagggccag cgtgccggaa
     1141 gggataagga gtgtgtaggg attaggagta ataactattc gattcgatct cgcttggcta
     1201 gattgtgtcg tgcgcaaagt aattggatat tctctgctcg taatgaagtg ccacccttag
     1261 aatagggaat aatgtggtca aaatgcaagt tatcgttagc tccacattgt acgcaccggc
     1321 cgccgtcccg tttccatact tctaacttga cagccgaggg tatcaaacgg tttgttccta
     1381 gatcagttgt ttcctgtata tcggtacttg aatcgtcttc tgttgccacg agcttaaatt
     1441 tatagaccat ccgccgaccg tcactttcaa tccaggaatc aattaaatga aatagaccat
     1501 tatatgacca aatcccctgc ttaatttttt cgtacacttt gacccgctct ggaggggttt
     1561 ctccactcac atatcgttga gcggcttgag caaattttcc attctcagtt agcgtgcccc
     1621 ggtgattggt tgcgggttga tccacaccct ttggatctat caagccatca cgtttaggct
     1681 cgttatggcc ttcgtagatt agcgtagcac catcatcctc aattcgatca tgatatggtg
     1741 catttcgccg ctgcgacata agaataatcg agtagccacc atccacgcgg aaattcattc
     1801 cccgttgcaa acttaaaccc tcttgctggc acatatcctg ataggaaagg acttcattat
     1861 tcataagata agattttccc ctctactttg tttaattgtc agccgcgtaa ttcatcaatt
     1921 atccgccact ctttgctctc gggatgctgg tattgccaat atcgccaacc attgcaggat
     1981 ttccagccag gggcgacttt ccccgcagac gagggcgtac catactgcgt gtggttgaac
     2041 tccattcgtc cttgtcggtt aaacagcatt gcgacaacat gtggtgaatt catcgcccgc
     2101 acggtcaatg gaaactccac cagggtttca cgctccatga tttcttcaac cggcagcacc
     2161 acctcgacga caacatcgcc attggcctcg ttccaccatt cctcatccag agtgatgacc
     2221 gtatgtggtt ctttgttaac gccaatagaa tgtttcacca gcaaatcgat caactctgag
     2281 ccattcaaaa ggcttatggg cactttccca actgcactag cttcggtccg agctcctgca
     2341 gaataatcac tcgtagtgat gatgatgccc tgttcatggg tggtaaggga gccacgcagt
     2401 tcgcgaacga cattcgtttg cacattcttc ttccatcgtt tgacctgcac agccgcgtta
     2461 accgacgtta cgccaccggc attcaatacc ccccgcacat caatgccacc atctccacta
     2521 taactcgtaa cctgaactga aggttcctca aacccgacgg cgatcaataa ttcactaata
     2581 agtatctcaa accggtcagc cggcatctcg agtaggcgct tgcgcaaaat ctgtcgcgtc
     2641 tgctggttta tttcctctac acgcttggcg atgtcatcgc gttttgtctt tttcttgagc
     2701 gaaaatcgcc cttttcctgc tcgctcgaag cgggactcgt cttttttggt atccacatac
     2761 agccgcgaac ccatcgtggc ctccggcgtc atgccggtag gcgtgatcaa accatcgtca
     2821 agtgcgtgcc tggcaatatc tttataatgt agcggccgcc catgacgttc aagaactact
     2881 tgcgcagcgg ataaaaagtc catcgtggat tttcctgggt gaggtatagc ctctctagag
     2941 tatcatatcg tcaatctggg tcaaagatac cccaacaaaa aaagcccgcc ccccaaccgg
     3001 ggagcgggcc tctcttcact cgtatctcgt atctcgtatt tcgtatctcg aaatctacgg
     3061 cgaaacgaag ttaatcacgc ccgtcgtgcc gtcggccagg gtgtagttga tctgcccctg
     3121 ggtgatgaac gcgttgggct gcaccatcca ctggaagctg gtcgcgccct cgagcgccgc
     3181 caggaaggcg gcttcctggg ccatgacgtt ctcctcacac atcatcatcg tcgtggcgaa
     3241 cgggccaagc ttgaacgcgc cggaagcggt gttcatcggg ccgaagaagt tgttgcagcc
     3301 cgcggtgccg ctgacgcgat tgccggcctg atcgaacagg gcgtcgatca ttgtgccgtc
     3361 gaggatgggg gtctccgcgc ccagcgggcc gaaggacacc acgcggaacg gcagaccggt
     3421 cacggtggtg gcgtcgccgc cgccattcgc ctcaggcgaa gtggcgccgg tcacaaagcg
     3481 catcgtgccg gacgaagcgg tctggtcgat gaacagttcg ccgccctgga agaagaagat
     3541 ggccgcgttg ctcagctgct gcatgaacag gtcgccctgc gaatcttcgg ggcaggccgc
     3601 cagcgtcatg gggccgggcg tgagggtgat gatctggccg tcggtgatat attgaaccac
     3661 cgcgcggttg caatcggcct gaatcgccgc cgtgccgtcg gccatgaact ggaccgtgta
     3721 gcgcgtcggg tcggccgagc ggatcgtttc gaccggcgtc gtcgtatagg cccactgcca
     3781 cacggggccg acgagcacat tcaccggcgc gggattgggg acgaccggca ggccctcggc
     3841 gccggtcgcg tcgacgaact cggcgaacac ccagacgcgg ccgtcggccc ggctgggagc
     3901 gtcgaccaca taccagcggc tgtcctcgct gatgccgatc agcgtgccgc tatcgccctg
     3961 ggcggccacg cccaccgacg ggaagctggt gtcggggccg gtgcgcagga acacgccgtc
     4021 gggcgcgttc acggtgccgg tcgggacgcc cgcttcgggg gtcggcaggt caacggtcgg
     4081 cagctcgcca aagcgcatcg tgccgccgtc ggcgaacagg tcgatgtaga gatcgccatc
     4141 catgaagaag tagatggccg cggcgctgat ctgctgggtg aagacgtcgg cttgcgagtc
     4201 ctcggggcag gcaaccagcg tgctggggcc gggggtgatg gtcatggcgc cgtccacggc
     4261 cgtgtactgg gcgatgacgt tgttacagtc ggccttgatg accgccgagc cgtcggccag
     4321 gaactcaacc gtataacggc tgctgtcggc cacggtgatc ggctctttgg ccgtcgtcgt
     4381 gcccagccac tgccaggtgg tgccgacgag cgacggggcg gcgtcggtgt aggtgtcgat
     4441 ggtgccgtcg ccgtgggtca gcgtcagggt gcggccgtcg atggcatagg ccagcggctg
     4501 ctcgaaggcg gtggacatct cgccggccat cgacccgtcc gggcaaaggg ccagcgtggt
     4561 gcgcacgccg ctcatggtca gcgtgccgtc agcggcggtg tagctgccgc cgccgttgtt
     4621 gcagtcgaga cgggcataga aggagccgtc atcgttgaag gtcaggctat agtcctcggg
     4681 ggaatccacc gtggtgacct ggtcgccggc gatgcggccg gcccattgcc agacgcggcc
     4741 gacgagcgcc gtgtcggcgt cgcccgcggg gggcatggcg ccaacagacg ggacggtgtc
     4801 ggccttgcgg tagcgatcga cgctgccgtc ggccatggtg aaggccacca cctcgccctc
     4861 ttcctcatag gcgtaggacg cggccgagcc gagcatggcg atcatctggt tggcgaggga
     4921 gtcgtcggcg cacatggcca tcgtcgtcgg gccgagcgcg atggagatcg tgccgtctcc
     4981 gggggtgtta taggtgccgg tgccgcggtt gcagtccagc ccggcgaaga aagtgccgtc
     5041 ggcgttgaac agcaggttgt agttgtgggg gttggccacg tcgagcggct cggcggcgtc
     5101 gcggctcacc cacacccaca gggtgtcgat gagaccgggg tcggccacga tggccggggc
     5161 gaccggctcg gccggggcgg gttcggccgt cggcggaacc tcggtcggcg cggcttcggt
     5221 cggaacaacc gcctcaacaa cggcggtggg ggttggtgcg ggctgcgcat tgcaggccgc
     5281 cagcgcgacc agcaagacaa gaccaaccaa caaagcaaga cgtttcattt tgttctattc
     5341 tccttgcaaa ttgcaaataa aaatcctttt tgcctcgaac gatgcaccca tcagcataac
     5401 gtcgccggat atgtgccggt tccgccactc aggaatcgct cattttgaaa gaaaaagggg
     5461 gaccttcagc ttgaaatagg ggtttttact ccccaaacag ccattataac agattttttc
     5521 gcttgggggc aagccggcga gacagcgcac aacgcccggc gggcagccgt cgggcgttcg
     5581 tgggcgtcag gttgtggaat cggggcggga agcgggggtt attcccgttc ccagcggatc
     5641 gtcaggcctt cgtggccgtc gagcacgccg cgcaggaaga gaatgagttc gtgttccgcg
     5701 ccgcgagcct ccagatagtc gagcgtggcc gactccagat agtagtcctg gtcggtggtg
     5761 gactcttcct ccatgctgtc catgaggaac tggagctggc cgtcggtgat ggtgccgagg
     5821 cggcgcttgt gggtgtcgta gagtgtgatc atgtgaatat ctcctttggg aagagtatac
     5881 aaaaacgagt gacgagtgac gagtgatgag tgacgaatga cgactcctgt cgtcggacga
     5941 caggagtcgt cggacgaaat ggagagcgcg tctctccccg gccccctttc cccctttctc
     6001 cctttcttcc caccccttgc caccctcccc ccacaacgct atgatccgtc cgtgcggcat
     6061 catccca
//
LOCUS       DAOMQG010000192         6061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5543_length_6061_cov_10.514985, whole genome shotgun sequence.
ACCESSION   DAOMQG010000192 DAOMQG010000000
VERSION     DAOMQG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5543_length_6061_cov_10.514985"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..131
                     /locus_tag="PLC06_10710"
     tRNA            56..131
                     /locus_tag="PLC06_10710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89..91,aa:Ala,seq:cgc)
     gene            complement(480..683)
                     /locus_tag="PLC06_10715"
     CDS             complement(480..683)
                     /locus_tag="PLC06_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90959.1"
                     /translation="MNAHQKSTGFITVTPTMRSLIENVIEDLPLLLDEIDGDENLEPD
                     LAGFDSSTMDDREKDVNSQNFWD"
     gene            complement(680..820)
                     /locus_tag="PLC06_10720"
     CDS             complement(680..820)
                     /locus_tag="PLC06_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90960.1"
                     /translation="MISQDTLSAADDIEEAVEAWRNATPEARAEAIRILRAAVLAGGR
                     KR"
     gene            complement(817..1149)
                     /locus_tag="PLC06_10725"
     CDS             complement(817..1149)
                     /locus_tag="PLC06_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90961.1"
                     /translation="MSDATLLKLEEAFNANSDRELQAGDKVRKLEAEFDRLRKRMRKA
                     ERREDHRTREGARLFKKVMETRADSLEGMFAKVRIRERWNTDEETSEVAVLKSLVADM
                     KAMMEARS"
     gene            complement(1146..1670)
                     /locus_tag="PLC06_10730"
     CDS             complement(1146..1670)
                     /locus_tag="PLC06_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90962.1"
                     /translation="MNRLTRRSLLSSAPVFGFTAIAPTTMTAAAALDYGRSDAELIRL
                     GGEFDRLRAEYYPLRDEAHRLHHLWIDTCQARGVRLGSEAAWVIADEVGVVAADHREE
                     PVQIAFDATVTAILAAPAATLVGLAVKAKVIRLEVLAHAVEPTEAEYRAMDWGDETVI
                     RFCFEIERMTGGAA"
     gene            1657..1839
                     /locus_tag="PLC06_10735"
     CDS             1657..1839
                     /locus_tag="PLC06_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90963.1"
                     /translation="MSLFIRLSPGGNRLTVSPAGDHDERVFMLDLYPPVATVDTLHRS
                     GEVFGSNANLGHGRLG"
     gene            1995..3488
                     /gene="istA"
                     /locus_tag="PLC06_10740"
                     /pseudo
     CDS             1995..3488
                     /gene="istA"
                     /locus_tag="PLC06_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            3496..4230
                     /gene="istB"
                     /locus_tag="PLC06_10745"
     CDS             3496..4230
                     /gene="istB"
                     /locus_tag="PLC06_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRO90964.1"
                     /translation="MERSQILDMMGTLKLYGMRSAYDETMAAGIKRQHEPPRIVGDLL
                     QSEIAEKQARSIKYQITIAKLPLAKDIDDFDFTGTPINEGLVRQLATGAFLAEQHNIV
                     LVGGTGTGKSHLSIALARALIRNGARGRFYNVVDLVNRLETEARNGKQGRLADFITRL
                     DFVIMDELGYLPFAQAGGQLLFHLISRLYERTSIIVTTNLAFGEWPAVFGDAKMTTAL
                     LDRLTHHCEIIETGNESWRFKNRSQS"
     gene            complement(4355..5464)
                     /locus_tag="PLC06_10750"
     CDS             complement(4355..5464)
                     /locus_tag="PLC06_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRO90965.1"
                     /translation="MNWSIPMSYPSWKEIRQGTIDNTYSFMNPRYFPMISEDMPTFMG
                     RPHATRPEDLIGADVVIIGAPYVAGWGGYMGVGAEEWLAAPKRIRQQSIRYGSGYIQE
                     LDLDVFEHLNVVDYGDADIPSECLVKPTVENVLRAQAAVEAKVNDAMEAGAVPVVIGQ
                     NSPCGSFAIAKVVSERTKGNVGCVSLDTHWDIAPLDKLTMDPRIAGSDSWKYKTYELL
                     HNFDQKHLVEIGERGMLENKEMVRMFLEKGTHFYPMWKVRSELGIEGLVNELRHAYEG
                     TQAVYCHWDMDILGGAGPAPGDILGELAEPFGMTDYEVIRLAHEVGKRGISALSFICI
                     PPGSAVIYRVLVYVIMFMCAGLAMRKCDKVCQSAS"
     gene            complement(5587..>6061)
                     /locus_tag="PLC06_10755"
     CDS             complement(5587..>6061)
                     /locus_tag="PLC06_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008758594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRO90966.1"
                     /translation="STGVMTAVVFILVAAGAAFAWTISYAQIPQQLLAAVGLENAGPI
                     KALIAINIAFFIGCMFVDSIVVMLIMVPIFSPVIAATGLDPVLVGVLITMQVAIGAAT
                     PPFGVNLFTAVAVFRRPFLDVVRGVPPFIAILLAVTALVAIFPWIALGLRDLAFR"
BASE COUNT         1244 a         1864 c         1733 g         1220 t
ORIGIN      
        1 caactgccgc ttgacagcgc agcgggcggc acttaaatgc gcgcatccgc tggacggggc
       61 tgtagctcag ctgggagagc gcgtcgttcg caatgacgag gtcaggagtt cgatcctcct
      121 cagctccacc agtggcaaac cttacggcgt tgatttacct tcggttttcg ggtcggcttc
      181 atgccttccc cacttccttc cccactttac tcacgcgcgg ccatcccctt tgaggccaac
      241 gggtccgcga aacaggtttc cggataatcg aggcggatga ggatagattt tcaggtacgc
      301 tcagggacgc gagtagaaag ccgacacgcc attcgcctct tgacaccact ccacgctgtc
      361 ctgcgagagg ttcacatcct cagaccaact gatggtttcg ccatagtcgc aggcatcatg
      421 ctccagatca tccgggctgc cggatgacgg cccgcttcat cggtatgtgc ggggcattct
      481 taatcccaga aattctggga attaacatcc ttttcgcggt cgtccatcgt actcgaatca
      541 aagccggcaa ggtccggctc caggttctcg tcgccatcga tctcatcgag aaggagcggc
      601 agatcctcga tgacgttttc gatcaggctg cgcatggtcg gcgtcaccgt gatgaacccg
      661 gtggatttct gatgggcgtt catctctttc gccctcccgc aagcacagca gcgcgcagaa
      721 tccgaatcgc ctctgcccgg gcttccggcg tcgcgttgcg ccacgcctcc acggcttcct
      781 cgatatcatc ggcggcgctc aacgtatcct gactgatcat gaccgcgcct ccatcatcgc
      841 cttcatgtca gcgacaaggc ttttgaggac agctacttcg ctggtttcct cgtccgtgtt
      901 ccaccgctcc cggattctca cctttgcgaa catcccttcc aggctgtcgg cgcgcgtctc
      961 catgaccttt ttgaatagcc gagcgccttc ccgcgtgcgg tgatcctccc tgcgctctgc
     1021 cttgcgcatg cgcttacgga ggcggtcgaa ctcggcctcg agctttctga ctttgtcgcc
     1081 ggcttgtaac tcccggtcgc tgttcgcgtt gaacgcctcc tccagcttca agagagttgc
     1141 gtcgctcatg ccgccccccc tgtcatccgc tcgatctcga agcaaaaccg gatgactgtc
     1201 tcgtcacccc aatccatagc gcggtattct gcctcggttg gttcaacagc gtgggccagt
     1261 acctccaaac ggatcacctt cgccttgacc gcgaggccta ccagcgttgc ggccggagcg
     1321 gccaaaatag cggtcaccgt cgcgtcaaat gctatttgga caggctcttc ccggtgatcg
     1381 gccgcgacaa ctccgacctc gtcggcaatc acccaggcgg cctcactccc gagacgaact
     1441 ccgcgcgcct ggcaggtgtc gatccagaga tgatggagcc tgtgggcttc atcacgaagc
     1501 gggtaatact cagcgcggag ccgatcaaac tctccgccaa gacggatcaa ttcagcgtcc
     1561 gagcgcccat aatccagcgc cgccgcagcc gtcattgtgg tcggcgctat tgccgtaaac
     1621 ccaaagacgg gcgcggatga caagagggaa cgacgtgtga gcctgttcat cagactatct
     1681 cccgggggaa atcggctgac ggtctctccg gcgggcgatc atgacgaacg ggtcttcatg
     1741 ctcgatctgt atccgccagt tgccaccgtg gatacgctgc atcgcagcgg agaggtattc
     1801 ggcagcaacg cgaacctcgg ccatggccgc ctcggctgag ataagcgcgt gggcgaagtg
     1861 ggcgttgttg atgtctttct gtgattaggc atggaataag gaccccgtta gcggggtgat
     1921 cggcttccaa aagggacccc ttctgacgat ggggttacaa cagcctccgg tttcatcgga
     1981 ggctttttat ttggatgctt gttgtggaga caattttgaa gatacgccga ctttcccggg
     2041 tgcagggcaa gtcgatcaag gcgatctgcc gcgagttggg ggtatcgcgc aaagtggtgc
     2101 gcaaggttct gcgcagcgat gagacggagt tcaaatacga gcgcacccgc caacctcagc
     2161 cgaagctcgg cccctggcgg gatcatctcg atggaatgct gttggacaac gaggccaaat
     2221 cctcccgcga acggctgacg ctgatccgca tattcgagga cctgcgggat ctcggctacg
     2281 agggtggtta cgacaccgtc cgacgttacg cccggacatg ggcgaaagag cgcggatccg
     2341 tgacggcgca ggcctatgtg ccgctctatt acgcgccggg cgaagcctac cagttcgact
     2401 ggagccacga gatcgtcgtg ctgaacggcg tgacgacgac ggtgaaggtg gcgcatgtcc
     2461 ggctctgcca tagccggatg atgtatgtga gggcctatcc gcgcgagacg caggagatgg
     2521 tcttcgatgc ccatgaccgg gcgttcgcct tcttcggcgg cgcctgcacg cgcggcatct
     2581 atgacaacat gaagactgcc gtggagacgg tcttcgttgg caaggagcgg caatataacc
     2641 gccgcttcct tcagatgtgc agccactacc tgatcgagcc ggtcgcctgc acgccggcgt
     2701 caggatggga gaagggccag gtcgagaacc aggtcaatct ggtacgggaa cgcttcttca
     2761 cgccgcggct gcgcttcaaa tcctacgatg agctgaacgg ctggcttctg gacaagtgca
     2821 tcagctatgc ccgcgcccat aagcatgtcg atcagacgga gcgcacgatc tgggaggtgt
     2881 tcgatgccga gcggccgcac ctggtcggct atccgggcag gttcgatggc ttccatgccg
     2941 tgcaggcctc gatcggcaag acctgcctcg tgcgtttcga caacaacaaa tactcggttt
     3001 tgtcgaccgc ggtcggacgc ccggccgaga tccatgccta tgccgaccgc atcgtcatcc
     3061 ggcaggatgg cgtcgagatc ggccagcacg aacgctgctt tggccgtggc gagacgatct
     3121 ataatccatg gcattacgtg ccggtcttga cgcgcaaacc gggagcactg cgcaacggcg
     3181 ccccgttcca cgactgggtt ttgcctgcgg ccatggagaa ggtgcgccgc cggctgaagg
     3241 ccatgcatga cggtgaccga cagatggtgt cgattctcga atgtgtcggt ctggacgggc
     3301 tccccgccgt cgagacggcc tgccaggagg cgctcgatca gggcgtgttc tccgccgccg
     3361 tcatcatcaa catcctggcc cgcaagcgtg atccgcagcc ggccgccatt ctttccatcc
     3421 ccgaagcgct tcgcctgacc catgagccta tggccgattg cgcccgctat gacagcctca
     3481 ggagggcaag ctgatatgga acggtcccaa atcctcgaca tgatgggcac gctgaagctc
     3541 tacggcatgc gcagcgccta cgacgagacc atggctgccg gcatcaaacg ccagcacgag
     3601 ccgccccgca tcgtcggtga cctcttacaa tcggagatcg ccgagaaaca ggcgcgctcc
     3661 atcaaatacc agatcaccat cgccaagctc cccttggcca aggatatcga cgacttcgat
     3721 tttaccggca ctccgatcaa tgagggattg gtgcgccagc tcgcaaccgg ggcattcctg
     3781 gccgagcagc acaacatcgt tctggtcggc gggaccggca cgggaaagtc gcatctgtct
     3841 atcgcgctcg cccgtgccct catccgcaac ggcgcgcgcg gacgcttcta caacgtcgtc
     3901 gatctcgtca atcggctcga aacggaagcg cgcaatggca aacaggggcg gctggctgac
     3961 ttcatcacca ggctcgactt cgtcattatg gacgaactcg gctatctccc cttcgcccag
     4021 gccggcggcc aactgctctt ccatctcatc agcaggctct acgagcggac atcgatcatc
     4081 gtcaccacca acctggcgtt cggcgagtgg ccagccgtgt tcggggacgc aaagatgacc
     4141 accgcgctgc tcgatcgtct cacacatcat tgcgagatca tcgagacagg aaatgaatca
     4201 tggcgcttca aaaaccgctc tcaaagctaa agccgagaaa gataacaaaa ccgcgcccgc
     4261 cgaccctgcg ctaaatggag ggctccaggc cggcgggcgc taaccgttgc aaccagaagg
     4321 aggggatctt tttgaaagcc gataaggggt cccgttacga cgccgattga cagaccttat
     4381 cgcattttcg catcgccaat cctgcacaca tgaacataat cacgtaaacc agtacgcgat
     4441 agattactgc ggaccctggt gggatacaga tgaaagacag ggcagaaatc ccccgcttgc
     4501 cgacttcgtg tgcgaggcgg atgacttcat aatctgtcat gccaaagggt tccgccagtt
     4561 cacccagaat atctcccggg gctgggcccg cgccaccaag aatatccata tcccagtgac
     4621 aataaacggc ctgcgttcct tcataagcat gacgcagttc gttcaccaat ccctcgatgc
     4681 ctaattcgga gcgaaccttc cacatcggat agaaatgggt gcctttttca aggaacattc
     4741 gcaccatctc tttgttctcg agcattcccc tttcgccaat ctcaaccagg tgcttctgat
     4801 caaaattgtg taggagctcg taggttttgt atttccagga gtccgagcct gcaattcgcg
     4861 gatccatagt cagcttatcc agcggggcga tgtcccaatg tgtatcaagc gagacgcagc
     4921 caacgtttcc tttggtgcgt tcggaaacaa ctttcgcgat cgcaaaggat ccgcagggac
     4981 tgttctggcc gattacgacc ggcactgccc cggcctccat ggcgtcattg accttggcct
     5041 cgaccgcagc ttgtgcccgg agcacgtttt ccactgtcgg cttcacaaga cattccgaag
     5101 gaatgtcggc atcgccatag tcaacgacat tgaggtgctc gaacacatcc aggtccagtt
     5161 cctgaatata tcctgagcca tagcggatcg attgctgccg gatgcgcttg ggcgcggcaa
     5221 gccactcttc cgcgcccacc cccatgtagc caccccagcc ggctacataa ggcgctccga
     5281 tgatcacgac gtctgctcca atcaggtctt cgggtctggt cgcgtgcggc ctccccatga
     5341 atgtcggcat gtcttccgag atcatcggga aatagcgggg gttcatgaag gagtaggtgt
     5401 tgtcgatggt gccttgtcga atctctttcc aactcggata tgacatcggg atgctccagt
     5461 tcatcggccc gtcaacgcct atgcgtttga cattttctgt tccaccaaat cgcgacagat
     5521 gcgatcaaag ggagcgcgcg tcagtgccga ggtgacatag acgcgcgcca gttcttcctg
     5581 gacaggttat cggaaagcga gatcgcgcag tccgagcgca atccagggaa agatagcgac
     5641 gagcgccgtc accgcgagaa ggatcgcgat gaagggcggc acgccccgga ccacgtccag
     5701 gaagggcctt cgaaacaccg ccactgcggt gaagaggttg acgccgaagg gcggggttgc
     5761 ggcaccgatg gcgacctgca tggtgatgag cacacccacc agaaccgggt cgaggcctgt
     5821 cgctgctatc accggcgaga agatcggcac catgatcagc atgacgacga tcgagtcgac
     5881 gaacatgcag ccgatgaaaa acgctatatt gatcgcaatg agagccttga tcggacccgc
     5941 attctcaagc ccgacggctg ccagaagctg ctgagggatt tgggcgtagg agatcgtcca
     6001 ggcaaaggcg gcgccagcgg cgaccagaat gaacacgacc gccgtcatca cgccggtcga
     6061 g
//
LOCUS       DAOMQG010000193         6049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5556_length_6049_cov_7.038872, whole genome shotgun sequence.
ACCESSION   DAOMQG010000193 DAOMQG010000000
VERSION     DAOMQG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5556_length_6049_cov_7.038872"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..769)
                     /locus_tag="PLC06_10760"
     CDS             complement(113..769)
                     /locus_tag="PLC06_10760"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRO90967.1"
                     /translation="MQILYFVLAGLIGYLCGAIPFGYIYVRLIKGEDLRKIGSGRTGG
                     TNSLRAAGLGVGVLTSLSDVLKGAASVWLTTWLLGDAAGPATLPWLQATAGVLSVVGH
                     NWSIFLKFGGGAGTGPNVGWSSAIWLWVLPIAFLVMGGMLYFVGMASVASLAMAAVTI
                     IAFAWRYLTGVDSTAAYLVGSIAAGLVVTWALRPNIKRVLNGTERLVGRRAKQREQQK
                     "
     gene            complement(882..1298)
                     /locus_tag="PLC06_10765"
     CDS             complement(882..1298)
                     /locus_tag="PLC06_10765"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRO90968.1"
                     /translation="MDEIHLPAGLHEAMIRRASDGYPEEVCGLLAGVVGGRLGRAVSH
                     YPVENALHSPVAYEMEPSAQVRAMIAIESEGLELVAIYHSHPTGPAAPSRSDIAQALY
                     PDTAQIIISLADRSQPTASAYLIRDDRATEIRLVMI"
     gene            complement(1399..1842)
                     /locus_tag="PLC06_10770"
     CDS             complement(1399..1842)
                     /locus_tag="PLC06_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HRO90969.1"
                     /translation="MSKKIVVLLTVLAIGLLMAACGGGGGGTESSGGGAAAPAPVSLS
                     FTGLDTFHYDPDSASVPAGAQVTVDFSNGGALEHDWMLVPAGTDVNALTVDKAVSPEA
                     HSGVIAAGQSKTFTFTAPAAGTYQIVCTVAGHAPAGMVGTFTVTE"
     gene            2152..5220
                     /locus_tag="PLC06_10775"
     CDS             2152..5220
                     /locus_tag="PLC06_10775"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRO90970.1"
                     /translation="MSASNMAPARRRSLPWWWFIPAVVAACWFVAGLALSRLLFEGLF
                     PAWPLMGRPAGALALGVTLAGLGVAAAMLVAKRAAVPDPTHLAASFLPLLINLAWLFD
                     PAVNPARGRFLFAAGLWLAAVLIIWTIAGDQDRRWRWLGPLAVAVALLPIYLATMSHA
                     VGAADTFEFQVVAPQLGIAHPTGYPLYLLLGKAFSLLPLGTQAWRLNLASAVYAVAAA
                     MVVFRLGLEIFRRPLPALAGAVALGLVPIYWGQAIVAEVYTLHALVVAVALWLMTRLI
                     GEAAPAGQGPMTPQDRKMMVGLAFVLGLGLTNHLTTVFLLPPAGLALLFNLWPVIGRR
                     RAAGERPTSRRRSLIPLFFQLLIAFAVPLVLFAYLPLRWQAVNNEPMGLGRFVEWVAG
                     GRFQGALQWGAWLRDPARRAIVGRLLLDAWGWFYLAPAAVGLAWLFLRRRRTALVLAV
                     AAAGFTFYALNYYVPDLAVFLIPTHVVLAVWVGAGMMGVETGFSEKNPVSVWKTIIFV
                     VALLPAVLGAGERWAGIDQSRRDGGELWARLALTQPLAQGAAILADSEKIAPLYYLQQ
                     AEGLRPDLTIMVLPDEAAYRAELDARLAAGQAVYLARYLPGLAGIYHLRSAGPLVEVS
                     REPLAELPDGVAAADRLFGPLRLSGYQLSTTTDDVLLTLYWTPNRPLVEGEETPVVYL
                     RWADGETVVGGAHPAGDAYPVNAWRPEEIVPDFHRLPLPAADCGAAECELTVEAAVAP
                     RFTAMDALDWQPVASVVATRRSGPVGAARRMLFDGFALDGVAFPTSTRPGAALALRYS
                     GFGDGENLRFALVPAHAVSSLVFPANRGPASRPAANGSVAFAAGVDPATETGPHALVA
                     WSTGEAGAMCGWLASPMTGCVLAEIAVSGAVLPAGATNFDDKIALVEAAVDAAGLVPG
                     GQLPVTLTWQGLAEMSEDYTVFVQVLDAVDRIVGQVDAWPVQGTFPTSQWRPGETVID
                     PYLVQLSPDLEPGDYRVIVGFYLLATGQRLAVVDAAGNAVDDRVELRIRN"
     gene            5231..5878
                     /locus_tag="PLC06_10780"
     CDS             5231..5878
                     /locus_tag="PLC06_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRO90971.1"
                     /translation="MGKIILIAGNSGVGKTTLANALCRLRPFVGALEQHVERPFQALM
                     AADPTRYALANQLDYFLLRAEQERAIRNGPVDGLIDGGLDLDYHAFTQLFTTKGYLSA
                     AEADLLRRLHAALRAALGPPDLVLYLIAPAAVVEARYARRGRPLEIAQRDDLALLEQF
                     VSGWVAGITDAPVITIDAAAEDCYSAERLAALLAQMDELVVNQESGITNQELKRG"
BASE COUNT          950 a         1806 c         2125 g         1168 t
ORIGIN      
        1 tactgatctt actgatctta ctgatcttac tgatcttact gatcttactg atcttactga
       61 tctactgatc ttactgatct actgatctta ctgatctact gatcttactg acctacttct
      121 gttgctctcg ctgtttcgcg cgccggccga cgaggcgctc cgtgccgttg aggacgcgct
      181 tgatgttggg gcgcaaggcc caggtgacga ccagcccggc ggcgatactc ccgacgaggt
      241 aggcggcggt cgaatcgaca ccggtcaggt agcgccaggc gaaggcgatg atggtcacgg
      301 cggccatggc gagcgaggca accgaggcca tgcccacgaa gtagagcatc ccgcccatga
      361 ccagaaaggc gatgggcaac acccatagcc agatggccga cgaccagccc acattggggc
      421 ctgttcccgc gccgccgcca aatttgagga agatggacca gttgtggccg acgaccgaca
      481 ggacgccggc ggtggcctgc agccacggta gcgtagcggg gccggcggcg tcgccgagca
      541 gccatgtcgt cagccagacg gaggccgcgc ccttcaggac gtcgctcagt gaggtcagca
      601 ccccgacgcc cagcccggcc gcccgcagcg agttcgtccc gcccgtgcgg ccgctgccga
      661 tcttgcgcaa gtcttccccc ttgatgaggc gaacgtagat gtagccgaag gggattgccc
      721 cacagagata gccgataaga ccggccagaa caaaataaag gatttgcatc gttaaaaagc
      781 tcccttgccg ggtgagatac atctataaaa cacgcaaccc ggcttttggt tgcacggtgt
      841 gggtagtata gaaaccgggt ttctgccgag aaccccggtt tctagatcat tactagccga
      901 atctctgtcg cccgatcatc gcgaatcagg taggcactcg cggttggctg tgaccggtcg
      961 gcgagcgaga tgatgatctg cgcggtgtcg ggatagaggg cctgggctat gtcgctgcgc
     1021 gacggcgcgg cggggccggt cgggtgcgag tggtagatgg ccaccagctc cagcccttcc
     1081 gactcgatgg cgatcatcgc ccgcacctgc gccgatggct ccatctcgta ggccacgggg
     1141 ctatggagcg cgttttcgac cgggtagtgg ctcacggcgc ggccgagccg accgccgacg
     1201 acaccggcca gcaagccgca aacttcttcc gggtagccgt cgcttgcccg gcgaatcatt
     1261 gcctcatgca ggccggcggg caggtgaatt tcgtccatgg gatgaccgcg gggaatggta
     1321 gccggacgac cggttaaatg caaacgggcc gcaccttcgt gcggcccgtt gagttgagcg
     1381 gctaatcgac gcctggggct actcggtgac ggtgaacgtg ccgaccatgc ccgcgggggc
     1441 gtgtccggcg accgtgcaga cgatctggta ggtcccggcg gccggggcgg tgaaggtgaa
     1501 tgtcttggac tggccggccg cgatgacgcc ggagtgggct tcgggcgaga cggctttgtc
     1561 gacggtcagg gcgttcacgt cggtgccggc ggggacgagc atccagtcgt gctccagcgc
     1621 gccgccgttg ctgaagtcga cagtgacttg tgcgcctgcg gggacggagg ccgagtcagg
     1681 atcgtagtgg aacgtatcca ggccggtgaa ggacagggag acgggtgcgg gcgcggccgc
     1741 gccgccgcca ctgctctcgg ttccgccacc gccgccgccg caggcggcca tcaacaggcc
     1801 aatggcgagc accgtcaata aaacaacgat cttcttggac atgatcatta tctcctacta
     1861 cagggtatat ggaattatgg tcttacggcg ttgtgattcc gtcgtgcaac ttaagcaatc
     1921 ttaagacaaa aatggcgcgc tgtcatcaac taaaatcact tatttatctt gctataaagc
     1981 gggggggagt gacgagtgac gagtgacgag ttgcgagtga cgagcgaaga gccggctgac
     2041 gaagtacgaa agagggcgct cctggtgcgg tcggtcgtcg acggtcgtcg gccgtccgcc
     2101 gatccttcat tcataattcc taattctccc cccggtgtac aataggtggc gatgagtgca
     2161 tcgaatatgg ctccggcgcg caggcgctct ctcccatggt ggtggtttat ccccgccgtt
     2221 gtcgccgctt gctggttcgt ggccggactg gctttgtcgc gcttgctatt cgaggggctg
     2281 ttcccggcgt ggccgctcat ggggcggccg gcgggagcac tggcgctggg cgtgacgcta
     2341 gccgggttag gggtagcagc ggcgatgctg gtggcaaaac gagcggccgt gcctgatccc
     2401 acccacctgg ctgcatcgtt tctgccgctg ctcatcaacc tggcctggct gtttgacccg
     2461 gcggtgaacc cggcgcgcgg ccgttttctg ttcgccgccg ggctgtggct ggcggcggtg
     2521 ctgatcatct ggaccatagc aggcgatcag gatcggcgct ggcggtggct ggggccgttg
     2581 gcggtggcgg tggcgctcct gccaatctat ctggcgacga tgtcccacgc ggtgggtgcg
     2641 gccgatacgt tcgagttcca ggtcgtggcg ccgcaactgg gcatcgccca ccccaccggc
     2701 tacccgctct acctgctgct gggcaaggcg ttctcgctgc tgccactggg cacgcaggcc
     2761 tggcggctga atctggcctc ggccgtctat gcagtggcgg cggcgatggt cgttttccgg
     2821 ttggggctgg agatcttccg acggccgttg cccgcgttgg ccggggcggt ggcgctgggg
     2881 ttggtgccca tctattgggg gcaggcgatc gtcgccgaag tctacacgct gcacgcactg
     2941 gtggtggccg tggcgttgtg gctgatgacg aggctgatcg gcgaggcggc acccgccggc
     3001 caagggccga tgacacccca ggatcgcaag atgatggtcg ggctggcatt tgtgctgggg
     3061 ctggggctga ccaatcatct gacgaccgtc tttctgctgc cgccggcggg gctggccctg
     3121 ctgttcaacc tgtggccggt catcggccgg cgacgggcgg cgggcgaacg ccccaccagt
     3181 cgccggcgct cgctaattcc gctgttcttc cagctgctca tcgccttcgc cgtgccgctg
     3241 gtgctgttcg cctatctgcc gctgcgctgg caagcggtga acaacgagcc aatggggctg
     3301 ggtcgctttg tggaatgggt ggcgggtggg cgattccagg gggcgctgca gtggggagcg
     3361 tggctgcgcg acccggcgcg ccgggcgatt gtcggccgat tgctgctcga cgcctggggc
     3421 tggttctatc tggcgccggc ggccgtggga ctggcgtggc tattccttcg tcgccggcgc
     3481 acggcgctgg tgttggccgt cgcggcggcg ggcttcacgt tctacgcgct caattactat
     3541 gtgcctgatc tggccgtgtt tttgatcccg acccatgtgg ttttggcggt gtgggtgggg
     3601 gcggggatga tgggcgtaga aaccgggttt tccgaaaaaa acccggtttc tgtctggaag
     3661 actattattt tcgtcgtggc gctgctgccg gcggtgttgg gagcagggga gcggtgggcc
     3721 ggtatcgacc agtcgaggcg agacggcgga gaactgtggg cgcggctggc attgacccaa
     3781 ccgctggccc agggagcggc gattctggcc gacagcgaga agatcgctcc gctctattat
     3841 ctgcaacagg ccgagggatt gcggccggat ctcactatca tggtgctgcc cgacgaagct
     3901 gcctaccggg ccgaactgga cgcgcgattg gcagccgggc aggcggtcta tctggcacgt
     3961 tacctgccgg ggctggccgg gatctatcat ttgcgttcgg ccgggccgct ggtcgaggta
     4021 agtcgcgaac cgctggccga attgcccgac ggggtggccg cggccgaccg gctatttggg
     4081 ccgttgcgct tgtcgggcta tcagctctcc acgacgaccg acgacgtcct gctcacgctc
     4141 tattggacgc cgaatcggcc gctcgtcgag ggcgaagaga cgccggtcgt ctatctgcgt
     4201 tgggcggacg gtgagacggt cgtcggcggc gcgcatccgg ccggagatgc ctacccggtc
     4261 aacgcctggc ggccggagga gattgtgccc gatttccaca ggctgccgtt acccgccgcc
     4321 gattgcgggg cggccgaatg cgaattgacc gtcgaagcgg ccgtcgcgcc gcgatttaca
     4381 gcgatggacg cgctggattg gcagccggtg gcgtcggtgg tcgccacccg ccgctccggc
     4441 ccggtgggcg cggcacggcg gatgctgttc gacggcttcg cgctggacgg ggtggcgttc
     4501 ccgacctcaa cccggccggg agcggcgctt gcgctgcgct atagcgggtt cggcgacggc
     4561 gagaacctgc gtttcgcact ggttcccgcc catgccgtga gcagtctggt gtttccagcc
     4621 aatcgcggcc cggcaagtcg cccggcggcg aacgggtcgg tggccttcgc ggccggtgtt
     4681 gacccggcga cagagacggg gccgcacgcg ctcgtggcct ggtcgaccgg tgaggctggg
     4741 gcgatgtgcg gctggctcgc ttcgccgatg accggctgcg tgctggccga gatcgccgtc
     4801 agtggggcgg tgctaccggc gggggcgacc aatttcgacg acaagatcgc gctggtagag
     4861 gcggcagtgg atgccgcggg gcttgtgccg ggcggacagc tgccggtgac gctgacatgg
     4921 caggggctgg ccgagatgag cgaggattac accgtgttcg tgcaggtgct ggacgccgtc
     4981 gatcgcattg tagggcaggt ggacgcctgg ccggtgcagg ggacattccc cacaagccaa
     5041 tggcggccgg gagagacggt gatcgacccc tatcttgtgc aactaagtcc cgatttagag
     5101 ccgggcgact atcgggtgat cgttgggttc tatctgctgg cgacggggca gcggctggcg
     5161 gtggtcgatg cggcggggaa cgcggtagac gacagagtgg aattaaggat cagaaattag
     5221 gaattacgaa ttgggcaaaa tcattctcat cgcgggcaat tccggcgtcg gcaagaccac
     5281 attggccaac gcgctatgtc gcttgcggcc gttcgtcggc gcgctggagc aacacgtcga
     5341 gcggccgttc caggcgctca tggccgccga tcccactcgc tatgcgttgg ccaatcagct
     5401 cgactacttt ctgctgcgcg ccgagcagga gcgggctatc cgcaacggcc cggtcgatgg
     5461 cctcatcgat ggcgggcttg acctggatta ccacgccttc acccaactgt tcacgaccaa
     5521 aggctatttg tccgccgccg aagccgacct gctgcggcgg ctccatgccg ccctgcgcgc
     5581 cgcacttggc ccgcccgacc ttgtgctcta tctgatcgcg ccggcggcgg tggtcgaggc
     5641 ccgctatgcc cgccgcggcc gcccgctgga gatcgcccag cgcgatgatt tggccttgct
     5701 ggagcagttt gtgtccgggt gggtcgccgg catcaccgac gcgccggtca tcaccatcga
     5761 cgcggcggcc gaggactgct attcggctga gcggttggcg gcgctgttgg cacagatgga
     5821 cgaacttgtc gtgaatcagg aatcaggaat tacgaatcag gaattaaaga gggggtaggc
     5881 ctgacaggtg ggcggtcgag gaagcccggt tatgacggat gcttcgttcg cccacctgtc
     5941 aggctttctt agttgacggt gttcagcaca ttgcgccagg ccacgccgcg cagcccgtcg
     6001 gcgatctgct cgaccatctc gatgcccacg ttcttctgcg cttcctgcg
//
LOCUS       DAOMQG010000194         6047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5557_length_6047_cov_9.197597, whole genome shotgun sequence.
ACCESSION   DAOMQG010000194 DAOMQG010000000
VERSION     DAOMQG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_6047_cov_9.197597"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /locus_tag="PLC06_10785"
     CDS             complement(<1..2198)
                     /locus_tag="PLC06_10785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO90972.1"
                     /translation="MKSRGRRIAILLSLLLTAFGLRVHRIGEQRVWWDEGWSVWVARF
                     SPLEILRQTGNDVHPPLYFELLHVWRALSGDSEAGLRLLSAFLGLLTVAMTYALGRRM
                     ARGTLSPPAATLAGVLAALFLTVSRFTIAWSQEIRMYALASLLAVLAVWAARRVWALG
                     RRSDAVVFVLATAGGLYTLYLFAPVWVAINVAWLWTWRAAADRRRAFLHWVGLQLVVP
                     ALFLPWLWYASSGFLSTASATPIRLLDFLHIYWTVLTVGIPVNVAQFNHLTLPAAAVF
                     LAAVGAVVVAVARRRPTAVSRRPTTDDGPRTTDHGRQTTDDRPQTTNHGKQYPFTPSP
                     LPPFSPAPRQDLTLLLAILLIPAIIVYIVSLPRQNFYNPPFNPRYLVIFTPFYSILLA
                     WGLAVLGEWMGKKKGGRGEGGKGARGRAVATTLLLAAFMVAVALVGLWPYYPGRVRVD
                     DFPSLVSTIDAHRQPGDAVVLYTDTDWPVFAYHHPAAWIGVPYQWTITPDMAADFLAP
                     IWESHDAVWLVTTPYSAGGDPQRHVPAWLGERATAVREFTYEDMALTLFARTEERGAS
                     ADRLTQGEPPNALAVPLAGGGTLTGFAQAARDFKSGNTIHLFLYRAAGDEITTQVGLI
                     DGRDVVFAPQTITLPATASAARQQVDLIVPPEAPSGRYRFFIIDADGRVAPFGRANIT
                     QKDATLLTADDVIIANRVDAPFAGGIRLLGYDLAAATARPGESINLRLYWT"
     gene            complement(2198..3916)
                     /locus_tag="PLC06_10790"
     CDS             complement(2198..3916)
                     /locus_tag="PLC06_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRO90973.1"
                     /translation="MPQETLFGLTATVEDRSTYAQLHLQGIRHRRRLSPGSPRAGEQP
                     TASVTVGPGLPVDEIVCLITQPEAAVVPLRRVKTEWDLLLWGYYEVWEAQLPAHPAGT
                     LVRYQIRAHDEDSGEIYWADEGAEYSYLVGDSGPPAWALPAIIYQVFPDRFSPGRGRN
                     WNATSGLSDIYGGTLRGVIDHLDYIAGMGFTAIWLNPFFPDDTHHGYHATDYFAVNPR
                     LGTLDDMRELVDKAHERGIRLLLDFVGNHWGSKHATFQEARANRNSPYYDWYTWKNWP
                     DEYTAYFGVPDLPKLNVDNPAVRDHLLRSIGYWVGEMGFDGLRLDHADGPTYDFWTDV
                     RAVTRSIRPDAWIFGELVRPPDQQLSYAGLFDGTLDFLLCQAMRNTFATGQMGLAAFD
                     AFLNRHETYFPPQSAFSRPSFLDNHDMNRFLWQAGGDKRKLKLAALVQFTLGGAPIVY
                     NGTEVGVTQVRGIHDPNSEGMEECRQPMLWGDAQDTDLSSWFWYLSHLRRDHPALWRG
                     GRETLHMDSAAGTYAYVREDGGEALMVGLNLSDEPREFKVAYTPRGAVATFNLPPVSG
                     DVATFK"
     gene            complement(3958..4344)
                     /locus_tag="PLC06_10795"
     CDS             complement(3958..4344)
                     /locus_tag="PLC06_10795"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HRO90974.1"
                     /translation="MTPSEYLEKITTAIPASAVVIAHRITRHRSSNKDGYFRAILTFT
                     DGSRLEVMEYIRTTAKGEIETNRYSYHWMDADNRLKIRWDNAEHYPKLPGFPHHMHDD
                     DEKNVRPCEPMNFFKVLDTIATSLKA"
     gene            complement(4341..4682)
                     /locus_tag="PLC06_10800"
     CDS             complement(4341..4682)
                     /locus_tag="PLC06_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90975.1"
                     /translation="MQTAGVETKQAMSRRSRLEQLIRLYETGQASDYLDRVLDKAFAQ
                     EAAEEEELISQLRSDLNEFETRYGMDSSTFYKRFRAGEMGDAMDFMEWASLYDMHQSA
                     IERLELLTGQL"
     gene            complement(4703..4939)
                     /locus_tag="PLC06_10805"
     CDS             complement(4703..4939)
                     /locus_tag="PLC06_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90976.1"
                     /translation="MIKVDDIIVQAAAPKAQKEIKESGADLFRFEKRFGMESDDFYNR
                     FEAGEMGNDADFIEWASFIDMYRRAKGHLALLVE"
     gene            complement(5013..>6047)
                     /locus_tag="PLC06_10810"
     CDS             complement(5013..>6047)
                     /locus_tag="PLC06_10810"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HRO90977.1"
                     /translation="ATAYRATQDGWELVATGPATDGRAALDFPEEVSAGVAVGEGGII
                     ELSVPLAVLGNADVGATLTLRALFSEVVSGERAIIDRLPTIGPAALVVPDLGTTQALL
                     DITDPTGDDYGPGTYTYPTDAVFKPGNFDITNFQVGTDDENIVFRFTMAGPVDNPWDG
                     TNGLSLQTFDVYIDTDGGDAGGAAFLPGRNLAAAPGTAWDYAITVEGWESKIFTPGEE
                     GPKEIAGPADFQVITDSGQQKVTIRVPKSVLGDTPEAWRYAAIVMSQEGYPANGVLRV
                     RDVAPKAEQWRIGGAPGGTTNHTRVLDLVWAEAGQQESWLAAFTPSTAPQGSLTAADF
                     ATVEFMTPGQ"
BASE COUNT         1089 a         1856 c         2009 g         1093 t
ORIGIN      
        1 gtccagtaga ggcgcagatt gatgctctcc ccggggcggg ccgtcgccgc ggccaggtcg
       61 tagcccagca gccggatgcc gccggcgaag ggcgcatcga cgcggttggc aatgatcacg
      121 tcgtcggcgg tcagcagcgt ggcgtccttt tgggtgatgt tggcccggcc gaagggggcg
      181 acgcggccgt cggcgtcgat gatgaagaaa cgatagcggc ccgacggcgc ttccggcggc
      241 acgatgagat cgacctgttg gcgggcggcg ctcgccgtcg ccggcagggt gatcgtctgc
      301 ggcgcgaaga cgacatcgcg gccgtcgatg agaccgacct gcgtggtgat ctcgtcgccg
      361 gccgcccgat agagaaacag atggatggtg ttgccgctct tgaagtcacg ggcggcctgg
      421 gcgaagccgg tcaacgtgcc gccgccggcc aggggcacgg cgagcgcgtt gggcggctcg
      481 ccttgggtga gccggtcggc gcttgcgcct cgctcctcgg tgcgggcgaa gagcgtcagg
      541 gccatatcct cgtaggtgaa ctcgcggacg gccgtcgcgc gctcgcccaa ccaggccggg
      601 acgtggcgct gcgggtcgcc ccccgcgcta tagggggttg tgaccagcca aacagcgtcg
      661 tggctctccc agatcggcgc gagaaaatcg gccgccatgt cgggggtgat cgtccattga
      721 tagggaacgc cgatccacgc cgccggatga tggtaggcaa agacgggcca atcggtgtcg
      781 gtatagagca cgaccgcgtc gcccggctgc cgatgagcgt cgatggtgct caccagcgag
      841 ggaaaatcgt ccacccgcac gcgccccgga tagtagggcc acagcccaac cagagcgacg
      901 gcgaccatga aggcggccag aagcaaagta gtggcgactg ctcttccgcg cgcccctttc
      961 cccccttctc cccttccccc cttcttcttc cccatccatt cgccgagcac cgccaacccc
     1021 catgccagca aaatgctata gaacggcgtg aagatgacca gatagcgcgg gttgaagggt
     1081 gggttgtaga agttctgcct gggcagcgat acgatgtaga cgatgatggc gggaatgagg
     1141 aggatcgcca ggaggagggt gaggtcttgc cggggggcgg gggagaaggg gggaagggga
     1201 gaaggggtga agggatactg cttgccgtgg tttgtggttt gtggtctgtc gtccgtggtc
     1261 tgtcgtccgt ggtctgtcgt ccgtggtccg tcgtccgtgg tcggccgacg gctgacagcc
     1321 gtcggccgtc gccgtgcgac cgccaccacg actgccccca ccgccgccag gaacaccgcc
     1381 gccgcgggca gcgtcaggtg gttgaactgg gctacattga ccgggatgcc caccgtcagc
     1441 accgtccagt agatgtggag aaagtccagc aggcggatgg gggtggccga tgccgtggac
     1501 aggaagccgc ttgaggcata ccacagccag ggcaggaaga gcgccggcac aacgagttgc
     1561 agccccaccc aatgcaggaa tgcgcggcga cggtcggccg ccgcgcgcca tgtccacagc
     1621 caggcgacat tgatcgccac ccagaccggc gcgaacaggt aaagcgtgta gaggcccccg
     1681 gccgttgcca gcacaaagac gacggcatcg ctccggcgac ccaatgccca cacccgccgc
     1741 gccgcccaca ccgccagcac ggccagcagt gatgccaggg cgtacatgcg gatttcctgc
     1801 gaccaggcga tggtgaagcg cgaaacggtc agaaacaggg ccgccagcac cccggccaaa
     1861 gtcgcggccg gcggtgatag cgtgccgcgg gccatgcgac ggcccagggc ataggtcatg
     1921 gccaccgtga gcaggccgag gaaggccgac aataaacgga gtccggcctc gctgtcgccg
     1981 ctcagcgcgc gccagacgtg gagcaactcg aagtagagcg gcgggtgaac gtcgttgccc
     2041 gtctgccgca aaatctccag cggcgagaag cgcgccaccc acaccgacca accctcgtcc
     2101 caccacacgc gctgctcgcc gatgcgatgc acgcgcaggc cgaaagccgt caggagcagc
     2161 gagagaagga tcgcgatccg ccggccgcgc gacttcatca cttgaacgtg gcgacatccc
     2221 cactgacagg cggcaggttg aaagtggcga ccgctcctcg cggggtatag gcgaccttga
     2281 attcgcgcgg ctcgtcgctc aggttgaggc cgaccatgag ggcttcgccg ccgtcctcgc
     2341 ggacataggc ataggtaccg gccgcgctat ccatatgcag cgtctcgcgg ccgccgcgcc
     2401 acagggctgg atggtcgcgc cgcagatggc tgaggtacca gaaccaggat gacaaatcgg
     2461 tatcctgcgc gtcgccccag agcatcggct ggcggcactc ttccatgcct tcgctattgg
     2521 gatcatggat accgcgcacc tgtgtcacac ccacttcggt gccgttgtag acgatgggcg
     2581 cgccgcccag cgtaaactgc accagcgcgg ccagcttgag cttgcgcttg tcgccgcccg
     2641 cttgccagag aaagcgattc atatcgtggt tatccaggaa gctggggcgg ctgaatgccg
     2701 attggggcgg gaaataggtc tcgtggcggt tcaggaaggc gtcgaaagcg gcgagaccca
     2761 tctggccggt ggcgaaagtg ttgcgcatgg cctggcacaa caggaagtcg agcgtgccgt
     2821 cgaacagccc ggcatagctg agctgctggt cgggcggccg caccagttcg ccgaatatcc
     2881 acgcgtcggg gcggatgctg cgcgtcacgg cgcggacgtc ggtccagaaa tcatacgtcg
     2941 gcccgtcggc gtggtcgagg cgcaggccgt cgaaccccat ttcgccgacc caatagccga
     3001 tgctgcgcaa caggtggtcg cgcacggcgg ggttatcgac gttgagcttg ggcaggtcgg
     3061 gcacgccgaa gtaggccgta tactcatcgg gccagttctt ccaggtgtac cagtcgtagt
     3121 aggggctgtt gcggttggcg cgcgcttcct ggaaggtggc atgcttgctg ccccaatggt
     3181 tgccgacaaa atcgagcaac aggcggatgc cgcgctcatg ggccttgtcc accaactcgc
     3241 gcatgtcgtc gagcgtcccc aggcgcgggt tcacggcgaa gtagtcggtg gcatggtagc
     3301 cgtggtgggt gtcgtcgggg aagaacgggt tgagccagat ggccgtgaac cccatcccgg
     3361 cgatatagtc gagatgatcg atgacgccgc gcagcgtgcc gccgtagata tccgacaggc
     3421 ctgacgtcgc gttccagttg cggccgcgcc ccggcgagaa gcgatcgggg aagacctggt
     3481 agatgatggc cggcagcgcc caggccggcg gcccggaatc accgaccagg taggagtact
     3541 cggcgccttc gtcggcccaa tagatctcgc cgctgtcttc gtcgtgggcg cgaatctggt
     3601 agcgcaccag cgtcccggca gggtgggcgg gcagttgcgc ttcccacacc tcgtagtagc
     3661 cccacaggag cagatcccat tccgtcttca cacgccgcag cggcacgacg gccgcttcgg
     3721 gctgggtgat caggcagacg atttcgtcca ccggcagccc ggggccgacg gtcacgctcg
     3781 cagtcggctg ttcgccggcc ctgggcgagc cgggcgagag gcgacgacgg tgccggatac
     3841 cttgcaagtg caattgggca taggtgctgc gatcttcaac ggtggccgtg aggccgaata
     3901 gggtttcttg gggcattagg gtactcgtgt tggtatggag acttgcctgg caacggttca
     3961 cgccttgaga gatgtcgcga tggtgtccag cactttgaag aagttcatcg gttcacaagg
     4021 gcgaacgttt ttctcgtcat catcatgcat atgatgtgga aagccgggta gtttgggata
     4081 gtgttcggca ttatcccaac gtatcttaag ccggttgtct gcgtccatcc agtgatagct
     4141 gtaacgattc gtttctattt cgccctttgc tgtggtgcga atatattcca tcacttccag
     4201 tcgactgccg tcggtgaagg tcaaaatagc gcggaagtac ccatccttat tgctcgaacg
     4261 atgtcgagtg atccgatggg cgatcacaac tgcactggcg gggatggcag tcgtgatctt
     4321 ctctaaatat tcagaaggcg tcacaattga cccgtcagca attcaagtcg ttcaatcgca
     4381 ctctggtgca tatcgtaaag agaagcccat tccataaaat ccatagcgtc gcccatttcg
     4441 ccggctctga aacgcttata gaaagttgag gaatccatcc cgtaccgcgt ctcaaactca
     4501 ttcaaatcag agcgtaattg actgatgagt tcttcttctt cggccgcttc ctgggcaaag
     4561 gccttatcca gcacgcgatc cagataatcg ctggcctgtc ccgtctcata gagccggatc
     4621 aactgctcca gacgcgagcg gcggctcatg gcttgcttgg tttctactcc ggctgtctgc
     4681 atgacttatc tccggtggct cgctactcta ctaaaagcgc gagatgcccc tttgcgcgtc
     4741 ggtacatatc gatgaaagat gcccattcga tgaagtccgc atcgttgccc atttcaccag
     4801 cctcgaagcg attgtagaaa tcgtcggact ccattccgaa gcgtttctcg aatctgaaca
     4861 aatcagcacc tgattctttt atttctttct gcgcttttgg tgctgcagct tgcactatta
     4921 tatcgtctac cttaatcatt attatagcgg agacgaggaa aaacggcagg aggcggcttg
     4981 atggcctacc tcctgcccgt aagcgaagat tgtcactgcc ccggcgtcat aaactcaacc
     5041 gtggcgaagt cggccgccgt caggctgccc tggggcgcag tcgatggggt gaaggcggcc
     5101 agccaggatt cctgttggcc cgcttcggcc cagaccaggt cgagcacgcg ggtgtggttg
     5161 gtcgtgccgc ccggcgcgcc gccgatgcgc cattgctcgg ccttgggagc cacgtcacgg
     5221 acgcgcagca cgccgttggc ggggtagcct tcctggctca tgacgatcgc cgcgtagcgc
     5281 cacgcttccg gtgtgtcgcc cagtaccgac ttgggcacgc ggatggtgac cttctgctgg
     5341 ccggagtcgg tgatgacctg gaagtcggcc ggcccggcga tctccttcgg gccttcctcg
     5401 cccggcgtga agatcttcga ttcccagccc tcgacggtga tggcgtagtc ccaggccgtg
     5461 ccgggggccg cggccagatt acgaccaggc aggaacgccg ccccgcccgc gtcgcccccg
     5521 tcggtgtcga tgtagacatc gaacgtttgc agcgacaggc cgttggtgcc gtcccagggg
     5581 ttgtctaccg ggccggccat cgtgaagcgg aagacgatgt tctcatcatc cgtgcccacc
     5641 tggaagttgg tgatgtcgaa gttgcccggc ttgaagacgg catcagtggg gtaggtgtag
     5701 gtgccggggc cgtagtcgtc gccggttggg tcggtgatgt ccagcagagc ctgggtcgtg
     5761 ccgagatcag gcacgaccag cgccgccggg ccgatggtcg gcaggcggtc gatgatcgcc
     5821 cgctcgccgc tgacgacctc gctgaacagc gcgcgcaggg tcagcgttgc gcccacgtcg
     5881 gcgttgccca gtaccgccag cgggacggag agttcgatga tgcccccttc gccgacggct
     5941 acgcccgccg acacctcttc ggggaagtcc agcgccgccc ggccgtcggt cgccgggccg
     6001 gtcgccacga gttcccagcc gtcctgggtg gcgcgatagg cggtcgc
//
LOCUS       DAOMQG010000195         6038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5571_length_6038_cov_10.677921, whole genome shotgun sequence.
ACCESSION   DAOMQG010000195 DAOMQG010000000
VERSION     DAOMQG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_6038_cov_10.677921"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLC06_10815"
     CDS             complement(<1..358)
                     /locus_tag="PLC06_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRO90978.1"
                     /translation="MSNSFQISLTTEIALLGFPRKQPLHGYEIYRRLNETPELRAVWR
                     MKQGRLYALLARLEEEGLLHATHEPQDNRPPRKVFHLTSTGENVFSQWLIEPVKLPRE
                     MRLDFMLKLYFAMWEEP"
     gene            635..1279
                     /locus_tag="PLC06_10820"
     CDS             635..1279
                     /locus_tag="PLC06_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRO90979.1"
                     /translation="MPSTTTSAVTPPPDSTSPLRDDEPANKAEIPEGERAKVTHVFDG
                     DTIEVDLDGRTYRLRYIGVDTPEREEPFYKDAADFNRDLVEDQTVILVRDVSDTDRYG
                     RLLRYVYLEDGTFVNAELMRGGFARLVTFPPDVAQTDYLRGLQTEARDAGVGMWSRPD
                     LTGPCDCDRNLYNCRDFQSRDEAQTCFEYCMDATGRDVHKLDGGGDGLVCEFLP"
     gene            1283..1804
                     /locus_tag="PLC06_10825"
     CDS             1283..1804
                     /locus_tag="PLC06_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90980.1"
                     /translation="MNETMYPPLIYFFGLPAAGKNYAGRVMSEEFGYTFYDGDLDLTL
                     EMRDAIREERPFTEAMRDRFYTLLIERIAELRAGHPHLAFCQATFKEQHRDMIADAFP
                     DVVFVLVEATPEVRASRLARGGNPVTVDYAQRIGTFFEPPRHPHFVITNNDGREDIVR
                     QLIALLNWFAGED"
     gene            complement(1801..2277)
                     /locus_tag="PLC06_10830"
     CDS             complement(1801..2277)
                     /locus_tag="PLC06_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRO90981.1"
                     /translation="MGEPLTPVGRELYDRSIPFREFRHPGVVTSLEQAAEERGQRPGQ
                     IVRSILFRVTQDEYLMVLVAGPRQVDWKALRHYVGQSRLTMASADEVREVTGYEIGAV
                     SPFALARPVRVMLDESVTREEEISIGSGVRSTTVILKSADLMRALGGVERVSLTHD"
     gene            complement(2286..3035)
                     /locus_tag="PLC06_10835"
     CDS             complement(2286..3035)
                     /locus_tag="PLC06_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRO90982.1"
                     /translation="MTTNHTESRPIIGLTTYRKMVDKNNPYPYMALMATYVEAVTAAG
                     GIPLLIPLGLDEDALRVLLSQVDGLVLTGGGDIAGERYRSEHEDFIFNVDADRDRVEF
                     FLAREAMAQDKPMLAICRGHQVLNVALGGTLYEDVKAWMPGAIKHDYFEVFPREHKAH
                     AVAIEPGSKLAEALGRRETQVNSLHHQGIRDLAPGLAAVAHAPDGLIESVEGVDHPFA
                     LGVQWHPENLVHDDPMMLSLFRGLVEAASAN"
     assembly_gap    3084..3183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3319..3684)
                     /gene="rplS"
                     /locus_tag="PLC06_10840"
     CDS             complement(3319..3684)
                     /gene="rplS"
                     /locus_tag="PLC06_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRO90983.1"
                     /translation="MSDLLLKAFDKEINSDLPAVNVGDSVTVYVRINVGDKNERVQMV
                     RGTIIRVRNAGNNANFTVRRIASNGIGVERTFLASSPRIEKIEIHSQSHVRRARLYYL
                     RNRTGKATRLRAKRAFEQR"
     regulatory      4025..4127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4341..4733
                     /gene="gcvH"
                     /locus_tag="PLC06_10845"
     CDS             4341..4733
                     /gene="gcvH"
                     /locus_tag="PLC06_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRO90984.1"
                     /translation="MSTKIMPDLRYAKSDEWIRVEGDEAVVGISDYAQDALSDIVYVE
                     LPSVGDTINAGNPFGVVESVKAASDVLLPVDGEIIAVNDAAVDSPESLNGDPYGTWLV
                     RLRMTNPSQVDALMDAAAYEAYNATREH"
     gene            4936..>6038
                     /locus_tag="PLC06_10850"
     CDS             4936..>6038
                     /locus_tag="PLC06_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRO90985.1"
                     /translation="MPADRFVKRHIGPRSHDVTAMLRDLGLDSLDELIDQAIPASIRM
                     DGYLAMDPPRSEQAVLDELRRIAGKNKVYRSFIGMGYYGTITPPVVQRNVLENPGWYT
                     AYTPYQPEISQGRLEALINFQTMVTDLTGLEIANSSLLDEATAAAEAMTLCHRALPRN
                     SQANVFFVSDQVHPQTIAVVRTRAEPFGFKVVVGDHATYDFAEATFGVLVQYPATNGD
                     VYDYRDFAAKAHANGALVVAATDLLSLVLLTPPGEWGADVALGNSQRFGVPMGYGGPH
                     AAFFATKDEYKRIMPGRLVGVTIDATGKKGYRLTLTTREQHIRREKATSNICTAQVLL
                     AVMASMYAVYHGPVGLRRIATRVHLLTEVMAAGL"
BASE COUNT         1276 a         1859 c         1641 g         1162 t
ORIGIN      
        1 agggttcttc ccacatcgcg aaatacaact tcagcatgaa gtccagtcgc atttccctcg
       61 gtaatttgac aggctcgatc aaccactggc tgaagacgtt ttcacctgtt gacgtcagat
      121 ggaaaacctt gcggggcggc cgattgtctt gcggctcatg cgtagcatgc agcagcccct
      181 cctcctccaa ccgcgccagc aacgcataga gtcggccctg cttcatgcgc cataccgccc
      241 taagctccgg cgtctcattc aggcgacgat agatctcgta accatgaagc ggctgcttgc
      301 gtgggaagcc cagcaaggcg atttcagttg taagagaaat ctgaaatgag ttactcatag
      361 cttgagtatt cattcggcaa ttatacagag catgggtagc cagtcaatct tctttcagat
      421 gacagacgaa caaaaacagc tactattcac ggatcgagga attaatctat gatcggacat
      481 agaatcgtcg agggaacctt ccgagagcgt caggcccttg ccgttctgca caagtcatta
      541 accatataaa gagccttcat ctttcatgat tcgatccaca catcttttct atttattact
      601 gtgcattgcg ggctgtctgg ttctgactgc ttgcatgccg tcaacaacaa cgtccgccgt
      661 caccccgccg ccggattcaa cttcccccct acgtgatgac gaaccggcca acaaagccga
      721 aataccagag ggcgaacgcg ccaaggtcac tcatgtattc gatggcgaca ccatcgaggt
      781 ggatctggac ggccgcacct atcgcctgcg ctatatcggc gtcgatacgc cggagcgcga
      841 agaaccattc tataaggatg cggccgactt caatcgtgat ctggtggaag accagacggt
      901 gatcctcgtg cgagatgtca gcgatacgga ccgttatggc cgcctgctgc gttatgtcta
      961 cctggaggat ggcacattcg tcaacgcgga gctgatgcgt ggcggttttg cgcggttggt
     1021 cacctttccg ccggacgtgg cccagaccga ctatctacgc gggctacaaa ccgaagccag
     1081 ggacgccggc gtgggcatgt ggagtcgccc cgacctcacc ggcccctgcg attgcgatcg
     1141 aaatctctat aactgccgtg actttcaatc gcgcgatgag gcgcaaactt gttttgaata
     1201 ttgcatggac gccaccggcc gcgacgtgca caaactggat ggtggcggag acgggctggt
     1261 gtgcgagttt cttccctgag cgatgaacga aacgatgtac ccgccgctca tctacttctt
     1321 cgggctgccg gcggccggca agaactacgc gggacgtgtg atgtccgaag agttcggcta
     1381 cacattttat gacggcgacc ttgacttgac cctggagatg cgggatgcca tacgcgagga
     1441 gcggccattc accgaagcaa tgcgcgatcg cttctacaca ttgctcatcg aacggatcgc
     1501 cgaattgcgg gccgggcatc ctcacctggc tttctgtcag gccaccttca aggaacaaca
     1561 ccgcgacatg atcgccgatg cgtttcccga tgtagtcttc gtgctggtgg aggcgacacc
     1621 tgaagtgcgc gcaagccgcc tggcgcgagg cggcaacccg gtcaccgttg attatgccca
     1681 acgtatcggc acgttctttg agccaccacg ccatccccat ttcgtcatta ccaacaacga
     1741 cggccgcgaa gacatcgtgc gccaactgat cgcgctgctc aactggtttg ccggagagga
     1801 ctaatcatgg gtcagcgaca cgcgttcgac accaccgagc gcgcgcatca ggtcggccga
     1861 tttcaggatg acggtggtgg aacgcacgcc tgagccgatc gagatttctt cttcccgggt
     1921 cacgctctcg tcgagcatca cccgcaccgg tcgagccaac gcaaacggcg acaccgcccc
     1981 aatctcatag ccggtcacct cgcgaacctc gtcggccgag gccatcgtca accgtgattg
     2041 gcccacgtaa tggcgcagcg ccttccaatc cacctggcgc ggccccgcga caagcaccat
     2101 caaatactcg tcctgcgtca cccggaacag aatgctgcgg acgatctgcc ccggtcgttg
     2161 gccgcgctcc tcggccgcct gctccagaga ggtgaccact ccggggtggc ggaattcgcg
     2221 aaacggaata cttcggtcgt agagttcgcg gcctaccggg gtcagtggct cgcccattgg
     2281 ggtcatcagt tggcgcttgc ggcctcaacc agaccgcgga acagtgacag catcattgga
     2341 tcgtcatgaa ccagattctc gggatgccat tggacgccca gagcgaaagg atggtcaacg
     2401 ccctcgacgc tctcgattag cccatcgggc gcgtgggcca cggccgccag cccgggggcc
     2461 aggtcgcgga tgccctgatg gtgaaggctg ttcacctgag tttcgcggcg gccaagcgcc
     2521 tcagccagct tgctgcccgg ctcgatagca acggcatggg ccttgtgttc gcgcgggaaa
     2581 acctcaaagt agtcatgttt aatcgcgccc ggcatccagg ccttcacatc ttcgtagagc
     2641 gtgcccccaa gggccacatt cagaacctgg tggccgcgac agatggccag catgggcttg
     2701 tcctgggcca tcgcctcgcg cgccagaaag aactctaccc ggtcgcggtc ggcgtcgaca
     2761 ttgaagataa aatcctcatg ttcgctccgg taacgctccc cggcaatatc gccaccgccc
     2821 gtcaacacca ggccatccac ttgcgacagc agcacgcgca gggcatcttc atccagtccc
     2881 aacgggatga gcaaagggat gcccccggca gcggtcaccg cctccacgta ggtcgccatc
     2941 agcgccatat aggggtaagg gttgttcttg tctaccatct ttcgataggt ggtcaggccg
     3001 ataatcggcc tcgattcagt gtgattcgtg gtcataagaa tagtgtagct ttcgattcgc
     3061 gagcttgcca aaataaaaaa ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnntggtcat aagaatagtg tagctttcga ttcgcgagct tgccaaaata aaaaaggtga
     3241 aagatcgctc tttcaccctg tattttccaa catttcgtgc tggaagtcat ccaacacgcc
     3301 gccaacgacg cggattcttt agcgctgctc aaaagcacgc ttggcgcgaa gacgggtcgc
     3361 cttgccggtg cggttacgca ggtaatagag acgtgcgcgg cgcacatggc tctggctgtg
     3421 aatctcgatc ttctcgatgc ggggcgagct ggccaggaag gtacgctcaa cgccgatacc
     3481 gttcgaagca atgcgacgaa cggtaaaatt ggcgttgttg ccggcgttgc gcacgcggat
     3541 aatcgtgccg cggaccatct ggacgcgctc attcttgtca ccgacattga tgcgcacgta
     3601 aaccgtcaca ctatcgccaa cattcaccgc cggcaggtcg gagttaattt ccttatcaaa
     3661 agctttcagc agtaagtcag acatgatcat tctccaaaga ccggaaaaaa caacgttcct
     3721 gccggaacgt cgtctctaca ttcccgttaa cccgggtatt agtcacgtaa aatagaagcc
     3781 caactgggtg ggccgacttc aaactatagc atgccacggt taatttggca atcccagttt
     3841 gctttttggg tcgcgatcaa ttttggcgtg cgtcaggggc cgcgcgcctt tccaaggcgc
     3901 gatatcccag gctgatctta taccaaatct gaacaggccc caaacacaaa actcaatgat
     3961 gcttgacgca tcccccccac cgactacaat atatgcatca gttgttgcta acgaaatagg
     4021 atgcagcggc cgcccgggag agtaccccgc gcggggtcgc cgaaggggca aggaggcggg
     4081 cggaatcgcc tttgaatctc tcaggcacaa gggaccgagt ggccgggttt cttctggaaa
     4141 gcgtcgagca cgtcttcgtg ctcccaccga aggggcaagc gggtatgagc cacacgcgca
     4201 taactccgtg aatctctcag gtttatgaca gaggacgccg gcgagaacgc ctcaggcagg
     4261 cattctcgcc cggcgtcctc gtttatcttc atctgggcca accaaatctc gatcatcagt
     4321 aacttaaagg agaaatgacc atgagcacga aaatcatgcc cgatctgcgc tacgccaaga
     4381 gcgatgaatg gattcgcgtg gaaggggacg aggcagtagt cggtatcagc gactatgcgc
     4441 aagacgccct gtccgacatc gtctatgtgg aactgccgag cgtaggtgac acgattaacg
     4501 cgggcaaccc gttcggcgtc gtcgagtcgg tcaaggcggc gtccgacgtg ctgctccccg
     4561 tcgatggcga aatcatcgct gtcaatgacg cggccgtcga ttcacccgaa tcccttaacg
     4621 gcgaccccta cggtacctgg ctcgttcgtc tgcgcatgac caacccgtcc caggtcgacg
     4681 cattgatgga cgccgccgct tacgaagctt acaacgcgac gcgcgaacac taacaaacta
     4741 cgaacgacga gcgacgaact acgaataaag aatccggagc gccggatcgt cgttcgtgga
     4801 tcgtagttca tcgtttgtcg gaggaattat cccatgacat cacccactcc ccgcgccaac
     4861 tggcaggata tcatcgaagc tcctgactcg gccgccggca acggcgcgcc ccaactcagc
     4921 aaccgcgacc tgctcatgcc cgccgaccgc ttcgtgaagc gccacatcgg cccgcgcagc
     4981 cacgacgtca ccgccatgct ccgcgacctc ggcctcgact cactggatga gttgatcgac
     5041 caagccatcc ccgcgtccat ccgcatggat ggctatctgg ccatggaccc gccgcgtagc
     5101 gaacaggccg tcctcgatga gctgcgccgc atcgccggca agaacaaggt ctatcgctcc
     5161 ttcatcggca tgggctacta cggcacaatc accccgccgg tcgtccagcg caacgtgctg
     5221 gagaaccccg gctggtatac ggcctacaca ccctatcagc cggagatctc gcagggccgc
     5281 ctcgaagcgc tcatcaattt ccagacaatg gtcacagacc tgaccggcct ggagatcgcc
     5341 aactcatcgc tgctggacga ggccacggcc gcggccgaag cgatgaccct gtgccatcgc
     5401 gccctgccgc gcaactccca ggccaacgtc ttctttgtct ccgatcaggt acatccgcag
     5461 accatcgccg tcgtacgcac ccgcgccgaa ccgttcggat tcaaggtcgt cgtcggcgac
     5521 cacgccacct acgacttcgc cgaggcgacc ttcggcgtgc tggtgcaata tccggccacc
     5581 aacggcgacg tctacgacta tcgcgatttc gcggcgaagg cccatgccaa cggggcactc
     5641 gtcgtcgccg ccaccgacct gctgtcgctg gtactgctga cgccccccgg cgagtggggc
     5701 gcggatgtgg cattgggcaa cagccagcgc ttcggcgtgc cgatgggcta tggcggccca
     5761 cacgcagcct tcttcgccac caaggacgaa tacaagcgca tcatgcccgg ccgcctcgtg
     5821 ggcgtcacca tcgacgccac cggcaagaag ggctatcgcc tgacattgac cacccgcgag
     5881 cagcacatcc gccgcgagaa ggcgaccagc aacatctgca cggcgcaggt gcttctggcc
     5941 gtcatggcct cgatgtacgc cgtttatcac gggccggtcg ggctacggcg catcgccacc
     6001 cgcgttcacc tgctgaccga ggtcatggcc gccgggct
//
LOCUS       DAOMQG010000196         6008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5595_length_6008_cov_7.403830, whole genome shotgun sequence.
ACCESSION   DAOMQG010000196 DAOMQG010000000
VERSION     DAOMQG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_6008_cov_7.403830"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PLC06_10855"
     CDS             complement(<1..806)
                     /locus_tag="PLC06_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90986.1"
                     /translation="MTQITATTSTSESLGGRAMHWLRTINPVYYIALVLLVAVGYLNP
                     VFYTPDPFLAFLARAAPLMILTAGQVFVLVSGGFDLSVGSVVTSTVIISAILANNDPN
                     STWWVILFLLAGGVLVGLINGAVTTFLKVPSLISTLGMLLVVRGVGLLWSGGAPRGYL
                     PDNLRMFGRSGIEGIFGLRQFPYAVILLVVFAVLYWLLLHRMNYGRQLLALGDNPRAS
                     RLSGVNISLVRVSAFVICSVSAAIAGILIGGRGGVSIEAGTGLEMQSIAAA"
     gene            complement(903..1922)
                     /locus_tag="PLC06_10860"
     CDS             complement(903..1922)
                     /locus_tag="PLC06_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO90987.1"
                     /translation="MTALTPSAASPSVIDRARAWARARNIPPVYGILVIIFFVSILAD
                     LFFGIDNQLFIFNPTILTNILVRSVALGIVAVGQTFVVLGASIDLSVAYMISVTAVGA
                     SYLMAGDPNRVWWTVIVLFVVGAVVGLVNGTIITKLKVNPLIATLGVGLILKGLLNAS
                     FANFAGSVPISFQAFAYNAIGPLPISVLVLFAVVIAGWFLLRRTRYGAHLYAVGGSEE
                     TARLSGLHTDRILTIAHVICSMTAVLTGLFVVSRLRSGAPWVGTDGLYDLESIAATVI
                     GGTALSGGKGGVWGTLAGVLIFSLLDTMFNQIGIDAYMKQILRGAVIILAVGFYTFRS
                     KAEQG"
     gene            complement(1967..3586)
                     /locus_tag="PLC06_10865"
     CDS             complement(1967..3586)
                     /locus_tag="PLC06_10865"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRO90988.1"
                     /translation="MAENFLTETEHEPIAPPPAPPAAQPVLEVAHIAKQFPGVLALDD
                     VSVAFYPGEVHAVVGENGAGKSTLMKVLAGAIRPDSGELRFDGRPVTFNHPAEAQQAG
                     ISIIYQEFNLLPERTVAENIFLGREPHRFGVIDYKQLEADTLAVLERLEAQDLISPTA
                     QLGTLSVAQQQIVEIAKALSFDAKVLIMDEPTAALASQEVAVLTDLVRRLQAGGMAII
                     YISHRLVEVFDLAQRVTVLKDGQKVTTEMIGDLNMSRIVELMVGRVLSEYFPERARPE
                     DIGEVVLRLRGASNDALHDIDLELRAGEIIGIAGLQGSGRTALAQAIFGVEPFETGTM
                     EFAGRAELFATPSQAIRRRLGYVTEDRKYEGLALKQPINDNILLTLRTLQNKVSRVFR
                     NGTRSDREMALELARRVDVRAPTMEREVRTLSGGNQQKVVLAKWLATDAKLLIFDEPT
                     RGIDVEAKASIHERIRELARAGTAVLMISSELPEVIGMSDRILVMWDGAIIGELPAGA
                     SEAEIMHLATGQKEDTKYEIRDTSEERALDS"
     gene            complement(3741..4643)
                     /locus_tag="PLC06_10870"
     CDS             complement(3741..4643)
                     /locus_tag="PLC06_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRO90989.1"
                     /translation="MKFGVNTFNFVSPFRTAADLSLLDRVKGMGFDLIEIAFEDPAAI
                     DVAALADHAAALDLGVLACGAFGPGRNLASADAAERRATAGYIRGLIDAAALLAPTLG
                     GMIPVVGGPMYGAVGKTPAADRDARQRERDLAAAELRPLAAYAGDHGVRLAMETLNRF
                     ETDLLNVVEQGMAMVEAIGSPHVGLHLDTFHMHLEERNSGDAIRLAGDRLYHFHACEN
                     DRGVPGRGQVRWPSVAVALDDIGYGRDGRDGRDGRDGFVVIESFTPSVTSIARAVCIW
                     RDVAPDQDTIAREGLGFLRKLLAR"
     gene            complement(4833..5990)
                     /locus_tag="PLC06_10875"
     CDS             complement(4833..5990)
                     /locus_tag="PLC06_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRO90990.1"
                     /translation="MKSLNVAMIGTKFMGKAHSNAWSNAPRFFDMGIKPVLKVIVGTD
                     PAGTKAMADTWGWEEWSTDWREVVARPDIDIVDIATPTWLHHDMAIAAAEHGKHIFLE
                     KPFSLTVEQAREMLAAAEKAGVVHYLNHNYRRVPAVRLAKRLISDGFVGRIFHWRSAY
                     LQDWIVDPDFPLTWHLRAEMAGYGAHGDLGSHSVDLARYLLGDISAVTGMTATFIPTR
                     PLPGAGAATFTAGTGESSESGPVTVDDASFFIAEFANGALGSFEVSRFAPGRKNYNYF
                     EIYGSEGSILFNLERMNELQVFSRHDPAHAQGFRTILATEAGQHDYIANWWPPGHIIG
                     YEHEFHHAVVDFMKAIETGRTISPNFYDGLKEMEVLAAAVESARDGCRVSL"
BASE COUNT         1104 a         1944 c         1925 g         1035 t
ORIGIN      
        1 gccgcggcga tggattgcat ctccagcccc gtgccggcct caatcgacac gccgccgcgg
       61 ccgccgatca ggatgccggc gatggccgcc gacaccgagc agatgacgaa ggccgaaacg
      121 cgcaccaggc tgatgttgac ccccgacagg cgcgaggccc gcgggttgtc gcccagcgcc
      181 agcagctggc ggccgtaatt catgcggtgg agcagcagcc agtagaggac ggcaaacacc
      241 accagcagga tcaccgcgta ggggaattgg cgcaggccga agatgccttc gatgccgctg
      301 cggccgaaca tgcgcaggtt gtcgggcaga tagccgcgcg gcgcgccgcc cgaccacagc
      361 agccccacgc cgcgcacgac gagcagcatg cccagcgtcg agatgagcga cggcaccttc
      421 aggaaggtcg tcaccgcgcc gttgatcaga cccacaagca cgccgcccgc cagcaggaac
      481 aggatgaccc accaggtcga attggggtcg ttgttggcca ggatggcgct gatgatgacg
      541 gtcgaggtga cgaccgagcc gaccgacagg tcgaacccgc cggagacgag cacgaacacc
      601 tgccccgcgg tcaggatcat caatggtgcg gcgcgcgcca gaaaggcgag aaagggatcg
      661 ggcgtataga acaccgggtt caggtagccg acggccacca gcagcaccag cgcgatgtaa
      721 tagaccgggt ttatcgtccg cagccagtgc atcgcccgcc cgcccaacga ttccgaagtc
      781 gatgtcgtcg ctgtaatctg tgtcataaca tccttcctcc agttacgaaa tacgagatac
      841 gaaatacgag ttcagaaccg ttcttcattc gtaattcgta attcgtaatt cgtaattcgt
      901 aattacccct gttccgcctt cgaccggaag gtatagaacc cgacggccag aataatgacc
      961 gcgccgcgca ggatttgctt catgtaggcg tcgatgccga tctggttgaa catcgtgtcc
     1021 agcaggctga agatgagcac gccggccagc gtgccccaca cgccgccctt gccgcccgac
     1081 agcgccgtgc cgccgatgac cgtggcggcg atcgactcca ggtcatagag tccgtcggtg
     1141 cccacccagg gcgcgccgga gcgcagacgg ctgacgacga acaacccggt cagcacggcc
     1201 gtcatgctgc atatcacgtg agcgatagtc agaatacgat cagtgtgcag acccgacagg
     1261 cgggccgtct cctcgctgcc gcccacggcg tagagatgcg ccccgtagcg cgtgcggcgc
     1321 agcaggaacc agccggcgat gaccaccgcg aacagcacca gcaccgagat gggcagcggg
     1381 ccgatggcgt tataggcgaa ggcctgaaag ctgatcggca ccgacccggc gaagttggcg
     1441 aaggacgcat tgagcagccc tttgaggatc agccccacgc ccagcgtggc gatgagcggg
     1501 ttcaccttta gtttggtgat gatcgtgccg ttgaccaggc cgacgaccgc gccgacgacg
     1561 aacagcacga tgaccgtcca ccacacgcga ttggggtctc cggccatgag ataggacgcg
     1621 ccgacggccg tcacgctgat catgtaggcc accgacaggt caatcgacgc gccgagcacc
     1681 acgaacgtct gccccacggc gacaatgccc agcgccaccg accgcaccag gatgttggtg
     1741 aggatggtcg ggttgaaaat aaacagctgg ttgtcgatgc cgaagaacag atcggccagg
     1801 atcgacacaa aaaatatgat gaccaggatg ccgtagaccg gcggaatatt gcgcgcgcgc
     1861 gcccacgccc gcgcccggtc gatgaccgat ggggatgctg ccgatggagt tagtgccgtc
     1921 ataacaatct ccaaacaatt acgaattacg aattacgaat tacgaattac gaatcgagag
     1981 cgcgttcttc actcgtatct cgtatctcgt atttcgtatc ttccttttgc cctgtcgcca
     2041 gatgcatgat ctccgcctcg cttgcgccgg cggggagttc gccgatgatg gccccgtccc
     2101 acatgacgag gatgcggtcg ctcatgccga tgacttcggg cagttccgag gagatcatca
     2161 gcacggccgt gcccgcgcgg gccagctcgc ggatgcgctc atggatgctg gctttggctt
     2221 ccacgtcgat gccgcgcgtc ggctcgtcga aaatgagcag cttcgcgtcg gtggccagcc
     2281 atttggccag caccaccttc tgctgattgc cgccgctcag cgtgcgcacc tcgcgctcca
     2341 tcgtcggcgc gcgcacgtcc acacgccggg ccagttccag cgccatctcg cggtcgctgc
     2401 gtgtcccgtt ccggaagacg cggctcacct tattctgcaa cgtgcgcaat gtcagcagga
     2461 tgttgtcgtt gatgggctgc ttcagcgcca gcccctcata cttgcggtct tcggtcacat
     2521 agccgaggcg gcggcgaatc gcctgggacg gggtggcgaa cagctcggcc cgcccggcga
     2581 actccatcgt gcccgtctcg aatggctcga cgccgaagat ggcctgggcc agcgccgtgc
     2641 ggcccgaccc ctgcaacccg gctatgccga tgatctcccc ggcgcgcagc tccaggtcga
     2701 tgtcgtggag cgcgtcgttc gacgccccgc gcagccgcaa gaccacctcg ccgatgtctt
     2761 cgggccgggc gcgttcgggg aaatattccg acagcacgcg gccgaccatc agctccacga
     2821 tgcggctcat gttcagatcg ccgatcatct ccgtggtcac cttttggccg tccttcagca
     2881 cggtcacgcg ctgggccagg tcgaacacct ccaccagccg gtgagagata tagatgatgg
     2941 ccattccgcc ggcttgcagg cggcgcacca gatcggtcag cacggccact tcctgcgagg
     3001 ccagcgcggc cgtcggctcg tccatgatca gcaccttggc gtcgaagctg agcgccttgg
     3061 caatctcgac tatttgctgc tgggccaccg acaacgtgcc cagctgggcg gtggggctga
     3121 tcaggtcctg ggcctccagc cgctccagca cggcgagggt atcggcctcc agctgcttgt
     3181 agtcgatgac gccgaagcgg tggggttcgc ggccgaggaa gatgttctcc gccaccgtgc
     3241 gctccggcag caggttgaac tcctgataga tgatgctgat gcccgcttgc tgggcctcgg
     3301 ccgggtggtt gaacgtcacc gggcggccgt cgaaccgcag ttcgccgctg tcgggccgga
     3361 tggccccggc cagcaccttc atcagcgtgc tcttgcccgc gccgttctcg cccaccacgg
     3421 cgtggacttc gccgggatag aaggcgaccg acacgtcatc gagggccagc acgccgggga
     3481 actgcttggc gatgtgggcc acttccagca cgggctgcgc ggccggcggt gccggtggcg
     3541 gcgctattgg ttcatgctcg gtttcagtca aaaaattttc ggccataaaa ttaccccgaa
     3601 attattataa attgcgaatg aagcgcggga tgaatcccgc ggctgctacg caaaaaccgg
     3661 ctgaagccgg ttgccggcgc atcctcaacc ggtttcaacc gggttttgtg tgccggcggc
     3721 ggatttcaat ccgccggttc ctaccgcgcc aacagcttcc tcaaaaaccc cagcccctcg
     3781 cgggcgatgg tgtcctgatc gggggcgacg tcgcgccaga tgcagacggc gcgggcgatg
     3841 gacgtgaccg agggcgtgaa gctctcgatg acgacaaacc cgtcgcggcc gtcgcggccg
     3901 tcgcggccgt cgcggccata gccgatgtcg tccagggcga cagccaccga cggccagcgc
     3961 acctgccccc ggccgggcac gccgcggtcg ttctcgcagg cgtggaaatg atagagccgg
     4021 tcgccggcca gccggatagc gtcgccggag ttgcgctctt ccaggtgcat gtggaacgtg
     4081 tccagatgca agcccacatg cgggctgccg atcgcctcga ccatggccat gccctgctcc
     4141 acgacattga gcaaatccgt ctcgaagcga ttgagcgttt ccatggccag ccgcacgccg
     4201 tggtcgccgg cataggcggc cagagggcgc agctcggccg cggccaggtc gcgctcgcgt
     4261 tggcgggcgt cgcggtcggc ggcgggggtc ttgcccaccg cgccgtacat cgggccgccg
     4321 acgacgggga tcatcccgcc cagcgtcggg gccagcagcg cggcagcgtc gatgaggccg
     4381 cggatatagc cggccgtggc gcggcgctcg gcggcgtcgg ccgacgccag attgcgcccc
     4441 ggcccaaacg cgccgcaggc cagcacgccc aggtcgagcg cggccgcatg atccgccagc
     4501 gcggccacgt cgatggccgc cgggtcctcg aaggcgatct cgatgaggtc aaagcccatg
     4561 cccttcacgc gatccagcag cgacagatcg gccgccgtgc gaaagggaga gacgaagtta
     4621 aacgtattga cgccaaattt catggttttt cctgggagag aatccacaga tttcacagat
     4681 tacacagatt tagccgactg tttcaaaagc actttcactt gcagccagca acttcagttg
     4741 cgatcttcgg cgctaaagcg ccggattacg agtgggtgaa tggtcttttc ttgcaatctg
     4801 tgaaatctgt gaaatctgtg gattctcctc tcctataaac tcaccctaca accatcacga
     4861 gccgactcga cggccgcggc caggacttcc atctctttca gcccgtcgta aaagttgggc
     4921 gagatcgtcc ggccggtctc gatggccttc atgaaatcga ccaccgcgtg atggaattcg
     4981 tgctcatagc cgatgatgtg gccggggggc caccagttgg cgatgtagtc gtgctgcccg
     5041 gcctcggtgg ccaggatggt gcggaacccc tgcgcatggg ccgggtcgtg gcggctgaac
     5101 acctgcagct cgttcatccg ctccaggttg aagaggatgc tgccctcgct gccgtagatc
     5161 tcgaaatagt tgtagttctt gcggccgggg gcgaagcgcg acacctcgaa cgaccccagc
     5221 gcgccgttgg cgaactcggc gatgaagaac gaggcgtcat cgacggtgac ggggccgctc
     5281 tcgctcgatt cgcccgtccc ggcggtgaag gtggccgcgc ccgcgccggg cagcggccgc
     5341 gtggggatga aggtggcggt catgcccgtc acggcgctga tgtcgcccag cagatagcgg
     5401 gccaggtcga cgctgtggga gccgagatcg ccgtgcgccc catagccggc catctccgcc
     5461 cgcaggtgcc aggtgagcgg gaagtcggga tcgacgatcc agtcctgcag ataggcgctg
     5521 cgccaatgga agatgcggcc gacgaagccg tcgctgatga gccgcttggc cagccgcacg
     5581 gcgggcacgc ggcggtagtt gtgattgagg tagtggacga cgcctgcctt ctcggccgcg
     5641 gccagcatct cccgcgcctg ctcgacggtg agcgagaacg gcttctccag gaagatgtgc
     5701 ttgccgtgtt cggccgcggc gatggccatg tcgtggtgga gccaggtcgg cgtggcgatg
     5761 tcgacgatgt cgatgtcggg ccgggccacg acctcgcgcc agtcggtcga ccattcttcc
     5821 caaccccagg tgtcggccat cgctttcgtg ccggcggggt cggtgcccac gatcaccttc
     5881 agcacgggct tgatgcccat gtcgaagaag cgcggcgcgt tggaccaggc gttgctgtgg
     5941 gccttgccca taaatttcgt gccgatcatg gcgacattca gactcttcat ttcaatatcg
     6001 tccccttg
//
LOCUS       DAOMQG010000197         6002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5602_length_6002_cov_7.661342, whole genome shotgun sequence.
ACCESSION   DAOMQG010000197 DAOMQG010000000
VERSION     DAOMQG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5602_length_6002_cov_7.661342"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2671)
                     /locus_tag="PLC06_10880"
     CDS             complement(<1..2671)
                     /locus_tag="PLC06_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRO90991.1"
                     /translation="MATSNKSDKTSTTRQTGAKRQASATSKAKSNSSSKPAAKNPAAK
                     KSTAAKKAPPPRLDDGQRSLLAGIVILFLTAILTLSLLSPNQGQFTQWLSGLAGLAFG
                     IGRVVIPLITGGIGVFLVLRGMEQEPELPWFRLLGAAVVFLVFLAFASMIAYQREQAY
                     ESLYAVAAAGKGGGYLGSLLATALARLLGDLGTIATLMVVGLAGVVLAAGLSRDQVAG
                     WFEREPRPSTGAEPAERTVRINPGREAAANAIPVARPPTADRRPEPQLRLIPEEGEAP
                     ARGAAVSVHEKPKRGKKETPPPVFIEADRPAAAVVPGSIWTLPRVSDVLESGTDIDVN
                     SASIRERVEVIEQTLESFGAPATVVEINQGPTITQFGVEPNYVVMRGGKRTKVKVGKI
                     AGLADDLALALAARSIRIQAPVPGKGYVGIEVPNSAKALVSLRDVMESAEYGRMKSPL
                     RVGLGQNVAGQPIAADLTAMPHVLIAGTTGSGKSVCVNGIIACLLLQNTPDDLRLVMV
                     DPKRVELTGYNGIPHLAAPVVVDMDRVIGTLQWALREMDNRYKLFAGAGARNLVEYNK
                     KIRRDATVQKLPYIVIVIDELADLMMLAPEDTERSITRLAQMARATGIHMVIATQRPS
                     VDVVTGLIKANFPARIAFAVASSTDSRVILDATGAERLLGQGDMLFQSPDAAAPVRLQ
                     GCFVSDGELARLISYWQTARRAQVIAAPGATAGAAPVERPATDDGRRTSTDHGPQTMP
                     TQARPVKEPVVIIDPQPGPPAYVPPPISHLNAPVTDTTTPAPQRRAPKIPMGPPDEVR
                     STNDERTSAHRKPVMDDEVTDLEEDADEVIASGVGRATGVASAGGHLDDVLQQPLWEA
                     LQQMENDNGNVAEGDDELLPEAIALVRSLNKAST"
     gene            complement(2798..3577)
                     /locus_tag="PLC06_10885"
     CDS             complement(2798..3577)
                     /locus_tag="PLC06_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90992.1"
                     /translation="MRNLLRFRLGWAALLLSIATMLVAGTGVVLAEDDAQGKRAYMPA
                     VMFQRPPAMDCNVPGASFTTLSIAGPPLTVDPETNRDLNLGVRGYIRINAALQLKNLG
                     PVHDAKAPQLWGMFGDKRTPVFTSAYNADRGPNPPWEVTLLGMGTKPGEVIHTPDSGY
                     DIGGGYEYLVLYAAPTRVTLHIGREDELFGYVVHIEDVCVDPDLVALYRQSHAAGRHS
                     LPALRGHAPFGRASGNEVKAAVRDWGLFLDARSRNDWWQGR"
     gene            3807..4829
                     /gene="trpD"
                     /locus_tag="PLC06_10890"
     CDS             3807..4829
                     /gene="trpD"
                     /locus_tag="PLC06_10890"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRO90993.1"
                     /translation="MPLRTAIAKVINRQDLSFEEAAAAMDAIMGGAATPAQIGSYLTA
                     LRMKRETADEIAGSAFSMRRHVIPVEVPLSGDEFLLDTCGTGGDGKHTFNISTTATFI
                     IAGAGLKVAKHGNRAASSRSGSADVLLALGGNLDLDAAQVAECIEEVGIGFLYAVKHH
                     PAMRHAIGPRRELGQRTIFNLLGPLTNPAAATHQLMGVYDPTLTATLAEVLRTLGSRA
                     AYIVHGADGLDELTTTGPNRVSSLRDGQVTTFEFDPASIGLPRAQLDDLLGGTPEENA
                     AILRGILSGEDRGPRRDIVLLNAAAALSLETGDWAAGLETARHTLDSGAALATLENWV
                     AMTNRF"
     gene            4870..5181
                     /locus_tag="PLC06_10895"
     CDS             4870..5181
                     /locus_tag="PLC06_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO90994.1"
                     /translation="MTTNMLTLQDIIADIHALNEDLAMYERKYGVLSQTFYDSWQRGE
                     EPDDDAWVLDWADWAGAYKIWLRRREQYERTIEALRQQSETLVDVIERTMRRDPIPVA
                     A"
     gene            5156..5608
                     /locus_tag="PLC06_10900"
     CDS             5156..5608
                     /locus_tag="PLC06_10900"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HRO90995.1"
                     /translation="MTPFQSLRDYEEFVYTLQKQFPSILQSTLVLVPRGRRIAILQGD
                     ILFEHGFRLSIKERLSDDMGRVAIEAYGYEMWRDAEKISWYDAQPHPDVPELQATFPH
                     HKHIAPDIKHHRIPAPGMSFDHPNLPQLIMEIETILAGIVDEQVERPD"
     gene            5615..>6002
                     /locus_tag="PLC06_10905"
     CDS             5615..>6002
                     /locus_tag="PLC06_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HRO90996.1"
                     /translation="MSVTNLAKRRGEILDEIMAHHRENLPKVMADVPFADLRAMAGVA
                     PPTRDFEAAVRAPGVSLIAECKKASPSRGLLVHQYDPVRLAKLYEKAGARAISVLTDT
                     RHFQGTLAHLRDAREAVRLPVLRKDFL"
BASE COUNT         1091 a         1966 c         1901 g         1044 t
ORIGIN      
        1 aggtcgaggc cttgttgaga gagcgcacca gggcgatggc ttccggcagc aactcgtcgt
       61 ccccttcggc cacgttgccg ttgtcgtttt ccatctgctg gagcgcttcc cacagcggtt
      121 gctggagaac atcgtcgaga tgaccgccgg ctgacgcgac ccctgtcgcg cggccgacgc
      181 cggaagcgat cacctcatca gcatcttctt ccaaatccgt cacctcgtca tccataaccg
      241 gttttcggtg ggcagaagtc cgctcgtcgt tcgtacttcg tacttcgtcc ggcggtccca
      301 tcgggatctt cggcgcgcgg cgttgcgggg cgggggtggt cgtgtccgtc accggcgcgt
      361 tcaagtggga gatgggcggc ggcacgtaag cgggcggtcc cggctgggga tcgatgatca
      421 ccaccggctc cttcaccggt cgagcctgtg tgggcatcgt ctgtggaccg tggtctgtgc
      481 tcgtccgtcg tccgtcgtcc gtcgccggtc gctcgacagg agccgcaccc gctgttgctc
      541 ccggcgcggc gatcacctgc gcccggcggg ccgtctgcca atagctgatg agcctggcca
      601 gctcgccatc gctgacgaag cagccctgca ggcgcacggg tgcggccgcg tcggggcttt
      661 ggaacagcat atctccctgg cccagcagcc gctccgcgcc ggtggcgtcg aggatgaccc
      721 ggctgtcggt gctgctggcc acggcgaaag cgatacgggc cgggaagttg gccttgatca
      781 ggccggtgac cacgtccacc gacggccgct gcgtggcgat gaccatatgg atgccggtgg
      841 cgcgggccat ctgcgccagc cgagtgatgc tgcgctcggt atcttccggg gccaacatca
      901 tcaggtcggc cagctcgtcg atgacgatga cgatataagg cagcttctgg acggtggcat
      961 cgcggcgaat cttcttgttg tattccacca gattgcgcgc cccggccccg gcgaacagct
     1021 tgtagcgatt gtccatctcg cgcagcgccc attgcagcgt gccgatgaca cggtccatat
     1081 cgaccaccac cggcgcggcc agatggggaa tgccgttata accggtcagt tccacccgct
     1141 tgggatcgac catgaccagc cgcaggtcgt cgggcgtgtt ttgcagcagc aggcaggcga
     1201 tgatgccgtt gacgcacacc gacttgcccg atccggtcgt cccggcgatc agcacgtggg
     1261 gcatggcggt caggtcggcg gcgatcggtt gcccggccac attctggccc agccccacgc
     1321 gcagcggcga cttcatgcgg ccgtattcag ccgattccat cacgtcgcgc agcgagacga
     1381 gcgccttggc gctgttgggc acttcgatgc ccacgtagcc cttgccgggc acgggggcct
     1441 ggatgcgaat ggagcgggcg gccagcgcca gcgccagatc gtcggccagc ccagcgatct
     1501 tgccgacctt caccttcgtc cgcttgccgc cgcgcatgac cacatagttt ggctccacgc
     1561 cgaactgggt gatcgtcggc ccctgattga tctccactac cgtggccggc gcgccaaagc
     1621 tctccagcgt ctgctcgatc acctcgaccc gctcgcgaat cgaggcgctg ttgacgtcga
     1681 tgtcggtgcc cgattccagc acatcggaca cgcgaggcag cgtccagatg gagccgggaa
     1741 cgacggccgc cgcgggccgg tcagcctcaa taaaaacggg cggcggcgtc tctttcttgc
     1801 ctcgcttggg cttctcatga acagacacgg ccgcaccacg cgccggggct tccccttcct
     1861 ctggaataag ccgcaattga ggctccggcc ggcggtcggc ggtcggcggt cgcgcgacag
     1921 gaatcgcgtt ggcggccgcc tcccgccctg gattaatccg cacagttcgc tcggcaggct
     1981 ccgcgccagt cgacggccgc ggctcgcgct cgaaccagcc ggccacctga tcacgcgaga
     2041 ggcccgcggc caggacgacg ccggccagcc cgacgaccat caacgtggcg atggtgccca
     2101 gatcgcccag caatcgggcc agcgccgtag ccagcaacga gccgagatag ccacctccct
     2161 tgccggccgc ggccacggcg tagaggctct cataggcttg ctcgcgctga taggcgatca
     2221 tcgaggcgaa ggccaggaag acgaggaaga cgacggcggc acccagcagc cggaaccagg
     2281 gcagctccgg ctcttgttcc atgccgcgca ggacgaggaa gacgccgatg ccgccggtga
     2341 tgagcgggat gacgacgcgg ccgatgccga acgccagccc agccagcccc gacagccatt
     2401 gggtgaactg cccctggttg ggcgagagca ggctgagggt caggatggcg gtcaggaaca
     2461 ggatgacgat tccggccagc aggctgcgct ggccatcgtc gaggcgcggc ggcggagctt
     2521 ttttagcggc cgtcgatttt ttggcggcgg ggtttttggc ggcgggcttg ctgctcgaat
     2581 tgctcttcgc cttgctcgtg gcgctcgcct ggcgtttggc gcccgtctga cgagtggtgc
     2641 tcgtcttgtc gctcttgtta ctggtagcca tagctcaaat gttcctgcgg gccattatag
     2701 cacgaaatag ggcggacgac cgccgacggc tgaccgccga ccacggacga cagaccacag
     2761 caagagcgcg ctcgtcactc gtcgctcgtc actcgtccta tctcccctgc caccagtcat
     2821 tgcgcgagcg cgcgtcgagg aaaaggcccc aatcgcgcac ggccgccttc acctcattgc
     2881 ccgacgcgcg accgaacggg gcatggccgc gcagcgccgg cagcgaatgc cgtccggcgg
     2941 catgagactg gcgatagagc gccaccaggt cgggatcgac gcacacgtcc tcgatgtgga
     3001 ccacatagcc gaaaagctca tcttcgcggc cgatgtggag cgtgactcgc gtcggagccg
     3061 catagagcac cagatattca tacccgccgc cgatgtcata gccggaatcg ggcgtatgga
     3121 tcacctcgcc cggcttcgtg cccatgccca gcaacgtcac ctcccaaggg ggattggggc
     3181 cacggtcggc gttataggcg ctggtgaaaa ccggcgtccg cttgtcgcca aacatccccc
     3241 aaagctgcgg ggctttcgcg tcatgcaccg gccccagatt cttcagctgc agcgccgcat
     3301 tgattcgtat atagccgcgc acgcccaggt tcaggtcgcg gttagtctcc gggtccaccg
     3361 tcagcggcgg gccggcgatg ctgagcgtgg tgaaggacgc gccggggacg ttgcagtcca
     3421 tggccggcgg tcgctggaac atcacagcgg gcatataggc tcgcttgccc tgcgcgtcgt
     3481 cctcggccag gacaacgcct gtgccggcga cgagcatcgt ggcgatggac agcaggagcg
     3541 ccgcccatcc caatcgaaag cgcaacaagt ttctcatgtt catgcaacct ccatgagaga
     3601 cccgacgggc aaacgatctg ctatctgccc accggattcg ctaattgcaa agcaaggtga
     3661 tttgttgctt gagtcaaggc cgcgccgaag tataatctga caatcggcga gaacaacccc
     3721 cggcgggccg cgcaaaggca gggttgttag caaatgctca ccaacgacga ccgatcggcc
     3781 cgataccgac aacgaggaga acgacattgc ccctaagaac agccatcgcc aaggtcatca
     3841 atcgccagga cttgagtttc gaggaagccg ccgcagccat ggacgccatc atgggcggcg
     3901 cggccactcc agcccaaatc ggcagctatc tgaccgcgct gcggatgaag cgcgagacgg
     3961 cggatgagat cgccggcagc gccttttcca tgcggcggca cgtcattccg gtagaggtgc
     4021 cgctgagcgg cgacgagttc ctgctggaca cctgcggcac gggcggcgac ggcaagcaca
     4081 cgttcaacat ctccaccaca gcgaccttca tcatcgccgg ggcggggctg aaggtggcca
     4141 agcacggcaa ccgcgccgcc agcagccgca gcgggtcggc cgatgtattg ctggctctgg
     4201 gcggcaatct cgacctggac gcggcacagg tggccgagtg catcgaggaa gtgggcatcg
     4261 gcttcctcta tgccgtgaag caccacccgg ccatgcgcca cgccatcggc ccgcggcgag
     4321 agctgggcca gcgcaccatc ttcaacctgc tggggccgct gaccaacccg gccgcggcca
     4381 cccaccagct catgggcgtc tatgacccga cgctgaccgc cacgctggcc gaagtgctgc
     4441 gcacgttggg cagccgcgcg gcctacatcg ttcacggggc cgacgggctg gacgaactga
     4501 ccaccaccgg ccccaaccgc gtcagttccc tgcgcgatgg gcaggtgacg accttcgagt
     4561 tcgacccggc gagcatcggc ctgccgcgcg cccagctaga cgatctgctg ggcggcacgc
     4621 cggaagagaa cgccgccatc ctgcgcggca tcctgagcgg cgaagatcgc ggcccgcggc
     4681 gggatatcgt gctgctcaac gcggcggcgg cgttgagcct ggagacgggc gattgggccg
     4741 ccggcctcga aacggcccgc cacacgctcg acagtggcgc ggcgctggcc acgctggaga
     4801 attgggtggc gatgacgaac cgcttctgag aatgctcagc tatggtatac ttattgcgag
     4861 gtgaacaata tgacaaccaa tatgctcact cttcaggaca tcattgccga cattcacgct
     4921 ctgaatgaag atctcgccat gtatgaacgc aagtacggcg ttctctcgca aactttttat
     4981 gactcctggc agcgtggcga ggaacctgat gatgacgcct gggtgctgga ttgggcagat
     5041 tgggcgggcg cctacaagat ctggctccgc cgacgcgaac aatacgagcg gacgatcgag
     5101 gctttgcgtc aacaatccga aacgctggtc gacgtcatcg agaggactat gagacgtgac
     5161 cccattccag tcgctgcgtg attacgaaga gttcgtctat acgctacaaa aacagtttcc
     5221 ttccatcctg caatccactc tggttcttgt accacgtgga cggcgaattg ccatcttgca
     5281 gggagatatc ctgtttgagc atgggtttcg cctgagcatc aaggagcgct tgtctgacga
     5341 tatgggccgc gtcgccattg aggcttatgg gtatgaaatg tggcgggatg cagagaaaat
     5401 atcgtggtat gatgctcaac cgcatcccga cgttcccgaa ttgcaagcaa cattcccgca
     5461 tcataaacat attgcacctg acatcaagca tcaccgcatc ccggcccccg ggatgagttt
     5521 tgaccatcca aacctgcctc agcttatcat ggaaattgaa acaatattgg caggaatagt
     5581 tgacgaacaa gtcgaacgac ctgactgaaa acttatgagc gtcaccaacc tggccaaacg
     5641 tcgcggcgaa atcctcgacg agattatggc ccaccatcgc gagaacctgc ccaaggtcat
     5701 ggccgacgtc ccgttcgccg acctgcgggc catggccggc gtggccccgc ccacgcgcga
     5761 cttcgaggcg gccgtgcgcg cgcccggcgt ctccctcatc gccgaatgca agaaggcctc
     5821 gcccagccgc gggttgctgg tccatcagta cgaccccgtc cggctggcga agctctatga
     5881 gaaggcgggc gcgcgggcta tctccgtgct gaccgacacc cgccacttcc agggcacgct
     5941 ggcccatctg cgcgacgcgc gcgaggcggt gcgcctgccg gtgctgcgca aggatttcct
     6001 ct
//
LOCUS       DAOMQG010000198         5982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5613_length_5982_cov_11.420618, whole genome shotgun sequence.
ACCESSION   DAOMQG010000198 DAOMQG010000000
VERSION     DAOMQG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_5982_cov_11.420618"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..280
                     /gene="ssrS"
                     /locus_tag="PLC06_10910"
     ncRNA           103..280
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLC06_10910"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(408..1295)
                     /locus_tag="PLC06_10915"
     CDS             complement(408..1295)
                     /locus_tag="PLC06_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRO90997.1"
                     /translation="MRVKVVLNPYANRWGAKAQADETAEAFRAAGVECDLVVTETARQ
                     GVSIAEAAAREGYDAVVAAGGDGTINEVINGLIRAAGDGPTVPFGIIPLGTANDFNLM
                     AGLPDAVEDSVRVIAAGKTRRIDAGQVNDRFFINNSAAAMEPMVTIENIKMTRLSGEI
                     RYIVALLRALLKLKPWQMKLTWDGGGYEGPAYLLSVCNSPRTGGFMMAPGAELDDGLL
                     DMVFAPQVSKGAVISILLKLMQGKHIHHPAVTFKRITAIDLTSTPGTPLHSDGELFAE
                     SAETVSYRVLPGKVTLLTP"
     gene            complement(1324..>1860)
                     /locus_tag="PLC06_10920"
     CDS             complement(1324..>1860)
                     /locus_tag="PLC06_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRO90998.1"
                     /translation="VDRTCNGTGRVTEMTLAELRELTIDGKHTIPTLDELFETLGRRT
                     LYNVELKILKVNDGGLAAAVADRIRKHDLGDKVIVSCFSPFVVREARRRLPRAIPVAH
                     LREHRLMQLAHTFVRAEADHPDYALVDESMMAWARRRGLRVNVWTVDDPAEARRLIEL
                     GVNGIITNRPGALRATLA"
     assembly_gap    1861..1960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1961..2646)
                     /locus_tag="PLC06_10925"
     CDS             complement(<1961..2646)
                     /locus_tag="PLC06_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRO90999.1"
                     /translation="MARTKIVATIGPGSAHAETIRRMLDAGMTVARINFSHGDHAGHT
                     RTVEMLRAVAAEEGKTLAILGDLQGPKLRLGHVKAGGMMLSPGDEIVLTPHPGQPAMI
                     HLPHPDLIAAAQPGDRLVIGDGEVEFTVAEKRGDTLRVIVSVAGLLESRKGVNAPGVD
                     LPISCLSDKDHEDLDLICELDFDYVALSFVRTVYDIKELQDLMRDKPRQIPIVAKIEK
                     KEAIDNLLAIR"
     gene            complement(2673..3767)
                     /locus_tag="PLC06_10930"
     CDS             complement(2673..3767)
                     /locus_tag="PLC06_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91000.1"
                     /translation="MQPSKEFSGLTMPVFSAFGWAGEENALKFALAQLQLFIDALYLR
                     LPPDVRDDFSSRGLSPDNQNVYLATGDSFDKEAYVAFNARPMSLEVQLGIVGTNLLSK
                     GLAAVNKDLTVAHHTLTQLDPNWTLRVQQMQIDPDSQERAHHLDLFKDSVNNLSEDQA
                     REIFSRAAYLNEEDKWATPIYLSLRLPSERVAAMSTAVVDISADLVATLMPTLRLFTG
                     KKTRKARATRSTTKAPRRPAEATEETPPGEQAITGSIKAMADSFTYVAELKPLHIRRG
                     FVNLTAAHWPFFAASNRMETRDVTVVFGGRQDRHSSVWRLQPGDQARLVLGPQVHEWL
                     EENFANSENIRVVARRLDNDEIRITLDAAG"
     gene            3758..3997
                     /locus_tag="PLC06_10935"
     CDS             3758..3997
                     /locus_tag="PLC06_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91001.1"
                     /translation="MVAYDLSGGKFDCGSVQAPIRRGMIFPYRIDTSPGAPNVKLNCS
                     QLLSGFPDDEPGRSTLKGIGSTFLADRPALSDPVI"
     gene            complement(3990..5690)
                     /locus_tag="PLC06_10940"
     CDS             complement(3990..5690)
                     /locus_tag="PLC06_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91002.1"
                     /translation="MNSDRCILVLGGAGLVGSQIVREIARELEPARIVVASLYRGEVR
                     EFLHDARREFCNVEFIGAWGDVFVRDEFKEENRRRLIQTRVRRDALYQDLFGGLDEAY
                     ERSGLAQLIHQYKPNVIVDCINSATAISYQDIESLSKQTHDLIDQLNQIVDHQDLTAL
                     SNLGRDLEQNVSSLLISQSLPQLIRHVQIIHRAMSDNGTRLYLKVGTTGTGGMGLNIP
                     YTHSEDRPSFKLMSKTAVAFAHTGLMFLMARTPNGPLVKELKPGAMIGYRRVAFKTVK
                     LRGRPLFRYQSQEQPLVDRLLLRGDENAYERLDKLQMAGVDTGENGFFARGEFETITH
                     TDQMEFITPEEIAQQAVLEIKGSNTGYDVIAGIDSSILNPSYRAGVLRQTALDKMARI
                     EEETQTHSVALGQLGPPELSKLLYEAHLLKLNYGTLERVLVTPPDEISRTLYEFILRE
                     ENLRTTITSIGLPILTPAGDCLLRGPRLNIPESIYREIEIDEAHINAWATKGWVDLRP
                     VNFRAWHNRFERMLRTKHMLHTRGTSSVTMKTYLPETIEIGAVVAWVFMNEDDGYRIK
                     "
BASE COUNT         1084 a         1768 c         1824 g         1206 t
ORIGIN      
        1 acccactgac ccactgaccc actgacccac tgacccactg acccactgac ccactacgtg
       61 taaagtccta ttgccgccta tagaggactg tgctactatg tgctcaccct gctgtgtgcg
      121 agagtgcctc tagcgttgag ccactttgat ttccctggga attgggatac gtaagagggg
      181 atgtagtagc cgcaaggcaa ggcagtgtac ctcgagacaa aattcccacc tgcgagagca
      241 ggttcataca atagagcatt cacggcatgg tggggcgaac tcatcgcaac cctatattcc
      301 ccgtttattc aactcatgga ccgtttcacc gggcttcctg aagggaaagc tcgacgtgaa
      361 acgctgtcta tttgccgggc ctgccgcgcg agtgatgacg gcttgggtca gggcgtgagc
      421 agcgtcacct tgccgggcag cacgcgatag ctcaccgtct cggccgactc ggcgaaaagt
      481 tctccgtcgc tatggagtgg cgtgccggga gtgctcgtca gatcaatggc cgtaatgcgc
      541 ttgaatgtga ccgccggatg atggatatgc ttgccctgca tcagcttgag cagaatggag
      601 atgaccgcgc ctttcgacac ctggggcgca aagaccatgt ccagcaagcc gtcgtcgagt
      661 tccgcacccg gcgccatcat gaagccgccc gtgcgcgggc tgttacacac cgacaacaga
      721 taggccgggc cttcatagcc gccgccgtcc caggtcagct tcatctgcca cggcttcagc
      781 ttcagcaacg ctcgcagcag ggcgacgata tagcgtatct cgcccgaaag tcgagtcatc
      841 ttgatgttct caatcgtcac catcggctcc atcgccgcgg cgctgttgtt gatgaagaag
      901 cgatcgttaa cctggcccgc gtcgatgcgc cgcgtcttgc cggcggcgat gacgcgcacg
      961 ctgtcctcga cggcgtcggg caaaccggcc atcaggttga agtcgttggc cgtgcccagc
     1021 ggaatgatgc cgaaaggcac ggtcggcccg tcgccggcgg cgcggatgag gccgttgatg
     1081 acttcgttaa tggtgccgtc gccgcccgcg gctaccaccg cgtcgtagcc ctcgcgcgcg
     1141 gccgcctcgg cgatggatac cccctgcctc gctgtttcgg tgaccaccag atcgcattcg
     1201 acgccggccg cgcgaaacgc ctcggccgtc tcgtcggcct gggctttggc cccccagcgg
     1261 ttggcatacg gattcaggac aactttaacg cgcatgatcg tgacctcatc gcaaggataa
     1321 ttctcaagcc agggtcgctc gcagcgcgcc cggccgattg gtaataatac cattgacgcc
     1381 cagctcgatc agcctgcgag cctcggccgg atcatcaacc gtccacacgt tgactcgcag
     1441 gccacgccgc cgcgcccagg ccatcatcga ctcatcaact aatgcgtagt cgggatgatc
     1501 cgcttcggcg cgcacaaagg tgtgggccag ctgcatcaga cgatgctcgc gcagatgagc
     1561 caccgggatg gcgcgcggaa ggcggcgacg agcctcgcgc accacgaacg ggctgaagca
     1621 cgagacgatc accttgtcgc ccaggtcatg ctttctgatg cggtcggcca cggccgcggc
     1681 cagcccgccg tcattcacct tcaggatttt caactccacg ttgtagagtg tgcggcggcc
     1741 gagcgtctcg aacagttcat ccagcgtcgg gatggtgtgc ttgccgtcaa tcgtcagctc
     1801 tcgcagctcg gccagcgtca tttcggtcac gcggccggtg ccgttgcagg tgcgatcgac
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgaatggcca gcaggttgtc
     1981 gatggcctct ttcttttcaa tcttggcgac gatcgggatc tgccgcggct tgtccctcat
     2041 caggtcctgc aactctttaa tgtcgtagac ggtgcgcacg aacgacagag cgacatagtc
     2101 aaaatccagt tcgcagatca ggtcgaggtc ctcgtggtcc ttgtcggaga ggcaggagat
     2161 gggcagatcc acgcccgggg cgttcacccc tttgcgcgat tcgagcagcc cggccacgct
     2221 gacgatcacc cgcaacgtat cgccgcgctt ctcggccacg gtgaactcga cttcgccgtc
     2281 gccgatgacc agtcggtcgc ccggctgggc agcggcgatg agatcggggt gcggcagatg
     2341 gatcatcgcc ggctggccgg ggtgcggcgt cagcacgatc tcgtctcccg gagagagcat
     2401 catgccgccg gctttgacat gacccagacg cagcttgggg ccttgcaggt cgccgaggat
     2461 ggccagagtc ttgccttcct cggcggccac tgcccgcagc atctccacgg tccgggtgtg
     2521 cccggcgtga tcgccatggg agaagttaat acgggccacg gtcatgcccg cgtcgagcat
     2581 gcggcgaatc gtttcggcat gggccgaacc ggggccaatg gttgcaacta ttttggtgcg
     2641 cgccatatgt cttcaggaga gcgatctgag ccttagccgg ccgcgtcgag ggttattctg
     2701 atttcgtcgt tatcgagacg gcgcgccacc acccggatat tctcgctatt ggcgaaattc
     2761 tcttccagcc attcatgcac ctgggggcca agcaccagac gggcctgatc gccgggctgc
     2821 aagcgccata cgctgctgtg gcgatcctga cggccgccga acaccaccgt cacatcgcgc
     2881 gtttccatgc gattgctggc ggcgaagaac ggccaatggg ccgcagtcag gttgacgaat
     2941 ccgcgccgaa tgtggagcgg cttcaactca gccacatagg taaaactgtc ggccatggcc
     3001 ttgatgctgc cggtgatggc ctgctcgccg ggcggggtct cctctgtggc ctctgccggc
     3061 cggcgaggag ccttggtggt ggatctggtg gcgcgggcct tccgggtctt cttgcccgtg
     3121 aagaggcgca acgtcggcat caacgtggcc accagatcgg ccgaaatatc gaccacggcc
     3181 gtgctcattg ccgccacgcg ctccgaggga agccgcaggc tcaaatagat tggcgtcgcc
     3241 catttgtcct cttcgttgag ataggcagcg cgagagaata tttcgcgggc ctgatcttcg
     3301 ctcaggttat tgacgctgtc cttgaacaga tccagatggt gggcgcgctc ctgggaatcg
     3361 gggtcgattt gcatctgctg cacgcgcaac gtccagttgg ggtccagttg ggtcaatgta
     3421 tggtgggcga ccgtcaggtc cttgttgaca gccgccaacc ccttggacag caggtttgtg
     3481 ccgacgatgc cgagctggac ttccaggctc atggggcgag cgttgaacgc cacataggct
     3541 tccttgtcga aactgtcgcc ggtggccaaa tagacgttct ggttgtcggg acttagaccg
     3601 cgcgaggaga aatcatcgcg cacatcaggg ggcaggcgca gataaagcgc gtcgatgaac
     3661 aattgcagtt gcgccaaggc aaatttgagg gcattttctt cccccgccca gccaaatgcc
     3721 gaaaagacag gcatcgtaag gccggagaac tctttagatg gttgcatatg atctctctgg
     3781 tggtaagttc gattgcggat cggttcaagc gccgatccga agaggaatga tttttccgta
     3841 tcgaattgat actagcccag gcgcgccaaa tgtcaagctc aattgctctc aacttctatc
     3901 cggcttcccg gatgatgaac ctggccggtc gacgctgaag ggtataggca gcaccttcct
     3961 ggccgaccgg ccggctcttt ccgatcctgt tatttaatcc ggtagccgtc atcctcgttc
     4021 ataaagaccc aggccacgac ggccccgatc tcgatcgttt ccggcagata ggttttcatc
     4081 gtcaccgatg acgtgccgcg ggtatggagc atatgcttgg tacgcagcat gcgctcgaag
     4141 cgattgtgcc aggcgcggaa gttgacgggc cgcaggtcga cccagccctt ggttgcccag
     4201 gcgttgatgt gtgcctcatc gatctctatc tcgcgataga tgctctccgg gatattgaga
     4261 cgaggcccgc gcagcaggca atcgccggcg ggagtgagaa tgggcaggcc gatcgacgtg
     4321 atcgtcgtgc gcaggttctc ctcgcgcagg atgaactcat acaacgttcg ggagatctca
     4381 tccggtggcg tcaccagcac gcgctccagc gtgccgtagt tgagcttcag cagatgcgct
     4441 tcatagagca gtttggacag ttccggcggc ccaagctgcc ccagggcgac gctgtgggtt
     4501 tgcgtctctt cctcgatgcg ggccatcttg tccagcgccg tctgtcgcag tacgccggcg
     4561 cggtagctgg ggttcaggat gctgctgtcg atgccggcga tcacgtcgta accggtgttg
     4621 ctgcccttga tctccagcac cgcttgctgg gcgatctctt ccggtgtgat gaattccatc
     4681 tggtcggtat gggtgatcgt ctcaaactcg ccgcgggcga agaagccgtt ttcgccggtg
     4741 tcgacgccgg ccatctggag tttgtcgagg cgctcatagg cgttctcgtc gccgcgcagc
     4801 aatagccggt cgaccagcgg ttgctcctgg ctttgatagc gaaacagcgg ccgcccgcgc
     4861 aacttcaccg tcttgaaggc cacgcgacga tagccgatca tcgctcccgg cttcagctct
     4921 ttcactagcg gcccgttggg tgtgcgggcc atcaggaaca tcaggccggt gtgggcgaag
     4981 gcgacggccg tcttgctcat cagcttgaag ctgggccggt cttcgctgtg ggtatagggg
     5041 atattgagac ccatgccgcc cgtgccggtg gtgccgactt tcagatagag gcgcgtgccg
     5101 ttgtcggaca tggcgcgatg gatgatctgc acgtggcgga taagttgcgg caacgattgg
     5161 gagatgagca gcgagctgac gttctgctcc agatcgcggc ccaggttcga cagcgcggtc
     5221 aggtcctgat gatccacgat ctggttgagc tgatcgatca gatcatgggt ctgcttgctc
     5281 aggctctcga tgtcctgata gctgatggct gtggccgagt tgatgcaatc gacgatgacg
     5341 ttgggcttgt actgatggat gagttgggcc agaccggagc gctcgtaggc ctcgtccagc
     5401 ccgccgaaga ggtcctggta gagcgcgtcg cgccggacgc gggtttgtat cagacggcgg
     5461 cggttctctt ccttaaactc gtcgcgcacg aagacgtcgc cccacgcgcc gatgaactcg
     5521 acattgcaga actcgcgccg cgcgtcgtgg agaaattcgc gcacttcgcc gcgatagagg
     5581 ctggcgacga cgatgcgcgc cggctccagc tcgcgggcga tctcgcgcac gatctgcgag
     5641 ccgacaaggc cggcgccgcc gaggacgagg atgcagcggt cgctgttcat cacgcccccc
     5701 tcatggatat gaattccaca ggttgcatcg aatggcaaaa agcaggggca gacacctgcc
     5761 tcggtcgccg ctcccataag aaattgacgc ttgcgctcat gacttgctcc gggtcgatct
     5821 ggacatggtg aagtgggtag agtttgattg tccctcggat caggggcagg ggcaaccggc
     5881 caaaacaaaa acagccgcgc gtggcggctg cttgtgggcg atacaagact cgaacttgtg
     5941 acctctacga tgtcaacgta gcgctctaac caactgagct aa
//
LOCUS       DAOMQG010000199         5953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5647_length_5953_cov_9.589861, whole genome shotgun sequence.
ACCESSION   DAOMQG010000199 DAOMQG010000000
VERSION     DAOMQG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5647_length_5953_cov_9.589861"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLC06_10945"
     CDS             <1..336
                     /locus_tag="PLC06_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91003.1"
                     /translation="VTFVHTTWDRFKDATEINDLFIEGGPYVDRLYATLKERGIHPER
                     NYHVDEGTDEHVVALSVPTRDGRVNVSYGDLPASDRALMALAEALVQEIARQGGLAEW
                     RETPPLSTK"
     gene            404..1504
                     /locus_tag="PLC06_10950"
     CDS             404..1504
                     /locus_tag="PLC06_10950"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HRO91004.1"
                     /translation="MTPDTPPIPDDDSQSAPGASYEPVWTFRGYKMRPSEFNTAMTHY
                     YRAEIQRSNVWRSRLDTTTNWAVVTVAAAVTFVFAEPGNHWGVFLLVILLTLLFLSIE
                     ARRYRYYELWSLRARLMETDFFAAMLVPPFAPHAEWNETLAESLLQPEFPISRLEAIG
                     RRLRNNYLAIFSILQAAVLMKLYLHPTEAQSLGELVSRAGIGPLYGGVVLSIGLGFLA
                     VLFLIAYITRDLHEASGEVVPKYDMEDLLGNLWPANGQEKERSTSRAMQSRLSVRKRR
                     RQQVMALVVAADPDKVADRVIREMKRGVTSLHGRGMFLKQDREVLLVALTVTEIEQLK
                     VIVREEDTHAFITVIPANEIVGQGFMPLEEEN"
     gene            complement(1579..2994)
                     /locus_tag="PLC06_10955"
     CDS             complement(1579..2994)
                     /locus_tag="PLC06_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91005.1"
                     /translation="MKDVFMKRLVHLLWLLPLMLAACAKPGTNQVVPAITPVVMATAE
                     TTGMLVVETAVPTLAPAATEAPVLMCTPPACAAGEGYACPSGDCPGGCGTVCVAPTPV
                     TGPLAPAPTDWESLEGWLTTLWQNNVNPAAVRAALQASGMQKSLSDWAAADFDGDLMD
                     EWVLVLYDQSLPGLPFGVAGDLWIVNGDGVVFRYHDAPSNDIFQFLAPSIIAMTDMTG
                     DGLPEIITDAPVCGASTCTNNYRIIGPQDGRIADLVVAPPVAEVEPSGQVISVPYADT
                     RIEDVDADGLLELLVHGGTLGSAGAGIVRPRTEVWGWDGAAVTRTDIILDPTAYRHHI
                     LYEANDLMASGDLARAMPLYEAVINDNALRDDGYSYPPEQVRADISAFAAFRLILIDR
                     LIGDSERAASRLAWLLATYPDSPVAGAADRLIREWGTYGNSTELCAQIEGGLATLENP
                     TGSLADMGYGNPGLGAADLCP"
     gene            complement(3009..4904)
                     /locus_tag="PLC06_10960"
     CDS             complement(3009..4904)
                     /locus_tag="PLC06_10960"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO91006.1"
                     /translation="MEKKQMINRPTVKGLLFYIAALGVLLLARPVGARQVQGERTHVR
                     ITAVDSAAFPTVKVRVLTTTAGSGPVEDLSRLVLRENGVPIPEATAAQTSVGVDVVLV
                     IDANADFLLFDDNSGLSRRDQLAAGIARFAEQFMNPDGLDRVSIIVPDETGAGAAFLL
                     TDAARPGDVAAAINTYNPVAPGATPLQEMMTAAIEHLAGSEDGRFQAVLLYTDGARLP
                     RQLDYPTLVEATQAARIPIYVAILGADASAEEIANATGLFDPTNGAYVHMPNPEAGDP
                     IYAIFEAQGQQTELSYQSALRQNGTHQVSVSLGNVRDAAEFELLLAAPEITVEPPPSS
                     VRRAGSAIDTPLALLQPAVLPLTARINWPDGQPRQIVEVLFRVNDVAQPLATLPVVDA
                     GGSLPLAWDISQSDAGVYRLEIEIVDELGFRVAAEPLEIAIEVARPSPPTPTPAPTRV
                     PAPPLSKRLDGLLLPVLLILALAVVAGGAIVGLRRRAAKSPSAAEVAVPRIVPAAESR
                     PADGHVAVLAWQAKDGSLGDQIELTAADVTLGCDPEAVDIIIEAPGISRLHARIRRTA
                     TEEYWLYDEGSAAGTFLNYERLALAPKMLQHNDLVQLGQATLRFRLELPAQGEGEQVA
                     QATSNDR"
     gene            4956..>5953
                     /locus_tag="PLC06_10965"
     CDS             4956..>5953
                     /locus_tag="PLC06_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91007.1"
                     /translation="MRHSVGRLLRSIPALLALVLGVVTIALAQGTNQIGELFITDSNT
                     EGFPSIRLQLFGIDGQGLPIDFATEPLFITHGGFPVEEVVFDGKTPVGTLTVFLIDAA
                     GGATDQIPAIKAAIQQYASPGNMQEQVDYVAVYQIRSGGPQQLLAPTQFHNGVANLFN
                     TTELVPEEGATSLYDSVITMVGEIDGLKPRPDIAASIVLISDGTDPGTSQAQPGDVSA
                     RAVAAGVPIHTIHLEDPGLGAGVELGRQYMRDVAGSSRGVAAELADPAGLASIWARIA
                     GLRDHSWVRYNVPEPAGGTIPVEVSLLNNRDTKATTEVIISTAAPNVVLDVPRESRT"
BASE COUNT         1079 a         1939 c         1860 g         1075 t
ORIGIN      
        1 gtcacgtttg ttcacaccac ctgggatcgc ttcaaggatg cgacggagat caacgacctg
       61 ttcatcgaag gcggtcccta cgtcgaccgg ctatacgcga ccctgaagga gcgcggcatc
      121 caccccgaac gcaactacca tgtcgatgag gggacggatg agcatgtcgt ggccctgtcc
      181 gtgcccacgc gcgacggccg cgtcaacgtg tcctacggcg atttgcccgc gagcgacagg
      241 gcgctgatgg ccctggccga agctctggtg caggagatcg cccggcaggg cggattggcc
      301 gaatggcggg agacgccgcc gctttccacc aaatgagatc gaccggcggc acatagcccc
      361 ggcgcttgac tttctgtaat caaatcatgg catggatgat ccgatgaccc ccgacacccc
      421 acctatcccc gatgacgaca gccaatccgc gcccggtgcg tcctacgagc cggtctggac
      481 attccgcggc tacaagatgc ggccgtcgga gttcaacacg gccatgaccc actattaccg
      541 ggccgagatc cagcgctcca acgtctggcg ctcccggctg gacacgacga ccaattgggc
      601 cgtcgtcact gtggccgcgg ccgtcacctt cgtttttgcc gagccgggca atcattgggg
      661 cgtattcctg ctggtcatcc tgttgacctt gctctttctg tccatcgaag cgcggcgcta
      721 ccgttattac gagttgtgga gcctgcgcgc acgcctgatg gagaccgact tcttcgccgc
      781 catgctcgtg ccgccgttcg ccccccatgc ggaatggaac gagacgttgg cggaatccct
      841 cttgcagccg gagtttccca tctcgcggct ggaagcgatc ggccgtcgcc tgcgcaacaa
      901 ctacctggca atattttcca ttctgcaagc ggccgtcctg atgaagctct atctccaccc
      961 gactgaggcc caatcattgg gggagctggt ctctcgcgcc gggattggcc ccctgtatgg
     1021 cggcgtggta ttgtctatcg ggcttggttt tctggcggtg ttgtttctga tcgcctatat
     1081 cacccgcgac ctgcacgaag ccagcggcga agtcgtgccc aaatatgaca tggaagatct
     1141 gttgggcaat ctgtggccgg ccaatggcca ggaaaaggag aggtcgacat ccagggcgat
     1201 gcagtcacgc ctgtccgtgc gcaagcgccg ccggcaacag gtgatggcgc tggtggtggc
     1261 ggccgacccc gacaaggtgg ccgaccgggt gatccgcgag atgaagcgcg gcgtgacctc
     1321 tctccacggc cggggcatgt tcctcaagca ggatcgcgag gtgctgctcg tcgctctgac
     1381 tgtgaccgag atcgaacagc tcaaagtgat cgttcgcgaa gaagacaccc acgccttcat
     1441 caccgtcatc cccgccaacg agatcgtcgg gcagggattc atgccgctgg aagaggagaa
     1501 ttaggaatta aagagggtgg ggctgctaca ccactcgtcg gctgttcccc cctcttcagc
     1561 tttcaaccag atcgaacgtt agggacataa gtcggccgcg cccaggccgg gattgccgta
     1621 ccccatatcc gccagcgatc cggtcgggtt ctccagcgta gccagacccc cctcgatctg
     1681 cgcgcacagc tcagtcgaat ttccgtaggt gccccactcc ctgatcagac gatccgccgc
     1741 gccggccacg ggtgaatcgg gataggtcgc cagtagccac gccaaccggc tggcggcccg
     1801 ttccgaatcg ccaatcagcc ggtcgatgag gatcagccgg aaggcggcga aggcgctgat
     1861 gtcggcccga acttgctccg gcggatagct atagccgtca tcgcgaagcg cattgtcgtt
     1921 gatcaccgcc tcatagagcg gcatcgcgcg ggccagatcg ccggaagcca tcagatcgtt
     1981 ggcctcgtag aggatgtgat gccggtacgc agtcgggtcc aggatgatat ccgttcgggt
     2041 gacggccgcg ccgtcccaac cccacacctc agtgcgcggc cgcacgatcc ccgccccggc
     2101 cgagccgagc gtgccgccgt ggaccaatag ctccagcagg ccgtcggcgt cgacgtcctc
     2161 gatgcgcgtg tcggcatagg gcaccgagat gacctgcccc gacggctcga cctcggccac
     2221 gggcggagcg acgaccagat cggcaatccg gccgtcttgc gggccgatga tgcggtaatt
     2281 attggtgcag gtcgacgccc cgcaaaccgg cgcgtcggtg atgatttcgg gcaagccgtc
     2341 gccggtcatg tcggtcatcg cgatgatcga gggggccaga aactgaaaga tatcgttgct
     2401 gggcgcatca tgataacgga agacgacgcc gtcgccattg acgatccaca agtcgccggc
     2461 cacgccgaag ggcaggccgg gcagcgactg gtcatagagc accagcaccc actcgtccat
     2521 taagtcgccg tcgaagtcgg ccgcggccca atcgctgagc gatttctgca tgcccgacgc
     2581 ctggagcgcg gcgcgaacgg cggccggatt gacgttgttc tgccagagcg tggtcagcca
     2641 accttccagg ctttcccaat ccgttggcgc gggagccagg gggccagtca caggcgtggg
     2701 agcgacgcag acggtgccgc agccgccggg gcaatcgccg ctggggcagg cgtagccctc
     2761 gccggcggcg caggcgggcg gcgtgcacat caggactggc gcctcggtag ccgccggcgc
     2821 gagtgtgggg acagccgtct cgacgacgag catccccgtg gtttcggccg tcgccatgac
     2881 caccggggtt atggccggga ccacttgatt cgtgcccggc ttcgcgcaag ccgcgagcat
     2941 tagcggcagc aaccagagca gatgcaccaa tctcttcatg aaaacgtcct tcatagcttc
     3001 atccttctct agcggtcatt cgacgttgcc tgagcgacct gttcgccttc accttgcgcg
     3061 ggcagctcca gccgaaaccg caacgtcgcc tgacccaatt gcaccaggtc attatgttgc
     3121 agcattttcg gagcaagggc cagtcgttcg taattcaaaa acgtgccggc agcgctgccc
     3181 tcgtcataga gccaatattc ctccgtcgcc gtgcgccgga tgcgcgcatg caggcgggag
     3241 atgccggggg cttcgataat aatatcgact gcttcaggat cacagccgag cgtcacatcg
     3301 gcggcagtca gttcgatttg atcgcccaga ctcccgtctt ttgcctgcca ggccaggaca
     3361 gccacatgac cgtccgccgg gcgcgattcg gccgcaggca cgatgcgcgg caccgcgacc
     3421 tccgcggccg agggcgattt ggccgctcgc cgccgcaggc cgacgattgc accgccggcg
     3481 acaacggcca gcgcgagaat caacagaaca ggcagcagca gcccgtcgag acgcttgctc
     3541 aatggcggcg cgggaacgcg agtaggcgcg ggggttggcg tcggcgggct ggggcgcgcc
     3601 acctcaatag cgatttccag cggttcggcc gccacgcgaa agcccagttc gtcgacgatc
     3661 tcgatctcca gtcgatagac gcccgcgtcg ctttggctga tatcccatgc cagcggcaaa
     3721 ctcccgccgg cgtcgactac gggcagcgtg gccaatggct gggcgacgtc gttgacccgg
     3781 aaaagcacct caacgatttg gcgcggctgg ccgtcgggcc aattgatgcg cgccgtcagc
     3841 ggcagcaccg ccggttgcag cagcgccagc ggtgtgtcga tggcgctgcc cgctcgccgc
     3901 accgatgacg gcggcggctc gacggttatt tccggcgcag ccagcaatag ttcgaattcg
     3961 gccgcatcgc gcacgttgcc cagactaacc gaaacctgat gcgtgccgtt ctggcgcagc
     4021 gccgattgat aggatagctc cgtctgttgc ccctgtgctt cgaagatcgc gtagatcgga
     4081 tcgcccgctt ccgggttcgg catatggacg taggcgccgt tggttggatc aaacaggccg
     4141 gtagcgttgg cgatctcctc ggccgacgca tccgcaccca ggatggcgac gtagatcggg
     4201 atgcgcgccg cctgcgtcgc ctcgaccagt gtcgggtagt cgagctgtcg ggggagccgt
     4261 gccccgtcgg tatagagcaa cacggcctgg aagcggccgt cttcgctgcc ggccagatgc
     4321 tcaatggccg ctgtcatcat ctcttgcaag ggcgttgcgc ccggcgcgac gggattatag
     4381 gtgttgatcg ccgccgccac atcgccgggt cgggcggcat ctgtcagcag aaatgccgcg
     4441 cctgcgcccg tctcgtccgg gacaatgatc gagacgcgat ccagcccatc ggggttcatg
     4501 aattgttccg cgaaacgcgc gatgccggcc gccaattgat cccgccggct caggccgctg
     4561 ttgtcgtcga acaaaagaaa atcggcgttg gcatcaatca ccaggaccac atcgacgccg
     4621 accgaggttt gcgcggccgt ggcttcgggg atgggcacgc cattctcgcg cagcacgagg
     4681 cgcgacagat cctcaaccgg cccgctgccc gcggtcgtgg tcaatacgcg cacttttacc
     4741 gtgggaaagg cggcgctgtc gacggcggtg atgcgcacat gggtgcgctc gccctgcact
     4801 tgccgagcgc cgacaggccg cgccaggagc aacaccccca gcgcggcaat gtaaaaaaga
     4861 aggcccttga ccgtcggccg gttgatcatc tgtttctttt ccacctttcg tctggtatta
     4921 tatcaacata acttcgtatg aaagggagaa gtctcatgcg acactccgtc ggtcgtctac
     4981 tccgtagcat cccggccctg cttgccctgg ttcttggcgt ggtcacgatt gccctcgcgc
     5041 agggaacgaa ccaaatcggc gagttgttca tcaccgattc caacaccgag ggtttcccga
     5101 gtattcggct gcaactcttc gggattgatg gccaaggatt gccgatcgat tttgctacgg
     5161 agccgttgtt tatcacccat ggcggctttc cggtggaaga ggtggtcttt gacggcaaaa
     5221 cgcccgtcgg cacgctgact gtgttcctca tcgacgccgc cggtggcgcg accgaccaga
     5281 ttccggccat caaggcagcc attcagcaat acgcttcgcc gggcaacatg caggagcagg
     5341 tcgattatgt ggccgtttat cagattcgtt ccggcggccc gcaacaactt ctcgcgccga
     5401 ctcagttcca taacggcgtc gccaatttgt tcaacacgac cgagctggtg cccgaggaag
     5461 gggccacctc gctctacgat agcgtcatca ccatggtggg cgagatcgac gggctgaaac
     5521 ctcgccccga tatagcggcg tccatcgttc tcattagcga cgggaccgac cccggcacct
     5581 cgcaggccca gccgggcgac gtgagcgcgc gggccgtcgc cgccggcgtc cccattcaca
     5641 ccattcatct ggaagatccg ggattgggcg cgggggtgga actcggccgc cagtacatgc
     5701 gcgatgtggc cggcagctcg cgcggcgtgg ccgccgagct ggccgatcct gccggtctgg
     5761 catccatctg ggcgcgcatt gccggactgc gcgatcactc ctgggttcgc tacaacgtgc
     5821 ccgaaccggc cggcggcaca atcccggtag aagtcagcct gctcaacaac cgcgacacca
     5881 aggccaccac cgaggtgatc atctccaccg ccgcgcccaa tgtcgtcctc gacgtgccgc
     5941 gcgagagtcg cac
//
LOCUS       DAOMQG010000200         5950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5654_length_5950_cov_5.739440, whole genome shotgun sequence.
ACCESSION   DAOMQG010000200 DAOMQG010000000
VERSION     DAOMQG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5654_length_5950_cov_5.739440"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLC06_10970"
     CDS             <1..590
                     /locus_tag="PLC06_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRO91008.1"
                     /translation="MARSKAFDEDRALDGAMALFWEQGYEATSVRDLTARAGISSSSL
                     YATFGDKRELYGAALERYRALEQEQFAADLAVERPIRALLARMQADLIDTLLLDDGTR
                     GSFTLNAAIELGGRDPAITHRLRDHFEAISGLLAERLAEAQRTGEIAATAPPGELAAY
                     LLLGFYSLAVMVKILPDRAQLESAAAITLSVLDHT"
     gene            754..2145
                     /locus_tag="PLC06_10975"
     CDS             754..2145
                     /locus_tag="PLC06_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRO91009.1"
                     /translation="MENNQLPTPPVLSGAIPGLGHLMEFRKDRAGMALRGLDEVGRIF
                     TVKLGPQNVAVLIGPEYHRSFFMETDKKLNIGTPYKFLRAAFGEVFFIAGHEEYLRQR
                     PFVTQAFRREKMAYYITIMQREVQKWLDSLGEEGQFDIVAAMGLLTQNVAGSALMGDR
                     FMAEAGREFWDLYDDVGKSLDPVLPPNLPLPKFIRRDKARARMGEILRPIIDERRANP
                     DAYNDFLQDFVNSRYEDSGELIEDEVLLNLMLGLMFAGHETTAGQAAWNVILLLQHPD
                     YLKLVQDEVDRHTPYGQPIDGRVLHTLTHLDYATTETQRLRPSADMLLRDVDEEIEIG
                     GYRIPAGWKVQVTSEIAHRLPELFAEPDYYDPLRYAPGREEDKSDRFALIGFGGGAHK
                     CTGMNFANTEIMVITILLLQQFDLELVTRKPAVARGMGANRPSSTIIRYRRKPPAQAA
                     GVPVAAGVAVVEN"
     gene            2305..2883
                     /locus_tag="PLC06_10980"
     CDS             2305..2883
                     /locus_tag="PLC06_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO91010.1"
                     /translation="MIDFTPHRKREKTLGELVADYTVDDLRDLTNAMIDRQLALIADC
                     TDEDVVFVPEDPAAEDKYAASEADANIAWTLGHVIVHVTASSEEKAFLAAEQARGVDR
                     DGRSRYETPWETVTTIAQVRARLEESRRMRLASLDIWPDEPNLDLTITYRYLRGPMNA
                     IARYCMGLLHDDDHLEQIAEIVRQAHAVRLAM"
     gene            complement(3034..4422)
                     /locus_tag="PLC06_10985"
     CDS             complement(3034..4422)
                     /locus_tag="PLC06_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91011.1"
                     /translation="MRSLRDQFILSHILPFMIILPLAGLVILYLVEAQFMLGQLSRDL
                     EVRAALIAEAVAHQPELLADQDAARRFIDKVAPQADGEVYLLDASGRIVAADPDLEGP
                     PDAALLNAAAQSPRTTQVSMTYGIAGQTGEAVAPVIDINEQLIGIVGVRETIGDLAAT
                     FDPLRRLIAFTILGGMALGALAGYWLAGRLARPIHETTAAVGRIAGGAPAAPLPETGP
                     REMRQLAESVNTLAARLRSLEEMRRRSFANIVHELGRPLGAVLAAIEVLRGSAGADPA
                     IREELLAGVQKELTSLEPLLDDMSQLHAEATGNIRLDRQPVALSDWLHEVVPPWREAV
                     LARELTWVVEIPPDLPEANIDPRRMSQVIGNLLSNAVKYTESGGIAVYATSTAGEVAI
                     SVADTGPGIAAGDRLRVFDPFFRGETPRRTTEGLGVGLAIARSLAEAHGGRLSLESAP
                     GRGSVFTLHLPV"
     gene            complement(4431..5132)
                     /locus_tag="PLC06_10990"
     CDS             complement(4431..5132)
                     /locus_tag="PLC06_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91012.1"
                     /translation="MSRHILIVDDDALMRRSVSLHLEQQSFRTSTAASAEDALALARR
                     DPPDLILLDIGLPGMDGLTAIRHFQRDMQDVPVIFVSARRRELDTILGLELGADDYIT
                     KPFNLDILTAHVRAILRRAERSGAGPTEPEPLTVGDITIDPGAHVVTIGGRPINLTGK
                     EFAVLHALALEAGKVVSTDDLLSRVWGAEFEGEPQVVYVNIRWLREKIEADPNAPRRI
                     VNVRGVGYKLMAQES"
     gene            complement(5282..5797)
                     /locus_tag="PLC06_10995"
     CDS             complement(5282..5797)
                     /locus_tag="PLC06_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91013.1"
                     /translation="MNRIRINIFFFLSLIMIALMTFVVTSAQAASARPTTPSRVATSG
                     GLFSGNFEGDLLGDDGSNATAELEITQTGRDVISKLTIDQGLIIDGGNCGLVEVPSTT
                     QTLKGKTPTGSPRRLEAASAFKVNGIAVTIDLDADLARDGKSMTAEAKIDLPWLCGRD
                     PIITGEFTRKS"
BASE COUNT         1090 a         1864 c         1933 g         1063 t
ORIGIN      
        1 caatggcgag atcgaaggca tttgacgaag accgcgcgct cgacggggcg atggctctgt
       61 tctgggagca gggctacgag gccacgtcgg tgcgcgatct gaccgcccgc gcgggcatca
      121 gcagcagcag cctctacgcc actttcggcg acaagcgcga gctgtacggc gcggcgctgg
      181 aacgctatcg ggcgctggag caggagcaat ttgcggccga tctggctgtc gagcgcccca
      241 tccgggcgct gctggcccgc atgcaagccg atctgatcga cacgctgctc cttgatgacg
      301 gcacgcgcgg ctcgttcacg ctcaacgccg ccatcgagct gggcggccgt gatccggcca
      361 tcacccatcg gctgcgcgac catttcgagg cgattagcgg tctgctggcg gagcggctgg
      421 ccgaggccca acgaacggga gaaatcgctg cgacggcccc gcccggcgag ctggccgcgt
      481 atctgttgct ggggttctat agcctggcgg tcatggtgaa aatcctcccc gaccgggcgc
      541 aactggagtc ggcggcggcg atcacgctgt cggtactgga tcatacgtaa cgtcgctatt
      601 gtgatgcaag gacggtcgtc tgcccctcgg cgacggtcgt gtgccgggcg agccaacccc
      661 ccgacacatc ctgaacaacc tttttgataa tcaggcggga ttgccgttgg gcgcgttttg
      721 cgttcggccg cgccccgccg ggagaaattc ggcatggaaa ataatcaatt acccacacct
      781 ccggtcctgt ccggcgcaat acccggattg ggccatctga tggagtttcg caaggatcgc
      841 gccggcatgg cgttgcgcgg gctggatgaa gtcggccgca tcttcaccgt caagctcggc
      901 ccgcaaaacg tggccgtcct gattggcccc gaatatcacc ggtcgttttt catggagacg
      961 gacaagaagc tcaacatagg cacgccctac aagttcctcc gcgccgcctt tggcgaggtg
     1021 tttttcatcg cggggcatga ggaatatctg cgccagcggc cgttcgtcac ccaggccttt
     1081 cggcgcgaga aaatggccta ctacatcacc atcatgcagc gcgaagtgca gaaatggctc
     1141 gactcgctgg gcgaggaagg ccagtttgat atcgtcgccg cgatgggcct gctcacccaa
     1201 aacgtcgccg gcagcgcgct gatgggcgac cggttcatgg ccgaagccgg ccgcgagttc
     1261 tgggacctct acgatgacgt gggcaagtcg ctcgaccccg tcctaccgcc caatttgccg
     1321 ctgcccaagt tcattcggcg cgacaaggcg cgggcgcgca tgggcgagat tttgcggccg
     1381 atcatcgatg agcgccgcgc caatcccgat gcctataacg acttcctgca ggacttcgtc
     1441 aactcacgct atgaggattc gggcgagctt atcgaggacg aggtattgct caatctgatg
     1501 ctgggcctga tgtttgccgg gcacgagacg acggccgggc aggcggcctg gaacgtcatc
     1561 ctgctgctgc agcatcccga ctatctcaag ctggtgcaag acgaggtcga tcgacacacc
     1621 ccctatggcc aacccatcga cggccgcgtg ctccacaccc tgacccatct cgattatgcc
     1681 accaccgaga cgcagcgcct gcggccgtcg gccgatatgc tgctgcgcga tgtggacgag
     1741 gagatcgaga tcggcggcta tcgcatcccc gccggctgga aggtgcaggt gaccagcgag
     1801 atcgcccatc gcctgccgga gctgttcgcg gaacccgatt actatgaccc gctgcgctat
     1861 gcgcccggcc gcgaggaaga caagtccgac cgctttgcgc tgatcggctt cggcggcggc
     1921 gcgcacaaat gcaccggcat gaacttcgcc aacaccgaga tcatggtcat caccatcctg
     1981 ttgttgcagc agttcgatct ggaactggtg acgcgcaagc cggctgtggc gcgcggaatg
     2041 ggggccaatc ggccgtcgtc gaccatcatc cgctaccgtc gcaagccgcc ggcacaggcc
     2101 gccggcgtac cggtcgcggc cggcgtcgcg gtagttgaaa attaacgcgg atattgggcc
     2161 tcttcactgc ggcttgcctc tttggcctga ttgaccaaaa ccggcgtttc cgcccttgga
     2221 gacacggcaa taacttgata aaacctgtca tgacggcagc gcagccgatg tgctgccgtt
     2281 tattacatag agaggagctg ttcgatgatt gattttacac cccatcgcaa gcgcgagaag
     2341 acgctgggcg aactggtcgc cgattatacc gtggacgatt tgcgcgacct gaccaatgcc
     2401 atgatcgacc ggcagttggc gcttatcgcc gattgcaccg acgaagatgt cgtctttgtg
     2461 cccgaagacc cggcggccga agacaagtac gccgcgtccg aggcggacgc caatatcgcc
     2521 tggacactgg gccatgtcat cgtccacgtg acggcatcgt cggaagagaa ggcgttcctg
     2581 gccgccgagc aagcccgcgg ggtcgaccgc gacggccgct cgcgctacga gaccccgtgg
     2641 gagacggtga ccaccatcgc ccaggtgcgc gcccggctcg aggagagccg ccgcatgcgc
     2701 ctggcctcgc tggacatctg gcccgacgaa cccaacctcg atctgaccat cacctaccgc
     2761 tatctccgtg ggccgatgaa cgccatcgcc cgctactgca tggggttgct ccacgacgac
     2821 gaccacctgg agcagatcgc cgagatcgtc cgccaggcgc acgccgtccg gcttgcaatg
     2881 tagggcaaac tgtagagcaa tccgccggat tgccgggccg tacaccagac ctgacaggtc
     2941 ggcgatcaag ttctcgatca gacaaatagc aggtcgccga cctgtcaggt cttgatgtgc
     3001 ggggtgcgca actaaagctg cgaactacaa gccctaaacc ggcagatgca gggtgaaaac
     3061 gctgccgcgg ccgggggcgc tctccagaga caaccggccg ccgtgggctt cggccaagct
     3121 gcgagcaata gccagcccca cgcccaaccc ttccgtcgtc cggcgcggcg tctcgccccg
     3181 gaaaaacggg tcgaacaccc gcagccggtc gccggcggcg atgccggggc cggtgtcggc
     3241 caccgagatg gccacttcgc ccgcggtact cgtcgcatag acggcgatgc cgccgctctc
     3301 ggtatacttc acggcgttgc tcagcagatt gccgatgacc tgactcatgc gtcggggatc
     3361 gatgttggct tccggcaaat cgggggggat ttcgacgacc caggtgagtt cgcgcgcgag
     3421 gacggcctcg cgccatgggg ggacaacttc gtgtaaccaa tctgaaagag ctaccggctg
     3481 ccgatcgagg cggatgttgc ccgtcgcctc ggcgtggagt tgcgacatat catccagcag
     3541 cggctccagc gaggtgagtt ccttctgcac cccggccagc agctcctcgc gaatcgccgg
     3601 gtcggccccc gcgctgcccc gcagcacctc gatggccgcc agcaccgcgc ccaacggccg
     3661 ccccagctcg tggacgatat tggcgaatga gcggcggcgc atctcttcca atgagcgcag
     3721 gcgcgcggcc aacgtgttga ctgattctgc caactgtcgc atttcgcgcg ggccggtttc
     3781 gggcaatggg gcggccggcg cgccgccggc gatgcggccc acggcggcgg tcgtctcatg
     3841 gatcggccgc gccagacgac cggccagcca atagccggcc aatgcgccga gggccatgcc
     3901 gcccagaatc gtgaaggcga tcaaccgccg caaggggtcg aaggtcgccg ccagatcgcc
     3961 gattgtctcg cgcacgccga cgatgccgat gagctgctcg ttgatgtcga tcaccggcgc
     4021 gactgcctcg cccgtttgcc cggcgatgcc gtaggtcatc gagacctggg tcgtgcgggg
     4081 tgattgggcg gccgcgttca gcagcgctgc gtcgggcggg ccttccagat cgggatcggc
     4141 ggcgacgatt cggccgctcg cgtcaagcaa atagacctcg ccgtcggcct gcggggcgac
     4201 cttgtcgatg aaccggcgtg cggcgtcctg atcggccagc aattcgggct ggtgggccac
     4261 ggcctcggcg atgagcgccg cgcgcacctc aaggtcgcgc gacagttggc ccagcatgaa
     4321 ttgcgcctcg accagataga ggatgaccag ccccgccagt ggcaggatga tcatgaaggg
     4381 caggatgtga ctgaggatga actggtcgcg caggctccgc atacttcctc tcagctttcc
     4441 tgggccatca gcttgtagcc gacgccgcgc acgttgacga tgcggcgcgg ggcgttgggg
     4501 tcggcctcga tcttttcgcg tagccaacga atgttcacat agaccacttg cggctcaccc
     4561 tcgaactccg cgccccacac ccggctcaac aggtcgtcgg tgctgacgac cttgcccgct
     4621 tccagggcca gggcgtggag aacggcgaac tccttgccgg tcaggttgat cggccgcccg
     4681 ccgatggtga cgacgtgggc gcccgggtcg atggtgatgt cgccgacggt caacggctcc
     4741 ggctcagtgg ggccggcgcc gctgcgctcc gcccggcgca ggatggcgcg gacgtgggcg
     4801 gtcaggatgt cgagattgaa cggcttggtg atgtaatcgt cggcccccag ttcgaggccg
     4861 aggatggtgt ccagctcgcg gcggcgggcg gagacgaaga tgaccggcac gtcctgcatg
     4921 tcgcgctgga agtggcggat agccgtcagg ccgtccatgc cgggcaggcc gatgtcgagg
     4981 aggatcaggt cgggcgggtc gcggcgggcc agcgccagcg cgtcttcggc cgaggcggcg
     5041 gtggaggtgc ggaagctctg ctgctccaga tggaggctaa cgctgcggcg catgagggcg
     5101 tcgtcgtcga cgatgaggat atggcgactc atggccatga ttctagctca atctatggat
     5161 ggcggtaatt gcaaactcac cctaaggaaa catcgtttgt agtagcggct ttagccgcgc
     5221 tcttcgtttg aagccaaccg cagacagcgc taaagcgcct gactacgaac caaataaatt
     5281 ttcaactctt gcgggtgaat tcgccggtga tgattgggtc gcggccgcac agccacggca
     5341 ggtcgatctt cgcctcggcg gtcatcgact tgccgtcgcg cgcgaggtcg gcgtcgaggt
     5401 cgatggtcac ggcgatgccg ttgaccttga aggccgacgc cgcttcgagg cggcggggcg
     5461 accccgtggg ggtcttgccc ttcagcgttt gagtggtgga tggcacttcg accaggccgc
     5521 aattcccgcc atcgatgatt aagccttggt caatggtcag cttgctgatg acatcgcggc
     5581 cggtctgggt tatctccagt tcggcagtcg cgttgctccc gtcatctccc agcaagtctc
     5641 cttcaaagtt ccccgaaaac agtcccccag aggtagccac ccgcgagggg gtggtgggac
     5701 gggccgatgc ggcctgggcc gaagtgacaa caaacgtcat caacgcgatc atgataagtg
     5761 acaggaagaa aaagatgttg atacggatac ggttcatggt gcgataatgc tcctgatcac
     5821 ctgattgcgt catgcgctaa tgatgacaac aggatatcgc acgatcggcg gtcgcgactt
     5881 taagggtgaa tggggttggc ttaaggggag cttaaatgga attacgaatt acgaattacg
     5941 aattacgaat
//
LOCUS       DAOMQG010000201         5925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5677_length_5925_cov_10.348041, whole genome shotgun sequence.
ACCESSION   DAOMQG010000201 DAOMQG010000000
VERSION     DAOMQG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_5925_cov_10.348041"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLC06_11000"
     CDS             <1..546
                     /locus_tag="PLC06_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91014.1"
                     /translation="PPDGDRSDEILVFNRGSLSTPFEVTKAMSGFIEDKPPTGPANAN
                     WVTGQYAGFADGTLYYRARVVGIPVDQPGMKFGFCFWENGYVNEECRGQEFDAIPGTE
                     MTWSHALSDMWVKSPINWANPRTKHGIVVRNQNNKPVSAKKGWNWYGENPDHWYPMYI
                     HYTVVLVKDGGTFSGWDYYGW"
     regulatory      675..793
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            845..4576
                     /gene="metH"
                     /locus_tag="PLC06_11005"
     CDS             845..4576
                     /gene="metH"
                     /locus_tag="PLC06_11005"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRO91015.1"
                     /translation="MNRTEQLHQQLAQRILVLDGAMGTMIQQYHLSEADYRGARFADW
                     PRDLQGNNDLLVLTQPDIIRAIHLAYLEAGADLIETDTFNANGISQGDYGMEELAYEM
                     NVAAARLACEARDEIERRDPSRPRFVAGSIGPTNKTASISPDVNDPGYRNVSFDELAD
                     AYADAARGLVDGGADLLLIETIFDTLNAKAAIFGIKRYFDEAGIERPLMISGTIIDNS
                     GRNLSGQTAEAIYYSLMHAEPLIFGLNCSLGVEAFRDNMHAISRVANCYTSIYPNAGL
                     PNEFGEYDDTPEFMARVLREYAEAGLVNVVGGCCGTTPAHIRAFAEAVRGVAPRVIPD
                     VPAYARLSGMEPLVISPETNFVNVGERTNVTGSRRFAKLILNGDYATALDVARQQVEN
                     GAQMIDINMDEGMLDAEAAMERFLKLVAGEPDIARVPVMIDSSRWNAIEAALKCVQGK
                     SVVNSISLKEGDAEFIRQAKLARRYGAAVVVMAFDENGQAETADQRVAIAHRAYDILH
                     DQIGFPANDIIFDPNIFAIATGIEEHNNYAVDYFEAARRIKAELPGTMVSGGVSNMSF
                     SFRGNDPVREAMHSVFLYHAIQAGMDMGIVNAGQLAIYEDLDKDLLERVEDVVLNRRP
                     DATERLVTFAETVKGGGKERVEDLSWRELPVEDRLAHALVKGINSYVEADTEEARQQA
                     TRPLDVIEGPLMRGMSIVGDLFGSGKMFLPQVVKSARVMKQAVAYLIPYIEAEKAISG
                     DHRPAGKILLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMAPTQKILDTARREKVD
                     IIGLSGLITPSLEEMRHVAREMERQEFDIPLMIGGATTSKVHTAIKIAPGYTHGPVVH
                     VVDASRAVGVASQLLSDEQRDQFAADIAAEYAHLRETREGHAGQKPLATVAEARANRL
                     RLDWANYTPPVPNRLGITVFDDIDLNEVRQTIDWTPFFTAWELHGKFPRILDDPVVGE
                     SASALYTDAQAMLDRIVQEKWLTARAVVGLFPAVNVGDDILVFDPAGEKGRKGEGAQG
                     RLTTDLLNTDPLTTVHFLRQQMQRPPGRPNLSLADYIAPSDSGLTDYMGMFAVTAGIG
                     LDKVVARYEADHDDYNAILAKALADRLAESLAEYMHRCVRRELWGYAPDEDLDNEALI
                     AEAYRGIRPAPGYPACPDHTEKGELFRVLDVTAAIGLNLTESYAMVPTAAVSGYYIAH
                     PESRYYGIGRIGRDQAEDYAARKGMGIETVEHWLASSLW"
     gene            complement(4667..5617)
                     /gene="cofD"
                     /locus_tag="PLC06_11010"
     CDS             complement(4667..5617)
                     /gene="cofD"
                     /locus_tag="PLC06_11010"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HRO91016.1"
                     /translation="MTPFDSSSLRVVCLAGGVGGAKLADGLARVLPPENLTVIVNTGD
                     DFRHLGLTVCPDLDTVMYTLGGVANPETGWGRAGESRRAMDEIGRLGGPDWFTLGDLD
                     LATHLTRAHWLDAGETLTAVTRRLCAGFGIDPTVLPMSDAPAPTLIETEPGEILPFQI
                     WFVRERWQPAVRRVLLPEDARATTAVIRALETADLVVIAPSNPFVSIDPILNVYPIRA
                     LIEDVPRAVVAVSPIVAGQAVKGPAAKMMAERGMDVSAAAVADYYGHLIDGFVYDTRD
                     EGMATEDGLALLCTDTMMHTAADRERLAREVLEFAEEWLK"
     gene            5668..>5925
                     /locus_tag="PLC06_11015"
     CDS             5668..>5925
                     /locus_tag="PLC06_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91017.1"
                     /translation="MKNATLLGFDGDGVGVFNLQSTERVARVGDEVVVSGGRIIEEID
                     PLLDSELLLLSSFAGGCKGPYWIVGIFWYWNNTPTVIHKECL"
BASE COUNT         1161 a         1806 c         1905 g         1053 t
ORIGIN      
        1 ccgcctgacg gtgatcggtc ggacgaaatc cttgtattca accgtggctc tctgtcgacg
       61 ccgtttgagg tgacgaaagc catgagcgga tttatcgagg acaagccccc gacgggcccg
      121 gccaacgcca actgggtcac cggacagtat gccgggttcg ccgacggcac gctctattat
      181 agagcgcgcg tcgtcggcat ccccgtggat cagcccggca tgaagttcgg cttctgcttc
      241 tgggagaacg gctacgtcaa tgaggagtgt cgtgggcagg aattcgacgc catccccggc
      301 acggaaatga cctggtcgca tgcgctgagt gatatgtggg taaagtctcc gatcaactgg
      361 gctaacccgc gtaccaagca tggcatcgtc gtccgtaacc agaataacaa gccggtcagc
      421 gccaagaagg gctggaactg gtatggcgaa aatcctgacc attggtaccc gatgtatatc
      481 cattacacag ttgttcttgt caaggatggc ggtaccttta gcggctggga ttattacggc
      541 tggtagttaa tcgccgcgtt tcgaatctga tgtaaaacat gacaggtttc tgaaatctgt
      601 catgtttttt attgtggttg acacatcccc tttaattgac taacctttag gtaattgttc
      661 agataacaac cgtatactca tcgagagagg tggagggacc ggccctgcga tacctcggca
      721 accgactgaa tactcaggaa ggtgccaaat ccggcagaga tcgacggcag gtcgatcatc
      781 tggaagatga gagagactta gattaaccga gcctctcatc atgagaggct ttttcgttat
      841 aaccatgaac cgaaccgaac aactacacca gcaattggcc cagcgcatcc tcgtcctcga
      901 cggcgcgatg ggcacgatga ttcagcagta tcacttgagc gaggccgact accgcggcgc
      961 tcgctttgcc gactggccgc gcgatctgca gggcaacaac gacctgctcg tcctgaccca
     1021 gcccgacatc atccgcgcca tccatctggc ctatctggag gccggggccg acctgattga
     1081 gaccgatacg ttcaacgcca acggcatctc ccagggcgac tacggcatgg aggagctggc
     1141 ctacgagatg aacgtggccg cggcccggct ggcctgcgag gcgcgggacg agatcgaacg
     1201 ccgcgatccc tcgcggccgc gttttgtggc cgggtcaatc ggccccacga acaagacggc
     1261 atctatctcc cccgacgtca acgatcccgg ctatcgcaac gtcagcttcg atgaactggc
     1321 cgatgcctac gccgatgccg cccgcgggct tgtggatggt ggcgccgatc ttctgctcat
     1381 cgagacgatc ttcgacacgc tcaacgccaa ggcggccatc ttcggcatca agcgctactt
     1441 cgacgaagcg ggcatcgagc ggccgttgat gatctccggc acgattatcg acaacagcgg
     1501 ccgaaatctg tcgggccaga cggccgaggc gatctactat tcgctgatgc acgccgagcc
     1561 gcttatcttc ggcctcaact gttcgctggg cgtcgaggcg ttccgcgaca acatgcacgc
     1621 catctcgcgg gtggcgaatt gctacaccag catctacccc aacgccgggt tacccaacga
     1681 gttcggcgaa tacgacgaca cgccggagtt catggcccgc gtgctgcgtg agtacgccga
     1741 ggcaggactg gtcaacgtcg tcggcggctg ctgcggcacg accccggctc acatccgcgc
     1801 cttcgccgag gcggtgcggg gcgtggctcc gcgagttatc cccgacgtgc cggcctacgc
     1861 tcgcctgagc ggcatggagc cgctggtcat cagcccggag accaacttcg tcaacgtcgg
     1921 cgagcgcacc aacgtcaccg gttcgcgccg cttcgccaag ctgatcctca atggggacta
     1981 tgccaccgcg ctcgatgtgg cccgccagca agtggagaac ggggcgcaga tgatcgacat
     2041 caacatggac gaggggatgc tcgatgccga ggcggctatg gagcgcttcc tgaagctggt
     2101 ggcgggcgaa cctgacattg ctcgcgtgcc ggtgatgatc gactcgtccc ggtggaacgc
     2161 cattgaggct gcgctgaagt gcgtccaggg caagagcgtg gtcaactcga tcagcctgaa
     2221 ggaaggcgat gctgagttca ttcgccaggc gaagctggcc cgacgctatg gcgcggccgt
     2281 cgtcgtcatg gccttcgacg agaacgggca agcggaaacg gccgaccagc gcgtggccat
     2341 cgcccaccgc gcctatgata ttctccacga ccagatcggc ttcccggcca acgacatcat
     2401 ttttgatccc aatatcttcg ctatcgccac cggcatcgaa gagcataaca attacgccgt
     2461 ggactatttc gaggccgccc gccgcatcaa ggccgaacta ccgggcacga tggtcagcgg
     2521 cggcgtcagc aacatgagct tttccttccg cggcaacgac cccgtgcgcg aggcgatgca
     2581 ctccgtcttt ctctaccacg ccatccaggc gggcatggac atgggcatcg tcaacgccgg
     2641 gcagctggcc atttacgaag acctggacaa ggatctgctg gagcgcgtcg aggacgtcgt
     2701 gctgaatcgg cgacccgacg ccaccgagcg actggtcact ttcgccgaga cggtgaaggg
     2761 tgggggcaag gaacgtgtcg aggatttgtc gtggcgcgag ctgccggtcg aggatcggct
     2821 ggcccacgcg ctggtgaagg gcatcaactc ctacgtggaa gccgacaccg aagaagcgcg
     2881 gcagcaggcg acgcggccgc tggacgtcat tgaggggccg ctgatgcgcg gtatgagcat
     2941 cgtcggcgat ctgttcggca gcggcaagat gttcctgccg caggtggtga agagcgcccg
     3001 cgtcatgaag caggccgtgg cctatctgat cccctatatc gaggccgaga aggccatcag
     3061 cggcgaccac cgcccggcag gcaagatcct gctggcgacg gtcaaggggg atgtccacga
     3121 catcggcaag aacatcgtcg gcgtcgtgct gggctgcaac aactatgaag tcatcgatct
     3181 gggcgtgatg gctccgacgc agaagattct ggacacggcc cggcgcgaga aggttgacat
     3241 catcggcctc agcgggctga tcaccccgtc gctggaagag atgcgccacg tggcccgcga
     3301 gatggagcgt caggagtttg atattccgct catgatcggc ggggcaacca catcaaaggt
     3361 gcacacggcg atcaagatcg ctcccggcta tacccacggg ccggttgtgc atgtggttga
     3421 cgcctcgcgc gcggtcggcg tggcctcgca gctattgagc gacgagcagc gcgatcaatt
     3481 cgcggccgac atcgccgccg aatacgccca tctccgcgag acgcgcgagg ggcacgccgg
     3541 gcaaaagccg ctggcgacag tagccgaagc acgcgccaat cgtctgcgac tggactgggc
     3601 taactacacg ccgcccgtgc ctaatcggct ggggatcacc gtcttcgacg acatcgacct
     3661 gaacgaggtt cgccaaacga tcgactggac gccgttcttc accgcctggg aactgcacgg
     3721 caaattcccg cgcatccttg atgatcctgt cgtgggcgag agcgcgagcg cgctctacac
     3781 cgacgcgcag gcaatgctcg accggatcgt gcaggagaag tggctgacgg cgcgggcggt
     3841 ggtggggttg ttcccggcgg tgaacgtggg ggatgatatc cttgtgtttg atccggcggg
     3901 ggagaaggga agaaagggag aaggggcgca ggggcgattg accactgacc tactgaatac
     3961 tgatcctctg accacggttc actttttgcg tcagcagatg cagcggccgc ccggccgtcc
     4021 caacttgtcg ctggccgatt acatcgcccc atccgacagc gggctgaccg actacatggg
     4081 catgttcgcc gtcacggcgg ggatcggcct ggacaaggtt gtggcccgct acgaggccga
     4141 ccacgacgac tacaacgcca ttttggccaa ggcgctggcc gaccggctgg ccgaatcgct
     4201 ggcggagtac atgcatcgct gcgtgcgacg cgagctatgg ggctatgcgc cggacgagga
     4261 cctggataac gaggcgctca tcgccgaggc ctatcgcggc attcgccccg caccaggcta
     4321 tccggcttgc cccgaccaca cggagaaggg ggagctgttc cgcgtgctcg atgtcacggc
     4381 ggctatcggt ctgaacctga cggaaagtta cgccatggtg cccacggcgg ccgtcagcgg
     4441 ctactacatc gcccaccccg aatcacgcta ctacggcatc ggccgcatcg gccgcgacca
     4501 ggcagaggat tacgccgcgc gcaaggggat gggcatcgaa accgtggagc attggctggc
     4561 gtcgtcgttg tggtaacaga gggcagcagg ggtgagtaga ttcgctaacc gcgccgggac
     4621 gaaatagtaa atgaggtacg actctcgtgt gagggtcgta cctcatttat ttcaaccatt
     4681 cctcggcaaa ctccaacacc tctcgcgcca gtcgctcccg gtcggcggcc gtgtgcatca
     4741 tcgtatccgt gcacagcagc gccagcccat cctcggtggc catgccttcg tcgcgcgtgt
     4801 cgtagacgaa gccgtcgatg agatggccgt aatagtcggc aacggccgcg gccgacacgt
     4861 ccatcccgcg ctcggccatc atcttggccg ccgggccttt caccgcctgc ccggcgacga
     4921 tgggcgagac ggccaccacc gcccgcggca cgtcctcgat cagcgcccgg atggggtaga
     4981 cgttgaggat cgggtcgatg ctgacgaacg ggttggaggg ggcaataacg accagatcgg
     5041 ccgtctccag ggcgcgaatc acggccgtcg tggcccgcgc atcctcaggc agcagcacgc
     5101 gccgcacggc cggctgccac cgctcgcgga cgaaccatat ctggaagggc agtatttcgc
     5161 cgggttccgt ctcgatgagc gtcggcgcgg gagcgtcgct catgggcagc acggttgggt
     5221 cgatgccgaa cccggcgcac aggcggcgag tgacggccgt cagcgtctcg cccgcgtcca
     5281 gccagtgggc gcgggtcagg tgggtggcca ggtcgaggtc gcccaacgtg aaccagtccg
     5341 ggccgccgag gcggccgatc tcgtccatcg cgcgccggct ctccccggcg cggccccagc
     5401 ccgtctccgg attagccacg ccgcccagcg tgtacatgac cgtgtccagg tcggggcaga
     5461 cggtcaggcc caggtggcga aagtcgtcgc cggtgttgac gatgacggtc agattttccg
     5521 gcggcaggac gcgagccagg ccgtcggcca gcttggcccc gccgacgccg ccggcgaggc
     5581 agacgacgcg aagcgaagaa gaatcgaagg gtgtcatgtt ataattatcc gtcaggtaga
     5641 gaattgcgcc attccggagt gtcgaggatg aagaacgcta cgctgcttgg gtttgacggc
     5701 gacggggtgg gagtatttaa cctgcaaagc acggagcgcg tggcgcgcgt gggtgacgag
     5761 gtggtggtca gcggcggccg catcatcgag gaaatcgatc cgctgctgga cagcgaactg
     5821 ctgctcctgt cgtcgtttgc cggtggatgt aaagggcctt actggattgt ggggatattt
     5881 tggtactgga ataacacacc gactgtcatc cataaggagt gtctt
//
LOCUS       DAOMQG010000202         5925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5678_length_5925_cov_6.163203, whole genome shotgun sequence.
ACCESSION   DAOMQG010000202 DAOMQG010000000
VERSION     DAOMQG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_5925_cov_6.163203"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="PLC06_11020"
     CDS             complement(<1..1963)
                     /locus_tag="PLC06_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91018.1"
                     /translation="MGNESHTAHTAHTFSDLLSDALDARGVSPGEAARALKGMGLKID
                     RGTLTRWRNGETTPSLDKLDVVRRLPDALGMTSAERAAFLREAGRLLDFALVRERRPR
                     PVAAVVLPQRIHFGADVLPPFAGRVAELALLQECVRDGRSVLITGLGGVGKTRLAQEL
                     LRSSVGYFAHGCEFLTLLPGQPGDQVLYNVAQLLGVPLPSGIPENGRALVLAGLRDQL
                     RDARLLFLIDNVENADQVRDLIRELPSITWVITARRISLKSIGVYPVVLDLPPTEDAA
                     AIFLAHAGGVHASGGEGSALVDEAIHVAGRLPIALRLMAGLMSSSFINSPAELEAWIQ
                     AGGLLRGGSHGMTLRRLFAQLVDSAPAEAQAIFEACGAFADRTIAMQRLLSVCLRAGI
                     RPSPRIWEWLADLSLIELPDEGYVQLHPLLHTYAAARLRSSPRAAAIREGFRGVYLDL
                     ARAAAESAEPQRDYAALIPEEPNLLRVVDEYYKEGDWAGLRAMWPAVSGYLWHMGNLA
                     AYEALDRKCLRAAWLSGDSAWVAVLLSELGFVALEMGRFDGAAELFRDSQAIHDAAPE
                     QLIEQARLRRYRAALAMRLGRLDEALALLAECEQRLSTLTDPPESRLGIARVLLHSAR
                     MSAHRRRGDLPSAAAAGELADRQFRAISPN"
     gene            2376..3080
                     /locus_tag="PLC06_11025"
     CDS             2376..3080
                     /locus_tag="PLC06_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91019.1"
                     /translation="MGKDVPAAVALEEPAFLYDDDLQADAVGQKIQDEAGIAAYYKAP
                     NALNLEFVRPLFNSIELESADYILGSISVPSYAEHFDTHVYIRRDGWIMAYYLSSAPA
                     SKMIETKAGSLNNSKLTIVMNNIAVTGGAPFGAVTYYNFNHPNATHMLLVGESDYEEF
                     QITIPSVYSYYEISWASKTHEIRIDGQDLTNIPHGYGLIPITSFLPDITHTIHTGYDW
                     NNGIEYGALVIVYRMP"
     gene            3077..3742
                     /locus_tag="PLC06_11030"
     CDS             3077..3742
                     /locus_tag="PLC06_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91020.1"
                     /translation="MTTRHYITAIFVAAFMVGLLWGGRARGLAENFLIEGADETRYES
                     LTQSFTLNSLLDALAPHFVVDMADSLRHAPLIYPSGLQAYFDALPAHFVLDMADSNRF
                     SSLQYPLALIGDTTPPKEEGTPEIVPTGSGSVKIRWKTNEFSRGTIEFGPQPGQYNRT
                     VSESLFARDHELTLAGLPSKGTIYYRIANVDPAGNNGRGAERSFAFGVEVNKLFLPIT
                     LRR"
     gene            3847..5214
                     /locus_tag="PLC06_11035"
     CDS             3847..5214
                     /locus_tag="PLC06_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HRO91021.1"
                     /translation="MTLKSQRSIAVLIVVILGICLFGSGIVAAQSNSPAPTPAPGGEN
                     LPSQAPGEDSVLPLGKTDKDDRRLIANGKSLTGKISPSTDEDTYIFYATEGQQVTINM
                     TAKTTALDGYLKLYTARDEQLRAYDDDSGGNRNPMLYMYTIPRSGKYLIVLSSYGGYS
                     SGTYTVRLDVYGGDSDDNRVLESGKTASGKIIPAYDIDSYFFQGVEGTRATIRMDKKG
                     GSLDSYLYLYDPDNVLIAANDDSGGNGNALIDWVTLPTSGAYRVEARSYAGNSSGAYK
                     IRLDQTKPNLALNKPSTAWNWHAPYYLPEYGNDGNLNTRWSGDYGTNWYWIDLGSSMT
                     FNQVKINWEAAYATSYFIGWSDAPNCIGTYTGNNYTANSIGYKDHNLGKRTARCVAIR
                     MDTALSWATNYSFWEFEVYNLAASNSASPPGSIPGDAVLVDVVPEADFSEEELIQMDL
                     LSPTN"
     gene            complement(5369..>5925)
                     /locus_tag="PLC06_11040"
     CDS             complement(5369..>5925)
                     /locus_tag="PLC06_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91022.1"
                     /translation="AGVTVDDSDIIRFDPVTGQFSWYFDGSDVGLTTGGENVDAIALL
                     PDGRLLISTSGAASVPGLKAADEDVMVFDGAFGSDTTTRTWALYLDGSDLSKKLSDID
                     GLAWLPGGGAGLGVLYLSADKKVTLGGQEMAPGDVFFCEIATLEATSSCATIGRYWQG
                     TAAGLPVKADVDAVEVGVEPPLGE"
BASE COUNT         1260 a         1828 c         1749 g         1088 t
ORIGIN      
        1 cgttgggaga gatggcccgg aactgccggt cggccagctc tccggcggcg gcggccgacg
       61 gcaaatcccc ccggcgacgg tgggcgctca tccgcgcgct gtggagcagc acccgcgcga
      121 tgcccagccg cgactccggc gggtcagtca gcgtcgacag gcgctgctcg cactccgcca
      181 gcagcgccaa cgcctcatcg agacggccga gacgcatggc caacgccgcc cgatagcgcc
      241 gcagccgggc ctgttcgatc aactgctccg gtgcggcgtc gtggatggcc tgcgaatcgc
      301 ggaacagttc ggccgcgccg tcgaaccgcc ccatctccag ggcgacaaag cccagttccg
      361 acagcagcac cgccacccag gccgaatcgc ccgacagcca cgccgcccgc aggcacttcc
      421 gatccagcgc ctcataggcg gccagattgc ccatgtgcca caaataaccg gagacagccg
      481 gccacatggc ccgcagcccc gcccagtcgc cttccttata gtattcatcg accacgcgca
      541 gcagattcgg ctcttcgggg atgagcgccg cataatcgcg ttggggttcg gccgattcgg
      601 ccgcggcgcg agccaggtcc agatagacgc cgcggaaacc ctcgcgtatc gccgccgcgc
      661 gggggctgct cctgaggcgc gcggcggcgt aggtatggag cagcgggtgg agctgcacat
      721 agccctcatc gggcagttcg atgagcgaca gatcggccag ccactcccaa atccggggcg
      781 agggccggat gccggcgcga aggcaaaccg acagaagccg ctgcatggcg atggtgcgat
      841 cggcaaacgc tccgcaagcc tcgaagatgg cctgggcctc ggccggagcg ctgtcgacga
      901 gttgggcgaa aagccgccgc agggtcatgc catggctgcc gccgcgcagc aggccgcccg
      961 cctgaatcca ggcttccagc tcggccgggg agttgataaa gctggaggac atcagcccgg
     1021 ccatgagccg cagcgcgatg ggcagccgcc cggcgacgtg gatcgcctcg tccaccagcg
     1081 ccgatccctc gccccccgac gcatggacgc cgcccgcatg ggccaggaag atggccgcgg
     1141 cgtcctcggt cgggggcagg tcgagcacca cgggatagac gccgatgctc ttcaggctga
     1201 tgcgccgggc ggtgatgacc caggtgatcg acggcagctc gcggatcagg tcgcgcacct
     1261 ggtcggcgtt ttccacgttg tcgatgagga acagcaggcg ggcgtcgcgc agctggtcgc
     1321 gcagaccggc cagcacgagg gcgcggccgt tttcgggtat gcccgacggc agcggcacac
     1381 ccaacagctg ggcgacgttg tagagcacct ggtcgccggg ctggccgggc aagagggtga
     1441 ggaactcgca gccgtgggcg aaatagccga ccgaggagcg cagcagctcc tgggccagcc
     1501 gggtcttgcc cacgccgccc agcccggtga tgagcaccga tcggccgtcg cggacgcact
     1561 cctgaagcaa agccaactcg gccacgcgcc cggcgaaggg gggcagcacg tcggctccaa
     1621 aatggatgcg ttggggcaag accacggcgg cgacgggccg cggccgccgc tcgcgcacca
     1681 gggcgaaatc gagcagccgc ccggcctcgc ggagaaacgc cgcccgctcg gccgaagtca
     1741 tgcccagcgc gtccggcagc cggcgaacga catcgagctt gtcgaggctg ggggtcgtct
     1801 cgccgttgcg ccagcgggtc agcgtgccgc ggtcgatctt cagccccatc cccttgagcg
     1861 ctcgcgccgc ctcgcccggc gacacgccgc gcgcgtcaag cgcatcgctg agcaggtcgg
     1921 aaaaggtgtg ggccgtgtgg gccgtgtggc tctcgttccc catggtgtgt gtgaaaggtg
     1981 ttgcacgggt gcaacaagtt tcattctaaa ctaacggtta tggctgactt tcggttggcc
     2041 gggggtgttc cagtcgcgat gtcaatgtag cccccacacg aatcgataat cacataattc
     2101 ctccatgcac gcggctttgc gcttcatcat cgagctacag gctctcgggg ggtaatcagc
     2161 atacaacgag cgagatcagg cggctgatgg atgactcgat tctagtttaa cgcagggaat
     2221 ttggcaaacg tgggggttgg aaattcgggc cggcaaagcg accattatcg aaggagggat
     2281 acgtgcttcg atccatatct catacgttca gagggctaac aatcggtttt tttgtcgtct
     2341 tattgggcgt cgccggcgtg gcggcgggaa ggcacatggg caaggacgta cccgcggccg
     2401 ttgccctgga agagccggcg ttcctgtatg acgacgacct gcaagccgat gccgtgggtc
     2461 agaaaattca ggatgaggcg ggtattgccg cctattacaa ggcgcccaat gcgctaaatc
     2521 ttgaattcgt ccgccccctc ttcaacagca tcgaactgga atcggccgac tatattcttg
     2581 gatcgatctc cgttcctagc tacgccgaac acttcgacac tcatgtctac atccggcgtg
     2641 acggttggat catggcctac tatctgtcat ctgcgcccgc gagcaagatg atcgagacga
     2701 aagccggaag tctcaacaat tccaagctaa cgattgtaat gaacaacatt gctgtcactg
     2761 gcggtgcacc ttttggcgcg gtcacgtatt acaacttcaa tcatcccaat gcgacgcaca
     2821 tgctcttagt gggcgagtca gactatgagg aatttcaaat aaccattcca tctgtctatt
     2881 catattacga aataagttgg gcgagtaaaa cgcatgagat aaggattgac ggccaagatc
     2941 tcactaacat tccccatggt tacggtctaa ttccaatcac atcgttttta cctgatatca
     3001 ctcacacaat acacacaggc tacgattgga acaatggtat cgagtatggc gcgttagtaa
     3061 ttgtttatag gatgccatga ccacccgaca ctacatcact gcaatattcg tcgcagcctt
     3121 tatggtcgga ctactgtggg gcggccgagc acgtggactt gcggaaaact tcctcatcga
     3181 gggggctgat gaaacccgct acgaatccct aacccagtcc tttacgttaa acagcttgct
     3241 ggacgcgctg gccccgcact tcgtggtcga tatggccgac agcctgcgcc acgcgccgct
     3301 gatctatccg tcgggattgc aagcgtattt cgatgccttg ccggcgcact ttgtgctcga
     3361 tatggccgac agcaatcgct tctcatccct gcaatacccc cttgcgctga tcggcgacac
     3421 cacgccgccc aaagaagagg gcacaccgga gatcgtcccg accggctccg gcagcgtcaa
     3481 gatccgctgg aagacgaacg aattcagtcg cggaaccatc gaattcggcc cgcagccggg
     3541 gcaatacaac cggacggtct ccgagtcgtt gtttgccagg gaccatgaac tgaccctggc
     3601 cgggctgccg agcaagggca ctatttacta tcggatcgcc aacgtcgacc cggccggcaa
     3661 taacgggcgc ggagcggagc gaagtttcgc atttggggtg gaggtcaata agctcttcct
     3721 gccgatcaca ttacgcaggt aagtttgatt aaggtgtcgt tggggcgact cggttcgatt
     3781 gagtaatcaa gagatttcga ttactgcccc ttaatagtcc ccattcagag catcacagga
     3841 ggaatcatga cgctcaaaag tcaacgtagt attgcggtat tgatcgtggt aattctcgga
     3901 atctgcttgt tcgggtcagg cattgtggcg gcacaatcca acagtcctgc acctacaccg
     3961 gcacccggag gagaaaatct gccgtcgcag gctccgggag aggatagcgt tctgccgctc
     4021 ggaaaaaccg acaaggatga cagacgactc attgcaaatg ggaaatcact gacagggaaa
     4081 attagccctt ctacggatga ggatacgtac atcttctacg cgaccgaggg ccagcaggtc
     4141 actatcaata tgaccgcaaa aaccaccgcg cttgatggct acctgaagct ttatacggca
     4201 agggatgagc agttgcgggc atacgatgat gatagtggcg gtaatcgcaa tccgatgctc
     4261 tatatgtaca cgataccgcg atcaggaaaa tatctgatcg tgctcagcag ctatggcggc
     4321 tactcctccg gcacctacac agttcggctg gacgtgtatg gcggcgattc cgatgacaat
     4381 cgggtgttgg agtcaggcaa aacagcctcc ggaaaaatta ttcctgccta tgacattgat
     4441 agttattttt tccagggcgt tgaaggtaca agagccacaa ttcgcatgga caagaaaggc
     4501 ggctcgctgg atagctacct gtatctctac gacccggata acgtgctcat tgccgcgaat
     4561 gatgacagtg gcgggaatgg caatgcgctc attgattggg tcaccttacc aacatccggc
     4621 gcgtatcgcg ttgaggccag gagttacgct ggtaactcaa gtggggcata caaaatacga
     4681 ctcgaccaga cgaagccgaa cctggcgctg aacaagccca gtacggcttg gaactggcac
     4741 gcgccgtatt atctgcccga atacggcaat gacggcaatt tgaacacgcg ctggtcgggc
     4801 gattatggca ccaattggta ctggattgat ctcggcagta gcatgacgtt taaccaggtc
     4861 aagatcaact gggaagcagc ctatgccacc agctatttta tcggctggtc agacgcaccg
     4921 aattgtatag gcacctatac cgggaacaac tatacggcca acagcatcgg ctacaaggac
     4981 cataatctgg gcaagcgaac ggcccgctgc gtcgccattc gcatggacac agccctctca
     5041 tgggcaacga actattcctt ctgggaattt gaggtctata accttgccgc cagtaattca
     5101 gcgtcgcctc cgggatcgat accgggggat gccgtgctgg tcgacgtcgt gcccgaggcc
     5161 gacttcagcg aagaagagct tatccagatg gatctgctta gtcccaccaa ctagagcgca
     5221 ttctgtctcg cctctgccag accctcaagc caaatctcga cggcctggac atagcatcgt
     5281 aggacaacag cagtaaccta aacccaaaaa gaccccggcg gtcccaacaa accgccgggg
     5341 tcttttttct tatccgaccc gcctgaacct actcccccag cggaggctcc acccccacct
     5401 ccaccgcatc cacatcagcc ttaaccggca gccccgcggc cgtgccttgc caatagcggc
     5461 cgattgtggc gcagctcgat gtcgcttcca gggtggcgat ctcgcagaag aacacgtcgc
     5521 cgggggccat ctcctgcccg cccagcgtca ccttcttgtc ggctgacagg tagagcacgc
     5581 ccagccccgc cccgccgccg ggcagccagg ccagcccgtc gatgtcgctc aacttcttcg
     5641 acagatcgct gccgtccaga tagagcgccc atgtcctcgt cgtcgtgtcc gaaccgaacg
     5701 cgccgtcgaa gaccatcacg tcctcgtcgg ccgcctttag gcccggcacc gacgccgcgc
     5761 ccgacgtgga gatcagcagc cgcccgtcgg gcagcagggc gatggcgtcc acgttctcgc
     5821 cgccggtcgt cagacccacg tcggagccgt cgaaatacca gctgaactga cccgtgaccg
     5881 gatcgaagcg gatgatgtcg ctgtcgtcga ccgtgacccc cgcca
//
LOCUS       DAOMQG010000203         5919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5685_length_5919_cov_7.828104, whole genome shotgun sequence.
ACCESSION   DAOMQG010000203 DAOMQG010000000
VERSION     DAOMQG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_5919_cov_7.828104"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLC06_11045"
     CDS             <1..1252
                     /locus_tag="PLC06_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRO91023.1"
                     /translation="QFTPGLGTLLLAIILLGAVGSLLHDARDVSLGIGGFLWGGAVLI
                     SFILGTAYISRRLLPIGGNQGWSRGFQLLWRNYARGVGDLLSGAQSEPPIPASKRKKI
                     RADELSPSFKSLKAGFLYSHQAVAIARGNGYSRADGPGLVFLNSGESIAKVIDLRPQS
                     RRQSVSAMTRDGIPVETGVSVTFRVRRPTAGHRRPRSVENDPIPYPYDKEALFDLTYA
                     AGTIGESKRSWTELVAPQAATMLVTEIGKYTLDELLVGAGAEPLSEIRERIKSGLAEQ
                     QTAGDYQTISRGIDILGVGVGSLELPADVIAKRLTTWQVTWKNRISEETVSGDIDAQR
                     LYAQARARAQVENIENLLTSVEALRNTSGIDLHEVVMLRLMEILESVSANRSLMPIAS
                     RAALNSLASEASSEIRQALEQDEA"
     gene            1257..2666
                     /locus_tag="PLC06_11050"
     CDS             1257..2666
                     /locus_tag="PLC06_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRO91024.1"
                     /translation="MAGQTQPRRASRPTAPRLSFPGLLVINWPWFLLAALLVGYWWYA
                     ASLERVDANLLLARIFLPDLPPSQAAEATFSPWITLLVEMFHPRVLRHLIPIVVGWWL
                     AVQAAISLMQVLYDCPDRRTAAEFLRRQRRDSVNPLDIHEISAENLHEEREKSILLGV
                     GGPVRVQIPDGYAAVTERNARTLRTLPPGTHNLGRFEYVQGLVDLQPLDKSAKDVPML
                     TREGIPVKVNIGLAFRIDPGDLPVTHSRPFPFREEAVRQAVYSGAVGANGVVSSWEDG
                     PLGKVRGALSGMVAEDSLDELLAAESPRDAHHLLTSSVMRKVWDGLPKEGIKPLRMQI
                     GRLTPPPEVLQQYTEFWLANKRKEDMLARADRLAPLVEEAETARVAAEIAMINAVAEG
                     IRRAQQEAGSYLPGYLLALRLMEALRRMFQHSADDLESVGGDTLQLLDEMGNVEERLE
                     NLQNQLQLPQPKFRPSSPD"
     gene            2694..3875
                     /locus_tag="PLC06_11055"
     CDS             2694..3875
                     /locus_tag="PLC06_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91025.1"
                     /translation="MVAQAPHPSDSYRSASDAEPLRPVREAVVVLEGLLKILFPDPLS
                     LDIITVENIVQRYPLTGLGAPALAAIDQSQRIVRARGDYGQSGLAEFHIGLIYLHWDD
                     CRAAANQFALARQPWSLAGDAPAMCLAHYAQGLGLYHSYHNEAAMLQFSRAERLLNRP
                     ATGTQGERFAALAKEMRRVLLIAQETLRDSLWPKEQQSAEVLQAGYLSVPPNMAPETP
                     PAAQAATEPDPRHALRRRAERPEWAKRVPPPFSNLPDGIGDTPRGPLPGHITTDERFV
                     WYQIADRKGEFLPNVSSGMWVLADREVDERGPVGREYVVVGGAQDELGSIAVRPIAHG
                     SAMPYCYLGYRVADEAAPEQSRIFLDNDEQPSAPDEVLVLAVVEGFWYGLNSRALPGS
                     D"
     gene            3940..4869
                     /locus_tag="PLC06_11060"
     CDS             3940..4869
                     /locus_tag="PLC06_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRO91026.1"
                     /translation="MAKHVLVIGATLLDTKGKPVAGLAPGTSNPSEIRSMRGGTARNV
                     AENLARLGAEVVLISAVGDDPTGRQLLIQTAESGVNLDYVEMVPDQATGSYIALLEAD
                     GLLSVALDDVRVMETITPDYLNRNRALFRDAAMIMFDGSLTVAAMRTVVRLAREYGVF
                     LCADPSSARLAYKLRPFLADIDLVVPNQVEEAELCEMDFAGYDPTAGLIMARRLLGSG
                     VGTVVVTLSDFGLDYATADEMGYIPPSHSKFVDSTGTGDAVTAAVMFGLLNDLPIVEA
                     IRLGAAAAAITLQTNETVVPDLSLDMLYEHLIV"
     gene            4917..5582
                     /locus_tag="PLC06_11065"
     CDS             4917..5582
                     /locus_tag="PLC06_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91027.1"
                     /translation="MSNTLGIVICFAYIFVVIGLAEGLRRWRGYGSGFTRKIIHIGVG
                     MMSWFIPFLFTSAWPFVLACAAFMVINLIDWRYGLIGSMQSKHRSNLGTVYFPFAAAV
                     VALIFWEQPPLMVAALMPLTWGDGMAPVIGEKYGHRFYQVYTSRRTLEGSLAFFVAGF
                     VFTWLALWLMPGLPAITPAAAILPALVVMAVTTLVEAVSIWGLDNLAVTAVAIVILGL
                     WPF"
     gene            complement(5618..>5919)
                     /locus_tag="PLC06_11070"
     CDS             complement(5618..>5919)
                     /locus_tag="PLC06_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRO91028.1"
                     /translation="TRERLARLGYNNVWVHHGDGTRGWPEHAPYDGIIVAAGGPTVPE
                     SLRRQLAAGGRMVIPVGQNRHQQNLILITRPTADTWAEKMLAPVAFVPLIGDEGW"
BASE COUNT         1021 a         1806 c         1938 g         1154 t
ORIGIN      
        1 acagttcact cccggcctgg gaactctcct gctcgcgatc attcttctgg gcgcggttgg
       61 atcgttgttg catgatgcca gggatgtatc gttagggatt ggcggctttt tgtggggcgg
      121 cgcggtgctg atcagcttca tcctcggcac agcctatatc tcgcgccggc tgctgccaat
      181 cgggggcaat cagggctggt cgagggggtt tcagttgcta tggcgcaact atgccagggg
      241 ggtgggcgat cttctctccg gcgcccaatc cgaaccgccc attcctgcat ccaagcggaa
      301 gaagatcagg gccgacgagc tgtcgcccag cttcaaatcg ctcaaggcag gctttctcta
      361 tagccaccag gcggtagcca ttgcccgcgg caatgggtat tcacgcgccg acggcccagg
      421 attggtcttc ctcaacagcg gagaatccat tgccaaagtg atcgatctgc ggccgcaatc
      481 gcgccggcaa tcggtcagcg ccatgactcg cgacggcatc cccgtggaga cgggcgtctc
      541 ggtgacgttc cgggtgcgtc gccccacggc cggccaccgc cggccgcgct cggtcgagaa
      601 cgaccccatt ccctatccct atgacaagga agccctgttc gacctgacct atgccgctgg
      661 aaccatcggc gagagcaagc gaagctggac ggagctggtg gccccgcaag ccgcgaccat
      721 gctggtcacc gagatcggca agtacacgct ggacgagctt ctcgtcgggg ccggcgccga
      781 gccgctgtcg gagatcaggg agcggatcaa gagcggcttg gcagagcagc aaacggccgg
      841 cgattaccag acgatctcgc gggggattga tatcctcggc gtgggagtcg gctcgctgga
      901 attgcccgcc gacgtcatcg ccaagcgact gacaacctgg caagtgacct ggaagaaccg
      961 catctccgaa gagacggtga gcggcgacat cgacgcccaa cgcctttatg cgcaggcgcg
     1021 cgcccgcgcc caggtcgaaa atatcgagaa cctgttgacc agcgtcgaag cgctgcgcaa
     1081 tacgagcggc atcgatctgc acgaggtggt gatgctgcga ctgatggaga ttttggaatc
     1141 cgtctcggcc aatcgctcgt tgatgcctat tgcttctcgt gcagcgctga actcgctggc
     1201 gtcggaggcg tcgagcgaaa tacgtcaggc gctggaacag gacgaagcct agcgccatgg
     1261 ccggacaaac ccaaccccgt cgcgcttctc ggccgactgc gccacgcctg tcattccctg
     1321 gtctgctcgt catcaactgg ccgtggttcc tgttggccgc gctgcttgtc ggctactggt
     1381 ggtatgcggc ctcgctggag cgggtcgatg ccaatttgct cctggcccgt atctttttgc
     1441 ccgatttgcc ccccagccag gcggccgagg cgactttctc gccctggatc acattgctgg
     1501 tcgagatgtt ccatccgcgg gtgttgcgtc atttaatccc gatcgtcgtc ggatggtggc
     1561 tggccgtgca ggcggcgatc agcctgatgc aggtgctgta tgattgcccc gaccgccgca
     1621 cggcggccga attcctgcga cgacaacgac gagacagcgt gaaccccctg gacatccacg
     1681 aaatatccgc cgaaaacctg cacgaagaac gggagaagtc gatcttgctg ggcgtcggtg
     1741 gcccggtgcg ggtgcaaata cccgatggct atgccgccgt caccgagcgc aacgcccgga
     1801 cgctgcgcac gctgccgccg gggacgcaca atctgggacg cttcgaatat gtccagggcc
     1861 tcgtcgatct gcagccgctg gataaatcag ccaaggatgt gccgatgttg acccgcgagg
     1921 gcatcccggt gaaggtcaat atcgggctgg cctttcgcat cgatcccggt gatttgcccg
     1981 tcactcacag ccgccctttc ccgttccgcg aagaagcggt gcgccaggcg gtctattcgg
     2041 gcgcggtggg ggccaatggc gtcgtctcgt cgtgggagga tggcccattg ggtaaggtgc
     2101 gcggcgcgct ttcgggcatg gtggccgagg attcgctcga tgagctgttg gcggccgaaa
     2161 gcccgcgcga cgcccatcat ctgctgacca gctccgtcat gcgcaaggtg tgggatggct
     2221 tgccgaagga aggcatcaag ccgttgcgga tgcaaatcgg ccgcctgacg ccgccgccgg
     2281 aggtgttgca gcaatacacg gagttctggc tggccaacaa gcgcaaggag gatatgctgg
     2341 cccgtgcgga caggctggcc ccgctggtcg aggaggcgga aaccgcccgc gttgctgccg
     2401 agatcgccat gatcaacgcc gtggccgagg gcattcgccg cgcccaacag gaagcaggct
     2461 cctatctgcc gggctatctg ctggcgttgc gcctgatgga agcgctgcgg cggatgttcc
     2521 agcattcggc cgatgacctg gagagcgtcg gcggcgacac cctgcaatta ttggacgaga
     2581 tgggcaatgt tgaggagcgg cttgagaatc tgcaaaacca gttacaattg ccacaaccaa
     2641 aattcaggcc gtcctcgccc gattaggcga gcggctacgc gagaccgaag atcatggtag
     2701 cccaggcacc ccatcccagc gactcatacc ggtcggcatc tgacgcggag ccgctgcgcc
     2761 ctgtgcgcga agcggtggtc gtgcttgagg ggctgctcaa aatactgttc cccgatccgt
     2821 tgtctctcga catcatcacc gtcgagaata ttgtccagcg ctatccgctg accgggttgg
     2881 gcgcgccggc gctggcggcc atcgatcaat cgcaacggat cgttcgggcg cgtggggatt
     2941 atgggcagtc ggggctggcc gagttccaca ttggcctgat ctatctgcat tgggatgact
     3001 gccgcgcggc cgccaatcag tttgccctgg cgcgccagcc gtggtcgttg gccggcgacg
     3061 ccccggccat gtgtctggcc cattatgccc agggactggg gctttatcat tcgtaccaca
     3121 acgaagcggc catgctccag tttagccgcg ccgagcgcct gctcaatcgg ccggccaccg
     3181 gcacgcaggg agaacgcttc gccgccctgg ccaaggagat gcgtcgcgtg ctgctgatcg
     3241 cccaggagac actgcgcgat agcctgtggc cgaaggagca gcaatcggcc gaagtattgc
     3301 aggccggtta tctctccgta ccgcccaaca tggcgcccga aacgcccccc gccgcgcaag
     3361 ccgcgaccga gcccgacccc cggcatgcgc tccgacgaag ggccgaacgg ccggagtggg
     3421 ccaaacgcgt gccgccgcct ttctcgaacc tgcccgacgg cattggcgac acgccgcgcg
     3481 gcccgctgcc gggccacatc acgaccgatg aacggttcgt ctggtatcag atcgccgaca
     3541 ggaaaggcga atttctgccc aacgtctcgt cgggcatgtg ggttttggcc gaccgcgagg
     3601 tggacgaacg cgggccggtt gggcgcgagt acgtcgtcgt gggtggcgcg caggatgaat
     3661 tgggcagcat cgccgtgcgg cccatcgccc atggcagtgc catgccttac tgctatctgg
     3721 gctatcgcgt cgccgacgaa gccgcgccgg agcaatcgcg gatatttctg gataatgacg
     3781 aacagccgtc ggccccggat gaggtgctgg tgttggccgt tgtcgaaggg ttctggtatg
     3841 gtctgaatag ccgcgccttg ccgggatctg attgagaata ctggaccgga gcgggagaaa
     3901 cccgccactt tgttatcgcc ggacgagaca gggggatgaa tggctaagca cgtgttggta
     3961 atcggagcca cattgctcga tacaaaaggc aaaccggtag ccgggttagc gccgggcacg
     4021 tcgaacccaa gcgagatccg gtcgatgcgg ggcggcacgg cgcgcaacgt ggccgagaat
     4081 ctggcgcggc tgggggctga ggtcgtgctg atctcggctg tcggcgacga ccccactggc
     4141 cgccagctgc tgatccagac ggccgaatcc ggtgtgaacc tcgattacgt ggagatggtg
     4201 cccgaccagg cgaccggctc ctatatcgcc ctgctggaag cggacggctt gttgtctgtc
     4261 gctctcgacg atgtgcgggt catggagacg atcacccccg attatctgaa tcgtaaccgg
     4321 gcgctcttcc gcgacgcggc gatgatcatg tttgatggca gtctgaccgt ggccgccatg
     4381 cgcaccgtcg tccggctggc gagggagtat ggcgtctttc tttgcgccga tccctcgtca
     4441 gcccggctgg cctataagct gcggccgttt ctggccgata tcgatctggt tgtgcccaat
     4501 caggtcgaag aagccgaact gtgcgagatg gatttcgccg gctatgaccc gacggccggc
     4561 ttgatcatgg ctcggcgact gctgggatcg ggcgtgggga cggtagtggt cactttgtcg
     4621 gactttggcc tcgattacgc cacggccgat gagatgggtt acatcccgcc atcccattcg
     4681 aaatttgtcg acagcaccgg cacgggcgac gcggtgacgg cggccgtcat gttcggcttg
     4741 ctcaacgatc tgcctatcgt ggaagctatc cgtctgggcg cggccgcggc ggcaatcacg
     4801 ctgcaaacca acgagacggt cgtgcccgac ctgagtcttg atatgttgta tgagcatttg
     4861 attgtctaga tgaccgccgg tcatctttaa cccgactcac ccggataccc atccccatga
     4921 gcaacaccct cggaatcgtc atctgctttg cctatatttt tgtcgtcatc ggcctggccg
     4981 agggactgcg ccggtggcgg ggctatggct ccggcttcac gcgcaagatc atccatatcg
     5041 gcgtggggat gatgagctgg tttatcccct ttctgttcac ctcggcctgg ccttttgttc
     5101 tggcctgcgc ggcattcatg gtcattaact tgatcgattg gcgctacggc ctgatcggtt
     5161 cgatgcagag caagcaccgc agcaacctgg gcacggtgta tttcccgttt gccgcggccg
     5221 tcgtggcgct gatattctgg gaacaaccgc cgctgatggt ggccgcgctg atgccgctga
     5281 cgtggggcga cggcatggct ccggtcatcg gcgagaagta tggccaccgc ttctatcagg
     5341 tctataccag ccggcggacg ctggaaggct cgctggcctt cttcgtggcc gggttcgtct
     5401 tcacctggct ggcgttgtgg ctgatgccgg gtctgccggc aatcacgccc gcggccgcga
     5461 ttttgccggc gctggtcgtc atggcggtga cgacgctggt agaggcggtg tcgatctggg
     5521 ggctggacaa cctggccgtg acggcggtgg cgatcgtcat cctcggcttg tggccgtttt
     5581 agcgcagaag ggctgctcta atttccacag cttgctatca ccagccctca tcgccgatga
     5641 gcggcacgaa ggccaccggc gcgagcattt tttccgccca ggtgtcggcc gttgggcgag
     5701 taatgaggat caggttctgc tggtggcgat tttggccgac ggggatgacc atgcggccgc
     5761 cggccgccag ttgccggcgc agcgattcgg ggacggtggg gccgccggcg gcaacgatga
     5821 tgccgtcgta gggcgcgtgt tcgggccagc cgcgcgtgcc gtcaccatga tgtacccaca
     5881 cattgttgta acctaatcgg gccagtcgct cgcgggtga
//
LOCUS       DAOMQG010000204         5907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5697_length_5907_cov_9.406357, whole genome shotgun sequence.
ACCESSION   DAOMQG010000204 DAOMQG010000000
VERSION     DAOMQG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_5907_cov_9.406357"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLC06_11075"
     CDS             <1..585
                     /locus_tag="PLC06_11075"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRO91029.1"
                     /translation="PMSVRNAILGLLAQQPRHGYELRAAFEALIGGDDIWNVKPAQIY
                     TTLSRLEEAGLVHRDGVEQDGGPEKRIYSLTTSGRHELAEWFVTGVAAEHTRDEFFVK
                     LMVSLYSEEIDPYQVIRAQRGRLYRDLHALTTRRNGTDARRELAQIFLLDKSIMHLEA
                     DLRWLDLLEARLDDIRHQPLPQPEVKPRGRPRKS"
     gene            652..1395
                     /locus_tag="PLC06_11080"
     CDS             652..1395
                     /locus_tag="PLC06_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91030.1"
                     /translation="MSLISAVDLTKVYGSGDTAVMALDHVSLQVGPGEFVAVMGPSGC
                     GKSTLLHLLGGLDRPTSGSVTIDGQALSNLSDAKLTELRRHQIGFIFQFFNLIPVLNA
                     VENAALPVTLDGVSQAEAKERAKEWLIRFGLGDRLGHRPDQLSGGQQQRVAVARALVA
                     DPALILADEPTGNLDTRASEEIAGLLRQVANDWGRSVVVVTHDPRIAAHADRIIFLKD
                     GRIVDQTVLEPKNGQNAELVADRMLAFGS"
     gene            1449..3998
                     /locus_tag="PLC06_11085"
     CDS             1449..3998
                     /locus_tag="PLC06_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRO91031.1"
                     /translation="MNIQTTLAFRYLGGRKLRTTLTTLAIVFGVLVIFGVNAMLPAFM
                     QAFTANTMAAAGEIDAVITLKTADVFEASTVDQVAAVDGVRAASGLLNRPVNLPADFF
                     DDDPATADAVSAVSLVGIDPEQARTIHAYRIKEGRFLEGDAPETLITESLAEALGLGV
                     GDTLPLPTVVGETSLTIAGILPPRLMPGNEELLVTLPQAQKMLDMPGRINTIEVNFDT
                     LDEAERVAIETRILDALGEQYQVGALEANAELLTNMRVGQSIFNLLGVLALLMGGFII
                     FNTFRTVVAERRRDIGMLRALGANRATIFGIILSEGLIQGVVGTALGLILGYVLAVGS
                     LAAMEPLIHQFMNAQFGSPIVSPGLVMGSVVIGIGMTLLAGLLPAMSASRVTPLEALR
                     PAVGAVSFRGLAGAGFWAGVTLIALAVVALLTRNMALISIGGVLFVVGLILAAPALVN
                     PVAQLFGGLLAAVFARGGTAQLAEGNLSRQPTRAAITAGTTLIGMAILIMAASMVSSV
                     YLGFGEVMRKSLGSDYLLIPPSIGIWGTNVGASGVLADELRAIDGVAVVSSLRFAPTR
                     VGETAVSLLGIDPVTYPEVSGLTFAEGDETAYGALADGRATIINPILATTVGAKIGDE
                     IEFITPTGPQRYRVAAIGGDYLNAKLTTAYISQANIGADFGREEDVLLQLNVAPGADK
                     GAVESAMRAVLKQYPQFRLVDGQAYVEENLEIFNAAFAGIYALVLFLAIPSLIAMLNT
                     LAIGVIERTREIGMLRAVGATRGQVRRIILSEAIILAGLGTVFGVLAGLYLGYMGVEA
                     MRAAGYPMVYVFPTTGVLIAVAAGVLFGALAAIIPARQASRLEIVRALRYE"
     gene            complement(4009..4824)
                     /locus_tag="PLC06_11090"
     CDS             complement(4009..4824)
                     /locus_tag="PLC06_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HRO91032.1"
                     /translation="MMDEKLEELVAAVLSSAKYRHVSPDFVRSIGARELAIRPNLKAA
                     VKATKNALHQAGGAFQDAPIHYERALARLREAWSGDPTDDAFREACRAVMAAHTSTRE
                     RLPILDEFYATVFAALPAPPRRVLDIACGLNPLARPWMPLPPTTEYLACDIYADQMAF
                     LNEFMALCGYAGRAEVRDVIGAPPNEPVDLALVLKTLPCLEAIDKNATARLLDTIRAP
                     WLLVSFPAQTLGGRRKGMAAQYETRFRALLAERGLSAQLFEFKTELAFLIRNS"
     gene            complement(4857..5552)
                     /locus_tag="PLC06_11095"
     CDS             complement(4857..5552)
                     /locus_tag="PLC06_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91033.1"
                     /translation="MKITISGILTDFKGRIFLQQDSPTSLAVLHHRLAVGTTPVDVLD
                     QAFRQETGLIVMPVRMTGVFYDGSIPGGELIFCFRCTMRGGELAVPDGGLPAGFFDCP
                     PLPEGLSSKFRQQVENGLHHPGGPPTLDTVASSVGRRLAGLFGRGKPADDSEPWRVAV
                     RLTDQPVGNGVEFALDNEDEMHPATPVAGGEPPWAAARRLAGAGHQAVELRRVEIGAD
                     RPILILDFAPVGQ"
     gene            complement(5642..>5907)
                     /locus_tag="PLC06_11100"
     CDS             complement(5642..>5907)
                     /locus_tag="PLC06_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91034.1"
                     /translation="QESKHGYPFGVSSFHSVTPLDGFAGGITHRLFRERWNKLSNMTN
                     PTLDGTQFPISSTISDTISQGAGSPCVAPQIAPGPTVVYHNRL"
BASE COUNT         1053 a         1813 c         1939 g         1102 t
ORIGIN      
        1 cctatgtccg tccgtaatgc aatcctgggc ctgttggcac aacaaccccg ccatggctat
       61 gaactgcggg cagcctttga ggcgctcatc ggcggcgacg acatctggaa cgtcaaaccg
      121 gcccagattt acacgacttt gtcgcgcctt gaagaagcag ggcttgtgca tcgggacggg
      181 gttgagcagg acggcgggcc ggagaaacgc atctacagcc tgacgacgag cggccgccac
      241 gagctggccg aatggttcgt caccggcgtg gccgccgagc acacacgcga cgagtttttc
      301 gtcaagctga tggtcagtct gtatagcgag gagatcgatc cctatcaggt cattcgcgcc
      361 cagcgcggcc gtctctatcg cgatctccac gccctgacaa cccgacgcaa cgggacggat
      421 gcccggcggg agctggcgca gatttttctg ctggacaagt cgattatgca tctggaagcc
      481 gacttgcgtt ggctcgattt gcttgaggcg cggctggacg acattcgcca ccaaccgctg
      541 ccgcagccgg aagtcaagcc gcgcgggcgg ccgaggaaat catagggtaa tcgtcaatat
      601 tgttttggaa gagggaagac attcctgtct gccccacgtg aaggagaact catgtctctt
      661 atatctgctg ttgatctcac caaagtctat ggctccggcg acacggccgt gatggcgctt
      721 gatcatgtct cgctgcaggt ggggccgggc gaattcgtgg ccgtcatggg gccgagcggc
      781 tgcggcaagt cgacgctgct gcatctgctc ggcgggctgg atcggccgac gagcggctcg
      841 gtcacgattg acgggcaggc gctatcgaac ctgtcggacg caaagctgac tgagttgcgc
      901 cgccaccaga tcggcttcat cttccagttc ttcaacctta tccctgtcct caacgcggtg
      961 gaaaacgccg ccctgccggt gacgctcgac ggcgtatcgc aggcggaggc caaggagcgg
     1021 gccaaggaat ggctgatccg ctttggcctg ggcgaccggc tgggccatcg ccccgaccag
     1081 ctatcgggcg ggcagcagca gcgggtggcc gtggcccgtg ccctcgtggc cgatccggcg
     1141 ctcattctgg ccgatgagcc gaccggcaat ctggacacgc gcgcctcgga agagatcgcc
     1201 gggctattgc ggcaggtggc caatgattgg ggccggtcgg tggtcgtcgt cacccacgac
     1261 ccgcgcatcg ctgcccacgc cgaccgcatt atcttcctga aggacggccg catcgtcgac
     1321 cagaccgtcc tggagccgaa gaacggccaa aacgcggaac tggtggccga cagaatgctg
     1381 gcgtttggtt cgtaacaata gagacctgac aggttttccg aaaacctgtc aggtctggaa
     1441 gagaaaccat gaatatccaa accacactcg cttttcgcta tctgggcggc cgcaagctgc
     1501 gcacgacatt gaccacgctg gccattgtct tcggcgtgct ggttatcttc ggcgtgaacg
     1561 ccatgctgcc ggctttcatg caggcgttca cggccaacac catggccgcc gccggagaga
     1621 tcgacgccgt gatcaccctt aagacggccg atgtgtttga agcatcgacc gttgaccagg
     1681 ttgccgctgt cgatggcgtg cgtgccgcgt cgggcttgct caatcgcccc gtcaacctgc
     1741 ccgcggattt cttcgacgac gaccccgcca cggccgacgc ggtcagcgcg gtgtcgctgg
     1801 tggggatcga ccccgagcag gccagaacta tccacgccta tcgcatcaaa gagggtcgct
     1861 tcctggaagg cgacgcgccg gagacgctga tcaccgaatc gctggccgag gcgcttgggc
     1921 tgggcgtggg cgacacgctg ccgctgccga cggtggtggg cgaaacatcg ctgactatcg
     1981 caggcatcct gccgccgcga ctgatgcccg gcaacgagga gctgctggtc accctgccgc
     2041 aggcccaaaa gatgctcgac atgcccggcc ggatcaacac catcgaggtc aacttcgaca
     2101 cgctcgacga agcggagcgc gtggcgattg agacacgcat cctggacgcg ctcggcgaac
     2161 aatatcaggt cggcgcgctg gaggctaatg ccgagctgct gaccaacatg cgggtgggtc
     2221 agtcgatatt caatctgctg ggcgtgctgg cgttgttgat gggcgggttc atcatcttca
     2281 acaccttccg cactgtggtg gccgagcggc ggcgcgacat cggcatgttg cgggcgctgg
     2341 gagccaatcg ggccacgatc ttcggcatta tcctgagcga agggttgatc cagggcgttg
     2401 tcggcacggc gctcggcctg attctgggct atgtgctggc ggtgggcagt ctggcggcga
     2461 tggagccgtt gattcatcag ttcatgaacg cccaattcgg ttcgccgatc gtgtcgcccg
     2521 gcctggtgat gggcagtgtc gtcatcggca tcggcatgac attgctggcg gggctgctcc
     2581 cggcgatgag cgccagccgg gtgacgccgc tggaagcgct gcggccggcg gtgggggcgg
     2641 tgtcgtttcg cgggttggcc ggcgccggct tctgggccgg ggtgacgctg atcgcgctgg
     2701 ccgtggtcgc tctgctgacg cgcaatatgg cgctcatcag catcggcggc gtgctgttcg
     2761 tcgtggggct gatcctggct gcgccggcgc tggtgaaccc ggtggcgcaa ctgttcgggg
     2821 ggctgctggc ggccgtcttc gctcgcggcg gcacggccca gctggccgag ggcaacctct
     2881 cgcgccagcc gacccgcgcg gccatcaccg ccggcaccac gctgatcggc atggccatcc
     2941 tgatcatggc cgcgtcgatg gtctccagcg tctatctggg ctttggcgag gtgatgcgca
     3001 agagcctggg cagcgattat ctgctcatcc cgccatctat cggcatttgg ggaacaaacg
     3061 tcggcgcgtc gggcgtgctg gccgatgagt tacgcgcgat cgacggcgtg gccgtggtca
     3121 gttcattgcg ttttgcgccg acgcgcgtcg gcgagacggc cgtttcgctg ctgggcatcg
     3181 atccggtgac ctaccccgaa gtgagcggcc tgacgtttgc cgagggagat gagaccgcct
     3241 atggcgcgct ggccgacggc cgagccacca ttatcaaccc gatcctggca acgaccgtcg
     3301 gggcgaagat cggcgacgag attgagttca tcacccccac agggccacag cgctatcggg
     3361 tggcggccat cggcggcgac tatctgaacg ccaagctcac caccgcctac atttcgcagg
     3421 ccaacatcgg cgcggatttc ggccgcgagg aagatgtgct gttgcagttg aacgtggcgc
     3481 ctggggcgga taaaggggcg gtggagagcg ccatgcgggc ggtattgaag caatatcccc
     3541 agttccgcct ggtcgacggc caggcgtatg ttgaggaaaa cctggagatt ttcaatgccg
     3601 cctttgccgg catctacgcg ctggttctct ttctggccat cccgtcgctc attgccatgc
     3661 tcaacacgct ggccatcggc gtcatcgagc gcacacggga gatcggcatg ttgcgcgcgg
     3721 tgggcgccac gcgcggccag gtgcgccgga tcatcctgag cgaggcgatc atcctggccg
     3781 ggctgggcac agtgttcggc gtgctggccg ggctatatct gggctacatg ggcgtcgagg
     3841 cgatgcgggc ggcgggctat ccgatggtct acgtgttccc gacgacgggg gtgctcatcg
     3901 ccgtggccgc gggtgtcctg ttcggcgcgc tggcggcgat catcccggcg cggcaggcct
     3961 cgcggctgga gatcgtgcgg gcgctgcgat acgagtgaga atcaggaatc aggaattacg
     4021 aatcaggaaa gctaattctg ttttgaattc aaatagttga gccgacaggc cgcgttcggc
     4081 gagcaaggcg cggaaacggg tctcgtattg ggcggccatc cctttgcgac ggccgcccaa
     4141 tgtctgggcg gggaaggaga cgagcaacca cggggcgcga atcgtgtcaa gcagacgggc
     4201 cgtggcgttt ttgtcgatgg cttcaaggca gggcagcgtc ttcagaacaa gcgccagatc
     4261 gaccggttcg ttgggcggcg cgccgatgac gtcgcgcacc tcggcccggc ccgcatagcc
     4321 gcaaagcgcc atgaactcgt tcagaaaggc catctggtcg gcgtagatgt cgcacgcgag
     4381 atactcggtg gtgggcggca ggggcatcca cggtcgggcc agcggattca gtccgcaagc
     4441 gatatcgagg acgcggcggg gcggggcagg caaagcggcg aagaccgtcg cgtaaaactc
     4501 atccaggatg ggcaaccgct cgcgggtgga agtatgggca gccataacgg cgcggcaggc
     4561 ctcgcggaag gcgtcatccg tcggatcgcc actccaggcc tcgcgcagtc gggccagcgc
     4621 ccgttcgtaa tggatgggcg cgtcctggaa tgcgccgccc gcctgatgga gcgcgttctt
     4681 ggtggccttg acggccgcct tcaggttggg ccggatcgcc aactcccgtg ctccgatgga
     4741 gcgaacgaaa tccgggctga cgtggcgata tttcgcgctc gacagcacgg ccgcaaccaa
     4801 ttcttccagc ttctcgtcca tcatatcagt tcggtattct tccaattggc aaccgtttat
     4861 tggccaaccg gggcgaagtc gaggatcagg atcggccgat cggccccaat ctctacccga
     4921 cgcaattcga cggcctgatg accggccccg gccagccgcc ttgccgcggc ccacggcggc
     4981 tctccgccgg cgaccggcgt cgcgggatgc atctcgtcct cgttgtccag cgcgaattcc
     5041 accccgttcc cgaccggctg gtcggtcagt ctgacggcga cgcgccatgg ctcgctatcg
     5101 tcggccggtt tgccgcggcc gaacaacccg gccaaacgac gccccacgct gctcgccacg
     5161 gtatccagtg tgggcggccc gcccggatgg tgcaggccat tctccacttg ctgcctgaac
     5221 ttcgacgaca gcccttccgg cagcggcggg caatcgaaga acccggccgg caggccgccg
     5281 tcgggcacgg ccagttctcc gccccgcatg gtgcagcgaa agcaaaagat caactccccg
     5341 ccgggaatgc taccgtcata aaacacgccc gtcattcgca ccggcataac gatcagcccc
     5401 gtctcttgcc ggaaagcctg atccagcacg tcaaccggcg ttgttccgac cgccagccgg
     5461 tgatggagaa cagcgaggga agtcgggctg tcctgttgca ggaaaatcct gcccttgaag
     5521 tcagtaagaa tgccgctgat tgtgattttc atcgattgcg ctcaatgcgt gccgccagtt
     5581 tttgtgacca tagtactacc gagcttgccc gatatttatg aactactcac gttaacgcct
     5641 gctaaaggcg gttatgatag accactgtag ggccgggtgc tatctgtggc gccacacaag
     5701 gcgagccggc accctggctg attgtatcgg aaatggtact ggaaatcggg aactgcgtac
     5761 catcaagcgt cgggttggtc atattgctca atttattcca cctttcccgg aataatcgat
     5821 gcgtgatacc cccggcaaaa ccatccagag gagtaacaga atgaaacgaa ctgaccccaa
     5881 atggatagcc atgcttgctc tcctggc
//
LOCUS       DAOMQG010000205         5882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5733_length_5882_cov_8.876952, whole genome shotgun sequence.
ACCESSION   DAOMQG010000205 DAOMQG010000000
VERSION     DAOMQG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5733_length_5882_cov_8.876952"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLC06_11105"
     CDS             <1..450
                     /locus_tag="PLC06_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO91035.1"
                     /translation="VFARMTVLENLEMGAFHRSDKAGILEDIEKIYELFPRLKERRTQ
                     LAGTMSGGEQQMLVMGRAMMSRPSLLLLDEPSMGLAPVLVEAIFNTIVDINKQGTTVL
                     LVEQNALMALNIADRGYVLQTGEIVLSDNAKALQNNAMVQKVYLGIE"
     gene            607..1602
                     /locus_tag="PLC06_11110"
     CDS             607..1602
                     /locus_tag="PLC06_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRO91036.1"
                     /translation="MKLQLYSAADVNRALPMAEAIEGVKAGYVQLSAGRVQSPLRTPL
                     NVSPDDVTLIMPFYAPPAAEADSGALGLKLVSVFGSNTGRGLPLIHSIVMAVDASSGA
                     PLALIEGGSLTAIRTGAASGAATDVLARPDASVVAIFGSGVQARRQLEAVCTVRPIER
                     AYVYSLMGAEAFAAEMSGRGPIPDEIIIAATPREALAAADVVCAATTTRTPVFDGNDV
                     RPGTHINGIGSFTPEMQEIDALTVRRASVFVDSVEAVMAEAGDLIIPLNAGEIDLDHI
                     STELGEVIAGHKPGRTSAEQITFFKSVGVSVQDAMAARIILRNGPALGLGATVEL"
     gene            1599..2759
                     /locus_tag="PLC06_11115"
     CDS             1599..2759
                     /locus_tag="PLC06_11115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRO91037.1"
                     /translation="MSDYPSSAQVVVIGGGVMGASTAYHLALRGVRDVVLLESQSFFG
                     QGATGKCAGGIRYQFSTAVNVRLSLLSLPMLARFEEETGQPIDLRYPGYMLLATTEDN
                     VAEFQRNVALQHSLGVPTEWLTGDEVRRRVPQLSAGDVLAATYNPGDGLADPNGVVAG
                     YIGAGRRLGVRAFNDAPATGIEVENGRAVAVTTPLGRIACETVVNAAGPWAALIGDMA
                     GVPLPIKPVRRQMLTTGPLPEVPPDFPFIIDFARSLYFHREGEGLLTGMSNPNQAPGF
                     DESIDDDWELTHLEAAVERLPLLANARLQAHWAGLYEVTPDAHPIIGRVSNLENFVVV
                     AGFSGHGFMHGPIAGLLMSEIILDGRAHTLDIAELGYERFADGRLIREYNVI"
     gene            complement(2752..5649)
                     /gene="recQ"
                     /locus_tag="PLC06_11120"
     CDS             complement(2752..5649)
                     /gene="recQ"
                     /locus_tag="PLC06_11120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRO91038.1"
                     /translation="MLVTIVARTRRGHAACIGGITDDGRSVRLIAPDASSNEYHNIDY
                     RVGEVWDIEGERPDELIAPHVENLVVRRKQRAGWADALPDLIERHMPPHIGGLEALYD
                     GLAQGQPGGPLFITERIGIPAYSTQFWRPDQPLVRDISGKRIRYRYPTDDGGRTITFT
                     GFQDPPEMLPAGVLLRVSLAHWWRPEEHPEAELRCYLQLSGWFLPDDPQTDEEYEDEA
                     PEPEFDNELQPDSSLAEARLMLRRTFGYDDFRPHQAEIIRNVLDRRDTLAVMPTGSGK
                     SMCFQLPALLFPGLTVVVSPLISLMEDQVMQLRQSGVPAAYLNSTLAYDGYVIIMQQI
                     RAGRIKLLYTSPETLLRPETLLMLDDCRVDCLTIDEAHCISEWGHDFRPDYRQLVQVR
                     HRLPDAVCLAVTATATERVRRDIKQTLGVGDADEFVSSFDRENLFLAVNSRGASLAQI
                     ETFLENRRGESGLIYCATRERTEAVTGQLAARGWNALPYHAGLDYAVRRENQHRFAHE
                     EGIIVVATIAFGMGINKSNVRFVIHHDLPKDLESYYQQIGRAGRDGLPADCLLLFSRQ
                     DMMTNQFLIGQGESTQQPAASARLQAMLAFAETSDCRRRPLLAYFGEIDIPEKCGRCD
                     NCLAGERERVDLTVPAQKFLSCVARTEQIFGASHIIDILRGSKSKEVLKRGHDRLSTY
                     DIGREFKKPAWQQLARQFVSDGLLIHDMNHGSLKLTPKGRAVLKGEPYLGTPPEIERE
                     RSPRHSADQPQNYDVELFERLRSLRLKLATEAGLPPYVIFSDATLREMAVYYPQTRDS
                     FIRMQGVGEAKLARYGEMFLAEIRAYCGERGITEREKVTPATARNISRPQIRRTRQDD
                     VVELYQKGWSVTEIAESFGSQNKTVISHLWKSIQDGQDIAPEPLLGESRLSPDERERV
                     LALFAQHGTDFLRPVYDALEESVPWEELHVLRLYFVTKALKEDTSQSDDAR"
     gene            complement(5758..5865)
                     /locus_tag="PLC06_11125"
     CDS             complement(5758..5865)
                     /locus_tag="PLC06_11125"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91039.1"
                     /translation="MALMCVEREPAACHRSLLAERLRQAWGVEVVHLVP"
BASE COUNT         1022 a         1767 c         1948 g         1145 t
ORIGIN      
        1 gtcttcgccc gcatgaccgt gctggaaaac ctggagatgg gcgcgttcca tcgcagcgac
       61 aaggcgggga tccttgagga tatcgagaag atctatgagc tgttcccgcg cctgaaggaa
      121 cgtcgcaccc agttggcggg cacgatgtcg ggtggcgagc agcagatgct ggttatgggc
      181 cgagcgatga tgtcgcggcc gagtttactg cttctcgacg agccttcgat gggccttgcg
      241 cccgtcctgg tagaagcgat cttcaacacc attgtcgata tcaacaaaca aggcacgacc
      301 gttctgctgg ttgagcaaaa cgcgttgatg gcgctcaata ttgccgatcg cggctacgtg
      361 ttgcagacgg gagagatcgt cctcagcgac aatgccaagg cgctgcaaaa caacgccatg
      421 gtccaaaaag tgtatttggg tatcgagtag gggcaggcta caaagttgtc cctcgttata
      481 gaggattaaa gggtgggttt caggtcttct tgcaagaagg ctaaaaccca ccctccgccg
      541 taagtgatga aagggtggga gtcccacttg ctcctgatac ggggacgatc ccaatctgtg
      601 tgatccatga aactccagct ctattccgcg gccgacgtca atcgcgcctt gcctatggcc
      661 gaggctatcg aaggcgtcaa ggccggctat gttcaactct cggcggggcg ggtgcagtcg
      721 cctttgcgca caccgctgaa cgtctcgccc gatgacgtga cgctgatcat gcccttttac
      781 gcgccaccgg cggccgaggc cgatagcggc gcgctggggc tgaaactcgt ctccgtcttc
      841 ggcagcaaca cggggcgcgg tctgccgctc atccactcca tcgtcatggc cgtggatgcg
      901 agtagcggcg cgccgctggc gctcatcgag ggtggcagcc tgacggccat ccgcaccggc
      961 gcggcctcgg gcgcggccac cgacgtgctg gcccggcccg atgcgagcgt ggtcgctatt
     1021 ttcggcagcg gcgtccaggc gcggcggcag ctggaagcgg tctgcacggt gcggccgatc
     1081 gagcgtgcat atgtctatag cctgatggga gccgaggcgt ttgcggccga gatgtccggg
     1141 cgcggcccga taccggacga gattatcatc gccgcaaccc cgcgcgaggc gttggcggcg
     1201 gctgatgtcg tctgcgccgc caccacgacc cgcacgcccg tgttcgacgg caacgatgtg
     1261 cgtcccggca cccatatcaa cggcatcggc tccttcaccc cggagatgca ggaaatcgac
     1321 gcgctgaccg tccggcgggc gagcgtcttt gtcgattcgg tcgaggcggt gatggccgag
     1381 gcgggcgacc tgattatccc gctcaacgcg ggcgagatcg acctcgacca tatcagcacc
     1441 gagttgggcg aggttatcgc cgggcacaag ccgggccgaa ccagtgccga gcagatcacc
     1501 tttttcaagt ccgtcggcgt gtcggttcag gacgcgatgg cggcccggat tatcttgcgc
     1561 aatggccccg cgttggggct gggcgcgacg gttgaactat gagcgattat ccatcttcag
     1621 cccaggtcgt cgtcatcggc ggcggcgtca tgggcgcatc cacagcctat catttggcgt
     1681 tgcggggcgt tcgcgatgtc gtcttgctgg agagtcagag ctttttcggg cagggggcta
     1741 ccggcaaatg cgccggcggc atccgctacc agttcagcac ggccgttaac gtgcgcctgt
     1801 cgttgcttag tttgcccatg ttggcccgct tcgaggaaga gaccggccag cccatcgacc
     1861 tgcgctaccc cggctacatg ctgctggcga cgaccgagga taacgtggcc gaattccagc
     1921 gcaacgtggc cctgcagcac agccttggcg tacccacgga atggctgacc ggcgacgaag
     1981 tgcggcggcg cgtgccgcaa ctgtcggccg gcgacgtgct cgccgctacc tacaatcccg
     2041 gtgacggtct ggccgacccc aacggggtgg tggccggata catcggcgcc gggcggcggc
     2101 tgggcgtgcg cgcgttcaac gacgcgccgg cgacaggcat cgaggttgag aacggccgcg
     2161 ctgtggcggt cacgactcct ctcggccgca tcgcctgcga gacggtggtc aacgccgccg
     2221 ggccgtgggc ggcgctcatc ggcgatatgg ccggcgtgcc cctgcccatc aagccggtgc
     2281 gccggcagat gctgacgacc ggcccgttac cggaagttcc gcctgatttc ccgttcatta
     2341 tcgactttgc ccgcagtctc tacttccacc gcgaggggga ggggttgttg accggcatgt
     2401 cgaaccccaa ccaggccccc ggttttgacg agagtatcga cgacgattgg gagttgaccc
     2461 atctggaggc ggccgtcgag cggctgccgc tgctggccaa cgcccggctg caggcccact
     2521 gggcgggtct ctatgaggtg acgccggacg cccatcccat cattggccgt gtatcgaacc
     2581 tggagaactt cgtcgtcgtc gccggcttct ccggtcacgg cttcatgcac gggccgatcg
     2641 ccgggctatt gatgagcgag atcatcctcg acggccgcgc ccacaccctc gacatcgccg
     2701 aactgggcta cgagcgcttc gccgacgggc gactcatccg tgagtataac gttatctagc
     2761 gtcgtcagat tgggaagtat cctctttcag ggccttcgtc acgaagtaga gacgtaatac
     2821 gtgcagctct tcccagggca cggattcttc aagggcatca tacaccggcc ggagaaagtc
     2881 cgtgccatgt tgagcgaata acgccaggac gcgctcccgt tcgtcggggg agaggcgcga
     2941 ttcgcccagc aagggttccg gggcgatatc ctgaccgtct tgaatcgatt tccacaggtg
     3001 gctaatcacc gtcttgttct gtgagccaaa gctctcggcg atttcggtca cgctccagcc
     3061 tttttgatat aactccacga catcatcttg tcgtgtacgt ctgatttggg gccttgatat
     3121 gttccgggca gtggccgggg taactttctc ccgttccgta attcctcgct cgccgcaata
     3181 cgcccgtatc tccgccagaa acatctcgcc atatcgcgcc agcttggcct cgccgacgcc
     3241 ttgcatgcgg atgaaggagt ctcgcgtttg cgggtagtag acagccattt cgcgtagagt
     3301 ggcgtcggag aagatgacat agggtggcag tccggcttcg gtcgccagct tcaggcgcag
     3361 gctgcgcaac cgctcgaaca gttcgacgtc gtaattctgt ggctggtcgg cggagtgacg
     3421 cggcgatcgt tcccgctcga tctccggcgg cgtgcccaga tacggctctc ccttcagcac
     3481 cgctcgtcct ttcggcgtca gcttcaggct gccgtggttc atgtcgtgta tcagcagccc
     3541 gtcgctaaca aattgacgtg ccagctgctg ccacgccggt ttcttgaact ctctgccaat
     3601 gtcgtaggtg gaaagccggt cgtggccgcg cttcaacacc tctttcgatt ttgagccgcg
     3661 caggatgtcg atgatatgcg atgcgccgaa gatttgttcc gtgcgggcga cgcaggagag
     3721 gaacttctgg gcggggacgg tcaggtcgac gcgctccctc tctccggcga gacaattatc
     3781 gcagcggccg cacttctcgg ggatgtcgat ctcgccgaag taggccagga gcggacggcg
     3841 gcggcaatcg ctcgtctcgg cgaaggcgag catggcttgc agccgggcag atgccgcggg
     3901 ttgttgtgtc gattccccct ggccgatcag gaactggttc gtcatcatat cctgccggct
     3961 gaagagcagc agacagtcgg cgggcaggcc atcgcgtccg gcacggccga tttgctggta
     4021 atagctctcc agatccttgg gcaggtcatg gtggatgacg aagcgaacgt tggatttgtt
     4081 gatacccatg ccgaaggcga tggtggcaac gacgatgatc ccctcttcgt gggcgaaacg
     4141 gtgctggttc tcgcgccgaa cggcgtagtc cagtccggcg tgatagggca gcgcgttcca
     4201 gccgcgcgcg gccaattgcc cggtcaccgc ttcggtacgc tcgcgggtgg cgcagtagat
     4261 gaggcccgac tcgccccggc ggttttcgag gaacgtctcg atctgggcca ggcttgctcc
     4321 gcgtgagttg acggccagaa acagattctc acggtcgaac gaggagacaa actcgtcggc
     4381 atcacccacg cccagcgttt gcttgatgtc gcgccggacg cgctcggtgg cggtggccgt
     4441 cacggccagg cagacggcgt cgggcaggcg atggcgcacc tgcaccagtt ggcgatagtc
     4501 cggccgaaag tcgtggcccc attcggagat gcagtgagcc tcgtcgatgg tcaggcaatc
     4561 cacacggcag tcgtcgagca tgagcagcgt ttccggccgc aacaacgtct ccggcgaggt
     4621 atagagcagt ttgattcgcc cggcgcgaat ctgttgcatg atgatgacgt agccgtcata
     4681 ggccagagtg ctgttgagat aggcggccgg cacgcccgat tggcgcagtt gcatcacctg
     4741 gtcttccatg agggagatga gcggcgagac gacgaccgtc aggccgggga agagcaaggc
     4801 gggcaattgg aagcacattg acttgccgct gccggtgggc atgacagcca gcgtgtcgcg
     4861 tcgatcgagg acgttgcgga tgatctcggc ctgatgaggg cggaagtcat cgtagccaaa
     4921 ggtgcgccgg agcatgaggc gggcttcggc caaagaggag tcgggttgta gctcgttgtc
     4981 aaactccggt tccggtgctt cgtcttcgta ctcttcgtcc gtttgcggat cgtcgggcag
     5041 aaaccagccg gagagttgca ggtagcagcg cagttccgct tcgggatgct cttccggccg
     5101 ccaccagtgg gcgagagaga cgcgcaggag taccccggcc ggcagcattt ccggcgggtc
     5161 ctggaagccg gtgaaggtga tcgttcggcc gccgtcgtcg gtggggtagc ggtagcggat
     5221 gcgcttgccg ctgatgtcgc ggacaagtgg ctggtcaggt cgccagaact gggtgctgta
     5281 ggcggggatg ccgatgcgct cggtgatgaa cagcgggccg cccggttgtc cctgggccag
     5341 accgtcgtag agcgcttcca acccaccgat atggggcggc atgtggcgct cgatgagatc
     5401 ggggagcgcg tcggcccagc ccgcgcgttg cttgcgacgg acgacgaggt tctcgacgtg
     5461 aggagcgatg agttcgtcgg gccgttcacc ctcaatatcc cagacctcgc cgacgcggta
     5521 atcaatatta tgatattcgt tggatgaggc gtcgggggcg ataaggcgta cggagcggcc
     5581 gtcgtcggtg atgccgccaa tgcaggccgc gtggccccgg cgtgttctgg cgacgattgt
     5641 tacgagcatg aatatcctcc cgggaacggc gaactgtggt tgacgacgga cgaccggcca
     5701 tcgaccgcca gcacccttca ttcgtcactc gtcgctcgtc attcgtcact tcttccgtta
     5761 tggtactaaa tgaaccacct cgacgcccca cgcctgacgc aggcgctcgg ccaggagcga
     5821 gcggtgacag gcggcgggct cgcgctcgac gcacatcagg gcgatgacgg ccgcgtcggg
     5881 tg
//
LOCUS       DAOMQG010000206         5864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5757_length_5864_cov_13.114650, whole genome shotgun sequence.
ACCESSION   DAOMQG010000206 DAOMQG010000000
VERSION     DAOMQG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_5864_cov_13.114650"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..676
                     /locus_tag="PLC06_11130"
     CDS             107..676
                     /locus_tag="PLC06_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91040.1"
                     /translation="MITGLKTLWGWVAILALIIVAACTPAVETEPEAAAYPARAMATT
                     TLPPYPWPMQPPPPTDEPPPTDEPLPTEPPVPTLPPTPVVTPIPTAAPPIIPFPDGTT
                     AQPFTLYWRDGDVIRSLRSEGQEEPSVFLDPVKEFGLYLPPEDIYMILVAETANQGSR
                     PITSGPINQTYLWTAETIIGGNTFLSQGR"
     gene            874..1665
                     /locus_tag="PLC06_11135"
     CDS             874..1665
                     /locus_tag="PLC06_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91041.1"
                     /translation="MSAEIGTLRSVDPDLILVVDDDIFFRESIASFLKRRHFATLEAG
                     DGESALELAVRYKPLAALIDVVLPEHPHDKARKEHSFGVELARQLKRTDPMMAVVLLS
                     AYGDRSDAVLKLAAEGVRGLAYLIKGYRTGTAALLRALEETRAGHVMIQANEPNQTAS
                     LAERFWARLTPEERDFVRRGVELFPTLSPREREIAYAFAHAQTIEGVATDLNISVPTV
                     EKHVNHIYSKLELDRMALRQSPLRKSLLLAKACWLLDLLGGEEKG"
     gene            1665..4124
                     /locus_tag="PLC06_11140"
     CDS             1665..4124
                     /locus_tag="PLC06_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91042.1"
                     /translation="MPTWLRNPTGALLLVVLLVSMAGIASLADKIGHPFGGYASTLFF
                     LPLSAPGGIVVQETPAWWPIRGINPPLDEYTYLLEINDQPYQSADVGAVFERAWDVGA
                     KSVTILVDRSRGGHLATIEVPVIRFDFIHFLELKLPDLITGFCLWLTALAVLKARPQE
                     PLNQVFAVIASLVALQRWTVVNAIWVDLRVIPVIFEFPIMAAAGFIGVVLLHFALLFP
                     RRIEPWPRRLLLVLYGMGVVITVLHMLSRVPAIYRLPEPRKTTLLLWSDRAFLALVVL
                     YLTGITVLFCRMIWFGLRGRRSRRDRRVALIVLIGLMAALPMILITADSVVPGLGVET
                     VPFWRGLDLRYLFVTVPLGFALAIIRYQSMRSPSRLLIVVIALAGSALLAAILSWLWG
                     LSQGNWPANGEHPPFPYLFAGIFAASVFWSTQATWRGWFGRLLEWDTHSYTAARAFGR
                     RVSGTTNLIDLSQTIAAALVNELQLDRAAVWLWKPEPGAFELAAEAGQTDPSLPARLP
                     FSEDYPSEANRPLRLDAADGNPSWLESLNVLRTIDVVVPLQTDGQPIGLLGLGHRWDE
                     EIFDDRDLAIAELVGQQAALFLLAAMQIEELRHVPQQIVAAQESERRRLAAELHDTVQ
                     QFLGGLPFALAFTTDEIRADPEGVAAVLEHSLEDVESMAETVRRIRFNLAPSQLEYSL
                     TRSLSELVAHVAKRAKLRVSLSTGEGLDQATALITREALYRVIQQALDNIVAHAEASE
                     ANLRLECADGRVTFSVTDDGRGSTEAERREADRLGHFGLSAMRQRVELCGGEFTFAST
                     PGEGTTVSGWVPAIGEAATWK"
     gene            4487..>5864
                     /locus_tag="PLC06_11145"
     CDS             4487..>5864
                     /locus_tag="PLC06_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91043.1"
                     /translation="MNRKALFIVAASLTLLVLAAWVGAQEPVPPLPLDAQVSTGSSFT
                     YQGQLKNGGDVFDGSCDFQFRLYDAVSGGSQVGAIQTSTGIGVQEGFFSTNLDFGGEA
                     FDGSARWLGISVRCPTGSGEYITLNPRQALSATPYALSLKPGALVINNASNPDGDPMP
                     TAITGRATASSGHTIAILGEAYSPDGSAVSGDNYSSNGGTGVWGWSGATTGPAAGVRG
                     SANSPQGAGVWGYNSATAGEGKGVLGDSNSPDGSGVFGYNDDGGDGVLGIVDGPKANE
                     GTGVVGLNLATSGEGKGVLGGSNSPDGAGVFGYNDDGGDGVVGISDHGGSGVHGASFA
                     SGDTYGVSGVNYATTGWGIGVYGLSHSDGPAVLAENQANGMGLWTRSAHGNPIEALGN
                     NLSDVEFYVTNTGDVFADGAFQSPAADFAELLPAIPGLETGDVLVIGPDSKLTRSTEP
                     YQTTVVGVY"
BASE COUNT         1127 a         1592 c         1811 g         1334 t
ORIGIN      
        1 aggggtctcg tcccgatcaa cgaccggatt ctggagcgag ccgatcactc ctaatctcaa
       61 acatatagaa cgttgccggg ttttctccga acaggaaggt gtcattatga tcaccggact
      121 caagaccttg tggggatggg tagcaattct ggccctgatc attgtggccg cctgcacgcc
      181 ggccgtcgag acggaaccgg aagcggccgc ttatccggcc cgcgccatgg ccacaaccac
      241 gctgcctccc tatccctggc cgatgcaacc cccgccgccg accgacgaac cgccgcccac
      301 cgacgagccg ctgccgaccg agccacccgt gccgacgctc ccgccgacgc cggtggtgac
      361 gcccatcccg acggccgcgc cgccgatcat ccccttcccc gacggaacga cagcccagcc
      421 cttcaccctc tactggcgcg acggcgacgt gattcgcagc ctgcgcagcg agggccagga
      481 agagccgtcg gtctttctcg acccggtgaa ggagtttggg ttgtatttgc cgccggaaga
      541 tatctatatg atcctggtgg ctgaaacggc aaatcaagga tcgcgaccca taacgagtgg
      601 tccaataaac caaacttatt tgtggactgc ggagacaatt attgggggga acaccttcct
      661 ttcacagggg cgttgatctg tctggggctg gtaccggcca tcgggctggc ggcctgctgg
      721 ttgccgacgg aggggcggcg aggctgacgg gtagggaggc gttggcgtat gagtagggag
      781 tatcataatt tgtgcattgg gtaattccgc cctattgcga tatactcaat aaaccaatat
      841 catcagccca ctatagttcg tttagggggc gtaatgtcgg ccgaaattgg aacgcttcgg
      901 tcagtcgatc ctgatctcat cctcgttgtc gatgatgaca tcttctttcg cgagagtatc
      961 gcttcgtttt tgaagcgacg gcattttgcg acgttggagg caggggatgg cgagtcggcg
     1021 cttgagcttg ccgttcggta taaacctctg gccgctctga tcgacgtcgt cctacccgaa
     1081 catcctcacg acaaggcccg caaagaacat agcttcggcg tcgaattggc ccgccaactg
     1141 aagcggaccg acccaatgat ggctgttgtc ctattgtcgg cctacggcga ccgcagtgac
     1201 gcagttttaa agctggccgc cgagggagtg cgtgggcttg cctatttgat aaagggctat
     1261 cggacgggca cggccgcctt gttgcgggca ctcgaggaaa cccgcgccgg ccacgtcatg
     1321 atccaggcaa acgagccaaa ccagacggct tcgctggctg aacgattctg ggcgcggctg
     1381 acccccgaag agagagactt tgttcggcgg ggcgtagaac tcttcccaac gctttcccca
     1441 cgcgagcgtg agatagcgta tgccttcgcc cacgcccaaa caatcgaagg ggttgccact
     1501 gatctaaata tctcagtgcc cacggtcgag aagcacgtca accacatcta tagcaaactg
     1561 gaactggatc gaatggcact ccgccaatcg ccattgcgca agtcgttact gctggcaaaa
     1621 gcatgctggt tgctcgatct gttgggcggt gaggaaaaag gataatgccc acctggttac
     1681 gtaatccgac gggcgcgcta ttgctggttg ttttgctcgt gagtatggcc gggatagcat
     1741 ccctggccga caaaatcgga cacccatttg gaggttatgc cagcaccttg tttttcctgc
     1801 cactctcggc cccaggcggg atcgtcgttc aagaaactcc ggcttggtgg ccgattcgcg
     1861 gcataaaccc acccttggac gaatatacct atctcctgga gatcaatgat caaccctatc
     1921 aatcagctga tgtaggcgcg gtctttgaga gggcatggga tgtgggcgca aagtccgtca
     1981 ctatcctggt ggaccggtca cgtggggggc acttggcgac gattgaagtg cctgtcatcc
     2041 gcttcgattt tatccacttt ctggaactga aattgccgga tttgatcacc ggtttctgtt
     2101 tgtggttgac cgcgcttgcg gtgcttaagg cacggccgca agagccgtta aatcaggtct
     2161 tcgccgtcat tgcgtcgctg gttgcgctcc aacgctggac agtggttaat gccatctggg
     2221 ttgatctgcg agtgataccc gtgatcttcg agttcccgat catggccgcg gccggattca
     2281 ttggcgtcgt cctgctgcac ttcgcgctgt tgttcccgag gcgaatagag ccgtggccgc
     2341 gacggttgct gctggtcctg tacgggatgg gggtcgtcat cactgttctg cacatgcttt
     2401 cacgtgtgcc tgctatatac cggttgccgg agccgcgaaa gacgacgctg ttgctttgga
     2461 gcgaccgagc ctttctggcc ctggttgtcc tctacctgac aggtatcacg gttttgtttt
     2521 gccgaatgat ctggtttggg ttgcgagggc ggcgctcgcg ccgcgatcgg cgagttgccc
     2581 ttatcgtcct gatcggacta atggcggctc tgccgatgat actgatcacg gccgacagtg
     2641 tggtgccggg cttgggggtg gaaactgtgc ccttttggcg tggcctggac ttgcgctatt
     2701 tatttgtaac cgttcccctg ggttttgccc tggccatcat ccgttaccag agtatgcgca
     2761 gcccctcacg gttgctgatt gtggtcatcg ccctggccgg aagcgcgcta ctggcggcga
     2821 tcctgtcatg gctgtggggc ctgtcccagg gcaactggcc ggccaatggg gagcatcccc
     2881 catttcccta tctcttcgcc ggcatcttcg ccgccagtgt cttctggagc acgcaggcca
     2941 cttggcgcgg gtggttcggc cgcctgctgg aatgggatac ccatagttac acggccgccc
     3001 gggccttcgg ccgtcgcgta tcgggcacga ccaacctcat cgacctgtcg cagacgatag
     3061 ccgcggcgct ggtgaacgaa ttgcagcttg atcgggcggc ggtttggctt tggaagccgg
     3121 aaccgggcgc gttcgagctg gccgccgaag ccggccaaac cgatccatcg ctgccggctc
     3181 gcctgccctt ctctgaagac tacccgtcgg aagcgaaccg gccgttgcgg ctggatgcgg
     3241 ccgatggcaa tccatcctgg cttgagtcgc taaacgttct gcggacgatt gatgtcgtcg
     3301 tcccgcttca gaccgatggg caacctattg gtctgctggg cctggggcac cgttgggatg
     3361 aggaaatctt cgatgaccgc gatctggcga tcgccgaact tgtgggtcaa caggcggcgc
     3421 tattcttgtt ggcggccatg cagattgaag agttacgcca cgttcctcaa caaatcgtgg
     3481 ccgcgcagga gagcgaacgt cgtcgtctcg ccgcggaatt gcacgacaca gtgcagcaat
     3541 tcctgggcgg gttgcctttt gccctggctt ttacgacaga tgagatacgg gccgatcctg
     3601 aaggggtagc cgccgtcctg gaacacagtc tggaagatgt ggaatctatg gccgagactg
     3661 tccggcgcat ccgtttcaac ctcgcgccaa gccaattgga gtacagtcta acgcgttcct
     3721 tgagcgagct tgttgcccac gttgcgaagc gcgccaagct ccgggtatcg ttgagcaccg
     3781 gagagggctt ggaccaggcg acggcattga taacgcgcga ggcgctttac cgggttatcc
     3841 agcaggcact cgacaacatt gtcgcccatg ccgaggcgag cgaggctaat ctccgactgg
     3901 agtgcgccga cggccgggtg acgttctccg ttaccgatga cggccgcggt tcgaccgaag
     3961 cggaacgtcg agaggcggac aggctcggtc actttgggct atccgccatg cggcagcgtg
     4021 tggaattgtg cggtggggag tttacctttg cctctactcc cggtgagggc acgactgtgt
     4081 caggctgggt tccggcgatt ggggaagccg cgacctggaa ataatggttt tcctccattt
     4141 cttatggagg atagccgggt tcaattcacc cctcttctac ctataattta gatatcgcgc
     4201 ggccggtccg ggaatgagta gttgacctgt ggccgatgtt gagcatcggc cacaggtgag
     4261 ggcttctgct tccggttggc gtgttgccct ccgttatgcg cggaacgcgt ggaggggatt
     4321 gtatactgga tgagtgacta cttgcattaa gatgtgaggg tcgggggtgt tttcggtagc
     4381 gttttgcctt aaccttaaga tgttgcctga cagcatggct ggagaattta cctatagtag
     4441 ggctgatttc tagagaagga tttaaggatt taaggaggga aagcacgtga atcgcaaagc
     4501 actctttatt gtagccgctt cgctgacgct cttggttttg gctgcctggg ttggcgctca
     4561 ggagccggta cctcctctac cattggatgc tcaagtatcg accgggtcat ccttcaccta
     4621 tcaaggacaa ttgaagaatg gcggagacgt gttcgacggc tcatgcgatt ttcagttccg
     4681 gctgtatgat gcagtatccg gcggaagcca ggttggcgcg atacagacct ccaccgggat
     4741 aggcgtacag gaaggcttct tttcgaccaa cctcgacttt ggcggcgagg ccttcgatgg
     4801 gtcggcgcgc tggctgggca tcagcgtgcg ctgcccaacg ggaagcggag aatacatcac
     4861 ccttaacccg cgccaggcgt tgagcgcgac gccttatgca ttgagtctga agccaggtgc
     4921 gcttgttatc aataatgcgt ccaatccaga tggcgatcct atgccaacag ctataactgg
     4981 tcgtgctaca gcctctagcg gtcataccat tgcaatactg ggcgaagctt attcgccaga
     5041 tgggagtgct gtttccggcg acaactatag ctctaatggt ggtacgggag tttggggctg
     5101 gagtggtgcg acgacaggac ctgcagctgg ggtacgtggg tcagcgaatt ccccccaagg
     5161 agcaggggtc tggggatata acagtgcaac cgctggtgaa ggcaaaggcg ttttgggtga
     5221 cagtaattcg cccgatggat ctggtgtctt tgggtataac gatgatgggg gtgatggagt
     5281 attagggatt gttgatgggc cgaaagctaa cgaaggtacg ggtgttgtcg ggttaaacct
     5341 ggcaacttct ggagagggaa agggagtttt aggaggcagt aattcgcccg atggcgcggg
     5401 tgtctttggg tataacgatg atggcggtga cggcgtggtc ggtatctctg accacggcgg
     5461 ttcaggcgta catggcgcat ccttcgctag cggagatacc tacggggtat caggggtaaa
     5521 ttacgctacc acaggttggg ggataggtgt atatggactg tctcactctg acgggccagc
     5581 ggtccttgcg gagaatcagg caaatgggat gggcttatgg acacgaagcg ctcatggcaa
     5641 cccaatcgaa gcccttggca ataatctgtc cgacgtagag ttctacgtta ctaataccgg
     5701 tgacgtgttt gccgacggtg ccttccaatc gcccgccgcc gatttcgccg aactactgcc
     5761 ggctataccc ggtctggaaa ccggtgatgt gttggtcatc ggccccgaca gtaagctgac
     5821 gcgcagcaca gaaccgtatc agacgacggt cgtgggcgtc tatt
//
LOCUS       DAOMQG010000207         5860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5766_length_5860_cov_5.910078, whole genome shotgun sequence.
ACCESSION   DAOMQG010000207 DAOMQG010000000
VERSION     DAOMQG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5766_length_5860_cov_5.910078"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1652
                     /locus_tag="PLC06_11150"
     CDS             360..1652
                     /locus_tag="PLC06_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91044.1"
                     /translation="MLFLLVALPVMTAALAQGEGPVAKQLPVVTEPGESAAPETYNVY
                     ESEANNTFATADWLTVNDVMGGMIGSTGDVDYFKFNMPEYWHGMILLDIEAASINSAL
                     DPVVCLYDNNGAIVACNDDSDGLDSVVFAAMWGSGGAGGNYPYYISVRDLGDNSGGSN
                     HFYELIVSNPLLVSAPAANLGTGNVAGIPFQSQDILAFSSLNTGDEKWIMFFDGSDVG
                     LTKNVTNVAAGHWKYPEIFISLMANQNVPGVGTVKPQDVIQFNGQFGPTTSGTFTMYL
                     RGSEWDLTKGSEKIDAFGTWTYGVGGGYEGTCWGFPISMTGAATVSRDGATFKFADED
                     VACMEEFYGLQGWRSYFDGSMAAGLAAEDVNGVSMVDRNNQLYLNILGKGNIAGHAVT
                     QKDIFAVNYPAYTWGGFVWRGPEHGWNYDIDAFEYKGW"
     gene            2020..2379
                     /locus_tag="PLC06_11155"
     CDS             2020..2379
                     /locus_tag="PLC06_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRO91045.1"
                     /translation="MAKDKFTNGYALIIGVSDNLIPTYALPTVAHDAAALHKVLIHPR
                     RCAYDPDNVRLLTGRDATGAGIRAGLSWLKEKIAADRSDNATAVLYLSSPKIPDSPYR
                     FGIIGTKITYSGSGFRL"
     gene            complement(2319..4964)
                     /locus_tag="PLC06_11160"
     CDS             complement(2319..4964)
                     /locus_tag="PLC06_11160"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRO91046.1"
                     /translation="MHAYRSINHRTLLILISTALLVAVAIAWSPFARAADFTAATEAE
                     LVTAIDAANAAGAGSHTITLSADITLTAPLPAIDNSAATAITLDGTGHTLDAGGTGTA
                     LAIMPDTTAAIKNLTLTGGAGSHGSDGHSGGGLFNMGTLTVTDATLSGNTASQGAGIM
                     NAGGEGGSSASLTLTRVTLSDNAATNAGGALANHGNDNTAAATIIDSTIIDNSAGQYG
                     GAIANSGLVGAANLTITRSTLAGNSANLGGGLFNNGNSGQATAALTGVTLSGNEATNT
                     GGGVFNNGNNGSATVTLTNSTLSGNTAGSSGGGLTNTANSGAALVSMRFVTLADNAAK
                     TGGGFYNSAAAVIEASASIFAAGSQGAACAFNGGTALTSGGYNLDSDGSCGLGGAGDI
                     SDGNAGLAGLALNAPGTTATHALDAAGDAQRKVPSGTAGCGTDITTDQRGAARPNPAP
                     ACDIGAYESDAAAGGATAKPTAPVPTATGTPPSPTPTTTGTPPSPTPTSPVPTATGAP
                     PSPTPTATVPAGCAPPYMPGNEAQLNGAIACVNAAGGGTHTITLAADIELSGPTIPLG
                     NPVAAEVILDGAGHTIDGNRKGTVLTISGPTRARIRNITITGGQGSSGPNSNWAGGVF
                     NTGRLTIENSSLFANIAGRGGGVFNYGDGAGASLILIRSTLSSNVATGMGGGIANAAA
                     DGGDAAVRVENTTLAGNYAVAGGGGLFNDAGGGTAGAALIYATLGINTATSGGGGIHT
                     LGSSNQSVVALTATIISNGLGAGPDCVAPVGSILSNGYNLDGDGTCGLNQTGDLSHAN
                     AGLLPLKVNPPGTTATLALGPGSAALNRIANGLAFCGAAIKLDQRGAARPYPAGGACD
                     VGAYEHQSLNPLPEYVIFVPMIPKR"
     gene            5154..5435
                     /locus_tag="PLC06_11165"
     CDS             5154..5435
                     /locus_tag="PLC06_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRO91047.1"
                     /translation="MNDYARQDATQRLASAAGHIRGIERMVNDDAYCIDVIKQIQAVQ
                     AALSKVSTLLLDNHLRTCVTTAIQGDDAVERERMLQEITSVFTVTGKSK"
     gene            5545..5766
                     /locus_tag="PLC06_11170"
     CDS             5545..5766
                     /locus_tag="PLC06_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRO91048.1"
                     /translation="METKTFKVPGISCGHCVHTIKMEVGDVPGVTAVEADPQTKMVTV
                     TWNTPADWEKIRATLVEINYPPEGLISIN"
BASE COUNT         1019 a         1797 c         1953 g         1091 t
ORIGIN      
        1 gttcatgtgt gacgggtgtc tgaacaggat cggattgcag ccgggagtgt gatggggtgg
       61 atgaggccgc ggcgacgctg ctgcgacctc gcaaccgcct tttcatgatc ttcttgtgcc
      121 gtcttatttg tgacatttgt cacaaataga catgacaaac gtcacctgac acggttaatt
      181 taccctacta ccattgttga taggaggaac ctacctatcg cccgtcagcc aggcggcgcg
      241 taccccatcc gtcttgcggc ggacgatccg tatctttccc ccgaactacc actcttttcg
      301 cacgtttgga ttaggagcca aacatcatga ataccaagaa gatggttaag tggggagtcg
      361 tgctgttcct gctggtcgcg ctgccggtga tgaccgcggc gttggcgcaa ggggagggac
      421 cggtggcgaa acaactgccg gtggtaacgg agccgggcga gtcggccgcg cccgagactt
      481 ataacgttta cgagagcgaa gcaaacaaca cgtttgcgac ggctgactgg ctgacagtca
      541 atgacgtcat gggcggcatg atcggcagca caggcgatgt cgattacttc aagttcaata
      601 tgcctgagta ctggcacggg atgatactgc tcgatatcga agcggcctcc attaactccg
      661 cgctggaccc ggttgtttgt ttgtatgaca acaacggcgc tatcgtggcg tgcaacgacg
      721 actcggacgg attggattcg gtcgtcttcg cggccatgtg ggggtccggc ggcgcgggcg
      781 gaaactaccc ctattacatt tccgtgcgcg atcttggcga taatagtggg ggtagcaacc
      841 acttctacga gttgattgtc tcgaacccgc tgctcgtcag cgccccggcg gcaaacctgg
      901 ggaccggcaa cgtggccggg atccccttcc agtcgcagga catcctggcc ttctccagcc
      961 tgaataccgg tgatgagaag tggatcatgt tcttcgatgg ctcggatgtc ggcctgacca
     1021 agaacgtgac caatgtcgcg gccggacact ggaagtatcc ggagatcttc atcagtctga
     1081 tggccaacca gaatgtaccc ggcgttggga cagtcaagcc gcaggatgtg attcagttca
     1141 acggccagtt cggccctacc acgagtggta catttaccat gtatctgcgc ggatcagaat
     1201 gggatctgac caagggtagc gagaagatcg atgccttcgg cacctggacc tacggtgtgg
     1261 gcggtgggta tgaaggtacc tgctggggct ttccgatcag catgaccggc gcggccacgg
     1321 tgtcccgcga cggcgcgacg ttcaagttcg ccgatgagga tgtggcatgc atggaggagt
     1381 tctacggcct tcagggatgg cgcagctact tcgacggctc catggcggcc ggtcttgcgg
     1441 ctgaggacgt aaacggagtg tcgatggttg atcgtaacaa tcagctgtat ctcaatattc
     1501 tcggcaaggg caatattgct ggtcacgccg ttacccagaa ggacatcttc gccgtcaact
     1561 atccggccta tacctggggt ggtttcgtct ggcgcggccc cgagcacggc tggaactacg
     1621 acatcgacgc gtttgagtac aagggctggt agaacgcagg ataatttcag gccgcgagca
     1681 aatcgcggcc tgattcccga ttaggcccaa cgcctcccgg tggaatcccg ccggggggcg
     1741 ttttttcgtc gcaattatcc cggctgcctt cgcggcgctt ttgttacgga acataccacc
     1801 caggatcagc ccccgcccgg ctatcgcgtc gcccgctatc cggtcaatat ttttacatca
     1861 ggcaaactgg cccttgcaat gaattgttgc ataatcccgc atcatcatgc gcccggcggg
     1921 ttatgtgagc ggaaagtcga ccggcgggcg gaccgagcgc gctcgatccg ccccatccgt
     1981 gttcgacgat tccctggccg ggcggccgcg aggtgcggaa tggccaaaga caagttcacc
     2041 aacggctacg cgctgatcat cggcgtgagc gacaacctga ttccgaccta tgccctgcct
     2101 accgtggccc acgacgcggc cgcgctccac aaggtgctca tccatccgcg gcgctgcgcc
     2161 tatgaccccg acaacgtccg cctgctgacg ggccgggacg ccaccggcgc cggcatccgc
     2221 gccggcctgt cgtggctcaa ggaaaagatc gccgccgacc gcagcgacaa cgccaccgcc
     2281 gtcctctatt tgtcaagtcc taaaatacct gattcaccct accgtttcgg aatcatcggg
     2341 acgaagatca catactccgg cagcggattc aggctctgat gctcatacgc gcccacgtcg
     2401 cacgccccgc ccgccggata tggccgcgcc gccccgcgct gatcaagctt gatcgccgcg
     2461 ccgcagaaag ccaacccgtt ggcgatgcga ttgagcgccg cgctgcccgg ccccagggcc
     2521 agcgtcgccg tggttcccgg ggggttgacc ttcagcggca gcagcccggc gttggcgtgc
     2581 gacaaatccc cggtctgatt tagcccgcac gtgccgtcgc cgtcgaggtt gtagccgttg
     2641 gagaggatgc tgccgaccgg cgcgacgcaa tccggcccgg cccccagccc gttgctgatg
     2701 atcgtcgccg tcagagccac caccgactgg ttgctggagc cgagcgtgtg aatgccgccg
     2761 ccgccgctgg tggccgtgtt gatgcccagc gtggcgtaga tgagggccgc cccggcggtg
     2821 ccgccgcccg catcgttgaa caggccgccg ccgccggcca cggcatagtt gccggccaat
     2881 gtcgtgttct ccacccgcac cgccgcgtcg ccgccgtcgg ccgccgcgtt ggcgatgccg
     2941 ccgcccatgc cggtggccac attgctcgac agggtggagc ggataaggat caggctcgcg
     3001 cccgcgccgt cgccgtagtt gaacacgccg ccgccccgcc cggcaatgtt ggcgaagagg
     3061 ctgctgtttt cgatagtgag ccgccccgta ttgaacaccc cgccggccca gttgctgttg
     3121 gggccgctgc tgccctggcc gccggtgatg gtgatgttgc ggatgcgcgc ccgcgtcggg
     3181 ccgctaatcg tcagcacggt tcccttgcgg ttgccgtcga tcgtgtggcc cgcgccgtcg
     3241 aggatgactt cggccgccac cgggttgccc agcggtatgg tcggcccgct cagctcgata
     3301 tcggccgcca gcgtgatggt gtgggtgccg ccgcctgcgg cgttgacgca ggcgatggcc
     3361 ccgttgagct gggcctcgtt gcccggcata tagggcgggg cgcagcccgc gggcacggtg
     3421 gccgttgggg tgggcgatgg gggcgcgccc gtcgcggtgg gcacggggga cgtgggcgtg
     3481 ggcgacgggg gcgtgccggt cgtcgttggg gtgggcgatg gcggtgtgcc cgttgccgtg
     3541 ggcacggggg ccgtgggctt cgccgtcgcg ccaccggcgg ccgcatcgct ctcatacgcg
     3601 ccgatgtcac aggccggggc cggattcggc cgcgccgcgc cgcgctggtc ggtcgtgatg
     3661 tccgtgccgc agccggccgt gcccgatggc accttgcgct gcgcgtcgcc ggctgcatcc
     3721 agtgcgtggg tggccgtcgt gcccggcgcg ttcagggcca acccggccag cccggcgttg
     3781 ccgtcgctga tgtcgcccgc cccgcccagg ccgcaggagc cgtcgctgtc caggttatag
     3841 ccgccggagg tgagggccgt gccgccgttg aaggcgcagg ccgcgccctg gctgcccgcg
     3901 gcgaagatag aggccgacgc ctcgatgacc gccgccgccg agttatagaa accgccgccc
     3961 gttttggccg cgttgtcggc cagcgtcaca aaccgcatcg acaccagcgc cgcgccgctg
     4021 ttggccgtgt tggtcagacc gccgccactg ctgcccgccg tgttgcccga cagggtgctg
     4081 ttggtcagcg tgacggtggc gctgccgttg ttgccgttgt tgaagacgcc gccgccggta
     4141 ttggtggctt cgttgcccga cagggtgacg ccggtcagcg cggccgtcgc ctggccgctg
     4201 ttgccgttgt tgaagaggcc gccgcccaga ttggcggagt tgcccgcgag ggtgctgcga
     4261 gtgatggtca gattggccgc gccgacgagg ccgctgttgg cgatggcccc gccatattgc
     4321 ccggccgagt tgtcgatgat cgtcgagtcg atgatcgtgg ccgcggcggt gttgtcgttg
     4381 ccgtggttgg ccagcgcgcc gccggcgttg gtcgccgcat tatcgctcag ggtcacccgc
     4441 gtcagggtca gcgaagcgct gctgccgccc tcgccgccgg cgttcatgat acccgccccc
     4501 tgcgaggcgg tgttgccgct gagggtggcg tcggtgaccg tcagcgtgcc catgttgaag
     4561 aggccgccgc cggaatggcc gtcgctgccg tggctgcccg cgccgccggt gagggtcagg
     4621 tttttgatgg ccgccgtcgt gtcgggcatg atcgccaaag ccgtgccggt gccgccggcg
     4681 tccagcgtgt ggcccgtgcc gtcgagggtg atggcggtgg cggccgaatt gtcgatggcc
     4741 ggcaatggag cagtcaaagt aatgtcggcc gacagcgtga tggtgtggct gcccgcgccg
     4801 gcggcgttgg cggcgtcgat ggccgtcacc agctcggcct cagtggcggc ggtaaaatcg
     4861 gccgcgcgcg caaagggcga ccacgcgatc gccacggcga ccagcagcgc ggttgaaatg
     4921 aggatcagga gggtacggtg attgattgat cgatatgcat gcatgaatga agctcctagc
     4981 ggcctgttca tctgcagtat aggggagagc agcttgtggg gaaacgaaag gagcgtgaaa
     5041 acttagaggg ggttaaggtg gcccccctat tgacaacctc caaaaatcca ctatactctc
     5101 catataccca cccccctggg ggtgggggta gaatccgaca cacgagcacg atcatgaacg
     5161 actacgcccg acaggacgcc acccaacgcc tcgccagcgc cgccggccac atccgtggca
     5221 tcgagcgcat ggtgaacgac gacgcctatt gcatcgacgt catcaagcag attcaggccg
     5281 tgcaggccgc cctcagcaag gtcagcaccc tgctgctgga caaccacctg cgcacctgcg
     5341 tcaccaccgc cattcagggc gacgacgccg tcgagcgcga gcgcatgctg caggagatca
     5401 cgagcgtgtt tacggtgacg gggaagagca agtgacgagt gaagagcgtc agtggatcag
     5461 tggatcagta gatcagtaga tcagtggatc ggtagatcag tagatcggtg gttaaattct
     5521 aatttacagt aaggagtttt ttcgatggaa accaagactt tcaaagttcc gggtatcagc
     5581 tgtgggcatt gcgtccatac gatcaagatg gaagtgggcg atgtgcccgg cgtgacggcc
     5641 gttgaggccg acccgcagac caaaatggtc accgtcacct ggaatacccc cgccgactgg
     5701 gagaagatcc gcgccaccct ggtggagatc aactacccgc ccgaaggcct catttcgatc
     5761 aattagtttc cgggaaaggg ggaaagaggg aaagggagaa aggggaaaga gagaaaggga
     5821 gaaagggaga aagggggaaa gggcgttacg aacacccctt
//
LOCUS       DAOMQG010000208         5817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5828_length_5817_cov_10.769351, whole genome shotgun sequence.
ACCESSION   DAOMQG010000208 DAOMQG010000000
VERSION     DAOMQG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5828_length_5817_cov_10.769351"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1278
                     /locus_tag="PLC06_11175"
     CDS             40..1278
                     /locus_tag="PLC06_11175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO91049.1"
                     /translation="MQLSYDDLDPVTRKRGLVSVLAVTFLMNAGFFLIIPLVSVHYVD
                     RLGWAAAFIGLVLAVRQFVQQGLTVFGGALADRFGPKPLILTGIMIRTISFVVMGYAH
                     QPWVLLLSGFLAAIGGALFDAPQRATLAALAPADRLNELYGRLGILQNVARTIGPLIG
                     AYLIHFDFTMVGLGSAAFFLMSFFVILFFLPPVRVSSVTQSAGAGIRMAAGDLPFVIF
                     TVLLMGFWFMWVQLSIAMPLEIKHLTGEDSSVGLMFTISAVLAIVLQVPALRLAERFF
                     KPMPTIIAGMVVMAVGMSFVALTSSVLAFYVSLFFFSLGSVLATPSAQTVTAEMADDR
                     ARGAYFGVGSLSMAVGGGLGHILGGALVDVAAQQNTPGLPWAIFAGVGALSAVGLMIF
                     YWMIRSRPPRQLSHVPAVGD"
     gene            complement(1291..2136)
                     /locus_tag="PLC06_11180"
     CDS             complement(1291..2136)
                     /locus_tag="PLC06_11180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HRO91050.1"
                     /translation="MNFALLAINITLMLVIPVVLAIFIHRRTSASWRFFFIGAVTFIL
                     SQVLHIPFNWLVGQTGLLPDSMDTTANLLITAIFLGLSAGVFEETARYLAYRFWARDA
                     RSWSRGLMLGAGHGGIESILVGVLSAVSIIGLVVTVNNEAIMSTIPADQQAIVTESLA
                     SIMDQPWYALLLGAAERVFAITAHLAMSLMVLQVFLRRSLRWLFLSIGYHTLLNALAV
                     VVVTKAGPYAAEGALAVFSLLSLFIIFKLRTPEPEPVEPEPLPETDLPLLLPTDDAID
                     RSKYG"
     gene            2386..3024
                     /locus_tag="PLC06_11185"
     CDS             2386..3024
                     /locus_tag="PLC06_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRO91051.1"
                     /translation="MSTQRMIPAAEARVETVAGNSRFIATIGPAFTTDEAKAFIARIK
                     AEFADATHNVPAYVIGHGQTTIAHCHDDGEPSGTAGRPMLAVLQGSGLGDVVVVITRY
                     FGGTKLGTGGLVRAYGDAVREALLVVPRAEKVATVTAIAELPYTLLERARQLVEAHGG
                     VMLEESFAAEIMLTARFRSERYPAFADALRELSHGAVESMVVEENEATIMPL"
     gene            3412..5175
                     /locus_tag="PLC06_11190"
     CDS             3412..5175
                     /locus_tag="PLC06_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HRO91052.1"
                     /translation="MTAEPQVAEDSILISPYGGKLIDLMVPAAELAELRAYASTLPSV
                     RISDRSACDLELLATGAFSPLDRFMGEADHTSVVDTMRLANGILFPMPIPLPVDEEVA
                     ASLKIGQDVALRSPSNELLAIMNVEEIYPWSVEEVALKAFRTLDLRHPIVAEMHSWGK
                     FNISGPLQVLQLPARYDFKNLRLTPAETRARLEQFGHKNVVAFQTRNPLHRVHEELTK
                     RATMAKDGVLLLHPSVGMTKPGDVDHFTRVRTYKALAQRYYDQDRILLSLLPLAMRMG
                     GPREAVWHAIIRRNHGANHLVVGRDHAGPGNDSKGNPIYGPYDAQELVEKYSEELGVT
                     AVPFQMLVYLPEEDRYEEVTKVSKDTKTASISGTQVREQYLHKGKQLPEWFTRPEVAE
                     ILSETYPPRYRQGVCVWFTGLHNAGKSTTANVLTTLLQEYGRNITLLDGDVVRTQFSE
                     GLGFSREDRNDHVRRIGFVASEITRHGGIAVCAAVSPYRTTRNEVRNAIGAEHYIEVY
                     VDTPLEVCEQRDTKGLYARARRGDVVGFTGVDDPYEPPQHPELVIETLHHTPEENARM
                     ILDMLIERGFVRTDESGLRDN"
     gene            5267..>5817
                     /gene="cysC"
                     /locus_tag="PLC06_11195"
     CDS             5267..>5817
                     /gene="cysC"
                     /locus_tag="PLC06_11195"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRO91053.1"
                     /translation="MSHYEEYPGYVLWMTGLSGAGKTTIALVLEEELKNRGCKFERLD
                     GDVVRESLTRDLGFSKEDRDKNIERVSFVAKLLSRNGVGCVCSFISPYQAVRDMVRAG
                     TTNFIEVFIDAPLDVVIGRDVKGMYKKAIAGEIPNFTGISDPFEAPANPEIHIHTDRE
                     TPAESAQRILAYLEEKNYIPVREV"
BASE COUNT         1142 a         1703 c         1739 g         1233 t
ORIGIN      
        1 aatgcgccgg gcggtttttt tgtgctagac taaggggtta tgcaactgtc ttacgacgat
       61 ctcgatcctg tcacccgcaa acggggtctt gtctcggttc tggccgtgac tttcctgatg
      121 aatgcggggt tcttcctgat catccccctc gtttcggtgc attatgtgga tcgcctggga
      181 tgggccgcgg cgtttatcgg gcttgtcctg gctgtgcggc agttcgtcca acagggcttg
      241 accgtttttg gcggagcgct ggccgatcga ttcggtccca agccgctcat cctgaccggc
      301 atcatgatcc gcaccattag ttttgtcgtg atgggttatg cccatcagcc ctgggtgttg
      361 ttgctctccg gcttcctggc tgccattggg ggtgcgctct tcgacgctcc acagcgggcc
      421 acgctggccg cattggctcc ggccgaccgg ctcaatgagc tttacggccg cctgggcatt
      481 ttgcaaaacg tggcccggac catcggcccg ttgatcggcg cgtacttgat tcatttcgat
      541 tttacgatgg tgggacttgg gtcggccgct tttttcctga tgtcgttctt cgtgattttg
      601 tttttccttc cgccggtcag ggtgtcttcg gtcacccaat ctgccggggc cggcatcaga
      661 atggccgccg gcgatctgcc gttcgtcatc ttcaccgtcc tgttgatggg cttctggttc
      721 atgtgggtgc agttgtccat cgccatgccg ctggagatca aacacctgac cggcgaagac
      781 agcagcgttg gcctcatgtt cacgatcagc gctgtgctgg cgatcgtcct gcaggttccg
      841 gcgctacgcc tggctgagcg tttcttcaag ccaatgccga cgatcatcgc cgggatggtg
      901 gtcatggcgg tagggatgag cttcgtcgcc ctcacgtcga gtgtgctcgc cttctatgtc
      961 tcgctgttct ttttctcgct cggctctgtc ctggccacgc ccagcgccca gactgtgacg
     1021 gccgagatgg ccgacgaccg cgcgcgcggc gcgtatttcg gcgttggttc gctgtctatg
     1081 gctgtgggtg gtggcctggg ccatatcctg ggcggagcac tggttgatgt ggccgcgcaa
     1141 caaaacacgc cgggcttgcc gtgggctatt ttcgccgggg tgggggcgct gtcggccgtc
     1201 gggctgatga tcttctactg gatgatacgt tcgcgaccac cacgacagct atcgcacgtg
     1261 cctgcggtgg gcgactaggc gcgctctcga ttaaccgtat ttgcttcgat cgatagcatc
     1321 atctgtcggt agcaacaaag ggagatctgt ttccggcaat ggttccggct cgacaggttc
     1381 gggttccggc gtccgcagct tgaagatgat gaacaggctg agcaggctga atacggccag
     1441 cgccccttcg gccgcatagg ggccggcctt ggtgacgacg accacggcta gtgcgttgag
     1501 cagcgtatgg tagccgatgg acaggaatag ccagcgcagg gatcgtcgca ggaagacctg
     1561 gagcaccata agcgacatcg ccagatgggc ggtgatggcg aagacgcgct cggccgcgcc
     1621 cagcagaagc gcgtaccacg gctgatccat gatgctcgcc aggctctcgg tgacgatagc
     1681 ttgctgatcg gcggggatgg tgctcatgat ggcttcgttg ttgactgtca ccaccaaccc
     1741 gatgatgctg acggccgaca gcacgccgac cagaatcgac tcgatgccgc catgccccgc
     1801 gccgagcatc agaccgcgcg accacgagcg ggcatccctg gcccagaaac gataggccag
     1861 atagcgggcc gtttcctcaa agaccccggc cgataacccg aggaagatcg cggtgatcag
     1921 caggttggcg gtagtatcca ttgagtccgg caacagcccg gtttggccga ccagccagtt
     1981 gaacgggata tggaggactt gcgagaggat gaaagtgacc gcgccgatga aaaagaagcg
     2041 ccagctcgcg ctcgtgcggc gatggataaa tatcgccaac acgacaggaa tcacgagcat
     2101 cagagtgata ttgatagcga ggagggcgaa gttcataggc acaataatac caccgtccgg
     2161 tagaggtgtg gagtgcagct aatcccactc attttcggtt gatggggtaa ccccacgagg
     2221 ttaccgaatc caaacaagcc gtcttttctt tagatagttt gactcgcgac gctctaccgg
     2281 agcagtggcg ttgattggat aatagcaaat tccggctgcc caaagatagt gataaatgtc
     2341 accccaatca catgacattt tcgcggcaag gcgtagaatg acagaatgag cactcaacgg
     2401 atgatccccg cggccgaggc gcgcgtcgag acagtcgcgg gcaattcgcg attcattgcc
     2461 accatcggtc cggccttcac gacggacgag gccaaagcct ttatcgcccg aatcaaggct
     2521 gaattcgctg acgccaccca caatgtcccc gcttacgtca tcggccatgg gcagacaacg
     2581 atcgcccatt gccacgatga cggcgagccg tccggcacgg ccgggcggcc gatgctggcc
     2641 gtgttgcagg gcagcggcct gggtgatgtc gttgtggtga tcacccgcta ttttggcggg
     2701 acaaagctgg ggacgggcgg gcttgtgcgc gcctatggcg atgcggtgcg cgaggcgctc
     2761 ctggtcgttc cccgcgccga gaaggtggcc accgtcacgg ccatcgccga actgccgtac
     2821 acgctgctgg agcgcgcgcg acaactcgtg gaggcgcatg gaggcgtgat gctggaagaa
     2881 tctttcgccg ccgagattat gctgactgcc cgttttcgca gcgagcgcta tcctgctttt
     2941 gccgacgcgc tgcgcgagct gtcccacggc gctgtcgaat ccatggtggt tgaggagaac
     3001 gaggcgacga ttatgcctct ttgatgaatg tcttggtcaa cacgcgatag ttggcctggt
     3061 cgtggatata atccgctaaa tgttaatctt aatcgtaaga ttacttcgct agattagaac
     3121 ccagagcaac acagccgaac gtttacgcat tgctaaagcg gtcacattgt cgtccgcgct
     3181 ataaatcccc ttcagttgga ctgaacgcgt tgcaagagag tttcgcccgc gtggtctttc
     3241 ccgaccgacg tatgggcttg tgtcggcgaa gcggtgataa agcgatcgat ctgtgtcgac
     3301 ttgattattg gaataaactt gtaaatttta aataaggcgc gccgtcattc gaactcgatt
     3361 cgaaccacac aatcccgcgg cgcggtcaag gaaaggtgtg aattgttaat gatgactgca
     3421 gagcctcaag tggccgaaga ttcgatcctg atttctccct acggggggaa gctcattgac
     3481 ctgatggtcc ccgcggccga gctggccgag ctgcgcgctt acgccagcac gctcccatcc
     3541 gtgcgaatct cggacaggtc tgcttgcgac ctggaattgt tggccaccgg cgctttttcg
     3601 ccgctcgatc gcttcatggg tgaagcggat cacacgagcg tggtcgatac gatgcgcctg
     3661 gcgaacggta ttctgttccc catgcccatc ccgctgcccg tcgatgagga agtcgcggct
     3721 tcgctcaaga ttggtcaaga tgttgccctg cgcagcccca gcaacgagtt gctggcgatc
     3781 atgaatgtag aagagatcta tccatggagc gttgaggaag tggccctgaa ggctttccgc
     3841 acgcttgacc tgcgccatcc catcgtcgcc gagatgcata gctggggcaa gttcaatatc
     3901 tccggcccac tgcaggtcct gcaattgccg gcgcgctatg atttcaaaaa tttgcgcctg
     3961 acccctgccg agactcgcgc tcgcctggag caattcggtc acaagaacgt cgtcgccttc
     4021 cagacccgca acccgctgca ccgcgtgcac gaagagctga ccaagcgggc gacgatggcg
     4081 aaggacggcg tgttgctgct gcatcccagc gtcggcatga ccaagcccgg cgatgtcgat
     4141 cacttcactc gcgttcgcac ctacaaggcg ctggcccagc gatactatga ccaggaccgt
     4201 atcctgctgt ccctgctgcc gctggccatg cgcatgggcg gcccgcgcga ggccgtctgg
     4261 catgcgatca tccgccgcaa tcatggcgcc aatcacctgg tcgttggtcg cgaccatgcc
     4321 ggccccggca acgattcgaa gggtaacccg atctatggcc cctacgatgc gcaggaactg
     4381 gtcgagaaat atagcgaaga actgggcgtg acggccgtgc cgttccagat gctggtctac
     4441 ctgccggaag aagatcgcta cgaggaagtg accaaggtct ccaaggacac gaagactgcc
     4501 tcgatcagcg gtactcaggt gcgcgaacag tacctgcata agggcaagca attgcctgag
     4561 tggttcactc gtcccgaagt agccgagatc ctgtctgaga cctatccgcc acgctatcgc
     4621 cagggagttt gcgtctggtt taccggcctg cacaacgccg gcaaatccac gaccgctaac
     4681 gtgctgacga cgctgctcca ggagtacggg cgcaacatca ccctgctcga cggtgatgtc
     4741 gttcgtaccc aattctccga aggtctgggc ttcagccgcg aagatcgtaa cgatcacgtg
     4801 cgccgcatcg gctttgtcgc ctcagagatc acacggcatg gcgggatcgc cgtctgcgcc
     4861 gccgtcagcc cctatcgcac cacgcgaaat gaagtgcgga acgccatcgg cgccgagcac
     4921 tacatcgagg tctatgtcga tacgccgctc gaagtttgtg agcagcgcga cacgaagggt
     4981 ctctatgccc gcgctcgccg cggtgatgtc gtcggtttca ccggcgtcga tgatccgtat
     5041 gagccgccgc agcaccccga gctggtcatc gagaccttgc accacacgcc cgaagaaaac
     5101 gcgcggatga tccttgacat gttgattgag cgcggtttcg ttcgcacgga cgagtcgggc
     5161 ttgcgcgaca attaatcaaa aaatcaatat tcagcaggcc ggtattcagt gcaaactgtg
     5221 ccggcctgcc ggccattcat tgaaaaacaa gaggaaaata tacgaaatgt cccattacga
     5281 agaatacccc ggctacgtcc tgtggatgac cggcttgtcc ggcgcgggca agaccaccat
     5341 tgccctggtt ctggaagaag agttgaagaa ccgcggctgc aaattcgagc gtctcgacgg
     5401 tgatgtcgtg cgcgagagcc tgacccgtga cctgggcttc agcaaggaag accgcgacaa
     5461 gaacatcgag cgtgtctcct tcgtcgccaa gctgctgtcg cgcaatggcg ttggctgcgt
     5521 ttgctcgttc atttcgccct atcaggccgt ccgtgatatg gtgcgtgccg gcactaccaa
     5581 cttcatcgag gtcttcatcg acgcgccgct ggatgtcgtc atcggccgcg atgttaaggg
     5641 tatgtacaaa aaggctatcg ccggcgaaat ccccaacttt accggcatct ccgatccgtt
     5701 tgaggctccg gccaaccccg agatccacat ccacaccgac cgggaaaccc cggccgagag
     5761 cgcgcagcgc atcctggcct atctggaaga gaagaactac attcccgtcc gcgaagt
//
LOCUS       DAOMQG010000209         5773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5883_length_5773_cov_8.859391, whole genome shotgun sequence.
ACCESSION   DAOMQG010000209 DAOMQG010000000
VERSION     DAOMQG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5883_length_5773_cov_8.859391"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLC06_11200"
     CDS             complement(<1..182)
                     /locus_tag="PLC06_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91054.1"
                     /translation="MDLQSEPFLLHDVVRRQSKDGRTFLLTTLRDRTGQISGVFWDVP
                     LTVDVWVRPGLAVLVSG"
     gene            520..1104
                     /locus_tag="PLC06_11205"
     CDS             520..1104
                     /locus_tag="PLC06_11205"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013574563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRO91055.1"
                     /translation="MKKIIVGLSGASGVIYGIRLLEVLQALPDVESHLVMSTAAATTI
                     PLETDYTPEEVRALATADYRFKDIAAAISSGSFRTDGMVILPCSMKTLSAIAYSYSDN
                     LLTRAADVVLKERRKLILCPRETPLHLGHLRAMTLAAEIGAHIVPPMPAFYHRPQTVD
                     DIINQTVNRVLDLLDITLPEDLFRRWQGGRSAER"
     gene            1179..1625
                     /locus_tag="PLC06_11210"
     CDS             1179..1625
                     /locus_tag="PLC06_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRO91056.1"
                     /translation="MTDTRSLALDYLNSHHVLTLATHGSEGLWAAAVFYASAGFDLYF
                     LSAGHTRHARNIATSPHVAATIQEDYADWSAIKGIQLEGAVRLLAGREREGAIATYTK
                     KFSFLAQPVAVIEAALSRVNWYCLSPSRLYFVDNSRGFGHRDEVEL"
     gene            complement(1622..2065)
                     /locus_tag="PLC06_11215"
     CDS             complement(1622..2065)
                     /locus_tag="PLC06_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRO91057.1"
                     /translation="MQPNIIRPIVICVFQHNGCILAAEGHEPLKGQAFYRPLGGGIEF
                     GEYSADAIKREISEELGTAVVGLRYLGTIENVFAYNGQTGHEIVMVYDGSFADRSLYE
                     QPFIEALENDGSLFRAVWVNLSDCAKPGAPLLYPTGLLEMLNAQR"
     gene            complement(2078..2641)
                     /locus_tag="PLC06_11220"
     CDS             complement(2078..2641)
                     /locus_tag="PLC06_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO91058.1"
                     /translation="MDMSTVIRKRFTAEEYEQIAAAGILGEDERFELLEGEIVAMSPL
                     GPQHSATVTRLTELFYELGISDITIRVQDPIRLGDYSSPQPDVVVVNRRSDRYAGGHP
                     EPEDILLLIEVSESSLAYDRDVKLPLYARAGIAEVWLVALLPQVVEVYRAPNDNGYGE
                     KRTFHRGDILTPLHLSGVGLPVERILG"
     gene            complement(2665..4758)
                     /locus_tag="PLC06_11225"
     CDS             complement(2665..4758)
                     /locus_tag="PLC06_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91059.1"
                     /translation="MQLQDLTPLQQEAAAANGASWLYGPAGTGKTTALHQRLLRLLRE
                     GEPAYTILTLVAEPEHRAAFFDTVHQSGLGPYAELNVTDYTRLAGSMVALFWPLVARD
                     AGFDRPYLPPTPLSYDQAQVLMWRVITPMLADGAFANLRLRPQQIVSQVLDTLNRAAL
                     NALSLEEAIARQSRTWVGEPERRHHLDDAATAARTFRRHCLANSLLDLSLTVEVFDTQ
                     LVRHPEFHRYFRERYRHLIVDNLEEQTPAGQNFVAGLMGETQTTAVAVDAGGGYKRFL
                     SADPHGAEGMRHRFTRIFDFTESFVAPADMGLLANQVENYLMHRALPAAGAKTRILDV
                     IGGRYRREMVANLGPALHKLVYEEGVAPRDIAIIVPYLDGALRYMLTNALREAGLPFR
                     QLRRRTSPREEPRVRAWLTWLALAHLGWGVHPAPYDVAEALTLSIHDLDPARAQLVVD
                     NLYRPDAPELLPVDSLSSRIVERVGEEPLRLVEALRLWLAENGHRDTLDTFLHRLFNE
                     LLAQPQFQPEPDRAGAAVLDWLVRSAARLRKAAPGMGLMTEADIGATFIDAVNQGLVT
                     ANPPDWGDPPDPEGVVLSTIYAYLLAGEPARVQVWLETAAQGWWDIPRQPLSNAFVLA
                     QSRSPELPWTIDEEFAVRNELLTRIVRGLTARCRDGIILATSDLDRRGVRQDGPLWRA
                     LGPLVRERDHASNEV"
     gene            complement(4869..>5773)
                     /locus_tag="PLC06_11230"
     CDS             complement(4869..>5773)
                     /locus_tag="PLC06_11230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO91060.1"
                     /translation="ISTAAPNVVLDVPRESRTLTVPDLKKPITLQLSADVSWLDNETR
                     EIVAAQLLVNDVQAAEIPPGDLKSFRASIPNFAIGDNRLEVVVVDNQGITSTSAPVII
                     TVAEGERLEVPEALQPGGISFSWTWLLWLVAMAGLAAVGFWLWRSRSKGATGEPKSRR
                     RRRGSRPPVTTAPPQDATDDFDADAGLDFLRSEGYEAPFVMAHLEVVDAQTLMPDELN
                     LGEAEVRIGRSPQQSHIAFRDDITVSRYHAVMRLEGNRYRIYDAGSTSGTYVNGRQVP
                     EYGLQLADGDEIQLGAVRMHYRQL"
BASE COUNT         1103 a         1768 c         1834 g         1068 t
ORIGIN      
        1 ccgctgacca gcacggccag ccccggccgc acccatacat cgacggtcag cggcacgtcc
       61 caaaaaacgc cgctgatctg cccggtgcga tcgcgcaggg tggtcagcag gaaggttcgg
      121 ccgtctttcg attgccgccg gacgacgtca tggagcagga acggctcgct ctgtaggtcc
      181 atccccgctt tcaggtcacc gatatatctt gatttctctg tcacgcacac gctcgatttt
      241 ggaatttccc ggcgatcccc tacaatgcat tgtgggatac cggaagggca attatacccc
      301 caacggcttt ccctgcgaga cgcgacaaga ctattggata tatccgatca tagggggaat
      361 agaacgcgga tgacacgggt tgaacggatt cacgcggatc aatcgttgtt ctgatctatg
      421 ttaatccgcc tgatccgcgt catcagcgtt ctattctttt agttacaatt accctgcaag
      481 gatgagcctg gacgtggcct cataaaaggc aaggacatta tgaaaaagat catcgtcggt
      541 ctatcgggag ccagcggcgt catctacggc atccggctgc tggaagtgtt gcaggcgctg
      601 ccggatgtgg aatcgcatct ggtgatgagc acggccgcgg cgaccaccat cccgctggaa
      661 acagactaca cgccggaaga agtgcgggcg ttggcgacgg ccgattatcg cttcaaggat
      721 atcgccgccg ccatctcgtc cggctcgttt cgcaccgacg gcatggtcat cctgccttgc
      781 tcgatgaaga cgctgtcggc catcgcctat tcatacagcg acaatctgtt gacgcgggcg
      841 gccgacgtgg tgttgaagga acggcgcaag ctgatcctct gcccgcgcga gacgccgctg
      901 catctgggcc atctgcgggc catgacgctg gcggccgaga tcggggcgca tattgtgccg
      961 cccatgcccg cgttctatca tcggccgcaa acggtcgacg acatcatcaa ccagacggtc
     1021 aaccgcgtgc tcgatctgct ggatatcacc ttgcccgaag atttgttccg gcgctggcag
     1081 ggggggcgga gcgcggagag atgacgacgg acgacgggta ctcttggtca gtggtcagtg
     1141 gtctgtgacc cattaacctc catcattgaa ccttcaccat gaccgacaca cgcagtctcg
     1201 cgcttgatta tctgaacagc catcatgtgc tgacgctggc gacgcatggg tcggaggggt
     1261 tgtgggcggc ggccgtgttc tatgcgtccg ccggcttcga tctctacttt ctctcggccg
     1321 ggcacacgcg ccacgcgcgg aatatcgcca catcgccgca cgtcgcagcc acgattcagg
     1381 aggactacgc ggactggtcg gccatcaagg gcatccagtt ggagggagcg gtgcggctgt
     1441 tggcggggcg ggagcgggaa ggggcgatcg ccacctatac gaagaagttt tcgtttctgg
     1501 cccaacccgt tgctgtcatc gaggcggctt tatcgcgggt gaactggtat tgcctgtcgc
     1561 cgtcacggtt gtactttgtg gataatagtc gggggtttgg gcatcgggat gaggttgaac
     1621 tctagcgttg ggcgttcaac atttcaagca gccccgtcgg ataaagcagc ggcgcgccgg
     1681 gtttggcgca atcgctcaaa ttgacccaca ctgcgcgaaa cagcgatccg tcgttttcca
     1741 gggcctcaat gaacggctgc tcgtagagcg agcggtcggc gaatgacccg tcgtagacca
     1801 tgacgatctc gtgacccgtc tgaccgttgt aggcgaagac attctcgatt gtgcccaggt
     1861 agcgcagacc gacgacggcc gtgcccagct cctcgcttat ttcacgctta atggcgtcgg
     1921 cgctgtattc gccgaactcg atgcctccac ccagcggccg atagaatgcc tgccccttga
     1981 gcggctcatg gccttcggcg gccaggatgc aaccattgtg ttgaaagacg cagataacga
     2041 tggggcggat gatgttgggt tgcatcaagg tacaagctca accgagaatg cgttctacgg
     2101 gcaacccgac ccccgataaa tggagcgggg tcaatatatc gccgcgatga aaagttctct
     2161 tctcaccata accattatca ttaggtgcgc gatagacctc tactacctgc ggcaacagcg
     2221 ccaccagcca cacttcagcg atgcctgcac gggcgtaaag cggcagcttc acgtcgcgat
     2281 cataagccaa agacgactca gaaacctcaa tgagaagcag aatatcttcc ggctcagggt
     2341 gaccacctgc atagcggtcg ctacgtcgat tgacaaccac aacatcaggc tgtggggacg
     2401 aataatcacc aaggcgtatc ggatcttgca cccgaatcgt tatatctgaa attcccagct
     2461 cataaaataa ctccgtcagg cgagtgacgg ttgccgaatg ttgcgggcct aatggactca
     2521 tcgccacgat ctccccttcc agtaactcaa atcgctcatc ctcgcctaaa ataccggcag
     2581 cggcgatctg ttcatattcc tcggcggtga aacgtttacg aataacggtc gacatgtcca
     2641 ttgattcggt tctcctgcca gttattatac ctcattcgaa gcatgatcac gctcacgcac
     2701 caacggcccc aacgcccgcc acaacggccc gtcctgccgc acgccgcggc ggtcgaggtc
     2761 gctggttgcc aggataatcc cgtcgcggca gcgggccgtc aggccgcgca cgatgcgcgt
     2821 cagcagttcg ttgcgcacgg cgaactcctc gtcgatcgtc cagggcagtt cgggcgaacg
     2881 gctctgggcc agcacaaagg cgttgctcag cggctggcgg gggatatccc accaaccctg
     2941 cgcggccgtc tccagccaca cctgcacccg cgccggctcc ccggccagca agtaggcgta
     3001 gatcgtggag aggacgacgc cctcggggtc gggcggatcg ccccagtcgg gcgggttggc
     3061 cgtcaccagc ccctgattga cggcgtcgat aaacgtcgcg ccaatgtcgg cttcggtcat
     3121 cagccccatc cctggcgcag ctttgcgcag ccgggcggcc gaacgcacca gccagtcgag
     3181 cacggccgcg ccggctcgat ccggttccgg ctgaaattgg ggctgggcca gcaactcgtt
     3241 gaacaggcga tgcaggaagg tatcgagggt gtcgcggtgg ccattctccg ccagccagag
     3301 gcgtaatgcc tccacaagcc gcaacggttc ctcgcccacg cgctcgacga tgcgcgacga
     3361 caggctatcg acgggcagca gctccggtgc gtcgggtcgg tagagattgt caaccaccag
     3421 ttgcgcgcgg gccgggtcaa ggtcatggat gctgagcgtc agcgcctcgg ccacatcgta
     3481 gggcgcgggg tggacgcccc agccgagatg ggccagcgcc agccacgtga gccaggcgcg
     3541 cacgcgtggc tcctcgcgcg ggctggtgcg gcggcgcaac tggcgaaacg gcagcccggc
     3601 ctcgcgcaag gcgttggtca gcatgtagcg cagcgcgcca tccaggtagg ggacgatgat
     3661 ggcgatgtcg cgcggggcga cgccttcctc atagaccagc ttgtggagcg ccgggccgag
     3721 attggccacc atctcgcgcc ggtagcggcc gccgatgacg tccaggatgc gcgtcttggc
     3781 tccggctgcc ggcagcgccc tgtgcatcag ataattctcg acctgattgg ccaacaggcc
     3841 catatccgcg ggggccacga acgactcggt gaagtcgaag atgcgggtga agcgatggcg
     3901 catgccctcc gcgccgtggg gatcggccga aagaaaacgc ttgtagccgc cgccggcatc
     3961 cacggccacg gccgtcgtct gcgtctcgcc catcagcccg gcgacgaagt tctgcccggc
     4021 gggggtctgc tcctccagat tgtcgacgat gagatggcga tagcgctcgc ggaaatagcg
     4081 gtggaactcg gggtgtcgca cgagttgtgt atcgaacacc tcgacggtca gcgacagatc
     4141 gagcagactg ttggccagac aatggcggcg aaaggtgcgg gcggccgtcg ctgcgtcgtc
     4201 gaggtggtgg cgacgctccg gctcgccgac ccaggtgcgc gattggcgag cgatggcttc
     4261 ctccagcgac aaggcattga gcgcggcccg attgagcgtg tccagcacct ggctgacgat
     4321 ctgctgcggc cgcaggcgca aattggcgaa cgccccgtcg gccagcatcg gcgtgatcac
     4381 gcgccacatg agcacctggg cctggtcgta gctgaggggt gtcggcggca ggtaggggcg
     4441 atcgaacccg gcatcgcggg ccaccagcgg ccagaacagg gccaccatgc ttcctgccag
     4501 ccgggtgtag tcggtgacgt tcagctcggc gtaggggccg agaccggact gatggacggt
     4561 gtcgaaaaag gcggcgcggt gttccggctc ggccacgagg gtcaggatgg tataggccgg
     4621 ctcgccttcg cgcagcagcc gcagcagccg ctgatgcagc gcagtggttt tacccgtgcc
     4681 cgccgggccg tagagccagg aagcgccgtt ggccgcggcc gcctcttgct gaagtggggt
     4741 caggtcttgc aattgcatca actaattgta gcgccggttg cttaaccggc gatgagcaac
     4801 cggcccgcat ctccgcaggt agcggaggtt tcctaaccaa cggaggacgt ccggttagga
     4861 aaccggcgtt acagttgacg atagtgcatc cgcaccgcgc ccagctggat ctcgtccccg
     4921 tcggccagtt gcagcccata ttccggcacc tggcggccgt tgacgtaagt gccgctggtg
     4981 ctgcccgcgt cgtagatgcg atagcgattg ccttccagcc gcatcacggc gtgatagcgc
     5041 gacacggtga tgtcgtcgcg aaaggcgatg tgggattgct ggggagagcg gccgatgcgc
     5101 acttcggctt cgccaaggtt cagttcatcg ggcatgaggg tctgcgcatc caccacttcg
     5161 aggtgggcca tgacgaacgg ggcctcatac ccttcggagc gcagaaaatc gagtccggca
     5221 tcggcatcga agtcatcggt cgcgtcctgc gggggggcgg tcgtcaccgg cggccgcgac
     5281 ccgcgacgac gacgacgtga tttaggctcc ccggtggcgc ccttcgaccg gctgcgccac
     5341 agccagaagc ccaccgcagc caaaccggcc atcgccacaa gccacaagag ccacgtccaa
     5401 ctaaacgata tgccgccggg ttgcagcgct tccggcacct caagtcgctc gccttcggcc
     5461 acggtgatga tgaccggcgc gctggtcgag gtgataccct ggttgtcgac gacgaccact
     5521 tccagccggt tgtcgccgat ggcgaaattg gggatcgagg cgcggaagga tttgagatcg
     5581 ccgggcggga tttcggccgc ctggacatcg ttaaccaaca attgcgccgc cacgatctcg
     5641 cgcgtttcat tgtcgagcca ggatacgtcg gccgacagtt gcagagtgat cggtttcttt
     5701 agatcgggca cggtcagagt gcgactctcg cgcggcacgt cgaggacgac attgggcgcg
     5761 gcggtggaga tga
//
LOCUS       DAOMQG010000210         5748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5907_length_5748_cov_9.855963, whole genome shotgun sequence.
ACCESSION   DAOMQG010000210 DAOMQG010000000
VERSION     DAOMQG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5907_length_5748_cov_9.855963"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLC06_11235"
     CDS             <1..240
                     /locus_tag="PLC06_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91061.1"
                     /translation="GRHVHIWQEGESYHILVVSRDPAVAAALRELARIESECRLHPGS
                     QPELIDDHEGYLLIAHGPNAFIFARLFLALEAETP"
     gene            325..2616
                     /locus_tag="PLC06_11240"
     CDS             325..2616
                     /locus_tag="PLC06_11240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRO91062.1"
                     /translation="MNTPDTELRFKITGMDCASCAKSIEKGVAGLPGVEGATINFTTG
                     TLRVAGAAAPERVVARVRELGYDVALGEGEKGRRGEGESGGHAHPDHESPAQKSGGLI
                     SYLWNRTDTRLALLAALLVIPGILFDELLPGLGIESPVFAAMSVLAMIIAGYPVARSA
                     LTSLRINREITINLLMTIAAVGAVIIGAYTEAGLVMVLFALSEGLEGYTANRARDAIG
                     SLSELAPATATAVRDGVETPIPVEALRVGDHIVVKPGERLAMDGRVLAGVSAVNQAPI
                     TGESRPVDKAAGDEVFAGSVNGHGALEVEVTHLAEDNTISRMIRLVADAQEGRAPAQR
                     FVDRFARVYTPLVVFIAFLVATIPPLFFGEPFWNPAPDVQGWFYKALALLVVACPCAL
                     VISTPVTLISAISNGARQGVLFKGAVFLEELSRVRAIGFDKTGTITRGQPAVVGVHAA
                     DCGEEITNYELRITNGEIGGQACEACDDVLALAAAVERRSEHPLAGAVMAEADARGVS
                     GRYASTDVAALTGRGISGQVNGRTVTVGSHAFFEDDATHDPFHDRIAAADAQGLTTVL
                     VSEDDAYRGYIAMADTTRAGAAEAMADLSKMGISSLVMLTGDHAATAERIAAEVGLTD
                     VRASLLPADKVAAVEELRAKYGNVAMVGDGINDTPALAAASVGLALGRTAQALETADV
                     VLLGDDLRQLPFAVRLARMAMNTVRVNVALSIGIKVVFFVLVLIGSGSMWMAVLADLG
                     TSLLVTANGMRLLRRPKLELGAG"
     gene            2759..3022
                     /locus_tag="PLC06_11245"
     CDS             2759..3022
                     /locus_tag="PLC06_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008184183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRO91063.1"
                     /translation="METTKLSSKGQIIIPKWLRDAYRWDAGLEFVVIDTGEGILLRPS
                     RPFEPATLDEVAGSLPSSGKSRTVEEMAADLQRGIREARDDRG"
     gene            3009..3401
                     /locus_tag="PLC06_11250"
     CDS             3009..3401
                     /locus_tag="PLC06_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO91064.1"
                     /translation="MIAVDTNVVVRLLVLDDPDQSARARDLFAAEQVFIPDTVVLETA
                     WVLAYSYELGKDAIAVGLRRLFGLPNVHLRDAAEMALALEWYGRGLDFADALHLVQSA
                     HCSRLLTFDRRFVARGAGISGVAVALVG"
     gene            3527..>4215
                     /locus_tag="PLC06_11255"
     CDS             3527..>4215
                     /locus_tag="PLC06_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRO91065.1"
                     /translation="MALTHVPTRSEVDPRHTWNAESVFATVDEWAAELAALSAGLGEL
                     DRFKGHLGDGPDVLADALESTQAYGQRVEMLLIYTALTESVDRSDQAATVRAGQAGSL
                     YGQVAGATAYIEPELLAIGQDRLTEWVATEPRLSQYAHYFDTLFRRQAHVRSSEVEEV
                     IGLAADAFGGTRSTFSKLTDSDFKFRPVTLPGQDDLPVTQGTAGDLIQSPDRDVRRIA
                     WENYHDQYLAY"
     assembly_gap    4216..4315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4316..5092
                     /locus_tag="PLC06_11260"
     CDS             <4316..5092
                     /locus_tag="PLC06_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRO91066.1"
                     /translation="GCLDERWVDIYPNEGKSSGAFSWGCQGTYPFIVMSYTDDANSMG
                     TLAHELGHSMHSFLSWENQPHIYSNYSLFAAEVASNFNQAMLRAYLLEQDIDRQLKIA
                     VLEEAMSNFHRYFLEMPTLALFELEMHRREERGQGLTADDMIAYMADLFAETYGDAAA
                     VDPQRDGIRWATFGHLYADYYVYQYATGISGANALARRVLSGQSGAAADYLGFLSAGG
                     SRYPLEALRSAGVDLTQPAPVEAAFEVLGGLIDKLDALVN"
     gene            5166..5708
                     /locus_tag="PLC06_11265"
     CDS             5166..5708
                     /locus_tag="PLC06_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRO91067.1"
                     /translation="MRRSPARIVVVGTTGSGKSTLAERLAARIGGDFVDLDALNWGPD
                     WTPAPVPEFRERATAALAAECWVVAGNYSKLRDIVWGAADTLIWLDYPLPLVLWRLLR
                     RTIRRVATREELWNGNRERFREQFASRESLFLWAIKTYSRRRHEYPAALALPEYAHLR
                     VLRFRWPRQMERWFNSMSNR"
BASE COUNT          941 a         1763 c         1879 g         1065 t
ORIGIN      
        1 ggccggcacg tccacatctg gcaggagggg gagagctacc acatcctcgt cgtcagccgc
       61 gatccggcgg tggccgccgc cttgcgggaa ctggcgcgca tcgaatcgga gtgccgcctg
      121 catcccggct cgcagcccga actcatcgac gaccacgagg gctacctgct catcgcccac
      181 ggccccaacg ctttcatctt cgcccgtctg tttttggcat tggaagccga aaccccttga
      241 cattcaactc gacttgaagg tgtatcatcc atgtagcgcc ggtttcttaa ccggcgagtt
      301 ggaatattga atccggctat tgttatgaat acacctgata ccgaactacg tttcaaaatc
      361 accggcatgg actgcgccag ttgcgccaaa tccatcgaga agggcgtcgc ggggctgccc
      421 ggcgttgaag gggcgacgat caacttcacc accggcaccc tgcgcgtcgc cggcgcggcc
      481 gcccccgagc gcgtcgtcgc ccgcgtgcgc gagttggggt atgacgtggc tttgggagaa
      541 ggggagaagg ggagaagggg agaaggggag agtggcggcc atgctcaccc ggatcacgag
      601 tctcccgcac agaaatctgg cggcctgatc agttacctct ggaaccgcac cgacacgcgc
      661 ctggccttgc tggccgcgct gctggttatc cccggcattc tgttcgatga gctgctgccc
      721 ggcctgggaa tcgagtcgcc cgttttcgcc gccatgtcgg tgctggcgat gatcatcgcc
      781 ggataccctg tcgcccggag cgcgctgacc agcctgcgca tcaatcgcga gatcacaatc
      841 aacctgctca tgaccatcgc cgccgtcggt gcggtcatca tcggcgccta caccgaggcc
      901 gggctggtga tggttctgtt cgccctgagc gaggggctgg agggctacac cgccaaccgc
      961 gcccgcgacg ccatcggcag cctgtcggaa ctggctccgg ccacggctac ggccgtccgc
     1021 gacggggtgg aaacgcccat tcccgtcgag gcgctgcgcg tgggcgacca tatcgtcgtg
     1081 aagcccggcg agcgtctggc gatggacggc cgcgtgctgg ccggtgtgtc ggccgtcaat
     1141 caggcgccca tcaccggcga gagtcgcccg gtggacaagg ccgccggcga cgaggtgttc
     1201 gccggcagcg ttaacggcca cggcgcgctg gaggtcgagg tgacccacct ggctgaggac
     1261 aacaccatca gccgcatgat tcgtctggtg gccgacgctc aggagggccg cgccccggcc
     1321 caacgcttcg tcgaccgctt tgcccgcgtc tacacgccgc tggtggtctt catcgccttc
     1381 ctggtggcga ccatcccgcc gctcttcttc ggcgaaccgt tctggaatcc cgcgccggac
     1441 gtgcagggct ggttctataa ggcgctggcc ttgctggtcg tcgcctgccc ctgcgcgctg
     1501 gtcatcagca cgccggtgac gctcatcagc gccatcagca acggcgcgcg tcagggcgtc
     1561 ctcttcaagg gcgcggtatt tctggaagag ttgagccgcg tgcgggctat cggctttgac
     1621 aagacgggca cgatcacccg cgggcagccg gcggttgtgg gcgttcatgc ggcggactgc
     1681 ggggaagaaa ttacgaatta cgaattacga attacgaatg gggagatcgg cggtcaggct
     1741 tgtgaagcct gcgacgacgt gctggctttg gccgcggccg tggagcggcg cagcgagcat
     1801 cccttggcgg gggcggtgat ggctgaggcc gacgctcgcg gggtcagcgg tcgctacgcg
     1861 tcgaccgacg tggccgcgct aaccggccgc ggcatcagcg gccaggtcaa cgggcgcacc
     1921 gtcaccgtcg gcagccacgc tttcttcgag gacgacgcta cgcatgaccc cttccatgat
     1981 cgcatcgccg ccgccgacgc ccaggggttg acgacggtgc tggtgagcga ggatgatgcc
     2041 tatcgcggct acatcgcgat ggccgacacc acgcgggcag gtgcggccga agcgatggcc
     2101 gatctgtcga agatgggcat cagttcgctg gtgatgctga ccggcgacca cgccgccacg
     2161 gccgagcgca tcgccgccga ggtggggctg actgacgttc gcgcgagctt gcttccggcc
     2221 gacaaggtgg ccgccgtcga ggagttgcgc gcgaagtacg gcaatgtggc catggtcggc
     2281 gacggcatca acgacacgcc cgcgctggcc gcggcttcgg tcgggctggc gctggggcgc
     2341 acggcacagg cgctggagac ggccgacgtg gtgctgttgg gcgacgattt gcggcagctg
     2401 ccgttcgccg tccgtctggc gcgcatggcc atgaacacgg tgcgcgtcaa tgtggcgctg
     2461 agcatcggca tcaaggtcgt gttcttcgtg ctggtgctga tcggcagcgg cagcatgtgg
     2521 atggccgtgc tggccgacct gggcacatcg ctgctggtga cggccaacgg gatgcggctg
     2581 ttgcggcggc cgaagctgga gctgggggcg gggtagcggt ctgcctgtgg cgctacctgt
     2641 caggttgcgt tccgcagatc aacaagctgg acagcttgtt ctacatccct gcgatgtcgg
     2701 ttgatttcat tctgctttcg gattataatc ttacaaaatt cttaccgagg cgagccgcat
     2761 ggaaacgacc aagctatcga gcaaaggaca gatcatcatc cccaaatggc tgcgcgacgc
     2821 ctatcgctgg gatgccggac tggagtttgt cgtcatcgac accggcgagg gtattttgct
     2881 gcggcccagc cggccgtttg agccggcgac cctcgacgaa gtagccggaa gcttgcctag
     2941 tagcggtaaa tcgcgcaccg tcgaggagat ggcggccgat ctacagcggg gcattcggga
     3001 ggcgcgggat gatcgcggtt gataccaacg tcgtcgtgcg gctgctggtg ctggacgacc
     3061 cggatcaatc ggcgcgggca cgcgatctgt tcgccgccga gcaggtcttc atccccgaca
     3121 cggtcgttct ggaaacggcc tgggtgctgg cctatagcta cgaactgggc aaggatgcta
     3181 tcgccgtcgg actgcggcga ctgttcggac tgccgaacgt gcatctgcgg gacgcggccg
     3241 agatggcatt ggcgctggaa tggtacggcc gcggcctgga tttcgccgac gcgctgcacc
     3301 tggtgcaatc ggcccactgc tcccggctgc tgacgttcga ccgccgcttc gttgcccgcg
     3361 gcgcggggat tagtggggtg gctgtggcgt tggtgggttg acgtagaaca aactgtccag
     3421 cttgtttctt cccccagaca ggaatgcctg ctctacagtt attaaccatt tcacccaatt
     3481 tgcgatagca ttaccctcat acaccgcagg caaggagata cccctcatgg cacttaccca
     3541 cgtccccacc cgcagcgaag tcgatccccg tcacacctgg aacgccgaaa gcgtttttgc
     3601 taccgtcgat gagtgggcgg ccgaattggc ggccctgtcg gccggattgg gcgagcttga
     3661 ccgcttcaag ggccatctgg gcgacggccc cgacgtgctg gccgacgcgc tggagagcac
     3721 gcaagcctat ggtcaacgtg tcgagatgct gctgatctat accgcgctga ctgagtcggt
     3781 cgaccgcagc gatcaggcgg ctaccgttcg cgccgggcag gcgggcagcc tctatgggca
     3841 ggtggcgggg gccacggcct acatcgagcc ggagctgctg gccattggtc aggaccggtt
     3901 gaccgaatgg gtcgccaccg agccgcgcct gagccaatat gcccactact tcgacaccct
     3961 gtttcgtcgc caggctcacg tgcgctcgtc ggaggtggaa gaggtgattg ggctggcggc
     4021 cgatgcgttc ggcggcacgc gctccacttt ctccaaactg accgacagtg atttcaagtt
     4081 ccgccccgtc actttgcccg gacaggacga tcttcccgtg acacaaggga cggccggtga
     4141 tttgatccag tcgcccgatc gcgacgtgcg gcgcatcgcc tgggagaact atcacgacca
     4201 gtacctggcc tacaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctg
     4321 cctggatgag cgctgggtcg acatctaccc caacgagggc aagtcgtcgg gcgcgttctc
     4381 gtggggatgc cagggcacct atccgttcat tgtcatgagc tacaccgatg acgccaacag
     4441 catgggcacg ctggcccacg agttgggcca ctcgatgcac tcgttcctgt cctgggagaa
     4501 ccagcctcac atctattcca actactccct gttcgcggcc gaggtggcgt cgaatttcaa
     4561 tcaggccatg ctgcgtgcct atttgctgga gcaggacatc gaccgccaac tcaaaatcgc
     4621 cgtgctggaa gaggcaatga gcaatttcca tcgctacttc ctggagatgc ctacgctggc
     4681 cttgtttgag ctggagatgc accggcggga ggaacgcggc cagggactga cggccgacga
     4741 catgatcgca tacatggccg acttgttcgc cgagacctat ggcgacgcgg ccgccgtgga
     4801 cccgcaacgg gacggcatcc gctgggcgac attcggccat ctctacgccg attactacgt
     4861 ctaccagtac gccaccggca tctccggggc caacgcgctg gcccggcgcg tgttgtcggg
     4921 gcaatcgggc gcggctgcgg attacctcgg cttcctcagc gccgggggtt cgcgctaccc
     4981 gctggaggcg ctgcgctcgg ccggggtcga tctgacgcag cccgcgccgg tcgaggcggc
     5041 gtttgaggtg ctgggcgggt tgattgacaa gctggatgct cttgtgaatt agtagatgct
     5101 cggcggggta aatgatcacg cccggcgatt atcagatata tcaaccccgt ccggcgcgtc
     5161 cgtccatgag gcgatcccct gcgcgcatcg tcgttgtcgg cacaaccggc tccggcaaga
     5221 gcaccctggc cgagcggctg gcggcgcgca ttggcggcga ttttgttgat ctcgacgcgc
     5281 tgaactgggg gccggactgg acgcccgcgc ccgtcccgga atttcgcgag cgggccacgg
     5341 ccgcgcttgc cgctgaatgc tgggttgtgg ccggcaacta cagcaagctg cgcgacatcg
     5401 tctggggcgc ggccgacacg ctcatctggc tcgactaccc cctgccgctt gtcctctggc
     5461 gcctgctgcg ccgcacgatc cgccgtgttg ccacccgcga ggagttatgg aacggcaatc
     5521 gcgagcgttt tcgggagcaa ttcgccagtc gtgagtcttt gtttctctgg gccatcaaga
     5581 cctacagccg acgacgccat gagtatccgg ccgcgctggc gctgccggag tacgctcatt
     5641 tgcgcgtgct gcgctttcgt tggccgcggc agatggagcg gtggttcaat tcgatgagta
     5701 atcgttgaag ggactgtaat ggcttgtatg tgctataatg ggtttact
//
LOCUS       DAOMQG010000211         5748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5908_length_5748_cov_8.821887, whole genome shotgun sequence.
ACCESSION   DAOMQG010000211 DAOMQG010000000
VERSION     DAOMQG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5908_length_5748_cov_8.821887"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PLC06_11270"
     CDS             <1..1207
                     /locus_tag="PLC06_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRO91068.1"
                     /translation="YGVLGALNEVPLNDGYVLDIGGGSMQISRVLGRRFQQGTSLPLG
                     ALALSERFIRKDPPAAAELDAIQREIATYLDTVGWLEERKKKDGPVELVALGGTDRNM
                     AGMAAARSAYPLNTLHGFVLTRDDLDENIRLLSTLPLSRRETLPGLHSDRADIILPGA
                     LVLRAVMERLQTGQVTISVNGLREGLFFEHFWKHLSYPVLSDVRQFSVLNKARVYQYS
                     KAHANQVRFLAGRLFSQLIPLHGFGREERELLEAAALLHDLGTLISYDDHHKHSQALI
                     INSGLPGFQPRETALIGLLSRYHRKGRPRIDGFESLLRDGDEELLMQLSAILRLAEFL
                     ERGRNANVDDISAHWDDETLYLTLFADEFPAVEIWEAERNAVELMSKAFGRRVLVESS
                     AAPGDWRTG"
     gene            complement(1234..2268)
                     /locus_tag="PLC06_11275"
     CDS             complement(1234..2268)
                     /locus_tag="PLC06_11275"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRO91069.1"
                     /translation="MRTYRLALIGFGNVGQGLATILRDQGQVIASQHGAEYRIVAVNT
                     GRHGSIYNPEGFDPGMLLAAYEQTGRVDTIPAPQYGWDVPRVIYESNADVIVELTSSD
                     LLSGEPAAGYIRAALRQDRHVVTTNKGPVALFYPELSAAAAERGVMFGVEGTVMGGTP
                     LLRVGRELLAAAGVSRIQGILNGTTNYILTRMADGESYKSALAEAQEKGYAEANPIAD
                     VDGYDAAVKLAILANLLLGVPLTMANVEREGIRELSTKDVVRAQKVGEVWKMVGWVER
                     TPEMVKACVRPIRLPQSHPLASVSGATNAVTFVTKLLGDVTIIGPGAGRVETGYAVLS
                     DLLEIHQRMM"
     gene            2367..4169
                     /locus_tag="PLC06_11280"
     CDS             2367..4169
                     /locus_tag="PLC06_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91070.1"
                     /translation="MKIFTNNRRRFGVGLWIAALLGVLLAMTPVTGPAVAQDDPPPTP
                     EPATQPVTQPVTQPAAETTTEPTVTATPPPPGDIRFGVIESFEDPAAAGRIGAAWTRA
                     RFQWAEVQPDSPRDWRPPLDDEELAAELDAGREVVGLLIGIPDWARDRRGLPRGLSLP
                     PDDPDNTWAAFVTETVSRYEGRIDRWIIWNEPDIADRNAPGHTWDGTLEEFFQLQRVA
                     YLAAKAANPDAVVHLGAFTYFWDPGYFSRFLDVVIADPEAAANNYYFDVATAHLYFQP
                     NTVYNVLYAFRYLLDAHGLDQPIWLVETNAPPMDDPYWTVDNWTLAVSLNEQAAFIPQ
                     AIAAAFAGGAERVSVYKLKDTEGDRAANPEPFGLVRWDDSRRPAFDTYRVAIRLLGKV
                     TAAERERWDEVAQVRLEQPGQTTTVLFARLPGGQEATVIATAKTAELVDMWGKRETIE
                     AENGVFTVELPGALCRQTIADYCMIGGTTYYLIQESAGSGRPADSKPAIGDEGTFVAA
                     LMPSPTPSTTPSPAPSTTSRPSSTPSPSQATTIAATEAPPTATPNPPPATSGQEAAPG
                     AGAFPVGLLILGAGIVLGMGLSGWLIVRRGGGGK"
     gene            complement(4218..4544)
                     /locus_tag="PLC06_11285"
     CDS             complement(4218..4544)
                     /locus_tag="PLC06_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRO91071.1"
                     /translation="MIESIITEELIHRTNGDGPKGLNVIKVSARSRTAAVAGAIAGIM
                     REAGHAEVQAIGAGAVNQAVKAIVIAKSYLAEEDVHIACVPSFVDVDIDDQERTAIRF
                     VIEPQR"
     gene            4694..>5748
                     /locus_tag="PLC06_11290"
     CDS             4694..>5748
                     /locus_tag="PLC06_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRO91072.1"
                     /translation="MSNTEHSAPPAGDSVRAQVFRAAGLVASLALLSRVLGLVREIVV
                     RQYLGVTTLEATAFDIAGRFPEAIFLIVAGGAIGSAFIPTFTAYFSRDDEAGGWHLFS
                     AIINLVTIITTAVSVLVMIFAGPFVTFFYAENIALEPTLLPLTVYLMRIMLLSPIIFG
                     VSGIIMATLNARQHFLLPAVAPSVYNVGIIVGGVIGAWVGRGDPPATAMGLAWGTVAG
                     SLGHLLVQLPALRGRNARYAPVLSLHDPGVQQVARLMGPRVLGLSFSEINKFVILFLT
                     GTMALGTLPALNAAFRILIMPQGILGQALGIAAFPTLATLAARSAHREMRHILSDSLR
                     LILFLGLPATVLLIMLA"
BASE COUNT         1012 a         1887 c         1792 g         1057 t
ORIGIN      
        1 ttacggcgtg ctgggcgcgc tgaacgaagt cccgctgaat gacggctacg tgctcgacat
       61 cggcggcggc agcatgcaga tttcccgcgt actgggccgc cgcttccagc aagggacgtc
      121 tctgcccctt ggcgcgcttg ccctctccga gcgtttcatt cgcaaagacc caccggcggc
      181 ggccgaactc gatgcaatcc agcgcgagat cgcgacgtat ttggacaccg tgggttggct
      241 ggaagaacgc aagaagaaag acgggccggt ggaattggtg gcgctgggcg gcacggaccg
      301 caacatggcc gggatggccg ccgcccgctc ggcctacccg ctcaacacgc tccacggctt
      361 cgtcctgacg cgtgacgacc tggacgagaa catccgtctg ctgtcaacgc tgcccctgag
      421 ccggcgcgaa acgctcccag ggcttcacag cgaccgcgcc gacatcattt tgccgggcgc
      481 actcgtcctg cgggctgtga tggagcgact ccagaccggg caagtgacga tttcggtcaa
      541 cggcctgcgc gaggggttgt tcttcgagca tttctggaag cacctgagct accccgtcct
      601 cagcgacgtg cggcagttca gcgtgctcaa caaggcgcgg gtctatcaat acagcaaggc
      661 tcacgccaat caggtgcgct tcctggccgg gcggttgttc agccaattga ttccgctcca
      721 tggcttcggc cgcgaggaga gggagctgct cgaagccgcg gcgctgctcc acgatctggg
      781 cacgctgatc agctacgacg atcaccacaa gcattcacag gcgttgatca tcaacagcgg
      841 gttgccgggc tttcaaccgc gcgagacggc gctcatcggc ctgctgagcc gctatcatcg
      901 caagggacgg ccgcgcatcg acggcttcga gagtctgctg cgcgacggcg atgaagaact
      961 actcatgcaa ctgtcggcga tcctgcggct cgcggaattc ctggagcgcg gccgcaacgc
     1021 caacgtcgat gacatcagcg cccattggga cgacgagacg ctctatctga cgctcttcgc
     1081 cgacgaattc ccggcggtgg agatatggga agccgaacgc aacgcggtgg aactgatgag
     1141 caaggcgttt ggccggcgcg tgttggtgga gagttcggcc gctcccggcg actggcgaac
     1201 cggatagaag cgccttatcc gtggattccc cgcctacatc atccgttggt ggatttcgag
     1261 cagatcggac aggacggcat agccggtctc gacgcggccc gcgccggggc cgatgatggt
     1321 gacatccccc agcagcttgg tcacgaaggt gacggcattg gtcgcgcccg ataccgacgc
     1381 cagcgggtgg ctctgcggca gacgaatcgg ccggacgcag gccttcacca tttccggcgt
     1441 gcgctccacc caaccgacca tcttccacac ctcgcccacc ttttgcgccc gcacgacatc
     1501 cttcgtcgac agttcgcgga tgccctcgcg ctccacgttg gccatcgtca gcggcacgcc
     1561 cagcagcagg ttcgccagga tggcgagttt gacggccgcg tcgtaaccgt ccacgtcggc
     1621 gatggggttg gcttcggcgt aacccttttc ctgggcctcg gccagcgctg atttgtacga
     1681 ttcaccgtcg gccatgcggg tcaggatgta gttggtcgtg ccgttgagga tcccctggat
     1741 gcgcgagacg cccgcggcgg ccaacagttc gcggccgacg cgcagcagcg gcgtgccgcc
     1801 catgacggta ccctcgacgc cgaacatgac cccccgctcg gcggcggcgg ccgaaagctc
     1861 aggatagaac agcgccaccg gccccttgtt ggtcgtcacg acatgacgat cctggcgcag
     1921 cgcggcgcga atatagcctg ccgccggctc cccgctcagc agatcgctgg aagtcaactc
     1981 aacgatgacg tcggcgttgc tctcatagat gacgcgaggc acgtcccagc cgtattgggg
     2041 cgcggggatc gtgtccacgc ggccggtctg ctcataggcc gccagcaaca tgccggggtc
     2101 aaatccttcg gggttgtaga tgctgccgtg gcggccggtg ttgacggcca caatgcgata
     2161 ttccgcgccg tgctggctcg cgatgacctg gccctgatcg cgcaggatcg tcgccaaacc
     2221 ctggccgaca ttgccgaaac cgatgagcgc gagtcgataa gtgcgcatga tgggatgatt
     2281 acctgtcctt ccttaagaat atcggccgat tgtatcacac aattgcttgc cgtcccgccc
     2341 cctgtcgcca tacactatgg ggctggatga aaatttttac gaacaatcgt cgccggttcg
     2401 gcgttggctt gtggatagcg gcgctgctgg gggtgctact ggcgatgacg ccggtgaccg
     2461 gcccggccgt cgcccaggac gatcccccgc ccaccccgga gccggcaaca cagccggtga
     2521 cacagccggt gacacagccg gcggccgaaa ccaccacaga gccgacggtg acggctaccc
     2581 cacccccgcc cggcgatatt cgcttcggcg tcatcgaaag cttcgaggat ccggccgccg
     2641 ccggtcggat cggcgcggcg tggacgcgcg cccgtttcca gtgggccgag gtgcaaccgg
     2701 atagtccccg cgattggcgg ccgccgcttg acgacgagga gctggcggcc gaactggatg
     2761 ccgggcgcga ggtcgtcgga ttgctcatcg gcatccccga ttgggcgcgc gaccggcgcg
     2821 gattgccgcg cgggctgtcc ctgccacccg acgatcccga taacacctgg gccgccttcg
     2881 tcaccgaaac cgtgtcgcgc tatgagggcc gcatcgaccg ctggatcatc tggaacgagc
     2941 cggacatcgc cgaccgcaac gcgcccggtc acacctggga cggaacattg gaagagttct
     3001 tccagttgca gcgcgtagcc tatttagcgg ccaaggcggc caacccggac gcggtcgttc
     3061 atctgggcgc gttcacctac ttctgggatc cgggctattt ctcgcgcttt ttggatgtgg
     3121 tgatcgccga tcccgaagcc gcggccaaca attattactt tgacgtcgcc acggcccacc
     3181 tctactttca gcccaatacc gtctataacg ttctctacgc cttccgctac ctcctggacg
     3241 cccatggact ggatcaacca atctggctgg tggagaccaa cgcgccgccg atggacgacc
     3301 cctattggac ggtcgacaac tggacgttgg ccgtgtcgct gaacgaacag gcggctttca
     3361 tcccgcaggc catcgccgcc gcgttcgccg gcggggcgga gcgcgtctcg gtctacaagc
     3421 tgaaggacac agagggggat cgggcggcca acccggagcc gttcggcctg gtgcgttggg
     3481 acgacagccg acgccccgca ttcgacacct accgtgtagc tattcggctg ctgggcaagg
     3541 tcacagccgc cgagcgcgag cggtgggacg aggtggccca ggtgcggctg gagcagcccg
     3601 ggcagacgac gaccgtgttg ttcgcccggc tgccgggggg acaagaagcg accgtcatcg
     3661 ccaccgccaa aacggccgaa ctggtcgaca tgtggggcaa gcgcgagacc atcgaagcgg
     3721 aaaacggcgt cttcaccgtt gaattgccgg gagcgctctg ccggcaaact atcgccgatt
     3781 actgcatgat cggcgggacg acgtattatt tgattcagga aagcgcgggc agcgggcgac
     3841 ccgccgacag caagccggca attggggatg agggcacctt tgtggccgcg ctcatgcctt
     3901 cgccgacgcc gtcgaccacg ccatcccccg cgccatcgac cacgtcgcgg ccgtcctcca
     3961 ccccctcgcc cagccaggcc accaccatag ccgcaaccga agcgccgcca accgccacgc
     4021 cgaacccacc accggcaaca tcgggacagg aagccgcccc cggcgcgggg gctttcccgg
     4081 tgggtctgct catcctggga gcggggattg ttctgggtat gggcctatcg ggatggctga
     4141 tagtccggcg cggaggtggc gggaaataaa aagggcgcag ccgcgaggtt gcgcccgtgc
     4201 aggaaacgga acaacgacta acgttgcggc tcgatgacga accgaatggc ggtgcgctcc
     4261 tgatcgtcaa tgtcgacatc gacaaaagaa ggaacacatg caatatgaac atcttcttcg
     4321 gccagataac tcttggcgat cacgatggct ttcacggcct gattgacagc gccggcgcca
     4381 atggcctgga cctcagcatg gcccgcttcg cgcatgatcc cggcaatcgc gccggccacg
     4441 gccgccgtcc gcgagcgcgc ggacacctta ataacattca ggcccttcgg cccatcgcca
     4501 ttagtacgat gaatcagttc ctcggttatg atgctttcta tcatggttga ctattctcca
     4561 atgttcagta caaacgaccg ttatcagaac catagaataa cattgaagcc atgtcatttg
     4621 ttaggtcttt aatgcccaat tacgaggata gtagaatgtc ggagaacatc cccccgccaa
     4681 gggccgatca gccatgagca acacggaaca ttctgccccc ccggccggcg actcggtgcg
     4741 cgcgcaagtc tttcgcgccg cggggctggt ggcttctctg gctctcctca gccgcgtgct
     4801 gggcctggtg cgcgagatcg tcgtccggca gtatctgggc gtcacgacgc tggaggccac
     4861 cgcattcgac atcgccggtc ggttcccgga ggctatcttc ctgatcgtcg ccggtggggc
     4921 tattggctcg gcgttcatac ccacattcac cgcttacttc agccgcgatg acgaagcggg
     4981 tggctggcat ctcttttcgg ccatcatcaa tctggtcacc atcatcacaa cggccgtctc
     5041 ggtgctggtg atgatcttcg ccgggccgtt cgtcaccttt ttctacgccg aaaatatcgc
     5101 tctggagccg acgctgctcc cactcacggt ctatctgatg cggatcatgc tgttgtcgcc
     5161 gatcatcttc ggcgtcagcg gcatcatcat ggccacgctc aacgcccgcc agcatttcct
     5221 gctgcccgcg gtcgcgccct cggtctacaa cgtgggcatc atcgtcggcg gcgtcatcgg
     5281 cgcgtgggtc gggcgcggcg acccgccggc cacggccatg gggttggcct ggggcacggt
     5341 ggccggttcg ctgggccatc tgctggtgca gctgccggcg ctgcgcggtc gtaatgcccg
     5401 ctacgcacct gttctgtcac tgcatgaccc cggtgttcaa caggtggcgc gtctgatggg
     5461 accgcgcgtg ttggggcttt ccttcagcga aatcaacaag tttgtcattc tgtttctgac
     5521 cgggacgatg gcgctgggaa cattacccgc gctcaacgcc gccttccgca tcctcatcat
     5581 gccccaaggg attctggggc aggctctggg gatcgccgcc ttcccgacgc tggcgacgct
     5641 ggccgcccgt tcggcccacc gtgagatgcg tcatatcctc agcgattcgc tgcggctcat
     5701 cctctttttg gggctgccgg ccacggtgct gctcatcatg ctggccga
//
LOCUS       DAOMQG010000212         5740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5923_length_5740_cov_11.674230, whole genome shotgun sequence.
ACCESSION   DAOMQG010000212 DAOMQG010000000
VERSION     DAOMQG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_5740_cov_11.674230"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1333)
                     /locus_tag="PLC06_11295"
     CDS             complement(2..1333)
                     /locus_tag="PLC06_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91073.1"
                     /translation="MLAYRSRIPLALGTILLGIFLSLVAIHPARGAAEAVATEAVATE
                     AVGVIAYVRGNEIRQVNTDGTGDKRLWYEPLPNSAYKGIRGLQWRPDGGALAFASDFQ
                     QLCSLYESDIYSINAAGTGFRRLTNSPGCAQLAGFPKGTVRVQVENQTTESQFLVYVE
                     GAPIAKLTTIAPGTAVELTIPNVADLGNFQQEITVINGHKRWIDPSVVVNVTAGQTAN
                     ATGRFVLYGSGNVYSGVGAKYPTWHRSGSKVGFLYYEGLMLQIGANPPVAGPDSFILS
                     QSAGVIADGMAWSPASDWILYSSSDHISVVQPGATNGGEDIIDKAATELVLGLDWMPD
                     ESGFVFAITGGQFSPENSNIYIYDFGDNSLTPLTNFSDDFAGGLTISPNGQKIVFEFA
                     DEPGDPPQLWIMDINGKNRKPLGIQGEMPDWKPGTGINYNHRTLLPMVIRK"
     gene            1553..2698
                     /locus_tag="PLC06_11300"
     CDS             1553..2698
                     /locus_tag="PLC06_11300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRO91074.1"
                     /translation="MTFHTLPVLDAHIHYDGPASMLNLIEILDRCGIGKFNVVCTPHT
                     NRLSLVPDALHLKAHYPERVYVFGGLDISAYFILPDSCGAYFADYLDTLVRMGCDGVK
                     MIEGKPGMRKSLSVPPFDGLAYAPYWAKMEALGLPLVFHVNDPEEFWDPTLIPDWAVQ
                     QGWFYGDGSFVSNEVQYDEILNVMRRHPGLKMILAHFFFLSNQLPRLAGYLDEFPNMH
                     VDLTPGVEMYYAFARNPAATRDFFLKYQDRIIFGTDIGAKALLSGSGGIDLAESQIRV
                     DLIRTFLEADGEFRLVSDQGFLMDKFAGPFHGICLPMDVLEKIYHRNFERLAGERPRP
                     LDPDAIIRECDRLATIIPVMGAARPGEQADPSVAEMISLYFRGQKGA"
     gene            2704..4065
                     /locus_tag="PLC06_11305"
     CDS             2704..4065
                     /locus_tag="PLC06_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO91075.1"
                     /translation="MRTIKKWQEPIYAIGGFGPGFMYQLVLTYLLYFYRPAQARIETG
                     ALVFAPAGAYALGMLLARVLDGVLDIPIAGWTDNMKSRWGRRRPLMVLGIIPAIICFV
                     LLWFPPFTGVNLGPDGHWANAVYIAVVSSLYFFFHTLIIVPYLAALSEIVPDEQSRVR
                     VASWQTLFNTGSFILVFVVAPILFNRFGVRGAALMLLPSFITFIGPLLVIKEEPSNTA
                     EAREASKDVPLWESVRLTLSNRTFLIYMSTVATFFFGLQLFLGGIAFMAADMMGISDA
                     RLGLMNAAAFAPIPVMLILFNLISRKRGAKFAFRVALLIFAVAMLLFPLGWTRLNLPV
                     PPLVVGIIAGIIASFSIGAFFTIPYAFPAQIAAIEAAQTGKDRAGMYFAVQGLINQFV
                     GSLAGAFLALLLTWRLGVVVIGPLVAVFMVIAFVLFAPYPLGQPTEHTELKVPEGGQG
                     EMA"
     gene            4244..4537
                     /locus_tag="PLC06_11310"
     CDS             4244..4537
                     /locus_tag="PLC06_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRO91076.1"
                     /translation="MVDDTRSYSAQVRQRGQLTIPRKLRESLRIEDGDTLTFMPMGET
                     LVLVPRRLRAPELIDRLATMLEDSDQSLADLLIDLPRIREALYNERYAAGQGE"
     gene            4542..4982
                     /locus_tag="PLC06_11315"
     CDS             4542..4982
                     /locus_tag="PLC06_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRO91077.1"
                     /translation="MRRVFFDANVIIAGSVSRRGASRALMMLAEMGMFRMVVSRQVID
                     EVERNLRNKLPQGLPVMVELLGYIAPEIVEDPPAEALARWRGHIETKDIPVLEAAVSA
                     DADFIVTLNSRDFTPKIATLSGVPIVSPGELIERIRASVTEGLE"
     gene            complement(5030..>5740)
                     /locus_tag="PLC06_11320"
     CDS             complement(5030..>5740)
                     /locus_tag="PLC06_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRO91078.1"
                     /translation="SCNSCFYDVGYTIDGTDNTLLPRIAREFGLGQPTGIQGIAESAG
                     LIPDPEWKLANIGEGWATGDAVNMSIGQGYVQVTPLQMANIAAAIANGGTLYRPTLVD
                     RIGESPTLPEEMPESHVNGVLPLSAEQLAVVRESLWNVTHAGNGTATHRFVDMPVPVA
                     GKTGTAEAPPGLPHAWFVGYAPAEPYTTIDGRLVETPELAIAVVLEHAGEGSDVAAPL
                     FRRVVELYYGIEPAPFPW"
BASE COUNT         1077 a         1688 c         1709 g         1266 t
ORIGIN      
        1 gtcacttcct gattaccatc ggcaataatg tccgatgatt atagttgatg cccgtgccgg
       61 gtttccagtc aggcatttcg ccctgaatgc ccagcggctt tcggtttttg ccgttaatat
      121 ccatgatcca caactgcggt gggtcgccgg gttcgtcggc gaactcgaag acgatcttct
      181 gtccattggg cgagatggtg agtccaccgg cgaaatcgtc ggagaaattg gtcagcggtg
      241 tcaggctgtt gtccccgaag tcgtagatat agatattgct gttttccggg ctaaactgcc
      301 cgccagtgat ggcgaagacg aagccggatt cgtcgggcat ccagtccagc cccagcacca
      361 actcggtggc ggccttgtcg atgatatcct ccccgccgtt ggttgcgccc ggctgcacaa
      421 cgctgatatg gtcgctggac gaatagagga tccagtcgct ggccggcgac caggccatgc
      481 cgtcggcaat gacgcccgcg ctctgggaaa gtatgaacga gtcggggcct gcaacagggg
      541 gattggcgcc gatctgcaac atcagcccct cgtagtacag gaatccgacc ttggagccgc
      601 tgcgatgcca ggttggatac ttcgcgccga ctccggaata gacattgccc gagccataaa
      661 gcacaaagcg acccgtggca ttggccgtct gtccggccgt gacattgacg acgaccgacg
      721 gatcgatcca gcgtttatgc ccattgataa ccgtgatctc ctgctggaag ttgcccagat
      781 cggcgacatt gggaatggtg agctccacgg ccgtcccggg ggcaatcgta gtcagcttgg
      841 ctatcggcgc tccctcaacg tagaccagga attgcgattc cgtggtctga ttttcaactt
      901 gcaccctgac cgttcccttg ggaaagccgg ccaattgggc gcagccgggg gagttggtca
      961 gccgcctgaa tccggtaccc gccgcgttga tgctgtagat atcgctttca tagagggagc
     1021 agagctgctg gaaatcggat gcaaaggcca gcgcgccgcc gtccggccgc cattgcaggc
     1081 cgcggatgcc cttgtaggct gaattgggca gcggttcata ccacaagcgc ttgtcgccgg
     1141 tgccgtcggt gttgacctgt cggatctcat tgccacggac gtaggcgatg acgccgaccg
     1201 cttcggttgc gaccgcttcg gttgcgaccg cttcggccgc gccgcgagca ggatgaattg
     1261 cgacaaggct gaggaagatg cccagcaaga ttgtgccgag cgcgaggggg atgcgggacc
     1321 tgtaagccaa catgggaagt accctcctgc ttcaaggatg tccggcaaat cgcctgggca
     1381 gaaagtacca taattcacgt catttgtcac gccacaacac gctattgtgc aactcttttc
     1441 tgcgcgtaaa ttggctaccg aaaactctcg tgcatggcgt aattgagatt gacgaagggg
     1501 tgagattggt tattattaca aactcataaa ttgttaatct ttggagccga ccttgacatt
     1561 tcatacgctt cccgttcttg atgcgcacat ccattacgat ggccccgcgt cgatgttgaa
     1621 cctgatcgaa attctcgacc gctgcggaat cgggaagttc aacgtcgtgt gcacgcccca
     1681 cacgaaccgg cttagtctgg tgccggacgc gctgcacctg aaggcccatt atcctgagcg
     1741 agtctatgtg ttcggtggtc tggatatttc cgcgtatttt attctccccg actcctgtgg
     1801 cgcgtacttt gccgactacc tcgacactct ggtgcgcatg ggctgcgacg gggtgaagat
     1861 gatcgaaggt aagccgggca tgcgcaagtc gttatccgtg ccgcctttcg acggcctggc
     1921 ctatgctcca tattgggcga agatggaagc gctgggcttg ccgctggttt ttcatgtcaa
     1981 tgatcccgaa gagttttggg atcccaccct catacccgac tgggctgtgc agcagggctg
     2041 gttttatggc gacggcagct tcgtttccaa cgaagtccaa tatgacgaaa tactgaacgt
     2101 gatgcggcgc catcccggcc tgaagatgat ccttgcccat ttcttctttc tctccaatca
     2161 gctgccgcgt ctggccggct acctggatga attccccaac atgcacgttg acctgacccc
     2221 gggcgtcgag atgtactatg ccttcgcccg caacccggcg gcgacgcgcg atttcttcct
     2281 gaaataccag gatcgcatca tttttggcac cgatattggc gcgaaggcgt tgttatccgg
     2341 ctccggcggc atcgatctgg ccgagagcca aatccgcgtc gatctgatac gcaccttcct
     2401 ggaggcggat ggggaattcc ggctggtctc cgatcagggc ttcctgatgg ataaatttgc
     2461 cgggccgttc cacggcatct gcctgccaat ggacgtgctg gaaaagatct atcatcgaaa
     2521 tttcgagcgt ctggcggggg agcggcctcg gccgctcgac cccgacgcga tcatccggga
     2581 atgtgatcgc ctggcaacta tcatcccggt catgggggcg gctcgcccgg gcgaacaggc
     2641 agacccatct gtggccgaaa tgatctctct ttatttccgt gggcaaaaag gagcttgacg
     2701 aacatgagaa ccatcaagaa gtggcaggaa cccatctatg cgattggcgg cttcgggccg
     2761 ggatttatgt accagctggt cctgacctat ctgctctatt tttaccgacc cgctcaggcg
     2821 cgaatagaga ccggcgcgct ggtgttcgcc cccgccggcg catacgctct tggcatgtta
     2881 ctggcccgcg tgctggatgg cgtattggat atccccatcg ccgggtggac ggataatatg
     2941 aagagccgct ggggtcgccg ccggccgttg atggtgctgg ggatcatccc cgcgattatc
     3001 tgcttcgtcc tgttgtggtt tccgcccttc acgggggtca atctggggcc ggacggtcac
     3061 tgggccaacg cggtctatat cgccgtcgtc agttcgctct atttcttctt ccacacgctc
     3121 atcatcgtcc cctatctcgc cgctctctcg gaaattgtgc ccgatgagca gtcgcgcgtt
     3181 cgcgtcgcca gctggcagac gttgttcaac accggcagct ttatcctggt atttgtggtt
     3241 gcacccattc tgttcaaccg ttttggcgtg cgcggcgcgg cccttatgct cctgcccagc
     3301 ttcatcacgt tcatcggccc cttgctggtc atcaaggaag agccatccaa cacggccgag
     3361 gcccgggagg cctcgaaaga tgttcctctc tgggagagcg ttcggctcac tctgtccaat
     3421 cgtacctttc tgatctacat gtcaacggtg gcaacctttt tcttcggttt gcagctattt
     3481 ctgggcggca tcgcctttat ggccgccgac atgatgggta tttccgacgc caggctgggt
     3541 ctgatgaacg cggccgcctt cgcgccgatc cccgtgatgc tgatcctttt caacctgatc
     3601 tccagaaagc ggggcgccaa gttcgccttc cgggtggcat tgctgatttt tgccgtggcg
     3661 atgctgctct tcccactggg gtggacgaga ctcaatctgc ccgtgccgcc tttggtcgtg
     3721 gggattatcg caggcataat cgccagcttc tccatcggcg catttttcac cattccctac
     3781 gccttcccgg cccagattgc cgcgatagag gcggcgcaaa ccggcaagga tcgcgccggg
     3841 atgtacttcg ccgtgcaggg gttgatcaat cagtttgtcg gctcgctggc cggcgcattc
     3901 ctggcgttgc tgttgacctg gcgtctcggc gtggttgtca tcggcccgct ggtggctgtg
     3961 tttatggtga tcgcttttgt gctcttcgcg ccctacccat taggtcaacc gacggaacac
     4021 acggaattaa aggtgcctga aggggggcag ggagagatgg cctaggccgg acgaattacc
     4081 gctcgatcat tccatctgtc gattgcgggg atggccatgc ttatgccgaa gatggccatg
     4141 ttgatcgccg ctacctcgtt ctgtataatg ggattatgaa aatcggaaat ctcgtaattc
     4201 cgttatacag aaattcagaa aatatgaaag agggtaaaag gagatggtcg acgatactcg
     4261 atcctattcg gctcaggtca ggcagcgcgg tcaattgacg atcccgcgca agctgcgcga
     4321 atcactcaga attgaagacg gcgacacatt gacattcatg ccgatgggcg agacgttggt
     4381 gctcgtgccg cgccggctgc gtgccccgga actgatcgac cgtctggcaa cgatgctgga
     4441 agacagcgat caatcattgg ccgatttgct gatcgacctg ccccgcatcc gcgaagccct
     4501 gtacaacgaa agatacgcgg ccggccaagg ggagtaacgc catgcgtcgg gttttcttcg
     4561 acgccaacgt catcattgcc ggcagcgtgt cgcgaagagg cgcaagccgg gccttaatga
     4621 tgctggccga gatgggtatg ttccgcatgg ttgtctcgcg ccaagtcatt gacgaggtgg
     4681 agcgcaacct gcggaacaag ttgccgcagg ggctgccggt gatggtggaa ctgcttggct
     4741 atatcgcgcc ggagattgtg gaagatcccc cggccgaagc gttggcgcgc tggcgaggcc
     4801 acattgaaac caaggatata cccgttctgg aggccgccgt gtccgcggat gcagatttca
     4861 tcgtgacact caacagccgc gactttacgc caaagatagc aaccctctcg ggcgtcccca
     4921 tcgtatcccc cggcgaactc atcgagagaa tcagagcgag cgtgaccgag gggctggaat
     4981 agatggcccg gcccccggtt gaggctgcag gccgcggcgt gcgcccctgt taccacggaa
     5041 acggcgccgg ctcaatcccg taatataact ccaccacccg ccggaagagc ggcgcggcca
     5101 catccgaccc ctcgccggcg tgttccagca ccacggcaat ggccagctcc ggcgtctcca
     5161 ccagacggcc gtcaattgtc gtgtacggtt cagccggcgc atagccgacg aaccaggcgt
     5221 ggggcagccc cggcggcgct tcggccgtgc ccgtcttccc cgccaccggc acgggcatat
     5281 ccacaaagcg atgggtcgcc gtgccgttgc cggcgtgggt cacgttccac agactctcgc
     5341 ggacgacggc gagctgctcg gccgacagag gcagcacgcc gttgacgtgt gactccggca
     5401 tctcttcagg cagggtcggc gattcgccga tgcggtcgac cagcgtcggc cgatagagcg
     5461 tgccgccatt ggcaatggct gcggcgatgt tggccatttg cagcggcgtc acctgcacgt
     5521 agccctggcc gatggacatg ttcaccgcgt cgcccgtggc ccatccctcg ccaatgttgg
     5581 ccagtttcca ctccggatcg ggaatcagac ccgccgactc ggcgatgccc tggatgcccg
     5641 ttggttgacc caggccgaac tcacgggcaa tgcgcggcag cagggtattg tctgtgccgt
     5701 cgatggtgta gcccacgtcg tagaagcagg agttgcacga
//
LOCUS       DAOMQG010000213         5739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5924_length_5739_cov_10.054011, whole genome shotgun sequence.
ACCESSION   DAOMQG010000213 DAOMQG010000000
VERSION     DAOMQG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_5739_cov_10.054011"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLC06_11325"
     CDS             <1..285
                     /locus_tag="PLC06_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91079.1"
                     /translation="LALPLLVMLITMYIGKLPGRMKRLTWLLFLVYVLQADVLIFLRV
                     SAPILSAFHPVLALIDFALGLMLARQAGVIVQIGETPVAQSLQPDNAIGD"
     gene            316..1326
                     /locus_tag="PLC06_11330"
     CDS             316..1326
                     /locus_tag="PLC06_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91080.1"
                     /translation="MTNDDTDLSSRTFLAVANRRVVRAMCCKDGSWQVATVLDGPSPV
                     TLTADPQNPDVVYAGTQGNGVYRSADRGRNWDSLGLAGRIVKSLAVSPHDPDVIYAGV
                     KPALVYKTNDGGKNWVELEGFRHVPNRWWWFSPAEPPFRAYVNALAVSPAEPYVVLAG
                     IEFGALVRSEDGGRTWSRHRPGAMRDCHSLGFHHRDGRWVYEAGGTGGGAAVSRDGGR
                     TWKQPKSGLAKKYGVVCAADPARPEVWYVAVAPGPGKAYGQNPEAYLYRASAGADWEP
                     IGWEPQPMRQMPRALVTRPDAPGQLFVGTTYGDVWHTADYGDSWRRLPFDLGGGLSLL
                     VL"
     gene            1354..2256
                     /locus_tag="PLC06_11335"
     CDS             1354..2256
                     /locus_tag="PLC06_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRO91081.1"
                     /translation="MKKVLKRIGILLAGLVGVIVVIALVGYFVSERRINKTYAIAVEP
                     LTVPTDAADIEEGHRLTVIRGCVDCHGTDYGGTTMLEDPIIGRIYGANLTTGQGSATA
                     GWSAEDWSRAIRNGVAPDGRALLVMPSLEYSGLSDEDVGRIIAYLRSAPPVDRILPES
                     SLGSMTRAMLVTNQPPPLLSAEVIDPSNRPPKSLVPEASVEYGAYQVTTCTGCHGKNL
                     GGGPVPFSPPDKPKAANLTQGGDLANWTLEQFKTTLRTGITPEGRQLDPQNMPWPVTS
                     QMTDVELEALWLYFHSLPPVMAEN"
     gene            2338..2832
                     /locus_tag="PLC06_11340"
     CDS             2338..2832
                     /locus_tag="PLC06_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91082.1"
                     /translation="MYQWLVYIHVLATFAFLLTHGVSSVVALRLRNQRDPAVARAWLE
                     LNASGSVIAVLYGSLLTLLISGVINGFMGKWWGSGWIWLSLVLLIGIIVSMGLIGSRY
                     YSQVRKALGMAWFDGRKEHPAGEPASAEEIEALLAGGPAITLTVIGFGGIAVILWLMM
                     FKPF"
     gene            2892..5024
                     /locus_tag="PLC06_11345"
     CDS             2892..5024
                     /locus_tag="PLC06_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRO91083.1"
                     /translation="MRETERITSPLSLASATKADDATPRLMRLARALAAVVIVVMALN
                     FVMVVWYVVRTGDTTSFLSHQMMTPFLSIIYTGLGVVIIKHRPRNPIGWIFLIVSSSY
                     VLSALAAGALLYTSILPAPLGPLLLDLATWLGNWAWLPAQLLPLTFVFLLFPDGRLLS
                     PRWRIIGWAAGLGLLSLMLGLAAHPGPIADWASQPNPFGIAGAERALDSLMTVGSFLL
                     AIGVFGSMAAIAVRFRRSKGIERARMKWLVYAAILILIAGAFTLPYWLSGNLSNGLAL
                     ELSIVLTNLVTLGIAVAVTIAIVGYRLYDIDVIINRTLVYSVMTGVIILIYSLVVGTA
                     SVFFGSSGNWLLALVATGLVAVLFQPIRDRLQRGVNRLLYGQRDEPFEVLTRLGQSLE
                     QSAADTALPALVDTVAQALRLPYVDLQERQGDRFVTVAATGRPVDDPVSFPMIYQAEE
                     IGRLLVGSRTPGETLGAADEHLLRNIARQAGATVYDTRLTADLQRSRQQIVASREEER
                     RRLRRDLHDGLGPSLASLLLEARVLRRMIREDPAAAEGLADEIQADIRATIDDVRRVV
                     NELRPPALDDLGLVPALQVMAAKLGRSDGQGAPGLSVQVDAPADLPSLPAAVEVAAYR
                     IVQEGLANVLHHAQARQAVVRIRVNGDLRVEVWDDGAGFQTRRRGGVGLISMRERAAE
                     LGGRCEISSTPGAGTLITATLPINELQP"
     gene            5021..5665
                     /locus_tag="PLC06_11350"
     CDS             5021..5665
                     /locus_tag="PLC06_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91084.1"
                     /translation="MTPLRILIADDHPLFRKGMRTLLASMPETEVVGEATTGKETVDR
                     ALALQPDIVLMDLQMPEGGGLTAIRSLTSANPETRILVVTLFEDDESVFAALKAGARG
                     YVLKDADEDEMIRAIQAVARGEAIFSPAVASRLMDYFTAARTSPHAEAFPDLTERERE
                     ILAMIARGRSNYEIAEELSISLKTVRNHASNIFSKLQVADRTQAAIRAREAGLT"
BASE COUNT         1012 a         1691 c         1821 g         1215 t
ORIGIN      
        1 ttggccttgc cgttgctggt catgttgatc acgatgtaca tcggcaaact gcccggacgg
       61 atgaaacggc tgacgtggtt gctgttcctc gtctacgtcc tgcaggccga cgtgcttatt
      121 ttcttgcggg tgtccgcccc gatcttgtcg gctttccacc ccgtgctggc gttgatcgat
      181 ttcgctctgg ggctgatgct ggctcggcag gcgggagtga ttgtccagat cggggaaacg
      241 ccggtcgccc aatcgctgca accggacaat gccatcggtg attgaatgat tgaccgggat
      301 tgagaaggaa ccgccatgac caacgatgat accgatctca gttcacgcac tttcctggcc
      361 gtcgccaacc gccgagtcgt ccgcgccatg tgctgcaagg acggtagctg gcaggtagcg
      421 accgtattgg acgggccgtc gccggtgacg ttgaccgccg atccccaaaa tcccgacgtc
      481 gtttatgccg gaacgcaggg caacggcgtc tatcgctcgg ccgatcgcgg ccgtaactgg
      541 gactcgctgg ggttggccgg gcggatcgtc aagtcgctcg ctgtcagccc ccacgacccc
      601 gacgtgatct atgccggcgt gaaaccggcc ctcgtctaca agaccaatga cggcggcaaa
      661 aactgggtcg aactggaagg cttccggcac gttcccaatc gctggtggtg gttctcgccg
      721 gccgagccac ccttccgggc ctatgtcaac gccctggctg tttcacccgc cgagccgtac
      781 gtcgtcctgg cgggaatcga gttcggcgcg ctggtgcgca gcgaggacgg cggccgcacc
      841 tggtcgagac atcgtcccgg cgcgatgcgc gattgtcact ccctgggatt tcaccatcga
      901 gatgggcggt gggtctatga ggccggagga acaggtggcg gggcggccgt cagtcgcgat
      961 ggcggccgca cctggaaaca accgaaaagc ggcctggcca aaaagtatgg cgtggtctgt
     1021 gccgccgatc cggcgcgacc ggaggtctgg tacgtggccg tggctcccgg accgggcaag
     1081 gcttatggtc agaacccgga ggcatatctc taccgggcct cggccggggc tgactgggaa
     1141 cccatcggct gggaaccgca accgatgagg cagatgccgc gggcgctggt tacccggccg
     1201 gatgcgccgg gccaactctt cgtcggcacg acttatggcg acgtgtggca tacggccgac
     1261 tatggtgatt cctggcggcg cttgccgttc gatctcggcg gtggcctgtc attgctggtt
     1321 ctttagaacg attgaggaag gatgaggaac attatgaaaa aagtactcaa gcggatcgga
     1381 atcctgctgg ccggactggt gggggtcatt gtagtcattg ccctggtggg ttatttcgtc
     1441 agcgagcgcc ggatcaacaa aacctacgcg atcgcggttg agccgctgac cgtgccgact
     1501 gacgcggccg acattgagga gggccaccgg ctgaccgtta ttcgtggctg tgtggattgc
     1561 cacggcacag attacggcgg gacgaccatg ctcgaagacc ccatcattgg tcgtatctat
     1621 ggcgctaatc tgacgacggg tcagggcagt gccaccgccg ggtggtcggc cgaagattgg
     1681 tccagagcga tccgcaacgg cgttgccccg gacggccgtg ctctattggt gatgcccagc
     1741 ctcgaatatt cgggattgag tgacgaggat gtcggccgaa tcatcgccta cctgcgttcg
     1801 gctccgcctg tcgatcgcat cctgccggaa tccagcctgg ggtcgatgac tcgcgccatg
     1861 ctggtcacca atcagccgcc gccgctcctc tcggccgaag tgatcgaccc atccaaccgg
     1921 ccgcccaaga gtctggttcc ggaagcgtcg gtggagtatg gagcatatca ggttacgacg
     1981 tgcaccggct gccacggcaa gaacctgggc ggtggcccgg tgccattcag cccgccggac
     2041 aagccgaagg cggcgaacct gacccaaggc ggcgatttgg ctaactggac gttggagcag
     2101 ttcaaaacca cgttgcgcac cggcataacg ccggagggtc ggcagctgga tccacaaaac
     2161 atgccctggc cggtgacgtc gcaaatgacc gacgtggagt tagaggcatt gtggctgtac
     2221 ttccatagcc tgccgccggt gatggccgag aattaagacg gggtttacat gagacctcct
     2281 ttcgtcacag tcctgatctc atcaatggtt tttaattaca ccccaggaga ttgacgtatg
     2341 tatcaatggt tggtttatat tcacgttctg gcgacgttcg ccttcctgct gacccacggc
     2401 gtgagtagcg tcgttgccct gcggctgcgc aaccagcgcg atccggctgt ggcgcgggca
     2461 tggctggaac tcaatgccag tggtagcgtc attgccgtgc tctatggttc gttgctcacc
     2521 ctgctgatta gcggggtaat caacggcttc atggggaaat ggtggggcag cgggtggatc
     2581 tggctctcgc tggttttgct cattggcatc atagtgtcca tgggtctcat cggttctcga
     2641 tattacagcc aggtgaggaa ggcgttgggt atggcctggt tcgatggccg aaaagagcac
     2701 ccggccggcg aacccgcttc ggccgaggag atcgaggcgc tcctggccgg agggccggcc
     2761 atcaccctga ccgtgatcgg ctttggcggg atcgccgtca ttctttggct catgatgttt
     2821 aagccattct agcgcgatgg aacgacggcg cactcccggg ctgaaaagcc atgttataat
     2881 gctctcgaat catgcgcgaa actgaacgaa tcacgtctcc cctgtccctg gcaagcgcga
     2941 cgaaagcgga cgatgcgaca ccccgcttga tgcggttggc gcgcgcgttg gcggcggtcg
     3001 tcattgtcgt gatggcgctg aactttgtga tggtcgtctg gtatgtggtt cgcacaggtg
     3061 acacgacgag ctttttgtcg caccagatga tgacgccttt cctgtcgatc atctataccg
     3121 gccttggcgt tgtcatcatt aaacaccgac cccggaaccc tatcggctgg atctttctga
     3181 tcgtgagttc gtcctacgtc ctcagcgcgt tagctgccgg ggcgctgctt tatacgtcga
     3241 ttctccctgc tcccctcggc ccgcttctgc tcgacctggc gacctggctg ggcaattggg
     3301 catggctgcc ggctcaactt ttgccgttga cctttgtctt tctccttttt cccgatggtc
     3361 gtttgctttc accgcgctgg cgaataattg gctgggcggc cgggctgggc ctgttgtcat
     3421 taatgctggg tctggctgcc caccccggcc ccatagccga ttgggcatca caacccaacc
     3481 ccttcggtat tgccggtgcg gaacgtgccc tcgatagtct gatgacggtg ggcagctttt
     3541 tactggcaat cggcgttttc ggttcgatgg ccgccattgc agtgcgcttc cgacgttcga
     3601 agggaatcga gcgcgcccgg atgaaatggc tggtctatgc cgccatcctc atcctgattg
     3661 ccggcgcttt tactctgccg tactggctct cagggaattt atccaacggt ctggccctgg
     3721 aattgagcat cgtcctgacg aatttagtga ccctggggat cgccgtcgcg gtgacgatcg
     3781 ccatcgtagg ctaccggttg tacgacattg atgtcatcat taaccggacg ctggtgtaca
     3841 gtgtgatgac cggcgtcatt atcctgattt acagcctcgt cgtcggcacg gcgagcgtct
     3901 tcttcggttc aagcggcaac tggctcttgg cgctggtggc caccgggttg gtggccgtcc
     3961 tctttcaacc tatccgtgat cgcttgcagc gcggcgtcaa tcgccttctc tatggccaac
     4021 gcgacgagcc gtttgaagtg ctcacgcgtc tgggacagag cctggagcag tcggcggcgg
     4081 acacggccct ccctgcactg gtagacacgg ttgcccaggc tcttcgcctg ccttacgtcg
     4141 atcttcagga gcgccagggc gaccgatttg tcactgtcgc cgccaccggc cgcccggttg
     4201 atgatccggt ttcgtttccg atgatctacc aggcggaaga gatcggccgg ctgctggtcg
     4261 gttctcgcac tcccggtgag acgcttggcg cggccgatga acacttgttg cgcaacatcg
     4321 cccggcaggc aggcgcgacg gtctatgaca cccggctcac ggccgatttg caacgttccc
     4381 gacagcagat cgtggccagt cgggaagagg agcgccgacg cctgcgccgc gatcttcacg
     4441 atgggcttgg cccatccctc gcgtcgctgc tcctggaggc acgggtcttg cggcgcatga
     4501 tccgcgaaga tccggccgcg gccgaggggt tggccgacga gatacaggct gacatccggg
     4561 caacgatcga tgacgttcga cgcgtggtca atgagctgcg cccgccggcg ctggacgacc
     4621 tggggcttgt gccggcgctg caggtcatgg cggccaaact gggccggagc gatggtcagg
     4681 gtgcgcctgg cctaagcgtt caggtcgatg cgccggccga tctgccgtct ctgcctgcgg
     4741 cggtcgaggt cgccgcttat cggatcgttc aggaagggtt ggccaacgtc ctccatcatg
     4801 ctcaggcccg gcaggcggtc gttcgcattc gggtgaacgg cgatctgcgg gttgaggtat
     4861 gggacgacgg cgcgggcttc cagacccgac gcaggggcgg cgtcgggttg atctccatgc
     4921 gggaacgggc ggccgagttg ggcggccggt gcgaaatctc atcgacgccg ggggccggga
     4981 ccttaatcac ggcgaccttg ccgatcaacg agttacaacc atgacgccgc tgcgaattct
     5041 catcgccgat gaccatccgc tgtttcgcaa aggaatgcgc acattgctgg cttcaatgcc
     5101 tgaaaccgag gtagtgggcg aagcgacgac cggcaaagag accgtggatc gggcgctggc
     5161 ccttcagccc gatatcgtgc tgatggactt gcagatgccc gaaggtggcg gcctgacggc
     5221 catccggtcg ctcacgtccg cgaacccgga gacgcgaatt ctggtcgtta ccttgttcga
     5281 ggacgatgag tccgtattcg ccgctctgaa agccggggca cgtggctacg tgctgaagga
     5341 cgccgacgaa gacgagatga ttcgcgctat tcaggctgtg gctcgaggag aagctatctt
     5401 tagcccggct gtggcttcac gtctgatgga ctactttaca gcggcgcgga catctcccca
     5461 cgcggaagct ttccccgacc tcaccgagcg cgagcgggag atattggcga tgatcgcccg
     5521 cggccgatcc aattatgaga tcgccgagga actttccatc agtctgaaaa ccgttcgcaa
     5581 tcatgcctcg aacatcttca gcaaactaca ggttgccgac cgaacacagg cggcgattcg
     5641 ggcgcgagaa gccggtttga cctgaggact caattcaata gccttatcgt agcggacaac
     5701 caaacgatac gatatacatg gatatcagct tattcgtag
//
LOCUS       DAOMQG010000214         5705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5968_length_5705_cov_8.247611, whole genome shotgun sequence.
ACCESSION   DAOMQG010000214 DAOMQG010000000
VERSION     DAOMQG010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5968_length_5705_cov_8.247611"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /gene="clpB"
                     /locus_tag="PLC06_11355"
     CDS             <1..2298
                     /gene="clpB"
                     /locus_tag="PLC06_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRO91085.1"
                     /translation="DDYTSTEHLLMAMAQPKGGRVRELLARHGIDYNAILQALAAVRG
                     SQRVTSQNPEAQYEALAKYGRDLTQEARRGKLDPVIGRDEEIRRVIQILSRRTKNNPV
                     LIGEPGVGKTAIVEGLAQRIVNGDVPLGLKDKRLVSLDLGALVAGAKYRGEFEERLKA
                     VLKEVQEAEGQIILFIDEMHTLIGAGAAEGAMDASNMLKPLLARGELHAIGATTLDEY
                     RKHIEKDAALERRFQPVFVDEPSVEDTISILRGLKERYEVHHGVRVTDAAVIAAATLS
                     HRYITDRFLPDKAIDLIDEAASRLRMQIDSKPAELDEIDRVIMQLEIEREALKKEKDK
                     ASQERLGRLEEELANAKERSIQLTGRWQAEKDAIQHIQTVKEEMDAVRIQVEQAQRDY
                     DYQKASELQFGRMRQLEHDLSEALAQLHQIQADGAMLKEEVDAEDIAYIVSKWTGIPA
                     TKLLEGEVEKLIYMEDRLHRRVVGQEEAVAAVAAAVRRSRSGLQDPNRPIGSFIFLGP
                     TGVGKTELARALAEFLFDDERSMVRIDMSEYQERHTVARLIGAPPGYVGYEEGGQLTE
                     AVRRRPYSVILFDEIEKAHPEVFNVLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNVGS
                     QFILGDLDEETMRARVMEALRAQFRPEFLNRVDEIVIFHRLGREQLREIAEIQLNNVR
                     GLLAKRDITLALTETATDLLIEEGYDPVYGARPIKRVLQRRIIDPLAIRVIQGDVRDG
                     DHVVVEVIDGQLDFRVVESGEFETA"
     gene            2401..2676
                     /locus_tag="PLC06_11360"
     CDS             2401..2676
                     /locus_tag="PLC06_11360"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRO91086.1"
                     /translation="MSIYSIRLYLEPNPDGQFTVTSPDVPGLVTEGDTPETIQANVQE
                     ALDALLDEDYFRQVSIDSAGGIVWPNDYDFCPNFLYEDVPAVESVAS"
     gene            2770..3429
                     /locus_tag="PLC06_11365"
     CDS             2770..3429
                     /locus_tag="PLC06_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO91087.1"
                     /translation="MIPPLQTGDHLTRAEFERRYSAQPHLKRAELIEGVVYVPSPVHL
                     DSHARPHSRIMGLLFNYMAATPGTDTADNASVRLDAENEVQPDAMLRIREEYGGQSRQ
                     SEDDYLIGAPELIVEIAASSAAYDLHEKLRVYRRTGVKEYVVLLTLELETVWYRLNEG
                     RYDVVSPDEDGVYRSAVFPGLHFHSANFWADDVAGQLAVLQAGIATPAHADFVAALRP
                     V"
     gene            complement(3436..3942)
                     /locus_tag="PLC06_11370"
     CDS             complement(3436..3942)
                     /locus_tag="PLC06_11370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRO91088.1"
                     /translation="MVIRAETPNDVAAIRLVNRPAFGREDEGALVDALRESRAVICSL
                     VAEDAGQVVGHVLYSPATLDDDGRLTSVAALGPVAVLPDRQRRGIGDALIRAGLDICR
                     EQGYGLAIVLGHSTYYPRFGFRPSRPLGIRWEYDAPEEAFMVMELRPGALAGARGVVR
                     YRPEFDGV"
     gene            4270..4707
                     /locus_tag="PLC06_11375"
     CDS             4270..4707
                     /locus_tag="PLC06_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO91089.1"
                     /translation="MKKDLVQDWMSTDVITIAPDTPLPEAHNIMTTEEIRRLPVVDKT
                     GSLVGIVTIGDIRSANPSPATSLSIWEMNYLLTNLKIEKIMTRNPQTIRADATIGEAA
                     RTMLEHRVSGLPVVDADHHVVGIITESDIFSMVVLHEWSIEHS"
     gene            complement(4867..5235)
                     /locus_tag="PLC06_11380"
     CDS             complement(4867..5235)
                     /locus_tag="PLC06_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRO91090.1"
                     /translation="MGWYERLISRNVPNISPAEAYKRLQGDAKVVIVDVRQPMETASG
                     TIPGAVLIPLSEFGRRMAELPRDRPILTICRSSHRSPIAARQLKRAGYDVTNVSGGTL
                     AWQRAGLPFTHGDEGGAGAG"
     gene            5348..>5705
                     /locus_tag="PLC06_11385"
     CDS             5348..>5705
                     /locus_tag="PLC06_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRO91091.1"
                     /translation="MSDNGAALSAHRQDPNPMTNQPDPTLHDLVIKNGTVISDAAVFV
                     ADVAVDGEQIAAIGHDLRGRRTIDADGLYVIPGAIDIHVHLEMPIGRFTSTDDFYHGT
                     LAAAMGGTTAIVDFVET"
BASE COUNT         1129 a         1894 c         1701 g          981 t
ORIGIN      
        1 gacgactaca cctccaccga gcatctgctc atggccatgg cccagcccaa gggcggccgc
       61 gtccgcgagc tgttggcccg ccacggcatc gactacaacg ccatcctgca agcgctggcc
      121 gccgtgcgcg gctcgcagcg cgtcaccagc cagaaccccg aagcgcaata cgaggcgctg
      181 gccaaatatg gccgcgacct gacccaggaa gcgcggcgcg gcaagctcga cccggtcatc
      241 ggccgcgacg aggagatccg ccgcgtcatc cagattctga gccgccgcac caagaacaac
      301 ccggtgctca tcggcgagcc gggcgtgggc aagacggcca tcgtcgaggg gctggcccag
      361 cgcatcgtca acggcgacgt gccgctgggc ctcaaggaca agcggctcgt ctcgctcgac
      421 ctgggcgcgc tggtggccgg ggccaagtat cgcggcgagt tcgaggagcg gctcaaggcc
      481 gtcctcaagg aggtgcagga agccgaaggg cagatcatcc tgttcatcga cgagatgcac
      541 accctgatcg gcgcgggcgc ggccgaaggg gccatggacg ccagcaacat gctgaagccg
      601 ctgctggctc gcggcgaact ccacgccatc ggcgcgacca cgctcgacga gtatcgcaag
      661 cacatcgaga aggacgccgc gctggagcgc cgcttccagc cggtattcgt cgatgagccg
      721 tcggtggagg acaccatctc catcctgcgc ggcctgaagg agcgctacga ggtccatcat
      781 ggcgtgcgcg tcaccgacgc ggccgtcatc gccgccgcca cgctgagcca tcgctacatc
      841 accgatcgct ttctgcccga caaggccatc gacctcatcg acgaggccgc cagccgcctg
      901 cgcatgcaga tcgacagcaa gccggccgaa ctggacgaga tcgatcgcgt catcatgcag
      961 ctggagatcg agcgcgaggc ccttaagaaa gagaaggaca aggccagcca ggagcggctg
     1021 ggccggctcg aagaagagct ggccaacgcc aaggagcgca gcatccagct gacgggccgc
     1081 tggcaggcgg agaaggacgc catccagcac atccagaccg tcaaggaaga gatggacgcg
     1141 gtgcgcatcc aggtggagca ggcccagcgc gattacgact accagaaggc gtcggagctg
     1201 caattcggcc gcatgcgcca gctggagcac gacctgagcg aggccctggc ccaactgcat
     1261 cagattcagg ccgacggcgc gatgctgaag gaagaggtcg acgccgagga catcgcctac
     1321 atcgtcagca aatggacggg catccccgcc accaagctgc tggagggcga ggtcgagaag
     1381 ctcatctaca tggaagatcg gctccaccgg cgcgtcgtgg gccaggaaga ggccgtcgcc
     1441 gccgtggccg ccgccgtgcg ccgcagccgc tccggcctgc aagaccccaa ccggcccatc
     1501 ggctcgttca tctttctggg gccgacgggc gtgggcaaga cggaactggc gcgggcgctg
     1561 gccgaattcc tgttcgacga cgagcgcagc atggtgcgca tcgacatgag cgaatatcag
     1621 gagcgccaca ccgtggcccg gctcatcggc gcgccgcccg gctacgtggg ctatgaggaa
     1681 ggcggccaac tgaccgaggc cgtccgccgc cgcccctaca gcgtgatcct gttcgacgag
     1741 atcgaaaagg cccacccgga ggtgttcaac gtcctgctgc aagtgctcga cgacggccgc
     1801 ctgaccgacg gccaggggcg cacggtcgac ttccgcaaca cggtcatcat catgaccagc
     1861 aatgtgggca gccagttcat cctgggcgat ctggacgagg agacgatgcg cgcgcgggtc
     1921 atggaagcgc tgcgcgccca gttccggccg gagttcctca accgggtgga cgagatcgtc
     1981 atcttccacc ggctcggccg cgagcaattg cgcgagatcg ccgagattca gctcaacaat
     2041 gtgcgcggcc tgctggccaa acgcgacatc acgctggcgc tgaccgagac ggccaccgat
     2101 ctgctgatcg aagaaggcta cgacccggtc tatggcgcgc ggccgatcaa gcgcgtgctg
     2161 cagcggcgca tcatcgaccc gctggccatc cgcgtcatcc agggggacgt gcgcgacggc
     2221 gaccacgtgg tggtcgaggt catcgacggc caactggatt tccgggtggt ggagtcgggg
     2281 gagtttgaga cggcgtagtc atcggatcaa tcaataacaa gggcgctgca tcgagcacgg
     2341 atgcagcgcc ttttctcttt ctgctataat ggggtcagac aactcgaagg aagatgcgcc
     2401 atgtccattt actcaattcg cctctatctg gaaccgaatc cggatggtca attcaccgtc
     2461 acaagtcccg atgtacccgg tctggtcact gaaggggaca cgcctgaaac gatccaggct
     2521 aatgtccagg aggctctgga cgcgctgctt gacgaagatt acttccgtca ggtgtcgatc
     2581 gatagcgctg ggggaatcgt ctggcccaat gactatgact tttgccccaa cttcctgtac
     2641 gaggatgttc ccgctgttga gtccgtggcg tcgtagtatg atataatttg ggtcagctcg
     2701 gaccttgagc aaaaagcaat ggacaccgtc accaaaataa aacgaacggt acgaatggaa
     2761 ggtaccccct tgatcccccc gctccaaacc ggcgaccacc tcacgcgggc cgaattcgag
     2821 cggcgctatt ccgcgcaacc ccatctcaag agggccgagt taatcgaagg agttgtctac
     2881 gtgccgtctc ccgttcatct ggactctcat gctcgccccc atagccgtat tatgggcttg
     2941 ttattcaact atatggccgc cactccgggg acagacacgg ccgacaacgc cagcgttcgc
     3001 cttgacgccg agaacgaggt ccagccggat gctatgcttc gtattcggga agagtatggc
     3061 ggccaatcgc gccaaagtga agatgattat ctgatcggcg cacccgaact cattgtcgaa
     3121 atcgccgcca gcagcgccgc ctacgatctg cacgagaagc tgcgtgtcta ccgtcgaaca
     3181 ggcgttaaag agtacgttgt cttgcttacg ttggaactcg aaacagtttg gtatcgcctg
     3241 aacgaaggtc gctatgacgt ggtatcgccg gatgaggacg gcgtctatcg cagcgctgtc
     3301 ttccccgggc tgcatttcca ctcggccaat ttctgggctg acgacgtggc gggccaactg
     3361 gccgttctgc aggcgggcat tgcgaccccg gcgcatgccg atttcgtcgc cgccctgcgc
     3421 cctgtctgat ccacttcaca ccccatcaaa ctccggccga tagcgcacca ccccccgcgc
     3481 cccggccagc gcccccggtc gcaattccat gaccatgaac gcctcctccg gcgcgtcata
     3541 ctcccagcgg atacccagcg gccgcgacgg gcgaaagccg aagcgcgggt aatacgtcga
     3601 gtggcccagc acgatggcca gcccgtagcc ctgctcgcgg catatatcca gcccggcgcg
     3661 aatcagcgcg tcgccgatgc cgcgccgctg ccggtcgggc agaacggcca ccggccccag
     3721 cgcggccact gacgtaaggc ggccgtcatc gtcgagcgtg gccgggctgt agaggacgtg
     3781 gccaacgacc tgccccgcgt cctcggccac caacgagcag atgacggctc tgctttcccg
     3841 cagcgcatcg accagcgccc cctcgtcctc gcggccgaac gccggccggt tcaccagccg
     3901 gatcgcagcc acatcatttg gggtctcggc gcgaatgacc atttaatccc tcactcataa
     3961 tagaacatcg atccgaaaaa attctgtgaa tttctcatca ttccgtaagg ttgttcgtaa
     4021 ggattgtaac ctattttata gatgctcctt actccttccc accccgatgg gaactgttca
     4081 tttttaggat ggcgccattt cccaaggtgg cgccatcccc ctccgttttt gtcctgtcga
     4141 tcatggcaaa tatcacatcc cacatgtgac gtttgtaact atcttttccg atccctcgtc
     4201 ccgtataatg ggaacaacat cgagaccggc cacgccattt ttgggccgcg gccgtggaga
     4261 aatgcagcca tgaaaaaaga cctcgtccag gactggatgt cgacggacgt tatcactatc
     4321 gcgccggaca cgccgctgcc cgaagcgcac aatatcatga ccaccgagga gattcgtcgc
     4381 ctgccggtgg tggacaagac cggcagtctg gtcggcatcg tgaccattgg cgatattcgc
     4441 agcgccaacc cgtcgccggc cacctctctc agcatctggg agatgaacta tctgctgacc
     4501 aatctcaaga tcgaaaagat catgacccgt aacccccaaa ccattcgcgc cgacgccacc
     4561 atcggcgagg cggcgcggac gatgctggag catcgggtca gtggcctgcc cgtggtcgat
     4621 gccgatcatc acgtcgtcgg catcatcacc gagtcggaca ttttcagcat ggtggtgctc
     4681 catgagtgga gcatcgagca ttcctgagca agccggctgc cggtgaccgc cgcaaggtgg
     4741 cttcccgtca gtcgtaccac ctctgggaga gggccaggcg cgtcgggttg gcgcgccaac
     4801 ttataaccgg cggggtgact atcctccgct aattttagcg tcaccccgcc ggttttctgt
     4861 gcctcgttat cccgcgcccg cgccgccctc gtcgccatgg gtgaatggca gacccgcccg
     4921 ctgccaggcg agcgttccgc cgctgacatt ggtgacgtcg tagcccgcgc gcttgagctg
     4981 tcgagcggcg atggggctgc gatggctgga gcggcagatg gtcaggatcg gccgatcgcg
     5041 cggcagctcc gccatccggc ggccgaactc cgacaacgga atcagcaccg cgccggggat
     5101 cgtccccgaa gccgtctcca tcggctggcg cacatcgacg atgacgactt tcgcgtcccc
     5161 ttgcagccgt ttgtaggcct cggccggact gatattgggc acattccggc tgattaatcg
     5221 ttcataccat cccattttcg ctcctttgcc gtagcgcaat ccgccggatt gcttccgggt
     5281 cgcaatccgg cggattgcgc tacaactatt gcacctatta tatataacca ttggcgaatc
     5341 accccgcatg agcgacaatg gcgctgcctt gtccgcccac cggcaagacc cgaatcccat
     5401 gaccaaccag cccgacccca ccctccatga tctcgtcatc aaaaacggca cggtgatcag
     5461 cgacgcggcc gtcttcgtcg ccgatgtggc cgtcgacggc gaacagatcg ccgccatcgg
     5521 ccacgacctg cgcggccgcc gcacgatcga cgccgacggc ctctacgtca tccccggcgc
     5581 catcgacatc cacgtccacc tggagatgcc catcggccgc ttcacctcca ccgacgactt
     5641 ctaccacggc accctcgccg cggccatggg cggcacgacg gccatcgtcg acttcgtgga
     5701 gaccc
//
LOCUS       DAOMQG010000215         5699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5974_length_5699_cov_11.948795, whole genome shotgun sequence.
ACCESSION   DAOMQG010000215 DAOMQG010000000
VERSION     DAOMQG010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5974_length_5699_cov_11.948795"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1169
                     /locus_tag="PLC06_11390"
     CDS             201..1169
                     /locus_tag="PLC06_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91092.1"
                     /translation="MTRMTHAVARHLQDEFDCRFSPFYADGLYDFLATHTKLVNFTAL
                     GGNFRRQTDAYLRLHDLPVDYRGQTGDYDLYVVTTDLVVPRNLKDKPFILIQEGMTDP
                     EGIMYHIVRKLRLPRFLASTSTNGLSQQYRYFCVASEGYRDFFIKKGIQPEKLLVTGI
                     PNFDHSAACLDNDFPLRDYVLVATSDARETYKFDNRKKFLRNAVQLAAGRPMIFKLHP
                     NEKFDRAIREIHDVVGDVPIYTEGNTDHMIANCVALICQYSTIAYTGLALGKEVHSYF
                     DLEMLRRLAPMQNGGTSGRNIAQVCRHILRNEPVSWEEFLTHDSTD"
     gene            1153..1968
                     /locus_tag="PLC06_11395"
     CDS             1153..1968
                     /locus_tag="PLC06_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRO91093.1"
                     /translation="MIRPTDGIVTVIQARTGSTRLPNKVLRPLAGKPLLVRMVERVAA
                     ARLVGTVVVATTREPADDPLEALCRDAGLNCYRGDSSDLLDRHYQVGKLYQASAVIKI
                     PSDCPLIDPRIIDRVIAHYLAHRGGYDFVSNLHPATYPDGNDVEIMSMPALEIAWREA
                     RRPLEREHTTPFLWENPDRFRLANVPWETGLDYSMSHRWTIDYEADYQFIAAVYDALY
                     SSERHFTLEEILAFLSTHPEIGAINQRYAGVNWYRHHLDDLKTVSAEQTRQES"
     gene            2029..2886
                     /locus_tag="PLC06_11400"
     CDS             2029..2886
                     /locus_tag="PLC06_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRO91094.1"
                     /translation="MNQEELSELENMALRVREHIIRLSTDGGCFTGASLSCADLIVYL
                     YTHFLNVGPDNFDALDRDYLFLSKGHDVPALYGTFVELGWMEPERLRNHLKTNDTIYW
                     HPNRNIPGIEFHSGSLGHLPSVAVGVAMDCKMRGLDNRIVVITGDGELNEGSVWEAIL
                     VANAYRLDNLLFIVDRNQFQANIRTEELIPLEPLAPKFEAFGWKARRVNGHSFAELDD
                     ALAKVPFAGGRPSVLIADTVRGRGLPSIQERADRWFCNFTHEEVDALLEELHGVRRAE
                     IESEALMVR"
     gene            2927..3838
                     /locus_tag="PLC06_11405"
     CDS             2927..3838
                     /locus_tag="PLC06_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRO91095.1"
                     /translation="MKNYEQTLLDMMAADERIIVMTAENRAAIRNLPKLAPERFIDTG
                     ITEQTMVGAAAGLALRGRVPVLHALATFLTLRAFEFVRTDVGLGNLPVKLVGGVPGFL
                     SDGNGPTHQAIEDVSLMRGIPHMHVFCPADEQDMLLGLPHIINSPHPTYIRFNALKPV
                     IDHDSEFEIGRAEQVSDGTDVAILVYGMLFAQAWEAKALLEAEGLSVRLINVRMPKPI
                     DEEAILRAARETNLLVTLEDHFLTGGLYSIVAETLLRHGETAEVLPFALEERWFRPAL
                     LNEVLRFEGFTGEQIAARILERVPAHA"
     gene            3936..5270
                     /locus_tag="PLC06_11410"
     CDS             3936..5270
                     /locus_tag="PLC06_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRO91096.1"
                     /translation="MPNKVQLNQDYPIITESEKLFERAKGLMPAFSQTLAKGPTQYVD
                     GVAPKYLRRAKGAHVWDVDGNEYLDYNSAIGPISLGYGYDVVDDAVRRQLEDGIAFSL
                     IHPLEVEIAELVREVIPNAESVRYAKAGAEVTSAAVRLARAYTGRDKVLCCGYHGWHD
                     WYIGITARNEGVPQAVKDLVFTFNYNDMDSVMAALDEDVACVILEPMTFDFPKDDFLT
                     RLKTACEANGTLLIFDEMWTGFRWALGGAQEYFDVTADLACYSKAIANGMPLAVLAGR
                     ADVMSLLDEHVFFFTTFGGEALSLAAAGATIRELRDKNVPADLARKGQKLMDGYNEIA
                     SELGIRDITYCKGHPARGMIVYDDKAGRSLLMKSLVQQELIKRGILWSGFHNMCYSHS
                     EADLDYTLIAYGEALAELKAALEAGDIAARLRGTPVQPVFRRTDSFNLKPAR"
BASE COUNT         1219 a         1817 c         1560 g         1103 t
ORIGIN      
        1 ttcattcgca ttcattcgca ttcattcgca ttcattcgca ttcattcgca ttcattcgcg
       61 ttcattcgcg ttcattcgcg gtccaatatt cttcatgcga ttgccctgta gcgcgccctg
      121 ttggactccc ccggcttagg cgataaacta ccccggctat ggcacggaaa aagatactgt
      181 tcatcggagg gtcgctgaat atgacgcgga tgacccacgc cgtcgctcgt cacttgcagg
      241 acgagttcga ctgccggttc agtccgttct acgccgacgg actctacgac ttcctggcca
      301 cccacaccaa attggtcaac ttcaccgccc tggggggcaa cttccgccgc cagaccgacg
      361 cctatctacg cctccacgac ctgcctgtcg actatcgcgg gcaaacagga gattacgacc
      421 tctacgtggt cacgacggac ctcgttgtcc cccgcaatct gaaggacaaa ccgttcatcc
      481 tcatccagga gggcatgacc gatcccgaag ggatcatgta ccacatcgtg cgcaaactca
      541 ggctgccccg ctttctggcc agcacctcga ccaacgggct gtctcagcaa taccgttact
      601 tctgcgttgc ctcggaaggc tatcgcgatt tcttcatcaa aaaaggcatt cagcccgaaa
      661 agctgctcgt caccggcatc cccaacttcg accatagcgc cgcctgcctg gacaacgatt
      721 tccccctgag agattatgtc cttgtggcca cgtccgacgc tcgcgagacg tataaattcg
      781 acaaccgcaa gaagtttctg cgaaacgcgg tccaactggc cgcgggacgg ccgatgatct
      841 tcaagcttca ccccaacgag aagttcgacc gcgccattcg cgagatccac gacgtcgtcg
      901 gcgacgtccc catttatacc gaaggcaaca ccgaccacat gatcgccaat tgcgtcgcgc
      961 tcatctgcca gtattcaacg atcgcctata ccggcctcgc tctgggcaag gaagttcatt
     1021 cctacttcga cctggaaatg ctgcgacggc tggccccaat gcaaaacggc ggcacatcgg
     1081 gccggaacat cgcccaggtt tgccgccata ttctgcggaa tgaaccggta tcatgggagg
     1141 agttcctgac gcatgattcg accgactgac ggcatcgtca ccgtcatcca ggctcggaca
     1201 ggatcgacgc gcctgcccaa caaggtactt cgccccctgg ccggcaagcc gctgctggtg
     1261 cggatggtcg agcgggtggc ggccgcccgg ctcgttggga ccgtggtcgt ggcgacgacc
     1321 cgcgaaccgg ccgatgatcc gttggaagcc ctgtgccgtg acgcgggact caattgttat
     1381 cgtggcgatt ccagcgacct gctcgatcgc cattatcagg tcggcaagct ctatcaggcc
     1441 tcggccgtca ttaaaatacc ctcggactgc cccctcatcg atccgcgcat catcgaccgg
     1501 gtgatcgccc actacctggc gcatcgggga ggctatgact tcgtcagcaa tctgcacccg
     1561 gccacctatc ccgacggcaa cgacgtagag atcatgagca tgcccgcgct ggagatcgcc
     1621 tggcgcgagg cccggcggcc gctggagcgc gagcatacca cccccttctt gtgggagaat
     1681 cccgatcgat tccgcctcgc caatgtcccc tgggagacag ggcttgacta ctccatgagc
     1741 catcgctgga cgatcgacta tgaagcagat taccaattta tcgccgccgt ctatgatgcg
     1801 ctatactcaa gcgagcgtca tttcacgctt gaggaaattc tcgcctttct gtccacacac
     1861 ccggagatag gggcgatcaa tcagcggtat gcgggagtca actggtatcg ccatcacctc
     1921 gacgatctta aaacagtttc cgccgagcag acgcggcagg aatcatgact gacctttgac
     1981 cacaagaacg acacctttca cccttgatct ccatagggaa ggaaaagtat gaaccaagag
     2041 gaactatctg aactggaaaa tatggcgctg cgcgttcgcg agcatatcat ccgcctgtca
     2101 acggacggcg gctgcttcac cggcgcgtcc ctgtcctgcg ccgatctcat cgtgtacctg
     2161 tacacccact tcctcaatgt gggtccagac aactttgacg ccctcgatcg ggactatctg
     2221 ttcctctcga aaggccacga cgtccccgcc ctctacggca ccttcgtcga actgggctgg
     2281 atggaaccgg agcgtctgcg caatcatctg aagaccaacg acaccatcta ctggcatccc
     2341 aatcgcaaca ttcccggcat cgaattccac tccggctcgc tggggcatct gccgtcggtg
     2401 gctgtcggcg tggccatgga ctgcaagatg cgcgggctgg ataaccgcat cgttgtcatc
     2461 accggcgacg gcgagctgaa cgaaggctca gtctgggaag ccattctggt ggccaacgcc
     2521 tacagactgg acaacctgct gttcatcgtc gatcgcaatc aattccaggc caacatccgc
     2581 accgaagaac tgatcccgct ggagccgctg gcgcccaagt ttgaggcgtt cggctggaaa
     2641 gcccggcgcg taaacggcca cagcttcgcc gaactggacg acgccctggc caaagtgccc
     2701 ttcgccggcg ggcggccgag cgtgctcatc gccgacaccg tccgcggccg cggcctgccc
     2761 agcatccagg aacgggccga ccgctggttc tgcaacttca cccatgaaga agtagacgcg
     2821 ctgctggaag aactccacgg tgtgcggcgg gccgagatcg aatcggaagc attgatggtc
     2881 agataagagg agaatccacg gattagcctt tttgatgagc aatattatga agaactacga
     2941 acaaacctta ctggacatga tggccgccga cgagcgtatt atcgtcatga cggccgaaaa
     3001 ccgggccgcc atccgcaacc tgcccaaact cgcgccggag cgtttcatcg ataccggcat
     3061 caccgagcaa acgatggtcg gcgcggccgc cggtctggcc ctgcgcggcc gcgtgcccgt
     3121 tctccacgcc ctggccactt tcctgaccct gcgcgcgttt gagttcgtgc gcaccgacgt
     3181 gggcctcggc aatctgccgg tcaagctggt gggcggcgtg cccggattcc tctccgacgg
     3241 caacggcccc acacaccagg ccatcgagga cgtctcgctc atgcgcggca tcccccatat
     3301 gcacgtcttc tgcccggccg acgagcagga catgctcctt gggctgcccc acattatcaa
     3361 cagcccccac cctacttata tccgctttaa cgccctgaag ccggtcatcg accatgatag
     3421 cgagttcgag atcggccgcg cggaacaggt gtcggacggc accgatgtcg ccattctggt
     3481 ctacgggatg ctctttgccc aggcatggga ggccaaggcg ctgctggaag ccgagggcct
     3541 ctccgtccgg ctcatcaacg tgcgcatgcc caagccgatt gacgaggaag ccatcctgcg
     3601 cgcagcgcgc gagacaaacc tgctggtgac gctggaagac catttcctga ccggcggcct
     3661 gtactccatc gtggccgaga cattgctgcg ccacggcgag acggccgaag tcttgccgtt
     3721 cgccctcgag gagcgctggt tccgtccggc tctgttgaat gaggtgttgc gcttcgaagg
     3781 cttcaccggc gagcagatcg ccgcgcgcat ccttgagcgc gtgcccgccc atgcctaatc
     3841 tgatgtagca ccggtttcct aaccggtgga tatagacgtt acgaattctg aagatgccgg
     3901 ttaagaaacc ggcgctacat ttaaggaaaa atcaaatgcc gaataaagtc cagctcaatc
     3961 aggactatcc aatcataacc gaatccgaaa aactcttcga gcgggccaaa gggcttatgc
     4021 cggccttctc ccagacattg gccaaaggcc ccacgcaata tgtcgacggc gtcgcgccca
     4081 aatatctgcg acgtgccaaa ggcgcccacg tctgggacgt ggacggcaac gagtacctcg
     4141 actacaactc agccatcggc cctatttcgt tgggctacgg ctatgacgtc gtcgatgacg
     4201 ccgttcggcg gcagctcgag gacggcatcg ccttctcgct catccacccg ctggaggtgg
     4261 agatcgccga gctggtgcgc gaagtgatcc ccaatgccga gagcgtgcgc tacgccaagg
     4321 ccggtgccga agtcaccagc gccgccgtgc gcctggcccg cgcctacacc ggccgggaca
     4381 aggtgctctg ctgcggctac cacggctggc atgattggta catcggcatc accgcccgca
     4441 atgaaggcgt gccgcaagcc gtgaaggatc tcgtattcac tttcaactac aacgatatgg
     4501 attcggtcat ggctgccctt gatgaggacg tagcctgcgt cattctggag ccgatgacct
     4561 tcgacttccc caaggacgac ttcctcacca ggctcaaaac ggcatgcgag gccaacggca
     4621 cgctgctcat cttcgatgag atgtggaccg gcttccgctg ggcactaggc ggcgcgcagg
     4681 agtatttcga cgtgacggcc gacctggcct gctactcaaa ggccattgcc aacggcatgc
     4741 ccctggccgt gctggccggc cgcgccgacg tgatgagcct gctcgatgaa cacgtcttct
     4801 tcttcaccac tttcggcggc gaagcgctct cgctggccgc ggccggcgcg actatccgcg
     4861 aactgcgcga caagaatgtg cccgccgacc tcgcccgcaa ggggcagaag ctgatggacg
     4921 gctacaacga gatcgcctcg gagctgggaa tccgcgacat cacttattgc aaaggccacc
     4981 cggcgcgcgg catgatcgtc tacgatgaca aggcgggccg gagcttgctg atgaaatcgc
     5041 tcgtgcagca ggaactcatc aagcgcggca tcctctggtc cggcttccac aacatgtgct
     5101 acagccacag cgaggcagat ctcgactaca cgctgatcgc ctacggcgaa gcgctggccg
     5161 aactcaaggc agcgctcgaa gcgggcgaca tcgcggctcg cctgcgcggc acgccggttc
     5221 agccggtgtt ccgccgcacc gacagcttta acctgaagcc ggcgcggtga tagtggatca
     5281 gtggatcagt aggtcagtag gtcagtaagt cagtgggtca gtgggtcagt gggtcagtgg
     5341 gtcagtaaga cagtaagaca gtaggtcagt aagacagtaa gacagtagga cagtaggaca
     5401 gtagggcaac agattaggcg aaacagggca ctgaacactg atccactgaa tactgatcca
     5461 ctgaacactg atccactgaa tactgatcca ctgaacactg atccactgaa tactgatcca
     5521 ctgaatactg atctactgaa tactgatcca ctgaatactg atccactgaa cactgatcca
     5581 ctgaatactg atctactgaa cactgatcca ctgaacactg atctactgaa tactgataat
     5641 aagtttcatc aacatgatcg acacaactct cttctctctt gagggcaaga cggccgtcg
//
LOCUS       DAOMQG010000216         5684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_5995_length_5684_cov_9.402381, whole genome shotgun sequence.
ACCESSION   DAOMQG010000216 DAOMQG010000000
VERSION     DAOMQG010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5995_length_5684_cov_9.402381"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLC06_11415"
     CDS             complement(<1..1226)
                     /locus_tag="PLC06_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HRO91097.1"
                     /translation="MTSASNLDKREATSVATTESQGGEWVQAETPAAHREAIAALPFR
                     QPPAPATEWLLHGEAPAEGQPLVLMHQTAILQVIAHSRSNTDYELGGALLGHVYRYKK
                     GIVVEVKAALPATSADHGPVHFTFTADAWSKLHQDRAAHYSNLDIIGWFHTHPDLGVF
                     YSSDDVVVHSAAFTLPWHVGLVVDPVREEAAYFGWVAGSLTPLAGFYELPERQPNTLV
                     PWRYVRTSVWDRPYEMQPPTDEHSRVVLARSGRPRFSGRELGLAASAAALLLSFFLIV
                     GGILPLQRRVNLLETTVLNLAQASLTGSNGLTCPDPRLRILTPLTGQTYIAGETLQII
                     GTAEYPGARRYQLEYRPAGNNEWKLINRPRKDMRLDLLAEWDTTGLSVGSYELRLSPV
                     DTNNIRLANSSPCTIIV"
     gene            complement(1201..1734)
                     /locus_tag="PLC06_11420"
     CDS             complement(1201..1734)
                     /locus_tag="PLC06_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HRO91098.1"
                     /translation="MPFDIRETRLRNDYQRVRDLANRSDFIHIVRTEGDPPEKYLIRY
                     TCKGVEGISGSNQPIFREQHDVTIYLHAEYPLKQPQLKWVSPIFHPNIHVTGAVCIGA
                     WWPAKTLDELLLTLGEMIQYKNYEPRDPMNSKAAAWAMQRKSLFPIDRRELKGQSVAD
                     LIVIRDEEPDDLGIKFG"
     gene            complement(1927..2193)
                     /locus_tag="PLC06_11425"
     CDS             complement(1927..2193)
                     /locus_tag="PLC06_11425"
                     /inference="COORDINATES: protein motif:HMM:NF020396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HRO91099.1"
                     /translation="MASTKVIIYDPTGSKKTPVELPDDVPMRRLIPALVSKMGLPTTQ
                     GANPITYRLDHRSSGKRLGDEESLRDAGVKEDDILSLFPEVTAG"
     gene            complement(2302..3483)
                     /locus_tag="PLC06_11430"
     CDS             complement(2302..3483)
                     /locus_tag="PLC06_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRO91100.1"
                     /translation="MTEPETIVITDPDSDRYHTFGYISWWQQEVVRNATVLVIGAGAL
                     GNEVIKNLALMGIGNILIADFDTIEDSNLSRSVLFRTTDRGRRKVDAAAEAVKALNPD
                     VKVKAWHGNINFEMGLGVFRHVDVIVGCLDNREARLSINRFSWAINRPWVDGAIQELM
                     GIVRVFRPGEGACYECTLTDMDYQIINLRYSCPLLARQDILMGKVPTTPTSASIVAAF
                     ETQEALKLIHNMEVQPGKALMINGLTNDIYMTEYPVKEFCMSHSRLEPIVELPEATAA
                     GTTLAEMLANARERLGDGAVLEFDSELVVSMACGACGNVEPIFKRMARLYEDEAICPN
                     CGAKREMNLTHRISGDEDFLERTFAEMDVPPLGIIRARNGSERVYFELTGDKETFLQF
                     A"
     gene            complement(3500..4153)
                     /locus_tag="PLC06_11435"
     CDS             complement(3500..4153)
                     /locus_tag="PLC06_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRO91101.1"
                     /translation="MSDVDPLRITAEDIEAQRGLVMAPPVLTISLEELPDIATLPAAP
                     RSHTLHVTAEDIAAFEPASEEVVGALEQLSFRLVNQAREEHLPSWLGRGPLQWHPQLA
                     LVARQHANDMLDRRYVAHTTPEGVAVANRLDRRAISYIACGENIGVVYGPASHGERGV
                     QEVHFAFMNQPRRLTNHRANILNPVWTHVGIGVAYAPEGQLIVTQNFLATLTKPVQT"
     gene            complement(4166..5389)
                     /locus_tag="PLC06_11440"
     CDS             complement(4166..5389)
                     /locus_tag="PLC06_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91102.1"
                     /translation="MHETAQKQVQAFAGKGQMAAFAGRLLEWLNRDLNPNGESLTESR
                     ARAILLAANVLDEPTRHSFAQAVESEQGMRLALYGLLRDSGLADKEDVAVLAATSAGV
                     MPQSEQSAPSWLALAVAAFAWRADFLLDRLDPAAPPDPFSPAGQVLKRAAYFIRQEVQ
                     RSATERDKLGRKLAHAEGGAPSLNALRPDGTIAPLPPYYRMPIPERYPEYNPEIGVSP
                     DEIEHPAAPAPGAPLSITNADLSSGPSAPTTQPPLRIDISADEPPLQPTTQRPATHPP
                     AAPRPAHVVMPKATTTANTAGASAAIRGGRGRNAMKATKLQVVVQERPNGPGLYGLQV
                     RISSRGIRRYVAGTTNKDGEFICELPVQLDAGLTYDVDVTWPRDFGGEVERKSITLNA
                     DRTHFTLPFYRKLKD"
     gene            complement(5457..>5684)
                     /locus_tag="PLC06_11445"
     CDS             complement(5457..>5684)
                     /locus_tag="PLC06_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91103.1"
                     /translation="DLLPRLRRLFWLEAIKTERDFNNLLVKPMLRQHDRNLHKPELRK
                     ALEVDMESVSDIEELMRVWDGLHHLETGLAI"
BASE COUNT          960 a         1705 c         1881 g         1138 t
ORIGIN      
        1 acgatgatgg tgcaggggct gctgttcgcc aggcggatgt tatttgtgtc aaccggcgac
       61 aagcgcaatt cgtagctccc gacgctcaat ccggtggtat cccactccgc cagcaggtcg
      121 agccgcatat cttttcgcgg cctgttgatt agtttccatt cgttattgcc tgcgggccgg
      181 tattcaagct ggtagcgccg cgcgccgggg tattcggccg tgccgatgat ctgaagcgtc
      241 tcgccggcga tgtaagtctg cccggtcagc ggggtcagga tgcgcaggcg cgggtcgggg
      301 caggtgaggc cgttgctgcc ggtgagcgag gcttgcgcca gattaaggac cgtcgtctcc
      361 agcaggttca cccgcctttg aagcggaaga atcccgccga cgattaaaaa aaaagacagc
      421 aataatgcgg cggccgaggc cgccaggccc aactcgcggc ccgaaaatcg cggccgcccg
      481 ctgcgcgcca ggacgacgcg actgtgttcg tcggttgggg gttgcatctc ataaggccga
      541 tcccacaccg atgtgcggac gtatcgccac ggaacaagcg tgttcggttg tcgctcaggc
      601 agttcgtaga agccggccag aggggtcagg ctgcccgcaa cccagccgaa atacgccgct
      661 tcctcgcgca cgggatcaac cacgaggccg acatgccagg gcagcgtgaa ggccgccgaa
      721 tgcacgacga cgtcgtcgct ggaatagaac acgcccaggt cggggtgggt atggaaccag
      781 ccgatgatgt cgaggttgct gtaatgggcg gcgcggtcct gatgcagttt cgaccaggca
      841 tcggccgtga acgtgaaatg aaccggcccg tggtcggcgc tggtggccgg cagggcggct
      901 ttcacctcaa ccacgatccc tttcttgtaa cggtagacgt ggcccagcag cgcgccgccc
      961 aactcgtaat cggtgttgct gcggctgtgg gcgatgactt gcaggatggc cgtctggtgc
     1021 atgagcacca gcggttggcc ttcggccggc gcttcgccgt ggaggagcca ctcggtggcc
     1081 ggggcgggcg gctggcgaaa cggcaaggct gcgattgcct cgcgatgggc cgccggcgtc
     1141 tctgcctgca cccattcgcc gccttgcgat tccgtggtcg ccaccgacgt cgcttcgcgc
     1201 ttatccaaat ttgatgccga ggtcatcggg ttcctcatca cggatgacga tcaggtcggc
     1261 cacggattgc cccttgagtt cgcggcggtc gatggggaag agcgacttgc gctgcatggc
     1321 ccaggcggcc gccttggagt tcatggggtc gcgcggctcg taattcttgt actgaatcat
     1381 ttcgcccagc gtcagaagga gttcatccag cgtcttggcc ggccaccacg cgccgatgca
     1441 caccgcgccg gtcacgtgga tgttgggatg gaaaatgggg gatacccact tgagttgggg
     1501 ctgcttcagc gggtattcgg cgtgcaggta gatggttaca tcgtgctgct cgcggaatat
     1561 cggctggttc gatcccgata ttccctccac acccttgcag gtgtaacgga tcagatactt
     1621 ctccggggga tcgccctcgg ttctgacgat atggatgaaa tcgctccgat tcgccagatc
     1681 gcgcacgcgc tggtagtcgt tgcgcaatcg ggtttcacga atgtcaaaag gcatggttgc
     1741 tcctgttttt gctgataatc aagtgccctt cgcggtgttc gtccgggccg gcgcttgtcg
     1801 gcaaccggcg aaccacggga agggcacgat catctgcgaa atcaacgcgc gttcgcgtcg
     1861 gttggttcgg atgaaggcgc ttgtggcgct atgaccgggg tggcccggct ccgaacggcg
     1921 taccgattag ccggccgtaa cttcggggaa caggctgagg atgtcgtcct ctttcacgcc
     1981 ggcatcccgc aacgattcct cgtcgccgag ccgcttgccg gaagaacggt gatccagccg
     2041 gtaggtgatc ggattcgcgc cctgcgtcgt cggcagaccc atcttgctca ccagagcggg
     2101 gatcaaccgg cgcattggaa cgtcatccgg caactcgacc ggggtcttct tggacccggt
     2161 gggatcgtaa atgattacct tggtactcgc cattttttca aaagcctcca tcaatagatt
     2221 cgtaacacat tacgcaaaat caatgttgcg gttgcaacgc gttcccgttg tttgcggggc
     2281 gggccgccgc ttgatcagaa ctcaggcaaa ctgtagaaaa gtttccttgt cccctgtcaa
     2341 ctcaaagtag acccgctcgc tgccgttgcg ggcgcgaata atgcccagcg ggggcacgtc
     2401 catctcggcg aaggtgcgtt cgagaaagtc ctcgtcgccg ctgatgcgat gggtcaggtt
     2461 catctcgcgc ttggccccgc agttggggca gatcgcttcg tcctcgtaga gccgggccat
     2521 gcgcttgaag atcggctcga cattgccgca ggcgccgcag gccatcgaca cgaccagctc
     2581 gctgtcgaac tccagcacgg ccccatcgcc cagtcgttcg cgggcgttgg ccagcatctc
     2641 ggccaatgtc gtcccggcgg ccgtggcttc cggcagctcc acaatcggct ccagccggga
     2701 gtggctcatg cagaactcct tgaccggata ttccgtcatg tagatgtcgt tggtcaggcc
     2761 gttgatcatg agcgccttgc cgggctgcac ttccatgttg tggattagct tgagcgcttc
     2821 ctgcgtctcg aaggcggcca cgatcgaggc gctggtcggc gtggtgggca ccttgcccat
     2881 caggatgtcc tggcgcgcca gaagcgggca ggagtagcgc agattgatga tctggtaatc
     2941 catgtcggtg agggtgcatt cgtagcaagc gccctcgccg gggcgaaaca cgcggacgat
     3001 gcccatcaac tcctggatgg cgccgtcgac ccacggccga ttgatggccc aactgaagcg
     3061 gttgatcgag aggcgcgcct cgcgattgtc gaggcagccg acgatgacgt cgacatggcg
     3121 aaaaacgccc agacccatct cgaagttgat gttgccgtgc caggccttca ccttcacgtc
     3181 ggggttcagc gccttgaccg cctcggcggc ggcgtccacc ttgcggcggc cgcgatctgt
     3241 ggtgcgaaag agcaccgatc ggctgagatt gctgtcctcg atcgtgtcga agtcggcgat
     3301 caggatgttc ccgatgccca tcagagccag gtttttgatg acctcgttgc ccaaggcccc
     3361 cgcgccgatg accagcacgg tcgcgttgcg cacgacttcc tgctgccacc agctgatgta
     3421 gccgaaggtg tgatagcggt cgctatccgg gtcagtgatg actatggttt ccggttccgt
     3481 cattatctcc tcatatcgct caggtctgga ccggtttggt cagcgtggcg aggaagtttt
     3541 gcgtcacaat cagttggccc tcgggcgcgt aggcgacgcc gatgcccacg tgagtccaga
     3601 ccgggttcag gatattggcc cggtggttcg tcaggcggcg cggctggttc atgaaggcga
     3661 aatggacttc ctgaactccg cgctcgccgt ggctcgccgg cccatagacg acgccgatgt
     3721 tctcgccgca ggcgatgtaa ctgatggcgc ggcggtcgag acgattggcg acggctacgc
     3781 cctccggtgt cgtatgggcg acgtagcgcc ggtcgagcat gtcgttggcg tgctgccggg
     3841 ccacgagggc cagttgcggg tgccattgca gcggcccacg ccccagccag ctgggcagat
     3901 gctcttcccg tgcctggttg accagccgga acgacaattg ttccagagcg ccgacgactt
     3961 cttcgctcgc cggttcaaag gcggcgatat cctcggccgt cacgtgcagg gtatggctgc
     4021 gcggcgcggc cgggagtgtc gcaatatcgg gcagctcctc aaggctgatc gtcagcaccg
     4081 gcggggccat gaccaggccg cgttgggctt cgatatcttc tgcggtgatc cgcagcgggt
     4141 cgacgtcgct catcagcagt tacgatcagt cctttagctt ccggtaaaac ggcagggtga
     4201 aatgagtccg gtcagcgttc agggtgatcg atttgcgctc gacctcaccc ccaaagtcgc
     4261 ggggccaggt tacatcgaca tcgtaggtca gcccggcgtc gagctgtacc ggcaactcgc
     4321 agatgaactc gccgtccttg ttggtcgtgc cggccacata gcgacgaata ccgcgcgaac
     4381 tgatccgcac ctgaagccca tagaggccgg ggccgttggg tcgctcttgc acgacaacct
     4441 ggagtttcgt cgccttcatg gcattgcggc cacggccgcc gcggatggct gcggatgctc
     4501 cggcggtatt ggcggtggtc gttgccttgg gcatgacgac atgggccggc cggggagccg
     4561 ccggcggatg tgtcgcgggg cgctgcgtcg ttggttgaag gggcggctca tcggccgaga
     4621 tgtcaatccg caggggcggc tgggtcgtgg gggcacttgg gccagatgac agatcggcgt
     4681 tggtgatgct gagcggcgcg ccgggtgccg gcgcggccgg atgctctatc tcatccggtg
     4741 agaccccgat ttccgggtta tattccggat agcgttcggg aatcggcatc cggtaatagg
     4801 gcggcagcgg cgcgatggtt ccgtcgggcc gcagggcgtt gagcgacggt gcgccgccct
     4861 cggcgtgggc cagcttgcgg cccagtttgt cgcgctcggt ggcgctgcgc tgcacttcct
     4921 gccggatgaa ataagcggct cgcttcagaa cctgcccggc cgggctgaag ggatcgggcg
     4981 gcgcggccgg gtccagccgg tcgagcagaa agtcggctcg ccaggcgaag gcggccacgg
     5041 ccagcgccag ccacgacggc gcgctctgct ccgactgggg cataaccccc gccgaggtgg
     5101 ccgccaggac ggcaacgtcc tctttatccg ccagcccgct gtcgcgcaac aggccataga
     5161 gcgccaggcg catgccttgt tcggattcca ctgcctgggc gaagctatgg cgagtgggtt
     5221 cgtcgagcac gttggccgcc agcaagatgg cgcgcgcccg cgattccgtc aatgactcac
     5281 cgttcgggtt gagatcgcgg ttgagccact ccagcaaacg accggcaaaa gcagccattt
     5341 gccccttgcc ggcgaaagcc tgaacttgtt tttgggcggt ttcgtgcata gcgtgctcct
     5401 tggagagacc accagccacc ggcaagtcaa tcggttgccg gtggctggta gccgttttag
     5461 attgccagtc cggtttccag gtgatgcagg ccgtcccaga cgcgcatcag ttcttcgatg
     5521 tcggagacgg actccatgtc aacttccagg gccttgcgca attccggttt gtggagattg
     5581 cggtcgtgct ggcgcagcat cggcttgacc aacaggttgt tgaagtcgcg ctcggtcttg
     5641 atcgcttcca gccagaacag gcggcgcaga cgcggcaaca ggtc
//
LOCUS       DAOMQG010000217         5672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6012_length_5672_cov_8.682393, whole genome shotgun sequence.
ACCESSION   DAOMQG010000217 DAOMQG010000000
VERSION     DAOMQG010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_5672_cov_8.682393"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..3510)
                     /locus_tag="PLC06_11450"
     CDS             complement(298..3510)
                     /locus_tag="PLC06_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRO91104.1"
                     /translation="MTDNVSMALVSPTATIGDAAAVAGFATDVDLNRVAPGASFRATW
                     TFRNSGPTTWDGRYRLIYSDAPHPETATNNRSPLGAPAAVAITDIGAPASVRPGESVS
                     LTLHFTAPQTPGVHATNWQLAGPDGGRFGPVRWLRVVVVPQARSLGYDLLGFRNSAGD
                     FNNLQPAQQFSGTWTVRNSGSRAWNGDFRVAYSDAPTADTAQLARDLMGAKPVYSLRQ
                     LSGRESVAPGESVDIRLDLIAPRETGAYAFHWQLQSDDDQVFGGVRWLRIGVVGGPDP
                     GGTRPTDGAGVAFGMNINPEAHGADVDKLAGLGWVRWVFWASRIKLSPEEAYQQKYRS
                     LIQAYAAAGIRSLIVLHQDTEWGNAPWQHGGWAAYAETFAKACGRVAAVCSEFGDRVA
                     YQIFNEQDSGPDNHSAIGIAAEDYAIVLDQAQEAIRRAHPGATVVIGGLNTGPDNAIR
                     YARKVQATLGGRLPVDALAIHPYGRYVHKILFNYGSIGKLSDSLNRFRDAFPDKPLWI
                     TEVGVANDTPIGPRHYADIGIYLREVVDEVADRYADYVPVLIWFGWTDLMRNAGILTA
                     DGQPKPNVFEAFEHMRERSKPDLESLMAEGLESLNQSKAEFLSFATTLVNYTAVPAGS
                     GFTNSWRFRNSGSTTWGDGFRLAYAPDGEGSDPLMAEHSFDLAAVAAPWPVPPGAETT
                     ITLAMTAPGRFGRAWRSRWQLRDVAGEAFGHLFAEITVIPAPTAGTGARESAMTFVRD
                     QTVPDGTPFVAGTDFHKQWAVRNTGVRHWGSGYRLVFVQGDIQMARGVPAHLVPEARP
                     GDEVILSVPMSAPPARGGQTTPYSSLWRLQDDHGAFFGDPVWAKISSTPAVPVVDGQP
                     PLQTATALGRLLNDPSAWYSQLDPRWSGVAVGHGAQRIGAWGCLVTCMAMTLTAYGAR
                     FDPLQLNERLKQEGDNGFIGANVQFVAPTVVLPGLKQGKNLRSFEDSGVPFTEWTGED
                     PLGRINNALALGQIVLAQVDTKPNNGLFDSNIEQHWVLIVQRTPNDDDYLILDPIVPA
                     GQVHDQPRSLMVKYGNRVAGQTNAINLRNAIQSTIVYHRN"
     gene            complement(3627..4418)
                     /locus_tag="PLC06_11455"
     CDS             complement(3627..4418)
                     /locus_tag="PLC06_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91105.1"
                     /translation="MSNLTTDSRHITVLNGGRVGEAETSIFTLPAGITGYADAQIDDY
                     GGQGGTFRWRPGTTLSLRARFSHDAATLRGTAGFGFWNAPYGEPSHRRPALPRAVWFF
                     FGSAPNDLPFAPDAPGRGWFAGTMDVRPSSVPALILLAPAVLLLNQFRAARKHIWPAV
                     RRQLGIHAAPIVADMTTWHEYRIEWRVDSVSFLVDGMSLLETPRSPCGPLGFVCWLDN
                     QYLVLTPRGRFRAGTLHTNTEQWMEIADLHLEAGDKTIDTLGLAP"
     gene            4510..5142
                     /locus_tag="PLC06_11460"
     CDS             4510..5142
                     /locus_tag="PLC06_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO91106.1"
                     /translation="MEINWYGMSCFRITERKQATIVTDPYAVDMGLGELKLKADVVTV
                     SHDAPGHNFTSAVSGMEHILSGPGEYEIGGVFITGIPTRSKNGRFNNIIYVYDYNGLT
                     VAHLGDIGDVPSQKQIEDLELVNVLLVPVGAGTSLNAAKAAELVSMIEPNVVIPMHYE
                     LPGLKTKLDPLEKFLKEMGATDAKTESSFKATSVDSLPEETEVVILSSKE"
     gene            5227..5529
                     /locus_tag="PLC06_11465"
     CDS             5227..5529
                     /locus_tag="PLC06_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91107.1"
                     /translation="MPAKLIMRWNVRQEMESEYFEFLVHELIPAMNKLGINDIQVWYT
                     AYGECEQKMAEGISESSDHLLRVIHTNEWHELTDRLQGYVLDFSQKVIPATRGFQI"
BASE COUNT         1073 a         1764 c         1821 g         1014 t
ORIGIN      
        1 tgcggcaggc ggtgcgggag gcgctgagcg ccagcaagca ggttcgcgcc tgggaagagg
       61 gcaaggacgg cgagggtggg ccgggcgtga cggtggcgca tatggtggaa cataaataat
      121 taggaattag gaatacacgc gctcttgcta tattcgcgcg acaggaggcg cggcgttctg
      181 ctgcctgtcg cctgtggtct gtcgtcagtt gtcgggaaac aaccaccaca agggcacgct
      241 cttcattcct aattcctaac tgatgttacg cactacgtta gtgcgtaaca tcagttccta
      301 atttctgtgg tacacgatcg tcgattgaat cgcattccgc agattgatcg cgttcgtctg
      361 cccggcgacc cgattgccgt atttgaccat caacgatcgt ggctgatcgt gcacctggcc
      421 ggccggcacg atcgggtcga ggatcagata gtcgtcgtcg ttcggcgtgc gttgcacgat
      481 gagcacccaa tgctgctcga tgttggaatc gaacaagcca ttattgggtt tggtgtccac
      541 ttgggccagg acgatctggc ccagcgccag cgcattgttg atgcggccga gcgggtcttc
      601 gccggtccac tccgtgaagg ggacgccgct gtcttcaaac gagcgcagat tcttgccctg
      661 tttcaggccc ggtaacacga ccgtcggggc gacgaactgc acgttggcgc cgatgaagcc
      721 gttgtcgcct tcctgcttca accgttcgtt gagctgcagc gggtcaaagc gcgcaccata
      781 ggcggtcagg gtcatcgcca tgcaggtcac caggcagccc caggccccaa tgcgttgcgc
      841 cccatgcccc accgctaccc ccgaccagcg cgggtccagt tgcgaatacc acgccgacgg
      901 atcattgagc agccgcccca acgccgtggc cgtctgcagt gggggttggc catcgacgac
      961 cggcaccgcg ggtgtgctgc tgatcttcgc ccaaacggga tcgccgaaga acgccccgtg
     1021 gtcatcttgc aagcgccaca gactggaata gggcgtagtt tggccgccgc gcgccggcgg
     1081 ggcgctcatc ggcacggaga ggatcacctc gtcgcccggc cgcgcttccg gcaccagatg
     1141 ggccgggacg ccgcgcgcca tttgaatgtc cccctgcacg aaaaccagcc ggtagccgct
     1201 gccccaatgc cgcacgcctg tgttgcgcac cgcccactgc ttgtgaaagt cggtgccggc
     1261 cacaaacggc gtcccgtcgg gcacggtctg gtcgcggacg aaggtcatgg ccgactcgcg
     1321 cgcgcccgtc cccgctgtcg gcgcggggat gactgtgatc tcggcgaaga gatggccaaa
     1381 ggcctcgccg gcgacgtcgc gcagctgcca gcggctgcgc caggcgcggc cgaaccggcc
     1441 gggcgcagtc atcgccagcg tgatcgtcgt ctccgctccc ggcggcaccg gccagggcgc
     1501 ggcgacggcc gccaggtcga acgaatgctc ggccatgagt gggtcgctgc cttcgccgtc
     1561 cggcgcataa gccagccgga acccgtcgcc ccaggtggtc gagccactgt tgcgaaagcg
     1621 ccagctattg gtgaagcccg aaccggcggg aacggccgtg tagttgacca gagtcgtggc
     1681 aaacgaaagg aactccgcct tcgactggtt gagcgactcc agcccctcgg ccatcaggct
     1741 ctccaggtcg ggcttgctgc gctcgcgcat gtgctcgaaa gcctcgaaca cgttgggctt
     1801 cggttgtccg tcggccgtca gaatgcccgc gttgcgcatc aggtcggtcc agccgaacca
     1861 gatgagcacc ggcacataat cagcgtaacg atccgccacc tcatcgacga cctcgcggag
     1921 atagatgccg atatcggcat aatgccgggg gccgataggg gtgtcgttgg cgacgccgac
     1981 ctcggtgatc cacaagggct tgtcggggaa ggcgtcgcga aagcggttga gcgaatcgga
     2041 cagcttgccg atagagccgt agttgaacag gattttatgg acatagcggc catagggatg
     2101 gatggccagc gcgtccaccg gcagacggcc gccgagcgtc gcctgcacct tgcgcgcgta
     2161 gcggatggcg ttatccggcc cggtgttgag accgccgatg accaccgtcg cgccggggtg
     2221 ggcgcggcgg atggcttcct gtgcctggtc gaggacgatg gcgtagtcct cggcggcgat
     2281 gccgatggcc gaatgattgt ccgggccgct gtcctgctca ttgaaaatct gataggccac
     2341 cctgtcgccg aattcggaac acacggcggc cacgcggccg caggccttgg cgaacgtctc
     2401 ggcataggcg gcccagccgc catgctgcca gggcgcgttg ccccactccg tgtcctgatg
     2461 cagcacgatg agcgagcgaa tgccggctgc ggcataggcc tggatcaggc tgcggtactt
     2521 ttgctgatag gcctcttcgg ggctgagctt gatgcgcgac gcccagaaca cccagcgcac
     2581 ccaacccagc ccggccagct tgtcgacatc cgcgccgtgg gcctcagggt tgatgttcat
     2641 gccgaacgcc acgcccgcgc cgtccgtcgg ccgcgtgccg ccggggtccg gcccgccaac
     2701 gacgccgatc cgcaaccagc gaaccccgcc gaacacctgg tcatcgtcgc tctgaagctg
     2761 ccaatggaac gcataagccc ctgtttctcg cggggcgatc aagtccagcc ggatatcgac
     2821 cgactcgccg ggggccacgc tctcgcgccc cgacagttgg cgcagcgaat agaccggttt
     2881 agcccccatg aggtcgcgcg ccaattgggc tgtgtcggcc gtgggcgcgt cggaataggc
     2941 cacgcgaaaa tcgccgttcc aggcgcgcga gccgctgttg cgcacggtcc atgtgccgct
     3001 gaactgctgg gcgggctgca ggttgttgaa atctcccgcg ctgttccgaa agccgagcag
     3061 gtcatagccg aggctccgcg cctggggcac gactacgact cgcagccagc gaaccggccc
     3121 aaagcggccg ccgtccggcc cggccagttg ccaattggtg gcgtgcaccc ccggtgtttg
     3181 gggagcagtg aaatggagcg tcagcgagac cgactcgccc ggtcgcaccg aggccggcgc
     3241 gccgatgtcg gtgatggcga cggccgccgg cgcgcccagc ggcgagcgat tgtttgtcgc
     3301 cgtctcgggg tgaggggcgt cgctatagat gagccgatag cggccatccc atgtggtcgg
     3361 gccgctgtta cgaaatgtcc acgtagcgcg aaaagatgcg cccggcgcca cgcggttgag
     3421 gtcgacgtcg gtcgcgaacc cggcgacggc ggcggcatcg ccgatagttg ctgtaggcga
     3481 aacaagagcc atggatacgt tgtcggtcat gatgttgtcc ttgtataacg gggcgaagag
     3541 gaatgattgc cgggtatttg gcaatcattt cgcccatgcg agcggtgcgg caggagcctc
     3601 gcgtcgttgc cggtgcgaat accattctaa ggggcgagac ccagtgtgtc aatcgtcttg
     3661 tctcccgctt ccagatgcag gtcggcgatc tccatccatt gttcggtgtt cgtatgtagt
     3721 gtaccggccc ggaaacggcc gcgaggggtc aataccaggt attggttatc gagccagcaa
     3781 acgaaaccca gcggcccgca gggtgatcgc ggcgtttcga ggagcgacat gccatccaca
     3841 agaaagctca cgctatcgac acgccactcg atccggtact catgccaggt ggtcatgtcc
     3901 gcgacgattg gcgcagcatg gatgccgagt tgccgtcgca cggccggcca aatgtgcttg
     3961 cgagcggcgc ggaattggtt gagtagcagg acagccgggg cgaggagaat gagcgccggc
     4021 actgacgatg gtctgacgtc catcgtgccc gcaaaccagc cgcgccccgg cgcgtcgggc
     4081 gcaaaaggca gatcgtttgg tgcagagccg aagaaaaacc agaccgctct cggcaacgcc
     4141 gggcgacggt gtgagggctc accatagggc gcattccaaa aaccaaaccc cgccgtgccc
     4201 cgcaaagttg cggcatcgtg agagaatcgc gccctcaggg agagggtcgt ccccggccgc
     4261 caacgaaatg tcccgccttg gccgccgtag tcgtcgattt gggcatcggc gtagcctgtg
     4321 atccctgccg gcagagtaaa gatagaggtc tctgcctctc ccacacggcc gccattcagc
     4381 accgtgatgt gccgggaatc agtggtcagg ttgctcatga tgctttgaac cattcgttct
     4441 atcggctata ataccttgtt tgtcggcggc cgtttccctt gtcgccgggc aacaggagca
     4501 tcgatctgca tggaaatcaa ctggtatggc atgagctgct tccgcattac agagcgcaag
     4561 caggccacga tcgtcaccga cccctacgcg gtcgatatgg gactgggcga attaaagctg
     4621 aaggccgacg tcgtcaccgt cagccacgac gcgcccggtc ataacttcac ctctgccgtc
     4681 agcggcatgg aacacatcct cagcggcccc ggcgaatatg agatcggcgg cgtgttcatc
     4741 accggcatcc caacgcggag caagaacggc cgattcaata atatcatcta cgtctacgac
     4801 tacaacggcc tgaccgtcgc ccacctcggc gacatcggcg acgtcccttc ccagaagcag
     4861 atcgaagacc tggagctcgt caacgtactt ctcgtccccg tgggcgcggg caccagcctg
     4921 aacgccgcca aggccgccga actggtgtcg atgatcgagc ccaacgtcgt catccctatg
     4981 cactatgaac tgcccggcct gaagaccaaa cttgacccgc tggagaagtt cctgaaggag
     5041 atgggtgcga ccgacgccaa gacggaatcg agcttcaagg ccaccagcgt cgactcattg
     5101 cccgaagaga cggaagtcgt catattgtcg tcaaaggaat aagctgatgc cgataatgcg
     5161 cctgggaagc cctgccctgc cggagcaaga cccacccagg atatcgacca tggcgcggag
     5221 tgagctatgc ccgcgaaact gatcatgcgg tggaacgtcc gtcaggaaat ggaatcggaa
     5281 tacttcgagt tcctcgtcca cgagctgatt ccggcgatga acaagctggg gatcaacgat
     5341 atccaggtgt ggtataccgc ctacggggag tgcgagcaga agatggccga gggcatcagc
     5401 gaatcaagcg accacctgct gcgcgtcatc cacaccaacg agtggcatga actgaccgac
     5461 cgcttgcagg gttatgtgct ggatttcagc cagaaagtga tccccgcgac gcgaggattc
     5521 cagatttagg actggccgct tgacgcagag tccgaggacg tcgcggttga ggtcgagggg
     5581 gcggattcgg tgctaatctc tttggtctcc gagcttttcg ccttgctgcc tttgccgttc
     5641 accttgctgg agcggttgtc ggtcacatag aa
//
LOCUS       DAOMQG010000218         5668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6019_length_5668_cov_7.238019, whole genome shotgun sequence.
ACCESSION   DAOMQG010000218 DAOMQG010000000
VERSION     DAOMQG010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6019_length_5668_cov_7.238019"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLC06_11470"
     CDS             <1..395
                     /locus_tag="PLC06_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009829103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HRO91108.1"
                     /translation="PRADFYHWVLVDIPADIHELPSGCDCEGVTAHGKEVGKTPYGTR
                     GINSYTSWFAGMDSMEGDYGGYDGPFPPWNDERVHHYHFTLYALDVESLGLDGAFDGA
                     DVLLAMKGHILASDNWVGTYAIYPGARP"
     gene            531..2321
                     /locus_tag="PLC06_11475"
     CDS             531..2321
                     /locus_tag="PLC06_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HRO91109.1"
                     /translation="MSEPLTQLTLIAIFGMGAQWVAWRLRLPSILLLLLSGFLLGPIL
                     GWIHPDELLGETLFPVISVSVGLILFEGGLTLKLRELPENGRVIFRLISLGALVSWVV
                     AALGAYYILGFDWPIAILTGSILIVTGPTVVGPLLRQIRPKGRAGTILKWEGILIDPV
                     GAVLALLVFEVIRLGGVDSVPIYLLLGTLRSLAVGIILGLFGAGLLVFLLKRYLIPDH
                     LQNGVTLFIIVGLFTLSDLLAPEGGLLTVIIMGIALANQSQVPIHHILEFKENLQVLL
                     LGFLFILLAGRVDFANLSHIGWPSVLFIALLIVVARPIGVFLSTWRTPLSNNERLFMI
                     WMAPRGIVAAAISSIFAFQLEALGYPEAGALAATILLVIIGTVIFYGLTAGPLARRLG
                     LAEQDPQGLLIVGAHPISRNFATELKKHHVRTLLLDNNYGNIVDSHLEGLEAFYGNAL
                     SEETLRELDLSGIGRMLALTGNDDINALAVLHFPDVFGRANVYQLPSARDGHAAPAHL
                     RGRSLFSSTATYDHMEKLMETGATIKSTALSSSFSYADYLANYEERPPIPLAVVTSDS
                     KLQLFTEDNRPTPKPGQTIISLIPAEGTSD"
     gene            2410..2973
                     /locus_tag="PLC06_11480"
     CDS             2410..2973
                     /locus_tag="PLC06_11480"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRO91110.1"
                     /translation="MSDSPRPFKTISSRIVWSCPWYRVRQDEIVTPDGQSGAYNVIEK
                     ADAVWIVPVTADGRIAMVHQFRYTIDEWCWEVPAGSVKDGQTVEVAAREELREEVGGD
                     AGEMEYIGRFYLANGICNEVGHIFLATDVRLGQPHHEAAEVIEISLKSIPESLAMAKS
                     GQISDGPSALALLICADRLTELMPNGS"
     gene            complement(2954..4831)
                     /gene="uvrC"
                     /locus_tag="PLC06_11485"
     CDS             complement(2954..4831)
                     /gene="uvrC"
                     /locus_tag="PLC06_11485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRO91111.1"
                     /translation="MKYQPSERIQELLKNLPTKPGVYLHKDESGNVLYVGKANNLRNR
                     VRSYFHTTVDSIKTQRLRRQIADIEVITTESELEALLLEMTLIKQHKPQFNVRLKDDK
                     RYPYIKVHWADPFPKVTVTRRMVRDGSRYFGPFTSAWAVQQTLDLLRKIFPYLTCDRV
                     ITGKDERACLYYDIKLCNAPCIGAVTQAEYRENIQKLMDFLDGKSDYIIRGIEAKMAE
                     AATDLQFEKAAEYRDQLKAIQRITARQKVIGSSDTDQDVIAFAREDSDACVQIFFIRH
                     GKLIGREYFMLDNTEGESDHEVLREFMTQFYDDAAYIPREVLLPSEIEEVNIIEEWLR
                     RKRNTKVTIQVPRRGKKRELVNMATENAHDTLATLRQQWAADRSKHVVAITELQDALR
                     LPAPPARIECYDISHTQGTQTVGSMVVFVQGAPQKSDYRRFNVQTVGNDDYGAMREVL
                     TRRFQRYRDTLAGELHDPAEIKARSERPTAWAILPDLLLIDGGKGQLAVGLEVLREFD
                     LEGAVPIASLAKQEEEIFVPDQPRSVMLPRRSEALYLVQRVRDEAHRFANEGHRKQRA
                     KVGVASILDSVPGVGPRRRQLLLTRFGSLEALREASLEEIAAVPGIPYEVAEGIKTHL
                     A"
     gene            complement(4895..>5668)
                     /locus_tag="PLC06_11490"
     CDS             complement(4895..>5668)
                     /locus_tag="PLC06_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRO91112.1"
                     /translation="RAALVAAALLAETWKAELHGLFVEDINLLRLSELPFAREILFAE
                     SELRRIEREELLKRLRARAAILRQEVQEIAAEYRIAGTFQVMRGSVGKELLAAVLDTD
                     LLAIGRLGHSIAHRAPLGSTARAIIAGATSAVLLVRAEVAMGPIVALYDGSDVGRRVL
                     DLAVSLTERGGDLRVLVWAEDEAVAFETRQLAAHLLETHAIHIQYQHLSGDNPQFVLQ
                     WVNRQKASLLLLGAGRANLSDHIFQMLLDEAEQNILIIR"
BASE COUNT         1210 a         1585 c         1550 g         1323 t
ORIGIN      
        1 tgccgcgggc tgatttctat cattgggtac tcgtcgacat tcccgcggac attcatgagc
       61 ttccgagcgg ctgcgattgt gaaggcgtga cagctcatgg caaagaggtc ggcaaaacgc
      121 cttacgggac gcgggggatc aatagctaca caagctggtt cgccggcatg gatagcatgg
      181 aaggcgatta cggcggctat gacggcccgt tcccgccctg gaacgatgag cgtgtccacc
      241 attatcattt cacgctctat gccctcgatg tggagtcatt aggcctggat ggcgcattcg
      301 acggcgcgga tgttcttctg gccatgaaag gccatatcct ggcctctgac aattgggtcg
      361 gcacctacgc tatatatccc ggcgcgcggc cgtagatatt tcattcaagg tagcggtcaa
      421 tataattcac atgcgccttt ttgctgctcc gcgtagacag agacggggcg aggcgcccgc
      481 tcggctatgc gaatatttag accttatgat gcggaactaa agactgccac gtgagcgaac
      541 ccctaacaca attaacgctc atcgccatct tcggcatggg cgctcaatgg gtggcctggc
      601 gattacgcct tccttccata ctcttgctac tcctctcggg ctttctgctc ggcccaattc
      661 tgggctggat acatccggat gaattgcttg gcgagacctt gttccccgtg atttctgtct
      721 ccgttggctt gatccttttc gaaggcgggc ttacgctcaa gctaagagag cttccagaaa
      781 atggtcgagt cattttcagg ctgattagcc ttggtgcgtt agtatcgtgg gtggtggccg
      841 ctttaggggc atattacatc ctgggctttg attggcccat cgcaattctg accgggtcga
      901 tactcatcgt gaccgggcct actgtcgtcg gcccgttgct gcgtcaaatc cggccaaaag
      961 gaagagcggg aacaatcctg aaatgggaag gcattctcat tgatcctgtt ggcgcagtct
     1021 tggcactgct ggtgtttgaa gtaatacgac tgggcggagt cgatagcgtg cccatctact
     1081 tgctacttgg aacgcttcgc agcctggccg tcggcattat tcttggcctg ttcggagccg
     1141 gacttctcgt ctttctattg aagcgatatc tcatccctga tcatcttcaa aatggcgtca
     1201 cgttatttat tatcgtgggg ctattcacgc tctccgatct cctggcgcct gaaggcggtc
     1261 ttctgactgt catcattatg ggcattgctt tagcgaatca gtcccaggtg cccattcatc
     1321 atatcctcga atttaaagaa aatctgcagg tcttgttgct ggggtttttg ttcatattgc
     1381 tggccgggcg agtcgatttt gccaacctga gtcatattgg ttggccatcc gtgctgttta
     1441 tagccctatt aatcgtcgtg gcgcggccga tcggcgtatt cctctcgaca tggagaacgc
     1501 ctctatccaa caatgagcgt ctgttcatga tttggatggc accacgaggc atcgttgccg
     1561 cagccatctc ttccattttc gccttccagt tagaggcgtt gggctatccc gaagccggtg
     1621 cgctggccgc tacgatcctg cttgtcatca ttggcactgt tattttttat ggcttgacag
     1681 ccgggccgct ggcgcgacgg ctcggcctgg cggaacaaga cccgcaaggg ctgctcatcg
     1741 ttggcgcaca tcctatttcc cggaacttcg ccactgaatt aaagaagcat catgtgcgca
     1801 cgcttctcct ggataataat tatggcaata tcgttgacag ccacctcgag ggcctggaag
     1861 ccttttacgg caacgcactg tcggaggaaa ctctgagaga actcgatctg agtggcattg
     1921 ggcggatgct ggccctcaca ggcaacgacg atatcaatgc tctggctgtg ctccacttcc
     1981 ccgatgtatt cgggcgagcg aatgtttatc aactaccctc ggcgcgggat ggacacgccg
     2041 cccctgctca tctacgtggc cggtcgctgt ttagctcaac ggccacttat gatcatatgg
     2101 agaagctaat ggagacagga gcgacgatca aatcaacagc tctgagttca tcgtttagct
     2161 atgccgacta cctggccaat tatgaagagc gcccgccaat tccccttgcc gtggtaacga
     2221 gcgattcaaa actgcagcta ttcacagaag acaatcgtcc cacacccaaa ccgggccaga
     2281 cgatcattag cttgattcct gcagagggca ccagtgacta aagttaaaac ctgggatcgc
     2341 atccgttggg aagatatggc cgccactctg gaaaacatga aacacccctg ataactaaag
     2401 gctattgaaa tgagcgacag cccgcgacca ttcaaaacca tatccagtcg cattgtctgg
     2461 tcctgcccct ggtatcgagt caggcaagac gagatcgtca ctcccgacgg ccaatcgggc
     2521 gcctacaacg tcattgagaa agccgacgcc gtgtggatcg tgcccgtcac ggccgatggc
     2581 cggatcgcga tggtgcatca gtttcgctac accattgacg aatggtgttg ggaagtgccc
     2641 gcggggagcg tcaaagacgg ccagacggtt gaagttgcgg ctcgcgaaga gctgcgagaa
     2701 gaggttggcg gggatgccgg tgagatggag tatatcggcc gattctatct ggcaaacggc
     2761 atctgcaatg aggtcgggca catcttcctg gccaccgatg tacggcttgg acaaccccat
     2821 cacgaggcgg ccgaagtgat agagatcagt ctgaaatcca ttcctgaatc gttggcaatg
     2881 gcgaagtctg gtcaaatcag cgacggaccg agcgcactcg cgcttctgat atgcgccgat
     2941 cggctgacag aactcatgcc aaatgggtct tgatgccctc ggcgacctca taggggatac
     3001 ccggcacagc ggcaatctcc tccagcgagg cctcccttag cgcctccaga gagccgaacc
     3061 gggtcagaag aagctgacgg cgacgtggcc ctacgccggg gacgctatcc aggatagaag
     3121 ccacccccac cttggctcgc tgcttgcgat ggccttcatt cgcgaagcga tgcgcctcat
     3181 ctctcacccg ttggaccagg tacaacgcct cggatcggcg aggcagcatg acggatcgcg
     3241 gctgatcggg cacgaagatc tcttcttctt gcttagcaag agaggcgatg gggaccgcac
     3301 cttccagatc gaactcgcgc aaaacctcca gaccgaccgc caattgcccc tttccgccgt
     3361 cgatgagcaa aagatcaggg agaatggccc aggcggtcgg ccgctctgag cgcgccttaa
     3421 tctccgccgg atcatgtagc tcaccggcca gcgtatcccg gtagcgctgg aagcgccgtg
     3481 tcaatacttc ccgcatggct ccgtaatcat cattaccgac tgtctgcacg ttgaatcgcc
     3541 gatagtcgct tttctgtgga gcaccctgga caaaaacgac catcgagccg accgtttgtg
     3601 tcccttgcgt atggctgatg tcatagcact caattcgggc gggtggtgcg ggcaatcgca
     3661 atgcgtcctg cagctcagtg atcgccacaa catgcttcga ccggtcggcc gcccactgct
     3721 ggcggagcgt ggccagcgtg tcgtgggcgt tctcggtcgc catattgaca agctcgcgct
     3781 tcttgccccg acgtggtacc tgaatagtga ccttggtatt ccgcttgcgt cgcagccatt
     3841 cctcgatgat attgacttct tctatctcgc tcggcagcaa gacttctcgc gggatgtagg
     3901 ccgcatcatc gtagaactgg gtcatgaact cccgcaggac ttcgtggtcg ctctcaccct
     3961 cagtgttatc gagcatgaaa tattcacgac cgataagttt gccatggcgg atgaagaaga
     4021 tctgcacgca tgcgtcgcta tcctcgcgag cgaaagcgat aacgtcctga tcggtgtcgc
     4081 tggagccaat cactttctgg cgagcggtga tgcgttgaat cgcctttaac tgatcgcgat
     4141 attcggccgc cttctcgaat tgcagatccg tcgcggcctc ggccattttc gcttcgatgc
     4201 cgcggatgat gtaatccgat ttgccatcca aaaaatccat gagcttctgg atattttcgc
     4261 gatattccgc ctgggtgact gcgccgatac aaggggcatt gcaaagcttg atgtcgtaat
     4321 agagacaggc gcgttcgtcc ttgccggtga taactcgatc acaagtgaga taagggaata
     4381 tctttcgcag caagtccagc gtttgttgca ccgcccaagc ggaagtgaac gggccaaaat
     4441 agcgcgagcc atcgcgcacc atgcgacggg tgacggttac cttggggaag gggtcagccc
     4501 aatgaacctt gatatatggg tagcgcttgt cgtccttgag ccgcacattg aactgtggct
     4561 tgtgctgctt gatgagcgtc atctccagca acagcgcttc cagctccgat tcagtggtaa
     4621 tcacctcgat atcggcaatc tgccggcgca ggcgctgcgt cttgatggaa tcaacggtgg
     4681 tgtggaaata ggaacgaacg cggttgcgca ggttgttggc cttgccaaca tacagcacat
     4741 taccgctttc atccttgtgc agataaacgc ccggtttcgt cggcaggttc tttagcagtt
     4801 cctgaattcg ttcgcttggc tggtacttca tggcgtgcaa aataacgtgc ctgattcatg
     4861 ggcacgtgtt tgattctagc acaagttttc tgttttacct gattatgagg atgttttgct
     4921 cagcctcatc gaggagcatc tgaaaaatat gatcagagag attggctctg cccgcaccaa
     4981 gcaagaggag gctggccttc tgcctgttta cccattgcag cacgaactgc gggttgtcac
     5041 ctgagagatg ctgatactgg atatggatgg catgagtttc cagcaagtga gcagctaatt
     5101 ggcgagtttc aaatgcaacg gcctcatcct ccgcccaaac gagtacacgc aagtcgcctc
     5161 ctcgttcagt caatgatacg gccagatcga gaacgcgccg tccgacgtcc gaaccatcat
     5221 acagagcgac aattggcccc atagccactt ctgccctgac cagcaacaca gccgacgtag
     5281 ctccggcaat gatagcccgc gcggttgaac caagcggagc acgatgcgcg atggagtgac
     5341 caagtcgccc gatggccagc agatcggtat ccagcacagc ggccagcagc tccttaccga
     5401 cggagcctcg catcacctgg aaggtaccgg caatccggta ttcggcagct atttcctgga
     5461 cttcctgtct gagaatagca gcgcgggcgc gtaatcgctt caggagctct tctctttcta
     5521 tccggcgtaa ttcagactca gcaaacagta tctcgcgggc gaagggcaat tctgaaagtc
     5581 gcagcaagtt gatgtcctcg acaaacaagc cgtgaagctc agccttccat gtctccgcca
     5641 gcagcgcggc ggccaccaga gccgccct
//
LOCUS       DAOMQG010000219         5663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6026_length_5663_cov_10.339693, whole genome shotgun sequence.
ACCESSION   DAOMQG010000219 DAOMQG010000000
VERSION     DAOMQG010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6026_length_5663_cov_10.339693"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLC06_11495"
     CDS             <1..1257
                     /locus_tag="PLC06_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91113.1"
                     /translation="GSASPDRVVRVRLYKYMARVPLAATIVLLVFVLVNRAPPLFGLS
                     KEVVLGFAVVATVMVAEWIIHQYKKTFERAFQLDDDPDVRRIQALSERLLTTSDLEQF
                     LESVLAAACEVVRTPTAFVASLTPGGPRLEAVVGPLDGMASVELQKLSLPVRGNGSGD
                     DNDDDPTDDFFVWGEYWIQPLYNNRSDLLLGILGMKARAPEPDLTADELDILSRLRLQ
                     AAAALEDRILQQEVFAAVEGILPEIIALQERRSAATFGGLPVLTSTANRVHEELLQDP
                     EFSNMVREALTHYWGGPKLTESPLLQLEVVQRGMEEHDNNPVSALRAILAEAIEQQKP
                     DGQRSLTTTEWILYNILDLKFVQGRRVRDVARRLAMSESDLYRKQRVAIENVARTVAT
                     MERATLDDHEAPLELSPITDDSLVPN"
     gene            1286..2500
                     /gene="lysA"
                     /locus_tag="PLC06_11500"
     CDS             1286..2500
                     /gene="lysA"
                     /locus_tag="PLC06_11500"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRO91114.1"
                     /translation="MHCEQVPLTVIVAAADTPVYVYSRAEILVRARAMLDTANRVTGD
                     RALICYAVKANGNPTIVRMLRDAGLGADVTSGGELFLARHAEVAPDKIIFSGVGKKSA
                     EIAEALTAGIRAIHVESEMELTAVAAEAERLGVAAPVGVRVNPNISAETHPYISTGLH
                     GHKFGVPRERAIGILRAAAAHPHLRPVGLAAHIGSQITDLDPFSQAVRFLIALADEMK
                     AEGIALEYIDAGGGLGIDYQGSAPGAADWVEVIGKLIADAGYGLVLEPGRSIVGRAGA
                     LLTRVVYTKEQGHKQFIIVDAGMTDLIRPTLYQAHHPIVPIMEPGDEYQIVDIVGPIC
                     ETGDFLARDRSLPATGQGDLLAVLHAGAYGFAMSSNYNGHLRPAEVLVDGDTFRVIRR
                     RQGYEALITGTE"
     gene            complement(2526..2846)
                     /locus_tag="PLC06_11505"
     CDS             complement(2526..2846)
                     /locus_tag="PLC06_11505"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HRO91115.1"
                     /translation="MNNKIRPVNVTADREKRVLTINWSDSRECAYSFAGLRAICPCVQ
                     CQGGHDLMGRPADKLLLGKSVNEELNLNSINAVGSYAIHFVWNDGHDSGIYTWQYLYD
                     ACLP"
     gene            complement(2862..5537)
                     /locus_tag="PLC06_11510"
     CDS             complement(2862..5537)
                     /locus_tag="PLC06_11510"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRO91116.1"
                     /translation="MTEMPKAYDFHGTEERLYQWWEENGWFKPEARPADAKPFVISIP
                     PPNVTGELHMGHAMFVALEDLMIRRARMQGRAALWVPGVDHAGIATQLQVEKLLRSEG
                     TSRQEVGREEFLRRTWAWKEKYGSHITRQLRRLGASCDWDRERFTLDEGLSAAVREAF
                     VRLHRMGLIYQDEYLVNWSPGLQTAVSDLEVEYHEEQGLLYYFKYPIQGGGYLPVATT
                     RPETILGDTAVAVHPGDSRFTDYIGRTALVPILNREIPIIADDYVEMAFGTGALKVTP
                     GHDPNDFEIGRRHKLDTISAMNKDATMSAAAGPYAGLDRFEARKRLWSDMEAAGLTIK
                     AEPHTLNVPRSQRGGEIIEPLISKQWFVNAKPMADMALEAVHKGRITIVPDHFEKVWD
                     NWLTNIHPWCISRQLWWGHRIPVWTCLACGHQTVATHDPTSCEACGNADIIQDPDVLD
                     TWFSSGLWPFSTLGWPEQTPDLARFYPTDVMETGYDILFFWVARMVMAATLFTNDIPF
                     HTVYLHGLVRDGNGRKMSKTTGNVTDPIDVIQDFGADALRFTLLTSGSPGADLNLSLD
                     RVESNRNFGNKLWNIARFVIRNFDRAGKEDRPADLSVALSTADRWILTRLGETIATVD
                     RLMDGYLFGEAGRQIYDFMWNDFADWYVEAAKIQLNVSGESAWTTLHVLGTVLDHTLR
                     LLHPYIPFVSEETWQQLRAAALESGVGIGTQDEWAEALIIADWPQPTLNDPTASADFE
                     LMRDLIRRIRNARAEQGVEPGRYIPAVIAAGNRQGLLESQRPILAALARLDDDALVIA
                     AGATPPAQAITLALGPITCYLPLAGMIDLAAERTRLEKEDGELTKQIDRLSALLNSPF
                     AQKAPAAVVEKEREKLAALETGQAEIRERLRSLDA"
BASE COUNT         1070 a         1667 c         1734 g         1192 t
ORIGIN      
        1 ggctcagctt ctcccgaccg tgtggttcgc gtacgcctgt acaaatacat ggcgcgtgtg
       61 ccactggcgg ccactatcgt actgttggtg ttcgtcctcg taaaccgggc cccacccctg
      121 ttcggcctca gcaaagaagt tgtcctgggt tttgccgttg tcgccacggt catggtcgcc
      181 gagtggatca ttcatcagta taaaaagaca tttgagcgtg cgttccagct tgatgatgat
      241 cctgatgtgc gccgcattca ggcgttgtcc gagcgtctgc tcacgacaag cgatctggaa
      301 cagttcctgg agagcgtcct ggccgcagcc tgcgaggtgg ttcgtactcc gaccgccttc
      361 gtcgccagcc taacccccgg tgggcctcga ctggaggctg tggtcggccc gcttgacggg
      421 atggccagcg tggaattgca gaagctgtcc cttccggttc gcgggaacgg gagcggtgac
      481 gacaatgacg atgatcccac cgacgacttt tttgtctggg gcgaatactg gattcaaccg
      541 ttgtataaca atcggagcga tctgttgctt ggcatcctgg ggatgaaagc acgcgctcct
      601 gagccggatc tgacggccga cgaactggac atcctgtcac gtttgcggtt gcaagcagca
      661 gctgctctgg aagaccgaat cctgcaacag gaggtctttg ctgctgtgga gggcatcttg
      721 cccgagatta tcgccttgca ggagcgacgc agcgcggcga ccttcggtgg tttgcctgtg
      781 ttgacttcca cggccaatag ggttcatgaa gagcttctcc aggaccccga attcagcaac
      841 atggttcgcg aggcgctgac tcactattgg ggtgggccga agttgaccga aagcccgctg
      901 ctccagcttg aggtcgtcca gcgaggcatg gaagaacatg acaataatcc ggtcagcgct
      961 ctgcgcgcca ttctggccga ggcgattgag cagcaaaagc ccgacggcca gcggagcttg
     1021 accacgactg aatggattct ctacaacatc ctggatttga aatttgtcca gggtcgccgt
     1081 gtgcgggatg ttgcccgccg cctggcgatg agcgaatcgg acttatatcg caaacagcgc
     1141 gtcgccatcg aaaatgtggc ccggacggtc gccaccatgg agcgcgccac ccttgacgac
     1201 cacgaggccc cccttgagct atcccccatc accgacgact cccttgtccc caattgacag
     1261 tccattcgta tatcgtgccg gtgagttgca ttgcgagcaa gtgccgttaa ccgtcatcgt
     1321 cgcggccgcg gatacgccgg tttatgtgta tagccgggct gaaatcctcg tgcgggcgcg
     1381 ggccatgctc gatacggcta atcgggtgac cggcgaccgg gccttaatct gctatgctgt
     1441 gaaggccaat ggcaatccga ctattgtacg aatgctgcgg gatgcggggc tgggggctga
     1501 tgtgaccagt gggggagagt tgttcctggc tcgccatgcg gaagttgcgc cggacaagat
     1561 cattttctcc ggtgtcggca agaaatcggc cgaaattgcc gaggcgctga ccgccggcat
     1621 tcgggccatt catgtggaat cagagatgga gctgacggca gtggcggccg aagccgaacg
     1681 gcttggtgtt gcggcacctg tcggcgtgcg ggtcaacccc aatatcagtg ccgaaaccca
     1741 cccctacatc agcaccggtt tgcacggcca caagttcggc gttcctcgcg agcgggcaat
     1801 cggcattctc cgcgcggccg ccgctcatcc ccatctgcgg ccggtcggcc tggccgccca
     1861 tatcggctcg caaataacgg atctcgaccc attcagtcag gcggttcggt tcctgatcgc
     1921 gctggcggat gagatgaaag ccgagggcat tgcccttgaa tatatcgacg ccggcggggg
     1981 attagggatc gattatcagg ggagtgcgcc gggcgccgcg gattgggtcg aggtcatcgg
     2041 caaactgata gcggatgccg gttacgggct tgtgctggag ccggggcgct cgattgtcgg
     2101 acgcgcgggc gcgttgctga cgcgggttgt ctataccaag gaacaagggc acaaacagtt
     2161 catcatcgtc gatgcgggca tgacggacct gattcgtccc acgttatatc aggcacatca
     2221 tcccatcgtg ccgatcatgg agccgggtga tgaataccag attgtggata tcgtaggccc
     2281 gatctgtgag accggcgact tcctggcgcg ggatcgctca ttgccggcca ccggtcaggg
     2341 ggatttgctg gctgtgttgc atgccggggc ttatggcttt gccatgagtt ccaattataa
     2401 cgggcacctg cgcccggccg aggtgctggt agacggcgat acgttccgcg tgatacgccg
     2461 gcggcagggc tatgaagcgt tgataaccgg tacggaataa ccgctcgagt tagtgttatt
     2521 cggcgctacg gcaggcaggc atcatacaga tattgccacg tatagatacc tgagtcgtgg
     2581 ccgtcgttcc atacaaaatg gatcgcataa cttccaacgg cgttgatcga gttgagattc
     2641 agttcctcat taactgattt tcccagcagc aatttatcag cagggcggcc catcagatca
     2701 tggccgccct ggcattgcac gcaggggcaa atggctcgca gccccgcgaa ggagtaggca
     2761 cactctcgac tatcgctcca attgatggtc agaacgcgct tctcgcggtc ggccgtcacg
     2821 tttacaggtc gaatcttgtt gttcatatcg tttcagtcaa atcatgcgtc cagcgaacgc
     2881 agtcgttcgc gtatctcggc ttgccccgtc tcgagagcgg ccagcttttc ccgttctttc
     2941 tccaccaccg cagccggcgc cttttgggca aacgggctat tgagcaacgc agaaagccgg
     3001 tcgatctgtt tggtaagctc cccatcttct ttttcgagcc gggttcgttc ggccgccaga
     3061 tcgatcatcc cggccagcgg caaatagcag gttatcgggc cgagagccaa tgtgatcgcc
     3121 tgtgccggtg gtgttgcccc ggcggcgatg accagcgcgt cgtcgtccag tcgcgccaga
     3181 gcagccagga ttggcctctg cgactccagc aaaccttgtc gatttccggc ggcaatgaca
     3241 gcggggatat agcgcccggg ttcgacccct tgctccgccc gcgcgttgcg gattcggcgg
     3301 attaagtcgc gcatcagctc gaagtcggcc gatgctgtcg ggtcgttcag cgttggctgg
     3361 ggccaatcgg cgatgatcaa tgcctctgcc cactcatcct gtgtgccgat gcccaccccc
     3421 gattccagtg cggcggcacg caattgctgc caggtctcct ctgaaacaaa tgggatgtag
     3481 ggatggagca ggcgcaacgt gtggtccagc accgtcccaa ggacgtggag agttgtccac
     3541 gcactctctc cgctgacgtt gagctgaatt ttggcggcct ccacgtacca atcggcgaag
     3601 tcgttccaca tgaaatcata gatctgccgg cccgcctcgc caaacaggta gccatccatc
     3661 agacgatcaa ccgtggcgat cgtctcgccc agccttgtca gaatccagcg gtcggccgtg
     3721 ctgagcgcga ccgacaagtc ggccgggcga tcttctttgc ctgctcggtc gaagttacga
     3781 atgacgaagc gggcgatgtt ccagagcttg ttgccgaagt tacggttgct ctccacgcga
     3841 tcaagcgaca ggttcagatc cgcgccgggg gaaccgctgg tcaggagcgt aaagcgcagc
     3901 gcgtcggcgc cgaaatcctg gatcacgtcg atcggatcgg tgacgttgcc cgtggtcttg
     3961 ctcatcttgc ggccgttgcc gtcgcgcacc aatccatgca gatagacagt gtggaacggg
     4021 atatcattgg tgaagagcgt cgccgccatc accatgcgcg ccacccagaa gaacaggata
     4081 tcgtaccccg tctccatcac gtcggttggg tagaaacggg ccaggtccgg cgtttgttcg
     4141 ggccagccga gcgtggagaa cggccacagc ccactgctga accacgtatc gagcacgtcg
     4201 gggtcttgaa tgatgtcggc attgccacac gcttcgcagg acgtcgggtc gtgggttgca
     4261 accgtctgat gaccgcaggc taagcatgtc cacaccggaa tgcggtggcc ccaccacagc
     4321 tggcggctga tgcaccacgg gtgaatgttg gtcagccagt tatcccaaac cttctcgaag
     4381 tggtcgggca cgatggtaat gcgccccttg tggacggcct caagcgccat atcggccatt
     4441 ggtttggcgt tcacaaacca ttgcttgctg atcagcggct cgatgatttc gccgccgcgt
     4501 tgacttcggg gcacgttgag ggtatgcggt tcagccttga tggtgagccc cgcagcttcc
     4561 atgtcgctcc acaggcgctt gcgggcttcg aatcgatcca gtcccgcata aggcccggcg
     4621 gccgcggaca tcgtcgcatc cttgttcatg gccgagatag tatccagctt gtgccgccgg
     4681 ccgatctcga agtcattcgg atcgtggccg ggggtcacct tgagcgcgcc ggtgccgaag
     4741 gccatctcca cgtagtcatc ggctatgatg ggaatctcac ggttcaggat cggcaccagc
     4801 gccgtgcggc cgatgtaatc ggtgaagcgg ctgtcgccgg ggtggacggc cacggccgtg
     4861 tcgcccagaa tggtctccgg gcgcgtggtg gccacgggca gatagccgcc gccctggatg
     4921 gggtatttga aataatagag aaggccttgt tcctcgtggt attcgacctc cagatcggac
     4981 acggccgttt gcaggcccgg cgaccagttg accagatatt catcctgata gatcagtccc
     5041 atacgatgga ggcgcacgaa cgcctcgcgc acggcggccg atagaccctc gtcaagggtg
     5101 aaacgctcgc gatcccaatc gcacgacgcg cccagccggc gcagctggcg ggtaatgtga
     5161 ctgccgtatt tttccttcca ggcccaggtg cgacgcagga actcctcacg gccgacttcc
     5221 tgacgtgatg tgccttccga gcgcagcagt ttctccacct gcaactgggt ggcgatgccg
     5281 gcgtggtcga cgccgggaac ccacaacgcg gcccggccct gcatgcgcgc ccggcgaatc
     5341 attagatctt ccagagcgac aaacatggca tggcccatgt gaagttcgcc cgtcacgttg
     5401 ggcggcggga tggagatgac gaacggcttg gcgtcggccg ggcgcgcctc cggcttgaac
     5461 cagccatttt cttcccacca ttggtatagc cgctcttctg tgccatgaaa gtcgtaggct
     5521 ttgggcatct cggtcattgt tgattcctct catgcggtcg gacaaaccgt ttgtttgccc
     5581 gacaaaataa aaacgctctc gtccaatcaa ggacgagagc gaattgctca cgcggtacca
     5641 ccttgcttcc gatcccatgg gaa
//
LOCUS       DAOMQG010000220         5625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6070_length_5625_cov_7.924237, whole genome shotgun sequence.
ACCESSION   DAOMQG010000220 DAOMQG010000000
VERSION     DAOMQG010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6070_length_5625_cov_7.924237"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5298)
                     /locus_tag="PLC06_11515"
     CDS             complement(<1..5298)
                     /locus_tag="PLC06_11515"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRO91117.1"
                     /translation="MNLRKQFTILFILMAALLALAACRRGRTETVPTVAPTAAAVEAE
                     VAEPTDAPSTPAEPEPTAATVVRPVPVEDIDWPPQVVTGDPLPGQEVAANAPITVRFD
                     QPMDQPSVEAAFAIEPAVAGAMSWPAPDTAVFTPAGALEPGQAYHVRIADSAASMNGQ
                     TLQEAVEFAFQATGPLRVTQTIPADGARSVQTDAVVTVVFDKPVVPLVSSGEQAGLPQ
                     PLAFDPPIEGAGEWTSTSIYRFTPEPAFAGATTYIVSVNPALTDVTGGLIDAPPTWQF
                     TTVSPEVVTVEPANDAARVTPTTPITVTFNMPMDTAATEAAINLSEAAGATTAGATAV
                     EFDWQDGDRVVVLRPAEPLALETEYTLAVASSATSANGAAAMARVHTSRFTTIPFPAV
                     ISTSPIDGGMADVWGNGFSVEFASPMNDATIEDQFVISPAPEKADYFINEWEGSYSVY
                     VQFTLIPNTEYTVTVPATAADPYGNTLGEDYTFTFTSAPTPPLASFNLPFQQAQLSTS
                     FPTDVAIMYRSVSALDVSLYPVEAGSELVFNPYRMNERQPDGEPLFRSTIPTEVVGDE
                     FGVMSVSLAYGGVLPTGIYELRVTAPEVGDDANWWQNRNVMLIVADTNLVVKEMFGEV
                     NIWATNLATGQPTAGLNLTLYRRNGEATGTAVTDADGFARFEYEPLENYLDGVTVISN
                     EPGSDGFGMASTNWTGNVSPWQMGINNDTAAEQDIYAYIYTDRPIYRPGDTVFYKGIV
                     RDTHFGRYTLPDIAQMEITINPGFFMGEESFSDSFDVTLDADGVFHGEYLLPDDLPLG
                     DYTFNITGDFWLSSRSFTVAQYRAPEFTVTVTPEQAELLRGQATQATVNATYLFGGSA
                     AGLPVSWTINQSEFLPTFEAWPAYAFGDTGDFNYVKTTTFMGDSSYLTDGSGETDAAG
                     NAVIPLPADLLADIEEGSRTVKVEATVGGLGEFPVSGRTELTFHAAEVYAGLRAGSYL
                     VNSGDAASVDIVTVDWTGAPVANQPVEVAFYRRDWESERTIGFGMYTTQWTPVDTEVE
                     RQTATTDSAGKATVEFTPDSGGTFLAVATVTDDGRQQMSSLSIWSVDAAYFGWRTDPN
                     KRTLDLTPDKTTYRAGETARVLVQSPFAQPVNAWLTIERGTLIEQRVITVTGSEVVEI
                     PISAEFAPNVHVTIAAVKPVNDDPDFPYADIRLGFVELTVPPDQFDLNVTVTPGAAEY
                     APGDTAAFEVAVTDQSGAPVQAEVSLALVDLAVLMLKEDNAPHILDAFYAPQPLRSNT
                     GSGLLITGEGLPIEEPLPNGGMGGGGGGDVALESARLEGEDDDVRRDFKDTAYWEAKV
                     MTDANGRASVEVPLPDNVTTWRMHSKAATTETEVGQGEADILARLPLIIRPVTPRFFT
                     VGDKLSLGANVNNNTDAAIEATVSLEATGLTIDGDVEQTITVPANGRVLVSWPVTVED
                     LPAADLTFRVAGGDYRDASKPTMGVGDDALLPIYRYDGRDFVATAGELAVAGRRVEAL
                     ILPQGVDTRHGEALVRLQPSLAAAIIESFDIVNDPLYPFLECAGSLADRLLHNVSVEA
                     AIRELALDNDTLATALAAQNAADVPKLVALQKADGGWGWCFSADSDPWISAQAILALT
                     RAQELGYDVSTIALNNDADYLGRQLQDVDQLGNAGEANRQAFFLYVLSETGEDVAGDA
                     DELMAEHRALLDPYAKALLAMAYENAGATGENQAALLADLNDEVIMSATGAHWEDAEP
                     DFLNLSSNIRGTAMVIQALAQLQPDSPLLPPAVRWLMVAR"
BASE COUNT          875 a         1690 c         2049 g         1011 t
ORIGIN      
        1 gcgggcgacc atgagccagc gcaccgccgg cggcagcagc gggctgtccg gctgcagctg
       61 cgccagcgcc tggatgacca tcgccgtgcc gcggatgttg ctgctcaggt tcaggaagtc
      121 cggctcggcg tcttcccaat gggcgccggt ggcgctcatg atgacctcgt cgttgaggtc
      181 ggccagcagc gcggcctgat tctcgcccgt cgcgccggcg ttctcgtagg ccatcgccag
      241 cagcgccttg gcatacgggt ccagcagggc gcggtgttcg gccatgagtt cgtcggcatc
      301 cccggctaca tcctcgcccg tttcggacag gacgtagagg aagaacgcct gccggttggc
      361 ttcgccggcg ttgcccagtt ggtcaacgtc ctgtaactgg cggccgaggt aatccgcgtc
      421 gttgttgagc gctattgtgc tcacatcgta gcccaactcc tgggcgcggg tcagggccag
      481 aatcgcctgg gcgctgatcc acggatcgct gtcggcgctg aagcaccagc cccagccgcc
      541 gtccgccttc tgcagggcga cgagcttggg cacgtcggcc gcgttttggg cggccagcgc
      601 cgtcgccagg gtgtcgttgt cgagcgccaa ctcgcggatg gccgcctcga ccgatacgtt
      661 atggagcagc cggtcggcca gcgagccggc gcattccagg aacgggtaca gagggtcgtt
      721 gacgatgtcg aacgactcga tgatggccgc ggccagcgac ggctggagcc tgaccagcgc
      781 ctcgccgtgg cgcgtgtcca cgccctgcgg caggatcagc gcctcgacgc gccgcccggc
      841 cacggccagc tcgccggcgg tggccacgaa gtcgcggccg tcatagcgat agatgggcag
      901 cagcgcgtcg tcgcccacgc ccatggtggg cttgctggcg tcgcgatagt cgccgcccgc
      961 gacacggaag gtcaggtcgg cggcgggcaa atcttccact gtgaccggcc agctgaccag
     1021 cacgcggccg ttggccggaa cggtgatcgt ctgctcgacg tcgccgtcga tggtcaggcc
     1081 tgtcgcttcc agactgacgg tggcctcgat ggcggcgtcg gtgttgttgt tcacgttcgc
     1141 gcccagcgac aacttgtcgc cgacggtgaa gaagcgcggc gtcaccgggc ggatgatgag
     1201 cggcaggcgg gccaggatgt cggcctcgcc ctgcccgacc tcggtctccg tcgtggccgc
     1261 cttgctgtgc atgcgccagg tggtgacatt atccggcagc ggcacctcga ccgaggcgcg
     1321 gccgttggcg tcggtcatca ccttggcttc ccagtaggcg gtgtccttga agtcgcggcg
     1381 cacgtcgtcg tcttcgccct ccagccgcgc cgattccagc gccacatcgc cgccaccacc
     1441 cccgcccatg ccgccgttgg gcagaggctc ttcgatgggc agcccctcgc cggtgatcag
     1501 cagtccggat ccggtgttgc tgcgcaacgg ctggggcgcg tagaacgcgt ccaggatatg
     1561 gggggcgttg tcttccttca gcatcagcac ggccagatcg accagcgcca gcgagacttc
     1621 ggcctgaacc ggcgcgcccg actggtcggt gacggccacc tcgaaagcgg ccgtgtcgcc
     1681 gggagcatat tcggccgcgc cgggcgtaac ggtcacgttc agatcgaact gatccggcgg
     1741 gacggttagc tcgacgaaac cgaggcggat gtcggcgtag gggaagtcgg ggtcgtcatt
     1801 gaccggcttc acggcggcga tggtcacgtg gacattgggc gcgaactcgg ccgagatggg
     1861 tatttccact acctcgctgc cggtgacggt gatgacgcgc tgctcgatga gggtgccgcg
     1921 ctcgatggtc agccaggcgt tgaccggctg ggcgaaggga gattgtacca ggacgcgcgc
     1981 cgtctcgccc gcccgatagg tggtcttgtc cggcgtcagg tcgagggtgc gtttattggg
     2041 gtcggtgcgc cagccgaagt aggcggcgtc gaccgaccag atggacaggc tgctcatctg
     2101 ttgccggccg tcgtcggtga cggtggctac ggccaggaag gtgccgcccg aatcgggcgt
     2161 gaactcgacg gtcgccttgc cggccgagtc ggtggtggcg gtttggcgct cgacctcggt
     2221 atcgaccggc gtccattggg tggtgtacat gccgaacccg atcgtgcgct cgctctccca
     2281 gtcgcggcgg tagaaggcga cttcgaccgg ctggttggcg accggcgcgc ccgtccaatc
     2341 gacggtgacg atatcgacgc tcgccgcgtc gccgctgttg acgagatagc tgccggcgcg
     2401 caggccggcg tagacctcgg cggcgtggaa ggtcagctcg gtccggccgc tgaccgggaa
     2461 ctcgcccagc ccgccgacgg tggcttccac cttgaccgtg cggctgcctt cctcaatatc
     2521 cgccaacagg tcggccggca gcgggatgac cgcgttgccg gcggcgtcgg tctcgccgct
     2581 gccgtcagtc agataggagc tgtcccccat gaacgtcgtg gtcttgacgt agttaaaatc
     2641 gcccgtgtcg ccaaaggcgt aggcgggcca ggcctcgaag gtcggcagga actccgactg
     2701 gttgatggtc cagctgaccg gcagcccggc ggccgagccg ccgaacaggt aggtggcgtt
     2761 gacggtggcc tgggtcgcct gcccgcgcag gagttccgcc tgctccgggg tgacggtcac
     2821 cgtgaactcc ggcgcgcgat attgggccac ggtgaacgag cggctcgata gccagaagtc
     2881 gccggtgatg ttgaacgtgt agtcgcccag cggcaggtcg tcgggcagaa gatattcgcc
     2941 atggaacacg ccgtccgcgt cgagggtgac gtcgaagctg tcgctgaagg actcctcgcc
     3001 catgaagaag ccgggattga tcgtgatctc catttgagcg atgtcgggca gggtgtagcg
     3061 gccgaagtgg gtgtcgcgga cgatgccctt atagaagacg gtatcgccgg gccgatagat
     3121 gggccggtcg gtgtagatgt aggcgtagat gtcctgctcg gctgcggtgt cgttgttgat
     3181 ccccatctgc cacgggctga cgttgcctgt ccagttggtc gaggccatgc cgaagccgtc
     3241 gctgccgggt tcgttgctga tgacggtcac gccgtcgaga tagttttcca gcggctcgta
     3301 ttcgaagcgg gcgaagccgt cggcatcggt gacggccgtt cccgtcgcct cgccgttgcg
     3361 acgatagagc gtcaggttaa gcccggcggt gggttggccg gtggccagat tggtggccca
     3421 gatgttgacc tcgccgaaca tctccttaac gaccagattg gtgtcggcca cgatcagcat
     3481 gacgttgcgg ttttgccacc agttggcgtc gtcgcccact tcgggcgcgg tgacgcgcag
     3541 ttcgtagatg ccggtcggca gcacgccgcc ataggccagc gacaccgaca tcacgccgaa
     3601 ctcatcgccg actacttcgg tcgggatggt ggaacggaac agcggctcgc cgtccggttg
     3661 gcgctcgttc atccgatagg gattgaagac aagttccgaa ccggcctcaa ccggatagag
     3721 cgacacgtcc agcgcgctga cgctgcggta catgattgcg acgtcggtcg ggaagctggt
     3781 actcagttgg gcctgctgga agggcaggtt gaacgaggcc agcggcgggg tgggggcgct
     3841 ggtgaaagtg aaggtgtaat cttcgcccag ggtgttgccg taggggtcgg cggccgtggc
     3901 gggcacggtg acggtgtatt cggtattggg gattaacgtg aactggacgt agaccgaata
     3961 actgccttcc cattcgttga tgaagtagtc ggccttctcc ggtgcgggcg agatgacgaa
     4021 ctgatcctcg atggtcgcat cgttcatcgg cgaggcgaat tcgacggaga agccgttgcc
     4081 ccagacgtcg gccatcccgc cgtcaatcgg cgaggtgctg atgacggccg ggaaggggat
     4141 ggtggtgaag cggctggtgt ggacgcgggc catggcggcc gcgccgttgg ccgaggtggc
     4201 cgaggaagcg acggccaggg tgtactccgt ctccagcgcc aacggctcgg ccgggcgcag
     4261 cacgacgacg cgatccccgt cctgccagtc gaactcgacc gccgtcgcgc cggccgtcgt
     4321 cgcgccggcc gcttcgctca ggttgatggc ggcttcggtg gcggcggtgt ccatgggcat
     4381 gttgaaggtg acggtgatgg gcgtggtggg ggtgacgcgc gccgcgtcgt tggccggctc
     4441 cacggtgacc acttcggggc tgacggtggt gaactgccag gtgggcgggg cgtcgatgag
     4501 gccgccggtg acgtcggtca gggccgggtt gacgctgacg atgtaggtgg tggctccggc
     4561 gaaggccggt tcgggcgtga agcgatagat gctggtcgat gtccattcgc ccgcgccctc
     4621 aatgggcggg tcgaaggcca gcggctgggg cagcccggcc tgctcgccgg agctgacgag
     4681 gggcacgacc ggcttgtcga agacgacggt caccacggcg tcggtctgca ccgagcgggc
     4741 gccgtcggcg gggatggtct gggtgacgcg cagcgggccg gtggcctgga aggcgaattc
     4801 gaccgcctct tgaagggttt ggccgttcat cgaggcggcg ctatcggcga tgcggacgtg
     4861 gtaggcctgg ccgggttcca gcgcgccggc gggggtgaag acggccgtgt cgggcgcggg
     4921 ccagctcatg gctccggcga cggccggctc gatggcgaag gcggcctcga ccgagggctg
     4981 atccatcggc tggtcgaagc gcacggtgat gggcgcgttg gcggccactt cctgaccggg
     5041 cagcgggtcg ccggtgacga cctggggtgg ccagtcgatg tcttcaaccg gaacgggccg
     5101 gacgacggtg gcggccgttg gttccggttc ggccggggtt gatggggcgt cggtcggttc
     5161 ggcgacttcg gcttccacgg cggcggcagt cggggcgacg gtcggcacgg tttccgtccg
     5221 tccgcgacga caagcggcca gagcgagcaa ggcggccatc agaataaaca gaatggtgaa
     5281 ttgtttgcgc aaattcatgc caatatcctc atacgcttga aaggacgatt agggcggagc
     5341 gctgccgggt ctgcacgcaa aaagccggga gcgcgggcct tgtaccttct ggacgatgga
     5401 tcagggggga tggttccaaa atacgagagg acagatcgtc cagtatcgac attgtaacat
     5461 aaaaacaaag tgacggatga cgaaaagcag ggtttttcag tagtccgttc caattgtgca
     5521 aagcggcctt atatggcatg acacatgcat gtaacataca tgtagccccg gtttcttaac
     5581 cgggtgatta cccttgccgc ggcgccgggt aagaatccgg cgtta
//
LOCUS       DAOMQG010000221         5580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6138_length_5580_cov_7.750407, whole genome shotgun sequence.
ACCESSION   DAOMQG010000221 DAOMQG010000000
VERSION     DAOMQG010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_5580_cov_7.750407"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="rny"
                     /locus_tag="PLC06_11520"
     CDS             <1..950
                     /gene="rny"
                     /locus_tag="PLC06_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRO91118.1"
                     /translation="VADMVVSVVPLPSDEMKGRIIGRNGRNIRAFEQAAGVDIIVDDT
                     PEAVTVSSFDPVRREVARRALAKLITDGRIHPSRIEKLISDAQDEVERVIKESGEQAA
                     YEANVHGLHPEILKMLGRLKYRTSYGQNQLQHSVEASHIASMLAAELGANIETAKMGA
                     LLHDLGKAMDHDQEGTHALLGAEFAKRFKVPAIVVNAIASHHHEVEQESIEAIIVEAA
                     DAISGARPGARRENLENYIKRVRTLEEIAASFNGVESAYALQAGREIRVLVKPNQIDD
                     LAAVRLSKEISKNIEDSMQYPGQIQVTVIRETRAVGFAK"
     gene            1083..1931
                     /locus_tag="PLC06_11525"
     CDS             1083..1931
                     /locus_tag="PLC06_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRO91119.1"
                     /translation="MSINSVAVIGSGTMGRGIAHAVALAGHPVILFDLTEGLLEIAIR
                     SIQGAIDEGVRRGKTRPEIAQRAKESLTLTTRLEDTAVVDLVIEAVPEQLELKRDIFG
                     RLDALAPIHTILASNTSSLSISAIAAATQRPERVIGLHFFNPAHLMRLVEIIRGDFTD
                     HATTSTCRAFVETLGKTPVLCKDSPAFIVNRIARPFYGEALRMLGEGVADHENIDRLI
                     SSLGFRMGPFELLDLIGIDVNFAVTQSVYNAYFQDPRYRPHPIQQRMVDSGRLGRKSG
                     RGFYSY"
     gene            2184..3215
                     /locus_tag="PLC06_11530"
     CDS             2184..3215
                     /locus_tag="PLC06_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRO91120.1"
                     /translation="MEQNTPKRQWQIWVGILVGLACLAAVFFFYARPGEIIENLRHIH
                     WLYLFLGALGVVAFLLLRAVRWRFMLDNSVPYGQVFHIQNIGYMLTQLLPLRLGDVAR
                     AILIGNVPPVTLTQGISTMVVERLLDMLFIVVLLPFTLSGVTALPEWMRSFALASGFA
                     AIAGIVILVMAANQRRRALDILDRLMARLLPFLNRERWLHRFDGLLVGLKSLTTLRDG
                     FTLTILSILIWIPIIVAYFFIMMAVGLEPTWVMAGFVVCAAAFSVAVPSTPGQAGPYH
                     FAVITALQLYGQPAVESASFAFLYHVANIVIMIIFGIIGILGTGVTFRNVLSSAQAFV
                     AGQRADQTP"
     gene            3285..4520
                     /locus_tag="PLC06_11535"
     CDS             3285..4520
                     /locus_tag="PLC06_11535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91121.1"
                     /translation="MKILEVLTYYRPWVSGLTIYVERLSRALVEQGHEVTVLTSQYDP
                     ALPRNETMHGVNVVRVPVAFRISKGVIMPGFGPMAWKLARQTDVMHLHLPQFDAPGLA
                     TRGRLLRKPVVLTYHCDLQLPVGFFNKVVDKVVQFQNQTAGKLADVVATYTRDYAMHS
                     PYLSQFVDRKLVIIPPPITLDPASDEVVRAFHEKHALGDRPVIGISARLAAEKGVEVL
                     LHALPRVLEKYPDLLVLHAGPYKNILGEEAYANRLAPLFEQFKAHYKLLGTLQGDELT
                     AFYRNLDILCICSLNSTESFGLVQIEAMRNGVPVAACNLPGVRQPVTMTGMGEVTAIG
                     DSDALAEALIRILDDREQYVRSPELISESFDPAQTAGEYVRIFEALQQGQRPTKSIEP
                     PAYDRLRAMRDEALRPTTK"
     gene            4517..5374
                     /locus_tag="PLC06_11540"
     CDS             4517..5374
                     /locus_tag="PLC06_11540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO91122.1"
                     /translation="MTTSDTTDDLLWQQLKTLPAFRALLRAVEARFYEQIPLPDPILD
                     IGCGDGNFAQLALPGRRITAGIDPWWRPLNKAVKAGNYELPIQALGDNLPFPDNYFAS
                     GFSNSVLEHIPDIQPVLNEIGRVLQPGAPFLITTISHYFTEFLGGGAFFDRLGLNGLA
                     DRYRDFFNRIARHAHADSPETWANRLAEAGFTIERWQYYFSREALHALEIGHVQGLPA
                     AAMHALTGHWIIAPWESSLGPTERWVRPFFEEEPQLDNGACLLLVARKGYNTAVKSLL
                     PPPNPLPIR"
BASE COUNT         1112 a         1765 c         1520 g         1183 t
ORIGIN      
        1 aggtggccga catggtcgtc tccgtcgtac cgttgccgag cgacgagatg aaggggcgca
       61 tcattggccg caacggccgc aatatccgcg ccttcgaaca ggccgcgggt gtcgacatca
      121 tcgttgacga cacccccgaa gccgtcactg tgagcagctt cgacccggtt cggcgtgagg
      181 tcgcccgccg cgctctggcc aagctcatca ccgacggccg catccacccc tcccgcatcg
      241 agaaactgat cagcgacgcg caggacgaag tcgaacgagt gatcaaggag tcgggcgaac
      301 aagccgctta tgaagccaac gtccatgggc tgcatcccga aatcctgaag atgttgggtc
      361 gtcttaaata ccgcacatcc tatggacaaa accagctgca gcactccgtc gaagcgtccc
      421 atatcgcctc catgcttgct gcagaattgg gagccaacat cgagacggcc aagatgggcg
      481 ctctgctcca cgacctgggc aaggccatgg accacgatca ggaaggcaca catgccttac
      541 tgggggctga gttcgccaaa cggttcaaag tgccggcgat cgtcgtcaat gccatcgcct
      601 cgcaccatca cgaagtcgaa caagagtcga tcgaggccat catcgtcgag gcggccgacg
      661 ccatttccgg cgctcgtccc ggcgctcgtc gcgaaaatct ggagaactac atcaagcgcg
      721 tgcgtacact tgaggaaatc gccgcctcgt tcaacggcgt cgaaagcgcc tacgcgcttc
      781 aggccggccg ggaaattcgc gtcctcgtga agcccaatca gatcgacgat ctggcggccg
      841 tgcgcctgtc gaaggagatc tccaagaata tcgaggactc catgcagtat ccgggccaga
      901 ttcaggtgac ggtcatccgc gagacgcggg cggtcggctt cgccaagtag cccacttcag
      961 gcaatatgag atagaggctc ccgcgcattg cagataacac gcgggagcct tttttgttct
     1021 tgcggtcgaa ctgttggttt tatggtttgg ctcgtgggaa tccatcccca aggagagtca
     1081 tgatgagcat taactccgtt gcagtgatcg gctcgggcac aatggggcgc gggatcgccc
     1141 atgctgtcgc gttggccggc cacccggtga tcctgttcga tttaacggag gggctactcg
     1201 aaatcgcgat ccgctccatt cagggtgcca tcgacgaggg cgtgaggcgc ggcaaaacca
     1261 ggccggagat cgctcaacgc gcgaaagagt cgctcacgct caccacaagg ctggaagata
     1321 cggccgtggt cgatctggtc atcgaagccg tgcccgaaca actggagttg aagcgagata
     1381 tctttggccg tctcgacgcc ctggcgccca tccacaccat cctggccagc aatacctcca
     1441 gcctgagcat cagcgccatc gccgccgcca cgcagcgccc cgaacgagtc atcggcctgc
     1501 acttcttcaa ccccgcccac ctgatgcgac tcgtcgagat catccgcggg gacttcaccg
     1561 atcatgcgac gacctcaacc tgccgtgcct ttgtcgaaac gctgggcaag actcccgtcc
     1621 tctgcaaaga ttccccagcc tttatcgtca atcgcatcgc ccgccccttc tatggtgagg
     1681 cgcttcgcat gttaggcgaa ggtgtggccg accacgagaa tatcgatcgg ctcatcagct
     1741 ccctgggttt tcggatgggg ccatttgagc tgctcgacct gattggcatc gacgtgaatt
     1801 tcgccgtcac ccaatccgtc tacaacgcct attttcagga cccgcgatac cgaccacatc
     1861 ccatccagca gcgcatggtc gacagcggtc gcctcgggcg aaaaagcggc cgcggcttct
     1921 atagctatta gcacagcatg tacgggcggg ataaccggcg tgagagcctt tgtccaggca
     1981 aaatgccttg ttcgccggtt atctcgcccc aactccgatc gccgacccat tccggcaaga
     2041 caacgcgcaa acctgtatgg tttcaacgtt tttctgccat gcgctctctc gtccaaataa
     2101 ctatcggtct gcaatacaat cgccctgtgc caacaagatc aaccctcctc ggccggggcc
     2161 gttgctggta taatgcccct tctatggagc aaaacacccc caagcggcag tggcaaatat
     2221 gggttggcat tctggtaggt cttgcctgtc tggccgccgt cttcttcttc tacgctcgcc
     2281 ccggcgagat catcgaaaac ctgcgccata tccattggct ttaccttttc cttggtgctc
     2341 tgggggtcgt agccttcttg ttgctgcgcg cggttcgctg gcgcttcatg ctcgacaata
     2401 gcgtgcccta cggtcaggta ttccacatcc agaatatcgg ctatatgttg acgcaactgc
     2461 tgcctcttcg actgggcgat gtggcgcggg ccatcctcat cggcaatgtc ccacccgtaa
     2521 cgctcaccca gggcatctca accatggtcg ttgaacgcct gcttgacatg ctgttcatcg
     2581 ttgtcctgtt gccattcacc ctgtcaggcg tcaccgccct gcccgaatgg atgcgctcct
     2641 tcgctctggc ctcagggttc gccgctattg ccggaatcgt catcttggtc atggccgcca
     2701 accagcgccg gcgcgccctt gatatcctcg atcgcttaat ggcccggctg ctcccctttc
     2761 tgaatcggga gcggtggctt catcgttttg acggcttgct cgtcggcctg aagagcctca
     2821 caaccctgcg cgatggcttc accctgacca tcctcagcat acttatctgg atccccatca
     2881 tcgtggccta cttcttcatc atgatggccg ttggtctgga gcctacctgg gtcatggcgg
     2941 gatttgtcgt gtgcgcggcc gcgttcagcg tggccgtccc ctcaacgccc ggacaggccg
     3001 gcccctatca cttcgcggtg atcaccgccc tgcaactata tgggcaaccg gcggtcgaat
     3061 cggccagttt cgcctttctc tatcacgtgg ccaatatcgt gatcatgatc attttcggga
     3121 tcattggcat tctcggcacg ggcgtcacgt tccgtaacgt cttgtcctcg gcccaagctt
     3181 tcgtcgccgg ccaacgcgcc gatcaaaccc cgtaactcct ggccgcggtt ttacatgcct
     3241 tcccgtgtat ataagccagg attaacgggc taacgcagaa agttatgaag atacttgaag
     3301 tattgaccta ttatcggccg tgggtaagcg gcctgacaat ttacgtggag cgcctgagtc
     3361 gcgccctcgt cgagcaaggc cacgaggtca ccgtgctgac ctcgcaatat gatccggcac
     3421 tccctcgcaa tgagacaatg catggcgtta acgtcgtgcg cgttccggtg gcctttcgca
     3481 tcagcaaggg ggtgatcatg cccggcttcg ggccgatggc ctggaaactc gctcgtcaga
     3541 cggatgtcat gcatttgcac ctgccacaat tcgacgcccc tgggctggcc acacgcggcc
     3601 ggttgctgcg caagccggtc gtcctcacct atcattgcga tctccaattg ccggtcggct
     3661 ttttcaataa agtggtcgac aaggtcgtgc aattccagaa tcagacggcg ggcaaactgg
     3721 ccgacgtggt cgccacctac acgcgcgatt acgccatgca ctcgccctat ttatcgcaat
     3781 ttgtcgatcg caagctggtc atcatccccc caccgatcac tctcgatccg gcttcggatg
     3841 aggtggtgag ggcatttcac gagaagcacg cattaggtga tcgacccgtc atcggcatct
     3901 cggcgcgact ggcggccgag aaaggggtcg aagtattgct ccatgctctg ccccgcgtcc
     3961 tggagaagta tcccgacctg ctggtccttc acgccggacc ctacaagaac atcctgggcg
     4021 aagaggcata tgccaaccgg ctcgccccgc tcttcgaaca gttcaaggct cactataagc
     4081 tgctgggcac gctccagggc gatgaactga ccgccttcta tcgcaacctc gatatccttt
     4141 gcatatgcag ccttaacagc acggagagtt ttggcctggt ccagatagag gctatgcgca
     4201 acggcgtgcc ggtggctgcc tgcaacctgc ccggcgtgcg ccaacccgtg actatgaccg
     4261 gcatgggtga agtcacggcc atcggcgact ccgacgcgct ggccgaggcg ctcatccgca
     4321 ttctcgacga ccgcgagcag tacgttcggt cgccggaact catcagcgag agcttcgatc
     4381 cggcgcaaac ggccggcgaa tacgtgcgaa tcttcgaggc gctacaacag ggccaaagac
     4441 ccacgaaatc catcgagccg ccggcctatg atcgcctgcg agccatgcgc gacgaagcgc
     4501 tcagaccgac aaccaaatga caacctccga cacgaccgac gaccttctct ggcagcagct
     4561 caagaccttg ccggcttttc gcgccctgct gcgcgccgtg gaagcgcgct tctacgaaca
     4621 aattcccctg cccgatccga tcctcgatat tggctgcggc gacggcaact tcgcgcaact
     4681 ggccctgccg gggcgacgaa tcaccgccgg gatcgatcca tggtggcggc cgctcaacaa
     4741 ggcggtcaaa gcaggaaact acgagctgcc tatccaggca ctgggcgata acctgccgtt
     4801 tcccgataac tacttcgcca gcgggttcag caactctgtg ctggagcaca ttcctgatat
     4861 ccagccggta ctgaacgaga tcggccgcgt gctccagcca ggcgcgccat tcctcattac
     4921 caccatcagc cactacttca cggagtttct gggcggggga gcattcttcg accggctggg
     4981 cctgaatgga ctggctgatc gctatcgcga tttcttcaac aggatcgccc gccatgctca
     5041 cgccgactcg cctgagacgt gggccaatcg tctggccgaa gccgggttta ccatcgagcg
     5101 ctggcagtat tacttctcca gagaggcatt gcacgctctg gagataggtc atgttcaagg
     5161 gttgccggcg gcggcaatgc acgcgctaac cggccactgg atcattgccc cttgggaaag
     5221 cagcctcggt ccgacagagc gctgggtacg acctttcttt gaagaggagc cccagttgga
     5281 caacggtgcc tgtcttttac tcgtagcgcg caaaggatac aatacggctg tgaaatcatt
     5341 gctcccgccg cctaacccgt tgccaattcg ctgattcgat tggatcattt ttgttctgaa
     5401 ttttgaaact tgaaactaat cagagcttga tgatgatcta cgaaccgcca cctgaccatc
     5461 aatctcccta ttggagcaaa accacgaagg taatcgtggt tgtattcttc atccttgcca
     5521 tcctttggct ggtcagtcgt tttcgcacga ttatcggcat gttggtgatg gcagtcgtgc
//
LOCUS       DAOMQG010000222         5573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6147_length_5573_cov_10.713846, whole genome shotgun sequence.
ACCESSION   DAOMQG010000222 DAOMQG010000000
VERSION     DAOMQG010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_5573_cov_10.713846"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..822
                     /locus_tag="PLC06_11545"
     CDS             10..822
                     /locus_tag="PLC06_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO91123.1"
                     /translation="MIDTTLFSLEGKTAVVTGALGLIGRKHCEALSAFGANVVVADLD
                     AGASAEFAAGLPTPSLGLALDVTDPESIAALRDAALARFGHLDVLVNNAAINDMFENP
                     AMAAEQSRFENYPLAMWQKSLEANVTGVFLCSQILGTPMARQGKGSIINIASTYGMVA
                     PDQSLYRTPDGRQPFYKSPAYPTTKGAVIAFTKFLAAYWGEAGVRVNTLSPGGVENNQ
                     QEYFIHNYSARTMLGRMASPADYQGALLFLAGDASAYMTGANLVVDGGWTAW"
     gene            854..1399
                     /locus_tag="PLC06_11550"
     CDS             854..1399
                     /locus_tag="PLC06_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRO91124.1"
                     /translation="MTNAPTTTIPPDELTRRAQAIRLLLTDSDGVLTDNGVYYSAAGE
                     ELKRFSIRDGMGVERLRKFTGVDVGIITGEFSGPVQRRAEKLAITELHLGIKDKPAVL
                     QDILSRLKLKPDQVAYIGDDTNDVEIMRQVGLAACPADATSFAQAVAHYICANQGGYG
                     AFREVAELIIAARAESAAPGE"
     gene            1407..2315
                     /locus_tag="PLC06_11555"
     CDS             1407..2315
                     /locus_tag="PLC06_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRO91125.1"
                     /translation="MTSSTKIKTGRRTIGDGEPVYIIAEIGINHNGSLDLARKLIDGA
                     IFAGADAVKFQKRTPELCVPRDQWNIERDTPWGRITYLEYRHRMEFTYEQYRTIDRYC
                     RARNLDWFVSCWDEAAVDFMEQFDTPMYKVASATLTDLPLLRKMKDTGRPLMLSTGMS
                     EMWQIQAAVDVAGHDDLMIAHATSAYPCPPEELNLRMIPALKATYPDIPIGYSGHEVG
                     LAPTWAAVTLGATFVERHITLDRAMWGSDQAASVEVMGFHNLVRNIRDIEMAMGDGIK
                     RIYPSELAAMKKLRRVDTTQPDHANS"
     gene            2302..3417
                     /locus_tag="PLC06_11560"
     CDS             2302..3417
                     /locus_tag="PLC06_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91126.1"
                     /translation="MQIRKLDTTQKSERARFVDFIFDLYKGHPLWVPPFRSEAMRILD
                     STKHPFYEHSCADFFVVEERDQVLGRIAMLENRSFNKFHNTKTAFFGYFEVVENIDVA
                     RLLLKKAIEWTKEHHLDTLIGPRGVIGIDGSVLVEGFDHRPAMTIPYNYPYYDAFIKD
                     SGFGKDTDYLSGYASGDHELPPRLYDIAAKVKAKRGFSIKTFASKDEIRQWIPQAMAV
                     HREAMSGLHSFFPPSEAETRAVINSLLMIIDPSLIKLVMKDDEIAGFLIAYHDVSAGL
                     QKAGGSLFPFGWFHILRDRQKTDWVNINGLGLLPQYRGLGANAVLYTELRDTIVAHGF
                     KHMDIVQVNETNFNSRSDMETMGIQWYKRHRHYKMVF"
     gene            complement(3426..3875)
                     /locus_tag="PLC06_11565"
     CDS             complement(3426..3875)
                     /locus_tag="PLC06_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRO91127.1"
                     /translation="MHTQQVADKPRRVCPACRYVYFTDPKVGVGVAVVEDGRLLLIKR
                     AMNPERGKWSLPAGFIDQGEDPKDAAAREALEETGLIVRIENVLDVFHNPPGAGGASV
                     FILYRAQIIGGELAAADDAADAGFFALDQLPELAFASTKAAVARLLA"
     gene            complement(3946..4848)
                     /locus_tag="PLC06_11570"
     CDS             complement(3946..4848)
                     /locus_tag="PLC06_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91128.1"
                     /translation="MFDFLRDRGSTAEEKRLEVLGAYLDDALTAAERVHLEGQLAADA
                     SMREELERLRVFKMQMRAMPRRRVPRSFALDPSLYARPKQQPLMQLYPVLRGATALTA
                     FFLIFTLALGMFRGQFAGGVAGSAPQAATAGVVEEEVAAFSVAESAPQVATVVTNEEA
                     RVMATSAPEMEAAPADLAAPAAVTEAESAELSAPAEGAAAVEAEPAQTELPETDLGAA
                     TAPEETADTTGETLLAPAPAGAGVESSAIATDSEQAAPAAAHRSRIDAYLLPIQILLA
                     ILFVLLLAFWLIARRRARQFQRDG"
     gene            complement(4859..5458)
                     /locus_tag="PLC06_11575"
     CDS             complement(4859..5458)
                     /locus_tag="PLC06_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO91129.1"
                     /translation="MNEETLIHQAQEGDIVAFNRLVVQHQELVFNVAYRIMGDPAVAE
                     DVAQETFITAYQSLKSFRGGSFKSWLIRVATNRCYDELRRRKRRPQTSLDEIMDENES
                     FAFLRSPNEGPEAHRQRVELALVIEHCLKGLPDDQRIVTVLGDVEGYDYQEIANITRV
                     SLGTVKSRLSRARAKLRDCLQQFGGELLPSAYRLDTRES"
BASE COUNT         1155 a         1788 c         1600 g         1030 t
ORIGIN      
        1 ttcatcaaca tgatcgacac aactctcttc tctcttgagg gcaagacggc cgtcgtcacc
       61 ggagcgttgg ggctaatcgg ccgcaagcat tgcgaagcgc tgtccgcgtt tggggccaat
      121 gtcgtcgtcg ccgacctcga cgccggcgct tcggccgagt tcgccgccgg gctgccgacc
      181 ccctcgcttg ggttggcgct ggacgtgacc gacccggaat caatcgccgc cctgcgcgat
      241 gcggccctcg cccgtttcgg ccatctggac gtgctggtca acaacgcggc catcaacgac
      301 atgttcgaga atccggccat ggccgccgag caatctcgct ttgagaatta ccctttggcg
      361 atgtggcaga agtcacttga ggccaacgtc accggcgttt tcctgtgctc gcaaatcctc
      421 ggcacgccga tggcccggca gggcaagggc agcatcatca atatcgcctc gacctacggc
      481 atggtggccc ccgatcaatc gctgtacagg acgcccgacg gccggcagcc gttttataaa
      541 tcacccgctt accccacgac caaaggcgcg gtcatcgcct tcaccaaatt cctggccgcc
      601 tactggggag aggccggcgt gcgggtcaat acgctatcac ccggcggcgt cgagaacaat
      661 cagcaggagt atttcatcca taactactcc gcacggacga tgctcggccg catggcctcg
      721 ccggccgact atcagggagc gctcctcttt ctggccggcg acgcctcggc ctacatgacg
      781 ggggccaatc tggtcgtcga cggcgggtgg acggcatggt aagcggacgg tcaccaataa
      841 tggacgccgg cgaatgacaa acgctccaac aacaacgatc ccgccggatg agctgacccg
      901 ccgcgcacaa gccatccgcc tgctgctcac cgacagcgac ggcgtgctga cggacaacgg
      961 ggtatactat tccgccgcgg gcgaggaatt gaaacgcttt tccatccgcg acggcatggg
     1021 cgtcgagcgc ctgcgcaaat tcaccggcgt ggatgtcggc atcatcaccg gcgaattttc
     1081 cggcccggtg cagcgtcgcg ccgagaagct cgccatcacc gagttgcatc tgggcatcaa
     1141 ggacaagccc gccgtgctgc aagatatttt gtcccggctg aaattgaaac cggatcaggt
     1201 ggcctatatc ggcgacgaca ccaacgacgt tgagatcatg aggcaggttg ggctggccgc
     1261 ctgtccggcc gatgcgacgt cattcgccca ggctgtggcc cattatatct gcgccaacca
     1321 aggtggatat ggcgccttcc gcgaagtagc cgaactcatc atcgccgcgc gcgccgaatc
     1381 ggccgcacca ggagaataaa ttatctatga caagttccac caaaattaaa acaggccgcc
     1441 gcaccatcgg tgacggcgag ccggtctaca tcatcgccga gatcggcatc aaccataacg
     1501 gctcgctgga cctggcccgc aagctgatcg acggggccat ctttgccggg gccgatgccg
     1561 tcaagttcca gaaacgcacg cccgagcttt gcgtcccgcg cgatcaatgg aacatcgagc
     1621 gcgacacacc ctggggccgg attacctatc tcgaataccg gcacaggatg gagttcacct
     1681 acgaacaata tcgcaccatc gaccgttact gccgggcgcg caatctggac tggttcgtct
     1741 cctgctggga tgaggcggcc gtcgatttca tggaacagtt cgacacaccc atgtacaagg
     1801 tcgcgtcggc gacgctgacg gatttgcctt tgctgcggaa gatgaaggat accggtcggc
     1861 cgctcatgct ctccaccggc atgtcggaga tgtggcaaat tcaggccgcg gtagacgtag
     1921 ccgggcatga tgatctcatg atcgcccacg caacatccgc ctacccgtgc ccgcccgaag
     1981 agctgaacct gcggatgata ccggcgctaa aggcgaccta tcctgatatc ccgatcggct
     2041 attccggcca cgaagtcggc ctggccccca catgggcggc cgtcacgctt ggcgcaactt
     2101 tcgtcgaacg ccacatcacg cttgatcgtg cgatgtgggg ttccgatcag gccgcctcgg
     2161 tcgaggtgat gggctttcac aatctcgtcc gcaacattcg ggacattgaa atggccatgg
     2221 gcgacgggat caagcgcatt tatcccagcg aactggcagc catgaagaaa ctgcgccgcg
     2281 tagacacaac ccaacccgac catgcaaatt cgtaaactgg acaccacgca gaaaagcgag
     2341 cgcgctcgct ttgtcgattt tatattcgac ctgtacaagg gtcacccgct gtgggtgccg
     2401 ccgtttcgct ccgaggcgat gaggattctt gacagtacaa agcacccatt ctatgagcat
     2461 tcctgcgccg acttctttgt cgtcgaggaa cgggatcaag tgctcggccg catcgccatg
     2521 ctcgagaacc gcagcttcaa caagttccac aacacgaaga ccgcattctt cggctacttc
     2581 gaggtggtcg aaaatatcga cgtggcacga ctgttgctga aaaaggcgat cgagtggacc
     2641 aaagaacacc acctcgacac gctcatcggg ccgcgcgggg tgatcgggat cgacggcagc
     2701 gtcctggtag aaggcttcga ccatcgcccg gccatgacca tcccctacaa ctatccctac
     2761 tacgacgcct tcatcaagga ttccggcttt gggaaggaca cagactacct ctccggctac
     2821 gcgagcggtg atcatgagct gcccccccgc ctctacgata tcgccgccaa agtaaaggcc
     2881 aagcgtggct tctccatcaa gacctttgcc tcgaaagatg agattcgaca atggattcca
     2941 caagcgatgg ccgttcaccg cgaggcgatg agcggcctgc acagtttctt ccccccgtcg
     3001 gaagccgaga cgcgagcggt tatcaactcc ctgttgatga tcatcgatcc ctcgctgatc
     3061 aaactggtga tgaaggatga cgagatcgcc ggtttcctga tcgcctatca cgacgtgtcg
     3121 gccggactgc agaaagcggg cggcagcctt ttcccgttcg gctggttcca catcctgcgc
     3181 gaccgccaaa agaccgactg ggtcaatatc aacggcctgg ggttgctacc tcaataccgc
     3241 ggtctggggg ccaacgcggt gctctacacc gaactgcgcg acaccatcgt cgcccacggg
     3301 ttcaagcaca tggatatcgt tcaggtcaac gagacgaact tcaacagtcg atccgacatg
     3361 gagacgatgg gaatccaatg gtacaagcgc catcgccact acaagatggt gttctgaggc
     3421 cggcgtcacg ccagtaaacg ggcgacggcc gctttcgtgc tggcgaaggc aagttcgggc
     3481 aactgatcga gagcaaagaa accggcatcg gccgcatcgt cggccgccgc cagttcgccc
     3541 ccgatgattt gagcgcgata cagaataaac accgacgccc cgcccgcccc cggtggattg
     3601 tggaagacat cgagcacgtt ctctattcgc acgatcagcc ccgtttcttc cagagcctcg
     3661 cgcgccgccg catccttcgg gtcttccccc tgatcgataa agcccgccgg caggctccat
     3721 ttgccgcgtt cgggattcat cgcccgcttg atgagcaaca accggccgtc ctcgacgacg
     3781 gcgacaccca cgccaacctt cggatcggtg aagtaaacgt agcggcaggc ggggcacacg
     3841 cggcggggct tatccgcgac ttgttgggtg tgcatgggcg acccacagcg cgggcaaaag
     3901 gcgaaccatt cgggggcact cgcagccggc atactcacgg gtgaatcaac catcacgctg
     3961 gaattgccgc gctcgacggc gggcgatcag ccagaaggcc agcagcaaga caaacaggat
     4021 ggccagcaaa atctggatcg gcaacagata ggcatcgatc ctgctgcgat gggccgctgc
     4081 cggtgcggcc tgttcagaat cagtcgctat agcagaggat tcgactccag caccggccgg
     4141 cgcaggcgca agcaacgttt ctccggtcgt gtcggccgtc tcttcaggcg ccgttgcagc
     4201 ccccagatcc gtctccggca attcagtctg ggccggctca gcctcgaccg cggccgctcc
     4261 ttccgccggg gcagacaatt cggccgattc ggcctcggta acggcggcgg gagcagccag
     4321 atcggccggg gcggcttcca tctcgggggc ggacgtcgcc atcactctcg cttcttcatt
     4381 tgtgacgacc gtggcgacct gtggggcgct ctcagccacg ctgaaagcgg caacttcttc
     4441 ttcaacaaca cccgccgtag ccgcctgtgg cgccgacccg gctacgccgc cggcgaactg
     4501 accccggaac atacccaaag ccagggtgaa gatcaggaag aacgccgtca gtgccgtggc
     4561 cccgcgcaag accggataga gctgcatcag aggctgctgc ttgggtcggg catagagtga
     4621 cggatcaagc gcgaagctgc gcggtacacg tcgtcgcggc atcgcccgca tttgcatctt
     4681 gaacacgcgc aaccgctcca gctcctcgcg catgctcgcg tcggcggcca attgcccttc
     4741 cagatggacg cgctcggcgg ccgtgagggc atcatccaga taggcgccca gcacctccag
     4801 acgtttttcc tcagccgtcg atccgcgatc tcgcaaaaaa tcaaacatga tagtccgcct
     4861 aactttctct tgtatccaga cgatatgcgg acggcaatag ttccccgccg aattgctgca
     4921 aacagtcgcg cagcttggcc cgcgcccgac tcaggcgtga tttcacagtt cccagcgata
     4981 cgcgggtaat attggcgatc tcctgatagt catagccctc gacgtcaccc aggacggtca
     5041 caatgcgttg gtcgtcgggc aggcccttca aacagtgctc gatcaccagc gccaactcaa
     5101 cgcgctgccg atgcgcttcc ggcccttcgt tggggctgcg cagaaaggca aaggactcat
     5161 tctcatccat gatctcgtcg agcgaggtct gcggccggcg cttgcggcgg cgtagctcgt
     5221 cgtagcagcg gttggtggca acgcggatca gccaggactt gaagcttccg ccgcgaaaac
     5281 tcttcaaact ctggtaagcg gtgatgaacg tctcctgagc cacgtcttca gccaccgccg
     5341 gatcgcccat gatgcggtag gccacgttga agaccagctc ctgatgttgc accaccagcc
     5401 ggttgaaggc gacgatatcc ccttcctgag cctggtgaat gagtgtctct tcgttcatcg
     5461 ttttcaggcc gggtgcgatt gacagaaacc gggccttggt ctatactccg cccttgataa
     5521 gccatgccga agtggcggaa caggcagacg cgcacgactc aaaatcgtgt tcc
//
LOCUS       DAOMQG010000223         5568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6154_length_5568_cov_6.984219, whole genome shotgun sequence.
ACCESSION   DAOMQG010000223 DAOMQG010000000
VERSION     DAOMQG010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6154_length_5568_cov_6.984219"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLC06_11580"
     CDS             complement(<1..462)
                     /locus_tag="PLC06_11580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO91130.1"
                     /translation="MTRPTLSVVILNWNGRAWLEACLVALAAQEPPPDRVVLVDNGSG
                     DDSVAFTRERFPWVTIRENGGNLGFAAGNNAALRDDTAEIVVLLNPDVVLAPGTLAAL
                     AEALAADPRIGIAGAKLWYPDGETIQHAGGFITHPQALPGHYGIGAVDAGDY"
     gene            complement(475..1962)
                     /locus_tag="PLC06_11585"
     CDS             complement(475..1962)
                     /locus_tag="PLC06_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HRO91131.1"
                     /translation="MRVTRILGLTAFVTALTVMAIWALRPAVAQTSPVAAAADAAAPL
                     CRYGVNVISNQTFIDHDTTPLRMGWYLNYRATPNTNSPAGAEFVPVIRLSQTGETEFS
                     YTPSGAALQNAIADNPGARWLIGNEPDRAGVNAGQDNVEPHIYAAAYHALYYLIKQAD
                     PTARVIAGTIVQPTPLRLRYLDMVLNSYHQTFGEAMPVDGWSIHNFILNEVSCLYSES
                     LPDEEKIPCWGAEIPPGIDEVIGEIVDINDNDDFDRFKERVVRFRQWMKDRGYRETPL
                     YLSEYGILMPPDYGDPPFGPARVNTYMTRTFDYLTTATSATLGYPADGNRLVQKWSWY
                     SATDQHFNGWLFDSGLSAMGQNYATHTAAIAVENDLAPWRLMTDPGTLYYTGAPLTIQ
                     LQAVVSNAGNPAAKAGPAVVRFYDGNPTQGGQQIGVDQTVSLAGCGSNTIAAVQWANV
                     GPGLHQVYVVVDATNAIAETNEANNIQSFPVLVGTQRAFTPRIAR"
     gene            complement(1959..3458)
                     /locus_tag="PLC06_11590"
     CDS             complement(1959..3458)
                     /locus_tag="PLC06_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRO91132.1"
                     /translation="MNINDRPFSMKAVAGLIATLLVIVMLTPIVGSTGTRLAAIGPEP
                     ELAGQASRVFMPVALVGRPTKWAPDSIFGMQMYNDSRPGSRFHPSLMDSGATWLRIAV
                     NWDSVEPTNTTPDSYLWQYADQPLGAAVPSHGAGIRVIGTIETAPLWARYDSYRPDGP
                     INPGNMSDFQEFVGAIVERYDGDGYQDAPGSPVVDYWEFYNEPDRRFNSPDGRWGDHP
                     VEYAMMLAAVYPTVKYHNPQAQVVFGGIAYDFFTEMGGPFVRSFLDDVLNAGAGEYFD
                     IFNFHTYPAFADNWLPPTNSNQGAGLYEKAQHLRQKLAGHGLNKPIIITEAGWRSNST
                     ATSVGSEEIQARYVVQLFTQSLAADLDTMIWWMLSDPGTAEGDYGLVTRDNPPYRKLS
                     FTAYQTIVNQLTGKPFVRALPSSETQHTLMEAYQFRDTSTGRAHYVAWLNPVSTNAKA
                     GLSIAAEEVRPTNLYGVAGPIITDAADGADDGRVRVTISGQPVYLEVLR"
     gene            complement(3497..4627)
                     /locus_tag="PLC06_11595"
     CDS             complement(3497..4627)
                     /locus_tag="PLC06_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91133.1"
                     /translation="MTETITDKTRPIPIIAAVAVLLITVVLLLGRPAAAAPDATDAFV
                     TAYPIPTVGSEPTHIIALGPNQVWYTMQNANAIGKLVVAGNGQATHTKYDIPTANSAP
                     YDLAWDGQYIWFTERLGNKIGRLNPANGQITEYPIPTASSQPMGIAVAPNGHVWFAEK
                     GGDKVARFRPATGTFDEYSYTTPGAKPEDVAVSNDDSIWFTAPGLKSVIRLLPNKPQN
                     QRFEPISVSNYGQSPLEPGRIAVLGAFPYDFPWITVPSKDYIGPYFGGTTGTWGWYAL
                     NNQNTGINSIFLQLAGNTYRTWFTEPNGNRAGLLITHKESSHVVTLFEIPLPGTNPRP
                     AGIVVDSGGTAWIAAPGTNAILAWRSPYFNQTFLPTLFRPRP"
     gene            complement(4751..>5568)
                     /locus_tag="PLC06_11600"
     CDS             complement(4751..>5568)
                     /locus_tag="PLC06_11600"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HRO91134.1"
                     /translation="AIHVHPDILIVDEILAVGDQSFQTKCIDRITEMKRAGVTILFIS
                     HDLKNVAHLCSDIVWMEHGEMRAVGPVDSVMDQYRNHLFNQTGRQMAVMNTSGGYRRW
                     GTKQIEITGVRLLDASGAPTTIFRTGEKMAVEISYIAHEPIEEPEFGLALHRHDGVHV
                     TGPNTHAAGLELGVVSGPGIVRYDIRALSLLPGRYNLTAAVHDSEAPIAYDYHQEAWS
                     FRVVDGGTPAAEGIVLLDADWQYVPAAPESTQPCGQPAIEPVTAGSHAPHSLA"
BASE COUNT          997 a         1558 c         1878 g         1135 t
ORIGIN      
        1 ataatcgccc gcatccaccg cgccgatgcc gtagtggccg ggcagcgcct gggggtgggt
       61 gatgaatccg ccggcgtgct ggatcgtctc gccgtcggga taccataatt tggctccggc
      121 gatgccgatg cgcgggtcgg cagccagcgc ttcggccaac gccgccagcg tgcctggggc
      181 gagcacgacg tcggggttga gcagcacgac gatctcggct gtgtcgtcgc gcagggcagc
      241 gttattcccg gcggcaaagc ccagattgcc gccgttctcg cggatggtga cccacgggaa
      301 ccgctcgcgg gtgaaggcca cggagtcgtc gccggagccg ttatcgacga gcaccacgcg
      361 gtcgggcggc ggctcctggg cggccagcgc gaccagacat gcttccagcc aggcgcggcc
      421 gttccagttt agaatgacga cggaaagcgt gggtcgggtc aaggtgttgc tcggttatct
      481 ggcgattcgg ggagtaaagg cgcgctgcgt ccccaccaga acggggaacg actggatgtt
      541 attggcttcg ttggtttcgg cgatggcgtt ggtcgcatcg accacgacat agacctgatg
      601 cagcccgggg ccgacgttgg cccattggac ggccgcgatt gtgttgctgc cgcaaccggc
      661 caggctcacc gtctggtcga cgccaatctg ttggccgccc tgggtcgggt tgccgtcata
      721 gaagcgcacc acggccggcc ccgccttggc cgccggattg ccggcgttgc tcacgacggc
      781 ctgcagctgg atcgtgagcg gcgctccggt gtagtagagg gtgccggggt cggtcatcag
      841 tcgccacggg gcgaggtcgt tctcgacggc tatggcggcc gtatgggtgg cgtagttctg
      901 gcccatcgcg ctcaatcctg agtcgaagag ccaaccgtta aagtgctggt cggtggcgct
      961 ataccaggac catttctgca ccaggcgatt gccgtcggcc gggtagccca gcgtcgcgct
     1021 ggtggcggtg gtcaggtaat cgaaggttct ggtcatgtag gtgttgacgc gcgccggtcc
     1081 gaatggggga tcgccatagt cgggcggcat gaggatgccg tactcgctca ggtagagcgg
     1141 cgtctcgcgg tagccgcggt ctttcatcca ctggcggaaa cggacaacac gctccttgaa
     1201 gcggtcgaag tcatcgttgt cattgatatc gacgatctcg ccgattacct cgtcgatccc
     1261 cggtggtatc tcggcccccc agcaaggtat tttttcttca tcaggaaggg attcagaata
     1321 gaggcaactg acctcgttga ggatgaaatt gtggatgctc cagccatcga cgggcatggc
     1381 ctcgccgaat gtctgatgat agctgttgag caccatatcc aggtaacgca gacgcagcgg
     1441 cgtgggctgg acgatggtgc cggcgatgac gcgggcggtg gggtccgcct gtttgatgag
     1501 atagtaaagc gcatgatagg cggcggcgta gatgtgaggt tccacgttat cctgcccggc
     1561 gttcaccccg gctcgatcgg gttcgttacc aatgagccag cgcgcgccgg ggttgtcggc
     1621 gatggcgttc tgcagtgccg cgccgctggg cgtataggag aactcggtct cgccggtttg
     1681 gctgaggcga ataaccggca cgaactccgc accggccggg ctgttggtgt taggggttgc
     1741 gcggtagttc agataccagc ccatcctcaa gggcgtcgtg tcgtggtcga tgaaagtctg
     1801 gttgctgatg acgttgacgc cataacgaca aagcggagcg gccgcgtcgg ccgctgcggc
     1861 gacgggcgac gtttgggcga cggccgggcg caaggcccaa attgccatca ccgtcaacgc
     1921 ggtcacaaac gcggtcagac ccaaaatgcg cgtcactctc atcgaagaac ctcaagatag
     1981 accggctgac cgctgatagt gacgcgcacg cggccgtcgt cggccccgtc ggccgcgtcg
     2041 gtgatgatcg gtccggccac gccgtagagg ttcgtcgggc gcacttcttc cgcggcgatg
     2101 ctcagcccgg ctttagcatt ggtgctcacc gggttgagcc aggcgacgta gtgagcgcgg
     2161 ccggtggagg tgtcgcggaa ttgataggct tccatgaggg tatgttgtgt ttccgaggac
     2221 ggcaacgcac ggacgaacgg ctttccggtc aactggttga cgatggtttg ataggcggtg
     2281 aaagacagct ttcgataggg aggattgtct cgcgtaacca atccataatc accttcggcc
     2341 gtaccgggat cgctaagcat ccaccagatc atggtgtcca ggtccgctgc cagactttgg
     2401 gtgaataatt gaactacgta acgtgcctga atttcctcgc taccgactga cgtggcggtg
     2461 ctattgctgc gccagccggc ctcggtgatg ataatcggct tgttaagccc atggccggcc
     2521 aacttttggc gcaagtgctg cgctttctca tagaggcccg ccccctgatt ggagttggtt
     2581 ggcggcaacc agttgtcggc gaaggcgggg taggtatgga agttgaagat atcgaaatat
     2641 tcgcctgcgc cggcattcag cacatcgtcg agaaaggagc gcacaaacgg cccgcccatc
     2701 tcggtgaaaa agtcgtaggc aataccgccg aacacaacct gggcttgggg attatggtat
     2761 ttcaccgtcg gatagacggc cgcgagcatc atcgcgtatt cgaccggatg atctccccaa
     2821 cggccgtcgg gcgagttgaa tcgtcgatcg ggttcattat agaactccca gtaatcgacg
     2881 acgggtgagc cgggcgcgtc ctgataacca tcgccatcgt agcgctccac aatcgcgccg
     2941 acgaactcct ggaagtcgct catattcccc gggttaattg gcccgtcagg cctgtagcta
     3001 tcgtaacggg cccagagcgg cgcggtttcg atggtgccga tcacccgtat gcccgcgcca
     3061 tgggacggca cggccgcgcc gagcggctgg tcggcgtatt gccacaggta gctgtcaggc
     3121 gtagtgttcg tcggctctac cgaatcccag ttgacggcga tgcgcagcca tgtggctcca
     3181 ctgtccatca ggctgggatg gaaccgcgaa ccagggcgcg aatcgttgta catctgcatg
     3241 ccgaagatgg agtccggcgc ccattttgtt ggccgaccga cgagagccac cggcatgaac
     3301 acccgcgagg cttgcccggc caattccggc tcggggccaa tggccgccag acgggtgccg
     3361 gttgagccaa ctatcggggt cagcatcaca ataaccagta aagtggcaat caggccggcc
     3421 acggctttca tggagaatgg tcggtcatta atattcataa ctatcaataa atcctgtggt
     3481 gaggtcgctt gttcggttat gggcggggac gaaacagagt cggaaggaat gtctggttaa
     3541 aatagggcga acgccaggcc aaaatcgcgt tcgtacccgg cgcggcgatc catgccgtgc
     3601 cgccggaatc gacaacaatg ccggccggcc gcgggttggt gccgggcaat ggtatctcaa
     3661 aaagtgtgac cacatggctc gattccttgt gggtaatcaa tagcccggcc cggttgccgt
     3721 tcggctcggt gaaccaggta cgataagtat tgccggccag ctgcaaaaag atgctgttga
     3781 tgccggtgtt ctggttattg agggcgtacc agccccaagt acccgtcgta ccgccgaaat
     3841 aggggccgat atagtccttt gacgggacag tgatccaggg gaagtcgtag gggaatgcgc
     3901 ccaacacggc gatgcggccc ggctccaggg gcgactgtcc ataattggag acggaaatcg
     3961 gctcgaagcg ctggttttgc ggcttattag gcagcagtcg gatgacggac ttcaggcccg
     4021 gcgcggtgaa ccagatgctg tcgtcgtttg atacggctac gtcttcgggt ttggctcccg
     4081 gcgttgtgta gctgtactca tcgaatgtgc cggtggccgg cctgaagcgg gccaccttgt
     4141 cgccgccctt ttcggcaaac cagacgtggc cgttgggggc gacggcgatg cccatcggtt
     4201 ggctgctggc ggtggggatg gggtactcgg tgatttggcc gttggccggg ttcaggcggc
     4261 cgatcttgtt gcccagccgt tcggtgaacc agatgtactg accgtcccag gccaggtcgt
     4321 agggagcgct gttggccgtg ggtatgtcat atttggtgtg ggtcgcctgc ccgttgccgg
     4381 ccaccacgag cttgccgatg gcgttggcgt tttgcatggt gtaccagacc tgatttggcc
     4441 ccagggcgat gatgtgggtc ggttcactgc cgacggtggg gatggggtag gcggtgacga
     4501 aggcgtcggt tgcgtcgggc gcggcggcgg ccggccgacc cagcagcagg acgaccgtga
     4561 tcagtagcac ggcaacggct gcaataatcg ggataggtct cgtcttgtca gtgatcgtct
     4621 ctgtcatgat atttgttcct tctcccccga ataatgaccc ccttcggttt cgatgggcaa
     4681 gaactgccgt gcccagtata gcttgagccg tttgttaatt cggcaatcgt tcgcgaggtc
     4741 taacgcgttg ctaagccagg gagtgcggcg catgagaacc ggcagtgacc ggctcgatgg
     4801 cgggctggcc acagggttgg gtgctttcag gcgcggcggg cacatattgc cagtcggcgt
     4861 cgagcaggac aatgccctcg gccgcgggtg tgccgccgtc gaccacccgg aagctccagg
     4921 cttcctggtg atagtcatag gcgatggggg cctcgctgtc gtggacggcg gccgtcaggt
     4981 tatagcgccc cggcagcaac gacagggcgc ggatgtcata gcgcacgatg cccggcccgc
     5041 tgaccacgcc cagctccagg ccggcggcat gggtgttggg gccggttaca tggacgccgt
     5101 cgtggcgatg gagcgccagc ccaaattccg gttcttcaat gggttcatgg gcgatgtagc
     5161 ttatctctac cgccatcttc tcgccggtgc ggaagatagt agtgggcgcg ccggacgcat
     5221 ccagcaggcg cacgccggtg atctcgatct gctttgtgcc ccagcgccgg tagccgccgg
     5281 acgtgttcat gacggccatt tggcgaccgg tctggttaaa caggtggttg cgatattgat
     5341 ccatgaccga atcgaccggc ccgacggcgc gcatctcgcc gtgttccatc cagacgatgt
     5401 cgctgcatag atgggcgaca ttcttcaggt cgtggctgat gaagaggatg gtgacaccgg
     5461 cgcgtttcat ctctgtgatg cggtcgatgc atttggtctg gaatgactga tcacccacgg
     5521 ccagaatctc gtcgacgatg aggatgtcgg ggtggacgtg gatggcga
//
LOCUS       DAOMQG010000224         5560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6164_length_5560_cov_10.320073, whole genome shotgun sequence.
ACCESSION   DAOMQG010000224 DAOMQG010000000
VERSION     DAOMQG010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6164_length_5560_cov_10.320073"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLC06_11605"
     CDS             complement(<1..1094)
                     /locus_tag="PLC06_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91135.1"
                     /translation="MEHKRSHRGSRLPVLLLSALFLVLSLVLGAFVRPAAAAGWWDNN
                     WRYRTLVTVDANNYERQDKPVEVNLNFTPLLAAQGGSGALDPDSIRVIEIDNADDVID
                     ADVPFQFDKAGDYHDTNKARGTLVFLLEGTTAANATRRFHVYFETTDAGIAAPSFSDQ
                     VSLTDGVSHKGYQSVRIVTADAEYFYHKPGGGFATLLDQDNNDWIGWSSATGGAGDFR
                     GIPNMVHPNDGGFFHPGRNIVSTTVLSDGPIRASFKSVSNGGAWEVRWDVFPTYARMT
                     VVKKGATNFWWLYEGTPGGVLEPSVDRLTRSNGDDIPASGTWSTDIPGDEWIFVTDPN
                     VGRSLYLIHHQEDTKIDGYVPDTGNKMTIFG"
     gene            complement(1295..5320)
                     /locus_tag="PLC06_11610"
     CDS             complement(1295..5320)
                     /locus_tag="PLC06_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HRO91136.1"
                     /translation="MKNQWPKLIVFALVLLLSGLLINVRSVSAAGSTGSGSVSLTTLG
                     SPSTQNFDTLANSGTGIDMNIAGWYIDEVGSGGAADGKYRAGTGSDNNGDTYSFGAAL
                     TTERALGSLASGTVLPRFGASFTNNTGATIASLDINYTGEMWRAGVALRTTTDQINFA
                     YSTNATNLTTGTWNAVNNLDFISPALNVATAGALDGNAAANQTAKSFTITGLNIANGT
                     TFWLRWSDPDMSGSDDGLAVDDFSITPNGGGGDVAPTITATNPIDGAINVAANTNVTI
                     TFDETVVISGTVKVEGSLSGIQNVTPTTGDNTTFTLDPADFTAGETVTVTVLATQVTD
                     QDGTPNNMAADYVFNFTINSGVPVCDQAFTPIYDIQGSGAAVIINSEITTEGVVVGDF
                     EGASPALRGFFIQDATGDADPATSDGIFVFDSTNSNNVDVGDVVRVTGTPGENQGQSQ
                     IGFLNGPIVDCGTGAVTPVDVSLPVASATYLEQYEGMLIRLPQTLYVTEHFQLGRFGQ
                     VVMSSGGRLQQPTNVALPGAPALAIQATNDLNRIIIDDGYNSQNPDPILFGRGGNPLS
                     AGNTLRGGDTATNIVGVMTYTWAGNAASGNAYRVRPINALGGSVPNFQAVNLRPASAP
                     VPTGTLRVGGMNVLNYFNTFDGLPDSVDNCTGGVGGAPTDCRGADTQAEFDRQWPKTV
                     AALVGSGADVIGLGEIENDGYDALSAIAELTDQLNDATAPGTYAYIDVDAETGQLNAL
                     GTDAIKVGFIYKPGSVTPVGTTAALNTVAFVNGGDASPRNRPALAQAFEEVGTGAQFI
                     VVANHLKSKGSACTAPDAGDGQGNCNTVRTNAVSQLITWLGTDPTGTGDPDILIVGDL
                     NSYAKEDPIAALEAAGYINLIEQFGGVNAYSYAFNGQWGYLDHALANATMETQVTGTA
                     EWHINADEPNALDYNIDFKSPGQQISLYNDDEFRMSDHDLALVDLELDLPPDTTDPDV
                     TINQASGQSDPTSSSPINFTAVFSEPVTGFTDAGVTLGGTAGATTAVVTEIAPNDGTT
                     YNVAVSGMTVDGTVIASIPAGAAEDGAGNGNTASTSTDNEVTYTVPVVPVSLFISTTA
                     AGSVGPLSYGSEDILKWDGSAWSMWFDGSATGLTPVGKWKHNINALWIEDEGAGSVIL
                     SFTQNARFVPGIGPKVDGMDLVRWDGSAYTLYFDGSDVGLTLKTQEKIDGLHILPDGY
                     QPIPVSCPGGNLLISTQGPGRVPAFGGGQIKFGGEDILGFCATNLGSATAGWWYMVLD
                     GSSVGLPTNAIDSISANADGSVISFTTSRPINVPGANGGHSMVFEYDTVSGTFNGPVF
                     SAPAVGIDKQVDALQVIGDLP"
BASE COUNT         1063 a         1637 c         1719 g         1141 t
ORIGIN      
        1 ccgaagatgg tcatcttgtt gccggtgtcg gggacgtaac cgtcgatctt cgtatcttcc
       61 tggtgatgga tcaggtagag cgagcggccg acgtttggat cggtgacgaa gatccattcg
      121 tccccgggaa tatcggtgct ccacgtgccc gaggcgggaa tgtcgtcccc gttggaacgc
      181 gtcaggcgat caacgcttgg ctccagcaca ccaccgggcg tgccttcata gagccaccag
      241 aaattggtcg ctcctttctt cacgacggtc atccgggcgt aagtgggaaa cacgtcccat
      301 cgcacttccc aggcgccacc gttgctgacc gacttgaacg acgccctgat tgggccatcg
      361 ctcagcacgg tcgttgagac gatgttgcgg ccggggtgga agaagccacc gtcgttgggg
      421 tgcaccatat tggggatgcc ccgaaagtca cccgcgccgc ccgtggccga gctccagccg
      481 atccagtcgt tattgtcctg atccagcagc gtggcaaagc caccaccggg tttgtgatag
      541 aagtactccg catcggccgt gacgatccgc acgctctggt atcccttatg actcacgccg
      601 tcggtcaggc tcacctgatc gctgaaagag ggcgcggcaa ttccggcgtc ggtcgtctcg
      661 aagtagacgt ggaaacggcg tgtcgcattg gcggccgtgg tgccttccag caggaagacc
      721 agcgtacccc gtgccttgtt cgtgtcatgg tagtcgcccg ccttgtcaaa ttgaaaaggg
      781 acgtcggcat caatcacatc atcggcgttg tcgatctcaa tgacccgaat cgaatcagga
      841 tcaagggccc ccgagccacc ctgagccgcc agcagaggtg tgaagttcag attcacttca
      901 accggcttgt cctggcgttc atagttattg gcgtcaacag tgacgagcgt gcgataacgc
      961 cagttgttat cccaccatcc ggcggccgcg gccgggcgaa cgaatgcacc taatacgaga
     1021 ctcaacacta aaaacaatgc ggacagcagc agaaccggta gtcggctccc tcgatgacta
     1081 cgtttatgtt ccattcgacg cattttcctg gtcctgtaaa cagcaacaca atactcgatc
     1141 tcgatcggca atccccggat cggcattgga cactgtattt ttctatcctt acaacaagtt
     1201 tgaagagcgc gtaaagattg tataaagaaa gcggcgcgcc ggtcgggcgc gccgctttct
     1261 aattacctgt cagggcttga atggtttcaa gtcgctaggg cagatcgccg attacttgca
     1321 aggcgtccac ctgcttgtca atccccaccg ccggagcgga gaagaccggg ccgttgaacg
     1381 tgccactaac tgtgtcatat tcgaagacca tcgaatgacc gccattggcg ccggggacgt
     1441 tgatcggccg cgacgtcgtg aaggatatca cgctgccgtc ggcgttggcc gagatgctgt
     1501 cgatagcgtt cgtgggcaag ccgacagacg aaccgtcgag caccatgtac caccagccgg
     1561 ctgtggcgct gcccagattg gtggcgcaga agcccaggat gtcctcaccg ccgaacttga
     1621 tctgcccacc accaaacgcc ggcaccctgc cggggccttg ggtgctgatg agcagattgc
     1681 ctccgggaca gctcaccggt atcggctggt agccgtcggg caggatatgc agtccgtcga
     1741 ttttctcctg ggtcttcaag gtcaggccaa catccgaacc gtcgaagtag agggtgtagg
     1801 cggaaccgtc ccaccgcacc agatccatcc cgtcaacttt ggggccgatg ccgggcacaa
     1861 agcgggcgtt ctgggtgaag gagagaatga ccgagccggc accttcatcc tcaatccaga
     1921 gagcgttgat gttgtgcttc cacttgccga cgggggttag cccggtcgca gagccgtcga
     1981 accacatcga ccaggcgctg ccgtcccact tcaggatatc ttcactgcca tagctgagcg
     2041 gaccgaccga accggcggcc gtcgtgctga tgaacagaga aaccgggaca acgggcacgg
     2101 tataggtcac ctcgttgtcg gtcgaggtcg aagctgtgtt gccgttgccg gccccatctt
     2161 cagcggctcc ggcgggaatc gaggcgatca ccgtgccgtc gacggtcatg ccgctgacgg
     2221 ccacgttgta ggtcgtgccg tcattcggcg cgatctcggt gacgacagcc gtcgtcgccc
     2281 cggccgtgcc acccagcgtc acgccggcgt cggtaaagcc ggtcaccggc tcactgaaga
     2341 cggccgtgaa gttgatcggg ctgctgctgg tcgggtcgct ctggccgctg gcctggttga
     2401 tggtcacatc ggggtcggtc gtgtctggag gcagatccag ttccaggtca accagcgcca
     2461 gatcgtgatc cgacatgcgg aactcatcgt cgttgtaaag agagatctgc tggccggggg
     2521 acttaaagtc gatgttatag tccagggcgt tcggctcgtc ggcgttgata tgccattcag
     2581 ccgtacccgt cacctgggtc tccatggtcg cgttggccag agcgtgatcc agatagcccc
     2641 actgcccgtt gaaggcatag gagtaggcat tcacgccacc gaactgctcg atcagattaa
     2701 tgtagccggc cgcctccaac gccgcgatcg ggtcttcctt ggcgtaggag ttcaggtcgc
     2761 caacgatcag gatgtcgggg tcgcccgtgc cggtcgggtc ggtgcccagc caggtgatca
     2821 actgactgac ggcattggtg cgcacggtgt tgcagttgcc ctggccgtcg ccggcatccg
     2881 gcgcggtgca ggcgctaccc ttgctcttca ggtgattggc gacgacgatg aactgcgcgc
     2941 cggttcccac ttcctcaaac gcctgagcca gcgcgggccg gttgcgcgga cttgcatcgc
     3001 caccgttgac gaaggccacc gtgttcagcg ccgccgtcgt accgacgggg gtgacgctgc
     3061 ccggcttgta gatgaagccg accttgatgg catcggtgcc caaggcgttg agctggccgg
     3121 tctcggcgtc cacatcgatg taggcatagg tgccgggagc ggtggcgtcg ttgagctggt
     3181 cagtcagttc ggcgatggcg ctcaacgcgt catagccgtc gttctcgatc tctcccagac
     3241 cgatgacatc cgcgccggag ccgacgaggg cagctacggt cttgggccac tggcggtcga
     3301 actccgcctg cgtgtccgcg ccgcggcagt cggtcggtgc gccgccgaca cccccagtgc
     3361 agttgtccac gctatccggc aggccgtcaa atgtgttgaa gtagttcagc acgttcatgc
     3421 cgccgacgcg caacgtgccg gtcgggaccg gggccgaagc gggtcggagg ttgaccgcct
     3481 ggaagttggg cacgctgccg cccagcgcat tgatcggccg cacgcgatag gcgttgccgc
     3541 tggcggcgtt gcccgcccag gtgtaggtca tgacgccgac gatgttggtc gccgtgtcgc
     3601 cgccgcgcag cgtgttgccg gcgctcagcg ggttgccgcc gcggccaaac aggattgggt
     3661 cggggttctg gctattatag ccatcatcga taataatccg gttcagatcg ttggtggcct
     3721 ggatcgcaag agccggcgcg ccgggcaggg cgacattggt cggctgctgc aagcgcccgc
     3781 cggatgacat cacgacctga ccgaaacgac ccaactggaa gtgctcggtg acataaagcg
     3841 tctgcggcag gcgtatgagc atgccctcgt attgctccag gtaggttgcc gaagccaccg
     3901 gcagggacac gtcaaccggc gtgaccgcac cggtaccaca atcgacgatc gggccgttca
     3961 ggaagccaat ctgcgactgg ccctggttct cgcccggtgt gccggtgacg cgcaccacgt
     4021 cgccgacgtc cacgttattg ctgttggtgg agtcgaagac gaagataccg tccgaggtgg
     4081 cggggtcagc gtcgccggtg gcatcctgga taaagaaacc gcgcagcgcc ggggatgcgc
     4141 cctcgaagtc gccgacgacg acgccctcag tggtgatctc ggagttgatg atgacggccg
     4201 cgccgctgcc ctgaatgtcg tagatgggcg tgaaggcctg atcacaaacg ggcacgccgg
     4261 aattgatcgt gaaattaaag acgtaatcgg ccgccatatt gttcggcgtg ccgtcctgat
     4321 cggtgacctg tgtcgccaac acagtgaccg tgaccgtctc gccggcggtg aagtcggccg
     4381 gatcgagcgt aaaggtcgtg ttgtcgcccg tggtcggggt cacattctgg ataccgctga
     4441 ggctgccctc gaccttgacc gtcccgctaa taacaacagt ctcatcgaaa gtgatggtga
     4501 cattggtatt ggcggccacg ttgatggcac cgtcgattgg gttcgtggca gtgatggtgg
     4561 gggcgacgtc gccgccgccg ccgttcggcg tgatggagaa gtcatccacg gccaagccat
     4621 catcggaacc agacatatcc ggatcactcc agcgaagcca gaaggttgtc ccgttggcga
     4681 tgttcaggcc agtaatcgtg aagcttttag cggtctggtt ggccgccgcg ttgccatcca
     4741 gtgcgcccgc ggttgccacg ttcaaggccg gactaatgaa gtcaagattg ttgacagcat
     4801 tccacgtgcc tgttgtcagg ttggtcgcgt tggtgctata ggcaaagttg atctggtcgg
     4861 ttgtcgtccg gagggccaca ccggcacgcc acatttcacc tgtataattg atgtccagtg
     4921 atgcgatggt tgcgccggtg ttgttggtga atgaagcacc aaatctggga agaaccgtgc
     4981 cacttgccaa agatcccaag gcgcgctcgg ttgttagggc agcaccaaag ctgtacgtgt
     5041 cgccgttgtt atcgctgcct gtccctgccc ggtatttgcc gtccgcggcg cctccgctac
     5101 cgacttcatc gatgtaccag ccggcaatgt tcatatcgat gcccgtgccg ctgttggcca
     5161 gggtgtcaaa gttctgggta gacgggctgc ccagagtagt caggctgact gagccggagc
     5221 cggtgctacc ggcggccgac actgaccgca cattaatgag caaacccgag agcaaaagaa
     5281 ctagcgcaaa cacaatcagt ttgggccatt gatttttcat ctcttatatc cttatgtcaa
     5341 ttgagcttat ttgctggaaa gcgagggtta ttcccggcac attcgccgcg aatacagagt
     5401 gggaaatgaa aacaggggat gttcacgcaa tatagtctcc tttacatgag aatgatatgc
     5461 gccgatagtt tccctcggcg cgtgaattgt tagcccctac cgcccttcta acccctgttc
     5521 ctccatagtt taccagattt tgtattaagc aaaatgatca
//
LOCUS       DAOMQG010000225         5557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6170_length_5557_cov_11.732824, whole genome shotgun sequence.
ACCESSION   DAOMQG010000225 DAOMQG010000000
VERSION     DAOMQG010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6170_length_5557_cov_11.732824"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLC06_11615"
     CDS             complement(<1..748)
                     /locus_tag="PLC06_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRO91137.1"
                     /translation="MSTTFMASPSLLFTSESVTEGHPDKMCDQISDAVLDALLERDPK
                     ARVACETAIKTGFVLLLGEITTNAFVDFDKLVRQVVIDIGFDDSSKGFDGNTCGVQAA
                     IAAQSPDIAQGVNHALEVRKNGDSDAEVDAVGAGDQGMMFGFACNETDVLMPMPIYLA
                     HRLTRRLSEVRRNGTLPWVRPDGKAQVTVEYAYGKPKRVRTVLISTQHAPEIDQETIK
                     RDIYEYVIKPVLPAELVGEGRNIVVNPAKPS"
     regulatory      complement(852..952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1054..1845)
                     /locus_tag="PLC06_11620"
     CDS             complement(1054..1845)
                     /locus_tag="PLC06_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HRO91138.1"
                     /translation="MSLLNGKKVLIFGVANKYSIAWAIAETFAEQGAEVGISYGIPQL
                     EKRVRPLAEEIGATFVEKCDVTSDEEIDDLFAKAQDHFGEIDILVHAVAYAPSEELEG
                     RFYDTSREGFRIAMDISAYSFIALANRAYKLMPNGGSIITLTYYGAEKVVPHYNVMGV
                     AKAALEASTRYLAADLGPHNIRVNAISAGPIKTLAAAGIAGFRKMLGYVGERAPLRRN
                     IDQNEVAKTALWLCSDLGSGVTGEVVYVDAGYHILGMPEPPEKWE"
     gene            1999..3033
                     /locus_tag="PLC06_11625"
     CDS             1999..3033
                     /locus_tag="PLC06_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRO91139.1"
                     /translation="MDKPTPQNVSRSSVIISMLAIATLAFLLFRNGEKWLRGVLIYLS
                     QAGWARATVSNWGVAWRVASRFVSGTDREAAIATTRGLNDKGLLVTLDYLGESCTTPE
                     EALQARAEIVALLDDIEKHKLQANVSLKPTQLGLKLDPQLAYENIRVVVQRAKSVGNF
                     IRIDMEDHPTTDATLNIFRQLRYTDGLDNIGVVIQSYLYRSEDDIEQLIGDGARVRLC
                     KGAYAEPPEVAYPQKADVDANYLNLSRMLLSDKAHTNGVYPAFATHDPAMIEDIKRYV
                     GSNNIPLNSFEFQMLFGIRRDLQIALAQEGYRVRVYVPYGTAWYPYFVRRLAERPANI
                     WFFISNFVKA"
     gene            3044..3586
                     /gene="rfbC"
                     /locus_tag="PLC06_11630"
     CDS             3044..3586
                     /gene="rfbC"
                     /locus_tag="PLC06_11630"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRO91140.1"
                     /translation="MPLLTESSLIPGVINVQLRPFADERGRFIELFRKEWFPQRDWSN
                     VQSNRSESKVGVLRGLHYHFNQVDYWYVVSGRIRVGLADLRRSSIFYRRTTTIDLDAA
                     DNRGLFIPIGVAHGFLALTDVTLIYTVDNYYDGRDEFGLAWNDPELAVPWNFDGIPTL
                     SNRDLENPLFSAIPMQSLPD"
     gene            complement(3598..3993)
                     /locus_tag="PLC06_11635"
     CDS             complement(3598..3993)
                     /locus_tag="PLC06_11635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRO91141.1"
                     /translation="MTTEPRARFDWRRRLKSFIYAFEGWRHVLLTQHNAWIHAAISIV
                     VFALAIWLQLSRVEWSILILTIMVVWMAEFFNTAIEAVVDMTAPEYRPLAKVAKDVAA
                     AAVLVGALGAVIIGLLIMGPALWARLFTP"
     gene            complement(3983..>4258)
                     /gene="ybeY"
                     /locus_tag="PLC06_11640"
     CDS             complement(3983..>4258)
                     /gene="ybeY"
                     /locus_tag="PLC06_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRO91142.1"
                     /translation="PAGEPMPGNEGLLDYLGDIAIAVPVAERQASEKGHPLVAEIQLL
                     AVHGVLHLVGYDHLEADEKEEMWDAQRAILTRLGLENIQPTEDGHDD"
     assembly_gap    4259..4358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4359..5527)
                     /locus_tag="PLC06_11645"
     CDS             complement(<4359..5527)
                     /locus_tag="PLC06_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRO91143.1"
                     /translation="MGLISLGITILISLYWVRFQRQQFANGRYLGVLGGFILIFTLVG
                     KIMMASDMLAYWFPLAALSLLLAVIYDFRFSILITGLMAGLIGIISPNSLEMTVYLAS
                     GGILSVLTLKDTQRMAAFFRAGLLAAGGYFFVVVVFWLLAGSDKNPSDLAMPAISSLG
                     NGLISSALTLAGFYVLGGLFGIVTILQLQDLSRLDHPLLRELLRLAPGTYHHSIMVAN
                     LAEQAAERIGANSTLVRVSAFYHDVGKTVRPPFFVENQEGVNPHASLDPLTSARIVIK
                     HVADGIALARQYRLPFQVQDIIAEHHGTRPVKSFYRKAQEAAGEGVEIDIQAFRYPGP
                     RPRSREAAIVMLADAIDATSTALRPNTEKAIEKLVNTIIEEDILEGQLNDSKLSMG"
BASE COUNT         1288 a         1519 c         1411 g         1239 t
ORIGIN      
        1 ccgacggctt ggccgggtta acaacgatgt tccgcccttc accaaccagt tccgcaggca
       61 ggacgggttt gatcacgtac tcgtaaatat cgcgcttgat cgtttcctga tcgatttcgg
      121 gcgcatgttg ggtgctaatc agcacagtcc gcacgcgctt cggcttgcca tatgcatatt
      181 cgactgtcac ctgcgccttg ccatcaggac gtacccaggg caaagtgcca ttgcgacgaa
      241 cttcagacaa ccgacgagtg agccggtgcg ccagataaat cggcataggc atcagaacat
      301 cggtctcgtt gcacgcaaag ccgaacatca taccttgatc gcccgcgcca acagcgtcga
      361 cttcggcgtc gctgtcgcca ttcttgcgaa cttcgagcgc gtgattcaca ccctgggcaa
      421 tatcggggct ttgcgcggca atggcggcct ggacgccgca ggtattgccg tcgaatccct
      481 tgctgctgtc atcgaaaccg atgtcgatga cgacctgccg gacgagcttg tcaaaatcta
      541 caaacgcgtt ggttgtgatc tcgcccagca acaggacaaa gccggtcttg atggccgtct
      601 cacaggcaac acgcgccttg ggatcccgct caagcaaggc gtccagaaca gcatcgctga
      661 tctgatcgca cattttatcc ggatgccctt cagtgactga ttcagacgtg aagagcaggc
      721 tcggagaggc cataaaagta gtagacattg gttcttggtt acctcccaaa attgttagtt
      781 cggttggccg cgatataaca aaaaacgcct ctgagcaagt caagaggcgt agcgttgtgt
      841 ttcacttgta cctcttatct ttcagctttc gctgccggag ttagcacctt tccaaaatgg
      901 ttggttgctg cagtgtcaat gggccgggtc cctcgactgc tctagataag cgcgattgct
      961 ctatcgtaac gtacccaaca gattaatata accgcaagta gcgatttgtc aatatcatat
     1021 gccgcgtggc ttacgaacaa gattaagggg aactcactcc cacttctcgg gtggctccgg
     1081 catccctaat atatgatatc ctgcatcaac atagacgacc tctccggtga caccgctgcc
     1141 caaatcgctg catagccaca gggctgtctt ggctacctca ttctgatcaa tattgcgacg
     1201 gagtggtgct cgctcaccca cgtatcccaa catcttgcgg aacccggcga ttcccgcggc
     1261 agccagcgtc ttgatcgggc cggcagagat ggcattgact cggatgttat gtggccctag
     1321 atcggccgcc aaatagcgcg tacttgcttc cagcgcagcc ttggcaaccc ccatcacgtt
     1381 gtaatgggga acaactttct ctgcgccata gtaagtaagc gttattatgc tgccgccatt
     1441 gggcatgagc ttataggctc ggttggccag ggcgatgaaa ctatatgcgc taatgtccat
     1501 cgctatacga aatccttcgc gcgacgtgtc gtagaaacgc ccttccagtt cttctgaagg
     1561 cgcatacgca acggcgtgaa ccaggatgtc aatttcgcca aagtgatcct gggctttggc
     1621 gaaaagatca tcgatctcct cgtcggatgt gacgtcgcat ttttccacaa aagtagcacc
     1681 gatttcctcg gccaagggtc gaactcgttt ctcaagttgg ggaatcccgt aactaattcc
     1741 cacctcggct ccttgctctg caaacgtctc cgcgatggcc caggcaatag aatacttgtt
     1801 ggcgacgccg aagatcaaaa ccttctttcc gttcagtaaa ctcatgagaa atgtatctcc
     1861 cgatcgaact aggaattgca ctgattaatg ataacccgta cccactaaat aaggtacgtt
     1921 catggtggcg gcttgctcaa atggcgacat cgacatcaat agcgtgacga attattatgg
     1981 cgggagtaaa atttgctcat ggacaagccc accccacaaa atgtctctcg ttcgtcagtc
     2041 attatttcga tgctggccat tgcgaccctg gccttcctct tgttcaggaa tggcgagaaa
     2101 tggttgcgcg gcgttctcat ctatctgtca caggccggct gggcgcgggc taccgtcagt
     2161 aactggggcg ttgcctggcg cgttgcctca cgattcgtgt cgggcacgga tagagaggcg
     2221 gccatcgcga cgactcgcgg cttgaatgac aaggggctcc tggttacact cgattatctg
     2281 ggcgagagct gcacgacccc ggaggaggcg ttacaagcca gagccgaaat agtagcgctg
     2341 cttgatgaca ttgaaaagca caaattgcag gccaatgtct cgttgaagcc cacccagctc
     2401 ggcttgaagc tcgatcccca gttggcgtac gagaacattc gggttgtggt gcaaagggcc
     2461 aaatccgtgg gcaatttcat acggattgac atggaggatc accccacaac cgacgccacc
     2521 ctcaacatat tccgtcaatt gcgctacacc gatgggctgg acaacatagg cgtggtgatt
     2581 cagtcctatc tctatcgcag cgaggatgat atcgagcaat tgattggtga cggcgcgaga
     2641 gtcaggctat gtaagggagc ctatgccgag ccgcccgagg tcgcctatcc tcagaaagcc
     2701 gatgtggatg cgaattatct caatctgtca cggatgctgc tcagcgataa ggctcataca
     2761 aatggtgttt atccggcgtt tgccacacac gatcccgcga tgatcgaaga tatcaagcgc
     2821 tatgtcgggt cgaacaacat ccccctcaac tcattcgaat tccagatgtt attcggtatc
     2881 cggcgtgacc tccagatcgc cctggcccag gaaggatacc gcgttcgggt ctacgtccca
     2941 tacggcacag cctggtatcc ctattttgtg cgccgattgg ccgagcgccc ggcgaacatc
     3001 tggttcttca tcagcaattt tgtaaaagcg taagcattat cctatgccgt tattgactga
     3061 atcatccctc atcccggggg tcatcaatgt ccaattgcgt ccattcgccg acgagcgcgg
     3121 ccgctttata gagttatttc gcaaggagtg gttcccgcag agggactggt caaacgtgca
     3181 gtcgaaccgc tccgaatcca aagtcggggt gctgcggggt ctccattacc atttcaatca
     3241 ggtcgattac tggtatgtcg tcagtggccg tatccgggtt gggctggccg atttacgacg
     3301 atcttcaatt ttctaccgtc ggacgacaac gatagatttg gatgcggccg ataacagggg
     3361 gctattcatc cctattggcg tggcgcatgg atttttggca ctaactgatg tgacgctgat
     3421 ctacaccgtc gacaattact atgacggccg cgatgaattc ggcctcgcct ggaatgatcc
     3481 tgagttagcc gtgccctgga atttcgacgg tatccccacc ctctccaatc gcgatctcga
     3541 aaatccgcta ttcagcgcga ttccaatgca gtcactgccg gactgattca taccccgcta
     3601 cggtgtgaat aatcgcgccc acaaggccgg acccatgatt aagaggccaa ttatcaccgc
     3661 tccaagagcg cccaccagca cggccgcggc agccacatct ttagccacct tggccagggg
     3721 acggtactcc ggcgcggtca tatcgaccac cgcttcgatg gcggtgttga agaactccgc
     3781 catccagaca accattatcg tcagaattaa gatggaccat tcaacacgtg aaagttgcag
     3841 ccatatcgct agcgcaaata cgacaatgct tatggctgca tgaatccagg cgttatgctg
     3901 ggtgagcaac acatgacgcc atccctcaaa ggcatagatg aagctcttca ggcgccggcg
     3961 ccaatcaaaa cgcgctcgag gttcagtcgt catgcccgtc ttcagttggc tggatatttt
     4021 caaggccaag gcgcgtcagg atggcgcgtt gtgcatccca catttcctct ttttcatcag
     4081 cctcaagatg atcgtagcct accaaatgca atacgccgtg gacagccaaa agctggattt
     4141 cggctaccag gggatgcccc ttctcagacg cctgtctctc ggcgacgggc acggcaatgg
     4201 cgatatcgcc caaatagtcc agcaaccctt cgttgccggg cattggctca cctgccggnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc catgcttagt ttggaatcgt
     4381 taagctggcc ttccaggata tcctcttcga tgatggtgtt cacaagcttt tcaatcgctt
     4441 tctcggtgtt gggcctcagg gcggttgatg tggcatcaat cgcatcggcc agcatgacaa
     4501 tggctgcttc tcgcgagcga ggtcgcggcc ctggatatcg aaacgcctgg atatcaatct
     4561 caacgccttc gccggcggct tcctgcgcct tccgatagaa gctcttcaca gggcgtgtcc
     4621 catgatgctc ggcgatgata tcctgcacct gaaacggcaa gcggtattgc cgcgccagcg
     4681 caatcccatc ggccacgtgc ttgatcacga ttcgcgcgct ggtcaacggg tcaagcgaag
     4741 cgtgtggatt tactccttcc tgattttcga caaagaaagg tggccgtacc gtcttgccga
     4801 catcatgata gaaagcgcta actcggacaa gtgtactgtt cgcaccgatg cgttcggccg
     4861 cctgctcagc cagattggcc accatgatgc tgtgatggta tgtgccggga gccaagcgca
     4921 gcaattctcg caagagggga tggtcaaggc gtgacaagtc ctgaagttga agaattgtaa
     4981 caataccgaa tagtccaccc agaacataaa acccggctaa cgttaacgct gaggagatca
     5041 agccattccc aagggaagaa atggccggca tcgcaagatc cgagggattt ttatccgacc
     5101 ctgcaaggag ccaaaaaacg acgacaacga agaaataacc accggcagcc aaaagcccag
     5161 ctcggaagaa agcagccatc ctctgggtat cttttagagt caatacacta aggatgccac
     5221 cggatgccag atagacggtc atttccagcg aatttgggct gatgatgccg atcaatcccg
     5281 ccatcagtcc ggtaatcaga attgaaaagc ggaagtcata gatgacggcc agcagaagag
     5341 acaaggccgc cagggggaac caataagcca acatatcgct ggccatcatg attttaccta
     5401 ccagggtgaa gataaggatg aatccaccca acacacctag gtagcgccca tttgcgaatt
     5461 gttgtcgctg gaatcgaacc caatagagcg aaatcaggat cgttatcccc agcgagatca
     5521 accccatgct taaggcatgg agccagttcg tggactg
//
LOCUS       DAOMQG010000226         5529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6209_length_5529_cov_6.574351, whole genome shotgun sequence.
ACCESSION   DAOMQG010000226 DAOMQG010000000
VERSION     DAOMQG010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_5529_cov_6.574351"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLC06_11650"
     CDS             complement(<1..398)
                     /locus_tag="PLC06_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91144.1"
                     /translation="MNARKETAMAVKIRPSRRTRGMKGKTILTFGGGLLVAFIAVFLA
                     LAPVPKARAATTTINVCAQDATNGWNLLSYQMGYLRAKLDNPVNFGPVGTYGDFDFNY
                     IDIGTNVTEATLLSNACNIWFSGYEADSQYT"
     gene            782..1201
                     /locus_tag="PLC06_11655"
     CDS             782..1201
                     /locus_tag="PLC06_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRO91145.1"
                     /translation="MTYEEQQTIEKILRTARTIATVGYSADPAKKAHEVPEYLYAQGY
                     RVIPVNPKVGEALGEKGYASLADVPDAIDVVQIFRPGAEVGPIVDAAIAAGAGAVWMQ
                     IGVVNEEAAARARAAGLDVVMNQCMKVQHSRWAAPMI"
     gene            complement(1239..2486)
                     /locus_tag="PLC06_11660"
     CDS             complement(1239..2486)
                     /locus_tag="PLC06_11660"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRO91146.1"
                     /translation="MTTQQLAVQSTAHLTDQAQREQLQTELLVTSSTVIALLAMTWGL
                     IYMYFGAVNAGAIPIAYAAFSFASIGYFALTGRYHLFRFGQLLITLIFPALLMLVLGG
                     FVNSSGVILWSLTSPVGALLFSTRRRAAVWFMAFVAVVVVAGLIDGGAFGPALPEQAL
                     LSPRLIVTFFILNAIGPSVVVFLLLAHFTRQRDQAHDLLVMEQAKSDALLLNILPQSI
                     ARRLKDGQHNIAECIDDASILFADVAGFTPMSAELSAEYVVGLLNDLTSGFDDIIERH
                     KLEKIRTIGDGYMAASGVPTPRADHAHALADAALDMIAFTHELSARYGFPIRLRIGIN
                     SGAVMAGVIGRKKFSYDIWGDPVNVASRMESHGVAGAIQIAERTHMLIRDAFITEPRG
                     TIEIKGKGKMETWFLVGRKQDIA"
     gene            complement(2700..3872)
                     /locus_tag="PLC06_11665"
     CDS             complement(2700..3872)
                     /locus_tag="PLC06_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91147.1"
                     /translation="MSRGEFCISGNVPANGTYSFLITVSAPTVPQGEVRHATLRMTSN
                     RGGSLTFPITIVRGQAVVEMSKTCTPSVLRVRESTSCSITLTNTGFDDASFNLIDMLP
                     KQLRLTAGTVVGGTQIGRKNGVQASGVLQGAEPPDVNVTVDPLASPAGYYALGGAAVA
                     GFGDETIGNFNVPAFLYGGEVYTRIGVTSNGYAIVGGGTAADVTYINTNLPNATAPNN
                     VLAPFWTDLNVATAPVGGGIRAGILSGGGSSWIVVEWRNVPNYSNAAQKNTFQVWIGV
                     SGDVTPYEDISFTYGSAITGGDGGYLTVGAENSFGSRGGTVYYNGNGAPPSPSSGGYE
                     VLVSTTPPTPGESHTISFTAVGKRAGAWTNCAEMTSDVFAGTALACDSGRVNSRGN"
     gene            4026..>5529
                     /locus_tag="PLC06_11670"
     CDS             4026..>5529
                     /locus_tag="PLC06_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91148.1"
                     /translation="MVSKIGRIVVVIGLILVGTWLTIRSASPAAESSARVAAIRQAAP
                     ASRIGSSSAGQVSDMVVAEPRTVDLRSIPAGVYDPDNQYDRWQRGEIDLLENEGILSA
                     AEMAKLRADSERLPASGNITLADGADGIGSNAPSPAGASFDSIDYTDCCGGGGNVPPD
                     PELAVGPNHVIAVVNVALEIYNKNGNSLVGPTTFASFFNSDPNCTGVFDPNALYDEEA
                     DRYMVGIDADGFYYCIGVSQTGDPTGNWNIYSFPTASGADFFDYPHAGVGRDAIYMGA
                     NIFDTGFKESRVWAFDKWAMYSGASAAGVSKPLPATEDTPQPLNLHGWQQNTWPTSGP
                     HYFFTETDYNGATYSVWSWTNPFGGTSPSKVGTVNLTTFTGVSAGMPVAVPQNGGQTI
                     QGNDYRPQDFEYRNGYAWSISTVACNPGGGTVNCIRWAQINPATATIVDAGVYSSSGE
                     YRFFGDLAVNHCGDVAVGYTKSSTSMWPSVFYTGRKAGDAAGTLQAEAQLK"
BASE COUNT         1078 a         1660 c         1748 g         1043 t
ORIGIN      
        1 gtgtactggg agtcggcttc atagcccgag aaccagatgt tgcacgcgtt gctgagaagc
       61 gtcgcctcgg tgacgttggt acctatgtcg atgtaattga aatcaaaatc gccgtaagtg
      121 ccgacggggc cgaaattgac gggattgtcc agcttggcgc gcagataacc catctggtag
      181 ctcagaagat tccagccatt agtggcgtcc tgggcgcaga cattgatggt cgttgtcgcg
      241 gcccgggcct tgggcaccgg cgccagggcc agaaacacgg cgataaacgc caccagcagc
      301 ccgccgccaa acgtcaaaat cgttttccct ttcattcctc tcgtcctcct ggatggtctg
      361 atttttaccg ccatcgctgt ttctttccgc gcattcattt tcgatcgctc ccattcgtat
      421 gaccttgtat gccgggcaag gtcgttccaa taagccggcc gcggcaggcc gtttccggat
      481 ttaacgtagc acattatgcc tgtatcaaca ttacgatgac tattacgaca atatcttcat
      541 aggcaaatat actccatatt gggacatcga aaggacgggg cggggtgtat ttaatgtggc
      601 tgcggccgcg tcattcgatg tgcccgatcc atgtcgatgg gcataataca acagtttaca
      661 ggcctctgtc tgcaatctac ccgacacaac tcgaccaatt ttgactaatt tcggagcaaa
      721 attgcccaat ttgccgactc gacccacgac aaaccgcccg cgcctttgta taatcccgcc
      781 catgacctac gaagagcaac aaaccatcga gaaaattttg cgcaccgcca ggaccatcgc
      841 caccgtgggc tattcggccg acccggcgaa gaaggcccac gaggtgccgg agtatctgta
      901 tgcgcagggc taccgggtga ttccggtcaa tccgaaggtg ggggaggcgc tgggggagaa
      961 gggctacgcg agtctggccg atgtgccgga cgccatcgac gtggtgcaga tcttccggcc
     1021 gggagccgag gtggggccga tcgtcgatgc ggccatcgcc gccggggccg gggcggtgtg
     1081 gatgcagatc ggcgtggtca atgaggaagc ggccgcccgc gcccgcgccg ccgggctgga
     1141 tgtggtgatg aaccagtgca tgaaggtgca gcattcgcgc tgggccgcac cgatgattta
     1201 gcttttggaa accgggtttc ttccgggaaa cccggtttct atgctatatc ctgtttcctc
     1261 cccaccaaaa accacgtctc catcttcccc ttgcccttga tctcgatcgt gccgcgcggc
     1321 tcggtgatga acgcgtcgcg gatcagcatg tgggtgcgtt cggcgatctg gatggcgccg
     1381 gccaccccgt gggactccat gcggctggcg acgttgaccg ggtcgcccca gatgtcgtag
     1441 ctgaacttct tgcggccgat gactccggcc atcaccgccc ccgaattgat gccgatgcgc
     1501 agccgaatgg ggaagccata tcgggccgac agctcgtggg tgaaggcgat catgtccagc
     1561 gccgcgtcgg ccagcgcgtg ggcgtggtcg gcccgcggcg tgggtacgcc cgaggcggcc
     1621 atgtagccgt cgccgatggt gcggatcttc tccagcttgt ggcgttcgat gatgtcgtcg
     1681 aagccgctgg tgaggtcgtt gagcagcccg acgacgtact ccgcgctcag ctcggccgac
     1741 atgggcgtga acccggccac gtcggcgaag aggatgctgg cgtcgtcgat gcactcggcg
     1801 atattatgct gcccgtcctt caggcggcgg gcgatggact ggggcaggat gttgagcagc
     1861 agcgcgtcac tcttggcctg ctccatcacc agcaggtcgt gggcctggtc gcgctggcgg
     1921 gtgaaatggg ccagcagcag gaacaccacc accgacgggc cgatggcgtt gaggatgaaa
     1981 aaggtgacga tgagccgggg agacaacagg gcctgttcgg gcaaggccgg gccgaacgcg
     2041 ccgccgtcga tcagcccggc caccaccacg acggccacga acgccatgaa ccagacggcc
     2101 gcccgacggc gcgtgctgaa gagcagcgcc cccaccggcg aggtcagcga ccacaggatg
     2161 acgccgctgg agttgacgaa gccgcccagt acgagcatca gcagcgccgg aaagatcagc
     2221 gtgatgagca gctgcccgaa gcgaaacaga tgatagcggc cggtcagcgc gaaatagccg
     2281 atgctggcga acgagaacgc ggcataggcg atggggatgg ccccggcgtt gaccgcgccg
     2341 aagtacatat agatcaaccc ccaggtcatc gcgagcaggg cgatgacggt cgatgaggtg
     2401 acgagcagtt cggtttgcaa ctgttcgcgc tgtgcctggt cggtcagatg ggccgtcgat
     2461 tggacggcga gttgttgtgt ggtcatgttg acatcatccc ttgtcgcccg atcctctgtt
     2521 cgagcggcgc gccgccacac attgtagcaa aggaaggttc aggtgctgca tatcaatttg
     2581 gagctgcctg gccggcaacc agggaaaacc aaaaacgggc tttgggactg ctcccaaaac
     2641 ccgttttggt tgttatcagg ccggatttca cgaatgaaat ccggcctgag cggataaggt
     2701 tagttgccgc gactgttgac gcgaccggag tcgcaggcca acgcggtgcc cgcgaatacg
     2761 tcggaggtca tctccgcgca gttcgtccag gcaccggccc gcttgccgac agccgtgaag
     2821 gagatggtgt gggattcgcc cggagtggga ggcgtggtgg atacgagcac ctcgtagccg
     2881 cccgacgaag gcgagggagg ggcgccgttg ccgttgtagt agacggtgcc gccgcggctg
     2941 ccgaacgagt tctccgcgcc cacggtcaga tagccgccgt cgccgccggt gatggccgac
     3001 ccgtaggtga aggagatgtc ttcgtagggg gtgacatcac ccgagacacc gatccagacc
     3061 tggaaggtat tcttctgggc cgcgttggag tagttcggca cattcctcca ttcgacgacg
     3121 atccaggaac tgccgcctcc cgacaatatg ccggctcgaa taccgccgcc gacaggagcc
     3181 gtggcgacgt tcaggtcggt ccagaacgga gccaggacat tgttcggcgc agtggcattg
     3241 ggcaggttgg tgttgatgta cgtgacatcg gccgcggtcc caccaccgac aattgcgtag
     3301 ccgttggatg tgacgccgat tctggtgtag acctcaccgc catagagaaa cgccggaacg
     3361 ttgaagttgc cgatcgtttc atccccaaat ccggctacgg cggcgccacc gagcgcgtaa
     3421 tagcccgcgg gtgaggccag cggatcgacg gtcacattga catccggcgg ctcggcccct
     3481 tgcagcacac cgctcgcctg cacgccgttc ttgcggccga tctgcgtccc gccgacaacc
     3541 gtgccggcag tcagacggag ctgcttcggc agcatatcga tcaggttgaa cgaggcgtca
     3601 tcgaacccgg tgttggtcag ggtgatggag cagctggtgc tttcccggac gcgcaggacg
     3661 ctgggggtgc atgtcttgct catctcaacc acggcctggc cgcggacgat ggtgatgggg
     3721 aacgtgagcg acccgccgcg gttggaggtc atccgcagcg tcgcgtgccg aacctcgcct
     3781 tgcggcacgg tcggggccga tacggtgatc aggaacgagt aggtgccgtt ggccggaacg
     3841 ttacctgaaa tgcagaattc ccctcttgac atggcggaat tatccttgat aattttgata
     3901 gcgatctgta aaattgtttc gccacaggca agtatcctca tggaagcctg tttggtcatt
     3961 gttgattcga cgacgcgctg ctgtgccaac cggcgcggac cctcacatct caaggagaaa
     4021 ggaacatggt gtcaaagatt gggcggatcg tggtcgtaat cggattgatc ctggtcggaa
     4081 cgtggttgac gatcaggagc gcctcccccg cggccgaaag ctcggctcgt gtggcggcaa
     4141 ttcggcaagc cgcaccggct tcgcgcatcg gttcttcgag cgcgggccag gtgtcggata
     4201 tggttgttgc cgaacccaga acagtcgacc tgcggagtat tcccgcaggc gtctatgatc
     4261 ccgacaacca gtatgatcgc tggcagcgcg gcgagatcga cctgttggag aacgaaggca
     4321 ttttgtccgc ggccgaaatg gccaaactgc gcgcagactc ggaacggctg cccgcctcgg
     4381 gcaatatcac gctggcggac ggggccgacg gcatcggctc caacgctccc tcgcccgccg
     4441 gggccagctt cgacagcatt gactacaccg actgttgcgg cggcggcggc aatgtcccgc
     4501 ccgaccccga actggccgtc gggcctaatc atgtcatcgc tgtggtcaac gtggccctgg
     4561 agatttacaa caagaacggc aacagtcttg tcggcccaac gacctttgcc agcttcttca
     4621 actcggaccc caactgcacc ggcgttttcg accccaacgc cctctatgat gaagaggccg
     4681 accggtatat ggttggcatt gatgccgatg gcttctacta ttgcatcgga gtctcccaga
     4741 ccggcgaccc gaccggaaac tggaacatct actccttccc cacggcgagt ggcgccgact
     4801 tcttcgacta cccccacgcc ggggtaggcc gcgacgccat ctatatgggg gccaacattt
     4861 tcgataccgg tttcaaggaa agccgcgtct gggcgtttga caagtgggcc atgtacagcg
     4921 gcgcgtcggc cgccggagtc agcaagcctc tgccggcaac ggaagatacc ccgcaacccc
     4981 tgaaccttca cgggtggcag caaaacacct ggccgacgag cgggccgcat tacttcttca
     5041 ccgagaccga ctacaacggc gcgacctact ccgtctggtc gtggaccaac ccgttcggcg
     5101 gcacgtcccc cagcaaggtc ggcacggtca acctgacgac cttcaccggc gtgtcggccg
     5161 gaatgccggt cgccgtgccc cagaatggcg gccagacgat tcaaggcaac gattatcggc
     5221 cgcaggattt cgaatatcgc aacggctacg cctggagcat cagcaccgtc gcctgtaacc
     5281 ccggcggcgg cacggtcaac tgtattcgct gggcacagat caacccggcc acggcgacca
     5341 tcgtcgatgc cggcgtctat agcagctccg gggaatatcg cttctttggc gacctggctg
     5401 tgaaccattg cggcgatgtg gctgtgggct ataccaaatc gagcaccagc atgtggccgt
     5461 cggtcttcta cactggtcgc aaggcgggcg atgccgcggg cacgctgcag gccgaggcgc
     5521 agctgaagg
//
LOCUS       DAOMQG010000227         5496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6257_length_5496_cov_8.033634, whole genome shotgun sequence.
ACCESSION   DAOMQG010000227 DAOMQG010000000
VERSION     DAOMQG010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6257_length_5496_cov_8.033634"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..727
                     /locus_tag="PLC06_11675"
     CDS             38..727
                     /locus_tag="PLC06_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91149.1"
                     /translation="MKILLVEDDLALSDVVSFTLRRAGYVVVAAYDGLAALEQFAAAA
                     PDLLLLDLNLPRLDGLGVCRQVRAESDVPIIILSVRGEDEAVVRGLEMGADDYMVKPF
                     SPAQLVARIRAVMRRSGVAAAPSSLVAGGLTLDRSRGEVARAPDQAGQPPVRLTALEL
                     KLLEALMIDPGRVLPSDTLIAAVWGPEGGDRAMLKQLVYRLRAKIEDETTGGYIETVP
                     GIGYALARNDE"
     gene            1017..2000
                     /locus_tag="PLC06_11680"
     CDS             1017..2000
                     /locus_tag="PLC06_11680"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91150.1"
                     /translation="MTLTIDLNAFPTTTADTPAGSDLLRRVDRRVASYYLAVPLAGEG
                     DRVTVATAYPDNAGALRVLERLLQAAVVPVAGSEDELLAAIARIYPENVPGEAAIMAW
                     GDDPAEIESAVAAANAFGFAFGRPVVVMDTAATADAVIAHAGHDVSLLVARVADEAAR
                     ERLVRQSPVSLLLARGDYAPIARALVALRGYGSDHESLQRICPLLAREGSEATVLPLT
                     HPRSARPNGPLNGNAAARRHLQQFLRKLNGADTRVDIRLSQGDPADQIITELAGGRYD
                     LLVVAAEAEGDFVWRVLSRIEREDVWPGRPVMVVKPPVRITNYELRMTNDE"
     gene            2095..3075
                     /locus_tag="PLC06_11685"
     CDS             2095..3075
                     /locus_tag="PLC06_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRO91151.1"
                     /translation="MKLLVLMAVMGLTLALIACGGSSSAGSSASASEPAQGNPRTINV
                     SGAFALFPMMTVWAEQYSAATPNIRFDVQGGGAGKGMTDVLSGAVDIAMVSREIKPEE
                     TQQGAFGVPVTIDAVVPTANANNPHLTDLLATGLTPESAAAVWIGRTATTWGQFLGTD
                     ATEAITVYTRSDASGAGEVWAKFLGGTAQEDLTGTAVNGDPGLAEAVRQDALGIGYNN
                     IGFAYNLETGAQLDGLVVIPLDLNGDGTIGDDENFYETKDAIAAAIAGRTFPYPPARE
                     LYLVTKGEPDADIAAFYRWVLTEGQAMVADAGYVNLNEEQIAAALALLGE"
     gene            3110..4120
                     /gene="pstC"
                     /locus_tag="PLC06_11690"
     CDS             3110..4120
                     /gene="pstC"
                     /locus_tag="PLC06_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRO91152.1"
                     /translation="MEISRRDTVNLKTLETVVPLPAAPDFHRPRGQVRMIKSRVAKYV
                     LFAASVLLILLLVAIAGVLVYEALPILRQNSLIDILTSQAWHPTQLAFGLLPFIAGSF
                     IVTAFAMLLGVVPAVLSGIYLAEYTSSRRRSMVKPVVDLLAGIPSVVYGLWGVLFVVP
                     LIREVIAPALGGALGGRFPLFAVNNPSGYSVLAGAIVLAIMVFPLIVAVTDEVLRSIP
                     RQLRESTLALGATRWETTWCVVRHAGLPGIAAAVVLAFSRAFGETLAIMMVIGNTPII
                     PRSIFDAAYPLPALIANAYSEMMSIPLYQSALMLAALVLLCVVLFFNIIARRVIVRLG
                     KG"
     gene            4240..4617
                     /locus_tag="PLC06_11695"
     CDS             4240..4617
                     /locus_tag="PLC06_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRO91153.1"
                     /translation="MKKQVMKRYSEAFKRQVVGEYEEGHSVASLQAKYGITGNGTIER
                     WIKKYAHAGLRHELVIIQRGDERDRQKAVEARVRELETAVAQLTLEKIVLESSLAEAE
                     KLLGMEVKKNGAPPSSKPALNTR"
     gene            4614..5444
                     /locus_tag="PLC06_11700"
     CDS             4614..5444
                     /locus_tag="PLC06_11700"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRO91154.1"
                     /translation="MRQAHGFGKQDFLRQFGISRQAHRQQAAVLLRRAGEEAVILEMV
                     RAIRYHHPRMGTRKLLAKLRAQLVAREISLGRDALFDLLRRHDLLLSPKRRQTRTTRS
                     GLWRCPNLLPELTVTGPRQLWVGDITYLTHEGGFAYLALLTDAFSRFIVGFDLSVSLA
                     VEGCLRALNQALPQRPGSLAGLVHHSDHGVQYTAHTYRNRLAEHQIRSSMGEVGNCYE
                     NALAERMNGILKLEYGLDDRFVNLEQMTASTAQAVALYNYDRPHLALGYRVPAEVHFH
                     "
BASE COUNT          938 a         1714 c         1799 g         1045 t
ORIGIN      
        1 gttcgtcgtt cgcaaaatgc gctaaaatca ccacgccatg aaaattctcc tcgtcgaaga
       61 tgatctcgcc ctgtccgatg tcgtgtcgtt caccctgcgg cgggcggggt atgtggtcgt
      121 ggccgcctat gacgggttgg cggcgctgga gcagtttgcc gcggccgcgc ccgacctgct
      181 gctgctcgat ctgaacctgc cccggctcga cgggctgggc gtctgccgcc aggtgcgagc
      241 cgagagcgac gtgccgatca tcatcctcag cgtgcgcggc gaggacgagg cggtggtgcg
      301 cgggctggag atgggggccg acgactacat ggtcaagccg ttcagcccag cccagctcgt
      361 cgcccgcatc cgggccgtta tgcgccggtc gggcgtggcc gccgcgccgt cttcgttggt
      421 ggccggcggg ctgacgctcg atcggtcgcg gggcgaggtg gcccgcgcgc cggatcaggc
      481 cggccaaccg ccggtgcggc tgacggcgct ggagctaaag ctgctggagg cgttgatgat
      541 cgaccccggc cgggtgttgc cgtccgatac gctcatcgcc gccgtgtggg ggccggaggg
      601 gggcgaccgg gcgatgctga agcagctcgt ctatcgtctg cgagccaaga tcgaggacga
      661 aaccaccggg ggctatatcg agaccgtccc cggcatcggc tacgcgttgg ccaggaacga
      721 cgaatgacgg gtgggcgggc ggcggctgtc aatcgcacgg ccgccgcgag ttcggccgcc
      781 ccggccatca actccacgct cagttgccac aacgcattct gaccggcgcg ataatcggcc
      841 ggcatccgct cgcgccgctc tgtgcgggcg attttgaccg gttccgcgtc gtccgtccgg
      901 ggcacgaggg ccccacaccc tgcccctgat tcccgccccg ttgttaccat tttgttacca
      961 tcccgcgacc gcgtctgtta ccgcccccgg gtattcttga gaaatcaggt atatccatga
     1021 cgctaaccat cgatttgaac gcatttccga cgaccaccgc cgacaccccg gccggcagcg
     1081 acctgctgcg gcgtgtcgac cggcgcgtgg cgagctatta cctggctgtg ccgctggccg
     1141 gtgagggcga ccgcgtgacc gtcgccacgg cctaccccga caatgcgggt gcgctgcggg
     1201 tgctggagcg gctgctgcag gccgccgtcg tgcccgtagc cggttccgag gatgagttgc
     1261 tggcggccat tgcccggatt tacccggaga atgtccccgg cgaggcggcg atcatggcct
     1321 ggggcgatga tccggccgag atcgagtcgg ccgttgccgc ggccaacgcc tttggctttg
     1381 ccttcggccg tccggtagtc gtcatggata ccgccgccac cgccgacgcg gtcatcgccc
     1441 acgccgggca tgacgtttcg ttgctcgtgg cgcgggtggc cgacgaggcc gcgcgggagc
     1501 ggctggtgcg gcaatcgcct gtctcgctct tgctggcgcg cggcgactat gcgccgatcg
     1561 ctcgcgcatt ggtggcgctg cgcggctacg gctccgatca cgagtcgttg cagcgcatct
     1621 gcccgctgct ggcccgcgag ggaagcgagg ccacagtgct gcccctgacg catccgcgct
     1681 ccgcccggcc gaacggcccg ctgaacggca acgcggccgc ccgccgccat ctgcaacagt
     1741 tcctccgcaa actgaatggg gccgatacgc gggtggacat ccgcctgagt cagggcgacc
     1801 cggccgatca gatcatcacc gaactggccg gcgggcgcta tgacctgctc gtcgtggccg
     1861 ccgaggccga gggtgatttc gtctggcgcg tgctgtcgcg catcgagcgc gaggatgtct
     1921 ggccgggacg gccggtgatg gtggtgaaac caccggtcag aattacgaat tacgaattac
     1981 gaatgacgaa tgatgaataa tgagcagggg caggcctgtg tgtctgcccg gatacgagaa
     2041 ccgaaagaga cagaacaatt tttgaggaga tttgaaacag agatgatacg aacgatgaag
     2101 cttttagtat tgatggcggt aatgggtctg acccttgcgc ttattgcttg cggtgggagt
     2161 tcgtcggccg ggtcgtccgc gtcggcctca gagccggccc agggcaatcc gcgcaccatc
     2221 aacgtctccg gcgcgttcgc gctgttccca atgatgaccg tgtgggcgga gcagtactcg
     2281 gccgccactc ccaacatccg cttcgacgtg cagggcggcg gcgcgggcaa gggcatgacc
     2341 gatgtgttgt ccggggcggt ggatatcgcc atggtctccc gtgagatcaa gccggaagag
     2401 acgcagcagg gcgcgtttgg cgtgccggtc accatcgacg ccgtggtgcc gacggccaac
     2461 gccaacaacc cccatttgac cgacctgctg gctacaggtc tgacgccgga atcggctgcg
     2521 gccgtctgga tcggccgaac cgcgaccacc tggggccagt tcctgggcac ggacgcaacc
     2581 gaggctatta ccgtctatac ccgttccgac gcctcgggcg cgggcgaggt gtgggccaaa
     2641 ttcctgggcg gcacggcgca ggaagacctg acgggcacgg ccgtcaacgg cgaccccggt
     2701 ctggccgagg ctgtccggca ggatgcgctg ggcatcggct ataacaatat cggcttcgcc
     2761 tataacctgg agaccggcgc acaactggac gggctggtcg tcatcccgct ggacctgaac
     2821 ggcgacggaa cgatcggcga cgatgagaac ttctacgaga cgaaggacgc catcgccgcg
     2881 gccatcgccg gccgcacctt cccttatccg cccgcccgcg agctttatct cgtgaccaag
     2941 ggcgagccgg atgccgacat cgccgccttc taccgctggg tgctgaccga ggggcaggcg
     3001 atggtggccg acgcgggcta tgtgaatctg aacgaagagc agatcgccgc cgctctggcc
     3061 ctgctgggcg agtgaaacag aacggccacg atttggcgag gaggatctca tggaaatctc
     3121 aagacgtgac acggtcaacc tgaaaacgct cgaaacggtc gtgccgctgc cggccgcgcc
     3181 cgatttccat cgcccccgcg ggcaagtgcg gatgatcaag agccgggtcg ccaagtacgt
     3241 cctgtttgcc gcctcggtac tgctcatcct gttgctggtg gctatcgccg gggtgctggt
     3301 gtatgaggcg ctgcccatcc tgcgccaaaa ctccctgatc gacatcctga cctcgcaggc
     3361 gtggcacccg acgcaattgg cgttcggctt gctgcctttt atcgccggga gcttcatcgt
     3421 caccgccttc gccatgctgc tgggagtggt gccggcggtg ctgagcggca tctacctggc
     3481 cgagtacacg tcatcgcggc ggcggtcgat ggtcaagccg gttgtcgatc tgctggcggg
     3541 tattccgtcg gtggtctatg ggctgtgggg cgtgctgttc gtcgtgccgc tcatccgcga
     3601 ggtgatcgcc ccggcactgg gcggcgcgct gggcgggcgc ttccccctgt tcgcggtcaa
     3661 caatcccagc ggctacagcg tgctggccgg cgcaatcgtg ctggcgatca tggtctttcc
     3721 gctcatcgtg gccgtgaccg acgaggtgtt gcgcagcatc ccgcgccagt tgcgcgaatc
     3781 aacgctggcg ctgggggcca cgcgttggga gacgacatgg tgtgttgtgc gccatgccgg
     3841 gctgccgggc atcgccgcgg ccgtggtgct ggccttctcg cgcgcgttcg gcgagacgct
     3901 ggccatcatg atggttatcg gcaacacgcc cattatcccg cgctccatct tcgacgcggc
     3961 ctacccgctc ccggcgctca tcgccaacgc ctatagcgag atgatgtcca tcccgctcta
     4021 tcagtcggcg ttgatgctgg cggcactggt gctgctgtgt gtggtgttgt tcttcaacat
     4081 catcgcccgg cgggtgatcg tgcggctggg gaagggatga attgcgaatt acgaattgcg
     4141 aattacgaat tacgaattac gaattacgaa ttgtgacccg tcctaaataa gattgacatc
     4201 aggtatccta taactgactc gataccaagg atcgacctga tgaaaaaaca agtcatgaaa
     4261 cgatacagcg aagcgttcaa gcggcaagtt gtcggcgagt atgaggaagg tcatagcgtt
     4321 gcctccttgc aggccaaata cggcatcacc gggaacggga caatcgagcg ttggatcaag
     4381 aagtatgccc acgctggatt gcgccacgag ttggtcatca tccagcgcgg cgatgagcgc
     4441 gaccggcaga aggcggttga agcgcgcgtg cgggagctgg aaacagccgt ggcccagttg
     4501 acgctggaga agatcgtgct ggagtcctcg ttggccgaag ccgagaagct gctggggatg
     4561 gaagttaaaa aaaacggcgc gccgccatcc tcgaagcctg ctttgaacac ccgttgaggc
     4621 aagcccatgg ttttggtaaa caggacttcc tgcgacagtt cggtatcagc cgtcaggctc
     4681 accggcaaca ggctgcggtg ctgctgcgac gggcgggcga ggaggccgtg attctggaaa
     4741 tggttcgggc gattcgctac catcatccgc gaatgggcac gcgcaaattg ctggccaaat
     4801 tgcgcgctca actggttgcc agagagattt ctctgggtcg tgacgcgctt tttgacctgc
     4861 tccgtcgcca tgacctgctg ctgtcgccca aaaggcgcca gacgagaacg acgcgctccg
     4921 gcctctggcg ttgccccaac ttgctgcctg agctgacggt gaccggcccg cgccagcttt
     4981 gggtaggcga tatcacctac ttgacgcatg agggcggctt tgcctacctc gctctcctga
     5041 ccgatgcctt ttcgcgattc atcgtcggct ttgacctgtc cgtttccctc gccgttgaag
     5101 gctgtctgcg tgctctcaac caagccttgc cgcagcgtcc cggctcgctg gccggtctcg
     5161 ttcatcatag tgaccacggc gtgcaataca ccgcccacac ctaccgcaac cgtctggccg
     5221 agcaccagat tcgctccagc atgggcgagg tcggcaactg ctacgaaaat gccctcgctg
     5281 aacgcatgaa cggcatcctg aaactggagt atggattgga cgaccggttc gtcaacctgg
     5341 aacagatgac ggcctcgacc gcccaggccg tcgcgcttta caactacgac cggcctcatt
     5401 tggccctggg ttatcgtgtc ccggcggagg tgcattttca ttaacataac aatcagtgtt
     5461 cagtgttctg atgtcaatct attttaggac ttgaca
//
LOCUS       DAOMQG010000228         5492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6263_length_5492_cov_9.536141, whole genome shotgun sequence.
ACCESSION   DAOMQG010000228 DAOMQG010000000
VERSION     DAOMQG010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6263_length_5492_cov_9.536141"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..3149
                     /gene="gcvT"
                     /locus_tag="PLC06_11705"
     CDS             18..3149
                     /gene="gcvT"
                     /locus_tag="PLC06_11705"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRO91155.1"
                     /translation="MSDDFIFRGSLAEVDEAVEAIIEREDARQASTIILIASESEAPE
                     AVVEALGSTLGNIYAEGYPREESRRQTEAEITDIDMELAHYRRYSDPRYYKGVENADI
                     IEALARRRAAELFAANGISPDNLYVNVQPLSGAPANSAVYTALLQPGDTILGLNLNDG
                     GHLTHGSPVNRSGKVYKGVPYFVDPETEELDYDAIERTALEARPQIIVAGYSAYPRII
                     DWKRFREIADKVGAYFLADIAHISGLVAAGVHPSPIGIADVVSTTTHKSLCGPRGAML
                     MTHRRDLARKLDRAVFPGEQGGPHMNTIAALAVALKLAKTDRFHALQQRIVDNASRLA
                     QQLQAHGIRVIGGGSQNHLLLIDTKSVSVDGVHLTGDMGSRILDVAGIVVNRNTIPGD
                     KGALSPTGIRIGTVWISQLGFGPAEIDLLAEAIATVLKGARPYLRQGAGGKRELRARV
                     DYRALKRGREIVRQLRKVPAAAAGPDVVVRGPEAATFLNYALTSDVAALADGEAQVTH
                     LFGPDMSLDATLQRVTAERFMLHFEREEAAAEAAEWLQALSDGYAQFDDVYARLPGPI
                     VAQIVPAGIGDAIGNAIARAATALRNDDKDDSARYADSKPYFVGRERRPTSGSPLPPF
                     VWEEPVDPPLLATNLHETHKALGARMVPFAGYDMPVWYTSVSEEHAAVRETAGLFDVS
                     HMGVLDASGPYALEFLEAVTGNDVSTLAVGSSQYSQFLMPDGSVIDDLMVYRVADDSY
                     LVVVNASNNDKDWAWLNAVNEGRVLIDPDRPWARLQHPAVLRDLRDPQHGADCRVDLA
                     LQGPRSADILNALAGNDPAFARRLKALPWAGVLRANPGGFDLIISRTGYTGERIAYEL
                     FIHPDRAVELWNALMAAGEPFGMKPCGLAARDSTRTEAGLPLYGHEMAGAQNLNPADA
                     GFGSFVKMYKPFFIGRRAFIDHEEKRDSVVVRFRMNEKGVRRPETGDPVLDRRGKVVG
                     HVTSCAIDGEGYLLGQAIIPLSMSEPGTPLSIYQMGGGTRPIKAADKVSLGSRLPVPD
                     AAVVLTRFPERKK"
     gene            3209..3709
                     /locus_tag="PLC06_11710"
     CDS             3209..3709
                     /locus_tag="PLC06_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRO91156.1"
                     /translation="MRREVLTWSDVDKLIDYLVPQIRGRFDSMVIITRGGIVPGGMLA
                     EALDITYILTASVRFPSDFPGMQPEMVKYAIPEFIQFPDEEILRGRRVLVVDDVWTKG
                     RNSVTVANRIDAAGGIPEICVLHYKPSYSLYPGYAPTYYAAVSDAYIIYPWELDRGPE
                     VLGVWN"
     gene            3900..3998
                     /gene="ffs"
                     /locus_tag="PLC06_11715"
     ncRNA           3900..3998
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLC06_11715"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4943..>5492
                     /locus_tag="PLC06_11720"
     CDS             4943..>5492
                     /locus_tag="PLC06_11720"
                     /inference="COORDINATES: protein motif:HMM:NF015923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HRO91157.1"
                     /translation="MHQRIRPGLFVVLWALSAVACRPAELTYTVRDGATTVPVTGRYE
                     TTGDVLAAAGLSPAEEDTVAPGLNAPADPATPIVIDRAAEVTVITPDSVTTWRTQAQT
                     LGAFLAEAGLAPPPGSRLLADGKPLAIEDAANTPLPADIIFDPWKRVIVTDGNQRRAL
                     RTTALTVGEALAEAEIALGALDN"
BASE COUNT          980 a         1827 c         1713 g          972 t
ORIGIN      
        1 tcggtggagg tacaacaatg agtgacgatt ttatctttcg cgggtctttg gccgaggtcg
       61 acgaggccgt agaggcgatc atcgagcgcg aagacgcccg acaggcgtcg acgatcatcc
      121 tgatcgccag cgaaagcgaa gcgcctgagg cagttgtcga ggcgctgggc agcacgctgg
      181 gcaatatcta cgccgaaggc tacccgcgcg aggagagccg ccgccagacc gaagccgaaa
      241 tcaccgacat cgacatggaa ctggcccact atcgccgcta cagcgacccg cgctactaca
      301 agggcgtgga gaatgccgac atcatcgagg cattggcccg ccgccgcgcg gccgagctgt
      361 tcgccgccaa cggcatcagc cccgacaacc tctacgtcaa cgtccagccg ctgagcggcg
      421 cgccggccaa cagcgccgtc tacaccgcgc tgctgcagcc gggcgacacc atcctgggcc
      481 tcaacctcaa cgacggcggc catctgaccc atggctcgcc cgtcaatcgc tccggcaagg
      541 tttacaaggg cgtgccctac ttcgttgacc cggagaccga ggagctggac tacgacgcca
      601 tcgagcgcac ggcgctggaa gccaggccgc aaatcatcgt cgccggctat tcggcctatc
      661 cgcgcatcat cgactggaag cgcttccggg agatcgccga caaggtgggc gcctacttcc
      721 tggccgacat cgcccacatc tccggcctgg tggccgcggg cgtccatccc agccccatcg
      781 gcatcgccga cgtcgtctcc accaccactc acaaaagcct gtgcgggccg cgcggggcca
      841 tgctgatgac ccaccggcgc gatctggccc gcaagctcga ccgcgccgtc ttccccggcg
      901 aacagggcgg cccgcatatg aacaccatcg ccgcgctggc cgtggccctg aagctggcca
      961 aaaccgaccg gttccacgcc ctgcaacagc gcatcgtcga taacgcgagc cggctggcgc
     1021 aacaactcca ggcgcacggc attcgcgtca tcggcggcgg gtcgcagaac cacctgctgc
     1081 tcatcgacac caagagcgtc agcgtggatg gggtgcatct gaccggcgac atgggttcgc
     1141 gcatcctcga cgtggccggc atcgtcgtca accgcaacac catccccggc gacaagggcg
     1201 cgctctcgcc caccggcatc cgcatcggca cggtgtggat cagccagttg ggcttcggcc
     1261 cggcggagat cgatctgctg gccgaggcca tcgctaccgt gctgaagggc gcgcggccgt
     1321 acctgcgcca aggcgcgggc ggcaagcgtg aattgcgggc gcgggtcgat taccgggcgc
     1381 tgaagcgcgg ccgggagatc gttcgccaat tgcgcaaggt accggccgcg gccgccgggc
     1441 cggatgtggt cgttcgcggc ccggaagcag caaccttcct caactatgcc ctgacgagcg
     1501 atgtggccgc gctggccgac ggagaggccc aggtgaccca tctgttcggg ccggacatga
     1561 gtctggacgc cacactacag cgcgtgaccg cggagcgctt catgctccac ttcgagcgcg
     1621 aagaggccgc ggccgaggcc gccgagtggc tgcaggcgct gtcggacggc tacgcccagt
     1681 tcgatgacgt ttatgcccgc ctgcccgggc cgatcgtggc ccagatcgtg cccgccggta
     1741 tcggcgatgc gatcggcaac gccatcgcgc gcgccgcgac cgccctgcgc aacgacgaca
     1801 aggacgacag cgcgcgctat gccgacagca agccctattt cgtcggccgc gagcgccgcc
     1861 cgacttccgg ctccccgctg ccgcctttcg tctgggaaga gccggtcgat ccgccgctgc
     1921 tggcgaccaa cctgcacgag acccacaagg cgctgggcgc gcgcatggtg ccctttgccg
     1981 gctacgacat gccggtctgg tacaccagcg tcagcgagga acacgccgcc gtgcgcgaga
     2041 cggccggcct gttcgatgtt tcccacatgg gcgtcctgga cgcgagcggc ccctacgcgc
     2101 tggaatttct ggaggcggtg acgggcaacg acgtctcgac gctggccgtc ggttcgtcgc
     2161 aatatagtca attcctgatg cccgacggct cggtgatcga tgacctgatg gtctatcgcg
     2221 tcgcggacga cagctatctg gtcgtcgtca acgcctcgaa caacgacaag gactgggcct
     2281 ggctcaatgc cgtcaacgag gggcgcgtgt tgatcgaccc cgaccggccc tgggcacggc
     2341 tgcagcaccc cgccgtcctg cgcgacctgc gcgacccgca acacggcgcc gattgccggg
     2401 tggacctcgc cttgcaaggc ccgcgctcgg ccgacatcct caacgcgctg gcgggcaatg
     2461 acccggcctt cgccaggcgg ctgaaggcat tgccctgggc gggcgtcctg cgcgccaacc
     2521 ccggcggctt cgatctgatc atctcgcgca ccggctacac cggcgaacgg atcgcctatg
     2581 agttgttcat ccaccccgac cgcgccgtgg aactctggaa cgcgctgatg gccgcgggcg
     2641 agccgttcgg catgaagccg tgcgggctgg ccgcgcgcga cagcacgcgg acggaagcgg
     2701 gcttgccgct ctatggccac gagatggccg gggcgcagaa cctgaacccg gccgacgcgg
     2761 gcttcggcag cttcgtcaag atgtacaagc cgttcttcat cggccgccgc gccttcatcg
     2821 atcacgaaga aaagcgcgac agcgtggtgg ttcgcttccg catgaacgag aagggcgtgc
     2881 gccggccgga gacgggcgac ccggtgctgg atcgtcgcgg caaggtggtg gggcatgtga
     2941 ccagctgcgc catcgacggc gagggctatc tgctgggcca ggccatcatc ccgctgagca
     3001 tgagcgagcc gggcacgccg ctcagcatct accagatggg tggcggcacg cgaccgatca
     3061 aggccgccga caaggttagc ctcggcagcc gcctgccggt gcccgacgcg gccgtggtgc
     3121 tgacgcgctt cccggagcgg aagaagtagt tcaattgaca tgatcccggc aaggcgatag
     3181 gatcgtcggg ctgaatgagg agtggaacat gcgacgcgaa gtattaacgt ggtctgacgt
     3241 cgacaaactg attgattatc tcgtgccgca gattcgcggg cggttcgatt cgatggtgat
     3301 catcacccgt ggcggcatcg tgcccggcgg catgctggcc gaggcgctcg atatcaccta
     3361 catcctgacc gcctcggtgc gctttccgtc cgactttccg gggatgcagc cggagatggt
     3421 gaagtacgct atcccggagt tcatccagtt cccggatgag gagatattac gcggccgccg
     3481 cgtgctggtc gtcgatgacg tatggaccaa ggggcgcaac agtgtgacgg tcgccaaccg
     3541 catcgacgcc gccgggggca tcccggagat ctgcgtgctg cattacaagc cgtcctactc
     3601 gctctatccc ggctacgcgc ctacctatta cgccgccgtc agcgacgcct acatcatcta
     3661 cccgtgggag ctggatcgcg ggccggaagt gctgggagtc tggaattaat tacgaattac
     3721 gaattacgaa ttacgaatca ggaatgaaga aatgaatgcg ttgcggtgag gcgttcctga
     3781 ttcgtaattc ctgattgaaa tgagcgccgc gcaagatggg gccgcccgag cgttgcccaa
     3841 cgagcggcga cgggctatca ttggcccctt aacagaaatg cgcccatgaa cccggtgacc
     3901 actgggtccc tggcggcgga agccgtcgaa ccgtgtcagg tccggaagga agcagcacta
     3961 agatgtctct tccgggtgcc caggacaacc tggctggtca tcgggttcat gggcgcattt
     4021 tgtctctact ccccaaaaca gagacctgac aggtgggcgc gtggagttgt tggtttgacc
     4081 gggtcgttcg tgcgcccacc tgtcaggtct gggtgtatcg ttcacaggat gccttcgtgg
     4141 ggggtggcag ccttgtggtt agtgatattc tggaagccgc cttgctccag ccggcgcagc
     4201 gccgggttgt gggcgagacc tgacaggtgg gcgcgcggag ttgttggttt gaccgggccg
     4261 ttcgtgcgcc cacctgtcag gtctgggtgt atcgttcaca ggatgccttc gtggggggtg
     4321 ggcttgtgac tagtgaggct atgttgacta gtgagggcat tatcacgagt gataatgttt
     4381 aatccggtta tttttgatgg atcgagtcca tagctttctg cccaccttat taattctttt
     4441 aatgataaag accatccata tttccccgtc ccttttgggc gctccttttt cagcctcatt
     4501 tttgacaata tcctccctac cttacttgat gtaatatgat caagttggat atctatctcc
     4561 tgaacatgag ctattgcttg cgcggattgt gtgattgtgg ctgtaagaag gtgatacctc
     4621 ttgttgtatc cctcactagc ctcactgacc tcactagtca caaattcacc tgatcctatt
     4681 cggcccaaca agaaccccaa cacccaaatc accagcgccg tcggacgacg ggagcagggg
     4741 agcaggggtg aaagggagca ggggtgcggg ggagcagagg cgctctgatc tccccctctc
     4801 cctatctccc cctctccccc tctccctctc tccctctctc cccttctccc cttctccctt
     4861 tctcctttcc gatcattaca attccgatca tatttaccgc cgcgccttga aaaaacggcg
     4921 cgtattccct atccttggcc ccatgcacca gcgcatccga cccggcctct tcgtcgtcct
     4981 ttgggcatta tcggccgtcg cctgccgccc ggcggagttg acctacaccg ttcgggacgg
     5041 agcgacgacc gtccctgtaa ccggccgata tgagaccacc ggcgatgtgc tggccgccgc
     5101 cgggctgtcg ccggccgaag aagacaccgt cgcgcccggc ctcaatgccc ccgccgaccc
     5161 ggccaccccc atcgtcatcg accgcgccgc cgaggtgacg gtcatcaccc ctgacagcgt
     5221 gacgacctgg cgcacccagg cgcaaacgct gggcgcgttc ctggccgagg ccgggctggc
     5281 cccaccgccg gggtcgcggc tgctggccga cggcaagcct ctcgccatcg aggacgcagc
     5341 caacacacca ctcccggccg acatcatctt cgacccctgg aagcgcgtga tcgtgaccga
     5401 cggcaaccag cgccgcgccc tgcgcacaac cgccctaacc gtcggcgagg cgctggccga
     5461 ggcagagatc gccctgggcg cgctcgacaa cg
//
LOCUS       DAOMQG010000229         5480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6284_length_5480_cov_8.411429, whole genome shotgun sequence.
ACCESSION   DAOMQG010000229 DAOMQG010000000
VERSION     DAOMQG010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6284_length_5480_cov_8.411429"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="moeB"
                     /locus_tag="PLC06_11725"
     CDS             complement(<1..806)
                     /gene="moeB"
                     /locus_tag="PLC06_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRO91158.1"
                     /translation="MTHPSLIDPAALADINLSHEEIRRYSRHLIMPEVGMSGQKKLKA
                     ASVLLIGAGGLGSPLAMYLAAAGVGRIGLVDYDTVDFSNLHRQIIHGTKDVGRLKLES
                     AKDRILDINPNVQVDTYEVPLTSANALELFAPYDIIIDGTDNFPTRYLTNDACVLLGK
                     PNVYGSIFRFEGQASVFYAKEGPCYRCLFPEPPPPGLVPSCAEGGVLGVLPGTIGAIQ
                     TTEAIKLILGVGEPLIGRLLLYDATTMTFDEVRLRKNPNCPICGEHPTIT"
     gene            complement(822..1295)
                     /locus_tag="PLC06_11730"
     CDS             complement(822..1295)
                     /locus_tag="PLC06_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRO91159.1"
                     /translation="MIERVALLLILSLAAMIACYLLRAFHMRRIRASAPHPTAANTPA
                     LLYFRADSCAVCPAQGRIIDQLGAHWHDRVRMEIIDAEREPETAARYHVFTLPTTILH
                     DADGRVRQVNYGLTDERKLNRQLTELTGGHPADSEAARLPSDRGPSGIQSAQSAN"
     assembly_gap    1321..1420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1612..1884)
                     /locus_tag="PLC06_11735"
     CDS             complement(1612..1884)
                     /locus_tag="PLC06_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRO91160.1"
                     /translation="MTTIRIPTPLRPYSGGNSTVTVSGDTVGEALDHLTQQHPQLRNH
                     LFDGEELRSFVNIYLNKEDVRGLQGAQTALKSDDTLMIIPSIAGGM"
     gene            complement(1897..2337)
                     /locus_tag="PLC06_11740"
     CDS             complement(1897..2337)
                     /locus_tag="PLC06_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRO91161.1"
                     /translation="MLILPHPIHAQIAAHGAASYPYEGCGLLLGSAEDGRNIVVAAVP
                     LPNVWPVESEKPERFRIAEDDWRDVELEAMSNGLDVIGIFHSHPDHPAVASPRDLAWA
                     SWPGYSYLITQIIGGEPADGRSWQLMPDRSGFVEEPVEVVEDNQ"
     gene            complement(2378..3343)
                     /locus_tag="PLC06_11745"
     CDS             complement(2378..3343)
                     /locus_tag="PLC06_11745"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRO91162.1"
                     /translation="MIATIDNLELAVRADSSTQLEQLIGNTPLLRLDAAAAAKQLLNR
                     RVSSGVMLLAKAEWYNPGGSIKDRAALSIIRAAERRGLLRPGMTLLDATSGNTGIAYA
                     MLGAARGYRVRLAVPENVSPERLAILRAYGAELTLTDPLDGSDGAILEARRIAAADPT
                     AFYADQYNNPANWQAHYQSTAAEIWRQTDGRITHFVAGVGTSGTFVGTTRRLRELNPA
                     ILCYSAQPDSPFNGLEGWKHMATAIVPGIYDDTLADNELTARTEDAYATARYLARREG
                     ILVGVSAAANVHAALRVATELETGVVVTILPDSGYKYLSERFWQG"
     gene            complement(3649..4284)
                     /locus_tag="PLC06_11750"
     CDS             complement(3649..4284)
                     /locus_tag="PLC06_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91163.1"
                     /translation="MNEQKKPLQPPNPETVDAAMAAAYVSNRLTRGEDALYLARRRAA
                     QGDLEAAAGANDLLQGADMLAASEDIDSMTAVVHSLNEEDLDDAMEIGSISGELAVVG
                     DVLASLDMTTVSEFLLDRSQRLRELAVDNIMRYGALRALAHTLGETGEKVAELGAAEV
                     AEGAARMDLADAVTANSEAMAIAGEEMIAEGLATLAAAQGALDAGSELDAE"
     gene            complement(4363..5121)
                     /locus_tag="PLC06_11755"
     CDS             complement(4363..5121)
                     /locus_tag="PLC06_11755"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HRO91164.1"
                     /translation="MFQSLSNPAQRYWLIGLLILWAVLLFGGFLFGGPAEASRRMPAW
                     TRMASSATLVVAAFSWYAFTRDTPAGGYALLIAIGMTFGLLGDLALAGWLPGGRTVIM
                     GIAFFGVGHIFYIIAMLRFASAAGLTASGPRWGALAVWLLVGLVGWYIVVYRGASAAG
                     ITLTLVHWIALPYALLLAGTAGVATGLALQDGRFILLALGGALFLLSDLILAGEMFSG
                     MSFRLIGDVIWLTYGPAQMLIVYSISAVLSRLSR"
BASE COUNT          979 a         1568 c         1823 g         1010 t
ORIGIN      
        1 tcggtgatcg tcggatgctc gccgcagatg gggcagttgg ggttcttgcg cagcctcacc
       61 tcgtcgaagg tcatcgtcgt ggcatcatag agcagcaggc ggccgatgag cggctcgccc
      121 acgccgagga tgagcttgat cgcctcggtg gtctggatcg cgccgatagt gcccggcagc
      181 acgcccagca cgccgccctc ggcgcaactg ggcacgagac cgggcggcgg tggttcgggg
      241 aaaaggcagc gatagcacgg cccttccttc gcatagaaga cagacgcctg accctcaaag
      301 cggaagatgc tgccgtagac gttaggcttg cccaatagca cgcaggcgtc gttggtcaga
      361 tagcgcgtgg ggaagttgtc ggtgccgtcg atgatgatgt cgtagggcgc gaacagctcc
      421 agcgcgttgg ccgaggtcag cggaacctcg taggtgtcca cctgcacgtt ggggttgatg
      481 tcgaggatgc gatccttggc actctccagc tttaggcggc cgacgtcttt ggtgccgtgg
      541 atgatctggc ggtgcaggtt ggagaagtcc accgtgtcat agtcgacgag gccgatgcgg
      601 ccgacgccgg ccgcggccag atacatcgcc agcggcgacc ccaggccgcc cgcgccgatg
      661 agcagcacgc tcgccgcctt gagctttttc tgcccgctca tgccgacttc gggcatgatc
      721 aggtggcgac tgtagcgccg aatctcctcg tgggagaggt tgatgtcggc caatgccgcc
      781 gggtcaatca gtgatgggtg tgtcatagtg aatccagggt tctaattcgc ggattgcgca
      841 gattgtatac cggatgggcc gcgatccgaa gggaggcgcg ccgcctcgct atcagccgga
      901 tgcccgccgg tgagttcggt taactgccgg ttcagtttgc gttcgtcggt caggccgtaa
      961 ttgacctgcc ggacgcggcc gtcggcgtcg tggaggatgg tcgtcggcaa tgtgaagacg
     1021 tgataacggg cggccgtctc cggttcgcgt tcggcgtcga tgatctccat gcgcacgcgg
     1081 tcatgccagt gcgcgcccag ttgatcgatg atgcgccctt gagccgggca gacggcgcat
     1141 gaatcggcgc ggaaatagag cagcgccggt gtgttcgccg ccgtcgggtg gggggcgctc
     1201 gcccgtatcc ggcgcatatg gaaggcgcgc agcagatagc aggcgatcat ggccgccagg
     1261 cttaggatta gcagtaaggc gacgcgctca atcatggcgt taccgaccct gtttcatggg
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccacgaaga ccaccagcca
     1441 caccgagttg aaaacgaatg ccagcgccag cagggcgatg atcaatgttt gattgacctt
     1501 cagcgcggaa tggtcgactt ttggggacgt tgtttccatg aatggcctcg caaatgagtt
     1561 gatgatcgga cgttattgtc tgatgaccgg ttggtgcaat gcggtttcct cttacatccc
     1621 gccggcgatg gatgggataa tcatcaaggt atcatccgat ttcagcgccg tctgcgcccc
     1681 ttgcaggccg cgcacgtctt ccttgttcag gtagatgttg acgaagctgc gcaattcttc
     1741 gccgtcgaac agatggttgc gcagttgcgg gtgttgttgg gtgaggtgat ccagcgcctc
     1801 gccgacggtg tcgccgctga cggtgacggt gctgttgccg ccggaatagg gacgcaatgg
     1861 ggtggggata cgtatggttg tcatgaattt actcctctat tgattgtctt ccacgacctc
     1921 gaccggctcc tcgacgaaac cggaacggtc gggcataagt tgccatgaac ggccgtcggc
     1981 cggctcgccg ccgataattt gggtgatcaa gtacgaatag cccggccagc tggcccaggc
     2041 caggtcgcga ggcgaggcca cagccgggtg gtcggggtgg ctgtggaaga tgccgattac
     2101 gtccaggccg ttgctcatcg cctccagttc gacgtcgcgc cagtcgtcct cggcgatgcg
     2161 gaagcgctcc ggcttttcgc tttcgactgg ccagacgttg ggcagaggca cggcggcgac
     2221 gacgatattt cggccgtctt cggccgagcc gagcagcagc ccgcaccctt catacggata
     2281 cgaagccgcg ccatgggcgg cgatttgggc gtggatgggg tggggaagga ttagcataga
     2341 tttacttctg cggttgccgg cggccggtgg ttgtcggtca tccttgccaa aacctctcgc
     2401 tcagatattt atacccgcta tcgggcaata tggtcaccac gacgcctgtt tcaagttcgg
     2461 tggccacgcg cagggcggca tggacattgg ccgcggccga gacgccgacg agtataccct
     2521 cgcgacgggc cagatagcgg gccgtggcat aggcgtcctc ggtgcgggca gttagctcgt
     2581 tgtcggccag cgtgtcgtcg tagatgccgg gcacaatggc cgtcgccatg tgcttccagc
     2641 cctcaaggcc gttgaacggg ctgtccggtt gggccgaata gcacaggatg gcgggattga
     2701 gttcgcgcag gcgacgggtt gtgcccacaa atgtgccgct ggtgccgacg ccggcgacga
     2761 aatgggtgat gcggccgtcg gtctgtcgcc agatttcagc ggccgtgctc tgataatgag
     2821 cttgccagtt ggccgggttg ttgtattgat cggcgtagaa ggcggtcggg tcggcggcgg
     2881 caatgcgtcg cgcttcgagg atcgctccgt cggagccgtc cagtgggtcg gtcagggtga
     2941 gttccgcgcc gtaggcacgc aagatggcca gccgctcggg gctgacgttc tccggcacag
     3001 ccaggcgcac gcggtagccg cgggccgccc cgagcatggc gtaggcaatg ccggtattgc
     3061 ctgatgtggc gtcgagcaag gtcatgcccg gccggagcag ccctcggcgt tcggcggcgc
     3121 ggatgatgct cagcgcagct ctatccttga tggagccgcc ggggttgtac cattcggctt
     3181 tcgccagcag catgaccccg gaagagacac ggcgattgag caattgcttg gccgcagccg
     3241 cagcatcgag gcgtagcagg ggtgtgttgc ctatgagttg ttcaagctgt gtggaagaat
     3301 cggcacgaac ggccaactcc aggttatcga ttgtcgcaat catgatgcaa atgtctttat
     3361 ccgatccgat gtcgtctgtt gacggccaag gcgtcgagca ggcacaaaaa aacggtcgca
     3421 acttcccttg tgaagcgcgt gtaacagccg ggttacgcgc gagggcagtt accaccgtat
     3481 gctcgacagt ccgaacgccg gaccgggcct gcggtggctt ctacccccgc ttaagacatc
     3541 tgcaatattg ccgaatcatt tcttgaccta atcgttatta tcagaaggtt gattaaaagt
     3601 tctgctggcc ggaagtatat gaaatcgccg gaagcaagtc aagagccgtt attccgcgtc
     3661 cagctcgctg ccggcgtcca gcgcgccctg tgccgcggcc agggtcgcca gcccctcggc
     3721 gatcatctct tccccggcga tggccatggc ctcgctgtta gccgttactg cgtcggccag
     3781 atccatacgc gcggcgccct cggcgacttc ggccgcgccc agctcggcca ctttctcgcc
     3841 cgtttcgccc agcgtgtggg ccagggcgcg caatgcccca tagcgcatga tgttgtcgac
     3901 ggccagttcg cgcaatcgct ggctgcgatc gagcagaaac tccgacacag tggtcatgtc
     3961 cagcgaggcc agcacgtcgc cgacgacggc caactcgccg gagatggagc cgatctccat
     4021 cgcatcatcg aggtcttcct cgttcagcga gtgaacgact gcggtcattg agtcgatatc
     4081 ctcggacgcg gccagcatgt ccgcgccctg cagcaggtcg ttggcccccg cggccgcttc
     4141 cagatcgcct tgagcggcgc gccggcgggc cagatagagc gcatcctcgc cgcgggtcag
     4201 gcggttggag acgtaggcgg cggccatggc cgcgtcgacc gtctccgggt tgggtggttg
     4261 cagcggtttc ttttgctcgt tcatgatctt tctcccgcga attccagagt gacgattgcg
     4321 caagccggat tatatgtggt tgcgtgccgg gttacaatca gttcatcggc tcagccggct
     4381 caggacggcg ctgatggagt agacgatgag catctgggcg gggccatagg tgagccagat
     4441 gacgtcgccg atgagccgga agctcatgcc gctgaacatc tcaccggcca ggatgaggtc
     4501 actcagcagg aagagcgcgc cgccaagcgc caagaggatg aagcggccgt cctggagagc
     4561 caggccggtg gccacgccgg ccgtgccggc cagcagcagg gcgtagggca gggcgatcca
     4621 gtggacgagg gtgagggtga ttccggcggc cgaggctccg cgatagacga cgatatacca
     4681 gccgacgaga ccgacgagca gccagacggc cagcgctccc cagcgtggcc ccgacgccgt
     4741 tagaccggcg gccgaggcga agcgcagcat ggctatgatg tagaagatgt ggccgacgcc
     4801 gaagaaggcg atgcccatga tgacggtgcg gccgccgggc agccacccgg ccagcgccag
     4861 gtcgcccaga aggccaaaag tcatgccgat ggcgatgagc agggcgtagc cgccggcggg
     4921 agtgtcgcgc gtgaaggcgt accagctgaa cgcggcgacg accagtgtgg ccgacgaagc
     4981 catgcgcgtc caggccggca tgcggcgaga agcctcggcc gggccaccaa agaggaagcc
     5041 gccgaagagc aggacggccc acaggatgag caggccgatg agccagtagc gttgggcggg
     5101 gttggatagt gattgaaaca taagactccc ttaggtagga cgacggacga gcggactcct
     5161 gcccgccgac gacgtgaccc ctgtcgcgtg acgacagacc acagaccacg gattcctgtc
     5221 aaatttagcg caatgacgag aagaagccaa aaacgaagat gagtatgctc cctctcatcg
     5281 cagcctgtgc ctcttggccg gtcggcgtca gtggtcagcg ggcaggagtc cgctcgtcgg
     5341 ctggcggtca tatccgccct aaactggtaa aatcacgctc atggccgaac gcgtggagat
     5401 cagcggctct gtggagcgcg tgacttatta caacgaggag aacggctaca ccgtcctgcg
     5461 gctgcggccg gacacgcgcg
//
LOCUS       DAOMQG010000230         5449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6330_length_5449_cov_11.087505, whole genome shotgun sequence.
ACCESSION   DAOMQG010000230 DAOMQG010000000
VERSION     DAOMQG010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_5449_cov_11.087505"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..576
                     /gene="rplI"
                     /locus_tag="PLC06_11760"
     CDS             52..576
                     /gene="rplI"
                     /locus_tag="PLC06_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRO91165.1"
                     /translation="MKVLLKQDVDNLGYAGEVYTVADGYGRNFLLPKGLAVKATPDVM
                     KQAESWRKKADTHRAELRAEYDVLSEKIRAVSMSFTARAGDSGKLFGSITTHQIADQL
                     NEILGTEIDRRKVGTEALRQIGEHMVPVRLSADHHPEFRVVILQEGAPVEAAAAAAAQ
                     VAEVVDEAVAEVEA"
     gene            646..1593
                     /locus_tag="PLC06_11765"
     CDS             646..1593
                     /locus_tag="PLC06_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRO91166.1"
                     /translation="MDEIGHILREARENRGLTLEEVQAKIRINARYLAAFETGQYDAL
                     PTPVHARGFLRNYARFLGLDPQPLLDRYLTVQGMEAQVMVARPNQEITPDNPLPERQD
                     QPFFDPVNMEVNGKGFDAVSNGGGSSSMLQIVIIIALVIALALIANRFIPILFGRGGE
                     ANVVQEFTSAVQDAVAEVTGATATPTVTPAATQESASGSAIFVDGTEQPIMDTSRNNP
                     GGVATPTATRPPLPATLDEIALKLDILERTWVEVTIDDSVVFSGIAKTNDTFEWTAQN
                     EAKIVTGNAIGVFVTINDVALGRLGGRGERHEETWRTTQ"
     gene            1604..2995
                     /gene="rimO"
                     /locus_tag="PLC06_11770"
     CDS             1604..2995
                     /gene="rimO"
                     /locus_tag="PLC06_11770"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRO91167.1"
                     /translation="MSKAKQKKQFYLLSLGCSKNTVDSTSMADLLYRAGFSATDDPDD
                     AGVLIVNTCGFIESARQESIGALRELADLKGQNQLLIAAGCMAQRYGDEVVEWVPGID
                     GVIGTRRWMDIVPFVRELRRRKHPEPLYHLPDEAATVGRDERNVLRATVQGASAYLKI
                     ADGCRRPCAFCAIPQIKGTHVSRPPAAIVAEAVRLQAAGVRELILIAQDTTDYGHDLG
                     IKNGLTYLLKEITAAAPDIPWIRIMYAYPGYVTDELIETMATTPQILPYLDIPLQHGH
                     RETLKRMRRPANIEWVYGTLEKLRAAMPDIAIRTTFIVGYPGETDEEFDALKTFVHDL
                     QFDRVGAFTYSYEASTPSATVSWQVEEAVKQARQAELMELQQAISLSRNQAQVGRTLP
                     VLIEGHGDGLSVGRTYRDAPEIDGLVLIPGELPLGELVPVRIDGAMTYDLTGQAVISG
                     SVDQWISTIALTD"
     gene            3051..3596
                     /locus_tag="PLC06_11775"
     CDS             3051..3596
                     /locus_tag="PLC06_11775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRO91168.1"
                     /translation="MRARLEWLTLIVLVALIGAGWIYLSREAGGNNEPMPLTTAPYIG
                     NLAPDFTLQTFDDRTVTLRDFTADGGMPVVLNFWATWCPPCRVEMPYFENANALYDGR
                     VAILGLNQAESAALIGEYARVHGLTYPLLVDEDMTVNHLYGVLNLPTTIFIDKNGVVR
                     EVLIGTISQGVLEDRIEKLLE"
     gene            3628..4161
                     /locus_tag="PLC06_11780"
     CDS             3628..4161
                     /locus_tag="PLC06_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRO91169.1"
                     /translation="MRRIRYQVACSLDGYIADSAGKTGWIVDEPTIDFGALFDEFDTL
                     LMGRLTYEGIVKEADGFWGKRVLVFSKTLRQEDHPQVTVLSNDIEAVLNQLRSEPGKD
                     IWLFGGGELFQSLLALDCVDTVEPAIVPVLLGGGRPLLPTPSMLHRLDLTSHRVFPSG
                     IVWLEYTVRPSDESDVR"
     gene            4205..4957
                     /locus_tag="PLC06_11785"
     CDS             4205..4957
                     /locus_tag="PLC06_11785"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRO91170.1"
                     /translation="MLPYLNIGPIGLPTGPLTYLFGIWLALFAVDRAARRLSRDPESH
                     YALASVALLGGFIGARLVFVLLHWSSYDDNLLGIVWPLTSGYNVFGGVFIGLAAAFFY
                     GRWRDLALWPTLDVLAPGVLVFLMTVSLAEFLGGAGYGSLTSLPWGIDQFGVRRHAVQ
                     LYEVLAGVVALTAWWATTSLRFRGMEGRPFLVAAAVYAGGRLFVDAYKETTPLIMNGI
                     HVVQIVALGVTLMALVLLARLRPQSEVVQAGE"
     gene            complement(4942..5241)
                     /locus_tag="PLC06_11790"
     CDS             complement(4942..5241)
                     /locus_tag="PLC06_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRO91171.1"
                     /translation="MRRELSPVHPGEVLLEEFLIPMDLSQNRLALDIHVPARRINEIV
                     QGKRRITTDTALRLARYFDMSPQFWLGLQLDYDLDVAEDELSERIEREVRVYSPA"
     gene            complement(5250..>5449)
                     /locus_tag="PLC06_11795"
     CDS             complement(5250..>5449)
                     /locus_tag="PLC06_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRO91172.1"
                     /translation="ALRKLRMLNRAQSLQDLRVPPANHLEKLSGNRSGQYSIRINDQW
                     RICFAWRDEDAYEVEIVDYHS"
BASE COUNT         1069 a         1625 c         1650 g         1105 t
ORIGIN      
        1 ctgtagagtt gcccggacct gcgatactcg aaaaatcagg agtctgtgtc gatgaaagtg
       61 ttattgaagc aagacgttga caatctgggc tatgccggag aagtctacac cgtggctgac
      121 ggctacggcc gcaatttcct gctgcccaag ggattagctg ttaaagcaac ccccgacgtg
      181 atgaagcagg ccgaatcctg gcgcaagaag gctgacaccc accgcgccga attgcgggcc
      241 gaatatgatg tgttgtcgga gaagattcgc gccgtctcga tgtccttcac tgcccgcgcc
      301 ggcgatagcg gcaagctgtt cggctcgatc acgacccatc aaatagccga ccagctaaac
      361 gagatactcg gcacggagat cgatcgccgc aaggtcggca ccgaagcgct gcgccagatc
      421 ggcgagcaca tggtgccggt tcgcctgagc gccgatcacc acccggagtt ccgtgtggtc
      481 atcctgcaag agggcgctcc ggttgaagcc gccgcggccg ctgcggccca ggttgccgag
      541 gtggtggacg aagcggtcgc cgaggttgag gcctaactcg cggcaattgc cgctcaccgg
      601 tagcggtcac caagtgactg cccggaagga acgatcgacg cccgcatgga cgagatcggt
      661 cacatattgc gcgaagcccg cgaaaacaga gggctgacgc tggaagaagt acaggcgaaa
      721 atccgtatca acgcgcgcta tctggcggcg tttgagaccg gtcaatatga cgcgctaccc
      781 acgcccgttc acgcgcgtgg gtttctgcgt aattacgccc gcttcctggg gcttgacccg
      841 caacctttgc tggatcgcta cctgactgtt caggggatgg aagcgcaggt catggttgcg
      901 cgccccaatc aggagatcac gcccgacaat ccgttacccg aacgccagga tcagccgttc
      961 tttgatccgg tgaacatgga ggtcaacggc aaggggttcg acgcggtcag caatggcggc
     1021 ggatcgagca gcatgctcca gatcgtgatc atcattgccc tggtcatcgc gctggccctg
     1081 atcgccaacc gcttcatccc gattctcttc ggccgtggcg gcgaggccaa cgtcgtgcag
     1141 gagttcacct cggcggtaca ggatgccgtg gccgaggtca cgggtgctac cgcaacgcct
     1201 accgtcaccc ccgcggcgac tcaggagtca gcctcgggat cggcgatttt cgtcgatggg
     1261 acggaacagc cgatcatgga caccagccgg aataaccccg gtggcgtggc cacccccacg
     1321 gctacacggc cgcccttgcc cgccacgctt gacgagatcg ccctcaagct cgatattctg
     1381 gagcggacgt gggtcgaggt gacgatcgat gacagcgtgg tcttcagtgg gatcgccaag
     1441 accaatgata cgttcgaatg gacggcccag aacgaagcca agatcgtcac cggcaacgcc
     1501 atcggtgtgt tcgtgacgat caacgacgtg gctctcggcc gtctgggcgg ccgcggcgag
     1561 cggcatgaag agacctggcg gacaacgcag tagcaggcaa ttgatgagca aagccaagca
     1621 gaaaaagcag ttctatctcc tctctctggg gtgtagcaag aacacggttg attccaccag
     1681 catggccgac ctgctctata gggccggctt ttcggcgacc gacgaccccg acgacgccgg
     1741 cgtgctcatc gtcaacacct gcggcttcat cgagagcgcc cgtcaggaat cgatcggcgc
     1801 gctgcgcgag ttggccgacc tgaagggaca aaaccagttg ctcatcgccg ccgggtgcat
     1861 ggcccagcga tatggtgatg aagtggtcga gtgggtgccg ggcatcgatg gcgtcatcgg
     1921 cacacgccgc tggatggaca tcgtgccttt tgtgcgcgaa ttgcgtcgcc gcaagcaccc
     1981 ggaaccgctc tatcatctgc ccgacgaggc ggccaccgtc ggccgcgacg agcgcaacgt
     2041 attgcgggcc acggtgcagg gggccagcgc ctacctgaag atcgccgacg gctgccgacg
     2101 accgtgcgct ttctgcgcca ttccgcaaat caagggcacc cacgtcagcc gcccgcccgc
     2161 ggccatcgtc gctgaagccg tccgtctcca ggcggccggg gtgcgcgaac tgattcttat
     2221 cgcccaggac accactgact acggtcatga tctgggcatc aaaaacggcc tcacctatct
     2281 cctcaaggag atcacagccg ccgcacccga catcccctgg attcgcatca tgtatgccta
     2341 tcccggctac gtcaccgacg aactcatcga gacgatggca accacgccgc aaatattgcc
     2401 ctatctcgac atccctttgc agcatggcca ccgcgagacg ctgaagcgca tgcgccgccc
     2461 ggccaacatc gaatgggtct acggcacgct ggagaagctg cgcgcggcca tgcccgacat
     2521 tgctatccgc acgaccttca tcgtcggcta ccccggcgag acggacgaag agttcgacgc
     2581 gctgaagaca tttgtgcacg atctccagtt cgaccgcgtc ggcgcattca cctattctta
     2641 tgaggcgtcc acaccatcgg ccactgtctc gtggcaggtg gaagaggccg tcaaacaggc
     2701 acgacaggcg gaactaatgg agctgcagca agccatctcg ctgtcgcgca atcaggccca
     2761 ggtcggccgc actttgcctg tcctcatcga ggggcatggc gacggcttga gcgtcggccg
     2821 cacctatcgt gatgcgccgg agatcgacgg gctggtgctc atccccggcg aactaccgct
     2881 gggcgaactg gtgccggtgc gcatcgacgg ggccatgacc tatgatctga ccgggcaggc
     2941 agtgatcagt ggatcggtag atcagtggat cagtacaatt gcactgactg actaacctgc
     3001 tgcgttactg acctactaat ttactgactt gctgacccat tgatcaaacg atgcgcgcac
     3061 ggctggagtg gctcactctc atcgtcctgg tcgccctgat cggcgcggga tggatttatc
     3121 tctcgcgcga ggcgggcggt aataacgagc cgatgccgct cacgaccgcg ccctacattg
     3181 gcaacctcgc ccctgatttc acgctccaga ccttcgacga ccgaaccgtc acattgcgcg
     3241 acttcacggc cgatggcggg atgccggtcg tcctgaactt ctgggccacg tggtgcccgc
     3301 cctgccgcgt ggagatgccc tatttcgaga acgcaaacgc cctttatgac gggagggtag
     3361 cgatcctcgg gctaaatcag gctgagtcgg cggcgcttat cggcgagtat gcccgcgtcc
     3421 acggcctgac ctaccccttg ctggtagacg aagatatgac cgtcaatcat ctttatgggg
     3481 tactcaacct gccgacgacg atttttatcg acaagaacgg cgtcgtccgc gaggtgctga
     3541 tcggcacgat cagtcagggg gtattggagg atcgaatcga gaagctgctg gaataaggcc
     3601 tgggcaatag cccaaatgga gatatcgatg cgtagaatcc gctatcaggt cgcatgcagt
     3661 ctggatggat acatcgccga ttcggccggc aagacgggct ggattgtcga cgaacccacc
     3721 atagactttg gcgcgttgtt cgacgagttc gacacccttc tcatgggacg ccttacctac
     3781 gaagggatag tgaaagaagc cgacggattc tggggaaagc gggttctggt tttctcgaag
     3841 acgcttcggc aggaagacca ccctcaagtt accgtccttt ccaatgatat tgaggctgtt
     3901 ctcaatcaat tgcgctcgga accggggaag gacatttggc tctttggggg cggcgaactc
     3961 tttcaaagcc tgctcgccct ggactgtgtc gataccgtgg agccggccat tgttcctgtc
     4021 ctgctgggtg ggggacgtcc cttgctaccg acgccgtcga tgctccatcg gctggatttg
     4081 acttcccacc gggtctttcc aagcggcatt gtatggcttg aatatacagt gcggccatcc
     4141 gatgaatccg atgtccggtg atgttttgga agtgtaacgt aattaccttg ttcgcaattt
     4201 actgatgctc ccttacctca atatcggccc catcggcctg ccgaccgggc cgctgaccta
     4261 tctcttcggc atatggctgg ccctctttgc cgtcgatcgg gcggcgcggc ggctcagccg
     4321 cgatcctgaa agccattatg cgctcgcgtc ggttgccctg ctgggtggtt tcattggcgc
     4381 gcgactggtc ttcgtcctgc tgcattggtc atcttacgac gacaatctgc tcggtatcgt
     4441 ttggccgctg accagcggct acaacgtctt cgggggcgtg ttcatcgggt tagcggcggc
     4501 cttcttctac ggccgctggc gagacctcgc tctttggccc acgctggatg tcctcgcgcc
     4561 gggggtcctc gtgttcctca tgacggttag cctggccgag tttctcggcg gcgcggggta
     4621 tggttcgctg acgtcgttgc cgtgggggat tgatcagttc ggcgtgcgac gacatgccgt
     4681 ccagctctat gaggtgctgg ccggcgttgt tgcgctgacg gcgtggtggg cgacgacatc
     4741 gttgcgattc cgggggatgg aggggcggcc gttcctggtg gctgcggccg tttatgccgg
     4801 ggggcggttg tttgtggatg cctataaaga gacgacgccg ctgatcatga acggcatcca
     4861 tgttgttcag attgtggctc tgggggtgac actgatggcg cttgtcttgc tggcgcgttt
     4921 gaggccgcaa agcgaagtgg ttcaggccgg cgaataaact cgcacctcgc gctcgatgcg
     4981 ctcacttaac tcatcctcgg ccacgtccag gtcgtagtcc agctgcaagc cgagccagaa
     5041 ctgcggcgac atatcaaaat agcgcgccaa tcgcaaggct gtgtccgttg taatgcgccg
     5101 cttgccctga acgatctcgt tgatccgtcg cgccggtacg tggatgtcaa gagccaggcg
     5161 attttgactc agatccatcg ggatgaggaa ttcctctaac aatacctcgc cggggtgtac
     5221 gggggacaac tctctacgca tggatatcct catgaatgat aatcaacgat ctcgacctcg
     5281 taggcatctt catcccgcca ggcgaagcaa atgcgccact gatcgttgat ccgtatgcta
     5341 tattgaccgg aacgattgcc ggacaacttc tcaaggtgat tcgcaggagg aacacgtaaa
     5401 tcctgaagag attgtgctcg attgagcata cggagttttc gcaacgcta
//
LOCUS       DAOMQG010000231         5392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6412_length_5392_cov_7.701330, whole genome shotgun sequence.
ACCESSION   DAOMQG010000231 DAOMQG010000000
VERSION     DAOMQG010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6412_length_5392_cov_7.701330"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLC06_11800"
     CDS             complement(<1..779)
                     /locus_tag="PLC06_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRO91173.1"
                     /translation="MTNPTHDRFETIAVHAGVHPDPATGAIMTPIYQTSTFVQTDVNE
                     HKGYDYSRAGNPTRSALEEALAGLEGGRYGLSFSSGLAAIDTLLRLVKPGERVICGND
                     LYGGTFRLFDKVLADYGIIFGYVDTSDTDAVRAALAGGPARLVWLETPTNPTLRLTDI
                     AAVSEAARAAGVWVAVDNTFASPALQRPLALGADFVVHSTTKYLGGHSDVVGGAIIMN
                     DPDAHERLKFLQKAVGAVSGPMDCFLTLRGIKTLGIRMRTH"
     gene            908..1339
                     /locus_tag="PLC06_11805"
     CDS             908..1339
                     /locus_tag="PLC06_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRO91174.1"
                     /translation="MKIRPDKTTCIILFILALLVAGCRATADLGGTSWQLTAYGPADA
                     PLDAEAPASIQFEDNGRLGGFTGCNTFFGHYRVDDGQISLRDGELTFTVVDCDASTPE
                     GRQDEFFRAWLTDIGDYSQTDDGLQLVFDEGRQIAVYVPAD"
     gene            complement(1397..2359)
                     /locus_tag="PLC06_11810"
     CDS             complement(1397..2359)
                     /locus_tag="PLC06_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO91175.1"
                     /translation="MEPIRIELPLGWSMGSVNAYLFTQPEVVLVDAGVKSEACREALV
                     DALAAHGLAVRDIERVVITHPHVDHFGLTGRIVAESDATVWICELGAPWLTDPDSMWA
                     GRMAYYQRDLLPALGLPSKTNELILGGLRELATQADAVPVGRTVTFRPDGVLQLGGAT
                     WQVVHAPGHACAQTIFYQPETRQLLSADMLLAVTPAPVIDHPSPGKRREPALPHFMHS
                     LDMVNALDIGMVFPGHGRPFDDHRAVIARQRERIAGRKAQCLELIREGQSTIPELLEI
                     MYAHQPPGSRVAGLWMLIGYLDLLLDEGEIVEDIIADMNHYHIP"
     gene            complement(2462..5242)
                     /gene="ppc"
                     /locus_tag="PLC06_11815"
     CDS             complement(2462..5242)
                     /gene="ppc"
                     /locus_tag="PLC06_11815"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HRO91176.1"
                     /translation="MTREKQERLSEDIHLLGDILGHVIRRQAGIEIFELEERIRALAK
                     TRRLESDPAIDTRLAYVVGRLDSAEAELVARSFTTYFELVNLAEEQHRVRVLRERERD
                     VHPQPLSESVAGAVAALWKAGVDEFEMRRLLNRLDIELVFTAHPTQAKRRTILTKLRN
                     IAAALTELDVRDLLPSERQALVDQVTAEVTLMWVTDRSRTSRPTVTDEVRTGLFYFDT
                     TLWDVLPAVYRDMAAALAQHYPALHPPDRFLTYASWIGGDRDGNPNVTAGVTAETLRL
                     HRGLALEKHRAAARRLDRALSVSNQLADVPEAITDAVDAYGEPEGHLAFLHARYPHET
                     YRHLSAQLGADLAEASAGDMIARLRGEEMPPLRMRTMDNLLEPLTLMDRSLRATHMAE
                     IADADLEQSLLQARVFGLHAARLDLRQYSDYNTAVLDELFGRLGWFSGFGEMDGPARA
                     AILTDALARPIPDLSGFDGLSAEAAETLELFRVVKRAVDYYGPELFGPYVVSMTHGPE
                     DILAPLLLARWHGLCLRDDGRPEGMTFAPLFETRDDLRAAPEVMTALFAHPGYAPHLA
                     RAGRRQTIMIGYSDSNKDAGYLAANWELYQGQDALARTCADHDVVMALFHGRGGTIAR
                     GGGPANRSILAQPPGSVGGRIRITEQGEVISDRYSHPAIANRHLQQVVHAVLMASAPE
                     QYRLLPRPKPDWSTAMDELAATSYRAYRALIYETPELLTYWSQATPMDEISQMRIGSR
                     PSRRSGAVTFDSLRAIPWGFSWMQCRHVLPGWYGVGEALAGFGRAPERVALLQEMYRD
                     WSFFRNLLDNSQVSMAQADMGIAGLYAGLVEDARVRDLIFGQVEAGFNRTREAILLTT
                     GGRELLDNDPVLQRSVRRRNPYIDPLNFIQVSLLRRLRGLPNPESTEAAHIRDTIFLT
                     ISGIASGLKNTG"
BASE COUNT          996 a         1668 c         1789 g          939 t
ORIGIN      
        1 cagtgggtgc gcatacggat gcccagcgtc ttgatgccgc ggagcgtgag gaagcagtcc
       61 atcggccccg ataccgcgcc gaccgccttc tgcaggaact tgagccgctc atgggcgtcg
      121 gggtcgttca tgatgatcgc cccgccgacg acgtcagaat ggccgcccag gtacttggtc
      181 gtggaatgga cgacgaaatc ggccccaaga gccagcggcc gttgcagcgc gggcgaggcg
      241 aaggtattgt ctaccgccac ccagaccccg gcggcgcgcg ctgcctcgct gacggcggcg
      301 atgtcggtca ggcgcagcgt cgggttggtc ggcgtctcca gccacacgag gcgggccggc
      361 ccgccggcca gcgcggcgcg aacggcgtcg gtgtcggacg tatccacgta gccgaagatg
      421 atgccataat cggccagcac cttgtcgaac agacggaatg tgccgccata gaggtcgttg
      481 ccgcagataa cccgctcgcc gggcttgacg aggcgcagca gcgtgtcgat ggcggccagg
      541 cccgacgaga aagagaggcc atagcggccg ccctccagcc ccgccagcgc ctcctccagc
      601 gccgagcgcg tcgggttccc ggcgcgggag tagtcgtagc ccttgtgctc gttgacgtcg
      661 gtctggacga aggtcgaagt ctggtagatg ggggtcatga tcgcgccggt ggccgggtcg
      721 ggatggactc cggcgtggac ggcgatagtt tcgaaacggt cgtgggtggg attggtcata
      781 gaggatctcc aattagcgaa tggtggtagt tttcggcgcg gatgtcattg tatcatatcc
      841 ataagctgta gagcgatttg gcaaatcgct tgcgatttgg caaatcgctt ctaccggaga
      901 actgccgatg aaaattcgtc ccgacaaaac aacttgcatc atcctcttta ttttggcgct
      961 cctcgtagcc ggttgccggg ccacggccga cctgggcggg acttcatggc aattaacggc
     1021 ctatggcccg gccgacgcgc cgctcgatgc cgaggccccg gcgtctatac agtttgagga
     1081 taacggccga ttgggtggat ttaccggctg caacaccttc ttcgggcatt accgggtgga
     1141 tgacgggcaa atctcgctgc gcgacggcga gttgacgttc actgtagtag actgtgatgc
     1201 ttccacgccg gaagggaggc aagatgaatt cttccgtgcc tggttgaccg atatcggcga
     1261 ctattcacaa acggacgatg gccttcagct tgtttttgat gaggggcgac agatcgccgt
     1321 ttatgtgccg gcggattagc ccacgtgtag agcaggcatt cctgtctgcg aagaggcgca
     1381 gacaggaatg tctgctctac ggtatatggt aatggttcat gtcggcaatg atatcttcaa
     1441 caatctcccc ctcgtccagc agcaaatcca gatagccgat cagcatccac aaccccgcca
     1501 cccgactccc cggcggttgg tgagcgtaca tgatctccag cagttcgggg atagtcgatt
     1561 gcccctcgcg gatgagttcc aggcattgcg ccttgcgccc ggcgatacgc tcccgctggc
     1621 gggcgatgac cgcccggtgg tcgtcaaacg gccgcccgtg gccgggaaag accatgccaa
     1681 tatccagcgc gttcaccata tcgagcgagt gcatgaaatg agggagtgcc ggttcccgcc
     1741 gcttgcccgg cgagggatga tcgataaccg gcgcgggcgt gacggccagc aacatgtcgg
     1801 ccgaaagcag ttggcgggtt tcgggctggt agaagatagt ctgggcacaa gcatggccgg
     1861 gggcatggac gacttgccac gtcgcgccac ccagttgcag cacgccatcc ggccgaaagg
     1921 tgacggtgcg cccgaccggc acggcatcgg cctgggtcgc cagctcgcgc aatccgccaa
     1981 gaatcagctc attcgtcttc gacggcaaac ccagcgcggg tagaagatcg cgctgataat
     2041 aagccatacg cccggcccac atgctgtcgg ggtccgtcaa ccatggcgcg ccaagctcgc
     2101 aaatccagac tgtcgcgtcg ctctcggcga cgatgcgccc ggtcaggccg aaatggtcga
     2161 cgtggggatg agtgatgacg acacgctcga tgtcgcgcac ggccaggcca tgagccgcca
     2221 acgcatcgac cagcgcttcc cggcacgcct ccgacttgac ccccgcatca accagcacaa
     2281 cctccggctg cgtgaacaga taagcgttga ccgaccccat cgaccagccg aggggtaatt
     2341 caatacgaat gggttccata tggatattat ccgcgggatc gacggaatga gtagagaagg
     2401 agcgacagac cacggaaggc ctcttcccct tttctccccc tctccctttc tctctccccg
     2461 cctaccccgt attcttcaat cccgacgcga taccgctgat cgtcaggaag atagtgtcgc
     2521 ggatatgtgc ggcttcggtg ctttcgggat tgggcaggcc gcgcaggcga cgcagcaagc
     2581 tgacctggat gaagttgagc gggtcgatat aaggattgcg gcggcgaacg gagcgttgca
     2641 gcaccgggtc gttgtcgagt aactcgcggc cgccggtggt cagcaggatc gcctcgcgcg
     2701 tccggttgaa ccccgcctcg acctgaccaa atataaggtc gcgcacccgc gcatcctcga
     2761 caagaccggc atagagaccg gcgatcccca tatcggcctg ggccatcgac acctgggaat
     2821 tgtcgagcag attacggaag aacgaccagt cgcggtacat ctcctgcaac agcgccactc
     2881 gttccggcgc gcggccgaac ccggccagcg cctcgccgac gccataccag ccgggcagta
     2941 cgtgacgaca ttgcatccag ctaaagcccc acggaatagc ccgcaaactg tcgaacgtga
     3001 ccgcccccga ccggcgcgac ggccgcgagc caatgcgcat ctggctgatc tcatccatcg
     3061 gcgtggcctg gctccagtag gtgagtagtt ccggcgtctc gtagatgagg gcgcgatagg
     3121 cgcgatagga cgtggccgcc agttcatcca tggctgtcga ccaatccggc ttcggtcgcg
     3181 gcagcaggcg atattgctcc ggcgcgctgg ccataagcac ggcgtggacg acctgttgca
     3241 gatggcggtt ggcgatggcc gggtggctat agcgatcgga gataacctcg ccctgctcgg
     3301 tgatgcggat gcggccgccg acggagccgg gcggctgggc caggatggag cgattggccg
     3361 ggccaccgcc gcgggcgatc gtgccgccac ggccatgaaa cagcgccatg acgacgtcgt
     3421 ggtcggcgca cgtccgggcc agcgcgtcct gcccctgata aagctcccag ttggcggcca
     3481 gatagcccgc gtccttgttg ctgtcggagt agccgatcat gatcgtctga cggcggccgg
     3541 cgcgggccag atggggagca tagccgggat gggcgaagag cgcggtcatc acctccggcg
     3601 cggcccgcag gtcgtcgcgt gtctcgaaca acggcgcgaa ggtcatccct tccggccggc
     3661 catcgtcacg cagacaaagg ccatgccagc gagccagcag caggggagcc aggatatcct
     3721 ccggcccgtg ggtcatgctg acgacgtagg ggccgaacag ctcagggcca tagtaatcga
     3781 cggcgcgctt gacgacccgg aacagttcca gcgtctcggc cgcttcggcc gacaggccgt
     3841 cgaagccgct caggtcgggg atggggcggg ccaacgcatc ggtgagaatg gcggcgcggg
     3901 ccgggccatc catctcgccg aacccgctga accagcccag gcggccgaaa agctcgtcga
     3961 ggacggccgt gttgtagtcg ctgtattgtc gcaggtcgag ccgcgcggcg tggagaccga
     4021 aaacgcgcgc ctgcagcagg ctttgctcca ggtcggcatc ggcgatctcg gccatgtggg
     4081 tggcgcgcaa cgagcgatcc atcagcgtca gaggctccag cagattatcc attgtgcgca
     4141 tgcgcagcgg cggcatttcc tcccctcgca ggcgggcgat catatccccg gctgatgcct
     4201 ccgccagatc cgcgcccagc tgggccgaca ggtggcgata ggtctcatga ggatagcggg
     4261 cgtggagaaa ggccaggtgg ccttccggct caccataagc atcgacggcg tcggtgatcg
     4321 cttcgggcac atcggccagc tggttggaga cgctcagggc gcggtcgagg cggcgggcgg
     4381 cggcgcgatg cttctccagc gccagaccgc gatggagccg cagcgtctcg gccgtcacgc
     4441 cggcagtgac gttggggttg ccgtcgcggt cgccgccaat ccacgaagcg taggtcagga
     4501 agcgatcggg cgggtgcaga gccgggtaat gctgggccag cgccgcggcc atgtcacgat
     4561 agacggcggg cagcacgtcc cacaaggtcg tatcaaagta gaacaggccg gtgcgcactt
     4621 cgtcggtgac ggtcggccgc gacgtgcggc tgcggtcggt gacccacatc agcgttacct
     4681 cggccgtaac ctgatcgacc agcgcctgcc gctccgaggg cagtaaatcg cgcacgtcca
     4741 gctcggtcag cgcggcggcg atgttgcgca gcttggtcag gatggtgcgg cgcttggcct
     4801 gggtggggtg ggcggtgaag accagttcga tgtccagccg gttgagcagg cggcgcatct
     4861 caaactcatc aacgcccgcc ttccagagcg cggccacggc cccggcgacc gattccgata
     4921 gcggttgcgg atgtacgtcg cgctcgcgct cgcgcagaac gcggacgcgg tgctgctcct
     4981 cggccagatt gaccagctcg aagtaggtgg tgaagctgcg ggcaaccagc tcggcctcgg
     5041 ccgagtcgag acggccaacg acataggcca gccgcgtgtc gatggccggg tcgctctcca
     5101 gacgacgggt cttggccagg gcgcggatgc gctcctccag ctcgaatatc tcgatcccgg
     5161 cctgtcgccg gataacgtgg ccgaggatat cgcccagcag gtggatgtct tcgctcagtc
     5221 gttcctgttt ttcgcgggtc atgggcatag ttgggcctca gggatgaatc cgcagatttc
     5281 gcggatttcg cggattagaa accgagtttt tcggaaaaac ccggtttctg gaggttgctg
     5341 gatagcagct acaaagtaat cacctgatcc ggcatattcc ctgccagcgc cg
//
LOCUS       DAOMQG010000232         5374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6445_length_5374_cov_9.647490, whole genome shotgun sequence.
ACCESSION   DAOMQG010000232 DAOMQG010000000
VERSION     DAOMQG010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_5374_cov_9.647490"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLC06_11820"
     CDS             complement(<1..251)
                     /locus_tag="PLC06_11820"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRO91177.1"
                     /translation="MPEPDYHVLADLAEDLLPYITESLGAVIAEKGVVSFVLSGGGTP
                     RDLYRLLGRTSDADGVPWDKVHFFWGDERLVPPDDPGSN"
     gene            complement(244..1242)
                     /gene="glk"
                     /locus_tag="PLC06_11825"
     CDS             complement(244..1242)
                     /gene="glk"
                     /locus_tag="PLC06_11825"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HRO91178.1"
                     /translation="MLLAGDIGGTKTVLALFDVDEANGIARHPLMERTFPSQQYQSLE
                     LIIEEFLRECDYSVSAGSFGVAGPVMGNRAQVTNLPWMIDAEAMRERFGFTVHLLNDL
                     EALATAVPFLGATDLITLSEGAPVERGAIGVIAPGTGLGEGFLVWNRPAGKYESYPSE
                     GGHCAFGPTTLLQLEMLNYWLPRMGHVSYERLCSGIGIPNIYTFLRETARYPEPDWLR
                     DQLDEAADPTPVIVKAAVAGEAEICAATLELFMEILGDQAGNLALTVLATGGIFLGGG
                     IPGRILPQLQKGPFMKFFQDKGRFSEMMSRIPVHIIYNPKAALYGTAYDALALTHA"
     gene            complement(1406..1738)
                     /locus_tag="PLC06_11830"
     CDS             complement(1406..1738)
                     /locus_tag="PLC06_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91179.1"
                     /translation="MTSLIEKLRELRSRTGEGAGASAGASLDEQSVERLMRLLCETRD
                     DELSCEEVFGCLDEYVECLEAHHEFGHRKQLVEHHLKLCTDCRDELDALIHALEDADA
                     AGHVDIAS"
     gene            complement(1786..2433)
                     /locus_tag="PLC06_11835"
     CDS             complement(1786..2433)
                     /locus_tag="PLC06_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO91180.1"
                     /translation="MSATNARTNEQWVADLGAEGPAREAALADLRVVLANGLRRGLIG
                     QVDTSAPEFDVLIDDFIQDALLKILENLDTFAGRSLFTTWANKVALNIGLTELRRKRW
                     RDTSLDGLMQTEEGEFTPSFVADPAPRPEELTERKELMGYVNHLINEELTEKQRTALT
                     AAVIQGRPLSEVAWMMDSNQNAVYKLVFDARQRLRRRLAEDNLTPEDILASFVGR"
     gene            2820..3947
                     /locus_tag="PLC06_11840"
     CDS             2820..3947
                     /locus_tag="PLC06_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91181.1"
                     /translation="MDIIKQKPGLAALVAFIAGLIIGLVVLGWGLFPVQYTGADPSGL
                     FPEYQAVFVRNAAELYAFDNNQEKLRGALSGWGGDAVACELAKSSIDPADQQRMIAAA
                     TIVNGTGCGSLTSPGTQATVPVAPPGATAALPGAESSGGSGSLPTMLLLLLLALLLLA
                     IFYVWNRRRTLMQGTDDEDADYTENGVSRKSARPSYFRPAAAASATATAAESRAVPIA
                     RFRTAYTHGHDTYDDSFSIENANGDFLGECGVGISETLGASASRNVTAFEVWLFDKND
                     IRTITKVIMSDHAYFDEALKAKLAPKGEPVLARENETIVLETATLIINAEITEMEYGE
                     SGDMPDASYFERFTIELSAWAKEGDYAASQPAGDEEDLLNY"
     gene            complement(4105..5259)
                     /locus_tag="PLC06_11845"
     CDS             complement(4105..5259)
                     /locus_tag="PLC06_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRO91182.1"
                     /translation="MIRKRPQLEEIESMSLAPYGLRSAESRGRAYPEPESATRPAFTR
                     DRDRIIHTTPFRRLMYKTQVFVFFEGDHYRTRLTHTLEVAQLGRSLARGLGGNVDLTE
                     AICLAHDLGHPPFGHAGEHMLNILMADHGGFNHNTQSYRIVTKLEDSYPDFPGLNLTY
                     ETREGMLKHETDYDRSEAAEYEPNKRASLEAQIANFADEIAYNAHDLDDGLRAGLFTL
                     TDLDDLAIWRELREVAGWKPGEHFTSMHRHEIIRELIGRSIGDVMTRTAEALDANDID
                     SPEKLQRHPENVVGYTPDFGQQVRALKKFLFDRMYRHYRVMRMQTKAERFVAELFDAY
                     VKEPHMLPDETQARLETAPMERVVADYIAGMTDRYALDDWQRLYDPYIRA"
BASE COUNT         1051 a         1620 c         1638 g         1065 t
ORIGIN      
        1 tagttgctgc cggggtcatc gggcgggacg aggcgctcgt cgccccaaaa gaagtgaacc
       61 ttgtcccacg gcacgccgtc ggcatcggat gtccggccca gcagccggta caggtcgcgc
      121 ggcgtgccac ccccggacag gacaaagctc accacgcctt tttcagcgat gaccgcccca
      181 agggactccg taatgtacgg caggagatcc tcggccaggt cggcgaggac gtggtaatca
      241 ggctcaggca tgggtcagcg ccagcgcgtc ataggccgtg ccatagagcg cggctttggg
      301 gttgtagatg atgtggacag ggatgcggct catcatctcg ctgaatcgcc ccttgtcctg
      361 gaagaacttc atgaacggcc ccttctgcag ttggggcagg atgcggccgg ggatgccgcc
      421 gccgaggaaa atgcccccgg tcgccagcac ggtcagcgcc agattgccgg cctgatcgcc
      481 caatatctcc atgaacagct ccagcgtggc cgcgcagatc tcggcttctc cggccacggc
      541 cgccttgacg atgaccggcg tcgggtcggc ggcctcatcc agctggtcgc gcagccagtc
      601 gggttcgggg tagcgggccg tctcgcgcag gaaggtataa atgttgggga tgccgattcc
      661 ggagcaaagg cgctcgtagc tgacatgccc catgcgcggc agccagtagt tgagcatctc
      721 cagctgcaga agcgtcgtcg ggccaaacgc gcaatggcca ccttccgacg ggtaggactc
      781 atatttgccg gccggtcgat tccagacgag gaaaccctcg cccagccccg tgccgggggc
      841 gataacgccg atggcgccac gctccaccgg tgcgccttcg ctgagcgtga tgaggtcggt
      901 cgctcccaga aacggcacgg ccgttgccag cgcttccagg tcattcaaca gatgcacagt
      961 gaagccgaaa cgctcgcgca tggcctcggc atcgatcatc catggcaggt tggtcacctg
     1021 ggcgcgattg cccatgaccg gcccggcaac cccaaagctg ccggcggaga cgctatagtc
     1081 acattcgcgc agaaactcct cgatgatgag ttccagcgat tggtactgct ggctggggaa
     1141 ggtgcgctcc atgagggggt gacgggcgat gccgttggcc tcatccacgt cgaagagcgc
     1201 cagaactgtt ttggtgccgc cgatgtcgcc tgcgagtaac ataaatcact ccccaattat
     1261 ggttatgttg tggatagccg gatcatcacg atgttggctg cggccggatc aggctcaggg
     1321 attgcgccag gccacgctcc aaacaatatg ccggtcgccc catcgttgag gaaagtatac
     1381 tgaggagttg tggcgcggga aagagttacg acgcgatatc gacatggccc gcggcgtcgg
     1441 cgtcttccag ggcatggatc agcgcgtcca gctcgtcgcg gcagtcggtg cagagcttca
     1501 ggtgatgttc aacgagttgt ttcctgtggc caaattcgtg gtgggcttcc aggcactcga
     1561 cgtattcgtc caggcagccg aagacttctt cgcacgatag ctcatcgtcg cgcgtctcgc
     1621 acagtaaacg catgagccgc tcaacgctct gctcatccag cgacgcgccg gcgcttgccc
     1681 ccgcgccttc cccagtgcgg ctccgcagct cccgaagttt ttcgatcagg ctagtcattc
     1741 tcatactttg acgaatcatg gcctatcatt cttacaacac ggccactacc ttccaacgaa
     1801 agaggcgagt atgtcctcag gtgtcaggtt gtcttcggcc agccgccgcc gcaggcgctg
     1861 gcgggcatca aagacgagct tgtagacggc attctgattg gagtccatca tccaggctac
     1921 ttcgctcaat ggccgcccct gaatgacggc cgcggtcaat gccgtgcgtt gcttctccgt
     1981 cagctcctca ttgatcaggt gattgacata ccccatcagc tccttccgct cggtcaactc
     2041 ctccgggcgc ggggccgggt cggccacaaa agagggcgtg aattccccct cctccgtctg
     2101 catcaaacca tcgagcgacg tgtcgcgcca gcgcttgcgg cgcaattccg tcaggccgat
     2161 gttgagcgcc accttgttgg cccaggttgt gaacaggctc cgcccggcga aggtgtcgag
     2221 gttttcgagg attttgagca gcgcgtcctg aatgaagtcg tcaatgagga cgtcgaactc
     2281 cggggccgac gtgtcgacct gcccgatgag gccgcgacgc aagccgttgg ccagcacgac
     2341 gcgcaagtcg gccagcgccg cctcacgtgc ggggccttca gcgcccaggt cggcgaccca
     2401 ctgttcgttg gtgcgtgcgt tcgtcgcgct cataacaaga ggtgtatcac ttctccggac
     2461 agacgtgatg tattgtaacc ctgaaatcat tatgtgccgc tacaaattca ggatcggggc
     2521 ctgcacacca ggtcatggca actcgataga tattttttta cgaagcgtga cgcgcggaaa
     2581 atcgccttcg cttgtatgta tttttgagtt atggtaagat gttcgcagca gaagagggga
     2641 tgtcgcctaa gagcggaacc cgatccaatg gcgaagtcat tccaatgacg ggtccattgc
     2701 ctgcggttgg gctgatgcga cggcccaacc ggcatggatc gcccggacag accgggcagt
     2761 cgggtgaacg ggttcgagaa aatccattcc gcgaggaaat tagcaggtag gtgacaaaaa
     2821 tggacatcat caaacaaaag cccggcttgg cggcgctggt agcattcatt gccggactca
     2881 tcatcggtct ggtcgtcctg ggttggggcc tcttccccgt ccaatacacc ggcgctgatc
     2941 cgtccggtct tttcccggag taccaggccg tcttcgtccg caacgcggcc gaactttatg
     3001 cgttcgacaa caaccaggaa aagctgcgcg gcgcgttgag cggctggggc ggcgacgccg
     3061 tcgcctgtga attggccaag agttcgattg acccggccga tcagcagcgc atgatcgccg
     3121 ccgcgacgat cgtcaatggc acgggctgcg gtagcctgac atcgccgggc acgcaagcaa
     3181 ccgtccccgt tgcgcctccc ggcgcaacgg ccgccctgcc cggcgcggaa tcgtccggcg
     3241 ggtccggctc actgccgacc atgctcctgc tgttgttgct ggccttgttg ctgctggcaa
     3301 ttttctacgt ctggaatcgt cgtcgtaccc tgatgcaggg gacagacgat gaagacgcgg
     3361 actacaccga aaacggcgtg agccgaaaat cggcccggcc gtcctatttc cgccccgccg
     3421 cggctgcgag cgcaaccgca accgcagccg agtcaagagc agtgcccatc gcccgcttcc
     3481 gcaccgccta cacccatggc cacgatacct atgacgattc gttcagcatc gagaacgcga
     3541 acggcgactt cctcggcgag tgcggcgtgg gcatctccga gacattgggc gcgtctgcat
     3601 ccaggaacgt gaccgcgttc gaagtgtggc tgttcgacaa gaacgacatc cgcaccatca
     3661 ccaaggtgat catgagcgat catgcctact tcgacgaggc attgaaagcc aagcttgccc
     3721 cgaagggcga gccggtgttg gcccgcgaga acgagaccat cgtgctggag acggcgacgc
     3781 tgatcatcaa cgccgagatc accgagatgg agtatggcga gagcggcgac atgcccgacg
     3841 ccagctactt cgaacgcttc accatcgagc tgtcggcctg ggccaaggaa ggcgattatg
     3901 ccgcttcgca gccggccggc gatgaggaag atttgctgaa ctattagaaa gagggatgca
     3961 atccgctggc cgcgcagatt atttttgcgc tgcttgagaa ttgcttaaag accaaccggt
     4021 cgccttgaag gaaagagagc acggtagcca aatggctacc gtgctctctt tataaatcga
     4081 tagtctgttg ttgagcaacc tatcctacgc tcgaatgtag ggatcatata gccgctgcca
     4141 atcgtcgagc gcgtagcgat cggtcatgcc ggcgatgtag tcggctacaa cccgctccat
     4201 cggggcggtc tccagccggg cctgcgtttc gtcaggcagc atgtgcggtt ccttgacata
     4261 agcatcaaat aattcggcga cgaaacgttc ggccttggtc tgcatgcgca tcacccgata
     4321 gtgacgatac atccgatcaa agaggaactt tttgagcgct cgcacctgtt ggccgaaatc
     4381 cggcgtataa cccacgacgt tttccgggtg acgctggagc ttctcggggg aatcaatatc
     4441 gttggcgtct aatgcctctg cggtgcgggt catcacgtcg ccgatggaac ggccgatcag
     4501 ctcgcggata atctcatgcc ggtgcatcga tgtgaaatgc tcgcccggct tccaacccgc
     4561 gacttcgcgc agttcgcgcc agatggccag atcgtccagg tcggtcaacg taaacagccc
     4621 ggcgcgcagc ccgtcgtcca ggtcgtgggc attataggcg atctcgtcgg cgaagttggc
     4681 gatctgcgcc tccagcgagg cccgtttatt cggctcatat tcggccgcct cgctccggtc
     4741 gtaatcggtc tcgtgcttca gcatcccctc gcgggtctca taggtcagat tcaggccggg
     4801 gaaatccgga tagctatcct ccagctttgt gacgatgcga tagctctgtg tgttgtggtt
     4861 gaatccgccg tgatcggcca tcaggatatt gagcatgtgc tcgccggcgt ggccaaacgg
     4921 gggatgtccc agatcgtgcg ccagacagat cgcctcagtc aggtctacat tgccacccag
     4981 cccgcgggcc agcgagcggc cgagttgcgc gacttcaagc gtgtgggtca ggcgcgtgcg
     5041 ataatgatcc ccctcaaaaa agacgaaaac ctgcgtcttg tacatgagcc gacgaaaggg
     5101 ggtggtgtgg atgatgcgat cccggtcacg ggtgaaggcg gggcgcgtgg ccgattccgg
     5161 ttcgggataa gcccggccgc gcgactctgc gctgcgcaat ccatagggcg cgaggctcat
     5221 cgactcgatc tcctccagtt gcggccgttt acgtatcatg ggttctctct cctcggttgt
     5281 gggctaaaca gtggagttcg gcgcaagcga tccggacagg gggttgccac cgaacaccag
     5341 caagtgtaac ataatcatat cctgaccgct atcg
//
LOCUS       DAOMQG010000233         5366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6454_length_5366_cov_10.371305, whole genome shotgun sequence.
ACCESSION   DAOMQG010000233 DAOMQG010000000
VERSION     DAOMQG010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6454_length_5366_cov_10.371305"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="PLC06_11850"
     CDS             <1..1663
                     /locus_tag="PLC06_11850"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91183.1"
                     /translation="GGKVTEFYGLTEIDDKGLVVTIEEPTGSLPPPVFIDPPADMVEM
                     AAYFEALEGMRVAYEGGVLVAGPTHEGCGFAVIAEAAAAELPAIRRADDDPVGRVVPV
                     LYPGDLSCDDIPQVKTGDRITGLAGALTYNFDQFKIIFDAPAKLVVEFVPSPSLPELP
                     ETGPEQIVVASINAENYFDTTRDTDEEGEPVLTDDELAARQAKLAHLIADALECPTLI
                     GLQEVEHEALLRELSAALEAPCGFTYQISHLESPDARGIDNALLSDPRRVRVDAVSLR
                     QACSPVPTEIDDLSVVCAPGDEPLFGRPPLQVDALIDGSSFTLFVNHFKSKRGGEIET
                     GLERIRQAVVLNGLAAELLAVDPAARIVALGDFNDTDRSPVLALLADSAQGGVFANAL
                     AFVPQAARYSYNFGGVSELIDEILVSPSLVGEVAWATVVHVNTDYPVGWRLDTSPERL
                     PFRASDHDVPLVYLGQWPPTPVPEPTARATPVTRQQKTAIAPAPEVAPTKIATPSPSP
                     AATHEATAIIELPADVTPSRPSVWLLVPIGAAVLLLLALWYRARR"
     gene            1902..2300
                     /locus_tag="PLC06_11855"
     CDS             1902..2300
                     /locus_tag="PLC06_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91184.1"
                     /translation="MTDIQATPPASDVDPDVLRNLLQRIPLGAAQTILITRGGQLLTF
                     RGALKQVEAQDVAMHVDQDWRDTGQTMRVQFMRLPLSPLSRLLVVHPLRDTYRLILVD
                     SEDASLEPLRKLSNQLLGVLDVAGIGQKRV"
     gene            2441..>2676
                     /locus_tag="PLC06_11860"
     CDS             2441..>2676
                     /locus_tag="PLC06_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91185.1"
                     /translation="MTTKKLVLIDGHALAYRMFFALPLEAFTTKDGEPTNATYGFTRT
                     LLELILSPEPPEYLAVSFDTGATFRDALFTEYKGT"
     assembly_gap    2677..2776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2777..5014
                     /gene="polA"
                     /locus_tag="PLC06_11865"
     CDS             <2777..5014
                     /gene="polA"
                     /locus_tag="PLC06_11865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRO91186.1"
                     /translation="SDNIPGVKGIGEKTAIKLLAEYDTLDNLYEHLDEIKGALRQKLE
                     DGRPAADLSYKLARIVTDAPIRVELKDCLTHDYDVTQVLDIFRDLEFRSLSRMLAAST
                     ETSDLPEPPAGEGDATWQPTEVVVVRDEATLKEMAARMNAARWISFDLETNSLERLSA
                     GIVGFCLAVEPPIAYYVPVGHLAGAAQADSGQMNLFAGAAELVPGQLPMGRVLEAVRP
                     ALTNPDIPKVAHNAKFDCMILERYDITVSPVRFDTMIAEWLSDPATKHKGLKDLARHR
                     LGAEMTDIDELIGRGKNQTSFAEVPIEAAAPYGAADADMTLRLLPPLQHELEEKGLTK
                     LMDLEMELLPVLSDMERIGVRIDIEFFRHMSQDLAAALLKCEQTIYEIAGEPFNINST
                     QQLSDILFKKLQLPREGLKKIASGHYSTAADVLEGLKAADTTGIIDAIIEYRELGKLK
                     STYVDALPQMINPDTGRIHTSFSQTGAITGRLASSSPNLQNIPIRSEVGQLIRRGFVT
                     DPGWTFLSADYSQVELRILAHISQDETLLRAFREDQDIHTATAAAIYGIPPETVTKNQ
                     RRFAKVINFGLIYGMGAFRLARETGLTLSEAENYVKRYFGQFPGIERYLNATRQKART
                     DGYVETLFGRRRYFPVFRTSAGSANRQAVQRAEREAVNQPIQGTAADIIKVAMIRLHA
                     ELAAHYRARLLLQVHDELLLEVPEEELDAVRELVVETMSNAFTLDVPLKVEAAIGANW
                     LELKE"
     gene            complement(5020..>5366)
                     /locus_tag="PLC06_11870"
     CDS             complement(5020..>5366)
                     /locus_tag="PLC06_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91187.1"
                     /translation="SGYGLTLGDNDGRLLVALSPLGYVTVREERKDAEPVAYMPWQTW
                     THVQSGTATNEIWLDVDNRDDQAAVTVRINRELLWQGKIHVTPQQVGLYQTTFGGPAA
                     VDFRQVEWFAEP"
BASE COUNT         1009 a         1594 c         1630 g         1033 t
ORIGIN      
        1 cggcgggaag gtgacggagt tctatgggct gacggaaatc gacgacaaag ggctggtcgt
       61 gacgatcgag gagccaaccg gctcattgcc tccgcccgtc ttcatcgatc cgcccgccga
      121 tatggtcgag atggccgcct atttcgaagc gctggaagga atgcgcgtgg cttatgaggg
      181 tggtgtgctc gttgccgggc cgacgcacga ggggtgtggt tttgcggtta tcgctgaagc
      241 ggcggcggct gaactgcccg caattcggcg cgcggacgac gatccggtcg gccgcgttgt
      301 ccccgtgctc taccccggcg acctgagttg cgacgacatc ccccaggtga agaccggcga
      361 tcgcatcacc gggctggccg gggcactgac ctataatttc gaccagttca agatcatctt
      421 cgacgcgcct gccaagttgg ttgtggagtt tgtgccgtcg ccgtcgttgc cggaattacc
      481 ggaaacgggg ccggaacaga tcgttgtggc gtcgatcaac gccgagaact attttgacac
      541 gacacgggac actgatgaag agggtgagcc ggtcctgacc gatgatgaac tggccgctcg
      601 ccaggccaag cttgcccatc tgatcgccga cgcgctggag tgcccgacgc tcatcggctt
      661 gcaggaagtg gaacatgagg cattgttacg cgagttgtcg gccgcactgg aagcgccttg
      721 tggttttacc tatcagatca gtcacctgga aagccccgat gcgcgtggca tcgataacgc
      781 gctgctgagc gacccgcgtc gggtgcgcgt cgacgcggtt tccctgcgac aggcctgctc
      841 gcccgtgccc acggagattg acgatctgtc cgtggtttgc gcgccgggcg atgagccgct
      901 cttcgggcgg ccgccgctac aagtcgacgc gctcatcgac gggtcttcct tcacgctctt
      961 tgtgaaccat ttcaagtcga agcgaggcgg agagatcgag acgggcctgg agcgtattcg
     1021 gcaggcggtc gtcctgaatg ggttggcggc cgaactattg gcggtcgatc cggcggcgcg
     1081 gatagtcgcc ctgggcgact tcaacgacac cgaccgttcc cctgtgcttg ccctgctggc
     1141 cgactccgcg caaggtggcg tgtttgccaa tgcgttggcg ttcgttcctc aggcggcgcg
     1201 ttatagctat aacttcggcg gcgtgagtga gctgatcgac gaaatcctgg tatcgccgtc
     1261 gctggtgggc gaggtcgcct gggcgacggt ggttcacgtc aacacggatt atcccgtcgg
     1321 ctggcggctg gatacgtcac cggaacggct gccgtttcga gcgtcggatc atgatgtgcc
     1381 gttggtttac ctgggccaat ggccgccgac gcctgtgccg gaaccaaccg ccagggcgac
     1441 tcctgtcacc cgacaacaaa agacggccat agcgccggca cctgaagttg ccccaacaaa
     1501 gatcgcaacc ccttcgccat caccggccgc tactcatgaa gcaactgcca tcatcgaatt
     1561 accggccgat gtaactccat ctcgcccctc agtctggctc cttgttccga tcggcgcggc
     1621 cgtgctgctc cttcttgccc tgtggtatcg ggcaagacga tagctcggcg accgttgacg
     1681 gccgaccgcc gaccacggca agagcggttg cacagagacg acagacgaca gacgacagac
     1741 cacggcaaga gcgcgcctat cgtacttcgc cgttctttgc cgttcgcact tcgttatcca
     1801 tcatcttcac tcgcaactcg tcactctttt ttacgcttcc tgcatggcat caccgtgtcg
     1861 gccggatatc ctctataatg gagtgagtag aggcacaagc cgtgactgat atacaggcaa
     1921 ccccacccgc gagcgacgta gaccccgacg tgttgcgcaa tctgttgcag cgtatccccc
     1981 tgggtgcagc gcagaccatc ctcatcacgc gcggcgggca gctcctcact tttcgtggcg
     2041 cgctgaagca ggttgaggcg caggatgtcg ccatgcatgt ggatcaggat tggcgcgata
     2101 ccggccagac catgcgcgtg cagttcatgc gcctgcccct gtcccctctc tcccgtttgc
     2161 tcgtcgtcca ccccttgcgc gatacctatc ggctcatcct cgtcgatagc gaagatgcct
     2221 cgctggaacc gctgcgcaag ctgagcaacc agttattggg cgtgctcgat gtcgccggta
     2281 tcggccaaaa acgggtataa tacgcttcct gatccgccgc ttgtcggctt ccgatttgct
     2341 ttccccgact gtttgatttg cgcggccagg tggtgacttg ccgtcgtggt agtgggctga
     2401 gagaacgcaa aaccacgtgt ctgcatccta aggaactgtt atgaccacga agaaactggt
     2461 gctcatcgac ggccacgcgc tggcctatcg catgtttttt gctctgccgc tcgaggcgtt
     2521 caccaccaag gatggcgagc caaccaacgc gacgtatggc ttcacacgca ccttgctgga
     2581 actcatcctg tcccccgagc cgccggagta tctggccgtc agcttcgaca cgggcgcgac
     2641 ctttcgtgac gcactgttca cggagtataa agggacnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnagcg acaacatccc cggcgtgaag gggatcggcg agaagacggc
     2821 catcaagctg ctggctgaat atgacacgct ggataacctt tacgagcacc tggacgagat
     2881 caagggcgcg ctccgccaaa agctggaaga cggccgcccg gcggccgatc tcagctacaa
     2941 actggcccga attgtgaccg acgcgccaat ccgcgtcgag ttgaaggatt gcctgaccca
     3001 cgactacgac gtcacccagg tactggatat cttccgtgat ctggagttcc gttcgttgtc
     3061 gcggatgctg gcggcgagca cggaaacgag cgatcttcct gaaccgccgg cgggcgaagg
     3121 cgatgcgacg tggcagccta cggaggtggt cgtcgtgcgc gacgaagcca ccctgaagga
     3181 gatggccgcg cgcatgaacg cggctcgatg gatcagcttc gatctggaga ccaacagcct
     3241 ggagcgtttg tcggccggca tcgtcggttt ctgcctggcg gtggagccgc cgatagccta
     3301 ctacgtacct gtcggccatc tcgccggcgc ggctcaggct gattcgggac agatgaacct
     3361 gtttgccggc gcggccgagc tggttccagg gcaattgccc atggggcgcg tgctggaggc
     3421 tgttcgtccg gcattgacca atcccgacat ccccaaggtg gcccacaacg ccaagttcga
     3481 ttgcatgatt ctggaacggt atgacatcac tgtctcgccg gtcaggttcg atacgatgat
     3541 cgccgaatgg ctttcggacc cggctacgaa gcataagggg ctgaaggatt tggctcgtca
     3601 tcgcctgggc gcggagatga ccgacatcga tgagctgatc ggccgtggca agaatcaaac
     3661 ctcattcgcc gaggtgccga ttgaagcggc cgccccctat ggcgcggccg acgccgatat
     3721 gaccctgcgc cttctgccgc cattacagca cgaactggag gagaaggggc tgacgaagct
     3781 catggacctg gagatggaat tgctgccggt gctctccgac atggagcgca tcggtgtgcg
     3841 cattgacatc gagttctttc gtcatatgtc gcaggacctg gccgcggccc tgctcaagtg
     3901 cgagcaaacg atctacgaga tcgccgggga accgttcaat atcaactcga cgcagcagtt
     3961 gagcgacatt ctgttcaaga aactgcaact gccgcgcgag ggactgaaga agatcgccag
     4021 cggccattac tccacggcgg ccgatgtgct ggaggggctg aaggcagccg atacgacggg
     4081 catcatcgac gccatcatcg agtatcgcga attgggcaag ctaaagagca cctatgtcga
     4141 cgcgctgccg cagatgatca accccgacac aggtcgcatc cacaccagtt ttagccagac
     4201 tggcgcgatc accgggcggc tggccagcag cagccccaac ttgcagaata tccccattcg
     4261 cagcgaggtg ggccagctta tccggcgcgg cttcgtcacc gatccgggct ggacgttcct
     4321 gtcggccgac tattcgcagg tggagttgcg catcctggct catatcagcc aggacgagac
     4381 gctgctgcgc gccttccgcg aagaccagga tatccacacc gccacggccg cggctatcta
     4441 tgggatcccg cctgaaaccg tgaccaagaa ccagcgccgg ttcgccaaag tgatcaattt
     4501 tggcctcatc tatgggatgg gggcgtttcg gctggcgcgg gagacggggc tgacgttgag
     4561 cgaagctgag aattatgtca agagatattt cggccagttc cccggcattg agcgctatct
     4621 gaatgccacg cggcagaagg cgcgcaccga cggctatgtg gagacgctct ttggccgccg
     4681 ccgctacttc ccggtgttca ggacgagcgc ggggagcgcc aatcggcagg cggtgcagcg
     4741 cgccgaacgc gaagcagtga atcagcccat tcaggggacg gcagccgaca tcatcaaggt
     4801 cgccatgatc cgcctgcacg ccgagttggc ggcgcattat cgcgcccggc tgctgctcca
     4861 ggtgcatgat gagctgctgc tggaggtgcc tgaggaggag ttggacgcgg tgcgggaatt
     4921 ggtggttgag acgatgagca atgcgtttac gctcgatgtg ccactgaagg tggaagcggc
     4981 catcggcgcg aattggctgg agttaaagga gtagcggcgc tacggctcgg caaaccattc
     5041 cacctggcga aaatcaacgg ccgccggccc gccgaaagtt gtctgataaa gcccaacttg
     5101 ctgtggcgtg acgtggattt tcccctgcca cagtaattcg cgattgatcc gaacggtgac
     5161 ggccgcctga tcgtcacgat tgtccacatc cagccaaatc tcattagtgg ccgtccccga
     5221 ctggacgtga gtccaggtct gccacggcat ataagcaaca ggttccgcgt cctttctttc
     5281 ttcccgcacc gtcacatagc cgagaggcga tagcgccacg agcagccggc cgtcgttgtc
     5341 gccgagcgtc agcccgtagc cgctgt
//
LOCUS       DAOMQG010000234         5310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6534_length_5310_cov_8.904282, whole genome shotgun sequence.
ACCESSION   DAOMQG010000234 DAOMQG010000000
VERSION     DAOMQG010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6534_length_5310_cov_8.904282"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLC06_11875"
     CDS             <1..837
                     /locus_tag="PLC06_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO91188.1"
                     /translation="ERKSLPSTLYTEEYFLTACEGYDVFIESEGAHLSRRLRDAFALA
                     DVQPGMRLLDVGCGRGEILRHCMRLGVEAHGMDYAVAAARMSRDVVAAEATRAAETER
                     AAAEGESLASLPGVLQADAKTLPFPSNYFDRALMFDVVEHLFPWELHQAYLEIARVLK
                     PGGRLVIHTAPNRWYDAYAYPWVRRVRILMGQGDAYPKDPRAITPVNQDVHVNEQDLI
                     RMRSGLKQAGYRDIKVWLDSPPQNRDESAIMAGLRRLAFDVPPFRWFFEREVFAVATP
                     GK"
     gene            834..3020
                     /locus_tag="PLC06_11880"
     CDS             834..3020
                     /locus_tag="PLC06_11880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO91189.1"
                     /translation="MTIGEQGRKEERGKGSGGAPLLLRSPAPLLLIILLIAFALRAWN
                     LNGIPPGLTGIPPGLTHDEANHGREAIGVLNGVYLYFFPLNYGSEPLYSYTVALFMAL
                     LGRGAFALRLVNVVFGTLTIVMTYAWASPRLGRTTALLAVALMAVSFWPLASSREALR
                     VGMLPFFMVLAVIAFWRILETDDGRPQTTDHRPQTPRVVVRRPWSVVLFGLAVALTLH
                     IYLAARVSWLLFPAFLAYLALFHRPVFRQSWRPIVAGLLLAGALIAPMFLYLRAHPEM
                     QTRLDMFDGPLGAVRAGNFVPILLNVRDALLAFVWPGFGDQFLAYNIPGRPVLDAVSA
                     AFFVAGLLVCLWRWRRPIHAFLLLWFLVGIIPSLVTGPTANTTRNMAALPAVYLIVAV
                     GFVVFVEWLADRITNYELRITNEERAPNSYLVSRISYLGLSFVILFVVWVAFVSGRDY
                     FVRWGQSAEVRGAYQHTLVESIRHVTANYSDAEPILFSTVYPGPAHDSSIALVMAADH
                     PPVSETARWADARYALVLPPERTLAVVPYSTPPHPAFISLLEAIETVDLRPTDLDPRF
                     TLYWLDGPTAAATLSAGSALAQPVDFNGAIELVAARWLAESARPGETAELLTVWRVLD
                     STRAGPVVPPSFTTDAVMFTHVLDGPVHDGAGGNLTQSDRLDAPSWAWRTGDVIAQIH
                     PVVVPESAAAGEYAAVVGIYDRTSEVRLPVVGGGDTAVVPPLVVVP"
     gene            complement(3094..>3481)
                     /locus_tag="PLC06_11885"
     CDS             complement(3094..>3481)
                     /locus_tag="PLC06_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO91190.1"
                     /translation="RYAVNGDDILLDTNAIIYFLEGRSRIAEHVLFAERIYYSVITEI
                     ELLSSPHLSEKDNEAIRGFLSRCLRADLTSDVVERAIHIRRTEGLKTPDAIIAATAIV
                     NGFPLVTADKRFGRVVDLRVITDILE"
     assembly_gap    3482..3581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3616..3822)
                     /locus_tag="PLC06_11890"
     CDS             complement(3616..3822)
                     /locus_tag="PLC06_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91191.1"
                     /translation="MLTLEIHDRALIEKLTELLQKRFGGDSERMVAELVRLYDDRLSR
                     LDYSGRIQWPSDALQYQREVRSEW"
     gene            4000..4962
                     /locus_tag="PLC06_11895"
     CDS             4000..4962
                     /locus_tag="PLC06_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRO91192.1"
                     /translation="MSRKRSPEEMSAAELEQLLYARRRAEREQRLRRAQADGRIVIPD
                     RETDESDPPDEARIEASLVAPRRSFWGWLADRGLLLVEIAAAVALLVIIGSLWSTNRQ
                     LNRELAEAQAAQGQALALPTATATPAIGVALLPGGHHPPVDGQAPEPGEADGIPEHLL
                     PIVNAYIPPPIPTPGPETARRLEIPALSGDYPIVQGDDWEQLKKGIGQYAGSGQPGKA
                     GNVVLSGHNDIYGEPFRYLDRLKPGDEIIVSTERQTYTYLVEEVRVVEPTDVWVMGPT
                     DFARVTLISCYPYRVNTRRIVVFGRLADNATTLESGDGTIGRLD"
     gene            5034..>5310
                     /locus_tag="PLC06_11900"
     CDS             5034..>5310
                     /locus_tag="PLC06_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91193.1"
                     /translation="MTKKYKPKPKSASRPAAQVTEVTEAVEETAAPTEAPQPRSTRAP
                     KLSRSQLAAERIEDEYAYITDDLRRVFILAGLIFALLIALNLVFQAMG"
BASE COUNT          889 a         1657 c         1642 g         1022 t
ORIGIN      
        1 gaaagaaaat ccctgccgtc cacgctctat acggaagagt acttcctgac ggcatgcgag
       61 ggatacgacg ttttcatcga atccgaggga gcgcatctgt cgcgacgatt gcgcgacgcc
      121 ttcgcgttgg ccgacgtgca accgggcatg cgtctgctcg acgtgggctg cggccgcggc
      181 gagattctgc gtcattgcat gcggctcggc gtggaggccc acggcatgga ctacgccgtc
      241 gccgccgcgc gaatgtcgcg cgatgtggtg gccgcggaag ccacgcgggc cgccgagacg
      301 gagcgggccg cggccgaagg ggagtcattg gcctctctgc cgggcgtgct gcaggccgac
      361 gcgaaaaccc tgcccttccc cagcaactat ttcgatcggg cgctgatgtt cgacgtggtg
      421 gagcatctgt tcccatggga gttgcatcag gcctatctgg agatcgcccg cgtgctgaag
      481 ccgggcggcc gtctggtcat ccacaccgcc cccaatcgct ggtacgacgc ctacgcctac
      541 ccatgggtgc ggcgcgtgcg catcttgatg ggccagggtg atgcctaccc caaagaccct
      601 cgcgccatca cgccagtcaa tcaggacgtc cacgtcaacg agcaggacct gatccgtatg
      661 cggagcgggc tgaagcaggc cggatatcgc gatattaagg tctggctcga ttcgccgccg
      721 caaaaccggg atgagtcggc aatcatggcc gggttacgcc ggttggcctt cgacgtaccg
      781 ccgttccgct ggttcttcga gcgggaggtg tttgcggtgg cgacccccgg aaaatgacga
      841 ttggagagca ggggagaaag gaagaaaggg ggaaagggag cgggggtgcg ccccttctcc
      901 tccgctcccc tgctcccctg ctcctcatca ttctcctcat cgccttcgcc ctgcgcgcct
      961 ggaacctgaa cggcatcccg ccggggctga ccggcattcc tcctggactg acccatgacg
     1021 aggccaacca cggccgcgag gccatcggcg tcctcaacgg cgtctacctc tatttcttcc
     1081 cgctcaacta cggcagtgag ccgctctata gctacaccgt ggccttgttc atggccttgc
     1141 tgggcagggg ggcgttcgcg ctgcggctgg tcaacgtcgt ctttggcacg ttgaccatcg
     1201 ttatgaccta tgcctgggct tcaccgcggc ttggccggac tacggctctg ctggccgtgg
     1261 cgctgatggc cgtctccttc tggccgttgg cctccagccg cgaggcgctg cgggtgggga
     1321 tgctgccgtt tttcatggtg ctggcggtga tcgctttctg gcgaattctg gagacagatg
     1381 acggccggcc gcagaccaca gaccacagac cacagacccc aagagtcgtc gtccgtcgtc
     1441 cgtggtctgt cgtcctcttc ggtctcgccg tcgccctgac actccatatc tatctggcgg
     1501 cgcgggtgag ctggttgctg ttcccggcgt ttctggcgta tctggcgctg ttccaccggc
     1561 cggtgttccg gcagtcatgg cggccgattg tggccggatt gctgctggcc ggggcgttga
     1621 tcgcgccgat gttcctttat ctgcgcgccc acccggagat gcagacgcgg ctggatatgt
     1681 tcgatgggcc gctgggggcc gtcagggcgg gcaatttcgt ccccatcctg ctgaatgtcc
     1741 gcgacgcgtt actggctttc gtctggcccg gcttcggcga ccaatttctg gcctacaaca
     1801 tccccggccg gccggtgctg gatgccgtct cggccgcgtt tttcgtggcc ggactgctcg
     1861 tctgtctgtg gcgttggcgg cggccgatcc atgccttcct gctgctgtgg tttctcgtcg
     1921 gcatcatccc ctcgctcgtc accggcccaa cggccaacac gacccgcaac atggcggcgc
     1981 tcccggcggt gtatttgatt gtggcggtgg gttttgttgt ttttgtagaa tggctggcgg
     2041 atcgaattac gaattacgaa ttacgaatta cgaatgaaga gcgcgctccg aactcgtatc
     2101 tcgtatctcg tatctcgtat ctcggcttgt cattcgtcat tttatttgtc gtctgggtcg
     2161 ccttcgtctc cggccgcgac tacttcgtcc gttggggcca gtcggccgag gtgcgcggcg
     2221 cctatcagca cacactggtc gagtcgatcc ggcatgtgac ggccaactat tccgacgccg
     2281 agccaattct cttctcgaca gtctaccccg gcccggccca cgattcatcc atcgccctgg
     2341 tcatggccgc cgatcatccg cccgtcagcg agacggcgcg ctgggccgac gcgcgatacg
     2401 cgctggttct gccgccggag cggacgctgg ccgtcgtccc ctactccact ccgccccacc
     2461 cggcgtttat ctccctgctg gaggccatcg agacggtcga tctgcggccg accgacctcg
     2521 atccgcgttt cacgctctat tggctcgacg gcccgacggc ggccgcgaca ctatcggccg
     2581 ggagcgcgtt ggcccaaccg gtcgatttca acggcgcgat agagcttgtc gccgcgcgct
     2641 ggctggcgga gagcgcccgc ccgggcgaga cggccgaact gctgaccgtc tggcgggtgc
     2701 tcgactcgac ccgcgccggg ccagtcgtgc cgcccagctt caccaccgac gcggtgatgt
     2761 tcacccacgt attggacggt cccgtccacg atggcgcggg cggcaacctg acgcagagcg
     2821 accggctcga cgcgccgtcg tgggcgtggc gaacggggga cgtgatcgcc cagattcacc
     2881 cggtggtcgt gccggagtcg gccgcggcgg gggagtatgc ggccgtggtg ggtatctatg
     2941 atcggacatc agaggtgcgg ttgccggtgg tcgggggcgg ggatacggcc gtggtgccgc
     3001 cgttggtggt tgtgccgtag aaataaccgg caatcgggta actacgccga aggctctgca
     3061 ccaggaatgt tcagcctata cgtttgatgc gcactattca aggatatcgg tgataactct
     3121 cagatcaacg acccggccaa atcgcttatc ggccgtgacc aagggaaagc cattgacgat
     3181 ggccgtagcg gcgatgatcg cgtcaggcgt ttttaggcct tcggttcgac ggatgtgaat
     3241 cgctcgctca acaacgtccg aagttaagtc tgcccgcaag cagcgggaga gaaatccccg
     3301 aatagcttcg ttatcctttt cggataaatg gggagaggat agcaactcga tttcggtgat
     3361 aaccgagtag tagattctct cggcaaacag gacgtgctcc gcgattcgag atcgcccctc
     3421 aaggaagtag atgatcgcgt tggtgtcgag caggatgtcg tcaccattca ctgcgtacct
     3481 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtagatgat cgcgttggtg
     3601 tcgagcagga tgtcgtcacc attcactgcg tacctcacgt tgatattgga gggcatcgga
     3661 aggccactgg attcggccgc tgtaatcgag tcgggacaga cggtcgtcgt agagtcgcac
     3721 caactcggcc accatgcgtt cggaatcgcc gccgaatcgt ttctggagca attcggtgag
     3781 tttttcgatt aaagcgcggt cgtggatttc aagagtcaac atcgcgattg ttctccgttg
     3841 gtgtcgatag tataccgcaa gtcgatctca aacagaagcc ccgcacagtc agactacagg
     3901 atgcagcctc tcattgcacc ctcactatcg aagtttttat gttaatttaa gcttatcttc
     3961 cttggaacag gcccccgtct gcgctatgat cgccctctga tgtcccgcaa gagatcgccc
     4021 gaagagatga gcgccgccga gctggagcaa ctgctctacg cgcgccgccg cgccgagcgc
     4081 gagcaacggc tgcgacgggc gcaggccgat ggccgcatcg tcatccccga ccgcgagacc
     4141 gacgagagcg acccgcccga cgaagcgcgg atcgaggcgt cgctggtcgc gccccggcgc
     4201 tcgttttggg gctggctggc cgaccggggg ctgctgctgg tggagatcgc cgccgcggtg
     4261 gcgctgctgg tcatcatcgg cagcctgtgg tcgaccaatc gacagctcaa ccgggagctg
     4321 gccgaggccc aagcggcaca aggtcaggcg ctggccctgc ccacggccac ggccacaccg
     4381 gccatcggcg ttgccctgct gccgggcggc caccaccccc cggtcgacgg tcaggccccc
     4441 gaaccgggcg aagccgacgg catccccgaa catctgctgc ccatcgtcaa cgcctacatc
     4501 ccgccgccca tccccacacc cggccccgag acagcgcggc ggctggagat accggccctc
     4561 agcggcgact accccatcgt ccagggcgac gactgggagc agctcaaaaa aggcatcggc
     4621 cagtatgccg gcagcggcca accgggcaag gcaggcaatg tcgtgctgtc gggccataat
     4681 gacatctacg gcgaaccgtt ccgctacctc gatcggctga agccgggcga cgagatcatc
     4741 gtctccacgg agcgacaaac ttacacctac cttgtcgaag aggtgcgcgt ggttgagccg
     4801 acggacgtgt gggtgatggg gccgacggac ttcgcgcggg tgacgctgat ctcctgctac
     4861 ccgtatcggg tcaacacgcg gcggatcgtg gtgttcggcc ggctggcgga caacgccaca
     4921 accctggaga gcggcgacgg aacgattggg cgcctcgact aaacgaaccg gctatggcgg
     4981 ccgcgcttct caggggctat acttattccc gatcgcaata tagaggacga gacatgacta
     5041 aaaagtacaa accgaaaccc aaatcggcat cccgccccgc cgcgcaggtg acggaagtca
     5101 ccgaagctgt cgaggagact gccgccccga ccgaggcgcc gcagccccgt tccacccgcg
     5161 cgcccaaact ctcgcgcagc caactggcgg ccgaacgtat cgaggatgaa tatgcctaca
     5221 tcactgacga tctgcggcgc gttttcatcc tggcggggtt gatcttcgcc ctgttgatcg
     5281 cgcttaatct ggtctttcaa gcgatgggtt
//
LOCUS       DAOMQG010000235         5287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6567_length_5287_cov_8.550268, whole genome shotgun sequence.
ACCESSION   DAOMQG010000235 DAOMQG010000000
VERSION     DAOMQG010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_5287_cov_8.550268"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLC06_11905"
     CDS             complement(<1..226)
                     /locus_tag="PLC06_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91194.1"
                     /translation="MKAEARAGKKRADGESDLLAKIAEMPEPERTMVTRLHEIIKGSA
                     PNLMPKTWYGMPAYANEAGKIVCFFQSAQKF"
     gene            complement(407..739)
                     /locus_tag="PLC06_11910"
     CDS             complement(407..739)
                     /locus_tag="PLC06_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO91195.1"
                     /translation="MNDPLSQTFAALADPTRRAMLERLAKGPATVKELAEPFQISMPA
                     ISKHLKVLEHAGLIERGREAQWRPARLQAAPLKDATQWLEAYRRNWEESFDRLEDYLR
                     RLQEKKSG"
     gene            951..1775
                     /locus_tag="PLC06_11915"
     CDS             951..1775
                     /locus_tag="PLC06_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91196.1"
                     /translation="MTINDENNTEPRDPFIIEMEPSDELTELVTSPPALLPGAAATEQ
                     QADPPAAADPEFDAYLAVVKLLIGGTIEGANELTKRLEQWEAAQRTAADAPPVGEINT
                     NGDVARYLLVGMALSTTDGLRRQALKLVQASDVFWRMTGSATQPLVNNRLTGIIAGPI
                     DRAIDRLALRGQKRVNDWIELGRTQEPNARRMARETYDNVFDEFIGLLAENQELADLV
                     QKKSMGLATEAVDEFRSRTVSADALAEKLVRRILRRPMREELLGSSGGGQVTAEGE"
     gene            1822..2418
                     /locus_tag="PLC06_11920"
     CDS             1822..2418
                     /locus_tag="PLC06_11920"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRO91197.1"
                     /translation="MTQLSNPLPSLRGFYAGFVTRSIAFAIDLILIAVTQITILFLVR
                     LLLDFFGLDQLARAIFEPTETTNVSVFITITRWVIAFIGSGFLFALYSISMWLLVDKT
                     LGQALLGLRVLRTDGRPLTLGPAIRRVLGYYISFLALFLGFLWVLVDDRRQGWHDKIA
                     DTIVVYDWEARLGKRMREWLARRQAEREAPPPDIFSQG"
     gene            2425..3837
                     /locus_tag="PLC06_11925"
     CDS             2425..3837
                     /locus_tag="PLC06_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HRO91198.1"
                     /translation="MSEAIVPLSNVDAAWLKMEDPTNLMMVSGVLTFAKPVDITYFRA
                     LIETRLLQFDRFRQRVVRPAFPMAPYYWEFDPTFNLNAHIHRIGLPHPHNKLALQELA
                     SYLMSTGLDFSKPLWQMHVVDGYSRDGYGEGGAIIVRLHHAMADGMALVGVLLALTEL
                     GPGAPPPEPDSLPEAGEPPNALVGSWEALQLRAARLMGKGVGVGRRALIEGLESYLNN
                     DRPRELAELSFDYAHAASKLVLRAPDPQTIYKGKLGVAKRAAWSRPLPMSEVKAMRRA
                     TGATLNDLMTAVVTGGLRRYMEARGEEIVDFRAAVPVNLRGPNEMGGMGNKFGLVFVE
                     LPVGTVDLSRRLALVRHRMESLKESQEAPVSLDILGAMGFSAQAIQDQVVKLIGTKAT
                     AVLTNVPGPPIPLYMAGQPIENIMFWVPQSGRLGLGISILSYAGNIYLGIATDAGLVP
                     DPDAIMLGFYAEYDDLLGLV"
     gene            complement(3847..4788)
                     /locus_tag="PLC06_11930"
     CDS             complement(3847..4788)
                     /locus_tag="PLC06_11930"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRO91199.1"
                     /translation="MTQSCIRVTHLNEVDHKQPTCVAIGSFDGVHLGHQALLRHMVAC
                     ARENGMRAAALTFFPHPRRVLRTLPPRFYLTTLNDRVRLLAELGIDLVITHPFDDEVR
                     HIRAADFIDRLIETLSMKQLWGGNFALGYQREGDVPFLRHQGELKGFTVETLSEPVLF
                     DGEIVSSRRVRAALEVGEMDTVTGCLGRYFCVRGPVVKGDQRGRTIGFPTANVAVWDE
                     LLLPANGVYATRIQVGDERLTAATNVGVRPTVDGMKLTVEAHILDFDADIYGEDVRLE
                     FVRRVRPEMKFAGLDALKAQIAADVEAVRQIVPDEGR"
     gene            complement(4906..>5287)
                     /gene="truB"
                     /locus_tag="PLC06_11935"
     CDS             complement(4906..>5287)
                     /gene="truB"
                     /locus_tag="PLC06_11935"
                     /inference="COORDINATES: protein motif:HMM:NF027523.2"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRO91200.1"
                     /translation="ACSSGTYIRSLAHDLGAALGCGGHVAALRRTAVNHFTLDDAVVL
                     DELTPENVAARLLPLEAIIAHLPRAEFDEAAAAELGFGRRVPADAGSPTGDTAAFGPD
                     GRLLGIVAVEEGSWRPKKMMSGVN"
BASE COUNT          946 a         1693 c         1626 g         1022 t
ORIGIN      
        1 cgaacttttg cgcactttgg aagaagcaga cgatcttgcc ggcctcattg gcatacgcgg
       61 gcatcccgta ccaggttttc ggcatgagat ttggcgcgct ccccttgatg atctcgtgaa
      121 gccgggtgac cattgtgcgc tccggttccg gcatctcggc gatctttgcc aacagatcgc
      181 tctctccgtc ggcccgcttc ttgccggcgc gcgcttcggc cttcaagttc gcgggcgcgc
      241 tcctgcatcg cggctttttt ctcgtcggta aagcccttgg cggtcttttg tgtttccttc
      301 ttcgtgctca ttctctcgat ctgctgttgt gtctgtcatc acgtcgtctc catccgacaa
      361 gaagtttaat ccgcggattt gaagaaggtt gtgcaattca ggagcttcag ccgctcttct
      421 tttcctgaag ccggcgcaga taatcctcca ggcggtcgaa gctttcttcc cagttgcggc
      481 ggtaagcttc cagccactgg gtcgcatctt tcagtggcgc ggcttgcagt cgcgcgggcc
      541 gccattgggc ctcgcggccg cgctcgatca gtccggcatg ctccagcacc ttgagatgtt
      601 tggagatcgc cggcatacta atctgaaacg gttcggccag ctccttcacc gtggccggcc
      661 ctttggccag ccgctccagc atggcccggc gggtcgggtc ggccaatgcg gcgaaggtct
      721 gactgagagg gtcgttcatg gtttgagcaa atacttaatt aactaattag ttaaatagta
      781 aaataaagta aacgaaatgt caagaatcaa agtgtattaa gccttgcgac aaggcaaccc
      841 tgtaatcttg tattcgatgc atgcctgtca tatacttttg actacgtggg agatgtcgct
      901 tcaatccggg ggttcgctcg aacgaaacgt catgcccaag cgtattgacc atgacgatca
      961 acgacgaaaa caacaccgag ccgcgggatc cttttatcat cgagatggag ccgtcggatg
     1021 agctgaccga actggtcacc tcgccgcctg ctctgctgcc cggcgcggcc gcgaccgagc
     1081 agcaggccga tccaccggcc gcggccgatc ctgaattcga cgcctatctg gccgtcgtca
     1141 agctgttgat cggcggcacc atcgagggcg cgaacgagct gaccaagcgg ctggagcagt
     1201 gggaagcggc acagcgaacg gccgccgatg ccccgcctgt aggcgagatc aacaccaacg
     1261 gcgatgtggc ccgctatctg ctggtcggca tggcgctctc gaccaccgac gggctgcgcc
     1321 ggcaagcgct caagctggtg caggcctccg atgtcttctg gcggatgacc ggcagcgcta
     1381 cccagccgct ggtcaacaat cgcctgaccg gcatcatcgc cgggccaatc gaccgtgcca
     1441 tcgatcggct ggcgctccgt ggccaaaagc gggtcaacga ctggatcgaa ttgggccgca
     1501 cgcaggagcc gaacgcgcga cgcatggccc gcgagacgta tgacaacgtc ttcgacgagt
     1561 tcatcggcct gctggccgaa aatcaggagt tggccgatct ggttcagaag aagagtatgg
     1621 ggttggcgac cgaagccgtg gatgagttcc gctcgcgaac tgtctcggcc gacgcgctgg
     1681 ccgagaagct cgtccggcgg atcctgcggc ggccgatgcg cgaggaactg cttggttcaa
     1741 gcgggggcgg ccaggttacg gccgaaggtg aatgaaaaac cgtcaacctg ttaaaccgat
     1801 tcattccatc aacaacgaac cgtgacccag ctcagcaacc ccctcccctc gcttcgcggc
     1861 ttttatgctg gattcgtcac tcgttccatc gctttcgcca tcgacctgat cctgatcgcc
     1921 gtcacccaga tcacgattct gttcctcgtg cgtctgctgc tggacttctt cggcctcgac
     1981 caattggccc gggcgatctt tgaaccgacc gagacgacca acgtgtcggt cttcatcacg
     2041 atcacgcgct gggtcatcgc cttcatcggc agtggtttcc tgttcgctct ctattccatt
     2101 tccatgtggt tgctggtcga caagacgctg gggcaggcgc tgctgggctt gcgggtgttg
     2161 cgcacggatg gccggccgct gacgcttggc cctgccatac ggcgcgtcct gggctattac
     2221 atctctttcc tggcgctctt cctcggcttt ttgtgggtat tggtcgatga tcgtcgccag
     2281 ggctggcatg acaaaatcgc cgacaccatc gttgtctacg actgggaagc gcgcctgggc
     2341 aagaggatgc gtgaatggct ggcccgccgc caggccgagc gcgaggctcc gccgcccgat
     2401 atattttctc aaggataaac agcaatgtct gaagccattg tccccctctc caatgtcgac
     2461 gccgcctggc tcaagatgga agacccgacc aacctgatga tggtgtcggg cgtcctgacc
     2521 ttcgccaagc cggtggacat cacctatttt cgggccttga tcgagactcg cctgcttcag
     2581 ttcgatcgct tccgccagcg cgtcgtgcgg ccggccttcc cgatggcccc ctactattgg
     2641 gagtttgacc cgaccttcaa cctgaacgcc cacatccacc gcatcggcct gccccatccc
     2701 cacaacaagc tggccctgca ggaactcgcc agctatctca tgagcacggg gctggacttc
     2761 agcaagccgc tgtggcagat gcacgtcgtg gacggctaca gccgtgacgg ctatggcgaa
     2821 ggcggggcga tcatcgttcg gctccatcat gccatggccg acgggatggc tctggtcggc
     2881 gtgttgctgg cgctgaccga gctaggcccc ggcgcgccac cgccggaacc ggatagcctg
     2941 ccggaagccg gcgagccgcc gaacgcgctg gtcggctcgt gggaagcgct gcaattgcgc
     3001 gcggcccggt tgatgggcaa gggcgtcggc gtcggccgcc gggcgctcat tgaaggcctg
     3061 gagagctatc tcaacaacga tcggccgcga gagctggccg agctatcgtt tgactacgcc
     3121 cacgccgcga gcaagctcgt tctgcgcgcc cccgacccgc aaacgatcta caagggcaag
     3181 ctcggcgttg ccaagcgcgc cgcctggtcg cggccgctgc ccatgagcga agtgaaggcc
     3241 atgcgccggg ccaccggcgc gacgctcaac gacctgatga cggctgtcgt caccggcggg
     3301 ctgcgccgct atatggaagc gcgcggcgag gaaatcgtcg acttccgcgc cgccgtgccg
     3361 gtcaacctgc gcgggccgaa cgagatgggc gggatgggca acaaatttgg gcttgtgttt
     3421 gtcgagttgc ccgtcggcac ggtcgacctg tcgcggcggc tggcgctcgt gcggcatcgc
     3481 atggagtcgc tgaaggagag ccaggaagcg ccggtgtcgc tcgacattct gggggcgatg
     3541 ggcttttcgg cccaggctat tcaggatcaa gtcgttaagc tcatcggcac caaggcgacg
     3601 gccgtgctga ccaacgtccc cggcccgcct atcccgctct atatggccgg gcagccgatc
     3661 gagaacatca tgttctgggt tccacagagc ggccggctgg gtctgggcat cagcatcctc
     3721 agctacgcag gcaacatcta tctcggcatc gccaccgacg cgggccttgt ccccgacccg
     3781 gacgcgatca tgctggggtt ctacgccgaa tatgatgatc tgttggggct ggtctaggcg
     3841 cgggcgtcat cgcccctcat ccggaacgat ttgcctgacc gcttccacat cggccgcaat
     3901 ctgcgccttt agcgcgtcca gcccggcgaa cttcatctcc gggcggacgc ggcgcacaaa
     3961 ctccagccgc acgtcttcgc cgtagatgtc ggcgtcgaag tcgaggatgt gagcctccac
     4021 cgtcagcttc atcccgtcca cggtcggccg cacgccgacg ttggtcgccg ccgtcaggcg
     4081 ctcgtcgccg acctggatgc gggtggcata gacgccgttg gccggcagca gcaactcgtc
     4141 ccacacggcc acgttggcgg tcgggaagcc gatcgtccga ccacgctgat cgcccttcac
     4201 caccggcccg cgaacgcaga aatagcggcc gaggcagccg gtgaccgtgt ccatctcgcc
     4261 cacttccagc gcggcgcgca cccgccggct gctgacgatc tcgccgtcga agagcaccgg
     4321 ctcgctcagc gtctccacag tgaaaccctt cagctccccc tgatggcgca ggaagggcac
     4381 gtctccttcg cgctggtagc cgagagcgaa gttgccgccc cacagctgct tcatggacag
     4441 cgtctcgatg agccggtcga tgaagtcggc ggcgcggatg tggcgcactt cgtcgtcgaa
     4501 ggggtgagtg atgaccagat caatgcccag ctcggccaac aggcgcacgc ggtcgttcag
     4561 cgtcgtcaga tagaagcgcg gcggcagcgt acgcaggacg cgacgcgggt gggggaagaa
     4621 ggtcagcgcg gcggcgcgca tgccattttc acgggcacat gccaccatgt gtcgcaatag
     4681 cgcctgatgg ccgagatgaa cgccgtcgaa cgagccgatg gcgacgcagg taggttgttt
     4741 gtgatcgacc tcgttgagat gggtgacgcg aatgcaggat tgggtcatag tttaattgca
     4801 gacaggggcc acgattcatg atgcggccgc cggcgggatt gtaacagagt tggggagaat
     4861 gcagtacatc cctgagttaa gtgcgactgt caagagcatc ctcggctagt taacgccgct
     4921 catcattttc ttcggccgcc acgatccttc ctcgacggcc acaatgccca gcaagcgccc
     4981 atccgggccg aacgcggccg tgtcaccggt ggggcttcca gcgtccgccg gcacgcgccg
     5041 cccaaaaccc aactcggccg cggccgcctc atcgaactcc gcccgcggca ggtgggcgat
     5101 gatcgcttcg agcggcagca agcgcgcggc gacgttttcc ggcgtcagct catccaaaac
     5161 caccgcatca tccagcgtga aatgattgac ggccgtccgg cgcagcgccg ccacatggcc
     5221 gccgcagccc aacgccgcgc ccaaatcgtg ggccagcgag cgaatgtagg tgcccgacga
     5281 gcaggcc
//
LOCUS       DAOMQG010000236         5283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6574_length_5283_cov_9.754591, whole genome shotgun sequence.
ACCESSION   DAOMQG010000236 DAOMQG010000000
VERSION     DAOMQG010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6574_length_5283_cov_9.754591"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLC06_11940"
     CDS             <1..290
                     /locus_tag="PLC06_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HRO91201.1"
                     /translation="KTAEAVASYLIEAIRRNIMAGTLTKIGGALARPAFKRVRDEFNP
                     DEVGGAPLMGVNGVVIIAHGRSNAYAVQQAIGQARRVVDGGIVAAIAEGVK"
     gene            406..1680
                     /gene="fabF"
                     /locus_tag="PLC06_11945"
     CDS             406..1680
                     /gene="fabF"
                     /locus_tag="PLC06_11945"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRO91202.1"
                     /translation="MSNETLDARGRPRVVITGMGMLSPLGNTVEESWENMMAGRSGIT
                     RITQFDASELPTQIAGEVKDFVARDYMDFKEARRMSRASQLAVAAARMAVADAGLPER
                     VPDSDRVGVLVGTAAGGMERAFEEIDTFHERGFRAVSPFGMTMFLCNMPTHHVSLTTG
                     STGPLSTIVAACATGTQTIGEGAEFIRRGAADMMFAGGVEGLIHSSAIVGFGAMRALS
                     THFNDAPERASRPFDKDRDGFILAEGAAVVVLERLDKALARGARIYGEVLGHASSSDA
                     YHVAAPDPEAKGAIRAMQWALDDAGLTPNDVDYINAHGTSTPVNDATETLAIKRLFGE
                     RAWQVPISSTKSAMGHAMGAAGTIESIFTLLGLGREIIPPTWNYETPDPECDLDYVPN
                     APRPAPIRIALKNSFGLGGQNACLVLGRYNNE"
     gene            1770..2513
                     /locus_tag="PLC06_11950"
     CDS             1770..2513
                     /locus_tag="PLC06_11950"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HRO91203.1"
                     /translation="MIILRDDKRIARLGRLAQILSFAGLAALVGGLIIIFVFDNQANV
                     FLYQLLALLVGFSLSQVGLHFAHRYLRQPRMDEVLDRAASKFARKDGRLYHYLLPAPH
                     VLLLPTGVFVLTAKFQNGRITANGDTWQQTGLGMRRFFGRESLGNPTREAEAQVARMA
                     EYIQQHAPAAAGAPLFPIIVFTAPNIASLDVKESRIPAVHAAKLSGALRQQSINLKPL
                     PKAQYDALRAAFDGTAAHLLETTVEGSEE"
     gene            2554..3300
                     /locus_tag="PLC06_11955"
     CDS             2554..3300
                     /locus_tag="PLC06_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0022611 - dormancy process [Evidence IEA];
                     GO_process: GO:0030643 - cellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:2000186 - negative regulation of phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HRO91204.1"
                     /translation="MATSVRALLDTELNELNQSVLTLSSMVDEAIGRAIEALLTNDAT
                     LAGNVIAGDAEINRLRFHIEEKAYIILSTQQPLARDLRTVIAAIHFAIELERIGDHAA
                     GIARIVQRLQETPQPVVFELAVPSEVTEIAEGSEVGAPVRLKLSRLPKMTKRARDMLH
                     RATEAYIERDVEKANAIVKRDRKIDNQYRKFFAEAMAEIANKHVGEIPTYLLWIAHNL
                     ERIGDRTTNLAERVVFMVTGQYTEVLEDYG"
     gene            3546..4844
                     /locus_tag="PLC06_11960"
     CDS             3546..4844
                     /locus_tag="PLC06_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRO91205.1"
                     /translation="MKRLSLLLVLLLLAALALVACQPAGGTTAPAEESAPAEEAAPAE
                     EEAAPAEEEAAAAFECTDAIGCVEVAAGDPIQIASALVISGANADLGLDSQRGVEIAL
                     KMRPEVLGHKVELIAEDDGCSAEGGQTAGTKIVANPQIVGAIGTSCSGAGVPFAQILS
                     DAGYATISPSNTSPALTDPNQAFKIGYLRTAHNDLIQGAAMADYAYDVRGFTKAAAIH
                     DGDPYTEGLAKAFADAFVAKGGEMVAFEAEAADATNVEPLLTTIAAAKPEFLYFPVFI
                     PLGSLIVNTAKTIPGLESVQLAAADGVQSPSFLENVVGTGEGMVVSGPDLAFSNTFYK
                     DEFLPMYEADYGTAPTAPFHAHSFDATNMLLNAIEKVAQQDADGNLLIGRQALRDALY
                     DTSGMEGITGTLTCSEFGDCADPKIAVSEIQSGEFVRIWP"
     gene            5012..>5283
                     /locus_tag="PLC06_11965"
     CDS             5012..>5283
                     /locus_tag="PLC06_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO91206.1"
                     /translation="MTSSRRLKDLDWIGLALWVFRAAIIILVVVGTVRKLFFGVGTQY
                     TGRDWLDFAVSGLSQGGLYALIALGYTMVYGVLFMINFAHGEFFMSG"
BASE COUNT         1044 a         1618 c         1595 g         1026 t
ORIGIN      
        1 tgaagacggc cgaggcagtc gcgagctatc tgatcgaagc cattcgcaga aacatcatgg
       61 ccggtacgct gaccaagatt ggcggcgcgt tggcccgccc ggccttcaag cgcgtgcgcg
      121 acgagttcaa ccccgatgaa gttggcggcg cgccacttat gggcgtcaac ggggttgtaa
      181 ttatcgccca tggccgctcg aacgcctatg ccgtccagca ggccatcggc caggcgcggc
      241 gggtggtcga tggcggcatc gtcgccgcga ttgccgaggg tgtgaagtag agaagaattc
      301 aacgcaatgg cgcgagaagg ggagagtaca aagtattagg ttctgcctat gtgccttggc
      361 gctcttgcgc cttgcgccag agatgtttcg cttgaaagga actttatgag taatgagacc
      421 ttggatgcgc gcgggcgacc acgcgttgtg ataaccggga tgggcatgct cagcccgctc
      481 ggcaataccg ttgaggagag ctgggaaaat atgatggccg ggcgctccgg catcacccgc
      541 atcacccaat tcgacgcgag cgaactgccc acacaaatcg ccggcgaggt gaaggacttc
      601 gtcgcccgtg attatatgga cttcaaggaa gcccgacgca tgtcgcgcgc ctcgcaactg
      661 gccgtggctg cggcgcggat ggcggtggcc gatgccgggc tacccgaacg ggtgcccgac
      721 tccgaccgcg tgggcgtgct ggtgggcacg gccgccggtg gtatggagcg cgcgtttgaa
      781 gagatcgaca ccttccatga acgcggcttt cgcgccgtca gcccgtttgg tatgacgatg
      841 ttcctgtgta atatgcccac ccaccacgtg agcctgacga ccggctcaac tggcccgctc
      901 agcaccatcg tagccgcctg tgccacaggc acgcagacca ttggcgaggg agccgagttc
      961 attcgtcgcg gtgcggccga catgatgttt gccggtggcg tcgagggcct gatccatagc
     1021 tcggccatcg tcggttttgg ggccatgcgc gccctgtcta ctcatttcaa tgacgctccc
     1081 gaacgcgcca gccggccgtt cgacaaggat cgcgacggct tcattttggc tgaaggcgcg
     1141 gccgtcgtcg ttttggagcg gctcgacaag gcgctggccc gcggcgcgcg catctatggc
     1201 gaggtgcttg gtcatgcctc atcgtccgac gcctatcacg ttgccgcgcc cgaccctgag
     1261 gccaagggtg cgatccgcgc catgcaatgg gcgctggacg acgccgggct gacgcccaac
     1321 gacgtcgatt acatcaacgc ccacggcacc tcgacgccgg tcaacgatgc caccgagacg
     1381 ctggccatca agcggctctt cggcgagcgc gcctggcagg tgcccatcag cagcaccaag
     1441 tcggcaatgg gccacgccat gggcgcagcg ggaacgatcg aatcgatctt caccttattg
     1501 ggtcttggcc gggagatcat cccgcctacc tggaattatg agacgcccga tccggaatgc
     1561 gatctggact atgtgcccaa cgctccgcgg cccgcgccaa tccgcatcgc gctcaagaac
     1621 tccttcggtc tgggcggaca aaacgcctgt ctggtgctgg gacgctataa caacgaataa
     1681 aggagcggcc ggcggccggc gaccggcggc agatggtcgg ttattggtta ctgatgattg
     1741 accgctccgg gaactgatta ctaactatta tgatcattct acgcgacgac aaacgcattg
     1801 ctcgcctggg ccggttggcc cagattctca gttttgccgg tctggccgcc ctggttggtg
     1861 gcctgatcat cattttcgtg ttcgacaatc aggccaatgt ctttctttac cagttattgg
     1921 cgctgctggt gggcttctct ctatcgcagg tcgggcttca ttttgcccat cgctatctgc
     1981 gccagccgcg aatggatgaa gtgctggatc gggcggcgag caagttcgcc cgaaaggacg
     2041 gccgtctcta ccattatctg ctgcccgccc cccacgtgct gctgttgccg accggcgtat
     2101 tcgtgctgac ggccaagttt cagaacggcc gcatcacagc caatggcgac acctggcagc
     2161 agactgggtt gggcatgcgc cgcttctttg gccgcgagtc gctgggcaac cccacccgcg
     2221 aggccgaggc acaggtcgcc aggatggcgg agtatattca acaacacgcc cccgcggcgg
     2281 caggcgcgcc cctcttcccg atcatcgtct tcaccgctcc caacatcgcc tcgctggatg
     2341 tcaaagagtc gcgtatcccg gccgttcatg ctgccaaatt atcgggtgcg ctacgccagc
     2401 aatcgatcaa tctcaagcca ttgccgaagg cgcagtacga cgctctgcgt gccgccttcg
     2461 atggcacggc ggcgcacttg ctggagacga ccgtagaggg atcagaggag tagccctgct
     2521 cgctttggaa aacgcaaaga caggggaatt atcatggcga catccgtccg ggccttgctc
     2581 gacacagaac tgaatgaact caaccagtcg gttctgaccc tgagcagcat ggtcgatgaa
     2641 gccatcggcc gcgccatcga ggcgctgttg accaacgacg ccaccctggc cgggaacgtc
     2701 atcgccgggg acgcggagat caaccggctg agattccaca tcgaggaaaa agcctacatc
     2761 atcctgtcga cccagcagcc attggcccgc gatctgcgca cggtcatcgc cgctatccac
     2821 tttgccattg agctggagcg catcggcgat cacgccgcgg gtatcgcccg catcgtgcaa
     2881 cgtttgcaag agacgccaca accagtcgtc tttgaactcg ccgtgcccag cgaagtgacc
     2941 gagattgccg aaggaagtga ggtgggcgcg ccggtgcggc tgaaactgtc gcggctgccc
     3001 aagatgacca agcgtgcacg tgatatgctc catcgggcga ccgaagctta tattgaacgc
     3061 gacgtggaaa aagccaacgc catcgtgaaa cgtgaccgaa agatcgataa ccaatatcgg
     3121 aagttcttcg ccgaggcaat ggccgagatc gccaacaaac acgtgggtga aatccctacc
     3181 tatttactat ggatcgccca taatctggag cgcatcggcg accgtaccac aaacctggcc
     3241 gaacgcgtcg ttttcatggt gacgggccaa tataccgagg ttttagaaga ttacgggtaa
     3301 gtggggtttc aagttgacaa tcgacccatg tggctttaga attccaaccc catagcctgg
     3361 gttgctgggt atccccaaat ggggaaaaac caatgccgag gaggtggtgt tcaaaagggg
     3421 aatccaagta cctggcggcg atgtccgcac aaagaaatca ggtggtaacg accggcgatt
     3481 aaccggtctg gttttcaagg tcagacttga acattacgtt caaaatagaa tagaggagaa
     3541 gaatcatgaa acgtctgtca ctgcttttag tcttgctgct cctggcggca ctggcccttg
     3601 tggcctgcca gccggccggc ggcacaactg ctccagctga agaatcggcc ccggccgagg
     3661 aagccgcgcc ggccgaagaa gaagccgcgc cggctgaaga agaagctgcc gccgcgtttg
     3721 aatgcaccga cgccatcggt tgcgttgaag tcgccgccgg cgacccgatc cagatcgctt
     3781 cggcgctggt catttcgggc gcgaatgccg acctgggcct cgactcccag cgcggtgttg
     3841 aaatcgccct caagatgcgc cccgaagtgt tgggccacaa ggttgagttg atcgctgagg
     3901 atgatggttg tagcgctgag ggcggccaga cggccggcac caagatcgtc gccaacccgc
     3961 agatcgtcgg cgcgatcggc acgagctgct cgggcgccgg tgttcccttc gcccagatcc
     4021 tctccgatgc cggatacgcc acgatctcgc cgtcgaacac ctcccccgcg ctgaccgacc
     4081 ccaaccaggc gttcaagatt ggctacctgc gcaccgccca caatgacctc atccagggcg
     4141 cggccatggc cgactatgcc tatgacgtcc gtggcttcac caaggccgcc gccatccatg
     4201 acggcgaccc ctacaccgaa ggtctggcga aggcgtttgc cgacgctttc gtggccaagg
     4261 gtggcgagat ggtagccttc gaggcggaag ccgccgacgc gaccaacgtt gagccgctgc
     4321 tgacgacgat cgccgccgcc aagcccgaat tcctgtactt cccggtgttc atcccgttgg
     4381 gttcgctcat cgtcaacacc gccaagacga tccccggcct ggagtctgtc cagttggccg
     4441 ccgctgacgg cgtccagtcg cccagcttcc tggagaacgt cgtcggcacg ggtgagggta
     4501 tggttgtctc cggcccggac ctggccttca gcaacacctt ctataaggac gagttcctgc
     4561 cgatgtacga agccgactat ggcaccgccc cgacggctcc gttccatgcc cactccttcg
     4621 acgcgaccaa catgctgttg aacgcgatcg agaaggttgc ccagcaggac gccgacggta
     4681 acctgttgat cggccgccag gcgctgcgcg acgcgcttta cgacacttcc ggtatggaag
     4741 gcatcaccgg cacgctcacc tgcagcgagt tcggtgactg cgccgacccg aagatcgccg
     4801 tgagcgagat ccagagcggg gaatttgttc gcatttggcc ctaatctgaa tcggtaacct
     4861 aatcgggccg gcctctcttt gccgggaggc cggcccgttg ctctttccca aaaaccaaag
     4921 gctcgggttt gtgccggcga cttgcacccc gaagcctgtt gtgtataaaa ataatcgagc
     4981 cggttgaccc ggcgcgagca ggaggctttc aatgacatca tcacgacgcc tcaaagacct
     5041 ggactggatc ggccttgctc tatgggtctt ccgtgcagct atcatcattc ttgttgtagt
     5101 cggcacagtc cggaagctat tcttcggggt gggaacacaa tacacgggcc gggactggct
     5161 cgacttcgcg gtgtcgggtc tgtctcaggg gggattgtat gcgctcatcg cgcttggcta
     5221 caccatggtc tatggtgtgc tgttcatgat taacttcgcc catggcgaat tttttatgtc
     5281 cgg
//
LOCUS       DAOMQG010000237         5279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6587_length_5279_cov_11.877297, whole genome shotgun sequence.
ACCESSION   DAOMQG010000237 DAOMQG010000000
VERSION     DAOMQG010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6587_length_5279_cov_11.877297"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLC06_11970"
     CDS             complement(<1..245)
                     /locus_tag="PLC06_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRO91207.1"
                     /translation="MPSTRPNILLIVIDCLRADRAFDDDRTAQTPGMQALAERGTLFT
                     HLITANSMTIPCMTTMFSGMYPAHHGVRAMVGARVSD"
     gene            complement(432..1820)
                     /locus_tag="PLC06_11975"
     CDS             complement(432..1820)
                     /locus_tag="PLC06_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91208.1"
                     /translation="MARQEYLYQQTPAADTPGKSHSRADWVRQGWFLLLALVLLAELF
                     TPFLVWPLRLPRIILGVVEIALAMTVFATFIYMLKEDRVPRAVLLIIGLSLVWSLSPM
                     IEGQSLAATAWGWWGMFKYPFLALFAYLVQGWPPDFARRFFRFCIGLLIFQIIVQLIM
                     FALGFPIGDSLAGTFGLKGVMQFTMLVFFIVSLGLGHWLATYERKTLLLILALGLLGS
                     TLNATKFYLIGVGILAAATLLIHLIRGGQIRQLFIYIVLIAGAAAVFIPIYNSYLVNA
                     MGLKPLQEYLNLETAGNYIFTDGSGNGDLTYNIGRGLAITYGWQQIQRDLTTTLFGFG
                     IGTRSQSELLGIRGSVLQEDVYGVGVTTMGTWIQEIGVVGMLVFMTICLWMATKMFQY
                     ARRVVDPYRATLAYGLMLFTLFWPLWQWYHKAWTAGVMMTLYWVAIGYIFNQIYATGR
                     RRRVGRRIGRLS"
     gene            complement(1889..3034)
                     /locus_tag="PLC06_11980"
     CDS             complement(1889..3034)
                     /locus_tag="PLC06_11980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRO91209.1"
                     /translation="MEIPFVDLRAQYRQIQNEVDPAVLAVMQRGDFILGGAVTEFEKA
                     FAAYCGARYCIGVDSGYSALELIIRAYDIGPGDEVITAANTFIATTLGISNAGATPVL
                     VDCDPETYNIDVNKIEAAITPRTKAIMPVHLYGQPADMDVIRAIARKHNLYVFEDAAQ
                     ASGARYKGRMAGSLGDAAAFSFYPGKNLGAYGDGGAVTTNDADIAEKIRLLRNIGQKV
                     KYFHEIKGFNNRLDTMQAAVLGVKLPYLNDWNASRRRAAATYADLLVDLPIITPTTAD
                     YAEHIFHLYVVRVSDREALMEHLKKAGIATGLHYPIPIHLQPAYAELGYKRGDFPITE
                     AYAETIVSLPIFPELDDEKVAYVANAIHEYMAARGEAERLPEAVAAD"
     gene            complement(3130..4332)
                     /locus_tag="PLC06_11985"
     CDS             complement(3130..4332)
                     /locus_tag="PLC06_11985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO91210.1"
                     /translation="MKNEHRPSSDRLRVAWVSPVTPDKLDSATWVDTTRELRRQGADV
                     TLITIGPAGKQSYRGVEVLNIPRPKLYLLGQVLFHLGVLRYLLPRLREYDVILFHQLS
                     AIWLLPLRLAGRQRPRLVMDTRDMVDYTTDSFKIRLRTAWFNLVTDLAARFADGQTAI
                     TPRMAELVRIPKDQLWGIWPSAVEAERFAIAVKARHWPYQDEPVRLVYIGIFLAKRHL
                     LPLCCAVNRAGAEGMSFVLSLYGDGPLRPELERIAAESAGAVTVERPVPHENVPHILA
                     QSHVGVTSLPEVDDIKYEASSPIKLFEYMAAGMPVLATSNICHTDVVGDGQYAFWADD
                     VTEEALLVALRQIWADRAELSQLGQEAQTDVHGWTYVANAAKLKAALVYGLGRHEAAR
                     TSAEQVRA"
     gene            complement(4359..5009)
                     /locus_tag="PLC06_11990"
     CDS             complement(4359..5009)
                     /locus_tag="PLC06_11990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRO91211.1"
                     /translation="MLEVLDPILLEEPQSIDEGVTLGYPTGRSIADRTLRIGSEATIR
                     SGTVIYAGATIGRGLQTGHNVVIREENVIGDNVGIWSNTVIDYGCRIGNNVKIHNNVY
                     VAQFTTIEDDAFLAPGVSIANDMRPLCAECTHAGGPTIKRGARIGVNVTILPRVTIGE
                     YALVGAGSVVTRDVPPYAVVYGNPARVAMSVNDIDCPLYPDEKAYVDGLRHRCGAR"
     gene            complement(5002..>5279)
                     /locus_tag="PLC06_11995"
     CDS             complement(5002..>5279)
                     /locus_tag="PLC06_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91212.1"
                     /translation="FGEFSFAYHYGDVVIPYIRFEEPLRVQCQHFLECIREGKQPQTD
                     GLNGKRVVQVVEAAQRSMKNGGGTVYLNQNGHGDPAEARRAKELIHA"
BASE COUNT         1262 a         1515 c         1458 g         1044 t
ORIGIN      
        1 ctatcggaaa cacgcgcgcc aaccatggct cgcacaccat gatgggcggg atacatgccg
       61 ctgaacatgg tcgtcatgca cgggatcgtc atggaattgg cggtgatcag gtgcgtgaaa
      121 agcgtgcctc gctcggccag tgcctgcatg ccgggcgtct gggccgtgcg gtcgtcgtcg
      181 aaggccctat cggcacgcag acaatctatt acgatgagca agatgttggg ccttgttgaa
      241 ggcatttaac tatcctccgc gtattggagt tcggaaagcc ccggaaaccc ggcagccggg
      301 tattctccat tcattggtct ttatggatac catggtagcg cgttcgactt acaataaacc
      361 tgaagagaga aaagggggtg acagatagct ggttccgcct cgacatgcac cactaaggca
      421 gcaagcagcg ctcaactcaa tcgtccgatg cggcgaccaa ccctacgacg acggccggtg
      481 gcataaatct gattgaaaat ataaccaatc gccacccaat agagggtcat catgaccccg
      541 gcagtccagg ccttgtgata ccattgccag agcggccaga aaagggtgaa cagcatcagg
      601 ccataagcca acgtagcccg gtatggatcg acgacccgac gggcgtactg gaacatcttt
      661 gtggccatcc acaaacagat ggtcataaaa acaagcatgc ccaccacgcc tatttcctgg
      721 atccacgtcc ccattgttgt gaccccgacg ccatatacat cttcctggag cacacttccg
      781 cggatgccca gaagttcact ttgtgagcgc gtaccgatcc caaagccaaa caacgtcgtc
      841 gtcaggtcac gctggatctg ttgccacccg tacgtaatcg ccaggcctcg gccgatgtta
      901 tacgtgaggt cgccattccc cgacccatcg gtgaaaatat aattccctgc cgtctctaaa
      961 ttgaggtatt cctgcaacgg tttcagaccc atcgcattaa ccaggtagga attatagatg
     1021 ggaataaata cggccgcagc accggcaatt agcacaatat agataaacaa ttggcgaatc
     1081 tgaccgccac gaatgagatg aatgagaagt gtggcggccg ccagaatacc gacaccaatc
     1141 agataaaact tggtcgcgtt cagcgtgctg cctaataggc caagagcaag gatcaacaac
     1201 agagtcttcc gctcatacgt ggcgagccaa tggcccaggc cgagggagac gatgaaaaac
     1261 accagcattg tgaactgcat cacaccctta aggccaaacg tgccggccag gctatcgccg
     1321 attggaaacc ccagggcaaa catgatcagt tgaacaatga tctggaaaat cagaagtcct
     1381 atgcaaaacc ggaaaaagcg tcgggcgaag tcagggggcc agccctgaac aagataggca
     1441 aacagggcca gaaatggata tttgaacatc ccccaccaac cccaggctgt tgccgccaag
     1501 gattgcccct caatcattgg tgataagctc cagacaagcg acaggccaat gattaacagg
     1561 accgcgcgag gaactctgtc ctccttcaac atatagatga aggtggcaaa gaccgtcatg
     1621 gcgagagcta tctcaacgac acccagaatg atgcgcggca aacgcaatgg ccataccaaa
     1681 aagggcgtga acagctcggc cagcaagacc aatgccagca ggagaaacca tccctgccgg
     1741 acccaatcgg cgcgagaatg actctttccg ggggtatcgg ctgcgggtgt ctgctgataa
     1801 aggtattctt gtcgcgccat gttgaggttg tccggaagtc ggccattggt ttcaatagcc
     1861 gacttcaaac cggatgctaa cagataatct agtcggcggc cacggcctcc ggcaatctct
     1921 cagcctcgcc tctggcagcc atgtattcgt gaatagcgtt ggctacatag gcaaccttct
     1981 catcatccag ctcagggaag atgggcaacg agacgatcgt ttccgcatag gcttcggtga
     2041 tcgggaaatc gccacgcttg tagccaagct cagcgtaggc gggctgcaaa tgaatgggaa
     2101 ttggataatg cagccccgtg gcaatgccgg ctttcttcag gtgctccatc agcgcttccc
     2161 ggtcggaaac acggacgaca tagagatgga agatatgttc ggcgtaatcg gccgtggtcg
     2221 gggtgatgat cggtaaatcg accagcaagt cggcataagt cgccgcggcg cgccggcgac
     2281 tcgcgttcca atcgttcaga taaggcagct tgacgcccag cacagcggcc tgcatcgtat
     2341 caagtcggtt gttgaagccc ttgatctcgt ggaaatactt caccttctgg ccgatattgc
     2401 gcagcagacg aatcttctcg gcgatgtcgg cgtcgttggt cgtcaccgcg ccaccatcgc
     2461 cataagcgcc cagattcttg ccggggtaga agctgaaagc cgctgcgtcg cccaaactgc
     2521 cggccatgcg ccctttatag cgtgcgccgc tggcctgagc cgcatcctcg aagacgtaga
     2581 gattgtgttt gcgggcaatg gcgcgaatga cgtccatatc ggccggttgc ccatagagat
     2641 gaaccggcat aatcgccttc gtgcgcggtg tgatggcggc ttcgatcttg ttgacgtcaa
     2701 tgttatacgt ctcgggatcg caatcaacca gcaccggcgt cgccccggca ttggagatgc
     2761 ccagcgtcgt ggcgatgaac gtattggcag cggtgatgac ctcatcgccg ggaccgatgt
     2821 cataggcgcg aatgattaac tccagcgctg aataaccgga gtcaactccg atgcaatacc
     2881 tggcgccgca ataggctgca aaggctttct caaactcagt taccgcaccg cccaggataa
     2941 agtcgccacg ctgcatgaca gccagcacag ccgggtctac ttcattttga atctgacgat
     3001 attgtgctct caggtcgaca aatgggattt ccatgtgaat acctcaacgt tgtttaggat
     3061 ttggcgcgag caaaatctgc cgagccaact gtgttaatga cttcgagaaa ttaaaacgat
     3121 ctggcatatt taggcgcgaa cttgctccgc cgatgtgcgc gccgcctcat gccggcccag
     3181 gccatagacc agggcggctt taagcttggc ggcattggcg acatacgtcc aaccgtgaac
     3241 atccgtttga gcttcctgcc ccagttggga cagctcggcg cggtcggccc aaatctgccg
     3301 cagggccacc aacagggcct cttcagtcac atcatcggcc cagaaggcat attgcccgtc
     3361 gccgaccaca tcagtgtggc atatattact tgttgccaac acaggcattc cggcagccat
     3421 atattcaaac agcttaatcg gactggatgc ctcgtacttg atatcgtcga cttcgggcaa
     3481 ggaggtcacg cccacgtgag attgggccaa aatatggggg acattttcat ggggcacggg
     3541 gcgctcaacg gttacggcgc cagcgctctc tgccgcgatg cgctccagct ccggccgcaa
     3601 tggaccgtcg ccataaagac tcaggacgaa tgacatccct tcagcgccgg cacgattgac
     3661 cgcgcaacaa agcggcagca aatgacgttt ggccagaaaa ataccgatgt agaccaaccg
     3721 aacaggctcg tcctgataag gccaatgacg cgctttcacc gcaatggcaa accgctcagc
     3781 ctcgacggcg gatggccaga taccccacaa ctggtctttg ggaatcctga ccagctcagc
     3841 catgcgaggg gtaatggccg tctgaccatc agcaaagcgc gccgccaggt cggtcaccag
     3901 gttgaaccaa gccgtgcgca accggatttt aaagctgtcg gtggtgtaat ccaccatatc
     3961 gcgagtatcc atcaccagac gaggccgctg cctgccggcc agccgcaacg gcagaagcca
     4021 tatggctgac aactgatgga aaaggatgac atcatattcg cgcaaccggg gcagcaggta
     4081 gcgcagcacg cccagatgga ataacacctg gcccagcaaa tatagtttcg gtcgcgggat
     4141 attgagtact tcaacaccgc gataggactg cttccccgcc gggccaatcg taatcagggt
     4201 aacatctgcc ccctggcgac gtagctcgcg cgtggtatcg acccatgttg cggagtcgag
     4261 cttgtcgggc gtcaccggag acacccaggc cacacggagc cggtcgctgg acggtcgatg
     4321 ctcgttcttc atgctctccc ctgcaccagg agcaaggctc agcgcgcccc gcagcgatgt
     4381 ctcaacccat cgacatatgc tttttcatca ggataaagcg ggcaatcgat gtcgttgacc
     4441 gacatggcca cacgtgccgg gttgccatag acaacagcat agggtggcac gtccctggtg
     4501 acgacggagc ctgcgccaac cagcgcgtat tcgccgatgg tgacacgagg aagaatcgtg
     4561 acgttgacgc caatgcgagc gccccgtttg atcgtcggcc cgccggcatg ggtacattcg
     4621 gcgcagagcg gccgcatatc attggcaata gacacgccag gagccaaaaa tgcgtcgtcc
     4681 tcgatcgtcg tgaactgggc cacgtaaacg ttgttatgga tcttcacgtt gttgccaatt
     4741 cggcagccat agtcaatgac cgtgttgctc cagatgccca cgttatcgcc gatgacgttc
     4801 tcttcgcgaa taacgacatt atggcccgtc tggagcccgc ggccgatagt cgcgccggca
     4861 tagataaccg tgccggaacg aatcgtggct tcactgccga tacgcagcgt gcggtcggca
     4921 atggagcgcc ccgtgggata gcccagggtg acgccttcat cgatcgattg cggctcttca
     4981 agcaagatcg gatcgagcac ttcaagcatg gatgagttcc ttggcgcgtc gcgcttcggc
     5041 gggatcgccg tgaccgtttt ggtttaggta aacagtgccg ccgccgttct tcatggagcg
     5101 ctgggccgcc tcgacaacct ggacgactcg tttgccattg aggccgtcgg tctggggctg
     5161 cttgccctcg cggatgcact ccaggaagtg ctggcattgc acgcgcaatg gttcctcgaa
     5221 acgaatatag gggatcacca catcgccata atgataagca aagctgaact ccccgaagg
//
LOCUS       DAOMQG010000238         5242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6646_length_5242_cov_9.651629, whole genome shotgun sequence.
ACCESSION   DAOMQG010000238 DAOMQG010000000
VERSION     DAOMQG010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_5242_cov_9.651629"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..535)
                     /locus_tag="PLC06_12000"
     CDS             complement(104..535)
                     /locus_tag="PLC06_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91213.1"
                     /translation="MAKKLFDSGVFRSRPLFSATQVVLVLVIAGAIVFAIDYNNRVQA
                     GRLVGSGEEALQSQVDREATRQVELQVTLEYVSSDDYVAAYARNEGGMLLPGEHRIVP
                     MLQEATPVPTPSSPSTSDPVTQARPWQAWWRLLTDAPLPTR"
     gene            619..1491
                     /locus_tag="PLC06_12005"
     CDS             619..1491
                     /locus_tag="PLC06_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRO91214.1"
                     /translation="MESHAPTYPPIDFLSIGHICQDVVPGGFAIGGAAAYTAAVAATL
                     GCRAAIVTSASRQAEWSRELPGIAIHKSESRHSTIFENIYTPSGRVQIVRAVADDLSA
                     ADVPLSWTRAPMVYLGPIADEVDPALIHMFSNSIVGVGPQGWMRRWDEQGRVYQVEWE
                     STVEVLPLAAIAFLSTEDLARPALLEQYRRLAHVLVLTDGANGCTVYHQDEERVFLAP
                     VVPLVDTTGAGDIFAASYLVRFHQTGGDLWEAAEFANLIASRSVSFSGLRAKMEAIRD
                     LVMTGFGRPIERAE"
     gene            1507..2301
                     /locus_tag="PLC06_12010"
     CDS             1507..2301
                     /locus_tag="PLC06_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91215.1"
                     /translation="MARIYAIANQKGGVGKTTTVVNLGAYLAGSGRRVLVVDLDAQAN
                     ATSALGYDKNQMQISTYELLLEQLPLEDVRLHHEEFKLDLLPSSPSLAAAEIELVHVI
                     GREYRLRKALEKIDDEYDYVLIDSPPSLGLLTINALTAARDGVLIPVQCEYLPLEGLT
                     QLTQTIHKVQEYLNPSLQIRGVMMTMYDKRTNLSRQVVEEVRRHFPNKVFRTIIPRNI
                     SLSEAPSYGRPINHYSPQSPGAVAYKLLAMELLNGDRRNGGGDHAG"
     gene            2291..3148
                     /locus_tag="PLC06_12015"
     CDS             2291..3148
                     /locus_tag="PLC06_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRO91216.1"
                     /translation="MPAKRQALGRGLDALFSTSGERPARESGTMQLPIEQIIPNPRQP
                     RTVMDDQKLAELAASIKVHGLIQPLIVTETVEGYVLIAGERRWRASRLAGLDRVPVIV
                     KETTPQDMLELALIENVQRADLNALEEAHAYRQLIDEFGLTQEEVAARVGKARSTIAN
                     LVRLLALPESVQAAVNDGELSGAHARALLPLPAAEMQIAAMKQVLRLGLSVRQTEALV
                     ANLMAEKKPDPRQRKRLPPEMEELQGRFESALGTRVNIEKGPKGGRIVIYFYSDEDLN
                     SIYESIIDE"
     gene            3176..4378
                     /locus_tag="PLC06_12020"
     CDS             3176..4378
                     /locus_tag="PLC06_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRO91217.1"
                     /translation="MLERQLIQEKLGQATDILQELDIDVWLTFVRETSLSPDPALGLI
                     YDGDVTWQAAFLVGRDGRRIAVLGHYDAENVRTLGTYTEVIPYHEGILQPLLDTLATF
                     TPRTIAVNYSEDDVAADGLSFGMYRLLQGYLTGTPWEGKLISAEGIIRALRGRKTPTE
                     IELVRRAVATTERIFDEIEAFVRPGMTQKQIAEFVHGYIDGQGLGYAWPKPFNPIVTC
                     GPESATGHAAPGDVVLLPGHTFHIDLGVKQDGYCSDLQRMWYVLDEGETAAPSDVQRA
                     FDVVLGAIKVGESALRCGTPGWQVDEVAREFIVDHGYPEYMHAFGHLLGRSAHDGATV
                     LGPRWERYKGICDLQVEAGNIFTLELHVVVPERGIMSLEEDVLVTEQGVEYLSHPQTS
                     LRYIRPRG"
     gene            4472..>5242
                     /locus_tag="PLC06_12025"
     CDS             4472..>5242
                     /locus_tag="PLC06_12025"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRO91218.1"
                     /translation="MAGAFLGRVLGEWVGLPNGWVAAGLSLALGALGAWISGRRSIAR
                     TWPALIFLAYIAYPEPRAVYAALVAAVAAIAWALLLIDWPVPNRDRDSGVVAALLRPV
                     RPWAVAVGACFFLLYAFTLAPDILPADSGELQVVAAQLGVAHPPGFPLYTLAAHLFVK
                     LLPFVSPACAVNLFSAVTSAVTVSVVFLASWLITRDRIASLIAAVALGTATTFWSQAT
                     TANVRSLTGLFAALILFGLIAFRHEKMRAEQGRPDRWLI"
BASE COUNT          998 a         1451 c         1634 g         1159 t
ORIGIN      
        1 ggctacgagt tgatgaaatc cttgtgggtg acctgaggca ggccatcgat tggcatagat
       61 aaaacaaagg ccgacattcg agtcggcctt tgtcgttaag cgattagcgt gtcggaagtg
      121 gcgcgtcggt cagcaagcgc caccatgctt gccatgggcg cgcctgtgtt acggggtctg
      181 acgtagatgg ggacgaaggc gtcggtacgg gggtggcttc ctgcagcatg ggaacgattc
      241 ggtgttcgcc gggcagaagc attcccccct cgtttcgggc gtaggcggcc acataatcat
      301 cgctggacac atattccagt gtgacctgta attcgacctg gcgcgtggct tcgcgatcaa
      361 cctgggattg cagcgcttct tctccgctgc ccaccagccg gccggcctgg acgcggttgt
      421 tgtagtcgat ggcgaacacg attgcaccgg caatgaccaa caccagaacg acctgggtcg
      481 cgctgaatag ggggcgcgag cgaaacacac cggaatcgaa caacttcttg gccatcgagt
      541 taatgttaac tgcaatacgg gtcgaaggca acaggtaaag tgttgccgaa cggagagcgc
      601 gtaaggtaga atgcatcgat ggaaagtcac gcccccacat acccgccgat agattttctg
      661 tcgatcggcc atatctgtca ggatgtggtt cctgggggtt ttgctatcgg tggcgccgcg
      721 gcttatacgg cggccgtcgc ggcgacgttg ggctgtcggg ccgccattgt gaccagcgct
      781 tcgcggcagg ctgaatggtc acgtgaactg ccgggaattg ctatccataa gtctgaatct
      841 cgccactcca cgatcttcga gaatatctac acgccttccg gccgggtcca gattgtgcgt
      901 gccgtggcgg atgatctctc ggcggccgat gtgccgcttt cgtggaccag ggcgccgatg
      961 gtttaccttg ggccaattgc cgatgaggtt gatccggctc tcattcacat gttcagcaac
     1021 agcatcgtag gtgttggccc gcagggatgg atgcgacgct gggatgagca gggacgcgtg
     1081 tatcaggttg agtgggaatc gacggtagag gtattgccgt tggcggcaat tgcattcctg
     1141 agcacagaag acctggcccg cccggcgctc ctggagcaat atcggcgact ggcccatgtg
     1201 ttggttctca cagatggtgc caatggctgt acagtctacc atcaagatga ggagcgggtt
     1261 tttcttgcgc cggtcgtgcc tttggttgac accactggcg caggtgatat cttcgccgcg
     1321 tcgtatctgg tgcgcttcca tcagaccggt ggcgatcttt gggaagcggc cgagtttgcc
     1381 aatctgatcg cttcccgttc ggtgtcgttt agtgggctgc gggccaagat ggaggctatc
     1441 cgtgacctgg taatgacggg ctttggtcgg ccgattgaac gggccgagtg agttgaaagg
     1501 aaatccatgg ctcgaattta tgccatagcc aatcaaaagg gaggcgtcgg aaagacgacc
     1561 accgtcgtta acctgggcgc gtatctggca ggtagcgggc gacgcgttct ggtcgtcgat
     1621 cttgatgccc aggcaaacgc cacgtctgct ctgggatatg acaagaacca gatgcagata
     1681 tccacctatg agttgcttct ggagcaatta ccgcttgagg acgtgcgctt gcatcatgag
     1741 gagttcaaac tggacctgct gccttccagc ccttctctgg cggcggccga aatcgagctt
     1801 gttcatgtta ttggccggga gtatcgtttg cgaaaggcgc tggaaaaaat cgacgatgag
     1861 tatgactatg tattgatcga cagcccgccc agccttggcc tgctgacgat caatgccctg
     1921 acggccgcgc gcgatggtgt gctcatcccg gtgcaatgcg agtacctgcc cctggagggg
     1981 ttgacccagc tcacccagac tattcacaag gttcaggagt atttgaaccc ttcgttgcaa
     2041 attcgcgggg tgatgatgac gatgtacgac aagcgaacca accttagccg tcaggtcgtt
     2101 gaggaagtgc gccgccactt ccccaacaag gtgttccgca cgatcatccc acggaacatt
     2161 agcttgagcg aggcgcccag ctatggccgg cccatcaacc actactcgcc ccagtctccg
     2221 ggggcggtcg cctacaagtt actggctatg gaactgttga acggagaccg acgcaacggg
     2281 ggtggcgatc atgccggcta aacgacaggc tctcggccgc ggcctcgacg cgcttttctc
     2341 gacatccggc gagcgaccgg ctcgcgaatc gggaactatg caattgccca tcgagcagat
     2401 tatccccaac ccccggcaac ctcggacggt gatggacgac caaaagctgg ccgagctggc
     2461 cgcgtcgatt aaggttcacg ggttgattca accgctcatc gtcaccgaaa cggttgaggg
     2521 gtacgtgctc attgcggggg agcgccgttg gcgggcgtca cgcttggccg ggctggatcg
     2581 ggtgccggtc atcgtcaagg aaaccacgcc ccaggacatg ctggagttgg cgctcatcga
     2641 gaacgtccaa cgggccgacc tgaatgcgct ggaggaagcg catgcctacc ggcaactcat
     2701 cgatgagttt ggcctgacgc aggaagaagt agccgcgcgc gtcggcaaag cccgctcgac
     2761 gattgccaat ctggttcggc tgttggcgtt gcctgaaagc gtccaggcgg ccgtgaacga
     2821 cggcgaactg agcggcgccc acgcgagggc actcctgcca ttacccgcag ccgagatgca
     2881 gatagccgcc atgaagcagg tgctcaggct tggcttgagt gttcgccaga cagaggcgct
     2941 ggtggccaac ctgatggcgg aaaagaaacc cgatccgcgg caacgcaagc gcctgccgcc
     3001 cgaaatggaa gagctgcaag ggcgatttga atccgccctc ggaacgcgcg tcaatatcga
     3061 gaaagggcct aaaggcggcc gtatcgtcat ttacttctat tcagacgaag acctgaacag
     3121 catttatgaa tcgattatcg atgagtaata tttggatttc tcaggacgga ttctcatgct
     3181 agaacgccaa ttaatacagg aaaagcttgg tcaggcgaca gatattctgc aagagctgga
     3241 tatcgatgtc tggcttacct ttgtgcggga aacttcgttg tcgccggatc cggcgctggg
     3301 gttgatctat gatggtgatg tgacctggca agcggcattt cttgtcggcc gggacggccg
     3361 gcggatagct gttcttggcc actatgacgc cgaaaacgtg cgcacgctgg gcacctatac
     3421 ggaagtgatt ccgtaccacg aggggattct ccaaccgttg ctggatactt tggccacctt
     3481 tactcctcgc acaatcgccg tcaattactc tgaggacgat gtcgcggccg atggtttgag
     3541 ctttggcatg tatcgtctgt tacaaggcta tttgacaggg acgccgtggg aaggaaagct
     3601 catatcagcc gaaggcatca tacgcgctct gcgcggccgc aagacgccga ccgaaattga
     3661 gctggtacgc cgggcagtcg ccacgaccga gcgtatcttt gacgagatcg aggcgttcgt
     3721 gcgcccgggg atgacccaga agcaaatcgc tgaattcgtc catggctata tcgatgggca
     3781 ggggttgggc tatgcctggc cgaagccatt caatcccatc gtgacttgtg gccctgaatc
     3841 ggcgacgggc cacgccgctc ccggcgatgt agtgttgctc cccggccata cgttccacat
     3901 tgacttgggc gttaaacaag acggttattg ctccgatctg caacggatgt ggtatgtcct
     3961 ggatgaaggg gagacggccg cgccgtccga tgtgcagcgc gccttcgatg ttgtccttgg
     4021 agcgatcaaa gtcggggagt ctgctttgcg ctgcggcacg cccggctggc aggtggatga
     4081 ggtcgctcgc gaattcatcg tggatcatgg ctatcctgaa tacatgcatg ccttcggtca
     4141 tttgctcggc cgatcggctc acgatggggc aaccgtcctg gggccgcgct gggaacgcta
     4201 taaagggatt tgcgatcttc aggtggaagc gggcaatatc ttcacgctgg agctccatgt
     4261 cgtcgtgccc gaacgcggga taatgagcct cgaagaagac gtgctggtga cggagcaggg
     4321 cgttgagtat ttgagtcacc ctcaaacgag cttgagatat attcgccccc gcgggtgaat
     4381 cgacccgcat tgatcggagg ctccgctgac ctcgtcccgt tttgctgctc caacccgcat
     4441 cgtcaaactc ctgccctatg ccccgggtct ggtggcgggg gcctttctgg gccgcgtgct
     4501 gggcgagtgg gttggtctgc cgaatggctg ggtggctgcg ggactgtctc tcgcgctcgg
     4561 cgctctcggc gcatggatat caggtcgccg gtcaatcgca agaacatggc cggcgcttat
     4621 ttttctcgcc tatatcgctt accctgaacc tcgtgcggtt tatgccgcgc tggttgccgc
     4681 cgtggctgct attgcctggg ccttgttgtt gatcgactgg cccgtgccga accgcgatcg
     4741 ggacagtggg gtggttgctg ctctattacg ccctgtccgg ccttgggccg tggctgtggg
     4801 ggcgtgcttt ttccttttat acgcgttcac gcttgcgcct gatattctgc cggccgatag
     4861 cggagagctt caggttgtgg cggcgcaatt gggcgtcgcc cacccgccgg gcttcccgct
     4921 ctatacgctg gcggcccacc tgtttgtcaa gcttttaccc ttcgtttcgc ccgcctgcgc
     4981 cgtgaatctg ttttcggccg tgacgagcgc cgtcacggtt tcagtcgttt tccttgcctc
     5041 ctggctcatc acgcgggatc gtatcgcctc cctgattgcc gcggtggcgc ttggcactgc
     5101 gacgacattc tggtcgcaag cgactaccgc caacgtgcgc agtctcaccg ggttgttcgc
     5161 tgctctcatt cttttcgggc tgattgcctt tcgacatgag aagatgcgcg cggaacaggg
     5221 gcggccggat cgctggctca ta
//
LOCUS       DAOMQG010000239         5188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6726_length_5188_cov_13.016170, whole genome shotgun sequence.
ACCESSION   DAOMQG010000239 DAOMQG010000000
VERSION     DAOMQG010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6726_length_5188_cov_13.016170"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..298
                     /locus_tag="PLC06_12030"
     CDS             68..298
                     /locus_tag="PLC06_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002791634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91219.1"
                     /translation="MTAATIEIELDERTADVYTNASQDAREKLRVLLSIWLQEFEDET
                     SDLGQLMDEISENAVSRGLTPEILESLLLDEG"
     gene            288..710
                     /locus_tag="PLC06_12035"
     CDS             288..710
                     /locus_tag="PLC06_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRO91220.1"
                     /translation="MKGERRFVFDTNSVVSALLLKQSVSRQAFDRARRLGVLLVSLET
                     LSELSEVLHRAKFDRYVDEQERQRFLAGFVNEAELVEPSVTIRECRDPKDDKFLELAV
                     GGSAIMIVSGDRDLLVLSPFRNIIVLSPGEFLTYPIED"
     gene            808..1782
                     /locus_tag="PLC06_12040"
     CDS             808..1782
                     /locus_tag="PLC06_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO91221.1"
                     /translation="MIYVHFTNPLSADESSHLRPALSPDIILSEGPDLLAGTDILISG
                     RPTRAQLTAAPGLRALIIPWAGLPDEARDLMRDFPGITVHNLHHNAAPVAELAVALLL
                     AASKFILRYDRALRANDWRIRYERSTPTVLLEGRTALILGYGAIGRRVAVICRALGMT
                     TLATRRNLVAPTADGATEVHPAASLRELLPRAAAVIICLPHTPETDGLLGAAELALLP
                     TGAVLVNIGRGPIVDEKALYEALRDGHLSAAGLDVWYSYPPDAAARADWPPSACPFHE
                     LDNVVMSPHRGGAADETPRLRMAALAEMLNAAAEGREMGNVVDVGAGY"
     gene            1918..2235
                     /locus_tag="PLC06_12045"
     CDS             1918..2235
                     /locus_tag="PLC06_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91222.1"
                     /translation="MTMNTILNPDYEKTLINIVRDLPPNRVEQLVDFARFLEAQMLAE
                     ALFQEEDVTGIEADNARWDALLATDEGQTLLDKLAHEALAEHRAGQTKPMTFNNKGRI
                     TPG"
     gene            2232..2501
                     /locus_tag="PLC06_12050"
     CDS             2232..2501
                     /locus_tag="PLC06_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91223.1"
                     /translation="MKSITSSQFWKLYRALPEDVQRRADKSYALWRINPQAHGLYFKR
                     VGNQQAVYSVRIGQEYRALGLLEGDSVLWFWIGSHAEYERLLKHL"
     gene            2591..4891
                     /locus_tag="PLC06_12055"
     CDS             2591..4891
                     /locus_tag="PLC06_12055"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRO91224.1"
                     /translation="MRITTRFIAAILTTLLAGTVFTASGYQAPAERDPMQDLVISAEE
                     LTADIEKGTTIIGDELAPELSTGSSFTSQTIESPIPFNAVVPHWEGTNAEAISLQVRT
                     SADGETWDDWLAVGANHDWMVPGDTEVVGEMVLVPGEGVTHRFVQVQVLFNNMEEAGL
                     ETGLLPSDPASETSLSRLRLTFIDTSQGPSADELIDLQSELDRDETLLPENIDAYPKP
                     FVVSRDAWCRHERCNYTEGLEYYPVSHLIVHHTVSNNASTDWPAVVRAIWNFHTFSRG
                     WGDIGYNYLVDPNGVIYEGHNGGDNVIGTHASGANKGSMAVALLGTFTTYSPGIKPPQ
                     AMLNSAADLLAWKADQRDINVFDASRLPNLAWGLPHLMGHRDVYGTTECPGDQAHLLI
                     PWLRDQIATRIGFVNPYIYADEQSTAFTRSTTGSWLVPPYLCGFNNHAWYTWSVNNAS
                     QSTNWGEWRPNVPGDGRYRIDVYVPYCRTGRAETGSATYTITHAGGTTTRTVSQQANV
                     GLWITLGEYNLKKGNGNVIRLTDKTNDEGLGVWFDAIRLLPLSSPPPPPPPVPVNVDP
                     ASNAWKTSRTVAFTWRIDNPDTVTVTTLQVAADPSFANLVLVNQSWYGAPTTHTHTFA
                     ADFNRLYWRVLVSRANAPLISSVPTGFALDSTPPTSVVTSPLFYLPRVGQYVVSWLGT
                     DALAGVDRYHVDFRPAGGTWTRWLTDTRLSSVLFQPPTQNVVYEFRSVAVDRAGNIEA
                     AVDKADASTAGAIQLTRSTFYPIVGR"
     gene            4984..>5188
                     /locus_tag="PLC06_12060"
     CDS             4984..>5188
                     /locus_tag="PLC06_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRO91225.1"
                     /translation="MEQLPRLSNREWDVVNLLREGKSNKMIASALGITERTVEFHLKN
                     IYDKLQVGSRVELVLKLGESTVTE"
BASE COUNT         1078 a         1561 c         1510 g         1039 t
ORIGIN      
        1 atgagtaatc gttgaaggga ctgtaatggc ttgtatgtgc tataatgggt ttactgaagg
       61 tgagcgtatg actgcagcaa ccattgagat cgaattggat gagcggactg ccgatgtcta
      121 cactaatgcc tcgcaagatg cccgagagaa acttcgcgtg ttgttaagta tttggctaca
      181 agagttcgag gatgaaacat ctgatttggg ccaactcatg gacgaaatca gtgaaaacgc
      241 tgtctcgcgc ggcctaacgc cggaaatcct ggaatcccta ttactagatg aagggtgaac
      301 ggcggttcgt tttcgacacg aattcggtcg taagtgccct actgcttaag caatcagttt
      361 ctcggcaagc tttcgatcgt gcgcgaaggc tcggcgtatt gttggtttca ctcgagaccc
      421 tatcagagct atccgaggtc ctgcatcgag caaagtttga cagatatgtg gatgaacaag
      481 aacggcagag atttctggcc ggcttcgtga atgaagctga actggtcgag ccttctgtca
      541 ccattcgcga atgtcgcgat ccgaaggatg acaagtttct cgaattagct gtcggcggct
      601 cagcgataat gattgtcagc ggtgaccgcg atcttctcgt cttgagtcca ttccgaaata
      661 ttattgtttt gtcgcccggc gaatttttga cctatcctat tgaggattga tttcagcccg
      721 tcaatcggca ggttgcctct tcacagaaga agtattgatg acgaaaaagg tggattaatc
      781 gacctcagcc ataccgtcga agccggcatg atctacgtcc acttcaccaa ccccctatcg
      841 gccgatgaat ctagtcatct aagaccagcc ctctcgcccg acatcatcct gagcgagggg
      901 ccggatttgc tcgccgggac cgacatcctc atctccggcc gccccacccg cgcccaactg
      961 acggccgcgc cgggcctgcg cgcgctcatc attccctggg ccgggctgcc cgacgaggcc
     1021 cgcgacctca tgcgcgactt ccccggcatc accgtccaca acctgcacca taacgccgcg
     1081 cccgtggccg agctggccgt ggcgctgctg ctggccgcgt ccaagttcat cctgcgctac
     1141 gaccgggcgc tgcgggccaa cgactggcgc atccgctacg agcgctccac gccgacagtg
     1201 ctgctggagg gccggacggc gctcatcctc ggctatgggg ccatcggccg ccgggtggcc
     1261 gtcatctgcc gcgcgctggg catgacgacg ctggccacgc gccgcaacct cgtcgcgccg
     1321 acggccgacg gcgcgaccga ggtccatccg gccgcttcgc tgcgcgagct gctgccccgc
     1381 gccgcggcgg tcatcatctg cctgccccac acgccggaga cggacgggct gttgggcgcg
     1441 gccgagttgg ccctcctgcc caccggcgcg gtgctggtca atatcggccg cggccccatc
     1501 gtggacgaga aggcgctcta cgaagccctg cgcgacggcc acctgagcgc cgccgggctg
     1561 gacgtgtggt atagctaccc gcccgacgcg gccgcccgcg ccgactggcc gccgtcggcc
     1621 tgtccgttcc acgaactgga taacgtcgtc atgagtcccc accgcggcgg cgcggccgac
     1681 gagacccccc ggctgcgcat ggcggcgctg gcggagatgc tgaacgcggc cgccgagggt
     1741 agggagatgg ggaatgtggt ggatgtgggg gcggggtatt aggggttgtc gtaatttgta
     1801 aagccggcca aaaacgaacc ccacgccgtt aaaccacttg ccgggtttct ggtacaattg
     1861 cagacacaat aggccgaaag agaaaaagct ggaagcgaat taacaaaggg tgctgctatg
     1921 accatgaata cgatcctcaa tccggattac gaaaaaacac taatcaacat cgttcgcgac
     1981 ttgccaccca atcgagtcga gcagctggtg gattttgctc gcttcctgga agcccagatg
     2041 ttagcggaag cactcttcca ggaagaggac gtgaccggga ttgaagccga caacgcgcgg
     2101 tgggatgcat tgctggcgac ggatgaagga caaaccttgt tggataaact ggcccatgag
     2161 gcgttggctg aacaccgggc aggccaaaca aagccaatga ccttcaacaa taaagggcgc
     2221 atcacgccgg gatgaaatca atcacatctt cacaattctg gaagctttac agggcattac
     2281 cggaagacgt gcagcgtaga gcggataaat cgtatgcgtt atggcggata aatccacaag
     2341 ctcatggcct gtattttaaa cgggtgggaa atcagcaggc tgtttattca gtccgaattg
     2401 ggcaggagta tcgagcgctt ggcttgcttg aaggtgattc tgttttgtgg ttttggattg
     2461 gatctcatgc tgaatatgaa cgattattga agcacctttg aaatccaccc tccgttgcct
     2521 ccctcccctc ccatctgcta gaatgtcaca tgaggtagcg ggcagttagt aatcgtcccg
     2581 ctctgcgccc atgagaataa cgacgcgatt catcgccgcc atcctgacga cactcctggc
     2641 cggaaccgtc ttcaccgctt ccggctatca ggcccccgcc gaacgcgacc ccatgcaaga
     2701 cctcgtcatt tccgccgagg agttgacggc cgatatcgag aagggcacga cgatcatcgg
     2761 cgatgagctg gcacccgaac tgtctaccgg ttcatccttc acctcgcaga ccatcgaatc
     2821 ccccatcccg ttcaatgcgg ttgtacccca ttgggagggc accaacgccg aggccatctc
     2881 gctgcaggtg cgcaccagcg ctgacggcga gacatgggac gactggctgg cggtcggcgc
     2941 caaccacgac tggatggtgc cgggcgatac cgaggtcgtc ggcgagatgg tgcttgtgcc
     3001 cggtgagggc gtcacccatc gcttcgtgca ggtgcaggta ctgtttaata atatggaaga
     3061 ggccgggttg gaaaccgggt tgctgccgag tgatccagct tccgagacca gcctcagccg
     3121 gttgcgcctg accttcatcg acaccagcca ggggccgagc gccgacgagt tgatcgatct
     3181 gcaatcggaa ctggaccggg acgagacgct gctgccggag aacatcgacg cctaccccaa
     3241 gccgtttgtc gtcagccgcg atgcgtggtg tcgccacgag cggtgcaact acaccgaagg
     3301 gctggagtat tatccggtca gccatttgat cgtccaccat accgtttcga ataatgccag
     3361 caccgactgg ccggccgtcg tgcgggccat ctggaacttc cacactttca gccgcggttg
     3421 gggggacatc ggctataact atcttgtcga tcccaacggc gtcatctatg agggccacaa
     3481 cggtggcgat aacgtcatcg ggactcacgc atcaggggct aacaagggca gcatggccgt
     3541 ggccttgctg ggcaccttca ccacctattc gcccggtatc aagccgccac aggccatgct
     3601 caactcggcc gccgatttgt tggcctggaa ggcggatcag cgcgatatca acgtctttga
     3661 cgcgagccgg ctgcccaacc tggcctgggg gctgccgcat ctgatgggcc accgtgacgt
     3721 ctacggcacg accgaatgcc ccggcgatca ggctcatttg ctgattccct ggctgcgtga
     3781 tcagatcgcg accaggatcg gcttcgtcaa tccctatatc tatgccgatg agcagtcgac
     3841 ggccttcacg cgcagcacga ccggcagctg gcttgtgccg ccctatctgt gcgggttcaa
     3901 caaccatgcc tggtatacct ggtcggtcaa taacgcctcg caaagcacga actggggtga
     3961 atggcggccg aatgtgcccg gcgacggccg ctaccgcatc gatgtctacg tgccctattg
     4021 ccgcaccggt cgcgctgaaa cgggcagcgc cacctatact atcacccacg ccgggggcac
     4081 gaccacgcgc accgtcagcc agcaggctaa cgtcggccta tggatcactc tgggcgaata
     4141 caatttgaag aagggtaacg ggaacgtcat tcgcctgacc gataagacca atgacgaagg
     4201 gttgggggtc tggttcgacg ccatccggct gctgccgttg agttcgccgc ctccgccgcc
     4261 cccgcctgtg ccggtaaatg tggatccggc gagcaacgcc tggaagacca gccgcacggt
     4321 tgccttcacc tggcggatcg acaaccccga cacggtgacg gtcactacct tgcaggtggc
     4381 ggccgatcct tcgttcgcca acctagtgct ggtcaaccaa agctggtacg gcgcgccgac
     4441 gacccacacc cataccttcg ctgccgactt caaccggctc tattggcggg tgctggtgtc
     4501 gcgagccaac gcgccgctca tctccagcgt gccaaccggc ttcgcacttg attccacacc
     4561 cccgacatca gtcgttacct cgccgttatt ctacttgcct cgcgttgggc agtacgtggt
     4621 gtcctggttg ggcacggacg cgctggccgg cgtcgaccgg tatcatgtcg attttcggcc
     4681 ggccggcggc acgtggaccc gctggctgac cgatacccga ctgtcgtcgg tcctcttcca
     4741 gcccccgaca caaaacgtgg tttatgagtt ccgctccgtg gccgtggaca gagcagggaa
     4801 tatcgaggcg gccgtcgaca aggccgatgc ctcaaccgcg ggggccatcc aactgacccg
     4861 gagtactttc tatccgatcg tggggcggta ggcatggtca gacccgattg gcagacgaca
     4921 tagaagcccg tttctcaata gataggatag ttcacctgtt cgtcattgaa tttacataac
     4981 actatggaac agctaccccg attgagcaac cgtgaatggg atgtggtcaa tcttctccgt
     5041 gagggaaaaa gcaacaaaat gattgcgtcg gccctcggca tcaccgaacg caccgtcgaa
     5101 tttcatctaa aaaatattta tgacaagcta caggtcggtt cacgggtcga actggtgctg
     5161 aaactggggg aatccacagt tacggaaa
//
LOCUS       DAOMQG010000240         5174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6747_length_5174_cov_9.032624, whole genome shotgun sequence.
ACCESSION   DAOMQG010000240 DAOMQG010000000
VERSION     DAOMQG010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6747_length_5174_cov_9.032624"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLC06_12065"
     CDS             <1..858
                     /locus_tag="PLC06_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRO91226.1"
                     /translation="PLSEAGTIGFEYGYSIQAPERLVVWEAQYGDFINTAQAIIDEYV
                     TSGNAKWELKSSLVLLLPHGYEGQGPDHSTGRLERFLQLAAETNIRVANATTAAQYYH
                     LLRRQAALLMTDPLPLIVMTPKSLLRNPKVYSSLRDLAEGQWQPVIDDVLAAANRTAV
                     RQIIMCSGKIYFDVVSDPRRAERTDIALVRVEQLYPLETDAISRIIESYPNAAEVVWL
                     QEEPANGGAWDSMHQRLQHILNGSLPLRLIARPRRASPAEGSMGMHALHQATLIDRAY
                     DLPQEVTNS"
     gene            855..2090
                     /gene="odhB"
                     /locus_tag="PLC06_12070"
     CDS             855..2090
                     /gene="odhB"
                     /locus_tag="PLC06_12070"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRO91227.1"
                     /translation="MSTEIVVPELGESVVEATVGEWRVKVGDSVKIGDIVVELETDKV
                     DVEVGATAAGVIADILQPAGSDVKIGDVLGNIDANGTAVVPGKAAPKAEEIATVPEKS
                     AENDRATPVARRMAEAEGIDLSQVSGSGTGGRVTKQDITARVEPKQALAAETPAPRPE
                     VAPRPMPSPTPQPVPSMTAGREERVRMSRRRRTIAQRLVEAQQTAAMLTTFNEVDMGA
                     VMDLRSRRQEAFVKRHGVKLGFTSFFVKAVIGGLKAFPRLNAEIQDDEIVLKHYYDIG
                     IAVGAEEGLVVPVIRDADRKSFAQIEAEVRDFAEKSRNGTLSLEDLRGGTFTITNGGV
                     FGSMLSTPILNPPQVGILGLHNIQERPVVRDGEIVIRSMMYLALSYDHRIVDGREAVQ
                     FLVRVKQLIEEPEALLLEG"
     gene            complement(2141..3406)
                     /locus_tag="PLC06_12075"
     CDS             complement(2141..3406)
                     /locus_tag="PLC06_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRO91228.1"
                     /translation="MHMSNDTSIKRRILFLTTIRSYRLEAFRVAAEKLGVEVTTGLDL
                     PEALTEQWPDALPLNFADIGEAARKIAEYAQHNPLSAILSVDDSGSLVAAAASNLLGL
                     PHNDPAAAVAARDKAVMRRMLGAGGVAVPWFREYTTADDPAVVSAEAPYPCVVKPVNL
                     NGSRGVMRADSPSRLISAIERLTRLITTDNPDPRPYLVEEYLPGVEVALEGMLNRGAL
                     TVLALFDKPDPLEGPFFEETIYVTPSRLPVGTQTAIEATTTAAARALGLVSGPIHAEL
                     RINEGGVWIIEIAGRSIGGLCSQVLRFGVDASLEELIVRQAAGLSLGDTTRSDRAAGV
                     MMIPIPEAGLLREIIGVDEAAAIPLIESVEITARLNYPLVPLPEGDSYLGFIFARGET
                     PEAVESALRQAHERLKFRIDALLPIFSLQ"
     gene            3733..4992
                     /locus_tag="PLC06_12080"
     CDS             3733..4992
                     /locus_tag="PLC06_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91229.1"
                     /translation="MATTDLPAKASAAGDKLDAIVATSNTATPAGYIAPIMGSPHDPT
                     PSPVPVPTLGAPLPPPTVVVALPPIDFEAARADAQSKGLDIAFARVGFHTGPGGNATG
                     LGEWMRDLNAAGVPFFLKSADAAGPIHEAQLLMKENEAAGRFVDHTLVFRLTEPRYEA
                     PFYDTSLSPEEAAAVSWQLNRDNKPPELEKEYIWLETLNEPGREGSDGQLQIERLGRF
                     SLETAKLAVADGYRYGAFAFSTGVPEPEDWEHPAMLDFLRYAGEHPDKVAVALHEYSL
                     TTQYIGAWYPYLVGRFQALFEVCDKYGIPRPTVLITEWGWEYADVPEPVPGVEDMAWA
                     SWLYAAYPQVKGAAVWYLGPQFGGIAERAHLLILPMRDYAVSHYFIIDPGIGRIDAEL
                     FRPNPPTLLGIEPLGLPTPFPRPQTMP"
     gene            complement(5059..>5174)
                     /locus_tag="PLC06_12085"
     CDS             complement(5059..>5174)
                     /locus_tag="PLC06_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO91230.1"
                     /translation="YSSEFEVERMYRDTRLMTIGEGTSEIQRLVIARHILQ"
BASE COUNT         1085 a         1639 c         1500 g          950 t
ORIGIN      
        1 ccgctctcgg aggcaggcac catcggcttc gaatatggct acagcatcca ggccccggag
       61 cggctggtcg tgtgggaggc gcagtacggc gacttcatca acacggccca agccatcatt
      121 gacgagtatg taacctccgg caatgccaaa tgggagctga agtcgtcgct cgtcctgctg
      181 ctgcctcatg gttatgaagg gcaaggcccc gaccattcaa ccggccggct ggagcgattc
      241 ctgcaactgg cggccgagac caacattcgc gtcgccaatg ccacaaccgc ggcacaatac
      301 taccatttgc tacgccggca ggcggccctg ttgatgaccg atccgctgcc gctcatcgtg
      361 atgacgccca agagccttct gcgcaacccg aaagtctatt cctccctgcg cgatttggcc
      421 gagggccaat ggcagccggt catcgacgat gtgctggctg cagctaaccg caccgccgtg
      481 cgtcaaatca tcatgtgcag cggcaaaatc tacttcgacg tggtcagcga tcctcgccgc
      541 gccgaacgca ccgatatcgc tctggttcgc gttgaacaac tctatccgct cgaaacggac
      601 gcgatttccc ggataatcga atcttatccc aacgcggccg aggtcgtctg gctccaggaa
      661 gagccggcca acggcggcgc ctgggattca atgcaccagc gactgcaaca cattcttaat
      721 ggctccctgc cgttgcgcct catcgcccga ccacgccgcg ccagtccggc agaaggctca
      781 atgggcatgc atgctttaca tcaggcgact ctgatcgaca gagcctacga tctaccccaa
      841 gaggtgacca actcatgagt acggaaattg tcgtgcccga actgggcgaa tctgtcgttg
      901 aggcgactgt cggcgagtgg cgagtgaaag tcggcgactc ggtcaaaatc ggcgatatcg
      961 tcgtcgagct tgagaccgat aaggtcgacg ttgaagttgg ggcaacggcc gcgggcgtga
     1021 tcgccgatat ccttcaaccg gcgggcagcg atgtcaagat cggcgatgta ctgggcaata
     1081 ttgacgccaa cggcaccgcc gtcgtaccgg gaaaggccgc gccaaaggct gaagagatcg
     1141 ccacagtccc tgagaaaagt gccgagaacg atagagcaac gccggttgcc cgccgcatgg
     1201 ccgaggccga aggcatcgat ctatcacaag tatcgggcag cggcacgggt ggtcgagtca
     1261 ccaagcagga catcaccgcg cgcgttgagc caaagcaggc cttggcggcc gaaacaccgg
     1321 ctccccgacc cgaagtcgcg cctcgtccca tgccgtctcc gacgccgcaa cccgttccgt
     1381 ccatgacggc aggtcgcgag gaacgcgtgc gcatgtctcg ccgccgccga accatcgccc
     1441 agaggctggt cgaggcccaa caaacggcgg ccatgctgac caccttcaac gaggttgaca
     1501 tgggcgcggt gatggacttg cgctcgcggc gacaggaagc gttcgtcaag cgccatggcg
     1561 tcaagctcgg cttcacctct ttcttcgtca aggcagtaat tggcgggctg aaagccttcc
     1621 cgcggctcaa cgccgagatt caggatgacg agatcgtcct caaacattat tacgacatcg
     1681 gcatcgccgt cggcgcggaa gaagggctgg tcgtccccgt gatccgcgac gccgatcgca
     1741 aatcttttgc acaaatcgaa gctgaggtgc gcgacttcgc cgagaaatcc cgcaacggca
     1801 cgctgtcgct ggaggatttg cgcggcggca cgttcactat caccaacggc ggcgtcttcg
     1861 gctcaatgct gagcacgcca atcctgaatc cgccccaggt gggtatcctc ggcctgcaca
     1921 acatccagga gcggccggtc gttcgcgacg gggaaatcgt catccgttcg atgatgtatc
     1981 tggctctcag ctacgaccat cgcatcgtcg acggccgcga agccgtccaa ttcctggtgc
     2041 gggtgaaaca actcatcgag gagccggagg cgttgttact ggaaggataa attgcggata
     2101 gcattcaatg atcagcaacc tgtttgaagc atgaggctga ttactgaagg ctgaagatcg
     2161 gcaggagggc atcaatcctg aacttgagtc gctcgtgagc ctggcgcagg gctgactcaa
     2221 cagcctccgg cgtctctccc cgagcgaaga taaaccccag atagctatcg ccctccggaa
     2281 gcgggacaag cggataattg aggcgagcgg tgatctccac gctctcgatg agtggaatgg
     2341 ccgccgcctc atccacgccg attatctctc gcaacaaccc cgcctccgga atggggatca
     2401 tcatcactcc cgcggcccga tcactacggg tcgtatcgcc cagcgacaag ccggccgcct
     2461 ggcgcacgat cagctcttcg agcgacgcat ccaccccaaa gcgcagcacc tgcgagcata
     2521 atccaccgat ggagcgcccg gcaatctcga taatccacac gccgccctca ttgatgcgca
     2581 gttcggcatg gatcgggcct gataccagcc ccagcgcccg ggccgcggcg gtcgtggttg
     2641 cctcgattgc cgtctgtgtc cccaccggca atcgggacgg cgtcacataa atggtctcct
     2701 cgaaaaacgg cccttccagc gggtcgggct tatcaaagag cgccagcacg gtcagcgcgc
     2761 ccctgttcag cataccttca agcgccacct cgacgccggg caggtattcc tccaccagat
     2821 atggccgcgg atcgggatta tcggtcgtga tgaggcgtgt caatcgctca atagctgaaa
     2881 taagccggga tggactatcg gcgcgcatga cgcctcggct accgttgaga ttgaccggct
     2941 tgacgacgca aggatagggc gcttccgcag agacgaccgc cggatcatcg gccgtggtat
     3001 attcacggaa ccacggcacg gcaacaccac ccgcgcccaa catccggcgc atgaccgcct
     3061 tgtcgcgagc agccacggcc gcggcggggt cgttatgcgg caagccaagc agattactgg
     3121 ccgcggcggc caccaggctg ccgctgtcat cgacggacag aatcgccgag agcgggttgt
     3181 gttgagcgta ctccgcaatc tttcgcgcag cttcgcctat gtcggcaaag ttcaacggca
     3241 gcgcgtccgg ccattgctcc gtgagtgctt cgggcagatc aagtccggtc gtcacctcga
     3301 cacccagctt ctcggcggcg acgcgaaatg cttccaaacg ataggaacgg atggtcgtga
     3361 ggaagagaat ccgtcgcttt atcgatgtat cattgctcat atgcatgatt atactccagg
     3421 gacggcatgg aatccgtgtc gcagcaactc aattttttta cgatctccgg caccaaataa
     3481 acgatattgc gttatagtag gagcagacaa atcaatcctg acgagaggtc gaccgtctac
     3541 tttggccggc cccgaacata caggctcagt ggcttcgcgg tatacaagca gtagggagag
     3601 actcatttgt cgaaagaaac caatgaccaa catgccggca gtctttcgcg actgaagaga
     3661 gccaacccgt ccgtcttcat gggcgtcctg ctcgcgagta tccttgtggc tgccggatgc
     3721 gcgtctctgt caatggcaac aaccgacttg ccggcaaagg catccgccgc cggcgacaaa
     3781 ctcgatgcta tcgtcgcgac atccaacacg gcgaccccgg ccggttatat cgctcccatc
     3841 atgggcagcc cccatgatcc gacgcccagc cctgtccccg tgccgaccct gggcgcacca
     3901 cttcccccgc ccacggtggt cgtggcgctg ccgccgatcg acttcgaagc cgcgcgcgca
     3961 gatgcacaat ccaaaggact tgacatcgcc ttcgccagag tgggcttcca taccggcccg
     4021 ggcggcaacg ccaccggtct gggtgaatgg atgagggatc tcaacgcggc cggcgtccct
     4081 ttcttcctca agagcgctga cgccgccggg ccaatccatg aggcgcagct gttaatgaaa
     4141 gaaaatgagg ccgccggccg gttcgtcgat cacaccttgg tcttccgact cacagagccg
     4201 cgctacgagg cgcccttcta cgacacatct ctatcgccgg aggaagccgc ggccgttagc
     4261 tggcaactca accgcgacaa caaaccgccc gagttggaaa aggaatatat ctggctggaa
     4321 acactcaacg aaccgggccg cgaaggcagc gacggccagt tgcagatcga gcggctgggg
     4381 cgcttctcgc tggagacggc caagctcgcc gtagctgacg ggtatcgtta tggggcattt
     4441 gccttttcca ccggcgtgcc ggagcctgaa gactgggagc atccggccat gctcgatttt
     4501 ctgcgatacg cgggcgaaca ccccgataag gtcgcggtcg cccttcatga gtacagtctg
     4561 accacccaat atatcggggc gtggtacccc tatctggtcg gccgattcca ggcgctattt
     4621 gaggtctgcg ataaatatgg catcccgcgg ccaaccgttc tgatcacaga atggggttgg
     4681 gaatatgccg acgtaccgga gccggttccc ggcgtcgagg atatggcctg ggcctcgtgg
     4741 ctttatgccg cctatccgca agtaaaaggg gcggccgtgt ggtatctagg cccgcaattc
     4801 ggcggcatcg cggagagagc gcacctgctc atcctgccca tgcgcgatta cgccgtaagc
     4861 cactatttca tcatcgaccc gggcatcggc cggatcgacg ccgaactatt ccggcccaat
     4921 ccgccgacgt tgctggggat cgaaccgctt ggcctgccga cgcctttccc gcggccgcag
     4981 accatgccct aagtcaaacg aaaaggggcg caatgttgat cgcgcccctt gataacccga
     5041 cctagctgtc cgtcttgctc actggagaat atgacgggcg atgaccaatc gctggatttc
     5101 gctcgtacct tcgccgattg tcatcagtcg cgtgtcgcga tacatgcgct ccacctcgaa
     5161 ctcggaactg tagc
//
LOCUS       DAOMQG010000241         5157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6782_length_5157_cov_7.539200, whole genome shotgun sequence.
ACCESSION   DAOMQG010000241 DAOMQG010000000
VERSION     DAOMQG010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6782_length_5157_cov_7.539200"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..2252
                     /locus_tag="PLC06_12090"
     CDS             285..2252
                     /locus_tag="PLC06_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRO91231.1"
                     /translation="MNTAQKQTGWGFAARWVAIITVTFLIGTAFAFASMWSFGGPILE
                     RLDARIAGPVSGVWVGALMGLGLGVGQAIALRGRGISPTRWVASSTLGSAAGFALFVA
                     LTAQGGTQRTQLALLAGGMAGLGIGVAQWMQLRGRLSNAIIWVPVTVVAFLPVALLAY
                     NGSEVNAGLVMLAMGLAVACITGLGAMWLFGKGSPAIAAQVSVLLLFGLSLAACGSSS
                     QSDIPLPPTISPDAHRIFHNGVVLTMNPDRPSAEAISLAGDRIEAVGSDADVLALRRP
                     NSVVVDLQGRTLMPGFVDPHTHIFNDAEQYLDMSVDEVQQVALENGITTIGDMYVTED
                     FLGEMQEMERAGQLQIRTSLYLVMTDPCGESQGDWWQEYPPTRTPGEQLRIGGVKIFT
                     DGGVCKNPGLSYELEPGGGLGDLFQAQEELNGLVADAQARGFQVVMHACGDRAIDQAQ
                     NAIAAALGGQPNTFRHRIDHNCVIRPEQLARYGEIGIVPAVFGPYALCEPFGPPPPPE
                     YQAWEWPWRALLDANPGLPVAWHGDDPWFGRIRPLDDLYSLVTRNEVGEDGRVCEAPD
                     WQKAHTVTVAEALPMMTINAAYALFRDEEVGSLEPGKYADLIVVTGNPMAIEPEAIRE
                     LNVALTMIGGRAVYCADDSQNLCPTSETPTE"
     gene            2305..4413
                     /locus_tag="PLC06_12095"
     CDS             2305..4413
                     /locus_tag="PLC06_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRO91232.1"
                     /translation="MAHAQEQPAITSPIHSSRWRIWLARAGWLLIFALITGIFIASAP
                     SARYNELSGWQFGYAAQAIRPYIHVYTFAAIVVACRLAVVAIFYAVALLIAFRKWGDW
                     FALFVSAALLLVVWGFGARSDTSSYVFPIWLSEVEPLLTGLLNLLFVASWVLLFYLLP
                     DGRFVPRRAGWLTLLIMPGLLLSTLSSYSAIPYQYRIRIAEFAWGILFIGILITILVG
                     LYFQLYRYRNVATPAQRQQMKWILVGLAAQPLPLLFGITGLGGSWEAMLAIFVPLFVV
                     TLLPVTIGLAVLRRRLWDVDPLINRSLVYGGLTLLVLVIYIALVGLAFVLFGERSNLL
                     LAALAVGVAALLAQPIRARLQQAVNRLMYGDRDDPITVISELGRQLEHAAAPGDSLAT
                     LVETIGRSLKLPYVAIVRQENDNHDLVAEVGTSQPDLLRLPLIYQSQQVGELLVAPRA
                     PGESFTAAERYLLDNVARQAGAAVYSAQLTDHLQRSREQLVLAREEERRRLQRDLHDG
                     LGPQLATLSMQLDVARRLAAEGSPEAAELLGRLSGETQAAIADIRRLVYGLRPPALDQ
                     LGLAGALREYVAGQNQGTSGTRVTVEAPDALPPLPAAVEVAAYRIAQEAVANCLRHAH
                     ATECLINLNVREELSLEIRDNGRGLPSAVGGRESAFGVGLVSMRERAAELGGVCRIES
                     APGGGTRVMAIFPLLQEPQP"
     gene            4410..5069
                     /locus_tag="PLC06_12100"
     CDS             4410..5069
                     /locus_tag="PLC06_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91233.1"
                     /translation="MTETISVFIADDHRLFRDGLRALLAPLADMAVVGEAANGEESVA
                     LVEQLQPDVVLMDIQMPALNGIDATRRITAASPHMGVIIVTMFEDDDSVFAAMRAGAR
                     GYILKGAGQEEVTRTIRAVARGEALFGPAIAARLMSYFSDPRPSAPAELFPELTVRER
                     EVLNLIAAGHNNEAIAARLVVSRKTVRNHVSNIFSKLQVVGRAEAIIRARDAGMGDAN
                     S"
BASE COUNT          889 a         1646 c         1627 g          995 t
ORIGIN      
        1 acgggctgta ccagcggtga tattcacgat gcattggtta tgattccctc tcctgaacga
       61 cgatttggtg gacgccctgc ccgatgacca atcaggttag cgggatatga tagcacatga
      121 ttggggtgca agcatccaat acaaagtggg gcaattacga ggaatatctt ctgccggcgg
      181 gaaccgtcag gcatatgatt cccgcatcac acaggacaac caccccatga caacgggacg
      241 ccgcccgcgc tattctgtcg atatcttgac ctgggaggct aacaatgaac acagcacaga
      301 aacagacggg ttggggtttt gcggcgcgtt gggtggcgat cataacggtg actttcctca
      361 tcgggaccgc ctttgccttc gcgtccatgt ggtcgtttgg cggcccaata ttggaaaggc
      421 tcgacgcgcg gatcgccggg cctgtctccg gcgtgtgggt cggcgcgctg atgggtctgg
      481 ggttgggcgt cggccaggcg attgccttgc gcggccgcgg catcagcccg acgcgctggg
      541 tggcgagcag caccctcggc agcgcggccg gattcgccct gttcgtcgcc ctgacggccc
      601 aagggggaac gcagcgaaca cagcttgccc tcctggccgg ggggatggcg gggctgggca
      661 tcggtgtcgc ccagtggatg cagttgcgag gccgcctgag caacgccatc atctgggttc
      721 cggtgacggt ggtggccttt ttgccggtcg ccctgctggc ctacaacggc agtgaggtaa
      781 acgcgggact ggtcatgctg gcgatgggcc tggccgtcgc atgtatcacc gggttgggcg
      841 caatgtggtt gttcggcaaa ggaagcccgg cgattgccgc acaggttagc gtcttgttgc
      901 ttttcggtct gtcactcgcg gcgtgcggat cgtcgtcaca gtccgacatc ccgctgccgc
      961 caaccatcag cccggacgca caccgcatct tccacaacgg cgtcgtgctg acgatgaacc
     1021 ccgaccggcc gtcggccgag gccatcagcc ttgccgggga caggatcgag gcagtgggca
     1081 gcgatgccga cgtgctggcg ctgcgccgcc cgaactcggt cgtcgtcgat ctgcaaggcc
     1141 ggacgctcat gcccggcttt gtggatcccc atacccatat cttcaatgac gccgagcaat
     1201 atctggacat gagcgtggac gaggtccagc aggtggcgct ggagaacggg attaccacta
     1261 tcggcgatat gtacgtcacc gaggatttcc tgggggagat gcaagagatg gagcgcgccg
     1321 ggcaattgca aatccgcacc agcctctacc tggtcatgac cgatccttgc ggcgagtcgc
     1381 aaggtgactg gtggcaggaa tatcccccga cgagaacacc gggcgaacaa ctgcgcatcg
     1441 gcggcgtgaa gatattcacc gacggcgggg tatgcaagaa tccggggttg agctacgagt
     1501 tggagccggg cggaggattg ggcgatctgt tccaggctca ggaagaactc aacgggctgg
     1561 tggccgatgc gcaggctcgc gggttccagg tggtcatgca cgcctgcggc gaccgggcca
     1621 ttgatcaggc ccaaaatgcg attgcggcgg cgctgggcgg gcaacccaat actttccgcc
     1681 atcgcatcga tcacaattgt gtcattcggc cggaacagct ggcccgctat ggcgaaatcg
     1741 gtatcgtgcc ggcggtcttc ggcccctatg ccctctgcga acccttcggc ccgcccccgc
     1801 cgccggagta tcaggcgtgg gaatggccct ggcgcgcgct gctggacgcc aatccggggt
     1861 taccggtggc ctggcacggc gacgacccgt ggttcggccg catccgcccg ctggacgacc
     1921 tgtacagtct ggtgacgcgc aacgaggtgg gcgaggacgg ccgtgtttgc gaggcccccg
     1981 actggcaaaa agcccacacc gtcaccgtgg ccgaggccct gccgatgatg acgatcaatg
     2041 ccgcctacgc ccttttccgc gatgaagaag tgggcagtct ggagccgggg aaatacgccg
     2101 acctgatcgt ggtgaccggc aacccgatgg cgattgagcc ggaagcgatt cgagagttga
     2161 acgtcgcgct gacgatgatt ggcggccggg ctgtctactg tgcggatgat agccaaaatc
     2221 tctgtcccac ctctgaaaca ccgactgaat aatcgacgcc ggtttctaca tgatataatc
     2281 gacatcgact acgggaacaa cagtatggcc catgcgcaag aacagccggc gatcacctca
     2341 cccatccact caagccggtg gcgcatatgg ctggcccgcg ccggctggct gctcatcttt
     2401 gccctcatca ccggcatctt catcgccagc gcaccttcgg cccggtacaa cgaattaagc
     2461 ggctggcaat tcggttacgc cgcacaagcc atacggccgt acatacacgt ctatacgttc
     2521 gcagccatcg tcgtcgcttg ccgtctggcc gtagtggcca tcttctacgc cgtcgcactg
     2581 ctcattgctt ttcgcaagtg gggcgactgg tttgccctgt tcgtctcggc cgcgttgctg
     2641 cttgtcgtct ggggttttgg cgctcgcagc gacacctcat cctacgtatt ccctatatgg
     2701 ctcagtgagg ttgaacccct attgaccggc ctcctgaatc tgctctttgt ggcgagttgg
     2761 gttctgctgt tctaccttct gccagacggc cgattcgtcc cgcgtcgagc cggctggctg
     2821 acgctcctca tcatgccggg cctgctcctg tccactctca gctcttattc tgccattccc
     2881 tatcaatatc gcatccggat tgccgaattt gcctggggca tcctttttat cggcatactt
     2941 atcaccatcc tcgtcgggct ttacttccag ctttatcgct atcgcaacgt cgccacgccc
     3001 gcgcaacgcc agcaaatgaa atggattctc gtcggcctgg ccgcccagcc cttgccgctg
     3061 ttgttcggca tcaccgggct tggcggttcc tgggaggcga tgttggcgat ctttgttccg
     3121 ctctttgtcg tcaccctgct gcccgtcacc atcggcctgg ccgtccttcg ccgccgcctg
     3181 tgggacgtcg atccgctcat caatcgctcg ctggtctatg gcgggctgac gctgctggtg
     3241 ttggtcatct atatcgcgct ggttggtctg gctttcgtcc tgtttggcga gagatcgaat
     3301 ctgttgttgg ccgcgcttgc ggtcggcgtg gccgcgctgc tggcccaacc tattcgcgcc
     3361 cgactgcaac aggcagtcaa ccgtctgatg tatggcgacc gcgacgatcc gatcacggtc
     3421 atcagcgagc tggggcggca actggagcat gccgccgcgc cgggcgactc gctggcgacg
     3481 ctggtcgaga ccatcggccg atcgctcaaa ctaccctacg tcgccatcgt cagacaggag
     3541 aatgacaacc acgatctcgt cgccgaggtc ggaacctcgc agccggactt gctgcgattg
     3601 ccgctgatct accagtcgca gcaggtcggc gaactgctgg tggcgccgcg cgcgccgggc
     3661 gaatcgttca ccgctgccga gcgctacctg ctggataacg tcgcccgaca ggccggcgcg
     3721 gccgtctatt ctgcccaact gaccgaccat ctccagcgca gccgcgagca actggtgctg
     3781 gcccgcgagg aagagcgccg ccgattgcag cgtgatctcc acgacggcct cggcccgcaa
     3841 ctggcgacgc tgagcatgca gctcgacgtg gctcgccggc tggccgccga aggctcgccc
     3901 gaagcggccg aacttctggg ccggctttcg ggcgagacac aggcagccat cgccgatata
     3961 cgccgcctgg tgtatggcct gcgcccaccc gcgctcgatc agttaggatt ggccggagcc
     4021 ttgcgggaat acgtggccgg gcagaatcag gggacaagcg ggacgcgcgt caccgtcgaa
     4081 gcaccggacg ctctgccgcc actaccggcg gcggtcgagg tggccgccta tcgcattgcc
     4141 caggaagccg tcgccaactg cctgcgccac gcccacgcta ccgaatgcct aatcaacctt
     4201 aacgtgaggg aagagctgag cctggaaatt agggataacg gccgcggtct gccgtcggcg
     4261 gtcggcggtc gggaatccgc cttcggcgtc ggcctggtct ccatgcgcga gcgcgcggcc
     4321 gaactgggcg gcgtgtgccg catcgaatcc gcgcccggcg gcgggacgcg ggtcatggcg
     4381 atttttccat tgctacagga gccacaacca tgaccgagac aatctccgtt ttcatcgccg
     4441 acgaccaccg cctgttccgt gacggactgc gggcgctgct ggctccgttg gcggacatgg
     4501 ccgtggtggg cgaggcggca aacggcgagg agtccgtggc actggtcgag cagttgcagc
     4561 ccgatgtggt gctgatggat atccagatgc cggcgctcaa cggcatcgac gcgacacgac
     4621 gcatcaccgc tgccagcccc cacatgggag tgatcatcgt gacgatgttt gaggacgacg
     4681 actcggtgtt cgccgccatg cgcgccgggg cgcggggtta cattctgaag ggcgcggggc
     4741 aggaggaggt gacgcgcacg attcgggccg tggcccgggg cgaggcgctc ttcggcccgg
     4801 ccatcgccgc ccggctcatg tcctatttct ccgacccgcg gccgtcggcc ccggccgaac
     4861 tgttccccga actgaccgtt cgcgagcggg aggtgctgaa cctcatcgcc gccggccaca
     4921 acaacgaggc catcgccgcc cggctagtcg tcagccgcaa gactgtgcgc aaccatgttt
     4981 ccaatatatt cagcaagctc caggtggtcg gccgggccga agcgatcatc cgcgcgcgag
     5041 acgcggggat gggggatgcg aattcctaat tcctaattta tactactctt catgacaacc
     5101 acaccccgcc gccgcgtcgt catcaccggc ctcggcacac tcaaccccct cggcaac
//
LOCUS       DAOMQG010000242         5146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6807_length_5146_cov_6.917109, whole genome shotgun sequence.
ACCESSION   DAOMQG010000242 DAOMQG010000000
VERSION     DAOMQG010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6807_length_5146_cov_6.917109"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..552)
                     /locus_tag="PLC06_12105"
     CDS             complement(148..552)
                     /locus_tag="PLC06_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRO91234.1"
                     /translation="MKKLYVGNLPFDTDEEQVRELFSTYGDVKSVDMINDRETGRFRG
                     FCFVAMDNSEADAAIQALNGYSMGGRPLKVNEAKPREERPGGTGGGGGRGGYGGGRPD
                     NRGGGGGRGGYGGGGGRGGDRDRDRGGDRNRW"
     gene            complement(580..1065)
                     /locus_tag="PLC06_12110"
     CDS             complement(580..1065)
                     /locus_tag="PLC06_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRO91235.1"
                     /translation="MAVADGLVVSLAYTLRLDDGEEIDSAASDEPLVYLHGAHNIIPG
                     LEQALTGLKIGDTRTVSVEPGDAYGDFDPDAFELVPYDAFPGDVDLEEGMGLHMIDNE
                     TGRQIEAYISELRDDGAVLDLNHPLAGETLHFDVEIVGLRRATSDEIAHGHAHNADHT
                     H"
     assembly_gap    1288..1387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1620..2555
                     /gene="ppk2"
                     /locus_tag="PLC06_12115"
     CDS             1620..2555
                     /gene="ppk2"
                     /locus_tag="PLC06_12115"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRO91236.1"
                     /translation="MSKKGKKNGKDQEQAAETPEVIIDTAAPEAIPDGDWAAAPDDDK
                     ARDPRFTRTGKLRREYYEAELSKLQKELVKLQYWVKERGLRVGIVFEGRDAAGKGGVI
                     KRITETTNPRVIRVVALGIPNNREKLQWYFQRWVAELPAAGEVCLFDRSWYTRAVTEY
                     VHGFCTEEEYREFLASCPQFERMLMRSGMILLKYWFSVSDEEQERRFQSRAADPKRRW
                     KLSPMDIKSRDMWVEYSQAKDRMFDFTDTKQSPWFVVNADDKRRARLNCISHILSQIP
                     YEDILPPAIELPPRHSPGGYIRPPIDQQTFVPEKY"
     gene            2597..2875
                     /locus_tag="PLC06_12120"
     CDS             2597..2875
                     /locus_tag="PLC06_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRO91237.1"
                     /translation="MVNSVVLLRVGRGLVNEVAQQLADINGVAEVYSVGGRYDLVAIL
                     RVRDNDALAALVTERMLKVDGITDTETLIAFRAFSRHDLEAMFSLGLE"
     gene            3206..4468
                     /locus_tag="PLC06_12125"
     CDS             3206..4468
                     /locus_tag="PLC06_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRO91238.1"
                     /translation="MDDITPKTNGGRVSAWLSGALLLIMLLLFFAGGIMFDRIVLRGF
                     VFGNDHEGFNEALLDEARAIIQNNFVDREAATTDRLQDGALAGMVEILGDTGHSRFMT
                     PVMVEEQHNYTAGEFEGIGAYVEMRDGFVTVVSPIDNSPAQAAGVQPGFIVLEVAGED
                     MTGKTLQEVVDRILGPAGTEVTITFFDPETAENVTLTIKRARIELENVTWTMLPGSTI
                     AYTRVAGFSQDVGKELGAAIDAAEAQGATGVILDLRNNPGGLLHEAVAVSAQFLPARS
                     VVVLRQDSQGQIKEELVPNDADPTSLPLVVLINQGSASASEIVSGALQDAARATLVGE
                     TTFGTGTVLNEFPLSDGSAILLATEQWLTPDGRIIWREGIVPDEVVELTGPVRLLTPE
                     TADDLTADELAATEDAQLLRALEILRSE"
     gene            complement(4547..4990)
                     /locus_tag="PLC06_12130"
     CDS             complement(4547..4990)
                     /locus_tag="PLC06_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRO91239.1"
                     /translation="MDVQTELVLVGRLVLAAFLGAVIGYERELRGRSAGLRTFAAVAL
                     GACLFSIISYVVTPDGNETTRIAAQIVTGVGFLGAGVILHGQGHVSGLTTSATLWAAA
                     AVGMAVGFGLYILAVAATLLLVLLLLLRLVPWLDHRAERETQSKE"
BASE COUNT         1042 a         1647 c         1436 g          921 t
ORIGIN      
        1 agccttcaaa ttccggtttt tgccttactt ctgacaaact gtaggatagc tagattcgta
       61 attaaaaacg gtggttgccg gttacccgac aaccaccgtt tggatgcaat ggtctacgcc
      121 gtctgacagt tcagtcaatc aataaaacta ccagcgattg cggtcgccgc cgcggtcgcg
      181 gtcacggtcg ccgccacggc caccgccacc accataacca ccacggccgc cgccgccgcc
      241 tcgattatca ggacgaccgc cgccgtaacc accacggccg ccgccaccac ccgtgccacc
      301 cggacgctct tcgcgcggct tggcctcatt caccttcaac ggacggccgc ccatcgagta
      361 cccgttaagg gcctggatcg cggcatccgc ttccgaattg tccatcgcca cgaagcaaaa
      421 gccgcggaag cggccggtct cgcggtcgtt gatcatatcg accgacttga cgtctccgta
      481 ggtggaaaac aattcacgaa cctgctcttc atccgtgtca aacggcaagt taccaacgta
      541 cagctttttc atcttccttt tcaaacccca aaaataaaac tagtgtgtgt gatccgcatt
      601 gtgggcatgc ccatgggcga tttcatccga tgtcgcccgg cgtaaaccaa cgatctcaac
      661 gtcaaaatgc agggtttccc ctgccagggg gtgattcaaa tcgaggactg cgccgtcgtc
      721 acgcagctcc gaaatgtagg cctcaatctg ccgccccgtc tcattatcaa tcatatgcag
      781 gcccatgcct tcttccaggt cgacgtcgcc cggaaaggcg tcatagggca ccaactcgaa
      841 cgcatccggg tcgaagtcgc cataggcgtc gcccggctcg acactcaccg tgcgagtgtc
      901 accgatcttc aggccggtca gggcctgttc caaacccggt ataatattgt gggcaccatg
      961 cagatagacg aggggctcat cactggcggc cgagtcaatc tcttcaccat cgtcgaggcg
     1021 caacgtgtac gccaggctga cgacgaggcc gtcggcgacg gccatttcct gatccaacga
     1081 atccttagcc aaacaatctc ctaagccggc gtgggccaac aatatcgacg caacccgcgt
     1141 cgtggtctgc tcgcacaaaa aaaccgcccc gacccgtcca cgtcaccgtg acaagtcctg
     1201 gcggccgcgt ttcccgtccg agcaactctc acaccagtca actcatacta taacgaaatg
     1261 ccgatagcgt gtcaaacggt atttttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnacc agtcaactca tactataacg aaatgccgat agcgtgtcaa acggtatttt
     1441 tccctatttt gagtgacgag tgacgatgac caccgacgac agaccacgga ccacagcacg
     1501 tccgtggtct gtcgtccgtc gtcggccttc ttcacccgtc actcgtcgct cgtcactcgt
     1561 aactctggcc gcccgccccc aaatgcgata caacatgatt gagttcccgg aggtgtgcga
     1621 tgagcaaaaa aggcaagaag aacggcaagg atcaggagca ggcggccgag accccggagg
     1681 tcatcattga cacggccgcg ccggaggcaa tccccgatgg cgactgggcg gccgcgcccg
     1741 acgacgacaa ggcgcgcgat ccccgcttca cccgcaccgg caagctccgg cgcgagtatt
     1801 acgaagcgga gttgtcgaag ctccagaaag agttggtcaa gctccagtac tgggtgaagg
     1861 aaagagggct gcgcgtcggc atcgtcttcg aggggcgcga cgcggccggc aaggggggcg
     1921 tcatcaaacg tatcaccgag accaccaacc cgcgcgtcat ccgcgtggtg gccttgggca
     1981 tccccaacaa ccgcgagaaa ctgcaatggt atttccagcg ctgggtggcc gagctgcccg
     2041 ccgcgggcga ggtctgtctg ttcgaccgca gctggtacac gcgcgccgtc accgagtatg
     2101 tccatggctt ctgcaccgag gaggagtacc gcgagttcct ggcgtcctgc ccgcagttcg
     2161 agcgaatgct catgcgctcc gggatgatcc tgctcaagta ctggttctcg gtcagcgacg
     2221 aggagcagga gcgccgcttc cagagccgcg ccgctgaccc gaagcgccgc tggaagctca
     2281 gtccgatgga catcaagtcg cgcgatatgt gggtggagta ttcccaggcc aaggatcgca
     2341 tgttcgactt caccgacacg aagcagtcgc cgtggttcgt ggtgaatgcc gacgacaagc
     2401 ggcgcgcccg gctcaactgc atcagccaca tcctcagtca aatcccgtat gaggacatcc
     2461 tgcccccggc catcgagctg ccgccgcgcc attcgcccgg cggctatatt cggccgccca
     2521 tcgaccagca gaccttcgtg cccgaaaagt attgagggta caatcggggt caattgttaa
     2581 aaaggagagt gacggcatgg taaattcggt tgtcttgctc agggtcggcc gcggcctggt
     2641 caacgaagtg gcccaacagt tggcggatat caacggcgtg gccgaggtct attcggtcgg
     2701 cggccgttat gacctggtcg ccatcctgcg cgtgcgcgat aacgacgccc tggccgcgct
     2761 ggtgacggag cgcatgctca aggtggacgg catcaccgac accgaaaccc tcatcgcctt
     2821 ccgcgccttc tcccgccacg acctggaagc gatgttctcg ctcgggctgg agtgagagaa
     2881 ttacgaatta cgaattacga attacgaatg aagagtaaga gcatccacag atttcacaga
     2941 tgaagaatgg ctcttcaaaa tctgtgaaat ctgtgcaatc tatggattct tcccctcttc
     3001 cattcgtaat ttccttaagc ttcgcataag gttgtccgca tttcccctta aagtcgtcat
     3061 actcctgatc gagtatgcta ttgatgttgg ttaacgaaat ccggggcaga gacccaatcc
     3121 aaactccttt tgcctgttga ctgacccttt gttcatcgcc tgaacctgcc ccggatttcg
     3181 taagcaaaga aaggaatcat tgcagatgga tgatatcacc cccaaaacaa acggcgggcg
     3241 cgtctcggca tggctgagcg gcgccctttt attgatcatg ctgctgctgt tcttcgccgg
     3301 cggcatcatg ttcgaccgca tcgtgctgcg cggcttcgtc ttcggcaacg accacgaagg
     3361 gttcaacgaa gcactgctgg acgaagcgcg ggcgatcatc cagaacaatt tcgtggaccg
     3421 cgaggccgcc accaccgacc ggctgcagga cggcgcgctg gcgggcatgg tcgaaatcct
     3481 cggcgacacc ggccacagcc gcttcatgac ccccgtcatg gtcgaggagc agcacaacta
     3541 caccgccggc gagttcgagg gcattggggc ctatgtggaa atgcgcgacg gcttcgtcac
     3601 cgtcgtcagc cccatcgaca actccccggc ccaggcggcg ggcgtccagc ccggcttcat
     3661 cgtccttgag gtggccggcg aggacatgac cggcaagacg ctgcaagagg tggtcgaccg
     3721 catcctcggc ccggccggca ccgaggtgac catcaccttc tttgaccccg aaacggccga
     3781 gaacgtgacc ctgaccatca agcgggcgcg gatcgagctg gagaacgtga cctggacgat
     3841 gctgcccggc tccaccatcg cctatacccg cgtcgccggc ttcagccagg acgtgggcaa
     3901 ggagttgggg gcggccatcg acgcggccga agcgcaaggg gccacggggg tcatcctcga
     3961 cctgcgcaac aatcccggtg gcctgctgca cgaggcggtg gccgtgagcg cccaattcct
     4021 gcccgccagg tcggtcgtcg tcctgcgcca ggattcgcaa ggccaaatca aggaagagct
     4081 tgtgcccaac gacgccgatc cgacgtcgct gccgctggtg gtgctcatca atcagggcag
     4141 cgccagcgcc agcgagatcg tctccggcgc gctgcaggac gccgctcgcg ccacgctggt
     4201 gggcgagacg accttcggca cgggcacggt gctcaatgaa ttccccctgt ccgacggctc
     4261 ggccattctg ctggctacgg agcaatggct gacgcccgac ggccgcatca tctggcgcga
     4321 gggcatcgtc cccgacgagg tggtggagct gaccggcccg gtgcgcctgc tgacgccgga
     4381 aacggccgac gacctgacgg ccgacgagct ggccgccacg gaagacgcgc aactcctgcg
     4441 cgcgctggag attttgcgga gcgagtaatt tcatcccaga cctgacaggt gggcgcggtg
     4501 acgctttttc tccatccgat accctggcgc gcccacctgt caggtctcac tctttcgact
     4561 gcgtctcccg ctccgcgcga tgatccagcc agggcaccag gcgcagcagc agcagcagca
     4621 cgagcagcaa cgtggccgcc acggccaaaa tatacagccc aaagccgacg gccatgccca
     4681 ccgccgccgc cgcccataat gtagccgaag tggtcaggcc gctgacgtga ccttgtccgt
     4741 gaagaatcac ccccgcgccc aaaaagccga cgccggtgac gatctgcgcc gcgatgcgcg
     4801 tcgtctcgtt gccgtcggga gtgacgacat aggagatgat cgagaacagg caggccccca
     4861 gcgccacggc ggcgaaggtg cgcagcccgg cgctgcggcc gcgcagctcc cgctcatagc
     4921 cgatgaccgc ccccaaaaac gcagccagca ccagccgccc caccaacacc aactccgtct
     4981 gtacatccat ttatacgcac ctccagtgga ttccctttct cttcattcgt aattcgtaat
     5041 tcggtcattc ccccatccat cctggcagcg aatcgagcag gcgggccatg atcgccgcgg
     5101 tgcgggttac gaacagttcg gggcggccgt aggcctcgcc gtcctt
//
LOCUS       DAOMQG010000243         5142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6819_length_5142_cov_4.749558, whole genome shotgun sequence.
ACCESSION   DAOMQG010000243 DAOMQG010000000
VERSION     DAOMQG010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_5142_cov_4.749558"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3719)
                     /locus_tag="PLC06_12135"
     CDS             complement(<1..3719)
                     /locus_tag="PLC06_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91240.1"
                     /translation="MDVLSTFLPNDRRHALAEGRPLPDRATGAALFADISGFTALTAA
                     LRRELGARRGAEEVIRHIGVVFTELIDRIHRYHGNVIGFSGDAITCWFGDDDDGGGSS
                     RAVAAAFALQAVMARIETITTPAGLAIPLAIKVAVATGPARRFLVGDPHTYVIEIIAG
                     ATVDRTAAAERLADRGEVIVDAATVAGLTNPPVVAAWRQSEGERYAVLSSPGDQPLPP
                     EPPEPPDIDNEAARSWLLPPVCRHLMQGGETFLAELRPAVPFFVRFGGIDYDGDDEAE
                     QKLDAFVRRVQTVLDRYEGFLVQITMGDKGSYLYICFGVPIAHENDPARAMSAALAMR
                     ETAAELPWLDPLQIGISRGLVQSGAYGGSRRRTFAAMGNEVNITARLMTHARPGQILV
                     SARMVEAAGAELIANPLPPIQLKGVDEPFAVYELVGRSTAPRGSAAAPATKMVARAAE
                     RAQLDEALAELVDGRSRAVLIEGAAGMGKSLLMAELAHKAAAVPGALVLAGGGDAIEQ
                     STPYFGWRPVFEALLDIHHRPDWRDELAARLDPAARPFLPLLNAVLPLDLPDNDITAQ
                     LAGEARQDNTQELLLGLLRSVAGGSPLVVLLDDAHWLDSASWALADRARREVAPLLLA
                     LVSRPLGDDAPAAYAEIAAAPGTRRITLDALSPVDIEALVALRLGVAGLLREIADLIQ
                     EKAEGHPFFSEELAFALRDAGLLRVSGGVARVSPGADLTALDFPTTIQGIITSRIDRL
                     SASQQLTVKVASVIGRIFALRVLTGVYPVAEAEAQLRADLTRLERLDITPLETPEPNL
                     AYIFKHIVMQEVVYSLMTTDQRRRLHRNTAEWYERAAEGDLSRNFGLLGHHWRLAGES
                     GKAIDYFEKAGENAFRDFANREAIRFLEQALELSGPDIAPLRRARWHRLLGEARYRLT
                     QIEASTADFNEGLALLGREVPSSAVGRGAGLMAELTRQAGHRSPAWRWVHRDGGDRRE
                     ALLEAARAMEGMAEIDYNLGDFLAAFYCTMRAFNLAEKAGGASPELMRGYGNMCSLMG
                     AVSLNGAADGYRALALGVEPQIDDLSARAWSRISLSTHSVWVGNWDRAEKEITEALAI
                     YAQLGDWRRWGVAAWLWPQVAQSRGDLRRARDLWAELYEVASRSRDTRHQVRGRGGQF
                     FNFLALDEPVAARNCLATIEELFAANPEMMPVEERLWHAARAAWALHEGDEALAATEA
                     QETLAAIGRARFKFDLIDIFATPAEVLPALRERGKP"
     gene            complement(3887..>5142)
                     /locus_tag="PLC06_12140"
     CDS             complement(3887..>5142)
                     /locus_tag="PLC06_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRO91241.1"
                     /translation="DETRMIVSVDMRRMDAIEWIDRENMRACVQAGITGKHLEERLAA
                     QGLTCGHEPDSVELSTLGGWIATNASGMKKNRYGNIEDIVENVTLVTPEGVVEHLSNM
                     PRVSMGVEPVRLAFGSEGNLGLITRAVLKIHALPEVKEYGSYVFRDFETGTAFLYDLM
                     RTGALPASIRLVDNVQFRFGQALKARPTGQEKLMSRIQQTVLESVKGFDLRQMSAATV
                     VMEGSAAEVAYQKAEIKRIAARHGGVSGGSHNGQRGYMLTYAIAYIRDFLTDYHIIGE
                     TYETTVPWDRIHDVISAVEKRATEMHREFGLPGKSYVSPRITQLYHTGVCIYFTHGFS
                     TRGVENPDRAFAAIEHAMRETIMANGGSISHHHGVGKLRRDFVPQTLTPAAVDLLREV
                     KAAADPGNVFGIGNNVFAMEKERDI"
BASE COUNT          754 a         1742 c         1833 g          813 t
ORIGIN      
        1 ggcttgcccc gctcgcgcag cgccggcagc acctcggccg gggtggcgaa aatgtcgatc
       61 aggtcgaact tgaagcgcgc ccggccgatg gccgccagcg tctcttgcgc ctcggtcgcc
      121 gccagcgcct cgtctccttc gtgcagcgcc cacgccgccc gcgccgcgtg ccacaaccgc
      181 tcctccaccg gcatcatctc cgggttggcg gcgaacagct cctcgatggt ggccaggcag
      241 ttgcgcgccg ccaccggctc atccagcgcc aggaagttga agaactggcc gccgcggccg
      301 cgcacctgat ggcgcgtgtc gcggctgcgg gacgccacct catacagctc cgcccacagg
      361 tcgcgcgccc gtcgcaaatc gccccggctc tgggccacct gcggccacag ccacgccgcc
      421 accccccagc gccgccagtc gcccagttgc gcatagatgg ccagcgcctc ggtgatctcc
      481 ttctccgccc gatcccaatt gccgacccag acgctgtggg tggagagcga gatgcggctc
      541 cacgcccgcg ccgacaggtc gtcgatctgc ggttccacgc cgagggccag ggcgcggtag
      601 ccgtcggccg cgccgttgag cgacaccgcg cccatcagcg agcacatgtt gccgtagccg
      661 cgcatcagct ccggcgacgc cccgccggcc ttctcggcca gattgaaggc gcgcatggtg
      721 caatagaacg cggccaggaa atcgcccagg ttgtagtcga tctcggccat cccctccatg
      781 gctcgcgccg cttcgagcag cgcctcgcgc cggtcgccgc cgtcgcgatg aacccaacgc
      841 cacgcgggcg agcggtggcc tgcctgccgc gttagctcgg ccatcagacc cgcgccccgc
      901 ccgacggccg acgacggcac ttcgcggccc agcagcgcca gcccttcgtt gaaatcggcg
      961 gtgctcgcct cgatctgcgt cagccgatag cgggcttcgc ccagcagccg gtgccagcgc
     1021 gcccgccgca gcggggcaat gtccggcccg ctcaactcca gcgcctgctc caggaagcgg
     1081 atcgcctcgc ggttggcgaa gtcgcggaag gcgttttccc cggccttctc aaaatagtcg
     1141 atggccttgc ccgactcgcc ggccagccgc cagtggtggc ccagcaagcc gaagttgcgc
     1201 gacaggtcgc cttcggccgc gcgctcatac cattcggccg tgttgcggtg cagccggcgg
     1261 cgctggtcgg tggtcatcag gctgtagacc acctcctgca tgacgatatg cttgaagata
     1321 taggccaggt tcggctccgg cgtttccagc ggggtgatgt cgagccgctc cagccgggtc
     1381 aggtcggcgc gcagctgggc ctcggcctcg gcaacgggat agacgccggt cagcacgcgc
     1441 agggcgaaga tgcggccgat gacgctggcg accttgaccg tcagctgctg cgaggccgac
     1501 agccggtcga tgcggctggt gatgatcccc tggatggtgg tggggaagtc gagcgccgtc
     1561 agatccgcgc cgggcgagac gcgggccacg ccgccgctca cgcgcaataa ccccgcgtcg
     1621 cgcagggcga aggccaattc ctcactgaag aacgggtggc cctcggcctt ctcctggatg
     1681 aggtcggcga tctcgcgcag caggccggcg acccccagcc gcagcgcgac cagcgcctcg
     1741 atgtcgaccg gggacagcgc gtcgagcgtg atgcggcgcg tgcccggcgc ggcggcgatc
     1801 tcggcatagg cggcgggcgc gtcgtcgccc aacggccgcg acaccagcgc cagcagcagc
     1861 ggggcgacct cgcgccgggc gcggtcggcc agcgcccacg aggccgaatc gagccagtgg
     1921 gcatcgtcga gcaagacgac cagcgggctg ccgccggcca ccgaccgcag cagccccagc
     1981 agcagttcct gggtgttgtc ctggcgcgcc tcgcccgcca gttgggcggt aatatcgttg
     2041 tcgggcaggt cgagcggcag cacggcgttg agcaggggca ggaacggccg cgccgccggg
     2101 tcgagtcggg ccgccagctc atcgcgccaa tcggggcggt ggtggatgtc gagcagcgct
     2161 tcgaagacgg ggcgccagcc gaaatagggg gtcgattgct cgatggcgtc gccgccgccg
     2221 gcgagaacca gcgcgccggg cacggccgcc gccttgtggg ccagctcggc catcagcagc
     2281 gatttgccca tgcccgccgc gccctcgatg agcaccgccc gcgagcggcc gtcgaccagt
     2341 tcggccagcg cttcgtcgag ttgggcgcgt tcggcggcgc gggcgaccat ctttgtggcc
     2401 ggggcggcgg cgctgccgcg cggcgcggtc gagcgcccga ccagttcata cacggcgaac
     2461 ggctcgtcta cccccttgag ctggatgggc ggcagcgggt tggcgatcag ctccgcgccg
     2521 gccgcctcga ccatgcgcgc cgagacgagg atttgccccg gccgggcgtg ggtcatcagc
     2581 cgggcggtga tgttgacttc gttgcccatg gccgcgaacg tgcgccgccg cgagccgcca
     2641 taggcgccgc tctgcacgag gccgcggctg atgccgatct gcagcgggtc gagccagggc
     2701 agctcggcgg ccgtctcgcg catggccagc gcggccgaca tggcccgcgc cgggtcgttc
     2761 tcgtgggcga tgggcacgcc gaagcagatg tagagatagc tgcccttgtc gcccatggtg
     2821 atctgcacca ggaacccctc gtagcggtcg aggacggtct ggacgcggcg cacgaaggcg
     2881 tcgagcttct gctcggcctc gtcgtcgccg tcgtagtcga tgccgccgaa gcggacgaag
     2941 aacggcaccg ccgggcgcag ttcggccaga aatgtctcgc cgccctgcat cagatggcga
     3001 cagaccggcg gcagcagcca cgaccgcgcg gcctcgttgt cgatgtcggg cggctccggc
     3061 ggctccggcg gcagcggctg gtcgccgggg gacgacagga cggcgtagcg ctcgccctcg
     3121 ctttggcgcc acgcggcgac gaccggcggg ttcgtcaggc cggccaccgt cgccgcgtca
     3181 acgatgacct cgccccggtc ggccagcctc tcggccgcgg cggtgcgatc gacggtggcc
     3241 ccggcgatga tctcgatgac gtaggtatgg ggatcgccga cgagaaagcg gcgagcgggg
     3301 ccggtggcca cggcgacctt gatggccagg gggatggcca ggcccgcggg ggtggtgatc
     3361 gtctcgatgc gcgccatgac cgcctggagc gcaaaggcgg cggcgacggc gcgactgctc
     3421 ccgccgccgt cgtcgtcgtc gccgaaccag caggtgatgg cgtcgccgct gaagccgatg
     3481 acgttgccgt ggtagcggtg gatgcggtcg atcagttcgg tgaagacgac gccgatgtgg
     3541 cggatgacct cttccgcgcc gcgtcgggcg ccgagttcgc gccgcagggc ggccgtcagg
     3601 gcggtgaagc cggagatgtc ggcgaacagg gccgcgccgg tggcccggtc gggcaggggg
     3661 cggccctcgg cgagcgcgtg ccggcggtca ttgggcagga atgtggagag gacgtccatg
     3721 tgtcgatgtg accgggagga gaagttctta tattgtagcg caatctgtca gattgcgtcc
     3781 ggcttcccaa gacgcaatct gacagattgc gttacaggtt acagacggca atctgtcaga
     3841 ttgcgttgcg ggttacagac ggcaatctaa aagattgcgt tacaggttat atatcgcgct
     3901 ctttttccat cgcaaagaca ttgttgccga tgccgaacac gttgccgggg tcggcggcgg
     3961 ccttcacttc gcgcagcaga tcgacggccg cgggggtcag cgtctgcggc acgaagtcgc
     4021 ggcgcagttt gcccacgccg tggtggtggc tgatggagcc gccgttggcc atgatcgtct
     4081 cgcgcatggc gtgctcgatg gccgcgaacg cccggtcggg attttccacg ccgcgggtcg
     4141 agaagccatg ggtgaagtag atgcacacgc cggtgtggta gagctgggtg atgcgcggcg
     4201 acacgtagga tttgccgggc aagccgaact cgcggtgcat ctcggtggcc cgcttctcca
     4261 cggcgctgat gacgtcgtgg atgcgatccc atggcacggt cgtctcgtag gtctcgccga
     4321 tgatgtggta gtcggtcagg aagtcgcgga tgtaggcgat ggcgtaggtg agcatgtagc
     4381 cgcgctggcc gttgtgggag ccgccggata cgccaccgtg gcgcgcggcg atgcgcttga
     4441 tctccgcctt ctgataggcc acctcggccg ccgacccttc catgacgacg gtcgccgccg
     4501 acatttggcg caggtcgaac cccttgacgc tctccagcac ggtctgctgg atgcggctca
     4561 tcagcttctc ctggccggtc ggccgcgcct tcagcgcctg gccgaagcgg aactggacgt
     4621 tatcgaccag ccggatgctg gccggcagcg cgcccgtccg catgagatca tacaggaagg
     4681 cggtgcccgt ctcgaagtcg cggaagacgt aggagccata ttccttcact tccggcaggg
     4741 cgtggatttt cagcacggcg cgggtgatga ggcccagatt gccctcgctg ccgaaggcca
     4801 gccgcaccgg ctcgaccccc atcgacacgc ggggcatatt gctgagatgt tcgacgaccc
     4861 cttcgggcgt gaccagcgtc acgttctcga cgatgtcctc gatgttgccg tagcggtttt
     4921 tcttcatgcc gctggcgttg gtggcgatcc agccgccgag ggtggacagt tcgacgctgt
     4981 cgggttcgtg gccacaggtg aggccctggg cggccaggcg ctcttcgagg tgcttgccgg
     5041 tgatgccggc ctggacacag gcgcgcatgt tctcgcggtc gatccactcg atggcgtcca
     5101 tgcggcgcat gtcgacggag acgatcatgc gcgtctcatc gg
//
LOCUS       DAOMQG010000244         5141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6821_length_5141_cov_12.263272, whole genome shotgun sequence.
ACCESSION   DAOMQG010000244 DAOMQG010000000
VERSION     DAOMQG010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6821_length_5141_cov_12.263272"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1134
                     /locus_tag="PLC06_12145"
     CDS             22..1134
                     /locus_tag="PLC06_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRO91242.1"
                     /translation="MSGNQKGLSLQVIVIILVLLAIFFGWLGYQPGTLLNEYARGLGL
                     TLFGVALVVLLTDSLGKRREQQLLAYLEEQRRAQEDAQLTRESLLAKTQLIREVASGD
                     SALAERAIRELDAKGWLTDGTLSNTHLMGANLAGANLGWVNLSGAFLSNINLQDADVS
                     YGNFSAASMPDAKMARANLDMANFTEADLRNAELSLCSMNETDLSGAQLEGAILSGAT
                     MVEVNLSKVQGERIALNGANLERANMVESVLSHANLERATLTGANLGWANLSRANLEK
                     ANLAEANLSGANLDEARLNGANLSGAFLFGTRVSLKSLSTAKSLANATMPDGVKYEEW
                     IRRQSGEYVPAATTSLGGSAGSDYDSPLDYTPTQEI"
     gene            complement(1393..2646)
                     /locus_tag="PLC06_12150"
     CDS             complement(1393..2646)
                     /locus_tag="PLC06_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HRO91243.1"
                     /translation="MTIQPIDPIVLREQFPALRRTINGQPAVFLDGPGGTQAPERVIE
                     AMTGYLRSGSSNHGGPFQTSRETDAATDSAREAMRDMLNANRAEEIVFGQNMTSLTFS
                     ISRAIAQEWQPGDEIVLTRIDHDGNVSPWLRAAEDRGVTVRRLDFRPENCTLAIDTLP
                     GLLNDKTRLVAVNYASNAVGTINDVAKVVEMAHAVGALVYVDAVHYAPHDVVDVQALD
                     CDFLACSVYKFFGPHTGVLYGKYEHLDRLNAYRVRPAGDIPPGKWETGTQSFESLSGV
                     TAAIDYLASLGGQDGTRRERIARGMKAIKQYEATLSERFLQAATRVPGIRVYGITDPE
                     ELERRTPTFAISLAGFSPEYVATYLGDRGFFVWHGHYYAVEVMARLGLLESGGLVRIG
                     FTHYNTYEELDRLIEALIELAATAQ"
     gene            2798..3643
                     /locus_tag="PLC06_12155"
     CDS             2798..3643
                     /locus_tag="PLC06_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91244.1"
                     /translation="MRRSTVSLILILVVMLLTGLVLTTSRRVEASGRFAQSGSSDSVA
                     YLPVIFSTRKGPTFDMAVFMIGDGRLYEVMHNSGSQARHQTQTSAARFYHTKGNEIKA
                     EWEELWVGGNLIFRGTDTSPGNDQYYTLYENGLAGSAWSPRYWSVGEVYERNPFVVFY
                     RKGDCGVVASGYQRSWLRFEGYYPTYTFKSGLKLDDVVELTWLLQEDGQPVESYFYAR
                     SYGLVGWGSADRGYSYINEIHAPGQRPDNKREVIDCLDDRSFHFLQFGPELNFGPLPP
                     GFRAK"
     gene            complement(4095..4391)
                     /locus_tag="PLC06_12160"
     CDS             complement(4095..4391)
                     /locus_tag="PLC06_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91245.1"
                     /translation="MALELILDEHDKDISCFVAKIINGQPASVWKYDDKGRLVRDHLT
                     ALLRRQVGEQAPSLFTRVTGMELEDRIPITVGDYARMMQVYGQHIINDDPNLFG"
     gene            complement(4549..>5141)
                     /locus_tag="PLC06_12165"
     CDS             complement(4549..>5141)
                     /locus_tag="PLC06_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91246.1"
                     /translation="DIYDIQQNGLTWTSGLSLAADVAGLALPVVTGGGAAVRALAHAD
                     DVADVVTRVDEAIEVARTVENLGDVGQAADDLPVLAKALDSTNNTPIIIGEDMDRVQR
                     YANQFGGETISDSITGLEWTEKANTAWITDVRATGRQVIDIGPSFERRANRVGQGISP
                     ASRPYEIERRILKGYERYEKAFERAGKYAGGVPGFD"
BASE COUNT         1055 a         1414 c         1495 g         1177 t
ORIGIN      
        1 agacggaggt agttgatcga catgtccgga aaccagaaag gcctgagcct tcaagtcatc
       61 gttatcattc ttgttttgct ggccattttc tttggctggc tgggctacca gcccggcaca
      121 ctgctgaatg aatatgcccg cggcctgggc ctgacccttt ttggcgtggc cctcgttgtt
      181 ttgctgactg actcccttgg gaagcggcgg gaacagcaat tgctggccta tctggaagag
      241 cagcgccggg cgcaggaaga tgcccaattg acccgcgaga gcctgctcgc gaagacgcag
      301 ctcatccgcg aagtggccag tggtgattca gcgttggccg agcgggcaat tcgcgagctt
      361 gatgccaaag ggtggcttac ggacgggaca ctctccaata cccatttgat gggggccaat
      421 ctggccggcg ccaatttggg gtgggtgaat ctgagcggcg cattcctgag caatattaat
      481 ttgcaggatg cggatgtgag ctatggaaat ttcagcgccg ccagcatgcc cgatgcaaaa
      541 atggcgcggg ccaatctgga tatggcgaat ttcaccgaag ccgatttgcg caacgcggaa
      601 ctcagcctgt gctcgatgaa cgagacggat ctatccggcg cccaattgga aggcgccatc
      661 ctgagtggtg cgacgatggt ggaggtgaac ctgtccaagg ttcagggtga gcgaatagcg
      721 ctcaacggcg cgaatcttga gcgggctaat atggtggaaa gcgtcctgtc ccatgccaat
      781 ctggaacggg ccaccttgac cggcgccaat ctcggctggg ctaatctgag tagagccaac
      841 ctggagaaag cgaacctggc ggaggcgaac ctcagcgggg caaacctgga cgaggcgcgt
      901 cttaacggag ccaatctgtc cggcgctttc ctgtttggga cgagggtgtc gctcaagtcg
      961 ctatcgacag ccaagtcact ggcgaatgcc acgatgcccg atggtgtcaa atatgaggaa
     1021 tggattcgcc ggcaatcggg cgagtacgtg ccggcagcta caacgtcact tgggggatcg
     1081 gccgggagcg attacgattc gccgctggat tacacgccca cccaggaaat atagatctat
     1141 tggtattgca aaggctctct accgagctat tgtcatttga aaagtcaaag gccctgtgtt
     1201 gttacacaca gggcctttgc tttgcaccgt agcgctacta actcttacga ggaggtagcg
     1261 ctacggtgca ggtgaataaa taactactca ttaagcacca cctcctcagg ttaaagatgg
     1321 ctgtgcagcg tttgctgcgg atgatgtgcg ggattatggc atatgccggg atgggtgtca
     1381 accgatactc atttattgtg cggttgcggc cagttcgatc aacgcctcga tcagccgatc
     1441 cagttcctca tacgtgttgt aatgggtaaa accaatgcgt accaggccgc cgctttccag
     1501 caaccccagt cgagccatga cttccacggc gtagtaatgg ccgtgccata cgaagaaccc
     1561 tctgtcaccc aggtaagtgg cgacatattc cggcgaaaaa cccgccaggc tgatcgcgaa
     1621 ggtgggcgtc cgccgctcca gctcctccgg atcggtgatg ccgtagaccc gaatgcccgg
     1681 cacgcgggtg gccgcctgca ggaagcgctc gctcagggta gcctcgtact gtttgattgc
     1741 cttcatcccg cgggcgattc gctcccggcg cgtgccgtct tgcccgccta atgaggccaa
     1801 ataatcgatg gccgccgtga cgccgctgag gctctcgaag ctctgcgtgc ccgtttccca
     1861 tttgcccggc ggtatgtcgc cggctgggcg aactctgtac gcgtttaacc gatcgaggtg
     1921 ttcgtattta ccatagagca cgcccgtgtg cgggccgaag aacttataga cggagcaggc
     1981 caggaaatcg caatccagcg cctgaacgtc gaccacgtcg tgaggggcgt agtggacggc
     2041 atctacatac accagcgctc cgaccgcatg ggccatctcc acgactttgg ccacgtcgtt
     2101 gattgtgccc acggcgttgg aagcataatt gacggccacc agccgtgttt tgtcattgag
     2161 gaggcccggc aacgtgtcaa tcgccagggt gcaattctcc ggccgaaagt cgagccggcg
     2221 aaccgtgacg ccgcgatcct cggccgcgcg cagccagggt gaaacgttgc cgtcgtgatc
     2281 gatgcgcgtc aggacgatct catcgccggg ctgccattcc tgggcaatcg cccggctgat
     2341 gctgaaggtc aggctggtca tgttctggcc gaacacgatc tcttctgcgc ggttggcatt
     2401 gagcatgtcc ctcatcgcct cgcgcgcgga atcggtcgcc gcgtcggttt cgcggctcgt
     2461 ctggaagggg ccgccgtggt tgctgctgcc gctgcgcaga tagccggtca tggcctcgat
     2521 gacgcgttcg ggggcctggg tgccgcccgg cccgtcgagg aatacggccg gttggccgtt
     2581 gatcgtgcgc cgaagcgccg ggaattgttc gcgcaagaca atggggtcga taggttgaat
     2641 agtcaaggac ctgctccttt atgatatgtg ttgggattat agcgcatgaa agctgcctga
     2701 ataagaagcg gccaatagtt ggtgtgttgg ttgtttgcct ccataattaa catgcacttt
     2761 cagggttcag gcttatcctg tcctggcaca tgaattcatg agaagatcga ccgtttccct
     2821 gattctgatt ctcgtcgtga tgcttctgac cggtctggta ttgacaacca gccggcgagt
     2881 cgaggcgagc ggccgttttg ctcagtcggg ttcgtccgat tctgttgcct atctgccggt
     2941 gatattcagc acgaggaagg ggccgacttt tgatatggcc gtgtttatga ttggcgacgg
     3001 ccgtctgtat gaggtgatgc acaacagtgg ctcgcaggcc cgtcatcaga cgcagaccag
     3061 cgcggcccgc ttttaccaca ccaagggaaa cgagatcaag gcggaatggg aggagttgtg
     3121 ggtcgggggg aatctgatct ttcgcggcac ggacaccagc cccggcaacg atcagtatta
     3181 taccctctat gaaaatgggc tggccggctc ggcctggagt ccgcgatatt ggagcgttgg
     3241 cgaggtttat gagcgcaatc cgtttgtggt gttctatcgc aagggcgatt gtggggtggt
     3301 cgccagcggg tatcagcgga gctggcttcg ctttgaaggc tattacccga cctacacgtt
     3361 caaaagcggc ctgaaacttg acgatgtggt cgagttgacc tggctcttgc aggaggatgg
     3421 gcaaccggtc gaaagttatt tctatgcccg atcctatggc ctcgtggggt ggggcagcgc
     3481 cgatcgaggc tatagctata tcaacgaaat tcacgctccc ggccagcggc cggacaacaa
     3541 gcgcgaggtg attgattgtc tggacgaccg tagtttccac ttcctgcaat ttggcccgga
     3601 gctaaatttc ggcccgcttc ctcctggatt ccgcgcgaaa taaccgcgcc gcctccggca
     3661 tataataggc tggagattgc tgggttcacc gttgtgtttg gcgtagtgcg gtagttccca
     3721 aacggatcaa accgcgccaa cgaacccgcc acaacgccgc taccctgccc cagagccgca
     3781 atgttgccca ggtggtcggt gtagataaag tagaatgcac ccgcggtcgc gcccaccttc
     3841 gtctgcacgg ccacgatttg tccgccgagc cggtaggttg tgcgcaccgt gtccgctccg
     3901 ctgcccggcg ggtcggtcag ctcgtagtcg gggaaggggg tgtagatgat ggttccgttg
     3961 ggatagaggg tgcgcatgtg gtttccgcta gcgtcgtatt tagttgttca ggtgcgggtt
     4021 ggtcgctgac gcggtgttat cggcgaggcg tcagcaagga aggttagtag tacagcatag
     4081 gttagtcgta caagttagcc aaataagttt ggatcatcat tgatgatgtg ttgtccataa
     4141 acttgcatca ttcgtgcata gtctcctacc gtaatgggaa tccgatcctc taattccatt
     4201 cctgttacgc gagtaaacaa tgacggcgcc tgctcgccaa cttgacgtct gagtaacgca
     4261 gtcaagtgat cgcgcactaa tctaccttta tcgtcatact tccaaactga ggcaggctgt
     4321 ccgtttatta ttttcgcaac gaaacagctg atgtctttgt cgtgctcgtc tagaatcagt
     4381 tcaagggcca agttctcgcc aaggtaaacc actctagttt ggcctatcct gtgcttgatc
     4441 tccagagaag gccccttaaa gcggttgttt aggagtactg aaaagctgtt ttgaacagcg
     4501 tcgaagaata gcttctgaag tctcgtaacc actatatcct ctcaagaatc agtcaaaccc
     4561 cgggacgcca cctgcatact taccggcacg ttcaaaggct ttctcatatc tttcgtatcc
     4621 cttcaatatc ctgcgctcaa tctcatacgg cctggaggca ggtgaaatac cctgcccgac
     4681 cctattggca cgtcgctcaa aactcggccc aatatcaatg acttgtcgac ccgtcgctcg
     4741 cacatctgta atccacgctg tattagcctt ttctgtccat tcgagcccag ttatagagtc
     4801 gctaatggtc tctccgccaa actggttggc gtaacgttgt acacggtcca tatcctcccc
     4861 tatgataatg ggagtattgt tcgtagaatc aagagctttc gctagtacag gtagatcatc
     4921 agcggcctga cccacgtcac ccagattctc tactgtccgc gccacctcaa tcgcctcatc
     4981 aacacgtgtc acgacgtcgg caacgtcatc cgcatgcgca agtgcccgta cggccgcccc
     5041 accacccgtc actaccggca gtgccagccc agccacgtca gcggctaatg atagcccgct
     5101 cgtccaggtc agcccatttt gctggatgtc gtagatatcg t
//
LOCUS       DAOMQG010000245         5131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6838_length_5131_cov_6.716706, whole genome shotgun sequence.
ACCESSION   DAOMQG010000245 DAOMQG010000000
VERSION     DAOMQG010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6838_length_5131_cov_6.716706"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PLC06_12170"
     CDS             <1..1248
                     /locus_tag="PLC06_12170"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRO91247.1"
                     /translation="DVYIDLLTDSGTSAMSDRQWAGMMLGDEAYAGSRNFYHLEEAIQ
                     TYYGYRHILPTHQGRGAEHLISRVAIKPGQYLLGNMYFTTTRLHQEMAGGIFVDVIID
                     EAHDPENLHPFKGNIDLDKVEYQIATHGAAAIAYISLAGTVNMAGGQPVSMANVRDLR
                     ALCDRHGIKLFLDATRMVENSFFIQEREEGYADKSIAEILREFCGYTDGAWMSAKKDN
                     LVNIGGWLAVNDWDLFEELRNLIVVFEGLHTYGGAAGRDMEAMAIGIVESVQDDHARS
                     RVGQVRYLGNLLTEWDVPIVQPVGGHAIFLDARRFYPHIPQDQFPAQTLAAELYLDSG
                     IRSMERGIASAGRDPHTGDHYYPKLELTRLTIPRRVYTQAHMDVTAESVKAVYDARHS
                     THGLKMVYEPKYLRFFQARFERL"
     gene            complement(1320..3128)
                     /locus_tag="PLC06_12175"
     CDS             complement(1320..3128)
                     /locus_tag="PLC06_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91248.1"
                     /translation="MTTTTTPIHPLRRLATYARAYRGRVALATLYSILNKIFDLAPPV
                     LIGMAVDVVVMQQDSILARFGVVDVTHQLVGVGLITAVLWGFESLFDYLKAVGWRNLA
                     QDIQHDLRLSTYDHVQRLDMAYFEEQSTGGLMSVLNDDVNQLERFLDIGANEIIQLVT
                     TIVVVLGMFLVAAPSVTWLTVVPIPFIIYGSLKFQQRLGPRYAAVREQVGILNSFLSN
                     NLSGIATVKSYTAEEYEVGRAAAESDQYRQRNAAAIKLSSAFVPLIRMFIMGGFIAIM
                     VFGGVMVINGQLNVGVYSVLVFMTQRLLWPLTRLGETLDLYQRAMASTGRVFRLLDVQ
                     SNVIEGTATLPAPARGEVDFRDVSFRYSWHDYEGYTDSRPHVLRDLSFHIPAGTTAAI
                     VGPTGSGKTTVIKLLLRFYDVQSGGISLDGRDIRDLTFDALRGAIGLVSQDVFLFHGS
                     VRENIAYGTFDASLDDIVAAARTAEAHEFIMALPDGYDTIVGERGQKLSGGQRQRISI
                     ARAVLKNPPVLILDEATSSVDNETEAAIQRSMERISVGRTMIVIAHRLSTVRNADRIF
                     VLENGELREAGTHDELAHTDSGIYAALWRVQTGEAR"
     gene            complement(3437..>5131)
                     /locus_tag="PLC06_12180"
     CDS             complement(3437..>5131)
                     /locus_tag="PLC06_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91249.1"
                     /translation="VIQLDMSATPRYSKGSLFTWTVFDYPLKQAILDNVVKRPYKGVV
                     RGMAETPSDIASVRYQAYLTAGVERWREYREQLVPLNRKPVLFVMMNSTKDADDVADY
                     LRTKYPAEFAGERLHVIHTNKVGDVSVRDEDDARQIAADIDKPDSPVNCIVSVLMLRE
                     GWDVQSVTVVVGLRPYTAKANILPEQTIGRGLRLMFRGNTEAEPPFRERVDVIGNNAF
                     IEFVEELEKEEEMAFESFEPGKDKLVIETIFPDPAKAEMDITIPELSALLERKRSLAE
                     EIAAIDVGRIKAPTLPRKQGDAAANAFQYEGYDIITLEKLIEREYAIPEVRTSGEVIS
                     YYAKRIAADVKLPSQFAALVPKVREFLALHAFGEPVDLDDPVMIKAISHPVAQHVTVK
                     AFVAILREVVVEERTPELAGNGRRLSECPGYPWSRPIGAADKTIFNKLAADNQFELTF
                     GHFLEKAPDVVRFSKLPRAFGFSITYTDSVANLRYYEPDFVAVTADGVHHLIETKGQE
                     DVDVRHKDGAARLWCRNATDLTGTSWRYIKVPQKEFESLRPNEFEDLIALEPVGLM"
BASE COUNT          901 a         1461 c         1792 g          977 t
ORIGIN      
        1 gacgtctaca tcgacctgct gaccgacagc ggcaccagcg ccatgagcga ccgccaatgg
       61 gcgggcatga tgctcggcga cgaggcctat gccggcagcc gcaacttcta ccaccttgag
      121 gaagcgatcc agacctacta cggctatcgc cacatcctgc ccacccatca ggggcgcggg
      181 gccgagcatc tgatcagccg cgtggccatc aagccgggcc agtacctcct cggcaacatg
      241 tatttcacca ccacccggct gcatcaggag atggccggcg gcatcttcgt cgacgtcatc
      301 atcgacgagg cccacgaccc ggagaatctc cacccgttca agggcaacat cgacctcgac
      361 aaggtggagt atcagatcgc cacccacggc gcggcggcca tcgcctacat cagcctggcg
      421 ggcacggtca acatggccgg cggccaaccg gtcagcatgg ccaacgtccg cgatctgcgc
      481 gccctgtgcg accgccacgg catcaagctg ttcctcgacg ccacgcgcat ggtggagaac
      541 tcgttcttca tccaggagcg cgaagagggg tacgccgaca aatcgatcgc cgagatcttg
      601 cgcgagttct gcggctacac cgacggggcg tggatgagcg ccaagaagga caatctggtc
      661 aacatcggcg gctggctggc ggtcaacgat tgggacctgt tcgaggagct gcgcaatctg
      721 atcgtcgtct tcgaggggct gcacacctat ggcggcgcgg ccgggcgcga catggaagcg
      781 atggccatcg gcatcgtcga gtcggtgcag gacgaccacg cccgctcgcg tgtgggccag
      841 gtgcgctatc tgggcaacct gctgacggag tgggacgtcc ccatcgtgca gccggtgggc
      901 ggccacgcca tcttcctcga cgcgcgccgc ttctaccccc acattccgca agaccagttc
      961 ccggcgcaga cgctggcggc cgagctgtat ctcgattcgg gcatccgctc aatggagcgc
     1021 ggcatcgcca gcgccggccg cgacccccac acgggcgacc actactaccc caaactggag
     1081 ctgacgcgcc tcaccatccc ccgccgggtc tatacccagg cccacatgga cgtgaccgcc
     1141 gaatcggtga aggcggtcta tgacgcccgc cattcgaccc acggcctgaa gatggtctat
     1201 gagccgaagt atctgcgctt cttccaggcg cggtttgaga gactgtaggg gaaattacga
     1261 attacgaatt acgaatgaag agcgcgccct ttttcgtact tcgtacttcg tcctttcccc
     1321 tatctcgctt cgcccgtctg cacgcgccag agagcggcgt agatgccgct gtcggtgtgg
     1381 gccagctcgt cgtgggtgcc cgcttcgcgc agttcgccgt tttccagcac gaagatgcgg
     1441 tcggcgttgc gcacggtgga caggcggtgg gcgatgacga tcatggtgcg gccgacggag
     1501 atgcgttcca tcgagcgctg gatggccgcc tcggtctcgt tgtccaccga tgaggtggct
     1561 tcgtcgagga tgagcacggg cgggttcttc agcacggcgc gggcgatgga gatgcgctgg
     1621 cgctggccgc cggagagctt ttggccgcgc tcgccgacga tggtgtcgta gccgtcgggc
     1681 agggccatga tgaactcgtg ggcttcggcc gtgcgggcgg cggccacgat atcgtcgagc
     1741 gaggcgtcga aggtgccgta ggcgatgttc tcgcgcacgc tgccgtggaa gaggaacacg
     1801 tcctggctga cgaggccgat ggctccgcgc agcgcgtcga aggtgaggtc gcggatgtcg
     1861 cggccgtcga ggctgatgcc gccggactgg acgtcgtaga agcgcagcag cagcttgatg
     1921 acggtggtct tgccggagcc ggtggggccg acgatggcgg cggtggtgcc ggcggggatg
     1981 tggaacgaca ggtcgcgcag cacgtggggg cggctgtcgg tgtagccttc gtagtcgtgc
     2041 cagctgtagc ggaagctgac gtcgcggaag tcgacttcgc cgcgggcggg cgcgggcagg
     2101 gtggccgtgc cttcgatgac gttggactgg acgtcgagca ggcggaagac gcggccggtg
     2161 gaggccatgg cccgctggta gaggtcgagc gtttcgccga ggcgggtcag cggccagagg
     2221 aggcgctggg tcatgaagac gaggacgctg tagacgccga cgttgagctg gccgttgatg
     2281 accatgacgc cgccgaagac catgatggcg atgaagccgc ccatgatgaa catgcggatg
     2341 aggggcacga aggcggagct gagcttgatg gcggcggcgt tgcgttggcg gtattggtcg
     2401 ctctcggcgg cggcgcggcc gacttcgtac tcctcggcgg tgtagctctt gacggtggcg
     2461 atgccgctga ggttgttgct caggaagctg ttgagaatgc cgacctgttc gcgcacggcg
     2521 gcatagcgcg ggccgaggcg ctgctggaat ttgagcgagc cgtagatgat gaacgggatg
     2581 ggcacgacgg tgagccaggt gaccgacggg gcggcgacga ggaacatgcc cagcacgacg
     2641 acgatggtgg tgacgagctg gatgatctcg ttggcgccga tgtcgaggaa gcgctccagc
     2701 tggttgacgt cgtcattcag gacggacatg aggccgccgg tggattgctc ctcgaagtag
     2761 gccatgtcga ggcgctggac gtggtcgtag gtggacaggc gcaggtcgtg ctggatgtcc
     2821 tgggccaggt tgcgccagcc gacggccttg aggtagtcga acagcgattc gaagccccac
     2881 aggacggcgg tgatgaggcc gacgccgacg agctggtggg tgacgtcgac gacgccgaag
     2941 cgggcgagga tggagtcctg ttgcatgacg acgacgtcga cggccatgcc gatgaggacg
     3001 ggcggggcca ggtcgaagat tttgttgagg atggagtaga gggtggcgag ggcgacgcgg
     3061 ccgcggtagg cgcgggcgta ggtggcgagc cgccgcaggg ggtggatcgg ggtggtggtg
     3121 gttgtcatgg ggagattata gaattacgaa ttacgaatta cgaattacga attaggaatg
     3181 gggaggaatc cacgaaattc acgaaattca cgaattttga aggggagccg cggattttgc
     3241 ctggctgtcc tcagccgcga ttgcgcagat ttgaagggtg gcgttggtgg cattagtggc
     3301 ctttagaaat attgcaaaat atttagaaag tgattatttg tcctgtgggc ttggggggcc
     3361 tgggttgcag gatggtgttt gtggagtttt taaatattta gcaataattc taaaggggtg
     3421 gtggggggga tggatttcac atcaacccta ccggttccag ggcgatcagg tcctcaaatt
     3481 cgttgggccg aaggctctca aattcctttt gcggcacctt gatgtagcgc cagcttgtgc
     3541 cggtcaggtc ggttgcattc cggcaccaca gccgggcggc tccgtccttg tgccgcacgt
     3601 ccacgtcctc ttgccctttc gtttcgatga gatgatggac cccgtcggcc gtgacggcca
     3661 cgaaatccgg ctcgtagtag cgcaggttgg ccacggagtc ggtgtaggtg atgctgaagc
     3721 cgaacgcgcg gggcagcttg ctgaacctga ccacatccgg cgctttttcc agaaaatggc
     3781 cgaaggtcaa ttcaaactga ttgtcggcgg cgagtttgtt gaagatcgtt ttgtcggccg
     3841 cgccgatggg gcgtgaccac ggatagccgg ggcattcgga cagccgccgg ccgttgccgg
     3901 ccagttcggg cgttcgctcc tcgaccacga cctcgcgcag gatggcgaca aaggccttca
     3961 ccgtcacgtg ttgagccacg ggatgggaga tggccttgat catcaccgga tcgtcgagat
     4021 cgaccggctc gccgaaggcg tggagcgcca gaaattcgcg caccttgggt acaagcgccg
     4081 cgaattggga cggcagcttc acgtcggcgg cgatgcgctt ggcgtaatag gagataacct
     4141 cgcccgacgt gcgcacttcg gggatggcat actcgcgctc gatgagcttc tccagcgtga
     4201 tgatgtcgta gccctcatac tggaaggcgt tggcggcggc gtcgccctgt ttccggggca
     4261 aggtcggcgc cttgatgcgg ccgacatcga tggcggcgat ctcctcggcc agcgagcgct
     4321 tgcgctccag cagcgccgac agttcgggga tcgtgatgtc catctcggcc ttggcggggt
     4381 cggggaagat ggtctcgatg accagcttgt ccttgcccgg ctcgaacgac tcgaaggcca
     4441 tctcctcttc tttctcaagc tcttccacga actcgatgaa ggcattgttg ccgatgacgt
     4501 cgacccgctc gcggaagggc ggctccgctt cggtgttgcc gcggaacatg agccgcagac
     4561 cgcggccgat ggtctgttcg ggcaggatgt tggccttggc cgtgtagggg cgcaggccga
     4621 cgacgaccgt caccgattgc acgtcccacc cctcgcgcag catcaggacg ctgacgatgc
     4681 agttgacggg gctgtcgggt ttgtcgatgt cggcggctat ctgccgggcg tcgtcctcat
     4741 cccgcacgga gacgtcgccg accttgttgg tgtggatgac gtgcagccgc tcgccggcga
     4801 actcggccgg gtatttggtg cgcagatagt cggccacgtc gtcggcgtct ttggtgctgt
     4861 tcatcatgac gaacagcacc ggctttctgt tgagcgggac cagttgttcg cggtattcgc
     4921 gccagcgctc gacgccggct gtcagatagg cctggtagcg cacactggcg atgtccgacg
     4981 gcgtttcggc catgccgcga acgacgcctt tatagggtcg cttgacgacg ttatccagaa
     5041 tggcttgctt caacggatag tcgaagactg tccatgtgaa cagcgatccc ttgctgtagc
     5101 gcggcgtggc cgacatatcc agttgaatga c
//
LOCUS       DAOMQG010000246         5111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6873_length_5111_cov_8.199565, whole genome shotgun sequence.
ACCESSION   DAOMQG010000246 DAOMQG010000000
VERSION     DAOMQG010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6873_length_5111_cov_8.199565"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2185)
                     /locus_tag="PLC06_12185"
     CDS             complement(<1..2185)
                     /locus_tag="PLC06_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91250.1"
                     /translation="MSKANVSRRALVEAIVALEAQREAAENDDGLGRAIGVALQALRD
                     RLTYTQAEPADQSRHLLAVLVADLSGYTSLSEHMDVERVRDAMNAMWNVLDGVALAWG
                     GQIDQHAGDSLTALFGLPYSRQGDIRRALHAALTMQQELALFNERARRAAADPSGKSW
                     ADEWPGPSMRIGVHSGPVYFAHSPASFPERSASTTSATTGAAGRATPAGRATPAGRAT
                     LAGRATSVGETITEARRLEGLAPVGQILTSGVVKRQTHGRFFFSPLPATSSEVDGTYL
                     VKAEMPMMTAYVPGKVAGRVTPLVGRAELLDKLQLALQAVIDSQTPHLVTLVGRPGAG
                     KSRLVYEFEKQAGLLSHSPTILRAGTQGVFPDFPYALIRDFLLRRFSIRPQYSSYLIR
                     HCLESGLAEIINRVQESLPLPAWGAEVVSLLERLLDPQAAAAISMDEVQAVFARLLNF
                     MSAHGPVIIVLEGINRADRESLDLVERLVDGGQAGSVLFLGLATATEEVDPQQVLPWL
                     QRGDDLFGSMERLDVPTLSAMDSRLMVAEILGLMTPLPMRLMDLVVAESDGNPLYIEA
                     MVRLLIAREAIILGERRQVDMDQVENAPLPAGLPRLIEAQLANLPQSERKVLQYAAIF
                     GPLCWDTALIELLPDQEMGAAGVEAALLSLEMQDYLAADDAHSFSAAQAYTFRRDSVR
                     DAAYASIPPQERRKLHLNAANWLIANREHARLGVWYSIDDMIARHF"
     gene            2330..>5111
                     /locus_tag="PLC06_12190"
     CDS             2330..>5111
                     /locus_tag="PLC06_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91251.1"
                     /translation="MKLRKLSLQGYKTFASKTEFLFDSGITAIVGPNGSGKSNIADAL
                     RWVLGEQSFGTLRARRSADMIFAGSQQRARAGMAQATITLDNSDGWLPIDYTEVEIAR
                     RSFRSGENEYLINGQQVRLKDVADLLATSGLAERTYTIIGQGLVDQALSLRSDERRAL
                     FEEAAGITHYKTRRAETLRRLEETQRNLQRVHDILEELRPRVTSLKRQATRTRNYEQI
                     SADLRELLRVWYGYKWEQAKSDLRAARSNAASAEHAWTAAREKLLAHQGHFDEAQGRL
                     AQAQLRAGEIQTRRDKLRQEVEAARRSQAIYRERRASFERQIAEADSELSPLETLAAS
                     ARLELEAATADLQAAQIELQAHQTGLQEFRIAFEGRRAEIARAQAEVARVESERRQTQ
                     TTLAQAEGQLTQLRVRLEELTAETDPANAGSDITESVATLESTSTAARNETVRLQAAR
                     NDESRARNADIGRLKELRRALRDAEQRLNKERNDLARQEERVAQLERQGQRAIRLEPE
                     AVVGRLAALIRIPKAHEPAIVAALGERLAAWLVTDGDSLWRAVANARSSSDDSESRLT
                     LAALDAQTQPASRPDTAGESGVIGWADERVDADDAVAPLVRRLLGNILLVKDAPSAYR
                     LAAGWPSGYTAAAPDGVLVHGGGLVEVGAAAGDGILARETRRRDETTRLVDMRAALAK
                     HEAGLVKQQEEIDALQEQVDERANEERRLLRLENEAGQTLNETRRQLENLRQQRDFVA
                     RRQNERATARAQAETRIAQAEMTITSQNERLARSEAALTEARDRLAALPTGEAEQQQQ
                     SLRQSLAAAQTILAGRQAVVDSRRATLNQSEGQLNRLRGRLAGLREQLAGLGREEDEA
                     ETAVLERELAELEVALTPLLSGMEADRALLGRLQADSAGLQRHAHDLETQLTQVRIRQ
                     TQHENQIEGLQER"
BASE COUNT         1036 a         1645 c         1582 g          848 t
ORIGIN      
        1 cgaagtgtct ggcgatcatg tcgtcgatgg aataccagac ccccaatcgg gcatgttccc
       61 gattggcaat cagccagttc gccgcgttta gatggagttt gcggcgctct tgcggcggta
      121 tggaagcata ggccgcgtct cgtaccgaat cacgacggaa tgtataggcc tgcgcagcag
      181 agaagctatg ggcatcatcg gccgccagat aatcctgcat ttcgaggctt aatagcgccg
      241 cctcgacccc ggccgcgccc atttcctgat ccggtagtag ctctatcaag gcagtatccc
      301 agcatagcgg gccgaagata gctgcgtatt gcagaacttt tctctctgat tgcggcagat
      361 tggccaactg cgcttcgata agtcgcggca atcctgccgg caacggcgcg ttttccacct
      421 gatccatatc tacctgccgg cgttcaccaa gtatgattgc ttcccgcgcg atcaataatc
      481 gcaccatcgc ctcaatgtag agtggattgc catcggactc ggctacgacc agatccatca
      541 gacgcatcgg cagcggcgtc ataaggccca ggatttcggc caccataagc cggctgtcca
      601 tggccgacag tgttgggacg tccagccgtt ccatcgaccc aaatagatca tcgccccgct
      661 ggagccaggg caggacttgt tgtgggtcaa cttcctctgt ggcggtcgcc aatcccagga
      721 acaaaacact tcccgcctgt ccgccatcca ccaggcgttc cacgagatcc aacgactccc
      781 gatcggcgcg attgatcccc tctaaaacaa taatgaccgg cccgtgggcg ctcataaagt
      841 ttaataggcg cgcaaagaca gcctgaactt catccatcga gatcgcagcc gccgcttgtg
      901 gatccaatag ccgctccagt aagctaacaa cctcagcgcc ccatgcaggc aggggtaacg
      961 actcttggac tcgattgatg atctccgcca acccgctctc caaacaatgg cgaatgagat
     1021 aggaactgta ttgtggacga atcgagaatc gtcgcagcag aaagtcccgg atcagcgcgt
     1081 aggggaaatc ggggaagacc ccctgcgtcc ccgcacgcag gatcgttggc gaatggctga
     1141 gtaatcccgc ctgcttctcg aactcataaa ccaggcgcga tttgcctgcg ccggggcggc
     1201 cgaccagcgt caccagatga ggggtttggc tgtcgatcac ggcctgcaac gcgagttgca
     1261 gtttgtccag caactcggca cgaccaacga ggggcgtcac acgcccggcc actttgccgg
     1321 gtacataggc ggtcatcatc ggcatttccg ctttcaccag gtaggttcca tcgacttccg
     1381 acgaagttgc tggcagaggg gaaaagaaga agcggccgtg ggtctggcgt ttcacaacac
     1441 ccgatgtgag aatctgcccg acgggggcca atccctccag tcggcgtgcc tcggtgatcg
     1501 tttcgcccac ggacgtggca cgtcctgcaa gcgtggcacg tccggcaggc gtggcacgtc
     1561 cggcaggcgt ggcacgtccg gcagcgcctg ttgtagccga agtggtgctt gccgatcgct
     1621 cggggaatga tgccggtgag tgcgcgaaat aaaccgggcc actatgcacg ccgatgcgca
     1681 tgcttggccc cggccactca tccgcccatg acttgcccga tgggtcggcg gcggcacggc
     1741 gcgcgcgttc gttgaacagc gccaactcct gctgcatggt gagcgcggca tggagcgctc
     1801 gtcgaatgtc accctggcgt gaatagggca gcccaaacaa ggccgtcagg ctgtccccgg
     1861 catgttggtc gatttgcccg ccccaggcca gggccacgcc gtccagcacg ttccacatgg
     1921 cattcatcgc atcgcgcacc cgctcaacgt ccatgtgttc agacagcgag gtatagccgg
     1981 acagatcggc caccagcacg gcgagcaggt gtcgcgactg gtcagccggc tcagcctggg
     2041 tgtaggtcaa cctgtcgcgt agagcctgta gtgccacgcc gatcgcccga cccaggccgt
     2101 catcattttc ggccgcctcg cgctgcgctt cgagtgcgac tatcgcctca accagtgccc
     2161 gacggctaac gtttgccttg ctcacataaa atattatata aatagctgcg tctgcacgcg
     2221 aacctgtacc ggacggcagt gccggcggcc ggcgcaggga cacaagccga ttattaggcg
     2281 acatcgtgcg ccgggtgcgc taaaattccc cagagtcgtt aattaatgta tgaagctgcg
     2341 caaactcagc ctccagggct ataaaacgtt cgcctcgaag acggaatttc tgttcgacag
     2401 cggcatcacg gccatcgtcg gccccaacgg cagcggcaaa agcaacatcg ccgatgcgct
     2461 gcgctgggtg ctgggcgagc aaagctttgg cacgctgcgc gctcgccgtt cggccgatat
     2521 gatctttgcc ggcagccaac agcgcgcccg cgccggcatg gcccaggcga cgatcactct
     2581 ggacaacagt gacggttggt tgcccatcga ctacaccgag gtcgaaattg cccgccgctc
     2641 gttccgctcc ggcgagaacg aatatctcat caacggtcaa caggtgcggc tgaaagacgt
     2701 agccgatttg ctggcgacca gcgggctggc cgagcgcact tacacgatca ttggccaggg
     2761 tctggtcgat caagccctgt ctttgcgctc cgatgagcgc cgcgcgctct tcgaagaagc
     2821 cgcaggcatc acccactaca agacccggcg cgccgaaaca ctccgtcgcc tggaagaaac
     2881 ccagcgcaac ctgcaacgcg tccatgatat cctggaggag ttgcggccac gggtcacgtc
     2941 cctgaaacgg caagccaccc gcacccgtaa ttatgagcag atttcggccg acctgcgcga
     3001 actgctgcgc gtctggtacg gctacaagtg ggagcaggcc aagagcgatc tgcgcgcggc
     3061 gcgcagcaat gccgcttcgg ccgaacatgc ctggacggcc gcgcgcgaga agctgttggc
     3121 ccaccaaggg catttcgacg aagcccaggg ccgtctggcg caagcccagc tgcgggcggg
     3181 cgagattcag actcgccgcg acaagctgcg tcaggaagtg gaagctgccc gccgcagcca
     3241 ggccatctat cgcgagcggc gcgcctcctt cgagcgccag atcgccgaag cggacagcga
     3301 gttatctccg ttggaaacgc tcgcagcgtc ggcgcgcctt gaactggaag cggccacggc
     3361 cgacttgcag gcggcccaga tcgagctaca ggctcatcag accggcttgc aagagtttcg
     3421 catagcattt gagggccggc gggccgagat cgcgcgcgcc caggcagagg ttgcgcgcgt
     3481 cgagtcggag cgacgccaga cccagaccac attagcccag gccgaagggc aactcaccca
     3541 gttgcgcgtg cggctggaag agctgacggc cgagacagac cccgccaacg caggtagcga
     3601 catcacggaa tccgtcgcca cgctggaatc gacttccacc gcagcccgca acgagaccgt
     3661 ccgtctccag gcggcgcgca acgatgaatc tcgcgcccgc aacgcggata tcggccgcct
     3721 gaaagagttg cggcgtgccc tgcgtgatgc cgagcagcgg ctcaacaaag agcgcaacga
     3781 tctggcccgg caggaagagc gcgtcgccca acttgaacgg cagggtcaac gcgccatccg
     3841 attggagccg gaagcggtgg ttggccggct ggccgcgctc attcgcatcc ccaaagccca
     3901 tgaaccggcc atcgtcgccg ctctgggtga gcgtttggcg gcctggctgg tcaccgacgg
     3961 cgacagcctt tggcgcgctg tagccaatgc ccgcagcagc agcgacgaca gcgaatcacg
     4021 cctgacgctg gccgcgctcg acgcgcaaac tcaacctgct tcacggccgg atactgccgg
     4081 tgaatccggc gtcatcggct gggccgacga gcgcgtagac gcggacgacg ccgtagctcc
     4141 tctggttcgg cgactgctgg gcaacatcct gctggtgaag gacgcgccat cggcctatcg
     4201 cctggctgcc ggctggccgt cgggctatac ggctgccgcc ccagacgggg tgctcgttca
     4261 cggcggtggc cttgtcgaag tcggtgcggc cgcaggtgac ggcatcctgg cccgcgaaac
     4321 gcgccgccgc gacgaaacga cccgcctggt cgatatgcgc gccgcgctgg ccaagcacga
     4381 agccggcctg gttaagcagc aggaagagat cgatgccctg caggaacagg tcgatgagcg
     4441 agccaatgag gaacggcggc ttctgcgtct ggagaacgaa gccggtcaaa cgctgaacga
     4501 aacgcgccgg caactggaaa atctgcgcca gcagcgggat tttgtcgcac ggcggcaaaa
     4561 cgaacgcgcc accgctcgcg cgcaggctga gacgcgcatt gcccaggccg agatgaccat
     4621 caccagccaa aacgagcgtc tggcgcgatc agaggcggct ttgactgaag cgcgcgacag
     4681 gttggcggcc ctgcccaccg gcgaagccga gcagcagcag caaagcctgc gtcaatcatt
     4741 ggcggcggcc cagaccattc tggctgggcg gcaggccgtc gtcgacagcc gccgggcaac
     4801 gctcaatcag tccgaaggcc aactcaaccg gctgcgcggg cggctggccg ggttgcgcga
     4861 gcagttggcc gggcttggcc gcgaggaaga cgaagctgaa acggccgttc tcgagcggga
     4921 gttggccgag cttgaggtcg cgctgactcc cttgttgagc ggcatggagg ccgaccgcgc
     4981 gctcctcggc cgcctgcaag ccgactctgc cgggctgcaa cgccatgccc atgacctgga
     5041 aacacaattg actcaagtgc gcatccggca gacgcagcac gaaaaccaga tcgagggcct
     5101 gcaggagcgc a
//
LOCUS       DAOMQG010000247         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6898_length_5091_cov_6.022438, whole genome shotgun sequence.
ACCESSION   DAOMQG010000247 DAOMQG010000000
VERSION     DAOMQG010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6898_length_5091_cov_6.022438"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..518)
                     /locus_tag="PLC06_12195"
     CDS             complement(90..518)
                     /locus_tag="PLC06_12195"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRO91252.1"
                     /translation="MAEKIEIVNEKNENNVKNVTHKVVDFGRDGLYLGLGVVCTVQET
                     VQTLVKRGKEYRGHLVEKGEKFAGENKEKVTELVETPQTVAKDTYKKASESFDKYSEQ
                     VLTRVHIPTADAIDAMTKKVHAVDRKLDKLIKENAAAEKI"
     gene            886..2487
                     /locus_tag="PLC06_12200"
     CDS             886..2487
                     /locus_tag="PLC06_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRO91253.1"
                     /translation="MAAITWPPPAPQSGSAAYPVGSPVTADLWVDPAQGDDGRSGATR
                     DQALATISEAWERIPRGQTLQNTGYRILLVAGDYPEATFPQYWEDRHGTQAFPIILSA
                     ADGPGTARLLSNTMNVFDVEYLYLLGLRLENDGDVFHCEQCDHLLIRQSGFWSANRQA
                     HETIKINQSQYVYIEDSDIGGSEENAIDFVAVQYGHILNNRIHDADDWCIYLKGGSAY
                     FTVAGNEIDHCGTGGFVAGQGTGFEFMVAPWLHYEAYAVKFYNNVIHDTTGAGFGVNG
                     GYDILLAHNTLYRVGSASHGMEFVFGLRGCDGDTGRCAAHRALGGWGPSAAGHEEPVP
                     NRNIYVYNNLLYNPPGFRSEWQHFAIYGPRTPGTGTGIPNPSRTDDNLQIRGNLIWNG
                     PADLPLGIEDDSEGCRPANPTCSEAQLTADNHINQTQPALIDPGGGDFRPAPGSLAGV
                     ITYPLPDFPAWDALTPAVPPGDLANRVPDDRAGQARPAGGGAPGAYLEPGTALGYRLF
                     LPSLVWLNDLRNDPFALSRLQTLVP"
     gene            2619..4946
                     /locus_tag="PLC06_12205"
     CDS             2619..4946
                     /locus_tag="PLC06_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91254.1"
                     /translation="MNRKWRLHSAARWLLLLMFMALLPIVLAAKPAAATNNSPLAAYR
                     PSTLPEIIAAGERHTCALKPDGSVDCWGNNDEGQAVDQAGPYIEVTTGYRHTCALKPD
                     GGVDCGGRNHYGQADDQPGPYSRISAGSDHTCALKQDGSVNCWGRNDFGQATNKSGPY
                     TEISAGGSHTCALTPSGAAECWGNPADGRADNQPGPYTRISAGRSHTCALTTTGAAEC
                     WGSNIVGRAEDQPGPYIQIAAGWGHTCALTPGGAVDCWGDNATGQAADKPGPYIQVSV
                     EYNQTCALRPTGEVDCWGDTSGQATGMPGPYGPWEWPLLAIPEIITTGEEHSCGLTPR
                     GAADCWGGGNYGQAADMPGPYTEVSAGSDHTCALRTNGAVDCWGRNDEGQAADMPGPY
                     TEISAGDNHICALKPNGAADCWGLNDAGQATDMPGPYIRIDAGRSHTCAITASGDVDC
                     WGSDGAGQATDRTGPYMQISAGGAHTCAITPTRRVDCWGDNTNGQSAGQAGLYYQISA
                     SLQHTCGLDSTLQQIDCWGANKWGQSTDQNFGPYSQVSTATAHSCTWTTGNAADCWGD
                     NWGGRADDQPGPFGQYVPWAEVRVRKVIVGDAPGNDWTFSGTLGSFTLPAAGGQKLFP
                     RTPGDVVTISETDRPGWVAAVGCAPGGESGVSSVTLTLNEAVSNVCTFTNTLCLAGTY
                     DDGAACVPADPGHYVAANGATAQAACTPGYYQPNGGQSSCLAADPGYYVPNSGATAQQ
                     QCPAGTTSNGAASACTPITSAGAKVYLPMVIGAKP"
BASE COUNT          945 a         1754 c         1543 g          849 t
ORIGIN      
        1 ttgctacagc ttagcgcaaa ccaaacaaaa aagcccggca caacggccgg gcttttccca
       61 ctatatctta acttgtccag aggcgatgcc tagattttct cggctgccgc gttttccttg
      121 atcagcttgt ccagcttgcg gtcgacggcg tggaccttct tggtcatcgc atcgatggcg
      181 tcggccgtcg ggatgtggac acgggtcaga acctgctcgc tgtacttgtc gaacgattcc
      241 gaagccttct tgtaggtgtc cttggccacg gtctgcgggg tctcgaccag ctcggtgacc
      301 ttttccttgt tctcgcctgc gaacttctcg cccttctcga ccagatgccc gcggtattcc
      361 ttgccgcgct tcacgagcgt ctgcacagtt tcctgcacgg tgcaaaccac acccaggccc
      421 aggtacagcc cgtcacgacc aaaatccacg accttatggg tcacattctt cacgttgttc
      481 tcgttcttct cgttgacgat ttcaatcttc tcagccattt cgttttcctc tcttgaaacc
      541 ggcgtatgat tgggccggta tgaattattg ttgttatctt ttctaacgca ttgcgtcatc
      601 cacatgcata atataacgca tcgcgtcata catgtcaata ggcaatttga aaattggccc
      661 aaagatgaat gaaagctgaa taacacggat tacgcggatt ttcgcagatg ggaataatcc
      721 cttgacgacc cgacgcgccc accgtaacat ggccgaatga tcagaatcgc cacgccacgc
      781 agattcgtat tcaatgccag tgggcttgcc cttctccttt gtctgggatt gctcagctgc
      841 gcgcagacca tcccggaggt gtcggccacg ccggaagagc caaccatggc cgccataacc
      901 tggccgccgc ccgccccgca atccggcagc gccgcctacc ccgtcggctc gcccgtgacg
      961 gccgatctgt gggtcgatcc ggcgcagggc gacgacggcc gcagcggggc cacgcgcgat
     1021 caggcgctgg cgacgatcag cgaagcctgg gagcgcatcc cccgcggcca aaccttgcag
     1081 aataccggct accgcatcct gctggtggcc ggcgactacc ccgaggccac ctttccccaa
     1141 tactgggaag accgccacgg gacgcaggcg ttccccatca tcctgagcgc ggccgacggg
     1201 ccgggcacgg cgcggctgtt gagcaacacg atgaacgtct tcgacgtgga gtatctctat
     1261 ctgctcggcc tgcgcctgga gaacgacggc gacgtcttcc actgcgagca gtgcgatcat
     1321 ctgctcatcc ggcagagcgg cttttggagc gccaaccggc aggcccacga gacgatcaag
     1381 atcaaccagt cgcaatacgt ttacatcgag gacagcgaca tcggcggcag cgaggaaaac
     1441 gccatcgact ttgtggccgt gcaatatggc cacatcctca acaatcgcat ccacgacgcc
     1501 gacgactggt gcatctatct gaagggtggc tcggcctatt tcaccgtggc cggtaacgag
     1561 atcgaccact gcggcacggg cggcttcgtg gccgggcaag ggacgggctt tgagttcatg
     1621 gtcgcgccct ggctccacta cgaggcctac gccgtcaagt tctacaacaa cgtgatccac
     1681 gacaccaccg gcgcgggctt cggcgtcaac ggcggctacg acatcctgct ggcccataac
     1741 accctctacc gcgtgggcag cgccagccac gggatggagt tcgtctttgg gctgcggggc
     1801 tgcgacggcg acacgggccg ctgcgccgcc catcgcgcgc tgggcggctg ggggccgtcg
     1861 gccgcggggc acgaggagcc agtccccaat cgcaacatct atgtctacaa caacctgctc
     1921 tacaacccgc ccggcttccg cagcgagtgg cagcatttcg ccatctatgg cccgcgcacg
     1981 ccggggacgg gcacgggcat ccccaacccc agccgcaccg acgacaattt gcagattcgc
     2041 ggcaatctga tctggaacgg cccggccgat ctgcctctgg gcatcgagga tgacagcgag
     2101 ggctgccgcc cggccaaccc cacctgcagc gaggcccaac tgacggccga caaccacatc
     2161 aaccagacgc agcccgccct gatcgacccc ggcggcggcg acttccggcc cgcgccgggc
     2221 agcctggcgg gagtcatcac ttatcccctg cccgacttcc cggcctggga cgcgctcaca
     2281 ccggccgtcc cgccgggcga tttggccaac cgcgtgcccg acgaccgcgc cggacaagcg
     2341 cggcccgccg gcggtggcgc gcccggcgct tatctggagc cggggacggc tctcggctat
     2401 cggctctttc tgcccagcct ggtgtggctt aatgacttga gaaatgatcc ttttgcgctg
     2461 tcccgtttgc agacacttgt cccataattg tcttcaccta atcgtctata cttctgccca
     2521 ttgcaatggc cggtagcaaa caacaagggc agcttgctcc tgttaccggc ttaccggcca
     2581 catctttgaa ggaataagga gaaatacaat tggagtatgt gaatcggaaa tggcggttgc
     2641 attcggccgc gcggtggttg ctgctgctca tgttcatggc gctcctgccg atagtcctgg
     2701 ccgccaaacc ggcggccgca accaacaata gccccttggc cgcctaccgt ccgtcgaccc
     2761 tgccggaaat catcgctgcg ggcgagcgtc acacctgcgc cctgaagccg gacggatcag
     2821 tcgattgttg gggcaacaat gacgaaggcc aggcggttga ccaggccggc ccctatatcg
     2881 aggtcaccac cggctaccgc cacacctgcg ccctgaaacc ggatggcggg gtcgattgcg
     2941 gggggcgcaa tcattacggc caggcggacg accaacccgg tccatacagc cggatcagcg
     3001 ccggcagcga ccatacctgc gccctgaagc aggacggatc ggtgaactgc tggggtcgaa
     3061 atgactttgg ccaggcgacc aacaagtccg gcccttacac cgagatcagc gccgggggca
     3121 gccacacctg cgccctgacg ccgagcggcg cggccgaatg ctggggcaac cccgccgatg
     3181 gccgcgcgga caaccagccc ggtccgtata cgcgcatcag cgccgggcgc tcccacacct
     3241 gcgccctgac gaccactggc gcggccgaat gctggggcag caatatcgtc gggcgagccg
     3301 aggaccagcc cggcccctac atccagatcg ccgcgggctg gggccatacc tgcgccctga
     3361 cgcccggcgg ggcggtcgat tgctggggcg acaatgccac cggccaggcg gctgacaagc
     3421 ccggccctta catccaggtc agcgtcgagt ataaccaaac ctgcgccctg cggccgaccg
     3481 gcgaggtgga ttgctggggt gacacctccg gccaggcgac cggcatgccc ggcccctatg
     3541 gcccctggga gtggccattg ctggccatcc cggagatcat caccaccggc gaggaacatt
     3601 cctgcggcct gaccccgcgc ggcgcggccg attgctgggg cggcggcaac tatggccagg
     3661 cggccgatat gcccggcccc tatactgagg tcagcgccgg cagcgaccac acctgcgccc
     3721 tgcggacgaa cggcgcggtc gactgctggg gcagaaacga tgaaggccag gcggccgaca
     3781 tgcccggccc ctacaccgag attagcgccg gggacaacca catctgcgcc ctgaagccca
     3841 acggcgcggc cgactgctgg ggtctcaacg acgccggtca ggcgaccgac atgcccggcc
     3901 cctatattcg gatcgacgcc ggccgaagcc acacctgcgc catcaccgcg tccggcgacg
     3961 ttgactgctg gggcagcgat ggcgccggcc aggcgaccga ccgaaccggc ccctacatgc
     4021 agatcagcgc cgggggcgcc cacacctgcg ccatcacccc gacccgccgc gtcgactgct
     4081 ggggcgacaa caccaacggc caatccgccg gccaggccgg cctctactac caaatcagcg
     4141 ccagcctgca gcatacctgc ggtctggata gcaccttgca gcagatcgac tgctggggcg
     4201 ccaacaagtg gggccaatcg accgatcaga acttcggccc ctactctcag gtcagcaccg
     4261 ccaccgccca tagttgcacc tggacaaccg gcaacgcggc cgactgctgg ggcgacaact
     4321 ggggcgggcg ggccgacgac cagcccggcc ccttcggcca atacgttccc tgggcggagg
     4381 tgagggtgag gaaggtgatc gtcggcgacg cgccgggcaa cgactggact ttcagcggca
     4441 cgctgggcag cttcacgctc ccggcagcgg gcggccagaa actgttcccc cgcacgcccg
     4501 gtgacgtggt gacgatcagc gagaccgacc ggccgggctg ggtggcggcg gttggctgcg
     4561 cccccggcgg cgagtcggga gtctcgtcgg tgacgctgac gctcaacgag gcggtcagca
     4621 atgtctgcac tttcaccaac acgctgtgcc tggccggaac ttacgacgac ggcgcggcct
     4681 gcgtgcccgc cgaccccggc cattacgtgg ccgccaacgg tgcgacggcg caggccgcct
     4741 gcacccccgg ctattaccag ccgaacggcg gccagagcag ctgtctggcg gccgatcccg
     4801 gctactacgt gcccaactcc ggcgccacgg cccagcagca atgcccggcc ggcacgacgt
     4861 caaatggagc ggccagcgcc tgcacgccga tcacttcggc cggcgcgaag gtttatctgc
     4921 cgatggtgat tggggccaag ccgtagtcaa aggcgtaggg gcgagacaac cggcggccaa
     4981 cgatccggtc attggacagg gttccgcacg ccggttgtcc cgcccgtact cgtcactcgt
     5041 aattcgtaac tagctatcct acagtttgag aaaagcgttg gtcgtcatac c
//
LOCUS       DAOMQG010000248         5073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6934_length_5073_cov_6.502989, whole genome shotgun sequence.
ACCESSION   DAOMQG010000248 DAOMQG010000000
VERSION     DAOMQG010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_5073_cov_6.502989"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLC06_12210"
     CDS             <1..211
                     /locus_tag="PLC06_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91255.1"
                     /translation="ARHVRGLFRAVFRFLRRKEQTAEFRRERQTIATRWAEFHDYFVD
                     LAATYEKTYALVKRERELATATVKG"
     gene            216..1118
                     /locus_tag="PLC06_12215"
     CDS             216..1118
                     /locus_tag="PLC06_12215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRO91256.1"
                     /translation="MNGPTEPATGEGAWLERAFRVRAYECDPYDHVNHANYLRYMQEA
                     AFDASAAVGYDAARYAAMGRQWLIRETDITYLRPLVYGDTVIVKTWVGDFRRVRSRRM
                     YELRLASSGDMVATASTEWVYLNMETLRPATIPPEMVAAFRHPDLTAGERREPFPEAP
                     PPPPGAFHMRRIVEWRDLDQVRHVNNANYLAYIEECNVAATDAFGWPMDRIMELDVGI
                     VARRYRIEYREPALLHDELEVTSFLSNVRRSTAVRHNEVRRVRDGALLARAWSLWVFV
                     DLKAGRPIRIPPSFMESFGPNVAA"
     gene            complement(1160..3940)
                     /locus_tag="PLC06_12220"
     CDS             complement(1160..3940)
                     /locus_tag="PLC06_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRO91257.1"
                     /translation="MSTEWYKRTAEQTLQEIGTTNEKGLTTAEATSRLEKYGPNELEE
                     KAGRSALEILWEQFANILTVLLILAAVVSMVLGDWVEAVAILVIVVLNGVLGYTQEAR
                     AEQSMAALKKMSVPTVRVRRDGRLQEISARNLVPGDIVVLETGNIIPADGRVTASVNL
                     RVEEAALTGESEPVDKDPSRVFEEDRALGDRRNMVYSGTLVNYGRGEFVVTDTGMATE
                     LGHIANLIQGVEEESTPLQQRLHRLGQVLAWAAIVLVIAVVGLGIWREMASGKTFATI
                     DFPELLLTGVSLAVAAIPEALTAVVTIALSLGAQRMLKRHALIRRLPAVETLGSVTVI
                     CSDKTGTLTLNRMTVQALDMANHSYRFSYSDKTSRLELEPTADNDSTTNPTLDLLLIG
                     GSLNSDATIDEADDTKVIGDPTEAALVSAAALVGMHKAELETAFPRVAEVPFDSVRKR
                     MTTLHSVPESSNGLPDSLVTIWERQTMLDHNNRPDYVAFTKGAIDGLLAISPSVWVDG
                     KIQPLDEAWKKRIMASHDEMAAKGMRVLGVALRPWDSPPDETTEEALEQDLILLGMVG
                     MIDPPRPEVKDAVANCKAAGIRPVMITGDHPLTARHIAQQIGISDKGRFITGQELDHI
                     SPSELEEKIKDISVFARVSPEHKIRLVEVLQGQNNIVAMTGDGVNDAPALKRADIGVA
                     MGITGTDVAKGAAEMVLLDDNFATIVAAVEEGRVIYDNIRRFIKYLLTCNVSEIAVML
                     IAPFLGMPLPLFPLQILWMNLVTDGLPALALGIEPPESDVMKRPPYSATESVFGRGMP
                     TFIVVFGVVLSILALVTGFGLWESGDPAWRTVLFSTLVFAQLGMALSVRSERESLLRT
                     GLLTNKAMLGAIIITVVLQLILIYWGPAQQLFHTEALTTRDLLICFGVGMSVIVLVEI
                     WKVFARARAAKE"
     gene            complement(3977..>5073)
                     /locus_tag="PLC06_12225"
     CDS             complement(3977..>5073)
                     /locus_tag="PLC06_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91258.1"
                     /translation="VYLPLVEAIREQGILRYFPGRTETDFFVWLAHHRAEVEKALGYE
                     IAPDVAVSRLLSRVEEAAEARRTTLADRLRRFARLTITEQPPAMPRRTWAQERALDRY
                     SRHLFAGMVYPVVLSEGAEPPDPNDPAFARAVALCREEEATLCVLGITPDQSAAETAS
                     LEAYRGAFEAAAIHGELLIDSGDPLHLIKEVGFLNDLVIINRSFAAAPTGGLSPSTTT
                     RAIIEQLNRPVLLLGDSVAAPFPRRVLVVHDTRRPMNEAMFIAAYLAERWGCELGVLP
                     LDGGDTQAAIEETIRYLDLHEVTATFMEPIRPGGRAAEQIIQSGIAGDFDLLLMAGPN
                     RDRKTNKNNNSTEPVWGVLEHWPRSTLIAT"
BASE COUNT          860 a         1605 c         1680 g          928 t
ORIGIN      
        1 cgccaggcat gtgcgcggcc tgtttcgcgc tgtcttccgc tttctgcggc gcaaggagca
       61 gacggccgag ttcaggcgcg aacgacagac tatcgccacg cgctgggccg agttccacga
      121 ttactttgtc gatctcgccg ccacgtatga gaagacttac gcgctcgtga agcgggagcg
      181 ggaattggcg actgcgacgg tgaaagggtg aacggatgaa cggcccgacg gaacctgcaa
      241 ccggcgaagg agcctggctg gagcgcgctt tccgcgttcg ggcctacgaa tgcgacccct
      301 acgaccacgt caatcacgcc aactacctgc gctacatgca ggaggcggcg ttcgatgcgt
      361 cggccgccgt cggctacgac gcggcccgct atgcggcgat gggccggcag tggctgatcc
      421 gcgagaccga catcacttat ctgcggccgc tggtctacgg tgatacggtc atcgtcaaga
      481 cctgggtggg cgacttccgc cgtgtccgtt cgcggcgcat gtatgagctg cgcctcgcct
      541 cgtcgggcga catggtcgcc accgcctcga ccgagtgggt ctacctgaac atggaaacgc
      601 tgcgcccggc gacgattcca cctgaaatgg tggccgcgtt ccggcatccc gacctgaccg
      661 ccggcgagcg ccgtgagccg ttccccgaag cgcccccgcc gccgcccggc gctttccaca
      721 tgcgccggat cgtcgaatgg cgcgacctcg atcaggtgcg ccacgtcaat aacgccaact
      781 atctggccta tatcgaggag tgcaatgtgg ccgccaccga cgccttcggc tggccgatgg
      841 accgaatcat ggagctggac gtgggtatcg tcgcccgccg ctatcgcatc gaataccgcg
      901 agccggcgct gctccacgac gaactggaag tgacgtcgtt tctgtccaac gtccggcgtt
      961 ccacggccgt gcgccataac gaagtgcggc gcgtgcgcga cggagcgctg ctggcgcggg
     1021 cctggtcgct gtgggtgttc gtcgatctga aggccgggcg gccgatccgc atcccgccgt
     1081 cattcatgga gagttttggg ccaaatgtgg cggcataaag aagaagggcg acagacagcc
     1141 tgtcgccctt cttctttttt tactccttgg cggcgcgagc gcgggcgaag accttccaaa
     1201 tctcaaccag cacgatgacc gacatgccga cgccgaagca gatcagcagg tcgcgggtgg
     1261 tcagcgcctc ggtgtggaag agctgctgcg ccggccccca gtagatgagg atcagctgca
     1321 ggacgacggt gatgataatc gcccccagca tcgccttgtt ggtcagcagg ccggtgcgca
     1381 gcagcgactc gcgctcggag cgcaccgaga gagccatgcc cagctgggcg aagaccagcg
     1441 tcgagaacag caccgtgcgc caggccgggt cgcctgattc ccacaagccg aagccggtca
     1501 ccagcgccag gatgctcagg acgacgccga agacgacgat gaacgtcggc atgccgcggc
     1561 cgaagacgct ctcggtggcc gaatagggcg gccgcttcat gacgtcgctt tccggcggct
     1621 cgatgcccag cgccagcgcg ggcaggccgt cggtgacgag gttcatccac aggatttgca
     1681 gcgggaacaa gggcagcggc atccccagga acggggcaat gagcatgacg gcgatctcgc
     1741 tgacgttgca ggtgagcagg tatttgatga agcgccggat gttgtcgtag atgacgcggc
     1801 cttcctcgac agcggccacg atggtggcga agttatcgtc gagcagcacc atctcggccg
     1861 cgcccttggc cacgtcggtg ccggtgatgc ccatggccac gccgatgtcg gctcgcttca
     1921 gggcgggcgc gtcgttgacg ccgtcgccgg tcatcgcgac gatgttgttt tgcccctgca
     1981 gcacttcaac cagtctgatc ttgtgctccg gcgagacgcg ggcgaagacg gatatgtcct
     2041 ttatcttctc ttccagctcg gatggggaga tgtgatccag ttcctggccg gtgatgaacc
     2101 ggcccttgtc gctgatgccg atctgctggg cgatgtggcg ggcggtcagc gggtggtcgc
     2161 cggtgatcat gaccgggcgg atgccggcgg ccttgcaatt ggcgacggcg tccttcactt
     2221 ccgggcgggg cgggtcgatc atgccgacca tgcccagcag gatgaggtcc tgctccagcg
     2281 cttcctcggt cgtctcgtcc ggaggcgaat cccacggccg cagggccaca ccgagcacgc
     2341 gcatcccctt ggcggccatt tcgtcgtggg aagccatgat gcgcttcttc cacgcctcat
     2401 ccagcggctg aatcttgcca tccacccaca ccgaggggga gatggccagc agcccgtcga
     2461 tggctccctt ggtgaaggcc acatagtcgg ggcggttgtt atggtccagc atcgtctggc
     2521 gttcccagat ggtgacgaga ctgtcgggga gaccatttga cgactcgggc acgctgtgga
     2581 gcgtggtcat gcgtttgcgc acggagtcga acggcacctc ggccacgcgc gggaatgccg
     2641 tctcaagctc ggccttgtgc atgccgacca gcgcggcggc ggagacgaga gccgcctcgg
     2701 tcggatcgcc gatgactttg gtatcgtctg cctcgtcgat ggtcgcgtcg ctgttcagcg
     2761 agccgccgat gagcagcaga tcgagcgtcg ggttggtggt ggagtcgtta tcggcggtcg
     2821 gctccagctc cagccggctg gttttgtcgc tatagctgaa gcggtaggag tggttggcca
     2881 tatccagcgc ctgcaccgtc atccggttca gcgtcagtgt gccggtcttg tcggagcaga
     2941 tgaccgtgac cgagccgagc gtttccacgg cgggcaggcg gcggatgagc gcgtggcgct
     3001 tgagcatgcg ctgcgcgccc agcgagaggg cgatggtgac gacggccgtc agcgcttcgg
     3061 ggatggcggc cacggccagg ctgacgccgg tcagcagcag ttcggggaag tcgatcgtgg
     3121 cgaacgtttt cccggaggcc atctcgcgcc agattcccag accgacgacg gcaatgacca
     3181 gcacgatggc cgcccaggcc agcacctggc ccagccgatg gaggcgctgc tgcagcggcg
     3241 tggattcttc ttcgacaccc tggatcagat tggcgatatg acccagttcc gtggccatgc
     3301 cggtgtcggt gacgacgaat tcgccgcggc catagttgac cagcgtgccg ctgtagacca
     3361 tgttcctgcg gtcgcccagg gcgcggtctt cttcaaagac gcgcgagggg tccttgtcca
     3421 ccggctccga ttcgcccgtc agcgcggctt cctcgacgcg caggttcacg ctggcggtga
     3481 cgcggccgtc ggcggggatg atgttgccgg tctccaggac cacgatatcg cccggcacca
     3541 ggttacgggc ggagatctct tgcaggcggc cgtcgcgccg cacgcggacg gtgggcaccg
     3601 acatcttctt gagcgcggcc atcgactgct cggcgcgggc ttcctgggtg tagcccagaa
     3661 cgccgttgag gacgacgatg accaggatgg ccaccgcctc aacccagtcg cccagcacca
     3721 tcgacacaac ggcggccagg atcagcagca cggtcaggat gttggcgaac tgctcccaaa
     3781 gaatctccag ggctgatcgc ccggcctttt cctcaagctc gttggggcca tacttttcga
     3841 ggcggcttgt ggcttcggcc gtcgtcaggc ccttctcatt cgtggtacca atctcttgca
     3901 gcgtttgctc cgctgtccgc ttgtaccatt ccgtagacat ctagttcaat tcctcctacc
     3961 tttttgtgac gggtgatcag gtagcgatca aagttgagcg aggccagtgt tccagcactc
     4021 cccagaccgg ttccgtcgaa ttgttatttt tgttcgtttt ccggtcgcga ttcggccctg
     4081 ccatcagcag gagatcgaaa tcgccggcga taccggattg gatgatctgt tccgccgccc
     4141 ggccgcccgg ccggatcggc tccataaagg tggccgtgac ctcgtgaagg tccagatagc
     4201 ggatcgtctc ctcgatggcg gcttgtgtgt caccgccgtc gagcggcagc acacccagtt
     4261 cgcaccccca ccgctcggcc agataggcgg cgatgaacat cgcctcgttc atcggccggc
     4321 gggtatcgtg gacgacgagc actcttcgcg gaaagggagc ggccacagag tcgccgagca
     4381 gcaaaacagg ccgatttaac tgttcaatga tcgctcgcgt cgtggtcgag ggcgacagac
     4441 cgcccgtggg ggcggcggca aacgatcggt taataataac caaatcgttc aaaaaaccca
     4501 cttcttttat caaatgcagg ggatcgccgc tatcgatcag taactcccca tggatcgccg
     4561 cggcttcgaa cgcgcctcta taggcctcaa gggaagctgt ttcggcggcc gattggtcgg
     4621 gggtgatgcc gaggacgcac agcgtcgctt cctcttcccg gcacagcgcc accgcgcggg
     4681 cgaaggccgg gtcgttcggg tcgggcggtt cggcgccctc gctcaggacg accgggtaga
     4741 ccatgcccgc gaaaaggtgc cgggagtatc gatcgagcgc gcgctcctgg gcccaggtgc
     4801 gccgcggcat ggccggcggc tgctcggtga tcgtcagccg cgcaaagcga cgcagccggt
     4861 cggccagggt ggtgcggcgt gcctcggccg cttcctcgac gcgcgacagc agccgcgaga
     4921 cggccacatc gggcgcgatc tcatagccga gcgctttctc cacctcggct cgatggtggg
     4981 ccagccagac gaagaagtcg gtctcggtgc gccccggaaa atagcgcaat atcccttgct
     5041 cgcggatcgc ctcgaccagc ggcagataga cct
//
LOCUS       DAOMQG010000249         5067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6942_length_5067_cov_10.047885, whole genome shotgun sequence.
ACCESSION   DAOMQG010000249 DAOMQG010000000
VERSION     DAOMQG010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6942_length_5067_cov_10.047885"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            733..2100
                     /locus_tag="PLC06_12230"
     CDS             733..2100
                     /locus_tag="PLC06_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRO91259.1"
                     /translation="MTYGQSHCQTVGGCLTPGGDSLPGFPLLALRITNEGTPGSSVID
                     GDSIIRGTKPIFFLMANIHAREITTPEIAMRFIAHLIDGYDVDADATWLVDHREVWVV
                     PTANPDGHQIVELGLKSSYGDFPFYQRKNANNDANRDGNPDCPIWPPLSLSHEQFGID
                     LNRNHSFDWGPPGSSDQPCDMTYRGPSKASESEVYALENLVRTLIPDQRAADLNAPAP
                     KDTVGLLITLHSFSNLVLWPWGSTTLPAPNKNDLKAIGDKFAGFNGYLSCQPTHCLYG
                     TNGATDDWAYGELGIPAFTFEIGNQFMPAYAEIDAVQWPDNRPALIYAAKIARTPYMT
                     VHGPDVLELTVATGIPGPTLHAIIDDRSNGSQPVSAATYSIDIPPDSEGATLRPLEAA
                     DGAFDSITEAVSGKIDLAGIEPGRHILFVRGQDINGNWGAISAIFIDVAVVAEFTLLP
                     TVISP"
     gene            complement(2116..>2352)
                     /locus_tag="PLC06_12235"
     CDS             complement(2116..>2352)
                     /locus_tag="PLC06_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91260.1"
                     /translation="TSHLDSLSEALIQEALQRVMEGRTSLVIAHRLSTILAADLILVM
                     DQGRLVEKGTHGELIEEDGLYASLYETQFRPEHE"
     assembly_gap    2353..2452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2453..3500)
                     /locus_tag="PLC06_12240"
     CDS             complement(<2453..3500)
                     /locus_tag="PLC06_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91261.1"
                     /translation="MGFHGGGWWAFLSHDATQERPVVTRALLLRVWGFARPYIKHVFL
                     LLVTILLTTAITLVSPLLMRSLIDDAIPNGDWRLLNFLALGLIAIPVLNGLVGVWQRD
                     LTAFVGESIIFDLRNAVYEHFQRMSLRFFTQSRTGELMSRLNNDVVGAQQAVSNTLIS
                     IISNIVQVVATLSIMIALEWRLTILGLLVIPFFYLPARRIGRILRDLRRQSMVLNAEM
                     NATMNETLNVSGALLVKLFGREERELARFHRDSAAVRDLGVRSAVIGRWFFMILGIIG
                     AVGTALVYWVGGYLTIQGAFTIGTIVAFGAYLSQLYGPLMALANSPVEFAQSMVSFER
                     VFEVLDIPVEIFDKP"
     gene            complement(3686..>5067)
                     /locus_tag="PLC06_12245"
     CDS             complement(3686..>5067)
                     /locus_tag="PLC06_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HRO91262.1"
                     /translation="NLDGSTKSVSDDTVFPGGIFTYTITVMNSGGTDIPVTLTDELPS
                     EVTYSVHTCPPIITTACGYQNGTVSWEGTAVAGQSVDITVTVVMNMDAVPGTVITNTA
                     QLVSAEQNLEVDADVTVAENVAYLNQLLPIALRPNLGPITLAVGAPNGANTWMLSWTA
                     SYGATGYEIQESNSSNFDGATSIVVGPTTSRAVAKQPSPNNVFYYRARSVIGDVPGPW
                     SNVVTVVGGYRDDFDDPSTGWGLRRGTHLEDVDAFYENGKYVTMITSRWDWLIASPLR
                     PAPRVPYVIEYDARIISQGWVQSAGAVFGGDWGGGECPPNLTGEGWANHSNCFNHFYN
                     INTIFDDTDISNVKMKLIFERVDQVKWDTSGGAPLKRIGDIRDQPGQWGFFKQVNPRD
                     WNHYRIEVRADSIRVYAATVGSPLKLQKEYTDTRWTTSPYFGFFTSTDLIEGATWRFE
                     YIQVMPLDE"
BASE COUNT         1172 a         1443 c         1303 g         1049 t
ORIGIN      
        1 tagcgttacc ttaggcagag tattcacccc cttctttttg tagttttgac atctcggcaa
       61 ttctatcaca ctcgattttg gtcggcaaag ccggaaagaa aatacggaca aacatatggt
      121 gacattggtt ccggcatggc caatggttat aatcctgtca atatcaatct tgtgatctgc
      181 atcatctgga ggtaaatcat tatggatccc gtcggtaccg tcatctttac gatttttatc
      241 gcgctcgtcc ttgcagccat cttcctgcca cggttggggc gcaagaacaa gtaagtcttc
      301 cccccggtgc gccacccgac aaccagacag cttaggctcc tgatagtatt cgtcctgccg
      361 gcactggcat gggcggtcgc tggacgggga catattgcag cgcggccgtt gcgcccgata
      421 gaaagcgaca gccggcctct ggttgtccgc atctactacg acagaataga cgacctggct
      481 gcgcttcaat catacgacgt ttgggaatat aacaacttgc gggaacggta cgtattggcg
      541 gccgtcaatc aagccggcta cgaagcgctc atcgaacagg gttggacggt taccatcgac
      601 ccggatcagc aacgggccat cgacagagca ccctctcaaa gcgcagacta ttacggcggc
      661 tatcgcacca ccgccgagat ttatgccgat ctggctacga tcaattcagc ctccccccaa
      721 ttgacggaac tcgtgacata cggccagagc cattgccaaa cggttggagg ttgcctaacg
      781 cccggcggcg atagtctgcc cggctttccg ctgctggcct tgcgcatcac caatgagggc
      841 acgccgggga gttcggtcat cgacggtgac tcaatcatcc ggggcacaaa gcccatcttc
      901 ttcctcatgg ccaacattca cgccagagag atcaccacgc ctgaaatcgc catgcggttc
      961 atcgcgcacc tgatcgacgg atacgatgtc gatgccgacg caacatggct ggtggatcat
     1021 cgcgaggtct gggttgtgcc cacagctaat cctgacggac atcaaattgt agagttaggc
     1081 ctcaagagca gttacgggga ctttcccttt tatcagcgca agaatgccaa caatgatgcc
     1141 aatcgggacg gcaacccgga ttgcccaatc tggccgccgt tgagcttgag ccacgaacaa
     1201 ttcgggattg atctcaatcg caatcatagc ttcgactggg ggccgccggg ttcgagcgat
     1261 cagccttgcg atatgaccta tcgcgggccg tcgaaagctt ccgagagcga agtttacgct
     1321 ttggagaatt tggtacgaac tctcatcccc gaccagcgcg cggccgactt gaatgctccg
     1381 gccccgaaag atacggttgg gttactgatc acgctccaca gtttcagcaa tctggtgctt
     1441 tggccctggg ggagtaccac actacccgcc ccgaacaaga atgacttaaa ggcaattggc
     1501 gacaagttcg ccggcttcaa cggttatctc tcctgccagc caacccattg cttgtacggg
     1561 acgaacggag ccaccgatga ttgggcctat ggagagctgg gcattccggc gttcaccttc
     1621 gagattggca atcaatttat gccggcctat gccgaaatcg atgccgtcca gtggccggat
     1681 aacaggcctg cgctgatcta tgccgccaaa attgcccgaa caccttacat gacagtccat
     1741 ggcccggacg tgctcgaact cactgttgca acaggcatac ccgggccgac tctccacgcg
     1801 atcatcgacg atcgctctaa cggcagccaa cccgtctcgg ccgcgaccta tagcatcgat
     1861 atccctccgg attcggaagg agccactctc cgacctcttg aggcggcaga tggcgcattc
     1921 gattccatca ccgaagcagt atcaggcaag atcgatcttg cgggcataga acccggaaga
     1981 cacatcctgt ttgttcgtgg ccaggacatc aatgggaact ggggggcaat tagcgcaatt
     2041 ttcattgacg tggcagttgt ggcagagttc acccttctcc cgacggtcat ttctccgtga
     2101 cattaattaa gcaccctatt cgtgttccgg ccgaaattga gtctcataca gagacgcgta
     2161 gagaccgtcc tcctcaataa gctctccgtg agtgcccttc tccaccaggc gaccctgatc
     2221 catgaccaat attaaatcgg ccgccaggat cgtggacagc cgatgggcaa tgaccaggct
     2281 tgtccgacct tccatgacgc gctgcaatgc ttcctgaata agcgcctccg ataatgagtc
     2341 caggtgcgat gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgggcttg
     2461 tcgaagattt ccaccgggat atccagcacc tcaaagacgc gctcaaagct gaccatgctt
     2521 tgcgcaaact caacaggtga gttcgccaga gccataagcg ggccatacag ctgactcaga
     2581 taggcgccaa aagccacaat cgtgcctatc gtgaatgcgc cctggatcgt caaataacca
     2641 cccacccaat agaccagcgc cgtgccgaca gcgccgatga tgcccaatat catgaagaac
     2701 cagcggccga ttaccgcgga tcgcacgccg agatcgcgca cggcagcgct atcgcgatgg
     2761 aatcgggcca gttcacgctc ctcgcggcca aacaacttga ccaaaagtgc gccgctgaca
     2821 ttcagggtct cgttcattgt cgcattcatc tcggcgttga gcaccatcga ttgacggcgc
     2881 agatcgcgca agatacggcc gatgcgtcgc gcgggcagat agaagaaagg gatcacgagc
     2941 aaccccaaaa tcgtaaggcg ccactccagg gcgatcatga tcgacaacgt cgctacgacc
     3001 tgcacgatat tggagatgat cgagatgagc gtattactga ccgcttgctg agcaccaacg
     3061 acgtcgttgt tcagccgcga catcaactcg cccgtgcgcg attgggtgaa aaaccgtaac
     3121 gacatgcgct ggaaatgctc atagacagcg tttctgagat caaagatgat gctctctcca
     3181 acaaacgcgg tgagatcacg ctgccaaacc ccaaccaacc cattgagcac gggaatagcg
     3241 atcaggccca gggcaaggaa attcaaaagc cgccagtcac cgttagggat ggcgtcgtcg
     3301 atgagcgatc tcatcaacaa tggggataca agcgtgatag ccgtggtcaa aagaattgtg
     3361 accaatagca ggaaaacatg tttgatgtat ggacgggcaa agccccagac gcgcagcaac
     3421 agggcacgcg tcacgacagg gcgctcctgc gttgcatcat gacttaagaa cgcccaccaa
     3481 ccgcctccat gaaaacccat tgatcatact cctaatagat tccgatatgg cacagccaaa
     3541 gtacttattc tacgatctaa tcatccaggc cggaacctga tatggccggt tctcatccgc
     3601 atcgacaaca atcgcttaaa caaaaacagc cggcggacat ccatgcaagg tccgccggct
     3661 gcttatccat cactcagagt ttaatctact cgtcgagtgg catgacctgg atgtattcaa
     3721 atcgccaggt tgcaccctcg atgagatccg tcgacgtaaa gaacccaaag tagggactgg
     3781 tcgtccagcg ggtatcggta tattcctttt gcaacttaag gggggaaccc accgtggcgg
     3841 cgtaaacgcg aatactgtcg gcgcgcacct caatccggta atggttccag tcccgggggt
     3901 tgacttgctt gaaaaaaccc cactggccag gctgatctcg aatatcccca atccttttca
     3961 gcggagcgcc gccggatgta tcccatttca cctgatcaac ccgctcgaat atcagtttca
     4021 ttttgacatt gctgatatcg gtgtcgtcaa aaatggtgtt tatgttatag aagtggttga
     4081 agcaattgct gtggttcgcc cagccttcac ctgtgaggtt cggcgggcac tctccgcccc
     4141 cccaatctcc gccaaagact gcgccggccg attggaccca cccttggctg atgatgcgcg
     4201 cgtcgtattc gatgacatag ggcacgcgcg gcgcggggcg cagcgggctg gcgatcaacc
     4261 agtcccaccg gctggtgatc atggtcacat acttgccgtt ctcataaaag gcatccacgt
     4321 cttccagatg cgttccccga cggagccccc agccggtgga cgggtcatcg aaatcatctc
     4381 gatagccgcc aacaaccgtt actacgtttg accatgggcc ggggacatcg ccgatcacac
     4441 tgcgcgcacg atagtagaaa acgttattgg gcgagggctg ttttgccacc gcgcgagatg
     4501 tcgtcgggcc aacgacgatt gacgtcgcgc catcaaagtt gctggaattc gactcctgaa
     4561 tttcatatcc ggttgcaccg taactggcag tccatgacaa catccaggta ttggctccat
     4621 taggcgcacc gacagcgagt gtgattgggc cgagattagg tctcagcgcg atcggcagga
     4681 gctgattaag gtaagcaaca ttctcagcga cggtcacatc ggcgtcgacc tccaaattct
     4741 gttcggcgct gaccaactgc gcggtgttgg tgatgactgt acccggcaca gcgtccatgt
     4801 tcattacaac ggtgaccgtg atatcaacgc tctggcccgc gacggccgtt ccttcccatg
     4861 aaaccgtgcc gttttggtag ccgcatgcgg tggtgataat cggggggcaa gtgtgaacgg
     4921 agtaggtcac ctcgctcggc aactcgtccg tcaacgtcac ggggatatct gtgcccccgc
     4981 tgttcatgac cgtaatcgtg taggtgaata taccgccggg gaagacagta tcatccgaga
     5041 cactcttggt cgaaccgtcc agattgg
//
LOCUS       DAOMQG010000250         5065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6948_length_5065_cov_10.423752, whole genome shotgun sequence.
ACCESSION   DAOMQG010000250 DAOMQG010000000
VERSION     DAOMQG010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_5065_cov_10.423752"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLC06_12250"
     CDS             complement(<1..202)
                     /locus_tag="PLC06_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRO91263.1"
                     /translation="MTPSFTIERIADHVGERVTIEGWLYQSTHKGKLLFLRLRDGTGI
                     TQAVAYRPEVGDELFERLVHLGQ"
     gene            334..540
                     /locus_tag="PLC06_12255"
     CDS             334..540
                     /locus_tag="PLC06_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRO91264.1"
                     /translation="MTIVSISPKYQVVIPKSIREALKLKPGQKVQMIQYKGQIVMIPV
                     RPIESMRGFLKGVDTTIEREADRV"
     gene            537..911
                     /locus_tag="PLC06_12260"
     CDS             537..911
                     /locus_tag="PLC06_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRO91265.1"
                     /translation="MNVVDSSGWLEYFTNSPNAAFFASPIKNQAELLVPVISIYEVFK
                     RVLQQQGEEQALEVAAAMMQGMVTNLDVTTALSAARMSIEMKLPMADSIMLATARAHD
                     AVLWTQDIDFAAIDGVQYVAKQ"
     gene            1010..1282
                     /locus_tag="PLC06_12265"
     CDS             1010..1282
                     /locus_tag="PLC06_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRO91266.1"
                     /translation="MELLRAVAACGSISAAAERMDIPYRTAWQKIHEMETRLGEKLVE
                     TQTGGRHGGGARLTPAAERYVEQFERFAHDMERFVADSFAANFDER"
     gene            1379..2224
                     /locus_tag="PLC06_12270"
     CDS             1379..2224
                     /locus_tag="PLC06_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRO91267.1"
                     /translation="MRKIITLLSILLLLAVAVGCGPKEPEVLRLATTTSTADSGLLDA
                     ILPAFEKANNARVDVVAVGTGQAIELGEAGDADVILVHARAREDAFVAEGHGTQRYDV
                     MYNDFVIVGPTDDPAGVAGMSSAAEALAAIAAAGAPFASRGDDSGTHTKEMSLWEAAG
                     ITPDPTGGWYNSLGQGMGATLTFADEAGAYTMTDRGTFLSMRDNLPNLVVVVGGDSIA
                     DNADPKLLNPYGVIPVNPAKSEAINAELAQKFADWLISPETQKMIGEFGVDKFGQPLF
                     YPNAQ"
     gene            complement(2267..2539)
                     /locus_tag="PLC06_12275"
     CDS             complement(2267..2539)
                     /locus_tag="PLC06_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRO91268.1"
                     /translation="MYIGQVAGTVVATIKHEAFNGRKLLLVERLDLDGTPTADYDICV
                     DAARAGVGDRVLVLDEGNGARQVLGMKTAPIRAVVVGVIDEVALAD"
     gene            complement(2648..3331)
                     /locus_tag="PLC06_12280"
     CDS             complement(2648..3331)
                     /locus_tag="PLC06_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91269.1"
                     /translation="MVLPDYWLTRPSATDDEEARTAFDALLDTTLGGSDCPTIRYTLP
                     WPKWQFLCHVAEHRDIALHGSGDAGIALFEPRQSIDLNEFGNRKAVYAAADGIWPMFF
                     AIVDRDRVDTVSNACVRLIDESGEVYGPYYVFSVSQSALANRPWRTGWVYLLPRQTFS
                     EQPSFAFGSSEVHVPQLAGEVSIRPLAKLMIEPEDFPFLMQIRGHDDQRLQEYATAMQ
                     TAAPWPDDL"
     gene            complement(3379..>3585)
                     /locus_tag="PLC06_12285"
     CDS             complement(3379..>3585)
                     /locus_tag="PLC06_12285"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRO91270.1"
                     /translation="GIKFLIVQPLSKTQQPVGEPVVAADATAQAGPGELVFIVGSREA
                     AQALPETFVPVDHAIVGIVDDVAV"
     assembly_gap    3586..3685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3686..4263)
                     /locus_tag="PLC06_12290"
     CDS             complement(<3686..4263)
                     /locus_tag="PLC06_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HRO91271.1"
                     /translation="MANHPMTAPALALLEFHSIAAGIEAGDAMVKRAPVERIRAGTIQ
                     PGYYLVLVTGEVADVVEAVDAGIIAGGDALRDRLLLPNVHPGVVAALSGARASGDDTT
                     AADALGIVETATVAAGILAADAGLKGAEVQLNQLRLGDGLGGKGLALFVGLVSDVEAA
                     VAIGGDLVADQVVRRAVIARLHGEMWDNVNTGG"
     gene            complement(4263..>5065)
                     /locus_tag="PLC06_12295"
     CDS             complement(4263..>5065)
                     /locus_tag="PLC06_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRO91272.1"
                     /translation="ALMTHKLIRLLVVTGGTPVVRAAMQSGKRAVCAGPGNPPVVVDE
                     TADIEQAGRDIVIGGSFDNNIVCTTEKETFVVASVADPLIRVMEANGAVRLNSWQFNR
                     LWKAVTVSDHGPRKYADMNKAFIGKNAAVLLAEIGISAGPEVRQIVVEVDENHPVVWS
                     EQMMPIMPVVRVANADQAIDLAIEAEHGFRHTAVMHSKNLDNLSRMAREIDCSIFVKN
                     GPCLAGLGYGGEGFCSFTIASPTGEGLTGPISFSRIRRCTLVDSFRIV"
BASE COUNT          973 a         1550 c         1549 g          893 t
ORIGIN      
        1 cctggcccag gtggacgagc cgctcgaaca attcgtcgcc gacttccggc cggtaggcca
       61 cggcctgggt gatgcccgtg ccgtcgcgca ggcgcagaaa gagcagcttg cctttgtggg
      121 tgctctgata gagccaaccc tcgatagtga cgcgctcgcc gacgtggtcg gcgatacgtt
      181 cgatggtgaa ggaaggggtc atataaatct ccgatagata tgcgtcaaag ctggaagtat
      241 agcagaacaa attgagaagg gggacgactt gtcctctgtc gaagggaatg ttacactcct
      301 gtaatacgat tctatcatcg ataggagatt agtatgacga tcgtatccat ttctccaaaa
      361 tatcaggtgg tcatccccaa gtctattcgt gaggcgttga agctcaaacc ggggcagaag
      421 gttcagatga tccagtataa ggggcaaatc gtgatgatcc ccgtccggcc gattgagtcg
      481 atgcgcggct tccttaaggg tgttgatacc accatcgagc gcgaggcaga tcgtgtatga
      541 acgtcgtgga ttcgtcagga tggctggaat acttcaccaa ctcacctaac gcagcgttct
      601 ttgcatcacc gattaaaaac caggccgaac tcctcgtccc ggtgattagc atctatgaag
      661 tcttcaaacg agtgctgcaa caacaaggag aagagcaggc gctggaagtt gccgccgcga
      721 tgatgcaggg aatggtgacc aacctggatg tgacaaccgc cctgagcgcg gccaggatgt
      781 cgattgaaat gaagctgccg atggcggaca gcatcatgct ggcaacggca cgagcgcacg
      841 atgccgtcct gtggacccag gacattgatt ttgccgcgat cgatggcgtc caatacgtcg
      901 ccaagcagta gctgggagcg cgcgacaaaa aagggagtag attaccctgc aaccccacta
      961 caacctctgg ctcgaaatca acggtgaggt cgccctctcg ctatggcgcg tggagcttct
     1021 gcgcgccgtg gctgcttgcg gctccatcag cgccgcggcc gaacgaatgg acatccccta
     1081 ccgcaccgcc tggcaaaaga tccacgagat ggagacccgc ctgggtgaga agctcgtgga
     1141 gacgcagacc ggcggccggc atggcggtgg cgcgcgcctg acccccgcgg ccgagcgcta
     1201 tgtggagcaa ttcgagcgct tcgcccatga catggagcgc ttcgtcgccg actccttcgc
     1261 cgccaacttc gacgagcgct gacctgtttc ctctttttgc ccgccctgtc tcccgcgcta
     1321 tacttcgacc gttatgatca ataatcataa cgctcaaccc tgtacggaga agatctacat
     1381 gcgcaaaata attaccctgc tgtcgattct gttgctgctg gcggtggccg tgggctgcgg
     1441 gccgaaagag ccggaagtgc tgcggctggc gacgaccacc agcacggccg attccggtct
     1501 gctcgacgcc atcctgcccg ccttcgagaa ggcgaataac gcccgcgtcg acgtcgtggc
     1561 tgttggcacg ggccaggcca tcgagctggg cgaggcgggc gacgccgacg tgatcctcgt
     1621 ccatgcccgc gcccgcgagg acgcttttgt ggccgagggg cacgggacgc agcgctacga
     1681 cgtgatgtac aacgacttcg tcattgtcgg gccaaccgat gatccggcgg gcgtggccgg
     1741 aatgagttcg gcggccgagg cactggcggc catcgccgcc gcgggcgcgc ccttcgccag
     1801 ccgcggcgac gacagcggca cgcataccaa ggagatgagc ctgtgggaag cagcgggcat
     1861 cactcccgat ccgaccggcg gctggtataa ctcgctgggg cagggtatgg gggcgacgtt
     1921 gaccttcgcc gacgaagccg gggcctacac gatgaccgat cgcggcacct tcctgtccat
     1981 gcgcgacaat ctgccgaatc tggtcgtggt cgtcggcggc gacagcatcg ccgacaatgc
     2041 cgatccgaag ttgctgaacc cctacggggt catcccggtc aacccggcga agtcggaggc
     2101 gatcaatgcc gagctggcgc agaagttcgc ggattggctc atctcgccgg agacgcagaa
     2161 gatgatcggc gagttcggcg tggacaagtt cggccagccg ctgttctatc ccaacgcgca
     2221 gtagcggact ggggagagtc aggcgatggg gtggtcgcgg cggccgtcaa tcggccaggg
     2281 ccacctcatc gatcaccccc acgaccaccg cgcggatggg ggcggttttc atgccgagga
     2341 cttgccgcgc gccgttgccc tcgtccagca ccagcacgcg atcgccaacg cccgcgcggg
     2401 cggcatcgac gcagatgtcg taatcggccg tgggcgtgcc gtcgaggtcg agacgttcga
     2461 ccagcagcag cttgcggccg ttgaaggctt catgcttgat agtggcgacg acggttccgg
     2521 cgacttgtcc aatatacatg atcgttttta gctctcgtta tagctgcatt ccccgaacgg
     2581 tgctgaagcg actgactacg aacaattgca ggctattgaa gtattactgt aacaaaaaca
     2641 cgatttctca aagatcatcc ggccagggcg cggctgtttg cattgcggtc gcatactcct
     2701 gaagccgttg gtcatcatgc ccgcgaatct gcatcaggaa aggaaaatct tccggctcaa
     2761 tcatcaactt ggccaatggc cggatcgaca cttctccggc gagttggggc acatggactt
     2821 cagaggagcc gaacgcgaag gacggttgct ccgagaaggt ttgtcgcggc aggagataga
     2881 cccaaccggt tcgccagggc cggttcgcca gggccgattg gctgactgaa aacacataat
     2941 aggggccata aacctcgcca gactcgtcga tcaatcgaac gcaggcattg ctgaccgtat
     3001 ccacccggtc gcgatcgaca atggcgaaga acatgggcca tatcccgtcg gcggcggcat
     3061 agacggcttt ccggttgccg aattcattga ggtcaatcga ctgccgtggc tcgaacaggg
     3121 cgataccggc gtcgccggaa ccatgcaggg cgatgtctcg atgctcggcc acatgacaca
     3181 gaaattgcca tttgggccag ggcaatgtgt accgaatagt cgggcaatcg ctgccgccga
     3241 gcgtcgtgtc gagtaacgca tcgaaggccg tccgcgcttc ttcgtcatcg gtcgcactgg
     3301 ggcgggtcag ccaataatcc ggcagcacca tagaggtcat tcctgtgggt gggtaacggt
     3361 tggcgatatc gtcaggcgct aaacagccac gtcatccaca atgcccacaa ttgcgtgatc
     3421 gaccggcaca aacgtctccg gcagggcctg ggccgcctcg cggctgccga cgatgaacac
     3481 cagctcgccc ggcccggcct gcgccgtggc gtcggcggcc accaccggct cgcccaccgg
     3541 ctgctgcgtc ttgctcagcg gctggacgat gaggaacttg atcccnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnccgcc ggtgttgaca ttatcccaca tctcgccgtg
     3721 gagccgggcg atgaccgccc gccgcaccac ctgatcggcg acgagatcgc cgccgatggc
     3781 cacggccgct tccacatccg acaccagccc gacaaagagc gccaacccct tgccgcccag
     3841 cccgtcgccg agccgcagct ggttgagctg cacctcggcc cctttcagcc cggcgtcggc
     3901 ggccagaatg cccgcggcca cggtcgccgt ctccacgata cccagcgcgt cggccgcggt
     3961 tgtatcgtct ccggaagcgc gcgcgccact caacgccgcc accacgcccg gatggacgtt
     4021 gggcaacagc agccggtcgc gcagcgcgtc gccgccggct atgatgcccg cgtctaccgc
     4081 ttcgaccaca tcggcaacct cgcccgtcac cagcaccaga tagtaacctg gctggatcgt
     4141 cccggcccgg atgcgctcca ccggggcgcg tttcaccatg gcgtccccgg cctcaatgcc
     4201 ggcggcgata gagtggaatt caagcagggc gagcgcggga gcggtcatcg gatgattggc
     4261 catcagacaa tgcggaatga atcgaccagc gtgcagcggc ggatgcggct gaagctgatc
     4321 gggccggtca ggccctcgcc ggtcgggctg gcgatggtga acgagcagaa gccctcgccg
     4381 ccatagccca gcccggccag acaggggccg tttttgacaa aaatactgca atcgatctcc
     4441 cgcgccatgc ggctcaggtt gtcgagattt ttggaatgca tcacggccgt gtggcggaag
     4501 ccatgttcgg cctcgatggc caggtcgatc gcctgatcgg cgttggccac gcgcaccacg
     4561 ggcatgatcg gcatcatctg ctccgaccag acgacggggt gattctcgtc cacctcgacg
     4621 acgatctgcc gcacttccgg cccggcacta atgccgatct cggccagcag cacggcggcg
     4681 ttcttgccga tgaacgcctt gttcatgtcg gcgtacttgc gcgggccgtg gtcggagacg
     4741 gtcaccgcct tccacaggcg attgaactgc cacgagttga gccgcaccgc gccgttggct
     4801 tccatgacgc gaatcagcgg gtcggccacc gaggcgacga cgaacgtctc cttctcggtg
     4861 gtgcagacga tgttgttatc gaatgagccg ccgatgacga tatcgcgccc ggcctgctcg
     4921 atgtcggccg tttcgtcgac gacgaccggc gggttgcccg gcccggcgca aacggcgcgc
     4981 ttgccgctct gcatggccgc acgaacgaca ggcgtgccgc cggtgacgac cagcaggcgg
     5041 atgagcttgt gggtcatcag cgcct
//
LOCUS       DAOMQG010000251         5039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6984_length_5039_cov_5.333066, whole genome shotgun sequence.
ACCESSION   DAOMQG010000251 DAOMQG010000000
VERSION     DAOMQG010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6984_length_5039_cov_5.333066"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLC06_12300"
     CDS             <1..678
                     /locus_tag="PLC06_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91273.1"
                     /translation="SAAGLAPTGKWKHNINAFWIPDASADDVLISFTQNRRPVPGLVD
                     PVDGMDLVYWDGSTFSFWFDGSDVGLTNKTQEKIDGLHVLPGSQSPIGSGCLSYLLIS
                     TQGPGKVPNYSGGQLKFQGEDVVGFCATSLGENTTGLWHMVLDGSVEGMPRNATDSIS
                     VSADGNTLYLTTQRTFNVDSASGGHSMVYAYDFGTGTFSGPYFDAPANGLPEKVDGLQ
                     VEGELGN"
     gene            1095..4202
                     /locus_tag="PLC06_12305"
     CDS             1095..4202
                     /locus_tag="PLC06_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91274.1"
                     /translation="MEYTNRSRSHSAARWLLLILLLLLLPLALAVRPAVAANIIPAGP
                     TTIVNAGGTLCALMPSGAARCWGQGSVVGVQPGPYVALAAGEFHACALTPAGAADCWG
                     GGGAGGGVIDQPGPYIQISAGWGHTCALTPTGAVDCWGDNSDGESENQPGPYTQISLG
                     SDHTCALKPDGSVDCWGDNANGQATDMPGPYTQISAGLWQTCGLTPAGAVDCWGYYGS
                     SGSTATGNPGPFIQVSAGASGACGLTAAGAADCWGLGITDAGDRPGPFTQLAAGNFES
                     CGLTPDGAVTCWEGSMLWLDEAGPYGPYRPPALPEIVAAGELHTCALTPDGAVDCWGD
                     NSEGQSVDQLGPFIEVTTGYRHTCALAPDGSVDCWGRNNYGQATDKPGPYTQISGGSD
                     HTCGLTPAGAVECWGKNDNGQLNAQPGPYVQVSAGSAHTCALRISGAADCWGHTGDGR
                     SDDKVGPYVQVSAGRSHTCALEPVGNADCWGLNTAGRADDQPGPYVQIAAGWPNTCAV
                     TTSGAVDCWGDNASGSSTDQPGPYVQVTTEGAHTCALRPNGAVDCWGGNANGQAEDMP
                     GPYGLYVSRTPEFIGTGEHHSCALTPRGAVDCWGGGEFGQAADMPGPYTQASVGRFHT
                     CALTTTGAVDCWGLNDDGQATDMPGPYTQVSAGSGQTCALRPDGSADCWGKNDVGQAT
                     DQPGPYVRIDAGWTHTCALTPVGAVDCWGDNAAGQSADMPGPYTQVSAGWTHTCALTL
                     DGRADCWGQNGHGQATDQAGPYSQIGAGTYHTCGVVAGAAGGTGIDCWGNNQFGQGVD
                     QTGNYTQVSASLAHSCALTATGAADCWGENFNGRADDQPGPYGPYVPWAEVTVKKVVL
                     GDAPGDDWVFGGTLGSFTLPAAGGENLFARTPGDVATISETDRPGWVASVGCAPGGES
                     GAASVTLTLNEAVSNVCTFTNTLCLAGTYDDGAACVPADPGHYVATSGSTAQTACTPG
                     YYQPNGGQSSCLAADPGYFVPGSGATAQQQCPAGTTSGPAAGACTPITSTGAKVYLPM
                     VIGGK"
     gene            4470..4721
                     /locus_tag="PLC06_12310"
     CDS             4470..4721
                     /locus_tag="PLC06_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91275.1"
                     /translation="MLKSVEGIYRDGKIELIETPANMDEDTPVIVTFLTGQYVDLRAG
                     GISRKQALVLRERLSGFAEDWDSPEMELYDRYDTIKSSD"
     gene            complement(4814..>5039)
                     /locus_tag="PLC06_12315"
     CDS             complement(4814..>5039)
                     /locus_tag="PLC06_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91276.1"
                     /translation="PHPRRPGQSADAAAADRRLSSAKPGVITNYELRMKRVWGQWISI
                     QWISIQWISIQWISIQWVSIQWISIQWVSG"
BASE COUNT          898 a         1715 c         1595 g          831 t
ORIGIN      
        1 tcggccgccg gtctggcccc caccggcaag tggaaacaca acatcaacgc cttctggatc
       61 cccgatgcgt cagccgacga cgtgctcatc tccttcaccc agaaccgccg ccccgtgccc
      121 ggcctcgtcg acccggttga cggcatggac ctggtctatt gggatggctc gaccttctcc
      181 ttctggttcg atggctccga cgtgggtctg accaacaaga cccaggagaa gatcgacgga
      241 ctgcatgtgc tgccgggcag ccaatcgccc atcgggagtg gctgtctctc ctatttgctc
      301 atcagcacac aggggccggg caaggtaccg aactactccg gcggccagct caagttccag
      361 ggtgaggatg tggttgggtt ctgtgccaca tcactgggtg agaacacgac cggcttgtgg
      421 catatggtgc tcgatggcag tgttgaaggg atgccgcgca acgcgacaga ctcgatctcg
      481 gtcagcgccg acggcaacac cctctatctg accacgcagc gcacgttcaa cgtcgacagc
      541 gcgtcgggcg gccactcgat ggtctacgct tacgacttcg gcacgggcac cttcagcggc
      601 ccatacttcg atgctccggc caacggcctg cctgagaagg tcgacgggct gcaggtggaa
      661 ggcgagctgg ggaattagga attacgaatt acgaattacg aattacgaat tacgaatgat
      721 gtagcacaat ctgccggctt gtagcgcaat ctgtcagatt gcgattgccg gcaacccgac
      781 gtagcgcaat ctgccggatt gcgagaagaa aagaggccat caggcgggtt gagtcatctc
      841 aacccgcctg atttgatcca tccctgaccg acatccgcac cggccgccgg cgcgcccggc
      901 cgatccgtcc ggtgaaattc acaccaatct gtggattttt ccccattttg tgtcgtctac
      961 ctgacagaca gccgccctgc aaatggtcta tactactgtt ccgatcgcga acaggagcac
     1021 ggacacaggc ggagcgtctt gcttccgccg ggcggcttcc tggttatggc acgcgatcga
     1081 cgaggagaat aataatggaa tatacgaatc gatcccggtc tcattcggcg gcgcgttggc
     1141 tgctgctgat cctgttactg ttgcttttgc ctctggccct ggccgtccgg ccggcggtcg
     1201 cggccaatat cattcccgcc gggccgacga cgattgtcaa tgccggcggc acgctctgcg
     1261 ccctgatgcc gtccggggcc gcgcggtgct ggggccaggg cagcgtcgtg ggcgtccagc
     1321 ccggccccta cgtcgcgctc gccgcgggcg agttccacgc ctgcgcgctg acccccgccg
     1381 gcgcggccga ctgttggggc ggtggcggcg ccggcggcgg ggtgatcgac cagcccggcc
     1441 cctacatcca gatcagcgcc ggctggggcc acacctgcgc gctgacgccg accggcgcgg
     1501 tcgattgctg gggcgacaat agcgacgggg agtcggaaaa ccagcccggc ccctacaccc
     1561 agatcagcct cggttctgac cacacctgcg cgctgaagcc cgacggctcg gtcgattgct
     1621 ggggcgacaa cgccaacggc caggcgacgg acatgcccgg cccctatacc cagatcagcg
     1681 ccggcctctg gcagacctgc gggctgacgc cggccggcgc ggtcgactgt tggggatatt
     1741 acggcagcag cggcagcacg gcgaccggca accccggccc ctttatccag gtcagcgccg
     1801 gcgcttccgg tgcctgcggg ctgacggcgg ccggcgcggc cgactgctgg ggtctgggta
     1861 tcaccgacgc gggggaccgg cccggcccct tcacccagtt ggccgccggc aactttgaat
     1921 cctgcggttt gacccccgac ggcgcggtca cctgctggga aggttccatg ttgtggttgg
     1981 acgaagccgg cccctacggc ccctaccgcc cgccggccct gccggagatc gtcgccgcgg
     2041 gtgaactcca cacttgcgcc ctgacgcccg acggcgcggt cgattgctgg ggcgacaaca
     2101 gcgaaggcca gtcggttgac cagctcggcc ccttcatcga agtgaccacc ggctaccgcc
     2161 acacctgcgc cctggcgcct gacggcagtg tcgattgctg ggggcgcaac aactatggcc
     2221 aggcgaccga caagcccggc ccctatacgc agatcagcgg cggcagcgac cacacctgcg
     2281 ggctgacccc ggccggggcg gtcgaatgct ggggcaagaa cgacaacggc cagttgaacg
     2341 cccagcccgg cccctatgtg caggtcagcg ccggcagcgc ccacacctgc gccctgagga
     2401 tatccggcgc ggccgactgc tggggccaca ccggcgacgg ccgctcggac gacaaggtcg
     2461 gcccctacgt ccaggtcagc gccgggcgtt cccacacctg tgccctggag cctgtcggca
     2521 atgccgactg ctggggcctc aacaccgccg gtcgcgccga cgaccagccc ggcccctacg
     2581 tccagatcgc cgccggctgg cccaacacct gtgcggtaac gacatccggc gcggtcgatt
     2641 gctggggcga taatgcctcc ggttcatcaa ccgaccagcc cggcccctac gtgcaggtga
     2701 ccaccgaggg cgcccacacc tgcgctctgc ggcccaacgg cgcggtcgat tgctggggcg
     2761 gcaacgccaa cgggcaggcg gaagatatgc ccggccccta tggcctctat gtgtcgcgga
     2821 cgccggagtt tatcggcacg ggcgagcacc acagttgcgc cctgaccccg cgcggcgcgg
     2881 tcgattgctg gggcggcggc gaattcggcc aggcggccga catgcccggc ccctataccc
     2941 aggccagcgt cgggcgcttc cacacctgcg ccctgacgac gaccggcgcg gtcgactgct
     3001 ggggcctcaa cgacgacggc caggcgaccg acatgcccgg cccctatacc caggtcagcg
     3061 ccggttccgg tcagacctgc gccctgcggc ccgacggctc ggctgattgc tggggcaaga
     3121 atgacgtcgg gcaggcgacc gaccagcccg gcccctacgt ccggatcgac gccgggtgga
     3181 cccacacctg cgccctgacc ccggtcggcg cggttgattg ctggggcgac aatgccgccg
     3241 gccagtcggc cgacatgccc ggcccctata cccaggtcag cgccgggtgg acccacacct
     3301 gcgccctgac cctcgatggg cgggcggatt gctggggcca aaacggccat ggccaggcga
     3361 ccgaccaggc cggcccctac tcgcagatcg gcgccggcac ataccatacc tgtggggtag
     3421 tcgccggggc ggcgggggga accgggatcg attgctgggg caacaaccaa ttcggccagg
     3481 gagtcgacca gaccggcaat tacacgcagg tcagcgccag cctcgcccac agttgcgccc
     3541 tcacggcgac cggcgcggcc gactgctggg gcgaaaactt caacggccgg gccgatgacc
     3601 agcccggccc ctatggcccc tacgtgccct gggccgaagt gacggtgaag aaggtcgtac
     3661 tgggcgacgc gccgggcgac gactgggtct tcggcggcac gctgggcagc ttcacgctcc
     3721 cggcggcggg cggcgagaat cttttcgccc gcacccccgg tgatgtggcg accatcagcg
     3781 agaccgaccg gccgggttgg gtggcgtcgg ttggctgcgc ccccggcggc gagtcgggag
     3841 cggcttcggt gacgctgacg ctcaacgagg cagtcagcaa tgtctgcact ttcaccaaca
     3901 cgctgtgtct ggccggaacc tatgatgacg gcgcggcctg tgtacccgcc gaccccggcc
     3961 attacgtggc gaccagcggc tccacggcgc agacggcctg cacgcccggc tactaccagc
     4021 cgaacggcgg ccaaagcagc tgcctggcgg ccgatcccgg ctacttcgtg cccggctccg
     4081 gcgcgacggc ccagcagcaa tgcccggcag gcacgacgtc cggcccggcg gccggcgcct
     4141 gcacgccgat cacctcgacc ggcgcgaagg tctatctgcc gatggtgatt ggcgggaagt
     4201 aggaagcaga gagcgggcag tagatcagta ttcagtaggt cagtagatca gtattcggca
     4261 gatcggtatt caagcccaaa ctgacccact gacctactga cctactgacc cactgaccca
     4321 ctgacccact gacccactga tctactgacc cactgaccca ctgacttaca ggcagagtat
     4381 tcgttgtccg tggtctcatc agcggcctat cgcccgtcgt tgtgctatac ttggcttgct
     4441 ccggaaaggc ctcatgagga gagttggaaa tgctaaagag cgtggaaggc atctataggg
     4501 acggcaagat agagttaatc gaaaccccgg cgaacatgga cgaggataca cctgtgatcg
     4561 ttaccttcct gaccggccaa tacgtcgatc ttcgagccgg cggcatcagc cgcaagcagg
     4621 cgttggtatt gcgggaacgc ctgagcggtt ttgccgagga ttgggatagc ccggagatgg
     4681 aactctatga tcgatacgat acgatcaagt cttccgacta aacgtggcga tgtcgtcctc
     4741 gtgctatacc cgaactcaaa cacaccttgg gcctctagct caggctctcc ctcaaggtag
     4801 cgaccgcaga tcgctaacca ctgacccact gaatactgat ccactgaata ctgacccact
     4861 gaatactgat ccactgaata ctgatccact gaatactgat ccactgaata ctgatccact
     4921 gcccccacac cctcttcatt cgtaattcgt aattcgtaat tacacctggt tttgcgctcg
     4981 atagtcggcg atctgctgcc gcagcgtctg ccgattggcc agggcggcgc gggtggggg
//
LOCUS       DAOMQG010000252         5035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_6991_length_5035_cov_9.577711, whole genome shotgun sequence.
ACCESSION   DAOMQG010000252 DAOMQG010000000
VERSION     DAOMQG010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6991_length_5035_cov_9.577711"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..920)
                     /locus_tag="PLC06_12320"
     CDS             complement(135..920)
                     /locus_tag="PLC06_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91277.1"
                     /translation="MARNRVVVTIALPLLAAILIGVISGSFFAPKADAGNAAPVFAAL
                     GITAWFIGLRLYGLRGMGLRGGRPLFAGIGFAVLAWVAVLIGRFLPMWPQATIDETGQ
                     AIVEIALHVQVMVIQSAGAGRAFLYLLLFEAFAVQLWAFGVVFRALAEWRGGLTAAIA
                     AGILFGATGFVFFRESFVAGLPALAYFLSWGILYGIIRLRTGSIIGPIIVQALQSFTA
                     WFVFEPPADIAQTGIRTVYLAISVLFAVIIWRLWPRLESDYRV"
     gene            complement(1192..1725)
                     /locus_tag="PLC06_12325"
     CDS             complement(1192..1725)
                     /locus_tag="PLC06_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRO91278.1"
                     /translation="MKIGLFYGSNTGNTELDAEMVKNTFESFAPVTVDLFNIGSVDLA
                     EMEKYDRLIIGCPTWNIGQLQDDWDLKFDQLDKLDLHGKRVALFAPGDQYGYPDNYCD
                     AIGIIGKKMESRGAELVGFTDASDYQFENSLAVEDGVFLGLALDDDNEAELTEPRIRE
                     WVLQLMVDFEVNETVGA"
     gene            complement(1936..2322)
                     /locus_tag="PLC06_12330"
     CDS             complement(1936..2322)
                     /locus_tag="PLC06_12330"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRO91279.1"
                     /translation="MRWVMRSKIHKATVIQADVNYVGSITIDEALMERVGLWAGERVL
                     VVSNTSGARLETYVIPGPRDSGVINMNGAAAHLVKAGEEIIIIAFELSGVPVTPKQIL
                     VDKQNRYVRDLEWVESFSMLESDLTF"
     gene            complement(2377..3540)
                     /locus_tag="PLC06_12335"
     CDS             complement(2377..3540)
                     /locus_tag="PLC06_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRO91280.1"
                     /translation="MPRIREIITYESTLPTGARNAITDVAGVTVGHFTLVSGESDTAP
                     DWRPGNGPFRTGVTIILPHDGKLYEEKVAAAVHTVNGFGKVAGFEQVRELGNLETPIA
                     LTGTMNVPRVADALMTIMAEQSPHIGVGFPETGWSGYSSVNPVVGETSDGYLSDLQAR
                     PIGLAEVRAALAAASAEVAEGAVGAGAGTSCYGWKGGIGTASRVLPAEMGGYTVGALV
                     QTNFGRAEELTICGVPVGRRLRPPVSDRKGEREKGRKGAREGQTVDLGGSIMMVLATD
                     APLDARGLGRLCRRAAFGLARTGSTCHGGSGDFVIAFSTTNRIADRPPMPPEPRPVVD
                     EQSIVSWLSVAVIESIEEAIYNSLLMAGTVTGRNGHVRHGLPADEVARLVGGR"
     gene            complement(3664..4530)
                     /locus_tag="PLC06_12340"
     CDS             complement(3664..4530)
                     /locus_tag="PLC06_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HRO91281.1"
                     /translation="MTQHYGGQSMVAPLQTVLVRRPDAAFGAADPLLWHYVDQPYLPL
                     AQQEHDAFVNTLLGRGVEVVYHDAPLPDHADAIFVHDPVLMTDRGAVILRMGKYLREG
                     EEDAIATSLQRLGVPIHYRLSGTATAEGGDLLWLDERTLCVGLGFRTNQAGFDQLGEA
                     LAGSGVTLVPVQLPYFEGPEACLHLMSLISLVDQDLAVIYRSLLPVPFYQLLVARGVE
                     LIDVSDEEFATMGTNVLALGPRDCVMLEQNHETRAALEAAGCRVATYRGDEMSLKAEG
                     GATCLTRPILRG"
     gene            complement(4569..>5035)
                     /gene="rpiA"
                     /locus_tag="PLC06_12345"
     CDS             complement(4569..>5035)
                     /gene="rpiA"
                     /locus_tag="PLC06_12345"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HRO91282.1"
                     /translation="LDDYPSVDVTIDGADEIDPQLNLIKGLGGALLREKIVAAASRRL
                     IIIADDSKRVEQLATRAPVPVEVIQFARRPVADYLASLGARVVERRKDGERFITDEGN
                     LILDCHFPGLPNPSEIAQLIRAQPGVVEHGLFLGMATEAIVAGERGILVLER"
BASE COUNT          949 a         1679 c         1494 g          913 t
ORIGIN      
        1 acgacgccgg cgcgcccctc gatgcgggtc agaatgttct cgtaggtcat catactctcc
       61 cgtgaatgat ggccgcggac aggaacaggt cgttaagttc ccccgctccg tcgaaaccca
      121 cggccgttga ttttctacac acgataatcc gattcgagtc gaggccacag ccgccagatg
      181 atgacggcaa acaggacgct aatcgccaga tagaccgtcc ggatgcccgt ctgcgcgatg
      241 tcggcgggtg gctcaaaaac gaaccaggcg gtgaagctct gcagagcctg gacgataatc
      301 gggccgatga tgctgccggt gcgcaggcga atgatgccgt agaggattcc ccagctcaga
      361 aaataggcca gagccggcaa cccggccacg aacgattcac gaaaaaagac gaagcccgtc
      421 gccccgaaga gaatgccggc ggcgatggcc gcggtgagtc cgccgcgcca ttcggccagc
      481 gctcgaaaga cgacgccgaa cgcccatagt tgcacggcga atgcctcgaa gagcaacaga
      541 taaaggaagg cccgccccgc gcccgccgac tggataacca tgacctggac atggagagcg
      601 atctccacga tagcctggcc ggtttcgtcg atggtggcct gcggccacat gggcaggaag
      661 cggccgatga gcaccgccac ccaggccagc acggcgaacc ctatcccggc aaagagcggc
      721 cggccgccgc gcaagcccat cccgcgcagc ccgtagaggc gcaagccgat gaaccaggcg
      781 gtgatgccca gcgcggcgaa gacgggcgcg gcgttgcccg cgtcggcctt gggcgcaaaa
      841 aaagagccgc tgatgacgcc gatcagtatg gcggccagca gcggcagggc gatagtgacg
      901 acgacccggt tgcgggccat ctcgggtgat atctctgcgg gcggcggcgc attgtccgcc
      961 cccgtacttt tagatggcct gttaggtggc ctattggatg ccataggccg gattgtagca
     1021 ccccgcggcc gattgtcacc cggagcggag cagacttcct acataaacgt cgaacgcaga
     1081 tgacaccgat ccggcgaatg cgcatcgatc aaaataacgc cttgatccgc gcgaattcgc
     1141 aaaaccggcg tcatccgcgt tctattcccc cacaaagacg acttgccgct ctcaggcgcc
     1201 aaccgtctca ttcacctcaa aatcgaccat caactgcaac acccactccc ggatgcgcgg
     1261 ctcggtcagt tccgcctcgt tgtcgtcgtc gagcgccagc cccaggaaca cgccgtcctc
     1321 aacggccagc gagttttcga actggtaatc gcttgcatcg gtaaagccca ccagctcggc
     1381 gccgcgcgac tccatcttct tgccgatgat gccgatggcg tcgcaatagt tgtcggggta
     1441 gccgtattgg tcgccggggg cgaacagcgc cacccgcttg ccgtgcaggt cgagcttgtc
     1501 gagctggtcg aacttcagat cccagtcgtc ctgcaactgg ccgatattcc acgtggggca
     1561 gccgatgatc agccgatcat acttttccat ctcggccaaa tccaccgacc cgatattgaa
     1621 cagatcaacc gtcaccggag caaaagactc gaacgtattc ttgaccatct ccgcgtccag
     1681 ttcggtgttg cctgtgtttg atccgtaaaa gagtcctatc ttcatggttg tcattctccc
     1741 gccgtcgggc gcgggctgta tctgtttctt ttctatgccc tgtcgcgcct cggtgtataa
     1801 ccacatagta gacgatatgg gccgtcgtgt aattgatcca cgtcaacaag aaaaacaaaa
     1861 agctaccccg cctcatgccg gagtagctct gtggggggaa ccgccgtggt tcccgatttc
     1921 ttttgattcg accgattaga acgtcaggtc ggactccagc atggagaagg attccaccca
     1981 ctccaggtcg cggacgtagc ggttttgctt gtccacgagg atctgttttg gcgtgaccgg
     2041 cacgccgctc aattcgaaag cgatgatgat gatctcttca ccggccttga ccaggtgcgc
     2101 ggccgcgccg ttcatattga tgacgcccga gtcgcgcggg ccggggatga cgtaagtctc
     2161 caggcgcgcc ccgctggtgt tgctgaccac cagcacgcgc tcgcccgccc agaggcccac
     2221 gcgctccatg agcgcctcat cgatcgtgat actgccgaca taattgacgt cggcctgtat
     2281 gaccgtcgcc ttgtgaatct ttgaacgcat aacccatctc atacaatcta ttgtacgtga
     2341 gtttgcgtat gagaataata agaatcgggc cgggatttat cggccgccaa ccagccgcgc
     2401 cacctcatcc gccggcaacc catggcgcac gtgaccattg cgtcccgtca ccgtcccggc
     2461 catgagcagg ctattgtaga tggcttcctc gatgctctcg atgactgcca ccgacagcca
     2521 actcacgatc gattgctcgt cgacgaccgg tcgtggttcg ggtggcatcg gcggccgatc
     2581 ggcgatgcgg ttggtcgtgg agaaggcgat cacgaaatcg ccgctgccgc cgtggcaggt
     2641 gctgcccgtc cgcgccagac cgaacgccgc ccgccggcac aaccgcccca gccctcgcgc
     2701 gtccagcggc gcgtcggtgg ccagcaccat catgattgag ccgccgagat cgacggtctg
     2761 cccctccctt gccccctttc tccccttctc cctttctccc tttctatcgg acaccggcgg
     2821 ccgcaaccgc ctgcctaccg gcaccccgca gatcgtcagc tcttccgccc gcccgaaatt
     2881 cgtctgcacc agcgccccga cggtgtagcc acccatttcg gccggcagca cgcgcgacgc
     2941 cgtgccgata ccgcctttcc agccatagca gctcgtcccc gcccccgcgc ccaccgcgcc
     3001 ctcggcgacc tcggccgatg ccgcggccag cgccgcccgc acctcggcca ggccgatcgg
     3061 ccgcgcctgc aggtcgctca gatagccgtc gctcgtctcg cccaccaccg ggttgacgct
     3121 ggagtacccc gaccaacccg tctccgggaa gcccacgccg atatggggcg actgttcggc
     3181 catgatggtc atcagcgcgt cggccacgcg cggcacgttc atcgtgccgg tcagggcgat
     3241 gggcgtctca agattgccca gctcgcgcac ttgctcgaag cccgccacct tgccgaagcc
     3301 gttgaccgta tgcacggccg cggccacttt ttcctcatac agcttgccgt catggggcag
     3361 gatgatagtc acgccggtgc gaaaggggcc attgcccggc cgccaatccg gcgccgtgtc
     3421 gctctcgccg ctgaccagcg taaaatggcc gaccgtcacc ccggcgacgt cggtgatcgc
     3481 gttgcgcgcc cccgtgggca gtgtgctttc ataagtgatg atttctctga ttcgtggcat
     3541 gccggaagta taggcggctt gttggtggag gcaagggggg atgaggcgac actggttgca
     3601 cgaccgcagg ccgcagacca cgggctacag actacgggct acgggtaacg ggttaagctg
     3661 tggttatccc cggagaatcg gccgtgtcag gcaggtggcg ccgccctcgg ccttcagcga
     3721 catctcatct ccgcgatagg tcgctacccg gcagccggcc gcttccagcg ctgcccgcgt
     3781 ctcgtggttc tgctccagca tcacgcaatc acgcggcccc agcgccagca cgttcgtccc
     3841 catcgtggcg aactcctcat ccgagacgtc gatcagttca acgcctcgcg ctaccagcag
     3901 ctgatagaac ggcacgggca gcaacgaccg ataaatgacc gccaggtctt gatctacgag
     3961 gctgatcagc gacatcaggt gcaaacacgc ctccggcccc tcgaaatagg gcaactgcac
     4021 cggcaccagc gtgacgcccg acccggccag cgcctcgccc agttggtcga acccggcttg
     4081 attggtgcga aatcccagcc ctacacataa cgtccgctcg tcgagccaca gcaggtcgcc
     4141 gccttcggcc gtcgccgtgc cgctcaatct ataatggatg ggcacgccca gcctttggag
     4201 cgacgtcgct atcgcgtctt cctctccctc gcgcaaatac ttgcccatgc gcaggatgac
     4261 ggccccgcga tcggtcatca gcaccggatc gtggacgaag atggcgtcgg catgatccgg
     4321 cagcggcgcg tcgtggtaga ccacctcgac gccgcggccg agcagggtat tgacgaaggc
     4381 gtcatgttcc tgttgcgcca gtggcagata aggttgatcg acgtaatgcc atagcagggg
     4441 atcggccgcg ccgaaggcgg cgtcgggtcg ccgtaccaat accgtttgca ggggcgcgac
     4501 catgctttgg ccgccgtaat gttgggtcat aatggtatcc gttgaacctg cctaagggtt
     4561 ggggcgaatt accgctccag caccagtatt ccgcgctccc cggcgacgat ggcttcggtc
     4621 gccatgccca ggaacaggcc atgctccaca acgccgggct gggcgcgaat gagttgggcg
     4681 atttccgacg ggttgggcag gcccggaaaa tgacagtcga ggatgaggtt gccctcgtcg
     4741 gtgatgaagc gttctccgtc cttgcggcgc tcgacgaccc gcgcgcccag cgaagccaga
     4801 tagtcggcca ccggccgccg ggcgaactgg ataacctcca ccggcacggg cgcgcgtgtg
     4861 gccaattgtt cgactcgttt gctgtcgtcg gcgatgatga tcagccggcg cgaggccgcg
     4921 gcgacgatct tctcgcgcag gagcgccccg cccaacccct tgatgaggtt gagttgaggg
     4981 tcgatctcat ccgcgccatc gatggtgacg tcgacggagg gatagtcatc cagcg
//
LOCUS       DAOMQG010000253         4977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7105_length_4977_cov_9.672288, whole genome shotgun sequence.
ACCESSION   DAOMQG010000253 DAOMQG010000000
VERSION     DAOMQG010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7105_length_4977_cov_9.672288"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLC06_12350"
     CDS             <1..858
                     /locus_tag="PLC06_12350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91283.1"
                     /translation="GSQAVGRTDLGELLGCRLSVSFRGYDLNYVGLDNPDYYTELWRD
                     VDAVHVLGQDLWRRALKRGCPPDKPRAVISPAIDVEMFRPKEAAASRANEMAGTTERP
                     LRVLSVGRLEWKKGYEYGLAAIQHLIDDGLAVEYRIIGGGAYFEPLVFARHQMGLDGA
                     VEFLGSQPHVAVVEQMGWADLFLHPAVSEGFCNAVLEAQAMRLPVVAGDADGLPENVD
                     DGRTGFIVPRRDPAAMAEKLALLAEDGALRQAMGAAGRERVERCFTLPQQVAEFDRFF
                     REVVAGHAR"
     gene            848..>2177
                     /locus_tag="PLC06_12355"
     CDS             848..>2177
                     /locus_tag="PLC06_12355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRO91284.1"
                     /translation="MRVDLIRIGSGGAPAPAWNLGALHVAEPSPHAVAEAVARRDPAA
                     GAVLFRDAALGVPDEAAIRATLRGPGDVWHAGLSLGMGGLPRMIDFVDPVWRFNRDPG
                     LEIDATSWRLSLGACLVRVAVLDRLGGPDPHFETMAGASLELGHRWIRGGALMRHVAE
                     MIPGAASPLSLPALSLNDEIRFLRLRYGRMWTAWACWRRGRRVGGWGEAARAFRRPQA
                     PGVVAAAAYLHEPLVADKGANEVVPTVTILIPTLDRYPHLFNLLDQLRKQTVPPLEIV
                     VVDQTERERRDTRWPEEFADLPLRVLWRDVAGQCSSRNAGLVQARGDTVLFLDDDDEI
                     EPTLIDRHLTFLRRFDADASCGVAHETGAGPLPMEFSLIRDSDVFPTNNSLLRLESLR
                     RSGLFDLAYEKGERADHDLGMRLYLSGARLLLNPAAAVVHLRAPRGGLRQH"
     assembly_gap    2178..2277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2278..3672
                     /locus_tag="PLC06_12360"
     CDS             <2278..3672
                     /locus_tag="PLC06_12360"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO91285.1"
                     /translation="PARDLAGSFNIRVILFLMLHLPLAIGMEFSPWFSTAYAVLVFLF
                     GLRAALLGRVSQVIYAVAYIAAAEVLWRMTRAYVPWEYSKYAIAAIIFVALVVELSRR
                     GEARRLRSGWPLLLLVTLIPASVMAIMYYDMAEARDMISFNLSGYVALIALSLYLWNR
                     PVNRDTTVKTLLALIAPIIGVTFLAIYYTITDLDSLIFLGAANWVTSGNYGPNQVSNT
                     MGLGALAGVILFVMTPRATGARIFILILSFAMLAQGLLTFSRGGIYSFVLALAAFSFH
                     VANTPRARRRLLALFVLFGGVLIIFVYPFLDNFTGGSLTERFRDLDTTGRLGAATTDL
                     RVFSENPILGVGVGRSIEFHDTIEGISLAAHTEYTRLLAEHGLFGLLAIIIMILMLVQ
                     RYEANRPGVGRGLTAALAVWAMSIMAHSALRLATIPMAMALALVYWRLQSGTGNGAAE
                     PATDSTSADSSFTG"
     gene            3710..4879
                     /locus_tag="PLC06_12365"
     CDS             3710..4879
                     /locus_tag="PLC06_12365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91286.1"
                     /translation="MEQRPRALLFSSEFPPGPGGIGQHAYQLARHLTRLGWEIGVVSP
                     QAYVPAVERDEFNRRQPFAVTPLPDRSAGPGWFWARVRDINAAIRSRRPALLIATGQR
                     ALLMTWAFGNIHRLPWVAVGHGSEFLSRSRLNRSLTRRALENAAAVIAVSDYTAGLIR
                     AAAAPRRLLVIPNAADGERFRPGLSTAALRQRWDIGEGPVVLTVGQVSERKAQDVIIR
                     ALPRVLARHPELTYVMAGLPTLKEPFAALAAELGVTDHVRFVGIVADEELPATYNLAD
                     VFALVSRKTAAGDVEGFGIVVQEAALCGVAAVVSRDCGLTEAIREGETGLSVPPDDPE
                     STAAAILTLLGDDSRRREMGHRARKLAQAATWAERINEYDRALRAVLPLRPKLSH"
BASE COUNT          757 a         1464 c         1611 g         1045 t
ORIGIN      
        1 ggctcgcagg ctgtcggccg cactgacctg ggcgaattgc tcggttgccg cctgagcgtc
       61 agtttccgtg gttacgacct gaattacgtc ggcctcgata accctgatta ttacaccgaa
      121 ttatggcgcg atgtggacgc cgtccatgtt ctggggcagg acttgtggcg gcgagcctta
      181 aagcgcggtt gcccgcccga caagccgcgc gccgtcatct cgccggccat cgatgtagag
      241 atgtttcgcc cgaaggaggc cgctgcttcg cgggccaatg agatggcagg aacgacagaa
      301 cggccgttac gtgtgcttag cgttggtcgt cttgagtgga aaaaagggta cgaatatggc
      361 ttggccgcga ttcaacatct gatcgatgat ggcctcgcgg ttgaatatcg catcatcggc
      421 ggcggcgcat attttgaacc gttggtattt gcccgacatc agatgggcct cgacggtgca
      481 gtggaatttc ttggctccca gccgcatgtt gccgtcgtcg agcagatggg ctgggcggat
      541 ctgttcctgc atccggccgt gtccgagggc ttttgtaacg ccgttctcga ggcccaggcc
      601 atgcgtctgc ccgtggtggc cggcgatgcc gacggcctgc cggagaatgt ggatgacggc
      661 cgcaccggtt tcattgtgcc ccggcgcgat ccggcggcga tggccgagaa gctggcgtta
      721 ctggccgaag acggcgcgtt gcgccaggca atgggcgcgg ccgggcggga gcgggtggag
      781 cgttgcttca ccctgccgca gcaggtcgcc gaatttgacc ggttcttccg ggaggtggtg
      841 gccggccatg cgcgttgatc tgatccggat tgggtccggc ggcgctccgg ctcctgcctg
      901 gaacctgggc gcgctccacg tcgctgaacc atcgccgcac gccgtggccg aggctgttgc
      961 tcgccgcgat ccggccgccg gcgccgtcct gttccgggac gcggctctgg gtgtgccgga
     1021 tgaggcggcg atccgggcaa ccctgcgtgg gccgggcgat gtgtggcacg ccggactgtc
     1081 gttgggcatg ggtggattac cgcggatgat cgatttcgtc gatccggtgt ggcgtttcaa
     1141 tcgcgatcca gggctggaaa tcgatgccac ttcctggcgg ctctcgctgg gcgcgtgtct
     1201 ggtgcgggtg gccgtgctcg atcggttggg tgggccggat ccgcacttcg agacaatggc
     1261 cggcgcgtcg ctggagctgg gccatcgctg gattcggggc ggcgcgctga tgcgccacgt
     1321 cgccgaaatg attcccggag cagcttcccc gttgtccctt cctgccttgt cgttgaatga
     1381 tgagatccgc ttcctgcgcc tgcgctatgg ccgaatgtgg acggcgtggg cctgctggcg
     1441 gcgcgggcga cgcgtcggcg ggtggggtga ggcggcgcga gcctttcgtc ggccgcaagc
     1501 gcccggtgtt gtggccgcgg cggcttatct ccacgaaccg cttgttgccg ataagggtgc
     1561 gaatgaggtt gtcccgaccg ttacaatcct gatcccgacg ctcgaccgct atccgcacct
     1621 gttcaacctg ttggaccaat tacggaagca gaccgtcccg ccacttgaaa ttgttgttgt
     1681 ggaccagacg gagcgcgagc gccgcgacac gcgctggccg gaagagtttg ccgatctgcc
     1741 gctgcgcgtt ttatggcgcg atgtcgccgg gcagtgttcc tcgcgcaacg cggggttggt
     1801 gcaggcacga ggcgacacag tgctgttcct ggacgacgac gacgagatcg agccaaccct
     1861 gattgaccgc catctgacct tcctgcgacg tttcgatgcc gatgcatcat gcggcgtcgc
     1921 ccatgagact ggagctggtc ccctgcccat ggagttcagc ctcatccgtg acagcgacgt
     1981 gtttccgacc aataattcgc tgctaagatt ggagtcactg cgccgttcgg gcctgttcga
     2041 tctggcctat gagaaagggg agcgggcgga tcacgatctg gggatgcgcc tctacctgtc
     2101 cggcgccagg ttgctgctca atccggcggc ggcagtcgtc catttgcggg cgccccgcgg
     2161 cgggctgcga cagcacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg
     2281 gcgcgcgatc tggccggatc attcaacatc cgggtcatcc tgtttctgat gctccatctg
     2341 ccgctggcca ttggaatgga gttctctcca tggttcagca cggcctatgc ggtgctggtg
     2401 tttctcttcg gcctgcgagc cgcgcttctg gggcgcgtgt cgcaggtgat ctacgccgta
     2461 gcctacatcg ctgccgctga agttctgtgg cgcatgacgc gggcctatgt gccatgggag
     2521 tattcgaaat acgctatcgc ggccattatc ttcgtggccc ttgtcgtcga attgagccgg
     2581 cgcggcgagg cacgccgatt gcgcagcggt tggccgctgc tgctgctggt gacgctgatc
     2641 cccgcgtcgg ttatggccat catgtattat gacatggccg aggcccgtga catgatcagc
     2701 ttcaatctgt ccggctatgt cgctctaatc gccctgagcc tttatctgtg gaaccggccg
     2761 gtcaatcgtg atacgacggt taaaaccctt ctggcgctca tcgctcctat catcggcgtt
     2821 acctttctgg ctatttatta cacgatcact gacctggaca gcctcatatt cctgggcgca
     2881 gcaaactggg tgaccagcgg aaattatggc ccgaaccagg tctccaatac aatggggctg
     2941 ggcgcgctgg cgggggtcat tctgtttgtc atgacgccac gcgcgacggg ggcacggatc
     3001 tttatcctga tcctttcttt tgccatgctg gcccaaggcc tgctcacctt ttcacgcggg
     3061 ggcatctata gctttgtgtt ggcgctggcc gcgttctcgt tccatgtggc caatactccg
     3121 cgggcgcgac gacgattgtt ggcgctgttc gtgctcttcg gcggtgttct aatcatcttc
     3181 gtctatccgt tcctcgataa tttcactggc ggctccctga ccgagcggtt ccgcgatctg
     3241 gacacgactg gtcgcctggg ggcggcgacg accgacctgc gggtcttcag cgagaatccg
     3301 attctgggcg taggcgtggg tcgatcgatt gagtttcacg acacgatcga aggcatctcg
     3361 ctggctgccc acacggagta cacccgacta ttggccgaac atggcctttt tggcctgctt
     3421 gcaattatca ttatgatctt gatgctggtt cagcgttatg aggccaaccg accaggggtg
     3481 ggtcgggggc tgacggccgc gcttgcagtc tgggcgatgt cgatcatggc tcatagcgcc
     3541 ttgcgtctgg ccacgattcc gatggccatg gcgctggcgc tggtctattg gcgattgcaa
     3601 tcgggcaccg gcaacggcgc ggccgagccg gcgaccgatt ccacgagcgc ggactcatca
     3661 ttcaccggct aacgggcaaa ccgatatggt atttgacgcc acagacccga tggaacaacg
     3721 accgcgcgcg ctgttgttca gcagcgagtt cccacccggc ccggggggaa ttggccaaca
     3781 cgcctatcag ttggctcgcc atctgacgcg cctggggtgg gagatcggtg tggtgtcgcc
     3841 ccaggcctat gtcccggccg tcgaacggga cgaatttaat cggcgacaac cgttcgccgt
     3901 cacaccgctg ccggatcgct ccgcggggcc tggctggttt tgggccaggg tacgagacat
     3961 caacgccgcc attcgttccc gccggcccgc gctgctcatc gccaccggtc aacgagcgct
     4021 gctgatgacc tgggcgtttg gcaatatcca tcgattaccc tgggtggctg tggggcatgg
     4081 cagcgaattc ctcagcaggt cgcgcctgaa ccggagcctg acccgtcgcg ctctggagaa
     4141 tgcggcggcc gtcattgccg tcagcgatta caccgccggg ctgattcgtg ccgcggccgc
     4201 cccgcgacga ctgctggtta tccccaatgc cgccgacggc gaacgctttc ggcccggact
     4261 gtcgacggcc gcactgcgac aacggtggga tatcggcgaa gggccggtgg tgttgacggt
     4321 ggggcaggtg agcgagcgca aggcgcaaga tgtcatcatt cgtgctctgc cgcgcgtcct
     4381 cgccaggcat cccgaactca cctatgtgat ggccgggctg ccgacattga aagagccgtt
     4441 cgccgcgttg gccgccgaac tgggcgtaac cgaccacgtg cgctttgttg ggattgtcgc
     4501 cgatgaggag ttgcccgcga cttataacct ggccgatgtg tttgcgttgg tcagccgcaa
     4561 gacggccgcc ggcgatgtgg agggattcgg catcgtcgtc caggaggcag cgttgtgtgg
     4621 tgtggccgcc gtcgtcagtc gcgattgcgg actgacggag gcgatacgcg agggagagac
     4681 cggtctcagc gtgccgccgg acgatcctga gtcgacggcc gcagctattc tgaccttgct
     4741 tggcgatgat tctcgccgcc gggaaatggg tcaccgcgcc cgcaaactgg cgcaggccgc
     4801 cacctgggcg gagcgaatca atgaatatga tcgggccttg cgcgccgtat tgccactgcg
     4861 cccgaaactg tcccattgag ggatcggagt tttgaccatg cgcttgctca tcgtctccca
     4921 caccccccat tatcatgccg gtgaccaaat tgttggttgg ggtccgacgg tgcgcga
//
LOCUS       DAOMQG010000254         4969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7121_length_4969_cov_10.746846, whole genome shotgun sequence.
ACCESSION   DAOMQG010000254 DAOMQG010000000
VERSION     DAOMQG010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7121_length_4969_cov_10.746846"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLC06_12370"
     CDS             <1..443
                     /locus_tag="PLC06_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRO91287.1"
                     /translation="TITLATAALFAPTLRQKAAEFAQLTGCNVSVVEIRNERLGETIT
                     VAGLLNGVDVLAQLREVNPGELLVLPRVMFDHPDRISLDNISPQDIANQLDRPVALAD
                     SLGDVWDALLGQSKLMYRPGVPPAGTINLKVLGNGANGSAAHLS"
     gene            complement(471..3668)
                     /locus_tag="PLC06_12375"
     CDS             complement(471..3668)
                     /locus_tag="PLC06_12375"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRO91288.1"
                     /translation="MNIIKFFRFGLPTTNAEFNHSNMRPRHRRMGIILPLVLVLAFLT
                     TGLAHGDYAQREFTAESVINLSSLVGPSGFRIDGHNDGDQAGRSLGFAGDVNGDGLND
                     LIIGAPSANPENTQNAGEAYVIFGRTTNPASLPLGDLNGTNGFRIVGVTFNDEAGSAV
                     SGGGDVNNDGYDDLIIGAPGADLDELTPEAGAVFVVFGQASFPAKVDLNSLNGASGFR
                     MNGIVMDDRAGAGVNLAKDVNGDGFDDILIGAPDASPGGKQGAGQIYVVWGKQSFPPV
                     FNLSTLNGTNGFIVDGVASETSAGKILRMAGDLNKDGYGDFLLSAGSHLSGRSLDTGT
                     VFVVFGQQTFPAKFNLAGINGTNGFRIEGIADGDNAGRAVAAAGDVNGDGRDDLVIGA
                     PYASDNKGEAYIVFGRTTYSAAINLKDLNGLNGVRLIGDQANGEAGTAVSAADINGDG
                     LQDVVIGASAAGSGSSRLAGMTYVVFGRANFGAPSLSLGALGAGGLRFDGAASSDQSG
                     QTLGHAGDFDGDGYDEILIGAPNAGPGVKSKAGFIYLVQGGPTLGVSMPATHSGTPGN
                     NNLSGTSGNDVMLAHRGDDQINAVAGNDVLKGGAGNDIHNGGSGADRIIGGTGKDTAS
                     YADSAAGVNINLFTGIIGGGDATGDSLVSIEHLIGSNAADTLIGDTRDNMLTGAGGSD
                     SLTGGLGNDTFMYSAGSGADTITDFMPGSQTPDLLDFTGYPAITGVGSLLINPQGADT
                     LITLPGSETILLKGVTPGQLHADDYRFTGMPVAKPDHFTTSANTELQVNAPGVLANDV
                     NPGAGQLTAVIVNNPTHGTLTLQANGGFTYTPNKDFIGGDEFAYQANNGQLSNITQVT
                     IDVTPIPPTALDDSFSVDLGDTLSVPAPGVLANDLKGGNLPMQAILVEEPVGGSLTLN
                     SDGSFTYTPDADYDTQDSFTYQASNGLLSNIATVTIAIIIPDGPPVAFDDAYELSAGD
                     TLVIPASGVLENDINPLAGVMTAKLVDKPEHGSLALNANGGFSYQPNTGFVGQDHFTY
                     RADNGQLSNIATVSLEIISTPNQMQRVMLPVVLYH"
     gene            4125..>4969
                     /locus_tag="PLC06_12380"
     CDS             4125..>4969
                     /locus_tag="PLC06_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91289.1"
                     /translation="MPILIGIAFDAALAHPPDMGIIGWAALGIFVSQVVRAVLQLGRN
                     FASSVIGERLERDTRQELYISLLGKSMTFHDLQPVGDTMARATNDVRELNLMMYPGVN
                     LVVGSANFLIMPLLLAPRYHWQLIFAPLFFIVSYVIALAQYMNVLSRIADRVRTAFGV
                     LNARLAEAIDGIEMVKSTAQETAEVERFETNARAFRDATVAQGREEAKFIPLLLLGIT
                     EAIGFAHALILLHAGQIQVGDVVAYLGLLSLFGFPTNISLMAYSRVSLGMAGARRILE
                     LINTET"
BASE COUNT         1018 a         1393 c         1419 g         1139 t
ORIGIN      
        1 gaacaattac gctggccact gcggcacttt tcgcgcccac gctcaggcag aaggctgcgg
       61 agttcgccca attgaccggt tgtaatgtca gcgttgtgga gattcgcaac gagcgattgg
      121 gggaaaccat cactgtcgcc ggcttgctta acggggtcga cgtgctggcg cagttgcgcg
      181 aggtcaatcc gggcgaattg ctcgttctcc cacgggtgat gttcgatcat cccgaccgga
      241 tctcgctgga taatatatct cctcaggata ttgctaacca gctcgatcgc cccgtagcgt
      301 tggccgactc gctgggtgat gtgtgggacg cgctgctcgg ccaatcgaaa ttaatgtacc
      361 ggccaggtgt gcccccggcc ggtacgatta acctgaaggt attgggcaac ggcgcgaacg
      421 gatcggccgc ccatttatcc taaaccgccc cagcctctgc tgcttacaac ttaatgatat
      481 aaaaccacag gaagcatgac ccgctgcatc tgatttggcg tgctaatgat ttccaggctg
      541 acagtggcga tgttggatag ctgcccatta tccgcccggt acgtaaaatg gtcctggccc
      601 acaaatcctg tgttcggttg ataagagaac cccccattgg cgttcagcgc cagtgacccg
      661 tgctcgggtt tgtccacaag cttggctgtc atcactccgg cgagtgggtt gatatcgttt
      721 tccagcactc ctgatgccgg tatgacgagc gtatcgccgg cgctgagttc atatgcatca
      781 tcgaacgcaa caggcggacc atcaggaatg atgatcgcta tcgtgacggt agctatgttg
      841 gagagcagac cattgctcgc ctgataggtg aaactgtcct gggtgtcgta gtcggcatcg
      901 ggcgtatagg tgaacgagcc gtcactattc agggtcaggc taccgccaac aggctcctca
      961 acgaggattg cttgcatggg gagattgccc cctttcagat cgtttgccag cacgccgggc
     1021 gcgggaaccg acagggtgtc gcctagatcc acagaaaacg aatcatccaa cgccgttggc
     1081 ggtataggcg tgacgtcgat cgtcacctgg gtgatattgg agagttgccc attgttggct
     1141 tgataagcga attcatcgcc gccgatgaag tccttgtttg gggtataggt gaacccgccg
     1201 tttgcctgaa gagtcaatgt gccgtgggtg gggttattaa cgatgacggc cgtcagttga
     1261 ccggcgcccg gattgacgtc gttggccagg actcccggcg cgttgacttg tagctcagta
     1321 ttagccgacg tagtgaaatg atccggcttg gccacaggca tgccggtaaa tcgatagtcg
     1381 tcggcatgga gttggccggg ggtaacaccc ttaaggagaa ttgtctcgct acccggcaac
     1441 gtaatcaacg tatcggcccc ctgcgggttt atcaggaggc tgcccacacc agtgatggct
     1501 ggatagccgg tgaaatcgag aagatcaggc gtttgggaac ccggcatgaa atccgtgata
     1561 gtgtctgcgc cgctgccggc gctatacatg aaagtgtcat tgcctaatcc gccggtcagg
     1621 gaatcgctgc ctcctgctcc ggtcaacata ttgtctctcg tgtcaccaat cagggtgtcg
     1681 gcagcgtttg aaccgatcaa atgctcgatg gaaaccagcg aatcgcccgt cgcatctccg
     1741 ccgccgatta tcccggtgaa caggttgatg ttgactcccg cggccgaatc ggcatatgat
     1801 gctgtatcct tccccgtgcc gccaataatc cgatcggccc cggaaccgcc attgtggatg
     1861 tcattccccg cgccgccttt gagaacgtcg ttccccgcaa cggcattgat ctggtcgtcg
     1921 ccccgatggg caagcatgac gtcgttcccg ctcgtgccgg agagattgtt gtttcccggc
     1981 gtgccggagt gggtcgcggg catgctgacc cctaatgtcg ggccgccttg tacaagatag
     2041 atgaatccgg ccttggactt aacccctggc ccggcatttg gcgcgccgat gagaatttca
     2101 tcgtacccgt cgccatcgaa gtcacccgcg tgacccagcg tctggccgga ttgatcgctt
     2161 gaagcggcgc catcgaaacg cagcccaccg gctcccagcg cgccaaggga aagagaagga
     2221 gcgccgaagt tggcgcgacc aaagacgacg taagtcatgc ccgcaagtcg tgagcttccg
     2281 ctcccggcgg ccgatgcgcc gatgaccaca tcttgcaggc cgtcgccgtt gatgtcggcc
     2341 gcgctgaccg ctgttccggc ctcgccattg gcctggtcgc cgatcaggcg aactccattc
     2401 aggccgttca gatctttcag attgatagcc gccgaatagg tagtgcggcc gaagacgatg
     2461 taggcctctc ccttgttgtc actcgcatac ggcgcgccga tgaccagatc gtctcggccg
     2521 tcgccattca catcgccggc ggcggcgacg gcgcgacccg cattgtcgcc atcggcgatc
     2581 ccttcgatgc gaaagccgtt ggttccattg atgccggcga ggttgaactt ggccggaaaa
     2641 gtttgttgac cgaaaaccac gaaaaccgtc ccggtatcca gcgatctgcc tgaaagatgg
     2701 ctcccggccg ataacagaaa atcgccatag ccgtccttgt tcaggtcgcc ggccattcgc
     2761 aatattttac cggccgaggt ctcggaagct acgccatcca caatgaaccc atttgtgccg
     2821 ttcaatgtgg ataagttgaa cacgggcggg aaggattgct tgccccagac tacatagatc
     2881 tgcccggcac cctgctttcc tcccggtgaa gcgtccggag cgccgatgag gatatcgtcg
     2941 aatccgtccc cattgacgtc tttcgccagg ttgacgccgg caccggctcg atcgtccatg
     3001 acaattccat tcatgcggaa acccgatgca ccattgagcg agttaaggtc gacttttgcc
     3061 gggaaggaag cctggccgaa aaccacgaac acagctcccg cttcgggagt cagttcatcc
     3121 agatcggctc ccggcgcgcc gatgatcaaa tcgtcatacc cgtcgttgtt gacgtctccc
     3181 ccgccactga cggcgctgcc tgcctcatca ttaaaggtga cgccgacaat cctgaacccg
     3241 ttcgtcccat tcagatcgcc gagaggcaag gacgcaggat tggtggttcg accaaagatg
     3301 acataggctt ccccggcatt ttgtgtgttt tccgggttgg cagaaggtgc cccgataatg
     3361 aggtcgttga ggccatctcc gttgacgtct cccgcaaagc ccagagaacg tccggcttgg
     3421 tcgccatcat tatgaccatc aatccgaaag cccgacgggc cgaccaggga agagagatta
     3481 attaccgatt ccgccgtgaa ctctcgttga gcatagtcgc catgagcaag gcccgtcgtc
     3541 aaaaaggcta acacgagaac caatggcagg atgatgccca tccgtctatg tctggggcgc
     3601 atgttggagt gattgaattc agcgtttgtt gtgggcagtc caaaacggaa gaatttgatg
     3661 atattcattc aacatttcct tgttatcggt tgatagtttg ttcatttcag aaaaggtcga
     3721 gttccgatag ggatgtattg agttgcgcag ttaattcgga aactccccaa gtttgagacg
     3781 agccgagagg ctattatcgg gcaaaagggg gcgcataagg ataccgagtt atagccaagc
     3841 ggcttggata ttagccaaaa aacgccgaag ggcaacttga tttcgggtgg cccacaacgt
     3901 gaccgaatcg gaagttatgg gataattggc cgtctttacg gctattgtcg acggtatttt
     3961 ggaacggaac agtttgtttt atgggtgaat ttgcgattca agggcatgcg atcgtccggc
     4021 accgctctgc gggacgatgg atattgtcgc acctgaccaa acaatggcaa ttattccttg
     4081 tcatgttcat cggcgcgttc gggaatgcgg ccctggcggc cgttatgcct attttgatag
     4141 gcatcgcgtt cgacgccgcg cttgcccatc cgcctgatat gggcatcatt ggctgggccg
     4201 cgcttggcat ttttgttagc caggtcgtcc gggccgtttt gcaacttggc cgaaatttcg
     4261 ccagttcggt tatcggggag cggctggagc gcgacactcg tcaggaactc tacatcagct
     4321 tgctgggcaa gagcatgacc ttccatgatc tacagcctgt gggtgatacg atggctcggg
     4381 caaccaatga cgtgcgcgag ctgaatctca tgatgtatcc cggcgtcaat ctggttgtcg
     4441 gctcggccaa ttttctaatc atgccgctct tgcttgcgcc acgctatcac tggcagctca
     4501 tctttgcccc gcttttcttc atcgtctcct atgtcatcgc attggcccag tacatgaatg
     4561 tcctgtcgcg gattgcagat cgcgtgcgga cggcctttgg cgtgcttaac gcgcggttgg
     4621 cggaggcaat cgacgggatt gagatggtca agtccacggc acaggaaacg gccgaggtcg
     4681 agcgcttcga aaccaacgcc cgcgcctttc gtgatgcgac tgttgcccag ggtcgcgaag
     4741 aagccaaatt catccccctt ctgctgctgg gaattaccga agctatcgga ttcgcccatg
     4801 cgctcattct gctgcacgcc ggccaaatac aggtcggtga tgtcgtagcc tatttgggtt
     4861 tattgtcact ttttggcttt ccaaccaata tctcgctgat ggcctattcg cgggtatctc
     4921 tgggcatggc cggcgcccgt cgtatcctgg agctcattaa caccgagac
//
LOCUS       DAOMQG010000255         4945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7158_length_4945_cov_7.535174, whole genome shotgun sequence.
ACCESSION   DAOMQG010000255 DAOMQG010000000
VERSION     DAOMQG010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7158_length_4945_cov_7.535174"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLC06_12385"
     CDS             <1..546
                     /locus_tag="PLC06_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRO91290.1"
                     /translation="GYETVADNPYLADFYDDMRQWSFHLQVFFLGHRAQQHLALATQP
                     NSAIADRSIYEDAHIFARVLRHMGNLSERDYESYRHVYELVVANLPRPDLLLYLTAPV
                     PVLMDRIRQRARAIETGITPDYLTLLDTFYDDWLAGFDLCPVLAIRTQQLDYVHDARH
                     LDIVIERIEDKLAGREELVFD"
     gene            619..1266
                     /locus_tag="PLC06_12390"
     CDS             619..1266
                     /locus_tag="PLC06_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91291.1"
                     /translation="MNEETGGERPKLPRTTHLLIFLLIVGLALAGWIGYSTCALGSVA
                     PAATQTAPTQVGPTQVATAVVDAPGPADAPGDGFGIVAGQATPWAVPGGPGELPTPEA
                     TPAPPVAVPITLIGPPPDSFFRMGDSVTFYWSSPEPLAPGQQFTVYALADGAQIALGS
                     IAEPNLGLGYQLHVVPGPVTGRPGPFNWSIALEDQASGAIIGQSAVRGITLIANN"
     gene            1414..3264
                     /locus_tag="PLC06_12395"
     CDS             1414..3264
                     /locus_tag="PLC06_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRO91292.1"
                     /translation="MVARDKEDLSGQQIGNYEIESHIAARKASDLFLARDVKLERLVF
                     LEVLRVTDDEDEDLAERFRGRMETVSQLKDPHIAVVSDFDITDDGFPYAVIDYFPGET
                     LADRLADLRRDKQTAPVIESLELIKQIAQGLGVAHAAGLIHHDLRPQNIMLREDGTPI
                     LIDLGLPIVAGPSIRGLNSADKEHLDYASPEELEGKPLTRRSNIYSLGVILYELLAGH
                     QPNLPTLPFDIFPQANMPKEEPLEDARPGLAGETYRLVRNCLWRQEWSRFETTDEMIT
                     AIETAIFAEQELPKAAAWAPKRGNSTRIIIPIAVILAGVLLFFLLRGILGNGKAQANE
                     PTPVDTLAGAAETTGTSTATSTPTTNPSGNLSISVLPHGPNRVFTAQDTINFDWFWPT
                     LPEVGQQFAVYLLVGDERHLLGTTTEPYSGAAYRLSVPAARLPASGDLNWQVRLETIV
                     DGEPVVESDPTLIVVRLETPTPTSSATVKATGTAATTGTPTCVVSPPPGWVQYTIRQG
                     DALSDLAERADIPVESLLEVNCLPDVSLSVGQQVWVPQASVPRTPTPQPAPATATQQP
                     RPTATTGGNTPDPRPPTDTPSQPTPTEPPAATEPPPTFTPPPDPTEPPSG"
     gene            3660..>4945
                     /locus_tag="PLC06_12400"
     CDS             3660..>4945
                     /locus_tag="PLC06_12400"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HRO91293.1"
                     /translation="MKNRAIVLNLGSLFVALFLIVILTVPVFAAKPIILPPYTLSIAE
                     NAVPGNTTTPNKINAFDAEPGLLEFQKLGGDGMTMFDIGFNTGIVTLAAGQSLDYETK
                     KSYTLDVQVQDAELLTDTRTITINVTDVPDETPVLADGQSFNVAENSPNNTLVGKLLF
                     SDADTVDSFTFIIESGNGSGGGAFLIEPDAEIRVKDSAQLDHETTPQFLLEVKVTDSG
                     GNFDTTTVIVNVTNINEKPIISNQSFSGVSEAAPNGTSVGFIVATDPDGDTLGFMRTS
                     GSAAFAVNGTTGEVTVADNTLLDFETQPAPTFVVTVNDGKGQSSSATITVNLVDVNDT
                     PRVIGGGIPDVIINQGTLVATRNLWNYFADDEDANSEMSYTIKDNTNPGLFSAAPTID
                     NGTGVLTMNFAANAAGEAALTIRATDMDGAFVDDTF"
BASE COUNT          990 a         1837 c         1307 g          811 t
ORIGIN      
        1 ggctacgaaa ccgtcgccga caacccctac ctggccgact tctacgacga catgcgccaa
       61 tggtccttcc acctgcaagt cttcttcctc ggccaccgcg cccaacaaca cctggccctg
      121 gccacccagc ccaactccgc catcgccgac cgcagcatct atgaagacgc ccacatcttc
      181 gcccgcgtcc tgcgccacat gggcaacctc agcgagcgcg actacgaatc ctatcgccac
      241 gtctacgagc tggtcgtggc caacctgccc cgccccgacc tgctcctcta cctgaccgcc
      301 cccgtccccg tcctcatgga ccgaatcaga cagcgcgccc gcgccatcga gaccggtatc
      361 acccccgact acctcaccct gctcgacacc ttctacgacg actggctggc cggcttcgac
      421 ctctgtcccg tcctcgccat ccgcacccaa caactcgatt acgtccacga cgcccgccac
      481 ctcgacatcg tcatcgaacg catcgaagac aagctcgccg gccgcgagga actcgtcttc
      541 gattgagcat gacccactcg ccatgctcgc ctgcttgtgt taaacttcac aaattaacaa
      601 accggaaccc agtgccctgt gaatgaagag accggcgggg agcggccaaa acttcccagg
      661 acaacccatc tgctcatctt cctgctgatc gttgggctgg ccctggccgg gtggatcggc
      721 tacagcacct gcgcgctcgg ctcggtcgcg ccggcggcaa cgcagaccgc gccaacgcaa
      781 gttgggccaa cgcaggttgc aacggccgtg gtcgacgcgc ccggcccggc cgacgcgccc
      841 ggcgacggct tcgggatcgt cgccggacag gccacgccgt gggccgtccc cggcgggccg
      901 ggcgaactgc ccacccctga ggccacgccc gcgccgcccg tcgccgtccc aatcacgctc
      961 atcggcccgc cccccgacag cttcttccgc atgggcgaca gcgtcacctt ctactggtca
     1021 tcgcccgaac cgctcgcccc cggccaacag ttcaccgtct acgcgctggc cgacggagcg
     1081 cagatcgccc tgggatccat cgccgaacca aacctgggcc tgggctacca actccacgtg
     1141 gttcccggcc ccgtcacagg gcggccgggg ccattcaatt ggtcaatcgc cctcgaagat
     1201 caggcgtccg gcgccatcat tgggcaaagc gcggtcaggg gaatcaccct gatcgccaat
     1261 aattaacggc agaatcacgc caaaagcacg aagatcgaca cgtattgcat tttgtcgtgt
     1321 ataatagtac gctattaatg ggccgcgttg ctgggtaagg agcgctcacc gactggcggc
     1381 aacttccggg tgcggcgagt acgtacactg agtatggtgg caagggataa agaagacctg
     1441 tccggtcagc agatcggaaa ctatgagatt gaatctcaca ttgcggcgcg caaggccagc
     1501 gatctgttcc tcgcgcgtga cgtcaagctt gagcgtctcg tgtttctcga agtcctgcgc
     1561 gtcacggacg atgaagacga agacctcgcc gagcgcttcc gcggccggat ggagaccgtt
     1621 tcccagctga aagatcccca catcgccgta gtcagcgact tcgacataac cgacgacggc
     1681 ttcccctatg ccgtcatcga ctacttcccc ggcgaaacgc tggccgacag actggccgac
     1741 ctgcgccgcg acaagcagac cgccccggtc atcgaatcgc tcgagctgat caaacagatc
     1801 gcccagggcc tcggtgtcgc ccatgccgcc gggctgatcc accacgatct ccggccacag
     1861 aacatcatgc tccgcgagga cggcacgccc atcctcatcg acctcggcct gcccatcgtc
     1921 gccggcccat cgatccgcgg cctcaatagc gccgacaagg agcatctcga ctacgcctcg
     1981 cccgaagagc tggaaggcaa gccgctgact cgccgcagca acatctactc gctgggcgtc
     2041 atcctctacg aactcctggc cggccaccag cccaacctgc ccacgctgcc cttcgacatc
     2101 ttcccccagg ccaacatgcc caaggaagag ccactcgagg acgcccgtcc cgggctggcc
     2161 ggcgaaacct atcgcctggt gcgcaactgc ctctggcggc aggaatggag ccgattcgag
     2221 accaccgacg agatgatcac cgccatcgag acggccatct tcgccgaaca ggagctgccc
     2281 aaggccgccg cctgggcgcc gaagcgtggc aactcgacga gaatcatcat ccccatcgcc
     2341 gtcatcctgg ccggggtgct cctgttcttc ctgttgcgcg gcattttggg caatggcaag
     2401 gcccaggcca acgagccgac gccggtcgac acgctggccg gcgccgccga aacaaccggc
     2461 acatcgaccg ccacctccac gccaaccacg aacccgtccg gcaacctctc gatctccgtg
     2521 ctgccccatg gccccaaccg ggtgttcacc gcccaggaca ccatcaactt cgactggttc
     2581 tggcccacgc tgcccgaagt gggccaacaa ttcgccgtct atcttctggt gggcgacgag
     2641 cgccacctgt tgggcacgac gaccgaaccc tatagcggcg ccgcctatcg cctgtccgtg
     2701 cccgccgccc ggctgcccgc gtccggcgac ctgaactggc aagtgcgact ggaaaccatt
     2761 gtcgatgggg aacccgtcgt cgagagcgac ccgaccctca tcgttgtgcg gctggagacg
     2821 ccgacgccga cctcatccgc caccgtcaag gccaccggaa cggccgccac caccggcacc
     2881 ccaacctgcg tcgtctcccc gccccccggc tgggtgcaat acacgatccg tcagggcgac
     2941 gctctctccg acctggccga acgagccgac atcccggtcg aatccttgct cgaagtcaac
     3001 tgcctgcccg acgtctccct gagcgtcggt cagcaggtgt gggtgccaca ggcctccgtc
     3061 ccgcgcaccc ccacgccgca gcccgctccc gccaccgcca cccaacaacc gcggccgacg
     3121 gccaccaccg gcggcaacac ccccgacccg cgcccgccca cggacacacc ctcgcagccc
     3181 acacccaccg aaccgccggc ggccaccgaa ccgcccccga cgttcacccc gccccccgat
     3241 cccaccgagc cgccttccgg ctgatgccct tcaacaaccc gggccggcgc gccaacctgc
     3301 ccccacattt ccatgatcga cctgtcggat cgtccgactt ttcgcgctcc ccaattcgcc
     3361 attgacaaaa ttgtgtgaaa agcgttagta ttcccgcatg gaaggtagta ccgaagtact
     3421 attacgtggt cgatacggac atctgtccta tcgttcatcc ggaaccgggc gggagtcgtt
     3481 ggctcggatt ctttcacact attttaaccg ccggtgcgcg gcataatcgg taacattgag
     3541 agcggcgtct cgcatcgtcg catctcgttt gggcaccgat tggggccgaa tggagcgatc
     3601 gggatgaaac tcggttccaa aaacgcgcct tcccggcgtc acaggagtgc aggagcatca
     3661 tgaaaaatag agccatcgtt ctgaacctgg ggtcgttgtt tgttgccctc tttctgattg
     3721 tgatactaac cgtgccggta ttcgcggcca aacccataat ccttcctccc tatacactca
     3781 gcattgccga gaacgcggtt cccggcaaca ccaccacccc caacaagatt aatgccttcg
     3841 atgccgaacc tgggcttttg gagttccaga agctgggcgg cgacggcatg accatgttcg
     3901 acataggttt caacacgggc atcgtgacgc ttgccgccgg ccaatcgctg gattacgaaa
     3961 ccaagaaaag ctacaccctc gatgttcagg ttcaggatgc cgagttgctg accgataccc
     4021 gaaccatcac catcaacgtg accgacgtcc ccgacgaaac cccggtgctg gcggacggcc
     4081 aatcgttcaa cgtggctgag aacagcccca acaacacgct ggtgggcaag ctcctcttct
     4141 ccgacgccga taccgttgat tccttcacct tcatcatcga gagcggcaac ggcagcggcg
     4201 gcggcgcgtt cctcatcgag cctgatgccg aaatccgggt caaggacagc gcccagcttg
     4261 accacgaaac gacgccccag ttccttttgg aagtcaaggt gaccgactcc ggcggcaatt
     4321 tcgacacgac caccgtcatc gtcaacgtca ccaacatcaa cgaaaagccg atcatcagca
     4381 accagagctt cagcggcgtc agcgaggccg ccccgaacgg gacttcggtc ggcttcatcg
     4441 tggccaccga ccccgacggc gacaccctgg gattcatgcg cacttccggc tcggccgcct
     4501 ttgcggtcaa cggcaccacc ggcgaggtga ctgtcgccga taacaccctg ctcgatttcg
     4561 agacccagcc cgcgcccacg ttcgtggtca ccgtcaacga cggcaaaggc caaagcagct
     4621 ccgccaccat caccgtgaac ctcgtcgacg tgaacgacac gccgcgagtg atcggcggcg
     4681 gcatccccga tgtcatcatc aaccagggca cgctcgtcgc cacccgcaac ctgtggaact
     4741 acttcgccga cgacgaagac gccaacagtg agatgtccta cacgattaag gacaatacta
     4801 acccgggcct tttctcggcc gcgccgacga tcgataatgg caccggcgta ctgacgatga
     4861 actttgccgc caacgcggcc ggcgaggccg ccctcaccat ccgcgccacc gacatggacg
     4921 gcgcgttcgt cgacgacacc ttcaa
//
LOCUS       DAOMQG010000256         4918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7200_length_4918_cov_10.024265, whole genome shotgun sequence.
ACCESSION   DAOMQG010000256 DAOMQG010000000
VERSION     DAOMQG010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7200_length_4918_cov_10.024265"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1358
                     /locus_tag="PLC06_12405"
     CDS             372..1358
                     /locus_tag="PLC06_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRO91294.1"
                     /translation="MKSLQNTRIILGALLGALMLILAFAAIPTASAAPLRQAPTPEPN
                     AQECVGCHEGLRGYWDQSAHGYALTDANFQKAWTEQGSPNECLACHTTGYDPETGSYT
                     GGGVGCLSCHSPVPSNHPEAYMPTDVSSRLCGNCHIDTFAEWEISEHGEQGMTCNQCH
                     NPHTAKLRVGTSQELCNSCHNTEGHYYTFTGHAREGLLCTDCHLRVNNSPDAGMMGHG
                     ARNHSFKVDLQSCNDCHLGDMHSEADEAMANASIEQKTDVACYPVNAPEPVEFTPARQ
                     APIAYVSPTPTGPSPLVYVLPAGIGLVFGAMMAPWLDRLSKKRRENGEKGDE"
     gene            1361..2179
                     /locus_tag="PLC06_12410"
     CDS             1361..2179
                     /locus_tag="PLC06_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011007829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRO91295.1"
                     /translation="MSSKIDRRDFLKLGIATTATAAIGIGMSNTGLIPLRMAAPQAPA
                     TTKGPENPPHKWVMVIDQSKCVGCDLCLAACHAYNDTPPNISWSRVEEVQPLEDGQRV
                     FRPMPCQHCQDAPCVDVCPVGATYHRYDGLVMMDYDKCIGCRYCQLSCPYGARFFNWE
                     KFIGPNPDVPAAGTPEVERRPRGVVEKCTFCVHRIDRGLSAGLTPGIDPDATPACVVA
                     CTYGARLFGDLNDPDSPVSVALRNNPDAYRLREELGTEPRVYYLPAKSKEGSAS"
     gene            2176..>2780
                     /gene="nrfD"
                     /locus_tag="PLC06_12415"
     CDS             2176..>2780
                     /gene="nrfD"
                     /locus_tag="PLC06_12415"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRO91296.1"
                     /translation="MKQHITKERLTHLMHEYVAPASREPVVRWMAFLSVFMVVGVGAA
                     LMVFIKGLVITNLTDLVPWGLWISIDLSAIALAAGAFSVSAIAYLLRKKELQPVAKTA
                     VFVGFVGYSIAMMMLLLDIGRPDRFWHGFVFWNIHSPLWEVTMCVGLYFSVLMMEVLP
                     IIGHWHPVEHNFPRLSARMTGLHRFTPVLALIGLCLSTLHQS"
     assembly_gap    2781..2880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2881..3300
                     /locus_tag="PLC06_12420"
     CDS             <2881..3300
                     /locus_tag="PLC06_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91297.1"
                     /translation="EALYILTRGRLAFNFWWLEIILGMVVPAIILLIPQFRRNEQLRM
                     VALALVAVGVVAFRWNTNLVGQLVVFGTVPGSDVPLFTHYTPSLVEILTGTGVIAYGL
                     LAITFGVRYLHVIDHGEGVEAPAEASVSAAMVPSSSR"
     gene            3514..4167
                     /locus_tag="PLC06_12425"
     CDS             3514..4167
                     /locus_tag="PLC06_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO91298.1"
                     /translation="MSPLEDLLERLDATRESTLIALEMLSDEALVAPGVIGRWSIADL
                     LSILTAWDAEVVTGLLRLQQGKPPEKLLAAVANPNLYNAARYQDAQGRDLEVIFEDFQ
                     GARLHLEEWLSELSEKALSDPKHYKTLGGVSLGKIIAKATYEHEARYMPFLVSYAEAW
                     ESEAGFDEPGADPEDAETEPIVTLADIILIDAVYADDLLSPNGDGPEETHDDSLDDD"
     gene            4145..>4918
                     /locus_tag="PLC06_12430"
     CDS             4145..>4918
                     /locus_tag="PLC06_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HRO91299.1"
                     /translation="MTHLTMIEPVSLANAYEAAHSAAVVVHHAERGMIHLTGNTRLDL
                     INRMSTQAVGKLQSGEGAATVLTTNIGRIIDRPILYATSDSAYLLTGEGHAPALTRFF
                     MRNIFFNDDVQVRDISGETVVLGVYGPRAAEMLVAVGFAEVDIPLHHWRRGEIGGAQA
                     YIHRTDPIAGGGCFVIATAADREALLATLSAAGLPLIDDATYDYLRIEAGLPRFGHEL
                     TLDYIPLEANLWADVSFNKGCYVGQEIIARMESRGKLAKR"
BASE COUNT          915 a         1570 c         1416 g          917 t
ORIGIN      
        1 tttcccggtc gctatttttc cgggcgcgat agttaactga tgtctctgtt tgtagcggga
       61 atcgatcttc ctgctcactc tcagaatacg cgatccggtg catgggtgta aatcgggcga
      121 acaggccatt taatgctgac gcaaatcccc cagagtagcg cagacattcc tgtctgcgcc
      181 tcttcgcagg caggaatgtc tgtgctacag gctgcgcata tggggaaatc ccccatacgg
      241 gcatgtgaca gaaattgggc gattgcccaa tttacccgcc tccgtccccc ccttatactg
      301 ttcatagttg ctcaatttat tgaccatccg cccggcctcc cggaccggta acggaggagg
      361 acttgaatct gatgaaaagc ctacagaaca cacggattat cctcggcgcg ctgcttggcg
      421 cgctgatgct gatcctggcg ttcgcggcca tccccaccgc ttcggcagcg ccactgcggc
      481 aagcccccac acccgagcca aacgcgcagg agtgtgtcgg ctgccatgaa ggattacgcg
      541 gctattggga ccagagcgcc cacggctatg ccctgacgga cgccaatttc cagaaggcat
      601 ggaccgagca gggcagcccg aacgaatgtc tggcctgcca caccaccggc tatgaccccg
      661 agacaggcag ctacaccggg ggcggagtcg gctgcctttc ctgccactcg ccggttccca
      721 gcaatcaccc cgaggcctat atgccgacag atgtgtcgtc acgcctgtgc ggtaattgtc
      781 atatcgacac cttcgccgag tgggagatta gcgagcacgg cgagcagggc atgacctgta
      841 atcaatgcca taacccgcac acagccaagc ttcgcgtcgg cacatcgcag gagttgtgca
      901 atagctgcca caacaccgag ggccactact acaccttcac cggccacgcc cgcgaggggc
      961 tgctctgcac cgactgccac ttgcgcgtca ataattcgcc cgatgcgggg atgatgggcc
     1021 acggcgcgcg caaccattca ttcaaggttg acctgcaatc ctgcaacgat tgccatctcg
     1081 gcgatatgca ctccgaggcc gacgaggcga tggccaacgc cagcatcgaa cagaaaaccg
     1141 acgtggcctg ctaccccgtg aacgcgcccg aaccggtcga attcacccct gcccgacagg
     1201 ccccaatcgc ctacgtcagc cccacgccca ccggccccag cccgctggtc tacgttttgc
     1261 cggccggaat cggcctggta ttcggcgcga tgatggcccc atggctcgat cgtctgtcga
     1321 agaagcgccg cgaaaacggc gagaagggag acgagtaacc atgtcgtcga agatcgatcg
     1381 ccgcgacttt ttgaagctgg ggattgccac caccgccacg gccgctatcg gcatcggtat
     1441 gagcaatacc ggcctaatcc cgctgcggat ggccgcaccc caggcacccg ccacgaccaa
     1501 ggggccggaa aacccgcccc acaaatgggt gatggtcatc gaccaatcca aatgcgtcgg
     1561 ctgcgacttg tgcctggcgg cctgccatgc ctacaacgac acgccgccca acatctcctg
     1621 gtcccgcgtt gaggaagttc agccactgga agacggccaa cgggtatttc gccccatgcc
     1681 gtgccagcat tgccaggacg cgccctgtgt cgatgtttgc cccgtcggcg cgacttatca
     1741 tcgctatgat ggcctggtca tgatggacta tgacaaatgc atcggctgcc gctactgcca
     1801 actgtcctgt ccttatggcg cgcgcttctt caactgggag aagttcatcg gcccgaaccc
     1861 cgacgtgccc gccgccggca cccccgaggt ggagcgacgg ccgcgcggtg tggtcgagaa
     1921 atgcaccttc tgtgtgcatc gcatcgaccg cggcctgtcg gccgggttga cgcccggcat
     1981 cgaccccgac gcgacgcccg cttgcgtcgt cgcctgcacc tatggggcgc gcctgttcgg
     2041 cgacctcaac gacccggatt caccggtcag cgtcgccctg cgcaataacc ccgatgccta
     2101 tcgtctgcgc gaggagctgg gtaccgagcc gcgcgtctac tacctgccgg ccaaatcgaa
     2161 ggaagggagc gcatcatgaa acagcacatc acgaaagagc gcctcacgca tctgatgcac
     2221 gaatacgtcg cgcccgcttc gcgggagccg gtcgtccgct ggatggcctt cctgagcgtg
     2281 ttcatggttg tcggcgtcgg cgcggcgctg atggtgttca tcaaggggct ggtcatcacg
     2341 aacctgaccg acctggtgcc gtgggggctg tggatctcca tcgacctgtc ggccattgcc
     2401 ctggccgccg gcgcatttag cgtctcggcc atcgcctatc tgctgcgcaa gaaagaactg
     2461 caaccggtgg ccaagacggc cgtcttcgtc ggtttcgtcg gctacagcat cgccatgatg
     2521 atgctcctgc tcgacatcgg ccgcccggat cgcttctggc acggctttgt gttctggaat
     2581 atccactcgc cactgtggga agtgacgatg tgcgtcggcc tctacttctc ggtgctgatg
     2641 atggaagtgc tgcccatcat cggccattgg catccggtgg agcataactt cccgcgtctc
     2701 tcggcgcgga tgaccggcct gcaccgcttc acgcccgtgc tggcgctcat cggcctgtgc
     2761 ctttccacgc tgcaccagtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 gaggcgctct atatcctgac gcgcggccga ttggccttca atttctggtg gctggagatc
     2941 atcctgggga tggttgtgcc ggccatcatc ctgctgattc cgcaattccg ccgcaacgaa
     3001 caattgcgaa tggtcgctct ggcgctggtc gccgtcggcg ttgtggcttt tcgctggaac
     3061 accaacctgg tcggccaact ggtcgtcttc ggtacggttc cggggagtga tgtaccttta
     3121 ttcactcatt acacgccatc acttgttgag attctgacgg gaaccggcgt gatcgcctac
     3181 ggcctgctgg cgatcacgtt cggcgtcaga tacctccacg tgattgatca tggagaaggg
     3241 gtggaagcgc cagccgaagc ctctgtgtcc gccgccatgg taccctcctc ctcccgttga
     3301 gcaactcagg aaaagggtgg gctaagaaag cgccgcatcg ggcaaccggt gcggcgcttt
     3361 tgatttcgga ccgtcgaccg ctgacggacc cgtcgaccgc tgacggacgg catcgcaaga
     3421 gcagatcatt actgacccac tgacccactg acctccctta cgacagtcgc ccgcctgcgg
     3481 tcagtcgtca tcttatgcgc tataatccaa ccgatgtcac ctcttgaaga tttactcgaa
     3541 agactggacg ctacccgcga gagtacgctc atcgcgctgg agatgctgtc cgatgaggcg
     3601 ctggtcgcgc ccggcgtcat cggccgctgg tcaatagccg atttgctcag catactgacc
     3661 gcctgggacg ccgaagtcgt caccggcctg ctgcgcctgc agcaaggcaa accgccggag
     3721 aagctgctgg cggccgtggc taaccccaac ctctacaacg ccgcccgcta ccaggacgcc
     3781 cagggccgcg atctggaagt catcttcgaa gatttccagg gcgcgcgcct gcacctggaa
     3841 gagtggctaa gcgagctttc ggagaaagca ttatccgatc cgaaacatta caagactctg
     3901 ggcggggtgt ctctgggcaa gatcatcgcc aaggccacct atgagcacga ggcgcgctat
     3961 atgccgttcc tcgtctccta tgccgaggcg tgggagtcgg aggcgggatt cgacgagccg
     4021 ggcgcagacc cggaagatgc ggaaaccgaa ccaatagtca ccctggccga tatcatcctg
     4081 atcgatgccg tctacgccga tgacttgctg tcgcccaatg gcgatggccc ggaagaaaca
     4141 cacgatgact cacttgacga tgattgagcc ggtgtccctg gcaaatgcgt atgaggcggc
     4201 gcactcggcc gcggtggtcg tccaccatgc cgagcgcggc atgatccacc tcaccggcaa
     4261 cacgcgcctc gacctgatta accgcatgtc gacgcaggcc gtcggcaagc tgcaaagcgg
     4321 cgagggggcg gccacggtgc tgacgaccaa catcggccgc atcatcgatc ggcccatcct
     4381 ctatgccacg agcgacagcg cttatttgct gaccggcgag ggacacgccc ccgcgctgac
     4441 ccgctttttc atgcgcaata tcttcttcaa cgacgacgtg caggtgcgcg acatcagtgg
     4501 cgagacggtc gttctcggcg tctacgggcc gcgcgcggcc gagatgctgg tcgcagtggg
     4561 gtttgccgaa gtcgatatac cgctccacca ctggcggcga ggcgaaatcg gcggcgcgca
     4621 agcctatatc caccgcaccg accccatcgc cggcggcggc tgcttcgtca tcgccacggc
     4681 cgccgatcgg gaggctctgc tggcgacgct atcggccgcc gggttgcctc tgatcgacga
     4741 cgcgacctac gactatttgc gtatcgaggc cgggctgccc cgtttcggcc acgagttgac
     4801 cctggactac atcccgctgg aggccaacct gtgggcagat gtatctttca acaaggggtg
     4861 ttacgtggga caggagatca tcgcccggat ggagagccgc ggcaagctgg ccaagcgg
//
LOCUS       DAOMQG010000257         4913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7213_length_4913_cov_11.025319, whole genome shotgun sequence.
ACCESSION   DAOMQG010000257 DAOMQG010000000
VERSION     DAOMQG010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7213_length_4913_cov_11.025319"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLC06_12435"
     CDS             complement(<1..296)
                     /locus_tag="PLC06_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRO91300.1"
                     /translation="MSILVDKNTRLIVQGITGREGTFHTNQMAAFGTNVVAGVVPGKG
                     GQYFEGENGRVPIFDSVRGAAEATGGNTSIIFVPAKFAPEAMYEAADAGLPLVI"
     gene            complement(293..1453)
                     /gene="sucC"
                     /locus_tag="PLC06_12440"
     CDS             complement(293..1453)
                     /gene="sucC"
                     /locus_tag="PLC06_12440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRO91301.1"
                     /translation="MNLHEFQAKRLFAEHGIPIPHGDVAYTPAEARKITQDLGGRAVI
                     KAQVLTGGRGKAGGVKVAQSPDEAEANAKAILGMKIKGLTVNKVLVDELAPGIRQELY
                     LAILIDRSKRQPMIMASAAGGMDIEEVADKTPEKIHLVHIDPIIGVHSFQTTYLANKM
                     ELPREVWGDFYKIVSGLYECFKTNDASLTEINPLIITGEGKLMALDGKMSIDDSALSR
                     QPRLAEMRDVEEEPPAEAEARRTGITFIQLDGNIGCMVNGAGLAMATMDIIKLYGGEP
                     ANFLDIGGGARADQVATALRLILSDTKVEAVLFNIFGGITRGDEVARGILSALEQIDT
                     NVPMVVRLAGTNAQEGLALLAEADMMTAATLSEAAQKAVAVAEASRGVEKAS"
     gene            complement(1644..2138)
                     /locus_tag="PLC06_12445"
     CDS             complement(1644..2138)
                     /locus_tag="PLC06_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRO91302.1"
                     /translation="MSALADLFVSPAPVIFVLDGVEDPYNFGQAVRSIYAAGIDGLIV
                     PTRSWLSAAATVIRASAGATEHMPTATAATADAVAMAQSRRIPIGVATTDKARPMGEF
                     DLTGPLLLLIGGEKRGVARAVEEVADWRVSIPYGRETGYALGTAAAAAVLSFEVLRQR
                     QAKG"
     gene            complement(2500..3852)
                     /locus_tag="PLC06_12450"
     CDS             complement(2500..3852)
                     /locus_tag="PLC06_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRO91303.1"
                     /translation="MAKKQNTTPSDEAQERQSRKEILIARKNERQLRTIRIAVIAIFA
                     LIALVIGVALVNELIITPGRAVATVNDTPITLREWEERVKFERAQRIIYLDNQLEAFG
                     GDVGIVQQFAGSIINELFEPQTMGQNAINSMANEVIMCEALKERGIEISDADIQARIG
                     ESYGFYGDGISPTQQPSPAATIQPTPSLTPIPTAVITEVVPTLTPFPTPTLGPTSTPF
                     PTATPVSEAEFQTQFSDFMAQLTGLGVNEATYRAAVRAQLCSERLTEVLTEERNISRM
                     AEQASIFVIQATTEESANETQALVKSEGFLTAWNTISSRPDDPEATEQPETNAFELLW
                     RTQQSLASSIGTDIATAAFTLAIDTPSDIITVDNSDGTSSYYLIMVSGREERELSASE
                     FQTRQSEALQAFVDEQLTGNLQINDMWRGRVPTLPALDPKFLAAPTPTPAAAAPTVAP
                     "
     gene            complement(3880..4119)
                     /gene="rpsR"
                     /locus_tag="PLC06_12455"
     CDS             complement(3880..4119)
                     /gene="rpsR"
                     /locus_tag="PLC06_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRO91304.1"
                     /translation="MQATNRPNRRFRPTKRVCQFCVEKTEIDYKQVDLLNRYVNEFGR
                     MRPRRQTGTCAKHQRHLAREIKRARHIALLPFVAD"
     gene            complement(4133..4471)
                     /gene="ssb"
                     /locus_tag="PLC06_12460"
     CDS             complement(4133..4471)
                     /gene="ssb"
                     /locus_tag="PLC06_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRO91305.1"
                     /translation="MTSSKGLNKVMVIGWLEDAPEVRQTPGGRAVASFSVATSRSWTT
                     TEGEKHEEKEWFNVIAWGPLAETCRDRLAKGQQVYIEGRLQTRGWEDTLGRKHFRTEV
                     VAQDLIVLTE"
     gene            complement(4494..4787)
                     /gene="rpsF"
                     /locus_tag="PLC06_12465"
     CDS             complement(4494..4787)
                     /gene="rpsF"
                     /locus_tag="PLC06_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRO91306.1"
                     /translation="MREYEVTVVIQPQLEEAERGQLIERLSNLLVPGSKEDGALTANH
                     WGMRNLAYPIKKFQEGYYVLYEAQIDPTRIKDIERSMQFNEDVLRYLVVRKGE"
BASE COUNT          976 a         1480 c         1395 g         1062 t
ORIGIN      
        1 cagatgacca gcggcaagcc tgcgtcggcc gcctcataca tggcttcggg agcgaacttg
       61 gcgggtacaa agatgatgga cgtgttccca ccggtcgctt cggccgcccc tcggacggaa
      121 tcaaagatcg gcactcggcc gttttcgccc tcgaagtatt ggccaccctt gccgggcacg
      181 acgccggcga ctacattggt gccgaaggcg gccatctgat tggtgtggaa cgtgccttcg
      241 cggccggtga taccctggac aattaaccgc gtattcttat cgactagaat gctcatgatg
      301 ctttctcaac tccccgcgat gcctcggcca ccgcaaccgc cttctgtgcc gcctcggaca
      361 gcgtcgccgc cgtcatcatg tcggcctcgg ccaacagggc cagtccttcc tgggcattgg
      421 tgcccgccag acgaaccacc atcggcacgt tggtgtcaat ctgttccaac gcggacagga
      481 taccgcgcgc cacttcatcg ccacgggtga tgccgccgaa aatattaaac agcaccgcct
      541 caaccttcgt atcggacagg atgaggcgca acgccgtagc cacctgatcc gcccgtgcgc
      601 cgccgccgat gtcgaggaag ttggccggtt cgccgccata gagcttgatg atatccatcg
      661 tcgccatcgc caggcccgcg ccgttgacca tgcacccgat gttgccgtcc aactgaatga
      721 aggtgatacc ggtgcgacgc gcctcagcct cggccggcgg ttcctcttcc acatcgcgca
      781 tctcggccaa acgcggctgg cgagacaggg cgctgtcgtc gatcgacatc ttgccgtcaa
      841 gagccatcaa ctttccctcg ccggtgataa tcaacgggtt gatctcggtc agcgaggcat
      901 cgttggtctt gaagcattcg tacaagccgg acacgatctt gtaaaagtct ccccaaacct
      961 cgcgcggcaa ctccattttg ttggcgagat aggtcgtctg gaagctgtgg acgccgatga
     1021 tgggatcgat atggacgaga tggatcttct ccggtgtctt gtcggccacc tcttcgatat
     1081 ccatgccacc cgcggccgag gccatgatca tcggctgccg cttgctgcgg tcgatcaaga
     1141 tcgccagata caactcctga cgaataccag gggccaactc atcgaccaac accttgttga
     1201 ccgtcagtcc cttgatcttc atgcccagga tcgccttggc attggcctca gcctcatcgg
     1261 gggattgagc aaccttgacg ccgcccgcct tgccacggcc gccagtcagg acctgggctt
     1321 taatgacggc gcggccgccc aggtcttgcg taatcttccg ggcctctgcc ggcgtgtagg
     1381 cgacatctcc gtgcggaata ggaatcccgt gttcggcaaa cagccgcttg gcctgaaact
     1441 catgtaggtt cacagcactc tccttctatc cattggaatc accttctagg cccgtggcgc
     1501 aatagcacag cgcaattgat tggccttcgt tcgcgtcggc gggcaaaatg aaaggcgaac
     1561 agaaacgtcc attcgcctct gaatataact gatttaacga gcggtagcag tatagcacag
     1621 cggtatttga cgcgcgaaat cgatcaacct ttcgcctggc gttggcgcaa gacctcgaac
     1681 gacaggacag cggctgcggc ggccgtcccc aaagcataac ctgtttcccg gccatacggg
     1741 atgctcacac gccaatcggc cacctcttcc acggcgcgcg ccacgccccg tttctcgccg
     1801 ccgatcagca gcaacagcgg cccggtcaga tcgaactcgc ccatgggacg agccttgtcg
     1861 gtcgtggcta caccaatcgg tatccgccgt gactgggcca tggcaacagc atccgcagtc
     1921 gccgccgtgg cagtcggcat atgctccgtg gcgccggccg aggctcgaat gaccgtcgcc
     1981 gcggccgaca gccaactgcg ggtgggcacg atcaggccat ctattccggc cgcgtatatt
     2041 gagcgaaccg cctggccaaa gttgtaaggg tcttcgacgc catccagaac gaaaatgaca
     2101 ggcgcgggtg acacaaacag gtcagcgagc gcgctcattc gtcgcggccc aactctggca
     2161 ataaccccac catgtgactt attgggagcg tgctcatcga ttaccgccgc aggaacgcga
     2221 gacacagtca tccccgtctg ccgtgccaga tgttgaagcc gagccacagg tccatcaaac
     2281 cgatcccggt tgatcaggag ttccaccatt tcgcggcttt gagcctgaag cacagcctcg
     2341 acagccaaga ccccttcaat ccattgatac tcttccataa ggtcaggcgc tcactttgct
     2401 atagccggaa acaaatgaaa gcaaaaggcc agataaagct atctggcctt ttgaatgtta
     2461 tctaatcacc ggcggtcact cgccaacgct tgttcagagc tagggggcga cggtcggagc
     2521 tgcagcagcg ggtgtcggcg tcggcgcggc caggaatttg ggatccaggg ccggcagcgt
     2581 gggcacacga ccacgccaca tgtcgttaat ctgcaggttg ccggtgagtt gttcatcgac
     2641 aaacgcctgg agcgcttccg actggcgcgt ctggaattca gaggcgctca actctctctc
     2701 ttcacgaccg ctaaccatga tcagatagta gctcgacgtg ccgtcgctat tatcaaccgt
     2761 gataatatcg ctgggcgtgt cgatagccag ggtgaaggcc gctgtggcta tatcggtgcc
     2821 gatgctgctt gcgaggctct gctgtgtgcg ccagagcaat tcgaaggcat tggtctcagg
     2881 ctgttccgtc gcctcgggat cgtccggccg gctggaaatg gtgttccacg ccgtcaggaa
     2941 gccctcggac ttgacgagcg cctgcgtttc attggcagac tcttcggtcg tagcctgaat
     3001 tacgaagatg ctggcctgtt cggccatgcg cgaaatgttc cgctcttcgg tcaacacctc
     3061 ggtcagccgc tcgctgcaca attgtgcgcg gacagcagcg cgatacgtag cctcattcac
     3121 gcccaggccg gtcagttgtg ccatgaaatc gctaaactgc gtctggaact cggcctcgga
     3181 tacaggcgtg gccgtgggga acggggtaga ggtcgggccc agagttggcg tcgggaacgg
     3241 cgtcaacgtg ggaacgactt cggtaatgac cgccgtcggg ataggcgtca gcgatggtgt
     3301 gggctggatc gtggcggccg gacttggctg ctgggtcggc gaaatgccgt cgccataaaa
     3361 gccataggat tcgccgatcc gggcctgaat atcggcgtcg gagatctcaa tgccacgctc
     3421 tttgagcgct tcacacatga tcacttcgtt ggccatcgaa ttgatggcgt tctgccccat
     3481 ggtctgcggc tcgaatagtt cgttgatgat gctcccggca aactgctgga cgataccaac
     3541 atcgccgccg aatgcttcaa gctgattatc aaggtagata atccgctgag cgcgctcaaa
     3601 tttgacgcgc tcttcccact cgcgcagggt tattggcgtg tcattgaccg tggccacggc
     3661 ccgaccggga gtgatgatta gttcattaac caaagcaacg ccgatcacta aagcgatgag
     3721 cgcaaagata gcgatcaccg caatgcgaat ggtgcgcaac tggcgctcat tcttgcgggc
     3781 gatcaggatc tctttgcgcg actgtcgctc ttgggcttcg tcgcttggag tcgtgttttg
     3841 tttcttagcc atgtaacatt acaaggaatg ccaggaggct tagtcagcca cgaacggcaa
     3901 caaggcgatg tgacgggcac gcttgatctc gcgggccaga tggcgctggt gcttggcgca
     3961 ggtgccggtc tggcggcgcg gccgcatgcg gccaaattca ttgacatagc ggttcagcag
     4021 atcgacctgc ttgtaatcaa tttcggtctt ttcgacacag aactggcata cgcgcttggt
     4081 agggcgaaaa cgccggtttg gacggttagt agcttgcatc tctcaatcct ctttactcag
     4141 tgagcacaat caaatcctgg gcaacaactt ccgttcggaa gtgcttgcgg ccgagggtgt
     4201 cctcccagcc gcgggtctgt agacgaccct cgatgtatac ttgctgacct ttggcgagtc
     4261 ggtccctgca cgtctccgcc agggggcccc aggcgatgac gttgaaccat tctttttcct
     4321 cgtgcttctc gccttcggtc gtcgtccaac tgcgagaggt ggcgacgctg aacgaagcca
     4381 cggcgcggcc gccgggggtc tgtcgcacct ctggtgcgtc ttccagccaa ccaatcacca
     4441 tgactttgtt caagcctttg cttgaagtca tcgcctctcg ccccgcttaa aagttactcg
     4501 cccttgcgca cgacaagata acgaagaaca tcttcgttaa actgcatgct gcgctcgata
     4561 tctttaatgc gggtcgggtc tatttgcgcc tcatagagga cgtagtagcc ttcctggaac
     4621 ttcttgatgg ggtaagccag gttccgcatt ccccaatggt tggcggtcag agcgccgtct
     4681 tccttggagc cgggaaccag caaattgctg agacgctcga tcaattggcc gcgttcagct
     4741 tcttccaact gtggttggat gacaacagtt acttcgtatt cacgcacgtt gcattactcc
     4801 tttccttgga atttactcta gcgagcattg cccccggtca cttgccggga gcggaagccg
     4861 gggaatggtc gtgctttacc attgccgggc aacgaattcg ctattatagc ctc
//
LOCUS       DAOMQG010000258         4894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7243_length_4894_cov_5.371358, whole genome shotgun sequence.
ACCESSION   DAOMQG010000258 DAOMQG010000000
VERSION     DAOMQG010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7243_length_4894_cov_5.371358"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLC06_12470"
     CDS             complement(<1..249)
                     /locus_tag="PLC06_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91307.1"
                     /translation="MATGTGTRAAPPYAVTAAEIVNDIRVALDDTAAAAWTDAELLGF
                     LAEAVREYSQHLPRVGEAALTADTGQRRYALPWDAAAVI"
     gene            complement(358..1329)
                     /locus_tag="PLC06_12475"
     CDS             complement(358..1329)
                     /locus_tag="PLC06_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91308.1"
                     /translation="MFLKQSTAATIVLGPFVDDTDGKTAETALTISQADVRLSKNGGA
                     YAQKNDTGSASHMEAGNYSVSLNTTDTNTLGRLRISVHESGALPVWIDLMVMPSNVWD
                     SFFSTDRLQVHAAEIDNGLITAAAIATDAIDADALATNALNEIADAVWDEAISGHSTA
                     GSTGEALSDASAGSATAGQIADAVWDEALSGHLVAGSTGAALDDAGGAAADPWNTTLP
                     AAYSSNTAGWILGTRLDAKVSAITGNSPGAGAVEFTYTLTEPDLVTPIPDADVWVTTD
                     SAGATVVASGRTNQYGIITFYLDAGTVYLWRQKSGYNFTNPDTETVP"
     gene            complement(1330..1539)
                     /locus_tag="PLC06_12480"
     CDS             complement(1330..1539)
                     /locus_tag="PLC06_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91309.1"
                     /translation="MATDIGTGTGTKTTGPTPAQTAEQSIIIIRDFKQGEIVRVTFLT
                     PDQAKALPTIPPDALQIAIAPAGGL"
     gene            complement(1524..2114)
                     /locus_tag="PLC06_12485"
     CDS             complement(1524..2114)
                     /locus_tag="PLC06_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91310.1"
                     /translation="MATYSASTAASTDDGQEAAGTVSLGGSNINANSASQYIGLRFLN
                     VTIPNAATIDAASLDLYFVSTSFDDPDVTIYAEATDDAATFTTTTNDMSGRASTTATV
                     NWTASSLGTGIKTTPSIASIIQEIVNRAGWVSGNDIVLLLKGNGSSSFIRVRAYDDGG
                     GSYPTLNIDYTVGGGGGQPPRTMHQFRTRRNQWLQI"
     gene            complement(2267..2428)
                     /locus_tag="PLC06_12490"
     CDS             complement(2267..2428)
                     /locus_tag="PLC06_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91311.1"
                     /translation="MRELQAALREMGLSWADVWALSLDRRAGTLTVVTADGRKLNGRL
                     NERRMTNDE"
     gene            complement(2521..>4894)
                     /locus_tag="PLC06_12495"
     CDS             complement(2521..>4894)
                     /locus_tag="PLC06_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91312.1"
                     /translation="GREGEKGGRGEGEKGRRGEREKGGKGEGERADVGVSLVFYPRLA
                     ADGRTVVGMAMVESADVVMFPASAGSRIVGEITNDESGMTNEERRAANYVLRTTNDER
                     RMTGDKGDGAGYGAAARSVALGLWAAGGAEQEEGDIASHSQSANPQPNPTKAALPTRA
                     AAPAHSMASHSLLNNQQLHSAQQTEPTSPNPAGPTNCGTGRRLASSPLAQAASFSGAA
                     AIRRGDASCSTGRWLASPPLAQAASFSGTAAIRRGDASCGASCRLASSPLAQPASFSG
                     AAAVRRGDASCSASCQLAQQGNPHLSTQTNQQENNSMTQQTEGTLAPRVALVMTTDNR
                     PSSPDSGQSVVGRPADPWLDTLRTTTAEAVIRASGLPPVVQERLSAARYETTEALNSA
                     VAGARAELAALNEAGVVRLDGLRPFAAARLRDGLDEARELMGFFFGAAGAATPPGNMR
                     SFADLYVALTGDAHFRGVFDGGRVLFSGATTATLPNLAVDAMNRVIAEQFAALEHYRW
                     YERVASVAPNDGRLHAMKWLTLGGIGDLPAVSEGAAYTELSVADAKESASFVKRGGYV
                     GITREMIKNGDIMQMQAIPRALAAAAVRTRSAAVGALFTANSGVGPTLAQDSKALFHL
                     DHANLATTALGTDAAAWRAARSECFQQPELGGGKPLGIFPRYLLVPAELYDTALSILG
                     YGEGMPTVYTPEAQGRGFADPRPIPLVVPEWTDATDWAYIVDPALFPVIQMSYSQAPG
                     GRSHPIPELFAVVSETSGLMFSNDVLPIKVRDEFALGVNGPRGIGKRNVA"
BASE COUNT          747 a         1455 c         1711 g          981 t
ORIGIN      
        1 gatgacggcc gcggcatccc acggcagggc gtagcggcgt tggccggtgt cggccgtcag
       61 cgccgcctcg cccacacgcg gcaggtgttg ggaatactcg cgcaccgcct cggccagaaa
      121 gcccagcagc tcggcgtctg tccatgcggc cgcggcggtg tcatccagcg cgacgcgaat
      181 atcgttcacg atctcggccg ccgtcaccgc ataaggcggc gcggctctcg ttcctgttcc
      241 tgttgccatg attgtctcct gaacgtgtga aggggggaag gggagaaagg gtgaaggggg
      301 gaaagggaga aagggtgatc agagcacccc tgctcccccg ctcccccgct ccccttctca
      361 aggaactgtc tccgtatcgg ggttggtgaa gttgtagccg gacttttggc gccagaggta
      421 gacggttccg gcgtcgaggt agaaggtgat gatgccgtat tggttggttc ggccgctggc
      481 gacgacagtc gctccggcgc tgtcggttgt gacccagacg tcggcgtcgg ggatgggggt
      541 gacgaggtcg ggttcggtga gggtgtaggt gaactcgacg gctccggcgc ctgggctgtt
      601 gccggtgatg gcgctgactt tggcgtcgag gcgggttccg agaatccagc cggcggtatt
      661 ggaactgtag gcggcgggga gtgtggtgtt ccaggggtcg gcggcggcgc cgccggcgtc
      721 gtcgagtgcg gcgccggtgg aaccggcgac gaggtggccg gagagggctt cgtcccagac
      781 ggcgtcggca atttgtcctg ctgtggcgct gccggcggat gcgtcggaga gcgcctcgcc
      841 ggtggaaccg gcggtgctgt gtccgctgat tgcttcgtcc cagacggcgt cggctatttc
      901 gttgagtgcg ttggtggcga gtgcgtcggc gtcgatggcg tcggttgcta tggcggctgc
      961 ggtgatgagg ccgttgtcta tttcggccgc gtgaacctgg aggcggtcgg tgctgaagaa
     1021 tgaatcccag acgttggaag gcatgaccat gaggtcgatc cagacaggga gggcgccgga
     1081 ttcgtgaacg ctgatgcgga ggcggccgag ggtgttggtg tcggtggtgt tgaggcttac
     1141 gctgtagttt ccggcttcca tgtgggaggc gctgccggtg tcgtttttct gggcgtaagc
     1201 gccgccgttt ttgctgaggc ggacgtcggc ctggctgatg gtgagggcgg tttcggcggt
     1261 tttgccgtcg gtgtcatcga cgaaggggcc gaggacgata gtcgcggcgg tggattgctt
     1321 tagaaacatc tagaggcctc cggcgggggc aatggctatc tgcagggcgt cgggcgggat
     1381 ggtgggcagg gctttagcct ggtctggcgt taggaaggtg acgcggacaa tctcaccctg
     1441 tttgaagtca cgaatgatta tgatggattg ttcggccgtt tgtgcggggg tcgggcctgt
     1501 tgttttggtg cctgttcctg tgcctatatc tgtagccatt ggtttcgtct cgttctgaac
     1561 tggtgcatgg tgcggggcgg ctggccgcct ccgcctccga cggtgtagtc gatgttaagt
     1621 gttgggtagg agccgccgcc gtcgtcgtag gcgcggacgc ggatgaagga tgatgagccg
     1681 ttgcctttga ggaggaggac gatgtcgttg ccggataccc agccggcgcg gttgacgatt
     1741 tcttgtatga tgctggcgat ggatggtgtt gttttgattc cggttccgag actggacgca
     1801 gtccagttga cggtggcggt ggttgaggcg cggccgctca tgtcgttggt ggtggtggtg
     1861 aatgtggcgg cgtcgtctgt ggcttcagcg tagatagtta cgtcggggtc gtcgaaggaa
     1921 gttgagacaa agtagaggtc gaggctggcg gcgtcgatgg tggcggcgtt ggggatggtg
     1981 acgttgagga agcgcagtcc gatgtattgg gaggcgctgt tggcgttgat attggagcca
     2041 ccgagagaga cggttccggc tgcttcttgc ccgtcgtcgg tggatgcggc ggtggatgcg
     2101 gagtaggtgg ccataattaa ttacgaatta cgaattacga attacgaatg aagatagtgg
     2161 gtcagtgggt cggtgggtcg gtgggtcagt gggtcagtag gtcgcaatcg ctctcgcaat
     2221 cgctttcgat cttcgattac gatagcgatc cggaaccggt cgttcgtcat tcgtcgttcg
     2281 tcattcgtcg ttcgttcagc cggccgttga gcttgcggcc gtcggccgtc acgacggtga
     2341 gcgtccccgc ccgccggtcg agcgacaacg cccacacgtc ggcccacgac agacccatct
     2401 cccgcaatgc tgcttgcaat tcgcgcataa aaccctccgg tagatacgag atacgagata
     2461 cgagatacga aagaagaacg catctctgaa ctcgtatctc gtatttcgta tttcgtatct
     2521 ctacgccacg ttccgcttgc cgataccgcg cggaccgttg acgccgagag cgaactcatc
     2581 gcgcaccttg atcggcagca catcattgct gaacatcagg ccgctggtct cgctgaccac
     2641 ggcgaacagc tccgggatag gatgcgaacg accgccaggg gcttgcgaat agctcatctg
     2701 gatcaccggg aacagggccg gatcgacgat gtaggcccag tcggtcgcgt cggtccattc
     2761 cggcaccacc agcgggatcg ggcgggggtc ggcgaagccg cgcccctgcg cttcgggcgt
     2821 gtagactgtg ggcatgccct cgccatagcc caggatgctg agcgcggtgt catagagttc
     2881 ggccgggacg agcagatagc gcgggaagat gcccaacggc ttgccgccgc ccagctccgg
     2941 ctgctggaaa cactcgctgc gggcggcgcg ccaggcggcc gcgtccgtgc ccagcgccgt
     3001 cgtcgccaga ttggcgtggt cgaggtggaa cagcgccttg ctgtcctggg ccagcgtcgg
     3061 ccccacgccg ctgttggcgg tgaagagcgc gccgacggcc gcgctgcggg tgcgcacggc
     3121 ggcggcggcc aatgcgcggg ggatggcttg catctgcatg atgtcgccgt tcttgatcat
     3181 ctcgcgggtg atgcccacat agccgccgcg cttcacgaac gatgccgact ccttcgcgtc
     3241 ggccacgctc agctcggtgt aggccgcgcc ctcgctgacc gccggcagat cgccaatgcc
     3301 gcccagcgtc agccacttca tggcgtgcag gcggccgtcg ttgggggcca cgctcgccac
     3361 ccgctcatac cagcggtaat gctccagcgc ggcgaactgc tcggcgatga cccggttcat
     3421 ggcgtccacg gccagattgg gcagcgtggc cgtcgtcgcc ccgctgaaca gcacgcggcc
     3481 gccgtcgaac acgccgcgga aatgggcgtc gccggtcagg gccacgtaga ggtcggcgaa
     3541 ggagcgcatg ttgcccggcg gggtggccgc gccggccgcg ccgaagaaga agcccatcag
     3601 ctcgcgcgcc tcgtccagcc cgtcgcgcag tcgcgccgcg gcgaacggcc gcaagccgtc
     3661 gagacgcacc acacccgcct cattcaacgc cgccagttcg gcgcgcgcgc cggccacggc
     3721 cgaattgagc gcctcggtcg tctcatagcg cgcggcgctc agccgctcct gcaccactgg
     3781 cggcagcccg gatgcgcgaa tgaccgcctc ggccgtggtg gttcgcaggg tatcaagcca
     3841 gggatcggcg ggccggccga cgacggattg accgctgtcg ggggatgacg gccgattgtc
     3901 ggttgtcatg accagcgcca cgcgcggcgc aagtgttcct tctgtttgtt gtgtcattga
     3961 gttgttctcc tgttggttgg tttgcgtaga taaatgggga ttcccctgtt gagcaagctg
     4021 acagcttgcg ctacaagagg cgtcgccgcg ccgaacggcg gccgctccag aaaacgatgc
     4081 gggttgcgcc aggggggaag aggcaagccg gcagcttgcg ccacaagagg catcaccgcg
     4141 ccgaatggcg gccgttccag aaaacgatgc ggcttgcgcc agggggggag aggcaagcca
     4201 gcggcctgtg ctacaagagg catcaccgcg ccgaatggcg gccgctccag aaaacgatgc
     4261 ggcttgcgcc aggggggaag aggcaagccg gcggcctgtg ccacaattgg tggggccggc
     4321 gggattgggg ctggtgggtt cggtttgctg ggcggaatga agttgctggt tatttaatag
     4381 gctatgcgaa gccatgctat gggccggggc tgctgcgcgg gtgggcaagg ctgcttttgt
     4441 gggatttggt tgcgggttag ccgattggct atgcgaagca atgtcgcctt cctcctgctc
     4501 tgccccaccc gccgcccaca gccccagcgc cacgctccgc gccgccgccc cataccccgc
     4561 cccgtcgccc ttatcgcccg tcattcgtcg ctcgtcgttc gtagttcgta gcacgtagtt
     4621 cgccgcccgc cgctcttcat tcgtcattcc tgattcgtca ttcgtaattt cccccacaat
     4681 ccggctcccc gcgctggccg gaaacatcac cacatcggcc gattccacca tcgccatccc
     4741 caccaccgtc cgcccgtcgg ccgccagccg cggatagaac accagactca cccccacgtc
     4801 cgcccgctcc ccctctccct ttcccccctt ctccctttcc ccccttctcc ccttctcccc
     4861 ctctcccctt ccccccttct ccccttctct cccc
//
LOCUS       DAOMQG010000259         4875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7276_length_4875_cov_5.096888, whole genome shotgun sequence.
ACCESSION   DAOMQG010000259 DAOMQG010000000
VERSION     DAOMQG010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7276_length_4875_cov_5.096888"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..752)
                     /locus_tag="PLC06_12500"
     CDS             complement(288..752)
                     /locus_tag="PLC06_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRO91313.1"
                     /translation="MSERLDYKNASPEAFKAMLGMEQQIHKSGLEKPLLELVKSRASQ
                     LNGCAWCLDMHTKDARACGETEQRLYLLPVWREASCYSDRERAALAWTEAVTLVAATQ
                     DVDDEVYEDAHRHFDEKELVDLTLAVIAINGWNRLNVAFRTPVGDYVSPYAK"
     gene            complement(775..1602)
                     /locus_tag="PLC06_12505"
     CDS             complement(775..1602)
                     /locus_tag="PLC06_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRO91314.1"
                     /translation="MDKHIQIQRAQRFRALHDRRRVLLLPNAWDAGSARIFAGLGFDA
                     VATTSAGVAWALGYPDGEGTPLDEVLAVVERIVRVTPLPVTVDFEAGYGDSPEAVAAN
                     VHRLIETGAAGINLEDGVRHEYLRDIDDAARRVAAARQAADEAGVPIVINARVDNWMV
                     GGDEDEDTRVAEALRRARAYLEAGADCIYPIGVSSPELIGRLCAAIDAPVNVGVRPGL
                     PDMAELGRLGVARVSAATRLATVAFSAACDAAKRMRAGGRFDGLDSSLAYGDIQRLF"
     gene            complement(1804..3069)
                     /locus_tag="PLC06_12510"
     CDS             complement(1804..3069)
                     /locus_tag="PLC06_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO91315.1"
                     /translation="MRKILAITWKDISVLLRDRTALLLAVAAPLALTLAMGLVTGGFG
                     DNAAPTIANIPVLIVDHDGGQFATTLIDIFTGDDLSEFVTAEQWDDEAAAIAAVGAGD
                     ATAAVIIPPGFSDSFLPDATGATPAPVALRVYGDPGRPIGASIVRSIAEEFTRRVQTG
                     VTTVQIAMTDLIMSGAISPEALPEIGRAMGQGLFNRDDPTGGSLIAIHNETAAAGGAA
                     DAPVGSLSFFAPGMALFFLMYAVTLGARTFLSERREGTLARMLAAPVSSAQVLSGKVG
                     AIFVSGVLQLGVLVLLTSLLFRLNWGSPPGVGLVITAAALAATGWALLLASTATNEGQ
                     VANLGMVLTLIFGMLGGNIVPTLQISSPVIDALARLTPNRWAMDGFNKLAYGEGAAAV
                     LPEVAALLVMAAALFVVAAAIFRRRQGNN"
     gene            complement(3081..4025)
                     /locus_tag="PLC06_12515"
     CDS             complement(3081..4025)
                     /locus_tag="PLC06_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO91316.1"
                     /translation="MTDPILIANDLRKSWGDLEAVKGISFSVARGEIFSLLGPNGAGK
                     TTTINMLSCLIEPTGGTAEIDGHAIPGDSLGVRRAIGVVPQEIALYESLSARQNLEFW
                     GRLYDMSGKALATRIDEVLALVELSDRARDKIKTFSGGMKRRINIAAGLLHRPKLLFM
                     DEPTVGIDPQSRRRILDMVKELRDDGMTVLYTTHYMEEAEELSDRVGIIDHGQLIAIG
                     TQAELTQVVGEGEVLRLHLAEEAADSAAELFAGLPGVVGAAAADHQIVLNMTNASEAL
                     AGVVGRANGANLNIRSIDIEEPNLESVFLHLTGRGLRD"
     gene            complement(4258..4800)
                     /locus_tag="PLC06_12520"
     CDS             complement(4258..4800)
                     /locus_tag="PLC06_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91317.1"
                     /translation="MAKKIRKYESGIRNEEAAEQLALPGMEEVRRDSCRATNDEMRDA
                     PPSPFPPAPLPPFSSSPLPPFPPAPLPPFSSSPPPGDHLYFPRPTAAEQRVLAATADE
                     PDLLPEVELVRLVLRRLLAHLDESAGELPVEEVRRLAALLFTGARTVALLLGKRAPAR
                     PETEAWLAEALREMMEDQEL"
BASE COUNT          795 a         1690 c         1568 g          822 t
ORIGIN      
        1 tcgacgaccg gcagcacaac gcggctgtcg ctcacgtcac tggagatgct ccggccgacg
       61 atcaccgtga agacgatggg cagcaccaga aagaagagca gctccgagcg gctctggaac
      121 cgtatcatcg tgtccttgcg catgatggcg atgagtttgt tgagcatgag acaccctccg
      181 gcaaacagga atcaggaatc aggaattggg cattcgtaat gttcatatct gtaggggcgg
      241 gtttcaaacc cgcccctacg cgccgacaac aaatcattcg cgccgcacta tttcgcatac
      301 gggctgacgt aatcacccac cggggtgcgg aaggccacgt tcagccgatt ccagccgttg
      361 atggcgatga ccgccagggt caaatcgacc agctctttct cgtcgaaatg gcggtgcgcg
      421 tcctcataga cctcatcgtc cacgtcctgc gtcgcggcca ccagggtgac cgcctcggtc
      481 caggccagcg cggcgcgctc gcggtcgctg tagcacgagg cctcgcgcca gaccggcagc
      541 agatacaggc gctgctccgt ctcgccgcag gcgcgcgcgt ccttcgtgtg catgtccaga
      601 caccaggcgc agccgttgag ctgcgaggcg cgggacttga ccagctccag cagcggcttt
      661 tccagcccgc tcttgtgaat ctgctgctcc atgccgagca tcgccttgaa agcctcgggc
      721 gacgcatttt tgtagtccaa tcgttcactc atgataaacc tccgagttaa aagttcaaaa
      781 caggcgctgg atatcgccat aggccagcga cgaatcgagg ccatcgaaac ggccgccggc
      841 ccgcatccgc ttcgcggcat cacaggcggc cgagaaggcc accgtggcca gccgggtcgc
      901 cgcgctgacc cgcgccacgc cgaggcggcc gagttcggcc atgtccggca ggccgggccg
      961 cacgccgacg ttcaccggcg catcgatcgc cgcgcacagc cggccgatca gctccgggct
     1021 gctcacgccg ataggataga tgcagtccgc cccggcctca agataggccc gcgcccggcg
     1081 caaggcttcc gcgacgcgcg tgtcctcatc ctcgtcgccg ccgaccatcc agttgtcgac
     1141 gcgcgcgttg atcacgatgg gcacgcccgc ctcgtccgcc gcctgccgcg ccgcggcgac
     1201 ccgccgcgcg gcgtcgtcga tatcgcgcag atactcatgg cggacgccgt cttccaggtt
     1261 gatgccggcc gcgccggtct cgatcagccg gtggacattg gccgccaccg cttcgggcga
     1321 atcgccgtag cccgcctcga aatccacagt gacgggcagc ggcgtcacgc gcacgatgcg
     1381 ctcgaccacc gccagcactt cgtccagcgg cgtgccttcg ccgtcgggat agcctagcgc
     1441 ccaggccacg cccgcgctgg tggtggcgac ggcgtcgaag ccgaggccgg caaaaatccg
     1501 cgcgctgccc gcgtcccagg cgttgggcag aagcagcacg cggcggcggt catgcagcgc
     1561 gcgaaagcgc tgcgctcgtt gaatctgtat atgcttatcc atttgtttcc cggatgaggc
     1621 cccgttgagt taccagtgat tatagcacac ggagcgaccc aggtgcagtt cgcctgtcgc
     1681 caattgcagg gggcggccgc tccatcgttc tgcaagacca gacccttcgg ccgccctttc
     1741 cccctctctc cctttccccc tttcccccct tttccctttc cccccttccc cccttcataa
     1801 ctcctaatta ttcccctgcc tgcggcgaaa gatcgccgcc gcaaccacga acagcgccgc
     1861 cgccatgacc agcagcgccg ccacctccgg cagcaccgcc gccgccccct cgccataggc
     1921 cagcttgttg aagccgtcca tcgcccagcg gttgggcgtc aggcgggcga gcgcgtcgat
     1981 gaccggcgag ctgatttgca gcgtcggaac gatgttgccg cccagcatgc cgaagatcag
     2041 cgtcaggacc atgcccagat tggcgacctg gccctcgttg gtcgcggtcg aggccagcag
     2101 cagcgcccag ccggtggcgg ccagcgccgc cgcggtgatc accagcccca cgcccggcgg
     2161 gctgccccaa ttcagccgga acagcagcga ggtcagcaat accagcaccc ccaattgcag
     2221 cacgccgctc acgaagatgg ccccgacctt gcccgacaat acctgggccg acgagaccgg
     2281 cgcggccagc atgcgggcca gtgtgccctc gcgccgctcg ctcaggaagg tgcgcgcccc
     2341 cagcgtgacg gcgtacatca ggaagaacag cgccatgccg ggggcgaaga acgacagcga
     2401 ccccaccggc gcatcggccg cgccgcccgc cgcggccgtc tcgttgtgga tggcgatcag
     2461 cgatccgccc gtcgggtcgt cgcggttgaa cagcccctgg cccatcgccc ggccgatctc
     2521 cggcagcgct tcgggcgaaa tcgcgccgct catgatcagg tcggtcatgg cgatctggac
     2581 ggtcgtcacc cctgtctgca cgcgccgggt gaactcctcg gcgatggagc gcacgatgct
     2641 cgcgccgatg gggcggccgg ggtcgccgta gacgcgcaag gcgaccgggg cgggcgttgc
     2701 gccggtcgca tcaggcagga aggagtcgct gaagccgggc gggatgatga ccgccgccgt
     2761 cgcatctccc gcgcccacgg cggcgatggc cgcggcctcg tcgtcccatt gctcggcggt
     2821 cacaaattcg ctcaggtcgt cgccggtgaa aatgtcgatc agcgtcgtgg cgaattggcc
     2881 gccgtcgtga tcgacgatga ggacggggat gttggcgatg gtcggtgcgg cgttgtcgcc
     2941 gaaaccgccg gtgacgaggc ccatggccag cgtgagggcc agcggcgcgg ccacggccag
     3001 cagcagggct gtgcggtcgc gcagcagcac gctgatgtct ttccaggtga tggcgagtat
     3061 cttgcgcata gtgaacctcc ctagtcgcgc aagccccggc cggtcagatg cagaaagacg
     3121 ctctccagat tgggttcctc gatgtcgatc gagcggatgt tgaggttggc cccgttggcg
     3181 cggccgacga cgcccgccag cgcctcgctc gcgttggtca tgttgaggac gatctggtgg
     3241 tcggcggccg cggccccgac gacgccgggc agaccggcga acagctcggc ggcggaatcg
     3301 gccgcctctt cggccaggtg cagccgcagc acttcgcctt cgcccacgac ctgggtcagc
     3361 tcggcctgcg tgccgatggc gatcagttgg ccgtggtcga tgatgcccac ccggtcggaa
     3421 agttcctcgg cctcttccat ataatgggtg gtatagagca cggtcatgcc gtcgtcgcgc
     3481 agctccttca ccatgtcgag gatgcggcgg cggctttgcg ggtcgatgcc caccgtcggc
     3541 tcgtccatga acagcagctt cggccgatgg agcagcccgg cggcgatgtt gatgcgccgc
     3601 ttcatgccgc cggagaaggt cttgatcttg tccctggccc ggtcgctcag ttccaccagc
     3661 gccagcacct cgtcgatgcg ggtggccagc gccttgccgc tcatgtcgta gaggcggccc
     3721 cagaactcca ggttctgccg cgcgctcagc gattcataga gggcgatctc ctgcggcacg
     3781 acgccgatgg cccgccgcac gcccagcgag tcgccgggga tggcgtggcc gtcgatttcg
     3841 gccgtgccgc ccgtcggctc gatcaggcag ctgagcatgt tgatggtggt ggtcttgccc
     3901 gcgccgttgg ggccgagcag gctgaatatc tcgccgcggg ccacgctgaa ggagatgccc
     3961 ttcacggctt caaggtcgcc ccacgacttg cgcaggtcgt tggcgatgag gatgggatcg
     4021 gtcatgggtc ctccgatagt cggctattcg gttgcttgct ccatatacgc gccaccggtg
     4081 tcccggtcgc gccgccacga gcatcataac cgatccaccc ccatgtctgt caagcgggaa
     4141 ccggattttt ggggcagcgc cgtagcgcaa tctgtcagat tgcctccggg caaagactca
     4201 acctggcagg ttgagctacc ccctttctcc ctttctcccc ctctcccttt ctccccttca
     4261 caactcctga tcctccatca tttcccgcag cgcctccgcc agccacgcct ccgtctccgg
     4321 ccgggccggg gcgcgcttgc ccagcagcag ggccacggtg cgcgcgccgg tgaacagcag
     4381 cgccgccagc cgccgcacct cctccaccgg cagctccccg gcgctttcgt ccagatgggc
     4441 cagcagccgc cgcagcacca gccgcaccag ctccacctcc ggcagcaagt ccggctcgtc
     4501 ggccgtcgcc gccagcacgc gctgttcggc cgccgtgggg cgggggaagt agaggtgatc
     4561 gccgggcggg ggggaagagg agaaaggggg aaggggagca ggggggaaag ggggaagagg
     4621 ggaagaggag aaagggggaa ggggagcagg ggggaaaggg gatgggggag catctcgcat
     4681 ttcgtcgttt gtcgcgcgac aggagtcgcg tcgtacctcc tccattcccg gcaaagccaa
     4741 ctgctcggcc gcctcttcat tcctgattcc tgattcgtac ttcctgattt tcttcgccat
     4801 aggagttcct ccgcgcgcat cctagcatgg tcgccgcggt tggggctatt ggataattgg
     4861 cgtgcccgta gaaac
//
LOCUS       DAOMQG010000260         4863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7302_length_4863_cov_11.129784, whole genome shotgun sequence.
ACCESSION   DAOMQG010000260 DAOMQG010000000
VERSION     DAOMQG010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7302_length_4863_cov_11.129784"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4617)
                     /locus_tag="PLC06_12525"
     CDS             complement(<1..4617)
                     /locus_tag="PLC06_12525"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRO91318.1"
                     /translation="MIAGFALLSGVRPAQADNVVIGVDRFDDDNTAEAQACSNSPNDC
                     SLRGAISLANGDIYNTYIVMLPAGTYTLDEINGEGQEDDNITGDLDIRAGMTIQGASR
                     TTVFIEPEYYINDRVIHIFQPGASGVADAGNMTVMISGVTIRNGNTPLTGGGLQIEAA
                     TLNMFDSSIVENASVGQGGGIANINSILNISDVLFDGNQSTDGDDSVSNSTECGGAIY
                     NGSTSSATIGRNTFVNNSAPWGGGGICVEGGSTTSLTMTDSTFTGNTANGYLTSGGDT
                     AGGAIYSNGNASEGTVLIENSTFDGNQSASGGAIRSNSAVFNIVNSTFTNNSAQNGGA
                     VVARTGTMTITNSTFAGNAASVEGDSWFEEVTASLNIYNSIMQGTGTGTNCFGIPDNE
                     DGNINWNGTNCPGQTVDPVLQPLADNGGSTQTMALGTGSGALEKGTVAQCPAIDQRSI
                     ARPQPVSSVTCDSGAYESAEIPPANTPPTTVLDDIPHTFILTYDFGNTIQVNLPGLFD
                     DAEDGPDLLSYHVLTITNGTAISSTLDGANILTLEGQLPLGVPASSDFTVEATDTGGL
                     SIQGTFTAQVDYPPTIDAPDTPVNAVAGTPSGAIPFIYTDDTTFGEGLSCTFSASSSN
                     TAIIPNESISFGSCNNPSISFTPTAAGSADITITAGDGRNNSRNDTFTVNVTAGEGNN
                     PPIAAGDTASVPLSGTVTMLEGGSASILTNDSDADLDTLTAQLVGGPTNAAAFTLNPD
                     GTFSYTHDGTEMVADTFTYRAYDGEAYSEPATVRIVLENNIRNDAVTIPTTTPTTDTA
                     VNSIDPNWYRFYVATPGSSISVSLQSDYDYDLYLFAPGINDEDGTIRDLAQLSDIAQL
                     SDIAQLSDIAQLSDIAQLSDIAQLSDIAQLSDIALLANIGDLIDLAQLDDIAQLSDIA
                     QLSDIAQLSDIAQLSDIAHDNVDGTLVALSIKSGPANTPGNEQVEHLVHELVGPYFLG
                     VIAHDESAVGQPFTLNITVTPEDPLPTNPTEPERDFATPATDPAVETIILTNKARIVG
                     AYSQSEADAVMTALGTLAADPTVNGVVYDLDMVQDLEGDFALHDVYAEWDDDTAAELQ
                     NPQTANRVAQTIKHFLYDAIPAYPNLKYIVLVGGDEIVPQRRMIDATLVANQRRYTQV
                     LDPAIKAAYDHRYFLSDDYYAAVLPFSAFGRELYLPQFAIGRLVESPAQIQNIIGVYL
                     NGHQTIQPQSALVTGYDFLLDQANAVAGELGARGVNNQTTLIDNSWSGADFRDQALNG
                     TRFDLISLNSHFDHKLFYPNGAATGTNLVHADELDEVTNDFTGTLVFSVGCQSGLNVS
                     DRWYAPGTPFTGADWAQMFAGEGATFIGNTGFGYGDVDLLAYSERLMLNFVQHLGYNP
                     LSNAGGNPSVGGSLMEAKQQYYNSLAAGSLSVYDEKVLSEMTLYGLPMQKIQLPNQTD
                     VEPGTAGEANAHSVTTSMAGVAEINASSNAPSIMAAVPGLDQTTLNLSFDYTLQNTSR
                     GGYYQIQGQNGLYQTAERPVLPLTTVNRDSADKVARGVLMLGGTF"
BASE COUNT          945 a         1262 c         1526 g         1130 t
ORIGIN      
        1 gaacgttccg ccgagcatca ggacgccacg ggctactttg tcggccgagt cgcggttgac
       61 ggtcgtcagc ggcaggaccg gccgttcggc cgtctgataa aggccgtttt gaccctggat
      121 ctggtaatat ccaccgcggc tcgtattctg gagcgtgtaa tcgaaactca ggttgagcgt
      181 cgtttgatca agccccggga ccgcagccat aatcgatggc gcatttgaag aggcgtttat
      241 ttccgcgact ccagccattg aggtggtaac cgaatgtgca ttggcttccc cggccgtgcc
      301 cggctcaacg tcggtctggt tgggtagctg tatcttctgc attggcaagc catagagcgt
      361 catttccgag agcactttct cgtcgtagac gctgagcgag ccggcggcca ggctattgta
      421 gtattgctgc ttggcttcca tcaatgagcc gccgaccgat gggttcccgc cggcgttgct
      481 tagcgggtta tagccgaggt gctggacgaa gttgagcatg agtcgttcgg agtaggccag
      541 cagatcgacg tcaccatagc cgaagccggt gttgccgatg aaggtcgcgc cttcgccggc
      601 gaacatctgc gcccagtccg cccctgtgaa gggcgtgccc ggcgcgtacc agcgatcgct
      661 gacgttcagg ccggactgac aacccaccga aaagaccagc gtgccggtga agtcattggt
      721 gacctcgtcc aactcgtcgg catggacaag attcgtaccc gtagccgcgc cgttcggata
      781 gaacagcttg tgatcgaagt gggagtttag cgagatcagg tcaaagcgcg tgccgttcaa
      841 tgcttgatcg cggaagtcgg cgccgctcca actattgtcg atgagcgtcg tctgattgtt
      901 gaccccccgc gcgcccaatt cgccggcaac cgcgttggcc tggtcgagca ggaaatcgta
      961 gccggtgacc agcgcgctct gtggctggat agtctgatgg ccgttcagat atacgccgat
     1021 gatgttctgg atctgagccg gagattccac gagtcggccg atagcgaatt gcggcaggta
     1081 caactcccgt ccgaaggcgc taaagggcag gacggcggcg tagtagtcat cgctcaggaa
     1141 atagcgatga tcataagctg ccttgatggc tggatccaga acctgagtgt agcggcgctg
     1201 attggcgact agcgtagcat cgatcatgcg ccgttggggg acgatctcat caccgccaac
     1261 gaggacgata tacttgagat tggggtaggc gggtattgcg tcatagagga aatgcttgat
     1321 cgtctgcgcc acgcggttgg ctgtctgcgg gttttgcaat tcggccgcgg tgtcgtcgtc
     1381 ccattcggca tatacgtcgt gcagggcaaa atcgccctca agatcctgca ccatatccag
     1441 gtcgtaaacc acgccgttga ccgttggatc ggcggccagc gtgccgagcg cggtcatgac
     1501 ggcgtcggct tcgctttggc tgtacgctcc aacgatgcgt gccttgttgg tcagaatgat
     1561 cgtctccacg gcggggtcgg tcgccggggt ggcaaaatca cgctccggct cggttgggtt
     1621 tgtcggcagc ggatcttcgg gcgttacggt tatattcagc gtgaagggtt ggcccacggc
     1681 cgattcgtca tgggcgatga cacccaggaa gtatgggccg acaagctcgt gtaccagatg
     1741 ctccacctgc tcattgccgg gtgtgttggc cgggccgctc ttgatcgaca gggcgacaag
     1801 cgtgccatcc acgttgtcgt gggcgatatc gctgagttgg gcgatatcgc tgagttgggc
     1861 gatgtcgctg agttgagcga tgtcgctgag ctgggcgatg tcgtcgagtt gggccagatc
     1921 gatcaaatcg ccgatattgg ctaacagtgc gatatcgctg agctgggcga tatcgctgag
     1981 ttgagcgata tcactcagtt gggcgatatc gctcagttgg gcgatgtcgc taagttgggc
     2041 gatatcgctg agctgggcaa tgtcgctcaa ttgagccagg tcacggatgg tgccgtcttc
     2101 gtcattgata cccggcgcga acaggtagag atcatagtcg taatcgctct gcaacgatac
     2161 ggaaatgctc gatccgggcg tggcgacgta gaagcgatac cagttggggt cgatgctgtt
     2221 gacggccgta tctgtcgtgg gtgtggtcgt tggaatggtg acggcatcat tgcggatgtt
     2281 gttttccaac acgatgcgca ccgtcgccgg ttcggagtat gcctcgccgt cgtaggcccg
     2341 ataggtaaac gtgtcggcta ccatctccgt gccgtcgtgg gtatagctga atgtgccgtc
     2401 cgggttaagc gtgaaagctg cggcattcgt cgggccgccg accaattggg ctgtcagcgt
     2461 gtcgaggtcg gcatcgctgt cgttggtcag gatggatgct gagccgccct ccagcatcgt
     2521 cactgtgccg ctcaagggaa ccgaagctgt gtcgcctgct gcgattggtg gattgttgcc
     2581 ttcgcccgcg gtgacgttga ccgtgaacgt gtcattgcgc gagttgttgc ggccgtcgcc
     2641 ggcagtgata gtaatatccg cgctgcccgc tgccgtggga gtaaatgaga tcgatgggtt
     2701 gttgcacgag ccaaacgaga tggattcgtt ggggatgatc gccgtgttgg atgaggatgc
     2761 cgagaaggtg catgaaagcc cttcgccgaa ggtcgtatca tcggtgtaga tgaacgggat
     2821 tgctccggaa ggcgttccgg caaccgcgtt cactggtgta tcaggcgcgt cgatagtggg
     2881 cggatagtcc acctgcgccg tgaatgtgcc ctggatgctg aggccgccgg tatccgtggc
     2941 ctcaacggtg aagtcggatg aggcaggtac tcccagcggc agttggcctt cgagggtcag
     3001 gatgtttgcc ccgtcgaggg tcgaagagat ggctgtcccg ttggtgatag tcagcacgtg
     3061 ataggacaga aggtcaggtc catcttcggc gtcgtcaaac agtccgggaa gattgacctg
     3121 gatcgtgttg ccgaaatcat aggtgaggat gaatgtatgc ggaatatcgt ccagtacggt
     3181 tgtcggcggg gtgttggccg ggggtatctc ggcgctctca tacgcgccac tgtcgcaggt
     3241 aacagaagac acaggttgcg gccgcgcgat gctgcgctgg tcgattgccg ggcattgtgc
     3301 gacggtgcct ttctccagtg ccccgctgcc cgtgcctaaa gccatcgttt gtgtagatcc
     3361 accgttgtcg gccaatggtt gcaggacggg atcgactgtc tgccctgggc agttggtgcc
     3421 gttccagttg atgttgccat cctcattgtc agggatcccg aaacagtttg tgccggtgcc
     3481 ggtgccctgc atgatgctgt tgtagatgtt cagagaggcg gttacttcct cgaaccagga
     3541 atcaccttca accgaggctg cattgcccgc aaaggtggag ttggtgatgg tcatcgtacc
     3601 ggtgcgcgcc acaaccgcgc cgccgttttg agcgctgttg ttggtgaagg tgctgttgac
     3661 gatattgaac acggccgaat tgctgcggat agccccaccc gaggcgcttt ggttgccgtc
     3721 gaacgtacta ttctcaatga gaactgttcc ctccgacgca tttccatttg aatagatagc
     3781 ccctccggcc gtatcgccac cggaagtcag atagccgttg gctgtattgc cggtaaacgt
     3841 gctatcagtc atggtcaacg aggtggtcga accgccctcg acgcagatgc cgccgccgcc
     3901 ccagggggcg ctgttgttga caaaggtatt cctgccgatg gtagcactgc tggttgatcc
     3961 gttataaatg gctccgccac attcagtcga attgctgaca gagtcgtcgc cgtcggtcga
     4021 ttgattgccg tcaaagagaa cgtcggagat gttcaggata ctgttgatgt tggcgatgcc
     4081 gccaccctgc ccgaccgagg cattctcaac aatagaactg tcaaacatgt tgagggttgc
     4141 cgcttcaatc tggagtccgc cgccggtcag gggagtgttg ccgttccgaa ttgtgacgcc
     4201 actgatcatg acggtcatat tgcccgcatc ggccacaccg cttgcgccgg gttggaagat
     4261 atggatgact cgatcgttga tgtaatattc gggttcaata aaaaccgtgg ttctgcttgc
     4321 cccctgaatt gtcatgccgg ctctgatatc caggtcgccg gtgatgttat catcctcctg
     4381 accttcgccg ttgatctcgt caagggtgta ggttccagcc ggcagcatga cgatataggt
     4441 attgtatata tcgccattgg ccaggctgat ggcgccgcgc aaggagcagt cgttagggga
     4501 attcgagcag gcttgagcct cggcggtgtt gtcgtcgtcg aaccgatcga cgccaatgac
     4561 cacattatcg gcctgcgccg ggcgcacgcc ggagagaaga gcaaaacctg caatcattcc
     4621 cagaaggaaa atcggttgcc atttaaattt gtcggcgatg tgagagcgat gtaggcgaaa
     4681 catgagaatc ctccactaca ttacagcttg catgcgaatc gactaatcag aatttgtcac
     4741 ctgattgata gagcaacatt ccgcaatacg tactatacac taaaatcaga gcgagttctg
     4801 tcatctaggc gtcataactc tatgaatttt tattaaaatg tccttaaata aggcctctaa
     4861 tag
//
LOCUS       DAOMQG010000261         4862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7305_length_4862_cov_9.883919, whole genome shotgun sequence.
ACCESSION   DAOMQG010000261 DAOMQG010000000
VERSION     DAOMQG010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7305_length_4862_cov_9.883919"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLC06_12530"
     CDS             complement(<1..319)
                     /locus_tag="PLC06_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91319.1"
                     /translation="MSLTQQSSANASSPSRLVIPFVDTQPLTDIKLAILMLPVWWALG
                     VEQFIWPILFGIASIKTVHLQDLRLVVTRPLRWFGLFLIAVLISGLFIEESMRYATFA
                     RNLG"
     gene            complement(379..1503)
                     /locus_tag="PLC06_12535"
     CDS             complement(379..1503)
                     /locus_tag="PLC06_12535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO91320.1"
                     /translation="MVHWHFTWQNQQNLARGLAERGYQVYFIEPLPKRWPRINEFGRV
                     WGRLTGQSEAAGICRQPLIPGVELVSPRMLPDTGTLTRWVNRRLFLPGLEDKLKESIQ
                     RPLIVINDLPIPASLELMNSLEPDASFYDCVNDWANDPYMPIHDFERSLAGAVDMVWA
                     DSRANVVRVSSMNKTVVQLPPDVDIALFSRARNDPGPLPSRPLCAYFGSISLSTDIDL
                     LRMVSHQYPLRLIGPLRTTLNGFSDKTEVVGPVPHHQLPELLRDVDVLLLPYVHSAHN
                     ASVMPAKLYECLATGKPTIVSGLTTLHDYSDLFYIRETPEAFIEAIAEVVHEPEAYRA
                     KRIAAAEAHSFDHRMDVVEGYIQQVLEQKSRHSREYKPAR"
     gene            complement(1582..2502)
                     /locus_tag="PLC06_12540"
     CDS             complement(1582..2502)
                     /locus_tag="PLC06_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRO91321.1"
                     /translation="MINILYIAGAGRSGSTLLERILGQLDGVVNVGELRHAWRHAPTA
                     QRCGCGEILSECTYWQEVMARTQFPLTAEGFADLYAAQRKVDRIRYIPGMLNKSLGSD
                     EFIRNHAIYTGTLRKIYTAIHDLTGSHTIVDASKDMSTLYLLRETADLRLRVLHLVRD
                     SRAVAYSWTREKVRADLVDETAYIGRYSPQHSALDWIYRNQMTEMARGRVDGYIRLRY
                     EELVANPLEVIERIARFIELPESDLGFINDGEIQFSKESHALAGNPMRFQEGGVKLKM
                     DSAWQQEFGKADKAVVTALTWPMLRRYNYL"
     gene            complement(2499..4046)
                     /locus_tag="PLC06_12545"
     CDS             complement(2499..4046)
                     /locus_tag="PLC06_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRO91322.1"
                     /translation="MGLDANMGQAPVPDEVNEIADTSINRMATGAGISLFGVGLGRGL
                     DFVKQIALARLLGPEAFGLYAIGWNLLRIVGILAPFGLHNGVVHFGTPFWRKDNPALR
                     SILTRSVLLSFAIGWVITGVLLLLAPWLTASIFKEPEFLPLFRVFALMLPFMGALLVA
                     ANATRITQRMIFSVASEEIGQGALNLILFIVLYLLGWHLLGAIVATVASFALAFGLSL
                     YFLWRLFAPAFAGPSRPAISNRTLLAYSLPTALAGMFGVIIGRVDRLFLGYYWPSEEV
                     GVYQAAAQLSVIMALVLNAFNMILMPMIAEQYHKRDMKQLEELFRVNTKWGIYCIIPV
                     VLVILFAAGDVMTVLFGSEYTRGSTALFILTIGQFINIATGATGTILIMTGRQTAWFR
                     LSMLILAINLALNLTLIPIWGMNGAAVATSVTVGGLFALGLVVVHRIHHIWPYDRRYL
                     KGVLAAAVTAALLVGVRLLQLESTLNLLVTTLVAAVGFYGLLLLLGLDTEDRTFVESI
                     LRRVRAK"
     gene            complement(4036..>4862)
                     /locus_tag="PLC06_12550"
     CDS             complement(4036..>4862)
                     /locus_tag="PLC06_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO91323.1"
                     /translation="HELTGVLTLSPEEQIAWRGIALVRMTQGKLNEVAAAYLHVNNYR
                     AELLDWGARAETQRQWAKAQDWYRVAILLEPENGDHYYRLARISAEQGESEAKAYYVQ
                     ALSAPRHTEFGRSNILTRLGELEKRKSSTDWNEVLARFDEAIQLNEFVDQQDIIQARS
                     NKAEALDRLGKYRAALDEYESLAGDYPGNYWANVHSGRLVWQLKSDAPTAIAYLEKAI
                     QNNDKPKWAYLLLAQIHAQSGNPELAIPLFRKVLTIDPGDRAAREQLDKLTGGDGS"
BASE COUNT         1122 a         1325 c         1387 g         1028 t
ORIGIN      
        1 caccgaggtt tcgcgcgaat gtggcgtagc gcatcgactc ctcgatgaag aggccagaga
       61 tcaggacggc gatgaggaat agcccaaacc agcgcaaggg ccgagtgaca accagccgca
      121 agtcctggag atgaacagtt tttatggaag cgatgccgaa gaggatcggc cagataaatt
      181 gctcgacgcc caacgcccac caaaccggca acatcagaat cgccagcttg atatccgtca
      241 atggctgcgt atctacgaac gggatgacca aacgagaggg ggatgaagcg ttggccgatg
      301 attgttgcgt cagggacata ttgatccgac ttgcgaccag agacggccgc gccttggcta
      361 gcgatgactg ctcaacattt accgggcagg cttgtactcc ctggagtgtc ggcttttttg
      421 ttccagcacc tgctggatat atccttcgac cacatccatc cgatgatcaa aactatgggc
      481 ttccgcagcc gcaatacgtt tggcacgata cgcttcaggt tcatgaacca cttctgcgat
      541 tgcttcaatg aaagcttcag gggtttcacg tatataaaag aggtcagagt aatcgtgtaa
      601 tgttgtgagt cccgagacga tggtgggttt gccggtggcc agacattcgt aaagcttggc
      661 cggcatgacg ctggcgttat gggctgaatg gacatagggt agcaacaaca catccacatc
      721 acgtaataat tcgggtagtt gatgatgtgg cacggggccg accacttcag ttttgtctga
      781 gaaaccatta agggtggtgc gcagtggccc aatcaaccgc agcggatatt ggtggcttac
      841 catccgtaac aaatcgatat cagtgctgag gctgatggag ccgaaatagg cacataatgg
      901 ccggcttggt agcggtcctg gatcatttcg tgccctggaa aatagagcaa tatcaacatc
      961 tggaggtagt tgcacgacgg ttttattcat gctcgacaca cggacaacat tcgcccgaga
     1021 gtcagcccac accatatcaa cggctccagc caaactgcgc tcgaagtcat gaattggcat
     1081 gtagggatca ttggcccagt cattgacgca gtcgtagaag ctggcatcag gttccagact
     1141 gttcatcaat tccagcgaag ccggtatggg cagatcgttg atcacaatga gcggtcgttg
     1201 aattgattcc ttcagtttgt cttcaaggcc tggcaaaaaa agacgccggt taacccatcg
     1261 cgtcaatgtc ccggtgtcgg gcaacatacg tggtgagaca agctcgacgc cgggaatcag
     1321 aggctgccgg caaatcccgg cggcttcgct ttgccctgtc agacgtcccc aaacccggcc
     1381 aaattcattg attcgtggcc agcgcttggg caacggctca atgaagtaaa cctggtagcc
     1441 acgttcggcc aaccctctgg ccaggttttg ctgattctgc cacgtgaaat gccagtggac
     1501 cattgatatg agtatgacgg ttcccttcat aaagctcaac ccaagcggcc ggatatcaaa
     1561 tcggcttggc tattgtatcc cttacaggta attatagcgg cgcaacatgg gccaggttaa
     1621 tgcagttacg actgccttgt cggctttccc gaactcctgt tgccaggcgc tatccatttt
     1681 caacttcacg cctccttcct ggaaacgcat cggatttccg gctaaggcat gactttcttt
     1741 ggaaaattgt atctctccgt cattaatgaa gccaaggtcg gactccggta gctcgataaa
     1801 acgggcgatg cgttcgatga cttctagtgg attggcgacc aattcttcat aacgcaggcg
     1861 gatgtatccg tcgacccggc cacgggccat ttccgtcatc tgattgcgat agatccagtc
     1921 gagggcggaa tgttgcggtg aataccgccc tatgtaagca gtttcatcaa cgagatcggc
     1981 ccgcactttt tcccgtgtcc acgagtaggc cacagctcga ctgtcgcgca ccaggtgcag
     2041 cacgcgcagg cgcaaatctg ccgtctcgcg caggagataa agtgtagaca tatcttttga
     2101 ggcgtcgacg atagtatggc tgccggtcag atcgtgtatg gcggtataga tttttcgcaa
     2161 tgtgccggta tagatggcat ggttgcggat aaactcgtcg ctgccaagtg atttgttgag
     2221 catgcctgga atataacgaa ttcggtccac cttgcgctgg gctgcgtaca gatcggcaaa
     2281 gccctcagcg gtcaatggaa attgggttcg cgccatcacc tcttgccaat aggtgcattc
     2341 gctgagtatt tcaccgcacc cacatcgttg agcggttgga gcgtgacgcc atgcgtgccg
     2401 caattcgccg acgttgacga cgccatccag ttggccgagg attcgctcca gcagggtgct
     2461 gccgctgcgg ccggcgccgg cgatatagag aatgttgatc attttgccct gacccgtcga
     2521 aggatcgatt cgacgaatgt gcggtcttct gtgtcgaggc ccagcagcag aagcagacca
     2581 taaaagccta cggccgccac cagcgttgtc accaacaaat tgagcgtcga ttccagctgg
     2641 agcaggcgta ctccgaccag cagggcggcc gtcaccgcgg cagccagcac acctttcagg
     2701 taacgtcgat cgtagggcca gatatggtgg atgcgatgga cgaccaccag ccccagcgcg
     2761 aacagcccgc caacggtgac cgatgtcgcc actgccgcgc cattcattcc ccagattgga
     2821 atcaaagtca agttgagcgc cagattgatc gcgaggatta acatgctcag ccggaaccag
     2881 gctgtctgcc ggccggtcat gatgagaatc gtccccgtcg cgccggtggc gatattgatg
     2941 aactgtccga tggtgaggat aaacagcgcc gtcgatccgc gcgtgtactc gctgccgaac
     3001 aggacggtca tcacatcgcc ggcggcaaag aggatcacca gtacgaccgg gatgatgcag
     3061 taaatacccc atttggtatt cacccggaac agttcctcca attgcttcat gtcgcgcttg
     3121 tgatattgct cggcgatcat cggcatgagg atcatgttaa atgcattgag caccagggcc
     3181 atgatgacgg agagctgtgc cgccgcctga tagacgccga cctcctcaga cggccaataa
     3241 tagcccagga acaggcggtc gacgcggccg atgatgactc caaacatgcc cgccagcgcc
     3301 gtaggcagcg aataagccag caacgttcga ttggagatgg ctggccgcga tgggccagca
     3361 aatgccggcg caaaaagccg ccaaagaaaa tagagcgaca gaccaaaggc caaggcaaat
     3421 gatgccacgg tcgccacgat tgcgcccaga agatgccagc ccagcagata gagcacgatg
     3481 aagagaatca gattgagcgc tccctggcca atctcttccg aagccacgga gaagatcatg
     3541 cgctgggtga tacgcgtggc gttggcggcg acaagcaacg cgcccataaa cggcagcatg
     3601 agggcgaaga cgcgaaacag cggcagaaac tccggctcct tgaatatgct cgccgtcagc
     3661 cagggagcca gaaggagaag aacgcctgtg atgacccagc cgatagcgaa gctcagcaga
     3721 accgaacggg tgaggatgct ccgcagtgcc gggttgtcct tgcgccaaaa gggcgtgccg
     3781 aaatggacga cgccgttatg aaggccgaag ggggccagga taccgacgat gcgcagaaga
     3841 ttccagccaa tggcgtatag cccgaaggcc tccgggccaa gcaagcgggc cagcgcgatc
     3901 tgcttgacga aatcaaggcc gcgacccagc ccgacaccga acaacgagat tccggcgccg
     3961 gttgccatgc ggttgatgct ggtgtctgcg atctcgttta cctcatcggg aacgggggcc
     4021 tggcccatgt ttgcatcaag acccatcgcc acccgttagc ttgtcgagtt gttcccgtgc
     4081 cgcccgatcg cccggatcga tggtcaatac tttccggaat agcgggatgg ccaattcggg
     4141 attgcctgat tgggcgtgaa tctgggcgag aagcagataa gcccatttgg gtttatcatt
     4201 gttttgaatc gccttttcca gataggcgat tgctgttggc gcgtctgact tcaattgcca
     4261 gaccaggcgg ccgctatgga cattggccca atagtttccg gggtaatcgc ccgccaaaga
     4321 ctcatactca tcgagcgcgg cgcgatattt gccaaggcgg tccaacgctt cagccttgtt
     4381 tgaccgggcc tggatgatgt cctgttggtc gacaaattcg ttcagttgga tggcctcgtc
     4441 gaaacgagcc agtacctcgt tccagtcggt cgacgatttg cgtttctcaa gctcgccgag
     4501 acgtgtcaga atgttgctgc ggccgaactc ggtgtggcga ggggcactta aagcctggac
     4561 ataatatgct ttagcttcag actcgccttg ctcggccgat atgcgggcca ggcggtagta
     4621 gtgatcgccg ttctccggtt ccaggaggat ggcgacgcga taccaatcct gggctttggc
     4681 ccactgcctt tgggtttcgg ctctggctcc ccaatccagc aactccgccc ggtagttatt
     4741 gacgtggaga taggctgcgg cgacctcatt cagcttcccc tgtgtcatgc gcaccagggc
     4801 aatgccacgc caggcgatct gttcctcggg tgaaagagtt aaaactcccg tcaactcgtg
     4861 ag
//
LOCUS       DAOMQG010000262         4861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7308_length_4861_cov_9.065335, whole genome shotgun sequence.
ACCESSION   DAOMQG010000262 DAOMQG010000000
VERSION     DAOMQG010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_4861_cov_9.065335"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /gene="tkt"
                     /locus_tag="PLC06_12555"
     CDS             complement(<1..1956)
                     /gene="tkt"
                     /locus_tag="PLC06_12555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRO91324.1"
                     /translation="MQESIELQNQAINTIRFLSADAIEKANSGHPGLPMGTAAMAYAL
                     WTRHLHHNPTNPRWANRDRFILSGGHGSMLLYSLLHLTGYDLGLDELKNFRQWNSRTP
                     GHPEYGLTPGVDVTTGPLGQGFANGVGMAIAEAHLAAVFNRPGHDIIDHYTYAIVTDG
                     DLMEGVASEAASLAGHMQLGKLIYLYDDNHISIEGSTDIAFTEDRGKRFEAYGWQVLY
                     VADGQDVDAIDRAISEAQSDARPSLIIVRTIIGYGLPTRAGTAKAHGEPPGEAELNGA
                     KENLGWPLEPRFYIPDDVLSFYRRAIEEGTTREAEWQVRFDAYRAAHPDLAAELTRRL
                     AGELPVDWDKGLPEFPADAKGIATRSSSGKVINALAARMPELFGGSADLAPSTKTWID
                     HDPSFDLNHAGRNVHFGVREHAMGAIVNGLTYHGGTIPYGATFLVFSDYMRPAIRLAA
                     LSHLGSITVFTHDSIGVGEDGPTHQPIEQLAALRAIPNLLVLRPADANETREAWIMAI
                     TDRDRPSALVLTRQSLPTLDRTVMASADGLRRGAYVLADLGGGRPEIILMASGSEVSL
                     IVEAGQRLAEQGHSVRLVSFPSWELFDEQDEAYRAEVLPADITRRVAVEAGVAQGWHR
                     WVGDAGRVIALDRFGASAPAKVVFEQLG"
     gene            complement(1999..3414)
                     /gene="gndA"
                     /locus_tag="PLC06_12560"
     CDS             complement(1999..3414)
                     /gene="gndA"
                     /locus_tag="PLC06_12560"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006197555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HRO91325.1"
                     /translation="MKKQYALGIMGLGVMGRSLALNFERHGYPVVGYDPKPHLPADFP
                     STVVDSPAALVAALDAPRVVLIMVPAGKPVDTAIASLKDSLQAGDIIIDGGNSYFLDT
                     ERRDKELAGVGLHFVGMGVSGGESGALWGPSLMPGGEQTAWETIAPMLRAIAAVAEDG
                     EPTVTWIGPGGAGHYVKMVHNGIEYGDMELIAEIYDLLHRGAGLSNAELADLFDGWNK
                     GELRSYLIEITAKVLRYVDEETGRPLVDLIMDIAGQKGTGRWTSQIALEVGAPTPTIN
                     AAVVMRLLSADKETRLPVAARLGGTTAFTGDRDRLVAAAEAALYAGKVTAYAQGMDLI
                     RTASKDYDWNLNIAAIVRIWRAGCIIRAQLLEGIAAAFERHPDLPNLLLDDTVATAIK
                     QRQADWRAVVIAGVEMGIPLLALAAALGYFDALRSERLPANLIQAQRDFFGAHTYHRI
                     DRDGVFHTEWEQPLPKPGLSK"
     gene            complement(3427..3876)
                     /locus_tag="PLC06_12565"
                     /pseudo
     CDS             complement(3427..3876)
                     /locus_tag="PLC06_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
     gene            complement(3970..>4861)
                     /locus_tag="PLC06_12570"
     CDS             complement(3970..>4861)
                     /locus_tag="PLC06_12570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO91326.1"
                     /translation="PQALPGHYGIGAVDAGDYDTPRDVEYVIGAATALRRSLLDEIGL
                     LDEGYFLYFEDADLCARARRAGHRVAYWPAATGIHVESATAAKGSFAYLHRFHSGRWR
                     YLLKHFPAAEIIHETLPAEVAWLERLAPTERRAVSLAWLSTRSQLPQILAARAREGEG
                     ALSTEETAVIGEGLAVLRQKALAASFDPASLARLSAAAELHERPFTSDVPLLGPLIAR
                     FRTAWNNVASRWYLGHLMSQQNEFNRLAVAEMSRLEQELREQMALLEEQIVAGVELRE
                     EIDRLQARLAELAALMPLSS"
BASE COUNT          841 a         1539 c         1605 g          876 t
ORIGIN      
        1 ccctaactgt tcaaaaacta ccttggccgg ggccgatgcg ccgaaacgat ccagcgcaat
       61 gacgcgcccc gcgtccccca cccaacgatg ccagccctga gccacgcccg cctcgacggc
      121 cacgcggcgg gtgatgtccg ccggcagcac ctcggcccga taggcttcgt cctgctcgtc
      181 gaaaagctcc caactgggga acgataccag ccgcacgctg tggccctgtt cggccagccg
      241 ctgcccggcc tcaacgatga ggctcacctc ggaaccggac gccatcagga tgatctccgg
      301 ccgcccgccg cccaggtcgg ccagaacgta agcgccgcgg cgcaacccgt cggccgaagc
      361 catcacggtg cggtcgagcg tgggcagcga ttggcgcgtc aacaccagcg ccgaaggccg
      421 gtcgcggtcg gtgatggcca tgatccacgc ctcgcgcgtc tcgttggcgt cggccgggcg
      481 caggacgagc aggttgggga tggcccgcag cgcggccagt tgttcgatcg gctggtgggt
      541 cggcccgtct tcgcccacac cgatgctgtc gtgagtgaag acggtgatcg atcccagatg
      601 cgacagggcc gccagccgga tggccgggcg catatagtcg gaaaagacga ggaaggtagc
      661 cccatagggg atagtgccgc cgtgataggt caggccgttg acgatagccc ccatcgcgtg
      721 ctcgcggacg ccgaaatgga cgttgcgccc ggcatggttc aggtcgaacg atgggtcatg
      781 gtcgatccac gtcttggtcg agggcgccag gtcggccgag ccgccgaaca gttccggcat
      841 cctggcagcc agcgcgttga tcaccttgcc cgatgagctg cgcgtggcga tgcccttggc
      901 gtcggccggg aattcgggca accccttgtc ccaatcgacc ggcagctcgc ccgccagacg
      961 gcgcgtcaac tccgcggcca ggtcgggatg ggcggcgcga taggcgtcga aacggacctg
     1021 ccattcggcc tcgcgggtcg taccttcctc gatcgcccgg cggtaaaagc tcagcacgtc
     1081 gtcggggata tagaagcgtg gctccagcgg ccaaccgaga ttttccttgg ccccgttcaa
     1141 ttcggcttcg cccggcggct caccgtgggc cttggctgtg cccgcgcggg tcggcaggcc
     1201 atagccgatg atggtgcgca cgataatgag cgacggccgg gcgtcgcttt gggcctcgct
     1261 gatggctcga tcgatggcgt ccacgtcctg gccgtcggcc acgtagagca cctgccagcc
     1321 gtaagcctcg aagcgcttcc cgcggtcttc ggtgaaggcg atatcggtgg agccttcaat
     1381 ggagatgtgg ttgtcgtcgt agaggtagat cagcttgccc agctgcatgt ggccggccag
     1441 cgaggcggcc tcggatgcca cgccttccat caggtcgccg tcggtgacga tggcgtaggt
     1501 gtagtggtcg atgatgtcgt ggccggggcg attgaagacg gccgccagat gagcttcggc
     1561 gatagccatg ccgacgccgt tggcgaatcc ctggcctaat gggccggtgg tcacatccac
     1621 gccgggcgtc agaccatatt cgggatggcc gggcgtgcgg ctgttccatt ggcggaagtt
     1681 cttcagctcg tccagcccca aatcgtagcc ggtcaggtgg agcaggctgt agaggagcat
     1741 ggagccgtgg ccgccggaga ggatgaaacg gtcgcggttg gcccagcggg ggttggtcgg
     1801 gttatggtgc agatggcgcg tccagagggc gtaagccatg gcggccgtgc ccatcggcag
     1861 gcccggatga ccggagttcg ccttctctat ggcgtcggcc gacaggaagc ggatggtgtt
     1921 gattgcctga ttttgtagtt caattgattc ttgcatgatc aaagttctct tttggatttt
     1981 tccagcccgg aaaccggttc atttcgataa gcccggtttc ggcaaaggct gttcccactc
     2041 ggtatggaat actccgtccc gatcgattcg atgataagta tgcgccccga aaaagtcgcg
     2101 ctgagcctga atcaggttgg ccggcagccg ttccgagcgc agcgcgtcga aatagcccaa
     2161 tgcggccgcc agcgccagga gcgggatgcc catctcgacc ccggcgatta ccaccgcgcg
     2221 ccagtctgcc tgtcgctgtt tgatggctgt ggcgacagtg tcatccagca gcaggttggg
     2281 caggtccgga tgtcgctcga aagcggcggc aataccttcc agcaactgcg cccgaatgat
     2341 acaccccgcg cgccagatgc gcacgatggc ggcgatgttg agattccagt cgtaatcctt
     2401 gctcgccgtc cggatcaggt ccatgccttg cgcgtaggcg gtcacctttc cggcatagag
     2461 cgcggcttcg gccgcggcca ccagtcgatc gcggtcgccg gtgaaagctg tcgtgccgcc
     2521 cagccgcgcc gcgaccggca ggcgtgtctc cttgtcggcg ctcagcagtc gcatgacgac
     2581 ggcggcgttg atcgtcggtg tcggcgcgcc gacctcaagg gcgatctggc tggtccaacg
     2641 gccggtgccc ttttgtccgg cgatgtccat gatcagatcc accagcggcc ggccggtctc
     2701 ctcgtcgaca tagcgcaaca ccttggccgt gatctcaatc agataggagc gcaattcgcc
     2761 cttgttccaa ccgtcgaaca ggtcggccaa ttcggcattg gacaggcccg cgccgcggtg
     2821 gagcaggtca tagatctcgg cgatgagttc catgtcgccg tactcgatgc cgttgtggac
     2881 cattttgaca taatggcccg cgccgcccgg cccaatccag gtcacggtcg gctcgccgtc
     2941 ttcggccacg gcggcaatgg cgcgcagcat gggggcgatg gtttcccagg ccgtctgttc
     3001 cccgccgggc atcaggctcg gcccccacag cgcgccgctc tcgccgccgg agacgcccat
     3061 gccaacgaag tgcaggccca cgccggccag ttccttgtcc cgtcgctctg tatcgagaaa
     3121 ataagagttg ccgccatcga tgataatatc accggcttgc aatgaatctt tcagtgaggc
     3181 gatggcggta tccaccggtt tcccggcggg caccatgatc agcacgacgc gcggtgcatc
     3241 gagtgcggcc accagcgccg ccggcgaatc gacgaccgtt gagggaaaat cggccggaag
     3301 atggggtttg ggatcataac cgacgaccgg gtagccgtga cgctcgaagt tgagggccag
     3361 gctccggccc atgacgccca atcccatgat acccaacgcg tactgcttct tcataagctt
     3421 ctccataatt gaattgataa ttgcatccgg ctcggcggcg acgctgacct gaatggcatg
     3481 ggccggctcc tccagatcgt cgaactgact gcgcagcatc tccgcccgca tgtagtgccc
     3541 ttcgcgtcgc tgcagtcgcg tccaaatcgt gtcgtagtcg cctttcaggt agacgaaccg
     3601 cacccgtcgg ggatcgacgc gcaagacatc acgatacttc tttttcaggg cggagcaagc
     3661 gatgacgccg ccctgatcgc gggccaggtc gtcgcgaatg agactcgcca gagccgccag
     3721 ccaaaccgcg cgatcttcat ttcttagagg aaccccggcg gtcattttgg caacattggc
     3781 cgaggggtga aagtcgtcgc cgtcgtggaa cggtacgccc aggcgcacgg ccagttcgcg
     3841 gccgatagtc gttttgccgc tgccggccac gcccatgacg atgatgcaac atggatcgtg
     3901 ccccatcggg atcagattcc gccacatggc atgcggttat tcatataaca atgaactgat
     3961 attagtcagt tatgatgaga gcggcatgag agcggcaagt tcggcgagcc tggcctgcag
     4021 ccggtcgatc tcctcgcgca gttcgacgcc ggccacgatc tgctcttcca gcagcgccat
     4081 ctgttcccgc agttcctgct ccagtcgtga catctcggcg acggccagcc ggttgaactc
     4141 gttctgttgg ctcatcaggt ggccgagata ccagcgggag gcgacgttat tccaggctgt
     4201 ccggaagcgg gcgatgagcg ggccgaggag gggcacgtcc gaggtaaacg gccgctcgtg
     4261 cagctcggcc gcggctgaca agcgggccag gcttgccggg tcaaacgacg cagccagagc
     4321 tttttggcgc aagaccgcca gcccctcgcc gatgacggcc gtttcttcgg tcgatagcgc
     4381 cccctctccc tcgcgggcgc gagcggcaag gatttggggc agctggctcc gggtggagag
     4441 ccaggccagc gagacggcgc gtcgctcggt tggggccagc cgctcgagcc aggccacctc
     4501 ggccgggagg gtctcatgga tgatctccgc cgccgggaaa tgtttcagca gatagcgcca
     4561 acggccgctg tggaagcggt gaagataggc gaagctaccc ttggcggcgg tagccgactc
     4621 gacgtggatg ccggtcgccg cgggccaata ggcgacgcga tggcctgccc gccgggcgcg
     4681 ggcgcagaga tcggcgtcct cgaagtagag gaagtagccc tcatccagca gcccgatctc
     4741 gtcgagcagg ctgcggcgaa gggcggtggc ggcaccgatg acgtactcca cgtcgcgggg
     4801 ggtgtcataa tcgcccgcat ccaccgcgcc gatgccgtag tggccgggca gcgcctgggg
     4861 g
//
LOCUS       DAOMQG010000263         4861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7309_length_4861_cov_6.450895, whole genome shotgun sequence.
ACCESSION   DAOMQG010000263 DAOMQG010000000
VERSION     DAOMQG010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7309_length_4861_cov_6.450895"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1326)
                     /locus_tag="PLC06_12575"
     CDS             complement(139..1326)
                     /locus_tag="PLC06_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HRO91327.1"
                     /translation="MDTTTTPTKPHAPRWVLALLPLLILAALIAGFLALDPLSFFRGA
                     FPPIETLAVQRITFPAPGSIRLEVINGGPERTTISQLTIDDAYWQFAISPDNVLDRLE
                     TATIDIPYPWVNGEPLAINLITSTGLTFPATAAVAVKSPAVDGRTLLAYALLGIYVAI
                     IPVGLGMLWYPFMRRIDRKWMNAILALTVGLLVFLFVDMVLEALDQAHAAPAVFQGVP
                     VVIFGILLSLGVLLAVGRRSGEKSPQAVSTLIALGIGLHNLGEGLAIGAAFATGAAAL
                     GTFLVIGFTLHNITEGIGIVAPLTRGERPRLRTFLLLALLAGGPAVLGTWIGGLAFSP
                     FLAALFLSIGAGAILQVIWEVSKMLVRDAQRAGGSAMTWLNFGGLAAGIVIMYLTAFL
                     VKF"
     gene            complement(1426..2406)
                     /locus_tag="PLC06_12580"
     CDS             complement(1426..2406)
                     /locus_tag="PLC06_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009815501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRO91328.1"
                     /translation="MGMIGQVDHAANGFDPHEILTDFDYGTVTQENGRTVRTWQIYAL
                     DKEIEIAPGVMFPGWLYGSPTSAASRRAGKLVPQCPGPTLRCVEGERLRIEFGNGGSH
                     PHTIHFHGIHSAAMDGVPGVGAGQIQPGGRTVYEFDAKPFGCHLYHCHAVPLKRHIHK
                     GLYGAFIIDPDPEKYSGEEAALARARNHTYPECQAIHEMVMVLNGFDTNFDSANEIYA
                     ANTVAFGYGMERPIAIRREQRQRLYLINILEFDPINSMHLHANFFDYYDHGTTLRPTQ
                     VTIDTVMQCQAQRGILEFSFAEHEPGLYMFHAHQTELAELGWMGFFEVKG"
     assembly_gap    2410..2509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2678..3877
                     /locus_tag="PLC06_12585"
     CDS             2678..3877
                     /locus_tag="PLC06_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRO91329.1"
                     /translation="MSDQPVTILCLSGYFKGLNFLITAKALGARVLLLTRENNAEEDW
                     PYDSIDRFMIMPDIRKRPDIFHAVSYLARSESIDVIVPLDDYDVETAAALREHLRLPG
                     LGETQARYFRDKLAMRLQAAAAGIPVPPFTPTFTYDRLREYMERVPPPWVLKPRSEAG
                     AMGIKKMYSAEEVWRRLDELGDEQSNFHLEQFVPGEIYHVDSLVYDGELLFAQPHKYA
                     RPPLTVSHDGGVFASRTLPDGEEARAILGLNARLLRAFGLQRGAAHAEFIRGADGQFY
                     FLEVAARVGGANIENLIEAATGLNPWGEWARMEIAYAQGQPYTVSPTRHVYAGLLVCL
                     ARQEWPDLSGYNDPEVVYRVHKKQHAGLVVASPDPGRVEWLLNQYAERFGRDFLAVLP
                     ALDKAPL"
     gene            3992..4624
                     /locus_tag="PLC06_12590"
     CDS             3992..4624
                     /locus_tag="PLC06_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91330.1"
                     /translation="MGLELLCAVVIALGFGVLVAFSGYRLFLSLLPIWGFFFGFVLGT
                     QTIQALIGDGMFATATSWVVGFVVALVFAVLSYLFYFWAVAVVAGSLGYGVGVGLMNL
                     IGLDFALIPWIVGIILGAVLIVVTFRYNLAKVVIIAATAVGGAALAIGTLALGPGNVS
                     LGSAFNNPVQAVLGLGFLWAILFVIMAAAGFYVQWQANRAWTAEPYPNRI"
BASE COUNT          834 a         1494 c         1505 g          928 t
ORIGIN      
        1 gaattacgaa ttacgaatta cgaattacga attacgaatg atgaattacg agtacgggcg
       61 cgcaaacctg acaggtgggc ggcgacaaaa ttcgctctca tcagagtttg tcttgccgcc
      121 cacctgtcag gttttgactt agaacttcac caaaaacgcc gtcagataca taatcacgat
      181 ccccgccgcc agcccgccga agttgagcca ggtcatggcc gagccgccgg cgcgctgcgc
      241 gtcgcgcacc agcattttgc tgacttccca aataacctgc aggatggccc ccgcgccgat
      301 gctgaggaac agcgcggcca gaaatggcga aaacgccagc ccgccgatcc acgtgcccag
      361 caccgccggg ccgcccgcca gcagcgccag cagcaggaag gtgcgcagcc gcggccgctc
      421 gccgcgcgtc agcggggcca cgatgccgat gccctcggtg atgttgtgga gcgtgaagcc
      481 gatgaccagg aaggtgccca atgccgccgc gccggtggcg aaggccgcgc cgatggccag
      541 cccctcgccc aggttgtgca gcccgatgcc cagcgcgatg agcgtcgata ccgcttgcgg
      601 cgacttctcc cccgaccggc ggccgacggc cagcagcacg cccaggctga gcaggatgcc
      661 gaagatgacc accggcaccc cctggaagac ggccggcgcg gcgtgcgcct gatccagcgc
      721 ctcgagcacc atatccacga agagaaagac cagcaggccg acggtcaggg ccaggatggc
      781 gttcatccat ttgcggtcga tgcggcgcat gaacggatac cacagcatcc ccagcccgac
      841 cgggatgatg gcgacgtaga tgcccagcag ggcgtaagcc agcagcgtgc ggccgtccac
      901 tgccggggac ttcacggcca cggcggccgt cgccgggaag gtgaggccgg tggacgtgat
      961 gagattgatc gccagcggct cgccgttgac ccacgggtag gggatgtcga tggtggccgt
     1021 ctccagccgg tcgagtacgt tgtcggggct gatggcgaac tgccaatagg cgtcgtcgat
     1081 ggtcagttgg gagatggtgg tgcgctccgg gccgccgtta atcacttcca gccggatgct
     1141 gcccggcgcg gggaaggtga tgcgctgcac cgccagcgtc tcgatcggcg gaaacgcgcc
     1201 gcggaaaaac gacagcgggt cgagcgccag aaagccggcg atgagcgccg ccaagatcag
     1261 caaaggcagc agcgccaata cccagcgcgg ggcgtggggt ttggttggtg tggttgttgt
     1321 gtccatagtt tacctctgca ggggagcggg agagccgggg agaaagggag aaagggggca
     1381 ggggagatcc ctttcaccct ttcacccttt ctccctttct ccctttcacc ccttcacttc
     1441 aaaaaacccc atccatccca actccgccag ctccgtctgg tgggcgtgga acatgtagag
     1501 gcccggctcg tgttcggcga agctgaactc caggatgccg cgctgggcct ggcattgcat
     1561 gacggtgtcg atggtcacct gcgtcggccg cagcgtcgtg ccgtggtcgt aatagtcgaa
     1621 aaagttggcg tgcaggtgca tcgagttgat cggatcaaat tcgaggatat taataaggta
     1681 gagacgctgg cgctgctcgc ggcgaatggc gatcggccgc tccatgccgt agccgaaagc
     1741 caccgtgttg gccgcgtaga tttcgttggc gctgtcgaag ttggtgtcga acccgttgag
     1801 aaccatcacc atttcgtgga tcgcctgaca ctccgggtag gtgtggttgc gggcgcgggc
     1861 caatgccgct tcctcgccgc tatatttctc cgggtcgggg tcgatgatga acgcgccgta
     1921 cagccccttg tggatgtggc gcttgagcgg cacggcgtgg cagtggtaga gatggcagcc
     1981 gaacggtttg gcgtcgaact cgtagacggt gcggccgccg ggctggattt ggcccgcgcc
     2041 cacgccgggc acgccgtcca tcgccgccga gtggatgccg tggaagtgga tggtgtgggg
     2101 gtggctgccg ccattgccga actcgatgcg cagccgctcc ccctcgacgc agcgcagcgt
     2161 ggggccgggg cattgcggca ccagcttgcc cgcgcgccgc gatgcggccg aggtcgggct
     2221 gccatagagc cagccgggga acatcacccc cggcgcgatc tcgatctcct tgtccagcgc
     2281 gtaaatctgc caggtgcgca cggtgcggcc gttttcctgc gtcacggtgc catagtcgaa
     2341 gtcggtcaat atctcgtggg ggtcaaagcc gttggcggcg tggtcgacct ggccgatcat
     2401 gcccatgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atctaaataa
     2521 taatatcgag gaggttcaat ttgtcaagca attgtagcgc aagccgccgg cttgcctctt
     2581 ctctttgccc cgcatgcaag ccggcagctt gcaccacatc aggcacaaat gcgatacaat
     2641 cccattcaac gatccggttc tgattctgga gttacccatg tctgatcaac ccgtcaccat
     2701 tctctgtctt tccggctatt tcaagggact gaattttctc atcacggcca aggcgctggg
     2761 cgcgcgcgtg ctgctgttga cgcgcgagaa caacgccgaa gaagactggc cctacgacag
     2821 catcgatcgc ttcatgatca tgcccgacat tcgcaagcgg ccggacatct tccatgccgt
     2881 cagctatctg gcgcgcagtg agtcgatcga cgtcatcgtg ccgctcgacg actacgatgt
     2941 ggagacggcc gccgcgctgc gcgagcattt gcgcttgccc ggtctgggcg agacacaggc
     3001 gcgctacttc cgcgacaagc tggccatgcg cctgcaggcc gccgccgccg gcatccctgt
     3061 gccccccttc acgccgacgt tcacctatga ccggctgcgc gagtacatgg agcgcgtgcc
     3121 gccgccgtgg gtgctgaagc cgcgctccga ggccggggcg atgggcatca agaagatgta
     3181 ttcggccgag gaagtctggc ggcggctcga tgagctgggc gacgagcaat cgaacttcca
     3241 tctggagcag ttcgtccccg gcgaaatcta tcacgtcgat tcgctggtct acgatggcga
     3301 gctgctcttt gcccagccgc acaagtacgc gcggccgccg ctgaccgtct cccacgacgg
     3361 cggcgtgttc gccagccgca cactgcccga cggcgaggaa gcgcgggcca tcctcggcct
     3421 caacgcgcgg ctgctgcgcg cctttgggct gcagcgcggc gcggcccatg ccgagttcat
     3481 ccgcggcgcc gacgggcagt tctactttct ggaagtggcc gcgcgggtgg gcggggccaa
     3541 catcgaaaac ctgatcgagg cggccaccgg tctgaatccg tggggcgaat gggcgcgcat
     3601 ggagatcgcc tatgcccagg ggcagcccta caccgtcagc cccacgcgcc acgtttatgc
     3661 cggtctgctt gtctgtctgg cgcggcagga gtggcccgat ctgtcgggtt acaatgaccc
     3721 cgaagtggtc tatcgggtgc acaagaagca gcacgccggg ctggtcgtcg cttcgcccga
     3781 tccgggccgg gtagagtggc tgctcaatca atatgccgag cgtttcggcc gcgacttttt
     3841 ggcggtgctg cccgcgctgg ataaagcgcc gctttgagtg gtgagtgacg aacgacgagt
     3901 gacgagtgaa gagtatcgtg gtcagtggtc agctggtcgc tcatcttcac tcgtaactcg
     3961 taactcgtaa atttaagaat cggaggatat catgggactt gaattgttgt gtgcggttgt
     4021 tattgcgttg ggcttcggcg tgttggtggc cttcagcggc tatcgcctct tcctgagcct
     4081 gctccccatc tggggcttct tcttcggctt cgtgctgggc acgcagacca tccaggcgct
     4141 catcggcgac ggcatgttcg ccacggcgac cagctgggtg gtgggctttg tggtggccct
     4201 ggtcttcgct gtgctgtcct atctgtttta tttctgggcc gtggccgtcg ttgccgggtc
     4261 gctgggctat ggcgtcggtg tcggcctgat gaacctgatc ggcctggatt tcgcgctcat
     4321 cccctggata gtgggcatca tcctgggcgc cgtcctcatc gtcgtcacct tccgctataa
     4381 cctggccaag gtggtgatca tcgccgccac agccgtgggt ggtgcggcgc tggccatcgg
     4441 tacgctggcg ctggggccgg ggaacgtctc cctgggcagc gccttcaaca atccggttca
     4501 ggcggtgctt ggcctcggct ttctctgggc catccttttt gtgatcatgg ccgccgccgg
     4561 attctacgtc caatggcaag ccaatcgcgc ctggacggcc gaaccttacc ccaaccgcat
     4621 ctaaccaatc catcacccac cagggcggca ggtccacgat cccactcacc cctgccgcct
     4681 tttccctctt ccttacatca tttatacgac cgccgaccag tagatcagta ttcagtaggg
     4741 cagtattcag ttcactgacc cactgaccca ctgacccact gacccactga cccactgacc
     4801 actgacccac tgattttaca gattctttac gcctttcccc gttttccctg acattttcct
     4861 g
//
LOCUS       DAOMQG010000264         4820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7379_length_4820_cov_6.534732, whole genome shotgun sequence.
ACCESSION   DAOMQG010000264 DAOMQG010000000
VERSION     DAOMQG010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_4820_cov_6.534732"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..746
                     /locus_tag="PLC06_12595"
     CDS             45..746
                     /locus_tag="PLC06_12595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRO91331.1"
                     /translation="MNETRFEGVIFDFNGVLLWDNHLHEEVWRDLSARMRGTAMTMAE
                     MHEAVHGRVNRDIFEYVLNRPLGDAELQSLVDEKESLYRQLALDNLDTYYLAPGAVDL
                     LDFLAANGIKRAIATSSPKVNVDFFIEHLDLYRWFTPETIVYDLGRYPGKPAPGIYLE
                     AAERLGSPPERLIVAEDAASGIQAAYSAGAGAIVAVGPADSRERLAALPGVTMVVEDM
                     TGFPRSLLLAAVRNS"
     gene            complement(770..2140)
                     /locus_tag="PLC06_12600"
     CDS             complement(770..2140)
                     /locus_tag="PLC06_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HRO91332.1"
                     /translation="MLTGYPRNAPPRFHVLVKPTGATCNLDCAYCFFLSKEMLYPGSR
                     FRMADDLLETYIRQLIEGHQSREVTLAFQGGEPTLMGVPFFRRAVELAEKYRRPGMTI
                     EYTIQTNGTLLDDEWGEFLRANNFLVGISIDGPREIHDLYRYDKGGAPTFDRVMRGLD
                     ALKRHKVDWNVLCTLHRNNAGKPLELYHFFRDELGAQFMQFIPIVERLTPEQAAVSMS
                     LVDPWHSWRDRPLYTQSGTVVTERSITGEQYGAFLIAIFDEWVRRDVARVYVQMFDVA
                     LANWLGMPSGLCIHSRTCGTALAIEHNGDLYSCDHFVEPQYFLGNIKQEHLIDLVASE
                     QQVKFGNDKFDTLPSYCRECDVRFACHGGCPKDRFITTPDGEPGLNYLCAGYKAFFHH
                     IRGAMDYMVNELKHERPPANIMAALARSDAAREAAQSVGRNAPCPCGSGKKFKHCHGA
                     TKARQG"
     gene            2245..2991
                     /gene="trxA"
                     /locus_tag="PLC06_12605"
     CDS             2245..2991
                     /gene="trxA"
                     /locus_tag="PLC06_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRO91333.1"
                     /translation="MATSRIMEVDQANFDRDVLQRSADELVVVDFWAPWCGPCRMLGP
                     ILERVAGEPDAGFILAKVNTDRNPTLAGRYDIRGIPAVKAFRNGKVVDEFVGVRPEPA
                     VREFIRANGGGSRRPPEPPPGDPAERLRRARELLARGAGCAAADLLAESGGPSADAAA
                     RLRPLADYLCRASRGDQFSTAKNAAADAHRQAVDAWNRKEPSAALYSLLAAYNQESGA
                     DKARTKAVMEGIFEMLGAESVVTRQYAGYL"
     gene            3002..3301
                     /locus_tag="PLC06_12610"
     CDS             3002..3301
                     /locus_tag="PLC06_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRO91334.1"
                     /translation="MFTHVVLFRLKDRRPESVAASRAVLESLAGGIETLRGLEVGTDI
                     VHSERSYDLALITRFDDMAGFETYRQHPVHLPVLAHLKEAVESSVAVDFVEGEKG"
     gene            3412..4275
                     /gene="pyrF"
                     /locus_tag="PLC06_12615"
     CDS             3412..4275
                     /gene="pyrF"
                     /locus_tag="PLC06_12615"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRO91335.1"
                     /translation="MRYLEKLRAAQEMNQSWLCVGLDPQPEFMPVEAAKWDEPVLPFN
                     RRIIEATADLVCAYKPNLGFYLQWGAAGVIALERTIAVIPDHIPIILDAKIGDIGNTQ
                     EAWGRGLFDEWGVDAITVNPYVGADAVRPLLAGRPAKAVYVLARTSNPGAPQFQGDLT
                     AGDGLSAAVLREAQNWAGPDDGHCGFVVGATWPGELAAARKLAPTANFLIPGIGAQGG
                     DLAAAVAHGLPVGQGADSRVGPVINAGRSVIYASAGLDFDEMARAAALKLRDEINELR
                     NSGSLRQSTIK"
     gene            4325..>4820
                     /locus_tag="PLC06_12620"
     CDS             4325..>4820
                     /locus_tag="PLC06_12620"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRO91336.1"
                     /translation="MFFFDPVYMLYVFIPTMLLSLGVQLYLKRSYAKWSKVRNSTNLT
                     GTQVGEALFQRTNLMNIPLEVVPGQMTDHFDPRANVVRLSQGTASQPSVAAMAIAAHE
                     LGHVQQYQTGSALIKARNFLVPAVQFSPMISYAAIMIGLILNITGLFWVGIAFFGVMV
                     FFTIL"
BASE COUNT          831 a         1502 c         1596 g          891 t
ORIGIN      
        1 gcccacctgt caggtcttga agctgttagt gagagtgaac gattatgaat gaaacgagat
       61 tcgagggcgt cattttcgac ttcaacggcg tcctcttgtg ggacaatcac cttcacgagg
      121 aggtgtggcg cgatctgtct gcccggatgc gcggcacggc gatgaccatg gccgaaatgc
      181 acgaggccgt ccacggccgc gtcaatcgcg acatcttcga gtacgtgctg aatcggccgc
      241 tcggcgacgc cgaactccag tcgctggtcg acgaaaagga gtcgctctat cgccaattgg
      301 cgctggacaa cctcgacacc tactacctcg cccccggcgc ggtggacttg ctcgattttc
      361 tggccgccaa cgggattaag cgggccatcg ccacctcgtc gcccaaggtg aacgtggatt
      421 ttttcatcga gcatctggac ctctatcgct ggtttacgcc ggagacgatt gtctatgacc
      481 tcggccgcta ccccggcaaa ccggctcccg gcatctacct ggaagcggcc gaacggctgg
      541 gcagcccgcc ggagcgtttg atcgtcgccg aggacgcggc gtcgggcatc caggctgcct
      601 atagcgcggg ggcgggggcg attgtcgccg tcggcccggc cgattcccgc gagcggctgg
      661 ccgcgctgcc cggcgtgacg atggtcgttg aggatatgac cggctttccg cgatcgctgc
      721 tgctcgccgc tgttcgtaat tcgtaattcc tcgctacaag cgaagagcgc tacccctgcc
      781 ttgcttttgt ggccccgtgg caatgcttga acttcttgcc gctgccgcag gggcacgggg
      841 cgttgcggcc gacggattgg gccgcttccc gcgcggcgtc gctgcgggcc agggcggcca
      901 tgatattggc cggcggccgc tcgtgcttca gctcgttgac catgtagtcc atcgcgccgc
      961 ggatgtggtg gaaaaacgcc ttgtagcccg cgcacagata attcagtccc ggctcgccgt
     1021 cgggggtggt gatgaagcga tccttggggc agccgccatg gcaggcgaag cggacgtcgc
     1081 actcgcggca atagcttggc agcgtgtcga acttgtcgtt gccgaatttc acctgctgct
     1141 ccgaggcgac caggtcgatc agatgctcct gcttgatgtt gcccaggaaa tattgcggct
     1201 cgacgaaatg atcgcacgag tagagatcgc cgttgtgttc gatggccagc gcggtgccgc
     1261 aggtgcgtga gtggatgcac agccccgacg gcatgccgag ccagttggcc agggcgacgt
     1321 cgaacatctg cacatagacg cgggccacgt cgcggcgcac ccattcgtcg aagatggcga
     1381 tcaggaacgc gccgtactgc tcgccggtga tcgagcgctc ggtcacgacg gtgccgcttt
     1441 gggtatagag cggccgatcg cgccaggagt gccacgggtc gaccaggctc atcgaaacgg
     1501 ccgcctgctc cggcgtcaga cgctccacga tggggatgaa ctgcatgaac tgcgcgccca
     1561 gctcgtcgcg gaagaagtga tagagttcca gcggcttgcc ggcgttgttg cggtggagcg
     1621 tgcacaggac gttccagtcg accttgtggc gcttgagggc gtcgaggccg cgcatgaccc
     1681 ggtcgaaggt cggcgcgccg cccttgtcat agcgatacag gtcgtggatc tcgcgcgggc
     1741 cgtcgatgct gatgccgacg aggaagttgt tggcccgcag aaattcgccc cactcgtcgt
     1801 cgagcagggt gccgttggtc tggatggtgt actcgatggt catccccggc cgccggtact
     1861 tctcggccag ctccaccgcc cgccggaaaa acggcacgcc catcagcgtc ggctcgcccc
     1921 cctggaaggc cagcgtcacc tcgcgggatt gatgcccctc gatgagctgg cggatgtagg
     1981 tctccagcag atcgtcggcc atgcggaagc gactgccggg atagagcatc tctttcgaca
     2041 ggaagaagca gtaggcgcaa tcgaggttgc aggtggcccc ggtcggcttg accagcacat
     2101 ggaagcgggg cggggcattg cgcggataac cggtcagcat cgtcatgata caatgataat
     2161 aggcaataat cttgaaaact caaccaagtg ggttatcctg tgggcagacc aaatgctgcc
     2221 gtcaatcggc aagggggttt aatcatggca acaagtcgaa ttatggaagt cgatcaggcc
     2281 aactttgacc gggacgtgct ccagcgctcg gccgacgagt tggtcgtggt cgatttttgg
     2341 gcgccgtggt gtggcccgtg ccgcatgctc ggccccatcc tggagcgcgt ggccggtgag
     2401 ccggacgcgg gcttcatcct ggccaaggtc aacaccgacc gcaacccgac gctggccggc
     2461 cgctacgaca ttcgcggcat cccggcagtg aaggcatttc gcaacggcaa ggtcgtcgat
     2521 gagttcgtcg gcgtgcggcc ggagccggcg gtgcgcgagt tcatccgcgc caatggcggc
     2581 ggctcacggc ggccgcccga acccccgccg ggcgaccccg cggagcgctt gcgccgcgcc
     2641 cgcgagctgc tggctcgtgg tgcgggctgc gccgcggccg acttgctggc ggagagtggt
     2701 ggcccctcgg ccgatgcggc cgcgcggctg cgcccactgg ccgattatct ttgccgcgcc
     2761 tcgcgtggcg atcaattctc taccgccaaa aatgccgccg ccgacgccca ccgccaggcc
     2821 gtcgatgcct ggaaccgcaa ggagccgtcg gccgcgctct acagcctgct cgccgcctac
     2881 aatcaggagt cgggagccga caaggcgcgc acgaaggcgg tcatggaagg gattttcgag
     2941 atgctgggcg cggagagcgt cgtcacccgt caatatgcgg gctatctata ggagccatta
     3001 tatgttcacc cacgtggttt tgtttcgcct gaaagatcgt cgcccggaga gcgtggccgc
     3061 gtcgcgggcg gtgctggagt cgctggccgg cggcatcgag acgctgcgcg ggctggaggt
     3121 gggcacggat atcgtccatt ccgagcggtc gtatgacctg gcgctcatca cccgcttcga
     3181 cgacatggcc ggcttcgaga cctatcgcca acaccctgtc cacctgccgg tgctggccca
     3241 tctgaaggaa gcggttgaga gttcggtggc ggtggatttt gtggaagggg agaaggggtg
     3301 atagggggaa gggggggaag gggagatgaa gggacgggcc ggtgatctgc cgtccgccgg
     3361 ttgttgctca tgatcggtgg gtgctcaatc taatcaaaaa ggactggtga catgcgttat
     3421 ctggaaaaat tgcgcgcggc gcaggagatg aatcaatcgt ggctgtgcgt cgggctggac
     3481 ccgcagccgg agttcatgcc cgtcgaagcg gccaagtggg atgagccggt gctgccgttc
     3541 aaccggcgca tcatcgaggc cacggccgat ctggtctgcg cctacaagcc caacctcggc
     3601 ttctatctgc aatggggcgc ggccggggtc atcgctctgg agcgcaccat cgccgtcatc
     3661 cccgaccaca tcccgatcat cctcgacgcc aaaatcggcg acatcggcaa cacccaggaa
     3721 gcctgggggc gagggctgtt tgacgagtgg ggggtggacg ccatcaccgt caacccctat
     3781 gtcggcgccg acgcggtgcg gccgctgctg gccgggcgcc ccgccaaggc ggtctacgtg
     3841 ctggcccgca cctcgaaccc cggcgcgccg cagttccagg gcgacctgac cgcgggcgac
     3901 ggcttgtcgg ccgccgtgct gcgtgaggcg cagaactggg ccgggcccga cgacggccat
     3961 tgcggcttcg tcgtcggcgc gacctggccg ggcgaactgg ccgcggcccg caagctggcc
     4021 cccaccgcca acttcctcat ccccggcatc ggcgcgcaag ggggcgacct ggccgcggcc
     4081 gtcgctcatg ggctgcccgt tggccaggga gccgacagcc gggtcgggcc ggtcatcaat
     4141 gccggacgca gcgtgatcta cgcctcggcc gggcttgatt tcgacgagat ggcccgcgcc
     4201 gcggcgctca agctgcgcga cgagattaac gaactgcgga attcgggatc attgcgacaa
     4261 tcaacgataa aataacgtat aattttcatc acaaatctga ttatttgagc gaggagtgct
     4321 cgatatgttc tttttcgatc cggtctacat gctttacgtg ttcataccca cgatgctctt
     4381 gtcgctgggc gtgcagctct atctgaagcg gtcgtatgcc aaatggagca aggtccgcaa
     4441 cagcaccaat ctgaccggca cccaggtggg cgaggcgctg ttccagcgca cgaacctcat
     4501 gaacatcccg cttgaggtcg tgccgggcca gatgaccgat cacttcgacc cgcgagccaa
     4561 cgtcgtgcgc ctgtcgcagg ggacggcctc gcagcccagc gtcgccgcca tggccatcgc
     4621 cgcccacgaa ctgggccatg tgcagcagta ccagaccggc tcggcgctta tcaaggcccg
     4681 caacttcctg gtgcccgccg tgcagttcag cccgatgatc tcctatgccg ccatcatgat
     4741 cggcctaatt ctgaatatca ccggtctgtt ctgggtcggc atcgccttct tcggggtcat
     4801 ggtcttcttc accatcctga
//
LOCUS       DAOMQG010000265         4815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7388_length_4815_cov_5.194538, whole genome shotgun sequence.
ACCESSION   DAOMQG010000265 DAOMQG010000000
VERSION     DAOMQG010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7388_length_4815_cov_5.194538"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(434..4501)
                     /locus_tag="PLC06_12625"
     CDS             complement(434..4501)
                     /locus_tag="PLC06_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91337.1"
                     /translation="MTRNARIRLFVLCVVCLMIVPVVAAAQTPGHDADRPPVAQAVSL
                     PGATPVTRDVEDQILDPWPSSWLPESAPVSPPAGEGPAVPPPDDRAAGGHQDEGDWGR
                     VTYHAYDRDRGQYDLFVMRGLQAGQGSNRLTNDAAVDFQPALSPDGTRLAFVSAPARS
                     DGTFEDLEIYVASFNYVTGSMGPPTRLTDNDTNDYWPVWSPDGKRLAFYRILGQQSDV
                     FVMNADGSGQTNITNHPAGDAFPAWSPDGQQIAFSSTRTGEFRIHVMSAGGSNVRQLS
                     NQRLSLRPVWSPDGTKIAYSADSTGDSWWDLMVMDADGKNQVTMKPTVPLTDIEVRSW
                     APNGKTITFTRTEYFLYQGDLYLTAATLWAYDYFPGQPWRDREYQLGYGNTVFEPHWG
                     TIDGRPPQTTVAALPAESPATFTIRWDGADRGVAGLNTFDVQMQINGGPWVDLKPYEG
                     FTSMDIEGVGGQQLAFRSRGRDFAGNVEPWPSTPNTKTMVENRPPVTTMGPLSPFTRA
                     GDPVAIGWTSYDPGGSNIKEEELNFSINNGPWKKLEFYIAEPGYVFLPDEHGVKPGDR
                     VGFRVRSIDYALNVEPWTPDPGDGLTTYFSSRAVGRAVDNTGNPVGGAAVAANPAGLM
                     TVATAADGRFMVHMGAVVPSLSLEWAKPGYGALPPATRVMPTDRGVSPVMPPAVDALI
                     NGNFEAAGWGAWQPGGSIAPNRLNDAEQAHTGTAAAGLGARAASFGLPIRVKTTPDVI
                     EWQLVMAIDGEGRAFVVWASGDSGPLSSATRRPDGTWTNVVQLGSGMRNSQLITAPDG
                     QVWLTAVSGTVRLWRQSGEGWAGSEQIPGTEEVNQFSLIAGPGGRLDFLWCGREGAVN
                     YIRRSGGVWSAKTQIGGGRPDMGWCEFEMATTPDGSVHVIWAEPDGGISGDHHTVYRR
                     RAPDGSWGAVTILHEGGPGSLVSDAGGRVHLIRPGYEPNNFGKLAWQYRQWNEGVWGL
                     EETLGFYKNPNLLSKVMASADGAVQMAMSEGGAQFFLRREPDGRVTEESLPAGYGYRS
                     TMTLDAMGGAHFAYPTSDQHGVGSLFYLTRSPDGDWIGPVNVAKATAETQVSWEAPGL
                     AVDAQGNPYAAWLQYGPAVIPGFTQPDVAFAGPALAANGGESILSQTVAVPAGMVHPT
                     LSFVYQSRGPLRLELAKPDGTPLLTRELPSSPTGMRHEWLDMSTYKGQSVKITFRQAQ
                     VAGKPVGWAVVDDVSLGRAHADIAVWGESGGRRPNNTMIHTLTVANPGALAAGGVALV
                     YDLPAELTFVSAQPAPAGTSPLRWTLGALAAGAQQTITITLAVPRQGTPPWVSATAEI
                     TTADEELELLNNSAEVVSGLGRSVILPVVLRPDDD"
BASE COUNT          878 a         1574 c         1505 g          858 t
ORIGIN      
        1 gcgacgcggt cgcggtcggc caggcagcgg atggtatagt aggtctgggc gctggcggca
       61 tgggtcatgg cggccgccag gcgggccgtg gatggatcgc gagtggcaga agtggaatcg
      121 aggcttcccg agagagcaga cattgcatca ctcattgggc tgctgggatc gcgcccgact
      181 gattcggcgg cgggctatcg ccctttcgca ccaatgaaag aataaaacaa tgtcgcttgt
      241 ggcggatagt gacaaaaatc acatattaag gtgatcgatg taataagcgt ctgatgggat
      301 ttacccgtga cctggatgtg ctgttggcgc aatggtcgag cgccgcttcg cgcgcggccg
      361 cgatctcgtc gctggaatac ggcgcgacac ggacggcgac tcctctcgtc accgcccgtg
      421 cctctcgact atctcaatcg tcgtccggcc gcaagacgac cggcagaatc accgaacgcc
      481 ccagccccga gaccacctcg gccgaattgt tcagcagctc cagctcctca tcggcggtgg
      541 tgatttcggc cgtggccgac acccagggcg gcgtcccctg ccgggggacg gccagtgtga
      601 tcgtgatcgt ctgctgcgcc ccggcggcca gcgcgcccaa cgtccagcgc agcggcgacg
      661 ttccggccgg cgcgggctgg gcggagacga aggtcagctc ggccggcaga tcatagacca
      721 gcgccacccc gccggcggcc agcgcgccgg ggttggccac agtcagggtg tggatcatcg
      781 tgttgttcgg ccgccggccg ccgctctcgc cccaaacggc gatgtcggcg tgggcgcggc
      841 ccaggctgac atcatcgacg accgcccagc ccacgggctt gccggccacc tgcgcctgcc
      901 ggaaggtgat cttcaccgac tggcctttat acgtcgacat gtcgagccac tcatggcgca
      961 tgccggtcgg gctcgacggc agctctctgg ttagcagggg cgtgccgtcg ggcttggcca
     1021 gctccaaccg cagcgggccg cgcgactggt agacgaacga cagcgtcggg tgaaccatgc
     1081 cggcgggcac ggccaccgtc tgcgacagga tcgattcgcc gccattggca gccaacgccg
     1141 gcccggcaaa agccacatca ggctgcgtga agcctgggat tacggccggc ccatattgca
     1201 gccaggccgc ataggggttg ccttgcgcgt ccaccgccag cccgggagcc tcccaggaaa
     1261 cctgcgtttc ggcggtagcc ttggccacat tgaccggccc aatccagtcg ccatccggcg
     1321 agcgcgtcag atagaagaga ctgcccaccc catgctggtc gctcgtcgga taggcaaagt
     1381 gcgccccgcc catggcgtcg agagtcatgg tggagcggta cccatagccg gccggcaacg
     1441 actcctccgt cacccggccg tccggttcgc gccgcaaaaa gaattgcgcg cctccctccg
     1501 acatcgccat ctgcaccgcg ccgtcggccg acgccatcac cttcgaaaga aggttggggt
     1561 ttttgtagaa gcccaatgtt tcctctaacc cccatacgcc ctcattccat tgccgatatt
     1621 gccatgccaa tttgccaaag ttgttcggct catagcctgg ccggatgaga tggacgcggc
     1681 cgcccgcatc cgataccaga ctgcccgggc caccctcgtg aagaatagtg actgcccccc
     1741 aggagccgtc aggggcgcgc cgccgataaa cggtgtggtg gtcgccactg ataccgccgt
     1801 ccggttcggc ccagatgaca tggaccgatc cgtcgggcgt cgtcgccatc tcaaactcac
     1861 accaccccat atccggtctg ccaccgccga tctgcgtttt cgccgaccag accccgcccg
     1921 accgccggat gtaattgacc gcaccctccc gaccgcacca gaggaagtcc agtcggccgc
     1981 ccggtccggc tatcaggctg aattgattca cctcctcggt tccggggatc tgctcactcc
     2041 ccgcccaacc ttcgccggat tgacgccaca gccggacggt cccactcacg gccgtcagcc
     2101 agacctggcc atcgggcgcg gtgatcagct ggctatttcg catcccgctg cccagttgga
     2161 cgacgttggt ccacgtgccg tcggggcgac gggtagcgct gctgagcggt ccagagtcac
     2221 cggatgccca aaccacgaag gctcgaccct cgccgtcgat ggccatcacc agctgccatt
     2281 cgatcacgtc gggagtcgtc ttcacccgaa tcggcaagcc gaaggaggcg gcgcgcgcgc
     2341 ccagcccggc ggcggccgtt ccggtgtggg cttgttcggc gtcgttcaac cggttggggg
     2401 cgatggagcc gcccggctgc cacgctcccc agcccgcggc ctcaaaattg ccgttgatca
     2461 gcgcatcgac ggccggcggc ataaccggac tgacgccgcg atcggtcggc atcactcgcg
     2521 tggccggcgg cagcgcgcca tagcccggct tggcccattc cagcgacagc gacgggacga
     2581 ccgctcccat atggaccatg aatcggccat cggccgcagt tgccacggtc atcaggcccg
     2641 ccggattagc ggcgaccgct gcgccgccca ccgggttgcc ggtattgtcg acggcgcggc
     2701 cgaccgcccg acttgagaag tatgtcgtga gtccatcgcc aggatcgggt gtccaaggct
     2761 caacattgag ggcgtaatcg atgcttcgca cccgaaaacc caccctgtct cccggcttca
     2821 cgccatgctc gtcaggcaga aaaacgtagc ccggctcggc aatatagaac tccagttttt
     2881 tccagggccc gttgttgatg ctgaaattca gttcttcttc cttaatattc gatccgccgg
     2941 ggtcatagct ggtccagccg attgccaccg gatcgcctgc ccgcgtgaaa ggtgacagcg
     3001 gccccatggt agtgaccggc gggcgatttt ccaccattgt tttggtgtta ggggtgctcg
     3061 gccagggttc aacgttgccg gcgaaatcgc gaccgcggct gcgaaaggcc agctgctgcc
     3121 ccccaacgcc ctcgatgtcc atcgaagtaa acccctcgta aggcttcaaa tcgacccacg
     3181 gcccgccgtt gatctgcatt tgcacgtcga atgtgttcag cccggccacg ccgcgatccg
     3241 cgccgtccca gcgaatggtg aaggtcgccg gagattcggc cggcagcgcg gctacggtcg
     3301 tctgcggcgg ccggccatca atggtgcccc aatgcggctc gaaaacggta ttcccgtagc
     3361 ccagctgata ttcacgatcc ctccacggct ggcccggaaa atagtcataa gcccacaggg
     3421 tggcggccgt tagatagaga tccccttgat acaagaagta ctcggtccgc gtaaaggtga
     3481 tggttttgcc gtttggcgcc cagcttcgca cctcgatatc ggtcaacggc actgtgggct
     3541 tcatggtgac ctgattcttg ccgtcggcgt ccatgaccat caaatcccac cagctgtccc
     3601 cggtagaatc ggcgctgtag gcgattttcg tcccgtcggg cgaccagacc gggcgcaagc
     3661 tgaggcgctg gttcgatagc tgccgcacgt tgctgccgcc ggcgctcatg acatggatgc
     3721 gaaattcgcc cgtgcgggtc gacgaaaaag caatttgttg gccgtcgggc gaccaggcgg
     3781 gaaacgcgtc ccccgccgga tgattggtga tgttggtttg gccggagccg tcggcgttca
     3841 tgacgaagac gtccgattgc tggccaagga tgcggtagaa ggccaatcgc ttgccgtcgg
     3901 gcgaccagac cggccaataa tcattggtgt cgttgtcggt cagccgggtg ggcggcccca
     3961 tggaaccggt gacgtagttg aatgaggcga catagatctc gaggtcttca aaagttccgt
     4021 cgctcctggc cggggcggag acaaaagcga ggcgcgttcc gtcggggctg agggccggct
     4081 ggaagtcgac ggccgcgtca ttggtcagtc ggttgctgcc ctgtcccgcc tgcaggccgc
     4141 gcatgacgaa gagatcatac tgacctctat ctctatcata ggcatgatag gtgacgcggc
     4201 cccaatcgcc ctcatcctga tggccgccgg cggcgcggtc atcgggtggc ggcacggcgg
     4261 ggccttcgcc ggcaggcggc gataccggcg cgctttcggg caaccaggac gatggccagg
     4321 ggtcgaggat ttggtcttcg acatcgcggg tgacgggcgt cgcccccggc aagctgacag
     4381 cttgcgccac aggcggccgg tcggcgtcat ggccgggggt ttgtgccgcg gccacgaccg
     4441 gaacaatcat caggcaaaca acacacagga caaaaagccg gatacgcgcg ttgcgagtca
     4501 tggattgtca ctcccggggg aatctggtaa agagggtaat cgcccccccc cgaggcaatt
     4561 atgggcagta tagcaaaccc gatgaccatt ctcaataggt gcaatgacgg attccaaata
     4621 taggcggatc cgccgcacta tcccgcccgg ccatcccgtg gtaaaataga atcatggtca
     4681 ccttgatcgt ccccgaacga cggcacacga agcgcaagct gtcctatgag gagtatctcg
     4741 ccctgcccga cgacggccgc attgtggaat gggtcaatgg agaaattatt acccatatgc
     4801 cggcaacatc attac
//
LOCUS       DAOMQG010000266         4803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7418_length_4803_cov_9.404170, whole genome shotgun sequence.
ACCESSION   DAOMQG010000266 DAOMQG010000000
VERSION     DAOMQG010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_4803_cov_9.404170"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..315)
                     /locus_tag="PLC06_12630"
     CDS             complement(31..315)
                     /locus_tag="PLC06_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HRO91338.1"
                     /translation="MEFEFSGKMWFWKGPAPWHFITVPAELGLDLNAMASALTYGWGM
                     IPANVLIGDTEWRTALWPRDGSYIVPIKAAVRKLEDLKVGDQATVHLEIG"
     gene            520..2262
                     /locus_tag="PLC06_12635"
     CDS             520..2262
                     /locus_tag="PLC06_12635"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRO91339.1"
                     /translation="MSENTRNLSNLLAGWAAAVRDTAHPQPPAYHGPDVAITRFTEKT
                     SEVEPGAAFVARVRTGSDGHPWIGRAIAGGASLILAQRSPAELGLVIPPGVGWLLVDD
                     TAEALAWLSAAWEGFPSRELVVIGITGTDGKTTTANILFAILRAAGVRAGLLSTIKAD
                     IGGAEELLELHVTTPESPVVQRYLRRMADAGLTHCVLETTSHALAQHRVAAVDFDMAV
                     VTNITHEHLDYHGGYEAYFAAKRHLFEYLLQDIIHVPTTNRRKVELRRTAVLNRDDGS
                     FARLDAFLAGRPVDVARYGLGDGQAPADDETPAATASDIAYGPQETALTFHLAGERPL
                     AVRAPLPGAFNVYNMLAAAAAARALGVDGEAIRAGIEGLTYLSGRMERIDRGQPFLVV
                     VDFAHTPNALDRAIAAARGMIQGKGEGELGSRGAGEPENRDDLPFPPSPEGRVIAVFG
                     SAGKRDVEKRRLMADISARAADLTVLTAEDPRTESLDDILAMMAGAATAAGGVEDETF
                     WRVPDRGRAIYLALELAQPGDVVLICGKGHEQSMCFGTVEYPWDDRTATAAALDAWRE
                     GRPMGDLGLPTFGE"
     gene            complement(2334..3635)
                     /locus_tag="PLC06_12640"
     CDS             complement(2334..3635)
                     /locus_tag="PLC06_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRO91340.1"
                     /translation="MRKKTTAQPKYEVRSTKKPRLSYFVLRTSYLLLALITLLVAGAA
                     AGGPAERPAAVASGGGEYVLPTADRMSDAERAEIEAMLAANTARLSATGLLPAPDPRA
                     SAQVTLDWPLRPRAGFADPGYHAVSGFMDHNPAFPNQRLDYACGGRTYDSPSGYNHQG
                     TDLFLWPFAWNKMDAGDVEVVAAAPGVIIGRSDGNFDRSCTLNGNKWNAVYVYHADGS
                     VAWYGHLKRDSLTPKGVGATVAAGEYLGTVGSSGNSTGPHLHFELYDNGTLTDPYVGE
                     CNQLPGGSWWIQQRNYYDSAINRLATGTKAPTWGSCPNPDTPNEATQFQPGDRVTFLA
                     YYRDLVAGKPTAFRIIQPDGSIFTQWSHAIAQPHYTTSYWWWAYNLPADAPAGAWQFE
                     ADFNGTTHRHTFHVGPAVTPTPTPIAPAWKGFVPIIMASDE"
     gene            4173..>4803
                     /locus_tag="PLC06_12645"
     CDS             4173..>4803
                     /locus_tag="PLC06_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91341.1"
                     /translation="MIDEKTVWDDIWPVVEQLISATVAEDSRTLRQLLYPHGQAADAL
                     ELFGPVVFDILLKTVLGRDRLGLTRAIEGDGGETAFIEYAWPDPATQGSYTAVDVVAV
                     RLAHGDAGWRVVEINPAAADLPLNSMRATSILAGMQVMNEDGKLPSEPWVLPVALYAG
                     VLQLPLQPTAAADPVEALLLPGLQARGFGFLPQMLARRLWRDFVAAASAG"
BASE COUNT          778 a         1473 c         1677 g          875 t
ORIGIN      
        1 ggtatctgtc aaatgccgtg tcggggagtc tcagccgatt tccagatgca ccgtcgcctg
       61 gtcgccgact ttgaggtctt ccagcttgcg gacggcagcc ttgatgggca cgatgtagct
      121 gccgtccctg ggccacaggg cggtgcgcca ttcggtgtcg ccgatgagca cattggcggg
      181 gatcatcccc cagccgtagg tcagggcgct cgccatggcg ttcaagtcga ggcccaattc
      241 ggcgggcaca gtgatgaagt gccagggggc ggggcctttc cagaaccaca ttttgccgct
      301 gaattcgaat tccatgtgcg tattatagcc gaatcccggt cataagatcg tcaattaagc
      361 gctttgctcg taggtcagcg cttcgttcgt agtccagcgc tttagcgcgg ctcttctctt
      421 gagtcatgca gaacagcgct gaagcgctta actacaaaca aagccgctct ccactcgtac
      481 atgcataccg gccgattgct gctactattg atgggtgtta tgagcgagaa cacccgtaac
      541 ctgagcaacc tgttggccgg ctgggcggcg gccgtccgcg ataccgccca tccccaacca
      601 ccggcctacc acggccccga cgtcgccatc acccgcttca cggagaagac cagcgaggtg
      661 gaaccgggcg cggcctttgt cgctcgcgtg cgcaccggca gcgacggcca cccgtggatc
      721 ggccgggcca ttgccggcgg cgcgagcctg attctggccc agcgctcccc ggccgaactg
      781 ggcctggtca ttccgccggg cgtcggctgg ctgctggtcg atgacacggc cgaggcgctg
      841 gcctggctgt cggccgcgtg ggagggcttc cccagccgcg aactggtggt catcggcatc
      901 accggcaccg acggcaagac gacgacggcc aacatcctgt tcgccatcct gcgcgccgcc
      961 ggggtgcgcg ccgggctgct cagcaccatc aaggccgaca tcggcggggc cgaagagctg
     1021 ctggagctgc atgtgaccac gccggagtcg ccggtggtgc agcgctattt gcggcggatg
     1081 gccgacgcgg ggctgaccca ttgcgtgctg gagacgacgt cccatgcgct ggcccagcat
     1141 cgggtggcgg ccgtcgattt cgacatggcc gtcgtgacca atatcaccca tgagcatctg
     1201 gactaccacg gcggctatga ggcgtatttc gccgccaagc gccacctgtt cgagtacctg
     1261 ctgcaggaca tcatccacgt gccgacgacc aaccggcgca aggttgagct gcggcggacg
     1321 gcggtgctga atcgcgacga cggctctttt gcgcggctgg atgcgtttct ggccgggcgg
     1381 ccggtggatg tggcccgcta tgggctgggc gatggccaag cgccggccga cgacgaaacg
     1441 ccggctgcga cggcgtcgga tatcgcctat gggccgcaag agacggcgtt gacgttccat
     1501 ttggccgggg agcggccgct ggcggtgcgc gcgccgctgc cgggggcgtt caatgtctac
     1561 aacatgctgg cggcggcagc ggcggcgcgg gcgctgggcg tcgacggcga ggccattcgc
     1621 gcggggatcg aggggctgac gtatctcagc gggcgcatgg aacgcatcga tcgcggccag
     1681 ccgtttctgg tggtggtcga ttttgcccac acgcccaacg cgctggatcg ggcgattgcc
     1741 gcggcgcggg gaatgattca gggaaaggga gaaggggagt tggggagcag gggagccgga
     1801 gagccggaga accgggacga tctccctttc cccccctctc cagagggtcg cgtcatcgcc
     1861 gttttcggca gcgcgggcaa gcgggatgtg gagaagcggc gattgatggc cgacatttcg
     1921 gcgcgggcgg ccgacctgac tgttctgacg gccgaagacc cgcgcacgga gtcactcgac
     1981 gacattctgg cgatgatggc cggagcggca acggcagccg gcggcgtcga agacgaaacc
     2041 ttctggcgcg tgcccgaccg tggccgggcg atctatctgg cgctggagct ggcccaaccg
     2101 ggcgacgtgg tgctcatctg cggcaagggg catgagcagt cgatgtgctt cggcacagtg
     2161 gaatatccct gggacgaccg cacggcgacc gccgccgccc tcgatgcctg gcgcgagggg
     2221 cgcccgatgg gggatttggg gttgccgact tttggggagt gacgaccgcg actcctgtcg
     2281 cgcgacgaca gcaagacgac ggccggccga cgactgccgc aagagggcgc tcttcattcg
     2341 tcactcgcca ttataatcgg cacaaaccct ttccaggcgg gcgcaatggg ggtcggggtg
     2401 ggggtgacgg cggggccgac gtggaacgtg tggcggtggg tcgtcccgtt gaagtcggct
     2461 tcgaactgcc aggcgccggc cggcgcgtcc gcgggcaagt tgtaggccca ccaccagtat
     2521 gatgtggtgt aatggggctg ggcgatggcg tggctccatt gggtgaagat ggagccgtcg
     2581 ggctggatga tgcggaaggc ggtcggcttg ccggccacca gatcgcggta gtaggccagg
     2641 aatgtgaccc gatcgccggg ctgaaattgg gtggcttcat tgggcgtgtc ggggttgggg
     2701 cagctccccc aggtgggggc cttcgtccct gtcgccagcc ggttgatggc tgagtcgtag
     2761 tagttgcgct gctggatcca ccacgagccg ccgggcagct ggttgcactc gccgacatag
     2821 gggtcggtca gcgttccatt gtcgtataac tcgaagtgca ggtgcgggcc ggtggagttg
     2881 ccggagctgc cgaccgtgcc cagatactcc cccgcggcca ccgtcgcgcc tacgcctttg
     2941 ggcgtcagcg agtcgcgctt cagatggccg taccaggcca ccgagccgtc ggcgtggtac
     3001 acgtagacgg cgttccattt gttgccgttg agggtgcagc tgcggtcgaa gttgccgtcg
     3061 ctgcggccga tgatgacgcc gggagccgcg gccaccactt ccacatcccc ggcatccatc
     3121 ttgttccagg cgaacggcca caggaagagg tcggtgccct ggtggttgta gccggaaggg
     3181 ctgtcatagg tgcggccgcc gcaggcataa tcgagccgct ggttggggaa ggcggggttg
     3241 tggtccatga agccggagac ggcgtgatag ccgggatcgg cgaagcccgc gcggggccgc
     3301 agcggccagt ccagcgtcac ctgggccgag gcgcggggat cgggcgcggg cagcagccct
     3361 gtggcgctca ggcgcgccgt gttggcggcc agcatcgcct cgatctccgc ccgttcggcg
     3421 tcgctcatgc ggtcggccgt gggcagcaca tactcgccgc cgcccgacgc gaccgccgcc
     3481 gggcgctcgg ccggcccgcc cgcggccgcc ccggcgacga ggagcgttat gagggcgagg
     3541 aggaggtacg aagtacgaag tacgaagtat gaaaggcggg gctttttcgt acttcgtact
     3601 tcgtactttg gttgcgcggt cgtcttcttc ctcattcccc tattctagcc cgtcccgccg
     3661 cgttggggcg tgtccaatta agcaaataat cagatcaaca acccgtggag gggcgggaca
     3721 caaatcagtc acgtatccgc cgctgttaaa caaaccctgt tgcaacccgg ggacgggttg
     3781 caacagggat ccggcggccg cgtcaaccgg cttttgaata tttacaaatt aatcacgtat
     3841 acgctccggc ggattgaaat ccgctgctga tagacaaaac cggttgaaac cggttgccgg
     3901 cagaggcggc aaccgacttc agtcggtttg gcataacagc cgcgggattc atcccgcgct
     3961 tgattgatac gtgattattt tgttaacatg caacagccgg ttttgcctgc cggcggcggg
     4021 attgatcctg cctgctgcga acaaaagccc cctgctccct ttctcccttt ctccccttct
     4081 ccctttcccc ccaatcgtgc ccaatccatg aattaagcat ataattgcca tggtttaatc
     4141 accggcacat taccctgcga ccacccgctc ctatgattga tgaaaagacg gtctgggacg
     4201 acatctggcc ggttgtcgag caactcatct ccgccaccgt tgccgaagat tcccggactt
     4261 tgcgacaact cctgtacccc catggccagg cggccgacgc gctggagctg ttcggcccgg
     4321 tggtgttcga tatcctgctg aagacggtgc tgggccgcga ccggttgggg ctgacgcggg
     4381 cgattgaggg ggacggcggc gagaccgcct tcatcgaata cgcctggccc gacccggcga
     4441 cgcagggcag ctatacggcc gttgacgtgg tggctgtgcg gctggctcat ggcgatgccg
     4501 gctggcgcgt ggtggagatt aaccccgcgg cggccgacct gccgctgaac agcatgcggg
     4561 cgacgagcat tctggccggg atgcaggtga tgaacgagga cggcaagctg ccgtcggagc
     4621 cgtgggtgct gcccgtggcg ctctatgccg gggtgctgca gctgccgttg cagcccacgg
     4681 ccgcggccga tccggtcgag gcgctgctgt tgcccggcct gcaggcgcgg gggttcggct
     4741 ttttgccgca gatgctggcc cgtcgcctgt ggcgcgattt cgtcgcggcg gcaagcgcgg
     4801 gga
//
LOCUS       DAOMQG010000267         4793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7445_length_4793_cov_9.585690, whole genome shotgun sequence.
ACCESSION   DAOMQG010000267 DAOMQG010000000
VERSION     DAOMQG010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_4793_cov_9.585690"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLC06_12650"
     CDS             <1..498
                     /locus_tag="PLC06_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRO91342.1"
                     /translation="ERLELPTIALLHGHCLGLAFELALACDLRIAAEGTALGLPETRL
                     GIIPDVGGTTRLTRLVGPARAKELIFTGRRFDTALAVEWGIVNYTVPQEALLTKGDEL
                     AAEIAQAAPLAVGMAKRVIDGLVDIDRGLMLEGWAQSQLFGTEDFMEGAQAFMTRRPP
                     QFKGR"
     gene            572..982
                     /locus_tag="PLC06_12655"
     CDS             572..982
                     /locus_tag="PLC06_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRO91343.1"
                     /translation="MGIQFATDEWGKALVAAVNSSDAYAKAAEKWEGDFYFITTAGPG
                     TPKDHYLYLDLWHGKARDAYVVDDPSSKKVAFELRAPLDVWRKVLEKKIDPIRGIMSG
                     QLKLKGNMMTVLKAPKAATELVACASTVDTEWPQ"
     gene            1024..1878
                     /locus_tag="PLC06_12660"
     CDS             1024..1878
                     /locus_tag="PLC06_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRO91344.1"
                     /translation="MSSDDNRIKIGLALGGGVARGLAHIGVLSVLEDAGIPIDCIAGT
                     SMGAIIGSAYSAGLTIDELRTIAARTGWWQVSRPFFSAGGLVTFKRLEQWIEANVGEF
                     DVRDLAIPFAAVASDLASGERVVLSEGRLCTAVRASCSVPGFVPPVELDGRLLVDGGI
                     TDNIPADVARLLGADYVIGVDIFMPALRPHLGPLSQGIAAIETLVRHAGKGANECDCL
                     IVPCMKGRSYFKFSDYGQLIALGEEAALQQLPAIRESIARMESARKRSVTVRPVVAPD
                     LSPLHGMN"
     gene            1971..3491
                     /locus_tag="PLC06_12665"
     CDS             1971..3491
                     /locus_tag="PLC06_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91345.1"
                     /translation="MSFAPFAAIWVLLLFAAGVALIARRRPKIAIAATTISIILAAAL
                     WALFRPETIAPSLSLAGRQWAVGETAWHLTGIILLLCAAVVVRAAVDADSRHPRAMAI
                     ALGLPAATLPVVWAADDRTRLMGMALFAAAWAILFILISPTDSDSPRFTWRSGGLPLA
                     ALFLLWAAIAVPGGRAVFGVAAAALLIVAGAAADSRAKFGAAGTALHGFPVVVAAALL
                     GGILGTGSISLSGVMLGTVMGLTALLLGLAWVWDRPTSLASALALSLGGVVLIAAAWA
                     GQSALLAAARLAIFAPLLIATAWSGARQTLSSDVASDPNHESRRGLSPQLFALLVAYL
                     AVAGLPLMVGFGALAPIYESWSGATGWVLLLVTVALLSLWLAAIYQAGRATMRAATVD
                     PATWLRGLTLIPSVIGLISLDLTGLDAGVMTWAAIAIPVVAGVALGHFVPDMNAFGGL
                     LREAAALPQPLVNAAARVRGASRFAADALADALAILEGENGLIWLLGLLLLIIWIT"
     gene            3496..4197
                     /locus_tag="PLC06_12670"
     CDS             3496..4197
                     /locus_tag="PLC06_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91346.1"
                     /translation="MDVPTIFDILDRLAFLRGMPAVALLLAVAFIAVVAWDMRPAVVA
                     VLAHYLLAGFLFVDILDPRLAFVHALAGLFVTAILFITAWQTNWGRPPSGLTSLEATR
                     LGLSRTRQLGRLTVTDRNLLRLALATIVVLASLWLTRSPGLLQPVVPAELAYLEPAII
                     GLVGLGLVGLATSADPLPGGVGLLIFLTGFSLFYGFLDQSLAMTVALVIAQFVVALAV
                     AYLAQAHYLPADVLD"
     gene            4199..>4793
                     /locus_tag="PLC06_12675"
     CDS             4199..>4793
                     /locus_tag="PLC06_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91347.1"
                     /translation="METGFPLVMLVGPIVMAAVALAVRGHRRVTVIVGVVTVALLTML
                     LFLAAPGTGLFADNTVGFLGREAVLSPFVRSLFLFIYPAMGLIFLMAWTRPMGRALVP
                     VGLAVLSPLAAALMITPAGLGAVLLVAAAAVVVPTLHGGKFDAVAPVWRYFLLMAVAL
                     APILLVASGSMLPTTFPAWPAPLLATLILLGGFPFHLW"
BASE COUNT          684 a         1429 c         1556 g         1124 t
ORIGIN      
        1 gagcggctgg agttgccaac catcgccctg ctccatggcc attgtctggg gttagctttt
       61 gagctggcgc tggcctgcga cttgcgtatc gctgccgagg gtacggcgtt gggcctgccg
      121 gaaacgcgcc tggggattat ccccgacgtg ggcggtacga cacggctgac ccgactggtt
      181 ggccccgcgc gagccaagga gcttatcttc accggccgtc gttttgatac ggcgcttgcc
      241 gtggaatggg gcatcgtcaa ctacaccgtg cctcaggaag cactgctcac taagggcgac
      301 gaactggcgg ccgagatcgc ccaggctgcg ccgctggcgg tgggcatggc caagcgagtc
      361 atcgacgggc tggtcgatat cgaccgcggg ctgatgctgg aagggtgggc gcagagccaa
      421 ctcttcggca ccgaggattt catggaaggt gcccaggcct ttatgacccg ccgcccgccg
      481 caatttaagg ggcgctaatt ggcaagcccg cgcgggttat gctatcttac cgattatcta
      541 tagaagcaat attgtatacg gaggcgaaaa gatggggatt cagtttgcga ccgacgagtg
      601 ggggaaggca ttggtggcgg ccgtgaacag cagcgacgcc tatgccaagg ccgcggaaaa
      661 atgggaaggt gatttttatt tcatcaccac tgccgggccg ggcacgccca aagaccatta
      721 tctttatctc gacttgtggc acggcaaggc tcgcgacgct tatgtggtcg acgacccgtc
      781 ttccaaaaag gttgccttcg aattgcgcgc gccgctggat gtatggcgca aggtgctgga
      841 gaaaaagatc gatccgatcc gcggcatcat gtccggccaa cttaagttga agggcaacat
      901 gatgaccgtg ctcaaggccc ccaaggcggc gacggagctt gtggcttgcg cctcgacagt
      961 tgacacggag tggccgcagt aacgcaaccg tttttcggtt gatggcggag cagggagcgg
     1021 ttcatgagca gtgacgacaa ccgcatcaaa ataggtctgg cgctgggggg tggcgtggcg
     1081 cggggattgg cccacatcgg cgtcttgtcc gtcctggaag atgcgggcat tcccattgac
     1141 tgcatcgccg gcaccagcat gggcgcgatt atcggctcgg cttatagcgc cggtttgacc
     1201 atcgatgagc ttcgtacaat tgccgctcgc accggttggt ggcaagtatc acgccccttc
     1261 ttttcggccg gcggcctcgt gaccttcaag cgattggagc agtggatcga ggccaatgtc
     1321 ggcgagttcg acgtgcggga tttggccatt ccctttgccg ccgtggccag cgacctggct
     1381 tccggcgagc gcgtggtctt gtcggagggg cgcttgtgca cggccgtgcg cgcgagttgt
     1441 tccgtccccg ggttcgtgcc gccggtagag ctggatggtc gcctccttgt cgacggcggc
     1501 atcaccgaca acatccccgc tgatgttgcc cgcctgctgg gggctgacta tgtgatcggg
     1561 gtcgatatct tcatgcccgc gttacggccg catttggggc cgcttagtca gggcattgcc
     1621 gctattgaaa cgctcgtgcg ccacgccggg aaaggggcca atgagtgcga ttgcctgatc
     1681 gtaccttgca tgaaagggcg ctcctacttc aagttctccg attacggcca gctgatcgcg
     1741 cttggcgaag aagcggcctt gcaacaacta ccggccattc gcgagtcgat agcccgcatg
     1801 gagtccgccc gcaagcgttc agtgactgtt cggccggtcg tcgcccccga tctcagcccg
     1861 cttcacggca tgaactgact cggccacgcg gccgaaaaac ccacctgatg cttatactat
     1921 cctgaagcct cattgcttga tagacgcttc gggtctagaa tcgatctttg atgtctttcg
     1981 caccctttgc cgcgatttgg gtacttctgc tttttgctgc gggcgtcgcg cttattgcaa
     2041 gacggcgacc gaagatagcg atcgcggcta ccaccatatc aattatcctg gctgccgctc
     2101 tgtgggcctt gttccgcccc gaaacaatcg cgccatcgct gagtctcgcc gggcggcaat
     2161 gggcggtagg cgagactgcc tggcacctga ccgggatcat cctcttgttg tgcgcggcgg
     2221 tagtcgtccg ggcggccgtc gatgccgatt cccgccaccc gcgcgcgatg gctattgctc
     2281 tgggcctgcc ggccgccacc ttgccggtgg tgtgggcggc cgacgatcgg acccggctga
     2341 tgggcatggc tctgtttgcc gcggcctggg cgatcctgtt catcctcata agcccgacag
     2401 attccgattc accacgcttc acctggcgca gcggtggatt accgttggcc gcactcttcc
     2461 tgttgtgggc cgccattgcg gtgcccggcg ggcgggctgt gttcggtgtg gccgcggccg
     2521 cgcttctgat cgtcgccggc gcagcggccg attcccgtgc gaagttcggg gcagccggaa
     2581 ctgccctgca cggattccct gtcgtcgttg cggccgctct ccttggcggg attctcggca
     2641 caggctcgat atctctgtca ggcgtcatgc ttgggacggt catggggttg acggctctgc
     2701 tgttgggttt ggcctgggtg tgggatcggc cgacgtcgtt ggcttcggcg ctcgccctgt
     2761 cattgggcgg cgtggtattg attgccgccg cctgggccgg acaatcggcg ttgcttgcgg
     2821 cggcccgatt ggcgatattt gcccccctgc tcatcgcgac ggcatggagt ggggcacgtc
     2881 agacgctatc gtctgacgtg gcttccgacc ccaatcatga atcgcgccgt ggcttatcgc
     2941 cgcaattgtt cgctctgctg gtggcttatc tggccgtggc cggattgcca ttgatggtgg
     3001 gattcggcgc tcttgcgccg atctatgaat catggagcgg tgcgaccggt tgggtattat
     3061 tgctggttac cgttgcgttg ttgagcctgt ggctggccgc gatctatcaa gcgggacgag
     3121 caacgatgcg cgccgcgacg gtcgatcctg ccacgtggct tcgcggattg accctcatac
     3181 cgtcggtcat tggtctcatc tcgcttgatt tgaccgggct tgacgccggc gtgatgacgt
     3241 gggcggccat cgctattcct gtagtggccg gggttgcgct aggccatttt gtgccggata
     3301 tgaatgcatt tggcggcttg ctgcgcgagg cggccgcact cccgcaaccg ctggtcaacg
     3361 ccgcggcgcg agtacggggg gcgagccggt ttgccgcaga cgcgctggcc gatgccctcg
     3421 ccatactgga aggagaaaac ggcctgatct ggctgttggg gcttttactc ctgatcatct
     3481 ggatcaccta aatttatgga cgtgccaacg atcttcgaca ttctcgaccg tctggctttc
     3541 ctgcgcggca tgccggccgt cgccctgttg ctggcagtgg ccttcattgc cgtcgtggct
     3601 tgggatatgc ggccggccgt cgtcgctgtt ctcgctcatt atctgctggc cgggtttctg
     3661 ttcgtggata tcctcgatcc ccgactggcc tttgtccatg ccctggccgg gttatttgtg
     3721 accgcgattc tgtttatcac cgcctggcaa acaaattggg gacggccgcc gtcgggcctg
     3781 acatcgctgg aggcgacgcg actcggcctc tcccggacgc gccaattggg ccgcctgacc
     3841 gtcaccgatc gcaacctgct tcgattggct ctggcgacca tagtcgttct ggcctcctta
     3901 tggctgacgc gatcgcccgg cctgttgcag ccggtcgtcc ctgccgagtt ggcctatctg
     3961 gaaccggcga tcatagggct ggttgggctt gggctggtcg gtctggcaac ctcggccgat
     4021 ccgcttcccg ggggtgttgg cctgctcatt ttcctgaccg gcttcagtct gttctatggc
     4081 ttcctcgatc aatctctggc gatgaccgtg gctctggtta tcgctcaatt tgtcgttgcg
     4141 ctggccgtgg cctatttggc tcaggcccac tatctgccgg ccgatgttct ggactgagat
     4201 ggagaccgga tttcccctcg tgatgctggt tgggccgatc gtgatggcgg ccgtggcgct
     4261 ggccgttcgt ggtcatcgcc gcgtcaccgt gatcgtcggt gttgtcaccg tcgccctgtt
     4321 gaccatgctg ctgtttctgg ctgcgccggg gacagggctg tttgccgata atacggtcgg
     4381 ctttctgggc cgcgaggcgg tgctatcgcc tttcgtccgc tcactgtttc tgtttatcta
     4441 tccggcgatg gggctgatat ttctcatggc ctggacgcgg ccgatggggc gggcgctcgt
     4501 gccggttggc ttggctgtgt tgtcaccctt ggcggccgcg ttgatgatta cgcccgccgg
     4561 tcttggcgcg gtgctgctgg ttgccgcagc ggccgtcgtt gtcccgactc tccacggcgg
     4621 caaatttgac gccgttgcgc ctgtgtggcg ttattttctg ctgatggccg tggcgctggc
     4681 gcccatcttg ttggtcgctt ccgggtcgat gctccccacg acttttcccg cctggccggc
     4741 tccgctgctg gcgacgctca tccttctggg cggcttcccg ttccaccttt ggg
//
LOCUS       DAOMQG010000268         4789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7451_length_4789_cov_8.950993, whole genome shotgun sequence.
ACCESSION   DAOMQG010000268 DAOMQG010000000
VERSION     DAOMQG010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_4789_cov_8.950993"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLC06_12680"
     CDS             <1..809
                     /locus_tag="PLC06_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRO91348.1"
                     /translation="DSLTLAQQHLYTFQACPRRFFLRFLARIPWPEAPLGPEQEQAYE
                     RGRRFHRWIERRFLGLPVADESDHDPVLNAWWNIYLRHAPPLPEGRRFVETSLTVPIG
                     QGGKHRLTGRFDLLVVGDNPPAASLFDWKTGDPRPIERLRRAWQTRVYLFLAAEGGAA
                     LVPDNPAAFEPERISLTYWYVEDPTRPRHIPYDAAAHRRNRTELEAIVAEIDRQLVEN
                     DWPLTDDWLECRHCPYQAYCGRQAAGHEASDDTPDEDEEPGDLWLEPQWG"
     gene            819..2018
                     /locus_tag="PLC06_12685"
     CDS             819..2018
                     /locus_tag="PLC06_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRO91349.1"
                     /translation="MDSEALAYLLGPHGRALLDELTAESLSPDTRLATATRLRRHLEP
                     GIAAAVMEMALLRQRAARKFSRADCMFFTRDGLEQATAEPVAIHRAGRYAAAGIASVA
                     DLGCGIGGDALALAGTGAQVIAVDKEWNHIMLARENAAVYDVGEYVHPVLADLTELPA
                     LAVGAFFFDPARRTPTGPQLAPGRRLHSVEEYRPPLSLINAWRPQVSNGAVKVSPGID
                     YAELPADVEAEFVSLDGEVREAALWFGELRTGIARRATLLPGGHTLTDRDPDESPVLP
                     PRRYLYEPDGAVIRAHLVGQLAHLISASRIDPQIAYLTTDTPQPTPFAKGYAIDDYFP
                     FQLKRLRSYLRERDVGNVTIKKRGSPLDVDELRQALRLRGHHNRTLFLTQIMGQPTVL
                     IGQPLDA"
     gene            complement(2083..2823)
                     /locus_tag="PLC06_12690"
     CDS             complement(2083..2823)
                     /locus_tag="PLC06_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91350.1"
                     /translation="MIDFATIDDDEDERVNYFKLDDNVIYIMGQGMTSRGRFIIPYDG
                     VLSYEANDSIGMFIGLCNKKVTPTPTMTLTPTATLEPTFTPTPTLTPTMVLTPTATVT
                     GTLTATPVFTPTATSTPIPFTPTAEPEIITATPSPSPTPTREPRLPACLRINFEVSGQ
                     AARRGLYVVQEVGGRFLTAWEADDGWRDSGWIYDIDITFDAVYVDVLYYHGPGAEPVK
                     MVIWNPAPGTTNGWLARGMCHALEVGWP"
     gene            3555..4484
                     /locus_tag="PLC06_12695"
     CDS             3555..4484
                     /locus_tag="PLC06_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRO91351.1"
                     /translation="MRYIITGGSGVIGTALTRSLAADGHEIIILSRDPSRHWLPDGAR
                     GVKWDARTAGGWGHLANGAFAVINLAGETIGGSSAIPLPGTWSPERKERIKNSRLWAG
                     EAIVAAIEGATDKPKVVYQMSGIDYYPPGDTMMTEESKRGRGFLTEVVADFWEPSTEP
                     VEAMDVRRVVGRTAPLLNLKTGPLPASLLQFRLYAGGRLGNGKQWFSWIHTKDAVRAI
                     RFLTETPAAKGIYNLAAPNPVTNGEFTHILGKVMNRPTFLPVPEFALKLLLGEVSALV
                     LQGRPVSAEKLEGLGFTFNYSTLEAALRQILNK"
     gene            4637..>4789
                     /locus_tag="PLC06_12700"
     CDS             4637..>4789
                     /locus_tag="PLC06_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRO91352.1"
                     /translation="MSRNPDINLTVNDRSTRGYLALPEGDGPWPGVVVIQEWWGLNDN
                     IRDTADR"
BASE COUNT          986 a         1541 c         1355 g          907 t
ORIGIN      
        1 ctgacagtct gacgctcgcc cagcaacacc tctacacctt ccaggcctgc ccgcgccgct
       61 tttttctgcg cttcctggcg cgcatcccct ggccggaagc gccgcttggc ccggaacagg
      121 agcaagctta cgagcgcggc cgtcggttcc accggtggat cgagcgccgc ttccttggcc
      181 tgcccgtggc cgatgaatcc gaccacgacc ccgtgctgaa cgcatggtgg aacatctatc
      241 tgcgccacgc gccgccactg cccgaaggtc gccggttcgt cgaaacatcc ctgaccgtgc
      301 ccattgggca aggcggcaag caccgcctca ccggccgctt cgacctgctg gtggtcggag
      361 acaacccgcc cgcggcgagt ctgttcgact ggaaaacggg cgatccccgc cccatcgagc
      421 ggttgcggcg cgcctggcaa acgcgagtct acctgtttct ggcggccgaa ggcggcgcag
      481 cattggtccc cgacaatccg gccgcgtttg aacccgagcg aatctcgcta acttactggt
      541 acgtcgaaga tcccacccgc ccgcgccaca ttccctacga cgcggccgcc catcgtcgta
      601 atcgcaccga gctggaggca atcgtggccg agattgatcg gcaattggtc gaaaacgact
      661 ggcccctcac cgatgactgg ttagaatgtc gtcattgccc ctatcaggcc tactgcggcc
      721 ggcaggccgc cggacatgaa gcctcggatg atacgccgga cgaagacgaa gagccgggcg
      781 acctgtggct ggaaccccaa tgggggtaat cgccggtaat ggattccgaa gcattagcgt
      841 acctgcttgg cccacacggg cgagcgctcc tggatgaact gacggccgaa tcattgtcgc
      901 ccgacacccg gctggccaca gccacacggc tgcgccggca cctggaaccg ggcatagccg
      961 ccgcggtgat ggagatggcc ttgctgcggc aacgcgccgc ccgaaagttc agtcgggcag
     1021 attgcatgtt cttcacccgc gacggcctgg agcaagccac ggccgaaccg gtggccatcc
     1081 accgcgccgg acgctacgcc gctgccggca tcgcttccgt cgccgatctg ggttgcggca
     1141 ttggcggcga cgccctggct ctggccggca ccggcgcgca ggtcatcgcc gtcgacaagg
     1201 aatggaacca catcatgctg gcgcgagaaa acgccgccgt ctatgacgtg ggcgaatacg
     1261 ttcatcccgt tctggccgac ctgaccgaac tgccggcgct tgcggtgggc gcgttcttct
     1321 ttgacccggc gcggcgcacc cccaccgggc cgcagctcgc cccaggccgt cgtctgcatt
     1381 ccgtcgagga atatcggcca cccctgagtc tcatcaatgc ctggcggccg caagtgtcga
     1441 atggcgcggt caaggtcagt cccggcatcg actacgccga actgccggcc gatgtcgagg
     1501 ccgagttcgt ctcgctcgac ggcgaagtgc gcgaggcagc gttgtggttt ggcgagttac
     1561 gcaccggaat cgcccgtcgc gccacgctgt tacccggcgg tcacacgctg accgaccgcg
     1621 accccgacga atcgcccgtg ctgcccccgc gccgctatct ctatgagcct gacggagcag
     1681 tcatccgcgc ccaccttgtc ggtcagctcg cccatctgat cagcgcttcc cggattgacc
     1741 cccagatcgc ctacctgacg accgacaccc cgcagccgac tcccttcgcc aaagggtatg
     1801 caatcgacga ctatttcccc tttcagttga agcgcctgcg gagctatctg cgagagagag
     1861 atgtgggtaa cgtgacgatc aagaagcgcg gctcgccact ggatgtggac gaactgagac
     1921 aagcgttacg cttgcgcggc catcataatc gtaccctctt cctgacgcag atcatgggac
     1981 agcccaccgt actcattgga caaccgctgg atgcatgaaa aaaggcggat gggttattcc
     2041 atccgccttt cgccttatta ttgagcgctc cctcatgacg ctctaaggcc agccgacctc
     2101 cagcgcgtgg cacatgccgc gcgccagcca accgttggtt gtgcccggcg caggattcca
     2161 aatcaccatc ttcaccggtt cggcgccggg gccgtgataa tacaatacat ccacatagac
     2221 ggcgtcgaac gtgatatcta tatcataaat ccagccgcta tcccgccaac catcgtccgc
     2281 ctcccaggca gtcaggaatc gcccacccac ttcctgaacc acgtaaagcc cgcgccgtgc
     2341 cgcctggccg ctaacctcaa agttgatgcg caagcaagcc ggcaagcgtg gctcgcgcgt
     2401 cggggtcggg ctgggcgatg gcgttgccgt gattatctcg ggttcggccg ttggcgtgaa
     2461 tggaattggc gtcgaagtcg ccgtcggcgt gaacaccggg gtagccgtga gcgtgccggt
     2521 aacggtagct gtcggcgtca acaccatggt cggcgtcaat gtcggggtag gcgtgaaagt
     2581 tggctccaac gtggccgttg gcgtcagcgt catggtcggc gtcggcgtca cctttttgtt
     2641 gcagaggccg atgaacatgc cgatgctgtc gttggcttca taactcaaca ccccgtcata
     2701 gggaataata aatcggccgc gcgacgtcat cccctgcccc atgatataga tcacattgtc
     2761 atcgagtttg aaatagttga cgcgttcgtc ctcgtcgtcg tcgatggtgg caaagtcgat
     2821 cacacaattt ttttccaccg ggccaacaat cctggtgaca ttatgatccg gcgccggagc
     2881 tccggtactg ccatacaccg accacacacg ctcgccatca cgcgcggtat attctggtcc
     2941 aggcatcacc ataaatccaa agggatggcg cgttttaacg ataaaattgt acccctcgcc
     3001 caggctataa cttccacctt cccccaggta ggtcagcgcc ggcacatctc cgtccgcctg
     3061 tacctgccaa gcctgaatca atgtcaaaag cgccaggcca acccccaggc aaaggaaaaa
     3121 tattgtggta attcttaatg cgtactgctt cattattact acctcaaaac cctatccact
     3181 acgactcacc ttgagtgtat gtacctagag gcaggcaaaa tgggtagtac cttacaggaa
     3241 aactgtaaag cagtttacac ttacgtgaac ccctcgttag aatgcttcac gcctgtaatc
     3301 tttgtaacac tttttgtaac atttcatgat gacataacgt tcctcaagcc atgtacttcg
     3361 cgaggtgaac ccattcggcc gccctgcccc acccgtacta ccattaatct atatgccttc
     3421 gcccatcgcc ctcgacaaat ccggggcaaa atcgtaaggt ataggcagga tttccgcgag
     3481 gagccgaatc acgtgcgccg agagccaaag acaatcagac cggctcatag cgcgctaaac
     3541 gggagaattg cttcatgcgc tacatcatta ccggcggttc gggagtcatc ggtactgcgc
     3601 tcaccaggag tctggccgcc gacggccatg aaatcatcat cctgagccgc gatccgtccc
     3661 gacattggct acccgacggg gcacgcggcg tcaagtggga cgcccgaacg gccggcggct
     3721 ggggccacct ggccaatggc gcgttcgctg taatcaacct ggccggcgaa acgatcggcg
     3781 gcagcagcgc gatcccgctt ccgggaacct ggtcacccga acgcaaagag cgcatcaaga
     3841 acagcaggct gtgggccggg gaagcgattg tcgccgccat cgaaggggcg accgacaagc
     3901 ccaaagtcgt ctatcaaatg tcgggcatcg actactatcc gccgggcgac acgatgatga
     3961 ccgaggagag caagcgcggc cgaggattcc tgaccgaggt agtcgccgat ttttgggaac
     4021 catccacgga acctgtcgaa gccatggacg tgcggcgcgt ggtgggccgt accgcgccac
     4081 tgctcaacct gaagacaggt cctcttccag cctcgctgct tcaattcagg ctctacgccg
     4141 gtggccggct cggcaacggc aaacaatggt tttcatggat ccacaccaag gacgccgttc
     4201 gggcgatccg cttcctgacg gaaacaccgg ccgcgaaggg tatctataac ctcgctgccc
     4261 ccaatccggt gaccaatggg gagttcaccc acatcctggg caaggtgatg aatcggccga
     4321 ccttcttgcc ggtgccggag ttcgcgctca agctcttgct gggagaagtg tcagccctgg
     4381 tgctgcaggg gcgcccggtc agcgcggaaa aactggaggg attgggtttc accttcaatt
     4441 actcgactct ggaagctgct ctgcgccaaa ttctgaacaa ataatcgacg acgcaataga
     4501 ccgtcgccga tcgcgtcata catatagtca ggcccaggac gaatataggt taatcatcac
     4561 gcttgcgctt cgggctaaca atggctacga tgacgatcgt tacttgataa ccaaacctga
     4621 ttggaaggaa accgacatgt ctcgcaaccc cgatatcaat ctaacggtca acgatcgctc
     4681 tacccgcggc tatctggcac tgccggaagg cgatggcccc tggcccgggg tagttgtcat
     4741 ccaggaatgg tgggggctga acgataacat tcgcgacacg gccgaccgg
//
LOCUS       DAOMQG010000269         4779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7463_length_4779_cov_9.618755, whole genome shotgun sequence.
ACCESSION   DAOMQG010000269 DAOMQG010000000
VERSION     DAOMQG010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_4779_cov_9.618755"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLC06_12705"
     CDS             <1..951
                     /locus_tag="PLC06_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91353.1"
                     /translation="LQLTIVPIIYTDTVTGKTYSTPGHDPISSWLRSAFPISDVIVDI
                     HAPLLFTGDLRRGSDWGLLLQNITALWGNEVGSGSPHIYYGLIPNIASDGSSWFYGGL
                     SGLGWVGQRVSIGIDLGLDTGPSAAHEIGHNLGRRHAPCGNPSGVDPNFPYANASIGV
                     YGVDTTAETLLTPDQTHDLMSYCGPEWVSDYTYEALFQDQSLRGNQMGVTGDGLFVTT
                     LIDGNDNASFLATGETGIDIASGSVQGIQVQVVDRKGAILGTYPAEMYSAEEEGVSVT
                     MLGAFVPGLGNGQSIKKFRFMRGDVVIAEQLVREVVVPQQ"
     gene            complement(958..1569)
                     /locus_tag="PLC06_12710"
     CDS             complement(958..1569)
                     /locus_tag="PLC06_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRO91354.1"
                     /translation="MNNDIKAIIFDVGGVLLRTADQSGRQKWETRLGLLPGGAEAIVL
                     NSEMGYRAQRGEISTETLWAWVGARLKLGNDLTQFRQDFWQGDFVDQSLVALVRKLRQ
                     RYQLAIISNAMDSLTETLTEYALLDEFDLIVGSAYEGVMKPDPVIYRRTLHRLGVSPQ
                     ESVFIDDSSANIAGAKALGINAILFTPTIDLPYELARIGVEIP"
     gene            complement(1562..>2509)
                     /locus_tag="PLC06_12715"
     CDS             complement(1562..>2509)
                     /locus_tag="PLC06_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91355.1"
                     /translation="QHVIVAPHISSILATQQHDSSEQVARQILSALSHRRTSETLDLE
                     VVPIEQIVPHEHIDLKRVSRLVERLEDDGRLVNPPVTTYWKGRYVILDGATRYSALRQ
                     LGYANGIVQVVDKDQAGFQLHTWYHAISEEQQIGDVPSFDKLVDVLQKVPGLNLKPIN
                     ADEAQGALLRPNSLCYFIDREGRMLLAEAGPGASKLAVMNGIVDTYNAWGVVERTLLT
                     DMDRLLAQFPRLVAVAVYPQFNPEEVFDAAADGNLLPAGLTRFVIPGRILRLNADLAR
                     LKRDEPLAEKRAWFNEFLSGKLSRSRFRVYQEPVVLLDE"
     assembly_gap    2510..2609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2610..2968)
                     /locus_tag="PLC06_12720"
     CDS             complement(<2610..2968)
                     /locus_tag="PLC06_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91356.1"
                     /translation="MLKGVGQVDVDYEADEEALIAKIGNYHALIANPNQRINSHIIKY
                     GYNLKAIGSLDGNLDKIDVGQARALGIEVCYAPDSRSVTIAEHTIGRLLSAADKFAGG
                     SLAGKTLGIIGFGLVGR"
     gene            complement(3116..4042)
                     /locus_tag="PLC06_12725"
                     /pseudo
     CDS             complement(3116..4042)
                     /locus_tag="PLC06_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019846041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            complement(4073..4537)
                     /locus_tag="PLC06_12730"
     CDS             complement(4073..4537)
                     /locus_tag="PLC06_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91357.1"
                     /translation="MSIVKDARQIRYLQHDEVLELHCRRKERMNWLSSVETMHNVRRL
                     ANFSPDDEAGYLQHELAHLSGIIVEFSDGARGWVSFQATTLQLKRICVEVLRGDPVRT
                     TAGLLELLHRLHSSQDAVVENIPDDAAWPGYKEAGYFEVFRRIEMVRESVTE"
BASE COUNT         1014 a         1273 c         1302 g         1090 t
ORIGIN      
        1 ttgcagctga ccattgtgcc gatcatctat acggatacgg tgacgggaaa aacatattcg
       61 acgccgggcc atgatcccat cagcagctgg ctccgatccg cattcccgat cagcgatgtg
      121 atcgttgata tccatgctcc tttgctgttt acgggtgatt tgcgtcgtgg cagtgattgg
      181 gggcttttgc tgcaaaacat aactgcactc tggggcaatg aagtgggctc cggttcgcct
      241 cacatctatt atggcctcat tcccaatatc gcttcagatg ggagtagttg gttctatggc
      301 ggtttgagtg gccttggatg ggttgggcaa agggtatcca tcggcatcga ccttggcctc
      361 gacaccggtc cgtcggccgc ccatgaaatt ggccataacc tgggccgtcg ccatgcgcca
      421 tgcggcaacc catccggtgt cgacccgaat ttcccctacg ctaacgcatc gatcggtgtg
      481 tatggggtcg atacgacagc cgaaacgctg ctaactcccg accagaccca tgacttgatg
      541 agctattgcg gccctgaatg ggtgtcggat tacacgtatg aggcattatt tcaggatcaa
      601 tcgctccgcg gcaaccaaat gggtgtcacc ggagacgggc tatttgtaac aacccttatc
      661 gatgggaatg ataacgcttc attcctggcg acgggtgaaa ccggcatcga tatagcatcc
      721 ggttctgtgc aaggaattca ggtgcaggtg gttgatcgta aaggggctat cttgggaacc
      781 tatccagctg aaatgtattc ggccgaggaa gagggcgtat cggtgacgat gctcggtgct
      841 ttcgtgcctg gcctgggaaa cgggcagagc atcaagaagt tccgatttat gcgtggtgat
      901 gtggtgatcg cggaacagtt ggttcgtgag gtagttgtcc cgcaacagta aatcgcatta
      961 tgggatttcg acgccgatcc tggccaattc atagggcagg tcaatggtgg gcgtgaacag
     1021 gattgcgttg atgcccagcg ctttagctcc ggcgatgttg gctgatgaat catcgatgaa
     1081 gacgctctcc tgaggggata cccccaatct atgcaaggtt cgtctataga tgacgggatc
     1141 gggcttcata acgccctcat aggcggagcc cacaatcaga tcgaactcat cgagcaacgc
     1201 atattcggtt aatgtctccg tcagcgaatc catcgcgttg ctgatgattg ccagttgata
     1261 gcgttgacga agtttgcgga ccagcgctac cagcgattga tccacgaaat ccccttgcca
     1321 aaaatcttgt cgaaattggg tcaggtcatt gcccagcttc aagcgcgccc caacccatgc
     1381 ccacagcgtc tcggtagaaa tctcccctcg ctgggcccgg tatcccatct cgctattgag
     1441 gacaatggct tcagctccgc caggcagcag gcctaatcgc gtttcccact tttgccggcc
     1501 ggattgatct gccgtgcgta acaatacgcc acccacgtcg aagatgatgg ccttaatgtc
     1561 gttattcatc gagcaagact accggttcct ggtacacacg gaaccggctg cgtgacaatt
     1621 tgcctgaaag gaactcgttg aaccacgcgc gtttttcggc cagtggctcg tctcgtttca
     1681 accgggcgag atcggcattg aggcgcaaaa tgcgaccggg gatgacgaat cgggtcaagc
     1741 ctgccggcag cagattgccg tctgctgccg cgtcgaatac ctcttccgga ttgaattgcg
     1801 ggtaaacggc gactgcgacc agccgtggga actgggccaa taatcggtcc atgtcggtca
     1861 gcaacgtgcg ctctacgaca ccccaggcat tgtaggtatc aacaataccg ttcatcacgg
     1921 ccagcttgga cgcaccgggg ccggcttcgg ccaacaacat gcggccttct cgatcaataa
     1981 agtagcaaag agaattcggc cgcaaaagcg cgccctgggc ttcatcggca ttgatgggtt
     2041 taaggtttag ccccggcact ttttgtagca catcgaccaa tttatcgaag gatggaacat
     2101 cgccgatctg ctgttcttcg ctgatagcat gataccaggt atgaagctgg aacccggctt
     2161 gatccttatc caccacctgg acgatgccgt tggcatagcc aagttggcgc agggcgctat
     2221 agcgagtcgc gccgtcaaga atcacatatc gccccttcca gtaagtagtc acgggagggt
     2281 tgaccaggcg tccatcgtct tcgaggcgct caaccagtcg tgatacgcgc ttcagatcga
     2341 tgtgttcgtg cggaacgatc tgttcgatcg gtacaacttc aagatcgagc gtttcgcttg
     2401 tgcggcgatg ggagagtgcc gacaggatct ggcgcgctac ttgttccgag ctatcgtgtt
     2461 gctgggtggc cagaatggag gaaatatgcg gcgccacgat gacgtgctgn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gtcggccgac cagaccgaaa ccgataatgc
     2641 ccagtgtctt gccggccaaa cttccgcccg cgaatttgtc ggccgcagag aggagtcgtc
     2701 cgatggtatg ttcggcaatc gtgactgagc ggctatccgg tgcatagcat acttcaatac
     2761 ccaaagcccg cgcctggccg acgtcgatct tgtcgaggtt cccatcaagg gagccaatgg
     2821 ctttgaggtt atagccatac ttaataatgt gggagttaat acgttggttc ggattagcga
     2881 tcaacgcatg ataattgcca attttggcga tgagcgcttc ttcatcggcc tcataatcaa
     2941 catcaacctg tcccacacct ttcagcattt gcaatgcagt cggggcgatg ggggcgcaaa
     3001 ccaggattcg ggagggtttg cgttggcggt tattcttttc ttccagatcg atggggggtt
     3061 tcgtggtaat cggttggggc gccgtagttt tatcccttgt gggaaagcca taagccgcca
     3121 tattgatatt gccgccgctt atgagaatgc ctacccgtga ctcaggcggt aacggataag
     3181 ttccgctgag gattggcgca agagcgacgg ccgaactagg ctcgactacg atttttaggc
     3241 gttcccaaat gaattcaaga gcgttaagga tatccgcctc gctgacgctg atcatgtcac
     3301 taacgtaacg gcgcatgatc tcgaggttac gttggccgat ggctcgggtg cgcaggccgt
     3361 cggccacggt ttccggtacg ctttcgagct tgatgacgac gccttcacgc caggagcgac
     3421 cggcatcgtc gcccagtttc ggctcgaccc cgatgatttg gcagtcgggt gcccgcaatt
     3481 gggcggccag cgcactgccg ctgatgagac ctccgccacc tacgggcaca aacagataat
     3541 caagttggcc cacttcgtcg aatagttcca gagcggccgt accttgcccc gcgatgatat
     3601 ggtcattatc gaaagggtgg atcagcgtat aaccatgatc ggcgatcagc ttcgccgtca
     3661 cctcatcacg ctccatggca ttacagacga taacctcggc tccgtaccct tcagttgcag
     3721 cccgcttgtt ggcgggcgcg tcttccggca tcaccaccac cgcgcgcacg ccaagcaatt
     3781 gagcggccaa tgctacccct tgcgcgtggt tgccgctgga atgggtgata accccggccg
     3841 atttttcatc ggcgctcaac cggctaattg cgttgtatgc gccacggaac ttgaacgcgc
     3901 cgacgcgctg gaaattttca catttgaaat agatctgaca tttcatcaga tcattcaacg
     3961 tttgggaggt gagaacgggt gtgcggtggg ccttccccgc gatttgtctt ttcgcctcaa
     4021 gtacagattc aggggagatc atttgatcct cgtagttggg aatccgagat atttattcag
     4081 tgactgattc gcgcaccatc tcgattcgtc gaaatacctc aaagtatccg gcctctttgt
     4141 agcccggcca cgctgcgtca tccgggatgt tttcgacaac agcatcctgg gaactatgga
     4201 ggcgatggag caactccagc aggccggctg tcgtgcgaac cggatctcct cggagcactt
     4261 cgacacaaat gcgtttaagt tgcagggttg ttgcctgaaa agacacccaa cctcgtgccc
     4321 cgtctgaaaa ttcaacgatg atgcctgaga gatgggccaa ttcatgttga agatagcccg
     4381 cctcgtcatc cggcgaaaaa ttggccagcc gccgcacgtt gtgcatcgtc tcaacactgc
     4441 tgagccaatt cattcgctcc tttcgccggc aatgaagttc cagaacctca tcgtgttgta
     4501 aatagcgtat ctggcgcgcg tccttgacaa tagacatatt gcgagccgtt cgtggaggac
     4561 gtcgggcaac gattagctcg cgagtctcgc gaaattgaaa cttgtggaaa agctcatgag
     4621 cgggtccatt cccatgaatg acctcgagcc agacgtccgg gagtccgcag ccatgggctt
     4681 cggtcagaag cgcatccaga attgcaccac caacacctcg acgtctgccg tcggggagga
     4741 caccaagccg cgtaatccac cccatgcctt tccgtttgc
//
LOCUS       DAOMQG010000270         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7476_length_4774_cov_9.643145, whole genome shotgun sequence.
ACCESSION   DAOMQG010000270 DAOMQG010000000
VERSION     DAOMQG010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7476_length_4774_cov_9.643145"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLC06_12735"
     CDS             <1..492
                     /locus_tag="PLC06_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91358.1"
                     /translation="QDGFAELEAHLRACPDCNREWSGLMAFELLFRQTPILMPALDFT
                     ERTLARLPNRRARRVAMGTAYAVLLLSGIAPLLIGLFLVARYWAVLSSPALLGGVWAS
                     VAGVGRAILTVIEALLAGASHFVIEQPILIGWFIILAGLVFLWGGVFQRLLMQPGGAG
                     LRN"
     gene            543..1712
                     /locus_tag="PLC06_12740"
     CDS             543..1712
                     /locus_tag="PLC06_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91359.1"
                     /translation="MKKSVLIPILLAALLILALAPATFAAPPADTIIESGEVVNNDVV
                     VFDGDLEIQSGATVNGDVAVFNGDAVIDGQVNGSLTLFNGDLQAGAGGHITGECVLLN
                     GQAAGDGFADRNSSSCTAIQSLNVAPLAELGPWLRDLQAIPGVPEVPAVPEVPVVPAV
                     PEVPAVPAVPGIQAPHRGRGGFGAELFGVFASSLLFGFLALMTAAIMPNQLRQITTTA
                     RDRSMVSGMAGALTAVAVPSLIVLLIPISIILTFVCIGLLGFPIMFLLGLALVVGSFL
                     GWIAVGTWLGIRIFGRGKSGDRIVRSAALGTALLTFITGVLGLLTFGVAGGILVFIAL
                     SIGLGAVALTQFGMKPYPRRSRSNAPGSGTPGNDSIDPDKYDKVLNTLPPEEAAA"
     gene            complement(1818..2465)
                     /locus_tag="PLC06_12745"
     CDS             complement(1818..2465)
                     /locus_tag="PLC06_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRO91360.1"
                     /translation="MTIPPPPPWREAARAAMYEATQGELLTRYGTTEVSFNYRWEHVT
                     AVVALALRLAELTGADAEIVEAAAWLHDVCKRTDGNRHPIAGAAFARDFLPTTDFPPE
                     KVEAVAEAIADHMGLWRDEPLTVLESQVLWDADKLAKIGLTAVFQWMGGRLAGSEMRT
                     MADLIARGRKVDWQAKTVASMHTEPARRAAAARLAAYRDLYDALERELVGDDLLP"
     gene            complement(2699..2914)
                     /locus_tag="PLC06_12750"
     CDS             complement(2699..2914)
                     /locus_tag="PLC06_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91361.1"
                     /translation="MKKNKSDTEDDLRPEYDLKSLRVRKVGPKRTGFGGYVQLAPDVA
                     ELFPDAEAVNEALRFLIRITRESKMLP"
     gene            complement(2895..3173)
                     /locus_tag="PLC06_12755"
     CDS             complement(2895..3173)
                     /locus_tag="PLC06_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006508355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRO91362.1"
                     /translation="MDFEWDNEKAKINWSKHGIHFDEAETIFEDPLYIDFYDPDHSDN
                     EERYIIVGRSQQNRLLVVSYTERDNKIRLISAREATRREQMTYEEEQE"
     gene            complement(3333..4547)
                     /locus_tag="PLC06_12760"
     CDS             complement(3333..4547)
                     /locus_tag="PLC06_12760"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRO91363.1"
                     /translation="MNKKTVTDIDVNGKKAFVRVDFNVPLSSKDPAAHITVTDDTRIR
                     AALPTLNYLLDHGAALILASHLGRPKTADDVQFAMKPVAAKLQEVIGRPVKYVPAVMS
                     DEVKAAAAALRPGDILLLDNTRFHPGEKKNDAQLAADFASLADVYVDDAFGSAHRADA
                     SVDGAAYAMRAKGGPAVSGFLMNAEISALGVAVENPPHPYVAIMGGAKISDKIKLIEN
                     LLDKADKILIGGGMANTFLKAQGHNMGKSLVEEEALPEAARLLAMAADRLILPVDVVT
                     ATEVSADVPSDVEMIWALSPDRMALDIGPATVERFNAELQGAQLVIWNGPMGVFEVPQ
                     FAHGTNQLAQMLAAMVDNGAKVIIGGGDSAAAVRKAGLTDKMTHVSTGGGASLELLEG
                     RQLPGVVALDNR"
     gene            complement(4544..>4774)
                     /locus_tag="PLC06_12765"
     CDS             complement(4544..>4774)
                     /locus_tag="PLC06_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91364.1"
                     /translation="DPADRPGAWAAAVELIMGEQTNRGETQAAVGRHYRASLGAVAGR
                     VQQIRRALNIEGFDARYSDFSTTEIVYKESDE"
BASE COUNT          817 a         1600 c         1424 g          933 t
ORIGIN      
        1 caggacgggt tcgccgagct ggaagcccat ctgcgcgcct gccccgattg caaccgggag
       61 tggagcgggc tgatggcctt cgagctgctg ttccgccaga cgcccatcct catgcccgcc
      121 ctcgacttca ccgagcgcac cctggcgcgc ctgcccaatc gacgggcgcg ccgcgtcgcc
      181 atgggcaccg cctatgccgt tttgctattg agcggcatcg cccccctgct catcggcctg
      241 ttcctcgttg cccgctactg ggccgtcctc agcagccccg cgctgctcgg cggcgtgtgg
      301 gcctcggtgg ccggcgtcgg ccgcgccatc ctcacagtca tcgaagcgct gctggccggc
      361 gcaagccatt ttgtcatcga acaacccatc ctgatcggct ggttcatcat ccttgcgggg
      421 ctcgtattcc tgtggggcgg cgtttttcag cgattgctca tgcaacccgg cggcgccggt
      481 ctgcgtaatt agggttacag gacgcaggag taacggaaac ctgatctgca aggagcagtg
      541 aaatgaagaa aagtgtccta attccaatat tgctggccgc gctactcatc ctggcgctgg
      601 ccccggcaac cttcgccgcc ccgccggccg atacgatcat cgagtccggc gaggtcgtga
      661 acaatgacgt cgtcgtcttc gacggcgacc tggagattca atccggggcc accgtcaacg
      721 gcgacgtggc cgtgttcaac ggggatgccg tcatcgacgg ccaggtcaac ggcagcctga
      781 cgctgttcaa cggcgacctg caggccggag ccggcggcca catcaccggc gaatgcgtgc
      841 tgctcaacgg gcaggccgcc ggcgacggct tcgccgatcg caattccagc agctgcaccg
      901 ccattcaaag cctgaatgtc gccccgctgg ccgagctggg cccctggctc cgcgatctcc
      961 aggccatacc cggcgtgccc gaagtgccgg ccgtgcccga agtgccggtt gtgcccgccg
     1021 tgcccgaggt acccgccgtg ccggctgtgc ccggaataca ggccccccac agaggcaggg
     1081 gcggcttcgg cgccgaactg ttcggggtct ttgcgagcag cctgctgttc ggcttcctgg
     1141 ccctcatgac cgcggcgatt atgcccaatc aattacggca aatcacgacc accgcgcgcg
     1201 acaggtcgat ggtcagcggc atggccggcg cgttgacggc cgtggctgtg ccctcgctca
     1261 tcgtcctgct catcccgatc tcgatcatcc tgaccttcgt ctgcatcggc ctgctcggct
     1321 tcccgatcat gttcctgctg gggctggcgc tggtcgtcgg cagcttcctg ggctggatcg
     1381 ccgtcgggac atggctgggc atccgcatct tcggccgcgg caagagcggc gaccgcatcg
     1441 tgcgctcggc cgcgctgggc accgccctgc tgaccttcat caccggcgtg ctgggcttgc
     1501 tcacattcgg cgttgccggc ggaatcctgg tcttcatcgc cctgtccatc gggttgggcg
     1561 cggtggccct gacccagttc ggcatgaagc cctacccccg ccgctcgcga agcaacgcac
     1621 caggcagcgg cacgcccggc aacgacagca tcgaccccga caagtacgac aaggtgctaa
     1681 acaccctgcc gcccgaagaa gctgcggcgt agtcaggtta taaaaaccgg gtttcttctg
     1741 ggaaacccgg ttttttgttc ttgtagcgca gacatccctg tctgcaatcc ccatagcgca
     1801 gacaggaatg tctgctccta cggcagcaag tcgtcgccaa ccaactcccg ctccagcgcg
     1861 tcataaagat cgcggtaggc cgccagccgc gccgccgccg cccgcctggc cggctcggtg
     1921 tgcatcgacg cgaccgtctt cgcctgccaa tcgaccttgc ggccgcgtgc gatgagatcc
     1981 gccatcgtgc gcatctcgct ccccgccagc cggccgccca tccattggaa caccgccgtc
     2041 agcccaatct tggccagttt gtcggcgtcc cacaacacct gcgactccag caccgtcagc
     2101 ggctcgtcgc gccacaggcc catgtggtcg gcaatcgcct cggccaccgc ctcgaccttc
     2161 tccggcggga aatccgtcgt gggcagaaag tcgcgggcga aggcggcccc ggcgatgggg
     2221 tggcggttgc cgtcggtacg cttgcacacg tcgtggagcc aggcggccgc ttcgacgatc
     2281 tccgcgtccg cgccggtcag ctccgccagt cgcagcgcca gcgccactac cgccgtgaca
     2341 tgctcccagc gatagttgaa cgacacctcg gtcgtgccat agcgggtgag cagttcgccc
     2401 tgcgtagctt cgtacatggc ggcacgggcg gcttcgcgcc aggggggtgg tggtggaatc
     2461 gtcatgggat ctcgacgggg aactatagca gaagacgacg cagaagacag gttcagggat
     2521 ggcgggcgtt ggaggtgttg caggccgtcc agcggaccgg cagcgcggaa gatatcgacg
     2581 cggctttggc ggccgagcgg gtttggcagc ggcagcgatt gcgggagtta agcgaatggt
     2641 agcgcagggt tataagggag ttcggcatgg cgaacagtta acccacgtct ccaaagttct
     2701 atggcaacat cttgctctcg cgcgtaatac gaatcagaaa gcgcaaggct tcattaactg
     2761 cctccgcgtc aggaaacagt tccgccacgt cgggagccaa ttggacatat ccgccaaatc
     2821 cggttcgctt ggggccgact tttcttactc gcaggctttt cagatcgtat tcgggtcgaa
     2881 ggtcatcttc tgtatcactc ttgttcttct tcataggtca tctgttcccg gcgagtggct
     2941 tctcttgcac taatcaagcg gatcttattg tctcgctctg tataggatac aaccaataat
     3001 cggttttgtt gcgatcgacc gacgataatg tagcgctcct cattatctga gtgatcagga
     3061 tcgtagaaat caatgtatag tggatcttcg aagattgttt cagcttcatc gaagtgaata
     3121 ccatgtttcg accaatttat cttggccttt tcattgtccc actcaaaatc catactaaaa
     3181 gataacatgg attctcttga ttcgcattca gattcttgag actcgctccc ataagagcgg
     3241 gaaaagaaaa ggcccgacac aaagtcgggc cataaaggag tacataatcc ggatagcagg
     3301 tttcccggaa gaaaccttgt cactgctcgg tattaccgat tatccagcgc caccaccccc
     3361 ggcagctgcc gcccctccag cagctccagg ctggcgccgc cgccggtgct gacatgggtc
     3421 atcttgtccg tcagacctgc cttgcgcacc gccgcggccg aatcgccccc gccgatgatc
     3481 accttcgcac cattgtcgac catcgccgcc agcatctgcg ccaactgatt cgtgccgtgg
     3541 gcgaactgcg gcacctcgaa cacccccatc gggccgttcc agatgaccag ttgcgccccc
     3601 tgcaactcgg cgttgaaccg ctccaccgtg gccgggccga tgtcgagcgc catcctgtcc
     3661 ggcgacagcg cccagatcat ctccacatcc gacggcacgt cggccgacac ttcggtcgcc
     3721 gtcaccacat ccaccggcag aatcagccgg tcggcagcca tcgccagcaa ccgcgccgcc
     3781 tcgggcaggg cttcttcctc gacgagcgac ttgcccatgt tgtggccctg cgccttcagg
     3841 aaggtgttgg ccatgccgcc gccgatgagg atcttgtccg ccttgtccag caggttctcg
     3901 atgagcttga tcttgtccga gattttggcc ccgcccatga tagcgacata ggggtggggc
     3961 gggttctcca cggcgacgcc cagcgcggag atctcggcgt tcatcaggaa gccggagacg
     4021 gccgggccgc ccttggcccg catggcgtag gccgcaccgt cgacgctggc gtcggcccgg
     4081 tgggcgctgc cgaaggcgtc atcgacatag acgtcggcca gcgaagcgaa atcggccgcc
     4141 agctgcgcgt cgttcttctt ctcgccgggg tggaagcgcg tgttatccag cagcaggata
     4201 tcgcccggcc gcagcgccgc cgccgccgcc ttcacctcgt cgctcatcac cgccgggaca
     4261 tatttcaccg gccggccgat gacttcctgc aatttagcgg ccaccggctt catggcaaac
     4321 tgcacgtcgt cggccgtctt gggccgcccc agatgcgagg ccaggatgag cgccgcgccg
     4381 tgatcgagca gatagttgag cgtcggcagc gcggcccgga tgcgcgtgtc gtcggtgacg
     4441 gtgatatgag cggcgggatc tttgctgctc agcggcacgt tgaaatccac ccgcacaaaa
     4501 gccttctttc cgttgacgtc aatatcggta acggtcttct tgttcattcg tctgactcct
     4561 tatagacaat ttccgtggta ctaaagtcgc tgtagcgcgc gtcgaatcct tcgatgttca
     4621 gcgcgcgccg gatttgttgg actcggccgg ccaccgcccc cagcgaagcg cgataatggc
     4681 ggccgacggc ggcctgggtc tcgccacgat tcgtttgctc gcccatgatc agctccaccg
     4741 ccgccgccca cgcccccggc cggtcggccg ggtc
//
LOCUS       DAOMQG010000271         4717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7587_length_4717_cov_9.144144, whole genome shotgun sequence.
ACCESSION   DAOMQG010000271 DAOMQG010000000
VERSION     DAOMQG010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7587_length_4717_cov_9.144144"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="PLC06_12770"
     CDS             complement(<1..1366)
                     /locus_tag="PLC06_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRO91365.1"
                     /translation="MATPAIAPYGSWESPITTDLLLGGSVGLSQPCLEDDYVYWVEGR
                     PLEGGRNVIVRWSEAAGREDVTPPGFNARTRAHEYGGGDYVVRDGIVYFANFADQRVY
                     RQRPGETPEAITSEAALRYADFIVDAPRDRLICVREDHRSGDREAVNTLVAIPLDGGD
                     QRILAEGNDFYASPRLSPDGRRLAWLTWNHPNMPWDGTELWLADLDDNGSPIGTRLVA
                     GGPNESVFQPEFSPDGTLHFVSDRSGWWNLYRLRDGAAEALCPMAADFGMAQWIFGLG
                     TYDFESPTSIICAYTDRGKATIARLDVTSGRLTPLDLPYAIKYPSHGRIRVSGDACVF
                     IGGSPTLPSAVVRLDLATGAWQMLRSSSNIEIDEGYISIAQPIEFPTENGLSAHGYYY
                     PPRNRDFVAPSGERPPLIVTSHGGPTSATTGDLDLSRQFWTSRGFAILDVNYGGSTGY
                     GRTYR"
     gene            complement(1404..>4717)
                     /locus_tag="PLC06_12775"
     CDS             complement(1404..>4717)
                     /locus_tag="PLC06_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO91366.1"
                     /translation="DPAAEESRPVLATILFIDLAGHTSLVQGRDPEEIMEIVDQSLRR
                     LAMPIAHHGGRIVRFQGDGYKAVFGLPTARENDPDSAVRAALDILATAADIAVELEAE
                     RNLPGFRVRVGIATGRVLTGGGVEGEDTVAGLPVNLAARLESMAEPGTILIAHDTYRH
                     IRGVFDFLPLEPVMVRGFPEPVPIYRVLRRKPRSFRTRRRGVEGVETRMVGRDAELGT
                     LQEAFTWVIQNHERAAVVVIGEAGLGKSRLLYEFENWADLQPTDVQLYRGRGRLETQQ
                     QAYGLLRDLFAFRCGIFDDDAAEAAREKLVGIFRAELGAGDAVEMKAHLIGHLLGYDF
                     GGSPYVRPLLDNPRQLRDQAFTYLNEYFRAAAGRNPLLIMLEDLHWVDDGSLDAIASL
                     AAALEGYPAMLVGAARPTLYERRPDWLANRPAYRRLRLGLLEGAVGEQLVSEILQKMA
                     GVPDRLCALIVATAEGNPFYAEELIKMLIDEGVIIKGEEAWSVRMEQLADLHVPPTLT
                     GIIQARLESLPPLEKATLQRASVVGRLFWDAAVDYIGEGSVTTKTPWSALRRHELIYL
                     NEETAFEGTRQYTFSHALLRDVAYESVLRRVRWGYHHRVAEWLIMVGGDRVDEYAHLI
                     ANHFAQAGEDADEAEWQARAGKRAADRYAVTDALRALDRALELLPATETSRRFDLLLD
                     REKLYNIQAARDLQARDLGELERIVGGFDDLAARAEVALRWASYHLAMGDFKAAIASA
                     TQAAEQATSAGDITQQARAAILHGNSLRFLGDFAGTRARLSDGLRLAQAAQAKSVEMD
                     ALRSLGIAAQEQGDLSEQLLYFEGALALAREIGDLWGARRALNSLGIIEQNKGSYESA
                     QTYFEQSLVIARDIGDRLGENTVLGNLGVQATGLGQFPEARAYLEEALQIARDTDDVV
                     GVNTWLLNLAYIVAVLGDVDRALGLYDEVRGNVVAAGDRPLLGYVENGVGRVLLESGR
                     PTEAIPRLQQALALRESLEQPHLAAESRLLLAEALATTDDVSEAMATVEEGLAFLQVE
                     SLETTEDTARGLLAAYRVLSAAGDDRAGAILERAYDEVQAMVAPFDESSRRAYLENVP
                     CHREIVALREAMRSD"
BASE COUNT          862 a         1592 c         1464 g          799 t
ORIGIN      
        1 cgcgataggt acggccgtag ccggtgctgc cgccgtagtt gacatcgagg atggcgaagc
       61 cgcggctcgt ccagaactgg cggctcaggt ccagatcgcc ggtcgtggcc gaggttggcc
      121 caccgtggct ggtgacgatc agcggcggcc gctcccccga cggggccaca aagtcccgat
      181 tgcgcggcgg gtaataatag ccatgagccg ataggccgtt ctcggtgggg aactcgatcg
      241 gctgggcgat agagatatag ccctcatcaa tctcgatgtt gctcgaacta cgcaacattt
      301 gccaggcacc ggtggccaaa tcgagccgca cgacggccga cggcagcgtc ggcgacccgc
      361 cgatgaacac gcaagcgtcg cccgacaccc tgatccggcc atgggacgga tatttaatcg
      421 catagggcaa atcaagcggc gtcagtcggc cgctggtcac gtccaggcga gcgatggtgg
      481 ccttccccct gtcggtgtag gcgcagatga tcgaggttgg cgactcaaaa tcataggtcc
      541 ccaacccgaa tatccattgc gccatcccga aatcagcggc catcgggcag agcgcttcgg
      601 ccgccccgtc gcgcagccga tagaggttcc accagccgct gcggtcggag acgaaatgca
      661 gcgtgccgtc gggtgaaaat tccggttgga agacggactc gttcgggccg ccggccacaa
      721 ggcgcgtgcc aatcggcgag ccgttatcgt ccaaatcggc cagccacagc tccgtgccgt
      781 cccaaggcat gttggggtga ttccacgtca gccaggccaa ccggcggccg tcggggctga
      841 ggcgcggcga ggcgtagaaa tcattgccct cggccagaat gcgctgatcg ccgccatcga
      901 gcgggatggc gaccagcgtg ttgaccgcct cgcggtcgcc ggagcgatgg tcttcgcgca
      961 cgcagatcaa cctgtcgcgc ggcgcatcaa cgatgaagtc ggcatagcgc agcgcggctt
     1021 cggaggtgat cgcttccggg gtctcgcccg gccgctgccg atagacgcgc tggtcggcaa
     1081 agttggcgaa atagacgatg ccgtcgcgga cgacataatc gccgccgcca tactcgtggg
     1141 cgcgggtgcg ggcgttgaat ccgggtgggg tcacatcctc gcgcccggcc gcctccgacc
     1201 aacgcacgat aacgttgcgg ccgccttcca gcgggcgacc ctcaacccag tagacataat
     1261 catcttccag acaaggttgg ctcagcccaa ccgagccgcc gagcagcaaa tccgtggtga
     1321 tgggggattc ccaggaaccg tagggagcaa tcgcaggtgt agccatgggc caatgataat
     1381 gtccatcggg cgcggcggca aagttagtcg ctccgcatcg cttcccgcag cgccacgatc
     1441 tccctatgac agggcacatt ttccagatag gcgcgtcggg agctttcgtc gaagggcgca
     1501 accatggctt gcacctcgtc gtaggctcgc tcaaggatgg ccccggcccg atcgtctccg
     1561 gcggccgaca gcacgcgata ggccgcgagc aggccccgtg cggtgtcctc ggtggtctcc
     1621 aggctctcca cctgcaaaaa ggccagtcct tcttccacgg ttgccatcgc ctcgctgacg
     1681 tcatccgtcg tcgccagcgc ttcggccagc aagagccgac tctcggccgc cagatggggt
     1741 tgttccagcg actcgcgcag cgccagcgcc tgttgcaggc gcgggatcgc ttcggtcggg
     1801 cggccgcttt cgagcaacac tcgccccacg ccattctcga cgtagcccaa caacggccga
     1861 tcgcccgccg ccaccacgtt gccccgtact tcgtcgtaaa gccccagcgc gcggtcgaca
     1921 tcgccaagca cggccacgat ataggccagg ttaagcaacc aggtgttcac gcccaccacg
     1981 tcatcggtgt cgcgagcgat ctgcaaggcc tcttccagat aggcccgcgc ttcggggaac
     2041 tgacccagcc cggtcgcctg aacgcccagg ttgcccagca cggtattctc tcccagacgg
     2101 tcgccgatat cgcgagcgat gaccagggat tgctcgaagt aggtctgggc ggactcatag
     2161 gagcccttgt tctgctcaat gatgcccagg ctgttgagcg cacgccgtgc cccccacagg
     2221 tcaccgatct ctcgcgccag cgccagcgcg ccttcaaaat acaagagttg ctcgctcagg
     2281 tcgccctgct cctgggcggc aatccccagc gagcgcagcg cgtccatctc gacgctcttc
     2341 gcctgggcgg cttgcgccag ccttaaccca tcgctcaacc tggcccgcgt gccggcaaaa
     2401 tcgcccagaa agcgcaggct gttgccgtga aggatggcgg cgcgagcctg ttgggttatg
     2461 tctccggcac tggttgcctg ctcggccgcc tgggtcgccg aggcaatcgc cgccttaaaa
     2521 tcgcccatgg ccagatggta gctcgcccag cgcaatgcaa cctcggcgcg cgcggccagg
     2581 tcatcgaacc cgccgacgat ccgctccagt tcgcccaaat cgcgggcctg aagatcgcgg
     2641 gcggcctgaa tattgtaaag cttctcacga tcaagcagaa gatcaaatcg ccgcgacgtc
     2701 tcggtcgccg gcaatagctc cagcgcgcga tccagagcgc gcagggcgtc agtcaccgca
     2761 tagcgatcag ccgcccgctt gccggcgcgc gcctgccatt cggcctcgtc tgcgtcctcg
     2821 ccggcctgag cgaaatggtt ggcgatcaga tgggcgtatt catccacccg gtcgccgccg
     2881 accatgatta gccattcggc cacgcgatga tgatagcccc accgaactcg ccgcaacacg
     2941 ctctcatagg ccacatctcg caggagcgca tgactgaatg tgtattgccg ggtgccttcg
     3001 aaggccgtct cttcgttcag atagatcagc tcgtggcggc gcagcgcgga ccagggcgtc
     3061 ttggtcgtga cggaaccctc gccgatatag tccacggctg cgtcccagaa caggcggccg
     3121 accaccgagg cgcgctgcag ggtcgccttt tccagcggcg gcagcgactc gagccgcgcc
     3181 tggattatgc cggtcagcgt cggcggcaca tgcagatcgg ccaactgctc catcctgacc
     3241 gaccaggcct cttccccctt gatgatgact ccctcatcga tgagcatttt gatcagctcc
     3301 tcggcgtaga aaggattacc ctcggccgtg gcaacgatta gcgcgcacag ccgatcgggg
     3361 acgcccgcca tcttctgcaa tatctccgat accagttgct ccccgaccgc accctcgaga
     3421 agccccaacc tgagccgccg atatgccggg cggttggcca gccagtcggg acgtcgttcg
     3481 tagagcgtcg ggcgggcggc acctacaagc atggccggat acccctccag cgccgctgcc
     3541 agcgaggcga tcgcgtcgag cgagccgtca tcgacccaat gcaggtcctc gagcatgatg
     3601 agaagcggat tgcgcccggc ggcggcccgg aaatattcgt tcagatatgt aaaagcctga
     3661 tctcgtagct gtcggggatt gtccagcagc ggccgcacgt agggggagcc gccgaaatca
     3721 taccccagca gatgaccgat gagatgggct ttcatctcta cagcgtcgcc tgcgcccaat
     3781 tcggcccgga agatgccgac cagtttttcg cgtgccgcct cggccgcgtc gtcgtcaaag
     3841 atgccgcagc gaaaggcgaa cagatcgcgc aataggccat aggcttgctg ttgcgtctcc
     3901 aggcggccgc gtccgcgata gagttgcacg tcggtcggtt gcaggtcagc ccaattctca
     3961 aactcataga gcaggcgtga tttgcccagc cccgcctcgc cgatgacgac caccgcggcg
     4021 cgctcgtgat tttgaatgac ccacgtaaac gcttcctgca gcgtgcccaa ctcggcatcg
     4081 cggccgacca tgcgcgtctc gacaccctcc acgccgcgcc ggcgagtgcg gaaagagcgt
     4141 ggtttccggc gcagcacccg atagattggc acaggttccg ggaaccccct gaccatgacc
     4201 ggctccagcg gcagaaagtc gaacacgccg cgaatgtgcc ggtaggtgtc gtgggcgatg
     4261 aggatcgtcc ccggctcggc catactctcc agacgtgcgg ccagattgac cggcagcccg
     4321 gcgacggtat cctcgccctc cacgccgcca ccggtcagca cccggccggt ggcgatgccg
     4381 acccgcaccc ggaagccggg caggttgcgc tcggcctcca gttcgacggc gatatcggcc
     4441 gctgttgcca aaatgtccag cgcggcgcga acggcgctgt cgggatcgtt ctcacgcgcc
     4501 gtgggcaggc cgaaaacggc cttgtagccg tcgccctgga agcgcacgat gcgaccgccg
     4561 tggtgggcga tcggcattgc caatcgtcga agcgactgat cgacgatctc catgatttct
     4621 tccgggtcac gcccctggac gaggctggta tgaccggcca ggtcgatgaa caggatggtc
     4681 gccaacaccg ggcgcgattc ttcggccgcc ggatccg
//
LOCUS       DAOMQG010000272         4708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7603_length_4708_cov_8.728132, whole genome shotgun sequence.
ACCESSION   DAOMQG010000272 DAOMQG010000000
VERSION     DAOMQG010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7603_length_4708_cov_8.728132"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..581
                     /locus_tag="PLC06_12780"
     CDS             288..581
                     /locus_tag="PLC06_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HRO91367.1"
                     /translation="MTQKTVETQPWDVVDHLTTEEDMIAYLDAAFEDGDPALIVAALG
                     DIARAKGMTQVAREAGLGRESLYKALSPTGNPEFTTILKVLNALGLRFHVESV"
     gene            654..2300
                     /gene="hutU"
                     /locus_tag="PLC06_12785"
     CDS             654..2300
                     /gene="hutU"
                     /locus_tag="PLC06_12785"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRO91368.1"
                     /translation="MTRTIRAPRGTTLHCKGWLQEAAYRMIQNNLDPEVAYDPEHLIV
                     YGGRGRAARNWEAFDAILRSLTELEDDETLMIQSGKPVAVFRTHADAPRVLIANSNIV
                     PHWATQENFDRWEAEGLIMYGQMTAGSWIYIGTQGILQGTFETFAEAARQAGWPSLKG
                     KWALTAGLGEMGGAQPLAVTMNDGVALVVEVDEARARRQLEHRYIDEVSEDTEDALRR
                     VRHYTEAGEARSIGLIGNAAAVFPDLLARGVLPDLVTDQTSAHDFLAYFPDGMSFEDG
                     RALRDRDPVEFARLSAASMARHCAAMVEFQRRGSIVFDYGNNLRQRAYDEGVTDAFSY
                     PGFVPAYVRPLFCEGKGPFRWVALSGDPEDIRVTDEAILELFPDDAHLHRWIRMAQER
                     VQFQGLPARICWLGYGERHRAGLRFNELVASGAVKAPIVIGRDHLDAGSVASPNRETE
                     GMRDGSDAIADWPILNALVNTAAGATWVSFHHGGGVGIGYSLHAGQVIVADGTPAAAR
                     RLERVLTVDPGMGVARHVDAGYERAIEVARERGVKIPMLK"
     gene            2352..3677
                     /locus_tag="PLC06_12790"
     CDS             2352..3677
                     /locus_tag="PLC06_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRO91369.1"
                     /translation="MIYSLRLLLLLALPMAVLLVTTQRPLIAEGTAPLLPPSTFLPLH
                     EVGDEWGADEYAPAIAFGDIDGDGRDEIAISRRATAGARVLVLDDAMAGFVPLWSFGE
                     GWGVASWVTALAFGNIDDDPAEELTFTRMAAVNERVWVVDDAAAGFAFLARFGEDWPD
                     TVSAVGVALGDIDGDGRDEIGVITDTSASDRVHVFDDAAAGFAPLWDGDETWGAASVA
                     TSIAFGDVDGDGFDELAVTRKHDINARLFLYDDAAPSTAQGFGLLWQYGEGWGPGSYA
                     TGVAFGNVDGDPAEEIGLTRQATLNERAYIFDDAAASYVTLQTFGESWAANAWPTAIA
                     FGDVDSDGRDEVALSRVATINARVYVHDDAAPDKSRPAFKLLWGGGAEWPGEMYATAV
                     AFGNVDANPETELGLGRFAAEGSRAYLLGRGWAVGLPYVSEGRIMTADR"
     gene            complement(3747..4604)
                     /locus_tag="PLC06_12795"
     CDS             complement(3747..4604)
                     /locus_tag="PLC06_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO91370.1"
                     /translation="MIPYDDFGGRGPVLHFSHPNAYTPRCFRRFLACLTERHHVIAGR
                     HRPLWYLSPGAPSVPGPETFNDWNLIADDLLRFLDQQRLEGIVGIGHSLGAVATMKAA
                     LREPERFRALALIEPVFLPPPVLDAIRANPSAAAEQPFVLAARRRRDRWPDRQAAFDR
                     FREKSVFAGFPDDSLWDYVNEGLHDDPTTGEVALSFPREWEAAFYAHPPLDAWDEIPR
                     LTLPTLALRGSESDTLFPGPWAQWQTLQPSATFVEMSGVGHMLTLEAPEETAAVVLHW
                     LDDRVGSEQ"
BASE COUNT          854 a         1463 c         1531 g          860 t
ORIGIN      
        1 gattcggaca tatttctccc tgtgataaaa tgtggcgcag atatgatctg atagtggatc
       61 atgggtatca atcagacgaa ctatactgtc gctgcttgcc ggtgacaaac ggtcacgaag
      121 tcaaccgtaa tctattcacg agcatcgaaa tttacagctc ctattgttat tcagtatccc
      181 ataagataca atagccgtga ccatcgaaat tcgtcagacg aaggtttatc agaagtggtt
      241 tgccaaatta caagatcggc aggcacgtgg gctaaaggag ccttaagatg actcaaaaaa
      301 cggttgaaac gcagccgtgg gatgtggtcg atcatttgac gacggaagaa gatatgatcg
      361 cctatctgga cgctgctttt gaagacggcg atccggcact gatcgtcgcc gcgctcggcg
      421 atattgctcg cgccaagggc atgactcagg tcgcgcgcga agcagggtta ggtcgcgaga
      481 gcttatacaa ggcattgtcg ccaaccggca accccgaatt cactaccatc ctcaaggtgc
      541 tcaacgcctt gggcctgcgc tttcatgtcg aatcggtata gttaaggaca ttagctggtt
      601 aaaacatctc aattctttac ttgtctttcg tcactcatca ttcgtaactt accatgaccc
      661 gcaccatccg cgccccacga ggcacaaccc tacactgcaa aggctggctg caagaggccg
      721 cctaccgtat gatccagaac aatctcgacc ccgaggtcgc ctatgacccg gagcatctga
      781 tcgtctacgg cggccgcggc cgggcggccc gcaactggga ggcgttcgac gccatcctgc
      841 gcagcctgac cgagctggag gacgacgaga cgctcatgat ccagagcggc aagccggtgg
      901 ccgtcttccg cacccacgcc gacgcgccgc gcgtgctcat cgccaacagc aacatcgtcc
      961 cccactgggc cacgcaggag aacttcgacc gctgggaggc cgaggggctg atcatgtacg
     1021 gccagatgac cgccggcagc tggatctaca tcggcacgca aggcatcctg cagggcacct
     1081 tcgagacctt cgccgaagcc gcacgacagg ccggatggcc ctctctcaag ggcaagtggg
     1141 cgctgacggc cgggctgggc gagatgggcg gggcgcaacc gctggccgtg acgatgaatg
     1201 acggcgtggc gctggtcgtc gaggtcgatg aggcccgcgc ccgtcgccag cttgaacatc
     1261 gctacatcga cgaggtgagc gaggacaccg aggacgcgct gcgccgcgtg cgccattata
     1321 ccgaggccgg ggaggcccgc tccatcggcc tcatcggcaa cgccgcggcc gttttccccg
     1381 atttgctggc ccgcggcgtg ctgcccgacc ttgtcaccga ccagacctcg gcccacgact
     1441 ttctggccta cttccccgac ggcatgagct tcgaggacgg ccgcgccctg cgcgaccgcg
     1501 atccggtcga attcgcgcgg ctgtccgccg cttcgatggc ccgccactgc gcggccatgg
     1561 tcgaattcca gcgccgcggc tccatcgtct ttgactacgg caacaacctg cgccaacgag
     1621 cctatgacga gggcgtgacc gacgccttca gctatcccgg cttcgtgccc gcctacgtgc
     1681 ggccgctgtt ctgcgagggc aaggggccgt tccgctgggt ggccctcagc ggcgacccgg
     1741 aggatatccg cgtcaccgac gaggccatcc tggagctgtt ccccgacgac gcccatttgc
     1801 atcgctggat tcgcatggct caggagcgcg tgcagttcca ggggttgccg gcgcgcatct
     1861 gctggctggg ctatggcgag cgccatcggg ccgggctgcg cttcaatgag ctggtggcgt
     1921 ccggcgcggt gaaggccccc atcgtcatcg gccgcgatca tctcgacgcc ggttccgtag
     1981 ccagccccaa ccgcgagacg gagggcatgc gcgacggcag cgacgccatc gccgactggc
     2041 ccatcctcaa cgcgctggtc aacaccgccg ccggagcgac gtgggtcagc ttccatcatg
     2101 gcggcggcgt cggcatcggc tatagtctgc acgcgggaca ggtgatcgtc gccgacggca
     2161 cgccggcagc ggcgcggcgg ctggagcggg tgctgaccgt cgatccgggc atgggcgttg
     2221 cccgacacgt ggacgcgggc tatgagcggg ctattgaagt ggcccgcgag cgtggcgtca
     2281 agataccgat gctgaaatga cgaccggaaa ccgggttttt gaaaaagccc gggttctgtt
     2341 tttaacccat tatgatctat tccctacgcc tgcttctcct gctcgccttg ccgatggcgg
     2401 ttttgctggt gacaacccaa cgacctctaa tcgctgaagg gacggcgccc ttgttgcccc
     2461 cgtctacctt tctgcctctt catgaagttg gtgatgagtg gggtgcggat gagtatgccc
     2521 cggctattgc cttcggcgat atcgacggcg acggccgcga cgagatcgcc atctcgcgcc
     2581 gagccacggc cggcgcgcgc gtgctggtgc tcgatgacgc aatggccggc ttcgtgccgc
     2641 tgtggagctt cggcgagggg tggggcgtgg cgtcgtgggt cacggcgctg gccttcggca
     2701 acatcgacga tgatccggcc gaggaactga cgtttacgcg catggccgct gtcaacgaac
     2761 gggtgtgggt ggtcgatgac gccgccgccg gattcgcctt tctggcgcgt tttggcgagg
     2821 attggccgga taccgtctcg gccgtgggcg tggccctcgg cgatatcgac ggcgatggcc
     2881 gcgacgagat tggcgtgatc accgacacca gcgcgagcga tcgcgtccat gtgttcgacg
     2941 atgcagccgc cgggttcgct ccgctctggg atggggacga gacatggggc gcggcgtcgg
     3001 tggccacgtc gattgctttc ggcgatgtgg atggcgatgg gttcgacgag ctggctgtca
     3061 cgcgcaagca tgacatcaac gcgcgcctgt ttctctatga cgatgctgca ccttccactg
     3121 cacagggttt cggacttcta tggcaatatg gtgaaggttg ggggccgggt tcctacgcta
     3181 ctggggtggc ttttggcaac gtcgacggcg atccggccga ggagatcggc ctcacccgcc
     3241 aggcgacact aaacgagcga gcttacatct ttgatgatgc agctgcgagc tacgtcacat
     3301 tgcaaacgtt cggcgagtca tgggcggcca atgcctggcc taccgcgatc gccttcggtg
     3361 atgtagacag cgacggccgc gacgaggtgg ccctgtcgcg cgtggcgacg attaacgcgc
     3421 gcgtctacgt ccacgacgac gccgcgccgg acaaaagccg gccggcgttc aagctgttgt
     3481 ggggcggcgg cgcggagtgg ccaggcgaga tgtacgccac ggccgtggct ttcggcaatg
     3541 tggatgcaaa cccggagacg gaacttggcc tgggtcgatt tgctgccgag gggtcgcgcg
     3601 cctacctgct gggtcgcggt tgggccgtgg ggctgcctta tgtgagtgaa ggacggatca
     3661 tgactgccga ccgctgacca ccggcaaccg cacaccgccc tactgccttg ctgttctact
     3721 gacctactgc tctactgacc tactgctcac tgctcactgc ccacccgatc atccagccaa
     3781 tgcagcacca ccgcggccgt ctcttccggc gcttccagcg tcagcatgtg gccgacgccg
     3841 ctcatctcca cgaaggtggc cgacggctgc aaggtttgcc attgcgccca cgggccgggg
     3901 aagagcgtgt ccgattcgct gccgcgcaaa gccagcgtgg gtagcgtcaa tcgggggatt
     3961 tcatcccatg cgtcaagcgg cgggtgggca tagaaggccg cttcccattc gcgcgggaag
     4021 ctcagcgcga cctcgcctgt ggtcgggtca tcatgcagcc cttcattgac ataatcccaa
     4081 agcgaatcat caggaaaacc agcgaagacc gacttttcgc ggaaacggtc aaatgcggct
     4141 tgccggtcag gccagcggtc gcggcggcgg cgggcggcga gcacgaacgg ctgctcggcc
     4201 gcggctgatg ggttagcgcg gatggcgtcg agcaccggcg gaggcaaaaa caccggctcg
     4261 atgagcgcca gcgcccggaa gcgctccggc tcgcgcaagg cggccttcat cgtcgccacc
     4321 gcgcccagag agtggccgat gccgacaatg ccctccagcc gctgctgatc gagaaagcgc
     4381 agcaggtcgt cggcgatgag gttccaatcg ttgaatgtct ccggcccggg cacggacggt
     4441 gcgccgggcg aaagatacca cagcggccgg tgacgcccgg cgatcacatg gtggcgctcg
     4501 gttaaacatg ccagaaagcg ccggaagcaa cgcggcgtgt aagcgttggg gtgggagaag
     4561 tggagaacgg ggccgcggcc gccgaaatcg tcgtagggga tcatttgcag tatccgggtt
     4621 atgctttcca gtcgcccacc caatgaacac cgtccagccc ggcggcctgg gcggcgttgg
     4681 ccaggcggcg atcggccgtc cagaatgg
//
LOCUS       DAOMQG010000273         4703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7621_length_4703_cov_9.413511, whole genome shotgun sequence.
ACCESSION   DAOMQG010000273 DAOMQG010000000
VERSION     DAOMQG010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_4703_cov_9.413511"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1840
                     /locus_tag="PLC06_12800"
     CDS             275..1840
                     /locus_tag="PLC06_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91371.1"
                     /translation="MTTELLPSGTRRIDRLEMIGITKRFPGVLANNHINFDVLSGEVH
                     ALLGENGAGKSTLMKILYGLQPPDEGEIRLNGEAVDIHSPADAIRLGIGMIHQHFMLV
                     PTLTVTENVALGLKSSRGMRTDLDKVEARINELAELYGLRVDPDALVWQLSVGQQQRV
                     EILKALYRGAALLILDEPTAVLTPQEVDEFFLTLEQMKKDGHALIFISHKLHEVIQLC
                     NRITVLRDGHVIGTRPTAGVTKQQLAEMMVGRPVGMQPAKPPMKLGDVRLSLRNVEAL
                     NDRGMPALRGVSFDLRGGEIVGLAGVSGNGQRELAEVIAGLRPLAGGTVTIGGSSIAE
                     ASPRAIMDRGLSYIPEERMRDGMIRDFTVSENLILREFDKPPFARAGFLDPGAIKRNS
                     TELVRKFNVKTPTLETPVKNLSGGNIQKLVLARELSRKPQAIVAAQPTRGLDIGATEY
                     VHAQLLAQRDLGTATLLISEDLDEILALSDRILVIFHGEIMGDVPRAEATAEKLGLMM
                     AGESHQPEESPVS"
     gene            complement(1872..2969)
                     /locus_tag="PLC06_12805"
     CDS             complement(1872..2969)
                     /locus_tag="PLC06_12805"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRO91372.1"
                     /translation="MKAFVTGGTGFIGRAVMRKLIERGYSVNALARSESGAERLRAIG
                     AAVSLGDITDRESMREGMLGSDVVFHIAGLYQFTPEALARAESVNVDGTRNVLELAVE
                     LGIPRIVYTSTVAVFGDTRGEIPDETYYAGGPFLSEYDRTKWVTHYEVAEPLIAKGAP
                     IVIVMPGGVYGPGDSSMIAETMRLFYRGMLPVLPGPETVLTYAYVDDIAEGHILAAEK
                     GRIGESYILTGPAVPLGEMVDFWAQLTGKPRPAAHIPARFARGLAPVANRAQPALSLP
                     QAFSGAAFSSLGAAYAGRSDKARAELGWRPRPIQAGMRETFEWIAATEPAGMGDREKR
                     IGAIILIAAVVVFLLWLIGRGKRRPPSTSED"
     gene            3166..4683
                     /locus_tag="PLC06_12810"
     CDS             3166..4683
                     /locus_tag="PLC06_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRO91373.1"
                     /translation="MDDSVTDIINSRHGQATRIITITMIVLVVLQAAIFMLLFAVFGR
                     QMAVEWLLELVLAILFTLLMGAIALYIVGQARQAVSRDVALAVGQEMRELKSANDRAQ
                     SLQRMASTLSATLSFERVVEQSLDVCSLALEEMGIPRDSLVGAVFLYEGGVLRPIARR
                     RFLGDDSKQVIAGKQGVVGAALSQAEPVITNNPQDDPELRAYAAFRECLTAACVPLRA
                     GFQLFGVMVIGADTAVHFDRRHIDLFKSVGDQAVIALQNAQLYQRLEAEKQRLIEADE
                     EARKELARDLHDGPTQSIAAIAMRMGFARTMIDRDPARAKEELLKVEALAKHTSAEIR
                     GMLFTLRPLILEAQGLGPAIDALLRRYSENDGIEMRLIGAEVGTLLSKQAQVVAFAII
                     EEALNNARKYAKASLIEVRFWQTDGLFAASVRDDGVGFDTYDINRDYSTRGSLGMVNM
                     RERAERIDGSLRVDSTPASGTVVTLVVPLDKHGAAVEMDEEEPASTEPQLVNAGP"
BASE COUNT          948 a         1421 c         1437 g          897 t
ORIGIN      
        1 tcaacgtctg gaccggcccg atcaacctgc aggacggcac ttcctatatc gccgatggcg
       61 cggccgcgac cgacgaagaa atctggtatt tgccgcaact gcttgaaggc atggaaggcg
      121 ccagccagta accgttcaga tagcatgagg cgcatccgca tgtgcgcttg ctgaagggcg
      181 tgggggcggg tgtgacggac agcctgtcgc accccccccc accagaagaa ctgaaccctt
      241 cctggaacat tagattcaac agcctgaggc ccaaatgacc acagaactcc tcccatccgg
      301 cacccgccgc atcgatcgtc tggagatgat cggcatcacc aaacgcttcc ccggcgtgct
      361 ggccaataat cacatcaatt ttgacgtact ctccggcgaa gtccacgccc tgctgggcga
      421 aaacggcgcg ggcaaaagta cgctgatgaa aatcctctac ggcctccagc cgccggatga
      481 aggagaaatc cgtctaaacg gggaagcggt agatatccac tctccggccg atgccattcg
      541 cctgggcatt ggcatgatcc accaacactt catgctcgtg ccgacattga cggtcaccga
      601 gaacgtggcc cttggcctca agtcatcgcg cgggatgcgc accgatctgg acaaggtaga
      661 agcccgcatc aatgaattgg ccgagctata tggcttgcgc gtcgatccgg acgcactggt
      721 gtggcagcta tcggtcggcc agcagcaacg ggtagagatt ctaaaagcgc tctatcgcgg
      781 cgcggccttg ctcatcctcg acgagccaac ggccgttttg acgccgcagg aagtggatga
      841 gttcttcctg acactggagc agatgaagaa agacggccat gccctgatct ttatctccca
      901 caagttacat gaggtgatcc agttgtgtaa ccggatcact gtgctgcgcg acggccatgt
      961 gatcggcacc cggccgacgg caggcgtgac caagcagcag ttggccgaga tgatggtcgg
     1021 ccgtccggtc ggcatgcagc cggccaaacc gccaatgaag ctgggcgacg tgcgtctcag
     1081 ccttcgtaat gtggaagccc tgaatgatcg cgggatgcct gccttgcgag gcgtctcgtt
     1141 cgatctgcgt ggcggagaga tcgttgggct ggcgggcgtg tcgggcaacg gccaacgcga
     1201 actggccgag gtgatcgccg ggctgcggcc gttggccggc ggaacagtga ccatcggcgg
     1261 cagttccatc gctgaagcat ctccccgcgc catcatggat cgaggtctct cctatatccc
     1321 cgaagagcgc atgcgcgacg gtatgatccg cgacttcacc gtttcggaga atctgatcct
     1381 gcgcgaattc gacaagccgc cttttgcccg ggccggtttt ctggatcccg gggccatcaa
     1441 gcggaattcg accgaactgg tcagaaagtt caacgtcaag acgccaacac tggagacgcc
     1501 ggtcaagaac ctgtccggcg gcaatattca gaaactggtg cttgcgcggg agctatcgcg
     1561 caagccacag gccatcgtag ccgcccagcc gacgcgcggt ctggatatcg gcgcgacgga
     1621 gtatgtccac gcccaactgc tcgcccagcg tgatctgggt acggcgacgc tgctcatttc
     1681 cgaggatctg gatgaaattc tagcgctgtc cgatcgcatc ctggtgatct ttcatgggga
     1741 gatcatgggg gacgttcccc gcgccgaggc gacggccgag aagctgggcc tgatgatggc
     1801 cggagagtca caccagcctg aggaatcgcc tgtttcctga gtgcaagacg caggcgctgt
     1861 cggaacgctg ttcagtcttc cgacgtagag ggcggccgac gcttgccgcg gccgatcagc
     1921 cacagcagga ataccacgac ggcggcgatc aggatgatcg cgcctattcg tttctcacga
     1981 tcacccatgc cggccggttc ggtggcggca atccattcaa acgtctcccg catcccggcc
     2041 tgaataggac gcggccgcca acccaactct gcccgtgcct tgtcggagcg cccggcatag
     2101 gccgcgccca gcgagctgaa cgccgctccg ctgaacgcct ggggcaagga aagagccggt
     2161 tgggcacgat tggcgacagg agccaggcca cgagcaaatc gcgccgggat gtgggcagcc
     2221 gggcgcggct tgcccgtcag ttgtgcccaa aaatccacca tctcgcccag cggcacggcc
     2281 ggtccggtca ggatataact ctcgccgatg cgccccttct cggcggccag gatgtgaccc
     2341 tcggcgatat cgtcgacgta ggcataggtc agaaccgttt cggggccggg caagacgggg
     2401 agcatccctc gatagaacag gcgcatcgtc tcagcgatca tgctgctatc gccggggccg
     2461 tagacaccgc ccggcatgac gatgacgatc ggcgcgccct tggcgatgag cggttcagcc
     2521 acctcgtagt gggtcaccca cttcgtccgg tcgtattcgg acaggaacgg cccgccggcg
     2581 tagtaggttt cgtccggtat ctccccccgt gtatcgccaa atacggccac cgtgctggtg
     2641 tagacgatgc gcggaatacc taactcgacg gccaactcca ggacattgcg cgtgccatcg
     2701 acattgaccg attctgcccg ggccaatgct tccggcgtga actggtatag gccggcaata
     2761 tggaaaacga catcgctgcc gagcatgcct tcgcgcatgg attccctgtc ggtgatgtca
     2821 ccgagactca cggctgctcc tatggcacgc agccgctccg cgccgctctc cgaacgagcc
     2881 agcgcattga ccgagtagcc gcgctcaatc agtttacgca taactgctcg cccgatgaat
     2941 cccgtcccgc cggtgacaaa tgctttcatg ccgcttcctc caatggtatg ccgcaggatt
     3001 atactcgtga acgaggagat ggtgaacgaa ggatataatc tgatatagta ggaagccaat
     3061 agagcgttca tttgaccgta aggttgttgt tctataatta cctcggtggg ttaaaagtcg
     3121 ggcgggtccg tcacggcagc ttcaaccttc taatcgcctc tcatcatgga cgattcagtt
     3181 accgacatca tcaatagccg gcatggccag gcgacacgta tcataacgat cacgatgatc
     3241 gttctggttg tgctccaggc agcgatcttc atgctgttat tcgccgtctt tggtcggcag
     3301 atggccgtgg agtggttgct ggagctggtg ctggcgatcc tgtttacgct gctgatggga
     3361 gccattgcgc tctacatcgt ggggcaggca cgacaggccg tctcacgcga tgtcgctctg
     3421 gccgtggggc aggaaatgcg cgaactaaaa tcggctaatg accgggccca atcattgcaa
     3481 cgaatggcct cgaccctcag cgcgaccctg agtttcgagc gcgtagtgga acaatcgctg
     3541 gacgtatgca gcctggctct ggaagagatg ggcatcccgc gcgattcgct ggtgggcgcg
     3601 gtgttcctgt atgaaggtgg cgttttgcga cccatcgccc ggcgtcgctt cctgggtgat
     3661 gacagcaagc aggtgatcgc cggtaaacag ggcgtagtgg gcgccgcgct tagtcaggct
     3721 gagccggtaa tcaccaataa tccccaggac gaccccgagc tgcgcgccta tgcggcattt
     3781 cgtgaatgcc tgacggccgc ttgcgtgccg ctgcgggccg gattccagct gtttggtgtg
     3841 atggtaattg gcgccgatac ggccgtccac tttgatcgcc ggcacatcga tctgttcaag
     3901 tcagttggcg atcaggctgt catcgccctg caaaacgccc agctatacca gcgcctggaa
     3961 gcagaaaagc agcgactcat cgaggctgat gaagaggcgc gcaaggaact cgcccgcgat
     4021 ctccacgatg gcccgacgca gagcatcgcc gccatcgcca tgcgcatggg gttcgcccga
     4081 acgatgatcg atcgcgatcc ggcgcgggcc aaagaggagc tgctcaaggt cgaggcattg
     4141 gccaaacata cgtcggcgga gatccgcggc atgctgttca ccttgcggcc gctgattctg
     4201 gaggcgcagg gccttggccc ggcaatcgat gccctgctgc gtcgctatag cgagaacgac
     4261 ggcatcgaga tgcgcctgat tggggccgag gtcggcacat tgctgagcaa acaggcgcaa
     4321 gtggtggctt ttgccatcat cgaagaagcg ttgaataacg cccgtaaata tgcaaaggcg
     4381 tcgctcatcg aggtccgttt ctggcaaacc gacggcttat ttgcggcgtc agtgcgtgac
     4441 gacggtgtgg gattcgatac ttacgatatc aatcgcgatt acagcacacg cggcagcctg
     4501 ggcatggtca acatgcgcga gcgggccgag cgcatcgacg gctcgctgcg cgtcgattcc
     4561 actcccgctt ccggcacggt cgtgacgctc gtcgtcccgc tcgacaaaca tggcgcggcg
     4621 gtcgagatgg acgaggaaga gccggcttcc accgaaccgc aattggtcaa tgccggcccc
     4681 tgaaccccct gtgcgctcgc tca
//
LOCUS       DAOMQG010000274         4701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7630_length_4701_cov_6.528842, whole genome shotgun sequence.
ACCESSION   DAOMQG010000274 DAOMQG010000000
VERSION     DAOMQG010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7630_length_4701_cov_6.528842"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLC06_12815"
     CDS             <1..759
                     /locus_tag="PLC06_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HRO91374.1"
                     /translation="FAHTGHRPWPLPRGRWTWRQSWRDLLFAHWPVPTEAIRSLVPAG
                     LAVQEFDGTSWIAAVPFRMAGVMRRPLPDLPWVSAFPQLNVRVYVEAEGKPGVFFLSI
                     DAANPLVAAIARTLFHVPYFWADMRLERRGGWIQYESVRRGGKGEGEKGGRGEGEAVF
                     RGRYRPTSEVYAAAPGTLEHFLTERYCLYAVSADGVVRRTEVHHAPWPLQRAEAEIET
                     NTMTAAGGVAVDDPPALLHFSRRIDVVVWGSEGV"
     gene            898..3678
                     /locus_tag="PLC06_12820"
     CDS             898..3678
                     /locus_tag="PLC06_12820"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRO91375.1"
                     /translation="MTPEVLARQQIDQLLQHAGWEVQDRARMSLFGRPGRGVALREAV
                     MTTGEADYLLLVDGRALGVIEAKRTGHTLSHVEEQSGRYAVGLPPGVKPWLPGRPLPF
                     RYESTGVETFFTNGLDPAPRSRPVFAFHRPATLAEWADEPLTLRARLRRMPPLITDAL
                     WPPQIAAIANLERSLIEDRPRALIQMATGSGKTFTAVNFVYRLIRHAGARRVLFLVDR
                     NNLGRQAYTEFTNFITPDDGRKFEDLYNIQHLTGKIIDPVNRVVISTIQRLYAMLRDE
                     ELEEDADLGSTYELEERGQLESASRTVSFTGRLPIEQFDFIIVDECHRSIYTVWRQVL
                     EYFDASLIGLTATPGKQTIGFFRQNMVSEYTHEMAVADGINVAGDVYRIRTRIGDEGS
                     TVEAGYYVDRLDKLTRARRAELLDEDLTYTADQLDRNVASESQIRTVIRAFRDALFTE
                     LYPDRTDQVVPKTLIFAKDDNHAETIVRVAREEFAKGNDFCQKITYRAERNPEDILKD
                     FRNSYNPRIAVTVDMIATGTDVRAIEILLFMRRVRSRGYFEQMRGRGTRIISSDDLQA
                     VTRDARHKTHFILIDAVGLVEAGMAETTRTLERKRSVPFKQLLEGIAFGADDEDTVSS
                     LAGRLARLQHELTAADESRIREATGGRDMAGLIGLLFDALDPDRQIAQARAAGYPAPG
                     EAEIEAAAAELRGIAVAPFRDSPDLREALLTAQGRNEIIIDTVSADEVTVRGFDAAAT
                     DRLRKTVDDFRQFIEANKDEIAALQILYNEPYGRARPTRAQLKELAAALQRPPHLWTE
                     AALWNAYAAVERDRVRGAGETRVLTDLVALVRHALEPDGQLTPYPAQVRARYEAWLAA
                     GERAGKRFTPEQRWWLDKIAETIGLNLGIVPGDFDNDGEFLARGGRWGVTEALGPEWR
                     AVLEEMNRELTV"
     gene            3791..4435
                     /locus_tag="PLC06_12825"
     CDS             3791..4435
                     /locus_tag="PLC06_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91376.1"
                     /translation="MNQYDYRPDYRRNLPHYQPRGAILFVTFRLAGSIPIVVLQRLKA
                     EAEAREQRIRQAGDGPEQQRLLYEERKRQFGRWDDVLDEAKEGPHWLREPRIAAIVYD
                     ALCYRDGRVYDLDVFCIMPNHVHAVFAPLVKEDGTYHPLQTIMHSLKRYTAGQGNDAL
                     GREGAFWQHESYDHVVRNPDEWGRIVNYVLHNPVKAGLADTWEEWPWTYARWRV"
     gene            4471..>4701
                     /locus_tag="PLC06_12830"
     CDS             4471..>4701
                     /locus_tag="PLC06_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91377.1"
                     /translation="MTNGNGKWPLPEGWVWTEIGNVLSIRNGYAFNSKDYSDSGTLLV
                     RQTNLSGDKVDINKAVFLPEHYQERYKAFVVQK"
BASE COUNT          873 a         1534 c         1516 g          778 t
ORIGIN      
        1 ttcgcccaca ccggccatcg cccctggccg ctgccgcgcg gccgctggac atggcggcag
       61 tcgtggcgcg atctgctgtt cgcccactgg ccggtgccca cggaggcgat tcggtcgctg
      121 gtgcccgccg gactggccgt ccaggagttc gacggcacgt cctggatcgc cgccgtcccg
      181 tttcgcatgg cgggggtgat gcggcggccg ctgcccgacc tgccgtgggt gtcggccttc
      241 ccgcagctga acgtgcgcgt ctatgtggag gcggagggca agccgggggt gttcttcctc
      301 agcatcgacg ccgccaaccc gctggtcgcg gccatcgcgc ggacgctgtt ccatgtgccc
      361 tacttttggg cggatatgcg tctggagcga cgcggggggt ggattcagta tgagtcggtg
      421 cggcgagggg gaaagggaga aggggagaag gggggaaggg gagaggggga ggcggtgttt
      481 cgcggccgct accggccgac atccgaggtc tacgcggccg cgccggggac gctggagcat
      541 ttcctgacgg agcgctactg cctctatgcc gtgtcggccg atggggtggt gcgccggacg
      601 gaagtccacc acgcgccgtg gccgctgcag cgggcggagg cggagatcga gaccaacacg
      661 atgacggccg cgggcggcgt ggcggtggac gacccgccgg cgctgctcca tttttcgcgg
      721 cggattgatg tggtggtgtg ggggagtgag ggggtgtaga gcgatttgcc aaatcgctcg
      781 caacttggca aatcgctcgc aacttggcaa atcgcgagcg atttaccaaa tcgctctaca
      841 ttcgccattt cgtaagatgt ccggcaagat cgttttgtta taatctcttc aagtgccatg
      901 acacccgaag tattagctcg ccaacagatc gaccagctgc tccagcatgc cggctgggag
      961 gtgcaggatc gcgcccggat gagtctgttc ggccgccccg gccgtggtgt ggccctgcgc
     1021 gaggcggtca tgaccaccgg cgaggccgat tacctgctgc tggtggacgg ccgcgcgctc
     1081 ggcgtcatcg aggccaaacg caccggccac acgcttagcc acgtcgaaga gcagtcgggg
     1141 cgctatgccg tcggtctgcc gccgggtgtc aaaccctggc tgccgggccg gccgctgccc
     1201 ttccgctacg aatccaccgg cgtcgagaca ttcttcacca acggtctcga cccggcgccc
     1261 cgcagccgtc ctgtctttgc cttccaccgc ccggcaacgc tggccgaatg ggccgacgag
     1321 ccgctgacac tgcgcgcccg gctgcgccga atgccgccgc tcatcaccga cgcactctgg
     1381 ccgccgcaga tagccgccat cgccaacctg gagcgctcat tgatcgagga ccggccgcgc
     1441 gccctgatcc agatggccac cggcagcggc aagaccttca ccgccgtcaa cttcgtctat
     1501 cgcctgattc gccatgccgg cgcccgccgg gtcctgtttc tggtcgaccg taacaacctc
     1561 ggtcggcagg cctacaccga gttcaccaac ttcatcacgc ccgacgacgg ccgcaagttc
     1621 gaagacctct ataacatcca gcacctgacc ggcaagatca tcgatcccgt caatcgggtg
     1681 gtcatcagca ccatccagcg gctctacgcc atgctgcgcg atgaggagct ggaggaagat
     1741 gccgacctcg gctcgaccta cgaactggag gagcgcggcc aactggagag cgcgtcgcgc
     1801 acggtcagct tcaccggccg gctgcccatc gagcagttcg atttcatcat cgtcgacgag
     1861 tgccaccgct ccatctacac cgtctggcgg caggtgctgg agtatttcga cgcctccctg
     1921 atcgggctga cggccacgcc cggcaagcag accatcggct tcttccgcca gaacatggtc
     1981 tccgaatata cccacgagat ggccgtggcc gacggcatca acgtcgccgg ggacgtctat
     2041 cgcatccgca cccgcatcgg cgacgagggc agcaccgtcg aggccggcta ctacgtcgac
     2101 cggctggaca agctgacgcg ggcgcgccgg gcggaattgc tggatgaaga cctgacctac
     2161 accgccgacc aactcgaccg caacgtcgcc agcgagagcc agatccgcac cgtcatccgc
     2221 gccttccgcg acgccctgtt caccgaactg taccccgacc gaaccgacca ggtggtgccc
     2281 aaaacgctca tcttcgccaa ggacgacaac cacgccgaga ccatcgtccg cgtggcgcgc
     2341 gaggagttcg ccaagggcaa cgacttctgc cagaagatca cctatcgagc cgagcgcaac
     2401 ccggaagaca tcctcaagga cttccgcaac agctataacc cgcgcatcgc cgtgaccgtg
     2461 gatatgatcg ccaccggcac cgacgtgcgt gccatcgaga ttttgttgtt catgcgccgg
     2521 gtgcgctcgc gcggctattt cgagcagatg cgcggccgcg gcacgcgcat catcagcagc
     2581 gacgacctgc aggccgtcac ccgcgacgcc cgccacaaaa cccatttcat cctgatcgac
     2641 gccgtggggc tggtggaggc gggcatggcc gagacgacgc gcacgctgga gcgcaagcgg
     2701 tcggtgccgt tcaaacaact gctggagggc atcgcctttg gggccgacga cgaggatacc
     2761 gtgtcgtcat tggcggggcg gctggcccgg ctgcaacacg aattgacggc cgccgacgaa
     2821 agccgcatcc gcgaggcgac cggcggccgc gacatggccg ggctgatcgg gctgctcttc
     2881 gacgcgctcg accccgaccg gcagatcgcc caggcgcggg cggcaggcta tccggccccc
     2941 ggcgaggccg agatcgaggc cgcggcggcc gaattgcgcg gcatcgctgt ggcccccttc
     3001 cgcgacagcc ccgacctgcg cgaggcgctc ctcaccgccc aggggcgcaa cgagatcatc
     3061 atcgacaccg tcagcgccga cgaggtgacc gtcaggggct tcgacgccgc ggccaccgac
     3121 cggctacgca agacagtcga tgacttccgg caattcatcg aggccaacaa ggatgagatc
     3181 gccgcgctgc agatcctcta caatgaaccc tacggccgcg cccggccgac gcgcgcgcag
     3241 ctgaaggagc tggccgcggc gctgcaacgg ccgccccatt tgtggacgga agccgcgctg
     3301 tggaatgcct atgccgctgt ggagcgcgac cgggtgcgcg gggcgggcga gacgcgggtg
     3361 ctgaccgatc tggtggccct ggtgcgccac gcgctggagc cggacggaca actaaccccc
     3421 tacccggccc aggtgcgggc gcgctatgag gcctggctgg cggccgggga gcgggcgggc
     3481 aagcgcttca cgcccgagca gcgctggtgg ctggacaaga tcgccgagac gatcggcctc
     3541 aatctgggca tcgtgccggg cgacttcgac aacgacggcg aatttctggc ccgcggcggt
     3601 cgctgggggg tcacggaagc cctcggcccc gagtggcggg cggtgctgga ggagatgaac
     3661 cgggaactaa ccgtgtagcg cgatttgcca aatcccggca gtgttgagcg atttgccaaa
     3721 tcgctttact atgctgcgat ttgtcaaatc gctctactgt taggcgattt gccaaatcgc
     3781 tcaacaagat atgaatcaat acgattaccg gcccgattac cgacgcaacc tcccccacta
     3841 tcaaccacgg ggcgcgatcc tgttcgtcac cttccggctc gccggctcta ttcccatcgt
     3901 tgtgctccag cggctgaagg ccgaggccga ggcccgagaa caacgaatcc gtcaggccgg
     3961 cgacggcccc gaacagcaac gactgctcta tgaagaacgc aaacgccaat tcggccgatg
     4021 ggacgacgtc cttgatgagg ccaaagaagg cccgcactgg ctgcgcgaac cacgcatcgc
     4081 ggcgattgtc tacgacgcgc tgtgttaccg cgacggccgc gtctacgacc tggacgtttt
     4141 ctgcatcatg cccaatcatg tccatgccgt cttcgcgccc ctggtcaagg aggatggaac
     4201 ctatcacccg ttgcagacca tcatgcattc gctcaaacga tacaccgcgg ggcagggcaa
     4261 tgacgcgctt ggccgggagg gcgctttctg gcagcatgag tcctacgatc acgtcgtgcg
     4321 caacccggat gaatgggggc ggattgtgaa ctacgtcctt cataatccgg tgaaagccgg
     4381 gttggcggat acatgggaag agtggccttg gacttatgcg cggtggcgcg tgtagcgcga
     4441 tttggcaaat cgcgccacaa gggaggcatg atgacaaacg gaaacggaaa atggccgctg
     4501 ccggaggggt gggtgtggac tgagatcggt aacgtcctat ccatccgcaa cggttatgca
     4561 ttcaatagca aggattattc tgactcggga accctactag tacggcaaac caatctatct
     4621 ggcgacaaag tagacatcaa caaagctgtt ttcttacctg aacactatca agagcgatac
     4681 aaggcttttg ttgtgcaaaa g
//
LOCUS       DAOMQG010000275         4659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7727_length_4659_cov_9.266290, whole genome shotgun sequence.
ACCESSION   DAOMQG010000275 DAOMQG010000000
VERSION     DAOMQG010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7727_length_4659_cov_9.266290"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLC06_12835"
     CDS             <1..260
                     /locus_tag="PLC06_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRO91378.1"
                     /translation="LADLRHHGIAAAGTVARGGTSLPDVIWPRAGSAILMGNEAEGLP
                     PEIEAALDFSITIPMPGDVDSYSVNAAAAIILYTAARCRET"
     gene            436..828
                     /locus_tag="PLC06_12840"
     CDS             436..828
                     /locus_tag="PLC06_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRO91379.1"
                     /translation="MEINSWHETGQVLVTILQLKGDLTAEEPLTSKAQAVFQDGARDI
                     VLDIGDVPYISSAGLRAIHVIYMMLRSADPEDEGTAVRGIARGTYKSPHLKLVNPSRN
                     GLKALTTSGYDIFLDIHGNIPEAVASFK"
     gene            825..1259
                     /locus_tag="PLC06_12845"
     CDS             825..1259
                     /locus_tag="PLC06_12845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91380.1"
                     /translation="MKSLTLRYSTATIRDLNDIREFLESALLSLGSDSDTAGDLVLAV
                     NEAVTNTLLHGYRDRPGPVAVCAEADGHDLLVRLLDETPLFDPTAVPPPDINLPLEER
                     PLGGLGVHMMRQLTDALLYRVTKDGHNELIFVKRGVLAAPRT"
     gene            1274..2431
                     /locus_tag="PLC06_12850"
     CDS             1274..2431
                     /locus_tag="PLC06_12850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRO91381.1"
                     /translation="MVNAGNPPAIDALQLTPDERALFFSEEGRIVLPSQTVIMREGDP
                     GDEMFVVLEGELVISRLGKQIDRLGPGMILGEMAMVDDQPRSATATTATPCSLIRLDR
                     NGFRDLVGRSPEFALRVMNIMSVRTRRLIEEEVQRQRMEEELAIGRRIQLSLLPSACP
                     LVPGYEFAAGYRAAREVGGDLYDFIIQPDKPNLAHIVIADVTGKGVPAALYMAVSRTL
                     LHTYALDGNPPSQALARVNQFIREDKTSPLFLSAFYAVLDTNIHCLTYANAGHNAPIW
                     LQPSTGDMEYLNARGIVLGAFDAFIPEERLCFLQPGDYVIFYTDGITEARNPQGDFFD
                     EEGLEAVVAAREWDSANELLTAIITAVEEFSAGAPQADDYTVIVLRRKPLP"
     gene            2581..2946
                     /locus_tag="PLC06_12855"
     CDS             2581..2946
                     /locus_tag="PLC06_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRO91382.1"
                     /translation="MHELSVTESILEIVLRHGRNAGATTVTDLFLVVGELSTIVDESV
                     QFYWDIISEGTPAAGSMLHFRRVPGLMGCRACGHTYSPREDLPCPACGSTDIDILAGE
                     EFYLEAIEIAVGDMEKQPI"
     gene            2943..3608
                     /gene="hypB"
                     /locus_tag="PLC06_12860"
     CDS             2943..3608
                     /gene="hypB"
                     /locus_tag="PLC06_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRO91383.1"
                     /translation="MITERVPVVEKILGANDRLAADNRARLDAAGVYSLNFLASPGAG
                     KTSLVEQTVGRLAGRLRLAVVDGDIATSLDADRAAAAGAKAVQINTGGECHLDAVMLQ
                     GALEVLDLPAVDLLIVENVGNLVCPANFALGTHKSVLVASVPEGDDKPYKYPGTYRGV
                     DALVINKIDLLPYVDFDMEYFRRGAAALNPGLATFPMSCRTGEGLDTWIEWVIAQVEA
                     NRG"
     gene            3611..>4659
                     /locus_tag="PLC06_12865"
     CDS             3611..>4659
                     /locus_tag="PLC06_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRO91384.1"
                     /translation="MTAVNDLNGLRIHASGVVQGVGFRPFVYGLAQRHDLKGWVRNSS
                     SGVDIEVDGPAAVLTNFTHDLLAELPPLARIDDWQITERPPNGCTDFTILESKPELGA
                     FQPISPDMTICPDCLHELFDPADRRYRYPFINCTNCGPRYTIIRDIPYDRPLTTMADF
                     PLCADCAAEYRDPLDRRFHAQPVACPVCGPRLWLETPATKDFEPTSAIPATRQAFCEG
                     KIVAVKGLGGFHLACDATNPAAVRELRRRKLRVDKPFAVMIPDIETVRRHCCTSAAEE
                     ELLLSRARPIVLLRRRPESAIARETAPGQNLIGVMLPYTPLHYLLLEPAAGFPEALVM
                     TSGNLSEEPIAYTND"
BASE COUNT          909 a         1511 c         1355 g          884 t
ORIGIN      
        1 tgctcgccga cttgcgccat cacggcatcg ccgccgcggg aacagtcgcc agagggggta
       61 cgtccttacc cgatgtgata tggccgcgcg caggttcagc gatccttatg ggcaacgaag
      121 cggaaggcct gccgcccgag atagaagcgg cgctcgattt tagcattacg atacccatgc
      181 ccggcgacgt ggattcgtat tcggtcaacg ctgcggcggc tatcatcctt tacacggctg
      241 cccgatgtcg cgaaacataa gcagccctcc ggctcacatt cgcaaccggc tgaaagagcc
      301 aatttccgaa aagcctgacc gttgaccgtc gcccattcgc taggtactat ataagcgacc
      361 ctgacggccg cagcccgtcc cggcgcgagc ctcaaaacat aaatccacaa tcgcagcaag
      421 cgggaggcaa ttcacatgga aatcaacagc tggcatgaaa ccgggcaggt tctggtgact
      481 attctacagt tgaagggcga tttaacagcg gaggaacccc tgacaagcaa ggcccaggcc
      541 gtctttcagg acggcgcgcg cgacatcgtc ctggacatag gggatgtgcc ctatatcagc
      601 agcgccggat tgcgggctat tcatgtgatt tatatgatgc tgcgcagcgc cgaccccgaa
      661 gacgaaggga cggccgtccg cggcatcgcc cgcggcacct ataaatcgcc ccatctcaag
      721 ctggtcaatc catctcgaaa cggattaaaa gccttgacaa cttcggggta cgacatcttc
      781 ctggacatcc acggtaatat tcctgaagcg gtcgcctcat tcaaatgaaa tcgctgacgt
      841 tgcgttattc aacggcgacg atccgcgatc tcaacgatat ccgcgagttc ctggaaagcg
      901 cgctcctgtc cctcggcagc gacagcgata ccgccggcga tttggtgctg gcggtgaacg
      961 aggccgtcac caacaccctg ctccacgggt accgggatcg gcccggcccg gtggccgtct
     1021 gcgccgaagc ggatggccac gatctcctgg tgcggctgct ggatgagacc cccttgttcg
     1081 atcccacggc cgttcccccg cccgacatca atttgccgct tgaggaacga ccgctgggcg
     1141 gcctgggcgt tcatatgatg cggcagctga ccgacgcact tctctatcgc gtgaccaaag
     1201 atggccacaa cgaactgatt ttcgtcaaac ggggagttct cgccgctccc cggacctgat
     1261 tgacagacgc cctatggtta acgccggcaa tccccctgct attgatgcac tgcaactaac
     1321 cccggacgaa cgcgcgctct tcttctcgga ggagggccgc attgtcttgc cgtcgcagac
     1381 ggttatcatg cgtgaaggcg atcccggcga tgagatgttc gtggtgctgg agggggaact
     1441 ggtcatcagt cgtctgggca aacaaattga tcgcctgggg ccaggcatga tcctgggcga
     1501 gatggcaatg gtcgacgatc agccgcggag cgccacggcc acgacggcca ccccgtgcag
     1561 cctgatccgc ctcgatcgaa acggctttcg cgatctggtc ggccgttcgc cagagttcgc
     1621 cctgcgggtg atgaatatca tgtccgtgcg gacgcggcga ctcatcgaag aggaagtgca
     1681 gcggcaacga atggaagagg aactggccat cggccgccgt atccagttga gcctgctgcc
     1741 gtccgcttgc ccgctggtgc cgggctatga gtttgccgcg gggtatcgcg ccgcgcgtga
     1801 ggttggaggc gacctctacg acttcatcat ccagcccgac aagcccaatc tggcccacat
     1861 cgtcatcgcc gacgtgaccg gcaagggcgt gccggccgcg ctctacatgg ccgtttcgcg
     1921 caccctgctg cacacctatg cgctggatgg caacccgccg tcgcaggcac ttgctcgcgt
     1981 taatcagttc attcgcgagg acaagaccag cccgctgttc ctgagtgcct tttatgccgt
     2041 gctcgatacc aatatccact gcctcaccta tgccaacgca ggccacaacg cgcccatctg
     2101 gctccagccc agcaccggcg acatggagta tctgaacgcc cgcggcatcg tcctgggcgc
     2161 gtttgacgcg tttatccccg aagagcgcct gtgctttctc cagccgggcg actatgtcat
     2221 cttctacacc gacggcatca ccgaggcccg caatccgcag ggcgatttct tcgatgagga
     2281 aggactggaa gccgtcgtcg ccgcgcggga atgggacagc gccaacgaat tgctgacggc
     2341 gatcatcacc gctgtcgaag aattctccgc cggcgcgcca caggccgacg actacaccgt
     2401 gattgttctg cgtcgcaagc ccctacccta atctccatca atctcgttcc ctccatcttg
     2461 tgacattgat aggccgaacg agtgacgttt gtcactcata aatcgacctg ccaaaccgta
     2521 tagttatcgt tgaagagtcc atcgttcgac ctgtcattta tcggctcaac ggcaggacat
     2581 atgcacgaat tatccgtcac cgagagcata ctggagatcg tcttgcgtca tggtcgcaac
     2641 gcaggcgcga cgaccgttac cgatctcttt ctggttgtgg gcgaactctc cacgatcgtg
     2701 gatgagtcgg tgcagttcta ctgggacatc atcagcgaag gaacgccagc cgccgggtcg
     2761 atgctccact tccggcgcgt gcccggcctg atgggttgcc gcgcctgtgg ccacacctac
     2821 agcccgcgcg aagacctgcc ctgtccggca tgcggcagca ccgacatcga tatcctcgcc
     2881 ggtgaagagt tctatctgga agccatcgag atagccgtcg gcgatatgga aaagcaacca
     2941 atatgatcac cgaacgcgta cccgttgtgg aaaagattct cggcgccaac gaccggttgg
     3001 cggccgataa ccgcgcccgg ctcgacgccg ccggcgtcta tagcctcaac ttcctggctt
     3061 cgcccggcgc gggcaagacc agcctggtgg agcaaaccgt cggccgtctg gcgggcaggc
     3121 tgcgcctggc cgtggtcgac ggcgacatcg ccaccagcct ggatgccgac cgggccgcgg
     3181 ccgccggcgc aaaagccgtg cagatcaaca ccggcggcga gtgccatctc gacgcggtga
     3241 tgctgcaggg cgcgctggag gtgctggacc tgccggccgt tgacctgctc attgtcgaga
     3301 acgtgggcaa tctggtctgc ccggccaact tcgcactggg cacacacaag agcgtcctcg
     3361 tggcctcagt tcccgaggga gacgacaagc cctataaata ccccggcacc taccgcggcg
     3421 tcgacgcgct ggtgatcaac aagatagacc tattgcccta tgtcgatttc gacatggaat
     3481 acttccggcg tggcgcggcc gccctgaatc cggggctggc gaccttcccg atgtcctgcc
     3541 gcacgggcga agggctggat acctggatcg agtgggtcat cgcccaggtg gaagcaaatc
     3601 gcggatgaac atgaccgccg tcaacgacct gaatggcctg cggattcacg catccggcgt
     3661 cgtccagggc gtgggcttcc ggccgtttgt ctatggtctg gcccagcgac acgatctgaa
     3721 aggttgggtc cgcaactcct cttcgggcgt cgatattgaa gttgacggcc cggcggccgt
     3781 cctgaccaat ttcacccatg atctgctcgc ggaattaccg ccgctggcgc gcattgacga
     3841 ttggcaaata accgagcggc cgcccaacgg ttgcacggat ttcacgatat tggagtcgaa
     3901 gccggagttg ggggctttcc agcccatctc ccccgacatg acgatttgcc ccgactgcct
     3961 gcacgaattg ttcgacccgg ccgaccggcg ctatcgctac ccgttcatca attgcaccaa
     4021 ttgcggccca cgctacacca tcatccgcga tatcccctat gaccggccgt tgacaaccat
     4081 ggccgacttt ccgctctgcg ccgactgcgc ggccgaatat cgcgatccgc tcgaccgccg
     4141 ctttcatgcc cagccggtgg cctgtcccgt ttgcggcccg cgcctgtggc tggagactcc
     4201 tgccaccaag gattttgaac ccacctccgc cataccggcc acacgacagg ccttctgcga
     4261 gggcaagatc gtggccgtga aaggcctggg cggattccat ctggcctgcg acgcgacaaa
     4321 cccggcggcc gtgcgcgaat tgcgtcgtcg caagctgcgc gtggacaagc cgtttgccgt
     4381 gatgattccc gatatcgaaa cagtccgccg ccactgctgt acaagtgcgg ccgaggaaga
     4441 gctgctcctc tcgcgagcgc ggccgatcgt cctgctgcgt cgccgtcccg aatcagccat
     4501 cgcccgcgaa accgcgccgg gccagaacct gatcggcgtg atgctgccct atacgccact
     4561 tcattatttg ctgctggagc ctgcggcggg cttccccgaa gcgctggtga tgaccagcgg
     4621 caacctgagc gaggaaccga tcgcctatac caacgacga
//
LOCUS       DAOMQG010000276         4648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7752_length_4648_cov_10.818637, whole genome shotgun sequence.
ACCESSION   DAOMQG010000276 DAOMQG010000000
VERSION     DAOMQG010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7752_length_4648_cov_10.818637"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /gene="tsaE"
                     /locus_tag="PLC06_12870"
     CDS             complement(<1..476)
                     /gene="tsaE"
                     /locus_tag="PLC06_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRO91385.1"
                     /translation="MAILDDRTLDFVSTSVAQTERLGIRLGELLEPNDVVCLSGELGA
                     GKTALARGVGRGWGSPARVTSPTFVLVNEYPRSSDGCILYHIDCYRLERTADIASVGL
                     EDILDSKAAALIEWPEHIADLLPPDRLWITLSYDSPTRRRLRLSAMGPRSSVLLVD"
     gene            666..1394
                     /locus_tag="PLC06_12875"
     CDS             666..1394
                     /locus_tag="PLC06_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRO91386.1"
                     /translation="MRTLVLSDIHANLVALEAVLTAAGTWDRVWFLGDLVGYGPNPNE
                     CVERLRELEPLALSGNHDWAVLNKLDTEEFNDDARRAVNWTRRELTEENMDYLRGLPP
                     LHVAPPFTMAHASPRHPVWEYILDLQTALDNFDHFETPICLVGHSHIPALFLLDDTAA
                     ELNFYLLGDAETVDLSQGRIILNPGSVGQPRDGDPRASFAILDDEAQTWTLHRVEYDI
                     AETQRRMHAHKMPSRLIERLEFGM"
     gene            1562..2503
                     /locus_tag="PLC06_12880"
     CDS             1562..2503
                     /locus_tag="PLC06_12880"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRO91387.1"
                     /translation="MKRRILLAVTLFIAALSAVACAGQGARSEQVMTEESAAVYAVPA
                     EAPAADFAVNQSFDAVEAEATGQLPGSQERLIIRTADMSITAADTEATLAQIAAMADS
                     SGGWVVSSNIYQSSDTYKTGYVTIRVPSEGFQSVLDAIAGLAVEVTNLSTSGQDVTEE
                     YVDLSARLSNQEATAARLRQFLEESTNVEDALAVNVELSRVEGEIEALKGRIQFLGQS
                     SAFSSITVNVSPDALAQPIQVGGWRPSGVAKDAIEALITMLQVLANAAIWFVLFAVPI
                     LIVIAIPFVLLIWVIKRLRRRESRVAARETGDTDTGA"
     gene            2627..2920
                     /locus_tag="PLC06_12885"
                     /pseudo
     CDS             2627..2920
                     /locus_tag="PLC06_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
     gene            2948..3346
                     /locus_tag="PLC06_12890"
     CDS             2948..3346
                     /locus_tag="PLC06_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO91388.1"
                     /translation="MPPGALIPELAYDDVEVAATWLCEAFGFRERLSIGNHRRQLTCQ
                     GESIVATAGGDAAAAGANHSIMMRVADVDAHYARAKAAGAKILGAPESYFYGERQYSA
                     EDIGGHVWTFSQSVADVAPEEWGGQLIDPS"
     gene            3552..3773
                     /locus_tag="PLC06_12895"
     CDS             3552..3773
                     /locus_tag="PLC06_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO91389.1"
                     /translation="MKQWETLEAVERDPNKVSGVWVFRGTRVPVAALFENLRDGATIE
                     EFLEWFPGVERAQVEAILDHEIEMLAVAV"
     gene            complement(4112..>4648)
                     /locus_tag="PLC06_12900"
     CDS             complement(4112..>4648)
                     /locus_tag="PLC06_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91390.1"
                     /translation="ETPVAVLSGSFNPLHAGHTGLAETAAALLGVPVTFEVSAVNVDK
                     PPLETTVLLDRLSQFAGRWPVVAGNAPTFVEKSRLYPGAVFVVGFDTAARIIQPRYYG
                     DSEAGMLAALAEMRDHGNRFLVAGREGDDGHFRELRELEIPAGFAGLFEAIPERLFHS
                     DVSSTEIRAALKAEVDNK"
BASE COUNT          945 a         1421 c         1388 g          894 t
ORIGIN      
        1 aaatccacca gcagaacgct cgaccgcggc cccatggcgc tgaggcgcag gcggcggcgg
       61 gtgggactgt catacgacag ggtgatccac aggcggtcgg gcggcagcag gtcagcgata
      121 tgttcgggcc attcaatgag cgcggccgcc ttgctgtcga gaatatcctc cagaccaacc
      181 gaggcaatgt cggccgtgcg ctccagccga tagcagtcga tgtggtagag gatgcagccg
      241 tcgctggagc gcgggtattc gttcaccagc acgaaggttg ggctggtgac gcgggccggc
      301 gaaccccaac cgcggccgac gccgcgcgcc aacgccgtct tgcccgcacc cagctcgccc
      361 gacaggcaaa cgacgtcgtt cggctccagc agctcgccca ggcgaatgcc caggcgttcc
      421 gtctgcgcca cacttgtgct gacaaaatcc agtgttcggt cgtcgagaat cgccatacgt
      481 tcgtaacgtt gatcagcccc gaaatcgctc gttgagtagc gcagccggac atcctgcctg
      541 catgagcctg attgaacgtc tattatactt tcatggtatg atatacgcat ctgaacgctt
      601 atgccgttcg cgaccatcta tggataactg ccggctatct atcattcact acccgctctc
      661 ccctcatgcg tacactcgtt ctttctgata ttcacgccaa tctcgtcgcc ctggaggcgg
      721 tgctgaccgc tgccggcacc tgggatcgcg tctggtttct gggcgatctg gtcggctatg
      781 gccccaatcc taacgaatgc gttgagcggc tgcgcgagct ggaaccgctg gcgctgtcgg
      841 gcaatcacga ctgggcggtg ctgaacaagc tcgacaccga ggagttcaac gacgacgcgc
      901 ggcgtgcggt gaactggacg cgccgggaat tgaccgagga aaacatggat tatctgagag
      961 gcttaccgcc gctccatgtg gccccaccct ttacgatggc ccacgccagc ccgcgccacc
     1021 ccgtatggga atacatcctc gatctgcaaa cggcgctgga caacttcgac catttcgaga
     1081 cgcccatctg cctggtgggc cattcccaca tcccggccct gtttctgctg gacgacacgg
     1141 cggccgagct gaatttctac ctgctcggcg acgccgagac ggttgacctg agccaggggc
     1201 ggatcatcct gaaccccggc agcgtgggac agccacgtga cggcgatccg cgcgcgtctt
     1261 tcgccatcct cgacgacgag gcgcagacgt ggacgctcca ccgcgtggag tacgacatcg
     1321 ccgagactca acgccgcatg cacgctcaca agatgccttc acgtttgatc gagcggctgg
     1381 aatttggcat gtagcgcaag cattcctgct tgcgcctgta tcgggtcagg taaggcagtt
     1441 ttgctattgt tcggcgctca gtcaggaatg gctgagctac gagggaactt gcgactatga
     1501 tccgccgtct ttattccaga ggcaacaacc aatgaatttg ccgaacaagg aagggtcaat
     1561 catgaaacgc cgaattttat tagcagtcac tttgttcatt gccgccctga gcgccgtggc
     1621 gtgtgccggc cagggtgctc gatcggagca ggttatgacc gaagagtcgg ccgcggtcta
     1681 tgctgttccc gccgaagcgc cggcggccga tttcgcggtc aatcagagct tcgatgctgt
     1741 cgaagcagag gcaaccggcc aactccccgg cagccaggag cggctcatta ttcgcacggc
     1801 cgatatgtcc atcaccgccg ccgacaccga agcaacgctg gcccagatcg ccgccatggc
     1861 cgacagcagc ggcggctggg tagtcagcag caatatctat cagtcaagcg acacctacaa
     1921 aaccggctac gtcacgatcc gcgtcccgtc cgagggattc cagagcgtgc tcgacgccat
     1981 cgccgggctg gccgtggaag tgaccaacct cagcacttcg ggtcaggatg tgaccgagga
     2041 gtacgtcgat ctgagcgccc gactcagcaa tcaggaagcg acggccgcac ggctgcgcca
     2101 gtttctggaa gaatccacca acgtcgagga tgcgctggcg gtcaacgtgg aattgagccg
     2161 cgtcgagggc gagatcgagg cgctgaaggg gcgcatccaa tttctggggc agtcgtcggc
     2221 cttctcgtcc atcaccgtca atgtatcgcc cgacgcgctg gcccagccga ttcaggtcgg
     2281 cggctggcgg cccagcggcg tggccaagga tgccatcgag gcgctgatca ccatgctgca
     2341 agtgctggcc aatgccgcca tctggttcgt gctcttcgcc gtgcccatcc tgatcgtcat
     2401 cgccatcccg tttgtgctgc tcatctgggt catcaagcgg ctgcggcggc gcgagtcgcg
     2461 ggttgccgca agagaaaccg gcgacacaga taccggcgca taacgaatca cacaaaaggc
     2521 ttatcactcg ttagcgaagt aagcgttggc aggtgggcgg aaaggtttcg tttaaacgag
     2581 gctttgtcgc ccacctgtca tgtttggtaa gtcaaggaga ataaaaatgt acggattaat
     2641 cggcaagatg acggccgcaa ccgggcagcg cgactcgctg atcgccattc tgctcgacgg
     2701 catcgccgga atgccgggct gcctgagcta tatcgtggcc aaagacccga ccgacgccga
     2761 cgctatctgg atcaccgaag tctgggacag cgccgagagc catcgcgcgt ctctgtcgct
     2821 gcccgccgtg cgagaagcta tcatccgcgc tcggccgctg atcgcagggt tcgatagctc
     2881 catcccgacg gagccggtcg gaggcgccgg actggtcgct ccgacagcag acatcgccaa
     2941 tcgctccatg ccgcccggcg cgctcatccc tgaactggct tacgacgacg tggaggtggc
     3001 cgctacctgg ctgtgcgaag cgtttggttt tcgcgagcgg ctgagcatcg gcaaccaccg
     3061 ccggcagtta acttgccagg gagaatcaat cgtcgccaca gccggcggag atgcggcggc
     3121 ggccggcgcg aaccactcga tcatgatgcg cgttgccgat gtggatgccc actacgcccg
     3181 cgccaaggcg gcaggcgcga aaatcctggg cgcgcctgaa tcctacttct acggcgagcg
     3241 gcagtactcg gccgaggaca tcggcggcca cgtctggaca ttcagccagt cggtggccga
     3301 cgttgcgccg gaggaatggg gcgggcagtt gatcgatccg tcataattgg gcgtcgatga
     3361 ctggcatggg cacgccgatc gccgaacccc aacgcctcca catgcgggag ttcgccgcct
     3421 ccgatctggc ggccatgacc catatcttcg gcgacgccga ggtgatacgt ttaggcgatg
     3481 gcccacaatc ggtcggatct ggtataattc agccatctgc tgattcatct tttcttccag
     3541 aggagaacaa aatgaaacaa tgggaaacgc tggaagctgt tgaacgcgat ccaaacaaag
     3601 tcagtggggt ttgggtattt cgtggaacca gggtaccggt tgcggccctt ttcgagaatc
     3661 tccgggatgg agcaacgatt gaggagttcc tggaatggtt tccaggtgta gagcgagcgc
     3721 aagttgaggc gatactcgat catgagatcg agatgttggc ggtcgccgtc taaacgtgaa
     3781 gatccttttt gatcagggag tgccgctccc tctacggcgc tatttggccg aacatgacgt
     3841 gtcaactgcc tataggcagg gttggtctac tctatcaaat ggcgatttgc taaagagggc
     3901 tgaagatgct ggttatcagc tatttatgac caccgatcag aatttgagtt atcagcaaaa
     3961 cctgctcgac cgacgaatcg cgatcgttgt actcctctcg acctcgtggc ccaaaatcca
     4021 cttgcgcatt gctgagatag ctgatgtcat taacgccatt acatcgaacg aatatataga
     4081 agtttcgata taaataagcg tccagcattt cctatttatt gtcgacctca gccttcaacg
     4141 ccgcccgaat ctccgtcgat gacacatcgg agtgaaacaa cctctcagga atagcctcaa
     4201 ataacccggc aaaccccgcc ggaatctcca attcgcgtag ttctctgaaa tgaccgtcgt
     4261 ccccttcccg cccggcgacg aggaagcggt tgccgtggtc gcgcatctcg gccagcgcgg
     4321 ccagcatgcc cgcctcgctg tcgccgtagt agcgcggctg aatgatccgg gcggccgtat
     4381 cgaagcccac cacaaacacc gctcctggat aaagccgcga cttctcgacg aatgtcggcg
     4441 cgttgccggc cacaaccggc caacgcccgg cgaactggct cagccggtcg agcaggacgg
     4501 tcgtctccag cggaggtttg tcgacgttga cggccgatac ttcgaaggtc accggcacac
     4561 ccagcagcgc cgcggccgtc tcggccagcc cggtgtggcc ggcgtgcagc gggttgaacg
     4621 agccggacaa cacggccacc ggcgtctc
//
LOCUS       DAOMQG010000277         4643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7755_length_4643_cov_9.052964, whole genome shotgun sequence.
ACCESSION   DAOMQG010000277 DAOMQG010000000
VERSION     DAOMQG010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7755_length_4643_cov_9.052964"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PLC06_12905"
     CDS             <1..131
                     /locus_tag="PLC06_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRO91391.1"
                     /translation="IEPREYLSVTLSFDHDIVDGAPAARFGGRLRGLIEGGDGLKL"
     gene            complement(219..1061)
                     /locus_tag="PLC06_12910"
     CDS             complement(219..1061)
                     /locus_tag="PLC06_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO91392.1"
                     /translation="MTYRSIFRPDLFAGQTCIVTGGGTGIGRAVAHELASLGAHVVLA
                     ARTLENLERVTAEIREAGGAASHFVCNIREEEQVAALFDFVLAERGGFHGLVNNAGGQ
                     FISPAEMISLKGWRAVVETNLTGTFLMCREAFNRHMGAHGGAIVNMLIEMWRGFPGMA
                     HSAAARAGIDNLTKTLAVEWARAGVRVNAVAPGLISSSGLERYPEAVRPMIAQNIRDI
                     PMKRMGTEAEVSAAIVFLLSPAAAFISGESIKIDGAGSLWRKTWEVGEHGKGAEGYEG
                     FTMS"
     gene            complement(1172..2512)
                     /locus_tag="PLC06_12915"
     CDS             complement(1172..2512)
                     /locus_tag="PLC06_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HRO91393.1"
                     /translation="MTTQEPTQKSPSRWWLIAAFIGGLVIMGLIAALLVNITTRKAEG
                     EQYPLKVVEIADGELDPAVWGKNFPRHYDSFMKTQDSTISTPFGGSIPYSKLERVPAM
                     VRIWAGYAFSIDHNEERGHYYTLTDQLETERIQVVEQPGACANCHSADAPRLIAEMGW
                     EAFNKTPYNELKADLHYGTSCADCHDPQTMELRITRPGLINAMTARGVDLEAATRQEM
                     RTYVCAQCHVEYYFKGDNKELTFPWSQGLSIDDIEGHYDTYGFKDWTHKETNAPMIKI
                     QHPEFEMFSSGLHYKSGVACADCHMPYVREGAVKISDHWLRSPMVNINNACQTCHRQD
                     EAVLLERINTIQNRTAGLLRTTEESLLDAIDAIVAAQNAGATDAQLENARYLHRRASL
                     RWDFVSSENSTGFHSPQESARILADAINYARQAQLEAERLGGTTQTTSTANSTP"
     gene            complement(2528..3031)
                     /gene="nrfH"
                     /locus_tag="PLC06_12920"
     CDS             complement(2528..3031)
                     /gene="nrfH"
                     /locus_tag="PLC06_12920"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HRO91394.1"
                     /translation="MSNSPPEGKQGVLHRIGALAPFRTWLMLTAVLGGIVGLGFFTFI
                     YAQGASYLSDDPSSCVNCHVMRDQYDGWNHGSHKAVATCNDCHTPHSSTVAKYTVKAI
                     NGFRHSAAFTLNNFHEPIRITSLNRRVAQSSCLYCHEAMTAYMNHAESDDPTDCLLCH
                     ARVGHAK"
     gene            complement(3195..4259)
                     /locus_tag="PLC06_12925"
     CDS             complement(3195..4259)
                     /locus_tag="PLC06_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRO91395.1"
                     /translation="MTANATPDDTISMRPDEQIDAGRLGDYLAGKLPDGDRPLAVRQF
                     GGGAANLTYLLDFEGHEYVLRRPPLGPVAASSHDMSREYRVLSVLHQAYPYAPRAFLF
                     CDDESIIGAPFFVMERRHGVVVRRTLPDAYRAIPNAARRMSEALVDALAEFHAVDYQT
                     LGLGQLGKPDGFIDRQIEGWYRRWQAAKTADLAEMDTVYTWLKANQPVNTQVSLVHND
                     YKLDNVMLAADDPGRVVAVFDWDMCTLGDPLADLGALLTYWTEPGDPPYLQATAMMPL
                     GVPGFLSRDELVARYAAKSGRAIGDIDFYHALGLFRLTVIVAQIYIRYARGQTQDPRF
                     AAMEPMIPALARRARELATR"
     gene            complement(4256..>4643)
                     /locus_tag="PLC06_12930"
     CDS             complement(4256..>4643)
                     /locus_tag="PLC06_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91396.1"
                     /translation="VRLLGCEPPPAVAPGETLTLNCYWQAVGEMPLAHYTTFAHLIAV
                     DNGLLLAQDDHVLGRERYPLNAWQSGEIIRETYSLAIPADAAAGEYRIALGIYTWPDL
                     IRLAVPDNPDNVAVLAPVYVEREGEP"
BASE COUNT          850 a         1387 c         1512 g          894 t
ORIGIN      
        1 gcatcgagcc gcgcgaatat ctgagcgtca cgctgagctt cgaccatgat attgtcgatg
       61 gcgcgccggc ggcgcggttt ggggggcggt tgagggggtt gattgagggt ggggatgggt
      121 tgaagttgta gcccacgtat accacccccg gtgcctcatc ctgcaccccc aggattggtt
      181 tgattaccca ccttatctcg atgcctacga attcggggtc agctcatagt gaacccctca
      241 tacccctccg cccccttccc atgctccccc acctcccacg tcttgcgcca taacgacccg
      301 gcgccgtcga tcttgatcga ctcgccgctg atgaacgccg ccgcggggga gagcaggaag
      361 acgatggccg cgctcacttc ggcctccgtg cccatgcgtt tcatggggat gtcgcggatg
      421 ttctgcgcga tcatcggccg caccgcctcc gggtagcgct ccagcccgct ggatgagatc
      481 agccccggcg cgacggcgtt gacccgcacc ccggcccgcg cccactccac ggccagcgtc
      541 ttggtcaggt tgtcgatgcc cgcccgcgcc gcggccgagt gggccatgcc cggaaagccg
      601 cgccacatct cgatcagcat gttgacgatg gccccgccgt gggcgcccat gtggcggttg
      661 aacgcctcgc ggcacatcag gaacgtgcca gtcaggttcg tctccaccac cgcccgccag
      721 cctttgagcg agatcatctc cgccgggctg atgaactggc cgccggcgtt gttgaccagc
      781 ccgtggaagc cgccgcgttc ggccaggaca aagtcgaaca gggcagccac ctgctcttcc
      841 tcgcggatgt tgcagacgaa atgggaagcc gcgccgcccg cttcgcggat ttcggccgtg
      901 actcgctcca gattctccag cgttcgcgcc gccagaacga cgtgagcgcc gagcgaggcc
      961 agctcgtggg ccacggcgcg gccgatgccg gtgccgccgc cggtgacgat acaggtttgc
     1021 ccggcgaaca gatccgggcg gaagatggaa cgataggtga tgggcatggc gtaacctccg
     1081 ccggaaataa taacacctga ccggccgtag cgcgatttgg taaatcgcac tgcaaccggt
     1141 caggtgtcag agagatggag aagaaggcgg attagggtgt gctgtttgcg gtcgacgtgg
     1201 tttgcgtcgt gccgcccagc cgttcggctt ccagttgggc ctggcgggcg tagttgatcg
     1261 cgtcggccag aatgcgggcc gattcctgcg ggctgtggaa gccggtggag ttttccgatg
     1321 agacgaagtc ccagcgcagc gaggcgcggc gatggagata gcgagcgttt tccagctgcg
     1381 cgtccgtcgc tcccgcgttt tgcgcagcca caatggcgtc gatggcgtcg agcagcgact
     1441 cttcggtcgt gcgcagcaga cccgccgtgc gattctggat cgtattgatg cgctccagca
     1501 ggacggcctc atcctgccga tggcaggtct ggcaggcgtt gttgatattg accatcgggc
     1561 tgcgcagcca gtggtcggag atcttgaccg cgccctcgcg gacgtagggc atatgacagt
     1621 cggcgcaggc cacgcccgac ttgtaatgca ggccgctgga gaacatctca aattcggggt
     1681 gctggatctt gatcatcggc gcgttcgttt ctttgtgcgt ccagtccttg aatccgtagg
     1741 tgtcataatg accttcgatg tcgtcgatag acaacccctg gctccagggg aaggtcaatt
     1801 ccttgttgtc gcccttgaag tagtattcga catggcattg ggcgcaaaca taggtgcgca
     1861 tctcctgccg ggtggcggct tccagatcca cgccgcgcgc ggtcatggca ttgataagcc
     1921 cggggcgggt gatgcgcagc tccatcgtct gcgggtcgtg gcaatcggcg caggaggtgc
     1981 cgtagtgaag gtcggccttc agctcgttat agggcgtttt gttgaaagcc tcccaaccca
     2041 tctcggcgat caggcgcgga gcgtcggccg agtggcagtt ggcgcatgcg ccgggctgct
     2101 cgacgacctg aatacgctcc gtttccagct ggtcggtcag cgtgtagtaa tggccccgct
     2161 cctcgttgtg gtcgatgctg aaggcgtagc cggcccaaat gcgcaccatc gccggaacac
     2221 gctccagctt gctgtaggga atggagccgc cgaagggcgt gctgatggtt gaatcctggg
     2281 ttttcatgaa ggagtcgtag tgccggggga agttcttccc ccagacggcc ggatcgagtt
     2341 cgccatcggc gatctcgaca accttgagcg gatattgctc cccttcggcc ttgcgagtgg
     2401 tgatattcac cagcaaggcg gcgatcaggc ccatgatgac cagaccgccg ataaaggcgg
     2461 cgatgagcca ccatcgactg ggtgattttt gcgtgggttc ctgagtggtc atcttcttcc
     2521 tcgtgtatta tttggcatgc ccgacccgcg cgtggcagag caggcaatcg gttggatcgt
     2581 cactctcggc atgattcata taagccgtca tggcttcgtg gcagtagaga caactcgatt
     2641 gggccacacg gcggttgagc gaagtaatcc ggatcggttc gtggaaatta ttgagcgtga
     2701 aggcggcgct gtggcggaag ccgttgatcg ccttcacagt gtacttggcg acggtcgagc
     2761 tatggggcgt atggcagtcg ttgcaggtag ccaccgcctt gtgactacca tggttccagc
     2821 catcatattg gtcgcgcatg acgtggcaat tgacgcaaga cgacgggtca tccgacagat
     2881 aggacgctcc ctgggcatag atgaaggtga aaaaacctaa cccgacgatc ccgcccagaa
     2941 ccgccgtcag catcagccag gtacgaaaag gcgccaatgc cccaatgcga tgcaatacgc
     3001 cctgcttccc ctcggggggc gagtttgaca tggcttatcc ctctcctttg gttaaacgta
     3061 ttcacgagtg aacggcttaa tatggggaaa ccgttacctc acgctctccc acggaatacg
     3121 ttaatgatat gttagccgaa tgcgttaacc cggcaagtcg agcaacatga tggattaggc
     3181 agatttagtc aggtttagcg ggttgccaac tcacgggcac gccgcgccag agcgggaatc
     3241 atcggctcca tcgccgcaaa gcgtgggtct tgcgtctgac cacgagcgta gcggatgtag
     3301 atttgggcga cgatgacggt caggcggaaa aggcccagcg cgtgatagaa gtcgatatcc
     3361 ccaatcgcgc ggccgctttt cgcggcatag cgggccacca gttcgtcgcg actcaggaac
     3421 cccggcacgc cgaggggcat catggccgtc gcctgcaagt agggcgggtc gcccggctcc
     3481 gtccaatagg tcagcagcgc gccgaggtcg gccaagggat cgcccagcgt gcacatgtcc
     3541 cagtcgaaca cggccacgac ccggccggga tcatcagcgg ccagcatgac gttatcgagc
     3601 ttgtagtcgt tgtggacgag tgagacctgc gtgttgacgg gttgattggc tttcagccag
     3661 gtgtagacgg tatccatctc ggccagatcg gccgttttgg ccgcctgcca gcgacgatac
     3721 cagccctcga tctgccggtc gatgaagccg tcgggcttgc ccaattgacc caggcccagc
     3781 gtctgataat cgacggcgtg gaactcggcc agcgcatcga ccaacgcctc gctcatgcgc
     3841 cgggcagcgt tgggtatagc acgataagca tcgggcagcg tgcgccggac gaccacgcca
     3901 tgccgccgct ccatgacgaa gaagggcgcg ccgatgatgg actcgtcgtc gcagaacagg
     3961 aaagcgcgtg gggcgtaggg ataggcctga tggagcacgg acaggacacg atactcccgg
     4021 ctcatgtcat gggacgaagc ggcaacgggg ccgagcggcg gccgccgcag cacatattca
     4081 tgcccctcga agtcgagcag ataggtcaga ttggccgcgc caccgccgaa ctgacgcacc
     4141 gccaacggcc gatcgccatc gggcaacttc ccggccagat agtcgcctaa tcgcccagcg
     4201 tcgatctgct cgtcggggcg catggagatg gtgtcgtcag gtgtggcgtt ggcggtcatg
     4261 gttctccttc tcgttcgacg tataccggcg cgagcacggc cacgttgtcg ggattatcgg
     4321 ggacggccag acgaatcagg tcgggccagg tatagatgcc cagcgcgatc cggtattcgc
     4381 cagcggccgc gtcggcgggg atggcgaggg agtaggtctc acggatgatt tcccccgatt
     4441 gccaggcgtt gagcgggtag cgctcccggc cgaggacgtg gtcgtcctgg gcgagcagta
     4501 ggccattgtc gacggcgatc aagtgagcga aagtggtata gtgggcgagc ggcatctcgc
     4561 cgaccgcttg ccaatagcaa ttcaacgtta gcgtctcgcc cggcgcgacg gccggcggcg
     4621 gctcgcagcc cagcagccgg acg
//
LOCUS       DAOMQG010000278         4613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7829_length_4613_cov_4.375603, whole genome shotgun sequence.
ACCESSION   DAOMQG010000278 DAOMQG010000000
VERSION     DAOMQG010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_4613_cov_4.375603"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLC06_12935"
     CDS             <1..401
                     /locus_tag="PLC06_12935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRO91397.1"
                     /translation="AAAQSAVDAARAALARMTLVAPFAGTVAQIDVEVGELVAPGVVV
                     ARLGSAAGWAMETTDLVELDVPAVTEGQAAVVTLDALPNETLRGTVIDIGRVPEIVRG
                     DVVYRVRIALDDYPDLPLRWGMTADVVGER"
     gene            535..1347
                     /locus_tag="PLC06_12940"
     CDS             535..1347
                     /locus_tag="PLC06_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRO91398.1"
                     /translation="MFDIFKNLDPVLQALLASGFTWGVTALGASIVFFTRTVNRKLLD
                     GMMGFAAGVMLAASFWSLLAPAIELAESNGGPAWLPAVVGFSVGGLFLAAADRLIPHL
                     HIGAPLAEAEGIKTQWQRSALLILAITLHNFPEGLAIGVAFGAVAAGLPAATLPAAIA
                     LAIGIGIQNFPEGIAVAMPLRAEGMSRWKSFFYGQASALVEPVAAVLGASIVLVARNA
                     LPYALSFAAGAMVFVVIEELIPESQSGSNTDLATTCAMGGFVVMMLLDVALG"
     gene            1358..2569
                     /locus_tag="PLC06_12945"
     CDS             1358..2569
                     /locus_tag="PLC06_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRO91399.1"
                     /translation="MNQPNAMRPYYTDSTTLDFAAMIESLEDFDGRPAVLLEPGYFYP
                     TSGGQPHDTGQLRRGDEIARVIDVVERESDGVALHILDRPLSPGPVAASIDRDRRLDH
                     MRHHTGQHILSQAFIRVAEAETVGFHMSAQSITIDLDRADLSAAEIDRAEALANEVVW
                     ADGPVSVRFVTLAEAATLPLRKTPPGRDGRLRLIDIEGFDLTACGGTHVARTGEVGLI
                     RIARTERRGGMTRVEFLCAGRALADYRAGQDVLRQLGAALTTGAAELPAAVARLQEEN
                     KALRLDLRRREDALLALEAAQLLAAAEPASAARLVRRVFVGRSPDEVRRLATALVEAG
                     GVVALLGLAGERSHLVFARSADAPGDMGALVKVALAKLGGRGGGSPTLAQGGGPAADE
                     AAVAAVLQPEL"
     gene            2690..3583
                     /locus_tag="PLC06_12950"
     CDS             2690..3583
                     /locus_tag="PLC06_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91400.1"
                     /translation="MNPNTNTRLVLFLMLLLVAAALVACGGDEAPEATEATEAVAEVA
                     PVEEAPTEPATAPPTAEPTAAPPTAAPTEAPTEPAVPLFATGYCGHAFYPVVDGRALT
                     YATNSTLEGTGEYTTTFSNVTDTSFTLSTDVGEGTMIATDWTCTADGLLAPQFSQLPG
                     GMEGMDIEFTEADGISIPSEEMFQVGESWPLHYIANATMAVGDDASMQMQQTFDMTNT
                     VTAIEAISVPAGDFPNAVRVETTGSISTAVTMGGQQQPGVSFDLNYTSWYAEGVGLVR
                     QEMPDFFGTGSNYITELVSVD"
     gene            complement(3580..4176)
                     /locus_tag="PLC06_12955"
     CDS             complement(3580..4176)
                     /locus_tag="PLC06_12955"
                     /inference="COORDINATES: protein motif:HMM:NF022747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HRO91401.1"
                     /translation="MGSARSRKKPANKKAVKQETSSNRTVLIALGAAAGVIVLGALLW
                     LALRPEPEIQGVINYPRPSQGHQVNLSIPFGEIPPAGGVHDPVWQNCGIYDTPLNNAH
                     AIHALEHGTAWITYRTDTPAETIAAIQDKFRGQTFTLISPYPEQRSPIVLTTWGTQLE
                     VDDIKDSRVDRFIERYRLGPNTPELGAACTNGIGEPIG"
     gene            4385..>4613
                     /locus_tag="PLC06_12960"
     CDS             4385..>4613
                     /locus_tag="PLC06_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91402.1"
                     /translation="MFARYIITILPTGETLPHPVADESGTLRLELPGRSVTIETPDAV
                     PDTALVIRHEPDQITITEGYPIPATTGGLPHS"
BASE COUNT          707 a         1576 c         1542 g          788 t
ORIGIN      
        1 tggccgccgc ccaatcggcc gtggacgccg cccgcgccgc gctggcccgg atgaccttgg
       61 tggctccatt cgccggaacg gtagcccaaa ttgacgtcga agtgggcgaa ttggtcgccc
      121 cgggtgtggt cgtggcccgc ctcggttcgg ccgcgggttg ggccatggag acgaccgatc
      181 tggtggagct ggacgtgccc gccgtgactg aagggcaggc ggcggtggtg acgctcgacg
      241 cgctgcccaa cgagacgctg cgcgggacgg tgatcgacat cggccgcgtg cccgaaatcg
      301 tgcgcggcga cgtggtctat cgcgtccgca tcgccctcga cgactacccc gatctgccgc
      361 tgcgctgggg gatgacggcg gacgtggtgg gggagcgatg atgggagatt gctttcccgg
      421 aagacagccg gtttccgccc ggaccggata taatcgccgc cgtcggccgt ttttgccgac
      481 cagacctgat ccataatcac tacgttggag cgtaaggaga cagacctccg ccccatgttc
      541 gacatcttca aaaaccttga tcctgtcctc caggccttgc tggcctccgg cttcacctgg
      601 ggcgtgacgg cccttggcgc gtccattgtg tttttcacgc ggacggtcaa ccgcaagctg
      661 ctcgacggca tgatgggctt cgccgccggg gtgatgctcg ccgccagctt ctggtcgttg
      721 ttggccccgg ccatcgaact ggccgagagt aacggcggcc cggcgtggtt gccggcggtt
      781 gtcggcttct cggtcggggg tctcttcctg gccgccgcgg accgcctgat cccccatctc
      841 cacattggcg cgccgctggc cgaggccgag ggtatcaaaa cccaatggca acgcagcgcg
      901 ctgctcattc tggccatcac tctccacaac ttccccgaag ggctggccat cggcgtggct
      961 tttggcgcgg tggcggccgg tttgcctgcg gccacgctgc cggcggcgat cgctctggcc
     1021 atcggcatcg gcatccagaa cttccccgaa gggatcgcgg tagccatgcc gctgcgcgcc
     1081 gaggggatgt cgcggtggaa gagcttcttc tatgggcagg cgtcggcgct ggtggagccg
     1141 gtggcggccg tgctgggggc gagcatcgtc cttgtggccc gcaacgcctt gccctatgcg
     1201 ctgagcttcg ccgccggagc gatggtcttc gtcgtcatcg aggagctgat ccccgaatcc
     1261 caatccggct cgaacaccga tctggctacc acctgcgcga tgggcggctt cgtggtgatg
     1321 atgctgctcg atgtcgccct gggatagcca cccccacatg aaccaaccga acgccatgag
     1381 accttactac accgacagta ccacccttga ttttgcggcg atgatcgagt cgctggaaga
     1441 cttcgacggc cgacccgccg tcctgctcga acccggctac ttctacccca cgtccggcgg
     1501 ccagccccat gacaccggcc aactgcgccg cggcgacgag attgcccgcg tcatcgacgt
     1561 cgtcgagcgc gagagcgacg gcgtggcgct ccacatcctc gatcggccgc tgtcgcccgg
     1621 cccggtggcg gcgagcatcg accgcgaccg ccgcctcgac cacatgcgcc accacaccgg
     1681 ccagcacatc ctgtcgcagg cgttcattcg cgtcgccgag gccgagacgg tcggcttcca
     1741 tatgagcgcg cagtcgatca ccatcgacct cgaccgggcc gacctgagcg cggccgagat
     1801 cgaccgggcg gaggcgctgg ccaacgaggt cgtctgggcc gacgggccgg tgagcgtccg
     1861 cttcgtgacc ctggccgagg cggcaacctt gccgctgcgc aagacgccgc cgggccgcga
     1921 tggtcgcctg cgcctgatcg acatcgaggg gttcgacctg accgcctgtg gcggcaccca
     1981 cgtggcccgg acgggcgagg tggggttgat ccggattgcc cgaacggagc ggcgcggcgg
     2041 gatgacgcgc gtcgagttcc tctgcgcggg gcgggcgctg gccgactatc gcgccggcca
     2101 ggacgtgctg cggcagttgg gcgcggcgct gaccaccggc gcggccgagc tgccggcggc
     2161 cgtggcccgc ttgcaagagg aaaacaaggc gctgcggctc gatctgcggc ggcgcgagga
     2221 tgcgctgctg gcgctggagg cggcgcagct gctggccgcg gccgagccgg cgagcgcggc
     2281 ccgactggtg cggcgcgtct tcgtcggccg ctcgcccgat gaggtgcggc ggctggccac
     2341 ggcgctggtc gaggccggcg gcgtggtggc gctgctgggg ctggccgggg agcgctcgca
     2401 tctggtcttc gcccgttcgg ccgacgcgcc gggcgacatg ggcgcgctgg tcaaggtcgc
     2461 gctggcgaag ctgggcggcc gcggcggcgg cagcccgacg ctggctcagg gcggcggccc
     2521 ggccgccgat gaagcggccg tggcggccgt gctgcaaccg gagctatagc gccctgtagc
     2581 gcaatccgcc ggattgcttc ttttcccccc gcaatctgac agattgcgct acaccattcc
     2641 tgattcccgg tctggacttt acaggcccca ccccgtacaa tcccccgcta tgaacccgaa
     2701 caccaacact cgtctcgtcc tgttcctgat gctgctcctc gtcgcggccg ctctggtggc
     2761 ttgtgggggt gacgaagcgc ccgaggcgac cgaggccacc gaagccgtcg ccgaagtggc
     2821 ccccgtcgaa gaagccccga ccgagccggc aaccgcgccg ccaacggccg aaccaacggc
     2881 cgcgccgccg acggccgcgc cgaccgaggc ccccaccgag ccggccgtcc ccctgttcgc
     2941 caccggctat tgcggccacg ccttctaccc ggtcgtcgac ggccgcgcgc tcacctacgc
     3001 gaccaactcc accctggagg gcaccggcga atacacgacc accttcagca acgtgaccga
     3061 cacctcattc accctcagca ccgacgtggg cgaaggcacg atgatcgcca ccgattggac
     3121 atgcaccgcc gacgggctgc tcgcgccgca gttcagccag ctccccggcg gcatggaagg
     3181 catggatatc gagttcactg aggccgacgg gatcagcatt ccgtcagagg agatgttcca
     3241 ggtcggcgaa tcgtggccgc tccactacat tgccaacgcg accatggccg tgggcgatga
     3301 cgcgtccatg cagatgcagc agactttcga catgaccaac accgtcaccg ccatcgaggc
     3361 gatcagcgtg ccggcgggcg atttccccaa cgcggtgcgc gtcgagacga ccggctccat
     3421 cagcacagcc gtcaccatgg gcggccaaca gcagccgggc gtctcgttcg atctgaacta
     3481 caccagctgg tacgccgaag gcgtcgggct ggtgcgccag gagatgcccg atttcttcgg
     3541 cacgggcagc aattacatca ccgaactggt ctcggtcgat tagccgatcg gctcgccgat
     3601 gccgttggtg caggccgcgc ccagctcggg ggtgttcggg ccgaggcgat agcgctcgat
     3661 gaaccggtcg acgcgactgt ccttgatgtc gtccacctcc agctgcgtcc cccaggtcgt
     3721 cagcacgatg gggctgcgct gctccgggta ggggctgatc agcgtgaagg tctggccgcg
     3781 gaacttgtcc tggatggcgg cgattgtctc ggccggggtg tcggtgcgat aggtgatcca
     3841 cgccgtgccg tgctccagcg cgtggatggc gtgggcgtta ttgagcggcg tgtcatagat
     3901 gccgcagttc tgccagacgg ggtcatggac gccgccggcg gggggtatct cgccgaacgg
     3961 gatgctaagg ttgacctggt ggccctgcga cggccgcggg tagttgatca cgccctgaat
     4021 ctccggctcc ggccgcagcg ccagccacag cagggcgccc agcacgatga cgccggcggc
     4081 cgcgcccagg gcgattagaa ctgtgcggtt ggatgaggtt tcctgcttga ctgctttctt
     4141 gttcgccggc ttcttccggc ttcgtgctga acccatgatg ttgtctcctc aaataagtcc
     4201 ggggtgaccg gcacaaaatc gaacggtata ttgacaaatt tccgcgccgc gcgcaaataa
     4261 acggacgagt ctctcactta acccggagat cgcttttcac gatttatcga tggtagacgc
     4321 ggcccgattt tgttaacatg ggcctcagtc cgtcatacat cctttggttc agggggatta
     4381 tcccgtgttc gcgcgctata tcatcaccat cctgcccacc ggggagacat tgccccaccc
     4441 cgtcgccgac gaaagcggca cgcttcggct ggagctgccc ggccgttccg tcaccatcga
     4501 gacgcccgac gctgtaccgg ataccgccct ggttatccgg catgagccgg atcagatcac
     4561 gatcaccgag ggctacccca tcccggcgac gaccggcggc ctgccccatt cgg
//
LOCUS       DAOMQG010000279         4590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7869_length_4590_cov_9.345976, whole genome shotgun sequence.
ACCESSION   DAOMQG010000279 DAOMQG010000000
VERSION     DAOMQG010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7869_length_4590_cov_9.345976"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="PLC06_12965"
     CDS             <1..1538
                     /locus_tag="PLC06_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HRO91403.1"
                     /translation="LLGRKPPSIVDVDAITVEVITKTLRAWQQGQKPPPDLLALALLS
                     DSVTGTPGGRDIRLRDLLLGMTTAELARARQLEEVPEPEPAATRQGLLNAVARDFANS
                     KGELQAWSALYHRYLAPVELSAEELAAMARVDPRHFRRLVDAGARRLTDHLRRAELAA
                     HVKAHANQLGRHLPPPDYAQLFGVDAPRAELTGLLRRPDGPQLISIEGLGGIGKTALA
                     RAVAFDLAGGNEFDGIAWISARQSRLTELGSIEAMTDAATTLADIIGRLAEQLGLATL
                     VGLDTNEKLAHIAAFLANARYLIVVDNLESVRDIDALLPALITLAAHARILLTSRQAV
                     TGYPLVHRRAVEPLSLEDSRALIEAELNRHRLTLKLDPDDMRSLFAVVGGMPLALRLI
                     AAQLGRRPLHLLLADMQQVHRRAPESLYTYIYRQAWLDLSDPARAMLLSLLHIAPEGE
                     DLEWLQLITALPPDEFDDALDRLLAYSLLDVAGTAAAPRYRMHRLTITFLQTEILSRW
                     EGE"
     gene            1535..3118
                     /locus_tag="PLC06_12970"
     CDS             1535..3118
                     /locus_tag="PLC06_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO91404.1"
                     /translation="MIPSPTRHKWPHERERWAGFYAERLPLQLRQVTAFASARRDKPD
                     TLRLHFESCLTLLEATANRHDLSALWLALVDALHPLPLRWGFWSPWLDVLQRAADKAA
                     ELGRPAMRARYLAHMATLLLDTAQAGAALRRASEALGEAGRAGDGYALALAGSRMAGA
                     LSALGRYDEARELLVEVRYQLDALPTGASGLPEVPGYALLDMEQMDLARQDGRLAEAV
                     VIGERLIAALEATAGIDPHDLATALRRRATILWAAGRYDEAARDLTRSAEMFRAAGDP
                     LAAIFSEGNLGVVYFSMGQFRLAETVKARAIRSAEELNANWWLVRDIGELCGIYMYSG
                     QLERALAYCRRHVELARRLGDERQLALARDNRGVTLMLLGRHDEARPDIEDSLTYFRD
                     EGIIESTVLATLDMALFLRATGEPERAAALAEESYARANRLDSPILRILTTRCLALFR
                     PPAEQERLLREALALAEAHERRMDVAGCLFSLAALENDTAERVRLYNQAAAMLREMEA
                     EAWLAGRSPADPPLLPMFY"
     gene            3185..4555
                     /locus_tag="PLC06_12975"
     CDS             3185..4555
                     /locus_tag="PLC06_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRO91405.1"
                     /translation="MQFKTIIEPFRIKTVEPIRQTTREERAEFLKEAGYNLFLLRAED
                     VIIDLLTDSGTGAMSSGQWAGMMLGDESYAGAKSFYKFEAAVQRITGFKHIIPTHQGR
                     AAERILFGSLLEPGDVVASNTHFDTTRANIEYRQATALDIPNAAAHDPALILPFKGNI
                     DLEALEQTLIEHAGHAPLAMITVTNNSGGGQPVSMANIRAASAICRRHGVLFFLDACR
                     FAENAWFIKMREEGYAAKSPLEIAQEMFSYADGCTMSAKKDGMANIGGFLALNDDALA
                     EKCKTLLILTEGFPTYGGLAGYDLEAIAVGLEEVLHEDYLQYRTRSVAYLGDILTANG
                     VPIVQPPGGHAIYIDALSMLPHLPQSQYPAWALSLALYVEGGIRSVEIGSVMFGHQPD
                     GSEKPAAMELVRMAFPRRVYTQSHVDYLAEVILYVNSIKDTIRGVKIVEAPEVLRHFS
                     ARMGMV"
BASE COUNT          792 a         1538 c         1496 g          764 t
ORIGIN      
        1 gacttcttgg gaggaagcca ccctctatcg tggatgttga tgccatcact gtcgaagtga
       61 tcaccaaaac cctgcgcgcc tggcagcagg ggcagaaacc accaccggat ttgctggccc
      121 tggccctgct gtcggactcc gtaaccggca cgcccggcgg ccgcgatatt cgcttgcgcg
      181 atctcctcct gggtatgaca accgcggaac tggcccgggc gcgacaattg gaagaagtgc
      241 cggagccgga gccggctgcc acgcgccagg gcctcctcaa cgccgtcgcc cgcgatttcg
      301 cgaacagcaa aggcgagttg caggcctgga gcgctcttta tcatcgctat ctggcccctg
      361 ttgaactcag cgcggaggag ctggccgcga tggcccgcgt ggacccgcgc cactttcgcc
      421 gcctggtcga cgcgggcgcg cgccgtctaa ccgatcactt gcggcgggcc gagctggcgg
      481 cccatgtgaa ggcccatgcc aaccaattgg gccgccactt gccgccgccc gattatgcgc
      541 agttgttcgg cgtcgatgcg ccgcgggcgg aattgaccgg cctgctgcgc cggccggacg
      601 gcccgcaact catcagcatc gaagggctgg gcggcatcgg caagacggct ttggctcgcg
      661 cggttgcctt cgatctggcc ggcggcaatg agttcgacgg catcgcctgg atcagcgccc
      721 gacaatcccg gctgaccgag ttggggagca tcgaggccat gaccgacgcc gccacaacgc
      781 tggccgacat catcggccgc ctggcggagc aactcggtct ggcgacgctg gtggggctgg
      841 acacgaatga aaaactggct catatcgccg cgttcctggc caacgctcgc tacctgatcg
      901 tcgtcgacaa tctggagagc gtccgcgaca tcgacgcgct gctgccggcc ctgatcacgc
      961 tcgccgcgca tgcccgcatc ctgctcacca gccgccaggc ggtgaccggc tatccattgg
     1021 tccatcgtcg cgcggttgaa ccgctctctc tggaggatag ccgggcgttg atcgaggccg
     1081 agttaaatcg ccaccggctg accctgaaac tcgaccccga cgacatgcga agcctgttcg
     1141 ctgtggttgg cgggatgcct ctggcgctga ggctcatcgc cgcgcaattg ggccgccggc
     1201 cattgcatct cttgctcgcg gatatgcagc aggttcaccg gcgcgccccg gaaagcctct
     1261 atacctacat ataccgccag gcatggcttg atttgagtga tccggcgcgg gccatgctgc
     1321 tgtccttgct ccacattgca cccgaagggg aagacctgga atggctccaa ttgataaccg
     1381 cgctgccgcc cgatgagttc gacgacgcgc tggaccggtt gttggcctat tcattgctcg
     1441 acgtggccgg taccgcggcc gcgccgcgct accggatgca ccgcctgacg atcacatttc
     1501 tgcaaacgga aatcctgtcc cggtgggagg gggaatgatc ccgtccccca cgcgccacaa
     1561 atggccccac gagcgcgagc gctgggcggg cttctacgcc gaacgcctgc cgctccaatt
     1621 gcgccaggtg accgccttcg cctcggcccg acgcgacaag cccgacaccc tgcggctgca
     1681 cttcgagagc tgcctgaccc tgcttgaggc cacggccaac cgccacgacc tgtcggcgct
     1741 gtggctcgct ctcgtcgacg cgctccaccc cttgccgttg cgctggggct tctggtcgcc
     1801 ctggctggac gtgttgcagc gggcagcgga taaagcggcc gagttgggcc gcccggccat
     1861 gcgcgcccgc tatctggccc acatggccac gttgcttctg gacaccgcgc aggccggcgc
     1921 agccttgcgg agggcgagcg aggcgctggg tgaggccggc cgcgccggtg acggctatgc
     1981 gctggcgctg gccggcagcc ggatggccgg cgcattgagc gcgctggggc gctatgacga
     2041 agccagggag ctgctggtcg aagtcagata tcaattggac gccctgccga ccggcgcatc
     2101 cggcctgccc gaggttccgg gctacgcctt gctcgatatg gaacagatgg acctggcccg
     2161 gcaggacggg cggttggccg aggccgtcgt aatcggcgag cgtctgatcg cggccctgga
     2221 agcgacggcg ggcatcgacc cccacgacct ggcgactgcc ctccgccggc gggcgacgat
     2281 cctgtgggcg gccgggcgat atgacgaagc ggccagggat ttgactcgat cggcagagat
     2341 gtttcgcgct gcgggcgacc cgctggcggc gatcttctcc gaggggaacc tgggcgtggt
     2401 ctatttcagc atggggcagt ttcggctggc cgaaacggtg aaggcccggg ccattcgctc
     2461 ggccgaggaa ctcaacgcca actggtggct cgtgcgcgac atcggcgagt tgtgcggcat
     2521 ctacatgtac agcgggcaac tggaacgagc gctggcctat tgccggcgcc acgtcgaact
     2581 ggctcgccgg ctgggtgacg agcggcaact ggctctggcg cgggacaacc gcggcgtcac
     2641 gctcatgctg ctcggccgcc atgacgaagc gcgaccggat atcgaagaca gcctgaccta
     2701 cttccgggac gaagggatca tcgagagcac ggtgctggcg acgctggaca tggctctctt
     2761 cctgcgggcc acgggcgagc cggagcgggc ggccgcgttg gccgaggaga gttatgcgcg
     2821 ggcaaaccgg ctggattccc ccatcctgcg catcctgaca acccgctgcc tggcgctgtt
     2881 ccggccgccg gccgagcagg agcggttgct gcgtgaggcg ctggccctgg ccgaagcgca
     2941 cgaacgccgc atggacgttg ccggctgcct gttctcgctg gcggcgctgg aaaacgacac
     3001 agccgagcgc gtccggctct ataaccaggc ggccgccatg ttacgcgaga tggaagccga
     3061 ggcctggctg gcgggtcgtt cgcccgccga cccgccgctt ttgcccatgt tctattgata
     3121 ttcggatgcg ccggcgggta atcgagctac aatcccctga cccacattaa tccggagaac
     3181 caccatgcaa ttcaaaacca ttatcgaacc ctttcgcatc aagaccgtcg agcctatccg
     3241 ccagaccact cgcgaggagc gggcggagtt tctgaaggaa gcgggctata acctgttcct
     3301 gctgcgggcc gaggacgtga tcatcgacct gctgaccgac tccggcacgg gggcaatgtc
     3361 ctccggccaa tgggcgggta tgatgctggg tgacgagagc tacgctgggg cgaagtcgtt
     3421 ctataagttc gaggccgccg tgcagcgcat caccggcttc aagcacatca tccccaccca
     3481 ccaggggcgc gcggccgagc gcatcctgtt cggctcgctg ctggagccgg gcgatgtagt
     3541 ggccagcaac acccacttcg acaccacccg cgccaacatc gaatatcgcc aggcgacggc
     3601 gctcgatatc cccaacgcgg ccgcccacga tccggcactc atcctgccct tcaaaggcaa
     3661 catcgacctg gaggcgctgg agcagacgct gatcgagcac gccgggcacg cgccgctggc
     3721 gatgatcacc gtcaccaaca actccggcgg cggccagccg gtcagcatgg ccaacattcg
     3781 cgccgcgagc gcgatctgtc gtcgccacgg cgtgctgttc ttcctcgacg cctgccgctt
     3841 cgccgagaac gcctggttca tcaagatgcg cgaagaaggc tacgccgcca aatcgccgct
     3901 ggagatcgcc caggagatgt tcagctacgc cgacggctgc acgatgagcg ccaagaaaga
     3961 cggcatggcc aacatcggcg gtttcctggc cctcaacgac gacgcgctgg ccgagaagtg
     4021 caaaaccctg ctcatcctga ccgagggctt ccccacctat ggcgggctgg cgggctacga
     4081 cctggaagcc atcgccgtgg ggctggagga ggtgctgcac gaggactacc tgcaataccg
     4141 cacgcgctcg gtggcctatc tgggcgacat cctgacggcc aacggcgtgc ccatcgtcca
     4201 gccgcccggc ggccacgcca tctacatcga cgcgctgagc atgctccccc acctgcccca
     4261 gagccagtat ccggcctggg cgctgtcgct ggccctctac gtggaaggcg gcatccgctc
     4321 ggtggagatc ggctcggtga tgttcggcca ccagcccgac ggcagcgaga agcccgcagc
     4381 catggaactg gtacgcatgg ccttcccgcg ccgagtgtac acgcaaagcc acgtcgatta
     4441 tctggccgag gtgatcctct acgtcaacag catcaaggac accatccgcg gcgtgaagat
     4501 cgtggaagca ccggaggtgt taaggcattt ttcggcgcgg atggggatgg tttagtggta
     4561 tagaaaccgg gtttcccgga agaaacccgg
//
LOCUS       DAOMQG010000280         4587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_7875_length_4587_cov_12.563769, whole genome shotgun sequence.
ACCESSION   DAOMQG010000280 DAOMQG010000000
VERSION     DAOMQG010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7875_length_4587_cov_12.563769"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1540)
                     /locus_tag="PLC06_12980"
     CDS             complement(20..1540)
                     /locus_tag="PLC06_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRO91406.1"
                     /translation="MSNLKLRAARGSLYMTVVSFGMRPFSMILAILLARLLSPADFGL
                     LAMAMLIFNAANLFTDMGMRPTVVQTKEDINKVAHYAFVITMGASIAFTLIAILIAEP
                     LSAFLGGSAQLPSIIRWMAIYVTIDGLWIIPEALLRRDLRFKELSLSQIPGEIASVII
                     AIPLALMGFGVWSLVIGNCLGQLLRAALLWSYYRPWIWLRPQKWDWPIVRGMLKFGLP
                     SLGSGLLRYVQNQIDDFILGRRLGPTPLGYYNKAWNLTTRLSDMLTTSIFGNVLFPSY
                     AKIQDDKERLSRAYLKSTKMVLLMIVPVSVGLAITARLLVPVLLGPKWAPMIPIWELF
                     SLYGLTRPISTNSAPIFLAVGQPGRNLTASAVLLGVMLPLIFWLIDPYGAIGVAIAVS
                     VASAVVMLFNIYQVNQILPKTAVRTIVQSVPFLIAGGLMGISVVLLQPSIITLAGGEN
                     LISLALVMLVGAITYLGAILLLQRDLILELYELAIRALNLDRRWPRLLPARLRANK"
     gene            complement(1607..2764)
                     /locus_tag="PLC06_12985"
     CDS             complement(1607..2764)
                     /locus_tag="PLC06_12985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91407.1"
                     /translation="MKILYIWFYSRSFPDYPKVAAVLRERGHEAWVANYDDAGDIVWY
                     RGDELVAKVEGPGRAPRSVGRIPVLRSLWHWFAFVRFMLRLRGFFRRQNADIVHVCPN
                     AARMVWLLPLGMPRHMRFVIDYRQIAQREGRGLIGRLKSGFANGLRVLYCRVFYDRAT
                     FLHALGAEKVLGPEWARWADVVPLAVDEPFLSMPNEPRDSGRGPVTFLYLGSIARIRK
                     LEQIMAAAELVTADTHNFRLDFIGPDVAGGYYHGLVKAMGLERVVRILPPIPYDSVPE
                     TVTTYDVALTIVPENPSDWQYQPTIKGIEYRALGMPAIATDFAPNRELVINEVNGLLV
                     ANTAASIAEAMRRFICEPGFLAQAQGNARAMREGPTWDEIATRYMDIYERL"
     gene            complement(2780..3796)
                     /locus_tag="PLC06_12990"
     CDS             complement(2780..3796)
                     /locus_tag="PLC06_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRO91408.1"
                     /translation="MKVVVLMGGYGTRMRPHSWSRPKPVMQVANNTVIGHILDRFAEV
                     ATEEVVLVVGYKGEQIESWARAHYAHLDLRFVYQHEPLGQAHALWLCRDFLDGGEVVV
                     MLGDIVIDADFGRIAIDAPADADAVFQTMEIDNPRQFGCLTVDADGFIDRIVEKPETG
                     DYHLALAGVYWFRDSARLQQTLDQTLQSGRRTKGEYYLADTFQLMLERGDHIGVQPIR
                     SIVDVGSPTNRLDANRRLLQDNATHQKPPTGLSDSVVVVPPVYIHPTAVVERSVVGPY
                     ATICQDARVRDSVIADSIIDPDAIVSDIILRDSIIGENAVVEGESRALIVGDDSRMTM
                     AGRR"
     gene            complement(3817..>4587)
                     /gene="galE"
                     /locus_tag="PLC06_12995"
     CDS             complement(3817..>4587)
                     /gene="galE"
                     /locus_tag="PLC06_12995"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRO91409.1"
                     /translation="IMHFASYTLVGESWEKPFLYLRDNIVNGLNLLESAAEHGVRRFI
                     LSSTANLFDDPENMPIHETERIVPGSPYGESKNIMERYLHWMDRVYGMRYACLRYFNA
                     CGATEERGEHHDPETHLIPLVLQVALGQRDKITIFGDDYNTPDGTCVRDYIHVVDLAE
                     AHILALGAVDGGSRKYNLGNGRGFSVKEVIETAREVTGHPIPAVIGPRRPGDPDILIA
                     GSDAIVRDLGWQRQYPDLRRIIEMAWKWHQAHPNGYSH"
BASE COUNT         1099 a         1358 c         1286 g          844 t
ORIGIN      
        1 gatcgttaac gtaaagagct tatttatttg cgcgcagacg agccggcaaa agcctgggcc
       61 aacgacgatc caggtttaat gctctgatag cgagctcata aagctccagg atcagatcgc
      121 gctgcaataa aaggattgca cccaaatagg ttatagctcc cacaagcata accagggcca
      181 aggagatgag gttctcgcca ccggccaagg taatgattga cggctgaagc aacaccacgc
      241 ttatacccat caacccgccg gcaatcagga aaggcacact ttgaacaatg gtccgaactg
      301 cggttttggg cagtatctga ttcacctgat aaatattgaa caacataacg accgcgctgg
      361 cgacggagac agcgatggcc acaccaatcg caccataggg atcaatgagc caaaagatga
      421 gcggtagcat caccccaagc agcaccgcgc tggccgtcag gttgcggccg ggctgcccca
      481 ccgccagaaa aatgggggct gaattcgtcg atatgggacg agtcagccca tagagcgaga
      541 aaagttccca gattgggatc atcggtgccc attttggccc taataaaaca ggcaccaaca
      601 gccgagcggt aattgctaac ccgacggaaa caggaactat catcaggaga accatcttgg
      661 tactttttag gtaggcgcgt gacaggcgct ctttatcgtc ctgaatcttg gcataggagg
      721 ggaacaatac gttgccaaag atcgatgtcg tcagcatatc actcagccga gtcgtcagat
      781 tccacgcctt gttatagtac cctaacggag tcggcccaag gcgacggccg aggatgaagt
      841 catcgatctg attttggaca taacgtaaca atccgctacc cagactcggc aacccaaact
      901 tcagcatccc tcggacaatt ggccaatccc acttttgagg gcgcagccaa atccacggcc
      961 gatagtaaga ccataacaaa gcggcacgca acagctgccc caggcaattg ccgatgacca
     1021 ggctccagac cccaaatccc atgagcgcga gcggaatagc gatgatcacc gaggctatct
     1081 cgcctgggat ttgagaaagg ctcagctctt tgaaacgtaa atcgcggcgc aacagggcct
     1141 cagggataat ccacaaccca tcgatagtta cgtagatagc catccagcga atgatacttg
     1201 gcagttgcgc actgccaccc agaaatgcag ataaaggttc agctatcagt atggcgatta
     1261 gcgtgaaggc gatgctggct cccatagtga taacgaaagc atagtgggcg accttgttaa
     1321 tatcttcttt cgtctgcacc acagttgggc gcattcccat gtcggtaaac aggttagccg
     1381 cgttgaagat gagcatcgcc atagccagca gaccgaaatc ggcgggactg agcaaacgtg
     1441 ccaacaggat ggccagaatc atggagaacg gccgcatccc gaaggaaaca accgtcatat
     1501 agaggctgcc gcgagccgcc cgcaatttca aattactcaa tgcaacacct cacccaatcg
     1561 atatcgctcc gctccgggac aatgaaaagc ccaggttcca aagcgattat aatcgctcat
     1621 agatatccat gtaacgagtc gcgatctcat cccaggtcgg cccctcgcgc atggcgcgcg
     1681 cgttgccttg tgcctgcgcc agaaaccccg gctcgcagat gaaacgacgc atggcttcag
     1741 cgatactcgc agcagtgtta gccaccagca atccattgac ttcgttaatc acgagttcgc
     1801 ggttgggggc gaagtcggtg gcgatggccg gcatccccag agcgcgatac tcaataccct
     1861 tgatagttgg ctggtattgc cagtccgacg gattttcggg cacgatagtc agagcaacat
     1921 cataggtcgt caccgtctcc ggcacgctgt cataggggat aggcggcaat atgcgcacca
     1981 cccgctcaag ccccatcgcc ttcaccaacc cgtgatagta gccgccggcc acatcgggac
     2041 cgatgaaatc aagccggaaa ttatgcgtgt cggccgtcac caactcagcg gcggccatga
     2101 tctgctccag tttgcggatg cgggcgatag atcccagata gaggaaagta acaggccctc
     2161 gtccgctgtc acgcggttca ttgggcatgg acaggaaagg ctcgtccacg gccaacggca
     2221 ccacgtctgc ccagcgcgcc cactccggcc ccagcacctt ctccgcgcca agagcgtgga
     2281 gaaaggtggc ccgatcatag aagacgcggc aatagagcac gcgcaagccg ttagcaaagc
     2341 cgctcttcag gcggccgatc aatcctcgtc cctcacgctg ggcgatctgc cgatagtcga
     2401 tgacgaaacg catatggcgc ggcatgccca acggcagcag ccacaccatg cgtgccgcat
     2461 tggggcacac atggacgatg tcggcgtttt gacggcggaa gaaaccgcgc agccgcagca
     2521 tgaaacgcac aaaggcgaac cagtgccaca gcgagcgcaa caccgggata cgaccaaccg
     2581 aacgcggtgc gcggcccggt ccctctactt tggccaccag ttcgtcgcca cgataccaga
     2641 cgatatcgcc ggcatcatca tagttggcga cccacgcctc atgaccccgc tcacgcagca
     2701 cggccgccac cttgggatag tcggggaaac tgcgcgagta gaaccagata taaagtatct
     2761 tcatctcgtc accctggaat caccggcggc cagccattgt catccggcta tcgtcgccaa
     2821 cgatgagagc gcgggactca ccctcaacga cggcgttctc gccaatgatc gagtcgcgca
     2881 ggatgatgtc ggagacgatt gcgtcgggat cgatgatgct gtcggcaatg accgaatcgc
     2941 gaactcgcgc atcctgacaa attgtggcat aagggccgac gaccgaacgc tcgacaacgg
     3001 ccgtcgggtg aatatagacc ggcggcacaa cgacgaccga atcgctcagt ccggttggtg
     3061 gcttctgatg cgtggcgtta tcctgcaaca atcggcggtt ggcatccaga cgattggtcg
     3121 gcgagccgac atccacgatg gaacggatcg gctgaacacc aatgtggtcg ccccgttcca
     3181 gcatgagctg gaaggtgtcg gccaggtaat actcgccctt tgtccgccgg ccggactgga
     3241 gcgtttgatc gagcgtctgt tgcaaccggg cgctatcgcg aaaccagtag accccggcca
     3301 gcgccaggtg ataatcaccc gtttcgggtt tctcaacaat gcggtcgatg aaaccgtcgg
     3361 cgtcgaccgt cagacagcca aattgacgag gattgtcgat ctccatcgtc tgaaaaacgg
     3421 catcggcatc ggccggggca tcaatagcaa tgcggccgaa gtcggcatca atgacgatat
     3481 cgcccagcat gacgacgacc tccccgccat cgaggaaatc acggcaaagc cacagcgcgt
     3541 gagcctgacc cagcggctca tgctggtaga cgaagcgcag gtcaaggtgc gcgtaatgcg
     3601 cgcgcgccca cgattcgatc tgttcgccct tgtagccaac gacaaggacg acctcttcgg
     3661 tggcgacctc ggcgaaacga tcaaggatat ggccgatcac ggtgttgttc gccacctgca
     3721 tcaccggctt gggccggctc caggagtgcg gccgcatccg tgtgccgtat ccacccatca
     3781 aaaccacgac tttcattgtc tttctccgcg acaggattag tggctgtaac cgttcggatg
     3841 ggcctgatgc catttccagg ccatctcaat gatccggcgc aggtcggggt attgacgctg
     3901 ccaacccaga tcgcgcacga tggcgtcgct gccggcaatg agaatatcgg gatcgcccgg
     3961 ccggcgcggc ccaatgacgg ccggaatggg atggccggtc acctcgcgcg cggtctcgat
     4021 aacttccttc acgctgaagc cgcggccgtt gcccagatta tatttccggc tacccccatc
     4081 gaccgcccct agcgccagga tatgggcctc ggccaggtca acgacgtgga tgtagtcgcg
     4141 cacgcaggtg ccgtcgggcg tgttgtagtc gtcgccgaag atggtgatct tgtcccgctg
     4201 ccccagggcg acctgaagca caagggggat gaggtgggtc tccgggtcat gatgttcgcc
     4261 gcgctcttcg gtcgcgccgc aggcattgaa gtagcgcagg caagcatagc gcatgccata
     4321 aacgcgatcc atccagtgca gataacgctc catgatattc ttcgactcgc cgtaagggct
     4381 gccgggaacg atgcgctccg tctcatggat gggcatgttt tcggggtcgt cgaacaggtt
     4441 ggccgtcgag gagaggataa agcgacgcac gccatgctcg gcggcgctct ccagcaggtt
     4501 caagccattg acgatgttgt cgcgcaaata caggaaaggc ttctcccacg actcgcctac
     4561 gagcgtgtag gacgcaaagt gcatgat
//
LOCUS       DAOMQG010000281         4511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8035_length_4511_cov_8.129488, whole genome shotgun sequence.
ACCESSION   DAOMQG010000281 DAOMQG010000000
VERSION     DAOMQG010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8035_length_4511_cov_8.129488"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..507
                     /locus_tag="PLC06_13000"
     CDS             286..507
                     /locus_tag="PLC06_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91410.1"
                     /translation="MIDHYPAVHGDILPFILRKELLALARINRLLALHLFPLGMLMNG
                     GKGTNHHVNQHIRYGTTDPLHSYFHLLIE"
     gene            complement(942..>4511)
                     /locus_tag="PLC06_13005"
     CDS             complement(942..>4511)
                     /locus_tag="PLC06_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRO91411.1"
                     /translation="RSAPSEERRSAPSEERRSTPLKPYSKPETPPVGPDTISLLDLMA
                     EEAEEQGQKTHVLPPEIKTVELPPLVPSGSSTRPSQPPAGAAAPAADDDQPTPTVTIL
                     PPGPTDSTPTPTTPGRTEQPLTRPSFDAPATTPTVDDPDATRVQPRVAFPGATQTRPP
                     EAKPGTPPATGEQTTQIHQRPPAGQRPPAGQATPPAGRPMPTSQPRRAATPPPAGERR
                     PAQRPPAARPPAAPPPPVTSRAKRPMTAGRVLRGLLVMLLVLFVGALLAASAAAIGYS
                     LVAIDLPRPTELRNRASSFETARVYDRNGQLLYALADPNEGNRTYVPLDRISPWLVQA
                     TIDTEDKRFYENPGFDLVALVRAVGMAAREQEAVAGTSTITQQLVRATLLSEEERVER
                     SYRRKVREIILAAEISRTYSKEEILELYLNEIYYGNLAYGIEAAANTYFGKSAADLTL
                     AEASLLAGLPQAPALWDPYVAPDKALGRQREVLSLMVANDHITLADAQAALDEMAVRI
                     YEMRPPQVTILHPHFTFTVLQQAEELLGAQSIYRGGLSIRTTLDPDAQRLAEEAVAAN
                     RDALAAGGANNAALVALQPQTGEIMAMVGSADFNNEAISGQVNMALAPRQAGSSIKPL
                     VYLSAFERGWTPATLLWDLQTEFPDGANPPYIPKNYDDQFHGPVRVRPALGNSYNIPA
                     VKALEFVGVCDFINNMQKVGLTDLQDSGCAETGQPREHGLALSLGAGEVSPLQMAGAF
                     GALANGGRFIAPYAISRIEDRNGNVLYDNSSPASVAPQVVRPEHAYLLTGILSDNNAR
                     LEEFGQNNNLVIPGYQIAAKTGTSGTTANDVRDGWTIGYAPQVLVSVWVGNTDNRPIG
                     AGQSGYLLASPIWNRFMTSYLGGREALAFVRPPGIIDREICADTGIAVDATSTCAARM
                     TEIFAGDQPPQDPSTGITRMPIDLWTGLQANPSCSDAVYEATFGGGVPVNGRPDVLER
                     ERALATAWMQTTPAGQQWAAAQGSTSAAISPPTQACDGNTPRPRAEIIRPRSENDALD
                     RAAIEIWGTALGPNYQGYQVEYGLGENPGGWGMVQERRPEALENGLLAVWNASQVDYS
                     GPITIRVIVFGPDNPFTPDYDPVLKEGRTVFNLRQPTPTPTTTPTATPTATPTGTATP
                     TITPTPTATTTGTATPTPTPTLPVTPEAPTVEPSPTPELSPTP"
BASE COUNT          647 a         1383 c         1642 g          839 t
ORIGIN      
        1 tgctgaacga cttctacctc tatgaggatg gcgcgttccg gcggctggac gcggccgaag
       61 cgccgccgct ggggctggag ttggggcggc ggccgttcgc cgtgctgccc gcgccgattg
      121 ggtttccggg ggaagcggcg tgtggggatt aggctagtcg acaaatcgaa gagagcgaga
      181 ccgtagcacc ctgtcaggct cacgtgtaac caacctgata tgcacaatct cgtccaaaga
      241 atatcggaca tcgattcgtt gcgtgagggt tgatatgtcc gaatcgtgat cgaccactac
      301 cccgccgtgc atggcgatat attgcccttc atacttcgaa aggagctcct cgcgctggcg
      361 cgcataaacc gcttgctcgc gctccatctg ttcccgctgg gcatgctcat gaacggaggg
      421 aaaggcacca accaccacgt caaccagcac atccgctatg ggacgactga cccgctgcac
      481 agctatttcc acttgcttat agagtgagtc cggtagcgtc aatgtaacct gtgtacccat
      541 accctctatt ttatcacact cccctgcctc atcattattc aatctcccct acggcagggt
      601 ttttcaatag tccgttccaa ttgtgcaaag cggccttata tggcatgaca caggcatgta
      661 acatattgaa tatttacaaa ttaatcacgt atacgccccg gcggattgaa atccgctgct
      721 gatagacaaa accggttgaa accggttgcc ggcagaggcg gcaaccgact tcagtcggtt
      781 tggcataaca gccgcgggat tcatcccgcg tttgattgat acgtgattat tttgttaaca
      841 tgcaacatgt aaccccggtt tcttaaccgg gtgaataccc ttgctgcggc gccggataag
      901 aatccggcgt tacatcggcc gactactgaa aaaccgtgcc cctacggcgt cggcgataat
      961 tccggtgtcg gcgatggctc cactgtcggc gcttccggcg tcaccggcag ggttggcgtg
     1021 ggtgtcgggg tggccgttcc ggtggtcgtc gccgtggggg tgggggtgat ggtcggggtg
     1081 gccgtgccgg tgggcgtggc ggtcggggtt gcggtcggcg tcgtcgtggg ggtgggcgtc
     1141 ggctgccgca gattgaacac cgtccggcct tccttcagca cggggtcata atcgggcgtg
     1201 aacgggttgt cggggccgaa gacgatgacc cgaatcgtta tcgggccgct gtaatccacc
     1261 tgcgaggcat tccacacggc cagcagacca ttctccagcg cttccggccg ccgctcctgc
     1321 accattcccc agccgccggg gttctcgccg aggccgtatt ccacctgata gccctgatag
     1381 ttcgggccta atgccgtgcc ccatatctcg atggcggcgc ggtcgagggc gtcgttctcg
     1441 ctgcgcggcc gaatgatctc ggcccgcggc cggggcgtgt tgccgtcgca ggcctgcgtc
     1501 ggcggggaga tggccgccga tgtcgaaccc tgcgccgcgg cccactgctg cccggcgggg
     1561 gtcgtctgca tccaggccgt cgccagggcg cgctcgcgct ccagcacgtc ggggcggccg
     1621 ttgaccggca cgccgccgcc gaatgtcgcc tcatacacgg cgtcgctgca cgacgggttg
     1681 gcctgcaggc cggtccacag gtcgatgggc atgcgcgtga tgccggtgga cgggtcttgc
     1741 ggcggctggt cgccggcgaa tatctcggtc atgcgcgcgg cgcaggtcga ggtggcatcg
     1801 acggcgatgc ccgtgtcggc gcagatctcg cggtcgatga tgcccggcgg ccgaacaaag
     1861 gcgagcgcct cgcggccgcc gagatacgac gtcatgaacc ggttccagat gggcgaagcc
     1921 agcaggtagc ccgactgccc cgcgccgatg ggccggttgt cggtgttgcc cacccagacc
     1981 gagaccagca cttgcggcgc gtagccgatg gtccagccgt cgcgcacgtc gttggccgtg
     2041 gtgccgctgg tgcccgtctt ggccgctatc tgatagccgg ggatgaccag gttattgttt
     2101 tggccgaact cttccaggcg ggcgttgttg tcgctgagga tgccggtcag caggtaggcg
     2161 tgttccgggc gcacgacctg cggcgcaacg gaggccggcg acgagttgtc gtagagcacg
     2221 ttgccgttgc ggtcctcgat gcggctgatg gcgtaggggg cgatgaagcg gccgccgttg
     2281 gccagcgcgc cgaatgcccc ggccatctgc agcggcgaga cttcgcccgc gccgagggac
     2341 agcgccaggc catgctcgcg cggctgcccc gtttccgcgc agccggagtc ctgcaaatcg
     2401 gtcaggccaa ccttttgcat gttgttgatg aaatcgcaga cgcccacgaa ctccagcgcc
     2461 ttgaccgccg ggatgttgta ggagttgccc agcgccgggc gcacgcgcac cgggccgtgg
     2521 aactggtcgt cgtagttctt ggggatatag ggcggattcg cgccgtcggg gaactccgtt
     2581 tgcaggtccc acagcagcgt ggccggcgtc cagccgcgct cgaaggcgga caggtagacc
     2641 aggggcttga tcgacgaacc ggcctgtcgc ggggccaggg ccatgttgac ctggccgctg
     2701 atggcctcgt tgttgaagtc ggcgctgccg accatggcca tgatctcgcc cgtctgcggc
     2761 tggagggcca ccagtgcggc gttgttggct ccgccggcgg ccagcgcgtc gcggttggcg
     2821 gcgacggctt cctcggccag gcgctgggcg tcggggtcga gcgtggtgcg gatgctgagg
     2881 ccgccgcgat agatcgactg cgcgccgagc agctcctcgg cttgttgcag cacggtgaag
     2941 gtgaagtggg ggtggagaat ggtcacctgc ggcggccgca tctcatagat gcgcacggcc
     3001 atctcgtcca gcgccgcctg cgcgtcggcc agggtgatgt ggtcattggc gaccatcagg
     3061 ctgagcacct cgcgctggcg gccgagcgcc ttgtcggggg cgacataggg gtcccacagc
     3121 gcgggggcct ggggcagacc ggccagcagc gacgcctcgg ccagcgtcag gtcggcggcc
     3181 gatttgccga aataggtgtt ggccgccgcc tcgatgccgt aggccagatt gccgtagtag
     3241 atctcgttca ggtacagctc aaggatttcc tctttgctat aggtacggga gatctcggcg
     3301 gcgaggatga tctcgcgcac cttgcggcgg tagctgcgct cgacgcgctc ctcctcgctc
     3361 agcagcgtgg cgcgcacgag ctgctgggtg atggtgctcg tcccggcgac ggcttcctgc
     3421 tcgcgggcgg ccatgcccac ggcgcgcacg agggcgacga ggtcgaagcc ggggttctcg
     3481 tagaagcgct tgtcttcggt gtcgatggtg gcctgcacca gccaggggga gatgcgatcc
     3541 agcggcacgt aggtgcggtt gccctcgttg gggtcggcca gggcgtagag caattggccg
     3601 ttgcggtcgt agacgcgggc ggtctcgaaa ctgctggccc ggttgcgcag ctccgtcggc
     3661 cgcggcaggt cgatggccac caaactatag ccgatggccg cggccgaggc ggccagcagc
     3721 gcgccgacga acagcaccag cagcatgacc agcagcccgc gcaggacgcg cccggcggtc
     3781 atcgggcgtt tggcccggct cgtcacgggt gggggcggcg cggcgggcgg gcgggctgcg
     3841 ggcggccgtt gcgcggggcg acgttcgccg gcgggcggag gagtcgcggc ccggcgtggc
     3901 tgggatgtcg gcatcggtcg cccggccggg ggcgtagcct gcccggccgg tggccgttgc
     3961 ccggcgggcg gccgttgatg aatttgcgtc gtctgctcgc ctgtggcggg gggcgttccc
     4021 ggcttggcct ccggcggccg cgtctgtgtg gcgccgggga aggcgacgcg cggctgcacc
     4081 cgggtggcgt cggggtcgtc gacggtgggc gtcgtcgcgg gcgcgtcgaa cgatggccgg
     4141 gtcaggggct gttcggtgcg acccggggtg gtgggggttg gtgtggaatc ggtcgggccg
     4201 gggggcagta tggtgacggt cggcgtgggc tgatcgtcat cggcggccgg ggcggccgct
     4261 ccggccggtg gctgcgaggg ccgcgtcgat gagccggacg ggaccagcgg cggcaattcc
     4321 accgtcttga tctccggcgg caggacgtgg gttttctggc cctgctcttc cgcctcctcg
     4381 gccatcaggt caagcaggga gatggtgtcc ggccccacgg gcggtgtttc gggcttgctg
     4441 tatggcttca ggggcgtgga gcgccgctct tcgctcggcg ctgagcgccg ctcttcgctc
     4501 ggcgctgagc g
//
LOCUS       DAOMQG010000282         4502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8057_length_4502_cov_8.750843, whole genome shotgun sequence.
ACCESSION   DAOMQG010000282 DAOMQG010000000
VERSION     DAOMQG010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_4502_cov_8.750843"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLC06_13010"
     CDS             <1..349
                     /locus_tag="PLC06_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91412.1"
                     /translation="CHLRDVEREVHQSRIRALLLEDGAFLPGIDADQWAIPRNYRGQV
                     GRAALSGFLAARDETIALLSPLSPEVWARQGQHSFFGPTSLQELVFLAVQHDRIHARQ
                     IEALRQASDDKTE"
     gene            complement(368..1348)
                     /locus_tag="PLC06_13015"
     CDS             complement(368..1348)
                     /locus_tag="PLC06_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HRO91413.1"
                     /translation="MSAENYVLVSVLAAALPVLIYIGLIYWVDHYEKEPVWLLSAAFV
                     WGAIPAALLALFFNTLFSLPFYALLSESTAELISGGAIAPVVEEFVKGLALFLILIIQ
                     RNELDSPLDGIIYGAMVGMGFAMVENVLYYTTYFNESGALGWNELVFVRGVIFGLNHA
                     LYSSLTGLGIALARMSRNWAVRIAAPLLGLMAAMALHAFHNVAMFTGSIAGFLAGLTF
                     GWGGVMLTVAIIILSLYQERRWMRHYLAEEVGLGTLSAEELAIVSSAAQRGRRRMRLL
                     FDEGVEAYRLAGQRYQMLSELAYRKRHHAHLQDAAGAQAIITLRQSITEL"
     gene            complement(1414..2067)
                     /locus_tag="PLC06_13020"
     CDS             complement(1414..2067)
                     /locus_tag="PLC06_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRO91414.1"
                     /translation="MVGWRQLVGLAVAAVLLVGAGVGLARLRGAAQPAPIEIMPPEAT
                     QASSPATAPMTETSPTDGPIHIYVSGAVLRPAVYTLPPGSLLDDAVRAAGGFAAGADT
                     AAVNLAQPAADGMQVHVPLLAEALPTPPPVSLPSGAEPTVAGRMGGISISGLVNINTA
                     TQTDLEMLPGIGPGTAINIIAHREANGPFATIEDIMDVPGIGDGKFEAIRDLITVGP"
     gene            complement(2109..3092)
                     /locus_tag="PLC06_13025"
     CDS             complement(2109..3092)
                     /locus_tag="PLC06_13025"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013351728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRO91415.1"
                     /translation="MLFVDNKGITDPRLNLAFEEYLLRHVRETEPLLLFYINEPSVII
                     GRNQNTIEEIDPDYVNTHGVHVVRRLSGGGAVYHDLGNLNFSFVTQGKGDLHQFTKFI
                     QPVVACLRSLGVAAEIRGRSDIFVDGRKVSGNAQYATTARMFSHGTLLFDTDLSAMLR
                     AINPRRITIESKAVQSVRSAVGNLREMLPGNMTIDDLRAAILREIFGAGAVPELRLQD
                     EEWRRIEEISASRYATWDWNYGRSPQFNVQKSGRLPIGKVDARIEVNEGIIRSIRLFG
                     DFSGKRDVAELEAGLVGTPYDRERLAAALTETDLDAYFGRMDVDAFINLIY"
     gene            complement(3182..4345)
                     /locus_tag="PLC06_13030"
     CDS             complement(3182..4345)
                     /locus_tag="PLC06_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRO91416.1"
                     /translation="MTIAQSAVMKSSGLYFGALVVGLTTLLLLVACAPGEAIVATATP
                     EPMVTLPAATAMSTATPIPTTEPTATVEPPPQPTAAPTSTPVPSPTADLSGVGLPCPA
                     VPPARPDYVRYVLSAAPWPTPDPEAEPPPLSLSDPLPAAGRNTGYSYGSDGSGRYLLH
                     NGLDMTDANAAPAGAVADGTVIIARDDLGEMFGWRCDWYGMLVVIRLDMLWNERPVYA
                     LYGHIQDVEVSEGQRVMRGDPVAREGTAGVAVVRHLHLEIRAGENRFGATQNPLLWIE
                     PWSGSGIIAGRLVDPDGRAWQGVTVTLIDRSGESDFLNTWTYLDDPDHLIRPDPSLAE
                     NFVFGPVAAGTYDVYIKLQDVAYRQTVEVKEGQINTVEIVTEPYRTPTPEATP"
BASE COUNT          906 a         1492 c         1322 g          782 t
ORIGIN      
        1 ctgccatctg cgggacgtcg agcgcgaggt gcatcaatcg cggatacgcg ccctgctatt
       61 ggaagacggc gcttttctgc cgggcattga cgccgaccaa tgggcgatcc cgcgaaacta
      121 tcgtggccag gtggggcgtg cggcgctgtc ggggtttttg gccgctcgcg acgagacgat
      181 cgccctgctg tcgccgttat cgcccgaggt gtgggcgcgg caagggcaac actccttctt
      241 cgggccaacc tcgctgcagg aactggtgtt tctggccgtc cagcacgacc gaattcacgc
      301 ccgccagatc gaggcgctcc ggcaagcaag cgacgacaag actgaataga gctgccacgc
      361 ccggcgccta cagctccgta atggactggc gcagggtgat gatcgcctgc gctccggctg
      421 catcctgaag atgggcgtgg tggcgcttgc gataagccaa ctcgctcagc atttgatagc
      481 gttgccccgc cagccggtag gcctcgaccc cctcatcgaa caggagcctc atgcgccggc
      541 ggccccgctg tgccgccgag ctgacgatag ccagctcctc ggccgacaga gtgcccaacc
      601 ccacctcttc ggccagatag tggcgcatcc agcgccgctc ctgatagagc gacaggatga
      661 tgatggccac ggtcaacatc accccgcccc agccaaacgt cagacccgcc aggaaaccgg
      721 cgatggagcc ggtgaacatc gcgacattgt gaaatgcgtg cagagccatc gccgccatca
      781 ggcctaacag cggcgcggcg atgcgcaccg cccaattgcg gctcatccgc gccagggcga
      841 tccccaagcc ggtgagcgac gagtagagcg cgtgattgag gccaaagatg acgccccgca
      901 cgaacaccag ctcgttccag cccaacgccc cgctctcatt gaagtacgtc gtgtagtaga
      961 ggacattctc gaccatggcg aagcccatgc cgaccatcgc gccatagata ataccgtcca
     1021 gcgggctgtc cagctcgttg cgttgaatga taaggatgag gaacagcgcc agccccttga
     1081 caaactcctc gaccactggc gcaatggctc ccccggagat cagttcggcc gtgctttccg
     1141 agagcagcgc gtagaacggc aggctgaaca gcgtgttgaa gaacagagcc agcagagccg
     1201 cggggatggc tccccacacg aaagcggccg acagcagcca taccggctcc ttctcgtaat
     1261 gatccaccca atagatcagg ccgatataga tcagcaccgg caacgcggcc gccagcaccg
     1321 ataccagcac gtaattctca gcgctcaaag aggcacctgt tcgagttaag cggatcccgg
     1381 ccgggaatca tgtacgctaa tatcatggca atatcatggc ccaaccgtga tcaaatcccg
     1441 aatcgcctca aacttcccgt caccaatgcc ggggacatcc atgatgtcct cgatggtggc
     1501 gaaggggcca ttggcctcgc ggtgggcgat gatgttgatc gctgtgccgg ggccaatccc
     1561 cggcagcatc tccaaatcgg tctgggtggc ggtattgata ttgaccagcc cgctgatgct
     1621 gatgccgccc atccggccgg cgactgtcgg ctcggctccc gagggcaagc tgaccggcgg
     1681 tggcgtaggc aacgcttctg ccaataatgg gacatggact tgcatcccgt cggccgccgg
     1741 ctgggccaga ttgacggccg cggtgtcggc gccggcggcg aacccgcccg cggcgcgcac
     1801 ggcgtcgtcg agcaggctgc ccggcggcaa ggtgtaaaca gccggccgca gcaccgcgcc
     1861 gctcacataa atgtggatcg ggccatcggt gggcgaggtt tccgtcatgg gtgcggtcgc
     1921 cggagatgaa gcttgcgtag cttccggcgg cataatctcg attggcgcgg gttgggccgc
     1981 cccgcgcaaa cgcgccaggc caacccccgc tcccaccaga agcaccgccg ccactgccag
     2041 accgaccagt tgccgccaac cgaccatcgc tcgctcctct ccggccgatc ggccggaacc
     2101 cgcatcgcct aatagatcaa attaatgaac gcgtccacat ccatccggcc gaaataggcg
     2161 tccagatccg tctcggtcag cgcggcggcc agccgttcgc gatcataagg cgtgcccacg
     2221 aggccggctt ccagctccgc cacgtcgcgc ttgccgctga agtcgccgaa tagtcgaata
     2281 gaccggatga taccctcatt aacctcgatg cgcgcatcca ccttgccaat cggcagacgg
     2341 ccactcttct gcacgttgaa ctgcggcgag cggccgtaat tccagtccca cgttgcgtag
     2401 cgcgacgcgc tgatctcctc gatccgccgc cactcctcat cttgcagcct aagctcgggc
     2461 acagcgccgg cgccgaaaat ctcgcgcaag atagccgctc tcaggtcatc aatcgtcata
     2521 ttgccgggca gcatctcgcg gagattgcct accgcgctgc gcacggattg aaccgccttc
     2581 gattcgatcg tgattcgccg cgggttgatg gcccgcagca tcgcgctcag gtcggtgtcg
     2641 aacaggagtg tgccgtggct gaacatccgc gcagtcgtgg cgtactgcgc gttgcccgaa
     2701 accttgcgcc catcgacaaa gatatcgctc cggccgcgaa tctcggccgc cacacccagc
     2761 gaacgcaaac aggccacaac cggctggatg aacttggtga actgatgcag gtcgcccttc
     2821 ccctgggtca caaagctaaa attgaggttg cccaaatcat gatagaccgc cccaccgccg
     2881 gagagccgcc gaacaacatg gacgccatgg gtattgacat aatcggggtc gatctcctca
     2941 atcgtgttct gattgcggcc gatgatgacc gacggttcgt taatatagaa cagcaacagt
     3001 ggttccgtct cccgaacatg acgcagcaga tactcctcga atgccagatt caggcgcggg
     3061 tcggtgatgc ccttgttatc gacgaacagc atatatcaat cctatcatgc cggataaaag
     3121 gttgcgacta atagcgatcc tgctccgtgt gttacccatc aaatcgccaa ccccagccgc
     3181 cttatggcgt cgcctccggg gtcggcgtcc ggtatggctc ggtgacgatc tccaccgtgt
     3241 tgatttgccc ttccttgacc tccacagtct gtcgatatgc cacgtcctga agcttgatat
     3301 agacgtcata cgtgccggct gcgaccggcc caaagacgaa gttctcggcc agcgaaggat
     3361 cgggccggat gagatgatca ggatcgtcga gataagtcca cgtattgagg aagtctgatt
     3421 caccgctccg gtcgatgagc gtcaccgtga ccccttgcca ggcgcggccg tcgggatcca
     3481 ccagtcgccc ggcgatgatg ccgctgccgc tccagggttc gatccacaac aacgggtttt
     3541 gcgtcgcgcc gaaacgattc tcaccggcgc gaatctccag atgcaaatga cgcactacgg
     3601 ccaccccggc tgttccctca cgggccaccg gatcgccgcg catcacgcgc tggccctcgc
     3661 tcacctccac atcctggata tggccgtaga gcgcgtaaac cggccgctcg ttccacaaca
     3721 tatcgagccg gatcacaacc agcatcccgt accagtcgca gcgccagccg aacatctcgc
     3781 ccaggtcatc acgagcaatg atgaccgtac cgtcggctac cgcccccgcc ggagcggcgt
     3841 tcgcatcggt catatccaaa ccgttatgga gcagatatcg cccgctgccg tcggagccat
     3901 aggagtagcc ggtgttgcga ccggccgccg gcagcgggtc gctcaaggag agaggtggcg
     3961 gctcggcctc cggatcgggt gtgggccacg gcgcggccga gagaacgtag cgaacatagt
     4021 cgggcctggc cggcggcaca gcagggcagg gcaacccgac cccggacaga tcggccgtcg
     4081 ggctgggcac cggcgtggac gttggcgcgg ccgtcggttg cggcggcggt tcaaccgtgg
     4141 ccgtcggctc cgtcgtcggt atcggcgtgg cagtcgacat ggcggtcgca gccggcagtg
     4201 tcaccatagg ttcgggcgtc gccgttgcca caatggcctc ccccggagca caagccacca
     4261 acaggagcag cgtcgtaagc ccaacgacca aagccccaaa ataaagcccg gaactcttca
     4321 tgacggctga ttgtgctatg gtcattgact tgggtcaacc attctcagga atatgccggc
     4381 aatcataagg caatattgcg cctcggccga tcatccttta tcatgaccgt acacaggtgc
     4441 ccaatgactg acagtctgac gctcgcccag caacacctct acaccttcca ggcctgcccg
     4501 cg
//
LOCUS       DAOMQG010000283         4481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8102_length_4481_cov_11.456846, whole genome shotgun sequence.
ACCESSION   DAOMQG010000283 DAOMQG010000000
VERSION     DAOMQG010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8102_length_4481_cov_11.456846"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1489
                     /gene="zwf"
                     /locus_tag="PLC06_13035"
     CDS             23..1489
                     /gene="zwf"
                     /locus_tag="PLC06_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRO91417.1"
                     /translation="MEEIVSIVIFGASGDLTHRKLIPSLYDIYRKGRLPKEWRIVGMA
                     RSEYTDDEFREYVESGVKEFSPNTYDVGKWQEFARNIVYFQGDISKLDELAALDKRIA
                     EWETDRVGNRIYYLSVGPNLYEVVIGNLGAADMVREDLAWRRVVIEKPFGHDLASAQE
                     LNNSIHQVLAESQIYRIDHYLGKETVQNLLVFRFANSLYEPVWNRNYIDHVQITVAET
                     VDVGHRATFYDGVGVMRDMMQNHVMQLLSLVAAEPPSSFDAEAIRNEKAKVFSAIRPI
                     KPHEVAGCTVRGQYRTYRDAKGVAFGSTTPTFAALRFFIDNWRWQGVPFYMRSGKALA
                     AKVTEISIFFKRPPHVMFPMPEGAKLEPNRLSICIQPDEGIHFSFQAKVPDTAAEMRD
                     VEMSFQYQETFGALAIPEAYERLLLDVIKGDASLFTRSDAIELSWQLIDPILEGWASK
                     HAPPLNFYETGTWGPSEADELIAHNGYHWVHSCGNDEE"
     gene            complement(1504..3852)
                     /locus_tag="PLC06_13040"
     CDS             complement(1504..3852)
                     /locus_tag="PLC06_13040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRO91418.1"
                     /translation="MNDHNDSQPLRLRPAQEEVLAYTGGRMAISAVPGSGKTFTLSLL
                     ATKLIAENQFDPASGRQVLIVTFLNSSVDTFRARIRKRLQEMGLDDTGYDVRTLHSLS
                     LEIVRSAGEIPADELTVLDDLQSRQILEAAVDGWINDNVRLWETFLPESGREYSPQMI
                     ARWRDITATTAASFIRAAKNERYTPAAIQSTIRRRQNNDIDPDESSNVAQSPLMHMMA
                     GIYSRYQSSLDRQGSLDYDDLIWRAADLLEHRPDLVESLRGRWPFILEDEAQDSVPLQ
                     EILLDTLTGPNGNWVRVGDPNQAITSTFTAAHPRFFSKFIDRPDVVAKTLPNSGRSAP
                     LIIGAANAMLNWTIDQHPVPEVRRDAFRRQDILPTPPGDAQPNPPDSEAEMRIRVYRH
                     REDEEIPAIAQLAVEYTRRQPQHTLAILAPTHQTGYAIAQHLDALNADYDNLLRGSGR
                     EREVAAAIHAVLAVLANPLDTKALNAAHNSLSELKESGPIYAVLPEEPEDSARFQALL
                     RSVYRPESILFPREGEPIENLLPTGVASEEDIDRLERLAGFLRDNFDLRTLPIDDLTL
                     ALADVLFAPRSGSNAADDTTHELDLAIAYQIAGQLRQWREAQPEWRLPELVAQLQSVA
                     TGRRTLNVVRDDSGYEPKPGRISLTTQHSGKGLEWDAVFLVGIDGMWIPGSLDAPFLG
                     VHDFLGGDPNAEATAQLSYLMSDEAGIYPNRTATESAHIEVICERLRLLYVGVTRARR
                     FLHISRSRATRRQNREREAEPATVLGVLYRYLQERTGDAPAE"
     gene            3924..>4481
                     /locus_tag="PLC06_13045"
     CDS             3924..>4481
                     /locus_tag="PLC06_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91419.1"
                     /translation="MMFKFVLRHSLFFLLLTGLIALPTGGSYAQGSNPPLEQSAPDDG
                     DVPRFGIVEAFWKPEESAELGVGWDRILFYWNEIQPTGPDDWNTLHVLEEWLDDARAE
                     NREVMGLLKNTPPWATDAEPFSGVPRGLYLPIDDPGNLWAGYVRKVVQYYAPLGVHRW
                     IIWNEPEIEAGVYGHEFSGSTRDYYQ"
BASE COUNT          886 a         1184 c         1382 g         1029 t
ORIGIN      
        1 ttgccctcgt gagagaaaac tgatggaaga aatagtctct attgttattt tcggcgcttc
       61 aggcgacctg acacatcgca agctgattcc ttctctgtat gatatttatc gcaagggacg
      121 tttgcccaag gaatggcgga tcgtcggcat ggctcgtagc gagtataccg atgatgaatt
      181 ccgtgagtat gtggaatccg gcgtcaagga gttctcgccg aacacctatg atgtcgggaa
      241 atggcaggag ttcgccagga acatcgtgta ttttcagggg gatatttcca agctggatga
      301 actcgccgcc ctcgacaagc gcatcgccga atgggaaacc gacagggtgg gaaatcgaat
      361 ttattatttg tcggttgggc ctaatctgta tgaagtggtt attgggaatt taggggcggc
      421 cgatatggtg agggaagatc tcgcctggcg acgggtagtg atcgagaagc cattcggtca
      481 cgatcttgca tccgcgcagg aattgaacaa ttccattcac caggtgctgg ctgagtccca
      541 gatttaccgg atcgatcatt atttgggcaa ggaaacggtt cagaacctgc tggtgtttcg
      601 tttcgccaat tcgctttatg agccggtctg gaatcgaaac tacatcgacc acgtgcagat
      661 cacggttgcc gaaacggtgg atgtaggtca ccgggccact ttctacgatg gcgtgggcgt
      721 tatgcgggac atgatgcaaa accacgtgat gcaacttctc tcccttgttg ccgccgagcc
      781 gccgtcttca ttcgacgccg aagcgatccg taatgagaag gccaaagtgt tcagcgccat
      841 ccggccgatt aagccacacg aggttgccgg atgtacggtg cgtgggcaat atcgcactta
      901 tcgggacgcc aaaggtgttg ctttcggctc gaccacacct actttcgcgg ctttgcgatt
      961 ctttatcgat aactggcgct ggcaaggggt tccgttctac atgcgctcag gtaaggcgct
     1021 ggcggcaaag gtgacggaga tctccatctt tttcaagcgg ccgccccatg tgatgttccc
     1081 tatgcctgag ggcgccaagc ttgagcctaa ccggttgtcg atttgcatcc agccggacga
     1141 gggtatccat ttctcttttc aggctaaagt gcccgatacg gcggccgaga tgcgcgacgt
     1201 cgagatgtcg tttcaatatc aggagacatt cggcgcgctg gcaattcctg aagcgtacga
     1261 gcgcctatta ctggatgtca tcaagggtga tgcatcgctc ttcacccgca gtgacgcgat
     1321 agagctttcg tggcaattaa tcgatcccat tctggaagga tgggcgtcga agcacgcccc
     1381 gccattgaat ttctatgaga cgggcacctg ggggccatcg gaagccgacg agctaattgc
     1441 ccacaatggt taccattggg tccacagctg cggcaacgac gaggaatagg gtttatcggc
     1501 cgatcactca gccggagcat ccccggtacg ttcctgcaga tagcgataga gcacgcccag
     1561 aaccgtcgcc ggctcagcct cgcgctcgcg attctggcgg cgcgtggcgc ggctgcggct
     1621 gatgtggaga aaacggcgcg cccgcgtgac ccccacatag agcaagcgca accgctcgca
     1681 gatgacttcg atatgagccg attcggtcgc ggtccggttg ggatagatcc ccgcctcgtc
     1741 gctcatcaga taggacaatt gcgcggtggc ttcggcatta ggatcgccac ccaggaagtc
     1801 gtggacgccc aggaacggcg cgtccagact gccggggatc cacatgccgt cgatacccac
     1861 gagaaaaacg gcgtcccatt ccaggccctt gccgctgtgc tgcgtggtca gcgagatgcg
     1921 gccgggcttg ggttcgtatc ccgagtcatc gcgcacgaca ttcagcgttc ttcgcccggt
     1981 agccacggac tggagttggg ccaccagttc gggcaggcgc cattcgggtt gcgcctcgcg
     2041 ccattggcgc aactgaccgg ctatttggta ggcgatggcc aggtcaagct catgagtcgt
     2101 gtcatcagcg gcgttgctgc cggaacgcgg cgcgaacagg acatcagcca aagccagcgt
     2161 caggtcgtcg atgggcagcg tgcgtagatc gaaattatcg cgcagaaatc cagccaatct
     2221 ttccaggcgg tcgatatctt cttccgaggc cactccggtg ggtaacagat tttcaatcgg
     2281 ctcgccttcg cgagggaaga ggatcgactc gggacgatag acgctgcgca gcagtgcctg
     2341 gaaccgggcc gaatcttccg gctcctccgg cagtactgcg taaatcggcc cggactcttt
     2401 gagttcggaa aggctgttat gggcggcatt cagggctttg gtatcaagcg ggttcgccaa
     2461 tacggctaaa acggcatgga ttgcagcggc tacttcacgc tcgcggccgc taccgcgcaa
     2521 caagttgtcg taatcggcgt ttagagcgtc gagatgttga gcgatggcgt aacctgtctg
     2581 atgggtcgga gccaggatag cgagggtgtg ttgcggctgt cgacgagtgt attccacggc
     2641 cagctgggcg atggccggta tctcttcgtc ttcgcgatga cgatacacac gaatgcgcat
     2701 ctccgcctcg ctgtcaggcg ggttcggctg cgcatctccc ggcggagtcg gcaggatgtc
     2761 ctgtcgccga aaggcgtctc gcctcacttc cggcacggga tgctggtcga ttgtccagtt
     2821 gagcatcgca ttggccgcgc cgatgatcag gggggccgag cgcccgctgt ttggcaaggt
     2881 tttggcgaca acatcaggtc ggtcgatgaa cttcgagaag aagcgcggat gggcggcggt
     2941 gaaggtgctg gtgatggcct ggtttgggtc gccgacgcgt acccaattgc cgtttggccc
     3001 ggtcagggtg tccagcagaa tctcttggag tggcacgctg tcctgggctt cgtcttcgag
     3061 tatgaacggc cagcgaccac gcaggctttc gacgagatcc ggccgatgct cgagcagatc
     3121 agccgcgcgc cagatcaggt cgtcgtagtc caggctgccc tggcggtcga gggatgactg
     3181 gtagcgacta tatattccgg ccatcatgtg catcaagggt gattgggcga cgttagagct
     3241 ttcgtcggga tcgatgtcgt tgttttgccg gcggcgaatt gtgctctgga tggccgcggg
     3301 agtatagcgt tcgttcttgg cggcacggat gaatgaagcg gccgtcgtgg ctgtgatgtc
     3361 tcgccagcga gcgatcattt gcgggctata ctctcgtccc gattcgggca aaaatgtctc
     3421 ccacaggcgc acattgtcgt taatccagcc gtcgacggcc gcctccagga tctgtctgct
     3481 ttgcagatca tcgagcacgg tcaattcgtc ggccgggatt tctccggccg atctgacgat
     3541 ctccagcgag aggctatgca gtgtgcgaac gtcgtagccc gtatcatcca gccccatttc
     3601 ctgcaagcgt ttgcgaattc gggcacggaa ggtgtcaacg ctactattaa ggaacgtgac
     3661 gatgaggact tgtctgcctg atgccgggtc gaactgattt tcggcaatta gcttggttgc
     3721 cagcagcgag agcgtgaaag tttttccgct gcccggcacg gcgctgatgg ccattcggcc
     3781 gccggtgtag gccaggactt cttcctgggc aggtcgtagc cttaacggtt gggaatcgtt
     3841 gtggtcgttc attggagaag attaccccag acattataat caaaactttg cggaccgaca
     3901 tttccggatt acttgcattg cttatgatgt ttaagtttgt cttgcgacac tcgttgtttt
     3961 ttttgctgct gacaggttta atcgctcttc caaccggtgg gagttacgct cagggaagca
     4021 atcctccatt ggagcaatct gcccctgatg atggggatgt tccccggttt ggcattgtcg
     4081 aggctttttg gaagccggaa gaatcggccg agttgggagt aggctgggat cgcattctct
     4141 tctattggaa tgagatacaa ccgaccggcc ccgatgactg gaacacactc cacgttctgg
     4201 aagaatggct ggatgatgcc agggcagaga atcgggaggt gatgggtctt ctcaagaaca
     4261 cccctccctg ggccaccgac gccgagcctt tctccggcgt gccgcggggg ctttacttgc
     4321 cgatagatga tccggggaat ttgtgggccg ggtacgtgcg caaggtcgtt caatattacg
     4381 cgccgctggg cgttcatcgc tggattattt ggaacgagcc ggagatcgaa gccggtgtct
     4441 atggccatga gttcagcggc tccacgcgtg attattatca g
//
LOCUS       DAOMQG010000284         4467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8139_length_4467_cov_13.262466, whole genome shotgun sequence.
ACCESSION   DAOMQG010000284 DAOMQG010000000
VERSION     DAOMQG010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8139_length_4467_cov_13.262466"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLC06_13050"
     CDS             <1..555
                     /locus_tag="PLC06_13050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRO91420.1"
                     /translation="FAGNITLDHGAYLDESVYLHACPGGIHIGARTIVMYGAVLHVYN
                     FRGMPHSRITIGQDSLIGEYTVIRGQGGVTIGSRVYTSPFSQIIAVNHVFSDPDQSFT
                     RQGITAEGIIIEDDVWIGSGAIITDGVRVGRGSVVAAGAVVTCDVPPHAVVGGVPARV
                     LKMIDIQGDAGNNRTVYHEPGNAR"
     gene            598..1821
                     /locus_tag="PLC06_13055"
     CDS             598..1821
                     /locus_tag="PLC06_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO91421.1"
                     /translation="MQTTSLTVVIPAYNEESGIAQIASRVLATRDELAAIGVSEFELL
                     IVDDGSHDDTARIAAGIPGVNLIRHPTNKGYGAALKTGFNQSQADLIGFLDADGTYPP
                     EYFSRLCAVAMDGADLVIGSRMAGADSKMPVTRRIGNIFFARLLSLVGRQTITDSASG
                     MRVFRRDILDCLYPLPDGLNLTPVMSTRAVHEGIRMAEVPIPYSERLGRSKLSVVNDG
                     SLFLRSMLWTALQYNPVRILGMLGIAGVLIGLLCVAVLVIMRLSGVTVLTPWTVVALY
                     TGVVAGMVGVNLFLLGIVFNYLIALFRGRPVQQGLFGRKFFDSPLDRHFGWMGIGSFL
                     AGLVIAGVAVILGIGGWDMGQLWLYGLASAMLILLGVELVTFWVIFRVLDELNTQAMR
                     ESRSINQGRERDFSA"
     gene            1866..3536
                     /locus_tag="PLC06_13060"
     CDS             1866..3536
                     /locus_tag="PLC06_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRO91422.1"
                     /translation="MPKIEDKNLVTENLGSRTAPKLRMADFPSADEIVQRNLNASVRS
                     EGTVDILFVNPPTPDGGLWIRSQHRVGRRTRENMVWPQVSLAQLAAMVYPQHSVAIID
                     ANAERMSWTEFTHKLEGFRPRYYLTQVTAPTLENDMYGCFLAKARGAKTIAFGTHVTP
                     IPRETMRAYPALDFVLIGEPDLTIRDLLDHLEGKIEQRPSNIQTMFEKHDPLYRPAIT
                     DNGNIDMNGIKGVGWRKGEEIIINPTRPFLRDLDDMPHPLHHLLPLNTYRMPMIHGPF
                     TFIVSSRGCPAGCTYCIKHVSYQFTTRLRSPEHIMEELWILKRLGMNNIHMYADLFTV
                     SREQVMKLCELMIQENLQIRWTCNSRVDYVDEEMLQMMAKAGNWLISWGIESGNDQIL
                     RHARKGAYTEKAERSLLWSRKADIRNWGYFIIGLPGETEETIRQTIEFSKRLPLDIAL
                     FHVAAPYPGTPFFFEVVENGWFRPGTRWEQVDMDKGTVLDYPGLSAERLLYWQKRAFR
                     EWAMRPGPIMTYLKMLVSDPSTLRSALNVGIQHLSWVSTDTGSPAGAA"
     gene            3704..>4467
                     /locus_tag="PLC06_13065"
     CDS             3704..>4467
                     /locus_tag="PLC06_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91423.1"
                     /translation="MIAGAPWAARVISGHSPVVRTRFDPFLLMFVTGAAIGVWAAYDQ
                     PAAIEKFWLILGGVILFYALAGQRAANIWPLLLGVAFFGGGVGLYFLLTHNWNEAPAK
                     IDSFNQLGLQLMNLRPAIFQNLHQLHPNVAGGIIALLFPCAIAAMVRMLRERRFLLAL
                     LVAAALGIMILALALTTSRGAWIALFGGLVAWLIWIGAKDMSNSLFFSRRKTLGLAGL
                     VLSGIALSVVLLSPRGLSGFFDRLPGPASAGSRMQLA"
BASE COUNT          883 a         1137 c         1308 g         1139 t
ORIGIN      
        1 tttgccggca atattaccct ggatcatggt gcttacctgg atgagtctgt ttatttgcat
       61 gcctgtcctg ggggtatcca tattggggcg cgaaccattg tgatgtatgg tgccgtgctc
      121 catgtttata acttccgggg aatgccccat tcacgaataa cgattggtca ggacagcctt
      181 attggagagt atactgttat ccgtggccag ggcggggtca ccatcggcag cagggtctac
      241 acttcgcctt tttcgcagat tatcgctgtc aatcacgtgt tttctgatcc tgatcaatcc
      301 ttcacccggc agggcatcac agcagaaggg atcatcattg aggatgatgt ctggattgga
      361 tccggggcga tcattactga tggcgttcgt gttggccgtg ggtcggttgt ggctgctggg
      421 gcagtcgtga cctgtgatgt tcctccccac gctgttgtag gcggtgtccc ggctcgtgtg
      481 ctgaagatga tcgatattca gggtgatgcg ggaaataaca gaacagttta ccacgagccc
      541 ggtaatgctc gctgagcgtt cctgttggtc atggtttgtt caaggatgtg tccctaaatg
      601 caaacgacct cgctaacagt cgtgataccc gcctataacg aagaatcagg cattgcccaa
      661 atcgcgagcc gcgtcctggc cacacgggac gaactggcgg ctatcggtgt gagtgaattt
      721 gaactgttaa ttgtcgatga cggctcacac gacgacactg cgcggattgc ggccgggata
      781 cccggcgtta acctcattcg gcatccgacc aacaagggat atggtgcggc gctaaaaacc
      841 ggcttcaacc aatctcaggc tgacctgatt ggcttcctcg atgcggatgg aacttatccg
      901 ccggaatact tttcccgatt gtgcgcggta gcgatggatg gggctgatct ggtgataggt
      961 tcccggatgg ccggcgctga cagcaagatg ccggtgacgc gacgtattgg gaacatattt
     1021 tttgcccggt tgttgtcgct cgtcggccgc cagacgatta ccgatagcgc gagcggaatg
     1081 cgtgtctttc ggcgggatat cctggactgc ctctatcctc tgcccgatgg tctaaatctg
     1141 actcccgtga tgagcacacg tgccgtccat gaaggcatac gcatggctga agtcccgatc
     1201 ccatactccg aacgtttggg gcggtcgaag cttagtgtcg tcaatgacgg ttcgctattc
     1261 ctgcgatcca tgctctggac ggcgttgcaa tataatccgg tacgtattct ggggatgctt
     1321 gggatcgccg gtgtattgat tgggttgttg tgtgtagctg ttttggtcat catgcgcctg
     1381 tccggcgtta cggtcctcac accctggacg gtggtcgcgc tctataccgg cgttgtggcc
     1441 ggcatggtgg gtgtcaatct atttctattg gggatcgtgt tcaattatct gatcgcgttg
     1501 tttcggggca ggccggtgca gcaagggcta ttcggtcgga agtttttcga cagccctctt
     1561 gacaggcatt tcggctggat gggaataggt tcgtttctgg caggtttggt aatcgccggt
     1621 gttgcagtta ttttaggaat tggtggatgg gatatgggtc agttatggct ttacggcttg
     1681 gcgagtgcga tgctaatcct gcttggtgta gagcttgtaa ccttctgggt catattcagg
     1741 gtcctggatg agttgaatac acaggctatg cgcgaaagcc ggtcgataaa ccaagggcgg
     1801 gaacgagatt tttcggcgtg aacagccaag actgtttgtg ctggagaatg gagacaacaa
     1861 gggctatgcc aaaaatcgaa gataaaaatc ttgtaacgga aaacctcggc tctcgcacag
     1921 cgcccaagct gcgtatggcc gactttccaa gcgccgacga gatcgttcag cgaaatttga
     1981 acgcttcggt gcgatccgaa gggacggtcg atattctgtt tgtgaacccc cccacgcctg
     2041 atggcggctt gtggattcgc agtcagcatc gcgtcggccg ccgcacgcga gagaacatgg
     2101 tctggcctca agtctctctg gcacaactcg cggccatggt ttacccacag cattcggtgg
     2161 ccatcatcga cgctaatgcc gagcgcatga gttggactga gtttacccat aagctggaag
     2221 gattcagacc ccgctattat ctgacccaag tgacggcccc aacgctcgaa aacgacatgt
     2281 atggttgctt tctggcgaaa gcgcgtgggg cgaaaaccat cgcctttggg actcatgtta
     2341 caccgattcc acgtgagacg atgcgtgcct atccggcgct cgattttgta ttaattggcg
     2401 aacctgatct gactatccgt gatttgctcg accatttaga ggggaagatt gaacaacgcc
     2461 cgtcgaatat ccaaacgatg ttcgaaaagc acgatccgct ttaccgacct gccattaccg
     2521 acaacggcaa cattgacatg aatggcatta aaggagtggg ctggcgcaag ggagaggaga
     2581 tcatcatcaa ccctacgcgg ccgttcctgc gcgatctcga cgacatgccc catccactcc
     2641 atcatctgct gcctctcaac acgtatcgca tgccgatgat ccatggtccc tttacgttca
     2701 tcgtctccag ccgtggctgc ccggccggat gtacttattg catcaagcat gtcagttacc
     2761 agttcacaac gcggcttcgc tctcctgagc atattatgga ggagttgtgg attctgaaac
     2821 gtcttggcat gaataatatc catatgtacg ccgacttgtt taccgtcagc cgggagcagg
     2881 tcatgaagct gtgcgaattg atgatccagg agaacctcca gatccgctgg acttgtaaca
     2941 gtcgtgtgga ttacgtcgat gaagagatgc tgcagatgat ggccaaagcg ggcaattggc
     3001 ttatctcctg gggcatcgaa agcggtaacg atcagatctt gcgccatgca cgaaagggag
     3061 cgtatactga gaaggctgag cgctctttgc tctggtcacg caaggccgat atccgcaact
     3121 ggggttactt cattattggc ttgccgggcg agacggaaga aacaattcgg cagacgatcg
     3181 agttttcaaa gcgcttgccc ttggatattg ctttgttcca cgttgctgcg ccatatcccg
     3241 gcacgccatt cttctttgaa gttgtggaga atggttggtt tcgtccgggc acgcgttggg
     3301 aacaggtaga catggataag ggaacggtac tggattaccc aggcctgtcg gccgagcggc
     3361 tgctctactg gcagaaacgc gccttccgcg aatgggctat gcgtccgggt ccaataatga
     3421 cttatctgaa aatgcttgtg tccgatccct caacccttcg ttctgcattg aatgtgggga
     3481 tccagcattt gagctgggtc tctaccgaca ccggatcgcc cgccggcgcg gcataatggg
     3541 tagaacgaat aacgaagtaa tgatggcggc cgatgagtcc cccggcaggc tgcggtcgac
     3601 attcgtgaac agtcaccgtt ttgcctggat tcacctgggt tgtgccttgg tggccgcggc
     3661 gatctggtat gttagcggcg gccgagccgg gccatggccg ctgttgattg ccggagcgcc
     3721 gtgggcagcc cgggtgatca gtgggcactc cccggttgta cgaacacggt tcgatccttt
     3781 cctcctgatg ttcgtaacag gcgcggcaat cggtgtttgg gctgcctatg atcagccggc
     3841 cgcgattgag aagttttggc tgatccttgg cggtgtgatt ctcttctatg cgctcgccgg
     3901 ccaacgcgca gccaacatct ggccattgct gctcggcgtt gccttctttg gcggcggcgt
     3961 ggggctttat ttcctgctga cacataattg gaacgaagct ccggctaaaa ttgactcgtt
     4021 caatcagcta ggcctgcaat taatgaacct tcgcccggct atctttcaaa acctgcatca
     4081 gcttcatccc aatgtcgccg gcggaattat cgctctcttg tttccctgtg cgattgcggc
     4141 gatggtgcgg atgctgcgcg agcgacgttt tttgttggcc ctgctcgtgg ccgctgcgct
     4201 cggcattatg atcctggcgt tggctctgac gacctcgcgt ggcgcctgga tagcgctctt
     4261 cggcgggttg gtagcctggt tgatatggat tggcgcgaag gatatgtcca actccctctt
     4321 tttttccagg cgcaaaaccc tggggttggc cggattggtg ctgtcaggga ttgcgttatc
     4381 ggtggtgttg ctttctcctc gcggactgtc gggattcttc gatcgccttc ctgggccggc
     4441 tagtgcgggg agtcggatgc aactcgc
//
LOCUS       DAOMQG010000285         4467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8140_length_4467_cov_7.382366, whole genome shotgun sequence.
ACCESSION   DAOMQG010000285 DAOMQG010000000
VERSION     DAOMQG010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8140_length_4467_cov_7.382366"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1728
                     /locus_tag="PLC06_13070"
     CDS             124..1728
                     /locus_tag="PLC06_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRO91424.1"
                     /translation="MGVINMVSSSLPALRDRFVTLEQFLPLEVVTGSRLATAFAGFAL
                     LILSVNLWRRKQVAWFLTLLALAVSALSHLLKGLDYEEASVAVALIVILFIFRSRFHA
                     LSDFPSIRRGVLILAAALGFTLIYGAVGFFLLDRHFHEKFGLPAALRQTLFMFVEFSD
                     PGPEPTTRFGRYFANSIYFIAAATIGYSLFMLVRPVIIRRPASRDERERARKIVEQYG
                     RSSIARFTLFDDKSYFFSPNGSVIAYVVKGGIALALGDPIGPEEDAGAAITTFGDYCA
                     HNDWRSCYYQVRPDYLTLYNANGYRALNIGQEGTVDLKTFSLEGKANKDFRTVKNRMT
                     RLGFTTELIEPPLSDDILGELRAISDAWLDMMHGSEMKFSLGWFDDDYIRNSPIMVVR
                     DPTGQITAFANFVSEYRRNELTLDLMRRLGEVESGTMDYLFVNIIEWAQQKGFATFSL
                     GLSALAGVGETAEDPALEKALHMIYENADRIYHFKGLHNFKSKFHPQWEPRYLIYRNV
                     STLPAIATALARAHSGDNFIWAYLNK"
     gene            1792..3177
                     /locus_tag="PLC06_13075"
     CDS             1792..3177
                     /locus_tag="PLC06_13075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO91425.1"
                     /translation="MLPTKTDIIICGAGIAGISAAWHLAARHGISDILLIEEGSPLML
                     TSDKSTEAYRNWWPGPGDAMVRFMNRSIDLLEELAALSDNYFAMNRRGYVFLTADPST
                     AERYRLSAEESQRLGAGELRVHRGRPDDPSYPAHHAEGWPPELVGADLVLDPAIIRER
                     FPFISDDAVAMLHPRRCGWLSAQQLGMWLLSEAKARGVRLLNGRVTAVDTTSGRVNGV
                     TVRTGDGEQRIETRVFVNAAGPLVDKVGALLGVEIPVFNELHGKVAINDPLGIVPRDA
                     PLMIWSDPVTLPWDDEEREALAADEETRWLTEPFPAGVHFRPEGGPGATTLLLLWTYH
                     VEPTPAVWPVRFDPEYAEVVVRGLTRMIPGLAAYAGNFGRPYIDGGYYCKTRENRPLI
                     GPLPVAGAYIFGALSGYGIMASQAGADLLAAHITGGALPDYAAAMGLSRYEDAAYRAL
                     LEGWDAGAGQL"
     gene            3397..4023
                     /locus_tag="PLC06_13080"
     CDS             3397..4023
                     /locus_tag="PLC06_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91426.1"
                     /translation="MSDVTRKPALDNISIEFLLKEYERLSEYQKASFDMYHRRFDIYL
                     AAVSGTLLIAVALSQTTQSPFQQTAITTLLVVLLVIGLNVFASLSYSNASQVHLERAT
                     RLIQSCFIERDAGINGFLYFDKHRSSISGTDLRTLLIRGITGGGPKSILVIVNSLLVS
                     ILLIRFVKLVGEIDLPFPVQVLVGVGAFLLCSILHVVYARWIYRINGI"
     gene            complement(4265..>4467)
                     /locus_tag="PLC06_13085"
     CDS             complement(4265..>4467)
                     /locus_tag="PLC06_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRO91427.1"
                     /translation="LLPDPDIETSLSIMESYDPYVVPHREINRHFLTHPSNGILARHT
                     IYWRGLTPDQTADAVLAAVSLR"
BASE COUNT          890 a         1275 c         1296 g         1006 t
ORIGIN      
        1 gttccctgta cttctgcgca agttctaaat aacaagatgc aaacagaacc ccaggtcgcc
       61 cattcgataa aacggccctt agacatccat tctgcccatc tcatcgcttt actgatcgcg
      121 ttaatgggtg tgataaacat ggtgtcgtct tccctacccg ccttgaggga tcgcttcgtg
      181 acccttgaac agttcctgcc tctggaagtg gtcacgggca gccggctggc caccgcgttt
      241 gccgggttcg ccttgttgat cctgtcggtg aacctgtggc gacgtaaaca agtcgcctgg
      301 ttcctgactc tgttggccct ggcggtgtcg gcattgagcc acctgctgaa ggggctggat
      361 tatgaagaag caagcgtcgc cgtcgccctg atcgtcatcc tgtttatctt tcgctcccgc
      421 tttcatgccc tgtctgactt cccctcgatc cggcgaggcg tcctcatcct ggcggcggcc
      481 ctgggattta cactcatcta tggagccgtg gggtttttcc tgctggatcg acatttccac
      541 gaaaagttcg gcctgccggc cgcgctccgg caaaccctgt tcatgttcgt cgaattttca
      601 gaccccgggc cggagccaac gacccgattc ggccgatact tcgccaattc aatctacttc
      661 atagccgccg ctaccatagg ctatagcctg ttcatgctcg ttcggccggt gatcatccgc
      721 cgaccggcga gccgtgatga gcgcgagcga gcgcggaaga tcgtcgagca gtatggacgc
      781 tcgtccatag cgcgcttcac gctcttcgac gataaatcct acttcttcag ccccaacggg
      841 tcggttatcg cctatgtcgt taaaggggga atcgccttgg cgctcggcga tccgatcggc
      901 ccggaagagg atgccggagc agcgatcacc accttcggcg attattgcgc ccacaacgac
      961 tggcgatcct gctattacca ggtccgaccg gactatttga ccctgtataa tgcgaacggc
     1021 taccgcgccc tcaacattgg gcaggaaggg accgtcgatt tgaagacgtt ctctctcgaa
     1081 ggcaaggcaa ataaggattt ccgcaccgtc aagaatcgca tgactcggct cgggttcacg
     1141 acggaattga tcgagccgcc cttgagcgac gatattcttg gcgaattgag ggccatcagc
     1201 gacgcctggc tggatatgat gcacggcagc gaaatgaagt tttcgcttgg ctggtttgat
     1261 gacgactata ttcgcaacag ccccatcatg gttgttcgcg atcctaccgg gcagatcacc
     1321 gcttttgcca atttcgtgtc ggaataccgg cgcaatgagt tgacgcttga cctaatgcgg
     1381 cgtcttggcg aggttgagtc gggcacgatg gattaccttt tcgtgaacat catcgaatgg
     1441 gcccagcaga aggggtttgc cactttcagt ctgggcctga gcgcgctggc cggcgtgggc
     1501 gaaacggccg aggacccggc ccttgaaaag gccttgcaca tgatctatga aaacgccgac
     1561 aggatttatc atttcaaggg cctgcacaat ttcaagagca agtttcatcc ccaatgggag
     1621 cctcgctatt tgatctaccg gaatgtctcc accctgccgg ccatcgccac ggcgctcgcc
     1681 cgcgcccata gcggcgacaa tttcatttgg gcctatctca ataaataacc ggcaagcccg
     1741 gccgagcgac cgtatcttgc gttcgcgcgt ctttgcgcta caactaccgt catgctccca
     1801 accaagaccg acatcatcat ttgtggcgcc ggcatcgccg gcatctcggc cgcctggcat
     1861 ctggcggcgc gtcacggcat cagcgacatc ctgctcatcg aggaaggctc gccgctgatg
     1921 ctgaccagcg acaaatcgac cgaggcctat cgcaactggt ggcccggccc cggcgacgcg
     1981 atggtgcgct tcatgaaccg cagcattgac ctgctggaag agctggccgc cctgagcgac
     2041 aactatttcg ccatgaaccg gcgcggctat gtctttctga cggccgatcc gtccacggcc
     2101 gaacgctatc gcctgtcggc cgaggaatca cagcgattgg gcgcgggcga gttgcgcgtc
     2161 catcgcggcc gccccgatga tccctcctat ccggcccacc atgccgaagg ctggccgccc
     2221 gaactggtag gggccgacct cgtgctcgac ccggccatta ttcgcgagcg cttcccgttc
     2281 atcagcgacg acgccgtcgc catgctccat ccgcgccgct gcggctggct cagcgcgcag
     2341 cagttgggca tgtggctgct gagcgaggcc aaggcgcgcg gcgtgcggct gctcaacggc
     2401 cgcgtgacgg ccgtggacac gaccagcggc cgggtcaacg gcgtgacggt gcgcaccggc
     2461 gacggcgaac agcgcatcga aacccgcgtt ttcgtcaacg ccgccgggcc gctggtggac
     2521 aaggtggggg cgctgctcgg cgtggagatt ccggtcttca atgagctgca cggcaaggtg
     2581 gccatcaacg acccgctggg catcgtcccc cgcgacgcgc cgctgatgat ctggagcgac
     2641 ccggtgacgc tgccgtggga cgacgaggag cgcgaggcgc tggcggccga cgaggagacc
     2701 cgttggctga ccgagccgtt cccggcgggc gtccacttcc ggcccgaagg cggccccggc
     2761 gcgacgacgc tgctgctgct gtggacctac catgtggagc cgacgccggc cgtctggccc
     2821 gtccgcttcg acccggagta tgccgaggtg gtggtgcgcg ggctgacgcg gatgatcccc
     2881 gggctggcgg cctatgcggg caacttcggc cgcccctaca tcgacggcgg ttactactgc
     2941 aagacgcgcg agaatcggcc gctcatcggc ccgctgccgg tggcgggggc ctacatcttc
     3001 ggcgcgctgt cgggctacgg catcatggcc tcgcaggcgg gggcagactt gctggcggcg
     3061 catattaccg ggggcgcgtt gccggattac gcggccgcga tggggttgag ccgctatgag
     3121 gatgcggcgt atcgggcgtt gctggagggg tgggatgcgg gggcggggca gttgtgagag
     3181 ttgtaactca agccgcgaat atggaataat ctaattgcaa tctgcgggta gattagagct
     3241 atgattatca ggcgtggctg atgcctgttg tgctattgac caagtgacta tttgccacga
     3301 caattccatt tgggattggt actaagttgg ggatgatttc ctgtctatcg gtagcattgg
     3361 cacttcaagt atgtagcaga taggaggagc aatctggtgt cagacgttac tagaaaacca
     3421 gcgctcgata atattagtat tgaattcctg ctcaaagagt acgaacgtct ttctgaatac
     3481 caaaaggctt cctttgacat gtatcataga agatttgata tctatttggc agcggtttca
     3541 ggaacactac tgattgcagt tgcactatca caaacaactc aatctccatt tcagcagact
     3601 gccataacca ctcttttagt agtacttcta gttattggcc ttaacgtctt tgcatccctc
     3661 agctattcaa acgcttctca agttcatttg gagcgggcaa caagattgat ccaaagttgc
     3721 ttcatagaac gcgacgctgg aatcaatgga tttctctatt ttgacaaaca tcgaagctcg
     3781 atctctggca ctgacctccg taccttatta atcagaggca taacaggagg cggccctaag
     3841 tcaattttag ttatagtgaa tagccttctg gtatccatac tcctgataag atttgtgaaa
     3901 ttggtaggtg aaatcgatct tccgtttcct gtccaggttc tggttggtgt gggtgctttc
     3961 ctattgtgtt caattctgca tgtcgtttat gcaaggtgga tctatcggat aaatggaata
     4021 tgattcattt aacaagacga ggcaatcaca aagggcgcct ttattgtccc atccagccta
     4081 aaacccacat ccagtatgga ttgtaatctt gggtactaaa ttgactggta ttacctgtat
     4141 tcttaatctt cttccttaac actgttctga aacaagttaa cggtatcgat ggggcggcga
     4201 cccatgttgc ggtagccacg gtggggacct gcggatttgc catcgtccag cagcgaacca
     4261 gttcctaccg gagcgagacg gccgccagta cagcgtcggc ggtctgatcc ggcgtcaggc
     4321 cgcgccaata gatggtgtgc cgggccagta tgccattgga gggatgggtc aggaagtggc
     4381 gattgatctc ccggtgaggc acgacgtagg ggtcatagct ctccatgatg gacagggaag
     4441 tttcaatgtc cgggtcgggc agcaaca
//
LOCUS       DAOMQG010000286         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8154_length_4459_cov_8.551317, whole genome shotgun sequence.
ACCESSION   DAOMQG010000286 DAOMQG010000000
VERSION     DAOMQG010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_4459_cov_8.551317"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PLC06_13090"
     CDS             complement(<1..1346)
                     /locus_tag="PLC06_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91428.1"
                     /translation="MTTPLPDSVLLERRYQGERPLRTLLSLYKPERGRMLVAAVFFII
                     KHSPVWMLPALTANIIDIISTPGSDLSGLWFNALLLVLLLVQNVPLHTLYARYLSRAV
                     RETETRLRAAICRRLQHLSISYYTRQSAASLQTKVLRDVENIEQMTRIFYDSGLSAVS
                     NIVFALAITAWRVPQFLLFYVLVVPVAALLVRGLRQTLAERNRAFRQELETLSTRVEE
                     MTHLIPITRAHGLEEDALERVNETLYRVRESGTQLDSVNAVFGALSWMTFNLFNVLCL
                     VAAAWAYWTQAIPLTIGEVVMLTSYFSTLTNAVMVLANMTPQISKGLESIRSIGEVLE
                     NPDLEQNEGKAAVESVIGEITFEDVGYAYPESAANAIRDFSLSVTAGETVALVGPSGA
                     GKSTVLNLVIGFIRPTTGRLLLDGRDMQTLDLRTYRRFLSVVPQESILFDGSIRDNV"
     gene            complement(1395..4391)
                     /gene="glyS"
                     /locus_tag="PLC06_13095"
     CDS             complement(1395..4391)
                     /gene="glyS"
                     /locus_tag="PLC06_13095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRO91429.1"
                     /translation="MSSDKLNFQQMILRLLDYWQAQGCLVWQPYNVQVGAGTMNPATI
                     LRVLGPEPWNVAYVEPSIRPDDGRFGDNPNRMQQHYQLQVILKPDPGNPIELYLGSLE
                     AIGISRREHDIRFVEDNWQSPALGAWGLGWEVWLDGQEISQYTYFQQAGGIDLDPVSV
                     EITYGLDRIALALQGKNSVWEMDYGAGVKYGDALLQAEIEHCNYYFNVADVDALKMIY
                     DTYEREALRCLEAGLVAPAHDYNLKCSQLFNVLDTRGAVGVTERAQYFGRMRRIAHKI
                     SLAYMEQRQRLEYPFAENKVWTTDHRPRTTNDIGGLSSVVRGLPEDGDPFVLEIGSEE
                     LPHDDLTSALAQLRAAVPALLDAARLSYERVEIDGTPRRLAVIVHGLADRGADVESVV
                     KGPPADRAFDAGGKPTPAAEGFARSRGLSVDDLSVVEEGGKRYVSAVVHETGRPSLEV
                     LAERLPDLIAGIKFEKSMRWNASNVSFSRPLRWLVALHGPAVVEFEYAGVRSGRTTFG
                     LRPYGSPAWEIRHAADYRTIVDNATIVLHTDARRKAIAAGAHELARSVGGVIPDDPAL
                     LDEVTNLVERPTPLLGSFEERFLALPPMVLVAVMRKHQRYFPVYKGDKLLPYFIAVRN
                     GDEEHLDIVRDGNEHVIRARFADAAFFYEKDIRQPLAAFVPELGKLTFQTELGSFLDK
                     THRLEQLVPVVAGMLGLDEAARATAARATSLAKADLATSMVVEMTSLQGKMGGHYARR
                     GGESPEVAAAIAGQYEAVSDSPAGMALAVADRLDSLAGLFAAGLGPKGSNDPFALRRA
                     ALHLIENLTANRQPFDLRAGLDAAGALLPVAWSDKTRAEVLAFIGGRLDVVLRDAGYS
                     ASVVKATLAEQTHDPYAATRAAAALAEAIQSPDWETLLNAYARCVRITRPLKERYTLR
                     PDDLRLPEEQALAAALVEAEAAKDGTVETFVASLRRIEPAITRLFDAVLIMDDDAAVR
                     ENRLALLQRVAGLARGVADLSMLEGF"
BASE COUNT          798 a         1452 c         1474 g          735 t
ORIGIN      
        1 acattgtcgc ggatggagcc gtcgaacagg atcgattcct ggggcacgac ggacaggaag
       61 cggcgatagg tgcgcagatc gagcgtctgc atatcgcgac cgtcgagcag caggcgcccc
      121 gtcgtcggcc ggatgaagcc gatgaccagg ttcagcacgg tcgatttgcc cgcgcccgac
      181 ggccccacca gcgccaccgt ctccccggca gtcacactca gcgaaaaatc gcggatggcg
      241 ttggctgccg attcggggta ggcatagccc acgtcctcga aggtgatctc gccgattacc
      301 gattcgacgg ccgccttgcc ctcgttttgc tccagatcgg ggttctccag cacctcgccg
      361 atggatcgta tcgactccag ccccttgctg atctgcggcg tcatgttagc cagcaccatc
      421 acggcgttgg tcaacgtgga gaagtaggag gtgagcatga ccacctcgcc gatggtcagg
      481 gggatggcct gcgtccagta ggcccaggcg gcggccacca ggcacaggac gttgaacagg
      541 ttgaaggtca tccacgacag cgcgccgaac acggcgttga ccgaatccag ctgcgtgccg
      601 ctctcgcgaa cgcggtagag cgtctcgttg acccgctcca gcgcgtcttc ttccaggccg
      661 tgggcgcggg tgatagggat gagatgggtc atctcctcga cgcgcgtgga gagggtctcc
      721 agttcctggc gaaaggcgcg gttgcgttcg gccagcgtct ggcgcagccc gcgcaccagc
      781 agcgccgcca cggggacaac cagcacgtag aacaagagga actggggcac gcgccaggcg
      841 gtgatggcca gagcaaaaac gatattgctg acggccgaca gcccgctgtc gtagaagatg
      901 cgcgtcatct gctcgatgtt ctccacgtcg cgcagcacct tcgtttgcag cgacgccgca
      961 ctctgtcgag tgtagtaact gatggagaga tgttgcaggc ggcggcagat ggcggcgcgc
     1021 agacgggtct ccgtctcgcg cacggcgcgg ctcagatagc gagcatagag cgtgtgcagc
     1081 ggcacgttct ggaccagcag cagcaccagc aacagcgcgt tgaaccacaa accggacaag
     1141 tccgaccccg gcgtggagat gatgtcgatg atgttggccg tcagcgcggg caacatccac
     1201 accgggctgt gcttaatgat gaagaagacg gccgccacca gcatgcggcc gcgttcgggc
     1261 ttgtagaggg agagcagcgt gcgcagcggc cgctcgccct gatagcggcg ttccaggaga
     1321 acggaatcgg gcaagggggt ggtcatgctg gatttagaaa ccgagcttcc cggaagaagc
     1381 tcggtttcta tcagttaaaa cccttcaagc atcgacagat cggccacgcc acgggccaaa
     1441 ccggccacgc gctgcaggag cgccagccga ttctcgcgca cggccgcgtc gtcgtccatg
     1501 atcagcaccg cgtcgaacag ccgggtgata gccggttcta tccgacgcag cgaagcgacg
     1561 aacgtctcaa ctgtcccgtc cttggcagcc tcggcctcaa ccagcgcggc ggcgagcgct
     1621 tgctcttcgg gcaggcgcag atcgtccggc cggagcgtat agcgctcctt cagcggccgc
     1681 gtgatgcgta cgcagcgagc gtaggcgttg agcagcgttt cccagtcggg ggactggata
     1741 gcctcggcca gcgcggcggc ggcgcgggtg gccgcgtagg ggtcgtgggt ctgttcggcg
     1801 agcgtcgcct taacgacgct cgcggagtag ccggcgtcgc gcagcacgac atccagacgg
     1861 ccgccgatga aggccagcac ctcggcgcgc gtcttgtcgc tccaggccac gggcagcagc
     1921 gccccggccg catccagccc ggcgcgcaga tcaaacggct gccggttggc ggtcaggttc
     1981 tcgatcagat gcagcgcggc gcggcgcagg gcgaagggat cgttggaacc cttcggcccc
     2041 agtccggcgg cgaacagccc ggccagactg tcgagccgat cggccacggc cagcgccatg
     2101 cccgccgggc tgtcgctgac cgcttcatac tgtccggcga tggccgcggc cacctcgggc
     2161 gactcgccgc cgcgccgggc gtaatgaccg cccatcttgc cctgcaggct ggtcatctca
     2221 acgaccatgc tcgtcgccag atcggccttg gccagcgaag tggcgcgggc ggccgtggcg
     2281 cgggcagcct cgtccagccc cagcatcccg gccacgacgg ggacaagttg ctccagccga
     2341 tgggtcttgt ccaggaagga gccaagctcc gtctggaagg tcagcttgcc cagttcgggc
     2401 acgaaagcgg ccaacggttg gcgaatgtcc ttctcataga agaaggcggc gtcggcaaag
     2461 cgggcacgga tgacgtgctc gttaccgtcg cgcacgatat caaggtgctc ctcgtcgccg
     2521 ttgcgcacgg cgatgaaata gggcagcagc ttatcgccct tatagaccgg gaaatagcgc
     2581 tgatgcttgc gcatgaccgc caccaatacc atcggcggca gggccaggaa gcgctcctcg
     2641 aacgagccaa gcagcggcgt cggccgctcc accaggttgg tcacttcgtc cagcagcgcc
     2701 gggtcatcgg ggatgacgcc gccaaccgag cgggccagtt catgcgcccc ggcggcgatg
     2761 gccttgcggc gggcgtcggt atggagcacg atggtggcat tgtcgacgat ggttcgataa
     2821 tcggccgcat ggcgaatctc ccaggcgggc gagccgtagg gacgcaggcc aaacgtggtc
     2881 cgcccgctgc gcaccccggc gtattcaaac tccacgaccg ccgggccgtg gagcgccacc
     2941 agccagcgca gcggccggct gaagctaacg ttggaggcgt tccagcgcat cgacttctcg
     3001 aacttgatcc cggcgatgag gtccggcagg cgttcggcca gcacctcgag cgacggccgc
     3061 cccgtctcat gcaccacggc cgagacgtag cgcttgccgc cttcttcgac gacgctcagg
     3121 tcgtcgaccg acaggccgcg gctccgggcg aagccctcgg cggcgggagt aggcttgccg
     3181 ccggcatcga aggcgcggtc ggccggcggc cccttgacca cgctctccac atccgccccg
     3241 cgatcggcca ggccgtggac gatgaccgcc agccgtcgcg gtgtgccgtc aatctcgacg
     3301 cgctcgtagc tcagccgggc cgcgtcgagc agggcgggca cggccgcgcg cagctgcgcc
     3361 agcgccgagg tcaggtcgtc gtggggcaac tcctcgctgc cgatttcgag gacgaagggg
     3421 tcgccgtctt cgggaagccc acggaccacg gacgacagac caccgatatc attcgtcgtc
     3481 cgtggtctgt ggtctgtggt ccataccttg ttctccgcaa acggatactc cagccgctgc
     3541 cgctgctcca tataggccag cgagattttg tgggcgatgc ggcgcatgcg gccaaagtac
     3601 tgcgcccgtt cggtgacgcc gaccgcgccg cgcgtgtcca gcacgttgaa cagttgcgag
     3661 cacttgaggt tatagtcgtg ggccggggcc accagccccg cctccaggca gcgcaacgcc
     3721 tcgcgctcat aggtgtcgta gatcatcttc agcgcgtcca cgtcggccac gttgaaataa
     3781 tagttgcagt gttcgatctc ggcctgcagc aaggcgtcgc catacttcac acccgcgccg
     3841 tagtccatct cccacacgct gttcttgcct tgcaaggcca gcgcaatgcg gtcgaggccg
     3901 taggtgatct ccaccgacac cgggtccaga tcgatgccgc ccgcctgctg gaagtaggta
     3961 tactgggaga tctcctgccc atcgagccat acctcccagc ccagccccca tgcccccagg
     4021 gccggcgact gccagttgtc ctcgacaaag cggatgtcgt gctcgcgccg gctgatgccg
     4081 atggcttcca ggctgcccag atatagctcg atcgggttgc cggggtcggg cttcaggatg
     4141 acctgcaact gatagtgctg ctgcatgcgg ttggggttgt cgccgaagcg gccgtcgtcg
     4201 gggcgaatgc tcggctcgac ataggccaca ttccacggct cggggccgag gacgcgcaga
     4261 atcgtggccg ggttcatcgt ccccgcgccg acctgcacgt tgtaaggctg ccagacgagg
     4321 cagccctgcg cctgccagta atccagcagg cggaggatca tttgttggaa gttgagtttg
     4381 tcggaagaca ttatttagta accagtgaac agtaaacagt aaacagtcgc cgcgaatcaa
     4441 ccggcgatta tagcacagc
//
LOCUS       DAOMQG010000287         4446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8181_length_4446_cov_11.376907, whole genome shotgun sequence.
ACCESSION   DAOMQG010000287 DAOMQG010000000
VERSION     DAOMQG010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8181_length_4446_cov_11.376907"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..2476
                     /gene="lon"
                     /locus_tag="PLC06_13100"
     CDS             65..2476
                     /gene="lon"
                     /locus_tag="PLC06_13100"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRO91430.1"
                     /translation="MHEQYESKPIPVVPLRGGTVFPGVTTTVSIGRRASLAAVQAAIE
                     RGGDLLIAVQRDAEVETPATDDVFPVGILATARDMLRTPVGIQMVVELHRRVRLTGIE
                     GNDPFLIGRYEELAEEDAVDEDMKLEAIAYLDQYIQLVGEANQQVMTVTRASKTAGEL
                     ADYIAGLINLPFDVEVGLLTNLNGRSRLETILEHLRQEVRIADIRSKIQSDARDGAEK
                     AQRDYMLREQMKAIRKELGEDGEDAPEDLRTQIENAGMPPKVLERALSELKRLEYQGQ
                     QSAEASVIRTYLEWLIELPWTNETEDNFDITHVREVLDQDHYGLEDVKERIVEYVAVR
                     KLAGKDMKGAIINLNGPPGVGKTSIATSVARAIGREMVRISLGGVRDEAEIRGHRRTY
                     IGAIPGRIIRALRDAKTRNPVIVLDEIDKVGTDWRGDPASALLEVLDPEQNHSFTDHY
                     LEVPFDLSQVIFITTSNQLSTIPAALLDRMETITMPGYIEDEKLAIARQYLLKKQQDA
                     NGIGGVEVNIDDEALRGLIRYYTLEAGVRQLERNLGKVVRKLAVRVASGEAGPFAVTE
                     ADIEEIMGPRKFTYGVIEEQDEIGLVTGLAVNSYGGNTLPIEVSLSEGTGRITLTGSL
                     GDVMQESAQAALTYARANARSLGLEPALFDKVNVHIHVPDGATPKDGPSAGIAMATAI
                     ISAFTRRPVRRDVAMTGEVTLRGKVLPIGGLKSKTIAAHRAGIKTVLLPKDNAKDLPE
                     LPERVRQDLELIPVGHLDEVMAVALLEPVGPVMKVGEPEKTAEAAILPIPTNGIGGER
                     AGRIG"
     assembly_gap    2481..2580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2581..3531)
                     /locus_tag="PLC06_13105"
     CDS             complement(<2581..3531)
                     /locus_tag="PLC06_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO91431.1"
                     /translation="MSKEKLTASEWELVKNAPYWVNHALAEADGRVPFLVSRREGKAL
                     EKALKGYKTGNALIRDIIADEDDAPKEIGKASAAETDKAINRIAAIVEEKLGADDLAE
                     LRGFLLKIGREVAESAGEGSFGRGEKVSEKESAALARLESAMKVSTSFATAHMATQPK
                     PAAKPSTSAKPVPAAKPEPIIKPAAEDDEARKKAEQAKNREEARKRQEQAHAESEARK
                     KAEAEERQREAEAKAKADAEAAAAREEAARKEAEAKAAAAKYTQFIAEHTVQPGDNLS
                     FISQKYYGHQGNFRIIYEANKDVIGDNMNLIRPGQVLRIPK"
     gene            complement(3806..4267)
                     /locus_tag="PLC06_13110"
     CDS             complement(3806..4267)
                     /locus_tag="PLC06_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRO91432.1"
                     /translation="MKKEQLLIKIDQPWLAFRASYAGLTEAQMTQPGVTGDWSVMDII
                     GHVTVWEEEALKHLPHILEGKRPPRYSVLYGGIDAFNALKTEENRRRSPGEVLVRAED
                     VHRRLVAYIETAPEELFATETRFRRRIRLDTYSHYPIHTQAIHEWRAARGI"
     gene            complement(4369..4442)
                     /locus_tag="PLC06_13115"
     tRNA            complement(4369..4442)
                     /locus_tag="PLC06_13115"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4406..4408),aa:Gln,seq:ttg)
BASE COUNT          844 a         1362 c         1322 g          818 t
ORIGIN      
        1 agtgcccgat agaatctcag tgtcgccgtt tgtcgattcg ataagcggcg acaagggtaa
       61 agatatgcac gaacagtatg aatcaaaacc aatccccgtc gtgccgcttc gcggcggcac
      121 agtattcccc ggcgtcacga ccactgtgtc gatcggccgt cgggcatcgc tggcggccgt
      181 ccaggcagct atcgagcgcg gcggtgattt gcttatcgcc gtgcagcggg acgccgaagt
      241 tgaaacaccg gccaccgacg acgtgttccc cgtgggcatt ttggccaccg cgcgcgacat
      301 gttgcgtacg cccgtgggca tccagatggt cgtcgaactc caccgccgcg tgcgtctgac
      361 cggcattgaa ggcaacgacc cgttcctcat cggccgctat gaagagctgg ctgaggaaga
      421 cgccgttgat gaagatatga aactcgaggc tatcgcctat ctggatcaat atatccaact
      481 cgtcggcgag gccaatcagc aggtcatgac cgtgacccgt gccagcaaga cggccggcga
      541 actggccgac tacatcgccg gcctcatcaa cctgccgttc gacgtcgaag tcggtctgct
      601 gaccaatctc aacggccgct cgcgcctgga aaccatcctg gagcatctgc gccaggaagt
      661 gcgcattgcc gacatccgca gcaagattca gagcgacgcc cgcgatggcg cggagaaagc
      721 tcagcgcgac tacatgctgc gcgagcagat gaaagccatc cgcaaggagt tgggcgagga
      781 cggcgaagac gcccccgaag acctgcgcac gcagatcgaa aacgccggca tgccgcccaa
      841 ggtactggag cgcgctctca gcgagttgaa gcgcctggag tatcaggggc agcaatcggc
      901 cgaggccagc gtcatccgca cctatctgga gtggctcatc gagttgccgt ggacgaacga
      961 gaccgaggac aacttcgaca tcacccacgt tcgcgaggta ctcgatcagg atcactacgg
     1021 ccttgaggac gtgaaggaac gcatcgtcga atacgtcgcc gtgcgcaagc tggccggcaa
     1081 ggacatgaag ggtgcgatca tcaatctcaa cgggccgccc ggcgtcggca agacgtcgat
     1141 cgccacttcg gtggcgcggg ccatcggccg cgagatggta cgcatcagcc tgggcggcgt
     1201 gcgcgacgaa gccgagattc gcggccatcg tcgcacctac atcggggcca ttcccggccg
     1261 tatcatccgc gccctgcgtg acgccaagac ccgcaacccg gtcatcgttc tcgacgagat
     1321 cgacaaggtc ggcaccgatt ggcgcggcga cccggcttcg gccctgctgg aagtgctcga
     1381 cccggagcag aaccacagct tcaccgatca ctatctcgaa gtgccgttcg acctgagcca
     1441 ggtgatcttc atcaccacgt cgaaccagct cagcactatt ccggcggcgc tgctcgaccg
     1501 catggaaacg atcaccatgc ccggctacat cgaagacgaa aagctggcca tcgcccggca
     1561 gtatctgttg aagaagcagc aggatgccaa cggcatcggc ggcgtcgaag tgaatatcga
     1621 tgatgaggcg ctgcgcgggc tgatccgcta ctacacgctg gaagcgggcg tccgccaact
     1681 ggaacgcaac ctgggcaagg tcgtgcgcaa gctggccgtg cgcgtggcgt cgggcgaggc
     1741 gggaccgttc gcggtcaccg aggccgacat cgaagagatc atggggccgc gcaagttcac
     1801 ctacggcgtc atcgaggaac aggacgagat tggcctggtc accgggctgg ccgtgaacag
     1861 ctacggcggc aacacgctgc ccatcgaggt cagtctgagc gaaggcaccg gccgcatcac
     1921 cctgaccggc tcattgggcg acgtgatgca ggaatcggcc caggcagccc tgacttacgc
     1981 gcgggccaac gcccggtcgc tgggcctgga accggcgctg ttcgacaagg tcaacgtcca
     2041 tatccacgtg cccgacggcg cgacgcccaa ggatggcccc agcgcgggca tcgccatggc
     2101 gacagccatc atctccgcct tcacccggcg gccggtgcgg cgcgacgtgg ccatgaccgg
     2161 cgaggtgacg ctgcgcggca aggtgctgcc catcggcggc ctgaagtcga agaccatcgc
     2221 tgcccatcgg gcgggcatca agactgtgct gctgccgaag gacaacgcca aggacctgcc
     2281 cgaactgccg gagcgcgtgc gccaggacct ggagctaatc ccggtcggtc atctcgatga
     2341 ggtgatggcg gtggcgctgc tggagccggt tgggccggtg atgaaggtcg gcgagccgga
     2401 gaaaacggcc gaggcagcta tcctgccgat accgacgaac gggatcggtg gagagcgagc
     2461 tggtcgaatc ggctagatta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 cttcggaatc cgcagcacct gccccggccg gatcaggttc atgttgtccc cgatgacgtc
     2641 cttgttggcc tcatagatga tgcggaagtt gccctgatgg ccgtaatact tctgcgagat
     2701 gaagctcagg ttatcgcccg gctgcacggt gtgctcggcg ataaactggg tgtacttggc
     2761 cgcggcagcc ttcgcttcgg cttccttgcg ggcggcttcc tcgcgagcgg cggcagcttc
     2821 agcatcggcc ttcgccttgg cctcagcttc gcgctggcgt tcctcggctt cggccttctt
     2881 ccgggcttcg gattccgcat gggcctgctc ctgacgcttg cgagcctcct cccggttctt
     2941 ggcctgttcc gccttcttcc gggcttcgtc atcctcagcg gccggcttga ttattggctc
     3001 cggcttggcc gccggcacgg gcttggccga tgtgctcggc ttggcggcgg gcttgggctg
     3061 ggtcgccatg tgggccgtgg cgaacgaagt gctgaccttc atggcgctct ccaaccgggc
     3121 cagcgcggcc gattccttct cggaaacctt ttcgccccga ccaaagctgc cttccccggc
     3181 cgactcggcg acctcgcggc cgatcttcag caggaagccc cgcaactcag ccagatcatc
     3241 ggcacccagc ttctcctcga cgatggccgc gatgcgattg atcgccttgt cggtctcggc
     3301 cgccgacgcc ttgccgatct ctttcggcgc gtcgtcttca tcggcgatga tgtcacggat
     3361 cagggcattc ccggtcttgt aacccttgag tgccttttcc agcgccttgc cttcgcgacg
     3421 actgacgagg aacggcaccc ggccgtcagc ttccgccagc gcatggttga cccaatacgg
     3481 ggcattcttg acaagctccc attcggaagc ggtgagtttt tccttggaca ttgaatactc
     3541 ctatagttgt gataggcctg ttttgcaaca ggcagattga actgatcttt ttgacgattg
     3601 tgaaatgaca gcctgggaaa aatcggtgtc gcccggcgtg cggcagggtc agaataccac
     3661 cgtcaggccg gaatgaaacc ggccaaaaag ccggtatttt agccgttcat ggccggctac
     3721 ggtagctgcc gctactggaa gcagatatga gcgacacgct cgctcacgct cttgtaactg
     3781 tatttttaat cggcgaatcc ttaatctaga ttcctctcgc cgctcgccac tcatgaatgg
     3841 cttgcgtgtg aatcggataa tggctgtagg tgtcgaggcg aatgcggcga cgaaagcgcg
     3901 tctcggtggc aaacaattcc tcaggcgcag tctcgatata ggcgacaagc cgccggtgca
     3961 catcttcggc tcgcaccaac acttcgcccg gcgagcgccg tcggttctcc tccgtcttca
     4021 gcgcgttgaa ggcgtcgatg ccgccataga gaaccgagta acgtggcggc cgcttccctt
     4081 ccaaaatgtg gggcagatgc ttcagcgcct cctcctccca cactgtcaca tggccgatga
     4141 tatccatcac cgaccagtcg ccggtgacgc cgggctgggt catttgcgcc tcggtcagcc
     4201 cggcatacga tgccctgaaa gccaaccatg gttgatcaat cttgatcagc aactgctctt
     4261 ttttcacgca acgttcctaa acacgacaag tagtcgttat aagaagaaca aagggggcag
     4321 tcattgtgca cccccttttt gaaactcgta ataaattggt ctcaacagct ggggcggctg
     4381 gattcgaacc aacgaatgcc ggattcaaag tccggtgcct taaaccactt ggcgacgccc
     4441 caagac
//
LOCUS       DAOMQG010000288         4435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8206_length_4435_cov_9.911187, whole genome shotgun sequence.
ACCESSION   DAOMQG010000288 DAOMQG010000000
VERSION     DAOMQG010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8206_length_4435_cov_9.911187"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLC06_13120"
     CDS             <1..888
                     /locus_tag="PLC06_13120"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HRO91433.1"
                     /translation="TVGEALAEAEIALGALDNVEPPREAVLVPEMAILIERAAPYVVV
                     ADGHTVSVQSDSTQVFDILADNGIALGPLDYTRPATGAAVAPGDTIEVVRVTERIDTV
                     EEAVPFETVFEAAPDLEIDTLIEAAPGSNGVLQRLTRVRLENGVEVSSEPGGESLLVA
                     PVNRVLRYGTNIIMRTIDTPEGPVEYWRKVTMRVTSYTATSAGKKPGEPGYGITASGV
                     QAGTGVIAVDPKVIPFRSYVYVPGYGVAFAGDTGGGIKGRFVDLGYDEDEFQSWRGTV
                     DVYYLGPPPPAEEIRWILP"
     gene            1158..2168
                     /locus_tag="PLC06_13125"
     CDS             1158..2168
                     /locus_tag="PLC06_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91434.1"
                     /translation="MTSIIERIKNRQSRPKVVLILALLGIFLVMLGSAAAQSGDEVVP
                     TFTIENVVVDGSVSILAQDFPANQDFVVTMGPIGTLGINGTPVAITNSGVFGAFSATY
                     PIPPNLVGARQIAIRLQSPQGYYSYNWFWNNLTEPAPLPIPTISIDSVAVNEFVTIRT
                     HNFPPDTTFLITMGHMGTLGIDGTPVGTLYSGLGGEMTATFPIPVLLKGLERIAIRAQ
                     SNPFYAYNWFNNQTEPLPTPTFAICGVLRDDMVVIRTDPSFPPNRDFTVMMNYMGTLG
                     IDGYVVGGFNSGPTGVMTATYLIPSGLRGLDQIHIRAEEVGGPFYSYNYFDNQTAVYC
                     AP"
     gene            2431..>4435
                     /locus_tag="PLC06_13130"
     CDS             2431..>4435
                     /locus_tag="PLC06_13130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO91435.1"
                     /translation="MTAPGTQQETGFLPGNPVSKIARLLILLLLILLGYWLRVHNLDA
                     FSFWTDEGLTPERSGYAIAQILRNDILIQGVVTTDTHPPLYYLILHLTRQLFGLSDFA
                     FRYPSVLFGVLLIPLLYQLGRRMGGRAVGLIAALLAAVNPLQVYYSQEARMYSLLLLL
                     VTGMSYVLWRAIQDTRYEIRGDSRRPTSSEARSSLVSRISYLVFPYLLLAALAVYTHY
                     TAVFIVAAQALLWAWVLWRAIRDTRYEIRGDSRRPTSSEARSRFVSRTSYFVVACLIL
                     AVLVAIPLIPYTIPRLLAGAEANYYRVAPLTMLLDVVRFFNLGLTVDHARPLAGALNV
                     LALGLALLGVRQITNYELRITNEERAPHSYLVSRISYLIPPCFLLSWLLAVVFGLMLG
                     SLIFKPMYQGVRHIMAGSPAYLLLIAFGLAALTRYEIRDTKYESYPLHSYLVSRISYL
                     LLGLLLVGYGVALANLYTDPAYVKDDFRAIVRFIETRAGDRDVIVYNNAVLLPLHDHY
                     RRRDDLAVTALPAYPQFATGAEPELAALARDYDRVWFITDPPADKRDDDKLIQAWLDA
                     NLTETINRLFPARTTEARVIAYRTGEGEKGGEGEGETGSADSPLLPFSLSPSLSLIGF
                     TYDSPLELPTLWVDLLWRGERPVVNTSLLFTLTGPDGKEYFREA"
BASE COUNT          763 a         1528 c         1281 g          863 t
ORIGIN      
        1 accgtcggcg aggcgctggc cgaggcagag atcgccctgg gcgcgctcga caacgtcgaa
       61 ccgccacgcg aggcggtgct cgtcccggag atggccatcc tcatcgagcg cgccgcgccc
      121 tacgtcgtcg tcgccgacgg ccacaccgtg agcgttcaaa gcgactcaac gcaggtgttt
      181 gatattctgg ccgacaacgg catcgccctg gggccgctgg actacacgcg cccggcgacc
      241 ggcgcggccg tggcccccgg cgacacgatt gaggtcgtgc gcgtcaccga gcgcatcgac
      301 accgtggagg aagctgtgcc gttcgagacg gtgttcgagg cggcccccga tctggagatc
      361 gacacgctga tcgaggctgc gcccggcagt aacggcgtgc tccagcgcct cacgcgcgtg
      421 cggctggaga acggcgtcga ggtatcgagc gaacccggcg gcgagagcct gctcgtcgcg
      481 ccggtgaatc gcgtgttgcg ctacggcacg aacatcatca tgcgcaccat tgacacgccg
      541 gaagggccgg tggaatactg gcgcaaggtg accatgcgcg tcacgtccta taccgccacc
      601 agcgcgggca agaagccggg cgaaccgggc tacggcatca ccgccagcgg cgtccaggcg
      661 ggcacgggcg tcatcgccgt cgacccgaag gtcatcccct ttcgcagcta cgtctacgtg
      721 ccgggctacg gcgtggcctt cgccggcgat acgggcgggg gcatcaaggg ccgcttcgtc
      781 gacctgggct acgatgagga tgaattccag tcgtggcgcg gcacggtgga cgtctactac
      841 ctcggcccgc cgcccccggc cgaagagatc cgctggatat tgccgtagac gctggtactc
      901 tggtactctt ttctctcata atcgccaaaa ccccgctatt tttcacgccg gttttatgtg
      961 aaccatcccc agcttgtggc atgataaaac gcgagggtcg tccgcgctga ttgccggcga
     1021 cccttgcctg tttgccggcg gcgcgggacg acttttgggt cgcgctttcc tcaatccggc
     1081 ggcgctgctc ctcataatag caaccctgtc cggaggctac cggaaacaca aatccatcat
     1141 tggagggatt acacaccatg acatccatca tcgagcgcat caaaaaccgg caatcgcggc
     1201 cgaaagtcgt cctcattctg gccctgctgg ggatattcct ggtcatgctg ggcagcgcgg
     1261 ccgcccagag cggcgacgaa gttgtgccga cgttcaccat cgagaacgtg gtcgtcgacg
     1321 gcagcgtgtc catcctggcc caggacttcc cggccaacca ggatttcgtc gtcacgatgg
     1381 ggccaatcgg cacgctgggc atcaacggca cgccggtggc catcaccaat tcgggcgtgt
     1441 tcggggcgtt ctcggccacc taccccatcc cgcccaacct cgtgggcgca cgccagatcg
     1501 ccatccggct gcaaagcccg cagggctact acagctataa ctggttctgg aacaacctga
     1561 cggaaccggc cccgctcccc atcccgacga tctccatcga ctcggtggcg gtgaacgaat
     1621 ttgtgaccat tcgcacccac aacttcccgc ccgatacgac cttcctgatc acgatgggcc
     1681 acatgggcac gctgggcatc gacggcacgc cggtgggcac gctctattcg gggctgggcg
     1741 gcgagatgac ggccaccttc cccatcccgg tgctgctcaa ggggctggag cgcatcgcca
     1801 tccgcgccca gagcaacccg ttctacgcct ataactggtt caacaaccag accgaaccgc
     1861 tgccgacgcc gaccttcgcc atttgcggcg tgctccgcga cgacatggtg gtcatccgca
     1921 ccgaccccag cttcccgccc aaccgcgact tcaccgtgat gatgaactac atgggcacgc
     1981 tgggcatcga cggctacgtc gtgggcggct tcaactcggg gccgacgggc gtgatgacgg
     2041 cgacctatct catcccgtcc ggcttgcgcg ggctggatca gattcatatc cgcgccgaag
     2101 aagtgggcgg gccgttctac agctacaact acttcgacaa tcagacggcc gtctactgcg
     2161 cgccgtaacc gattcgaaca cagcctctca ataaggcaga cgtggcagca gggcgggcac
     2221 gcaggcctgc cctctgccac ggataactcc cagggaaacg gtctatcggg tcgtcgggtg
     2281 cgcatcatgg cccggcggcc cgatagccgt tattcagact gagatagaaa ccgggtttct
     2341 cggaagaaac ccggtttcta aaatcctcag agcctagcct tcccttaata tcttcgcctc
     2401 tcgccccgcc cgcgctacaa tttcaggtat atgaccgcac ccggcactca gcaagaaacc
     2461 gggtttcttc cgggaaaccc ggtttctaaa atcgctcggc tcctcatcct gctgctcctc
     2521 atcctgttgg gctactggct gcgcgtccac aacctcgacg ccttcagctt ctggaccgac
     2581 gagggcctca cgccggagcg ctccggctat gccatcgccc aaatcctgcg caacgacatt
     2641 ctcatccagg gggtcgtgac caccgacacc cacccgccgc tctactacct gatcctgcac
     2701 ctgacccggc agctgttcgg cctgtcggat ttcgccttcc gctatccgtc cgttctgttc
     2761 ggcgtgctgc tcatccccct gctctatcag ctggggcggc gcatgggcgg ccgcgcggtg
     2821 ggtctcatcg ccgctctgct ggcggccgtc aacccgctgc aggtctacta tagccaggaa
     2881 gcgcgcatgt attcgctgct gctgctgctg gtgacgggga tgagctatgt gttgtggcga
     2941 gcgattcagg atacgagata cgagatacga ggcgactctc gtcgccctac gagttcggaa
     3001 gcgcgctctt cactcgtatc tcgtatttcg tatctcgtat ttccctatct cctcctcgcc
     3061 gcgctcgccg tctacaccca ctatacggcc gtcttcatcg tggcggcgca ggcgttgttg
     3121 tgggcgtggg tcttgtggcg agccattcga gatacgagat acgaaatacg aggcgactct
     3181 cgtcgcccta cgagttcgga agcgcgctcc cggttcgtat ctcgtacctc gtatttcgta
     3241 gttgcctgtc ttatcctcgc cgtcctcgtg gccatccccc tgatccccta caccatcccg
     3301 cggctgctgg ccggggccga agccaactac taccgcgtgg ccccgctcac catgctgctc
     3361 gacgtggttc gcttcttcaa cctcggcctc accgtcgacc acgcccgccc gctcgccggc
     3421 gctctcaacg tgctggccct ggggctggcg ctgcttggcg tccgccaaat tacgaattac
     3481 gaattacgaa ttacgaatga ggagcgcgct ccccactcgt atctcgtatc tcgtatctcg
     3541 tatctcatcc ccccctgctt cctgttgtcc tggctcctcg ccgtcgtctt cggcctcatg
     3601 ctcggctcgc tgatcttcaa gccgatgtat cagggcgtgc ggcatatcat ggccggcagc
     3661 ccggcgtatc tgctgctgat cgccttcggc ctcgcggcgc tgaccagata cgagatacga
     3721 gatacgaaat acgagtcgta ccctcttcac tcgtatctcg tatctcgtat ctcgtatctc
     3781 ctcctcggtt tgctgctcgt cggctacggg gtcgctctgg ccaatctcta caccgacccg
     3841 gcctacgtga aggacgactt ccgggccatc gtgcgtttca tcgagacgcg ggcgggcgat
     3901 cgcgacgtaa tcgtctacaa taacgccgtg ttattgcccc tgcatgatca ttaccggcgg
     3961 cgcgacgacc tcgccgtcac cgcgctgccc gcctatcccc aattcgccac cggcgcggag
     4021 ccggaactgg cggcgctggc ccgcgactac gaccgcgtct ggttcatcac cgaccccccg
     4081 gccgacaagc gcgacgacga caaactcatc caggcctggc tcgacgccaa cctgaccgaa
     4141 acgatcaacc gcctcttccc cgcccgcacc accgaggcgc gggtgattgc ctaccggacg
     4201 ggggaagggg agaaaggggg agagggagaa ggggagacag ggagcgcaga ctctcccctt
     4261 ctccccttct ccctttctcc ctctctctct ttgatcggct tcacctacga ctcgccgctg
     4321 gaactgccca cgctgtgggt cgatctgctc tggcgcgggg agcggccggt ggtcaatact
     4381 tcgctgctgt tcacgctgac cgggccggac ggcaaggagt atttccgcga ggcgc
//
LOCUS       DAOMQG010000289         4426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8216_length_4426_cov_8.734615, whole genome shotgun sequence.
ACCESSION   DAOMQG010000289 DAOMQG010000000
VERSION     DAOMQG010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_4426_cov_8.734615"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1099
                     /locus_tag="PLC06_13135"
     CDS             320..1099
                     /locus_tag="PLC06_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91436.1"
                     /translation="MDDSILLETRELVRVFGGLRAVDGVSMRVKAHTLHTIIGPNGAG
                     KTTFFNLLSGNIRPTSGKVIYKGRDITEMPAYRRVHIGIGRSFQITNVFPNLTVLENV
                     RLAAQAVGGDNYKLWRHFRHFRAYEERAWAILQQVGLADRAALPARLLPHGGKRKLEL
                     GILLAADPDLLLLDEPTAGMAREEIPMLMEVIQSVREVGNKTIILVEHKMDLVMNVSD
                     RISVMHQGQLLAEGTPLEIAGNPTVQKAYLGDLYGDLTGGK"
     gene            1110..1826
                     /locus_tag="PLC06_13140"
     CDS             1110..1826
                     /locus_tag="PLC06_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91437.1"
                     /translation="MTTTQKNVLEVRDIQTFIGQFHILDGVSVDVPDGSITVLLGRNG
                     AGKTTTLKSILGLTPPRAGAVIFKGKEIQGQPAYRTAAKGIGYVPEHRAIFRDLSVEE
                     NLRIAERKAGDLARREEFIFGLFPDLKRLIKLPGGNLSGGQQQMLAVARALVPDNELL
                     LIDEPSEGLAPVVIEQMMEAIRRLSEHTTVLLVEQNFIMASRLADRYTIIETGQSVKS
                     GMMADLVDDEATIQRYLGAA"
     gene            1847..3355
                     /locus_tag="PLC06_13145"
     CDS             1847..3355
                     /locus_tag="PLC06_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO91438.1"
                     /translation="MASIWKNNRAVLIVAALAVVLFGGMIQSMEPKVWVSVMLSGLTL
                     ASLYFLVASGLSLIFGLMDVLNFAHGVLFVLGAYVGLATYMNPRLLLNTLPIFFVLIA
                     AVMMAQHVGRAFWPRISGKRNQRLATIGLLLAAALLMILALRGFPIRALNAFNLTAVG
                     GAVTTANAQEPLAMMFRRLLLLALAGVPIGILLSPTDRQDEVPLRPLWQTALITGGAA
                     LLGVVLLLVRDSGETWMLALSPDIRFLLALIMGTIAGALGGVAIETALIRPFYGNSVT
                     QLVLTLGLSIAGIDLIEAMFGEEANPPMPPPTLFGENCRSDSLINWFSENCQSIDVLG
                     RAFPTYRIFIIVVGVLVFVAVGLMLRRTRLGMIIRAGVQDSEMVQALGINVRRIFTLV
                     FALGSGLAAMGGVIAAPYLGVFPGMGAIYQLQAFIAVVIGGMGSFIGAAVGTIILGMA
                     RAFGDHFVAAGISLPFLDTIIKGSPAIARASTVLIMALVLLIKPSGIFGKKE"
     gene            3362..>4426
                     /locus_tag="PLC06_13150"
     CDS             3362..>4426
                     /locus_tag="PLC06_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO91439.1"
                     /translation="MNDLSINQSSSIRRLLSDWWPYLLVLAFLVLFPLLAGATIGSPV
                     EGASGLSGLMQRAESGAAKFWQGMIIQILIFAIFAMSYDLLLGYTGILSFGHAMFFGT
                     GGYAIAILIGRNFNWSFTMALAAVVIVALIQSIIFGVLSLRVKGVYLAMVTLAFAEMF
                     FILAEAGDFRTFTGADDGLHGFPVPGYLSATDHRTRFYYLTLVFFVAAFLIIKRLVDS
                     PTGKVMVATRENENRATMIGFNTFWYKLTAFVAAGVFAALAGALSASYKVSVTPSMLS
                     LGTTIDALAMTIIGGLGTLVGPVLGAVIIQLLGYWLERWFGASWTLIYGLIFMLIVIF
                     LPFGIVGTLRARSFQLREGWR"
BASE COUNT          717 a         1357 c         1414 g          938 t
ORIGIN      
        1 ggcacggtca tcatccgtcc gtccgaccat gtggccctgc agaatctgta cttcgtgcgc
       61 gtgaccaacg tgacggatcc cgatttcaag ttcgtcgagt tggtgaagga attccaaccc
      121 gaagagaccg caccgccgtg cgccctggtg gacgcctacg cggatcgctg caactaaacg
      181 tttttgtgat gtgagcaaga gggagacggg ttcgtctccc tcttgttgta atggcgtttg
      241 tttgtagtcg acgcttcagc gccatcggct aattgataat agcagcccct tgcccatgcg
      301 agcggccgag gatctgtgta tggatgattc aatccttctg gaaacccgcg aactggtgag
      361 ggtctttggc ggcctgcggg cggtcgatgg ggtcagcatg cgggtgaagg cccacacgct
      421 ccacaccatc atcggcccca acggggcagg caagaccacc ttcttcaatc tcctgagcgg
      481 caacattcga ccgaccagtg gcaaggtgat ctacaaaggt cgggacatca ccgagatgcc
      541 cgcctatcgt cgcgtccaca ttggtatcgg ccgctccttc cagatcacca acgtcttccc
      601 caatctgact gtgctggaga atgtgcgcct ggcggcccag gccgtgggcg gcgacaacta
      661 caagctatgg cgtcactttc gccatttccg ggcctatgag gagcgcgcct gggccatttt
      721 gcagcaggtg gggctggccg accgggccgc gctgcccgcc cgcttgctgc cccatggcgg
      781 caaacgcaag ctggagctgg gcatcctgtt ggccgccgac cccgatttgc tcctgctcga
      841 tgagccgacg gccggcatgg cccgcgagga aatcccgatg ctcatggaag tcatccagag
      901 tgtgcgcgag gtgggcaaca agaccatcat cctcgtggag cacaagatgg acctggtgat
      961 gaatgtgtcc gaccgcatct cggtgatgca tcaggggcag ctgttggccg aggggacgcc
     1021 gctggagatc gccggcaacc cgacggttca gaaggcttat ctgggcgacc tctacggcga
     1081 cctgaccggg gggaaatagg gcgagcgcca tgaccacgac gcaaaaaaac gtcctggaag
     1141 ttcgggacat ccagaccttc attggtcaat ttcatattct cgacggcgtg tcggtcgatg
     1201 tgcccgacgg cagcatcacc gttttgctgg ggcgcaacgg cgcgggcaag acgacgacgc
     1261 tcaagtcgat cctggggctg acgccgccgc gcgccggggc cgtcatcttc aagggcaagg
     1321 agattcaggg gcagcccgcc tatcgcacgg cggccaaggg catcggctac gtgccggagc
     1381 atcgcgccat ttttcgcgat ctgtccgtgg aggagaatct gcgcattgcc gaacgcaagg
     1441 cgggggattt ggcccggcgc gaggagttca tcttcggcct atttcccgat ctcaagcggt
     1501 tgatcaagct gcccggcggc aatctgtcgg gtggccagca gcagatgctg gccgtggccc
     1561 gcgctctggt gcccgataac gagctgctgc tcatcgacga accaagcgaa gggctggccc
     1621 cggtggtcat cgagcagatg atggaagcca tccgtcgcct gtccgaacac acgacggtgc
     1681 tgctggtgga gcagaacttc atcatggcca gccgcttggc cgaccgctac accattattg
     1741 agaccggcca atcggtgaag agcggcatga tggccgatct ggtcgacgac gaagcgacga
     1801 tccagcgcta tctcggcgcg gcatagaaag agcaagcgag ttgcatatgg catcaatctg
     1861 gaaaaataac cgcgccgtgc tgatcgtggc cgcgctggcg gtcgtcctct ttggcggcat
     1921 gattcagtcc atggagccga aggtctgggt gtcggtcatg ctctccggcc tgacgctggc
     1981 ttcgctctat tttctggtgg cgtcggggct gtcgctcatc ttcggcctga tggacgtgct
     2041 caatttcgcc catggcgtgc tgttcgtgct gggggcctat gtggggttgg cgacctacat
     2101 gaacccgcgg ctgttgctca acacgctgcc gatcttcttt gtgctcatcg ccgcagtcat
     2161 gatggcgcaa catgtgggcc gggccttctg gccgcgcatc agcggcaagc gcaaccaacg
     2221 actagcgacc atcggcctgc tgctggcggc cgcgctgctc atgatccttg ccttgcgcgg
     2281 ctttcccatt cgcgcgctca acgcgtttaa cctgacggcc gtcggcgggg cggtgacgac
     2341 ggccaacgcg caagagccat tggcgatgat gttccgccgc ctgctgctgc tggcgctggc
     2401 cggcgtgcct atcggcatcc tcctgtcgcc caccgaccgc caggatgagg tgccgctgcg
     2461 gccgctgtgg caaacggcgc tcatcaccgg aggggccgcc cttcttgggg tcgttctgct
     2521 gctggtgcgc gactccggcg agacgtggat gctggccctg tcgccggaca ttcgctttct
     2581 gctggccctc atcatgggca cgattgccgg cgcgttgggc ggcgtggcca tcgagacggc
     2641 gctcattcgg ccgttctatg gcaactcggt cactcagctg gtgctgacgc tgggcctgag
     2701 catcgccggc atcgacctga tcgaggcgat gttcggcgag gaagccaacc cgcccatgcc
     2761 gccgccgacg ctcttcggcg agaactgccg ttccgattcg ctcatcaact ggtttagcga
     2821 gaattgtcag tcgatcgacg tgctggggcg ggccttcccc acctatcgga tattcatcat
     2881 cgtggtcggc gtgctggtct ttgtggcggt ggggctgatg ctgcgccgga cgcggctggg
     2941 catgatcatc cgcgccggcg tgcaggacag tgaaatggta caggcactcg gcatcaatgt
     3001 ccgccgcatc ttcacgctgg tctttgcgct gggttccggc ctggcggcca tgggcggggt
     3061 catcgccgca ccctatctgg gcgtcttccc cggcatgggc gccatctacc agttgcaggc
     3121 gttcatcgcc gtggtgattg gggggatggg cagcttcatc ggcgcggccg tgggcacgat
     3181 tatcctgggc atggcccgcg cctttggcga ccattttgtg gccgcgggca tcagcttgcc
     3241 gttcctcgac actatcatca aaggcagccc ggccatcgcc cgcgcgtcca ccgtgctgat
     3301 catggccctc gtgctgctga tcaagccgtc ggggattttt gggaagaaag aatagtctac
     3361 tatgaacgat ctatctatca accagtcttc ttccatccgc cgcctgcttt ccgattggtg
     3421 gccttatctg ctcgtgttgg ccttcctggt gctgtttccg ctgttggccg gagccacgat
     3481 tggttctccg gttgaggggg cgagcggcct cagtggcctg atgcagcggg ccgagagcgg
     3541 cgcggccaag ttctggcagg gcatgatcat ccagatcctc atcttcgcca tcttcgccat
     3601 gagctatgac ctgctgctgg gctataccgg catcctgtcg ttcggccacg ccatgttctt
     3661 cggcaccggc ggctatgcca tcgccatcct catcggccgc aacttcaact ggtcgttcac
     3721 catggcgctg gcggcggtcg tcatcgtcgc actgattcag agcatcatct tcggcgtgtt
     3781 gtcgctgcgg gtgaaggggg tctatctggc catggtcacg ctggccttcg ccgagatgtt
     3841 cttcatcctg gccgaggcgg gcgactttcg caccttcacc ggcgcggatg acggcctcca
     3901 cggcttcccg gttccgggct atctgagcgc caccgaccac cgcacgcgct tctactattt
     3961 gacgctggtg ttcttcgtgg ccgcctttct gatcatcaaa cggctggttg attcgccgac
     4021 gggcaaggtg atggtggcca cccgcgagaa cgagaaccgg gccacgatga ttggcttcaa
     4081 caccttctgg tacaagctga cggcatttgt ggccgcgggc gtttttgccg cgctggccgg
     4141 agcgttgtcg gccagctaca aggtcagcgt cacgccgtcg atgttgtcgc taggcaccac
     4201 gatcgacgcg ctggcgatga ccatcatcgg cgggctgggc acgctcgtgg ggccggtgct
     4261 gggggcggtg atcatccagt tgctcggcta ttggctggag cgctggttcg gcgcgtcgtg
     4321 gacgctcatc tatggcctga tctttatgct catcgtcatc ttcctgccct tcggcatcgt
     4381 cggcacgctg cgggcgcggt cgttccagct cagggaagga tggcga
//
LOCUS       DAOMQG010000290         4420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8230_length_4420_cov_10.270561, whole genome shotgun sequence.
ACCESSION   DAOMQG010000290 DAOMQG010000000
VERSION     DAOMQG010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8230_length_4420_cov_10.270561"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLC06_13155"
     CDS             complement(<1..443)
                     /locus_tag="PLC06_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRO91440.1"
                     /translation="MTEPPVTPIIPESAWIAETATVRGDVTLGEDVSIWFAAVLRGDE
                     APITVGDRTNIQDGAVLHVSAGFPCVVGREVTIGHGAVVHGCTVEDGALIGIRAVVLD
                     GAVIGAGAVVGAGAVVPPGMHLPPGMLALGVPAKVVAPLTDAQRSA"
     gene            complement(447..1346)
                     /locus_tag="PLC06_13160"
     CDS             complement(447..1346)
                     /locus_tag="PLC06_13160"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRO91441.1"
                     /translation="MDFLTEATFWRLLAAAGIFLVGLIYIRGWRRLQAFHKGEPPRDA
                     PASMRSLRFFMAGLGLLAVAFISPLGYLSTQYFSARIVQHMLLVASIPSLLMFADPVP
                     ALLHGLPEQWRAALSAAGKSPQQYSAGRRFVRWATAPNITLLTFLSVCWLWYDPTIHA
                     ATLRYSVVHAVELLSLLSIALLNWWHITNAWPHTHGKMPPFVRVLYTFISIWPIKIIG
                     LILLFLQHPLYQYPASFQFRGLQINDYIFGGIIAWIVSGLAYVIASVALVNEWLSSEI
                     DKPTLPESQWATDEAMLAPGIKR"
     gene            complement(1423..3252)
                     /locus_tag="PLC06_13165"
     CDS             complement(1423..3252)
                     /locus_tag="PLC06_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRO91442.1"
                     /translation="MIEQPNLSLQEDMGIKPSKSFRMIYVSILAAVIGAISGLLAFAL
                     FSLIGLLWNLVFFQRVATELTDLETNPIGLWMLIVPAIGGLIVALMAKYGTEKIKGHG
                     IPEAMEAVLENRSRVSPRVAIFKPIATAVAIGTGGPFGAEGPIIQTGGAMGSLLGQIL
                     PTTAVERKVLLACGAAAGMAATFNTPIAAVIIAIELLLFEFKSRSFIPLVIATVVATT
                     VRIPFLGRGPLFQVSITDFGIPRALPFYILLGLLCGLVAVGFTKLLYLTEDLFDRIPV
                     NKFWLPVIGGFGLGIIGYFVPRVLGVGYGTISGILNNQLTLSVLLSVMIFKTLALLVS
                     LGSGTSGGLLAPLFMASAAMGGAMAILLNSAMPSLQLEPGAFALAAMAAVFGAASRST
                     FAFIIFGFEITQDYNAILPLMLVCVIADAVGLILMRNTVMTEKLARRGLRVPAEYDAD
                     PLQQVTVGDMMVTTTLNLRSDMRVKELADHIARGEPDYVRHQAFLIVDEDDNLAGIIT
                     RGDLLVALEEPAGGDLSVLHAGTRQVVVAYPDEMIHDALNKLVHNDIGRLPVVSRENP
                     RRVVGYLSRGAILRARRRRADEEHRREEGWLERLKNGGVGSRA"
     gene            3401..3847
                     /locus_tag="PLC06_13170"
     CDS             3401..3847
                     /locus_tag="PLC06_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRO91443.1"
                     /translation="MAQPISRTEYEKLASFRYALRQFLRFSETAAREAGITPRQHQAL
                     LAIAGHPGREEVMMSELAERLQLRHHTTVELIDRMAAQELVVREPGVEDRRYVYVSLT
                     PQGHDVLARLSVAHREELKRIGPQLNLLLHELSTVGEADTPTNASR"
     gene            4032..>4420
                     /locus_tag="PLC06_13175"
     CDS             4032..>4420
                     /locus_tag="PLC06_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRO91444.1"
                     /translation="MPKPITSPLTDPVYTRAPYWKDVPDEKWMDWRWQMSHRLNSVEE
                     LGRVINLTDSERRALTAQGLFRVDITPYFASLIDPDDPHCPVRKQVIPTDREMVPFES
                     MMEDSLAEDKHSPVPGLVHRYPDRVLML"
BASE COUNT          880 a         1364 c         1403 g          773 t
ORIGIN      
        1 gcggagcgct gggcgtcggt cagtggggcg acgaccttgg ccggcacgcc gagggccagc
       61 atccccggtg gcaggtgcat ccccggcggc acgaccgccc ccgcgcccac caccgccccc
      121 gcgccgatga ccgcgccgtc gagcacgacc gcccggatgc cgatgagcgc gccgtcttcc
      181 accgtgcagc cgtggacgac cgccccgtgg ccgatggtga cctcgcggcc gacgacgcac
      241 ggaaacccgg ccgagacgtg gagcacggcc ccgtcctgga tgttcgtccg gtcgccgacg
      301 gtgatcggcg cttcgtcgcc gcgcagcacg gccgcgaacc agatgctgac gtcttcgccc
      361 agcgtgacgt cgccgcgcac cgtcgccgtt tcggcgatcc acgcgctttc gggaataatg
      421 ggggtgacgg gtggctcagt catcgtttaa cgcttgatcc ccggcgcaag catcgcctcg
      481 tcggtggccc attgcgattc gggtagcgtt ggtttgtcga tttcgctcga caaccattcg
      541 ttcaccagag cgactgacgc gatcacatag gccaggccgc tgacgatcca ggcgatgatc
      601 ccgccgaaga tgtagtcgtt gatctgcaac cccctgaact gaaaggatgc cggatactga
      661 tagagaggat gctgaaggaa taatagtata aggccgatga tcttgatggg ccagatgctg
      721 atgaaggtat agagcacgcg cacaaatggc ggcatcttgc cgtgggtgtg cggccaggcg
      781 ttggtgatat gccaccagtt gagcagggct atgctcagca gactgagcaa ttcgacggca
      841 tggacgacgc tataacgcag cgtcgccgcg tgaatggtcg ggtcatacca gagccagcag
      901 acgctgagga aggtcagcaa ggtgatgttc ggtgcggtcg cccaacggac gaagcgacgg
      961 ccggcgctgt attgttgcgg cgatttcccg gcggccgaca gggctgctcg ccattgttcc
     1021 ggcaggccat ggagcagcgc cggaacgggg tcggcgaaca tcagcaagct ggggatggac
     1081 gctaccagca gcatgtgctg gacgatgcgg gcgctgaaat actgggtcga cagataaccg
     1141 aggggcgaga tgaaggcgac cgccagcagc cccagcccgg ccatgaagaa ccggagcgag
     1201 cgcatcgagg ctggggcgtc cctgggcggt tctcctttat ggaacgcctg tagtctccgc
     1261 cagccgcgaa tatagatcag ccccacgagg aaaatgcccg cggctgccaa caatcgccag
     1321 aatgtcgctt cggtcagaaa gtccatcgcg ttggggattg tagcaagccg cgggcatggg
     1381 gcgcaacggg tttagccgtc gtttatcgat cggattgccg gtttaggccc gggaaccaac
     1441 gccgccgttc ttcaatcgtt ccagccaacc ctcttcgcgc cggtgttcct cgtcggcccg
     1501 acgacgtctg gcgcgcagga tggccccacg actcagatag ccgacgaccc ggcgcgggtt
     1561 ctcgcgactc accaccggca ggcggccgat gtcgttatgc accagcttgt tgagcgcgtc
     1621 gtggatcatc tcgtccgggt aggcgacgac gacctggcgg gttcccgcgt gcaggacgga
     1681 gagatcgccg ccggccggtt cttcgagcgc caccagcaga tcgccgcggg tgatgatgcc
     1741 cgccagattg tcgtcttcat cgacgatgag aaaggcctga tggcgcacgt agtcgggttc
     1801 gccgcgcgcg atgtggtcgg ccagctcttt cacgcgcatg tcgctgcgca ggttgagcgt
     1861 cgtcgtgacc atcatgtcgc cgacggtgac ctgctgcaag gggtcggcat cgtattcggc
     1921 ggggacgcgc aacccgcgcc gcgccagctt ctccgtcatc accgtgttgc gcatgaggat
     1981 tagccccacg gcgtcggcga tgacgcagac cagcatcagg ggcaggatag cgttgtagtc
     2041 ctgggtgatc tcgaagccga agatgatgaa ggcgaatgta gagcgcgagg ccgcgccaaa
     2101 gacggccgcc atcgccgcca gagcaaacgc gcccggctcc agttgcaggc tcggcatggc
     2161 cgagttgagc aggatggcca tcgcgccgcc catggccgcg ctggccatga acaggggggc
     2221 gagcaaaccg cccgatgtgc cggaacccaa tgataccagc agcgccagcg tcttgaagat
     2281 catgaccgac agcaacacgc tgagcgtgag ctggttgttg agaataccgg agatcgtgcc
     2341 atagcccacg cccaggacgc gcggcacgaa gtagccgatg atgcccaggc cgaaaccgcc
     2401 aatgaccggc agccaaaatt tattgacggg tatacggtcg aagaggtctt ccgtcaaata
     2461 gagcaacttg gtgaagccga cggccaccag cccgcagagc agccccagca agatgtagaa
     2521 cggcagggcg cgggggatgc cgaaatcggt gatcgacacc tggaacagcg ggccgcgacc
     2581 caggaacggt atgcgcaccg tcgtggccac caccgtggcg atgaccagcg ggatgaacga
     2641 acgggacttg aactcgaaga gcagcagttc gatggcgatg atgacggcgg cgatgggggt
     2701 gttgaaagtg gccgccatgc cggccgccgc gccacaggcc agcagcacct tgcgctccac
     2761 ggccgtcgtc ggcaggattt gccccaggag cgatcccatc gcgccgccgg tctggatgat
     2821 tggcccttcc gcgccgaacg ggccgcccgt gccgatggcg acggccgtgg cgatgggctt
     2881 gaagatggcc acacgcgggc tgacccggct gcggttttcc agcaccgctt ccatcgcttc
     2941 ggggatgccg tggcccttga tcttctccgt gccgtacttg gccatgaggg ccacgatcag
     3001 cccgccgatg gccggcacga ttagcatcca caggccgatg gggttcgtct ccaggtcggt
     3061 cagctccgtg gccacccgtt ggaagaagac cagattccac agcaagccga tgagcgaaaa
     3121 gagcgcgaag gccagcagcc cggaaatggc gccgatgacg gcggccagga tgctgacgta
     3181 gatcatgcgg aatgatttgg atggttttat gcccatatct tcctgcaagc tcaggttggg
     3241 ttgttcgatc atgattacct cgtttctttt gaatgaattg aaagacgggg tattatatcg
     3301 tagtacgata caatttgtca ggtcgaattt ggaacttgtt cgccgacgcg ataaccgagg
     3361 cagaaaccgg cgatagggtc cgcataagga cgaaagaaga atggctcaac ctatcagcag
     3421 aacggagtat gaaaagctgg cctcattccg ctacgcgctg cgccagtttc tgcgctttag
     3481 cgagacggcc gctcgcgagg ccggtatcac cccccggcag catcaggctc tgttggcaat
     3541 tgccggacat ccgggacgcg aagaggtgat gatgagcgaa ctggctgagc ggctgcaatt
     3601 gcgccatcac accactgtgg aactcatcga ccgcatggcg gcgcaggaac tggtcgttcg
     3661 tgaaccgggc gtggaagacc ggcgctatgt ctatgtctcg ctgacgccgc agggtcacga
     3721 cgtgctggct cgcctctccg tggcccatcg cgaggagctg aagcgcatcg gcccgcagct
     3781 caacttactg ctgcatgaac tctctactgt tggcgaggca gatacgccga ccaacgcatc
     3841 caggtaaaga gcagtcgcgc cacggcgaat tgtttgcctg cccaatcagg aggacgcata
     3901 cgcaaatgcg cccactgcca cccgcgcccc aaaattgcta taatcgatct acgtgacgct
     3961 ccctttcgta gtgggccgcg ccctgacatc cgctatgaca cctgattcta atcctgattg
     4021 aggagttacc catgcccaaa cccattacca gcccgctgac cgaccccgtc tacacccgcg
     4081 caccttattg gaaggacgtg cccgatgaaa agtggatgga ctggcgctgg caaatgtctc
     4141 atcgcctgaa ttccgtcgag gaacttggcc gcgtcatcaa cctgaccgat tccgaacgac
     4201 gcgcgctgac agcccagggc ctttttcgcg tcgatatcac gccttacttc gccagcctca
     4261 tcgaccccga tgacccccat tgccccgttc gtaagcaggt catccccacc gaccgcgaga
     4321 tggtgccttt tgagtcgatg atggaagaca gcctggccga ggacaaacat tcgcccgtgc
     4381 ccggcctcgt ccaccgctac cccgaccgcg tgttgatgct
//
LOCUS       DAOMQG010000291         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8232_length_4419_cov_8.588909, whole genome shotgun sequence.
ACCESSION   DAOMQG010000291 DAOMQG010000000
VERSION     DAOMQG010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8232_length_4419_cov_8.588909"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLC06_13180"
     CDS             <1..807
                     /locus_tag="PLC06_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRO91445.1"
                     /translation="VSLAGAPGAVSAFYRMSIAALVLAIPFFRGLGQTGLPNRRETSI
                     AVLAGLFFAADLFFWNTGILISGATNPTLMGNTAPIWVGIGAMAFFGERPGRLFWIGL
                     LIAIIGAAVILGVDALNDVGLGTFFGILSGMFYSGYFLVVQRSRQKLNTLTSFWLSAV
                     GSVMGLILIARMLGQPLTGYSTMTYVYLLLLSIVVQVGGQYSVAYALGYLPASIVSPT
                     LLLQPVLTGLLAVPILGEKLTPIQILGGVAVLIGVYIVHRGRQVAAQSAD"
     gene            895..2427
                     /locus_tag="PLC06_13185"
     CDS             895..2427
                     /locus_tag="PLC06_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRO91446.1"
                     /translation="MDDNKVKALLNRLHIGGVNPGVCTGPDAWTDTGAPAITSYNPTT
                     GEPIAQVIPATPDAYDHVVTSAEIAFADWRETPAPRRGLVVRDLGVAVREALEPLGEL
                     ITLEMGKIRAEGIGEVQEMIDICDFATGLSRQLYGLTMHSERPKHRMYEQWHPVGPIG
                     VITAFNFPIAVWSWNAAIAAVCGDTVVWKPSELTPLTAVALQHIANQVMADYGLSGIF
                     NLAVGGPEIGRLLANDPRLPLVSFTGSTKVGRTVAQTVAGRLGRTILELGGNNAIVVA
                     ADADLDLATRGILFGAVGTAGQRCTSTRRIIAHRDIAGQLADRLVRAYRQVPVGDPLA
                     EGTLLGPLVTSAAVERMEAAVRQAVSEGGEVLTGGHALPDAGPQFVEPTIIRMPAQTE
                     IVRQETFAPILYLLEYESLDDAMALHNDVPQGLSSAIFTDSLRTAENFLAATGSDCGI
                     ANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKGYMRRQTNTINWSRDLPLAQGIRF
                     ES"
     gene            2455..3591
                     /locus_tag="PLC06_13190"
     CDS             2455..3591
                     /locus_tag="PLC06_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91447.1"
                     /translation="MLRLATIVVLPVLLACRPGLSAAPTDEPITPATVATAEVIVTAP
                     IDIDSSVGVPPNDAPTDEPPVTADTTAPGLPCPLFPADNIWNATIDHLPVHPLSDTYI
                     DTIGRDESFHPDFGSGIWPPDDGGPIGIPYIVVPANQPKVPVDFYYPDESDPGPYPVP
                     PDAPIEGGPNSDGDRHILIAQEGTCLLYEIYDAHPRTDGGWDAGSGAIFDLRSNALRP
                     AGWTSADAAGLPILPGLVRYDEVVAGEITHALRFTAPETQQAFVWPARHFASDLNDTR
                     YPPMGQRFRLRADYDLSDFDPQIQVILRAMQRYGIILADNGSPWYISGTPDERWDNDI
                     LHQLDRLTGDDFEAVDVSSLILDPDSAQVAVRGQSSFIPAIVTR"
     gene            complement(3653..>4419)
                     /locus_tag="PLC06_13195"
     CDS             complement(3653..>4419)
                     /locus_tag="PLC06_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91448.1"
                     /translation="ATQWDVFPGYARMTVQKVMSDIAYYLIYEGTPGGALDLTTDLVT
                     KSDGTTAAANVSWTGDIPGDEWVYFTDPSLDRSLFVIHHQQDEIVDSYTTDAGGKMTI
                     LGFGRAGNGRFLTGLPTEMTFGLVDESSFAAVAAAIDNAYKPLGYTLADAERSPEPST
                     ATPTHTPTEAPTEAPSPTPTETPTSTPTDTPTAIPTDTPAPTDTPTRTPTHTATATET
                     PINTPTATALSAATATFTATRLPGYDLFLPLVVVGP"
BASE COUNT          792 a         1438 c         1295 g          894 t
ORIGIN      
        1 gtcagcctgg ccggcgctcc gggcgcagtc tcggccttct atcggatgtc aatcgccgct
       61 ctcgtcctgg ctatcccgtt cttccgtggc cttgggcaaa cgggcttgcc caaccgccgc
      121 gaaacgtcga tcgccgtgct tgccggcctt ttcttcgccg ccgatttgtt cttctggaac
      181 accggcatcc tgattagtgg cgccaccaat ccgacgctga tgggcaatac cgcgccaatc
      241 tgggtaggta tcggcgcgat ggccttcttc ggcgagcggc cgggacgctt gttctggatc
      301 ggcctgttga tcgctatcat cggcgcggcc gtcattctcg gtgtcgacgc gctaaacgac
      361 gtcggcctgg ggacattctt cggcatcctc tccggcatgt tctacagcgg ttactttctg
      421 gtcgtccagc gcagccggca gaagttgaac acattgactt cgttctggct ctcggccgtc
      481 ggttccgtaa tgggactgat actcatcgcc cggatgctgg gccagcccct gacaggttat
      541 tcaaccatga cctatgtcta tctgctcttg ctgagcatcg tcgtccaggt cggcgggcaa
      601 tacagcgtgg cttacgccct tggctacctg cccgcctcga ttgtctcgcc gacgctgctt
      661 ctgcaaccgg ttctgacggg tcttctagct gtgcccatcc ttggagaaaa actcactccc
      721 attcagatac tcggcggcgt ggccgtgctg atcggggttt atattgtcca tcgaggtcgt
      781 caggttgctg cacaatctgc cgattagttg aaatcattgt aacgctccaa ctttggccct
      841 ccccttgtct ggacatataa tggcgaccac tactcaaagc gaggtcaatc ggaaatggat
      901 gataataaag taaaagcatt actcaatcgc ctccacatcg gcggcgtgaa tcccggtgtc
      961 tgcaccggcc cggatgcctg gaccgacacc ggggcaccag ctatcacttc atataaccca
     1021 acgacaggcg agccaattgc ccaggtcatc ccggccacgc ccgacgccta tgatcacgtt
     1081 gtcacaagcg cggagatcgc cttcgccgac tggcgcgaaa cgcccgcgcc tcgtcgtggc
     1141 cttgtcgtgc gcgacctggg cgtagccgtg cgcgaggctc tggagccgct cggtgagctg
     1201 atcacgctgg agatggggaa gattcgcgcc gagggcatcg gcgaagtgca ggagatgatc
     1261 gacatctgcg acttcgccac cggcctgtca cggcagctct acgggctgac catgcattcg
     1321 gaacggccga agcatcgcat gtacgaacag tggcatcccg tcggccccat tggcgtcatc
     1381 accgctttca acttccccat cgccgtctgg tcgtggaacg cggccatcgc cgccgtctgc
     1441 ggcgacaccg tcgtctggaa accgtcggaa ctaacgcctc tgacggctgt tgccctgcag
     1501 cacatcgcca atcaggtcat ggccgattac ggcctcagcg gcatcttcaa cctggctgtc
     1561 ggcgggccgg agatcggccg cctgctggcc aacgaccctc gtctgcccct tgtatccttc
     1621 accggctcca cgaaagtcgg ccgcacggtc gcccagaccg tcgccgggcg acttggtcgg
     1681 actatcctgg aactaggcgg caacaacgcc atcgtcgttg ccgccgacgc cgacctggac
     1741 ctggccacgc gcggcatcct cttcggcgcc gtcggcacag ccgggcagcg ctgcacctcg
     1801 acgcggcgaa tcatcgccca ccgcgacatc gccggccaac tggccgatcg gctggtgcgc
     1861 gcttatcgtc aggtgcccgt cggcgacccc ttggccgagg ggacattact cggcccgctg
     1921 gtgaccagcg cggccgtcga acggatggaa gcggccgtgc ggcaggctgt ttccgagggg
     1981 ggcgaggtgc tgaccggcgg ccacgctcta cccgatgccg gcccccaatt cgttgaacca
     2041 acgatcattc gtatgcccgc ccagacggag atcgtgcgtc aggagacctt cgcgccgatc
     2101 ctctatctcc tggagtatga gtcactcgac gacgccatgg cgctccacaa cgacgtgccc
     2161 cagggattgt cgagcgccat cttcaccgac agcttgcgca cggccgagaa cttcctggct
     2221 gccacaggct ccgactgcgg tatcgccaac gtcaatattg gcacctcggg cgcggagatc
     2281 ggcggcgcgt tcggcggcga aaaagagacc ggcggcggcc gcgagtctgg ctccgacgcc
     2341 tggaagggct acatgcgccg ccagacgaac acgatcaact ggtccaggga tttgccgctg
     2401 gcccagggaa ttcgcttcga gagctgaacg aataagaggc caacgatgag acgaatgctt
     2461 cgactcgcga caatcgtggt attgccggtg ttactcgcct gtcgtcccgg cctttcggcc
     2521 gcgcccaccg atgaaccgat caccccggcc acggtcgcga cggccgaggt cattgtaact
     2581 gcgccgatcg atatcgattc gtctgtcggt gtgccaccca acgatgcgcc gacagacgag
     2641 ccaccggtca cggctgatac caccgccccc ggcctgcctt gccctctttt cccggccgac
     2701 aacatctgga acgccactat cgaccatctg ccggtacatc ccctgtccga cacctatatc
     2761 gacaccatcg gccgcgacga gagcttccat cccgatttcg gctccggcat ctggccaccc
     2821 gatgacggcg gaccgattgg cattccctac atcgtcgtcc ccgccaacca gccaaaggtg
     2881 cctgttgact tttattaccc cgacgagagc gatccaggcc cctatcccgt tccgcctgac
     2941 gcgcctatcg aaggcggtcc caatagcgac ggcgatcgtc acatcctgat cgcccaggaa
     3001 gggacatgcc tgctctacga gatctacgac gctcacccgc gaaccgacgg tggttgggat
     3061 gccgggtcgg gggcgatctt tgacttgcga tcaaacgcgc tgcgccccgc gggatggaca
     3121 tcggccgacg cggcgggcct gcctatcctg cccggcctcg tgcgctatga tgaggtcgtc
     3181 gcgggagaga tcacccacgc actgcgcttc accgcgcctg agacgcaaca agccttcgtc
     3241 tggcctgccc gccatttcgc gtcggatctg aacgacacgc gttatccgcc gatggggcag
     3301 cgcttccggc tgcgcgccga ctatgatctg agcgacttcg atccacaaat tcaggtcatc
     3361 ctgcgggcca tgcagcgtta cggcatcatt ctcgccgaca atggatcgcc ctggtatatc
     3421 tccggcacgc ctgacgagcg ttgggataat gacatccttc accagctcga ccgattgact
     3481 ggtgacgact tcgaggcggt ggatgtctcc tcgctaattc tcgatcccga ctccgcgcaa
     3541 gtggcagttc gcgggcagtc ttctttcatt cccgccattg tgacacgctg aaatcgcaac
     3601 ctgtcaatga agaaggccgg ttgatttaac cggccttcag gttcgatgac gtctatggac
     3661 cgacaacgac cagcggcaag aacaaatcat agcccggcag ccgagtggcc gtgaatgtcg
     3721 ccgtagccgc agacaacgcc gtagctgtag gcgtattgat cggtgtttca gtggctgtgg
     3781 cggtatgagt tggggttcgt gtcggcgtat ccgttggtgc cggggtatcg gttggtatag
     3841 ccgtaggtgt atccgtcgga gtagacgtgg gcgtctctgt gggcgttggt gacggtgcct
     3901 cagtcggggc ctccgtgggc gtatgggtcg gcgtggctgt cgacggttcg gggctgcgct
     3961 cggcatcggc caacgtgtaa ccgagcggtt tataggcgtt gtcaatcgca gcagccaccg
     4021 ccgcgaacga actctcatct accagaccga acgtcatttc ggttggcagc ccggtcagaa
     4081 agcgaccgtt gcccgcccga ccgaacccca gaattgtcat cttcccaccg gcgtcggttg
     4141 tataggaatc gacgatctca tcctgctgat gatggataac aaagagagag cggtcgagcg
     4201 acggatccgt aaagtagacc cactcgtcgc cgggaatatc ccctgtccag gagacgttag
     4261 ccgctgccgt tgtcccatcc gattttgtga ccaggtcggt cgtcaggtcc agcgcgccgc
     4321 cgggtgtgcc ttcatagatc aggtagtagg cgatatcgct catcaccttc tgcacagtca
     4381 tccgggcata accggggaaa acatcccatt gtgtggccc
//
LOCUS       DAOMQG010000292         4398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8282_length_4398_cov_10.653926, whole genome shotgun sequence.
ACCESSION   DAOMQG010000292 DAOMQG010000000
VERSION     DAOMQG010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8282_length_4398_cov_10.653926"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLC06_13200"
     CDS             <1..592
                     /locus_tag="PLC06_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91449.1"
                     /translation="YVDDQVITLLVYQSPQVEMGFYTQPPPLFAGQPGGLPLQLVNTG
                     RNSTIFGNFTVTAENTELANNAIFVGALEPGGFFPLDAMITPFEPGPLELLLSVSYTD
                     DFSRPAVITQTLSIDVMESMPIEEPPLGPDGLPIEDGGAGGGEFPPGEAPGGEESLLD
                     RLWRFLRGLFGLGSDRSGGDEFAPVAPEMQLEGPPG"
     gene            589..1896
                     /locus_tag="PLC06_13205"
     CDS             589..1896
                     /locus_tag="PLC06_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO91450.1"
                     /translation="MNFFDLLTLIGSNLSRRKGRVALTTIGVIIGTAAVVLLVSLGVG
                     LQQNAEAQLGGIGDLTRIMVYPGMGEGVVEIGPGGVPGQTVPINQAAIDTIAAIPGVA
                     AVIPQDYLQSWAIIRVGRLEGGGQIIGVGTEDMSQLGVTAAQGELLLSRGEVIVGAQV
                     ATNFYDPRARPGQEPPPPPDLYGQTLNIELVKYADDGTEIRKKTRARVVGVLESIQDE
                     PDWSIFMTMSELDGYNAWVSGQRIDRDKNGYNTLIVKVSDVDQVLDVADQITALGFQA
                     WSPQSFVEGISSFYLVLQVAFGSMGAIALLVAAIGIANTMTMAILERTREIGLMKAVG
                     ANNRQVLSIFLGEAAGIGFLGGLGGVLISWGLGQILNVLVLSYMAGQAVQTGGMPPTV
                     AVVTPLWLPLSALGFATLMGLASGLYPALSAATLAPMAALKYE"
     gene            2289..>3040
                     /locus_tag="PLC06_13210"
     CDS             2289..>3040
                     /locus_tag="PLC06_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRO91451.1"
                     /translation="MSKAKVAIVTDSTVNLPAEVIEANNIYVIPQILNWEGRSFLDQI
                     DISTEEFYQRLPNSKDLPKTSQPAPGQFTEHFEKVAEGAESIVAIFVSQALSGTIQSA
                     HLGAEAMGDYPIEIVDSRSTSLGLGLLVTAAARYAAEGHDYKEVAAYVRTLVPRIRVL
                     FVVDTLEYLHKGGRIGGAKRLVGSMLSIKPVLGLEDGVIEPQAQIRTKKKAIQHMLDV
                     VLGEMKGKKNVHAGVIHAVAPEDAAYIVEQVRA"
     assembly_gap    3041..3140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3168..3830)
                     /locus_tag="PLC06_13215"
     CDS             complement(3168..3830)
                     /locus_tag="PLC06_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91452.1"
                     /translation="MEHTIRVLVVDDHAIVRQGLRAMLESKGGIEVIGEAGDGEQAVV
                     QAQLLRPDVILMDLEMPHKSGISATQEIIAAQPDARILILSSFSDDAQIVESMRAGAL
                     GYLLKTAKLDDLVNAIRQINEGETPLNPLVARRLLVSISPPRMEPRLLEVLTDRELMI
                     LPLVVRGMTNKEIGDRLGIATRTVGTHIGNMIRKAEVDNRVQLSMLAVRQGLTSLYAD
                     EN"
     gene            complement(3877..>4398)
                     /locus_tag="PLC06_13220"
     CDS             complement(3877..>4398)
                     /locus_tag="PLC06_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91453.1"
                     /translation="ARGAAESGNMAKLQQHHQSILHMTNQALRELRLLLYEFRTEALA
                     RKGLVDALRERLITVEHRAGISGEVHALDIGSLPMTLEETFYRVAMEALNNALRHAHG
                     DRVDVILMEESGDLVMTIVDNGVGFNRETSAAAGGMGLEGMQKRMGKVGGALTLSSSS
                     GGTWVTARASLKQ"
BASE COUNT          770 a         1336 c         1350 g          842 t
ORIGIN      
        1 ctacgtcgat gaccaggtga tcaccctgct cgtctaccaa tcgccgcagg tggaaatggg
       61 cttctacacc cagccgccgc ccttgttcgc cgggcagccg ggtggcttgc cgctgcagct
      121 ggtgaacacg gggcgcaatt cgaccatctt cggcaacttc accgtgacgg ccgagaacac
      181 cgagttggcc aacaacgcca ttttcgtcgg cgcgctggag ccgggcggtt tcttcccgct
      241 cgacgccatg atcaccccct tcgagccggg gccgctcgag ctgttgctca gcgtcagcta
      301 caccgacgac ttcagccgcc cggcggtcat cacccaaacc ctgtctatcg acgtgatgga
      361 gagcatgccg atcgaggagc cgcccctcgg ccccgacggt ctgcccattg aagacggcgg
      421 cgcgggcggt ggcgagttcc cgccgggaga ggcgcccggc ggcgaagagt cgctgctcga
      481 ccggctgtgg cggttcctgc gcggcctgtt cggcctgggc agtgaccgct ccggcggcga
      541 tgagttcgcg ccggtcgcgc cggagatgca actggaaggc ccaccgggat gaacttcttc
      601 gatttgctga cgctcatcgg ctccaacctg tcccggcgca aggggcgggt ggcgctgacc
      661 accatcgggg tcatcatcgg cacggcggcc gtcgtgctgt tggtgtcgct gggcgtgggg
      721 ctgcagcaaa acgccgaagc ccaactgggt ggcatcggcg atctgacgcg catcatggtc
      781 taccccggca tgggcgaagg ggtggtcgag atcggcccgg gcggcgtgcc ggggcagacc
      841 gtgccgatca accaggccgc catcgacacg atcgccgcca tccccggcgt ggcggccgtc
      901 atcccgcaag attatctcca gagctgggcc atcattcggg tcggccgcct ggaagggggc
      961 gggcagatca tcggcgtcgg cacggaagac atgagtcaat tgggcgtaac ggccgctcag
     1021 ggcgaattgc tgctcagtcg tggagaggtg atcgtcggcg cgcaggtcgc caccaacttc
     1081 tatgatcccc gcgcgcggcc cggccaggag ccaccacccc cgccggacct ctacggccag
     1141 acgctcaaca ttgagctggt caagtatgcc gacgacggca cggagatccg caagaagacc
     1201 cgcgcgcgcg tcgtcggcgt gctggagtcg attcaggacg aaccggactg gtcgatcttc
     1261 atgaccatga gcgagctgga cggatataac gcctgggtat cgggtcaacg catcgaccgc
     1321 gataaaaacg gctacaacac cctgatcgtc aaggtgagtg acgtcgatca ggtgctggac
     1381 gtggccgacc agatcaccgc cctgggcttc caggcgtggt caccgcaatc gttcgtggaa
     1441 ggcatcagca gcttctacct cgtgctgcag gttgcctttg gcagcatggg cgctatcgcc
     1501 ctgctggtgg ccgccatcgg catcgccaac acaatgacga tggccatcct ggagcggacg
     1561 cgtgagatcg gcctgatgaa ggccgtgggg gccaataacc gccaggtgtt gtcgatcttt
     1621 ctgggagagg cggccggtat cggttttctg ggcggcctgg ggggcgtact gatcagctgg
     1681 gggttgggcc aaatcctgaa cgtgctggtg ctcagctaca tggccgggca ggcggttcag
     1741 accgggggca tgccgccgac ggtggccgtc gtcacgccgc tgtggctgcc actgtcggcg
     1801 ctgggattcg ccacgctgat gggactggcg tcggggctgt atcctgcgct cagcgcggct
     1861 actctggctc ccatggcggc gctgaaatat gagtgagaca aaccgggcgg gacagggcgg
     1921 catccaggtg tggcacaatt tgtaggggcg ggtttgggtg acaggttgcc gtcgcacccg
     1981 tccctactga atcaatcaaa agcccgacag ggacttcctg ccgggcttta tttcgactgg
     2041 ttaccacccc gtcaatgaat cctccaatga cggtacatcc ctcaatgtac ctattagagt
     2101 acacgttttg cggcagggcg taaatgggga ttctgcggat ttttcctacg tattttattt
     2161 agatcgttgc gggtgtttcc ccctattcct gcctcaattg acgattccgg ctgcctgttc
     2221 tacaatcttc acgctcgtct cgcacgggta gaaatcatca tcttttattc caaggagaaa
     2281 ggggcgctat gtccaaagcc aaagttgcca tcgtgaccga tagcacggtc aatctaccgg
     2341 cagaggtcat cgaggcaaac aatatctatg tcatcccgca gattctgaat tgggagggcc
     2401 ggagcttcct cgatcagatc gatatcagca ccgaggagtt ctaccagcgg ctgcccaatt
     2461 caaaagatct gccgaagacg tcccagcccg cgccggggca gttcaccgaa cacttcgaga
     2521 aagtagccga gggcgcggag agcatcgtgg cgatcttcgt gtcgcaggcg ttgagcggca
     2581 ccatccagtc ggctcacctg ggcgcggaag cgatgggcga ttatccgatt gaaattgtcg
     2641 actcgcgctc cacatcgctc ggactggggt tgctggtgac ggccgccgcg cgctatgcgg
     2701 ctgagggcca cgactacaag gaagtcgccg cctatgtgcg gacgctggtg ccgcgcattc
     2761 gcgtcctttt cgtcgtcgac acgctggaat atctccacaa gggcggccgt atcggtgggg
     2821 ccaagcgcct cgtcggctcc atgctctcga tcaagcccgt attgggtctg gaagacggtg
     2881 tgattgagcc gcaggcgcag attcgcacca agaaaaaggc gattcaacac atgctcgatg
     2941 tggtgctggg cgagatgaag ggcaagaaga acgtccacgc cggcgtcatc catgccgtcg
     3001 cgccggaaga tgccgcctac atcgtggaac aggtgcgggc nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn tcgggcctgg ctttgccccg cgctgactca attctcgtcc
     3181 gcgtagagcg aagtcaggcc ctgtcgcacc gccagcatcg acaactgcac gcgattatcc
     3241 acctcggcct tgcgtatcat gttgccgatg tgggtgccga ccgtgcgtgt ggcgattccc
     3301 aggcggtcgc cgatctcctt gttggtcatc ccgcgcacga ccaggggcag gatcatcaat
     3361 tcccgatcgg tcaacacctc cagcaggcgc ggctccatcc gtggcggtga gatggaaacc
     3421 agcaggcggc gagccaccaa agggttgagg ggggtctcgc cttcgttaat ctggcgaatg
     3481 gcattcacca gatcgtccag cttggccgtt ttcagcagat agcccaacgc cccagcgcgc
     3541 atggattcca caatctgcgc gtcgtcgctg aagctggaaa ggatgaggat gcgcgcgtcg
     3601 ggctgcgcgg cgatgatctc ctgagtcgcg ctgatgccgc ttttgtgcgg catttccagg
     3661 tccatcagga tcacgtcggg cctcaacagt tgcgcctgaa cgacggcctg ttcgccgtcg
     3721 cccgcctcgc caatcacctc gatccccccc ttgctctcca gcatggcgcg caaaccctgg
     3781 cgcacaatgg cgtggtcgtc tactaccaga actcgtatcg tatgttccat cttttacccc
     3841 tcctgattcg ctgatggatt tgccgcccct cacggcttat tgcttcagag acgctctggc
     3901 ggtcacccag gtgccgccgg agcttgagga cagggtgagc gcgccgccca ccttgcccat
     3961 tcgtttctgc attccctcca atcccatgcc tcccgccgca gcggaggttt cacggttgaa
     4021 cccgaccccg ttatccacga tcgtcatgac cagatcgccg ctttcctcca tcaaaataac
     4081 gtccaccctg tccccgtggg catggcgcaa ggcgttgttc agggcttcca tggccacgcg
     4141 atagaatgtc tcttcgaggg tcatcggcag gctgccgatg tcgagcgcgt ggacttcgcc
     4201 ggaaatgccg gcgcgatgct cgacggttat caggcgctcg cgcagcgcat cgaccagccc
     4261 cttgcgagcc agcgcctcgg tgcggaactc gtacagcaac aatcgaagtt cgcgcagggc
     4321 ctgattggtc atgtgcagga tggattgatg gtgctgttgc agctttgcca tgttgccgga
     4381 ttcggccgcg ccgcgggc
//
LOCUS       DAOMQG010000293         4392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8296_length_4392_cov_7.580586, whole genome shotgun sequence.
ACCESSION   DAOMQG010000293 DAOMQG010000000
VERSION     DAOMQG010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8296_length_4392_cov_7.580586"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLC06_13225"
     CDS             <1..246
                     /locus_tag="PLC06_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91454.1"
                     /translation="AGRLVPAAEFFVSPGYCTERIYVFLGLDLEPIAADPDADEEIER
                     VELTLPEVRRMLDERQFHDSKTIIGLRELLAWLEREK"
     gene            complement(267..1559)
                     /locus_tag="PLC06_13230"
     CDS             complement(267..1559)
                     /locus_tag="PLC06_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HRO91455.1"
                     /translation="MTEPQSILQLKQVSQEYGSGERRFLAVDNVELTLKEGEYVALLG
                     PSGCGKSTLLRIIAGLQSATRGEVLYRGEHLRGVNPYTTIVFQTFALFPWLTVLENVN
                     LALQAKGIPLENRAVEAIDRVGLDGFESAYPRELSGGMRQKVGFARAIVSEPELLCLD
                     EPFSALDVLSAESLRRDLLELWNEGLIPTKAILMVSHNIEEAVFMADRIVVMDKNPGR
                     IVADLTIPLAHPRQRKSAEFQALVDQVYATMAGQTQPEHLEVGTAPGEPGLTRSLPTV
                     VINDLAGLLEALLHEPEFQADIYRLASDLQLNSDQLLSLVEAAELLGFATVKEGDIVL
                     TPLGETFAEASILARKEIFATRIRRLPIFKWIMALLRAADQQRLGWDVILAALGLEFP
                     PQEAERQLDLIVDWGRYAELLVYDDDTAILQLEAPQPA"
     gene            complement(1564..3270)
                     /locus_tag="PLC06_13235"
     CDS             complement(1564..3270)
                     /locus_tag="PLC06_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRO91456.1"
                     /translation="MIFGSTTPRRAFGPGDILALLIVLTVLYVGTRLAFNAPTVIAGP
                     DINLSPSALPWYAALSLGRMVAAYVLSLIFTLFYGRVAAYNARAEQVMIPLLDVLQSV
                     PILSFLPVVLLGLSAILPVSVAAELAAIILIFTSQVWNLTFAWFQSLTTIPKELREAS
                     KIFRLNTWMQFKTLELPFAAISLVWNSMMSWAGGWFFLMAAEIFTVGQQDFRLPGLGA
                     YLHEAANQSDFTAIGWGIATLVLVVVLLDQFVWQPLLAWSDRFKVEMVEDSDGPTSWF
                     YDLWRRARLAEWVRTRLIGPTMERFDNWTVRRFPMKSTAPPAEPERPWWQLALGIAAG
                     GVLIYGGYRAAALLITVPLPEWEEIALGLGATLLRVFAALLIALAWTVPVGVIIGTNR
                     RLAAVLQPMTQIAASVPATALFPVLLLFVLGLPAGLQLAAVLLMLMGTQWYLLFNVIA
                     GASAIPQDLKYTTAMLQLGRRERWRTLFLPALFPYLVTGAITAGGGAWNASIVAEYVQ
                     FNGQTLTVTGIGATIATATAQGNYALLLAATLSMVLAVVLINRLLWRRLYRLAEEHYR
                     ME"
     gene            complement(3460..4266)
                     /locus_tag="PLC06_13240"
     CDS             complement(3460..4266)
                     /locus_tag="PLC06_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO91457.1"
                     /translation="MKSIAGTAELNATTFHYEMAGIGPPVVLIHAGIADSRMWEGQFA
                     PLSRWFRVVRYDMRGFGLTPPAEGVFSHTGDLAALLRHLGIARVSLVGCSMGSKTALD
                     FALAYPEQVDRLVLTSPAVSGLPYDGPPPPQAEELDAADAAGDLARVNELEMQIWIDG
                     PYRTPDDVDPGLRALVAEMNAIALANEGIGEELSPSRPAAGRVGDVNVPVLVINGGLD
                     ALRTRAAADWLAAELPDTRRIVMSGLTHLPNMERPEEYNRLVVEFLQGYL"
BASE COUNT          813 a         1450 c         1405 g          724 t
ORIGIN      
        1 gccgggcggc tcgtgcccgc ggccgagttc ttcgtctccc ccggctactg caccgagcgc
       61 atctacgtct tcctcggcct cgatctggag ccgattgcgg ccgatcccga cgccgatgag
      121 gagatcgagc gcgtggagct gacgctgccc gaagtgcgcc ggatgctcga cgagcgccaa
      181 ttccacgaca gcaagacgat catcggcctg cgcgagctgc tggcctggct ggagcgggag
      241 aagtagctcc tgcctccgct atgaccctac gcgggttgcg gggcctccag ctgcaggatg
      301 gcggtgtcgt cgtcatagac caacagctcg gcataccgcc cccagtcgac gatcaaatcc
      361 agctgccgct ccgcttcctg cggggggaac tccagcccca gcgccgccag gatcacatcc
      421 cagcccagcc gctgttggtc ggccgcgcgc agtagagcca tgatccactt gaagatcggc
      481 aggcgacgga tgcgcgtggc gaatatctcc ttgcgggcca aaatagaagc ctcggcgaac
      541 gtctcgccca ggggcgtcag gacgatatcg ccctccttca cggtggcgaa ccccagcagt
      601 tcggccgctt ccacgaggct gagcaactgg tcggagttca attgcaggtc gctcgccaga
      661 cggtagatgt cggcctggaa ctccggctca tggagcagcg cttccagcag cccggccagg
      721 tcgttgatga ccacagtggg caggctgcgc gtcagtcccg gttcgccggg ggcggtgccg
      781 acctccaggt gttccggctg ggtctgcccg gccatcgtgg cgtaaacctg gtcgaccagc
      841 gcctggaact cggccgattt acgctggcgc gggtgggcca gcgggatggt cagatcggcc
      901 acgattcgcc ccgggttctt gtccatgacg acgattcggt cggccatgaa gaccgcttcc
      961 tcgatgttgt ggctgaccat caaaatcgct ttcgtgggga tcaacccctc gttccacaac
     1021 tccagcaagt cgcggcgcaa cgactcggcg ctgagcacgt cgagcgccga aaaaggctca
     1081 tcgagacaca gaagctccgg ctcgctgacg atggcgcggg caaagccgac cttttggcgc
     1141 attccgcccg acagttcgcg gggataggcg ctctcgaaac cgtcgaggcc gacgcgatcg
     1201 atcgcttcca cggcgcgatt ttctaggggg atgcctttgg cctggagcgc caggttgacg
     1261 ttctccagca cagttagcca gggaaagagg gcaaaggtct ggaaaacgat ggtggtatac
     1321 gggttgacgc cgcgcaggtg ctccccgcga tacagcacct cgccccgggt ggccgattgc
     1381 agcccggcga tgatgcgcaa cagggtgctc ttgccgcagc ccgacggccc cagcagggcc
     1441 acgtactccc cctctttcag ggtcagttcc acgttatcga cagcgagaaa ccgccgctcg
     1501 ccgctgccgt actcctgcga tacctgcttg agttgcagta tggattgtgg ctcggtcatc
     1561 gtcttactcc atccgatagt gttcctcggc caggcggtag agtcgccgcc acaggagccg
     1621 gttgatcagc accaccgcca gcaccatgct caacgtggcc gccagcagca gggcgtagtt
     1681 tccctgcgcc gtggccgtgg cgatggtggc gccgatgccg gtgacggtca gggtttggcc
     1741 gttgaactgc acgtattcgg ccacgatgct ggcgttccac gcgccgccgc cggcggtgat
     1801 ggcccccgtc accagatagg ggaacagcgc cggcagaaac agcgtgcgcc accgctcgcg
     1861 ccgccccagt tgcagcatgg cggtggtgta cttcaaatcc tgcgggatgg cgctggctcc
     1921 ggcgatcaca ttgaacagca gataccactg cgtgcccatg agcatcagca gcaccgccgc
     1981 caattgcaag ccggctggca gccccagcac gaacagcaac aacaccggaa acagcgccgt
     2041 ggccgggacg gaagcggcga tctgggtcat cggctgcagg acggccgcca gccgccggtt
     2101 ggtgccgata atcaccccca ccggcaccgt ccaggccagc gcgatgagca gcgcggcgaa
     2161 aacgcgcagc aaggtcgccc ccagccccag cgcgatctct tcccactccg gcaagggcac
     2221 ggtgatgagc agcgcggcgg cgcgatagcc gccatagatc agcacgcccc cggcggcaat
     2281 gcccagggca agctgccacc agggtcgctc cggctccgcc ggtggggcgg tcgatttcat
     2341 ggggaagcgg cgcaccgtcc agttatcgaa gcgctccatc gtcggcccga tgaggcgcgt
     2401 ccgcacccat tccgccaggc gcgcgcggcg ccagaggtcg tagaaccatg acgttggccc
     2461 gtcgctgtct tcgaccatct ccaccttgaa ccggtcggac caggccagca gcggctgcca
     2521 gacgaactgg tccagcagca cgaccaccag caccagcgtg gcgatgcccc agccaatggc
     2581 cgtgaagtcg ctttggttgg ccgcctcatg cagataggcc cccagccccg gcaggcgaaa
     2641 gtcctgttgg ccgacggtga agatctcggc ggccatgagg aagaaccagc cgccggccca
     2701 gctcatcatg ctgttccaca ccagactgat ggcggcaaaa ggcagctcca gcgtcttgaa
     2761 ctgcatccag gtgttcaggc ggaaaatctt gctcgcctcg cgcagctcct tggggatggt
     2821 cgtcagcgac tggaaccagg caaaggtgag attccacacc tggctggtga agatcaggat
     2881 gatggccgcc agctcggccg ccacactaac cggcaggatg gcgctgaggc caagcagcac
     2941 caccggcaga aaggagagga tgggcacgct ctgcaacacg tccagcaggg ggatcatcac
     3001 ctgctcggcg cgggcgttat aggcggccac gcggccgtag aacagcgtga atatcagcga
     3061 cagcacatag gcggccacca tgcgccccag cgacagcgcc gcgtaccagg gcagcgcgct
     3121 gggtgacaga ttgatgtccg gtccggcgat caccgtcggc gcgttgaatg ccagccgagt
     3181 tccgacgtag agcaccgtca gcacgatcag caaggccagg atatcgcccg gcccgaaagc
     3241 ccgccggggt gtggttgagc caaagatcat atgccctcac aaccatcgcc cgacaacctg
     3301 aatacagccg cgccgagcga caggcattat agccccatta tcgtttatgg gtatagtggc
     3361 tagtcatccc cagccaggtc tttaacagca gcatgtacgg gcgggacaac cggcgcgaga
     3421 gcctttgtcc agcttgtggt gcggacagga atgtctgcgc tacaggtagc cttgcaggaa
     3481 ttcaaccacc agccggttgt attcttccgg ccgctccatg ttgggcaggt gcgtcaggcc
     3541 ggacatgacg atcctgcgcg tgtcgggcag ttcggcggcc agccagtcgg ccgcggcccg
     3601 cgtccggagc gcatccagtc cgccgttgat aaccagtacc ggtacgttga cgtcgcccac
     3661 ccgtccggcg gccgggcgtg acggcgacaa ctcctcgccg atgccttcat tcgccaaagc
     3721 gattgcgttc atctcggcca ccagggcgcg caggccgggg tctacgtcgt cgggtgtgcg
     3781 atacggaccg tcgatccaga tttgcatctc cagctcgttg acgcgcgcca gatcgcccgc
     3841 cgcatcggcc gcgtccagct cctcggcctg cggcggcggc gggccgtcat atggcaggcc
     3901 gctgacggct ggtgaggtca gcaccagccg atcgacctgc tctggatagg ccagcgcgaa
     3961 atcgagcgcc gtcttgctgc ccatcgagca gccgacgagc gacacccgcg cgatgcccag
     4021 atggcgcagc agcgcggcca ggtcgcccgt gtgggagaat accccctcgg ccggaggagt
     4081 caggccaaag ccgcgcatgt cgtagcggac aacgcggaac caccgggata gtggcgcaaa
     4141 ttggccttcc cacatgcgac tatcggcgat gccggcgtgg atcaaaacaa ccggcgggcc
     4201 gatgccggcc atctcgtagt ggaaagtcgt ggcgttcagc tcggccgtgc cggctattga
     4261 tttcatgggt catactccgt ggaaatgcgc ggtatttcgc gtcgatgcgg gcctcaatgc
     4321 gcgcgcgccg ttctccacat acagaacggc gtcgccgaca cgagatatta tagacgctga
     4381 ttggatcttt tg
//
LOCUS       DAOMQG010000294         4387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8314_length_4387_cov_5.695522, whole genome shotgun sequence.
ACCESSION   DAOMQG010000294 DAOMQG010000000
VERSION     DAOMQG010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8314_length_4387_cov_5.695522"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..259)
                     /locus_tag="PLC06_13245"
                     /pseudo
     CDS             complement(80..259)
                     /locus_tag="PLC06_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
     gene            complement(281..655)
                     /locus_tag="PLC06_13250"
     CDS             complement(281..655)
                     /locus_tag="PLC06_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRO91458.1"
                     /translation="MTSFLDLWHPIVFDRDLVALRELLAEDVVFRSPYVWRPYHGRQA
                     AWLILSTVLDVFQEFTYHRELIDGDNLALEFSARVGDLSLKGIDLIRLDSDGRIVEFE
                     VFIRPFNGLQALGEEMSRRLAG"
     gene            complement(811..1428)
                     /locus_tag="PLC06_13255"
     CDS             complement(811..1428)
                     /locus_tag="PLC06_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91459.1"
                     /translation="MNDQIIRPEPGARYAFDVSLEAAETYIRAARHQMKHDRAAGMAS
                     FDDIFRSCEPPRAMNGRFGGELLAVDFVPGLNQYAAWVQRRFRPWLGKVLDSETATGD
                     NIFDRAADPWLRLMMPLYRRRLPDTPATFRAVKFRTMTAPSLGDPDMVVLRLDYDLPP
                     NPAHTVRRIVDEVVELADGYYFGRAFVHLWWGSWVRVAYFSLRAT"
     gene            complement(1689..1958)
                     /locus_tag="PLC06_13260"
     CDS             complement(1689..1958)
                     /locus_tag="PLC06_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HRO91460.1"
                     /translation="MIYVMYQHEVADYAKWRAVYDSKVDLRRSAGQRNERVFHGVDNP
                     NLVTILVGWDSVEGANSWGNDPRLQTAMKDAGVLSRPTVTFLNED"
     gene            2207..4318
                     /locus_tag="PLC06_13265"
     CDS             2207..4318
                     /locus_tag="PLC06_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91461.1"
                     /translation="MSESLSLHHRLEQARRRRFVGRESERELLRVTLSAAELPFFVLH
                     VFGPGGIGKTSLLHEFAHIGREAGLFVATLDGRQIDPSPDTFLRALCVALNLPPGTDP
                     VEHLAANPGRMMILVDTYELLFPLDGWMRQQFLPSLRDDIFVVLAGRNPPATAWRIDP
                     GWQSLMRILPLRNLGPEESRAYLELRGVPADQFDAALDFTHGHALALSLVADVYDHDP
                     NAGFRPETAPDVIRTLLDELIRHVPGAHHRAALEAAALVHLTTESLLATLLAQNDAHE
                     LFDWLRGLSFMDVDRRGIYPHDLAREALAADLRWRNPDRFAELHQRARNYYMKRLGSE
                     TGQDQRFLLAEFLYLHRDNPSIRPYFLWQESGTVFTDAMRPDDIEPLMAMVAAHEGRE
                     SAALAAHWLARQPRNVSVLREAAGAPVAFVSRVNLEATTAADRQLDPAIAAAWEVIHR
                     KPLRAGEMALYFRFWMGKESYQSVSSEQSRLFLVIVQDYLMTPGLAFTFLPCADANFW
                     SAMFQYADLERFPEADFTVGSRRYGVYGHDWRETPPPAWLDLMAEREMNLSPPRPRPR
                     SAAEQLIVLSEADFAVAVRDALRDYTDTARLAHNPLNRSRLVGVSAPPGAATADPAAP
                     PVAELRKILRETAESLQESLRLAKLYRALYHTYFQPAVTQEQAAELLDLPFSTYRRHL
                     RAGIEEMTARLWAREIGNLGG"
BASE COUNT          796 a         1420 c         1395 g          776 t
ORIGIN      
        1 cgagttcggc ggcgaagcgg gcataggcgg cttccagcgc ggcggccgag accatcccgt
       61 cgcccaccgg ctcgccgtcg tcgcaccgca cgcaatccag cggcgcgccc aggaagcggc
      121 gacggccttc ctcggtcagc acccacggcc gattgacgaa cggcgggttg tggcgcacgt
      181 gttgacgatg gccacaggcc agaatggccc gccagtcgcc cagctcgtcc tgatcgtagc
      241 cgatgatggg ttgttccata gagcagcggg gcgcggagcg ctatccggcc aggcggcggg
      301 acatctcctc gcccagcgcc tgcagcccgt tgaagggccg gatgaacact tcgaactcga
      361 cgatgcggcc gtcgctgtcg aggcggatga ggtcgatgcc cttcagcgag agatcgccga
      421 cgcgggcgct gaactccagc gccagattgt cgccgtcaat cagttcgcgg tggtaggtga
      481 actcctggaa gacgtcgagg acggtgctga ggatgagcca tgccgcctgc cggccatgat
      541 acggccgcca gacataggga gagcggaaga cgacgtcttc ggccagcaat tcccgaagcg
      601 cgacgaggtc gcggtcgaat acgatggggt gccagaggtc gaggaaggaa gtcataataa
      661 tcaggaatca ggaatcgtga attggggcgg cgatggttat tgtgatggat ttcaccaaat
      721 agtaattcag gatcgcgttt ggggcaacag gagggggaat caggagttat gagggacgaa
      781 cgacgagtga tgaccgcagc ttccccggtg tcaggtcgct ctcaggctga aataggccac
      841 ccgaacccac gacccccacc acagatgaac gaacgcgcgg ccgaaatagt agccgtcggc
      901 caattccacc acctcatcca cgatccggcg gacggtgtgg gccgggttgg gcggcaggtc
      961 gtaatcgagt ctgagcacca ccatgtcggg gtcgcccagc gatggagccg tcatggtgcg
     1021 aaatttgacg gcgcggaagg tggcgggggt gtcgggaagt cgccggcgat agagcggcat
     1081 catgagccgc agccacggat cggccgcccg atcgaagatg ttgtcgccgg tggccgtctc
     1141 cgaatccagc accttgccca gccaggggcg aaaccggcgc tgaacccagg ccgcatattg
     1201 attgaggccc ggcacgaaat cgaccgccag aagctcgccc ccgaagcggc cgttcattgc
     1261 ccgcggcggc tcgcaggaac ggaatatgtc gtcgaacgag gccatccccg cggcgcggtc
     1321 gtgcttcatc tggtgccggg cggcgcgaat gtaggtctcg gccgcttcca atgagacgtc
     1381 gaacgcatat cgggcgccgg gttcgggtcg aatgatctgg tcgttcatgt gctgaaggga
     1441 gcctggcgtg gccggcaaca aaaaaccggc ttcggcgatg ccaaccggcg ccggaacaga
     1501 ctatacagcc tgaatccagt gacggacggc cgccggagcc ggtcgaatag gttcaggaag
     1561 ctcgctcgtc ggcggaaaac ccatcccgac gcttggggca gacagcatga tgtcgccgcg
     1621 gcaaacgcgg cgggttggtt gaccgaccgg cgagtgagtt ggtgaaaccg gcgtcatcct
     1681 gggtgcgctc agtcttcgtt gaggaacgta acagtcggcc ggctcagcac gccggcatcc
     1741 ttcatggccg tctggagccg cgggtcattc ccccaggaat tcgcgccctc gacgctgtcc
     1801 cagccgacca ggatcgtcac gagattgggg ttgtccacgc catgaaagac gcgttcgttc
     1861 cgctggccgg ccgaacggcg caaatctacc ttcgaatcgt acaccgcgcg ccacttggcg
     1921 taatcagcca cttcatgttg atacataacg taaatcatca gcttatctcc attgtctgca
     1981 aggatgtgtc gtgatcatcg atcacgctcg tcgttccccc gtagggttcg gaaaccgggt
     2041 ttcttgtccc tgagggaatt acaatcagca tcgtagcgcg ccactaacgt gagtaacagt
     2101 ccaattatta ctcatttttt ggtcaaaaaa gccgcgatcg gccggacgaa aattcacccg
     2161 cgaagggatt gacaaccgtg caatctggcc ccaaaattat gggcagatga gtgagagcct
     2221 atcgttgcac catcgtctgg aacaggctcg ccgccgtcga ttcgtcggtc gtgaatccga
     2281 gcgcgagttg cttcgcgtca ccctgtcggc cgcggaattg cccttttttg tgctccatgt
     2341 cttcggcccg ggcggcatcg gcaagaccag cctgctgcac gaattcgccc acatcggccg
     2401 cgaagcgggg ctgtttgtcg ccactctcga cggccgccag atcgacccct cgcccgacac
     2461 cttcctgcgc gccctgtgcg tcgcgctcaa cctgccgccg ggcaccgatc cggtcgagca
     2521 tctggcggcc aaccccggcc ggatgatgat cctcgtcgat acctatgagc tactgttccc
     2581 gctcgacggc tggatgagac agcaatttct gccgagcctg cgcgacgata tcttcgtcgt
     2641 gctggccggg cgaaatccgc cggccaccgc ctggcgcatc gatcccggct ggcaatcgct
     2701 catgcgtatc ctgccgctgc gcaatctggg gccggaggag agccgcgcct atctggagct
     2761 gcgcggcgtc ccggccgatc agttcgacgc cgccctcgac ttcacccacg gccacgcgct
     2821 ggccctgtcg ctggtagcgg atgtctatga ccatgacccc aacgccgggt tcagacctga
     2881 gaccgcgccg gacgtgatac gcacgctgct cgacgagctg atccgccatg tgcccggcgc
     2941 gcatcaccgc gccgcgctgg aggccgcggc gctggtccat ctgaccaccg aatcgctgct
     3001 ggcgactctg ctggcccaaa atgacgcgca cgaactgttc gattggctgc gcggtttgtc
     3061 gttcatggat gtcgaccgac gaggcatcta cccccacgac ctggcgcgcg aggcgctggc
     3121 agccgatttg cgctggcgta accccgatcg ctttgccgaa ttgcaccaac gcgcgcgaaa
     3181 ctactatatg aagcgcctgg ggagcgagac ggggcaagat cagcgctttt tactggcgga
     3241 attcctctat ctccaccgcg ataacccttc catccggccg tatttcctgt ggcaggaatc
     3301 gggcacggtg ttcaccgacg ccatgcgccc cgacgacatc gagcctctga tggcgatggt
     3361 ggccgcccac gaagggcgcg aatcggccgc tctggccgcc cattggctcg cccgacaacc
     3421 ccgcaacgtg agcgtgctgc gcgaggcggc cggcgcgccg gtggcttttg tcagccgcgt
     3481 caacctggag gcgaccacgg ccgccgatcg ccaactcgac ccggccatcg ccgccgcctg
     3541 ggaggtgatc catcgcaagc cgctgcgggc cggcgagatg gcgctctatt ttcgcttctg
     3601 gatgggcaag gagtcgtacc agtcggtctc gtccgaacaa agccgcctct ttctggtcat
     3661 cgtgcaggat tacctgatga cgccggggct ggccttcacc tttttgccct gtgccgacgc
     3721 caatttctgg tcggcgatgt tccagtacgc cgacctggag cgcttcccgg aggccgattt
     3781 caccgtgggc agccgccgct acggcgttta tggccacgat tggcgcgaga cgcccccacc
     3841 ggcctggctc gacctgatgg ccgagcggga gatgaacctg tcgccgccgc gcccgcgccc
     3901 ccgctcggcg gccgagcagc tgattgtcct gagcgaggcc gacttcgccg ttgccgtccg
     3961 cgatgcgctg cgcgactaca ccgacaccgc ccggctggcc cataaccccc tcaatcgctc
     4021 gcgtctggtt ggggtttcgg cccctccggg cgcggcgacc gccgatccgg ctgcgccacc
     4081 cgtcgcggag ctgcgcaaaa ttctgcgcga gacggccgaa tcgctgcagg aatcactccg
     4141 cctggccaag ctctatcgcg ccctctacca cacctatttt caaccggcgg tgacgcagga
     4201 gcaagcggcc gaactgctcg acctgccttt cagcacctat cgacgccatc tgcgcgcggg
     4261 catcgaggag atgacggccc gcctctgggc gcgcgagatc ggcaatctgg ggggataggt
     4321 cgtgctcgtt atggacgaca ggagtcgtcg gacgacagga gtcgtcggac gacaggagtc
     4381 gtcggac
//
LOCUS       DAOMQG010000295         4380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8325_length_4380_cov_10.283699, whole genome shotgun sequence.
ACCESSION   DAOMQG010000295 DAOMQG010000000
VERSION     DAOMQG010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8325_length_4380_cov_10.283699"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLC06_13270"
     CDS             complement(<1..340)
                     /locus_tag="PLC06_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91462.1"
                     /translation="MKQSRLIVLIALAFLAACRPALGEIAPTIAPTAGAPTADVPTGA
                     PATAALTPAEPVTPTDEPSTPPATDATAPLFDIDPFDRSPFAAGLISDEVGGDALPGA
                     PVYHMAITIDP"
     gene            complement(556..1548)
                     /locus_tag="PLC06_13275"
     CDS             complement(556..1548)
                     /locus_tag="PLC06_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91463.1"
                     /translation="MVWILFVASSAVVVLAAIKLAEYSDVIAVRTGLGGLFVGTIFLA
                     GATSLPELTASISAFRTGALNLAAGNFFGSNMTNIALLAIVDLTHRQAPLLRRVAISH
                     SLTAALATILMLVAVLAMVADIDLAIGWVGVDSLLLIALYFAGIWVVQRENQATAGPP
                     PAVMAPAEGFPSLRRGIIGFIVAGAILIAAVPQLVSSSVGIAEATGLGTGFVGTALLS
                     FVTSLPELVAALAAVRLGAFDLAVGNLLGSSVFNMFAMAISDFFLLDGPFLSLIDSNF
                     VLVGLLGILLTNMALVGNLARVERKFLFFELDSLVILLVYLLGMYLLFLRGVGV"
     gene            complement(1552..1923)
                     /locus_tag="PLC06_13280"
     CDS             complement(1552..1923)
                     /locus_tag="PLC06_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91464.1"
                     /translation="MYMLIMVLDNVAHREEVLASWERAGIRGVTILESTGLHRVLLRH
                     RPDVAYSGFGRIFGNTEAGHNTLFAVVDDMAVIEAAVAATERIVGKLDGPNTGIMFAL
                     PVSRVWGLPGNPSTAKEGRER"
     gene            complement(1980..2405)
                     /locus_tag="PLC06_13285"
     CDS             complement(1980..2405)
                     /locus_tag="PLC06_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91465.1"
                     /translation="MIDITLDRPEASSAALTFSDGHALIDMTDAHGINGLTATLIDGE
                     WPEQITIHLRLRGLERLEIHYGPYTIATGLSSNNSPDPPPALHVADENEKVKEIPDPD
                     SIYIPDISRTDDGFDITLPAHFFQGKYSAFSMQWIDFYR"
     gene            complement(2505..3467)
                     /locus_tag="PLC06_13290"
     CDS             complement(2505..3467)
                     /locus_tag="PLC06_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO91466.1"
                     /translation="MKLIIQIPCYNEAETLPAALVELPAAIPGIDCIETLIIDDGSDD
                     GTAAVAEALGVNHIIRHQRNLGLAQAFQTGIDACLRLGADIIVNTDADNQYPGRYIPD
                     LVGPVLAGQADMVIGDRQVQTIPHFSPMKKRMLALGSWMVRNVSGVDVADPVSGFRAY
                     SREAALRFTILTRYSYTLETIIQAGKSGLGIVSVPVTTNPPTRPSRLQRSMWHFIKAQ
                     AGTILRLYAFYEPLRTFSYIAAPFLLAGIALWARFMFNYLTGESGVGRFVQSLTLGTG
                     LLLVGTLIVLFGIQADISGKHRQLTQEMLYRLKKMELNRPIGSE"
     gene            complement(3509..3883)
                     /locus_tag="PLC06_13295"
     CDS             complement(3509..3883)
                     /locus_tag="PLC06_13295"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRO91467.1"
                     /translation="MSTKGPDFYRQVYAVVRRIPRGRVTSYGRVAAMLGRPNAARAVG
                     YALSALGSKRDNEPPVPWQRVINHAGRISTPDMDGGASRQKELLQAEGVVVSDDLRVD
                     LRVYLWQGLHLVELDDIIAGRD"
     gene            4052..>4380
                     /locus_tag="PLC06_13300"
     CDS             4052..>4380
                     /locus_tag="PLC06_13300"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO91468.1"
                     /translation="MTKFDNLDLIVRDVGAAVDFFERVVGLEARVVDEHFAELESGEV
                     TIMLSPTAMIPVKPASGMILHFRVENVQKALERANAYGATVLMEPMLTDWGTESLLIA
                     GPEEIVI"
BASE COUNT          844 a         1306 c         1449 g          781 t
ORIGIN      
        1 acgggtcgat ggtgatggcc atgtgataga ccggcgcgcc gggcagggcg tcgccaccga
       61 cttcatcgct gatcagtccg gcggcgaagg ggctgcggtc gaacgggtcg atgtcgaaga
      121 gcggggcggt cgcgtccgtt gcgggcggtg tggatggttc atccgtgggc gtgacgggtt
      181 ctgccggtgt gagcgctgcc gtggcgggcg ctcctgtggg cacgtcagcc gtgggtgcgc
      241 cggccgtagg tgcgatggtg ggcgcaatct cgcccagtgc aggacgacaa gcagcaagaa
      301 aagcaagagc gatcaggacg atgaggcgcg attgtttcat ggcccccaat tgtcatgctg
      361 gtgacgaggg aaggcaaaga ttctaagcaa gtgacgaatg acgagtgacg agtgaagacg
      421 aagtacgaaa tacgaagtag aaagagcgcg ctcttgccgt ggtcgcgcga cgggagtcgc
      481 gatggtccgt ggtcacgggt ctgtcgtcgc gcgacaggag tcgcgtcgtc tgtcgtcggc
      541 ggacaagagt ccgctctaaa cccccacccc acgcaaaaac aacaaataca tgcccaacag
      601 atagaccagc aggatgacca gcgagtccag ctcgaagaac aggaacttgc gctccacgcg
      661 ggccaggttg ccgacgagag ccatgttggt caacaggatg cccagcaggc cgaccagcac
      721 gaagttggag tcgatcaggc tgaggaaagg gccgtcgagc aggaagaaat cggagatggc
      781 catggcgaac atattgaaca cgctggaacc cagcaggttg cccacggcca ggtcgaacgc
      841 acccagtcgc acggcggcca gcgcggcgac cagctccggc agcgaggtga cgaagctcag
      901 cagcgccgtg cccacgaagc cggtgcccag gccggtggct tccgcaatgc cgaccgacga
      961 gctgaccagt tgcggcacgg cggcgatcag gatggccccg gcgacgatga agccgatgat
     1021 gcctctgcgc agagacggga aaccctcggc cggcgccatc acggccggcg gtggcccggc
     1081 ggtggcctga ttctcgcgct gcaccaccca gatgccggcg aagtagagcg caatgagcag
     1141 caggctatcc acccctaccc agccgatggc caggtcgatg tcggcgacca tggccaacac
     1201 cgccaccagc atcaggatag tcgccagagc cgccgtcagc gaatgactga tagccacccg
     1261 ccgcagcagc ggcgcttgtc ggtgagtcag atcgacgatg gccagcaggg cgatattggt
     1321 catgttgctg ccgaagaagt tgcccgcggc caggttgagt gcgccggtgc ggaaggcgct
     1381 gatcgacgcg gtgagctccg gcagcgaggt cgccccggcc aggaagatgg tgccgacgaa
     1441 caacccgccc agcccggtgc gcactgcgat gacgtcgcta tattcagcca gcttgatggc
     1501 cgccagcacg acaacggccg aactggccac gaatagtatc cagaccatcc tctagcgctc
     1561 cctgccttct ttggctgtgc tcgggttgcc gggcagaccc cacacccgcg aaacgggcag
     1621 ggcgaacata atccccgtgt tggggccgtc gagcttgccg acgatgcgct cggtggcggc
     1681 gacggccgcc tcgatgacgg ccatgtcgtc gacgaccgcg aacagggtgt tgtggccggc
     1741 ctcggtgttg ccgaagatgc ggccgaagcc ggaataggcc acgtctggtc gatggcgcag
     1801 caggacacga tgcagtccgg tcgattcgag gatagtcacc ccgcggatgc cggcgcgctc
     1861 ccagcttgcc agcacctctt cccggtgtgc gacattatcc agcaccatga tcagcatgta
     1921 catatttgtt acctcccatc cccccacaac catgtctgtg ttcattcgcc aaagttcaac
     1981 taacgataga aatcaatcca ttgcatcgaa aacgctgaat actttccctg aaaaaagtgg
     2041 gccggcaggg tgatatcgaa cccgtcatcc gtccggctga tgtcgggaat atagattgaa
     2101 tcaggatcgg gaatctcctt caccttttca ttctcatcgg cgacatggag agcaggcggc
     2161 ggatcgggcg aattgttgct cgacaggccg gtggcgatgg tgtaggggcc gtaatggatc
     2221 tccagccgct ccagtcccct gagccgcaga tggatcgtga tctgctccgg ccattcgcca
     2281 tcgatcagcg tcgccgtcag gccgttgatg ccgtgggcgt cggtcatgtc gatgagagca
     2341 tggccgtcac tgaatgtgag cgcggccgaa ctggcttcgg gccggtcgag ggtgatgtcg
     2401 atcatgggtt ggcgtgcctc cgcgctgcag gtcgcgagca tgaacaccag tatcatgctt
     2461 gcggcaagtt gtgatttgcg ggtggtggtc atgctcatta atgatcattc ggaaccgatt
     2521 ggccggttca actccatctt cttcaatcga tacaacatct cctgcgtcaa ctgtcggtgc
     2581 ttgccgctga tgtcggcctg gatgccgaac aacacgatca gcgtgccgac cagcagcagc
     2641 cccgtgccca gcgtcagcga ttgcacgaag cgccccacgc cgctctcccc ggtcaggtag
     2701 ttgaacatga accgcgccca gagagctatt ccggccagca ggaacggcgc ggcgatatag
     2761 ctgaaggtgc gcagcggttc atagaaggca tagaggcgca ggatcgtccc cgcctgggcc
     2821 ttgatgaaat gccacatgct gcgctgcagg cgcgacggcc gcgtgggcgg gttggtcgtc
     2881 accggcacgc tgacgatccc cagcccgctc ttgcccgcct ggatgatcgt ctccagcgtg
     2941 tagctgtagc gggtcaggat ggtgaagcgc agcgccgcct cacgcgagta ggcgcggaag
     3001 ccgctgacgg ggtcggccac gtccacgccg ctgacgttgc gcaccatcca gctgcccaga
     3061 gccagcatcc gcttcttcat gggggagaag tgagggatgg tctgcacctg ccggtcgccg
     3121 ataaccatgt cggcctgtcc ggccagcacc ggccccacca ggtcggggat gtagcggccg
     3181 ggatactggt tgtcggcgtc ggtgttgacg atgatatccg cccccagccg caggcaggcg
     3241 tcgatgcccg tctggaacgc ctgggccagc cccaggttgc gctggtggcg gatgatatgg
     3301 ttgacgccca gcgcttcggc gacggccgcc gtgccgtcgt cgctgccgtc atcgatgatg
     3361 agcgtttcga tgcagtcgat gccgggaatg gccgcgggca gctcgaccag cgccgccggc
     3421 agcgtttccg cctcgttata gcagggaatc tggatgatga gtttcatgaa ttatgccaag
     3481 ggttagcttg ccacttgctc tcgcaatctc aatcgcgtcc ggcgatgatg tcgtcaagtt
     3541 cgaccaggtg cagcccttgc cagagataga cgcgcaggtc gacgcgcaga tcgtcgctga
     3601 cgaccacccc ctcggcctgc agcagctcct tttggcgact tgcgccgccg tccatgtcgg
     3661 gcgtactgat gcggccggcg tggttgatga cgcgctgcca cgggacgggc ggctcgttgt
     3721 cgcgcttgct gcccagcgcg ctcagcgcat agcccacggc gcgggcggcg ttcgggcggc
     3781 ccagcatcgc ggcgacgcgg ccgtagctgg tgacgcggcc gcgcgggatg cgccgcacga
     3841 cggcatagac ctgtcgatag aaatccggcc cttttgtgct catgatgatg ggtgtagtgt
     3901 agcgaaatcg gagagggcgg ccaaatggga gaggttgtag cgcaatctgt cagattgccg
     3961 tccggatgcg cggcccgcaa gctgacagct tgcgctacag ctcgcgtaaa tggtggtttg
     4021 caacggtgcg gtgtactaaa ctacccaact gatgactaaa ttcgataacc ttgatctgat
     4081 cgtccgcgac gtgggcgccg ccgtggactt tttcgagcgc gtcgtaggat tggaagcgcg
     4141 ggtcgtcgat gaacatttcg ccgagctgga atcgggcgaa gtgactatca tgctcagccc
     4201 gacggcgatg atccccgtca agcctgcatc ggggatgatc cttcatttcc gggtggagaa
     4261 cgtccaaaag gcgctggaaa gagccaatgc ctatggcgcg actgttctca tggagccgat
     4321 gctgaccgat tggggcaccg agtcgctgct catcgccggg ccggaagaga tagtgatcga
//
LOCUS       DAOMQG010000296         4378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8331_length_4378_cov_5.847097, whole genome shotgun sequence.
ACCESSION   DAOMQG010000296 DAOMQG010000000
VERSION     DAOMQG010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_4378_cov_5.847097"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLC06_13305"
     CDS             complement(<1..1192)
                     /locus_tag="PLC06_13305"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRO91469.1"
                     /translation="MGKIRSAFRFLFRFATIWLVDAISLSLTAWFLPGIGLGAYAGLP
                     AWLVALGAALLLGLVNLAIRPLILLAAMRQGFIILLVVGFFVNAVMLRLTAAVMGGGL
                     TVDGWLPAIVGGIVLAIVNMIVAGLVGIDDQGSFYRAVIESQLAQGQGDTPPDTYRGV
                     IVLQLDGLSYYHLQDALTRGYLPSIARMVEKDGYVVSRTDCGLSSQTSACQAGILYGD
                     NYDIPAFRWYDKAENRLYVAGNDAALINERYGHGKGLLRDGVSINNLLDGDARLSLLT
                     ATDLSGGEDEERQARANDIYLLLLNPHFFLRVVGKFIGEAMLEVWQYTLDVVRGVEPR
                     LNRLSGFYPLYRAATTVFMREVSGFLTLVQIGRGAPIIYTTWPGYDKVAHYSGPWSRH
                     AFGTL"
     gene            1379..2224
                     /locus_tag="PLC06_13310"
     CDS             1379..2224
                     /locus_tag="PLC06_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HRO91470.1"
                     /translation="MSQSLLPPLALGLVLSLLIAFLAWRRDSLSQSGAVGALVVGTLI
                     FGLGGWVWGVLLAVFFISSSLLSHFKEREKAAVAEKFEKGHRRDMGQVLANGGLGAVI
                     ALLSVVVPESAVPAGTWFFLFLGVMATVTADTWATELGTLSKGAPRLITNGRAVEVGT
                     SGGVSPLGTGVSFAGGLLIGLTAGLLAPVAGLLPWSAALPLALIGALSGAAGSLIDSL
                     MGATIQQIYFCDTCQKETERSLHRCGTTTRPLRGWAWMHNDMVNLISSLGGGLVAVGL
                     STALL"
     gene            complement(2263..4284)
                     /locus_tag="PLC06_13315"
     CDS             complement(2263..4284)
                     /locus_tag="PLC06_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91471.1"
                     /translation="MTEAIISPTRGSRLRAVLHSRNRLLWVSVALVLAGLLYLSTFQT
                     HINGSDHPFTTDVGEIQNALPRWGIIHHSSYPLYSATGSLFVTVLRLVGIPPAAGASL
                     LSLAWGLITIALLVVFTMELGATGPWAALGALAAAVSVSVWMDSSLAELHTMTTALTM
                     ATLLFALRFGRSGARSDLLWLVFFFSQGVFHQRSIILMTPPVLLLIWPHLLTPFHMGR
                     RALLLVVGVALLAPLTYLYLPLRVWTGADWVFGSPGTWDGFWKLFFDNRAARVFELDA
                     DWGLRLRTARDILNDDLWLPLLAVGLLGLWLVPEKPAISQSVSGGLNQPSVVERQEFL
                     GRWVTLRIKLALTTVWALNLLLTLLIWEDRVSDAQLAAKLPVLLMAGVGLGLLLEWLW
                     RRRPVAGAAAAVMLGLLLVYWAWANRPFVLSITRDNSRQAIVETLERVQPALDGRPIV
                     VQTPWGADYWALTYEQAYGGRLSGLNLVDHNARPQDVMLRGDRLVVPDQTFHIFPPDY
                     YEERLGPLYLSSAAPGVIEIGNVPLYDEATLAADPGIVPMDFDLENGARIRGTAVEWL
                     GDHAILLTIYWEADRAIEADYSIAVHLVAHDPPTGPDDVLNQADHAHPVDGWYPTTRW
                     RPGEIVRDSYLLEVPEGSHPAAIRIAMYRNDPTAGFVNTPWLSLPIPQR"
BASE COUNT          971 a         1377 c         1296 g          734 t
ORIGIN      
        1 gcagcgtgcc gaaggcgtgg cgcgaccacg gcccggagta atgggccacc ttgtcgtagc
       61 ccggccaggt agtgtagata atcggcgcac cgcggccgat ctgcaccagc gtcaaaaagc
      121 ccgacacctc gcgcatgaac accgtggtgg cggcgcgata gagcgggtag aagccggaca
      181 aacgattgag tcgcggctcg acaccgcgca ctacatccag cgtgtattgc caaacctcca
      241 gcatcgcctc gccgatgaat ttgccgacga cgcgcaggaa aaagtggggg ttcagcaaga
      301 gcaggtagat gtcgttggcg cgggcctggc gctcctcatc ttcgccgccg ctcaggtcgg
      361 tcgccgtcag cagcgagagc cgggcgtcgc cgtcgagcag gttgttgata ctcacaccgt
      421 cgcgcagcag ccccttgccg tggccatagc gttcgttgat gagcgctgcg tcgttcccgg
      481 ccacatagag gcggttctcg gccttgtcgt accagcggaa ggcggggatg tcgtaattgt
      541 cgccatagag gatgcccgcc tggcaagccg acgtctgcga cgacagcccg caatcggttc
      601 gggagaccac atagccgtct ttttcaacca ttcgcgcgat actgggcaaa tagccgcggg
      661 tcaacgcatc ttgcagatgg taatagctca gcccgtcaag ttgcaggacg atcacgccgc
      721 gataggtgtc cggcggcgtg tctccctgtc cctgggccag ttggctttcg atgacggcgc
      781 gatagaacga gccttgatca tcaatgccga caagcccggc gacgatcatg ttgactatcg
      841 ccagcacgat gccgccgaca atggccggca gccagccgtc gaccgtcagc ccaccgccca
      901 tcaccgccgc cgtcagccgc agcatcacgg cgttgacgaa aaacccgacg accaacagga
      961 tgatgaaacc ctggcgcatg gctgccaaca aaataagcgg ccgaatggcc aggttcacca
     1021 aacccagcag cagcgccgcg cccaacgcga ccagccacgc cggaagcccc gcataagcac
     1081 ccagcccgat ccccggcagg aaccaagccg tcaggctgag cgagatggcg tcaaccaacc
     1141 agatagtggc gaaccggaac aggaagcgaa aggcggagcg tattttcccc ataagtgtcc
     1201 tgcgtccaca gtaccaatat gcatgaccac acgtgatgtg ttttccgatt atgacaaaca
     1261 atcgctgaac caccaaaata tgccgggaca atcgcacata gactcactct cgcttgtagt
     1321 gcaagccgcg ggcttgcggc ttgcgccaca cgcccaaatc cggctacact acgattccat
     1381 gagccaatcc ctgttgcccc ccctcgccct cggccttgtc ctcagcctcc tcatcgcctt
     1441 tctcgcctgg cggcgcgact ccctgtcgca atcgggcgcg gtcggggcgt tagtcgtcgg
     1501 cacactcatc ttcgggctgg gcggctgggt gtggggcgtt ctgctggcgg tgttcttcat
     1561 cagctccagc ctgctctccc acttcaagga acgggaaaaa gcggccgtgg ctgaaaagtt
     1621 cgagaagggc caccggcgcg acatggggca ggtgctggcc aacggcggtc tgggggcggt
     1681 catcgctctc ctcagcgtcg tcgtgccgga gagcgccgtc ccggccggca cgtggttttt
     1741 tctgttcctg ggggtcatgg cgaccgtgac ggccgacacc tgggccacag agttgggcac
     1801 gctgagcaag ggcgcgccgc ggctcatcac caacggccgc gcggtcgagg tcggcacgtc
     1861 gggcggcgtg tcgccgctgg gcacgggcgt gtccttcgcc ggcgggctgc tcatcgggct
     1921 gacggccggg ctgctcgcgc ccgtggccgg gttgctgccg tggagcgccg ccttgcctct
     1981 ggctctcatc ggcgcgctca gcggcgcggc cgggtcgctc atcgacagtc tgatgggtgc
     2041 aaccatccag cagatctact tctgcgacac ctgccaaaaa gaaacggagc gcagcctcca
     2101 ccgctgcggc acaacaacgc ggccgctgcg cggctgggcg tggatgcata acgacatggt
     2161 caacctgatt tcgtcgctgg gcggggggct ggtggcggtg gggttgtcga ctgcgctatt
     2221 gtagagtgcc ttgcagcaca catagacgca tagacacccg ccttatcgtt gtgggatggg
     2281 taacgacaac cagggcgtgt tgacgaatcc ggctgtcggg tcgttacgat acatggcaat
     2341 acggatggcc gccgggtggc tcccttccgg cacctccaat agataactat cgcgaacgat
     2401 ctctcctggc cgccaacggg tggtcggata ccaaccgtct accggatgtg cgtggtccgc
     2461 ctgattcagc acatcatccg ggccggtcgg cggatcatgg gccaccagat ggacagcaat
     2521 actatagtcg gcctcaatgg cgcggtcggc ctcccaataa attgtcagca ggatcgcatg
     2581 atcgcccaac cattcaacgg ccgttccccg aatgcgcgcg ccgttctcca gatcgaaatc
     2641 cattgggaca atgccgggat ctgccgccag agtcgcctcg tcatagagag gcacattgcc
     2701 tatttcgatg acgcccggtg cggccgaact caggtagagc ggaccaagcc gctcctcgta
     2761 ataatcgggc ggaaagatat gaaatgtctg atcgggcaca accagccgat cgccacggag
     2821 cataacgtcc tgtggccggg cgttgtgatc aaccagattc aagcccgaca aacgaccgcc
     2881 atacgcttgc tcataagtca gtgcccaata atctgcgccc caaggagtct gaacgacgat
     2941 cggccgcccg tcgagagcag gctggacgcg ctccaacgtc tcgacaattg cctgccgcga
     3001 attgtcacgg gtgatgctga ggacgaaggg tcggttagcc caagcccaat agacgagcag
     3061 taaccccagc atcaccgcag ctgctgcgcc ggccacaggt cgccgccgcc atagccattc
     3121 aagcagtaaa cccagaccca ccccggccat gagcaatacc ggcagtttcg ccgccagctg
     3181 agcgtcgctc acccgatctt cccagataag caaagtcaaa agcaagttga gcgcccagac
     3241 ggtggttagc gccagtttga tgcgtaaggt tacccatcgg ccaaggaact cttgcctctc
     3301 gaccacagac ggctgattca acccaccgga cactgattga ctgattgccg gtttctcggg
     3361 cacaagccat aatcccaaca aacccaccgc caataacggc agccatagat catcgttgag
     3421 aatatcgcgt gccgtacgca gccgtagccc ccagtcggca tccaactcga agacacgtgc
     3481 ggcgcgatta tcaaagaaca gcttccagaa accgtcccag gtgcccggtg agccgaatac
     3541 ccaatcggct cccgtccaca cacgcagcgg caggtaaagg taggttaatg gagccagtaa
     3601 agccacgcca acaacgagca gcagtgcccg tcgccccatg tgaaacggag tcaacagatg
     3661 gggccagatg agcagaagca ctggcggcgt cattagaatg atggagcgtt ggtggaaaac
     3721 accctggctg aaaaagaaaa ccagccacaa caagtcggat cgcgcacctg aacggccgaa
     3781 acgcaaggca aacagcagtg tcgccatcgt cagggcggtg gtcatcgtat gcaattcggc
     3841 caacgaggaa tccatccaaa cagagacaga gacagccgcc gccaacgcgc cgagggcggc
     3901 ccatggcccg gtagcgccca actccatggt gaacacgact aacaaggcga tcgtaatcaa
     3961 tccccaggcg agtgaaagaa gcgacgcccc cgctgccgga gggatgccca ccagacgcag
     4021 caccgtcacg aacagcgaac cggttgccga ataaagcggg tagctgctat ggtgaatgat
     4081 accccagcgc ggcaaagcat tctgaatctc acccacgtca gtcgtaaatg gatgatcgct
     4141 gccgttgata tgagtctgaa aggtagacaa gtagagcaat ccggccagca ccagagcaac
     4201 agaaacccaa aggaggcgat tccgggaatg caatacggcc cgcaaacggc tgccgcgggt
     4261 gggcgatatg atggcttcag tcataatcag gccgaataat gaggtgaaat ccaacggtag
     4321 agatcgatca ctatctcttc cggcccggcg atgagcagcg actcggtgcc ccaatcgg
//
LOCUS       DAOMQG010000297         4371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8350_length_4371_cov_6.059082, whole genome shotgun sequence.
ACCESSION   DAOMQG010000297 DAOMQG010000000
VERSION     DAOMQG010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8350_length_4371_cov_6.059082"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..2048)
                     /locus_tag="PLC06_13320"
     CDS             complement(21..2048)
                     /locus_tag="PLC06_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91472.1"
                     /translation="MRARLVAVLVVLIILTTLSAGVPAYLLARRQLEQGAWERVDATR
                     RATESLYDAALGRAVDLTTLLGERPTLRRLIADSDTAALETYLAAFREQSDLDLIQVC
                     SGARVIVAIGETGNLCAIGPGAGVELVDERPVMLARVAIPGDGQPQNSDKVVAGVRLD
                     AEFLLQLSANTGMEQSIIGPDDHALISTLPPGPTTVELLPDGRLLTVQETGGGESPFF
                     AAQFLLPGHASDLYAEVALPVAGLRATERDALAILVAGSVAVAAAGAVVGAWFVRRLT
                     APLESLTHAAEQISGGDLTAPIPRVAAPPEMATLAAALERSQGTMLAALAERSQSRDW
                     LNTLVQSVVEGVITFDTRGRVTFMSQGAEIMTGWTSEETIGRPINDLLPPPDPGAADT
                     FLDHLPPAGEKREIETRTRSGRSLVLAATGARMVPPSGDTVQVALVLRDVTEEQALRN
                     LRSFFLANISHEFRTPLSTLGASLELLMEQADDLTAEEIRELLRPTHMSLLSLQTLID
                     NLLESSRIEAGMFALRRQPVAMEELILDALAIARPLLDRRGQSVSITEPAALEPVTVD
                     RGRMTQAMVNLLSNASKYSPIGRPIDLWVEQRAGAVRVGVADRGPGIPPEQRDNVYRR
                     FVRLDEVGQEQQGMGLGLYLVKKIVEAHDGTVGVDDRAGGGAVFWLELPGA"
     gene            complement(2177..4201)
                     /locus_tag="PLC06_13325"
     CDS             complement(2177..4201)
                     /locus_tag="PLC06_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRO91473.1"
                     /translation="MDQDFSLFVTITVAVMLAFAGGFVARKLKLPSIVGYLVAGLVLS
                     PFTPGIDGDTQAAAQLADMGIIFIMFGVGLHFSLRDLWSARTIVLPGALLQITLTTVA
                     GTAVGLLWGWPLGSAVILGLSISVASTVVLLRNLGDNGLVNTIHGRTAIGWLVVEDLF
                     TIAMLVLLPLLVNQSGGNPIISLGWALLKAGLFVAIMVVLGARLMSSVLTRIAHTRSR
                     ELFILAVVALALGTAYLANLFFGVSFALGAFLAGVVVGESDVRHQVGAEIVPFRDFFS
                     VLFFVSVGMLIDPTVVVANLGKVFMLAALVIIGKPLFALLLSFLLPASARTMIVVAAA
                     LSQVGEFTFLIGQTGVRLGLLANDQYGLILAAAVLAIIANTFVFRLIPSVERTLRRAP
                     ALWRRMDHGGPVVEPLHQELENHVVIVGYGRVGRHIGSILQRLGVEFLVIEQDLAYAS
                     ELQSQGIRTLFGDAANSEILNHVCLDCAEALVVTVPNETAAELIVGAAHDISPDLVII
                     ARSETLDRLDHLVSLGATHIINPEMEGGLEILRYTLLTLGYSSDQIQNYIDIVRSDTY
                     TGILPGDRGYPVLDQLLMSTRDVEIAWHALGEGSPLVDRTLAEADLRSRVGASVVALV
                     RNRQVMANPKSNIRFQTGDVLGLIGDVDELEAAARLIAPEAVVAQPAFKT"
BASE COUNT          795 a         1446 c         1491 g          639 t
ORIGIN      
        1 attcgtaatt cgtaattcgt tcacgccccc ggcagttcca gccaaaacac cgccccgccg
       61 cctgcccggt catcgacccc caccgtgccg tcatgggcct cgacgatctt cttgaccaga
      121 taaagcccca gccccatccc ctgctgctcc tggcccacct catcgagccg cacgaagcgc
      181 cggtagacgt tgtcgcgctg ctccggcggg atacccggcc cgcggtcggc cacgcccacg
      241 cgcaccgccc cggcccgctg ctccacccac aggtcgatcg gccggccgat ggggctgtat
      301 ttcgacgcgt tcgagagcag attgaccatc gcctgcgtca tccggccgcg gtcgacggtc
      361 accggctcca gcgccgccgg ctcggtgatc gagaccgact gcccgcggcg gtcgagcagc
      421 ggccgggcga tggccagcgc gtcgaggatc agctcctcca tcgccaccgg ctgccgccgc
      481 agcgcgaaca tccccgcctc gatgcggctg ctctccagca ggttatcgat cagcgtttgc
      541 aggctcagca ggctcatgtg cgtcggccgc agcagctcgc gtatctcctc ggccgtcagg
      601 tcgtcggcct gctccatcag cagctccagc gacgcgccca gcgtgctcag cggcgtgcgg
      661 aactcatggg agatattggc caggaagaaa gagcgcagat tgcgcagcgc ctgctcctcg
      721 gtcacatcgc gaagcaccag cgccacctgc accgtgtcgc ccgacggcgg caccatgcgc
      781 gcgcctgtgg ccgccagcac caggctccgg ccggagcgcg tgcgcgtctc gatctcccgc
      841 ttctcgcccg cgggcggcag gtggtcgagg aaggtgtcgg ccgcgccggg gtcgggcggg
      901 ggcagcaggt cgttgatcgg ccggccaatg gtctcctcgc tcgtccagcc ggtcatgatc
      961 tccgcgccct ggctcatgaa ggtgacgcgg ccgcgagtgt cgaaggtgat gaccccctcg
     1021 acgaccgatt gcaccagcgt gttgagccag tcgcgggatt gcgagcgctc ggcgagcgcg
     1081 gccagcatcg tcccctggct gcgttccagc gcggccgcga gcgtagccat ctccggcggc
     1141 gcggcaacgc gcgggatggg cgcggtcaga tcgccgccgc tgatctgctc ggcggcatgg
     1201 gtcagcgact ccagcggcgc ggtgaggcgg cggacgaacc acgccccgac caccgccccg
     1261 gcggcggcca cggccacgct gccggccacg aggatggcca gcgcgtcgcg ctcggtggcc
     1321 cgcagccccg ccacgggcaa agcgacctcg gcgtagaggt cggacgcatg gccgggcagc
     1381 aggaattgcg cggcaaagaa gggggactcg ccgcctccgg tctcctgaac ggtaagcagg
     1441 cggccgtcgg gcaatagttc aacggtggtt gggccgggcg gcagagtcga gatgagggcg
     1501 tggtcgtcgg ggccgatgat cgactgctcc atgccggtgt tggcgctcaa ttgcaggagg
     1561 aactcggcat cgaggcgaac gccggcaacg actttgtcgc tgttttgcgg ctgcccgtcg
     1621 ccggggatgg cgacacgggc aagcatcacc ggccgctcat ccaccagttc gacgcccgcg
     1681 ccggggccga ttgcacagag attgccggtc tcgccgatgg cgacgatgac ccgcgccccg
     1741 ctgcacacct ggatgaggtc cagatcgctt tgctcgcgga aggcggccag ataggtctcc
     1801 agcgcggccg tgtcgctgtc ggcgatgagg cgacgcagag tcggccgttc gcccagcagc
     1861 gtggtcaggt caacagcgcg gccgagcgcg gcgtcataga gcgactcggt ggcgcggcgg
     1921 gtggcatcga cccgctccca cgctccctgc tccagttggc ggcgggccag caaataagcg
     1981 gggacgcccg cgctgagggt agtcagaatg atgagaacga caaggacggc gacaaggcgg
     2041 gcgcgcatgg aaattctgga aaccttgcct ctttcggcaa agcctcaacc tggacaggtt
     2101 gagctacagc ggcaaacctg acaggtgggc gacgacaaat ctcactgtta tcagagtgcc
     2161 cctgatcgcc cacctgtcag gttttgaagg ctggttgagc tacaactgcc tccggcgcga
     2221 tgagccgcgc ggccgcttcc agctcgtcga cgtcgccgat gagacccagc acgtcgcccg
     2281 tctggaagcg aatgttggac ttggggttgg ccatcacctg ccggttgcgc accagcgcca
     2341 ccactgacgc ccccacccgc gagcgcaaat ccgcctcggc cagcgtgcgg tcgaccagcg
     2401 ggctgccctc gcccagcgca tgccaggcga tctccacgtc gcgcgtcgac atgagcagct
     2461 gatccagcac cggatagccg cggtcgccgg gcaggatgcc cgtgtaggtg tcgctgcgca
     2521 cgatgtcgat gtagttctga atctggtcgg acgaatagcc cagcgtcagc agcgtgtagc
     2581 gcagaatctc cagcccgcct tccatctccg ggttgatgat gtgggtggcg ccgaggctga
     2641 ccagatggtc gagccggtcg agcgtctccg agcgggcgat gatgaccagg tcgggtgaga
     2701 tgtcgtgggc ggcgccgacg atcagttcgg ccgccgtctc attgggcacg gtgacgacga
     2761 gcgcctcggc gcaatcgagg cagacgtgat tgaggatctc ggagttggcc gcgtcgccga
     2821 acagggtgcg gatgccctgg ctttgcagct cggaggcgta ggccaggtcc tgctcgatga
     2881 ccagaaactc cacccccagc cgctgcagga tggagccgat atggcggccg acgcggccgt
     2941 agccgacgat gaccacgtgg ttctccagct cctggtgcag cggctcgacc accgggccgc
     3001 cgtgatccat gcgccgccac agggccgggg cgcgacggag cgtccgttcg acggacggga
     3061 tgagccggaa gacgaaggtg ttggcgatga tggccaggac ggccgcggcc aggatcagcc
     3121 cgtattgatc gttggccagc aagcccagcc ggacgcccgt ctggccgatg aggaaggtga
     3181 actcccccac ctggctaagc gcggcggcga cgacgatcat cgtccgcgcg gaggcgggca
     3241 gcaggaagct gagcagcagc gcgaagagcg gcttgccgat gatgaccagc gcggccagca
     3301 tgaacacctt gcccagattg gcgacaacca ccgtcgggtc gatcaacata ccgacggaga
     3361 cgaagaacag gacagagaag aagtcgcgga acgggacgat ctccgccccc acctggtggc
     3421 ggacgtcgga ctcgccgacg accaccccgg ccagaaacgc gcccagcgcg aacgacacac
     3481 cgaagaaaag gttggccaaa taggccgtgc ccagcgccag ggcaacgacg gccaggatga
     3541 acaattcgcg cgagcgggtg tgggcgatgc gggtgagcac cgacgacatg agccgcgccc
     3601 ccaggacgac catgatcgcc acaaagagac cggctttcag cagcgcccag ccgaggctga
     3661 tgatcggatt gccgccggac tggttcacca gcagcggcaa gaggacgagc atggcgatgg
     3721 tgaacaaatc ttcgacgacg agccagccga tggcggtgcg gccgtggatg gtgttgacca
     3781 ggccgttgtc gcccaggttg cgcagcaata cgacggtgct ggcgaccgaa atcgacaggc
     3841 cgaggatgac cgccgacccc agtggccagc cccacagcag gccgacggcc gtcccggcca
     3901 ccgtggtgag ggtgatttgg aggagagcgc cggggaggac gatggttcgc gcgctccaga
     3961 ggtcgcgcag ggaaaagtgc agccccacgc cgaacatgat gaagatgatg cccatgtcgg
     4021 ccagttgcgc ggccgcctgg gtgtcgccgt cgatgccggg ggtgaacgga ctcagcacca
     4081 gcccggcgac gagatagccg acgattgacg gcagcttcag cttgcgagcg acgaagccgc
     4141 cggcaaaggc cagcatgacg gccaccgtga tggtgacaaa gagcgaaaag tcctgatcca
     4201 tggaggaagt atagcggaat taaaccgaat gaccgatcat attttgaaag taaaccggcc
     4261 tcctttggcc gttccgccgc cgtccttaat gaaatgaacg cccagaccct atcgcgcggg
     4321 gtcgaacacg atcacctcat aggaaaagct gcgcgcctgg ccctcgatga g
//
LOCUS       DAOMQG010000298         4336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8431_length_4336_cov_8.430507, whole genome shotgun sequence.
ACCESSION   DAOMQG010000298 DAOMQG010000000
VERSION     DAOMQG010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8431_length_4336_cov_8.430507"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="PLC06_13330"
     CDS             <1..1560
                     /locus_tag="PLC06_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO91474.1"
                     /translation="LGLLAMRSVDKLFFLPQTGLLAHALGWGDGLPTNEIAGVLTLFV
                     PFVVALVYACLITGRRQQFLRLLPVALLMVGALVLTQSRTGLTATVVGTLLALSAAGS
                     MNRKWLIVVAVLVGMGALLAWLTPARDWFVFAGANSWSSVVGPRLGIWNQAIDAIRDH
                     PLWGMGIGLFGSIARFIYPLITPEAGPVLEDAHNLYLQTALDFGVAGLFVFLVIGGLV
                     FISAIRLTRARPPHTLSRLWAAGLLGGLVAHALYSLTDAVSLGTLAGVPLWFAFGLVM
                     GASRGRLQLGWPNPARLAFGGALLVILAISALALPVNRAGQLAVHTLLDPAADRVVVA
                     GSFEPLVSRNCRAGWYEGLVRHWAGDAAGRAAAWGDLVGCSANYTGYMAVLAPKDIEL
                     ARKAIAAQPDDAAGYFWLAPALAADAPDEAIALYRQGLELSPADGHRWLALAELLEPR
                     DPAAALEAYLQACRHGDPGANGCLRAGGLAEAQGDVTAAIDYYRLSNWSSALERANEL
                     EQQLIVGQSQE"
     gene            1634..3709
                     /locus_tag="PLC06_13335"
     CDS             1634..3709
                     /locus_tag="PLC06_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRO91475.1"
                     /translation="MAILTTSNVGQSFGDYDVFSGVSVSIPRDGKVGLVGPNGVGKTT
                     LLLILAGKAAPSSGLIHVARGTRIGYLSQAAADAFIDRTAEHTVHEEMRAVFAPLQAM
                     EARLREMEAEMAGDAASEALLDEYSRLQVQYELDGGYDYETRIRMVLTGLGFRPDVWD
                     RPLSQLSGGQKTRMLLGRLLLESPDLLILDEPTNHLDVQAIEWLESMLAGWPGAVLVV
                     SHDRYFLDRVATTIWEMSAAGVETYRGNYSAYLTQRQDRWERRQREYDAFLERVAKEM
                     DFIRRNIAGQRTQMAWGKLSRLSREVEAVRVGGLGAIGDLSSKGWSRIASELDLRRPA
                     ATLAELQEAIAALPAPARPPVVNMRLNTAWRSGNIVLRAADLIVGYPGYPLFTVDQLM
                     LHRLETAALIGPNGTGKTTFLKIILGQLEPLAGELKLGASLKVGYFAQTQEALDPTAT
                     VIDELLRHKDMPISEARNYLARYLFRGDEVFSQVGVLSGGERARLALAILVLEEANFL
                     LLDEPTNHLDIASQEVLQSALEAFDGTILLVTHDRYLVDRLASQIWELRRDDDGLRME
                     VFDGPYREYLAAKTAKPVVEAAPHVGHGTNGIDGTNGTAAPARMSKNEQRRLAEAVAA
                     LEAEIGATEERLATLSEAMQVAAEAMDYAELMRLTADYEAAGEHLAKLFEDWEQMPHE
                     PAGDSRTDG"
     gene            3675..4262
                     /gene="coaE"
                     /locus_tag="PLC06_13340"
     CDS             3675..4262
                     /gene="coaE"
                     /locus_tag="PLC06_13340"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871210.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRO91476.1"
                     /translation="MSRRVILGLTGNIATGKSAVMRLAAERGALTIDADKVVHELLDG
                     DATIQAAVVEAFGPEVRRADGRIDRAALGHIVFGDAGKLQQLEGLLHPAARAEIARRV
                     AAADARVVVIEAIKLLEGPLATLSDQIWVTTCTRETQMERLRVCRGMDEASAAARIDA
                     QPPQAGKIARADVVIHTDGLMADTQAQFEAAWEWD"
BASE COUNT          698 a         1308 c         1536 g          794 t
ORIGIN      
        1 ctcggcctgc tggccatgcg cagtgtggac aagctgttct ttttgcccca gacgggtctt
       61 ctggcccatg ctctggggtg gggcgacggc ctgccgacga acgaaattgc cggcgtattg
      121 accctgttcg tcccgtttgt cgtcgctctg gtctatgcct gtctgatcac cgggcggcga
      181 cagcagtttc ttcggctttt gccggtggcg cttttaatgg tcggcgcgct ggtgctgacc
      241 caatcgcgca cggggttgac ggcgacggtc gtcggcacgc tgttggcgct ttcggccgcc
      301 ggctccatga accgcaagtg gctgatcgtc gtggccgtgc ttgtcggaat gggggcgctg
      361 ctggcgtggc tgacgcccgc gcgggactgg tttgtcttcg ccggggccaa ctcctggagc
      421 agcgtcgtgg ggccacggct gggaatctgg aatcaggcga ttgacgctat tcgcgatcac
      481 ccgctatggg gcatgggcat cgggttgttc ggctccatag cccgcttcat ctacccgctg
      541 attacgccgg aggccgggcc tgtgctggaa gacgcccata acctctattt acaaacggcg
      601 ctggatttcg gcgtcgcggg tttgttcgtc tttctggtta tcggggggct ggttttcatt
      661 tccgccatcc ggctgacccg cgcccgcccg ccgcacacat tgtcgcggct ctgggcggcc
      721 gggctgttgg gcgggctggt ggcccacgcc ctctatagtc tgactgacgc cgtgtcgttg
      781 gggacattgg ccggtgtgcc gctgtggttt gcctttggtc tggtgatggg cgcgtcgcgc
      841 ggccgtttgc agcttggctg gcccaacccg gcgcgcctgg cgtttggcgg ggcgttgctg
      901 gtgatattgg ccatctccgc cctggccctg cctgtgaatc gcgccggaca actggcggtt
      961 cataccttgc tcgatccggc ggccgaccgg gttgtcgtgg ccggatcgtt cgaaccgctg
     1021 gtgagccgga actgtcgtgc cggttggtat gaggggctgg tgcgccactg ggcgggagac
     1081 gcggccgggc gcgccgctgc ctggggagat ctggttggct gctctgccaa ctacaccgga
     1141 tacatggccg tcctggcccc taaagatatt gaactggcgc gaaaggcgat cgccgctcag
     1201 ccggatgatg ccgcgggcta cttctggctg gctcccgcgc tggcggccga cgcgcccgac
     1261 gaagccatcg cgctctatcg ccaagggctg gagctgtcgc cggccgatgg tcatcgctgg
     1321 ctggcgctgg ccgagctgct ggagccacgc gacccggcgg ccgcattgga ggcctatctg
     1381 caggcctgtc gccatggcga tcccggagcc aacggctgtc tgcgcgccgg cgggctggcc
     1441 gaggcgcagg gagatgtgac ggcggccatc gactattatc gcctctcgaa ctggagcagc
     1501 gcgctggaaa gggcgaacga gctggagcaa cagctcatcg ttgggcaatc ccaggaataa
     1561 aatcccaaac gcataaacgc gataacccgc gccatctccc cggcttcgcc aaacggcgct
     1621 ataatccggc ttcatggcga tattaacgac aagcaacgtc gggcaatcgt ttggcgatta
     1681 tgacgtcttc agcggcgtca gcgtcagcat cccccgcgat ggcaaggttg gcctggtggg
     1741 gccgaatggc gtcggcaaga cgacgctgct cctcatcctg gccggcaagg ccgccccgtc
     1801 gagcgggctg attcatgttg cccggggtac gcggatcggc tatctgtcgc aggcggcggc
     1861 cgatgccttc atcgaccgga ccgccgaaca caccgtccac gaggagatgc gcgccgtctt
     1921 cgcgccgctg caggcgatgg aggcccggct gcgcgagatg gaggccgaga tggccgggga
     1981 cgcggcttcc gaggcgctgc tggatgagta cagccggttg caggttcaat atgaactgga
     2041 tggcggctat gattacgaga cgcgcatccg catggtgctc accggtctgg gcttccggcc
     2101 cgacgtgtgg gatcggccgc tgtcacagtt gagcggcggc cagaagaccc gtatgctgct
     2161 cggccgcctg ctgctcgaat cgcccgacct gcttatcctc gacgagccaa ccaatcacct
     2221 ggacgtgcag gccatcgaat ggctggagag tatgctggcc ggctggccgg gggcagtgct
     2281 ggtcgtcagt catgaccgct actttctcga ccgcgtggct acgacgatct gggagatgag
     2341 cgcggccggc gtcgagacct atcgcggaaa ctacagcgcc tatctgacgc agcggcagga
     2401 tcgctgggag cgccggcagc gcgaatatga cgcgttcctg gagcgggtgg ccaaggaaat
     2461 ggatttcatc cgtcgcaata tcgccgggca gcgcacgcag atggcctggg gcaagctctc
     2521 gcgcctcagc cgcgaggtgg aagctgtccg cgtcggcggg ttgggagcca tcggtgactt
     2581 gagcagcaag ggctggagcc gaatcgcgag cgagctggac ttgcgccgcc cggcggccac
     2641 gctggccgag ttgcaggagg ccatcgccgc gttgcccgcg cctgcccggc cgccagtggt
     2701 caatatgcgc ctcaataccg cctggcgcag cggcaatatc gtcctgcgcg cggccgatct
     2761 gatcgtcggc tatccggggt atccgttgtt caccgtcgat caactgatgc tccatcgcct
     2821 ggagacggcc gccctcatcg gccctaacgg cacaggcaaa accacctttc tgaagatcat
     2881 cctgggccaa ctggagccgc tggccggaga gttgaagctg ggagcgagcc tgaaggtcgg
     2941 ctactttgcc cagacccagg aggcactcga tccgacggcg acggtgattg acgagctgct
     3001 gcggcacaag gatatgccta tcagcgaggc gcggaactat ctggcgcgct atctgtttcg
     3061 cggtgacgaa gtctttagcc aggtcggcgt cctgagtggc ggcgagcggg cgcggctggc
     3121 gctggccatc ctcgtgctgg aggaggccaa cttcctgctg ctcgatgagc cgaccaacca
     3181 cctcgatatc gcctcgcaag aggtgctgca gtcggcgctg gaggcgtttg acggcacgat
     3241 tctgctggtg acccatgacc ggtatctggt cgaccgattg gccagtcaga tttgggagtt
     3301 gcgccgggac gacgacgggc tgcggatgga ggtgttcgac gggccatatc gcgagtatct
     3361 ggcggcgaag acggccaagc cggtcgttga ggcagcgcct cacgttggcc atggcacgaa
     3421 tggcattgat ggcaccaatg gcacggccgc gccggcgcga atgagcaaga acgagcagcg
     3481 ccgattggcc gaggcggtgg cggcgctgga ggccgaaatc ggggcgaccg aggagcggct
     3541 ggcgacgctg agcgaggcca tgcaggtcgc cgccgaagcg atggattacg ccgagctgat
     3601 gcgcctgacg gccgactatg aggctgcggg cgaacatctc gcgaaactgt ttgaggattg
     3661 ggagcagatg ccacatgagc cggcgggtga ttctcggact gacgggtaat atcgccacgg
     3721 gcaagtcggc ggtgatgcgg ctggccgccg agcgcggagc gctgaccatt gacgccgata
     3781 aggtggtaca tgagctgctc gacggcgacg cgacgattca ggcggccgtg gttgaggcct
     3841 ttgggccgga agtgcgccgg gccgacgggc gcatcgaccg cgccgcgctg gggcacatcg
     3901 tcttcggcga cgcggggaag ctccaacagc tggaagggct gctgcatccg gcggcccgcg
     3961 cggagatcgc ccgacgggtg gccgcggccg acgcgcgggt ggtggtgatc gaggcgatca
     4021 agctgctgga agggccgctg gcgaccctta gcgaccagat atgggtgacg acctgcacgc
     4081 gggagacgca gatggaacgt ttgcgcgtgt gccgggggat ggacgaggcg tcggccgcgg
     4141 cgcgcatcga cgcccagccg ccgcaagccg ggaagatcgc ccgcgccgat gtggtcatcc
     4201 acaccgacgg cctgatggcc gacacgcagg cccaattcga ggcggcgtgg gagtgggatt
     4261 aattacgaat gggggaacgc cgctaacgtc actcatgtaa ctaacggtac taatggcatt
     4321 cttccattcg taattc
//
LOCUS       DAOMQG010000299         4308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8507_length_4308_cov_10.030802, whole genome shotgun sequence.
ACCESSION   DAOMQG010000299 DAOMQG010000000
VERSION     DAOMQG010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_4308_cov_10.030802"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLC06_13345"
     CDS             complement(<1..743)
                     /locus_tag="PLC06_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91477.1"
                     /translation="MMAAAMIHDQHHATGHGVTHEPGTPSLEMTDVWAAYDGGLSSGV
                     GPRHALEGITFRVARGERVAVVGPNGAGKSTLFKIIAGTLRPERGQVTVYGHGPAGHI
                     CIAYVPQRNQVDWNFPVTVEDVVMMGRVGQIGLLRRPGKGDWAIVRAALGRVGAADLA
                     HHRIGELSGGQQQRVFLARALAQQAELLLLDEPLNGLDVPTQEAIIGILDDLRTGGVT
                     VLLATHDLDMAAERFDRIMLLNRRIIAFES"
     gene            complement(740..1765)
                     /locus_tag="PLC06_13350"
     CDS             complement(740..1765)
                     /locus_tag="PLC06_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRO91478.1"
                     /translation="MIHIHPAQRRRLGVYHVMLALMLSGLIGCSGNRPTSNENTLEPN
                     AAGILALPDVAARTHEGERLRVLATTSIIGDVVHQVGGDAIDLTVLMRPGQDPHSYQP
                     GAADLAAAADANVIFVNGWNLEEALVKDLTTIGHQAIIVPISAGIEPRKMTAGAEADD
                     HGPFDPHVWQDVGNVMVWVDNVRQVLSAADPANEATYAANAERYRAELQQLDGELRAR
                     FDAIPSERRVLITNHESLGYLADAYGFRIAGTIIPSVSTLAEPTAAALANLISTMKAE
                     NICTIFLETTARDQLARTLEKELTGCEKVRLIPLYTGSLGPVGSDADTYIGMMRANAA
                     ALEEGLQ"
     gene            2121..>4308
                     /locus_tag="PLC06_13355"
     CDS             2121..>4308
                     /locus_tag="PLC06_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRO91479.1"
                     /translation="MIPVENIERDVEAEALAVAWQAATVDYSFVPLPDIKERFLRLAA
                     SVLGAMGAEPDPVALTAHGRSIGRELVELNLHKPKVLENTLAVLTDQLAGKNVPGQLV
                     ALISGIATGFTAALEALLLRQQENINHAATAVLKKTQEDLHFSRNHLATANRELSAQI
                     TERIHAEERQREFAERLQHLHRIDTAILSAESLEAIADIAINFISHLIPSVLISISLL
                     DFDERRLEIIGSTDPVHFPPGREIPVMLLEVLARLEKDGAAYIADFATIPNPTPVAIE
                     IAGLGGHSSLIVALRYQGWPIGTLGITLGEVRPFSEDEQTLARELAASVAVALQHRRL
                     LQAEREAHEREATLREVAAALTLDLSLDEVLNHVLAQLERVITSDSSSIMLIRGHDLE
                     IACQRGVASKYEDLKKLVKLQPRSIRLVLETARPNIINETTTSPDWIVAPGREYIRSW
                     MGVPLLVKGNCIGIMNLDRRDPHAFSARDMELAAAFANQAAVAIDNARLFAHQQRYAE
                     EMEQGVRERTRDLEVLYGITATAVGNADVTGFLQGAMGLTAGAFGCATAAAYLSVGNG
                     SGLQLAALLEGGNPSLADLLAELGEDSPLLRRPLVDGLPEILLGSDLPPQWGESAGLV
                     LATVPLRSRGQSLGVLALLCHEDAQLTSVAPDLLTTIADQIGAVVDNIRLHQIARQSA
                     IIEERERIARDIHDQVTQSIYSASLFAEAARGAAESGNMAKLQQHHQ"
BASE COUNT          798 a         1336 c         1371 g          803 t
ORIGIN      
        1 gattcaaagg cgatgatccg gcgattcagg agcatgatcc ggtcgaagcg ctcggcggcc
       61 atgtcgagat cgtgggtggc cagcaggacg gtcacgccgc cggtgcgcag gtcatcgagg
      121 atgccgatga tcgcttcctg cgtggggacg tccagcccgt tgagcggctc gtccagcaac
      181 aaaagctccg cttgctgggc cagcgcgcgg gccaggaaca cccgctgctg ctgcccgccg
      241 gacagttcgc cgatacggtg gtgggccaga tcggccgcgc cgacacggcc cagcgcagcg
      301 cgaacaatgg cccagtcacc cttgcccggc cgccgcagca gcccgatctg ccctacgcgc
      361 cccatcatca ccacgtcctc gaccgtcacc gggaagttcc agtcgacctg attacgctgc
      421 ggcacatagg cgatgcaaat atggccggcc gggccatgtc cgtagaccgt cacctggcct
      481 ctctccgggc gcagcgtccc ggcgataatc ttgaaaaggg tgctcttgcc cgcgccattg
      541 gggccgacga cggccactcg ctcaccgcgc gccacgcgga aggtgatacc ctccaacgcg
      601 tggcgcggcc cgacgccgga ggacaggcct ccgtcgtagg ccgcccacac gtcggtcatc
      661 tcaagggaag gcgtgcctgg ttcgtgggtg acgccgtggc cagttgcgtg gtgttggtcg
      721 tggatcatcg cggccgccat cattgcaacc cctcctccag cgcggccgca ttggcgcgca
      781 tcataccgat ataggtgtcc gcatcgctcc cgaccggccc caacgaaccg gtatagagcg
      841 ggatcagcct gaccttctcg cagccggtca gttccttctc cagcgtccgg gccagctggt
      901 cgcgggccgt cgtctccaga aaaatggtgc aaatgttttc ggccttcatg gtgctgatga
      961 gattagccag ggcggcggcc gtcggttcgg ccagcgtgct gaccgagggg atgatcgtgc
     1021 cggcgatccg gaagccgtag gcatcggcca gatagccgag gctttcatga ttggtaatga
     1081 ggacgcgccg ctcggacggg atagcgtcga agcgggcacg caattcgccg tccagctgct
     1141 gaagttcggc tcgatagcgt tcggcgttgg ccgcgtaggt cgcctcgttg gcggggtcgg
     1201 ccgcgctcag cacctgccgg acgttatcga cccacaccat gacattgccc acatcctgcc
     1261 acacatgggg atcaaatggg ccatggtcgt cggcctcggc gccggcggtc atctttcgcg
     1321 gctcgatgcc tgccgatatg ggcacaatga tcgcctggtg cccgattgtg gtcaggtctt
     1381 tgaccagcgc ctcctcaagg ttccagccgt tgacgaagat cacattggcg tcggccgcgg
     1441 ccgccagatc ggccgcgccg ggctgataac tgtgggggtc ttgccccggc ctcatcagaa
     1501 ccgtcagatc gatggcgtcg ccgccgacct gatggacgac atcgccgatg atgcttgtcg
     1561 tcgccagcac ccgcaaccgt tcgccctcat gcgtccgtgc ggcgacgtcc ggcagcgcga
     1621 gaattccggc cgcgttcggc tccagggtgt tttcgtttga tgtgggcctg ttgccgctgc
     1681 aaccgatcag gccactgagc atcagcgcca acatgacatg ataaacgccc aaccgtcgac
     1741 gttgggcggg gtggatgtgg atcaattgtt actccagcgc cccatgtcgg gcgctattgg
     1801 ctaaatgata ttttgtatca ttataagaat agccgggatg ggtgtcaaat gtcgcgcgag
     1861 gatggaagtt tgcgggaaag agcatccgga ataattacgc tgcgatggtg tataatgaga
     1921 ttggttcaca gccttgctcg gcgagccgaa gggctggatg ctccgccatt cgatgagccg
     1981 gagcgcagcg tggccggagc aggcgtggcg cgcaagccct gacaagtggg cgttgaagtg
     2041 tcggagccgg cgggtcgccg gaaatggaag cagaaatgcc gggccggttg catgcgggag
     2101 gataaactga agtgagcgaa atgattcccg tcgagaacat tgaacgcgac gtggaggcgg
     2161 aagcgctggc cgtcgcctgg caggcagcta ctgtcgatta ctcgttcgtc cccctcccgg
     2221 atattaagga gcgctttctg cggctggctg cgtccgtgct gggcgcgatg ggggcggagc
     2281 cggatccggt tgcattgact gcgcacggcc ggagcatcgg ccgcgaactg gtcgagctga
     2341 atctgcataa gcccaaggtt cttgagaata cgcttgccgt cctgacggat cagctggccg
     2401 gcaagaacgt gcccggccaa ctggtcgcct tgatcagcgg catcgccacc ggtttcaccg
     2461 ccgccctgga agccctcctg ctgcgacaac aggagaatat caaccatgca gccactgcgg
     2521 ttctgaaaaa gacgcaggaa gacctgcact tctctcgcaa tcacctggct accgccaacc
     2581 gggagttgtc ggcgcagatc accgagcgta tccacgccga ggaaaggcag cgcgagttcg
     2641 ccgagcgctt gcagcatctg caccgaatcg atacggcgat tctgtcggcc gagtcgctgg
     2701 aagcgattgc cgacatcgcg atcaacttca tcagccacct gatcccatcc gtcctcatat
     2761 ctatctcgct cctggatttc gacgagcgtc gcctggaaat tatcggctcc accgatccgg
     2821 tccatttccc gcccgggcgg gaaatccccg tgatgctcct ggaagtattg gcgagacttg
     2881 aaaaggacgg tgcagcctat attgctgact ttgcgacgat cccaaacccc accccggtgg
     2941 caatcgagat cgccggattg ggcggtcatt cctctctgat cgtggccctt cgctatcagg
     3001 ggtggccgat cggcacgctg ggaatcacgc tgggcgaggt gcggccgttc agtgaggacg
     3061 agcagaccct ggctcgcgaa ctggccgcct cggtggccgt ggccctgcag catcgccgct
     3121 tgctccaggc ggagagagag gcacatgagc gcgaggcgac cctgcgcgaa gttgccgccg
     3181 ctctaaccct cgacctgagc ctggatgaag tcctcaatca tgtcctggct caactggagc
     3241 gggttatcac cagcgacagt tcctccatca tgctgatcag gggacatgat ctggaaatcg
     3301 cctgccagcg cggtgttgcg agcaaatatg aggatttgaa gaaattggtc aagctgcaac
     3361 ctcgcagcat ccggctggtg cttgagacgg cgcggccgaa tatcatcaat gagacaacaa
     3421 ccagccccga ttggattgtc gcgcccggcc gtgaatatat tcgctcctgg atgggcgtcc
     3481 cgctactggt gaagggcaat tgcatcggca tcatgaacct cgatcgccgc gatccgcacg
     3541 ctttcagcgc ccgtgacatg gagctggccg ccgccttcgc caatcaagcg gcagtggcca
     3601 tcgacaacgc ccgcctcttc gcccaccaac agcgatatgc cgaagagatg gaacagggcg
     3661 tgcgcgagcg aacccgggac ctggaagtcc tgtatggcat cacggcgacc gccgtcggca
     3721 acgccgacgt gaccgggttc ctgcaaggag ccatggggtt gacagccggc gcttttggct
     3781 gcgccaccgc cgccgcatat ctgagcgtgg gcaacgggtc gggtttgcaa ttagcggcgc
     3841 tgctggaggg cgggaacccc agcctggccg atttgctggc tgagcttggc gaggatagcc
     3901 ctctcttgcg tcgaccgctg gtcgatgggt tgccggaaat cctgttgggt agcgatctgc
     3961 cgccccaatg gggcgaatcg gcaggtttgg tgctggcaac cgtgccgtta cgctcgcgcg
     4021 gacaaagcct cggcgtgctg gctcttctct gtcatgagga cgcgcagttg acgagcgtgg
     4081 cccctgacct gttgacgacc atcgccgacc agattggcgc ggtggtggat aatattcgct
     4141 tgcatcagat cgcccggcaa tcggccatca tcgaggagcg cgaacgcatc gcgcgcgaca
     4201 ttcatgatca ggtgacgcag tccatctaca gcgccagcct gtttgccgag gcagcccgcg
     4261 gcgcggccga atccggcaac atggcaaagc tgcaacagca ccatcaat
//
LOCUS       DAOMQG010000300         4289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8545_length_4289_cov_10.248465, whole genome shotgun sequence.
ACCESSION   DAOMQG010000300 DAOMQG010000000
VERSION     DAOMQG010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8545_length_4289_cov_10.248465"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLC06_13360"
     CDS             <1..710
                     /locus_tag="PLC06_13360"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRO91480.1"
                     /translation="YPKDSTRKQVKGKYDLVYRLDRAGRLVDAQVFEYLEFSRDLFSE
                     ELLAELAGSCTQTVTIGEEKVVISHAYVQRRVIPLDIYVRDAHESAACAAVCDYGQAI
                     KDMASNNIFPGDMLLKNFGVTRHGRVVFYDYDELCLLEECNFRRLPPTARYEDDLAAE
                     PWFHVNPHDIFPEEFPHFLGLSGNLRDEFERRHAMLYTAEYWREQQEAVRAGVRSHIF
                     PYLPEQQLRGSTIEMNP"
     gene            707..1450
                     /locus_tag="PLC06_13365"
     CDS             707..1450
                     /locus_tag="PLC06_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRO91481.1"
                     /translation="MTLQELPITNLPASRVHAQVIVCGDPARADRIAEQLDGATLLSH
                     KREYRAHNGTYRGTPITVCSHGIGAAGAAIAFEELIVAGARTIIRVGTCGGLQSDVQP
                     GHLAIATAAVQNTGYGREVVPEGYPAVASAAATLALQQAVESSGRVARTGIVLSRDSF
                     YPGVDLATAPNYRVMSSANVLAVEMECAALFIVGSLRGARTAAILAVDGNVLEAVESV
                     EGYKPGRDVVHQAVDAAILVALEALCHLA"
     gene            1435..2229
                     /locus_tag="PLC06_13370"
     CDS             1435..2229
                     /locus_tag="PLC06_13370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO91482.1"
                     /translation="MPSRVSELQAQRRAIRHLLKENDAIDAMAAYYAFYHPAEKTTLI
                     VAPEGIEANPAAHADGYVALSRTGIDLFRPLVTMRLPIDRDPGAAEESADLLERALAP
                     GQPVILNIPVRYRPLIDALFDVQSEEPLRLYALEPAHFEPIVNVLVVRSTGSGGLPRF
                     SIRSNQNGEEEVVAAASLNWQSPHYAEIAVSTRPQYRRQGWGRSVVAALSGYLIENGR
                     APLYVVSEENSASAHLAESVGFTDTGAREYLLQASLKERARGVKKA"
     gene            2446..2661
                     /locus_tag="PLC06_13375"
     CDS             2446..2661
                     /locus_tag="PLC06_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRO91483.1"
                     /translation="MFRETGIVKWFSRLKGYGFINPDQGDKEVFVHYSAIRGDGYRNL
                     FEGDKVEYELIDRGKGPQAQNVQARQQ"
     gene            2817..3197
                     /locus_tag="PLC06_13380"
     CDS             2817..3197
                     /locus_tag="PLC06_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO91484.1"
                     /translation="MTYSILIVDDSLPLANLYQIVFERSQWLVDRVAGGQEALDKLAD
                     GIPDVILLDLMMPDMDGLEVCRRIRERLPQAPPCIAVYTAVNRPEARQDSLLAGADLF
                     FNKNLSIFDLPGQIAAHLQPPAAE"
     gene            complement(3214..>4289)
                     /locus_tag="PLC06_13385"
     CDS             complement(3214..>4289)
                     /locus_tag="PLC06_13385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRO91485.1"
                     /translation="DRQTFPRDKICGDAVSQGCIRIMNSLGMESKIEAAIARGELYQL
                     DGMRLVGPSGHTLEAHFTHGEDGAKSYVAPRIYYDAIIQQQAVESGVTFLQAEVKEPI
                     IEGGQVVGVLARVDGETKAYRSRVVVGADGVTSTIMRALRPEAGQHVDKHRAVALRAY
                     IKGLKLYPHEVEFFLYNEILPGYAWIFPISDDEANIGLGMRLDKFRKHKGNLKKMLKD
                     FLEMPAIRERAVNGTEIRDVAIWQLSFGSQKNLQHAYDGALLVGDAAGFINPLTGGGI
                     HNALISGILAADTIKEALRAGDYSRERLKIYETRCHEEMWDSMSKSFLYQRVLLNYPI
                     VVDFLIRYLGRHGGVAQTFLSKL"
BASE COUNT          864 a         1381 c         1189 g          855 t
ORIGIN      
        1 actatccgaa ggactccacg cgcaagcagg tcaagggcaa atacgatctg gtctatcgtc
       61 tcgatcgcgc cgggcgactg gttgacgccc aggttttcga atatctggag tttagccgcg
      121 acctcttctc ggaagagttg ctggcggagt tggccggctc ctgcacccaa actgtcacca
      181 tcggcgagga gaaggtagtc atctcccacg cctatgtcca gcgacgcgtg atcccgctcg
      241 acatctacgt ccgcgacgcc cacgaatcgg ccgcctgtgc cgccgtgtgt gattacggtc
      301 aggcgatcaa ggacatggcc tcgaacaaca ttttccccgg agatatgctg ctgaaaaact
      361 tcggcgtcac ccgccacggc cgcgtcgtct tctatgacta cgatgaactg tgtttgctgg
      421 aagaatgtaa cttccgccgg ctgccgccta ccgcccgcta tgaggacgac ctggccgccg
      481 aaccctggtt ccatgtcaac cctcacgaca tcttccccga ggaattcccc catttccttg
      541 gattatcggg gaacttgcgc gacgagttcg agcggcggca cgccatgctc tacacagccg
      601 aatattggcg cgagcagcag gaagccgttc gcgccggcgt tcgctcccac atcttccctt
      661 acctgcccga acaacaactt cgcggctcaa cgattgagat gaacccatga ccctgcaaga
      721 attacccatc accaacctgc ccgccagccg cgtccacgct caggtcatcg tctgcggcga
      781 cccggcacgc gccgaccgca tcgccgaaca actggacggc gcgacgctcc tgagccacaa
      841 gcgcgaatat cgcgcccaca acggtacata tcgtggtacg cccattaccg tctgctccca
      901 cggcatcggt gccgccggcg cggcaatcgc cttcgaggag ttgatcgtcg ccggcgctcg
      961 aaccatcatc cgcgtgggca cgtgcggcgg cttgcaatcg gacgtacagc ccggtcatct
     1021 ggccatcgcg acggccgccg ttcaaaatac cggctacggc cgcgaagtcg tccccgaagg
     1081 ttacccggcc gtggcgagcg cggcggccac gttagccctg cagcaggccg tcgaatcctc
     1141 cggccgggtg gcccgaacgg gcatcgttct ctcccgcgac agcttctacc ccggcgtcga
     1201 tttggctacc gcgcccaact accgggtcat gtccagcgcc aacgtcctgg cggtggagat
     1261 ggaatgtgcc gccttgttca tcgtcggcag cctgcgcggg gcacgaacgg cggccatcct
     1321 ggccgtcgac ggcaacgtgc tggaagcggt cgagagcgtt gagggctaca aacccggccg
     1381 ggacgtcgtt caccaggccg tggatgcggc catcctcgtc gcgctggagg ccctgtgcca
     1441 tctcgcgtaa gcgaactcca ggcccaacgt cgcgccattc gtcacctgct gaaggaaaac
     1501 gatgcgatcg acgccatggc cgcctactac gccttctacc atcctgccga aaagaccact
     1561 ttgatcgtcg cgccggaagg catcgaggcc aaccccgcgg cccatgccga cggctacgtg
     1621 gcgttgtccc gcacgggaat cgatctgttc aggccgctgg tgacgatgcg gctgccaatc
     1681 gaccgcgatc ccggcgctgc cgaggaatcg gccgatctgc tggagcgcgc cctcgccccc
     1741 ggccagcccg tcatcctcaa tatccccgtt cgctatcgcc ccctcatcga cgcgctgttt
     1801 gatgttcagt ctgaagagcc actgcgcctc tacgcgcttg agccggccca ttttgagccg
     1861 atcgtcaacg tgctggtcgt gcgctccacc ggctccggcg gcctgccccg attctccatt
     1921 cgctccaatc agaacggcga ggaggaagtg gtggccgcag catcactcaa ctggcagtct
     1981 ccccattatg ccgaaattgc agttagtacc cggccacaat accgccggca gggatggggg
     2041 cgcagcgtcg tcgcggccct cagcggctat ctgattgaga acggccgcgc cccgctctac
     2101 gtcgtctccg aagaaaacag cgcctctgcc cacctcgccg aaagtgtcgg gttcaccgat
     2161 acgggcgcgc gcgagtactt gctccaggct tccttaaaag agcgtgcaag aggcgtgaag
     2221 aaggcgtgaa atatgaatca cgcccgttga cccactgtcg gcaggtatga taatctgcct
     2281 gtaacttcac ttgtatcggt atcttcggta aagccacgta ttggtaattg ctgcggataa
     2341 agcaccatct ccgtccctcc ggaatggtgc tttattcgga gcttatcccc gacatttgaa
     2401 taaagccaac agtatttgca gacccactgt ggaggtaatg gtaatatgtt tcgcgagaca
     2461 ggtattgtca agtggttcag ccgattgaag ggctacggct tcatcaatcc ggatcaagga
     2521 gacaaggagg tcttcgtcca ctattcggct atacgcggcg acggctatcg caacttgttc
     2581 gagggagaca aggtggagta cgaactgatc gatcgcggca aaggcccaca ggcgcagaac
     2641 gttcaggccc gtcagcagta agctcgcgaa catccctcac gggagcagta tgttcgacgt
     2701 attggaagat atgcccgcca cccggcgggc ttttttataa cctgcagcat tagcaaacaa
     2761 tcgttccggg agatacaata tcaacaagtc agcctgacgg atccggactc catgccatga
     2821 catattccat cctgatcgtc gacgatagtc tacctttagc caatctctac cagattgtgt
     2881 tcgagcgcag ccagtggctg gttgatcgcg tggccggcgg gcaggaagcc ctggacaagc
     2941 tggccgacgg catccccgac gtcatcctgc tcgatttgat gatgccggat atggatgggc
     3001 tggaggtatg ccgccgcatc cgcgaacggc tgccgcaagc cccgccttgc atcgcggtct
     3061 atacggccgt caatcgcccc gaagcgcgcc aggacagcct gctggccggg gccgacctgt
     3121 tcttcaacaa aaacctgtcg atcttcgatc tgccggggca gatcgccgcc cacctccaac
     3181 cgccggccgc tgaataatct acatcgtccc aagctagagc ttggagagga acgtctgcgc
     3241 cacaccgcca tgccgtccca ggtagcggat caggaaatcc acgacaatgg ggtaattcaa
     3301 cagcacccgc tggtacagga aggatttgct catgctgtcc cacatctctt catgacaacg
     3361 cgtctcgtag atttttagtc gctcgcgaga ataatcaccg gcgcgcagag cctccttgat
     3421 ggtgtcggcc gccagaatgc cggagatcag cgcgttgtgg atgccgccgc cggtcaacgg
     3481 gttgatgaac ccggccgcat cccccaccag caacgcgcca tcataggcat gttgcagatt
     3541 tttctgtgag ccaaagctca gttgccagat cgccacgtcg cgtatttccg tcccgttgac
     3601 ggctctctcg cgaatggccg gcatctccag gaaatccttc agcatcttct tcagattgcc
     3661 cttgtgcttg cgaaacttgt ccagccgcat accaagcccg atgttggcct cgtcatcgct
     3721 gatggggaaa atccaggcat agccaggcaa gatctcatta tagaggaaga attccacttc
     3781 atgagggtag agtttgaggc ccttgatgta ggctcgcagc gcgactgcac gatgcttgtc
     3841 gacgtgttgc cccgcttccg gccgcaaggc gcgcataatc gtcgatgtga ccccgtccgc
     3901 gccgacaaca acccgcgagc gataggcttt tgtctcgcca tccacgcgcg ccaacacacc
     3961 cacaacctgg ccgccttcga taatcggctc tttcacctcg gcttgcagaa aggtcacccc
     4021 gctttccacg gcttgttgct ggataatggc atcgtagtag atacgcggcg ccacatagga
     4081 tttggcaccg tcttcaccat gggtgaaatg ggcttccaga gtatgaccgc tggggccaac
     4141 aagccgcatc ccgtcgagtt ggtacaattc gccgcgagct atggctgcct cgatcttcga
     4201 ttccattccc aggctgttca taatacgaat gcaaccctgc gagacggcgt cgccgcagat
     4261 cttgtctcgc gggaaagttt ggcgatcaa
//
LOCUS       DAOMQG010000301         4289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8546_length_4289_cov_10.139584, whole genome shotgun sequence.
ACCESSION   DAOMQG010000301 DAOMQG010000000
VERSION     DAOMQG010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_4289_cov_10.139584"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLC06_13390"
     CDS             <1..252
                     /locus_tag="PLC06_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRO91486.1"
                     /translation="PLLRTWLSWWRDLALMTYGRGSNEAISNVDESGKLHELAGRLSR
                     ASVFASLAQTEAALRQLGQNANARLVLENVMLTYPSRTE"
     gene            275..838
                     /locus_tag="PLC06_13395"
     CDS             275..838
                     /locus_tag="PLC06_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91487.1"
                     /translation="MNKRFPLGKLGRLDITAEPSAAGGFLLLGVVFGLLGVKLFRLKP
                     GAAAAGGLLAAALHFIAEMWHQLGHARAAEESGYPMEGVHLWGVLGTSMYPDDEPELP
                     AEVHVARALGGPKASAGLAAVAGLLAALVHPFSRLAFMIASIFALENSLIFTIGAFLP
                     LPFMETDGTTLQHYRAEYRKRMIIIQE"
     gene            849..1646
                     /gene="hisN"
                     /locus_tag="PLC06_13400"
     CDS             849..1646
                     /gene="hisN"
                     /locus_tag="PLC06_13400"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRO91488.1"
                     /translation="MSNGEPTLDELLNFALDAAWQAGRVTLGYFQTGTQVERKADNTP
                     VTIADREAEQTLRRLITERWPGHALIGEEYGRAAGNAGDSGYTWIIDPIDGTKSFVQG
                     VPFYAVLLALVKDDQPLLGVMYFPALNEIVYAARGRGCYWNGRPAHVSTVADLKDAVL
                     LASDLDTFARFGREHSFQRLIDATYIQRTWGDAYGYALIATGRAEVMVDPVMEVWDCG
                     PLQVILEEAGGTFTDWKGTPTIFNREAVATNGVLFDRVMAIIREGEA"
     gene            1803..>2715
                     /locus_tag="PLC06_13405"
     CDS             1803..>2715
                     /locus_tag="PLC06_13405"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HRO91489.1"
                     /translation="MSKKNPAKPVSRLRLGLMAVLVCALMALAACRGSAGEAVATPTV
                     GPTVESTFISTETVRGQAIVNSIDVQILESFPVQINVVARGDLPDSCTQIDEIITQQT
                     DNAFRVAITTLRGPAASCTMSLVPFEQSIPLDVAGLPAGRYDVSVNGVQGAFTLDVDN
                     VLPDDATTGAGAAIQTEPSISGLVWHDLCSQTGTPAEGTTPADGCVVARTGNTLQANG
                     VYDQGEPGIPGITMRLLAGDCTTAGEGDEIVATTDESGSYRFENVTADTYCVFLDTAD
                     EENATILEEGILTFPLSNGVGTNSTTIT"
     assembly_gap    2716..2815
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2816..3766
                     /locus_tag="PLC06_13410"
     CDS             <2816..3766
                     /locus_tag="PLC06_13410"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRO91490.1"
                     /translation="ILTFPLSNGVGTNSTTITLEEGAALKDINFGYDYRFLPVPDVAE
                     NCTNSFEFIQDLTVTDNTVFPPGAEFEASWRLRNNGTCPWTKDYAVAFIGGDAMGITS
                     TVKLESPVAPGQTKDVGITLTAPAAPGTYRSNWQLSDANGTVFGINGAIEDAFWVQIV
                     VEEGAEFTPPSAGSSVIGGVVWDDICFMTNGVASRGCVETAEGSGFFRGNGSFDANES
                     PLSGITVILGRGACPEGGVPTPANRLATAQTDEKGLYRFEGLDADIYCVAIDALSSEN
                     VNLLIPGNWTWPAPGTGRQGIRLAAGEQRLTVDFGWDFSQ"
     gene            3898..>4289
                     /locus_tag="PLC06_13415"
     CDS             3898..>4289
                     /locus_tag="PLC06_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91491.1"
                     /translation="MNNTKPVSWRGVSAGEKLILTILPLFAIAVMVLPFLDQVILRRL
                     ARLPGMRPVVIWLVNGFNVWIAGLGLLLLFGLFALFVRHRLISNRKLWFGTGCPQCME
                     RDLVRVSRQTSDRFYGLIGVPAYRYACR"
BASE COUNT          820 a         1264 c         1268 g          837 t
ORIGIN      
        1 ccgctcttgc gcacgtggct cagttggtgg cgcgatctgg cgctcatgac ctacggtcgc
       61 ggatcaaacg aagccatcag caacgtggac gagtccggga agctccatga attggccggt
      121 cgcctgtcgc gggcttctgt attcgcgtct ctggcgcaga cggaagcagc tttgcgccag
      181 ctcgggcaga acgccaacgc gcgtctggta ctcgaaaacg tgatgctgac ctatccatct
      241 cgcacggaat aagtaacgac aagtgagcga attgatgaac aaacgatttc ccttgggcaa
      301 gctcggcagg ctcgacatca ccgccgagcc gtcggccgcg ggcggattcc tccttctggg
      361 ggtcgtattc ggcttgctgg gcgtgaagtt gttccggcta aaacccggcg cggcggcagc
      421 gggcgggctg ctggcggccg cgctccactt tatcgccgag atgtggcatc agcttggcca
      481 cgcccgcgcc gctgaggaat cgggctatcc tatggaaggc gtccatttgt ggggcgtgct
      541 gggcacctcg atgtacccgg acgacgagcc tgaattaccc gcggaagtcc acgttgcccg
      601 agcgctgggc ggcccaaaag ccagcgccgg gttagcggcc gtggccgggt tgctcgccgc
      661 gctggttcac ccgttcagcc ggttggcttt catgattgcc tcgatcttcg ctctggaaaa
      721 ctcgctcatc ttcaccattg gcgcgttcct gccgctacct ttcatggaga cggacggcac
      781 gacattgcag cattatcggg cggaatatcg caagcgcatg atcattattc aggagtagga
      841 caaatagaat gagcaacggc gaacccacac tcgatgagct gctcaatttc gccctcgacg
      901 ccgcctggca ggcggggcgc gtcaccctgg gctatttcca gaccgggacg caagtcgagc
      961 gcaaggcgga taacacgccg gtcaccatcg ctgatcggga agcggaacaa acattgcgcc
     1021 ggctcattac cgagcgctgg ccgggccatg ccctcatcgg cgaggaatat ggtcgtgcgg
     1081 cgggcaatgc cggcgatagc ggctacacgt ggatcatcga tcccatcgac ggcacgaaat
     1141 ccttcgtcca gggggtaccg ttctacgccg tcctgctggc cctcgtgaaa gacgaccaac
     1201 ctttgctggg ggtgatgtat ttccccgccc tgaacgagat cgtctacgcg gcgcgtggtc
     1261 gtggctgcta ctggaacggc cgcccggctc atgtttcaac cgttgccgat ctcaaggacg
     1321 ccgtcttgct ggccagcgac ctggacacct tcgcccgctt cggtcgggag cacagcttcc
     1381 aacgtctgat cgacgccact tacatccagc gcacctgggg tgatgcatat ggatatgcgc
     1441 ttatcgccac cggtcgggcc gaggtcatgg tcgatcctgt catggaggtc tgggattgcg
     1501 gcccattgca agtgatcctt gaggaagcgg gcggcacctt caccgattgg aaggggaccc
     1561 caacgatctt taaccgcgag gccgttgcca ccaatggcgt gttgtttgac agggtaatgg
     1621 ctatcatccg tgagggagaa gcctgaacca acgaccaggc cttgacaagg agcgatcggc
     1681 tatcgggaga tgctcctgta taattcacag agccaaaaca tataattggc aggaaaaggg
     1741 aacgtccacc gttcaagcac ctgcatcata gcccaggatg tggactttag aggagaaaaa
     1801 gtatgagcaa gaaaaaccca gcaaagccgg tctcgagatt acggctgggc ctgatggccg
     1861 tcctggtatg cgcgctcatg gccctggccg catgcagagg ttctgccgga gaagcggtcg
     1921 ccacgccgac ggtcggccca actgtggagt ctaccttcat ctcaaccgag acagtccgtg
     1981 gccaggcgat cgtcaacagc atcgatgtgc agattctgga gtcattcccg gtgcagatca
     2041 acgtcgtcgc ccgtggcgat ctgcccgact cctgcaccca gatcgacgag atcatcaccc
     2101 agcagacaga caacgctttt cgcgtcgcca tcaccaccct tcgcgggccg gccgcaagtt
     2161 gcaccatgtc gcttgtcccg tttgaacagt cgatcccgct cgacgtcgcc gggcttccag
     2221 ccggacgcta tgacgtctcg gtcaatgggg tgcaaggcgc cttcaccctc gacgtagata
     2281 atgtcttgcc cgacgacgcc accaccggcg cgggggccgc gatccagact gagccaagca
     2341 tcagcggcct ggtgtggcac gatttgtgca gccagaccgg cacaccagcc gagggcacaa
     2401 ccccggccga tggctgcgtg gtcgccagga cgggcaacac cttgcaggcc aatggcgtgt
     2461 acgatcaagg tgagcctggt atccccggca tcaccatgcg tctgctggcc ggagattgca
     2521 ccacagccgg cgaaggtgac gagattgtcg ccacgaccga cgaatcaggc tcataccggt
     2581 tcgagaatgt gaccgccgac acctattgcg tgttccttga cacggccgac gaggaaaacg
     2641 cgaccattct ggaagagggc attctgacgt tccccctcag caacggcgtc ggcaccaaca
     2701 gcaccaccat cacccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattct
     2821 gacgttcccc ctcagcaacg gcgtcggcac caacagcacc accatcaccc tcgaagaggg
     2881 tgcggcgctc aaggatatca acttcggcta tgactaccgg ttcctgcccg tgcccgacgt
     2941 ggccgaaaat tgcaccaata gcttcgagtt catccaggac ctgacagtga ccgacaatac
     3001 tgtgttccca ccgggggcgg aattcgaggc atcctggcgc ttgcgcaata acggcacgtg
     3061 tccgtggacc aaggattacg ccgtggcttt cattggcggc gatgcgatgg gcatcacgtc
     3121 caccgtgaaa ctggaaagtc cggtcgctcc agggcagacg aaagatgtgg gcatcaccct
     3181 gaccgcgccg gcagcgccgg gtacctatcg cagtaactgg cagctcagcg acgccaacgg
     3241 caccgttttt ggcatcaacg gcgccatcga ggatgcgttc tgggtgcaga tcgttgtaga
     3301 agaaggggcc gaattcaccc caccgtccgc cgggtcgtcg gtcatcggcg gcgtcgtgtg
     3361 ggatgacatc tgcttcatga ccaacggcgt tgcctcgcgg ggttgcgtcg agacggccga
     3421 aggcagcggc tttttccgtg ggaacggctc attcgacgcc aacgaatcgc cgctttcggg
     3481 catcaccgtg atcctgggcc gcggcgcatg ccccgaaggg ggtgtgccca cgccggctaa
     3541 ccgcctggcc acggcccaga ccgatgagaa gggcctttat cgttttgagg gtctggatgc
     3601 cgacatctac tgcgtagcca tcgatgcgct cagttccgag aacgttaacc tgctgatccc
     3661 cggcaactgg acctggcccg ccccgggaac cggccggcag ggcattcgcc tggctgcggg
     3721 cgagcaacgt ctgaccgtcg atttcggttg ggactttagc caataaacga aagcggggag
     3781 ccggacatcg tgccagatat ccggctccct gtatcggtcg caacgggtta aggtttttcg
     3841 taagcggcat cgcctaccat acgggatggt gggaattttt tgtgggagtc tgcaggattg
     3901 aacaatacca aacctgtctc ctggcgtggt gtcagcgccg gagagaaact tatattgacc
     3961 attctgccgc tattcgcgat cgcagttatg gttctgcctt tcctcgatca ggtgatcctg
     4021 cgccggctgg ctcgtttgcc gggcatgcgg ccagtagtca tctggcttgt caatgggttt
     4081 aatgtctgga ttgccggttt ggggttgcta ttgctttttg gccttttcgc tctattcgtc
     4141 cgtcaccggc tgattagcaa caggaagctc tggtttggca ccggatgccc acaatgcatg
     4201 gagcgcgatc tggtgcgagt atcgcgtcag actagcgatc ggttttatgg tcttatcggg
     4261 gtgccggcct atcgctacgc ttgtcgcaa
//
LOCUS       DAOMQG010000302         4252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8648_length_4252_cov_6.235883, whole genome shotgun sequence.
ACCESSION   DAOMQG010000302 DAOMQG010000000
VERSION     DAOMQG010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8648_length_4252_cov_6.235883"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..724
                     /locus_tag="PLC06_13420"
     CDS             35..724
                     /locus_tag="PLC06_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91492.1"
                     /translation="MSDRLFFFSGLLGLGTLAVTLVLSLVGPGEIGPMAPGFNSPVLA
                     FEFAESEAEVRALFEPAGSAAAMDRVNRWDFLYMVLYSLFLAVFALAAALETDQYTPW
                     IAAVLPLVILFADVMENVQLFRLTAQLGLDGGSMDPALGRLHLFTWLKWGGLALYFVL
                     ISGYFREQPGLWRFVWVTADIPALLAIWAFFWRGQVHEYMALSIGLLFLLLVVFAWWR
                     ALTSGLQARGV"
     gene            950..3622
                     /locus_tag="PLC06_13425"
     CDS             950..3622
                     /locus_tag="PLC06_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRO91493.1"
                     /translation="MEFWLNGELKQYDGDGELPLLTWLRLDQGITSAKDGCAPQAACG
                     ACAVDLNGKAVLSCVTAMKRLAGGRVTTIEGLGEYRQNVYANAFVSKGGVQCGYCIPG
                     IVIQANALINKNNIPTRDEIAKALTPNLCRCTGYKKIIDSVVLAAEAIRKEEEVPPPV
                     PDGRVGGRHPKYHAEDLVLGRHNYVGDIYLPGMVYGALRFSDHPRANVLSIDTGPAEA
                     LPGVIRVVTASDVPGDRTIGLIRQDWPLMIAVGETTRYVGDVLAGVVAETEAIARAAA
                     ALIRIEYEVLEPVTDMHKALLPDAPQIHATGNQLSRTAFTRGDLDAARAKTVYTATGV
                     YQTQRIEHGYMEPECAIAIPTEGRSTAYPLNTDSLNTDLLTTDHSPLPAAEVVHVLSQ
                     SQGVYDDQIQIAKLLDVPREAVRVTLVPNGGGFGGKEDLSVQGHAAVMAWLTGRTVRV
                     GLTRDESIAMHPKRHPIWMDYTVGCDAGGKLTYVKVKFVGDTGAYASVGMKVLERAAG
                     HATGAYHVPAADIEATAAYTNNIPCGAMRGFGVNQAAFGLESCLDQLCEMGGFDRWQF
                     RYDNALVNGNVTSTGQIIEGGAGARETLLAVKEQFYAAKYAGIACGIKNTGVGNGMAD
                     SSHVSLTIESAERVILDHGWTEMGQGVHTIAVQTLCNETGIDPRIVQVRVDTSMKQEA
                     GMTTASRATSLVGNATINAAAGLKEDLKTHTLAELAGRVYKGAWIVDWTTKPGYEKNG
                     KTITHYSYSYATQLVVLDDDGQISKVTAAHDAGKIYNPTLFEGQLEGSIHMGLGYAIS
                     EEMPMENGRPVSTRLRQMGILRAKEMPEMEIIGIEVPDPYGPYGAKGVGEIGLVPTAA
                     AVANALYQFDGIRRTRLPMELPRREKVVVKKSDE"
     gene            complement(3675..>4252)
                     /locus_tag="PLC06_13430"
     CDS             complement(3675..>4252)
                     /locus_tag="PLC06_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRO91494.1"
                     /translation="VGMWGHSMGGYITLRAMVVSDEIDAGVIWGGVVAPYPDLFNRAG
                     ALATAVAADAPAVTATAAAAQATTAAAQATATARALEGTPAFRFGRWRSSLIDTYGTP
                     EENPAFWDSISSNAYLADISGPLQLHHAITDETVPAAASVMLHDQMTALGLPVELYLY
                     DNDSHDIDNNFYTAMRRSLEFFDLYVKAVTE"
BASE COUNT          750 a         1372 c         1428 g          702 t
ORIGIN      
        1 tccgtcgggc ggcagacttt ccaaaacctg aatcatgagc gatcggctat tctttttcag
       61 tggactcctc ggccttggca cgctggcggt gacgcttgtc ctgtcgctcg ttggccccgg
      121 cgagatcggg ccgatggcgc ccggtttcaa ctcccccgtc ctggcctttg aattcgccga
      181 gagcgaggcc gaagtccgcg ccctgttcga gccggccggt tcggccgcgg cgatggatcg
      241 cgtcaaccgc tgggactttc tctacatggt gctctacagc ctgtttctgg ccgtcttcgc
      301 cctggcggcc gcgctggaga ctgatcaata tactccctgg atcgccgccg tcctgccgct
      361 ggtgatcctg ttcgccgacg tgatggagaa cgtccagttg ttccggctga cggcgcagct
      421 gggcctcgac ggtggcagca tggaccccgc gctcggccgc ctccatctct tcacgtggct
      481 caagtggggc ggactggcgc tctattttgt gctgatctcc ggctatttcc gcgagcaacc
      541 cggcctctgg cgcttcgtct gggtgacggc cgacatcccc gcgctgctgg ccatctgggc
      601 tttcttctgg cgcggccagg tgcatgagta catggccctg tccattggcc tgctgttcct
      661 gctgctggtc gtcttcgcct ggtggcgcgc gctgacgagt gggttgcagg cgaggggagt
      721 gtagggggaa ggggagaagg ggagaagggg agaagggggg aaggggtgtg ttcgtgggcg
      781 gtgggtcggt gggtcagtga tcagtgggtc agtggggcag tggccgcgcg acgggagtcg
      841 cgttggcgcg acgggagtcg cggggcgcga cgggagtcgc atcttaatct ttaattcgta
      901 attcgtaatt cgtaattcgt aattcgtaat ttattcaagg agtttggcaa tggaattctg
      961 gttgaacggc gaactgaaac aatatgacgg ggacggagag ctgccgctgc tgacctggct
     1021 gcggctggat cagggcatca cctcggccaa ggacggctgc gcgccccagg cggcttgcgg
     1081 cgcctgcgcc gtcgacctca acggcaaggc ggtgctgtcc tgcgtcacgg cgatgaaacg
     1141 gctcgccggc ggccgcgtca ccaccatcga gggtctcggc gaataccgcc aaaacgtcta
     1201 cgccaacgcc ttcgtcagca agggcggcgt gcaatgcggc tactgcatcc ccggcatcgt
     1261 catccaggcc aacgccctca tcaacaaaaa caacatcccc acccgcgacg aaatcgccaa
     1321 ggccctaacc cccaacctgt gtcgctgcac cggatacaag aaaatcatcg actcggtggt
     1381 gctggccgcc gaagccatcc gtaaggaaga ggaagtgccg ccgcccgtgc ccgacggccg
     1441 cgtcggcggg cgccatccca aataccacgc cgaagacctg gtgctcggcc gccataacta
     1501 cgtcggcgac atctacctgc cgggcatggt ctatggcgcg ctgcgcttca gcgaccaccc
     1561 gcgggccaat gtgctgagca tcgacaccgg cccggccgag gcgctgcccg gcgtcatccg
     1621 cgtagtcacc gcatcggatg tgcccggcga ccgcaccatc ggcctcatcc gccaggactg
     1681 gccgctgatg atcgccgtgg gcgagacgac gcgctatgtg ggcgacgtgc tggccggggt
     1741 ggtggccgag accgaagcca tcgcccgcgc cgcggccgcg ctcatccgca tcgagtacga
     1801 ggtgctggag ccggtgaccg acatgcacaa ggcgctgctg ccggacgcgc cgcagatcca
     1861 cgcgaccggc aaccagctct cgcgcaccgc tttcacccgc ggcgacctcg atgccgcccg
     1921 cgccaaaacc gtctacaccg ccaccggcgt ctatcagacc cagcgcatcg agcacggcta
     1981 catggagccg gagtgcgcga ttgccattcc gaccgaaggt cgctcgaccg cttatccatt
     2041 gaacactgac tcactgaata ctgacctact gaccactgac cactcaccac tgcccgctgc
     2101 cgaagtcgtc cacgtcctgt cgcagagcca gggcgtctat gacgaccaga ttcagatcgc
     2161 caagctgctg gacgtgccgc gcgaggcggt gcgggtgacg ctggtgccca acggcggcgg
     2221 cttcggcggc aaggaagacc tgtcggtgca gggacacgcg gccgtcatgg cctggctgac
     2281 cgggcgcacg gtgcgcgtcg ggctgacgcg cgacgagtcg atcgccatgc accccaagcg
     2341 ccatcccatc tggatggatt acaccgtggg ctgcgacgcc ggtggcaagc tgacctacgt
     2401 caaggtgaag ttcgtcggcg acaccggggc ctatgcctcg gtcggcatga aggtgctgga
     2461 acgcgccgcc ggccacgcca ccggggccta tcacgtgccc gcggccgaca tcgaagccac
     2521 cgccgcctat accaacaaca tcccctgcgg ggccatgcgc ggcttcggcg tgaatcaggc
     2581 cgccttcggg ctggagagct gtctcgacca gctgtgcgag atgggcggct tcgaccgctg
     2641 gcagttccgc tatgacaacg cgctggtgaa tggcaacgtc acctcgacgg ggcagatcat
     2701 cgagggcggc gccggcgcgc gcgagacgct gctggccgtg aaagagcagt tctacgccgc
     2761 caaatatgcg ggcatcgcct gcggcatcaa gaacaccggc gtgggcaacg gcatggccga
     2821 ctcgtcccac gtgagcctca ccatcgaatc ggccgagcgg gtcatcctcg accacggctg
     2881 gacggagatg ggccagggcg tccacaccat cgccgtgcag acgctgtgca acgagacggg
     2941 catcgacccg cgcatcgtcc aggtgcgcgt cgatacgtcg atgaagcagg aagcgggcat
     3001 gaccaccgcc agccgcgcca cttcgctggt gggcaacgcc accatcaacg ccgccgccgg
     3061 tctgaaggaa gacctgaaga cccacacgct ggccgagctg gccgggcggg tctacaaagg
     3121 ggcgtggatc gtcgactgga cgaccaagcc gggctacgag aagaacggca agacgatcac
     3181 ccactactcc tacagctacg ccacgcagct ggtggtcctc gacgacgacg gccaaatcag
     3241 caaggtgacg gccgcccacg acgcgggcaa gatctataac ccgacgctgt tcgagggaca
     3301 gctggaaggc tccatccaca tgggcctcgg ctacgccatc agcgaggaga tgcccatgga
     3361 gaacggccgc ccggtcagca cgcgcctgcg gcagatgggc atcctgcggg ccaaggagat
     3421 gccggagatg gagatcatcg gcatcgaggt gcctgacccc tacggcccct atggcgcgaa
     3481 aggtgtgggc gagatcggcc tcgtgccgac ggccgcggcc gtggccaacg cgctctatca
     3541 gttcgacggc atccgccgga cacggctgcc gatggagctg ccgaggcggg agaaggtggt
     3601 ggtgaagaag agcgatgaat gacgagtgac gagtgggaca tcggcggttg gtggtcgatt
     3661 tcgtccttcg cccttcattc ggtcactgcc ttcacatata aatcaaaaaa ctccagcgac
     3721 cggcgcatgg cggtgtagaa gttgttgtcg atgtcgtggg agtcgttgtc gtagaggtat
     3781 agctccaccg gcaggccgag ggcggtcatc tggtcatgga gcatcaccga cgcggccgcg
     3841 ggcacggtct cgtcggtgat ggcgtggtgg agctgcagcg gcccggagat gtcggccaga
     3901 taggcgttgg aggagatgga atcccagaag gcggggttct cttccggcgt gccataggtg
     3961 tcgatcaggc tgctgcgcca gcggccgaag cggaaggcgg gcgtgccctc aagcgcgcgg
     4021 gcagtggccg ttgcctgggc ggcagcggtc gttgcctggg cggccgcggc agtagctgtc
     4081 acggccggcg cgtcggcggc caccgccgtg gccagcgccc cggcgcggtt gaacaggtcg
     4141 ggataggggg ccacgacccc accccagatg acccccgcgt cgatctcgtc cgacacgacc
     4201 atcgcccgca gggtgatata gccgcccatg ctgtggcccc acatgcccac gc
//
LOCUS       DAOMQG010000303         4248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8664_length_4248_cov_11.313379, whole genome shotgun sequence.
ACCESSION   DAOMQG010000303 DAOMQG010000000
VERSION     DAOMQG010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8664_length_4248_cov_11.313379"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLC06_13435"
     CDS             complement(<1..1181)
                     /locus_tag="PLC06_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HRO91495.1"
                     /translation="MKFDFGREVCGDLPTATRREWLVTNGIGGFASGTIANVLTRRYH
                     GLLMAALNPPLGRTLLLAKLDETAEYDGIYPDSGRFYPLYTNRWEDGTVDGNGHVYLD
                     RFHLEGTTPVWTFALANALLEKRVWMQPGANTTYVQYRLTRATGPLSLSIKALVNYRD
                     YHATTIINDWDPEMKSLTVPGGSGLRIRMFDEAAPFYLFSDRAEITPQFDWYEDLFLS
                     VEEYRGQRDVSEDHIYAAQLQITLRQGETFAIVASTDGAPTLDSAAAWEERRDYERGL
                     LERAAGLRSNAQTKLPRPLEQLVLAADQFVVSRPSAADPDGKSIIAGYHWFSDWGRDT
                     MIALPGLTLTTGRPEVAAGVLRTFARFVDRGMLPNRFPDAGETPEYNTVDATLWYFIA
                     L"
     assembly_gap    1492..1591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1592..2463
                     /locus_tag="PLC06_13440"
     CDS             <1592..2463
                     /locus_tag="PLC06_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91496.1"
                     /translation="SATPAPTSIRTDLDAAERIVTDGGFAFRLFLPDLDVELQPTQAL
                     FADKKGTFLSSMGGGVEADDSDSLDIILSDILQSLNESMAAGLQAGEPYPITVGGVEG
                     LAVDVSGTMFDGPVLGRLLAVRPYKGQIFYAFALGNTASGDERWVEDGSSYFETLLDS
                     LRFFPIALGGEDSLTPAPAAGSDGGPCPVSVDETYGYTQENAVRVGGGAFGGPSRAIA
                     YLDTLQGPAGEAVTYHRIGSMPFEDTILDSYEVSYEGSAQPAVLFVDQYAFETLYAPV
                     GFTCKIPFPLTAP"
     gene            2511..3203
                     /gene="nth"
                     /locus_tag="PLC06_13445"
     CDS             2511..3203
                     /gene="nth"
                     /locus_tag="PLC06_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRO91497.1"
                     /translation="MTKSLVTGHQGETSEHVTQIIARLRATYPDAHCELDYETPIQLL
                     VATILSAQCTDARVNMTTPALFERFPTVETLAGADPVEMEELVRPTGFYRQKARYIRE
                     AATRILTEFGGEVPSDLDDLLTLPGVARKTANVVRGEIFGLSDGITVDTHVKRLAARL
                     GLTTATDPVKVERDLMAIIPRESWREISHLLIWHGRRLCTARKPACHECPLNDICPSA
                     FKPADYRPGKND"
     gene            3460..4191
                     /locus_tag="PLC06_13450"
     CDS             3460..4191
                     /locus_tag="PLC06_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRO91498.1"
                     /translation="MGTILSDGPVSYYQQLYTILRDELTRGKWKPGERMPSETELIAA
                     YGVSRITVRQAFDLLVNDGFVYRRRGSGTFVTARSSAPSLNRILSFTEDMEQRGLRPE
                     TRPIAARLEPAAADVARKLGIEPGEELVVLESLCLADDAPMSVERSHLVHRHCPGLLD
                     GDYARVSLHRTLLERYGIRLARANQAVWAVAAGAETAAALSVLPGAPLLCIEQVLFRQ
                     TGAPVEYLQAYRRGDRYVLYNELRA"
BASE COUNT          738 a         1226 c         1330 g          854 t
ORIGIN      
        1 tgcagggcga tgaagtacca cagcgtggcg tcgaccgtgt tgtattccgg cgtctcaccc
       61 gcgtcgggaa accgattggg taacatgccc cggtcgacga accgggcgaa ggtgcgcagg
      121 acgcccgcgg ccacttccgg acggccggtg gtcagggtca ggccgggcag ggcgatcatc
      181 gtgtcgcgcc cccagtcgct gaaccagtgg tagccggcga tgatcgactt gccgtcgggg
      241 tcggccgcgg aggggcgact gacgacgaac tggtcggcgg ccagcaccag ttgctcaagc
      301 ggccgcggca gtttcgtttg cgcgttgctg cggaggccgg cggcgcgttc cagcaagcca
      361 cgctcgtagt cgcgtcgctc ttcccaggcg gcggcgctgt cgagagtggg cgcgccgtcg
      421 gtgctcgcca cgatggcgaa cgtctcgccc tgtcgcaagg tgatctgcaa ttgcgcggcg
      481 tagatgtggt cctcgctcac gtcgcgctgc ccgcgatact cctcgacaga caggaacagg
      541 tcctcgtacc agtcgaactg cggggtgatt tcggcgcggt cgctgaacag atagaagggc
      601 gcggcctcgt cgaacatgcg gatgcgcaag ccgctgccgc cagggacggt taaggacttc
      661 atttcggggt cccagtcgtt gatgatcgtg gtggcgtggt aatcgcgata attgaccagc
      721 gccttgatcg acaggctgag cgggccggtg gcgcgcgtca accggtactg cacataggtg
      781 gtgttcgcgc cgggctgcat ccagacgcgc ttttccagca gcgcgttggc cagggcgaag
      841 gtccacacgg gggtggtgcc ttccagatgg aagcggtcga gatagacatg gccgttgccg
      901 tcgacggtgc cgtcttccca acgattggtg tagagcgggt agaagcggcc gctgtcggga
      961 tagatgccgt catactcggc cgtctcatcc agcttggcca acagcagcgt gcggccgagc
     1021 ggcgggttga gcgcggccat tagcaagccg tggtagcgcc gggtcagcac gttggcgatg
     1081 gtgccggaag caaagccgcc gatgccgtta gtgaccagcc actcgcgccg ggttgccgtc
     1141 gggagatcgc cgcacacttc gcggccgaaa tcgaatttca tggactactc cgtttgcgag
     1201 gacggcgtga tgggacttgg atcgtagccg acctgcaccg gccaatacta tagccacaaa
     1261 cggaacaagc gcacagtcga agccatgacg cgctactgct ttggcaagac ctgttaatgt
     1321 cgtttgttag ttccaccggc cagatataga atatttacac tgtttgtccc atttctgttt
     1381 ctgcagcgac attggggggc cgaatcatga tccgtttttt cctttgcatt ggtatcgcgt
     1441 tattcattgg gacaggttgc cgacaaacac ccgccgctgt ggagattgtc cnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatcggcca cccctgctcc taccagcatc
     1621 cggacagacc tcgatgcggc cgagcggatt gtgaccgatg gcggtttcgc ctttcgcttg
     1681 tttttgcccg acctggatgt ggagttgcaa cctactcagg ctttatttgc cgacaagaaa
     1741 ggtacgtttt tatcttcaat gggcggcggg gtggaggcgg atgacagcga ttcgctcgac
     1801 ataattctgt cggacatatt acagtctctt aacgaatcaa tggccgccgg tcttcaggcc
     1861 ggtgaacctt acccgatcac cgtcggtggc gttgaaggat tggctgtgga tgtttccggt
     1921 acgatgttcg atgggcctgt gctcggccga cttttggccg tccgcccata caaaggtcaa
     1981 attttctatg ccttcgctct cggtaatacc gcgtccggtg atgaacgttg ggttgaagac
     2041 ggcagcagct attttgaaac gttgctcgat tcgttgcgct ttttccccat tgcgctcggc
     2101 ggcgaagact cattaacgcc ggcccctgct gccggatcag atggcggccc ctgcccggtc
     2161 tccgtagacg aaacttatgg ctacacccag gaaaacgctg tccgggtcgg cggcggagca
     2221 ttcggcgggc cttcgcgcgc gattgcatac ctcgataccc tgcaagggcc tgccggggaa
     2281 gctgttactt atcatcggat cgggtccatg ccttttgaag atacgatttt ggatagctat
     2341 gaagtcagct acgaaggctc tgctcaaccc gccgtcctct ttgtagacca atacgccttc
     2401 gagacactct acgcgccggt cggttttact tgcaagatcc cattcccgct gacagcgccg
     2461 taatgcacct gattgttggt cattcatcga ttcctatgta ggatcgaggc atgacaaagt
     2521 cactcgttac cggccatcag ggcgaaacct cggaacacgt cacccagata atcgctcgcc
     2581 tgcgggccac ctatcccgac gcccattgcg agctggatta tgagacgccg atccagctgc
     2641 tggtcgccac catcctctcg gcccaatgca ccgatgcgcg ggtcaacatg accacgccgg
     2701 cgctgttcga acgtttcccc acggtcgaga cattggccgg agccgacccg gtggagatgg
     2761 aggagctggt gcggccgacc ggcttctatc gccagaaggc gcgctacatc cgcgaggcgg
     2821 cgacgcgaat tctgaccgag ttcggcggcg aggtgccgtc cgatctggat gatttgctga
     2881 cgctgcccgg cgtggcccgc aagacggcca acgtcgtgcg cggcgagatc ttcgggctgt
     2941 ccgacggcat caccgtcgat acccacgtga agcggctggc ggcgcggctg ggtctgacga
     3001 cggcgaccga cccggtcaag gtcgagcgcg acctgatggc gatcatcccg cgcgagagtt
     3061 ggcgagagat ctcgcatcta cttatctggc acggccgccg gctctgcacg gcgcgcaagc
     3121 ccgcctgcca tgagtgcccg ctgaacgaca tttgcccctc ggcgtttaag cctgcggatt
     3181 atcgaccggg aaagaacgac tgacgaacga caaacgatgg gtccgggaga ggagcgttat
     3241 cttataggta tttttactga ttttgcctct tgacgaagag aggtggggcg cgtagagtat
     3301 taccatagca agcagcctta tcgggcgctt gttttgagcc ggtttttggt caatccagtc
     3361 gctggggagc ttcgccgatg gacgatgcaa tccacgttag ccagtgcgca aagcgtgcgc
     3421 gtatgagcgc gctcttgata gttggtgggg ggaaggctca tgggcaccat actctctgat
     3481 ggtcctgttt cctattatca gcaactctat accatcctgc gcgatgaact gacccgcggc
     3541 aagtggaagc cgggggaacg catgccgtcg gagacggaac tcattgccgc ctacggtgtg
     3601 agccggatca ccgtgcggca ggcgttcgat ctgctggtca acgacgggtt cgtctatcgc
     3661 cggcgcggca gcggcacgtt cgtcaccgcg cggtcgagcg caccgagcct caatcgaatc
     3721 ctcagcttca ccgaggacat ggagcagcgc gggctgcgcc ccgagacgcg gccgatcgcc
     3781 gcgcgactgg aaccggccgc ggccgacgtg gcgcggaagc tgggcattga gccgggcgag
     3841 gagctggtcg tgctggagag tttgtgtctg gccgacgacg cgccgatgag cgtggagcgc
     3901 tcccatctgg tgcatcgcca ctgtcccggc ctgctggatg gggactatgc ccgcgtctcg
     3961 ctgcacagga cgctgctgga acggtacggc atccggcttg cccgcgccaa tcaggcggtt
     4021 tgggcggtag cggccggcgc ggaaacggcc gctgccctgt ccgtgctccc cggcgcgccg
     4081 ctgctctgca tcgagcaggt cctctttcgg cagaccggcg cccccgtcga gtatctccag
     4141 gcttaccggc gtggtgatcg gtatgtgctt tataacgagt tgcgggcttg atcaggggtc
     4201 tcgtcccgat caacgaccgg attctggagc gagccgatca ctcctaat
//
LOCUS       DAOMQG010000304         4247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8667_length_4247_cov_9.891937, whole genome shotgun sequence.
ACCESSION   DAOMQG010000304 DAOMQG010000000
VERSION     DAOMQG010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_4247_cov_9.891937"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..452)
                     /locus_tag="PLC06_13455"
     CDS             complement(261..452)
                     /locus_tag="PLC06_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91499.1"
                     /translation="MRRIIPILILLFTLAAAAPAAFAAEGDPLANSVLLHGMVGWAMV
                     GLALLLMALFILRTRRGPN"
     gene            complement(634..2505)
                     /locus_tag="PLC06_13460"
     CDS             complement(634..2505)
                     /locus_tag="PLC06_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91500.1"
                     /translation="MKENKSADALPVFKGTWALIRYRPWYFVVNLVFGVSFMVMGLLP
                     GLVTRRFFDELTGASPAAFGITTLLGLLVAVHLGRMVADVLTEWGGWSVRSVNSVLLR
                     VNFMRNILHKPAARPMPVESGDAIHRLERDVGDYADFPTWIPQEIGEALFFIFAVIIM
                     ARVNPWITLVAVLPLMTVFFINRIAWNRFLHYDRATRLSSSRVTGFLGEILGGVQAIK
                     VADAENGALHYFNTLSEARRKAGVRQSTFLTLVSSAGESMGAIAVAVVVLMAGQSLQS
                     GAMTVGDFTLFVTYLTLAVEFPANLGSYMSEIAQQRVVLDRLQEMHPDAPPESIVAHR
                     PIYEKGGAPAPALPIKTAADRLERLEVVGLSYRHREQGGEITNYELRITNEERSELVT
                     RHSPLVTGIADISFTLPRGSFTVVTGRVGSGKTTLLRALLGLLPPDSGEVCWNGVAVI
                     DPGVFFIPPRSAYTPQVPRLFSESLRDNILLGMPEDEADLTGALRAAVLEPDIAMLEA
                     GLDTVVGPRGVRLSGGQVQRAAAARMLVSNAELLVFDDLSSALDVETEQLLWERLVDE
                     AGEARPTCLVVSHRRPALRRADHIIVLEGGHIAAQGALDELLESSPEMRWLWEGKAG"
     gene            complement(2502..>4247)
                     /locus_tag="PLC06_13465"
     CDS             complement(2502..>4247)
                     /locus_tag="PLC06_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91501.1"
                     /translation="LFFAAVLAQNALNLLTVYLTEDLAWATTNALRADLTAHVLRLDM
                     GFHKLRTPGELIERIDGDVGQLADYFSEIVVNLIGNGLLVAGVIILIFLEDWRIGLLA
                     LSYAVVMITLMRAVQERMVRLHTRISQASAALLGFLEEHITGTEDVVPNGGANYVMAR
                     LYPLLNTLAALRTRTYTLSSIISATSTMLFALVLAGSMGLAALSYRAGAMTIGTVFAL
                     IYYVGLLQWPLNSVRRHLSDIQRALAKINRTREFFVLRPEVDDGAAAGTTALPPGAPG
                     VAFEGVSFAYKDRRGITNYELRITNEEGMVSDSELVTPTVLRDVTFTVAPGRVLGVLG
                     RTGSGKTTLSRLLFRLYDVDEGAIRLDDGRLPGEITNDELRITNEEEEEEVGGRPPVV
                     DPTDIRAIPLAELRRHIGMVTQDVQLFAATVRDNLTLFSNHDPARPPIDDEAILGALE
                     TLGLNDWYRGLPNGLDTVLESGGKGLSAGEAQLLAFTRVFLRDPRLVVLDEASSRLDP
                     GTEQLLERAIDRLLAERTGIIIAHRLRTIMRADDILILEDGRVIEHGPRSALAADPAS
                     RFYRLLQTGLEEVLA"
BASE COUNT          853 a         1346 c         1352 g          696 t
ORIGIN      
        1 aacatacttt tcaaaacaaa ggcgctctcc cttcgcaggg tcggcgccgt gtcccccggg
       61 cgggtcgcaa cacgcgacag gattcgcccg tttgttctag ggaatagtag cacgcttttc
      121 tgaaatcagt ctaatttctg agaaaaggtt aaaggaaagt attaatcgag gcggagaggg
      181 tggaaacagg caatcagcgc ccggcaccgc cgggcactga tcatcgtcga ccaaaagctg
      241 cggatcgact ggaagtggga ttagttcggc ccgcggcggg tccgaaggat gaacagagcc
      301 atcagcaaga gagccaggcc gaccatggcc caaccgacca tgccatggag caggacggag
      361 ttggccagcg gatcgccttc ggccgcaaaa gcggccggcg cggcggcggc cagagtaaac
      421 agcaggatta gaattggaat gattcggcgc ataggagttc ctctcgattt aggtcaagta
      481 cacgcaaact tgcgttgctg agcatcagaa gcatttcagt catggcctca tcaatcggct
      541 catgagagag taccggattg taacgcaatc caatgaagaa tggcgaacgg aagagcagat
      601 accgaagacg cgccggttag cgcgtcgctc cgtctacccg gctttccctt cccacagcca
      661 ccgcatctcc ggcgaacttt ccaacagctc atccagcgcc ccttgcgcgg cgatgtggcc
      721 gccttccagc acaatgatgt gatcggcgcg gcgcagcgcc gggcgacgat ggctgacgac
      781 gaggcaagtc ggccgcgcct cgcccgcttc gtccaccagc cgctcccaca gcagttgctc
      841 cgtttccacg tccagcgcgc tggagagatc gtcgaagacc agcagctcgg cgttactcac
      901 cagcatccgc gccgcggccg cacgctgcac ctgaccgccg gagagccgca cgccgcgcgg
      961 ccccacgacc gtgtccagcc ccgcctccag catggcgata tcgggttcca gtacggccgc
     1021 ccgcagcgct ccggtcagat cggcctcgtc ttccggcatg cccaacagga tgttatcgcg
     1081 cagggattcg ctgaacaacc gcggcacctg gggcgtatag gccgagcgcg gcgggatgaa
     1141 gaacacgccc ggatcaatta ccgccacacc gttccagcat acctcgccgc tatcgggcgg
     1201 cagcagcccc agcagcgccc gcagcagcgt cgttttgccg ctgcccaccc ggccggtgac
     1261 gacggtgaag gagccgcggg gaagggtgaa ggagatgtcg gcaataccag tgacgagtgg
     1321 cgagtggcga gtgacaagtt cagagcgctc ctcattcgta attcgtaatt cgtaattcgt
     1381 aatttcgccc ccctgctccc tgtgccgata cgacagtccc accacctcca gccgctccag
     1441 ccgatcggcg gccgtcttga tgggcagagc cggggccggg gcgccgccct tctcgtagat
     1501 ggggcggtgg gcgacaatgc tctccggcgg tgcgtcggga tgcatctcct gcaatcggtc
     1561 gagcaccacc cgctgctggg cgatctccga catgtagctg cccaggttag ccggaaattc
     1621 gacggctaga gtcagatagg tgacgaacag agtgaaatcg ccgacggtca tcgcgccgga
     1681 ctggagcgac tggccggcca tcagaacgac cacggccacg gcgatagcgc ccatgctctc
     1741 tccggcggag gagaccagcg tgaggaaggt gctctggcgc acgccggcct tgcgtcgcgc
     1801 ctcgctcagc gtgttgaagt agtgcagcgc gccgttctcg gcgtcggcca ccttgatcgc
     1861 ctgcacaccg ccgaggatct cgcccaggaa gccggtgacg cgactggagg aaaggcgggt
     1921 cgcccggtca taatgcagga agcgattcca ggcgatgcga ttgatgaaaa agacggtcat
     1981 tagcggcagc acggccacca gcgtgatcca tggattaacg cgagccatga tgatgacggc
     2041 gaaaatgaag aagagcgcct cgccgatctc ttgcgggatc cacgtgggga agtcggcata
     2101 gtcgcccacg tcgcgctcca gccgatggat ggcgtcgccc gattcgaccg gcatcggccg
     2161 cgcggcgggc ttgtgcaata tgttgcgcat gaagttcacc cgcagcaaca cgctgttgac
     2221 gctgcgcacc gaccaaccgc cccattcggt gagaacgtcg gcaaccatgc ggccaagatg
     2281 aacggccacc agcaggccca ataatgtggt aatgccgaag gcggccgggg aagcgccggt
     2341 cagctcgtcg aagaagcgcc gggtgacgag gccggggagc aaacccatca ccatgaaaga
     2401 gacgccgaac accaggttga cgacgaaata ccacggccga tagcggatca gcgcccaggt
     2461 acctttgaag acggggaggg catccgcgga tttattctct ttcatgccag cacctcctcc
     2521 aaccccgtct gcagcagccg gtagaagcgc gaggcggggt cggcggccag ggcagagcgg
     2581 gggccgtgct cgatcacgcg gccgtcctcc agaatgagga tgtcgtcggc gcgcatgatg
     2641 gtgcgcaggc gatgggcgat gatgatgccg gtgcgctctg ccagcagccg gtcgatggcc
     2701 cgctccagca gctgctctgt gccggggtcg agccgcgacg aggcttcgtc gagcaccacc
     2761 agccgcgggt cgcgcaggaa gacgcgcgtg aaggccaaca gttgggcctc tccggccgac
     2821 agccccttgc cgccgctctc cagcaccgtg tccagcccgt tcggcaggcc gcgataccag
     2881 tcattcaggc cgagcgtctc cagcgcgccg aggatagcct cgtcgtcgat tggcggccgg
     2941 gccgggtcat ggttgctgaa cagcgtcaga ttgtcacgca cggtggcggc gaagagctgc
     3001 acgtcctggg tcaccatgcc gatatggcgg cgcagttcgg cgagggggat agcgcggatg
     3061 tcggtgggat cgaccacggg cggccggccg ccgacctctt cctcttcctc ttcattcgta
     3121 attcgtaatt cgtcattcgt aatttctccc ggcagtcggc cgtcgtcgag ccgaatcgcc
     3181 ccctcatcca catcatacag ccggaacagc agccgcgaca gcgtcgtctt gccgctgccc
     3241 gttcgcccca gcacgcccag cacccggccg ggagcaacag taaacgtgac gtcacggaga
     3301 acggttggag tgacgagttc agaatcgctc accatcccct cttcattcgt aattcgtaat
     3361 tcgtaattcg taattcctcg ccgatccttg tacgcaaacg acacaccctc aaacgccacc
     3421 cctggcgcgc cgggcggcag ggcggtcgtg cccgcggccg cgccatcatc gacctccggc
     3481 cgcagaacga agaactcccg tgtgcggtta atcttggcca gcgcgcgctg aatgtcgctg
     3541 agatggcggc gcaccgaatt gagcggccat tgcagcagcc cgacgtagta gatgagcgcg
     3601 aacaccgtgc cgatcgtcat ggctccggcg cggtaggaca gcgccgccag ccccattgaa
     3661 ccggccagaa ccaatgcaaa gagcatggtg ctcgtcgcgc tgatgatgga gctgagggta
     3721 taagtgcgcg tgcgcagcgc ggcaagggtg ttcagcagcg ggtagagccg ggccatcaca
     3781 tagttcgcgc cgccgttggg cacgacgtcc tccgtgccgg tgatatgctc ctcgaggaag
     3841 cccaacagcg cggccgaagc ctgactgatg cgggtgtgga gccgcaccat gcgttcctgc
     3901 acggcgcgca tgagcgtgat catgacgacg gcgtagctca atgccaggag gccgatgcgc
     3961 caatcctcca gaaagataag gataatgacg cccgccacca gcagcccatt gccgatgagg
     4021 ttgacgacga tctccgaaaa gtagtcggcc aattggccca cgtcgccgtc gatgcgctcg
     4081 atcagctcgc caggcgtgcg cagcttgtgg aagcccatat cgagccgcag cacgtgggcg
     4141 gtcaggtcgg cgcgcagggc gttggttgtg gcccaggcca ggtcttcggt cagatagacc
     4201 gtgagcagat tgagcgcatt ctgggccagc acggcggcga agaacag
//
LOCUS       DAOMQG010000305         4232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8702_length_4232_cov_11.737850, whole genome shotgun sequence.
ACCESSION   DAOMQG010000305 DAOMQG010000000
VERSION     DAOMQG010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8702_length_4232_cov_11.737850"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="PLC06_13470"
     CDS             complement(<1..1778)
                     /locus_tag="PLC06_13470"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRO91502.1"
                     /translation="MRHNRLILILILILLLAACREQPQATPPQTTAEIAPTNEPAPTA
                     PPTATAAPQPTAAPTNVPLADLCVEPIYLAIIWHQHQPVYYKDPETGIYIKPWVRLHA
                     AKDYVDMAAILQEYPDIKATFNLTPSLIRQLDDLSAGATDLYWQHTIIPADELTDEQK
                     QFILDHFFDTNRKIIARFPRYQELLDLRDGSANPLVDFTTDDYRDLQLLFNLAWTDPD
                     WLAEGPPAVLVEQGEGFSEADKQVVLDEHLRLINEVVPIHREMQDSGQIEVTMTPFAH
                     PILPLLVDTDLALRATPDITLPNQPFRWGQDATAQIERGVQYYTDHFGRPPRGMWPSE
                     GSVAQEMVSMTAANGIQWMASSEGVLAHTLGMDSFTRDGNEVVVEADQLYRPYVVQGR
                     NGGPVAMVFRDIIISDKVGFTYSGVSGEAAADDFIDRIHNICGRLQSPDANGETPQGP
                     HLVSVILDGENAWEHYDNDGKDFLHALYQRLSDDPTIITVTPSEFLEMAPEPPRLRNL
                     WAGSWINSDFSTWIGEEEENRAWDLLQDTRVYLQTYLTGRNRENATPEQIAEAFTQMY
                     IAEGSDWFWWYGSDQTSGDDGSFDQQ"
     gene            complement(2016..2927)
                     /locus_tag="PLC06_13475"
     CDS             complement(2016..2927)
                     /locus_tag="PLC06_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRO91503.1"
                     /translation="MTASTETTGRGAGSSLSAWRQSKKARFRIILAFKYLIAAIVLVY
                     SLFPILWTISASFSPSGSISGQTLIPSPPTLRNYERILDQDFWLWMRNSFIISSISAL
                     LVGLITLMSAYAFSRFRWKGRSQLLLMILLIQVFPAILAMVALFAILQQIGNYIPFLG
                     LNTHGGLILIYMGGALGVNVWLMKGFFDSIPREIDESGKVDGASDWQIFWQLLFPLVR
                     PIMVVSMILTFFGTYSDYLMPRVMITTASKFTLMLGLQTFIGTNYSQEWGAFAAGAVM
                     GAIPMVTVYLLLQDYIVGGLTAGSVKG"
     gene            complement(2927..>4232)
                     /locus_tag="PLC06_13480"
     CDS             complement(2927..>4232)
                     /locus_tag="PLC06_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRO91504.1"
                     /translation="ISFTNFGAANLLPKVQAISVLEQRTFLPETGATLQYTVFQNDET
                     GEYGLWLIHPDGHSFFATTTDELEAEAIGAGPLNADGVPTAIPGWTQLTRAQTVRNIT
                     AISQTTFGREETAVSLTGRLGQAAQLQPRFIHDAQADTITDKRDNIVYTANNETGFFT
                     APDGTKLSPGFQVVIGMQNYKRFLTDPSFREPLLRIFIWNVVFALLSVLLSFALGLMI
                     AVVFGRTMPGQRIIKSLLVIPFAVPQVITLLVWRGILNPLEGVVPNTLQSIFNQPVGW
                     PPFFADPIWVKVALIIINVWLAYPYFMLISSGALQAIPTDMYEAADIDGASAWQQFRG
                     LTLPLLLVTVGPLLISSFIVNFNSFNVIYLFNSGGPPMVGTALPAGHSDILISYVYNL
                     AFGRGLALYGYASAITIMIFFMMVIVTIFQHRRMAAWEERTM"
BASE COUNT          835 a         1158 c         1425 g          814 t
ORIGIN      
        1 aactgctggt cgaatgagcc gtcgtcgccc gacgtctggt cggagccata ccaccagaac
       61 cagtcggagc cttcggcgat atacatctgg gtgaaggcct cggcgatctg ttccggggtc
      121 gcgttctcgc ggttgcggcc ggtgaggtag gtttgcagat agacgcgggt gtcctggagc
      181 aagtcccagg cgcggttctc ctcttcctcg ccgatccatg tggagaaatc gctgttgatc
      241 cacgatccgg cccacagatt gcgcaggcgc ggcggctcgg gggccatttc cagaaactcc
      301 gacggcgtca cggtgatgat ggtggggtcg tcgctcagtc gctggtagag cgcgtggaga
      361 aaatctttgc cgtcgttgtc gtaatgctcc caggcgttct cgccgtcgag gatgaccgac
      421 acaaggtgcg gcccctgggg cgtctcgccg ttggcgtcgg gcgattgcag gcggccgcag
      481 atattgtgga tgcggtcgat gaagtcgtcg gccgccgcct cgcccgatac gccggaatag
      541 gtgaagccga ccttgtcgga aataatgata tcgcgaaaca ccatggccac ggggccgccg
      601 ttccgcccct gcacgacgta aggtcgatag agctggtcgg cttccaccac gacctcgttc
      661 ccgtcgcgag tgaagctgtc catgcccagg gtgtgggcga gcacgccctc gctgctggcc
      721 atccattgaa tgccgttggc ggccgtcatg ctgaccattt cctgggcgac ggagccttcg
      781 ctcggccaca tgccgcgcgg cggtcggccg aaatggtcgg tgtaatattg cacgccgcgc
      841 tcgatctgtg ccgtggcgtc ctgcccccaa cggaagggct ggttgggcag ggtgatgtcg
      901 ggcgtcgccc gcagcgccag atcggtatcc acgagcagcg gcaggatggg atgggcgaac
      961 ggcgtcatcg tcacttcaat ctggccgctg tcctgcatct cccggtggat ggggacgacc
     1021 tcgttgatga gccgcagatg ctcgtcgaga acgacctgct tgtcggcctc gctgaagcct
     1081 tcgccctgct cgaccagcac cgccggcggg ccttcggcca gccagtcggg gtcggtccag
     1141 gccagattga agagcagttg caggtcgcgg taatcatcgg tcgtgaagtc gaccaacggg
     1201 ttggcgctgc cgtcgcgcag atccagcagt tcctgataac gcgggaagcg ggcgatgatc
     1261 ttgcggttgg tatcgaagaa atgatcgagg atgaactgct tctgctcgtc ggtcagttcg
     1321 tcggccggga tgatcgtatg ttgccagtag agatcggtcg ccccggcgct gaggtcgtcg
     1381 agctgccgga tgaggctggg cgtcaggttg aacgtggcct tgatgtcggg atattcctgc
     1441 aggatagcgg ccatatcgac gtagtccttg gcggcgtgga ggcgtaccca gggcttgatg
     1501 tagatacctg tctcggggtc cttgtaatag accggctggt gctgatgcca gatgatggcc
     1561 agatagatcg gctcgacgca caggtcggcc agcggcacgt tggtcggcgc ggccgtgggc
     1621 tggggggcgg cggtggccgt cggcggggct gtgggggccg gttcgttggt tggcgcgatt
     1681 tcggccgtcg tctggggcgg cgtggcctgc ggttgctcgc ggcaggcggc aagcagcaga
     1741 ataaggataa ggatgagaat cagtcggttg tgacgcatag cgttccctca tttactcgta
     1801 gcaaggcgcg accgtcaatg ggctgaagct cgccggaacc ggctacaaga caaaactatc
     1861 tttttaacag tatgatcaag aatcttgtgt atcaccccgc cttgtctcaa gcaaaagggg
     1921 tataccgtag tatacccccc tgccataaac atgtggaaac cggatttggc gaacagaaat
     1981 tcgctcagaa taacccggtt cccgatttca caatattacc ccttcacgga cccggccgtc
     2041 agacccccaa cgatataatc ctgcagcagc aggtacacgg tgaccatcgg gatggctccc
     2101 atgaccgcgc cggcggcgaa cgccccccat tcctgggagt agtttgtacc gatgaacgtc
     2161 tgcaagccga gcatgagcgt gaacttgctg gccgtggtga tcatgacgcg cggcatcagg
     2221 tagtcgctgt aggtgccgaa gaaggtcagg atcatcgaca cgaccatgat cggccgcacc
     2281 agcgggaaca gcagctgcca gaagatttgc cagtccgagg ctccgtcgac tttgcccgat
     2341 tcatcgatct cgcgcgggat ggagtcgaaa aagcccttca tcagccagac gttgacgccc
     2401 agcgcgccgc ccatgtagat caggatcaac ccgccgtggg tgttcaggcc cagaaacggg
     2461 atgtagttgc cgatctgctg caggatggcg aagagggcaa ccatggccag gatggccggg
     2521 aacacctgga tgagcaggat catcagcagg agctggctgc gccccttcca gcggaagcgc
     2581 gagaaggcat aggccgacat cagggtgatg aggccaacca gcaaggccga gatggacgag
     2641 atgatgaacg agttgcgcat ccacagccag aagtcctgat cgaggattcg ctcgtagttg
     2701 cgcagcgtgg gcggcgacgg gatgagcgtc tgacccgata tgctgccgct ggggctgaac
     2761 gaagccgaga tcgtccacag gatcgggaac agggaataga ccaggacgat ggcggcgatg
     2821 agatacttga acgccagaat aatgcggaag cgggccttct tgctttgacg ccaggcgctg
     2881 agggatgaac ccgcaccgcg gccggtggtt tcggttgaag cggtcattac attgttctct
     2941 cctcccaggc agccatgcgg cggtgctgga atatcgtcac gatgaccatc atgaagaaga
     3001 tcatgatcgt gatggcggat gcgtagccat agagggccaa cccgcgcccg aaggccaggt
     3061 tatagacgta gctgatcaga atgtcggaat gcccggcggg caatgccgtt ccgaccatgg
     3121 gcgggccacc gctgttgaac aggtagatga cgttgaagct gttgaagttg acgatgaaag
     3181 acgagatgag cagcgggccg accgtcacca gcaacaacgg cagggtgagg ccgcggaact
     3241 gttgccaggc gctggccccg tcgatgtcgg ccgcctcata catatccgtc gggatagcct
     3301 gcagcgcgcc gctgctaatg agcatgaaat acggataggc cagccagacg ttgatgatga
     3361 tcaacgccac cttgacccag atggggtcgg caaaaaacgg cggccatccg accggttgat
     3421 tgaagatgct ctgtagcgta ttggggacga caccttccag cgggttcagg atgccgcgcc
     3481 agaccaggag cgtgatgacc tggggcacgg cgaagggtat gaccagcagc gatttgatga
     3541 ttcgctggcc gggcatcgtc cggccgaaga cgacggcgat catcagcccc agcgcgaatg
     3601 aaagcaaaac gctgagaagg gcgaagacga cgttccagat gaaaatgcgc agcaacggct
     3661 cgcgaaacga cgggtcggtc agaaaacgtt tgtagttttg catgccgatg acgacctgga
     3721 agcctgggct gagtttggtg ccgtctggag cggtgaaaaa gccggtctcg ttgttggccg
     3781 tataaactat attgtcgcgc ttgtcggtga tcgtgtcggc ttgtgcatcg tggatgaagc
     3841 gcggctggag ctgggcggcc tggccgaggc gaccggtgag agacacggcc gtctcttcgc
     3901 ggccgaatgt cgtctggctg atggcggtga tgttgcgcac tgtttgggcc cgcgtcagtt
     3961 gcgtccagcc ggggatggct gtcgggacac cgtcggcgtt tagtgggccg gcgccgatag
     4021 cttcggcttc cagttcgtcg gtcgtggtgg cgaaaaagga gtgaccgtcg ggatggatga
     4081 gccacaagcc atattcaccg gtctcgtcgt tctggaagac ggtgtattgc agcgtggctc
     4141 ccgtctccgg caaaaaggtg cgctgttcca gcaccgagat ggcctgcacc ttgggcaaga
     4201 ggttggccgc gccgaagttg gtgaacgaga tg
//
LOCUS       DAOMQG010000306         4231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8705_length_4231_cov_6.834531, whole genome shotgun sequence.
ACCESSION   DAOMQG010000306 DAOMQG010000000
VERSION     DAOMQG010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8705_length_4231_cov_6.834531"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..971
                     /locus_tag="PLC06_13485"
     CDS             93..971
                     /locus_tag="PLC06_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO91505.1"
                     /translation="MKSITRLLAILLLPLLLLFAAGVAHADTTHVVQPGDTLWEIAMQ
                     YGVSMDAIVEANNLENPRVIRLGSTLTIPGVDGPLMSLPMPAPGGTAPAAPDSAEAPV
                     NLFKNGSFEGDWYYHIYNELQIPVGWQVAIDEGPNTLQPGDGGNFARPEIRVVTRASL
                     AEGEGDLFVFDGYKTMKAFKGYAPTIFHFFQDVPLQPGRYRMTLNFFPDIVASYTGGR
                     QFHTDPLAGEIRIIHNEDNLGWTSVKPGEKNWRIYEFTVSQAGTVRLGASFRNRFAAA
                     NNGWFLDDWRLERIGK"
     gene            complement(1111..1776)
                     /locus_tag="PLC06_13490"
     CDS             complement(1111..1776)
                     /locus_tag="PLC06_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRO91506.1"
                     /translation="MCGRFALDATGEEVAEHYQLSEVPFIVPRYNIAPTQPVAAVRLN
                     RAGERELTFFRWGLIPSWAKDPSIGSRLINARAETAAEKSSFRAAFKRRRCLLPATGF
                     FEWRKVGGGKQPMYIHETDEGIMSLAGLWEIWQSPDGGELETCTILTTEPNALMAPIH
                     DRMPVILDPLDYDMWLAADTPADQLQHLLRPFDAGQLAAWPVSTAVNKPQNDTADVIA
                     PIG"
     gene            complement(1811..3421)
                     /locus_tag="PLC06_13495"
     CDS             complement(1811..3421)
                     /locus_tag="PLC06_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRO91507.1"
                     /translation="MSTGQALLFIVVGGGVALIVAAIIAFLVSRSVRNSTKRQLESQL
                     KAQKDLNDEQVRALRGALSEGQQQQKELLERLAAERPAEPVAPPPAPTAVIAQASTPA
                     PNENGPGQESAFLRERLVKEKYAQGANLRKAFLRGLDLSSADLRDANLKQANLQEADL
                     SGADLRGADLSGADLKRALLVGAQLSGADLSGANLGRAVVNGADLIGADLNQADLSGM
                     LMDEETRLDIKWQTVWEIVNRGAAGRDLSQADLRDADLWGADLRAARLWETDLSAARL
                     TRADLRRAKLWRAKIDDRTQIDEKWRLTWGIVNQGAAGRDLREIDLSESDLSRADLSR
                     AALTKANLSQTDLSHADLSGANLSGANLSRSSLVATDAREANLSGANLNGADLSKADF
                     SRAKMSWTDLRNTVVSELTQIDPKWRLVWEIVNQGAMQRDLSRADLAGANLREANLNG
                     ADLRGAKLWLADLAGADLRRAGLRNTDLRESNLVGADLSDTNLQDANLTGARFDGTTR
                     WPAGFDPMTVGALPRSAGRVERQPEPVD"
     gene            complement(3597..3809)
                     /locus_tag="PLC06_13500"
     CDS             complement(3597..3809)
                     /locus_tag="PLC06_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91508.1"
                     /translation="MKTAVSSNGKPLVAAPGAPAQAICPKCGGLVTLRVRKLMANSGT
                     SSYWRHEDDDHPCQRQKPFMRVRPAM"
     gene            complement(4137..>4231)
                     /locus_tag="PLC06_13505"
     CDS             complement(4137..>4231)
                     /locus_tag="PLC06_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRO91509.1"
                     /translation="VREEVRHTVIHEIAHHFGIDDDRLVELGAY"
BASE COUNT          761 a         1313 c         1356 g          801 t
ORIGIN      
        1 atgagcggga gtttgcctaa tttggccgta tggagataat tccggcggga gcttttcgag
       61 catctcaaat caatacagga catgactaca gtatgaaatc gataactcgt ttgttagcta
      121 ttttgttgct gccgctcttg ctgctgtttg ctgcgggcgt ggcccatgcg gacacgaccc
      181 acgtcgtgca gccgggtgat acgctgtggg agatcgccat gcagtacggc gtttcgatgg
      241 atgccatcgt tgaggccaac aacctcgaaa acccgcgcgt gatccgcctg ggatccacat
      301 tgaccatccc cggcgtcgac ggcccgctca tgagcctgcc gatgcccgcg ccgggcggca
      361 ctgctccggc cgcgcccgat tcggccgagg cgccggtcaa tctgttcaaa aacggcagct
      421 tcgagggcga ctggtattac catatttaca acgagctgca aatccccgtc ggctggcaag
      481 tggccatcga cgaaggcccc aacacgctcc agcccggcga cggcggcaac ttcgcccggc
      541 cggaaatccg cgtcgtcacc cgcgcctcgc tggccgaggg cgaaggcgat ctgttcgtct
      601 tcgacggcta caagacgatg aaggcgttca agggctatgc gccgaccatc ttccacttct
      661 tccaggatgt gccgctgcag cccggccgct accggatgac gctcaacttc ttccccgaca
      721 tcgtagcgag ctacaccggc ggccgtcagt tccacaccga cccgctggcc ggcgagattc
      781 gcatcatcca caacgaggat aatctgggct ggaccagcgt gaagccggga gaaaagaact
      841 ggcgcatcta tgagttcacc gtcagccagg cgggcacagt gcgcctcggc gcgtcgttcc
      901 gcaaccgctt cgccgcggcc aacaacggct ggttcctcga cgactggcgc ctggagcgca
      961 tcgggaagta gattcaaggc tccggaacgt agtccgcggc cgcggactgc gcaaattgag
     1021 gcaacaggcc agtggccggc agtcagcccg gccgctggcc tgtatgatca ctgacccact
     1081 gaccactgat ctactgatca ctgacccaca tcacccgatc ggggcgatga cgtcggcggt
     1141 gtcgttctgc ggcttgttga cagccgtgct gaccggccag gccgcgagct gcccggcatc
     1201 gaaggggcgc agcaggtgct gcagctggtc ggcgggagtg tcggccgcca gccacatgtc
     1261 gtaatcgagc gggtcgagga tcaccggcat ccggtcgtgg atcggcgcca tcagcgcgtt
     1321 cggctcggta gtcaggatgg tgcaggtctc cagttcgccg ccgtcaggcg attgccaaat
     1381 ctcccacaag ccggccagcg acatgatacc ctcatccgtt tcatggatat acatgggctg
     1441 tttgccgcca cccaccttgc gccactcgaa gaagccggtg gccgggagca ggcagcgccg
     1501 ccgcttgaag gcggcgcgaa acgacgactt ctcggccgcc gtctcggcgc gggcgttgat
     1561 caaccgcgag ccgatggacg ggtccttggc ccatgacggg atcagccccc agcggaaaaa
     1621 ggtcaattcg cgctccccgg cccggttcag ccgcacggcg gcgacgggct gggtgggcgc
     1681 gatgttatag cgggggacga tgaagggcac ctcggacagc tgatagtgct ccgccacctc
     1741 ttcaccggta gcatcgaggg caaaacggcc gcacatatag ccaactcctc ggtccggcgt
     1801 tcggccggac ctaatcgacg ggttccggct ggcgctcgac gcggcccgcg ctgcggggga
     1861 gcgcgccgac cgtcatcggg tcaaaccccg ccggccagcg ggtggtgccg tcgaagcgcg
     1921 cgccggtcag gttcgcgtct tgcagattcg tgtcggacaa gtccgcgccg acgaggttgg
     1981 actcgcgcag gtcggtgttg cgcaggccgg cacggcgcaa gtccgctccg gccaggtcgg
     2041 ccagccagag cttggccccg cgcagatcgg cgccgttaag gttggcctcg cgcaggttgg
     2101 cgccggccag gtcggcccgg ctcaggtcgc gttgcatcgc gccctgattg acgatctccc
     2161 acaccagccg ccacttgggg tcgatttggg tcagttcgga gacgacggtg ttgcgcaggt
     2221 cggtccagct catcttggcc cggctgaagt cggccttgga cagatccgcg ccgttgaggt
     2281 tggcgccgct gaggttggcc tcgcgggcgt cggtggcgac gagagacgag cggctgagat
     2341 tggcgccgct gaggttggcg ccgctcaggt cggcatggct cagatcggtc tgcgacagat
     2401 tggccttggt cagagcggcg cggctgaggt cggcgcggct gaggtcgctc tccgacaggt
     2461 caatctcgcg caaatcgcgc ccggcggcgc cctgattgac gatgccccac gtcaggcgcc
     2521 atttctcatc gatctgggtg cgatcatcaa ttttggcccg ccatagcttg gcccggcgca
     2581 ggtcggcgcg ggtgaggcgc gcggccgaga ggtcggtttc ccacaaacgg gcggcgcgca
     2641 gatcggcccc ccagaggtcg gcgtcgcgca ggtcggcctg gctgaggtcg cgcccggccg
     2701 cgccgcgatt gacgatctcc cacacggtct gccatttgat gtcaagcctg gtctcctcat
     2761 ccatcagcat gcccgacagg tcggcctgat tgagatcggc gccgattagg tccgcgccat
     2821 tcaccaccgc gcggcccaga ttcgcgccgc tcagatcggc gccggacagt tgggcaccga
     2881 ccagcagcgc ccgcttgagg tcggcgccgg agaggtcggc gccgcgcagg tcggccccgg
     2941 agaggtcggc ttcctgcagg ttggcctgct tcaggttggc gtcgcgcagg tcggccgagg
     3001 agagatcaag cccacgcaga aaggccttgc gcagattggc gccctgggcg tacttctcct
     3061 tcacgaggcg ctcgcgcaga aaggccgatt cctggccggg gccgttttcg ttgggcgccg
     3121 gggtggaggc ctgggcgatg accgccgtcg gggcgggcgg cggcgcgaca ggttcggccg
     3181 ggcgctcggc ggccaatcgt tccagcagct ccttttgctg ctgctgcccc tcgctcagcg
     3241 ctcccctaag ggcgcgcacc tgctcatcgt tgaggtcctt ttgcgcctta agctgcgatt
     3301 caagctggcg cttggtggaa ttgcgaacgg accgggaaac aagaaaggcg atgatggcgg
     3361 ccacaatcag ggccacccca ccgcctacaa cgataaataa caatgcctgg cctgtgctca
     3421 tgagtgaagt ctccaaaagt cgatgcaggg cttaatgagg tctgctgcaa gccacgaacg
     3481 acaaacgatc gcggccgatc tgactagtct gttgcccatt ctaacatgat aagggcaggc
     3541 acggaaatat tcggttgtca aaattacggt cttgtcacgc ggcttttgcc caattttcac
     3601 atcgccgggc gcacccgcat gaacggcttc tgacgttggc agggatgatc gtcatcctca
     3661 tgccgccaat aactgctggt gccgctgttg gccatcaatt tgcgaacccg caacgtgacc
     3721 agcccgccac acttcggaca aattgcctgc gccggcgcgc cgggagcagc aacaagtggc
     3781 ttgccattgg acgatactgc tgtcttcatg gtctcatctc ctgttttggt gcgttggcgg
     3841 ccgaattggg ctgacgcaaa gcagctgacg aataaccatt acctcttgcc ggcggcaaaa
     3901 cgaaacagca acgtggcagc gactggagcc aaaattcagc gcaacgcttg aaataagcct
     3961 ttatttactt gttggtacta ctctgggtat tgatacctat acggtaagtg taatagtagg
     4021 acaacttgac gataaatgca agacataatc catgcaatct gccaaattgg tgtgttttct
     4081 cagaaattgg gggttttggg gcgttgtaat gtggattgta atgtttctgt tttatcttag
     4141 taagcgccca actcgacgag gcgatcgtca tcgataccga agtggtgggc gatctcgtgg
     4201 atgaccgtgt ggcgcacctc ctcgcgcacg c
//
LOCUS       DAOMQG010000307         4213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8742_length_4213_cov_9.749399, whole genome shotgun sequence.
ACCESSION   DAOMQG010000307 DAOMQG010000000
VERSION     DAOMQG010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_4213_cov_9.749399"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLC06_13510"
     CDS             <1..369
                     /locus_tag="PLC06_13510"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRO91510.1"
                     /translation="EHQLAVHPNCGTVLVTTAALATLAAQIALAVESRRGPKSGVGPL
                     AMLNAMPGAMVAVVGAIIVGRPLGVRLQERYTVDGDLRGLEVESVQRIAPSPITRLFQ
                     LLLTGGNPDLAAKAYFVRTR"
     gene            482..1468
                     /gene="holA"
                     /locus_tag="PLC06_13515"
     CDS             482..1468
                     /gene="holA"
                     /locus_tag="PLC06_13515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRO91511.1"
                     /translation="MFHLFHGQDTYSQRETLTALLAKEGDPDMLSLNTTRLDNRITFA
                     ELQSACDAMPFLSRVRVVLVQDLFSSIPDKAFMEKLEAYLPELPKATRLIFLESQPLS
                     DSHRIVRLAQEKKIGYVRKFDPPKGGDLERWVRIHVEERGGRIAPQAAVLLATNVAGE
                     RDERGNPLPSLAALTNEIEKLVLYKGSEATIEAADVALLSPYAAEDTIFELVDALGNR
                     QTARAAELFQDKINEGADPFYLFSMFIRQFRLLIQARYLLDQGERAAGIAEQMKQKSF
                     VAEKLARQARNFTLPQLEQIYRRLLEIDVDAKTGKADLMTSLYLLVAGLTVE"
     gene            1509..2213
                     /locus_tag="PLC06_13520"
     CDS             1509..2213
                     /locus_tag="PLC06_13520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRO91512.1"
                     /translation="MAHLQGQERADYVQDMFARVAERYDLMNRLMTFGQDLRWRRYVI
                     LKANLPAGGRLLDIATGTGDIADEGRRQVPGLRAVGGDFTIEMMQAGKRIPERRDIRW
                     TAADTLALPFPDDTFDAVTSGFLMRNVIDVAGAFREQVRVTKPGGRVVVLESSPPKDN
                     LLKPFILIHLNVIIPTLGRIVAGQSDAYHYLPDSTQAFQGPERLAEAMRAAGLVDVSY
                     RLFMFGTIGVHVGTKR"
     gene            2271..2903
                     /locus_tag="PLC06_13525"
     CDS             2271..2903
                     /locus_tag="PLC06_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO91513.1"
                     /translation="MTAIDLNGKVAIVTGAGQGAGKAIAEALVAAGARVAVNDINPDR
                     AYRVATELRERGGTTIGVMADVSNRFQCVHLIETTRNEWGRIDILINCAAIQPSASIL
                     KMDEWELMRVFDVNMKGPFFMSQLVGRVMSDRHKVDGARGLMITLAAAEPEQVGLAAY
                     AATQAALPAFVRACAREYAPLGIGVLLLPIAGKSEQTAAAAMALIAEQLG"
     gene            2987..3754
                     /locus_tag="PLC06_13530"
     CDS             2987..3754
                     /locus_tag="PLC06_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO91514.1"
                     /translation="MSLRQMLPFFLLNIIVSAVVVLSILFWWDNRGPGNGLAAATATA
                     ALPEGVLPTPNIAAPPSGTVPTEAESAASEPVVHIVQAGETLNIISQQYDVSVDDIMA
                     ANGITDPDVITVGQVLTIPVGGLPEPTVAPTETAVANPSPIPTEPAAVGGGVIGVTGV
                     IDPGVLETEAVQLVNSGAQEQSIDGWQIRDEDSNVYIFGGVSIFGEGAGVMLHTRAGD
                     DTYSDLHWGMTQPVWRSGEQLTLFDAGGQVVATFVVP"
     gene            3797..4021
                     /locus_tag="PLC06_13535"
     CDS             3797..4021
                     /locus_tag="PLC06_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91515.1"
                     /translation="MRGLISPKGEQFGYLIGDTLYTMDDEPSGRLDGDYIVDLAGNPV
                     WRVVGDGVYALNGMETIGFLSAERADERDL"
     gene            complement(4025..4213)
                     /locus_tag="PLC06_13540"
     CDS             complement(4025..4213)
                     /locus_tag="PLC06_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91516.1"
                     /translation="MLLKDATRGKYAARYEAGTNVVLLEPDVAAAFPTDQAVNDALRL
                     VLRLSRIPLPAEENAPIP"
BASE COUNT          788 a         1345 c         1311 g          769 t
ORIGIN      
        1 gagcatcaac tggccgtcca tcccaactgc ggcacggtgc tggtgacaac ggccgcattg
       61 gccacgctgg cggcccagat cgcgctggct gtcgagagcc ggcgcgggcc gaagagcggc
      121 gtcgggccat tggcgatgct gaacgccatg cccggagcca tggtcgccgt cgtgggcgcg
      181 atcatcgtcg ggcggccgct gggcgtgcga ctgcaagagc gttacacggt cgacggcgat
      241 ctgcgcggtc ttgaggtcga atccgttcag cgcattgccc cgtcgcccat cacccgcctg
      301 ttccagctgc tgctgaccgg cggcaatccc gatctggcgg ccaaggccta ttttgtgcgg
      361 acaagatgaa ttacgaatta cgaattacga attacgaacc aaccaccggc ctaccggcct
      421 actgacctac cgatctaccg acctactgat taccgaaccc attcgtaatt cgtaattcac
      481 catgtttcac ctcttccacg gccaggacac ctactcccag cgggagacgc tgaccgcgct
      541 gctggccaaa gagggcgacc cggatatgct gtcgctcaac acgacgcggc tggacaaccg
      601 gatcaccttc gccgagctgc aatcggcctg cgacgcgatg ccgtttctgt cgcgcgtgcg
      661 ggtggtgctg gtgcaagacc tcttttcgtc catccccgac aaggcgttca tggagaagct
      721 ggaagcctat ctgccggagc tgcccaaggc gacgcggctc atcttcctgg aatcgcaacc
      781 gctgtccgac agccatcgca tcgtgcggct ggcccaggag aaaaagatcg gctacgttcg
      841 aaagttcgac ccgcccaagg gcggcgatct ggaacgctgg gtgagaatcc atgtcgaaga
      901 gcgtggcggc cgtattgcgc cccaggcggc cgttctgctg gcgaccaacg tcgccgggga
      961 acgggacgag cgcggcaacc ccttgccgtc gctggcggcg ctgaccaacg agattgagaa
     1021 gctcgtcctc tataagggca gcgaggcgac aattgaggcg gccgacgtgg ccctgttgtc
     1081 gccctacgcg gccgaggaca cgatcttcga gctggtggac gcgctgggca accggcaaac
     1141 ggcccgcgcg gccgagctgt tccaggacaa gatcaacgaa ggggccgacc cgttttacct
     1201 gttctccatg ttcattcgcc aattccggtt gctgatccag gcgcgctatt tgcttgacca
     1261 gggggagcgg gcggcgggca tcgccgagca gatgaagcag aaatcgttcg tggccgagaa
     1321 gctggcccgc caggcgcgca acttcaccct gccgcaactg gagcagatct accgccgcct
     1381 gctggagatc gacgtcgacg ccaagaccgg caaggccgac ctgatgacct cgctgtacct
     1441 gttggtggcg gggctgactg tggaatagca cagtctgtca tggcacaaaa cctgaggatt
     1501 actcgattat ggcacacctg caaggacaag aacgcgccga ctacgtacag gacatgttcg
     1561 cccgtgtcgc cgagcgctat gacctgatga accggttgat gacctttggc caggatttgc
     1621 gctggcgacg ctacgtcatc ctcaaggcca atctgcctgc cggcggccgc ctgctcgaca
     1681 tcgccaccgg cacgggcgac atcgccgacg aggggcggcg gcaggtgccg gggctgcgcg
     1741 ccgtcggcgg cgacttcacc attgagatga tgcaggcggg caagcgcatc cccgaacggc
     1801 gcgacatccg ctggacggcg gccgacacct tggccctgcc cttccccgac gacaccttcg
     1861 acgccgtcac gtccggcttt ctcatgcgca acgtcatcga tgtggccggc gcgtttcgcg
     1921 agcaggtgcg cgtgacgaaa cccggcggcc gcgtcgtcgt cctcgaatcc agcccaccca
     1981 aggacaacct gctcaagccg ttcatcctga tccacctcaa cgtcatcatc cccacgcttg
     2041 gtcgcatcgt cgccgggcag agcgacgcct atcattatct gcccgacagc acccaggcct
     2101 ttcaggggcc ggaacgtctg gccgaagcga tgcgcgccgc cggtcttgtc gatgtgagct
     2161 accggctgtt catgtttggg acaattgggg tgcatgtagg caccaagcgg taggttattt
     2221 ccatcctgtt tacagtcgcc aactccccgg attgccggta tgatcccctc atgacagcaa
     2281 tcgatttaaa cggtaaagtc gccatcgtca ccggcgcggg gcagggcgcg ggcaaggcca
     2341 tcgccgaagc tctggtcgcg gccggggcgc gggtggccgt caacgacatc aaccccgatc
     2401 gcgcctatcg tgtggccacc gaactgcgcg agcgcggcgg cacgaccatc ggcgtaatgg
     2461 ccgatgtgag caatcgcttc caatgcgtcc atctgatcga gacgacccgc aacgaatggg
     2521 gtcgaatcga catcctcatc aactgcgccg ccatccagcc atccgcctca atcctgaaaa
     2581 tggatgagtg ggagttgatg cgcgtgttcg atgtgaacat gaaaggcccg ttcttcatga
     2641 gccagctcgt cggccgtgtc atgtccgatc gccataaggt cgatggcgcg cgcggactga
     2701 tgatcaccct ggccgccgcc gaaccggagc aggtgggtct ggccgcctat gccgccaccc
     2761 aggcggcgct gcctgctttc gtccgcgcct gcgctcgcga atacgcgccg ctgggcattg
     2821 gcgttctcct gttgcccatc gccggcaaat ccgagcaaac ggccgcggcc gccatggcgt
     2881 tgatcgcgga gcaacttggg tgaagaacgg tgcgcgccaa cctgggagaa agcctctccg
     2941 gtcactcgct gctgacaaac catccatgag gcggtaaaat caccccatgt ccttgcgcca
     3001 gatgctgccc ttttttctgt tgaatatcat cgtttcggcc gttgtggtgt tgtccatcct
     3061 gttttggtgg gacaatcgcg gtcccggcaa tgggcttgcg gccgcaaccg cgactgctgc
     3121 gctgccggaa ggcgtattgc ccacgcccaa catcgccgcg ccgccttcgg gcacggtccc
     3181 gacggaagcc gaatcggccg catccgagcc ggtggtccac atcgtccagg cgggcgaaac
     3241 gctcaacatc atcagccagc aatacgacgt cagcgtcgac gatatcatgg ccgccaacgg
     3301 cataaccgat cctgacgtta tcactgtggg gcaggtattg accatcccgg ttggtgggtt
     3361 gccggagccg actgtcgcac cgaccgaaac ggccgtggcc aaccccagcc ccatcccgac
     3421 cgaaccggcg gccgttggtg gtggcgtcat cggcgtgacc ggcgtcatcg accccggtgt
     3481 gctggagacc gaggccgtgc aactggtcaa cagcggcgcg caggagcaat cgatcgacgg
     3541 ctggcagatt cgcgacgaag acagcaacgt ctatatcttc ggcggcgtca gcatcttcgg
     3601 cgaaggggct ggggtgatgc tccacacccg ggccggtgac gacacctaca gcgacctgca
     3661 ttggggcatg acccagccgg tctggcgcag cggcgaacaa ttaaccctgt tcgacgcggg
     3721 cggtcaggtg gtggcgacgt ttgtcgtccc gtgatcggcc gcccgggcca ctcgccccaa
     3781 cccgtggagt tatctcatgc gtggattaat cagccccaaa ggggaacagt ttggctatct
     3841 gatcggcgac acgctctaca caatggatga tgagccaagc ggccggctgg acggggatta
     3901 tatcgtcgac ctggccggca acccggtgtg gcgcgtcgtc ggcgacggcg tgtatgcgct
     3961 gaacgggatg gagacgattg gttttttgtc ggccgagcgg gcggatgaac gggacttatg
     4021 attgttaggg aattggcgca ttctcttccg ccggcaaggg tatcctggaa agccttaata
     4081 ccagacgcaa ggcatcgttc acagcctggt cggtcgggaa tgccgcggcc acgtccggtt
     4141 ccagcaatac gacgttcgtc cctgcctcat agcgggcagc atacttgccg cgagttgcat
     4201 ctttcagtaa cag
//
LOCUS       DAOMQG010000308         4208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8759_length_4208_cov_8.362389, whole genome shotgun sequence.
ACCESSION   DAOMQG010000308 DAOMQG010000000
VERSION     DAOMQG010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8759_length_4208_cov_8.362389"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLC06_13545"
     CDS             complement(<1..209)
                     /locus_tag="PLC06_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRO91517.1"
                     /translation="MFNITYSITTANINWEQLTADLQADDFDNGRTPDELRRSFENSA
                     VVAFARDENRVIGKARALSDGVCNA"
     gene            complement(273..>1503)
                     /locus_tag="PLC06_13550"
     CDS             complement(273..>1503)
                     /locus_tag="PLC06_13550"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRO91518.1"
                     /translation="PTMAQLTLTPTELRRLSIAAQCLEAPRPNPTKADVLDTIRRITC
                     LQVDPINVVARTQLLVLFSRLGAYDPADLEALLWDDKALFEYWAHAASIIPADDFALF
                     RPQMRRNLTVGSERLHDWLKTNEPFRRNLLDELTARGPLYVEEIESRAVESWPHKGGW
                     GGGSDVGIMLEVLWYRGQVSVTRRLGNGFGLKKQWGLMAHQVGERAAESPLTQPEAER
                     RAVERALPALGAARLRDVNNYFIRKEYKGLKKTLAALEAGGVIQQVAVRDGDGVDSNT
                     QWPGPWYLHNDMLPELERIRRGDWRPQTVLLSPFDNLIADRDRTELLFGMRYRVEIYT
                     PAARRQYGYYNMPILHGDRIIGRVDPKMDRKAGRLVVNAVHLEPGVALDGETRAAVDG
                     AIASLAGFLGAEGIDGP"
     assembly_gap    1504..1603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1610..1981
                     /locus_tag="PLC06_13555"
     CDS             1610..1981
                     /locus_tag="PLC06_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HRO91519.1"
                     /translation="MSEKPAEYYLDLPYTIELVNDTSNPNNPVWFAAVRELRGCMTEA
                     DSFDEAVRQIRDAMHVWITDAIESGDEVPEPRGEEAFSGKFSVRLPKSLHRDLVARAE
                     QEGVSLNQYITAALGRAVGLG"
     gene            complement(2133..3071)
                     /locus_tag="PLC06_13560"
     CDS             complement(2133..3071)
                     /locus_tag="PLC06_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRO91520.1"
                     /translation="MDRTFIADLHNHTTASDGEYTPTELVSKAAELGLTALGVTDHDT
                     INGLDEALAAGAAAGVRVVPGVEVSLRFKRPYFTGTLHYLLYIPYDLLGDAEFRAAAE
                     AIFSQGRGGGLVRARVAAINAEFGPAGTTPLLERELTAEEIEALADNVTRRHFALALS
                     NNHGLSKEQVNLLIGNDSRAYIPSGIDPATLTPLLHAYPQLVRVFAHPAAGSYPGESL
                     YNEVLPPIEVVDTLMPEFLDNELLGLDGLEVQYPGHVAEHRALMAEWAERYGLIQTGG
                     SDCHDRVNRPLGVAGVDAAGLDALLGLLGEKQSAAA"
     gene            complement(3351..>4208)
                     /locus_tag="PLC06_13565"
     CDS             complement(3351..>4208)
                     /locus_tag="PLC06_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRO91521.1"
                     /translation="WTVTPISRGAGVITSLVRADGIVRVPRFSEGHDAGAAVTVHLYR
                     EPHEIERTIVSIGSHDLILDVAAQFLAARHDGLRLSSANVGSLGGLVALRRGEAHLAG
                     SHLLDTATGDYNTSYIHRYLPGQDVVVVTLAGREQGWIVPPGNPKELSGWADAARADL
                     GIVNRQRGAGTRVLLDYELGKLGIDPAAVRGYEREEYTHLAVAAAVASGAADAGLGIR
                     AAARALHLDFVPLAHEAYELVIPREHYESDLLRPLLDLLHDAEFRAAVAAMPGYDLTH
                     MGATREIAS"
BASE COUNT          673 a         1332 c         1383 g          720 t
ORIGIN      
        1 taggcattgc acacgccgtc ggacagggcg cgggccttgc cgatgaccct gttttcatca
       61 cgggcaaagg ctaccacggc gctgttctcg aacgagcgac gcagttcgtc gggcgtgcgg
      121 ccgttatcga agtcgtcggc ctgcaagtcg gccgtcagtt gttcccagtt gatgttcgcg
      181 gtagtaatgg agtaggtgat gttgaacatg gctgcattgt agcgcagaca ttcctgtctg
      241 cgaaagggcg ggcgcggaca ggaatgtctg cgctacgggc cgtcgatccc ttccgccccc
      301 aaaaaccccg ccagcgacgc aatggccccg tcgacggccg ctcgcgtctc gccgtcgagc
      361 gccacccccg gctccagatg cacggcgttg acgaccagcc gccccgcctt gcgatccatc
      421 ttcgggtcga cgcggccgat gatgcggtcg ccgtggagga tgggcatgtt gtagtagccg
      481 tattgccgcc tggccgccgg ggtatagatc tccacgcggt agcgcatgcc gaacagcagt
      541 tccgtccggt cgcggtcggc gatgagattg tcgaacggcg agagcagcac ggtttgcggc
      601 cgccagtcgc cgcggcggat gcgctccagt tcgggcagca tgtcgttgtg gagataccac
      661 gggccgggcc attgggtgtt gctatcgacc ccgtcgccgt cacgcacagc gacctgttgg
      721 atgacgccgc cggcttccag cgcagccagc gtcttcttca gccccttgta ttccttgcga
      781 atgaaataat tattcacgtc gcgcaggcgg gccgcgccca gcgccggcag cgcccgctcc
      841 accgcccggc gctccgcttc cggctgcgtc aggggcgatt cggccgcgcg ctcccctacc
      901 tgatgggcca tcagccccca ctgcttcttc aggccgaagc cgttgcccag ccgccgtgtc
      961 acgctgacct ggccgcgata ccacagcacc tcaagcatga tgcccacatc gctgccgccg
     1021 ccccagccgc ccttgtgggg ccagctctcg acggcgcggc tctcgatctc ctccacgtag
     1081 agcggccccc gcgccgtcaa ttcgtcgagc aggttccggc ggaacggctc gttggtcttc
     1141 agccaatcgt gcaatcgttc gctgccgacc gtcaggtttc gtcgcatctg cggccgaaag
     1201 agggcgaagt cgtcggccgg gataatcgag gcggcgtggg cccaatactc aaacagggcc
     1261 ttgtcgtccc acagcagggc ctccaggtcg gccgggtcat acgcgcccaa ccggctgaac
     1321 agcaccaata gttgcgtgcg ggccacgacg ttgatcggat cgacttgcag gcaggtgatg
     1381 cggcggatgg tgtcgagcac gtcggctttg gtcgggttcg gccgcggggc ctcaaggcat
     1441 tgggcggcga tggacaggcg gcggagttcg gtgggggtga gggtgagttg ggccatggtg
     1501 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgagaa tgagtgaaaa
     1621 accggcagaa tactatctgg acttgcctta caccatcgaa ctggtgaacg acacatccaa
     1681 cccgaataat cccgtctggt ttgccgccgt gcgcgagttg cggggctgca tgacggaagc
     1741 cgacagcttt gatgaggcgg tgcggcagat tcgcgacgcc atgcacgtct ggattaccga
     1801 cgccatcgag tcgggcgacg aggtgcccga acccagaggc gaggaagcgt tcagcggcaa
     1861 gttttcggtg cgtctgccca aatcgctgca ccgcgacctc gtggcgcgcg ccgaacagga
     1921 aggcgtgagc ctgaatcagt acatcacggc cgcgcttggt cgggcggttg ggttgggtta
     1981 gcaggggcga cggccgcggc gaaattatct acgtcatagg gacgcctgga tcgaggcagc
     2041 cagggccgaa ggaaggccgg taccgtcgcc tcggtaccgg ccggtaatct atcaggctgt
     2101 tttagcggag tagttaaagg caaattatcc gatcaggccg cggccgattg cttctccccc
     2161 agcagcccca gcagcgcatc cagccccgcc gcgtctaccc ccgccacccc cagcggccga
     2221 ttcacccggt cgtggcaatc ggaaccgccg gtctggatga ggccgtagcg ctcggcccac
     2281 tcagccatca gcgcccgatg ctcggccacg tggccggggt attgcacctc cagcccgtcc
     2341 agccccagca gttcgttgtc gaggaactcc ggcatgagtg tgtccaccac ctcgatgggc
     2401 ggcagcacct cattgtagag gctctcgccg gggtaggagc cggccgccgg gtgggcgaag
     2461 acgcgcacca gttgcggata agcgtgcagc aggggcgtca gcgtggccgg gtcgatgccc
     2521 gacgggatat aggcccggct gtcgttgccg atgagcaggt tcacctgctc cttgctcagc
     2581 ccgtggttgt tcgacagggc cagggcgaaa tggcggcggg tcacattgtc ggccagcgcc
     2641 tcgatctcct cggccgtcag ctcgcgctcc agcagcggcg tggttccggc cgggccgaac
     2701 tcggcgttga tggcggccac ccgcgcccgc accagcccgc cgccgcgccc ctggctgaag
     2761 atcgcctcgg ccgcggcgcg gaattcggcg tcgcccagca ggtcataagg gatgtagagc
     2821 agatagtgga gcgtgccggt gaagtagggg cgcttgaagc gcagcgagac ctcgacgccg
     2881 ggcacgacgc ggacgcccgc cgccgcgccc gcggccagcg cctcatcgag gccgttgatc
     2941 gtgtcgtggt cggtgactcc cagcgccgtc aggccaagtt cggcggcctt gctgaccagt
     3001 tcggtggggg tatattcacc gtcggaagcg gtggtatggt tgtgcaaatc ggcaataaac
     3061 gtgcgatcca tcgcagtgtt gtctcctcgt cgtttctttt ggaaaaatct ggcggattcc
     3121 tgctcgtagt gcgcaacttt tggatattta tcaattaatg cggtgtagcc cggttgcctg
     3181 atcctccgga tgtggcgcaa tctgacagat tgcgtcttcg ccggggatgc aaggcgcaat
     3241 ctaacagatt gcgctacaac gctatatcgc attaatttgt taacatgcaa aagcgcctga
     3301 ctacaagcaa gctatagcgt agacgctttg gatgacaaac caccttacgg tcatgatgcg
     3361 atctcgcgcg tcgcgcccat gtgggtcagg tcgtagccgg gcatggccgc cacggccgcg
     3421 cggaactccg cgtcgtggag caggtcgagc agcggccgca gcaggtcgct ctcgtagtgt
     3481 tcgcggggga tgaccagttc gtaggcctca tgggccagcg gcacgaaatc gagatggagc
     3541 gcccgcgcgg cggcgcgaat gcccagcccg gcgtcggccg cgcccgaagc caccgccgcc
     3601 gccaccgcca gatgggtgta ctcctcgcgc tcatagccgc gcacggccgc cgggtcgatg
     3661 cccagcttcc ccagttcgta atcgagcagc acgcgcgttc ccgcgccgcg ctgccggttg
     3721 acgatgccca gatcggcgcg ggcagcgtcg gcccagccgc tcagctcctt cgggttgccc
     3781 ggcgggacga tccagccctg ctcgcgcccg gccagcgtca ccacgacgac atcctgcccc
     3841 ggcaggtagc gatggatgta gctggtgttg tagtcgcccg tggccgtgtc cagcaggtgg
     3901 ctgcccgcca gatgggcctc gccgcgccgc agcgccacca gcccgccgag gctgccgaca
     3961 ttggccgagc tgaggcgcag gccgtcgtgg cgcgccgcca ggaactgggc cgccacgtcg
     4021 agaatcaggt cgtggctgcc gatggagacg atggtgcgct cgatctcgtg cggctcgcgg
     4081 tagaggtgga cggtgacggc cgcgcccgcg tcgtggccct cgctgaagcg gggcacgcgc
     4141 acgatgccgt cggcgcgcac gagcgaggtg atgacgcccg cgccgcggct gatgggcgtg
     4201 acggtcca
//
LOCUS       DAOMQG010000309         4204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8770_length_4204_cov_8.756327, whole genome shotgun sequence.
ACCESSION   DAOMQG010000309 DAOMQG010000000
VERSION     DAOMQG010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8770_length_4204_cov_8.756327"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..436
                     /locus_tag="PLC06_13570"
     CDS             11..436
                     /locus_tag="PLC06_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRO91522.1"
                     /translation="MSANPPQFVDTNILIYAHDCSAGPKHDRARELLQTLWRERSGCL
                     SIQVLQEFYVNVTRKVAHPLSSESAAQIIADLAVWQIHRPDTNDVLGAIRLQGRLQLS
                     FWDAMIMTSARRLGCEIVWSEDLSHGQVYEGVEVRNPFI"
     gene            complement(503..>4204)
                     /locus_tag="PLC06_13575"
     CDS             complement(503..>4204)
                     /locus_tag="PLC06_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91523.1"
                     /translation="CSSVLNIQLRDGKGWSGIAYWGSPGNCNIGIDAPMIRDGVGTGD
                     DGVVFHEVQHYTQYSYNNGCYADFQPLYSSGNAWIEGWANLAGKNATNAANDADYRVS
                     SYDSENSYYDLDYTDLHAQYLIEQFGGNGAPGDSAFGVKAVYEHYRQCDQYDDIFVMD
                     ETIQSLTGGAKTEKWAFVDFEAAYYAYPWADPVAQPELVFPDADDKPTGTPAFAQNVN
                     MAAGSQSWVQSSPEDWTGRYYRINPQSGCAFVELHAETQPPGGEIGINFMAVNTGAPK
                     VQRSAHIGDNATRLFAGFPANNQLVVNVTSFDSVMTYQVTATCVTPTLNILQPIHNPG
                     HAMVGAPNNPVATLTRFEVTSGGLPVSGLAASIVSFDAEGSAATLVPGSFQEVGPGQY
                     WAAIVPPTKPLGTTFVDYKVCLNTSICDSENDALLYVDPGNVDTVYLFDESNSMNTED
                     TPGEGTRITNAKRAGRVLPDLLRDGDRVGIIGHGALDVPPGCGLPGGSGNCPADNITR
                     LARIDNVNVPGDIAVAKAGVGAVTTRLVWTNTGQGLLSAKDMLLANPGNTNPDHIILL
                     SDGQENVHPLYDTPAVRGVLHAAGVCVDTIGLGPEAPGALLAQIAAQNCGTYTPVPTS
                     GLGTSLRAVDAVAVAHAQLIAMGAPDELADSIAAVAAADFYPAQLGIANANEYLDTAA
                     QDASRLFHFLHQAVPPQDWSTHFAVIDESVSSFQFTTVGKLEDDKGIRWVELLMPGMD
                     PAQDRWIPISPPDATTPTDWDIRNDPFEDVVIVNKPQPGLWGFRVRYAEVPGDYIMNL
                     AVQTPIRLEGNLLGLTNGQATAGDTVPIVATLMDHKGLLPGALVAAAVANEGGTDVML
                     LFDDGNHNDGGAADGIYGFPYSLTDHGGSYAVRIVALFPNPTNAGENLFREWNGGFWI
                     DGPQDDGKWDGDSDGDGMPDDWEKRCKLIVGQNDARADPDRDGLLNFEELRLGTSPCI
                     ADTDRGGERDGSEVQGGRNPLWPDDDKAFKLTGIVILPFNEAIVVNWSQRPDTNVNVR
                     LCISMTAGVLGNCQDMGATGEFKLDGLKNGQTYYVTLYGEGPDGAQGAYSDQIPVTPA
                     EDPIPPQGAFYIGGSNVSEGGDVAMSRSVTLFVDAVDTDSEYDGPAGLGSHTVPHNLV
                     GPETAGMFTVSGNVEMRFANTLAGIETAPWEPLAPTKAWTLLCAPGDMCTVYGQFRDG
                     AGNESLIIDQKIMLSAEPPKTYLPAIFKP"
BASE COUNT          710 a         1324 c         1396 g          774 t
ORIGIN      
        1 gacgaactcc atgagcgcta atcccccaca gtttgtcgat accaacatcc tcatttatgc
       61 ccatgactgc tcggccgggc caaagcacga ccgggcacga gaattgttgc aaacgctatg
      121 gcgggagcgc tccggctgcc tcagcattca ggtcttgcag gagttctacg ttaacgtcac
      181 tcgcaaggtc gcccatccat tatcatcgga atcggcggcg cagatcatcg ctgacctggc
      241 cgtctggcag atccaccggc cggacacaaa cgacgtgctg ggggcaatcc ggcttcaagg
      301 ccgtctgcaa ctgtcgtttt gggacgccat gatcatgacc agcgctcgcc ggctgggctg
      361 tgaaatagta tggtcggagg atttgagcca cggacaggtc tacgaaggag tagaggtccg
      421 caatccgttt atctaaaaaa ggccctccga gacgcatccc ggagggcctt tccatataga
      481 aggggagaag cgaaaccaac ccctacggct tgaaaatcgc cggcagatac gtcttcggcg
      541 gctcggccga cagcatgatc ttctgatcga tgatcagcga ctcgttgccc gcgccgtcgc
      601 ggaactgacc gtagacagta cacatgtcac cgggggcgca cagcagcgtc cacgccttcg
      661 tgggcgccag cggttcccac ggcgcggttt cgatacccgc caacgtgttg gcgaagcgca
      721 tctccacgtt gccgctgacg gtgaacatgc ccgcagtctc cgggccgacc agattatgcg
      781 ggacggtatg ggaaccaagc ccggccgggc cgtcatactc gctgtcggta tccaccgcgt
      841 cgacgaacag agtgacgctg cgcgacatcg ccacgtcgcc cccttcacta acgttcgagc
      901 cgccgatgta gaacgcacct tgcggcggga tcgggtcttc cgccggcgtc accgggattt
      961 ggtcggagta agccccttgc gcgccgtccg gcccctcgcc atagagcgtg acgtagtagg
     1021 tctggccgtt cttcagccca tcgagcttga actcgccggt cgcgcccatg tcctgacaat
     1081 tgcccagcac ccctgccgtc atactgatgc acaggcgcac gttgacgttg gtgtccggtc
     1141 gttgcgacca gttaacgacg atcgcttcgt tgaagggcag gatcacgatg ccggtgagct
     1201 tgaacgcctt gtcatcgtcg ggccaaagcg ggttgcggcc gccctgcacc tcgctgccgt
     1261 cccgctcacc gccgcggtca gtgtcggcga tgcacggcga cgtgcccaat ctcagctctt
     1321 cgaagttcag caggccgtcg cggtcaggat cggcgcgagc gtcgttctga cccacgatca
     1381 acttgcagcg cttctcccag tcatcgggca tgccgtcgcc gtcggagtcg ccatcccact
     1441 tgccgtcatc ctgcgggccg tcgatccaga acccgccgtt ccattcgcgg aacaggttct
     1501 cgccggcatt ggtcgggttg gggaacaagg ccacgatgcg aacggcatag gagccgccgt
     1561 ggtcggtcag gctatagggg aagccgtaga tgccgtcggc cgcgccgccg tcgttgtggt
     1621 tgccgtcgtc gaacagcagc atcacgtcgg tgccgccctc gttggccacg gccgccgcga
     1681 caagcgcccc cggcaacagc cccttgtgat ccatgagcgt ggccacgatc ggcaccgtgt
     1741 cgccggctgt cgcctgcccg ttcgtcaggc ccagcaggtt gccttccagc cggatcggcg
     1801 tctggacggc caggttcatg atgtagtcgc ccggaacctc ggcatagcgc acccggaacc
     1861 cccacaggcc cggttgcggc ttgttgacga tgaccacgtc ctcaaacggg tcgttgcgga
     1921 tgtcccagtc ggtcggggtg gtggcgtcgg gcgggctgat gggaatccag cggtcttgcg
     1981 ccgggtccat gccgggcatg agcaactcca cccagcgaat gcccttgtcg tcctccagct
     2041 tgccgacggt cgtaaactgg aagctgctga ccgattcatc aatcacggcg aagtgggtgc
     2101 tccagtcctg gggcggcacg gcctgatgga gaaagtggaa cagtcgcgat gcatcctgag
     2161 cggccgtgtc aaggtactca ttggcgttgg cgatgcccag ttgggcgggg tagaagtcgg
     2221 ccgccgccac cgcggcgatg gagtcggcca gttcgtccgg tgcgcccata gcgatcagct
     2281 gcgcgtgggc gacggccacg gcgtctactg cacgaaggct ggtgcccagc ccggaggtcg
     2341 gcacgggcgt ataggtgccg cagttctggg cggcgatctg ggccagcagc gcgcccggcg
     2401 cttccggccc cagcccaatc gtgtcgacgc agacgccggc ggcgtgcagc acgccgcgaa
     2461 cggccggcgt gtcgtagagc ggatgcacgt tctcctgccc gtcactgagc agaatgatgt
     2521 ggtcgggatt ggtgttgccg gggttggcca gcagcatatc cttggcgctg agcagtccct
     2581 gaccggtgtt ggtccagacg agccgcgtgg tgaccgcacc gacgccggcc ttggccacgg
     2641 cgatgtcgcc ggggacgttg acattgtcga tgcgggccag ccgggtgatg ttgtcggccg
     2701 ggcagttgcc cgaaccgccc ggcaagccgc agcccggcgg aacatccagc gcgccgtggc
     2761 cgatgatgcc cacccgatcg ccgtcgcgca gcagatcggg cagaacccgc ccggcgcgct
     2821 tggcgttggt gatgcgcgtg ccctcgcccg gcgtgtcttc ggtgttcatc gagttcgact
     2881 cgtcgaagag atagacggta tcgacattgc ccgggtccac atagagcagg gcgtcgttct
     2941 cggaatcgca gatcgaagta ttaaggcaga ccttgtaatc gacgaaggtc gtccccagag
     3001 gcttggtggg cgggacgatg gccgcccaat actgcccggg gccgacttcc tggaatgagc
     3061 cgggcaccag cgtggcggcg ctgccctcgg cgtcgaatga gacgatcgac gcggcgaggc
     3121 cgctgaccgg caggccgccg ctggttactt cgaagcgggt cagggtcgcc accgggttat
     3181 tgggcgcgcc gaccatcgcg tggccggggt tgtggatcgg ctgcaggatg ttgagcgtcg
     3241 gcgtgacaca ggtcgccgtg acctgatacg tcatcacgct gtcgaagctg gtgacgttga
     3301 ccaccagctg gttgttggcc gggaacccgg cgaagagccg cgtggcgttg tcgccgatgt
     3361 gggccgagcg ctgcaccttc ggcgcgccgg tgttgacggc catgaaattg atgccgatct
     3421 ccccgccggg cggctgcgtc tcggcgtgaa gctccacgaa ggcgcagccc gactggggat
     3481 tgatccggta gtagcggccg gtccagtcct cgggcgagga ctgcacccag ctctggctgc
     3541 ccgcggccat gttgacgttt tgggcgaagg ccggcgtgcc ggtgggcttg tcgtcggcgt
     3601 cggggaagac cagctccggc tgcgcaaccg gatcggccca ggggtaggcg tagtaggcag
     3661 cctcgaaatc gacaaacgcc cacttttccg tcttggcccc accggtcagc gactgaatgg
     3721 tctcatccat gacgaagatg tcgtcgtact ggtcgcactg ccggtaatgc tcatagaccg
     3781 ccttcacgcc gaaggccgaa tcgcccggcg cgccgttgcc gccgaactgc tcgatgagat
     3841 attgcgcgtg gaggtcggtg taatcgaggt cgtaatagga gttctcgctg tcgtaggacg
     3901 acacgcggta gtcggcgtcg ttggcggcat tggtggcgtt cttcccggcc agattggccc
     3961 aaccctcgat ccaggcattc ccggatgagt agagcggttg aaagtcggca tagcagccgt
     4021 tattgtagga gtactgggtg tagtgctgca cctcgtggaa gacgacgccg tcgtcgccgg
     4081 tgcccacgcc gtcgcggatc atcggcgcgt cgatgccgat gttgcagttg ccgggcgaac
     4141 cccaataggc gatgcccgac cagcccttgc cgtcgcgcaa ctggatgttg aggacggagc
     4201 tgca
//
LOCUS       DAOMQG010000310         4192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8802_length_4192_cov_10.085328, whole genome shotgun sequence.
ACCESSION   DAOMQG010000310 DAOMQG010000000
VERSION     DAOMQG010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8802_length_4192_cov_10.085328"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PLC06_13580"
     CDS             <1..1883
                     /locus_tag="PLC06_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91524.1"
                     /translation="GLMVEPNPAQARIVLPPMEQAIDMATVILMVWGLAPFAMSRPRL
                     ADGFLLISLVVVAVMFLFFFQDWQNRVTATGVATAYVGTIQAYVWGVLQMAILAMGLI
                     WSLMERSSRLTLRPIIIAVVLLAHVANFFNYPEIIPSGTEIVYWLRLGYLVAFPLWAV
                     QAYRDNIRGLLTPVEAERLQAAQLAHNLQLSTRVIGARLPEMRLEQALDMVTGLVPAR
                     LTAIGLIDEKNPQRVVFGHLRPTPARDTGYGRQINLSDHAAFRLAYEQQRGIELLPRG
                     VGARQAHELSQQFNAGPFGPVFVEPLVVANHCLGFLMLAAPPTAPSWSERDRAIIPGM
                     AAFIAQAIANSRHGDFEAAGVSSQAVSPVLPAAITLARAADRDTEKERLMLELAETRR
                     MLSAAEERTRQAETMAVFLQQRDPGQPTAARPQVTQSALGPAAEYAAGAILPMLRQKN
                     LKLDMAVDQDLPLAAVKETVLRQLVLSLLENSCRASRENGTIFVRADLISTNGGGPAS
                     GRAVALSVSDSGSGIRAEDRDRVFDSQYYLSGGRPIVGLGDNSANLAVVQKLAQASGG
                     DLQFESKLGTGTTFTLRLPAAEVRPWTLMKVKQQMEASAPKRPAAEIEAAAGSTTVGE
                     AG"
     gene            1888..2928
                     /locus_tag="PLC06_13585"
     CDS             1888..2928
                     /locus_tag="PLC06_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRO91525.1"
                     /translation="MDTDRSAQRRSWQVNLLFTSGVMITVLLALILAQLDALQARAAL
                     DRPSLSIVNIQATAIAVGNIIPLEFEAKLPDAAAELVAPVPDDLNPRPGFITGVSDEG
                     VIYTVCGAVPEGWLLYTVQPGETLASLAAGTETTAAELVAANCLAGELSVGMQLLVPR
                     QPAVSLCGPPQWWVRYQVQRGDTIGALAVLRGTTVDEVLRANCRDSSDLQAGQSIFLP
                     PGAQPGSIAPIPQPSLTPLPLPTVVPTLPPPTATTPPDQPPPPSPTAPIVASPVPPTA
                     TPPPLPTIGPPTVPPPAPTEPPPTQPPPTEPPPTQPPPTEPPPTEPPPTAPPPTEPPP
                     PTEQPAPVGEGG"
     gene            3117..>4192
                     /gene="rfbH"
                     /locus_tag="PLC06_13590"
     CDS             3117..>4192
                     /gene="rfbH"
                     /locus_tag="PLC06_13590"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HRO91526.1"
                     /translation="MGDIEELRQSILDQVAEYYEKAHQNRNFEPGKTTVNYAGRVFDE
                     TEMQNMVSSVLDFWLTAGPYAEEFEKQLGAFIGVREVVPVNSGSSANLVAITTLCSRQ
                     LPDGLRPGDEVIVPAVSFPTTINPIIQNRLMPVFIDSCLGDLNLDVSQLEEAVSPRTR
                     ALVFAHTLGNPANMDEVMAFVKRHDLFLVEDTCDALGSKWDGRNVGTFGELATLSFYP
                     AHHITMGEGGAVYTDRPKLAKIARAVRDWGRDCWCGYDNPVNGKCGIRFERVIPGIPG
                     FYDHRYFYSEIGYNLKLTDPQAAMGLAQLVKLPDFIRRRKENFAYLYEELKQFSEFLM
                     LPTWHPKADPSWFSMPLVVRDEAP"
BASE COUNT          774 a         1335 c         1293 g          790 t
ORIGIN      
        1 tcggcctgat ggtggagccg aatccggctc aggcgcgtat cgtgttaccg ccaatggaac
       61 aggcgatcga catggcgacc gtgatcctga tggtgtgggg actggcgccg ttcgccatga
      121 gtcggcctcg tctggccgac gggtttctcc taatcagtct cgtcgtcgtg gccgtcatgt
      181 tcctgttttt ctttcaggat tggcaaaacc gtgtgacggc gaccggcgtg gccacagcct
      241 atgtcggcac gatccaggcc tacgtctggg gcgtgctgca gatggcgatc ctggccatgg
      301 ggctgatctg gtcgcttatg gaacgcagca gccggttgac attgcggccg atcattattg
      361 ccgtcgtgtt gctggcccat gtggccaatt tcttcaacta cccggaaatc atcccttccg
      421 gcacggaaat agtttactgg ctgcgattgg gttacctggt tgctttcccc ttgtgggccg
      481 tgcaggccta tcgcgacaac attcgcggct tgctgactcc agtcgaagcc gagcgactgc
      541 aggccgcgca actggcgcat aacctgcaat tgtcaactcg cgtcatcggc gcgcgccttc
      601 ctgagatgcg cctggagcag gctctcgata tggtgaccgg cctggtaccc gcgcggctga
      661 cggctatcgg cctcatcgat gaaaagaatc cgcagcgggt tgtcttcggg cacttgcgcc
      721 cgactccggc tcgcgatacc ggctacggcc gccagatcaa tctttccgat cacgccgcct
      781 ttcgcctggc atatgaacag cagcgcggca tcgaattgct gccgcgtggt gtcggcgcgc
      841 ggcaggccca cgaattatca cagcagttca acgccgggcc gttcggccct gtgttcgttg
      901 agccactggt tgtggcaaac cattgtctgg ggttcctgat gctggctgcg ccaccaaccg
      961 cgcccagttg gtcggagcgc gaccgggcca tcatccccgg catggctgcg ttcatcgccc
     1021 aggccatcgc caatagccgt catggagatt tcgaggcggc cggcgtctct tcccaggccg
     1081 tctcacctgt gttgccggcg gcgatcaccc tggcgcgcgc ggctgacagg gataccgaga
     1141 aggaacgcct catgctcgaa ttggccgaga cgcggcgaat gctctcggcg gccgaagaac
     1201 gcacgcgcca ggccgagacg atggccgtct ttttgcaaca acgggacccg ggccaaccca
     1261 cggccgcccg accgcaggta acgcagtcgg cgcttggccc ggcggcagag tatgcagccg
     1321 gggcgatcct gcctatgttg cggcagaaga atctgaagct ggatatggct gtcgatcagg
     1381 atctgccgtt ggcggccgtc aaggagacgg tgttgcgcca gctggttctc agcttgttgg
     1441 agaactcctg ccgggcatcc agggaaaacg ggactatctt tgtgcgggcc gatctcattt
     1501 cgaccaacgg cggcggcccg gcatccggcc gcgcggtggc cttgtcggtg tccgatagcg
     1561 gttccgggat tcgcgccgag gaccgcgatc gcgtattcga ttcgcaatac tatctctccg
     1621 gcggccggcc catcgtcggg ttgggcgaca acagcgccaa tttggccgtc gtacaaaagc
     1681 tggcccaggc cagcggcggc gatctgcaat ttgagagcaa gctgggcaca gggacgacct
     1741 ttacgttacg tctgcccgcg gccgaggtgc ggccgtggac cttgatgaaa gtcaagcagc
     1801 aaatggaagc gagcgccccc aaacgtcctg cggcagagat cgaggccgcg gctggaagca
     1861 ccacggtcgg cgaggcgggc tgaagttatg gataccgatc ggagcgcgca gcgccgcagt
     1921 tggcaggtca acctgctctt caccagcggg gtgatgatca ccgtgctgtt ggccctgatt
     1981 ctggctcaac tggacgcgct ccaggctcgc gcggcgctcg atcggccgag tctgtcgatc
     2041 gtcaacattc aggcgacggc catcgccgtc ggcaacatca tccccttgga gttcgaggcg
     2101 aaattacccg atgctgccgc ggaactggtt gcgcccgtgc ccgatgatct gaacccacgt
     2161 ccggggttta tcaccggcgt cagtgatgaa ggagtcatct ataccgtgtg cggggcggtg
     2221 ccggaaggct ggctgctcta cacagtccag ccgggcgaga cgctggcctc gctggccgcg
     2281 gggacggaga caaccgcggc cgaactggtt gccgccaact gtttggccgg ggagttgtcg
     2341 gtcgggatgc aactgcttgt gccacggcaa ccggccgttt ctctctgcgg gccgcctcaa
     2401 tggtgggtgc gctatcaggt gcagcgaggc gacacgatcg gcgctctggc cgtgctgcgc
     2461 ggcacgactg tggatgaggt gttgcgcgcc aattgccgtg attcgtccga tttgcaggct
     2521 gggcaatcca tttttctgcc accgggggcg caaccgggat caatcgcgcc aattccccag
     2581 ccgtccttga cgccgctgcc gctgccgaca gtggtcccga ccttgccgcc acccacggcg
     2641 acaacgccgc ccgaccagcc gcccccaccc tcaccaaccg cgccaattgt tgccagcccg
     2701 gttccgccaa ctgccacacc accgccgctg ccgacgatcg ggccgccaac agtgcccccg
     2761 ccggcaccca cagagccgcc accgacacaa ccaccgccga cggaaccgcc accaacacag
     2821 ccaccgccga cggaaccacc accgacggag ccgccaccga cggctccccc gccaacagag
     2881 ccgccaccac cgacggagca gcccgcgcct gtcggagaag gtggataggg tggaagccac
     2941 cggggcgact tggagatggc tttcgctgtg atataatcca ccctcggcct cggatagtct
     3001 gggccgcccg ggcttataca acgagtagag acggccaatc cgacagctac tacagccgac
     3061 tgcgttccac aacgctaccg gcgtcgacac catcagtgtc aaagagaaaa cgtagaatgg
     3121 gtgatatcga agagctgcgt caatcgatac tggaccaagt cgccgagtat tacgaaaaag
     3181 ctcaccaaaa ccggaacttt gaacccggaa aaacaactgt aaactacgcc gggcgcgtct
     3241 ttgacgagac ggagatgcag aacatggtca gctccgtgct cgatttctgg ctcacggcgg
     3301 gaccatacgc cgaagagttc gagaagcaat tgggcgcttt catcggcgta cgcgaggtgg
     3361 tgccggttaa ttccggctca tcggccaacc tggtcgccat cacaacgtta tgctcgcgcc
     3421 aattgccgga tgggctacgc ccgggcgacg aggtcattgt cccggccgtc agtttcccga
     3481 ccaccatcaa tccgatcatc cagaaccggc tcatgccggt tttcatcgac agttgcctgg
     3541 gtgatctgaa tctcgacgtc tcgcaactgg aagaggcggt gtcgccgcgc acccgagcgc
     3601 ttgtctttgc ccacacgctg ggtaatccgg ccaacatgga cgaggtgatg gcctttgtca
     3661 agcggcacga tctgttcctg gtggaagata catgcgacgc gctggggtcg aaatgggacg
     3721 gccgcaatgt cggcactttc ggcgagttgg cgacgctcag cttctacccg gcccatcaca
     3781 tcaccatggg cgaaggcggc gcggtctata ccgaccggcc aaagctggcc aagatcgctc
     3841 gcgctgtccg cgactggggg cgggattgct ggtgcggcta cgacaacccc gtcaacggca
     3901 agtgcggcat ccgctttgag cgggtgatcc ccggcatccc cggcttctac gaccaccgct
     3961 atttctattc cgagatcggc tataacctga agttgaccga tccccaggca gccatggggc
     4021 tggcccagtt agtgaagctg cccgatttta ttcgccgtcg caaggaaaac ttcgcttatc
     4081 tctatgagga actgaagcaa ttcagcgaat tcctgatgtt gccgacatgg cacccgaagg
     4141 ccgacccgtc ctggttctcc atgccgctgg ttgtgcgcga cgaagcgccc tt
//
LOCUS       DAOMQG010000311         4182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8837_length_4182_cov_10.050157, whole genome shotgun sequence.
ACCESSION   DAOMQG010000311 DAOMQG010000000
VERSION     DAOMQG010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8837_length_4182_cov_10.050157"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4182)
                     /locus_tag="PLC06_13595"
     CDS             complement(<1..>4182)
                     /locus_tag="PLC06_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRO91527.1"
                     /translation="ANGEVIWQFASQENSTAIVNPQMVVDDFDGDGANEIVMGVFTAR
                     RFSELYYLDDGIVKWQQAISRRITHIAKAPFGPSGNAIAVGTNFGQLELYSSTGDPLW
                     YRTVNKPITSLVTIQLPEGPALALGTASGSVIAYSAEGRRLWTNHLVKDADRRVLTLL
                     PAGGRTATGQTPLAAILEPATTGSELADLLLLGNNGQTLAKLNDTDLPDLTRLVDVNH
                     DGHYELLLARFATLQLIGLGMGDSEYIQEWDYVLDAEPTAMLIQDLDEDGEDEIVVGT
                     RDGRLHSLGMDRAIRWLNAPGGVISLLDRVRYASGDPPHIVVVRRQRPPADFVTDDES
                     VLSWLELREATGDRLWEITVPANITSLLIDDKLGAGESSIIIGTQSGDVSSFNLDGNQ
                     RWTFSISDSVGAVQQLIQFRPIPGEPQVILAVGQYSIVRLIEDGGSISVKHFATFDEP
                     VQAVYGMQQLDRGESTVKLIVFTRDGLVHGLNHEGVEMAHLGWPYKLPAPPRAIMPSD
                     QWGDEALQQGTAAFLLATSNGQLSHFVVDDNKPNISWSLNELGQIQSINWDDLDKDGQ
                     PDTGIFGSQDGHLWLYEQLQGRNPRLVLQQPLGSSTFALALLKRTSRQSPDLLAVTQN
                     GLIRLFSEEENRPPLLTQPVIESDQRQFTIGIQAADVENDFVTVQLELLDETINTWRP
                     VSEQQLPTGNGQLFWPGITAPEGSTKLNYRFRFSDGFYRGYVSPPAGPRIEPPEVSDS
                     TLPVLAGSIGLLIMVGFIAHVRQSQTPAAQADRFYDRLAQEPRSTLALVEQKYAMVNG
                     SPDFLLQLANRARHAEDTSLANLSDGLFLLANRPQAGLPIITRTLDDAADTDKKWDNL
                     AQRRMIYRTCQAMLEAPSITELGLVRPQFVHLLTELEEKKEWSPVLETLLPVLTNMRD
                     SDRVEAIDDRLVYLNQAAVRLRQVQDQINEFAPSVERTLVRSIARRWSGLLTAEIEEQ
                     RGRAELEVLLKTKRLAPNGQTHVAMEIRNTGRAPAENIVAILNENPAYSVRSEPQAIP
                     FLPAGHAQQVRFLIEPQAEERFRVGLSLIYDDRNQRRKTTAFGDMVHLLPPVRDFVPI
                     PNPYLPGTPLRKDSPLFFGREELFDFIAEHAGTQAQRNVFMLVGQRRTGKTSLLLRLG
                     EYLPSHLMPVYIDCQSLGVSPGMPALLQEFAWHIADVLSIHGLDLEVPEIDVWQADPT
                     RVFQRELLPAARRLLPANTTLLMIFDEFEAFESMVADGILPRTFFPYMRHLMQHSTGL
                     GFVFVGTRRLEEMSADYWSVLFNIALYRKIDFLSPQAAERLICEPVAPNLVYDDLALD
                     KILRVTAGHPYFLQLVCYTLVKQANERKTGYVTISDVNTALDDMLRLGEAHFAYLWQR
                     SNPVERAVLAAVAHL"
BASE COUNT          894 a         1147 c         1236 g          905 t
ORIGIN      
        1 tcaagtgggc gacggcagcc aatactgctc gttcaacagg gtttgatcgc tgccacaggt
       61 aggcaaagtg agcttcaccc agccgcagca tatcgtcaag agcggtgttg acatcagaga
      121 tcgtcacgta tccggttttt cgctcattgg cctgcttgac taacgtatag cagacgagtt
      181 gcaagaaata tgggtgcccg gcggtaacgc gtaatatttt atccagagcc aggtcgtcat
      241 agaccagatt gggagcaacc ggctcgcaga taagccgttc ggccgcctga ggactgagga
      301 agtcgatctt ccggtagagc gcgatgttga acaagaccga ccagtaatcg gcgctcatct
      361 cttccaaccg tcgcgtgccc acgaagacga accccagacc ggtgctgtgc tgcatcagat
      421 gccgcatgta agggaagaag gtgcgcggca gaatgccgtc ggccaccatc gactcgaagg
      481 cctcaaactc atcgaagatc atcaacagcg tcgtatttgc gggcaataaa cgccgcgccg
      541 ccggtaaaag ctcacgctgg aagacacggg ttggatccgc ctgccacacg tcaatctcag
      601 ggacctcaag atcgaggccg tggatgctca acacgtcagc gatatgccag gcgaattcct
      661 ggagcagcgc gggcatgccg ggcgagacgc cgagcgactg gcagtcgata tagaccggca
      721 tcagatggga tggcagatac tcacccagcc ggagcaagag cgatgtcttg cctgtgcggc
      781 gctggccgac cagcatgaag acattgcgct gagcttgtgt tccggcatgt tcggcgatga
      841 agtcgaataa ctcttcgcgg ccgaagaaca gagggctgtc cttgcgcagg ggtgttcccg
      901 gcagatatgg attaggaata ggcacgaaat cgcgcactgg cgggagcaga tggaccatat
      961 cgccaaaggc ggtggttttg cggcgctggt tccgatcgtc gtaaatgagc gaaagcccga
     1021 cccggaagcg ctcttccgct tgcggctcaa tcaggaaacg cacctgttgc gcatgacctg
     1081 ccggcagaaa ggggatcgcc tggggttcgc tgcgcacgga ataagccgga ttttcgttga
     1141 ggatggcgac gatgttctcg gccggggcgc gtcctgtgtt gcgaatctcc atggccacat
     1201 gagtttggcc gttgggggcc aatcgctttg tctttaacaa gacctccagt tcggcgcgcc
     1261 cgcgttgctc ctcgatttcg gcggtcagta ggcctgacca gcgccgggct atggatcgaa
     1321 ccagcgttcg ctctacgctg ggtgcaaatt cattgatctg gtcctgcacc tgccgcagcc
     1381 gaacggccgc ctgattgaga taaaccaacc ggtcatcgat ggcctcgact cgatcgctgt
     1441 cgcgcatatt agtgaggaca ggcaatagtg tttccaggac aggagaccat tccttctttt
     1501 cttccagttc ggtcaagaga tgcacaaact ggggccggac gagacccaat tcagtgatcg
     1561 aaggagcctc cagcatggcc tgacaggtac gataaatcat acgtcgttgg gccaggttgt
     1621 cccatttttt atccgtgtcg gcggcatcgt ccaacgtgcg cgtgataatc ggcaagcctg
     1681 cctggggccg atttgccagc aagaaaagcc cgtccgacag attagccagg ctggtatctt
     1741 cagcatgccg tgctcgattt gccaattgca acaggaagtc aggtgagccg ttcaccattg
     1801 catatttttg ttcgacaagt gcgagggtgc tgcgaggctc ctgagccagt cgatcgtaga
     1861 acctgtcagc ctgggccgcc ggggtctgtg actggcggac atgagcgata aacccaacca
     1921 ttatcaggag gccgatggat cccgccagta ccggcaatgt ggaatcgctg acctcaggcg
     1981 gctcaatcct ggggcctgcg ggtggactaa catagccacg atagaaacca tcgctaaacc
     2041 ggaatcggta gttgagcttc gtcgatcctt ccggggcagt gatgccgggc cagaataatt
     2101 ggccgtttcc ggttggcagt tgttgttccg agaccggacg ccaggtgtta atcgtctcgt
     2161 ccagcagttc cagctgaacg gtgacgaaat cattttctac gtcagcagcc tggatgccga
     2221 tcgtgaactg tcgttgatcg gattcaatga cgggttgggt gagaagcggg gggcgattct
     2281 cctcctcgct gaagaggcgg atcaagccgt tttgagtgac agccagcaag tctggggatt
     2341 gccgtgacgt gcgtttcagg agggcgagag cgaatgtgct gctgccaaga ggttgctgga
     2401 ggacgagccg agggttccgc ccctggagct gttcatataa ccacaaatga ccatcctgcg
     2461 atccaaagat gccggtatcc ggctgtccat ccttgtccag gtcatcccaa ttgatagatt
     2521 gaatttggcc taattcgttc agactccagg aaatattcgg cttattatcg tctacaacaa
     2581 aatgcgataa ttgaccattg ctggtagcga gcagaaaggc ggccgttccc tgttgaagcg
     2641 cctcatctcc ccattgatca gaaggcatga tcgcccgagg tggtgcgggc aacttgtagg
     2701 gccagcccaa gtgggccatc tctacccctt catgattcaa cccatgtacc agcccatccc
     2761 tggtgaacac gatgagtttg acggttgatt cccccctgtc gagttgttgc atgccataaa
     2821 ccgcctggac aggttcgtcg aaagtggcaa aatgctttac ggagatgctg cccccatcct
     2881 caatcaagcg cacgatgctg tattggccga ctgccaggat tacctggggc tctccgggta
     2941 tgggtctgaa ttgaatcagt tgctgaaccg caccaacaga atcgctgata gagaatgtcc
     3001 aacgctgatt cccatcgaga ttaaaggatg acacgtcgcc tgattgagtg ccgatgatga
     3061 tcgacgattc gccggcacct aatttgtcat cgatcagcag ggacgtaata ttggcgggga
     3121 ctgtaatttc ccatagacga tctcccgtcg cttcgcgcaa ttcaagccag cttaagactg
     3181 actcgtcatc cgtcacaaaa tcggccggcg gccgttgtcg tcgcacgacg acgatatgcg
     3241 gtggatcgcc cgaagcgtat cgcacgcggt cgagcaggct gatgactcct ccgggcgcgt
     3301 ttagccagcg aatagcgcga tccatcccca ggctgtggag ccggccatca cgcgtgccta
     3361 cgacgatctc gtcttcgccg tcctcgtcca ggtcctgtat cagcatggcc gttggctcgg
     3421 catcaagtac gtaatcccat tcctggatgt actcgctgtc acccatgccc aggccgatta
     3481 gctgtagtgt ggcgaaacga gccaatagca gttcgtaatg accgtcatga ttcacgtcga
     3541 caagccgggt cagatcgggc agatcggtgt cgttgagctt ggccagcgtt tggccgttat
     3601 tgcccagcaa caggaggtcg gccagttcgc tgccggtcgt cgccggttcc aggatagctg
     3661 ccagtggtgt ttggccggtt gccgtgcggc cgccggcggg cagcaatgtc agcacgcgcc
     3721 gatcggcatc cttgacgaga tggttagtcc ataagcgccg cccctcagcc gaataggcga
     3781 tcacgctgcc ggatgctgtg ccgagggcca gagccggccc ttcaggcagt tggatcgtta
     3841 cgaggctggt aatgggcttg ttgacggttc gataccatag cgggtcacct gtggaagagt
     3901 acagttccaa ttggccgaaa ttggtgccga cagctatcgc gttgccggac ggcccaaacg
     3961 gtgctttggc gatatgggtg atccggcgcg agatggcctg ctgccatttc acgatgccgt
     4021 catcgagata gtatagctcg ctgaagcggc gcgctgtgaa gacgcccatg acaatttcgt
     4081 ttgcgccgtc gccgtcgaaa tcgtctacca ccatctgtgg gttgacgatg gctgtggagt
     4141 tttcctgact cgcaaactgc caaataacct ccccattggc gt
//
LOCUS       DAOMQG010000312         4175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8855_length_4175_cov_8.276942, whole genome shotgun sequence.
ACCESSION   DAOMQG010000312 DAOMQG010000000
VERSION     DAOMQG010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8855_length_4175_cov_8.276942"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..721)
                     /locus_tag="PLC06_13600"
     CDS             complement(2..721)
                     /locus_tag="PLC06_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HRO91528.1"
                     /translation="MDVNKSIRYVFDDPQWISKVVIGALMSVLSFFILPAFILQGYVV
                     KVIRQVMNGDWDGLPEWDDWGNLLRDGFNVFIAELVYTLPFILLMVVGALTTGGAATL
                     AGGEDLTALLATGGTLLFICLVFLFAIAFLFLAPAILIQYAIKGELGATFRFGEVFAI
                     IRNNMADILVAFLVTLVAAFAVSIIISVLSIVPCLGWIAAVIIGLAVGPYISFVGGHL
                     YGQIAAKILGNKAGGTYTGAA"
     gene            902..1864
                     /gene="fmt"
                     /locus_tag="PLC06_13605"
     CDS             902..1864
                     /gene="fmt"
                     /locus_tag="PLC06_13605"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRO91529.1"
                     /translation="MIAVARIIFMGTPEFAVPCLTTLIETQTVVGVVTQPDKPAGRGN
                     RLRPSPVKVAAEAAGIPVYQPKSLRKEEAAAPLREWQPDLIVVAAFGQILRPHVLYLS
                     PRGSLNVHASLLPRWRGASPIQHAILAGDETTGVTLMKMDEGLDTGAMYVQESLSIDP
                     RETAATLHDRLAALGAAMLRRHLDDILDGRLSPTPQGDAGFTYAPMIRKEAGEIDWQR
                     DAGSLDRHIRAMTPWPGAFTWWGGEPLKVLGARPLDFSPSGVPGQVVFTPEGIIVVAG
                     SGGLLLDTIQAPGKRAMAAEEFVRGHPELAGAVLGPNPAAGLDE"
     gene            1872..2930
                     /locus_tag="PLC06_13610"
     CDS             1872..2930
                     /locus_tag="PLC06_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRO91530.1"
                     /translation="MNILVYGAGAVGGYLGGKLALAGRLVTLVTRPDTAAAINRDGLQ
                     LTENGNTRRALVRAVDSPVDALAGTAYDLIILGMKGYDLAEAVDQLAALCPEPARVMS
                     TQNGIGIEEIIAGRFGPGRTLAGSVTIPISRAGLNHLVVERTGRGLGLAPVAPGQSTA
                     EWVALFREAGINAGARDDYRAMKWSKAFLNIMGNATSAILNRPPAELYRLQSIFDLEM
                     RMLRETLAVMKRLGIEVINLPGATARPLARSLTYAPSFLLRQVFTQVIVRGRGDKMPS
                     FHIDLSGGKAQSEVIFHNGAIATAGQRAGVPAPVNAVLNDTLQSLCRDRSQRAWFDGR
                     PDRLLAVIREYEQGKVKV"
     gene            2927..3670
                     /locus_tag="PLC06_13615"
     CDS             2927..3670
                     /locus_tag="PLC06_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRO91531.1"
                     /translation="MSAAYAGHVQEGALRQEMVRIGQMMYAAGLLCGFEGNLSVRMSD
                     SRILITPAGLHKGLLRPEQLLVVDNEGRLIGYPTEARRGLRPTSELPMHMEAYRQRPD
                     IVAVVHAHPPITVALSIAGVPMNTPLLPEVIVLMGQIPMAPYSMSSSEEGASQIRELV
                     RHHDAIILQRHGTLTVGNSLTQAFMRLETVEQNARINFMLAQLGANGSMEGRELVKLL
                     KMRRGMGLERPGDAADYRRLWGVNADDPV"
     gene            complement(3667..>4175)
                     /locus_tag="PLC06_13620"
     CDS             complement(3667..>4175)
                     /locus_tag="PLC06_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO91532.1"
                     /translation="IWGLWANPPQETAVGTLTTLNFRKELWPWAVTAISDFPLTGVGL
                     GAFRLVAFRLYPLPLSPQFDIGHAHNVFLQTALDVGLPGLAVYLAILLVALVVGWQVA
                     RSDLGYRAISLGILAGLVAHHVYGLADTLALGSKPGLVFWLLLGLLAAAHRLTHVMTA
                     GTTPDGED"
BASE COUNT          840 a         1230 c         1324 g          781 t
ORIGIN      
        1 atcaggcagc gcccgtatag gtgccgccgg ccttgttgcc gaggatcttg gccgcgatct
       61 gaccgtatag atgcccaccg acaaacgaga tgtaggggcc gaccgccaag ccgatgatga
      121 cggcggcgat ccagcccagg caaggaacga tgctcaggac gctgatgatg atcgacacgg
      181 caaacgcggc gacgagcgtc acgaggaatg cgaccaggat gtccgccata ttattgcgga
      241 tgatggcaaa tacctcgccg aagcggaagg ttgccccaag ttcgccttta atggcatact
      301 ggatcaggat agccggtgcg aggaaaagaa aggcgattgc aaacaggaag accaggcaga
      361 taaacaagag cgtgccacct gtggccagta gagccgtcaa atcttcgcct ccggcgagag
      421 tagccgcccc ccctgtagtc agggccccga cgaccatcag gaggatgaaa ggcagggtat
      481 agaccagctc ggcgatgaac acattgaagc cgtcgcgcaa caggttgccc cagtcatccc
      541 actccggcag gccgtcccaa tcgccgttca tgacctggcg gataaccttg accacatacc
      601 cttgcagaat gaaggcggga aggatgaaga agctcaacac cgacatcaga gcgccaatca
      661 ccaccttgct gatccattgt ggatcatcga atacataccg aatcgacttg tttacatcca
      721 taatatttct ccttcgcttg atagctaatg ataattacga tgcatggtgg aaaaagttgc
      781 gcgaggattt gcattgtcgc gaaatgttgt ccaatcgcaa catgatgcat gatagtacga
      841 ttggttttgc ctgtcaacgg caatgacccg ggaatgtgca attaccccgc gccatgctag
      901 aatgatcgct gtggcaagga tcatctttat gggcacgccg gaattcgccg ttccgtgcct
      961 gacaaccctc attgaaaccc agaccgttgt cggcgttgtg actcagcccg acaagcctgc
     1021 cgggcggggg aatcgcttgc ggccgtcgcc ggtcaaggtc gcggccgaag cggccggtat
     1081 acccgtctat cagcccaaat cgctgcgcaa ggaagaggcg gccgctccgc tgcgtgagtg
     1141 gcagcccgat ctcatcgtcg tcgctgcctt cggtcaaata ttacgtcccc atgttctcta
     1201 cctgtcaccc agggggagcc tcaatgttca tgcctcgctg ctgcctcgtt ggcgcggcgc
     1261 gtcgcctatt cagcacgcca ttctggccgg cgacgaaacg accggcgtca cgctcatgaa
     1321 gatggacgaa gggctggata cgggggccat gtacgtgcag gaatcgttga gcattgaccc
     1381 gcgcgagacg gcggccacgc tccatgaccg attggcagcg ctgggcgcgg ctatgctgcg
     1441 tcgtcatctg gacgatattc ttgacggccg cctgtcgccg actccccagg gcgacgccgg
     1501 cttcacctac gcgccgatga tccgcaagga agcgggcgaa atcgactggc aacgcgatgc
     1561 cggctcgctg gatcgtcata tccgggccat gacaccctgg ccgggagcat tcacctggtg
     1621 gggcggcgag ccgctcaagg ttctgggcgc ccggccgttg gatttctctc cgtcgggcgt
     1681 cccggggcag gtcgtgttta ctcctgaggg cattattgtg gtggcaggat cgggtggcct
     1741 gctgctggac acgattcagg ccccgggcaa acgagctatg gccgccgaag agttcgttcg
     1801 cggtcatccc gaactggccg gcgctgtcct cggcccaaac cctgcggcag gattagacga
     1861 gtaaccccgg aatgaatatc ctggtttatg gagcgggggc cgtcggcggc tatttgggcg
     1921 gcaaactggc gttggccggg cggttggtca cgctggtgac gcgccccgac acggccgcgg
     1981 ctatcaatcg cgacggcttg caactgaccg agaacggcaa tacgcgccgg gcgctcgtcc
     2041 gcgcggtgga ctcacctgtc gacgcattgg ccggtactgc ttatgacctg attatattgg
     2101 gcatgaaggg gtatgatttg gccgaggcgg ttgatcaatt ggccgcgtta tgccccgaac
     2161 cggcgcgggt gatgagcacg cagaacggca tcggcatcga ggagatcatc gccggtcgct
     2221 ttgggccggg gcgaacgttg gccggcagcg tcaccattcc catcagtcgc gcgggcctta
     2281 atcatttggt tgtggagcgc acggggcgtg gcctgggtct tgcgccagtc gcacccggcc
     2341 aatcgacggc cgaatgggtg gctctcttcc gcgaggcagg gattaacgcc ggcgcgcggg
     2401 atgattatcg ggcgatgaaa tggtcgaagg cgttcctcaa catcatgggc aacgccacct
     2461 cagccatcct caatcggccg cccgctgagc tttatcgttt gcaatcgatt tttgatctgg
     2521 agatgcgcat gttgcgcgag actctggcgg tgatgaaacg gctgggtatc gaggtgatca
     2581 acctgcccgg cgcgacggct aggccgctgg cgcgctcgct gacctacgcg ccgtcattct
     2641 tgctgcggca agtcttcacc caggtcatcg tccgcggccg tggcgacaag atgccctcat
     2701 tccacatcga tctgagcggc ggcaaggcgc agagcgaggt gattttccac aatggagcga
     2761 ttgccacggc gggacaacgg gccggtgtgc cggctccggt gaatgcggtt ctaaatgaca
     2821 cgctccagag cctgtgccgc gaccgctcgc aacgggcctg gttcgatggc cgccccgacc
     2881 ggttgctggc cgttattcgc gaatatgaac aagggaaggt gaaggtatga gcgcagccta
     2941 tgccgggcat gtccaggaag gtgcgttgcg gcaggagatg gtccggatcg gccagatgat
     3001 gtatgccgcc ggtttattat gtggcttcga aggcaacctg tcggttcgca tgagcgatag
     3061 ccgtatcctc atcaccccgg cgggcctgca taaagggctt ttgcgacctg agcagctgct
     3121 tgtcgtcgac aacgagggac gtttgatcgg ctacccgacc gaggcgcgac gcggcctgcg
     3181 gccgaccagc gagctgccca tgcacatgga agcttaccgg cagcgcccgg atattgtcgc
     3241 cgtcgtccac gcccacccgc ccatcactgt ggcgttatct atcgccggtg tgcccatgaa
     3301 cacgccgctt ctgccggagg tgatcgtgtt aatgggccaa attccgatgg ctccctattc
     3361 catgtcttcc agcgaggaag gggcgagcca gattcgggag ctggtgcgcc atcacgacgc
     3421 catcatcctg caacgccatg gcacgctgac cgtgggtaac tccctgacgc aggcgttcat
     3481 gcgtctggag acggtggagc aaaatgcgcg catcaacttc atgctggctc aattgggcgc
     3541 gaacggctcg atggaaggac gagagctggt gaagctcctg aaaatgcggc gggggatggg
     3601 tctggagcgc cccggagacg cggccgatta caggcgattg tggggagtca acgccgacga
     3661 cccggtttag tcttcaccat ccggcgttgt gccggcggtc ataacgtgtg tcagtcgatg
     3721 cgcggctgcc aacaggccaa gcaataacca gaataccagg ccaggcttgg aaccgagggc
     3781 caacgtgtcg gccagcccat aaacgtgatg ggccaccaac cccgccaata tccccagact
     3841 gatggcgcga tagcctaagt cgctccgcgc aacctgccaa ccgacaacca gagccaccag
     3901 gagtatggcc aaatacaccg ccaagcccgg taggccgacg tcgagcgccg tttgcaggaa
     3961 cacgttgtgg gcgtggccga tgtcgaattg gggcgagagc ggcaatgggt agaggcggaa
     4021 ggctaccagc cggaatgcgc cgaggcccac accggttaag gggaagtcgg agatagccgt
     4081 caccgcccag ggccacagct ccttgcggaa gttcagcgtc gtcagcgtgc cgacggccgt
     4141 ttcctgtggc ggattggccc atagccccca gatgg
//
LOCUS       DAOMQG010000313         4152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8908_length_4152_cov_8.056871, whole genome shotgun sequence.
ACCESSION   DAOMQG010000313 DAOMQG010000000
VERSION     DAOMQG010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_4152_cov_8.056871"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..313
                     /gene="vapB"
                     /locus_tag="PLC06_13625"
     CDS             104..313
                     /gene="vapB"
                     /locus_tag="PLC06_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRO91533.1"
                     /translation="MDTARIFIHDNDQAICLPEGYRFEGTEVYIQKVGNVVVLSPVAG
                     SWQTLIDSLGQFSDDFMENREQPDV"
     gene            complement(375..2351)
                     /locus_tag="PLC06_13630"
     CDS             complement(375..2351)
                     /locus_tag="PLC06_13630"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HRO91534.1"
                     /translation="MRNDRSKLNIPYLVAAIALLALVVLASLYFFPEWRESLAMMIAL
                     LVVVTVGVVGFAANFTSAFIRDSVDTPSVALDYDSPQHRRNQENVCANVRSAWIDGVL
                     RDSLQERVRLELALDSPPGMLQRAIRPVEGQEPRRPLESTADIADIYRAGARSLLILG
                     APGSGKTMTLLELCRPILEGAEVDPRRPVPVVLNLSTWAQKQAPLAEWIADEMRRQYD
                     LSRAVTPVWLAADHMTLLLDGLDEVSADARDACVRAINAFRETHSAGMAICSRSADYA
                     DLRERLALSKAVEILPLTPSQIAAYLSDERLALDGVREAIARDDALGELATTPLMLNV
                     MAAAYGGRLLVELLPLLDSEIERRAHLYDAYIARMFSRKPLTGTGYTAAQALRWLRFL
                     AARLIERDETQFFIEDLQPDWMPRKGRYLGPILPVLKPVDEFPIILEPRLQLQIIMDR
                     WPLLGLIVVLGGYLGFQLGNKISYVLIGILVSIGVTLWIGFMTRMLLEWRGRTLSFFS
                     SVTYNGKSREKSRYYKSVGQGIRRSSQNAILIVAVGGIITVPLFFMFLGVLESVINST
                     GNFRIVVAVTIGLFYWVIIFGLTSGGLFVLMHYILRLLLALHHLLPLRLVPFLEAMRE
                     RILMQRAGGHYRFIHRTLQEHIAALTDDRIERLT"
     gene            complement(2531..3673)
                     /locus_tag="PLC06_13635"
     CDS             complement(2531..3673)
                     /locus_tag="PLC06_13635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO91535.1"
                     /translation="MSSINHDMIELARTLAADIAGRATTADREGRLPAADVALLRQSG
                     YLALAVPREFGGRGESLLVCTAAQLELAKGSASTALVAAMQHQVAGHQRETRTWRPDA
                     REQIMCAAAAGGLLNHLGSEPRLGSPSRGGLPDTVATPTPDGDRLLINGRKTWATGGR
                     HLTHMLVRVAVRQEGGVVLVRQGDEDTPGVEWIETWRDALSLRATDSHDVVFHDVAVP
                     RENLIETGNPRATPNVWFPMMLSAVYLGTALAARDALVTYALERVPTAIGKPIATLPK
                     IQRQVGEIDVALMAARALLFEVAGEWTGRDEDRLAMMGRIAAAKMAVSVAANEATEKA
                     LAAAGGAGLTGALPLERCFRDVRAAGMQPPSGDTAYEMVGRAALGV"
     gene            complement(3670..4035)
                     /locus_tag="PLC06_13640"
     CDS             complement(3670..4035)
                     /locus_tag="PLC06_13640"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRO91536.1"
                     /translation="MKQSESTHTPDAMQRQALRTWHNTTIPLREQRIHALLGLAGETG
                     ELVDLLKKHFYKPDREATQAMVMDELSDVLYYVAILSHLWEFTFDDMAAHLAVKLADS
                     HGWDGSGEKVAGQINGDAR"
BASE COUNT          770 a         1194 c         1387 g          801 t
ORIGIN      
        1 cattacgcaa cgcctttggc cccacgctct gccggatgat ctggtattgc aatgaatgcc
       61 gggacgcgtt cgagcagttt aagcctttat aaggtgcttt cttatggata ccgccaggat
      121 cttcatccac gacaacgatc aagcgatctg cctgccggag ggataccgtt tcgaggggac
      181 agaggtttat atccaaaagg tcggtaatgt cgtggttcta tcccctgttg ccggatcatg
      241 gcagacgctc attgacagtc tggggcaatt ctccgatgat tttatggaga atcgagaaca
      301 gccggacgtg tagtgggtga tgggtgattt cgccgctgca tcgccccacc cattttcatt
      361 cttgtcttgt gtgtctacgt aagacgctcg atgcggtcgt cggttagggc ggcgatgtgt
      421 tcctggaggg tgcggtggat gaagcggtag tggccgccgg cgcgctgcat gagtatgcgc
      481 tcgcgcatgg cttcgaggaa ggggacgagg cggaggggga ggaggtggtg gagagcgagg
      541 aggaggcgga ggatgtagtg cataaggacg aacaatcctc cagatgtcag tccaaaaatt
      601 attacccaat aaaatagtcc tattgtcacg gccacgacaa ttcgaaaatt gccggtggaa
      661 ttaataaccg attccaatac ccccaaaaac atgaagaaaa gcggcaccgt aatgatgcca
      721 cctactgcaa caattaaaat cgcattttga ctgctacggc gaattccttg gccaacactc
      781 ttgtaatacc tacttttttc acgactcttg ccgttgtatg tgacgcttga gaagaatgaa
      841 agggttcttc ctcgccattc gaggagcatc ctagtcataa agccgatcca tagagtaacc
      901 ccaatgctaa cgagtattcc aatcaacaca tagcttattt tattccccaa ttgaaagccc
      961 aaataccctc ctaacacaac tatcaagcct aataatggcc atctatccat gatgatctgt
     1021 aattgtaagc gtggctcgag gataattggg aactcgtcaa caggtttcaa tacaggtagt
     1081 attggcccca aatatcttcc cttacgaggc atccaatccg gctgcaaatc ctcaataaag
     1141 aactgcgtct cgtcccgctc gattagccgg gcggcgagga agcgcagcca acgcagcgcc
     1201 tgcgcggcgg tgtagcccgt gccggtcagc ggcttgcggc taaacatgcg ggcgatgtag
     1261 gcgtcgtaga gatgggcgcg gcgctcgatc tcgctgtcga gcaggggcag cagttcgacc
     1321 agcaggcggc cgccgtaagc cgcggccatg acgttgagca tcagcggtgt cgtagccaac
     1381 tcgcccaggg cgtcgtcgcg ggcgatggcc tcgcgcacgc cgtccagcgc cagccgttcg
     1441 tcgctcaggt aggcggcgat ctgggacggg gtcagaggca atatctcgac cgctttggac
     1501 agggccagcc gctcgcgcag atcggcataa tcggccgagc ggctgcagat ggccatgccc
     1561 gcgctgtggg tctcgcggaa ggcattgatg gcccgcacgc aggcgtcgcg ggcgtcggcg
     1621 gacacctcgt cgaggccgtc gagcagcagc gtcatatggt cggcggccag ccagacgggg
     1681 gtgacggctc ggctcaggtc atattgccgc cgcatctcgt cggcgatcca ctcggccagc
     1741 ggcgcttgct tctgcgccca ggtggagagg ttgaggacga cgggaacggg gcggcgggga
     1801 tcgacctcgg ccccctctag tattggccgg catagctcca gcagggtcat ggtcttgccc
     1861 gaaccgggcg cgccgaggat gagcagggag cgagcgccgg cgcggtagat gtcggcgatg
     1921 tcggctgtgc tctccaacgg gcggcggggt tcctgcccct cgacaggacg tatcgcccgc
     1981 tgcaacatgc ccggcgggct gtcgagggcc agctccagcc gcacgcgctc ctgtaaggag
     2041 tcgcgcagca cgccgtcgat ccacgcgctg cggacgttgg cgcagacgtt ctcctgattg
     2101 cgacggtgct gcggggagtc gtagtccagc gcgaccgatg gggtgtcaac actatcgcga
     2161 atgaaggcgg aggtgaaatt ggcggcaaac ccgaccacgc cgaccgtcac gacgacaagc
     2221 agtgcaatca tcatcgccag cgattcgcgc cattcgggga agaaatacag gctggcaagg
     2281 acgacaaggg ccagcagggc gatggctgct accagatagg ggatgttgag tttgctgcgg
     2341 tcgttgcgca tcggttcacc ccacgcttgg aagagaaagc tgcctatatt aatgcgacag
     2401 gggggatgat agcgcgaatg atggcggcgg gcgaagaagg gcgaggcagc ggcgaacgaa
     2461 gttggttctg cgtggcgtag gtggtttcgt cgctgccatg tccctatgac cgacatcttt
     2521 attttcggga ctacactccc aacgccgccc tccccaccat ttcataggcc gtatcgcccg
     2581 atggtggctg cattccggcg gcgcggacgt cgcggaagca acgctccagc ggcagcgctc
     2641 cggtgaggcc ggccccaccg gccgccgcca gcgctttctc ggtcgcctcg ttggccgcga
     2701 ccgacacagc catcttggcc gcggcgatgc ggcccatcat cgccagccgg tcttcgtcgc
     2761 gccccgtcca ctccccggcc acctcgaaca gcagcgctcg cgccgccatg agggccacgt
     2821 cgatctcccc cacctgacgc tgaatcttgg gcaatgtggc gatgggtttg ccgatggcgg
     2881 tcggtacgcg ctctagcgca taggtcacca gcgcgtcgcg ggcggccagc gctgtgccca
     2941 gatagacggc cgagagcatc atcgggaacc agacgttagg ggtggcgcgg gggttaccgg
     3001 tttcgatgag gttttcgcgc ggcacggcca catcgtggaa gacgacatca tggctgtctg
     3061 tggcccgcag gctgagggcg tcacgccagg tctcgatcca ttcgacgccg ggcgtatctt
     3121 catcgccctg gcgaacgagg acgaccccgc cctcttggcg aacggccacg cgcacgagca
     3181 tgtgagtcag atggcggccg ccggtggccc aggtcttgcg gccgttgatg agcaggcggt
     3241 cgccgtcagg cgtgggggtg gcgacggtgt ccggcaggcc gccgcgtgag gggctgccga
     3301 ggcgcggctc actgcccagg tggttgagca ggccgcccgc ggcggcggcg cacatgattt
     3361 gctcacgggc gtcgggtcgc caggtgcgcg tttcgcgctg gtggccggcg acctggtgct
     3421 gcatggcggc gacgagcgcg gtggaggcgc tgcccttggc cagctccagt tgggcggcgg
     3481 tgcagacgag cagcgattcg ccacggccgc caaattctcg cgggacggca agggccagat
     3541 agcccgattg acgtagcagg gcgacgtcgg cggcgggcag gcggccttcg cggtcggcgg
     3601 tggtggcgcg accggcgatg tcggcggcca gggttcgcgc cagttcaatc atatcgtggt
     3661 tgatgctgct catcttgcgt cgccgtttat ctgcccggcg accttctcgc cgctgccgtc
     3721 ccagccatga ctgtcggcca gcttgacggc cagatgggcg gccatgtcgt cgaaggtgaa
     3781 ctcccacagg tgggagagga tggcgacgta gtagagcacg tcggacagtt cgtccatgac
     3841 catcgcttgt gtcgcttcgc ggtcgggctt atagaaatgc tttttgagca agtcgaccag
     3901 ttcgccggtc tcgccggcca ggccgagcag ggcgtggatg cgctgctcgc gaagggggat
     3961 ggtggtgttg tgccatgtgc gcaatgcttg ccgctgcatg gcgtcggggg tgtgggtgga
     4021 ttccgattgt ttcatatgat ggattttact agatcggttt gttgtgggca gcccagtaag
     4081 cgccggccca ggtgacggcg cgatggatgc cttcacgcag cggcacctgg ggggtgtagc
     4141 cgaggagggc gc
//
LOCUS       DAOMQG010000314         4152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8909_length_4152_cov_7.702465, whole genome shotgun sequence.
ACCESSION   DAOMQG010000314 DAOMQG010000000
VERSION     DAOMQG010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8909_length_4152_cov_7.702465"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLC06_13645"
     CDS             complement(<1..450)
                     /locus_tag="PLC06_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91537.1"
                     /translation="MSRKREVLLVAGLFVIAVIGSAMVWSLLPAEYRENQSTDYLNAY
                     EPVARAIAAGQGITLDGEIATRYPPGFSILLAGVFRSGSTLNVADETALLAFRLVCVG
                     LAVVLVYGLARLVWSPRAALIPALAWMTYPFSLWLAKQPNSEVPFVPV"
     gene            complement(447..1847)
                     /locus_tag="PLC06_13650"
     CDS             complement(447..1847)
                     /locus_tag="PLC06_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRO91538.1"
                     /translation="MPDWLRIYHKLPYPAKMLAAGARGRYLNRWRYSAATEPMVAEYL
                     AHDGWSDEQWRAWREERLAFVLRRAAEDVPYYREQWARRRAAGDRASVEYLENWPVLG
                     KEALRAAPRAFLADDCDPGSMFYEHTSGTTGKPLHIWLSRETVQAWFALFEARARRWY
                     GVDRHDRWAILGGQLVAPVERDRPPFWVWNMGLNQLYLSTYHLAPENIAAYVAAIEKH
                     EIRYLLGYPSALYRLARELLDQGLAGPRLQVAIGNAEPLLDHQREAITAAFDCPARET
                     YGMAEIAAAAGDCELGRLHVWPEAGYIEIFADTADEVVPVGQSGRLVATGLMNADMPL
                     IRYETGDRATMAVEGCACGRALPVLQAIEGRVDDVVVTRDGRHIGRLDPVFKADLPIV
                     EAQIIQESLDTLRVLYVPTATYTEADGTSLVQRIHDRTGDDMRVILEAVEAVPRTANG
                     KFRAVISRVGREGKAP"
     gene            complement(1840..2952)
                     /locus_tag="PLC06_13655"
     CDS             complement(1840..2952)
                     /locus_tag="PLC06_13655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91539.1"
                     /translation="MAIINDSPAGVPEVERPAEAVEAATRPRLCFVGPMLGVNPGWVS
                     TQGELLAGLLAEAGYPTRLTSHKPARLPRLVDTLSSLVAWRNEIDLVIHQVFSGPAFA
                     ITDAATALGRALGLRQIAVLHGGALPEFADRHGGWVRRVLGRMAAVVAPSTYLAETFA
                     VYPELAPRLRVIPNILEIDNYPYRHRASVRPNLLWMRTFHEVYHPEMAVEVLAELRQT
                     HPAATLTMAGQEKGLHAVVQELARRRGLVDAVYFPGFLNPEGKLREFATHDIYLNTNR
                     VDNMPVSVLEAAAFGLPVVATAVGGVPHLLRDGETGLLVPDGDVVAMAAAVRRLLGEP
                     GLAATLSANGRALAESCAWEPVRRQWESLFAEVLHA"
     gene            complement(2939..>4152)
                     /locus_tag="PLC06_13660"
     CDS             complement(2939..>4152)
                     /locus_tag="PLC06_13660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91540.1"
                     /translation="PHYHAGDQIVGWGPTVRELGYLAALFDSVVHVAVGYPGPAPASA
                     LPYAAANVRVRLVEPAGGDTLRDKIGILTAYPNYARVIREEMGRADAIHVRCPANISL
                     LALWQLGRRELPPYRWVKYAGNWRPDGGEPRSYRLQRRWLTENRHHGVVTVNGRWPNQ
                     PNHVVSFYNPCLTDEEAAQGGTLAAGKMLGSPVELLFVGELNEGKGVGRVLRVALALQ
                     NRGIPFRLRLLGDGPDRPRYEAWVRENKLAGTSFLGWVPRTQVADYYAAAHFILLPSR
                     SEGWPKVLSEAMAYGVVPVAAAVSSIPQILGETRAGVALPVDDVEGMAEAIAGYVTEP
                     ERWAAAGRAGVVAAHRFTYGAYQSAVAGLFSGAWGVHLSIPEKPVGHEPVLIPAQPDP
                     VNVPYSSNGYH"
BASE COUNT          834 a         1451 c         1243 g          624 t
ORIGIN      
        1 cacaggcaca aacggcacct cgctattggg ctgcttggcc agccacaacg aaaatgggta
       61 cgtcatccag gccagagcgg ggatcagggc cgcgcgggga gaccagacca accgcgccag
      121 cccatagacc agcacgacgg ccagtccgac gcacaccagc cgaaaagcca acaacgctgt
      181 ctcgtcggcc acattcagcg tgcttcccga tcgaaaaacc ccggccagca aaatcgaaaa
      241 acccggcggg tagcgcgtgg cgatctcgcc gtcaagtgtg atcccttgcc cggcggcgat
      301 agcccgcgcc accggctcat aggcattgag gtagtcggtg ctctggtttt cccggtattc
      361 ggccggcagg agcgaccaga ccatggccga accgatcacc gcaatgacaa acagccccgc
      421 gaccagcaga acctcgcgct tccggctcat ggcgccttcc cttcgcggcc gacgcgcgag
      481 atgacggcac gaaacttgcc gttggccgtg cgcggcacag cctcgaccgc ttccaggatg
      541 acgcgcatgt cgtcgccggt tcggtcgtgg atgcgctgca ccagcgacgt gccatccgct
      601 tcggtatacg tcgcggtggg cacgtagagc acccgcaacg tgtccaggct ctcttgaatg
      661 atctgggcct cgacgatggg caagtcggcc ttgaacaccg gatcgaggcg gccgatgtga
      721 cggccgtcac gcgtcacaac gacatcatcg acgcgccctt caatagcttg caggacgggc
      781 agcgcgcggc cgcaagcgca cccttcgaca gccatcgttg cccgatcccc cgtctcgtag
      841 cgtataagcg gcatgtcggc attcatcaag ccggtagcga cgagtcgccc cgactgccca
      901 accggaacga cttcatcggc cgtatcggca aaaatctcga tgtagcccgc ctccggccat
      961 acatggagtc gccccagctc gcaatcgccg gctgcggcgg cgatctcggc catgccgtag
     1021 gtctcgcggg ccgggcaatc gaaagccgcg gtgatagcct cccgctggtg atcgagcagt
     1081 ggttcggcat tgccgatagc gacctgcagc cgtggcccgg ccagcccttg gtcgaggagt
     1141 tcccgtgcca atcgatagag agccgacgga tagcccagca gatagcggat ctcgtgcttc
     1201 tcaatcgcgg ccacataggc agcgatgttc tccggcgcga gatgataggt cgagagatag
     1261 agctgattga ggcccatgtt ccacacccag aacggcggtc gatcgcgctc aaccggcgcg
     1321 acaagctgac caccgaggat cgcccagcga tcatggcgat ccactccata ccagcgtcgc
     1381 gctcgcgcct cgaacagcgc gaaccaggcc tgcaccgtct cccggctcaa ccagatgtga
     1441 agcggcttgc ctgtcgtacc gctggtgtgc tcatagaaca tcgacccggg atcgcaatcg
     1501 tcggccagga aagcgcgcgg cgcggcccga agggcttcct tgcccagcac cggccagttc
     1561 tccaaatact cgacagaggc tcgatctccg gccgcccgcc gccgcgccca ttgctcccga
     1621 taatagggca catcctcggc cgcccgacgc aggacaaaag ccaatcgctc ctcgcgccac
     1681 gcccgccact gttcgtcgct ccacccgtca tgagcgaggt actcggccac catcggctcg
     1741 gtggcggcgc tgtagcgcca gcggttcaga tagcgaccac gggcgccggc ggccagcatc
     1801 ttggccggat agggcagctt atgataaatg cgcaaccagt caggcatgca gcacctcagc
     1861 gaacaggctt tcccactgtc gcctaaccgg ctcccacgcg cacgattccg ccagcgcgcg
     1921 gccgttggcc gataatgtcg cggccaatcc cggctcaccg agcaagcgcc gaaccgcggc
     1981 ggccatcgcc accacatcac catcaggcac cagcaacccg gtctcgccat cacgcagcag
     2041 gtgaggcacg ccgccgacgg ccgtggccac caccggcagc ccgaacgccg ccgcctccag
     2101 cacactaacc ggcatattgt caacgcgatt ggtgttgaga tagatgtcgt gggtggcgaa
     2161 ctcccgcaat ttgccctccg ggttcaaaaa accgggaaag tacacggcat cgactaaacc
     2221 gcgccgtcgc gccagctctt gcacgaccgc gtgcaacccc ttttcctgcc cggccatcgt
     2281 cagcgtcgcc gccgggtgcg tttgacgcag ctcggccagc acctcgaccg ccatctccgg
     2341 atgatagact tcgtgaaaag tgcgcatcca caacaagttg ggcctgacgc tcgctcggtg
     2401 gcgatagggg tagttgtcga tctccaggat attggggata acgcgcaggc gaggagccag
     2461 ttcgggatac acggcaaacg tttccgccag atacgtagat ggggcgacga cggccgccat
     2521 gcgacccagg acgcgcctca cccagccgcc gtgtcgatcg gcgaactccg gcagcgcccc
     2581 gccgtggagc acggcgatct gccgcaggcc caacgcgcgg ccgagcgccg tggccgcgtc
     2641 ggtgatggca aaagccgggc cgctgaacac ctgatggatg accaggtcga tctcgttgcg
     2701 ccaggcgacg agcgatgaaa gcgtgtcgac cagccgtggc agtcgcgccg gtttatgcga
     2761 ggtgagtcgc gtcgggtagc cggcctcggc caacaacccg gccagcagct ctccctgcgt
     2821 cgacacccaa ccgggattga cgcccagcat cggcccaacg aaacacaggc gcggacgggt
     2881 ggcggcctcg accgcttcag cagggcgctc aacttccgga acgccggcag gtgagtcgtt
     2941 aatgatagcc attgctcgaa taaggaacgt tcaccggatc aggttgggcg gggatcaaaa
     3001 ccggctcgtg gccaacaggc ttctccggga tagacagatg aacaccccac gccccggaga
     3061 acaaaccggc gacggccgac tgataagcgc cgtaggtgaa gcgatgcgcc gccacgacgc
     3121 ccgctcgccc ggcggccgcc caccgctccg gttctgtgac atagccggcg atcgcctcag
     3181 ccatcccctc gacgtcatcc accggcaagg ccaccccggc ccgcgtctcg cccagaattt
     3241 gcgggatgct ggaaacggcg gcagccaccg gcaccacacc gtaggccata gcctcgctca
     3301 atactttcgg ccaaccctcg gagcgggaag gcaacaggat aaaatgggcc gcagcataat
     3361 aatcggccac ctgcgtgcgc ggcacccacc ccaggaacga cgtcccggcc agcttgttct
     3421 ctcgcaccca ggcctcatag cgcggccgat ccgggccgtc acccagcagg cgcaagcgaa
     3481 acgggatacc ccggttttgc aaagccagcg ccacccgcag aacgcggccg acgcccttgc
     3541 cttcgttgag ttcacccacg aagagcaact ccaccggcga cccaagcatc ttgcccgcgg
     3601 ccaatgtgcc accttgggcc gcctcttcgt cggtcagaca cgggttatag aaggatacga
     3661 catggttggg ctgattgggc caacggccgt tgaccgttac cacgccatga tgacgattct
     3721 cggtcagcca acgtcgctgc aatcggtagg agcgaggctc gccgccgtcc ggccgccagt
     3781 tacccgcgta cttgacccac cggtagggtg gcagctcgcg ccgccccaac tgccataacg
     3841 ccaacaaact aatattggcc gggcaacgaa cgtgaatcgc gtccgcacgg cccatctcct
     3901 cgcgaatgac gcgggcatag ttgggatacg cggtcaggat accgatcttg tcccgcagcg
     3961 tgtcgccgcc ggcaggttcg accaaccgaa cccgcacgtt ggccgccgcg tagggcaacg
     4021 cgctcgccgg ggccggacca ggatagccga cggcgacatg aacgacgcta tcgaacaacg
     4081 ccgccaggta gcccagctcg cgcaccgtcg gaccccaacc aacaatttgg tcaccggcat
     4141 gataatgggg gg
//
LOCUS       DAOMQG010000315         4142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8939_length_4142_cov_6.422804, whole genome shotgun sequence.
ACCESSION   DAOMQG010000315 DAOMQG010000000
VERSION     DAOMQG010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8939_length_4142_cov_6.422804"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..765
                     /locus_tag="PLC06_13665"
     CDS             481..765
                     /locus_tag="PLC06_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91541.1"
                     /translation="MADMTNRQQQLEALFQEAGTAHHTAFIEVDGADADWPIWYAEYL
                     RDRLAELLGAQFTRSELVYLLVGLDREVQRVAPGANWQAYYARALLERYG"
     gene            790..1434
                     /locus_tag="PLC06_13670"
     CDS             790..1434
                     /locus_tag="PLC06_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRO91542.1"
                     /translation="MFNQRERRELEQRMREQDRLPPGQSATLKFPVLHYGPVPIVDLA
                     TWDFKVFGLVQEERRWNWDEFNRLPRTRVTLDIHCVTRWSKFDTLWEGVAVATLIEEG
                     LIQLKSEAKYVIQHCEYGYTTNTPVEFVLQPGFILATHYDGRPLSPDHGYPLRGVAGT
                     IRGQRADDKYFWKGGKWLRALEFRANDMPGFWERNGYHNQADVWREQRLGRPSF"
     gene            complement(1438..2028)
                     /locus_tag="PLC06_13675"
     CDS             complement(1438..2028)
                     /locus_tag="PLC06_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91543.1"
                     /translation="MASEQDILKHDLAILQSMAAGMGEYLRSDATWWDMGHRDMPLLT
                     IGGYLMRRRRLGVVEYLLHGPEREALAAANDHFDEVVGGGIVRFEERALAEIGARMRE
                     WTVYLRDLSVSQRLAADTARYEYQVGTRVVISDLLEKLGESSFRLPGHISDDVTALDH
                     RLGARWTPGAFIWSPVWTPAYLPDTHWWLYGHPKAH"
     gene            complement(2177..2572)
                     /locus_tag="PLC06_13680"
     CDS             complement(2177..2572)
                     /locus_tag="PLC06_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HRO91544.1"
                     /translation="MTTWTKDELNRVGTAEELEIASLRQDLTLRKPRTIWVVRVEDNL
                     YVRSVRGRGSDWFRGVQTRHEGHIQAGGVEKDVTFVEVDADDDINDRIDAAYRAKYRR
                     YAENIINSTLTSEARSATVRLIPRSPGLA"
     gene            complement(2652..3806)
                     /locus_tag="PLC06_13685"
     CDS             complement(2652..3806)
                     /locus_tag="PLC06_13685"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HRO91545.1"
                     /translation="MPVPSVHTGPEQEHEVTPLELFFDLVFVFGMSQLSHHLRIHLSW
                     HGAAETLVMLIAIFLVWFRTSWSVTMIPADRSRTLWMILTVMLLGLFMNASVTSAFTI
                     SGYAFVIPYLLIQLGRTLWTYFNAPNAVYREHYFRVLIWLIPTIPLWVAGAAADSDVR
                     LLWWGLAAGIDLLGTWLAHPLPGRRLHSENVGFDTNHMLERCRLFLLIALGETIIETG
                     TAIAEVSMTLMTLVTGTAALVGTVALWALNFGHSHRPAMPYREKAYDPIRISRYAVNA
                     LIGLFAGLIAVAVGNELVITHPEGATSLSLSLLLYGGSILYLLAQRWYLRAALGDLTR
                     LRFIGSIALAAVGLFTLTTPPYISLILLSVSLTILAIFDQRTTGQPGSVA"
BASE COUNT          965 a         1193 c         1141 g          843 t
ORIGIN      
        1 cgctccctcc ggcgactgaa cgccacagtt cgggcaagtt atcgtgtcca taaactcctt
       61 ccttccagat ccatcgccgg gtgcctgtct ccggatgaga tctgtcgcca ttataccgca
      121 gactcagatg agttcgaccc aactcatggg gatttcagtt catagtgcac acccttgcaa
      181 ttggagcgtc aacaatgatg gcaaacgatc tcattcatga acttccttta cgtacgtttg
      241 gagcgggcag ttgaattgac ggaagacgaa agaggttggc ccccttcgac tctataatag
      301 cgaatatagc ccacattgta gctctgcttc gaagcgcctg acaaaataac cgaactgtgt
      361 agcgagcctg actcgaaaac aagcggagaa accattagta acagtgtagt gggtttaccg
      421 cgcaggcttc atgtcgtaat atcatgacac gattggaaat tctgcaacag gtgagagaac
      481 atggcggaca tgacaaacag acaacaacaa ctcgaagcgc tattccagga agcggggacc
      541 gcccatcata cggccttcat tgaggtagac ggggccgacg ccgactggcc catctggtat
      601 gccgaatacc tgcgagatcg tctggcggag ctgctcggcg cgcagttcac caggagcgaa
      661 ttggtctatc tgctcgtcgg gctggaccgc gaggtgcagc gcgtggctcc cggcgcgaac
      721 tggcaggcct attatgcccg cgcgctactg gagcgctacg gctgagccac ggtcgtcaag
      781 agggacgtca tgtttaacca acgagaacgg cgcgagctgg agcaacggat gcgggaacag
      841 gatcggttgc cgcccggtca atcggcgacg cttaagttcc ccgtcctgca ttacgggcct
      901 gttcccatcg tcgatctggc gacctgggat ttcaaagtgt tcggcctggt tcaggaagag
      961 cgtcgctgga actgggacga attcaaccga ttgccgcgca ccagagtgac gctggatatc
     1021 cattgcgtca cacgctggtc gaagttcgat accctgtggg aaggtgtggc ggtggctacg
     1081 ttgatcgaag aggggctgat ccaactaaaa tcggaggcga aatacgtcat ccaacattgc
     1141 gagtatggat ataccaccaa cactcccgtc gaattcgtcc tgcagccggg atttatcctg
     1201 gccacccatt acgatggccg tcccctctcg cccgaccacg gctacccgct gcgcggcgtg
     1261 gccgggacca ttcgggggca gcgcgccgac gacaaatatt tctggaaagg cggaaaatgg
     1321 ctgcgcgctc tggaatttcg cgccaatgac atgcccggct tctgggaacg aaacggctat
     1381 cacaatcagg ccgacgtctg gcgcgagcag cgacttggcc ggccgagttt ctgatcgtta
     1441 gtgcgctttc gggtggccat agagccacca atgcgtgtca ggtaaataag cgggcgtcca
     1501 gacgggcgac cagatgaacg cgccgggcgt ccagcgcgcg ccgaggcgat ggtcgagcgc
     1561 cgtaacatcg tcggagatgt gccccggcag ccggaaggaa gattcaccca gcttctccag
     1621 cagatcgctg atcacgacac gcgtgccgac ttgatactca tatcgcgccg tgtcggccgc
     1681 cagacgctgc gataccgaca aatcgcgcag gtagaccgtc cactcgcgca tgcgcgcgcc
     1741 gatctccgcc agcgcccgct cctcgaaccg gacgattccg ccgcccacca cctcgtcgaa
     1801 gtggtcgttg gccgcggcca gggcctcgcg ctccggcccg tgcaacaggt actcgaccac
     1861 gcccagccgg cggcggcgca tcagatagcc gccaatcgtc agcagcggca tatcccgatg
     1921 gcccatgtcc caccaggtgg catcgctccg cagatactcg cccatgccgg cggccatcga
     1981 ttgcaggatg gccaaatcgt gcttcaaaat atcctgttca cttgccatgc gttaccccgg
     2041 taagttttcg accattgctg atcattaacc aattagtcta cttttgatta gtactgaaaa
     2101 taggctttac cctgtagctg tttcatttga tctatcctcg cttgccggcg ttgataaaga
     2161 tcgcactttc ccggtgttat gcaagacctg gtgagcgcgg tatcagcctg acggtcgcgg
     2221 aacgcgcctc cgaggttagg gtactattga tgatgttttc ggcatagcgg cggtatttag
     2281 cgcggtacgc ggcatcaatc cggtcattga tgtcgtcgtc agcatcgacc tccacaaagg
     2341 tgacatcttt ctcgacaccg ccggcctgaa tgtgaccttc gtggcgcgtc tggacaccac
     2401 ggaaccagtc ggagccgcgc cctctaacgg atcgaacgta gaggttatcc tcaacccgga
     2461 cgacccagat cgttctcggc tttcgcagtg taaggtcttg ccggagtgat gcgatctcca
     2521 attcctctgc tgtcccaacc ctgttaagtt cgtctttcgt ccatgtcgtc atcggttcgc
     2581 ctcctttata atcctggttg cgatatttta gcctgcttca tcgtctgcga ttgtaataga
     2641 cccaacttga cttatgcaac actgccaggc tgcccggtcg tccgctggtc gaagatagcc
     2701 aggatggtca ggcttacaga gagcaggatt aaggaaatat agggcggtgt agtcaatgtg
     2761 aagagtccga ccgctgccag agcgatactg ccgatgaaac ggagacgtgt taagtcgcca
     2821 agcgcggctc gtagatacca tctctgagca agcaagtaga gaatcgaacc gccgtagagc
     2881 agcaaactca atgaaaggga agtcgctccc tccggatggg taatcaccag ctcattccca
     2941 accgccacag cgatgagtcc cgcgaacaag ccgatgagag cgttcaccgc gtagcgactg
     3001 atgcgaatgg gatcgtacgc tttctcccga tacggcatgg cagggcgatg cgagtgcccg
     3061 aagttcagcg cccatagcgc gacggttcct actaacgcgg ctgtcccggt gaccagcgtc
     3121 atgagcgtca tagaaacctc tgcaatggcc gtacccgtct cgattattgt ttcacccaat
     3181 gcgatcagca ggaacagacg acaccgttcc agcatgtgat ttgtgtcaaa accgacgttc
     3241 tcggaatgga gacgccggcc aggaagggga tgtgccagcc atgtgcccag cagatcgatc
     3301 ccggcagcca gcccccacca cagcaggcgg acatccgagt cagctgcggc acctgcaacc
     3361 cataagggga tcgtaggaat tagccagata aggacccgga agtaatgctc tcggtagacg
     3421 gcattgggcg cattgaaata tgtccagagg gttcgcccca gttgaatcag cagataggga
     3481 ataacaaatg catagcctga gatggtgaat gcgcttgtca ctgaagcgtt catgaacaag
     3541 cccaacagca tcacggttaa tatcatccag agagtccgtg atcgatcggc cgggatcatg
     3601 gtgacagacc agctggtgcg aaaccacacc aggaatatag ctatgagcat caccagtgtc
     3661 tcggcagctc catgccatga tagatggatc cgcagatgat gcgagagctg ggacatacca
     3721 aacacgaata cgaggtcaaa gaacaactct aacggtgtaa cctcatgttc ttgctcaggg
     3781 ccggtgtgaa cggatggtac aggcatatat cctcgactat caccgtgatc aatcgtaaac
     3841 aggaagcctc gttcggaaac aataccgcca ccctcgttcg acggcaaatc ttacggccca
     3901 atcgctccag gctgctgacc gttcgcaaca atcgacgata tccggccggg cgaaaacgat
     3961 caacccctca gggatattac cctctatcca gccggccgga cgatgattaa caacccgggt
     4021 gcttaccgac atggatgcat gtacgattaa caaaacaacc gcgccggccg ccaaatagcc
     4081 tcacaacagg attgacgagg caattgattc tgctatagtg cccaaccatg tggcgctttc
     4141 ga
//
LOCUS       DAOMQG010000316         4123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_8988_length_4123_cov_9.601524, whole genome shotgun sequence.
ACCESSION   DAOMQG010000316 DAOMQG010000000
VERSION     DAOMQG010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8988_length_4123_cov_9.601524"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..85
                     /locus_tag="PLC06_13690"
     tRNA            10..85
                     /locus_tag="PLC06_13690"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:His,seq:gtg)
     gene            119..192
                     /locus_tag="PLC06_13695"
     tRNA            119..192
                     /locus_tag="PLC06_13695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Arg,seq:tcg)
     gene            complement(258..3245)
                     /locus_tag="PLC06_13700"
     CDS             complement(258..3245)
                     /locus_tag="PLC06_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRO91546.1"
                     /translation="MSVTLHINGQDQELHANTQDNLRDTLRDAGYMSVRFGSHSGETG
                     AAAVLVDGRLISTDAMVVGQVVGHRIETIDSLNSRVGELHPLQQAFIETGAIQSGYST
                     PAMILAAKGLLDRNPNPTEAEVRDALSGVLCRETGYLRPVEAILRAAAILRGEEPPPL
                     SGAVIVDATYMVENPEYRSPDGEEDDPNFAGPELSGGAPGPMTAIDTRPRTSIVLQGH
                     TPETSVVGKPETKVDAVKLAKGNPAFVDDIEMRGMLYARLLTSPHAHARILDIDDSEA
                     RALPGVRAVVHYKNVERVKYASGGQSYPNPPPHDQVSFDNKVRHVGDRVAAVAADTDE
                     IAEAALKLIKVEYEVLPAVFDEREAILPGAPVIHDEPDTEDIHDASRNIVHHIFAEKG
                     DVDGGFVQADRVFEHTYYVHQVQQVPIEPHIAISWWDADERMVIRTSTQVPFHVRRMV
                     APLLGLPVSRIRVIKPRIGGGFGAKQEMLIEDIVGHLTIATGRPVRLELNRSEEFHSS
                     RSRHPETITYKTGVMADGTLHSMEMKVLGNTGAYGTHGLTVLTVSGLRGLSSYNCEHR
                     RFDCKVVYTNIPTPGAYRGYGAPQALFALESHMDEIAHGLGIDPVDFRRKNWVQAGDT
                     LPMAVLLGEGEKNTVVDAPVILSCGLDECFVQGLAAIDWERRSNPDWHTVPGKPHVRR
                     GLGAAMCMHGTAIPGLDMGGAHIKINDDGSFNLTTGATDLGTGSDTILAQMAAEVLGV
                     PLSDIIIYSSDTDMTPFDTGAYASSTTYISGTAVKRAAELIADQIRERAALMLGLKEW
                     HNIRLENRQAIAPNGESVSMERIALHSLHQHDQRQLMASASFVSSASPPPFAGQFVEV
                     EVDTETGQVTVTKLVMAVDAGVPINPITASGQVEGGMVQALGYGHCEEMVYDADGRMV
                     NDALGPYHIYRADEMPELKAILVQTHEPTGPFGAKALAEIPKDGVAPAIANAVFDATG
                     VRLRRIPFTPQRVYEALQNQQEI"
     gene            complement(3282..>4123)
                     /locus_tag="PLC06_13705"
     CDS             complement(3282..>4123)
                     /locus_tag="PLC06_13705"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRO91547.1"
                     /translation="GFTSYYKQHADELPEALRHVANMKEYNQGAGRYLAHIYQARHAL
                     SIPIIGSLNGYYSSGWTRYARLIEAAGADALELNIYYLPTKAHITADEVEQMYIDLVR
                     DVTASVRIPVSVKLSPYFSAMANFAHRLVGAGAHGLVLFNRFYQPDFDIVNRAIIPSL
                     DLSTSSELRLRLRWVALLAQHIKADLAITGGVHTVEDVVKGLMAGANVVMMTSVLIQN
                     GIDYLKTLTDGLAGWLDANAYHSVSEIRGLLSHAQIGDSAALERANYLNVLRTAATKD
                     ETK"
BASE COUNT          791 a         1223 c         1209 g          900 t
ORIGIN      
        1 tcaggtatgg cgggtgtggt gtaggggtta acacgtctgg ttgtggccca gaagatcgtg
       61 ggttcaaatc ccaccactcg ccccaaaagc tgatggcgag atccatcagc tttttaatgc
      121 gcccgtagct caattggaca gagtatctgg cttcgaacca ggtggttggg ggttcgagtc
      181 cctccgggcg cacttcgtca tccgaacaag tggattggaa tgagcaggaa cggctttatt
      241 cgttcctgct catttcttta tatttcctgc tgattttgca gtgcctcata cacccgttgt
      301 ggggtgaagg ggatacgacg caggcgcacg cctgttgcgt cgaacaccgc gttggcgatg
      361 gctggagcga cgccgtcctt ggggatttcg gcgagcgctt tggctccgaa cgggccggtc
      421 ggctcatggg tctgaaccag gattgccttc agttcgggca tttcatcggc gcgatagatg
      481 tgatacggcc ccagcgcgtc gttgaccatg cggccgtcgg catcatatac catctcctcg
      541 caatggccat accccagcgc ctgcaccatc ccgccttcaa cctgccccga cgctgtgatg
      601 gggttaatcg gcacgcctgc atcgacagcc atcaccagtt tcgttactgt gacctgcccg
      661 gtctcggtgt cgacttcaac ctcgacgaac tggccggcga acggaggcgg cgaggccgag
      721 ctgacgaagg aggctgaggc catcaattgt cgctggtcat gctggtggag cgaatgaagg
      781 gcgatccttt ccatgctgac ggattcgcca ttgggggcga tcgcctgacg gttctccagt
      841 cggatgttat gccattcctt caaacccaac atcagggccg cccgctcgcg aatctggtcg
      901 gcgattagct cggccgcgcg cttcacggct gtgcccgaaa tataggtggt tgaactggca
      961 tacgcgccgg tgtcgaaggg ggtcatgtcc gtatcgctgg agtagatgat aatatcagac
     1021 agaggcacgc ccaatacttc ggccgccatc tgggccagga tggtatccga tccggtgccc
     1081 aaatccgtgg cgccggtggt caggttgaac gacccgtcgt cgttgatctt gatatgcgcg
     1141 ccccccatgt caagacccgg tatggccgtt ccatgcatgc acatggccgc tcccaggccc
     1201 cttcggacat ggggcttgcc gggaacagta tgccagtcgg ggttggatcg tcgctcccag
     1261 tcaatcgccg ccaggccttg cacaaaacat tcgtccaggc cgcaggacag gatgaccggc
     1321 gcatcaacaa ccgtgttttt ctcgccttcg cccaacagga cggccatcgg taacgtgtcc
     1381 cctgcctgaa cccagttctt tcgtcggaaa tcaaccggat ctatccccag cccgtgggcg
     1441 atttcgtcca tgtgggattc cagagcgaat aaagcctgtg gagcgccata gccacggtaa
     1501 gcgccggggg tggggatatt cgtgtatacc accttgcaat cgaaccgacg atgctcgcag
     1561 ttatagctgc tcaggccgcg caaaccggat actgtcagga cggtcaggcc atgagtgcca
     1621 taggcgccgg tattgcccag cactttcatt tccatgctat gcaacgtgcc atccgccatt
     1681 acgccggtct tgtaggtaat cgtctcgggg tggcgcgacc ggctgctgtg aaattcttct
     1741 gaacggttca gctccagtcg cacggggcgg cctgtggcaa tggtcagatg gccgacgata
     1801 tcctcgatca gcatctcctg cttcgcgccg aacccgccac cgatccgcgg cttgatgacc
     1861 cggatacgac tcaccggcag ccccagcaac ggcgcgacca tgcgccggac gtggaacggc
     1921 acttgtgtcg aagtacgaat gaccatgcgc tcatcggcgt cccaccagct gatggcgata
     1981 tgcggctcga tgggcacttg ctgtacctga tgcacgtaat acgtatgctc gaacacgcga
     2041 tcggcctgga caaaaccccc atcgacgtct cccttctcgg cgaagatgtg atggacgatg
     2101 ttgcgcgatg catcgtggat atcttcggtg tcaggctcgt catggatcac cggcgccccg
     2161 ggaagaatgg cttcccgttc atcgaacacg gccggaagca cttcgtactc caccttgatg
     2221 agcttcaggg ctgcttcagc tatttcatcg gtgtccgcag ccacagcggc gacccgatcg
     2281 ccaacgtgac gcaccttgtt gtcgaagctc acctggtcat gaggtggcgg gttcggatac
     2341 gattgcccgc cggatgcgta tttaactcgt tcgacattct tgtaatgcac gacagcgcgc
     2401 acgcccggca gagcacgcgc ctcgctatcg tcgatatcga ggatgcgtgc atgggcatga
     2461 gggctggtca gaagccgggc gtataacatc cctcgcatct cgatatcgtc cacaaatgcg
     2521 gggttaccct tggccagctt gacggcatcc accttcgttt ccggcttgcc cacgacgctg
     2581 gtttccggcg tgtggccttg caagacgatg ctcgttcgcg gccgagtgtc gatggcggtc
     2641 attggacctg gcgcgccgcc gctcagctca gggccggcga aattcgggtc gtcctcctcg
     2701 ccgtcgggcg atctgtattc cgggttttcc accatgtagg tggcatcaac gatgaccgcc
     2761 ccggatagcg gcggcggctc ctcgccgcgc aggattgcgg ccgcccgcaa gatggcttcc
     2821 actggccgca gatagccggt ctcacggcac aagacccccg atagcgcatc gcgaacttca
     2881 gcctcggttg ggttcggatt ccggtcaagc agccccttgg cggcaaggat catcgccggg
     2941 gtcgaatagc cggattggat ggcccccgtc tcgataaacg cttgctgcag agggtgcaac
     3001 tcgccgacgc gggagttcaa actatcgatg gtttcaatac gatggccgac aacctgccca
     3061 acgaccatgg cgtctgtgct gatgaggcgc ccgtcgacta ataccgcggc cgcgcccgtc
     3121 tcgcccgaat ggctgccaaa ccggacactc atatatccgg catcacggag ggtatcgcgc
     3181 aggttgtcct gggtgttggc atgtagctct tgatcttgcc cgttaatatg caaggttaca
     3241 gacattgtca tctcctggag gtctcccggt aggccgagag cttattttgt ttcatccttg
     3301 gtggcagccg tgcgtaatac gttcaggtag ttggctcgtt ccagagcggc cgaatctccg
     3361 atttgcgcat ggctcagcag cccgcgaatt tcgctgactg aatgataggc atttgcatca
     3421 agccagccgg cgagaccatc ggtcaaggtt ttcaggtaat cgatgccgtt ctgaatcaat
     3481 accgaagtca tcatcaccac gttggcgccg gccatcaatc ccttcaccac atcctcgacc
     3541 gtatggacgc caccggtaat cgccaaatcg gctttgatgt gctgcgccag caaggcaacc
     3601 caccgcaagc gcagccgtaa ctcgctggat gtactcagat cgaggctggg aatgatcgcc
     3661 cggttgacga tgtcgaaatc gggttggtag aaccggttga acaagacgag gccatgcgcg
     3721 cccgctccca ccagtcgatg cgcgaaattg gccatcgcgc tgaaataggg gctgagtttc
     3781 accgataccg gtatacgcac cgatgcagtc acatcgcgca ccagatcaat atacatctgc
     3841 tcgacttcgt cggccgtgat atgagctttc gtgggcaggt agtagatgtt gagttcaagc
     3901 gcatcggcgc ccgcggcttc aatgagtcgc gcatatcggg tccagccgct gctgtaataa
     3961 ccgttcaggc tgccgatgat ggggatcgac agggcatgcc tggcctgata aatatgggcg
     4021 agatatctgc cggctccctg gttatattcc ttcatattgg cgacgtgtcg tagcgcctcg
     4081 ggcagttcat cggcatgttg tttgtagtag ctcgtgaagc cca
//
LOCUS       DAOMQG010000317         4117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9009_length_4117_cov_5.919252, whole genome shotgun sequence.
ACCESSION   DAOMQG010000317 DAOMQG010000000
VERSION     DAOMQG010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9009_length_4117_cov_5.919252"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLC06_13710"
     CDS             complement(<1..296)
                     /locus_tag="PLC06_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91548.1"
                     /translation="MKAQLVPIYFDPGRDEGFDVQLARLGELLADDAEFLPPVALGAT
                     LPPQAHAAVFPQMLGEAYRRVDDFRRIDLPILVVTSEFGTLNMWDWEIVAYLEG"
     gene            complement(358..762)
                     /locus_tag="PLC06_13715"
     CDS             complement(358..762)
                     /locus_tag="PLC06_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRO91549.1"
                     /translation="MDGSGRVIRNHNDLDVYRMAFDAAMRIFELSRGFPPEERYALTD
                     QIRRSSRSVAANLAEAWRRRRYQAAFTQGLNRCEAEAAEAQVWLEFAVKCGYLDREQG
                     RILYAEYDHIIGKLVNMINHPAPFLLYGSEKR"
     gene            complement(811..1758)
                     /locus_tag="PLC06_13720"
     CDS             complement(811..1758)
                     /locus_tag="PLC06_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRO91550.1"
                     /translation="MTHYPITTQPQPTMYFIGVTTGKSSINKVFPLWMDVLGRPEVVL
                     RGIDHPIHDDAEAYRRSVAQIKHDPLSLGALVTTHKIDLFNAARDMFDYFDPYARITG
                     ELSSISKRDGRLEGHAKDPITAGLSLEAIIGDGYFGRTGGHILCLGAGGSAVATLLHL
                     INKPDAADRPARFVVVNRTQGRLDHMREMVEQVGTDIEVVYVCNEDAAENDRLMAALP
                     DYSVVINATGMGKDSPGSPITDTGVFPRRGVAWEFNYRGELDFMHQALRQVDGRELTV
                     EDGWIYFVHGWSQVVAQVLHVDLTPEVFAALERAAAGVR"
     gene            complement(1856..2500)
                     /locus_tag="PLC06_13725"
     CDS             complement(1856..2500)
                     /locus_tag="PLC06_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRO91551.1"
                     /translation="MSKQETLNRLHDLGLVAVIRGPSPELTVEMVAALVAGGVTGIEI
                     TYTTPNAAEVVRALDRAFGDRILLGMGTLTDPAQAAEALNAGARYLVSPITDDALAAA
                     MVATGLPLMIGALTPTEVFRAHQLGSDVVKLFPGSAVGPDYVKALHGPFPHIPIMPTG
                     GVDEHNLADWFRAGVWAVGAGSQLSPVKLAREGKFDEITAIAARFAAAVAKART"
     assembly_gap    2536..2635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2697..3665)
                     /locus_tag="PLC06_13730"
     CDS             complement(2697..3665)
                     /locus_tag="PLC06_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRO91552.1"
                     /translation="MKLLNECHILVTPASYGQHDAGLKTELEAAAGRVTYNETGKPLS
                     SAQLAALLPGVDGLIAGLDTIDAAALAAADSLRVVARYGVGTDNVDLAAARARGIVVT
                     NTPGANAGAVAELAVTLLLLLARPVLGAAAATREGGWPRTSGLSLGGKTVGLVGFGAI
                     GRAVARLLSGFGCRVLAYDPHIDPDQAAELGVTVVELEQLVAASDFVSLHAPATPQTR
                     GLVNAEFLARMKPGAALVNTARGELVDEAVLHEALVSGRLRGAALDAFAHEPPGAANP
                     LLSLPNVIATPHMGAHTDDATTTMGRMALADCLAVLRGEEPVHRVM"
     gene            complement(3788..>4117)
                     /locus_tag="PLC06_13735"
     CDS             complement(3788..>4117)
                     /locus_tag="PLC06_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HRO91553.1"
                     /translation="YMTKGHFHTVLETAEIYVCLAGQGVMVMETPEGDWAVEPLAPGR
                     VLYVLPRWAHRSVNTGAADLITFFAYPGHAGHDYGTIEQQGFRKLMVERDGLPEIVDN
                     PRWGRGG"
BASE COUNT          676 a         1366 c         1277 g          698 t
ORIGIN      
        1 tccccttcga gataggcgac gatctcccag tcccacatgt tgagcgtgcc gaactcggac
       61 gtgacgacga ggatgggcag gtcgatgcgg cgaaagtcgt cgacgcggcg gtaggcctcg
      121 cccagcatct gggggaagac ggccgcgtgg gcctgcggcg gcagcgtcgc gcccagcgcc
      181 accggcggca ggaactcggc gtcatcggcc aacagctcgc ccagccgcgc cagttgaacg
      241 tcaaaccctt cgtcccgtcc ggggtcgaag tagatgggaa ctaactgtgc cttcatgata
      301 cctccatttg atttgaaagg gggaaaggga gaaagggaga aggggagcgg ggaggcgtca
      361 tcgcttttcg cttccataga gaagaaaggg ggcggggtgg ttgatcatgt tcaccagctt
      421 gccgatgata tgatcgtatt cggcgtagag aattcgccct tgctcacggt cgagatagcc
      481 gcatttgacg gcaaactcca gccaaacctg cgcttcggcc gcttccgctt cgcatcggtt
      541 gagaccctgg gtaaacgccg cctgatagcg ccggcggcgc caggcctcgg ccaaattggc
      601 ggccaccgag cgagatgacc gccgaatctg atccgtcagc gcgtacctct cctccggcgg
      661 aaacccgcgg ctcaattcaa atatcctcat cgccgcatca aaagccatcc tgtacacgtc
      721 caaatcatta tgattccgaa tcaccctacc cgacccgtcc ataccctttc tccccttccc
      781 cctttctccc cttctccctt tccccccttc tcatcttact ccggccgccg cccgctccag
      841 cgccgcgaac acctccggcg tcagatcgac gtggagcacc tgggccacca cctgcgacca
      901 gccgtggacg aaatagatcc agccgtcctc cacggtcagc tcgcggccgt ccacctgccg
      961 taaggcctga tgcatgaaat ccagctcgcc gcggtagttg aactcccagg ccacgccgcg
     1021 ccgcgggaac acgccggtgt cggtgatggg cgagccgggc gaatccttgc ccatgcccgt
     1081 ggcgttgatg accacgctgt agtcgggcag cgcggccatg agccggtcgt tttcggccgc
     1141 gtcctcattg cagacgtaga ccacctcgat gtcggtaccc acctgctcca ccatctcgcg
     1201 catgtggtcg agccgcccct gggtgcggtt gaccacgacg aagcgcgccg ggcgatcggc
     1261 cgcgtcgggc ttgttgatca ggtgcagcag cgtggccacg gccgacccgc ccgcccccag
     1321 gcagaggatg tggccgccgg tgcggccgaa atagccgtcg ccgatgatcg cctccaggct
     1381 cagcccggcg gtgatagggt ccttggcgtg tccctccagg cggccgtcgc gtttggagat
     1441 cgacgacagc tcgccggtga tccgcgcgta ggggtcgaag tagtcgaaca tgtcccgcgc
     1501 ggcgttgaac aggtcgatct tgtgcgtcgt caccagcgcg ccgaggctga gcgggtcgtg
     1561 cttgatctgg gccacgctgc gccggtaggc ctccgcgtcg tcgtggatgg ggtggtcgat
     1621 gccgcgcagc acgacctccg ggcggccgag cacgtccatc cacagcggga ataccttgtt
     1681 gatcgacgat ttgcccgtgg tcacgccgat gaagtacatc gttggttggg gctgggtggt
     1741 gattgggtag tgggtcatat atcaattacg aattacgaat tacgaattac gaataaagaa
     1801 gagaatccac agattgcaca gatttcacag atttttctgt gtaatttgtg gatctttagg
     1861 ttcttgcctt cgccacggcc gcggcgaagc gggcggcgat ggcggtgatc tcgtcgaact
     1921 tgccttcgcg ggcaagcttg accggcgaaa gttggctgcc cgcgccgacg gcccataccc
     1981 ccgcccggaa ccagtcggcc aggttgtgct cgtccacgcc gccggtgggc atgatcggga
     2041 tgtgggggaa cgggccgtgg agcgccttca cgtagtcggg gccgacggcc gagccgggaa
     2101 acagcttgac cacgtcggag ccgagttggt gggcgcgaaa cacctcggtc ggcgtcagcg
     2161 cgccgatcat cagcggcagc ccggtggcga ccatcgccgc cgccagcgcg tcgtcggtga
     2221 tggggctgac cagatagcgc gcgccggcgt tcagcgcttc ggccgcctgc gccggatcgg
     2281 tcagcgtgcc catgcccagc aggatacggt cgccgaacgc ccggtcgagc gcgcgaacga
     2341 cttcggccgc gttgggcgtg gtgtaagtga tctcaatgcc ggtcacaccc ccggccacca
     2401 gcgccgccac catctccacc gtcagctccg gcgacggccc gcgaatgacg gcgacgaggc
     2461 cgagatcatg caatcggttc agcgtctctt gcttgctcat aagtccttcc tgtcaagctt
     2521 tcgtgatcgt attcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcttt
     2641 cgtgatcgta ttcgcaatcg gtcttcgatt acgattgcga tagcgattgc gagccgttac
     2701 atcacccgat gcaccggctc ttcgccgcgc aacaccgcca gacaatcggc cagcgccatg
     2761 cgccccatcg tcgtcgtcgc gtcgtcggtg tgggcgccca tgtggggtgt ggcgatgacg
     2821 ttcggcagcg acagcagcgg attggccgcg cccggcggct cgtgggcgaa cgcatccagc
     2881 gccgcgccgc gcaagcggcc ggagaccagc gcctcatgca gcaccgcctc gtccaccagt
     2941 tccccccgcg ccgtgttgac cagcgccgca ccgggcttca tccgcgccag aaattcagcg
     3001 ttcaccaggc cgcgcgtctg cggcgtcgcc ggcgcatgga gcgacacgaa gtcgctcgcc
     3061 gccaccagct gctccagttc gacgaccgtc acgcccagtt cggccgcctg gtcgggatcg
     3121 atgtgggggt cataggccag cacgcggcaa ccgaagccgg aaagcagccg ggccacggcg
     3181 cggccgatgg ccccgaagcc gaccagcccc accgtcttgc cgcccaagga caagcccgac
     3241 gtgcgcggcc agccgccctc gcgcgtggcc gccgccgcgc ccagcaccgg ccgggccagc
     3301 agcagcagca aagtcaccgc cagctcagcc actgccccgg cgttggcccc cggcgtgttg
     3361 gtgacgacga tcccccgcgc gcgggccgcg gccagatcga cattgtcggt gcccacgccg
     3421 tagcgggcga cgacgcgcag cgaatcggcc gcggccagcg ccgccgcgtc gatggtatcc
     3481 agcccggcga tgaggccgtc gacccccggc agcagcgccg ccagttgcgc cgacgacagc
     3541 ggcttgccgg tttcgttata cgtcacgcgg ccggcggccg cttccagttc cgttttcagc
     3601 cctgcatcat gctgcccata ggacgccggc gtgacgagaa tgtggcattc gtttaatagt
     3661 ttcataagaa tgcaggggag caggggtgca ggggagaagg ggggaagggg tgaaagggag
     3721 caggggagtc aagcgcaccc tttctcccct tctccctttc tccctttccc cctatctccc
     3781 tttctcccta tcctccccgc ccccaccgcg gattatcaac gatctccggc aggccgtcgc
     3841 gctcgaccat cagcttgcgg aagccctgct gctcgatggt gccgtagtcg tggccggcgt
     3901 ggccggggta ggcgaagaag gtgatgaggt cggccgcgcc ggtgttgacc gagcgatgcg
     3961 cccagcgcgg cagcacatag agcacgcgcc ccggcgccag cggctccacc gcccaatcgc
     4021 cctccggcgt ctccatgacc atcacccctt gcccggccag acagacgtag atttcggccg
     4081 tctccagcac ggtgtggaag tggcccttgg tcatgta
//
LOCUS       DAOMQG010000318         4104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9039_length_4104_cov_6.194122, whole genome shotgun sequence.
ACCESSION   DAOMQG010000318 DAOMQG010000000
VERSION     DAOMQG010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9039_length_4104_cov_6.194122"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLC06_13740"
     CDS             complement(<1..425)
                     /locus_tag="PLC06_13740"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRO91554.1"
                     /translation="MKSQFRLLLKLFVLLGAVVWSAACGGGEAAGGVPRIAPASLSLE
                     LGDMPNGEIAERELVVRNDGDAPLVVDTITTSCGCTTAVLEPMTIAPGAAGTLRITFD
                     SGAHGPDLRGPLLREVMIASNDPATPEVVVSVTANILEPV"
     gene            complement(442..930)
                     /locus_tag="PLC06_13745"
     CDS             complement(442..930)
                     /locus_tag="PLC06_13745"
                     /inference="COORDINATES: protein motif:HMM:NF025170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HRO91555.1"
                     /translation="MARILLVFVLLSTLALSACAIEPEADEEDAMAHASRLATGPAGH
                     DHPMAPLKDMPEMVRAADARTQEAYRFAAANPDAAAEVPCYCGCVGLGHETSYDCYVA
                     GTTADGALEYDMHAVNCTVCVDITQDQMRMMDDGDSPDTIRAWLAENYAKYGPPTEEI
                     TN"
     gene            complement(1010..1567)
                     /locus_tag="PLC06_13750"
     CDS             complement(1010..1567)
                     /locus_tag="PLC06_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRO91556.1"
                     /translation="MTQARHAKKQARAGRTELIFALAFMGGAVLLLVAGALLANRPVE
                     LTSEGDIVRGRMIRATHQMGEGAPIPFLPKDGPQPNVTVDEAFHDFGRIGPEDVVTRT
                     FVVRNNGDAPLTISRAYTTCGCTTAEVSADEIPPGKAVTVEMVFDAGFHDSAGQTVRR
                     GVILENNDPDQPQTELWVQAEVGRE"
     gene            1741..2406
                     /locus_tag="PLC06_13755"
     CDS             1741..2406
                     /locus_tag="PLC06_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91557.1"
                     /translation="MTARPFIATFPVYMSAPITILLADDHAVVRAGIRRFLETADDLR
                     VVAEAGDGEDARRLIAAHRPDVAVLDIQMPRATGIEVARWTRQEFPATGVLILTAYDD
                     DPYIQAGLAAGANGYVLKTAVPAAMIQAVRDVYEGKQVLDPAIRARAVALDTLFEPLS
                     DRELEVLALAARGYTNKAISAQLFISDRTVQGHLAHIYAKLQTSTRTEAVMRAVSLGL
                     IAP"
     gene            2403..4103
                     /locus_tag="PLC06_13760"
     CDS             2403..4103
                     /locus_tag="PLC06_13760"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRO91558.1"
                     /translation="MTRLRGLPAQLFLFTILPLTLLLAAIAFGGLTLHQRAMRQMVGE
                     RDERATRAAAGALEEQLRRRTAAVSNLALHAAHVPPATALADSVSFRDDFIGLAIFGP
                     DGSLLAADDPAFWEGLEAEFLANGSPATGIMPLITDPATGAPVALATATTRDGGMAAG
                     AFDPAAIARGVLARVVRPGDHSVAYLVAPDGTLLYQAGSGNHAEPITDHPGVAEALRG
                     EIGTTFLGANGGIGGDDHVVSYTPVAPVGWALVMEEVWREMTDPMLRRTELAPFILLP
                     ALAIALAGLWFGARQIVRPLQTLAERTTALGRGDFDAVGEPVGGIGEIRQLQGELIGM
                     ARRLQMAQQGLRDYLGALTAGQEEERHRLARELHDDTIQSLIALNQRIQLAQTRATGE
                     TAAQLAEMGVMATDTIADLRRLTRDLRPSYLDDLGLVPALELLARDQSAALDLPVTFE
                     TTGEPRRPDHGAELAFYRIAQEALRNVGRHARANRAVIALNFTPREIMLTIRDDGIGF
                     DTAERAAERALGGHFGLLGARERAESVSARLEVESAPGKGTTLKVTSAIPQPLQIPNS
                     "
BASE COUNT          699 a         1470 c         1239 g          696 t
ORIGIN      
        1 accggctcca gaatattggc cgtcaccgac acgacgactt ccggtgtcgc cggatcgttc
       61 gacgcgatca tcacctcgcg caacaatggc ccgcgcaggt cggggccgtg ggcgccggaa
      121 tcgaaggtga tccgcagcgt gccggcggcc cccggtgcga tggtcatcgg ctccagcacg
      181 gccgtcgtgc aaccgcagga agtcgttatc gtgtcgacca ccagcggcgc gtcgccgtca
      241 ttgcgcacca ccagttcccg ctcggcgatt tcaccgttgg gcatgtcgcc cagttcgagg
      301 gacaacgacg ccggcgcgat ccgcggcacg ccacccgcag cctcgccacc cccgcaggcc
      361 gccgaccaca ccaccgcacc aagcagtaca aataacttga gtaatagtct gaattggctc
      421 ttcattcctg gttcgtaatt cctaattcgt aatttcttcc gtcggcggcc cgtacttggc
      481 gtaattctca gccagccagg ctcgaattgt gtccggtgaa tcgccatcat ccatcatgcg
      541 catctggtcc tgagtgatat cgacgcacac ggtgcagttc acggcatgca tatcgtattc
      601 cagcgcgccg tcggccgtcg tcccggccac gtagcaatcg taactggtct catgacccag
      661 ccccacgcag ccgcaatagc acggcacttc ggccgccgcg tcgggattcg ccgccgcaaa
      721 gcgataggcc tcctgcgtcc gcgcgtcggc agcgcgcacc atctccggca tgtccttaag
      781 cggggccatc ggatggtcgt ggcccgccgg cccggtcgca aggcgcgaag cgtgggccat
      841 cgcgtcctct tcatccgcct ccggctcaat cgcgcaggcc gagagcgcca acgtagacaa
      901 caggacgaag accaacagaa ttcgagccat accgaccccc acccaccgtt ccatcaaaaa
      961 tcacccaccc atgacgaatc aaccgtccgg gttcattcgt aattcgtaat tactctcttc
     1021 ccacttccgc ctgcacccac agctccgtct ggggctggtc ggggtcgtta ttctccagaa
     1081 tgaccccacg gcgcaccgtc tgcccggccg agtcgtgaaa gccggcgtcg aagaccattt
     1141 caacggtgac cgccttgccc ggcgggatct catcggcaga gacctcggcc gtggtgcagc
     1201 cacaggtcgt ataggcgcgg ctgatcgtca gcggcgcgtc gccattgttg cgcaccacaa
     1261 acgtgcgcgt caccacgtct tccggtccga tgcggccgaa gtcatggaac gcctcgtcca
     1321 cggtcacatt cggttgcggg ccatccttcg gcaggaacgg gatcggcgcg ccctcaccca
     1381 tctgatgcgt ggcccggatc atcctcccgc gaacgatgtc cccctcgctt gtgagttcaa
     1441 ccggccggtt ggccagcagc gcacccgcca ccaggagcaa caccgcccca cccataaacg
     1501 ccagcgcgaa tatcaattcc gtgcgcccgg ctctagcctg tttctttgcg tgtcttgctt
     1561 gtgtcatgat tcacccttga ataaaggagg cggccgaccc gtttcgactg ccgccggcca
     1621 cccccatcct gcgagttctg cccatatcat acgttctcgc cctacccttc cacagcgcca
     1681 aatggcgtat ttcgcgacaa gccctcgata gcgacgcgca catcccgaaa cccacatccc
     1741 ttgaccgccc ggccatttat cgctaccttt ccggtctata tgagcgcgcc gataaccatc
     1801 cttctggccg atgatcatgc cgtggtccgg gccggcattc gccggtttct ggagaccgcc
     1861 gacgatctgc gcgttgtggc cgaagccggc gacggcgagg acgcccgccg cctgatcgcc
     1921 gcccaccggc cggatgtggc cgtgctggac atccagatgc ccagagccac aggcattgaa
     1981 gtcgcccgct ggacgcgcca ggaattcccg gcgaccggcg tgctgatcct gacggcctat
     2041 gacgacgatc cgtatatcca ggccgggctg gccgccggag ccaacggcta tgtgctgaag
     2101 acggccgtgc cggccgccat gatccaggcc gtgcgggacg tatacgaggg caagcaggtg
     2161 ctggacccgg ccatccgcgc tcgcgcggtc gcgctcgaca ccctgttcga gcctctgtcc
     2221 gatcgcgagc tggaagtcct ggccctggcc gcgcgtggct acacgaacaa agccatcagc
     2281 gcgcagctct ttatcagcga ccgcaccgtg caggggcatc tggcccacat ctatgccaag
     2341 ctgcaaacca gcacgcgcac ggaggccgtc atgcgcgccg tctcccttgg cctgatcgcc
     2401 ccatgacccg cttgcgcggc ttgcccgccc aactttttct cttcacgatc ctgccgctga
     2461 cgctgctcct ggcggccatc gccttcggcg gcctgacgct ccaccagcgc gccatgcgcc
     2521 aaatggtggg cgagcgggat gagcgggcca cccgggccgc cgccggcgcg ctggaggaac
     2581 aactgcgtcg ccgcaccgcg gccgtgagca atctggccct gcatgccgct cacgtgcccc
     2641 ccgccaccgc cctggccgac agcgtttcgt tccgggatga cttcatcggc ttggccatct
     2701 tcggccccga cggctcgcta ttggcggccg atgatcccgc tttttgggaa gggctggaag
     2761 cggagtttct cgcaaacggg tcgccggcta ccggcatcat gccgctgatt accgacccgg
     2821 caaccggcgc ccccgtcgcc ctggccacgg ccacgacgcg cgacgggggc atggcggccg
     2881 gcgcgttcga cccggccgca atcgcccgcg gcgtgttggc ccgcgtcgtc cggcccggcg
     2941 atcattccgt cgcctatctg gtcgcgccgg acgggacgct tctttaccag gccggctccg
     3001 ggaaccacgc cgagccgata accgatcacc ccggcgtagc cgaggcgctg cgcggagaaa
     3061 tcggcacaac ctttctgggg gccaacggcg gcatcggcgg cgacgaccac gtggtcagct
     3121 acacccccgt cgcgccggtc ggctgggctt tggtgatgga agaggtctgg cgggaaatga
     3181 ccgacccgat gctgcggcga acggaactgg cgccctttat cttgctcccg gcgctggcca
     3241 tcgccctcgc cggactgtgg ttcggcgcgc ggcaaatcgt tcgcccgctg caaacgctgg
     3301 ccgagcgaac caccgccctc ggccgcggcg atttcgatgc cgtgggcgag ccggtgggcg
     3361 gcatcggcga gattcgccaa ttgcaagggg agttgatcgg gatggcccgc cggctgcaaa
     3421 tggcccagca gggactgcgc gattacctgg gggctttgac ggccgggcag gaagaagaac
     3481 gccaccggct ggcccgcgag ttgcacgacg acaccatcca atccctgatc gccctcaacc
     3541 agcgcattca gttggcccag acgcgggcaa ccggcgagac ggccgcgcag ttggccgaga
     3601 tgggggtgat ggcgaccgat accatcgccg atttacgccg cctgacgcgc gatctgcggc
     3661 cgagctatct ggacgacctg gggcttgtcc cggcgctgga gttgctggcc cgtgaccagt
     3721 cggccgcgct cgacctgccg gtgacgtttg aaacaaccgg cgaaccccgc cgccccgacc
     3781 acggggccga actggccttc tatcgcatcg cgcaggaagc gttgcgcaat gtcggccgcc
     3841 atgcccgcgc caatcgggcg gtcatcgccc tcaatttcac ccccagggaa atcatgctga
     3901 cgattaggga cgacggcatc ggcttcgaca cggccgaacg ggccgcggag cgcgcactgg
     3961 gcggccactt cggcctgctc ggcgcgcgcg aacgagccga gtcggtcagc gcgcggctgg
     4021 aggtcgaatc cgcgcccggc aaggggacga cgctgaaagt gacaagcgca atcccccaac
     4081 cgcttcaaat tcctaattcc taat
//
LOCUS       DAOMQG010000319         4098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9052_length_4098_cov_9.770467, whole genome shotgun sequence.
ACCESSION   DAOMQG010000319 DAOMQG010000000
VERSION     DAOMQG010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9052_length_4098_cov_9.770467"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLC06_13765"
     CDS             <1..581
                     /locus_tag="PLC06_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91559.1"
                     /translation="LNGPKLEQTFSFAQAVEVAPEAAAGSDAPATYTEFVGIYDDSQA
                     VYVEVPVEWADVDGSNWLADDGSVLGSSIMASTDIAGFSSTYTTPGMQILAGTVFGNS
                     TMGELADILDFSDSCTYDGRFDYDDGFYTGVYDQYSNCDGQGSVIIVLAAEPAEQNYS
                     VIVLVQAVTDADLDALDHILDTFIVVGELPGG"
     gene            complement(669..3494)
                     /locus_tag="PLC06_13770"
     CDS             complement(669..3494)
                     /locus_tag="PLC06_13770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRO91560.1"
                     /translation="MPTTYVAVDVETTGLDPSRDEIIEVAAITFRDAAIVDEFNSLVN
                     PQIDIPPFITQLTTITNEMVADAPTLHTLRPRLRSKLGDNVLVGHNIEFDLGFLREAR
                     LAFGNRRIDTLTLASILVPEAGRFSLSALADFLALPADNGRGHRALADAELTIELFLA
                     LRERALALSLAQLEEIVSAGQRLGWPETLFFEEVLAERVRDAFEGNELRMRGQLQRLY
                     TPPKLEGATLVPKEHPAPLDVASLTSLIRPGGRFDRAFPGFEHRPQQVEMLQTVAEAF
                     NSGRHVLVEAGTGTGKSLGYLIPAAYWAIDNERRVVISTNTINLQDQLIDKDIPALQK
                     ALHLDLRSAIRKGRSNYICTRLFQQMRRTGPGNADEMALYARILLWLPHSQTGDVGEI
                     ALRTPGERQAWSKINSENPTCTPDQCAAENCPLHIARRRSEMAHLVVVNHSLLLADLR
                     SEGKVLPDFQDLIIDEAHHLEAAVTDGLSFRADRRSLEAILEEVTRPRAGLVANAQQR
                     IAAVAPRETGTEVDAIAERMRGDAQLATLRTEEFFTTLDFFLRGQQRERSQFATQIRI
                     TPAVRTQPDYHLIEESWADLTKLYTVLVKGFQKLDQIVAELSHVVDIEGSDELRAALV
                     SNGRLLEEAKVNLDALVLSPDSAMIYWVEQFKDFISLHAAPLHVGPLVEAHIFQSKET
                     VVLTSATLRTAGHGRAESTFDYVRDRLHAHEADELAVGSPFDYKNSTLLYLVTDIPEP
                     NQPGYQKMLEQAIIDTATTLGGRTLVLFTANNHLSQTATAIESKLAAQKITTLAQNQG
                     ASRQQLLEQFRREGNRAVLLGTRSFWEGVDIPGSALTALIIAKLPFDVPTDPIFAARS
                     ETYESPFFEYSIPEAVLRFRQGFGRLIRRGSDEGVVLVLDKRVITKRYGQAFIEALPD
                     CTVIRQRSDRIEELLARWMARDRNKRL"
     gene            complement(3621..>4098)
                     /locus_tag="PLC06_13775"
     CDS             complement(3621..>4098)
                     /locus_tag="PLC06_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91561.1"
                     /translation="IPPTPVPSSSPTPTISSPVAMQPALRNISGGVTSCPDHPYINSE
                     KPVTDPRFLLRVDAQLFDPPPGESGNGAGIDHVEFLVNETGFTHTERVAGYCIFQGGE
                     PDCRDWPRDALGRLTWGEDGPLVVDGDYRITVTIYPKPETAATQCHWNVLLNIDVP"
BASE COUNT          783 a         1278 c         1273 g          764 t
ORIGIN      
        1 agctcaatgg ccccaaactg gaacagacct tctccttcgc tcaggccgta gaggtagccc
       61 cggaggcggc ggccggcagc gatgctcccg ccacctacac ggagttcgtc ggcatctacg
      121 acgactctca ggcggtatac gtcgaagtgc cggtggagtg ggccgacgtc gacggctcca
      181 actggctcgc cgacgacggt tccgtcctcg gcagctcgat catggcctcc accgacattg
      241 cgggcttctc ttctacctat acgacccccg gcatgcagat tctggcgggc actgttttcg
      301 gcaactccac catgggcgaa ctggcggata tcctcgactt ttccgactcc tgcacctatg
      361 acggccgctt tgattatgat gacgggttct ataccggcgt ctacgatcaa tactccaact
      421 gtgacggcca gggcagcgtt atcatcgttc tggcggccga accggcggag cagaactact
      481 ccgtgatcgt gctagtgcag gccgtcaccg acgcagacct ggacgcgctg gaccacattc
      541 tcgacacgtt tatcgtcgtc ggcgagctgc cgggaggctg aggctaacag ccggccaatt
      601 gacataagaa aaaggtaggt catcgacctg ccttttattt tcgacgcgag ccgatttgaa
      661 atcggccttt acaggcgctt gttgcgatcg cgggccatcc agcgcgcgag cagctcctcg
      721 atgcgatccg accgctggcg gatgacggtg caatcgggca gcgcttctat gaacgcctgc
      781 ccgtagcgct tggtgatcac ccgcttgtcg agcaccagta ccaccccctc atcactgccg
      841 cggcgaatca gacggccaaa cccctgccgg aagcgcagca ccgcctcagg gatcgaatac
      901 tcaaagaacg ggctttcgta ggtctccgag cgggccgcga atattgggtc ggtcggcaca
      961 tcaaacggca gcttggcgat gatcagcgcc gtcaacgcag agccggggat atcaacgccc
     1021 tcccagaatg agcgcgtgcc cagcagcacc gcccgattgc cctcgcgccg gaactgctcc
     1081 agcaattgct gccgcgacgc gccctggttc tgggccagcg tggtgatctt ttgcgccgcc
     1141 agcttgctct cgatagccgt cgccgtctgg ctcaaatgat tgttggccgt gaaaagcacc
     1201 aaggtgcggc cgcccagcgt ggtggccgtg tcgatgatcg cttgctccag catcttttgg
     1261 tagcccggct ggttgggttc ggggatgtcg gtcaccagat acagcagcgt cgaattcttg
     1321 taatcgaacg gagagccgac ggccagttcg tccgcctcgt gggcatggag ccgatcgcgg
     1381 acgtaatcga atgtcgattc cgcccgacca tgaccggcgg tgcgcagcgt ggccgaggtc
     1441 agcaccaccg tctccttgct ctggaagatg tgagcctcca ccagcgggcc gacatgcagc
     1501 ggcgcggcat ggagcgagat gaaatccttg aactgctcga cccagtagat catcgccgaa
     1561 tcgggactca gcacaagcgc atccagattg accttggctt cctccagcaa tcgcccattg
     1621 ctcacgagcg ccgcgcgcaa ttcatcgctt ccctcaatat ccaccacatg ggacaactcg
     1681 gccacgatct gatccagctt ctggaaccct ttcaccagca cggtatagag tttggtcaga
     1741 tcggcccagc tctcctcaat caaatggtag tcgggctggg tgcgcacagc cggcgtgatg
     1801 cggatttggg tcgcaaactg gctgcgctcg cgttgctggc cgcgcaggaa aaagtccagc
     1861 gtggtgaaga actcctccgt gcgcagcgtg gccagttggg cgtcgccgcg catgcgctcg
     1921 gcgatagcgt ctacctctgt gccggtttcg cgcggagcca cggccgcgat gcgctgctgg
     1981 gcgttggcga ccaggccggc gcgcggccgc gtcacttcct ccaggattgc ctccagagaa
     2041 cggcggtcgg cgcgaaagct gaggccatcg gtgacggccg cctccagatg atgagcctca
     2101 tcgatgatca agtcctggaa gtcgggcagc accttgcctt ccgagcgcaa atcggccagc
     2161 agcagcgaat ggttgacgac gaccagatgg gccatctcgg agcgacggcg agcgatatgc
     2221 agcggacagt tctcggccgc gcactgatcg ggggtgcagg tcggattctc gctgttgatc
     2281 ttcgaccacg cctggcgctc gccgggggtg cgcagggcta tctcgcccac gtcgcccgtc
     2341 tgcgaatggg gcagccaaag caggatgcgg gcgtagagag ccatttcgtc ggcgttgccg
     2401 gggccggtgc ggcgcatctg ctggaacagg cgcgtgcaaa tgtagttgct gcgccccttg
     2461 cggatggccg agcgcaagtc gagatgaagc gccttttgca gtgcgggaat atctttatca
     2521 atcagctggt cctgcaaatt gatcgtgttg gtggaaatga cgacccggcg ctcattatct
     2581 atcgcccagt atgcggccgg aatgagatag cccaggctct tgccggtgcc tgtgccggcc
     2641 tccaccaata cgtgtcggcc gctgttgaac gcctcagcga cggtttgcag catctccacc
     2701 tgttgcggac ggtgctcaaa gccggggaag gcgcgatcga agcggccgcc ggggcgaatg
     2761 agcgaggtca gcgaagccac atcgagcggc gcgggatgct ccttcggcac cagcgtggcc
     2821 ccttccagct tcggcggcgt atagaggcgc tgtagctgcc cgcgcatgcg caactcatta
     2881 ccctcgaacg cgtcgcggac gcgctcggcc agcacctcct caaagaagag cgtctccggc
     2941 cagccaagcc gctgcccggc ggagacgatc tcctccagtt gggccagcga cagggccagc
     3001 gcccgttcac gcagcgcgag gaacaactca atagtcaact cggcgtcggc caacgcgcga
     3061 tgaccacgac cattgtcggc cggcagagcc agaaagtcag ccagcgcgct caggctgaac
     3121 cgccccgctt ccggcacgag aatcgaagcc agcgtcaatg tgtcgatgcg ccggttgccg
     3181 aaagccagcc gcgcctcgcg cagaaagccc aggtcgaact cgatgttgtg acccaccagc
     3241 acgttatcgc ccagcttgct gcgtagtcgc ggccgcaagg tgtggagcgt cggcgcgtcg
     3301 gccaccatct cgttggtgat cgtcgtcagt tgggtgatga acggcgggat atcgatctgt
     3361 gggttgacga gcgagttgaa ctcgtcaacg atggcggcgt cacggaaggt gatcgccgct
     3421 acctcgatga tctcgtcacg cgaagggtcc agcccggttg tctccacatc gaccgcgaca
     3481 taggtggttg gcatggagag atcgtatcct atatgcggca acaattccag agaaaatagc
     3541 tcaaatggtc gaatcaattt ggcgatcgat gtagccaaat gagcagaatg caggttctcg
     3601 ccatagcctc gccccaatcg ctagggcaca tcgatgttca acaggacgtt ccagtggcac
     3661 tgggttgcgg ccgtctccgg tttcgggtag atggtgaccg tgatgcgata atcgccgtct
     3721 acaaccagcg gcccatcttc cccccacgtg agccggccaa gcgcgtcgcg tggccaatcg
     3781 cgacaatccg gctcgccccc ctgaaagata cagtaaccgg ccacccgctc cgtgtgggta
     3841 aagcccgtct cgttcaccag aaactctaca tggtcgatgc ccgcgccgtt gcccgattcc
     3901 cccggaggcg gatcaaatag ctgagcgtca acgcgcaaca ggaagcgcgg gtcggtcacc
     3961 ggcttttcgg aattgatata gggatgatcc gggcaggacg tgacgccgcc actgatgttg
     4021 cgcagcgcgg gctgcatggc gaccggcgat gaaattgtcg gagtcggtga cgaggacggg
     4081 acaggcgtcg gcgggatg
//
LOCUS       DAOMQG010000320         4089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9080_length_4089_cov_8.433069, whole genome shotgun sequence.
ACCESSION   DAOMQG010000320 DAOMQG010000000
VERSION     DAOMQG010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9080_length_4089_cov_8.433069"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4079)
                     /locus_tag="PLC06_13780"
     CDS             complement(<1..4079)
                     /locus_tag="PLC06_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRO91562.1"
                     /translation="MMQDKLRRSGITLLLLFLLVFLFSLPLSAAPQSDPSSGDGVPTR
                     LTQANRPRQPITTSSSAADEAIQKLSPDLKQLVDRPGDAESQMVFILVQPGTSVSHLL
                     QRSATSRPVGDVQWVTGEVAINNLVRLASVDGVIAVTSTNTYQPLPAPGLEGMRGDTF
                     NPLITSEAAAALLARGGKAAVMAQVKADNPPPAVESARPDTIRPQAIDSVKAADIHSV
                     NEAHAAGYTGQGVVAAVVDTGVDFAHPDLQGRQARVVSGPYEGWPYAYDTLSGANYAF
                     SSFSRGPDNYWVGVADSQYVHTFPVENAECDGTTCVGELMIDYGDLVPGNNWPSVALD
                     FTWPDTSQSGNYYYTVYPIFDHLFRGFLNGLGYASMYDAPAAIIVADETTSGQYDTVY
                     IDGDFDQDLVEEKAVRKGDELSGADVNDSAYGPAPDGFWDLSTSMLTWIADGTNPPPG
                     VAVLYSGVETPQAGRLLAFVSDAETHGTNVASMIAAQSVITDPDLMSSVNPLFAGGAN
                     VGGVGGPVLAGMAPDTHIAAFMNGFVLPYDAWTLAALGFDGIPESGDEAQIVNNSWGN
                     SVEAADGWDVTSRYAQFLNRNFAPTTTFLAATGNGGPGYGTTTSPNGGSIYKIGASTS
                     YGTSDYFELVGPEQFTWGVVQPWSNRGPGSLGDVDPDLVCVGAYGLGATPLNRFSNGQ
                     ASYDLFGGTSMASPVCAGITALGYQAFQSTHNRWPTWQEAADFLSSGADDLGYNVLAQ
                     GAGNANALRSATIAAGDAAWVTPSQWLPGDYRGEDFTPGFPAIVHAGDTVSTPLTIHN
                     PTDAPVTFAMSDVNLQRVHEITFTVTLGAGNKDTDPIYRIPDYLQDITGLIDEYDPDL
                     IRAHTLIPYNVFDRNGDYSPDNTVTSLFYDWTDLNENGSLWVDGNGNGLVEPKEIDVE
                     RGVPGAYEINRYTYAYSTSSYDVVDIGRDALSRQHDGVYFGLQRGYGSEDIDVTVSLI
                     LYKKADWEWLSLSANSVTVPAGGETDVTATMAVPADAVTGLYEGAIEYNGQVVPVVAH
                     VAADSATFQFGAASTDEPLGDTPHDNGHIFGSTDWSWRPETGDWRHFFYDLPDGTAGP
                     GTVMMVETEWVNPLPDSSIPFPPTALHEEFESGIPWDWVVVDNAGSCAWNVNDPFSIP
                     NYTGGEGLTAAADSASCGPDTVMDTEMHTPPLDFSAADEVWLTFRADFYPYTDGEGNP
                     IDHAYLDISTDGGSNWTTLLDIDYPIWGPSLILQDLSAYAGEPQVTVRFRYVAPGWHA
                     WWQVDDVNVYLGDPGPSLDTLLPDLTDVDTAIFGANEESFSTGDPAFFGPTGLTKLGA
                     SENTLLYGGTWSFQTATGGPREVVGAPMRDGLGLVTLHNV"
BASE COUNT          726 a         1188 c         1418 g          757 t
ORIGIN      
        1 acattgtgga gggtgacgag gccgagaccg tcgcgcatcg gcgcgccgac tacttcgcgc
       61 gggccgccag tggctgtctg gaaactccat gtgccgccat agagcagggt gttctcgctg
      121 gctcccaact tcgtcaggcc ggtggggccg aagaaggccg ggtcaccggt ggagaaggat
      181 tcttcgtttg cgccgaagat ggccgtgtcc acgtcggtga ggtcggggag gagcgtgtcg
      241 agagacggtc cggggtcgcc cagatagacg ttgacatcat ccacctgcca ccaggcatgc
      301 caaccgggtg ctacatagcg gaatcgcacg gtcacctggg gttccccggc atacgcgctc
      361 aggtcctgga ggatgagtga cggcccccaa atcgggtaat cgatgtcgag cagggttgtc
      421 cagttggagc ctccatcggt gctgatatcg aggtaggcat ggtcgatggg attcccctct
      481 ccatcggtat aggggtagaa gtcggcgcgg aaggtgagcc agacctcatc ggcggccgag
      541 aagtcgagcg gcggcgtgtg catctccgta tccatgacgg tgtcgggacc gcagctggcg
      601 ctgtcggcgg ccgccgtcag tccttcgccg ccggtgtagt tggggatgga gaacgggtca
      661 ttgacattcc aggcacacga accggcattg tcgacgacca cccaatccca cgggataccg
      721 ctctcaaact cctcgtggag tgcggtcggc gggaagggga tgctgctgtc ggggagggga
      781 ttgacccatt ccgtctcgac catcatcacc gtgcccggcc cggccgtgcc gtcgggcagg
      841 tcatagaaga agtggcgcca atcacccgtc tccggccgcc aggaccagtc ggtggaaccg
      901 aagatgtggc cgttgtcgtg gggggtgtcg ccgagcggct cgtcggttga ggccgcgccg
      961 aactggaagg tggcggagtc ggcggccacg tgagcgacga ccggcacgac ctgtccgtta
     1021 tactcgatag ctccctcgta gagaccggtt acggcgtcgg cgggcacagc catcgtcgcc
     1081 gtaacatctg tctcgccgcc cgcggggact gtgaccgaat tggctgacag gctcagccat
     1141 tcccaatcgg cctttttgta gaggatgagg ctgacggtga cgtcgatatc ctcgctgccg
     1201 tagccacgct ggaggccaaa gtacacgccg tcgtgttgcc tcgacagcgc gtcgcggccg
     1261 atatcgacca cgtcgtagct gctggttgag taggcgtagg tgtagcgatt gatctcgtaa
     1321 gctccgggca cgccgcgctc gacgtcgatc tctttcggct cgaccaggcc gttcccgttg
     1381 ccgtcgaccc acagactgcc gttttcattc agatcggtcc agtcgtagaa gagcgaggta
     1441 acggtgttgt cggggctgta gtcgccgttc cgatcgaaga cattgtaggg gatgagcgtg
     1501 tgcgcccgaa tgagatcggg atcgtattca tcaatcaacc cggtgatgtc ctgcaggtaa
     1561 tcgggtatcc ggtagattgg atcggtgtcc ttgttgcccg cgcccagcgt gacggtgaaa
     1621 gtgatctcgt ggacgcgctg cagattgacg tcgctcatgg cgaacgtcac cggcgcgtcg
     1681 gtcgggttgt ggatggtcag cggagtcgag acggtatcgc cggcgtggac gatggccggg
     1741 aagccgggcg tgaaatcctc gccgcgatag tcgccgggaa gccattggga cggcgtgacc
     1801 caggcggcat ctccggcggc aatagtcgca gaccgcagcg cgttggcgtt gcccgcgccc
     1861 tgggccagaa cgttgtagcc caggtcatcg gcgccgctgc tcaggaaatc ggccgcttcc
     1921 tgccaggtgg gccaacggtt gtgggtgctt tggaacgcct ggtagccgag ggcagtgata
     1981 cccgcgcaga ccgggctggc catcgacgtg ccgccgaaca ggtcatagga cgcctgcccg
     2041 ttggagaagc gattgagcgg cgtggctccc agaccgtagg cgccgacgca aaccaggtca
     2101 ggatcgacgt cacccaacga acccgggccg cggttggacc acggctgcac gacgccccag
     2161 gtgaactgct ccggccccac cagctcgaag tagtcgctgg tgccgtagga agtgctcgcg
     2221 ccgatcttgt agatcgagcc gccattgggc gatgtggttg tgccgtagcc ggggccgcca
     2281 ttgcccgtgg cggccaggaa ggtcgtcgtc ggggcgaagt tgcgattcag gaactgggcg
     2341 tagcgggagg tgacgtccca gccgtcggcc gcttcgacgc tattgcccca ggagttgttg
     2401 acgatttgcg cttcgtcgcc gctctcaggg ataccgtcga accccagcgc tgccagcgtc
     2461 caggcgtcat agggcaggac gaagccgttc atgaaggcgg cgatgtgcgt atcgggcgcc
     2521 attccggcca gcacggggcc gcccacgccg ccgacatttg cgccaccggc gaagagcgga
     2581 ttgaccgagc tcatcaggtc aggatcggtg atcacgctct gggcggcgat cattgaggcg
     2641 acgttggtgc cgtgagtctc ggcgtcgctg acgaaggcca acaagcggcc ggcctgtggc
     2701 gtttcgacgc cgctatagag tacggcgacg cctggcggag ggttcgtgcc gtcagcgatc
     2761 caggtcaaca tgctcgtcga caggtcccag aagccgtcgg gcgccggccc ataagccgaa
     2821 tcattgacat cagctcccga cagttcgtca cctttgcgca ccgccttctc ttcgaccagg
     2881 tcctgatcga agtcgccatc gatgtagacg gtgtcgtatt ggccggatgt cgtctcgtcg
     2941 gccacgatga tcgccgccgg ggcgtcgtac atcgaggcat agcccaggcc gttgaggaag
     3001 ccccggaaga gatgatcgaa gatggggtag acggtgtagt agtagttgcc gctctgcgaa
     3061 gtgtcgggcc aggtaaaatc gagggcgacg gacggccagt tattgccggg caccaggtcg
     3121 ccgtagtcga tcatcaactc accgacgcag gtcgtgccgt cacattccgc gttttctacc
     3181 gggaaggtat ggacgtattg ggaatcggcg acgccgaccc aatagttatc cggcccgcgg
     3241 ctgaagctgc tgaaggcgta gttggccccc gacagagtat cgtaggcata gggccagccc
     3301 tcatacggtc cgctgaccac tcgagcctgg cggccctgca aatcgggatg ggcgaagtct
     3361 acgccggtgt cgacaacggc ggcgacgacg ccctggccgg tatagccggc ggcgtgggct
     3421 tcattgacgc tgtggatgtc ggccgccttg actgaatcga tagcctgcgg ccggatggtg
     3481 tcgggccgcg cggattcgac ggccggcggc ggattatcgg ccttgacctg cgccatgacc
     3541 gctgccttgc cgccgcgagc cagcagcgcg gccgcggctt cggaggtgat cagcgggtta
     3601 aacgtgtcgc cgcgcatccc ttccaggcct ggcgcgggca atggttggta ggtgttggtg
     3661 gaggtgacgg caatgacgcc gtcgacactg gccagccgga cgaggttgtt gattgccacc
     3721 tcgcccgtca cccattggac gtcaccgacg gggcgacttg tagccgagcg ctggagcaaa
     3781 tgggagacgg atgtccccgg ctggacgagg atgaaaacca tttggctttc cgcgtcaccg
     3841 ggacgatcga ccagttgttt tagatcgggt gagagcttct gaattgcttc atccgcggcg
     3901 gatgaactgg tggtaatggg ctgacgcggc ctgttggctt gcgtgagacg ggtagggacg
     3961 ccatcgccgg acgaggggtc ggactggggc gcggcagata gcggcaggga gaacaggaaa
     4021 accagaagga aaagaaggag caatgttata ccggaacgtc gtaatttgtc ttgcatcata
     4081 gcgccctcc
//
LOCUS       DAOMQG010000321         4074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9124_length_4074_cov_9.059965, whole genome shotgun sequence.
ACCESSION   DAOMQG010000321 DAOMQG010000000
VERSION     DAOMQG010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9124_length_4074_cov_9.059965"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLC06_13785"
     CDS             <1..879
                     /locus_tag="PLC06_13785"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HRO91563.1"
                     /translation="PSTACKVQDYLGASRAGAFDLSAACSGFVYALQMASQSIATGSV
                     RHAIVIGSETMSRVIDWQDRGTCVLFGDGAGAVVLSGSSVPGGVMATTLRSDGSGADL
                     LSLPALYHNPVPIVGAEFSSNGHHKNIIDMDGRQVYRFATNVIASSVQDVIRQAGLTI
                     DDIALIIPHQANTRIIEAAAKKLKLPMEKFYLNVEHNGNTSAASIPLALCDAVREGRL
                     RPDDNVVFVGFGGGLTWAASVVKWDVTPPEISFADREWRRVRYIMARGRSRFRRWQRR
                     VEAAMVRASNGKDSKN"
     gene            complement(876..1577)
                     /locus_tag="PLC06_13790"
     CDS             complement(876..1577)
                     /locus_tag="PLC06_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91564.1"
                     /translation="MSDLPNSPGSISFDEETWRQMPEFLGNLPEPVCLHIWGDESAGA
                     AEREAARLGRLFAEHFPERLSYRLLPRRINYSYYPVFGIMAGTAEESTDPGLRIIGLP
                     IGLQMTSLIAAIQAVAFRGQTLEPLSRLKLHQMMASSPEEIAIELLTSAEDEAGAVVA
                     KAAFGVAASTQRIKAFLIVTDFFPEAAQQYSAAYLPHTVINKRIHFNGELEEEELVRQ
                     IGLAVRRAIDTSRNG"
     gene            complement(1574..>1822)
                     /locus_tag="PLC06_13795"
     CDS             complement(1574..>1822)
                     /locus_tag="PLC06_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91565.1"
                     /translation="FFRVLLHDPQRQLPPEMLPRLFDLADVSHGGFASLRGMDLGLVF
                     TRHVAEQHNGRVTAVCSPGYGTSLALYLPASEPTPINR"
     assembly_gap    1823..1922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1923..2230)
                     /locus_tag="PLC06_13800"
     CDS             complement(<1923..2230)
                     /locus_tag="PLC06_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRO91566.1"
                     /translation="MTDETGSASNDLEKDEELTYLRRELAGLQEQLHRTQHRKSSLLA
                     MAAHDLRTPLAIIQGFAQLLEGELSANDNPAATEYVTNILAHSDSLNHMIENLVALDQ
                     "
     gene            complement(2280..3854)
                     /locus_tag="PLC06_13805"
     CDS             complement(2280..3854)
                     /locus_tag="PLC06_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91567.1"
                     /translation="MDDTADTGRFDPMPDPTEGNEASLSSIRRLLFATETERINQLES
                     EKSRLEADIADLQTRLNLLQADMAAAEVRLREQTEALAAGIDDVIARKAEEAPDDMAE
                     ALGGVLAGAVRVQERRSREDLVDAVGPVVSEAIQVQMHESRQSLVEALFPIIGELAQR
                     YIGEFFRELQRNIDGRLRSSFSPRRIMRRAKARLRGVEMADLELRDALPFNIRELFLI
                     QSGSGLLLARAGSNATLDSDLISGMLTAVRSFMHDSFKRAELPEYMDEIQYGEQRIII
                     QDGRLCYLAVVINGIEPPGFRGELHRFLNRLHANQHEVLKNFSGDMSELGDISQSTDR
                     FAVDLQALASSEKGPQPLTRGQKTVLALGGLGGVLLLGLACFYLQFTLALLPVAFGEP
                     SPTATWTSTPTTTQTPTATTTPTATAAPTRTPAPTATVTSMPTATERPTIEPSATPIP
                     FTVVTNRPVWAFSEPDLASAQVAIIDAGAPVTVITFEYPWLLVEWQSSEGLRQGWLTL
                     QWVDISGTPPPDLSFP"
     gene            complement(3869..>4074)
                     /locus_tag="PLC06_13810"
     CDS             complement(3869..>4074)
                     /locus_tag="PLC06_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91568.1"
                     /translation="EANERFRTLEEDARRRDDDLRAELLTVSAWLEDKKTSRHDLGQM
                     LEEIGLKLQTAATANANQSGDEG"
BASE COUNT          774 a         1125 c         1235 g          840 t
ORIGIN      
        1 ccttcgacgg cctgcaaggt gcaggactat ctgggagcaa gccgggcggg ggcgtttgat
       61 ctgagcgcgg cctgttccgg cttcgtctac gcgcttcaga tggcctctca atccatcgcc
      121 accggctcag tgcgtcatgc cattgtgatc ggttcggaga cgatgtcgcg cgtcatcgac
      181 tggcaagatc gtggcacgtg cgtcctgttc ggagatgggg ccggcgcagt ggtgttgagc
      241 ggctccagcg tgcccggcgg ggtgatggcc acaacgttgc gatcggatgg ctcgggcgcg
      301 gatttgctct cgttgcccgc cctctatcat aacccggttc ccattgttgg agccgaattt
      361 tcgtccaacg gccaccacaa gaacatcatt gatatggacg gccgccaggt ctatcgcttc
      421 gccaccaacg taatcgcctc ttcggtacag gatgtgatcc gccaggccgg tctgactatc
      481 gacgatattg cgctgatcat cccccatcag gccaacacgc gaatcatcga agctgcggcc
      541 aagaagctca agttgccgat ggagaagttc tatctgaacg tggagcacaa cggcaacaca
      601 tcggccgcgt caatcccgct ggctctttgc gacgccgtgc gcgaggggcg attgcgaccg
      661 gatgacaacg tggtttttgt ggggtttggc ggcggtctca catgggcggc gtccgtggtc
      721 aagtgggatg tgaccccgcc ggaaatatcg ttcgccgatc gcgaatggcg gcgggtgcgt
      781 tatatcatgg cgcgcggccg atcgcgattt cgccgctggc agcggcgggt cgaagcggcc
      841 atggtgcggg catccaacgg caaggattcg aagaactaac cgtttcgtga cgtgtcgatg
      901 gcccgccgaa ccgccagccc aatctgccgc accaactctt cctcttccaa ctccccgttg
      961 aagtgaattc gcttgttgat gaccgtgtgg ggcaggtagg cggccgaata ttgctgagct
     1021 gcctcgggga agaagtcggt tacgatcaga aaggccttga tgcgttgggt ggaggcggcc
     1081 acgccaaagg ccgccttggc cacgaccgcg cccgcctcat cttcggccga cgtcagaagc
     1141 tcgatagcga tctcttccgg agaggaggcc atcatctgat gcaactttag tcgtgacagc
     1201 ggctccagcg tttgcccacg aaaagccacg gcctggatcg cggcgatcag cgaagtcatc
     1261 tgcaagccga tcggcaagcc gatgatgcgc aatcccgggt cggtggattc ttcggccgtc
     1321 cctgccatga tgccgaacac aggataataa gaatagttga tccggcgggg tagtagacga
     1381 taggacaggc gctcgggaaa atgttcggca aacagtcgtc ccaggcgcgc tgcctctcgc
     1441 tcggccgcgc cggcagactc atcgccccag atatggagac aaacaggctc cggcaggttg
     1501 cccaggaatt ccggcatctg tcgccatgtt tcctcatcaa aagatataga gccgggagaa
     1561 ttgggtaggt cgctcatcgg tttatcgggg tcggttcact cgccggtaaa tagagggcca
     1621 acgatgtgcc atatcctggg gaacagactg cggtcactcg accgttatgt tgctcagcca
     1681 cgtggcgagt aaacaccaac cccagatcca ttccgcgcaa cgatgcgaaa ccgccatgac
     1741 tcacatcggc caggtcgaat aatcggggta gcatttcggg cggcaattgt cgttgggggt
     1801 cgtgtagcag gacgcggaag aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnatctggtc aagcgcgacg agattttcga tcatgtgatt cagcgagtcg gaatgagcca
     1981 ggatgttggt cacgtattcc gttgcagccg ggttatcatt cgcgcttaat tcgccttcca
     2041 gcaattgggc aaacccctgg atgattgcca acggcgttct aagatcgtga gcggccatag
     2101 ccaacaggct cgatttgcga tgctgcgttc gatgaagctg ctcctgcaag ccggccaatt
     2161 cgcgtctgag ataggtgagt tcctcatctt tctcaaggtc gttgcttgcg gaacctgttt
     2221 cgtcggtcat gagtgtccag ccgttgacat aaattgctca ttcttgccgc ttgcgtgaac
     2281 taagggaacg ataaatcggg tggaggagtc ccggagatat caacccattg gagcgtcagc
     2341 caaccttgtc gcaggccctc gctcgattgc cattctacca gaagccatgg atattcaaag
     2401 gtgatgaccg tcaccggcgc accggcatcg ataatagcga cctgtgccga cgccagatcc
     2461 ggctcgctga aagcccagac gggccggttg gtgacgacag tgaacggtat gggcgtcgcg
     2521 ctgggttcaa tcgtcggccg ctctgtggcc gtgggcatag atgtaacggt agccgtgggt
     2581 gcgggggtgc gggttggcgc tgccgtcgct gtcggtgtgg ttgtcgccgt aggcgtttgg
     2641 gtagtggtgg gtgttgaggt ccatgtggca gttggcgatg gttcaccgaa ggcgacgggc
     2701 agcaacgcca aggtaaattg caggtagaag caggccagcc ccagcaacaa tacccctcct
     2761 agcccgccca gagcgagaac cgttttctgg cccctggtga gcggctgggg gcctttctcc
     2821 gacgaagcca acgcctgcag atcaacggcg aaccggtccg tcgattgaga aatatcgccc
     2881 aattcggaca tgtcaccgct aaagttcttg agcacctcgt gctgattggc atgcaggcga
     2941 ttgaggaagc ggtgcagttc gccccgaaat cccggcggct cgatgccgtt aatcaccacg
     3001 gccagatagc acagccgtcc atcctggatg atgatccgtt gttcgccata ctggatctca
     3061 tccatatatt cgggcagttc ggctcgtttg aaggagtcat gcataaaact gcgcacggcc
     3121 gtcagcatcc cgctgatgag atcggaatcg agagtcgcat tgctgcctgc acgggccagc
     3181 agtaaaccgg agccggactg gatgagaaac agttcgcgaa tgttaaaggg cagcgcgtcg
     3241 cgcagttcca ggtcggccat ctccacgcca cgcaggcgag ccttggcgcg gcgcatgatc
     3301 cgtcttgggc tgaagcttga tcggaggcgg ccgtcgatat tacgctgtag ctcgcgaaag
     3361 aactcgccga tatagcgttg tgccagctcg ccgataatgg ggaagagcgc ttccacgagg
     3421 ctttggcgtg attcatgcat ctgcacctga atggcctcgc tcaccacagg gccgacggca
     3481 tctaccaggt cctcgcgcga tcggcgttcc tggacgcgaa ccgcaccggc caaaacaccc
     3541 cccagcgcct cggccatatc gtccggtgct tcctcggctt tgcgggcgat gacgtcgtcg
     3601 atgccggctg ccaatgcctc ggtttgctcg cgtaagcgca cttcggccgc ggccatgtca
     3661 gcctgcaaga ggttgaggcg cgtctgcaag tcagcgatat cggcttcgag acgggatttc
     3721 tcgctttcta attggttgat ccgctcggtt tctgtggcaa aaagaaggcg acggatagat
     3781 gaaagcgacg cttcgttgcc ttcggtgggg tcgggcattg gatcaaatcg accggtatcg
     3841 gcagtgtcgt ccatgacagt gccggttgct atccctcatc gccggattgg ttggcgttgg
     3901 ccgtggcggc ggtctgcagc ttgaggccga tctcttcgag catctgaccc aaatcatggc
     3961 gggaggtttt cttgtcttcc agccaggcgc tgaccgtgag caattcggcg cgcaagtcgt
     4021 cgtcgcggcg acgcgcgtct tcctcgaggg tgcgaaaacg ttcgttggcc tctt
//
LOCUS       DAOMQG010000322         4072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9129_length_4072_cov_5.670899, whole genome shotgun sequence.
ACCESSION   DAOMQG010000322 DAOMQG010000000
VERSION     DAOMQG010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9129_length_4072_cov_5.670899"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..426)
                     /locus_tag="PLC06_13815"
     CDS             complement(43..426)
                     /locus_tag="PLC06_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRO91569.1"
                     /translation="MSTPDIELLARGQALADDLRREGRNQDAEVLLSLLVVAEKQVDT
                     IRFLTTGQVASRVGVSRQTIVNWIKRGVIPGVQLGGRMVVPSSAMERFAPFEEILNEL
                     DDELPPLSPEDAARVAGADREDLRA"
     gene            complement(499..2352)
                     /locus_tag="PLC06_13820"
     CDS             complement(499..2352)
                     /locus_tag="PLC06_13820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRO91570.1"
                     /translation="MTYANIHVLVLDDDEAQGRRLAGPFAAWGWGVSHMSGDPRPALA
                     LLADVPFDLVLLDLRAAEREHYAFLRARRDDPRLSAIPVVITAWATAPLPRLARCIEL
                     GASDFIIHPDHPVLFRARLQNILQRKLMQDQAATALEAFNEIEKIADDLRLVILPIGA
                     ALSAEPEYDRLITRIVSEAQQVCNADAGALCLAQDDGLLHVLYSRVESLDFTWQSADG
                     PPEAIKPMPLTDEATGEPNFTSLPVFVALSGETVNVTDTLKSRFEVGRLYELLLGGRY
                     RPQSILAVPLRSGQVVGTLLLGDCRHPLNHEIVPFDAYHQQVAESLASQAAVVLNNRL
                     LNERQAQLMRFKRELEIGREIQRSFLPAQLPNPPGWELVARFNPALEVAGDFYDAFQL
                     PHGPLVLAIADVVGKGVTAALFMAIIRSLFRGLFQMNYFGVEQPAAEPEALSVLPPSM
                     PDGEALLNAVRLTNAYLIANHADTYSFATLFAAVLDPTTGRLLYVNAGHNPPLVLGAD
                     GRGGRELRDELSPTGPAIGLLLNTDYRLGEFELLPGDLLFAFTDGVIEARNPAAEELG
                     QDRLEALLTARDESAEATLAAVENAVLQHTGEAEAFDDVTMVGLRRAAVAE"
     gene            complement(2349..2804)
                     /locus_tag="PLC06_13825"
     CDS             complement(2349..2804)
                     /locus_tag="PLC06_13825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91571.1"
                     /translation="MNLEPLTIPGEPDYLSRLLDYVTWAAAAFGLDESATYRLALAVD
                     EIATNIVLHGYRDAGHRGDLTLWAESFADRLIVYLEDSGRPFDPREAPHPTDLDRPLE
                     ERRDGGLGIFLALWGVDSIHYEQVDGRNRTTFIMERPDETAGRSGPERR"
     gene            complement(2801..>4072)
                     /locus_tag="PLC06_13830"
     CDS             complement(2801..>4072)
                     /locus_tag="PLC06_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRO91572.1"
                     /translation="GNEKGPTISYRGLDNKTYYMLRPDGSYQNFSGTGNTLNCNNPVV
                     RNLVLDALRYWVAEYHIDGFRFDLASILGRDAEGNPLANPPLLESLAHDPVLGRTKLI
                     AEAWDAGGLYQVGTFPAYGRWAEWNGRYRDVVRRFLKGDEGLTGVMANMIKGSPHMYL
                     ERGPTASVNFITCHDGFTLKDLVSYAEKHNLANGEGNRDGANDNYSWNWGHEGPTDDP
                     VIVALRGRLMRNAVAILMVCQGVPMVHMGDEMAHTKYGNNNTYAQDNDLSWLNWDRLR
                     EHAGLFRFFSRCIAFRKAHPALRRRYHLHERDVIDSGFPDISWHGLREGKPDWSPHSR
                     TLAFMLDGPHAVLMPSQTADDHIYVALNMHWEPHTFELPRLQDGRRWHLFADTSRPSP
                     DDIHEPGQERLLRAQRNLPVGPRSVVILTGR"
BASE COUNT          715 a         1284 c         1378 g          695 t
ORIGIN      
        1 cctaactgat ctcacgcaga aacgcatcgg gagtgatgac tgtcacgccc gcaaatcctc
       61 acgatctgct cctgccactc tggctgcgtc ctcagggctt aatggaggca attcgtcatc
      121 cagttcattg aggatctcct caaacggcgc gaagcgttcc attgccgaag aagggacgac
      181 catgcggcca cccaactgga cgccggggat aacaccccgc ttgatccagt tgacgatggt
      241 ttgcctgctg acccctaccc gtgaggcaac ctgccctgtt gtcaggaacc ggattgtatc
      301 cacctgcttt tcggcaacca cgagaagaga gagtagaacc tcggcatcct gattccgccc
      361 ttcccgtcgc aggtcatcgg ccaaggcctg gccgcgcgct aaaagttcaa tgtccggtgt
      421 gctcatcaat cgtacctctt gaccaaatat agcacaaaaa ccacaaataa cacaaataac
      481 acaagctaca caaccgctct attcggcaac cgcagcacgc cgcagcccca ccatcgtcac
      541 atcatcaaac gcctccgcct cacccgtgtg ttgcagaacg gcgttctcga cggcggccag
      601 cgtcgcctcg gccgattcgt cgcgggccgt cagcagcgcc tccagccgat cctgacccag
      661 ctcctcggcc gccgggttgc gggcctcaat cacgccgtcg gtgaaggcga agagcagatc
      721 gcccggcagc agctcgaatt cgcccagccg gtagtcggtg ttgagcagca ggccgatggc
      781 cgggccggtg ggcgacagct cgtcgcgcag ttcccgcccg ccgcggccgt ccgcgcccag
      841 caccagcggc ggattgtgcc cggcgttgac gtagagcagc cggccggtcg tggggtcgag
      901 tacggcggcg aagagggtgg cgaaggagta ggtgtcggca tggttggcga tgagataggc
      961 gttggtcagg cgcacggcgt tgagcagcgc ctcaccgtcg ggcatggatg gcgggagaac
     1021 ggagagcgcc tccggttcgg ccgccggctg ttcaacccca aagtagttca tctggaacag
     1081 gccgcggaac aggctgcgaa tgatggccat gaacagggcg gcggtgaccc ctttgccgac
     1141 cacgtcggcg atggccagca ccaacgggcc atggggcagc tggaacgcgt cgtagaagtc
     1201 gcccgcgact tccagcgccg gattgaagcg ggccaccagc tcccagcccg gcgggttggg
     1261 gagttgggcg ggcaggaagc tgcgttgaat ctcgcggccg atctccagct cgcgcttgaa
     1321 gcgcatgagt tgggcttgcc gctcgttgag caaccggtta ttgagcacga cggccgcctg
     1381 cgaggccagc gattcggcca cctgctgatg ataggcgtcg aacgggacga tctcgtggtt
     1441 cagcgggtgg cgacaatcgc ccagcagcag cgtgcccacc acctgcccgc tgcgcagcgg
     1501 aacagccagg atggattggg gccggtagcg gccgcccagg agcagctcat agagtcgccc
     1561 cacctcaaag cggctcttga gcgtgtcggt cacgttcacc gtttcgcccg acagggcgac
     1621 gaagacgggc aggctggtga aattcggctc accggtcgct tcgtcggtca gcggcatggg
     1681 cttgatcgcc tccggcgggc cgtcggccga ttgccaggtg aaatccagcg attcgacccg
     1741 gctatagagg acgtggagca ggccgtcgtc ctgggccagg cacagcgcgc cggcgtcggc
     1801 gttgcacacc tgctgggcct cggagacgat gcgggtgatg agccggtcgt actccggctc
     1861 ggccgacagc gccgcgccga tgggcagaat gaccagccgc aggtcgtcgg cgatcttctc
     1921 gatctcgtta aacgcctcca gcgcggtggc ggcctgatcc tgcatcagct tgcgctgcag
     1981 gatgttttgc agacgggcac ggaacaacac ggggtggtcg gggtgaatga tgaagtcgct
     2041 cgcgcccaac tcgatgcagc gggccagccg gggcagcggc gcggtagccc aggcagtaat
     2101 gaccaccggg atggcgctca gccgggggtc gtcgcgccgg gcacgcagga aggcgtaatg
     2161 ttcgcgctcg gccgcccgca gatcgagcag caccaggtcg aacgggacgt cggccaggag
     2221 cgccagggcg ggacgcggat cgcccgacat gtggctgaca ccccagcccc acgcggcgaa
     2281 cggcccggcg aggcggcggc cctgcgcttc gtcatcatca agaaccagca cgtggatgtt
     2341 ggcgtaggtc atcggcgctc cggcccgctt cggccggccg tttcgtccgg ccgctccatg
     2401 ataaacgtgg tgcggttgcg gccgtccacc tgttcatagt ggatgctgtc taccccccac
     2461 agggcgagga agatgcccag gccgccgtcg cgtcgctctt ccagcggccg atcgaggtcg
     2521 gttggatgag gcgcctcgcg cgggtcgaac ggccggcccg aatcttccag atagacgatc
     2581 agccggtcgg cgaaagactc cgcccatagc gtcagatcgc cgcgatggcc ggcgtcccgg
     2641 tagccgtgga gaacgatgtt ggtggcgatc tcgtccacgg ccagggccag gcgataggtg
     2701 gccgattcat cgaggccaaa cgcggccgcc gcccaggtca cgtaatccag caagcggctc
     2761 agatagtccg gctctcccgg gatggtcaaa ggttcaaggt tcatcgaccc gtcaatatta
     2821 ccacggagcg cggcccgacg ggcaaattgc gctgggcgcg cagcagtcgt tcctggcccg
     2881 gctcgtggat gtcgtcgggc gacggccgcg aggtatcggc gaagaggtgc cagcggcggc
     2941 cgtcctgcag ccgcggcagc tcgaaggtgt ggggttccca atgcatgtta agggccacat
     3001 agatatggtc gtcggccgtc tggctgggca tcagcacggc gtgggggccg tcgagcatga
     3061 aggccagtgt gcggctgtgg ggcgaccagt cgggcttgcc ctcgcgcagg ccatgccacg
     3121 agatgtcggg gaagccgctg tcgatcacgt cccgctcgtg gaggtgatag cgccgccgca
     3181 gcgcggggtg ggccttgcgg aaggcgatgc agcggctgaa gaagcgaaac aggccggcgt
     3241 gttcccgcag ccgatcccag ttgagccagc tgaggtcgtt gtcctgggcg taggtattgt
     3301 tgttgccgta tttggtatgg gccatctcgt cgcccatgtg aaccatgggc acgccctggc
     3361 agaccatgag gatggcgacg gcattgcgca tcaggcggcc gcgcaaggcc acaatcaccg
     3421 ggtcatcggt cggcccctcg tggccccaat tccagctgta attgtcgttg gccccgtcgc
     3481 gattgccttc gccgttggcc agattgtgtt tttcggcata ggagacgaga tctttgaggg
     3541 tgaagccgtc gtggcaggtg atgaaattga ccgaagccgt cggcccgcgc tccagataca
     3601 tgtggggcga gcctttgatc atgttggcca tcacgccggt cagcccctcg tcgcccttca
     3661 ggaagcggcg caccacgtcg cgatagcggc cgttccattc cgcccagcgg ccgtaggccg
     3721 ggaaggtgcc gacctgatag aggccgcccg cgtcccaggc ctcggcgatc agcttcgtcc
     3781 ggcccagcac cgggtcgtgg gccagcgatt ccagcagcgg cgggttggcc agcgggttgc
     3841 cttcggcgtc gcgccccaga atcgaggcca ggtcaaagcg gaagccgtcg atgtggtatt
     3901 ccgccaccca gtagcgcagc gcgtcgagca ccagattgcg cacgacgggg ttgttgcagt
     3961 tgagcgtgtt gcccgtgccg ctgaagttct gatagctgcc gtcggggcgc agcatgtagt
     4021 aggtcttgtt gtccagcccg cgataggaaa tggtcggccc tttctcgttg cc
//
LOCUS       DAOMQG010000323         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9142_length_4068_cov_10.462248, whole genome shotgun sequence.
ACCESSION   DAOMQG010000323 DAOMQG010000000
VERSION     DAOMQG010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9142_length_4068_cov_10.462248"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1613
                     /locus_tag="PLC06_13835"
     CDS             51..1613
                     /locus_tag="PLC06_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRO91573.1"
                     /translation="MTQERRFKVTYSTLASPDPELHARYDQAVADFRADMGHTYPIFI
                     NGEERYNDKTFAKVSPTDTSMVMGYFQQGTAQDANDAVAAAKAAFPGWRDTPWQERVA
                     ILRRAADLLSDRLFKLGAIMSLEVGKNRLEALGDVEETADLIRYNCDAMEANNGFSKP
                     LKNESDKHHNRSVLKPYGVWVVISPFNFPGALAGGPSSAAMVAGNTVVFKPASDTAFT
                     GWLLTEAFRDAGVPAGVFNFVTGGGRSVGQTLVDHPDVAGITFTGSYDVGMNIIRTFA
                     GGKFPRPCVAEMGGKNPAIVSDKADLDKAAQGAMRSAFGLQGQKCSALSRIYVHKDVK
                     DEFMKKLVEQTAKINVGDPTDLNNWMGPVINESAYKDYQRFVEDLRQHGNIVYGGKTL
                     DDKGYYVAPTIADNVPEDHALWKQEMFLPIVMVEGYDDINEAMKKANDVEYGLTAGFF
                     SEDEDEIQWFLDNIEAGVIYVNRGSGATTGAWPGYQSFGGWKGSGSTGKAAGSFYYVQ
                     QYMKEQSQTVVD"
     gene            1801..2724
                     /locus_tag="PLC06_13840"
     CDS             1801..2724
                     /locus_tag="PLC06_13840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRO91574.1"
                     /translation="MTTNSKVISMREAVAGYVADGCTIAIEGFTAFICFAAAHEIIRQ
                     HKRDLVLCRMTPDLIYDQMIAAGVASKLVFSYLGNPGVGSLHCIRRAIEQAIPRPLAI
                     EEYSHFGMVGRYIAGASNLPFFPLRSYLGSDMPAANDRIRFVDSPYGDGPIAVVPPLK
                     PDVSFVHAQRADIHGNTHLWGLLGMQKEVAFASEKVIVVVEEIVDEDVIRRDPNRTLI
                     PGLIVDAVVHEPYGAHPSYVQGYYDRDNDFYLKWDESSRKQDTTEAWLQEWVYGLPDR
                     AAYLEKLGHEILERLDPGEFMADPVNYGRYG"
     gene            2738..3940
                     /locus_tag="PLC06_13845"
     CDS             2738..3940
                     /locus_tag="PLC06_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91575.1"
                     /translation="MPKLDLFSTDYLRLVLEIHAHHIDGYVDAYYGPEALKTEVEASL
                     AKSSAELLDAVADLQLRIPTTDPARQAYLTAGLRGIECTVRMLNGETIDYLDEVYRIY
                     DIRPELVDEARFTEAHKELDRALPAGKQGDSLAARLEAWRKTFEVETDKALPLLDLAR
                     VETRRRALAITDLPDDESVEISLVKGQPWSAYNWYLGNGRSLIEFNTDMPLQATALIG
                     TFAHEGYPGHHTEHLLKEKALYRERGYAEQAAMLLHSPAAVIAEGIATIALEMIFPEG
                     DHHEWNVERLLPAAKIAATPDMPEQLRRISNAGGDLRYVTGNVAILYHTGKLDREQAI
                     DYIRTYGLVTAERAAKSFSFLSHPLYRSYIFTYSVGYSLIKATADPKQTFRRLLTQQL
                     LPSQLATS"
BASE COUNT          892 a         1284 c         1141 g          751 t
ORIGIN      
        1 gcctgttcat ttccacccta tcccacataa ctatttccga ggagatccta atgacccagg
       61 aacgcagatt caaggttact tattcgacgc tggcaagccc cgatcccgag ctacatgcgc
      121 gctatgatca ggccgtggcc gactttcggg ccgatatggg ccacacctac ccgatattta
      181 tcaacggtga ggaacgctac aacgacaaga ccttcgccaa agtcagcccc accgacacta
      241 gcatggtcat gggctacttc cagcagggca cggctcagga cgccaacgac gccgtagccg
      301 cggccaaggc ggccttcccc ggctggcgcg atacgccatg gcaagagcga gtggctatcc
      361 tgcgacgcgc agccgatttg ctcagcgatc gcctgttcaa gctcggcgcg atcatgagcc
      421 tcgaagtcgg caagaaccgg ctggaagccc tcggcgatgt tgaggaaacg gccgatctga
      481 tccgctacaa ctgcgacgcg atggaagcca acaacggctt cagcaaaccg ctgaagaacg
      541 agagcgacaa gcatcacaac cgcagcgtcc tcaagcctta cggcgtatgg gtggtcatct
      601 cccccttcaa cttccccggc gcactggcgg gcggccccag cagcgcggcc atggtagccg
      661 gcaacacagt ggtcttcaaa cccgcgtcgg acacggcctt caccggctgg ctgctgaccg
      721 aagccttccg cgatgcgggc gttccggccg gtgtcttcaa cttcgtcacc ggcggcggcc
      781 gcagcgtagg ccagaccctg gtcgatcatc ccgatgttgc cggcatcacc ttcaccggca
      841 gctatgacgt cggcatgaac atcatccgca ccttcgccgg cggcaaattc ccgcggccgt
      901 gcgtggccga gatgggcggc aagaacccgg ccatcgtcag cgacaaggct gacctggaca
      961 aggcggccca gggcgcgatg cgttcggctt tcgggctgca aggccaaaag tgttcagcgc
     1021 tttctcgtat ctacgtccac aaggacgtca aggacgagtt catgaagaag ctggtggagc
     1081 aaacagccaa gatcaacgtt ggcgacccga ctgacctgaa caactggatg gggccggtca
     1141 tcaacgagag cgcctacaaa gactaccagc gctttgttga agacctgcgc cagcacggta
     1201 atatcgtcta tggcggaaag acgctggatg acaaaggcta ttacgtagcc ccgaccatcg
     1261 ccgataacgt gccggaagat catgcgctct ggaaacaaga gatgttcctg cccatcgtca
     1321 tggtggaggg gtacgacgac atcaatgaag ccatgaagaa ggccaacgat gtggaatatg
     1381 gcctcacggc gggcttcttc agcgaggacg aagacgagat tcagtggttc ctcgataaca
     1441 tcgaggccgg cgtcatctac gtcaatcgcg gcagcggcgc aaccaccggc gcgtggcccg
     1501 gctaccagtc cttcgggggc tggaaaggca gcggctccac cggcaaggcc gccggcagct
     1561 tctactacgt ccagcagtac atgaaggaac agagccagac ggttgttgat taagcctgca
     1621 tccggtcggt aggtcagtat tcagtagatc agtagatcag tattcagtgg gtcagtcaga
     1681 ggtgcgacca gataaccgca ctgacccact gacccactga cccactgacc cactgaccca
     1741 ctgatcgcac tgaccgcact gacccactga ccgcactgac ccactgaccc actgacccac
     1801 atgaccacca acagcaaagt catatccatg cgcgaggcag tcgccggcta cgtggcagac
     1861 ggctgcacga tcgccatcga gggcttcacc gcgttcatct gcttcgcggc cgcccacgaa
     1921 attattcgcc agcacaagcg cgatctcgtg ctttgccgca tgaccccgga tttgatctac
     1981 gatcagatga tcgcggcggg agtcgcctcg aagctggtct tcagctatct tggcaacccc
     2041 ggcgtcggca gcctgcactg cattcggcgc gccatagagc aggctattcc ccggccgttg
     2101 gccatcgagg agtactccca tttcggcatg gtcggccgct acattgccgg cgcttccaac
     2161 ctgccctttt ttccgctacg cagctatttg ggcagcgaca tgccggcggc taacgatcgg
     2221 atccggttcg tcgacagccc ctatggtgat gggccgatcg ccgtcgtgcc cccactgaag
     2281 ccggatgtat cctttgtcca cgcgcaacgg gccgatattc acggcaatac gcatctgtgg
     2341 ggcctgttgg ggatgcaaaa ggaagtcgcc tttgcctcgg agaaggtcat cgtcgtggtc
     2401 gaagagatcg tcgatgagga cgtcatccgt cgcgacccga accgcacgct catccccggc
     2461 ctcatcgttg atgccgtcgt ccacgaacct tacggcgcgc accccagcta tgtgcagggc
     2521 tattacgatc gcgacaatga cttctatttg aaatgggatg agtcatcgcg caagcaggat
     2581 acgaccgaag cctggctgca ggaatgggtc tatggcctac ccgaccgggc cgcatatctg
     2641 gagaaactcg gccatgagat cctcgagcgc ctcgaccctg gtgagttcat ggccgatccg
     2701 gtcaattacg gccgctacgg ctgagtggag tgctcaaatg ccgaaactcg atctgtttag
     2761 cacggattat ctgcgtctcg tgctggagat ccacgcacat catatcgatg gctacgtaga
     2821 tgcgtactat gggccggaag cgttgaagac cgaagtcgaa gcgtcgctcg ccaaatcatc
     2881 ggccgaactt ttggatgccg tcgccgatct gcaattgcgc atcccgacca cggatccggc
     2941 acggcaagca tacctcacag ccgggctgcg tggcatcgag tgcacggtac gcatgctcaa
     3001 cggcgaaacg atcgactatc tcgatgaggt ctaccggata tacgatatcc ggcccgaact
     3061 tgtcgatgag gcgcgattca cggaagcaca caaggaactg gaccgcgctc tacccgcggg
     3121 caagcagggc gatagcctgg ccgcccgtct cgaagcatgg cgcaagacgt tcgaggttga
     3181 aaccgataaa gcgcttccct tgctggacct ggctcgtgtt gaaacgcgcc gacgcgcgct
     3241 cgccatcacc gacttgccgg atgatgagtc ggtcgagatc agcctggtga agggccagcc
     3301 atggagcgcc tataactggt atctgggcaa cggccgctca ctcatcgaat ttaataccga
     3361 catgccgctt caggcaacgg cgctcatcgg cacctttgcc catgaaggtt atcccggtca
     3421 ccacaccgag cacctgctca aggagaaggc cctctatcgc gaacgaggat atgccgagca
     3481 ggcggccatg ttgctgcatt caccagcggc cgtgatcgcc gaaggcatcg ctaccatagc
     3541 gctggaaatg atcttccccg aaggtgacca tcacgaatgg aacgtcgaac gcctgcttcc
     3601 tgcggcgaag atcgccgcaa cgccggatat gcccgagcag ctgcgccgca tatctaacgc
     3661 cggcggcgat ctgcgctatg tcaccggcaa tgtggccatc ctctatcaca ccggcaaact
     3721 ggatcgggag caggccatcg actacatccg cacctacggg ctggtaacgg ccgagcgggc
     3781 tgccaagagc ttcagctttc tgagccaccc gctctatcgc tcttacatct tcacttattc
     3841 cgtgggctat agcctgatta aggccacggc cgacccaaaa caaacattcc gccggttgtt
     3901 gacgcagcaa ctcttgccct cgcaactggc caccagttaa caggaaccat tcatggacga
     3961 atccaccaaa atatccccgt ccgaattgat gatcgtcacc gcctcgcgcg cgctggaagg
     4021 caaccgaacc gtcttcgtcg gcgtcggctt gcccaacatc gcctgcaa
//
LOCUS       DAOMQG010000324         4054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9183_length_4054_cov_10.246812, whole genome shotgun sequence.
ACCESSION   DAOMQG010000324 DAOMQG010000000
VERSION     DAOMQG010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_4054_cov_10.246812"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="galE"
                     /locus_tag="PLC06_13850"
     CDS             complement(<1..778)
                     /gene="galE"
                     /locus_tag="PLC06_13850"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRO91576.1"
                     /translation="MKILVTGGAGYIGSHTTLELLRAGHDVVVVDNLSNSHEEALRRV
                     ASLAGRPPKFHRVDLLDKAALTTIFDETPVEAVIHFAALKSVGESVAQPLRYYHNNVT
                     GTLNLLEVMDSHNVSRFVFSSSATVYGDPATMPITEDMPLSPVNPYGQTKAMVENILG
                     DLYRADDRWAIAILRYFNPIGADPSGRIGEDPNGVPNNLIPYIAQVAVGRLPHLPVYG
                     NNYPTPDGTGVRDYIHVVDLALGHLKALDRLRGETGLLVYN"
     gene            complement(830..1528)
                     /locus_tag="PLC06_13855"
     CDS             complement(830..1528)
                     /locus_tag="PLC06_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRO91577.1"
                     /translation="MSQSHVLYDLQQIDSEIRAKKQRLGEVLRLQKEPAALVTAREHA
                     QAAAADLQQWQTRHKNLTLEIASVIDKAKRSEDRLYSGVVKNTKELADLQHEVEALGR
                     RRATLEDDALLTMMEVDERKTAKAAGDAVVDKLAGEFTTASTSLRQEQQTLALHLNGL
                     LEKRVRQASMAQPALLKSYDDLSRQKNGLAVAGLQGNKCLGCRLTLSASVIRAADEGK
                     VVHCENCGRFLCPV"
     gene            1678..2724
                     /locus_tag="PLC06_13860"
     CDS             1678..2724
                     /locus_tag="PLC06_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRO91578.1"
                     /translation="MFELGEQPYSEADAEWDAFVAAHPRGSLLQTTNWARLKSRFGWT
                     SQRVWMRRDGRLVGGAQVLFRSVALGIIKLGYVPHGPLVDWHDPEQVDVLFNHIDQAA
                     YGRGAGIVKIEPRIWQDEMNQEEWEALYRRHGCVPSPDTIQPPRTVVIDLRPPEEEIL
                     ARMKQKTRYNIRLAEKKGITVREGTAEDLPAFNRLMRLTGERDNFGVHDSNYYRAAFE
                     IFAPHSAALWLAEFEGRPLAGVMVFASGSSAAYLYGASSNEERQRMPAYAAQWAAMRW
                     AKARGCITYDMWGVPDVPEEELEANFADRQDGLWSVYRFKRGFGGEVKRTVGAADRVY
                     NQLLHRLYSRQRGR"
     gene            complement(2789..3874)
                     /gene="cax"
                     /locus_tag="PLC06_13865"
     CDS             complement(2789..3874)
                     /gene="cax"
                     /locus_tag="PLC06_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HRO91579.1"
                     /translation="MKSTLAKIEYWIGLNPILLILALFIPAAIFLELTHADPLWILLV
                     SAAAIVPLAAMIGKGTEALAEKIGERMGGLLNATLGNAAELIIAIVALRKGLIDLVLA
                     SITGSILGNLLLIMGLSLLIGGIRHGIQRFNRENAGLDATLLVLAVFTLAIPSFFNRA
                     LEPDFTRVESLSIGASIAILVMYGLVILYSFTSKTHEGDPQAREAHAPTGWSTTHALV
                     VLVAAVGFIALLSEFLVTAVEPVTVELGLSEFFIGIILIPLVGNAAEHFVAVQVATKN
                     RMDLALSIAIGSSLQVALFVAPLLVFISLALGHPMPLEFSIYEILAVSAASLIAALVS
                     LDGKSNWLEGSMLLVLYVILAVAFFFT"
BASE COUNT          822 a         1163 c         1272 g          797 t
ORIGIN      
        1 ggttgtagac gagcaacccc gtctcgccgc gcaatctgtc gagcgccttg agatgtccca
       61 gcgccagatc gacgacgtgg atgtaatcgc gcacgccggt gccatcgggc gtggggtagt
      121 tgttgccgta gacgggcagg tgcggcaatc ggccgacggc cacctgggcg atgtagggga
      181 tcagattgtt gggcacccca ttcgggtctt cgccgatgcg gccgctggga tcggcgccga
      241 tgggattgaa atagcgcagg atggcaatcg cccaacggtc atcggcacga tacagatcgc
      301 ccaggatgtt ctctaccatg gctttggttt ggccgtaggg gttcacgggt gacaggggca
      361 tatcctcggt gataggcatc gtcgccggat cgccgtagac ggtggccgat gagctgaaca
      421 caaaccggct cacgttgtgg ctgtccatca cttccagcag gttgagcgtg ccggtcacgt
      481 tgttgtggta gtagcgcaac ggctgggcca cggattcgcc cacggatttg agcgcggcga
      541 aatggatgac ggcctcgacg ggagtctcgt cgaagatcgt ggtcagcgct gccttgtcca
      601 gcaaatctac ccgatggaac ttcggcggcc gccccgccag cgaagcgaca cggcgcagcg
      661 cctcctcatg gctattgctg agattgtcga cgaccacgac atcgtgcccg gcgcgaagca
      721 gttccagcgt ggtatggctg ccgatgtagc cggcgccgcc ggtgaccaga attttcatgg
      781 atcaagcctc ttgaggtggg gacgacgatg attgagcgac gccgcgggat tataccgggc
      841 agaggaagcg gccgcagttc tcgcaatgga cgacttttcc ctcatcggcc gcccgaatca
      901 cgctggccga gagggtaagc cggcagccga gacatttgtt tccctgtaga ccggccaccg
      961 ccaggccgtt tttctggcga ctcagatcgt cgtagctctt cagcagagcc ggctgggcca
     1021 ttgacgcttg ccgcacccgc ttttccagca agccattcag atgcagcgcc agagtttgct
     1081 gttcctggcg caatgatgtc gatgccgtgg tgaattcgcc ggccagtttg tcaacaacag
     1141 cgtcgcccgc ggcttttgca gtcttgcgct cgtcaacctc catcatcgtc agcagggcgt
     1201 cgtcttccag cgtggcccgc cgtcgcccca gcgcctccac ttcgtgctgc aaatcggcca
     1261 gttccttggt gttcttgacg acccccgaat agagccgatc ttcggagcgc ttggccttgt
     1321 cgatcaccga ggcgatttca agggtcaggt tcttgtgcct ggtctgccat tgttgcaggt
     1381 cggctgcggc cgcctgggcg tgctcgcgag cagtcaccaa cgcagcaggc tccttttgca
     1441 gtcgcaatac ttcgcccaat cgctgctttt tggcgcggat ctcgctgtct atttgttgca
     1501 ggtcatatag tacgtgtgac tgactcatgg atctgtttgc cctcttgtgg ggcgcattgt
     1561 agcacaatga ccgttattgg cgaagaggac ggatggcggc cgatcggtgc cggatgatgg
     1621 gctgaattgg gtgaaggcgg cgattgccct tataattcat cctctatggt tgcaaaaatg
     1681 tttgaattgg gcgagcagcc ctattcggaa gccgacgcgg aatgggatgc gttcgtggcc
     1741 gcgcacccgc gcggcagcct gctgcagacg acgaactggg cgcggctgaa aagtcgcttc
     1801 ggctggactt cacagcgcgt atggatgcgg cgcgacggcc gtctggtggg cggggcgcag
     1861 gtgctctttc gctctgtggc tctgggtatc atcaagctgg gctatgttcc ccatggtccg
     1921 ctggtggatt ggcatgatcc cgaacaggtc gatgtcctgt tcaaccacat cgaccaggcc
     1981 gcttacgggc gcggcgcggg catcgtcaag atcgaaccgc gcatttggca ggacgagatg
     2041 aaccaggagg aatgggaggc gctataccgc cggcatggtt gcgtaccttc ccccgacacc
     2101 atccagccgc cgcgcacagt ggtgatcgat ctgcggccgc cggaagaaga gattctggcc
     2161 cgcatgaagc aaaagacgcg ctacaacatt cggctggcgg agaagaaagg catcactgtt
     2221 cgcgagggga cggccgaaga tctgccggcc tttaatcgat tgatgcggct gacgggcgag
     2281 cgcgataatt tcggcgtcca tgactccaat tactaccgcg ccgctttcga gatatttgcc
     2341 ccccacagcg cggcactatg gctggccgag ttcgaagggc gtcccttggc gggggtgatg
     2401 gtctttgcgt cgggcagttc ggccgcctat ctctatggcg cgtcgagcaa tgaggagcgc
     2461 cagcgcatgc cggcctacgc ggcacaatgg gcggccatgc gctgggccaa agcccgcggt
     2521 tgcatcacct atgacatgtg gggggttccc gatgtcccgg aagaggaact ggaggcgaat
     2581 ttcgccgacc ggcaggatgg cctatggtcg gtctaccggt tcaagcgggg atttggggga
     2641 gaggtcaagc gcaccgtggg cgcggccgat cgcgtttata atcagctgct ccatcggctc
     2701 tatagccggc aacgtggccg ctaatagata gcgcaagctg ccggcatgtc gcccggttga
     2761 ccggcagctt gcgctatagc ttcctgcttt atgtgaagaa gaacgccaca gccaggatga
     2821 cgtagaggac cagcagcatt gacccttcca gccagttgct ttttccatcc agcgagacca
     2881 acgcggcgat aagcgaagcg gccgagacgg ccaggatctc gtaaatcgag aactccagcg
     2941 gcatcggatg gcccagcgcc aggctgatga agaccagcag cggcgcgacg aacaacgcaa
     3001 cctgcaagct ggagccgatg gcgatgctca gggccagatc catccgattc ttggttgcca
     3061 cctggacggc gacgaaatgc tcggcggcat tacctaccaa cgggatcagg atgatgccga
     3121 tgaagaactc gctcagaccc agctcaactg tgaccggctc gacggccgtg accaggaact
     3181 ccgacagcag cgcgatgaag cccacagccg caacaaggac gaccagcgcg tgggtcgtgc
     3241 tccagcccgt gggggcatgg gcttcgcgcg cctgcgggtc gccttcgtgc gtcttcgaag
     3301 tgaacgaata gaggatcacc agcccataca tgaccagaat ggcgatggac gcgccgatgc
     3361 tcagcgactc gacccgcgtg aaatcgggtt caagcgcccg gttaaaaaat gacgggatcg
     3421 ccagcgtgaa gacggccagc acgagcaaag tggcgtccag gccggcgttt tcgcgattga
     3481 accgctggat cccatgacga atgccgccga tcagaagcga cagacccata atgagcagca
     3541 ggttgcccag gatggagccg gtgatcgacg ccaggacgag gtcgatcaat cccttgcgca
     3601 aggcgacgat ggcaatgatc agttcggccg cgttgcccag cgtggcgttc aacaaaccgc
     3661 ccatccgttc gccgatcttt tcggccagcg cttcggtgcc cttgccgatc atggccgcca
     3721 acggcacgat agcggcggcc gagacgagca gaatccacaa tggatccgca tgagtcaatt
     3781 caaggaagat ggcggcgggg ataaacaagg ccagaataag gaggatggga ttaagcccaa
     3841 tccagtattc tatcttcgcc aaggtgcttt tcatcaataa accctccgga tatgatcaac
     3901 aaaaaatctg gcgaattgta gcaaaagcat tgccaggtaa cgaacagtta gcggcgtatt
     3961 ccctttatct gcggtgcatt accgataccg agttcgcaac aattcgaaat atgactcagt
     4021 cgggttcgag ttcaagctga acgaacataa atct
//
LOCUS       DAOMQG010000325         4014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9305_length_4014_cov_10.074009, whole genome shotgun sequence.
ACCESSION   DAOMQG010000325 DAOMQG010000000
VERSION     DAOMQG010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_4014_cov_10.074009"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLC06_13870"
     CDS             complement(<1..964)
                     /locus_tag="PLC06_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRO91580.1"
                     /translation="MTTPDFKQEAAALSGDLIRVRRDLHRHPELGFQETRTAALVAET
                     LTRLGLEVQTGVGQTGVVALLEGARPGPAVLLRFDMDALPIQEESTHEYVSTNPGVMH
                     ACGHDGHVAMGLGLAHIFARRQAQMAGSLKFLFQPAEEGLGGALAVMAGGALENPRPD
                     VALAMHLWSPVHLGQARVVEGPAMASSSVFTLTVEGKGGHGAAPHLATDPIIAAAQIV
                     VALQSIVSRNADPHDNVVVSIGEFSAGTTFNVIPERAILKGTVRSYNKTTHRMVYRRI
                     LEMATNVATAFGCRATMETVAIVAAVVNAPEPTAVVRAAAERVLG"
     gene            complement(1079..1435)
                     /locus_tag="PLC06_13875"
     CDS             complement(1079..1435)
                     /locus_tag="PLC06_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HRO91581.1"
                     /translation="MDDNDKFDTEFLDEEERDLIESYERDEWIPVPNMEARIAEYQEA
                     ARTFFRKDRRVNIRISNRDVLALQEMAQEDGIPYQTLMSSILHKYVTGRLVERPRTMP
                     RHTELALHEEQEPYDS"
     gene            complement(1435..1719)
                     /locus_tag="PLC06_13880"
     CDS             complement(1435..1719)
                     /locus_tag="PLC06_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017293032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HRO91582.1"
                     /translation="MNNNFQWNEEKNRLLKEERGISFEEIVTHILQGDLLSIKEHPNQ
                     ERYPGQQMFIVRMDDYVYLVPFVQEDGVTFLKTIYPSRKETRHYLRGSNQ"
     gene            complement(1760..2104)
                     /locus_tag="PLC06_13885"
     CDS             complement(1760..2104)
                     /locus_tag="PLC06_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO91583.1"
                     /translation="MATYHAIKSWQVHYSDPIRGTAGDRLTLGRRDDEFPGWVWATAA
                     DGRAGWIPESWLCVEGDSGRLIRDYTAAELPLEPGDVVSGELVESGWLWATNAAGQRG
                     WAPLGCLQLINE"
     gene            complement(2104..>4014)
                     /locus_tag="PLC06_13890"
     CDS             complement(2104..>4014)
                     /locus_tag="PLC06_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91584.1"
                     /translation="LFVDEVHRWNKAQQDALLPHVENGTIILIGATTENPYFEVNKAL
                     VSRSRIFQLRPLTEDDLRAIAIQALNDPERGYGSLKIELQPAALDHLVDVANGDARGV
                     LNALELAVETTPPDESGVIRVDLDVAEESIQRRAVLYDKDGDVHYDTISAFIKSLRGS
                     DTDAALYWMAKMVYAGEDPRFIFRRMAIFAGEDVGMADPQAMGVVVNCWETFERIGMP
                     EGRYPLSLAAIYLSTAPKSNSAFAFFDALGVVEKEREADVPNHLKDGSRDKEGFGHGE
                     GYLYPHAYRNHWVAQQYLPETLQGKVFYQPGELGYERAIRDDVARRREAQLTAMVEGE
                     NETPGHLEILTTSPTNRTKDAWLQRTVASSGRQLGGLRDRLFEMAGVQRHHLILDANA
                     GSGLLTWEAVRRAPEGGVWALAADESAGEALRQQAARLPEVERPAILVGEPAELDYLL
                     SLRGETDVRFDCIMGRNTLSRPDRSSETCQVLANHLLPGGRLVLSQSIPRHTQRLYDL
                     IEWGRDTELRDKVIAAEETIYADPADPMTNWDEHDLRAALEAAGLTDIRLTVEEQTEE
                     RLITAAHLDRWFGAGAADGRPSYRERLAAGGVMPDEMEKIERLFRNQLLDRAVGWQSQ
                     LAYIRAGKSSEG"
BASE COUNT          662 a         1246 c         1302 g          804 t
ORIGIN      
        1 tgcccagcac gcgctcggcc gcggcgcgaa cgacggccgt cggttccggt gcgttgacca
       61 cggcggcgac gatggccacc gtctccatgg tcgcccggca accgaaggcg gtcgccacgt
      121 tggtggccat ctccaggatg cgccggtaga ccatgcggtg ggtggtcttg ttgtagctgc
      181 gcaccgtacc cttcaggatg gcccgttcgg ggatgacgtt gaaggttgtg ccggccgaga
      241 attcgccgat ggagacgacc acgttatcgt gcgggtcggc gttgcggctg acgatgctct
      301 gcagggcgac gacgatctgc gccgcggcga tgatcgggtc ggtcgccaga tggggcgcgg
      361 ccccgtggcc gcccttgccc tccacggtga gcgtgaagac gctggacgag gccattgccg
      421 ggccttcgac gacgcgcgcc tgtcccagat gcaccggcga ccagagatgc atggccaggg
      481 ccacgtccgg ccgcgggttc tccagcgcac ccccggccat gaccgccagt gccccgccca
      541 acccttcctc ggccggttgg aacaggaact tgagcgagcc ggccatttgc gcttgccggc
      601 gggcgaaaat gtgcgccaac cccaggccca tcgccacgtg gccgtcgtgg ccgcaggcgt
      661 gcatgacgcc gggattggtc gagacgtact cgtgagtgct ttcctcctgg atgggcaggg
      721 catccatgtc gaagcggaga agcacggcgg ggccggggcg cgcgccttcc agcaaggcaa
      781 cgacgccggt ctggccgacg ccggtctgta cctcaagccc cagcctggtc agcgtctcgg
      841 ccacgagggc ggccgtgcgc gtctcctgga agccgagttc cggatggcgg tgcaggtcgc
      901 ggcggacgcg aatcagatcg ccggagaggg cggcggcctc ttgtttgaag tcgggagttg
      961 tcatctccct ctatggtaga cgtggaatat tacgtccggc gttacacgcg cgtgcggatt
     1021 attgaagggg ggcacatttt agacacgatg tttcgcgctg tcgcccggaa taatgcgttt
     1081 aactgtcgta cggctcttgc tcttcatgaa gcgcaagctc tgtgtggcgc ggcatcgtcc
     1141 gcggccgctc caccagccgt ccggtgacgt atttgtggag gatgctggac atgagggtct
     1201 ggtaggggat gccgtcttcc tgggccattt cctggagggc caaaacatcc cggtttgaga
     1261 tgcggatgtt gacgcggcga tccttgcgga agaaggtgcg ggcagcttcc tgatattcgg
     1321 cgattcgagc ttccatgttt ggaacgggaa tccattcgtc cctttcataa gattcaatga
     1381 gatcgcgctc ttcttcatcc aggaattctg tatcgaactt atcgttgtcg tccattattg
     1441 attcgatccc ctgagataat gacgcgtttc tttcctgctg ggatagattg ttttcagaaa
     1501 tgtaacgcca tcttcctgaa caaatggtac gagatagacg taatcatcca tacgaacgat
     1561 aaacatctgt tggccggggt acctttcctg gtttggatgt tctttaatcg aaagcaggtc
     1621 gccttgaaga atatgggtca cgatctcttc aaatgaaatg ccacgttctt ccttcagcaa
     1681 ccggttcttt tcctcgttcc actggaagtt attgttcata aggtatcaca atgtgtgcct
     1741 aatggcaaca ccccgcagat tattcgttga taagttgcag acagcccaac ggcgcccacc
     1801 ctctctgccc tgccgcgttc gttgcccata accagccgct ctccacaagc tccccgctga
     1861 ccacatcgcc cggctccaac ggcaattcgg ccgccgtgta gtcgcggatg agccggccgc
     1921 tgtcgccctc gacgcacagc caactttcgg ggatccagcc cgcgcggccg tcggcggccg
     1981 tggcccagac ccagccaggg aactcgtcgt cgcggcggcc gagcgtcagg cggtcgccgg
     2041 ccgtgccgcg gatggggtcg gagtagtgga cttgccaaga tttaatggcg tgatacgttg
     2101 ccatcaaccc tcactggatt tcccggcgcg aatgtaggcc agttgcgact gccagccgac
     2161 ggcccgatcg agtaactgat tgcggaacag tcgttcgatc ttctccatct cgtccggcat
     2221 gacgcccccg gccgccaacc gctcgcgata acttggccgt ccgtcggccg cgcccgcgcc
     2281 gaaccagcgg tcaaggtggg cggcggtgat gagccgctcc tccgtctgct cctcgacggt
     2341 caggcggatg tcggtcagcc cggcggcctc cagcgcggcc ctcagatcgt gctcgtccca
     2401 gttggtcatc gggtcggccg ggtcggcgta gatggtttcc tcggcggcga tgaccttgtc
     2461 gcgcagttct gtgtcgcggc cccattcgat caggtcgtag aggcgttgcg tgtggcgggg
     2521 gatggattgg gagaggacga gacggccgcc gggcagcagg tgattggcca agacctgaca
     2581 ggtctcagaa gacctgtcag gtctggatag cgtgttgcga cccatgatgc aatcgaagcg
     2641 cacatcagtc tcgccgcgca gggacaacag atagtcgagt tcagccggct cgccgacgag
     2701 gatggccggt cgctcgacct ccggcaggcg ggcggcctgc tggcgcagtg cctctcccgc
     2761 agattcatcg gccgccaacg cccacacgcc gccttccggc gcgcgccgca cggcctccca
     2821 cgtgagcagc ccactcccgg cattggcgtc gaggatgagg tgatggcgct gcacaccggc
     2881 catttcgaac aaccggtcgc gcaggccgcc gagttggcgg ccgctgctcg ccaccgtgcg
     2941 ttgcagccag gcgtccttgg tgcggttggt cgggctggtg gtcaatattt ccaagtgacc
     3001 gggtgtttcg ttctcgcctt cgaccatcgc cgttagttgt gcctcgcgcc gtcgggccac
     3061 atcgtcgcgg atggctcgct cgtaacccag ttcgcccggc tggtagaaga ctttcccttg
     3121 cagcgtctcg ggcagatact gctgagccac ccagtggttg cgataggcat ggggatagag
     3181 atagccctca ccgtggccga agccctcctt gtcgcggctg ccgtccttca gatgattggg
     3241 cacgtcggcc tcgcgctctt tctccaccac gcccagcgcg tcgaagaagg cgaaggcgga
     3301 gttggacttg ggcgcggtgg acagatagat ggccgccagc gacagtggat agcgcccctc
     3361 cggcatgccg atgcgctcga atgtctccca gcagttgacg acgacgccca tcgcctgcgg
     3421 gtcggccatg cccacgtcct ccccggcgaa gatggccatg cgccggaaga tgaagcgcgg
     3481 gtcctctccg gcatagacca ttttggccat ccagtagagc gcggcgtcgg tgtcggagcc
     3541 gcgcaggctc ttgatgaagg cggagatggt gtcgtagtga acgtcgccgt ccttgtcata
     3601 gagcaccgcc cgacgctgga tcgactcctc ggccacgtcg agatcgacgc ggataacgcc
     3661 gctctcgtct gggggggtcg tctccacagc cagctccagc gcgttgagca cgccgcgggc
     3721 gtcgccgttg gccacgtcga ccaggtgatc cagtgctgcg ggctgcaatt caatcttcag
     3781 gctgccgtag ccgcgttcgg gatcattgag agcctggatg gcgatggccc gcaggtcgtc
     3841 ttcggtcagc ggccggagct ggaagatgcg gctgcggctg acgagtgcct tgttgacctc
     3901 gaagtaggga ttctcggtcg tcgcgccgat gaggatgatc gtgccgttct ccacgtgggg
     3961 cagcaaggcg tcctgttggg ccttgttcca gcggtgcacc tcgtcgacaa agag
//
LOCUS       DAOMQG010000326         3972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9441_length_3972_cov_9.990809, whole genome shotgun sequence.
ACCESSION   DAOMQG010000326 DAOMQG010000000
VERSION     DAOMQG010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9441_length_3972_cov_9.990809"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLC06_13895"
     CDS             complement(<1..694)
                     /locus_tag="PLC06_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRO91585.1"
                     /translation="MLIGLIVFVATLFQAFLGFGGALIAMPLLVMVIGIQTATPVFAL
                     VGTLATLLNAVRLRAYTTPRDLARLVVPAIVGVPLGILLLARVDSELITAALGVVLIG
                     YSLYNLLGLTMPSLAHPAWAYLAGFSSGVFSGAFNTGGPPVIVYATARQWPADQFRGN
                     LQTYFMIVSVFLVVGHGLSGHLTADVWRMALPAVPALLAGQAIGVYLCRYVGADFFRK
                     IVLVFLLILGIQL"
     gene            complement(694..1545)
                     /locus_tag="PLC06_13900"
     CDS             complement(694..1545)
                     /locus_tag="PLC06_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRO91586.1"
                     /translation="MATETIWQLYPMPAQEVDLTGLYLRHDLRRIAEGRERPFVYTNY
                     VTSIDGRIAVPRPGGGGMMVPKATANDRDWRLFQELAVQADVIISSGRYLRDYAEGKA
                     QEILRIYDEPRFADLGQWRARRGLPAYPDLAIVSGSLDFSIPPFLTQSGRKVVVITHG
                     AADASRRRRLEQETEHVIVAGEKDVSGRQAIDALGGYGYRTIYMATGPQVHHLLLVDN
                     MIDRLYTTVVHRLLGGQPFSSLVEGELLSPPVDMHLNSVYFDPHAFERIDHATDRVGQ
                     QFICYER"
     gene            complement(1545..2369)
                     /locus_tag="PLC06_13905"
     CDS             complement(1545..2369)
                     /locus_tag="PLC06_13905"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO91587.1"
                     /translation="MRYAIRRISPMGALLYGLLFGLGAWLIPGAFLGWLASAAVQGAR
                     TWLNGLQVTIPMPVGEGITLDLVSALSLGDTQSRLAALAGQELTLTLAVALGTIAAGM
                     LLTGLTASFGAILYNLFAGVFGGVEVRLDALDVTARRSVPPGVPAAARPMAASSQATV
                     PRPRVEPPQVARPPATAWLTAANGGERRALRADVTRIGSAPGNDIVLPGLAPQHAEIR
                     RESDRYLIYDLGSRRTWVNDTQVAAVHMLKDGFRLQMGQSEFIIHLPTNHQAIAEG"
     gene            complement(2434..3141)
                     /locus_tag="PLC06_13910"
     CDS             complement(2434..3141)
                     /locus_tag="PLC06_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91588.1"
                     /translation="MTEATPTATKPPTAVATITPLPTIMGIIITPTIAFGPEVNFIQN
                     GDFADDWANGWTLESRGETAEVAIGPATDDPQTRSLHLSRSGPGMSWLAQRVVLTFPV
                     EGLMFRGHFRLSGTAKGDAEGRAFILLRYEDANGAPVAMTVWLDDAAVDTDLWGTAPL
                     PEQGSVISEHSVREDWQRVELPLAQEFAGALSDADMESVRQITVMMGVVGGESCPAAG
                     CEAMLEVAELSLTAETP"
     gene            complement(3653..>3972)
                     /locus_tag="PLC06_13915"
     CDS             complement(3653..>3972)
                     /locus_tag="PLC06_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO91589.1"
                     /translation="RLLVMPAGNVLSLIPGQDTYIIGRIDPASGSYPDVDLVPFGGEE
                     GGVSRRHAQISRSGDQFFIEDLNSVNYTFLNKEKLAAEAIQPLQDGDEIRLGRVLLRF
                     KTA"
BASE COUNT          844 a         1223 c         1215 g          690 t
ORIGIN      
        1 tcaactgtat acccagaatc aacaggaaga ccagcactat cttgcggaag aaatctgctc
       61 ccacatagcg gcaaaggtag acgcctatcg cttgaccggc cagcaaagcg ggcacggcag
      121 gcagcgccat tcgccacaca tcagccgtaa gatggccact caggccatgt cccaccacca
      181 gaaagacgct gacgatcata aagtaggtct gaaggttgcc ccgaaattga tcggccggcc
      241 attgtcgggc ggtagcatag acaatgaccg gcgggccacc ggtgttgaag gcgccactga
      301 aaacacccga actgaagccg gccaagtagg cccaggccgg atgagccaag gagggcatcg
      361 tcaatcccag gaggttatag agggaatagc cgatgaggac gaccccaagc gcggcagtaa
      421 tcagctccga atcaacgcgg gcaaggagca ggatgcccaa gggaacgccc acgatggcgg
      481 gcactaccag tcgtgccagg tcacgcggcg tggtgtaggc gcgcaagcga acggcgttca
      541 gcaaagtcgc cagtgtgccc acgagcgcga agaccggcgt ggccgtttga atcccgatca
      601 ccatgaccaa cagaggcatg gcgatcagcg ctcccccaaa gccaagaaag gcctgaaaaa
      661 gcgttgctac gaagacgatc agcccgatca gcatcaccgc tcgtagcaga taaattgctg
      721 ccccacgcga tcggtggcgt ggtcgatgcg ctcgaacgca tgaggatcaa agtaaaccga
      781 gttaagatgc atatcgaccg gcgggctgag caattctcct tcaaccaggc tgctgaaggg
      841 ttggccgccc agcagtcgat gaacaaccgt ggtgtaaagc cgatcgatca tattgtcgac
      901 cagcagcaag tgatggactt gcgggccggt agccatgtag atcgtccggt agccatagcc
      961 gcccagggcg tcgatcgcct gccggcccga aacatccttc tcgccggcca caatgacgtg
     1021 ctcggtctcc tgctccagcc tgcgcctgcg tgatgcgtcg gccgcgccgt gggtgatgac
     1081 gacgaccttt cggcccgact gcgtcaggaa gggcgggatg gagaaatcga ggctgccgct
     1141 gacgatggcc agatcgggat atgcgggcaa tccccgcctg gcacgccatt ggcccaggtc
     1201 cgcgaagcgc ggttcgtcgt agatgcgcag aatctcctgg gcctttcctt cggcataatc
     1261 gcgcaggtaa cggccgctgc tgatgatcac atcggcctgg acggccagct cctggaacag
     1321 ccgccagtcc cgatcattgg ccgtggcctt cggcaccatc attcccccgc ctcccggccg
     1381 cggaacagcg atacgaccat cgatgctggt gacgtaattg gtgtagacaa agggacgttc
     1441 ccgaccctcc gcaatccgtc gcaagtcgtg ccgcaggtaa agccccgtta ggtcaacctc
     1501 ttgcgcgggc atcggataga gttgccagat cgtctcggtg gccattatcc ctccgcaatc
     1561 gcctgatgat tcgtaggaag atgaatgata aattcggatt gccccatttg cagccgaaag
     1621 ccgtccttga gcatatgtac cgccgcgacc tgggtgtcat taacccacgt gcgccggctg
     1681 ccgagatcgt agatgagata gcggtcgctc tcgcgccgga tctcggcgtg ctgtggagcc
     1741 aggccgggca atacaatatc gttgcccggc gcgctgccga tccgggtgac gtcggcgcgg
     1801 agcgcccgcc gttcaccgcc attggccgcc gtcagccagg ctgtggcggg gggccgggct
     1861 acctggggtg gctccacgcg cgggcgagga actgtcgcct gtgacgaggc ggccataggc
     1921 cgggcagcgg ccggcacacc gggcggcacg ctgcgacgag ccgtcacatc caacgcatcg
     1981 agccgaacct cgaccccgcc gaagacgccg gcaaacaggt tatagaggat cgcgccaaaa
     2041 gaggccgtca gccccgtcag cagcatgccc gcggcgatgg tgcccagagc cacggccagg
     2101 gtaagcgtca gctcctggcc tgccagcgcc gccaatcggg actgtgtatc gccaagggac
     2161 aacgccgaca ccaggtcgag cgtgatgccc tcgcccacgg gcatggggat cgttacttgc
     2221 agaccattca accaggtgcg agcgccctgg acggccgcgc ttgccagcca acccaggaaa
     2281 gcgccgggaa tcagccacgc acccaagcca aacagtaatc catacagcaa cgcccccatc
     2341 gggctgattc gtcttatcgc gtatcgcatc gggtcatctt atccaatatc ggggttcaat
     2401 aaatagtatc aaggcttgac aacagctcta tgactacggc gtttcggccg tcagtgataa
     2461 ctcggccact tccagcatcg cctcacaacc agccgccggg cacgattcac cgccgaccac
     2521 acccatcatc accgtgattt gccgcacaga ttccatatct gcgtcggaca gcgcgcccgc
     2581 gaattcctgg gccaacggca attcgacgcg ctgccaatcc tcgcgaacgg aatgctcgct
     2641 aatcacggaa ccttgttccg gcagtggagc agtgccccac aggtcagtgt ccacggccgc
     2701 gtcatccagc caaaccgtca tcgccacggg cgcgccgttg gcgtcctcgt agcgcagcag
     2761 gataaacgcg cgcccctcgg catcgccttt cgccgtgccg ctcaggcgaa aatggccgcg
     2821 aaacatcagg ccttcgaccg gaaaagtcaa aacgacgcgc tgtgccagcc acgacatgcc
     2881 cggaccggag cgcgacaggt ggagcgatcg cgtttgcggg tcgtccgtcg ccgggccgat
     2941 cgccacttcg gccgtctcgc cgcgcgattc aagcgtccag ccattggccc aatcgtcggc
     3001 gaagtcgcca ttctgaataa aattcacttc ggggccaaag gcaatcgttg gcgtgatgat
     3061 gatccccatg atcgtcggca ggggggtgat ggtagcgacg gccgtcggtg gctttgttgc
     3121 ggtcggggtg gcttcggtca acgacgcagt agctgtatgg gccggggatg cagtgggaga
     3181 cggtatggat gtcggcgtat ccgccacaac tgcgggaatg ctcgtggtca tttgcgtggg
     3241 cgcctcggtg atagttcgtc gcaccgggta gatcaaggac aagaatagca ctgcgcaacc
     3301 gaccacgaac agcaataaaa gcaaaaccca cagccaccat cggctcttcc tggccggggg
     3361 cgcactcgtt acctcatcga tcaacggctt gtcgattgcc gcgggctttg tgagtggaga
     3421 ttgaatggat ggaggaacga ccgcttccgg gggcggcgat tccggtttgg ctggagaggg
     3481 cttcccgggc aggttgggcg gcgacgaagg cgcgatgagt tcgcgacaaa ccatgcagat
     3541 ccgcgtaccg ggccggttcg gatgtccgtt cgggcaataa actggttcca tgtgtaaacc
     3601 tggaagccgg gtttcttccg agaaactcgg cttctaacaa tcactggttc gctcaggcag
     3661 tcttgaatcg caggagcaca cgcccaagcc gaatttcatc tccatcttgc aatggctgaa
     3721 tcgcttccgc ggcgagcttc tccttgttga gaaaggtgta gttgaccgag ttgaggtcct
     3781 cgatgaaaaa ttgatcgccg gatcggctta tctgtgcgtg tcgtcgcgag acgccgccct
     3841 cttctccgcc aaaaggcacc agatcgacat cgggatagct gccgctggcg ggatcgatcc
     3901 ggccgatgat atacgtgtcc tggccgggga tcagggagag cacattgccc gccggcatga
     3961 ccagcaggcg ag
//
LOCUS       DAOMQG010000327         3967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9458_length_3967_cov_10.436350, whole genome shotgun sequence.
ACCESSION   DAOMQG010000327 DAOMQG010000000
VERSION     DAOMQG010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_3967_cov_10.436350"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /gene="ettA"
                     /locus_tag="PLC06_13920"
     CDS             complement(<1..1649)
                     /gene="ettA"
                     /locus_tag="PLC06_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRO91590.1"
                     /translation="MTDNKVIYSMVGVGKIVPPNRTILRDIYLSYFYGAKIGVLGLNG
                     SGKSSLLKIMAGVDKEFLGETILSPGYTIGYLEQEPLVDESRTVREVVEEGVQEIVDA
                     LREFDEINARFGEELTPDEMDKLIEDQGKLQDKLDHMDAWNLDSRLDLAMDALRCPPG
                     DTPVSVLSGGERRRVALVRLLLKQPDILLLDEPTNHLDAESVAWLEKHLQQYAGTVIA
                     VTHDRYFLDNVAGWILELDRGHGIPWKGNYSSWLEQKQNRLAKEEKAEGQRMKTLERE
                     LEWIHASPRGRTVKSKARINQYNELLEAAGEKAREELEIYVPPGPRLGDIVIRAEKVD
                     KAFGDRLLVDDMTFNIPPGAIVGVIGPNGAGKTTLFRMIVGQEQPDDGTLTVGQTVKM
                     GYVDQSRETLDPEKTVWQEISGGEETLELGKRKVNSRGYVSRFNFAGADQQKLVGTLS
                     GGERNRVHLAKVLKSGANVLLLDEPTNDLDVNTLRALEEALLTFAGSAVVISHDRWFL
                     DRVANHILAFEGDSQVYWYPGNYSEYEEDKKKRLGAAAERP"
     gene            complement(1739..3535)
                     /gene="metG"
                     /locus_tag="PLC06_13925"
     CDS             complement(1739..3535)
                     /gene="metG"
                     /locus_tag="PLC06_13925"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRO91591.1"
                     /translation="MTEFVLVSIAWPYANSDIHQGNVTGSHLPGDIYARAHRLRGNNV
                     LMVSGSDSHGTPVTVKAEEMGLSNLEVYEHYHARFLDLFRRLGISYDLFTSTHTETHF
                     RVSQDIFTQLLNNGYLYRKVTPQMYSPESGKFLPDRYVEGTCPNCGYTGARGDQCDNC
                     NTLFESAAQLINPRSKRDNTSLVLRDTEHFFLDLPAIADDSLSAWIHDDKEFWRPQVI
                     NFARNFIDQGLIGRAVTRDMDWGIPVPVDDGTFDGKVLYVWFEAVIGYLSASIEWAAN
                     NDKAEAWRNWWQTPDARTLYFIGKDNVPFHTVFWPAQLLGSGGLYSDDPAAHLNLPYD
                     VPANEFMNMEGQKISGSRNWGVWMLDALDRYDPDPLRYYLTANMPETRDSDWSWSGFI
                     ERNNNELVANWGNAVNRVLSMTQRYFGGVVPEPGELTAADTALLAAVDEGFATVAELY
                     NDCKFRAAVQEVLRLSTLVNQYLEETSPWTTVKTDPAAAGRALYVALQAINGLKIMWA
                     PVLPFTSQTLHELLGETGTLFGEQLIRQYDEATRSHLALTYDGTTAVGRWERTPIPVG
                     RHLPKPRPLFRKLEPELAEQEIARLGPKPELQ"
     gene            complement(3539..>3967)
                     /locus_tag="PLC06_13930"
     CDS             complement(3539..>3967)
                     /locus_tag="PLC06_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRO91592.1"
                     /translation="FPGWQERIVMAPTPELAQRIKSGDRFIYYAIEAQTFFAVVTITG
                     EMFEANPKQHTYTTELERAAFFPTDVDATVPALDHGVSVDSIDLESYPDFGKLHIDNE
                     AFYRISEDDFELLAEALTEITEDEDEEVEEYEEYEEDEVE"
BASE COUNT          653 a         1180 c         1302 g          832 t
ORIGIN      
        1 tgcggccgct cggccgccgc gccgagccgc tttttcttgt cctcttcata ttcgctgtag
       61 ttgccgggat accagtagac ctggctgtcg ccttcgaagg ccaggatgtg attggccacg
      121 cggtcgagga accagcgatc gtggctgatg accacggccg acccggcgaa ggtcagcagc
      181 gcttcttcca gcgcacgcaa tgtgttcacg tccaggtcgt tggtcggttc gtcgagcaga
      241 agtacattgg ccccactctt gagcaccttg gccagatgga cgcggttgcg ctcgccgccg
      301 gagagcgtgc cgacgagctt ttgctggtcg gccccggcga agttgaagcg cgacacgtag
      361 ccgcgcgagt tgaccttgcg cttgcccagt tccagtgtct cctcgccgcc gctgatctcc
      421 tgccacaccg tcttttcggg atcgagcgtc tcgcgactct gatcgacgta gcccatcttg
      481 accgtctggc cgacggtcag cgtgccgtcg tcgggctgtt cctggccgac gatcatgcgg
      541 aagagggtcg tcttgcccgc gccgttcggc ccgatgacgc cgacgatagc gcccggcggg
      601 atgttgaagg tcatgtcatc caccagcaag cgatcgccaa acgccttgtc cactttctcg
      661 gcgcggatga cgatgtcgcc caggcgagga cccggcggaa cgtagatctc cagctcttcg
      721 cgggcctttt ctccggccgc ttccagcagc tcgttatatt ggttgatgcg cgccttcgac
      781 ttgaccgtgc ggccgcgggg cgaggcgtgg atccattcca gctcgcgctc caatgtcttc
      841 atgcgctggc cctcggcctt ctcttccttg gccagccggt tctgcttttg ctccagccat
      901 gaggagtagt tgcccttcca gggaatgccg tggccgcggt cgagttccag gatccacccg
      961 gccacgttgt cgaggaagta gcggtcgtgg gtgacggcga tgaccgtgcc ggcgtattgc
     1021 tgcaagtgtt tctccagcca ggcgacggac tcggcgtcga gatggttggt cggctcatcg
     1081 agtagcagga tgtcgggctg cttgagcaat agccgaacga gggccacgcg ccgccgctcg
     1141 ccgccggaga ggacggagac aggcgtgtcg ccgggcgggc agcgcagcgc gtccatggcc
     1201 agatcgagcc ggctgtcgag gttccaggcg tccatgtggt cgagcttgtc ctgcagcttg
     1261 ccctgatctt cgatgagctt gtccatctcg tcgggggtta gctcttcgcc gaagcgggcg
     1321 ttgatctcgt caaactcgcg cagcgcgtcg acgatctcct gcacgccttc ctcgactacc
     1381 tcacgcacgg tgcgcgattc gtcgacgagc ggctcctgct ccaggtaacc aatggtgtag
     1441 ccgggcgaga ggatggtctc gcccaggaat tccttgtcca ccccggccat gatcttgagc
     1501 agggagcttt tgccggagcc gttgaggcca agcacgccga tcttcgcgcc gtagaagtag
     1561 gacagataga tgtcgcgcag aatagtgcgg ttcggtggga cgatcttgcc cacaccaacc
     1621 atggagtaga ttactttgtt gtcggtcata gttaatggtc agtaagtcag taggtcagtg
     1681 ggtcagcagg tcagtaggtc agtattcagt actgacctac tgatccactg aatactgatc
     1741 actgtaattc cggcttcggg ccgagacgag cgatctcctg ctcggccagt tcgggttcga
     1801 gtttcctgaa aagcggccgc ggcttcggca gatggcggcc gacgggaatc ggtgtccgct
     1861 cccagcggcc gacggccgtc gtgccatcgt aggtcaatgc cagatggctg cgcgtcgcct
     1921 cgtcatattg gcgaatcagc tgctcgccga agagcgtccc cgtttcaccc agcagttcgt
     1981 gcagcgtctg actggtgaag ggcaggacag gcgcccacat gattttcagg ccattgatgg
     2041 cctgcaaggc gacgtagagc gcgcggccgg cggcggccgg gtcggtcttg acggttgtcc
     2101 aggggctggt ctcttccagg tactggttga ccagcgtgga gaggcgcagc acttcctgca
     2161 cggcggcgcg gaacttgcag tcgttgtaaa gctcggcgac ggtggcgaag ccttcgtcga
     2221 cggccgccag cagcgcggtg tcggccgcgg tcagctcgcc cggctcgggc accacgccgc
     2281 cgaagtagcg ctgggtcatg ctcagcacgc ggttgaccgc gttgccccag ttggcgacca
     2341 gctcgttatt gttgcgctcg atgaagccgc tccaactcca gtcgctgtcg cgcgtttcgg
     2401 gcatgttggc cgtcaggtag tagcgcagcg ggtcggggtc gtagcggtcg agcgcgtcga
     2461 gcatccatac gccccaattt cggctgccgc tgatcttttg cccttccatg ttcatgaact
     2521 cgttggcggg cacatcgtag ggcagattga ggtgggcggc cgggtcgtcg ctgtagaggc
     2581 cgccggaacc cagcaattgc gccggccaaa acaccgtgtg aaaagggacg ttgtccttgc
     2641 cgatgaagta cagagtgcgc gcgtcggggg tttgccacca gttgcgccat gcctcggcct
     2701 tgtcgttgtt cgccgcccat tcgatgctgg ccgagagata gccgatgacc gcctcgaacc
     2761 agacgtagag caccttgccg tcgaacgtcc cgtcatcgac cggcacgggg atgccccagt
     2821 ccatgtcgcg ggtgacggcg cggccgatga gtccctggtc gatgaagttg cgggcgaagt
     2881 tgatgacctg cggccgccag aactccttgt cgtcgtggat ccacgccgag agcgagtcgt
     2941 cggcgatggc cggcagatcg aggaagaaat gctcggtgtc gcgcagcacc agcgacgtgt
     3001 tgtcgcgctt gctgcgcggg ttgatgagct gggccgcgct ctcgaacagc gtgttgcagt
     3061 tgtcgcactg gtcgccgcgc gcgccggtgt agccgcagtt ggggcaggtg ccttccacgt
     3121 agcgatcagg caggaacttg cccgattcgg gcgagtacat ctgcggcgtc accttgcgat
     3181 agaggtaccc gttgttgagc agttgcgtga agatatcctg cgagacccgg aaatgtgttt
     3241 ccgtgtgggt gctggtgaac aggtcgtagc tgatgcccag gcggcggaac agatcgagga
     3301 agcgagcgtg gtaatgctcg tagacttcca gattgctcag gcccatttcc tcggccttga
     3361 ccgtcaccgg cgtgccgtgg ctgtcggagc cggagaccat cagcacgttg ttgccccgca
     3421 agcggtgggc gcgggcatag atgtcgcccg gcagatggga accggtgaca ttgccctgat
     3481 ggatgtcgga attggcatag ggccaagcaa tgcttaccag cacaaactca gtcataggtt
     3541 attcgacctc gtcttcttcg tactcttcgt actcttcgac ttcctcgtcc tcgtcttcgg
     3601 tgatctccgt caacgcctcg gccagcaact caaaatcgtc ctcgctgatg cgatagaagg
     3661 cttcgttgtc gatgtggagt ttgccgaaat cgggataact ctccagatcg attgaatcca
     3721 cgctcacgcc gtgatcgagg gcgggcacgg tagcgtccac gtcggtgggg aagaaggccg
     3781 cccgctccag ttcagtcgtg taggtgtgct gcttcgggtt ggcctcgaac atctcgccgg
     3841 tgatggtgac gacggcgaag aacgtctggg cctcgatggc gtaatagatg aagcgatcgc
     3901 cggacttgat gcgctgggcc agttccgggg tgggggccat gacgatgcgc tcctgccagc
     3961 cggggaa
//
LOCUS       DAOMQG010000328         3960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9484_length_3960_cov_4.043022, whole genome shotgun sequence.
ACCESSION   DAOMQG010000328 DAOMQG010000000
VERSION     DAOMQG010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9484_length_3960_cov_4.043022"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLC06_13935"
     CDS             complement(<1..916)
                     /locus_tag="PLC06_13935"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRO91593.1"
                     /translation="MKQDDFDIPEVFRRAMEEAGWRADRDDDDGGRPPRRTVTPSSRP
                     RGVNRMMLLVFLLLLLFFSLGSIADFYTDYLWFGALGFRDLFLKRLGVWVAAFVVAFL
                     VAMAFLAGNWLFARNRALRSTSGGTVTGGTVNGGTVNGASEALAHPRTRTLILVAAAV
                     LAILFALAAGSQWEELLLYLNAEPFGVVDPVFGRDAAFYIFNLPVYRFLQGWLFMLLL
                     ITLAGLPMIYTTINLGELQRGRWRPLAAPLLRAHAAALGALLLFVWSVDYFFDIAGLL
                     YSSRGVAYGASYTDMSVVIWALRLQIILA"
     gene            complement(966..3227)
                     /locus_tag="PLC06_13940"
     CDS             complement(966..3227)
                     /locus_tag="PLC06_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HRO91594.1"
                     /translation="METHWLIRLSTRLRPRYGWLPFFLLAGALAGLLFSVQQVGWVRD
                     DGFIIPVLTTGFISAFILAQSKIRPWAAGAILIVMGLALALVLVADLRPDGALLAHGP
                     AAVAEFARLRAALFLGRMAGWFRAVASGGRSSETAAFALGLALAGWLVAAFLPWSAYR
                     MRRPFLGLTLVGLALAANTFYGRAGLYWVALFFGAAITGGVYLAHLFREETWEARGID
                     YPADVRTDLLFYTAGLSIGVMSLAAVLPAVNLRAIAEPFQNWRVVTAAEEMLDRAFGG
                     VAISGRPAGGSEGLPRSFLLGGSPDLAATTVMTATYRAEPAADLARFHWRYTSYDEYT
                     GDGWRRSPERNEAFAGGQPITPPMAPPVAGTLVRITQQVDWLQDQRITRYTLGRPAVF
                     SHDVMTAWRGQDDFVGALGQNDPPARYAAESTVVVATPDELRAARPEDVPPTIRARYT
                     GLPDSIPRRVLDLARRIAAPEGSSLSPYDQARAIEAFLHQYPYTLDLPPPPADVDIVD
                     YFLFDAQTGFCDYYASAMVVMARAVGLPARLGVGFMQRPPDAAGVQTIRLLDAHSWAE
                     VYFAGVGWVEFEPTAPFAVVGAAAAPGVTGDAPAAPTPPSPSPAIPQRAPRREFPWAM
                     VLAVAALVLVALRLQGGRIPALFAPRYADLDEVESAYARLQAGAARLGVVVEPSQTPA
                     EFAEAMAAASAFDMSEVRPLRQAIERLAGLFAARRYGAMDDGASDEAAEAEARRIWSE
                     LDAPLRRLIRRRR"
     gene            complement(3236..>3960)
                     /locus_tag="PLC06_13945"
     CDS             complement(3236..>3960)
                     /locus_tag="PLC06_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRO91595.1"
                     /translation="RSPLATINAATGREYQTHDPYRRIHWPTTARRGSLYVRQFERDA
                     AGDVRLVIDCHAAVQLGEGADGTEEQAVLLAASLTARALGETRSAGLNAYGREPQIVT
                     PGLGEGQQWRVLRALALLRADGEIDLNRALRELGRSARRGSAAVIITPTADAGWLPQL
                     AQLTRQGVECRVVLLDRASFGGEGHSEALRQAINRLGIRCAVVRRGELGRPVTTEAHH
                     GYWEFRVTGTGKAVAVRRPEDA"
BASE COUNT          702 a         1360 c         1389 g          509 t
ORIGIN      
        1 cggcgagaat gatctgcaag cgcaaggccc agatgaccac gctcatgtcg gtgtaactcg
       61 cgccataggc cacgccgcgc gacgagtaga gcagcccggc gatatcgaag aagtagtcca
      121 ccgaccagac gaacaggaga agcgccccca gcgcggcggc atgggcgcgc agaagcgggg
      181 cggcgagcgg ccgccatcga ccgcgctgca gttcgcccag gttgatcgtc gtgtagatca
      241 tcggcaggcc ggccagcgtg atgagcagca gcatgaagag ccagccctgg aggaaacggt
      301 agaccggcaa attgaagata tagaaggcgg cgtcgcggcc gaagacggga tcgaccacgc
      361 cgaacggctc cgcgttcaga tagagcagca gctcttccca ttggctgccc gcggccagcg
      421 cgaacaggat cgccagaact gcggcagcga cgagaatgag ggttcgcgtc cgggggtggg
      481 ccagagcctc gctcgcgccg ttgacggttc caccgttgac ggtgccaccg gtaacggtgc
      541 caccactggt ggaccgcagc gcgcggttgc gggcgaacag ccagttgccc gccaggaacg
      601 ccatggcgac gaggaaagcg acgacgaaag ccgccaccca cacgcccagc cgcttcagga
      661 acagatcgcg gaagccgagc gcgccgaacc acaggtagtc ggtatagaaa tcggcgatgg
      721 agccgaggct gaagaagagc agcagcagca gaaagaccag cagcatcatc cggttgacgc
      781 cgcgcggccg cgacgagggg gtgacggtgc ggcgcggggg gcggccgcca tcatcatcat
      841 cgcggtcggc gcgccagccg gcttcttcca tggcccggcg aaaaacttcg gggatatcga
      901 aatcgtcctg tttcatgggt acgtctattc attgtagccc caaacggggc atcttcgcca
      961 catcttcacc gtcgccgtcg aatgagccgg cgcaggggcg cgtcaagctc gctccagatg
     1021 cggcgggctt ccgcttcggc cgcctcgtcg gatgccccat cgtccatcgc gccataccgc
     1081 cgggcggcga acagcccggc cagccgttcg atggcctgcc gcagcggccg gacttcggac
     1141 atatcgaacg ctgacgcggc cgccatcgcc tccgcgaact ccgccggcgt ttgcgacggc
     1201 tcgacgacga cgcccagccg ggccgcgccc gcctgcaagc gggcatacgc gctctctacc
     1261 tcatcgagat ccgcgtagcg cggcgcgaac agggccggaa tacgcccgcc ctggagccgg
     1321 agcgcgacca gaaccagcgc agcgacggcc aacaccatcg cccaggggaa ctcacgccga
     1381 ggggcgcgtt gggggatggc cggtgagggg gacgggggcg tgggcgcggc cggcgcgtcg
     1441 cctgtcaccc ccggagcggc cgcggccccc acgacggcaa acggcgcggt cggctcgaac
     1501 tccacccagc cgacgccggc gaaatagacc tcggcccagg agtgggcgtc cagcaggcga
     1561 atggtctgca cgccggcggc gtcgggcggc cgctgcatga acccgacgcc cagccgcgcc
     1621 ggcaacccca cggcgcgagc catgaccacc atcgccgagg cgtagtaatc gcaaaagccc
     1681 gtctgcgcgt caaagaggaa ataatcgacg atatcgacgt ccgcgggcgg gggtggcagg
     1741 tcgagcgtgt aggggtactg gtgcaggaag gcctcgatgg ctcgcgcctg atcgtagggc
     1801 gacagagagc tgccctcggg cgcggcaatc cggcgcgcca gatcgagaac acgccggggg
     1861 atgctgtcgg gcaagcccgt gtagcgcgcg cgaatcgtgg gcggcacgtc ttccggccgg
     1921 gcggcgcgca gctcgtcggg cgtggccacg accactgtcg attcggccgc atagcgtgcc
     1981 ggcgggtcgt tttggccgag cgcgccgacg aaatcatcct gcccgcgcca ggctgtcatg
     2041 acatcgtggc tgaaaaccgc cgggcggccg agcgtgtagc gcgtgatccg ctggtcctgg
     2101 agccaatcga cctgctgcgt gatgcgcacc aatgtccccg cgacgggcgg cgccattggc
     2161 ggcgtgatgg gttggccgcc ggcaaacgct tcgtttcgct ccggggagcg ccgccagccg
     2221 tcgccggtgt actcgtcata gctggtgtag cgccaatgga agcgggcgag atcggccgcg
     2281 ggttcggcgc gataggtggc cgtcattacg gtcgtggcgg cgaggtcggg gctgccgcca
     2341 agcaggaatg agcgcggcag tccctcgctc ccgcccgccg gccggccgga gatagccacc
     2401 ccgccaaagg cgcgatccag catctcctcg gcggccgtga cgacccgcca gttctggaac
     2461 ggctcggcga tggcccgcag attgacggcc ggcaggacgg ccgccagcga catgacgccg
     2521 atgctgagac cggcggtgta gaagaggagg tccgtgcgca cgtcggccgg gtagtcgatc
     2581 ccccgtgctt cccatgtctc ttcgcgaaac agatgggcca ggtaaacgcc gccggtgatc
     2641 gccgctccga agaagagcgc cacccaatac aaacccgccc ggccgtagaa tgtgttggcc
     2701 gccagggcaa gcccgacgag cgtgaggccc aggaaggggc gacgcatccg gtaggcgctc
     2761 cacggcagga aggcggccac cagccagccg gccagcgcca aacccagggc gaaggcggcc
     2821 gtctcgctgc tgcggccgcc actcgccacc gcccggaacc agcccgccat gcgaccgagg
     2881 aagagagccg cccgcaggcg ggcaaattcg gcgacggccg ccggcccgtg cgcgagcaac
     2941 gcgccgtccg gccggagatc ggcgacgaga accagcgcca gcgccagtcc catgacgatg
     3001 agaatcgccc cggccgccca cggcctgatt ttgctctgcg ccaggatgaa ggcgctgatg
     3061 aatcctgtcg tcaggacagg gatgatgaag ccgtcgtcgc gaacccagcc cacctgctgc
     3121 accgagaaca gcaggccggc cagcgccccc gccaacagga agaacggcag ccagccgtag
     3181 cggggtcgga gtcgggttga gaggcggatg agccaatggg tttccatggt tgcgctcagg
     3241 catcctcggg gcgacgtacg gccaccgcct tgcccgtgcc ggtgacgcga aactcccaat
     3301 agccgtgatg ggcttccgtg gtgaccgggc ggccaagctc gccccggcgc acgaccgcgc
     3361 agcggatacc cagccggttg atcgcctgcc gcaaggcctc gctatggccc tcgccgccga
     3421 atgaggcgcg atcgagcagc acgacgcggc attcgacccc ctgtcgcgtt agttgggcca
     3481 gctgcggcag ccagccggca tcggccgtgg gggtgatgat gacggccgcc gagccacgcc
     3541 gcgccgaacg ccccaactcg cgcagcgccc gattcaggtc gatctcgccg tcggcgcgca
     3601 gcagggccag cgcccgcagg acgcgccatt gttgcccctc gcccagcccc ggcgtcacga
     3661 tttgcggctc gcggccgtag gcgttcaggc cggcgctgcg cgtctcgccc agcgccctgg
     3721 cggttagtga cgccgccagc aataccgcct gctcctccgt cccgtccgcg ccctcgccca
     3781 actggacggc ggcgtgacaa tcgatcacca gccggacgtc cccggcggca tcccgctcaa
     3841 actggcgcac gtagagggaa ccgcggcggg cggtcgtcgg ccagtggatc cggcgatacg
     3901 gatcgtgagt ctggtattcg cgccctgtgg ccgcgttgat cgtggcgagc ggcgagcgtt
//
LOCUS       DAOMQG010000329         3956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9492_length_3956_cov_6.012817, whole genome shotgun sequence.
ACCESSION   DAOMQG010000329 DAOMQG010000000
VERSION     DAOMQG010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_3956_cov_6.012817"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1216)
                     /locus_tag="PLC06_13950"
     CDS             complement(44..1216)
                     /locus_tag="PLC06_13950"
                     /inference="COORDINATES: protein motif:HMM:NF021599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HRO91596.1"
                     /translation="MLSTLRHQLEHHFREQQRFAEGYSPLYARLFAIVADWLAGDAAD
                     PVVDWLLAAAAERPPFDVTLLLMAALHRDVLAHEPAMAELGRYFMTDVVGLPSADTLR
                     AAVLARRDALGEFIRTANVQTNETGRGLAWLLPAACFGWPAVHLVELGASAGLNLVAD
                     RRVYRLADAADPSQTLLQLGAGSPQFTTLMRDDGALPASVTVPTILSRTGGDVRPFHL
                     RTPDDELTLASFIWGDQPQRLARLREGVAALRAVEQSADGSPLVRLAALNLPDELPRF
                     LAEQLPRPLDAPVVLYNTVVTLYLPDHGASLRGMVAEWAERLSVPVLWLQWEPWWGGP
                     PPPGKEWMAWTADYWPNDGQGDSCRPAARYHLAWVHPHGAALEWAAGWPEFLRKTM"
     gene            1356..2456
                     /locus_tag="PLC06_13955"
     CDS             1356..2456
                     /locus_tag="PLC06_13955"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HRO91597.1"
                     /translation="MNKIQTGTLLIADITGYTLFLNESELAHAQEILTTLLNILIKQT
                     RPPLIISRTAGDAVISYSLGGTHIQGQTFIELLEDTYIAFRRAIELMVLNNRCQCAAC
                     ANVGALDLKFFVHNGEFAIQKLGDHDELVGNEVNQLHRLLKNHIVDALGIRAYTVYTA
                     AAVDALGLGEIAAAMTPHVEEYEHLGEVKVFVQDMKPIWEARRDSTFVDIPPKDILFS
                     VSRDFPLPPHLLWNALTEPETRNLLMGSVSTGVDGRKEGRLAEGTTLHCNHGDSTTEQ
                     LILTWRPFEMMLTEDDPSMAGVTVLDRLRLMPVDGGTRLEVSFGMGKGNPIARQVVGA
                     MLRMKFKGQIAGRMERLRERLTAQGLETTADR"
     gene            2503..2874
                     /locus_tag="PLC06_13960"
     CDS             2503..2874
                     /locus_tag="PLC06_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO91598.1"
                     /translation="MELGAFSISLSVRNIEASRAFYEKFGFTTMGGDASQNWLILHNG
                     DCVIGLFQGMFEGNILTFTPGWDAAAQPLNSFTDVRELQRQLRAKGVSFVTEADDTTT
                     GPAHFIVVDPDGNSILVDQHV"
     gene            complement(3025..>3956)
                     /locus_tag="PLC06_13965"
     CDS             complement(3025..>3956)
                     /locus_tag="PLC06_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434068.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRO91599.1"
                     /translation="VEAVKLEAACGSAGSAMRQGMLAVASGELDRVLVIGVEKLTEMP
                     HGETTAALATAADADFEVDQGITFVGLNALIMRRYMHEYGYNHQDFAPFAITAHANAA
                     RNPNALFHYAIDEREYQRGRDVAAPISLYDASPIGDGAAALLLVAADSVPGAVRVTAS
                     ASATDTVAVHDREDILWLKAAELSAARAYAQAGMGPEDIDLFELHDAFSIMAALSLEA
                     CGFAERGGGVRLGLDGDIARGGRIPISTMGGLKARGHPVGATGLYQLVEATMQLRGQA
                     GALQVPGARTAMTQNIGGSGATIVTHILTTADR"
BASE COUNT          751 a         1241 c         1285 g          679 t
ORIGIN      
        1 gggattgtag caacatcggg aataaaagta caattgccta gttttacata gttttgcgta
       61 gaaattccgg ccaaccggcg gcccattcca gcgctgcgcc gtggggatgc acccaggcca
      121 gatgatagcg cgcggcgggg cgacaggagt cgccttggcc gtcgttgggc cagtaatcgg
      181 ccgtccacgc catccactcc ttccctggcg gcggagggcc accccaccag ggttcccact
      241 gaagccacag caccggcacc gacagccgtt cggcccactc cgccaccata ccacgcagcg
      301 acgcaccgtg atccggcagg tagagggtga cgacggtgtt gtagaggaca accggcgcgt
      361 cgagcggccg cggcagctgc tcggccagga agcgaggcag ctcgtcgggc aggttcaggg
      421 cggccaggcg gacgagcgga ctcccgtccg ccgactgctc aaccgcccgc agcgcggcga
      481 cgccctcgcg caggcgggcc aaacgctgcg gctgatcgcc ccagatgaac gaggccagcg
      541 tcaactcgtc gtcgggcgtg cgcagatgga atggccgcac gtcgccgccg gtgcgcgaga
      601 ggatagtcgg gacggtcacc gaagcaggga gcgcgccgtc atcacgcatc agcgtggtga
      661 attgtggcga ccccgcgccc aattgcagca gggtttgcga cggatcggcc gcgtcggcga
      721 ggcgatagac gcggcggtcg gcgacgaggt tcagcccggc gctggccccc agttcgacca
      781 aatggacagc gggccaaccg aagcaggccg ccggcaacag ccaggccagc ccccgccccg
      841 tctcgttggt ctgcacgttg gccgtgcgga tgaattcgcc cagggcgtcg cggcgggcga
      901 ggacggcggc gcggagggtg tcggcagacg ggagaccgac gacgtcggtc ataaaatatc
      961 gccccaactc cgccatcgcc ggttcatggg ccagcacgtc gcgatggagc gcggccatga
     1021 gcagcagggt cacatcaaac ggggggcgtt cggccgcggc cgcgagcagc caatcgacca
     1081 cgggatcggc cgcgtcgccc gccagccaat cggcgacaat ggcgaacagc cgcgcataga
     1141 gcggcgaata gccctcggcg aagcgttgtt gctcgcgaaa atgatgttcc agttggtggc
     1201 gaagggttga gagcatcggg gttcgttcgg gtgtcgggcg gggttgctgt ttccacttaa
     1261 tcttattaca tctttcccgg caatacactg ttaagtgccg atctgttata atggggccaa
     1321 cctacatcgc accacgacaa gcaggcagaa atggcatgaa caagattcag accggcacgc
     1381 tgctgatcgc cgacatcacc ggttacacgc tctttctcaa cgaatcggaa ctggcccacg
     1441 cccaggaaat cctcactacg ctgctcaata tcctcatcaa acagactcgg ccgccgctga
     1501 tcatctcgcg cacggccggg gatgcagtca tcagctattc gcttggcgga acgcacatcc
     1561 aggggcaaac gttcattgag cttttggaag atacttatat cgcctttcgc cgagctatcg
     1621 agctgatggt gctcaacaac aggtgccaat gcgccgcctg cgccaacgtc ggcgcgctcg
     1681 acctgaagtt cttcgtccac aatggcgagt tcgccattca gaaattgggc gatcacgacg
     1741 agctggtcgg caatgaggtg aaccagctcc atcggctgct gaaaaaccac atcgtcgatg
     1801 cgttggggat tcgcgcctac acggtctaca cggccgcggc cgtcgacgcc ctggggctgg
     1861 gtgagatagc cgctgccatg acaccccacg tcgaggaata cgagcatctg ggcgaggtga
     1921 aggtgtttgt gcaggacatg aagccgatct gggaggcgcg acgcgactca accttcgtcg
     1981 acattcctcc caaggatatc cttttctcgg tgagcaggga ttttcccctg ccgcctcatt
     2041 tgctatggaa cgcgctgacc gaaccggaga cgcgcaacct cctgatgggg tctgtgagca
     2101 cgggggtcga tggtcgaaaa gagggtcgcc tcgccgaggg cacgacactc cattgcaatc
     2161 atggcgattc aaccaccgag cagctcattc tgacgtggcg gccgttcgaa atgatgttga
     2221 ccgaggatga cccgtccatg gcgggtgtga ccgtcctcga ccggctgcgg ctgatgcctg
     2281 tcgatggggg aacgcgactg gaggtgtcgt tcggcatggg caaaggcaat ccaatcgccc
     2341 gtcaggtggt tggggccatg ctgcgcatga agtttaaggg tcagatcgcc ggccgaatgg
     2401 agcggctgag ggagcgtctg acggcgcagg gtttagagac gaccgctgac cgctgacgga
     2461 cgacggacgc gaccccagtc gcgcgacgac aaaggagcaa atatggaact tggcgcattt
     2521 tcaatcagtt tatcggtcag gaacatcgag gcttcgcgag cattttacga aaagttcggc
     2581 ttcacgacga tgggcgggga cgcttcgcag aactggctaa tcctccacaa cggcgattgc
     2641 gtgattggcc tcttccaggg gatgttcgag ggcaatatcc tgaccttcac gcccggctgg
     2701 gacgcggcag ctcaaccgct caactctttc accgacgtgc gcgagttgca acggcagctg
     2761 cgggcaaaag gcgtctcctt cgtcaccgag gccgacgata cgaccaccgg cccggcccat
     2821 ttcatcgttg ttgatcctga tggcaactcc attttggtgg atcagcacgt ctaaggggga
     2881 gcagttcgaa ggacgaaaga ggcgcgcaca ttattcgtct ccctccgtgg tagacgggca
     2941 agagtcggcg ggcaagagtc cgctcggttg gctgtggtct ttcatcgtgc gataggagtc
     3001 gcagcgctct tcattcatcg cccgtcagcg gtcggcggtc gtgaggatat gagtcacaat
     3061 cgtcgcaccg ctgccgccga tgttctgcgt catggccgtg cgcgcgccgg gaacctgtag
     3121 cgcccccgcc tgcccgcgca gctgcatcgt ggcctcgacc agctgataga ggccggtcgc
     3181 cccgacggga tggccgcgcg ccttcaggcc gcccatggtg gagatgggga tgcggccgcc
     3241 gcgggcgatg tcaccatcca gccccagccg cacgccgccg ccccgctccg caaagccgca
     3301 ggcctccagc gacagggcgg ccatgatgct gaacgcgtcg tggagttcga acagatcgat
     3361 gtcctccggc cccatacctg cctgggcata ggcccgcgcc gccgacagtt cggccgcctt
     3421 cagccacagg atgtcctcgc ggtcgtggac ggccacggta tcggtggccg aagccgacgc
     3481 ggtgacacgc accgcgccgg gaacactatc ggcggccacc aacagcagtg ccgccgcgcc
     3541 gtcgccgata ggcgaagcgt cgtagaggct gatcggcgcg gccacgtcgc ggccgcgttg
     3601 gtattcccgt tcgtcgatgg cgtaatggaa gagcgcgttg gggtttcgcg cggcgttggc
     3661 gtgggcggtg atggcgaatg gcgcgaaatc ctggtggtta tagccgtact cgtgcatata
     3721 gcggcgcatg atgagcgcgt tgagaccgac gaaagtgata ccctgatcca cctcgaaatc
     3781 ggcgtcggcg gccgtggcca gcgcggccgt cgtctcgccg tggggcatct cggtcagctt
     3841 ctccacgccg atgaccagca cccgatccag ctcgccgctc gccacagcca gcatcccctg
     3901 acgcatggcc gatccggccg agccgcaggc cgcttccagc ttcaccgcct cgacgc
//
LOCUS       DAOMQG010000330         3877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9707_length_3877_cov_11.417321, whole genome shotgun sequence.
ACCESSION   DAOMQG010000330 DAOMQG010000000
VERSION     DAOMQG010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9707_length_3877_cov_11.417321"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PLC06_13970"
     CDS             <1..831
                     /locus_tag="PLC06_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRO91600.1"
                     /translation="PGDEVLYLEPVWVSYPPMITLAGGTPVGVTLSADDNFRITADRL
                     RAKITPHTKAIMVNTPNNPTGRVLTREELDAIVEVVLENDLYVISDEIYETIIFDGRK
                     HLSIAAEPGMAERTLTINGLSKSHAMTGWRLGWLVAPPAIAKLAAQMNGQTVSSAANF
                     TMHAAVAALKGPKDTTRAMTDAYQKRRDFMVPALNAIDGVECMDIEGAFYLFPRFPNS
                     TKTSAELAEALIDKAGIASVPGGAFGKSGEGHVRFTIATAMKDLEKAVDKLAKAAHEL
                     "
     gene            complement(917..1717)
                     /locus_tag="PLC06_13975"
     CDS             complement(917..1717)
                     /locus_tag="PLC06_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRO91601.1"
                     /translation="MTENTHTDSLDHAHKHAHKDHVDEIIICRNVHKWYGEFEALKGV
                     SLTVKRGEVVVIIGPSGSGKSTFIRTINRLEEHQEGEIIVDGITLSHDIRNIAEIRRE
                     VGMVFQQFNLFPHLTVLENVMLAPINVRKWTREHAKERAMHWLGRVGIPEQANKYPGQ
                     LSGGQQQRVAIARTLCMEPHILLFDEPTSALDPEMIKEVLDVMRDLALSGYTILAVTH
                     EMGFARTVADRVIFMDSGLIVEENTPDDFFANPKNERTKLFLSQILSH"
     gene            complement(1746..3266)
                     /locus_tag="PLC06_13980"
     CDS             complement(1746..3266)
                     /locus_tag="PLC06_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRO91602.1"
                     /translation="MATHIQNLQTFLRGDTGNFSWRQFIREQLLSSWWNVVWLLLLAY
                     VTYRVVAGQLVAAPVTTGAILLAWLALVGITAVGGALHRHTSLTFWLSKNLFNSITGA
                     LISLVIVLLLLAGIRGFIGWAFVRASFSVNPEVAAQELSQWENPGAKWGAVINNFRNL
                     FVFRFPREQDWRLWLLVLWNVLLFVPSTFVFSREAYRRSRLRRVLTLLWLITPIVAYV
                     LLVGVGSSGPLARLNPDIAWGGFLLTVITAAFGIIASFPLGVMLALGRRSQIRGVPAR
                     LTWLVVIPLAIYLLVTQTIPGLRSAAGLGQQLFSLWPLLLILFAFLFMRYFRGNVVAL
                     LSTVYIEVIRGVPLITVLFMSIILFPIFLPPGMEVLSTWRVLVAVALFAAAYLAENIR
                     GGLQSIPKGQYEAADALGLGTARKYRLIIMPQALRAVIPAIVGQFIGLFKDTTLIETV
                     GLIEFLGVANLISAQQNWLGVRREPYVFIAIVYFVINFMMATYSRRLERRLGVGER"
     gene            complement(3269..>3877)
                     /locus_tag="PLC06_13985"
     CDS             complement(3269..>3877)
                     /locus_tag="PLC06_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRO91603.1"
                     /translation="AKDMARASSAYAAGECDIMAASRAALSAELESISATIPSAIAPV
                     RELPVRFSIPRIEGLNFVGGIKLSPNFAAILIGLTIYTGAFIAEIVRAGIQSVAKGQS
                     EAARALGLSESQRLRLVVLPQALRVIIPPLTSQYLNLTKNSSLAIAVGYPDLWSTAFT
                     TLNQSGRAIQVFLIAMGTYLLFSLTISAFLNWYNRRVALVER"
BASE COUNT          872 a         1189 c         1145 g          671 t
ORIGIN      
        1 cccggcgatg aggtgctcta cctggagccg gtgtgggtgt cctacccgcc gatgatcacc
       61 ctggccggcg gcaccccggt gggcgtcacc ctgtcggccg acgataattt tcgcatcacc
      121 gccgaccggc tgcgcgcgaa gatcactccg cacaccaagg cgatcatggt caatacgccc
      181 aacaacccca ccggccgcgt actgacccgc gaagagctgg acgccatcgt ggaggtcgtc
      241 ctggagaatg acctctacgt catctccgac gagatctacg agacgatcat tttcgacggc
      301 cgcaaacatc tctcaatcgc ggccgagccg ggcatggccg agcgcacgct gaccatcaac
      361 ggcctgtcga aatcccatgc catgaccggt tggcggttgg gctggctggt ggctccgccc
      421 gccattgcca agctggcggc gcagatgaac ggccagactg tctcatcggc ggccaacttc
      481 accatgcacg ccgccgtggc cgccctgaag gggccaaagg acaccacccg ggccatgacc
      541 gacgcttatc aaaagcggcg cgatttcatg gtaccggcgt tgaacgccat cgacggcgtg
      601 gaatgcatgg atatcgaggg ggcgttctat ctgttcccgc gcttccccaa cagcacaaag
      661 accagcgccg aactggccga ggcgcttatc gataaagcgg gcattgcctc agtgcccggc
      721 ggcgcattcg gcaagagcgg cgagggtcac gtgcgcttca ccatcgccac agccatgaaa
      781 gacctggaga aggcggtgga taaactggcc aaagccgccc acgaactata agcattcaac
      841 gcaaagacac ttgagaagaa aaagggatcc gcaatgttct gcggatctct tttttaaatc
      901 cttgcgctaa aggttcttaa tgactcagaa tttgtgacag gaacagcttc gtccgctcgt
      961 tcttcgggtt ggcgaagaag tcatcgggcg tgttctcctc aacgatgagg ccgctgtcca
     1021 tgaagatgac gcggtcggcc accgtgcggg caaatcccat ctcgtgcgtg acggccagaa
     1081 tggtgtagcc cgacagagcc aggtcgcgca tcacgtccag cacctcttta atcatctccg
     1141 ggtcaagggc cgatgtcggc tcgtcgaaaa gcaaaatgtg aggctccatg cacaacgtgc
     1201 gggcgatggc cacacgctgc tgctgcccac ccgaaagctg gccgggatat ttgttggcct
     1261 gctccgggat gccgacgcgg ccgagccaat gcatggcacg ttccttggca tgctcgcggg
     1321 tccacttgcg gacgttgatg ggggcgagca taacattttc cagcacagtc aggtgcggga
     1381 agaggttgaa ctgctggaag accatcccca cttcgcgccg aatctcggcg atgttgcgga
     1441 tgtcatgact cagggtgata ccatcgacga tgatctcgcc ttcctgatgc tcttccaggc
     1501 ggttgatggt gcggataaat gtcgatttgc ccgatcccga cgggccgatg atgacgacga
     1561 cctcgccgcg tttgaccgtc aggctgacgc ccttcaacgc ctcgaactca ccgtaccatt
     1621 tatgcacatt ccggcagatg atgatctcgt ccacgtggtc tttatgggcg tgtttgtggg
     1681 cgtggtccag tgaatcggtg tgtgtatttt ccgtcatgat tccccttaat cggttgggcg
     1741 cgagtctagc gctcgcccac cccgagacgt cgctccaggc ggcggctgta agttgccatc
     1801 atgaaattga tgacgaaata aacgattgca ataaaaacat aaggctcgcg tcgcacgcca
     1861 agccagtttt gttgggccga gatgaggttg gcaacgccca gaaactcgat gaggccaacc
     1921 gtctcgatca acgtcgtgtc cttgaagagt ccgataaact ggccgacaat agccggaatg
     1981 acggctcgca gcgcctgggg catgatgatg agccgatatt tgcgcgccgt acctaatccc
     2041 agcgcgtcgg ccgcctcata ttgcccttta gggatcgact gcaaaccacc gcgaatattc
     2101 tcggccagat aggcggcggc aaagagcgcc acagccacca acacgcgcca cgtgctcagc
     2161 acctccatcc ccggcggcaa aaagatgggg aagaggatga tcgacatgaa caacacggtg
     2221 atgagcggca cgccgcgaat gacctcgata tagaccgtgc tcagcagcgc cacaacatta
     2281 ccccggaagt agcgcataaa aagaaatgcg aagaggatca acagcagcgg ccacagcgaa
     2341 aagagctgtt gccccagccc tgctgcgctg cgcaagccgg gaatcgtctg cgtcaccagc
     2401 aagtagatcg ccaacggaat aacgacaagc caggtgagcc gtgcaggcac gccgcggatc
     2461 tggctgcgac ggccaagcgc cagcatgacc cccagcggga aagaggcgat gatgccgaat
     2521 gcggccgtga tgaccgtcag caggaacccg ccccaggcga tgtcgggatt gaggcgagcc
     2581 agcgggccgc tgctccccac acccaccagc aacacgtagg cgacgatggg cgtgatcagc
     2641 cacagaagcg tcagcacccg gcgcaggcgc gaccggcgat acgcctcgcg gctgaacacg
     2701 aaggtgctcg gaacgaatag caacacattc cacaacacca gcagccacaa ccgccaatcc
     2761 tgctcccgcg gaaagcgaaa cacaaacaag ttgcggaagt tattgatgac cgcgccccat
     2821 ttcgcgccgg ggttctccca ttgcgacagc tcttgcgccg ccacctcggg attgacggaa
     2881 aaactggccc gcacaaatgc ccagccgata aagccccgta tacccgcaag gagcagcaga
     2941 acaatcacca gcgaaatgag cgcgcccgtt atggagttga acagattctt gctcaaccag
     3001 aaggtaagcg acgtgtggcg atgcaatgcg ccgccgaccg ccgtgatgcc gaccagcgcc
     3061 agccaggcca gcaagatcgc cccggtcgtc acaggcgcgg caacgagctg accggccacc
     3121 acgcgatagg tgacgtaggc cagcaacagc agccagacaa cattccacca actgctgagc
     3181 agctgctcgc gaatgaactg ccgccagctg aaattgcccg tgtcgccgcg cagaaatgtt
     3241 tgcaggtttt gaatgtgcgt cgccatcgtt atctctccac cagggctacg cggcggttat
     3301 accagttgag gaaagccgaa atcgtcaggc tgaacaacag gtaggtgccc atcgcgatca
     3361 ggaacacctg gatggctcgc cccgactgat tcaacgtcgt gaacgcagtc gaccacaggt
     3421 cggggtagcc gacggcgatg gccagactgg agttcttggt cagattcaaa tattggctgg
     3481 tcagcggcgg gataatgacc cgcaaggcct ggggcagcac cacaagccgc aagcgctgcg
     3541 actccgacag gccaagcgct cgcgccgcct cgctctgtcc cttggccacg gactggatgc
     3601 ccgcgcgcac gatctcggcg atgaacgcgc cggtatagat ggtcagaccg atgaggatgg
     3661 cggcaaagtt gggcgatagc ttgatgccgc ccacgaagtt caacccttca atgcgcggaa
     3721 tcgaaaatcg gacggggagt tcgcgcacgg gggcgatggc gctcggtatc gtcgcggaga
     3781 tgctttccaa ttcggccgac aatgccgccc ggctcgcggc catgatgtca cattctccgg
     3841 cggcgtaggc agatgaggcg cgcgccatgt ccttggc
//
LOCUS       DAOMQG010000331         3868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9737_length_3868_cov_12.630212, whole genome shotgun sequence.
ACCESSION   DAOMQG010000331 DAOMQG010000000
VERSION     DAOMQG010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9737_length_3868_cov_12.630212"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLC06_13990"
     CDS             complement(<1..410)
                     /locus_tag="PLC06_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRO91604.1"
                     /translation="MDDRSEIVLIGPVGAGKSTIGGILAARLGLPQVSMDDIRTDYYR
                     EIGYDEAYAGQLRAEKGFPALVAYWKPFEAYAVERMLADHRDCVFDLGAGHSVYEDTS
                     LFARVGEALMPFRNVILLLPDPDIETSLSIMESY"
     gene            complement(466..822)
                     /locus_tag="PLC06_13995"
     CDS             complement(466..822)
                     /locus_tag="PLC06_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018931808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HRO91605.1"
                     /translation="MTKPRIYTINFARVYPEYVAKAERKGRTKAEVDTIITWLTGYSQ
                     SGLEAQLEKQSDVETFIAEAPELNPARSQIKGVICGMRVEEIEDPTTREIRCLDKLID
                     ELARGKKMEKILRQAQ"
     gene            complement(915..1211)
                     /locus_tag="PLC06_14000"
     CDS             complement(915..1211)
                     /locus_tag="PLC06_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HRO91606.1"
                     /translation="MSFQAYLDNIEAKTGKTPNEFIELAKEKGYDKPDVKAGVIVDWL
                     KQDYGLGRGHAMALVHVIKNGPEIDDKHVGSEGVHRDESNTLRLDGVANRKPGS"
     gene            1345..2145
                     /gene="dapB"
                     /locus_tag="PLC06_14005"
     CDS             1345..2145
                     /gene="dapB"
                     /locus_tag="PLC06_14005"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRO91607.1"
                     /translation="MTIKVCLAGATGWAGSALARAIAQTEDIELVSAVSRTHAGRILG
                     NVLDEPRLLCPIYGTAEEALDFPCDVFFEYTKPDIARSNVMAALDRGANVVIGTSGLT
                     DNEYAVIGAVAKARERGVLAVGNFALTAVLLMKFAEMAAKLIPQWEIIDYASDRKKDA
                     PSGTVRELAARLARVRPSELTVPLEETQGEAGARGARMGGSQVHAIRLPGYTISAEVI
                     FGMADQTLSIRHDSGTSAEPYVAGALLAIRKMGSFVGLRRGLDSVLDI"
     gene            2213..2449
                     /locus_tag="PLC06_14010"
     CDS             2213..2449
                     /locus_tag="PLC06_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO91608.1"
                     /translation="MNFERITINPKQMGGVPCIRGLRIPVATVISLVAAGMSDDAILE
                     AYPDLEAEDIREALRFAAEAVREQAIPLLIPVAA"
     gene            2446..2817
                     /locus_tag="PLC06_14015"
     CDS             2446..2817
                     /locus_tag="PLC06_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRO91609.1"
                     /translation="MRFLIDNALSPQLAHGLLAAGHDAIHVRDVGLGTAEDETIFDLA
                     LAEERVIISADTDFGTLLAERQESKPSFILFRRIADEPQQQLAILLVNLDSIQEALEN
                     GSVVVIEQHRMRVRLLPITRR"
     gene            2819..3520
                     /locus_tag="PLC06_14020"
     CDS             2819..3520
                     /locus_tag="PLC06_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91610.1"
                     /translation="MDAISPGIGQLTERSLHAALKEHLAQPGDRFEVKLGRYVIDIVR
                     GDLLIEIQTRHLYALRPKLLRLLEDHRVHLVHPLPAERWIVREGRDGRSTRRKSPRHA
                     AARDIFNELVRIPDLAMHPNLTLEALLIREEQLWRDDGQGSWRRGRWSLIDTRLLGVV
                     ESAVFAQPGDYLALLPALPETFTNADLAKAKGWNAHLAGKATYALRRMGRLEEAGKRG
                     RANLFTIEVTHDNNH"
     gene            3504..3758
                     /locus_tag="PLC06_14025"
     CDS             3504..3758
                     /locus_tag="PLC06_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91611.1"
                     /translation="MTITIEFEIEESILAELDGVAADIGVSREILITTAIKHALRFHR
                     PKEFSAEDEVNTFRLWLAGNADVEGGSGIPDWDEDDARLN"
BASE COUNT          780 a         1160 c         1133 g          795 t
ORIGIN      
        1 tcatagctct ccatgatgga cagggaagtt tcaatgtccg ggtcgggcag caacaggatg
       61 acgttcctga atggcattaa ggcttccccg acacgcgcga acaagctcgt atcttcgtaa
      121 accgaatgcc cggctccgag atcgaagacg cagtcgcgat ggtcggccag catgcgctcg
      181 acggcatacg cttcaaatgg tttccaatag gcaaccaaag ccgggaagcc cttctccgcg
      241 cgcagctgcc cggcataggc ctcgtcataa ccgatctcgc gatagtaatc ggtacggatg
      301 tcatccatcg acacttgcgg caagcccagt cgcgcggcca gtatgccgcc gatggtcgac
      361 ttcccggcgc cgacagggcc aatcaggact atttcggatc gatcatccat ggaaagagga
      421 taactgaatt gccggggatg ggaaggcaga ccaacgcaat caaactcact gcgcctgccg
      481 caatatcttc tccatcttct tccccctggc cagctcgtcg atcagcttgt ccagacagcg
      541 aatttctcgc gtggtcgggt cttcgatctc ttccacccgc atgccgcaga tcaccccctt
      601 gatctgtgag cgcgccggat tgagttcagg cgcttcggcg atgaaggtct cgacgtccga
      661 ctgcttctcc agctgcgctt ccagcccgct ctggctatag ccggtcaacc aggtgatgat
      721 cgtatcgacc tcggctttgg tgcgtccctt tctctccgcc ttggcgacgt attcgggata
      781 gactctggcg aagttgatgg tatagattcg gggtttcgtc attgtatttg ttagcctcaa
      841 tggacgtgaa ggggcggccg atgatgcaac tgttacagtt gtcttcgacc accacgccga
      901 tgggggttga acgctcagga gccgggcttg cgatttgcca cgccgtccag cctcagcgtg
      961 ttcgactcat ctcgatgcac gccttcgctc ccgacgtgtt tgtcatcgat ctccgggccg
     1021 ttcttgatga cgtgaaccag ggccatcgcg tggccgcggc ccaggccgta atcctgcttc
     1081 agccaatcga cgatgacgcc ggccttaacg tcgggcttgt cgtagccctt ttccttcgcc
     1141 agttcaatga attcgttggg tgttttgccc gtctttgctt cgatgttgtc gagatatgct
     1201 tgaaatgaca tgatggtatt atagcgcctg ttgccgccgg cgatacaggg cacttccccc
     1261 aatccgctct attctgacaa cttgtgtcgg atggtgtatg atttttatca atctgcaaca
     1321 actccggatg atacatgagg aaccatgacc attaaagttt gtctcgccgg cgcgaccggc
     1381 tgggccgggt cggcgctggc ccgcgccatc gcccaaaccg aagatatcga actcgtctcg
     1441 gccgtctcgc gcacgcacgc cgggcgcatt ctgggcaatg tgctggacga gccgcgactg
     1501 ctatgtccca tctacggcac ggccgaagaa gcgcttgatt ttccctgcga cgttttcttc
     1561 gaatacacca agcccgacat cgcccggagc aacgtcatgg cggcgctgga tcgcggggcc
     1621 aacgtcgtca tcggcacatc gggcctgacc gacaacgagt acgccgtcat cggcgccgtg
     1681 gccaaggcac gcgagcgcgg cgtgctggct gtgggcaatt tcgccctgac ggccgtcctg
     1741 ctgatgaagt tcgccgagat ggccgccaaa ctcatcccgc aatgggagat catcgactac
     1801 gccagcgacc gcaagaagga cgcccccagc ggcaccgtgc gcgaactggc agcgcggctg
     1861 gcccgcgtgc ggccgtcgga actgaccgtg ccgctggaag aaacccaggg cgaggccggg
     1921 gcgcgcggcg cgcgcatggg cggctcgcaa gtccacgcca tccggctgcc cggctacacc
     1981 atctcggccg aggtcatctt cggcatggcc gaccagacgc tgagcatccg ccacgactcc
     2041 ggcaccagcg ccgagccata cgtggccggg gcgctgctgg ccatccgcaa gatggggtcg
     2101 ttcgtcgggc tgcggcgcgg gctggatagc gtgctggata tttaggaatt agaaacgggg
     2161 attcctgagt ggtataatcg ttggaaataa cgctgatttt aggaaaatga ttatgaactt
     2221 cgagcgtatc accatcaatc caaagcaaat gggcggggtt ccttgtatcc gcggcttgcg
     2281 cattcccgtg gccactgtta tcagcctggt tgccgccggt atgtctgacg atgcgatcct
     2341 cgaagcctat cccgatctgg aagcggaaga catccgtgag gcgcttcgct ttgcagccga
     2401 agcagtacgt gaacaagcaa ttccactcct gatcccggtt gcagcgtgag attcctaatc
     2461 gataacgcat tgtcgccgca attggctcat gggttgttgg ctgcgggaca tgatgcaatc
     2521 catgtgcgcg atgtgggcct tgggacggcc gaggacgaaa ccatttttga tctggctctc
     2581 gccgaggaac gagtcattat ttcggccgat accgatttcg gcactcttct cgctgaacgc
     2641 caagaaagca agccttcgtt cattcttttt agacgcattg cagacgagcc gcagcagcaa
     2701 ttggcgatat tgttggtaaa tctggactcc atacaggagg ctcttgaaaa cggcagtgtt
     2761 gtggtgatag agcaacatcg tatgcgtgtt cgtctcttac ccattacaag gcggtaacgt
     2821 ggatgcaatt agtcccggca tcggccaact caccgagcgc tcgctccacg ccgcgctgaa
     2881 agaacacctg gcccagccgg gcgaccggtt cgaggtcaaa ctcggccgct acgtcatcga
     2941 catcgttcgc ggcgacctgc tgatcgagat tcagacccgc cacctctacg ccctgcggcc
     3001 gaagctgctg cgcctgctcg aagatcatcg cgttcacctt gttcacccgc tgcccgccga
     3061 acgctggatc gtccgcgagg ggcgcgacgg ccgcagcacc cgccgcaaat cgccccgcca
     3121 cgccgccgcc cgcgacatct tcaacgagct ggtgcgcatc cccgacctgg ccatgcaccc
     3181 caatctgacg ctggaagcgc tgctcatccg cgaggagcaa ctctggcgcg acgacggaca
     3241 agggagctgg cgtcgcggcc gatggagcct gatcgacacg cggctgctgg gagtggtcga
     3301 gtcggccgtc ttcgcgcaac cgggcgatta tctggccctg ctgccggcgc tgccggaaac
     3361 cttcaccaac gccgacttgg cgaaagcaaa gggctggaac gcgcatttgg ccggcaaggc
     3421 cacgtatgcc ctgcgccgga tggggcggct ggaggaagcg ggcaagcgtg gccgggccaa
     3481 tctgttcaca atcgaggtaa cgcatgacaa taaccattga gtttgagata gaggaatcga
     3541 tattggcgga acttgacgga gttgcggccg acatcggcgt cagccgcgag atacttataa
     3601 cgacagcgat caagcacgcg ctgcgctttc atcgtcctaa agaattcagc gccgaggatg
     3661 aagttaatac gtttcgtctg tggttggccg gcaatgcgga cgtagagggc ggatcgggaa
     3721 taccggattg ggatgaggat gatgcccggt taaattgagc atgaaacgaa gatgaagtaa
     3781 aatccgcaaa ttctgcaccc aggagcaaca ccatgaccga ccaacggccg atcaccatcc
     3841 tctgtctcgc cagcgaattc aagggcgt
//
LOCUS       DAOMQG010000332         3864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9753_length_3864_cov_11.248884, whole genome shotgun sequence.
ACCESSION   DAOMQG010000332 DAOMQG010000000
VERSION     DAOMQG010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9753_length_3864_cov_11.248884"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLC06_14030"
     CDS             <1..795
                     /locus_tag="PLC06_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91612.1"
                     /translation="LTQDATFIEFMREILQHLEIIYQGPVNLEYSLMVGENGESTSNQ
                     RNYQLYILECRPLNERLEPKKKPEPEIDDTKRQLFRMPTLLPSGEVDAIDYLVFVDPE
                     SYYDIEDEDQRNQIAETITALNDILPAGRFGLIGPGRWGSLDSRLSVPITYSDICNTS
                     LLVEISPPYTPAPELAYGTEFFEGVRETGILVLGIQPMPRGGSMDWEFLRHSPNLLTH
                     YVPWATDWADCVRVIDLNAAAGSSLRLVIDEETNEAIACFDEPATR"
     gene            complement(825..3503)
                     /locus_tag="PLC06_14035"
     CDS             complement(825..3503)
                     /locus_tag="PLC06_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO91613.1"
                     /translation="MALILTHEKTDFDAVASQLGAKKLWPAATALLPRHLNRNVLQFL
                     NLHWDSLPFMRADDWRRRPIDSVILVDTQTLSNVRGLASNPQVHVIDHHMGHLPRKGW
                     AYEVEPVGATTTLLVERIQARGIVLTPEEATLMLLGIYEDTGGLSYDTTTARDVRAAA
                     ALLEQGAQLNVARRFLNVALSEQQRHLYDVLIRNVEWVAHEGQEIALTSADVPPGFDD
                     EISSVAHRLRENLMPDGLFVLVQLSDGVQLVARSSVDEVDVGLVARHLGGGGHSRAAA
                     AMIVGGRVSDVARQIREFLPRAIRPALHVASLMSHGVQTVPASMTVAEVAALMQRHGH
                     EGYPVVDGRNGRLVGLITRGAVDRAVNHNMGNQPVSRYMRAGSVFVFPSDSIKRVQQL
                     METEAWGQIPVVAEEGEVPPADRPPIGIVTRTDLLSALFKPPPDLPDTDMRERLARSF
                     SHELWAVVRVVSEMAGRMQMPIYFVGGLVRDLMLNKQPTDIDIVVEGDAIELAEGMSR
                     RFGGEVHSHDRFGTAKWSLGPDVYAAIGQEVKGELAAADIASGSGKRIPAPPQIDFVT
                     ARKEFYKRPTALPDVEPGSIKLDLHRRDFTINTLAVRLDGDYLGQLLDFYGGRRDLRR
                     GIIRVLHSLSFIDDPTRILRAVRLEQRLGFTIEKNTAELIEDALPLLDRVSGERIRNE
                     IELSLKEIDPVKVMERLDGLGVMAQLHPQLCWQPETVTVFRRIPVYSDNPLWREIYHS
                     GATVFFYFAAWLAPFGRTVREAVAGRLRVRKSTLDDLLALDTLQSGLAALPPDARPSR
                     IVAVLEPLASRVLLMARMLDPGEPINGWLERYVAEWRHVRTTITGDDLRAAGLPPGPA
                     YTRILGKLLAARLDGEIRGDAEERALYESLVAAETA"
     gene            3668..>3864
                     /locus_tag="PLC06_14040"
     CDS             3668..>3864
                     /locus_tag="PLC06_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91614.1"
                     /translation="MVNKNSFQFGAVNSQDILSRKMIITAFLAVALIAFEIFNFDTTK
                     FALHNLLGDVKFAGVLWASILA"
BASE COUNT          895 a         1173 c         1060 g          736 t
ORIGIN      
        1 ctgacccagg atgcgacttt catcgaattc atgcgcgaaa tattgcaaca tctggagatc
       61 atctatcaag gcccggtcaa tctggaatat tcgcttatgg tgggagagaa tggggaatca
      121 acaagcaacc agcggaacta ccaactctac atcctggagt gccggccgct caacgaacgt
      181 ctggagccga agaaaaagcc cgaacccgaa attgacgaca ccaagcgcca actcttccgc
      241 atgccgaccc tgttgccttc aggggaggtt gacgccatcg actatctcgt attcgtcgat
      301 ccggaatcat attacgatat cgaggatgaa gaccagcgca accaaatcgc cgaaacgatc
      361 accgcactca acgacatact gccggccggc cgttttggtc taatcgggcc gggtcgttgg
      421 ggttcgctgg acagtcgtct cagtgtgccg atcacctata gcgatatctg taacaccagc
      481 ctgttggtcg agatcagccc gccctacacc cccgctcccg aactggccta tgggacagag
      541 ttcttcgaag gcgttcggga aacagggatt ctcgttttgg gaatccagcc gatgccgcgc
      601 ggagggtcga tggattggga atttctgcgc cactcgccca accttctgac gcactacgtc
      661 ccctgggcga ccgactgggc ggattgcgtg cgggtcatcg atctgaatgc ggccgccggc
      721 tcatccctga ggctcgtcat cgatgaagag accaacgagg ccatcgcctg ctttgatgaa
      781 cctgcgaccc gctaagaaga aaaatggggc aatcgtgtga aaggctaggc ggtttcggcc
      841 gctacgagcg actcatacag cgctcgctct tcagcgtcac cgcggatctc gccatcaagc
      901 cgcgccgcca ggagtttgcc aagaatacgc gtatacgcag ggccgggtgg caatccggcc
      961 gcccgcaaat catcgccggt gatcgtcgtt cgcacatgcc gccactcggc cacatatcgc
     1021 tccagccagc cattgatcgg ctcgcccgga tcaagcatgc gggccattaa caagacgcgc
     1081 gacgctaacg gctcgaggac agctacaatc cggctcggcc gcgcgtccgg cggcaacgca
     1141 gccagaccgg actgcaacgt atccaaagcg aggagatcat ccagggtgga ttttctcacc
     1201 cgcaatcgcc cggccaccgc ctcgcgaaca gtccgaccaa acggagccag ccacgcggcg
     1261 aaataaaaga aaaccgtcgc gccggaatga tagatctcgc gccatagggg attgtcgctg
     1321 taaacgggaa tgcgcctgaa aacagtgacg gtttcgggct gccagcataa ttgcgggtgg
     1381 agttgggcca ttacccccaa cccatcgaga cgctccataa ccttgaccgg atctatctcc
     1441 ttcagactca gctcaatctc gttgcggatt cgttcgccgc tgacccggtc gagtagcggc
     1501 agggcatcct cgattagctc ggccgtgttc ttctcaatgg taaaccccaa cctttgctcc
     1561 aatcggacag cgcgcaaaat acgcgtcgga tcatcgatga agctcaaact atggagcacg
     1621 cgaataatgc cgcgacgcaa atcacgccgc ccaccgtaga aatccagcaa ctggccaagg
     1681 taatcaccat ccagacgaac ggccagcgtg ttgatggtga aatcacgccg gtggaggtcc
     1741 agcttaatcg aacccggttc cacgtcgggg agggctgtcg gccgcttgta aaactccttg
     1801 cgcgcggtca cgaagtcgat ctgcggcggc gcggggatgc gtttgccact gccggaagct
     1861 atatccgcgg ccgcaagctc acccttcacc tcctgcccga ttgcagcgta aacatccggc
     1921 cccagcgacc atttggcggt cccgaatcga tcgtggctgt gtacctcgcc accgaagcgc
     1981 cggctcatcc cctcagccag ttcgatggcg tcgccttcta caacgatatc gatgtcggtc
     2041 ggttgtttgt tgagcatcag atcgcgcacc aggccgccga cgaagtagat aggcatttgc
     2101 atccgcccgg ccatctcgct aaccactcgc accactgccc ataattcatg cgaaaaagaa
     2161 cgggccagtc gctcgcgcat gtcggtgtca ggcaggtcgg gcggtggctt gaacaacgcg
     2221 cttagcagat cagtgcgagt tacgatgccg atgggcggcc gatccgcggg aggaacctcg
     2281 ccttcctcgg caacgacggg gatttgcccc catgcttcgg tctccatcaa ctgctgaaca
     2341 cgcttgatcg agtcggaagg aaacacaaag acggatccgg cgcgcatata gcgactaacc
     2401 ggctgattgc ccatgttgtg gttaacggct cgatccacag caccacgagt gatcaggcca
     2461 acgaggcggc cgtttcgccc atccacgacc ggatagcctt cgtggccatg acgctgcatc
     2521 agcgccgcca cttcggcgac agtcatcgaa gccggaaccg tctgcacacc gtgggacatg
     2581 agcgaagcga catgcagcgc cggccggatc gcgcgcggca agaactcgcg aatttgtcgg
     2641 gcaacatccg acacccgtcc gccgacgatc atggccgccg cggcgcggct atggccgcca
     2701 cctcccaaat gacgggcgac caggccgaca tcgacctcat cgactgaact acgggcgacc
     2761 aactgcacgc catcactcag ttggaccaga acgaacagcc catcgggcat gagattttct
     2821 cgcaggcgat gggcaaccga ggaaatttcg tcgtcaaagc caggtggaac atcggccgag
     2881 gtcagagcaa tttcctggcc ttcatgcgcc acccattcga cgttgcggat aagaacatca
     2941 tacagatgac gttgctgctc ggacaaggcg acgttgagga aacgacgcgc aacatttaac
     3001 tgtgccccct gctccagcaa ggcggcggcc gcccggacgt cacgggcagt tgtcgtatca
     3061 tagctcagcc cgccggtgtc ctcgtagatg cccagcaaca tgagcgtcgc ttcttcaggg
     3121 gtgaggacga taccccgtgc ctggatgcgc tccacgagca gcgtcgtcgt ggcaccaacc
     3181 ggctccactt cataggccca tcccttcctg ggcagatgtc ccatgtgatg atcgatcaca
     3241 tggacctgcg gattggatgc caacccgcga acattactca aggtctgcgt atccaccagg
     3301 atcacggaat cgatcggccg ccggcgccag tcgtcggctc gcataaacgg caatgaatcc
     3361 caatgcagat tgaggaattg cagcacatta cggttgaggt ggcgcggcag cagagcggtt
     3421 gccgccggcc aaagcttttt ggctcccaat tgcgaggcaa ccgcatcgaa atccgtcttt
     3481 tcgtgggtca ggatcaacgc cataaaggaa ttatacacga accacagtag ccacagaatg
     3541 cggccatata tgccgtacta tgcgtattga cgaaaacaga acaatagtgc tattattcac
     3601 ccataaagaa caactgttct aattccttac agacggaaga gttgggttga ataaggagaa
     3661 atgcgacatg gttaataaaa acagttttca attcggggca gtgaacagcc aggatatcct
     3721 atcaaggaag atgatcatca ccgcatttct tgccgtggcg ctcatcgcct ttgaaatctt
     3781 caactttgac acgacaaaat tcgcgctcca caacttgctg ggggatgtca agtttgccgg
     3841 agtcttatgg gcgtccatat tggc
//
LOCUS       DAOMQG010000333         3831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9857_length_3831_cov_10.931409, whole genome shotgun sequence.
ACCESSION   DAOMQG010000333 DAOMQG010000000
VERSION     DAOMQG010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9857_length_3831_cov_10.931409"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLC06_14045"
     CDS             complement(<1..344)
                     /locus_tag="PLC06_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRO91615.1"
                     /translation="MTQIIDEDAAAADDGGMMSILDHLNELRRRLTFAAIAILITTVF
                     SFIFAEQLLAFLLAPYSRSIEGGAALQTLRPTEGIETYFRVSLLSGIILAMPVILFEF
                     WRFIRPALRKNE"
     gene            complement(341..880)
                     /locus_tag="PLC06_14050"
     CDS             complement(341..880)
                     /locus_tag="PLC06_14050"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRO91616.1"
                     /translation="MDSFFGIGLPEFVLILVIAGMVMGPERIVRMARSLGVVTARLQN
                     ISRSFLRQLSAELDSVDDTGALRSTAEELKQLKQQVADLRDEVLTLAGEGIAPPKGSK
                     VTNAGSSNSIMPRSLPTVSSPNGPGKASSDHKAAGEGSPPPTSKVPAKSSNDSAAVGR
                     VPHPHQLPRRVDIPEDPDA"
     gene            complement(890..1087)
                     /locus_tag="PLC06_14055"
     CDS             complement(890..1087)
                     /locus_tag="PLC06_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRO91617.1"
                     /translation="MGSIGLPELLVVLAVVLLVFGVGRIARVGGELGKGVSAFREGLR
                     EGQEAPSEKDSTEKKDDTPAA"
     gene            complement(1175..2848)
                     /locus_tag="PLC06_14060"
     CDS             complement(1175..2848)
                     /locus_tag="PLC06_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRO91618.1"
                     /translation="MKPDIRWQLLLASTGLLLVLMLLSYQVQSAALCTVTVPATGGTY
                     IEGMVGRPNSLNPLLSDLYPVDREINNLIFDGLVRYDEKGQLKPALAKEWSVSEDGLT
                     FTFILEDGRTWHDGQPVTTADVAFTYGLMQKEDFPGPPSLISLWQSVTITPIDEQQIA
                     FTLSQPYAPFFEATTRGILPAHLLEDVPVSDLATHPFNQAPIGTGPFMVQAGQDWTRT
                     GRLRLSPNPASWRQGVQISDIEIRFFADADSLITAFRNGEVHAVNGLSPTAIPQLAAE
                     TSARIFTSIAPRYSALFFNLSESGAPALQSREVRQAIGFALDREALINSILNGQGIRF
                     EGPYLPDNWAARLDLMTTYNYQPETAGSLLDSIGWVGAGTRIREGVPLVLKFVVLDTP
                     EQRAISEEIANQLSRIGIEAQITLVADAASLQETMAERQFDMALVEVAPPNDPDLYDF
                     WSQEAIVRGHNYSGWNNRRASEALENGRQILPQGERAAYYEAFLRQFDADLPALTLYQ
                     HVDTYMLSDVVKGVEIGRVWEPRDRYQSLPAWFLNYRDVAVSCPSGEAN"
     gene            complement(2881..3135)
                     /gene="secG"
                     /locus_tag="PLC06_14065"
     CDS             complement(2881..3135)
                     /gene="secG"
                     /locus_tag="PLC06_14065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRO91619.1"
                     /translation="MNLQPWAPYLSIIVIILGIALSALVLLQSKGQDLGGFLGGGGGD
                     GGTFRTRRGVEVVLHRLTIIIAVLFFVFTLLAFLAWGQVS"
     gene            3335..>3831
                     /locus_tag="PLC06_14070"
     CDS             3335..>3831
                     /locus_tag="PLC06_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91620.1"
                     /translation="MIEFNQVSKKYYSLTALRDLNLTIPQGEVIGLLGPNGAGKTTFI
                     KLIAGIIMPTKGEIRATDSEAGWPSIGYKSERLVFPDNLVASQYLEMIAGLCSIKRAQ
                     RDNVVLESLARVNLLAAANKQIKSLSKGMRQRLGLAQAIIGDPDLVLLDEPSSGLDPE
                     GQDEM"
BASE COUNT          944 a         1053 c         1020 g          814 t
ORIGIN      
        1 tcttcgtttt ttcgaagcgc cgggcgaata aagcgccaga actcgaacag gatgaccggc
       61 atggccagga tgattcccga caaaagggaa acccgaaaat aggtttcaat accttccgtc
      121 ggccgcaggg tttgcaacgc agcgccccct tcaatacttc gggaataagg tgccagcaga
      181 aacgcgagga gctgctctgc gaagataaac gaaaatacag tcgtgatgag aatcgcgata
      241 gccgcaaacg tcaaacgccg ccgtagctca ttaaggtgat ccaggatgct catcatcccg
      301 ccatcgtccg ctgcggccgc atcctcatcg ataatctggg tcatgcgtcc gggtcctcag
      361 ggatatcgac gcgcctgggc aattgatggg ggtggggcac gcgacctaca gcagcgctat
      421 cattgcttga tttcgcaggc acttttgagg ttggcggcgg cgaaccttcg ccggcggctt
      481 tatgatccga ggatgccttg cccgggccgt ttggagagga tactgtgggc aaagagcgag
      541 gcattattga attgctcgac cctgcattgg ttacttttga acccttcgga ggcgcgatgc
      601 cttctccagc cagggtgaga acttcatcgc gcagatcggc cacctgttgc ttcaattgtt
      661 tcagttcctc ggccgtgctt cgcaatgcgc ctgtatcatc cacagagtcg agctcagcac
      721 tgagttgccg aagaaaagac cgcgagatat tttgcaggcg cgcagtaaca acgcctaatg
      781 agcgggccat gcgaacgatt cgttccggcc ccatgaccat tccggctatt accagaataa
      841 ggacaaattc cggtaggccg atgccgaaaa agctgtccat ccggcaaatt taagctgcgg
      901 gggtgtcgtc tttcttctcg gttgaatcct tctcggacgg ggcctcttgc ccctctctta
      961 gtccctcgcg gaacgccgac acgcccttgc caagctctcc gccgacacga gcgattcggc
     1021 ctacgccaaa gaccaacaat acgacggcca agaccaccag cagttcgggt aaaccaatcg
     1081 atcccatcag attctcctta caccggcatg aacgagccgt gcatagacaa ttataacaaa
     1141 gaaccggcgc aatatcggtt ccttcccatt actctcagtt ggcctccccc gaagggcagc
     1201 ttacagctac gtcgcgataa ttgaggaacc aggccggcaa tgactgatat cgatcgcgtg
     1261 gttcccagac gcggccgatt tccaccccct tgactacgtc acttagcata taagtatcga
     1321 cgtgctgata gagagttagg gcgggtaaat cggcatcaaa ttgacgcagg aatgcctcat
     1381 aataggctgc ccgttctccc tgtggaagga tctgccgacc attctcaagc gcttcggacg
     1441 cccgacgatt gttccagcca ctgtaattat ggccgcgaac gatggcttcc tgactccaga
     1501 agtcgtagag gtcgggatca ttcggcgggg cgacctcgac caacgccata tcaaactgcc
     1561 gctcggccat ggtctcctgc aacgaagcag catcggccac gagcgttatc tgggcttcaa
     1621 ttccgattct cgacaattga ttggcgattt cctcgcttat agctcgttgc tctggtgtgt
     1681 cgagaacgac gaacttcaac accaggggta cgccttctct tatccgagtc cccgctccca
     1741 cccaacctat tgaatcaagc aggctgccgg ctgtttccgg ctggtaatta taggtagtca
     1801 tcaggtccag acgagctgcc caattatccg gtaagtaggg accctcaaaa cgaatcccct
     1861 gaccattgag aattgaattg ataagcgctt cacggtcaag cgcgaagcca atcgcctgcc
     1921 tgacctctct ggattgaagc gccggtgcgc cggattcact caaattaaaa aagagggccg
     1981 aatagcgtgg agcaattgag gtgaaaattc gggcagatgt ctcggccgcc agctgcggaa
     2041 tggccgttgg tgacagtcca ttgaccgcat gaacctcacc attgcgaaag gcagtgatca
     2101 ggctatcagc atcagcaaaa aatcgaattt caatatcgct tatctggacg ccttgccgcc
     2161 aggaagccgg gtttggggac agcctcaaac ggcctgtccg agtccaatcc tgacctgcct
     2221 gaaccataaa tgggcctgtg ccaataggcg cttgattgaa aggatgcgtt gccaggtctg
     2281 acacagggac atcctctagc aagtgcgccg gcaagatacc ccgcgtagtc gcctcaaaga
     2341 atggggcata aggctgggat agggtaaaag cgatctgttg ttcgtcaatc ggcgtgatgg
     2401 tgacggattg ccagagagaa atgaggctcg gcgggccggg gaaatcctct ttttgcatca
     2461 atccgtaggt aaacgcgaca tcggccgttg tgaccggttg gccgtcatgc caggtccgcc
     2521 catcctcaag gataaacgtg aacgttaagc catcctcgct cactgaccat tctttggcca
     2581 acgccggttt caattggcct ttttcatcat agcgaacgag accgtcgaaa atcaggttat
     2641 tgatttcccg atcaacaggg tagagatcgc tcaggagggg gttgagagaa ttgggccggc
     2701 caaccatgcc ctcaatatat gttccgcccg tggccggcac ggtaaccgtg cacaaagcgg
     2761 cggattgcac ctgataagac aaaagcatca acaccaggag caatcccgtg gacgccagca
     2821 gaagctgcca gcgaatatcg ggtttcatgc gaagatgttt accgtttggg ccggccgctc
     2881 ctaactaact tgcccccacg ccaggaaagc caggagcgtg aagacgaaga acaggacagc
     2941 gataataatc gtcagccgat gaagcacgac ttcgacgcca cgtcgcgtac ggaaggtgcc
     3001 accatcacca ccgccaccgc cgagaaaacc gcccaggtcc tgtcccttcg attgcaacaa
     3061 gaccagtgcc gacagggcaa tgcccagaat aatgacaatg atgctcagat atggcgccca
     3121 aggttgtaag ttcataacag tgaatatcct ctcataaccg gcaatgccgg gaagtttcca
     3181 acgaacgcga aattataccc ggaagggggt ggcccgtcga ggggcggttg cggcagctta
     3241 acaaatataa atggaattgc tatggatgat gggtgtgata aactcgtccg gtatcgcatc
     3301 ttttccggca aatcacagcc cttcggacct acacgtgatc gaattcaacc aggtatcaaa
     3361 aaagtattat tcccttactg ccttgcggga tctcaatctg acaattcctc agggagaagt
     3421 aattggtctg ttaggtccaa atggtgcagg gaagacgacc tttatcaagc tcattgccgg
     3481 aatcatcatg cccacgaaag gagagattag ggcaacagat tccgaagcgg gctggccgag
     3541 tattggttat aaatctgagc gattggtctt tcccgacaat cttgtcgcca gccaatacct
     3601 cgagatgatc gcaggcctct gcagtatcaa gcgagcacaa cgagataacg ttgtgctgga
     3661 aagtctggct cgtgtcaatc ttctggcagc cgcgaacaaa caaatcaagt ccctgtcaaa
     3721 agggatgcgc cagcgtcttg gattggccca ggcgatcatt ggcgatcctg atctggtgct
     3781 tctcgatgag ccatccagcg gcctggaccc ggaagggcaa gacgagatgc a
//
LOCUS       DAOMQG010000334         3824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9884_length_3824_cov_7.107721, whole genome shotgun sequence.
ACCESSION   DAOMQG010000334 DAOMQG010000000
VERSION     DAOMQG010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9884_length_3824_cov_7.107721"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..990
                     /gene="yqeB"
                     /locus_tag="PLC06_14075"
     CDS             154..990
                     /gene="yqeB"
                     /locus_tag="PLC06_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HRO91621.1"
                     /translation="MLFPHHLILLRGGGDLATGVAYRLHHAGFPVVVTELPNPLVVRR
                     RVALATAALEGEITVESLRAVRVETLDEAWTLAATGVIPVLVAPDISLLLNSSLVTRH
                     SSFVIVDARMAKRNIDTRITDAPLVIALGPGFTAGVDCHAVIETNRGHCLGRVIWRGA
                     AEANTGTPGIIAGKGRERVLRATAPGTVSWRVEIGDWVREGERLGDVAGQPVVAPFDG
                     VVRGLIAPGTVVPVGLKIGDIDPRDDISAAFTISDKALAIGGGVLEAILIALSNSERD
                     ER"
     gene            1098..2042
                     /locus_tag="PLC06_14080"
     CDS             1098..2042
                     /locus_tag="PLC06_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRO91622.1"
                     /translation="MKHRTRLIVLIVFILLAVAYGAAGWYFSSLIIASDTETLAEAAA
                     EAEADNETPADFGLPEPEVISINTGQVTLAGWYFDNPADGDCGLMFLHGFHGTRYHVL
                     NWAPIFWRRGCDILAYDHRGHGDSTPAFHTYGYYEKEDAVAALDWFTARSGLERSQIG
                     VFGVSYGAATALQMAPMTPDIAFVAADSAYSELAEIVDFQGREQFPTLAPLVLPAALR
                     IAELRANFDVEAVAPERSVADIKVPVLLIHSLTDEYTLSTHSEDIYANSDKSRTVLHL
                     NDWGAPHARDISTDPAAYEQLVDNFLAHVPNFGLVPTP"
     gene            2039..3400
                     /gene="yqeC"
                     /locus_tag="PLC06_14085"
     CDS             2039..3400
                     /gene="yqeC"
                     /locus_tag="PLC06_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HRO91623.1"
                     /translation="MTDRRWSLPAALGIGERPELVAIVGAGGKSSLMFALAAVLPGRV
                     VMTTTTRIFAAQTKHAPAVVHADDLAPLAEALAAHGRCLVIGRVEGDKALGVAPALPT
                     RLLARDDVDYVLVEADGSRMRPIKAPATHEPVIPPTTTLLVPMAGLDALEGMLEDVAH
                     RPEQIEAIMNDESGIMNEEGAWVVDGRLTAAGLAGVLAHPRGGLKDVPAGARVVPFLN
                     KADTAERLTAAREAAALLLREPRVQRVLIGALRSEHPVREVWRRVAAVVLAAGESRRM
                     GANKLLLPWGDATVLERTLSHVAASGVSSILAVVGHQRELTEPLAGRYGPIIYNGDYT
                     DGMLSSVKAAVRAFPPTVEAALIMLGDQPLVGPDIIDALLGAYAANPHGLIAPVHHGR
                     RGNPVIIDRRHFAELLALPAESAPRALLQRHPDDLLLVEVGSDAILHDLDRPEDYERL
                     RLR"
     gene            3440..>3824
                     /locus_tag="PLC06_14090"
     CDS             3440..>3824
                     /locus_tag="PLC06_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRO91624.1"
                     /translation="MMKESSGEPDWPVKVIRSSQRRKTVGARLEDGVLVIRAPASISD
                     KELTPIIEGLRKRMVRRARVVPQTDEMLEKRARELNTKYFGGRLKWQSVRYVKNQNSR
                     FGSCTPADGTIRLSDRLATMPTWVRD"
BASE COUNT          660 a         1341 c         1159 g          664 t
ORIGIN      
        1 cgcgcggcat ggccgaaatg cccttcatgc ccctcgcccc ggccgtcgtc gccgcactcc
       61 acgacgcgac gggggtttgg tttgatgaga ttccgttgtt gccgtggaag gtggtggagg
      121 gattgagggg gaatcgctta taattaacct gccatgctct tccctcacca cctcatcctc
      181 ctccgcggcg gcggcgacct ggccaccggt gtggcttacc gcctccacca cgccggcttc
      241 cccgtcgtcg tcaccgagct gcccaacccg ctcgtcgtcc ggcgtcgcgt cgccctggcc
      301 acagccgccc tggaaggcga aatcaccgtc gaatccctcc gcgccgtccg cgtcgagacg
      361 ctcgatgaag cgtggacgct ggcggcaaca ggagtgatcc cggtcctcgt cgcccctgac
      421 atatcgctac ttcttaattc gtcactcgtc actcgtcact cgtcatttgt catcgttgac
      481 gcccgcatgg ccaaacgcaa catcgatacc cgcatcaccg acgcccccct cgtcatcgcc
      541 ctggggccag gcttcaccgc cggagtcgac tgccacgccg tcatcgaaac caaccgcggc
      601 cactgcctcg gccgggtcat ctggcgcggc gcggccgagg ccaacaccgg cacgccgggc
      661 atcatcgccg gcaaggggcg cgagcgcgtg ctgcgcgcca ccgcccccgg aaccgtctcc
      721 tggcgggtgg agatcggcga ttgggtacgc gaaggagaac gcctcggcga cgtagccggg
      781 cagccggtcg tcgccccctt cgacggcgtg gtgcgtggcc tcatcgcccc cggcacagtc
      841 gtccccgtcg ggctgaaaat cggcgacatc gacccgcgtg acgacatatc ggccgccttc
      901 actatctccg acaaagcgct ggccatcggt ggcggcgtgc tcgaagccat tttaatcgcc
      961 ctgtcgaact ccgaaagaga cgagagatga gctgttctgc cgattcggtg ggcgacgtta
     1021 tcattccatg accaattcca aattactgtt gaatccaaca aattagaact gattcaggat
     1081 acgtactact attcatcatg aaacatcgca cacggttgat cgtcctcata gtattcatcc
     1141 tgctcgctgt cgcttacggg gccgccggct ggtacttctc ctccctcatc atcgcctccg
     1201 acaccgaaac cctggccgaa gcggcggccg aggccgaagc cgacaacgag actccagccg
     1261 actttggcct gcccgagccg gaagtgatca gcatcaatac cggtcaggtg acgttggccg
     1321 gctggtattt cgacaacccg gccgacggag attgcggcct catgttcctc cacggcttcc
     1381 acggcacgcg ctaccacgtc ctcaactggg cgccgatttt ctggaggcgc ggttgcgaca
     1441 tcctcgccta cgatcatcgc ggccacggcg acagcacgcc cgccttccac acctacggct
     1501 attacgagaa agaggatgcc gtcgccgcgc tcgactggtt caccgcccgc tccgggctgg
     1561 aacgctcgca gatcggcgtc tttggcgtct cctatggcgc ggccacggcg ttgcagatgg
     1621 ctcccatgac gccggacatt gcctttgttg ccgcggattc ggcctacagc gaactcgcgg
     1681 agatcgtcga tttccagggt cgcgagcagt tccccaccct cgccccgctg gtgctgcccg
     1741 cggcgctgcg cattgccgaa ctgcgcgcca acttcgatgt ggaagcggtg gcccctgagc
     1801 gctccgtcgc cgacatcaag gtgcccgtcc tgctcatcca ctcgctgacc gatgaataca
     1861 ccctctccac ccactccgag gacatctacg ccaacagcga caagagccgc accgtgctgc
     1921 atctgaacga ctggggcgcg ccccatgccc gcgatatttc caccgatccg gccgcctatg
     1981 agcaactggt cgacaatttc ctggcccacg tgcccaactt cgggctggtt cccaccccat
     2041 gaccgaccgg cgctggtcat tgcccgccgc gctgggcatc ggcgaacggc cggagctggt
     2101 cgccatcgtc ggcgccgggg gcaagagcag cctcatgttc gccttggccg cggtcctgcc
     2161 cggccgagtc gtcatgacca ccacaacccg catcttcgcc gcccagacga aacacgcccc
     2221 ggccgtcgtc cacgcggatg atttggcccc gctggccgaa gcgctggccg cccatggccg
     2281 ctgcctcgtc attggccggg tggaaggcga caaggcgctg ggcgtcgccc cggccctgcc
     2341 cacccgcctc ctggcccgcg acgacgtcga ctacgtgctc gtcgaagccg acggatcgcg
     2401 catgcggccg atcaaggccc ctgccaccca tgagccggtc atccccccaa ccacgacgct
     2461 gctcgtcccg atggccgggc tggacgcatt ggagggcatg ctcgaagatg tcgcccatcg
     2521 gccggaacag atcgaagcaa ttatgaatga tgaatcagga attatgaatg aagagggggc
     2581 gtgggtggtt gatgggcggc tcacggcggc cgggttagcg ggggtgctgg cccatccgcg
     2641 gggcggactg aaggacgtac cggccggcgc gcgggtcgtc cccttcctga acaaggccga
     2701 cacggcggag cgattgacgg cagcgcgcga ggcggccgct ttgctcctgc gcgagccgcg
     2761 cgtgcagcgg gtgctgattg gcgcgttgcg ctccgaacat ccggtgcgcg aggtgtggcg
     2821 gcgagtggct gccgtcgtgc tcgctgccgg tgaatcgcgt cgcatggggg ccaacaagct
     2881 gctcctgccc tggggcgatg caaccgtgct ggagaggacg cttagccatg tggccgcctc
     2941 aggcgtatcg tcgatcctcg cggtcgtcgg gcatcagcgc gagttgacgg aaccactagc
     3001 cggtcgatat gggccgatta tctacaatgg cgactatacc gacggaatgc tctcatccgt
     3061 gaaggctgct gtccgcgcct tcccccccac cgttgaggcg gcgctaatca tgctgggcga
     3121 ccagccgctc gtggggccgg acatcatcga cgcgctgctg ggggcctatg ccgccaaccc
     3181 ccatggcctc atcgcgcccg tgcatcacgg ccggcgcggc aacccggtca tcatcgaccg
     3241 gcgccatttt gccgaattgc tggccctgcc ggccgagtcc gccccccgtg ccctgctcca
     3301 gcgccacccc gacgacctgc tattggtcga agtgggaagc gacgcgattc tccacgacct
     3361 cgaccggccg gaagactacg agcgattacg gctgcgataa tgccttgtgc ctcccgccac
     3421 ggctgctaaa atcaacgact tgatgaaaga atcatcggga gaaccggact ggccggtcaa
     3481 ggtcattcgc agcagccaac ggcgcaagac cgtcggcgcg cggttggaag acggcgtgct
     3541 cgtcattcgc gcgcccgcgt caatcagcga caaggaactg acccccatca tcgaaggcct
     3601 gcgaaagcgc atggtccgcc gcgcccgcgt agttccgcaa acggatgaaa tgctggagaa
     3661 acgcgcccgc gagctaaaca cgaaatactt cggcggccgc ctgaaatggc agtcggtgcg
     3721 ctacgtgaag aatcagaaca gccgcttcgg cagttgcacg cccgctgacg gtaccattcg
     3781 cctgagcgac cggctggcga ccatgcccac ctgggtgcgc gact
//
LOCUS       DAOMQG010000335         3815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9919_length_3815_cov_7.921011, whole genome shotgun sequence.
ACCESSION   DAOMQG010000335 DAOMQG010000000
VERSION     DAOMQG010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9919_length_3815_cov_7.921011"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLC06_14095"
     CDS             <1..789
                     /locus_tag="PLC06_14095"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRO91625.1"
                     /translation="RPVLRFLRRNLALLIFLALSSAAMLGAYLYFSPVSGVAEVVAAG
                     SSAETLSARFVKLVPAKPVVGRVAQSPGPVRVGIISGHKGNDSGAVCADGLTEAEVVE
                     NIAVRVVSALQTRGIAADLLSEFDPRLDGYVGTALVSIHADSCDYINDQATGFKIAGS
                     GFTDSSALSICAEDAYRQATQLSFHANTITRDMTDYQAFRRIAAGVPAIIIEVGFMNL
                     DRALLTQQPDRVAGGITDGLWCYLDQIRGGISNAPPAALPPEGG"
     gene            829..2004
                     /locus_tag="PLC06_14100"
     CDS             829..2004
                     /locus_tag="PLC06_14100"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRO91626.1"
                     /translation="MTNPSPSIDRLIGEARQAIEAEREDKARPILREAARRAPDDPRP
                     WLLLAGVAETPRERRTYLAQARRLDEKAGAAPMARPAPGGYRAAAATTPAPVAPPERR
                     RRPPATPGRLMRRGMALAALVLLLALVAAMIHPDGRTVLADVVGRVSLLTGVAVVPTK
                     AVTPPNVGPTAAVVPTEVVVVEAPAGEPIEAVAPSPTGPPLFPTKDVAPGSGPSGESG
                     GGPLPTWTPTTEPTPTLAPTMTPSPTPEPSPTPEPVPLSVGQLSPRPVGVGDGERWIA
                     VNLSTQTLVAYEGDTPVFETLVSSGLPQWPTVTGQYRTYMKYESQTMNGYLLGYDYYL
                     PDVPYVMYFFEDYAIHGTYWHNSFGTPMSHGCVNVSTPDAGWLFEWAPVGTLVDVHY"
     gene            2080..2541
                     /locus_tag="PLC06_14105"
     CDS             2080..2541
                     /locus_tag="PLC06_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRO91627.1"
                     /translation="MKFWINTVSYSHVKRGIEDGITQANHGRVTNLNRVSKGDYITFY
                     SPRTDYNGGEPLQHFTAIARVIDEKPYQVEMAPTFHPWRRRVEYLPCRHVPVQPLIDS
                     LEFVKDKRHWGFPFRRGLFEIGEQDFMRIAHAMDAAIETGREPHPDEFGEE"
     gene            complement(2809..3411)
                     /locus_tag="PLC06_14110"
     CDS             complement(2809..3411)
                     /locus_tag="PLC06_14110"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRO91628.1"
                     /translation="MRLGKDLINKPIYSIDEGKLLGKVQDIYLDSTLEVVQGLYTGSQ
                     GLVRRKAEFIRSGDVVVFGADAVLVRGNDVVTDDSATPAAKEWIRREKLVGREADTPG
                     GTRLAVIGDVIVDPSGRITGFALSRTYVEGPLAEKRVISRSVVIDTGQEDGRMTVDLS
                     KLEESLLNAEAVPLAVESIGDIPASKVDPSTLFVDPSATE"
     gene            complement(3537..>3815)
                     /locus_tag="PLC06_14115"
     CDS             complement(3537..>3815)
                     /locus_tag="PLC06_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HRO91629.1"
                     /translation="FGQRAATLPISSTKSMTGHLLGAGGALEAVLCLMALRDGVIPPT
                     INYTRPDPACDLDYVPNTARAADLRVVMSNAFGLGGHNATLILGRVVS"
BASE COUNT          680 a         1217 c         1243 g          675 t
ORIGIN      
        1 cggccggtgt tgcggttttt gcgccgcaat ctggcgctgc tgatcttcct ggcgctgtcg
       61 tcggcggcca tgctgggcgc ttatctctat ttcagtccgg tcagcggcgt ggctgaggtc
      121 gtcgcggcgg gcagttcggc cgagacgctg tcggcccgct tcgtcaagct cgtgcccgcc
      181 aagccggtcg tcgggcgggt ggcgcaaagc cccggccctg ttcgggtggg catcatctcc
      241 ggccacaagg gcaatgactc cggcgcggtc tgtgccgatg ggctgaccga ggccgaagtg
      301 gtggagaaca tcgccgtgcg cgtggtgtcg gccctgcaga cgcgcggcat cgccgccgat
      361 ctgctgagcg agttcgaccc ccgtctcgac ggctatgtgg gcacggcgct ggtgtcgatc
      421 cacgccgatt cgtgcgacta catcaacgac caggccaccg gcttcaagat cgcaggctcc
      481 ggcttcaccg actcctcggc gctgtcgatt tgcgccgaag acgcctatcg ccaggcgacc
      541 cagctaagct tccacgccaa taccatcacg cgcgacatga ccgattacca ggcatttcgc
      601 cggatcgccg ccggcgtccc ggccatcatc atcgaggtcg gattcatgaa cctcgaccgg
      661 gcgctgctga cgcagcaacc cgatcgcgtg gccggcggaa tcaccgacgg cctgtggtgt
      721 tatctcgatc agattagggg cggcatatcc aacgcgccgc cggccgctct cccgccggag
      781 gggggctaat taccacggcg ctaacaggca ccgccaggct tgtgacgcat gacaaaccca
      841 tcaccttcca ttgacagact catcggcgag gcccgacagg ccatcgaagc cgaacgcgaa
      901 gacaaggcgc ggccgatctt gcgcgaggcg gcccgacgcg cgccggatga cccgcggccg
      961 tggctgttgc tggccggggt agccgagacc ccccgcgagc gccggacgta cttggcccag
     1021 gcgcgacggc tggacgagaa agccggggca gcccccatgg ctcggcccgc gccgggcggc
     1081 tatcgcgcgg ccgcggcgac cacgcccgcg cccgtcgcgc cgcccgaacg tcgccggcgg
     1141 ccaccggcca cgcccggtcg cttgatgcgt cgggggatgg cgctggccgc gctggtgctg
     1201 cttctggcgc tggtggcggc gatgatccat cccgacggcc gcaccgtttt ggccgatgtg
     1261 gtggggcggg tgtcgctgct gaccggtgta gcggttgtac caaccaaagc ggtcacgcca
     1321 cccaacgtcg ggccgaccgc ggccgttgtc ccgaccgagg tcgtcgtggt cgaagcgccg
     1381 gccggagagc cgatagaagc ggtcgctccg tcgccgaccg gcccgccgct gttcccgacg
     1441 aaggacgtgg cgccgggtag tgggccaagc ggcgaatcag gcggcggccc gttgcctacc
     1501 tggacgccga ccacggagcc gacgccgacg ctggcgccga cgatgacccc cagcccgacg
     1561 cccgaaccca gccccacgcc ggagccggtg ccgctgtcgg tggggcagtt atcgccccgg
     1621 ccggtcgggg tgggcgatgg cgagcgctgg atcgccgtca atctgagcac gcagacgctg
     1681 gtcgcctatg agggagatac gccggtgttc gagacgctgg tctccagcgg cctgccccaa
     1741 tggccgacgg tcaccgggca atatcgcacc tacatgaaat acgagagcca gacgatgaac
     1801 ggctatctgt tgggctatga ttattacctg cccgatgtgc cctatgtcat gtatttcttt
     1861 gaagattacg ccatccacgg cacgtactgg cacaacagtt ttgggacgcc gatgagccac
     1921 ggctgcgtca acgtgagcac gcccgacgcg ggttggctgt tcgaatgggc gccggtgggg
     1981 acgttggtgg atgtgcatta ttagtggcat ccgcgaagag cgcgcttcct catcgtcggc
     2041 cgtctggata atgcgtaccg gtaaggtcaa cacgcagcca tgaaattctg gatcaatacc
     2101 gtctcctaca gccatgtgaa gcgcggcatc gaagacggca tcacgcaggc caaccacggc
     2161 cgggtcacga acctgaaccg ggtgtcaaaa ggcgactaca tcaccttcta ttccccgcga
     2221 accgattaca acggtggcga gcctctgcaa catttcaccg ccatcgcccg tgtcatcgac
     2281 gagaagccct atcaggtcga aatggcgccg accttccacc cgtggcggcg acgagttgag
     2341 tatctcccct gccgccatgt tccggttcag ccgcttattg attcgcttga attcgtcaag
     2401 gataagcgtc attgggggtt tccgttccgg cgcgggctgt ttgagattgg cgagcaggat
     2461 tttatgcgaa tcgctcatgc gatggacgcc gccatcgaaa caggccggga accgcatcct
     2521 gacgagtttg gagaagaata gcgggggatg gctgtacggg tgggacaggg cggccgaacg
     2581 gccttatctt tgcagaatcc cgtcgttgcc gccttgtctc acccctttgc tgctacgagc
     2641 aaagaacaac gctgaagcgc ttaactacaa atgaagaggg cgagacaacc agcgaaccag
     2701 gtgctgatga acagacaagg ccattcgccg gttatcccgc ccgtatgatt gtcgccggac
     2761 tacggcatca accgatagac cgcgcgattg acagatgagg gttcgtggct actcggtcgc
     2821 cgagggatcg acgaacaggg tagacggatc gactttagag gcggggatat cgccgatgct
     2881 ctccaccgcc agggggacgg cttcggcgtt caacaatgat tcttccagct tggacagatc
     2941 gaccgtcatg cggccgtctt cctggccggt gtcgatgacc acgctgcggc tgatgacgcg
     3001 cttctcggcc agcggccctt cgacgtaggt gcgggaaaga gcaaagccgg tgatgcggcc
     3061 gctggggtcg acgatgacgt cgccgatcac cgccaggcgc gtgccgccgg gggtatcggc
     3121 ttcgcggccg accagcttct cgcggcgaat ccactccttg gcggcgggcg tcgcgctgtc
     3181 gtcggtgacc acgtcattgc cgcgcaccag gacggcatcg gcaccgaaca ccaccacgtc
     3241 gcccgaacgg ataaactccg ccttgcgccg caccagccct tgcgagccgg tgtagaggcc
     3301 ctgcacgacc tcaagggtgc tgtcgaggta gatgtcctgc accttgccca gcagcttgcc
     3361 ctcatcaatc gagtagatgg gtttgttgat taaatctttt cctaaacgca tgtgaactct
     3421 ccctcagaat gaatgaattg attgtacatc gggttgggtg ttaaggcacg atctgtagag
     3481 caagccgccg gcttgcattt tcgatctgta gagcaagccg ccggcttgcc ctacagttac
     3541 gaaaccaccc gacccagaat cagcgtcgcg ttatgcccgc caagcccaaa ggcgttggac
     3601 atgaccacgc gcagatcagc cgcgcgcgcg gtgttgggca catagtcgag atcgcaggcc
     3661 gggtcgggcc gggtgtagtt gatggtcggg ggaatcacgc cgtcgcgcag cgccatcagg
     3721 cagaggacgg cttcgagcgc gccgcccgcg cccagcagat ggccggtcat cgacttggtg
     3781 gagctgatgg ggagcgtcgc ggcgcgttgg ccgaa
//
LOCUS       DAOMQG010000336         3812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_9929_length_3812_cov_10.418153, whole genome shotgun sequence.
ACCESSION   DAOMQG010000336 DAOMQG010000000
VERSION     DAOMQG010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_3812_cov_10.418153"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLC06_14120"
     CDS             <1..912
                     /locus_tag="PLC06_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRO91630.1"
                     /translation="SDAVTPLAWLGALTERIKLGTAIMQMPGRTPANAAMTAMTLNQL
                     SGGRFLLGLGLSGPQVVEGWHGVSYAQPLARTREYVEIVRRIFRRESPLTFEGRHYQI
                     PYRGEDATGLGKPLKSTLVADPSIPIYLAAIGPKNVELAAEIADGWLPIFFAPERYDT
                     VFRDHVEAGLAKAGKTIDGFDIAPTVSVVINDNLDICYNMLRPFLALYVGGMGARGKN
                     FYYDLAVRYGFEEAATEIQDLYLAGDKGAAMARVPAELIDAVALVGPRERVRERLAIW
                     RNSPVTTLNMTVFDIETLRTVVELMAE"
     gene            923..1288
                     /locus_tag="PLC06_14125"
     CDS             923..1288
                     /locus_tag="PLC06_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91631.1"
                     /translation="MEAELSTLQLPPQADVRIEISVAAHLGITAQSAQRKVSKLVLDQ
                     VGNLLYGNPPNLVAGPRLLWRVPVWVSSPDRGPIGQAGILDVDAQTGEVLYSQEQLNN
                     IGEQARALAQRTSSNPDRG"
     gene            1245..1700
                     /locus_tag="PLC06_14130"
     CDS             1245..1700
                     /locus_tag="PLC06_14130"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRO91632.1"
                     /translation="MPLLNVPHQTQIEDSWCLPACVAMVCAYWGRPEFQGDVARWLQT
                     TEIGTPARNISRLKDYGFDPTYLEGSLQFLIESLRQSIPCILFLRTGELPYWEIDTAH
                     AIVVIGLDDQEIFLNDPFFEDAPKAVPVEEMLLAWSSFDYAVATLTPTN"
     gene            1715..3364
                     /locus_tag="PLC06_14135"
     CDS             1715..3364
                     /locus_tag="PLC06_14135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HRO91633.1"
                     /translation="MQNEISVREAEWLDRNQPIDVAGREALLIELIHALVALRPLTGK
                     RYNRTMQTFARRGLPWLSKGQVLAAYEQLCTDGRLEFDPDVRAHLQMKPMRTQSGVSV
                     VTVLTKPYPCPGQCIFCPTDVRMPKSYLHDEPGAQRAERHHFDPYAQTASRLRALERI
                     GHPTEKIELLILGGTWSSYRRDYQEWFVKRCFDALNGFEAASLAEAQTANATAQRRNV
                     GLVVETRQDRVDIDELRWFRTLGVTKVQVGIQTLDERVLTLNKRGHDVQSTRDAFRLL
                     RLAGFKIHGHWMANLLGATVASDIEDFGRLWSDPAIRPDELKLYPCMLLPNAELHDYW
                     LRGEYTPYTEEEVVEVLAECKAQVPRYVRLNRVVRDFPTTNVVAGNKKANLRQIAQEK
                     LREEDRPCRCIRCREIRRDAIYFEDLELRDLVYQTDATTEHFLSYETTAGFPHEDRLA
                     GFLRLSLPDPGIDHPLPELIGHAMIREVHVYGPALNLGDDSRGEAQHIGLGTRLIERA
                     KAIARAGGYRHMAVISAIGTREYYARLGFELNGLYMTCALD"
     gene            3413..>3812
                     /locus_tag="PLC06_14140"
     CDS             3413..>3812
                     /locus_tag="PLC06_14140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO91634.1"
                     /translation="MNPKAPISATSLPKSSIPILIREARPEDAAALIAHFRQIFAEPG
                     INLITEADEFSPPVESESRVIRQMASSPNSLFLVAEIDDRIVGQLTLEGGKRRNVQHT
                     AVLGITVANGWRGRGVGHRLIDYAIRWARES"
BASE COUNT          768 a         1206 c         1123 g          715 t
ORIGIN      
        1 tccgacgccg tgacgccgct ggcctggctg ggcgcgctga cggagcgcat caagctgggc
       61 acggccatca tgcagatgcc gggccgcacc ccggccaacg cggccatgac ggccatgacg
      121 ctcaaccagc tctccggcgg ccgctttctg ctgggcctgg gcctctccgg gccgcaggtg
      181 gtcgagggct ggcacggcgt cagctatgcc caaccgctgg cccgcacccg cgaatacgtg
      241 gagatcgtac ggcgtatctt ccgtcgcgaa tcaccgctga ccttcgaagg gcggcactac
      301 cagatcccct accgcggtga ggacgccacc gggctgggca agccgctcaa gagcacgctg
      361 gtcgccgacc catctatccc catctattta gctgccatcg gcccgaagaa cgtcgagttg
      421 gcggccgaga tcgccgacgg ctggctgcct atcttcttcg cccccgaacg gtatgacacc
      481 gtcttccgcg accacgtgga agccgggttg gccaaggcgg gcaagacgat cgacggcttc
      541 gacatcgccc ccaccgtctc cgtggtcatt aacgacaatc tcgacatctg ctacaacatg
      601 ttgcggccgt ttttggcgct ctacgtcggc ggcatgggcg cgcgcggcaa aaacttctat
      661 tatgatctgg ccgtgcgcta tggattcgag gaagcggcga ccgagattca ggacctctac
      721 ctggccggag acaagggcgc ggcgatggct cgcgtgccgg ccgagttgat cgacgccgtg
      781 gccctcgtcg gcccgcgcga acgcgtccgc gaacggctgg cgatctggcg caacagcccg
      841 gtgacgacgc tcaatatgac tgtgttcgac atcgagacgt tgcgaactgt ggtagaattg
      901 atggcagagt gaggtatgaa ccatggaagc tgagttatcg actcttcaac tcccaccgca
      961 agccgacgtt cgcattgaga tcagcgttgc cgcgcacttg gggattaccg cccagtcagc
     1021 acaacgcaag gtcagcaagt tagttctgga tcaggtgggc aatctcttat atggaaatcc
     1081 gccaaacctg gttgcagggc cacgtttgct gtggcgtgtt cctgtatggg tctcctcgcc
     1141 cgatcgcggc cccatagggc aggccggaat tctggacgtg gatgcccaga cgggcgaggt
     1201 cctttattct caagaacagc taaataacat aggcgaacaa gctcgtgccc ttgctcaacg
     1261 tacctcatca aacccagatc gaggatagtt ggtgcttgcc cgcttgtgta gcgatggtgt
     1321 gtgcctattg ggggcgaccc gaattccagg gcgacgtcgc ccgctggttg caaacgactg
     1381 aaattggcac acccgccaga aacatcagca ggctgaaaga ttacggtttc gacccaacgt
     1441 atctcgaagg atcattacaa tttctaatcg agtcacttcg acaaagcatt ccctgcatcc
     1501 ttttccttcg aaccggcgaa ctaccgtatt gggagatcga taccgcccat gctatcgtcg
     1561 taattggtct tgatgatcaa gaaatctttc tgaacgatcc atttttcgag gacgctccga
     1621 aagcagtacc tgtcgaagag atgctgttgg catggtcttc atttgactac gctgtcgcga
     1681 ccttaacgcc gacgaactaa cgatattttc cacaatgcaa aacgagatct cagttcgaga
     1741 agcggaatgg ctggatcgca accaaccaat tgacgttgcc ggtcgcgaag cgctgctgat
     1801 cgaattgatc cacgccctgg tggccttgcg gccgttgacc ggcaagcgct ataaccgcac
     1861 gatgcaaacc ttcgcccggc gcggcttgcc gtggctctcc aaagggcagg tgctggccgc
     1921 ctacgagcag ttgtgcaccg acggccggct ggaattcgac cccgacgtcc gggcgcattt
     1981 gcagatgaag ccgatgcgca cgcagtcggg cgtgtccgtc gtcacagtgc tgaccaaacc
     2041 ctacccctgc cccggccagt gcatcttctg ccccaccgac gtgcgcatgc ccaagagcta
     2101 cctgcacgac gaaccgggcg cgcaacgcgc cgaacgccac cacttcgatc cctatgccca
     2161 aaccgcgtcg cgtttgagag cgctggagcg gatcggccat cccacagaga agatcgagct
     2221 gctcatcctg ggtggcacct ggtcgagcta tcggcgcgac tatcaggagt ggtttgtcaa
     2281 gcgctgcttc gacgcgctga acggcttcga ggcggcctcg ctggccgagg cgcagacggc
     2341 caatgccacc gcccaacggc gcaacgtcgg cctggtagtt gagacgcgcc aggatcgcgt
     2401 cgatatagac gagctgcgct ggtttcgcac gctgggcgtg accaaggtgc aggtcggcat
     2461 ccagacgctc gacgagcgcg tgttgaccct caacaagcgc ggccacgacg tgcagagtac
     2521 ccgcgacgcc ttccggctgc tacgtctggc gggattcaag atccacggcc actggatggc
     2581 caatctcctc ggcgcgaccg tggcgagcga tatcgaggat ttcggccgcc tgtggagcga
     2641 tccggccatt cgccccgacg agctgaagct ctacccctgc atgctgctgc ccaatgccga
     2701 actccacgac tactggctgc gcggtgagta tacgccctac acggaagaag aagttgtcga
     2761 ggtgctggcc gagtgcaagg cccaggtgcc gcgctacgtc cggctcaacc gcgttgtccg
     2821 cgacttcccg acgaccaacg tggtagccgg taacaagaag gccaacctgc gccagatagc
     2881 tcaggaaaaa ctacgcgaag aggaccgacc gtgtcgttgc attcgctgtc gcgaaattcg
     2941 gcgcgatgcc atctacttcg aagacctcga attgcgcgat ctggtctatc aaacggacgc
     3001 gacgaccgag catttcctga gctacgagac gaccgccggc tttccccacg aggatcggct
     3061 ggcgggcttc ctgcgcctgt cgttgcccga tcccggcatc gaccaccccc tgcccgaact
     3121 gatcggccac gccatgatcc gcgaggtcca cgtctatggc ccggcgctca acctgggcga
     3181 cgacagccgg ggcgaagccc aacatatcgg cctggggacg cggctcatcg agcgggccaa
     3241 agcgatcgcc cgcgccggcg gctatcgaca catggcggtc atcagcgcta tcggcacgcg
     3301 cgaatactac gcgcgtttgg gatttgagtt gaacggtctc tatatgacct gcgcgctgga
     3361 ttaggtttcg taccgatcga aatatcgctt ctatgccggt gaaagcaaac caatgaaccc
     3421 gaaggccccc atctccgcaa catcgttgcc taaaagttcg atccccatac taatccgcga
     3481 ggcgcggccg gaagacgcgg cggcgctcat cgcccatttt cggcagatct tcgccgaacc
     3541 gggcatcaat ctcatcaccg aagccgacga gttttcccca ccggtagagt ccgaatcccg
     3601 cgtcatccgc caaatggcaa gctctccaaa cagcctgttt ctggtggctg aaatcgatga
     3661 tcgcatcgtt ggccaactga cgctggaagg cggcaaacgg cgcaatgtgc agcacacggc
     3721 cgtgttgggc atcactgtgg cgaacgggtg gcgcggtcgc ggcgtcggcc atcgcctgat
     3781 tgactacgcc atacgatggg cgcgggaatc cg
//
LOCUS       DAOMQG010000337         3788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10006_length_3788_cov_11.643182, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000337 DAOMQG010000000
VERSION     DAOMQG010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10006_length_3788_cov_11.643182"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLC06_14145"
     CDS             <1..1252
                     /locus_tag="PLC06_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HRO91635.1"
                     /translation="STHDCCNDHVVGIMITPALRTLSFYAFLFAALFSPLWISPVLAQ
                     DEQIRIQSRADYIYGQSMNFHLLAENLGEIETATLFIRLGTSPDSFAVDIPVEVGKRI
                     ELSYELDLTQTRLPPFGSITYWWDLDRVGDSTIRVPEQIISYVDDQFSWRQLVTTDEQ
                     GGGSVRIHWTGENESLGELARNLTFGMLPEIGRFAPITQILPFDVYIYPSSADLSSAL
                     RLAGRDYQPGETFPDLGVVLLSVVNPETAESELRSGLSIGLTDLLLYQAMNQSAYNVP
                     AWLRYGIAGIVRGRSDAVAEDSLISAIVAGETLPISELCSGTAIEDSLMIAQSESVVR
                     HIIATDDENAVRDLVVAFAQGKDCPTALRSVIQLTPEQLESSWLRANSGNQSSRTAAE
                     IGVWLVLVLAGFGLAGLLLLRPRR"
     gene            1384..2778
                     /locus_tag="PLC06_14150"
     CDS             1384..2778
                     /locus_tag="PLC06_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRO91636.1"
                     /translation="MTVQLAHFTPFHERMMAEQLPEIFINSFAHYYEKLVAGEDGMIP
                     EDSIIPIDTLMDADVLPASLDMVGREAVSRTVVIKLNGGLGTGMGLTGPKSLLKVKDG
                     LSFLDIIARQAIGIGSPLILMNSFATDGASLDVLSRYPALQHELPPSFLQHKEPKVSA
                     VDFSPACYPDDRDLEWCPPGHGDLYIALATSGMLDEMLAAGFEYAFVSNADNLGAVLD
                     FRILGHMVSCGLPFLMEVADRTPADRKGGHLAQHVDGQLVLREIAQCPADDMAAFQDI
                     GRHRYFNTNNLWLHLPTLQSVLIERDYHLGLPMIRNRKTIDPRDPDSMPVYQLETAMG
                     SAIEVFGGAAAIRVPRTRFAPVKKTEDLLAVRSDAYVLTPDNRVVLARERRGTPPTVE
                     LIGDGYRFVSDLDARFPAGVPSLRRCNRLVVEGDFRFGRGIILRGDVHFRNTNGKCKT
                     VPDGLSLEDQEWPG"
     gene            complement(2883..3611)
                     /locus_tag="PLC06_14155"
     CDS             complement(2883..3611)
                     /locus_tag="PLC06_14155"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HRO91637.1"
                     /translation="MQAIIKIRRFNPETDKKPWWGEYTLENVHRTDTVLDLLHRIKWE
                     QDGTLALRRSCGHGVCGSDAMRINGANALACKTLVNRVGGGGKDGVIKIQVEPILGLT
                     VEKDLIVDMEPFFDHYRSVMPYFINDQPVPADGRERLQSPEDRARFDDTTKCILCAAC
                     TSSCPSFWADDKYIGPAAIVQAHRFIFDSRDEHLDERLEVMADTFGVWKCRTIFNCTN
                     ACPREIQVTEAIAEVKQAIVSGKV"
     gene            complement(3624..>3788)
                     /locus_tag="PLC06_14160"
     CDS             complement(3624..>3788)
                     /locus_tag="PLC06_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91638.1"
                     /translation="RKESRGGHARDDYPERNDEEWLKHTLCTRAADGTYSLDYKPVVL
                     GRYEPKKRVY"
BASE COUNT          792 a          963 c         1095 g          938 t
ORIGIN      
        1 gtccactcac gattgctgca acgatcacgt tgttgggatt atgatcacgc cggcgcttcg
       61 cactctctcc ttttacgcat tcctgtttgc ggcacttttt tcgcccttgt ggatcagccc
      121 tgttttggcc caggacgaac agatccgtat ccagtccaga gctgattata tctatggcca
      181 atcgatgaat tttcatctgt tggcggaaaa cttgggcgag atcgaaactg cgacgctgtt
      241 catacgcctg gggacatcgc ccgattcatt tgccgttgat attccggttg aggttgggaa
      301 gcgaattgag ctctcatatg agctggacct gacacagaca cgtttgcccc ccttcgggtc
      361 gattacctat tggtgggatc tggatcgagt cggcgattcc acgattaggg ttcccgagca
      421 gataatcagt tatgtggacg atcagttcag ctggcggcag ttggtgacca ctgacgagca
      481 gggcgggggg agcgtccgca ttcattggac gggagagaat gagtcgctgg gcgaactggc
      541 gcgcaattta acgttcggga tgctgccgga gatcggtcgg ttcgcgccga ttacgcaaat
      601 cctgccgttt gatgtctaca tttatccgtc atcggcggat ctcagttcag ccttgcgatt
      661 ggcgggcaga gactaccagc ctggtgagac ctttcccgat ctcggcgtgg tgctgttatc
      721 tgtagtgaac cctgaaacgg ccgagagcga acttcgctcc ggtttatcca ttgggttgac
      781 cgacctgctt ctgtaccagg caatgaatca gtctgcctat aatgtccctg cctggttgcg
      841 ttatggcatt gccggcatag tgcgggggcg gagtgatgct gttgccgagg actcgttgat
      901 ctcggctatt gtggcgggcg agacgctccc gatttccgag ctgtgttcgg gtacggcgat
      961 cgaagatagc ctgatgatcg cgcaaagcga atcagtcgtg cgccacatca tcgctacaga
     1021 tgacgagaat gcggtacgtg atctcgttgt tgcctttgct cagggaaagg actgtcctac
     1081 agcgcttcgt tctgtcatcc agttgacccc tgagcaattg gaatcatcgt ggctgcgagc
     1141 taatagcggc aaccagagca gccgtacggc agctgaaata ggcgtctggc tggttctggt
     1201 gctggccggg tttgggttgg ccgggctgct cttgctacgc ccacggcgct gaacgatcta
     1261 tgcttaaggg aattaataag tctttgcgga tataatgacc tgttaggtgt tcaggtaact
     1321 acaatccaac tattgaggct ttacagcagc gccccacgga ttagccttgg taaagagaat
     1381 tttatgacgg tacaactggc tcacttcact ccgttccatg aacggatgat ggctgaacaa
     1441 ctgccggaga tttttatcaa tagcttcgcc cattattacg agaaattggt ggcgggtgag
     1501 gatggaatga tcccggaaga cagcattatt cccatagata ctctcatgga tgcagatgta
     1561 ttacccgcgt ctcttgacat ggtagggcgc gaggcagtca gcagaacagt tgtcatcaag
     1621 ctgaacggcg ggttgggtac gggtatgggg ttgactggcc ccaaatcgct gctcaaggtc
     1681 aaggatggat tgtcatttct tgacataatt gctcggcaag cgatagggat tggatcaccc
     1741 ctaatcctga tgaacagctt tgccacggat ggcgcatcac tggacgttct ctctcgttat
     1801 ccggcactac agcacgagtt gccacccagc ttcttgcagc ataaagagcc taaggtgagt
     1861 gcagtagatt tcagcccggc ctgctatcca gatgatcggg atttggagtg gtgcccaccc
     1921 ggacatggcg acctgtatat tgccctggcc acaagcggaa tgcttgatga gatgctggcc
     1981 gcaggattcg aatacgcttt tgtctcgaat gccgataatc tcggcgcggt gctcgatttc
     2041 agaatccttg gccatatggt gagctgcgga ctacccttcc tcatggaagt ggcggatcgg
     2101 actccggcag accgaaaggg ggggcatctg gctcagcatg ttgatggaca acttgtcctg
     2161 cgcgagattg cgcaatgccc tgcggatgac atggctgcat tccaggatat cggccgccat
     2221 cgttatttca acaccaacaa cctatggtta catttgccga cactgcaaag cgtattgatt
     2281 gagcgagatt atcatcttgg gctgccgatg attcgaaacc ggaaaacgat cgatccccgc
     2341 gatcctgatt cgatgccggt ttatcaattg gagacggcca tgggctcagc tatcgaagta
     2401 tttggaggag ctgccgcgat tcgggtgccc agaacacgct tcgcgccggt gaaaaagacg
     2461 gaagatctgc tggctgtgcg gtcagatgct tacgtgttga cgccagataa tcgggtggtg
     2521 cttgcccgcg agcggcgtgg cacgccgccg actgtggagt tgattggcga cggatatcgc
     2581 tttgtgagcg atttggacgc tcgtttcccg gccggggtgc cttcgctgcg acgttgcaat
     2641 cgactggtgg tggaaggcga ttttcggttc gggcgcggga ttattttgcg tggcgatgtt
     2701 cacttccgta atactaacgg caagtgcaaa accgtccccg atggcttgtc ccttgaggat
     2761 caggaatggc cgggctgatc atggtccagt agttcattaa ttcctgttaa ataataaagg
     2821 cgcaccgaat ctggtgcgcc tttatttatt atgtgatcaa tcttcccgga aggaagttgt
     2881 ggctaaacct tcccggagac gatggcctgt ttgacttccg cgattgcctc ggttacctgg
     2941 atctcgcggg ggcaagcatt tgtgcagttg aagatcgtgc ggcacttcca tacaccgaat
     3001 gtatcagcca tcacttccag ccgttcatcc aggtgctcgt cgcgggaatc aaagatgaag
     3061 cggtgggcct gaacgatggc ggctgggccg atatatttgt cgtcggccca gaagctgggg
     3121 cacgatgacg tgcaggccgc gcagaggatg catttagtgg tgtcgtcaaa tcgcgcgcga
     3181 tcctcaggcg actgcaatcg ctcgcgtcca tcggccggca caggctggtc gttgatgaaa
     3241 tagggcatga ccgagcgata atgatcgaag aacggttcca tgtcgacgat gaggtctttc
     3301 tcgacagtca gccccaaaat cggctcgacc tgaattttaa tgacaccgtc cttgccgcca
     3361 cccccgacgc gattaacaag cgtcttgcaa gccagcgcgt tggctccatt gatacgcatg
     3421 gcatccgaac cgcagacgcc atgcccgcaa gatcgtcgaa gagcaagcgt gccgtcctgc
     3481 tcccacttaa tgcggtgcag cagatcgaga acggtatcgg tccgatggac attttccaga
     3541 gtatattcgc cccaccaggg cttcttgtca gtttccgggt tgaagcgccg gattttaatt
     3601 atggcttgca tcgtcatgac ctcttaatag acacgcttct tcggttcata gcggccgagc
     3661 acaacgggtt tgtagtcgag agagtaagtg ccgtctgccg cacgggtgca cagggtgtgc
     3721 ttcagccact cttcatcatt gcgttcagga taatcatcgc gagcgtggcc cccgcgactt
     3781 tccttgcg
//
LOCUS       DAOMQG010000338         3770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10060_length_3770_cov_8.976581, whole genome shotgun sequence.
ACCESSION   DAOMQG010000338 DAOMQG010000000
VERSION     DAOMQG010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_3770_cov_8.976581"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLC06_14165"
     CDS             <1..924
                     /locus_tag="PLC06_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRO91639.1"
                     /translation="DKVAHYSGPWSRHAFGTLRGFDRFVGQLRTIIAEKAPRPYELLI
                     LSDHGQSSGATFKMRYGYTLLEFIERYLPSDTVVSAAYDHDDGSTSVVAMAAELSNIQ
                     EQGMGGRIGKVMTEGMQELAEEATEELEEREDEDLKTAADVTFCASGNLAQVYFHAFP
                     QRATMNELNHAYPNLIEALLRHEGVGLVVVDDDEGVPLALGPHGARNLHTGVVSGEDP
                     LTLYGDAELRAWQTRRISDYPCAGDLTIISTVYPDNTVAAFEELIGVHGGLGGEQTDS
                     FLLHPATMTVSPTRSSTDVFGILNARREPLM"
     gene            1024..3705
                     /locus_tag="PLC06_14170"
     CDS             1024..3705
                     /locus_tag="PLC06_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRO91640.1"
                     /translation="MSIIRTILRTAVRFFIIWVVSALALIVANFLPIGITIHPVDGLS
                     IWPVALAAALVLGLVNVLIRPLILLLALPLGFFALFIVALFVNAVTLRITALLFSDGL
                     DVAGWLSAFIGGVVIGSAISLISAILGIGDTGSFYESIIERRLRAQRDAVIGDASSGL
                     VMLEIDGLSYHHITRAVAAGYMPNVAALIKRQSYALSLFDCGLPSQTSACQAGILFGD
                     NYDIPAFRWYDKAQGRLFVSGRDAAAINARFAGDGGLLRGGASIGNLVDGDAAISLLT
                     AADLRGGTPEQKLARAHDIYLLLLNPDFFMRVVGLYIGETLLEVWQYLRDVLTDTRPR
                     LNRLNNFYPFVRASATVFMREVTALLTGMQIVRGEPAIYTTWPGYDEVAHHSGPWSRH
                     ALGTLRGFDRTVGSIRRIIATKAPRPYELILLSDHGQSFGATFRMRYGYTLETFIHQR
                     MPAGTVMAREAGGDDGSQVAASAAAELGNVREQGMGGRVGQATTRQLQRAVERGVPDA
                     EQAPAAEPAPRADVTFCASGNLGQVYFHAFPHRATLGELNAAFPGLVDEIAAHEGVGF
                     VVGLGAEGRPIAIGKDGTRDLATGDLRGGDPLAPYGDPTLRAWQVRRVADFPSAGDLI
                     IMSAIYPDGTVAAFEELIGNHGGLGGEQTDAFILHPADWVVPPTRSATDLFPVLDARR
                     ERVVPQSRSAAPRRERDDWSLSNLAAGLRDVGAWVELAFYGTILDRAAYRAIATQGAM
                     TGPAILLSLAGVLVSTLMETPPADAAELARHFLTRYGLWVLAVLLIQGAAHVLRGKAN
                     FTQTFRVMGFAEAASLLGLLGLLPLIGPFALTAASILSLFVSWLSLSEAHGFRGRRTL
                     LLPVLYFVVVIAGDAYLSGLAGGFKLALDVLMGLLRP"
BASE COUNT          573 a         1232 c         1244 g          721 t
ORIGIN      
        1 gacaaggtgg cccattactc cgggccgtgg tcgcgccacg ccttcggcac gctgcgcggc
       61 ttcgaccgct tcgtaggcca attacggacc atcatcgccg aaaaagcccc ccggccctat
      121 gagttgctca tcctgtccga ccacggccaa tcgtcggggg cgacgttcaa aatgcgctac
      181 ggctacacgc tgctggagtt catcgagcgc tatctgccca gcgacacggt cgtatcggcc
      241 gcctacgatc atgacgacgg ctcgacgtcg gtggtggcca tggccgccga actgagcaac
      301 atccaggagc agggcatggg cggccgcatc ggcaaggtca tgaccgaggg gatgcaggaa
      361 ctggccgagg aagcaaccga agagctggag gagcgcgagg acgaggatct gaaaaccgcg
      421 gccgacgtca ccttctgtgc cagcggcaat ctggctcagg tctatttcca cgccttcccc
      481 caacgggcga cgatgaacga actgaaccac gcctacccta acctgatcga agcgctgctg
      541 cggcacgagg gcgtcgggct ggtggtggtt gacgacgacg agggcgtgcc gctggctctg
      601 gggccgcatg gcgcgcgcaa ccttcatacc ggcgtcgtga gcggcgaaga cccgctgact
      661 ctctatggcg acgccgagct gcgcgcctgg cagacgcgac gcatctccga ttatccctgc
      721 gccggtgacc tgacgatcat cagcaccgtc tatcccgaca acaccgtcgc tgcgttcgaa
      781 gaactcatcg gcgtccacgg cggcctgggc ggcgagcaga ccgacagctt cctgctccat
      841 ccggcgacca tgaccgtgtc gccgacgcgc agttcgaccg acgtgttcgg catactcaac
      901 gcccggcgcg aaccgctcat gtgagcggca tggcggcttg aattccgggc ggctgccctg
      961 ttgataagcc cgcgttttca tggaagcagt gtgaccggcg atagttccgg ccttgcatgg
     1021 cccatgagta tcattcgtac catccttaga acggcagttc gcttcttcat catctgggtc
     1081 gtcagcgctt tggcgctcat cgtcgccaat ttcctgccga tcggcatcac gatacacccg
     1141 gtcgatgggc tttcgatatg gccggtggct ctggccgcgg cgcttgtcct ggggctggtc
     1201 aatgtgctga ttcggccgct catcctgctg ctggccctgc cgctgggttt cttcgccctg
     1261 ttcatcgtcg ccttgttcgt caacgccgtc acgctgcgca tcacggcgct gctgttttcc
     1321 gatggtctcg acgtggccgg ctggctttcg gcgttcatcg gcggcgtcgt catcggctcg
     1381 gccatctcgc tcatcagcgc catcctcggc atcggggata ccggctcatt ttacgagagc
     1441 atcattgagc ggcgattgag ggcgcaacgg gacgcggtga ttggcgacgc ttcttccggg
     1501 ctggtcatgc tggagatcga cggcctgagc taccaccaca tcacgcgggc cgtggccgcc
     1561 ggctacatgc ccaatgtcgc ggcgctgatc aagcgtcaga gctatgcgct gtcgctcttc
     1621 gattgtggcc tgccctcgca gacgtcggcc tgtcaggcgg gcatcctgtt cggcgataat
     1681 tatgacatcc ccgcctttcg ctggtatgac aaggctcagg gcaggctttt cgtgtccggc
     1741 cgggatgcgg cggcgatcaa cgcccgtttc gctggtgacg gcgggctgct gcgcggcggc
     1801 gcgagtatcg gcaatctggt ggacggcgac gcggccatct cgctgctgac ggcggccgat
     1861 ctgcgcgggg gcacgccgga gcagaagctg gcccgcgccc atgacatcta cctgctgctg
     1921 ctgaaccccg acttcttcat gcgcgttgtc ggcctctata tcggcgagac gttgctcgaa
     1981 gtatggcaat acctgcgcga cgtcctgacc gacacgcggc cgcggctgaa tcgcttgaac
     2041 aacttctacc cgttcgtgcg cgcttcggcg acggtcttca tgcgcgaggt gacggccttg
     2101 ctgaccggca tgcagatcgt gcgcggcgaa ccggccatct acaccacctg gcccggctac
     2161 gacgaggtgg cccatcactc cgggccgtgg tcgcggcatg cgctgggcac gttgcgcggc
     2221 ttcgaccgca cggtcggctc gatccggcgg atcatcgcca ccaaagcccc gcgcccctat
     2281 gagctgattc tgttgtcgga tcacggccag tccttcggag ccaccttcag gatgcgctac
     2341 ggctacacgc tggagacgtt catccatcaa cgaatgcccg cgggcacggt gatggcgcga
     2401 gaggccggcg gcgatgatgg ctcgcaggtg gccgccagcg ccgccgccga attgggcaac
     2461 gttcgcgagc agggtatggg cggccgcgta ggtcaggcca ccacccggca gttgcagaga
     2521 gcggttgagc ggggcgtccc ggatgccgag caagcgccgg cggccgaacc cgccccgcgc
     2581 gccgacgtca ccttctgcgc cagcgggaac ctgggccagg tctacttcca cgccttcccc
     2641 catcgcgcca cgttgggcga gctgaacgcc gcgttccccg gcctggtcga tgagattgcc
     2701 gcccacgagg gggtgggctt tgtggttgga ttgggcgcgg aagggcggcc gatcgccatc
     2761 ggcaaggacg gcacgcgcga tctggcgact ggcgacctgc gcgggggcga cccactggcc
     2821 ccctatggcg acccgacatt gcgcgcctgg caggtgcggc gcgtggccga tttccccagc
     2881 gccggcgact tgattatcat gagcgccatt tatcccgacg ggacggtggc agcgttcgag
     2941 gaactgatcg gtaaccacgg cgggctgggc ggcgagcaga ctgacgcgtt catcctgcat
     3001 ccagccgatt gggtcgtgcc gccgacgcgc agcgcgaccg acctcttccc cgtactggac
     3061 gcgcggcgcg aacgagttgt gccccaatct cgcagcgccg ccccgcgccg ggagcgtgac
     3121 gattggtcgc tctccaacct ggcggcaggc ttgcgcgacg ttggcgcgtg ggtcgagctg
     3181 gctttctacg gcacgattct cgatcgcgcg gcctatcgcg ccatcgccac acagggagcc
     3241 atgaccggcc cggccatatt gctgagtctg gccggggtgc tggtcagcac gctgatggaa
     3301 acgccgcccg cggatgcggc ggagctggcc cggcacttcc tcacgcgcta cgggctgtgg
     3361 gttctcgcgg ttttgctgat tcaaggggcg gcccacgtct tgcgcggcaa ggccaacttc
     3421 acgcagacct ttcgcgtcat gggtttcgcc gaggcggcga gtttgttggg attgctgggg
     3481 cttcttccgt tgatcgggcc gttcgccctg acggcggcgt cgatcctctc gctgtttgtc
     3541 tcctggctgt ccctgtccga ggcccatggc tttcgcggcc ggcgcacatt gctgctgccg
     3601 gtgctctact ttgtcgtcgt gatcgcgggt gatgcttatc tgtccggcct ggccggtggg
     3661 ttcaagctgg ctttggatgt gctgatgggc ctgctgcgtc cgtgagacct ggcgagggca
     3721 ttaccgtagc caaaagcgtg ggcatgccct ccgataagcc gacgagatgg
//
LOCUS       DAOMQG010000339         3765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10075_length_3765_cov_4.027224, whole genome shotgun sequence.
ACCESSION   DAOMQG010000339 DAOMQG010000000
VERSION     DAOMQG010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_3765_cov_4.027224"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="PLC06_14175"
     CDS             complement(<1..1676)
                     /locus_tag="PLC06_14175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRO91641.1"
                     /translation="MLIHVPLDQIDDNPFQRRQDYGDVESLAADIEARGLLQIPRGRL
                     IYLRGEVIPDEAARGACYRIETDGFPAGLAVQLAFGHRRLRAYRSLAAAAVAGGLLKM
                     PVYVEHMTDDQMLDFVWSENQHRSDINPVEQAELLAEKLERARAAGGNQTTVAAEWNL
                     DRSTIANKLRLLELPAEVQAALRERRLSERQAQALLPVMELEAKLNGAKVVWWTPNAA
                     WVPLSPADYVGQVLAAPEAATSDAIRQYTKQAIDHAGKPLGDDFAAFDAGQGARIIQP
                     ACKGCPRRQNQSCLGLACFDAREKRYFAALPERAARETGLPVADDAADFSFSYEESQD
                     ILSDWKAGDRDGLVVGIEVGSWGARPFHDRPFVDPYAARVDWRRAIRLGRRAGAAASP
                     DNDAENFAQPSADELAGWTTAQRKADKERAGRVKQALRFYLEPLMGDETALRALMAML
                     EGYSIREALERGELPTPETLFDGLFTLAWKRVDTGNYDGTFGNRQALRRLLHAAAISP
                     DVADPADRVLRLIDIGQVALLEWNDMRNHVGYARKRLGRLREALAEFYAVP"
     gene            complement(1709..1981)
                     /locus_tag="PLC06_14180"
     CDS             complement(1709..1981)
                     /locus_tag="PLC06_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91642.1"
                     /translation="MDRYAQRRLTGRPTRNRDGWREELTDIDPERRLMAAVIWWAVAD
                     VGSTPALARRAWRYIHSDGFAADCAWIGIDPDYIRGRLPESTIIRA"
     gene            complement(2062..2838)
                     /locus_tag="PLC06_14185"
     CDS             complement(2062..2838)
                     /locus_tag="PLC06_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91643.1"
                     /translation="MTSATLRPVQEILTHVAPGVGSWPMSVAPGRPRRMRPTLGSLRE
                     THPDLPQVLATVRAWGGAFNAARRAMNDAADEGRARPPSLILSGPNGTGKTHIARAIQ
                     WSLVDAVLDEDGRADPELISPSCRWYAAADLLGRLGDERDGEGYSYAGRPGRIVGNAP
                     FVVIDDVAAELSIPFVAWPAQEAERQIRYHLFFDWCVGNRVPVVMTTNLAAAGADSDL
                     ARHVGPRAWSRLMEMVPRGFIVSLWNVPDYRLIASGREGG"
     gene            complement(2843..3223)
                     /locus_tag="PLC06_14190"
     CDS             complement(2843..3223)
                     /locus_tag="PLC06_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91644.1"
                     /translation="MPEAVKRQKARRGIKDPEPDAPAAPPALRLLHELTGYWPGDDNA
                     PLILAGLGDDPDRSALARAVELWRGSGHKINNWLGICDWYHEILRDPGWTPQARFRRG
                     GNSAAANPMQSVSKPAPGSQPVTW"
BASE COUNT          598 a         1278 c         1235 g          654 t
ORIGIN      
        1 ggtacggcgt agaactcggc caaagcctcg cgcaggcggc cgagccgctt gcgggcatag
       61 ccgacatggt tgcgcatgtc gttccattcg agaagggcga cctgaccgat gtcgatgagg
      121 cgcaggacgc gatcggccgg gtcggcgacg tcgggactaa tagccgcggc gtggagcagc
      181 cgccgcaggg cctggcggtt gccgaacgtg ccgtcgtagt tgccggtatc gacccgcttc
      241 caggccaggg tgaagagtcc gtcgaagagc gtctccggcg tgggcaactc gcctcgctcc
      301 agggcctcgc ggatgctgta gccctccagc atggccatga gcgcgcgaag agcggtctcg
      361 tcgcccatga gcggctccag gtagaagcgc agggcctgct tgacgcggcc ggcgcgctcc
      421 ttgtcggcct tgcgctgggc cgtcgtccat ccggccagtt cgtcggccga cggctgcgcg
      481 aagttctcgg cgtcattgtc cggcgatgcc gcggccccgg cccggcgtcc cagcctgatg
      541 gcccgccgcc agtcgaccct cgccgcgtag ggatcgacga aggggcggtc atggaacggc
      601 cgagcgcccc aactgccgac ctcgatgccc acgaccagcc cgtcgcgatc tccggctttc
      661 cagtcgctca ggatgtcctg ggactcctcg tatgaaaagg agaaatcggc cgcgtcatcg
      721 gcgacgggca gtccggtctc gcgggctgcc cgttccggca gagcggcgaa atagcgtttt
      781 tctcgcgcgt cgaaacaggc gaggccgagg cagctctggt tttgccgcct ggggcagccc
      841 ttgcaggcgg gctgaatgat gcgcgcgccc tgcccggcgt cgaaggcggc gaagtcgtcg
      901 ccgaggggct tgccggcgtg gtcgatggcc tgcttggtat attggcggat ggcgtcgctg
      961 gtggcggctt cgggcgcggc cagcacctgg cccacgtagt cggccggcga gaggggaacc
     1021 caggctgcgt tgggcgtcca ccagaccacc ttcgccccgt ttagcttcgc ctccagctcc
     1081 atcaccggca acaacgcctg ggcctggcgc tcggacaggc ggcgctcgcg cagggcggcc
     1141 tgcacctcgg ccggcagctc caggagccgc agcttgttgg cgatggtgga gcggtcgagg
     1201 ttccactcgg ccgccacggt agtctggttg ccgccggcgg cgcgggcgcg ttccagcttc
     1261 tcggccagaa gctcggcctg ctccaccggg ttgatgtcgc tgcggtgctg gttctcgctc
     1321 cacacgaaat cgagcatctg gtcgtcggtc atgtgctcga cgtagacggg catcttcagc
     1381 agcccgccgg caacggcagc ggcagcgagg ctgcgatagg cccgcaggcg gcgatggccg
     1441 aaggcgagct ggacggccag gcccgcgggg aagccgtcgg tctcgatgcg atagcacgcg
     1501 ccgcgggcgg cttcgtcggg gatgacctcg ccgcgcaggt agatgagccg gccgcgcggg
     1561 atctgcaaca ggccgcgggc ctcgatatcg gccgcgaggc tttcgacgtc gccataatcc
     1621 tgccggcgct ggaacgggtt gtcgtcgatc tggtcaagag gaacgtggat gagcatggtt
     1681 ggtttctcct tgtgtttgcg cgccaacgtc aggcgcgaat gatggttgat tccgggagcc
     1741 gcccccggat gtaatccggg tcgatcccaa tccacgcgca gtcggccgca aacccgtcgc
     1801 tgtggatata gcgccatgcc cgccgggcca gtgccggcgt gctgcccacg tcggccaccg
     1861 cccaccagat gaccgccgcc atgagccgcc gctccgggtc gatatcggtc agctcttcgc
     1921 gccagccgtc gcgattgcga gtcggccgtc cggtcaatcg tcgttgtgca tatctgtcca
     1981 ttcgtctcct caaaacggcg gtttggtcgt gctcggcagc tcgcaccgca ccgcgaaata
     2041 ctcccacgcg cagcagagtc atcacccacc ctcccggccg gacgcaatca gccgataatc
     2101 cggcacgttc cacagcgaca cgataaatcc gcgcggcacc atctccatca accggctcca
     2161 cgcccgcggc cccacatgcc gggccaaatc actgtccgcc cccgccgccg ccagattcgt
     2221 tgtcatcacc accggcacac ggttgccgac acaccagtcg aaaaacaggt gatagcggat
     2281 ctgccgctcg gcttcctgcg ccggccaggc cacgaacggg atcgacaact cggccgccac
     2341 gtcgtcgatg accacaaacg gcgcattgcc gactatccgg cccggccgcc ccgcgtagga
     2401 atagccctcg ccgtcccgtt cgtcgccgag ccgccccagc agatcggccg ccgcatacca
     2461 gcggcaagac ggcgagatca actccgggtc tgcccggccg tcctcgtcga gcacggcatc
     2521 aaccagactc cactgaatcg cgcgggcgat atgggtcttc cccgtaccgt tcgggccgga
     2581 gaggatcagc gacggcggcc gggcgcggcc ctcatcggcc gcgtcattca tggcccgccg
     2641 cgccgcgtta aacgcgcccc cccaggcgcg gacagtggcc agcacctggg gcagatcggg
     2701 atgagtctcc cgcaggctgc cgagcgtcgg ccgcatccgc cgcggccgac ccggcgcgac
     2761 ggacatcggc cacgagccga cgccgggcgc gacgtgggtc aaaatctcct gaacaggtct
     2821 gagtgttgca cttgtcatga tgtcaccatg tcaccggctg actgccgggc gcgggtttgc
     2881 taacggattg catcgggttc gcggcggccg agtttccgcc gcgcctgaat ctggcctgtg
     2941 gcgtccagcc gggatcgcgc aatatctcgt gataccagtc gcagatgccg agccagttgt
     3001 tgatcttgtg cccgctgccc cgccatagct ccacggcgcg ggccagcgcg gagcggtcgg
     3061 gatcgtcgcc caggccggcc agaatcagcg gcgcgttgtc gtcgccgggc caatagccgg
     3121 tcagctcgtg cagcagcctc agcgcaggcg gcgcggccgg tgcatccggc tcagggtcct
     3181 tgatgccgcg gcgcgccttc tggcgtttga ccgcctcagg cacaggctgg aatgacgcgg
     3241 ccggggtggg cgggggcgcg acagcgctct ccccctcccc ttgacggtta acaggatggt
     3301 taataggatg gttagggtga cacagtgtca cccccccatg tgacattgtg tcacccccca
     3361 ggtgatcctc tgtcactttc gtatgtgaca ccttgtcact tactgtatgt gacatcttgt
     3421 cacttaccgt atgtgacaag gtgtcacctt ccagcttgct caaaactatc tgatagatgt
     3481 tcgacgcgtc cctgcggcgg cggcggatca ggtatcccga tttgacaagc gcgtcgattg
     3541 cacggatgac ggttcgttca gacagggcgc atttgcgagc cattccgggg atagacgggt
     3601 aggcgttccc cccgtcgtca ctggcgttgt cggccagcgc cagtaacacg aaacgttgcg
     3661 ttgaatccgt tggaccgttt tcccaaacgg cagtcataac cttgacgctc attctgctac
     3721 ctcgatacca gtacaaaacc ggtcaaatgt attgctcacc ccgct
//
LOCUS       DAOMQG010000340         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10077_length_3762_cov_5.966280, whole genome shotgun sequence.
ACCESSION   DAOMQG010000340 DAOMQG010000000
VERSION     DAOMQG010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10077_length_3762_cov_5.966280"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PLC06_14195"
     CDS             <1..1328
                     /locus_tag="PLC06_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91645.1"
                     /translation="MSGLIDWSFIITLGVIVFTALLGAYMRARHEDRCLRDFDGYRVT
                     VERDGHGIVWGDLKVHPTGVELVYGVDVKDDNHMETSYIYYKDEFGDLHAIYRHARDL
                     TPELQARRARSVERSLHPGLFRRARRSLRNFLSTAADSFADALTMTMGRMRYKGANLM
                     TDTSQTYMRGISKDVFGYVGTSFDPLLEQYIGTRVVMEMVEDGVVHEHCGVFKEYSAD
                     FLELLDVYYPLPQEVNLNGITEIQIADKVTIIREEKQLRVCNKADQPQHLERIEVGGE
                     ETELNAILPGGEEMVVPIAAEGQTVDLYMRIASRLDMIVPRAGALIRHRAERYDPDSI
                     FDVGLAIVRRDTTDREIEKLRERLRYSPEDAGAAARLGEVLLLTGNFGEARQWLLEAK
                     KRAHRLPDHGNRVDQNLRQVERHIGPAKKVTSDGKVTSNEVRSTKDADR"
     gene            complement(1456..2856)
                     /locus_tag="PLC06_14200"
     CDS             complement(1456..2856)
                     /locus_tag="PLC06_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91646.1"
                     /translation="MIPRLFRQIVVPLIAPVLLMGLVLFLLLRREACLIDAVCVRNAL
                     VAAVAVGLVGALAAAWLVSSRIARPLNDVAEVARRIAAGDGTARALTQRRDEIGDLVR
                     AFSAMTEQQRERIEELSQDYRRFVAVIEHMADGVIIIDRAGDVVLINPAACRLMHTHE
                     SDALGRSFAAVVRHHALIELWQRGRREQSEQVEAVEISPKLFLQAVLTPYSRAEGAGF
                     IVLLQDLTQVRRLQVMRRDFISNLSHELRSPLASLRAVVETLQDGALDDPPAAERFLR
                     QAEGEVNTMTQMVEELTELSQIESGQIRLRLEPAAVGDLVAGPLERLRPQAEAGELAL
                     AAELPGDLPPVLVDAERVRQVMTNLLHNAIKFTPPGGRVTVRATVAEAGRMVVIEVRD
                     TGMGIPKADESRVFERFYKSDQARTRGRGGTGLGLAIARHIVETHGGRIWVKSKEGKG
                     SGFFFSVPAGEVTSDE"
     gene            complement(2864..3607)
                     /locus_tag="PLC06_14205"
     CDS             complement(2864..3607)
                     /locus_tag="PLC06_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91647.1"
                     /translation="MPYRVLLVEDDATLRETVAYNLRHQGYEVVEAGNGLMALEAARR
                     EQPDVVLLDVMLPGIDGFEVCRLLRKETNLPILMLTARSDEVDRVVGLEMGADDYLTK
                     PFSMRELMARVKALLRRAELIREELAAELRDAAPATTRQESRPPDTGALIFGDLVLDL
                     GRREVRLGGEVVGLKPREFDLLLFLARNRGIALSRDLILERVWGWTFDGNSRTVDVHM
                     RWLREKLERDPANPARLVTVRGIGYRFEG"
BASE COUNT          668 a         1299 c         1203 g          592 t
ORIGIN      
        1 ccatgtccgg actaatcgac tggtcattta tcatcaccct gggcgttatc gtcttcaccg
       61 cgctgctggg cgcgtatatg cgtgcgcggc atgaggatcg ctgcctgagg gatttcgacg
      121 gctaccgggt gactgtggag cgcgacggcc atggcatcgt ctggggcgac ctgaaagttc
      181 accccaccgg ggtcgagctg gtctatggcg tcgacgtaaa ggacgacaat cacatggaga
      241 cgagctacat ctattacaag gatgagttcg gcgatctgca tgcgatctac cgccacgcgc
      301 gcgacctgac gcccgagctg caggcccgcc gcgcccgcag tgtggagcga tcgctgcatc
      361 ccggcctgtt tcgccgcgcg cggcgctcgc tgcgcaactt cctcagcacg gcggccgatt
      421 cattcgccga cgcgctgacg atgacgatgg gccgcatgcg ctataagggc gcaaacctga
      481 tgaccgacac cagccagacc tatatgcgcg gcatcagcaa ggatgtcttc ggctatgtgg
      541 gcaccagctt cgaccccctg ctggagcagt acatcggcac gcgggtggtc atggagatgg
      601 ttgaggacgg ggtggtccac gagcactgcg gcgtgttcaa ggagtattcg gccgattttc
      661 tggagcttct ggacgtgtac tacccgctgc cgcaggaggt gaacctgaac ggcataacgg
      721 agattcaaat cgccgacaag gtcaccatta tccgggaaga gaagcagctg cgcgtctgca
      781 acaaggccga ccagccgcaa catctggagc ggatcgaggt gggcggggag gagacggagc
      841 tgaacgctat cctgcccggc ggcgaggaga tggtggtgcc aatcgcggcc gaggggcaga
      901 cggttgacct gtatatgcgg atcgcctcgc ggctggacat gatcgtgccg cgagccggcg
      961 cgctgattcg ccaccgggcg gagcgatatg acccggactc gatcttcgac gtagggctgg
     1021 cgatcgtgcg acgggatacg accgaccgcg agatcgaaaa gctgcgcgag cggctgcgct
     1081 attcgccgga ggatgccggg gcggcggcca ggctggggga ggtgctgctg ctcacgggca
     1141 acttcggcga ggcgcgccag tggctgctgg aagcgaagaa gcgcgcccat cgcctgccgg
     1201 atcacgggaa ccgggtcgat cagaatttgc gccaggtgga gcgccacatt gggccggcga
     1261 agaaagtgac gagtgacggg aaagtgacga gcaacgaagt acgaagtacg aaggacgccg
     1321 atcgctgaca gacgaccgcc ggccgccgca agagcgcgcg cccaaagtac gaaatacgaa
     1381 gtacgaagca gggcgcgcgc ttcttctttc gtacttcgta tttcgtagtt ggaacggctc
     1441 ttcattcgtc attcgtcatt cgtcactcgt cacttccccc gccggcacac taaaaaagaa
     1501 cccgctcccc ttcccttcct tgctcttcac ccaaatgcgc ccgccatggg tctccacgat
     1561 atggcgggcg atggccaacc ccagccccgt gccgccgcgg ccgcgcgtcc gcgcctggtc
     1621 ggacttatag aaccgctcga acacccgcga ctcgtccgcc ttggggatgc ccatgcccgt
     1681 gtcgcgcacc tcgatcacca ccatccgccc cgcctcggcg acggtcgccc gcaccgtcac
     1741 ccggccgccc ggcggtgtga acttgatcgc gttgtgcagc agattggtca tcacttgccg
     1801 cacccgctcg gcgtctacca gcaccggcgg cagatcgccc ggcagctcgg cggccagcgc
     1861 cagctccccc gcctccgcct gcggccgcaa ccgctccagc ggcccggcca ccagatcgcc
     1921 caccgccgcc ggctccagcc gcagccgaat ctgccccgat tcaatctgcg acagctccgt
     1981 cagctcctcc accatctgcg tcatcgtgtt gacctcgccc tccgcctgcc gcaaaaagcg
     2041 ctcggccgcc ggcgggtcgt cgagcgcgcc gtcttgcagc gtctccacca ccgcccgcag
     2101 cgaggccagc ggcgagcgca gctcatggga cagattgctg atgaagtcgc gccgcatcac
     2161 ctgcagccgc cgcacctggg tcaggtcttg cagcagcacg atgaacccgg ccccctccgc
     2221 ccggctgtag ggggtcagca ccgcctgcag aaatagcttg gggctgatct ccaccgcctc
     2281 cacctgctcc gactgctcgc ggcggccgcg ctgccacagt tcgatgagcg cgtgatggcg
     2341 caccacggcg gcgaacgaac ggccgagcgc gtcgctctca tgcgtgtgca tcagacgaca
     2401 ggcggccgga ttgatcaaca ccacatcccc cgcccggtcg atgatgatca ccccgtcggc
     2461 catatgctcg atgacggcca caaagcgccg gtaatcctgc gacaactcct cgatgcgctc
     2521 ccgctgctgc tcggtcatcg cgctgaaggc ccgcaccagg tcgccgatct cgtcccgccg
     2581 ctgggtcaac gcccgcgcgg tgccgtcgcc ggcggcgatg cgccgcgcca cctcggccac
     2641 gtcgttgagc ggccgcgcga tgcgcgacga caccagccac gccgccgcca atgccccgac
     2701 gaggcccacg gccacggccg cgaccagcgc attgcggacg caaacggcgt cgatcaaaca
     2761 ggcctcgcgc cgcagcagca ggaacagcac aagccccatc agcagcacgg gggcgatgag
     2821 cgggacgacg atttggcgaa acaggcgggg gatcatggca cgctcagccc tcgaagcggt
     2881 agccgatgcc gcgcacggtg accagccgcg ccgggttggc cgggtcgcgc tccagcttct
     2941 cgcgcagcca gcgcatgtgg acgtcgacgg tgcggctgtt gccgtcgaac gtccagcccc
     3001 agacgcgctc cagaatgagg tcgcgcgaca gggcgatgcc gcggttgcgg gccagaaaga
     3061 gcagcaggtc gaattcgcgc ggcttcaggc cgacgacttc gccgcccagc cgcacctcgc
     3121 gccgccccag gtcgagcacc agatcgccga agatgagcgc gccggtgtcg ggcggtcgcg
     3181 actcctgtcg ggtcgtcgcg ggcgcggcat cgcgcagctc ggccgccagc tcctcgcgga
     3241 tcagctccgc ccgccgcagc agcgccttca cacgggccat caattcgcgc atgctgaacg
     3301 gcttggtcag atagtcgtcg gcccccatct ccagcccgac gacgcggtcg acctcgtcgc
     3361 tgcgggcggt gagcatcagg atgggcaggt tggtctcctt gcgcagcagc cggcagacct
     3421 cgaagccgtc gatgccgggc agcatcacgt cgagcagcac cacatccggc tgctcgcgcc
     3481 gcgccgcctc cagcgccata aggccgttcc cggcctccac gacctcatac ccctgatggc
     3541 gcaggttata ggccaccgtc tcacgcaggg tggcgtcgtc ttcgactagc aggacacgat
     3601 agggcatgga ttcggtctct ttccgcgcgg ccgccgggat ggggcggcgg ccgattcggg
     3661 gccattgtaa cgccgccggg gtgcgggcac aacgttcaga attacgaatt acgaattatg
     3721 aattatgaat gaagagcgtc acttgccact cgtcactcgt ca
//
LOCUS       DAOMQG010000341         3753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10099_length_3753_cov_5.786101, whole genome shotgun sequence.
ACCESSION   DAOMQG010000341 DAOMQG010000000
VERSION     DAOMQG010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10099_length_3753_cov_5.786101"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLC06_14210"
     CDS             <1..848
                     /locus_tag="PLC06_14210"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HRO91648.1"
                     /translation="FMTEQTIGLLLVNHGSHSATWRQALLDLEARVTDSILAGGLIQG
                     VRTAHMEYTEPSIATQMKAFDEAGFTDVIIVPILLTVSSHSADDIPTILGQKHDPEVV
                     AELTSENIEIYTPAATIHFAPLLDFSEALQKNVLMRVRELSKQPENEGLALIGYGDAA
                     YDKEWSELFEKVAGHVKHHTGISYHSYGWCGHLVHYNPDKTTDAVNAVLGEKDAAIVI
                     PVLVAYDDMFQKNIIGRGIEKIEGHQERVLYAADAILPDPEIDEWVIRISGEFAGMIA
                     DRIAA"
     gene            1325..2242
                     /locus_tag="PLC06_14215"
     CDS             1325..2242
                     /locus_tag="PLC06_14215"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRO91649.1"
                     /translation="MTTSFTERLMRVAMPAIRLMQDHMPFSAANWFLRQSMKHLRLGD
                     GVTHEAVSAGGVPCIWLVPQDDPAGRVLLYLHGGGFVFGLTAQHMGLGAYLARKMASR
                     VLMVDYRLAPQHPFPAALDDCAAAYRWLLEQGVPAGNITVAGDSAGGNLTLTLLMKLR
                     DEGDPLPAAAACLSPVADLSRVNDQRAYRDPLLSPRAMERYSTAYAGDHDPRDPLISP
                     AFGRWHDLPPLLIHAGEDEILREDALQIEALARAAGVDARLEIYPRMWHVWQIYLAMP
                     EATQSLDDIAQFLRSHQHSSQTTPAATPA"
     gene            2246..3184
                     /locus_tag="PLC06_14220"
     CDS             2246..3184
                     /locus_tag="PLC06_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRO91650.1"
                     /translation="MTEPTTAVDAHPDRRRGTLSGAAAVLIWSTLAALTTLSGSIPPF
                     QLLAMSFTVAFLVGLALWVWQDHRSPPPAGGSIFRHFQLPAAVWALGIYGLFGNHFAY
                     FTAMRLAPPVEAGLLNYLWPLLIVVFAGFLPGERLHGRQIAGALLGFAGAVLIVTRGQ
                     GLYIDPRYATGYLFAVAAGVIWASYSVLSRRARNVPTSAVGAFCAATAVLALLCHLAL
                     EETVRPSAVEWLAVMGLGLGPVGTAFYVWDYGVKHGNIQTLGALSYSIPLMSTALLIA
                     LGLAEATWALAAAAVLITVGAALASWRGGREAGGTP"
     gene            3288..3698
                     /locus_tag="PLC06_14225"
     CDS             3288..3698
                     /locus_tag="PLC06_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HRO91651.1"
                     /translation="MRKILEKCPTCGGEFAVTELTCTTCSTVVRSAYSPCPFCRLSPE
                     DLEFMLLFVRNRGNVKDMERELGVSYWTIRGRLNEVITAMGLEPGGELEGEAPSPTRA
                     AAADPAAARQQVLDRLRRGELSAEEAAELLRIRN"
BASE COUNT          686 a         1182 c         1151 g          734 t
ORIGIN      
        1 gctttatgac cgaacaaaca attggattgc tgctggtgaa tcatggctcc cattcagcca
       61 catggcggca agccttgctc gatctggaag cgagagtgac cgattccatc ctcgcgggtg
      121 gcctcattca gggcgtgcga acggcccaca tggaatatac cgagccgtcc atcgcgacgc
      181 agatgaaggc gttcgacgag gcagggttca ccgacgtgat catcgtgccg atcctgctga
      241 ccgtcagctc ccattcggcc gacgacatcc ccaccatcct gggccaaaaa catgaccccg
      301 aggtcgtggc cgagctgacg agcgaaaaca tcgaaatcta tacccccgcg gccacaatcc
      361 attttgcgcc tctgctcgac ttttctgaag cgttgcagaa gaatgtgttg atgcgcgtcc
      421 gggagctgtc gaagcaacct gaaaacgaag ggctggcgct tatcggttac ggcgacgcgg
      481 cctatgataa ggaatggagc gaactgttcg agaaggtggc cgggcacgtg aagcaccata
      541 caggcatcag ctatcacagc tacggctggt gtggccacct ggtgcattac aaccccgaca
      601 agacgacgga cgcagtcaac gcggtgctgg gagagaagga cgccgccatc gtcatccccg
      661 tcctcgtggc ttatgacgat atgttccaga agaacatcat cggccgcggc atcgagaaga
      721 tcgaagggca tcaggagcgc gtcctctacg ccgccgacgc catcctgccc gaccccgaga
      781 tcgacgagtg ggtgatccgc atcagcggcg aatttgcggg catgatcgcg gacagaatcg
      841 cggcctgatt cgtgcctttt gggttccgga tttgatgacg agcaacgcct ggcgttgctc
      901 gttttgtatt tgatgccggg cggcaacgac cgatcctcat cccgtcaatc gcgcgggtcg
      961 acaaacatga gatcttccgg ccggtcagga catggtcgcc aatgtcggcg ggcgatctgg
     1021 ggaatgcgaa tgaacgcgaa tgaagaagga gaacaaccag gattttcatc atatccccat
     1081 tcgcgtccat tcgcgtccat tcgcggtctc cacagcagaa ggagaccact tggctaatta
     1141 ttgcgggtat ggccgtagga gtaggacagg gcggccgaac ggccttatct ttgcaaaacc
     1201 ccgtcgttgc cggttgtccc gtccctacat ctatatccgc aataatttgt taatatccaa
     1261 cagcgttgca ggctcccccg ctttatattt catggcaatc ataacattcg tataatagat
     1321 ccccatgaca acttccttca ccgaacgatt gatgcgggtc gccatgcccg ctatccgtct
     1381 catgcaggat cacatgccct tttcggcggc aaactggttc ctgcggcaaa gcatgaagca
     1441 cttgcggctg ggcgatggcg tgacgcacga ggcggtatcg gccggcggcg tgccctgcat
     1501 ctggcttgtc ccgcaggacg acccggccgg ccgcgtattg ctctatctcc acggcggcgg
     1561 cttcgttttc gggctgacgg cccaacatat ggggttgggg gcgtatctgg cgcggaaaat
     1621 ggcgtcacgc gtcctgatgg tcgactatcg cctcgcgcca cagcacccct tcccggcggc
     1681 gctcgacgac tgcgcggcgg cttatcgttg gctgctggag cagggcgttc cggcgggcaa
     1741 catcaccgtg gccggcgatt cggccggcgg caatctgacc ctcaccctgc tgatgaagct
     1801 gcgcgacgag ggcgatccgc tgcccgcggc cgccgcctgc ctctcgccgg tggccgattt
     1861 gtcacgggtc aacgatcaac gagcgtaccg ggacccgctg ctgtcgccca gggcgatgga
     1921 acgctactcg accgcctatg cgggcgacca tgacccccgc gaccccctga tttcgcccgc
     1981 cttcggccga tggcacgacc tgccgccgct gttgatccac gccggcgagg acgagatatt
     2041 gcgcgaggat gcgcttcaga tcgaagcatt ggcccgcgcg gccggggtcg atgcgcggct
     2101 ggaaatctac ccgcgtatgt ggcatgtctg gcagatctac ctggccatgc ccgaggcgac
     2161 gcagtcgctg gacgacatcg cccaattcct gcgatcccat cagcattcgt cgcaaacaac
     2221 cccggccgcg acccccgctt gacccatgac cgagccgaca accgcggttg acgctcaccc
     2281 cgatcgacga cgcggcacac tgtcgggcgc ggctgccgtc ctgatctggt ccacgctggc
     2341 cgcccttacg accctcagcg gcagcatccc ccccttccag ttgctggcga tgtcgttcac
     2401 cgtcgccttt ctggtggggc tggccttgtg ggtctggcag gatcatcgct cgccgccacc
     2461 cgccggcggg tcgatctttc ggcatttcca actaccggcg gcggtgtggg cgctgggcat
     2521 ctatggcttg ttcggcaatc attttgccta cttcaccgcc atgcgcctgg ccccgccggt
     2581 ggaggccggg ctgctcaact acctgtggcc gctgctcatc gtcgtctttg ccggattttt
     2641 gcccggcgag cggctgcacg gccgccagat tgccggggcc ttgctgggct ttgccggggc
     2701 ggtgctcatc gtcacccgcg ggcagggact ctatatcgac ccccgctatg ccaccggcta
     2761 tctgtttgcc gtggccgccg gggtcatctg ggccagctac tcggtgttgt cgcggcgggc
     2821 gcgcaacgtg cccaccagcg ccgtcggcgc gttctgcgcg gccacggccg tcctggcgct
     2881 gctgtgtcac ctcgcgctgg aagagacggt gcggccgtcg gccgtcgagt ggctggcggt
     2941 gatgggcctg gggctggggc cggtgggcac ggccttctac gtctgggact acggcgtcaa
     3001 gcatggcaac atccagacgc tgggcgcgct gtcctactcc atcccgctga tgtccacggc
     3061 gctgctgatc gcgctgggat tggccgaggc cacatgggcg ctggccgcgg cggcggtgct
     3121 catcactgtt ggggcagcgc tggcgtcgtg gcgcggcggc cgagaggcag ggggtacgcc
     3181 ctagaatttt ctgcgctcgc caaaatcttt ggtatcaata tcaaaaatct tgttgacaac
     3241 aacaaaataa tgagttatca tgcctaatag acaggagaaa ttgtcgtatg cgcaaaattc
     3301 ttgagaaatg ccctacctgc ggcggcgaat tcgccgtcac cgagctgacc tgcaccactt
     3361 gcagcaccgt ggtgcgcagc gcctacagcc cgtgcccttt ctgtcgcctg tcgcccgaag
     3421 acctggagtt catgctcctc ttcgtccgca atcgcggcaa cgtgaaggac atggagcgcg
     3481 agctgggcgt gtcgtactgg accatccgcg gccgtctcaa tgaggtcatc acggcaatgg
     3541 gattggagcc gggcggcgag cttgagggcg aggccccatc accaacacgc gccgccgcgg
     3601 ccgaccccgc agccgcccgc cagcaggttc tcgaccgctt gcgccgcggc gaactctcgg
     3661 ccgaggaagc ggcggaactg ttgagaatta ggaattagga attacgaatg gctttgttgc
     3721 atgaaaaagc gcaaagccaa aatgatagaa atg
//
LOCUS       DAOMQG010000342         3701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10263_length_3701_cov_9.565826, whole genome shotgun sequence.
ACCESSION   DAOMQG010000342 DAOMQG010000000
VERSION     DAOMQG010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10263_length_3701_cov_9.565826"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..807
                     /locus_tag="PLC06_14230"
     CDS             202..807
                     /locus_tag="PLC06_14230"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRO91652.1"
                     /translation="MITQPIIDSFETKTGIRVHVRPLMAEDAPYLVDLFEHMSSESRY
                     NRFMQAIDHIDIDRIWSEAEHIAHLIDGDSYGLVAFVDLPDRDHTPVGSARFVRFSPQ
                     QAEIAVSVRDDLQHMGIGTHLLQLLIETASEQGIEQLVGIARNSNEPMWAMLKKLGYR
                     LERQSEGSYSQIVLHISEVRGAANGWPDTAADYSPEPQLIG"
     gene            complement(781..2598)
                     /gene="sppA"
                     /locus_tag="PLC06_14235"
     CDS             complement(781..2598)
                     /gene="sppA"
                     /locus_tag="PLC06_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRO91653.1"
                     /translation="MPSKIMQALADGSAAAGITYRNGIRRLRNAEIDYVVLPVGGPLP
                     ERSSPKRGFLERRLPLPPDPLSMQELNSKLQIIADADNVRGVVFVFRGFSAGLATLQN
                     FRAAVLRLRAAGKEAIVYTPYVDLAHYYAASAAGAIIIPPSSHFEVLGLYTEVTFLKD
                     TLAKAGVKAEVVQISPYKTAMDRFSRDDFSPEYRAQLDWLLDDQFDMLTADMAAARGL
                     EQAAIRQIIDDGPLTPEAALALGLVDGVAYDDQLPSWLEKRTHAERSAIAAETATGQG
                     DAISDSTSPKGKDSTKPIRLMQWRQARRLLIEKHRRRPRRYIGVVSLEGLIDMGTSRT
                     PPIDLPIPFIGGKTAGDQTLVALLRRVEKLDDMAALIFHVDSGGGSALASDLIGRQIE
                     QIAARKPVVVYMGNVAASGGYYVSALARHIMSQQATLTGSIGVIQTRVNSDGLYGKIA
                     ANRVAVERGHHAGLYRSSGPMSAEDREINWRTINVIYDQFKAVVARGRNMTTEQVHEV
                     GAGRVWTGRQAQGHGLVDSHGDFVDAIRKAAELAALPTDDEYTIPVTNFIARDSSYTL
                     PGNAPKEAVETLSRLLTGEGLRELNGQPLMLLPYELRFG"
     gene            2746..3399
                     /locus_tag="PLC06_14240"
     CDS             2746..3399
                     /locus_tag="PLC06_14240"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRO91654.1"
                     /translation="MRPNAQFTLIFANGMLERTAWLAPYLAQARSVIAADGGLRHLLA
                     LGRLPDILIGDLDSLPEGAEAVLVGEDIDVIRFPRAKDETDLELALLHAIERFPDDVL
                     LIAGGFGGRIDHTLANITLLARPAFIGRAIYFIEEDQMAWLIDDETTITGRVGDVVSL
                     IPLGGDVAVIETSGLRWSLANETLSLGPARGMSNEMTAARAQIRIAGGLLLCVHTRR"
BASE COUNT          707 a         1038 c         1141 g          815 t
ORIGIN      
        1 attagcctgg tttgctacct atcaatacgc catattttgt gaaaggttgc atcgttttgg
       61 atggtcttct attgaatagc acagacaaga tgaagtttac cggtcgatcg ttaacgatct
      121 gtttgaatgg ccggggggtc ttgattttcg ttcgcggcta tgttaatcta aatatgacgc
      181 gatgcgtcat aagtgaaaac catgatcact caaccgataa tcgattcgtt tgaaacaaag
      241 accggtattc gtgttcatgt acggccgttg atggccgaag atgcgcccta tctggtcgat
      301 ctcttcgagc atatgagttc ggagagtcgt tataaccgat tcatgcaagc catcgaccat
      361 atcgacatcg atcggatatg gtccgaggcg gagcatatcg cgcacctgat tgacggggac
      421 agctatgggt tagtggcgtt cgtcgatttg cccgatcgcg accatacgcc ggtggggtcg
      481 gcccggttcg tgcggttttc gccgcagcag gccgagattg cggtctccgt gcgcgatgac
      541 ctgcaacaca tgggcatcgg tactcacctt ttacagttac tcatcgagac cgcttccgaa
      601 caagggatag agcagttggt cggcattgcc aggaacagca atgaaccgat gtgggccatg
      661 ctgaaaaagc tcggctaccg gctggaacgt cagtcggaag ggagctactc ccagatcgtg
      721 cttcatatta gcgaggtgcg cggtgctgcc aatggttggc cggacacggc ggccgactac
      781 tcacccgaac cgcagctcat agggtaacag catgagcggt tgaccgttta gctcgcgcag
      841 gccctcgccc gtcagcagac ggctgagtgt ctccactgcc tctttcgggg cgttgccggg
      901 aagcgtatag ctcgaatccc gggcgatgaa gttcgtcacc gggatcgtat attcatcatc
      961 agttggaagg gcggccagtt cggccgcctt tctgatcgcg tccacgaaat ccccatggct
     1021 gtcgacgagt ccgtgccctt gcgcctgtcg cccggtccag acccggcccg cgccgacctc
     1081 gtgcacctgt tccgtcgtca tgtttcggcc gcgcgcgacg actgccttga attgatcgta
     1141 gataacgttg atcgtccgcc agttgatttc ccggtcttcc gccgacatgg ggccggagct
     1201 gcgatacaac ccggcatggt ggccccgctc aacggctacc cggttggccg ctatcttgcc
     1261 atatagccca tccgagttga ccctggtctg gatgacgccg atcgatccgg tcaacgtcgc
     1321 ctgttggctc atgatgtggc gggccaatgc ggagacgtag taaccgccgg acgcggccac
     1381 gttgcccatg tagaccacaa cgggcttcct ggcggcgatt tgttcgattt gccggccgat
     1441 cagatcggag gccaacgccg aaccaccgcc ggaatcgaca tggaagatca gagccgccat
     1501 atcgtcgagc ttttccacac gtcgcaacaa ggcgaccagc gtctgatcgc cggccgtttt
     1561 gccgccgata aaagggatgg gcaaatcaat gggcggcgtg cggctggttc ccatgtcgat
     1621 tagcccttcg agggacacca cgccgatgta gcgtcgcggg cggcggcgat gtttctcaat
     1681 caggagccgg cgcgcttgcc gccattgcat cagtctgatc ggcttggtcg aatccttccc
     1741 cttggggctt gttgaatcag aaatggcgtc gccctggccg gtggccgtct ccgccgcgat
     1801 ggcggagcgc tcggcgtggg tgcgtttttc aagccatgaa ggtagttggt cgtcgtaggc
     1861 gacaccatcc accaggccca gggccagagc agcttctggc gtcagcggcc catcatcgat
     1921 gatttgccgt atcgccgcct gttccaggcc acgcgcggcg gccatgtcgg ccgtcagcat
     1981 atcgaactgg tcgtcgagca accagtccag ttgggcgcga tattccggcg aaaagtcatc
     2041 gcggctgaag cgatccatgg ctgtcttgta gggcgaaatc tggacgactt ccgctttcac
     2101 gccggccttg gccagcgtat ctttcagaaa ggtaacctcg gtgtaaaggc ccaaaacctc
     2161 gaaatgactg ctggggggaa tgatgatcgc gccggcggcc gaggcggcgt agtaatgggc
     2221 cagatcgacg tagggcgtgt agacaatggc ttccttgccc gcggcccgca ggcgcagcac
     2281 ggccgcgcgg aagttctgga gcgtggccag gccggccgaa aaaccgcgaa agacgaatac
     2341 cacgccgcga acattgtccg cgtcggcgat gatctggagt ttgctgttta actcctgcat
     2401 gctgagcggg tcgggcggca aggggagccg ccgctcgagg aagccccgtt tggggctgct
     2461 gcgttcgggc agcgggccgc cgacgggcag gacgacatag tcaatttctg catttcgtag
     2521 tcgccgtatt ccattgcgat atgtgatgcc ggcggcggcc gatccatcgg ccaaggcctg
     2581 cataatcttc gatggcatct ggcttaactt ccggcgagag gccggcggtt ggacagggag
     2641 atttggcgtt tcaatcattc tttttgaatc cgggatgtta tcgttacttg catgagttta
     2701 gttcgtaggt catggtgggg tcaagtgaga tagaatagct cacatatgag gcccaacgca
     2761 caattcaccc tgatcttcgc caatggcatg cttgagcgca cagcctggtt ggcgccttac
     2821 ctggcacagg ctcgctcggt gattgccgct gatggcggct tgcgccactt gctggcgctg
     2881 ggtcgcctgc ccgatattct catcggcgat ctcgactcgc tgcccgaagg tgcggaagct
     2941 gtgctcgtcg gcgaggatat cgacgtcata cgcttcccgc gagccaagga tgagactgat
     3001 cttgagctgg ccctgctgca cgccatcgaa cgcttccccg atgatgtttt gttgatcgcc
     3061 ggtgggttcg gcggccggat cgaccatacc ctggccaata tcacgcttct cgcgcgtccc
     3121 gccttcatcg gccgcgccat ctattttatt gaggaagatc agatggcgtg gctcatcgac
     3181 gacgaaacga cgatcaccgg ccgggttgga gatgtcgtct cgttgatccc tctggggggc
     3241 gatgttgccg taattgaaac aagcggcctt cgctggtcat tggcgaatga gaccttgtcg
     3301 cttggccccg cgcgggggat gagcaacgag atgacggccg ctcgcgcgca aattcgcatc
     3361 gccggtggac tgttattgtg cgtgcacacc cgccgatgaa agacctgaca ggtgggcgcg
     3421 tggagttgtc ggtttgacgg gagcgttcgg tgcgcccacc tgtcaggtcc gggttaggtc
     3481 aggtgaatta cgaattacga accgatgaaa gacctgacag gtgggcgcgt ggagttgtcg
     3541 gtttgacggg ggcgttcggt gcgcccacct gtcaggtctg ggtgcggtca ataatcccaa
     3601 ttattggtat acttcgctcc gccggtggca gattacccgg ctggataaaa ctgcggcggt
     3661 cgatgcaccc gcctcaacaa aacaaggaga accaaatgac c
//
LOCUS       DAOMQG010000343         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10296_length_3692_cov_9.742095, whole genome shotgun sequence.
ACCESSION   DAOMQG010000343 DAOMQG010000000
VERSION     DAOMQG010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10296_length_3692_cov_9.742095"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1253
                     /gene="cyoE"
                     /locus_tag="PLC06_14245"
     CDS             318..1253
                     /gene="cyoE"
                     /locus_tag="PLC06_14245"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRO91655.1"
                     /translation="MSLSKMTDSPDSPLESGAAAISGRRSALRTAVVLFKLRIVFLLL
                     VAATGGAFLAAGGWPGWGVMALVWLAGGMAAAGASALNQYWERGKDGLMGRTRKRPLV
                     NGDITNPRWVPYVGVALILVPVLAVLPFNRPLAFFLALGAFIYVGIYTIWLKPRTLLN
                     IVIGGAAGSAAVLSGSAAAGMWDAPGALALAAILFLWTPFHFWSLAILYREEYSRADV
                     PMLPTQTTPRRAAWWVMSHTLPTGILSLLMVVLPGLGWIFFVPVLIITADLFYRNVQL
                     IRDPSPVNARGLFMSSNYYLLVLLVAICVDLMLPW"
     gene            1661..2794
                     /locus_tag="PLC06_14250"
     CDS             1661..2794
                     /locus_tag="PLC06_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRO91656.1"
                     /translation="MKARLSLFLVLLTVLALALAACGGGGQQAAPTEAPAATEAAPAA
                     TEAPAAAATTAPAVPTGNTLEAVRTRGTLKCAGNQSVPGFGYINPDTNEFEGFDIDFC
                     KVFAVAALGDATKVEVRPTTANERFPVLQTGEVDVLSRNTTWTISRDTSLGLNFAPVT
                     FYDGQGMMVRVDSGIDTLEGLAGGTICVQSGTTTEKNLADVYRALGVEFTPVVFDDAD
                     ATREAYDQGQCDGFTTDKSGLVSQQILLADPAAHKILDATMSKEPLAPAVRHGDDNWF
                     DIVKWSVFCTIAAEEYGITSANVDSFLGGEDPVIQNLMGETGDLGVAMGVNNDFCYQI
                     VKQVGNYGEIYDRNLGPDTPFNLPRGLNALYTQGGIQYAPPFR"
     gene            2904..>3692
                     /locus_tag="PLC06_14255"
     CDS             2904..>3692
                     /locus_tag="PLC06_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRO91657.1"
                     /translation="MTELTPQAPKGTESVPFWRDIRVLGVLAQIAFLIVVITGFSWVV
                     RNVTQNMSTLGGSQFLCADGTSSFRCAFDFLRLDASFAISESLIPYDPSDSYARAMLV
                     GALNTIKVSILGIILATVLGTVTGIARLSSNWLVSSVARWYVDLIRNTPLLLQLFFLF
                     FAVILAFPPIKDAIQPFGLPVYLSQRGIDLPWPVLMSSFTTWLIFLVAGAVLGAAVWW
                     WLGRRERETERDVNRSLWFVVTFLAVAIAGWFIATTMAAGNQGFL"
BASE COUNT          640 a         1068 c         1135 g          849 t
ORIGIN      
        1 tgggaactgc acccatgatg ttttttcctc aattttagat ttaacgccgc gggcgggaga
       61 cgaccggtat cttctgtcta acagaatctg tgatcaccac acgcctctat tggcgataga
      121 cgacaaaaaa caagcggcga aaagccgcga atcaggatta tagtcgcacc tcggcggcgg
      181 gtcaagacga gatcggccct ccttgggcat tttcaggctt gggcgcgccg atcgtttgtc
      241 cctccttatg gggcttgcta aaatcctccg cgttgtcgga aacacagggt gtggggatag
      301 cctggttggg agatgatatg tcgttatcga agatgacgga ttcgcctgat agcccgctgg
      361 aaagcggcgc ggcggcgatt agtggccggc ggagcgctct gcggacggcg gtcgtgctgt
      421 tcaaattgcg catcgtcttt ttgttgctcg ttgccgccac cggcggcgca ttcctggcgg
      481 ccggcggctg gcccgggtgg ggcgtgatgg cgctcgtctg gctggcgggg ggcatggccg
      541 ccgcgggcgc gtcggcgctg aaccagtatt gggagcgcgg caaggacggg ctgatgggtc
      601 gcacgcgaaa gcgtccgctt gtgaatggcg acatcaccaa cccgcgttgg gtgccctatg
      661 tgggtgtcgc gttgatcctt gtcccggtgc tggcggtgct gccgtttaat cgcccgctgg
      721 cgttctttct ggcgctcggc gctttcatct atgtggggat ctatactatc tggctgaagc
      781 cgcggacgtt gctcaacatc gtcatcggcg gcgcggccgg cagcgccgcc gtcctaagcg
      841 gcagcgcggc ggccggcatg tgggacgcgc cgggcgcgct ggcgctggca gcgatcctgt
      901 ttctgtggac gccgttccac ttctggagcc tggccatcct ctatcgcgag gaatacagtc
      961 gagccgacgt gcccatgctg ccgacgcaaa caacgccacg aagggccgcc tggtgggtca
     1021 tgtcccatac gctgcctaca ggcatcctga gtttgctgat ggtggtattg cccggtttgg
     1081 gctggatatt cttcgtgccg gtgctgatca tcaccgccga tctcttttat cgcaacgtgc
     1141 agttgattcg cgatccgtca ccggtcaatg cccgcgggtt gtttatgtcg tcaaattact
     1201 acctgctggt cttgttggtg gctatttgcg ttgacctgat gctgccctgg taaacatcgt
     1261 ttcttcgccg aaaacgccct tcgccacctg attttccaga tcgggcggct ttttttgcca
     1321 aattgctaat cttgccctaa gattgtcgcc caactaccgg tgtgtgggct gattggatgc
     1381 ccgacaacgg ttgggggatg ggttttactg gtggcatggc tgacgggaaa gccgccatcc
     1441 cctttctgga agcaacttcg cgtggatcgc catccgcatc gaggaaggcg agagacggtt
     1501 ctcgccgtcg tgtcgcagtt gaatctgtcg gaaaccggcg tgtttccgac aggggagggc
     1561 aaacgggata acagaacatt tataaccgaa cacgaggaac gaattcagtg aaagaatccg
     1621 ataaaggatc ctatttaaag tttcggagga gaaaatagag atgaaagcgc gactgagttt
     1681 atttttggtt ctattgacgg tgctcgctct ggctctggcg gcgtgcggcg gtggtgggca
     1741 acaggctgcc ccgaccgaag ctccggctgc taccgaagcg gctcccgctg cgaccgaggc
     1801 cccggccgcg gccgccacga cggccccggc tgtccccacc gggaatacac tggaagctgt
     1861 ccgcacgcgc ggcacgctca agtgcgcggg caatcagtcc gtccccggtt tcggttacat
     1921 caaccccgac accaatgaat ttgaaggctt cgatattgac ttctgcaaag tcttcgccgt
     1981 tgctgcgttg ggcgatgcca ccaaggtgga agttcggccg accacggcta acgagcgctt
     2041 ccccgttctg cagacgggcg aggtcgatgt cctcagccgt aacacgacct ggacaatcag
     2101 ccgcgatacg tcgctgggcc tcaatttcgc tcctgtcacg ttctacgacg gtcagggcat
     2161 gatggtccgc gtcgatagcg gcattgacac gctggaaggt ctggcgggcg gcacgatctg
     2221 cgtccagtcc ggcaccacca ccgagaagaa cctggccgac gtctatcgcg cgctcggcgt
     2281 tgagttcacc ccggtcgtct tcgatgacgc cgatgccacc cgcgaggctt atgatcaagg
     2341 ccagtgcgac ggcttcacga ccgacaaatc cggcctcgtt tcgcagcaga ttcttctggc
     2401 tgacccggcg gctcacaaga tcctcgacgc gaccatgtcg aaggaaccgc ttgctccggc
     2461 cgttcgtcac ggcgacgata actggttcga catcgtcaag tggtcggtct tctgcaccat
     2521 cgccgctgaa gaatacggca tcacctcggc taacgtcgac agcttcctgg gcggcgaaga
     2581 tccagtcatc cagaatctga tgggcgagac gggcgacctg ggtgtggcca tgggcgtcaa
     2641 caacgacttc tgctatcaga tcgtcaagca ggtcggcaat tacggcgaga tctatgaccg
     2701 caatctgggt cccgatacgc cgttcaacct gccgcgcggc ctgaacgcgc tgtataccca
     2761 gggcggtatc cagtacgcgc cgccgttccg ttagtctttt ttgtaggggc gggtttgaga
     2821 cccgccccta ctgtttggca atatcgggca agggcgggta cgaagatcaa tacccgcccc
     2881 tgcaatagac aggaggcgcg ctcatgactg aacttacacc gcaggcgccc aagggcacgg
     2941 agtcggtgcc attctggcgg gatattcgcg tgttgggtgt gctggcccaa attgcgtttc
     3001 tcatcgtcgt catcacgggg ttttcctggg tggtgcgcaa cgtcacgcag aacatgtcga
     3061 ctcttggcgg cagccagttt ctctgcgccg acggcacgag cagctttcgt tgtgcgttcg
     3121 atttcctgcg tcttgatgca tcattcgcga tctctgagtc gctcatcccc tatgatccca
     3181 gcgactccta cgcccgcgcc atgctggtcg gcgcgctgaa tacgatcaag gtttcaattc
     3241 tgggcattat tttggcgacg gtgttgggta cggtgacggg tatcgcccgg ctttcctcta
     3301 actggcttgt cagttccgtg gcgcggtggt atgtcgatct cattcgcaac acgccgctgt
     3361 tgctccagct tttcttcctg ttcttcgccg tcattttagc ctttccgcct atcaaggatg
     3421 ccatccagcc cttcggcctg cccgtttatt tgagtcagcg tggcattgat ctgccctggc
     3481 cggtcctcat gtcgtccttc acgacgtggt tgatatttct cgtggcgggg gccgtgttgg
     3541 gcgcggccgt ctggtggtgg ctcggccgcc gcgagcgcga aacggagcga gacgtcaatc
     3601 gctcgctgtg gtttgtggtg acgtttctgg ccgtggcaat cgccggatgg tttattgcca
     3661 caaccatggc cgccggcaat cagggatttc tg
//
LOCUS       DAOMQG010000344         3677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10358_length_3677_cov_8.932082, whole genome shotgun sequence.
ACCESSION   DAOMQG010000344 DAOMQG010000000
VERSION     DAOMQG010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10358_length_3677_cov_8.932082"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2277
                     /locus_tag="PLC06_14260"
     CDS             <1..2277
                     /locus_tag="PLC06_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRO91658.1"
                     /translation="HMSSRGRSLLVGGLALLLAVALFAWRPSSSASLYSGYARADNGI
                     LSLEVTVNPPAGSPGTKMQMVARVTNHAGQPLTPSIVLRLPPGVVADMYALPSGATFN
                     LQQNQIDWLPVVPAAGTVEFTADMVVQTANVTEPEQFVEALMHHQGDLREALAPVWIG
                     IAPLVGAVTAQGQVSVGQPVRLHADIAGPGPITTIWDLGDGRRLELSDPEIVFPSAGN
                     HDIVVEVSNPAGRVTKHFTLTVLPQPVAAFSPDDDTPAIGQAVTFRSLSGGQPPLKVF
                     WDFGDGATVMGEQQPVHAYNQGGVYRVRLAIENDFGRSEAVWDITVGGAPVADMIIGD
                     RTAVGLSFTGQAFGDDTVARYLWDMGDGRQYDGAAVSHVYRFPGDYYVTMTADNGFDQ
                     TRVGRWVHVDPGTTSLFLPLAAHIGDSTTPTLAAGMVSAELAPPVESISETFTLEPIQ
                     FPAGTSPAEQLLAYLNAARARFGLPPMSYAYELSAAAQAHVLDKARFPDNPHVGTDGT
                     TAAERLLRSGYRGGYAGEATAWGFSDPREAVEFWVNSDSHRPLILNQLGTEVGIGYVE
                     DFSTTNIWHWTTEFGIAYGAPVKPVLRSLVPAAGYGALNTDVINYSWMWPVPLAPGER
                     FTVYLESGNRRIPLGSVAQPVYGSRYILSMDARGALGTMVNGAINLPGYNWVVRLEDG
                     LGNTLAEGERRLIAFSADPSIPVATAVPTIAIVTATPAALAPTQTPTLMPTAEPPTNE
                     PPPIIVTATPQPTPSSKP"
     gene            2374..3519
                     /locus_tag="PLC06_14265"
     CDS             2374..3519
                     /locus_tag="PLC06_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRO91659.1"
                     /translation="MGDDQIVLNGRYRIIDRIGSGGMSVVYRAQDLSLGRNVAVKVLH
                     ENLTDDEGFLRRFQREAHAAANLSHPNVVTVHDIGQDGNRHYIVMEFVDGRTLKQLVK
                     LQNQQGRTLSIEQALDLGIQICAGIGYAHRANLVHCDVKSQNILVTNDDRVKVADFGI
                     ARAISEATQHTVDSQIWGTPHYFSPEQAAGLGATPASDVYAIGVVLFELLTGRLPFLA
                     ETHTALALKHMHEPPPRASDINPLIPSQLDQILLKVLSKEPAARYRTGDQLGRILSAY
                     RESSLQTTGPISLLDVDVPSYEQKTAAYAYSYGSEPATTGDGDIIIHSGETRPHQRPP
                     SYPSSSIRTAVPPPERDWLAIGLGILAVAALLGLIPLWYFVFLAYSG"
BASE COUNT          697 a         1096 c         1084 g          800 t
ORIGIN      
        1 cacatgagtt cccgaggtcg ctcgctattg gttggtggat tggcgctgct gttggcagtt
       61 gcgctttttg cgtggcggcc gtcttcgtca gcttctctct actcgggcta tgcgcgcgcc
      121 gataacggca tattgtctct ggaagtcacc gtcaatcccc cggccggatc gcccggaacg
      181 aaaatgcaga tggtcgctcg ggtcacgaac cacgccgggc aacccctgac gccatcgatc
      241 gtgttgcgtc tcccgccggg cgttgtggct gatatgtatg ccttgccgtc gggtgcgacg
      301 ttcaatcttc aacaaaatca gattgattgg ttacccgtcg ttcccgctgc cggtaccgtg
      361 gaattcaccg ccgacatggt cgttcaaacg gctaacgtga cggaaccgga gcaattcgtc
      421 gaggccctga tgcaccatca gggtgacctg cgtgaggcat tggctccagt ctggattgga
      481 attgcgcctc tggttggcgc ggtgacggcc caggggcagg tttcggtggg tcagccggtt
      541 cgtctccatg ccgacattgc cggcccgggg ccgattacca cgatctggga tttaggagat
      601 ggccggcgac ttgaattaag cgatcccgag atcgtatttc cgtccgccgg aaatcacgac
      661 attgtcgttg aagtatcgaa ccccgcgggt cgagtcacca agcatttcac attgactgta
      721 ttaccccagc cggtcgccgc attcagcccg gatgatgaca cgccggccat tggtcaggcc
      781 gtcacgttca ggagcctcag tggtggccag ccgccgctga aagtcttctg ggatttcggc
      841 gacggagcca cagtcatggg cgagcaacag ccggtccatg cttacaacca gggtggggtc
      901 tatcgagtac gcctcgcgat tgagaatgat ttcggccgat cggaagccgt gtgggatata
      961 accgtcggtg gtgctcctgt ggccgatatg attatcggcg atcgcacggc agtgggcttg
     1021 tcattcaccg gtcaggcgtt tggggatgat acggtcgctc gttacctgtg ggatatgggc
     1081 gacggccgtc aatacgatgg ggccgcggtc agtcatgttt atcgttttcc cggcgattat
     1141 tatgtgacca tgaccgccga caacgggttt gaccaaaccc gggtcgggcg atgggttcat
     1201 gtcgatcccg gcacgacatc gctctttctg ccgttggcgg cgcacatagg cgattcaacg
     1261 acgccgaccc ttgccgccgg tatggtctcg gcggaactcg caccacctgt tgagtcgata
     1321 tccgagacct tcacgcttga gccgatccag ttccctgcgg ggacctcccc cgccgagcaa
     1381 cttctggcct atctgaacgc ggcccgggcg cggtttggtc tgccgccgat gagttatgcc
     1441 tatgagctta gcgcggccgc tcaggctcac gtgctcgaca aggcccgctt ccccgataac
     1501 ccccatgtgg gaactgacgg gaccacggcg gccgagcgct tgctgcgcag cggctatcga
     1561 ggcggctacg ccggtgaagc tacggcgtgg gggttctcgg atccgcgtga agctgttgag
     1621 ttttgggtca atagcgattc tcaccgcccc ttgatcctta accaattagg caccgaggtg
     1681 ggtatcggct atgtggagga cttctccacg acgaatattt ggcactggac gaccgagttc
     1741 ggcattgcct atggcgcgcc ggtcaagccg gtgttgcgtt cgctcgtccc ggccgccggc
     1801 tacggtgcgc tcaataccga tgtgatcaac tacagctgga tgtggccggt gccgttggca
     1861 ccgggtgaac gattcacggt atatctggag tccgggaatc gccggatccc gttggggagc
     1921 gttgcccaac cggtatatgg cagccgctat attctgtcga tggatgcgcg cggcgcgctg
     1981 ggcactatgg tcaatggagc gatcaatttg cccggctata attgggtcgt gcgccttgag
     2041 gatggtctcg gcaataccct cgctgagggt gaacggcggt tgattgcctt ctcggccgat
     2101 ccgtcaatcc cggtcgcgac ggccgtgccg acgatagcga ttgtcaccgc gacaccggcc
     2161 gcactcgcgc cgacccagac gccaactctg atgccgacgg ccgaaccgcc cacgaacgag
     2221 ccgccgccga tcatcgtcac ggcgacaccg cagcccactc cctcatccaa accctgatac
     2281 atcgcccgaa atcagggggc gtaaccgtca atatgatacg acgacaagcg ccccttgcga
     2341 cggagccgtc tccgttctac aattgaactt gatatgggag atgatcagat cgtcttaaat
     2401 gggcgctatc gcatcataga ccgcataggc agcggcggca tgtcggttgt ctatcgagcg
     2461 caggacttgt cattggggcg caatgtcgcc gtgaaggtgc ttcatgagaa cctgacggat
     2521 gacgaaggct tcttgcgccg gttccaacgc gaagcgcacg cggccgccaa cctctctcac
     2581 cccaatgtcg tcactgtgca tgatatcggc caggatggca accgccacta tatcgtgatg
     2641 gagtttgtcg acggccgcac gctgaagcaa ctcgtcaaac tccagaatca gcaggggcgc
     2701 acgctcagta tcgagcaagc gcttgatctg gggattcaga tatgtgcagg tatcggctat
     2761 gcccatcgcg ctaacctggt tcattgtgat gtaaagtccc aaaacatcct tgtgaccaat
     2821 gacgatcggg tgaaggtcgc cgatttcggc atcgccagag ctatcagcga ggcgactcag
     2881 cacacggtcg acagccagat ctggggcacg ccccattact tttcgccgga gcaagcggcc
     2941 ggtctgggcg cgacgcctgc ttccgacgtc tatgcaatcg gggttgttct attcgaattg
     3001 ctcaccggtc gattgccgtt cctggccgag acccacaccg cgttggctct caagcacatg
     3061 catgagccac caccgcgagc gtccgatatt aatccgttga tcccctccca acttgatcag
     3121 attctgctca aggttttgtc aaaagagcct gccgcgcgtt atcgcacagg cgatcaattg
     3181 ggccgaattc tgagcgccta tcgggaaagc agcttgcaga ctaccggccc aatctccttg
     3241 ctggatgtcg acgtgccgtc atatgagcaa aaaacggccg cctatgccta ttcatacgga
     3301 agtgaaccgg caacgacagg cgatggcgac atcatcattc attccggcga gacccgtccc
     3361 caccagcggc cgccttccta tccttcttcc agtattagga ctgccgtccc gcccccggag
     3421 cgggattggc tggctattgg cctgggcatc ctggccgtgg cggctctgtt ggggcttatc
     3481 cctctatggt actttgtatt cctcgcctat agcggctaaa atatcaggca ggatacccgg
     3541 ccttgggcca gaggcatgac catgaattca accagactga caagatggct cgtctatctc
     3601 ctgattggag ctgccgtagc ggccgtcttg tggagctata gctcggctcc ggcagcccaa
     3661 accgatatcc ctatcag
//
LOCUS       DAOMQG010000345         3676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10363_length_3676_cov_11.571941, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000345 DAOMQG010000000
VERSION     DAOMQG010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10363_length_3676_cov_11.571941"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1561
                     /locus_tag="PLC06_14270"
     CDS             287..1561
                     /locus_tag="PLC06_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO91660.1"
                     /translation="MDIPKPSPELHDYFNHHLSDYLDLLRGMVLINSFTANPAGVDAL
                     GRLTAERFASLGFTAETIASTDARYGRHLILTREGRSAGDTAAPVIGLISHLDTVFPA
                     AEEQANDFTFRIEGDRIYGPGTVDIKGGTVMICMVLEGLRLFHPEVFAGVTWRILLNA
                     AEEVLSRDFGLLCRHVLGPNALAALVFEQGDVRDGRYLLVTSRKGMARYHIQVEGRGA
                     HAGALHKRGANAIVQLADVVGQIAGFTDHERDLTFNVGTITGGTVTNRVPHLATADVE
                     MRAFDPEVFESGVARMMVLNGYCSVRSLDDDYPCRVEVDLEGRWTPWPSNEGTSYLVD
                     VWRAAAAENGMGDVTPQARGGLSDGNWLWDLLPTIDGLGPQGANAHSSERSADGSKDQ
                     EYALASSFVPKAMLNVAAIVRLVTMDGAGTHD"
     gene            1569..2723
                     /locus_tag="PLC06_14275"
     CDS             1569..2723
                     /locus_tag="PLC06_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRO91661.1"
                     /translation="MEWETLLNDCVEFAQRLIRTPSMPGEEQAIAALIAEELARLGYD
                     EVWDDPAGNIFGRVYGQDRSLPALALNTHLDHVDPGDPMLWPTPPFAAEIRDGRLIGR
                     GACDIKGPLAVQVYSAAGLLRAGERPRRDVVFCGVTQEEVGGAGALYWAQHLDYPVEL
                     VILGEPSSNELSVGHRGIVQFWLTFHGRSAHASAPERAVNPNYALASFLSQLRAAAAE
                     QLRVHPVLGPTSVSPTIIEVDTTSTNVTPAWTRVLLDFRTSSESVNSLRAFIDRLADD
                     FPHSLEYAEGMEPSPLDDSDEMIAGFYTPPDSQLVLRTRAALGRGMGREPELIRYRFA
                     TDGRHMAPYGIPVLGFAPGEEEMAHTAGESISLAQMAESLRGHMQLLREF"
     gene            2856..3563
                     /locus_tag="PLC06_14280"
     CDS             2856..3563
                     /locus_tag="PLC06_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRO91662.1"
                     /translation="MPNIDKLDRGQSQQRDGLTHQAIEDYVKTIYSLALEESPVSTSR
                     IAIARSVKPASATSMIQRLARLHLVNYEKHYGVTLTESGEKLALEVIRHHRLLELYLM
                     EALGFEWHEVHEQADILEHVISEKLEERIAAVLNHPEFDPHGDPIPALDGTMAVIDTT
                     ALSDLIVGEQARVARVPDDSNSELLRYLAGLELVPGAMVTIIEVAPFLGPLTIEVGDS
                     QKIIGRNLADLVLVQRM"
BASE COUNT          687 a         1160 c         1136 g          693 t
ORIGIN      
        1 gtcacggccg tcccatcggg catatatcgc atttccgggt cccggccaag acgccctaca
       61 atcacaagct tctgatacat ttgcgattct ccttaagttg tgccttgcgc tattcggccg
      121 agttgtcctc tgagccgcca aggcgaatag taacatgttc tacgcaaacg ttccatttga
      181 tttgatgcgt atctgcaaca taatatagca caaatgttca taaaaatcaa gacttccaga
      241 cgacaatttt ctggttgacc ctccctctcg gtgctataaa taggctatgg atatccccaa
      301 gccttccccc gaactgcacg attatttcaa ccatcatctc agtgactatc tcgacctttt
      361 gcgtgggatg gtgctcatca acagtttcac cgcaaacccc gccggcgtcg acgcactcgg
      421 ccgtctgaca gccgagcggt tcgcctcgct gggcttcacg gctgagacca ttgcttcgac
      481 cgacgcccgc tatggccggc atctgatcct cacccgcgag ggtcgttccg ccggtgacac
      541 ggccgcgccg gtcatcggac tcatctccca cctggatacc gtctttccgg cggccgaaga
      601 gcaggccaac gacttcacct ttcgtatcga aggcgaccgc atctatggcc ccggcacggt
      661 agacatcaag ggcggcacgg tgatgatttg catggtcctt gaagggctgc gcctcttcca
      721 ccccgaggtc ttcgcgggcg tcacatggcg catcctgctc aatgccgccg aggaggtgct
      781 gtcgcgcgat ttcggcctgc tgtgccggca tgtgctcggc ccgaatgcgc tggcggcgct
      841 ggtgtttgaa cagggcgacg tgagagacgg ccgctatctg ctcgtgacgt cgcgcaaggg
      901 gatggcgcgc taccacattc aggtagaagg gcgcggtgcc catgccgggg cgctgcacaa
      961 gagaggggcc aacgccatcg ttcaattggc cgacgtcgtc ggtcagatcg ccggcttcac
     1021 cgaccacgag cgcgatctga ccttcaacgt cggcacgatc acgggcggca cggtgaccaa
     1081 tcgcgtgccc catctggcca cggccgacgt cgagatgcgc gccttcgacc ccgaggtatt
     1141 tgagagcggc gtggcccgca tgatggtcct caatgggtat tgcagcgtgc gcagcctcga
     1201 cgacgactac ccttgccgcg tggaggtcga tcttgagggg cgatggacgc cctggccatc
     1261 gaacgagggc acgagctatc tggtggatgt gtggcgggcg gccgcggctg aaaacggcat
     1321 gggggacgtg accccgcagg ctcgtggcgg cctgagcgac ggcaactggc tgtgggatct
     1381 gctgccgaca atcgacggtc tcggcccaca gggggcaaac gcccattcat ccgagcgcag
     1441 cgccgacggc agcaaggatc aggaatacgc gctggcatcg tcgtttgtgc ccaaggcgat
     1501 gctgaatgtg gccgccatcg tccgccttgt gacgatggac ggggcgggga cgcacgatta
     1561 aggtgactat ggaatgggaa acattgctga acgattgcgt ggaattcgcc caacggctga
     1621 ttcgcacacc gagcatgccc ggagaggagc aagccatcgc cgcgctcatc gccgaagagc
     1681 tggcacgtct cggctacgac gaagtatggg atgatccggc cggcaacatt ttcgggcgcg
     1741 tctatgggca ggatcgcagc ctgcccgcgc tggcactgaa cacccacctc gaccacgtcg
     1801 atcctggcga cccgatgcta tggcccacgc ctcccttcgc ggccgaaatt cgcgacggcc
     1861 gcctgatcgg ccggggggcg tgtgacatca aggggccgct ggccgtgcag gtctacagcg
     1921 cggcaggact gctgcgcgcc ggtgaacggc cgcggcgcga cgtcgttttc tgtggcgtga
     1981 cccaggaaga ggtcggtggc gcgggggcgc tctattgggc gcagcatctt gattatcccg
     2041 tggagctggt catcctcggc gagccgtcgt ccaacgagct gtcggtgggc catcgcggca
     2101 tcgtccagtt ttggctgacg ttccacggcc gctcggccca tgccagcgcg ccggagcggg
     2161 ccgtgaaccc caactatgct ctcgcctcgt ttttgtcgca gctgcgagcg gccgcggccg
     2221 agcagctacg cgtccatccc gtcctcggcc ccacgagtgt ttcgccgacg atcatcgaag
     2281 ttgacacgac cagcaccaac gtcaccccgg cctggacgcg ggtgctgctc gatttccgca
     2341 cctcgtcgga gagcgtcaac agcttgcgcg cgttcatcga tcgactggca gacgattttc
     2401 cccattcgct ggaatatgcc gaaggtatgg aaccatcgcc gctggacgat tccgacgaga
     2461 tgatcgccgg gttctacacg ccgcccgata gccaattggt gctgcggacg cgcgcggcgc
     2521 tgggccgggg gatgggtcgc gagccggagc tgattcgcta ccgcttcgcc accgacggcc
     2581 gccacatggc cccctacggc atccctgtcc tcggctttgc gcccggtgag gaagagatgg
     2641 cccacaccgc cggggagagc atcagcctcg cccagatggc ggagagcttg cgggggcaca
     2701 tgcagctgtt gcgggagttc tgaggtgtga tcgccgacag gcgacaggcg accggaaatg
     2761 ttcatggatt atggcctgtt gatgggcgat gagtggcggt caaaagccgc ttgacgccgt
     2821 ccccgctgag ggttacccta tggatggaga acgctatgcc caatatcgac aagctcgacc
     2881 gcggccaatc ccaacagcgt gatggcctca cccaccaggc aattgaggat tatgtcaaga
     2941 cgatctactc gctggcgctg gaggaaagcc cggtatccac atcccgcatt gccatagccc
     3001 gctcggtgaa acccgcctcg gccaccagca tgatccagcg gctggcccgg ctgcatctgg
     3061 tcaactacga gaagcattac ggcgtaacct tgaccgagtc cggcgaaaag ctggcgctgg
     3121 aggtcatccg ccatcatcgc ttgctggagc tttacctcat ggaggcgctc ggctttgaat
     3181 ggcatgaggt tcacgagcag gccgacattc tggagcatgt catcagcgag aagctggaag
     3241 agcgcatcgc cgctgtgctg aaccatcccg aattcgaccc ccacggtgac ccaatccccg
     3301 cgctggacgg gacgatggcg gtgatcgaca ccaccgcgct ctccgatttg atcgtgggcg
     3361 aacaggcgcg ggtggctcgc gtccccgacg actccaacag cgagttgttg cgctatctgg
     3421 ccgggctgga actggttccg ggagcgatgg tcacgatcat cgaggtggct ccgttcctgg
     3481 ggccgctgac gatcgaggtg ggcgacagcc agaagatcat cggccgcaac ctggccgacc
     3541 tcgtccttgt ccagcgaatg taaaagccag gcgacagccc tactgacccg tggatcagta
     3601 ttcagtagat cagtattcag ttactgaccc actgacccac tgacccactg acccactgac
     3661 ccactgaccc actgac
//
LOCUS       DAOMQG010000346         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10381_length_3670_cov_8.069156, whole genome shotgun sequence.
ACCESSION   DAOMQG010000346 DAOMQG010000000
VERSION     DAOMQG010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_3670_cov_8.069156"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PLC06_14285"
     CDS             <1..1458
                     /locus_tag="PLC06_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRO91663.1"
                     /translation="SMFSSQVPHEIERYEIPADLHTALALLAQHGKAARIIAGGSDLL
                     LELERGQRKGVTTLIDITRVPGLSAISRDEEGLIHLGALVTHNQVIHSRHIIDHAFPL
                     AQACIEVASPQLRNRGTVAGNLVTASPANDTISALRALNAQVILASTHGWRMVDLREF
                     YTGVRKTVMTPDEMVVDIVIPPMPPTARGIFVKLGLRRAQAISVAHLTAVLDFADDGR
                     VNRARIALGSVAPTIINAEAAESYLLDKEMTDDELTVAAALVASAAHPIDDVRGPASY
                     RAEMIEVMARRALVALRDDRQRETWPADPVMLWGATDGIFPTGERYAASFGPDTPIES
                     TVNGRAVSAPGMEKTLLRWLREEGLLTGTKEGCAEGECGACTVYLDGMAVMACLVPAA
                     RAHGAQIVTIEGLAGGDTLHPIQQAFIRTGAVQCGYCIPGFLMSGAKLLEEQTAPDQI
                     HIEQAFAGNLCRCTGYYKIIEAVHVAAETSQGNHQ"
     gene            1455..>3670
                     /locus_tag="PLC06_14290"
     CDS             1455..>3670
                     /locus_tag="PLC06_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRO91664.1"
                     /translation="MTDQILRARVTERVDAHDKVTGDARYSGDLTPPDLLFGRVLFSG
                     QPHARMLSMDTRAAEALPGVVAILTAKDVPVNEYGLGIYDQPVFVGLGSHKPHSDVSL
                     WEGDHVALVIAESEAIAARACGLIQIEWEQRPLLTDVREAMLDERVLHPWHGSNILKH
                     YKIRKGDMAAGWAAAEVIVEGEYHVPHQEHAYLQPEAGVGYIDKEGRITVEIAGQWAH
                     KDQEQVAHALGLPVDRVRIIYPAIGGAFGGREDMSLQIVLGLAAMRLNERGIRRPVRV
                     IWSRAESIIGHHKRHEGWVKTKWGATLDGKLTAVEAELILDSGSYAYTSPKVLGNAHL
                     MVTGPYEIPHAHVDSYCVTTNNIPGGAFRGFGGPQGAFAAECQMNKLAEALGIDPIDL
                     RLRNVLREGSLLTTQTPIPPGVSMAEVIEAAAEAADWDTGKITNDELRITNEERVLNP
                     SLVTSSDGRSRHSSPFQSLPPDTAHIRRGRGFACAFKNVGFSFGAPEKCEATIELHGG
                     KTIERVVLRHAGAEVGQGSHTAFKQMAAAAVGVPVELVELDLSDTATSGDSGSVSASR
                     MTFMAGNSIRQAAEEALAKWTDEDRPSIAHVTYRPPPTQPYDPDTGYTMPNFSYGYVA
                     EVVELAVDIETGLIHVERVICVDDVGKVINRTNVEGQIEGAVVQAFGYALMENLVLRD
                     ARVVNPYFSQYLIPGIRDIPERVDSVLVEIPDPIGPWGARGMAEMPFMPLAPAVVAA"
BASE COUNT          705 a         1466 c          992 g          507 t
ORIGIN      
        1 agtatgtttt catcacaagt cccccacgag atcgaacgtt acgaaatccc cgccgacctg
       61 cacaccgcgc tggccctgct ggcccaacac ggcaaggccg cccgcatcat cgccggcggc
      121 agcgacctgc tgctggagct ggaacgcggc caacgaaagg gcgtcaccac cctcatcgac
      181 atcacccgcg tccccggcct cagcgcgatc agccgcgacg aagaaggcct catccacctc
      241 ggcgcactgg tgactcacaa ccaggtcatc cactcccgcc acatcatcga ccacgccttc
      301 cccctggccc aggcctgcat cgaagtcgcc tcgccccagc tgcgcaaccg cggcaccgtc
      361 gccggcaacc tcgtcaccgc cagcccggcc aacgacacta tctccgccct gcgcgccctc
      421 aacgcccagg tcatcctcgc ctcaacccac ggctggcgca tggtcgacct gcgcgagttc
      481 tacaccggcg tccgcaaaac ggtgatgacc cccgacgaaa tggtcgtcga catcgtcatc
      541 ccccccatgc cccccaccgc ccgcggcatc ttcgtcaagc tggggctgcg ccgcgcccag
      601 gccatctccg tcgcccacct gacggccgtg ctcgacttcg ccgacgacgg ccgcgtcaat
      661 cgcgcccgca tcgctctcgg cagcgtcgcc cccaccatca tcaacgccga agccgccgaa
      721 tcatatctgc tcgacaagga gatgaccgac gacgaactga ccgtcgccgc cgcgctggtc
      781 gcctcggccg cccaccccat cgacgacgtg cgcggccccg catcctaccg cgccgaaatg
      841 atcgaagtca tggcccgccg cgccctggta gccctgcgcg acgaccgcca gcgcgaaacc
      901 tggcccgccg acccggtcat gctctggggc gccaccgatg gcatcttccc caccggcgaa
      961 cgctacgccg cgagcttcgg ccccgacacc cccatcgaaa gcaccgtcaa cggccgcgcc
     1021 gtcagcgccc ccggcatgga gaaaaccctc ttgcgctggc tgcgtgaaga agggctgctg
     1081 accggcacca aagaaggctg cgccgaaggc gaatgcggcg cctgcaccgt ctacctcgac
     1141 ggcatggccg tgatggcctg cctcgtcccc gccgcccgcg cccatggcgc acagatcgtc
     1201 accatcgaag ggctggccgg cggcgacaca ctgcacccca tccagcaagc cttcatccgg
     1261 accggcgccg tccagtgcgg ctactgcatc cccggcttcc tcatgtccgg ggccaaactg
     1321 ctggaagaac aaaccgcccc cgatcagatc cacatcgagc aagccttcgc cggcaacctc
     1381 tgccgctgca cggggtatta caagatcatc gaagccgtcc atgtggcggc cgaaacaagc
     1441 caggggaacc accaatgacc gaccagatcc tgcgcgcccg cgtcaccgag cgggtcgacg
     1501 cccatgacaa agtaaccggc gatgcccgct actccggcga cctgaccccg cccgacctgc
     1561 tcttcggccg cgtcctcttc agcggccaac cccacgcccg catgctgtcc atggacaccc
     1621 gcgccgccga ggccctgccc ggcgtcgtcg ctatcctcac cgccaaagac gtgcccgtca
     1681 acgaatacgg cctcggcatc tatgaccagc ccgtattcgt cggactgggc agccacaaac
     1741 cccactccga cgtcagcctc tgggaaggcg accacgtcgc cttggtcatc gccgaaagcg
     1801 aagccatcgc cgcccgcgcc tgtggcctga tccaaatcga atgggagcaa cgcccgctcc
     1861 tcaccgacgt ccgcgaagcc atgctcgacg aacgcgtcct ccacccctgg cacggcagca
     1921 acatcctgaa gcactacaag atccgcaagg gcgacatggc cgccggctgg gccgccgccg
     1981 aagtgatcgt cgaaggcgaa taccacgtcc cccaccagga acacgcctac ctccagcccg
     2041 aagccggcgt cggctacatc gacaaggaag gccgcatcac cgtcgagatc gccggccaat
     2101 gggcccacaa agaccaggag caggtcgccc acgccctcgg cctgcccgtc gaccgggtgc
     2161 gcatcatcta cccggccatc ggcggcgcgt tcggcggccg cgaagatatg tcactgcaga
     2221 tcgtcctcgg cctggccgcc atgcgcctca acgagcgcgg catccgccgc cccgtccgcg
     2281 tcatctggtc gcgcgccgaa agcatcatcg gccaccacaa acgccacgaa ggctgggtga
     2341 aaaccaaatg gggcgccacc ctcgacggca aactgaccgc cgtcgaagcc gaactcatcc
     2401 tcgacagcgg ctcctacgcc tacaccagcc ccaaagtgct gggcaatgcc cacctcatgg
     2461 tcaccggccc ctatgaaatc ccccacgccc acgtcgacag ctactgcgtg accaccaaca
     2521 acatccccgg cggcgccttc cgcggtttcg gcggcccaca gggcgccttc gccgccgaat
     2581 gccagatgaa caaactggcc gaagcgctcg gcatcgaccc catcgatttg cgcctcagga
     2641 acgtcctgcg cgaaggcagc ctgctgacca cccaaacccc catccccccc ggcgtcagca
     2701 tggccgaagt gatcgaggcg gcagcggagg cggcggactg ggacacaggg aaaattacga
     2761 atgacgaatt acgaattacg aatgaggagc gcgttctgaa tccgtcactc gtcacctcgt
     2821 cagacggcag gagtcgtcac tcgtcgcctt tccagagctt gccccccgac accgcccaca
     2881 tccgccgcgg ccggggcttc gcctgtgcct tcaagaacgt cggcttctcc ttcggcgcgc
     2941 cggagaaatg cgaagccacc atcgaactcc acggcggcaa gacgatcgaa cgcgtcgtcc
     3001 tgcgccatgc cggcgccgaa gtcggccagg gatcacacac cgccttcaag caaatggccg
     3061 ccgccgccgt cggcgtgccc gtcgaattgg tagaactcga cctctccgac accgccacca
     3121 gcggcgattc cggctccgtc tccgcctcgc gcatgacctt catggccggc aactctatcc
     3181 gtcaggcggc cgaagaagcc ctggccaaat ggaccgacga agaccgcccg tccatcgccc
     3241 acgtcaccta tcggccgccg ccgacccagc cctacgaccc cgacaccggc tacaccatgc
     3301 ccaacttcag ctacggctac gtggccgaag tggtcgagct ggcggtcgac atcgagaccg
     3361 gcctcatcca cgtcgagcgc gtcatctgcg tcgacgacgt cggcaaggtc atcaaccgca
     3421 ccaacgtcga gggccaaatc gaaggcgcgg tcgtccaggc tttcggctat gcgctcatgg
     3481 aaaacctcgt cctgcgcgac gcccgcgtcg tcaaccccta cttcagccaa tacctcatcc
     3541 ccggcatccg cgacatcccc gagcgcgtcg actcagtgct ggtggagata cccgacccca
     3601 tcggcccctg gggcgcgcgc ggcatggccg aaatgccctt catgcccctc gccccggccg
     3661 tcgtcgccgc
//
LOCUS       DAOMQG010000347         3669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10387_length_3669_cov_6.570282, whole genome shotgun sequence.
ACCESSION   DAOMQG010000347 DAOMQG010000000
VERSION     DAOMQG010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10387_length_3669_cov_6.570282"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..796)
                     /locus_tag="PLC06_14295"
     CDS             complement(119..796)
                     /locus_tag="PLC06_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91665.1"
                     /translation="MELRQLWKIIRRRWVLALIPAVVVLALGVATWRPAPPAYNAGVR
                     FVVGQPPGAAAAEFDEQRYYNWLTSEYIVNGLTDWVRGGKFAEMVSKRLATQGIDVPA
                     GAIQGGLAVDNARSMLTISLTAGDATQLEQMLGGVIAVLTEDNAGALPQLGGENALLT
                     QLDEPVVNPISAGIRSQLDLPLRVALALAAGLGLALLAHYLDPTVRDREEVTRAGLSI
                     LAEIPKE"
     gene            complement(769..1887)
                     /locus_tag="PLC06_14300"
     CDS             complement(769..1887)
                     /locus_tag="PLC06_14300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRO91666.1"
                     /translation="MIALIHDWLNQYGGAEDVLESLVSLYPDAPLYTALYDRQRMPAH
                     WRDWDIRPGFIDRLPLARRKPQLYFPLYPFAFEQFDLRGYDVVLSNKSGFCHGVITGP
                     ETMHVCYCLTPTRYVWRYHQYAERENLGRATRLSLAPFLTFLRQWDRLAADRVDHFIA
                     ISDEVRRRIAKVYRRDATIIYPPVDVGRFEPSSRVDDYYLFVGRLVPYRRLDVLIEAF
                     NKMGRPLVIAGSGRDRERLEALAGPTITFLGFVPDADLPDLLARCRAFVWPGDEDFGI
                     SPLQATAAGRPVIAYAAGGALETVITGPPDVATGALFAEQSVAAIIETVESFDPSSVD
                     PAVARRHAEQYDIAVFKRKIDDFVTQKYEEWNSVNSGK"
     gene            2090..>3669
                     /locus_tag="PLC06_14305"
     CDS             2090..>3669
                     /locus_tag="PLC06_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91667.1"
                     /translation="MTANPSSPLYSLTPERAARFDELQLKLAPLWRLIGRTNPGGEVQ
                     DENTLVVLPSLTMEMEIPGSHHKVYEERFLFMTFLLRQPHLRMIYLTSQPVAPLTIDY
                     YLHNLPDVTVSNARKRLHLLSPQDGSARSLAEKVLERPWFVRQIREHIPDLERAHLTP
                     FMTTDLERELALQLDIPMYAADPRHFALGGKSGARRVFAEEELQHPLGVENIRSEAGF
                     VAAIHAMRREKPAIGRVITKLNQGVSGMGNAVVDLRDLPESGAADEAEAIAARLRAMS
                     FEVPELTYADYLTGVTRQGAIVEEMISGEEMRSPSVQMRISPLGKVEILSTHDQMLGG
                     PSGQSYLGAIFPADPAYSRLITREAARIGERFVREGIIGRFALDFLTVKNEAGEWEPY
                     AIEVNLRKGGTTHPFLTLQYLTDGSYDAGTGEFTTELGERRYYVASDHVESAAYRIFT
                     PEDLLDLISVHRLTFDQTCQTGVVLHMFSGVGELGRLGVTCIHGTAARARALYDDFIA
                     LLDREAAGRSGSVVNGPS"
BASE COUNT          693 a         1215 c         1163 g          598 t
ORIGIN      
        1 actgaatact gatctactga atactgatct actgaatact gatctactga ataccgatct
       61 actgaatact gatctactga atactgatct actgaatact gatctactga atactgatct
      121 actccttcgg tatctcagcc aaaatcgata aaccggcccg cgtcacttcc tcgcggtcgc
      181 gcacggtcgg gtcgagatag tgggccagca gcgccagccc cagcccggcc gccagcgcca
      241 gcgccacccg cagcggcaaa tcaagctggc tgcggatgcc ggcgctgatg gggttgacca
      301 ccggctcatc gagctgcgtc agcagggcgt tctcgccgcc cagttgcggc agcgctccgg
      361 cgttgtcctc ggtcagcacg gcgatcacgc cgccgagcat ctgctccagt tgcgtcgcgt
      421 cgcccgccgt cagcgagata gtcagcatgc tgcgggcgtt atcgaccgcc agcccgccct
      481 gaatcgcccc cgcgggcacg tcgatcccct gcgtggccag ccgcttgctc accatttcgg
      541 cgaacttgcc gccgcgcacc cagtcggtca ggccgttgac aatatactcc gacgtcagcc
      601 agttgtagta gcgctgctcg tcgaattcgg ccgcggccgc gccgggcggc tggccgacga
      661 cgaagcgcac cccggcatta taggccggcg gcgcgggccg ccacgtcgcc acgcccaggg
      721 caaggacgac gacggccgga atgagcgcca acacccagcg acggcggatt attttccaga
      781 gttgacggag ttccattctt cgtacttctg cgtcacaaaa tcgtcgatct tccgcttgaa
      841 gacggcgatg tcatactgtt cagcgtggcg acgagccacg gccgggtcaa ccgacgacgg
      901 atcgaacgac tcgaccgtct cgatgatagc agcaaccgac tgctcggcaa acagcgcccc
      961 ggtggccacg tccggcgggc cggtaatgac cgtctccagc gcgccgccgg cggcataggc
     1021 aatgaccggc cgcccggcgg cagtggcctg caagggcgag atgccaaaat cctcatcacc
     1081 cggccagacg aaggcgcggc agcgggccag caggtcgggc aggtcggcgt cgggcacgaa
     1141 gccgaggaag gtgatcgtcg gcccggccag cgcttccagc cgctcgcggt cgcggccgct
     1201 tccggcgatg accaacggcc gccccatctt gttaaacgct tcgatcagca catccagccg
     1261 ccggtagggc acgagacggc cgacgaacag atagtaatcg tccacgcggc tcgacggctc
     1321 gaagcggccg acgtccaccg gtggataaat gatagtcgca tcgcgccggt agaccttggc
     1381 gattcgccga cgcacttcgt ccgatatggc gatgaaatgg tccacccggt cggccgccag
     1441 ccggtcccat tggcgcagga aggtgaggaa gggggccagc gacaggcgcg tggcgcggcc
     1501 gaggttctcg cgctcggcgt actggtggta gcgccagacg tagcgcgtcg gcgtcaggca
     1561 gtagcagacg tgcatcgtct ccggcccggt gatgaccccg tggcaaaagc cgctcttgtt
     1621 gctcagcacc acgtcgtagc cgcgcaggtc gaactgctcg aaggcgaacg gatagagcgg
     1681 aaagtagagc tgcggcttgc ggcgggccaa gggcagccgg tcgatgaaac cggggcggat
     1741 gtcccagtcg cgccagtggg cgggcatgcg ttggcggtca tagagggccg tatagagggg
     1801 ggcgtcggga tagagcgaca ccagcgattc cagcacgtct tccgcgccgc catactggtt
     1861 cagccagtcg tggatgaggg caatcacagg ggggattata cggtggagcg gcggaatcgt
     1921 cacaccggaa agggcgagac aaccggcaag cgacgtatta gtgactgggc gaggccgtgc
     1981 gccggttgtc ccgcccgtac atgctcccat taagcagtta gatgatctct cgtcgcgaat
     2041 cggttattct atgggggcgc agcaatccca ggcaaacaag gaacccaaca tgaccgccaa
     2101 cccgtcgtcg ccgctgtatt cattgacccc cgaacgcgcc gcccgattcg acgaactgca
     2161 actcaagctc gccccgctgt ggcggctcat cggccgcacc aaccccggcg gcgaggtgca
     2221 ggacgagaac accctcgttg tattgccctc attaaccatg gagatggaga ttcccggctc
     2281 gcatcacaag gtctatgaag agcgattcct ttttatgacc tttctcttgc ggcagccgca
     2341 cttgcggatg atctatctca cctcgcagcc ggtcgcgccg ctgaccatcg actattatct
     2401 ccacaacctg cccgacgtga ccgtgagcaa cgcccgcaag cggcttcacc tgctgtcgcc
     2461 gcaggacggc tccgcccgct cgctggccga gaaagtgctg gagcggccgt ggttcgtgcg
     2521 ccaaatccgc gagcatatcc ccgacctgga gcgcgcccat ctgaccccgt tcatgaccac
     2581 cgacctggag cgggagctgg ccctgcaact cgatatcccc atgtatgcgg ccgacccgcg
     2641 ccatttcgcc ctgggcggca aaagcggcgc gcggcgagtg ttcgccgagg aagaactgca
     2701 acacccgctg ggcgtggaga acatccgcag cgaggccggg ttcgtggccg cgatccacgc
     2761 catgcgccgc gagaagccgg ccatcggccg cgtcatcacc aagctgaatc agggcgtgtc
     2821 gggcatgggc aacgccgtag tcgacttgcg cgacctgccg gaatcgggcg cggccgacga
     2881 ggccgaggcc atcgccgcgc ggctgcgggc catgtcgttc gaagttcctg agctgaccta
     2941 cgccgactac ctgacgggcg tgacccgcca gggggccatc gtcgaggaga tgatcagcgg
     3001 cgaggagatg cgcagcccca gcgtgcagat gcgtatctcg ccgctgggca aggtcgaaat
     3061 cctgtccacc cacgaccaga tgctcggcgg ccccagcggc cagagctatc tgggagccat
     3121 cttcccggcc gacccggcct acagccgcct catcacccgc gaggcggcca ggatcggcga
     3181 gcggttcgtg cgcgagggga ttatcggccg atttgcgctc gatttcctga ccgtgaagaa
     3241 cgaggccggt gaatgggaac cctacgccat cgaggtgaac ctgcgcaagg ggggcacaac
     3301 ccacccgttt ctgacgctgc aatatctgac cgatggaagc tacgacgccg ggacgggcga
     3361 attcaccacc gaactgggcg agcggcgcta ttacgtggcg agtgaccatg tggaatcggc
     3421 cgcctatcgc atcttcaccc ccgaagacct gctcgacctg atcagcgtcc atcgcctgac
     3481 gttcgatcag acctgccaga cgggcgtcgt gctgcacatg ttcagcggcg tcggcgaact
     3541 cggccgcctg ggggtcacct gcatccacgg cacggccgcc cgcgcccgcg ccctctacga
     3601 cgacttcatc gccctgctcg accgcgaggc ggcggggaga agtggatcgg tggtcaatgg
     3661 tccgtccca
//
LOCUS       DAOMQG010000348         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10489_length_3636_cov_10.734152, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000348 DAOMQG010000000
VERSION     DAOMQG010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10489_length_3636_cov_10.734152"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..574)
                     /locus_tag="PLC06_14310"
     CDS             complement(2..574)
                     /locus_tag="PLC06_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91668.1"
                     /translation="MIENEAAAEAIHEILSELEVPYAIIGGMAVQHWGEARYTGDLDL
                     TVFVPIEDQEAFLVTISNRLVPRREGALEFAQKHRIFLAQTGSGFPVDISLGLPGYEE
                     GMVSNAVDYKTGSGAIVRLCSAEDLIIHKLIAGRPQDIRDIQGIIIRRASSLDLPYIR
                     QWLKIFSEWLESDDVIDRFEQAWLKYGPAS"
     gene            complement(571..861)
                     /locus_tag="PLC06_14315"
     CDS             complement(571..861)
                     /locus_tag="PLC06_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91669.1"
                     /translation="MTDPATIAARNRQLIRETIAGYAAAAEVIEQERIERLRQITDEE
                     SRADYAMLLSFHRLFKNESDKEGLERLEQWRLQEKIAVRQVFEKAARAGGWI"
     gene            1040..1762
                     /locus_tag="PLC06_14320"
     CDS             1040..1762
                     /locus_tag="PLC06_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91670.1"
                     /translation="MAKQVLVVDDEKAITSALLVLLNGAGYEVVIAHSGEEAIRQLSA
                     GKPDLIILDVMMPGLDGYQVSRQVRQRDEYIPILMLTAKDESWEKVTGLELGADAYMT
                     KPFDSGELLAQVKALLRLAGQASSAIEKGEDSGAGPLICGPLTLYPHQHRTLLNEREI
                     QLSPREFDLLHFFMRHPGQVFGRQTLLRQVWAYDYPDDSRTIDTHIGRLRAKIETDPA
                     QPQLLQTVRGFGYRLAARSDER"
     gene            complement(1820..2254)
                     /locus_tag="PLC06_14325"
     CDS             complement(1820..2254)
                     /locus_tag="PLC06_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91671.1"
                     /translation="MKVTFFILVLLGLTACGTKPHEPQVPTSVATPPIISARLLAEMV
                     GELTIENECLRINDYLLVWPPDFTVDIKEDMVEIRDEFTGEKIVWRSGETVQVGGGEV
                     SYPSLDEQVRQRTPAHCSKGGAGAFWLVGDIVIPATVTPAAK"
     gene            complement(2351..2671)
                     /locus_tag="PLC06_14330"
     CDS             complement(2351..2671)
                     /locus_tag="PLC06_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRO91672.1"
                     /translation="MAKVTAGRDALGDFAPKFAELNDDVLFGQVWLREEQLSARDRSM
                     ITVTVLMASGILDNSLQYHLQTARANGITKEEIAEILTHAAFYAGWPKAWAAFRMAKE
                     IWTD"
     gene            complement(2689..3090)
                     /locus_tag="PLC06_14335"
     CDS             complement(2689..3090)
                     /locus_tag="PLC06_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRO91673.1"
                     /translation="MSDEFDTIFPMGGKNEAYAQYFVGQSYLNMLSTQGVAIGNVTFE
                     PGCRNNWHIHHKGGQILLCTAGRGYFQEWEKPAQELHPGDVVHISPGVKHWHGAAPDS
                     WFAHLAVEIPAQGASNEWLEPVSEDDYGKLR"
     gene            complement(3090..3362)
                     /locus_tag="PLC06_14340"
                     /pseudo
     CDS             complement(3090..3362)
                     /locus_tag="PLC06_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT          833 a         1017 c          930 g          856 t
ORIGIN      
        1 ttcatgatgc cggcccgtac ttgagccagg cttgctcgaa gcggtcgatg acgtcgtcgc
       61 tttcgagcca ctcggaaaat attttcagcc actgccgaat atatggcagg tcgagtgacg
      121 aagctcgccg aatgataatg ccttgaatat cgcgaatgtc ctgcggtcgt ccggcaatta
      181 acttgtggat gatcaaatct tcggccgagc ataagcgtac aatcgcgccg ctccccgtct
      241 tatagtcaac cgcattggag accatcccct cttcatatcc cggcaatccc aatgaaatat
      301 ccaccgggaa cccgctgccc gtttgcgcta aaaagatacg atgcttttgg gcaaactcaa
      361 gggcaccctc gcgacgggga accaatcgat ttgatatcgt taccagaaag gcttcttgat
      421 cttcaatcgg gacgaagacc gtgagatcga ggtctccggt ataacgggcc tcgccccaat
      481 gttggacggc cataccacca atgatggcat atggaacctc aagttcagac aaaatctcgt
      541 gaatggcttc ggcggccgcc tcgttctcaa tcaaatccag cccccggcgc gagccgcttt
      601 ttcaaagacc tgacgcacgg cgatcttctc ttgaaggcgc cattgttcaa ggcgttcaag
      661 tccttccttg tcggattcat ttttaaacag ccggtggaag ctcaacagca tcgcgtaatc
      721 cgcccttgat tcttcgtccg taatttggcg caatcgttca atccgctcct gctcaatcac
      781 ttcggccgcg gcggcgtagc ccgcaatcgt ctcgcggatg agctgcctgt ttcgggcggc
      841 aatcgtggcg gggtcggtca tggtgactct gtcggatact tcccggtggc ggcaaaaggt
      901 gcggccggtg ggcaagacaa gataaatcgc ctcaatctga tatgatagga tgagacagga
      961 atctcaaacc acggatggga actctcagcc tatatcatag tcccgatcgc gatatttcca
     1021 aaattcgggg aagagcacaa tggccaagca agtattagtt gttgatgacg aaaaggcgat
     1081 aaccagcgcg ctgctggtat tgctcaacgg cgccggctac gaagttgtca ttgcccacag
     1141 cggggaagag gcgatccggc agctatccgc cggcaagcct gatctgatca ttctggatgt
     1201 catgatgccc ggcctcgacg gctatcaggt cagccggcag gttcggcaac gggatgaata
     1261 catacccatt ctcatgttga cggccaaaga tgaatcgtgg gaaaaagtga ccggccttga
     1321 actgggagcc gacgcttaca tgaccaaacc gtttgattcc ggtgaattgc tggcccaggt
     1381 gaaggcgctc ttgcgattgg ccggacaagc aagctccgcc attgagaagg gagaggacag
     1441 cggcgccggg cctttgattt gcgggccact cacgctctac ccccaccagc atcgaacatt
     1501 gttgaacgag agggaaatcc agttatcacc aagggagttc gatctgctcc atttctttat
     1561 gcggcatccc ggtcaggtat tcggccggca aaccttattg cgtcaggtgt gggcctatga
     1621 ctatccggat gactctcgca caatcgatac gcacatcggg cgcctgcggg ccaaaattga
     1681 aaccgatccc gcgcagcccc agctactgca aacagtgcgc ggttttggct atcggctggc
     1741 ggcgcgttcg gatgagcggt gatcgaactg tatagcaggg gaaccatatc atctcctgtt
     1801 gcaaatagaa tatggcttgc tatttagccg ctggtgtaac tgttgccggg attactatgt
     1861 ctcccacaag ccaaaaagcg cccgccccgc ccttcgagca atgtgcgggc gtgcgttggc
     1921 gtacttgctc atccagacta ggatacgata cttcgccgcc tccaacttgc actgtctctc
     1981 cgctgcgcca tacgatcttc tcgcctgtga actcgtctct gatctcaacc atgtcttctt
     2041 tgatgtcgac tgtaaaatcg ggcggccata caagtaagta gtcattaatt cgcagacatt
     2101 cattttctat cgttagctcg ccaaccatct cagccaataa acgcgctgaa ataatcggcg
     2161 gtgttgcaac agaagtggga acttggggtt catggggttt tgtaccacag gctgttaaac
     2221 caagtaatac caaaataaaa aaggtaactt tcatagtctc tcgcagagca tggtgacctg
     2281 tcggacctta ttccgcgccg attgtccgcg cctcggggat cgttttcacc ggcttgccca
     2341 cacacttccg ttaatctgtc cagatctctt ttgccatgcg gaacgccgcc catgccttgg
     2401 gccatccggc ataaaaagcc gcatgggtca gtatttccgc aatttcctct ttagtgatgc
     2461 cattggctct ggcggtctgc aaatgatatt gcaaggagtt gtccagaata ccactcgcca
     2521 tcagaaccgt cacggtaatc atgctgcggt ctctggcaga tagctgttcc tcccgtaacc
     2581 atacttgccc gaacagtaca tcgtcgttca gctccgcaaa cttgggggca aagtctccca
     2641 aagcatctcg gcctgctgta acctttgcca taactttctt tcctttctct atctcagctt
     2701 tccgtagtcg tcctcactga ccggctccag ccactcattg gaagcgccct gtgcaggaat
     2761 ctccacggca agatgggcga accaactgtc cggcgcggct ccgtgccagt gcttcacccc
     2821 cggcgagata tggacgacat cgcccgggtg cagctcctgc gcgggcttct cccattcctg
     2881 aaagtagccg cgcccggccg tgcagagcaa aatctgcccg cccttgtggt ggatatgcca
     2941 gttgttgcgg caccccggct caaaggtcac gttgccgatg gcgacgcctt gcgtggatag
     3001 catattcaga taactctgac cgacaaaata ctgtgcatag gcttcgtttt tcccacccat
     3061 ggggaagatg gtatcgaatt catcgctcat tacagttttc ctccaaaaac cggcatggca
     3121 ttggcctcgc cgtaatccgt taactgctcc gcgtttttca gcagttccat atcctcatca
     3181 gaaatgacaa aatcgacagc ggcgtttgtt ttcatatgtg ccgggttcgc cgttttgggc
     3241 agcggcaata acccaagctg caaacaataa cggatactca actgcgggat ggaaacggca
     3301 tatttttcag ccattatttt catttgccga tgagtcaggg cagcgccgtg ggcgatgggt
     3361 gagacaagat aaatcgcctc aatctgatat gataggatga gacaggaatc tcaaaccacg
     3421 gatgggaact ctcagcctat atcatagtcc cgatcgggat atttccaaaa ttcagggaag
     3481 agcacaatgg ccaaacaatt actggttgtt gacgacgcca ggcgtagtag ccggcatgcg
     3541 atacgcccaa tgtcgcgcat atcagcccca ccgggtagcg tccgcgctgc gtatcgatga
     3601 atcggtacct catgggtttc cctggcgaag aacgtg
//
LOCUS       DAOMQG010000349         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10532_length_3621_cov_11.025519, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000349 DAOMQG010000000
VERSION     DAOMQG010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_3621_cov_11.025519"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLC06_14345"
     CDS             <1..306
                     /locus_tag="PLC06_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRO91674.1"
                     /translation="HGDLEPGNLLLRDGRLSAVIDFCCLCLGDPAVDLIPAWSIFTGE
                     ARAAFRSGLDVDDAAWARGKGWALSTAIIALPYYVNTNPFMAANARRKLAAVLAEQR"
     gene            437..1246
                     /locus_tag="PLC06_14350"
     CDS             437..1246
                     /locus_tag="PLC06_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO91675.1"
                     /translation="MKAANKVIVVTGAGSGMGRELVFALVKKGAHVAAVDLNEVTLKE
                     TIALVGQHGSKVSSHVVNVSDRLAVEALPDEVIAAHGAVDGVINNAGIIQPFVRLNDL
                     QYDAIERVLNVNLYGVIYMTKAFLPHLLTRPTAHIVNVSSMGGFFPVPGQTVYGASKA
                     AVKLMTEGLYAELLETNVRVTAVFPGAIATNITQNSGVAIDRPTTDNAEQSFPMTSAE
                     DAAAIIIDGMERDQFQVYVGRDAKMMNLFYRLSPRRATQFMYSRMKELLGD"
     assembly_gap    1351..1450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1612..2646)
                     /locus_tag="PLC06_14355"
     CDS             complement(1612..2646)
                     /locus_tag="PLC06_14355"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HRO91676.1"
                     /translation="MQYEITQPGPLLDENGRLAHVGWSRQPLLDCNLEQARFYPLGLF
                     QRFRKKRWDYYAVFTPRRFFSATIADLGYAANIFVYTLDFATGDLHEEGLVVPFGRNV
                     TLERNSLEGDATYTSGKASLRFVARPKERYISVDWPGFHDGRGIHADISLAVPPGHES
                     MTIVIPIGARRFYYNTKINCLPATGQIRYGDDLEELRPDSSLGQLDWGRGVWEYSSFW
                     NWASASGFLPDGRTIGLNLGRGFGDLSRATENAVILDGRVHKLGDVTFGYTSDDFMRP
                     WRFTDEEQRLDLTFTPFKDRTARTNLGVIFSEVHQMFGRYNGRVVLDDGTTLEIRDLI
                     GFAEEHRARW"
     gene            complement(2659..>3621)
                     /locus_tag="PLC06_14360"
     CDS             complement(2659..>3621)
                     /locus_tag="PLC06_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO91677.1"
                     /translation="PASEHVERVIIPQRRLWTHLRLGRELRRRPPDVFFTPAHVIPIG
                     YHGAAVATVHDLGYEQFPEAHPWRQLAYLRWSTRHNARVARRVIADSIATRDDLMRFY
                     ATDAAKIDVVYPGADPDLRRVDDKVKIADALTRHGIRPPYLLYLGTLQPRKNLIRLVE
                     AFAASELRDQGYSLVLIGKQGWLAAPLLDAIGSLPSAVRDRVLLPGYIDEIDKSPLIS
                     GATALVFPSLYEGFGFPVLEAQACGAPVICANTSSLPEVAGQGAMLVDPLDTTALAEA
                     MRRVAGDSDLQHKLITAGYANLARFSWPDAATRVLDVLEAIVVS"
BASE COUNT          746 a         1097 c         1054 g          624 t
ORIGIN      
        1 cacggcgatc tggaaccggg caacctgctg ctgcgcgacg gccgcctgag cgccgtcatc
       61 gacttctgct gcctgtgcct gggtgatccg gccgtcgacc tcatccccgc ctggagcatc
      121 ttcaccggcg aagcccgcgc cgcctttcgc tccgggttgg acgttgatga cgccgcctgg
      181 gcacgcggca aaggctgggc gctttccaca gccatcatcg ccctgcccta ctacgtgaac
      241 accaacccct tcatggccgc caacgcgcga cgcaagctcg cggcggtgct ggctgagcaa
      301 aggtaaatag cccgtggatc aatcctcgtc ccactctgcc ccctgcccgc taccgccatc
      361 ttcttagcct ggctccccat cctgcgataa gatatcaacc cgaatcatca agataattac
      421 acaaggggac aaaccaatga aagcagccaa caaagtcatc gtcgtgacgg gagcgggaag
      481 cggcatgggg cgcgagttag tgttcgcact cgtgaaaaaa ggcgctcatg tggccgccgt
      541 ggatctcaac gaggtaactc tcaaagaaac tatcgcgctg gtcgggcagc acggcagcaa
      601 ggtcagttcc cacgtggtga acgtgagcga tcgcctagcc gtggaagcac tgccggacga
      661 ggtcatcgcc gcccatggcg cggtagacgg cgtcatcaac aatgccggga tcatccagcc
      721 ctttgtgcgc ctcaacgacc tgcagtacga cgccatcgag cgtgtgctca acgtcaacct
      781 ctatggcgtc atctacatga ccaaggcctt tctgccccac ctgctgacgc ggcccaccgc
      841 tcacatcgtc aacgtctcca gcatgggcgg cttcttcccc gtgccggggc agacggtgta
      901 tggcgcgtcg aaggcggccg tcaagctgat gaccgagggg ttatatgccg aactgcttga
      961 aaccaacgtc cgggtgaccg cggttttccc cggagccatc gcgacgaata tcacccagaa
     1021 ttccggcgta gccatcgacc ggccaacaac cgataatgcg gagcagagct ttcccatgac
     1081 ctcggccgaa gacgcggcgg caatcatcat cgacgggatg gagcgcgacc aattccaggt
     1141 ctacgtcggc cgcgacgcga agatgatgaa cctgttttat cgcctcagcc ctcgacgggc
     1201 cacacaattc atgtatagcc ggatgaagga gctattagga gattagtcca tgacgttcag
     1261 caggtggcat caatggatgt atcgtggcgg ccgcccgaac atcatggcga gggttatcaa
     1321 tagcctgacg gcgcgaatcc attcgacagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn ttccacatcg tcccgcgcat ggccgaccag ccggatgacc ggcgcagcac
     1501 gaagatattc gattatttag gggtgccgga ggaagagcgg gtgactgaag agcggatgaa
     1561 cgagatcgct tggcgaactc gagcaattct acaggcataa cccaaaagtg tttaccaccg
     1621 cgcccggtgc tcctcggcaa aaccgatgag gtcgcgtatt tccagcgtcg tgccgtcatc
     1681 cagcaccacc cggccattgt agcggccaaa catctggtgc acctcgctga agatgacgcc
     1741 cagattcgtc cgcgccgtgc gatccttgaa cggcgtgaag gtcagatcga gccgttgctc
     1801 ctcatcagta aagcgccacg gccgcatgaa gtcgtcgcta gtgtagccaa aagtcacatc
     1861 accgagcttg tgaacgcggc cgtcgaggat gacggcgttc tccgtcgccc ggctcaggtc
     1921 gccgaagccg cggccgaggt tcagtccaat cgtccggccg tccggcagga agcctgaggc
     1981 gctggcccag ttccagaagc tgctgtactc ccacacgccg cgcccccaat ccagctggcc
     2041 gagactgcta tccggcctga gttcttccaa gtcatcacca taccgaattt ggcccgtcgc
     2101 cggaaggcag ttgatcttgg tgttgtagta gaagcgccgg gcgccaatgg gaatgacgat
     2161 ggtcatcgac tcgtgccccg gcgggacggc caggctgatg tcggcgtgga tgccgcggcc
     2221 gtcgtggaag ccgggccagt cgacgctgat atatcgttcc tttggcctgg ccacaaagcg
     2281 cagcgatgcc ttgccgctcg tatacgtcgc atctccttcc aggctgttgc gctccagagt
     2341 cacattgcgg ccgaacggca ccaccagccc ttcctcgtgc aggtcgccgg tagcgaagtc
     2401 aagcgtgtag acgaagatat tggccgcata acccagatcg gcgatggtgg ccgagaaaaa
     2461 gcgacgcggg gtgaagacgg cgtagtaatc ccagcgcttc ttgcgaaatc tttggaacag
     2521 gcccagcgga tagaaacgcg cctgttcgag gttgcaatcc agcaacggct ggcgcgacca
     2581 gcccacatgg gccaggcggc cattctcatc gagcaaagga ccgggttgcg taatttcata
     2641 ttgcataata aggctccctc aagatacgac gattgcttcc agaacatcaa ggacacgggt
     2701 ggccgcatcc ggccacgaga aacgggcaag attggcataa ccggccgtaa tcagtttatg
     2761 ctgaagatct gaatcgcctg ccactcgccg cattgcctcg gccagcgcgg tcgtgtccag
     2821 cggatcaacc aacatcgccc cctgccccgc cacctccggc agagaactgg tattggcgca
     2881 gataaccggc gcgccgcaag cctgcgcctc cagcaccgga aaaccaaacc cctcatagag
     2941 cgatgggaat accagcgccg tcgcgccgga gatcaatggg cttttgtcta tctcgtcgat
     3001 gtaaccgggg aggaggacgc gatcgcgaac ggccgacggc aggctaccaa tcgcatccaa
     3061 cagcggagca gccaaccacc cctgcttgcc gatcaggaca agcgagtagc cctgatcgcg
     3121 taactcgctc gcggcgaagg cctcgaccag ccggatgagg ttcttgcgcg gctgcagcgt
     3181 gcccagataa agcagataag gcggccgaat gccatgacga gtcagagcat cggcgatctt
     3241 caccttgtca tcgacacggc gcaggtcggg atcggctccg ggatatacga cgtcgatttt
     3301 ggccgcatcc gtggcgtaga accgcatcag gtcgtcgcgc gtggcgatag aatccgcgat
     3361 cacccgacga gcgacacggg cattgtggcg cgtactccag cgcaggtaag ccagttgtcg
     3421 ccacgggtgc gcttcgggga actgttcgta gcccagatcg tggacggtag ccacggccgc
     3481 accatgatag ccgatgggaa tgacgtgggc gggggtgaag aaaacgtcgg gcggccgccg
     3541 gcgcagttcg cggccgaggc gcagatgcgt ccatagtcgg cgctggggga tgattacgcg
     3601 ttcgacgtgt tccgaagccg g
//
LOCUS       DAOMQG010000350         3607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10579_length_3607_cov_10.930180, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000350 DAOMQG010000000
VERSION     DAOMQG010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10579_length_3607_cov_10.930180"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLC06_14365"
     CDS             <1..305
                     /locus_tag="PLC06_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91678.1"
                     /translation="ALLGAVYLRFHSEKRVEKSVRSGYVCRVRSTSLPLCKIRTIAVV
                     RRINKSPFAFPDRACSMTCRNPSNFSRNIVDGVLWMMALFSTKPPGRDHPGGQAAQ"
     gene            545..1456
                     /gene="gnd"
                     /locus_tag="PLC06_14370"
     CDS             545..1456
                     /gene="gnd"
                     /locus_tag="PLC06_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRO91679.1"
                     /translation="MDIAMIGLGRMGGNMASRLLRGGHRVVVYNRSSGPIHAAEAEGA
                     EGAGSIEDVVAKLAAPRAVWLMLPAGDVTEAAVTQLADLLAVGDVIIEGGNSNYKDSM
                     RRAAFLADKGIYMVDSGTSGGIWGLREGYSLMVGGRAEAVELVRPALETLAPAADRGW
                     AHVGPSGAGHFVKMIHNGIEYGMMQAYAEGFEILRAKEEFNLDLHQIAELWRYGSVVR
                     SWLLDLTAAALADDPELSDLRGWVADSGEGRWTVFEAIDQDVPAPVITLALQMRFVSR
                     QDESYAAKLLAAMRNQFGGHAVKTVGE"
     gene            complement(1546..3585)
                     /locus_tag="PLC06_14375"
     CDS             complement(1546..3585)
                     /locus_tag="PLC06_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRO91680.1"
                     /translation="MLSPFEKTLFVLLVIICLTAVANTFAMMARVINRGQRALYLDEL
                     PRRVMAGIMALITQGRIIRHRKLTSLFHYGVAFGFIFYGLVNVIDVLEGLIPGFQFFP
                     DNIIGHLFRLAADLFAAAVIIGVAYFLLRRFAAQDPALKTRDNVKLMDRVREGGIAVD
                     SLIVGLFILTHVGSRLLGASFHLAQHGPDPWQPVAGFIASTFLGGLSPEVLTFGWHLF
                     WWLAVGLILIFLPYFPYTKHAHLFMGPLNFMVSPERTYLGQMFKLDLDDETIEQFGAN
                     VLTDLPKKHILDAYACIMCNRCQEVCPAYTTGKELSPAALEINKRYYIRDNMAALASG
                     ATGGISLIDFAISESAVWACTACGHCIEVCPVGNVPMLDIMEIRRDQVMMQSEFPDQL
                     KGAFIGMERMGNPWQAQGSRMEWAAPLPFDVPTVAENPDYEYLLWIGCAQAFNPDAQK
                     TIRSLATIMHEAGVNFAVLGDDEVCNGDAARRAGNEPLFQAMAETNIETLNAAKANER
                     RIVTSCPHCFTTIGKEYGELGGHYTMFHHTQLISDLIGRGKLQFNSKIMEDVTFHDPC
                     YLGRHNGVIEEPRTTLTATGITLTEMPRHGKDSFCCGAGGAQYWKEEEHGVTGEKVNI
                     NRYREAKATGAAVLAVGCPFCHTMMSDANREEGDGMKVIDVAELVVEAMSLPVIN"
BASE COUNT          718 a          911 c         1223 g          755 t
ORIGIN      
        1 gcgccctcct gggcgcggtt tatttacggt ttcattctga aaaaagggtt gaaaaatcgg
       61 ttcggtcggg ttatgtctgt cgtgtcagga gcacctctct tccactatgc aagattcgga
      121 cgattgcagt cgtccgccgg ataaacaaat ctccttttgc ctttccagac agggcctgtt
      181 caatgacctg tcgcaatcct tcaaatttta gtagaaacat cgttgatggc gtgctttgga
      241 tgatggctct attcagcacg aaacctcccg gccgagatca tcccggcggg caggcggcgc
      301 aatgaaaata gacgcagatg tcgatgcctg gttccctgtc ccgattataa ctattcggat
      361 catcatggcg atgtgccgtt gaacgcaccc caccgagcat agtataagag attaggagga
      421 tgggatcaat tcaaaaacag gggaatacaa atttagaaga aatggcctga aatcgcccaa
      481 tattatttgt tcgtatacat tatgatgaga cgaaagttat gtgcgacaag atgaggagat
      541 tgaaatggat atcgcgatga tcggactggg caggatgggc gggaacatgg cttcgcggct
      601 gttgcgcggc gggcatcggg tcgttgtcta taaccggtcg tcagggccga ttcatgccgc
      661 cgaggctgaa ggcgcagagg gagcgggatc gattgaagac gtggttgcga agctcgcggc
      721 gccacgggcg gtgtggctga tgttgcccgc gggtgatgtc accgaagcgg ccgtaaccca
      781 actggccgat ctgttggctg taggggacgt gatcatcgag gggggcaaca gcaattataa
      841 agactccatg cgccgtgcag ccttcctggc cgataaaggt atctacatgg tcgatagcgg
      901 aacgagcggc ggcatatggg gtctgcgtga ggggtatagc ctgatggttg gcggccgagc
      961 agaagcggtg gagcttgtcc gcccggcgct ggagacgctg gccccggcgg ccgatcgggg
     1021 ttgggcgcat gtaggccctt cgggtgcggg tcatttcgtg aagatgatcc ataacggcat
     1081 cgagtacggc atgatgcagg cctacgccga ggggttcgag attctgcggg ccaaggagga
     1141 gttcaatctc gatctgcacc agatcgccga gttgtggcgt tatggcagcg tggtgcgctc
     1201 gtggctgctg gacctgacgg ccgcggcgct ggccgacgac cccgagctaa gcgatcttcg
     1261 tggctgggtg gccgatagcg gtgaagggcg ctggacggtg ttcgaagcga ttgatcagga
     1321 tgtgcccgcg ccggtgatta cgctggcgct gcagatgcgt ttcgtcagtc gccaggatga
     1381 gagttatgcg gccaagctgc tggcggcgat gcgtaaccag ttcggcggtc atgccgtgaa
     1441 gacggttggg gagtgatcga taaagacctg acaggttggc aattaagggt gcggtcggtc
     1501 ggataatgta tcgccaacct gtcaggtctg tatgcggcgg cagctttagt tgattaccgg
     1561 cagggacatg gcctcgacga cgagttcggc cacgtcgatg accttcatgc cgtcaccttc
     1621 ctcgcggttg gcgtcgctca tcatcgtgtg gcagaagggg cagccgacgg ccagcacggc
     1681 cgcgccggtg gctttggctt cgcggtagcg gttgatgttg accttttcgc ccgtgacacc
     1741 atgttcctct tccttccagt attgcgcgcc gcccgcgccg cagcagaagg agtctttgcc
     1801 gtggcggggc atttcggtga gcgtgatccc ggtggcggtc agcgtggtgc gcggttcttc
     1861 gatcacccca ttgtggcggc cgagataaca cggatcatgg aaggtcacgt cttccatgat
     1921 tttgctattg aactgtaact tgccgcggcc gatcagatcg gagatgagct gggtgtggtg
     1981 gaacatggtg taatggccgc ccagttcgcc gtactccttg ccgatagtgg tgaagcagtg
     2041 ggggcaactg gtgacgatgc ggcgctcgtt ggctttggcc gcgttgagcg tttcgatgtt
     2101 ggtttcggcc atagcctgga agaggggctc attccccgcc cgacgagcag cgtctccgtt
     2161 gcagacttca tcatcgccca ggacggcgaa gttaacgccc gcttcgtgca taatcgttgc
     2221 cagggagcga atagtctttt gtgcgtccgg gttaaatgcc tgggcgcagc cgatccacag
     2281 taaatactcg taatcggggt tctcggccac ggtgggtaca tcgaaaggca acggtgccgc
     2341 ccactccatt cgactgcctt gtgcttgcca tgggttaccc atgcgctcca tgccgataaa
     2401 agcgcccttt aactgatcgg ggaattcgct ttgcatcatc acttggtcgc ggcggatctc
     2461 catgatgtcg agcatgggga cgttaccgac ggggcagact tcgatgcagt gaccgcaggc
     2521 cgtgcaggcc caaacggcgc tctcgctgat ggcgaagtcg atgagcgaga tgccgccggt
     2581 tgcgcccgag gccagggcgg ccatattgtc gcgaatgtag tagcgtttgt tgatctccag
     2641 cgcggccggg gatagctcct tgccggtggt gtaggccggg cagacttcct ggcagcggtt
     2701 gcacatgatg caggcgtagg catcgagaat gtgttttttg ggcaggtcgg tgaggacatt
     2761 ggcgccgaac tgctcgatgg tctcgtcgtc gagatcgagc ttgaacatct gccccagata
     2821 ggtgcgctcc gggctgacca tgaagttgag cggccccatg aacaggtggg cgtgtttggt
     2881 ataggggaag tagggcagga agatcaggat gaggccgacg gcgagccacc agaagagatg
     2941 ccagccgaac gtcagcactt ccggcgagag gccgcccagg aaagtggagg cgatgaagcc
     3001 ggcgacgggt tgccacggat cggggccgtg ttgagcgagg tggaacgacg cgccgagcag
     3061 ccgcgagccg acgtgggtca ggatgaacag gccgacgatg agcgagtcga cggcgatgcc
     3121 gccctcgcga acgcgatcca tcagcttgac gttgtcgcgg gttttgaggg ccgggtcctg
     3181 agctgcgaaa cgacgcagca ggaagtaggc gacgccgatg atgaccgcgg cggcgaacag
     3241 gtcggccgcc agccggaaca ggtgaccgat gatattgtcg ggaaagaatt ggaagcccgg
     3301 tatcaaacct tcgagcacgt cgataacgtt gaccaggcca tagaagatga agccgaaggc
     3361 gacgccgtag tggaacaatg aggtcagttt gcggtggcgg atgatgcggc cctgagtgat
     3421 gagagccata atgccggcca tgacgcggcg aggcagctcg tcgaggtaga gcgcgcgttg
     3481 gccgcggttg atgacgcggg ccatcatggc gaaggtgttg gcgacggccg tgaggcagat
     3541 gatgaccaac agcacgaaca gggttttttc gaagggagac agcatgttct cttcctctcc
     3601 tttcgcc
//
LOCUS       DAOMQG010000351         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10605_length_3600_cov_6.366432, whole genome shotgun sequence.
ACCESSION   DAOMQG010000351 DAOMQG010000000
VERSION     DAOMQG010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10605_length_3600_cov_6.366432"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..2268
                     /locus_tag="PLC06_14380"
     CDS             22..2268
                     /locus_tag="PLC06_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HRO91681.1"
                     /translation="MRKVLKIFGIIIVVIVALVLLAFAVAAAVPPAVDPPVDMAHHGA
                     GSSSVEPAYTGLQREYPPSNEPADNPTTPEKAELGRLLFFDPVLSENNDIACATCHHP
                     DYGFSDGRATSMGTSGVELPRNALTLWDVAYVKSLFWDGRVDNLEDQALVPLTHADEM
                     AVSDPAAMAAELAAIPEYAALFDAAFGGGAAAVTADNTARALAAFQRTLISDDAPFDR
                     YAAGDFDALTTQQRRGLALFRSGALRCFECHGAPTFASDTFRVIGVDSDDPGRAAVVD
                     EGLFGAFKVPTLRNIALSAPYMHDGSMATLEEVVKFYEGGGGRATGFEQVDVFVNPFE
                     LSDQDRADLISFLYALTDESNKPEIPAAVPSGLAVVAPYDNPARAEVALHNTAAGGAV
                     VEDGPPQTITVQPGETIQSAVDRARPGDTVEIPYGVYHERVVIDMSDFTLVGVPNEAG
                     EFPILDGEGKLPEGVIASGNNFSVGNLHTRNYTDNGVLVEGVTGVYFHDIFAENVGTY
                     GVYPVRSTDVLIERVTVTGVDDAGIYAGQSENVTVRDSVAYGNVIGIELENTLGGELY
                     NNHLYDNSNGILLVLLPQLSSKISSGAIVRDNLVENNNHPNFAPEGGMARIVPPGTGI
                     LIIGSDNNEVFNNTITGNKTAGIAVFSLTGTGAFNENELDVGPLAENNFTHNNTFSNN
                     GFDPDPTVSELGIPAGDILWDTTGANNRFNESGVSGFPPLLPGDNWPGFVRNAYGNFW
                     RVLLKVMG"
     gene            complement(2433..3578)
                     /locus_tag="PLC06_14385"
     CDS             complement(2433..3578)
                     /locus_tag="PLC06_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major facilitator superfamily domain-containing
                     protein 6"
                     /protein_id="HRO91682.1"
                     /translation="MRPKSVYFFYYAALGCLAPFMALYYAELGMTGAQIGVLSGTIPL
                     VNLFSAPFWGGIADATRRHRAVLLTTIAGLGGSAVVLLLADTFVELLMAVIVYAFFVG
                     PIVPLVDNAVMAFLGDRKSDYGRVRLWGSVGWGTTGLIIGSLIERSGLAIGFYGFMAI
                     MAVTFLAATRLPMDIGERARHSYSAGLGVLLRNGRFLLLLLVSLVFGVTLGVMLSYQF
                     LYIAELGGSRTLMAWTMTANTLSEIPFLFISAGLLRRFGSSKMIALALAFTVVRNLAM
                     GIVTDPWLVLPISLLHGPSFAVLWAAGVADADAAAPRGLGATAQALFSSMMFGLGAAL
                     GGFLGGPAYEAIGFAALYSLLGWLALGMLIVFVAARFADRRRTAADR"
BASE COUNT          682 a         1230 c         1164 g          524 t
ORIGIN      
        1 gatcactaaa aaggaggagc aatgcgcaag gtactcaaga tattcggaat tattatcgtc
       61 gtcatcgtcg cgctggtgct gctggcgttt gccgtggccg cggccgtgcc gcccgccgtg
      121 gacccgccgg tcgatatggc ccaccacggc gcgggttcca gcagcgtgga gccggcctac
      181 accggcttgc agcgcgaata cccgcccagc aacgagccgg ccgacaaccc gaccacgccg
      241 gagaaggccg agttggggcg cctcctcttc ttcgaccccg tcctgtcgga gaacaacgac
      301 atcgcctgcg ccacctgcca ccatcctgac tatggcttca gcgacggccg cgcgacctcg
      361 atgggcacca gtggcgtcga gctgccccgc aacgccctga ccttgtggga cgtggcctat
      421 gtgaagagcc tgttctggga cggccgcgtc gataatctgg aagaccaggc ccttgtgccg
      481 ctgacccatg ccgacgagat ggccgtgagc gacccggcgg cgatggccgc ggaactggcc
      541 gccatccccg aatatgccgc cctgttcgac gcggcgtttg gcggcggcgc ggccgcggtg
      601 acggccgaca acaccgcccg cgccctggcc gccttccagc gcacgctcat cagcgacgac
      661 gccccgttcg accgctacgc cgccggtgat ttcgacgcgc tgaccacgca gcagcggcgc
      721 ggcctggccc tgttccgctc cggcgcgctg cgctgcttcg aatgccacgg cgcgccgacc
      781 ttcgccagcg acacattccg cgtcatcggc gtcgacagcg acgaccccgg ccgcgcggcc
      841 gtcgtggacg agggtctctt cggcgcgttc aaggtcccca ccctgcgcaa catcgccctg
      901 agcgccccct acatgcacga cgggtcgatg gctacgctgg aagaggtggt caagttctat
      961 gagggcggcg gcggccgggc caccggtttc gagcaggtgg acgtgttcgt caacccgttc
     1021 gagctgtcgg atcaggatcg cgccgacctg atctccttcc tctatgccct gaccgacgag
     1081 agcaacaagc cggagattcc cgcggccgtg ccgtcgggtc tggcggtggt tgcgccctac
     1141 gacaaccccg cccgcgccga agtggcgctg cacaacacgg ccgccggtgg cgcggtggtc
     1201 gaggacgggc cgccgcagac catcaccgtg cagccgggcg agaccatcca gtcggccgtc
     1261 gaccgcgcgc gccccggcga cacggtggag atcccctacg gcgtctatca tgagcgcgtc
     1321 gtcatcgaca tgagcgactt cacgctggtc ggcgtgccca acgaggcggg cgagttcccc
     1381 atcctcgacg gcgagggcaa gctgccggaa ggggtcatcg ccagcggcaa caatttcagc
     1441 gtgggcaatc tccacacgcg caactacacc gacaacggcg tcctcgtcga gggcgtgacc
     1501 ggcgtctatt tccacgacat cttcgccgag aatgtgggca cctacggcgt ctatcccgtg
     1561 cgcagcaccg acgtgcttat cgagcgggtg acggtgaccg gcgtcgacga cgcgggcatc
     1621 tatgccgggc agagcgagaa cgtcaccgtg cgcgattcgg tcgcctacgg caacgtcatc
     1681 ggcatcgagc tggagaacac gctgggcggc gagctgtata acaaccatct ctatgacaac
     1741 agcaacggca tcctgctggt gctgctgccg cagctgtcgt cgaagatctc ttccggggcc
     1801 atcgtccgcg acaatctggt ggagaacaac aaccatccca acttcgcgcc cgaagggggc
     1861 atggcccgca ttgtgccgcc gggaacgggc atcctgatca tcggctccga caacaacgag
     1921 gtgttcaaca acaccatcac cggcaacaag acggccggca tcgccgtgtt cagtctgacg
     1981 ggcaccggcg cgttcaacga gaacgagctg gacgtcggcc cgctggcgga gaacaacttc
     2041 acccacaaca acacgttcag caacaacggc ttcgaccccg acccgacggt gtccgagctg
     2101 ggcatcccgg cgggcgacat cctctgggac acgaccgggg cgaacaatcg cttcaacgag
     2161 agcggggtca gcggcttccc gccgctgctg cccggcgaca actggcccgg cttcgttcgc
     2221 aacgcctacg gcaatttctg gcgggtgctg ctgaaggtga tgggctagaa caaatgacga
     2281 aggacgaagt acgaagtacg aaagaagagc gcgcctcttt cgtacttcat acctcgtact
     2341 ttggaatgac gagtgacgag ggcgctcttg ctgtggtccg tgatcgcgcg gcaggagtcg
     2401 cgtcggtcgt ccgtcggcgg acaggagtcc gctcagcggt cggcggccgt ccgtcgacgg
     2461 tcggcgaacc gcgccgccac aaacacgatt agcatcccca acgccagcca gcccagcagc
     2521 gagtagagcg cggcgaagcc gatggcctca taggccgggc cgccgaggaa gccgcccagc
     2581 gccgccccca gcccgaacat catgctggag aacagcgcct gcgccgtggc ccccagtccc
     2641 cgtggcgcgg ccgcgtcggc atcggccacc cccgccgccc acagtacggc gaacgacggc
     2701 ccgtggagca ggctgatggg cagcaccagc catgggtcgg tgacgatccc catcgccagg
     2761 ttgcgcacca cggtgaacgc cagcgccagg gcgatcatct tgctgctgcc gaaccgccgc
     2821 agcagcccgg cgctgatgaa caggaacggg atctcgctga gggtgttggc ggtcatcgtc
     2881 caggccatga gcgtccggct gccgcccagc tccgcgatat agagaaactg gtagctgagc
     2941 atgaccccca gagtgacgcc gaagaccagc gagacgagca gcagcagcag aaagcggccg
     3001 ttgcgcagca gcacacccag cccggccgaa taactgtgcc gcgcccgttc gccgatgtcc
     3061 atcggcaacc gcgtggcggc gaggaaggtg acggccataa tggccatgaa gccgtagaag
     3121 ccgatggcca ggcccgaccg ctcgatgagc gagccgatga tgagaccggt tgtcccccag
     3181 ccgacgctgc cccacagccg cacgcggccg tagtcgctct tgcggtcgcc caggaaggcc
     3241 atgacggcat tgtcgaccag cggcacgatc ggcccgacaa agaaggcgta gacgatcacg
     3301 gccatgagca gttcgacgaa ggtgtcggcc agcagcagca cgacggccga gccgccgagc
     3361 ccggcgatag tggtcagcag caccgcgcga tggcggcggg tcgcgtcggc gatgccgccc
     3421 cagaacggcg cgctgaacag gttgaccagc gggatggtgc ccgacagcac gccgatttgt
     3481 gccccggtca tgcccagctc ggcataatag agggccatga acggcgccag acaccccagc
     3541 gcggcatagt agaagaagta gaccgatttt ggacgcatgg gaaaattacg aattacgaat
//
LOCUS       DAOMQG010000352         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10634_length_3594_cov_4.424979, whole genome shotgun sequence.
ACCESSION   DAOMQG010000352 DAOMQG010000000
VERSION     DAOMQG010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10634_length_3594_cov_4.424979"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLC06_14390"
     CDS             <1..611
                     /locus_tag="PLC06_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91683.1"
                     /translation="IGSWERIYFSVATAQNLPGMGRVTPQDILIYEPPTSGTVTAGTL
                     QLWMDGSDMGLTTASESIDALAVDYETTAYISTRGSAKLPGDGGSVTYKNNDLIQFDL
                     WTTGQQTDGWSRLLVAGTQLGLGAANVTAMDVIGSSQYYLMFDRPVTLDGIQFAPGDV
                     ANCWFDYWNNGCSYAEKAFDGRALGLGSRKINAITIGDIELH"
     gene            complement(676..2841)
                     /locus_tag="PLC06_14395"
     CDS             complement(676..2841)
                     /locus_tag="PLC06_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRO91684.1"
                     /translation="MSQPTDPTLVPFFDPAGIAIIGASTAPAKLGFGVARNLAQSGYT
                     GAIHFVNPRGGRLLGRPIHPTIAGVPDPVDLAVLIVPAPQVPQALAEAGRRGIRAAII
                     ASGGFRETGPEGALLEEECLRVAHEHAIRLIGPNCVGLIDTHLPLDTTFLPPPGPTPG
                     DIAFISHSGAICAAVIDWARGQGFGLSHLISLGNQIDVGETDVLAPMAADPDTKVITL
                     YLEGIRDGRRFVRAAREVTREKPIIAVKVGRYEAGRRAVAGHTGALAGQESAFNAAFR
                     RAGVIRANTVEELFDWARTLAWCPLPAGPNVAVLTNAGGPGVTAADALESLGLRAAAL
                     SPETVAALRAILPANAGLHNPVDMLAAATAGQYVDSLRILLDDPGVDSVMVILPPPPM
                     ESAGGVARAIIPIILAATKPVVIALMGERMIQEAVEYFRAARVPEYRFPERAAAALAV
                     LTERAAKVRAAAGEIERPRPAGIHPDEARRILDDRRASNATAADAFLPYEVVCELLAA
                     YGIPTPAAELAATAAEAVDIAWRVGFPVVLKVASPDIVHKSEVGGVLLNLQSPDEVKA
                     GFAAVTANAQAAEPAARLDGVHVQHMITSGQEVIIGAVRDPQFETIVMFGSGGVEVEG
                     LRDVEFSLAPLNEAEADVLIDGTWAGRRLAGFRNLPPADRAAVVELIRRLGQLAADFP
                     ELAEIELNPVRVLGVGEGAIAVDVRARLDVRARPASYVQ"
     gene            2991..3584
                     /gene="pyrE"
                     /locus_tag="PLC06_14400"
     CDS             2991..3584
                     /gene="pyrE"
                     /locus_tag="PLC06_14400"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRO91685.1"
                     /translation="MTTMESLALTLHDIGAIQFGRFRLHSGRESRIYIDLRVLVSFPY
                     ALRQATAAYRTVLDGLAFDLLVATPLAGLPIGTALCLDTDKPLIYPRKTAKSYGTGKN
                     IEGRYAIGQTAVVVDDLITSGDSILEAIATVKAAGLKVHDAVVLIDREQGGRAMLEEQ
                     GYRLHAVVAIRDLLATLHEHGRLSRYQHDEVLASLGL"
BASE COUNT          593 a         1162 c         1251 g          588 t
ORIGIN      
        1 atatcggttc ctgggagagg atctatttca gcgtcgccac agcccaaaac ttgccgggga
       61 tgggccgtgt gactccgcag gatatcctca tctacgaacc gcccacaagc ggcacagtca
      121 cggcaggtac cctccagttg tggatggacg gttccgatat ggggctgacc accgcgagcg
      181 aaagcatcga tgcgctggcg gtcgactatg agacaacggc gtacatcagc accaggggca
      241 gcgcgaaact gcccggcgat ggcggctcgg tcacctataa aaacaatgat ctgattcaat
      301 tcgatctatg gacgacaggg cagcagacag acggctggtc gcggttgctg gtcgccggaa
      361 cccaactggg tctcggcgcc gccaacgtca cggcgatgga cgtgatcggg tcctcgcaat
      421 attacctgat gttcgatcgt ccggtaacgc tcgacggtat tcagtttgct cccggcgacg
      481 tagccaattg ctggtttgat tattggaata acggttgcag ctacgccgaa aaggctttcg
      541 atgggcgcgc gcttgggtta ggttcgcgca agatcaatgc tatcaccatc ggagacatcg
      601 aattgcacta acgttcatcc ctgttcagga ttgagtgttt attgggtcac ccgcgggctt
      661 cggctcgcgg gtgattcatt gaacataact tgccggccgc gcccgcacgt ccagtcgcgc
      721 ccgcacatcc accgcaatcg ctccctcgcc gacccccagc acccgcaccg ggtttagctc
      781 gatctcggcc agctcgggga aatcggccgc cagctgcccc aggcggcgaa tgagttcgac
      841 gacggccgcg cggtcggcgg gcggcaggtt gcggaaaccg gccagccgtc gcccggccca
      901 cgtcccgtcg atgagcacgt cggcctcggc ctcgttcagc ggcgcgagcg agaactccac
      961 gtcgcgcagc ccctccacct ccaccccgcc ggagccgaac atgacgatcg tctcaaactg
     1021 cgggtcgcgc accgcgccga tgatcacctc ctgcccgctg gtgatcatgt gctgcacgtg
     1081 gacgccgtcg agacgggccg ccggttcggc cgcctgcgcg ttggccgtca ctgcggcgaa
     1141 gcccgccttc acctcgtcgg gcgattgcag attgagcagc acgccgccca cctcggactt
     1201 gtggacgatg tcgggcgagg cgaccttgag caccacgggg aagcccaccc gccaggctat
     1261 atcgaccgcc tcggccgccg tcgcggccaa ctctgccgcc ggggtgggga tgccataggc
     1321 ggccagcagc tcgcagacga cctcgtaggg cagaaaggca tcggccgcgg tggcgttgct
     1381 cgcgcgccgg tcgtcgagga tgcggcgagc ctcgtcgggg tggataccgg cgggccgcgg
     1441 ccgttcgatc tcgcccgcgg cggcgcgcac cttcgccgct cgttcggtca gcacggccag
     1501 cgcggcggcg gcccgctccg ggaagcgata ctccggcacg cgggcggcgc ggaaatattc
     1561 caccgcttcc tggatcatcc gctcgcccat gagggcgatg accaccggct tggtggcggc
     1621 gaggatgatg gggatgatgg cgcgggccac gccgccggcc gactccatcg gcggcggcgg
     1681 caggatgacc atgacgctgt cgacgccggg gtcgtcgagc aggatgcgca gcgaatcgac
     1741 gtattgcccg gcggtggccg cggccagcat gtcgaccggg ttgtggagtc cggcgttggc
     1801 gggcaggatg gcccgcagcg cggccaccgt ctccgggctg agggcggcgg cacgcagacc
     1861 gaggctctcc agcgcgtcgg cggccgttac gcccgggccg ccggcgttgg tcagcacggc
     1921 cacatttggc ccggcgggca acgggcacca ggccagcgtc ctggcccaat cgaacagctc
     1981 ctcgacggtg ttggcgcgga tgaccccggc acgacggaag gcggcgttga aggccgactc
     2041 ctgcccggcc agcgccccgg tgtggccggc cacggcgcga cgaccggcct catagcggcc
     2101 gaccttgacg gcgatgatcg gcttctcgcg ggtcacctcg cgggcggcgc ggacgaagcg
     2161 gcggccgtcg cggatgcctt ccagatagag ggtgatcact ttggtgtcgg ggtcggcggc
     2221 catgggggcc agcacgtcgg tttcgcccac gtcgatctgg ttgccgaggc tgatgagatg
     2281 ggacaggccg aacccctggc cgcgcgccca gtcgatgacg gcggcgcaga tggccccgga
     2341 gtgggagatg aaggcgatgt cgcccggcgt ggggccgggc ggcggcagaa aggtggtgtc
     2401 caatggcagg tgggtgtcga tgaggccgac gcagttgggg ccgatgagcc ggatggcgtg
     2461 ctcatgggcc acgcgcaggc attcttcttc cagcagcgcc ccttccggcc ccgtctcgcg
     2521 gaagccgccg gaggcgatga tggcggcgcg aatgccgcgg cggccggcct cggccagcgc
     2581 ctggggcacc tggggagcag gcacgatgag cacggccagg tcgaccgggt cgggcacgcc
     2641 ggcgatcgtc gggtggatgg ggcggcccag caaacggccg ccgcgcgggt tcacgaaatg
     2701 gatcgcgccg gtgtagccgc tctgggccag gttgcgcgcc acgccgaagc cgagcttggc
     2761 cggggccgtg gacgcgccga tgatggcgat gccggcggga tcgaaaaagg gtacgagtgt
     2821 gggatcggtt ggttggctca cgacccccta ttgtagacgg ttgtggcgga aaggcaggct
     2881 taatgacgaa ttacgaatta cgaatgaaga attcgtcact cgtcactcgt atttcgtatt
     2941 tcgtatttcg tagttcgtca ctcgtcatta tattgctaga atagggttcc atgaccacca
     3001 tggaatcgct ggctctcacg ctccacgaca tcggcgcgat ccagttcggc cgctttcggt
     3061 tgcacagcgg ccgcgaatcg cgcatctaca tcgatctgcg cgtgctggtg tcgtttccct
     3121 acgcgctgcg gcaggcgacg gccgcctatc ggacggtgct cgacgggctg gcgttcgacc
     3181 tgctggtggc cacgccgctg gccgggctgc ccatcggcac ggcgctgtgc ctcgacacgg
     3241 acaagccgct catctaccca cgcaagacgg ccaagagcta tggcacgggc aagaacatcg
     3301 aagggcgcta cgccatcggc cagacggcgg tggtggtcga cgacctcatc acatcggggg
     3361 acagcatcct ggaagccatc gccacggtca aggcggccgg gctgaaggtt cacgacgccg
     3421 tggtgctgat cgaccgggag cagggcggcc gcgccatgct ggaggagcag ggctaccggc
     3481 tccacgccgt cgtggccatc cgcgatctgc tggccacgct ccacgaacac ggccgcctca
     3541 gccgctatca gcatgatgag gtgctggcgt cgctcggcct gtagcacgac ctgt
//
LOCUS       DAOMQG010000353         3572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10726_length_3572_cov_5.542792, whole genome shotgun sequence.
ACCESSION   DAOMQG010000353 DAOMQG010000000
VERSION     DAOMQG010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10726_length_3572_cov_5.542792"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1026)
                     /locus_tag="PLC06_14405"
     CDS             complement(55..1026)
                     /locus_tag="PLC06_14405"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HRO91686.1"
                     /translation="MSLTIGKMRRLQQCSTPDGLFVIVALDHREDLRRLLKPIEPDSL
                     SYGEMVAFKSEVVQSLSGVVSAVLLDPEYGAAQAIAGGSLPGNTGLLVTIEATGYTGE
                     ATNRRTTLLEGWSVEKIARMGASGVKMRLFYHPEAKSAARQEALVDEVAAACRAADIP
                     LFLEPLSYSPVKGKALSSAEKRAVVIETARRLSMRGIDVLKAEFPLDTAEEPRRGEWE
                     AACAELTEASAVPWVLLSAGVSFKEFRRQTEIACRCGAGGVVVGRAVWQEATELRNKA
                     HDDFLAKTAVERMVELGDLIAEYGTPWTKYHPNPADEVGEGWFARYE"
     gene            complement(1571..3319)
                     /locus_tag="PLC06_14410"
     CDS             complement(1571..3319)
                     /locus_tag="PLC06_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HRO91687.1"
                     /translation="MFARYIITILPTGETLPHPVADESGTLRLELPGRSVTIETPDAI
                     PDTALVIQRGPDQITITEGYPIPATTGGLPHSGIYNEFIIDAAEARAHNDTFGLLACY
                     YARDGRALRLSNSLRLLRRGAGLGTDELGVAEMFLFGGWTPSERIIVRDAWRLAAGTE
                     YRFPLDGRPHTATRLTRTWTSVIDEPVNRVVDRVCDLWNTALESHFDPIDGPIGLMLS
                     GGLDSRMVAGAAAARGKQLVGLTFGDLRSDEVKIAGRVAETTGAKWLPSGLDADFAFE
                     RLAFDRVNRVHETMYNLMWDANAPLLAAEGVAHFSTGATFETVFGGQKDADKRRRFLL
                     NLRQSLLGAWKVPAATAAERVAVAEAIKGIGRKRARHYAPLLAEPYRSLVADNLAAAE
                     REVDERVALIAATGPITAAQIRERFDSEHYQTHYSRNQERQLFTYGTVVLPTIDRDLA
                     NYLTNLPAGMKYDHALYYRVIRRLYPDLAALPVPNLGTGVNKSQLRIELNRVWQIQRK
                     QRLTTWVNFSDWMRLDDNMAKYERLFLEQGHFFDGDAIRAFFTDVRAGRRSIYDGGET
                     TGFLNLAYLQDERRMA"
BASE COUNT          634 a         1120 c         1166 g          652 t
ORIGIN      
        1 tctcgtatct cgtatctcgt atctcgtatc tcgtatctcg tatctcgtat ctcgtcactc
       61 gtatctggca aaccacccct cccccacttc atcggccggg ttgggatgat actttgtcca
      121 gggggtgccg tattcggcga tgaggtcgcc cagctcgacc atccgctcga cggccgtctt
      181 ggccaggaaa tcgtcgtggg ccttgttgcg caattccgtc gcctcctgcc acacggcgcg
      241 gccgacgacc accccgcccg ccccacagcg acaggcgatc tccgtctggc ggcgaaactc
      301 cttgaagctg acgccggcgc tgagcagcac ccagggcacg gcgctggcct cggtcagctc
      361 ggcgcaggcc gcctcccact cgccgcggcg cggttcctcg gccgtgtcca gcgggaactc
      421 cgccttcagc acatcgatcc cccgcatcga gaggcggcgc gccgtctcga tgaccaccgc
      481 ccgcttctcg gccgacgaca acgccttgcc cttgaccggc gaataggaca gcggctccag
      541 aaacagaggg atgtcggcgg cgcggcaggc ggcggcgacc tcatcgacca gcgcctcctg
      601 cctggcggcg cttttggctt cggggtgata gaagaggcgc atcttcaccc ccgacgcgcc
      661 catgcgggcg atcttttcca cgctccagcc ttccagcaag gtcgtgcgcc ggttggtggc
      721 ctcgcccgtg tagccggtcg cctcaatcgt caccagcagg ccggtattgc cgggcagcga
      781 gccgccggcg atggcctggg ccgcgccgta ttccgggtcc agcagcacgg ccgacaccac
      841 gccggacagc gattgcacca cctcggattt gaaggcgacc atctcgccat agctgaggct
      901 gtcgggttca atgggcttga gaaggcggcg caggtcctcg cgatgatcga gggcgacgat
      961 gacgaacagg ccgtcgggcg tgctgcattg ctgcaaccgg cgcattttgc cgatggtcaa
     1021 actcataata actcctgaaa atacgtggaa cgtagcccgg cagccccgac gcgtggcgcg
     1081 gccgatgcgg ctcccaggta attcaacggc cgtatgaacg gtttggtttg tagttagcgg
     1141 ctttagccgc gttccggagc ggcgctaaag cacctactac gaacggtttg tagattttcc
     1201 aggtaataaa tgtaatgtat tcgcggcccc aggctcccat ccggcggagc ggccgcggtc
     1261 ctgctgatat ccggttttat ctgggccagt ttgcggtaat acggctattt gcggatgaaa
     1321 tcggacgatc ccaaaacccg atgaggcaaa ttatcctcgg cctatagcga ccgtgcaaca
     1381 attgggagtg tatttttaat gttggggggc gcacaaccga cgacggacga caagagaacg
     1441 gcgagtgacg actcctgtcg tccgacaaga tgacgagcga cgagtgacga gtaaaggcga
     1501 agtacaaagt acgaaaggcg cgctcttctt tcgtactttg tacttcgtat ctcgtacatt
     1561 tcttcactcg tcacgccatt cgccgctcgt cctgcaaata ggccagattg aggaagccgg
     1621 tcgtctcgcc gccgtcgtag atggagcggc ggccggcgcg gacatcggtg aagaaagcgc
     1681 ggatggcgtc gccgtcgaag aaatggccct gctccaggaa cagccgctca tacttggcca
     1741 tattgtcgtc cagtcgcatc cagtcggaga agttgaccca cgtcgtcagg cgctgcttgc
     1801 gctggatctg ccagacgcga ttcagctcga tgcgcaattg tgacttgttg acgccggtgc
     1861 ccaggttcgg cacggggaga gccgccagat cgggatagag gcgacgaata acgcgatagt
     1921 agagcgcgtg gtcatacttc atccccgccg gcaggttggt cagatagttg gccaggtcgc
     1981 ggtcgatggt cggcagcacc accgtgccgt aggtgaaaag ctggcgctcc tgattgcggc
     2041 tgtagtgggt ctggtaatgc tcgctgtcga accgctcgcg aatctgggcg gcggtgatgg
     2101 ggccggtggc ggcgatgagc gccacccgct cgtccacctc gcgctcggcc gcggccaggt
     2161 tgtcggccac cagtgagcgg tacggctcgg ccagcagcgg ggcataatgg cgggcgcgct
     2221 tgcggccgat ccccttgatc gcctcggcca cggcgacgcg ctcggcggcc gtggccgccg
     2281 gaaccttcca cgccccaagc agcgactggc gcaggttcag cagaaagcgg cggcgcttgt
     2341 cggcgtcctt ctggccgccg aagaccgtct cgaaggtcgc gccggtggag aaatgggcca
     2401 cgccctcggc cgccagcagg ggggcgttgg cgtcccacat caggttatac atcgtctcgt
     2461 ggacgcgatt gacgcggtcg aaggccagcc gctcgaaggc gaagtcggcg tccagcccgc
     2521 tggggagcca ttttgcgccg gtcgtttcgg ccacgcgccc ggcgatcttc acttcatcgc
     2581 tgcgcaaatc gccgaaggtg aggcccacca gctgcttgcc ccgcgccgcg gccgccccgg
     2641 ccaccatgcg cgagtccagc ccgccgctca gcatcaggcc aatcggcccg tcgatggggt
     2701 cgaaatggga ctccagcgcg gtgttccaca ggtcgcagac gcggtcgact acccggttca
     2761 ccggctcgtc gatgaccgac gtccacgtcc gggtcaggcg ggtggcggtg tgggggcggc
     2821 cgtccagcgg gaagcgatac tccgtgcccg ccgccagccg ccaggcgtcg cggacgatga
     2881 tccgctcgct gggcgtccag ccgccaaaga ggaacatctc ggccacgccc agctcgtccg
     2941 tgcccagccc cgcgccgcgc cgcagcaggc gcagcgagtt ggacaggcgc agcgcgcggc
     3001 cgtcgcgggc atagtagcag gccagcaggc cgaaggtgtc gttgtgggcg cgcgcctcgg
     3061 cggcgtcgat gatgaactcg ttgtagatgc ccgaatgggg caggccgccg gtcgttgccg
     3121 ggatggggta gccctcggtg atcgtgatct gatccggccc acgctggata accagcgcgg
     3181 tatccggtat ggcgtcgggc gtctcgatgg tgacggaacg gccgggcagc tccagccgga
     3241 gcgtgccgct ttcgtcggcg acgggatggg gtaacgtctc cccggtgggc aggatggtga
     3301 tgatatagcg cgcgaacacg gataatcccc ctggactaaa ggatttatga cgaactgagg
     3361 cccatgttaa caaaatcggg tcgcgtctac catcgataaa tcgtgaaaag tggtctgtgc
     3421 gggttaagtg agagactcgt ccgtttattt gcgcgcggcg cgaaaatttg tcaatatacc
     3481 ggtcgatttt gtgccggtaa tcccggactt atttgaggag acaacatcat gggttcagca
     3541 cggagccgga agaagccggc gaacaagaaa gc
//
LOCUS       DAOMQG010000354         3525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10899_length_3525_cov_10.599712, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000354 DAOMQG010000000
VERSION     DAOMQG010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10899_length_3525_cov_10.599712"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLC06_14415"
     CDS             <1..236
                     /locus_tag="PLC06_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91688.1"
                     /translation="VLIGVVTSVISAYYYLRIVFYMYMYEGEGEMILKRPLVTVVAIT
                     ALATLLLGILPGTWFDLARQAVIQSVQAVAAGG"
     gene            325..819
                     /locus_tag="PLC06_14420"
     CDS             325..819
                     /locus_tag="PLC06_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HRO91689.1"
                     /translation="MIHMIVFAMPSPEGAYEMRNALDDLRAFDLVDVKDAAVVEHCLD
                     GSVRVRQAFDLIGAGALGGAVWGALIGLIFLSPWLGMAAGGLAGALAGKFTDIGIDDD
                     FIREVGENVKPGHSALFMLIDNWENSKSLRLLADFDAVIIHTTMPADEEARLRAFFIA
                     AAND"
     gene            975..3443
                     /locus_tag="PLC06_14425"
     CDS             975..3443
                     /locus_tag="PLC06_14425"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRO91690.1"
                     /translation="MDFKQHPTTNRTSREPDVLRQNIVDNLYYSRGANVQSAGDYDIY
                     MALSRSVRNHLVERYRRTVDMRYAVNPRFVYYLSAEYLLGRQLPQNMLYTDTTDIARK
                     ALDGSQFPLDEVAPHDVEPGLGNGGLGRLAACFLDSLATLDIPAIGYGIRYEYGIFRQ
                     EIRDGYQVEQPDNWLALDYPWEFAQPDDMIPIGFGGHVEHYQDENGRYRVRWIPGEQV
                     MGEPHHVLVPGYGTTTVNFLRLWRARATSEFDFRLFDIGDYASAVEAKVHSETISKVL
                     YPNDNTPQGRELRLRQQYFFVACSLHDIIARFLRCGNVIEDLAEKVVIQLNDTHPVVG
                     IPELMRVLIDDYNMEWHEAWEITTHTFAYTCHTLMPEALEKWPVSLFERLLPRHMEII
                     NEINRRFLAEVSARFPNDYDRLSRMSLIQDGGDRQVRMAHLAVVGGFSVNGVADLHSQ
                     LLKERVLKDFYEMWPEKFSNKTNGVTPRRFMRLANPRLSALITEKLGEGWLTDLDRLK
                     KLEDYVDDPDFRAKWRAVKAANKVDLAAHIAKKTGLVVDPNSMYDVMVKRLHEYKRQL
                     LDALHIITLYNRLKADPTLDIVPRTFIFGAKAAPGYFMAKLIIKLINSIGAAVDDDPV
                     VSKLLKVVFLPNFNVTQGEIIYPAADLSEQISLAGKEASGTGNMKFALNGALTIGTLD
                     GANIEIRDRVGAENFFLFGLKVDEVFALKAKGYNPRTYVAADPELKKAIDQISSGFFS
                     GGDPTLFQPIIQSLLDRDEFLVLADYRAYVDCQDEVEKVYRDSEAWTRKSILNAARCG
                     YFSSDRAIREYAEGIWKVKSLPVP"
BASE COUNT          681 a         1071 c         1037 g          736 t
ORIGIN      
        1 tggtgctcat cggcgtcgtc accagcgtca tctccgccta ttactacctg cgcatcgtct
       61 tctatatgta catgtatgag ggcgaaggcg aaatgatcct gaagcggccg cttgtaacag
      121 tcgtggccat caccgccctg gcgactctgc tgctgggcat tctgcccggc acatggttcg
      181 atctggcccg tcaggcggtc atccagagcg tgcaggctgt cgccgccggc ggctaatcaa
      241 tcaggaatta cgaattacga attacgaatg aagaggcgct ctctttattc gtaattcgta
      301 atccgtaatc cgtaattcct tcccatgatt catatgatcg tctttgccat gccctcgcct
      361 gagggcgcgt atgagatgcg caatgcgctg gacgatctgc gcgccttcga tctggtcgat
      421 gtgaaggacg cggccgtggt ggagcattgc ctcgacggct cggtgcgcgt gcggcaggcg
      481 tttgacctga tcggcgcggg tgcgttgggc ggggcggtct ggggcgcgct catcgggctg
      541 atcttcctgt cgccatggct ggggatggcc gcgggcgggc tggccggcgc gctggccggg
      601 aagttcaccg atatcggcat cgacgacgac ttcattcggg aagtcggcga gaacgttaaa
      661 cccggtcatt ccgccctttt catgctgatc gataactggg aaaatagcaa gtcattgcga
      721 ctcctggctg atttcgatgc cgttattatc cacaccacca tgccggccga tgaggaagcc
      781 cgattgcggg catttttcat cgcggcggcc aatgattagt cattattcac cctgcccttc
      841 ataacgcaat tcctgccggg ttgccggctg cttcggtgcg gcgagcttcg cgacgcgccc
      901 atatcggccc ttgccgaaaa cttctaacga cgggagacgg gtcaatggcc cttccccacg
      961 agcggagagc ttgaatggat ttcaaacaac accccaccac caatcgcacc agccgcgagc
     1021 cggatgtgct ccggcagaac atcgtcgata acctttacta ttcgcgcggg gccaacgtgc
     1081 aatcggccgg cgattacgac atctacatgg cgctgtcgcg cagcgtgcgc aatcatctcg
     1141 tggagcgcta tcgccgcacc gtggatatgc gctacgccgt caacccgcgc tttgtctact
     1201 acctatcggc cgaatatctg cttgggcgcc aattacccca gaacatgctc tacaccgaca
     1261 caaccgatat cgcccgcaag gcgctggacg ggtcacagtt ccctctggac gaggtcgcgc
     1321 cccatgacgt ggagccgggg ctgggcaacg gcggtttggg tcgactggcg gcttgctttc
     1381 tcgattcgct ggccacgctc gacatcccgg ccatcggcta cgggatacgc tacgaatatg
     1441 gcatctttcg acaggagatc cgcgacggct accaggtgga gcaacccgac aattggctgg
     1501 ccctcgatta cccgtgggag ttcgcccagc ccgacgacat gatccccatc ggcttcggcg
     1561 gccatgtgga gcattatcag gacgagaacg gccgctatcg ggtgcgctgg atccccggag
     1621 agcaggtcat gggtgagcca catcacgtgc ttgtgcccgg ctatggcacg acgacggtca
     1681 acttcctgcg cctctggcgg gcgcgagcca cgtcggagtt tgacttccgt ctgttcgata
     1741 tcggcgacta tgccagcgcc gtggaagcca aggttcacag cgagaccatc tccaaggtgc
     1801 tctatcccaa cgacaatacc ccccaggggc gggaactgcg cctgcgacag caatatttct
     1861 tcgtcgcctg ctcgctgcat gacatcatcg ctcgcttcct gcgctgcggg aacgtgatcg
     1921 aggatttggc cgagaaggtg gtcatccagc tcaacgacac ccatccggtc gtcggcatcc
     1981 ccgaattgat gcgcgttctg atcgatgatt acaatatgga atggcatgag gcatgggaga
     2041 tcaccaccca cactttcgcc tacacctgcc acacgctgat gcccgaagcg ctggagaaat
     2101 ggccggtcag cctgttcgag cggctgctgc cccgccacat ggagatcatc aacgagatca
     2161 accgccgctt cctggccgag gtcagcgccc gctttcccaa tgattacgat cgcctgtcgc
     2221 gcatgtcgct cattcaggac ggcggcgacc gccaggtgcg catggctcat ttggcggtgg
     2281 tcggcggctt ctcggtcaac ggcgtggccg atctgcattc gcaactgctc aaggagcgcg
     2341 tgttgaagga cttctacgag atgtggccgg aaaagttcag caacaaaacc aacggcgtca
     2401 cgccccggcg cttcatgcgt ctggccaatc cgcgcctgtc ggcgttgatc accgaaaagc
     2461 tgggcgaggg ctggctgacc gatctggatc gcctgaagaa gctggaagat tacgtcgatg
     2521 atcccgactt tcgggccaaa tggcgcgcgg tgaaggcggc caacaaggtc gacctcgcgg
     2581 cccatattgc caaaaagacc ggcctcgtcg tcgacccgaa ctcgatgtat gacgtgatgg
     2641 tcaagcgctt gcacgagtac aagcggcagc tgctggatgc actccacatc atcaccctct
     2701 acaatcgcct gaaggccgac ccgacgctcg atatcgtccc gcgcaccttc atcttcgggg
     2761 ccaaggccgc gcccggctac ttcatggcca agctcatcat caagctcatc aactccattg
     2821 gcgcggccgt cgatgacgat ccggtggtga gcaagctgtt gaaggttgtc ttcctgccca
     2881 attttaatgt gacgcaaggg gagatcatct accccgcggc cgacctgtcg gagcagatct
     2941 cactggcggg caaggaagcg tcgggcacgg gcaacatgaa gttcgccctc aacggcgcgc
     3001 tgaccatcgg cacgctcgac ggagccaaca tcgagattcg cgatcgggtt ggcgcggaga
     3061 acttcttcct gttcggcctg aaggttgacg aggtgtttgc tctgaaggcc aagggctata
     3121 accctcgtac ctacgtggcc gccgatcccg agctgaagaa ggcgatcgac cagatcagct
     3181 ccggcttttt ctccggcggt gacccgacgc tctttcagcc gatcatccag tcgctgctcg
     3241 accgcgacga gttcctggtg ctggccgatt accgcgccta tgttgattgt caggacgagg
     3301 tggagaaggt ctatcgggac agcgaggcct ggacgcgcaa gtcgatcctc aacgcggcgc
     3361 gctgcggcta tttctcgtcc gatcgggcca tacgcgagta tgccgagggc atctggaaag
     3421 tgaagtcgct gccggtgccg taaagactgg gtatttttgc ctagcaagca ggggcgctca
     3481 tttgcgtctc attttatact tgattatact tgctctatgg tccga
//
LOCUS       DAOMQG010000355         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_10952_length_3509_cov_7.060799, whole genome shotgun sequence.
ACCESSION   DAOMQG010000355 DAOMQG010000000
VERSION     DAOMQG010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10952_length_3509_cov_7.060799"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLC06_14430"
     CDS             complement(<1..258)
                     /locus_tag="PLC06_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91691.1"
                     /translation="MEACATFVLGSETGVKFSCFVNRLIRALMVLEQVDLSGEDSDLL
                     IDLIAMSRDDATPVARLVTRHSLLSVPLTDGSRPQRNGRSRS"
     gene            411..1472
                     /locus_tag="PLC06_14435"
     CDS             411..1472
                     /locus_tag="PLC06_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRO91692.1"
                     /translation="MNELKLEGLSRRNFLKFAGLTGVSVGLAACAIEPESEIAAQQPT
                     ATPAAEHTMEPAPTASAAEVAAEMDRMHEEGVKIFVDGAGSNPGFWGVPLEHTMDGDT
                     KVFELTCAETKWEVEPGRTIDALTYNGILPGPVIRVTEGDNVRVIVHNEMTQSTSVHW
                     HGVLLANDQDGVPYLTQPPIRPGESFTYEFLTRNSGSHMYHSHHNAAEQVTRGLLGAF
                     IIDPKDPSADPAHDSDYTLILNDTVTGFSINGKGFPYTQPIVAKKGEKLRIRYMNEGL
                     TIHPMHLHGLEQLVFAKDGWNLSSPYYCDTLNIAPGERYDVIVDCHTTGVWAFHCHIL
                     THAESAHGMFGMVTAVIVN"
     gene            complement(1697..2179)
                     /locus_tag="PLC06_14440"
     CDS             complement(1697..2179)
                     /locus_tag="PLC06_14440"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HRO91693.1"
                     /translation="MDDKTRALVALRAVFNQWKSLLAGLREPELTAPLIGELSLKDEV
                     AHLRAWLVVSIARLEAAQSGRAPVYPAWASGLLSADDEPATDRMNREIYDVWRARPWV
                     EVYRTWRDDYLRFLALAEAVPAGDLTDPARCPWLNGHALIDVLWGCEGHHREHLAELI
                     "
     gene            2303..3292
                     /locus_tag="PLC06_14445"
     CDS             2303..3292
                     /locus_tag="PLC06_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91694.1"
                     /translation="MRNRLFPILGRIGFALLSAAVLIVFSEKMFWYVTGYSFLDLLIG
                     YFFPAFILLWVIDAFRVRRLAPLFLAGAVFGFLAEGMLVGTIYEGGPLNWFSVSYTPL
                     AWHAPLSVVFGWYALRRWLVAGRPRAMALGCAAVGLFWGLWAMAWWLPENATDPVLLA
                     QGARLGQWPVGEFALHAFTFSGVLAVALWLLGRGGWSSFRPSKVEVAVVVAGLLVFFV
                     AGVLLAVPWAPLKLLPLLGVTLLALYVNKRKEPPGSLLADVAGPVQLARLPLLLVMPA
                     VAVAVYALGVVLAVPVEVIYGITAVGGVMGTAVLGALVFLAALVWTIRGSRNR"
BASE COUNT          596 a         1081 c         1170 g          662 t
ORIGIN      
        1 cgacctcgac ctgccgttcc tctgcgggcg ggacccgtcg gtgagtggaa cactcaacag
       61 ggagtgacga gtgacgagtc gcgcgacagg agtcgcgtcg tcgcgcgaca tggcgatgag
      121 gtcgatgagc agatcgctgt cttcgccact caaatccacc tgctccagca ccatcagggc
      181 gcgaatgagc cgattgacga agcagctgaa cttgaccccc gtctcgctgc cgagcacgaa
      241 ggtggcgcat gcctccatgg gggatcggcc gggcgcgggg cagttcggcg ggaagtaggt
      301 cgcgccggcg gggatggtct ggccgtcgag tcacttggcg cgtttccccc ttttgcgcca
      361 tacttccagg ttttggaaag caaaatctga tttggagaga gccgattatc atgaatgaat
      421 tgaagctgga aggattatcg cgtcgcaatt tcctgaaatt cgccgggctg accggggtga
      481 gcgtggggtt ggcggcgtgc gccatcgagc cggagtcgga gatcgccgcg caacagccca
      541 cggccacgcc ggcggccgaa cacaccatgg agccggcccc caccgcctcc gcggccgaag
      601 tggcggccga gatggaccgc atgcacgagg aaggggtgaa gatcttcgtc gacggcgcgg
      661 gcagcaaccc cggtttctgg ggcgtgccgc tggagcatac gatggacggc gacacgaagg
      721 tgttcgagct gacctgcgcc gagacgaagt gggaagtgga gccgggccgc accatcgacg
      781 cactgaccta caacggcatc ctgcccggcc cggtcatccg cgtgaccgag ggggacaatg
      841 tgcgcgtcat cgtccacaac gagatgaccc aaagcaccag cgtccattgg cacggcgtgc
      901 tgctggccaa cgatcaggac ggcgtgccct atctgaccca gccgcccatc cgccccggcg
      961 agagcttcac ctatgagttc ctgacccgca attcaggctc ccacatgtac cattcgcacc
     1021 acaacgcggc cgaacaggtg acgcgcggtt tgctgggcgc gttcatcatc gaccccaagg
     1081 acccgtcggc cgacccggcc cacgattccg actatacgct gatcctcaac gacaccgtca
     1141 ccggcttcag catcaacggc aagggtttcc cctataccca gccgatcgtc gccaaaaagg
     1201 gggagaagct gcgcatccgt tatatgaatg aggggctgac catccacccg atgcaccttc
     1261 atgggctgga gcagctggtg ttcgccaagg acggctggaa tctctccagc ccctactatt
     1321 gcgacacgct caacatcgct cccggcgagc gctacgacgt gatcgtcgat tgccacacca
     1381 ccggcgtgtg ggccttccac tgccatatcc tgacccacgc ggagagcgcg cacgggatgt
     1441 tcggcatggt gacggcggtc atcgttaatt aggaattacg aattacgaat tacgaattga
     1501 agagggcggg tctcaattcg tcatttgaac gacaaaaaga aaccgggttt ctgaaaccgg
     1561 gtttcttccg agaaacccgg tttcttgtct taatcaacgg gttgcgaccc gcagctgccc
     1621 gcgcaccgca ccggccgggt agtcggccgt gtggacgttg acgtaaattc gtaattcgta
     1681 attcgtaatt cgtaattcag atcagctccg ccaaatgctc ccgatggtgg ccttcacagc
     1741 cccacaggac gtcgatgagg gcgtggccgt tgagccaggg gcagcgggcg gggtcggtca
     1801 ggtcgccggc ggggacggcc tcggccaggg ccaggaagcg caagtagtcg tcgcgccagg
     1861 tgcggtagac ttcgacccac ggccgcgcgc gccatacgtc gtagatctcg cggttcatgc
     1921 ggtcggtggc cggttcgtca tcggcggaca ggagtccgct tgcccaggcg ggatagaccg
     1981 gcgcgcggcc ggactgcgcc gcttccagcc gggcgatgga caccaccagc cacgcccgca
     2041 aatgggctac ctcatccttg agggacagct cgccgatcag cggcgcggtc aattccggct
     2101 cgcgcagacc ggcgagcaga ctcttccact gattgaacac ggcgcgcaag gcgacgaggg
     2161 cgcgggtttt gtcgtccatg aggggattat agaattacga attacgaatt acgaatctac
     2221 tcgataaaaa ccctgccgcg cccacctgtc aggtctctcc cggtcacgca acttcttttg
     2281 cctcgcgcgc gtacagatgg gcatgagaaa ccggctgttc cccatcctcg gccgcatcgg
     2341 gtttgccctg ttgtcggccg ccgtcctgat tgtgttctcc gagaagatgt tctggtatgt
     2401 caccggctac agcttcctcg acctgctgat cggctacttc ttccccgcgt tcatcctgct
     2461 gtgggtgatc gacgccttcc gcgtgcggcg gctcgcgccg ctgtttctgg ccggggcggt
     2521 gttcggcttt ctggccgagg ggatgctggt gggcacgatc tacgaggggg ggccgctcaa
     2581 ctggttcagc gtcagctata cgccgctggc ctggcacgcg ccgctcagcg tggtgttcgg
     2641 ctggtacgcg ctgcggcgct ggctggtggc cgggcggccg cgggcgatgg cgttgggctg
     2701 cgcggccgtc gggctgttct gggggctgtg ggccatggcc tggtggctgc cggagaacgc
     2761 caccgacccc gtgctgctgg cgcagggggc gcggctgggg caatggccgg tcggcgagtt
     2821 cgcgctccac gccttcacct tcagcggcgt gctggccgtg gccctgtggc tgctcggccg
     2881 cggcgggtgg tcgtcgttcc ggccgtcgaa ggtcgaggtg gcggtcgtgg tcgccgggct
     2941 gctggtgttc ttcgtggccg gggtgctgct cgctgtgccc tgggcgccgc tgaagctgct
     3001 gccgctgctg ggcgtgacgc tgttggcgct ttatgtcaat aaacgaaaag agccgcccgg
     3061 ctccctgctg gccgatgtgg ccgggccggt gcagctcgcg cggctgccgc tgctgttggt
     3121 catgccggcg gtggccgtgg cggtctacgc cctgggggtc gtcctggccg tgccggtcga
     3181 ggtcatctac ggcatcaccg ccgtcggcgg ggtgatgggg acggcggtgc tgggggcgct
     3241 ggtctttttg gcggcgctgg tgtggacaat tcggggaagc cgcaacagat agaaaccagt
     3301 cttctcgaca gaaccataga aaccgggttt ttcggaaaac cccggtttct cccggaaacg
     3361 agataaaagt tcggaggcgc gctccggact cgtatttcgt atccccgggt ggtatactgt
     3421 ttccatgagc gaaaaacagt ccatcgtcgc cgatccggcc gtcatgctgg gcaagcccgt
     3481 cgtggccggc acgcggatca ccgtcgaat
//
LOCUS       DAOMQG010000356         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11109_length_3470_cov_8.526501, whole genome shotgun sequence.
ACCESSION   DAOMQG010000356 DAOMQG010000000
VERSION     DAOMQG010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11109_length_3470_cov_8.526501"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(405..3347)
                     /locus_tag="PLC06_14450"
     CDS             complement(405..3347)
                     /locus_tag="PLC06_14450"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRO91695.1"
                     /translation="MNEPDPSSPDLNKSIFTALIDAFKGLPPILSYGGLVTVIVAFLL
                     VVTGTFDWKLLIIPALVLAAFLIHTFYRDKRHYDLEEKKEGYRHDEVMRPDPLPGPSE
                     TPNPRPTPSVEGDIPVDEWQRRYMAYLARLNSYPPYTALIDIREAGFKETKITLERIY
                     TNLEVPSAEHLRRLPKKAMTDLDSPELEQIGKEQREPVLKAISRPENEHLVILGAPGS
                     GKSTLVSYLALCLARDHLKDEKINQELLREQGWGLSHLRLIPVPVTLRTYAAEGLSKG
                     QDLWTYIEAVLAAANLAGYAPYLKKQLAQGGLLLLDGLDEVDNAPKVRKALKTQVESF
                     VDTFPNVRIIVTSRPYAYGAGWELSRFSVTRLLDFSPEQIDTFIDQWYGATGEDPVLG
                     LGPDKAKAYADSLKAQLNPEYRSTHSLRELASNPLLLTMMVYIHRGREGGQLPHRREE
                     LYRLCINLLLELWQHSKNINLLDELNITREQLEKALQEVAFVAHKSQPATSKTADITG
                     ELLAAKLFKYRGEGAAVDPVTLIQYVRDRAGLLQQHHTKASESDDDLYRFPHRTFQEY
                     LAALYLLRHDFPKELARLSRVDSDRWREALLLAAATQRDVPFSVWALIDKLCPEPPPA
                     SANPSAEAVSGGFLAGRALVETDLLNPETQLDHDEIGKKERVCQWQKTIVTSGLLQPS
                     DRADAGDLLAELGDDRPGVLACDDMRLCYVPPGDFWMTDEPRSKKGHPLDILNKPYWL
                     AQYPVTVAQFREFVHSNPDYKPAYGDMSISSPGNRPVGYVNWYDALAFCEWLNRRWGA
                     YLPPGYRVTLPSEAEWEKAARGGSVVPVDHHATTVHSLKETLATPPVTMPNELPRREY
                     PWGDEPEKPAAADDPYRANNEAAGVGRPTAVGSFPAGGSRLGCLDMSGNVWEWTRSYY
                     DKKRPYRLSAEYETVNPNNRDLMLICGGAYYSDYTGCSARFRYLPHFDFGVDLGFRVA
                     VSPFVSDR"
BASE COUNT          704 a          991 c         1044 g          731 t
ORIGIN      
        1 tcgcctatgt gcgctacgcg atcgtgtatc tgcggctgga cgacctgacg gccattcgcg
       61 gcgagatcga tcggctgctg acggaacaag catagcggcc gcccccaaag cgggcggttt
      121 atcaagcgta tcggtaacct atatttagac gcatccgcgt cattctcacc cctcgaagcc
      181 ctgttctcgc agggcttcgc ttttttgggg ttaaccacgt tcgctacggc aatacgatcg
      241 ggttgcgaaa aaagatgctc ggcgaccatc ccgttccccg cccccaattg ccgccacaac
      301 aggacgagcc acccgacaaa gactagaatt tttgcgccgc cggggggcaa gccccccaga
      361 ccccccagag atcaggtgat catcgggatc agagatcaga gggttcagcg atcagagaca
      421 aatggggaca ccgccacccg aaacccgagg tcgacgccga agtcgaagtg cgggaggtac
      481 ctgaagcggg cggagcagcc ggtgtaatcg ctatagtatg cgccaccaca tataagcatg
      541 agatccctgt tattcgggtt gacagtctcg tattcggccg acaggcgata ggggcgcttc
      601 ttgtcgtagt agctgcgcgt ccattcccag acgttgccgc tcatatccag gcaacccaac
      661 cggctacccc cggccggaaa gctgcccaca gccgtcgggc ggccaacgcc agccgcctcg
      721 ttgttggccc ggtatggatc gtcggccgcc gccggctttt ccggctcgtc gccccaagga
      781 tattcgcgcc gggggagttc gttgggcatc gtgaccggcg gcgtagcaag cgtttccttg
      841 agggaatgaa ccgtcgtggc gtggtgatcg accgggacaa ccgaaccgcc ccgcgccgcc
      901 ttctcccatt ccgcttcgct gggcagggtg acgcggtaac ccggcggcag ataagccccc
      961 cagcgccggt tgagccactc gcaaaaggcc agggcgtcat accagttgac gtagcccacc
     1021 ggccggttac ccggtgatga aatagacatg tcgccatagg ccggtttata atccgggttg
     1081 ctgtgaacaa attcgcggaa ctgggccacg gtgacgggat attgagccag ccagtagggc
     1141 ttgttcagaa tatcgagcgg gtgtcctttt ttgctcctgg gttcgtcggt catccagaag
     1201 tcgccgggcg gcacatagca gaggcgcatg tcgtcgcagg cgagcacgcc ggggcggtcg
     1261 tcgcccagtt cggccagcag gtcgccggcg tcggcgcggt cggacggctg cagcagaccg
     1321 gatgtgacaa tggtcttttg ccattgacac acccgttctt ttttcccgat ttcgtcatgg
     1381 tccaattggg tttccgggtt caacagatcg gtttcgacca atgcccggcc ggcaaggaag
     1441 cctcctgaaa cagcttcagc gctcggattt gccgacgcgg gcggcggttc agggcacaac
     1501 ttgtcgatca aggcccaaac ggagaagggg acatctcgct gggtggcggc ggccagaagc
     1561 agggcttcac gccagcggtc agagtcgacg cggctcaggc gagccagctc tttgggaaaa
     1621 tcgtggcgca gcaaatagag cgcggccagg tattcctgaa aggtgcgatg gggaaagcgg
     1681 tacaggtcgt cgtcgctctc cgaggctttg gtatgatgct gttgcagtaa tccggcccgg
     1741 tcgcgcacat attgaatgag agtcacggga tcgacggccg cgccctctcc ccgatacttg
     1801 aacagtttgg cggccagtag ttcgccggtg atgtcggccg ttttgctcgt ggcgggctgg
     1861 cttttgtgcg cgacgaaggc aacttcctgc aatgcttttt ccagttgttc ccgggtgatg
     1921 ttcagttcgt cgagcagatt gatgttcttg gaatgttgcc acaattcaag cagcagattg
     1981 atgcacaggc gatagagttc ctcgcgccgg tgggggagct ggccgccttc acggccgcga
     2041 tggatgtaga ccatcatggt caacaagagc gggttgctcg ccagttcgcg cagactgtgg
     2101 gtgcttcgat attccgggtt gagctgggct ttcaggctat cggcatacgc cttggccttg
     2161 tccggcccca ggcccagcac ggggtcttcg ccggtcgccc cgtaccattg gtcaataaac
     2221 gtatcgattt gctcggggct gaaatccagc aggcgagtga cggagaagcg gctcagttcc
     2281 cagcccgcgc cataggcata ggggcggctg gtgacgataa tcctgacatt ggggaatgtg
     2341 tcgacaaaac tttcgacctg tgtcttcaac gccttgcgca ccttcggtgc gttatctacc
     2401 tcatccaggc catccaggag aagcaggccg ccttgcgcca actgtttttt caggtatggc
     2461 gcgtagccgg ccaaatttgc cgcggccagg acggcttcaa tatatgtcca gagatcctgt
     2521 ccctttgaga gcccctcggc ggcataggtc ctcaatgtta ccgggacggg tatcaggcga
     2581 agatgggaca ggccccagcc ctgttcccgc aatagctcct gattgatttt ctcatctttc
     2641 agatgatctc tggccaggca aagggccagg taactgacca gggtagattt gccggatccg
     2701 ggcgcgccga gaatgaccag atgttcgttc tccggccggc tgatagcctt cagtaccggc
     2761 tctcgttgct ccttcccgat ttgctccagt tccggactgt ccagatcggt catcgccttt
     2821 ttgggcaacc tgcgcagatg ttcggcagaa ggcacctcca gattagtgta gattcgttcc
     2881 agcgtaattt ttgtttcttt gaaccccgcc tcgcggatat ctatgagagc cgtatagggg
     2941 ggatagctgt tcaatcgcgc cagataagcc atgtagcggc gttgccattc gtcgacggga
     3001 atatcccctt cgaccgaagg agtcggccgc gggttgggtg tttccgacgg accgggtagc
     3061 gggtcagggc gcattacctc atcatgtctg tagccctcct tcttctcctc aagatcataa
     3121 tggcgtttgt ccctgtaaaa agtgtgaatc aggaacgctg ccaaaaccaa agcgggaata
     3181 atgagaagct tccaatcaaa ggtacctgtg acaaccagga ggaaagcaac gattacggtg
     3241 acaagaccgc cataactcag aatcggcgga agccccttaa aggcatcgat cagggcggta
     3301 aatatcgatt tgttgagatc aggactggaa ggatccggct cgttcatggc ggaattatag
     3361 aatagtacgg agaaagtgaa caggagcaac cacctacggc aaccttctcg gactagcaaa
     3421 cggccacgcc cgaaagtcgc ggccgcaatc gtccgtgtcg tcccaatacg
//
LOCUS       DAOMQG010000357         3461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11141_length_3461_cov_8.603347, whole genome shotgun sequence.
ACCESSION   DAOMQG010000357 DAOMQG010000000
VERSION     DAOMQG010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_3461_cov_8.603347"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3461)
                     /gene="gltB"
                     /locus_tag="PLC06_14455"
     CDS             complement(<1..>3461)
                     /gene="gltB"
                     /locus_tag="PLC06_14455"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRO91696.1"
                     /translation="FEHEACGVGFLADIKGRRSHAIIADGLTMLENMSHRGARGADPN
                     TGDGAGILIQIPHNLFSQLTQQLNIHLPPPGAYGVGMVFLPREAARRAECEATLAAIV
                     AAEGQTLLGWRDVPTSNADLGATAVRSEPVMRQVFIGRGQGLSDDPLAFERKLFVIRR
                     LAEKQIGRDASEGDAAFYIASLSSRTIVYKGMLLAEQLRGYFPDLSHPLIDSAIALVH
                     SRFSTNTFPAWKRAHPSRFLAHNGEINTIKGNVNAMRARQALFHSDLFGDDLPKVLPI
                     IDEDGTDSAMFDNALEFLSLAGRSLPHAVMMMIPEPWEKNPEMSAGKRDFYAYHSALM
                     EPWDGPASIVFSDGVVVGAVLDRNGLRPSRYYVTADDRVIMASEVGVLDVPPELIRYK
                     GRLQPGRMLLVDTSEESIVDDDELKQRVAAEHPYGEWLARHMLTLEDLPEGGRERAPG
                     HDTIVLRQHAFGYTFEDLRMILAPMARDGVEPLASMGDDTPIAALSRRPQLLYHYFRQ
                     LFAQVTNPPIDAIREELVTSSAMLLGSEGDLVNPRPEDCRRIRLQSPVLTNAELARLR
                     GIGGHKPYRAGFSAVTLPMLFPSGSGGTGLAAALDKLFATADRGLAGGANLIILSDRG
                     ISRDEAAIPALLVVAGLHHHLIRRGTRTRVSLLVESGEPREVHHFACLVGYGADAVNP
                     YLAFETIRDLIEHRLLTGIDYDTAAGKYLKATTKGVVKVISKMGISTVQSYHGAQIFE
                     ALGLGRSVIDRYFTSTPTRIEGADMNVLADEAQQRHDAAFPERPANGHTLEAGGRYQY
                     REGGEFHLFNPSTIHRLQHAVRSGDYEAYKLYAAEIHDRETHLATLRSLLELSTDCAP
                     IPLDEVEPVEAIMTRFKTGAMSYGSISQEAHETLAIAMNRVGGKSNTGEGGEDSERYA
                     SDPDGRFNRNSAIKQVASGRFGVTIEYLNQAREIQIKMAQGAKPGEGGQLPGHKVYPW
                     IARTRHSTPGVGLISPPPHHDIYSIEDLAQLIYDLKNANPQARINVKLVSEVGVGTVA
                     AGVAKGHADVILISGHDGGTGASPASSIKHAGLPWELGLAETHQTLVLNNLRGRVRLE
                     TDGQLKTGRDVVIAAMLGAEEFGFATGPLVAMGCIMMRVCHLDTCPVGIATQNPELRE
                     RFDGRPE"
BASE COUNT          606 a         1093 c         1139 g          623 t
ORIGIN      
        1 tgctccggcc ggccgtcaaa gcgctcgcgc agttcggggt tttgcgtggc aatgccgacc
       61 ggacaggtat ccagatggca aacgcgcatc atgatgcagc ccatcgccac cagcgggccg
      121 gtggcaaagc cgaactcctc ggctcccagc atggcggcga tgaccacgtc gcggccggtt
      181 ttgagctggc cgtcggtctc cagccgcacc cggccgcgca agttgttgag cacaagcgtc
      241 tggtgggttt cggccaggcc cagctcccac ggcaggccgg catgtttgat gctcgacgcc
      301 ggtgacgcgc ctgtgccgcc gtcatggcca ctgatgagga tgacatcggc atggcccttg
      361 gcaacaccgg cggcgactgt gcccacgcca acctctgaga cgagcttgac gttgatgcgc
      421 gcctgcggat tggcgttctt caggtcgtag atgagctggg ccagatcttc gatggaatag
      481 atgtcgtggt gcggcggcgg cgagatcaag ccgacgcccg gcgtggaatg gcgcgtccgg
      541 gcgatccagg ggtagacctt gtggccgggc aactgcccgc cctcgcccgg cttggcgccc
      601 tgggccatct tgatctgtat ctcccgcgcc tgattcaaat actcaatggt gacgccgaag
      661 cggccgctgg cgacctgctt gatggcgctg ttgcggttga agcggccgtc gggatcggac
      721 gcgtaacgct cggagtcctc accaccctcg ccggtgttgc tcttgccgcc cacccggttc
      781 atagcgatgg ccagcgtctc gtgcgcctcc tggctgatcg agccatagga catggcgccg
      841 gtcttgaagc gcgtcatgat cgcctccacc ggctcgacct catccagcgg gattggggca
      901 cagtcggtgc ttagttccag cagggagcgc agcgtggcca gatgcgtctc gcgatcgtga
      961 atctcggccg catagagttt ataggcctcg taatcgcccg accgcacagc gtgctgcaaa
     1021 cgatgaatcg ttgacgggtt gaacagatga aactcgccgc cctcgcgata ctggtagcgg
     1081 ccgccggctt ccagcgtgtg accgttggcc gggcgctccg ggaaggcggc atcatggcgc
     1141 tgctgtgcct cgtcggccag cacgttcatg tccgcgccct cgatccgcgt cggcgtgctg
     1201 gtgaagtagc ggtcgatgac actccggccc agaccaagcg cctcgaagat ctgcgccccg
     1261 tgatagctct gcacggtgga gatgcccatc ttggagatga ccttgaccac gcccttggtc
     1321 gtggccttga gatatttccc ggccgcggtg tcgtagtcga tgccggtcag caagcggtgc
     1381 tcgatcagat cacggatagt ttcgaacgcc agataaggat tcaccgcgtc ggccccatag
     1441 ccgaccaaac aggcgaagtg gtgaacttcg cgcggttcgc ccgactccac gagcaggctg
     1501 acgcgagtgc gcgttccccg acgaatgaga tgatggtgga gtccggcgac gaccaacagc
     1561 gcggggatgg ccgcttcatc gcgactgatc ccgcggtcgc tcaggatgat gagattggcg
     1621 ccaccggcaa gcccacgatc ggcggtggcg aacaacttgt ccagcgcggc cgccagtccc
     1681 gttcccccac tacccgacgg gaacagcatc ggcaaggtga cggcgctgaa cccggcgcga
     1741 tacggtttgt ggccgccgat gccgcgcaat cttgccagtt cggcgttggt cagcacgggc
     1801 gattgcagac gaatccggcg gcaatcttcc ggccgcgggt tcacaaggtc accttcggaa
     1861 ccgagcagca tcgcgctgct ggtcactaac tcctcgcgga tggcgtcgat gggcgggtta
     1921 gtcacctggg cgaacagctg gcggaagtaa tgatagagca gttgcggccg tcgcgagagt
     1981 gcggcaatag gcgtgtcgtc gcccatggac gccagcggtt caaccccgtc gcgggccatg
     2041 ggggccagga tcatgcgcaa gtcctcgaac gtgtagccga aggcgtgctg tcgcaacacg
     2101 atggtgtcat ggcccggagc gcgctcgcgg ccgccctccg gcagatcctc gagcgttagc
     2161 atgtggcggg ccagccactc gccgtaaggg tgttccgcag ccacgcgctg cttcaactca
     2221 tcgtcatcga cgatactctc ctcgctcgtg tcgaccagca gcatacggcc gggctggaga
     2281 cgacccttat agcggatcag ctccggcggc acgtcgagca cgccgacctc ggaggccata
     2341 atcacccgat cgtcggccgt cacgtagtag cgcgacggcc gcagcccgtt gcggtcgagc
     2401 accgcgccga ccaccacgcc gtcgctgaag acgatcgacg ccgggccatc ccacggctcc
     2461 atcagggcgc tgtggtaggc gtagaaatca cgtttcccgg cgctcatctc ggggttcttc
     2521 tcccacggtt cggggatcat catcatgacg gcatgaggca gcgaacgccc ggccagcgac
     2581 agaaactcca atgcgttgtc gaacatggcc gaatcggtgc cgtcttcgtc gatgatcggc
     2641 agcaccttgg gcagatcgtc cccgaacaga tcggagtgaa acagcgcctg ccgcgcgcgc
     2701 atggcgttga cgttgccttt gatcgtgttg atctctccgt tgtgggccag aaagcggctg
     2761 ggatgggcgc gcttccaggc cgggaaggtg ttggtggaga agcgggagtg aaccagggcg
     2821 atggcggaat cgatcagcgg atgggacaga tcggggaagt agccgcgcag ttgctcagcc
     2881 agcagcatcc ccttgtagac gatggtgcgg ctcgaaagag aagcgatata gaaggccgca
     2941 tccccttcgc tcgcgtcgcg gccgatctgc ttctcggcca gccggcgaat gacgaacaat
     3001 ttgcgctcga aggccaaggg gtcgtcggac aggccctgac cgcggccgat gaacacctgt
     3061 cgcataaccg gctcgctgcg cacggccgtt gcccccagat cggcgttgct cgtcggcaca
     3121 tcgcgccagc cgagaagcgt ctgcccttcg gcggcgacaa tggccgccag cgtcgcctcg
     3181 cattcggctc gccgggcagc ttcgcgcggc aggaacacca tgccgacgcc atacgctccc
     3241 ggaggaggca ggtggatatt cagttgttgc gttaattgtg aaaaaaggtt gtggggaatt
     3301 tgaatgagga tgcccgcgcc gtcgcccgtg ttcgggtccg cgccgcgcgc gccgcggtgg
     3361 ctcatgtttt caagcatggt cagcccatcg gcgataatgg cgtgcgatcg tcggcccttg
     3421 atgtcggcga gaaagcccac gccacaggct tcatgctcga a
//
LOCUS       DAOMQG010000358         3453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11172_length_3453_cov_7.781342, whole genome shotgun sequence.
ACCESSION   DAOMQG010000358 DAOMQG010000000
VERSION     DAOMQG010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11172_length_3453_cov_7.781342"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1380)
                     /locus_tag="PLC06_14460"
     CDS             complement(337..1380)
                     /locus_tag="PLC06_14460"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRO91697.1"
                     /translation="MKRTSHLFTLAVAIFAMLLLVACTVQPEAEEAEQVPVEINRDTL
                     KIFGTLPEEATPADYEMSDELIDLGRILYYEPRISATGNMSCNTCHGLNNYGIDGLRF
                     SFGHTGNPVGRNSPSVYNAALHVSQFWDGRAATVEEQAKGPILASGEMGMPSGDKVVE
                     VLKSISGYLPYFEAAFPGQDDPITYDNVGTAIGAFERRLMTPGRFDEFLAGDETALTE
                     REKAGLALFMGTGCIGCHNGPALGGNSYAALGSLEPYTTNDLGRYVVTGNEAEKFVFK
                     VPSLRNVAVTGPYLHDGSIPTLESMVQLMVRHQLGRELTSEEVDTMVVFLRSLTGELP
                     SDYTAEPQLPLND"
     gene            1815..>3453
                     /locus_tag="PLC06_14465"
     CDS             1815..>3453
                     /locus_tag="PLC06_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91698.1"
                     /translation="MKKIAPVLSLALLLIAAFVLPAAVRPSLVAAQTPSTLIDRAGGA
                     YNGIEVTLAADAAPAPGQVVTLTLTARPLRTAPNVYIEWALPDGGELLGGPAEETLGP
                     VGAGETLTRQARFADGVHQVSARAFYFPNRATSLAALGVLFFDVRAGNPAVSTLDPRT
                     PVYTSPSARQTVDKSHLATSGPVDRATTGCFSVHGILTRENRMPITSVIPEPGLPAGT
                     PPLYAGSYQDQLGSAVPVHHILVEMREEDTISDDSYGHTVTDANGKFTFNFCDDDGFL
                     NDELELYFRVCAEVRDGNSLIAAIKNLDEQELYCWDSNVIDSEGGDVDFDLTVYKLNQ
                     AEASVFNIADSLYWAWRFWNNNTANSPVMDRAVTAFWQGGQGRTGSFYNRGRTSLVIA
                     DDPSSADEWDDSVIIHEWGHFADNQFSCYQNPGGAHSLPGVNAGMNATRLAWGEGYPD
                     YYQSVARTILPGSAAINFYVDPSGPTVDLENMRAVTAADTDEGAVAAMLWDFHDTTND
                     GQDTVSHGHAAIQRIYTTGDFRNNAKCDARRFIEVWRN"
BASE COUNT          694 a         1078 c          994 g          687 t
ORIGIN      
        1 atagggatta ggcgccgaca tgacggcgat catagcacaa cccatgccat gctataaaac
       61 caggtttccc gtcgggctgg agccgttgaa cgagcgactg aatacgactt gtccacgacg
      121 cggacttctt cggcgttaga gcctactcat ggcatagcct gggccatgta tgaactggat
      181 gtgtggggaa gatcggcgag tgacgaagtg cgaatcgaaa attagacgtg gcgatgtcgg
      241 gagccatcgg aatggatcgc ccgccacagc aaaacgcccc cggccctgaa atgatccggg
      301 ggcgctctgt ctaacaagcc aatgactcgc tacaaattaa tcgttgagag ggagctgcgg
      361 ctcggccgtg tagtccgacg gcaattcgcc ggtcaacgag cgcaggaaga cgaccatggt
      421 atcaacttcc tcgctggtaa gctccctgcc gagctgatgg cgaaccatca gttgcaccat
      481 cgattccagt gtcgggatgc tgccgtcgtg gagatacggg ccggtgacgg cgacattacg
      541 cagactcggc accttgaaga caaacttttc agcttcattg ccggtcacta cgtaccgtcc
      601 cagatcgttc gtcgtataag gttcgagcga gccaagggcg gcatagctgt tgccgccaag
      661 cgccgggccg ttgtggcagc cgatgcagcc agtccccatg aacaatgcca acccggcctt
      721 ctcccgttcc gtcagggctg tctcatctcc ggccaggaac tcgtcgaagc gtcccggtgt
      781 catgaggcgg cgttcgaacg caccgatggc tgtgccgaca ttatcgtagg tgatggggtc
      841 gtcctgcccc ggaaaggcag cttcgaaata gggcagatag ccggagattg acttgagcac
      901 ttcgacaacc ttgtcgccgc tgggcatccc catttctccg ctggccagga tggggccttt
      961 ggcctgttcc tcaacggttg cggcgcgtcc atcccagaac tgggaaacgt gcagcgccgc
     1021 attgtagacg ctgggcgaat tgcggccgac gggattgccg gtatggccga aggaaaagcg
     1081 caacccgtca ataccatagt tgttaagccc atgacaggta ttgcacgaca tgttgccggt
     1141 agccgatatg cgcggctcgt aatacagtat gcggcccagg tcgataagtt cgtcgctcat
     1201 ctcgtagtcg gccggcgtgg cttcctcagg caaggtgccg aaaatcttca gtgtgtcgcg
     1261 gttaatctcg accgggactt gttcggcttc ctcggcttcc ggttgcactg tgcaagcgac
     1321 aagcaagagc attgcgaata tagccacagc caatgtgaat agatgtgagg ttcgtttcat
     1381 cattacttct cctgattcta aacaacaaca ggctcggatt gcgggattag agggccgcca
     1441 aatttgcctg ccccgggccg caaacaatac caacgatgag ttcgcgtgct catcgccgca
     1501 tattaatcag attaaacccg ccggagtgcc ataatcataa tgaccaaaat cacctgtttt
     1561 tgagatgaat gtaatgtttc ggctgttcca tacaaccggc aaggcggtcg gctcattggg
     1621 tcatcgcctc ctttgcccat gtcatggagt tgtccgtgcc acggagttgt ccgtgccacg
     1681 gggttgtccg gcttttgatt gcaaataaaa gtccccctat tgcattccac ctcaccctct
     1741 gctagtatgt tcccggaacg gggcaagcca atcgaataca aaccgaacag ggccgttata
     1801 gcccaaggtg acccatgaag aagatcgctc ctgttctctc ccttgccctc ctactcatcg
     1861 cggcatttgt cttgcccgct gctgtgcgcc cctcattagt cgcggcgcaa accccgtcca
     1921 cccttatcga ccgcgccggc ggcgcctata atggcatcga ggtcacgctg gcggccgacg
     1981 ccgcccccgc gcccggccag gtcgtcaccc tgaccctgac tgctcgcccg ctgcgcaccg
     2041 cgcccaatgt ctacatcgag tgggcgctgc ccgacggcgg cgaactgctc ggcggcccgg
     2101 ccgaggaaac gctcggcccc gtcggtgcgg gcgagacgct cacccggcag gcgcgcttcg
     2161 ccgacggcgt ccatcaggtc agcgccagag cgttctactt tcccaaccgc gccacctcgc
     2221 tggccgcgct gggcgtgctg ttcttcgacg tgcgcgcggg caacccggcc gtttccacgc
     2281 ttgacccgcg cacgccggtc tacacgtcgc cgagcgccag gcagacggtg gacaaatccc
     2341 atctggcgac gagcggccct gtcgatcggg ccacgaccgg ctgcttcagc gtccacggca
     2401 tcctgacccg cgagaatcgc atgccgatca cctctgtcat ccccgaaccc ggcttgccgg
     2461 cgggtacgcc gccgctctat gccggcagct atcaggatca gttgggatcg gccgtgcccg
     2521 tccatcatat cctggtggag atgcgcgagg aagacaccat ctccgacgac tcctacggcc
     2581 acaccgtcac cgacgccaac ggcaagttca ccttcaactt ctgcgacgac gacggcttcc
     2641 tcaatgatga actggaactg tacttccgcg tttgtgccga agtgcgcgac ggcaatagcc
     2701 tgatcgctgc gataaaaaat ctcgatgagc aggagttgta ctgctgggat tccaacgtca
     2761 tcgattccga gggcggcgat gtcgatttcg atctgaccgt ctataagctc aatcaggccg
     2821 aggcatccgt tttcaatatc gccgattcgc tctattgggc gtggcgattc tggaataaca
     2881 acacggccaa ctcaccggtc atggatcgcg ccgtcaccgc cttctggcag ggcggtcagg
     2941 ggcgaaccgg ttcgttctat aacagggggc gcacgtctct ggtcatcgcc gacgacccgt
     3001 ccagcgccga cgaatgggac gactcggtca tcatccacga atggggccac ttcgccgata
     3061 accagttcag ttgctatcaa aaccccggcg gcgcgcactc gctgccgggt gttaatgcgg
     3121 gcatgaacgc cacgcggctg gcctggggcg agggctatcc cgactactac cagtccgtgg
     3181 cccggacgat cttgcccggc tcggcggcga tcaacttcta cgttgaccct agcggcccga
     3241 cggtcgatct ggagaatatg cgcgccgtga cggcggctga taccgacgag ggggccgtcg
     3301 ccgcaatgct gtgggatttc cacgacacga ccaacgacgg ccaggacacg gtcagccacg
     3361 gccatgccgc catccagcgt atctacacca ccggagactt caggaacaac gccaagtgcg
     3421 acgcgcgccg ctttatcgaa gtgtggcgca att
//
LOCUS       DAOMQG010000359         3445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11205_length_3445_cov_10.810324, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000359 DAOMQG010000000
VERSION     DAOMQG010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_3445_cov_10.810324"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLC06_14470"
     CDS             complement(<1..925)
                     /locus_tag="PLC06_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRO91699.1"
                     /translation="MRIAIFAGGSGRRLWPISRQSSPKQFEPIVGELSTLQLAMERVA
                     PQYGYANVFVSTNERYGEIVCYQLPELPPANIIFEPARRDLGPAVGLAMAHLAADSVD
                     EPVAILWSDNYIENNVAFADLLKVAERLIVEQKAQIIFIGETPRFANENLGWIGLGKE
                     KGIIDDTPYFGFESLTYRPSLDQCRMIFEQGTHVWNTGFFVTTPAYIQQLYKRFSPDM
                     WSQLQEIEASIGAEAYRDVLARIYPEMRAISFDDAILTHLDPEDALVLHSEMGWSDPG
                     TLYALKEALNPDRDANVIKGLVIDTESRDCLI"
     gene            complement(949..2079)
                     /gene="dprA"
                     /locus_tag="PLC06_14475"
     CDS             complement(949..2079)
                     /gene="dprA"
                     /locus_tag="PLC06_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRO91700.1"
                     /translation="MTELKYWLGFNYVSGIGPAKIQALLGYFNTLEKAWNASDEQLRD
                     IGFDIRAIQSLNEVRQNFDLDQYVRQVETSGVGVVTWGSPEYPELLREIPAAPPLIFL
                     RGQLEPIDRWAMAVVGTRRLTAYGRQVTRDLVAGLVVNGITVVSGLARGIDAVAHRTA
                     LEDGGRTIAVMASGIDKVYPPEHRDLAREIVAGRGAIISDYPFGAEPESSHFPARNRL
                     ISGLSLGVIVIEAGERSGALITARFALEQNREVFAVPGNIHSPVSVGTNRLIQQGGKL
                     VMRVEDILEELNLRMAAEQAVAQVVLPETAEEAALISQLSSQPIHVDELGRLTGMPMS
                     LISSTLTMMELKGMVQQVGGMNYIRLREEGPIYNAGTASGQE"
     gene            complement(2142..2732)
                     /gene="rimM"
                     /locus_tag="PLC06_14480"
     CDS             complement(2142..2732)
                     /gene="rimM"
                     /locus_tag="PLC06_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRO91701.1"
                     /translation="MSKKDQISKPESTQSSNRGSVDESAEPRFLAVGRIAKPHGVLGE
                     VRVELLTDHPERFEWLDAVYVGEKDPRRVPIVSVRYHQDVVLLKLDGYPTRTEAELLR
                     GELLQVPESEAVPLEEGEYFLYQLIGVEVFTENGHRVGQLTEILETGANNVFVVDDRN
                     NQYLIPDIPDVIREVDIENRRLVIYPMPGLVAGLDE"
     gene            complement(2743..2988)
                     /locus_tag="PLC06_14485"
     CDS             complement(2743..2988)
                     /locus_tag="PLC06_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRO91702.1"
                     /translation="MKDLVAYLVESLVDNPEEVTVTEVRDRATTVVELAVAAPDMGRI
                     IGKGGRVIDSIRALMQVRAAKEGRRVELELLEADEMR"
     gene            complement(3222..3445)
                     /gene="rpsP"
                     /locus_tag="PLC06_14490"
                     /pseudo
     CDS             complement(3222..3445)
                     /gene="rpsP"
                     /locus_tag="PLC06_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S16"
BASE COUNT          796 a         1029 c          846 g          774 t
ORIGIN      
        1 agatcaggca atccctcgat tctgtgtcaa ttaccaaacc cttaatcaca tttgcgtcac
       61 gatcaggatt aagcgcctct ttcaaagcgt agagagtgcc cggatcgctc catcccatct
      121 cgctatgaag aaccaaggca tcttccgggt caaggtgggt caggatcgca tcatcaaagc
      181 tgatggctcg catttcagga tatatccgag ccaaaacatc tctataggct tcggcaccaa
      241 tcgacgcctc gatctcctgc aattgtgacc acatatccgg cgaaaaccgc ttatagagct
      301 gctggatata cgccggggtg gttacgaaaa agcctgtatt ccagacatgg gtgccttgct
      361 cgaaaatcat ccggcattgg tcaagcgaag ggcgataagt gagcgattca aatccaaagt
      421 agggggtatc gtcaatgata cccttctctt tgcccaaacc aatccagccc aggttttcat
      481 tggcgaatcg tggtgtttca ccaataaaga taatctgtgc cttttgctca acgatgagcc
      541 gctcggcaac cttgagcaaa tcggcaaagg cgacgttgtt ctcaatatag ttatcggacc
      601 agagtatggc aaccggttca tcgacactat cggcagctaa atgagccatc gccaggccaa
      661 cggccggccc taaatcgcgg cgcgccggct caaagatgat attggcgggg ggaagttcgg
      721 gaagctgata acaaactatt tcgccataac gctcattggt cgatacaaag acattggcat
      781 agccatattg cggggcgaca cgctccatag caagctgaag ggtcgatagt tccccgacaa
      841 taggctcaaa ttgcttgggt gagctttgcc tgctgatcgg ccaaagcctt ctgccggaac
      901 ccccggcaaa tattgcgata cgcatgatcg taagtttcct tcccggtttt attcctgacc
      961 gcttgccgtg ccggcattat agatcggccc ttcttcccgc agacgaatgt aattcatgcc
     1021 gcctacttgc tgaaccattc ctttgagttc catcatcgtc agggtgctgc tgatcaggct
     1081 catcggcatg cccgtcaggc gacccagttc atcaacgtga attggttggg aagacaattg
     1141 tgatatcagg gcggcttcct cagctgtctc cggcaataca acctgcgcca cagcttgttc
     1201 cgcagccatt ctcagattca attcttccag aatatcttca acccgcatca ctaactttcc
     1261 accctgctga atcaaacgat tcgtgcccac gctaacaggg ctatggatat ttcccgggac
     1321 ggcgaacact tcgcggttct gctccagggc gaagcgggca gtaatgagcg cgccacttct
     1381 ctcgccggcc tcgatgacga tcacgcctaa cgacaagcca ctgatcagtc gattgcgcgc
     1441 cgggaaatgg ctactttccg gctcggcccc aaagggataa tccgatatta tcgccccacg
     1501 accggccaca atttcacgag cgagatcacg atgctcaggc gggtagacct tgtcgatgcc
     1561 cgaagccatg acggctatgg ttcgaccccc atcttccaac gctgtgcgat gggccacagc
     1621 atcgataccg cgcgccagtc cgctgacgac cgtaattcca ttaacaacca gccccgcaac
     1681 cagatctcgc gtcacttggc ggccataggc cgtcagacgg cgcgtgccca ccacggccat
     1741 cgcccatcga tcaatgggct ccagttgccc tcttagaaag atgaggggcg gcgcagcagg
     1801 gatctctctg agcagttcgg gatattcagg agagccccag gtcaccacac caacgccgct
     1861 ggtttcaacc tgtcgcacgt attgatcgag gtcaaagttt tgccgcactt cattcaggct
     1921 ctgaatggcg cggatgtcaa atccaatatc gcgaagttgt tcgtctgagg cgttccaggc
     1981 cttttccaac gtattgaaat accccaataa agcctgtatc ttggccggcc ctatcccgct
     2041 tacataattg aatcccagcc aatatttcag ctcggtcatt ggggttctgc ctaatcgttc
     2101 acaagctatc agggccagag gatgccgaga tatgaaaccg gctattcatc cagaccggcg
     2161 accaggccgg gcattggata gataacaagg cgtctattct cgatatcgac ctccctgatc
     2221 acatcgggaa tatccggaat aagatattgg ttgttcctgt catcgacaac gaaaacgtta
     2281 tttgcgcccg tctcaaggat ctcggtcagt tgcccaacgc gatgaccatt ctccgtaaaa
     2341 acctcaacgc caataagttg gtagagaaaa tactcaccct cctcaagggg gacggcctcg
     2401 gattcgggaa cctgcagcaa ctcgccgcgc agcaattccg cctccgtgcg cgtagggtag
     2461 ccatccagct tgagcaagac tacatcctga tggtatcgta cgctcacgat gggcacgcgc
     2521 cgagggtcct tctcgcccac gtaaacggcg tccagccatt caaaccgctc aggatgatcg
     2581 gttagaagct ccactcgaac ctcacccaaa acgccgtggg gttttgcaat tcgtccaacc
     2641 gctaaaaaac gaggctcggc agattcatct acggagcctc tattcgacga ttgagttgat
     2701 tcaggtttgg atatctgatc tttcttgctc aaccgatccc cctcatcgca tctcgtccgc
     2761 ctcaagcaac tcaagttcca cgcgacgccc ttctttggcg gcacgaactt gcatgagcgc
     2821 gcggattgag tcgatgacgc ggccgccttt cccaatgatt cgccccatat cgggcgcggc
     2881 cacggccagt tctacaaccg tggtggctcg atcacgaact tcggtgaccg taacttcctc
     2941 gggattatca acaagcgact caaccagata ggcgacaaga tccttcatga gatttattct
     3001 tcctcgtcgg catccagaac cgcgtcgata gcctcttcga tcttctcgac cgcagtttcg
     3061 gcaacatctt caactttctc ggcaactgcg gaagctgctt cggcgacggc ggcaacaact
     3121 ccaccggcgg caacgggcga taccgccgcc tcgggggcta cgccggtgaa ctcactcacg
     3181 agcacatcca tggactcgcc ggcatgataa cgagacaagc gcccgatggt gccctgcttc
     3241 tccagcagaa tgcgcactgc gtccgacggc tgggcgccga cgcttagcca gtacagcgcc
     3301 cgtgcttcat cgatcttgta atcagccggg gttgcgagcg ggttataata accgatgcgc
     3361 tcgacaacac gaccatcgcg cttggcttcc ttctccgcca ccacaacgcg atagctgggt
     3421 tggcgtttct ttcctgttcg gctta
//
LOCUS       DAOMQG010000360         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11263_length_3431_cov_8.689573, whole genome shotgun sequence.
ACCESSION   DAOMQG010000360 DAOMQG010000000
VERSION     DAOMQG010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11263_length_3431_cov_8.689573"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1561)
                     /locus_tag="PLC06_14495"
     CDS             complement(101..1561)
                     /locus_tag="PLC06_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HRO91703.1"
                     /translation="MKKVASPADPPADFLARMHDLLGPDEYAAFLSAFDQPPRVGLRV
                     NTLKISVPDFISIAPFSLAPVGNWEPAGFFVTDQSRPGRHPYHDAGLYYLQEPSAMVA
                     AALLAAAPGELVLDLAAAPGGKATHLASLMAETNSDKSLSPLRRALDSPGLLVANDVH
                     AGRARLLADNLARWGAGNVLVIQDEPERVAAAFGPVFDRMLIDAPCSGEGMIRRRESL
                     EWSEAIVAACARRQRDIMTVAPDLVRPGGRLLYATCTFAPEEDEQIVAGFLADNPEFS
                     LVEPRRFEGFERGRPEWAGNAGESAAQLARAVRLWPHRFPGEGHFLALMAREATAHRV
                     TRPQGAFRPTPPMREEMRLWHEFAEATLTVDIPTERLNAHNGRLYLLPQTTLPTGNLH
                     IIRYGLLLGEMRRGRFHPAHELALAMAAGDATHVLVFAAEDTSLAAYMAGVDLPSAGP
                     DGWTLVAIEGHGLGWGKRVRGKVKNHYPHYLRRKVI"
     gene            1574..2668
                     /locus_tag="PLC06_14500"
     CDS             1574..2668
                     /locus_tag="PLC06_14500"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015164297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRO91704.1"
                     /translation="MTSDRTLSLPHGDLELPAFLPDGTQGVVRSVSAQDLEACRVRAV
                     QMNVYHLMQRPGTSTIQALGGLHRMASWSRPIFTDSGGFQVYSLIRQNPKAGSITDRG
                     ATFRPGGDRKYILTPEKSIQLQFGYGSDMLICFDDCTGPDDPPAGQADSVRRTIRWAA
                     ECKKTYLRLVEQKRLGEAERPRLMAVIQGGLSPDLRRECAEALLDIGFDAYGYGGWPL
                     DASNNLLTEMVGLVRELVPREFPVHALGIGHPANVVACAKMGYELFDSTMPTRDARHG
                     RLLVFTRDPAAAPLSSGNDWFAYLYPGDDKHVKAAVPVSPYCDCPTCTHYSLAYLHHL
                     FKINDTLYFRLATTHNLRFMTMLMERLREA"
     gene            2826..3020
                     /locus_tag="PLC06_14505"
     CDS             2826..3020
                     /locus_tag="PLC06_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRO91705.1"
                     /translation="MKETGNTRLLRRQNGMIGGVCGGLAEFTGISAFWFRLLFLILLL
                     PGGVPGLLPYLLLWIVIPKK"
     gene            complement(3108..>3431)
                     /locus_tag="PLC06_14510"
     CDS             complement(3108..>3431)
                     /locus_tag="PLC06_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019549999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRO91706.1"
                     /translation="TTSPDLSLYEWFVAPHRQDGLDFHFYMIDHSWAGTTDDFRFDLL
                     EPRLQLYGDTAVVSYTFMLSVAKADGIRHTLHNESRVLVKRGGAWQVVHVHKSPAWKA
                     PREQP"
BASE COUNT          613 a         1082 c         1097 g          639 t
ORIGIN      
        1 gacgactaac gcccggctta tcgtcgacac gcgtaatgcg gttggttctt cgggggtggc
       61 ccgcgtcgtg cgtctgtagg gagcctcttg ggactttgtg ttaaataact tttcgccgca
      121 aataatgggg gtagtggttc ttgaccttgc ccctcacgcg ctttccccag cccaacccgt
      181 gcccttcgat cgccaccagc gtccagccat ccggcccggc tgaaggcagg tcgaccccgg
      241 ccatataggc tgccaatgat gtatcttcgg ccgcgaagac aagcacgtgg gtagcgtccc
      301 cggccgccat cgccagcgcc agttcgtggg ccggatgaaa tcggccgcgc cgcatctcgc
      361 ccagcaacaa gccgtagcgg atgatatgga gatttcccgt gggcaaggtg gtttgcggca
      421 gcaggtagag acggccgttg tgggcgttca gtcgctcggt gggaatatcg accgtcaggg
      481 ttgcctcggc gaactcgtgc catagacgca tttcttcgcg cattgggggc gttggcctga
      541 atgcgccttg tggtcttgtg accctatgag ccgttgcctc tcttgccatg agcgccagaa
      601 aatgcccctc gccggggaag cgatgcggcc acagacggac ggcgcgggca agttgtgcgg
      661 ccgattcacc cgcattgccc gcccactccg gccggccgcg ctcgaacccc tcgaagcggc
      721 gcggctccac cagcgaaaac tccgggttat cggccaggaa ccccgccacg atctgctcat
      781 cttcttccgg tgcaaacgtg caggtggcgt agaggagacg gccgccgggt cgcacgagat
      841 cgggagcgac ggtcatgatg tcgcgctgtc gccgggcgca ggcggcgacg atcgcctcgc
      901 tccactccag gctctcgcgg cggcggatca tcccctcgcc ggagcagggc gcgtcgatga
      961 gcatgcggtc gaagacgggg ccgaacgcgg cggccacgcg ctccggttca tcctggatta
     1021 ccagcacgtt gcccgctccc cagcgcgcca gattgtcggc cagcaggcgg gcgcgcccgg
     1081 catggacatc gttggccacg agcaaacccg gtgaatccaa ggcccgccgc agcggggaga
     1141 gcgatttgtc ggaatttgtc tcggccatca gacttgccag atgggtcgcc ttgccgcccg
     1201 gcgcggccgc cagatcgagc accagttcgc ccggcgcggc cgcgagcagc gcggcggcga
     1261 ccatggccga cggctcttgc aggtaataca atcccgcgtc gtgataggga tggcggccgg
     1321 ggcggctctg atcggtcacg aagaaacccg ccggctccca attgcccacc ggcgcaagcg
     1381 aaaacggtgc gatgctgata aaatccggca ccgatatctt gagcgtgttc acccgcagcc
     1441 cgacgcgcgg cggctgatcg aaggcggaaa ggaaagccgc atactcgtcc ggccccaaca
     1501 ggtcatgcat gcgggccaga aagtcggccg gcggatcggc gggcgaagca acctttttca
     1561 tctcttgaga actatgacga gcgatcgaac cctgtccttg ccccacggcg atctggagtt
     1621 acccgcgttt ttgcccgacg gcacgcaggg tgtggtgcgg tctgtgtcgg cccaggacct
     1681 cgaagcgtgc cgcgtgcgag cggtgcagat gaacgtctat cacctcatgc agcggccggg
     1741 cacatcgacg attcaggcgc tgggcgggct gcaccggatg gcgagttggt cgcggccgat
     1801 cttcaccgac tccggcggct tccaagtata ctcgctcatc cggcagaacc ccaaggccgg
     1861 ctccatcacc gatcgcggtg cgaccttccg ccccggcggc gatcgcaaat acatcctgac
     1921 gccggagaag agcatccagc tccagttcgg ctacggcagc gacatgctca tctgctttga
     1981 cgactgcacc ggccccgacg acccgcccgc gggccaggcc gattccgtgc gccgaacgat
     2041 tcgctgggcg gccgaatgca agaagacgta cctgcggctg gtggagcaga agcggctggg
     2101 cgaggcggag cggccgcggc tgatggccgt cattcagggc gggttgtcgc ccgatttacg
     2161 gcgggaatgc gccgaggcgc tcctcgacat cggcttcgac gcctatggct acggtggctg
     2221 gccgctggac gcgagcaaca acctgttgac ggagatggtc ggtctggtgc gtgaattggt
     2281 gccacgagag ttccccgtcc acgcgctggg aatcggccat ccggccaacg tggtggcttg
     2341 cgcgaagatg ggttacgaac tgttcgacag cacgatgccc acccgcgacg cgcgccacgg
     2401 ccgtttgctg gtcttcacgc gcgatccggc cgccgcgccg ctctcatccg gcaacgactg
     2461 gtttgcctat ctctatccgg gcgacgacaa acacgtcaag gcggccgtgc cggtctcgcc
     2521 ctattgtgac tgcccgacct gcacccacta ctcgctggcc tacctgcatc atctcttcaa
     2581 gatcaacgat acgctctact tccggttggc caccacccac aacctgcgtt tcatgacgat
     2641 gctgatggag cgattgagag aagcgtagag accgagcctt cccggggtga tgttcaccaa
     2701 atctgtttcg tggcagtccg caacttctct agccgcggcg cgtattgatc cttgagaacg
     2761 gtacagaact atttaggatc gcgaacagcg cggggctctc cgcgacgcgg tcgggagtat
     2821 caacgatgaa agagactggt aacacgagat tgttgcgccg tcagaacggc atgatcggcg
     2881 gggtatgcgg cggcctggcg gagttcacgg gcattagtgc gttctggttc cgcctgctgt
     2941 tcctcatact cctgctgccg ggtggcgtgc ccggattgct gccctattta ctgttgtgga
     3001 ttgtgatccc caagaagtaa gaagcgattt tttcctgagc aggccggttg ggcggagagg
     3061 aaacttgccg ctaaccggcc tttttttatt tgtggggaat ggcctttcta gggttgctcc
     3121 cgcggcgcct tccaggccgg cgatttgtgc acgtggacga cttgccacgc gccaccgcgc
     3181 ttcaccagca cgcggctctc gttgtggagc gtatggcgaa tgccatcggc cttggccacg
     3241 ctgagcatga aagtatagct gacgacggcc gtatccccgt agagttgcag ccgaggctcc
     3301 agcagatcga aacgaaaatc gtcggtcgtg cccgcccacg aatggtcgat catgtagaaa
     3361 tgaaaatcca gcccgtcctg ccggtgggga gccacaaacc actcatacaa tgacaggtca
     3421 gggctggtgg t
//
LOCUS       DAOMQG010000361         3407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11359_length_3407_cov_10.840990, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000361 DAOMQG010000000
VERSION     DAOMQG010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11359_length_3407_cov_10.840990"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLC06_14515"
     CDS             complement(<1..1307)
                     /locus_tag="PLC06_14515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRO91707.1"
                     /translation="MLRRFGVNYAIFSMALDISLTLLALVLATKVVAFIPLHLTNLRG
                     QPTVAPYIYLLVPLLWGTSFLVLSAYDPKRIYKAMGEFQIVTLAAIASALVFAGLLFL
                     MERNFSRWVFVFFVLFDLTFLLGWRILARAIFRIGRMPAAERHVLIVGAGDVGRRVGQ
                     MIQEYRSMGLNLTGFLDETGNGHPESDEYMIHILGQVEDAREVIQQSNINDVVIALPQ
                     RDYAQINELVLALHDLPVQVRVVPDYFSLALYRASVEDFGGLPMINLRDPALNDVQRL
                     IKRMFDLALSSILIAIVLVPMAIITALIKLDSEGSVLFRQQRVGENGRLFSMYKFRSM
                     VVDADKMLDEVTELSAGGQVLFKREDDPRVTRVGRFLRHTSLDELPQLFNVLKGDMSL
                     VGPRPELPWLVGQYEPWQHKRLAVPQGMTGWWQINGRADKPMHL"
     gene            complement(1526..2293)
                     /locus_tag="PLC06_14520"
     CDS             complement(1526..2293)
                     /locus_tag="PLC06_14520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRO91708.1"
                     /translation="MRPPPINILGVQVHPLTVDQLHEELAQLIETDAHAEVLHVNVHG
                     MNLSADNPWLRDYLNQAEIVFCDGAGIILGARILGQHIPERITYADWMWQLAEFAAAR
                     GYSFYFLGAKPGIAQTAADRMIKRFPELKIAGVRDGYFNKDNGHPDNEAVLTDINAVR
                     PNILIVGMGMPMQERWLLDNWSRIDANIALTGGAVFDYISGDLKRAPAWMTNNGLEWL
                     GRLLIEPRRLWQRYIVGNPVFLARVIRERLHHHQAWY"
     gene            complement(2274..>3407)
                     /locus_tag="PLC06_14525"
     CDS             complement(2274..>3407)
                     /locus_tag="PLC06_14525"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRO91709.1"
                     /translation="GLFIEESMRYATFARNLGAFVSGFLVYLVITNRARSLRSIDKIL
                     NIILIAMILAGIAGLSGVLGIWRPSFQSPAGWLLPDSVVATSYGSVIAHRELGQYGWF
                     TGLGLYFRLSSFFLFGNHYSSAIVYTLPFLFMRMGQSRGVRKLAVGFAIPLLIINLIY
                     TTGRVAALSLLFGALFFALFHSVYRRTVRALLALGLSLAILAVLSLSIIEAGSPTAEA
                     GLIGQASSAVEAMVFARGPGSYESRFGLYGATLAGFLERPVFGWGTERDVEGLPLPAG
                     SHSEYLAALYRQGLLGLIALAGMVISAWLDTRPPRGAAARSREGTMLSYGRWFFVGMI
                     INNMMNDPAVDATAYVFMWAIIGLLVSTARLINKQEEDATTTD"
BASE COUNT          794 a          955 c         1017 g          641 t
ORIGIN      
        1 aggtgcatcg gtttgtcggc gcggccgttg atctgccacc agccggtcat gccttgcggc
       61 acggccaggc gcttgtgctg ccatggctca tattgcccca ccagccacgg caactccggt
      121 cgtggtccaa ccaggctcat gtctcccttg aggacgttga acagttgcgg cagctcgtcg
      181 agactggtat gccgcagaaa acggccgaca cgagtcaccc ggggatcatc ttcccgcttg
      241 aagagaacct ggccacccgc ggacaactca gtaacctcat cgagcatctt gtccgcgtcg
      301 acgaccatcg agcgaaactt gtacatcgaa aacagtcgcc cgttttcgcc aacgcgctgc
      361 tgccggaaca ggacagatcc ttcagaatcc agcttgatta gcgcggtgat gatcgccatc
      421 ggtaccagca cgatagcaat caggatgctc gatagcgcca gatcaaacat gcgcttgatc
      481 aagcgttgca cgtcgttgag cgcgggatcg cgcaggttga tcatgggcag gccgccgaaa
      541 tcttcaactg aggcgcgata cagggcaagg ctgaaatagt cgggcacaac tcgcacctga
      601 accggcaggt catgcagggc gaggacaagc tcgttgatct gggcatagtc ccgttggggc
      661 agggcgatga cgacatcgtt gatgttgctt tgttggatga cctcgcgcgc gtcctcgacc
      721 tggccgagga tgtggatcat gtattcgtct gactcgggat ggccattccc tgtctcgtcc
      781 aggaagccgg tcaggtttag gcccatcgag cggtattcct ggatcatctg tcccacgcgc
      841 cggccgacat ctcccgcgcc gacgatcagc acgtggcgtt cggcggcggg catgcggccg
      901 atgcgaaaga tcgcccgcgc caggatgcgc catccaagca ggaatgtcag gtcgaacagg
      961 acaaagaaga cgaacaccca gcgcgagaag ttgcgctcca tcaggaagag caagcccgcg
     1021 aaaacgaggg ctgacgcgat ggctgccagg gtgacgattt ggaattcacc catcgcctta
     1081 tagatccgct tgggatcata ggcggaaaga acgaggaagg aagtccccca cagcaaggga
     1141 accaggagat agatatatgg agcgacggtc ggctggccgc gcaggtttgt gagatgtaag
     1201 gggataaaag cgacgacttt tgtagccaac accaacgcca atagcgtcaa gctgatatca
     1261 agcgccatcg agaagatggc gtaatttacc ccaaaccgcc tcaacataaa atctcttcct
     1321 tctctgttgc cgaaacattg gccggagcgc tgtcaaagtt tcggccggct acacgcgaac
     1381 cttgcagcgc gtgcccagca aattgttatt gattggtaac gtgccgggcc ggttgtgaaa
     1441 ttcggtgcaa tccaaacaat tataacggcc tgcttcagac agacaaacag aattggaata
     1501 tatttactct tgtatgaatc ggtaattagt accaggcttg gtggtgatgc aggcgttcgc
     1561 gaatgacgcg cgccaaaaag acagggttgc cgacgatata gcgctgccac aatcgcctcg
     1621 gctcgatgag gaggcgacct agccactcca ggccgttgtt ggtcatccat gctggagcgc
     1681 gctttagatc gcccgatata tagtcgaaca cagcgccgcc ggtcagggcg atgttggcgt
     1741 caatgcggct ccagttgtcg agcagccatc gctcctgcat cggcatgccc atgccgacga
     1801 tcaggatatt ggggcgcacg gcgttgatat cggtcaaaac agcctcattg tcggggtggc
     1861 cattatcctt gttgaagtag ccgtcacgca cgccggcgat cttcagttcg gggaagcgct
     1921 ttatcatccg gtcagccgcc gtctgggcaa tgccgggttt tgcacccagg aagtagaagg
     1981 agtaaccacg cgcggcggca aactcggcga gctgccacat ccagtcggca taggtgatgc
     2041 gttcggggat gtgttgccca agaatgcgcg cgcccagtat gatccccgcg ccgtcgcaga
     2101 agacgatctc ggcctgattg aggtagtcgc gcagccaggg gttatcggcc gatagattca
     2161 taccatggac gttcacatgc agcacttcgg catgggcgtc ggtttcgatc agttgggcca
     2221 gttcttcgtg cagctgatcg acagttaatg gatgaacctg gacgccaaga atattaatcg
     2281 gtggtggtcg catcttcttc ttgcttgttg atcaatcgag cagtagacac caataggccg
     2341 atgatcgccc acatgaaaac gtaagccgtg gcgtcgactg cggggtcgtt catcatgtta
     2401 ttgatgatca tgcccacgaa gaaccagcga ccgtaagaca gcatcgtgcc ttctctgctt
     2461 cgtgcggccg cgccgcgtgg cggccgcgta tcgagccagg cagagatgac catgcccgcc
     2521 aacgcgatga ggcccagcag gccctgacgg tagagcgcgg ccagatattc gctgtgggag
     2581 cctgcgggca gcggtagacc ttccacatcg cgctcggtgc cccagccgaa aacaggtcgt
     2641 tcaagaaacc cggcaagcgt cgctccgtac aacccaaacc ggctctcata gctgcccggg
     2701 ccacgagcga agaccattgc ctctaccgca gagcttgcct gcccaatgag acccgcttca
     2761 gcggtgggcg aaccggcttc gataatcgac agggacagga cggccaggat agccagtgac
     2821 aggccgagcg ccagcaatgc ccgcacagtt cgccgataga cggaatggaa cagggcgaag
     2881 aacaacgcgc caaacagcaa gctcaaggcg gcgacgcggc cggtggtgta aatgagattg
     2941 atgatcagga gcgggatggc aaagcctacg gccaattttc gtaccccgcg cgattgaccc
     3001 attcgcatga aaaggaatgg cagggtgtag acgatagcgc tggagtagtg gttgccgaac
     3061 aggaagaagc tgctcaaccg aaaatacagg cccaggccgg tgaaccagcc gtattgcccc
     3121 agctcgcgat gggcgatgac cgaaccatag cttgtggcga cgaccgaatc cggcagcagc
     3181 caccctgcgg gcgattgaaa tgacggccgc cagataccca acacacctga caatccggct
     3241 atcccggcca agatcatggc gatgaggatg atgttcagga ttttgtcgat tgaacgaaga
     3301 gaacgggcgc gattggtgat gacgagataa accagaaacc cggatacaaa tgcaccgagg
     3361 tttcgcgcga atgtggcgta gcgcatcgac tcctcgatga agaggcc
//
LOCUS       DAOMQG010000362         3396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11412_length_3396_cov_10.272673, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000362 DAOMQG010000000
VERSION     DAOMQG010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11412_length_3396_cov_10.272673"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLC06_14530"
     CDS             complement(<1..821)
                     /locus_tag="PLC06_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRO91710.1"
                     /translation="MTFFRSGVNAWQRWSGQALEDRDQARDRRERALLLLVTVFIFVN
                     AIAYSLVTDDAVRWSHLFAPIVWLVVVGTAHLTLQTWRPRRDPFLLPVVALLSGWGLL
                     LQDNLAPNFLGRQTLWFALATMAMLVVAILPRTYAPLMRYRYSLLTGGLLLLAFTLIF
                     GVNPSGSGAALWLPAPFPFLGTVYFQPSELLKVLLVVFLASYFTELEPLYHYRKQAND
                     KVKGKSKTGNAFRQHLPFLGPLLLMWGFTLLLLVWQRDLGAAALFFIVFVAMLYLA"
     gene            complement(818..1690)
                     /gene="mtnP"
                     /locus_tag="PLC06_14535"
     CDS             complement(818..1690)
                     /gene="mtnP"
                     /locus_tag="PLC06_14535"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRO91711.1"
                     /translation="MSEVELAVIGGSGLYNMPGLSQVEMVEVDTPFGKPSDAITVGTL
                     HGRRVAFLPRHGRGHVLTPSEVPYRANIYALKTLGAKYVISVSACGSLREDFAPGDIV
                     IPDQLFDFTKGMRDYSFFGEGVVAHLAAANPFSVELNDALTNAVEIAGGKIHRGGRFI
                     TIEGPRFSTRGESEVYRQWGMSIIGMTTSPEAFLAAEAEMAYACMAHVTDYDCWHESE
                     EHVTVEMVIRTLKANTATAQAAISQLVMDFDKWAGNFAAHDTLKDALITDRAHLSTST
                     VETLRPIIGKYVGK"
     gene            1941..>3396
                     /locus_tag="PLC06_14540"
     CDS             1941..>3396
                     /locus_tag="PLC06_14540"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRO91712.1"
                     /translation="MKFLIALLTAATGILHLLVGFGMVGDDGMNWLLVINGVGYLVLL
                     VLFWRASGNGGAIRWILLAYALITFIGYFLLNRGANFSFSNTMGLIVKAIELLLILLL
                     FMYKGSQAAVAGSPAAGSTRMGSGIDATRSAGQAVGSSASKAAAGASAAAGAVTAASV
                     SVADRAGNAIAGAVDYVEDKAGDAVDAASDAAGDVVGAAEHAAGAVVDTTKDAVGYVG
                     DKAGEAVDAAADVAGDVAGAVDHAAEAAVDTAGDAVGYVGKKAGDAVDAAADVAGDVA
                     GAAEHAAEAAVDTAGDAVGYVGEKAGDAVDAAADVAGDVAGAAERAAEAAVDTTKDAV
                     GYVGEKAGEAVDAVSVDVEQAAEEPGEDEVRADLEEYLRSFGPSSEFRKPIEYIEGIG
                     AVYGQKLRSIEINTVMDLVLNGATRRGRKQIADQSGIAHSLILTWVNHVDLFRIKGVA
                     EEYADLLEQSGVDTVMELAQRNPANLYKRMKDVNE"
BASE COUNT          709 a          897 c         1062 g          728 t
ORIGIN      
        1 gccagataga gcatggccac aaagacgata aaaaatagcg ccgcggctcc cagatctcgc
       61 tgccagacca gtagcaacag agtgaaaccc cacattagca ataaggggcc gagaaatggc
      121 aggtgctgcc gaaacgcatt gcctgtcttg cttttacctt ttaccttgtc gttggcttgc
      181 tttcggtagt ggtagagcgg ttccagttcg gtgaaatagc tggccagaaa gacgaccaac
      241 agtaccttca gaagttcgga cggttgaaaa tagacagttc ccaggaatgg gaaaggagcc
      301 ggcagccaga gcgccgcgcc gctgccgctt ggattcacgc caaatatcag cgtgaaagcc
      361 agcaatagca atccgccggt cagcaaggag tagcgatagc gcatgagcgg cgcataggtg
      421 cggggcagaa tggcgacaac cagcatcgcc attgtggcca gggcaaacca taatgtctgg
      481 cggccgagga agttcggagc caaattgtcc tggagcaaca acccccagcc ggagagcaat
      541 gcgacaaccg gcagcaggaa agggtcgcga cgcggccgcc aggtttgaag cgtcaggtga
      601 gcggtgccca ccacgaccag ccacacgatc ggcgcaaaaa gatgcgacca acgcacggca
      661 tcgtcggtaa ccagactata ggcgatcgcg ttgacaaata tgaagacggt gactagcagc
      721 agcagcgccc gttcacgcct gtcccgcgcc tggtcgcgat cctccagtgc ctgaccggac
      781 catctctgcc aggcgttgac gcccgagcga aaaaatgtca tttacccacg tatttgccga
      841 tgattggccg gagtgtttcg actgttgaag tcgacagatg tgctcgatcg gtgatgagtg
      901 catccttcag cgtgtcgtgg gcggcgaaat tgccggccca tttgtcgaaa tccatcacca
      961 gctgactaat ggcggcctgg gccgtcgccg tgttcgcctt cagggtgcgg ataaccattt
     1021 cgacggtcac atgctcttcg ctctcatgcc agcagtcgta atcagtgacg tgggccatac
     1081 atgcgtaggc catctctgct tcggccgcca ggaacgcttc ggggctggtc gtcatgccga
     1141 tgatactcat gccccattga cgataaactt ccgattcgcc tctggtgctg aagcgtgggc
     1201 cttcgattgt aatgaatcgg ccgccgcggt ggattttccc gccggctatc tcgacggcgt
     1261 tcgtcaaggc atcgttcaac tcgacgctga acgggtttgc cgcggccagg tgagctacca
     1321 caccctcccc gaagaagctg tagtcacgca tgcccttggt gaagtcgaaa agctgatccg
     1381 ggataacgat gtcgccgggc gcaaaatcct cgcgcagcga gccgcaggcg ctgacagaaa
     1441 tcacgtactt cgcgccgagt gtcttgagcg catagatgtt ggcgcgatag gggacctcgc
     1501 tgggagtcag gacatggccg cggccatgtc gcggcaggaa cgccacgcgc cggccgtgga
     1561 gcgtgcccac ggtgatagcg tccgatggct tgccgaaagg ggtgtcgacc tccaccattt
     1621 ccacctggga caatcccggc atgttataca gaccgctgcc gccaattacg gccagttcaa
     1681 cttcgctcat ggttatctcc tgattcatat cgttcgatcg acctttttat atcataaact
     1741 acccttccgg tcgcccgtgt catctctacg ataagcctac atccgcggtt cggactcatg
     1801 tgtattttta ctcgagaatt gcccggaaac ccgatttata agtgttgtgt tatggaatga
     1861 aatgatctat agttttgcaa agcaacatcg ttttttacga cgtccatatt tgtttgaatt
     1921 atttttacgg aggaagtcta atgaaatttc tgattgcctt gctgacggcc gccactggta
     1981 tattacacct gttggtgggt tttggtatgg ttggggacga cggcatgaac tggctgcttg
     2041 tcatcaatgg agtcggctac ctggtcctgc tcgttttgtt ctggagagca tccgggaatg
     2101 gtggggcgat ccggtggatt ttgctcgcct atgcgcttat aaccttcatt ggttatttcc
     2161 tgctcaatag aggtgcaaat ttcagcttta gtaacacgat gggtctcatc gtcaaggcga
     2221 ttgagttgct cctgatactt ctattgttca tgtacaaagg atctcaggcg gccgtcgccg
     2281 gcagcccggc ggccggatcg acgcggatgg gatcgggcat cgatgcgact cgctcggcgg
     2341 gtcaggctgt gggaagtagt gcctccaagg ctgctgccgg tgcatcggcc gcggcgggcg
     2401 ccgtcacggc cgcgtcagtg tcggtagccg atcgggccgg caatgcaatc gcgggagcgg
     2461 ttgattatgt ggaggacaag gccggcgacg ctgttgacgc cgcatccgat gcagctggtg
     2521 atgtagtcgg cgcggccgag catgctgctg gggcggtggt tgacacgacc aaggatgcag
     2581 tcggttatgt gggcgataag gccggtgaag ctgttgacgc tgcggctgat gtcgctggtg
     2641 atgtagccgg cgcggtcgat catgctgctg aggcggcggt tgacacagcc ggggacgcag
     2701 tcggttatgt gggcaagaag gctggcgacg ctgttgacgc tgcggctgat gtcgctggtg
     2761 atgtagccgg tgcggccgag catgctgctg aggcggcggt tgacacagcc ggggacgcgg
     2821 ttggttatgt gggcgagaaa gccggcgacg ctgttgacgc tgcggctgat gtcgctggtg
     2881 atgtagccgg tgcggccgag cgtgctgctg aggcggcggt tgacacgacc aaggatgcag
     2941 tcggttatgt gggcgagaaa gccggtgagg cggttgacgc cgtatctgtt gatgttgaac
     3001 aagccgccga ggaaccgggc gaggacgaag tacgggccga tcttgaagag tatctgaggt
     3061 ctttcggccc ctcatcggag ttccgcaagc cgatcgagta tatcgaggga attggggcgg
     3121 tctacgggca gaagctgcga agcatcgaga tcaacacggt aatggatctt gtcctcaatg
     3181 gcgcgacccg tcgcggccgc aagcaaatcg ccgatcaatc cggaattgcc cacagcctca
     3241 ttctcacctg ggtgaatcac gttgacctgt tccgcatcaa gggcgtggcc gaagagtatg
     3301 ctgatttgct ggagcaatcg ggcgtggaca ccgtcatgga gctggcccag cgcaacccgg
     3361 ctaatctcta caagcgcatg aaagatgtta acgagc
//
LOCUS       DAOMQG010000363         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11429_length_3390_cov_6.429385, whole genome shotgun sequence.
ACCESSION   DAOMQG010000363 DAOMQG010000000
VERSION     DAOMQG010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11429_length_3390_cov_6.429385"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLC06_14545"
     CDS             complement(<1..1246)
                     /locus_tag="PLC06_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRO91713.1"
                     /translation="MNSSWTRDSFPRAAALLVLLVVSMFAMVTMASAERGAPRIVGGQ
                     EATPGEWPWQAALVKKGQDPYNGQFCGGSIIHRSWVLTAAHCIKNSAAPALTINDLDV
                     IVGIHDLGTPDPNFVRVALSEIILHPGWNYDTNDNDIALLKLASPVPARAGGGSTLPI
                     AFANTAPGNIGNLTGTLATVTGWGNRSPNGIDYPYRLHEVQVPIISNNECRGSYPGLT
                     DNMLCAGYPQGGKDSCQGDSGGPLVIYDNSAKKWIQVGVVSFGRGCALPDYPGVYARV
                     SRYESWISSYVNPPVVGANKSFVPLAVYQRYVPPPSPLVNGNFEQGPGKGWSESSSNG
                     FPIIVNNFLATGVSPHSGQWAAWLGGVYNETSVVGQSITIQSGATVLSYYYWIGSADI
                     CGYDFAYVKVKGNTVKSYNLCQN"
     gene            1638..2921
                     /locus_tag="PLC06_14550"
     CDS             1638..2921
                     /locus_tag="PLC06_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRO91714.1"
                     /translation="MKQRLSVIITLIALVAVLLTGCSIQESASAVLPVVATVAGNVAG
                     TVEQQLPTVAAQANATLQEVAQVAPTAEPVAPVTDPAPAVIEPVPVVPTSQEQQIIDL
                     YNRVNPGVVTIYMTSGSGSGFIIDAEGYIVTNNHVIAEGGEINVLFHDGDVKKAELVG
                     TDPQADIALLKVEAAPGELTALPLGDSNALQVGQSVIAIGAPFGLPNTLTTGVVSGLS
                     RAMPVEELGLGANYQTPDVIQTDAAINPGNSGGPLITMSGEVIGVNTAIESPVRANSG
                     VGFAVPANVVNVVISQLRETGAVRYPWLGIAGGTLSPDAAEELGLPRNTRGVLVSEVT
                     AGGPAAAAGLRGLDPTTNKGADIITGIDDITVLEFDDLLGYIVQQTQVGQSVTLSILR
                     DGQPQTLTLTLGERPAAPQTNEQISPNQLPIIPQP"
     gene            complement(3116..>3390)
                     /locus_tag="PLC06_14555"
     CDS             complement(3116..>3390)
                     /locus_tag="PLC06_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91715.1"
                     /translation="NTAATELNNLFGYGATAACPVDCAAQFASRGESIEFVTAILKTD
                     FLTPGGRFYNGPTLAGMAPIYVPGNPQWAEGVAAHMNLLRKNTPCP"
BASE COUNT          688 a         1100 c          982 g          620 t
ORIGIN      
        1 cattctggca cagattatag ctcttcactg tgttgccctt caccttcacg taggcaaaat
       61 cgtagccgca aatatccgca gaaccaatcc agtaatagta actgagcacg gttgcgcccg
      121 actggatggt gatggattgg ccaacaaccg acgtctcatt gtacacgccg ccaagccagg
      181 ccgcccactg accgctatga ggagacaccc cggtagccag aaaattattc acgatgatcg
      241 ggaacccatt ggaagaagat tccgaccagc ctttgcccgg cccctgctcg aagttaccat
      301 tcaccagagg cgacggcggc ggcacataac gttgatagac agccaggggc acaaaagact
      361 tattggctcc cacaaccggt ggattgacat aggagctgat ccagctctca tagcgcgaga
      421 cgcgggcata gacgccgggg tagtccggca aggcgcagcc tctgccaaag ctgacgacgc
      481 ccacctggat ccatttcttg gcgctgttgt catagatcac cagcgggccg ccactgtcgc
      541 cctggcatga gtccttgccg ccctggggat agccggcaca gagcatgttg tccgtcaacc
      601 ccggatagct gccccggcac tcgttgttgg agatgatggg cacctgaact tcatggagtc
      661 gataagggta atcgatgccg ttgggactac ggttgcccca gccggtcacg gttgcgagag
      721 ttccggtcag attgccgata ttaccaggcg ccgtgttggc gaaagcgatg ggcagcgtgc
      781 taccgccgcc cgcgcgggcc ggaacggggc tggccagctt caacaacgcg atgtcattgt
      841 cgttcgtgtc gtaattccag cccggatgaa gaataatctc gctcagtgcc acacggacga
      901 aattcggatc gggcgtgccc agatcgtgga tgccgacgat cacgtccaga tcattgatgg
      961 tgagcgccgg ggccgcgcta ttcttgatgc aatgggcggc cgtcagcacc cagctacgat
     1021 ggatgattga gccgccgcaa aactggccgt tataggggtc ctgccctttc ttgaccaacg
     1081 ccgcctgcca gggccattcg ccgggagtag cttcctggcc gccgacgatg cgcggcgccc
     1141 ctcgctcggc gctcgccatg gtcaccatgg cgaacatcga aacaaccagc aggacgagca
     1201 gcgcggccgc gcgcgggaac gaatcacgag tccacgaact gttcatattg ttctccttgt
     1261 tggtgggcgg tagcacgctt cgcatcctgg cccaaagaga caccccccac gatactcccc
     1321 ccagcctctc tcatgcgagc gaagcgcatt tcaacgcatt ataaggaaca accgaggcga
     1381 ctgtcaatat gcgtcactcc ggcatccggc acaggctgcg gtaagggaat tcacccaacg
     1441 gcctattcaa cctgactcat ccctcatcga acattaaccc ggccattaca tattttttac
     1501 gattcgatcg caaactatag agcaatattc attcgcagcc atgcgcaagc ttttgggccg
     1561 cggggttgcg aaacgtaatt gatgcaataa cgtatttatt aatgaattga tatcgtggta
     1621 ttgagacagg agttccaatg aaacagcgtt tatccgtaat catcaccctg atcgcccttg
     1681 tcgccgttct gctgaccggc tgcagcattc aggagagcgc gtcggccgtg ttgccggtcg
     1741 tcgctaccgt cgccggtaat gtggccggca ccgtcgagca gcagctgccc accgtcgccg
     1801 cccaggccaa cgccaccctt caggaagtgg ctcaggtagc cccgacggcc gagccggtgg
     1861 cccccgtcac cgaccctgcg ccggcggtca tcgagccggt gcccgtggtc ccgacctcgc
     1921 aggagcagca gatcatcgac ctctataacc gggtcaaccc cggcgtggtc accatctata
     1981 tgaccagcgg cagcggctcc ggcttcatca tcgacgccga gggctatatc gtcaccaaca
     2041 accacgtcat cgccgagggc ggcgagatca acgtcctgtt ccacgacggc gacgtgaaga
     2101 aggccgaact ggtgggcacc gacccgcagg ccgacatcgc cctgctgaag gtcgaggccg
     2161 cgcccggcga attgaccgcc ctgccgctgg gcgactccaa cgccctgcag gtgggccaga
     2221 gtgtcatcgc catcggcgcg ccgttcggcc tgcccaatac cctgaccacc ggcgtcgtca
     2281 gcggcctgag ccgggccatg ccggtcgagg agctgggcct gggcgcaaat taccagacgc
     2341 ccgatgtcat ccagaccgac gcggccatca accccggcaa ctccggcggc ccgctgatca
     2401 ccatgtcggg cgaggtcatc ggcgtcaaca cggccatcga gtcccccgtg cgcgccaaca
     2461 gcggcgtcgg cttcgccgtg ccggccaacg tggtcaatgt cgtcatcagc cagctgcgcg
     2521 agacgggcgc ggtgcgctac ccgtggctgg gcatcgccgg cggcacgtta agccccgacg
     2581 cggccgagga gctgggactg ccgcgcaata cccgcggcgt gctggtcagc gaagtgacgg
     2641 cgggcggccc ggcggccgcg gccggcctgc gcggcctcga cccgacgacc aacaagggcg
     2701 ccgatatcat caccggcatc gacgacatca ccgtgcttga attcgatgac ctgctgggct
     2761 atatcgtgca gcagacccag gtcggccaga gcgtgacgct ttccatcctg cgcgacggcc
     2821 agccacagac gctgaccctg accctgggcg agcgcccggc tgccccgcag acgaacgagc
     2881 aaatatcccc caatcagctg ccgattatcc cacagccgta ggccaacctt actttcaccg
     2941 gcgggcttcc cgaaagaagc cgggaagctc gccttgtcct cctccggaag aggcccgctc
     3001 accagctatt gagcgggcct ttttgtttat atcagtcggc caaatccaga tacgagatac
     3061 aaatccggaa gcgctcttca ctcgtatctc gtatctctta tttcgcatct cgtatctatg
     3121 gacagggcgt atttttccgc aacaggttca tatgggcggc tactccctcg gcccattggg
     3181 ggttgccggg gacgtagatt ggcgccatac cggcaagcgt cggcccgtta tagaagcggc
     3241 cgcccggagt cagaaaatcg gtcttcagga tggcggtcac aaattcaatc gactcccccc
     3301 gcgaggcgaa ttgcgcggcg caatcgacgg ggcaggcggc cgtcgcgcca tagccaaaca
     3361 ggttgttcag ctccgtcgcc gccgtgttat
//
LOCUS       DAOMQG010000364         3376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11489_length_3376_cov_9.791930, whole genome shotgun sequence.
ACCESSION   DAOMQG010000364 DAOMQG010000000
VERSION     DAOMQG010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11489_length_3376_cov_9.791930"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLC06_14560"
     CDS             <1..923
                     /locus_tag="PLC06_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRO91716.1"
                     /translation="NVNAPTIAQFVASPSQVTVGQCVTLQWDVQGQVNRVTISNQFRV
                     IWDNAPSRGNTQDCTGGQPTTIEYRIQANGPGGTSQALDHVSVVNPATATPVPTAPPS
                     QPIIDSFNITPAQIETGQCVQITWSTSGATWLVRLLRNNVLVLDNAALAGSAQDCLAF
                     AGSYSYQLVASTAGGQSTSRSQSVLVTQAPPTNPLAGQTYTLDKLNGAPILTNTNMTI
                     TFSSDGTLNGFSGCNNYSGRYTANNGQITVSEINGTNMFCVDPPGVMEQEGAYINALR
                     AAASFEIPGMSAQLIIRNAATQEILHYIVK"
     gene            complement(985..2058)
                     /locus_tag="PLC06_14565"
     CDS             complement(985..2058)
                     /locus_tag="PLC06_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRO91717.1"
                     /translation="MEGELMDVTGPTDNDENGHAATGNAHAHMPNYSIVVYDTQTIRS
                     FDCQSVDEVLEQADLSRVNWVTVRDVHDEAELTRLLTYFGIHSDVLPSLLDEVQTEFE
                     VEYDDCLYLEYMLPYLDRQPRRLVQSRGSFILTTQALILYEHQLHGLFARTRRRALNK
                     LTHIMEYGPDYLLYLLLRGVIVEHFQRGFKHLAAELEELEDRVLIGRGREEVYRTILI
                     AREGIKPWNDPLLEMENFLEYVEDAESKFITEKAAKYFTKSLLREVETLLGSYDRLRV
                     MLKEVMDLHMGNVERNTSRVNQLLTVIATIFLPITFIASIYGMNFEYMPELEQPWGYP
                     AVLLLMLGVALSLILFMKRRRWF"
     gene            complement(2082..>3376)
                     /gene="argS"
                     /locus_tag="PLC06_14570"
     CDS             complement(2082..>3376)
                     /gene="argS"
                     /locus_tag="PLC06_14570"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRO91718.1"
                     /translation="QVECVSANPTGPITLGRTRGGVIGDTLHRVLEAAGYDVTMEYYY
                     NDAGRQVTLLGESVKVRYLQQLGQAAELLEEHYQGDYIIDLAQELIVEHGDALVDVPT
                     DVFSEYARDQISRQQKATLARVGIVFDVYFREQSLYETGAVWRTLEVLRERGYIYEKD
                     GAQWFRSTEFGDDKDRVVVKADGEPTYRLPDMAYHWDKAQRGFDLVVDIFGPDHHATA
                     PQVLMGVRALGYNSDFVHTLLHQIVTLVRGGDTVKMSTRRGQYLTLDELVDEVGADPI
                     RYFMISRSANSPVDFDLDLAMEQSDKNPVYYIQNAHVRCAGIFRKWVEAGFAEDADEG
                     ADLSLLTHERELAFLRKALELGDVVETIATTYEPHRLAFYAYDLAALFHPAYEECRVL
                     HSEVPEPVRLARLRFYRAAKAVFARVLDLMGMTAPDVM"
BASE COUNT          665 a         1064 c         1023 g          624 t
ORIGIN      
        1 ccaacgtcaa cgccccgacc atcgcccagt ttgtggcctc gccgtcgcag gtcactgtcg
       61 gccaatgtgt gacgctgcaa tgggatgttc agggccaggt caatcgggta accatcagca
      121 accagttccg cgtgatctgg gacaatgccc cgtcgcgcgg gaacacgcag gattgcaccg
      181 gcggccagcc gacgacgatt gagtatcgca tccaggccaa cggccccggt ggcaccagcc
      241 aggcgctcga ccacgtgagt gtcgtcaacc cggccacggc cacccctgtg ccgaccgcgc
      301 cgccgagcca gccgatcatc gactccttca acatcacgcc ggcgcagatc gagaccggcc
      361 agtgcgtcca gatcacctgg agcaccagtg gcgcgacatg gctggtgcgc ttgctgcgca
      421 acaacgtgct ggtgctcgat aacgccgcgc tcgccgggtc ggcgcaggat tgtctcgcgt
      481 ttgccgggtc atacagctac cagcttgtgg ccagcaccgc cggcggccaa tccacgtcgc
      541 gaagccagtc cgtgttggtg acccaggcgc cgccgacgaa cccgctggca ggtcagacat
      601 acactctgga caagctgaac ggcgcgccga ttctgaccaa caccaatatg actatcactt
      661 ttagcagcga cggcacgctg aacggcttta gcggctgcaa caactacagc gggcgatata
      721 cggccaacaa cggccaaatc acggtgagtg aaatcaacgg gaccaacatg ttctgcgtcg
      781 atccgccggg cgtgatggag caggaagggg cctatatcaa cgcgttgcgc gccgcggcgt
      841 catttgaaat cccgggcatg tcggcccagc taatcatccg caacgcggcc acccaggaaa
      901 tcctgcacta tatcgtgaaa tagagagact gcgggggcga gtttcaagct cgtccccgca
      961 aaatgacagg gtgcgtgaaa caggtcagaa ccagcgccgg cgcttcatga aaaggatcaa
     1021 gctcagggcc acccccagca tcaggagcag caccgccgga tagccccacg gttgctccag
     1081 ttcgggcatg tactcgaagt tcatgccgta gatgctggcg atgaaagtga tgggcaggaa
     1141 gatggtcgcg atcaccgtta atagctggtt gacgcggctg gtgttgcgtt ccacatttcc
     1201 catgtggaga tccatcactt ccttcagcat aacccgcagc cgatcatagc tgccgagaag
     1261 cgtctcgacc tcgcgcagca gcgacttggt gaaatatttg gccgcttttt cagtgatgaa
     1321 cttcgattcg gcatcttcca catattccag aaagttttcc atctccagca acggatcgtt
     1381 ccacggcttg atgccttcgc gggcgatcag gatggtgcga tagacctctt cgcgccctcg
     1441 gccgatgagc acgcgatctt ccagctcttc cagctcggca gccagatgct tgaagccgcg
     1501 ctggaaatgc tccacgatga cgccccggag cagcagatag agcaggtaat cggggccata
     1561 ctccatgatg tgggtcagct tgttgagggc gcggcggcgc gtgcgcgcga aaaggccgtg
     1621 cagctgatgc tcataaagga tgagggcctg ggtcgttagg atgaaggagc cgcgactctg
     1681 caccagccgc cgcggctgcc ggtccagata ggggagcata tactccagat agaggcaatc
     1741 gtcgtattcg acttcgaatt ccgtctgcac ttcatcaagc aggctgggca atacgtcgct
     1801 gtggatgccg aagtaggtca gcagccgggt cagctcggct tcgtcgtgga cgtcgcgcac
     1861 cgtcacccag ttgacgcggg acagatcggc ctgctccagc acctcgtcca cgctctggca
     1921 gtcgaacgag cggatcgttt gggtgtcata gacgacaatg gagtaattgg gcatgtgggc
     1981 gtgcgcgttg cctgtggcgg catgcccatt ctcgtcgttg tctgtggggc cggtcacgtc
     2041 catcagctct ccttccatgc attggctccg ttggcgaccg gctacatcac atccggcgcg
     2101 gtcatgccca tcagatcgag tacgcgggcg aagacggcct tcgccgcgcg atagaaacgc
     2161 agccgcgcca gccgaaccgg ctccggcacc tcgctatgca gcacgcggca ctcctcatac
     2221 gcgggatgga acagcgccgc cagatcatag gcatagaaag ccagccggtg cggctcatag
     2281 gtcgtggcga ttgtctcaac tacgtcgccc agttccagcg ccttgcgcag aaaagccagc
     2341 tcgcgctcgt gggtcagcaa gctcaggtcg gcgccttcgt cggcatcctc ggcgaagccc
     2401 gcctcgaccc atttgcgaaa gatgcccgcg caacgaacgt gggcgttctg gatatagtag
     2461 accgggttct tgtccgactg ctccatggcc agatcgagat caaaatcgac cgggctattg
     2521 gccgagcgcg agatcatgaa atagcggatc gggtcggcgc ccacctcgtc aaccagttca
     2581 tcgagcgtca ggtattggcc gcggcgcgtg ctcatcttga ccgtgtcgcc gccgcgcacc
     2641 agagtcacga tctgatgcag cagcgtatgc acaaagtcgg agttatagcc cagcgcccgc
     2701 acgcccatca acacctgggg ggcggtggcg tggtggtcgg ggccgaagat gtccaccacc
     2761 aggtcgaagc cgcgttgagc cttgtcccaa tggtaggcca tgtcgggcag gcggtaggtc
     2821 ggctcgccat cggccttgac gaccacgcgg tccttgtcgt cgccgaactc ggtcgaccgg
     2881 aaccattgcg cgccgtcctt ctcatagatg tagcccctct cgcgcaaaac ttccagtgtc
     2941 cgccacaccg cgccggtctc atagaggctt tgctcgcgga aatagacgtc gaagacgatg
     3001 ccgacgcggg ccagcgtcgc cttctgctgc cgcgagattt ggtcgcgcgc gtattcggag
     3061 aacacgtccg tcggcacatc gaccagcgcg tcgccgtgtt cgacgatcag ctcctgggcc
     3121 aggtcgatga tgtaatcgcc ctggtagtgc tcctcaagca actcggccgc ctggcccagt
     3181 tgctgcaaat agcgcacctt gaccgattcg cccagcaagg tgacctgtcg cccggcgtcg
     3241 ttgtagtaat actccatggt cacgtcgtag ccggcggctt ccagcacacg atggagcgtg
     3301 tcgccgatga cgccgccgcg ggtgcggccg agcgtgatcg gcccggtcgg gttggccgac
     3361 acgcattcca cctgcg
//
LOCUS       DAOMQG010000365         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11703_length_3324_cov_8.730193, whole genome shotgun sequence.
ACCESSION   DAOMQG010000365 DAOMQG010000000
VERSION     DAOMQG010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11703_length_3324_cov_8.730193"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1075
                     /locus_tag="PLC06_14575"
     CDS             227..1075
                     /locus_tag="PLC06_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRO91719.1"
                     /translation="MDILLQPIQYDFIVRALMAAGIVGVVCSILGVYIVLRGMAFLGD
                     AMAHTILPGVVVAFLLGWPLAVGALIMGMLTALGIGALTERTALKEDTAIGVIFAGLF
                     ALGVAMLSLRGNYSVDLAHFLFGNLLGVSSADLILIAVLGAAVLLLIFLFYKEFLVLS
                     FDPLLAETLRLPTRFLNYLLLILIAVTIVVALQVVGVALMLAILVTPAATASFLTRRL
                     PSMMVVSAAIGVFSGVVGVYASYYLNIASGPAVVLVATLIFLIVLTATSARRRSASAT
                     VPARAR"
     gene            1072..1503
                     /locus_tag="PLC06_14580"
     CDS             1072..1503
                     /locus_tag="PLC06_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRO91720.1"
                     /translation="MNGDLYSALEARGLRLTAARRAIIDTLVELGGHVTADELTDLLR
                     GRETGIGRMTIYRTLDLLCDLGLLRPAYQGTGAAHYILLHDGHHHHLVCSGCSRVIEF
                     DDCGLDELSTSVARRYGFRVEGHLLELYGVCADCRGAAAEG"
     gene            complement(1523..2398)
                     /locus_tag="PLC06_14585"
     CDS             complement(1523..2398)
                     /locus_tag="PLC06_14585"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRO91721.1"
                     /translation="MKILITGGSGYLGRHLVRRLAKGAGTFRYTCFSADPLGLPQGVT
                     LDLRDGAAVARFVADFAPDAIIHTAGSNRTPDMAAVIVEGTRHVTAAAAAVGARLIHL
                     STDSVFDGAHAPYDEAATPAPLNAYGRAKATAEAIAAGHPDAVIVRTSLIYGLEEMDN
                     GTAWMAAALREGRPVTLFTNQRRNPVWVETLCAACLALAERLSSPAGNYRGVLNVAGR
                     QVMTRAAFGLRMLDWWGVAERATLSLGLDESGQWPPDCTMDVNRAESLLPVTLWGVDE
                     LLARAGRDDLPQARA"
     gene            complement(2475..3170)
                     /gene="rnc"
                     /locus_tag="PLC06_14590"
     CDS             complement(2475..3170)
                     /gene="rnc"
                     /locus_tag="PLC06_14590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRO91722.1"
                     /translation="MEDLVELSERLGVEFNDYSLLSRALTHRSYLNENPGSSFEDNER
                     LEYLGDAVIDFIVAGYLYHRFPEMDEGELTALRAALVRAETLAQFARAIDLGRYLRLG
                     YGEEESGGRDRTPLLCATFEAVIGAIYLDRGLERARLIVEGHIRPMLVDIRAGSLHKD
                     AKSEFQVWAQARFNRTPHYRVIDSEGPDHAKTFTVRVMVGDDSWGEGRGRSKQAAAQA
                     AAAEAMARAESEE"
BASE COUNT          517 a         1110 c         1059 g          638 t
ORIGIN      
        1 ccgagcgctt cgaccggatc atgctcctga atcgccggat catcgccttt gaatcgccgg
       61 ccgtcgccct gcgcgccgag aatctgctca gcgcctacgg aggacggtga taattacgaa
      121 ttacgaatta cgaataaaga ggtgggttgt cagcggtcgg cggtcgaccg tggtcggcga
      181 tcactcttca ttcgtcactc gtcactcgtc attcatcact taaactatgg atatcctgct
      241 ccaacccatt caatatgact ttatcgtccg cgcgctgatg gcggcgggga ttgtgggcgt
      301 ggtctgctcg atccttggcg tctatatcgt gctgcggggc atggcctttc tgggcgacgc
      361 gatggcccat actatcctgc ccggcgtggt ggtcgccttc ctgctgggct ggccgctggc
      421 cgtgggggcg ctcatcatgg gtatgctgac ggcgctgggt atcggcgcgc tgacggagcg
      481 cacggccctg aaagaagata ccgccatcgg cgtcatcttt gccgggctgt tcgccctggg
      541 ggtggccatg ctgtcgctgc gcggcaacta cagcgtcgac ctggcccact tcctgttcgg
      601 caatctgctg ggcgtctcat cggccgacct gatcctgatc gccgtactgg gcgcggccgt
      661 cctgctgctc atcttcctgt tctataagga gttcctcgtc ctgtcattcg atcccttgct
      721 ggccgagacg ctgcgcctgc cgacgcgctt cctgaactac ctgctgctca tcctcatcgc
      781 cgtgaccatc gtcgtcgccc tgcaggtcgt gggcgtggcg ctgatgctgg ccattctggt
      841 gacgccggcg gccactgcgt cgttcctgac ccgtcgcctg ccgtcgatga tggtcgtctc
      901 ggccgccatc ggcgtgttct ccggtgtggt cggggtctac gcctcgtatt acctcaacat
      961 cgcttcgggg ccggcggtgg tgctggtggc gacgctcatc ttcctgatcg tgctgaccgc
     1021 gacctcggcc cgcaggcgca gcgcgtcggc gaccgttccg gcgagggcaa gatgaacggc
     1081 gatctgtata gcgcgcttga agcgcgaggg ctacggctga cggccgcacg gcgggcgatc
     1141 atcgacacac tggtggagtt gggcggccat gtgaccgccg acgagttgac cgacctgttg
     1201 cgcgggcgcg agaccggcat cggccgcatg accatctatc gcacactcga cctgctgtgc
     1261 gatctcggcc tgctgcggcc cgcctatcag gggacgggcg cggcccacta catcctgctc
     1321 cacgacggcc accaccacca tctggtctgc tccggttgca gccgggtcat cgagttcgac
     1381 gactgcggcc tggacgagct atcgacgagc gtcgcccggc gctacggctt tcgcgtcgag
     1441 gggcacttgc tggagttata tggggtgtgc gccgattgcc ggggcgcagc tgccgaaggc
     1501 tgatggtcgg cgatccggcc ggttatgccc gcgcctgcgg cagatcatcc cgcccggcgc
     1561 gggccaacag ttcatccact ccccacaacg tcaccggcag caggctttcg gcccggttca
     1621 cgtccatcgt gcagtccggc ggccattgcc cactctcgtc cagccccagc gagagcgtcg
     1681 cccgctccgc cacgccccac cagtcgagca ttcgcagccc aaacgccgcc cgggtcatca
     1741 cctgccgccc ggccacattg agcacgccgc gataattgcc cgccggagag gacaagcgtt
     1801 cggccagcgc caggcacgcc gcgcagagcg tctccaccca taccgggttg cgccgctggt
     1861 tggtgaagag tgtcaccgga cgcccctcgc gcagcgcggc cgccatccag gccgtgccgt
     1921 tgtccatttc ctccagcccg taaatgagcg aggtgcgcac gatgacggcg tcgggatgcc
     1981 cggccgcgat tgcttcggcg gtggccttgg cccggccata ggcgttgagc ggcgcgggcg
     2041 tggccgcctc gtcataaggg gcgtgcgcgc cgtcgaacac ggagtcggtt gagaggtgaa
     2101 tcagccgcgc gcccacggcg gcggccgcgg cggtcacatg gcgcgtcccc tccacgatga
     2161 cggccgccat gtcgggggtg cgattggagc cggcggtgtg gatgatggcg tcgggggcga
     2221 agtcggccac aaagcgggcc acggccgcgc cgtcgcgcag gtcgagcgtc acgccctgcg
     2281 gcaggccgag cgggtcggcc gagaaacaag tgtagcgaaa tgtcccggcc cccttcgcca
     2341 accgccgcac cagatgccgc cccaagtaac cactgccgcc agtaatgagt attttcatat
     2401 tattggggtt aggaaccggg gttagaaacc gggtttttcg gaaaaaaccc ggtttccttc
     2461 tgtatctttg cgccctattc ttctgactcc gcccgcgcca tcgcctcggc cgcggcggcc
     2521 tgcgccgcgg cctgcttgct gcggccgcgc ccctcgcccc agctgtcgtc gccgaccatg
     2581 acgcgcacgg tgaaggtctt ggcgtgatcc ggcccctcgc tgtcgatgac gcgatagtgg
     2641 ggcgtccggt tgaaacgagc ttgcgcccac acctggaact cgctcttggc gtccttgtgc
     2701 agcgacccgg cgcggatatc caccagcatc ggtcgaatgt gcccctcgac aatgaggcgc
     2761 gcccgctcca gcccccggtc gagatagatc gcgccgatga ccgcctcgaa agtggcgcac
     2821 agcaagggtg tccggtcgcg gccgccgctc tcttcctcgc catagcccag acgcagatag
     2881 cggccgaggt cgatggcgcg ggcgaactgg gccagcgtct cggcccgcac cagcgccgcc
     2941 cgcagcgccg tcagctcgcc ctcatccatc tccgggaagc gatgatagag ataaccggcg
     3001 acgatgaagt cgatgacggc gtctcccaga tactccagcc gctcgttatc ctcgaagctg
     3061 gagccggggt tttcgttcag atacgagcga tgggtcagcg cgcgggagag cagcgaatag
     3121 tcgttgaact ccacgccgag gcgctcgctc aattccacta ggtcttccat gtgattctcg
     3181 gaccaggaga atccacagat tccacagatt ggcatcttac atctgtgaaa tctgtgcaat
     3241 ctgtggattt cttttattca aactcgcgga tgaccgtgac ggcattgtgg ccgccgaagc
     3301 caaaggcgtt attgaggcaa acgc
//
LOCUS       DAOMQG010000366         3308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11763_length_3308_cov_6.207808, whole genome shotgun sequence.
ACCESSION   DAOMQG010000366 DAOMQG010000000
VERSION     DAOMQG010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11763_length_3308_cov_6.207808"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..462)
                     /locus_tag="PLC06_14595"
     CDS             complement(157..462)
                     /locus_tag="PLC06_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRO91723.1"
                     /translation="MEHPIYRVTDYKIVGPYTLRVSFDDATSQVIDFQPVLFGHYFGP
                     LRDQKMFEQVEIDPEVHTLVWPNGADFDPLTLHNWPEYVDELIALTQSWKERENELA"
     gene            complement(480..869)
                     /locus_tag="PLC06_14600"
     CDS             complement(480..869)
                     /locus_tag="PLC06_14600"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HRO91724.1"
                     /translation="MNDPLFEELLESVREGAAIIRGEARPSRLFKINNQVSGVEMPEI
                     SRFYGIIIRMFVEQNAPHHRPHFHAYYQNRSAVFGLDPIELISGQLSPKERRLVEAWA
                     ELHNEELGENWSRIRAGETVLKIAPLK"
     gene            complement(898..3126)
                     /locus_tag="PLC06_14605"
     CDS             complement(898..3126)
                     /locus_tag="PLC06_14605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRO91725.1"
                     /translation="MLRFSDFSLLPEVDELVRQLVGEQSGLVVVAGLDARPAAEPVSE
                     PSLHFLPSGRATIFRVLAGELLEAHPDARGLFVAEDRDILLTARRFRRNLQQVQVKPP
                     LTYAEALGDAGQFDVIIVDRLSPDNLPSLLAAAQAGALVLSQMDTLFHSQAVVGALAD
                     LCPAPGDLAALRWVLSVQRLPTLCPICKQPVVLSADQVARLEWLRRRFPALEADGGSE
                     FYAPGTCDTCNNSGRRGDVAVFDFLRLDGTGGDGPARPSAMPMEAYIRVLAERGLLAL
                     SDVLEFESRQLHRTYRLLAATQRRLNTTTAELERNTIKLDTTGRLLKQRTRELVALEG
                     IGRTLITWSDLRELGDRVLKTALELSKADRGILYYIRSAQWGQILAARGWREAQIEMG
                     ISCGTIHATLSSEDPTAYEGTPPGLTLLADTSPSRAGQAIPLRAHGIPVGMMLIQSTR
                     KPRFTQGEVAILQTLAGYAAVAMQRAEFIDQLRIKISELEEAQDELAQKERLERELEL
                     ARQVQLSMIPRAFPAVPGITFAASYEPARQVGGDFYDVIPLDDGRIALIIADVADKGL
                     PAALYMAQTRSLIQAVARHSDTPAETLQRVNGLLRELGEQDMFVTVFYAIMDKERRRL
                     TYVRAGHEHPFLVRDGRVLPLGGRGMVLGLFDNPAFMALEESLELQAGDKLVLYTDGL
                     TDVINPAGEMPAHDRLAALVAAHAVEPPAGMCRALFADLTAWRGSAAQFDDMALLVVG
                     VE"
BASE COUNT          613 a         1003 c         1071 g          621 t
ORIGIN      
        1 ccgatgagat cccctacgcg ccggcgtgct acttctatga tcagcagccg cacacgttcc
       61 ggggggacgg ggacgcgctg ttgatggagc gggtggctga gttttttggg cgtcattaga
      121 aaccgggttt cttccgggga attcggcttc cgggttttat gcaagctcgt tttcgcgctc
      181 tttccagctt tgagtcagag cgattaattc atcaacgtac tcaggccagt tatgcaaggt
      241 gagcgggtca aaatctgctc cgttcggcca gaccagggtg tggacttcgg ggtcgatctc
      301 aacctgctcg aacatctttt gatcgcgtag cggtccgaag tagtggccga agagcaccgg
      361 ctgaaaatca attacctggc ttgtggcatc atcaaaagac actcgcaggg tatagggtcc
      421 tacgatctta tagtctgtga cgcgatagat gggatgttcc attgtcttca ccctctccct
      481 tatttgagcg gggcaatctt caatactgtt tcaccggctc gtattctact ccagttttca
      541 ccaagttctt cattatggag ttctgcccag gcctctacca ggcgacgctc ctttggtgac
      601 agttggccgg aaatcaattc tatcggatcg agtccgaaaa cggccgacct gttctgatag
      661 taggcatgga aatgcggccg atggtgcggg gcattttgct ccacgaacat acggataata
      721 ataccataga aacgactgat ctcaggcatc tcgactcctg acacttgatt gttaatcttg
      781 aatagacgtg atggccgagc ttcgccccgg atgatggcgg cgccttcacg cacactttcc
      841 agcagttcct caaacaacgg atcgttcata gacatacacc ctgttcaatg acacaggcta
      901 ctccaccccc accaccagca acgccatatc atcaaactgg gccgcgctgc cgcgccaggc
      961 ggtcaggtcg gcgaagaggg cgcggcacat tccggccgga ggctcgacgg catgggccgc
     1021 caccagcgcc gccagccggt catgggccgg catctcgccg gcggggttga tgacgtcggt
     1081 caggccgtcg gtatagagca ccagcttgtc gccggcttgc agttccagcg attcttccaa
     1141 cgccatgaag gccgggttgt cgaagaggcc aagcaccata ccgcggccgc ccaggggcag
     1201 cacccggccg tcgcgcacca ggaacgggtg ttcgtgcccg gcgcgaacgt aggtcagccg
     1261 ccgccgctcc ttgtccatga tggcgtagaa gaccgtgacg aacatatcct gctcgcccag
     1321 ttcccgcagc aagccattga cgcgctggag agtctcggcc ggtgtgtcgg agtggcgggc
     1381 cacagcctga atgaggctgc gcgtctgggc catatagagg gcggcgggca agcccttgtc
     1441 ggccacatcg gcgatgatca gggcaatccg gccgtcgtcg agcgggatga cgtcgtagaa
     1501 gtcgccgccc acctgccgcg caggctcata ggacgcggcg aaggtgatgc ccggaaccgc
     1561 cggaaaagcg cgggggatca tgctcaattg cacctggcgg gccagctcca gctcgcgctc
     1621 cagccgctcc ttttgggcca attcgtcctg ggcctcctcc agttcactaa tcttgattct
     1681 cagctggtca atgaattcgg cgcgctgcat ggccacggcg gcatagcccg ccagcgtttg
     1741 caggattgcc acctcgccct gggtgaaacg cggtttgcgc gttgactgaa tgagcatcat
     1801 gcccacgggg atgccgtggg cgcggagcgg gatggcttgc ccggcgcgcg agggcgatgt
     1861 gtcggccaac agcgtcagtc ccggcggcgt gccctcatag gcggtcgggt cttcgctcga
     1921 cagcgtcgcg tggatcgtcc cacaggagat gcccatctcg atttgcgcct cgcgccagcc
     1981 ccgcgcggcc agaatttgac cccattgggc cgagcgaatg tagtagagga tgccgcggtc
     2041 ggccttgctc agctccagcg ccgtcttcag cacgcggtcg cccagctcgc gcagatcgct
     2101 ccaggtgatg agcgtccggc cgatcccttc cagggcgacc agctcgcggg tgcgctgctt
     2161 caacaggcgg ccggtcgtgt cgagcttgat cgtgttgcgc tccagttcgg ccgtggtcgt
     2221 gttcaggcgg cgctgggtgg cggccagcag ccggtaggtg cggtggagtt gccggctttc
     2281 gaactccagc acgtcggaca aggccagaag cccccgttcg gccagcacac ggatataggc
     2341 ttccatcggc atggcgctcg gccgcgccgg cccatcgccg cccgtgccgt cgagccgcag
     2401 gaagtcgaat acggccacgt cgccgcggcg gccgctgttg ttgcaggtgt cacatgtccc
     2461 cggcgcgtag aactcgctgc cgccgtcggc ctccagcgcc ggaaaacgac gccgcaacca
     2521 ttcaagccgg gccacctggt cggccgacag gacgacgggt tgcttgcaaa tggggcacag
     2581 cgtcggcaac cgctggacgc tcagcaccca gcgcagcgcc gcgaggtcgc ccggtgcggg
     2641 gcacaggtcg gccagcgcgc ccaccaccgc ctgggaatga aacagggtgt ccatctgcga
     2701 cagcaccagc gccccggcct gcgcggcggc cagcaacgag ggcaggttgt cggggctgag
     2761 gcggtcgacg atgatgacgt cgaattggcc ggcatcgccg agggcttcgg cgtaggtcag
     2821 cggcggtttc acctgcacct gttgcaggtt gcggcgaaaa cggcgcgccg tcagcaggat
     2881 atcgcggtct tcggccacga acaggccgcg cgcgtcgggg tgggcctcca gcagctcccc
     2941 ggccagcacg cgaaagatgg tggcacggcc gctgggcaga aagtggagac tcggctcgga
     3001 gacaggctcg gccgccggtc gcgcatccag cccggccaca acgaccagcc cggattgctc
     3061 gccgacgagt tgtcgaacca gctcatctac ttcgggcagc agcgaaaagt cggagaagcg
     3121 gagcatgggg ggagtatagg ggagaaatta cgaattacga attacgaatt acgaatggat
     3181 cggcggtcag tggggcagtg ggtcagtggg tcagtgggtc agtgggtcag tgggtcagtg
     3241 ggtcagtggg tcagtgttat gccctgaaaa aagtggacag taagttaagg tgtaaccagt
     3301 aaggcctc
//
LOCUS       DAOMQG010000367         3277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11913_length_3277_cov_10.755742, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000367 DAOMQG010000000
VERSION     DAOMQG010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11913_length_3277_cov_10.755742"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1256
                     /locus_tag="PLC06_14610"
     CDS             75..1256
                     /locus_tag="PLC06_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO91726.1"
                     /translation="MIKAQRFALLVLALVGAAVLAACQAGGGGGDTIKVGLNLELTGS
                     IPKVGEHSRVAAEMYAEEVNAAGGLDVGGKKYNIELVVEDNNGTAEGAAAAANKLVTQ
                     DNVLVLVGPNASVAAVPAGEVANNLATPMISPWSTNPNTTLNRPWVFRAPFLDPFQGP
                     VVANFATEQFGAEKACVLFDVASDYPKGLADNFKSAWEGLHGPGSVVAFESFTTGDQD
                     FSAQLTKIKATDCQVLFTPQYYSEVPLIVEQAQDLGITLPIVGSDSWGDPQLLELCGD
                     ACEGFFYSTHYAASGATGATKEFIDKFQARHGEIPSDVGALTWDSMQIVTQAIKNCGS
                     LAGSLEENRTCVRDGMAAIKDFEGITGTMTFDEQGDPIKCAVIVQIQGGEAVFYKSVC
                     P"
     gene            1374..2300
                     /locus_tag="PLC06_14615"
     CDS             1374..2300
                     /locus_tag="PLC06_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO91727.1"
                     /translation="MDTFLQHLLNAIQWGSFLALIACGYTMVYGVLLMINFAHGDIFM
                     VGAYLGFFIGTFLLGGFALRLPIELPLWAVVLLTLVMTMSLTALVGVTIERVAYRPLR
                     KKGAQRLYLVITALMFGLLLEYGNLALLGARDRQFPQLFPKQVYTFGGLTITNVKLMV
                     ILLSILTMVTLALLVQRTKWGMAMRAISYDRLAVPLMGVSTDTIIIMTFVVGSALAAL
                     AGVMYGTAFPILSYDMGLLVGWQAFIAAVLGGIGSIRGAMVGGYILGFTQVFVVAFLP
                     STYRDLITFTLLLVILVVRPTGLFGVARRTKV"
     gene            2310..>3277
                     /locus_tag="PLC06_14620"
     CDS             2310..>3277
                     /locus_tag="PLC06_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO91728.1"
                     /translation="MRRYGLTILAIVVFLLTVIPSWLGLYSGYIQFLLAVIGINIILT
                     VSLNLINGYMGEFSVGHAAFMGVGAYVASLVTVSLFGENSLLGDVNLPPALSLLVFPV
                     ALLIGGVAAALFSLLVSIPSFRTRGDYLAIITLAVNFIFKSLVENVDAIGGPRGFMGM
                     GGVLRVMGGVADIPWLLIWTFLSMVFTIYIIRNFVTSTIGKSVVAIRDDEIAAEVMTV
                     NTRRLKIIAFMLGCGLAGVAGGLHAHLLGFINPSSYFLLQSTLILVMVYLGGIGSISG
                     SIISAIAFTVLLELLRPLDLFRWVLTPLLLIGLMLFRPTGIMGDREL"
BASE COUNT          557 a         1048 c          965 g          707 t
ORIGIN      
        1 catttcttgc gccatcaacc acatttcgat aaagtttctc tccttaaaaa ccagttgctt
       61 ggaggtatgg atcgatgatc aaagcacaga gatttgcgct acttgttctg gcgcttgtcg
      121 gcgcggcggt attggccgcc tgtcaggcgg gaggcggagg cggtgatacc atcaaggtcg
      181 gccttaatct cgaattgacc ggcagcatcc ccaaggtcgg cgagcactcg cgcgtcgcgg
      241 ccgagatgta cgccgaagag gtcaatgccg cgggtggtct ggacgtcggc ggaaagaagt
      301 acaacatcga actggtcgtc gaggacaaca acggcacggc cgaaggcgcg gccgccgcgg
      361 ccaacaagct cgtcacccag gacaatgtgc tggtgctggt cggcccgaac gccagcgtgg
      421 ccgccgtgcc cgcgggcgag gtggccaaca acctggcgac gccgatgatc agcccgtggt
      481 cgaccaaccc caacaccacg ctcaaccgcc catgggtctt ccgcgcgccg ttcctcgacc
      541 cgttccaggg gccggtcgtc gccaattttg ccaccgagca gttcggcgcc gagaaggcct
      601 gcgtcctctt cgacgtcgcc agcgattacc ccaaggggct ggccgataac ttcaagagcg
      661 cctgggaagg gctgcacggt cccggctcag ttgtcgcctt cgagtcattc accaccggcg
      721 atcaggattt cagcgcccag ttgaccaaga tcaaggccac cgactgccag gtgcttttca
      781 cgccgcagta ctacagcgaa gtgccgctca tcgtcgagca ggcccaggac ctgggcatca
      841 ccctgccgat cgtgggtagc gactcctggg gcgatcccca attgctggag ttgtgcggcg
      901 atgcctgcga gggcttcttc tacagcaccc actacgccgc ttccggcgcg accggcgcga
      961 caaaggagtt catcgacaaa ttccaggctc gccacggcga aatccccagc gacgtcggcg
     1021 cgctgacctg ggactcaatg caaatcgtca cccaggcgat taaaaattgc ggctcactcg
     1081 ccggcagtct ggaagagaac cgcacgtgcg tccgggatgg aatggccgcc atcaaggact
     1141 tcgagggcat caccggcacg atgaccttcg acgagcaggg cgatccaatc aagtgcgcgg
     1201 tcatcgtcca gattcagggc ggcgaggctg ttttctacaa gtcggtttgc ccgtaggccc
     1261 atagctgcac agagtagcgc tttaactaac gaatagcacg attgagtgcc cgtcatgcgc
     1321 cgtcgtgcta ttcgttgctg ttaaccaacc ggaaatccga cgcacacgtg acaatcgata
     1381 catttctgca acacctgctg aacgccatcc aatggggcag cttcctggct ctgattgcct
     1441 gcggctacac gatggtctat ggcgttctgc tgatgatcaa cttcgcccac ggcgatatct
     1501 tcatggtggg cgcgtacctg gggtttttca tcggcacgtt cctgctcggt ggctttgccc
     1561 tgcgcctgcc cattgagctg ccgctctggg cggtcgtgct gctcacgctt gttatgacga
     1621 tgtcgctgac cgccctcgtg ggggtcacca tcgaacgcgt cgcttataga cctctgcgca
     1681 aaaaaggagc gcaacggctc tatctggtca tcaccgccct gatgttcggc ctgctgctgg
     1741 aatacggtaa tctggctctg ctcggcgcgc gcgaccgcca gttcccgcag ttgttcccca
     1801 agcaggtgta tacgttcggc ggcttgacca tcaccaatgt caagctgatg gtcatcctgc
     1861 tctcgatcct tacgatggtt acccttgcct tgctggtgca gcgaaccaaa tggggtatgg
     1921 ccatgcgcgc catctcctac gaccggctgg ccgtaccgct catgggcgtc tccaccgaca
     1981 cgatcatcat catgaccttc gtcgtcggct cggcgctggc ggcgctggcc ggggtcatgt
     2041 acggcacggc ctttcctatc ctgagctacg acatggggtt gctggtcggc tggcaggcgt
     2101 tcatcgccgc cgtcctcggc ggcatcggct cgattcgtgg ggcaatggtc ggcggctaca
     2161 tcctcggctt cacccaggta tttgtcgtag cctttctgcc gtccacttat cgtgacctca
     2221 tcacgttcac gctgttgctg gtcatcctgg tggtgcggcc gaccggcctt ttcggcgtgg
     2281 ctcgccggac caaggtatag attccatcca tgagacgcta cggactgacc atcctcgcca
     2341 tcgttgtgtt cctgctgacg gtgatcccgt cctggctggg gctatactcc ggctatatcc
     2401 agtttctcct ggccgtcatc ggcatcaata tcatcctgac cgtcagcctc aacctgatca
     2461 acggctatat gggtgaattc tccgtgggac atgcggcgtt catgggcgtc ggcgcttacg
     2521 tggcgtcgct cgtcacggtg tcgctgttcg gcgagaactc gctcctgggc gatgtgaacc
     2581 tgccgccggc gctctcgctg ctggtcttcc cggtggcgct gctcatcggg ggggtggccg
     2641 cggcgctctt cagcctgctc gtctccatcc cgtccttccg cacacggggc gactatctgg
     2701 ccatcatcac cctggcggtc aatttcatct tcaagagcct ggtggaaaac gtcgacgcca
     2761 tcggcgggcc gcgcggcttc atgggcatgg gcggcgtgtt gcgcgtgatg ggcggcgtgg
     2821 ccgatatacc ctggctgctt atctggacct ttctgtcgat ggtgttcacg atctacatca
     2881 tccgcaactt cgtcacctcg accatcggca agagcgtggt cgctatccgc gacgacgaga
     2941 tcgctgcaga ggtgatgacc gtcaacacgc ggcggctcaa gattattgct ttcatgctcg
     3001 gctgcggcct ggccggagtc gccggcgggc tgcacgccca tctcctgggt ttcatcaacc
     3061 catcgagcta cttcctgctc cagtccacgc tcatcctggt catggtctat ctgggcggga
     3121 tcggctcgat cagcgggtcg atcatcagcg ccatcgcctt cacggtgctg ctcgaactgc
     3181 tgcggccgct ggacctgttc cgctgggtgc tcacccccct gctgctcatc ggcctgatgc
     3241 tcttccggcc caccggcatc atgggcgacc gcgagct
//
LOCUS       DAOMQG010000368         3260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_11990_length_3260_cov_10.824961, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000368 DAOMQG010000000
VERSION     DAOMQG010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11990_length_3260_cov_10.824961"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLC06_14625"
     CDS             <1..207
                     /locus_tag="PLC06_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRO91729.1"
                     /translation="ELLPEAIALVRSLNKASTSLLQRRFRIGYTRAARLMDALEDQGI
                     IGPPTGTSKAREVYGGDGMMSDEG"
     gene            complement(355..807)
                     /gene="nusB"
                     /locus_tag="PLC06_14630"
     CDS             complement(355..807)
                     /gene="nusB"
                     /locus_tag="PLC06_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRO91730.1"
                     /translation="MKTRRRARRVTLETLYEYDIVSHDPAEVLEQRLEASPMERAGVE
                     FAQQLIHGVIAYQEQMDTLIARFAPEWPLDQMAVIDRNVLRIAIYEFLVTDETPVKVA
                     INEAVELAKVYGSDSAPRFINGVLGTLADHQEELRREILRGAAVAVTT"
     gene            complement(860..1219)
                     /locus_tag="PLC06_14635"
     CDS             complement(860..1219)
                     /locus_tag="PLC06_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRO91731.1"
                     /translation="MNEGQESIGRIEVAPEVLVTIARIAAQSIEGVQQMAQVPADYAR
                     LFQRGLRQDGVLLDLSENKVRFNIYAIMAPHVNLLEASRAIQTAVTESIDTMVGIQVD
                     AVNVFVEDVHYTKGEVV"
     gene            complement(1216..1962)
                     /gene="fabG"
                     /locus_tag="PLC06_14640"
     CDS             complement(1216..1962)
                     /gene="fabG"
                     /locus_tag="PLC06_14640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRO91732.1"
                     /translation="MLLEGKVAVVTGASRGIGRAIAEELAAEGAKVVVNYHSNAAAAE
                     EVVGGITARGGTAIALQADVSDFAAADALIKTAINTWGAIDILVNNAGTTRDTLLLSM
                     KEEEWDVVMTTNLKSIFNTCKAVTRPMVRRKQGGRIINISSVSGIVGQPGQTNYAASK
                     AGIIGFSKSLAKELGSRNITVNVVAPGFVPTDLTAIMPDDLVKQTLAFIPLGRWGEAQ
                     EIAHAVTFLASDKAAYITGAVIQVDGGIGT"
     gene            complement(2045..2947)
                     /gene="fabD"
                     /locus_tag="PLC06_14645"
     CDS             complement(2045..2947)
                     /gene="fabD"
                     /locus_tag="PLC06_14645"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRO91733.1"
                     /translation="MSTAFLFPGQGSQFVGMGRDLYDREPVARALFDEADALLGIPLS
                     QLCFEGPESALTDTAVQQPALFTTSLAAWAVLQQRGKANPTWVAGHSLGEFSALAAAE
                     AMSFADGLALVRRRGELMKLAGERSPGGMAAVLGLDAAPVAELCARAAAETGQYVGVA
                     NDNCPGQIVISGHNDALPRAIELITAAGARKVVRLPISIAAHTPLMATVADEFAAAID
                     ATPFSPTRAIVVGNVNAHPVQAPHEIRAELKAQLTSPVRWTESVRYLNEQGVDTYLEV
                     GPGDVLLGLVKRIDKEAQRVKFEI"
     gene            complement(3033..3221)
                     /gene="rpmF"
                     /locus_tag="PLC06_14650"
     CDS             complement(3033..3221)
                     /gene="rpmF"
                     /locus_tag="PLC06_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRO91734.1"
                     /translation="MGAVPKRKVSKARRDKRRTHDALSLYHLVPCSDCGEMKRSHRVC
                     PNCGKYNGRQIIPAASEE"
BASE COUNT          583 a          959 c         1036 g          682 t
ORIGIN      
        1 gagttgctgc cggaagccat cgccctggtg cgctctctca acaaggcctc gacctcgctg
       61 ctgcagcggc gcttccgcat tggctacacc cgcgccgccc ggctcatgga cgccctggaa
      121 gaccagggca tcatcggccc gcctacggga accagcaagg cgagagaggt gtatggggga
      181 gatggaatga tgagtgacga agggtgacgg acgacggacg acagacgaca agagagtgac
      241 gaattacgaa agaggcgcgc ttctcattcg taactcgtac tttggggagg gggctttcag
      301 gtcaaacaaa aacaggcctg ttggcctgtt ggatatcacg atgggtttta tttcttatgt
      361 cgttacggcc accgctgccc cccgcaaaat ctctcgccgc aactcttcct gatgatccgc
      421 cagcgtgccc agcacgccgt tgatgaagcg tggggcgctg tcgctgccgt agaccttggc
      481 cagttccacc gcctcgttga tagctacctt gaccggcgtc tcgtccgtca ccagaaactc
      541 gtagatggcg atgcgcagca cgttgcggtc gatgacggcc atctgatcca gcggccattc
      601 gggggcgaag cgggcgatga gcgtgtccat ctgctcctgg taagcgatga cgccgtggat
      661 gagctgctgg gcgaattcca ccccggcgcg ttccattgga ctcgcctcca gtcgctgctc
      721 cagcacctcg gccggatcgt gactgacgat gtcgtactcg tataaagttt ctaaggttac
      781 gcgacgggcg cgacgcctcg ttttcatact cttgctccta gaaaccgggt tttcggggga
      841 attcggtttc tagttgagat tagaccactt cgcccttggt gtaatggacg tcttccacga
      901 acacgttcac ggcgtctacc tggatgccga ccatggtgtc aatcgactcg gtgaccgctg
      961 tctgaatggc gcggctggcc tccagcaggt tgacgtgtgg ggccattata gcatagatat
     1021 tgaaacgcac cttgttttcc gacaaatcca gcaaaacgcc gtcctggcgc aagccacgct
     1081 ggaagagccg ggcatagtcc gccggaacct gggccatttg ctgcacacct tcgatgcttt
     1141 gtgcggcgat gcgcgcgatc gtcaccagta cttccggcgc aacttcgatg cggccgatgc
     1201 tctcttgtcc ttcgttcatg tgccgattcc tccatcgact tgaatcaccg ctccggtgat
     1261 ataggcggcc ttgtccgagg ccagaaaggt gacggcgtgg gcgatctcct gcgcctcacc
     1321 ccagcggcca aggggaataa acgccaaggt ctgcttcacc aggtcgtcgg gcatgatggc
     1381 cgtcaggtcg gtgggtacga agccgggggc cacgacgttg acggtgatat tgcgcgaacc
     1441 cagctcttta gccagagact tggagaaacc gatgatgccc gctttcgagg cggcgtagtt
     1501 ggtttggccg ggctggccga cgatgccgga gaccgacgag atgttgatga ttcggccacc
     1561 ctgtttgcgt cgcaccatcg gccgcgtgac ggccttgcag gtgttgaaga tgcttttcag
     1621 attggtggtc atcacgacat cccattcttc ttctttcatc gacagcaaca gggtgtcgcg
     1681 ggtggtgccg gcgttgttga cgaggatgtc gatcgcgccc caggtgttga tggcggtttt
     1741 gatgagggcg tcggcggcgg cgaaatcgct gacgtcggcc tgcagtgcga tggccgtgcc
     1801 gccccgtgcc gtgatgccgc cgaccacttc ctcggccgcg gcagcattgc tgtggtaatt
     1861 gacgaccact ttggctcctt cagcggccaa ctcctcggcg atggcgcggc cgatgccgcg
     1921 cgaggctccc gtgacgactg caactttgcc ttccagtaac attcgatctt cctgattgta
     1981 agtgtggcgc aagcgtttcg gcctgcgact cttttcctca acctcgatgg gctgagcacc
     2041 atcattatat ctcgaacttg actcgctgcg cttctttatc gattcgtttt acgagtccca
     2101 gcagcacgtc ccccggcccg acttcaagat aggtatcgac gccttgctcg ttcagatagc
     2161 gcaccgactc cgtccagcgg acgggggatg tcagttgggc cttcagttcg gcgcggatct
     2221 cgtgaggtgc ctggacggga tgggcattga cattgccgac gacgatggca cgggttgggg
     2281 agaagggcgt ggcgtcgatg gccgcggcga attcatcggc cacggtggcc atgagcggcg
     2341 tgtgggcggc gatggagatg ggcaggcgca cgaccttgcg cgcgcccgca gcggtgatca
     2401 gctcgatggc gcgcggcagg gcgtcgttgt ggccggagat gacgatctga ccggggcagt
     2461 tgtcgttggc aacgccgacg tattggcctg tttcggccgc ggcgcgggca caaagctcgg
     2521 ccacgggcgc ggcgtcaagc cccagcacgg cggccatgcc acccggactg cgctcgcccg
     2581 ccagcttcat caattcaccg cgccgacgca ccagcgccag cccgtcggca aagctcatgg
     2641 cttcggccgc ggccaacgct gaaaattcgc ccagcgagtg accagcgacc caggtcgggt
     2701 tcgccttgcc gcgctgctgc aagacggccc aggcggccag actggtggtg aacagtgccg
     2761 gctgctggac ggccgtgtcg gttagcgccg attccggccc ttcgaaacac aactgcgaga
     2821 gggggattcc gagcagcgcg tccgcttcgt cgaacaaggc gcgggcaacc ggctcgcgat
     2881 catagaggtc gcgccccatg ccgacgaatt gtgaaccttg tccggggaag aggaaggcgg
     2941 tggacattga tttgagcagg ggcgggcttt aagcccgccc ctgcaataat aaactcggac
     3001 aggggcgggt gatcgccctt acaatcattg aattattctt cgctggcggc ggggatgatc
     3061 tggcggccgt tgtatttgcc gcagttcggg cagacgcgat gcgagcgctt catctcgcca
     3121 cagtcggagc aggggacgag atgatacaga ctcagcgcgt cgtgggtgcg acgcttatcg
     3181 cggcgcgctt tagatacttt tcgcttggga actgcaccca tgatgttttt tcctcaattt
     3241 tagatttaac gccgcgggcg
//
LOCUS       DAOMQG010000369         3254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12021_length_3254_cov_10.170991, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000369 DAOMQG010000000
VERSION     DAOMQG010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12021_length_3254_cov_10.170991"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1232
                     /locus_tag="PLC06_14655"
     CDS             99..1232
                     /locus_tag="PLC06_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91735.1"
                     /translation="METAYGGRFSDFLRRHGTEGIMVLAANGKILSANRAAESLLGYG
                     ADQLTNLSITELWQGYSGTSRDAADWSRPHDVDLRRRNGDALPASVTVTALGEAADGE
                     RLISFVGRPDEERLNESLRHAQRMAGIGTLTAGVAHELTNPISIITAACANLLDELND
                     DTLSREDLAQTLALIEQSAFRCARIVDMLRNYAPNPGNDDGADDAAIAITDPSAILGD
                     ALTMVEQQFRKQARVTVTTEIQPELTTLFCDHHRIAQVLINLLLNARDAMQPAGGHIH
                     ARFWIPKLDREPALAGHVRAEMGRATSDLFAFSVSDTGPGVNPQDMSRLFEPFFTTKP
                     QGQGTGLGLFIAKGIVAQHGGCIFADNNFDGGATFTVVLPRKQ"
     gene            complement(1383..1964)
                     /gene="pdxT"
                     /locus_tag="PLC06_14660"
     CDS             complement(1383..1964)
                     /gene="pdxT"
                     /locus_tag="PLC06_14660"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRO91736.1"
                     /translation="MKIGVLALQGAFIEHVNALRKLGVEAVEVRLPEDLEGLDGLIIP
                     GGESTTIGKLADTFDLTEPLREFAESKPVWGTCAGMIFMAKEIGRDQPLLGLMDVVVE
                     RNAFGRQVDSFITPIEFKGIDGEPFPGVFIRGPRLVEAHGDAQVIARLADGTPVAARE
                     GKWLVTSFHPELTDDTRVHELFVKMVGESGGVQ"
     gene            complement(2064..>3254)
                     /gene="asnS"
                     /locus_tag="PLC06_14665"
     CDS             complement(2064..>3254)
                     /gene="asnS"
                     /locus_tag="PLC06_14665"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRO91737.1"
                     /translation="YRPEVGDELFERLVHLGQESSLVVTGTVRENAKAPGIPAGYEIG
                     IEEAVVLQDAVDYPIGPKDHGIDFLMDNRHLWLRSSQQWAILRVRAAIKRAIIDFFDD
                     RGFLNIDTPILTPSAAEGTSNLFEVDYFDEKAYLAQTGQLYNEANIMAFKKVYCFGPT
                     FRAEKSKTRRHLAEFWMVEPEIAYCDLDQLMDLEEAFVSYLVQTTLAKRAAELELLGR
                     DLTALRNITPPFPRISYDEAVERIQAIREATDDPELKEQLAIEWGDDFGSPHETVLTK
                     QFDKPVFVYGYPTAVKAFYMEPWPGRPEVCKSVDLLAPEGYGEIIGGSERMSDPAVLL
                     EAIRRHELPESNYSWYIDLRRYGSVPHSGFGLGLERTVAWICGIDHIRQTIPFPRTLN
                     RLTP"
BASE COUNT          578 a         1006 c         1045 g          625 t
ORIGIN      
        1 ttcttcacgc tgaatttacg acaggcgcac ggacggccgc ccgtcgctcg cgtatccttt
       61 gggcgcaagg tggtattcgc ggctggaggg ccttataagt ggaaactgca tatggtggcc
      121 ggttctccga cttcctgcgc cgtcacggga cggaaggcat catggttctg gccgcgaacg
      181 ggaagatact gtcagcgaat cgggcggccg aatcgctatt agggtatggg gctgatcaat
      241 tgacgaattt gtcgatcact gaattgtggc aaggatatag cggaacatcc cgtgacgcgg
      301 ccgattggtc gcgccctcat gatgtcgact tgaggcggcg aaacggcgac gcgcttccgg
      361 cgtcggtgac ggttacggcg cttggggagg cggccgacgg cgaacgcctc atctcgtttg
      421 tcggccggcc ggacgaggag cggctcaacg agtcactgcg ccacgcgcaa cgaatggccg
      481 gcatcggcac gctgacggcc ggcgtcgccc acgagctgac caacccgatc agcatcatca
      541 ccgctgcgtg cgccaacctt ctggatgaac taaatgacga cacgctcagc cgcgaagacc
      601 tggcgcagac gctcgcgcta atcgagcaga gcgcgttccg ctgtgcgcgc atcgtcgata
      661 tgctgcgcaa ctatgcgccc aatccgggga atgatgatgg cgcggatgat gccgccatcg
      721 ccatcaccga cccgtcagcc atcctgggag acgccttgac gatggtggag cagcaattcc
      781 gcaagcaggc gcgggtgacg gtgacgacgg agattcagcc ggagttgacg accctcttct
      841 gtgaccacca tcgcatcgct caggtgctga tcaacctgct gctcaacgcc cgcgacgcga
      901 tgcagccggc cggcggccac atccacgcgc gcttctggat ccccaaactc gatcgcgagc
      961 cggcgctagc cgggcatgtg cgcgccgaga tgggccgggc cacgtccgat ctattcgctt
     1021 tctcggtgtc cgacaccggg ccaggtgtca acccacagga catgagccgt ttgttcgagc
     1081 cgttcttcac cacgaagccc cagggccagg gcacggggtt gggcttgttc atcgccaagg
     1141 ggatcgtcgc gcagcatggc ggctgcatct tcgccgacaa caacttcgat ggcggggcga
     1201 cgttcaccgt cgtgctgccg cgtaagcagt aggtcagtgg gtcagtgacc agtaggtcag
     1261 tggatcagta ggtcagtaga tcagtccgcg aaactgactc gctgacctac tgacctactg
     1321 aatactgacc tactgaatac tgacctactg aatactgacc tactgaccta ctgaatactg
     1381 acctactgaa ccccgccgct ctcccctacc atcttcacaa acagctcgtg gacgcgcgtg
     1441 tcatcggtca gctcggggtg gaatgacgtg accagccatt tgccttcgcg ggcggccacg
     1501 ggcgtgccgt cggccaggcg ggcgatgact tgcgcgtcgc cgtgggcttc aacaaggcgc
     1561 gggccgcgga tgaagacgcc ggggaatggc tcgccgtcga tccccttgaa ctcgatgggt
     1621 gtgatgaagc tgtcgacctg gcggccgaaa gcattgcgct cgacgaccac atccatcaag
     1681 ccaagcagcg gctgatcgcg gccaatctcc ttggccatga agatcatccc cgcgcacgtg
     1741 ccccagaccg gtttgctttc ggcgaattcg cgcagcggtt cggtcaggtc gaaggtgtcg
     1801 gccaatttgc cgatggtcgt gctctcgccg ccgggaatga tgaggccgtc cagcccttcc
     1861 aggtcttcgg gcagccgcac ctcgaccgcc tcaacgccca gtttccgcag agcgttgaca
     1921 tgctcgatga aggcgccttg cagcgccaga acgccgattt tcatttgatt tttcctgtgg
     1981 cgcaagccgc cggcttgctt cttgttccac tctcaacctg gcaagttgag ctacaactgc
     2041 caggttgagc tatagcattg ttcttacggc gtcagccggt tgagcgtgcg cgggaagggg
     2101 atggtctggc ggatgtggtc aatgccgcag atccaggcca ccgtgcgctc cagccccagg
     2161 ccgaagccgc tgtggggcac gctgccgtag cgccgcaggt cgatgtacca gctgtagttg
     2221 ctctccggca gttcgtggcg gcggatggcc tccagcagca cggccgggtc gctcatgcgc
     2281 tcgctgccgc cgatgatctc gccatagcct tcgggggcca gcaggtcgac gctcttgcac
     2341 acttccggcc ggccgggcca cggctccatg tagaacgcct tcacggccgt cgggtagccg
     2401 tagacgaaga ccggcttgtc gaactgcttc gtcagcaccg tctcgtgcgg cgagccgaag
     2461 tcgtcgcccc actcgatggc cagttgttcc ttcagttcgg ggtcgtcggt ggcctcgcgg
     2521 atggcctgaa tgcgctcgac cgcctcgtcg tagctgatgc gcgggaaggg cggggtgata
     2581 ttgcgcaagg ccgtcaggtc gcggccgagc agttccagtt cggccgctcg cttggccagc
     2641 gtggtctgca cgagatagct gacgaacgct tcttcaagat ccatcaactg atccagatcg
     2701 caataggcga tctccggctc caccatccag aactcggcca gatggcggcg ggtcttgctc
     2761 ttctcggcgc ggaaggtggg gccgaagcag tagaccttct tgaaggccat gatgttggcc
     2821 tcattataga gctggccggt ctgggccaga taggctttct cgtcgaagta gtcgacctcg
     2881 aacaggttgc tggtgccctc ggccgcgctg ggggtcagga tgggcgtgtc gatattgagg
     2941 aagccgcgat cgtcgaagaa gtcgatgatc gcccgcttga tcgccgcccg cacgcgcagg
     3001 atggcccatt gctgcgagga gcgcagccac agatggcggt tgtccatcag gaagtcgatg
     3061 ccgtggtcct tggggccgat ggggtagtcg acggcgtcct gcaggacgac ggcctcctcg
     3121 atgccaatct cgtagccagc gggaatgccg ggggccttgg cgttctcgcg cacggtgccg
     3181 gtgacgacga gcgacgactc ctggcccagg tggacgagcc gctcgaacaa ttcgtcgccg
     3241 acttccggcc ggta
//
LOCUS       DAOMQG010000370         3226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12160_length_3226_cov_9.073794, whole genome shotgun sequence.
ACCESSION   DAOMQG010000370 DAOMQG010000000
VERSION     DAOMQG010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12160_length_3226_cov_9.073794"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..986
                     /locus_tag="PLC06_14670"
     CDS             327..986
                     /locus_tag="PLC06_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRO91738.1"
                     /translation="MITLEQVRADEEVGVLIDYANQYLRALGYTEHSTRHATRVAETA
                     RNILLEAGHPERVAELAAIAGYLHDIGNLIHREGHAQSGSLIALRVLTRLGMPPDERA
                     VIANAIGNHEEERGYATSPVSAALNIADKADVHRSRVQAESFQDFDIHDRVNYATTQS
                     QVVVDAPNQIINLELTIDTSFAQVIEYFEIFLDRMVMVKQAAEFLGLHFHLIINGTTF
                     S"
     gene            complement(1040..2593)
                     /gene="hutH"
                     /locus_tag="PLC06_14675"
     CDS             complement(1040..2593)
                     /gene="hutH"
                     /locus_tag="PLC06_14675"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRO91739.1"
                     /translation="MDPLLISGENLHIDDVVAVAQGRKVALDPAAVPLMERSRAAVDR
                     LVAEGRAVYGITTGFGRFKDRLISPDEVRQLQLNLVRSHAVGVGPDLPDDAVRAIMLA
                     RANTLALGHSGARPAIVRLLVDMLNAGITPRIPAQGSLGASGDLAPLAHLALVVIGEG
                     EAKMDGRLMSGGAALAAAGLQPVELEAKEGLALLNGTALMVGMGALLVRRSINLALTA
                     DVVACLSLEALHGTDRAYDPRVHALRPHPRQIKCADFLRNVLAGSAFLRRDDPNNVQD
                     AYTLRCVPQVHGAVRDSIAYAQWVIDIELNTVNDNPIIFVDDAGEIDVVSAGNFHGEP
                     VALAMDYAALGLTELGNMSERRCARLVDADSNGGVLPMFLTANGGLQSGLMMAQYTAA
                     ALASENKVLAHPSSADTIPTSANVEDHVSMGAAAVRHAGQILEHVETIVGIELLLAAQ
                     GIEFRSREMGLTPADLGRGTAEAYQLIRERVPFLESDVMLAPLIEKARELVSSGAIKR
                     AAEVKLGLG"
     gene            2654..>3226
                     /gene="holB"
                     /locus_tag="PLC06_14680"
     CDS             2654..>3226
                     /gene="holB"
                     /locus_tag="PLC06_14680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRO91740.1"
                     /translation="MNTLAPGWRTIVGHDWAVRLIANAILHDRVGHAYLITGPEQIGK
                     MTLARTFAQALNCTAESGLRPCGACRACQLIQADKHPDVRVILPEVSERGAMSIKIEQ
                     MRRLQQDLSLSTYEANYKVAILKRFDTANLNAANAFLKTLEEPPSNVVLILTATDSDT
                     LLPTINSRCRTIGLRPMPADLIEETLMTRYH"
BASE COUNT          665 a         1040 c          925 g          596 t
ORIGIN      
        1 gttatcctgc ctttgtaaat tcaagtggtg atcacgacga aacatgaagt cacgctcaca
       61 gagcgttcgg gggtcttaat tcgataagtc tgttcctcct cggtgttgaa ataccagcca
      121 aaggccacgg ttaagtgcct gggaactgag ttgaatcaag tatgaaatgt tgtctgctca
      181 gaacaacata tttacagtat gcagagaatt tcaaaccaaa cgtgacaata tcatcataat
      241 agggatgcag gccattgtca agcgggcaat attactcatt ttcagttccg gggcaaaagg
      301 gtcccatctt caaaggaggt atctgaatga tcacattgga acaggtgcgc gcagatgaag
      361 aagtcggcgt gctgattgac tacgcaaacc aatatttgcg cgctttgggc tataccgaac
      421 acagcactcg ccacgccact cgtgtcgccg aaaccgcccg caacattctg ctggaggccg
      481 gccaccccga acgcgtcgcc gagctggcgg ccatcgccgg ctatctccac gacatcggca
      541 acctgattca tcgtgaaggt catgcgcaga gcggcagcct catcgccctg cgcgtgctca
      601 cgcgcctggg catgccaccg gacgagcgcg ccgtcatcgc caacgccatc ggcaaccacg
      661 aagaggaacg tggttatgcc acatcacccg tctcggccgc gctcaacatc gccgacaagg
      721 ccgatgtcca ccgcagccgc gttcaggctg aatccttcca ggatttcgat atccacgacc
      781 gcgttaatta cgccaccaca caaagtcagg ttgtcgtcga tgcgcccaac cagatcatca
      841 acctggaact gaccatcgac acaagcttcg cgcaggtgat cgaatatttc gagattttcc
      901 tggatcggat ggttatggtg aagcaggcgg ccgaatttct gggcctccac ttccatctca
      961 tcatcaacgg cactacgttc tcctgacgct gtctcgccgg agaaaagccg ggttgaaggg
     1021 cgacccggca gcgcgcggat catcccaggc ccagcttcac ttccgcggca cgtttgatcg
     1081 ccccgctgct caccagttcg cgcgcctttt cgatgagtgg agccagcatc acgtcggatt
     1141 ccaggaacgg gacgcgctcg cgaatcagtt gataggcctc ggccgttccc cggcccagat
     1201 cggcgggcgt cagccccatc tcacgcgagc gaaattcaat cccctgggcc gccagcagca
     1261 gctcgatgcc gacaatagtc tcgacgtgtt ccaggatttg gcccgcgtgc ctcacggcgg
     1321 ccgcgcccat actcacgtga tcttcgacgt tggcactggt ggggatggtg tcggcgctcg
     1381 acgggtgggc cagcaccttg ttttccgaag ccagcgccgc ggccgtatac tgggccatca
     1441 tcaacccgct ctgcaaacca ccattggccg tcaggaacat gggcagcacg ccgccgttgc
     1501 tgtcggcatc caccagtcgg gcacagcgcc gctcgctcat gttgcccagt tccgtcaacc
     1561 ccaacgcggc gtagtccatc gccagtgcca ccggttcgcc gtggaagttc ccggccgaga
     1621 cgacgtcgat ttcacccgcg tcgtcgacaa agatgatcgg gttgtcgttg acggtgttga
     1681 gttcgatatc gatcacccac tgcgcatacg cgatggagtc gcgcaccgcc ccgtggacct
     1741 gcggcacaca gcgcaacgtg taggcatcct gcacattgtt ggggtcgtca cgtcgcagaa
     1801 aagcgctgcc cgccaatacg ttgcgcagaa agtcggcgca tttgatctgg cgcggatgcg
     1861 ggcgcagggc atggacgcgc ggatcatagg cccgatccgt cccatgcagc gcctccagcg
     1921 acagacaggc gacgacgtcg gccgtcaacg ccaggttgat gctccggcgc acaagcagcg
     1981 cgcccatgcc gaccatcaag gccgtgccgt tgagcagcgc cagcccttct ttggcctcca
     2041 gttcaacggg ctgcagcccc gcggcggcca gcgccgcccc gcccgacatc aggcggccgt
     2101 ccattttggc ttccccttca ccgatgacaa ccagcgccaa atgggccagc ggggccagat
     2161 cgccgctcgc gcccagagag ccttgagccg ggatgcgcgg ggtgatgccg gcgttgagca
     2221 tatcgaccag caggcgcacg atagccgggc gcgcgccgga atggccaagg gccagcgtgt
     2281 tggcgcgagc cagcataatc gcccggacgg catcatcggg caagtccggc cccacgccca
     2341 cggcgtgact gcgaaccagg ttcaactgga gctgccgcac ctcatcgggc gagatcagcc
     2401 gatccttgaa gcggccgaag ccagtcgtaa tgccgtagac agccctgccc tcggccacta
     2461 atcggtcaac cgcggcgcgt gatcgctcca tcagcggcac ggccgcgggg tcgagcgcca
     2521 cctttcgccc ctgagcaacg gcgacgacat catcaatgtg taaattttca ccggagatta
     2581 gcaatggatc catggcgcga ttataacaga gcgatcgttt tcgtctttta ttgggccggg
     2641 gtacaatgtc ggcatgaata ccctcgcgcc gggctggcga acgatcgtcg gccatgactg
     2701 ggctgtgcgg ctgatagcca acgccatcct gcatgatcgg gtcggccatg cctatctgat
     2761 caccgggccg gagcagatcg gcaagatgac cctggcgcga acattcgccc aggcgctgaa
     2821 ttgcaccgcc gaaagcggcc tgcgtccctg tggcgcctgt cgcgcttgcc aattgattca
     2881 ggccgacaag caccctgacg tgcgcgtcat cctgcccgag gtgagcgagc ggggcgcgat
     2941 gtcgatcaag atcgagcaaa tgcggcggct ccagcaagac ctgagcctct ccacttatga
     3001 agcgaattat aaggtggcca tcctgaaacg cttcgatacg gccaacctga acgcggccaa
     3061 tgcctttctg aagacgctcg aggagcctcc cagtaacgtg gtcctcatcc tgaccgctac
     3121 cgacagcgac accctgctgc cgacgatcaa ctctcgctgc cggaccatcg gcttgcgacc
     3181 gatgccggcg gacctgatcg aagagacgct gatgacccgc tatcat
//
LOCUS       DAOMQG010000371         3219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12185_length_3219_cov_8.767067, whole genome shotgun sequence.
ACCESSION   DAOMQG010000371 DAOMQG010000000
VERSION     DAOMQG010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12185_length_3219_cov_8.767067"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1390)
                     /locus_tag="PLC06_14685"
     CDS             complement(167..1390)
                     /locus_tag="PLC06_14685"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRO91741.1"
                     /translation="MTHSQPFVLGVNYWPRRKAMYWWSDFDAAEVREEFALIRDLGMQ
                     LVRVFLLWDDWQPTPDTVNAEALENLGTVLNIAHELDLKLDITFFTGHMSGPNWSPGW
                     LLETGAPLPPRVMQLVSGGRIVDCGYRNPYTDPIALAAAGLLLRTVVGRYHDHPAVGF
                     WNLGNEPDLFAWPPDPATGREWVRRMTGLIREIDPIHPVTCGLHMDSIVGDNNLRVHD
                     VFGEVDFAVMHSYPMYTPWARGPLDPDFVPFSCALTAALSGKPVLMEEFGGCTNTPGL
                     PSETWEWTAYGKPRTQFMASEEALADYLESVLPRLIDVGATGAVLWCFADYAPELWDR
                     PPCKESVHERFFGLVRPDGSLKPHAEVIRRFAAANPIVRPAIHTVDLDITPETYYDDP
                     AGHAVRLYEQFLANR"
     gene            complement(1413..2135)
                     /locus_tag="PLC06_14690"
     CDS             complement(1413..2135)
                     /locus_tag="PLC06_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRO91742.1"
                     /translation="MSGEYIPKTAMMIFAHPDDIEFGVAGTAAKWARAGSRVIYVVIT
                     DGNVGSHEAGMTAEKLAEIRRAEQTAAADVAGAECVFLGYHDGFLQPTLELRKDLVRL
                     IRQYKPDAVVCMDPTNIFPSDDYINHPDHRAAGTAALDAVFPAAEMEMLYPDLAAEGL
                     NGHKVNYVYVMFSDKANYYIDITDTIDLKIEALKKHVSQLETGEVADMIRQWAAEAGK
                     KVGFAFAEGYYRIALHAPETAG"
     gene            complement(2236..3090)
                     /locus_tag="PLC06_14695"
     CDS             complement(2236..3090)
                     /locus_tag="PLC06_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HRO91743.1"
                     /translation="MSSEIPKPTIVAFVADLMFTMRIARVIDHIGYEVVWIESAGQLV
                     SQSERVAPELPGEQLQGHEGRLFEEIVRIQPALMLFDLNNAGIPWREWIPLLKSAAAT
                     RRIPVMCFGPHEDVDAMTTARSAGADVVLGRSRFTSDMPALLQRYARIPDRHALAGAC
                     AEPLSDLAREGIERFNRGEYYPAHDALEEAWVVDQGPGRDLYRGILQVGIAYYQIEKG
                     NYRGAAKMLLRVRQWLAPLPPVCRSVDVAALRDDVERVYSALTSLGPDRVEDFDRQLL
                     GPVITHPL"
BASE COUNT          587 a          950 c         1037 g          645 t
ORIGIN      
        1 gcgaatgaat gcgaatgaat gcgaatgaat gcgaatgaat gcgaatgaat gcgaatgaac
       61 gcgaatgaac gcgaatgaat gcgaatgaac atgttaaatc agtgaaatcc gtgtcgtccg
      121 cgttctgttc gctccttcat cttttcatgc gattgccctg ggcgcgctaa cgattggcca
      181 ggaactgttc gtagagcctg acggcgtgcc cggccggatc gtcatagtac gtctcgggtg
      241 taatgtccag atccaccgtg tgaatcgccg ggcgaacgat ggggttggcg gccgcaaagc
      301 gcctgatgac ctcggcgtga ggcttgagcg agccatcggg ccggacgagg ccgaagaacc
      361 gttcgtgaac cgactccttg catggcggcc gatcccataa ctccggcgcg taatcggcga
      421 agcaccacag cacggcgccg gttgcgccga cgtcgatcag ccgcggcagg acgctctcca
      481 gatagtcggc cagcgcttct tccgaggcca tgaactgggt tcgcggcttg ccgtaagccg
      541 tccattccca cgtttcggaa ggaagtcccg gcgtgttcgt gcagccgccg aactcttcca
      601 tgagcaccgg cttgccggac agggcggcgg tcagggcaca gctgaagggg acgaagtcgg
      661 gatcgagggg gccgcgcgcc cacggcgtgt acatggggta gctgtgcatc accgcgaagt
      721 cgacctcacc gaagacgtca tggacgcgca gattgttgtc gccgacgatg ctgtccatgt
      781 gcaggccgca ggtgaccgga tgtatggggt caatctcgcg gatgaggccg gtcatgcgcc
      841 gcacccattc ccgacccgtc gccggatcgg gcggccaggc gaacagatcc ggttcgttgc
      901 ccagattcca gaagccgacc gccggatggt cgtgatagcg gccgacgacc gtgcggagca
      961 gcaacccggc cgccgccagc gcgatcgggt cggtgtaggg gttgcgatag ccgcaatcga
     1021 ctatccggcc gccgctgacc agttgcatca cgcggggcgg cagcggcgcg ccggtctcca
     1081 gcagccagcc gggcgaccag ttcggcccgc tcatgtggcc ggtgaagaag gtgatgtcca
     1141 gctttagatc caattcgtgg gcgatgttca ggaccgttcc gagattttcg agcgcctccg
     1201 cgttgactgt gtcgggcgtg ggctgccagt cgtcccataa cagaaagacg cgcacgagct
     1261 gcatgccgag gtcgcgaatg agggcgaact cctcgcgcac ctcggccgcg tcgaaatccg
     1321 accaccaata catcgccttg cgtcgcggcc agtagttgac tcccaggaca aagggttgcg
     1381 agtgggtcat ggcgttggct cctctgtcgt ggttatccgg ctgtttccgg ggcgtgcaat
     1441 gcgatgcgat agtacccttc ggcaaaggcg aagccgacct tcttgccggc ttcggccgcc
     1501 cattggcgaa tcatatcggc cacttccccg gtttccagtt ggctgacatg ttttttcagg
     1561 gcctcgattt tcagatcgat ggtgtcggtg atgtcgatgt agtagttggc cttgtcgctg
     1621 aacatgacat agacgtaatt gactttgtgt ccattcagtc cttccgctgc cagatcgggg
     1681 tagagcatct ccatttcggc cgccgggaag acggcatcga gcgcggccgt gcccgcggcg
     1741 cgatgatcag gatggttgat gtagtcgtcg ctggggaaga tgttggtggg gtccatgcag
     1801 acgacggcgt cgggcttgta ttgccggatc agtcggacca ggtccttgcg cagttccagc
     1861 gtgggctgca gaaagccgtc atggtagccg aggaagacgc actccgcgcc ggcaacgtcg
     1921 gccgcggccg tttgctcggc ccggcgaatc tcggccagct tttcggcggt catgcccgct
     1981 tcatggctgc ccacgttgcc gtcggtgatg accacgtaga tgacccggct gccggcgcgc
     2041 gcccacttgg cggccgtgcc ggccacgccg aattcgatgt cgtcggggtg ggcgaatatc
     2101 atcatcgccg ttttgggaat atattctccg ctcatttgtt gcctctcttt tgtcgatcaa
     2161 tcaaccctga ttgatatcga cgtgtgatat tgtgccaaca ggcgcgctcg atcaagaata
     2221 ttcgcgcttt cggatttaca gggggtgggt gatgacgggg cctaacagct gccggtcgaa
     2281 atcctcaacc ctgtccggcc ccagggacgt gagcgccgag tagacccgct ccacgtcatc
     2341 ccgcagggcg gctacgtcca cgctccggca aaccgggggc agcggggcca gccactgccg
     2401 cacgcgcagc agcatcttgg ccgcgccacg gtaattgcct ttctcaatct ggtagtaggc
     2461 gatgccgact tgcaggatac cccggtatag atcgcggcca ggcccctgat cgacaaccca
     2521 cgcctcttcc agtgcgtcgt gggccgggta gtactcaccg cgattgaacc gttcgatgcc
     2581 ttcccgcgcc aaatcggaca gcggttcggc gcaggcgccg gccagcgcgt gccggtcggg
     2641 tatacgcgcg tagcgctgga gcagggcggg catatcggac gtgaagcgcg agcgacccag
     2701 gacgacgtcc gcgcccgcgc tcctggcggt cgtcatggcg tccacgtcct cgtgtgggcc
     2761 gaagcacatc acggggattc ggcgggtggc ggccgcggat ttcagcaggg ggatccattc
     2821 gcgccagggg atgccggcgt tgttcagatc gaacagcatc aatgccggct gaatccgaac
     2881 gatctcctcg aacagacgcc cctcgtggcc ctggagttgc tcgccgggca gttccggcgc
     2941 gactcgctcg ctctgtgata cgagttggcc ggccgattcg atccagacga cttcgtagcc
     3001 gatatggtcg atcaccctgg cgattcgcat cgtgaacatg agatcggcga caaaagcgac
     3061 gatcgtgggc ttgggtattt cagacgacat agataaagac gatggggccg acaggacttt
     3121 ctctgcctga tgaaccggtt tgaacttaac tgtcttgcgt cacgcggtga tgatcaacag
     3181 gtgatgtccg tttgatattt tcctaactat caggattat
//
LOCUS       DAOMQG010000372         3192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12312_length_3192_cov_8.254383, whole genome shotgun sequence.
ACCESSION   DAOMQG010000372 DAOMQG010000000
VERSION     DAOMQG010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12312_length_3192_cov_8.254383"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLC06_14700"
     CDS             <1..1080
                     /locus_tag="PLC06_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRO91744.1"
                     /translation="NDLDMVEEMRLASFIAKVSTSDPTAMPARQALELATIGGARAIH
                     MSDVTGSLEPGKRADIAVLDMTGIHNQPHFHNHPDAVYSRIVYSTKSTDVSHVMCNGR
                     WLMRERQLLTVDEDAARRDAIEVAKAIDAFVIRRESSPYNKLVLLSGVQRQESFEVQV
                     KVPLENEAAVEALLANEAITVSRYAHYRQYDHYFLFDGGDPDADRLRYREDEFINDDG
                     EVTQVRARLTLTGAGGREEFPNAVMLSRSRWLAAADRSLRFYQEYFAPARELRVHKDR
                     RRWHVLYKATDFAINLDQVLEPKLPGYFLEIKARTWSRSDAERKAGLIAELLTQCGLE
                     VDWAERREYTDIAISANKARDAAPA"
     gene            1094..2947
                     /locus_tag="PLC06_14705"
     CDS             1094..2947
                     /locus_tag="PLC06_14705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HRO91745.1"
                     /translation="MPLLPGEILHNRYRVAGLLARGPHGAVYRGWDATDRRDVAIKEY
                     LDPSVEIQKRFRAEARRLAGLSHPQLPRVLDHFALDNGQYLVSEFIDGVDLQSLIDQY
                     GPLPSDLVAPWLQSATAPLAYLHKQNRLHLDIKPANIRLTPDGQVFLVDSGLPGLGTR
                     PHSPGYGAPEQQAQGDVGPTADVYSLGATLYTALTGKVPPNALSRETGLSDLLPAREA
                     NPDVEPYLSIVAGRAMSLRPDTRYDTAEEFARALERPAGRPAPAVSPMRRAGPEPVPA
                     AAPAPRLSQGRRRRLDRRSLLGLAIIGLLIVVIAAFLVLTNLEQPEQVSGPAATSTLQ
                     SAVIAALTQLAPTPTDTPEPTIPPTPSPQPLLTETGSRMIFIPGGTFEIGEDSSEEND
                     EKPARLVTLDSFYIDETEVTNAAYAQCVAAEACPRPARAGATYYLTYFGDPNFDDYPV
                     INVSWYSADAFCAWRGARLPSEAEWEFAASYDAAERRERRYPWGDTFDGTILNFCDVN
                     CQRDDRGFEWDDGFRDTAPVGSYPDGRSPMGVYDMLGNVAEWVGDWYDFRAYRDIADT
                     NPRGPVDGEFKVMRGGSWFTPPGNLGTFIRDNLDPNAAQATVGFRCAMLPP"
BASE COUNT          545 a         1133 c         1019 g          495 t
ORIGIN      
        1 aacgacctgg atatggtcga ggaaatgcgc ctggcgtcct tcatcgccaa ggtctccacg
       61 tccgatccga cggcgatgcc cgcccggcaa gcccttgaac tggccaccat cggcggggcg
      121 cgcgccatcc acatgagcga cgtgaccggc agcctggagc cgggcaagcg ggccgacatc
      181 gcggtgctgg acatgaccgg catccacaac cagccccact ttcacaacca ccccgatgcc
      241 gtctactcgc gcatcgtcta ctccaccaag agcaccgacg tgagccacgt catgtgcaac
      301 ggccgctggc tcatgcgcga gcgccaactg ctgacggtgg acgaagacgc cgctcgccgc
      361 gacgccatcg aggtggccaa ggcgattgac gctttcgtca tccggcgcga gtccagcccg
      421 tataacaagc tggtgctgct gtcgggcgtc cagcgccagg agagcttcga ggtgcaggtg
      481 aaggtgccgc tggaaaacga ggccgccgtc gaggcactgc tggccaatga agccatcacc
      541 gtttcccgct atgcccacta ccgccagtat gaccactatt tcctgttcga cgggggcgat
      601 cccgacgccg accggctgcg ctatcgcgag gatgagttca tcaacgacga cggcgaggtg
      661 acccaggtgc gggcgcgact gacgctcacc ggcgcgggcg gccgcgagga gttccccaac
      721 gcggtgatgc tctcccgctc acgctggctg gccgcggccg accggagcct gcgcttctat
      781 caggaatatt tcgctccggc ccgcgagctg cgcgtccaca aggatcgccg gcgctggcac
      841 gtgctgtata aggccaccga cttcgccatt aacctcgatc aggtgctgga gccgaagctg
      901 ccgggctact tcctggagat caaggcccgc acctggtcgc gcagcgacgc cgagcgcaag
      961 gcggggctga tcgccgaact gctgacccaa tgcggccttg aggtcgattg ggccgagcgg
     1021 cgcgaataca ccgatatcgc tatcagcgcc aacaaggcca gggacgcggc accggcctga
     1081 gctgacccag cctatgcctt tgctgcccgg cgaaatactc cacaatcgct atcgcgttgc
     1141 cggcctgttg gcgcgcggcc cgcatggggc cgtctatcgc ggttgggacg cgaccgaccg
     1201 gcgcgacgtg gcgattaagg agtatctcga cccgtcggtg gagatacaga agcggttccg
     1261 ggccgaggcg cggcggctgg cggggctgag ccatccgcaa ctgccgcgtg tgctggatca
     1321 cttcgcgctc gacaacggcc aatacctcgt cagcgaattc atcgacggcg tcgatctgca
     1381 aagcctgatc gatcagtatg gcccgctgcc gtcggacctc gtcgcgccgt ggctgcaaag
     1441 cgcgaccgcg ccgctggcct atctccacaa gcaaaaccgg ctgcatctgg acattaaacc
     1501 ggccaacatc cgcctgacgc ccgacggcca ggtgttcctc gtcgatagtg gcctgccggg
     1561 gttgggcacg cggccgcatt cgccgggcta tggcgcgccg gaacagcagg cgcaggggga
     1621 tgtcggcccg acggccgacg tctacagcct gggggcgacg ctctacaccg ctctgaccgg
     1681 caaggttccg cccaacgcgc tcagtcgcga gacgggcctc agcgacctgc tgccggcccg
     1741 cgaagccaac cccgacgtgg agccatatct ctccatcgtg gccgggcgag ccatgtcgct
     1801 gcggccggac acgcgctatg acacggccga ggagttcgcg cgggcgctgg aacgtcccgc
     1861 gggccgccct gccccggcgg tcagccccat gcgccgcgcc gggccggagc ctgtgcccgc
     1921 cgctgccccc gcgccgcgcc tgtctcaggg ccgccgccgt cgtctcgacc ggcgttcgct
     1981 gctggggttg gcgatcattg gcctgttgat cgtcgtgatc gccgcctttc tggttctgac
     2041 caacctggag caaccggagc aggtcagcgg cccggcagcc accagtacgc tgcaatcggc
     2101 ggtcatcgcc gcgctgaccc agctcgcgcc cacgcccacc gacacacccg aaccgaccat
     2161 tccgcccacg ccgtccccgc agccgctcct caccgagacc ggctcgcgga tgatcttcat
     2221 ccccggcggg acgttcgaga tcggcgaaga cagcagcgag gaaaacgacg agaagcccgc
     2281 gcgactggtg acgctcgact cgttctacat cgacgagacc gaggtgacca acgccgccta
     2341 cgcccaatgc gtcgccgccg aggcctgccc gcggcccgcc cgcgccggag cgacctacta
     2401 tctgacctac ttcggcgacc cgaacttcga cgactacccg gtcatcaatg taagctggta
     2461 cagcgccgac gcgttctgtg cctggcgcgg ggcgcggttg cccagcgagg ccgagtggga
     2521 gttcgccgcc tcttatgacg cggccgagcg ccgcgagcgc cgctatccgt ggggcgacac
     2581 gttcgacggc acgatcctca atttttgcga cgtgaattgc cagcgcgacg accgcggctt
     2641 cgaatgggac gacggcttcc gcgataccgc gccggtgggc agctaccccg acggccgttc
     2701 gcccatgggg gtctatgaca tgctcggcaa cgtggccgag tgggtgggcg actggtacga
     2761 cttccgcgcc tatcgcgaca tcgccgacac caacccgcgc ggcccggtgg atggcgagtt
     2821 caaggtgatg cgcggcggct cgtggttcac cccgccgggc aatctgggaa cgttcattcg
     2881 cgacaatctg gaccccaacg ccgcgcaggc cacggtcggg tttcgctgcg cgatgttgcc
     2941 gccgtagggg ggacgaccgc tgacgacgga cgacagaccg cagacgacag accgcagacc
     3001 gccgaccgca gacgacagac cgcagacggc cggctgctgt gggaaggacc gctcccctct
     3061 tgcggcggtc gtcggtcgtc cgtcgtccgt cgtccgtcat ctatagccct ctccccacct
     3121 ttatgctaaa cataccccat gaaccgtatc acctctcccc aggatatcga ccggttggaa
     3181 gacctcatcg gc
//
LOCUS       DAOMQG010000373         3186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12336_length_3186_cov_8.646119, whole genome shotgun sequence.
ACCESSION   DAOMQG010000373 DAOMQG010000000
VERSION     DAOMQG010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12336_length_3186_cov_8.646119"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="PLC06_14710"
     CDS             <1..1282
                     /locus_tag="PLC06_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91746.1"
                     /translation="EAGVYGHEFSGSTRDYYQLLKVASQVAKANDPEAVIHLAGWSYW
                     HDPTWLAQFLNVATSDPEGPANGYFFDAITLHIYFRVETVEELVQETWEIQRRYGLKK
                     PIWINETNASPNLDPLWPVERPNFQVNLEQQAWYIVQAHALGFGANASSIGVYKLLDI
                     LLPAGGESFGILRPDGSRRPAFDAYKTTIRYLRDFTFPVGRQQEKDFYSFTFKRPEGF
                     TRVLWARNSNDQILKVPAITGEARLISALGDTETTLRAYKGFYRIRLRGALCNPACDI
                     GGAPVFLVEETSADVPVESVPLISPRDSILATPTPTAVVTATIMPDGTVTAEASPEPT
                     QTQTATPHPSETPTIEPTATTAPTLTATGTTEIDNVIADAAQPALAAPLPDQPETSDT
                     PTDSNSSIASWLFIGAAVILGGILAVILLRQRHS"
     gene            1287..2291
                     /locus_tag="PLC06_14715"
     CDS             1287..2291
                     /locus_tag="PLC06_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO91747.1"
                     /translation="MTARKVDIAIVGAGLAGLMAGRRLMESGLSVAIFESAPVVGGRI
                     MTHSVGKGRADSGAQFFTVREPEFAIIVAQWLRDGVVFEWTRGWSGGSLGGPIVDGYP
                     RYAAHNGFTALAEYLAKGLPIYRSCPIESLSVQDEQWVLAGPNPSSMVQARAALLCLP
                     VPEALSILAEGGVVLPEEERRALAAIRYGRCLCGLFEIEGGDELPFPGAVQRPDQAIT
                     WVADNRRKGVSANSRAITAHVGLAASEAYWSADDEEVLAWIWKEIGEWFSAATRITGA
                     RLVRWPYAVPLSTYPGRYLLGALAPLLAFAGDAFNGPRVEGAVLSGLAATGALLASLR
                     "
     gene            complement(2296..2964)
                     /locus_tag="PLC06_14720"
     CDS             complement(2296..2964)
                     /locus_tag="PLC06_14720"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRO91748.1"
                     /translation="MSFFRKGCLLSLATVTLLIVSPIAWRNAVKLYYQRSIYTRPEVP
                     EGQTAVVFGAAVYANGRLSPVLRDRVDTAIALYKAGKVGHIILSGDNREADYDEPGAM
                     MDYAVQQGVDSADITVDRAGHRTYDTCYRAGHVFDVKNAVLVTQEFHLPRALMTCEGL
                     GIKSVGAVADMRPYRGAGWYEIRETGATLVALWDVMRREPPPLMGLSGTNIQRTESQL
                     AAGN"
BASE COUNT          597 a          935 c          957 g          697 t
ORIGIN      
        1 cgaagccggt gtctatggcc atgagttcag cggctccacg cgtgattatt atcagttgtt
       61 gaaagtggcc tcgcaggtcg ccaaggcgaa cgatcctgag gctgttatcc atctggccgg
      121 ttggtcatac tggcatgatc ccacctggct ggcgcaattt ctgaatgttg ccacctccga
      181 tcctgaggga cctgccaacg gctacttttt cgatgccatc acgctccata tttatttccg
      241 cgtcgaaacg gtggaggagc ttgtccagga gacgtgggag attcagcgac gctatggcct
      301 gaagaaaccg atctggatta acgagaccaa cgcctcgccg aacctcgacc cgctctggcc
      361 ggtcgagcgg ccgaacttcc aggtcaacct ggagcaacag gcgtggtata tcgttcaggc
      421 tcacgcgctc ggctttgggg ccaatgccag ctcaatcggc gtatacaaat tgctggatat
      481 tttgctgcct gccggtggcg agtccttcgg catcttgcgt cccgatggtt cccgccggcc
      541 ggctttcgat gcctacaaga cgactatccg ttatctccgc gacttcacct ttccggttgg
      601 ccgtcagcag gagaaggatt tttattcatt caccttcaag cgccccgagg gattcacgcg
      661 cgtactgtgg gcgcgaaatt caaacgacca gatcttgaag gtgccggcga tcaccggcga
      721 ggcgcgtctg atctctgctc tcggtgacac ggagacgaca ctccgcgctt ataaggggtt
      781 ctatcgcatc cgcttgcgcg gtgctttgtg caacccggcg tgcgatattg gtggcgcgcc
      841 tgtgttcctc gttgaggaga cgagtgcgga cgttccagta gagagcgttc ctctgatctc
      901 gccgagagac tccattctgg cgaccccaac tcccactgcg gttgtcactg cgacgataat
      961 gcctgacggc acggtcacag ctgaggcgtc ccctgagccg actcagacgc agacagctac
     1021 accgcatccc tcggagacgc ctacgataga acctacggct acaaccgctc caacgctcac
     1081 ggcgacaggc acgactgaga tcgacaacgt gattgccgac gcagctcaac ctgcacttgc
     1141 cgcacctttg ccggaccaac ctgaaacaag cgacacgcct acggatagca actcttccat
     1201 cgcctcatgg ttgttcatcg gggcggccgt tatattgggc ggaattctgg ccgtgatact
     1261 cctccggcag cgacattcat agagctatga ccgcgcgtaa ggtagatatc gccatcgttg
     1321 gcgccggtct ggcgggcctg atggcgggac gtcgcctgat ggagagtggt ttgagcgtag
     1381 ctattttcga gagtgccccg gtggtgggcg gccgcatcat gacccattct gtcggcaagg
     1441 ggcgtgccga ttcgggggcc caattcttca cggtccgcga acctgaattc gcgataattg
     1501 tggcgcaatg gctgcgggac ggagtggttt ttgaatggac gcggggctgg tcgggcggct
     1561 cccttggagg gccgattgtt gacgggtatc cacggtatgc tgcccacaac ggatttactg
     1621 cgctggctga atatttggca aagggtctgc cgatctatcg ctcctgtccg attgaatctc
     1681 tctctgttca ggatgagcag tgggtcttgg ccggcccaaa cccgtcgtcg atggttcagg
     1741 ccagggcagc cttgttgtgc ctgccggttc ccgaagccct gtcgatcctg gcagaaggtg
     1801 gggtagtgtt gcccgaagag gaaagacggg cacttgccgc gattcgctac ggccgttgcc
     1861 tttgtggcct gtttgagatt gaggggggcg atgaattgcc gttcccggga gccgtgcagc
     1921 ggcctgacca ggccattacc tgggtagccg ataatcgccg aaaaggggtt tcggccaact
     1981 caagggccat tactgcgcat gttgggcttg cggccagcga ggcatactgg tcggcggatg
     2041 atgaagaagt attggcctgg atatggaagg agatcggcga gtggttctcg gccgccacgc
     2101 gaataaccgg cgctcgcctc gttcggtggc cctacgcagt gcctctgagc acgtatccgg
     2161 ggcgttattt gcttggcgcg ttggccccgc tgctcgcgtt tgcgggcgac gcgttcaatg
     2221 ggccgcgcgt ggaaggcgcc gtgctgtccg gcctggcggc caccggcgca ttgcttgcaa
     2281 gcttgcgcta gttcgtcaat tccctgctgc taattgggat tcggtgcgct gaatattcgt
     2341 cccggacagc cccatcaacg gtggtggttc gcgccgcatg acgtcccata aggccaccag
     2401 cgtcgcgccc gtttcccgga tctcatacca ccccgcgccg cgatagggtc gcatatcggc
     2461 cactgcgccg actgacttga tccccaatcc ttcgcaagtc atcagggcgc gaggcaagtg
     2521 aaactcctgc gtcacaagca cggcattctt cacatcgaag acgtgccctg cgcggtagca
     2581 ggtatcgtag gtgcgatgtc cagcccgatc gaccgtgatg tcggccgagt ccacgccctg
     2641 ttggacggca taatccatca tcgcgcccgg ctcatcgtag tccgcctcgc gattgtcccc
     2701 actcaggatg atatgtccca ccttgccggc tttgtagagc gcgatagccg tatctacccg
     2761 gtcgcgcagc acggggctga gtcgtccatt ggcgtaaact gccgcgccga atacaaccgc
     2821 tgtctggcct tcgggcactt cggggcgggt gtagattgag cgctggtagt acagcttgac
     2881 ggcattgcgc catgcgatcg gcgatacgat cagcagtgtg accgtcgcca gagacaggag
     2941 gcagcctttg cggaaaaatg acatcgaccc gattatagga cgacaaagcg ccgctctggc
     3001 gccggttagg cctcctctaa ggaacctctg acgcacggct aaggattcat caatgatgaa
     3061 atggttatag cgaacatgcc ctgattttgt ctgcttgctc ggcaccgaac cggcgtttga
     3121 cgagccataa tcgagcggct acactcccca gccacaggat tttgactaat gcgtaatcaa
     3181 cctcat
//
LOCUS       DAOMQG010000374         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12367_length_3177_cov_6.079436, whole genome shotgun sequence.
ACCESSION   DAOMQG010000374 DAOMQG010000000
VERSION     DAOMQG010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12367_length_3177_cov_6.079436"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLC06_14725"
     CDS             <1..810
                     /locus_tag="PLC06_14725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HRO91749.1"
                     /translation="YAQAAEALGYSHVLAYDHILGANPDRPGGWNGPYTFRDPFMEPF
                     LLFTYMAAVAPRLGFITGILILPQRETTLVAKESAVLDVLCGGRLRLGVGTGWNEVEY
                     AAAGYDFHTRGKRQEEQVEVLKLLWTQPLVTFKGRWHDIPDAGLNPLPVQRPIPVWFG
                     GHADAVLRRVARLGDGWLPGFRTAEAAAAHLDTLDRYLAEHDRGRADIGIEPRLHWGN
                     GDLDALGRALEGWRAAGATHISLNTMGSGFRTAEEHLAAIRQFALTYQMKN"
     gene            complement(807..1217)
                     /locus_tag="PLC06_14730"
     CDS             complement(807..1217)
                     /locus_tag="PLC06_14730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRO91750.1"
                     /translation="MTVSPQLRRLVTARANSRCEYCLLPEGDSTIKHAVDHIRAIKHN
                     GLTEEGNLALACIVCNQNKGSDIATIDPESRQLVRLFNPRSDIWGEHFALDGAVIVGL
                     TGVGRGTAEILKLNTPTRVKHRALLQSTNRYPKH"
     gene            complement(1243..1605)
                     /locus_tag="PLC06_14735"
     CDS             complement(1243..1605)
                     /locus_tag="PLC06_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91751.1"
                     /translation="MATITLEVPDELALQLEQIADQLPEMLATILEGGSLSIPSDAFV
                     ANQAWGEVLEFLAQVPDVQSILDFKLSDKLQDRIEELLFLGSEGTQTPQEREELDGYI
                     QVIQFFDLLKAQLRARIA"
     gene            1631..2875
                     /locus_tag="PLC06_14740"
     CDS             1631..2875
                     /locus_tag="PLC06_14740"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRO91752.1"
                     /translation="MELLGGPLFSVAGAGESHGPGIVTIVFGCPPGLWLTRAAVQAQL
                     DRRRPGSSRHGTPRREADRVAFLAGIYQDDHERLLEGPDAAVGDERVATYEAGYTTGE
                     PIAAVVLSAAGKSGHYAQFEGPEGEVRPGHTDLVKYHQSGGLVDIRGGGRSSYRATIS
                     DVIGGSIARLYLEATFGTVFLSSVVQVGPLRAARTLADAVRGLGPTTPPEQIKPLADA
                     LAWSWGEIGIPAVDTEFALEAADLIKRTRVAGDSLGAAVEVVALNVPALAGDPLYQSL
                     KLRLMGSLGGLHAVQACEIGDGAAVAGRLGSENNDPIRATGYAGNSHGGRIGGITTGN
                     PLVARVCFKPTSSIVKPQQSVRKNLEEIDFELKKGRHDPCVGVRAGVTLESRMAIELL
                     NAVLSHAARQVRPDDFTLFPSE"
BASE COUNT          578 a         1056 c          966 g          577 t
ORIGIN      
        1 tatgcgcagg cggccgaagc cctcggctac agccacgtgc tggcctacga ccacatcctc
       61 ggcgcgaacc ccgaccggcc cggcggctgg aacggcccgt ataccttccg cgacccgttc
      121 atggagccgt tcctgctgtt cacttacatg gctgccgtcg ctccgcggct ggggttcatc
      181 accggcatcc tcatcctgcc gcagcgcgag acgacgctcg tggccaagga gtcggccgtg
      241 ctcgacgtgc tgtgcggcgg ccggctgcgg ctgggcgtcg gcaccggctg gaacgaggtc
      301 gaatacgccg ccgccggcta cgacttccac acccgcggca agcggcagga ggagcaggtc
      361 gaggttctca agctgctgtg gacgcagccg ctggtcacgt tcaaggggcg ctggcacgac
      421 atccccgacg ccgggctgaa cccgctgccc gtgcagcggc cgatccccgt ctggtttggc
      481 ggccacgccg acgccgtgct gcggcgcgtg gcgcggctgg gcgacggctg gctgcccggc
      541 tttcgcaccg ccgaagcggc cgccgcgcat ctggacacgc tcgaccgcta cctggccgaa
      601 cacgaccgcg gccgcgccga tatcggcatc gagccgcgcc tccattgggg caacggcgac
      661 ctcgacgcgc tcggccgcgc gctggagggc tggcgcgccg ccggggccac gcatatcagt
      721 ttgaacacga tggggtcggg gttcaggacg gccgaagagc accttgcggc gatacgtcag
      781 ttcgcactga cttatcagat gaaaaattag tgttttggat agcggtttgt agactgtaag
      841 agagcccgat gcttgacccg tgtcggcgtg ttgagtttta gtatctcagc cgtgccacgc
      901 cctacaccag tcagaccaac gatcactgcg ccatccaggg caaagtgttc gccccatatg
      961 tcactacgcg gattaaacaa gcgcacgagt tgccggcttt ccggatcaat agttgcgatg
     1021 tcgctaccct tgttttggtt gcaaacaata caagcgagag caaggttccc ctcttccgtt
     1081 agcccattgt gctttatggc gcgaatatgg tcaactgcat gtttgatagt tgagtctccc
     1141 tcaggcagca gacagtactc acaacggcta ttggcgcgag cggtcacgag ccgtcgcaat
     1201 tgaggtgaca cagtcatgcg agtcatacag aagacaactc gtctatgcta ttctggcgcg
     1261 taactgggct tttagtaaat caaagaactg aataacctga atataaccgt ccaactcctc
     1321 ccgctcctga ggcgtttggg ttccctcact tcccaaaaat agcaattcct cgatacggtc
     1381 ctgcaacttg tccgacaact taaagtccaa aatgctctgg acgtcgggaa cctgtgccag
     1441 aaattcaagg acctctcccc aggcttgatt cgccacgaag gcatcggaag ggatactgag
     1501 gctccccccc tctaaaatag ttgccaacat ttccggaagt tgatcagcta tttgctctaa
     1561 ttgtaaagcc aactcatcag ggacttcaag cgttattgtt gccatggcca tagtatagca
     1621 taattcaggc atggaacttc ttggcggccc cctcttctcc gtcgccggcg cgggcgaatc
     1681 ccacggcccc ggcatcgtca ccatcgtctt cggctgccca cccgggctgt ggctgacgcg
     1741 cgcggccgtg caggcccaac tcgaccgccg ccgccccggc agcagccgcc acggcacccc
     1801 ccgccgcgag gccgaccgcg tggccttcct cgccggcatc tatcaggacg accacgagcg
     1861 cttgctggaa gggccggatg cggccgtcgg cgacgaacgg gtggccacct atgaggcggg
     1921 ctataccacc ggcgagccga tcgccgctgt cgtcctctcg gccgccggta aatcgggtca
     1981 ctatgcgcag tttgaaggcc ccgaaggcga ggtgcgcccc ggccacaccg acctcgtgaa
     2041 gtatcatcaa tcgggcgggc tggtcgacat tcgcggcggc ggccgctcca gctatcgcgc
     2101 taccatcagc gacgtcatcg gcggcagcat cgcccggctc tatcttgagg ccacgttcgg
     2161 cacggtcttt ctgtcgtcgg tcgtccaggt cgggccgctg cgggcggcgc gcaccctggc
     2221 cgacgccgtg cgcggacttg gccccaccac ccctccggag cagatcaagc cgctggccga
     2281 cgcgctggcc tggtcgtggg gggagatcgg cattcccgcc gtcgacaccg aatttgccct
     2341 ggaagcggcc gacctgatca agcgcacccg cgtggccggg gactcgctcg gcgcggccgt
     2401 ggaggtcgtg gcgctcaacg tgcccgcgct ggccggcgac ccgctctacc agagtctcaa
     2461 gctgcggctg atgggcagtc tggggggact acacgccgtg caggcctgcg agatcggcga
     2521 cggcgcggcc gtggccgggc ggctgggcag cgagaacaac gaccccatcc gcgccacggg
     2581 ctacgcgggc aacagccacg gcggccgcat cggcggcatc actaccggca acccgctggt
     2641 ggcccgcgtc tgtttcaagc cgaccagctc catcgtcaag ccgcaacaat cggtgcgcaa
     2701 gaatctggaa gagatcgatt ttgagctgaa gaaggggcgg cacgatccct gcgtcggcgt
     2761 gcgcgccggg gtcacgctgg agtcgcgcat ggccatcgag ctgctcaacg ccgtgttgag
     2821 ccacgccgcg cggcaggtgc gacccgacga tttcaccctc ttcccgtccg agtgacccat
     2881 ccggcggccg gccgattgtc acaattgcct aactgagagc ctcgttagtc aggatgcctt
     2941 gcgcagttac agggcgtcgc cgctatgcta tactgccttt atcggatcgc tcaacgtccg
     3001 aagttcgcca accttaatcc atgaacccag gccacggcgg gagctgccgc ccggacaggc
     3061 cactcatacc atcatcaatt ttgtcttgtc cctgccccgg cgacggccga agcagagggt
     3121 catttaattt ggaaagccgg ccatgaacct gttactctac accgaagcca gaatgaa
//
LOCUS       DAOMQG010000375         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12422_length_3165_cov_10.745659, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000375 DAOMQG010000000
VERSION     DAOMQG010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12422_length_3165_cov_10.745659"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..847
                     /locus_tag="PLC06_14745"
     CDS             389..847
                     /locus_tag="PLC06_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91753.1"
                     /translation="MARYGYGVLYPDTWTVRTRSATYGIVHETTFLPPTDGPAMGVVV
                     DVWKPPEDPAFDLLEWVNLNSDATIRLVLDEPLTYNAIALGRPAVYYFHADDGRTTNM
                     AQVLFATEQGRFRVTMFAPSPLSEEVMPIYRAMLASFTAAGEAEMGVVIP"
     gene            958..1638
                     /locus_tag="PLC06_14750"
     CDS             958..1638
                     /locus_tag="PLC06_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91754.1"
                     /translation="MKNATLLGFDGDGVGVFHLQSTERVARVGDEVVVSGGRIIRDDA
                     GTDKFRRSLTRPVAHSILRSHTILKMLGRARGQWVMKNIFRNPDGEVNTQIVVAAIGA
                     TATLLTALIAGIFGLIQLQASRAQTPPTPTVNAFTVEIDGPTEAPLNEETWFTIISED
                     AERVEWTIAGFGSDEIDPFERSDQIYVEPVDAGRVGEQFTLVVTAYDDGNRATARHEF
                     QITDASSP"
     gene            1653..2876
                     /gene="chrA"
                     /locus_tag="PLC06_14755"
     CDS             1653..2876
                     /gene="chrA"
                     /locus_tag="PLC06_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRO91755.1"
                     /translation="MTEQLITPAPAVNAPQESYVRLFLRFLRFGLLAWGGPVAQIALI
                     RQELVEEEQWVSRERFNRVLGVYQILPGPEATELCVYFGMLSRGRIGGTVAGLGFVLP
                     GFVLMLLLSWFYVTYGINSPVFSAAFLGMQAAVGALIVRAVHRIGSHVLHNRWLWAIA
                     VLAAITHFAGIHFLLNLSIAGLIYLLMKQGRDKLSTVLLLTVGVTSLALLIARLSVGQ
                     IALEAAGAISEDHPTTLSLFALGLRSGLLTFGGAYTAIPYLRNEAVVVSGWMTDGQFL
                     DGLALAGILPAPLVIFGTFVGYLGGGLLGAIALTLGIFLPAFGMTLIGHELLEKVVEN
                     KAFHSILDGVTAGVVGLIAATAVVLLRDAISGWLSLLIFLGGLMILFRWNSKINVPVV
                     VLGAALVGLLAYFLL"
     gene            complement(2896..>3165)
                     /locus_tag="PLC06_14760"
     CDS             complement(2896..>3165)
                     /locus_tag="PLC06_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO91756.1"
                     /translation="NAPEPTAVVRAAAERVLGTENILEHRTMAAEDMGFILEEIPGCY
                     FFVGCSNGNPATQFPHHHPRFDFDERAMVNGVAIMAEAAGAYVLI"
BASE COUNT          562 a          957 c          947 g          699 t
ORIGIN      
        1 ggggatattt tggtactgga ataacacacc gactgtcatc cataaggagt gtcttaagtc
       61 acttgcgacc atgattcaaa gccgctacca tgaacgtatg atcaagctgc gcacaatgtt
      121 cgcattggca agttgcgtcg cggccctggg tcttctggtc gcgtgtactc cagtcacccg
      181 gtctccattc tccactccac cgttaccatc gaccgcgacg gctacccctg ttccagagac
      241 gacgtcggaa ccgacggccg cggccatacc gcccgctacg tcgaccgtca tcgctccaac
      301 gtcaacgcca accgcgatcc cttcgccgcg cgcaccggta tcaacactaa ctccgccgcc
      361 cctgccccct ggttgggcct ggcacgcagt ggcccgctac ggctacggcg tgctgtaccc
      421 cgacacctgg acagtgcgga ctcgctcagc cacctatggc attgtccatg agacaacctt
      481 tctgccaccg acggatggtc cggccatggg agtggtggtc gacgtctgga aaccacccga
      541 agatcccgcg ttcgacctgt tggagtgggt caacctcaac tccgacgcca cgatccgcct
      601 agtcctggat gagccgttaa cctacaacgc catcgcgctg ggtcgccctg ccgtctatta
      661 cttccatgcc gatgacgggc gcacgaccaa tatggcgcag gttctgttcg ccaccgagca
      721 aggtcgcttt cgcgtcacga tgttcgcccc atcgccgctg agcgaggagg tcatgccgat
      781 ctatcgggcg atgctggcaa gctttaccgc ggccggagag gcagagatgg gggtggtaat
      841 tccctagaac tgtcgtggac aatgcaacac gcgaggagca aacgcacccc actttctgcg
      901 tggtcatact ataattatcc gttaggtaga aatgcacgcc attccggagt gtcgaggatg
      961 aagaacgcta cgctgcttgg gtttgacggc gacggggtgg gagtatttca cctgcaaagc
     1021 acggagcgcg tggcgcgcgt gggcgacgag gtggtggtca gcggcggccg catcattaga
     1081 gacgacgccg ggaccgacaa gttccgccga tcgttgacgc gacccgtggc ccactctata
     1141 cttcgctccc acaccatcct gaagatgctc ggccgagcgc gaggacagtg ggtcatgaaa
     1201 aacatcttcc gcaaccccga cggcgaagtg aatacccaga tcgtcgtcgc cgccatcggc
     1261 gcgacggcga cgctgctgac cgcgctcatc gccggcattt tcgggctgat ccagcttcag
     1321 gcttcacgcg cccaaacccc gcctacccct accgtcaatg cgttcaccgt cgagatcgac
     1381 ggcccgactg aagcgccgct gaacgaggag acctggttca ccatcatcag cgaggatgcc
     1441 gagcgcgtgg aatggacgat tgccggcttc ggcagcgacg agatcgatcc atttgaacga
     1501 agcgatcaaa tatacgttga gcctgtggat gccggacgtg tgggagaaca attcacgctg
     1561 gtcgtgaccg cctacgatga cggaaaccgc gccactgccc gccatgagtt ccagatcacc
     1621 gatgcctcat caccgtagca aaatcaccct gtatgactga gcagcttatc acccctgccc
     1681 cagccgttaa tgccccacaa gagtcttatg tgcgcctgtt cctgcgtttt ctgcgctttg
     1741 gcctgttggc ctggggcggc cccgtcgccc agatagctct aatccgccag gagttggtgg
     1801 aggaagaaca atgggtcagc cgcgagcgct tcaaccgtgt gctgggcgtc tatcaaattc
     1861 tccctgggcc ggaggccacc gaactttgcg tctatttcgg gatgctgtcg cgcggccgga
     1921 ttggcgggac ggtggccggg ctggggtttg tgctgcccgg cttcgtcctg atgctgttgc
     1981 tgtcgtggtt ctatgtgacc tacgggatca actcgccggt cttctcggcc gcttttctgg
     2041 ggatgcaggc ggcggtcggc gcgttgattg tccgggcggt ccaccgcatc ggcagccacg
     2101 tgcttcacaa ccgatggctt tgggcgattg ccgtcctggc tgccattacg cacttcgccg
     2161 ggatccattt tcttctgaac ctgagtattg ccggcctgat ttatctgttg atgaaacaag
     2221 gccgcgacaa gttgagcacg gtattgctcc tgaccgttgg ggtgacgtca ctggccttat
     2281 tgatcgcccg cctaagcgtg gggcagatcg cgcttgaggc cgccggcgct atttccgaag
     2341 atcaccccac aaccctttcc ctgttcgcat tgggactgcg cagtggtttg ctgacttttg
     2401 gcggcgccta taccgcgatc ccctatctgc gtaatgaggc ggttgttgtc agtggctgga
     2461 tgaccgacgg ccagtttctc gatgggctgg cgttggccgg cattttgccc gccccgctgg
     2521 ttattttcgg cacgtttgtg ggttatctgg gtgggggtct gttgggggcg attgctctaa
     2581 cgttgggtat cttcctgccc gctttcggga tgactctaat cgggcatgaa ctgcttgaga
     2641 aagtcgtcga aaacaaggcg ttccatagta ttcttgatgg cgttacggcc ggggtagtcg
     2701 gtctgatcgc cgctacggct gtggtgttgc ttcgggatgc gatctccggt tggctctccc
     2761 tgctcatctt tcttggcggc ctgatgatac tcttccgctg gaactcaaaa ataaacgtgc
     2821 cggttgtcgt tctgggggcg gcgctcgtcg ggcttctggc ctattttctg ttgtgaccga
     2881 gcttcaggga ttagatcaaa taagcacgta agcgccggcg gcttcggcca tgatcgccac
     2941 gccgttaacc atggctcgct cgtcgaagtc gaaacgcggg tggtggtggg ggaactgcgt
     3001 ggccgggttc ccgttgctgc agccgacgaa gaagtagcag ccggggatct cctccaggat
     3061 gaagcccatg tcctcggcgg ccatcgtgcg atgctccagg atattctctg tgcccagcac
     3121 gcgctcggcc gcggcgcgaa cgacggccgt cggttccggt gcgtt
//
LOCUS       DAOMQG010000376         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12554_length_3138_cov_6.676938, whole genome shotgun sequence.
ACCESSION   DAOMQG010000376 DAOMQG010000000
VERSION     DAOMQG010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12554_length_3138_cov_6.676938"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..661
                     /gene="folE"
                     /locus_tag="PLC06_14765"
     CDS             59..661
                     /gene="folE"
                     /locus_tag="PLC06_14765"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRO91757.1"
                     /translation="MTDGPVLSARCKGVVADSVAQILRAVGEDPEREGLLKTPERVAR
                     AYDELLIGYRLDPLAELNNALFDVTYREMVIVTDIDFYSLCEHHMLPFIGKAHVAYIP
                     DKKVVGLSKIPRIVEIYARRLQIQERMTRQIAELIEELLHPLGVAVVIDGMHMCMAMR
                     GVKKANARMRTSALRGAFAENTKTRSEFMEHIGPVTRTLL"
     gene            complement(658..1056)
                     /locus_tag="PLC06_14770"
     CDS             complement(658..1056)
                     /locus_tag="PLC06_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HRO91758.1"
                     /translation="MTLVDSSVWIDFFNGRGSPEALYFKDNLRRERFLVADLVLLEVL
                     QGFRGDLEFERARDAMLKFQIVSLGGRQAALRSAQNYRRLRAQGVTVRSAIDCLIASF
                     CIANECRLLHSDRDYSYFAAHLGLKVVGVS"
     gene            complement(1043..1252)
                     /locus_tag="PLC06_14775"
     CDS             complement(1043..1252)
                     /locus_tag="PLC06_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRO91759.1"
                     /translation="MRTNIVIDDELMAEAMELTGLKTKRAVVEKALQALIQLNRQGEI
                     EDLFGRLHWEGDLNRIREDRVDYAG"
     gene            complement(1322..2212)
                     /locus_tag="PLC06_14780"
     CDS             complement(1322..2212)
                     /locus_tag="PLC06_14780"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO91760.1"
                     /translation="MNESIGFIGLGIMGSGMAANLLRAGFNVTVWNRTPARITPLAGA
                     GAAVADNPAAVAESSTIIVTCVSDTPDVEEVILGPQGIINGVRSGSLVIDCSTISPNA
                     TKAIAAQLEARGAWMLDAPVSGGSEGAAKGTLSIMVGGDAEQVTRAMPVLQAMGKTIT
                     HVGGQGAGQMAKLVNQILVVVTMQAVGEALLFAQAGGLDLERTLAAVGGGAAGSWMLN
                     NRGPQVIRRDWRPGFTIDLQQKDLRLVLEAADQLGVPLPSTAQVFQNYRALQQLGLGG
                     EGNHALVKALERLAGIEIAA"
     gene            2384..>3138
                     /locus_tag="PLC06_14785"
     CDS             2384..>3138
                     /locus_tag="PLC06_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRO91761.1"
                     /translation="MGNRLRVGLIVVMAGVLLAAAAWWALPRLAASLPGRVRHYVPEA
                     LAGIGVTPLPAALPAPTGTPAIALAMENRAADGRPTTAASPTVSPDNGTEPTPVATPT
                     AVGGQLSAVISPYARIADMPIIPQKFNNCGPTNLTIVLNHYGIDVDQFDVAAVARPNY
                     EDRNVSPGELVAYVRERTALAAEAYAGGDVDLLRRLVAAGFPVIIEKGLEPDAKTGWM
                     GHYLTIFGYDDITGHFLARDTFLGPWHGDGMVS"
BASE COUNT          565 a          992 c          992 g          589 t
ORIGIN      
        1 tatggacaat caattgtatc tcgatgaagt cggcagtcag gatgattggg gcggcgcgat
       61 gacggacggc ccggtgttgt cggcgcgctg caagggcgtc gtcgccgatt cggtggccca
      121 gattttgcgc gccgtgggcg aagaccctga gcgggaaggg ctgctcaaga cgcctgaacg
      181 ggtggcgcgc gcctacgatg agctgctcat tggctatcgc cttgacccgc tggccgagtt
      241 gaacaacgct ctgttcgacg tgacctatcg tgagatggtc atcgtgaccg acatcgactt
      301 ctatagttta tgcgagcatc acatgctgcc gttcatcggc aaggcccacg tagcctatat
      361 ccccgacaag aaagtggtgg gcctgtcgaa aatcccgcgc attgtggaga tctacgcccg
      421 ccgcctgcaa attcaggagc gcatgacgcg gcagatcgcc gagttgatcg aggagctgct
      481 ccacccgttg ggggtggcgg tggtcatcga cgggatgcac atgtgcatgg cgatgcgcgg
      541 cgtgaagaaa gccaacgcac ggatgcgcac cagcgcgctg cggggggcgt tcgccgaaaa
      601 caccaagacg cgctcggagt ttatggagca catcggcccg gtgacgcgga cgctgcttta
      661 ggacacgccc acgaccttca ggcccaaatg tgccgcaaaa tacgagtagt cgcggtctga
      721 atggagcagg cgacactcat tggcgatgca aaagctcgcg atgaggcaat cgatcgcgct
      781 gcgaacggtg acaccttgtg cgcggagtcg ccgatagttc tgcgcgctgc ggagcgccgc
      841 ctgccggccg cccaatgaga cgatctggaa tttgagcatg gcatcacgcg cgcgctcaaa
      901 ttccaggtcg ccgcgaaatc cctgcaggac ttccagcagc acgaggtcag cgacgaggaa
      961 acgctcccgg cggagattgt ccttgaaata gagcgcttcg gggctgccgc ggccgttgaa
     1021 gaagtcgatc cacaccgagg aatcaaccag cgtaatcaac gcgatcctcg cgaatccggt
     1081 ttaaatcccc ttcccaatgg agccggccga agaggtcttc gatttcccct tgccgattca
     1141 attggatgag cgcctgcagc gccttttcga ccacggcccg cttggtcttc aggccggtca
     1201 attccatcgc ttcagccatc agttcatcgt cgatcacgat attggtgcgc attgattcgc
     1261 tattcccctt cgatatgtgt atagaataac gtattaatgt gtataaatca atgctgctgc
     1321 ctcaggccgc tatttctatt cccgccagcc gttccagcgc cttgacgagc gcgtgattgc
     1381 cttcgccgcc caggcccagc tgctgcaggg cgcggtaatt ttggaacacc tgcgccgtgg
     1441 aggggagggg gacgccgagc tggtcggccg cctccagcac cagtcgtaaa tccttctgct
     1501 gcaggtcgat ggtgaagccc ggccgccagt cgcggcggat gacctgcggg ccgcgattgt
     1561 tgagcatcca cgacccggcc gcgccgccgc cgacggccgc cagcgtccgc tccagatcga
     1621 ggccgcccgc ctgggcgaag agcagcgcct cgcctacggc ctgcatggtg acgacgacga
     1681 gaatctggtt gaccagcttg gccatctggc ccgcgccctg gccgccgacg tgggtgatgg
     1741 tcttgcccat cgcctgcagg acaggcatgg cccgcgtcac ctgctccgcg tcgccgccga
     1801 ccatgatgct gagcgtgccc ttggccgcgc cctcgctgcc gccgctgacc ggcgcgtcga
     1861 gcatccaggc gcccctggcc tccagctgcg cggcgatcgc cttcgtcgcg ttggggctga
     1921 tggtgctgca atcgataacc agcgaccccg aacgaacgcc gttgatgatc ccctgcggac
     1981 caaggatgac ctcttccacg tcgggggtgt cactgacgca agtgacgatg atggtgctgc
     2041 tttcggccac ggccgcggga ttgtcggcca cggccgcgcc ggctccggcc agcggtgtga
     2101 tgcgggcggg tgtgcggttc cagaccgtca cattgaagcc cgcccgaagc aagttggccg
     2161 ccatgccgct gcccatgatg cccagtccga taaagccgat tgattcattc atactgtatg
     2221 ccgcctgttt ttcataaatt tgggcgatta tgggacgggt gtcgggcatt gtcaacggaa
     2281 tgtagcgcaa tcattcctga ttgcgctctg cgccgagcga ggataagacg cagacaggaa
     2341 tgtctgcgct acggcaaatg ctgcggttat aatggatggc atcatgggta accggttacg
     2401 tgtggggctg attgtagtca tggcgggcgt cctgctggcg gctgcggcgt ggtgggcatt
     2461 gccgcggctg gcggcgagcc tgcccggccg cgtgcgccat tatgtccccg aagcgctggc
     2521 cggcatcggc gtgacaccgt tgccggcggc cctgcccgcg ccgaccggca ccccggcgat
     2581 cgcgctggcg atggaaaacc gggccgccga cggccgaccg acgaccgctg caagccccac
     2641 cgtgtcaccc gacaacggaa ccgaacccac tcctgtagcc actccaacgg cggtcggcgg
     2701 tcagctgtcg gccgtcatct ctccttacgc ccgtatcgcc gacatgccca tcatcccgca
     2761 gaagttcaac aactgcggcc ccaccaattt gaccatcgtc ctcaatcatt acgggatcga
     2821 cgtggatcag ttcgatgtgg cggccgtggc gcggccgaac tacgaggatc gcaacgtctc
     2881 gcccggcgaa ttggtggcct atgtgcgcga gcggaccgca ctggcggccg aggcctacgc
     2941 cggcggcgac gtcgacctgc tgcgccgtct cgtcgccgcc ggtttcccgg tcatcatcga
     3001 gaagggactg gagccggacg cgaagacggg ctggatgggc cactatctga ccatcttcgg
     3061 ctacgatgac atcaccggcc atttcctggc ccgcgacacc ttcctcggcc cgtggcatgg
     3121 cgacggcatg gtgagcta
//
LOCUS       DAOMQG010000377         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12575_length_3135_cov_7.441234, whole genome shotgun sequence.
ACCESSION   DAOMQG010000377 DAOMQG010000000
VERSION     DAOMQG010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12575_length_3135_cov_7.441234"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..282)
                     /locus_tag="PLC06_14790"
     CDS             complement(55..282)
                     /locus_tag="PLC06_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015117295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO91762.1"
                     /translation="MEELNRITFNPAVMGGKPTIRGMRVTVGTVVGLVASGYTTAEIL
                     AAYPYLEEEDISQALAYAAWRVEEVEIPLAL"
     gene            complement(367..3105)
                     /locus_tag="PLC06_14795"
     CDS             complement(367..3105)
                     /locus_tag="PLC06_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HRO91763.1"
                     /translation="MTRSTAHNVLRMEKNPLEVMFKPRSVAVIGATERQGSVARTILW
                     NLLSTSFGGTIFPVNPKYGSILGIKAYPSVGAIPDEVDLAVIVTPAKTVPGLIQECAD
                     KGVRAAIIISAGFKETGPAGVALEEEILKIARKSNMRIVGPNVLGVMNPITGLNATFA
                     STMARPGSVGFISQSGALLTAILDWSFRENVGFSAFVSIGSMLDVGWGDLIHYLGDDP
                     NTRSIVIYMESIGDARSFLSAAREVALTKPIIVIKPGRTEEAAKAAASHTGSLTGGDE
                     VLAAAFRRSGVLRVDTISDLFSMAEVLSKQPRPRGPRLTIVTNAGGPGVLATDALITG
                     GGQLAPVSPAAMEQYNKLLPPHWSHNNPIDILGDADAERYARSIEIAVNDEESDGTLV
                     ILTPQAMTNPTDTARALADHFARPAGHAYGKPILASWMGGPEVSSGESILNKANIPTF
                     AYPDTAAQTFNYMWRYQRRLTALYQTPELAEDYGETGFKHYLIKEMLDQIRATGRTIL
                     TEYESKQVLEAYDIPTVPTRLAKSADEAVRIAAEIGYPVVLKLNSETITHKTDVGGVR
                     LDLGNAEEVRNAYLTMERSVGEKYSPDDFLGVTVQPMVSLKNGYELILGSSPDPQFGP
                     VVLFGTGGTLVEVFKDRALAIPPLNTTLARLTMSRTKIYEALKGVRGRPPVDLAELDK
                     LFVRFSQLVVEQRWIKEIDINPLFVSHEQIIALDGRVVLYEPEVKEEDLPRLAIRPYP
                     THYVKEFANKAGEPFTLRPIRPEDEPKMVRFHEKLSEESVYLRYFRAFKLDHRVEHER
                     LTRICYVDYDRDIVLVVEWTDPETGTQEIVAVGRLTRLPNPTEAEFAMLVRDDFQGRG
                     IGTQLLQNLLSFGKDEGIERVVAWMLPANRGMIEISEKLGFKMAREEEMVKAVMEL"
BASE COUNT          509 a         1023 c         1004 g          599 t
ORIGIN      
        1 aattgagcca tgatgcccac gacggagaaa gattcatatc aattagcaat ttcatcacaa
       61 agccagagga atttccacct cttcaactcg ccaggcggca taggccaacg cctggctgat
      121 gtcttcctct tccagatatg gataggccgc gagaatctcg gccgtcgtat agccggacgc
      181 gaccagaccg acgacagtcc ccacggtaac gcgcatcccg cgaatggtgg gcttgccgcc
      241 catcacggcc ggattaaatg taatacggtt taactcttcc atgatagacc ccagtatacc
      301 acgagcaaca ccacaccatt cttcattccc aattcgtaat tcgtaattcg taattcgtaa
      361 tttcccctac aactccatca ccgccttcac catctcctcc tcccgcgcca tcttgaagcc
      421 cagcttctcg ctgatctcga tcatcccccg gttggcgggc agcatccagg ccacgacgcg
      481 ctcgatgccc tcgtccttgc cgaacgacag caggttctgc agcagctgcg tgccgatgcc
      541 gcgcccctgg aagtcgtcgc gcaccagcat ggcgaactcc gcctcggtcg ggttcggcag
      601 ccgcgtcagg cggccgacgg ccacgatctc ctgcgtcccc gtctccgggt cggtccactc
      661 caccaccagc acgatgtcgc ggtcgtaatc cacatagcag atgcgcgtca gccgctcgtg
      721 ctccacgcga tggtccagct tgaacgcccg gaagtagcgc agatagacgc tctcctcgct
      781 cagcttctcg tggaagcgca ccatcttcgg ctcgtcctcc ggccgaatcg gccgcagcgt
      841 gaacggctcg cccgccttgt tggcgaactc cttcacgtaa tgcgtcgggt agggacgaat
      901 ggccagccgc ggcagatcct cctccttcac ctccggctca tagagcacca cccgcccgtc
      961 gagggcgatg atctgctcgt gggacacgaa cagcgggttg atgtcgatct ccttgatcca
     1021 gcgctgctct accaccagtt ggctgaagcg cacgaacagc ttgtccagct cggccagatc
     1081 gaccggcggc cgcccgcgca cccccttcag cgcctcgtag atcttcgtcc gcgacatcgt
     1141 gagccgggcc agcgtcgtgt tcagcggcgg gatggccagc gcgcgatcct tgaacacctc
     1201 caccagcgtg ccgcccgtgc cgaacagcac caccggccca aactgcgggt cggggctgga
     1261 gccgaggatc agctcatagc cgttcttcag gctcaccatc ggctgcaccg tgacgcccag
     1321 gaagtcgtcg gggctatatt tctcgcccac ggatcgctcc atcgtcaaat aggcgttgcg
     1381 cacttcctcg gcgttgccca ggtcgagccg caccccgccg acgtcggtct tgtgggtgat
     1441 cgtctccgaa ttgagcttca gcaccaccgg atagccgatc tccgcggcga tgcgcaccgc
     1501 ctcgtcggcg ctcttggcca gcctcgtcgg cacggtgggg atgtcatacg cctccagcac
     1561 ctgcttggac tcgtactcgg tcaggatcgt ccggccggtg gcgcgaatct gatccagcat
     1621 ctccttgatc aggtaatgct tgaagccggt ctcgccgtaa tcctcggcca gctccggcgt
     1681 ctgatagagc gccgtgagcc gccgctggta gcgccacatg tagttgaacg tctgcgccgc
     1741 cgtgtcgggg taggcgaagg tggggatgtt ggccttgttg aggatggatt cgccgctgct
     1801 gacctccggg ccgcccatcc agctggccag gatcggcttg ccgtaggcgt ggccggcggg
     1861 gcgggcgaag tgatcggcca gggcgcgggc ggtgtcggtg gggttggtca tggcttgcgg
     1921 cgtcaggatg accagcgtgc cgtcgctttc ctcgtcgttg acggcgatct cgatggaccg
     1981 ggcatagcgc tcggcgtcgg cgtcgcccag gatgtcgatc gggttgttgt ggctccagtg
     2041 gggcggcagc agcttattgt actgctccat ggccgccggg gagacggggg ccagttggcc
     2101 gccgccggtg atcagcgcgt cggtggccag cacgccgggg ccgccggcat tggtgacgat
     2161 ggtcaggcgc gggccgcgcg gccgcggctg cttgctcagc acctcggcca tggagaacag
     2221 gtcggagatg gtgtcgacgc gcagtacgcc gctgcggcgg aacgcggccg ccagcacttc
     2281 gtcgccgccg gtcaatgagc cggtgtggga cgcggccgcc ttggccgcct cttcggtgcg
     2341 gccgggcttg atgacgataa tcggcttggt cagggccacc tcgcgcgcgg ccgagaggaa
     2401 ggagcgcgcg tcgccgatcg attccatgta gatgacgata ctgcgcgtgt tggggtcgtc
     2461 gcccaggtag tggatcaggt cgccccagcc cacgtcgagc atggagccga tggagacgaa
     2521 agcgctgaag ccgacgttct cgcggaaact ccagtcgagg atggccgtca gcagcgcgcc
     2581 cgactggctg atgaagccga cggagccggg ccgggccatg gtgctggcga aggtggcgtt
     2641 caggccggtg atcgggttca tcacgcccag cacgttgggg ccgacgatgc gcatgttgct
     2701 cttgcgggcg atcttgagga tctcttcctc cagcgccacg cccgcggggc cggtctcctt
     2761 gaagccggcg gagatgatga tggccgcgcg cacccccttg tcggcgcact cctggatgag
     2821 gccgggcact gttttggccg gggtgacgat gacggccagg tcgacctcgt cggggatggc
     2881 ccccacgctg gggtaggcct tgatgcccag aatgctgcca tacttggggt tgacggggaa
     2941 gatggtgccg ccgaacgagg tggagagcag gttccacagg atggtgcggg ccacgctgcc
     3001 ctgccgctcg gtcgcgccga tgacggccac gctgcgcggc ttgaacatca cttccagcgg
     3061 gttcttttcc atgcgcagca cgttgtgggc ggtgctgcgg gtcatatttt tgctgtcggt
     3121 cataaattcc tctcg
//
LOCUS       DAOMQG010000378         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12607_length_3128_cov_7.901074, whole genome shotgun sequence.
ACCESSION   DAOMQG010000378 DAOMQG010000000
VERSION     DAOMQG010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12607_length_3128_cov_7.901074"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLC06_14800"
     CDS             complement(<1..583)
                     /locus_tag="PLC06_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO91764.1"
                     /translation="MNLTENIRLAFSSLMANKLRAILTMLGIIIGVAAVITLVSVGEG
                     VQAVVVSEFQGLGNNLLFVTPGRLDAGEFGHSRTRAELTNDDYLTLTDTFNVPNLARV
                     APIYDRPALITRGGNEVQVTVTGTTPDYTAVRDFHPVLGDFFTEQDVTTAARVAVLGQ
                     TVYESLFPDGDVPIGQTIRINNVNFRVIGLMEEK"
     gene            complement(661..1428)
                     /locus_tag="PLC06_14805"
     CDS             complement(661..1428)
                     /locus_tag="PLC06_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91765.1"
                     /translation="MSNSNGTANGREPVILIKDMTKVYRMGEYEVHALNGVSLEIGAG
                     EFVAIMGPSGSGKSTLMNMLGALDQPTDGTYLLDGTDISAMSDDQLADIRNRKIGFVF
                     QNFNLLPRMPALQQVELPLIYAAKGQRHERARRALEMVGLGDRIDHRPSELSGGQQQR
                     VAIARALVNEPAIILADEPTGNLDSKSGTEVMAIFQRLNREQGITILFVTHDPWIARH
                     TRRIITLADGVVIRDEAVPEPLVAGKSARPSDEALVG"
     gene            complement(1452..3029)
                     /locus_tag="PLC06_14810"
     CDS             complement(1452..3029)
                     /locus_tag="PLC06_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRO91766.1"
                     /translation="MSRNLRNILLTVLVLALVGAGIYFFMQRRQAAAGQQSEVLREAT
                     ITRDRIASTVNATGALEPETLVTLTFGLSGTIVDVGVVRGQEVKADDVLAQLDTAEPA
                     LAVQQAEDALIIQQLTMAQRVDGEPSPAQLASSRADIQAAEANVQVAQANLASAQAGL
                     QQAQAQRAQLLAGASAGQIAQAEAQVSAARQQQKQAEETHNRTTECHTFTNPATNQEE
                     SVCPGLGAPEEQARAALEAATLNLQAAEASLADLNNPAGPADIQAADASIAGAQAQVQ
                     AAEGNVAAAEAQLARAHAAADLLVEKPAADEKAILEAQVASARTNLEIAQLRLRQAQI
                     IAPISGTVANVLIRAGEQAAPGSPAISILNEDAYHITVNVDEIDIDRVKVGQPVVVTL
                     DALPDQPVQGVVSEIAPTSTSTSGVVTYLVTINLDEAAAADLRPGMSASAAITVDELD
                     NVLVVPNWAVRLNRETGEAFALVRQADGTIDEVVIETGLRNEQFSEVISGLNEGDQVV
                     LTNEREGLSGFFPGGGG"
BASE COUNT          467 a          963 c         1103 g          595 t
ORIGIN      
        1 ctttctcttc catcaggccg atgacgcgga aattgacgtt gttgatgcga atggtttggc
       61 cgataggcac gtcgccgtcg gggaagaggc tctcataaac cgtctggccc aatacggcca
      121 cgcgggcggc cgtggtcacg tcctgctcgg tgaaaaagtc acccagcacc ggatggaagt
      181 cgcggacggc ggtgtagtcg ggcgtggtgc cggtgacggt cacctgaacc tcgttgccgc
      241 cgcgggtgat gagcgccgga cggtcgtaga tgggcgcaac gcgggccagg ttgggcacgt
      301 tgaacgtgtc ggtcagggtc aggtagtcgt cgttggtcag ctcggcccgg gtgcgggagt
      361 ggccaaactc acctgcatcg agccggccgg gggtgacgaa cagcaggttg ttgcccagcc
      421 cctggaactc ggagacgacc acggcctgca caccctcgcc cacggagacg agcgtgatga
      481 ccgcggccac accgatgatg atacccagca tggtcaggat ggcgcgcagc ttgttggcca
      541 tgaggctgct gaaggcgaga cggatgtttt cagtcaggtt catattgttt ttgaagggga
      601 gcaggggtga aagggtgcgg gggcgctccc cttctcccct tctccctttc tccctttctc
      661 ctatcccacc agcgcctcat ccgaaggccg cgcgctcttg ccggccacga gcggctcggg
      721 cacagcttcg tcgcgaatga ccacgccgtc ggccagggtg ataatgcgtc gcgtgtggcg
      781 ggcgatccac ggatcgtggg tgacgaacag gatagtgatg ccttgctcgc ggttcagccg
      841 ctggaagatg gccatcactt cagtgcccga cttgctgtcg aggttgcccg tcggctcgtc
      901 ggccagaatg atggccggct cgttgaccag ggcgcgggcg atggcgacgc gctgctgctg
      961 gccgccggac agttcgctcg gccggtggtc gatgcggtcg ccgaggccga ccatctccag
     1021 cgcgcggcgg gcgcgttcgt ggcgctgccc cttggcggca tagatgagcg gcagctccac
     1081 ttgctgcagg gcgggcatgc gcggcaacag attaaagttc tggaagacaa agccgatctt
     1141 gcggttgcga atgtcggcca actgatcgtc gctcatggcg ctgatgtcgg tgccgtccag
     1201 tagataggtg ccgtcggtcg gctggtcgag cgcgccgagc atgttcatca aggtcgattt
     1261 accggagccg gacggcccca tgatggcgac gaactccccc gcgccgatct ccagcgacac
     1321 gccgttgagg gcgtggactt cgtattcacc catccggtag accttggtca tatccttgat
     1381 gaggatcacc ggctcgcggc cgttggctgt tccgttgctg ttgctcatat taccccgatg
     1441 actcgaaagt cctagccccc accaccgggg aagaagccgc taagcccttc gcgctcgttg
     1501 gtcagcacca cctggtcgcc ctcgttcagg ccgctgatca cctcgctgaa ctgctcgttg
     1561 cgcaggccgg tctcgatgac gacctcgtcg atggtgccgt cggcctgacg caccagagcg
     1621 aacgcctcgc ccgtctcgcg gttcagccgc acggcccagt tgggcacgac gaggacgttg
     1681 tccagctcat cgacggtgat ggctgcgctg gcgctcatgc cggggcgcaa atcggcggcg
     1741 gccgcttcgt cgaggttgat ggtcaccaga taggtgacga cgcctgaggt ggacgtggac
     1801 gtcggggcga tctcgctgac gacgccttgc accggctggt cgggcagcgc gtcgagggtg
     1861 accaccaccg gttggccgac cttcacccgg tcgatgtcga tctcgtcgac gttgaccgtg
     1921 atgtggtagg catcctcatt caggatgcta atggccgggg agccgggtgc ggcttgctca
     1981 ccggcgcgaa tcagcacgtt ggccaccgtg ccgctgatgg gagcgatgat ctgcgcctgg
     2041 cgcagccgca attgggcgat ttccagattg gtgcgggcgc tggcgacctg ggcctcaagg
     2101 atggctttct cgtcggcggc cggtttctcg accagcaggt cggcggcggc atgggcgcgg
     2161 gccagttggg cctcggcggc ggccacgttg ccctcagcgg cctgcacctg ggcttgcgcc
     2221 ccggcgatgc tcgcatcggc ggcctggatg tcggccgggc cggcggggtt gttgaggtcg
     2281 gccagcgacg cctcggccgc ctgcaggttc agcgtggcgg cttccagcgc ggcgcgggct
     2341 tgctcttccg gcgcgccgag gccggggcag accgactctt cctgattcgt ggccggattg
     2401 gtgaaggtgt ggcactcggt ggtgcggttg tgggtttctt cggcctgctt ctgctgctgg
     2461 cgggcggccg acacctgagc ttcagcctgg gcgatctgcc ccgccgaagc tccggcgagc
     2521 aactgggcgc gctgggcctg ggcctgctgc agaccggcct gggccgaggc gaggttggcc
     2581 tgggccacct gcacgttggc ctcggcggcc tggatgtcgg ctcgtgacga ggccagttgc
     2641 gcggggcttg gctcgccgtc cacgcgctgg gccatggtca gctgctggat gatgagcgcg
     2701 tcctcggcct gttgcacggc cagcgccggc tcagcggtgt ccagttgggc cagcacatca
     2761 tcggccttca cttcctggcc gcgcacgaca ccgacgtcga cgatggtgcc gctgaggccg
     2821 aaggtgagcg tgacgagcgt ctccggctcc agcgcaccgg tagcgttgac ggtggaagca
     2881 atgcggtcgc gggtgatggt ggcctcgcgc agcacttcgg actgttgccc ggcggccgcc
     2941 tggcggcgct gcatgaagaa atagatgccc gccccgacga gggccagcac caggacagtt
     3001 aggagaatgt tcctgaggtt acgagacata ggttgttccg atccagaatt gatcccggct
     3061 cccggcatga ccgaggggcg acaggtcgat gatagcgatt aggtcatcat gctttgcgca
     3121 ttaaggcc
//
LOCUS       DAOMQG010000379         3118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12656_length_3118_cov_6.405158, whole genome shotgun sequence.
ACCESSION   DAOMQG010000379 DAOMQG010000000
VERSION     DAOMQG010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12656_length_3118_cov_6.405158"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..2390)
                     /locus_tag="PLC06_14815"
     CDS             complement(126..2390)
                     /locus_tag="PLC06_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91767.1"
                     /translation="MNRKFLGLIIVLLAAVVLPAQWATAGSALPNAVAPVSLIPGEPN
                     DTFGAAIPFDLYAHENAKGYFLTAADVDFYRIEAATPVELTAIATTGYAFGHTTMTAE
                     IAIYDATHTLVTQNTGCNDPAETITTLSEGTAFVRVRPCAAGLDIDKDYRLEIYADAL
                     EMEPNNTRQTANGGLAPYNEYVNYNVWQSGAVSPSGDVDFYRIQDAADQAFHAQVDAA
                     EGTAPLSLALMAADGTILAEGAPCWYDAGDSCMDTILPDGATWYLRVRSAAHPTGSGP
                     YSLYTVLEVEQWDIEPNSTPAEAIPVGYGTEIDVDGSDEGDVDYYRFNGQAGTKVRLL
                     ILEDFDMPYDAGLLGVELFDPSMNPVTLSGDPIFRGTLPSTGLYTLKITTTYDAGEYD
                     YLSVTLDRDKGSEPNDNRQAATPIEIGPELDIVFDYPCDTDWFVFNGRAGDALRSINT
                     EQVSENVRLYAADGTPMTGHVLPADGTYYLRAQGYYSDKWDNYCYEGPETHPFGEALW
                     VSPAANKFAGDKLLSSGDIVTRKSAAGQWQIVFDASDVGITKNVVAIERMPNGSILMS
                     LSAAQNVAGLGKVQPQDIIRFNPTSLGDTTAGTFEWFLDGSDVGLTTAGEKIDAIYMQ
                     QDIENPLRISLAGSGSVARVSGGTLKVADEDILNLVGGVFGANSAGAWRMGLDGSTVP
                     GLAKEDISNLARVEVYPAHDSVLLLGLDSAFTVNGTSGTMFDVMNGDTWLPAVRQLTN
                     KKIDGLAVGPAQTP"
     gene            2829..>3118
                     /locus_tag="PLC06_14820"
     CDS             2829..>3118
                     /locus_tag="PLC06_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRO91768.1"
                     /translation="MSPSALPYVLLTGLLFGSSLIVSRYAVGQMEPTTYIWLRLAISL
                     ILFAGVYAGAALRGRPRRLPAGRALWRDGVMLGVFSTALPMIGTIASLRYLSA"
BASE COUNT          576 a          997 c          891 g          654 t
ORIGIN      
        1 acgcttcatg attttcctca aaacatctct acgagatacg agatacgaga tacgagatac
       61 gagatacgag atacgagata cgagcgttct tcattcgtaa ttcgtaattc gtaattcgtc
      121 attgtttacg gtgtctgcgc cgggccgaca gccagaccgt cgatcttctt gttcgtcagc
      181 tgccgcaccg ccggcagcca cgtgtcgccg ttcatcacat cgaacatcgt gccgctcgtg
      241 ccgttgacgg taaacgcgct atccagcccc agcagcagca ccgaatcatg ggcgggataa
      301 acctccacgc gagccagatt gctgatatct tcctttgcca gccccggcac tgtcgagccg
      361 tcgaggccca tgcgccatgc cccggccgaa ttcgcgccga aaacgccgcc gaccaggttc
      421 aggatatctt cgtcggccac ctttagcgtg ccgccgctga ctctcgccac ggagccggag
      481 ccggccaggc tgatgcgcaa tgggttctcg atgtcctgct gcatatagat ggcgtcgatc
      541 ttctctcccg ccgtcgtcaa ccccacatcg gagccgtcga gaaaccactc gaacgtcccg
      601 gccgtggtgt cgcccagcga cgtcgggttg aagcggatga tatcctgcgg ctgcaccttg
      661 cccagaccgg ccacgttctg cgccgcggac aggctcatca ggatcgagcc gttgggcatc
      721 ctttcgatgg cgacgacgtt tttggtgatg cccacgtcgg aggcatcgaa aacgatctgc
      781 cattgaccgg cggccgattt gcgggtaacg atatcgccgc tgctgagcag tttgtcgccg
      841 gcgaatttat tggcggctgg gctgacccac agcgcctcgc caaagggatg tgtctccggt
      901 ccttcgtagc aataattatc ccacttgtcg ctgtagtacc cctgtgcccg caggtaatag
      961 gttccgtcgg ccggcagcac atggccggtc atgggggttc catcggccgc gtagaggcga
     1021 acgttctcgc tcacctgctc ggtattgatc gaccgcagcg cgtccccggc gcggccattg
     1081 aacacgaacc agtccgtgtc gcatgggtag tcgaaaacaa tgtccagctc cgggccgatc
     1141 tcgatcggtg tcgcggcctg gcggttatcg ttcggctcgc tgcccttgtc gcggtcgagt
     1201 gtgacgctga ggtaatcata ctccccggca tcataggtcg tggtgatctt caaggtatac
     1261 agcccggtgg aaggcagcgt gccgcgaaag atggggtcgc cgctgagggt caccgggttc
     1321 atcgacgggt cgaacagctc gacgcccagc aaaccggcgt catagggcat atcgaaatct
     1381 tccaggatca gcagtcgtac ctttgtgccg gcctggccgt tgaacctgta gtaatccacg
     1441 tccccctcat cgctgccgtc cacgtcgatt tccgtgccat aaccgacggg gatcgcctcg
     1501 gccggggtgc tgtttggctc gatgtcccat tgctcgactt ccagcaccgt atagaggcta
     1561 tacgggccgc tgccggtcgg atgggcggcc gagcgcacgc gcagatacca ggtcgcgcca
     1621 tcgggcaaga tggtgtccat gcagctatcg ccggcgtcat accagcacgg cgcgccttcg
     1681 gccaggatcg tgccgtcggc ggccatcagc gcgagggata gcggggccgt gccttcggcc
     1741 gcgtccacct gcgcatgaaa ggcctgatcg gccgcgtcct ggatgcgata gaaatcaaca
     1801 tctccgctcg ggctgacagc cccggattgc cagacgttgt aatttacata ctcattgtag
     1861 ggggccaacc cgccattggc tgtctggcgc gtgttgttcg gctccatctc gagcgcgtcg
     1921 gcgtagattt caaggcgata atccttgtcg atatcaagcc ccgcggcaca gggccgaacg
     1981 cgcacaaacg ctgtgccttc ggatagggtg gtgatggttt cggccgggtc gttgcaaccg
     2041 gtgttttggg tcaccaatgt gtgcgtcgcg tcatagatgg cgatctcggc cgtcatggtt
     2101 gtgtggccaa aggcatagcc tgtggtcgct atggcggtca gttcgacggg agtcgccgcc
     2161 tctatgcgat agaaatcgac atcggcggcc gtcagaaagt aaccttttgc gttctcatgg
     2221 gcgtaaaggt caaagggaat ggcggccccg aacgtgtcgt tgggctcgcc gggaatcaag
     2281 gaaacaggcg cgaccgcatt cggcaaagcc gatccggccg tcgcccactg cgccggcagc
     2341 acaacggccg ccagcagcac gattatcaac cccaaaaact tgcgattcat gattaggctc
     2401 cacaaactgc taacaaacca gtaactgtca accgcggttg acacttctcg cccctggcgc
     2461 ggagcgaatc aagcctgcgg gcaacgcgaa ctcggccgcc caacgacaaa gaccgatccc
     2521 ggtttttaat gtattcggat tgtgactcca cgttcgccgc aaaggacgaa catctgcccc
     2581 ggttccgcgc acgggcctgg catgcccgtc gacggaatgg ccgcgaaccc aaggctcaaa
     2641 ccgaccatga ctgacggcac ggatggtttc gtggtacgca tccttgtgcc atcgcctcta
     2701 tttaccagta tactcactcc caacccggct gctataaact agtttacact ttcccccaat
     2761 tcctgttccc aattcccaat tccaattccc aattcgtaat tcgtaattcg taattcgtaa
     2821 ttctccccat gtccccatcc gccctgccct atgtcctgct caccggcctg ttattcgggt
     2881 cgtcgctcat cgtctcccgc tatgccgtcg gccagatgga acccacgacc tacatctggt
     2941 tgcggctggc catcagcctg atcctctttg ccggggtcta tgccggagcc gccctgcgcg
     3001 gccggccccg ccgcctgccg gccggccgcg cgctgtggcg cgacggcgtc atgctgggcg
     3061 tcttcagcac cgccctgccc atgatcggca ccatcgcctc gctgcgctac ctgtcggc
//
LOCUS       DAOMQG010000380         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12786_length_3093_cov_12.902897, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000380 DAOMQG010000000
VERSION     DAOMQG010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12786_length_3093_cov_12.902897"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1504)
                     /locus_tag="PLC06_14825"
     CDS             complement(245..1504)
                     /locus_tag="PLC06_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRO91769.1"
                     /translation="MTDLNDPLSDEMEILNALLNEGIEQRYLTYDQILEALPEIENNI
                     TLLETLLEEAQTLGLVIYENEEDIVAAALIVDGDDEDLEADLDDEAPAPRETRVVNPA
                     RVRSHDAPLFDLSNVPIDDSVGLYFREMGQQSLLSADEEVHLAMEIEAGRAAVERLES
                     VEELDLDARDLLERQREVGDAARAHLIRANTRLVVSIAKKYRGRGLQFLDLIQEGNVG
                     LMKAVEKYDYRRGNRFSTYATWWIRQAVTRALANHGRTIRIPAHLGGRISKLYQVAQE
                     LEQEYGRQPTAEEIAENMELPADRVRWMLRTSRQPVHLERPVGDESDAELGDFIEDIE
                     APAPAESVAQTMLTEEIGDILDQLTPREARILRLRYGLQDGESRTLKEVGEMFGLSRE
                     RIRQLEKEALRKLRHPNFAGHLRQYLN"
     gene            1970..>3093
                     /locus_tag="PLC06_14830"
     CDS             1970..>3093
                     /locus_tag="PLC06_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRO91770.1"
                     /translation="MKPNRTLILMLILMLATALTACGGGGDKEPEATIAPIATQAQVA
                     PTEAPPTEAPAPTEEPAPSGPAAESSFQDAMKATIQIEAQGSFMDPEFGMQLNTAGRG
                     SGFIIDPSGIAVTNNHVVTGAAFLQVYVQGNDRPLNAKILGVSECNDLAVIDIEGDGF
                     PYLDWSSAPARLGTEIYALGFPLGDPEPTLTRGVISKERAGGETSWASVNNVVEHDAT
                     INPGNSGGPLVTSDGKVVGVNYAGNSGVNQYFSINASDARSVVEKLRAGSDLDSIGIN
                     GEAVITGDNTTGIWVASVKSGSPADKVGVQAGDIITKLEGLVLSTDGTMADYCDILRS
                     HNASDPMNIEVLRFATQEVLAGQLNGPKLEQTFSFAQAVEV"
BASE COUNT          670 a          978 c          822 g          623 t
ORIGIN      
        1 gtcatttccc ggaccaatcc ccttggccgc tacctgcgat gcgtgcgcat catgtcagac
       61 agaactatta tgcaacatct gttccgcccg acataccctg aataccttac cacatccggc
      121 ccgaatccgg ataaatggct tgtgaaaccg tggtgaacat cacgtaaaac caataaccga
      181 cgaagaccga aaataaaaac accctctcgc ggcgagaggg tgtttgctgt caacatttga
      241 acacctagtt caggtattgc cgcaagtggc cggcgaagtt gggatggcgc agcttgcgca
      301 gcgcttcctt ctccagctgg cggatgcgct cgcgtgagag gccaaacatc tcgccgactt
      361 ccttcagtgt gcgcgattcg ccatcctgca agccatagcg caggcgcagg atgcgcgcct
      421 cgcgtggcgt caactggtcc aggatgtcgc cgatctcctc ggtcagcatc gtctgggcca
      481 ccgattcggc cggggccggg gcctcgatgt cttcgatgaa atcgcccagc tcggcgtcgg
      541 actcgtcacc caccggccgc tccagatgga ccggctgacg gctggtgcgc aacatccagc
      601 gcacgcgatc ggccggcagc tccatgttct cggcgatctc ctcggccgtc ggctggcggc
      661 cgtattcctg ctccagctcc tgggccacct gatagagctt gctgattcgc ccacccagat
      721 gggccgggat acgaatcgtg cggccgtgat tggccagcgc acgggtgacc gcctggcgaa
      781 tccaccacgt cgcataggtg ctgaagcggt tgccgcggcg ataatcgtac ttctcgaccg
      841 ccttcataag gccaacattg ccttcctgga tcagatcgag gaactgcaag ccacggccgc
      901 gatatttctt ggcgatgctg acgaccagac gggtgttggc gcgaatcaaa tgcgcacgag
      961 cggcgtcgcc aacctcgcgc tggcgctcca gcagatcgcg cgcgtcaagg tcaagctcct
     1021 caaccgactc caggcgctcg acggccgccc ggccggcttc aatctccatg gccagatgga
     1081 cttcctcatc ggccgacagc aacgactgct ggcccatctc acggaaatag agacccacgg
     1141 agtcatcgat cggcacgttg ctcaaatcga acaatggcgc gtcatgggaa cgcacccgag
     1201 ccggattcac aacacgcgtt tcccgcgggg caggagcctc atcgtccaga tcggcttcaa
     1261 gatcctcatc atcgccatcg acgatcaaag ccgcggcgac aatatcttcc tcgttctcgt
     1321 aaatgacgag gccgagagtc tgggcttctt ccagcaatgt ctccagcagc gttatattat
     1381 tttcgatctc gggcaacgct tccagaatct gatcatacgt caggtatcgc tgctcgatcc
     1441 cttcattcaa aagcgcgttg aggatttcca tttcatccga caatggatcg ttcaggtctg
     1501 tcatatcaaa ataattcctt gcggtctccc tcggtcttca acaaggcccc gaagcttcgc
     1561 gatataattt cggggacccg agatgcgtgc aatcctgctg aatgccgtca aacagcgaga
     1621 cgggataatg ataaaataat gatgtctctg tgctgtcgat gggtcggctt tcgcctaatg
     1681 catctctacc tcttccttat agatcaaaag tgcagtctag cataagctgc agacaaaagt
     1741 caataggccc ggcggcttga tctcgccttt ttgacgcgcc gcgacatcga ttattcattc
     1801 gtatctcata gacgaaaaat gacgctaatt attaccaaaa tacatcgatt gcttaacttt
     1861 tcctaaggtc aagatcatgt ataatgtcat ccataatgag ggagatgagc cacttcgcct
     1921 tcatttatga gtgcagaata tcgttaatat tttatcttcg gaggtcggga tgaaacccaa
     1981 tcgaaccctt attctcatgc tcatactgat gctcgcaacc gcgctgacgg catgtggcgg
     2041 cggcggtgac aaagagcctg aagccaccat cgcaccaatc gccacacagg cccaggtcgc
     2101 gcctaccgag gctcccccga ccgaggcgcc cgcgcctact gaagagccgg ccccatccgg
     2161 tccggcggcc gaatcgagct tccaggacgc catgaaagcc acgatccaga tcgaggccca
     2221 gggcagtttc atggacccgg aattcggcat gcagctgaac accgccggtc gcggctccgg
     2281 tttcatcatc gacccgtcgg gtatcgccgt cacgaataac cacgtcgtca ccggcgcagc
     2341 cttccttcaa gtatacgtcc agggcaacga tcggccgctc aacgccaaga tcctcggtgt
     2401 gtcggaatgc aacgacctgg ccgtcatcga tatcgaaggt gatggcttcc cgtacctcga
     2461 ctggagcagt gccccggccc gcctcggcac cgagatttac gccctgggct tcccgctggg
     2521 cgacccggag ccgaccctga cccgcggcgt tatctccaaa gagcgagccg gcggcgagac
     2581 aagctgggcc tcggtgaaca acgtcgtcga acatgacgcc acaatcaacc ccggcaactc
     2641 cggtggccca ctggtaacgt ctgacggcaa ggtcgtcggc gtcaactacg ccggcaattc
     2701 cggcgttaac caatatttct ccatcaacgc ttccgacgcc cggtcggtcg tggaaaaatt
     2761 gcgtgccggc agcgatctcg attccatcgg catcaacggc gaagcggtca tcaccggcga
     2821 caacaccacg ggtatctggg tcgcctcagt gaagtccggc tccccggccg acaaggtcgg
     2881 cgtccaggcc ggtgacatca tcaccaagct ggaaggactc gtcctgtcca cggacggcac
     2941 catggccgac tattgcgaca tcctgcgcag tcacaacgcc agcgacccga tgaacatcga
     3001 ggtgttgcgc ttcgccactc aggaagtcct ggcagggcag ctcaatggcc ccaaactgga
     3061 acagaccttc tccttcgctc aggccgtaga ggt
//
LOCUS       DAOMQG010000381         3089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12808_length_3089_cov_11.803889, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000381 DAOMQG010000000
VERSION     DAOMQG010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12808_length_3089_cov_11.803889"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1053
                     /locus_tag="PLC06_14835"
     CDS             211..1053
                     /locus_tag="PLC06_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91771.1"
                     /translation="MKKPKIAILFGVLLVLALTASAFAELVVTYTSSDDMWNEITIED
                     TAEREAAAATNSLIDNGAFTKWGTTLGPADPWVFWRDTTAGWTAGRLHEVDLALPANP
                     DGINNAMGWFIQHSGAKEGGYYAGAYQQLTRIPAPGLYYVSISTTAFSDGKTGPYNSV
                     AWYSISDSANPAEVTDWRELFPDQFVCANGDGVCNYVGRDETVQINPGQYFHLKVGRK
                     FPEFLGWTMFVIDDISIVAADGTAGTENGFYNWCNGRPEDFKVHEAGCTSPTEIHWDP
                     EQTR"
     gene            1171..2490
                     /locus_tag="PLC06_14840"
     CDS             1171..2490
                     /locus_tag="PLC06_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRO91772.1"
                     /translation="MLIYRSVTASTKRGLLLAALCLLSLITLSQTACTRDEVVAAVST
                     IAVDLEATLQPWGATTVANVQMAVTQVPAMISTSKATRPEKQPDEQEQGVIRQIGQPI
                     EKPAQIAPTATNTIAATETSLPSPTASSTSTPTSTSTETSIPSPTATSTPTATPTPYP
                     MEIDVPGGSMLRIPGGTFYMGATAAGLAKECSAFRDGCQSDWFSASEPIHAVLLRSYY
                     IDAHEVTNAAYVEFLNQNGNDCLEQQCVDLAQSSLEQDGSTFAIDETQSQYPVTGATW
                     YGAQAFCTWRDGRLPTEAEWEKAAAWDDKTTTARQYPWGDVFDGRLVNSCDGSCDEPQ
                     ANEAFDDGFATLAPVASFPGGRSAFGLYDMAGNVWEWVSDWYDPDYYQVSAEANPAGA
                     KEGKDKVVRGGSWFDTGNFMATTIRFPSPPANADRTIGFRCAADLHN"
     gene            complement(2502..>3089)
                     /locus_tag="PLC06_14845"
     CDS             complement(2502..>3089)
                     /locus_tag="PLC06_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRO91773.1"
                     /translation="AAQIIRAGGVPEMMGIVPDDLGALREASREGLRRSDMLVMSAGS
                     SVSARDMTAKIIEELGQPGVLLHGVATRPGKPTIAGAVDGKPVLGLPGNPVSAMVQFD
                     MFGTPAIHRLLGLAETFRRGRFMAFLEQNIASETGREDYVPGRLVERDNSLIVVPIFG
                     KSNLINTLVQADCLIKIPLNKGGLMAGESVEILFP"
BASE COUNT          701 a          863 c          831 g          694 t
ORIGIN      
        1 ccattattaa catgggcttc acgcgtgagg cgctctatct gtggtacaaa gaatattgag
       61 taatcttgtt gcctgtctac cctgttccat ttgtacgtcc cgttccagta ttgggataag
      121 gaggatttac ttactgaaca ctcaaagagc gaacattaac ccgtatttga tcagtcgtat
      181 tgtcagtcaa ccataaggag ataccgagta atgaagaaac ccaaaatagc catactgttt
      241 ggagtgttgt tggtgcttgc cctgacggca agcgcatttg ccgaactcgt ggtgacgtat
      301 acttcgtccg acgatatgtg gaatgagatc accattgaag ataccgccga gcgcgaggct
      361 gcggccgcga ctaacagcct gatcgataat ggtgcattca ccaaatgggg aacgaccctc
      421 ggtccggcag acccgtgggt tttctggcgc gatacgacgg cgggctggac agccggccgc
      481 ctccatgaag tggacctggc gcttcccgcc aatcccgatg ggatcaacaa cgcgatgggt
      541 tggtttattc aacattccgg agccaaggaa ggcggctatt atgccggtgc ttaccagcag
      601 ctgacccgca tccccgctcc cggcctgtac tatgtcagta tcagcacgac cgccttctcc
      661 gacggaaaaa ccggcccata taacagcgtg gcctggtatt cgatcagcga ttcggctaac
      721 ccggccgaag tgacggattg gcgcgagtta ttcccggatc agtttgtctg cgcgaatggc
      781 gatggcgtat gcaattacgt cggccgcgac gagacggttc agatcaaccc gggtcaatat
      841 ttccatctga aggttgggcg caaattcccc gagtttctgg gctggacgat gtttgtcatt
      901 gacgacatca gcattgtagc cgcagacggc actgccggca cagaaaacgg tttctataac
      961 tggtgtaacg gccgtccgga agacttcaag gttcatgagg ccggctgcac aagcccgacc
     1021 gagatccatt gggatccgga acaaaccaga taagtaagta acgcagggtt tatggtataa
     1081 agagcgcgag tcggcctgat catgcttgcc gactcgcgct tgtcgtttat tgggcggatc
     1141 cgtctcgttt cgttcggtta gggaagtcca atgttaatct atcgatcggt tactgcctcc
     1201 acaaagaggg gcttgttact ggccgcactt tgcctgctgt cattgatcac tctcagccaa
     1261 actgcttgca cgcgcgatga ggtcgtggcc gccgtttcga cgattgcggt ggatctcgag
     1321 gccaccttgc agccatgggg ggcgacgacg gtcgccaacg tccaaatggc tgtgacccag
     1381 gttccagcga tgatttccac ctcaaaagcg accagacctg agaagcagcc cgatgaacaa
     1441 gagcagggag tgattaggca gattggtcag ccgattgaaa agcctgccca gatcgcacca
     1501 accgctacta ataccatagc cgcgacagaa acttcacttc cttcacctac ggcttcatct
     1561 acatccacgc ccacgtcaac atcgacggag acgtccatcc cttcaccgac cgcaacgtct
     1621 acaccgacag caacgccaac tccctatcca atggaaattg atgttcctgg cggatcaatg
     1681 ctccgaatcc cgggtgggac attctatatg ggcgcaacgg ccgccggcct cgccaaagag
     1741 tgttccgcgt ttcgtgacgg ctgccaaagt gattggtttt ccgcctccga gccgattcac
     1801 gcggttttgt tgcgcagtta ttatattgat gcccatgagg tgaccaatgc cgcttatgtg
     1861 gagttcctca atcagaatgg taacgattgt ctggagcaac aatgcgtgga cctggcgcaa
     1921 agcagtctcg aacaagatgg aagcactttt gctatcgatg aaacccaatc acaatatccc
     1981 gtaactggcg ccacgtggta tggcgcgcag gcgttctgca cctggcggga cggccgcttg
     2041 cccacagaag cggaatggga aaaagcggcc gcatgggacg acaaaaccac aacggcgcgg
     2101 cagtatccgt ggggagatgt attcgacggc cgacttgtca attcatgtga cggttcttgc
     2161 gatgagccgc aggcgaatga ggcctttgat gatggctttg cgactcttgc ccctgtcgct
     2221 tcatttccag gcgggcgaag cgcatttggc ctgtatgata tggctggcaa tgtttgggaa
     2281 tgggtgtccg attggtatga tcctgactat tatcaagtgt cggccgaggc caacccggcc
     2341 ggggcaaagg aagggaagga taaagtcgtg cgcggcggtt catggttcga tacgggcaat
     2401 ttcatggcga cgacgattcg cttcccttca cctcccgcaa acgccgacag gacgataggg
     2461 ttccgttgcg ccgccgattt gcacaactaa tctctgatag atcaagggaa taaaatctct
     2521 actgattcgc ccgccatcag cccgcccttg ttcaacggaa ttttgatcag gcaatcggcc
     2581 tgtacgaggg tattgattag gttactcttc ccgaatattg gtaccacgat gagcgaatta
     2641 tctcgctcaa cgaggcgccc tggaacataa tcttcgcgcc ccgtctcgct ggcgatattc
     2701 tgctccagaa aagccataaa tcggcctcgc ctgaacgttt ccgccaggcc gagcagccga
     2761 tggattgccg gggttccgaa catatcaaac tgaaccatcg ctgagaccgg gttgcccggc
     2821 aatcctaaaa cgggcttgcc gtccaccgca ccggctattg ttggttttcc cggccgtgtg
     2881 gcaacgccat gtaatagcac acccggctgc cctaactctt caatgatctt tgccgtcata
     2941 tcacgcgcgc tgaccgaaga cccggccgac ataaccagca tatcgcttct tctcaaccct
     3001 tcccttgagg cctcgcgcaa ggcgcccagg tcgtcgggca caatccccat catttcaggc
     3061 acgccaccgg ccctaatgat ctgtgcggc
//
LOCUS       DAOMQG010000382         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12888_length_3075_cov_8.375497, whole genome shotgun sequence.
ACCESSION   DAOMQG010000382 DAOMQG010000000
VERSION     DAOMQG010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12888_length_3075_cov_8.375497"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLC06_14850"
     CDS             complement(<1..680)
                     /locus_tag="PLC06_14850"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HRO91774.1"
                     /translation="MVIAMFLAHLVGDYVLQWDSLAQWKAREFRGVAVHSLILFLVTF
                     AFALPFPPMWWSGVLIIGGSHFLIDSLQFFHKAQIPPLLRFSLDQLTHMAIIILALVA
                     GGYLEWGHIWSGILQSANETPLLTTATAYAFITMPAWVLLKYIAYAAAKDKAPNFLAG
                     PNKFIGIAERLIIVTLVMFGQFLLVPLVALPHVVLAWPQLTQGQSKAAYAIELVAGVV
                     LAVGVGVAL"
     gene            complement(985..>3075)
                     /locus_tag="PLC06_14855"
     CDS             complement(985..>3075)
                     /locus_tag="PLC06_14855"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRO91775.1"
                     /translation="YTDMSVVIWALRLQIILAVLTGLALLYNVFRPNLRLPLIAGGLW
                     VLVAVVGGGLLPGIIQRYVVEPNELTLETPYIEYNIELTRFAYGLDRVETREFGQVED
                     LQPRDLLENEDVLSNIRLWDWRPLEQTYRQLQGLRPYYDFSDVDIDRYMIDGKQRQVM
                     LGTRELDKRKLPAPSWVNRSLEFTHGFGIVMNPVNEVTRDGQPEFFIKDFPPESNTSV
                     KVTRPEIYYGELTTDTVYVGSNRQEFDYPSGDSNIYTNYSGEGGVVLDTWLKRLAFVL
                     REGDPNILLSNDITSTTRAQFRRQVQSRIREITPFLILDGDPYMVVDDAGRLIWMQDA
                     YTASDAFPYATPMQITEKPGTTAAEGQYERSHTINYIRNSAKITLDAYNGTVNYYLSD
                     PSDPIINSYARAFPGVFQPMSAMPADLQAHVRYPEDLFTIQSRQYITYHMTDVRVFYN
                     KEDLWQIPTEISNTGETTAEPYYVILPLPGSDESEYLLILPFSPATRNNMIGWMAARN
                     DPAHYGQLIVYELPKQELIFGPIQIEGRINQEPIISQQFSLWDQRGSNVIRGNLLVLP
                     INRSFLYIEPVYLLSETSALPELKRIVTAGNTSIAMAETLRGSLLALAQGTTTTAPLT
                     PDEPPGVNEPPAGGAGATPVPETLEQLVAAANAHMQAAEAAQRDGDWATYGRELDALR
                     AVLADLSQLTGQDE"
BASE COUNT          590 a          874 c          999 g          612 t
ORIGIN      
        1 agggctaccc ccacgccgac agccagcacg acgccggcga ccagttcgat ggcataagcc
       61 gccttgcttt gaccctgggt cagctgcggc cacgccagaa cgacgtgggg cagagccacc
      121 agcggcacca gcaggaattg gccgaacatg accagcgtga cgatgatcag ccgctcggcg
      181 atgccgatga acttgttcgg cccggccagg aaattggggg ccttgtcctt ggccgccgcg
      241 taagcaatat acttgagcag cacccaggcg ggcatggtga tgaacgcgta ggccgtggcc
      301 gtcgtcagca gcggcgtctc gttggcgctt tgcaggattc cgctccagat gtgcccccat
      361 tccagatagc cgccggccac aagcgccagg atgatgatgg ccatatgggt caattggtcg
      421 agggagaagc gcagcagcgg ggggatttgg gccttgtgga aaaattgcag gctgtcgatg
      481 aggaagtggc tgccgccgat aatcaggacg ccgctccacc acatgggggg aaacgggagc
      541 gcgaaggcaa acgtcaccag aaacaggatt aaactgtgga cggccacgcc gcgaaattcg
      601 cgggccttcc attgggccaa tgaatcccac tgtaatacat aatcgcccac cagatgggcg
      661 aggaacatcg ctattaccat atctctaacg ggttatcgct acaccagatt tcttaagctt
      721 gtagtttgca gctttagctg cgttccccga gcggcgctga agcgcctgac tacgaacgaa
      781 cgatcattta gtgaaatatg cactgaagat ttcttaagct tgtagtttgc agctttagct
      841 acgttccccg agcggcgctg aagcgcctga ctacgaacga acgatcatat agtgaaatat
      901 acactgatga tcatataaca atatttcccc gcgtgctgta acttacgtta cacgaatcgt
      961 gttgagtcag gtttcgggaa tagcctattc gtcctgcccc gtcagctgag acaggtcggc
     1021 gaggacggcc cgcagggcgt cgagttcgcg gccgtatgtg gcccaatccc catcgcgctg
     1081 cgcggcctcg gccgcctgca tgtgggcatt ggccgccgcc accagctgct ccaatgtctc
     1141 cgggacgggc gtcgcgccgg cgcctccggc cggcggttcg ttgacgcccg gtggttcgtc
     1201 tggcgtcaat ggcgcggtcg tcgtcgttcc ctgggccagc gccagcagcg atcccctcaa
     1261 cgtctcggcc atggcgatcg aggtgttgcc ggcggtgacg atgcgtttca gttccggcag
     1321 ggcgctcgtc tccgagagca gatagaccgg ctcgatatag aggaagctgc gattgatggg
     1381 cagcaccagc agattgccgc gaatgacgtt ggagccgcgc tgatcccaca gcgagaactg
     1441 ctgcgagatg atcggctcct gattgatgcg cccctcgatc tggatggggc cgaagatcaa
     1501 ctcctgcttg ggcaactcat agacgatgag ctgcccgtaa tgggccgggt cgttgcgggc
     1561 cgccatccag ccgatcatgt tgttgcgcgt ggccgggctg aagggcagga tgagcaggta
     1621 ttccgattcg tcggagccgg gcaggggcag gatgacgtag tacggctcgg cggtcgtttc
     1681 gcccgtgttc gatatctccg tgggaatctg ccacaaatct tccttgttgt agaagacgcg
     1741 cacatcggtc atgtgatagg tgatgtactg gcgcgactgg atggtgaaca ggtcttcggg
     1801 atagcggacg tgggcttgca ggtcggcggg catcgcgctc atcggctgga aaacgccggg
     1861 gaacgcgcgg gcatagctgt tgatgatggg gtcggacgga tcgctcaaat agtagttgac
     1921 cgtgccgttg taggcgtcga gcgttatctt ggccgaattg cgaatgtagt tgatggtatg
     1981 gctgcgctca tactgaccct cggcggccgt ggtgccaggc ttctcggtga tctgcatggg
     2041 cgtggcatag gggaaggcgt cgctggcggt gtaggcgtcc tgcatccaga tcagccgtcc
     2101 cgcgtcgtcg acgaccatgt aggggtcgcc gtcgaggatc aggaatggag tgatctcccg
     2161 aatgcgcgac tgaacctgcc gccgaaactg ggcgcgggtc gtggatgtga tgtcgttgga
     2221 cagcaggatg ttgggatcgc cttcgcgcag gacaaaggcc agtcgcttca gccaggtatc
     2281 gaggacgacg ccgccctcgc cgctgtagtt cgtgtagatg tttgaatcgc cgctcgggta
     2341 atcgaactcc tggcgattgc tgcccacata caccgtgtcg gtggtcagtt cgccgtagta
     2401 aatctcgggg cgcgtcactt tgacggaagt attgctctcg ggcgggaagt ccttgatgaa
     2461 aaattcgggc tgcccgtcgc gggtcacctc gttgacgggg ttcatgacga tgccgaagcc
     2521 gtgggtgaac tccagcgagc ggttgaccca cgacggggcg ggcagcttgc gcttgtccag
     2581 ttcgcgcgtg ccgagcatca cctggcgctg cttgccgtcg atcatatagc gatcgatgtc
     2641 gacgtcgctg aagtcgtagt acggccgcag cccctgcagc tggcgatagg tttgctccag
     2701 cggccgccag tcccacaggc ggatattgct cagcacgtcc tcgttttcca gcaggtcgcg
     2761 cggctgcaaa tcctcgacct ggccgaactc gcgggtctcc acccgatcca gcccataggc
     2821 gaagcgggtc agctcgatat tgtattcgat gtagggagtc tccagcgtca gctcgttcgg
     2881 ctcgacgacg tagcgctgga tgatgccggg cagcagcccg ccgcccacga ccgccaccag
     2941 cacccacagg ccgccggcga tgagcggcag ccgcagattg ggccgaaaga cgttgtagag
     3001 cagcgccagc ccggtgagga cggcgagaat gatctgcaag cgcaaggccc agatgaccac
     3061 gctcatgtcg gtgta
//
LOCUS       DAOMQG010000383         3067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12938_length_3067_cov_8.575365, whole genome shotgun sequence.
ACCESSION   DAOMQG010000383 DAOMQG010000000
VERSION     DAOMQG010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12938_length_3067_cov_8.575365"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLC06_14860"
     CDS             complement(<1..560)
                     /locus_tag="PLC06_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO91776.1"
                     /translation="MSTQSQPAIRLRNVSKRFAFTPDMPQSVLETAIAAVTRRRRRRD
                     RSHDLWAVHDVSFDVFPGQSLGIIGRNGSGKSTLLKIIARIIQPTSGEITVNGRVSAL
                     LELGAGFHPDLTGRENIYLNASVLGLSREETATLFNDIVEFSELDEFINMPVKHYSSG
                     MYMRLGFSVAIHVHPDILIVDEILAVG"
     gene            705..>3067
                     /locus_tag="PLC06_14865"
     CDS             705..>3067
                     /locus_tag="PLC06_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRO91777.1"
                     /translation="MFDQKSLTTLEFNKIRDIVAGYANFSGGRDLALAMQPTAELEQA
                     REWQAETREAVSLFETDSGVTIGGARDVRRAADNAMRGFILLPEDLLAIQATIVAGRN
                     LRRQLLRMEERVPHLAAVAVLIEECRGIVDAITNTIDERGDVLDSASPRLGKIRQEQR
                     AVHGRIQDKLQRILNSSMSQFLQEAIITQRGGRYVIPVRADAKGRLKGIVHDQSGSGA
                     TLWVEPLGTVDLNNEYRGLLMEEQEEIQRILRELSNLVADQGDAIQRVVERVAELDLI
                     FARANYALMTNAVEPTFVPWRETPAQRPPRHANQRDKLPELPRNMHPGSTVWIKAARH
                     PLIDSRAVVPTNLTLDEETFIVLITGPNTGGKTVSLKTMGLMALMAQSGLHLPANEAR
                     LTVFDNVFADIGDEQSIEQSLSTFSSHMTNIVRILDRVDERSLVLFDELGSGTDPTEG
                     AALAQAVVNFLRDKGATTFVATHYPELKIYAGQTPGATNASLLFNMETLSPTYEMTIG
                     LPGRSNAFAIARRLGLDGTILDDAMRQVSADSHQAEDLLDSIYTLREKMEAEEAQTRR
                     ALRDAEAERERLQRRLEEVEVERGRIVEETRQEMKRQIELLQTEIRQARSKLRDATSL
                     NAVKKLGKQATDLEVTPETILAPEPSEAAEVSRGKRRSLQVGDIVQVRSLNVKGEVIS
                     IGKNEVVVAVGRLQMRAGFDDLEFKERPVEEREPAAIGGLHPSPGMELDIRGVRVEDG
                     VEQLQRYIDSAFLARLPFVRIIHGKGTGRLRQAVREALSASKQVRAW"
BASE COUNT          595 a          871 c         1064 g          537 t
ORIGIN      
        1 cccacggcca gaatctcgtc gacgatgagg atgtcggggt ggacgtggat ggcgacgctg
       61 aagcccaggc gcatgtacat gccggaggag taatgcttga ccggcatatt gatgaactca
      121 tccagctcgg aaaactcgac gatgtcgttg aacagggtgg ccgtttcttc cctggagagg
      181 cccagcacgg aggcgttgag gtagatgttc tcgcggccgg tcaggtcggg gtggaagccg
      241 gcgcccagct ccagcaacgc gctgacgcga ccattgaccg tgatttcgcc ggaggtgggc
      301 tggatgatgc gggcgatgat cttcagtagg gtgcttttgc cgctgccgtt gcggccgatg
      361 atgccgagac tttggccggg aaaaacgtca aaagaaacgt cgtggacggc ccacaggtcg
      421 tgggaacgat cgcgtcgtcg ccgacggcgg gtgacggcgg cgatggcggt ttccagcacg
      481 gattgaggca tatccggcgt aaacgcaaag cgtttgctga catttcgcaa ccgaatcgcc
      541 ggttggcttt gcgtactcat aggcgcgcgg atagtatagc atgttgggta aattggcaac
      601 cgacgagcga gtgacgaatg gagaaaagta cgaagtacga acgacgaagt acgaaagtcg
      661 cgctcttcat ttgtcattcg tcattcgtca ttcgtaatta aattatgttc gatcaaaagt
      721 cacttacaac gctggaattc aacaaaatcc gtgacatcgt cgccggatat gccaacttct
      781 cgggtgggcg cgatctggcg ctggccatgc agccgacggc cgaattggag caagcccgcg
      841 aatggcaggc cgaaacgcgc gaagccgtct cgctgttcga gaccgacagc ggcgtgacca
      901 tcggcggggc acgcgatgtg cgccgggcgg ccgataacgc catgcgcggc ttcatcctgc
      961 tgccggagga tctcctggca attcaggcga ctatcgtcgc cgggcgcaat ctgcgtcgcc
     1021 agctgctgcg aatggaggag cgcgtgccgc atctggcggc cgtcgccgtc ctcatcgagg
     1081 agtgtcgcgg catcgtcgac gctatcacca acaccatcga cgagcgcggc gacgtgctcg
     1141 acagcgccag cccgcgtttg ggcaaaattc ggcaggagca gcgcgccgtc catggccgca
     1201 ttcaggacaa gttgcaacgc atcctcaata gcagcatgtc ccagtttttg caggaagcga
     1261 tcatcaccca gcgcggcggc cgctatgtca tccccgttcg cgccgacgcc aaggggcgac
     1321 tgaagggcat cgtccacgac cagagtggca gcggggccac gttatgggtg gagccgctgg
     1381 gcacggtgga tctcaacaac gaatatcgcg gcctgctgat ggaagagcag gaggagattc
     1441 agcgaatcct gcgggagctg tccaatctgg tggccgacca gggcgacgcc atccagcgcg
     1501 tcgtggaacg tgtggccgag ctggacctga tcttcgctcg cgccaattac gcgttgatga
     1561 ccaacgccgt ggagccgacg ttcgtgccgt ggcgggaaac gccggcgcaa cggccgccgc
     1621 gccatgccaa ccagcgcgac aagttgcccg aactgccgcg caatatgcac cccggctcga
     1681 ccgtctggat caaggctgcc cgccatccgc tcatcgactc gcgcgccgtt gtgccgacca
     1741 atctgacgct ggatgaggag acgttcatcg tcctcattac tgggccgaat accggcggca
     1801 agacggtgag cctgaagacg atgggcttga tggcgctgat ggcccagagc gggctgcacc
     1861 ttccggccaa cgaggcgcgg ctgaccgttt tcgataatgt gtttgccgac atcggcgacg
     1921 agcagtcgat tgaacaaagc ctgtccacgt tctcgtcgca tatgaccaat atcgtgcgta
     1981 tcctggacag ggtggacgag cgctcgctgg tgctgttcga cgagctgggt tccggcaccg
     2041 acccgacgga aggggcggcg ctggcccagg cggtggtcaa cttcctgcgc gacaaggggg
     2101 cgacgacctt cgtggccacc cattacccgg agctaaagat ctatgccggg caaacgccgg
     2161 gggccaccaa tgcttcgctg ctgttcaata tggagacgct gtcgccgacc tatgagatga
     2221 ccatcggcct gcccggtcgt tccaacgcct tcgccattgc ccggcggctg gggctggacg
     2281 ggacgatcct cgatgacgcc atgcgccagg tgagcgccga cagccatcag gccgaggatt
     2341 tgctcgattc gatctatacc ctgcgcgaga agatggaggc ggaggaggcg cagacgcggc
     2401 gggccttacg cgatgcggag gccgagcgcg agcggctgca gcggcggctg gaagaggtgg
     2461 aggtagagcg cggccgcatt gtggaggaga cgcgccagga gatgaagcgg cagatcgagt
     2521 tgttgcagac ggagattcgc caggcgcgct cgaagttgcg cgacgcgact tcgctcaacg
     2581 cggtcaagaa gctgggcaag caggcgaccg atctggaggt gacgccggag acgattctgg
     2641 cgccggagcc gagcgaggcg gccgaggtgt cgcgcggcaa gcggcgctcg ctgcaggtgg
     2701 gggatatcgt ccaggtgcgc tcgctcaatg tgaagggcga ggtcatctcc atcggcaaga
     2761 atgaggtggt cgtggccgtc ggccgcttgc agatgcgggc cgggttcgat gatctggagt
     2821 tcaaggaacg gccggttgag gagcgggagc cggcggcaat cggcgggctg catccgtcgc
     2881 ccggcatgga actggacatt cgcggcgtgc gggtggagga tggcgtggag cagttgcagc
     2941 gctatatcga ttcggctttt ctggcgcggc tgccctttgt gcgcatcatc catggcaagg
     3001 ggacggggcg attgcggcag gcggtgcggg aggcgctgag cgccagcaag caggttcgcg
     3061 cctggga
//
LOCUS       DAOMQG010000384         3065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12943_length_3065_cov_11.699336, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000384 DAOMQG010000000
VERSION     DAOMQG010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12943_length_3065_cov_11.699336"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1140)
                     /locus_tag="PLC06_14870"
     CDS             complement(250..1140)
                     /locus_tag="PLC06_14870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRO91778.1"
                     /translation="MPWGELAALGTAVCWSFTAIFFSISGRRIGSDVVNRSRLLFAFG
                     FLLITHWFLEGVLFPSGVEPFRWGWLAISSILGLVLGDAFLFQAYVIVGPRLSMLMMS
                     TVPIISTLFGLLLFGETVTSLEMMGVALAVVGVAWVVTEGQSSASTMDRRTYNKGLLF
                     GLLGALGQVANLVTARYALVDDFPAISATLIRILIAIVVLWGIAAVRGQIGHTFRQWK
                     DKKAFQAMMGGAVVGPFLGIWLSLIAVQHARLGIASTLMALPPVLLIPLEYLIYRHRV
                     SRRGIAGTVIAMVGVALLFV"
     gene            complement(1168..1656)
                     /locus_tag="PLC06_14875"
     CDS             complement(1168..1656)
                     /locus_tag="PLC06_14875"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRO91779.1"
                     /translation="MAAKLSHKEYQQALPRKRMAAGCLFFDADERLLVVNPTYKDGWE
                     IPGGVVETNESPLAACIREIREELGIDRQPRGLLCVDYMAETSQRTEALNFIFDGGVL
                     PDDVIAAIRLPAKELAEYRFFPSDQALALMKPRLRNRVACCLNSRDAGMTIYLEEEVP
                     IW"
     gene            complement(1641..2606)
                     /gene="mmuM"
                     /locus_tag="PLC06_14880"
     CDS             complement(1641..2606)
                     /gene="mmuM"
                     /locus_tag="PLC06_14880"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HRO91780.1"
                     /translation="MSDKANPILPFLQKQGALILDGGLATELERQGYDLGDALWSARL
                     LVDAPDAIRQVHLDYYWAGADCCTSASYQATIPGFVGRGMTPETARDLIRLSVQLVIE
                     ARDAFWALEQNHVRRLRPIVAASVGPYGAYLADGSEYTGDYDLDETELTDFHCERWGI
                     LASAGADILACETIPSFVEARALVALLDATPGAWAWFSFSCRDGEHISDGTPIAECAA
                     FLAEHERVAAIGINCTPPRFIPDLIRAVSAATDKPIIVYPNSGETYDPVAKRWLGESV
                     PSEFGTYSREWRKLGAGLIGGCCRTTPAHIRQISDRFRRRKWPLN"
BASE COUNT          725 a          931 c          826 g          583 t
ORIGIN      
        1 atttatggcg atgccgtcaa cgggagcgct accagcgaaa cgctgagtca gtggctcgcc
       61 gcgggcggcg ccggcctgtg ccaacaagcg gccgcacggc tggggttgtg acctggtcgg
      121 actccttcgc catcacacca tcccaaaggc agccggtcgc aatgactgac tgcctttttt
      181 cttttataca tcccagcacg cccgcagtct cattcaggtt attcacgcca catccccgaa
      241 aacctgatat caaacgaaca ataacgctac ccccaccatg gcgatcacgg taccggcaat
      301 tcctcgacgg ctgacccgat ggcgatagat cagatactca agcgggatga gcaaaaccgg
      361 cggcaaggcc atcaaggtcg aggcgatccc cagtcgggcg tgctgaacag cgattagcga
      421 cagccaaatc cccaggaacg ggccgaccac tgccccaccc atcatcgcct gaaaagcctt
      481 cttatccttc cattggcgaa aagtgtggcc gatctgcccg cgaacggcag cgatccccca
      541 caggaccacg atcgcgataa ggatgcggat caacgtggcg gagatggccg ggaaatcgtc
      601 caccagcgca taacgggctg taaccagatt ggccacctgg cccaatgcgc cgagtaatcc
      661 gaagagcaga cccttattgt aagtacgtcg atccatggtc gacgcgctgc tctgcccttc
      721 cgtgaccacc catgctacac cgaccaccgc caacgccacc cccatcattt ccagcgacgt
      781 gaccgtttcc ccaaagagca gcagaccgaa caaagtactg atgatcggca cggtcgacat
      841 catcagcatc gacaagcgag gcccaactat cacataagcc tgaaacagga aggcatcgcc
      901 caataccagg ccgagaatac tggaaatcgc cagccagccc catcgaaacg gctcgacgcc
      961 ggagggaaac aggacgccct ccagaaacca gtgggtgatg agcaggaacc cgaaagcaaa
     1021 cagcaatcgg ctgcgattga cgacatcgga accgatgcgc cggccgctga tgctgaaaaa
     1081 gatagcggtg aacgaccagc agacggccgt ccccaaagcg gcgagttctc cccagggcat
     1141 aagtttatta tccttttgtg gcgcggatta ccagatcggc acttcttctt ccagatagat
     1201 cgtcatgccg gcatcacgcg agtttaggca acaggcaacg cgattacgca gtcgtggttt
     1261 cattaaggcc agcgcttggt ctgacgggaa aaagcgatat tcggccagct ctttcgcggg
     1321 caagcgaatg gcagcaatga cgtcatcggg gaggaccccg ccatcaaaaa tgaaattcag
     1381 ggcctccgtt cgctgtgacg tctcggccat ataatcgacg caaagcaatc cgcgcggttg
     1441 ccgatcgatc cctaactctt cccgtatttc gcgaatgcag gcggccaaag gggattcatt
     1501 cgtttccacc acgccgcccg gtatttccca gccatccttg taggtggggt tgacgaccaa
     1561 taatcgttcg tccgcgtcga aaaacaggca gcccgcagcc atacgctttc ggggtagcgc
     1621 ctgctgatat tccttatgac ttaatttagc ggccattttc tccttcgaaa tcgatcgctg
     1681 atttgcctga tatgggccgg cgtcgtgcgg caacagccgc cgattagccc cgcgcccagc
     1741 ttgcgccact cgcggctata ggtgccgaac tccgacggca cggactcccc cagccagcgt
     1801 ttggccaccg gatcataagt ctcgcccgag ttggggtaga cgatgatcgg cttatccgta
     1861 gcggccgaca cggcgcggat gagatcgggg atgaaacgcg gcggcgtgca gttgatgccg
     1921 atagcggcga cgcgctcatg ctccgccagg aaggcggcac attcggcaat gggcgtgccg
     1981 tcgctgatat gttccccatc gcgacaactg aagctgaacc aggcccaggc tcccggcgta
     2041 gcgtccaaca gcgcgacgag cgcgcgcgct tcgacaaacg acgggatcgt ctcgcaagcc
     2101 agaatgtcgg ctccggccga ggccaatatg ccccatcttt cgcagtggaa atccgtcaat
     2161 tctgtctcgt ccagatcata atcgccggtg tactccgacc cgtcggccag atacgcgcca
     2221 taagggccga cgctcgcggc tacaatcggc ctaagccgtc tgacatggtt ttgctccaat
     2281 gcccagaacg catcccgcgc ctcgatcacc aattgcaccg agagccgaat gagatcgcgg
     2341 gcagtctcgg gcgtcatgcc gcggccgaca aagccgggga tggtagcctg gtaactggcg
     2401 ctggtgcagc aatcggcccc ggcccagtaa taatccaggt gaacctggcg gattgcatca
     2461 ggcgcgtcaa ccagcaagcg agcggaccat aaggcatcac ccaagtcata tccctggcgc
     2521 tccaattcgg tcgccagccc gccgtcgagg ataagcgcgc cctgcttttg caggaacggc
     2581 aagattgggt tcgccttgtc gctcatgggg gtgttcttgt cgaaggagga gatgatccat
     2641 tcaatttctg aactgccgga tcaattatgt gacctaacag gatgatcggc aaatcatttc
     2701 gccctgcaaa taaaacaagg tcattatcgg ccctattcac cgatagacta tgcgcaaagc
     2761 cttctgctct ctttcgctaa ttccacgcct tatcccgaaa gcatgatccg attgtaagat
     2821 attcattgga aaggccgccc gcctattgac tctcccggac gattgttgat aatcacagca
     2881 aggagaaata cagactccta aggaacaact gtgatgatag attttttgat cacatattgg
     2941 ttagctgttg tgccgactgc cgtcaccgga gttctcttgc tgggcctgct gaagaagatg
     3001 cgcgacgaaa aagccaggaa ggaaaagggg cttgcacctg tcgccgctca gagaaaatat
     3061 caaga
//
LOCUS       DAOMQG010000385         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_12999_length_3055_cov_4.655000, whole genome shotgun sequence.
ACCESSION   DAOMQG010000385 DAOMQG010000000
VERSION     DAOMQG010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12999_length_3055_cov_4.655000"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..686
                     /locus_tag="PLC06_14885"
     CDS             189..686
                     /locus_tag="PLC06_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91781.1"
                     /translation="MHHRGFGFGRFILFLLLIGGLIAFAGGRYRSGFQDGFVRGMAFT
                     AVDGASGASAATPAVPPAYAGPWASGWGHGGSGFVGGGFPLGFGLLGFAFIAFMAMLF
                     MGAMGRRCGPRHEWAGHGPHGPRSPHDRHGRHGRHGPYSPWDHHRGERPSDEVGPEKQ
                     PEDIA"
     gene            754..1434
                     /locus_tag="PLC06_14890"
     CDS             754..1434
                     /locus_tag="PLC06_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91782.1"
                     /translation="MPDLILIIEDEPRIAKVARDYLEKYGYRTQIAADGHTGLAAARR
                     DSPDLVLLDVLLPGMDGREVCRRLRAESAVPIIMLTALAEESDQIVGLELGADDYIIK
                     PFSPNVLVARVRAALRRARGDPAATVPLRVGDLEIDPVAHTAMQSGRPVHLTPNEFRL
                     LAALARHAGRTLSRDQLLDELHGAALGGIDRSVDSHIKNLRRKLERDPERPLIETVYG
                     VGYRLVEQ"
     gene            1458..2585
                     /locus_tag="PLC06_14895"
     CDS             1458..2585
                     /locus_tag="PLC06_14895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRO91783.1"
                     /translation="MKPHPNPPKEKRPWPPPEWSGQSWSPEWEQRRRAFMRRFIAVFL
                     FASAIIALLFIGVGVLIAQLFRQRPIGSAESLFLMACTGGIVLLGVIFIGGLMARRFG
                     SPLARLMAAADAVAAGDLSVRVPVRGGGNMARFIERFNRMIAELERAEQSRRNLTADV
                     AHELRTPLHILQGNIEGVVDGVYEPTADHMTAMLDETRLLARLVDDLQTLSLAEAGQL
                     PLHRQTLPAADLLADVATRFAAAAADAGVALDLLPGGDAINVEVDPDRLDQALSNLVA
                     NALRHTPAGGRATLSAERAAGGVELVVADTGAGIEAADLPYIFDRFWRGDRARGRGGS
                     GLGLPIARRLIEAHGGTITVDSAVGEGTRFVVWLKEATSDE"
     gene            2665..2790
                     /locus_tag="PLC06_14900"
     CDS             2665..2790
                     /locus_tag="PLC06_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91784.1"
                     /translation="MVPETHSFQAPAFYQALGFRIVGTVENYPVGQQYYMPAKQL"
BASE COUNT          506 a         1036 c          965 g          548 t
ORIGIN      
        1 cccactgacc cactgaccca ctgacccact gacccactga cccactgacc cactcgtctt
       61 cattcataat tccaaatttc ccccatcatt tctccacaat tgcctgttac cttatccctg
      121 gcaaacgaca acaagagccg ggcgggttcc atggaacctg aacggactca cacaatcgga
      181 ggtaacacat gcatcacaga ggatttggtt tcgggcggtt tatcctgttt ctgctgctta
      241 tcggcgggct gatcgccttc gccggcgggc gctatcgctc cggcttccag gacggatttg
      301 tgcgcgggat ggctttcacc gccgtcgatg gtgcttccgg cgcttcggcc gccacgcccg
      361 ccgttccccc ggcctatgcc ggtccctggg cgtcggggtg gggccatggc ggctccggct
      421 ttgttggggg cggttttccg ctgggcttcg gcctgctggg ctttgccttc atcgccttca
      481 tggccatgct gttcatgggc gcgatggggc gacgctgtgg cccacgccac gagtgggcgg
      541 gccacggacc ccatggcccc cgcagccccc acgaccgcca cggccgccac ggccgccacg
      601 gcccctacag tccgtgggac caccaccggg gcgagcggcc gtcggatgag gtcggcccgg
      661 agaagcaacc ggaagacatc gcctaatagg gcaagacctg acaggttttc cgaaacctgt
      721 caggtctcaa tcatttccca tatacttacc cccatgcccg atctcatcct gatcatcgaa
      781 gacgaacccc gcatcgccaa agtggcccgc gactatctgg agaagtacgg ctaccggacg
      841 cagatcgccg ccgatggcca caccgggctg gccgccgccc gccgcgactc acccgacctg
      901 gtgctgctcg atgtgctgct gccgggcatg gacggccgcg aagtctgtcg ccgcttgcgg
      961 gccgaatcgg ccgtacccat catcatgctc accgcgctgg ccgaggagag cgatcagatc
     1021 gtcggcctcg aactgggggc cgacgactac atcatcaagc cattctcgcc caacgtgttg
     1081 gtggctcgcg tgcgagccgc cctgcgccgg gcgcgcggtg atccggccgc caccgtcccc
     1141 ctgcgcgtcg gcgatttgga gatcgacccc gtcgcccaca ccgccatgca aagcggccgc
     1201 cccgtccacc tgacgcccaa cgagttcagg ctgctggccg ccctggcccg ccacgccggc
     1261 cgaacgctga gccgcgatca actgctggac gaactccacg gcgcagcgct gggcggcatt
     1321 gaccgcagcg tcgattccca catcaagaac ctgcgccgca agctggagcg cgaccccgaa
     1381 cgaccgctca tcgagacggt ctacggcgtg ggctatcggc ttgtcgaaca atagcgctcc
     1441 aatcgcacag aatcgccatg aaaccacacc ccaacccgcc caaagagaag cgcccctggc
     1501 cgccgccaga gtggtcgggc caatcctggt cgcccgaatg ggagcagcgc cgccgcgcct
     1561 tcatgcgccg cttcattgct gtctttctct tcgcgtcggc catcatcgct ctgctgttca
     1621 tcggcgtcgg cgtgctgatc gcgcagctct tccggcagcg gccaatcggc tcggccgagt
     1681 cgctattcct catggcctgt acgggcggga tcgtgttgct gggcgtgatc ttcatcggcg
     1741 ggctgatggc gcgccgcttt ggctctccgc tggcccggct catggcggcc gccgatgccg
     1801 tggccgcggg cgatctttcg gtgcgtgtgc cggtgcgggg cgggggtaac atggcccgct
     1861 tcatcgagcg tttcaatcgt atgatcgccg agctggagcg cgccgagcag agtcgccgca
     1921 acctgaccgc cgacgtggcg catgagttgc gcacgccgct ccatatcctg cagggcaaca
     1981 tcgagggcgt ggtcgatggc gtctatgagc cgacggccga ccatatgacc gccatgctcg
     2041 acgaaacgcg gctgttggcg cggctggtcg atgatctgca aacgctgtcg ctggccgagg
     2101 ccgggcagct gccgctccat cgccagaccc tgcccgcggc cgacctgctg gcggatgtgg
     2161 ccacccgttt tgccgccgcc gcggccgacg cgggggtggc gctcgaccta ttgcccggcg
     2221 gggatgccat caacgttgag gtcgatcccg accggctgga tcaggcgctg tccaatctgg
     2281 tggccaacgc gctgcggcac acgccggcgg gcggccgcgc caccctgtcg gccgagcggg
     2341 cggcgggcgg cgtcgagctg gtcgtggccg acaccggcgc gggcatcgag gcggccgatt
     2401 tgccgtatat cttcgaccgc ttctggcgcg gcgaccgcgc gcgggggcga ggcggctccg
     2461 gcctcggcct gcccatcgcc cggcggctca tcgaggccca cggcgggacg atcaccgtcg
     2521 atagcgccgt gggcgagggg acgcgctttg tggtgtggct gaaggaagcg acgagtgacg
     2581 agtgacgagt gaagaccaca ggcgacagac cacgggccac atgtgacgag tgaagagggt
     2641 acgatttacg aagactgccg acaaatggtg ccggagaccc acagcttcca ggcgccggcc
     2701 ttctatcagg cgttggggtt ccgcatcgtc gggactgttg agaactatcc ggtcgggcaa
     2761 caatattaca tgccggcgaa gcaattataa atatctgtcg cgcggagccg cagggacgcg
     2821 gagaagaatg gaaaggtttt ctcagcgcct cagcgcctct gtgcgagcta tcggctaaat
     2881 tgcccgaatg aattgccgaa attggccaaa attggccgtt ttatgtcggc tggattgcag
     2941 aactccccgc tatttttcat tatactggtc acaggttacc cactttgaca ccgccaccca
     3001 ttgttcgtag tagggccttt aggcccgctc cggggaacgg cgctaaagcg cctga
//
LOCUS       DAOMQG010000386         3049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13026_length_3049_cov_10.631931, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000386 DAOMQG010000000
VERSION     DAOMQG010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13026_length_3049_cov_10.631931"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLC06_14905"
     CDS             complement(<1..354)
                     /locus_tag="PLC06_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91785.1"
                     /translation="MAAIEVKPEKGPDSRRERPTTVGGTALRLLGLIIFDAFALFFIY
                     LLLSDGYWPLASIIGAITLVINYVFLSPDAYPMRWMSPGLAFMLLISVYPILYTIYIS
                     FTNFGAANLLPKVQAI"
     gene            complement(544..2292)
                     /locus_tag="PLC06_14910"
     CDS             complement(544..2292)
                     /locus_tag="PLC06_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRO91786.1"
                     /translation="MKNTKWYLLVVVLLLGAFALAACGGGQAATPTQAPAPTEEAAPA
                     PTEAPAPTEAPAEATEAPAPTEAPAAPTLASDTSKTMLELLATDPNFSKFVEAMSAAG
                     IADPAADSPQTWFVPTNAAFDAADQSILEGFMDNLLPFHTVNGAKKAADLSGEVDTLA
                     GEPLDTSAVTITTPDVEASNGVIQVIDTVLVPPTVTNTIRAEELAAEGSPVIRIWADN
                     TRRPALLEVEAAFEEEYGVELLIEQVGFGDIRRLITTAGPAGEGPDIIVGAHDWLGEL
                     VAGGILAPIDLGDKVSEFAPAAISAFTYDGELYGMPNATENLAMFINTDLVPECPATW
                     TEVHDISADLHAADAEKYGFVRMEGDPYHFYPIQTAFGGYIFGRNETTGAYDPTNVGV
                     GDPGSIAAAKWYETMVKEGLQPPAVDWDTMHVWLETGKSAMTLTGPWALPRITESGIP
                     FKICDIPGETEEHGKPFLGAQGFMVSAFSRDPLMAQIFLTEFVATPEVMQALYDADPR
                     TPAFLPVLETLDDPNIAAFASAGANADPMPAIPEMGSVWDAWGNAVVLISQGAAPAEE
                     AFTNAQQQIITAINGG"
     gene            complement(2533..2988)
                     /locus_tag="PLC06_14915"
     CDS             complement(2533..2988)
                     /locus_tag="PLC06_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRO91787.1"
                     /translation="MTDKVHLYDLIKESFLLLDFGDRLLLEKFALTVPRYYALTHIAD
                     EPGLLPSSLSRRMFCDKSNITRLIQGLANDGLIERRPHETDGRAQRLYLTDTGARLHG
                     QAAAAHRRYVEARLKALEDCAEDQVTDILAFLNQSLADGLDIDPLTLVN"
BASE COUNT          592 a          845 c         1019 g          593 t
ORIGIN      
        1 gatggcctgc accttgggca agaggttggc cgcgccgaag ttggtgaacg agatgtaaat
       61 ggtgtagagg atcggataga cggagatcag cagcatgaat gccaggccag gcgacatcca
      121 gcgcatggga taggcatcgg gcgagaggaa tacgtagtta atgaccaggg tgatggcgcc
      181 gatgatgctt gccagcggcc aataaccgtc ggacaacagc agataaatga aaaagagggc
      241 aaaagcgtcg aaaatgatca ggcccagaag ccgcagcgct gttccgccga ccgtggtcgg
      301 ccgttcgcgc cgggaatctg gacctttctc cggtttaacc tcaatggcgg ccatgaattt
      361 cctccttcat ggtaactccc gaatgataat gtacgacaac gactcccgac gaaggccaag
      421 cccgattcgc caagaatatg ggcgattgcg ggactgatga aatcatcagc ctcgcaatcg
      481 tgctaaaacc aattgcggcg gcctcttcat tcaaagaggc cgccgcgaat caaactttgg
      541 gcattagccg ccgttgatgg cggtgatgat ctgctgctgg gcgttggtga acgcttcctc
      601 ggccggggcg gcgccctggc tgatcaggac aacagcgtta ccccaggcat cccagaccga
      661 tcccatttcg gggatggccg gcatggggtc ggcgttggcg ccggcggaag caaaggcagc
      721 gatgttcggg tcgtcgagcg tctcgagcac gggcaggaag gccggggtgc gcgggtcggc
      781 atcgtagagg gcctgcatga cttcgggcgt agccacgaac tcggtcagga agatctgcgc
      841 catgagcggg tcacgggaga aggcgctgac catgaagccc tgggcgccca ggaacggctt
      901 gccgtgctct tccgtctcgc cggggatgtc gcaaatcttg aagggaatgc cggattcggt
      961 gatacgcggc agcgcccacg ggccggtgag ggtcatggcc gacttgccgg tctcgagcca
     1021 aacgtgcatg gtgtcccagt cgacggccgg gggctggagg ccttccttga ccatcgtctc
     1081 gtaccacttg gcggcggcga tggaaccggg gtcgcccacg ccgacgttgg tcgggtcata
     1141 ggcgccggtg gtctcattgc ggccgaagat gtagccgccg aacgcggtct ggatgggata
     1201 gaagtgatac gggtcgcctt ccatacgaac gaagccgtac ttctcggcgt cggccgcatg
     1261 caggtcggcc gagatgtcgt ggacttcggt ccaggtggcc gggcactcgg gaaccaggtc
     1321 ggtgttgatg aacatggcca ggttctcggt ggcgttgggc atgccgtaca gctcgccgtc
     1381 ataagtgaag gcgctgatgg ccgccggagc gaattcgctg accttgtcgc cgaggtcgat
     1441 cggggccagg atgccgccgg cgaccagctc gccgagccag tcgtgggcgc cgacgatgat
     1501 gtccgggcct tcgccggccg ggccggcggt ggtgatcaga cgacgaatgt cgccgaagcc
     1561 gacctgctcg atgagcagct cgacgccgta ctcttcctcg aaggcggctt caacttccag
     1621 cagcgccggg cgacgggtgt tgtcggccca gatgcggatg acggggctgc cttccgcggc
     1681 cagttcttcg gcgcggatgg tgttggtgac ggtgggcgga accagcacgg tgtcaatgac
     1741 ctggataacg ccattggaag cctcgacgtc gggggtcgtg atggtcacgg ccgacgtgtc
     1801 cagcggctcg ccggccaggg tgtcaacttc accggacaga tcggcggcct tcttggcacc
     1861 gttgacggtg tggaagggca ggaggttgtc catgaagcct tccaggatgg actggtcggc
     1921 agcgtcaaag gccgcgttgg tgggcacgaa ccaggtctgc ggggaatcgg ccgcaggatc
     1981 ggcgatgccg gccgcgctca tggcctcgac gaacttgctg aagttcgggt cggtggccaa
     2041 cagctcgagc atggtcttgg acgtgtcgct ggccagagta ggcgcggcag gggcctcggt
     2101 cggggccggg gcttcggtcg cctcggccgg agcctcagtc ggagccggag cttcggtcgg
     2161 cgcgggggcc gcttcttcgg tgggcgcggg ggcctgggtc ggggtcgccg cttgcccgcc
     2221 gccgcaggcc gccaatgcaa aggcgcccag cagcagcaca acaactaaca ggtaccactt
     2281 ggtgtttttc atcattctcc tcaaattaag gtttttcatt agttaccctt tgggcaacct
     2341 aacaaacaaa acgatagtcg atgaacggaa gtcaggcttc ttcgaaacac atcacctcca
     2401 tattatcctg tgccgtttgt tgttggacag gagtatacac gtctctacag caaatctgta
     2461 atgatcgctc gccttgaccc aaccgccatt ttagggatgc tggcgggttt gaccaatcag
     2521 atgcagccaa ttttaattta ccagggtcag cggatcgata tccagcccat cagccaacga
     2581 ttgattcagg aaggccagga tgtcggtcac ctgatcctcg gcgcagtcct ccagcgcctt
     2641 gagacgggct tcgacgtaac ggcgatgggc ggcagcggcc tggccgtgca gtctggctcc
     2701 ggtgtccgtc aggtagagtc gttgggcgcg gccgtccgtc tcgtgcggcc gtcgctcgat
     2761 caggccgtca ttcgccaggc cctggatcag gcgcgtgata ttgcttttat cgcaaaacat
     2821 gcgccggctg agcgagcttg gtaataggcc gggttcgtcg gcaatgtggg tcagggcgta
     2881 atagcgggga acggtgaggg caaacttctc cagcaaaagt cgatcgccaa aatcaaggag
     2941 gagaaaagac tccttgatca gatcgtacag atgcaccttg tcggtcatgc tcccagccct
     3001 ttcatataag ttgatactgc aactgtatca tttgtaactt tatcatagg
//
LOCUS       DAOMQG010000387         3044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13045_length_3044_cov_4.248244, whole genome shotgun sequence.
ACCESSION   DAOMQG010000387 DAOMQG010000000
VERSION     DAOMQG010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13045_length_3044_cov_4.248244"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLC06_14920"
     CDS             <1..337
                     /locus_tag="PLC06_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HRO91788.1"
                     /translation="LTLYKQYTPEEYPRYDNYDAIEVSRTAEIPYDYDGMMGVPITFL
                     DKYNPDQFEIIGMAKRGAGDPALKSKVYTKEDYKNYSDLNAGPVLITSSGLKNTYPRI
                     LIKRKGAAQ"
     gene            334..1416
                     /locus_tag="PLC06_14925"
     CDS             334..1416
                     /locus_tag="PLC06_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRO91789.1"
                     /translation="MKIELHEMTVREVAAGYVDSAEEGVVGYGGKLNIRPKYQREFVY
                     DGKQRNAVIDTIRKDFPLNVMYWVKNDDGTFEVLDGQQRTISFCQYVIGDFSLDNRAF
                     HNLTETEKEQILDYKLMVYFCEGNDKEKLDWFRIINIAGEKLTDQELRNAVYTGPWLS
                     DAKLKFSKTGCAAYGLAKDYVTGSPIRQEFLETAIKWISKGNIEAYMSAHQHDPNANE
                     LWTYFRNVIEWVKDTFTVYRKEMKGIEWGGLYDEFRDKLYDTAKLEQEIQTLLIDDDV
                     TKKKGVYAYVLTRNERHLSLRAFSDNQKREAYERQKGNCKKCGKHFEFSGMAGDHITP
                     WSKGGKTVAENCQMLCADCNRRKSDV"
     gene            1534..2313
                     /locus_tag="PLC06_14930"
     CDS             1534..2313
                     /locus_tag="PLC06_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRO91790.1"
                     /translation="MEILGIDIGGSGIKGAIVNAATGEPVTERLRVPSPAGFQPDEVV
                     AVVHDLVRQFDYSGPVGVGFPGVVMRGEVITPPTALAFPGWEGVNLAERLSAAIGRPV
                     TVGNDADVACLAEMYFGAGKGQTGLVMVFTIGTGIGSAVFHDGHIVPNSELGRLYLRG
                     RKKTIEQLASDRIREQEKLSWKVWGERLNDYFQYVELLFRPDVIIIGGGVSKKHEKFF
                     PYIKLRAKLLPAAFRNEAGIVGAAMAALDVHDPQPWELSGA"
     gene            2310..>3044
                     /locus_tag="PLC06_14935"
     CDS             2310..>3044
                     /locus_tag="PLC06_14935"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRO91791.1"
                     /translation="MITPKPGPRTVYLPGTALLPLAESLVALANSDGGLIVLGINRDG
                     RPTTEIWEEEAELALREAAALCLPPVPSRFQAVELPAGRFIGIQVPRSLELHSLSDGR
                     VLVRRRDENRPLSGEEIRTLAAGRPTADFETEPVPGAKADDLDTETLREYLDKRETRT
                     GARSSSVSQLMHEVGATDREGNPTLIGLLLFGRNPQAFLPQSGVVFVRFAGTEPRGED
                     GGIGYGRRVELTGPVARIIERAWAIVF"
BASE COUNT          640 a          974 c          955 g          475 t
ORIGIN      
        1 cctcaccctc tacaagcaat acacccccga agaatacccg cgctacgaca actacgacgc
       61 catcgaagta tcgaggacgg ccgaaatacc ctacgactac gacgggatga tgggcgtccc
      121 gattacgttc ctggacaagt acaacccgga tcagtttgag attattggta tggcaaaaag
      181 aggggccggc gaccccgcgt tgaaatccaa ggtctacacc aaggaagatt ataaaaacta
      241 cagtgattta aacgcagggc cagtgttgat aacttcaagt ggcctaaaga atacttaccc
      301 acgcatttta atcaaacgca aaggagccgc ccagtgaaaa tcgaattaca cgagatgacc
      361 gtccgtgagg tcgccgcggg ctacgtcgac agcgccgagg agggggtcgt cggctacggc
      421 ggcaagctca acatccggcc caaataccag cgcgaattcg tctacgacgg caagcagcgc
      481 aacgccgtca tcgacaccat ccgcaaggat tttccgctca acgtcatgta ctgggtgaag
      541 aacgacgacg gcacatttga ggtgctcgac ggccagcagc gcacgatcag cttctgccaa
      601 tacgtcatcg gcgacttctc gctcgacaac cgcgccttcc acaacctgac cgagaccgag
      661 aaggagcaga tactcgacta caagctcatg gtctacttct gcgagggcaa cgacaaggaa
      721 aagctggact ggttccggat catcaacatc gccggcgaaa agctgaccga ccaggagctg
      781 cgcaacgccg tctacaccgg gccgtggctg tcggacgcca agctgaagtt cagcaagacc
      841 ggctgcgcgg cgtatgggct ggcaaaggat tacgtgaccg gctcgcccat ccgccaggag
      901 tttctggaga cggccatcaa gtggatatcg aagggcaata tcgaggccta tatgtccgcc
      961 caccagcacg accccaacgc caacgagttg tggacgtatt tccggaatgt gatcgagtgg
     1021 gtgaaagaca ccttcaccgt ctaccgcaag gagatgaaag gcatcgaatg gggcgggctt
     1081 tacgacgaat tcagggacaa actgtacgac accgccaaac tggagcaaga gattcaaacg
     1141 ctgctgatcg acgatgacgt gaccaaaaag aagggggtct atgcctacgt gctgacccgc
     1201 aacgaacggc atctgagcct ccgcgccttc agcgacaacc agaagcggga ggcctacgaa
     1261 cggcagaaag ggaactgcaa gaaatgcggc aagcattttg aattcagcgg catggccggc
     1321 gaccacatca ccccgtggag caagggcggc aagacggtgg ccgagaattg ccagatgttg
     1381 tgcgccgatt gcaaccgccg caagagtgat gtttagcggc gccgggtttc tgtgcaggag
     1441 tcggcgccgg gacgcaatgc aagatcggtt ggcttgcgcc cttgcgccct tgcgttgaat
     1501 ttgcggtctg ctatcacaag gagcgaaagc taaatggaaa tactcggcat cgacatcggc
     1561 ggatcgggca tcaagggggc gatcgtcaac gcggcgacgg gcgagccggt caccgagcgg
     1621 ctgcgcgtgc cgtcgcccgc cggcttccag cccgacgagg tggtggccgt cgtccatgat
     1681 ctcgtccggc aattcgacta cagcggcccc gtcggcgtgg gctttccggg cgtggtcatg
     1741 cgcggcgagg tgataacccc gcccacggcg ctggccttcc ccggctggga aggcgtcaat
     1801 ctggccgagc ggctcagcgc ggccatcggc cgcccggtca ccgtgggcaa cgacgccgac
     1861 gtggcctgcc tggccgagat gtacttcggc gcgggcaagg gccagaccgg cctggtcatg
     1921 gtcttcacca tcggcacggg catcggcagc gccgttttcc atgacggcca catcgtcccc
     1981 aactccgagc tgggccgcct ctacctgcgc ggccgcaaga agaccatcga gcaactcgcc
     2041 tccgaccgca tccgcgagca ggagaagctg agctggaagg tgtggggcga gcggctgaac
     2101 gactacttcc agtacgtcga attgctcttc cggcccgacg tgatcatcat cggcggcggg
     2161 gtcagcaaga agcacgagaa gttcttcccc tatatcaagc tgcgggccaa actgctgccg
     2221 gccgccttcc gcaacgaggc gggcatcgtc ggcgcggcga tggccgccct cgacgtccac
     2281 gacccgcagc cgtgggaact gtccggcgca tgatcacccc caagcccggc ccgcgcaccg
     2341 tctatctgcc cggcacggcg ctgctgccgt tggccgagtc gctggtggcg ctggccaaca
     2401 gcgacggcgg cctgatcgtg ctgggcatca accgcgacgg ccgccccacg accgagatat
     2461 gggaagaaga ggccgaattg gcgctgcgcg aggcggccgc cctgtgcctg ccgcccgtgc
     2521 ccagccggtt ccaggcggtg gaactgcccg ccggccgctt catcggcatc caggtgccgc
     2581 gcagcctgga actccatagc ctgagcgacg gccgcgtgct ggtgcgccgc cgcgacgaga
     2641 accggccgct gtcgggcgag gagatccgca ccctggccgc cggccgcccc acggccgact
     2701 tcgagaccga gccggtcccc ggcgccaagg ccgacgacct cgacaccgag acgctgcgcg
     2761 aatatctgga taagcgcgag acgcgcaccg gcgcgcgctc gtcgtcggtc tcgcaactga
     2821 tgcacgaggt cggcgcgacc gaccgcgagg gcaacccgac gctcatcggg ctgctgctgt
     2881 tcggccgcaa tccgcaggcg ttcctgcccc agagcggcgt cgtgttcgtc cgcttcgccg
     2941 gcacggaacc gcgcggcgaa gatggcggca tcggctatgg ccggcgcgtc gaactgaccg
     3001 ggccggtggc gcgcatcatc gagcgcgcct gggccatcgt cttc
//
LOCUS       DAOMQG010000388         3042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13061_length_3042_cov_4.736860, whole genome shotgun sequence.
ACCESSION   DAOMQG010000388 DAOMQG010000000
VERSION     DAOMQG010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13061_length_3042_cov_4.736860"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="PLC06_14940"
     CDS             complement(<1..1825)
                     /locus_tag="PLC06_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91792.1"
                     /translation="MPFRRYIRLILPAVALILMVACGGQSVQTTPAPIPPTDAPAATE
                     APATAEPAPTAEIEVAPTDEAPDGLPAGVFYDLGEATIIQANFPEDSRFRNMPVRLNG
                     VLSVPESGDGPFPVVLILHGTHPGCPVNDMGVDAWPCDPMEERDNYAGFEYLVRELAA
                     RGYVALAININAENTFGFGEPVGGERLLQLVDLHLSALAEAAAGGANNFGVELDGVAN
                     VQQLTLMGHSRGGDMVNWLAGDRLNGPNLAAPDAFATHGYGPVNGLLLIAPAVALFGS
                     EGTAVPLAAIISACDGDVIDGAGQDFYEQVRLDEGSTALAAGVVLAGANHNGFNTTLG
                     GDGFPPTDRPDCQTLLEPEAQRQFLVDYAADFLTLLDSPDAAERSAAAARLGLDATAP
                     APAELYGQAVQLASHVAAADRLTVFVPADEGDMTTNRLGGTIIADNITNQYCEMGYST
                     PQSNPGSEPCLRPNVTIPGYPAMAVVNWEGEGGAWRFELPEGARDLSGYTTVSLRAAV
                     DPISPLNVSGEPQALSIRLTDGAGNTAIVATRPDEPALVFPVGATREDEFFGQVFSSL
                     VPLTTIRLPLDGFAGVNLSDVTEIAILFDQTASGNLFLGDLE"
     gene            2032..2745
                     /locus_tag="PLC06_14945"
     CDS             2032..2745
                     /locus_tag="PLC06_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRO91793.1"
                     /translation="MNQPPPAPEPIDAVILISAHAEWRPVRERYAAIPPFATPYGEAF
                     QAALDAGGRPRQVVFFHGGWGKIDAAAGTQYAIDRWRPSLLVNLGTCGGFAGDIAAGE
                     VLLVDFTLVYDILEQMGDPDEALAHYATEIDLSWLPEPLPLPVRRELLISADRDIVAG
                     EVAGLRDRFGAIAADWESGAIAHVAARNGTRCLILRAVTDLVDYKDGEAYGRPELFVT
                     RTEAVLARLLDSLPKWITN"
BASE COUNT          492 a          998 c         1058 g          494 t
ORIGIN      
        1 gctccagatc acccaggaac agattcccgc ttgccgtttg gtcaaacagg atagcgatct
       61 ccgtcacatc gctcaggttc acccccgcga agccgtccag cggcaggcga atggtcgtca
      121 acggcacgag gctgctgaag acctggccga agaactcgtc ctcccgcgtc gcgccgacgg
      181 ggaagaccag cgccggttcg tcgggccgcg tagcgacgat ggccgtgttg cccgcgccgt
      241 cggtcagccg aatgctgagg gcctgcggct cgccggagac gttcagcggc gagatcgggt
      301 ctacggccgc gcgcaggctg accgtggtat agccgctcag atcgcgcgcc ccttccggca
      361 gttcgaagcg ccacgcgccc ccttcccctt cccaattgac gaccgccatg gccgggtagc
      421 cggggatagt gacgttcggc cgcaagcacg gctcggagcc ggggttggac tgcggggtgg
      481 agtagcccat ttcgcaatac tggttcgtga tgttgtcggc gatgatcgtc ccgcccagcc
      541 ggttggtggt catgtccccc tcatccgccg ggacgaatac ggtcaggcgg tcggcggcgg
      601 cgacgtggct cgccagttgt accgcctggc catagagttc ggccggggcc ggcgcagttg
      661 catcaaggcc cagacgagcg gcggcggccg aacgctcggc cgcgtcgggg ctgtcgagca
      721 gggtcaggaa atcggcggca taatcgacca ggaattggcg ctgcgcctcc ggctccagca
      781 gggtttggca atcgggccgg tcggtcggag gaaagccgtc gccgccgagc gtggtgttga
      841 agccgttgtg gttggccccg gccagcacga cgccggcggc cagggcagtg gagccttcat
      901 cgaggcgcac ttgctcatag aaatcctgcc cggccccgtc gatgacatcg ccgtcgcagg
      961 cggagatgat cgccgccagc ggcacggccg tgccctcgga accgaacagg gccacagccg
     1021 gggcgatgag cagcaagccg ttgaccgggc cgtaaccgtg ggtggcgaag gcgtcgggtg
     1081 cggccaggtt cggcccattg aggcggtcgc cggccagcca gttgaccata tccccgccgc
     1141 gcgagtggcc catcaaggtc agttgctgca cgttggccac gccgtccagc tcgacgccga
     1201 aattgttcgc gccgccggcg gccgcctcgg ccagcgccga caggtgcaga tcgacgagct
     1261 gcagcagtcg ctcgccgccc accggctcgc cgaagccgaa ggtgttctcg gcgttgatgt
     1321 tgatcgccag ggccacatag ccgcgggcgg ccagctcgcg cacgaggtat tcgaacccgg
     1381 cgtagttgtc gcgctcctcc atcgggtcac agggccaggc gtcgacgccc atgtcgttga
     1441 ccgggcagcc gggatgggtg ccgtggagga tgaggaccac cgggaacggg ccgtcgcccg
     1501 attcgggcac ggagaggacg ccgttgagcc gcacgggcat gttgcggaag cggctgtctt
     1561 cggggaagtt ggcctggata atggtcgcct cgcccaggtc atagaagact ccggcgggca
     1621 gcccgtcggg agcctcgtcg gtcggggcga cttcgatttc ggccgtgggc gcgggttcgg
     1681 cggtggccgg ggcctctgtc gcggcgggcg cgtcggtcgg cgggatgggt gcgggcgtag
     1741 tctggaccga ctgcccacca caagcgacca tgagaatcag ggccacggcg ggcaggatca
     1801 aacgaatata tcgacgaaag ggcatagact tctctccccc tgagcgcgac gacggcgctc
     1861 ggaataaggt aaacgatcgg ggaattataa caaggttggc gagatacata tagcgcctca
     1921 cccggctttc atgctcagcc gcgaaggcag gatacgaaag agggcgcgct tccggactcg
     1981 tatctcgtat ttcgtatctc gtatctgaag cacccgcgct ataatcccgc catgaaccag
     2041 ccaccccccg cccctgaacc gattgacgcc gtgatcctca tctccgccca cgccgaatgg
     2101 cggccggtgc gcgaacgcta tgccgccatc ccgccgttcg ccacccccta cggcgaggcg
     2161 ttccaggccg cgctcgacgc cggcggccgc ccccgccagg tcgtcttctt ccacggcggc
     2221 tggggcaaga tcgacgccgc cgccgggacg cagtacgcca tcgaccgctg gcggccgtcg
     2281 ctgctggtca atttgggcac gtgcggcggt tttgcggggg acatcgccgc cggcgaggtg
     2341 ctgctggtcg atttcaccct ggtctacgac atcctggagc agatgggcga ccccgacgag
     2401 gcgctggccc actacgcgac ggagatcgac ctgtcgtggc tgcctgagcc gctgccgctg
     2461 cccgtccggc gcgagctgct catctcggcc gaccgcgaca tcgtggccgg ggaggtcgcc
     2521 ggattacgcg accgcttcgg ggccatcgcc gccgactggg agagcggggc catcgcccac
     2581 gtcgccgccc gcaacgggac gcgctgcctc atcctgcgcg ccgtcaccga cctggtggat
     2641 tacaaggacg gcgaggccta cggccgcccc gaactgttcg taacccgcac cgaagcggtc
     2701 ctggctcgcc tgctcgattc gctgccgaaa tggatcacga attaggtcat gccggacctg
     2761 gtgatgattg gcgtcgtggg cggcaatggt cgtcgtggcc cgtcatgggc tgctcgagac
     2821 gtccatgcta caaccacccg gatcgccgct ctgcgcgtgg acgatgaggt tgccgtcgga
     2881 agaatgagac gggccggcag gatggtctcc agacctacgg atagctggcc gggatgagcg
     2941 tcagcgggtc gatgttcatc cccagccgct tgtcatagac gttgagatgg gtgtgatagc
     3001 cgcaatcgcg cccggcgcat gagttgccgg ccatgtcgag gg
//
LOCUS       DAOMQG010000389         3026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13163_length_3026_cov_7.356109, whole genome shotgun sequence.
ACCESSION   DAOMQG010000389 DAOMQG010000000
VERSION     DAOMQG010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13163_length_3026_cov_7.356109"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..>3026)
                     /locus_tag="PLC06_14950"
     CDS             complement(100..>3026)
                     /locus_tag="PLC06_14950"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO91794.1"
                     /translation="NIILKRLSRPDEAGAQPFRALLTDFGLVKLQEGNSLTQSGATVG
                     TPTYMSPEQCEGRELDNRSDLYSLGIVLYELIANRLPFTFQTLTEAIAAHRNGLTPPP
                     VSSVRPEVPPIIDALLERATAKAPGDRFATGAQMADALRSAFVALEGLPTRVMLHQEL
                     DILDQVAEPPVGFELQIQTPGHQDSVVPLTRSVITLGRNLDNDIVLPADGVSRHHTRL
                     QATSLGWEAVDMGGVNGTWLNEHRLRPEEPTPLSVGSVLRIGPYEMILLGPEAPLGAG
                     DEIKGEPISAPITPGLVGVGLVTAPNVGRSSTSATPPTASQVAEQPTIPPTQVSAPLA
                     LHLARNTFAVDPGKRLEIKVDVENRGDQDDRVSLRVAGLEPSWVEVPETFVTVPPRQT
                     ISVPFAVTPPRSRGVPVGRQRLRIEVVSQRYPQARVGIGAALTVGDFVDFEADMEPTE
                     LRLPGRVVVNIRNTGNAPGVFKVSGHDLQQLVRFRGERDGIKLQPNQTANIELALEPR
                     ENNWFGEGDTYPFDVEVSSSKGGKKSLPGEAITRALFSNSVLYGMIFLLTFACVLGGL
                     ALMTNWGRILGIGPMPTATPTLSSEQTIATQQSVAFAQTVVVATQSAAATLTPSVAGD
                     RDTDGLGDAQEAALGTNPDNPDTDEDGLKDGDEVLIYRTDPRNPDSDSDGLRDSDEIN
                     IHRTNPNLADTDADGISDHTEVALGTDPLVPNPPTSALPTSAPPTAVLPTTAPPMPSA
                     TLALPATIPPTWTAIPTAMPTAAPTATWTPSPTQAPTATFTSPPPAASLVCVPVPPVI
                     DGSLSIGVEWPGTPLFTFQPAVVGPERIVRVYGVRTGTQLYFAYLINDNVKEASDAAR
                     LYIDTTNNGLDPDSADRMFQVNRNGDTLVWPGIGSNIDGQGWDATYSSPNWSSAVGEP
                     GSGQWVVEMSINIAAELGTLADPFGLMAEVFYSGDTSAYPSAASSSQANTWQDIGNPA
                     CP"
BASE COUNT          561 a          935 c          989 g          541 t
ORIGIN      
        1 cgtaagacag ttgcataacc ccttagtcta gcacaaaaaa accgcccggc gcatttactt
       61 tcgcgccggg cggtggaaaa acgggcaagt tggcctgcat caagggcacg cagggttgcc
      121 gatatcttgc caggtgttgg cctgggatga ggaagctgcg gagggatagg cagatgtatc
      181 accggagtag aatacttcgg ccatcaggcc aaacgggtcg gcgagcgtgc ccagttcggc
      241 ggcaatgttg atcgacattt ccaccaccca ttgaccactg cccggttcgc ccacggccga
      301 cgaccaattg ggggaggagt aggtcgcatc ccaaccctgt ccgtcgatat tgctgccaat
      361 gcccggccag accaacgtat cgccgttgcg gttgacctgg aacatccgat cggccgagtc
      421 agggtccagg ccattgttgg tggtgtcgat gtagaggcgc gccgcgtccg aggcttcttt
      481 cacgttgtcg ttgatgagat aggcgaaata gagctgcgtc ccggtgcgca caccatagac
      541 gcgcacgatg cgctccgggc cgaccaccgc cggctggaag gtgaacagcg gcgtgcccgg
      601 ccattccaca ccgatgctga gcgaaccgtc gatgaccgga ggcaccggga cgcaaaccag
      661 cgaggctgcg ggcggaggcg atgtaaaggt agccgtcggc gcttgagtag gggatggcgt
      721 ccaggtggcc gtcggcgcgg ccgtcggcat ggcagtgggg atcgccgtcc acgttggcgg
      781 tatcgtcgcc gggagcgcca gtgtagccga cggcatcggc ggggcggttg tcggcagcac
      841 ggccgtcggt ggcgcggacg tcggtagcgc ggacgtcggc gggttgggga caagcggatc
      901 cgttcccaga gccacctccg tatggtcgga tatcccgtca gcgtcggtgt cggcaagatt
      961 ggggttggtt ctgtgaatgt tgatctcgtc agaatcgcgc aatccgtcgc tatcggaatc
     1021 ggggttgcgc gggtcggttc tgtagatcag cacctcgtcg ccgtctttca ggccatcctc
     1081 gtcggtgtcg ggattatcgg gattggttcc caacgccgct tcctgcgcat cacccaggcc
     1141 atcggtatcg cgatcgcccg ccacgcttgg ggtcagcgtc gccgcggcgc tctgcgtggc
     1201 caccacgacc gtctgggcaa aggccactga ctgttgggta gcgatcgtct gttccgacga
     1261 caaggtcggc gtcgccgtcg gcatcggtcc aatccccagg atacgccccc aattagtcat
     1321 cagcgccagc ccgcccagca cacaggcgaa cgtcagcaag aagatcatgc catacaggac
     1381 cgaattcgag aaaagggcgc gggtgatagc ctcgccgggc agcgatttct tgccgccttt
     1441 actggaagac acctcgacat caaacgggta ggtgtcccct tccccaaacc agttgttctc
     1501 tcgcggctcc agagccaact cgatattggc cgtctgattg ggctggagct tgatcccgtc
     1561 gcgctcgcca cggaagcgaa caagctgctg caggtcgtgg cccgacacct tgaacacgcc
     1621 cggcgcgttg ccggtgttgc gaatgttgac gaccacccga cccggcaggc gcaattccgt
     1681 cggctccatg tcggcttcga aatccacgaa atcaccgacc gtcagcgcag cgccgatgcc
     1741 gaccctggcc tgcggataac gctgggacac aacctcaatg cgtaatcgct gccggcccac
     1801 cggcacaccg cgactgcgcg gtggcgtgac ggcaaacggc acggagatcg tctggcgcgg
     1861 gggcacagtg acaaatgtct ccggcacctc aacccatgac ggctccagcc ccgccacgcg
     1921 cagactgact cgatcatcct ggtcgccgcg attctcaacg tccaccttga tctccagacg
     1981 cttgccggga tcaacggcga aggtatttcg cgccagatgg agggccaatg gcgcactgac
     2041 ctgagtcggc ggaatcgtgg gctgctccgc cacctgagag gctgtcggtg gggttgccga
     2101 tgtggatgat cggccgacat tgggcgcagt gacgaggccc acgccgacca gccccggtgt
     2161 gatgggcgcc gagatcggct cgcccttgat ctcatcgccc gcgccaagcg gcgcttccgg
     2221 gccgagcaaa atcatctcat acggcccgat gcgcaggaca gaacccacgg ataaaggcgt
     2281 cggctcttcc ggcctgagtc ggtgctcatt cagccaggtg ccgttaaccc cacccatgtc
     2341 gaccgcctcc cagccgagag acgtggcctg gagtcgagta tggtggcgcg agacgccatc
     2401 ggccggcaag acgatgtcgt tatcaagatt acggccaagg gtaatgacgg agcgcgtcag
     2461 cggcacaacg ctgtcctgat ggccgggggt ctgaatctgc aactcgaatc ctaccggcgg
     2521 ctcggccacc tgatcgagga tgtcaagctc ctgatggagc atgacccgcg tcggcaagcc
     2581 ttcgagggcg acaaatgcgc tgcgtagcgc gtcggccatc tgcgcgccgg tcgcaaagcg
     2641 gtcgccgggg gccttggctg tcgcccgctc cagcagcgca tcgatgatcg gcggcacttc
     2701 gggtcgcacg ctgctgaccg gcggcggggt caggccattg cggtgggcag caatggcctc
     2761 cgtcagggtc tggaaggtga agggcaggcg gttggcgatc agttcataga gaacgatgcc
     2821 gagtgagtac aggtcggagc gattgtccag ctcgcgacct tcacattgct ccggcgacat
     2881 ataggtcggc gtgccgacgg tagccccgct ctgggttagg ctgttgccct cctgcagctt
     2941 gaccagacca aaatcggtca acaaggcacg aaacggttgc gcgccggctt catccgggcg
     3001 gctgaggcgc ttcaggatga tattgc
//
LOCUS       DAOMQG010000390         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13198_length_3022_cov_9.207280, whole genome shotgun sequence.
ACCESSION   DAOMQG010000390 DAOMQG010000000
VERSION     DAOMQG010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_3022_cov_9.207280"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..553
                     /locus_tag="PLC06_14955"
     CDS             197..553
                     /locus_tag="PLC06_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HRO91795.1"
                     /translation="MQTVALIAVLIVAAIHVYILWLEMFAWDTPRGRKVFGTTPEFSA
                     ASKTLAANQGLYNGFLAAGLVWSAFMGVAGRPIAIFFLCCVIVAGLYGAATASRKILY
                     VQALPAAIALGLVLLS"
     gene            complement(638..1384)
                     /locus_tag="PLC06_14960"
     CDS             complement(638..1384)
                     /locus_tag="PLC06_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HRO91796.1"
                     /translation="MDAPTTFTIPTSFEYFAIFLWATSGAIVGWRKGYDIIGVFVVAL
                     VSSFGGGLLRDGLFLQRLPVVLTDSTYYLIMGAAVIGVVAVGRRINRDRLLPKIVSVI
                     DALGVPMFVVIGVELARGRALEIPAVVLLGTVSGVGGGLLRDLIVGDTPELLRPGQYN
                     TLLVVLAALAYIVIAYEMGYNRFYTAWFVVIAFFVARLITIHYNWRSRPVNEFEMRHV
                     LEGMTGWIPGWKKAGEEEEEKKEEGGSVDQ"
     gene            complement(1448..1954)
                     /locus_tag="PLC06_14965"
     CDS             complement(1448..1954)
                     /locus_tag="PLC06_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRO91797.1"
                     /translation="MKIAIGSDERTHVTDAVMAELRRRGHELLPVGPLLDLTGGATEA
                     SISEGIPAAPPVRSDHLWPAVGRAVAEAVASGAADEGILFCWTGTGVSIAANKVPGIR
                     AALCDDAETARGARLWNDANVLCLSLRRTSEAVAKEILNTWFDTSFISNDTDNACLAQ
                     LNDMENKG"
     gene            complement(2081..2689)
                     /locus_tag="PLC06_14970"
     CDS             complement(2081..2689)
                     /locus_tag="PLC06_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRO91798.1"
                     /translation="MYDYRKLTDEQKQELTASRKARGFPIHSPPHFQVDQAIYLLTAA
                     CYEHRNHMTAPERRAELLDRLFDETINAGLSIHAWVILPNHYHILAYAEDFHKLSNVF
                     GRAHGSTAFAWNGADGKRGRRVWFRFSDRMIRSEAHYLTTLNYIHYNPVKHGWAGSPY
                     DWVESSVHWYLANYDREWLRDLWARYPLKAYGTSWDGDFDID"
     gene            complement(2793..>3022)
                     /locus_tag="PLC06_14975"
     CDS             complement(2793..>3022)
                     /locus_tag="PLC06_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91799.1"
                     /translation="VLSHSKKKIRAKALTTNKEAHCSSLVVKRFSAVLSHSKKKNRAK
                     ALTTNKEAHCSSLVVKRFSAVPPHFQEEESR"
BASE COUNT          597 a          874 c          912 g          639 t
ORIGIN      
        1 gaaatccgtg tcgtccgcgt tctgttcgct ccttcatctt ttcatgcgat tgccccgaca
       61 ggcggcgcgc tgttgcgccc acctgtcggg tctttcaccg tggccgtcgt cgcccggctt
      121 gaccccgcgc ctctctcccg ctaccattcc ccatcctgtt caccgttcac gaagcccgca
      181 ttcttgaggt ctcgccatgc aaaccgtcgc cctgattgcc gttctcatcg tcgctgcgat
      241 ccatgtgtat atcctgtggc tggagatgtt cgcctgggac acgccgcgcg gccgcaaggt
      301 gttcggcacg acgccggagt tttcggccgc gtcgaagacg ctggcggcca atcaggggct
      361 gtataacggg tttttggctg ccgggctggt ctggagcgcg ttcatgggcg tggccgggcg
      421 gccgattgcc atcttcttcc tgtgttgcgt catcgtcgcc ggtctctatg gcgcggcgac
      481 ggcgagccgg aagattctgt atgtgcaggc gctgccggcg gcgattgcgc tggggttggt
      541 attgttgagt tgagttttgg ggggcgccag acctgacagg tgggcgcacg aaggctctcg
      601 ttaaaccaac aacctcatgc gcccacctgt caggtctcta ctgatccact gacccaccct
      661 cctccttctt ctcctcttcc tcctcccccg ccttcttcca ccccggaatc cagccggtca
      721 tgccttccag cacgtggcgc atctcgaact cgttcaccgg gcggctgcgc cagttgtagt
      781 ggatagtgat gagacgggcg acgaagaagg cgatcacgac gaaccaggcg gtgtagaagc
      841 ggttgtagcc catttcgtag gcgatcacga tgtaggccag agcggcgagg acgacgagca
      901 aggtgttgta ttggcccggc cgcagcagct ccggcgtgtc gcccacgatc aggtcgcgca
      961 gcaaaccgcc gccgacgccg gagacggtgc cgagcaggac gacagccggt atctccaggg
     1021 cgcggccgcg ggccagttcg acgccgatga cgacgaacat gggcacgccc agcgcgtcga
     1081 tgacggagac gattttgggc aacaggcggt cgcggttgat gcggcggccg acggccacca
     1141 ccccgatgac ggcggcaccc ataatcaggt agtaggtgga gtcggtcagc accacgggca
     1201 ggcgctgcag gaacagcccg tcgcgcagca ggccaccgcc gaacgaggac accagggcga
     1261 cgacgaacac gccgatgatg tcgtacccct tgcgccagcc gacgatggcc cccgaggtgg
     1321 cccagagaaa gatggcgaag tattcgaatg aggtggggat ggtgaaggtg gtgggtgcgt
     1381 ccatgattac gagatacgag atacgagata cgagtgaaga acgcgcttcc gaactcgtat
     1441 ttcgtattta tcctttgttt tccatgtcgt ttagttgcgc caggcaggcg ttatcggtgt
     1501 cgttggagat gaacgaagta tcgaaccacg tgttcagtat ttctttggcg acggcttcgc
     1561 tcgtccggcg cagagagagg cagaggacgt tggcgtcatt ccacaggcgc gcgccgcggg
     1621 cggtctcggc gtcgtcgcac agggcggcgc ggatgccggg aaccttgttg gcggcgatgc
     1681 tgacgccggt gccggtccag caaaagagga tgccttcgtc ggccgcgccg gaggcgacgg
     1741 cctcggccac cgcgcggccg acggcgggcc agaggtgatc agacctgaca ggtggcgcgg
     1801 ccgggatacc ttcgctgatt gaagcctccg tcgcgccacc tgtcaggtcc agcaacggcc
     1861 cgacaggcag cagctcgtgg ccgcgccgcc gcagctcggc catgaccgcg tcggtcacgt
     1921 gggtgcgttc gtcggagccg atggcgattt tcatgaaaga tagtgtagca gaaattggga
     1981 attaggggat gaccgcagac ggccgatcgc gtgaggaacg ggcggcctcc gcccaatcct
     2041 tgcctcctgt tgatagtcaa gcgcggcaac tacaaaccaa tcaatcaata tcaaaatccc
     2101 catcccaact tgtgccatag gccttcaatg ggtatcgcgc ccataaatcg cgcaaccatt
     2161 cgcggtcgta attcgccaag taccaatgaa cgctactttc cacccaatca tagggagaac
     2221 cggcccaacc atgtttcacc ggattataat gaatgtaatt cagggtcgtc aggtaatgag
     2281 cctccgatcg aatcatgcgg tcgctgaaac gaaaccagac ccttcgccct ctcttcccgt
     2341 cggccccatt ccatgcgaaa gccgtcgagc catgcgctcg gccaaatacg ttgcttagct
     2401 tgtgaaaatc ctctgcatag gccaggatat ggtaatgatt cggcaaaata acccacgcat
     2461 ggatcgacag acccgcgttg attgtttcat cgaagaggcg gtccagtaac tcagcccgcc
     2521 gctccggagc cgtcatgtgg ttcctgtgct catagcaagc ggccgtcagt aaataaatgg
     2581 cctgatcgac ctgaaagtgc ggcggcgaat ggataggaaa tccgcgagcc tttctcgatg
     2641 cggtcaattc ctgcttttgt tcatcggtga gcttgcgata atcatacatg gcgattttgt
     2701 ggcctatata aatgggatgc ccattgtaga acgattatca gttgtcccca acttttcgtc
     2761 agtaatcaag ctcttcgctc gtagtaagcg ctttagcgcg attcttcttc ttggaaatga
     2821 gggggaacag cgctgaagcg cttaactacg agcgaagagc agtgtgcctc tttgtttgta
     2881 gtaagcgctt tagcgcgatt cttctttttg gaatgagaaa gaacagcgct gaagcgctta
     2941 actacgagcg aagagcagtg tgcctccttg tttgtagtaa gcgctttagc gcgaatcttc
     3001 tttttggaat gagaaagaac ag
//
LOCUS       DAOMQG010000391         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13256_length_3012_cov_10.744674, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000391 DAOMQG010000000
VERSION     DAOMQG010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13256_length_3012_cov_10.744674"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..551)
                     /locus_tag="PLC06_14980"
     CDS             complement(78..551)
                     /locus_tag="PLC06_14980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRO91800.1"
                     /translation="MSKPTPIINPEYVSRLMEMALDASYPRLIGMTLDDIAIDRAVIG
                     LELDPQRHLQPFGIVHGGVLATLIDTATFWAAFLRLPPDVGLVNIDLKLNYLQPAIAG
                     RLTAVGRCIRPGRTLIYTEAHIYDDSGQLLAHGTSTLISLPGRGFDLGVAKFLDS"
     gene            complement(586..1620)
                     /locus_tag="PLC06_14985"
     CDS             complement(586..1620)
                     /locus_tag="PLC06_14985"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRO91801.1"
                     /translation="MNNSSIFITGGAGFIGCNSADHFLRQGKQVTIYDNLTRKGGRSN
                     LDWLYGEHGGDRLHLIEADIRDYDRLSAAVAEAAPEVVLHLAGQVAVTSSVVDPREDF
                     EINALGTFNVLEAVRHGAPEAAVLYASTNKVYGGMEDVGIVQRGQRYEYADYPLGVPE
                     SFPLDFHSPYGCSKGTGDQYTRDYARIYGLRTLVLRQSCIYGQRQFGVEDQGWVAHFV
                     IATVMGRPIAIYGDGLQVRDVLHVDDLVAAYQAGIDRIDALSGEVFNLGGGPENTLAV
                     WTEFGPLLEELAGRPVPVHYGPWRPGDQRVFVANIQRAEELLGWRPQISPREGITRLY
                     RWVAGNPGLF"
     gene            complement(1717..2247)
                     /gene="rimI"
                     /locus_tag="PLC06_14990"
     CDS             complement(1717..2247)
                     /gene="rimI"
                     /locus_tag="PLC06_14990"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRO91802.1"
                     /translation="MLAVPTPYSLRPMAVADIPAVLEIDHLSFPTPATEQLFLNELTD
                     NQLAHYQVLARDGRELAGYAGFWLIAGEIHISTIAVRPDERGRGRGEWLLLNLLLEAW
                     ALDPILVTLEVRRSNETAQALYRKYRFEEVGLRRRYYRDTGEDAILMTIDFAQQPDYG
                     HWLAARAGAIAHATAD"
     gene            complement(2254..2913)
                     /gene="tsaB"
                     /locus_tag="PLC06_14995"
     CDS             complement(2254..2913)
                     /gene="tsaB"
                     /locus_tag="PLC06_14995"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRO91803.1"
                     /translation="MILAIDTATDWVGLALHDGSSVLAEFGWHSRRTQTIELAPAVAQ
                     LWARTGVSAGDLSAIAVAIGPGSYTGLRVGLALAKGIALGQKLPLVGVPTLDIVAAAV
                     NRLDTDLVVLAQAGRSRLWAGQYSWSDKRRWEPEGDPVLTQWDDLLGRVQVPVAFAGE
                     IDAAAAKLIRRVNRSAHIVAPAGSVRRAAVLAEIGFARWKKQQVTDANALAPTYLREP
                     G"
BASE COUNT          597 a          977 c          940 g          498 t
ORIGIN      
        1 cggccgtgcc gcagcgatgc atgacgatgc gcgatttcat cagcgccaac acgccaacct
       61 cttcagacga atgaagatca ggaatccaga aacttggcca ctcccagatc gaagccccga
      121 cccggcaagg atatcagcgt cgatgtgccg tgggccagca gttggccgct atcgtcgtag
      181 atgtgggctt cggtatagat gagcgtgcgg ccggggcgaa tgcagcgccc gacggccgtc
      241 aatcgcccgg cgatggccgg ctgcagatag ttgagcttca ggtcgatatt gaccaacccc
      301 acgtcaggcg gcaggcgcag aaaagcggcc cagaacgtgg ccgtgtcgat caacgtggcc
      361 agtacgccgc cgtggacgat gccgaaaggc tgaagatgcc gctgcggatc aagctccagg
      421 ccgatgaccg cgcggtcgat ggcgatgtca tccagcgtca tcccgatcag tctgggatag
      481 gaagcatcga gggccatctc catcagccgg ctcacatatt cggggttgat tatcggcgtg
      541 ggcttactca tacgcgaacc atccgcatcg atcatcgcga taaagctaga acaatcccgg
      601 attcccggca acccagcgat acaatcgcgt gatcccttcg cggggggaga tttgcggccg
      661 ccagccgagt agctcctcgg cgcgctgaat attggccacg aacacgcgct gatcacccgg
      721 ccgccacggg ccataatgca ccggcaccgg ccgcccggcc agctcttcca gcagtgggcc
      781 gaactcggtc cagacggcca gcgtattctc cgggccgccg cccagattga acacctcgcc
      841 gctcagtgcg tcgattcgat cgatgcccgc ctggtaggcc gccaccagat catcgacatg
      901 gagcacgtcg cgcacctgca gaccgtcgcc gtagatggcg atcggccgcc ccatcaccgt
      961 ggcgatgacg aaatgggcca cccagccctg gtcctccacg ccaaactgcc gttggccgta
     1021 gatgcacgac tggcgcagta ccagcgtgcg cagcccatag atgcgggcgt aatcacgggt
     1081 atactgatcg cccgtgcctt tcgagcagcc gtagggggag tggaaatcga gcgggaagct
     1141 ctccgggaca cccagtggat agtcggcgta ctcatatcgt tgcccgcgct ggacgatgcc
     1201 gacatcctcc atgccgccat agaccttgtt ggtggacgca tagaggacgg ccgcctccgg
     1261 cgcgccatgg cgcacggcct ccaggacgtt gaacgtgccc agggcgttga tctcgaaatc
     1321 ctcgcgcggg tcgacgaccg acgatgtgac tgccacctgc ccggccagat ggagcaccac
     1381 ctccggcgcg gcctcggcca cggccgcaga cagccggtcg tagtcgcgga tatcggcttc
     1441 gatgagatgc agccggtcgc cgccatgctc gccgtagagc cagtcgaggt tggagcggcc
     1501 gcccttgcgc gtcagattgt cgtagatagt gacttgcttg ccctgccgca agaagtggtc
     1561 ggcgctgtta cagccgataa acccggctcc accggtgatg aaaatagatg agttattcat
     1621 agcattgaaa aatcaaacag gtctgaaaaa gaagcgaggc aaacggcgac gagcgcccgg
     1681 cttgaattcc gattctattc tacacgcttt tttggcttaa tccgctgtcg cgtgagcgat
     1741 ggctcccgcc cgcgctgcca gccaatggcc gtaatcgggc tgttgggcga aatcgatggt
     1801 catcagtata gcgtcctcgc ctgtgtcgcg atagtagcgt cgccgcaggc cgacctcctc
     1861 gaaccgatac ttgcgataga gcgcctgcgc cgtctcatta ctgcgccgca cctccagagt
     1921 gacgagaatc gggtcgagcg cccaggcctc cagcaagaga ttcagcaaca gccattcgcc
     1981 ccggccgcgg ccgcgctcgt cgggacggac ggcgatggtg ctgatgtgga tctctccggc
     2041 gatgagccag aacccggcat agccggccaa ctcccggccg tcgcgggcca atacctgata
     2101 gtgggccaac tggttgtcgg tgagttcgtt caggaacaat tgctcggtcg ccggggtagg
     2161 aaaagagaga tggtcaatct ccagcacagc cgggatgtcg gccacggcca tcggccgcaa
     2221 cgaatagggc gtggggacgg cgagcatggc gatctagccc ggttcgcgca ggtaggtggg
     2281 ggccagagcg ttggcgtcgg ttacctgctg cttcttccag cgcgcgaagc cgatctcggc
     2341 cagcacggcc gcgcgccgca cggatccggc cggggcgacg atgtgggccg agcgattgac
     2401 gcggcgaatg agtttggccg ccgcggcatc gatctccccg gcaaaggcca ccggcacctg
     2461 aacccgcccc agcagatcat cccactgcgt cagcacggga tcgccttccg gttcccaacg
     2521 acgcttgtcc gaccagctat attgcccggc ccacaggcgc gagcggccgg cctgggccag
     2581 aaccacgagg tcggtatcaa gtcgattgac ggccgcggcg acgatatcga gcgtcggcac
     2641 gccaacaagc ggcaacttct gccccagcgc gatccccttg gccagcgcca gcccgacgcg
     2701 caagccggtg taggagccgg ggccgatggc aacggcaatc gcgctgagat cgccggccga
     2761 gacgcccgtc cgcgcccaaa gttgagccac ggccggagcc aactcgatcg tctgggtgcg
     2821 gcggctgtgc cagccgaact cagccaggac gctgctgccg tcgtggagtg ccagccccac
     2881 ccaatccgtc gccgtgtcaa tcgctaaaat catggtcact cagaaagaga ttctatactc
     2941 caaaagcatt tttgcgaaaa tccaccagca gaacgctcga ccgcggcccc atggcgctga
     3001 ggcgcaggcg gc
//
LOCUS       DAOMQG010000392         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13377_length_2990_cov_8.227598, whole genome shotgun sequence.
ACCESSION   DAOMQG010000392 DAOMQG010000000
VERSION     DAOMQG010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13377_length_2990_cov_8.227598"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..2506)
                     /locus_tag="PLC06_15000"
     CDS             complement(203..2506)
                     /locus_tag="PLC06_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRO91804.1"
                     /translation="MNHLRTARRSTSWWVRPILLTLILFAAIVTSVSPHFNRPVAALS
                     PDIVISQVYGGGGAAVGSTYTNDFVELFNRGATAVDVTGWSIQYASAAGTGLFGATTV
                     VITPLSGTLQPGQYLLVEGAVSTATPTGVPLPTPDVTDTSPINLSGSSGKVALVTDST
                     PLGCNGGSTPCDAGMLARIQDLVGYGATATTPPNFFEGTVPAPEPAPSASNGVQRKDG
                     GCTETDDNGADFLEAIPAPRNMASTFNPCYPDTSIDSNPTDPSNSANASFTFSSADAG
                     SMFECQLDGGGWATCTSPQNYAGLADSSHTFEVKATNMRGNTDPTPASWTWLIDTMPP
                     DTSIDSNPPDPSNSANASFTFSANEAGSSFECQLDGGGWSDCTSPQNYAGLADGSHTF
                     EVRATDAAANTDPTPASWTWLIDTMPPDTSIDSNPPDPSNSANASFTFSANEAGSSFE
                     CQLDGGGWSGCTSPQNYAGLADGSHTFEVRATDAAANTDPTPASYTWLVDIVAPPTPI
                     LVSTLSAGSVGSLNFGLEDILMWDGSNWSTWFDGSAAGLMPNGKAIHNINAFAIPDPN
                     DDDVIMAFAQNKRHVPGIALPVEGMDLVRWNGTAFSFYFDGSDVGLTSKTQEKIDALH
                     ILPGSASPINGGSCLAYLLISTPGPGKVNNPGGGALRFSGEDVLGFCMTSSGDNTAGL
                     WHLVLDGSAEGMPPNSLVNLSASDDGQVLYLTTRGAFNVDAASGGHSMVYRFDRGTGL
                     FSGPFFSAPAAGLAPGLDGLQVMGDLP"
     gene            2757..>2990
                     /locus_tag="PLC06_15005"
     CDS             2757..>2990
                     /locus_tag="PLC06_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO91805.1"
                     /translation="MYEDVIPTLKQAYDNHAGERNTADLADWKIVECEHFLALLQSEG
                     KQSLLEIGAGPGHFGAFFRDRGLDVTCTDLSPEM"
BASE COUNT          633 a          894 c          886 g          577 t
ORIGIN      
        1 ttctaacccc tgttcctcca tagtttacca gattttgtat taagcaaaat gatcatccat
       61 cacaattttt ccttaaatgg ccgcccatgg aaaggtcatg gggcagatga gttcgagacg
      121 tgcaaggcaa ccggcaagga tgacgccaca ggacgccatc cttgccgata ttaagagaga
      181 tcacgaagtc gatgcatcga tattacggta gatcgcccat cacctgcagc ccgtcgagac
      241 ccggcgccag gccggctgcc ggagccgaga agaacgggcc gctgaacaag cccgtgccac
      301 ggtcgaagcg atagaccatc gaatggccgc ccgacgccgc gtccacattg aaagcgccac
      361 gtgtcgtcag gtagagaacc tgaccgtcat cgctcgccga cagattgacc agtgaattgg
      421 gcggcatccc ctcggccgac ccatcgagaa ccagatgcca taatcccgcc gtgttatcgc
      481 cggaactcgt catgcaaaag cccaacacat cttcgccact gaatctcagc gcaccgccgc
      541 cggggttatt caccttgccg gggccaggag tgctaatgag caggtaggca aggcaactcc
      601 cgccgttgat gggcgatgcc gatccgggca ggatgtgcaa cgcatcgatc ttttcctgcg
      661 ttttgcttgt cagcccaacg tcggaaccgt caaagtagaa ggaaaacgcc gtgccgttcc
      721 agcgcaccag atccatgccc tccacaggca gcgcgattcc gggcacatgc ctcttgttct
      781 gcgcaaaggc catgatcacg tcatcatcgt tcgggtcggg aatcgcgaag gcgttgatgt
      841 tgtgtatagc cttgccgttg ggcatcaggc cggcggccga accatcgaac cacgttgacc
      901 aattgctgcc gtcccacatc aggatgtcct cgaggccaaa attcaggcta cccaccgaac
      961 ccgcgctcaa cgtggacacg aggatcggtg tcggcggcgc cacaatgtcg accagccagg
     1021 tgtaggatgc cggggtcggg tcagtgttgg cggccgcgtc cgttgcccgc acctcaaagg
     1081 tatgactgcc gtcggccagg ccggcgtagt tctgcggcga ggtgcagcca gaccaaccac
     1141 caccgtccag ttggcactca aacgagctgc ccgcctcgtt cgcgctgaag gtgaaagagg
     1201 cattggcgct gttcgacggg tcaggcggat tgctgtcgat gctcgtatca ggtggcatcg
     1261 tgtcaatcag ccacgtccag gatgccgggg tcgggtcggt gttggcggcc gcgtccgttg
     1321 cccgcacctc aaaggtatgg ctaccgtcgg ccaggccggc gtagttctgc ggcgaggtgc
     1381 agtcagacca accaccaccg tccagttggc actcaaacga gctgcctgcc tcgttcgcgc
     1441 tgaaggtgaa agaggcattg gcgctgttcg acggatcagg cggattgctg tcgatgctcg
     1501 tatcaggtgg catcgtgtca atcagccagg tccaggatgc cggggttggg tcggtattgc
     1561 ctcgcatgtt cgtcgccttc acctcgaagg tatggctgct gtcggccagc ccggcgtaat
     1621 tctgtggcga agtgcaggtc gcccaaccgc cgccgtccag ttgacactcg aacatgctac
     1681 ctgcatcggc cgagctaaag gtaaatgaag cgttggcgct gttcgatggg tcggttgggt
     1741 tgctgtcgat gcttgtgtcg gggtagcatg ggttgaacgt agaggccata ttacgcggtg
     1801 cgggaatggc ctccagaaaa tcggccccgt tgtcatccgt ttccgtgcag ccgccatcct
     1861 tccgctggac gccgtttgag gcgctgggag caggttccgg agccggaacc gtgccttcga
     1921 agaagttagg cggcgtggtg gctgtagctc catagccgac caggtcttga atgcgcgcta
     1981 gcatcccggc atcgcacggc gtcgagccgc cattgcagcc gagcggggtc gaatccgtca
     2041 ccaacgccac cttgcccgaa ctgccgctga ggttgatggg cgaagtatcg gttacgtcag
     2101 gagtgggcaa aggcacgccg gttggcgtag ccgtcgaaac cgccccctca accagcagat
     2161 actggcccgg ttgcagcgtg cccgatagtg gagtaatcac gaccgtagta gcgccaaaca
     2221 atcccgtgcc ggcggcactg gcatactgga tagaccagcc ggtcacgtca acggccgtag
     2281 ctccccgatt gaacagttcc acaaagtcat tggtgtatgt agaaccaacg gctgcaccac
     2341 cgccgccata cacctgactg atcacaatgt cgggcgagag cgccgcgaca ggtcgattga
     2401 aatgcggcga aacactggtg acgatcgctg caaacaggat aagggttaac aatatgggcc
     2461 gaacccacca cgaggtggac cggcgtgccg ttctcaagtg attcatggct acgctcctat
     2521 tcaattgatt attgaatggg ccggcgtaat cctggcgacg gctcaaaagg tgggaaggac
     2581 cctcatcctt aactcccgcc tattgcgaca actgtagaat agcatagcca aggccgcgtt
     2641 gtatatccgc caaaatacct atcctaatca cggcgataac atacaccaaa ccaggtccga
     2701 ctacagcgcc ggcgcaggtg ttcgcggcgc gtcggccaca tggtatactt tccgctatgt
     2761 acgaagacgt tatccccaca ctgaagcaag cttacgacaa ccacgccggg gaacgtaata
     2821 cagcagactt ggccgattgg aagatcgtcg agtgcgaaca ctttctggcg ctcctgcaat
     2881 cggaaggcaa gcaatcgctg ctggagatcg gcgcggggcc gggccacttc ggcgctttct
     2941 tccgcgaccg gggcctcgac gtcacctgca ccgacctctc gccggagatg
//
LOCUS       DAOMQG010000393         2958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13554_length_2958_cov_10.734757, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000393 DAOMQG010000000
VERSION     DAOMQG010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13554_length_2958_cov_10.734757"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..368
                     /gene="trxA"
                     /locus_tag="PLC06_15010"
     CDS             30..368
                     /gene="trxA"
                     /locus_tag="PLC06_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRO91806.1"
                     /translation="MAKPLDVTDATFQKEVLESDVPVLVDFWADWCGPCRFIAPFVKE
                     IAAEQDGVLKVAKVDVDDNPAVPGRYAIVGIPTLMLFKGGQVVERIVGAQPKERILAQ
                     VLPHLEKAAA"
     gene            complement(533..997)
                     /locus_tag="PLC06_15015"
     CDS             complement(533..997)
                     /locus_tag="PLC06_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91807.1"
                     /translation="MSDEKSTINELKEEERIKIDIPVDEEAAGKSTESIDIAEEFKRL
                     GRQFGATLESIFNSDEARRVEGEVRAGMKSFADEVEKVINEAKDSPTATRMKEEAAGA
                     RERVETGEFGRKAQTGMVSGLRWLSTELEKLAEQFAPADKGEEAPAEKSPTE"
     gene            complement(1157..1945)
                     /locus_tag="PLC06_15020"
     CDS             complement(1157..1945)
                     /locus_tag="PLC06_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRO91808.1"
                     /translation="MSKQQVQEQFGANAAAYVGSPVHAQGASLARLVALAAPEPGWRV
                     LDVATAAGHTALAFAPHVAAVVGLDLTPQMIPLAAALAVERGATNVNLMVGDVDDLPF
                     GDATFDAVTCRIAPHHFADIGRFIAEAVRVLRPGGLLAVVDNIVPGSRLRGKKADRQR
                     AAGDYVNIFEKLRDPSHGRCLSHDEWLDALVAAGLTVEAQETLDKRMTFETWAARHTP
                     EMQTRLRVLLLRAPAAAAEFLDVRTDTGLTTFRLREGVYLARKQ"
     gene            complement(2062..2808)
                     /locus_tag="PLC06_15025"
     CDS             complement(2062..2808)
                     /locus_tag="PLC06_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91809.1"
                     /translation="MQQENFFERFPRVPAVDLPKSASRKAVIGIILVVALVAFEIFNF
                     DTTRFALNSILGEVSFFGITWATILAIAFCAIDFAGLARLFTPERGADEPKEIWFLMG
                     AWLLGATMNAVMTWWAVSLTLLSHEFGNEVLARETLLKLVPIFVAGLVLLTRILFIGA
                     FTVAGEQIFDLTGDSRDQAAARPQPARQQPVGLPLTQPRRAPQMTLVEEEDPLPLTTH
                     RGPTGRTHQRPSTPSGTHRMPAPMAAKPRR"
BASE COUNT          551 a          906 c          902 g          599 t
ORIGIN      
        1 aatagagaac tcgaaggaga tatttgaaaa tggcaaagcc actcgatgtt accgacgcca
       61 ccttccagaa ggaagtgctg gagtctgatg tccctgtcct ggtcgacttt tgggccgact
      121 ggtgcggtcc gtgtcgtttt atcgccccgt tcgtgaagga aatcgcggcg gagcaggatg
      181 gtgtccttaa ggtcgccaag gtggacgtgg atgacaaccc ggccgtcccc ggccgctatg
      241 ccatcgtggg catcccgacc ctcatgctct tcaagggtgg ccaggtcgtc gagcgcatcg
      301 tcggcgcgca gccgaaggag cgcatactgg cccaggtgtt gccgcatctc gaaaaggccg
      361 cggcctaatc gagacaattt taagcagaaa gagcgccgcg aatagcggcg ctctttatta
      421 taagccgttg gcatcgtctg ttgagtttcg aacgtcggat gacagacgac ggaccacagc
      481 gcggaacatc gccaacgagt tgtgacccgt gtgggggttc gcgttcgatg tcttattcgg
      541 tcggcgattt ttcggccggg gcttcctcgc ccttatcggc gggggcgaac tgctcggcca
      601 gtttctccag ctcggtgctt agccagcgca aaccgctgac catgcccgtc tgggccttgc
      661 ggccgaattc acccgtctcg acgcgctctc tcgctcccgc ggcctcttcc ttcatccggg
      721 ttgcagtcgg gctatccttg gcctcattga tgaccttctc cacctcatcg gcgaagctct
      781 tcatccccgc gcggacttct ccctcaacac ggcgtgcctc atcgctattg aagatgctct
      841 ccagcgtggc gccgaactga cggccgagac gcttgaactc ctcggcgata tcgattgatt
      901 ccgtcgactt ccccgccgct tcctcgtcga ccgggatatc gatcttgatt cgttcctctt
      961 ccttcaactc attgatggta gacttttcgt cactcatggc ttgatgctcc tatgtctgcc
     1021 gcaggtcccg gatcgccgcc atgtgcaaca gcgccggaaa accagggcgg taattctctg
     1081 ttattcaata cgcgggaatg gccgtttgag ttgcgccaat taagggcagt tttggcgaac
     1141 gcgaggaaaa ccgggcctac tgcttccgcg ccagatacac cccttcccga agtcgaaacg
     1201 tggtcaggcc ggtatcggtg cgcacatcca ggaactcggc cgcggcggcg ggcgcgcgca
     1261 ggagcaggac ccgaaggcgc gtctgcattt cgggcgtgtg gcgggcggcc caggtctcga
     1321 aggtcattcg cttgtcgagc gtctcctgtg cctcaaccgt cagcccggcc gcaaccagcg
     1381 cgtcgagcca ttcgtcatgg ctgaggcagc gcccatggct tggatcgcgc agcttttcaa
     1441 agatgttgac gtagtccccc gccgcgcgtt gccggtcggc cttcttgccg cgcagccggc
     1501 tgccgggcac gatgttgtcg accacggcca gcaggccgcc cggccgcaac acccgtactg
     1561 cctcggcgat gaagcggccg atatcggcaa agtggtgggg cgcaatacga caggtcaccg
     1621 cgtcgaacgt ggcgtcgcca aagggcaaat catcgacatc acccaccatg aggtttacgt
     1681 tggtcgcgcc gcgctcgaca gccagcgccg ccgccaaggg tatcatctgc ggagtcagat
     1741 cgagaccgac gactgcggcc acatggggcg caaaggccag cgccgtatgg cccgcggccg
     1801 tggcgacgtc cagtacacgc caccccggct ccggcgctgc cagcgcgacg agccgcgcca
     1861 gactggctcc ctgggcgtga accggacttc cgacataggc ggccgcgttg gcaccgaatt
     1921 gttcctgcac ctgctgcttg ctcatgacgt tcctctttcg ctccagtata accgcataaa
     1981 aaagacctga cagatttttg gaaacctgtc aggtctgtat aaagggcggg gccacacccg
     2041 cccaacggat gaagtccacc ctcatcgccg gggcttggcg gccatcggcg cgggcatgcg
     2101 atgtgttccg ctgggcgtcg acggccgttg gtgggttcgg ccggtcgggc cgcgatgcgt
     2161 ggtcaatggt aatggatctt cttcctcaac gagcgtcatc tgcggcgcgc gccggggttg
     2221 tgtcagaggc agaccgacgg gctgttgacg ggccggctgc ggccgggcgg cggcctgatc
     2281 gcggctgtcg ccggtcagat caaatatctg ttcgccggcg acggtgaacg caccgatgaa
     2341 caggatacgg gtcaatagca ccaggccggc cacgaaaata ggcaccagtt tcagcaacgt
     2401 ttcgcgggcc aatacctcat tgccgaactc gtggctcaac agggtcagcg ataccgccca
     2461 ccaggtcatg acggcattca tcgtcgcgcc cagcaaccag gcgcccatta gaaaccagat
     2521 ctctttcggc tcgtcggccc cgcgttcggg ggtgaagagc cgggccagtc cggcgaagtc
     2581 gatggcacag aaggcaatgg ccaggattgt ggcccaggtg atgccgaaga aggatacctc
     2641 gcccaggata ctgtttagcg cgaagcgcgt cgtatcgaag ttgaagatct cgaaagcaac
     2701 cagggctaca acaaggatga tgccgatcac tgccttgcgt gaggcacttt taggcaggtc
     2761 tactgcagga actctgggga accgttcaaa aaaattctct tgctgcatgg ttcttactcc
     2821 tctttctgct cgcaggtacg ataagcctct tctcctggcc aattccttcc ggaaaaggag
     2881 aaggggccga caagttctct cttgccggcc cctttaggga ggggtagtgg tgggagggaa
     2941 gaggatgtgt ctgtcctg
//
LOCUS       DAOMQG010000394         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13568_length_2956_cov_9.068942, whole genome shotgun sequence.
ACCESSION   DAOMQG010000394 DAOMQG010000000
VERSION     DAOMQG010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13568_length_2956_cov_9.068942"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLC06_15030"
     CDS             complement(<1..139)
                     /locus_tag="PLC06_15030"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRO91810.1"
                     /translation="MSNEQEIWDFLHQHLRSIFTRDADTYQATTSPDLSLYEWFVAPH
                     RQ"
     gene            275..1276
                     /locus_tag="PLC06_15035"
     CDS             275..1276
                     /locus_tag="PLC06_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRO91811.1"
                     /translation="MKKATIIFNPRAGQLNMTDKVDPVAAFWRAQGWRVNLWPTEAPG
                     HATTLARRAADEGEHLVLAAGGDGTMGQVANGLAHSETIMAPLPIGTSNALARDLKLP
                     RPQLLDSNAPIEASEALLAGRVQRVDLNHVQSQTSSGYALLWTGIGADGFLVQQIEPR
                     PTWSKRLGPIGYSIQMLSVLHRLPAMHAVVQVDDRIIEDDLLLIVISINRLYAGGLIA
                     LNTDALFDDGAFEVSLFSANEIAKRVGAPRAGLIARYLTELQLNMQESDPGVTCLAGK
                     RVVIETRPSMPCQTDGEWAGYTPLTCELIPRALRLLVPLSAPRSLFSKPGTSLADLL"
     gene            1389..2462
                     /gene="ltaE"
                     /locus_tag="PLC06_15040"
     CDS             1389..2462
                     /gene="ltaE"
                     /locus_tag="PLC06_15040"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRO91812.1"
                     /translation="MDKIDFRSDTVSWPTPAMREAMATAFVGDDVYGEDPTVNELEAL
                     AAARTGKEAGLFVASGTMGNLSAVLSHAGRGDEAILGADCHVISWEAGGMAALGGVMP
                     HPLPTDAYGRMDPAAVESAVRGDDPHLPRTRLVLVENSYGGKHGYPLSPDYFAQIAAV
                     ARRHDLAIHMDGARLFNAAVALNIDAADLTRDVDSITFCLSKGLCAPVGSVLCGSAEF
                     IHRARRARKILGGGMRQAGVLAAAGIVALNEMIERLADDHAHARLLAEGLARIPGVRV
                     NPEMVHTNMVFFDLADDVPLTPEDVTRGMRETANVWVGTNGPRGFRAVTHYWIDEAAV
                     ETFLGALEHILAQAPGASAQVHG"
     gene            2472..>2956
                     /locus_tag="PLC06_15045"
     CDS             2472..>2956
                     /locus_tag="PLC06_15045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRO91813.1"
                     /translation="MTSLVGQTLNKRYRLEALLGDGGMGTVYRSADLNLERQVAIKLM
                     HSHFARQEEFRARLVQEARTAAQLDHPSIVRVYDFGESELGLFIAMEYVDGGSLRDHL
                     RRLQRLQKYLPLIQCLQIGIQIAEALDYAHNRKIIHRDVKPGNIILKRLSRPDEAGAQ
                     P"
BASE COUNT          556 a          883 c          922 g          595 t
ORIGIN      
        1 cctgccggtg gggagccaca aaccactcat acaatgacag gtcagggctg gtggtagcct
       61 ggtaggtgtc ggcgtcgcgg gtgaagatgc tgcgcaaatg ttgatggaga aaatcccaaa
      121 tctcttgttc gttactcata actactcctg tggtgaagtg cggagtatat cactaaccaa
      181 ccgtgttcgt gctcttattt tacctggata tcgcgacgac atttgacagg aagtgtcgat
      241 atagcccaca attcgggctg atttcggcat actcatgaag aaagcgacca tcatttttaa
      301 cccgcgagcg ggtcaattga atatgaccga caaggtcgat ccggtagccg cgttctggcg
      361 cgcgcagggg tggcgcgtca acctgtggcc aacggaagcg ccggggcatg ccacaacttt
      421 ggcccggcga gcggccgatg agggtgagca tctggttttg gccgcgggtg gcgacggcac
      481 gatggggcag gtggccaacg ggttggccca tagcgaaacg attatggccc cgctgccgat
      541 cggcacgtcc aatgcgttgg ctcgcgatct gaagctgccc cgtcctcagc tgcttgattc
      601 caacgcaccc attgaggctt cggaagcctt gctggccggg cgcgtccagc gcgttgacct
      661 gaaccacgtc caaagccaga cctcatccgg ttatgcactc ctgtggacgg ggattggcgc
      721 ggatggcttc ctcgtccagc agatcgaacc acggccgacg tggtcgaagc ggctcggccc
      781 gatcggttac agcatccaga tgctgtcggt gctccatcgc ctgccggcca tgcacgctgt
      841 cgtgcaggtc gacgaccgga taatcgaaga tgatttgctg ctcattgtga tcagcatcaa
      901 tcgactctat gccgggggcc tcatcgcgct caataccgat gcgttattcg atgacggcgc
      961 gttcgaggtg tccttgttta gcgccaatga gatagccaag cgcgttggcg cgccacgggc
     1021 cggcctgata gcgcgctatc tgactgagtt acagctcaac atgcaagaga gtgaccccgg
     1081 agtaacatgc ctggccggga aacgcgtcgt catcgagacg cgcccctcga tgccctgcca
     1141 gacggacggt gagtgggccg gatatacgcc gctcacctgc gaactgatac cgagggcctt
     1201 gcggctgctc gtgccgcttt cggctccccg gtcgctattc agcaaacccg gcacgtctct
     1261 ggccgatctt ctttgaccgc gcttgcgcag cctcaacgca atttcatcgc caaattttta
     1321 acactcgacg aggcctagga ggcctgcccg tgcttgcaat gtggttgact tgacgtaaac
     1381 ttgcgctcat ggacaaaatt gacttccgtt ccgacaccgt tagttggccc acgcccgcca
     1441 tgcgcgaggc gatggcaacc gctttcgtcg gcgatgacgt ctacggcgaa gacccaacgg
     1501 tgaacgaact ggaggctctg gccgcggcac gcaccggcaa ggaagccggg ctgttcgtcg
     1561 ccagcggtac gatgggcaat ctgagcgccg ttctgtccca cgcggggcgc ggtgacgaag
     1621 cgattttggg agcggactgc catgtgatct catgggaagc gggcggcatg gcggcgctgg
     1681 gcggcgtgat gccccatccg ctgccgactg atgcttacgg ccgcatggac ccggccgcgg
     1741 ttgagagcgc cgttcgtggc gacgaccccc atttgccgcg aacccgtctg gttctggttg
     1801 agaacagcta cggcggcaaa catggctatc cgctatcgcc ggactatttc gcccaaattg
     1861 cagccgtggc tcgccgccac gatctggcta ttcatatgga cggtgcgcga ttgttcaacg
     1921 ccgccgtggc cctcaatatc gacgccgccg atctcactcg cgacgtggat tcgatcacgt
     1981 tttgcctgag caaggggctg tgcgcgccgg tcgggtcggt gttgtgcgga tcggccgagt
     2041 tcatccaccg ggcgcgacgg gcgcgcaaga ttctcggcgg cggcatgcgg caggcgggtg
     2101 tgctggccgc ggccggcatc gtcgccctca acgagatgat cgagcggctg gctgacgatc
     2161 acgcccatgc acgcctcctg gccgaagggc tggcccgcat tcctggcgta cgagtgaatc
     2221 cggagatggt tcacaccaat atggtcttct ttgatctggc tgacgacgtt ccattaacgc
     2281 cggaagatgt gacccgtggg atgcgcgaga cagccaatgt ctgggtcggc acgaacgggc
     2341 cgcgtggctt tcgagccgtc acccattact ggattgatga ggcggccgtg gaaacattcc
     2401 tgggcgcgtt ggaacacatt ctggcgcaag ccccgggtgc atctgcccag gtgcacggct
     2461 aggagagagc gatgaccagt cttgtcggtc aaaccctgaa taaacgctac cgcctcgaag
     2521 cgctgttggg cgacggcggg atgggtacgg tctatcgttc cgccgatctg aatctggagc
     2581 ggcaggttgc aattaagctg atgcatagcc atttcgcccg gcaagaggag tttcgcgccc
     2641 ggctggtgca ggaagcgcgc acggccgccc aactcgacca tccgtccatt gtccgcgtct
     2701 atgatttcgg cgagtctgaa ctggggttgt tcatcgccat ggagtacgtc gacggcggca
     2761 gcctgcgcga ccacctgcgc cgcttgcagc gactccaaaa gtatctgccc ctgatccagt
     2821 gcttgcagat cggcattcag atcgccgagg cgctcgacta tgcccacaat cgcaagatca
     2881 tccatcgcga tgtgaagccg ggcaatatca tcctgaagcg cctcagccgc ccggatgaag
     2941 ccggcgcgca accgtt
//
LOCUS       DAOMQG010000395         2954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13579_length_2954_cov_9.173853, whole genome shotgun sequence.
ACCESSION   DAOMQG010000395 DAOMQG010000000
VERSION     DAOMQG010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13579_length_2954_cov_9.173853"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLC06_15050"
     CDS             complement(<1..584)
                     /locus_tag="PLC06_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRO91814.1"
                     /translation="MQDIMPYRSTEKTRLKKDAKRTAMMQAAVRVFADKGYHAATVRD
                     IVNEADVAIGTFYFYFPDKETLFVHLFEETAAFLLQAIEQAINSRPSLPAQLEAAIQA
                     YINVAFYEPAVVQLLLVGGVGSVPSLAERRAEYREKLIRVWQRPLMAALEQKKIEPQN
                     VRRTAEALVGGFDEIIYHLLTAAGTEREGQQAVG"
     gene            617..2056
                     /locus_tag="PLC06_15055"
     CDS             617..2056
                     /locus_tag="PLC06_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRO91815.1"
                     /translation="MVRITIDRNSPETGYKQVYRRLREAILTGELVSGARLPSTRILA
                     GEAGVARVTISRAYEQLAAEGFVESRVGAGTYVAAKIPTIAEAKATEFKPVLTNWGQR
                     AIALTRQAERGGDGGRPEIDFGFGRSFAERFPYDVWRRLLGRYLSTDDAMLSRYGSAA
                     GFYPLRQAVADYLVRARGVRCTPEDVVIVSGAQQAIDILARLLINAGDEVLIETPGYR
                     DAFALFRLHQARLVGLSVDNQGLPVDDIPLGCQAKLAFVTPSHQFPKGGTMPLVRRLA
                     LLAWARKHSAIIIEDDYDGDLRYEGDSLSALEGLDEDGRVIYLGTFSKVLFPALRLGY
                     LVLPRTLVAPFVRAKELVDRGAPTLTQAAVADFLSEGHFERHLRHLRQLYGEKRVALV
                     TALDEYLGDRVRFSPVAAGLHVMIYANTPIDEAGLAHSAAALGVRVYGGAPYHLERPA
                     PPSFLLGFSGLSESEIVEGVRRLAAAWPE"
     gene            complement(2063..2581)
                     /locus_tag="PLC06_15060"
     CDS             complement(2063..2581)
                     /locus_tag="PLC06_15060"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRO91816.1"
                     /translation="MVTIGKLENTPVGRIWVAASDDGLVGVNLWDEPERFLIGIARMT
                     SEIPDPNAQPNAIVRATIAELEAYLHGELREFTIPIEWAILKPFHREVLEQVMAVPYG
                     HTTSYGAIAQRIGRPGAVRAVGRANATNPIPIIIPCHRILGADGKLHGYGARGGLETK
                     AWLLRLEGSWLI"
     gene            complement(2517..>2954)
                     /locus_tag="PLC06_15065"
     CDS             complement(2517..>2954)
                     /locus_tag="PLC06_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRO91817.1"
                     /translation="HPRLAKAALGANETIPWAYFPNGIDAALTLKAEGYRLWALERLT
                     TAEPSTPPVPDSPAKIALIVGNEKAGIDPGILDLCDAVLGLPMAGHKSSLNAAVAFGI
                     ALYWLRYGKIIQPTVNRPAGAQHGNDRQAGKHTGGEDLGSRVR"
BASE COUNT          494 a          843 c          975 g          642 t
ORIGIN      
        1 tcgccgacgg cctgttgtcc ctcgcgttcg gtgccggccg cggtgagcag gtgatagatg
       61 atctcgtcaa atccgccgac caatgcctcg gcggttcgac gtacattttg cggctcaatt
      121 ttcttttgct ccagcgcggc catgaggggg cgctgccaga cgcggatcag cttctcgcgg
      181 tattcggccc ggcgctcggc cagcgaaggc accgatccca cgcccccaac gagcaacaac
      241 tggactaccg ccggctcata gaaagcgacg ttgatgtagg cttgaatggc cgcctcaagc
      301 tgcgccggga gcgaagggcg actgtttatc gcttgttcga tggcctggag caaaaaggcg
      361 gccgtctcct caaaaagatg gacaaacagg gtttccttgt cggggaagta gaagtagaac
      421 gtcccgatgg cgacgtcggc ctcattgacg atgtcgcgca cggtggccgc gtggtatcct
      481 ttatcggcga acacgcgcac ggcggcctgc atcattgccg ttcgcttggc gtccttcttg
      541 aggcgggtct tttctgtgct gcgatagggc ataatatcct gcatctatca tacttaaatg
      601 tcaatggagt cgcaatgtgg ttcgcataac catagatcgt aatagccccg aaaccggcta
      661 caaacaggtg tatcgtcggc ttcgtgaggc gatcctgacc ggagagcttg tctcgggcgc
      721 gcgcttgcct tcaacccgaa ttttggccgg tgaggccggg gtggcgcgcg tcacaatctc
      781 gcgcgcctac gagcaactgg cggccgaggg gtttgttgag agtcgcgtcg gggcgggaac
      841 ttacgtcgcc gcgaagatac ccactattgc agaagcaaag gcgacggaat tcaagccggt
      901 gcttacgaat tggggccagc gggcaatcgc cctgacccgg caggctgagc gaggcggcga
      961 tggcggccgc ccggagatcg attttggttt cggccgctcc ttcgccgaac gttttcccta
     1021 tgacgtatgg cgacggcttc tgggccgcta tctttccacg gatgacgcga tgttgtcacg
     1081 atacggttcg gcggccggtt tctatccgct gcgccaggct gtggctgatt acctggtgcg
     1141 ggcacgcggc gtgcgctgca cgcctgagga tgtggtcatt gtgagcggcg cgcaacaggc
     1201 catcgacata ctggcgcgac tgctgatcaa tgccggggat gaggtgttga tcgagacgcc
     1261 gggttaccgc gacgcctttg ccctgtttcg gctccatcag gccaggcttg tgggactgtc
     1321 ggtcgataat cagggcctgc cggtggatga cattccgctt ggttgtcagg caaagttggc
     1381 gtttgtgacg ccgtcgcatc aattccccaa ggggggaacc atgccgctgg tgcggcggct
     1441 ggcgctgctg gcctgggcgc ggaagcattc ggccatcatc attgaggatg attacgatgg
     1501 cgatctgcgc tatgaaggtg actcgctttc cgcgctcgag gggctggatg aagatggtcg
     1561 cgtcatttat ctggggacgt tttcgaaggt gctgtttccc gcgttgcggt tgggttatct
     1621 cgtgctgccc cgaacgttgg tagcgccgtt tgtccgggcc aaggagctgg tcgatcgtgg
     1681 cgcgccgacg ctgacccagg cggccgtggc cgactttctg tccgaaggtc acttcgagag
     1741 gcacctgcgc catctgcgcc agttgtatgg cgaaaagcgg gtcgcgctcg ttactgcgct
     1801 ggatgagtat ttgggcgacc gcgttcgctt ttcccccgtt gcggccggac tgcacgtgat
     1861 gatttacgcg aatacgccta ttgatgaagc cgggcttgcc catagcgcgg ctgcgttggg
     1921 ggtacgggtc tatggcggcg cgccttatca tctggagcgg ccggctcccc cgtcatttct
     1981 gctgggattt agcgggttga gtgagagcga gatcgtcgag ggggtgcgcc gtttggcggc
     2041 tgcctggccg gagtagcgca actcagatca gccaactgcc ctcaagcctg agaagccatg
     2101 cctttgtctc caggccgccc ctggctccat agccgtggag cttgccgtcc gcgccgagga
     2161 tgcgatggca gggaatgatg atggggatgg ggttggtcgc gtttgcccga cccaccgccc
     2221 gcactgcccc cggtcggccg atgcgctggg cgatggctcc atagctcgtc gtgtggccat
     2281 agggcaccgc catcacctgc tccagcacct cgcgatggaa aggcttgaga atggcccatt
     2341 caatcgggat ggtgaattcg cgaagctcgc cgtgcaaata ggcctcaagc tcggcgatag
     2401 tcgctctgac gattgcgttg ggttgagcgt tcggatcggg gatctcgctc gtcatgcgcg
     2461 cgatgccgat caggaatcgt tccggctcgt cccacaggtt gacacccacc agcccatcat
     2521 ctgacgcggc tacccagatc ctccccaccg gtgtgttttc cagcttgccg atcgttacca
     2581 tgttgtgctc ccgccggtcg atttaccgtt ggttggatta ttttaccata tcgtagccag
     2641 tagagagcaa tgccgaaggc gacggccgcg ttgagcgatg atttgtgccc ggccatcggc
     2701 aggcccagga cggcgtcgca caaatcgagg ataccggggt cgatgccggc cttttcgttg
     2761 ccgacgatca gggcgatttt ggccggggag tcggggacgg ggggagtcga gggttccgcc
     2821 gtcgtcaggc gttccagcgc ccacagccga tagccttcgg ccttgagcgt tagggccgca
     2881 tcaatgccgt tggggaaata ggcccagggg atggtctcgt ttgcgccaag agccgctttg
     2941 gccagcctgg gatg
//
LOCUS       DAOMQG010000396         2945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13636_length_2945_cov_11.140138, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000396 DAOMQG010000000
VERSION     DAOMQG010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13636_length_2945_cov_11.140138"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..307)
                     /locus_tag="PLC06_15070"
     CDS             complement(41..307)
                     /locus_tag="PLC06_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91818.1"
                     /translation="MFINYSHAKRLRHIAAGRPRTICHIFAPNNRRGPDAYETSHLIL
                     GTYGGDPDARPQVTIAGVDPPGEERVLIDYAELIEVVAYPPSAP"
     gene            complement(371..1666)
                     /locus_tag="PLC06_15075"
     CDS             complement(371..1666)
                     /locus_tag="PLC06_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91819.1"
                     /translation="MPPSPSPDIYPPLPTATYTLPPYPWPTATPGPTEEPTETEEPTP
                     TDPPVPTLPPTPVVTLIPTVAAPFISFPEGTTPQPFTFYYRDGDVILSLSSESGAEPQ
                     VFLNPLAELGLYLPPRPEYISDWGAMSPDGRTLALVLTNDPVSKMPEGATDAFSAAPH
                     PVSIYLLDMAGRELRLLVPEGFIPVWSPDGSRIAYRSTKTLGLWVVDVITGEAREVYG
                     VSDEHFVRGFTWASDNRHLSLIDDKLYETTESLIVDANGLEPPRAIVPGRTYGVSTAE
                     WSPVDQRLVFTMTGPGRQPVQDLWLMNSDGTAARQLTHDLNVLGGRPQWSPDGRWIAF
                     SALAAYESEYSSYDLWLSDPTDGVLQRLTYDWDPGPYDPSYDPSIQDSQPQWSPDGIQ
                     LLFFKSSNQVWLLSLIDGRRRQLLESEDNLYDSGLLVTR"
     gene            2056..>2945
                     /gene="dnaX"
                     /locus_tag="PLC06_15080"
     CDS             2056..>2945
                     /gene="dnaX"
                     /locus_tag="PLC06_15080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRO91820.1"
                     /translation="MSQALYRKWRPARFDQVIGQEHVTHTLQAGVAAGRVGHAYLFCG
                     PRGTGKTTMARLLAKAVNCTAEDPAERPDDSCPLCLAVDEGRFLDLIEIDAASNTGVD
                     DIRNLRDKVNFAPSQGTYKVYIIDEVHMLSTAAFNALLKTLEEPPAHTIFVLATTEEH
                     KVPLTIKSRCQQFNFRLLTTPEISARLNWLAAQEGLAIEPAAVELIARQGAGSLRDAE
                     SLLDQLVVSPDDRITLEQAQIVLGTAPDAAVLAVTDAWLDGDSARGLTAIHDALGSGA
                     DPRQFCRQMVAHLRQLLMLQA"
BASE COUNT          614 a          910 c          891 g          530 t
ORIGIN      
        1 gtaaatatga gcgagcaaca tcgactatcc gccgctaccc tcaaggcgcg gagggtgggt
       61 aagcaaccac ttctatcagt tcagcatagt cgatgagaac cctttcctcg ccgggcggat
      121 cgacgcccgc aattgtgacc tgcggccgcg cgtcgggatc accgccatac gtgcccagaa
      181 tgaggtggct ggtttcgtag gcgtctgggc cgcgacgatt gttaggggcg aagatgtgac
      241 agattgttcg tggccggccg gcggcgatat ggcgcagccg cttagcgtga gaatagttga
      301 tgaacacagg ccaagcctta tgagcataag ctatttcctc ctatccatgg cccgttaccc
      361 ttccaataac tcaacgtgtg acaaggagcc cgctgtcgta cagattgtct tcgctttcca
      421 gtaactggcg gcgacggcca tcgatcaacg ataaaagcca tacctgattc gaggatttga
      481 aaaagaggag ttgtatccca tcgggagacc attgcggttg agagtcctgg atcgaagggt
      541 cgtatgaagg gtcatatggc cccgggtccc agtcgtaagt caaccgctgg agcaccccat
      601 ctgtggggtc gctgagccac aggtcatacg atgaatactc ggattcatag gccgctaaag
      661 cgctaaacgc aatccagcgg ccgtccggcg accattgcgg ccgtccgccg agcacgttca
      721 ggtcatgggt caattgccgc gcggccgtgc cgtcactgtt catgagccac aggtcctgaa
      781 caggttgccg cccgggaccg gtcatggtaa agaccagtcg ttgatccacc ggcgaccatt
      841 cggcagtgga aacgccgtaa gtccgcccgg gaacaatggc cctcggcggc tccagaccat
      901 tggcatcgac aatcaacgac tccgttgttt catacaactt gtcatctatg agcgacaagt
      961 gacgattgtc ggatgcccag gtaaacccgc gcacaaagtg ctcatcgctg acgccgtaca
     1021 cttccctggc ttcgcctgtt atgacatcga ccacccagag acccagggtc ttcgtggaac
     1081 gataagcgat ccggctgccg tcgggcgacc agacagggat aaaaccttcc gggacaagga
     1141 gccgcaattc tcgacctgcc atatccagaa gatagatact caccggatgc ggcgcggccg
     1201 agaacgcatc ggtcgctcct tcgggcattt tggaaacggg atcattcgtc agcaccaggg
     1261 cgagtgttcg cccatcaggc gacatggcgc cccaatcaga gatgtattcc ggtcgcggcg
     1321 gcaagtagag gcccaactcg gccaggggat tgagaaaaac ctgcggctcc gcgcccgact
     1381 cactgctcag cgagagaatg acgtcgccat ctcgatagta gaaggtgaac ggctgaggtg
     1441 tcgtcccttc gggaaatgaa atgaagggcg ctgcgacagt cggaatgagg gtgacaaccg
     1501 gcgttggcgg cagcgtcggc acgggcgggt cggtcggcgt cggctcctcg gtctctgtgg
     1561 gttcctcagt cggccccggc gtcgctgtcg gccacgggta gggcggtagt gtgtaggttg
     1621 cagtaggtaa cggcgggtaa atgtctgggg aaggtgaagg gggcaaggtt gcgatttgcg
     1681 caagtatcga cgttggtgta ccgccgggta tgtcaccttg cgggaatgaa gctgttggtt
     1741 ccggtatggc ggtcggcacg caagcggtgc aagctaacaa gaccaacaga aaaatcggcc
     1801 gccgacaaaa caggttacgc gctgcaaaca tgttaatgct cccggcgctg caaacacggt
     1861 acgccccaac tctcgaaaac cagcgacaac agcatcataa cagatgcagg gcagggcgcg
     1921 caagcgtcgt tatgcctacc cattcatgcg gcggcccgcc ttgttccccc cccttatcgg
     1981 tgttaaactt gcgccatgca aacctcagag gtcttctgag acctctgagg tttcagaggt
     2041 ttcacgcgaa agctcatgtc gcaagcactc taccgcaaat ggcgaccggc ccggttcgac
     2101 caggtcatcg gccaggaaca cgtcacccac acgctgcaag ccggcgtcgc tgccgggcgc
     2161 gtgggccatg cctacctgtt ttgcgggccg cgcggcacgg gcaagacgac gatggcccga
     2221 ctgctggcca aggcggtcaa ctgcacggcc gaagacccgg ctgaacgccc cgacgatagc
     2281 tgccccctct gtctggccgt cgacgagggc cgctttctcg acctgatcga gatcgacgcc
     2341 gccagcaata ccggcgtcga tgacattcgc aacctgcgcg acaaggtcaa cttcgccccc
     2401 agccagggca cgtacaaggt ctacatcatc gacgaagttc acatgctctc cacggccgcc
     2461 ttcaacgccc tgctgaagac gctggaggag ccgccggccc acaccatctt cgtgctggcg
     2521 acgaccgaag agcacaaggt cccgctgacg atcaaatccc gctgccagca gttcaacttc
     2581 cggctgctga ccacgcccga aatctccgcc cggctcaact ggctggcggc ccaggagggg
     2641 ctggcgattg aaccggcggc cgtcgaactt atcgcccggc agggcgcagg cagcctgcgc
     2701 gacgccgaga gtctgctcga ccaactggtt gtctcgcccg acgaccgcat cacgctggag
     2761 caggcgcaga tcgtgctggg cacggccccc gacgcggccg tgctggccgt cactgacgcc
     2821 tggctcgacg gcgacagcgc ccgcggcctg accgccatcc atgacgccct cggctccgga
     2881 gccgacccgc gccagttctg ccgccagatg gtggcccacc tgcggcagtt gctgatgctc
     2941 caggc
//
LOCUS       DAOMQG010000397         2943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13647_length_2943_cov_9.344529, whole genome shotgun sequence.
ACCESSION   DAOMQG010000397 DAOMQG010000000
VERSION     DAOMQG010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13647_length_2943_cov_9.344529"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLC06_15085"
     CDS             <1..245
                     /locus_tag="PLC06_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91821.1"
                     /translation="FSNIGYKNGALAGVLNSAYYAYRWGDGAPVIVVLFFRDITQQTY
                     RSWRFDLPHDELARWLLADPAAIPALAAALRDSAAP"
     gene            336..1328
                     /locus_tag="PLC06_15090"
     CDS             336..1328
                     /locus_tag="PLC06_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91822.1"
                     /translation="MSDELPPTPQSEQVEAPIQIARRSGSGSAILVILLATLPLIGIM
                     YLGHQLLNLPFVPFDLYDWPIRAGIAPWISLIDTLTGSQAAVGGNIAQSASLVQWLLS
                     LGLFLLVALIVGLAFRTFVTRRDRHPDLIDGVTIGILFAAPMIFMSLTTSGSPLPALL
                     NVVWLGALFVVWGIVLAYAFGHLMEPAGATLPDGSLAGGIGRRQFLLQFSAGAAAITA
                     ISAAGAALAPGKDAAQLQKSLPMVAPDFLQAQQELFGNFRRFVIVRGGTTSAAESNVL
                     ALGAEYPDRNYVSVWLGGRSPIVIYENIQTALSAFSTEEQTAGIYWLDDQSSGN"
     gene            1416..1850
                     /locus_tag="PLC06_15095"
     CDS             1416..1850
                     /locus_tag="PLC06_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91823.1"
                     /translation="MTSHTNGTQSPISPDLLEILRDPQAIQAGDQFGTDPGRLELVRD
                     SWLVSADTGYKYPIRDGIPVMLIEEGARWKDTPVDALPVPPPAAEPAPVTRLSEPAMP
                     AAQSGPDYRILLAAAAVLLFLLAVISLGRKRPGGHSETPPAA"
     gene            1847..>2943
                     /locus_tag="PLC06_15100"
     CDS             1847..>2943
                     /locus_tag="PLC06_15100"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRO91824.1"
                     /translation="MTSRLTDSRLANIGARTIQTAYVTLHDQFQAITERASVRFDAQD
                     WAGMQADAAERLYLYRRIVDLVEAAIRDLLGDRAENKLIWASMKAVYSGLIADRDDWE
                     LAETFFNSATRRIFTTVGVDHQIEFVATDFETPPTRPRSPVYRSYGHSSSTAGLVAIV
                     LRDNSFTTPYANLQRDAELVAQRIDAQLEKLGALQSVDRLELIRSPFFRGMGAYLVGR
                     LFSGSHRIPLALALIHTPNGLIVDSVLLDENSVSILFSFAHSYFHIAVDRPYDLVYFL
                     KSIMPRKRTGELYISLGYNKHGKTELYRDLLDHLAYTTEKFEVARGQRGMVMMVFTMP
                     GYDMVFKLIKDFFNYPKDSTRKQVKGKYDLV"
BASE COUNT          535 a          986 c          816 g          606 t
ORIGIN      
        1 tattcagcaa catcggctac aagaatgggg cgctggcggg cgtgctcaac tccgcttact
       61 atgcctatcg ctggggcgac ggcgcgccgg tgatcgtcgt tctgttcttt cgcgacatca
      121 ctcagcagac ctatcgctca tggcggttcg acctgccaca cgacgagctg gctcgttggc
      181 tgctggccga tccggcagct atccccgcgc tggccgcggc gttgcgcgat tcggccgccc
      241 cctgatcttg ccaaagctac cgttgcatga gaagatgatg ccgtcgcgcc tgccttcagg
      301 ataatcgtaa ccgtgcccat ttatacagga taactatgtc tgatgaactc ccacccacgc
      361 cgcaatctga gcaggtcgaa gctcccatcc aaatcgcgcg tcgttccggc tcaggatcgg
      421 cgatcctggt cattcttctg gccacattgc ccctgatcgg gatcatgtat ctcggtcatc
      481 aattgctgaa cttgccgttc gtccccttcg atctgtacga ttggcccatt cgggcaggga
      541 tcgcgccgtg gatcagcctg atcgatacgc tgacaggctc ccaggcggcc gtcggcggca
      601 acatcgccca gagcgcgtcg ctagtgcaat ggctcctgtc gctggggctg tttctgcttg
      661 ttgctctcat cgtgggcctc gcgttccgca ccttcgtcac gcggcgcgat cgccatcccg
      721 atctcatcga cggcgtcact atcggcattc tcttcgccgc gccgatgatt ttcatgagcc
      781 tgacgacatc cggctcaccg ctgcccgccc tgctcaacgt cgtctggctt ggcgctctgt
      841 tcgtcgtatg gggcatcgtc ctcgcctatg cattcggcca cctgatggaa ccggccggag
      901 caacgctgcc cgacgggtca ctggcggggg gcattgggcg acgccagttc ctgttgcagt
      961 ttagcgcggg cgcggcggcc atcaccgcca ttagcgctgc cggcgcggca ctggccccag
     1021 gcaaggacgc cgcgcaactg caaaagtcgc tacctatggt cgccccggac tttctgcaag
     1081 cgcaacagga gttgttcggc aatttccgac gcttcgtcat cgtgcgcggc ggaaccacat
     1141 cggccgccga atcaaacgtt ctcgcccttg gagccgaata tcccgatcgc aattatgtct
     1201 ccgtctggct cggcggccgc agccccatcg tcatctacga aaacatccag accgccctct
     1261 ccgccttcag caccgaggag cagactgcgg gtatctactg gctcgacgac cagtcttccg
     1321 gcaattgaca gagcctcggc ggaagtcgta tcattcatat caggaaatcg ggtctcaccc
     1381 acaaggcccc gatttcatca ctacggagta acagcatgac atctcatacc aacggcacgc
     1441 aatcacccat cagcccggac cttcttgaga ttcttcgtga tccccaggct attcaggcgg
     1501 gtgatcaatt cggaaccgat cccggccggc tggaactggt ccgtgattcc tggctggtct
     1561 cggccgatac cggctacaaa taccccatcc gtgacggcat tcccgtgatg ctcatcgaag
     1621 aaggcgcgcg ctggaaggac accccggtag acgctctgcc cgtacccccg cccgcggccg
     1681 aacccgcccc cgtcacccgt ctcagcgagc ctgcaatgcc cgctgcccag tcggggccgg
     1741 attatcgcat cttgctcgcc gccgcggccg ttttgctctt cctgttggct gtcataagcc
     1801 tgggacgcaa gcgtccgggt gggcattcgg agacgccgcc ggccgcatga cctcacgcct
     1861 gacggacagc cggctggcca atatcggtgc cagaacgatc cagaccgcat acgtcacgct
     1921 ccacgaccag ttccaggcca tcaccgagcg cgcaagcgta cgcttcgatg cccaggattg
     1981 ggcgggaatg caggcagacg cggccgaacg cctctacctt tatcgtcgta tcgtcgatct
     2041 cgtcgaggcg gccatacgcg acctgctggg cgaccgggcc gagaacaagc tcatatgggc
     2101 cagtatgaag gccgtctact cggggcttat cgccgatcgg gatgattggg agctggccga
     2161 aacattcttc aactcggcga cgcgccgcat cttcaccacg gtgggggtcg atcatcagat
     2221 cgaattcgtg gccactgatt tcgagacccc acccacgcgg ccgcgctcgc ccgtctatcg
     2281 ctcctacggt cactcgtcat caacggccgg attggtagcc atcgtcctgc gcgataacag
     2341 cttcacgaca ccctatgcca accttcagcg cgacgccgag ctggtagcgc agcgcatcga
     2401 cgcccaactg gagaagctgg gcgcgttgca gtcggtcgac cggctggagc tgatccgctc
     2461 gccattcttt cgcgggatgg gcgcgtatct ggtcggtcgc ctcttcagtg gctcgcatcg
     2521 catccccctg gctctggcgc tcatccacac gccgaatggg ctgatcgtcg actcggtgct
     2581 gctggacgaa aacagcgtca gcatcctgtt cagcttcgcc cattcctact tccacatcgc
     2641 cgtcgatcga ccctacgatc tcgtttactt cctgaagagc atcatgcccc gcaagcgaac
     2701 gggcgagctt tacatctcgc tcggctataa caagcacggc aagacggagc tttatcgcga
     2761 cttgcttgac catctcgcct atacgacaga gaagttcgag gtcgcccgtg gccagcgcgg
     2821 catggtcatg atggtcttca cgatgcccgg ctatgacatg gtgttcaagc tcatcaagga
     2881 cttcttcaac tatccgaagg actccacgcg caagcaggtc aagggcaaat acgatctggt
     2941 cta
//
LOCUS       DAOMQG010000398         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13686_length_2937_cov_7.699167, whole genome shotgun sequence.
ACCESSION   DAOMQG010000398 DAOMQG010000000
VERSION     DAOMQG010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13686_length_2937_cov_7.699167"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="PLC06_15105"
     CDS             <1..1499
                     /locus_tag="PLC06_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRO91825.1"
                     /translation="VDRWDYRVAVSPSVLTDPEALADSLAPILQIRRSELLYDMESPN
                     MYVVLAPRVTADVADAIRELEYGDEIQLDPLPRRFYPQDKLMCHVLGFVNFDNTGSAG
                     VEGEYQKELAGEEASATVPISPLKEQGSVIAREGSDIVLTIDRSVQYVVERHLKEALA
                     EHGAVSGSIIVMDPRTGEILAMAAEPCYSPNEFYDLKEGEDFNPLVSAVYEPGSVMKL
                     ITMAAALDSGTVTPTTTYNDTGAIEVGGHISYNWDRGAHGVVDMTTLLARSLNVGAAT
                     IATWMGPTTFYDYLQRFGFGHSTNIDIANEAEGLMPLPGSDLWQESFIATNAYGQALA
                     VTSIQMISAISALANDGRLMQPHVVSEIRDEFGTRHIEPIMVGQIISENTAAQMTAMA
                     TVAVQQEVQEALVEGYTVAGKTGTAQIAEAFGYHPTDVIGSFVGWLPADDPQIVVYVK
                     LDRPQSAPWGSMTAAPVFSKLAKELVVLLDIPPDAVRLQAGNPTGGGG"
     gene            1507..2928
                     /gene="murF"
                     /locus_tag="PLC06_15110"
     CDS             1507..2928
                     /gene="murF"
                     /locus_tag="PLC06_15110"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRO91826.1"
                     /translation="MTPRSGPTLGHILEALTSYRPTHEEPAVSSFVVDSREAIRDSVF
                     IAFPGEKVDGHDYVADAFANGAMAALVQHPPAGDWAILDARSGEVELPDRLPVCILVD
                     DTLRALQQIARNWRAKFDIRVIGITGSVGKTSTKELAAAVLSQRYRTLKSEGNQNNEI
                     GVPLTLMNLRAYHERAVIEMGMYAKGEIALLCDIARPTVGVITIIGPVHLERLGSIEA
                     IVDAKQELVAALPRDGVAILNMDDPRVMGMAAHTEARIFTYGLDSGADLWADQIESMG
                     LDGVRFTMHHGDESMNVRVPLLGRHSVHTALRAAAIGLIEGLSWEEIIPGLGAMSSQL
                     RLVVIPGPRGSLLIDDTYNSSPDSALAALNLLADLDGRHIAVLGDMLELGEAELQAHQ
                     IVGRRVADVADLLISVGPRAQTIAYQAIAAGLPADRVSIVDDAPAAVPVLEEIINAGD
                     VILIKGSLDMAMDRIVTALGRLD"
BASE COUNT          569 a          885 c          919 g          564 t
ORIGIN      
        1 ccgtcgatcg ctgggattat cgtgtcgccg tgtcgcccag cgtcctgacc gacccggaag
       61 cgttggcaga ttcgctggct cccattctgc agattcgccg cagcgaactg ctctatgata
      121 tggaatcgcc gaacatgtat gttgtgctgg cgccgcgcgt cacggccgac gtagccgacg
      181 ccatccgcga actggaatat ggcgatgaga ttcagcttga ccccctgccc cgccggttct
      241 atccgcaaga taaactgatg tgccacgtcc tgggctttgt caatttcgac aacacgggca
      301 gtgcgggcgt cgagggcgag tatcagaagg aactggccgg cgaagaggcc agcgccacag
      361 tgccgatctc ccccctgaaa gaacagggct ccgtcatcgc tcgcgaggga tccgatatcg
      421 ttttgacgat cgaccgcagc gtgcagtacg ttgtcgagcg tcatcttaaa gaagcgttgg
      481 cagagcatgg cgcggtcagc ggttcgatca tcgtcatgga cccacgcacg ggtgaaattc
      541 tggccatggc cgccgagccg tgctattcgc ccaatgagtt ctacgatctg aaagaaggcg
      601 aagattttaa cccgctggtc agcgccgtct acgagccggg ttcggtcatg aagctcatca
      661 ccatggccgc cgcgctcgat tccggcactg tgacgccgac gacgacctat aacgataccg
      721 gtgcgatcga agtgggcggc cacatctcct ataactggga tcgtggcgct catggcgtgg
      781 tcgatatgac caccctgctg gcgcgctcgc tcaacgtcgg cgcggcgacc atcgccacgt
      841 ggatggggcc gacgacattc tacgactacc tgcagcgctt tggcttcggg cactcgacca
      901 acatcgatat cgccaatgaa gccgagggcc tgatgccgct gcccggcagc gatttatggc
      961 aggaatcatt catcgccacc aacgcctatg ggcaggctct ggccgttact tcgatacaga
     1021 tgatctcggc catctcggcc ctggccaatg acggccggct catgcagccg catgtcgtgt
     1081 ccgagatcag ggatgagttc ggcacacgcc atatcgagcc gatcatggtg gggcagatta
     1141 tcagcgagaa cacggcggcg cagatgacgg ccatggcgac cgttgccgta caacaagagg
     1201 tgcaggaagc gttggtcgaa ggctatacag tcgccggcaa gacgggcacg gcccagatcg
     1261 ccgaagcatt tggctatcac ccaaccgacg tgatcggttc cttcgtcggc tggctgccgg
     1321 ccgacgatcc acagatagtc gtttatgtca agctagatcg gccacagagc gccccctggg
     1381 gatcgatgac ggccgcgccg gtcttctcga agttggccaa agagttggtg gttctgctcg
     1441 atattccgcc ggacgcagtt cgtttgcagg cgggcaaccc gaccggaggt ggtggttaac
     1501 gctgttatga cgccccgctc aggccccacg ctgggccaca ttctggaagc gctgacgagt
     1561 tatcggccga cccacgagga accggccgtt tcgtcttttg tggtggatag ccgcgaagca
     1621 atccgcgaca gcgttttcat tgcgtttccg ggcgagaagg ttgacggcca cgattacgtt
     1681 gccgacgcct tcgctaatgg ggccatggcc gcgctcgtcc agcacccacc ggccggtgat
     1741 tgggcgatcc ttgacgcgcg cagcggggaa gttgaactcc cggatcgcct gccggtgtgt
     1801 atcctggtcg acgataccct gcgcgccctg cagcaaatcg cgcgcaactg gcgggccaaa
     1861 ttcgatattc gggtcatcgg catcaccggc agtgtcggca agacctcaac caaggaactg
     1921 gccgccgcgg tgctgtcaca gcgctatcgg acgttgaaat cagaaggcaa tcaaaataac
     1981 gagatcggcg tcccgctgac cctgatgaac ctgcgcgcct atcatgagcg ggccgttatc
     2041 gagatgggca tgtacgcgaa gggcgagata gccctgctat gcgacatcgc ccgacccact
     2101 gtcggcgtca tcaccatcat tggcccggtc catctggaac gattgggatc gatagaggcc
     2161 atcgtggacg ccaagcagga actggtcgcc gccttgccca gggatggcgt agccattctg
     2221 aacatggatg atccgcgagt gatgggcatg gcagcgcaca ctgaggcgcg catcttcacc
     2281 tatgggctgg atagcggggc ggatctatgg gccgaccaga tcgagtcgat gggactggat
     2341 ggcgtgcgct tcacgatgca ccatggcgac gaatccatga acgtgcgcgt acccctgctc
     2401 ggacgtcaca gcgtccacac cgccctgcgc gcggccgcca taggcctgat tgaggggcta
     2461 agttgggaag agattatccc cggcctgggc gcgatgtcgt cgcaactgcg actcgtcgtg
     2521 attcccggcc cgcgtggttc gttgctcatc gacgacacct ataattccag tcccgactcg
     2581 gcgctggcgg ccctgaacct attggccgat ctggatggtc gtcacatcgc cgttctgggc
     2641 gatatgctgg agctgggcga ggccgagcta caggcacacc agatcgtcgg ccggcgcgtg
     2701 gccgatgtgg ccgatctgct gatcagtgtc ggcccgcgcg cgcagacaat cgcctatcag
     2761 gccatcgccg ccggcctgcc cgccgatcga gtgagcatcg tcgatgacgc accggcagcg
     2821 gttccggtgc tggaagaaat catcaacgcc ggcgatgtga tcctgattaa ggggtcgctg
     2881 gacatggcga tggatcgcat cgtcaccgct ttagggaggc tggactaatg ggctggg
//
LOCUS       DAOMQG010000399         2931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13724_length_2931_cov_9.982267, whole genome shotgun sequence.
ACCESSION   DAOMQG010000399 DAOMQG010000000
VERSION     DAOMQG010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13724_length_2931_cov_9.982267"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..769
                     /locus_tag="PLC06_15115"
     CDS             41..769
                     /locus_tag="PLC06_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRO91827.1"
                     /translation="MWRFRISRLVFTLVQNILCRLVVAGRENIPAEGPYILAVNHLST
                     ADIGIIFVGFPAQEWRYFAGEKWADHPLWGPLMSWLGVIFINRGEVDRRALREALVAL
                     EEGAIFALAPEGTRSKTGALQPGKDGSAFLASQAGVPILPVGISNSDVLFADFRRLRR
                     ATITMRIGRPFSLPDLGRRPRGRDLSDFTCLIMTHIAALIEPRHRGAYGDTPALAALL
                     AGDDPWPACAGASVMSEEPEQSAP"
     gene            979..>2931
                     /locus_tag="PLC06_15120"
     CDS             979..>2931
                     /locus_tag="PLC06_15120"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRO91828.1"
                     /translation="MSSAVTPAVESSTLTLPENSMAVLRRRYLRRGPDGQPIETVEEM
                     FRRVAHHVAAVEADHGGDTIAAENAFYNLMTELRFFPNSPTFTGAGTPLGQLAACFVL
                     PIADDMGRDPAGIFQTLRDAALIQQTGGGNGFAFSRLRPKDDHVKTSAGKATGPVGFL
                     RVYDQAFGEIAQGGTRRGANMAVLRVDHPDIFEFISCKASEYAITNFNISVGVTDKFV
                     QAVKDDGDFDLINPRDGKVWRTVRARDIFDEIVKYAHRNGEPGVLFLDAANRSNPVPH
                     LYDLEATNPCGEQWLGPYENCCLGSINLGQHVTEDGKIDWELLRRSTEESTHFLDNVV
                     TANAYVPAVPQLRVAALNARRIGLGIMGLGDLFYRTGVRYGSEEGQALAGQLFEFIRY
                     HSMRTSMELARERGAFLAIEGSIYDPADFKWSPPRPLVKGRKDYGRPAVDWKGLVADI
                     KAHGIRNAAQTTVAPTGTIATVAGIEGYGCEPVFALAYIRHVNDNGKDLQLQYTSPLF
                     EQALLKAGLDEATRDEIIAEVNRVGSCQHIDRLPEAIRNTFVVSSDIAPEEHVRMQAA
                     IQAFVDNSISKTCNFPAGATAEDVAAVYMMAWELGCKGLTVYVTGSRETVTLETQATK
                     EAKAKSEAAAEEPAQQLTIWRETKKPR"
BASE COUNT          528 a         1022 c          893 g          488 t
ORIGIN      
        1 aggattgacg aggcaattga ttctgctata gtgcccaacc atgtggcgct ttcgaatctc
       61 ccgactcgtc ttcacgctgg tgcagaacat cctgtgccgt ctcgtcgtcg ccggccgcga
      121 gaacatcccc gccgaaggcc cctacatcct ggccgtcaat catctgagca cggccgatat
      181 cggcatcatc ttcgtcggct tcccggccca ggaatggcgc tacttcgccg gcgagaagtg
      241 ggccgaccat cccctgtggg ggccgttgat gtcctggctg ggcgtcatct tcatcaaccg
      301 cggcgaggtc gaccggcggg cattacgcga agccctcgtt gcgctggagg aaggggcgat
      361 cttcgccctc gcgcccgaag gtacgcgcag caagacgggc gccctgcagc cgggcaagga
      421 cggatccgcc tttctggcct cgcaagcggg cgtgcccatt ctgcccgtcg gcatcagcaa
      481 cagcgacgtg ctttttgccg atttccgccg cttgcgccgc gccaccatca ccatgcgcat
      541 cgggcggccg ttctcgctgc ccgacctggg ccgtcgcccg cgcggccgcg acctgagcga
      601 tttcacctgc ctcatcatga cccacatcgc cgccctcatc gagccgcgcc atcgcggcgc
      661 ctatggtgac accccggcgc tggccgctct cctggccggc gacgacccgt ggccggcctg
      721 cgccggcgcg agcgtgatga gcgaagagcc ggagcagagc gcgccgtgag ccggccccgt
      781 cgggctatag ataatctgtt ctgaaatttt aaggcaagcc tattgcgaac gcccccgcgc
      841 catgctataa tgcccctctc ttgaccagat aagccgttgt ataggtagcc tacccccata
      901 tatgggggag tggatgctct ggccgtctga caggaatccc aggaagtcca catatattgc
      961 gaggaggtat tgagtatcat gtctagcgcc gtcactcctg ccgttgagtc atcgaccctg
     1021 accctgcccg agaacagtat ggccgtgctt cgccgtcgct atttgcgtcg tggccccgat
     1081 gggcagccca ttgaaaccgt agaggagatg ttccggcgcg tggcccatca tgttgccgcg
     1141 gtagaggccg atcatggcgg cgacaccatc gctgccgaaa acgccttcta taacctgatg
     1201 actgagctgc gcttcttccc caacagcccg accttcaccg gcgcgggcac gccgctgggc
     1261 cagctggcgg cctgcttcgt gctgcccatc gccgacgaca tggggcgcga cccggcgggc
     1321 attttccaga ccctgcgcga cgccgcgctc atccagcaga cgggcggcgg caacggcttc
     1381 gccttctccc gcctgcggcc gaaagacgac cacgtcaaga cgtcggccgg caaggccacc
     1441 gggccggtcg gcttcctgcg cgtctacgac caggccttcg gcgagatcgc ccagggcggc
     1501 acgcggcgcg gggccaacat ggccgtgttg cgcgtggacc atcccgatat attcgagttc
     1561 atctcctgca aggccagcga atacgccatc accaatttca acatctccgt cggcgtcacc
     1621 gacaagttcg tccaggctgt gaaggacgac ggcgatttcg acctgatcaa cccccgcgac
     1681 ggcaaggtat ggcgcaccgt gcgcgcccgc gacatcttcg acgagatcgt caagtacgcc
     1741 caccgcaacg gcgagccggg cgtcctgttc ctcgacgccg ccaaccgctc caaccccgtg
     1801 ccgcacctct acgatctgga agcgaccaac ccctgcggcg agcaatggct cggcccgtat
     1861 gagaactgct gtctcggctc gatcaacctc ggccaacatg tgaccgaaga cgggaagatc
     1921 gactgggagc tgctgcgccg ctccaccgag gaatcgaccc acttcctcga caatgtcgtc
     1981 accgccaacg cctacgtccc ggccgtgccc caactgcgcg tggccgcgct caacgcccgc
     2041 cgcatcggtc tgggcatcat ggggctgggc gacctgttct atcgcaccgg cgtgcgctac
     2101 ggcagcgaag agggccaggc attggccggg cagctcttcg agttcatccg ctaccacagc
     2161 atgcgcacca gcatggaatt ggcccgcgag cgcggcgcgt tcctggccat cgagggcagc
     2221 atctacgacc ccgccgattt caaatggtcg ccgcccaggc cgctggtcaa gggccgcaag
     2281 gattacggcc gccccgccgt cgactggaag gggctggtgg ccgatatcaa ggctcacggc
     2341 attcgcaacg ccgcgcagac gaccgtcgcc cccacgggca ccatcgccac ggtggccggc
     2401 atcgagggct acggctgcga gccggtgttc gccctggcct acatccgcca cgtcaacgac
     2461 aacggcaagg atttgcagtt gcagtacacc agccccctgt tcgagcaggc cctgctcaag
     2521 gccgggctgg atgaggccac gcgcgacgag atcatcgccg aagtcaaccg cgtcggcagc
     2581 tgccagcaca tcgaccggct gccggaagcg attcgtaaca ccttcgtcgt ctcgtccgac
     2641 atcgcgccgg aagagcacgt gcgcatgcag gccgcgattc aggccttcgt cgataacagc
     2701 atctccaaga cgtgcaattt cccggccgga gccacggccg aagatgtcgc cgcggtctat
     2761 atgatggcct gggagctggg ctgcaaggga ttgacggtct acgtcaccgg atcgcgcgag
     2821 acggtgacgc tggagacgca ggccaccaag gaggccaagg ccaaaagcga ggccgcggcc
     2881 gaggaaccgg cgcagcaact caccatctgg cgcgagacga agaagccgcg c
//
LOCUS       DAOMQG010000400         2926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13746_length_2926_cov_8.425636, whole genome shotgun sequence.
ACCESSION   DAOMQG010000400 DAOMQG010000000
VERSION     DAOMQG010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13746_length_2926_cov_8.425636"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1363
                     /locus_tag="PLC06_15125"
     CDS             134..1363
                     /locus_tag="PLC06_15125"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRO91829.1"
                     /translation="MLWKRLSSVLIATGLLLAACGGGSKPGEIPPPPTEALATAAAEV
                     AATISPVPPTPTVAATPLPPAIVTPTTVPAPPTPEVTPTPDTVVLAPEAAAFAGRNPI
                     TGETAAGPESLNRRPIAVKLSNAPADYTRPQAGLNDTDIIFEHWTEGAVTRFTAIFYD
                     TVPPNVGPVRSARLIDLELPAMYDAMLAFSGASVGVNHRLNDSDFSDRLLRADTPGFY
                     RTGDTSKPFEHTLYIHMDQMWQAVADKGLNTAPRFGTYNAFSEMAPEGGSPAGTIAID
                     YKTEKVEWRWDAEIGKFRRWMDFEEHVDANTGEQVTAANVVFLTPYHVNDANICEQIN
                     NGVCAALSIEIQLWGSGPAIVFRDGQSFDVTWHREGRNDQLTFTDAAGNPFPLQIGNT
                     WVQLVPGYLPNPLTITP"
     gene            complement(1470..2102)
                     /locus_tag="PLC06_15130"
     CDS             complement(1470..2102)
                     /locus_tag="PLC06_15130"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRO91830.1"
                     /translation="MEKLDLRKQYKWLYSPSARKVEYVDVPPLLFVRADGTVAAGVGP
                     SDSPDFREAVAALYGISYTLKFMVKKRADNPIDYPVMFLEGLWVTESRTYDTAAREAM
                     DFTVMIMQPDFITADLFEEARAALLRKNPGSGPARVRLETYHEGPAIQTLHIGPYATE
                     PETVVRLEAYAAEHEYAQRGHHEIYLGDPFRAAPEKLRTILRHPVVAMRH"
     gene            complement(2106..>2926)
                     /locus_tag="PLC06_15135"
     CDS             complement(2106..>2926)
                     /locus_tag="PLC06_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRO91831.1"
                     /translation="YDETDLSLYALSIGAAADPMDASELKFVYELSRDGFQALPTYAV
                     TFPFSLLWQITAVPGLRFNPALLLHGEQYLEMKRPLPTRATVTNKARIANIYDKGSGA
                     LVLLDVDTEDEQGQVIACNRVSLFIRGLGGFGGERGPSSSGPTLPDRAPDVIHSEKTN
                     ANQALFYRLTSGDRNPLHADPAFAAAGGFERPILHGLCTFGFAGRAVLRHFAGNDPVR
                     LRSIRSRFTRHVFPGETIITEAWTVDNDNKRVLFQCKVAERDEIVLANGVAEIV"
BASE COUNT          543 a          974 c          891 g          518 t
ORIGIN      
        1 ctcccatagg gaacaagtcg gcaattcgta acgtcttccc tttataattt tcgtacaata
       61 ctcattaacg tccgccgggc gcgatttatc gtgcgtgccg ggcgggtaat tctgcaatcg
      121 agtgtaaaac atcatgcttt ggaaacgact atcatcagtg cttatcgcca ccggtttgct
      181 tctggccgcc tgtggcggag gcagcaagcc gggagagatt cccccgccac cgaccgaggc
      241 gctggccacg gccgcggccg aagtcgccgc gaccatctcg cccgtgccgc ccacgcccac
      301 cgtggccgcc acccccctgc ccccggccat cgtgacgccg acgaccgtgc ccgccccgcc
      361 gacgccggaa gtgacgccga cacccgacac cgtcgtcctg gctcccgaag ccgcggcgtt
      421 tgccgggcgc aacccgatta cgggcgagac ggcggccggc ccggagtcgc tcaatcgccg
      481 tcccatcgcc gtcaagctgt ccaatgcccc ggccgactac acgcggccgc aggcgggcct
      541 caacgacacc gacatcatct tcgagcattg gaccgaaggc gcggtcacgc ggttcacggc
      601 gatcttctac gataccgtgc cgcccaatgt cggcccggta cgcagcgccc ggctcatcga
      661 tctggagctg ccggcgatgt acgacgcgat gctggccttc tccggcgcga gcgtgggcgt
      721 caatcatcgc ctcaacgact ccgacttctc cgaccgcctg ctgcgagccg acacgccggg
      781 cttctatcgc accggggaca ccagtaagcc gtttgagcac acgctctata tccacatgga
      841 tcagatgtgg caggccgttg ccgacaaggg gttgaacacc gcgccccggt tcggcaccta
      901 caacgccttc agcgagatgg cccccgaagg cggttctccg gccggaacga tcgccatcga
      961 ctacaaaaca gagaaggtgg agtggcgatg ggacgcggaa atcggcaagt tccgccgctg
     1021 gatggatttc gaggaacatg tggacgccaa caccggggaa caggtgacgg ccgccaatgt
     1081 ggtcttcctg acgccctacc acgtcaacga cgccaacatc tgcgagcaga tcaacaatgg
     1141 cgtgtgcgcg gcgctgtcga tcgaaatcca gctgtggggt tccggcccgg cgatcgtctt
     1201 ccgcgacggc caaagcttcg atgtgacctg gcaccgcgaa ggccgcaacg atcagctgac
     1261 gttcaccgac gccgccggca atccgttccc gttacagatc ggcaacacct gggtgcagct
     1321 ggtgcccggc tatctgccga acccgctgac gataacgcct tgacgaatga cgaagtgccg
     1381 aatgacgcgc tcctcattcg gcacttcgtg ctccgtaact atttcggcct cggccgccgc
     1441 gccggcccat gagatgtctt gcagggtttt tagtgacgca tggccacaac cggatgtcgc
     1501 aaaatcgtcc tcagcttctc cggcgcggcg cgaaacgggt cgcccaggta gatctcgtga
     1561 tggccgcgct gcgcgtactc gtgttcggcg gcgtaggcct ccagccgaac gaccgtctcc
     1621 ggctcggtgg cataggggcc gatgtggagt gtctgaatcg ccggcccctc gtggtatgtc
     1681 tccagccgca cgcgtgccgg gcccgagccg gggttcttcc gcaataaggc cgcccgcgct
     1741 tcctcaaaca ggtcggccgt gatgaaatca ggctgcatga tcatcaccgt gaaatccatc
     1801 gcctcgcgcg cggccgtgtc gtaggtgcgc gattccgtca cccataaccc ttcgaggaac
     1861 atgaccgggt agtcaatcgg gttgtcggcg cgcttcttga ccatgaactt gagcgtgtag
     1921 ctgatcccgt aaagcgcggc gacggcctct ctgaaatcgg gcgaatcgct cggcccaacg
     1981 ccggccgcaa ccgtaccatc ggctcgcaca aacagcaacg gcggtacgtc gacatactcc
     2041 acctttctcg ccgatgggct ataaagccat ttatactgct tgcgaaggtc cagcttttcc
     2101 atagattaca cgatttccgc caccccgttc gccagaacga tctcgtcccg ttcggcgact
     2161 ttgcattgga acagcacgcg cttgttgtcg ttatcgactg tccaggcctc ggtgataatc
     2221 gtctcgccgg gaaagacatg gcgggtgaag cgcgagcgaa tgctgcgcag ccggaccggg
     2281 tcgttgccgg cgaaatggcg caacaccgcc cgccccgcga agccgaacgt gcacaacccg
     2341 tgcaggatcg gccgctcgaa gccaccggct gcggcaaacg ccgggtcggc gtgcagcggg
     2401 ttgcggtcac ccgacgtcag ccgatagaag agcgcctggt tggcgttggt cttctcggag
     2461 tggataacgt cgggggcgcg gtcgggcagt gtcgggccac tcgacgatgg accgcgctcg
     2521 ccgccgaagc cgcccaggcc acggatgaag agcgacacgc ggttacaggc gatgacctgc
     2581 ccctgttcgt cctccgtgtc cacgtcgagc agcaccagcg cccccgaacc tttgtcgtag
     2641 atgttggcga tgcgggcctt gttggtgacg gtggcgcggg tgggcagcgg ccgtttcatc
     2701 tccagatatt gctcgccgtg gagcagcagc gccggattaa agcgcaagcc cggcacggcg
     2761 gtgatctgcc acagcagcga gaaggggaac gtcacggcgt aggtcggcag cgcctggaaa
     2821 ccgtcgcggc tcaattcata aacgaacttc aactccgatg cgtccatcgg gtcggccgcc
     2881 gccccaatcg acagggcata gaggctcagg tcggtctcgt cataac
//
LOCUS       DAOMQG010000401         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13914_length_2897_cov_10.261436, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000401 DAOMQG010000000
VERSION     DAOMQG010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13914_length_2897_cov_10.261436"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1638
                     /locus_tag="PLC06_15140"
     CDS             121..1638
                     /locus_tag="PLC06_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRO91832.1"
                     /translation="MSPTSQSGNAETAGKGIVLPSRPTILEINVRLWLRELSRLYGHH
                     IDLTNIPTDVWDYFAGFQFDAVWLMGVWERSPAGLAFALQNDALQKAFAAALPHAEPE
                     DIVGSPYCVRQYEADLVIGGREGLAAARAELAARGMGLILDFVPNHVAPDHPWIHSHP
                     EYFIHGTAEDLATTSGEFFEADGEIIAHGRDPYYAPWPDVAQLNGFDPGLRAAMIDLL
                     RDIAGQCDGLRCDMAMLMTTEVFTRTWGERAGPPPADEYWHEIIPAVRESNPHFCFIG
                     EVYWNMEWEMMKQGFDFCYDKRLYDRLIHETAAEVRGHLAAETAYQDKLVRFIENHDE
                     LRAAQVFVGGRALAAAIATYTLPGAKLFHQGQFEGATVRLPVFLTRRPEEPVDRALHA
                     FYRRLAIEIQAPALREGQWRLCTLTGWPDNRSFRNLVAWCWQAGDERRVVVVNLSSST
                     SDARVQLPWPELAGQNWAFVDVMDDTTYEWNGDEVLNMGLYVSLAPWGRHFFAVR"
     gene            1768..>2897
                     /locus_tag="PLC06_15145"
     CDS             1768..>2897
                     /locus_tag="PLC06_15145"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRO91833.1"
                     /translation="MSENLNPFAIAQAQLDKAAGILQLAPDMHAFLREPMREFHFSIP
                     VRMDDGHVQTFKAFRVQYNDARGPAKGGIRFHPDETIDTVRALAAWMTWKTAVCDVPL
                     GGGKGGVVCNPRNMSAGELERLSRGYMRTVARFIGPKQDVPAPDVGSTPQIMAWMLDE
                     YEAIMGHHEPGVITGKPILLGGSAGRTPATAMGGMVTIREATKLLGMEVKGATCAIQG
                     YGNVGSFAHRLAHNLLGLKVVAVSDEFGAIYSDKGLDPAVVSAQMRRTGKVGGTPGSE
                     EIGNTQLLELPVDILIPAAIENQLTGANADRIQAKVIAELANGPTTPEADAIFRQKGI
                     YIIPDFLCNAGGVSVSYFEMVQNTYGYYWTEEVVQERLDQKMT"
BASE COUNT          539 a          903 c          939 g          516 t
ORIGIN      
        1 gggcattacc gtagccaaaa gcgtgggcat gccctccgat aagccgacga gatggccgga
       61 aggttgcaaa atcggcgcgg gtgagtcaga attagtattg ttcgtgaggg agtattcaac
      121 atgtcgccaa ccagccaatc cgggaatgcg gagactgcgg ggaagggcat cgtactgcca
      181 tcgcgcccca ccattctgga gatcaacgtc cggctctggc tccgtgagtt gagccggctc
      241 tacggtcacc acatcgatct gaccaacatc ccaaccgatg tctgggacta ttttgccggg
      301 ttccaattcg acgccgtgtg gctgatgggc gtctgggagc gcagcccggc cgggctggct
      361 ttcgccctgc agaacgatgc cttgcaaaag gcattcgcgg cggcactgcc ccatgccgag
      421 ccggaagata tcgtcggttc gccctactgc gtgcgccaat acgaagccga cctcgtcatt
      481 ggcggccgcg agggattggc cgccgcccgc gccgagttgg ccgcccgcgg catgggcctc
      541 atcctcgatt tcgtccccaa ccacgtggcc cccgaccacc cgtggatcca tagccacccg
      601 gaatacttca tccacggcac ggccgaagac ttggccacca cgtcgggaga gttcttcgag
      661 gccgacggcg agatcatcgc ccacggccgc gacccctatt acgcgccctg gcccgacgtg
      721 gcccagctca acggctttga ccccggattg cgcgcggcta tgatcgacct gctgcgcgac
      781 atcgccggcc aatgtgacgg gctgcgctgc gatatggcca tgctgatgac cacggaagtg
      841 ttcacccgca cgtggggcga gcgcgccggg ccgccgccgg ccgacgagta ttggcacgag
      901 atcatccccg ccgtccgcga gagcaatccc catttctgtt tcatcggcga agtgtactgg
      961 aatatggaat gggagatgat gaagcagggg ttcgacttct gctatgacaa gcggctctac
     1021 gaccggctga ttcatgagac ggcggccgaa gtgcgcggcc acctggcggc cgagacggcg
     1081 tatcaggaca agctggtgcg cttcatcgag aatcatgacg agcttcgcgc ggcccaggta
     1141 tttgtcgggg ggcgcgcgct ggccgcggcc atcgccacct acacgctgcc cggcgccaag
     1201 ctgttccatc aggggcagtt cgaaggggcg acggtgcggc tgccggtctt cctgacgcgg
     1261 cgacccgaag agcctgtcga tcgcgcactc cacgccttct accgacggct ggccatcgag
     1321 attcaggccc cggcgctgcg cgagggacaa tggcgacttt gcaccctgac cggttggccg
     1381 gataatcgca gctttcggaa tctggtggcc tggtgctggc aggctggcga cgagcggcgc
     1441 gtcgtcgttg tgaacctgtc gtccagtaca tcagacgcgc gggtacaact accctggccg
     1501 gagctggccg ggcaaaactg ggccttcgtc gacgtgatgg acgacacgac gtatgaatgg
     1561 aacggcgacg aggtcctgaa tatgggcctt tatgtcagtc tcgccccgtg ggggcggcat
     1621 ttcttcgccg ttcgctgact tggcgcctga tgcgcccatg ctcattacga ataaaaaaca
     1681 gtatcgccag gggccatggg tgcctggcga ttttagttgc tgtaaatggt atatcccata
     1741 tttcctatag gagatctgaa cccaatcatg tccgaaaatt tgaacccgtt tgcgattgcg
     1801 caagcccaat tggataaggc agccgggatt ctgcaattgg cccccgacat gcacgcgttt
     1861 ctgcgcgagc cgatgcggga gttccacttc agcatccccg tccgcatgga tgacggccac
     1921 gtgcagacgt tcaaggcgtt tcgcgtgcaa tataacgacg cccgcggccc ggccaagggc
     1981 ggcattcgct tccatcctga tgagaccatc gacaccgtgc gcgctctggc ggcgtggatg
     2041 acgtggaaga cggccgtgtg cgatgtgccc ctcggcggcg gcaagggcgg cgttgtctgc
     2101 aacccgcgca atatgtcggc cggggagctg gagcggttga gccgcggcta catgcggacc
     2161 gtggctcgct tcatcggccc caagcaggac gtgcccgcgc ccgacgttgg ctcgacgccg
     2221 cagatcatgg cctggatgct ggatgaatat gaggccatca tgggccatca tgagccgggg
     2281 gtcatcaccg gcaagccgat cttgctgggc ggctcggccg gccgcacgcc ggccacggcc
     2341 atgggcggca tggtcaccat ccgcgaggcg acgaagctgc tgggcatgga agtgaaaggc
     2401 gcgacgtgcg ccatccaggg ctacggcaac gtgggcagct tcgcccatcg gctggcccat
     2461 aacctgctgg ggctgaaggt cgtggccgtc agcgacgagt tcggcgcgat ctacagcgac
     2521 aaggggctgg acccggcggt ggtcagcgct cagatgcgcc gcaccggcaa ggttggcggc
     2581 acgcccggca gcgaggagat cggcaatacc caactgctgg agctgccggt ggacatcctc
     2641 atcccggcgg ccatcgagaa ccagctgacc ggggcgaacg ccgatcggat tcaggccaag
     2701 gtcatcgccg agttggccaa cggccccacc acgccggaag ccgacgccat cttccggcag
     2761 aagggtatct atatcatccc cgacttcctc tgcaatgccg ggggcgtgtc ggtgtcctac
     2821 ttcgagatgg tgcagaacac ctatggctac tactggaccg aggaagtcgt gcaggagcgg
     2881 ctcgaccaga agatgac
//
LOCUS       DAOMQG010000402         2890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_13956_length_2890_cov_9.441270, whole genome shotgun sequence.
ACCESSION   DAOMQG010000402 DAOMQG010000000
VERSION     DAOMQG010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13956_length_2890_cov_9.441270"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="PLC06_15150"
     CDS             complement(<1..1462)
                     /locus_tag="PLC06_15150"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRO91834.1"
                     /translation="MQVISSHSQTNRSLGRTLGYFLIPPIVTLALVVLLLATSMALYR
                     ANHSGRVYTGVTIQGIDLGGMTLDEAATALRDAAPYSSSGQLTLVDPRTGQEWPFTPA
                     QLGIVVDTKATAEAAFDVGRSGGPLRRLQDTFQSWYYGRTIAPIVVFDEGQLDRGLGA
                     ISAEIERDAISAAWSTNEAGTPVYSAGQIGRTIDRDYLREELQRPISSFSDARVELLT
                     HDIYPATYDNPEIAARLQRTIASPVTFYFQEPLDDLDLSGVTLPQEELAKWIRVEEVT
                     GADGRTEHLLKVDENAARNWLNQFGTQIYRQPVNARYYFNDDTRELVLVSPHINGREL
                     DVEATLQRFVAQINTDNHAVPLIVKDIVPLAHSGATAVDLGITELITETTTWFSGSSD
                     ERKHNIARAAANFYGIVVAPGEEFSFNRYLGSISETDGYEVGLIIVGGRTIEGVGGGV
                     CQVSTTLYQTAFWAGFPIVERWEHGYMVGYYNDGEGP"
     gene            complement(1636..2331)
                     /locus_tag="PLC06_15155"
     CDS             complement(1636..2331)
                     /locus_tag="PLC06_15155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO91835.1"
                     /translation="MSPPVVILLILPALVALGWLIHWLFIRTEGVFLGRRIVVWLYDL
                     AANRYDGIKQFDADTESAFITWPLRRRLKRFPAPLVLDVATGTGRLPYFLLQEPEFNG
                     RVIGLDASAGMLAQAQVKLAPFGDRAALVRQSALDLPFGDATFAAVTCLEALEFLPDD
                     AAALREMARVLRPGGVLLVSRRQGSDAPWFLGHTHDRDAFEARLADIGLTDIRTQPWQ
                     VEYELVWAARVET"
     gene            complement(2442..>2890)
                     /locus_tag="PLC06_15160"
     CDS             complement(2442..>2890)
                     /locus_tag="PLC06_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HRO91836.1"
                     /translation="FGWKQATVVRRPLGLYAFMYVCLHLTIFVWLDYGFMMTLIVEEI
                     IKRRYAVVGFVAFLLLIPLALTSTTNSQRRLGKRWKSLHQLVYLVGVLAVIHYVWLVK
                     NAYTQPFIFAGIIGFLLLVRVAPIKQWLIATRRRIENRRKAGKGQT"
BASE COUNT          567 a          868 c          962 g          493 t
ORIGIN      
        1 ccggcccctc gccgtcgttg tagtagccga ccatgtagcc atgctcccag cgctcgacga
       61 tggggaaccc ggcccagaat gcggtctgat agagtgtcgt ggaaacctgg cacacgccgc
      121 cgcccacacc ttcgatagtg cggccgccga cgatgatcag gccgacctcg tagccgtcgg
      181 tctcgctgat tgaaccgaga taacgattga aggagaactc ttcgccgggg gccacgacaa
      241 tgccgtagaa gttggccgcc gcgcgggcga tgttgtgttt acgttcgtcg ctggagccgg
      301 agaaccacgt cgtcgtctcg gtgatcaatt cggtgatgcc cagatcgacg gccgtcgcgc
      361 cggagtgggc cagcggcacg atgtccttga caatcagcgg cacggcgtgg ttgtcggtgt
      421 tgatttgggc gacgaaccgc tgcagggtag cctctacatc cagctcacgg ccgttaatgt
      481 ggggtgacac cagcaccagc tcgcgcgtgt cgtcgttgaa atagtagcgg gcgttgaccg
      541 gctggcggta gatctgcgtg ccgaactggt tcagccagtt gcgggcggcg ttttcgtcca
      601 ccttaaggag atgctcggtg cggccgtccg cgcccgtcac ctcttcgacg cgaatccact
      661 tggccagctc ctcttgcggc agcgtgacgc cgctcagatc gaggtcgtcc agcggctcct
      721 ggaagtagaa cgtcaccggc gaggcgatgg tgcgctgcag tcgggcggca atttccgggt
      781 tgtcataggt cgccggatag atgtcgtggg tcaacagttc gacgcgggca tcgctgaagg
      841 agctaatcgg ccgctgcaac tcctcgcgca gataatcgcg gtcgatggtg cggccgatct
      901 ggccggccga gtaaacgggt gtcccggctt cgttggtgct ccaggcggcg ctgatggcgt
      961 cgcgctcgat ctcggccgaa atcgcgccca gcccgcggtc gagttgccct tcgtcgaaga
     1021 cgacgatagg cgcgatcgtt ctcccataat accagctctg gaaggtgtct tgcaggcggc
     1081 gcaacggccc gccgctgcgg ccgacgtcga aggcggcttc cgcggtggcc ttcgtatcga
     1141 caacaatgcc gagttgcgcc ggcgtgaacg gccattcctg gccggtgcgc gggtcaacga
     1201 gggtcagttg gccggaactg gaataaggcg cggcgtcgcg caaagcggtg gctgcttcgt
     1261 caagggtcat gccgccgagg tcgatcccct ggatcgtgac gccggtgtag acccggccgc
     1321 tgtggttggc tcgatagagc gccatgctcg tcgccagcag cagcacaacc agtgccagag
     1381 tgacgatcgg cgggatcagg aaatagccca gcgtgcgccc caggctccga tttgtttgcg
     1441 aatgagatga aataacctgc ataaagcgaa aatatcctgc tataacttgc ggttccctga
     1501 tgagaaccca aggcattcta gcacggccgc cgccggtctg cccgaagact caggtagagc
     1561 ttagggttcc gttcagacct gacaggtggg cgacgcagaa ccttgctccc gcacgaggcc
     1621 tcgcttgccc acctgtcagg tctcgactcg cgcagcccag accagctcat attcgacttg
     1681 ccatggctga gtgcgaatat cggtcaggcc gatatcggcc aggcgcgcct cgaaagcgtc
     1741 gcggtcgtgg gtgtggccca gaaaccaggg tgcgtcggaa ccctgacggc gactgacgag
     1801 cagcacgccc cccggccgca gcacgcgggc catctcgcgc agcgcggccg cgtcgtcggg
     1861 cagaaactcc agcgcttcga ggcaggtgac ggcggcaaag gtggcgtcgc cgaagggtag
     1921 atctagcgcc gactgccgga cgagcgccgc ccggtcgccg aatggggcca gcttgacctg
     1981 ggcctgggcc agcattcccg ccgaggcgtc gaggccgatg acacggccgt tgaactcagg
     2041 ctcctgcagc aggaagtacg gcagccgccc cgtgccggta gccacatcga gcaccagcgg
     2101 cgcggggaag cgcttcaatc ggcgacgcag cggccaggtg atgaaggcgc tctcggtgtc
     2161 ggcgtcgaac tgcttgatgc cgtcgtagcg attggccgcc aggtcataga gccagacgac
     2221 gatccgtcgc cccagaaaga ccccttcggt gcggatgaac agccaatgga tcagccagcc
     2281 aagcgcgacc agcgccggaa ggatgagcag gatgaccaca ggtggagaca tgacagggga
     2341 aaggtaacgc aaaggagaga agatgcaata aagatatgaa agacctgaca ggtgggcgat
     2401 gcggaatctc gttccggccc aaggagttcg tcgcccacct gtcaggtctg gcccttgcct
     2461 gccttgcgcc ggttctcgat ccggcggcgt gtggcgatca accattgctt gatcggcgcg
     2521 acgcggacga gcagcaggaa gccgatgatg ccggcgaaga tgaacggctg cgtataggcg
     2581 ttcttgacca gccagacgta atgaatgacg gccagcaccc cgaccagata gactagctgg
     2641 tggagtgatt tccagcgctt gcccaggcga cgctgggagt tcgtcgtcga ggtgagagcc
     2701 agcgggatga gcagcagaaa ggcgacgaac ccaaccacgg catagcggcg cttgatgatc
     2761 tcctcgacga tgagggtcat catgaagccg tagtcgagcc agacgaagat ggtcagatga
     2821 aggcaaacat acataaaggc atagaggccc agcggccgcc gcacgaccgt cgcctgcttc
     2881 cagccgaaaa
//
LOCUS       DAOMQG010000403         2877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14037_length_2877_cov_5.676116, whole genome shotgun sequence.
ACCESSION   DAOMQG010000403 DAOMQG010000000
VERSION     DAOMQG010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14037_length_2877_cov_5.676116"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PLC06_15165"
     CDS             <1..1202
                     /locus_tag="PLC06_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91837.1"
                     /translation="KRWWAALLISLVLAAAGAWVLWAYPRLGTRPYQEQYLTLMAAHL
                     PLLAWAGVGLFLGVRDATDRFAFLNKSLELFIMAGLFAVAGGIFIGVTAGLFEALGIA
                     LPSIVMRLLIAGGMGLLPVMATAVIYNPRLSPAGQEFDEGLNRLVALLTRTLLPLTAL
                     VLLVYIAFIPFNFRAPFENRDVLITYNAMLFAIVALLTGATPVNLGRVSPRAGRLLRW
                     GIILLAAAAVAIGVYALAAIVYRVTMDRLTPNRLAFIGWNVVNIGLLLAVLYRQARPG
                     SGGWAVGLHRAFAAGAVAYTLWTLVVILALPWLFGINQAQVDSLPPAVQNIIYNQPAP
                     ILLKCNTGPHIYLLEGGQKRWIDTIATFTARGYVWSDVEIISCGDLEAIPDGASIPEG
                     AGRPPGE"
     gene            complement(1241..1720)
                     /locus_tag="PLC06_15170"
     CDS             complement(1241..1720)
                     /locus_tag="PLC06_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HRO91838.1"
                     /translation="MHALDVLKYGNLTVLRALKGLPYDDWNTPGVCGYWSVREIIAHL
                     ASFEYALIDALAVARGAPVGSRLGELLRDGQAFNDAQVAARIGLSPAETLADYEKAHA
                     EAVEAAAALRSSLFYNTGFLPSYGMEYDLEDFITYAFYGHKREHSAQIMVFRDGVGR"
     gene            complement(1864..>2877)
                     /locus_tag="PLC06_15175"
     CDS             complement(1864..>2877)
                     /locus_tag="PLC06_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91839.1"
                     /translation="FLAALVALNRTDALLLVLPALGLVVWRGRSWAALGAAALGFLPF
                     LLWTAFSLAYYGFPFPNTAYAKLNTGIATADLARQGLVYLGNSLRWDPITLTTIGAAL
                     ALAVASRRANWLAIAAGIALYLLYIVRIGGDFMSGRFLTAPLLVAVVVVMLLLDGRLR
                     TRRRQLVAAGVIVALALVGYLPVWLAGNPVGDAARRDGDIADERAYYWPNTGLMNRDR
                     VPHPWATQGLELRAQGPAVIEHGNVGFLGYYAGPDVHIVDRNGLTDPLLARLPVRDPA
                     DWRIGHFRRNVPDGYIDTLRGGRNVIADPAIAALYDDLAIITRGPLWPAARWRAIWRV
                     LRS"
BASE COUNT          469 a          955 c          983 g          470 t
ORIGIN      
        1 gcaagcgctg gtgggcggcg ctgctcatca gcctcgtcct cgccgccgcc ggggcctggg
       61 tgctgtgggc ctatccgcgg ctgggcacgc ggccgtatca ggagcaatat ctgaccctca
      121 tggccgccca tttgccgctg ctggcctggg ccggggtggg gttgttcctg ggcgtccgcg
      181 acgcgaccga ccgcttcgcc tttctcaaca agtcgctgga gctgttcatc atggccgggc
      241 tgttcgccgt cgccgggggc atcttcatcg gcgtcaccgc cgggctgttc gaggcgctgg
      301 gcatcgccct gccgagcatc gtcatgcgcc tgttgatcgc cgggggcatg gggctgctgc
      361 cggtcatggc cacggccgtc atctataacc cgcgcctttc ccccgccggg caggagttcg
      421 acgaagggct gaaccggctg gtggctctgc tgacacggac gctgctgccc ttgacggcgc
      481 tggtgctgct ggtctacatc gccttcatcc cgttcaactt tcgcgcgccg tttgagaacc
      541 gcgatgtgct gatcacgtat aacgccatgc ttttcgccat cgtggccctg ctgacggggg
      601 cgacgccggt caacctgggg cgcgtgtcgc cacgggcggg ccgcctgctg cgctggggga
      661 tcatcctcct ggcggccgcg gcggtggcga tcggcgtcta cgcgctggcg gccatcgtct
      721 accgcgtgac gatggatcgg ctgacgccca accggctggc gttcatcggc tggaacgtgg
      781 tcaacatcgg gctgctgctg gcggtgctct atcgtcaggc gcggccgggg agcggcggct
      841 gggcggtcgg gctgcatcgc gcctttgccg cgggggcggt tgcctatacc ctgtggacgc
      901 tggtcgtgat tctcgccctg ccgtggctgt tcggcatcaa tcaggcccag gtcgatagcc
      961 tgccgccggc ggtacagaac atcatctaca accagcccgc gcccatcctg ctcaagtgca
     1021 acacaggccc ccacatctat ctgctggaag gcggccaaaa gcggtggatc gacaccatcg
     1081 caaccttcac cgctcgcggc tatgtctgga gcgacgtgga gatcatcagc tgtggcgatc
     1141 tggaggcgat ccccgatggg gcgtcgattc cggagggggc ggggcggccg ccgggggaat
     1201 gatgggggct gctccggcgg cgactttttc tttcttcccc ctacctcccc actccgtccc
     1261 gaaacaccat gatctgcgcc gaatgctccc gcttgtggcc atagaaggcg taggtgatga
     1321 aatcttccag gtcgtattcc atgccgtagg agggcaggaa gccggtgttg tagaacagtg
     1381 aggagcgcag ggcggcggcc gcttccaccg cctcggcgtg ggctttctcg taatcggcca
     1441 acgtctcggc cggtgacagg ccaatgcggg cggccacctg ggcgtcgttg aacgcctggc
     1501 cgtcgcgcag caactcgccg aggcgtgacc ccaccggcgc gccacgcgcc acggccagcg
     1561 catcgatcag cgcgtattcg aatgaggcca gatgggcgat gatctcgcgc accgaccagt
     1621 agccgcagac gccgggcgtg ttccagtcgt catagggcag gcctttcagc gcgcgcagga
     1681 cggtgagatt gccgtatttg agaacatcga gagcgtgcat ggatgtaatc ccctatagtc
     1741 aagtggcgat agaataccat gaactgtgcc atggcaacat atttccccgg tcacaattta
     1801 gctccccgct cgtagttgag cgctttagcg cgaccgggcg aaagaacacg gctaaagccg
     1861 ctactacgaa cgaagaaccc gccaaatcgc ccgccatcgc gcggccggcc acaacggccc
     1921 gcgggtgatg atcgccagat catcatagag cgcggcgatg gccgggtcgg cgatgacatt
     1981 gcggccgccg cgcagggtgt cgatgtagcc gtcgggcaca ttgcgccgga aatggccgat
     2041 gcgccagtcg gccgggtcgc gcaccggcag tcgggccagc agcgggtcgg tcagcccgtt
     2101 gcgatccacg atatggacgt ccggcccggc ataatagccc aaaaagccga cgttgccgtg
     2161 ctcgatgacc gccgggcctt gcgcgcgcag ctccagcccc tgcgtggccc agggatgggg
     2221 gacgcggtcc cggttcatca gaccggtgtt gggccagtag taggcccgct cgtcggcgat
     2281 gtcgccgtcg cgacgggcgg cgtcaccgac ggggttgccc gccagccaca ccggcaggta
     2341 ccccaccaac gccagggcca cgatgacccc ggccgcgacc agttggcggc ggcgcgtgcg
     2401 caagcggccg tcgagcagca gcatcaccac gaccacggcc accaacagcg gcgcggtcag
     2461 aaagcggccg ctcatgaagt cgccgccgat acgcacgatg tagagcaggt agagcgcgat
     2521 ccctgccgca atagccagcc agttcgcccg gcgcgaggcg acggccagcg ccagcgccgc
     2581 gccgatggtc gtcagggtga tcggatccca gcgcagcgag ttccccagat agaccagccc
     2641 ctgccgggcc agatcggccg tggcgatgcc ggtgttgagc ttggcgtagg cggtgttggg
     2701 gaacgggaag ccatagtagg ccagcgagaa ggccgtccac agcaggaagg gcaggaagcc
     2761 gagcgcggcc gcgcccagcg ccgcccacga acgcccccgc cacacaacca gccccagcgc
     2821 gggcagcacc agcagcagcg cgtcggtgcg gttgagcgcc acgagggcgg ccagaaa
//
LOCUS       DAOMQG010000404         2875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14050_length_2875_cov_7.626596, whole genome shotgun sequence.
ACCESSION   DAOMQG010000404 DAOMQG010000000
VERSION     DAOMQG010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14050_length_2875_cov_7.626596"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLC06_15180"
     CDS             <1..472
                     /locus_tag="PLC06_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91840.1"
                     /translation="DGAETGVTVHYMGEGLDTGDIATQAAVPLPDGIPQADAERRLML
                     AGVDLVRDVLADLARGVVRRHPQPPGGSYFGFPQPADFALSVEWPARRAYNFMRATAG
                     DDVGYPVEVNGRTEWLATADHYEPNLELDRPSVRHGRHVLIRFNPGILYARLAR"
     gene            477..>2875
                     /locus_tag="PLC06_15185"
     CDS             477..>2875
                     /locus_tag="PLC06_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRO91841.1"
                     /translation="MKTLTRSLWLIISLSVGLIGALLMGRTLAAASAAPAVLIDSVHY
                     DGNALNDADEAVRLVNAGSAMVDLGGWRLSDGSVTVNLPPGTVLNAGAGLWLTGNAAA
                     FRAQFGHDADLTLAPWPGFSNAGDEVILLDSGGTIVDVLVYLAGNTAQTGWSGSALEP
                     YRVAGVFSAEGQILYRWRDPETNVPVPDTNTAADWAQAMDDPVNGRKVRYPGWEGDRF
                     FRPAAITVTSVLTIAVAPDNSYDTLVRLVGNARSSIRLASLMLENAPFAGELAAAARR
                     GVAVTALLEGGPPGGITDQERHACRVVEQAGGACWFMIADAARHIHDRYRYMHAKYMV
                     IDGRISVVGSENFSPDSLPNDDKGDGTWGRRGIFLITDAPAVADYLSTLFNDDLDAGH
                     LDLLRWTAADPVYGAPPPGYTPIMESGGVSYTVRYPAPIVFLDSHAFEIVHAPENALR
                     AGDGLLGLVNRAGEGDLLLVQQLSERPYWGTTTSNPTSDPNPRLEAYIAAARRGAEVH
                     LLLDAFFDAATSLVSNAATCAYVNSTARAEGLSLDCLVANPTGLGIHNKMVLARIDGQ
                     GYVHVGSINGTELSSKGNRELALQVRSNEAFSYLTNLFMGDAPRALYLPLAMRGFRGT
                     VNRLLISEIVYDTPGPDEAEFVELVNATAAPIDLTGYALGDAVLPTDFEDRRFFPPGT
                     IIAAGRPLVVTLSAVAFRAQFGVNPNLEIVDSDPLVPDMIDDPAWGDPKALFQLGNSG
                     DEVVLSRGNAVIDVVTYGDGGYPGVGRCPLLVPPARTLERYPYWDDTDDCGRDFRAWP
                     FA"
BASE COUNT          442 a          971 c          945 g          517 t
ORIGIN      
        1 cgacggcgcg gagaccggcg tcaccgttca ttacatgggt gagggcctgg acaccggcga
       61 catcgccaca caggcggccg tgcccctgcc cgacggcatc cctcaggcgg atgccgagcg
      121 gaggctcatg ctggccggcg tcgatctggt gcgcgatgtg ctggccgatc tcgcgcgggg
      181 ggtggtgcgc cgacatcccc aaccgccggg cggcagctac tttggcttcc cgcaaccggc
      241 cgacttcgcc ctgtcggtcg agtggcccgc tcgccgcgct tataacttca tgcgcgccac
      301 ggctggcgac gatgtgggct atccggtgga ggttaacggc cgcacggaat ggctggccac
      361 cgccgaccat tacgaaccta atctcgaact cgatcgcccc agcgtccgcc acggtcgcca
      421 cgtcctcatc cgcttcaacc ccggcatcct ctacgcccgg cttgcccggt gagcctatga
      481 agacgctgac ccgttccctg tggctcatca ttagcctgtc ggtcgggtta attggcgcgc
      541 tccttatggg gcgaacgttg gctgctgctt cagctgcgcc cgcggtgctc atcgacagcg
      601 ttcattatga cggcaacgct ctcaacgacg ccgacgaggc ggtgcggttg gtcaatgcgg
      661 gttcggcgat ggtcgacctg ggcggctggc ggctgagcga cggcagcgtt accgtcaacc
      721 tgccgccggg gacagtcctc aacgcgggcg caggattatg gctgaccggc aacgcggccg
      781 ctttccgggc gcagttcggc catgacgctg atttgacgct tgccccgtgg cctggcttct
      841 ccaacgccgg tgatgaagtc attctgctgg acagcggcgg gacgattgtc gatgtgctgg
      901 tctatctggc gggtaacacg gcccagaccg gctggagcgg ctccgcgctg gagccgtatc
      961 gcgtggcggg cgtcttcagt gctgaggggc agatcctcta tcggtggcgc gatcctgaga
     1021 ccaacgtccc tgtccccgac acgaacacgg ccgccgattg ggcgcaggcg atggatgatc
     1081 ccgtcaacgg ccgcaaggtg cgctatcccg gctgggaagg cgatcgcttc ttcaggccgg
     1141 cggccatcac cgtcacgtcg gtgctgacga tcgccgtggc ccccgacaat tcctacgaca
     1201 cgctcgtccg gctggtaggc aatgctcgct cgtctatccg tctggcctcg ctgatgctgg
     1261 aaaatgcgcc cttcgcggga gagttggccg cggcggcgcg gcgcggagtg gccgtgacgg
     1321 ctctgctgga gggcgggccg ccgggcggca tcaccgatca ggagcgacac gcctgccggg
     1381 ttgtcgagca ggccggcggg gcgtgctggt tcatgatcgc cgacgcggcc cggcatatcc
     1441 acgaccgcta ccggtacatg cacgccaagt acatggtgat cgacggccgc atctccgtcg
     1501 tcggcagcga gaacttcagt cccgacagcc tgcccaatga cgacaagggc gacggcacgt
     1561 gggggcggcg cggcatcttt ctcatcaccg acgcgcccgc ggtcgccgat tatctgagca
     1621 ccctcttcaa cgacgatctc gacgccggcc acctcgatct gcttcgctgg acggcggccg
     1681 atcccgtcta cggcgcgccg ccgccggggt acacgccgat catggaatcc ggcggcgtca
     1741 gctacaccgt gcgctatccc gcaccgattg tttttctcga cagccacgcc ttcgagatcg
     1801 ttcatgcgcc cgaaaacgcc ctgcgcgcgg gcgacggcct gctcggtctc gtcaatcgtg
     1861 ccggagaggg cgacctgttg cttgtccagc agctctcgga gcggccgtat tgggggacga
     1921 ccaccagtaa cccgacgagt gaccccaacc cccggcttga ggcgtatatc gccgccgcgc
     1981 gacggggggc agaagtgcac ctcttgctcg acgccttttt cgacgctgca accagtctgg
     2041 tcagtaatgc cgctacttgc gcttatgtaa actcaactgc tcgcgcggag ggtctgtctc
     2101 tcgattgcct cgtggccaac ccgaccggcc tgggcatcca caacaagatg gtgctggccc
     2161 ggatcgacgg gcaggggtac gtccacgtcg gcagcatcaa cggcacggag ctttccagca
     2221 agggcaaccg cgaactggcc ctgcaggtgc gctccaacga agccttcagc tatctgacga
     2281 atctgttcat gggcgacgcc ccacgagcgc tctacctgcc gctggcgatg cgcggctttc
     2341 gcggcacggt caaccgcctg ctcatcagcg agatcgtcta tgacaccccc ggccccgacg
     2401 aggccgagtt tgtggaactg gtgaacgcca cggccgcgcc gatcgatctg accggctatg
     2461 ccctcggcga cgcggtgctg cccaccgact tcgaggaccg gcgcttcttc ccgccgggca
     2521 cgatcatcgc tgccgggcgg ccgctggtcg tgacgctctc ggccgtcgcc ttccgggcgc
     2581 agttcggcgt caaccccaat ctggagatcg tcgacagcga tcccctggtg cccgacatga
     2641 tcgacgatcc ggcctggggc gatcccaagg cattgttcca gttgggcaac agcggcgacg
     2701 aggtggtctt gtcgcggggg aacgcggtga tcgacgtcgt cacctatggc gacgggggct
     2761 atccgggggt ggggcggtgt cccctgctcg ttcccccggc gcgaacgctg gagcgttatc
     2821 cgtattggga cgacacggac gattgcggcc gcgactttcg ggcgtggccg tttgc
//
LOCUS       DAOMQG010000405         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14095_length_2866_cov_5.046958, whole genome shotgun sequence.
ACCESSION   DAOMQG010000405 DAOMQG010000000
VERSION     DAOMQG010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_2866_cov_5.046958"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2811)
                     /locus_tag="PLC06_15190"
     CDS             complement(<1..2811)
                     /locus_tag="PLC06_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRO91842.1"
                     /translation="MKPAEFVAKWDRIQQKESAAYVSHFEDLCRLVGHKTPLEYDPGG
                     ENFSFQMATAKTDGRTGFADVFFRGHFILEYKGPHDDLNKAYRQLQLYRESLDNPPLL
                     ITSDIREIHIHTNFTNRPTVTTVVTLGDIAPRPSGAAGPGVEVLRRVFFDPDSFMPEK
                     TRENITRATADTFLAVAESLRQHQRLTGEAYTPEQRAHFLIRLLFCLFAEDLGLLPDG
                     LFTQLVKSQGKTYSDLREPLKKLFATMRGGGHFGMWAIRWFNGTLFDDEFVPTLPADL
                     AGKVRRAAEQDWSAVDPSIFGTLFERIIDEDKRAQLGAHYTGKDDILLIVEPVLMEPL
                     RRKWDEVRRITNYELRITNEEEGRNTKDEVRKEAAGGESAVGGRPSAVVAYDRLVAFS
                     NELAAIRVLDPACGSGNFLYVALRRLLDLQKEVIAFAARRGLPKIPLTVGPQQLYGIE
                     INEYAHELAQVTAWIGYLQWRHENGFDEMEDPVLRPLDNIKRMDAILAHDEAGNPMEP
                     EWPAAEVIVGNPPFLGGFKIRQELGDDRVNDLFKLYEDRVPRTADLVVYWFERAWAMI
                     KSRQSERTGLIATQSIRSNVNRAVLDEIASDGSIFTAWSDKPWVLDGAAVRVSIVGFD
                     DGSEQRKTLDGLPVNHINADLTSSIDLTKAHRLTENLNLSFQGSMIGGPFQIAKTTAM
                     NWISLENPSGRSNSDVIRPYVTGMDVLRRTDEEWVVDFGANTPLEEAQKYFVPFEYVL
                     TMVYPERKDNRRAIRRERWWIHADPQPSMRTAVSQLRRYIATVRVAKHRIFVWLDSSV
                     LCSNKLIVIAREDDYFFGVLHSRVHEIWSMATSSRHGVGNDPAYTPTTTFETFPFPWP
                     PGEEPGIDPDLTGGRSTPGPDETSRAHLSGLEEIARWARALVDWRDAWLNPPPPAKGT
                     LDVAWERLIKARTLTNLYNGLVY"
BASE COUNT          563 a          825 c          866 g          612 t
ORIGIN      
        1 atagaccagc ccgttgtaga ggttggtcag ggtgcgggcc ttgatgagcc gctcccaggc
       61 cacgtcgagg gtgcccttgg ccgggggcgg cgggttcagc caggcgtcgc gccagtccac
      121 cagcgcccgc gcccagcggg cgatctcctc aagacctgac aggtgggcgc gcgaggtttc
      181 gtccggccca ggtgttgagc gcccacctgt caggtctggg tctatgcccg gctcctcccc
      241 cggcggccag gggaagggga aggtctcgaa ggtggtggtg ggggtgtagg cgggatcatt
      301 accaacacca tgtcttgatg aagtcgccat cgaccaaatt tcatgtaccc gcgagtgaag
      361 gactccgaag aagtaatcgt cctctctggc tattacgatc agtttattgg agcaaagcac
      421 cgaagaatcg agccaaacaa atatacgatg ctttgctact cgcaccgttg cgatgtatct
      481 ccttagttgt gatacggccg tgcgcattga aggctgtggg tcagcatgga tccaccatct
      541 ttcccgccga atagcacggc gattatcctt gcgctccggg taaaccattg tcaaaacgta
      601 ttcaaatggc acaaaatatt tttgcgcttc ctctaaaggt gtattcgcac caaaatccac
      661 aacccattct tcatcagttc gccttagaac gtccataccc gtaacgtagg gtcgaataac
      721 atctgaatta gaacgcccac ttggattctc aagtgatatc cagttcatcg cggttgtttt
      781 tgctatctga aatgggcctc cgatcattga accctgaaac gataggttta ggttttctgt
      841 gaggcgatgg gctttggtaa ggtcgattga actggtaagg tcagcattga tgtgattaac
      901 aggtaggcca tccagtgtct tgcgttgctc actgccatcg tcgaatccga caattgaaac
      961 tcttactgcg gccccatcaa gaacccaagg cttgtcagac caagctgtga aaatcgaacc
     1021 gtccgatgca atctcatcga gtacagcccg gttgacattt gagcgaatag attgtgtcgc
     1081 aatcagtccg gtacgctcag attggcggct tttgatcatt gcccaagctc tttcaaacca
     1141 ataaacaaca agatcggcag ttcttggtac cctgtcttca tagagcttaa ataaatcatt
     1201 aaccctatcg tcgcctaatt cttgtctgat cttgaatccc cccaaaaacg gcggattccc
     1261 cacaatcacc tcggccgccg gccattccgg ctccatgggg ttgccggcct cgtcatgggc
     1321 cagaatggcg tccatgcgct tgatattgtc cagcggccgc agcacgggat cctccatctc
     1381 gtcgaagccg ttctcgtgcc gccattgcag atagccgatc caggccgtca cctgggccag
     1441 ctcgtgggcg tattcgttga tctcgatgcc gtagagctgc tgcggcccga cagtcagcgg
     1501 aatcttcggc agcccccgcc gcgcggcaaa ggcgatgacc tccttctgca agtccagcag
     1561 ccgccgcagg gccacataga ggaagttgcc gctgccgcag gcggggtcga gcacgcggat
     1621 ggcggccagc tcattggaga aggcgaccag tcgatcataa gcgacgaccg cggacggccg
     1681 accgccgacc gcggactcgc cgccggcggc ctcttttcgt acttcgtcct tcgtatttcg
     1741 tccttcctct tcattcgtaa ttcgtaattc gtaattcgta attctccgca cctcgtccca
     1801 cttgcggcgc agcggctcca tcagcaccgg ctccacgatg agcaggatgt cgtccttgcc
     1861 ggtgtagtgg gcgcccagct gggcgcgctt gtcctcgtcg atgatgcgct cgaacagcgt
     1921 gccgaagatg gagggatcga cggccgacca gtcctgctcg gccgcgcgcc gcaccttgcc
     1981 cgccaaatcg gccggcaggg tgggcacgaa ctcgtcgtcg aacagggtgc cgttgaacca
     2041 gcggatggcc cacatgccga agtgaccgcc gccgcgcatg gtcgcgaaca gcttcttcag
     2101 cggctcgcgc aggtcgctgt aggtcttgcc ctgcgacttc accagctggg tgaacaggcc
     2161 gtcgggcagc agccccaaat cttcggcgaa caggcagaag agcaggcgga tgaggaaatg
     2221 ggcgcgctgc tccggcgtat aggcctcgcc cgtcagccgc tgatgctggc gcagcgattc
     2281 ggccacggcc agaaaggtgt cggccgtggc gcgggtgatg ttctcgcgcg tcttctccgg
     2341 catgaagctg tcgggatcga agaacacgcg ccgcagcacc tcgacgcccg gtccggcggc
     2401 cccgctgggc ctgggggcga tgtcgccgag cgtgacgacg gtcgtcacgg tggggcgatt
     2461 ggtgaagttg gtgtggatgt ggatctcgcg gatgtcgctg gtgatgagca gcggcgggtt
     2521 atcgagcgat tcgcggtaga gctggagctg gcgataggcc ttgttgaggt cgtcgtgggg
     2581 gcctttgtac tccaggatga aatgaccgcg aaagaagacg tcggcgaaac cggtgcggcc
     2641 gtcggtcttg gcggtggcca tctgaaagga gaagttctcg ccgccggggt catactccag
     2701 cggggtcttg tggcccacga ggcggcacag gtcctcgaag tggctcacat aagcggcgga
     2761 ctctttctgt tgtatccggt cccatttggc gacgaattcg gcgggtttca tgggcgtgat
     2821 tgtagcgcca tccggggaag tgacgagcaa agtacgaggt atgaaa
//
LOCUS       DAOMQG010000406         2863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14105_length_2863_cov_5.131410, whole genome shotgun sequence.
ACCESSION   DAOMQG010000406 DAOMQG010000000
VERSION     DAOMQG010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14105_length_2863_cov_5.131410"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2767)
                     /locus_tag="PLC06_15195"
     CDS             complement(<1..2767)
                     /locus_tag="PLC06_15195"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRO91843.1"
                     /translation="MTTDILSAIRWWAALTILGLAALPLVYSLLRRLPDRGYAFLRPA
                     GLLLVGWVYWLAGTLGFAGNDGGGVVLALLVVAALSWLAWRRRPNTDPPLRDWLRDNR
                     NTVIATELVFAAVFALWVWVRMQNPAISATEKPMEFAFLNGVGRSATFPPLDPWLSGF
                     GISYYYFGYVMVSLLGRLAFVAEPQAFNLAIAWLVAGTATGAFGIVVNLISLRRITND
                     DSCRPTKLRITNEERALHSPLVIRNSSLILGLLAALAIPIAGNMQMGLELLHGNGAGS
                     PALWAWLDVRDIDGPAETTEPARYETSGWWWWRTSRVIHEYHLSGRAEEGLEPIVEIP
                     AFSFILGDMHPHVLALPFAFLSMAVALAWLLEITNYELRITNSEARSSLVSRISYLVS
                     GKVGVPLSLWLFTALVLGGLSFLNTWDVAIHLFLVAGAFFLGHWYRAGTTRGLWGRSA
                     LVGVALAAAAVVLYLPFYLGFRSQAGAPFILPFLMRPTRLVQFLIIFGLPLLAITVWL
                     VVMAARQRFRHWRTGVGIAAMLVLGLLAVMLLWTWLLATSPEFGRVAGVAQELGLTLP
                     PRPDGAIEPAWGAQVVAALLPSLFAARLASPWLALYLGGLLALVVMVLREKVGEENPQ
                     ISQTEEENPQISQISQIEEEGEDDGDARHSPLTTDHRPLTTDHRPLATVFALLLVLTG
                     VLLTLGPEFVYLRDNFGVRLNTTFKFYYQAWAMFGVAAVFGLEYLWATRRRSAAARAT
                     ALITAGAYVALLAVALLFPVYAARSRAAEYRGPARATDGSPLERQPATLDGLAWLARY
                     NPAEHEAIMWLRQQAEAVDGPPPVVLEAVGGQYSGYGRVSANTGLPTVLGWPGHELQW
                     RGSDHAEPGRREPLVGQIYTATDLSLAGALLDRFNVRYIYVGDLEREKYGPVGLEKFG
                     EALDVAFA"
BASE COUNT          560 a          956 c          961 g          386 t
ORIGIN      
        1 tggcgaaggc aacgtcgagc gcctcgccga acttctccag gcccaccggg ccgtatttct
       61 ctcgctccag atcgccgacg tagatatagc gcacgttgaa ccggtcgagc agggcgccgg
      121 caaggctcag gtcggtggcg gtgtagattt gcccgacaag tggctcacgg cggcccggtt
      181 cggcgtggtc gctgccgcgc cattgcagct cgtgccccgg ccagccgagc accgtcggca
      241 gcccggtgtt ggccgagacg cggccgtagc cgctgtactg gccgcccacc gcctcgagca
      301 cgaccggcgg cgggccgtcg accgcttccg cctgctgccg cagccacatg atcgcctcgt
      361 gctcggccgg attatagcga gccagccagg ccaggccgtc gagcgtggcc ggttggcgct
      421 ccagcggcga gccgtcggtg gcgcgggccg ggccgcgata ttcggccgcc cgcgagcgcg
      481 cagcatagac ggggaacagc agcgccacgg ccagcagggc gacgtaggcc ccggccgtga
      541 tcagcgccgt cgcacgggcg gcggccgacc ggcggcgcgt ggcccacagg tattccagcc
      601 caaagacggc cgccacgccg aacatggccc acgcctgata gtagaacttg aaggtcgtgt
      661 tgagccgcac gccgaagttg tcgcgcagat agacgaactc cggccccagc gtcagcagca
      721 cgccggtgag gacgagcagc agggcgaaga cggtggccag tggccggtgg tcagtggtca
      781 gtggccggtg gtcagtggtc agtggggagt ggcgagcgtc cccatcatcc tccccctctt
      841 cttctatctg tgaaatctgt gaaatctgtg gattctcttc ttctgtctgc gaaatctgtg
      901 gattctcttc tcccaccttt tcccgcagca ccatcaccac cagcgccagg aggccaccca
      961 aataaagggc cagccacggc gaggccagcc gggccgcgaa cagcgacggc agcagcgccg
     1021 ccacgacctg cgcgccccac gccggctcga tggctccgtc ggggcgcggc ggcaaggtga
     1081 ggcccagctc ctgggcgacg ccggccacac ggccgaactc cggcgaggtg gccagcagcc
     1141 acgtccacag cagcatgacc gccaacagcc ccagcaccag catcgcggcg atgcccacgc
     1201 ctgtccgcca atgacggaag cgctgccggg cggccatgac caccagccac acggtgatag
     1261 ccaaaagcgg caggccgaag atgatgagga actgcaccag ccgcgtcggc cgcatcagga
     1321 agggcaggat gaacggcgcg cccgcctgcg agcggaagcc gaggtagaac ggcaaataga
     1381 gcacgacggc cgcggccgcc agcgccacgc ccaccagcgc cgaccgcccc cacagcccgc
     1441 gcgtcgtccc cgcccgatac cagtggccca ggaagaacgc cccggccacg aggaacaggt
     1501 gaatcgccac gtcccacgtg ttcaggaacg acagcccgcc gaggacgaga gccgtgaaga
     1561 gccacaggga cagcggaacg cccaccttgc cggatacgag atacgaaata cgagatacga
     1621 gtgaagagcg cgcctctgaa ttcgtaattc gtaattcgta attcgtaatt tccagcagcc
     1681 acgccagcgc cacggccatg ctgagaaagg cgaagggcag ggccagcacg tgggggtgca
     1741 tgtcgccgag gatgaagctg aaggcgggaa tctcgacgat gggttccagc ccttcttcgg
     1801 cgcggccgga gaggtggtac tcgtggatca cccgcgaggt gcgccaccac caccagcccg
     1861 aggtctcgta gcgggccggt tcggtcgttt cggccgggcc gtcgatgtcg cgcacgtcga
     1921 gccaggccca cagcgccgga gagccggcgc cgttgccgtg cagcagttcc agccccatct
     1981 gcatgttccc ggcgatgggg atggcgagcg cggcgagaag accaagaata agtgacgagt
     2041 tacgaatgac gagtggcgag tgaagagcgc gctcttcatt cgtaattcgt aatttcgtcg
     2101 gacgacagga gtcgtcgttc gtaattctcc gcaaggagat caaattcacc acaatcccaa
     2161 acgcccccgt cgccgtgccg gccacgagcc aggcgatggc caggttgaac gcctgcggct
     2221 cggcgacgaa agccagccgc cccagcagcg agaccatgac gtagccgaag tagtagtagc
     2281 tgatgccgaa gccggacagc caggggtcga gcggcgggaa ggtggcgctg cggccgacgc
     2341 cgttgaggaa ggcgaactcc atcggcttct cggtggcgct gatggccggg ttctgcatgc
     2401 gcacccagac ccacagggcg aagacggcgg cgaagaccag ctcggtggcg atgacggtgt
     2461 tgcggttgtc gcgcagccag tcgcgcaggg gcggatcggt gttcggccgc cgccgccagg
     2521 ccagccacga cagcgcggcc acaaccagca gggccagcac aacgccgccg ccgtcgttgc
     2581 ccgcgaagcc cagcgtgccc gccagccagt agacccaacc gacgagcagc agccccgccg
     2641 gccgcaggaa ggcatagccg cgatccggca gccgccgcaa gagtgaatag accagcggca
     2701 gcgccgccag ccccagaatc gtcagcgccg cccaccagcg gatggcggaa aggatgtctg
     2761 tagtcataca gtcggtaggt cagtgggtca gtaggtcagt aagatcagta ggtcagtgag
     2821 tcggtgagat cagtaggccg cccctgaatt cctaattcgt aat
//
LOCUS       DAOMQG010000407         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14176_length_2852_cov_7.101537, whole genome shotgun sequence.
ACCESSION   DAOMQG010000407 DAOMQG010000000
VERSION     DAOMQG010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_2852_cov_7.101537"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLC06_15200"
     CDS             <1..319
                     /locus_tag="PLC06_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91844.1"
                     /translation="VENTDPNLANDIARAWGNRLIQQQNADNDRLRLEDRIYIEFIDD
                     PQVGLERPKTMINTAAGAVFGLLLGVALIFLLEWLASGIIRRSEDVERYLDIPVIGRI
                     PQQ"
     gene            375..1028
                     /locus_tag="PLC06_15205"
     CDS             375..1028
                     /locus_tag="PLC06_15205"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRO91845.1"
                     /translation="MIMNLVTITDPRSPVSEAYRSLRANLSFYSLDKPLRTLMVTSPA
                     AGEGKSTTIANLAVTMAQSGRRTIVVDTDLRRPALHELFGGQMSPGLTDAMMAAAGEL
                     PLQKTAVENLWLLSSGPKPPNPADLLGAERMAQIIAQLTEQADIVLFDAPPVMAAADA
                     AILGARVDGVLLVIQAGKTRRDHSERARETLEKSRAHVVGAALTNAPKDSSMGGYYG"
     gene            1193..2260
                     /locus_tag="PLC06_15210"
     CDS             1193..2260
                     /locus_tag="PLC06_15210"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HRO91846.1"
                     /translation="MKGLILSGGTGSRLYPLTYTNAKQLIPVANKPILFRVIESIRDA
                     GIDDIGIVIGSTGEQIKEAVGRGGRWGVNITYIPQDKPLGLAHAVKTAQPFIGDDRFV
                     MFLGDNVIQGGISSLISQFATSDWNSQIVLTRIEHPEQYGVAEVGDDGRIIRLVEKPK
                     VPPSDLALVGIYMFDHHIFEAADRIKPSWRNELEITDAIQWLVDHGRTVHPYVHQGWW
                     IDTGAPGDMLSANDLVLEEIEHSIEGYVDRDSKVDHRVTVQRGAEIINSTVRGPAIIG
                     ENSRIINSYVGPYTSIDHHVTVENSEIEHSMVLENSKITNIEARIQDSLIGREVIISR
                     SPIRPKALKLTVGDHSKVGIL"
     gene            2421..>2852
                     /gene="dtd"
                     /locus_tag="PLC06_15215"
     CDS             2421..>2852
                     /gene="dtd"
                     /locus_tag="PLC06_15215"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRO91847.1"
                     /translation="MRALIQRVSHAGVTVDGRVTGQIGRGFVVLLGITHGDGRAEADW
                     LAGKIVGLRVFDDDAGKMNRSLADVGGALLVVSQFTLYGDARKGRRPSFTDAAAPEVA
                     EPLVDYFVEVLRGAGFTVATGVFGAHMDVEIHNDGPVTMMIE"
BASE COUNT          566 a          931 c          873 g          482 t
ORIGIN      
        1 ggtggagaac accgacccca atctggccaa cgacatcgcc cgtgcctggg gcaaccggct
       61 catccagcag cagaacgcgg acaacgaccg gctgcggctg gaggaccgca tctacatcga
      121 gttcatcgac gacccgcaag tggggctgga gcggccgaag acgatgatca acaccgccgc
      181 cggcgcggtc tttggcctgc tgctcggcgt ggccctcatc tttctgctgg aatggctggc
      241 gtcgggcatc atccgccgca gcgaggacgt ggagcgctat ctcgacatcc ccgtcatcgg
      301 ccgcatcccg caacaataat cccagtagac ctgacaggtg ggccgccgcg gcccacctgt
      361 caggtctgga atgaatgatt atgaatctag taacgattac cgacccccgt tcgccggtca
      421 gcgaggccta tcgctcattg cgcgcgaacc tgtctttcta tagcctcgac aagccgctgc
      481 ggacgctgat ggtcacctcg cccgccgccg gcgagggcaa atcgaccacc atcgccaatc
      541 tggcggtgac gatggcccag agcggccgcc gcaccatcgt cgtcgatacc gatctgcgcc
      601 gccccgcgct ccacgagctg ttcggcgggc agatgtcgcc cggcctcacc gacgcgatga
      661 tggccgcggc gggcgaattg ccgctgcaaa agacggccgt cgagaatctg tggctgctga
      721 gcagcggccc caagccgccc aacccggccg acctgctcgg cgcggaacgg atggcgcaga
      781 tcatcgccca attgacggag caggccgaca tcgtcctgtt cgacgcgccg ccggtgatgg
      841 ccgcggccga cgccgccata ctgggcgcca gggtcgacgg cgtgctgctg gtcatccagg
      901 ccggcaagac ccgccgcgac cattccgagc gcgcccgcga aaccctggag aagtccaggg
      961 cgcatgtcgt cggcgcggca ttgaccaacg cgccgaagga tagttcgatg ggcggatact
     1021 acgggtagat caattacgaa ttacgaatta cgaattacga ataaagaatt gcttgtcgga
     1081 ttaacgcgct ctcccattcg tcatttcgtc ggccgaccgg ggtcgccttc gtcgttcgta
     1141 gttcgtcgtt cgtcgttcgt aattcgtaat tcgtaattag ttcgggggtt ttatgaaggg
     1201 tctcattctc agcggcggca cggggtcacg actctatccg ctgacctata ccaacgccaa
     1261 gcagctcatc ccggtggcca acaagccgat cctcttccgg gtcatcgagt cgatccgcga
     1321 cgcgggcatc gacgacatcg gcatcgtcat cggcagcacg ggcgagcaga tcaaggaagc
     1381 cgtcggccgc ggcggccgct ggggggtcaa catcacctac atcccccagg acaagccgct
     1441 cggtctggcc cacgccgtca agacggccca gccgttcatc ggcgacgacc gcttcgtcat
     1501 gtttctgggc gacaacgtca tccagggcgg catcagcagc ctcatcagcc agttcgccac
     1561 cagcgactgg aacagccaga tcgtgctgac ccgcatcgag catccggagc agtacggcgt
     1621 ggccgaggtg ggcgacgacg gccgcatcat ccgcctcgtc gagaagccca aagtgccgcc
     1681 gagcgatctg gcgctggtgg gcatctacat gttcgaccac cacatcttcg aagccgccga
     1741 ccgcatcaag ccgtcgtggc gcaacgaact ggagatcacc gacgccatcc agtggctggt
     1801 cgatcacggc cgcaccgtcc acccctacgt gcatcagggc tggtggatcg acaccggcgc
     1861 gccgggggac atgctctcgg ccaacgatct ggtgctggaa gagatcgagc acagcatcga
     1921 gggctatgtc gaccgcgaca gcaaggtcga tcaccgcgtc accgtccagc gcggggcgga
     1981 gatcatcaac agcaccgtgc gcggcccggc catcatcggc gagaacagcc gcatcatcaa
     2041 cagctacgtc ggcccctata ccagcatcga ccaccacgtg acggtcgaaa acagcgagat
     2101 cgagcattcg atggtgctgg agaacagcaa aatcaccaat atcgaggccc gcatccagga
     2161 cagcctcatc ggccgcgagg tcatcatctc ccgctccccc atccgcccca aggcgctgaa
     2221 gctgacggtg ggggatcata gcaaggtggg gattctgtag agagtggatc agtagatcag
     2281 taggtcagtg gatcagtaga tcagtaggtc agtatccact gaataccgac tcactgatct
     2341 actgactcac tgaatactga cccactgaat actgacctac tgaatactga cccactgaat
     2401 actgcctcac tgcaaaaacc atgcgcgccc tcattcaacg agtatcccat gccggcgtca
     2461 ccgtcgacgg ccgcgtgacc ggccaaatcg ggcgggggtt cgtcgtgctg ctgggcatca
     2521 cccacggcga cggccgcgcc gaggccgact ggctggcggg caagatcgtc gggctgcgcg
     2581 tcttcgacga cgacgcgggc aagatgaacc gctcgctggc cgacgtgggc ggggcgctgc
     2641 tggtcgtgtc gcaattcact ctctatggcg acgcgcgcaa ggggcggcgg ccgtcgttca
     2701 ccgacgccgc cgcgcccgaa gtcgccgagc cgctggtcga ttatttcgtc gaagtgctgc
     2761 gcggcgcggg cttcaccgtg gccaccggcg tcttcggggc gcatatggat gtggagattc
     2821 ataatgacgg gccggtgacg atgatgattg ag
//
LOCUS       DAOMQG010000408         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14311_length_2829_cov_7.361211, whole genome shotgun sequence.
ACCESSION   DAOMQG010000408 DAOMQG010000000
VERSION     DAOMQG010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14311_length_2829_cov_7.361211"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1799
                     /locus_tag="PLC06_15220"
     CDS             168..1799
                     /locus_tag="PLC06_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91848.1"
                     /translation="MKRNIWLLIGVLLALMMALVGGAAAEPVAPVGSESEPNDTQATA
                     DAVALGQTSAAISPVGDVDYFKLSLNAGQKVYLAALADCGEWDLETTLAVYDAAGNLL
                     AQAEEWPDDNIIFGFVPPASADYFVRVMSDGYPYSPEELTGNYTLWAVLVPADEPNES
                     VAPVEWGDAFTSTIFAPYDLDYYRIHARPGDVIAATMTVDAVRYYGSSLYINNIDDIY
                     PYFALNTIYAPGTARITFVVPFESDYVIRPVPTFDQCDGLPFPFSYDLTVERLALHVG
                     AAAAGNVNGVSFGTNDIMARDDAGQWRKVFDGEDVGLTTAIQGFEWTSDGALLLALMG
                     NQTLNGPGLVKPQDIVKFTPTQLGDNTQGTFSFYLRGTQAGLTTVGEKIDAIALLGDG
                     GLLISTTGSATVPQLGGGSLGARDEDLLLYHPDGPMPSTGTWTINMDGSRYLSKFGSL
                     DLRVATIAADYPDPDYEPFYHHTWLWFANDKPYKYEGFTANGYKRYTAAPGDLIFMNY
                     YEATYHGPQGDPEWAPLTRQTLGFPKRITNVSIGE"
     gene            1892..>2829
                     /locus_tag="PLC06_15225"
     CDS             1892..>2829
                     /locus_tag="PLC06_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91849.1"
                     /translation="MKRYMWLLIGVLLALLMVLAGGRQATSAQAAGNGMVEKIVFSST
                     RLGSQHDIYVMNLDGSNQTRLTTVSAQDLEPTLSPDGTKIAFASRRNSFQYDIYVMNS
                     DGSGQTRLTTDLGRNEMEPAWSPDGTQIAFVNDNDGIYIMNADGSNPTRLTDFAGDAP
                     SWSPDGTKIAFSYGDIMVIGVDGNNLTNLTNNPSLDTDPEWSPDGTKIVYAGYMADGG
                     GIFVMNADGSGRTRLPNSYGGDYAPAWSPDGTKIVFLSRDDAGHYIAVMDADGGNKTA
                     VSTYNGADHSPDWGAMQGQAWPKTFLPTDDAYVVQS"
BASE COUNT          575 a          849 c          859 g          546 t
ORIGIN      
        1 gatcgcaatg gccgccaagg atgacgctcg cttcccgccg cggttggtca ttgaacatta
       61 ggcgcggccg agccggaaat gacgtccttt cgcggcgggc catgaagggg cagtgggggt
      121 ggcaactcct gtcgctgtcc ccactaacca ctcgtaaaag gaaaatcatg aagcgtaaca
      181 tatggttgtt aatcggagtc ctgctggcgc tgatgatggc gctggtgggc ggggcggcgg
      241 ccgaaccggt tgcgcccgtg ggatctgaat cggaaccgaa tgacacccag gccacggccg
      301 atgccgttgc cctgggccag acatccgcgg ccatctcccc ggtgggggat gtcgattact
      361 ttaaattaag cctgaatgcc gggcagaagg tctatctggc agccctggcc gattgcggcg
      421 agtgggacct ggaaacaacg ctggccgtct atgacgcggc cggcaacctg ctggctcagg
      481 ctgaggagtg gcccgacgac aacatcattt tcggctttgt gccccccgct tcggccgact
      541 acttcgttcg ggtcatgtct gatggctacc cctattcgcc cgaggaactg accggcaatt
      601 acaccctgtg ggccgtcctt gtgcccgccg acgagccgaa cgaatcggtc gcgccggtgg
      661 agtggggcga cgccttcacc tcgaccattt tcgcccccta tgatctggat tactaccgca
      721 tccacgcccg gccgggcgat gtgatcgcgg cgacaatgac cgttgatgcg gttcgttatt
      781 acgggtcgag tctctatatc aacaacatcg acgatatcta cccctatttc gcgctaaaca
      841 ccatctatgc acccggcacg gcccggatta ccttcgtcgt gcccttcgag agcgattacg
      901 tcatccgccc tgttccgacc ttcgatcaat gcgacggtct gccattcccc ttcagctatg
      961 acctgacggt cgagcggctg gcgctgcacg taggcgcggc cgccgccggc aacgtgaatg
     1021 gggtcagctt cggcaccaat gacatcatgg cccgcgacga cgccgggcag tggcgcaagg
     1081 tgttcgatgg cgaagacgta ggcctgacga cggccatcca gggcttcgaa tggacgagcg
     1141 acggcgcgct gttgctggcg ctgatgggca accagacgct gaatgggccg ggacttgtga
     1201 agccacagga catcgtcaag ttcaccccca cccagctggg cgacaacacc cagggcacct
     1261 tcagcttcta tctgcgcggc acccaggccg ggctgaccac cgttggagag aagatcgacg
     1321 ccatcgccct gctgggcgac ggcggcctgc tgatcagcac caccggctcg gcgacggtcc
     1381 cacagctcgg cggcggttcg ctgggcgcgc gggatgagga cctgttgctc taccatcccg
     1441 acggccccat gccctcaacc gggacgtgga cgatcaacat ggatgggtcg cgctatctgt
     1501 cgaaattcgg ctcgctggat ctgcgcgtgg cgacgatcgc cgcggactat cccgaccccg
     1561 actatgagcc gttctaccac catacctggc tgtggtttgc caacgacaag ccctataagt
     1621 atgagggctt tacggctaac ggctacaagc gatatacggc cgcgccaggc gacctgatct
     1681 tcatgaacta ctacgaggca acctatcacg gcccacaagg cgacccggaa tgggcgccgc
     1741 tgacacggca aacgttgggc ttcccgaagc ggatcacgaa cgtgtccatc ggcgagtgac
     1801 gggggaattg cgaattgcga aacaggaatt acgaatccgg tgatcttcct gattcgtaat
     1861 tcctggcttt cgattgatct ggagccaaac catgaagcgt tacatgtggt tgttaatcgg
     1921 agtcctgctg gcgctgctga tggtgctggc gggcgggcga caagcgacaa gcgcgcaggc
     1981 cgctggtaat ggaatggttg agaagatcgt cttcagcagc acccgcctgg gctcgcagca
     2041 tgatatctac gttatgaatt tggacggcag taaccagacc cggttgacga ccgtcagtgc
     2101 gcaggatctc gagcctacct tgtcgccgga cggcacgaag atcgccttcg ccagcaggcg
     2161 caactcattt cagtatgata tttacgtcat gaactccgac ggcagcggcc agacccggct
     2221 gacaaccgat ttgggccgca atgaaatgga acccgcctgg tcaccggatg ggacacagat
     2281 tgccttcgtg aatgataacg acggcatcta tatcatgaac gcggacggaa gtaatcccac
     2341 ccgtctgacg gacttcgctg gtgatgcccc gtcctggtcg ccggacggaa cgaagatcgc
     2401 ctttagctac ggcgatatca tggtcatagg tgtggatggc aataacctga cgaatttgac
     2461 gaacaaccca tcgttggata ctgatcccga gtggtcgccc gacggcacaa agatcgttta
     2521 tgcgggctac atggctgatg ggggcgggat attcgtcatg aatgccgacg gctccggccg
     2581 aacccgcctt cccaatagct acggcggaga ctacgcgccg gcctggtcgc cggacgggac
     2641 gaaaatcgtc ttcctctcca gggatgatgc cggccactac atagcggtga tggatgccga
     2701 cggcggcaac aagacggccg tctctacgta taacggtgcg gatcattccc ccgattgggg
     2761 ggcgatgcag ggccaggcat ggccgaaaac cttcctgccg acggacgatg cctatgtcgt
     2821 tcaatcgaa
//
LOCUS       DAOMQG010000409         2825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14334_length_2825_cov_8.390614, whole genome shotgun sequence.
ACCESSION   DAOMQG010000409 DAOMQG010000000
VERSION     DAOMQG010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14334_length_2825_cov_8.390614"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLC06_15230"
     CDS             complement(<1..794)
                     /locus_tag="PLC06_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRO91850.1"
                     /translation="MPVTTQDAFQFDWHEVARLALISRLMDEMEERELTPQGHVTYQF
                     SARGHELGQILIAQMLNRSMDTASVYYRSRPFMLGSGLTTEEAFASDMARVGSMSEGR
                     DVGVVFNMPRRERALVLPMAADVGSQFTPAAGYAQALCYRRSILRDAAAEGSISVVFG
                     GEGSVAANGFWSALTMATTLNLPLLFVIEDNGYAISVKSRLQTPGANIAANLAAFQGL
                     KIWDGDGCDPATTAGLVAEAVSFVREWRGPALLRLTVPRLSGHSSV"
     gene            complement(928..2172)
                     /locus_tag="PLC06_15235"
     CDS             complement(928..2172)
                     /locus_tag="PLC06_15235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO91851.1"
                     /translation="MLQEIKPATRNRLIAVIFAVSSLVAAAQVAFFTLMPIIAADLGG
                     SESAAGIPSTMGLLFRAAAAYPLGWLMGRMGRRIGLTAGLVVGFIGTAMGAWAIGAGH
                     FWIFSLGAGLAGVARGASDLSRYAAAEVSPIEHRAKILGWIVFAGTIGALAGPLLVTP
                     AVNLAAANGLVPDSGPFWAATIVSGLAAVVTFLFLRPDPLTISRMMDANERINTGRED
                     DEPARPLAQLLKVWHVRLGMAAMSIGQLVMVMIMVITPLHMHHSGHGTGSISFVILAH
                     QLGMYGLSWAAGGLIDRFGATKIIVGGAALLISASLMSPFVTSVAGLSIALFLLGLGW
                     SFCFVAGSALLAVGLAPSERTRVQGMSDTWSSAAAALGSLSTGALFAAGKMLLVGAVG
                     LAFSLGLVALWLIARKRDVVMA"
     gene            complement(2262..>2825)
                     /locus_tag="PLC06_15240"
     CDS             complement(2262..>2825)
                     /locus_tag="PLC06_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRO91852.1"
                     /translation="QTKRAIAIGADSQWKRTHTALLVKSLIYVPLILQNKPIGVLGVT
                     NQIKDSSFDSNDTRALSALGGYATIAINNANMYHSMARERTVLSEIVEHIDDAVIVVD
                     EDFRMLVLNASARALFTLPESNVIGRPIMDLIDHVGLIRMVAEPSIQDDGVRVEHDMI
                     LADGRTYRARVTLVEGGSRFITLRRLG"
BASE COUNT          613 a          906 c          830 g          476 t
ORIGIN      
        1 tcgacgctcg aatggcccga caagcgcggc acggtcagcc gcaacagggc cgggccgcgc
       61 cactcgcgca caaacgaaac ggcctcggca acaaggccgg ctgtcgtcgc cggatcgcaa
      121 ccgtcgccgt cccatatctt tagcccctgg aaagcggcca ggttggcggc aatattggcg
      181 cccggcgtct gcaacctgct cttaacggag atagcgtaac cgttgtcctc gatgacgaac
      241 aggagcggca ggttcagcgt cgtggccatc gtcagggccg accaaaaacc gttggcggca
      301 accgatccct cgccgccaaa aacgacagag atgctgccct cggccgcggc gtcgcgcagg
      361 atcgagcgcc ggtagcacaa agcctgggca tatccggcgg cgggcgtgaa ttgcgaaccg
      421 acatcggccg ccatcggcag caccagcgct cgctcgcggc ggggcatatt gaacacgaca
      481 cccacatccc ggccctcgct catgctgccc acccgcgcca tatccgaggc aaacgcctct
      541 tcggtggtca gcccgctgcc aagcataaac ggccgcgagc gataatagac cgatgcagtg
      601 tccatcgagc ggttgagcat ctgcgcgatg agaatctgac ccagttcatg tcctctggcc
      661 gaaaactgat aggtaacatg gccttgcggg gtcaactcgc gctcttccat ttcgtccatg
      721 aggcgcgaga tgagcgccag ccgggccacc tcgtgccagt cgaattggaa agcgtcttgc
      781 gtggtcacag gcatttctcc ttgtatcgtg atatggaggg ggatcgggtt ccgtaggtgt
      841 tcaacgctat gttatcttcg aatgccggaa ccggccaaac gtcgggtgcg gcatgatcct
      901 ggctcgccgc ggagagttat cgctcgctca ggccatcacc acatcccgtt ttcgcgcgat
      961 gagccacagg gcgaccaaac ccagcgagaa agccaggccg accgcaccaa ccagcagcat
     1021 ctttccagcg gcaaataacg ccccggtgct aaggctgccc agcgcagcgg cggccgacga
     1081 ccacgtatcg ctcatccctt gaacgcgcgt gcgctcgctg ggcgccagcc ccaccgccag
     1141 cagcgcggaa ccggccacga agcaaaaact ccagcccaac cccagcagaa acaaggcgat
     1201 cgacaaaccg gcaacagagg tgacgaacgg gctcatcagc gaagcgctaa tcaacagagc
     1261 cgccccgccg acgataatct tggtcgcgcc gaagcgatcg atcaatccgc cggctgccca
     1321 ggacaggcca tacataccta attgatgggc caggatgacg aacgagatcg agccggtgcc
     1381 gtgacccgaa tgatgcatgt gcaagggagt gatgaccata atcatgacca tcaccagctg
     1441 tccaatggac atggccgcca tgccgaggcg cacgtgccac actttcaaaa gttgagctaa
     1501 tggccgcgcc ggctcgtcat cttcccggcc ggtgttaatc cgttcgttgg cgtccatcat
     1561 ccggctgatc gtcagcggat caggccgcag gaacaggaac gtgacgacag cggccaggcc
     1621 tgacacgatt gtggccgccc aaaacgggcc gctatcaggc acaagcccgt tggcggcggc
     1681 cagattcacg gccggagtga ccaatagcgg gccggccagc gcaccgattg ttccggcgaa
     1741 gacgatccag cccagtattt tggcccgatg ctcaatgggg cttacctcgg ctgccgcgta
     1801 gcgtgacaga tcggacgcgc cacgagccac gccggccaaa ccggccccaa gggaaaagat
     1861 ccagaaatgc cccgcgccaa tcgcccaggc gcccatagcc gtgccgatga agccaaccac
     1921 caggcccgca gtcagcccaa tgcgccggcc catgcgcccc atcagccagc ctaacggata
     1981 ggcggccgca gcgcgaaaaa gcagccccat cgtgctgggg attccggccg cgctctcgct
     2041 gccacccaaa tcggcggcaa taatgggcat gagcgtgaag aaagcgacct gggccgctgc
     2101 taccagactc gagacagcaa aaatgactgc aatcaaccga ttgcgtgtgg caggtttgat
     2161 ttcttgaagc atcggggaat tctgatgcgt tgggcggatt tctgcaaatt gaaggtcgct
     2221 gcagcgacac accttattgc ggttacggtt gttacgttcg cttagcccag gcgacgcaag
     2281 gtgataaacc ggctaccacc ctcgacgagc gtcacccgcg cccgatacgt ccgaccgtcg
     2341 gccagaatca tgtcatgctc cacgcgcacg ccatcgtcct gaatgctcgg ctccgccacc
     2401 attcggatta ggccgacatg gtcgatcagg tccatgatcg gccgaccgat gacgttgctt
     2461 tccggcaggg tgaacaacgc tcgcgccgag gcattgagga ccagcatccg gaaatcttca
     2521 tcgacgacga tcactgcgtc gtcgatatgc tccacgatct cgctcagcac ggtgcgttcg
     2581 cgagccatcg aatggtacat gttggcatta ttgatggcta tggtcgcgta gccgcccagc
     2641 gccgagagcg cgcgcgtatc gttgctgtcg aaggaagaat ccttaatctg gttcgtgacg
     2701 ccgagcacac caatcggctt gttttgcagg atgaggggca catagatcag cgacttgacc
     2761 agaagtgccg tatgggtgcg tttccattgc gagtctgcgc caatagcgat ggcgcgcttg
     2821 gtctg
//
LOCUS       DAOMQG010000410         2821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14362_length_2821_cov_9.224512, whole genome shotgun sequence.
ACCESSION   DAOMQG010000410 DAOMQG010000000
VERSION     DAOMQG010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14362_length_2821_cov_9.224512"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1835
                     /locus_tag="PLC06_15245"
     CDS             174..1835
                     /locus_tag="PLC06_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRO91853.1"
                     /translation="MTDTTTTPSNFIRTIIDEDNAAGKHDGHVHTRFPPEPNGYLHIG
                     HAKSINLNYGIARDYNGQFNLRFDDTNPEKEEQEYIDAIMEDVRWLGADWDGRLFHAS
                     DYFEQLYEWAEQLIRDGKAYVDDLSAEEIRENRGTLTEPGRNSPWRDRTVEENLDLFR
                     RMRAGEYPEGACVLRAKIDMASAFIVLRDPVMYRILHAPHPRTGNAWHIYPTYDWAHG
                     QSDSIEGITHSICTLEFENNRRLYDWFQEALGIPRSQQIEFARLNLTYTVMSKRKMIR
                     LVKEGHVNGWDDPRMWTIRGLRRRGYTSEGIRNFTDMIGVSKANSIVDMSVLEHAVRE
                     DLNLRTPRVMAVLEPLKVVIENYPAGKEETFDIRSWPQDKEREESRSVPFSREIYIER
                     DDFMEEPVKGYKRLAPEEEVRLMNAYVIACTGVVKDKAGRIVELRATYDPATRGGLAP
                     EGRKVKGTIHWVSAEHALPAEVRLYEPLFTQANPEEGVDDFVENLNPDSIRVVDALVE
                     PSLAAAAPGDKFQFVRDGYFCVDLDSEPERLVFNRTVSLKDTWKA"
     gene            complement(1880..2458)
                     /locus_tag="PLC06_15250"
     CDS             complement(1880..2458)
                     /locus_tag="PLC06_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRO91854.1"
                     /translation="MNNFSLQNTRFDLSLPNWAVDALNALPTHLPALEERMAAVIDFS
                     RRNFIEGTGGPFAAGVFERDSGRLVVIGVNCVVPSNCSAAHAEIVALSLAQAMLGTHD
                     LGAAGLPAYQLIASWQPCAMCAAAVPWSGVRSLVVGGHGPDLEAEMGLDKGPTPPGWR
                     VGLEAQGIEVIEGVLYEEARAVFREFASGMCD"
     gene            complement(2466..>2821)
                     /locus_tag="PLC06_15255"
     CDS             complement(2466..>2821)
                     /locus_tag="PLC06_15255"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HRO91855.1"
                     /translation="EIGGWGYNLFKRCGMSTATIEVQPRVDDVTFADAGMLSVRLADG
                     RIVLVPLEWYPRLAHATPTEREDWRVFEDSDKRDIIFWEGLDELIPAIALLIGTPSRE
                     SKRSLDAWLAERKDS"
BASE COUNT          603 a          925 c          807 g          486 t
ORIGIN      
        1 tcgcctcgac gacgcaattg aggtactggc aaaagaataa gagcgtccac agattccaca
       61 gatgacacag ataagaatag tggtcgggcg acaggagccg ccgtaatcag taggtcattc
      121 tcttaaaaat ctgtgaaatc tgtgaaatct gtggattctc ctctggaatc actatgactg
      181 acacgactac aaccccatcc aactttatcc gcaccatcat cgacgaggac aacgccgccg
      241 gcaagcacga cggccacgtc cacacccgct tcccaccgga gccgaacggc tatctccaca
      301 tcggccacgc caagtcgatc aacctcaact acggcattgc ccgcgactac aatgggcagt
      361 tcaatctgcg cttcgacgac accaatccgg agaaagagga gcaggagtac atcgacgcca
      421 tcatggaaga cgtgcgctgg ctgggggccg actgggacgg ccgcctgttc cacgcctccg
      481 actatttcga gcagctctat gaatgggccg agcagctcat ccgcgacggc aaggcctacg
      541 tcgacgacct gagcgccgag gagatccgcg aaaatcgcgg cacgctgacc gagccgggtc
      601 gcaacagccc ctggcgcgac cgcacggtgg aggagaatct tgacctgttt cgccggatgc
      661 gcgcggggga atatcccgag ggcgcctgcg tattgcgggc caaaatcgac atggcctcgg
      721 ccttcatcgt gctgcgcgac ccggtgatgt atcgcatcct ccatgccccg cacccgcgca
      781 ccggcaacgc ctggcatatt taccccacct acgattgggc ccacggccag agtgactcca
      841 tcgagggcat cactcactcc atctgtaccc tggagttcga gaacaaccgt cgcctctacg
      901 actggttcca ggaagcactg ggcatccctc gctcgcagca gatcgagttc gcccgcctca
      961 acctgaccta caccgtcatg agcaagcgca agatgatccg cctggtgaag gaaggccacg
     1021 tcaacggctg ggacgacccg cgcatgtgga cgattcgcgg cctgcgccgt cgcggctaca
     1081 cgtccgaagg catccgcaac ttcaccgaca tgatcggcgt cagcaaggcc aacagcatcg
     1141 tcgatatgtc ggtgctggag cacgccgtcc gcgaagatct gaacctccgc acgccgcgag
     1201 tgatggccgt gctggagccg ctgaaggtgg tcatcgagaa ctatcccgcc ggaaaagaag
     1261 agaccttcga catccgctcc tggccacagg acaaggagcg cgaggaatcc cgctccgtcc
     1321 ccttctcgcg cgagatctac atcgagcgcg acgacttcat ggaagagccg gtgaaggggt
     1381 acaagcggct ggccccggag gaagaggtgc ggctgatgaa cgcctatgtc atcgcctgca
     1441 ccggcgtggt gaaggacaag gccggtcgca tcgtcgagct gcgcgccacc tatgatccgg
     1501 ctacgcgcgg cgggttggcg ccggaaggcc gcaaggtgaa gggcacgatc cattgggtgt
     1561 cggccgagca cgccctcccg gccgaggtgc gcctctatga gccgctcttc acccaggcca
     1621 accccgaaga gggggtcgat gactttgtgg agaacctgaa ccccgactcg attcgcgtag
     1681 tcgatgcatt ggtggagccg agcctggccg cggccgcgcc cggcgacaaa ttccagttcg
     1741 tccgcgacgg ctacttctgt gtcgatctcg attcggagcc ggagcggctg gtgttcaacc
     1801 ggacggtatc gctgaaggat acgtggaaag cctgatcagg ctattataga aaccgggttt
     1861 cttccgagaa acccggtttc taatcacaca ttccactcgc aaactcccga aacaccgccc
     1921 gcgcctcctc atagaggacg ccttcgatca cctcaatccc ctgcgcctcc agcccgacgc
     1981 gccaaccggg cggcgtcggc cccttgtcca gacccatctc cgcctccagg tccggcccgt
     2041 ggccgccgac gaccagcgag cgcacgcccg accacggcac ggccgccgcg cacatggcgc
     2101 acggttgcca gctggctatc agctggtaag cgggcagccc tgccgcgccc agatcgtggg
     2161 tacccagcat cgcctgggcc agcgacaggg ccacgatttc ggcgtgggcg gccgagcagt
     2221 tcgatggcac gacacagttc acgccgatga ctaccagccg gccggaatcg cgctcgaaca
     2281 cccccgcggc gaacggcccg ccagtgcctt cgatgaagtt gcgacgggag aagtcgatga
     2341 cggccgccat gcgctcctcc agcgcgggga ggtgggtcgg cagcgcgttc agcgcgtcca
     2401 ccgcccaatt ggggagagat agatcgaagc gagtattttg cagggagaaa ttattcattt
     2461 gtcgatcaac tatctttcct ttcggcaagc caggcatcaa gcgagcgttt cgattcccga
     2521 ctgggtgtac ctatcagcaa ggcgatcgcg ggaatcagct catccaaccc ctcccagaaa
     2581 ataatgtctc gcttgtcgct gtcctcaaac acgcgccaat cctctcgttc ggtcggggtt
     2641 gcatgggcaa gtcgcggata ccattccagc ggcaccagca cgatacgtcc atctgccaat
     2701 cgcaccgaga gcatgccggc atcggcgaag gtaacatcgt caactcttgg ctgaacctca
     2761 attgttgcag tactcatccc acaccttttg aacagattat acccccatcc accaatttca
     2821 g
//
LOCUS       DAOMQG010000411         2818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14382_length_2818_cov_9.257329, whole genome shotgun sequence.
ACCESSION   DAOMQG010000411 DAOMQG010000000
VERSION     DAOMQG010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14382_length_2818_cov_9.257329"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2818
                     /locus_tag="PLC06_15260"
     CDS             <1..>2818
                     /locus_tag="PLC06_15260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91856.1"
                     /translation="LTPAGTPITLAIKVAVAAGTARRFVVGDPDIQLIDVLAGHTLDQ
                     VAGAEKQTAKGEVVVGEEVARALGDAARITEWRKAYDGNRFAVIESLTQWPEVTPHST
                     LLDLTLPEEEARQWSLLPVYSRLKSGAKFLAELRPAVAMFLKFSGIDYDDDEDAGRKL
                     DAYVRWLQATVQKQEGYLIQVTIGDKGSYAYFSFGAPLAHDDDSIRAVASALDLQHTP
                     PDLDFISDVQIGASRGRVWAGECGANIRHTYGVMGNEVNMAARLMGKATPGQVLVRRR
                     VAEEARAFQFRQLGLITVKGGAEPIPVAELVGRQQIQALQSTQFEQPLVGREDETAQL
                     DRLLDSVLAGKGQLVTVQGPTGIGKSHLLATFQQSAIANGFQVAIGASQHIFQSTTYY
                     PWQQIMRQIFGLKLNPTEQNGDAVDSARIEAIENALAQINPDWSIRVPLLGDLLGLLI
                     PDNPTTAAFDPKQRQQSLFAFVVEVLRTWSRSQPLVIVFENAHWMDDTSDELFEAVAR
                     AIADVPIMIMSVVRSAAETGGTAGAAATVEKLDNYHLLKLAELGQDDIERLIENLLGG
                     PLSLLGTLLISAKAQGNPFFTRELVDTLRESSQLVQVDGKWILSDGMIETMRKANVLV
                     QEEGAWVLAKAADLSTINLGIPDSVHGVILARLDRLPDSHKPTIKIASVIGYSFELGL
                     VAQVHPSSPPGWALRDQAVTLEERDFIIQDWQAHDSPGQDTYTFRQQATQEVSYETLL
                     FTQRRELHCKLAELLERQTPEAVGQIAYHAYLGEDWPRSLRYHLLAGAQDKQLFANLQ
                     SIDHYRKALTSADHLPSRETLVQRQQIHADLGELLLTIGRNDEGQEHLLSALDLAEEL
                     NNPEAQAFVCRWMARAYEVRGQYPPALEWIDRGLAILGDKLTPAALELRLIAGLIFSR
                     RGDYKQAGQQALASMLAAEEINAPS"
BASE COUNT          601 a          777 c          832 g          608 t
ORIGIN      
        1 tcctgacccc ggccggcacg ccgatcactc tggcgattaa ggtcgctgtg gccgcgggca
       61 cggctcgccg attcgtcgtc ggtgatcccg atatccagct gattgacgtg ttggccggcc
      121 atacgctaga tcaggttgct ggcgccgaaa aacaaaccgc taaaggcgaa gttgttgtgg
      181 gcgaagaagt cgcccgtgcg ttgggtgacg cagcccggat aaccgagtgg cgaaaagcgt
      241 atgacggtaa ccggtttgcc gtcatcgaga gcctcacgca atggccggaa gttactcccc
      301 attcaaccct gcttgatttg actttacctg aagaagaagc ccggcaatgg tcactattac
      361 cggtttacag tcgactgaag agcggcgcta aattcctggc tgagttacgg cctgccgtcg
      421 cgatgtttct caagttttcc ggaattgatt atgacgatga cgaggacgcc ggacgcaagc
      481 tggatgccta cgtgcgctgg ctacaggcta ccgtacagaa acaggaaggc tacctcatcc
      541 aggtgacgat cggggacaaa ggcagctatg cctatttctc cttcggcgca cctctggccc
      601 atgatgacga cagcattcgc gcggtagctt cggcgctcga cctgcagcac actccacctg
      661 atctggattt catcagtgat gttcagattg gggcgagtcg cggtcgcgtg tgggccggcg
      721 agtgcggcgc caatattcgc catacgtacg gtgtcatggg taacgaggtg aacatggccg
      781 cccgtctgat gggcaaggcc actccgggcc aggtcctcgt gcgtcgacgg gtggccgaag
      841 aagccagggc gttccagttc cgccagctgg gcttgatcac ggtgaagggc ggagccgaac
      901 cgatcccggt tgccgaactc gtggggcgcc agcaaattca ggcattgcag agcacccagt
      961 tcgaacagcc gttagtaggt cgggaagacg agaccgctca attggatcgt ctgcttgaca
     1021 gcgtattggc cggcaaaggc caattggtaa ccgtgcaggg tccaacgggc atcggcaaga
     1081 gtcacctcct ggcgactttc cagcaatcgg cgattgccaa tggcttccag gtggccattg
     1141 gcgcaagcca gcatattttc caatcgacga cctactaccc ctggcagcag atcatgcgcc
     1201 aaatctttgg tctgaagctg aatccgaccg agcaaaatgg ggatgcggta gatagtgcac
     1261 gcatcgaggc catagagaat gccctggcac agataaatcc cgattggagc attcgagttc
     1321 ccttgttggg cgacctgcta gggctattga tacctgataa tcctacgact gctgcctttg
     1381 atcccaagca gcggcagcaa tccctgtttg cgtttgttgt tgaagtgttg cgcacctggt
     1441 cccgttctca gccacttgtg atagtttttg agaacgcaca ctggatggat gacacatccg
     1501 atgaattatt cgaggcagtt gccagggcaa tcgcagacgt ccccatcatg atcatgtcgg
     1561 ttgtgcgatc ggctgcggaa accggcggta ccgccggcgc ggctgcgaca gttgagaaac
     1621 tggacaacta tcatctgttg aagctggcgg aactgggcca ggatgatatt gagcgattga
     1681 tagagaactt gttaggcggc ccgctatcac tcctgggcac gcttctgatc tcggccaagg
     1741 cacagggtaa tccatttttc acccgtgagt tggttgatac gttgcgcgaa tcaagccagc
     1801 ttgtccaggt tgacggtaaa tggatccttt ccgacggcat gattgagacg atgcgcaagg
     1861 cgaacgtgct ggttcaggaa gagggtgcct gggtgttggc caaggcggcc gatctgtcga
     1921 cgatcaatct ggggattcct gattccgtcc atggcgttat tctggcccgc ctcgatcgtc
     1981 tccctgattc ccacaaacca acgatcaaga ttgccagcgt catcggttac agctttgaat
     2041 tgggtttggt agcccaggtt catccgtcaa gcccgccagg gtgggccttg cgcgatcagg
     2101 ctgttacatt ggaggagcgc gattttatca tccaggattg gcaggcccat gactcgccgg
     2161 gtcaggacac ctacaccttc aggcaacaag ctacacaaga agtatcctat gagacgctgt
     2221 tattcacaca gcggcgcgag cttcattgta aattggccga actgcttgaa cggcaaacgc
     2281 ctgaagctgt gggccagatc gcataccatg cctatttggg tgaggattgg ccccgatccc
     2341 tgcgctatca tttgttggcc ggcgcgcagg ataagcagct attcgccaac ttgcagagca
     2401 tcgaccatta tcgcaaggca ttgaccagcg ccgaccatct gccatcgcgg gagacccttg
     2461 ttcagcgcca acaaattcac gccgacctgg gcgaactttt gctgaccatc ggccgcaatg
     2521 acgaagggca ggaacacctg ctctcggccc tcgatcttgc ggaagaactg aacaaccccg
     2581 aagcacaagc ttttgtttgc cgctggatgg ctcgcgccta cgaagtgcgc ggtcaatatc
     2641 ctccggctct ggaatggatc gatcgtggcc tggcgatcct gggcgataag ctgacgcccg
     2701 cggcgctgga attgcgactc atcgccgggc tgattttttc gcggcgaggt gattataagc
     2761 aggcgggcca gcaagccctg gcgagcatgc tcgcggccga ggagatcaat gcgccgtc
//
LOCUS       DAOMQG010000412         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14423_length_2812_cov_11.451578, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000412 DAOMQG010000000
VERSION     DAOMQG010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14423_length_2812_cov_11.451578"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PLC06_15265"
     CDS             <1..141
                     /locus_tag="PLC06_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRO91857.1"
                     /translation="PPLFLEGLGIPGAHQLKDVTAFTLLVLVLIFRPQGIIGERLAEK
                     KA"
     gene            154..>1265
                     /locus_tag="PLC06_15270"
     CDS             154..>1265
                     /locus_tag="PLC06_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91858.1"
                     /translation="MTKENWRHIIRIGLLMGLIILMTSVIGMVETFNERALVVGYLTL
                     GQLLLYVPALYGGYISVPAGEHRAVPQRLLGGLVAGLLAAVPAILLVLLATSWPDIRT
                     YFVNISPKLITILTSGRAVGPGIGLLAGEMAVLGLIGAAIHLIPDRVRRPIVIALVVT
                     AIFALFSDVPLNIANQLMERNVVRILFGTKGITPLTAVIVFAIALLSAFFWTLKGSSA
                     FQRRRATQTPAQAKRSQQIATIVLLLVLLVLPRVLGIFLTNVLDQVGIFVLMALGLNI
                     VVGFAGLLDLGYVAFFAIGSYVTALLTSTGDLGMGMNFWVAVPIAVVSATLAGIILGI
                     PVLRMRGDYLAIVTLGFGEIIRVLVRSDLLKPVIGGA"
     assembly_gap    1266..1365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1366..1671
                     /locus_tag="PLC06_15275"
     CDS             <1366..1671
                     /locus_tag="PLC06_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine/isoleucine/valine transporter permease
                     subunit"
                     /protein_id="HRO91859.1"
                     /translation="SVFPNSFELLISINVLAIVIVGGIGSLPGVVLGAFVLVGLPELL
                     REFEEYRLLMYGILLITMMLVRPEGLWPSAITRREMHAAEEDAAREGIQAEEVQPLV"
     gene            1682..2497
                     /locus_tag="PLC06_15280"
     CDS             1682..2497
                     /locus_tag="PLC06_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRO91860.1"
                     /translation="MSNILEATNVTKRFGGLVAVNNLTYSIPKGSIVSIIGPNGAGKT
                     TFFNCITGFYKIDEGEFNFDGQSIKGKSPDQITKMGIARTYQNIRLFAYMSAIENILA
                     GMEPRLRSPWFGPVFGLRSTRLEEEKAVVEANQLLNFVGLRGKGDNLARNLPYGDQRR
                     LEIARALAGNPKLLLLDEPTAGMNPRETAETTEFIRNLRDEVGITVLLIEHDMRVVMG
                     ISESITVLDYGEKIAEGTPADIQRNTRVIEAYLGRGAAGAAHLTINQESESGA"
     gene            2494..>2812
                     /locus_tag="PLC06_15285"
     CDS             2494..>2812
                     /locus_tag="PLC06_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO91861.1"
                     /translation="MSILELNNVHAYYGKIHALKGISLTVEKGEIVSLIGGNGAGKTT
                     TLRTICALMRPTTGSVSFNNESLDRYKPSQLTARGIAMVPEGRGVFARMTVLENLEMG
                     AFHR"
BASE COUNT          500 a          833 c          798 g          581 t
ORIGIN      
        1 ccgcccctct tcctggaagg gttgggcatc cccggcgcgc atcagttgaa ggacgtgacc
       61 gccttcaccc tgctcgttct ggtcctcatc ttccggccac agggtatcat cggtgaacgt
      121 ctggccgaaa agaaggccta agggaggcaa tccatgacaa aggaaaactg gcgtcatatt
      181 attcgcatcg gcctcttgat gggtctcatc atcctgatga ccagcgtcat cggcatggtg
      241 gaaacattca acgaacgcgc cctcgtcgtt ggttatctca ctctggggca attgttgctc
      301 tacgtcccgg ccctttatgg cggctacatt tcagttcctg ccggggagca tcgagccgtt
      361 ccccagagat tgctgggagg actggtagcc gggttattgg ccgcagttcc ggctatcttg
      421 ctggtcttgt tggcgacttc gtggcccgac attcgaacct attttgtaaa catctcgccc
      481 aagctgatca ccatactgac ctctgggagg gcggttggcc ctgggatagg gctgctggcc
      541 ggcgagatgg cggtattagg ccttattggc gcggccattc acctgatccc cgaccgcgtc
      601 cgccggccga tcgtcatcgc cctcgtcgtc accgccatct tcgccctttt ctccgacgtg
      661 ccgctcaata ttgccaacca gctgatggag cgtaacgtag tccgcatctt gttcggcaca
      721 aaaggtatca cgccgctcac ggccgtcatt gtgttcgcca tcgccttgct ctccgctttc
      781 ttctggacgt tgaagggcag cagcgctttt caacgccggc gagccactca gacccccgcg
      841 caagccaaac gctcccaaca gatagcgact atcgtcttgc tgctcgtcct gttggtgctg
      901 ccgcgggtgt tgggcatctt tctgaccaat gtgctggatc aggtgggcat ctttgtcctg
      961 atggcgctgg ggcttaatat cgtggttggt ttcgccggat tgctcgacct gggctatgtg
     1021 gcgttttttg ccatcggctc ctacgtgacg gccctgctga cgtccaccgg cgacttgggc
     1081 atgggcatga acttctgggt ggctgtgccc atcgccgtgg tgtccgctac gctggccggc
     1141 atcatccttg gcatcccggt gctgcgcatg cgcggcgact atctggccat cgtcaccctc
     1201 ggctttggcg agatcatccg cgtgctggtg cgttcggacc tgctcaagcc ggtcattggc
     1261 ggcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcggt atttcccaac
     1381 agctttgagc ttctcatttc gattaacgtg ctggccatcg ttatcgttgg cggcatcggc
     1441 agcttgcccg gcgtcgttct gggcgcgttc gtgctcgtcg gcttgccgga actgctgcgc
     1501 gagttcgagg agtaccggct gctgatgtat ggcatcctgc tgatcacgat gatgctcgtg
     1561 cggcccgagg ggttgtggcc ttcggctatc acccggcgcg agatgcacgc cgcggaagaa
     1621 gatgccgcgc gcgaaggcat ccaggccgaa gaggttcaac cgctcgtcta gggagatcgc
     1681 tatgagcaat atactggaag caaccaacgt aaccaagcgc ttcggcggtc tggtcgccgt
     1741 caataatctg acctattcca tcccgaaggg gagcatcgtc agcatcattg gccccaacgg
     1801 cgcgggcaag acgacgtttt tcaactgcat caccggattc tacaagatcg atgagggcga
     1861 gtttaatttc gacggtcaat cgatcaaggg taaatctccc gatcaaatca ccaaaatggg
     1921 catcgcccga acctatcaga acatccgtct cttcgcctat atgtcggcca tcgagaacat
     1981 cctggccggc atggagccgc gattgcgctc accctggttt ggtcccgtct tcgggctgcg
     2041 cagcacgcgc cttgaggaag agaaggcggt cgtggaggcc aatcagctgc tcaacttcgt
     2101 aggcctgcgc ggcaagggcg ataacctggc gcgcaacctg ccctatggcg accagcgccg
     2161 gctggagatc gctcgcgcgc tggccggcaa tccgaagctg ctgctgctcg acgaaccgac
     2221 ggcgggcatg aaccccaggg agacggccga gacgaccgaa ttcattcgca acctgcgcga
     2281 cgaggtcggc atcactgtcc tgctcatcga gcacgacatg cgcgtcgtca tgggcatttc
     2341 ggaaagcatc accgtcctcg actacggcga gaagatcgcc gagggaacgc cggccgatat
     2401 ccagcgcaac acgcgcgtca tcgaggctta tctgggccgc ggcgctgccg gtgcggccca
     2461 tctgaccatc aaccaggaat cggagagtgg cgcatgagca tcctggaact caacaacgtc
     2521 catgcctact acggcaaaat ccacgccctg aagggtattt ccctgaccgt cgagaaagga
     2581 gagatcgtct cgctcatcgg tggcaacggc gcgggcaaga caaccacgct gcgcacgatt
     2641 tgcgcgctca tgcggccgac gaccggctca gttagcttca acaacgagag cctcgatcgg
     2701 tacaaaccga gccaactgac cgcgcgcggg atcgccatgg ttcccgaagg gcgcggcgtc
     2761 ttcgcccgca tgaccgtgct ggaaaacctg gagatgggcg cgttccatcg ca
//
LOCUS       DAOMQG010000413         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14494_length_2799_cov_9.023324, whole genome shotgun sequence.
ACCESSION   DAOMQG010000413 DAOMQG010000000
VERSION     DAOMQG010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_2799_cov_9.023324"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1041
                     /locus_tag="PLC06_15290"
     CDS             217..1041
                     /locus_tag="PLC06_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRO91862.1"
                     /translation="MIISNDPLGCMVRLEDFPSRHVPVRHVDIWLPAGYEADSARRYP
                     VLYMHDGQNCFLPEYCPYDVPWSVQTAMGKLAAAGEIEPAIIVAIWSLAPLRYLEYRP
                     ARPFLYLSELARRRILADLGGWPRSDSYLEFIVEELKPFIDANYRTRTDRAHTAIMGS
                     SMGGLISLYAICEYPDVFGAAGCIATHWPAVEGVIAPYLQGYLPDPATHRIYFDFGTE
                     TLDALYRPTQTLVDALMSDSGYMNGLNWMTRDFPGTRHFEADWAARVHIPLKFMLS"
     gene            1178..2632
                     /locus_tag="PLC06_15295"
     CDS             1178..2632
                     /locus_tag="PLC06_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91863.1"
                     /translation="MNKIRHDQYLSFPIFNLLALSIVLGVLMLAGCLSDIPSAPASIG
                     TLPPAPVATQTAEALSPAYPSPGEPYPDTITDASGNGSSAYPAPATPVGAESGASSAS
                     QEIAATATSEVYFPVIGLEGAATPTVEPAATATVPPPTTTPSPNATQPTVTGPAAIAQ
                     PTPIHVVDFAAARDALAAQGQQLAYNKIGFHVTFLEDRQELDPYMQRLDAEGVPFFLK
                     SVDNAEPLYKAQELMKVSGVPHILVYRSTKYDVPNYDMDPVLAAETYWELEKAIWPPE
                     LDPNIVWLETMNELDKTRSEWLAQYSLRTAELALRDGYKWAAFSWASGEPEPEQWESP
                     AMLELLRLIADNPDRLAIALHEYSYVADDIGHEFPHKIGRFLDLFAVADKHGIPRPTV
                     LITEWGWTYDNIPPEPDAMEDVTWAARLYGAYPQVLGAAIWNLGRLDGELTPLNEQVQ
                     GLIDNMLALSLDSYFSAPAPPQQAPPDASQFPPP"
BASE COUNT          557 a          795 c          823 g          624 t
ORIGIN      
        1 tgtcggggaa tgagccgaac ttgctcatgc ccgtgccgac gatggcgact ccacggttga
       61 gttttttcat agtttgttcc tcgacgcctg ccggggattg gaggcgaatt caacgctttt
      121 tcgatcctgc cggtccattt gaacagggca gatgtcgatg aaagaatatc tttgattata
      181 aaaggtgtgg gtgatgttta ggttacaatc ctcaccatga ttataagcaa tgatcccttg
      241 ggatgcatgg tgcgattgga ggactttcct tcgcgccatg tgccggtgcg ccatgtcgat
      301 atctggctgc ccgccgggta tgaggcagac agcgcgcgcc gttatccggt cttatacatg
      361 catgacgggc aaaactgttt cctgccggag tattgcccct atgatgttcc atggagcgtg
      421 caaacggcga tgggaaagct ggcagcggcg ggggagattg agccagccat catcgtcgcc
      481 atctggagcc ttgctccatt gcgctatctg gaatatcgac cggcgcggcc gtttctctac
      541 ctttccgagt tagcccgtcg acgcattttg gctgacctgg gtggttggcc gcgttcggat
      601 tcctatctgg agtttatcgt tgaggagcta aagcccttca tcgatgccaa ttaccgcacg
      661 cgcaccgacc gcgcgcatac agccatcatg ggttcgagca tgggtgggtt gatctcgctc
      721 tatgccattt gcgagtatcc cgatgtgttt ggcgcggccg gctgcatcgc cacccattgg
      781 ccggccgtcg agggagtgat tgcgccctat ctgcagggat acctgcccga tcccgcgacc
      841 catcgcattt acttcgattt cggcacggag acgcttgatg ctctttatcg gccgacgcag
      901 acgttggtag acgcactcat gtccgattcc ggctatatga atggcctcaa ttggatgaca
      961 cgtgactttc ccggcacgcg acatttcgaa gccgattggg cagcgcgtgt ccatattccc
     1021 ttgaaattta tgttaagttg aaacttcgca aagggttcaa tcgtattata gaggagaagg
     1081 ggcggcagag atgaaccccc catgagggat cgataaattg gccatgggaa ccggtaccat
     1141 gggtctggcg tctatagata tggatgcctt tacctgaatg aacaagatac gtcacgatca
     1201 atatttgagt tttcccatct ttaaccttct cgcgctctct atcgtattgg gtgttctgat
     1261 gctcgccggt tgcctgagcg atatcccgtc cgcaccggcc tcaattggga cgttgccgcc
     1321 tgctccggtg gctacacaaa ccgccgaggc gttgtctccc gcctatccgt cgccgggaga
     1381 accttatccc gacacgatca cggatgcatc gggcaatggc tcctccgcgt atcccgcgcc
     1441 ggcgacgccc gtcggtgctg agtcgggcgc ttcttcggcc agccaggaaa tagccgcgac
     1501 cgctacctcg gaagtctatt ttccggtcat cggcctggaa ggagcagcca caccaactgt
     1561 tgagccggcc gccacagcca cagtgccgcc gcccacgaca acgccatcac ccaacgcgac
     1621 gcaaccaact gtaaccggcc cggcagccat cgcccagccg acgccgatac acgtggtgga
     1681 tttcgcggcc gcccgcgacg cgctggccgc tcaggggcaa caactggcct ataacaagat
     1741 cggttttcac gtgacctttt tggaagacag acaggaactc gatccctata tgcagcgact
     1801 tgatgccgaa ggcgtgccgt ttttcctgaa gagcgttgat aacgccgaac cgctctataa
     1861 agcgcaagag ttgatgaagg tcagcggcgt accccatata ttggtttatc gttcgacgaa
     1921 gtacgacgtg cccaattacg acatggatcc ggttttggcg gcggagacgt attgggagct
     1981 ggaaaaagcc atctggccgc cggagcttga ccccaatata gtatggcttg agacgatgaa
     2041 cgaactcgac aagacccgct cggagtggct ggcgcagtat tcgctgcgca cggccgagct
     2101 tgctttgcgc gacggctata aatgggcggc gttcagctgg gcctcgggcg agccggagcc
     2161 ggagcagtgg gagtcgccgg ccatgctgga actcctgcgc ctcatcgccg acaacccgga
     2221 cagattagcc attgccttgc acgaatacag ttatgtggct gacgacatcg gccatgaatt
     2281 tccgcacaag attggccgtt ttctcgatct gttcgcggta gccgataagc atggcatccc
     2341 gcggccgacg gtgcttatca ccgagtgggg ttggacctat gacaatatcc cccccgaacc
     2401 ggacgcgatg gaagatgtga cctgggcggc gcgactctac ggcgcttatc cccaggtgtt
     2461 gggcgcggcg atctggaacc tggggcggct tgacggggag ttgacgccgc tcaatgagca
     2521 ggtgcagggg ctgatcgaca acatgctggc ccttagtctg gatagctact ttagcgctcc
     2581 cgcgccgccg caacaggcac cgccggatgc gagccagttc ccaccgccgt agatgaaccg
     2641 gggcgagaca accggagaat aacgtgccgg tgattggacg aggctctcgc gccggttgtc
     2701 ccgcccttac agctcgtagg cggttcggat gtcgttcatg atcgcgatgg ccttgcggac
     2761 gaggatttga ttttccggcc ggttgtacca gaggctgac
//
LOCUS       DAOMQG010000414         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14529_length_2795_cov_12.491241, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000414 DAOMQG010000000
VERSION     DAOMQG010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14529_length_2795_cov_12.491241"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLC06_15300"
     CDS             <1..348
                     /locus_tag="PLC06_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91864.1"
                     /translation="GAVVVDLDGDGYAGTGWAITYMHLETRDRLPFGTTVTTGDRLGH
                     PSCEGGFSNGTHLHITRSYNGRWISADGSLPFGMSGWESSGTGTEYDGYLTRGADVRE
                     ACVCREEINAVSR"
     gene            complement(329..754)
                     /locus_tag="PLC06_15305"
     CDS             complement(329..754)
                     /locus_tag="PLC06_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRO91865.1"
                     /translation="MAIKRGDNLFETAGTAEGVWGLSTIFSRIISGELPAAIVYQDDL
                     VTAFRDIDPVAPVHILIVPNKEIATVNDLTDEDERTAGRMLLAARKIAEQEGIAESGY
                     RLIINCNHDGGQEVYHLHMHLVGGRRMGPMVMKPSEKRR"
     gene            832..>2795
                     /locus_tag="PLC06_15310"
     CDS             832..>2795
                     /locus_tag="PLC06_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRO91866.1"
                     /translation="MPTTTLPLPRSKTPAIDIYIRLAHYPVLADKIRARMRHELFSRG
                     IIDEIVFEAQVREKAIESQRRERLTDPYGQEDTHIWQKRKARVRDFQTDALFADNLGS
                     VLLDQIIQEVLLNQPSPSVSSGLNFNPEISPWEVLFRQGEIYEQLPKDEFERSQHHLE
                     EIKVVLIKRLISDQLPYIGVAKRVFTINDLRYIYNRRIGNGKIGGKAAGLMLAWRILQ
                     QQHGDGNDIHPYIGIPESYFIGTEVIYEFRLMNGLDHYMNQKYREIEEIREEYPRIVE
                     AHLAGDFSPGVIKQLRAVLADIGDSPLIVRSSSLLEDNFGYAFAGKYNSYFCPNQGTE
                     EENLIDLLNAVRRVFASTLNPDAILYRQKHGLIDYDERMAILIQKAVGKPYRDFYFPV
                     LAGVAFSQNPFRWNNQIRREDGFLRLVWGFGTRAVDRVSNDYPRLIALSHPQLRPETT
                     TKAIRQYSQWYIDVIDVKKNSFETVHVLDILDGRYPYLRYIASVSRDDYLQEILSTAA
                     LQRDDELILTFNGVTKDRRFVNLMRTALQRLESAYGRPVDVEFVVDILPGYPQTEYRL
                     HVLQCRPLSQRAEDRAVTIPRNIPPEKVLFTSNRLVPDGRAEGVRYIVFIDPQSYYDL
                     HDTTTKHELARAIGRLNKRLENESFILMGPG"
BASE COUNT          631 a          860 c          735 g          569 t
ORIGIN      
        1 ggcgccgtcg tcgtcgatct ggacggcgac ggttacgcag ggacaggctg ggctatcacc
       61 tacatgcacc tggaaacgcg cgatcgattg cccttcggca caactgtaac caccggagac
      121 cgtctgggcc atccgtcctg cgagggcgga ttctccaacg ggacgcacct gcacatcacc
      181 cgcagctaca acggccgctg gatcagcgcc gacggttctc tgccgtttgg catgagcggc
      241 tgggaatcct caggcacagg cacagaatat gacggttacc tgacccgtgg cgccgacgtc
      301 cgcgaggcct gtgtctgccg cgaggagatc aacgccgttt ctcgctaggc ttcatgacca
      361 taggccccat tcgacggccg cccaccagat gcatatgcag atgataaact tcctgcccgc
      421 cgtcatggtt gcagttgatg atgagccgat agccgctttc agcgatccct tcctgctcgg
      481 cgattttccg tgctgccaat aacatccgcc ccgctgtgcg ctcgtcctca tcggtcagat
      541 cattgacggt ggcgatctcc ttgttgggga cgatgaggat atgaacagga gccaccgggt
      601 caatatcgcg gaaagccgtc accagatcgt cctgatagac aatcgctgcc ggaagctcac
      661 cgctgatgat tctcgagaaa atcgttgaca aaccccacac tccttcggcc gtgccggccg
      721 tctcaaataa attatcgcct cgttttatcg ccattcgtgt cggtggccat gtttcacagc
      781 ctgttataat aggggcaggt tattcaaatc cacagttgag ggccggataa aatgcctacg
      841 acgacccttc cgcttccgcg gagcaaaacg ccggcgatcg atatctatat tcgcctggcc
      901 cactacccgg tgttggccga caaaattcgg gcgcgcatgc gccacgagct attcagccgc
      961 ggcatcatcg atgaaatcgt cttcgaagcc caggttcgcg aaaaagcgat cgaatcccaa
     1021 cgccgcgagc gattgaccga tccctatgga caggaggata cccacatctg gcagaaacgg
     1081 aaagcgcggg tgcgtgactt ccagaccgac gcactcttcg ccgacaatct gggtagtgtt
     1141 ttactcgatc agattattca ggaagtcctg ctcaatcaac cgtccccgtc cgtctcctcc
     1201 ggcctcaact tcaacccgga gatatctccc tgggaagtac ttttccgtca aggggaaatt
     1261 tacgagcagc tacccaaaga tgagttcgaa cgcagccagc atcatctgga ggagatcaag
     1321 gtcgtgctga tcaagcggtt aatcagcgac cagctgccct atatcggcgt ggccaagcgc
     1381 gtcttcacca ttaatgatct gcgctatatc tacaatcgcc gcatcgggaa cggcaagatc
     1441 ggcggcaagg cggcggggct gatgctcgcc tggcgcatcc tgcaacagca gcacggggac
     1501 ggtaacgata tccaccccta tatcggtatc cctgagtctt acttcatcgg aaccgaggtc
     1561 atctacgagt ttcgcctgat gaacggtctg gatcattaca tgaaccagaa gtatcgggag
     1621 atcgaggaaa tccgcgagga atacccacgg atcgtcgagg cgcatctggc cggagatttt
     1681 tcgccaggcg tcatcaaaca actgcgcgcg gttctggccg acataggcga cagcccactc
     1741 atcgtccggt cgtccagtct gctggaagat aatttcggct acgccttcgc cggaaaatac
     1801 aacagctatt tctgcccaaa tcagggcacc gaagaggaaa acctcatcga cctgctcaac
     1861 gccgtgcggc gcgtcttcgc cagcactctc aaccccgacg ccatactcta ccgacaaaaa
     1921 cacggcctca tcgattacga cgagcgtatg gccatcctga tccaaaaggc ggtaggcaag
     1981 ccatatcgcg acttctactt ccccgtcctg gccggcgtgg cgttcagcca aaacccattc
     2041 cgctggaaca accagatcag gcgcgaggac gggttcttgc ggctcgtctg gggcttcggc
     2101 acgcgcgccg tcgatcgcgt cagcaacgat tatccacgac tgatcgccct cagccatccc
     2161 caattgcggc cggaaacgac cacgaaagct atccgccaat attcacaatg gtatatcgac
     2221 gtcatcgacg tgaagaaaaa cagcttcgag actgtccatg tcctcgacat tctggatgga
     2281 agatatccct atctgcgcta tatcgcctcg gtcagccggg atgattattt gcaggagata
     2341 ctctccaccg ccgcattgca gcgcgatgat gaactgattt tgaccttcaa cggggtgacc
     2401 aaagatcggc gattcgtcaa tctgatgcgc accgcgctgc agcgtcttga atcggcctat
     2461 gggcggccgg tcgacgtgga gtttgtagta gatatcctgc ccggctaccc gcaaaccgaa
     2521 taccgcctgc acgttctcca atgccgtccg ttgagccagc gtgccgagga cagagccgtc
     2581 accatcccgc gcaacatccc gccggaaaag gttctgttca cctcgaacag gctggtcccc
     2641 gacggccgcg ccgaaggggt tcgctatatc gttttcatcg atccgcaatc ctattacgac
     2701 ctccatgaca caaccaccaa acatgaattg gcgcgggcca tcgggcggct caacaagcga
     2761 ctggaaaatg aatcgttcat cctcatgggg ccggg
//
LOCUS       DAOMQG010000415         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14531_length_2795_cov_6.912774, whole genome shotgun sequence.
ACCESSION   DAOMQG010000415 DAOMQG010000000
VERSION     DAOMQG010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14531_length_2795_cov_6.912774"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1935)
                     /locus_tag="PLC06_15315"
     CDS             complement(64..1935)
                     /locus_tag="PLC06_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HRO91867.1"
                     /translation="MNISRNGHSDNTRLTNDERRAAQADSAVYDYVIIGSGFGGSVSA
                     WRLTEKGYSVLVIERGKRWNADDFPKTNWNVFKYLWMPALRCFGPQGLNFFKDIWLLN
                     GTGVGGGSLIYASTHRIPPKAFFEAPEWAHLADWEAELMPHYHTANRMLGTTTNPLLW
                     PADHALLDIAHDLGQADTFEATGVGIYFGEPGVTVKDPFFEGQGPTRTGCTHCGGCMV
                     GCRHNAKNTLDKNYLYLAEKHGARVLPEHNVNLIRPLFGAQPDEARYEVACEKITDFG
                     RKRRSTVRARNVIVAAGVLGTVELLFNCRDVHGTLPLISQCLGEGVRSNSEALMGVTA
                     RHNRQDFSQGVAITSHFWADDETSVEPVRYPPGSSFMRSLIWPLAAYGDAGHSLGARL
                     VASLRHFLRKPGDFMHTRFLPRWAERDTVLLIMQTVENKMSFRRGRYLSKGFRMGLQT
                     ERDPEQPIPACVTAGSDVVNRFAEKVDGVPWVGLNDLLNIPNTAHILGGCPLGGDATK
                     GVVDIDQQVFNYPGLYVADGSVVPANLGVNPSLTIAAMTERAMSRIPARAKAAPAEPL
                     PYPHLISIDGRPVATINGGGRVVAAPQSGKRPTLAPLLLLLAGLLPLLLLLFGLKKK"
     gene            2305..>2795
                     /locus_tag="PLC06_15320"
     CDS             2305..>2795
                     /locus_tag="PLC06_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91868.1"
                     /translation="MFNRDSLSVEEWAQVIAAPASVGALVVTADPSGPMGLIGEFRAI
                     MKSIKEYVDAHAGDSPLMGALQSHLTTKPSEEEEAQMKEWAQTEKESMKAAKPNTVEE
                     MKQMVRDRTTETFTMLRAKGADEMDINTFKVMLITVAEATAEASKEGGFLGFGGVRVS
                     DAE"
BASE COUNT          525 a          857 c          910 g          503 t
ORIGIN      
        1 tatcattttg gctttgcgct ttttcatgca acaaagccat tcgtaattcg taattttcct
       61 gaattatttc ttcttcaggc caaacagcaa caaaagcagc ggcagcaggc cggcaagcag
      121 caacagcagg ggcgccaatg tgggccgctt ccccgattgg ggcgcggcga caacgcggcc
      181 gccgccgtta attgtagcca ccgggcggcc gtcgatcgag atcaggtgcg gatagggcag
      241 cggttcggcc ggggcggcct tggcgcgggc cgggatgcgg ctcatggccc gctcggtcat
      301 ggcggcgatg gtcaggctgg ggttgacccc caggttggcc gggacgaccg agccgtcggc
      361 cacatagagg ccgggatagt tgaacacctg ctggtcgata tcgaccaccc ccttcgtggc
      421 gtcgccgccc agcgggcagc cgcccagaat gtgggccgtg ttggggatgt tgagcaggtc
      481 gttcagcccc acccagggca cgccgtcgac cttctcggcg aagcggttga cgacatcgct
      541 gcccgcggtc acgcaggccg ggatgggttg ctccgggtcg cgctcggtct gcaggcccat
      601 gcggaacccc ttcgagagat aacggccgcg gcggaacgac atcttgttct ccaccgtctg
      661 catgataagc agcaccgtgt cccgctcggc ccagcgcggc agaaagcggg tgtgcatgaa
      721 gtcgcccggc ttgcgcagga agtgccgcag cgacgccacc agccgcgccc ccaggctgtg
      781 cccggcgtcg ccataggcgg ccagcggcca gatgagcgag cgcatgaacg acgacccggg
      841 cggatagcgc actggctcga cgctggtctc gtcgtcggcc cagaagtggc tggtgatggc
      901 cacgccctgg gagaaatcct gcctgttgtg gcgcgccgtc acacccatca gggcctcgct
      961 gttgctgcga accccctcgc cgaggcattg ggagatgagc ggcagcgtgc cgtggacgtc
     1021 gcggcagttg aacagcagct ccaccgtgcc caacacgccg gcggcgacga tgacattgcg
     1081 cgcccggacc gtggagcggc gcttgcggcc gaaatcggtg atcttctcgc aggcgacctc
     1141 gtagcgggcc tcgtccggct gcgcgccgaa gagcggccga atgaggttga cgttatgctc
     1201 cggcagcacc cgcgcgccgt gcttctcggc cagatagaga tagtttttgt ccagcgtgtt
     1261 cttggcgttg tggcggcagc cgaccatgca gccgccgcaa tgggtgcaac cggtgcgcgt
     1321 cggcccctgg ccctcgaaga acgggtcctt gacggtgacg cccggctcgc cgaagtagat
     1381 ccccacgccg gtggcctcga aggtgtcggc ctggcccagg tcgtgggcga tgtccagcag
     1441 ggcatgatcg gccggccaga ggagagggtt ggtggtcgtg cccagcatgc ggttggcggt
     1501 gtggtagtgg ggcatcagct ccgcttccca atcggccagg tgcgcccatt ccggcgcttc
     1561 gaagaacgcc ttgggcggga tgcggtgggt gctggcgtag atgagcgagc cgccgcccac
     1621 gcccgtgccg ttgagcagcc agatgtcctt gaagaagttc agcccctgcg gcccaaagca
     1681 gcgcagcgcg ggcatccaca gatatttgaa gacgttccag ttggtcttgg ggaaatcgtc
     1741 ggcgttccag cgcttgccgc gctcgatgac cagcacggag tagccctttt ccgtcagccg
     1801 ccaggcggac acagacccgc cgaagccgga gccgatgatg acatagtcgt agacggccga
     1861 atcggcctgc gccgctcgcc gttcgtcgtt ggtcaaccgt gtattgtcgc tgtgcccatt
     1921 acgcgagatg ttcatgttcc cgtcccctga catagcaccg agcgctgcaa tgcctgacgt
     1981 tccaacgtct accatatacg acgcctgatg ctccaacgtc tgacttttcg atttacggga
     2041 tcgcatcgcg atccctcatc aggcattata acacattgcg tcatgatgac gcaatgtgtc
     2101 aacaagcgac cttcagggct tgggggacgc agcattgaat gtcgtcgctc gcggcaatag
     2161 ctgtcgcgaa tcgctccgtg gcgagcacca aaaacgggtt gaacacagag gttgcctgcc
     2221 ggccgatgat ggagtaaaac cggcgcgatt tgtcgtgtca ccatcgacaa tttcaactca
     2281 tttttatcat gcaaggagat atccatgttc aatcgtgatt ccctgtccgt tgaggaatgg
     2341 gcgcaggtca ttgctgcgcc ggcgtctgtg ggggcgctgg tcgtcacggc cgatccgtcc
     2401 ggcccaatgg gcctcatcgg cgagttccgc gccatcatga aaagcatcaa ggagtatgtg
     2461 gatgcccacg ccggcgacag cccgctcatg ggcgctctcc agtcccatct gaccaccaag
     2521 cccagcgagg aagaagaagc ccaaatgaaa gagtgggcgc aaaccgagaa ggaatcgatg
     2581 aaggccgcca agccgaacac ggtcgaagag atgaaacaaa tggtacgcga cagaacaacg
     2641 gagacgttca ccatgttgcg cgccaaaggg gccgacgaga tggatatcaa caccttcaag
     2701 gtcatgctca tcaccgttgc cgaggctacg gccgaagcga gcaaggaagg cggctttctg
     2761 ggctttggcg gcgtgcgcgt ctccgacgcc gagca
//
LOCUS       DAOMQG010000416         2791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14564_length_2791_cov_8.133406, whole genome shotgun sequence.
ACCESSION   DAOMQG010000416 DAOMQG010000000
VERSION     DAOMQG010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14564_length_2791_cov_8.133406"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PLC06_15325"
     CDS             <1..1148
                     /locus_tag="PLC06_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91869.1"
                     /translation="GDVMSGLTADGRIAVPVSGGLDSRSTVATLGRQPGKISPTDEGL
                     WAYSYGYGDDSIETRIAGELATRRGLRFDAFTIRPYLFDRITPVIGAVEGFQDMTQAR
                     QAFIGRELRANADAVIAAHWGDVYLGDMGLAATPPGALSGEAIREHALHKMRKPGGWL
                     IDTVCRPHLGKVEPEELLHSFVAEGMDGLEDIADADFRVKAFKTEQWSARWTTASLRM
                     YQAAVWPRLPFYDTRLGDFFATVPTEFVAGRRLQIDYLKRFAPDLAAVPWQESGSSLF
                     KVGQPDPMAVVRRGFNKGRRILTGRRVIESNWEVQFLNESGRHGLEQWLLRRGLRLHE
                     FVAPPRIEALLTAFQSTPRVDKRSYTVSMLLTFSAWLESFGGGADAP"
     gene            1145..2362
                     /locus_tag="PLC06_15330"
     CDS             1145..2362
                     /locus_tag="PLC06_15330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRO91870.1"
                     /translation="MNGRVDAPLRVLVPGLTWPPETFLARLFRGLAARGIQLTLIAPE
                     LPDATWREIINIDVMVAPGWDGHVPLRLTRLGGRLAGATLRSPRETRHLIHVARTAEG
                     SERPVEQLYYWLPLAGRSWDVVYFPWNSAAIAYRPLMDMRPSVISCRGAQINIAPLNP
                     ERASLRRGLADTFARASAVHCVSDAIRHEAAHYGLDMAKATVIRPAVDPSAFRPAERG
                     LRSSGAPFRIITTGGIIWRKGLEYALLAVRQLRDHGVPVRFDIIGDGPEMQRLLYTIH
                     DLELEACVVRHGRLKPPDVAARLAEADVFLLTSLSEGIANAVLEAMACGLPVVTTDVG
                     GMAEAISDGVEGFLVPPRDDQATAAALLRLYEEPELGRRMGAAARRRIEADFDLRNQI
                     DAFSALFRSVTLV"
     gene            2359..>2791
                     /locus_tag="PLC06_15335"
     CDS             2359..>2791
                     /locus_tag="PLC06_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91871.1"
                     /translation="MRLVLVVSRFPQLSETFIVSQFAGLVDAGWDVHVVCSEEGAWED
                     FPQLATRPELRGRVHRQWPHDAMWKVALLWPLVLVITFMRAPRATIRYWREGLRRFGR
                     RVARRFYLDAVIIALAPDMIHFEFGSQAVGRTDLGELLGCRL"
BASE COUNT          421 a          832 c          953 g          585 t
ORIGIN      
        1 ttggcgatgt gatgagtggc ctgacggccg atggccgcat cgcggtgcct gtctccggcg
       61 gactggactc gcgttcaacg gtggccacgc ttggccgaca accgggaaag atttcgccga
      121 cggatgaggg cctgtgggct tactcctatg gctacggcga cgactctatt gagacgcgca
      181 tcgccgggga actcgccaca cggcgcggct tgcgcttcga tgccttcacg attcgccctt
      241 accttttcga ccgaatcact ccggtaatcg gcgctgttga gggcttccag gatatgacgc
      301 aggcccggca ggcgttcatc ggccgcgagc tacgcgcgaa cgcggacgcc gtgatcgccg
      361 ctcattgggg tgatgtgtat ctgggagaca tgggattggc cgcgacccca cccggcgctc
      421 tgtccggcga ggcgattcgg gaacacgccc tgcacaagat gcgcaagccc ggcggttggc
      481 tcatcgacac ggtttgccgg ccgcatctag ggaaagtcga gccggaggag ttacttcatt
      541 cctttgttgc tgagggtatg gacgggctgg aagatattgc cgacgctgat ttccgcgtca
      601 aggcattcaa gaccgagcag tggtcggcgc gttggacgac ggccagcctg cggatgtatc
      661 aggccgccgt ttggccgcgc ttgccgttct acgacacgcg cctgggtgat ttcttcgcca
      721 ctgtgccgac agagttcgtg gccggccgcc ggttgcagat tgactacctc aagcggttcg
      781 cgccggatct ggcggccgtg ccgtggcagg agtcgggatc aagtctgttc aaggtcggcc
      841 aacccgatcc gatggcggtg gttcgtcgcg ggttcaacaa gggccggcgc atcctgaccg
      901 ggcgtcgagt catcgagagc aactgggaag tgcaattcct gaacgagagc ggccgccacg
      961 ggctggagca atggttgttg cgccgcggct tgcggctgca tgagtttgtc gcgccgcccc
     1021 gaatcgaggc gctcctcact gcgttccagt ccacgcccag ggtggacaaa cgcagctaca
     1081 ccgtttccat gctgctcacg ttttctgcat ggctggagtc gtttggggga ggggcagacg
     1141 ctccatgaac ggccgcgttg atgccccgtt gcgcgtcctc gtgcccggcc tgacatggcc
     1201 gccggagaca tttctggccc ggttgttccg gggcctggcc gcgcggggga ttcagctcac
     1261 gctgatcgcg ccggagctgc ccgacgccac gtggcgagag ataatcaata ttgatgtgat
     1321 ggtcgcgccg ggctgggatg ggcacgtacc gttacgactg acgcgcctgg gtggccggtt
     1381 ggccggcgcc actttgcgtt ccccgcgcga gacgcggcat ctaatccacg tggcacgcac
     1441 cgccgagggt agcgagcgcc ctgtcgagca gttgtattac tggctgccgc tggccgggcg
     1501 cagctgggat gtcgtctatt tcccctggaa ttcggcggcc atcgcctacc ggccgttgat
     1561 ggatatgcgg ccgtcggtca ttagttgccg cggggcgcag attaatattg ccccgctgaa
     1621 cccggagcgc gcctcattgc ggcgcgggct ggccgacaca ttcgcccggg cgtcggccgt
     1681 gcattgtgtg tccgacgcca tccggcatga agcagcccac tatgggctgg atatggcgaa
     1741 agcgactgtt atccggcctg ccgtcgatcc gtcggcattt cgcccggccg aacgcggctt
     1801 gcgctcgtcc ggcgcgccgt ttcgaatcat cacgaccggc gggatcatct ggcgcaaggg
     1861 gctggagtac gcgcttctgg ccgtcaggca gctgcgcgat catggcgtcc ccgtccggtt
     1921 cgatatcatc ggtgacgggc cggagatgca gcggctgctg tatacgatcc acgacctgga
     1981 gcttgaagcc tgcgtggttc gccatgggcg cttgaaaccg ccggatgtgg cggcacggct
     2041 ggccgaggcc gatgtgtttt tgctgaccag tctgagcgag ggcattgcca acgctgtgct
     2101 ggaggcgatg gcctgcgggc tgcccgtggt cacaactgat gtcggcggga tggctgaggc
     2161 gatcagcgac ggtgttgagg gttttttggt gccgccgcgc gatgatcagg ccacggccgc
     2221 cgcgctgttg cgtctctatg aagagccgga gcttggccgc cggatgggcg cggccgcccg
     2281 ccgtcgcatt gaggccgatt tcgatctgcg caaccagatc gacgccttca gcgcgctgtt
     2341 caggagcgtg accctggtat gaggctcgtc ctggttgtct ctcgctttcc gcaattgtcg
     2401 gaaaccttca tcgtgagcca gttcgccggc ctggtcgatg ccgggtggga tgtccatgtc
     2461 gtttgttccg aagagggagc atgggaagat tttccacaat tggccacgcg accggagttg
     2521 cgcggccgtg ttcatcgcca gtggccgcat gatgccatgt ggaaggtggc gctcctgtgg
     2581 ccgttggtat tagtcatcac tttcatgcgc gcgccacgag ccacgattcg ctattggcgc
     2641 gagggtttgc ggcgtttcgg tcgccgtgtg gctcgacgct tttatcttga tgcggtcatc
     2701 atcgcgttgg cacctgacat gatccatttt gagttcggct cgcaggctgt cggccgcact
     2761 gacctgggcg aattgctcgg ttgccgcctg a
//
LOCUS       DAOMQG010000417         2789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14579_length_2789_cov_4.635333, whole genome shotgun sequence.
ACCESSION   DAOMQG010000417 DAOMQG010000000
VERSION     DAOMQG010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14579_length_2789_cov_4.635333"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2789
                     /locus_tag="PLC06_15340"
     CDS             <1..>2789
                     /locus_tag="PLC06_15340"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRO91872.1"
                     /translation="SFSLGLDGPALLVLDDAHLAIDAPEIAHLIDRLVALAPPDLHII
                     LTGRQPLSLPNLSRWKVQGMVRQIGRRDLAFTAVEIAALFTDTYGYALTAEEAGLLHE
                     ATEGWAIALQLVWQSLRSGAYDSVEDVLSSQTTSLDTLFNLLATDIFEGQPADVQAFL
                     RATSVLRVITPEAAGALQIGPGVDTRAMPAYLRRIDLFVVDLGDGSLRYHHIFHQFLE
                     QLTDPEQRLRSHILAAGYYLARGDSDEAIYHLLRAGDREGSASLLDAYGAQLLAGGRL
                     DTLAAYLAGVAEVSGPEILQNHPSLLFYLGELARLRSRFDEALGWYQQAEAAWRDRGQ
                     TEGVSRALRGQARVYLDTVNPSRAEALLEKSIRLSEGLESTEMRARLYELLAENKLNA
                     GQIDEAEALRKQAGEWRSAGPSDSQLLYRVLLRTGRLDEARRKLEERAAAERLEPVLT
                     PRAHRETHLLLSLVCALQGLGEQAHEAAQIGARRGVDLQSPFMIAVGHLRLGHAIALL
                     DDPHRYEHAREQYEQANAISQEIAVPRLSVEAGWGLARVHGYSGDLPRAMQVAAAGIE
                     IAQAAGDEWVASMTRVSLGAGLALAGRYEEAQTWLSRALQGFQECSDTFGACAARLWL
                     CDVYGRQGKVDLLAQWLPVMLAAAEENGYDFLLTRPTLLGPPDERRFVPLLILARDRN
                     WRGDYAARLLAALGLARISRHPGYRLRVRALGEFAAFRGETAIPPNSWRRAKARQLFQ
                     LLITFRHASLDRDQILEYLWPGTDPTAAGRNFKVTLNSLYHVLEPERAAGEESAIIFR
                     DGSVYGIRPGADIELDAERFAALVAQANAAHARRPASAVPLLEAALDLYRGDYLPEAL
                     YETWAAAERERLAVLFLSAADRLAALYLEDGRYDRAIEVVHRLLAADDCWERAWRYLM
                     LACHGLGDRGQIARV"
BASE COUNT          400 a          933 c          995 g          461 t
ORIGIN      
        1 agcttcagtc tcggcctcga cgggccggcc ctgctggtgc tcgacgacgc ccatttggct
       61 atcgacgcac ctgagatcgc ccacctgatc gaccggctgg tggccctggc cccgcccgat
      121 ttgcacatca ttctgaccgg ccgtcagcct ctttcgctgc ccaacctgtc gcgctggaag
      181 gtgcagggaa tggtcaggca aatcggccgc agggacctgg ccttcacggc ggtcgagatc
      241 gccgccctct tcaccgacac ttacggttac gcgctgacgg ccgaagaagc tggcctgctt
      301 catgaggcga ccgagggctg ggccatcgcc ttgcaactgg tgtggcaaag cttgcgcagc
      361 ggggcgtatg attcggtcga ggacgttctc tccagccaga ctacatcgct ggatacactg
      421 ttcaacctgc tggcgacgga tatctttgag gggcaaccgg ccgatgtgca ggcgtttctg
      481 cgggccacgt cggtgttgcg cgtcatcacg cccgaggccg ccggcgcgct gcagattggc
      541 cccggcgtcg atacccgggc catgccggcc tatctgcggc gcatcgacct gttcgtcgtc
      601 gatctgggcg acggcagtct gcgctatcat cacatcttcc accagttcct ggagcagctg
      661 accgaccccg aacagcgcct gaggagccac atcctggccg cgggctacta tctggcgcgc
      721 ggcgattcgg acgaggccat ctatcacctg ttgcgggcgg gcgaccgcga ggggtcggcg
      781 tcgctgctcg atgcctatgg cgcgcaactg ttggccggcg ggcggctcga tacgctggcg
      841 gcctatctgg ccggcgtggc cgaggtctcc gggccggaga ttctgcagaa ccatccgtcg
      901 ctcctgttct atctggggga gctggcccgc ctgcgcagcc ggttcgatga ggcgctgggc
      961 tggtatcagc aggccgaggc cgcctggcgc gaccggggcc agaccgaagg cgtcagccgg
     1021 gcgctccgcg gccaggcccg cgtctacctc gacaccgtga atcccagccg ggccgaggcg
     1081 ctgctggaga agtccattcg cctgagcgag gggctggaaa gcaccgagat gcgcgcccgc
     1141 ctctatgagc tgctggccga gaacaagctg aacgcgggcc aaatcgacga ggccgaggca
     1201 ctccgcaaac aggccggcga atggcgcagc gccgggccgt ccgattccca gctgctctat
     1261 cgggtgctgt tgcgcaccgg acggctggac gaggcccgtc gcaagctgga agagcgggcc
     1321 gcggccgagc gccttgagcc ggtattgacc ccgcgcgccc accgcgagac gcacctgctg
     1381 ctgtcattgg tctgcgcgct gcaggggttg ggcgagcagg ctcatgaggc cgcccaaatc
     1441 ggcgcgcggc gcggggtcga tctgcaatcg ccgttcatga tcgccgtggg ccatttgcgg
     1501 ctgggccacg ccatcgccct gctcgacgac ccccaccgct atgaacatgc ccgcgagcaa
     1561 tatgagcagg ccaacgccat cagccaggag atcgccgtgc cgcgcctgtc ggtggaagcc
     1621 ggttgggggc tggcgcgcgt tcatggctac agcggcgacc tgccgcgggc catgcaggtg
     1681 gccgccgcgg gcatcgagat cgcccaggcg gccggcgacg agtgggtggc gtcgatgacc
     1741 cgcgtgtcgc tgggggcggg gctggcgctg gccgggcgct atgaagaggc ccaaacctgg
     1801 ctcagccgcg cgctgcaggg gttccaggag tgcagtgata ccttcggcgc gtgcgcggcc
     1861 cggctgtggc tgtgcgacgt ctatgggcgg cagggcaagg tcgatctgct cgcccaatgg
     1921 ctgccggtca tgctggccgc ggccgaagaa aacggctacg acttcctgct gacccggccg
     1981 acgctgctcg gcccgcccga cgagcgccgc ttcgtcccgc tgctcattct ggcccgcgac
     2041 cgcaattggc gcggcgacta tgcggcgcgg ctgctggcgg cgctggggct ggcgcgcatc
     2101 agccgccacc ccggctaccg gctgcgcgtg cgggcgctgg gcgagtttgc cgcctttcgc
     2161 ggcgagacgg ccatcccgcc caacagctgg cggcgggcca aggcgcggca gctgttccag
     2221 ctgttgatca ccttccgcca cgcgtcgctc gaccgcgacc agattctcga atatctgtgg
     2281 ccggggacag acccgacggc cgcggggcgc aatttcaagg tgacgctcaa ttcgctctat
     2341 catgtgctgg agccggagcg cgcggcgggc gaggagtcgg cgatcatctt ccgcgacggc
     2401 agcgtctacg gcattcgccc gggggccgat atcgagctgg atgccgagcg gttcgcggcg
     2461 ctggtggccc aggccaacgc ggcccatgcc cgtcgtccgg cgtcggccgt gccgctgctg
     2521 gaggcggcac tcgatctcta tcgcggcgac tatctgccgg aagcgctcta tgagacgtgg
     2581 gcggcggccg aacgggaacg gctggcggtg ctgtttctgt cggccgccga ccggctggcg
     2641 gcgctctatc tggaagacgg ccgatacgac cgggccatcg aggtggtgca tcgcctgctg
     2701 gcggccgacg actgttggga gcgggcctgg cgctatctga tgctggcctg tcacggtctg
     2761 ggcgaccggg ggcagatcgc gcgggtcta
//
LOCUS       DAOMQG010000418         2788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14580_length_2788_cov_8.542261, whole genome shotgun sequence.
ACCESSION   DAOMQG010000418 DAOMQG010000000
VERSION     DAOMQG010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14580_length_2788_cov_8.542261"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLC06_15345"
     CDS             <1..798
                     /locus_tag="PLC06_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRO91873.1"
                     /translation="APLLATLILLGGFPFHLWATTMGRHISPIVAALVLGLVQLIVVV
                     FLLSLLDSVPGVRAMGEFQSAIRWSAALTALIGAFAMIRAADWRGAAGGAILLDMGFL
                     LPVVLVPGTDGFMIAIPALIARYLALLLVGLGAVGWLPGRQSVRFSRWSVPLSPILLI
                     YGLLSLVGLPLTPGFAGRWTQLSAVGLSGGMWPSLLLAFALALATAAIIRMARRRLAA
                     TAESATIEGPFSAFEVGLMVSLAATAALVGLFPGLLIGLAARMLGLG"
     gene            805..1359
                     /locus_tag="PLC06_15350"
     CDS             805..1359
                     /locus_tag="PLC06_15350"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRO91874.1"
                     /translation="MAIVWYALHVKPHKERFVYERLQHLEHYSGQVNIADAAQQIELF
                     FPAVRVKPVNPRSAKVRPYFPGYIFVRGDLELLGENAFSWIPGTHGLVRFGDMPAAVP
                     DVLIRELRERLAEIEAAGGLVFDALKPGDLVRITSGPLAGYEAIFDMRLPGKERVQVL
                     LTFLSRHPQRVQLEVSDVEKVKRP"
     gene            1499..>2788
                     /locus_tag="PLC06_15355"
     CDS             1499..>2788
                     /locus_tag="PLC06_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRO91875.1"
                     /translation="MDRKKQLLDKIADRQAVIGIVGLGYVGLPLAVAFAQEGFQVIGV
                     DVDNRKIDTLNQGESYVEDIPAAVLKPLIDSGIIRATTDYAAMAAADAISICVPTPLR
                     KTKDPDISYIIHAADCLATHMPSGQGQLITLESTTYPGTTDEIVLPRFNRDGLEVGRD
                     FFMAFSPERIDPGRTDYTVRTTPKVIGGVTPGCLEVAVALYSTVVDNPVAVSSTATAE
                     MVKLLENTFRAVNIGLVNEVALMCDKLGLNVWEVVGAAATKPYGFMPFYPGPGLGGHC
                     IPIDPHYLSWKLRTLNYTARFIELAAEVNSHMPDYVLGKVGDALNDERKALNGSRILV
                     LGAAYKRDVGDVRESPALDVIHLLRGRGADVVYNDPYIPTMFLDGDELSSVELSAETV
                     AAADCVVVVTDHTVYDWEWLTTNARLIVDTRNAVGSSG"
BASE COUNT          472 a          837 c          852 g          627 t
ORIGIN      
        1 gctccgctgc tggcgacgct catccttctg ggcggcttcc cgttccacct ttgggccacc
       61 accatgggtc gccacatctc ccccatcgta gcggcgctgg ttttggggct ggtgcagctg
      121 attgttgttg tctttctgct ctctctgctc gattccgtgc cgggagtgcg ggcgatgggt
      181 gagttccagt cggcgattcg ctggagcgcg gcgctaacgg cgttgatcgg tgcgtttgcg
      241 atgatccgcg cggccgattg gcggggcgct gccggcggag caattctgct cgatatgggc
      301 tttctcttgc cggttgtcct cgtacccggc acggacggct ttatgatcgc gattccggcg
      361 ctcattgccc gctatctggc cttgttgctc gttggtctgg gggcggtggg ctggctgccg
      421 ggcaggcaat cggtacgctt tagccgctgg tcagttccac tttcccccat attgttgatc
      481 tacggcctgc tttcgctcgt aggtctgccc ctgacgccgg ggttcgcggg gagatggacg
      541 caactgtcgg ccgtcgggtt gagcggcggc atgtggccgt ccctgctcct cgccttcgca
      601 ttggctttgg ccacggcggc gattatccgc atggcgcgcc gtcggttggc tgcgacggcc
      661 gaatcggcta ccatagaggg acctttctct gcctttgagg tcggcctgat ggtatctctc
      721 gcggccactg ccgcgctggt tggcctgttt cccggcttgc tgatcggcct tgccgctcgc
      781 atgctcgggt tggggtaatt cgcgatggcg atcgtctggt atgcgcttca tgtcaaacca
      841 cacaaggagc ggttcgttta tgagcgcctg caacacctgg aacattattc cgggcaggtg
      901 aacatagcgg atgccgcgca acagatcgag ctgttcttcc ccgccgttcg cgtcaaaccc
      961 gtcaatcccc gttcggccaa ggtgcggccc tattttcccg gctatatttt cgtgcggggt
     1021 gatctggaac ttctgggcga aaacgccttc agctggatac caggcaccca tggcctggtt
     1081 cgcttcggcg acatgccggc tgctgtccct gatgtcctta ttcgcgagct acgagaacgg
     1141 ctggcagaga tcgaggccgc cggcgggctg gttttcgatg cgctgaagcc gggcgatctc
     1201 gtgcgcatta cgtccggtcc tctggccgga tatgaggcta tcttcgatat gcgcctgccc
     1261 ggcaaggagc gcgtgcaggt gctcctcacc ttcctcagtc gtcacccgca gcgagttcag
     1321 cttgaagtca gtgatgtgga aaaggtcaaa aggccgtgaa atcctgcccg tagagcgtta
     1381 taaaacaccc atcctgatgt attattgttg acggtatgag ggacgatgcc cgtcagaata
     1441 gtgttcgcga cggcaatttt cctgtcggcc tgaaactcat cgactacaag gatcaacaat
     1501 ggatcgcaaa aaacagctgc ttgacaaaat tgccgaccgc caggcggtaa tcggtatcgt
     1561 gggcctgggc tacgtaggct tgccgttggc cgtggccttc gctcaggaag gctttcaggt
     1621 cataggcgtt gatgttgaca ataggaagat agacacgctc aatcagggtg agagctacgt
     1681 cgaagacatc cccgcggccg ttttgaagcc gctgatcgat agcgggataa tacgcgccac
     1741 gacggactac gccgcgatgg ctgcggccga cgccatttcc atctgcgtgc ccacgccgct
     1801 gcgcaagacc aaggaccccg atatttccta tatcatccac gccgccgact gtctggcgac
     1861 ccacatgccg tcgggacaag gccaactcat cactctggag agcacgacct atcccggcac
     1921 gaccgacgag attgtcttgc cccggtttaa tcgcgacggt ctggaagtcg gccgtgactt
     1981 cttcatggct ttctcgcccg aacgcattga tcccggtcgc accgactaca cggtgcgcac
     2041 tacgcccaag gtgattggcg gtgtcacgcc cggctgcctt gaggtggccg tcgccctcta
     2101 cagcacggtg gtcgacaatc ctgtagcagt atcaagcacg gccacggccg agatggtgaa
     2161 gttgctggag aacaccttcc gcgccgtcaa tatcggcctg gtcaacgaag ttgccctgat
     2221 gtgcgacaag ctgggcctga atgtgtggga agtggtgggc gcggcagcta ccaagcccta
     2281 cggcttcatg cccttttatc caggccccgg cctgggcggt cattgcatcc ccattgaccc
     2341 ccactatctg agctggaaac tgcgcacgct gaactacacg gcgcggttta tcgagctggc
     2401 ggccgaggtg aacagccata tgcccgatta tgtcctcggc aaggtaggcg acgcgctcaa
     2461 tgacgagcgc aaggcgctaa acggcagccg catcctcgtc ctcggcgcgg cctacaaacg
     2521 tgacgtcggc gacgtgcgcg aaagccccgc actggacgtc attcacctgc tgcgcggtcg
     2581 cggcgccgat gtggtctata acgatcccta tatcccgacc atgttcctgg acggtgatga
     2641 gttgtcgtcg gtggagctat cggccgaaac ggtggccgct gccgattgtg tcgttgtcgt
     2701 caccgaccac accgtctatg actgggagtg gctgacgact aacgcccggc ttatcgtcga
     2761 cacgcgtaat gcggttggtt cttcgggg
//
LOCUS       DAOMQG010000419         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14614_length_2783_cov_3.619868, whole genome shotgun sequence.
ACCESSION   DAOMQG010000419 DAOMQG010000000
VERSION     DAOMQG010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14614_length_2783_cov_3.619868"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLC06_15360"
     CDS             <1..351
                     /locus_tag="PLC06_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRO91876.1"
                     /translation="WGFIEELGLGIDQMIEEMLQAGHPPPEFRATPHSFTVVLSNKRD
                     RPSTPKWTRSMNERQTRAVNFVRESGSITNREYRQLCPDVSPETLRLDLADLVDRGVL
                     LKIGSKKGTYYILK"
     gene            437..>2783
                     /locus_tag="PLC06_15365"
     CDS             437..>2783
                     /locus_tag="PLC06_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO91877.1"
                     /translation="MSFTTPAALLLLLLLPLMAGLVWPRRPGARPLSGWAGLALRLVI
                     VALVVLSLAGAQRVRAVDGLAVVFLVDVSDSMGPENAAAAEQFVRDAVTQMGPDDRAA
                     VVLFGANALVEQPLRDAAELPPFASQPARIATDLAAAIRLGLALLPPDAARRLVILSD
                     GADTVGGPARVGEAARVAAASGVSIDTIHLSRPPAEGEIVLRSVRAPARVGQGESFRL
                     EVSAESAAAGEALLRVLGDGSVIHEETVRLQPGANNFAIRLQAGDPAFARYRVQLSPL
                     GAGDVYPQNNELAAFTEIVGQPRVLVVAADVDKEGQPVDEAAQLAEALAASGLIVERA
                     TPAALSPDLGDLADYAAVVLVNVNARDLSPRKMTALQTYTRDLGGGLVVVGGPDSYGV
                     GGYFGTPLEEVLPVEMQIHDQERFPAVSMALVIDRSGSMSATEGGATKIQLAAEGAIR
                     ALELLNDFDEMTLITVDERPDAIIGPITAANRDEAIVQMRQLGAGGGGIFVRAGLEAA
                     AEALAGSENPVKHVIVLADGSDAEQKEGVPELIEQLTAEGVTVSMISIGAGPDTPWLR
                     EMADLGGGRFHLTDQAANLPQIFTQETVAIQRNYVVEERFFPTQAAEHPILTGIEATP
                     PLYGYVATSARAVAQTPLLTHLGDPLLATWQYGLGRSVAWTSDATGRWAQDWVRWDGF
                     GPFWNQAVRWSFGARRAEGLSASVTFDGEAARLVVDAQRDGEFLTDLTLTANVVGPTG
                     ETQTVELRQTAPGRYEGTLAPTGEGAYFIGIGEITNYELRIT"
BASE COUNT          417 a          943 c         1009 g          414 t
ORIGIN      
        1 tggggcttca tcgaggaact ggggctgggc atcgaccaga tgatcgagga gatgttgcag
       61 gccgggcatc cgccgccgga gttccgcgcc acgccccact cgttcaccgt ggtgctgtcg
      121 aacaagcgcg accggccgag cacgcccaag tggacgcgca gcatgaacga gcgccagacg
      181 cgggcggtca acttcgtgcg cgagtcgggc agcatcacca accgcgaata ccgccaactc
      241 tgccccgacg tgtcgccgga gacgctgcgc ctcgacctgg ccgacctcgt cgaccggggg
      301 gtgctgttga agataggctc gaagaagggc acgtattaca tactgaaatg atgggcttca
      361 cgctacttat cccaaattta tcccaacttt ggatttggga taagttggga tacacgggat
      421 aacccacgtt gagaatatga gcttcacgac ccccgccgcg ctgctgttgc tgctcctgct
      481 gccgctgatg gccgggctgg tttggccgcg gcggccgggc gcgcggccgc tttccggctg
      541 ggcggggctg gcgttgcggc tggtcatcgt cgccctggtc gtcctcagcc tggccggggc
      601 gcagcgcgtc cgcgccgtcg atgggctggc cgtcgtcttt ctggtcgatg tgtccgactc
      661 gatggggccg gagaacgcgg ccgcggccga gcagttcgtg cgcgacgccg tgacccaaat
      721 ggggcccgac gaccgcgccg ccgtcgtcct cttcggcgcc aacgccctcg tggagcaacc
      781 gctgcgcgac gcggccgagc tgccgccgtt tgcctcgcag ccggcgcgca tcgccaccga
      841 tctggccgcg gccatccggc tggggctggc cctgctgccg cccgacgccg cccggcggct
      901 ggtcatcctg agcgacgggg ccgataccgt cggcgggcct gcccgcgtgg gcgaagccgc
      961 ccgcgtggcg gccgcgtccg gcgtatcgat cgacaccatc cacctgtcgc gccccccggc
     1021 cgagggggag atcgtcctgc gctccgtccg cgctccggcg cgcgtgggcc agggggagag
     1081 cttccggctg gaggtgagcg ccgagagcgc ggccgccggc gaggccctgc tgcgcgtgct
     1141 gggcgacggg agcgtcatcc acgaggagac ggtgcgcctg caaccgggcg ccaacaactt
     1201 cgccatccgc ttgcaggcgg gcgatccggc cttcgcccgc taccgggtgc agctctcccc
     1261 gctcggcgcg ggcgacgtct acccgcagaa caacgaattg gccgccttca cggagattgt
     1321 cgggcagccg cgggtgcttg tcgtcgccgc cgacgtcgat aaagaggggc agccggtgga
     1381 cgaggccgct caactggcgg aggcgctggc cgcgtcgggc ctgatcgtgg agcgggccac
     1441 cccggccgcg ctctcgcccg acctgggcga cctggccgac tacgccgccg tcgtgctggt
     1501 caacgtcaac gcccgcgacc tgtcgccgcg caagatgacg gcgctccaga cgtacacccg
     1561 cgatctgggc ggcgggctgg tggtcgtggg cgggccggac agctacggcg tgggcggcta
     1621 tttcggcacg ccattggaag aagtgctgcc ggtggagatg cagatccacg atcaggagcg
     1681 cttcccggcg gtcagcatgg ccctggtcat cgaccggtcg ggcagcatga gcgccaccga
     1741 gggcggcgcc accaagattc aactggcggc cgagggggcc atccgggcgc tggagctgct
     1801 caacgacttc gacgagatga cgctcatcac cgtcgacgag cggccggacg cgatcatcgg
     1861 ccccatcacc gccgccaacc gggacgaggc catcgtccag atgcggcaac tgggcgcggg
     1921 cggcggcggc atcttcgtcc gcgccgggct ggaggcggcg gccgaggcgc tggccggcag
     1981 cgagaacccg gtgaaacatg tgatcgtgct ggccgacggg tcggacgcgg agcagaagga
     2041 gggcgtgccg gaactgatcg agcagctgac ggccgagggg gtcaccgtgt cgatgatcag
     2101 catcggcgcg gggccggaca cgccctggct gcgggagatg gccgatctgg gcggcggccg
     2161 cttccatctg accgaccagg ccgccaatct gccgcagata ttcacccagg agacggtcgc
     2221 catccagcgc aactacgtcg tcgaggagcg ctttttcccc acgcaggcgg ccgagcatcc
     2281 catcctgacc ggcatcgagg ccacgccgcc cctctacggc tacgtggcca ccagcgcccg
     2341 cgccgtggcc cagacgccgc tgctgaccca tctgggcgac ccgctgctgg cgacgtggca
     2401 atatggcctc ggccgcagcg tcgcctggac gtcggacgcc accggccgct gggcgcagga
     2461 ctgggtgcgc tgggacggct tcggcccgtt ctggaatcag gcggtgcgct ggtcgttcgg
     2521 cgcgcgccgg gccgaggggc tgtcggcttc ggtgacgttc gacggggagg cggcgcggct
     2581 ggtcgtcgat gcccaacgcg atggcgaatt cctcaccgat ctgacgctga cggccaacgt
     2641 cgtggggccg acgggcgaga cgcaaacggt cgagttgcgc cagaccgcgc cgggccgcta
     2701 tgaggggacg ttggcgccga caggcgaagg cgcctatttt atcgggatcg gggaaattac
     2761 gaattacgaa ttacgaatta cga
//
LOCUS       DAOMQG010000420         2769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14701_length_2769_cov_8.420413, whole genome shotgun sequence.
ACCESSION   DAOMQG010000420 DAOMQG010000000
VERSION     DAOMQG010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14701_length_2769_cov_8.420413"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..528)
                     /locus_tag="PLC06_15370"
     CDS             complement(31..528)
                     /locus_tag="PLC06_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRO91878.1"
                     /translation="MRIISYTYSLMKRTLYLFICTALLLVTGCRASSATVPAPTAAAD
                     GPPAFDPQQVARGAEIYTLHCAECHGANLEGEADWKIQNPDGSFRAPPHDANGHTWHH
                     SDAQLIDAVRLGGARLSPDVGGTSPMPAYGDTLTDEEITAVLDYIKSTWPEDIRLYQW
                     ERTTQ"
     gene            complement(600..872)
                     /locus_tag="PLC06_15375"
     CDS             complement(600..872)
                     /locus_tag="PLC06_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRO91879.1"
                     /translation="MYIIIKRRLNDYNRWKEIVAEKDGLRAKYGSKGMTSYRNAADPD
                     EVYLIFEWEDEKPYTDYLNLPEVREALGKTGTFEIIEISETFHVTE"
     gene            complement(1023..2045)
                     /locus_tag="PLC06_15380"
     CDS             complement(1023..2045)
                     /locus_tag="PLC06_15380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO91880.1"
                     /translation="MFPQPGEIVTLPESPNIPGLIFRRFRGESDYPGIAAVDNTSREV
                     DGLEWVLTVDDIAREYAHLVNSDPLRDMIIAEINGEIIGFSRGEWLLEASGLYRYLVP
                     VLMSSVWRGRGIRRAMLRWMEARMRQVAAKHPTEARKEFSMWTGPTADSLAALLEGEG
                     YRPARYFNKMTRSLEEALPDFPMPSGLEMRPVRPEHYRLIWDAAEEAFRDHWGYSPSN
                     GEYYQEWLEDPVIFQPELWQIAWNIERNEIAGQVRTFIDELENEKHNRRRGYTEFISV
                     RRPYRRRGLARALIAESLRVQRAHGMTESALTVDTENLTGATRIYEDCGFRTINFSTS
                     YRKPLD"
     gene            complement(2184..>2769)
                     /locus_tag="PLC06_15385"
     CDS             complement(2184..>2769)
                     /locus_tag="PLC06_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91881.1"
                     /translation="GTALQTLLDYAPRYAAVAQRAGIPFTPPGSTAGFMVTERLPGNA
                     TTDYGVPGMIPMSDRQPLDSAELTRHGALLAGCWAVYDTIAAAAVGVELRKGPRGGGR
                     ELEAITRHVFGAEQSYLSSFGWKMPKYMTSDLSEEVRLTRQAIMDGLAASMRGEIAAH
                     GPRGGVRWPARYFIRRVAWHVLDHAWEIADRSSR"
BASE COUNT          539 a          800 c          826 g          604 t
ORIGIN      
        1 cttgacgagc tattgaagct tcaacggccg ctattgcgtc gtccgctccc actggtagag
       61 gcggatatct tccggccagg tgctcttgat gtaatcgagc acagcggtga tttcctcgtc
      121 ggtcagggta tcgccgtagg caggcatggg gctggttccg ccgacgtcgg gagagaggcg
      181 cgccccgccg agccgaacgg cgtcgatcag ctgggcgtcg ctgtggtgcc aggtatggcc
      241 gttggcgtcg tggggcggcg cgcggaacga gccgtccggg ttttgtattt tccagtcggc
      301 ttcaccttcg aggttcgcgc catgacattc ggcgcaatga agggtgtaga tctccgcgcc
      361 acgggcgacc tgctgcgggt cgaaggcggg cgggccgtcg gccgccgcgg tgggcgcggg
      421 gacggtggcg ctgcttgccc gacagccggt caccagcaac aaggccgtac agataaataa
      481 gtagagcgtg cgtttcatca agctataagt atagctaatg atacgcaaga tcaaatgcgc
      541 caaatggcgc tgtttcacga cctaaaatgg tggctgagca ttggccgatt attcggtcat
      601 tattcggtta catgaaaggt ctcgctgatc tcgataatct caaacgtccc ggtcttgccc
      661 agcgcctcgc gaacttccgg caaattcaga tagtctgtat acggtttttc atcttcccac
      721 tcgaaaatga gataaacctc gtccggatcg gcggcgttgc ggtatgacgt catgcctttc
      781 gagccatact ttgcgcgtaa tccatccttt tcggccacaa tctctttcca acggttgtag
      841 tcattgagcc ttcgcttgat gatgatgtac atcttttctc ctcggattga aaggtgtgat
      901 gcaggattgg agaaatctcg acacagtcga ggattctggg cgatcgcgga tcgaataccc
      961 cgattgtacg gtaaattata ttgtaaggat cgtcgtgaac agatcaatac cctctggtcg
     1021 ttctaatcaa gtggcttgcg gtatgaagtc gaaaagttta ttgtccggaa accgcaatcc
     1081 tcatagattc gggttgcccc ggtcaggttt tcggtgtcca cggtgagggc cgattcggtc
     1141 attccgtgcg ccctctgaac gcgcaggctt tcggcaatga gcgcccgggc cagaccgcgc
     1201 cgccgatagg gccgtcgcac gctgataaac tcggtgtagc cgcgccggcg gttgtgtttc
     1261 tcattctcca gttcgtcgat gaacgtgcgc acttgcccgg ctatctcgtt gcgttcaata
     1321 ttccaggcga tctgccacaa ctccggttgg aagattaccg gatcttccag ccattcctgg
     1381 tagtactctc cattgcttgg gctatatccc caatgatcac gaaaagcctc ttcggcagcg
     1441 tcccaaatga gacggtagtg ttccggtctc accgggcgca tctccaggcc ggagggcatg
     1501 gggaaatcgg gcagggcctc ttccagcgaa cgtgtcatct tgttgaagta tcgcgccggc
     1561 cggtagcctt cgccttccag caaggcggcc agactatctg ccgtgggtcc cgtccacata
     1621 gaaaactctt tgcgtgcctc ggttggatgt ttggcggcaa cttgacgcat gcgtgcttcc
     1681 atccagcgca gcatggcccg gcgtatcccc cgcccgcgcc acacagacga catcaggacg
     1741 ggtaccaggt agcgataaag gccggacgct tccagcaacc attcgccacg ggaaaagccg
     1801 attatctcgc cgttgatttc ggcgatgatc atatcccgca gcggatcgct gttgaccagg
     1861 tgggcatact cgcgggcgat gtcgtcgact gtcagtaccc actccagccc atcgacctcg
     1921 cgtgaggtgt tgtccacggc ggcgatgccg ggatagtcgc tctcgccgcg gaaccgccga
     1981 aagatcaagc cgggtatgtt gggtgattcg ggcagggtta ctatttcgcc gggttgtgga
     2041 aacatggctt tcgctccgtt gctgctttga atttgtcgat gtgagagcaa aggaaaacag
     2101 gatagcatga aaacaaaggc caaatcaagg ggcggttgac gctgttcagg aacgatgctt
     2161 gtcctgaagc gcggcgtctc cgactaccgg gatgaccgat cggcgatctc ccaggcgtga
     2221 tccagcacat gccaggcaac gcggcggata aagtagcgcg ccggccatct gacgccgccg
     2281 cgcgggccgt gtgcggctat ctcgccgcgc attgatgccg ccaggccatc catgatagcc
     2341 tgtcgggtta gccggacctc ttcgctcaga tcggatgtca tatatttggg cattttccag
     2401 ccgaacgatg ataaataact ctgctcagcg ccgaacacat ggcgagtgat ggcctccagt
     2461 tcgcgaccac cgccgcgcgg ccccttgcgc aactccacgc cgacggccgc cgcggcgatg
     2521 gtatcgtaga ccgcccagca gcccgccagc agcgccccgt gacgtgtcag ttcggccgaa
     2581 tcgagcggtt gccggtcact catgggaatc attcccggca cgccataatc ggtcgtagca
     2641 tttcccggca agcgctcagt caccatgaag ccggccgttg aaccgggcgg agtaaacgga
     2701 atgcccgcgc gctgggccac ggccgcatag cgcggggcgt agtcgagcag cgtctgaagc
     2761 gctgtgccc
//
LOCUS       DAOMQG010000421         2760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14764_length_2760_cov_7.534196, whole genome shotgun sequence.
ACCESSION   DAOMQG010000421 DAOMQG010000000
VERSION     DAOMQG010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14764_length_2760_cov_7.534196"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLC06_15390"
     CDS             <1..239
                     /locus_tag="PLC06_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91882.1"
                     /translation="ADVPDWLQPLAAKPAPPSTHLTPARPAPGQAQPAAARKPAAVKK
                     KQKNSGNQALSLLLGLLLLLTVLVVAFLIYLLLF"
     gene            complement(243..593)
                     /locus_tag="PLC06_15395"
     CDS             complement(243..593)
                     /locus_tag="PLC06_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRO91883.1"
                     /translation="MKVRFQADADLNQNIVKATLRLEPGIDFQTASAAGLMGLPDLDV
                     LKLAAEQGRILVSHDRKTMPEHFGQFIQSENCPGILIVSQREAVSQVANELLLIWLAT
                     EPDEWVNRLRSLPL"
     gene            complement(590..904)
                     /locus_tag="PLC06_15400"
     CDS             complement(590..904)
                     /locus_tag="PLC06_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO91884.1"
                     /translation="MDRQYVEQRDEGYRISGSRVSLESVIYAFLDGVSPETIVAEYFP
                     VLTLEQVYGAITYYLSHRDELDAYLRQVEAEYDTFRQATIDPAFSRKLTLARRQLRPI
                     EI"
     gene            complement(981..2087)
                     /locus_tag="PLC06_15405"
     CDS             complement(981..2087)
                     /locus_tag="PLC06_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91885.1"
                     /translation="MRLHRWSIFLIIMVMTLTGCRSQSADPAILYLAPGPNGATQLYR
                     LDPGAAAARQLTGLDDPRAPEVIDFAADPDGRRIAYMTQAAEGDSAIRLIDAGGNDKS
                     LAACPQAECSGPVWSPDGRRLIYELRPRESGLLASPRLHWLDPTTGDTLPLIEGNDTP
                     GYGARFSPDGQWLSYVSLADDGVVLYRLSDGRQRLLSSRVGSPAAWSPDSAAVVYGDL
                     VVQGHQTAPEPEGGAGEVPIQESSNIFLYLSPVDEESDRRRLSPDAAVADTAAAFSPD
                     GAWIAFGRAPANTAAGRQLWLMRADGSDARALTDDPAITHGPPRWSPDGRALLFQRYD
                     PAEETTSVWRLDIATGAETRVVEDGYLPAWVGGG"
     gene            complement(2191..>2760)
                     /gene="phoU"
                     /locus_tag="PLC06_15410"
     CDS             complement(2191..>2760)
                     /gene="phoU"
                     /locus_tag="PLC06_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRO91886.1"
                     /translation="MTYAFVERDLVTAQRMIDADQSINERRITIGLDALRLIATQSPM
                     AGDMRMIAAILETVGELERIHDYVKGIGKISLTIGPEPIPAPLAQKLPEMGETAGEML
                     FQALKAFANRDEALARRVPAMDDRVDGLFNELYEAIIAIVLADPPQIQRAGRLEWALH
                     NLERAADRTINICEWVVYLVTGVYAEMDE"
BASE COUNT          482 a          840 c          929 g          509 t
ORIGIN      
        1 cggccgacgt gcccgactgg ctgcagccgc ttgccgccaa gcccgctccg ccgtcgaccc
       61 acctgacccc ggcgcgcccc gctccaggcc aggctcaacc ggcggccgcc cgcaaaccgg
      121 ccgcggtcaa gaaaaagcag aagaacagcg gtaatcaggc actatcgctc ctgctggggc
      181 tattgctgct gctgacggtg ctggtggtgg cgtttttgat ttatctgttg ttgttttgag
      241 gcttacaatg gcaatgagcg gagccgattc acccattcat ccggttccgt cgctaaccag
      301 ataagcagca actcattggc gacctgggaa acagcctctc tttgggagac gatgaggatg
      361 ccgggacaat tctctgactg gatgaattga ccgaaatgtt ccggcatcgt ctttcgatca
      421 tgcgacacca gaatcctgcc ctgctcggca gccaatttca atacatcaag gtcggggagg
      481 cccattaatc cggcagccga agccgtttga aagtcgatgc caggctccag acgcaaagtt
      541 gctttaacga tgttctggtt taaatcagca tctgcctgga accgaacttt catatctcaa
      601 tcggccgcag ttgtcgacgt gccagcgtca gtttgcgaga gaaggcgggg tcgattgtcg
      661 cctgccggaa ggtgtcgtat tcggcttcga cttgccgcag gtaggcatcg agttcatcac
      721 gatggctcaa ataataggta atggccccat atacctgctc cagcgtgagg accgggaagt
      781 attcagccac gatcgtctcg ggggatacgc catccagaaa tgcataaatg acggactcca
      841 atgacacccg cgagccggaa atccggtacc cttcatcccg ctgttctacg tattgtctat
      901 ccatcagtca cgtcctggat atttaattga tcgtacaggt accattatat cccattgaat
      961 gctgagtatt aaaacacaaa tcacccgccc ccaacccacg ccggcagata cccgtcttcc
     1021 acaacccgcg tctccgcgcc ggtggctata tccaaccgcc agacggaggt cgtctcttcg
     1081 gcggggtcgt agcgctggaa gagcagcgcg cggccgtcgg gcgaccagcg cggcgggccg
     1141 tgggtgatgg ccgggtcgtc ggtcagggcg cgggcgtcgc tgccgtcggc gcgcatcagc
     1201 cacagctggc ggccggcggc ggtgttggcc ggggcgcggc cgaaggcgat ccacgcacca
     1261 tcgggcgaga aggcggcggc ggtgtcggcc acggccgcat cggggctgag ccgccgtcga
     1321 tcgctctcct cgtccaccgg cgacagatag aggaagatgt tggatgattc ctgaatcggc
     1381 acttcccctg ccccgccttc cggctccggc gcggtctggt gtccctggac gacgagatcg
     1441 ccatagacga cggccgcgct gtcgggactc caggcggccg ggctgcccac gcggctgctg
     1501 aggagccgct ggcggccgtc gctcaaccga tagagcacca cgccgtcgtc ggccagcgag
     1561 acgtagctga gccattggcc gtcgggggag aagcgcgcgc catagccggg ggtgtcgttg
     1621 ccctcgatga gcggcagggt gtcgccggtg gtcgggtcga gccagtggag gcggggggag
     1681 gcgagcaggc cgctctcgcg cgggcgcaat tcgtagatga ggcggcggcc gtcgggcgac
     1741 cagaccgggc cggagcattc ggcctggggg caggcggcca ggcttttgtc gttgccgccg
     1801 gcgtcgatga gacggatcgc cgagtcgcct tcggccgcct gggtcatgta ggcgatacgg
     1861 cggccgtcgg ggtcggcggc gaagtcgatc acttcgggcg cgcgggggtc gtcgaggccg
     1921 gtgagctggc gggcggccgc cgcgccgggg tcgagccggt agagttgggt cgcgccgttg
     1981 gggccggggg cgaggtagag gatggccggg tcggccgatt gggagcgaca accggtcagg
     2041 gtcatcacca ttataataag gaagatgctc cagcgatgga gtcgcatggg gtgatcctac
     2101 tcgttgcggg gcggcggggc aagattggag gaaagtacga agtacgaagg aatcgcgcca
     2161 ctttcgtact tcgccttcac tcgtcgctcg tcattcgtcc atctccgcat agacgccggt
     2221 aaccagatag accacccatt cgcagatatt gatcgtgcgg tcggcggcgc gctccaggtt
     2281 gtggagcgcc cattccagcc gcccggcgcg ctgaatctgc ggcgggtcgg ccaggacgat
     2341 ggcgatgatc gcctcgtata gctcgttgaa caggccatcg acgcgatcgt ccatggccgg
     2401 gacgcgccgg gcgagcgcct cgtcgcggtt ggcgaacgcc ttcagcgcct ggaacagcat
     2461 ctcgccggcg gtctcgccca tctccggcag cttttgggcc agcggcgcgg ggatcggctc
     2521 cggcccgatg gtcaggctga tcttgccgat gcccttgacg tagtcgtgga tccgctccag
     2581 ctcgccgacg gtctccagaa tggcggcgat catgcgcatg tcgccggcca tgggcgactg
     2641 ggtggcgatg agccgcaatg cgtcgaggcc gatggtgatg cggcgctcgt tgatcgactg
     2701 gtcggcgtca atcatccgct gcgccgtcac caggtcgcgc tccacgaagg cataggtcat
//
LOCUS       DAOMQG010000422         2757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14793_length_2757_cov_7.854182, whole genome shotgun sequence.
ACCESSION   DAOMQG010000422 DAOMQG010000000
VERSION     DAOMQG010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14793_length_2757_cov_7.854182"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..901
                     /locus_tag="PLC06_15415"
     CDS             41..901
                     /locus_tag="PLC06_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRO91887.1"
                     /translation="MKVGIDSYCFHRYFGEVYGHQTPPPFTMTVFDFLDFAKEMDVDG
                     VSLESCFFPSHDNAFYDDLKARLDGYGFDRVYAWGHPDGLEAGRNEDAFHDMIKSIEY
                     AARIGAPVMRICASSFSYRLEPHAPQMDKLVPWLKEGAKVAQSYGVKLAAENHIDYTS
                     SEWLELLERVDSPYMGLNLDTGNFVRLLDDPIDGARKLADRVYATHIKDLMPARGVNV
                     GEWYFFSSTPVGDGLVDNQQLAQILHDANYQGFLAVETDSLHPAYENQEHWAVEKSVW
                     TLKRIARNVK"
     gene            1135..2358
                     /locus_tag="PLC06_15420"
     CDS             1135..2358
                     /locus_tag="PLC06_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRO91888.1"
                     /translation="MLEPLEAAVVTAIRRRGPLSRSDLSEHLDYSRASVTGIVGRMID
                     AEILAEAGEGKSAGGRRPWLLDINPRLGYVVGVDIGATSVDVALADFRGSILERAGEA
                     ADVRGRPEPFLDHVADLIRELLDRRGAAASEVVGIGVGVPGPVEFSSGVLIAPPLMPL
                     WDDFPIRRYLRDFFPNARVVVDNDVNIMAKGEHQAGAGRDMDNFLFIKIGTGIGCGII
                     SHGEVYRGADGAAGDVGHICVDYNGPVCHCGNQGCLEILTAGPAIAAEALARAESGES
                     PFLAARLAERKKDLTGFPEPVRSEGLLTAIDVGDAAAAGDRVALEIVRRSGRMIGGVL
                     AMLVNFYNPEAVFIGGGVANIGHGLLSAIRQATLRRATALSTRHLRVEYSPLGEDAGV
                     IGGIRLALEHVFVIK"
     gene            2406..>2757
                     /locus_tag="PLC06_15425"
     CDS             2406..>2757
                     /locus_tag="PLC06_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRO91889.1"
                     /translation="MGEYPLRERTKKLALRIIKLVEALPSSETGRIIGRQLLRSGTSV
                     GANYRASLRAKSKADMLNKWKIVGEEADETLYWLELLIEADLMPESKLRPLMNEVEEI
                     LSMLVASIRTLRESG"
BASE COUNT          525 a          878 c          863 g          491 t
ORIGIN      
        1 gtaattcgta attaaacaga aaggagaatc cgcaatggcc atgaaagttg gaatcgacag
       61 ctattgtttt catcgttatt ttggtgaagt gtacgggcat cagaccccgc cgccgttcac
      121 gatgaccgtc tttgacttcc tcgacttcgc caaggagatg gacgttgacg gcgtgtcgct
      181 ggagtcgtgc ttcttcccgt cccacgacaa cgcgttctat gacgatctga aggcgcgcct
      241 cgacggctac ggcttcgacc gggtctatgc ctggggccat cccgacggcc tggaagcggg
      301 gcgcaacgag gacgccttcc acgacatgat caagagcatt gagtacgcgg cccgcatcgg
      361 cgcgccggtc atgcgcatct gcgccagcag cttctcctac cgcctggagc cgcacgcgcc
      421 gcagatggac aagctcgtgc cgtggctgaa ggaaggggcg aaggtggccc agtcttatgg
      481 cgtcaagctg gcggccgaga accatatcga ctacaccagc agcgaatggc tggagctgct
      541 ggagcgggtc gattcccctt acatgggtct caacctcgac accggcaact tcgtgcgcct
      601 gctcgacgac cccatcgacg gcgcgcgcaa gctggccgac cgcgtctacg ccacccacat
      661 caaggacctc atgcccgctc gcggcgtcaa cgtcggcgag tggtacttct tcagcagcac
      721 ccccgtgggc gatgggctgg tggacaacca gcagctggcc cagattctcc acgacgccaa
      781 ctatcagggc ttcctcgccg tggagaccga ctcactgcac ccggcctacg agaatcagga
      841 gcattgggcg gtcgagaaga gcgtctggac gctcaagcgc atcgcccgca acgtgaaata
      901 gttcggcgat ccggccggcg atggaggcac tcgcccccaa tcgccggccg tggtcttttt
      961 tactcattga cagggcactc accttatgct aatctatccg caacttgctt tattaaatga
     1021 aacaagtcgt tcgtattgtg acagaagtca caatgcaggc gacgcgagag accgggagcg
     1081 gatagcgtgg ccgagttgtc gcccatttca gacagtaccg acgagccgaa cctaatgctg
     1141 gaaccacttg aagcggccgt cgtcaccgcc atccgccgcc gcgggccgct ctcccgctcc
     1201 gatctgtcgg agcatctcga ctactcccgc gccagcgtca ccggcatcgt cggccgcatg
     1261 atcgacgccg aaatcctggc cgaagcgggc gagggcaagt ccgccggcgg ccgtcgcccc
     1321 tggctgctcg acatcaaccc ccggctcggc tacgtcgtcg gcgtcgatat cggcgcgacc
     1381 agcgtcgacg tcgccctggc cgactttcgc gggagcattc tggagcgcgc cggcgaagcg
     1441 gccgacgtgc gcggccgccc cgaaccgttc ctcgaccacg tggccgatct cattcgtgaa
     1501 ctgctcgacc ggcgcggcgc ggcggcgagc gaagtcgtcg gcatcggcgt cggcgtgccc
     1561 ggcccggtgg agttctcctc cggcgtcctc atcgccccgc cgctcatgcc gctctgggac
     1621 gacttcccca tccgccgcta tctgcgcgac ttctttccca acgcccgcgt cgtcgtcgac
     1681 aacgacgtca acatcatggc caagggggag catcaggccg gggcaggccg cgacatggac
     1741 aacttcctgt tcatcaagat cggcaccggc atcggctgcg gcatcatctc ccatggcgag
     1801 gtgtatcgcg gggccgacgg cgcggccggg gacgtgggcc acatctgcgt tgactacaac
     1861 ggcccggtgt gccattgcgg caatcagggc tgtctggaga tactgaccgc cggcccggcc
     1921 atcgccgctg aagcgctggc gcgggccgaa tcgggcgaaa gcccctttct ggcggcgcgg
     1981 ctggccgagc gaaagaaaga tctgacaggt ttccccgaac ctgtcaggtc tgagggacta
     2041 ctgaccgcca tcgacgtggg cgacgcggcg gccgccggcg atcgggtggc gctggagatc
     2101 gtgcgccgca gcggccgcat gatcggcggc gtgctggcca tgctggtcaa cttctacaac
     2161 cccgaagcgg tcttcatcgg cggcggcgtg gccaatatcg gtcacggcct gctgtcggcc
     2221 atccgtcagg cgacattgcg ccgggccacg gcgctgagca cccgccacct gcgcgtcgaa
     2281 tactcccccc tgggcgagga cgccggcgtc atcggcggca tccggctggc gctggaacac
     2341 gttttcgtca tcaagtagat acgagatacg agatacgaga tacgagttaa gagggaaggt
     2401 gcacgatggg cgaatatcca ttacgcgagc gcacgaagaa gctggctctg agaattatca
     2461 aactggtgga ggctttgccg tcgagtgaaa ccgggcgcat catcggtcgc cagctgcttc
     2521 gctcaggcac ttcggtgggc gctaactaca gggcgtcgct gcgggcgaaa tccaaagccg
     2581 acatgttgaa caaatggaag attgtgggag aggaagcgga tgagacgctc tactggctgg
     2641 agttgctcat cgaggcggac ctgatgcccg aaagcaaatt gcgcccgctt atgaatgaag
     2701 tcgaggagat tttatcaatg ctcgtcgctt ccatcagaac attacgcgag agcggtc
//
LOCUS       DAOMQG010000423         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14858_length_2745_cov_8.825651, whole genome shotgun sequence.
ACCESSION   DAOMQG010000423 DAOMQG010000000
VERSION     DAOMQG010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14858_length_2745_cov_8.825651"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..616
                     /gene="tadA"
                     /locus_tag="PLC06_15430"
     CDS             140..616
                     /gene="tadA"
                     /locus_tag="PLC06_15430"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRO91890.1"
                     /translation="MDEYERWMRLALAEAGKAMSLGEVPVGAVAVLDGRVIGSGFNRK
                     ESDQDPTAHAEMIALREAAARLENWRLVGVTLYCTLEPCPMCAGAMIQGRLSRLVYGA
                     RDTRFGADGTIIDVLGQPAFNHRVETISGVLAEEAGALLQAFFRMTRNQRRMTSDE"
     gene            719..1909
                     /locus_tag="PLC06_15435"
     CDS             719..1909
                     /locus_tag="PLC06_15435"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRO91891.1"
                     /translation="MLDYIERKLALPGDDELLRKRKVAAFFAGLFGAIVALLFVVLYF
                     SVGDPLPAWLYATTVAWTTVGLIVLWNRPHTYYPVVWLTALYITIHPWIITLFTGGYS
                     SGLLPMLWALLGPGFAVVLVGISPALFNSALYVVLAVITALLDPWAAAHAPELPHWFQ
                     LLAGTLSAVMPGTMIVFVSLFLFRQLERARSQADALLRNILPSPVADRLKVDPTTIAD
                     SFNEVTVLFADLVGFTRMSAAADPRAVVGLLNAVFSEFDALTEKHGLEKIKTIGDAYM
                     VVGGLPVPRPDHVEAVMAFALDALVVLKRYHALDGSPLGVRIGINTGPTVAGVIGRRK
                     FIYDLWGDTVNTASRMEAFGLENVIQVTQAVRDKLQERYTFEERGPIEVKGKGLMTTW
                     LLRP"
     gene            1988..2617
                     /locus_tag="PLC06_15440"
     CDS             1988..2617
                     /locus_tag="PLC06_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRO91892.1"
                     /translation="MPTQPSPNPSRSSQQSLSVLYMSMSLDGYIAGPDDNPTNPGGDG
                     FMRLHDWYGFTGEPPNTKGTGWGEHFIEEGNATGAVLAGRRTVEQVDHWGGNHHGVPI
                     FVVSHRPPDPSVAEKYPLVTYVTDGIESAMTQAKAAAGTRNVMVHGAYTAQVALAAGV
                     LDELQIHQIPVLFGNGRRLFDLLPSRIELAIVRVIDTPEATHIRYRVLP"
BASE COUNT          487 a          850 c          872 g          536 t
ORIGIN      
        1 gggtgattac ccttgccgcg gcgccgggta agaatccggc gttacatcgt agccggctgc
       61 tgaaaaaccg tgtcgtcctt cgtacttcgt acctcgtttt gccacaacac tggtataata
      121 cgccacggcg taatgtagta tggatgaata tgaacgatgg atgcgcctgg cgctggccga
      181 ggcgggaaag gcgatgagcc tgggcgaggt gccggtgggg gcggtggccg tgctcgacgg
      241 ccgcgtcatc ggctccggct tcaaccgcaa ggagagcgac caggacccca ccgcccacgc
      301 cgagatgatc gcgctgcgcg aggcggccgc gcggctggag aactggcggc tcgtcggcgt
      361 gacgctctat tgcacgctgg agccgtgccc catgtgcgcc ggggcgatga tccaggggcg
      421 attgtcgcgg ctggtctatg gcgccaggga tacccgcttt ggggccgacg gcacgatcat
      481 cgacgtgctg gggcaaccgg ctttcaatca tcgggtcgag acaatctccg gcgtgctggc
      541 cgaagaagcc ggcgcattat tacaagcgtt ctttcggatg accagaaacc agagacgaat
      601 gacgagtgac gagtaaagag taacgagtga cgaaggcgac tcccgtcgcc cgacgaatga
      661 ccaaggcgct cttcattcgt acttcgtact tcgtaattcg tacttcgtaa ttaccatgat
      721 gctcgactac attgaacgca aactggcatt gcccggcgac gacgagctgt tgcgcaagcg
      781 caaggtggcg gctttcttcg ccgggttgtt cggggccatc gtggcgctgc ttttcgtcgt
      841 gctctatttc agcgtcggcg atccactgcc tgcctggctc tatgctacca ccgtggcctg
      901 gacgaccgtc ggtctgatcg tgctgtggaa tcggccgcac acctattatc cggtcgtatg
      961 gctgaccgcc ctctatatca cgatccaccc ctggatcatc acgctgttca ccggcggcta
     1021 tagttccggc ctgctgccca tgctgtgggc gctgctgggg ccggggttcg ccgtggtgct
     1081 cgtcggcatc agcccggcgc tgttcaattc ggcgctctat gtggttctgg ccgtcataac
     1141 cgcgctgctg gatccctggg ccgccgccca cgcccccgaa ctgccgcact ggtttcagct
     1201 gctggccggc acgctcagcg ccgtcatgcc gggcacgatg atcgtcttcg tcagcctgtt
     1261 tctctttcgc cagctggagc gggcgcggtc gcaggccgac gcgctgctgc gcaatatcct
     1321 gccctcgccc gtggccgacc ggctgaaggt tgaccccacg accatcgccg acagcttcaa
     1381 cgaggtgacc gtgctcttcg ccgacctggt gggattcacg cggatgagcg ccgcggccga
     1441 tccgcgggcg gtcgtcgggc tgctcaacgc cgtcttctcc gagttcgacg ccctgacgga
     1501 gaagcacggg ctggagaaga tcaagacgat tggcgacgcc tacatggtcg tgggcgggct
     1561 gcccgtgccg cggccggacc atgtggaagc ggtgatggcc tttgcccttg acgcgctggt
     1621 cgtgctgaag cgctatcacg cgctcgacgg cagcccgctc ggcgtccgca tcggcatcaa
     1681 caccggcccg acggtggccg gggtcatcgg ccgccgcaag ttcatctacg atttgtgggg
     1741 cgacacggtc aacacggcca gccgcatgga agcgtttggg ctggagaatg tcatccaggt
     1801 gacccaggcc gtgcgcgaca agctgcaaga acgatataca ttcgaggagc gcggaccgat
     1861 cgaggtgaaa ggcaaggggc tgatgacaac ctggttgttg cggccgtgaa gaggaatgac
     1921 gagtgacgag tgacaaatga agcgcgcgcg cttgcggtct gtcgtctgtg gtccaatata
     1981 acataacatg ccgacccaac catcccccaa cccatcgcgc tcatcgcagc aatctctatc
     2041 tgtgctctat atgtccatgt cgttggacgg ctacatcgcc ggtcccgacg acaacccgac
     2101 caaccccggc ggcgacggct tcatgcggct gcacgactgg tacgggttca ccggggagcc
     2161 gcccaacacg aagggcaccg gctggggcga gcacttcatc gaagagggca acgccaccgg
     2221 cgcggtgctt gccggccgcc gcaccgtcga acaggtcgat cattggggtg gcaatcatca
     2281 tggcgtcccc atctttgtcg tcagccatcg gccgcccgac ccgtcagtgg ccgaaaaata
     2341 tcctttggtg acttatgtga ccgacggaat cgagagcgcc atgacccagg ccaaggctgc
     2401 tgccggaacc cggaatgtga tggtccatgg cgcgtatacg gcacaggtcg cgctggcggc
     2461 gggagtgctg gacgagctgc aaatccacca gatcccggtg ctgtttggca acggccgccg
     2521 tctgttcgat ctgttgccgt cgcgcatcga gctggcgatt gtccgggtta tcgacacgcc
     2581 ggaagccacg catattcgct atcgggttct tccttagaag tacgaaggac ggccgttgac
     2641 ggtcgtcctt cgcacttctt gccatcccgc ccacaaatac gctatactcc ccttgcttgt
     2701 cgaactggag aggtgccgga gcggttgatc ggggcgctct cgaaa
//
LOCUS       DAOMQG010000424         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14955_length_2732_cov_9.565932, whole genome shotgun sequence.
ACCESSION   DAOMQG010000424 DAOMQG010000000
VERSION     DAOMQG010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_2732_cov_9.565932"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLC06_15445"
     CDS             complement(<1..1316)
                     /locus_tag="PLC06_15445"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRO91893.1"
                     /translation="MKSSDFKRINPYLYEIPAAHRPDMRVPARFYADAELLQQALGDK
                     SLDQLANSATLPGIVAYALAMPDIHQGYGFPIGGVVATRVPDGVISPGGVGYDINCGV
                     RLLGTRLSRADIAPYLDELATQLQRNCPHGVGRGGEFVLNRKQLEDVVRKGAGWALER
                     GYATPIDLELTEENGRIEGADPAKISARAYERARDQLGTLGSGNHFIELDVVDDIFDE
                     TAATRMGLFPGQIVAQIHCGSRGFGHQVCTDYVARFQRVVHQYGIVLPDRELVCAPLS
                     SPEGQDYLAAMKGAANFAFANRQLLTHLIRRSFDDVLAGKVDDPSVYQIYDIAHNMAK
                     IEEHEVDGKRVRVCVHRKGATRAFGPGSAVLPDVYRDIGQPVLVPGSMGTASWVLVGT
                     EGSMAQSFGSTCHGAGRVMSRSRAKREVQGDELRRGLESRGIRVRAG"
     gene            complement(1326..1751)
                     /locus_tag="PLC06_15450"
     CDS             complement(1326..1751)
                     /locus_tag="PLC06_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRO91894.1"
                     /translation="MMDAPEDDDIRIVEHTADWALRLRGDDLKALFARAAEGMAYLLA
                     GDTSVVPRDLSRDLTLEAYDAEEMLVLFLGELVYWAERDGLVFPVADLRDVTPTRLVG
                     TVSGGRPAEIQKHIKAVTYHDLAIRQTEAGLEVTVVFDV"
     gene            complement(1771..2601)
                     /locus_tag="PLC06_15455"
     CDS             complement(1771..2601)
                     /locus_tag="PLC06_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRO91895.1"
                     /translation="MADRCPNCDKPILATDVVCWHCGYQLPKRAAAPTPKRAFTKTPD
                     LAAREEDVTPADYNWRALLVYGLLTLAIIAGLWLVMRSLSLRPVLVSGAGFDSDGEWV
                     RVTDADLRYTVSIPIEWQWIDMAYRDQSELLSRVVAQQTYVNRALGPLGELADDVETV
                     GLAVGAGLLDEVDPQPFLLIGRSERLRGVAPQDVLDALDENSFQISEKEINTHLAGQP
                     QARFIALDPSNEYQCRHLFVSGEGKPGYLVAACAPQEHFGALQRDLNVILDSFQLLEY
                     "
BASE COUNT          559 a          908 c          774 g          491 t
ORIGIN      
        1 ccggcgcgca cgcggatgcc gcgactctcc agcccgcgcc gcaactcgtc gccctgcacc
       61 tctcgcttgg cccgtgagcg gctcatcaca cgccccgcgc cgtggcatgt ggagccgaag
      121 ctctgagcca tcgacccttc cgtacccacc agcacccaac tggccgtgcc catcgatccc
      181 ggaacgagca ccggctggcc aatgtcgcga tacacatcgg gcaggacggc cgagccgggg
      241 ccgaaagcgc gcgtcgcccc cttgcgatgg acgcagacgc gcacccgctt gccgtccact
      301 tcatgttcct caattttggc catattgtgg gcgatgtcat agatctgata cacgctgggg
      361 tcgtcgacct tgcccgccag gacgtcatcg aagctgcgac ggatcagatg ggtcagcaac
      421 tggcgattgg caaaggcgaa gttggccgca cccttcatcg ccgccagata gtcctggccc
      481 tccgggctgc ttagcggtgc gcaaaccagc tcgcgatcgg gcaaaacgat gccgtattgg
      541 tgaacaaccc gctggaagcg ggccacataa tcggtgcaaa cctgatgccc gaagccgcgc
      601 gagccgcaat gaatctgggc gacgatctgg ccggggaaga gacccatgcg cgtcgcggcc
      661 gtctcatcga agatatcatc caccacatcc aactcgatga agtggttgcc ggaaccgagc
      721 gtccccaact gatcacgggc gcgttcatag gcgcgagcgc ttattttcgc cggatcggcc
      781 ccttcgatgc ggccattttc ctcggtaagt tccaggtcta tgggcgtcgc gtaaccgcgc
      841 tccagcgccc agcccgcccc cttgcggacc acgtcttcca gttgcttgcg attcagcacg
      901 aactcaccgc cgcggccgac gccgtgaggg caattgcgct ggagttgggt cgccagttcg
      961 tccagatagg gcgcgatatc ggcccggctc aggcgcgtgc ccagcaggcg cacgccacaa
     1021 ttgatgtcat agccgacgcc ccccggcgag atcacaccgt cgggcacgcg cgtcgccacc
     1081 acgccgccga tggggaagcc atatccttga tgaatgtcgg gcatggccag cgcataggca
     1141 acgatgccgg gcagcgtggc gctgttggcc agctgatcga ggctcttgtc gccaagcgct
     1201 tgctgcaaca gctcggcatc ggcgtagaac cgcgccggca cgcgcatatc cggccggtgg
     1261 gcggcaggga tctcgtataa gtaggggttg atgcgtttga aatcggagct tttcatcgct
     1321 ctctcctaaa catcgaacac caccgtaacc tccagcccgg cctccgtttg ccggatcgcc
     1381 agatcatggt aggtgacggc tttgatatgc ttctgaatct cggccggccg tccgcccgac
     1441 actgtgccga ccagtcgggt cggcgtcaca tcacgcaggt cggccaccgg aaaaaccagc
     1501 ccgtcgcgct cggcccaata gaccaactca cccaggaaaa gcacgagcat ctcctccgca
     1561 tcataggcct ccagagtcag atcgcgcgat aggtctcgcg gcacgaccga cgtatcaccg
     1621 gccagcaggt acgccatccc ctcggccgcg cgagcgaata aagctttcag gtcatcaccg
     1681 cgcaggcgca aagcccagtc ggccgtgtgc tcgacgatgc gaatgtcatc gtcctcaggt
     1741 gcatccatca tcttgctatt ataccggtac tcagtattcc agaagttgaa agctgtccag
     1801 aatgacgttc agatcacgct gcaaggctcc gaaatgttcc tgcggagcgc aggcggccac
     1861 caggtatccg ggcttgcctt caccgctgac gaacaggtgg cggcattgat actcattgga
     1921 cggatccagc gcgatgaaac gcgcctgtgg ctgaccggca aggtgcgtat tgatctcctt
     1981 ttcggatatc tgaaaagagt tctcgtccag cgcatccagc acgtcctgcg gggccacgcc
     2041 gcgcagacgc tcgctccggc cgatgagcag gaacggttgc ggatcgactt cgtccagcaa
     2101 gccggcacct acggccaggc ccaccgtctc cacatcatca gcaagctcgc ccagcggccc
     2161 taaggcgcga ttgacatagg tttgctgcgc cacaacgcgc gacagcaact cgctctgatc
     2221 ccgataggcc atgtcgatcc attgccattc gatagggata gacaccgtat agcgcagatc
     2281 ggcatcggtc accctgaccc attcgccatc cgaatcgaaa ccggcgccgc tcacgaggac
     2341 gggccgcagg ctcaaagagc gcatgaccag ccagaggccg gcgatgatcg ccagcgtcag
     2401 cagcccatag accagcagcg cacgccagtt atagtcagcc ggcgtgacat cctcttcccg
     2461 cgcagccaga tccggcgttt ttgtgaatgc gcgcttgggg gttggggcgg ccgctctctt
     2521 cggcagctga tagccacaat gccagcatac gacatcagtc gctaagatcg gcttgtcgca
     2581 attggggcat cgatcggcca aaataaactc tctccatcgc accgtcgcca aagtgtaaag
     2641 cgaattacac ctctcttgta aaatccttga acgacaacgc agattacaag cgctatcata
     2701 acgtcatcat tcagcaacac aaatagtcct cc
//
LOCUS       DAOMQG010000425         2729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14975_length_2729_cov_8.669783, whole genome shotgun sequence.
ACCESSION   DAOMQG010000425 DAOMQG010000000
VERSION     DAOMQG010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14975_length_2729_cov_8.669783"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..2438)
                     /locus_tag="PLC06_15460"
     CDS             complement(9..2438)
                     /locus_tag="PLC06_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRO91896.1"
                     /translation="MSFRKWMMTLLVVAVLPVLVACGGDAEPATTDATVAPTIVAQPT
                     ADLPVDAAGDLLVVATDAPLPPFADFNPFGEVVGFNNALMERIAGRAGMDYEFVVTPS
                     EGVLDTIATGSAHDFDAVMSALLIPETPPQGIAFTHPYLEVGQVLMVLVDEKAINSAA
                     DILPGIAVGVMTNSAGQDAAAALGIADSDLFATYEKPSQLVQALIDEVVEVIIIDSYA
                     AEYFVNNFPEQVRVAGGEGREAWLDYRAYGIAVAADDTQLLDTLNSAIDDLRDDGELN
                     QLTVDWLISEADPAAAVDPGESRVGTSANELYIGLVGQFTDMDPASLTTDFIGWEIKN
                     NTMSGLYRFTPDNVLEPLLASALPAVSEDKLEYTIPLRTGLRFPDGSEFTAEDVKWSL
                     NRAAGLGNFLVNSYLKDSNGDNFADEDAVQVMDPATVKIILKEPTGYFPAILTAPPFF
                     PVSSECYSEAGDPGSTCGGLGPYTIVSWELNDRMRLRANPDWPGQPAPAFENLTLRFY
                     DDNVSLRRSLVEFRSVDLAWTGLSFQDYTELTAVDLDADGAGDYAGWEGPFTFKSYII
                     FEQSTKPWDSERVRQAAAYAIDHEALAAVFGGARQGLRSPVPSTVPGHVPTLPARDLE
                     RARELLRVEGYTADKKLPITLWYVNDGRYSANEEAYANAIKAQLEETGVFQVELAGAG
                     WDEFRLQISQCGFPAYLLGWPSPGQPTSYLDATSWTDFFVTNTNRVFCSNYESDKMTK
                     LVAEARTEIEEGPRLELYGRIQTLWAEELPTLPLTEESRRAVSLPTVGGVRINGLGMM
                     HYEYLTKEVGQ"
BASE COUNT          531 a          909 c          806 g          483 t
ORIGIN      
        1 ccactgacct actggcccac ttccttcgtt aaatactcgt aatgcatcat ccccagccca
       61 ttgatgcgaa cgccaccgac ggtgggcagc gacacggcgc ggcgcgactc ctcggtcagc
      121 ggcagcgtcg gcagctcctc ggcccatagc gtctggatgc ggccgtacag ctccaggcgc
      181 ggcccttcct caatttcggt ccgcgcctcg gccaccagtt tggtcatctt gtcgctctcg
      241 taattggaac agaacacccg attggtgttg gtcacgaaga aatcagtcca gctcgtggcg
      301 tccagatagc tggtcggctg gccgggcgaa ggccaaccca gcagataggc cgggaaaccg
      361 cactgcgaga tctgcaagcg gaactcatcc caacccgccc cggccaactc cacctgaaac
      421 acccccgttt cctctagctg cgccttgatc gcgttggcat aagcctcctc gttggcgcta
      481 tagcggccgt cgttgacata ccacaatgtt atcggcagct tcttgtcggc ggtatacccc
      541 tcaacccgca ataattcgcg ggcgcgctcc aggtctcgcg ccggcagcgt cggcacatgg
      601 ccgggcacag tgctgggtac ggggctgcgc aacccctgtc gcgccccgcc aaacacggcc
      661 gccagcgcct cgtggtcaat ggcataggcc gccgcctgcc gcacgcgctc gctgtcccac
      721 ggcttcgtcg attgctcaaa gatgatgtag ctcttgaacg tgaacggccc ttcccagccg
      781 gcataatccc ctgcgccgtc ggcgtccaga tcgacggccg tcaactcagt gtaatcctgg
      841 aagctcagcc ccgtccaggc cagatcaacc gagcggaact ccaccaatga tcggcgcagc
      901 gacacgttat catcgtaaaa ccggagcgtg agattctcga aagccggcgc gggctgcccc
      961 ggccaatcgg gattggcccg cagccgcatc cggtcattca gctcccaact gacgatggta
     1021 taggggccaa gcccgccaca ggtgctgccg gggtcgcccg cctcggaata acactcgctg
     1081 ctgacgggga aaaacggcgg cgcggtcaga atcgcgggga aatagcccgt cggttccttc
     1141 aggataatct tcaccgtggc cgggtccatc acctgcacgg cgtcctcatc ggcgaaattg
     1201 tcgccgttgc tgtctttcag atagctgttc accagaaagt tgcccagacc ggcggcgcgg
     1261 ttgagcgacc acttaacgtc ctcagcagtg aattcgctgc cgtcggggaa gcgcaacccc
     1321 gtgcgcaggg gaatcgtgta ctccagcttg tcctcggaga cggcgggcaa agccgaggcc
     1381 agcagcggct ccagcacatt atcgggcgtg aagcgataga ggccgctcat ggtgttgttc
     1441 ttgatctccc agccgatgaa atcagtcgtc aacgaggcgg gatccatgtc cgtaaactgg
     1501 ccgaccagac cgatgtacag ctcattggcc gaggtgccga cgcgcgactc gcccggatcc
     1561 acggccgccg ccgggtcggc ctcggagatc agccaatcga cggtcagctg attcagctcg
     1621 ccgtcatcgc gcaggtcgtc gatggcgctg ttgagcgtat ccagcagctg ggtgtcgtcg
     1681 gcggccacgg cgatgccata ggcgcgatag tccagccacg cctcgcgccc ctcaccgccc
     1741 gccacgcgca cctgctccgg gaagttattg acgaaatact cggccgcata gctgtcgatg
     1801 atgatcactt cgaccacctc atcaatgagc gcctgcacca gttgcgacgg cttctcatag
     1861 gtcgcgaaga gatcgctatc ggcgatgccc aacgccgcgg ccgcgtcctg ccccgcgcta
     1921 ttggtcatca cacccacggc gatgccgggc aaaatatcgg ccgcgctgtt gatcgctttc
     1981 tcatccacca gaaccatcag cacctggccc acctcaagat agggatgggt gaaggcgatc
     2041 ccctggggcg gcgtttcagg gatgagcagg gccgacatga cggcgtcgaa gtcatgggca
     2101 gagccggtgg cgatggtgtc cagcacaccc tcgctggggg tgacgacgaa ctcgtaatcc
     2161 atccccgccc ggccggcgat ccgctccatg agcgcgttat tgaagcccac cacctccccg
     2221 aacgggttga agtcggcgaa gggcggcagc ggcgcgtcgg tggcgacgac cagcaaatcg
     2281 ccggcagcgt ctaccggcag gtcggccgtc ggctgcgcga caatcgtcgg cgcaacggtc
     2341 gcgtcggtgg tcgcgggttc cgcgtcgccg ccacaagcca ccagaaccgg cagcaccgcc
     2401 accaccagca gggtcatcat ccatttacga aacgacattg aaatcctctt tgagcggccg
     2461 aattcaggcc gctcgccaag tataacgagg gagaacaagc gggcaagcgc ggcccggaca
     2521 gctctaagaa tgtggaatag ctggagaatt tgtgcgaatc ggaaccggtg cgccttcgtg
     2581 ctcccccatg acgaaggcgc gttcacgttg aactctcttt gtccggcccc atggccactc
     2641 aacggacttc ccaatcacgc aagcggccgt cccgcatcgg tgaattgtga cgccagatgg
     2701 atttgaacca tcgacctagg gcttatgag
//
LOCUS       DAOMQG010000426         2727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_14984_length_2727_cov_8.278817, whole genome shotgun sequence.
ACCESSION   DAOMQG010000426 DAOMQG010000000
VERSION     DAOMQG010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_2727_cov_8.278817"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2522
                     /locus_tag="PLC06_15465"
     CDS             <1..2522
                     /locus_tag="PLC06_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRO91897.1"
                     /translation="RDFVANVVAWERIPARSGRYASMPDSVDPRLVAALRARGIEQLY
                     SHQAAAVDAVARGENVVVATTTASGKSLTYTLPVLSRLAADPAARALYLFPTKALAHD
                     QEAETAAIINAARLPIPVHTYDGDTPRGKRSSIRAGGGVLITNPDMLHAGILPFHPSW
                     RSLFGRLEYVVVDEIHTYRGVFGSHVANVLRRLQRLCRFYGSDPQFICASATIANPRE
                     HAERLIEQPFTLIDESQNGAPQGRKDFILYNPPIIDEELGLRAGTVPAAKDAAATFLA
                     QDIQTIVFARARQTVELLLSYLRDELAYLGRDVTPLDGQLPAVMGYRGGYLPLERRAI
                     EAGLRDGTVRGVVTTNALELGIDIGALDAAVLTGYPGTIAGTWQQVGRAGRRAERSAA
                     ILVAGNGALDQYLCLHPRYLFGRSPEHALTNPDNLPILTRHLLCAAFELPFADDEAFG
                     GFDPVKPVLDDLARAGDLHATRNQYHYVGDGAPSSGVSLRTGGDDTVVIQDLSGDQPN
                     VIGQIDLAGAAQMVHEGAIYMHQARTYLVDRLDWDGRVADVYPVDVDYYTRANIGSTI
                     RRLEAVDEQERDGLTIAHGEATVVTQATGYRKVRRYTNETLGFGPIDLPAMTLETTGY
                     WLVFSEALTDKLYDLGILLRPNDYGPNWQAQRQLALVRDGHRCRTCGASGDDIVLHVH
                     HIRPFREYGYVPGENENYKAANQIDNLVTLCPGCHRRAEAGQQTRSALAGLGYTLGNL
                     APLFLMCDPSDLSVSAEALSPGTRAPTLAIYERVPAGVGFSARLYELHGTLLAAALEL
                     VSDCQCRNGCPACVGPPGDIGPDTKDVTRRLLGILSGNQPL"
BASE COUNT          458 a          971 c          873 g          425 t
ORIGIN      
        1 accgcgactt cgtcgccaat gtcgtcgcct gggagcgcat cccggcccgg tccggccgct
       61 atgcgtccat gcccgactcg gtcgatccgc ggctggtcgc cgccctgcgg gcgcgcggca
      121 tcgagcagct ctacagccac caggcggcgg ccgtcgacgc cgtggcccgc ggcgagaacg
      181 tcgtcgtcgc cacgaccacg gccagcggca aatcgctgac ctacacgctg cccgtcctct
      241 ctcgcctggc ggccgacccc gcggcccgcg ccctttacct cttccccacc aaggcgctgg
      301 cccacgacca ggaagcggag acggcggcca tcatcaacgc cgcccggctg cccatcccgg
      361 tccacaccta cgacggcgac accccgcgcg gcaagcggtc gagcatccgc gccggcggcg
      421 gcgtgcttat caccaatccc gacatgctcc acgccgggat tctgcccttc cacccgtcgt
      481 ggcgctcgct cttcgggcgg ctggagtacg tcgtcgtcga cgagatccac acctaccgcg
      541 gcgtcttcgg cagccacgtg gccaacgtcc tgcgccgcct gcagcgcctg tgccgcttct
      601 acggcagcga tccgcagttc atctgcgcct cggccaccat cgccaacccc agggagcacg
      661 ccgaacgatt aatcgaacaa ccgttcaccc tgatcgacga atcccaaaac ggtgcaccgc
      721 aaggccgcaa ggacttcatc ctctataacc cgccgatcat cgacgaggaa ctgggcttgc
      781 gagccggcac cgtgccggca gccaaggacg cggccgccac tttcctggct caggacatac
      841 aaaccatcgt cttcgcccgc gcccggcaga cggtggaact gctgctcagc tatctgcgcg
      901 acgaactggc ttatctgggc cgcgacgtga cgccgctcga cggtcagttg ccggccgtga
      961 tgggctaccg cggcggctat ctgccgctgg agcggcgcgc catcgaggcc gggctgcgcg
     1021 acggcacggt gcgcggtgtc gtgaccacca acgcgctgga actgggcatc gatatcggcg
     1081 cgctcgacgc ggccgtgctg accggctacc ccggcacgat cgccggcacg tggcagcagg
     1141 tgggccgggc cggacggcgg gccgagcgct cggcggctat tctcgtggcc gggaacgggg
     1201 cgctcgacca gtacctctgc ctgcacccgc gctatctgtt cggccgctcg ccggagcacg
     1261 cgctgaccaa ccccgacaac ctgcccatcc tgacccgcca cctgctctgc gccgcctttg
     1321 agctgccctt cgccgacgac gaggccttcg gcggcttcga cccggtgaag ccggtgctcg
     1381 acgatctggc gcgggcgggc gacctgcacg ccacgcgcaa ccagtaccac tacgtcggcg
     1441 acggcgcccc ctcgtcgggc gtgtcgctgc gcaccggcgg cgacgacacg gtcgtgatcc
     1501 aggatctcag cggcgaccaa ccgaacgtca tcggccagat cgatctggcc ggggcggcgc
     1561 agatggtcca tgagggggcg atctacatgc accaggcgcg aacctatttg gttgaccgcc
     1621 tcgattggga cggccgcgtg gccgacgtgt atccggtgga tgtcgattac tacacccgcg
     1681 ccaacatcgg cagcaccatc cggcggctgg aggcggtgga tgagcaggag cgggacggcc
     1741 tgaccatcgc ccacggcgag gcgacggtcg tcacccaggc caccggctac cgcaaggtgc
     1801 gccgctatac caatgagacg ctcggcttcg gccccatcga tctgccggcc atgacgctgg
     1861 agacgaccgg ctactggctg gtgttcagcg aggccctgac cgacaaactc tatgacctgg
     1921 gtatcttgct gcggccgaac gactacggcc ccaactggca agcgcagcgc caactggcgc
     1981 tggtgcgcga cggccaccgt tgccggacgt gcggcgcgag cggcgacgat atcgtgctcc
     2041 atgtccatca catccggccg ttccgcgaat acggctatgt gcccggcgaa aacgagaact
     2101 acaaggccgc caaccagatc gataacctgg tgacgctctg ccccggttgc caccggcggg
     2161 ccgaggccgg gcagcaaacg cgctcggcgc tggccggtct cggctacacg ctgggcaatt
     2221 tggccccgct gttcctgatg tgcgatccga gcgacctcag cgtctcggcc gaagcactca
     2281 gccccggcac gcgcgcgccg acgctggcca tctatgagcg cgtgccggcc ggggtgggtt
     2341 tcagcgcccg gctctatgaa ctccacggca cgctgctggc ggccgcgctg gaactggtca
     2401 gcgattgcca atgccgcaac ggctgcccgg cctgcgtcgg cccgccgggc gacatcgggc
     2461 cggatacgaa ggacgtgacg cggcggttat tggggatatt gagcgggaac caacctttat
     2521 aaacgggtat tgtgtataca catgtgtaga tgtatactgc tcttgaggta aatacaatga
     2581 ccgatcttta tcgcgcacaa gtcctgttgg aacgcaagca gcatcaggcg ctgcaagctc
     2641 tggccgcggc cgaggggcgc agcatgtcgg agatcatccg tgaggctgtg gctgagtatc
     2701 tggtcgaaca gactgataaa gacgagt
//
LOCUS       DAOMQG010000427         2718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15043_length_2718_cov_4.867067, whole genome shotgun sequence.
ACCESSION   DAOMQG010000427 DAOMQG010000000
VERSION     DAOMQG010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15043_length_2718_cov_4.867067"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..694)
                     /locus_tag="PLC06_15470"
     CDS             complement(98..694)
                     /locus_tag="PLC06_15470"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HRO91898.1"
                     /translation="MDEILPEQRRAQLLEWLNERGSLTIDEMARQFGVSSMTIHRDID
                     RLEKDGLARKVRGGVMPVADAPDQTPPAQSRCAMCGKVVARRTAWVATTDQGEQWQAC
                     CAHCGLLRLHHTAGAQSVLAADFLFGQMVNVYQATFVLGSSVTLCCVPSVLCFATRHD
                     AERYARGFGGELLDFNGALAAMGDAHHGHDAGDRSVRR"
     gene            957..1355
                     /locus_tag="PLC06_15475"
     CDS             957..1355
                     /locus_tag="PLC06_15475"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRO91899.1"
                     /translation="MSLPMPNGAVYMTITNGTADDDALIDAAVPGCDAVELHEMIMEN
                     DVMVMRQMEGNRIPIPAGETVVLQRGGLHVMCIGKTAEFAVGESVPVTLTFEKAGTME
                     VTAEVIDPGETAPEMQMEKEEGGMDMEQND"
     gene            1381..1872
                     /locus_tag="PLC06_15480"
     CDS             1381..1872
                     /locus_tag="PLC06_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRO91900.1"
                     /translation="MSRRIVKGSIALLIVLSVVGAAAGCRRAPVSEPGATMPGRPVSS
                     GEVSSDEPSPDGIRISLVAPLFPPPAGDGHLAFRVTDAGEQPIDTATLQIRGDMTHAG
                     MLPIEATATADEDGVYRTPIRWTMAGDWIITVEATLSDGRRATQSFNVTVTGEEESCI
                     DDL"
     gene            1854..2471
                     /locus_tag="PLC06_15485"
     CDS             1854..2471
                     /locus_tag="PLC06_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRO91901.1"
                     /translation="MHRRFIRGWRGAAFFLVALFIIGLSACAVEQTHEFAGAVLESPE
                     PLPEFTLMSAEGPVSLSDFRGQYVFLYFGYMFCPDFCPTTMAKLAKVHRDLGDDAERM
                     QVIMISVDPERDTPDALAKYAAAFNPTFLGITGTKEEIDLVGEPYGLYYQRNEGSAAT
                     GYLVDHSTRTYLIGPDGRALVAYPHDASAEGIAADLRWLLEQESR"
     gene            complement(2538..>2718)
                     /locus_tag="PLC06_15490"
     CDS             complement(2538..>2718)
                     /locus_tag="PLC06_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO91902.1"
                     /translation="GEHRGRGYGKALVAAVLAHPELQGLRRWLLATKDAHGLYAQNGF
                     TPVPDGRFMERLISR"
BASE COUNT          527 a          847 c          843 g          501 t
ORIGIN      
        1 tcggcatggg caagacgccg gagccgtggt actccatccc ctggcgctgg tgggaggcgg
       61 gcaatccgtt cgtgtcgaaa ggctccggca agcgcggtta gcggcggacg gagcggtcgc
      121 ctgcgtcgtg gccgtggtgg gcgtctccca tagcggccag cgctccattg aaatccagta
      181 actccccccc gaacccccgt gcgtagcgct ccgcgtcgtg gcgcgtggcg aagcacaaca
      241 cgctgggcac gcagcagagc gtcacgctgg aacccagcac gaacgtcgcc tggtagacgt
      301 tgaccatctg gccgaagaga aaatcggctg ccagcaccga ttgcgcgccg gcggtgtgat
      361 gcagacgcaa caacccgcaa tgggcgcaac acgcttgcca ctgctccccc tgatccgtag
      421 tggcgaccca tgccgtgcgt cgcgccacga ccttgccgca catcgcgcat ctgctctggg
      481 caggcggcgt ctggtcgggc gcatcggcaa ccggcatgac cccgccccgt accttgcggg
      541 ccaggccgtc cttttccagt cgatcgatgt cacggtggat ggtcatggac gacacgccga
      601 actgccgggc catctcatcg atcgtcagcg agccgcgctc attgagccat tccaggagtt
      661 gcgcgcgccg ttgttccgga agtatttcgt ccattatctc ctcgttatag atcatgattc
      721 ctctactatc ctaacagaaa aactcccctc ttgtcagccg ttcgctgacg ttttataatg
      781 tgctgtaatg tgatataaca cataatataa cataatatca cacacaaggg agagctatga
      841 agcgattggt tgcacttggc ctgataatgg ttgtgtcaat gatgggctgc ggcgggacag
      901 aaatggggga aaccgatgat ggagggcacc tcatcgtcgg aaatgttttg gccaatttgt
      961 ctttaccaat gcccaatgga gcggtctaca tgaccatcac caacggcacc gccgacgatg
     1021 acgccttaat cgacgcggct gtgcccggct gcgacgccgt cgaactgcat gagatgatca
     1081 tggaaaacga cgtcatggtg atgcgccaga tggaaggcaa tcgcatcccg attcccgccg
     1141 gcgaaacggt cgtgctccag cgcggcggcc tgcacgtcat gtgcatcggc aagacggccg
     1201 agtttgccgt gggcgaatcc gtgcccgtca cgctgacgtt cgagaaggcc ggcacgatgg
     1261 aagtgacggc cgaggtcatc gatccgggcg aaacggcacc ggagatgcaa atggagaagg
     1321 aagaaggcgg catggacatg gagcaaaacg actagctcgc cgatggatga attgtttccc
     1381 atgtcgcgac gaatagttaa ggggagtatc gcgcttctca tcgtgctatc ggttgtgggc
     1441 gcggcggccg ggtgccgtcg cgcccctgtc tccgagccgg gcgcaacaat gcccggtaga
     1501 cctgtctcat ccggcgaggt atcgtccgac gagccgtcac ccgacggcat ccgcatctcg
     1561 ctcgtcgctc cgctgttccc gccgcccgcg ggcgacggcc acctggcttt ccgcgtcacg
     1621 gatgccggcg agcagcccat cgacacggct acgctgcaaa ttcggggtga tatgacccat
     1681 gccgggatgc tgccgattga ggcgacggcc acggcggacg aggacggcgt ctatcggacg
     1741 cccattcgct ggacgatggc cggcgactgg atcatcaccg tcgaggcgac actgagcgac
     1801 ggccgccggg cgacgcaatc cttcaatgtg acggtgacgg gagaagaaga atcatgcatc
     1861 gacgatttat aagaggatgg cgcggagccg cgtttttcct cgtcgccctg ttcattatcg
     1921 ggttgagcgc gtgcgcggtt gagcagaccc acgagttcgc cggcgcggtg ctggaaagcc
     1981 cggagccgct gccggaattc acgctgatgt cggccgaggg gccggtcagc ctgtccgact
     2041 ttcgcggcca atacgtcttc ctctatttcg gctacatgtt ctgccctgat ttttgcccca
     2101 ccaccatggc caaactggcg aaagtccacc gcgatctggg tgacgacgcc gagcgaatgc
     2161 aagtcatcat gatctcggtc gatcccgaac gggacacgcc cgacgcgctg gccaaatacg
     2221 ccgcggcatt taacccgacc tttttgggca tcaccggcac gaaagaggaa atcgatcttg
     2281 tcggcgagcc atacggcctc tattaccagc gcaacgaggg cagcgccgcc accggttacc
     2341 tcgtcgacca ctccacccgc acctacctaa tcggccccga cggccgcgcg ctggtggcct
     2401 atccccatga cgcttcggcc gagggcattg ccgccgatct gcggtggctg ctggagcagg
     2461 aatcccggtg atataaagaa gttgagccga aatagttgag acggcggcac agcgcgggat
     2521 ggattacaaa tctgcctcta ccggctgatg agccgttcca tgaatcgccc gtccggcacg
     2581 ggggtgaagc cgttctgtgc gtagaggccg tgggcgtcct tcgtcgccag cagccagcgc
     2641 cgcaagccct gcagctccgg gtgggccagc acggccgcca ccagcgcctt gccgtagccg
     2701 cggccgcgat gttcgccg
//
LOCUS       DAOMQG010000428         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15048_length_2717_cov_8.010894, whole genome shotgun sequence.
ACCESSION   DAOMQG010000428 DAOMQG010000000
VERSION     DAOMQG010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15048_length_2717_cov_8.010894"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLC06_15495"
     CDS             complement(<1..282)
                     /locus_tag="PLC06_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91903.1"
                     /translation="MDTLVWIALIVAAAVIIILYMFRGARSKPPLKADRDRLEAGTET
                     WEPTTAVIVLENAGQVEQLKILGKGTVGDIAFSPDSRTLAVGGSIGIHLY"
     gene            complement(428..760)
                     /locus_tag="PLC06_15500"
     CDS             complement(428..760)
                     /locus_tag="PLC06_15500"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRO91904.1"
                     /translation="MAGIRFYLDENLPVAVAEQLRRRGIDAVTVRDLGLLGQSDIDHL
                     ALARSSGRVLRTHDADYLELAQEGEAHGGIIFGQQQIHGVGSWVNFLELAYNVYSAEE
                     MIGRVEYL"
     gene            complement(732..1034)
                     /locus_tag="PLC06_15505"
     CDS             complement(732..1034)
                     /locus_tag="PLC06_15505"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRO91905.1"
                     /translation="MQTIQSINQIATDSHVRNGRPYIVGTSVTVADVAIVKIYHSQDA
                     DGIAQWYGLDLSQVYAALAYYYEHKEAMDADIRAQIRRAEALRENRAGGRDSLLPR"
     gene            complement(1080..2153)
                     /gene="mtnA"
                     /locus_tag="PLC06_15510"
     CDS             complement(1080..2153)
                     /gene="mtnA"
                     /locus_tag="PLC06_15510"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRO91906.1"
                     /translation="MRTVFWDYDRDVMKMIDQRLLPFELVVPEYTTWQDVAAGIRDMV
                     VRGAPAIGAAAGFGMALAARQSTAADRPALLRDLEAAGQYLEAARPTAVNLAWGVRRL
                     LRVATSEELETAADVRDALLVEAQRLADEDVALNRRMGFNGAELISDGDTILHHCNTG
                     ALATVDWGTALGVVFAAHEQGKRVHVLVDETRPRLQGARLTAWELKERGISFDLIADN
                     AAGHFMRAGEVDIVLVGSDRTAANGDVCNKIGTYQVAVLAQANGVPFYPVVPTSTIDL
                     NLATGDLIPIEERDDDEVLVIGSQRIAPEGITARNPAFDVTPHRYVTGIVTEAGIVYP
                     PFVKNLRAAVATAAVENERAGGR"
     gene            2449..>2717
                     /locus_tag="PLC06_15515"
     CDS             2449..>2717
                     /locus_tag="PLC06_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91907.1"
                     /translation="MNNKLLGLLMIPGLLVFGLAAARGDAIGEPRLGEPPQAPARALV
                     EEPREAETGSAAYTLFLPSIQAPPPWVNTASRAESQQFFRTGYLGT"
BASE COUNT          486 a          893 c          825 g          513 t
ORIGIN      
        1 gtacagatgg atgccgatgg agccgcccac ggcgagcgtc cggctatcgg gagagaaggc
       61 aatatcgcca accgtgccct tgcccagtat tttcaattgc tcgacctggc cggcgttctc
      121 gagaacgata acggccgttg tgggttccca cgtttcggtc ccggcttcca gacggtcgcg
      181 gtcagcctta aggggaggtt tgctgcgagc cccgcgaaac atgtagagaa tgataatcac
      241 cgctgccgcg acgatcagcg cgatccaaac gagggtatcc atcatagcct cctgttcaag
      301 ctgaactgaa acctgagagg ataacctcaa gcttccagcc gttcaaatcc cgaaaatcat
      361 gattatcccg cccgtctttt ttcaccacgg ccgacaaaac aacgggtcag caatcccatt
      421 cctcccgcta taaatattcg acccgcccga tcatctcttc cgcactgtaa acattgtagg
      481 cgagttcgag gaaattgacc cagctgccga cgccgtggat ctgttgctgc ccaaagataa
      541 tgccgccgtg cgcctccccc tcctgcgcca gttccagata gtcggcatca tgggtgcgaa
      601 ggacacggcc gctgctgcgg gccagagcca ggtggtcgat gtcgctttgt cccagcaagc
      661 ctaaatcacg gacggtgacg gcgtcgatcc ctcgtcgccg caactgttcg gcgacggcca
      721 ccggcaggtt ctcatcgagg tagaagcgaa tcccggccac cggcgcggtt ctccctcaac
      781 gcctctgcgc gcctgatttg cgcgcgaata tcggcatcca tggcttcctt atgttcataa
      841 tagtaagcca gcgccgcata cacttgcgac aaatccagcc cgtaccattg cgcgatgccg
      901 tcggcgtcct ggctgtggta aatcttcacg atggcgacat cggcgacggt gaccgacgta
      961 cccacgatat aagggcggcc gtttcgtacg tggctgtccg tggcgatttg attgatgctc
     1021 tgtatcgttt gcatcgtgca cctcattggg tcgtggggaa attataccac ttgctccgcc
     1081 tatcgcccgc ccgcgcgctc attctccacc gccgcggtgg ccaccgccgc ccgcaaattc
     1141 ttcacaaacg gcgggtagac gatccccgcc tcggtgacga tgccggtcac gtagcggtgg
     1201 ggggtcacgt cgaaggccgg gttgcgggcg gtgatgccct ccggggcgat gcgctggctg
     1261 ccgatgacca gcacctcgtc gtcgtcgcgc tcctcgatgg ggatcaggtc gcccgtcgcc
     1321 aggttaaggt cgatggtgct ggtgggcacg acggggtaga agggcacgcc gttggcctgc
     1381 gccagcaccg ccacctgata ggtgccgatc ttgttgcaca cgtcgccgtt ggccgccgtg
     1441 cggtcgctgc ccacgaggac gatgtccacc tcgccggcgc gcatgaagtg gccggcggcg
     1501 ttgtcggcga tgaggtcgaa cgaaatgccg cgctccttca gctcccaggc cgtcaggcgc
     1561 gccccctgca gccgcggccg cgtctcgtcc accagcacgt ggacgcgctt gccctgctcg
     1621 tgggcggcaa agaccacgcc cagcgccgtg ccccaatcga ccgtggccag cgcgccggtg
     1681 ttgcagtggt ggaggatggt gtcgccgtcg ctgatcagct ccgcgccgtt gaagcccatg
     1741 cgccggttga gggccacgtc ctcgtcggcc agccgctggg cctcgaccag cagcgcgtcg
     1801 cgcacgtcgg cggccgtctc cagctcctcg ctcgtcgcca cgcgcagcag ccgccgcacg
     1861 ccccaggcca gattgacggc cgtcggccgc gccgcttcca gatattgccc ggccgcctcc
     1921 agatcgcgca gcagcgccgg gcggtcggcc gccgtgctct gccgggcggc cagggccatg
     1981 ccgaagcccg ccgccgcgcc gatggccggc gcgccgcgca cgaccatatc gcggatgccc
     2041 gcggccacgt cctgccaggt ggtatattcc ggcacgacca gctcgaacgg cagcagccgc
     2101 tggtcgatca tcttcatcac gtcgcggtca tagtcccaaa aaacagtgcg catgaatccc
     2161 tccggagaat gaattacgaa ttacgaatta cgaattacga atgaagaggt tcattcgtgg
     2221 tcatgtagcg cgacctgtca ggctgcgtcc acgaccctaa ttctattgga tggtggcggg
     2281 tcggccaaac gcgggcgaat atgagaaacc ctgaatgatc ggccgcaaat tcctcgccaa
     2341 tgcgggtaaa accgccggcc gtctattata atgctcagcg gtttgctgga ttgccatggt
     2401 cggccgttcg ttcatccatt tgacaataca attcgggggg tgaaaaccat gaacaacaaa
     2461 ctccttggct tgctgatgat tccgggcttg ttggtgtttg gcctggccgc ggcaagggga
     2521 gacgccatcg gcgagccgcg cctgggagag cctccgcaag ccccggcgcg cgccctcgtg
     2581 gaagagccac gcgaggcgga gaccggaagc gcggcctaca ccctgttcct gccgtccatt
     2641 caggccccgc cgccatgggt gaatacggcg tctcgcgcgg agtcgcagca gtttttccgg
     2701 accggctatc tgggcac
//
LOCUS       DAOMQG010000429         2710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15096_length_2710_cov_8.854614, whole genome shotgun sequence.
ACCESSION   DAOMQG010000429 DAOMQG010000000
VERSION     DAOMQG010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15096_length_2710_cov_8.854614"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLC06_15520"
     CDS             complement(<1..526)
                     /locus_tag="PLC06_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91908.1"
                     /translation="MTVPRDTHEADRTGMVRVGRILSNIISPPIMFAAIGFALGVYER
                     PIWPGLAWGFVYGSLVAVTPLLVIAWLMHIGRIRELHMSNTHERHIPYLTSVISSAVA
                     FLILTLFNGPELLRCLSVLSAITLGVLGLINTKWLISIHATAAGATWMIATVVFGWVA
                     GAILLPFVILISAIR"
     gene            complement(632..1894)
                     /gene="ahcY"
                     /locus_tag="PLC06_15525"
     CDS             complement(632..1894)
                     /gene="ahcY"
                     /locus_tag="PLC06_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRO91909.1"
                     /translation="MDFDVKNIDLAPGGRHRIDWAEQEMPVLRGVLKQFKNDRPFAGL
                     RISACMHVTTETANLMVSLQAGGADVVLSASNPLSTQDDVAAALVSHYEIPVYAIKGE
                     DNETYFKHINAVLDHQPHIVMDDGADLVSTIHKSRRNLIDNMLGGTEETTTGIIRLRA
                     MARDGALQFPMMAVNDALTKHMFDNRYGTGQSTMDGIIRATNVLLAGKNVVVAGYGWC
                     SRGIAMRARGLGANVIVTEIDPLKALEAVMDGYRVMPMNDAAPIGDIFVSATGDIHVF
                     DEHHFMVMKDGALMCNSGHFDVEINKPALRELATGEPKRVRPFVEQFVMKHNGHRLNL
                     LGEGRLINLAAAEGHPSAVMDMSFAGQAMAAKYLVDHKGELENRVYTVPQEVDQDIAR
                     IKLDAMGIKIDTLTEMQASYLGSWEEGT"
     gene            complement(1932..>2710)
                     /locus_tag="PLC06_15530"
     CDS             complement(1932..>2710)
                     /locus_tag="PLC06_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRO91910.1"
                     /translation="FGEYRAFLDSHGIDTSAILDIEDEFCASFFVNTDEDNNQIASFY
                     TGAMAYAGKLSFARYAPDAELTIITPNDPGAMRSYIRECKSIGLPFIYDPSQQTARIS
                     AEEILEGIDGCYLLSSNEYEFQLIQEKTGLSEAEILDRAGGLVMTMGPSGSRLVVDGR
                     EEFVPVAPTRWVAEPTGAGDAYRAGLMRGMQLGLPWGVAGRMGALAAAYVLEQSGPQN
                     HYFTREEFVARYRESFDDEGALDVLLQEPETIKASAPVVH"
BASE COUNT          525 a          832 c          826 g          527 t
ORIGIN      
        1 ggcggatggc cgagatcagg atcacgaacg gcagaagaat ggcgccggcc acccagccaa
       61 agacgaccgt ggcgatcatc caggtcgcgc cggcggccgt ggcatggata ctgatcagcc
      121 atttggtgtt gatcagcccc agcactccaa gggtaatggc gctgaggacg gacaggcagc
      181 gcagcaactc ggggccattg aacaaggtca ggatgaggaa ggccaccgcg ctggaaatga
      241 ccgaggtcag ataggggatg tggcgctcat gggtgttgct catgtgcagc tcgcgaatgc
      301 ggccgatgtg catgagccag gcaatgacga ggagaggcgt cacggcgacc agcgagccat
      361 agacaaaccc ccaggccaac ccgggccaga tggggcgctc ataaacgccc agggcaaacc
      421 cgatcgccgc gaacatgatc ggcgggctaa tgatgttgga cagaatccgc ccaacccgca
      481 ccattcccgt gcggtctgct tcgtgtgtat ctcttggtac ggtcatggcg gcccggcgac
      541 cgaaccggtc gccccttccc acaaaatggc cggcgcgatg ggtcaagagg atccccaccg
      601 cgccggccgg tattcagtca aacaatacgg atcaggtacc ctcttcccac gagccgaggt
      661 aggaggcctg catctcggtc agggtgtcga tcttgatccc cattgcgtcg agcttgatgc
      721 gcgcgatatc ctggtcgact tcctgcggga cggtgtagac gcggttctcc agctcgccct
      781 tgtggtcgac cagatacttg gcggccatcg cctgcccggc aaagctcatg tccattacgg
      841 ccgacgggtg gccctcggcc gcggccaggt taatcaggcg gccctcaccc aacagattca
      901 gccgatggcc gttgtgcttc atcacgaact gctcgacgaa cggccgcacg cgcttcggct
      961 caccggtcgc cagctcgcgc agcgccggtt tgttgatctc cacatcgaag tggccggaat
     1021 tgcacatcag cgccccgtcc ttcatgacca tgaaatggtg ctcgtcgaag acgtggatat
     1081 cgcccgtggc cgacacgaag atgtcgccga tgggagcagc gtcgttcatc ggcatgacgc
     1141 gataaccgtc catcacggct tccagcgcct tgagcgggtc gatctcggtc acgatgacgt
     1201 tggcgcccag gccacgcgcg cgcatggcga tgccgcgact gcaccagccg taaccggcga
     1261 cgacgacgtt cttgccggcg aggagtacgt tggtggcacg gataatgccg tccatcgtcg
     1321 attggcctgt gccgtagcga ttgtcaaaca tgtgcttggt cagcgcatcg ttgacggcca
     1381 tcatcgggaa ctgcaacgcg ccgtcgcggg ccatggcccg caggcgaatg atgcccgtgg
     1441 tggtttcttc ggtgccgccc agcatattat cgatcagatt gcggcggctc ttgtggatcg
     1501 tgctgaccag gtcggcgccg tcgtccatga cgatgtgggg ctggtggtcg agcacggcgt
     1561 tgatgtgctt gaagtaagtc tcgttgtcct cacccttaat ggcgtagacc gggatctcat
     1621 agtgactgac cagcgccgcg gctacgtcgt cctgggtact cagcggattg ctggcgctca
     1681 gcacaacatc cgcgccgccc gcctgcagac taaccatcag gttagccgtc tcggtggtca
     1741 catgcatgca cgcgctgatg cgcagaccgg caaacggccg atcgttctta aactgcttca
     1801 gcacgccacg taaaaccggc atttcctgct cggcccagtc gatgcgatgt cgcccgccgg
     1861 gcgcgagatc gatatttttg acgtcaaagt ccattgcgat tcgtaactcc ttaaatggcc
     1921 tgcccgtgat cctagtggac gacgggtgca gacgctttga ttgtttcggg ttcctgcagg
     1981 agcacgtcga gcgccccttc gtcatcaaat gattcccggt atcgcgcgac gaactcctcg
     2041 cgcgtaaagt agtgattttg tgggccggat tgctccagca cataagcagc cgccagcgca
     2101 cccatgcgcc cggccacgcc ccacggcaac cccaactgca tgccgcgcat caggccggcg
     2161 cgataggcat cgcccgcccc ggtcggctcg gccacccagc gtgtcggcgc gacggggacg
     2221 aactcttcgc ggccgtcgac gaccaatcgg gaaccggaag gccccatcgt cattaccaga
     2281 ccacccgctc gatccagaat ttcggcctcg ctcaggccgg ttttttcctg aataagctgg
     2341 aattcgtact cattgctgga cagcaaatag caaccgtcga taccttccag tatctcttca
     2401 gcgctgatgc gtgccgtctg ttggctgggg tcatagataa acggcaagcc gatgcttttg
     2461 cattcgcgaa tgtagctgcg catcgcgccg ggatcgttgg gggtgatgat ggtcagttca
     2521 gcatcgggcg catagcgggc gaacgacagc ttcccggcat atgccattgc cccggtatag
     2581 aagctggcga tctggttgtt gtcctcgtcg gtgttgacga aaaacgatgc gcaaaactca
     2641 tcctcgatgt cgagaatggc cgacgtgtcg atgccgtggc tgtcgagaaa tgcccggtac
     2701 tcgccgaaat
//
LOCUS       DAOMQG010000430         2709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15102_length_2709_cov_8.783346, whole genome shotgun sequence.
ACCESSION   DAOMQG010000430 DAOMQG010000000
VERSION     DAOMQG010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15102_length_2709_cov_8.783346"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1889
                     /gene="ftsH"
                     /locus_tag="PLC06_15535"
     CDS             6..1889
                     /gene="ftsH"
                     /locus_tag="PLC06_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRO91911.1"
                     /translation="MWSYSSAPAAQTDIPISRLAQEIHSDKVAKLIVSGNGREATVIY
                     EDQNRPPARVSISGVSSIEELLSAYGIQSESYSDGKPVVVYERPSQWNGILNLVGIFL
                     PAILIIGFFYFIFRQAQGNNSQAMSFGKSRARMFTGDHPTVGFDDVAGCDEAKEELRE
                     IVEFLKEPEKFVSFGARIPKGVLLVGSPGTGKTLLAKAVSGEAGVPFFSIAGSEFVEM
                     FVGVGASRVRDLFEQAKRNSPCIIFVDEIDAVGRHRGAGLGGSHDEREQTLNQILVEM
                     DGFDTDTHVIILAATNRPDILDPALLRPGRFDRRVVLDRPDMRGREAIFRVHLRGKPV
                     ANAVDINVLAKATPGFVGADIENTVNEAALLAARRNKKNIGMSEFQEAIERVQLGPER
                     RSRIMSPEEKELTAYHETGHAMVSHYLPHAQALRKITIIPRGMAGGVTWYMDEDTSFM
                     TRSKFEAMIATALGGRVAEEIVFGDITTGASNDLQQVTRMARAMVTQYGMSNELGLRV
                     YGEKQELVFLGREISEQRDYSDAIAEQIDAEVRRIIDVAHQFATDVLTQHRDQLELIA
                     RKLLEVETLEAAEFVALLEGKEYESPPSSPGSSKVTPSQKPAEASERSRPSLDMPPSP
                     TPA"
     gene            1929..>2709
                     /locus_tag="PLC06_15540"
     CDS             1929..>2709
                     /locus_tag="PLC06_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91912.1"
                     /translation="MFDSGEIARVLGYVNDVLQSIIVIFGAAVVLYNVPRLRRSVVAR
                     VFATVITFVVLVYLTELLVSRAVAADSAEILLRTGWIGVAMVPAAQFHLSDSLLTTTG
                     KISRRRHVLVILGYLTGILFVLLGLFSDLVVGPPTSSMVVPRLTSGPAFPLFAGYFWL
                     VALSSVYNVWRARQRCMTSTTRRRMTTTLFAFLAAPLGVFPYMLLGGDPTADMPLLLF
                     PVLIVGNLAVSLLFAVLTSQLVYVGSASPDRVVRVRLYKYMA"
BASE COUNT          514 a          771 c          797 g          627 t
ORIGIN      
        1 ccgtcttgtg gagctatagc tcggctccgg cagcccaaac cgatatccct atcagtcggc
       61 ttgctcaaga aattcattcg gataaagtcg ccaagctaat cgtctccggt aatggccgcg
      121 aggcgactgt tatctacgag gaccagaacc ggccgcccgc gcgggtcagt atcagcggcg
      181 taagctcaat tgaagagctg ttgtctgcgt atggcatcca gagcgagagt tattctgatg
      241 gaaagccggt cgtcgtttac gaacgtccca gccaatggaa cgggatcctc aatctggtcg
      301 gcatctttct cccggcaatc ctcatcatag gcttcttcta ctttatcttc cggcaagcgc
      361 agggtaataa cagccaggcg atgtcattcg gcaaaagccg agcgcgcatg ttcaccggcg
      421 atcatcctac cgtgggtttc gatgatgtgg ccggttgcga tgaggccaag gaagagttgc
      481 gggaaatcgt tgagtttctg aaagagccgg agaaatttgt cagcttcggc gcgcgcatcc
      541 cgaaaggtgt cttgctggtt ggcagccccg gcacgggcaa gaccttgctg gccaaggctg
      601 tgtccggtga agccggcgtg ccgttcttct ctattgccgg ttccgaattc gtcgaaatgt
      661 tcgtcggcgt cggtgccagc cgggtccgtg acctcttcga gcaggccaag cgtaacagcc
      721 cgtgcattat cttcgtggat gagatcgacg cagtggggcg ccatcgcggt gcgggtctcg
      781 gcgggtctca tgacgagcga gagcaaacgc tcaatcaaat cctggttgag atggacgggt
      841 ttgataccga cactcacgtc atcattctgg cggcgacaaa ccggccggat attctggacc
      901 ccgcgcttct tcgcccgggt cgcttcgatc gccgtgtcgt gctggatcgt cctgatatgc
      961 gcggccgcga agcaatcttc cgcgttcatt tgcgcggcaa accagtcgcc aatgcggtgg
     1021 atatcaatgt gttggccaaa gcaacgcccg gttttgtcgg cgcggatatc gaaaacaccg
     1081 tcaacgaggc tgccttgctg gccgctcgtc gcaacaagaa aaatatcggc atgagtgagt
     1141 tccaggaggc catcgaacgc gtccagctcg gccccgaacg ccgcagccgg atcatgtcgc
     1201 ccgaggaaaa agaactgacc gcctaccatg agacagggca cgcgatggtg agccattatt
     1261 tgcctcatgc ccaggcgtta cgcaagatca ccattatccc gcggggcatg gccggtggcg
     1321 tcacttggta catggacgag gataccagct tcatgacccg ctccaagttc gaggccatga
     1381 tcgccactgc cttgggcggc cgtgtggcgg aagagatcgt cttcggtgat atcaccacag
     1441 gggccagcaa tgatttgcag caggtgacgc gaatggcgcg cgcgatggtg acccagtatg
     1501 gcatgagcaa tgagttgggt ttgcgcgtgt atggcgagaa gcaggaactc gtgtttcttg
     1561 gccgggaaat cagcgagcag cgtgattatt ccgacgcgat tgccgagcag attgacgctg
     1621 aggtgcgccg gatcatcgac gtggctcatc agttcgcgac agatgtcctg acgcaacatc
     1681 gggaccaact ggagcttatc gcccggaaat tgcttgaggt ggaaactctg gaggcggccg
     1741 agtttgttgc cttgctggaa ggcaaggagt atgagtcacc cccctcttcc ccagggagct
     1801 cgaaagtcac cccatcccaa aaaccggcag aggcgagtga gcgctcccgt ccatctttgg
     1861 acatgccgcc ttcgccgacg ccggcttgat gatcctgccc atggcgtggg tgacgcccaa
     1921 atacgaccat gtttgattcc ggcgaaattg cgcgagtgct tggctatgtc aatgatgttt
     1981 tacagtccat cattgtcata tttggcgcag cggtcgtcct ctataacgtc ccgcgtttga
     2041 ggcgtagtgt tgtcgcaagg gtgtttgcta cggtaatcac ctttgtcgtg ctcgtttatc
     2101 tgacagagct tttggtcagc cgcgcggtgg cggccgactc agccgagata ttgttgcgca
     2161 caggctggat cggcgtcgcc atggttcccg ccgctcagtt ccacctgtcc gattccctgc
     2221 tgacaacgac cggcaaaata tcgcggcggc gacatgtgct ggtcattttg ggctatttga
     2281 ccggcatcct ttttgtcctg ctgggtctat tcagcgatct cgtcgtcggg ccgccgacat
     2341 ccagcatggt cgtaccccgc ctgacgagtg ggccggcctt cccgcttttt gccggttatt
     2401 tttggcttgt cgccttgtcc agcgtctaca atgtgtggcg agcgcggcaa cgatgtatga
     2461 cctcaacgac gcgccggcgc atgaccacca ctttatttgc cttcctggcc gcgccgctgg
     2521 gcgtcttccc ttacatgctg ttagggggcg acccaaccgc tgatatgcct cttttgctct
     2581 ttccggtgct catcgtcggc aatctggcgg tcagcctttt gtttgccgtc ctgacctctc
     2641 aattggtcta cgtcggctca gcttctcccg accgtgtggt tcgcgtacgc ctgtacaaat
     2701 acatggcgc
//
LOCUS       DAOMQG010000431         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15144_length_2704_cov_4.467724, whole genome shotgun sequence.
ACCESSION   DAOMQG010000431 DAOMQG010000000
VERSION     DAOMQG010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15144_length_2704_cov_4.467724"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLC06_15545"
     CDS             complement(<1..559)
                     /locus_tag="PLC06_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91913.1"
                     /translation="MQNQVHTKGLCSPTCQVLILLGCLLLFAAVITARAWLSDDAYIT
                     FRVVDNAIDGRGLTWNPDERVQAYTHPLWLFLLIAGRAATGELFYTATLLGIGLSVAA
                     VGLLAFRLPLRPTTAALGVLVLAGSRAFTDYATSGLENPLTHLLLALFLLAYLIRPAS
                     NRRLLTLSFLAALVALNRTDALLLVL"
     gene            610..2490
                     /locus_tag="PLC06_15550"
     CDS             610..2490
                     /locus_tag="PLC06_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91914.1"
                     /translation="MRSSIASRRPPFPVIAAIIALLLTTLYIMGLLVDWPGWLRGVNW
                     VWVRRVPSPGWRFVLMALALLVGLGLGWVALAKGDWPRRRTALFLAALVVLTPLLQIA
                     VAAQHRVQPLSVAVMSAAGFWHEGVRIDDPRAFLRDYPALMPDFADVHVRTQPPGQLL
                     AHRWLAAAYERLPGAADVVGRRLHRYDCAAPQLSGLDPAQLAAGSLRIALLFLSGLGA
                     PLLYAIGRRFCRPRAARLAAVGFAFLPGLLVFSGRMDVTFALLGLVMVWAALKAILDG
                     RRVAAVALALLVAITSFFSFTALALAGFALLLAGACALDAPPRRDAARRWLLTAALTG
                     AAVVLFWGALRLFFAVDGLAIWRTSQELHRYLRLEYPLWPLFNLYDLAAFMGWVPFVG
                     ALGVVGAAVVARRRGRHVPGGALALGWAAAVLLLNLSGTVRAETGRLWLFLMPVGLLV
                     GWAYFETHFDGKAGRRRLMLLLAGFMAQSLVMGYFLGGRAPDPATSPPQFRLPATMTP
                     LDYRLGEAITLRGYNIEATGDGTRLTLYWRAADFPRADYSVFVHALDGDGAILAQSDG
                     PPAAVPIWCWIPGEVVADERLLAVPGIREIAVGVYDPSSGARLPVSPPVGDDRIILPV
                     DD"
BASE COUNT          341 a          896 c          974 g          493 t
ORIGIN      
        1 gcagcaccag cagcagcgcg tcggtgcggt tgagcgccac gagggcggcc agaaaggaga
       61 gcgtcagcag ccggcgattg ctcgcggggc ggatcagata ggccagcagg aacagggcca
      121 gcagcagatg ggtcagcggg ttttcgaggc ctgatgtggc gtaatcggtg aacgcccgcg
      181 agccggccag gacgaggacg cccagcgcgg ccgtcgtggg gcgcagcggc aggcggaagg
      241 cgagcagccc gacggcggcc accgacaacc caatgcccag cagcgtggcc gtgtaaaaca
      301 gttcgcccgt ggccgcccgc ccggcgatga gcaggaacag ccacaagggg tgggtgtaag
      361 cctgcacgcg ctcgtcgggg ttccacgtca ggccgcgccc gtcgatagca ttgtcaacta
      421 cgcgaaaggt gatgtaggcg tcgtcgctga gccaggcgcg ggcggtgatg acggcggcaa
      481 acaggagcag gcagccgagg aggatcaaga cctgacaggt gggcgaacaa agcccttttg
      541 tatgcacctg gttctgcatc gcccacctgt caggtctatt agcaccaatc atgctagaat
      601 cgtggctcca tgaggtcgtc aatcgcaagc cgtcgtccgc cgtttcctgt catcgcggcg
      661 attatagcac tcctgctcac cacgctctat atcatggggc tgcttgtcga ttggcccggc
      721 tggctgcgcg gggtcaactg ggtgtgggtg cggcgcgtgc cttcgccggg gtggcgcttc
      781 gtcctgatgg cgctcgcgct gctcgtcggg ttggggttgg gttgggtggc gctggccaag
      841 ggcgactggc cccggcggcg gacggcgctc tttctggcgg cgctggtggt gttgacgccg
      901 ctgctgcaaa tcgccgtggc cgcccagcat cgcgtccagc cgctcagcgt ggcggtcatg
      961 agcgccgccg ggttctggca cgagggggtg cgcatcgacg acccgcgcgc cttcctgcgc
     1021 gactatccgg cgctgatgcc cgacttcgcc gacgtccatg tgcgcacgca gccaccgggc
     1081 caactgctgg cccaccgctg gctggcggcc gcctatgagc ggctgcccgg cgcggccgat
     1141 gtcgtcggcc gccggctgca tcgctacgac tgcgccgcgc cgcagctcag cggcctcgac
     1201 ccggcgcagc tggcggcggg cagcctgcgg atcgcgctgc tgttcctcag cgggctaggc
     1261 gcgccgctgc tctacgccat cggccgccgg ttctgtcggc cgcgggcggc gcgactggcg
     1321 gcggtggggt tcgcctttct gccgggcctg ctcgttttct ccggccggat ggatgtcacg
     1381 tttgcgctgc tgggactggt catggtgtgg gcggcgctga aggcgatact cgacggccgt
     1441 cgcgtcgcgg ccgtcgcatt ggcgctgctg gtcgccatca cttccttctt ctccttcacg
     1501 gcgctggcgc tggcggggtt tgccctgttg ttggccggag cctgcgccct cgacgcgccg
     1561 ccgcggcggg atgccgcgcg ccgctggctc ctgacggccg cgttgaccgg ggcggccgtc
     1621 gtcctgttct ggggcgcgct gcgcttgttc ttcgccgtcg acggcctcgc catctggcgc
     1681 acaagccagg agcttcaccg ctatctgcga ttggagtacc cgctctggcc gctgttcaac
     1741 ctgtacgatc tggcggcgtt catgggttgg gtgccgtttg tgggcgcgct gggggtggtc
     1801 ggggcggccg tcgtcgcccg gcggcggggg cggcatgtgc ccggcggcgc gttggcgctg
     1861 ggctgggcgg cggccgtgct gctgctcaat ctgtcgggca cggtgcgggc ggagacgggg
     1921 cggctgtggc tgttcctcat gccggtgggg ctgctcgtcg gctgggcata ttttgagaca
     1981 catttcgacg gcaaggccgg gcggcgccgg ctgatgctgc tgctggccgg gttcatggcc
     2041 cagtcgctgg tgatgggcta cttcctgggc ggccgcgcgc ccgaccccgc cacgtccccg
     2101 ccgcaattcc gcctgccggc gacgatgacc cccctcgact accgtctggg cgaggccatc
     2161 actctgcgcg gctataatat tgaagcgacc ggcgacggga cgcggctgac gctctattgg
     2221 cgggcggccg atttcccccg cgccgattat tcggtcttcg tccacgcgct cgacggcgac
     2281 ggggcgatcc tcgcccagag cgacggcccg ccggcggccg tgcccatctg gtgctggatc
     2341 cccggcgagg tcgtggccga cgagcggcta ttggccgtcc ccggcatccg cgagatcgcc
     2401 gtgggcgtct atgatccgtc gagcggcgcg cgcctgccgg tttcgccgcc ggtgggggat
     2461 gaccggatca tcctgccggt ggatgattag acctgacagg tgggcgcggt gacgctcagg
     2521 acagaccaga gaccgggtgc gcccacctgt caggtcttgt tgcttcgggg ccaccagtgc
     2581 ccccttcccc ctttcccccc ttctccccaa ttcgtaattc gtaattcgta attcgtaatt
     2641 cacccctact cccactcaat cgtccccggc ggcttcgagg tgatgtcgaa caccacccga
     2701 ttga
//
LOCUS       DAOMQG010000432         2697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15203_length_2697_cov_10.077214, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000432 DAOMQG010000000
VERSION     DAOMQG010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15203_length_2697_cov_10.077214"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /gene="fabF"
                     /locus_tag="PLC06_15555"
     CDS             complement(<1..1232)
                     /gene="fabF"
                     /locus_tag="PLC06_15555"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRO91915.1"
                     /translation="MTNQDGARRRVVVTGVGLITPLGHNVPDTWAAILGGKSGLGPIT
                     LVDPADHTIGGLCEVKEFDPANYMDRKDARRRDRYEQLATVAANEAMRHSGLTITDDN
                     RERVGITLGTGVGGMRTVIEQETIRRESGLRRLSPFAITMIMPNGAAGMLAIDYGIQG
                     PSTTITTACAAGNDAVGHAYRAIRYGELDAVLTGGSECIMTSVAIGGFERAGATSSKS
                     SEAPQPFDANRDGLVIGEGAGMLVLESLEHAQARGATILAEIAGYGQTTDAFHITAPP
                     DDGSGAARAIRKALADAGLRPEDVDYISAHGTATPLNDAAETLAIKAALGEHAWRVAV
                     SSTKSMTGHIMGATGAIESVFCALAIRDQMLPPTINYSTPDPACDLDYVPNVARPGRV
                     RVCLNNAFGFGGHNAVTV"
     gene            complement(1259..2002)
                     /locus_tag="PLC06_15560"
     CDS             complement(1259..2002)
                     /locus_tag="PLC06_15560"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HRO91916.1"
                     /translation="MEIEIRLFATLKDRAGSDRIRVALPANPTTVAALLDAVGADYPA
                     LAPALGSALVAVNRAFAGSETPVGAADEVAIFPPVSGGSPPSGGAADPAQPYPTYFAV
                     TDAPLDIEAIHARLTGPEIGAVVSFTGFVRGRTRRDGLPPQTVRLDYEAYESMAVEKM
                     AQIAREIWARWPEVRGVALVQRLGRLGVGENTTFVACAGAHRDVGVFEAARYGIDRLK
                     EIVPVWKKEIGADHSEWVEGHYRPTTEDN"
     gene            complement(2146..2643)
                     /gene="moaC"
                     /locus_tag="PLC06_15565"
     CDS             complement(2146..2643)
                     /gene="moaC"
                     /locus_tag="PLC06_15565"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRO91917.1"
                     /translation="MTNENKPAGLTHLDESGRARMVDVGDKAVTERVAVARGRVVMQP
                     ETLRLIIEGNMKKGDVLAVAQLAGIMAAKRTSELIPLCHPLLLNRIDVVCAPNLAENA
                     VDIEATVRVSGKTGVEMEALTAVTVAGLTLYDMAKAVDRGMRLTDVRLVSKSGGRSGE
                     WVSGE"
BASE COUNT          432 a          929 c          863 g          473 t
ORIGIN      
        1 atgaccgtga cggcattgtg gccgccgaag ccaaaggcgt tattgaggca aacgcgcacg
       61 cggccgggcc gggcgacgtt gggcacatag tccaggtcgc aggccgggtc gggcgtcgaa
      121 tagttgatgg tcggcggcaa catctggtcg cgaatggcca gcgcgcaaaa cacgctctcg
      181 atggccccgg tcgcgcccat gatgtggccg gtcatcgatt tggttgaact gacggccacg
      241 cgccaggcat gctcgcccag cgccgccttg atggctaatg tctcggccgc gtcattgagc
      301 ggcgtcgccg tgccgtgggc gctgatgtag tccacgtctt ccggccgcag cccggcgtcg
      361 gccagcgcct tgcggatggc gcgcgccgcg ccggagccgt catcgggcgg cgcggtgatg
      421 tggaaggcgt cggttgtctg cccatagccg gcgatctcgg ccaggatcgt cgccccgcgg
      481 gcctgggcat gctccaggct ttccagcacc agcatccccg ccccctcgcc aatgaccagc
      541 ccgtcccggt tggcgtcgaa cggctgcggc gcttcgctcg acttcgacga agtcgccccc
      601 gcccgctcga agccgccaat ggcgaccgag gtcatgatgc actccgaccc gccggtcaga
      661 accgcgtcca gttcgccgta gcgaatggcg cgataggcgt ggcccacggc gtcattgccc
      721 gcggcgcagg ccgtggttat cgtcgtcgat gggccttgaa taccatagtc gatggccagc
      781 atccccgccg cgccgttggg catgatcatg gtgatggcga acgggctgag gcggcgcaag
      841 ccgctctcac gccgaatcgt ctcctgctcg atgaccgtgc gcatgccgcc gacgcccgtg
      901 cccagcgtta tgccgacgcg ctcgcggttg tcgtcggtaa tcgtcaggcc cgaatggcgc
      961 atggcctcgt tggcggccac cgtcgccagc tgctcatagc ggtcgcggcg gcgggcgtcc
     1021 ttgcgatcca tatagttcgc cgggtcaaac tccttgacct cgcacagtcc gccgatggta
     1081 tggtcggcgg gatcgaccag cgtgatgggg ccgaggcccg acttgccgcc cagaatagcg
     1141 gcccaggtat cgggcacgtt gtggcccagg ggggtaatca accccacgcc ggtcaccacc
     1201 actcgccgtc gcgctccgtc ttgattcgtc atatcttcac tccaggaaga gatttatgct
     1261 aattgtcctc ggtcgtcggc cgataatgac cctcgaccca ctcgctgtga tcggccccga
     1321 tctccttctt ccacaccggc acgatctcct tcaaccgatc gatgccatag cgggcggcct
     1381 cgaaaacgcc cacgtcgcga tgtgcgccgg cgcaggcgac gaacgtcgtg ttctcgccga
     1441 cgcccagccg gccgagccgc tggaccagcg ccacgccgcg cacctcaggc cagcgcgccc
     1501 atatctcgcg ggcgatctgg gccattttct ccacggccat ggactcatag gcttcatagt
     1561 cgagacgcac cgtctgcggg ggcaggccgt cgcgccgggt gcggccgcgc acgaagccgg
     1621 tgaagctgac caccgcgccg atctccggac cggtcaggcg ggcatggatg gcctcgatgt
     1681 cgagcggcgc gtcggtcact gcgaagtacg tcgggtacgg ctgggcgggg tcggccgcgc
     1741 cgccgctcgg cgggctgcca ccgctcacgg gcgggaagat tgccacctcg tcggccgcgc
     1801 ccaccggcgt ctccgacccg gcaaaggcgc gattgacggc caccagcgcc gaccccaacg
     1861 ccggagccag cgccggataa tcggccccca cggcgtcgag cagggcggcc accgtcgtcg
     1921 ggttggccgg cagagcgacg cgaatgcggt cgctccctgc ccgatccttc aacgtggcaa
     1981 acagacgaat ctcgatctcc ataagcaaat gtcgtttagt agtaaccctc aagccgttag
     2041 tcggatgcgg gtcatgagca gcgagtaaat ggttagcaat gttcagtagg tcagtaggtc
     2101 agtggaacga tcgcggccgc cggtcggcgc ttaattcgcc actcgtcatt cgccgcttac
     2161 ccactctccg cttcgcccac cggacttgct gaccagtcgc acgtcggtca ggcgcatgcc
     2221 ccggtcgacg gccttggcca tgtcatagag ggtcagcccg gccacggtga cggccgtcaa
     2281 cgcttccatc tccacgcccg tcttgcccga cacgcgcacg gtcgcctcga tatctaccgc
     2341 attttcggcg aggttgggcg cgcaaactac atcaatccgg ttcaacaaca gcggatgaca
     2401 gagcgggatc aactcgctcg tccgcttggc ggccatgatc ccggccagtt gggccacggc
     2461 cagcacgtcg cccttcttca tattgccttc gatgatgagg cgcagcgtct ccggctgcat
     2521 gacgacgcgg ccgcgggcca cggccacacg ctcggtcact gccttgtcgc caacgtcgac
     2581 catgcgggcg cgaccgctct cgtcgaggtg ggtcagcccg gccggtttgt tctcgttcgt
     2641 catacaggag atattatagc cattgtggcc ggcaatggac acaagcgggc tattctg
//
LOCUS       DAOMQG010000433         2690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15241_length_2690_cov_9.585579, whole genome shotgun sequence.
ACCESSION   DAOMQG010000433 DAOMQG010000000
VERSION     DAOMQG010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15241_length_2690_cov_9.585579"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLC06_15570"
     CDS             complement(<1..203)
                     /locus_tag="PLC06_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91918.1"
                     /translation="MKYIATVKDKEYTIEIDPDRGILIDDVPTEIDFRRLPSGGVSSL
                     LMNHRSISAVIEEVGDYWDVLIEG"
     gene            complement(222..1745)
                     /gene="accC"
                     /locus_tag="PLC06_15575"
     CDS             complement(222..1745)
                     /gene="accC"
                     /locus_tag="PLC06_15575"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRO91919.1"
                     /translation="MERTDGLLVKKFDKLLIANRGEIAMRILRAARELGIATVAVYSD
                     ADRDAPHVRFADEAYHIGPPAARESYLVVEKLLDVARRSGAEAIHPGYGFLAERETFA
                     QACVDAGIVFIGPPARAIGIMGDKLMARQTVIAAGVPVVPGTLPGQTDEEMTAAALRM
                     GFPVLVKAAAGGGGKGMRPVHRAEDLADAFAGARREAKAAFGDDTVYIEKMITEARHI
                     EIQLLADSHGNTLYLGERECSLQRRHQKLIEEAPSFIVDADLRRRMGEVAVAAAKAVD
                     YVNAGTIEFLVDKEKNFYFLEMNTRVQVEHPVTELVTGIDIVQEQLRIARGRRLRIRQ
                     EDVRIKGWAIECRINAEDPYNNYLPSTGTITASHLPTGPGVRVDTGVYVGNAISPYYD
                     SMISKLICYGETRGEAVLRMRRALEEYRVLGVKTNIPFHQHMMDSHRFLSGQFDTNFV
                     EERFSMDDREAPRALEAALLATLATHEQYQRAAQIVAPGDRDTSNWKWYSRWERLHR"
     gene            complement(1761..>2690)
                     /locus_tag="PLC06_15580"
     CDS             complement(1761..>2690)
                     /locus_tag="PLC06_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRO91920.1"
                     /translation="VTFEELGGAMTHNKISGVAHMAAESEEDCLFLVREMMSYLPSNN
                     MEDAPFKAATDDTLRAEEKLNTIVPDSPNKPYDIRDAINLIVDDGRFYEIQEHYAQNI
                     VVGFARLGGFSVGIVANQPMVLAGVLDIDASEKAARFVRFCDCFNIPLIVFEDVPGFL
                     PGIDQEHGGIIRHGAKLLYAFCEATVPKLTVITRKAYGGAYCVMNSKHIRSDFNVAWP
                     EAEIAVMGPEGAVNIIYRREISEAEQPDARRVELVDEYRDHLANPYIAAQRGYIDDVI
                     EPSETRPRLINALNMLQNKRDQNPPKKHGNMPL"
BASE COUNT          534 a          841 c          761 g          554 t
ORIGIN      
        1 ccttcgatga ggacgtccca atagtcgccc acttcctcga taacagccga gatagaacga
       61 tgattcatga gaagcgacga cacgcctccc gaaggcaggc ggcggaaatc gatctcggtg
      121 ggcacatcat caatgaggat gccccgatca ggatcgatct caatcgtgta ctctttatcc
      181 ttaacggtcg cgatatattt cataatgccc ttctcctctg cctagcgatg caaccgttcc
      241 cagcggctat accatttcca gttgctggtg tcgcgatcac cgggggcgac aatctgcgcc
      301 gcgcgctgat actgttcgtg cgtcgccagc gtcgccagga gcgccgcctc cagagcgcgg
      361 ggcgcctcgc gatcatccat gctgaaccgc tcctcaacaa aattggtgtc gaactggccg
      421 gagagaaagc ggtggctgtc catcatatgt tgatggaatg gaatattggt cttgacgccc
      481 agaacccggt attcctccag cgcccgccgc atccgcaaaa ccgcctcgcc gcgagtctcg
      541 ccatagcaga tgagcttgct aatcatcgaa tcatagtaag gggaaatcgc attgccgaca
      601 tagacaccgg tgtccacacg cacgccgggg ccggtgggca ggtgcgacgc cgtaatcgtc
      661 ccggtggacg gcaaatagtt gttgtagggg tcttcggcgt tgatgcgaca ttcgatggcc
      721 cagcctttga tgcgcacatc ctcctggcga atccgcaacc ggcggccgcg cgcgatacgc
      781 agttgctcct ggacgatgtc gataccggtg acaagctccg tcaccggatg ctccacctgt
      841 acgcgcgtgt tcatctccag aaaatagaag tttttctctt tatcgaccag gaattcgatc
      901 gtacctgcgt tgacataatc gacggctttg gctgcggcta cagcgacttc gcccatgcgc
      961 cgacgcaaat cggcatcgac gataaaagat ggcgcttctt cgatgagctt ctgatggcgg
     1021 cgctggagcg agcattcgcg ttcacccaga tagagcgtgt tgccgtggct atcggccaga
     1081 agctggattt cgatatgacg cgcctcggtg atcatcttct cgatatagac cgtgtcgtcg
     1141 ccgaaggcgg ccttcgcctc gcgtcgcgcc ccggcaaacg catcagccag atcctccgcg
     1201 cgatgaaccg gacgcatccc cttgccgcca ccgccggccg ccgccttcac cagcaccggg
     1261 aagcccatgc gcagcgccgc ggccgtcatc tcttcgtccg tctgacccgg cagcgtaccg
     1321 ggcaccacgg gcactccggc agcgatgacc gtctgccgag ccatgagctt gtcgcccatg
     1381 atgccgatcg cccgcgccgg tgggccaatg aaaacgatgc ccgcatccac acaagcctgg
     1441 gcaaacgtct cgcgctcggc cagaaagccg tagccgggat gaatggcctc cgcaccagaa
     1501 cgtcgggcca catcgagcag cttctcgacc accaggtagc tttctctggc tgccggcggg
     1561 ccaatatgat acgcctcgtc ggcaaagcga acgtgggggg cgtcacggtc ggcgtcggaa
     1621 tagactgcta ccgtcgcaat gcccagctcg cgcgccgcac gcaggatacg catggcgatc
     1681 tctccgcggt tggcgatcag caatttatcg aatttcttaa ccaggagtcc gtctgttcgt
     1741 tccatcgcgc tccggccctc ctatagcggc atgttgccgt gcttcttggg cgggttctga
     1801 tcccgcttgt tttgcagcat attgagcgcg ttgatgagcc gtggtcgagt ctcgctcggc
     1861 tcgatcacat cgtcgatata accgcgctgg gcggcgatat aggggttcgc caaatggtcg
     1921 cggtattcat ccaccagttc gacccggcgg gcatccggct gctccgcttc gctgatttcg
     1981 cgccgataga tgatattgac cgccccttcc ggccccatga cggcgatttc ggcctccggc
     2041 caggcgacgt tgaaatcgga gcggatatgc ttgctgttca tgacgcagta ggcaccgcca
     2101 tacgccttgc gggtgatgac cgtcaatttg ggcaccgttg cctcgcaaaa agcatagagc
     2161 aatttggccc cgtggcggat gatgccgcca tgctcctgat cgatcccggg caggaagccg
     2221 ggcacatcct caaagacaat aagcggaatg ttgaaacagt cacagaagcg aacgaaccgc
     2281 gccgctttct cgctggcgtc aatatcgagg accccggcca gcaccatcgg ctggttggca
     2341 acgatgccga cgctgaaacc gcccaatcgc gcgaagccca cgacgatatt ctgggcgtaa
     2401 tgctcctgaa tctcataaaa gcggccatca tcgacgatca gattaatcgc gtcgcgaata
     2461 tcataaggct tgttcgggct atcgggcacg atcgtgttga gcttttcctc ggcgcgcaag
     2521 gtgtcatcgg tagcggcttt gaacggcgcg tcttccatat tgttcgaggg caagtagctc
     2581 atcatctccc gaaccaggaa caggcaatct tcctcggatt cggccgccat gtgggccacg
     2641 ccgctgatct tgttgtgggt catcgccccg ccaagctcct caaacgtgac
//
LOCUS       DAOMQG010000434         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15318_length_2679_cov_6.703887, whole genome shotgun sequence.
ACCESSION   DAOMQG010000434 DAOMQG010000000
VERSION     DAOMQG010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15318_length_2679_cov_6.703887"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..851
                     /locus_tag="PLC06_15585"
     CDS             99..851
                     /locus_tag="PLC06_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRO91921.1"
                     /translation="MLSIPELQARHAAVMERIAAAAARSGRDAADITLIAVTKTWPAE
                     LVLAACEAGMRRFGENRPEELAGKRAAVEAVLGPESGVVWHLIGPVQSRKSGLAAAHA
                     DVFHALDRERIARRLSADLAANGRQLPCFIEVNVSGEASKLGVDMRNWETDATQTAAL
                     RKLFTEAAALPGLQPIGLMTMAPWDAPPDEIRAVFRRTRELATRLWWELALVEPPALS
                     MGMTDDFEIAIEEGATHVRVGRALFGERERPL"
     gene            876..1139
                     /locus_tag="PLC06_15590"
     CDS             876..1139
                     /locus_tag="PLC06_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRO91922.1"
                     /translation="MILVDIINLVFGAFYVLLLAQIVLSWVRPDPWHPTWGPIIRFIN
                     GVIDPILNPIRRVMPPLGGLDFSPMIVLILARVLQGFLINLVV"
     gene            complement(1335..2498)
                     /locus_tag="PLC06_15595"
     CDS             complement(1335..2498)
                     /locus_tag="PLC06_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO91923.1"
                     /translation="MFDFITEEHKMIQGAARDFAQNSIAPIAAEFDESGEFPLDTIRQ
                     MGKLGFMGIEVPEEYGGVGMDTIAYVLAMVEICKADASHGTIMSVNNSLFAHGLMKFG
                     TEEQKQKYLVPVATGEKIGAYSLTEPMSGSDAGTMKSRAVLNEAGSHYVINGRKSWVT
                     SGPHADTIVLFTMTQPEKKHRGVSAFIIETDKPGFIRGKKEPKLGIRASATSEIIFDN
                     YECPVENRIGAEGDGFKIAMTVLDAGRIGIASQALGIAEAALEASIAYAKEREAFGQK
                     IGEFQMIQQKLADMKCRVDASRLLIYQAALAKIAAAKTGGRYSMEASMAKLFASETAM
                     WVTTQAIQIHGGMGYSKEMPLERYFRDAKITEIYEGTSEIQRMVIARSLTGLR"
     gene            complement(2491..>2679)
                     /locus_tag="PLC06_15600"
     CDS             complement(2491..>2679)
                     /locus_tag="PLC06_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91924.1"
                     /translation="LSNSLLQTKEARYWLAERRRWEYGIELTPEDFATLLDYADRGAS
                     EPTSPTNRAAMAQEEPHV"
BASE COUNT          434 a          861 c          841 g          543 t
ORIGIN      
        1 gcggggagtg atgagatacg aatgacgaga ggcggctcct gttgcccggc acgcttgctt
       61 cgtttttcat tttccccaat ccgacggata atccccccat gctttccatc cccgaactgc
      121 aagctcgcca cgcggccgtc atggagcgca tcgccgccgc cgccgcgcgg agcggccgcg
      181 acgcggccga tatcaccctg atcgccgtca ccaagacctg gccggccgag ctggttcttg
      241 ccgcctgtga ggccgggatg cgccggttcg gcgaaaaccg gccggaggag ctggccggca
      301 agcgggccgc cgttgaggca gtcctcggtc cggagagcgg cgtcgtctgg catctcatcg
      361 gcccggtgca gagccgcaag tccggtctgg ccgcggccca cgccgacgtg ttccacgcgc
      421 tggatcgcga gcggatcgcc cggcggctgt cggccgacct ggctgccaac ggccggcaac
      481 tgccctgctt catcgaggtc aacgtctccg gcgaggcgtc gaagctgggc gtggacatga
      541 ggaactggga gacggatgca acgcaaacgg ccgcgctgcg taaactgttc acagaggcgg
      601 ccgcgctccc cgggttacaa cccatcggcc tgatgaccat ggccccgtgg gacgcgccgc
      661 ccgatgagat tcgcgccgtc tttcgccgca cgcgcgaatt ggcaacccgc ctatggtggg
      721 agttggcgct ggtagaaccg cccgcgctgt cgatgggcat gaccgacgac ttcgagatcg
      781 ccatcgagga aggggccacc cacgttcggg tcggccgcgc cttgttcggc gagcgggagc
      841 ggccgcttta atcgcagaaa ggaatacaaa ccggcatgat actagtcgat ataatcaatt
      901 tggtctttgg cgctttttat gtgttgctgc tggcccagat cgtcctgtcc tgggtgcggc
      961 ccgacccctg gcacccgacc tgggggccga tcattcgctt catcaacggg gtgatcgacc
     1021 ccatcctcaa cccgattcgc cgggtgatgc cgccgctggg tggcctcgat ttcagcccga
     1081 tgatcgtgct gattttggcg cgggtgctgc aggggttttt gatcaatctg gtggtataag
     1141 gggaggaacc cgcggatttg agagggtggt cagtgggtcg gtaatcagta gatcagtaga
     1201 tcagtagatc agtaatcagt agatcagtag gtcagtaatc actgacccac tgacctactg
     1261 ccccactgcc ccactgaccc actgacccac tgacccactg acccactgac ccactgaccc
     1321 actgatcact ctctttacct cagcccggtg agcgaccgcg caatcaccat gcgctgaatc
     1381 tcgctcgtcc cttcgtagat ctcggtgatc ttcgcgtcgc ggaagtagcg ctccagcggc
     1441 atctccttgc tatagcccat gccgccgtgg atctggatgg cctgggtggt cacccacatg
     1501 gccgtctcgc tggcgaacag cttggccatg ctggcttcca tcgagtagcg gccgcccgtt
     1561 ttggctgcgg cgatcttggc cagcgccgcc tgatagatga gcaggcggct ggcatcgacg
     1621 cggcacttca tgtcggccaa tttctgctgg atcatctgga actcgccgat cttctgcccg
     1681 aacgcctcgc gctccttggc ataggcgatg ctggcttcga gggcggcctc ggcgatgccc
     1741 agcgcctgcg acgcgatgcc gatgcgcccg gcgtcgagca ccgtcatggc gatcttgaag
     1801 ccgtcaccct cggcgccaat gcggttctcg accgggcact cgtagttgtc gaagatgatc
     1861 tcgctggtgg cgctggcgcg gatgcccagc ttcggctcct tcttgccgcg gatgaagccc
     1921 ggcttatcgg tttcgatgat gaatgccgac acgccgcgat gcttcttctc cggctgggtc
     1981 atggtgaaca ggacgatggt gtcggcgtgg gggccggacg tgacccacga cttgcggccg
     2041 ttgatgacgt agtggctgcc ggcctcgttc agcacggcgc ggcttttcat cgtgcccgcg
     2101 tcgctgccgc tcatcggctc cgtcaggcta taggcgccga tcttctcccc cgtggccacc
     2161 ggcacgagat acttttgctt ctgctcttcg gtgccgaact tcatcaggcc gtgggcgaac
     2221 agcgagttat tgaccgacat gatcgtgccg tggctggcgt cagccttgca aatctcgacc
     2281 atggccagca cataggcgat ggtgtccatc cccacgccgc cgtactcctc cggcacctcg
     2341 atgcccatga agcccagctt gcccatctgg cggatggtgt ccagcggaaa ttcgcccgac
     2401 tcgtcgaact cggcggcgat gggagcgatg gagttttggg cgaaatcgcg cgccgcgccc
     2461 tggatcatct tgtgttcttc ggtaataaaa tcaaacatgg ggttcctcct gggccatggc
     2521 ggcccggttt gtgggtgaag tgggttcgct cgccccgcgg tcggcgtaat ccagcagcgt
     2581 ggcgaaatct tccggcgtca gctcgatgcc gtactcccag cgccgccgct cggccagcca
     2641 gtagcgggcc tctttcgtct gcagcaggct gttggacaa
//
LOCUS       DAOMQG010000435         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15319_length_2679_cov_6.453887, whole genome shotgun sequence.
ACCESSION   DAOMQG010000435 DAOMQG010000000
VERSION     DAOMQG010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15319_length_2679_cov_6.453887"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2011
                     /locus_tag="PLC06_15605"
     CDS             <1..2011
                     /locus_tag="PLC06_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91925.1"
                     /translation="WLPPWASNTISVVMHEMGHGFGLPHSSADYGETYDSPWDVMSKD
                     RANCAMATDPVYGCIGQGTISYHKDLLGWIAPGRKYTHQSGTQTITLEPLGAPGAGGY
                     LMAQIPIDGSDTHFYTIEARAQASYDAKLPGAGVIIHEVITTRQRPARVVDVDLNGNP
                     GDDGAIWTVGETFSGLDHVVVTVDGKTDDAWTVTIANAAPSAWTGAAVGVGAGGDLDD
                     DGAITLRGRGGDIFGAADSFYFAHQAATGSMELVGRITAWDAAGVKSGKAGLMIRGSL
                     DNDAPHFTIHLTGPKNTVKLKWRGAAGEQTATADLPGKIKLPAWLKIIKTGQTFAAFY
                     SAGGQAWTQIAPPVTLPGFPDAFHYGMAVTSNSPDKNAQATFAGVAATPWSTMAVGDG
                     SGGTTTEAGDAVRLWATGGDIYGTADSFLYYALPGQGDLDLRARLDDWDAGGIGSAKA
                     GLMIRGSAAAGAPEFAIHVTGPNNSIKLKVRTTAGGTTQGESGPSQPSLPLWLRLVKT
                     GNLVAAYYSTDGAYYVALGAPRALGGLGADYLYGLAATSNEPGRYVTADFQSARVGPL
                     PIPLAPTATATATPTPTATTTPTSTATATPTTTSTATATASPTATGTPIATGTPTATP
                     TGTRPATATATVSPTATASPTASPTVTRPPNTHYLVFLPSLFVGR"
     gene            complement(2105..2473)
                     /locus_tag="PLC06_15610"
     CDS             complement(2105..2473)
                     /locus_tag="PLC06_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91926.1"
                     /translation="MSWKASGKADLPLTGDDRKWDEEQAKKSIFKWAGWPDNPNPVKA
                     RQAFFAYDDDDGEEKQSYKLPFAAVVDDRLKAVPHALHAVAAVLEGARGGVDLPSGVI
                     SDVRGKVEAYYRKMDEKVPW"
BASE COUNT          484 a          917 c          858 g          420 t
ORIGIN      
        1 ctggctgccg ccgtgggcga gcaacaccat cagcgtcgtc atgcacgaga tgggccacgg
       61 cttcggcctg ccccattcgt cggccgacta tggcgagacc tacgacagcc cgtgggatgt
      121 gatgagcaag gatcgcgcca actgcgccat ggcgaccgat ccggtctacg gctgcatcgg
      181 ccaggggacg atcagctatc acaaggatct gctcggctgg atcgcgccgg ggcgcaaata
      241 tacccaccaa agcgggacgc agaccatcac gctggagccg ctcggcgcgc cgggcgcggg
      301 cggctatctg atggcccaaa tccccatcga cggttccgac acccatttct acaccatcga
      361 ggcgcgggcg caggcaagct atgacgccaa gctgcctggc gcgggggtca tcatccatga
      421 ggtgatcacc acccgtcagc ggccggcgcg cgtggtcgat gtcgatctga acggcaaccc
      481 cggcgacgac ggcgcgatct ggaccgtcgg cgagacgttc agcggattgg atcatgtggt
      541 ggtgaccgtc gacgggaaga cggacgacgc ctggaccgtg accattgcca acgccgcccc
      601 cagcgcatgg acgggcgcgg ccgtgggcgt gggggccggc ggcgatctgg acgacgacgg
      661 cgcgatcacg ctgcgcggcc gcggcggcga catcttcggc gcggccgaca gcttctactt
      721 cgcccatcag gcggccacgg gcagcatgga actggtgggg cgcatcaccg cctgggacgc
      781 ggccggggtc aagtcgggca aggcggggct gatgatccgc ggctcactcg acaacgacgc
      841 cccccatttc accattcatt tgacggggcc gaagaacacc gtcaagctga aatggcgcgg
      901 cgcggccggc gagcagacgg ccaccgccga cctgccgggg aagatcaagc tgccggcgtg
      961 gctgaagatc atcaagaccg gtcaaacgtt cgccgcgttc tattcggccg gcgggcaggc
     1021 atggacgcag atcgccccgc cggtgacgct gcccggcttc cccgacgcgt tccactatgg
     1081 catggcggtg acgtccaaca gccccgacaa gaacgcacag gccaccttcg ccggcgtcgc
     1141 ggccacgccc tggagcacga tggccgtggg cgacggctcc ggcggcacga cgaccgaggc
     1201 gggcgacgcc gtgcggctgt gggccaccgg cggcgacatc tacgggacgg ccgacagctt
     1261 cctctattac gccttgcccg gccagggcga cctcgatttg cgggcgcggc tcgacgactg
     1321 ggacgccggc ggcattggct cggccaaggc ggggctgatg attcgcggct cggcggccgc
     1381 cggcgcaccg gagttcgcca tccacgtgac cggaccgaac aacagcatca aactaaaggt
     1441 tcggacaacg gccgggggaa caactcaggg ggaaagcggc cccagtcagc cgtcgctgcc
     1501 gctgtggctg cggctggtga agacgggcaa cctcgtggcc gcctactatt cgaccgacgg
     1561 cgcctattat gtggccctcg gcgcgccgcg ggcgctgggc gggttagggg cggactatct
     1621 ctacggcctg gcggccacat ccaacgagcc ggggcgctat gtgacggccg atttccagag
     1681 cgcgcgcgtg gggccattgc cgatcccgct cgcgccgacg gcgacggcga ccgccacgcc
     1741 gacacctacg gcgacgacaa ccccaacttc aaccgcgacg gcgacgccta caacaacgag
     1801 tacggcgaca gcgacggcct cacccacagc cacggggaca cccatagcga cagggacacc
     1861 gacggcaacg ccaaccggca cgcgaccggc gaccgcgacc gcgacggtgt cgcccaccgc
     1921 aaccgcgtcg ccaaccgcgt caccgacagt cacgcggccg ccgaataccc actacctcgt
     1981 ctttctgccg tcattgttcg tcgggcgatg aggaatatag cccagcccac tgtggcgcaa
     2041 cctgtccagg ttgcctcttc gcggccgcaa gccggacagc ttgcgctaca ggcccggccc
     2101 caacctacca cggcactttc tcatccatct tcctatagta tgcctccacc ttgccgcgca
     2161 catcgctgat gacgccgctc ggcagatcaa caccgccgcg cgcgccctcg aggacggccg
     2221 ccacggcatg cagcgcgtgg ggcacggcct tcagtcgatc atcaacgacg gcggcaaacg
     2281 gcagcttgta gctctgcttc tcctcgccgt catcatcatc ataggcaaaa aacgcctgcc
     2341 tggccttgac cggattgggg ttgtcgggcc agccggccca tttgaagatc gattttttgg
     2401 cttgttcctc gtcccacttg cgatcgtcgc cggtcagggg gagatccgct ttgccgcttg
     2461 ctttccagga catgattttg ctccttgggg tcggcatgat tctccggctc catcatgaat
     2521 atctgcaatt tgattgtaga tcaatctgtc aagtccgggg tcgaattcgg ccgcaaacag
     2581 attgctgcgc ggcaacttct catctacagc atggctgaat agattaacgc gcctttacgc
     2641 agcagcggct ttgcgctaca ttccggcaac tttccggag
//
LOCUS       DAOMQG010000436         2644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15580_length_2644_cov_7.863654, whole genome shotgun sequence.
ACCESSION   DAOMQG010000436 DAOMQG010000000
VERSION     DAOMQG010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15580_length_2644_cov_7.863654"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="PLC06_15615"
     CDS             complement(<1..2038)
                     /locus_tag="PLC06_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008893098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRO91927.1"
                     /translation="MHDHHQHHSAEDGKPESAELMHHTADEAVHSGGHTHHAASQEAH
                     DSHDSHDSHGSHVDHTGHDDHSGHTDHTGHEAMFRRRFWVCLILTIPVLLYSGHVQML
                     LGFRPPQFPGSEWVAPVLSVIIFLYGGLPFLQMAAPEIRGRKPGMMTLISLAITVAFV
                     YSLVTVFVPIGADFFWELATLVTIMLLGHWLEMRSVRQASGALDELAKLLPDTAERVT
                     ADGRVEQVPAAQLREGDLFLVRPGAAIPADGEVAEGQTDVDEAMITGESRPVKKEPGS
                     QVIAGTVNSGDGSLRVRVTAVGEATALAGIMRLVRDAQQSKSSTQLLADRAAGWLFYV
                     ALAAAAITLIAWTAAIGFGSETIARVVTVLVIACPHALGLAVPLVVAITTSLSAHNGI
                     LVRDRSAMEAIREANVVIFDKTGTLTEGSHGVVGMAIVEGTDESAALALAAAVEGDSE
                     HFIAQAFRKAAEERGLSLPAIQRFEALKGRGVRAESGGEMVYVGGPRLLEMLSLPLPT
                     AIEAFTEETNRKGQSVIYLIRGEQVVAAFALADVIRPESQEAVRRLREMGVEVAMLTG
                     DSEAVAAAVAAELGIDRYFAEVLPETKDRQVAQLQAEGKRVVMVGDGVNDAPALTRAD
                     VGIAIGGGTDVAVESAGIVLVRSNPLDVVKTIELSRASYRKMIQNLWWAAGYNII"
     gene            complement(2131..2577)
                     /locus_tag="PLC06_15620"
     CDS             complement(2131..2577)
                     /locus_tag="PLC06_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRO91928.1"
                     /translation="MKRITLFFILFAGLPVLAACSVGGATESAPSPLVITLAATDIAY
                     DVDRIEANAGQTIHLTLDNQGALEHDFSIAEIPLSGETVVTEAAGEMAGHDMGHMANA
                     PTVHVAAPAGGQAIIEFTPDTPGEYVFNCTVSGHREAGMHGILIVN"
BASE COUNT          453 a          880 c          814 g          497 t
ORIGIN      
        1 cgatgatgtt gtacccggcc gcccaccaca ggttctggat catcttgcga tagctcgccc
       61 ggctcagctc gatggttttc accacgtcga ggggattgct gcgcaccagc acgatgcccg
      121 ccgattccac ggcaacatcc gtgccgccgc cgatagctat ccctacgtcg gcgcgagtca
      181 gcgccggagc gtcgttcaca ccgtcgccga ccatgaccac gcgcttgccc tcggcctgca
      241 attgggccac ctggcggtct ttcgtctccg gtagtacctc ggcgaaatag cggtcgatgc
      301 ccagttcggc ggccacggcc gcggccaccg cctcgctgtc gcccgtcagc atcgccacct
      361 ctacgcccat ctcgcgcagg cggcgcacag cttcctggct ctccggccgg atgacgtcgg
      421 ccagcgcgaa cgcggccacg acctgctcgc cgcgaatcag gtagatgacc gattgtcctt
      481 tgcggtttgt ctcctccgtg aaggcttcga tcgcggtcgg cagtggcagg gacagcattt
      541 ccagcaggcg cggcccgccg acgtagacca tctcgccgcc ggactcagcg cgcacgccgc
      601 gccctttcag tgcctcaaac ctctggattg caggtaacga cagccctcgc tcttcggccg
      661 ccttgcgaaa cgcctgggcg atgaaatgtt ccgaatcacc ctcgacggcc gccgccagcg
      721 ccagcgccgc cgattcatcc gtcccctcga cgatcgccat gccgaccaca ccatgcgacc
      781 cctccgtcag cgtgcctgtc ttgtcgaaga tgaccacgtt cgcctcgcgg atggcctcca
      841 tggccgaccg gtcgcgcacc aggatgccgt tatgagccga gagtgatgtg gtgatggcga
      901 cgaccagcgg cacggccaac cccagtgcat gggggcaagc gatgaccagc accgtgacga
      961 cccgcgctat cgtttccgag ccgaagccga tggccgccgt ccaggcgatc aaggtgatcg
     1021 ccgccgcggc cagggcgaca tagaacagcc atcccgcggc ccggtcggcc agcagttgcg
     1081 tgctcgattt gctttgttgt gcgtcccgca ccaggcgcat gatgcccgcc agcgcagtgg
     1141 cttcgcctac agccgtcacg cgcacccgca gactgccgtc gccgctgttg accgtcccgg
     1201 cgatgacttg gctgcccggc tccttcttca ccggtcggga ttcaccggtg atcatcgcct
     1261 cgtccacgtc cgtttggcct tcggccactt cgccgtcggc cggtatggcg gcgcccggcc
     1321 gcacgaggaa cagatcgccc tcgcgcagct gagctgccgg aacttgctcc acgcggccgt
     1381 cggccgtcac gcgttcggcc gtgtccggca acagcttggc cagttcatcc agcgcgccgc
     1441 tcgcctggcg cacgctgcgc atctccagcc agtggccgag gagcatgatc gtcaccagcg
     1501 tcgccagttc ccagaagaaa tccgcaccga tgggaacgaa gacggtgaca aggctgtaga
     1561 cgaaggccac ggtgatggcc agcgagatga gcgtcatcat ccccggtttg cggccgcgaa
     1621 tctccggggc ggccatttgc aggaagggca ggccgccata gaggaagatg atcacgctga
     1681 ggacgggagc aacccactcg ctgccgggga attggggcgg gcggaagccc agcaacatct
     1741 ggacgtgtcc ggagtagagc aggacgggaa tggtcaggat caggcagacc cagaagcgtc
     1801 gccggaacat ggcctcgtgg ccggtatggt cggtatggcc gctgtggtcg tcgtggccgg
     1861 tatggtcgac gtggctgcca tgactgtcat ggctatcgtg gctgtcgtgg gcttcctgtg
     1921 atgcggcatg gtgggtatgt ccgccgctat ggacggcctc atcggccgtg tggtgcatta
     1981 gttcggcgga ttcgggtttg ccgtcctcgg ccgaatgatg ctgatgatga tcgtgcatct
     2041 cgttctccat acggctcccc cgcatggcgc aagctgcaca atggttaatg accgatattc
     2101 acggacacca ctgtgtcgtt tgccactaaa ctaattgaca atcaggatgc cgtgcatccc
     2161 tgcttcccga tgcccggaaa cagtgcaatt gaacacatac tctcccggcg tgtcgggcgt
     2221 gaactcgatg atggcctgcc cgccggcagg ggcggcaacg tgaacagttg gtgcgttcgc
     2281 catatgcccc atatcatgac cggccatctc gcccgccgcc tcagtgacga ctgtttcgcc
     2341 ggagaggggg atttcggcga tgctgaagtc atgctccagc gcgccttgat tatccaacgt
     2401 cagatgtatt gtttgcccgg cgttggcctc gatgcgatcc acgtcgtaag caatgtccgt
     2461 ggcggcgagt gttatcacga gcggagaggg tgcgctttcg gtcgcgccgc ccaccgaaca
     2521 ggccgccaaa accggcagcc ccgcaaacag gatgaaaaac agggtaatcc gtttcatgga
     2581 tgagttttcc ttgacgagct attgaagctt caacggccgc tattgcgtcg tccgctccca
     2641 ctgg
//
LOCUS       DAOMQG010000437         2639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15615_length_2639_cov_8.368034, whole genome shotgun sequence.
ACCESSION   DAOMQG010000437 DAOMQG010000000
VERSION     DAOMQG010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15615_length_2639_cov_8.368034"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLC06_15625"
     CDS             <1..191
                     /locus_tag="PLC06_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91929.1"
                     /translation="AARLDAERAANLQQPFLRGPDGVACPAIRQSTAIRRHVLRVQRI
                     DCPGPGKNNYELRITNYE"
     gene            1075..1944
                     /locus_tag="PLC06_15630"
     CDS             1075..1944
                     /locus_tag="PLC06_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91930.1"
                     /translation="MVESLLETDLSRYKPDWERLGVVVAPFTIEADPRFMYLSTETRR
                     ALSKVSYMVEAGQGASVVVGEVGTGKTTLASWFHSRYDRRPDFVAARVDEPPKKSGLL
                     LLRRIAAEFGVRTRRATEDQLNELRDFLTETTARNVLPLVIIDEAHELEPEQFKELRR
                     LLNFRDPRGGKAYQLILLGRPELDINLRHSKDFNDRVATRSSLDPLAPDDTAALIEYR
                     LLAAGRDTRQPPLFLPDSVLPIWRETRGYPRSICLLCLHLCLELLATGESYVDEAFVM
                     KFLETHSGYRQAE"
     gene            2133..>2639
                     /locus_tag="PLC06_15635"
     CDS             2133..>2639
                     /locus_tag="PLC06_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91931.1"
                     /translation="MSKPKDPHGRNRDDEEPRVSTLKELLRDKPRRGRPRRQVARQNV
                     YVALHPEQKVQLDELAGLLPIDLGRADLPDLAVSVLTARVEALRTAVVGRTREIPEGV
                     TDLESLYLLWDVELPNREPADKWTSIRLSPQQVLEMGRVHGTLNAAFGATRSEVFGLA
                     LALLAQFLE"
BASE COUNT          523 a          858 c          752 g          506 t
ORIGIN      
        1 cggccgcgcg tctcgatgcc gagcgagcgg cgaatctcca gcaacctttc ttgcgcggcc
       61 cggacggcgt cgcttgtccc gcaatcaggc aaagcacggc catccgcagg catgtcctgc
      121 gtgttcaacg gatagattgc cccgggccag ggaagaacaa ttacgaatta cgaattacga
      181 attacgaatg aagaagtgcg caatagtcac tgatctactg atcactgacc tactgactac
      241 tgactactga ccactgatct actgacctac tgacctactg atctactgat cactgatcta
      301 ctgaccactg atcgctgcga ctcccgtcgc acgaccactt cttcactcgt atctcgtatt
      361 tcgtatctcg tatctcccat aattgacacg atcggccgca tatggtatgc tcgtacctgg
      421 atagagtagg cttcctttgg agacataaaa tccccctccc caatcaaggg gcgctgcttc
      481 ggatgatggc ccttgggcca tcgcccacgg tgttggcggc accgataagc ggaaaggagg
      541 ccgtcgctta tacgggtggc cctgcatggc ggggccactt ttttattgcc ggtctgccgg
      601 ccgattcatc gtcaggcaaa gctgaacaca cccgcggcgc ggcctgtttt ggtcaacgcc
      661 cggcgccgaa cggccgaccg gctattccct atgtcggcgc agaggccctc gcccactgcg
      721 cctggcgacc cgcggatggc ggtctttggg tatcgcctct cacttcacga cgatcatatc
      781 caccggaacc tcacttaatg agcgattcgt gatatattcg ccgacgatcc acagtataat
      841 agcacaatag ggctatgtca agatgatcta tagcatatag attgtaattc gtgatcgtgt
      901 acttggtcaa gacggccgaa tttacctgcc tcaggcctgt acaatcggct tgacatcctg
      961 tatacttcta tggatcatac ctgtagagca agctgtcagc ttgcctccgg gcaaagactc
     1021 aacctggcag gttgagctgc agtagattga tctacactct aacggagaaa cgcgatggtc
     1081 gagagccttc tggaaaccga tttgagccgc tacaaacccg attgggagcg gctgggcgtg
     1141 gttgtggccc cgttcaccat cgaagccgac ccgcgcttca tgtacctctc caccgagacc
     1201 cgccgcgcct tgtcgaaggt cagctacatg gtcgaggcgg ggcagggggc gtcggtggtc
     1261 gtgggcgagg tgggcacggg caagacgacg ctggcctcgt ggttccacag ccgctatgac
     1321 cgccggcccg acttcgtggc cgcgcgcgtc gatgagccgc ccaagaagag cggcctgctg
     1381 ctgctgcggc gcatcgccgc cgagttcggc gtgcgcaccc gccgcgccac cgaagaccag
     1441 ctcaacgagc tgcgcgactt tctgaccgag accaccgccc ggaatgtgct gccgctggtc
     1501 atcatcgacg aggcccacga gctggagccg gagcagttca aggagctgcg ccgcctgctc
     1561 aacttccgcg acccgcgcgg cggcaaggcg tatcagctca tcctgctcgg ccgccccgaa
     1621 ctggacatca atcttcgcca ctccaaagac ttcaacgacc gcgtggccac ccgatcctcg
     1681 ctcgaccccc tggcccccga cgacacggcc gcgctgatcg aatatcgcct gttggccgcc
     1741 ggccgcgaca cccgccagcc gccgctcttc ctgcccgact ccgtgctgcc catctggcgc
     1801 gagacgcgcg gctatccgcg ctccatctgc ctgctgtgcc tccatctctg cctcgaactc
     1861 ctggccaccg gcgaaagcta cgtcgatgag gcgttcgtca tgaaattcct ggagacccat
     1921 agcgggtatc ggcaagcgga gtgacgaacg aattacgaat tacgaattac gaattacgaa
     1981 ttcagaggtg acggccaaat gaacgactat cggttgcgcg acagggcaag gcgccggcta
     2041 tgaggatttc ctgccccgaa gatcatacga aggagcgaac tctcttcatt cgtaattcgt
     2101 aattcgtaat tcgtaatttg taatctgtaa ctatgagcaa gcccaaagac ccccacggcc
     2161 ggaaccgcga cgacgaagag ccgcgcgtca gcacgctgaa ggagctgctg cgcgacaagc
     2221 cacgccgcgg ccgcccgcgc cgccaggtgg cccgccaaaa cgtctatgtg gcgctgcatc
     2281 cggagcagaa ggtccagctc gacgaactgg ccggcttgct gcccatcgac ctcggccgcg
     2341 ccgacttgcc cgacctggcc gtctccgtgc tgaccgcgcg cgtggaggca ttgcgcacgg
     2401 ccgtcgtcgg ccgcacgcgc gagatccctg aaggagtcac cgacctcgaa tcgctctacc
     2461 tgttgtggga cgtggagctg cccaaccgcg agccggccga caaatggacg agcatccgcc
     2521 tctcgccgca gcaggtgctg gagatggggc gggtgcatgg cacgctcaac gccgcgttcg
     2581 gcgccacccg cagcgaggtg ttcggcctgg ccctggcgct gctggcccag ttcctggaa
//
LOCUS       DAOMQG010000438         2629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15675_length_2629_cov_8.908702, whole genome shotgun sequence.
ACCESSION   DAOMQG010000438 DAOMQG010000000
VERSION     DAOMQG010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15675_length_2629_cov_8.908702"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..98
                     /locus_tag="PLC06_15640"
     tRNA            27..98
                     /locus_tag="PLC06_15640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Lys,seq:ctt)
     gene            complement(144..>2629)
                     /locus_tag="PLC06_15645"
     CDS             complement(144..>2629)
                     /locus_tag="PLC06_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO91932.1"
                     /translation="VLGELPRLFFPVTEGVGGIPVPMIGREKEMTALKSLLVGAARDG
                     RGGVITLVGDAGVGKSRLAREFHRQLDEFPFKLFVLQARTDQRLAGVPFSLMRDLLAR
                     CFNIEDGDDANRIEEKIMNGLRISLENDGSTGQRSHLRERARAIGLLVGLDLPSRHPE
                     PWERGGVGAIRERAIEAILEFLDAAIHNAPATVMVLEDIHWADEDSLALLERVAAGAA
                     DRPLLMLCLTRPTFLEQRPRWPGEEPVGALSLPVRPLDDRGSRDLVLSILRRLPDSPP
                     SLVDLIIRSAAGNPYYVEELVRVLIEDGIIVTGQDDWYFRPRELIRMRVPATLTGVLQ
                     ARLDRLPDPERAILQQAAVIGDEFWTGAVQSLNQAARFPYPEERIIQTLNSLEQRDMI
                     VRSASAASVGSGSYLFRHTLLREVAYESVLLRDRPGYHLQAARWLESQRSDRSEDYAA
                     PVARHYEQAGRPAEAARMFMQAAARATDQFKLPVAIDFYRRALDLLRSLPQHLDEQLE
                     ALERLGRLLWRRGRLIEARDIYRELHDSAGLDGNLLIQARAGNELATVALEMGDARQA
                     MVEATGAEQSARLTGADIEQIDALILQAKAAGQSGVRKSAAEAAHRALELASSLYAPR
                     RFAHASSLTTQYTDDTAKRNTALKEMRSLAEALAGQGQAEDAAYAFGRLGEGLLALGR
                     PGDAREALAHALVAQHDGGDRGELVELLRLDGLAACRLGDAGVALSLLEKAESLADES
                     GDRYLRLACRLAAGEVLLARGELAAAEATLRQVITATENRQRLGNWAQLISARTLLVE
                     ALNRQGRTDEARLAGFATSHQRTFTDSPP"
BASE COUNT          512 a          871 c          745 g          501 t
ORIGIN      
        1 catggtaaaa tactcgaagc ttgagaggcc ctgtagctca acggcagagc agttgactct
       61 taatcaattg gttagaagtt cgaatcttct cagggtcaca accaacgatc gaatcggcgt
      121 ccatttcggc gccgattttt cgtttatggg ggactatcgg tgaaagttcg ttgatgactt
      181 gtcgcaaaac cggccagcct ggcctcatcc gtccgcccct gacgatttag cgcctcaacc
      241 agaagggtgc gagccgagat taattgcgcc cagttgccca gtcgctgccg attttcggtt
      301 gcggtgatga cctggcgcag ggtggcctcg gctgccgcaa gctccccgcg agccaacaac
      361 acctcgcccg ccgccagccg gcaggccagc cgcagatagc gatcgccact ctcatcggcc
      421 aggctttcgg ctttttccag cagcgacagc gccacccctg catcgcccag ccggcaggcc
      481 gccaggccat ccaaccgcaa tagctccacc agttctcccc ggtcgcctcc atcatgttgg
      541 gcgacaagag catgggccaa cgcttctcgc gcgtcgcccg gccgtcctag tgccagcaac
      601 ccttctccca atcggccgaa tgcgtaagcg gcgtcttctg cctggccttg tccggccaac
      661 gcctcagcca gcgagcgcat ttccttgaga gccgtattcc gctttgctgt gtcgtcggtg
      721 tattgtgttg tcagcgatga agcatgcgcg aaacgccgag gcgcgtatag cgagcttgcc
      781 aattccaacg ctcggtgcgc agcttcggcc gccgatttcc gcacccctga ctgcccggcc
      841 gccttggctt gcaggatcag cgcgtcaatc tgctcgatgt ctgccccggt caggcgcgcc
      901 gactgctctg cccccgttgc ctcgaccatc gcttgtcggg catcccccat ttccagcgct
      961 acggttgcca attcattgcc ggcccgtgcc tgaatcagaa gattgccgtc cagcccggcg
     1021 ctatcgtgca gctcccgata gatgtcgcgg gcctcaatca gacggccgcg tcgccataaa
     1081 agtcggccca gccgctccag ggcctccagc tgctcatcaa ggtgttgggg caaactacgc
     1141 aacaaatcga gcgctctgcg ataaaaatcg atagcgacgg gcagcttgaa ctgatcggtg
     1201 gcgcgggcgg ccgcttgcat aaacattcgc gctgcttccg ccggacgacc ggcctgctca
     1261 taatgccggg cgacaggtgc ggcataatcc tctgaccgat cgcttcgctg cgattcgagc
     1321 cagcgcgctg cttgcaaatg atagcccggc cggtcgcgca gcagtacgct ctcataggcc
     1381 acttcgcgca acagcgtgtg ccggaagaga tagcttccac tcccgacaga agcagccgat
     1441 gcagaccgca caatcatgtc gcgttgttcc agcgagttga gggtctgaat aatccgttct
     1501 tcagggtagg ggaatctggc tgcctgattc agcgattgga cagcacccgt ccaaaattca
     1561 tcgccgatta cggccgcctg ttgcaggatc gcgcgctccg ggtccggcaa acgatccaga
     1621 cgcgcctgta gcactccggt gagagtggcg ggcacgcgca tccggattaa ctcgcgcggg
     1681 cggaaatacc aatcgtcctg cccggtgacg ataatgccgt cttcgattag cacccgcacc
     1741 aattcttcga cgtagtacgg attcccggca gccgaacgaa tgatgaggtc gaccaggctc
     1801 ggcggggaat cgggcaatct gcgcagaatg ctcagaacca gatcgcgact gcctcgatca
     1861 tcaagcggcc gaacgggcaa ggatagcgcg ccgacgggtt cttcacccgg ccaacgcggt
     1921 cgctgttcca gaaaagtagg ccgcgtcagg cacaacatga gcagcgggcg gtcggcggcg
     1981 cccgccgcca cccgttccag caaggccagc gaatcctcat ccgcccagtg gatatcctcg
     2041 aggaccatga ccgtggccgg cgcattgtgg atcgcggcat caagaaattc gagaatcgcc
     2101 tcaatcgccc gttctctgat cgcgccgacg cctccgcgct cccacggctc cgggtggcga
     2161 gacggcaagt ccagcccgac gagcagccct atggcccgtg ctcgctcgcg caggtgtgat
     2221 ctttgccccg tacttccgtc attctccagc gagatgcgaa ggccattcat tattttctct
     2281 tcgatacgat tcgcatcgtc cccatcctcg atgttgaaac atcgggccag caagtcccgc
     2341 atcaggctga atggcacacc ggccaatcgt tgatcggttc gcgcctgaag gacgaaaagc
     2401 ttaaacggga actcatccag ttgccggtga aattcgcggg cgagacgaga tttaccgacg
     2461 ccggcatcgc cgacaagtgt gatgacccct cctcgcccgt cgcgggcagc gccgaccagc
     2521 aacgacttca gcgccgtcat ctccttctcg cggccaatca tcggcacggg gatgccgcct
     2581 acgccctcgg tgacgggaaa gaagagacgc ggcaactcgc cgagcactc
//
LOCUS       DAOMQG010000439         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15729_length_2622_cov_10.802493, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000439 DAOMQG010000000
VERSION     DAOMQG010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15729_length_2622_cov_10.802493"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLC06_15650"
     CDS             complement(<1..778)
                     /locus_tag="PLC06_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRO91933.1"
                     /translation="MNPQTRLWLIVGVTLIYFALMVGSMVGDSPTMDEQNHIARGLAF
                     LRTGDPRLSLEHPPLINSLSALPLLTMPELRLPTDHPSWERREGWYEFADLFMWQYNH
                     DVTRIVFLARLPIIFLAIALALAGLRFARSLWGDAAALPAFVLLLFEPNLMAHGRYAT
                     TDMGGILFTFLATYALWRLWQYPEGWSWRRWLTGAILMGLAFSSKTSALGFVVIWFVL
                     ALLPLYPSGFGRRGRQALIRVAQLLSAGALSLVVVWAIFGF"
     gene            857..1936
                     /locus_tag="PLC06_15655"
     CDS             857..1936
                     /locus_tag="PLC06_15655"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRO91934.1"
                     /translation="MSAIYEASDVSSRIIYNLTPMAPHSSSPETTSKGPSARKRLLTI
                     LKISITIVGLILVINGIDFQVLLTYISEVKWSWMTVGAGLIGVSLVIRSYRWHILLSG
                     VGSKIRFSRLLSLYLVGSFFNAFLPSGLGGDVVRAAEAAQDVEPSMAVGTVLVDRLTG
                     LMALFMMALAVLPFRPPEFPGYLVITIAGICTLGLIFGFILVDGRLFMWFIHHLPGAI
                     RQLGDGFIGRLASSIQNCGGRTLTKALLVSVLFNLVQVGWWAAAGHALQLSIPYTYYL
                     LVVPIMSLALLVPSIGGLGVREFLSPTLFRGAGLSPEQSVALTLLVFGLERVTSLLGA
                     PVYLYSTFRAHREDQTTDSARQNNP"
     gene            complement(1996..>2622)
                     /locus_tag="PLC06_15660"
     CDS             complement(1996..>2622)
                     /locus_tag="PLC06_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HRO91935.1"
                     /translation="AATAELRATLSDPSVSSYRVVLQPEKMVMREAERAVSYLGLFNY
                     PVDSVIINRILPPSSGDSEFYRERRAIQAKYIELIENNFRPLPIWYAPYYSHEVVGFD
                     ALDKLASDCFGDADPGDIFYRGIVQEIVDSGDGSYLLRLPIPFIDKDDVKLRKRGDEL
                     FITIGNFKREMILPAVLAKRRAGGGRLRDGFLEISFLPEEGEAKATPA"
BASE COUNT          592 a          743 c          705 g          582 t
ORIGIN      
        1 cgaagccaaa gatagcccac acgaccacca gagataacgc gccggccgat agcagctggg
       61 ccactctaat cagggcttgt cgacctcgac ggccgaagcc gcttgggtac agcggcaata
      121 aggccagcac aaaccagatg accacaaacc ctaaagccga tgttttgctg ctaaaggcca
      181 gccccatcag gatagcgccg gtcaaccatc gtctccagct ccatccttca ggatattgcc
      241 acagccgcca aagcgcatac gtcgccaaaa aagtaaagag aatgccgccc atgtcggtgg
      301 tggcataccg gccgtgggcc atcagattgg gttcaaagag gagcaacaca aaggccggca
      361 acgcggccgc atcaccccac agcgaacggg caaatcgaag cccggccagg gcaagagcaa
      421 tagcgagaaa aataattggc agtcgagcga gaaatacgat acgggttaca tcatggttat
      481 attgccacat gaacaggtcc gcgaactcat accacccttc gcgtctttcc caactggggt
      541 ggtctgtcgg caggcgcagc tccggcatcg tgagcagcgg taaggcgctc aggctgttaa
      601 tcaatggcgg atgctccaga cttaaccgcg gatcacccgt cctgagaaat gccagaccgc
      661 gagcaatatg attctgctca tccatcgtcg ggctatcgcc aaccatgcta cccaccatca
      721 gggcaaagta aatcagcgtg acgccaacta tcagccacag acgagtctgc gggttcattc
      781 gcaagtggat catgcttggt gcgggcctat cggacataac tgaggcgatt ataaactatt
      841 gaggcccagg agggttatgt cggcaatata cgaagcgagt gacgtttcct cacggatcat
      901 ctacaatctc actccgatgg ctccccattc atcatccccc gaaacgactt cgaaaggacc
      961 ttccgctcgc aagcgtctcc tgacgattct caagatatca atcacgatag ttggcctcat
     1021 ccttgtaatc aatggaattg atttccaggt tctgctgacc tatataagcg aagtcaagtg
     1081 gtcctggatg acagtcgggg caggactgat cggggtcagc ctggtcattc gatcctatcg
     1141 ttggcatatc ttgctcagcg gggtcggctc gaaaattcgg tttagtcgcc tgctgtctct
     1201 ctacctagtg ggcagctttt tcaacgcctt tctgccgtcg ggattgggtg gtgatgtagt
     1261 ccgggcggcc gaagccgcgc aggatgtgga acccagcatg gctgtcggca cagttcttgt
     1321 cgaccgcctg accgggttga tggccctgtt catgatggcc ctggcagtcc tgccatttcg
     1381 cccgccggag tttcccggct atctggtgat tacaatcgca ggaatatgca ctttgggcct
     1441 gatttttggg ttcattctcg tcgatgggcg gttgtttatg tggttcatcc atcatctgcc
     1501 gggagcgatt cggcaactgg gcgacggctt tattggcagg ctggcatcat cgatccaaaa
     1561 ttgcggtggg cgcacattga ccaaggccct gctggtatcg gtgttgttta atctcgttca
     1621 ggtaggctgg tgggcggccg ccgggcacgc cctgcagtta tcgatcccct atacctacta
     1681 tcttcttgtg gtgccaatca tgtcgctggc gcttctggtc ccgtcaatcg gtggccttgg
     1741 cgtgcgagaa ttcctgtcgc cgaccctctt tcgtggtgcg ggtctatcgc cggaacaatc
     1801 tgttgcgttg acgcttctgg tattcgggct ggaaagagtg accagcctgc tcggcgctcc
     1861 cgtctatctt tactcaacct ttcgcgctca tcgggaagac caaaccacag atagcgcacg
     1921 tcaaaacaac ccataaaata acgccgggac atcttataag tcccggcgtt gttcagacat
     1981 tggagcaatt ttcgcctatg cgggtgtggc ttttgcttcc ccttcttcag gcaaaaagga
     2041 aatctccagg aatccatccc gaagacggcc gccgccggcc cgtcgtttgg ccaggacggc
     2101 cgggaggatc atctcccgtt tgaagttgcc gatcgtgata aagagttcat ctccacgctt
     2161 tcgcagcttg acgtcatcct tgtcgatgaa cgggatcgga agacgcagca ggtaactgcc
     2221 gtctccggaa tcaacgattt cctgcacgat cccgcgatag aaaatatcac caggatcagc
     2281 gtcaccgaag caatcggacg ccaatttatc cagcgcatcg aacccaacga cctcatgcga
     2341 gtaatacggt gcgtaccaaa taggcagagg gcggaagtta ttctcgatca gttcgatata
     2401 tttggcctga atcgcccggc gctcgcgata aaattcgcta tctccggagg atggcggcaa
     2461 gatgcgattg atgatcacgc tatccaccgg atagttgaac aggcccagat aactgaccgc
     2521 tcgctcggcc tcgcgcataa ccatcttttc gggttgcaac acgacacggt agctgctcac
     2581 ggatggatcg ctcaacgtag cgcgcaactc ggccgtggcg gc
//
LOCUS       DAOMQG010000440         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15738_length_2621_cov_9.086126, whole genome shotgun sequence.
ACCESSION   DAOMQG010000440 DAOMQG010000000
VERSION     DAOMQG010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15738_length_2621_cov_9.086126"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1216)
                     /locus_tag="PLC06_15665"
     CDS             complement(92..1216)
                     /locus_tag="PLC06_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO91936.1"
                     /translation="MIYVDISAAVHSKAGLGRYSERLAGALLARDPGRYALFYNRGGA
                     GHFPESLPANAPRREVRWGYKPWRMAILMGQYGRVPFNRLVPDAELFHSTEHLLLPLR
                     NIPTVLTVHDLIYKLFPDYHKRLNYWYLNRAMPLYVERASAIIAVSEATKRDLVKFYG
                     VDPAKVTVVYEAAASHFRPPTPARIDYVRQTYALPERFLLHLSTIEPRKNLMRLLDAF
                     HILRNSNPDLHLLLAGSRGWLFDEFFARIEAEGLQDIVRPLGWVPDEDLPAVIGAAAL
                     AVQPSLYEGFGLPILEHMACAQVVAASNSSSHPEVGGEAAAYFNPTDVEEMAAVIGRL
                     LIDRDEYRRRQALGLEQAARFSWERAATETAAVYDRLLAK"
     gene            complement(1249..2061)
                     /locus_tag="PLC06_15670"
     CDS             complement(1249..2061)
                     /locus_tag="PLC06_15670"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRO91937.1"
                     /translation="MPQKPTAYYRPTNLDEALRYLRQPNTLPLAGGTALLATEDGITA
                     AVVDLQNTGLDHLTWADDGRLLRIGAMVRLADLDEFLAPLAALEGAAALVRNAIHRAG
                     PNTYRNAATVGGIIASRLPDSELLAALLVLDATVSLRLPAPETVSLATYLRDEERLPG
                     LITDIMIFWPAGKSASERVARTPADYPIVSVTGWRPASGAIRLAATGIGPHPIHLVTA
                     EVALGGGLNEDAIATAAQAAAKSVDHPGDFRGDAAYRAEMAAVLTRRVLAAL"
     gene            2450..>2621
                     /locus_tag="PLC06_15675"
     CDS             2450..>2621
                     /locus_tag="PLC06_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRO91938.1"
                     /translation="MLEDIARLEADTERDTATNVEAILEPLARVFIHNDDVTPYDFVV
                     LVLQRFFGLNPLE"
BASE COUNT          487 a          781 c          868 g          485 t
ORIGIN      
        1 agatgagcag cgtgacgacg agatatttcc tgatcatgcg tttgagtatt atccaaaatt
       61 taaggcctgg gcggtagcct taaccgcgcg ctcacttggc gagaagccga tcgtagacgg
      121 cggccgtctc ggtcgcggcg cgttcccagc tgaagcgggc cgcctgctcc agccccaggg
      181 cttgccgccg gcggtattcg tcccggtcga tcagcaggcg gccgatgact gcggccatct
      241 cctcgacatc ggtcggatta aaataggctg ccgcttcgcc gccgacctcg gggtggctgc
      301 tgctgttgct ggcggcgacg acctgagcgc aggccatatg ctccagaatg ggcaggccga
      361 acccctcata gagactgggt tgcacggcca gcgccgccgc gccgatcacc gccggcaaat
      421 cttcgtcggg gacccagcct agcggccgta ctatgtcctg caagccctcg gcctcgattc
      481 gcgcgaagaa ctcgtcgaac agccagcccc ggctcccggc caggaggagg tgcagatcag
      541 gatttgagtt gcggaggata tggaaggcgt cgagcaggcg catgaggttc ttgcgcggct
      601 cgatggtgct caagtgcagg aggaaccgct cgggcaaggc gtaagtctgg cggacgtaat
      661 caatcctggc cggggtcgga ggtctgaaat gggatgcggc ggcctcatac acaaccgtca
      721 ctttagccgg atcgacgccg tagaacttga cgagatcgcg tttggttgcc tccgacacgg
      781 cgatgatcgc cgacgctcgc tcgacgtaga gcggcatggc gcgattgaga taccagtagt
      841 tgagccgctt gtggtagtcg ggaaagagct tgtagattag atcgtggacc gtcagcaccg
      901 tgggtatgtt gcgcaagggc agcagcagat gctcggtgct gtggaacaac tcggcgtcgg
      961 gcaccaggcg attgaacggc acgcggccat attgccccat gagaatggcc attcgccacg
     1021 gtttgtagcc ccaacgcacc tcgcgccgcg gggcgttggc cggtagcgat tcggggaaat
     1081 gaccggctcc gccccggtta tagaacaggg cgtagcgccc cggatcacgc gccagcaaag
     1141 ctcccgccag ccgctcgcta tagcggccga gaccggcttt actatggacg gcggccgaga
     1201 tgtcgacgta gatcatcggt agcgcagggg cgttctgccg gaacgcctct acagagcagc
     1261 cagcacccgc cgggtcaaca cggcggccat ttcggcgcgg taggcggcat cgccgcgaaa
     1321 atcgccggga tggtcgacag acttcgcggc ggcctgtgcg gcggtggcga tagcgtcctc
     1381 attcagaccg ccgcccaggg cgacttcggc cgtgaccaaa tggatggggt gcgggccaat
     1441 gccggtggcc gccagccgga ttgcgccgct cgccggtcgc cagccggtga cggagacgat
     1501 ggggtagtcg gccggcgtgc gggccacgcg ctcggacgcg ctcttgcccg cgggccagaa
     1561 gatcatgatg tcggtgatga ggcccggcaa ccgctcctcg tcacgcagat aggtggccag
     1621 cgataccgtt tccggcgcgg ggaggcgcag actgacggtg gcatcgagga cgagcagggc
     1681 ggccagcaat tcgctgtccg gcaggcggga ggcgatgatg ccgcccacgg tggctgcgtt
     1741 gcgataggta ttggggccgg cgcggtggat ggcgttgcgc acgagcgcgg ccgcgccctc
     1801 cagcgccgcc agcggggcca ggaactcatc caggtcggcc aaccgaacca tcgcaccgat
     1861 gcgcaagagg cggccgtcgt cggcccaggt caggtggtcc agccccgtat tttgcaggtc
     1921 caccacggcg gcggtgatgc cgtcctcggt ggcaagcaag gctgtgccgc ccgccagcgg
     1981 cagggtattc ggttggcgca ggtagcgcaa ggcctcatcc agattggtgg gtcgataata
     2041 ggctgtcggt ttttgtggca tggcgataga tccccataag gtaaaggcaa atatatctgt
     2101 catcttagcg cgggaaccca tggtgcgcaa gccggggatt ggttcgcctg catcctggag
     2161 ttaatcgcca ccacgatcat ggattccgtt caggcgcttg ccgatatggg cgcagcaaat
     2221 tcatccaatt ttgtaagggt gtcttggctc ttgacgcata gccaggtcaa tcttatttag
     2281 gacgggtcac gcgcttcttt cgtacttcgt cctttcctga tcagtgtacg ggcgggacaa
     2341 ccggcgcgat accccatcga atcaccagca gttcgctcgc cggttgtctc gcccccactt
     2401 tgaacatcgc ccaccatcga gtaaaatcag gcgttcaggg tgatggcaaa tgcttgagga
     2461 tatcgcgcgg ctggaagccg acaccgaacg ggacaccgct accaacgtcg aggcgatcct
     2521 cgagccattg gccagagtct tcattcataa tgatgacgtc actccttacg acttcgtcgt
     2581 gctcgtttta caacgatttt tcggattgaa ccccctggaa g
//
LOCUS       DAOMQG010000441         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15772_length_2618_cov_10.572766, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000441 DAOMQG010000000
VERSION     DAOMQG010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15772_length_2618_cov_10.572766"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLC06_15680"
     CDS             <1..830
                     /locus_tag="PLC06_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRO91939.1"
                     /translation="SLGMAGARRILELINTETDLDENPEGYAQPIIGEVRFEHVTFGY
                     VPGVDTLTDISFTVQPGQTVAIVGQTGAGKSTLTKLINRTYDVHEGRVLIDGVDVRDW
                     SLASLRRQISIIEQDIFLFSRTVAENIAFGAPGATQEEIEAAARAAQAHDFIMRFESG
                     YETVVGGRGVTLSGGQRQRIALARAFLTNPHILILDDSTSAVDSETEDQIQRAIRHAS
                     ENRTTFLITHRLSQIRWADLVLVLRGGRLEIAGDHTALMETSPAYRQIFDQYDIKEG"
     gene            complement(1074..>2618)
                     /locus_tag="PLC06_15685"
     CDS             complement(1074..>2618)
                     /locus_tag="PLC06_15685"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRO91940.1"
                     /translation="RRRLEEMGLKVAVTGRPKHIYSIYRKMKRKELDFSQIYDIQALR
                     VIIEPSDSDLSSEKRQGTKLRKERDDLERDLCYQVLGAVHSLWQPIRGEFDDYIGSPK
                     ANGYKSLHTAVIDSESGQKLEVQIRTVRMHEEAERGIAAHWAYKEQNTKVSTSAQRQI
                     QNLRDLLVSLQEDEEEGEGIVDVGKPEERIHVYSPTGQVIDLPAGSTPIDYAYQIHTG
                     LGHICRGAKVNDKLVGLDYQLQSGDKVEIVKGNRGGPNRDWMNPSLGYTGSARTRSKI
                     RQWFRQQEREQNIAQGREVIQRELKRLGLTDVFTVEDIGRALKFDDIDEFLSKVGFGD
                     IQTAKITGAIALMQQALRVEDEELLPLFKPPSPKRKGLTVRGVEGLYTRFAGCCNPIP
                     PDPIIGYITRGQGVTIHSQACDQLKGIPDKERLIEVDWGAGDERYPIPIVVTAYRRGS
                     LIEDLVSTLRGQKINVPRTKLVTSDNIMTVYLIAEVTDIQQLNWLLAKLSNLPKIIKV
                     QRQHWA"
BASE COUNT          502 a          710 c          767 g          639 t
ORIGIN      
        1 tatctctggg catggccggc gcccgtcgta tcctggagct cattaacacc gagacagatc
       61 ttgatgagaa tccggaagga tatgcgcaac ctattatcgg cgaagtgcgg ttcgagcacg
      121 tgacatttgg ctatgtcccc ggcgtggata ccttgacgga tatttctttt actgttcagc
      181 ccggtcagac tgtcgccatc gttggccaga cgggggcggg caagagcacg ctgacaaagc
      241 tcatcaatcg tacctatgat gtccatgaag ggcgggtgct catcgatggc gtggatgtgc
      301 gtgactggag cctggcatcc ctgcggcggc aaatttctat catcgagcaa gatattttcc
      361 tgttctcgcg aacggtggcc gagaacatcg cctttggcgc gcccggggct acccaggagg
      421 agattgaagc cgcggcgcgc gccgcgcagg ctcatgactt catcatgagg tttgaaagcg
      481 gctatgagac cgttgtcggt ggtcgagggg ttactctatc cggtggccaa cgtcagcgta
      541 tcgccctggc gcgcgcgttc ctcaccaatc cgcacatact gattcttgac gactccacga
      601 gcgccgtgga cagcgagact gaggatcaga ttcagcgggc catccggcat gcttccgaga
      661 accgcaccac tttcctgatc acccatcgcc tctcgcagat acgatgggcc gacctggtcc
      721 tggtattgcg cggcggccgt ctggaaattg cgggcgacca tacggccttg atggaaacat
      781 cccctgctta tcggcaaatc ttcgaccaat acgatattaa ggagggctag agttggtcat
      841 ggggccgcta gtctattatt gccgctggca gcgtgccaga ttacagcttc aggggcggga
      901 tggacaatgg gtctggggct ttcttgtctt tgaggacgat aacccccaaa ggagtgagcc
      961 atttcggtat gatagccggg caaggcagct gtatctgggg gaggggggag atcggtcgcc
     1021 gcttcagttg gatgagatgg gcgttgtcat tcgggacaac gcgggctagt cgcctaggcc
     1081 cagtgctggc gttggacttt gatgatcttg ggcaggttgc tcagcttggc gagtagccag
     1141 ttcaactgct gaatatccgt cacttcggcg atcagataaa cggtcatgat attatccgac
     1201 gtgaccaatt tcgttctggg cacgtttatt ttttgccccc gcagcgtgga gaccaaatcc
     1261 tcaatcagag agcctcggcg ataggcggtg acgacgatgg ggattggata acgttcatcg
     1321 cctgctcccc aatccacttc aatgagtctt tccttatccg gaataccctt gagttggtcg
     1381 caggcctggg aatggatcgt caccccttgc ccacgggtga tatagccgat gatcggatcg
     1441 ggggggatgg gattgcagca accggcgaag cgagtgtaga gtccctcgac cccgcgcaca
     1501 gtcaagccct tgcgtttggg tgaaggtggc ttgaataatg gcaatagctc ttcgtcttcg
     1561 acccgcaaag cctgctgcat aagggcgatg gcccccgtga ttttcgccgt ttgaatatcg
     1621 ccaaagccga ccttggaaag gaactcatca atatcatcga actttagcgc tcggccgatg
     1681 tcctcaaccg tgaagacgtc ggtcaggccc aatcgtttca gctctcgctg aataacttcc
     1741 cggccctgcg caatgttttg ctcgcgctcc tgctggcgga accattggcg gatcttgctg
     1801 cgcgttcgtg cgctgcctgt atatccgagg ctggggttca tccagtcgcg gtttgggccg
     1861 ccgcggttgc ctttgacgat ctctactttg tctccggatt gcagttggta atccaggccg
     1921 acaagcttgt cgttgacttt tgctcctcga cagatgtgtc ccaacccggt gtggatttga
     1981 taggcatagt caatgggcgt tgagccggcg ggcaggtcga tcacttgtcc ggtcggggaa
     2041 tagacgtgga tacgctcctc aggcttgccg acgtccacga tcccctcacc ttcttcctcg
     2101 tcctcctgaa gagatacgag caggtcgcgg agattctgga tttggcgctg ggccgacgtg
     2161 ctgacctttg tattctgttc cttgtaggcc caatgagccg cgataccgcg ttcggcctct
     2221 tcgtgcatac gaactgtgcg gatttgcact tccagctttt gaccggattc ggaatcaatt
     2281 acggccgtat gcaacgactt gtagccgttt gctttgggcg agccgatata atcatcaaac
     2341 tcgccgcgga ttggttgcca taggctatgg acggcgccca gaacctggta gcaaaggtct
     2401 cgctccagat cgtcccgttc tttccgcagt ttggtgccct ggcgcttttc gctgcttagg
     2461 tctgaatcag acggttcgat gatgacgcgc aaggcctgga tgtcgtagat ttgactgaaa
     2521 tccagctcct tgcgtttcat tttccgatag atggaataaa tgtgcttcgg ccgcccggtg
     2581 acggccactt taaggcccat ttcctccagt cgccggcg
//
LOCUS       DAOMQG010000442         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15961_length_2595_cov_10.400787, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000442 DAOMQG010000000
VERSION     DAOMQG010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15961_length_2595_cov_10.400787"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1835)
                     /locus_tag="PLC06_15690"
     CDS             complement(51..1835)
                     /locus_tag="PLC06_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRO91941.1"
                     /translation="MGASPFEFVDMNPEEEVHLTELRPTVQARFPDGRVFEGHRGTPI
                     EEFILAADLPKGERVVASLMNGRLRELCFPLQEDADLVPVTTATNDGARIYRRSLSFL
                     MIVAAAEVLPGQVITIFHSMPFGGYYCERDGGQRLTDEELAALRRRMRELVEADLPID
                     NVRLPLDEALAFFRKQGDQEKADLFARRRKDYLTLYELNGVRDYFHGFMVNRTRYLDL
                     FDLRHYNDGFILQFPRRSWPNVLQPFEDEPRLAQIFQTYKDWLTIMGVGNVTALNEAI
                     ANGRSREIILVAEALHERQLASIARSIANRQPSTRLVAVSGPTSAGKTTFAKRLALQV
                     MAQGIYPVIISLDDYFVNREDTPRDAFGHHDYEALEAVDVALFQDHMRRLMADEEVIM
                     PRFNFKTGMREEGSPLHIGREHVILVEGIHGLNPRLTDNLPPSATFRVFISAFTQLNL
                     DKHNRVPTTDTRLLRRIVRDASHRGYTAAQTIGRWSSVRRGEKNRIFPYQNTADVFFN
                     SALVYELSALKPLAEPLLLQVEAGTMERLEANRLMAFLQWFEPMSIPDLQFIANDSIL
                     REFIGGSVLETFQPSHWYATERGRQRIS"
     gene            complement(1988..2470)
                     /locus_tag="PLC06_15695"
     CDS             complement(1988..2470)
                     /locus_tag="PLC06_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91942.1"
                     /translation="MIVKEAVRGELVPFGDGLALRFDSRDDAETVYLTYALVTQGPEL
                     HVLPAMLLDDWGTEIGGLRLYRWVEENGLRFPRAEVFGQDPNGNTVQYFVRDLELFGQ
                     YPLYAFSADDAPVTSGVLLDAVIIPREGIALPEPVDLPDGISAPLRRAKVGWWAVAKE
                     "
BASE COUNT          623 a          758 c          687 g          527 t
ORIGIN      
        1 ccgccttttc ccaaatacaa gataatccgc attacaaggt ttctccttca tcaggatata
       61 cgttggcggc cgcgttcggt tgcgtaccaa tgacttggct ggaaggtttc gagaacagaa
      121 ccgccaatga attcccgcag aatcgaatcg ttggcgatga attgcaaatc cgggatcgac
      181 atcggctcaa accattgcag gaatgccatc aaccggttgg cctccagtcg ctccatagtc
      241 cccgcctcca cctgcaaaag gagaggctcc gccagcggct tgagcgccga aagctcatag
      301 acaagcgccg aattaaaaaa tacatcggcg gtgttctggt agggaaagat acggttcttc
      361 tcgccgcgcc gaacactgct ccaacggccg atcgtttgcg cggccgtata tccacgatga
      421 gaggcatcgc gcacgattcg ccgtaaaagg cgcgtatctg tcgttggaac ccggttatgc
      481 ttgtcgaggt ttagctgcgt gaaagccgag ataaagacgc ggaatgttgc cgatggaggc
      541 aaattgtccg tcaaacgcgg gttcaggcca tggatacctt ccaccaggat cacatgttcc
      601 cggccgatat gcaatggaga gccttcctca cgcatgccgg tcttgaaatt aaatcgcggc
      661 atgatcacct cttcatcagc catcaatcgc cgcatatggt cctgaaacaa ggcgacatcg
      721 accgcttcca gagcctcata atcgtgatgg ccgaaggcat cgcggggggt atcttcgcga
      781 ttcacaaagt aatcatcgag gctgatgatg accggataaa tcccttgagc catcacctgt
      841 agggcaaggc gcttggcaaa cgtcgttttt ccggccgatg ttggccccga aacggcgacg
      901 aggcgcgtcg atggctggcg attggcaata gagcgagcga tactcgccag ctgccgctca
      961 tgcaaagctt cagcaacgag gataatctcc cggctccgtc cgttagcaat ggcctcattg
     1021 agcgcggtca cattcccgac acccatgatc gtcagccagt ctttatacgt ctggaagatc
     1081 tgcgccagcc gtggctcgtc ctcaaagggc tggaggacat tgggccagct ccgccgggga
     1141 aactggagaa taaaaccgtc gttataatgg cgcaggtcaa acaggtccag atagcgagtt
     1201 ctgttgacca tgaagccatg gaaataatcg cgcaccccat tcagctcgta gagcgtcagg
     1261 tagtccttgc ggcgacgggc aaagaggtcg gccttctcct gatcgccctg ctttcggaag
     1321 aaggccagtg cttcgtcgag gggcaggcgc acattgtcga taggcaaatc agcctcaacc
     1381 agttcccgca ttcgccgacg cagggcggcc aactcctcgt cagtcaaacg ctgcccgccg
     1441 tcgcgctcgc aataatagcc accgaagggc atcgaatgga agatggtgat cacttgcccg
     1501 ggcagcacct cggccgcggc tacaatcatg aggaaactca acgaacgccg gtagatgcgc
     1561 gcgccatcgt tggtcgccgt cgtaaccggc accagatcgg catcttcctg caacgggaag
     1621 cacagctcgc gcaagcggcc gttcatcaag gacgcgacaa cgcgctcccc cttcggcaag
     1681 tcggccgcca atatgaactc ttcgatggga gttcctctat gcccttcaaa cacccggcca
     1741 tcgggaaaac gagcctgcac cgttgggcgt aactcggtca aatgcacttc ttcttcggga
     1801 ttcatatcga caaactcaaa gggactcgct cccatttttg aatctattca tccggcaaat
     1861 atttaccgtt tggggctcgg ataaccagct gatgcatatg tttcagccgc ataaacgctc
     1921 tatgtggggt gacaagcgat caaacctcta gaatcatcag gcaggttacg tacttattca
     1981 tcatcgatca ttccttggca acagcccacc agccgacttt tgcacggcga agcggggcgc
     2041 taatgccatc cggaaggtcg accggttccg ggagggcaat accttcacgc gggatgatca
     2101 ctgcatccaa cagtacgccg gaggtaaccg gagcatcgtc ggccgagaaa gcatagagcg
     2161 gatattgacc aaacagctcc aggtcgcgaa caaaatattg caccgtgttg ccgttcgggt
     2221 cctggccgaa gacttcggcg cggggaaagc gaaggccatt ctcctctacc catcgataga
     2281 gtcgcaagcc gccgatctcg gtaccccagt catcgagaag catggcggga agcacatgaa
     2341 gctcaggccc ctgagtaacc aatgcatagg tgagataaac cgtctccgcg tcgtcgcgac
     2401 tatcgaaacg taatgcgagg ccatctccaa acggcactaa ctctccacga acagcttctt
     2461 tgacgatcat ggtcgctctg aatcgctcat ttccaccgac ggccaaccca accggccatt
     2521 caggccactg tcggatgatc cgacgcgatt tatcaagtgt atccaagcgc cagggcattt
     2581 acaaaccgtt ccgct
//
LOCUS       DAOMQG010000443         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15962_length_2595_cov_8.237008, whole genome shotgun sequence.
ACCESSION   DAOMQG010000443 DAOMQG010000000
VERSION     DAOMQG010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15962_length_2595_cov_8.237008"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLC06_15700"
     CDS             <1..264
                     /locus_tag="PLC06_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRO91943.1"
                     /translation="LSLPGFVVVIIWQFTNIWNEFLFAVTILQKPEMQPITVALRNLS
                     GSQIKEWNVVMAGAIIAAIPTLIVYIVMGRYFVRGLLAGSVKG"
     gene            276..629
                     /locus_tag="PLC06_15705"
     CDS             276..629
                     /locus_tag="PLC06_15705"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRO91944.1"
                     /translation="MTDPTPEQPKQTRITIDLPKDLRPVYSNLAFINYTPAEIVLDFA
                     QVLPRTPRGSLLARVIMSPIHAKLLHAALAQNLAGYERQFGEIQLPQPRPNIADNFFR
                     FPGEGGGDEEDKRDG"
     gene            604..1266
                     /locus_tag="PLC06_15710"
     CDS             604..1266
                     /locus_tag="PLC06_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HRO91945.1"
                     /translation="MKKTNEMDELDTTAETIRRYMEQVDGARDLAYKRSRSLIALCAR
                     TIRAIHREEWDAAEALLAEARIATDDLVGGVRDYPELYFAGYTQDAVKEFVEGNLVYA
                     MVRGRPLPAPEDLGSEPNTWLNGLAEAASELRRRILDLLRHGETAEAERLLDAMDHIY
                     STLVTFDFADQITGGLRRRTDALRAVFERTNGDVANSYRSTQLEAALKSLELKLEKGE
                     ER"
     gene            complement(1263..2396)
                     /locus_tag="PLC06_15715"
     CDS             complement(1263..2396)
                     /locus_tag="PLC06_15715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRO91946.1"
                     /translation="MTSDGNSWADKLRAAVGQVLPWGAKQPFAPPQKALILQPCCLGR
                     VMMTTPLLAALSEAFPETRFDWAISDWALQAIGGNRRLTRTIRSGPGDLAHNSREEMR
                     DFLEAVKREGYDTCFIPSASPLAAHVARQTGIPQRVGLSAGGRAGLTLTARPPSAERW
                     TARRYLALAAAVGVLETILNTVEMEFAPTDSDRTAVVRWLVEEFDWLGDTPLVVFHPG
                     GGDNPERRDLDIRWPAYRFARLANHLQKTHGARIVLVGLAEERALAGEVAGMVPFSPA
                     NQAGQMSLGQVAALCELAGLYVGNDIGSTHVAAATGCPTLAIYGPTDPAANAPYMVNG
                     RVQTLWRPYEGEFDWANGVSVEEAVAATDTLLTAYSLGRGATV"
     gene            complement(2408..>2595)
                     /locus_tag="PLC06_15720"
     CDS             complement(2408..>2595)
                     /locus_tag="PLC06_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRO91947.1"
                     /translation="GDELRRGLESRGIRVRAGSLSGLAEEAPQAYKDVDRVIEVVHGA
                     GIARKVARLVPVAVVKG"
BASE COUNT          514 a          875 c          746 g          460 t
ORIGIN      
        1 ctgtcattgc cggggtttgt ggttgtcatc atctggcagt tcaccaacat atggaacgaa
       61 tttctgttcg ccgtgacgat cctgcaaaaa cccgaaatgc agccgatcac tgtcgcgctg
      121 cgcaatctgt ccggcagcca gatcaaggaa tggaacgtcg tgatggccgg ggcgattatc
      181 gccgccattc caacgctcat cgtctacatt gtgatggggc gttacttcgt ccgcggatta
      241 ttggccggat cggtgaaggg ataatagaat gccttatgac tgatccaaca cctgaacaac
      301 ccaaacagac tcgtattact atcgatctgc ccaaagatct gcgtcctgtc tattccaacc
      361 tggccttcat caactacacc ccggccgaaa tcgtgctcga ttttgcccag gtattgccgc
      421 gcacaccgcg cggctccctg ttggcccgtg tgatcatgag tcccatccac gccaaactgc
      481 tgcacgctgc tctggcgcag aacctggccg gctacgagcg ccaattcggc gagatacagc
      541 tgccacaacc ccgccccaac atcgccgaca acttcttccg cttccccgga gagggcggcg
      601 gtgatgaaga agacaaacga gatggatgaa ctggacacca cagccgaaac gatccggcgc
      661 tatatggagc aagtcgatgg cgcgcgcgat ctggcctaca aacgctcacg cagcctgatc
      721 gccctttgtg ctcgaacgat ccgggctatc caccgcgagg agtgggacgc agccgaggcc
      781 ttgctggccg aggcgcggat cgccaccgac gacctggtcg gcggggtgcg cgactacccc
      841 gaactatatt tcgccggcta cacccaggat gcggtcaagg agttcgtcga ggggaacctc
      901 gtctacgcga tggtgcgcgg ccgcccgctg ccggcgccgg aagacctcgg ctcggagcct
      961 aatacctggc tcaacggtct ggccgaagcg gccagtgaac tacgccgtcg catcctcgac
     1021 ctgttgcgcc acggcgaaac ggccgaagcc gagcggctgc tcgacgcgat ggaccacatc
     1081 tacagcaccc tggtgacgtt cgacttcgcc gaccagatca ccggtggtct tcgccgccga
     1141 acagacgcgc tgcgggccgt ttttgagcgg acgaatgggg atgtggccaa tagctaccgc
     1201 agcacacaac tggaagcggc gctgaaatca ctggaattga agctcgaaaa gggagaggaa
     1261 cgctaaacag tcgcccctct gccgagcgaa taagcagtca atagcgtgtc ggttgcggcg
     1321 accgcctcct ccaccgacac gccgtttgcc caatcgaact ccccctcata cggccgccag
     1381 agcgtttgga cacggccgtt gaccatgtag ggcgcattgg cggccggatc agtcggccca
     1441 tagatcgcca gcgtcgggca gccggtggcc gcggcaacat gggtagaacc gatatcgttg
     1501 cccacgtaca gccctgccaa ctcgcacaat gccgccacct gccccaggct catttgcccg
     1561 gcctgatttg ccggcgaaaa cggcaccatt cccgccactt ccccggccag ggcgcgttct
     1621 tcagccaggc ccacgaggac gatccgtgcg ccgtgcgtct tctgcaggtg attcgccagc
     1681 cgagcaaacc gataggcagg ccagcgaata tcgagatcgc gccgttcggg attgtctccg
     1741 ccgccgggat ggaacaccac caacggcgtg tcgcccaacc agtcgaactc ctccaccagc
     1801 cagcgaacca cggctgtccg gtcgctgtcc gtgggcgcaa actccatctc gactgtatta
     1861 aggatcgtct cgaggacacc aactgctgct gccagggcca gatagcggcg agccgtccag
     1921 cgctcggccg acggcgggcg ggcggtcagg gtaagccctg ctcgcccgcc cgcgctaagc
     1981 ccgacgcgct gcgggatgcc tgtttgccgg gcgacgtgcg ctgccaaagg tgacgcagac
     2041 gggataaagc acgtgtcata cccctccctt ttcacggcct ccaggaaatc gcgcatctcc
     2101 tcgcggctat tatgagccaa atcgcccggc ccgctgcgta tggtgcgcgt gaggcgccgg
     2161 ttgccaccaa tcgcctgtaa ggcccaatca ctgatagccc agtcaaaacg cgtctcaggg
     2221 aatgcctcgc tcaatgcggc cagcagtggc gtggtcatca tcacccgtcc caggcaacag
     2281 ggttgcagga taagcgcctt ttgcggtggg gcaaaaggtt gtttggcgcc ccacgggaga
     2341 acctgtccca ccgcggcgcg caacttgtcc gcccaggaat tgccgtctga agtcacgatg
     2401 tccgcctcta tcccttcacc actgctacgg gcacgagccg cgccaccttg cgggcaatcc
     2461 ccgcgccgtg gaccacttca atcacccgat cgacatcttt atacgcctgt ggcgcttctt
     2521 ccgccagccc cgacaggctg ccggcgcgca cgcggatgcc gcgactctcc agcccgcgcc
     2581 gcaactcgtc gccct
//
LOCUS       DAOMQG010000444         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_15978_length_2593_cov_12.869582, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000444 DAOMQG010000000
VERSION     DAOMQG010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15978_length_2593_cov_12.869582"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..942)
                     /locus_tag="PLC06_15725"
     CDS             complement(145..942)
                     /locus_tag="PLC06_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO91948.1"
                     /translation="MTKFATLEQLISLRGKRALITGAAGGIGKAIACRFAEAGADLEL
                     VDRDEATLAGAANELSGYGVRIRSHVVDLSNKEQIDALWNSLEGNEPDILVNNAGIYP
                     FRQFVDSDEEFVRHLMEVNLFSVTWMCREMIARRRKLGGVIINLGSIEAVLPFKEDAA
                     HYSVSKAGVIALTRALAKEHARHGFRVNAILPGGILTPGTKAAAKEIFRFKFDLIRTG
                     IEFRQRLPIGRVGRPDEVARMALVLASDLASYVQGAAIPVDGGFLAA"
     gene            1002..1355
                     /locus_tag="PLC06_15730"
     CDS             1002..1355
                     /locus_tag="PLC06_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91949.1"
                     /translation="MGELLRNAENAYIDPRKLRDYALSLSHDSGRYKAAFFAQMGYNA
                     EKWWQLENDIREQHLTQRAEEGSASPFGIKYIITAQIQGPSGESRWVTTVWIFRPGED
                     RAHLVTIEAAKERSG"
     gene            1352..1600
                     /locus_tag="PLC06_15735"
     CDS             1352..1600
                     /locus_tag="PLC06_15735"
                     /inference="COORDINATES: protein motif:HMM:NF027602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HRO91950.1"
                     /translation="MNSQQLELLDTVIVTVDLPSEEVLAGDLGTIVEIYNAPSPAYEV
                     EFVNPDGTTRALLTLLPEEIRPLSAMDVLTTRQVMLAG"
     gene            complement(1726..2214)
                     /locus_tag="PLC06_15740"
     CDS             complement(1726..2214)
                     /locus_tag="PLC06_15740"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HRO91951.1"
                     /translation="MIVVSNTTPLIGLASIEQFGLLQDLFGEIIISRAVYDESVIAGR
                     DEGGARREVAASAWITTVEIKDRLAVDVLLDELDLGEAETIVLARELGADWVLMDERK
                     GRRKLSQLGIPKIGTIGILLLAKNRGLISVVAPQIERLRQHGFTISQAVVDAILEEAK
                     ES"
     gene            complement(2211..2492)
                     /locus_tag="PLC06_15745"
     CDS             complement(2211..2492)
                     /locus_tag="PLC06_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HRO91952.1"
                     /translation="MQENTIQIRVPHSLLEYGLRAEEIQEQVAEWLTLSLFVDGRISS
                     GKAASLLGLSRIQFLQLLRRRGISYIDYAPEEVAEESAAANTLTPIGPE"
BASE COUNT          640 a          736 c          673 g          544 t
ORIGIN      
        1 tcggttcgca tgcccagcgc ccacatggcc ggcaggaaat cctgggcctg gacggcgccg
       61 aagaaagcga cgacatcaac gggggtcggg aacttcggcc caatgaggcg ttgggtatgg
      121 agacgacgtt gtgcgatcat gaaatcaggc cgccaaaaaa ccaccgtcaa ccgggatagc
      181 cgcgccctgg acgtagctcg ccaaatcgct ggccagcacc agagccatcc gcgccacttc
      241 atccggtcgg ccgacgcggc cgattggcag gcgctgcctg aactcaatgc ccgttcggat
      301 gagatcgaac ttgaagcgga atatctcttt ggcggcggct ttcgtccccg gcgtcaggat
      361 accacccggc aggatagcgt tgacccggaa gccatgccgg gcatgttcct tggccaaggc
      421 tctggtcagt gcgataacac cggccttgct cacgctgtag tgggctgcat cctctttaaa
      481 gggcaacacc gcttcgatcg aacccaggtt gatgatcacg ccgccgagct tgcgccgccg
      541 ggcgatcatc tcccgacaca tccacgtcac cgagaagagg ttgacttcca tcaaatggcg
      601 gacgaactcc tcgtcggaat cgacaaactg acgaaaagga tagatgccgg cattattgac
      661 cagaatatcc ggctcgttgc cctcaagcga gttccacagg gcgtcaattt gctccttatt
      721 ggacagatcg acgacgtgac tgcggatacg cacgccataa ccggacaact cattggcggc
      781 gccggccaat gtcgcctcgt cgcgatcaac aagttccagg tcggctcccg cctcagcgaa
      841 gcggcaagcg atggccttgc cgatgccgcc ggccgccccg gtgatgaggg cgcgcttgcc
      901 gcgaagggag attagttgtt ccagggtcgc aaactttgtc atatactccc ccaagccatc
      961 gataaaccct aaaacattga tcgttgtggt aaaatgggtt gatgggtgaa ttgctaagga
     1021 atgctgaaaa cgcttacatc gatcctcgca aacttcgcga ctacgcccta agtcttagcc
     1081 acgattcggg tcgatataaa gcggcattct tcgctcaaat gggctacaat gccgaaaaat
     1141 ggtggcaact cgagaatgat attcgcgagc aacatttgac tcaaagagcg gaagaagggt
     1201 ccgcctcacc tttcggcatt aagtatatca tcactgccca aatacagggc ccgtccggag
     1261 aaagtcgttg ggtaacgaca gtttggatat tccgccccgg cgaagatcga gcgcatctcg
     1321 tgactatcga ggcggcgaag gagcgttcag gatgaatagc caacaactcg aattgctgga
     1381 taccgtgatc gtaactgtcg atttaccttc cgaagaggta ctggccggtg atttaggcac
     1441 aatcgtcgag atctataacg ctccatcccc ggcttatgag gtcgagtttg tgaatcctga
     1501 tgggacgaca agagcactat tgaccctatt gcccgaagaa atacgcccgc tgtcggcgat
     1561 ggatgttttg acaacacgcc aggttatgtt ggctggataa gtcgacggta gctccacaac
     1621 gcggtcatcc caccaccaac gcaagcaaac ttaactgcat aaaagcagta ttgcagcatg
     1681 taccaatagg catacatcag tgcttccaat acctgtgtgc agtttttagc tttctttcgc
     1741 ctcctccaaa atcgcatcca caactgcttg gctaattgtg aatccgtgtt gccgtaaccg
     1801 ctcaatctgg ggagcaacta cggaaatcaa cccacgattt ttcgctaaca ggaggatgcc
     1861 aatcgtaccg atcttgggga tacctaattg actcagcttg cggcgtccct tgcgctcatc
     1921 cattaatacc caatctgccc caagttcgcg ggcaagcacg atggtttccg cctcgcccaa
     1981 atcgagttca tcaaggagaa cgtcaacggc aaggcgatcc ttgatctcaa cagttgtgat
     2041 ccatgcggag gccgcaactt cacgcctggc gccgccttca tcacggccgg caataacgga
     2101 ctcatcgtag actgctcgcg atatgatgat ctcgccgaac aaatcctgca acaagccaaa
     2161 ctgttcaatt gaagccagcc cgatcagggg cgtcgtgttg gaaacgacga tcattccggg
     2221 ccaatggggg taagcgtgtt ggcggccgcc gattcctcag ccacctcctc gggagcataa
     2281 tcaatatagc tgatcccacg ccgtcgaagc agttggagaa attggatacg actaaggccg
     2341 aggagagatg ccgccttgcc ggatgagatg cggccgtcga caaaaagaga caaggtgagc
     2401 cattcagcta cctgctcctg aatttcttct gcacgcagac catattcaag caacgaatgt
     2461 ggaacacgaa tctggatagt gttttcttgc ataattatct acctgctacc caaattgtcg
     2521 gtcattaatg aacagtatag cataatcggt tgggtagacc tatcccccga ccaccaaccc
     2581 caccacctca aac
//
LOCUS       DAOMQG010000445         2579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16077_length_2579_cov_10.161648, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000445 DAOMQG010000000
VERSION     DAOMQG010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16077_length_2579_cov_10.161648"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLC06_15750"
     CDS             <1..279
                     /locus_tag="PLC06_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRO91953.1"
                     /translation="LGAHGLISGLSTAIPEPFVAMTRAFAAGDIDEARAWQRCINRLL
                     GVLAGVRVGGIKAILNQRGIEVGPPVPVLDAVDEPLWARAEEQLGIRN"
     gene            329..607
                     /locus_tag="PLC06_15755"
     CDS             329..607
                     /locus_tag="PLC06_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91954.1"
                     /translation="MTRKKLVIDDIAFEPGEPTIVPLDRLFAWVVWQFPRPREDGFNG
                     AVHPPEADHGWYPAIINSAEGNAKIFGHVKEPYPTPEAAAKQLEKVRN"
     gene            complement(626..1885)
                     /locus_tag="PLC06_15760"
     CDS             complement(626..1885)
                     /locus_tag="PLC06_15760"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRO91955.1"
                     /translation="MPFMKNVLGLILGGGQGTRLYPLTKERSKPAVPLAGKYRLIDIP
                     MSNCFHADIEKIAILTQFNSASLHRHIWNTYTRDQFSPGWIQILAAEQTPRSRDWYQG
                     TADAVRKQWIEIKEAGTKYVLILAGDHLYRMDYRKFIQRHIDLDAEITVAVQPVDRES
                     VSGLGILKMGTDSQITRFAEKPKDPEVLKQFESAAHPEKPFLASMGIYVFNMSTLEDV
                     LEADFVDFGRDILPQSLEKRRVCGYTFEGYWEDIGTIRRFFEVNLRMAKLDAPFDFYD
                     PQAPIYTHARFLPGSEVHNSRLDEVLLSDGCRIYDATVRESVVGLRSIIGREANIQST
                     VLMGADHYETEEELAENRRLGRPDFGIGENSVIKGAIIDKNARIGKNVTIRYIPDRPE
                     EETDNWVARDGIVIVPKGAIIPDNAVV"
BASE COUNT          513 a          705 c          831 g          530 t
ORIGIN      
        1 ctgggcgcgc atgggctgat cagcggcctg agcacggcca tcccggagcc gttcgtcgcc
       61 atgacccgcg cctttgcggc gggggacatc gacgaggccc gcgcgtggca gcggtgcatc
      121 aatcgactgc tgggcgtgct ggccggcgtg cgcgtgggcg gcatcaaggc catcctgaat
      181 cagcgcggca tcgaggtcgg cccccccgtg ccggtgctgg acgcggtcga tgagccgctg
      241 tgggcaagag cggaagagca attgggaatc aggaattgaa gagcgcgccc ctttcgtaat
      301 tcgtaattcg ccacaggagc atgatgccat gacgcgcaaa aaacttgtca tcgatgatat
      361 cgcgtttgag ccgggcgagc cgaccatcgt gccgctggac aggctgtttg cctgggtcgt
      421 gtggcagttc ccgcgccccc gcgaggacgg cttcaatggg gcggtgcatc cgccggaagc
      481 cgaccacggc tggtatccgg cgatcatcaa ttcggccgaa gggaacgcga agatcttcgg
      541 gcatgtcaag gaaccgtatc cgacgcccga agccgcggcg aaacagctgg agaaagttag
      601 gaattaggaa ttcgtaattg gttccctaaa caaccgcatt atccggaata atggccccct
      661 tgggcacgat gacgatgccg tcgcgcgcca cccaattgtc cgtctcctct tcggggcggt
      721 cggggatata acgaatggtg acgttcttgc caatgcgggc gttcttgtcg atgatcgccc
      781 ccttgatgac cgaattctcg ccgatgccga aatcggggcg gccgaggcgg cggttttcgg
      841 ccagctcttc ttccgtctca taatggtcgg cgcccatcag caccgtcgac tggatgttgg
      901 cttcccggcc gatgatgctg cgcaagccca ccaccgattc gcgaacggtg gcgtcataga
      961 tgcggcagcc gtcagacagc aaaacttcgt ccagccggga gttatgaacc tcggagccgg
     1021 gcaggaagcg ggcgtgggta tagatggggg cctgcgggtc gtagaagtcg aagggggcgt
     1081 cgagcttggc catgcgcagg ttgacctcga agaagcggcg aatcgtcccg atgtcttccc
     1141 agtagccctc gaaggtgtag ccacacacgc ggcgcttttc gagggattgc ggcagaatgt
     1201 cgcggccgaa atccacaaag tccgcttcca ggacatcttc cagcgtgctc atgttgaaga
     1261 cgtagatgcc cattgaggcc aggaaaggct tctcggggtg ggcggcgctc tcaaattgct
     1321 tcaggacttc ggggtctttc ggcttctcgg cgaagcgggt gatctggctg tcggtgccca
     1381 tcttgagaat acccaggccc gacaccgatt cgcggtcgac gggctggacg gccacggtga
     1441 tttcggcgtc gaggtcgatg tggcgctgga tgaacttgcg gtagtccatg cggtagagat
     1501 ggtcgccggc gaggatcagg acgtatttcg tgcccgcttc cttgatctca atccattgct
     1561 tgcgaacggc gtcggccgtg ccctgatacc agtcacggct gcgcggcgtc tgctcggcgg
     1621 ccagaatctg aatccagccc ggcgagaact ggtcgcgggt gtaggtgttc cagatgtggc
     1681 gatggagcga ggcggaattg aattgggtca ggatggcgat cttctcgata tcggcgtgga
     1741 agcagttgct catggggata tcgatcagcc ggtatttgcc ggccaagggc acggcgggct
     1801 tcgagcgctc cttggtcagc gggtagaggc gtgtgccctg cccaccaccc agtatcaggc
     1861 cgagaacatt tttcatgaag ggcataatct ctccgttagc gcgtcagttg aaaaggagtt
     1921 ggcgaactat agcgctacct gatgaaacgc gcacattttt gtagcgcaga cattcttgtc
     1981 tgcggggggc gcagggaaaa gaggcaatca ggacagattg cagggcaatt gtatgaaaaa
     2041 tattggaccg cgaatgaacg cgatttaacg cgaatgaacg cgatttaacg cgaatgaacg
     2101 cgatttaaat ggacagaaat aacctttgag atccgacaaa acaggctgat gtcgggaatc
     2161 ctgcctcgca gccacccgcc atatccgtgt tcattcgcgt tcattcgcgt tcattcgcgg
     2221 tccaatattc ttcatgcgtt gccttgggac agattgcgct acggtgtggc cccacatcgg
     2281 tgtggcccca catcagtgtg gtccacatca gtgtggccca catcgcccgg acatgttatg
     2341 attttgtcgc gccccaatcg gcattcgtgt cgaggggcga cgattttgtt atcaggagag
     2401 ttcatgagtc gagtgattca tgtggacaac cccggcaagc gccgcaatca ggcgcgccgt
     2461 tctatcgcgg agatactgcg cctgctgggg cgcaagcagg ccatcgacga tgagggccgc
     2521 gatatggccg ccatgctggt ctactttctg cgcgagatca aggagacggt ggacgaatc
//
LOCUS       DAOMQG010000446         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16194_length_2564_cov_9.541650, whole genome shotgun sequence.
ACCESSION   DAOMQG010000446 DAOMQG010000000
VERSION     DAOMQG010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16194_length_2564_cov_9.541650"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="rfbG"
                     /locus_tag="PLC06_15765"
     CDS             complement(<1..1019)
                     /gene="rfbG"
                     /locus_tag="PLC06_15765"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRO91956.1"
                     /translation="MTFNNIYSGKTVLITGHTGFKGSWLTTWLLELGASVVGYSHPEP
                     PTQPSNFVTSGLAGHITDVRGDIRNYDHLLKTITDHRPEIIFHLAAQPIVLRSVELPK
                     LTIDTNTGGTVNVLEAIRHADSVRALVSITTDKVYENQEWLWGYRETDQLGGHDPYSA
                     SKAMAELAIAAYRSTYFPPEQYGHAHRVAIASTRAGNVIGGGDFADYRLVPDCMKALM
                     DGRPIGVRNPLSVRPWQHVLEPLSGYLWLGAQLLENGPACGEAWNFGPLEQKGIPAQA
                     LAEKLVELWGSGSWIHTHPGYAEVETGLLRLSWDKAAHRLNWRPVYAWVDALQEITDW
                     FKAFQA"
     gene            complement(1141..2295)
                     /locus_tag="PLC06_15770"
     CDS             complement(1141..2295)
                     /locus_tag="PLC06_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO91957.1"
                     /translation="MHIAINAYFWNRPNTGSGQYTRQLVYHLNRLVEDMKITLVFPVT
                     PGDAEPADVPPDVHVERVAMRPGNPGKVLFEQRGFPAACRRVGADLAHVPYWGPPLRS
                     PVPLVVTIHDLTTLLVPEYNRGARVRLYNALVSAAARGADHVLTDSFSSKLDIMDHLG
                     IDERDITAIYLGVGSQYAPHPAAGDNSLIDMAVVQKYDLPESYVLYLGGYEIHKNVPT
                     LLQAYTYVARGLGESYPLVLAGKKPDAVSDVYPDYDGIIERAGLSKYVRWIGYVDEED
                     KPLLYRNAEVFVFPSRREGFGLPLLEAMACGTPVVASNGGSLPEIIGPAGFAFDPDDA
                     RNMAGAIISLVVQESLAPDLRRQGLEQAARFTWDNTAIETLKVYDHVLRK"
BASE COUNT          455 a          750 c          860 g          499 t
ORIGIN      
        1 gcctgaaaag ccttgaacca gtcggtgatc tcttgcagcg cgtcgaccca ggcatagacc
       61 ggccgccagt tcagccggtg ggcggccttg tcccagctga ggcgcagcag tcccgtctct
      121 acctcggcat agccggggtg ggtatggatc cacgagccgc tgccccaaag ctcgaccagc
      181 ttctcggcca gcgcctgtgc ggggatgcct ttttgctcca gcgggccgaa gttccaggcc
      241 tcgccgcagg ccgggccgtt ctccagcagt tgtgcgccca gccataagta gccgctgagc
      301 ggctccagta catgctgcca cggccgtacg ctgagcgggt tgcgcacgcc gatggggcgg
      361 ccgtccatga gcgccttcat gcagtcgggc accagccggt agtcggcgaa gtcgccgccg
      421 ccgatgacgt ttccggcgcg cgtcgaggcg atggcaacgc gatgggcatg gccgtactgc
      481 tctggcggga agtaggtgct gcgataggcg gcgatggcca gctcggccat ggccttgctg
      541 gcgctgtagg ggtcgtggcc gcccagctgg tcggtctccc gatagcccca cagccactcc
      601 tgattctcat acaccttgtc ggtggtgatg ctgaccaggg cgcggacgct gtcggcatgg
      661 cggatggctt ccagcacgtt gaccgtgccg ccggtgttgg tgtcgatggt cagcttgggc
      721 aactcgaccg agcgcagaac gatgggttgc gcggccagat ggaagatgat ctccggccgg
      781 tggtcggtga tcgtcttgag caggtggtcg tagttgcgga tgtcgccgcg cacgtcggtg
      841 atatgcccgg ccagcccgga cgtgacgaag ttgcttggct gcgtcggcgg ctccgggtgg
      901 ctatagccga cgacactggc gcctagctca agcagccagg ttgtgagcca ggagcctttg
      961 aagccggtgt ggccggtgat gagtacggtt ttgcctgaat agatgttgtt gaaggtcatg
     1021 tttgatttcc agtcgtcttc agtcagtggt cagtgggtca gtaggtcagt ggtcagttgg
     1081 ttagtaggtc agttggtcag tgggtcagtg ggtcggtggt cagagatcac tcgtcattcg
     1141 tcacttcctc aacacatgat catacacctt cagcgtctcg atggcggtgt tgtcccaggt
     1201 gaagcgggcc gcctgttcga ggccctgacg acgaagatcg ggggcaaggg actcctgcac
     1261 gacaaggctg atgatggccc cggccatatt gcgcgcgtcg tcggggtcga aagcgaaacc
     1321 ggccgggccg ataatctccg gcagcgagcc gccgttgctg gccacgacgg gcgtgccgca
     1381 agccatcgct tccagcaagg gcagcccgaa tccctcgcga cggctgggaa agacaaagac
     1441 ctcggcgttg cgatagagca gcggcttgtc ttcctcatcc acgtagccga tccagcgaac
     1501 atatttcgat aacccggctc gctcgatgat cccgtcataa tcgggataga catcggaaac
     1561 ggcgtcgggc ttctttccgg ccaataccaa tggataactc tcgcccagcc ccctggccac
     1621 ataggtgtag gcctggagca aggtcggcac gttcttatgg atctcgtagc cgcccaggta
     1681 aagcacgtag gattcgggca ggtcatactt ttgaaccaca gccatatcga tcaggctgtt
     1741 gtcgccggcg gccgggtggg gggcatattg cgagccgaca ccgagataaa tggcggtgat
     1801 atcccgctcg tcgatgccca agtggtccat gatatcgagc ttgctggaga aggagtcggt
     1861 caacacgtgg tcggccccgc gcgcggcggc gctcaccagg gcgttgtaga gccgaacgcg
     1921 cgcgccgcga ttgtattccg gcaccagcag cgtcgtcagg tcgtggatcg tcaccaccag
     1981 cggcaccggc gaacgcagcg gcggccccca gtatgggacg tgggccagat ccgcccccac
     2041 gcggcggcag gcagcaggga agcctcgctg ctcgaacagg actttgcccg gattccccgg
     2101 ccgcatggcg acgcgctcta catgaacgtc gggcggcaca tcggccggct ccgcgtcgcc
     2161 gggcgtgacc ggaaagacca gggtgatctt catatcttcg accagccggt tgagatgata
     2221 gacgagttgg cgcgtgtatt ggccgctgcc ggtattcggc cgattccaga aataggcgtt
     2281 gatggcgata tgcattgggc ctgattataa gccaggcgcg aacagacggg tagcgcgggc
     2341 atccgactcg tggcacaagc tgccggtttg tgaatgggcg aagaggcaag ttggcaactt
     2401 gcgccacatt gccgtcggta gtgctgcctc aactttaccg ggtgagtgcg gtatggtata
     2461 gttgccgcgc ggatcggatc ggcgttaacc ggatggcaga acgagaaaaa cagttcactc
     2521 ccggcctggg aactctcctg ctcgcgatca ttcttctggg cgcg
//
LOCUS       DAOMQG010000447         2563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16200_length_2563_cov_10.448166, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000447 DAOMQG010000000
VERSION     DAOMQG010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16200_length_2563_cov_10.448166"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLC06_15775"
     CDS             complement(<1..444)
                     /locus_tag="PLC06_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRO91958.1"
                     /translation="MIDKFLVDTNVLVYVYDRSEAEKQNLAEVVCDRLVLSGRGILST
                     QIMSEFFNATTRRLAEPLPLDLAYRRLENYHRSWEVVNITPTIVLEAARGAIDHQLSF
                     WDALIWATAKLNQVPVILSEDFRSGTSLEGVRFVNPFGRDFDLQTW"
     gene            complement(434..691)
                     /locus_tag="PLC06_15780"
     CDS             complement(434..691)
                     /locus_tag="PLC06_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRO91959.1"
                     /translation="MSTKIRKQIYLDRDQELQLKRLAAAIGSSEAEIIRHSLDSRLGM
                     PKQLPRDLSAWEEEKQFIEDWIARGTVGGGRIWKREDLYDR"
     gene            complement(816..1253)
                     /locus_tag="PLC06_15785"
     CDS             complement(816..1253)
                     /locus_tag="PLC06_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRO91960.1"
                     /translation="MTEQTQVAERPFTVAAFQAYMDEGKLMGSRNRETGEMFVPPRPI
                     DPHTHSDDMEWVEMSGRGTLVAFTSVYIGTSAMIDAGYDRSKPYVAGIVELEEGPRIS
                     AQIVGVDGKQPDIAWIGRPVRATFIERGEGEKRRAFLGFEFTS"
     gene            complement(1319..>2563)
                     /locus_tag="PLC06_15790"
     CDS             complement(1319..>2563)
                     /locus_tag="PLC06_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91961.1"
                     /translation="RGVAIVGTGMSKFGSFPDKTTRDLFVEAYQDMGTAVDKGFDPNE
                     IEAVYIGNYSSDLFEGQGHTAPIMADWVGLAPRAATRIEDACASSGVALRQGILAIAS
                     GAYDMILVGGIEKMSNLPIAGVTDALATAGDVSFEIPAGFTFPGFYGAMASAYIHRYG
                     ASADALLRVGIKNHDNGKLNPKAQFQARIADLMAARIAKAQQKGLPVPVWQTEMDFLH
                     DDGANPYIAWPLRLFDCSPVTDGASAVLLVGEELAKRFTDDPLYIIGSGQASDAAALH
                     DRAELTGIRAAQIASRQAYEMAGVTPQDIKIAEVHDCFTIAEIIATEDLGFFAPGTGH
                     EAAEEGLTTRHGPKPINTSGGLKSKGHPVGASGAAQAVEVWKQMRGQAGERQVDRDVD
                     LAMTHNVGGTGQTAVVHIYERR"
BASE COUNT          482 a          783 c          756 g          542 t
ORIGIN      
        1 ccatgtttgg agatcgaaat cgcggccaaa aggattgaca aaacggactc cctcgagcga
       61 tgtgcccgat ctgaagtcct cgctcaggat aacgggaact tggttcagtt tggctgtggc
      121 ccagattagc gcgtcccaga agctcaattg atggtcgatc gcccctctgg ccgcctcaag
      181 cacgatcgtt ggcgtgatat tgactacttc ccagctacga tgatagttct caagccgtcg
      241 ataggctaaa tccaatggca ggggctcggc caggcggcgc gttgtcgcgt tgaagaactc
      301 agacatgatt tgcgtgctca aaataccgcg gccactcaac accagccggt cgcagaccac
      361 ctcggccaaa ttctgctttt ccgcctctga tcgatcgtag acatagacga ggacattggt
      421 gtcgacaagg aacttatcga tcataaagat cctcgcgctt ccagatacga ccgccgccga
      481 ccgttcccct ggcgatccaa tcctcaatga attgcttttc ctcttcccat gcagacagat
      541 cgcgggggag ttgctttggc attccgaggc gagagtcgag actgtggcga atgatttcag
      601 cctccgacga cccgatagcc gccgccaatc tttttagttg tagctcctga tcgcgatcca
      661 gataaatttg tttgcgaatc ttggtcgaca tggtctcttc atcacctccg tatacgtcat
      721 attatacatt atcccctgat ggaaaaacaa aatcgcgcgg gattgttgcg aatcccgcgc
      781 gattattatt tattatgaag atttagcgag ccgtgttacg aagtaaactc aaatcccaga
      841 aatgcccgtc gcttttcacc ctcgccccgc tcgatgaatg tcgcgcggac cggacggccg
      901 atccaggcaa tatcgggctg tttgccgtcc acgccgacga tctgggcgct gatgcgcggc
      961 ccttcttcca gttcgacgat cccggctacg tagggtttgc tgcggtcgta gccggcgtcg
     1021 atcatcgccg acgtcccaat atagaccgat gtaaaggcga ctaaagtgcc tcggcccgac
     1081 atctcaaccc attccatatc atcgctatgg gtgtgggggt cgatcggccg tggcggcaca
     1141 aacatctcgc ccgtctcgcg gttgcgcgaa cccatcagct tgccttcgtc catgtaagcc
     1201 tgaaacgccg ccacggtgaa tgggcgctct gctacttgtg tttgctcagt catgattaac
     1261 ctgtttaccg atcactgaca actgccctac tgaatactgc cccactgaac accggctgct
     1321 accgccgctc ataaatatgc acaacggccg tctgccccgt gccgccgacg ttgtgggtca
     1381 tggccagatc gacatcacgg tcaacctgac gctcgccggc ctggccgcgc atctgcttcc
     1441 aaacctcgac cgcctgggcc gcgccggacg caccgaccgg gtgacctttc gacttgaggc
     1501 cgccggaggt gttgatcggc tttggcccgt ggcgcgtggt cagcccttcc tcggccgctt
     1561 cgtggcctgt gcccggcgcg aagaagccca ggtcttcggt ggcgatgatc tcggcgatgg
     1621 tgaagcagtc gtggacttcg gcgatcttga tgtcctgagg cgtcacgccg gccatctcgt
     1681 aggcctggcg ggaggcaatt tgcgcggcgc ggatgccggt tagctcggcc cggtcgtgga
     1741 gtgcggccgc gtcgctggcc tgcccgctgc cgatgatata gagcgggtcg tcggtgaagc
     1801 gtttggccag ctcctcgccc acgagcagaa cggccgaagc cccatcagtc accggggagc
     1861 agtcgaacaa ccgcagcggc caggcgatgt aggggttagc cccgtcgtcg tgcaggaagt
     1921 ccatctcggt ctgccagacc ggcacgggca ggcctttctg ttgagccttg gcgatgcgtg
     1981 cggccatcag gtcggcgatg cgcgcctgga actgggcctt agggttcagc ttgccgttgt
     2041 cgtggttctt gatgcccaca cgcagcaggg catcggccga cgcgccatag cggtggatat
     2101 aggccgaggc catggctccg tagaagccgg ggaaggtgaa cccggccggg atctcgaagg
     2161 acacgtctcc ggccgtggcc agtgcatcgg tgacaccggc gatgggcaga ttgctcatct
     2221 tctcgatgcc gccgacgaga atcatgtcat acgcgccgga ggcgatggcc aggataccct
     2281 ggcgcagggc aacgccgctg ctggcgcagg cgtcctcgat gcgcgtcgcc gcccggggcg
     2341 caagccccac ccagtcggcc atgatcgggg cggtgtggcc ctggccctcg aacaggtcgc
     2401 tgctgtaatt gccgatgtag acggcttcaa tttcattggg gtcgaagccc ttgtccacgg
     2461 cagtacccat gtcctggtag gcttcaacaa acaggtcgcg cgtcgtcttg tcggggaatg
     2521 agccgaactt gctcatgccc gtgccgacga tggcgactcc acg
//
LOCUS       DAOMQG010000448         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16376_length_2544_cov_7.257131, whole genome shotgun sequence.
ACCESSION   DAOMQG010000448 DAOMQG010000000
VERSION     DAOMQG010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16376_length_2544_cov_7.257131"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLC06_15795"
     CDS             <1..320
                     /locus_tag="PLC06_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO91962.1"
                     /translation="QYGFWKVRVMQKHPRQMRLRQFVPPAFVAALLGSLLFGMFLRPF
                     RYVLAFILILYVMADLSASLSLGRAHGPRHVPRLLMIHPILHLSYGLGFLAGLRHFAG
                     RRE"
     gene            350..2281
                     /locus_tag="PLC06_15800"
     CDS             350..2281
                     /locus_tag="PLC06_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91963.1"
                     /translation="MSHTNQSNTGKTGITRRRFLQQSAVVMSGLALQRTWMGAAALRL
                     GRALQGADAINVMDPAYGAKGDGKTNDRDAFQAAIDAAVEAGVPLYIPRPPQFYRIGL
                     DPNHDRLLVAGDLAIVGDGRVTTTLRFTVNTTASGKNYSAFFVPGGVNFGIANLRLEE
                     DLHQPVEQFEFMGVFLQPSKADNTCVVESVDIDGFTHCLYSPSSGDDSSDGELFLTVR
                     DCDLHPWWTFCIGLWTVPGGHKRLHIYDSYLHDNQNGHLVYTHPHNSVYVENTRFDGA
                     SSWAFHFQGTEVAGDPEYQRFVGCWFGPRNTQGLITQDRATVATEVEVSNCMFEGQPS
                     IQIRSDIVIDGCYFTTSQDSARDALFIGAYSNAPWRAIIRNCIFAPKSNMLPQVDMRL
                     ENIDVTIENCQFFNQRSGTMLTLGGGTMNRYTVQDCVFYNRPDNASQSIAIEVDNGQT
                     VIDNCRFFGRAIGDRGVIILHSTDIGPAADSFLQIDNCTFKDVSGGTVFYALMPTANS
                     WSGKFAGTNNHIVNLQTGKPLLIVDPPAPVYGRLTPVAAVAPTSIPAGPMMILSSNYD
                     TYEVLGSADVATLHWWTADGLSDPLFSGTVTLKATVPFALVTGGNIQLAGGAARRDVA
                     AGASVRLTYDSAAGAWSEG"
     gene            2286..>2544
                     /locus_tag="PLC06_15805"
     CDS             2286..>2544
                     /locus_tag="PLC06_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRO91964.1"
                     /translation="MGGPAGSDETARVREVYAERAERLAGAGTYSLTNDAYLFAIQSR
                     QRALLRMLRREGMWPLAGKDILEVGCGNGGVLLELLGYGADP"
BASE COUNT          468 a          751 c          798 g          527 t
ORIGIN      
        1 atcagtacgg cttctggaag gtgcgggtca tgcagaagca cccgcggcag atgcgactgc
       61 ggcaatttgt gccgccggcg ttcgtcgccg ccctgctggg ctccttactg tttggtatgt
      121 ttctccggcc gttccgctac gtgttggcct ttattctgat attgtatgtc atggccgacc
      181 tgagcgcgtc tctttcactc gggcgagcac atggtccacg ccacgtacca cgattgctga
      241 tgatccaccc gatccttcat ctaagttatg ggttgggttt cctggcgggc ctgcgacatt
      301 tcgccggacg acgggaatga tgcgccacgg ccgcccaaag cgagtaacaa tgagtcacac
      361 caatcaatcc aacacaggca agacaggtat cacgcgccgg cggtttttgc agcagtcggc
      421 cgtcgtcatg agcggtctcg cgcttcagcg aacctggatg ggtgcggccg cgctgcgcct
      481 ggggcgggcg cttcagggcg cggatgccat caacgtcatg gacccggctt atggcgcgaa
      541 aggtgacggc aagactaacg accgcgacgc ctttcaggca gctatcgacg ccgccgtgga
      601 agccggagtg ccgctctata ttccacggcc gccacagttc taccgcatcg ggctggatcc
      661 gaatcacgat cggctgctgg ttgccggcga cctggctatt gtgggcgacg ggcgcgtgac
      721 gaccaccctg cgcttcacag tgaatacaac ggcgagtggc aagaactatt ccgccttctt
      781 tgtgccgggc ggggtcaatt tcgggatagc caatttacgg ttggaggagg atctccacca
      841 accggttgag cagttcgaat tcatgggcgt gtttttgcag ccgtccaaag ccgataacac
      901 ctgcgttgtg gaatcggtgg atatcgatgg gttcacccac tgcctctact ccccctcgtc
      961 gggcgacgat agcagtgatg gcgagctgtt cctgaccgtg cgcgattgtg atttgcatcc
     1021 gtggtggaca ttttgcatcg gtctgtggac tgtgccgggc gggcacaagc gcctgcatat
     1081 ttacgacagc tatctccacg acaaccagaa cggccatctg gtgtataccc acccccataa
     1141 ctcggtctac gtcgagaaca cgcgtttcga cggggccagt tcgtgggcat ttcacttcca
     1201 gggcacggag gtggccggcg atccggagta tcagcgcttc gtcggctgct ggtttggccc
     1261 gcgcaacacc caggggctaa tcacccagga tcgggcgacg gtcgccactg aagtggaagt
     1321 gagcaattgc atgtttgagg gccagccttc gatccagatt cgctccgata tcgtgatcga
     1381 cggctgttat ttcacgacga gccaggactc ggcccgcgac gcgttattca tcggggccta
     1441 ttccaacgcg ccgtggcggg cgataattcg taattgcatc ttcgcgccga agtccaacat
     1501 gctgcctcag gtcgatatgc gcctggagaa tatcgacgtg accatcgaga attgccagtt
     1561 cttcaaccag cgctccggga cgatgctgac cctggggggc gggacgatga accgctacac
     1621 cgttcaagat tgcgtgttct acaaccggcc ggacaatgcg tcgcaatcga tcgccattga
     1681 agtcgataat gggcagacgg tgatcgacaa ctgccggttc ttcggccgcg ccatcggcga
     1741 tcgtggggtc attatccttc acagcaccga cattggcccg gcggccgact catttctgca
     1801 gatcgacaac tgcacgttca aggatgtgag cggcggcacg gtgttctacg cgcttatgcc
     1861 cacggccaat agctggagcg gaaaattcgc gggcaccaat aatcacatcg tcaacctgca
     1921 gacgggcaag ccgctgctca tcgtcgatcc gccggcaccg gtttacggtc gcctgactcc
     1981 ggtggcggcc gtcgctccaa cctcgatacc cgccgggccg atgatgatcc tgtccagcaa
     2041 ttacgatacc tatgaggtgc tggggtcggc cgatgtcgcc accttgcact ggtggacggc
     2101 cgatgggctg tccgacccat tattcagcgg gacagttacc ctgaaagcga ccgtaccctt
     2161 tgctctcgta accgggggaa acatccagtt ggcgggcggc gcggcgcgcc gcgatgtcgc
     2221 tgccggcgct tcggtgcgac tcacttatga ttcggcggcg ggcgcgtgga gcgaggggta
     2281 ggctgatggg cggcccagcg ggatcagacg aaacggcgcg agtgcgcgag gtatatgccg
     2341 agcgcgccga gcgtttggcg ggggccggaa cctattcgct gaccaacgac gcctatctgt
     2401 tcgccatcca gagccggcag cgggcgttgt tgcgcatgtt gcgacgcgag gggatgtggc
     2461 cactggccgg caaggacatc ctggaagtgg gctgcggcaa tggcggcgtg ttgctggagc
     2521 ttctcggcta tggggccgat cccc
//
LOCUS       DAOMQG010000449         2542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16392_length_2542_cov_8.574990, whole genome shotgun sequence.
ACCESSION   DAOMQG010000449 DAOMQG010000000
VERSION     DAOMQG010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16392_length_2542_cov_8.574990"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLC06_15810"
     CDS             <1..841
                     /locus_tag="PLC06_15810"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HRO91965.1"
                     /translation="QPDSPLLPPAVRWLMVARTAETWSTLHTTAWSVSALSDWMVASG
                     ELEPDFAYDLRVNLVSRASGSFAPTDATASEEVTVPVGELVQDGTNFFDFQRGEGNGV
                     LYYTMRLNSAIAVDQLDPVNRGFTVERRYFDAACDPETTACEPIDSIPAGGRVRVELT
                     VVVGNDRTYVLVEDPIPAGTDAIDPNLLTSESGRGGSIVPAEGEFGHGYWGWWYFDHI
                     QYRDEKVVFLSQYLPAGTYQYSYFLQANIPGTYQVMPATAREDYFPEVFGRSEGMVFT
                     IGE"
     gene            1222..1710
                     /locus_tag="PLC06_15815"
     CDS             1222..1710
                     /locus_tag="PLC06_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO91966.1"
                     /translation="MEEQDLMREQANVLADRAYRHQQHGEIGHAIALYRRSIATYPTA
                     EAHTWLGWAYALMDRYDEAIAECEKAIAIDPDYGNPYNDVGAYLIESGRPEEAIPWLE
                     QALVASRYTTPQFAHVNIGRAWEQLGEPYQALVAYDRARAIAPLYLPATWAKTILLGR
                     LN"
     gene            1724..2203
                     /locus_tag="PLC06_15820"
     CDS             1724..2203
                     /locus_tag="PLC06_15820"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRO91967.1"
                     /translation="MPRAIGRIAVIAVGKLRERHWLSAQDEYARRLGHYTDFNLIEVK
                     DAVGKSLPDAVAMVREGEQLLAAVPRGARLVVLDPGGKSMSSPDLAVWLQAQLEAHGS
                     LTFLIGGPLGFDPAVTAAAHERLALSPLTFTHEMARIILMEQLYRAFTILHGEPYHK"
BASE COUNT          481 a          858 c          734 g          469 t
ORIGIN      
        1 gcagccggac agcccgctgc tgccgccggc ggtgcgctgg ctcatggtcg cccgcacggc
       61 cgagacctgg tcaacgctgc acacgacggc gtggagcgtg tcggccctca gcgactggat
      121 ggtcgcatcc ggcgagctgg agcctgactt cgcttatgat ctgcgggtca acctcgtttc
      181 gcgggcgtcg ggcagctttg cccccaccga cgcgaccgct tcagaagagg tgaccgtgcc
      241 ggtcggcgaa ctggtgcagg acggcacgaa tttcttcgac ttccagcgcg gcgagggcaa
      301 cggcgtgctc tattacacga tgcgcctcaa cagcgccatc gccgtcgacc aactcgaccc
      361 ggtcaatcgc ggcttcacgg tggagcgccg ctacttcgac gccgcctgcg acccggagac
      421 gacagcctgc gaacccatcg acagcatccc cgccggcggc cgcgtgcgcg tcgagctgac
      481 cgtcgtcgtg ggcaacgacc ggacctatgt cctcgtcgaa gaccctatcc ccgccggcac
      541 cgacgccatc gacccgaatc tgctgaccag cgagtcgggc cgcggcggca gcatcgtccc
      601 cgccgagggc gagttcggtc acggctactg gggctggtgg tatttcgacc atatccagta
      661 ccgcgacgag aaggtggtgt tcctgagcca atacctgccg gccggaacgt atcagtacag
      721 ctacttcctg caagccaaca tccccggcac ctatcaggtg atgccggcga ccgcccgcga
      781 ggattacttc cccgaggtgt tcggccgctc ggaagggatg gtgtttacga ttggggagtg
      841 acgaaggacg aaatacgaag gacgctccgc tcgtagaaac cgagtttctt ccgagaaact
      901 cggtttccag gcggcgctta attccgaaga aggggcgaga caaccggcga cagcgctgaa
      961 gcagttaact acaaacgggt tcgcaccggc cgcatgttgg tattgggcgg gtttgaaacc
     1021 cgcccctaca attaccgtgc cggttgtccg cccatataat tcagtataaa tactgcatat
     1081 tcggcagggt caggcgaccg ggcagcaacg ccgttcgttt cccggccacg cctttccctg
     1141 tcgccagacc cctacgaccc atcgtcactc gtcattcgtc actggacatc gccccgcccg
     1201 ccagctaaac tagtgcccat aatggaagaa caagacctca tgcgcgagca ggccaacgtc
     1261 ctggccgacc gcgcttaccg ccaccagcag cacggggaga tcggccacgc catcgccctc
     1321 taccggcgat ccatcgccac ctaccccacc gccgaggccc acacctggct cggctgggcc
     1381 tacgccctca tggatcgcta cgacgaagcc atcgccgaat gcgagaaggc gatcgccatt
     1441 gaccccgact acggcaaccc ctataacgac gttggcgctt atctgatcga atccgggcga
     1501 ccggaagagg ctatcccctg gctggagcag gcgcttgtgg cctcgcgcta cactacccct
     1561 cagtttgccc atgtcaacat cggccgcgcc tgggaacagc tgggtgagcc gtatcaggct
     1621 ctggttgcct acgaccgcgc ccgggccatc gccccactct atctgccggc tacctgggcc
     1681 aaaaccatcc tgctcggccg cctcaactag gcccgcgctt gccatgcccc gcgccatcgg
     1741 ccgaatcgct gtcatcgccg tgggcaagct gcgcgaacgc cactggttga gcgcgcagga
     1801 cgaatacgcc cggcggctcg gccactacac cgacttcaat ctgattgagg tgaaggatgc
     1861 ggtgggcaaa tcgctgcccg acgccgtggc gatggttcgc gagggagaac aactgctggc
     1921 ggccgttccg cgtggcgcgc gtctggtcgt gctcgacccc ggcggcaaat caatgagcag
     1981 cccagatctg gccgtctggc tgcaagcgca actggaagcc cacggcagcc tgaccttcct
     2041 catcggcggc ccgcttggct tcgacccggc agtgacggcc gccgcccacg agcgactggc
     2101 cctctccccc ctcaccttca cccacgagat ggcccgcatc atcctgatgg agcagctcta
     2161 ccgggcattc acgattctcc acggggagcc ataccataag taattaggaa ttaggaccac
     2221 ggacgacgga cgtcgcgact cccgtcgcgc gatcacagaa agagcgccct cttcgttcgt
     2281 acttcatcgt tcgtactatg gataccttcc tgtcgtggtc acacgacagg agtcgcggta
     2341 gtctgtgatc tgtggtctgt gatctctttc gtacttcata cttctcgtca caaaaaccgg
     2401 gtttctcgga agaaacccga ttttttaatc gacgatccgc aaagacccct tgcccaaggg
     2461 gtcttttgca ttgtatgctt cgtcagttgc caaggagaga tgatgattaa tcggctgttt
     2521 gtctatggta cgcttgcgcc cg
//
LOCUS       DAOMQG010000450         2541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16400_length_2541_cov_6.337490, whole genome shotgun sequence.
ACCESSION   DAOMQG010000450 DAOMQG010000000
VERSION     DAOMQG010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16400_length_2541_cov_6.337490"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PLC06_15825"
     CDS             <1..932
                     /locus_tag="PLC06_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRO91968.1"
                     /translation="SAQTYYTIRCLADRDRVADAYRAYAYFRWVDDHIDEGDAGAAER
                     AAFLRRQQMLLEAVYRGRMPGDLCLEEQLLVDLINGDAEPDSGLHAYLDNMMAVMAFD
                     LERRGRLISAAELERYTDLLATAVSEALFHFIGHDTPPPLEGRATAVRGAHIIHMLRD
                     LIEDCAGGYYNIPAEYLAAHGIGMGDCDHPAFRAWVAERVALARDCFREGRAFIARVP
                     SARCRLAGRAYIARFEYVAGLIERDGYRLRAAYPERKSARAALWMGGGRQLRITNYEL
                     RIRKMTADNRQQTTVVVGRQELSCRPPNNRMIT"
     gene            929..2428
                     /gene="crtI"
                     /locus_tag="PLC06_15830"
     CDS             929..2428
                     /gene="crtI"
                     /locus_tag="PLC06_15830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRO91969.1"
                     /translation="MNKSAIVIGAGMGGIVAAAHLARHGLRVTVIEKNARPGGRCDRI
                     SREGHQFDTGPTLMVMPLLYEAEFAALGADMRELVDLRRIDPTYHLVFDDGSQLSLTS
                     NMDDMREQLEAIEPGAFAAFRRYLDEGRRHYEIGTEKLVYRDFRTFGDFFNIDNLPLI
                     YQLKPFARHYDHMSTYFDSDRLKAAFTFQDVYMGLSPFEAPATFSMMPYTEIAHGVWY
                     PRGGMYAIVEAMMGLARDAGVEFVFDTEVTRIIIADDRARGVVLEDGDQVWADVVVAN
                     ADLPYVYDELLPPSEQAERLARKRYSCSTISFFWGVDCECVMPPHTLFLAEDYRENFV
                     SIIRDLAMPANPSVYIHAPARLDPTMAPPGEDTLIAVVPVGHLSDTKAQDWGEIRDRA
                     RDHVFRRLALLGITDLQDHIKFETSFNPLSWRKRYNLVKGSTHGLCHNLTQLAYFRPG
                     NRHPDYENLYFVGASTHPGTGLPTAMVSGRLVARRVMDELGVYAEAAVA"
BASE COUNT          436 a          806 c          831 g          468 t
ORIGIN      
        1 ccagcgccca gacctactat accatccgct gcctggccga ccgcgaccgc gtcgctgacg
       61 cctatcgcgc ctatgcctac ttccgctggg tcgatgacca cattgatgag ggcgacgccg
      121 gcgcggccga gcgggccgcc tttctgcggc ggcagcagat gctgctggag gcggtctatc
      181 gcggccgaat gccgggcgat ctttgtctcg aagagcaatt gttggtcgat ctgatcaatg
      241 gggacgcgga gccggatagc ggcttgcacg cctatctgga caacatgatg gcggtgatgg
      301 ccttcgacct ggagcggcgc gggcggctta tttcggccgc cgaactggag cgctataccg
      361 acctgctggc gacggccgtc tcggaagcct tgttccattt catcggccac gacacgccgc
      421 cgccgctgga agggcgcgcg acggccgtgc gcggcgctca tatcatccac atgctgcgcg
      481 acctgatcga ggattgcgcc ggcggctact acaacatccc cgccgaatat ctggccgcac
      541 acggtatcgg catgggcgat tgcgaccacc cggcctttcg ggcgtgggtg gccgagcgcg
      601 tggcgctggc ccgcgactgc ttccgcgagg ggcgagcgtt catcgcgcgc gtgccgagtg
      661 cccgctgccg gttggccggc cgggcttata tcgcccgctt tgaatacgtg gccggcctca
      721 tcgagcgcga cggctatcgc ctgcgggccg cttacccgga gcgcaagtcg gcgcgggcgg
      781 cgctgtggat ggggggggga agacaattac gaattacgaa ttacgaatta cgaatcagga
      841 agatgactgc ggacaacaga caacagacga cggtggtggt cgggcgacag gagttgtctt
      901 gtcgtccgcc aaacaatagg atgatcacat gaataagtca gctatcgtca tcggcgcggg
      961 gatgggcggg atcgtggcgg cagcgcatct ggcccggcac ggcttgcggg tgacggtcat
     1021 tgagaagaac gcgcggcccg gcggccgctg cgaccgcatc agtcgcgagg ggcaccagtt
     1081 cgacaccggc ccgacgctga tggtcatgcc gctgctctat gaggcggagt tcgccgcgct
     1141 gggagccgac atgcgcgaac tggtggacct gcggcgcatt gacccgacct atcatctggt
     1201 gtttgacgac ggcagccagc tctcgctgac gtcgaatatg gacgacatgc gcgagcagct
     1261 ggaggccatc gagccgggcg cgttcgccgc cttccggcgc tatctggatg agggccgtcg
     1321 ccattacgag atcggcaccg agaagctggt ctatcgcgac ttccgcacct tcggcgattt
     1381 cttcaacatc gacaacctgc ccctcatcta tcagttgaag ccctttgccc ggcactatga
     1441 ccacatgtcc acctacttcg acagcgaccg gctgaaggcg gccttcacgt ttcaggatgt
     1501 ctacatgggc ctcagcccat tcgaggcccc ggccacattt tcgatgatgc cctacaccga
     1561 gatcgcccac ggcgtctggt atccgcgggg cggcatgtat gccatcgtcg aggccatgat
     1621 ggggctggcg cgcgatgccg gggtggagtt cgtcttcgac acggaggtga cgcggatcat
     1681 catcgctgat gatcgggcac ggggcgtcgt gctggaggac ggcgatcagg tgtgggccga
     1741 tgtggtggtg gccaacgccg atctgcccta cgtctacgac gaattgttgc cgccgagcga
     1801 gcaggcggag aggctggccc gcaagcgcta ctcctgctcg acgatcagct tcttctgggg
     1861 cgtcgattgt gaatgcgtca tgccgcccca cacgctgttc ctggccgagg attaccgcga
     1921 gaacttcgtc agcatcatcc gcgatctggc catgcccgcc aatcccagcg tctacatcca
     1981 tgccccggcg cggctcgacc cgacgatggc cccgccgggc gaggacacgt tgatcgccgt
     2041 cgtgcccgtc ggccacctct ccgacaccaa ggcacaggac tggggcgaga ttcgcgaccg
     2101 cgcgcgcgac cacgtctttc ggcggttggc gctgctgggc atcaccgacc tgcaagacca
     2161 tatcaagttt gagacgtcgt tcaacccgct gtcgtggcgc aagcggtata acctcgtcaa
     2221 gggttcgacc catgggctgt gccataacct gacccaactg gcttactttc ggccgggcaa
     2281 tcgtcacccc gactacgaga acctctattt tgtgggggcc agcacccacc ccggcaccgg
     2341 tctccccacg gcgatggtgt ccgggcggct ggtggcgcgg cgggtgatgg atgagctggg
     2401 cgtttacgcg gaagcggccg tcgcttgatc tgtggcccgg cgggcaagcc gcgaacatat
     2461 tttgcgcggg gtataatcgc gcgcccgata cagtagcaat atccccacgg gcgctctata
     2521 ctcaccgctc aagatggact c
//
LOCUS       DAOMQG010000451         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16427_length_2537_cov_10.567284, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000451 DAOMQG010000000
VERSION     DAOMQG010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16427_length_2537_cov_10.567284"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1450
                     /locus_tag="PLC06_15835"
     CDS             242..1450
                     /locus_tag="PLC06_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO91970.1"
                     /translation="MDFSFSDQHKMIERMVYDFSRKEIMPIIKDHDRNHTFPLELLPK
                     MGELGFLGICLPVRYGGAGMDYLSLAILSEGLEFADSSIRETIAVHLGLHALPIFQWG
                     TDEQRRDFLPPLATGEHIACFGLTEPGAGSDVAAMGSRARRDGDDYILNGEKMWITLA
                     DVADRFLVFAKTDPDKGAGGISAFILERGWPGLTTGTIEGKMGVRASNTGWINMADVR
                     VPASHRLGEEGEGFKIAMSALDNARYGVAAGAVGIVKACLEESIAYGRQRHTFGRPIV
                     DYQLVQQMLAHMQQSIDAGQLLVWKAGWLKNKGIRNTRETSAAKWYCTEAARRAADDA
                     VQIHGAYGYSDEYNVERHLRNTKSAVIYEGTSQIHTLMQAGYALGERHDRPMRRELPA
                     YDPEYWEAES"
     gene            1551..2129
                     /locus_tag="PLC06_15840"
     CDS             1551..2129
                     /locus_tag="PLC06_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRO91971.1"
                     /translation="MTAIYTIGYGARDIDAFMAALRRYNIAYLIDVRSQPYSRYKPDF
                     SKDALEEHLRRGGIRYVYMGDTLGGRPDDPTCYDAEGKVDYTAVTQRDFYQAGIERLT
                     KAWSQGLTVSLMCSEGKPEMCHRSKLIGRTLTERGLTVTHIDEHDEAISQAEVILRLT
                     GGQLSLFEDTRPGSTSRKSYGPVGREEEDEYE"
     gene            2122..>2537
                     /locus_tag="PLC06_15845"
     CDS             2122..>2537
                     /locus_tag="PLC06_15845"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRO91972.1"
                     /translation="MNKIVTIGAIGYTADAFFSALQEAGVDTFVDVRRRRAVRGAAYA
                     FANSQRLQARLAELGIRYLHRLDLAPPTTVRQQQAAADAAAGVAKRKRAELSPTFVTL
                     YRSEVLASFDPQSLHDDLPPDAAVIALMCVEREPAA"
BASE COUNT          486 a          801 c          794 g          456 t
ORIGIN      
        1 atcctggccg gagcgctgct gctggcgggc gtggccgatc ggtttgaatg ggcagagttg
       61 cgagccgtgg ttgtcgcgat tgcgctgctc gtgttgctgt tctgcgcggt ggccgtgatc
      121 cggctgccaa tggcctgacc tgcctgtcag ggttgggcgt ggtgacgtat aatcccttcc
      181 gtgccggggc catcactccg attggcccgc ggcgactatc actgttttca aggaatgacc
      241 gatggatttc tcttttagcg accaacacaa gatgatcgag cgcatggtct atgatttttc
      301 gcgcaaggag atcatgccga ttatcaaaga ccacgaccgc aaccatacct tcccgctgga
      361 gcttctgccc aagatgggcg agttgggctt cctgggcatc tgcttgccgg tgcgctacgg
      421 cggcgcgggc atggactatc tctcgctggc gatcctgtcc gaggggctgg agttcgccga
      481 ctcgtcgatt cgggagacga tcgccgtcca cctggggctg cacgcactgc ccattttcca
      541 gtggggcacg gacgagcagc ggcgcgattt tctgccgccg ctggccacgg gcgagcatat
      601 cgcctgcttc ggtctgacgg agccgggggc cgggtccgac gtggcggcca tgggcagccg
      661 cgcccggcgc gacggcgacg actatatatt aaacggcgag aagatgtgga tcaccctggc
      721 cgacgtggcc gaccgcttcc tcgtcttcgc caagactgat cccgacaagg gcgcgggcgg
      781 catcagcgcc tttatcctgg agcgcggctg gccggggctg accaccggca ccatcgaggg
      841 caagatgggc gtacgagcca gcaacacggg ctggatcaat atggccgacg tgcgcgtgcc
      901 ggcaagccac cggctgggcg aggaaggcga agggttcaag atcgccatga gcgcactcga
      961 caacgctcgc tatggcgtgg cggcgggagc tgtcggcatc gtcaaggcct gcctggaaga
     1021 gtcaatcgcc tacggccgcc agcgccatac gttcggccgt cctatcgtcg attaccagct
     1081 ggtgcagcag atgttggccc acatgcagca gagcatcgac gccgggcagc tgctggtgtg
     1141 gaaggcgggc tggctgaaga acaagggcat ccgcaacacg cgggagacga gcgcggccaa
     1201 atggtattgc accgaagccg cccgacgcgc cgccgacgac gcggtgcaga tccacggcgc
     1261 ctatggctac tctgacgagt acaatgtgga gcgccatctg cgcaacacca agagcgccgt
     1321 catctacgaa gggacgtcgc agatccacac attgatgcaa gcggggtatg ccctgggcga
     1381 gcgtcacgat cggccgatgc gccgcgaact gcccgcctat gatccggagt attgggaagc
     1441 tgaaagttag gccaggactg tggcgcaaac atctctgatt tcgcccccga acagagatgt
     1501 ttgcaccata aaacgattgt ttctctcccg ccatcctcta caattgggcc atgaccgcaa
     1561 tttacacgat cggctacggc gcgcgcgaca tcgatgcctt catggccgcc ttgcgccggt
     1621 acaacatcgc ctatctgatc gacgtccgct cgcaacctta ttcccgctac aagcccgact
     1681 tctccaaaga cgccctcgag gaacacctgc gccggggcgg aatacgttat gtctatatgg
     1741 gcgacacgct cggcggccgc cctgacgacc caacctgcta cgatgccgag ggcaaagtcg
     1801 attacaccgc cgtgacccaa cgagacttct atcaggcggg catcgaacgc ctgacgaaag
     1861 cctggtcgca aggtctaacg gttagcctga tgtgcagcga gggcaagccg gaaatgtgtc
     1921 atcgcagcaa gctcatcggc cgtacgttga ccgagcgtgg cctcacagta actcatatcg
     1981 acgaacatga tgaggcgatc agtcaggccg aggtgatact tcgtctgacc ggcgggcaac
     2041 tctcgctctt tgaggacacc aggccgggca gcacctcgcg caaaagctac ggcccggtcg
     2101 gccgcgagga agaggatgag tatgaataaa atcgtcacca tcggcgccat cggctacacg
     2161 gccgacgctt ttttcagcgc cctgcaggaa gccggagtcg ataccttcgt cgatgtgcgc
     2221 cgccggcgag ccgttcgcgg cgcggcctat gcctttgcca acagccaacg gctgcaggcc
     2281 cggctggccg aattgggcat ccgctatctc catcgactcg acctggctcc gccaaccact
     2341 gttcgccaac agcaagccgc ggccgatgcg gccgccggtg tggccaagcg caagcgcgcc
     2401 gaactcagcc cgacattcgt gacgctctat cggagcgagg tactggcctc tttcgacccg
     2461 caatcgctgc acgatgacct gccacccgac gcggccgtca tcgccctgat gtgcgtcgag
     2521 cgcgagcccg ccgcctg
//
LOCUS       DAOMQG010000452         2514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16625_length_2514_cov_9.447336, whole genome shotgun sequence.
ACCESSION   DAOMQG010000452 DAOMQG010000000
VERSION     DAOMQG010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16625_length_2514_cov_9.447336"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..453)
                     /locus_tag="PLC06_15850"
     CDS             complement(142..453)
                     /locus_tag="PLC06_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019494960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HRO91973.1"
                     /translation="MKSLAQIRVQLANGSYELSRHAFRRIVERNIGELEIREAGHNVV
                     IIEDYPADKYAPSCLLLGYTVAVRPLHIQVSRADNSTLRIITIYEPDLTEWEAGFTVR
                     R"
     gene            complement(465..2495)
                     /locus_tag="PLC06_15855"
     CDS             complement(465..2495)
                     /locus_tag="PLC06_15855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRO91974.1"
                     /translation="MTEPQTPDAPRLTTTDDLYRYHVPADPQISPDGRRVAWIVQRVE
                     RETEKKYTNLWLADVDTGRLRQFTHGKWNDSRPRWSPDGETIALVSNRGSEEQAQIYL
                     LPLGGGEARPLTELKGSIADYEWSPDGTQLVCQFRKKDAGALEREKDEGKKKLGISYR
                     HITRTDYKFDGMGYLPQEHWHIWVIDAATGAATQLTDGDKDETEPRWSPDGKQILFNS
                     NRSDRPDMDLDVTELYLIPAEGGEMRQLETRPGRKYGAVFSPDGQYVAWLGRDKPGRW
                     YQNARLYVAPVAGGEVRDISAAIDLHLGSSTNGDIFNGSGPYQLVWSADGRRIYSGAS
                     WHGDEPLVALDVNEPRGDYTRVIGEPGVVGGYTFDRERKRVAWLWSSLHDPVQLRVCD
                     IDAGGAAGEPRTLTALNPWHDEIERGTIEEVWFKARDGYDLHGWILKPPGFDPSQKYP
                     SILEIHGGPQTQYGNMFMHEFHYLAAAGYVVYWSNPRGSQGYGEGMTGAIFGRWGTVD
                     YDDVMDWTDYVEKLPWIDTERMGVTGGSYGGYMTTLIIGRTHRYKAAVAQRVVSNFIS
                     FYGSSDLNAGLENLAGTPPPWEDMTTNWAQSPISYIGSAKTPTLVVHSENDYRCDQEQ
                     GEQVFISLRKLGVDTELLLLPGESHGVSRGGRTDRRVARLEHMLGWFDKYLK"
BASE COUNT          394 a          775 c          842 g          503 t
ORIGIN      
        1 aggacatctc gccgcattgc gcgcaaaccc gaacagggat gttctcaacc aatacccggc
       61 ggccgtcgat ttcaaaaatc tccgagatcg tatcgatccg gctttcagaa ctgccgcaaa
      121 cctcgcaagc gaccatggga attatctcct cactgtaaaa cccgcttccc attctgttaa
      181 gtcgggttca taaatcgtta ttatcctcaa ggtcgagtta tccgcgcggg acacttgaat
      241 gtgcaaaggc cgaacagcca cggtgtaccc caggagcaga cagcttgggg cgtacttgtc
      301 cgcgggataa tcttcgatga tgacgacatt atggcctgct tcgcgtattt ccagctcgcc
      361 gatatttcgc tcaacgatac ggcgaaatgc atggcgggac aattcgtagc tgccattcgc
      421 caattgtacg cgaatttggg ccagagattt catgaagtta atcgtcactt caaatatttg
      481 tcgaaccagc ccagcatatg ctccagccgg gcgacgcggc ggtcggtgcg gccgccgcga
      541 gatacgccgt gggattcgcc gggcagcagc agcagctccg tgtctacgcc cagcttgcgc
      601 agggagatga acacctgctc gccctgctcc tggtcgcagc ggtagtcgtt ctcgctgtgg
      661 acgaccagcg tcggcgtttt ggcgctgccg atgtagctga tgggcgattg ggcccagttg
      721 gtcgtcatgt cttcccacgg cgggggcgtg ccggccaggt tctccagccc ggcgttgaga
      781 tcgctggagc cgtagaagct gatgaagttg ctgacgacgc gctgggccac ggcggccttg
      841 tagcggtggg tgcggccgat gatgagcgtg gtcatgtagc cgccgtagct gccgccggtg
      901 acgcccatgc gttcggtgtc gatccagggc agtttctcga cgtagtcggt ccagtccatc
      961 acgtcgtcgt aatcgaccgt gccccagcgg ccgaagatgg ccccggtcat gccctcgccg
     1021 tagccctggc tgccgcgcgg gttgctccag tagaccacgt agccggccgc ggccaggtag
     1081 tggaactcgt gcatgaacat gttgccgtat tgcgtctgcg gcccgccgtg gatctccagg
     1141 atggacgggt atttttgcga cgggtcgaag ccgggcggct tgaggatcca gccgtggaga
     1201 tcgtaaccgt cgcgggcctt gaaccagacc tcttcgatgg tgccgcgctc gatctcgtcg
     1261 tgccaggggt tgagcgcggt cagcgtgcgc ggttcgccgg ccgcgccgcc ggcgtcgatg
     1321 tcgcacacgc gcagttgaac cggatcgtgg agcgacgacc acaaccaggc cacgcgcttc
     1381 cgctcgcggt cgaaggtgta gccgccgacg acgcccggct cgccgatgac ccgcgtgtaa
     1441 tcgcctcgcg gctcattgac gtccagcgcg acgagcggct catcgccatg ccacgacgcg
     1501 ccgctgtaga ttcgccggcc gtcggccgac cagaccagct ggtatggccc tgagccgttg
     1561 aagatgtcgc cgttggtgga cgagccgagg tgcaggtcga tcgccgcgct gatgtcgcgc
     1621 acttcgccgc cggcgacggg ggccacgtag agccgcgcgt tctgatacca gcggccgggc
     1681 ttgtcgcggc ccaaccaggc cacgtattgc ccgtcgggcg agaagaccgc gccgtacttg
     1741 cggccgggcc gggtctccag ttggcgcatc tcgccccctt cggcggggat gagatacagc
     1801 tcggtcacgt ccagatccat gtccggccgg tcgctgcggt tggagttgaa caggatctgc
     1861 ttgccgtcgg gcgaccagcg cggctcggtc tcgtccttgt cgccgtcggt cagctgggtg
     1921 gccgcgccgg tggcggcgtc gatgacccaa atgtgccaat gctcctgggg cagatagccc
     1981 atgccgtcga atttgtagtc ggtgcgggtg atatggcggt aggagatgcc gagctttttc
     2041 ttgccttcgt ccttctcgcg ctccagcgcg ccggcgtctt tcttgcggaa ctggcagacg
     2101 agctgcgtgc cgtcgggcga ccattcgtaa tcggcgatgc tgcccttgag ttcggtcagg
     2161 gggcgggcct cgccgccgcc gaggggcagc aggtagatct gcgcctgctc ctcgctgccg
     2221 cgattggaca cgagggcgat cgtctcgccg tcgggcgacc agcgcggccg gctgtcgttc
     2281 catttgccgt gggtgaactg gcgcaggcgg ccggtgtcaa cgtcggccag ccacaggttg
     2341 gtgtatttct tctccgtctc gcgctccacg cgctggacga tccaggccac ccggcggccg
     2401 tcgggcgaga tctgcgggtc ggcggggacg tggtagcgat acagatcgtc ggtcgtggtc
     2461 aggcggggcg cgtcgggcgt ttggggttcg gtcatggggt caactccagg gtta
//
LOCUS       DAOMQG010000453         2502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16723_length_2502_cov_8.917450, whole genome shotgun sequence.
ACCESSION   DAOMQG010000453 DAOMQG010000000
VERSION     DAOMQG010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16723_length_2502_cov_8.917450"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLC06_15860"
     CDS             complement(<1..440)
                     /locus_tag="PLC06_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91975.1"
                     /translation="MGADDEFIVDGDENGANGSSHTEDANNRTFLILASLLTGTLLLI
                     SLVAVGFILMNRSSERAQTVAAIETQNAVILATNAAVTMTIEAAQVNAAAPEQAIAES
                     DIAPTDIAVKGGISPEDATATADALATIAADPTLVAAVTAAAGGA"
     gene            588..1493
                     /locus_tag="PLC06_15865"
     CDS             588..1493
                     /locus_tag="PLC06_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRO91976.1"
                     /translation="MRVKVIINSRSGRGRGREQTDLISSLSRRYSQLEVFPTERPRHA
                     TELAATAADEGYDLVVAAGGDGTVHEVVNGLMRGDQAALPLGIIPIGSGNDLAFGLGI
                     PEEVEGAVERIFKGTPQPLDLARVTNEYGKTEVFDNNFGIGFDAIVVMRTETITRLHG
                     FPMYMAAVLQTILFYYNIPYLEATFDGQPVNQRSLFLYMGLGQRGGGGFLLTPDARWD
                     DGLIDTMLVNPIGRLTMLGMLTSAMKGTHVTSPHVTFRQCREITVKSSAPMPIQVDGE
                     IFAYPRDDVHQVTITSLPAALRVIV"
     gene            1510..>2502
                     /locus_tag="PLC06_15870"
     CDS             1510..>2502
                     /locus_tag="PLC06_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91977.1"
                     /translation="MRNLEQALLDHELIVLRVIGEWLGLDLTGTDKATAVDQLTMALA
                     QVDLVNEMEYLEPEEAAALTDLVRQGGRAPVAVFARQHGEVRPMGPGRMEREEPWLDP
                     ANPAESLWYRGFLYRAFDQTDEGMLEFYYIPQELLYRLAPPAPVRREEPALEPDEPAL
                     GPTNAPVESRPTPTDAVDDLTTLLALAQRTGLRPDRLPDLDGLLANPDRDRRSMLLTL
                     ATEMSLLRRADDILRPTRAAIDWLQQSREAQLRALVDAWSRSAWNELRHTPGLTIEGE
                     GWQNDPLPARTALMDTLPADDHWYRLADVVAVIHAQDPDFQRPDGNYETWYIRDA"
BASE COUNT          461 a          832 c          744 g          465 t
ORIGIN      
        1 gcgccgccgg cagccgcggt aacggccgcc acgagcgtcg gatcggcagc gatcgtggcc
       61 agtgcgtcgg ccgtcgctgt ggcatcttcc ggcgatatgc cgcccttcac ggcaatatca
      121 gtgggggcga tgtcgctctc ggcaatcgct tgttcgggcg cagcagcatt gacctgagcc
      181 gcttcaattg tcatggtcac ggcggcattg gtggccagaa tgacggcgtt ctgtgtttca
      241 atcgctgcta ccgtctgcgc gcgctcgctg gagcggttca tcaatataaa gccgacggcc
      301 accagcgaga tcaacaggag cgtgccggtc agtaacgagg ccaggatcag gaaggtcctg
      361 ttgttggcgt cttcggtgtg agagcttccg ttagccccgt tttcgtcacc gtcgacgata
      421 aattcatcat ccgcgcccat gatattctcc tcttcgctcc accggccaat agcctgtgat
      481 cggctgccat atgcgccatg tttacgcctt gtcagatggc tcaacttgac gtaacatcac
      541 gaacgcggtc agtataacat gtttgacaag gggtgtaaag cgaacctatg cgcgtcaagg
      601 tcatcatcaa ttcgcgttcg gggcgcggca gaggtcgcga acaaaccgat ctcatcagtt
      661 cactcagccg tcgctacagc cagctggagg tctttcccac cgaacggccg cgccacgcga
      721 cagaactggc cgcaaccgcg gccgatgagg gttacgatct ggtcgtcgcg gccggcggcg
      781 acggcacggt tcacgaggtc gtcaacggcc tgatgcgcgg cgaccaggcc gccctgcccc
      841 tcggcatcat ccccatcggg tcgggcaacg acctcgcctt cgggctgggc atccccgaag
      901 aggtcgaggg agcggtcgaa cgcatcttca agggcactcc ccagccgctt gacctggccc
      961 gcgtgaccaa cgagtacggc aagaccgaag tcttcgacaa taacttcggc atcggcttcg
     1021 acgccatcgt cgtcatgcgc accgagacca tcacccggct gcacggcttt ccgatgtaca
     1081 tggctgccgt gctgcagacg atcctctttt actacaacat cccctatctt gaagccacct
     1141 tcgacggcca gccggtgaac cagcgctcgc tcttcctgta tatgggtctg ggacagcgcg
     1201 gtggtggcgg cttcctgctg acgcccgacg cgcgatggga tgacggcctg atcgacacta
     1261 tgctggtgaa ccccatcggc cgcctgacca tgctcgggat gctgaccagc gccatgaaag
     1321 gcactcacgt cacctctcct catgttactt tccgccaatg ccgcgagatc accgtcaaat
     1381 cgtccgcgcc catgcccatt caggtcgacg gcgagatatt cgcctatccc cgcgacgatg
     1441 ttcatcaagt caccatcacc agcctgcccg cagctcttcg ggtgattgtt tgacatcctg
     1501 aggtcatcta tgcgcaacct ggaacaggcc ctgctggatc acgaactcat cgtcctgcga
     1561 gtcatcggcg aatggctcgg cctcgacctc accggcacgg ataaagccac ggccgtcgat
     1621 cagctaacca tggctctggc ccaggtcgac ctcgttaacg agatggagta cctggagccg
     1681 gaagaggcgg ccgcgctgac cgatttggtg cggcaaggcg gtcgggcgcc ggtggctgtc
     1741 ttcgctcgcc agcacggtga agtcaggcct atggggccgg gcaggatgga gcgcgaagaa
     1801 ccgtggctcg acccggctaa cccggccgaa tccctctggt accgcgggtt cctctatcgc
     1861 gcgttcgacc agacggatga gggcatgctg gagttctact atatccccca ggaactgctc
     1921 tatcgcctcg cgcccccggc tccagttcga cgcgaggaac ccgccctcga acccgacgaa
     1981 ccggccctgg ggcctacgaa tgcgcccgtc gaatcgcgcc cgacgccaac cgacgcggtc
     2041 gacgacctaa ccacactgct ggcattggcc cagcgcaccg ggttacgccc cgatcgattg
     2101 cccgacctcg acggcttgct ggccaacccc gaccgcgacc ggcgctcgat gctcctgacc
     2161 ctggcgacgg agatgtcgct cttgcgccgg gccgatgata tcctgcggcc gacgcgcgcg
     2221 gccatcgact ggctgcaaca aagccgcgag gcacaactgc gcgcgctggt ggatgcctgg
     2281 agccgcagcg cgtggaatga attgcgtcac acgccgggcc tgacaatcga gggcgagggt
     2341 tggcaaaatg accctcttcc ggcccggacg gcgctgatgg acacgctgcc ggccgatgac
     2401 cattggtacc ggttggccga tgtggtagct gtcattcacg cccaggaccc cgattttcag
     2461 cgccccgacg gcaactacga gacctggtac atccgcgatg cc
//
LOCUS       DAOMQG010000454         2501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16732_length_2501_cov_16.322159, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000454 DAOMQG010000000
VERSION     DAOMQG010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16732_length_2501_cov_16.322159"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLC06_15875"
     CDS             <1..855
                     /locus_tag="PLC06_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRO91978.1"
                     /translation="SKALPGKHQEIRGLEHLDKVIEIDQSPIGRTPRSNPATYTNLFN
                     QIRDLFSSLPESKLRGYQPGRFSFNVKGGRCEACQGQGQNRIEMQFLPDIYVPCDICK
                     GARYNRETLQVKYRDLNIAEVLDLTVDEALEFFTNFPPIKRKLQTMADVGLGYIHLGQ
                     PATTMSGGEAQRVKLSRELSKIATGRTLYILDEPSVGLHAHDVSKLIIALNKLVDKGN
                     TVVIIEHNLDIIKVADHIIDMGPEGGDRGGEIIAQGTPEEIARVEASYTGEYLRPLLN
                     GHVVAPNS"
     gene            complement(852..1268)
                     /locus_tag="PLC06_15880"
     CDS             complement(852..1268)
                     /locus_tag="PLC06_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91979.1"
                     /translation="MIQANSNIDGNRILTVQPNGTKVYTPFPDYEETVPPSGGATKRV
                     AFSLAGQLIAVRVITDTTNNYYYAYADHLGNISAWTNASGALVSGSLARYEPFGGYRT
                     KPASTVNPGISDRGFTGHRQNNTGNNDPGLIYMNAR"
     gene            complement(1265..1792)
                     /locus_tag="PLC06_15885"
     CDS             complement(1265..1792)
                     /locus_tag="PLC06_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91980.1"
                     /translation="MKQLVDTQLAALGSQWSQRGRGSLNTDSDFTSGMWAVWLGFDHT
                     DYRYNADEEIHVFYNASRAERNFSPSPMAVNIDKDGNPPIGWTYSPPHADRFVIDCSS
                     EPVPKTCYLLLRYQEYVIVYATDVAGAMTLKDLQKLIEVTDSFMHKFLSETRLEPNVR
                     EIPTLSELGLSQEGD"
     gene            complement(1924..>2501)
                     /locus_tag="PLC06_15890"
     CDS             complement(1924..>2501)
                     /locus_tag="PLC06_15890"
                     /inference="COORDINATES: protein motif:HMM:NF026945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 44 domain-containing
                     protein"
                     /protein_id="HRO91981.1"
                     /translation="ANVIHAGNIIADIPFATGTSRAMGYLIANAVWDSQVMDARLKDH
                     VGVFAPYIANWDHKPILRGRNGPSPVREIVERGNSSRVGAELYRFDIWSNIHYGYVGV
                     ASGFSESELINGAGAEQIGSVLYSGGQPSSSPNSPTWLSSWDEPSDTASIQIGSALWN
                     NYGLNVTPADLYLAVIDETRLCSTQCLGAGQ"
BASE COUNT          569 a          682 c          699 g          551 t
ORIGIN      
        1 tcgaaggccc tgcccggcaa gcatcaggag attcgcgggc tggagcatct cgacaaggtg
       61 atcgagatcg accaaagccc gatcggccgc acgccgcgca gcaacccggc cacatacacg
      121 aacctgttta accagattcg cgacctgttc agcagcttgc cggagagcaa gttgcgtggc
      181 tatcagccgg gccgtttcag cttcaacgtc aagggcggcc gctgcgaggc gtgccaggga
      241 caggggcaga accgcatcga gatgcagttc ctgcccgaca tctacgtgcc ctgcgacatc
      301 tgcaagggcg cgcgctataa ccgggaaacg ttgcaggtga aatatcggga cctcaacatt
      361 gccgaggtgc tcgacctgac cgtggatgag gcgctggagt tcttcaccaa cttcccgccg
      421 atcaagcgca agttgcagac gatggccgac gtgggtctgg gatacatcca cttgggccag
      481 ccggccacga cgatgagcgg cggcgaggcg caacgggtga aactgagccg cgagctgtcg
      541 aagatcgcca ccggccgcac gctctacatc ctcgacgagc cgtcggtcgg cctgcacgcc
      601 cacgacgtca gcaagctcat catcgcgctg aacaagctgg tcgacaaggg caacacggtc
      661 gtcatcatcg agcataatct cgacatcatc aaggtcgccg atcatatcat cgacatgggg
      721 ccggaaggcg gcgaccgcgg cggcgagatc atcgcccagg gcacgccgga agagatcgcg
      781 cgggtcgagg cgtcctatac cggagaatac ctgcggccgc tcctgaatgg gcacgtcgta
      841 gcgccgaatt cctagcgggc attcatgtag atgaggcccg ggtcattatt gccggtgttg
      901 ttttgccggt ggccggtgaa gccgcggtcg ctgatgccgg ggttgactgt tgaggccggc
      961 ttggtgcggt agccgccgaa cggctcataa cgggccagtg agccgctgac caatgcgcca
     1021 ctcgcattcg tccaggcgct gatgttgccc aggtggtcgg cgtaggcgta gtaataatta
     1081 ttcgtggtat cggtgatgac gcgcacggcg atgagttgtc cggccagaga gaaagccacc
     1141 cgtttggtgg ctccgccact cggcggtact gtctcctcgt agtcggggaa gggcgtgtag
     1201 accttcgtcc cgttgggttg tacggtcagg atgcggttgc cgtcgatgtt gctattggcc
     1261 tggatcaatc accttcctga gataatccga gttcactcag ggtcgggatt tcacgtacat
     1321 tcggctctaa acgagtttcg gagagaaatt tgtgcataaa gctatccgtc acttcaataa
     1381 gcttctgtaa atctttaagc gtcattgctc cagctacatc ggtagcatac acaatgacgt
     1441 attcttggta tcttagcaag agatagcagg tctttggtac aggttcacta ctacaatcga
     1501 tgacaaatcg atcggcatga ggcggagagt aagtccagcc tatgggcgga tttccatcct
     1561 tgtcgatgtt aactgccatc ggcgagggag aaaagtttct ttcggcgcgt gatgcgttat
     1621 aaaagacatg gatttcctcg tctgcattat aacgataatc ggtgtggtcg aatcctagcc
     1681 aaacagccca catacccgat gtaaaatctg aatctgtatt gagactgccc ctacctcgtt
     1741 gtgaccattg agaccccaac gctgccagtt gtgtatcaac taattgtttc atcgggatat
     1801 cataggggac tcgccttgcg ggctcgagcc agttagtgac tcgcttaaca atcggaatag
     1861 ttgctactag aatcacaatg cagccaacgc cacaggatac tatcaatcga cgtgcaccgt
     1921 tactcattga cctgctccta aacactgtgt gctacacagg cgtgtttcgt cgataacggc
     1981 caggtataaa tcggcaggag tcacatttag accgtaatta ttccatagcg cacttcctat
     2041 ttgaatggag gctgtatccg acggctcgtc ccaagaagaa agccaggtcg gggaattagg
     2101 cgaggaggat ggttggccgc cactatacag aactgaaccg atttgttccg cgccggcccc
     2161 gttgatcaat tcactttcgg agaagcctga tgcaaccccg acatacccat aatgaatatt
     2221 cgaccaaatg tcaaaccgat aaagttcggc tccgactctt gacgaatttc ctcgttcgac
     2281 aatttcccgt acaggtgaag ggccgtttct acctcgtaag ataggcttat ggtcccaatt
     2341 tgctatataa ggtgcgaaca cccccacatg atccttcaat ctggcgtcca taacctgcga
     2401 gtcccacacg gcgttagcaa tgagatagcc cattgccctg cttgtgcctg tggcgaaggg
     2461 aatgtcggct atgatattcc cggcatgaat cacatttgcc g
//
LOCUS       DAOMQG010000455         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16746_length_2499_cov_6.648936, whole genome shotgun sequence.
ACCESSION   DAOMQG010000455 DAOMQG010000000
VERSION     DAOMQG010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16746_length_2499_cov_6.648936"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..2252)
                     /locus_tag="PLC06_15895"
     CDS             complement(180..2252)
                     /locus_tag="PLC06_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRO91982.1"
                     /translation="MKEYQTGQIRNIALVSHNGAGKTMLVERILFKTGAITRMGSVAT
                     GTSHMDFEEEEIARNSTLVAAIAPVEWKNTKLNLLDTPGFLDFIGEVNSAMKVADSAA
                     VLVEAVSGVEVGTEAVWAAADAYGRPRVLVVNKMDRENVRVRRVKESINNNLSGHFVD
                     LQLPIGEGPAFKGVVDLLHMEARLGEKGDKAPIPADMQAAADEARAALVEAAAEGDDE
                     LIMKFLEGEELSGEEVARGLKEAILSGAVVPILYAAGETGIGVDALLDAFVELLPAPN
                     ETGGFEATNARGETATWEVSDKSPLTVFIFKSREDNFGKTSYMRVFGGALSSDSRIWD
                     PGRNAEVRVGALQITSGKDNSPVGKLHAGDIGTVVKLGDAATYDTLGDRGNVLIIAPV
                     EQPNPLASVAIHPVAQADTAKLSQSLNRLVAEDPTLHWSTEPSTHQTILSGMGVAHLD
                     IAVHKALSKFGVHLRTTTPKVPYRETITKSADADYTHKKQTGGAGQYARVMLRVEPVE
                     ESRGFEFGSEIFGGAVSAPFVAATEKGCRQALEGGVLAGYPVVGVRAVITDGKMHPVD
                     SKEIAFQTAGREGFKEAVRKAGPMLLEPIYEVAVTVPADNMGDVLGDMNSRRARVLGM
                     DQEGSKSIVRVEVPLAEMQTYAADLRSMTQGRGLFSMKFLQYGRVPAHLQDDLVAKLI
                     KEHEEGGE"
BASE COUNT          395 a          796 c          844 g          464 t
ORIGIN      
        1 gtcgacggcc gagagcgacc ggatcgcgct ggaagaatta ggaatcagaa atcaggaatg
       61 aagaggggcg agacaaccgg cgagccacgc gcaggtgatt cgatgaggcc acgcgccggt
      121 tgtcccgccc ctgcattaat tcccaattcg taattcgtaa ttcgtaattc gtaattcccc
      181 tactcccccc cttcctcatg ctccttgatg agcttcgcca ccaggtcatc ctgcaggtgg
      241 gcggggacgc ggccgtattg caggaacttc atgctgaaca gcccgcggcc ctgggtcatc
      301 gagcgcaggt cggcggcgta ggtctgcatc tcggccagcg gcacttcaac gcggacgatg
      361 ctcttgctgc cttcctggtc catgcccagc acgcgggcgc ggcggctgtt catgtcgccc
      421 agcacgtcgc ccatgttgtc ggccggcacg gtgacggcca cctcatagat cggctccagc
      481 agcatcgggc cggccttgcg caccgcctcc ttgaacccct cgcggccggc ggtctggaag
      541 gcgatttcct tcgagtcgac ggggtgcatc ttgccgtcgg tgatgacggc gcgcacgccc
      601 actaccgggt agccggccag cacgccgccc tccagcgcct gacggcagcc cttctccgtg
      661 gccgccacga acggggccga caccgcgccg ccgaagatct ccgagccgaa ctcgaagccg
      721 cgcgactcct ccaccggctc gacgcgcagc atgacgcgcg catattgccc cgcgccgccg
      781 gtctgcttct tgtgggtata gtcggcgtcg gccgatttgg tgatcgtctc gcggtaggga
      841 accttgggcg tcgtcgtgcg cagatggacg ccgaacttcg acagcgcctt gtggacggcg
      901 atatcgagat gggccacgcc catgccggag aggatcgtct ggtgggtcga cggctcggtc
      961 gaccagtgca gcgtcgggtc ttcggccacc agacggttga gcgactggct cagcttggcc
     1021 gtgtcggcct gcgccaccgg gtggatggcc accgaggcca gcgggttggg ctgctccacc
     1081 ggggcgatga tcagcacatt gccgcggtcg cccagcgtgt cataggtggc ggcgtcgccc
     1141 agcttgacca cggtgccgat gtcgccggcg tggagcttgc cgacggggga gttgtccttg
     1201 ccgctggtga tctgcagcgc gccgacgcgc acctcggcat tgcggccggg gtcccagatg
     1261 cgggaatcgg acgacagcgc gccgccgaag acgcgcatat agctggtctt gccgaaattg
     1321 tcctcgcggc tcttgaagat gaagacggtc agcggcgatt tgtcgctgac ttcccaggtg
     1381 gcggtctcgc ccctggcgtt ggtggcctcg aagccgccgg tctcgttggg ggcgggcagc
     1441 agttcgacga aggcatcgag cagggcgtcg acgccgatgc cggtctcgcc ggcggcatag
     1501 aggatgggca cgaccgcgcc gctgaggatg gcttccttca ggccgcgggc cacttcctcg
     1561 cccgacagct cctcgccctc gaggaacttc atgatcaact cgtcgtcgcc ttcggccgcg
     1621 gcttccacga gggccgcgcg ggcctcatcg gcggcggcct gcatgtcggc cgggatgggg
     1681 gccttgtccc ccttctcgcc gaggcgggct tccatgtgga gcaggtcgac gacccccttg
     1741 aaggccgggc cttcgccgat gggcagctgc agatcgacga agtggccgct gaggttgttg
     1801 ttgatgctct ccttcacgcg gcggacgcgc acgttctcgc ggtccatctt gttgacgacg
     1861 aggacgcgcg ggcggccata ggcgtcggcg gcggcccaga cagcctcggt gccgacctca
     1921 acgccggaga cggcctcgac cagcacggcg gcgctgtcgg ccaccttcat ggcgctgttg
     1981 acctcgccga tgaaatcgag aaagccgggg gtgtcgagca ggttcaactt ggtgttcttc
     2041 cactcgacgg gggcgatggc ggcgacgagg gtgctgttgc gcgcgatctc ttcttcttcg
     2101 aaatccatgt gggatgtgcc ggtggcgacg ctgcccatac gcgtgatcgc cccggtcttg
     2161 aagagaatgc gctcgaccag catcgtcttg ccggcgccgt tatgggaaac aagggcgata
     2221 ttgcgaattt gtccggtctg atattccttc atgagtcagt tcaacctcta tggtaatttg
     2281 gtaactatgg aaccgggccg ggtggccagg ctcctcgcgc ggggcgatgg gtatccgtga
     2341 tgggcggcgc ggcggggtaa acgcaaacag gacggatcgc aacgcgcggg gcgtcacaag
     2401 ccgtcctgtt cggttcaatc cgtgtccggt tggtccatca caaaaagaaa cccggttccc
     2461 cggtcgaaat ttaaaatgat acgatggcgg agcggccgc
//
LOCUS       DAOMQG010000456         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16932_length_2475_cov_12.727273, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000456 DAOMQG010000000
VERSION     DAOMQG010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16932_length_2475_cov_12.727273"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1007
                     /locus_tag="PLC06_15900"
     CDS             360..1007
                     /locus_tag="PLC06_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO91983.1"
                     /translation="MLRLLIVDDHEVVRLGMRALLERHPTFTVVGEASTQEEAVAMAE
                     SLQPDIVLMDIRLAGGSGIEACQQIKDRLPQIKVIMLTSFAEDELLFAAIRAGATGYL
                     LKQIAGGDVIRAIEGAARGESMLDPSLTQRVFLEMRRSIKKEEAVAFQDLTSQERQVL
                     LLIADGRTNREIATELFLSEGTVRNYVSSILSKLGVSNRAEAAAYAIKHHLADHT"
     gene            complement(1100..1654)
                     /locus_tag="PLC06_15905"
     CDS             complement(1100..1654)
                     /locus_tag="PLC06_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91984.1"
                     /translation="MNNSIVKIHANPGPFLKNGKAFNLGIEPSVLDGHGRMSRQHLDQ
                     ALIGIAEFIITDLVGQIEVAQRFPRGDNRHAKKIIHKRVTLGHTDGSRVSRDMRHPDR
                     LLTMDHLSQKPFPQRPMSDFFDIFSLHPTDDKTMNVTRAGQTPISHGQRRILCAYQVA
                     SRIDDQLQDLIYVKLTGYGESSLV"
     gene            1667..2203
                     /locus_tag="PLC06_15910"
     CDS             1667..2203
                     /locus_tag="PLC06_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO91985.1"
                     /translation="MGLTLESTRLALPSGAEEVSALLDTAIEGLNDAIRDIRNFILDL
                     RPRRFAGDVQQGLAQLVREFQANTMVPVSISVPERLEDLPLPQGRAIFLTTQEALANI
                     ARHARATKVNITLHYSTEHVVLAIHDNGRGFDAGNESLRIGHGLANMQARAESLRGVF
                     TIHSLPGQGTDVTLDLPL"
BASE COUNT          518 a          613 c          730 g          614 t
ORIGIN      
        1 gagaagatgg ccggtatatt gccgccggag gcagaggggg gtattctgta tgggtgatga
       61 cggaatcgcc aaagctcccc aggcccggct catccgatag aaaatggtgg aaacaggagg
      121 cggccaacag gccaaaatcc tgtgaagcgc cgccacgcga gggcaaccca tgcccatctt
      181 gtttgcttgg tgagctttat tacaacgggc tatttcagct gatttgcgac aattgcggcc
      241 gggttgccga ggcagccgtc tttacctgat aggcttgcgt gggcggctgt aggcttgcct
      301 catccataaa cattgtaagc aatgtgttct tttagctata cttggtctgt atgatccaga
      361 tgttgcgctt gctgattgtc gatgatcacg aagtagtccg ccttgggatg cgtgccttgc
      421 ttgaacgcca ccctacgttc acagtcgtcg gtgaagcctc gactcaggaa gaggcggtgg
      481 cgatggccga gtccctgcaa cctgatatcg tgttaatgga tattcgactg gccggcggta
      541 gtggcatcga ggcttgtcag cagatcaagg atcggcttcc ccagattaaa gttatcatgc
      601 ttacctcgtt cgccgaggat gaattgctgt ttgcagccat tcgcgccggg gcgacgggtt
      661 atctgctgaa acagatcgcc ggtggcgatg tcattcgggc gatcgagggc gctgcgcgag
      721 gcgaatccat gctggatcct tcattgaccc agcgtgtttt tctcgagatg cgccggtcta
      781 tcaagaaaga agaagctgtt gcttttcagg acttgacgag ccaggagcga caagtgttgt
      841 tgctgatcgc cgatgggcga acgaatcgcg agattgctac tgagttgttc ctgagcgagg
      901 gaaccgtccg aaattatgtg agcagtattc tttcaaaatt gggcgtttcc aatcgtgccg
      961 aggcggccgc gtatgcaatc aagcatcatc tggcagatca tacttgattc gtttgttatc
     1021 caggcgtttg tatctcgctg tggaaagccc caacataccc caaatgaatg atctgtccgc
     1081 ccaacgtgcc ttgggagcgt taaaccaggc tgctctcgcc atagccagtg agcttgacgt
     1141 agataaggtc ctgcaattga tcgtcgattc ggctcgcgac ctggtaggcg cagagtatgc
     1201 ggcgctggcc gtgggagatt ggcgtttgcc cggcgcgggt aacgttcatc gttttgtcgt
     1261 cagtgggatg gagcgagaag atatcaaaaa aatcgctcat tggccgctgg ggaaagggct
     1321 tttgggagag gtgatccatg gtcaacaacc tgtccgggtg acgcatatcg cggctgacac
     1381 gcgatccgtc ggtatgcccg agggtcaccc tcttatggat gattttcttg gcgtgccgat
     1441 tgtcgccgcg ggggaaacgc tgggcaacct ctatctgacc gacaagatcg gtgatgatga
     1501 attcagcgat gccgatcaac gcctgatcga gatgctggcg gctcatgcgg ccgtggccat
     1561 ccagaacgct cggctctatt cccaggttga acgccttgcc attcttgagg aaaggacccg
     1621 gattggcatg gatcttcacg atggagttat tcaatcgatc tatgcagtgg gcctcacgct
     1681 ggagtctacg cgactggcgt tgccgagtgg cgcagaagaa gtttccgctt tgttggatac
     1741 ggctattgaa gggctgaacg atgcaattcg cgacattcgt aactttatcc tggacttgag
     1801 gccgcggcgg tttgccgggg atgttcagca ggggttggct caactcgttc gcgaatttca
     1861 ggccaacacg atggtccccg tctccatttc tgtccctgag cgtctggaag acctgccgct
     1921 gcctcaaggt cgagccatct ttctgacgac ccaggaagct ctggcgaata tcgcgcgcca
     1981 cgcgcgagca acgaaggtaa acattacgct tcactattcc acagagcacg ttgtgttagc
     2041 catccatgat aacggccgcg gctttgatgc tggcaatgaa tcattgcgga ttgggcatgg
     2101 cctggccaac atgcaagccc gagccgaaag cctgcgtggt gtatttacaa tccactcctt
     2161 gcctggccaa gggactgacg taactctcga tttgcccctc taagtgggtg agccattcct
     2221 ctgattttcg gcaactctgg tcgattcaat ctcataacag tgggtgatag atgtcatccc
     2281 tatcgatgac ttttgtcatt gagtttgcgc gccggcaagg gatatactgc ctctaatgac
     2341 aagtgtcgcg gttgcccgtc cttagggtgc agttgaatca tttgcagttg ttaactgtgg
     2401 ctgatgaagg ccacgcgtag tttgaggggg ctggtgatgc ttcagtcata cttcgaaatg
     2461 gatgaaatgt tgagc
//
LOCUS       DAOMQG010000457         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16933_length_2475_cov_7.764050, whole genome shotgun sequence.
ACCESSION   DAOMQG010000457 DAOMQG010000000
VERSION     DAOMQG010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16933_length_2475_cov_7.764050"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLC06_15915"
     CDS             <1..363
                     /locus_tag="PLC06_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91986.1"
                     /translation="LLADGELRPLTEDHSLVRRLQEAGRLSEEDAQHFEYRNVLLQAI
                     GQDNPLTADTFSLDLPDAGKLLLCSDGLCGLVSLEEIAVIMLGPDKPQAIADRLYQAA
                     MAAGGHDNITAIVVDFSF"
     gene            403..1884
                     /locus_tag="PLC06_15920"
     CDS             403..1884
                     /locus_tag="PLC06_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRO91987.1"
                     /translation="MEQELSNLYAVLGVGRRATPDEITAAFDAWNARADAGEPIDDPL
                     WERIRYAWDVLSSPSRRDLYDSLVDDATGGDALGLDLLVSAETLPLLDTPQLVYALVT
                     LRPGETTGERRPLNLSLVIDRSTSMRGERLERVMAAVGLLIKKLGPDDTLSVISFSDR
                     AEVVLPAAALGRAAQSATPEIAAAWREPDRRLRGIVASGGTEIFYGLRAGLDQIARGG
                     MDGHTNHLILLTDGHTYGDADECLRLVHDAAARGIGLTAFGLGTDWNDQFLDALVAPS
                     GGQSRFIESADEVIRHLEGRLSGLGDIYARNLSLPHAWPGQLTLRGGFKLTPFPQPLA
                     IGSDPTPLGDLEGRAPVTMLLEFLVAPQSMASRFRLPVEIRYTATGGKEERIERFAQI
                     IIHESAAAEAAVPAPLIEAVLRLNLYRMQEKAWEDAQSGKLDTAAARMRRLTARYMET
                     GDLRLARQAQLEAQQLAHLGTMSLEGRKLLKYGTRSLMGQLDL"
     gene            1881..2453
                     /locus_tag="PLC06_15925"
     CDS             1881..2453
                     /locus_tag="PLC06_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRO91988.1"
                     /translation="MKHCPDCGAEHYPGALFCDMCGAAVHAAARAHVTAGRQAVARPP
                     VIQPATPSRRVTESGRFESVILPAAPAPAMPPRALHIRLPHHDATLILTGALIQVGRA
                     DPGSVHADPGSVHAPELDLTTYGGQERGVSRRHATIQWVEGSYVLIDQHSSNGTWLND
                     VRLVPGYAYQIPPGAKVRFGGLLVQLTIAD"
BASE COUNT          415 a          909 c          771 g          380 t
ORIGIN      
        1 ctgctggccg acggcgaact gcgcccgctg accgaagacc attccctggt gcgccgcctg
       61 caggaagcgg gacgcctttc cgaagaagac gcccagcatt tcgaatatcg caacgtgctg
      121 ctccaggcaa tcggccagga caacccgctg acggccgaca ctttctcgct cgatctgccc
      181 gacgccggca agctgctgct gtgcagcgac ggcctgtgtg gcctcgtcag cctggaagaa
      241 atcgccgtca tcatgctcgg ccccgacaag ccccaggcga tcgccgaccg gctctatcag
      301 gcggctatgg ccgccggcgg ccacgacaac atcaccgcca tcgtggttga tttttctttc
      361 taatggtcga ttctcgcggg cgaagaaccg gccgattggc ttatggaaca ggaactctct
      421 aacctgtacg ccgtcctggg cgtcggtcgc cgggccacac cggacgagat cacggcggcc
      481 ttcgacgcct ggaacgcccg ggccgacgcc ggggagccga tcgacgatcc attgtgggag
      541 cgcattcgct atgcctggga cgtcctcagc agcccctcgc gccgcgacct ctacgattcg
      601 ctggtcgacg acgcgaccgg cggcgacgcc ctgggcctcg acctgctcgt cagcgccgag
      661 acgctgccgc tgctcgacac cccgcagctc gtctacgccc tggtcacctt gcgccccggc
      721 gaaacgaccg gcgagcgccg cccgctgaac ctgagcctgg tcatcgaccg cagcacctcg
      781 atgcgcggcg aacggctgga gcgagtcatg gccgccgtcg gcctgctgat caaaaaactg
      841 gggccggacg acacgctgtc ggtcatcagc ttcagcgatc gggccgaagt cgtgctgccc
      901 gcggccgcgc tgggtcgggc cgcccaaagc gccactcccg aaatcgccgc cgcctggcgc
      961 gagcctgacc gccgcctgcg tggcatcgtc gcctccggcg gcaccgagat attctacggc
     1021 ctgcgcgccg ggctggacca gatcgctcgc ggcggcatgg atggccacac caaccatctg
     1081 atcctgctga ccgacggcca cacctatggc gacgccgacg aatgtctgcg gctggttcat
     1141 gacgccgccg cccgcggcat cggcctgacc gctttcgggc tggggacgga ctggaacgac
     1201 cagtttctcg acgcgctggt cgccccctcg ggcggccaat cccgtttcat cgagagcgcg
     1261 gacgaagtga tccgccatct ggaagggcgg ctcagcgggc tgggggacat ctacgcccga
     1321 aacctgagct tgccccacgc ctggcccggc cagctaaccc tgcgcggcgg cttcaagctg
     1381 accccctttc cccaaccgct ggccatcggc tccgacccca ccccgctggg cgatctggaa
     1441 gggcgggccc ccgtgacgat gctgctggaa ttcctcgtcg cgccccagtc gatggcgtct
     1501 cgcttccggc tgcctgtcga gatccgttac acggcgacgg gcgggaaaga ggaacggatc
     1561 gaacgattcg cccaaatcat catccacgag agcgccgcgg ccgaagcggc cgttcccgcg
     1621 ccgctcatcg aggccgtcct gcgcctgaac ctctatcgca tgcaggaaaa ggcgtgggag
     1681 gacgcccaat cgggcaagct ggacacggcc gcggcccgaa tgcgccgcct gaccgcccgc
     1741 tacatggaga ccggcgacct gcggctggcg cgccaggccc aactggaggc ccagcagctg
     1801 gcccatctgg gcacgatgtc gctcgaaggc cgcaaactgc tcaaatatgg cacgcgctcg
     1861 ctgatggggc agctggatct atgaaacatt gtcccgattg tggtgcggag cattaccccg
     1921 gcgcgctctt ttgcgatatg tgcggcgcgg ccgtccatgc cgcggcgcga gcgcacgtca
     1981 ccgccgggcg gcaggcggtc gctcgcccgc ccgtcatcca accggcaacc cccagccgtc
     2041 gcgtcaccga gagcggccgc ttcgaaagcg tcattctccc cgccgcgccg gccccggcca
     2101 tgccgccccg agcgctccac atccgccttc cccatcatga cgcaacgttg atattgaccg
     2161 gcgcactcat ccaggtgggc cgcgccgacc ccggcagcgt ccacgctgac cccggcagcg
     2221 tccacgcgcc ggaactggac ctgacgacct atggcggcca ggagcgcggc gtctcgcgcc
     2281 gccacgccac catccagtgg gtcgaaggca gctacgtgct catcgaccag cacagcagca
     2341 acggcacctg gctcaacgac gtgcgcctcg tccccggcta cgcttatcag attccccccg
     2401 gtgcgaaggt cagattcgga ggattactgg tacaactgac gatcgcggat taaacttggg
     2461 acaggtgcgc catgc
//
LOCUS       DAOMQG010000458         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16978_length_2470_cov_9.409938, whole genome shotgun sequence.
ACCESSION   DAOMQG010000458 DAOMQG010000000
VERSION     DAOMQG010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16978_length_2470_cov_9.409938"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2470)
                     /locus_tag="PLC06_15930"
     CDS             complement(<1..>2470)
                     /locus_tag="PLC06_15930"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRO91989.1"
                     /translation="SPIRTTLFVIILFLTLTGLTGVGALTAQADPAPAASPTPTEDQR
                     NDASIPPLVGGSPATPKTAVPAPRGPANPGTRFQDSPNAPAITLWYGPTQTFGPNGDP
                     QKWVNIVGNVAGAVSLTYSLNGSPAKTLAIGPNNSRLGSSGDFNIELNYIDLNAGNNT
                     VTITATDSLSVTTQAIVTVNYQARPGAWTPGTYTIDWSTASKVTDVAQVVDGNWVINA
                     GKAKTAAAGFDRLIAIGDMSWRDYTVTVPVTVHSMDQNKSPGVGFIVRWWGHFDNGAG
                     LQPLVGWKRLGAMAWYRYEKATATEGLQLLGDGGATLGTKGFSLTLGTTYMYKVSVTS
                     NADPKKPATYRFKVWPQAQTEPAAWDFELPGVVGEPPSGSVVLLAHHADVSFGNATVM
                     LNSVASKPKLTLGTMGTGTGSVAANPQKATYRFGEDVSLMATANAGSTFVDWQGDAKG
                     TLNPTAIEMFADRNAKAHFTDPTVQTPLSDDFNGCVLNPSLWTFVDPLGDSTVTMSGG
                     HAEISVPAGTTHDLWINGRNAPRIMQFADNNNFEFSVKFDSSMDKKNQAQGILVEGDA
                     QNYLRFNYLHDGSSYRIQAYTFTGGVETNRITSDNKITIGPPMYLRVKRIGDFWNLFY
                     SANGSTWSLAAGFSYSLGVTSIGVYAGNSNVNPAHTAKIDYFFNSNSPISPEDNARKL
                     NVTIDGSGSVQRTPDKENYACDEVVTLTPVPAPGYKFDSWSGALTGNANPAQLTMNAT
                     KDVVARFVSDTQYTVTLSANGAGTVTKSPEKATYGAGETVTLEATPNLGNTFVNWLVN
                     GVPNTANPLVVTVNSNMTVVGNFAP"
BASE COUNT          463 a          697 c          780 g          530 t
ORIGIN      
        1 ggggcaaagt tgccgacgac cgtcatattg ctgtttactg tgaccaccag cgggttggcc
       61 gtattaggca ccccattgac cagccagttg acaaaggtat tacccaggtt cggggtggcc
      121 tccagcgtca ccgtttctcc ggccccatag gtcgccttct cgggagactt ggtcaccgtg
      181 cccgcgccgt tggcgctgag cgtcacggta tattgcgtgt cgctgacgaa cctggcgacg
      241 acgtccttgg tggcattcat ggtcagttgg gccgggttgg cattgccggt tagcgcgccg
      301 ctccacgaat cgaacttgta gcccggcgcg ggaaccggcg tcaatgtcac cacctcatcg
      361 caggcatagt tctccttgtc gggagtccgc tgcacagagc cgctgccgtc gatagtgacg
      421 ttgagcttgc gggcgttgtc ttcgggcgag atcgggctat tgctgttgaa gaagtaatcg
      481 atcttggcgg tgtgggccgg gttcacgttg gagttgccgg cataaacacc aattgatgtg
      541 acgccaagcg agtacgagaa tccggcggcc aggctccagg tagatccatt ggcagaatag
      601 aaaagattcc agaaatcgcc gatacgcttg acacgcaggt acatcggggg ccctatcgtg
      661 attttgttgt cactggtgat gcgattcgtc tccacaccac cggtaaacgt gtaggcttga
      721 atcctgtagc tgctaccgtc gtggaggtag ttgaagcgca gatagttttg cgcgtcaccc
      781 tccacaagga tgccctgggc ctgattcttc ttgtccatcg acgaatcaaa cttaacggag
      841 aactcaaagt tgttgttatc ggcaaactgc atgatgcgcg gcgcgttgcg gccgttaatc
      901 cacaggtcgt gggtcgtgcc cgccggcacg ctgatctcgg catgaccgcc gctcatcgtc
      961 acggtgctgt cgcccagtgg gtcgacaaac gtccatagcg acggattgag cacgcagcca
     1021 ttaaaatcgt cggagagagg tgtctgcaca gtgggatcgg tgaagtgggc tttcgcgttc
     1081 cggtcggcga acatctcgat ggccgtcggg ttcaacgtgc ccttggcatc accttgccag
     1141 tcaacgaagg ttgaaccggc gttggccgtc gccatcaggc taacgtcttc gccgaagcga
     1201 taggtggcct tctgtgggtt ggcggcaacg ctgccggtgc cggtacccat cgtgccgagc
     1261 gtgagcttcg gcttggacgc gacgctgttc agcataacgg tcgcattgcc gaagctgacg
     1321 tcggcatggt gcgccaataa tacgacagac ccactgggcg gttcaccgac gacgccgggc
     1381 agctcaaaat cccacgcggc cggctcggtc tgtgcctggg gccagacctt gaaccgataa
     1441 gttgcgggtt tcttcgggtc ggcattcgag gtgacgctga ccttgtacat atacgtcgta
     1501 cccagcgtca gactaaagcc tttggtgcct agggttgcac caccgtcgcc caggagttgc
     1561 aggccttcgg tggctgtcgc cttttcatag cgataccagg ccatcgcgcc cagcctcttc
     1621 cagccgacaa ggggttgcag ccctgcgccg ttgtcgaagt gaccccacca gcgcacaatg
     1681 aagcccacac cggggctttt attctggtcc atcgaatgca ccgtaaccgg cacggtgacg
     1741 gtgtaatcgc gccaggacat atcgccaatc gcgatgagtc gatcgaagcc cgccgcggca
     1801 gtcttcgcct tgccggcatt gatcacccaa ttaccatcaa cgacctgggc tacgtcagta
     1861 accttgctcg ccgtgctcca gtcgattgtg taggtgccgg gagtccatgc gccaggccgg
     1921 gcctggtaat tgacggtgac aatagcctgc gtcgtaacgc tgaggctatc ggtggcagtg
     1981 atagtgacgg tgttattgcc ggcgttcagg tcaatgtagt taagctcgat attgaaatcg
     2041 ccgctgctgc ccagacggct gttgttgggg ccgatggcca gcgtcttcgc cgggctgccg
     2101 ttgagcgaat aggtcaagct cacagcgccg gccacattgc cgacgatgtt gacccacttt
     2161 tgtggatcgc cattggggcc aaaagtttgg gtggggccat accagagagt gatggccggc
     2221 gcattgggcg aatcctggaa gcgcgtgcca ggattggccg ggccgcgcgg cgccggaacg
     2281 gccgtcttgg gcgtagccgg gcttccccca accagaggcg ggatggatgc atcgttgcgc
     2341 tggtcttccg tcggcgttgg cgaagccgcg ggagcggggt cggcctgggc agtcagagcg
     2401 cccacaccgg tgagaccggt cagggtcagg aacaggatga ttacgaataa agtagttctg
     2461 ataggagatg
//
LOCUS       DAOMQG010000459         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_16996_length_2468_cov_6.252383, whole genome shotgun sequence.
ACCESSION   DAOMQG010000459 DAOMQG010000000
VERSION     DAOMQG010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16996_length_2468_cov_6.252383"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..468
                     /locus_tag="PLC06_15935"
     CDS             1..468
                     /locus_tag="PLC06_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HRO91990.1"
                     /translation="MKLSEILEDYGGWLAFIVAATAMAGSLYYSEVAGFIPCRLCWYQ
                     RILMYPLVAVTFVGALRRDDYLPGYVLPLSLLGMGVSTWHYMMEKGFVPASTTCAADV
                     PCNITYVNYLGFITIAFMALVAFTLITLIMFGMWNAYRRESAGRLPIPDEGAY"
     gene            469..864
                     /locus_tag="PLC06_15940"
     CDS             469..864
                     /locus_tag="PLC06_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRO91991.1"
                     /translation="MRLFAVAGVLMALLLLVAGCAIGPDAAPTPTPTPVDPAALGRQT
                     WTSYCVPCHGANGEGFINALNAPALNAGGESYRLTDAAILSAIIDGGAASGGAMAPLG
                     DALSDAQEAAVLEYVHTLWTDEQRAAHVH"
     gene            1006..>2468
                     /gene="hemW"
                     /locus_tag="PLC06_15945"
     CDS             1006..>2468
                     /gene="hemW"
                     /locus_tag="PLC06_15945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRO91992.1"
                     /translation="MYALYLHIPFCRARCSYCDFNTYTTLGDLQGEYVAALAREIRLV
                     GDTGRAGEAGRAGDERAAVDTIFFGGGTPSLLTPEQVATLLAAVRDSFALASDAEITM
                     EANPGTVDEAWLAAVRALGVNRLSFGVQSALPHELALLGREHDFAAAVTALSAARAAG
                     FDNLNLDLIYGLPGQSVADWQRTLDAALPLGVEHVSLYCLTIEPGTPMQRWLHNGTIM
                     APDPDTAADQYEAACRALAAAGYGHYEISNWAKPGRECRHNIVYWRNRPYLGLGAGAH
                     GMAGGYRYQVVRQPRTYIKRITNYELRITNGEPRATNDEPRAANDERRGGHRPPATDL
                     LTTDLLNTDLLTTDLLNTDPLTTDLLNTDPLNTDLLITDPLTTDPLTTGHSPPATLPV
                     SPSPLPPFPLSPAVAAHHRVDEAEAMSDTAITQLRLLDEGLDLGAFEARFGRAFDEVY
                     RDEVRQLVDWGLLRVDGHRLLLTEKGRFLSNQVFYRFV"
BASE COUNT          410 a          829 c          779 g          450 t
ORIGIN      
        1 atgaaattat ctgaaatcct cgaagattac ggcggctggc tggcgttcat cgtcgccgcg
       61 accgccatgg cgggcagcct ctactacagc gaagtggccg gcttcatccc ttgccgcctg
      121 tgctggtatc agcgcatcct catgtatccg ctcgtcgccg tcaccttcgt cggcgcgctg
      181 cggcgggatg actacctgcc cggctatgtg ctgccgctgt cgctgctggg catgggtgtg
      241 tcaacctggc attatatgat ggaaaagggg ttcgtccccg cgtccaccac ctgcgcggcc
      301 gatgtgccct gcaatatcac ttatgtcaac tatctgggct tcatcaccat cgccttcatg
      361 gccctggtcg ccttcacgct catcacgctg atcatgttcg gcatgtggaa cgcttaccgg
      421 cgcgagagcg cgggccggct gcctatcccc gacgagggag cgtactagat gcggcttttc
      481 gcggttgcgg gcgtgctgat ggccctgctc ctgttggtgg ccggttgcgc catcgggccg
      541 gacgccgccc ccacgcccac gccgaccccg gtcgatccgg cggcgctggg gcggcagacg
      601 tggacgtctt actgcgtgcc ctgccacggc gcgaacggcg aagggttcat caatgcactc
      661 aatgctccgg ccctcaacgc cggcggcgag agctaccggc tgaccgacgc ggcgatcctc
      721 tcggccatca tcgacggcgg cgcggcgagc ggcggcgcga tggccccgct gggcgacgca
      781 ctgagcgacg cgcaggaagc ggccgtgctg gaatatgtcc acacgctctg gaccgacgag
      841 cagcgcgccg cgcatgtgca ttgagggaag tgggccagtg ggtcagtggg tcagtgggtc
      901 agtgggtcag tgggtcagtg ggtcagtggg tcagtaggtc agtagatcag tagatcagcc
      961 cggtcttcat tcgtaattcg taattcgtaa ttcgtaattc tccccatgta cgccctctat
     1021 ctccacatcc ccttctgccg cgcccggtgc agctattgcg acttcaacac ctacaccacc
     1081 ctcggcgact tgcaggggga atatgtcgct gccctggcgc gggagattcg gctggtgggc
     1141 gacacggggc gtgcggggga agcgggtcgc gcgggcgacg aacgcgcggc cgtcgacacg
     1201 atcttctttg gcggcggcac gccgtcgctg ctgacgcccg aacaggtggc gacgctgctg
     1261 gcggccgtgc gcgattcgtt cgccctggcc tccgacgcgg agatcaccat ggaagccaac
     1321 cccggcacgg tggacgaggc ctggctggcg gccgtccggg cgctgggggt caaccggctc
     1381 agcttcggcg tgcagagcgc gctgccgcat gagctggccc tgctcggccg cgagcatgat
     1441 ttcgccgcgg ccgtgaccgc cctgtccgcc gcccgcgccg ccggcttcga caacctcaat
     1501 ctcgacctca tctacggctt gccggggcag agcgtcgccg actggcagcg cacgctggac
     1561 gccgcgctgc cgctgggcgt ggagcacgtg agcctctact gcctgaccat cgagccgggc
     1621 acgccgatgc agcgctggct ccacaacggc acgatcatgg cccccgaccc cgacacggcc
     1681 gccgaccagt acgaggccgc ctgccgcgcg ctggccgccg ccggctatgg gcattacgag
     1741 atatccaact gggcgaagcc ggggcgcgaa tgtcgccaca acatcgtcta ttggcgcaac
     1801 cggccctatc tgggcctcgg cgcgggcgcc cacgggatgg ccggcggcta ccgctatcaa
     1861 gtcgtgcgcc aaccgcgaac atacatcaag agaattacga attacgaatt acgaattacg
     1921 aatggggaac cacgagcgac gaacgacgaa ccacgagctg caaacgacga acggcgaggc
     1981 ggccaccggc caccggccac tgatctactg accactgatc tactgaatac tgacctactg
     2041 accactgatc tactgaatac tgacccactg accactgatc tactgaatac tgacccactg
     2101 aatactgacc tactgatcac tgacccactg actactgacc cactgaccac cggccactct
     2161 ccgccggcca ctctccccgt ctccccttcc ccccttcccc ccttccccct ctcccccgcg
     2221 gtggcggcgc atcatcgggt ggatgaggcc gaggcgatgt cggacacggc cattacgcag
     2281 ctgcggctgc tcgatgaggg gctggatttg ggcgcgttcg aggctcgctt cggccgcgct
     2341 ttcgatgagg tctaccgcga cgaagtgcgg cagctggtcg attggggcct gctgcgcgtg
     2401 gacggccacc gcctgctgct gacggaaaag ggacgatttt tgagcaatca ggtgttttat
     2461 cggtttgt
//
LOCUS       DAOMQG010000460         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17012_length_2466_cov_9.729158, whole genome shotgun sequence.
ACCESSION   DAOMQG010000460 DAOMQG010000000
VERSION     DAOMQG010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17012_length_2466_cov_9.729158"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1198
                     /locus_tag="PLC06_15950"
     CDS             176..1198
                     /locus_tag="PLC06_15950"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRO91993.1"
                     /translation="MFKPRYSITPELLRDIKDITVLVHELNKQVVPEVVLMHRQTEAR
                     SISTYASTSIEGNPLPLTDVKRLLKSQPAQLRHSEREVVNYNDALIALSEQRDSMLDT
                     ALLLRIHAGVMDGLLPKHQVGRFRLEPVVISEPRTGDIIFLPPDHQDVPALIEELLTF
                     VRENRSLDPLLMAGLFHKQFVIIHPFMDGNGRTTRLATDHLLNGLGLRLMGLFSFENY
                     YNQNVSRYFQMVGAAGNYYDLADGPDFTPWLEYFAAGILDELQRVGKELAGRRTPETN
                     LNPHDRLILAHIDAHGYINDKEYARLTERAKATRTLDFNRLIRLGLIKRQGSGRNTHY
                     RRTDEP"
     gene            complement(1143..>2466)
                     /locus_tag="PLC06_15955"
     CDS             complement(1143..>2466)
                     /locus_tag="PLC06_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRO91994.1"
                     /translation="MNRITSPQDIDRLEDLIGRREPELLALLPEEGRFDRLRRELDAL
                     NRRYPDPATRPPLFGALVGVKDIFNIDGFPTRAGSDLPPELFAGPEAACVTALKSAGA
                     LILGKTVTTQFAYFAPGPTRNPLSAALGEIRTPGGSSSGSAAAVAAGFCSLALGTQTI
                     GSIIRPASFCGVVGYKPSFGRVPSDGALPLSPSADTVGWFGGTMEDVALMADVLIPDW
                     TAGGRPPTAVRGESAVGRRPSAVTLGIPDGPYLERASAEGLAHFWQTVRRLEAAGVEI
                     RRVPAMADYDDIYRRHNDIVAYEAARTHARWFDAYREQHHPKTAELIERGRTISEAGY
                     RRALDGRARLRDELHGLMDAHGLDLWIAPAAVGAALPGLESTGDPVMALPWTHAGLPA
                     VSLPAGTDAAGWPMGLQVVGRFGRDEELLALTARLSVDNGYFGRSPAV"
BASE COUNT          466 a          788 c          774 g          438 t
ORIGIN      
        1 cctggggttc tgtttgcatc ttgttattta gaacttgcgc agaagtacag ggaacggtat
       61 agccattggt gggcaaaccc aacccggatc gtaccggcct ttattatccc aacttcccgt
      121 tatttttgcg tatgttccag ttagtcgata aataggctat aattaggcga aagaaatgtt
      181 taagccacgc tattccatta cgcccgaact tctgcgcgac atcaaggaca tcacggttct
      241 ggtgcatgaa ttgaacaagc aggttgtgcc cgaggtcgtt cttatgcacc ggcaaactga
      301 ggcgcggagc atctcgacct acgcttcaac gagcatcgag gggaacccgc tgcccctaac
      361 ggatgtcaag cggctgctca aaagccaacc cgcgcaactc cgccacagcg agcgggaggt
      421 ggtcaactat aacgatgccc tgatcgcgtt gagcgagcag agagactcga tgctggacac
      481 cgcgctgctg ctgcgcatcc acgccggagt gatggatggg ttgttgccga agcatcaggt
      541 cgggcgattt cgcctggagc cggtcgtcat cagcgaaccg cgcaccggtg acatcatctt
      601 tctgccgccg gatcatcagg atgtccccgc actgattgag gagctattga ctttcgtaag
      661 agaaaaccgg agccttgatc cgctgctgat ggcgggactc tttcacaaac aattcgtcat
      721 catccatccc ttcatggacg gcaacggccg cacgacacgg ctggcgaccg atcatctgtt
      781 gaacggtctg ggccttcgcc tgatggggct gttcagcttc gagaattact acaaccaaaa
      841 cgtcagtcgc tacttccaaa tggttggggc cgccggcaac tactacgatc tggcggatgg
      901 gccggatttc accccctggc tggagtattt cgccgcgggc atactggatg agttgcagcg
      961 ggtcggcaag gagttggccg gtcggagaac cccggaaacg aatctgaacc cccacgaccg
     1021 gctgatcctt gcccacattg acgcccacgg ctacatcaac gacaaagagt atgcccggct
     1081 gacggagcgg gcgaaagcca cgcgcaccct cgatttcaac cgcctcattc gcctcggctt
     1141 gatcaaacgg caggggagcg gccgaaatac ccattatcga cggacagacg agccgtaagc
     1201 gccagcaatt cttcgtccct gccgaatcgc cccaccacct gcaaccccat cggccagccc
     1261 gcggcgtcgg ttccggcggg caatgagacg gccggcagcc cggcgtgggt ccacggcaaa
     1321 gccatgaccg gatcgccggt gctttccagc cccggcaggg cggcccccac cgccgccgga
     1381 gcgatccaca gatcgaggcc gtgggcatcc atcagtccat gcagctcgtc gcgcagccgc
     1441 gcccggccgt cgagcgcgcg ccggtagccg gcctcgctga tggttcggcc gcgttcgatg
     1501 agttcggccg ttttggggtg gtgctgctcg cgataggcgt cgaaccagcg cgcatgcgtc
     1561 cgcgccgcct cataggccac gatgtcatta tgccgccggt agatgtcgtc gtagtcggcc
     1621 atcgccggca cgcgccgtat ctcgacgccc gccgcttcca atcgacgcac ggtctgccaa
     1681 aaatgggcca atccctcggc cgaggcgcgt tccaggtagg ggccgtcggg gatgccgagg
     1741 gtgaccgccg acggccgacg gccgaccgcc gattcccctc ttactgcggt cggcggtcgg
     1801 ccgccggcgg tccaatcagg aattaacacg tctgccataa gcgccacatc ctccattgtc
     1861 cccccaaacc agccaaccgt gtcggccgag ggggacaggg gcagcgcgcc gtcggacggg
     1921 acgcggccaa agctgggctt gtagccgacg acgccgcaga acgacgccgg gcgaatgatg
     1981 gagccgatgg tctgcgtgcc cagggccagc gaacagaacc cggcggccac ggccgccgcc
     2041 gagccgctgc tgctgccgcc gggcgtgcgg atttcgccca gtgccgcgct cagcgggttg
     2101 cgcgtgggac cgggggcgaa gtaggcgaat tgcgtggtca ctgttttgcc caggatgagc
     2161 gccccggccg acttcagcgc cgtcacgcag gccgcttccg gtccggcgaa taactccggc
     2221 ggcaggtcgc tccccgcccg cgtggggaag ccgtcgatgt tgaagatgtc cttgacgccg
     2281 acgagcgcgc cgaagagggg cggccgcgtc gccgggtcgg gatagcggcg gttcagcgcg
     2341 tccaattcgc gccgcaggcg gtcaaagcgg ccttcttcgg gcaacagggc cagcagttcc
     2401 ggctcgcggc ggccgatgag gtcttccaac cggtcgatat cctggggaga ggtgatacgg
     2461 ttcatg
//
LOCUS       DAOMQG010000461         2461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17050_length_2461_cov_7.503325, whole genome shotgun sequence.
ACCESSION   DAOMQG010000461 DAOMQG010000000
VERSION     DAOMQG010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17050_length_2461_cov_7.503325"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..690)
                     /locus_tag="PLC06_15960"
     CDS             complement(256..690)
                     /locus_tag="PLC06_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRO91995.1"
                     /translation="MRALLDTNVFISYLLTPQHAGSVKVVFEALGRNQFTLLLPDEVV
                     DEILDVIANRPHLMGRVGSDKLTTFLKLLTTVAEGILRIEQAIPAIARDAKDDYLLTY
                     AVVGQADYLVTGDKDLLVLEEIARVKIVSTVAFADLLSATTP"
     gene            complement(687..1028)
                     /locus_tag="PLC06_15965"
     CDS             complement(687..1028)
                     /locus_tag="PLC06_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRO91996.1"
                     /translation="MQKTVSATEAKAKLSDLMKWAVKTGDSVVVQSRGYPQVVIVPFA
                     EYQELRQAKENIRRTQAITQLQEIAREMQKASEDVTAEEAEAIAEEISRAAIEQLAAK
                     GRINFERSAES"
     gene            complement(1116..>2461)
                     /locus_tag="PLC06_15970"
     CDS             complement(1116..>2461)
                     /locus_tag="PLC06_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRO91997.1"
                     /translation="SMGPLNDELKAYTKELQGDIRYDIEWTTLREYLEYLVRRGVSTN
                     VASFVGAGGVRANVLGFADRRPTPDELAQMQTLVRQAMAEGAVGLSAALIYSPDSYAD
                     TAELIALASAAAEYDGLFAAHLRSEGETFLEALEEFLTIVTESGIRGEIYHLKASGQR
                     NWPKLEKAIARVEAARAAGLPVTADMYTYHASATGLDAMMPGWVQEGGHKAWVARLRD
                     PEVRARLKREIALPSADWENSYHEAGDASKVLCVSFKNDALKPLTGKSLAEIAALRGA
                     DPVETAMDLVIEDDSRVGCVYFTMSEENVRREIGLPWVSFCSDSASLAPEGVFLRSNP
                     HPRAYGSFARLLGKYVRDEGLIPLEEAVRRLSALPADNMKLDRRGRLLPGHFADVVIF
                     DPATIQDHATFDRPHQYATGVRDVLVNGVAVLRDGEHTGATPGRVINGPGVKSDK"
BASE COUNT          444 a          760 c          732 g          525 t
ORIGIN      
        1 tccccattct cattcgcctc attctgccca atcgggccaa agtctggctt aagtcgtttg
       61 gcggctggct ggagaaacac aacgaccgga tcgtcatggt cgtgtcgctg gtgtttggct
      121 tattgttcct ttatcagggt ataaccgggt tgtaacggaa ggaaccacct ctgagcgcaa
      181 tcttaaattg gcggagaagc tgattgccga aggccaaatg aggctggcgg ggcgggaggc
      241 gcctggcctg ggtgactagg gtgtcgtagc ggataagaga tcggcaaagg ctacggtcga
      301 aacgatcttg actctcgcga tctcctccag caccagcagg tctttatctc ccgtcaccag
      361 ataatcggcc tgtccgacaa cggcataagt caggaggtaa tcgtctttgg cgtcgcgggc
      421 gatcgcgggg attgcctgct caattcggag gattccttcg gccactgttg ttaacagttt
      481 gagaaatgtc gttagcttgt ccgatccaac ccgccccatc aaatgtgggc gattggcaat
      541 aacatccaga atctcatcca caacctcgtc gggtaatagc aatgtgaatt ggttccggcc
      601 gagtgcctcg aaaacaacct tcaccgagcc ggcgtgctgc ggtgtgagca aatagctgat
      661 aaagacgttg gtgtcgagca gcgccctcat gattcggccg atcgctcaaa gttgatccgg
      721 cctttggctg ccaattgctc aattgccgcc cgggagattt cttcggcgat ggcttcggct
      781 tcttcggccg ttacgtcttc gcttgctttc tgcatctcgc gggcgatttc ctgcaattgg
      841 gtaattgcct gagtgcggcg aatgttctcc ttggcctgtc taagctcctg gtactcggca
      901 aagggaacaa tgacaacctg cggatacccg cggctttgga cgaccacgct gtcaccggtc
      961 ttcacggccc acttcatgag atcgcttaat tttgccttgg cttcggtagc tgatactgtt
     1021 ttttgcatat gatcccccac ctgcccttat tatggtaatt ttgaaagtca tttcagcggt
     1081 cattataatc gctaatttgc tggtcgtcca gccatctact tgtcagattt cacccccggc
     1141 ccattgatca ctcgccccgg cgtcgccccc gtatgctcgc catcgcgcag cacggccacg
     1201 ccgttgacca gcacatcgcg cacaccggtg gcgtactggt gcggccgatc gaacgtggca
     1261 tgatcctgaa tcgttgccgg gtcgaagatg accacgtcgg cgaaatggcc gggcagcagg
     1321 cggccgcgcc ggtcgagctt catgttgtcg gcagggagcg ccgaaagccg ccgcaccgcc
     1381 tcctccagcg ggatcaaccc ctcgtcgcgc acatacttgc ccagcagccg ggcaaagctg
     1441 ccataggctc gcgggtgggg gttggagcgc aggaagacgc cctcgggcgc cagcgaggcc
     1501 gaatcggagc agaagctgac ccacggcagg ccgatctcgc gccgcacgtt ctcttccgac
     1561 atggtgaagt agacgcaacc gacgcggctg tcgtcctcga tgaccagatc catggccgtc
     1621 tccaccgggt cggctccacg cagggcagcg atctcggcca gcgacttgcc ggtcagcggc
     1681 ttgagcgcgt catttttgaa gctgacgcac agcaccttcg acgcgtcgcc tgcctcgtgg
     1741 taggagttct cccaatcggc gctgggcagg gcgatctcgc gcttcaggcg cgcgcgcact
     1801 tcggggtcgc gcaggcgggc gacccacgcc ttgtgaccgc cttcctgcac ccagccgggc
     1861 atcatcgcgt ccagcccggt ggccgaagcg tggtaggtgt acatatcggc cgtgacgggc
     1921 aaccctgcgg cgcgcgctgc ttccacgcgg gcgatggcct tctccagctt gggccagttg
     1981 cgttgccccg acgccttcag gtgatagatc tcgccccgta tgccggattc ggtgacgata
     2041 gtcaggaact cctccagcgc ttcgaggaag gtctctccct cgctgcgcag atgggcggcg
     2101 aacaatccgt cgtattcggc cgcagccgaa gccagcgcga tgagttcggc cgtgtcggcg
     2161 tagctgtcgg gcgaatagat gagcgccgcc gagaggccga ccgcaccctc ggccatcgcc
     2221 tgtcggacga gagtctgcat ttgggctagc tcgtcgggcg tcggccgccg gtcggcgaag
     2281 cccagcacgt tggcgcgcac gccccccgcg ccgacgaatg aagcgacatt cgtcgatacg
     2341 ccccgacgaa ccagatactc caggtactca cgcagggttg tccactcgat atcgtagcgg
     2401 atgtcccctt gcagctcctt cgtgtaagcc ttgagttcgt cgttgagcgg tcccatcgac
     2461 c
//
LOCUS       DAOMQG010000462         2441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17220_length_2441_cov_10.124895, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000462 DAOMQG010000000
VERSION     DAOMQG010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17220_length_2441_cov_10.124895"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..830)
                     /locus_tag="PLC06_15975"
     CDS             complement(270..830)
                     /locus_tag="PLC06_15975"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRO91998.1"
                     /translation="MVLVIDNYDSFTYNLVQYLGELGEDVRVYRNDRITLEEIAALKP
                     DHIVISPGPGTPDDGGISNEVIRAFGPTTPVLGVCLGHQCIGHVFGGVVSRAPRLMHG
                     KTSSVYHNGHGLFNGVPSPFTATRYHSLIVEEAMPESLAVTAFTRDGEVMALQHKEYP
                     VVGVQFHPESILTEHGKRILQNFLSM"
     gene            complement(925..1857)
                     /locus_tag="PLC06_15980"
     CDS             complement(925..1857)
                     /locus_tag="PLC06_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO91999.1"
                     /translation="MKRFTLLLILAALLAACDNAPQDTAASLPTPLPPVPTADTTTGQ
                     QPTATGAALATLPISPTAEPSTELPTLTPLATDAPPPSPEVSPTVESPTAEPTAVSLF
                     APGQQDGGSLAAGQATAYLVDARRFQPLILFVEPTNELNIALAAYVGDQTQQTMPEGV
                     TPPVAADNTMAGRPEILVLSPEADGLYTFVVRAVAGEGSFAVRLYDMTTPASGVAVQQ
                     PDALAAGEAKSYTVTSRGPRPVIAAADPTDQSDLALDILGADGTLLTTANYSGPGGVE
                     TAYVLPLGETTYTVRIREANGGPAAFQILVITLE"
     gene            complement(2106..>2441)
                     /locus_tag="PLC06_15985"
     CDS             complement(2106..>2441)
                     /locus_tag="PLC06_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRO92000.1"
                     /translation="AVTDSGREIRFSDDPEKAGVNNLLGIYQAVTGKDRVAVEADFAN
                     ARGYGDLKVGVAEVVIEALQPIQARYRELMDDAAELDRLLAYGADQARAVSQPKVDEM
                     KRIMGLVVP"
BASE COUNT          459 a          746 c          739 g          497 t
ORIGIN      
        1 tcggtaacat cgagtggctt tgccattttc aaatatctcc ttcgagttct ctattattct
       61 tcctgcggcc aaccatggat tgtagttaac cttcaagcta gtagacgcag tgtttccgtt
      121 gattcttact tggtcaacct tgcaattgag caagcgtgag tcggattata caagaccggc
      181 aataactttg catccaacca attctgaccc gcaacgcagc ctgccgaatc gcggcgaagc
      241 aagcgagttc cacgccatcc tggacttcct tacatgctca gaaaattctg caatatccgc
      301 ttgccgtgct cggtcaggat gctttcgggg tggaactgga cgccgacgac ggggtactcc
      361 ttgtgctgca gggccatcac ctcgccgtca cgggtgaagg cggtgacggc cagcgattcc
      421 ggcatcgctt cctcgacgat cagcgagtgg tagcgcgtgg ccgtgaaggg gctggggaca
      481 ccgttgaaca ggccatggcc gttgtggtag accgacgagg tcttgccgtg catcagccgc
      541 ggcgcgcgcg ataccacgcc gccaaagacg tggccaatgc actgatggcc gaggcaaacg
      601 ccgagcaccg gcgtggtcgg cccaaaggcg cggatcactt cgttggagat gccgccatcg
      661 tcgggcgtgc cggggccggg gctgatgacg atgtggtccg gcttgagcgc ggcgatctcc
      721 tccagcgtga tccggtcgtt gcgatagacg cgcacgtcct cgcccagctc gcccagatat
      781 tgcacgaggt tgtaggtgaa tgaatcgtag ttgtcgatga ccagaaccat ctgtctctcc
      841 tgcttgtata gatcgaccac ggactacaga ccacggacca cgaatatcat ttgattgtca
      901 tccgccgcct gtcgtccgac atcattattc caatgtaatc accagtatct gaaacgccgc
      961 cgggccgccg ttggcttcgc ggatccgcac ggtgtaggtc gtctcgccca gcggcagcac
     1021 ataggccgtc tctacgccgc ccggcccgct gtagttggcc gtggtcagca gcgtgccgtc
     1081 cgcgcccagg atatcgaggg ccaggtctga ctgatcggtc gggtcggcgg ccgcgatgac
     1141 cggccgcggc ccgcgggatg tgacggtgta actcttcgcc tcgcccgcgg ccagcgcgtc
     1201 gggctgctgc acggcgacgc ccgatgcggg tgtcgtcatg tcatagaggc gcacggcgaa
     1261 ggagccttct ccggcgaccg cccgcaccac aaacgtgtag aggccgtcgg cctcagggct
     1321 gagcaccaga atctccggcc gcccagccat cgtattatcg gccgccacgg ggggcgtgac
     1381 cccttccggc atcgtctgct gcgtctggtc gcccacgtag gcagccaggg caatattcaa
     1441 ttcgttcgtc ggctcgacga acaggatgag cggctggaag cggcgcgcat cgaccagata
     1501 ggccgtcgcc tgtcccgctg caagcgagcc gccgtcttgc tgaccggggg caaacaggct
     1561 gaccgccgtc ggctcggccg tgggcgattc gacagtgggc gatacctcag gcgatggtgg
     1621 tggcgcatcc gtcgccaaag gcgtgagcgt cggcagctcg gtggatggct cggccgtcgg
     1681 cgatatgggc agcgtggcca atgcggcccc ggtggccgtc ggttgttgtc cggttgtcgt
     1741 gtcggccgtc ggtacgggtg gcaatggtgt gggcagtgaa gcggccgtat cctggggcgc
     1801 gttgtcgcac gccgccagca gggccgccag aatgagtaac agagtgaatc ttttcatcaa
     1861 ataccccctt atttcaggag acgcaaaaaa gcagccgcga tcgaaatggg tgcagctcct
     1921 tcactctatt gtaccccgct tgacgcgcca tcctatggga ctatttacca agatatcacc
     1981 tgaggaatca ggaattagga attacgaatg aagagcatgg gtgatgaatg acttaatcct
     2041 tcgcccgatg acaagcaatc acccgtacgc gccctcttca attcctaatt cgcaattcgt
     2101 aattgttaag gtacgaccag ccccatgatt cgcttcatct cgtccacttt gggctgcgac
     2161 acggcccgcg cctggtctgc gccataggcc agcagtcgat cgagttcggc agcatcatcc
     2221 atgagttccc ggtagcgcgc ctgaatcggc tgcaatgcct caatgaccac ctcggccaca
     2281 ccgactttca ggtcgccata gccgcgcgca ttggcaaaat ccgcctctac ggcgacgcga
     2341 tccttgccgg tcacggcctg atagatgccc agcagattgt tgacccccgc cttttcggga
     2401 tcatcggaga agcgaatctc gcggccgctg tcggtgacgg c
//
LOCUS       DAOMQG010000463         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17265_length_2436_cov_9.045359, whole genome shotgun sequence.
ACCESSION   DAOMQG010000463 DAOMQG010000000
VERSION     DAOMQG010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17265_length_2436_cov_9.045359"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="PLC06_15990"
     CDS             complement(<1..1574)
                     /locus_tag="PLC06_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HRO92001.1"
                     /translation="MKTTNGKQLQFTPKSTTARQGNLLLLATLLVAVLMIAAGIGRAR
                     ADVDGETMTRERAAPTALPLPTTKEDFFLPGTQPGSDAHPLVEIADPDSCSACHTEAI
                     YDTWRGSMMAQAGRDPVFWAAFAVAQNDAADAGEYCLRCHTPRGWYAGRSEPADGSAL
                     EATDISAGVACELCHRMVDPIPGANDEVAAIDTAIRSALTDPPPGDHFGSAMIILDPE
                     DRRRGPFSFPPSPEIGPHHRWQSRFQGQNDALAASRLCGSCHNVDNPLLSWNENPPGG
                     GPAQYWPNDMNTAAPSFGKDMLFPIERTYEEWLYSAYANGGVHAPQFAGAKPDGIVAS
                     CQDCHMVRSTGIAAETFFNPVYRNCNTTGCLPVHVLAGGNTWVPKIIQDERWRLNPPA
                     FEATALNATIAASQSMLQRAATLDIDLEEDDGQKVVTVRVINETGHKLPTGYPEGRRL
                     WINLRAYNAAGALVYESGAYNPTTGILTEDAAIKVYEVEQGLTPELAALTGLDAGPTF
                     HFALNNTTYKDNRIPPR"
     gene            complement(1571..>2436)
                     /locus_tag="PLC06_15995"
     CDS             complement(1571..>2436)
                     /locus_tag="PLC06_15995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRO92002.1"
                     /translation="GGAAIFVGAILGVLIIYGGDPQPTVIGVVLAASIVALTGLIDDY
                     RPLPAWMKLSGQFLGFLILAYFGIRVRLPVPDFVNYAITLLWLLGITNAINFLDNMDG
                     LSAGISAVMASFILLLALINGQFLVAALAAGLLGACLGFLRYNFPPAQIFMGDVGSLF
                     LGFLLAVMGIQLRFPANSNFVTWMVPVFLFGLPIFDMMLVVSSRLRRGLSPNTAGKDH
                     ISHRLVLLGFSSREAILILYLFSGALGMMALYITQATVIEAYAIALVAVILAVAAIIR
                     LERKWMESESP"
BASE COUNT          527 a          684 c          763 g          462 t
ORIGIN      
        1 cggggcggga tgcgattgtc cttgtaggtc gtgttgttga gcgcaaagtg gaaggtcggc
       61 cccgcgtcga gacccgtcag cgcggcaagt tccggcgtca gcccctgttc aacctcgtag
      121 actttgatcg ctgcgtcctc ggtcaggatg ccggtcgtcg gattataggc accggactca
      181 tagacgagag cgccggccgc attgtaggct cgcagattga tccacaaccg ccgtccttca
      241 gggtagcctg tgggcaattt gtggccggtt tcgttgatga cccgaacggt gacgaccttt
      301 tgtccgtcat cctcttcgag gtcgatatcc aacgtagcgg cgcgctggag catgctctga
      361 gaggcggcga tcgtggcgtt cagcgccgtg gcctcaaatg caggaggatt gagtcgccag
      421 cgctcatcct gaatgatttt gggcacccag gtgtttccac cggccagcac gtgcaccggc
      481 aggcagccgg tggtgttgca gttgcgataa acggggttga agaacgtttc cgcggcaatt
      541 ccggtgctgc gcaccatgtg gcaatcctgg caggaagcga ctatgccgtc gggcttcgcc
      601 ccggcaaatt gtggagcatg gacaccaccg ttggcataag cgctgtagag ccattcctcg
      661 taggtgcgct cgatggggaa gagcatatcc ttgccgaatg atggcgcggc cgtgttcatg
      721 tcgttgggcc aatattgagc cggtccacca ccgggcgggt tctcattcca gctgagcaat
      781 gggttgtcca cgttgtggca actgccgcag agccgggaag cggccaaggc atcattctgc
      841 ccctggaagc gagattgcca gcggtggtga gggccgatct cgggcgaagg cgggaaggag
      901 aaaggccctc gccgcctgtc ttccggatca aggatgatca tggccgagcc gaaatggtcg
      961 cctggtggcg gatcagtcag agcggaacgg atggctgtat caatagcggc cacttcatca
     1021 ttggctccgg ggattggatc gaccatgcgg tggcaaagct cacacgcaac accggcgcta
     1081 atgtcggttg cctccagcgc gctgccgtcg gccggttcgc tgcgccctgc ataccaacca
     1141 cgcggtgtgt ggcagcgcaa gcaatattca ccggcatcag ccgcatcatt ttgagcgacg
     1201 gcaaaggccg cccaaaagac gggatcacgt ccggcctgag ccatcatgct gccccgccag
     1261 gtatcataga tcgcctcggt atggcaggcg ctacaggagt caggatcggc gatctcgacc
     1321 agtgggtggg cgtcggagcc gggttgcgtg ccgggcagga agaaatcttc ctttgtggtt
     1381 ggcagaggca gggcagtggg tgcagcccgc tctcgcgtca ttgtctcccc atccacgtcg
     1441 gctcgcgcgc ggccgattcc cgcagctatc atcaggacag cgacaagcaa tgtcgccaac
     1501 aatagcagat taccttgcct ggctgtcgtg gattttggcg tgaattgcaa ctgcttccca
     1561 ttcgttgttt tcatggtgat tcactctcca tccattttcg ctccagccgg atgatcgcgg
     1621 ccactgcaag gatgaccgct accagagcga tcgcataggc ctcgatgacc gtggcctgcg
     1681 tgatatataa ggccatcata cccaaagcgc cgctaaacag gtagaggatc aaaattgctt
     1741 cgcgggacga aaagccaagc aatacaagcc gatggctgat gtggtccttg ccggcggtgt
     1801 tcgggctaag ccctcgccgc agccgtgagg atacgaccag catcatatcg aaaatgggca
     1861 gaccgaagag aaaaaccgga accatccagg tgacaaaatt ggagttggcg ggaaatcgta
     1921 actggatgcc cattacggcc agcaaaaagc ccagaaatag ggagccgaca tcgcccatga
     1981 agatctgtgc cggtgggaaa ttgtagcgca agaagcccag gcacgcgccc aaaagcccgg
     2041 ccgccagcgc ggcaaccagg aattgaccgt tgatgagcgc caataacagg ataaatgagg
     2101 ccatgaccgc cgagatgccc gcgcttagcc catccatgtt atcgaggaag ttgatggcgt
     2161 tggtgatgcc gagcagccac aggagcgtga tcgcgtaatt gacgaaatcg ggcaccggca
     2221 ggcgaacgcg aatgccaaaa taggccaaaa tgaggaagcc aaggaattgc cccgacaact
     2281 tcatccaggc cggcaggggg cgatagtcgt cgatgagtcc tgtgagggcg acgatgcttg
     2341 cagccagaac gacgccaatg actgtcggct gtggatcgcc accatagatg atcagcacgc
     2401 cgagtatggc gccgacgaaa atggccgcgc caccca
//
LOCUS       DAOMQG010000464         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17266_length_2436_cov_7.821084, whole genome shotgun sequence.
ACCESSION   DAOMQG010000464 DAOMQG010000000
VERSION     DAOMQG010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17266_length_2436_cov_7.821084"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..784
                     /locus_tag="PLC06_16000"
     CDS             74..784
                     /locus_tag="PLC06_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003780993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO92003.1"
                     /translation="MIPRRILIVDDEPQLQKTIRAYLEREGYAVESAADGHAALDAAA
                     AFRPDLVILDVMLPGLGGFTVLESLRRQSDVYVLMLTARADEIDKVMGLTLGADDYMT
                     KPFSPRELVARVRAILRRERSAPGDAAAVPDEVALAFARLRLEPAARRVWKDEREVEL
                     TPIEYDLLYTLARHAGRVLSREQLIEHVWGYDYYGDDRIIDTHIRRLRRKIEDDPATP
                     AFVVTVRGTGYRFEGRPK"
     gene            781..1788
                     /locus_tag="PLC06_16005"
     CDS             781..1788
                     /locus_tag="PLC06_16005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO92004.1"
                     /translation="MKSLLSLHRRVAWLHLFSYLVIVLIELIVLRNLSGTLEANVVRY
                     LTFFAVVLGSLLVIRRRIIRPIEEMKEASLRIAAGNYQERLPTYSTIELNELAQAFNR
                     MVATIESTEERRIMLLSDVAHELRTPLSNIRATMEGLIDEVLPADPDTFFDIQREVGR
                     LQRLVQQLDALSRAESDRLPLNKQPADIAALAQRVCDRLSIQYEDKEVALELEAADDL
                     PPLSVDPDLITQVLTNLLGNALQYTPRGGQVRVIVARQRGDILIRVADNGIGIPAEDL
                     TRIFERFYRVDKSRARQSGGNGIGLTIAAHLVRAHNGRIWAESDGPGHGATLSFTLPL
                     Q"
     gene            1912..2301
                     /locus_tag="PLC06_16010"
     CDS             1912..2301
                     /locus_tag="PLC06_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92005.1"
                     /translation="MDDLFVSPAVHMMVGAVVLLTGLAAMVVAVRAAMKRQPAGRNGN
                     LLFVLFQLALMVQVLIGIKLLDQGLGPLQLFVHYLGGLAPMGFVLLYYWLRPADGVGQ
                     GRRAAVVTVASFMFVLMTFTIGSMYVA"
BASE COUNT          430 a          755 c          749 g          502 t
ORIGIN      
        1 tggtcatcga agggaccgcc caataccagg cctacgcggc cgccgtgcgc gccgcgatcc
       61 gcacaatcga atcatgattc cacgccgtat tctcatcgtc gacgacgaac cgcagctgca
      121 aaaaacgatc cgcgcctatc tggagcgtga agggtacgcg gtggaatcgg ccgccgacgg
      181 ccacgccgcg cttgacgccg cggccgcctt ccggcccgac ctggtgattc tcgatgtcat
      241 gttgcccggc ctgggcggct tcaccgtgct ggagtcgttg cgccggcaat ccgacgttta
      301 cgtgctgatg ctgaccgccc gcgcggacga gatcgacaag gtcatggggc tgacattggg
      361 cgccgacgac tacatgacca aacccttcag cccgcgcgaa ctggtcgccc gcgtgcgggc
      421 catcctgcgc cgggaacgga gcgcgccggg cgacgcggcc gccgtccccg acgaggtggc
      481 gcttgccttc gcccggttgc gccttgagcc ggccgcccgc cgggtgtgga aggatgagcg
      541 cgaggtcgaa ctgacgccca ttgagtatga cctgctctat acgctggccc gccacgccgg
      601 gcgcgtcctc agccgcgagc agctcatcga acacgtctgg ggctacgact actacggcga
      661 cgaccgcatc atcgacaccc acattcgccg cctgcggcgc aagattgagg acgatccggc
      721 cacgcctgct ttcgtcgtca ccgtgcgcgg cacgggctac cgtttcgaag ggcgcccgaa
      781 atgaaatcgc ttctgtcgct ccatcggcgc gtcgcctggc ttcatctgtt ctcctatctg
      841 gtcatcgtcc tgatcgaact catcgtcctg cgcaacctca gcggcacgct ggaagccaac
      901 gtcgttcgtt acctgacctt cttcgccgtc gtcctgggca gcctgctggt catccgccgg
      961 cgcattatcc ggcccatcga ggagatgaag gaggccagcc tgcgcatcgc cgccggaaac
     1021 tatcaggagc ggttgcccac ctattccact atcgaactta atgagttggc ccaggcgttc
     1081 aaccgcatgg tcgctaccat tgaatccacc gaagagcggc gcatcatgct gctgagcgat
     1141 gtggctcacg aactgcgcac gccgctcagc aatatccggg ccacgatgga agggctgatc
     1201 gacgaagtgc tgcctgccga tccggatacg ttcttcgaca tccagcggga agtcggccgc
     1261 ctgcagcggc tggttcagca gctggatgcc ctgtcgcggg ccgagagcga ccggttgccg
     1321 ctcaacaagc agccggctga tattgccgcg ctggcgcagc gtgtttgcga ccggctatcc
     1381 attcaatatg aggataagga agtagcgctg gagctggagg cggccgatga tttgccgccg
     1441 ctgtctgtcg atcctgacct gatcacccag gtgctgacca atctgctggg taacgcgctg
     1501 caatatacgc cgcggggcgg gcaagtccgt gtcatcgtcg cgcggcagcg gggcgacatt
     1561 ctcatccgcg tagcagataa cggcattggc ataccggctg aagacctgac ccgcatcttc
     1621 gaacgcttct atcgcgtcga caagtcgcgg gcgcggcaaa gcggcggcaa cggcatcggg
     1681 ctgactatcg ccgctcatct cgttcgcgcc cacaacggcc gcatctgggc cgagagcgac
     1741 ggtccggggc acggggccac gctcagcttc acgttgccgc ttcaatgacc aagccgatag
     1801 ggcgggccaa aattgtcaca caatcgtcat attacggaca tccatttgcc atcttgcccg
     1861 ggtagaatcc gggttgtagc acttaatatt ttcaaggaga tttgtattct gatggacgat
     1921 ttatttgtat ctccggccgt tcatatgatg gtcggggctg tggtgttact caccggtctg
     1981 gcggcaatgg tggtcgcagt acgcgccgcc atgaagcggc agccggccgg ccgaaacggt
     2041 aacctgcttt ttgtcctgtt ccagctggcg ctgatggtac aggttctgat cggtatcaaa
     2101 cttctggatc agggtctggg gccgttgcaa ctatttgttc attatctggg cgggctggct
     2161 ccgatgggct tcgttctgct ttactattgg ttgcgtccgg ctgacggcgt gggtcagggt
     2221 cggcgagcgg ccgtcgttac ggtggcatcg ttcatgtttg tgctgatgac cttcaccatc
     2281 ggcagcatgt atgttgcctg aggtgcaggg taggggcggg tttcaacccc gcccctgtca
     2341 ggaaaccggt ttgtgttggc ctaatctgtc ctgcttgtgt ggagccgggt atcataaact
     2401 tcaattatca attgcgccgg gccgcgaaac gaaatc
//
LOCUS       DAOMQG010000465         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17281_length_2434_cov_8.920135, whole genome shotgun sequence.
ACCESSION   DAOMQG010000465 DAOMQG010000000
VERSION     DAOMQG010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17281_length_2434_cov_8.920135"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..683)
                     /locus_tag="PLC06_16015"
     CDS             complement(162..683)
                     /locus_tag="PLC06_16015"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HRO92006.1"
                     /translation="MNTSERIPKAMAEKFAAIVTLTDAFCAQHLNEEYRQLIHRVVGA
                     LARKRPSPLSSGKESVWAAAAVHAVGRVNFLDDPSQDPHCKPKVIYSFFGIAESTGQN
                     KSKTIRDALKMGQFSHEWILPSRLADNPMAWMLQVNGLMMDIRAAPLELQRLAYEKGL
                     IPFIPAEQAETPD"
     gene            complement(1029..1127)
                     /locus_tag="PLC06_16020"
     CDS             complement(1029..1127)
                     /locus_tag="PLC06_16020"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRO92007.1"
                     /translation="MGGWPGALAAQRRLRHKSSKQSFQVTFWITWQ"
     gene            complement(1608..2375)
                     /locus_tag="PLC06_16025"
     CDS             complement(1608..2375)
                     /locus_tag="PLC06_16025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRO92008.1"
                     /translation="MKLRHWIAAMTVAATLPALAAVESREITLPNDLGSAVLYTSGKA
                     EAGPAVIVVHEWWGLNDYARDRARQLAEAGYTAIAVDMYGHGKVADHPKDAQAFMQAA
                     LAEPEKMGARFNAARAALAAEPNVDATRIYGVGYCFGGAVVLNQARMGADLAGVASFH
                     GSVGTNNPAQAGKVKARIFVATGGADPMIPPEQVGNFVTEMMTAEADLKLISYPGVLH
                     GFTNPGATEVGKTHNMPVAYDEHADQDSWKALMEFIK"
BASE COUNT          404 a          732 c          796 g          502 t
ORIGIN      
        1 cctgatgaac tgcatcaacg ccttcctgct cgcctggtcg gcgtgttcgt cgtaggcgag
       61 cggtatgtcg tgctgctggc cgaatacgtg cggctcattg ccgcgcaggc ctcgccttga
      121 catcgcacgg ccggcccaca tcgatgacga gtccgatcgg ctcaatcggg cgtctccgcc
      181 tgttccgccg gaatgaaggg aatcaggcct ttctcgtagg caaggcgctg cagttcgagg
      241 ggcgcggctc gaatgtccat catcaagccg ttgacctgca gcatccatgc catcgggttg
      301 tcagcgagcc ggctgggcaa gatccattcg tgcgaaaact gccccatctt cagtgcgtcc
      361 cggatcgtct ttgacttgtt ctgcccggtg ctctcggcga ttccgaagaa cgagtagatg
      421 accttcggct tgcagtgcgg atcctgcgag gggtcgtcga ggaaattcac ccgcccgacc
      481 gcatgcacgg cagcagcagc ccagacgctc tcctttccgc tcgacagcgg tgaagggcgt
      541 ttgcgggcga gggcaccaac aacgcggtgg ataagctgac gatattcctc gttgaggtgc
      601 tgcgcgcaga atgcgtcggt aagggtgacg atggcagcaa atttttctgc catggccttt
      661 gggattcgct cggacgtgtt catggtggta acaagcacgg aagacgcaac attgtcacag
      721 attggccggc cgacaccaac gggccagcca cctttatgta aggagcaaat aaactgtccg
      781 gagttgtagc acgtgatctg tccggttttc attgagcccc gaggaggggt tcttcggctt
      841 ccggttgctg aagcgttatg gccgcgtcac cagattcttc gaaggcatcg accgccgcat
      901 ctttctggag aattaatccg cgctcagccc cttttctcct gttttccagc cgtttcaggt
      961 caagtccagc aggctacgaa gcattgagtc gccgtcggac atcatcagcc agcctagagc
     1021 gccgccgttc attgccacgt tatccagaac gtcacctgga acgattgctt gctggacttg
     1081 tggcgcagcc gtctttgggc ggccagcgca ccgggccagc cgccgatgag cgcgaatgca
     1141 cgcagcgtgc tttcctgagt gcgctatgcg ccgcgtggga tagggtggca cccatgagcc
     1201 agcccctttc ccttcgttcg cgcgcatcag cggatctgaa ctgcgcctcc cccgctggcg
     1261 cgcgggcgag agtaccctga cccggtactt gcagtgccgg ccggcgaacg atcgcgcgac
     1321 cttagaaccg acggtattcc atgtccgcct cctcgggccg gggccgtgca gctcgaaggg
     1381 aagaagacca gatgctggcc gcgttgtctc atggcgcgaa atgccgcgct cggacctgtt
     1441 tgcgggtcat aggaaatgtc aaagggtggg cggtcgctct gcgatatcag ctggccgcca
     1501 cgatagagct gatagcactc cgcaagaacc ggagatgcgc tcagcatcag tgcaagtgcc
     1561 attagtgctt tcatcatgtt cctccttgcg cctgccggcg cggcccctta cttgatgaac
     1621 tccatcaacg ccttccagct gtcctggtcg gcgtgttcgt cataggcgac gggcatgttg
     1681 tgcgtcttgc cgacttcggt ggcgcccggg ttggtgaagc cgtggaggac gccggggtag
     1741 ctgatgagtt tgaggtcggc ttcggcggtc atcatctcgg tgacgaagtt gccgacctgc
     1801 tcgggcggaa tcatcgggtc ggcgccgccg gtggcgacga agatgcgcgc cttgaccttg
     1861 ccggcctggg cagggttgtt ggtgccgacg ctgccgtgga agctcgcgac gccggcgaga
     1921 tctgcgccca tgcgggcctg attgagcacc accgcgccac cgaagcaata gcccacgccg
     1981 tagatgcggg tggcgtcgac gttgggttcg gcggcaagcg cggcgcgcgc ggcgttgaag
     2041 cgggcgccca tcttctcggg ctcggccagc gcggcctgca tgaaggcctg ggcgtccttg
     2101 gggtggtcgg cgaccttgcc gtggccgtac atgtcgaccg cgatcgcggt gtagccggcc
     2161 tcggcgagct ggcgggcgcg gtcgcgggcg tagtcgttca gaccccacca ttcatgcacg
     2221 acgatgacag ccgggccggc ttcggccttg ccggaggtgt acaggacggc gctgcccagg
     2281 tcgttcggga gcgtgatctc gcgggattca acggcggcca gcgccggcag cgttgcggca
     2341 acggtcatgg cggcaatcca gtgacgtagc ttcatggtga ttctcctctc tgttgttgtc
     2401 ggggtgacac gcttgttatc gattggaacg gaag
//
LOCUS       DAOMQG010000466         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17302_length_2432_cov_9.264199, whole genome shotgun sequence.
ACCESSION   DAOMQG010000466 DAOMQG010000000
VERSION     DAOMQG010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17302_length_2432_cov_9.264199"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PLC06_16030"
     CDS             <1..1320
                     /locus_tag="PLC06_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO92009.1"
                     /translation="ATIASRRAYLAQEAALARTETALADQARLSGELRAVSDVSVAAA
                     ATLDVDRLLAAASDLTKENFNLYHTHIYLLDDDRKTLALRAGAGEVGREMVREGRIIP
                     LGERSIVARAARERDVVLVSDTRRSTDFLPHLLLPDTRSEMAVPLIIGDRLLGVLDVQ
                     SNQSGYFRPEDRQVYKILASQLAVATQNALYFGEQLEMAEKLREVDRLKTDFLARMSH
                     ELRTPLNSIIGFADVLLLGLDGDLTERMIEDLELIRGGGYHLRDIISDILDMSKIEAG
                     RLELVYEEFDARRIASELMATAAPLAEQKGLALRLEISEGVEPITADRTRVRQVLWNI
                     IGNAIKFTDHGSIDVAICPDDDGVLFAVTDTGIGISPADQERIFEYFSQVDAGRRESI
                     SGTGLGLSISKSLVEYHGGRIWVESEPGRGSTFRFTIPRQAVVNGVL"
     gene            1394..1762
                     /locus_tag="PLC06_16035"
     CDS             1394..1762
                     /locus_tag="PLC06_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO92010.1"
                     /translation="MTRKRVLCVEDNDSNMRLVSRIVEGERHEFLAAVDGTMALAMAR
                     REQPDLILLDINIPGMDGLELARHLKGDATLSVIPIIATTANVLHGDRERCLDAGCDA
                     YLPKPLDVRELQLLLRGYLR"
     gene            2052..>2432
                     /locus_tag="PLC06_16040"
     CDS             2052..>2432
                     /locus_tag="PLC06_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92011.1"
                     /translation="MKRLAIILVVGAAALLSVLNAQAQPALLPIGAIQGMGDVSPYLN
                     RFVNFRGVVVGRYEDVNTRGDVYYTLFVQDIPGAGDADPATSDGIAVFLGRQPRPDIS
                     LGAVVTVGGKVTEFYGLTEIDDKGL"
BASE COUNT          472 a          677 c          791 g          492 t
ORIGIN      
        1 gctactatcg ccagccgccg cgcctatttg gcgcaggagg cagcgctggc ccgcaccgag
       61 acggcgttgg ccgaccaggc gcgattgtcg ggcgagctgc gagcggtgtc ggatgtcagc
      121 gtggccgcgg ccgccacgtt ggacgttgac cggttgctgg cggccgcgtc tgacttgacg
      181 aaagagaact tcaacctcta ccacacccac atctacctgt tggacgacga tcgaaagaca
      241 ctggcgctgc gggccggggc gggtgaggtt ggccgcgaga tggtgcgcga gggtcggatc
      301 attccgctcg gcgagcgctc catcgttgcc cgggcggcgc gcgagcggga cgtggtgctg
      361 gtgagcgata cgcggcgctc gaccgacttc ttgccacacc tgttgttgcc cgacacgcgc
      421 tcggagatgg ccgtcccgct catcatcggt gatcgattgc tgggcgtgct ggacgttcaa
      481 tcgaaccagt caggctattt tcgccctgaa gaccggcaag tatacaagat tctggcctcg
      541 cagttggctg tggcgactca gaacgcgctc tacttcggcg aacaactgga gatggccgag
      601 aagttgcgcg aagttgaccg gttgaaaacc gacttcctgg cgcgcatgag ccatgagctg
      661 cgcaccccgc tgaactcgat catcggcttt gccgacgtgc tgcttctggg tctggatggt
      721 gatctgaccg agcggatgat tgaggatctg gagctgattc gcggcggagg ttaccatctg
      781 cgggacatca tcagcgacat tctggatatg tcgaagatcg aggcagggcg gctggaattg
      841 gtctatgagg agttcgacgc gcgacgaata gcgtctgaac tcatggccac cgccgcgccg
      901 ctggccgagc agaaggggtt ggcgctgcgg ctggagatct ccgagggagt tgaaccgatc
      961 acggccgacc ggacacgcgt gcggcaggtg ctgtggaaca tcatcggcaa cgcgatcaag
     1021 ttcaccgacc acggcagcat cgatgtcgcc atctgtcccg acgacgatgg tgtgttgttt
     1081 gccgtgaccg acacaggtat tggcatcagc ccggcggatc aggagcgcat cttcgaatac
     1141 tttagccagg tggacgcggg gcggcgggag tcgatcagcg gcaccgggct ggggctatca
     1201 atcagtaaga gtctggtgga atatcacggc ggccgcatct gggtagagag cgagccgggg
     1261 cgcggcagca cgttccggtt cacgatcccg cgacaagcgg tcgttaatgg tgtgttgtaa
     1321 ttttgtaatt aactttcagg cggttcaatt aggatcaggc ttagccaaaa cgggacgtgt
     1381 gaggaaaaag cggatgacgc gaaagcgagt tctgtgcgtt gaagataacg atagtaatat
     1441 gcgccttgta tcgcgcatcg tggaagggga gcggcacgag ttcctggcgg cggtcgacgg
     1501 cacaatggcg ctggcgatgg cccggcgcga gcagcccgat ctgatcttgc ttgacatcaa
     1561 tatccccggt atggatgggc tggaactggc tcgacatttg aaaggggatg cgacattgtc
     1621 cgtcataccg atcatcgcca cgacggccaa cgtactgcac ggcgaccggg aacgctgcct
     1681 cgacgcgggc tgcgatgcct atctgcccaa gccgctggac gtgcgggagt tgcagttgct
     1741 cttgcgagga tatctacgat aactctgcat tacaggaatc ctctgaatat ttggctgcct
     1801 aaataacctg cgctgcttcg tagttaggcg cttcagcgtc gctccgggga cgcaactatt
     1861 gcattttacg aaataatcac gtctcgcttc cgggcgggat caatcccgcc gctgacagac
     1921 aaaaccgact caagtcggtt aacgcctctg tcggaacgtt tcgccggcca caaaattcgc
     1981 gtgattatta attgggtaac gtcccggact gtccgtgcaa gctgcgaggg aacccggaga
     2041 ctgggagacg cgtgaaacgg ctcgctatta tccttgttgt tggtgcggcc gctttgctgt
     2101 cggtgctcaa tgcacaggcc caaccggcat tgctgcccat tggcgctatc cagggcatgg
     2161 gcgacgtatc tccctatctc aaccgcttcg ttaactttcg cggcgtggtc gtcggccgct
     2221 atgaagacgt aaacacccgc ggcgatgtct attacaccct gtttgtccag gatatccccg
     2281 gcgcgggcga cgccgacccg gccacatccg acggcatcgc cgtgttcctg gggcgacaac
     2341 cgcgccccga catctcgctt ggcgcggtgg tgacggtcgg cgggaaggtg acggagttct
     2401 atgggctgac ggaaatcgac gacaaagggc tg
//
LOCUS       DAOMQG010000467         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17341_length_2425_cov_6.645570, whole genome shotgun sequence.
ACCESSION   DAOMQG010000467 DAOMQG010000000
VERSION     DAOMQG010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17341_length_2425_cov_6.645570"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLC06_16045"
     CDS             <1..713
                     /locus_tag="PLC06_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92012.1"
                     /translation="NLLGDVKFAGVLWASILAIAFCSIDFAGLVRIFTPQKGLEESRA
                     VLFMTGAWFLGTIANSVTTWWAVSLTLLSHEFGNEVLSREMLLRYVPIFVAMLVWLTR
                     ILFIGAFSVAGGDVFELTRSQPGNKPGATPRPDLPVGAPVRAASAQPASYQAATQPAA
                     QARNPQHQTRQYPSERSTITYEPVDEPIVGQPTMPRTNGGAPSHSTSRIQQRPPMPGM
                     GHSPAAGPVQARSSKPGH"
     gene            783..1550
                     /locus_tag="PLC06_16050"
     CDS             783..1550
                     /locus_tag="PLC06_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRO92013.1"
                     /translation="MNQSNDDEVWQFPPGYGQPMGNPMATVQRRMMLILVAATLFITA
                     FGFSVIRVSNIGTTTNDSTTTDEAAAQSQAAATASAEEQPIAPVAATGGIAPLFTPEI
                     QHWAPQIVAWANAAGIDPNMAATVMQIESCGDPNAVSGAGAQGLFQVMPFHFDPGEAM
                     QDPDTNARRGMAYLALGMQQTGGDTSRTFAGYNGGHGTAARSWDTWPAETQRYYVWAT
                     GIYGDAVNGSATSETLNQWLTAGGAGLCQQAAARLGL"
     gene            1701..2312
                     /locus_tag="PLC06_16055"
     CDS             1701..2312
                     /locus_tag="PLC06_16055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO92014.1"
                     /translation="MLESELYEARNYDRGMWLLERAKLSELRQRVVQVVTGNTLEIGA
                     GTGANVAHYRDDVTVTAIDLRPTHIMTAGDKARRRHPGFEFTAACADAQWLPFSDNTF
                     DTVLGTLVFCSIARPQDALLEIQRVLRPGGQLILLEHVRGRTPFTRRLTDWLHPLWFA
                     AQGVCHLNRETATTVAETGFRIDYTSQHARGLIQVIHATSPKT"
BASE COUNT          520 a          759 c          670 g          476 t
ORIGIN      
        1 acaacttgct gggggatgtc aagtttgccg gagtcttatg ggcgtccata ttggctatcg
       61 ccttttgctc catcgacttc gccggtcttg tccgcatctt cactccacaa aaagggcttg
      121 aagagtccag ggcggtccta tttatgaccg gggcatggtt tctgggcacc atcgccaact
      181 ccgtcaccac ctggtgggcc gtatccttga ccttgctcag tcatgaattc ggcaacgagg
      241 tattgagtcg cgagatgctg ttgcggtatg tgcctatttt cgtagccatg ctcgtttggc
      301 tcacccgcat tctgttcatc ggcgcgttct ccgtggccgg cggtgatgtg ttcgagttga
      361 ctcgcagcca gccaggcaac aagcccggcg cgactccccg ccccgatctt ccggtcggcg
      421 cgcccgttcg ggcagcgtcc gctcagccgg caagttacca agccgccaca caaccggcag
      481 cacaagccag gaatcctcag caccaaacga ggcaatatcc atccgaacgg tcgaccatca
      541 cctacgagcc ggtggatgaa cccattgttg gccagccgac catgccccga acaaacggcg
      601 gcgcgccatc ccattcgacg tctcgtattc agcaacggcc accgatgccg ggcatgggac
      661 atagcccggc agccggtccg gttcaggctc gttcatccaa gccaggccat tgaaaaacga
      721 ctcattggcg gtaaactatg acaacttatc aggacaattc gtattattta attgactcac
      781 ccttgaatca atcgaacgat gacgaggttt ggcagtttcc tcccggctat ggtcagccga
      841 tgggaaaccc catggcgacc gttcagcgac gaatgatgtt aattctcgtc gcggcgacgt
      901 tgttcatcac ggcattcggc ttctcagtga ttcgagtgag caatatcggc acgaccacga
      961 acgactcgac aacgaccgat gaggcagccg cccagtcgca agcagcggca accgcatcag
     1021 cagaggagca acccatcgcg ccggtcgcgg caaccggcgg aattgcgccg ctcttcaccc
     1081 cggaaattca acattgggca ccgcagatcg tcgcctgggc caatgctgcg ggaatcgacc
     1141 cgaacatggc ggccaccgtc atgcagatcg aatcatgtgg cgatcccaac gccgtgtccg
     1201 gcgccggggc gcaggggctg tttcaagtga tgcccttcca tttcgatccg ggcgaggcga
     1261 tgcaagaccc cgacaccaac gcgcgacgag ggatggctta tctcgcgtta ggtatgcagc
     1321 aaaccggcgg cgacacaagc cgcacgttcg ccggatacaa cggcggccat ggaacggccg
     1381 cgcgcagttg ggacacctgg ccggctgaaa cgcaacggta ttacgtctgg gccaccggca
     1441 tttatggcga tgccgtcaac gggagcgcta ccagcgaaac gctgaatcaa tggctcaccg
     1501 cgggcggcgc cggcttgtgc caacaagcgg ccgcccggct ggggttgtga tctggccgga
     1561 ctccttcgcc ctcacaccat cccaaaggcg gtcggtcgca atgactgact gccttttttc
     1621 tttccacgtg gtcaatactc aacttgtcag gaagatcaag gaggtgaata atctttcaat
     1681 gctatttacg ggacatcgat atgcttgagt cggaacttta cgaggcgcga aactacgatc
     1741 gcggaatgtg gctgttggaa cgggcaaagc tctccgagct caggcagcgc gtcgttcagg
     1801 tggtgaccgg caatacgctg gaaatcggcg cgggaaccgg agccaatgtc gcccactatc
     1861 gtgacgacgt gacagtcaca gccattgacc tgcgaccaac ccacatcatg accgccggtg
     1921 acaaggctcg ccggcgccac ccaggctttg aattcacagc ggcctgcgcc gatgcacaat
     1981 ggcttccttt ttccgacaac acattcgaca cggtgttagg gactttggtc ttctgcagca
     2041 tcgccaggcc gcaggatgct ctacttgaaa ttcagcgcgt tctacgaccg ggcggccaac
     2101 tcatcctgct ggaacacgtg cgcggccgca cacctttcac gcgccgcctg accgattggc
     2161 ttcatcccct ctggttcgcc gcacagggcg tatgccatct taatcgggag actgcgacga
     2221 ctgtcgccga aacgggattc cgcatcgatt acacttctca acacgcccgt ggcctcattc
     2281 aggttatcca cgccacatcg ccaaaaacct gatatcaaac gaacaatagc gccaccccca
     2341 ccatggcgat cacggtgccg gcaatccctc ggcggctgac ccgatggcga tagatcagat
     2401 actcaagcgg gatgagcaaa accgg
//
LOCUS       DAOMQG010000468         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17364_length_2423_cov_8.581503, whole genome shotgun sequence.
ACCESSION   DAOMQG010000468 DAOMQG010000000
VERSION     DAOMQG010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17364_length_2423_cov_8.581503"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLC06_16060"
     CDS             <1..425
                     /locus_tag="PLC06_16060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRO92015.1"
                     /translation="RLGRDPEMRYMPDGTAVTSINVATDRRWTDKNTGQPVQETTWFR
                     VSVWGRQAESVNQYLSKGSMVLIEGHLRPDPQTGSPRTFTRQDGTTGTSFEMTAEAVR
                     FLSSRGDSGGSGSGGYDDSFQGGGGGKPAAHEEDDIPF"
     gene            complement(533..1357)
                     /gene="arsM"
                     /locus_tag="PLC06_16065"
     CDS             complement(533..1357)
                     /gene="arsM"
                     /locus_tag="PLC06_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRO92016.1"
                     /translation="MNENREQIHEAVRERYARLADGAAEGGCCSSGGDCCGSPADVAL
                     AEVTLEGEKLDRIYDDLYTADTKWLPKEVTGLSLGCGDPITLAGLQPGQTVLDLGSGG
                     GIDVFMAARQVGPTGYVIGVDMTPEMLAKAERNKAKVGLDNVEFRRGYIEDLPVDDDS
                     VDVILSNCVINLSPDKAAVFREAYRVLRSGGRLAVSDMMTLGRFTPEERANLPAWAGC
                     VTGAEDVALYAGVMRAAGFRDISIVDKGEPTVELAGHPLENGPTRLFSARVTAVKG"
     gene            complement(1411..1779)
                     /locus_tag="PLC06_16070"
     CDS             complement(1411..1779)
                     /locus_tag="PLC06_16070"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO92017.1"
                     /translation="MNIDMFEAPEESISFAKAIADETRQRVMGLLCCRWLCVGDIVEE
                     MQGVTQPTVSHHLAVLREAGLVHVRREGKQVFYSLNQDEVVDCCGRIMRIFAPERVDT
                     SATVDMASIEVREARAQVQR"
     gene            2010..>2423
                     /locus_tag="PLC06_16075"
     CDS             2010..>2423
                     /locus_tag="PLC06_16075"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92018.1"
                     /translation="MLPTTTQQRVAIIDLGSNTARLVVMAAVPGYAYRLEDEIREVVR
                     LRQGMTAAGLSDEAMARALVTLRLFKRFCDSTQVDLIIATATSAVRDAQNGAEFVRQV
                     ESGLGLPLRILDGEREAFYGVLGALNEVPLNDGYVL"
BASE COUNT          477 a          753 c          707 g          486 t
ORIGIN      
        1 ggcgtcttgg ccgggacccg gaaatgcgat atatgcccga tgggacggcc gtgacgagta
       61 taaatgtagc tacagatcgc cgctggacgg acaagaatac cggtcaaccc gttcaggaaa
      121 caacctggtt ccgcgtctct gtctggggcc gccaggctga gtcagtgaac cagtatctca
      181 gcaaaggcag catggtatta attgaggggc atttgcggcc tgatcctcag accggaagtc
      241 cgcgcacgtt tacgcggcaa gacgggacga cgggcacttc ttttgagatg acggccgaag
      301 ctgttcgctt tctgagcagt cgtggcgata gtggtggctc cggctccggc ggctatgacg
      361 acagcttcca gggcggcggt ggcggcaagc ccgcggccca cgaagaagat gacatcccgt
      421 tttaatggtt tgtcggtttg tagtttgcag atttataaat tagctctgta ggggcgggtt
      481 ttaaacccgc ccctacccat atttgtaacg gttaaatagc ctgatttatc ccctatccct
      541 tcaccgccgt cacccgcgcg ctgaacaatc gcgtcgggcc gttctccagc gggtgccccg
      601 ccaattcgac ggtcggttcg cctttatcga cgatcgaaat atccctgaag cccgccgctc
      661 gcatgacccc ggcatagagc gccacgtcct ccgcgccggt gacgcaaccg gcccaggccg
      721 gcaggttggc tcgctcctcc ggcgtgaatc ggccaagcgt catcatgtcc gacacggcca
      781 gccgcccgcc cgagcgcaac acccggtacg cctcacggaa gacggccgcc ttgtcggggc
      841 tgaggttaat aacgcagttg gacaggatga cgtcaaccga atcgtcatcc acaggcaggt
      901 cttcgatgta gccgcgccga aactccacgt tatccagccc caccttggcc ttgttgcgct
      961 ccgccttggc cagcatctcc ggcgtcatgt ccacgccgat gacgtacccg gtcggcccga
     1021 cctgccgcgc ggccatgaag acgtcgatgc cgccgcctga ccccagatcg agaaccgtct
     1081 ggccgggttg caggcctgcc aaggtgatgg ggtcgccgca accgagcgaa agaccggtta
     1141 cttctttggg cagccatttc gtgtcggccg tataaaggtc atcatagatc ctgtcgagtt
     1201 tctcaccctc cagcgtgacc tcggccagag cgacatcggc cggcgaacca cagcaatcgc
     1261 caccgctcga acaacatcca ccttcggccg ccccgtcagc caggcgcgca taccgttcac
     1321 gcaccgcttc gtgtatctgc tctctatttt cgttcacttt caatcacctc atattgatat
     1381 ccatctatgt gttattgtaa aaaaagaggc tcacctctga acctgcgcgc gcgcttcgcg
     1441 aacttcaatc gaggccatat cgacggtagc agatgtgtcc acccgctccg gcgcaaaaat
     1501 gcgcatgata cggccgcagc aatcgacgac ttcatcctga ttgagcgagt agaagacctg
     1561 cttgccctca cggcgcacgt ggaccagccc ggcctcgcgc aggacggcca ggtggtggga
     1621 gacggtcggc tgggtgacgc cctgcatctc ctcaacgata tcgcccacgc acagccagcg
     1681 gcagcacagc agacccatca cgcgttgtcg ggtctcgtcg gctatcgcct tggcaaagga
     1741 tatggattct tccggcgctt caaacatatc gatgttcatc aatatgggta ttatactggg
     1801 gatgggcggg atgtcaagag gaatgaccga cgaccgctga ccgacgagga agtacgaacg
     1861 acgaagtacg aagtacgaaa gagtcgcact cttgccttgg tccgtggttg atagacgatg
     1921 gacgacgcgc tcctgctgtc gtccgtggtc tgtcgtcggc ggtctatcac ctgtggcccg
     1981 tggttggccg tcggcgtata atgtcgatca tgctccctac taccactcaa cagcgtgtcg
     2041 ccatcattga cctgggttcc aacacggccc ggctggtggt tatggccgcc gtgccgggct
     2101 atgcctatcg cctcgaagac gagattcgcg aggtggtgcg tctgcggcaa ggcatgacgg
     2161 ccgccggatt gagcgacgaa gcaatggccc gcgctctggt gacattgcgc ctgttcaaac
     2221 gcttctgcga cagcacccag gtcgatctga tcatcgccac ggccaccagc gccgtccgtg
     2281 atgcccaaaa cggcgcggag ttcgtgcggc aggtggaatc aggcctgggg ctgcccctgc
     2341 gcatcctgga cggcgaaaga gaggcctttt acggcgtgct gggcgcgctg aacgaagtcc
     2401 cgctgaatga cggctacgtg ctc
//
LOCUS       DAOMQG010000469         2419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17398_length_2419_cov_8.115482, whole genome shotgun sequence.
ACCESSION   DAOMQG010000469 DAOMQG010000000
VERSION     DAOMQG010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17398_length_2419_cov_8.115482"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..854)
                     /locus_tag="PLC06_16080"
     CDS             complement(141..854)
                     /locus_tag="PLC06_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO92019.1"
                     /translation="MMILVVDDEAQIRKLLVTGLSGYGHEVITAANGEQALTLVAQRK
                     PDLVVLDINLGREPDGLETCRRLREWSRVPIIMLSVRNDETTKVQALDAGADDYLTKP
                     FGMEELRARIQAVVRRVVVEPGGAAESVIRTGELTIDLANRQVFVGGEEVHFTPQEYD
                     ILRLLATHAGKIMTHQVILRAVWGPEYADMTHYVRIYVNQIRKKLRENPAAGVRYILN
                     EPGIGYRFVNVGADERRDH"
     gene            complement(851..>2419)
                     /locus_tag="PLC06_16085"
     CDS             complement(851..>2419)
                     /locus_tag="PLC06_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRO92020.1"
                     /translation="PLATLPPQRYDPSVRHHPMTTKQTPPARPVLRQLGRFVPALLLV
                     ALTTLGLFFLRDQLSPATKSLIYMLVTLIAAVWLGTGPSVLAAVAGFFAINFFFVEPY
                     YTLDIATTRDLLSLLIYLAAALIAGRLAAYAQRQAAAAGLTAEQQAILYDLTSALNPL
                     IEPGRIQAELRQVLVRRMGAEQVDFLPHDPAWPHDPARRRDPAGNAVFVLLEAGDAVY
                     GTARVVFPATLSGSQHRLLLACAGQAALALQRVDLTGQAARSRTLAEADRLKTAILRA
                     VSHDLRTPITIIKTSAANLDELGDRLSAGDRRELAQSIEAQADELDRLVGNLLDLSRL
                     QAGAVVLHRELNSLEEIAGEVAARAYAAHHAERITLDFPDDLPLTHFDYGLMVQALGN
                     IVDNALRHEPAGTRVIIRGRVTGDEARVMVINHGPSIDPDEREKLMLPFHQSRADRAP
                     GEMGAANDGRSTHGGVGLGLAIARGILEAHHGDIRVEDTPGGGATFMLILPLDSRDER
                     RTTQAAAPPPEKLP"
BASE COUNT          400 a          768 c          827 g          424 t
ORIGIN      
        1 ctgatctact gaatactgat ctactgaata ctgatctact gaatactgat ctactgaata
       61 ctgatctact gaatactgat ctactgaata ctgatctact gaatactgat ctactgaata
      121 ctgatctact gatctactag tcagtggtcg cgtcgttcgt ccgcccccac attcacaaac
      181 cggtagccaa tccccggctc gttgaggatg tagcgcaccc ccgcggccgg gttttcgcgc
      241 agcttcttac gtatctgatt gacataaatc cgcacgtagt gggtcatatc ggcgtattcc
      301 ggcccccata ccgcccgcag gatcacctgg tgggtcatga tcttgcccgc gtgggtggcc
      361 agcagccgca agatgtcata ctcctggggg gtgaagtgga cttcctcgcc gccgacgaag
      421 acctgccggt tagccagatc gatcgtcagt tcgccggtgc ggatgacgct ttcggccgcg
      481 ccgcccggct ccacgacgac gcgccgcacg acggcctgga tgcgcgcccg cagctcttcc
      541 atgccgaacg gcttggtgag gtaatcatcg gcgcccgcgt ccagcgcctg aaccttggtc
      601 gtctcgtcgt tgcgcacgga gagcatgatg atgggcacgc ggctccattc gcgcaggcga
      661 cggcatgttt ccagcccgtc cggttcccga cccagattga tgtcgagcac cacgaggtcg
      721 ggcttgcgct gggccaccag cgtcagcgcc tgctcgccgt tggcggcggt gatgacctcg
      781 tggccgtaac cgctcagccc ggtgaccagc agcttgcgaa tttgcgcctc gtcatcgacg
      841 accagaatca tcatggcaat ttctccggtg ggggcgcggc ggcttgcgtc gttcgccgtt
      901 cgtcccggct atccagaggc aggatcagca taaaggtggc cccgccgccg ggcgtgtcct
      961 ccacgcgaat gtcgccgtgg tgggcctcca ggatgccgcg ggcgatggcc aaccccagcc
     1021 cgacgccgcc atgggtcgag cggccgtcgt tggcggcgcc catttcgccg ggcgcgcggt
     1081 cggcgcggga ttgatggaag ggcagcatca gcttttcgcg ttcgtcgggg tcaatcgacg
     1141 ggccatgatt gatgaccatg acgcgcgctt cgtcgccggt gacgcggccg cggatgatca
     1201 cgcgcgtgcc ggccggttcg tggcgcaggg cgttatcgac gatgttgccc agcgcctgca
     1261 ccatcaggcc ataatcgaag tgggtcagcg gcaggtcgtc gggaaagtcg agggtgatgc
     1321 gctcggcgtg gtgggcggcg taggcccgcg ccgccacctc tccggcgatc tcctccagcg
     1381 agtttagctc gcgatggagc accaccgccc cggcctgcag gcgcgacagg tcgagcagat
     1441 tgcccaccag ccggtcaagc tcgtcggcct gggcttcgat ggattgggcc agctcgcgcc
     1501 ggtcgccggc cgaaagccgg tcgcccagct cgtcgagatt ggcggccgag gtcttgatga
     1561 tggtgatcgg cgtgcgcagg tcgtgggaga cggcgcgcag gatggccgtc ttcaaccggt
     1621 cggcttcggc caatgtgcgg ctgcgcgcgg cctgaccggt cagatcgacc cgctgcaggg
     1681 ccagcgcagc ctgaccggcg caggccagca gcagccggtg ttgcgagccg ctcagcgtcg
     1741 ccgggaagac cacccgcgcc gtgccataaa cggcgtcgcc ggcctccagc agcacgaaga
     1801 ccgcgttccc cgccgggtcg cgccgccgcg cgggatcgtg gggccatgcg ggatcgtggg
     1861 gcagaaagtc gacctgctcc gcgcccatgc gccgcaccag aacctgccgc agctccgcct
     1921 ggatgcgccc cggctcgatg agcgggttca gggcgctggt cagatcgtag aggatcgcct
     1981 gttgttcggc cgtcagcccg gcggcggcgg cctgtcgttg ggcataggcg gccagtcgcc
     2041 cggcgatgag cgccgccgcc agatagatga gcaggctcag caggtcgcgc gtcgtggcga
     2101 tatcgagggt gtaatagggt tcgacgaaga agaaattgat ggcgaagaac ccggccacgg
     2161 cggccagcac cgatggcccc gtgcccagcc acacggcggc gatcaaggtc accagcatgt
     2221 agatcagcga tttggtagcg ggcgatagct ggtcgcgcag gaagaacagg cccagcgtgg
     2281 tgagggccac cagcagcagc gccggcacaa agcggccgag ctgccgcagg acgggccggg
     2341 cgggtggggt ctgcttcgtc gtcatgggat gatgccgcac ggacggatca tagcgttgtg
     2401 gggggagggt ggcaagggg
//
LOCUS       DAOMQG010000470         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17436_length_2415_cov_6.812288, whole genome shotgun sequence.
ACCESSION   DAOMQG010000470 DAOMQG010000000
VERSION     DAOMQG010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17436_length_2415_cov_6.812288"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1039)
                     /locus_tag="PLC06_16090"
     CDS             complement(17..1039)
                     /locus_tag="PLC06_16090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO92021.1"
                     /translation="MSTEIVPFHVGMLPAAAELLSRRHARDRAMMPLLPARFEQPRMA
                     QAAVREVWSNPHTSGVAAWRDGRLVGYLLGQAKFETHRGRHVWIPLPGYALGEGEPAD
                     LYAELYAAAGPEWLRLGAFDHYLMMPAEDRDGLNAWFSLSFGKEQAHAILSLTGPLPE
                     RPAVPGVVIRRAGEADRDAFVGDMSPILRRHMAGPPVWAASLPERVEPMREGFVEMLT
                     DESARVWIAEAEGNGDDSGRMLGYVLLYPASPSDDNLTVSVSERTVLLEVAAIRPEAR
                     GRGIGQALAVAGLADAAALGYRVCITDWRTPNIEANRFWPKLGFRPAVYRLARKVDPR
                     IMWATF"
     gene            complement(1089..1421)
                     /locus_tag="PLC06_16095"
     CDS             complement(1089..1421)
                     /locus_tag="PLC06_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HRO92022.1"
                     /translation="MNDAATVHEAVRQVKAGELKRPTNCVHVDALQEVDPRTAGCEDC
                     LKAGSTWIHLRVCLTCGYVGCCNSSPGKHSSKHAHDTGHPVVMSMETGEDWMWCYQDE
                     DYIAPRRK"
     gene            complement(1558..>2415)
                     /locus_tag="PLC06_16100"
     CDS             complement(1558..>2415)
                     /locus_tag="PLC06_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017543668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92023.1"
                     /translation="GPHKSRTWSPVLISEADGWISQYRGLWGLDTRDRMGGERAPAGP
                     MYERSGAIRKSWYDPLGWAGLDKVFPPSDLPEEIDERIADVEAGLESLDERITLDRER
                     LRVLALDLESLRAAEHFSGIYQMKAKELDAAQEGFQALVQRQVELIETRNALLHYRRR
                     ILAGDPGPPAAHLQHAHRPTPPVEHHRALEIWSAISGAMALLSFGYLIIYRPSYWFLM
                     VLIVGVLFGFIDSITRGSTDRYLVNLAVALATVNALILFIEFWQIALILPLILLVIFV
                     LRDNLRELR"
BASE COUNT          430 a          806 c          798 g          381 t
ORIGIN      
        1 attcgtaatt cgtaattcaa aacgtggccc acataatgcg cgggtcgacc ttgcgggcca
       61 ggcgatagac ggcggggcgg aagccaagct tcggccaaaa gcggttggcc tcgatgttgg
      121 gcgttcgcca atcggtaatg cacacgcgat agccgagcgc ggcggcgtcg gccagaccgg
      181 cgaccgccag cgcctgccca atgccgcggc cgcgcgcttc cggccgaata gcggccactt
      241 ccagcagcac cgtccgctcg gagacggaga cggtcagatt gtcatccgac ggcgacgccg
      301 ggtacagcaa tacatagccc agcatgcggc cggagtcgtc gccgttgcct tcggcttcgg
      361 cgatccacac ccgcgccgac tcatcggtca gcatctcgac gaacccctcg cgcatcggct
      421 cgacccgctc gggcagcgac gcggcccaca ccggcggccc agccatgtgc cggcgcagga
      481 tgggcgacat gtcgccgacg aacgcgtcgc gatcggcttc gccagcgcgc cgaatgacta
      541 cgccgggcac ggccggccgc tccggcagcg gcccggtcag cgacaggata gcgtgggcct
      601 gctccttgcc gaagctcagc gagaaccagg cgttcagccc gtcgcggtct tcggcgggca
      661 tcatcagata atggtcaaac gcgcccaggc gcagccattc cggcccggcg gcggcgtaga
      721 gttcggcata gagatcggcc ggttcgccct cgcccagcgc gtagcccggc aggggaatcc
      781 acacatggcg gccgcgatgg gtctcgaact tcgcctggcc cagcagatag ccgacgaggc
      841 ggccgtcgcg ccaggcggcc acgcccgacg tgtgggggtt gctccagact tcgcgcacgg
      901 cggcctgggc catgcgcggc tgctcgaagc gcgccggcaa cagcggcatc atggcccgat
      961 cgcgggcatg tcgccgcgag agcagctcgg ccgccgccgg aagcatgccg acatggaagg
     1021 ggacgatctc ggtactcata gtagttgccc cgcgcccttg cgcggtcgaa aatgtcaccg
     1081 gcccgcgctc atttgcggcg cggggcgata tagtcctcgt cctggtagca ccacatccag
     1141 tcctcaccgg tctccatgga catgacgacg gggtggccgg tgtcgtgggc gtgtttggag
     1201 gaatgcttgc cgggcgagct gttgcagcag ccgacgtaac cgcaggtcag gcagacccgc
     1261 agatggatcc acgtgctgcc cgccttcagg caatcttcgc agcccgccgt gcgcggatcg
     1321 acctcctgga gcgcgtccac atggacgcag ttggtgggtc gtttgagttc gccggctttc
     1381 acctggcgaa cggcttcgtg gacagttgcg gcatcgttca ttcgacctct cctgtagagt
     1441 gcgatcaggg tgtgaccata tccccgccgg tgcagcccga gcgtggagtt tgagtgtacg
     1501 tctatcatgg gggactgtca aacgagggat agggcataga aaccgggttt ccaaacccta
     1561 cctcagctcg cgcaaattat cacgcaagac gaagatgacc agcaggatca gcggcaggat
     1621 gagcgcgatc tgccagaact cgatgaacag gatcagcgcg ttgacggtcg ccagggccac
     1681 ggccaggttc accagatagc gatcggtgct gccgcgggtg atcgagtcga tgaagccgaa
     1741 caggacgccc acgatgagga ccatcaaaaa ccagtagctg gggcgataga tgatcagata
     1801 gccgaaggac agcaaggcca tcgcgccgct gatcgccgac catatctcca gcgcgcggtg
     1861 gtgctcgacc ggcggcgtgg ggcgatgggc gtgctgaaga tgcgccgcgg gcggccccgg
     1921 atcaccggcc aatatgcgcc gtcgatagtg cagcagcgcg ttgcgcgtct cgatcagctc
     1981 cacctgacgc tggacgagcg cctggaatcc ctcttgcgcg gcgtcgagtt ccttggcctt
     2041 catctgatag ataccgctga aatgctcagc ggcgcgcagc gattcgagat cgagcgccag
     2101 cacccgcagc cgctcgcggt cgagtgtgat gcgctcatcc agcgactcca gcccggcctc
     2161 cacgtcggcg atgcgctcgt cgatctcctc cggcaggtcc gacggcggaa acaccttgtc
     2221 gagtcccgcc cagcccagcg ggtcatacca cgacttccgg atggccccgg agcgctcata
     2281 catcggcccg gccggggcgc gctcgccgcc catccggtcg cgcgtgtcca gtccccacag
     2341 gccgcgatac tggctgatcc agccgtcggc ctcggaaatg agcaccggcg accacgtgcg
     2401 cgacttgtgc gggcc
//
LOCUS       DAOMQG010000471         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17472_length_2410_cov_5.653503, whole genome shotgun sequence.
ACCESSION   DAOMQG010000471 DAOMQG010000000
VERSION     DAOMQG010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17472_length_2410_cov_5.653503"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLC06_16105"
     CDS             <1..734
                     /locus_tag="PLC06_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRO92024.1"
                     /translation="RRVTPTTYRPGETIIRQGETGDRFYILTEGRAEVFVDHPDGHII
                     LVDRLGPGQYFGEMALLGQRARRATVRVAEGAPAAVVALDPATFNRLVEESPALRDEL
                     QRIVSLRQVQSQVEALATVDREQLRELMKDAPTLTFQPGETILQQGALGDVFYFLLDG
                     EVEVSTRQGNHAERLIDRLGPGRYFGEMALLGNKRRSATVRAAGDGPVRVLELDAAAF
                     EQLRQLSGQFADAVRETAAERQTRV"
     gene            828..>2410
                     /locus_tag="PLC06_16110"
     CDS             828..>2410
                     /locus_tag="PLC06_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRO92025.1"
                     /translation="MNTLWRKTLRDLWLYKARTVLVVLAIAVGTASAGVATTSLIVLR
                     RDLRAGFLGTNPAHAVLDVAGAAPGAGAIPIDEALAEQVAALPEVGDAVARRMTPARL
                     ALPGGEERFLQLWTLPDFDSPVAALYPQAGAVVPPPAGSLLLERSAAPVLGIAIGDTV
                     TVQPIDGGATELQVAGFVNDLSVAPTTVQPGVYGYMTDATAAQLGLAEGYNQLHLTVA
                     APSPDRAAVETAVTAVTEWLKDEGVVVTRAAIPEPNVHIMQGSVDTGLLMIGILGVLT
                     LVLSAFLVTNVMSAVVAQQVPIIGVLKALGSERGLVLRQYGRMVALFGLMALALAVPL
                     GLMGAWFMSSTLAGQLNYDIPSFGLPWQTVVVQLVGALAIPALAALGPLRAAANLTIR
                     EAFSQTGDTQYEARGENASPLNSYFVSRISYLVAWRNVARRKLRLGLTLVGLALAGAI
                     FIATFGLKLGLDEAIEVLVGEFSADVVIDFAQPEEAQRMEQEAAPVAATMPVIERVET
                     WGVADARRVFPDGRVGSSFT"
BASE COUNT          378 a          737 c          880 g          415 t
ORIGIN      
        1 agcggcgcgt gaccccgacg acctatcgcc ccggcgagac catcatccgc cagggggaga
       61 cgggcgaccg gttctacatc ctgaccgaag gccgggccga ggtgtttgtc gatcatcccg
      121 acggccacat tatattggta gaccggctgg ggccggggca gtacttcggc gagatggccc
      181 tgctggggca gcgggcgcgc cgggccacgg tgcgcgtggc cgagggcgcg ccggcggcgg
      241 tagtcgccct cgatccggcc acgttcaacc ggctcgtgga ggaatccccg gcgctgcgcg
      301 acgagttgca gcggattgtc agcctgcgac aggtgcaaag ccaggtcgag gcgctggcga
      361 cggtggatcg cgagcaactg cgggaactga tgaaagatgc ccccacgctg acctttcagc
      421 cgggagagac gatcttgcag caaggcgcgc tgggcgatgt gttctatttt ctgctggacg
      481 gcgaggtgga agtatccaca cggcagggaa accacgccga gaggctcatc gaccggctgg
      541 ggccggggcg atacttcggc gagatggcgc tgctgggcaa caagcggcgc tcggccacgg
      601 tgcgggcggc gggcgatggg cctgtgcggg tgctggagct tgacgcggcg gcattcgagc
      661 agttgcgaca actgtcgggc caattcgccg acgccgtgcg cgagacggcc gccgagcggc
      721 aaacgcgggt atgatccaga cctgacaggt cggcgaccac gacacctgtc taaccaaaac
      781 ttttatcgcc gacctgtcag gtctatgatc cggcgacacg gatatttatg aacacactgt
      841 ggcgaaagac attgcgggat ttgtggctct ataaggcgcg gacggtgctg gtggtgctgg
      901 ccatcgccgt gggcacggct tcggccggtg tggccacaac cagcctcatc gtcctgcgcc
      961 gcgatttgcg cgccggcttt ctgggcacca acccggctca tgccgtgctg gacgtggccg
     1021 gggcagcccc cggagccggg gcaatcccca tcgatgaggc attggccgaa caggtggccg
     1081 cgctgccgga agtgggcgat gccgtggcgc gacgcatgac gcccgcgcgg ctggcgctgc
     1141 ccggcggcga agagcgcttc ctgcaactgt ggacgctgcc tgacttcgat tcgcccgtag
     1201 ccgccctcta tccgcaggcg ggggccgtcg tgccgccgcc ggccggttcg ctgctgctgg
     1261 agcgctcggc cgctccggtg ctgggcatcg ctatcggcga cacagtgacc gtccagccaa
     1321 tcgacggcgg cgcgaccgag ctacaggtgg ccgggttcgt caacgatctg tccgtcgctc
     1381 ccacgacggt gcagccgggc gtctacggct acatgaccga cgccacggcg gcgcaactgg
     1441 ggctggccga aggctataac cagctccatc tcaccgtcgc cgcgccgtcg cccgaccgcg
     1501 cggccgtgga aacggccgtg acggccgtca cggagtggct gaaggacgaa ggcgttgtcg
     1561 tgactcgcgc ggcgatccct gaacccaacg tccacatcat gcagggcagc gtcgacaccg
     1621 gtctgctgat gatcggcatt ctgggcgtgc tgacgctggt gctgagcgcg tttctggtga
     1681 cgaatgtgat gtcggccgtc gtggcccagc aggtgccgat catcggcgtg ctgaaggcgc
     1741 tgggcagcga gcgcggattg gtgttgcggc aatatggccg gatggtggcg ctatttggtc
     1801 tcatggcgct ggcgctggcc gtgccgcttg gcttgatggg agcgtggttc atgtccagca
     1861 cgctggccgg gcagctcaat tacgatatcc cgtcattcgg cctgccgtgg cagacggtgg
     1921 tggtgcaact ggtgggggcg ctggcgattc cggcgctggc cgcgctgggg ccactgcggg
     1981 cggccgccaa cctgaccatc cgcgaagcat tcagtcaaac tggagatacg caatacgagg
     2041 cacgaggcga gaatgcgtct cctctcaact cgtatttcgt atctcgtatc tcgtatcttg
     2101 ttgcgtggcg caatgtggcc cggcgcaagc tgcggttggg gttgacgctg gtggggctgg
     2161 ccttggcggg ggcgatcttc attgcgacct tcggcctgaa actggggctg gatgaggcga
     2221 tcgaggtgct ggtgggcgag ttctcggccg acgtggtgat cgatttcgcg cagccggaag
     2281 aggcgcaacg gatggagcaa gaagcggcgc ccgtggcggc gacgatgccg gtcatcgagc
     2341 gcgtcgagac ctggggcgtg gccgacgcgc gccgcgtctt ccccgacggc cgcgtgggca
     2401 gcagcttcac
//
LOCUS       DAOMQG010000472         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17571_length_2397_cov_7.346285, whole genome shotgun sequence.
ACCESSION   DAOMQG010000472 DAOMQG010000000
VERSION     DAOMQG010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17571_length_2397_cov_7.346285"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLC06_16115"
     CDS             <1..215
                     /locus_tag="PLC06_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92026.1"
                     /translation="VNDSGLYTLPYRLSRRKEKFVEIRFSRPFLPDMYWESVLAGKLH
                     RRNNAHKQKRAGSSCRRVLYYLSISA"
     gene            complement(311..571)
                     /locus_tag="PLC06_16120"
     CDS             complement(311..571)
                     /locus_tag="PLC06_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92027.1"
                     /translation="MTDKLPSRPTLDIREALNELLQRNDRTQDKVVGDMFLGSDIFIA
                     RLRRPKVLEGDWEDMDRTVDLFDLIDAHQKEAPPRSRRDSKE"
     gene            779..2158
                     /locus_tag="PLC06_16125"
     CDS             779..2158
                     /locus_tag="PLC06_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRO92028.1"
                     /translation="MSHPGKQEIESRIDERINETLEYWIEQLGRLCAQPSISAQKLGI
                     NECADLTATMLREEGFSAEILPTGGSPIVFGDREGSSERTLLFYNHYDVQPPEPLELW
                     DTPPFTLTRVGDSLFARGVSDDKGQLITRMAATAAVRDVMGELPLRIKYFVEGEEEVG
                     SPNIPGAIDAYKEKFAADGCIWEFGSVDYSGRPEQTLGMRGLCYIELEVKTASLDCHS
                     GLGGSLIPNAAWRLVWALSTIKGPDERIRIPGFYDNALSATERDLAYLAQQPDHSASM
                     LETYGVKEFLKGMTGGTELRRAAVFEPTATINGLNSGYQGPGAKTVLPAAASAKIDFR
                     LVPDQTPEEIVPLLRAHLDAQGFPDVEVRYLAGVKPARTDPDNPFVQMSIQAAHDVYG
                     LTPIVEPLIGGSGPLHPFIHELGLPVVTSGCGHPKSRVHAPNENIRIPDFVNGIRHTA
                     RIITRFADL"
     gene            complement(2237..>2397)
                     /locus_tag="PLC06_16130"
     CDS             complement(2237..>2397)
                     /locus_tag="PLC06_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRO92029.1"
                     /translation="SDGIKYYLADGSWLLIRPSGTEPVLRVYAEAPNDERVRALLDFG
                     EAIAAAEK"
BASE COUNT          548 a          718 c          620 g          511 t
ORIGIN      
        1 gggtcaacga ttcaggatta tatactctgc cctatcggtt gtcaagacga aaagagaaat
       61 tcgttgaaat tcgtttttcg aggccttttc tccctgatat gtattgggaa tcagttctcg
      121 caggcaagct gcaccgccga aataatgccc ataaacaaaa acgcgccggt tcgtcatgcc
      181 ggcgcgtttt atattacctg tctatttcag cttgaagatc tacgaggagt tacgcgaggt
      241 tattcttata tggagtgtcg aattgctaat cgcttcccgc aacgtagagg actaattgca
      301 tctgccggcg ctattccttg ctgtcccggc gcgatcgagg aggggcttcc ttttgatggg
      361 catcaatcaa atcaaataga tcaaccgttc ggtccatatc ttcccaatcc ccctccagaa
      421 ccttcggccg acgcagccgc gcgatgaaga tatcggaacc cagaaacata tcgccgacca
      481 ccttgtcttg agtgcgatca ttacgctgaa gaagctcgtt caacgcctcg cgaatatcca
      541 gagtcggccg ggacggcaac ttgtcggtca tctagttcct atacataacg atgtaaccat
      601 gatcgtccac attgtaaccc gcacccccat gctcgcacgt aaaaagcacg ttgaacttag
      661 ccgaaacgcc atcgctgtgc cgggtggagc aatccttacg ccagtcgccg tccccggtta
      721 gttgcgatat agttcgagaa attccactcc actaaatacc tgaagagagg ttagagcaat
      781 gagccatcca ggcaaacagg aaatcgaatc ccgaatcgat gaacgaatca atgagaccct
      841 tgaatactgg atcgagcagc tgggccgtct ctgcgcccag cccagtatct ccgcccaaaa
      901 actgggcatt aatgaatgcg ccgatctgac ggcaaccatg ctgcgtgaag aaggcttctc
      961 ggccgaaatt ctgcccaccg gtggcagtcc tatcgtattc ggcgatcgcg aggggagcag
     1021 cgagcgaaca ctcctgttct ataatcatta cgatgtccaa ccgccggagc cgttggagct
     1081 atgggacacg cctcccttca ctctgactcg cgttggtgac agcctgtttg cgcgcggcgt
     1141 cagcgacgac aaggggcaac tcatcacacg gatggccgcc acggccgccg ttcgcgatgt
     1201 gatgggcgaa cttccccttc gaatcaagta ctttgtcgag ggcgaggaag aggtcggcag
     1261 ccccaacatt ccgggcgcaa tcgatgccta caaagagaaa ttcgcggccg atggctgcat
     1321 ttgggagttc gggtccgtcg attattccgg ccggccggaa caaacattgg gaatgcgcgg
     1381 cttgtgctac atcgaattgg aagtcaagac ggcctcgctc gattgtcact ccgggctggg
     1441 tggctcgctg atccccaacg cagcctggcg acttgtttgg gcgctcagca ccatcaaagg
     1501 gccggatgaa cgcattcgca tccctggctt ctatgacaac gcattgtccg ctaccgagcg
     1561 cgatctcgcc tatctggcgc aacaacccga ccactcagcc agtatgctgg aaacttacgg
     1621 agtgaaagag ttcctgaaag ggatgaccgg cggcacggaa ctcagacggg cggccgtctt
     1681 tgagccgacg gcgaccatca acggattgaa ttcaggctac caggggccgg gagccaaaac
     1741 cgtgctgccg gccgcggcca gcgccaagat cgatttccgc cttgtgccgg accaaacgcc
     1801 ggaagagatc gtgcctctgc tgcgcgccca ccttgacgca caggggttcc ccgatgtgga
     1861 agttcgttac ctggcggggg taaaaccggc acgaaccgac cccgacaacc cgttcgtgca
     1921 gatgtcgatc caggccgctc atgacgtcta tgggcttacc cctatcgtcg agcctctgat
     1981 cggcggaagt ggccccctcc atccattcat tcatgagctt ggcttgccgg ttgtgacctc
     2041 gggttgcggt catccaaaaa gccgggtcca cgcccccaac gaaaacattc gcatccccga
     2101 cttcgtgaac ggcatccgcc atacggcccg catcatcacc cgattcgctg acctctaacc
     2161 ctaaaaaggc tctaaacaaa tagagcgtga tgccattatc ggctatcacg ctctatttag
     2221 atcacattgg ccggttctac ttttcggctg ctgcgatagc ctcgccgaag tcgagcaatg
     2281 cccgcaccct ttcgtcgttc ggtgcttccg cgtacacccg caacaccggc tccgtacccg
     2341 atggccggat caacaaccag ctaccatccg ccagataata cttgatccca tcgctgg
//
LOCUS       DAOMQG010000473         2383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17686_length_2383_cov_9.628436, whole genome shotgun sequence.
ACCESSION   DAOMQG010000473 DAOMQG010000000
VERSION     DAOMQG010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17686_length_2383_cov_9.628436"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..940
                     /locus_tag="PLC06_16135"
     CDS             179..940
                     /locus_tag="PLC06_16135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRO92030.1"
                     /translation="MLDFTDKVVLVTGSSRGIGRSIAQLFAERGARVAVHYHTNRAAA
                     VVALASLAGEGHILVQGDIADPAAVAAIVDDVVAQAGRIDILVNNAAIYEDHPLAETD
                     YQTWQTSWRRTIETNVLGTANMSYCAARHMIAQGGGRIVNVSSRGAFRGEPTAPAYGA
                     SKAALNAMSQSLAKYLAPYNISVAVAAPGFVETDMARGALAGESGNEIRGQSPFNRVA
                     KPEEVAYAVLFLASEGAEFMTGAIIDINGASYLRT"
     gene            complement(968..1858)
                     /locus_tag="PLC06_16140"
     CDS             complement(968..1858)
                     /locus_tag="PLC06_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRO92031.1"
                     /translation="MTSESDVKPIAFGMSTAALFAVLLVVDSLHFVFARALLPHLPPE
                     MSSFLVLAVAAVQVGLYQAVRGRIHMDVLWSHWRFFAAIGILVAASTVINYAAVSFID
                     PGTASLLGKTSVLFSLLLGLFWLRERLKPIEVLGALLALIGAFIIGFQPGDYLRFGSL
                     MVLISTFLYALHTAIVKRHGERIDFGDFFLFRIGSIAFFLFLFAVARGQLVWPTPIAW
                     VLVVVAGTVDVVISRSLFYLSLRRMRMSIHTVILTLSPVVTIVWALILFGDMPTRISL
                     IGGTFVILGVFAVTTRTRRS"
     gene            complement(1876..2346)
                     /locus_tag="PLC06_16145"
     CDS             complement(1876..2346)
                     /locus_tag="PLC06_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRO92032.1"
                     /translation="MRNQPHIFQINLSQGGVPKMPQPSAEVTSLGLSGDLHNDTVHHG
                     GPERAISFYSLEIIRALQDEGHPIFPGSTGENLTISGLDWTIVDAGLRLALGDTVLIE
                     VTRYVTPCKTIRDSFVDRQFDRISWRTNPGWARAYARVLIPGEIAVGDSVKIVD"
BASE COUNT          577 a          677 c          701 g          428 t
ORIGIN      
        1 gttggtttga tacgctgatc gcgtcggtgc ataccgtcct ctcaacgtac cggaggatag
       61 cagtctttag actgtgtagc aaccccatgg caaggcaaat cgcattgtgg cgtgttcatc
      121 ttgcaaggcc tatcaggttc gatgataatg cctgctatca cacccacagg agatcgccat
      181 gctggatttc acagacaagg tagttttggt aaccggctca tcgcgcggca ttggccgctc
      241 catcgctcag ctattcgcgg agcgcggcgc gcgcgttgcc gtccattacc ataccaaccg
      301 cgcggcggcc gtcgttgcct tggcctcgct cgccggagaa ggccacatcc tggttcaggg
      361 cgacatcgct gatccggcgg ccgtcgcggc tatcgtcgat gacgtggtgg cgcaggccgg
      421 aagaatcgac attctggtca acaacgcggc gatctatgag gatcatccct tggctgagac
      481 cgattaccag acatggcaga caagctggcg gcggacaata gaaacgaacg tcctgggcac
      541 ggcgaacatg agctattgcg cagctcgcca tatgattgcc cagggtggcg gccgcatcgt
      601 gaatgtctcc tcacgcggcg catttcgcgg cgagccaacc gctccggcct acggggccag
      661 caaggccgcg ctcaacgcaa tgagccagtc gttggccaaa tatctggctc cgtacaacat
      721 ctcagtggct gtagccgcgc cgggattcgt cgaaacagac atggcgcgtg gcgctcttgc
      781 cggggagagc ggcaatgaaa ttcgcggtca aagcccattc aaccgcgtgg ccaagccgga
      841 agaggtcgcc tacgccgtcc tgttcctggc ctcagaggga gccgaattta tgaccggggc
      901 gattatcgac atcaatgggg catcatatct gaggacatga ggttcattca atagcatcct
      961 gatgttgtca ggaacggcgg gtccgcgtcg tcacggcgaa cacgcccaaa atgacgaaag
     1021 ttccgccgat gaggctgatg cgggtgggca tgtcgccaaa caggattaat gcccaaacaa
     1081 tcgtcacaac cggactgagg gtgaggataa cggtgtggat gctcattcgc atccgacgca
     1141 gtgatagata gaaaagcgat cgactgatca ccacgtccac cgtcccggcc acgacgacga
     1201 gcacccaggc gatcggtgtc ggccacacca gttgaccgcg cgcgaccgcg aacaggaaga
     1261 ggaagaatgc gattgagcct atgcggaaga ggaagaaatc accaaagtcg atccgctctc
     1321 cgtgtcgctt cacaatggcc gtatgcaacg catacaaaaa agtggagatg agaaccatca
     1381 gggagccgaa gcggaggtag tcgccaggct ggaagccgat gatgaacgcc ccaatcagag
     1441 ccagaagcgc gcccaaaacc tcgataggct tcaagcgctc gcgaagccag aatagcccca
     1501 ggaggaggga aaacaggacg gaggtcttgc ccaacaagga ggccgtcccc ggatcgatga
     1561 aggaaactgc agcataatta atcaccgtcg acgcggcgac gagaatgcct atcgcggcaa
     1621 aaaatcgcca gtgggaccac aagacgtcca tatgaatccg accgcgaacc gcctgatata
     1681 gcccaacttg caccgccgcg accgccagaa caaggaacga cgacatttcg ggcggcagat
     1741 ggggcaagag cgcccgggca aagacaaagt gcaggctatc cacaaccagc agcacggcaa
     1801 agagagcggc cgtgctcatg ccgaacgcga tcggtttgac gtcggattca gatgtcattt
     1861 tgcgggaaaa atcggtcagt ccacgatctt cacactgtcg cccacggcga tctctcccgg
     1921 aatcagaaca cgggcatagg cgcgcgccca tccgggattg gtgcgccagg agatccgatc
     1981 gaattgccgg tcaacaaatg aatcgcggat ggttttgcat ggcgtgacgt agcgagtgac
     2041 ctcaatcagc accgtgtccc ccagagccag ccgcaagccg gcatcaacta tcgtccagtc
     2101 caaccctgat atcgtcaggt tttcgccggt ggagccggga aaaatcgggt gaccttcgtc
     2161 ctgcaacgct cgaataatct ctagcgagta aaagcttatc gctctctcgg ggccaccgtg
     2221 atgcactgtg tcattgtgaa ggtcaccgct caacccgaga gaggtcacct cggccgaagg
     2281 ttgcggcatt ttagggacgc cgccctgtga aagattgatc tgaaaaatat gaggttgatt
     2341 acgcattagt caaaatcctg tggctgggga gtgtagccgc tcg
//
LOCUS       DAOMQG010000474         2381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_17703_length_2381_cov_9.575236, whole genome shotgun sequence.
ACCESSION   DAOMQG010000474 DAOMQG010000000
VERSION     DAOMQG010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17703_length_2381_cov_9.575236"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1779
                     /locus_tag="PLC06_16150"
     CDS             130..1779
                     /locus_tag="PLC06_16150"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRO92033.1"
                     /translation="MTKFIFFTGGVMSSVGKGVTAAALGRLLKARGFSVTAQKLDPYF
                     NVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFIDINLNQAANVTSGRVALEVTARE
                     RRGDYLGSTVQVIPHITNEIKRSIFQVAEKSQAEIVLVEVGGTVGDIESEWFMEALRQ
                     MRSDVGRDNTLYVHVTYLPFSSASRELKTKPTQHSVRDLRRIGIQPDVIIARADHPIP
                     EEHLNKISLFCDVSREAVIPAVTSDILYNIPLLLEETQMAEVVMERLGLVAKTEPDLA
                     EWEAMIGEIRREKPAIRIALVGKYVELHDAYMSVRESLYHAGVANDRQVEIEWIYSGD
                     LEKGKGWEQLDGVSGVVVPGGFGERGIEGKIQVARWARENQVPYLGLCLGMQVMCIEF
                     ARHILGSDEPNSTEFDRNCEHPIISLMPDQHELADMGGTMRLGAYPAILKHGTKAYAA
                     YGEERISERHRHRWEFNNAYRELLSAAGMVFSGLSPNGRLVEISEMRDHPFMLGTQFH
                     PEFKSRPNRPHPLFNAFMAAAVDYQVAAQKQEQNSDETNSG"
     gene            complement(1818..>2381)
                     /locus_tag="PLC06_16155"
     CDS             complement(1818..>2381)
                     /locus_tag="PLC06_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRO92034.1"
                     /translation="DLDSPLIPTPTETAEAIGSGFTDKAATGLIMIGLLVIIALALAR
                     TYRQATFAARDSEHSRAIAESDEKPGIGRRLLERLGVLRHWRAAASVRRIYRQMCAAA
                     TAAGYPRSGSETPYEYLSSLSRVWPEYTTETRLITEAFIRVRYGEVPETAEELESLRS
                     AWRQLEAEEPKRREKTGEQGPILTKRE"
BASE COUNT          498 a          637 c          722 g          524 t
ORIGIN      
        1 ctccggtacg ttgagaggac ggtatgcacc gacgcgatca gcgtatcaaa ccaacgacag
       61 attatttgtt tagaggtggg gcatgtcgcc caggatgaca tgccccacct ctctttaatg
      121 ggagtggcga tgacaaagtt tatctttttt accggcggcg ttatgagttc cgtcggcaaa
      181 ggcgtgacgg cagcggcgtt gggacgtttg ctcaaggcgc gaggctttag cgtgaccgcg
      241 caaaagcttg atccctactt caatgttgat ccgggaacga tgtcacctta tcagcacggc
      301 gaagtgttcg tcaccgaaga cggggcggag actgacctgg atctgggtca ctacgagcgt
      361 tttatcgata tcaatctcaa tcaggcagcg aatgtcactt ccggccgcgt tgctctggaa
      421 gtcaccgccc gcgagcgccg aggtgattat ttgggcagca ctgttcaggt catcccccac
      481 atcactaacg agataaaacg cagcatcttt caggtggccg agaaaagtca ggcggagatc
      541 gtcctggtcg aagtgggtgg cacggttggt gatattgaga gcgagtggtt catggaggcg
      601 ctgcgccaga tgcggtccga cgtcggccga gacaatacgc tctatgtcca cgttacctac
      661 ttgcctttct cgtctgccag ccgcgagctt aagaccaaac cgacccagca cagtgtcaga
      721 gatttgcgcc ggatcggtat tcagcctgat gttatcattg cccgcgccga tcatcccatc
      781 cctgaagaac acttgaacaa aatttctctc ttttgcgatg tgtctcggga ggcggtcatt
      841 cccgcagtga caagcgatat tctctacaat atcccgcttc tgctggagga gactcaaatg
      901 gctgaggtcg taatggagcg cctgggtctg gttgcaaaga cagagccgga cctggccgag
      961 tgggaagcga tgattggtga aattcgacgc gagaagccgg cgatacgaat cgccctcgtg
     1021 ggcaagtatg tcgagctgca tgacgcgtat atgagcgtcc gcgaatcgct ttatcacgcc
     1081 ggggttgcca atgatcgcca ggtagagatc gaatggattt attccggcga tttggaaaag
     1141 ggcaaaggct gggaacagct cgatggcgtt agcggggtcg tggttccggg tgggttcggc
     1201 gagcgcggca tcgaaggaaa aattcaggtt gcgcgatggg cacgagagaa tcaagtccct
     1261 tatcttggtc tttgtttggg catgcaggtc atgtgcattg agtttgccag gcatatcctg
     1321 gggagcgatg agccgaacag caccgaattc gaccggaatt gcgagcatcc gatcatcagt
     1381 ctgatgcccg atcagcatga gctggccgac atgggtggga cgatgcgtct gggcgcttac
     1441 ccggcgattc ttaaacacgg cacgaaagct tatgctgcct atggggagga gaggatttcg
     1501 gagcggcatc gccatcgctg ggagttcaat aacgcctatc gggagctgtt gagcgccgcc
     1561 ggcatggtgt tcagtggtct gtcgcccaac ggtcgattgg tagagatctc cgagatgcgc
     1621 gaccatccct tcatgctggg tactcaattc catcccgagt tcaagagccg acctaatcgg
     1681 ccgcacccct tgttcaacgc cttcatggcc gcggccgttg attatcaagt ggccgctcaa
     1741 aagcaagagc aaaattccga cgagacgaat agcggatgat ggaacccatc gagggttcag
     1801 gacggacagg ccatcgccta ttctctcttt gtcagaatag gcccctgctc tccggttttt
     1861 tcacgtcgct taggctcttc cgcctctaac tgacgccatg cagaccggag tgattccagt
     1921 tcctcagccg tctcgggcac ttctccgtag cgcacgcgga tgaatgcttc ggtgattaga
     1981 cgggtctccg tcgtatactc cggccatacg cggctcagcg aagagaggta ctcatacgga
     2041 gtctcggagc cggagcgcgg atagcccgcc gctgtcgctg ctgcgcacat ctgccggtag
     2101 atgcgtcgaa cggaagcggc cgctcgccaa tgccggagaa ctcccagccg ttccagcagg
     2161 cggcggccga tgcccggctt ttcatcagac tcggcgatgg cccggctgtg ctcgctgtcg
     2221 cgggcggcaa atgtggcttg ccggtacgtt ctggccaggg cgagagcgat gatcaccagc
     2281 agaccgatca tgatcaggcc cgttgccgcc ttatcggtga aacctgaccc tattgcctcg
     2341 gccgtttcag tgggggtggg gattaacggg gaatccaggt c
//
LOCUS       DAOMQG010000475         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_18041_length_2345_cov_6.710044, whole genome shotgun sequence.
ACCESSION   DAOMQG010000475 DAOMQG010000000
VERSION     DAOMQG010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18041_length_2345_cov_6.710044"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..627)
                     /locus_tag="PLC06_16160"
     CDS             complement(196..627)
                     /locus_tag="PLC06_16160"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRO92035.1"
                     /translation="MNPLITDETRDRLIAAACAARPSAYVPYSHFSVGAALLADDGRV
                     YTGVNVENASYGLTVCAERNAIGAMALAGGRRVLAVAVCTANGVTPCGACRQVLSEFA
                     PDEGDAPVWIVDGAGGVRETSLAALLPDSFGPRHLHSRHDA"
     gene            complement(624..1691)
                     /locus_tag="PLC06_16165"
     CDS             complement(624..1691)
                     /locus_tag="PLC06_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRO92036.1"
                     /translation="MIAVVLLAAGQGVRMNSNKQKILHEVGGRPMVLHAFLAAEAVAD
                     LPPVLVVGPGEAGVRALLGDRARYVIQPEALGTGHATLMAAEALRGRAGQVVVTYGDM
                     PLLRAETLRALAESQRENGAAIALLSVIGEPNSSFGRVVRDELGRMREIVEVAEARRR
                     SEADELLAITELNAGVYCFDGEWLWANIGDLPIRRARRGPEYYLTDLIEMAVVAVPTD
                     DPDEGLGAGTRAEMVEVERAFRRRVNAHWLANGVTLVEPDATWIGPDVTIGRDTVIWP
                     NTFLQGRTTVGAECVIGPNAIVRDATVGDGCRIEQLLIEGLVVPDSTTARPGEWFPRL
                     PTGVATGVATGVATGLATGEV"
     gene            1954..>2345
                     /locus_tag="PLC06_16170"
     CDS             1954..>2345
                     /locus_tag="PLC06_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRO92037.1"
                     /translation="MDMKSSAAPGKPATIILDLDGELDASTYLDVIERVHQLYSDGAR
                     QLVIDLSDLSFLSSSGLVALHSAALVMRGEEPPGPELGWSAFHAIATDVERGFETCCK
                     LVNPQGRVRKTLEMTGFNAFLEVFDDTDA"
BASE COUNT          447 a          794 c          684 g          420 t
ORIGIN      
        1 gaatggcttc ctcttccaac tgctcagcaa ctgggccggg aaggtcgatg gttattggag
       61 ttgaaacaga tgtcatggta cccctatctt aacagcgagt tggttggggt tcaaatttct
      121 cttagttcgc gaactttact ttcttttgtc tatgctcgtt cacaggttaa tcaagaaagc
      181 agcatcatac atgacttagg cgtcatgacg actatgcaag tgccgcggcc caaagctatc
      241 cggcagcaat gccgctagcg acgtttcccg cacgccccct gccccatcca cgatccacac
      301 cggcgcatct ccctcatccg gcgcaaactc gctgagcacc tgacggcacg ccccgcaagg
      361 tgtcacccca ttggccgtgc agacggccac ggccagcacg cgccgcccac cggccagcgc
      421 catcgccccg atcgcgttgc gctcggcaca caccgtcagt ccataggacg cgttctccac
      481 gttgacgccg gtgtagacgc ggccgtcgtc ggccagcagc gccgcgccga cggagaaatg
      541 cgaatagggc acataggcgc tcggccgggc cgcacaggcc gcggcgatga gccggtcgcg
      601 ggtttcatca gtgatcagtg ggttcatact tcacctgtag ctaaacctgt ggccacacct
      661 gtggccacac ctgtggccac acctgtgggg aggcgaggga accattcgcc gggtcgggct
      721 gtcgtggagt cgggcacgac aagcccctcg ataagcaatt gctcgatgcg acagccgtcg
      781 cctacggtcg cgtcgcggac gatggcattg gggccgatga cgcactccgc gccgacggtc
      841 gtgcgccctt gcaggaaggt gttgggccag atgaccgtgt cgcggccgat ggtcacgtcc
      901 ggcccgatcc acgtcgcgtc cggttccacc agcgtcaccc cgtttgccag ccaatgggcg
      961 ttcacacgac gacgaaaggc gcgctcgacc tcgaccatct ccgcccgtgt acccgcgccc
     1021 aacccttcgt ccgggtcgtc agtcggcacc gcaaccacgg ccatctcgat cagatccgtc
     1081 aggtaatatt ccggtccacg ccgcgcccgg cggatgggca gatcgccgat gttggcccac
     1141 agccactcac cgtcgaagca gtagaccccg gcgtttagct ccgtgatggc cagcagttcg
     1201 tccgcttccg aacgtcgtcg cgcctcggcc acctcgacga tctcgcgcat gcggccgagt
     1261 tcatcgcgca cgacccgacc gaagctgcta ttcggctcac cgatgaccga cagcagggct
     1321 atggccgcgc cattctcgcg ctgcgattcg gccagcgccc gcagcgtttc cgcccgcagg
     1381 aggggcatat cgccgtaggt gacgacgacc tgcccggctc ggccgcgcaa tgcctcggca
     1441 gccattaggg tggcgtgccc cgttcccaac gcttccggct ggatgacgta gcgcgcccgg
     1501 tcgcccagga gcgcccgtac gcctgcctcg ccggggccga ccaccagaac cggcggcaaa
     1561 tcggccacgg cctcggcggc cagaaaggca tggagcacca tcggccgccc accgacttca
     1621 tgcaggatct tctgcttgtt ggaattcatc cgcacgcctt gcccggcggc gaggagaacg
     1681 acggcaatca tgacctaatc atacagaaag tgtccgcgaa ttacacattg aataagcgtg
     1741 gtagatttcg caggcccttt ttcaatatat tgtcgctcag aggcaggcga gcgtcctgac
     1801 cggcaattca cggccgccgc gcgcgtagag tggtaatgtg tatgccgccg tcaagatgat
     1861 gtgacggccg cctaatgatt ggctacaatt acatcctgtc agaaaccgta aatcaaccgc
     1921 tcagcggagt gatgccgcga ggagacaatc agcatggaca tgaagagcag cgccgcaccc
     1981 ggcaaaccgg ctaccataat ccttgatctc gacggcgaac tcgacgcctc gacctatctg
     2041 gacgtcatcg agcgtgtgca ccaactctat agcgatggcg cgcgacagct agtcatcgac
     2101 ctgtccgacc tctccttcct ctccagttcc ggcctcgtgg cgctccacag cgcggcgctg
     2161 gtgatgcgcg gagaagagcc gcccggcccc gaattgggct ggagcgcctt ccacgccatc
     2221 gccaccgatg tcgaacgggg cttcgagacc tgctgcaagc tggtcaaccc ccagggccgc
     2281 gtgcgcaaaa cgctggagat gaccggcttc aacgcgtttc tggaggtatt cgacgacacc
     2341 gacgc
//
LOCUS       DAOMQG010000476         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_18050_length_2344_cov_6.982525, whole genome shotgun sequence.
ACCESSION   DAOMQG010000476 DAOMQG010000000
VERSION     DAOMQG010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18050_length_2344_cov_6.982525"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLC06_16175"
     CDS             <1..690
                     /locus_tag="PLC06_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HRO92038.1"
                     /translation="RHADAVRNTLILFEAARQAGVRRVVHVSITNPSADSPLEYFSGK
                     ARLEQALIESGLSYAILRPTVLFGPEDILINNIAWMLRRLPIFGVFGDGSYKLQPIYV
                     DDLAGLAVQWGATRDNIVLNAIGPETFTYRELVETIGRLIGARRPVIGVPPAVGYWAG
                     WLVGKLKGDVTITREEITGLMDNLLYVETPPTGTTKLTDWVTAHADTVGRHYANELAR
                     RIDRTEAYQSP"
     gene            791..1669
                     /locus_tag="PLC06_16180"
     CDS             791..1669
                     /locus_tag="PLC06_16180"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRO92039.1"
                     /translation="MNDDTNWKDTLIHLFALASRLEGEGQYNLAKLMRAAADSLCRRE
                     TYPLDIPGDVDTLTAEVQQAIAALSGLDMNADLLATMTQGAAFMTQGKLSPITATPNP
                     WVCRGCGTVALSPPVEPCPVCGQWPETFQLFMPNYWFDALQPEAARERLRRTPLVVEE
                     LIAGLSDDALNRVPPDGGWAIRNTLSHLRDAQGVLDFRVDLFLQEAHPVLESKAVFAW
                     AKNESERPPSARDIFDTYRASRAQTLRKLESFAPFDWERTGEHEEFGTVTLRQQVSYF
                     AAHESTHLGQIQALIP"
     gene            1831..2217
                     /locus_tag="PLC06_16185"
     CDS             1831..2217
                     /locus_tag="PLC06_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRO92040.1"
                     /translation="MADTEKITINMSAVDLGKVDLLIQEGLYSNRTDFIRTAIRSRLD
                     KHGLEIQQSITRHSYMIGVWSYKRSDLEKHQQKGQKVTLTVLGILNLADDITPELARE
                     VIESVQVRGIFQASEKVKAALADRMK"
BASE COUNT          480 a          714 c          697 g          453 t
ORIGIN      
        1 cgccacgccg acgccgtgcg caacacgctg atcctcttcg aggccgctcg gcaggccggc
       61 gtgcggcgcg tcgtccacgt cagcatcacc aacccctcgg ccgactcgcc gctggagtat
      121 ttcagcggca aggcgcggct ggagcaggcg ctgatcgaga gcggcctctc ctatgccatc
      181 ctgcggccga cggtgctctt cggcccggag gacatcctga tcaacaacat cgcctggatg
      241 ctgcgccggc tgccgatctt cggcgtcttc ggcgacggca gctacaagct gcagccgatt
      301 tatgtggacg atctggccgg tctggccgtg caatggggcg cgacgcggga caacatcgtc
      361 ctcaacgcca tcggcccgga gaccttcacc tatcgcgagc tggtggagac catcggccga
      421 ctgatcggcg cgcggcggcc ggtgatcggc gtgccgccgg ccgtgggcta ctgggccggt
      481 tggctggtgg gcaagctgaa aggggacgtg accatcactc gcgaggagat caccgggctg
      541 atggacaacc tgctctacgt ggaaacgccg cccaccggca cgaccaaact gaccgactgg
      601 gtcacggcgc acgccgacac cgtggggcgg cactacgcca atgaactggc ccgccggatc
      661 gatcgcactg aagcgtatca aagcccatga tttgtgggct atatccccat attttcttgc
      721 aggaggtaac cctgtcaatc aaaagccacg attattatgt caataggtaa taaaccggga
      781 tagatgtttc atgaatgacg ataccaactg gaaagatacc ctcatccacc tcttcgctct
      841 cgcctcgcgg ctggaggggg aggggcaata caacctggcc aaactgatgc gcgccgcggc
      901 cgattcgttg tgccgccgcg aaacgtaccc gctcgacatt cccggcgacg tggacacgct
      961 gaccgccgaa gttcagcaag cgattgcagc cttgtccggc ctggatatga acgcggactt
     1021 gctcgccacg atgacccagg gcgcggcgtt catgacccag gggaagctct cgcccatcac
     1081 cgccacgccc aacccctggg tctgccgcgg gtgcggaacc gtcgcgctgt ctccgcccgt
     1141 cgagccgtgt cccgtctgcg gtcaatggcc ggagaccttt caactgttca tgcccaacta
     1201 ctggttcgac gcgctgcaac ccgaggcggc gcgggaacgc ctgcgccgga cgccgctcgt
     1261 ggtggaggaa ctcatcgcgg ggctgtccga tgacgctttg aatcgcgtgc cgcccgacgg
     1321 cggttgggcg atccgcaaca cgctgagcca tctgcgcgac gcccagggcg tacttgattt
     1381 tcgtgtagat ttgtttcttc aggaggcgca tcccgtcctc gaatcgaagg ccgtctttgc
     1441 ctgggcgaaa aacgagtcgg agcggccgcc ctcggcgcgc gacatcttcg acacctatcg
     1501 cgcctcgcgc gcgcagacgc tccgcaagct ggaatcgttc gcgccgttcg attgggagcg
     1561 cacgggcgag catgaggagt tcggcacggt gaccctccgc cagcaagtca gttatttcgc
     1621 cgcgcatgaa agcacgcatc tggggcagat tcaggcgttg atcccataac catattccgc
     1681 tcgtttcagg ttggcgactc cccatgaaca tcgaatttct gtgttaatcg agagaatcgt
     1741 agccaagatt agcacccaca tcccttgaca aaagccacat tctgcataaa atcatctata
     1801 taccatccat atggatgaca aggagatgat atggcagata ccgagaagat aaccattaac
     1861 atgagcgcgg ttgatttggg caaggtggac ttgctcatcc aggaaggact gtattcgaac
     1921 cgcacggact ttatccgcac ggcgatccgg tcacggctgg acaagcatgg cctcgagatt
     1981 cagcaatcga tcacccgcca ctcgtatatg atcggtgtat ggagctacaa gcgttccgat
     2041 cttgaaaaac atcaacaaaa ggggcaaaag gtcacgttga ctgttctggg gattctgaac
     2101 ctcgctgacg acatcacccc ggagttggcg cgcgaagtca ttgaatccgt tcaggtacgc
     2161 gggattttcc aggcatccga gaaggtcaaa gccgcgttgg cggaccgaat gaaatagcga
     2221 agtggcgagt gacgagtgac gaatgcgctc ttgctgtggt ctgtcgtctg cagtccgtgg
     2281 tctatgcgcg agtccggcaa tagctgttaa cgtgcgctct tccagacgac cgtgcatgat
     2341 cttt
//
LOCUS       DAOMQG010000477         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_18482_length_2303_cov_10.878559, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000477 DAOMQG010000000
VERSION     DAOMQG010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18482_length_2303_cov_10.878559"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLC06_16190"
     CDS             <1..291
                     /locus_tag="PLC06_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92041.1"
                     /translation="WQRSNPVERAVLAAVAHLMDRNEPLHPEEIVDYLQTYSIELNPA
                     ETTHALNALVEQDVMQEVTVEGKSLYELRIGLVGLWVVQNKSLSKLHVHLES"
     gene            complement(288..785)
                     /locus_tag="PLC06_16195"
     CDS             complement(288..785)
                     /locus_tag="PLC06_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRO92042.1"
                     /translation="MKTLLLMRHAKSSWDNSRLTDYERPLNERGRRDAPRMGRLLRHE
                     GLTPDLIITSSAKRAATTAELVALELALDSDTRYTEMLYLADPDAYIRQARQVGDDIE
                     ILLMVGHNPGIQELVEQLTGQEEEMSTAALAYIRAPVRKWSELAGGKQYELVHVWRPR
                     ELAGD"
     gene            complement(869..1534)
                     /locus_tag="PLC06_16200"
     CDS             complement(869..1534)
                     /locus_tag="PLC06_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRO92043.1"
                     /translation="MSNEMGFNPMLDDDDDDDFPEVEPIEEPGEDKPEDGKSKDDGLR
                     SLEDRLLKGRTILIYGPIDMNVAMDVTRRLLVMDAESQDEIRIFINSPGGHVESGDTI
                     FDMIRFVKSPVKVIGTGWVASAGALIYAAADLENRYSLPNTRFLLHQPAGGATGQASD
                     IAIEAQEIIKMRRRLNEIFAQQTGQSIEKVEEDTDRNFWMSASQAKEYGLVGHIVASA
                     DEI"
     gene            complement(1683..>2303)
                     /gene="eno"
                     /locus_tag="PLC06_16205"
     CDS             complement(1683..>2303)
                     /gene="eno"
                     /locus_tag="PLC06_16205"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942420.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRO92044.1"
                     /translation="LEAIKEAGYVPGEQIMLAMDPAASEFYEDGKYHLKVEGKTLTGR
                     EMVDFWESWVDKYPIISIEDGLHEDDWDNWALMVERLGDRLQIVGDDLLVTNVNRIQM
                     ALDRHAANSLLCKVNQIGTLTEAIESVNLVQRNGWTAVVSHRSGESEDATISDLVVAL
                     NAGQIKTGAPARSDRVAKYNQLLRIEEDLGDAAIYPGMKAFSNLRH"
BASE COUNT          496 a          695 c          598 g          514 t
ORIGIN      
        1 tggcagcgat caaaccctgt tgaacgagca gtattggctg ccgtcgccca cttgatggat
       61 cgtaacgagc cactgcaccc ggaagagatc gtcgattatt tgcagacata cagcatcgaa
      121 ctgaacccgg ccgagaccac ccacgccctc aacgccctgg tcgaacagga cgtgatgcag
      181 gaagtcaccg tggaaggaaa atcgctctat gagctaagga tcggtctggt cggattgtgg
      241 gtggtgcaaa acaagagcct gagcaaactc cacgttcacc tggaaagtta atctcccgcc
      301 aattctcttg gccgccacac gtgcactaac tcgtattgtt ttcccccggc aagttccgac
      361 catttcctga caggcgcacg aatataagcc agcgccgccg tgctcatctc ctcttcctgg
      421 cctgtcagct gttccaccaa ctcctggata ccggggttat gtcccaccat caggagtatc
      481 tcgatgtcgt cgcccacctg ccgtgcctgc ctgatatatg catcagggtc agccagatag
      541 agcatttcgg tataacgggt gtccgagtcg agcgcaagtt cgagagcgac aagctcggcc
      601 gtcgtcgccg cgcgcttcgc cgaagatgtg atgatgagat caggggtcaa gccttcatgt
      661 cgcagaagcc ggcccatgcg gggtgcgtca cgccggccgc gttcgttcag cggccgctcg
      721 taatcggtta gccttgaatt gtcccagctc gatttcgcat gtcgcattag caataaggtc
      781 ttcatgcctc tcctgttccc catctcatga aaacaaaagg ggcacccgga agcgcccctt
      841 gaattccgcc catgatatca tgcgcgctct agatctcgtc ggccgacgcg acgatatggc
      901 caaccaggcc gtactcttta gcctgcgaag ccgacatcca gaaattgcgg tcggtatcct
      961 cttccacctt ctcgattgat tgcccggtct gttgggcaaa aatctcgttc aggcgacgac
     1021 gcatcttgat gatctcctgc gcctcgatgg cgatatctga agcctgccct gtggccccac
     1081 ccgcgggctg atgcaacagg aagcgagtgt tcggcaaact gtagcgattc tccaaatcgg
     1141 ccgcggcata gatcaatgcc ccggcgctgg ccacccagcc cgtaccgatc accttgaccg
     1201 gagacttgac gaagcggatc atatcgaaga tcgtgtcccc ggactccaca tggccgcccg
     1261 gcgaattgat gaaaatacgg atctcgtcct gcgattcggc atccatcacc agaagtcttc
     1321 gggtgacgtc catagcgaca ttcatatcaa tagggccata gatcaggata gtgcggccct
     1381 tcagcaagcg gtcttccaac gaacgtaggc cgtcgtcctt gctcttgcca tcttccggct
     1441 tgtcttcacc gggttcctcg atgggttcga cttcggggaa gtcgtcatca tcgtcatcat
     1501 ccagcatagg gttgaacccc atttcattgc tcatgcgtgc gattctcctc aggacttctc
     1561 gttagtcctt gtgaggttca ttataagaaa tcctgacgat atatgcaccc cgggcattcg
     1621 ccgaagatat tcaacgaata cccaagggtg gcaaaatcat acaggaacca gatatcttga
     1681 ttctagtggc gcaaattgga gaaggctttc atgccggggt agatcgccgc gtcgcccagg
     1741 tcttcctcaa ttcgcaggag ctggttgtac ttggcaacgc ggtcgctgcg agccggggca
     1801 ccggtcttga tctggccggc attgagcgcc acgaccaggt cagagatggt cgcgtcctcg
     1861 gactcgccgc tgcgatggga aaccacggcc gtccagccat tacgctgaac cagattcact
     1921 gattcaatcg cttcggtcag agtgccgatt tgattaacct tgcagagcaa gctattggca
     1981 gcgtggcgat ccagagccat ctgaatacga ttcacgttgg tcacaagcaa gtcatcgccc
     2041 acgatctgca ggcgatcgcc caaccgctca accatcaacg cccagttatc ccagtcgtcc
     2101 tcatgcaggc cgtcctcgat ggaaatgatg gggtatttat cgacccagga ctcccagaag
     2161 tcgaccatct cgcggccggt cagcgtcttg ccttcgacct tcaaatgata cttgccgtcc
     2221 tcgtaaaatt cagaagcggc cgggtccatg gccaacatga tctgctctcc ggggacgtac
     2281 ccggcttcct tgatcgcttc cag
//
LOCUS       DAOMQG010000478         2293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_18572_length_2293_cov_6.966488, whole genome shotgun sequence.
ACCESSION   DAOMQG010000478 DAOMQG010000000
VERSION     DAOMQG010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18572_length_2293_cov_6.966488"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1188)
                     /locus_tag="PLC06_16210"
     CDS             complement(133..1188)
                     /locus_tag="PLC06_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRO92045.1"
                     /translation="MTTMLAAVYHGPNDLRVEKVPVPEIGPDELLLRVLRANICGTDL
                     RILHGGHRKYPPGTTRIPGHEVVGEVAATGAAVAADYPLGRRLFVAPNMGRGDSRETI
                     SGNNNLDPEYEAFGITLDGAFAEYMRVPAAAVRQGNLMGVAEGVDPAVAALVEPLACV
                     WRGQNKIAVGAGDVALVMGAGPIGVLHVMLARLRGATRIIVSEPAAGRREQALALGAD
                     VVVDPMNEDLAAVVAAESDGRGADVIITAAPVKALQEQALDLAAMGGRVNYFGGLAKD
                     DPFIRFNSNTVHYKELIVTGTTACSTYDCLRAAEIVNSGRLDLSPLITAAYPLSEVVA
                     AFAAAADGKNLRVTLIP"
     gene            complement(1253..1699)
                     /locus_tag="PLC06_16215"
     CDS             complement(1253..1699)
                     /locus_tag="PLC06_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRO92046.1"
                     /translation="MSSPYVYSFRELIVYQKARSLAAAVFTASKAFPRDEMYSLTDQI
                     RRSSRSIGANIAEAWAKRAYEKHFISKLTDADGEQLETQHWLEIGRDCGYLSAQQAAE
                     LMGRCEEVGRLLGKMISQADSFCRSTTLRESSPEYFVTPDDTQLLD"
     gene            complement(1817..>2293)
                     /locus_tag="PLC06_16220"
     CDS             complement(1817..>2293)
                     /locus_tag="PLC06_16220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRO92047.1"
                     /translation="RWLAASTPLRMYVEVANFKGAERGVTSENYYDNMFALVKFESGA
                     MASISGVCPCDYGYDSRVEIVGTKGIMQIGELKGESIVVCTDRDHGLVTPIFRRWPDR
                     FRWGYIRELEHFVDCVRAERHPIVTGEDGRWAVAMVLAGTRSFLEERAVYLSEVMD"
BASE COUNT          416 a          770 c          692 g          415 t
ORIGIN      
        1 gtggaggtgg ctatgggcat cgaccacgcc cagcgcttcg ggcgataccg ggccgaggac
       61 ggtcatgact gtgttatcag acatcaaaga acattccaaa tctgtgcaat ctgtaaaatc
      121 tgtggattct ccctatggta tcaacgtcac ccgcagattc ttcccgtcgg ccgcggcggc
      181 gaaggcggcg accacctcgc tcagcggata ggcggcggtg atcagcggcg acagatcgag
      241 ccggccgctg ttgacgatct cggccgcgcg cagacaatcg taggtgctgc acgccgtcgt
      301 gccggtgacg atcaactcct tgtaatggac cgtgttgctg ttgaaacgga tgaacgggtc
      361 gtccttggcc aacccgccga aatagttgac gcggccgccc atcgccgcca gatcgagggc
      421 ctgctcctgc aacgccttca ccggcgcggc agtgatgatg acgtcggccc cgcggccgtc
      481 gctctcggcg gccacgacgg ccgccaggtc ttcattcatc gggtcaacca ccacgtcggc
      541 ccccagcgcc agcgcctgct cgcgccgccc ggcggccggc tcgctgacga tgatccgcgt
      601 cgccccgcgc agccgggcca gcatcacatg cagcacgccg atcggccccg cgcccatgac
      661 cagcgccaca tcgcccgcgc cgacggcgat cttgttctgc ccgcgccaga cgcaggccag
      721 cggctccacc agcgcggcca cggccgggtc gacgccttcg gccacgccca tcaggttgcc
      781 ctggcggacg gccgcggcgg gcacgcgcat gtactcggcg aacgcgccgt caagggtgat
      841 gccgaacgcc tcgtactccg ggtcgaggtt gttgttgccg ctgatggtct cgcggctgtc
      901 gccccggccc atgttggggg ccacgaacag gcggcggccg agcgggtaat ccgcagccac
      961 agccgccccc gtcgccgcca cctcacccac gacctcgtgg ccggggatgc gcgtcgtgcc
     1021 cggcgggtat ttgcggtggc cgccatgcag gatgcgcagg tcggtgccgc agatgttggc
     1081 ccgcagcacg cggagcagca gctcgtccgg cccgatctcg ggcacgggaa ctttttctac
     1141 acgaagatca ttggggccgt gatagacggc ggcgagcatt gtggtcattg gtgttactct
     1201 gtgttcagta ggtcagtagg tcagtgggtc ggtaggtcag tggggcagca ggtcagtcga
     1261 gtagttgggt gtcatcgggg gtgacgaaat attcgggcga tgattcgcgc agggtggtag
     1321 agcggcaaaa ggaatcggcc tggctgatca ttttgcccag caggcggccg acttcttcgc
     1381 aacgacccat cagttctgcc gcttgttgag ccgacagata cccgcaatcg cggccgatct
     1441 ccagccagtg ctgtgtctcc agctgttccc cgtcagcatc ggtcaatttg ctgatgaaat
     1501 gtttttcata cgcacgcttg gcccaggcct cggcgatatt cgcgccgatc gaccgcgacg
     1561 atcggcggat ctgatcggtc agcgaataca tctcatctcg cggaaaagct ttcgatgccg
     1621 taaacacagc cgcagccaat gagcgtgcct tctgatatac aatcagctct cgaaaactat
     1681 acacatacgg cgacgacata ccaccacctc cacatcccga ctaccttact gatccactga
     1741 ccactgatcc actgaccact gatccactga tccactgacc actgatctac tgacctactg
     1801 atctactgat ccactgctaa tccatcactt cactcaaata aaccgcccgc tcctcaagaa
     1861 acgacctcgt ccccgccagc accatggcca cagcccagcg gccgtcctcg ccggtgacga
     1921 tggggtggcg ttcggcgcgg acgcaatcga cgaaatgctc cagctcgcgg atgtagcccc
     1981 agcggaagcg gtcgggccag cggcggaaga tgggcgtcac cagcccgtgg tcgcggtcgg
     2041 tgcacacgac gatcgactcc cccttcagct cgccgatctg catgatcccc ttcgtgccga
     2101 cgatctccac ccggctgtca tagccgtagt cgcaggggca aacgccgctg atgctggcca
     2161 tcgccccgga ctcgaacttg accagagcga acatgttgtc gtagtagttc tcgctggtca
     2221 cgccgcgctc ggcccccttg aagttggcga cctcgacgta catgcgcagc ggcgtggaag
     2281 cggccagcca gcg
//
LOCUS       DAOMQG010000479         2285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_18654_length_2285_cov_8.241704, whole genome shotgun sequence.
ACCESSION   DAOMQG010000479 DAOMQG010000000
VERSION     DAOMQG010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18654_length_2285_cov_8.241704"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLC06_16225"
     CDS             <1..714
                     /locus_tag="PLC06_16225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRO92048.1"
                     /translation="TIRINNVNFRVIGLMEEKGGSGFNDQDNTILVPLNTAQRRLFPA
                     RRPDGKFRIDQIYAQVISEERQDAAITEMELALRQTRGIAFRDEDDFTILSQSEIIGA
                     LGQITGVLTTFLGVIAGISLLVGGIGIMNIMLVSVTERTREIGLRKAVGARRSNILWQ
                     FLTEAVILSSIGGLIGLALGATGAWLISLLSDTLDATVAWNSVVLAILFSAAVGLFFG
                     IYPATRAAALNPIDALRYE"
     gene            782..2026
                     /locus_tag="PLC06_16230"
     CDS             782..2026
                     /locus_tag="PLC06_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO92049.1"
                     /translation="MKLLENIRIALNGLSANKLRAALTMLGIMIGVAAVITLLALGDG
                     VTRFVADQFSGLGTNLVFILPGGEGPGPMRDSGPVAQMTLRDAELLADPGLVPGVKAV
                     APVVFNQVDLQYGGNLHNIMARASTPNYADISNLEIARGRAIDDHDYAARSRVVVLGP
                     DTVKAIFPDDVDPLDQDIRVNGITFRVIGVLTEKGAGGMGGSQDDTALIPLTTAQERL
                     FDSRSATGDYLIDAVLMQAASDKAIDGIIIDASTVLRQSHNIAFRDDDDFQILTQGDF
                     IQAFGAVTSVLTLFLGAIAGISLLVGGIGIMNIMLVSVTERTREIGLRKAVGARRRDI
                     LGQFLTEAVVLAVLGGALGIVIGVLGATAINLYVPELDTSVTVNSVALAVGFSVAVGL
                     FFGIYPASRAAGLHPIEALRFE"
     gene            2031..2228
                     /locus_tag="PLC06_16235"
     CDS             2031..2228
                     /locus_tag="PLC06_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92050.1"
                     /translation="MKKRDWRAFFRRLILAAAVAFFIAVAPLPAWTPGWFLYWQVPAT
                     IFVFIAYMGKVLIDTILFPKR"
BASE COUNT          397 a          736 c          728 g          424 t
ORIGIN      
        1 accattcgca tcaacaacgt caatttccgc gtcatcggcc tgatggaaga gaaaggcggc
       61 tccggcttca acgatcagga caacaccatc ctcgtgccgc tcaacaccgc ccagcgccgc
      121 ctcttccccg cccgacggcc cgacggcaag ttccgcatcg atcagatcta cgcccaggtg
      181 atcagcgagg agcggcagga cgcagccatc accgagatgg aactggccct tcgccagacg
      241 cgcggcatcg cctttcgtga tgaggacgac ttcaccatcc tgagccaatc ggagatcatc
      301 ggcgcgctgg gacagatcac cggcgtgttg accaccttcc tgggcgtcat cgccggcatc
      361 tcgctgctgg tgggcggcat cggcatcatg aacatcatgc tggtcagcgt gacggagcgc
      421 acccgcgaga tcggcctgcg caaggcggtc ggtgcgcgac gctccaacat cctatggcaa
      481 ttcctgaccg aggccgtcat cctgtccagc atcggcggcc tgatcggtct ggcgctgggc
      541 gcgaccgggg cctggctcat cagcttgtta tcggacacgc tggatgccac cgtcgcctgg
      601 aactcggtcg tgctggccat cctgttctcg gccgccgtgg gtctgttctt cggcatttac
      661 ccggccaccc gcgcggccgc gctgaatccg atcgatgcgt tgcgctatga gtaggggaaa
      721 aggggaaggg gagaaggggg gaaagggagc aggggtgaaa gggagcaaga ggtaaacggg
      781 aatgaagctg ctcgaaaaca tccgcatcgc cctcaacggg ttgtcggcca acaaactgcg
      841 ggccgccctg accatgctgg gcatcatgat cggcgtagcc gccgtcatca ccctgctggc
      901 cctcggcgac ggtgtgaccc gctttgtggc cgaccagttc tcagggctgg gcacgaacct
      961 cgtcttcatc ctgcccggcg gcgaggggcc ggggccgatg agagattccg gcccggtggc
     1021 ccaaatgacc ctgcgcgatg ccgagctgct ggccgacccc gggctcgtcc ctggcgtcaa
     1081 ggccgttgct ccggtcgtgt tcaatcaggt tgacctgcaa tatggcggca atctccacaa
     1141 catcatggcc cgcgcctcga cgcccaacta cgccgatatc agcaatctgg agatcgcccg
     1201 cggccgcgcc atcgacgacc acgactacgc cgcccgttcg cgggttgtcg tcctcggccc
     1261 cgatacggtc aaggcgatct tccccgacga cgttgacccg ctcgatcagg acatccgcgt
     1321 caacggcatc accttccgcg tcatcggcgt gctgaccgaa aagggagccg ggggcatggg
     1381 cggcagccag gacgacacgg cgctcatccc gctgacgacc gcccaggagc gcctcttcga
     1441 tagccgcagt gccaccggcg actacctgat cgatgcggtg ctcatgcagg cggcgagcga
     1501 caaggccatc gacggcatca tcatcgacgc ctcgaccgtg ctgcgccaga gccacaacat
     1561 cgccttccgc gacgacgacg acttccagat tctgacccag ggcgatttca tccaggcctt
     1621 cggcgcggtg acgagcgtgc tgacgctctt tctgggggcc atcgcgggta tctcgctgct
     1681 ggtggggggc atcggtatca tgaacatcat gctcgtcagt gtgacggagc gcacgcgtga
     1741 gatcggcctg cgcaaggcgg tgggcgcgcg gcgacgcgac atcctgggcc agttcctgac
     1801 cgaggcggtg gtgctggctg tgctgggcgg ggcgctaggc atcgtcatcg gcgtgctggg
     1861 cgcgacggcg atcaatctct atgtgccgga gctggacacg tcggtgacgg tcaactcggt
     1921 ggcgctggcc gtcggctttt ccgtggccgt ggggctgttc ttcggcatct atcccgcctc
     1981 gcgcgcggca gggctgcacc cgattgaggc gttgcggttt gaataggacg atgaaaaaac
     2041 gagactggcg ggcattcttc cggcggctaa tcctggccgc ggccgtggcc ttcttcattg
     2101 ccgtggcccc actgcccgcg tggacgcccg gctggttcct ctattggcag gtgccggcga
     2161 cgatttttgt cttcatcgcc tatatgggca aggtgctgat cgatacgatt ttgtttccga
     2221 agagataatg atttgataat ctcttgcgcc aatccggcat gcgcaacctt accccatcgg
     2281 agtta
//
LOCUS       DAOMQG010000480         2241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19057_length_2241_cov_6.389753, whole genome shotgun sequence.
ACCESSION   DAOMQG010000480 DAOMQG010000000
VERSION     DAOMQG010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19057_length_2241_cov_6.389753"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLC06_16240"
     CDS             <1..347
                     /locus_tag="PLC06_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRO92051.1"
                     /translation="RPLDEIAAEAESWAAQLRGRGIAATTAPDESYVGGGSLPGESLP
                     TRVVVVDPGAMSAGELAARLRAAPLPVIGRIHDGRLRLDPRTVLPGQEEGLLRAVISG
                     TVGQWDSGTVGQ"
     gene            531..1535
                     /gene="tsaD"
                     /locus_tag="PLC06_16245"
     CDS             531..1535
                     /gene="tsaD"
                     /locus_tag="PLC06_16245"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRO92052.1"
                     /translation="MTRILAIETSCDETAAAVIADGTQILSSVVASQSDLHARYGGVF
                     PEVASRQHVEVIHAVVEEAMRVAHLGFDDLDCVAVTRGPGLVGSLLVGVNMAKGLAVS
                     RNLPLLGVNHIEGHIYSLWLTEDAPHIHFPLVTLVVSGGHTDLYLMTDHGKYQLLGAT
                     LDDAAGEAFDKVGRLLGLPYPGGPAIDKASEHGSSTTFRFPRAVMDVNHGYDFSFSGL
                     KTAVMRQAGQYHSTATMPVADLAAGFQAAVVESLVEKTAAAAEEFGATAVHVVGGVSA
                     NRALRAAMRARLELPVRFPPMALCTDNAAMIGAAAHFNYIRGRRDGLDLDVTPNLQLT
                     "
     gene            1532..>2241
                     /locus_tag="PLC06_16250"
     CDS             1532..>2241
                     /locus_tag="PLC06_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRO92053.1"
                     /translation="MSSETAPLPHMDHTGLAINADARFRHVFAEGFDPILLTDHLGRV
                     LDGNRQALNYFGIDRRAFPQINIRDLHHPDEPLPDVNKLTGPGSMSFHSRVVAERAID
                     GRRRQMTLNVEVRARRLTPGREGTWQWIYHDVSSRVELQQMRDDLIAMLLHDLQSPLG
                     NVISTIELLRMRAESTEPDETLLSLLDIAARSSGQLQRLIASLMDINRLEAGQPVGRR
                     SAVALADLVREAYEIEEP"
BASE COUNT          384 a          787 c          732 g          338 t
ORIGIN      
        1 cccgaccgct cgacgagatc gccgccgagg ccgagagctg ggccgcgcag ctgcgcggcc
       61 gcggcatcgc cgcaaccacc gcgccggacg agtcatacgt cggcggcggc agcctgcccg
      121 gcgagagcct gccgacgcgg gtggtggtcg tcgatcccgg cgcgatgagc gccggcgaac
      181 tggccgcccg cctgcgcgcc gcgccgctgc cggtcatcgg ccgcatccac gacggccgcc
      241 tgcgcctcga cccgcgcacg gtgctgccgg ggcaggaaga gggattgctg agggcggtga
      301 tcagtgggac agtgggacag tgggacagtg ggacagtagg acagtaggac agtagatcag
      361 taggacagta ggacagtaga tcagtaggac agtaggacag taggacagta ggacagtaga
      421 tcagtattca gcaggtcagt ggccggtggc cggtggccga aagccggcaa acaggaagat
      481 tgctctttat tcgtaattcg taattcgtaa ttcgtaattc gtaattaatc atgacccgca
      541 ttctcgccat tgaaacatcc tgcgacgaaa cggccgcggc ggtcatcgcc gacggcacgc
      601 agattttgag cagcgtcgtc gcttcgcaat ccgatctcca tgcccgctac ggcggcgtgt
      661 tccccgaagt cgcctcgcgc cagcacgtcg aggtcatcca cgccgtggtc gaggaagcga
      721 tgcgcgtggc ccatctgggc ttcgacgacc tcgactgcgt ggccgtgacc cgcgggccgg
      781 ggctggtcgg ctcgctgctg gtgggggtca acatggccaa ggggctggcc gtcagccgga
      841 atttgccgct gctgggcgtc aaccacatcg agggccacat ctactcgctg tggctgaccg
      901 aggacgcgcc ccacatccac ttcccgctcg tcaccctcgt ggtcagcggc ggccacaccg
      961 acctgtacct gatgaccgac cacggcaaat atcagctgct gggggccacg ctcgacgacg
     1021 ccgcgggcga ggccttcgac aaggtcggcc gcctgctggg gctgccctat cccggcggcc
     1081 cggccatcga caaggcctcg gaacacggct catccactac cttccgcttc ccgcgcgcgg
     1141 tgatggacgt caaccacggc tacgacttca gcttcagcgg cctcaagacg gccgtcatgc
     1201 gtcaggcggg ccaatatcat tcgaccgcga ccatgcccgt ggccgacctg gccgccggct
     1261 tccaggcggc cgtcgtcgag tcgctggtgg agaagacggc cgccgccgcc gaggagttcg
     1321 gggccactgc cgttcacgtc gtcgggggcg tgtcggccaa tcgggcgttg cgcgccgcca
     1381 tgcgcgcccg gctggagctg ccggtgcgct tcccgcccat ggccctctgc accgacaacg
     1441 cggccatgat cggcgcggcc gcccatttca actacattcg cggccgccgc gacggcctcg
     1501 acctggacgt gacgcccaat ttgcaactga catgagcagc gaaaccgcac ccctgcccca
     1561 catggatcac accgggctgg cgatcaacgc cgacgcccgc tttcgccacg tgttcgccga
     1621 agggttcgac cccatcctgc tgaccgacca tctcggccgc gtcctcgacg gcaaccgcca
     1681 ggcgctcaac tatttcggca tcgaccggcg cgccttcccg cagatcaaca tccgcgacct
     1741 tcaccacccc gacgagccgc tgcccgacgt caacaagctg accggccccg gctcgatgag
     1801 cttccacagc cgcgtcgtcg ccgagcgggc catcgacggc cgccggcggc agatgacgct
     1861 caacgtcgag gtgcgcgccc gccggctgac ccccggccgc gaagggacgt ggcaatggat
     1921 ctaccacgac gtctccagcc gggtggagct gcagcagatg cgcgacgacc tgatcgccat
     1981 gctgctccat gacctgcaaa gcccgctggg caacgtcatc tccaccatcg agctgctgcg
     2041 gatgcgcgcc gagtcaaccg agccggacga gacgctgctg agcctgctcg acatcgccgc
     2101 ccgcagtagc ggccaactcc agcggctgat cgcctcgctg atggacatca accgcctgga
     2161 agcggggcag ccggtggggc ggcggtcggc cgtcgcgctg gccgatctgg tgcgcgaggc
     2221 ctacgagatc gaggagccga g
//
LOCUS       DAOMQG010000481         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19075_length_2240_cov_5.718993, whole genome shotgun sequence.
ACCESSION   DAOMQG010000481 DAOMQG010000000
VERSION     DAOMQG010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19075_length_2240_cov_5.718993"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLC06_16255"
     CDS             complement(<1..1157)
                     /locus_tag="PLC06_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92054.1"
                     /translation="MTDGPTTNPKTRPTTNSKAGPPPRRSRKAQAPLMLLAALSLLAG
                     LWAGLNRLGWVIPPLPGTITANHGPLMVNGFLGTVISLERAVALGRNLNRRRYLIAPI
                     LSAAGAAMLILSAPPFLARGLAVLAALALVGIFITIYRLQPGTDAAVMGLAAALWAVG
                     NLLWLAGTPIARVVPWWAAFLILTIAGERLELARVLLVGKGARQTFIAAVGITTAGLL
                     LSLVWPNAGIRIAGVGLVALGLWLLRFDIARRTIRRDGLTRFIAACLLPGYVWLTLGG
                     LVWAVYGTTYSSGPIYDALLHTLFLGFVMSMIFGHAPVIIPSVMGVKVPYTPAFYAHL
                     ILLHVSLVVRVAGDLLSSPALRQWGGLFNEIAILLFLAVTIISARFLGRKK"
     gene            complement(1154..1513)
                     /locus_tag="PLC06_16260"
     CDS             complement(1154..1513)
                     /locus_tag="PLC06_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HRO92055.1"
                     /translation="MNAQINLQRAYEAPNPDGGRRFLVDRLWPRGVARVNLALDGWFK
                     DVAPSNELRKWFGHDPARWDEFQTRYRHELDANRVAVQPLLDAAAAGKITLVYGARDT
                     DHNEAVVLRDYLLEHMP"
     gene            complement(1608..1838)
                     /locus_tag="PLC06_16265"
     CDS             complement(1608..1838)
                     /locus_tag="PLC06_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HRO92056.1"
                     /translation="MSEETILDIRPLPPMQRHPLIFNTFEALNSGQTFVLVNDHDPKP
                     LYYQFQAERTGEFTWDYLQQGPREWRVRIGKN"
     gene            complement(1855..>2240)
                     /locus_tag="PLC06_16270"
     CDS             complement(1855..>2240)
                     /locus_tag="PLC06_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92057.1"
                     /translation="SQGRSIMTVLNPADAIVVAMASAAAARLRPNAFSEPRPEEDAYL
                     ALQQYLADHFPSVPADILDIGPGSAERKAALKTALQQSGAVDDPLVRQGAAHVARLVA
                     TADPNAVTAVFDQPDDLVQATQITQ"
BASE COUNT          424 a          731 c          751 g          334 t
ORIGIN      
        1 tccttcttgc ggccgaggaa gcgcgcggag atgatcgtca ccgccaggaa cagcaggata
       61 gctatctcat tgaacagccc gccccactgc cgcagcgccg gcgacgaaag cagatcgccc
      121 gccacgcgca ccaccagcga gacatggagc aaaatcaggt gagcgtagaa ggcgggggta
      181 tagggcacct tgacgcccat gaccgaggga atgatcaccg gcgcgtggcc gaagatcatc
      241 gacatgacga accccaggaa tagcgtgtgc agcagcgcat cgtagatcgg ccccgaacta
      301 taggtcgtgc catagacggc ccagaccagc ccgccgagcg tcagccacac atagccgggc
      361 aacaggcagg cggcgatgaa gcgggtcagc ccgtcgcgcc ggatggtgcg ccgggcgatg
      421 tcgaagcgca gcagccacag acccagcgcc accagcccca cgcccgcaat ccgaatgccc
      481 gcgttgggcc acaccagcga cagcagcaga ccggccgtcg tgatgcccac ggcggcgatg
      541 aacgtctgcc gcgccccctt gcccaccagc agcacccgcg ccaactccag tcgctccccg
      601 gcaatagtca atatcaggaa ggccgcccac catggcacga cgcgcgcgat gggcgtgccc
      661 gccagccaca gcaaattgcc gaccgcccac agcgcggccg ccagccccat cacggcggcg
      721 tcggtgcccg gctgcaagcg atagatggtg atgaagatgc ccaccagcgc cagcgccgcc
      781 agcaccgcca gcccgcgcgc caaaaacggc ggggccgaca ggatcaacat cgcggccccc
      841 gcggccgaca ggatcggggc gatcaagtag cggcgacggt tcagattgcg gcccagcgcc
      901 accgcccgct ccagactgat gaccgtgccc aggaagccgt tgaccatcag cggcccgtgg
      961 ttggcggtga ttgtgcccgg cagcggcggg atgacccagc ccaaacgatt caacccggcc
     1021 cacaacccgg ccagcaacga cagggcggcc agcagcatca gcggcgcctg ggccttgcgg
     1081 ctgcggcgcg gcggcgggcc ggctttggag ttggtggtgg ggcgggtttt cgggtttgtc
     1141 gttggaccgt cggtcacggc atatgctcca gcagatagtc gcgcaggacg acggcctcgt
     1201 tatggtccgt gtcgcgcgcg ccatagacga gggtgatctt acccgcggcg gcggcatcga
     1261 gcaacggctg cacggcgacg cggttggcgt ccagctcatg gcgatagcgc gtctggaact
     1321 cgtcccagcg ggcggggtca tggccgaacc atttgcgcag ctcattgctg ggggccacgt
     1381 ccttgaacca gccgtcgagc gccaggttca cgcgggccac gccgcgcggc cacagccggt
     1441 cgaccagaaa acggcgtccg ccgtcggggt tgggcgcctc ataggcgcgc tgcagattga
     1501 tttgagcgtt catgttgtgg gtccgatggg ttgaaacggg cagacgacgg ccgcgagact
     1561 ctcccgcggc cgccgcccgt caagctcttc tcctcccgtg gagcagctta atttttgccg
     1621 atgcgcaccc gccactcgcg cgggccttgc tgcagataat cccaggtgaa ctcgccggtg
     1681 cgctccgcct ggaactgata gtacagcggc ttcgggtcgt ggtcgttcac gaggacaaac
     1741 gtctgccccg aattgagcgc ttcaaaggtg ttgaagatca ggggatggcg ctgcatgggc
     1801 ggcaacggcc ggatatcgag aattgtttct tcactcataa tcaaaaaacc tccgttattg
     1861 ggtgatttgg gttgcctgaa ccagatcgtc gggctgatca aagacggccg taaccgcgtt
     1921 ggggtcggcg gtggccacca gacgggcgac gtgggccgcg ccctgccgga cgaggggatc
     1981 atcgactgcg ccgctctgtt gcagagcggt cttcaacgcg gccttgcgct cggccgagcc
     2041 ggggccgata tccagaatat cggcgggcac ggacgggaaa tggtcggcca ggtactgctg
     2101 gagcgccagg taggcgtcct cttcaggtcg cggttcggaa aaagcgttgg gccgaagtcg
     2161 ggcggcggcg gcggaagcca tggccaccac gatggcgtcg gcaggattca gtacggtcat
     2221 gattgacctc ccttgcgaat
//
LOCUS       DAOMQG010000482         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19180_length_2230_cov_8.910345, whole genome shotgun sequence.
ACCESSION   DAOMQG010000482 DAOMQG010000000
VERSION     DAOMQG010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19180_length_2230_cov_8.910345"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLC06_16275"
     CDS             <1..1307
                     /locus_tag="PLC06_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92058.1"
                     /translation="GYGQIPLPSWIYIALFGIFLLSVAGYGIAGVKGRKEERVKGVLR
                     AALLPGSAAPPLMLLALNVAIFFAVLFNYLLVSPAGPMGRFFFPALSSLAILMFYGLS
                     LLVLTADRKRPTVGAVALVTNLFMFAISLIALFGYLAPAYARPPEYAAGATLPNATDV
                     RFDSFVALRGYAVDTDAARPGEPIGIELYWEVLATPPGDYLLFVHLTDDETGALVAQR
                     DTHPGLGNFPSSQWRPGDRFVERMKMYLPETAYAPSAGELTIGLYAPEGYRLGVTDNA
                     TGEGLGDAYPIGRVTINSQAAPDGEGLPNAGDYNFEDRIRLLGYDYDRRVLGVGEPIG
                     LTPYWEALRPSPGNYEVELAVLDNDNNVVFSQTDPLNSADWQPGEVVVGSYTLPTGTT
                     TGDLRVRLVLLDTTIGRRVNRVGDDGRWIDDELFLSTVAVRD"
     gene            1547..1795
                     /locus_tag="PLC06_16280"
     CDS             1547..1795
                     /locus_tag="PLC06_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92059.1"
                     /translation="MDYGMIGKIEKAKFYAEERDTRIVFQSLHVSILGDNDKTHQVKY
                     DNGLWHCDCNYFGSHQVCSHTMAMERILGSMVEMGIPS"
     gene            1900..2220
                     /locus_tag="PLC06_16285"
     CDS             1900..2220
                     /locus_tag="PLC06_16285"
                     /inference="COORDINATES: protein motif:HMM:NF028177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRO92060.1"
                     /translation="MEYVTRPSDHAGVRADKFYKTTLFRGDALMLGVNCLEPGQTQPA
                     HDHADQDKFYYVIEGAGRFQVGGEFITAGEGQLVWAAAGVVHGVSNESDTRLTLLVGI
                     APAP"
BASE COUNT          431 a          646 c          691 g          462 t
ORIGIN      
        1 tcggctatgg gcaaatcccg ctgccgtcgt ggatctacat cgcgttattc ggcatattct
       61 tgttgtctgt cgccggttat gggattgcag gggtgaaagg gagaaaggag gagcgggtga
      121 agggggtttt gcgtgcggcc ctgctccccg gttccgcggc tccccctctc atgctcctgg
      181 cgcttaacgt ggctatcttc ttcgccgtcc tgttcaacta cctgctggtc agcccggcag
      241 gaccgatggg acgcttcttc tttccggcgc tatcgtcgct ggcgatcctg atgttttatg
      301 ggttgagtct gctggttttg accgccgacc gcaaacggcc aaccgtcggc gcggtcgcac
      361 tcgtcactaa cctcttcatg ttcgccatct cgctcatcgc gctgttcggc tatctggctc
      421 cggcttatgc gcggccgccg gagtatgccg ccggggcgac gctgcccaat gccaccgacg
      481 ttcgcttcga ttcgttcgtc gccctgcgcg gctatgccgt cgataccgac gcggcgcggc
      541 cgggcgagcc gattggcatt gagttgtatt gggaagtgct ggccacgccg ccgggcgact
      601 acctgctgtt cgtccacctg accgacgacg agacgggcgc attggtggcc cagcgcgaca
      661 cccaccccgg tctgggcaac ttccccagca gtcagtggcg accgggcgac cgctttgtcg
      721 agcggatgaa aatgtacctg cctgagacgg cctacgcgcc ctcggccggg gaactgacca
      781 tcggcctcta cgcgcctgag ggctatcggc tgggcgtcac cgacaacgcg acgggcgaag
      841 ggttgggcga cgcctacccc atcggccgcg tcaccatcaa ctctcaagcc gcaccggatg
      901 gtgaaggatt gcccaatgcc ggggactaca actttgagga tcgcatccgc ctgctgggct
      961 acgattatga ccggcgcgtg ctgggcgtcg gtgagccgat cggcctgacg ccctactggg
     1021 aagcgctgcg gccgtcgccg ggcaattacg aggtcgagtt ggccgtgctg gacaatgata
     1081 ataatgtcgt cttcagtcaa accgacccgc tcaactcggc cgattggcag ccgggcgagg
     1141 tagtggttgg ctcatatacg ctgccgacag ggacaacgac cggcgatctg cgggtgcgcc
     1201 tggtgctgct cgacaccacc atcggccggc gcgtgaacag ggtcggcgac gacggccgct
     1261 ggatcgacga cgagctgttt ctgtcgaccg tggcggttcg cgattaggcc ggaaaggata
     1321 gcaggcagtg agcctggaat gccccgtaat tgccaccggg atcgtaggct aatcgttgcc
     1381 tggactgatt gacactcaat aggggttttg tatactaagt gtatggtgct gaaaataatt
     1441 acagcagctc ccaaacgaat acttctgtac acgcacgcct ggccgcgtgc gtttttgttt
     1501 ccggcagtag agggttgttc aagtagcaat agaggagata cacgacatgg attatggtat
     1561 gatcggcaag attgaaaagg cgaagttcta cgcggaagag cgagacactc gcattgtctt
     1621 tcaatcactt catgtgagca tcctgggcga caacgacaaa actcaccagg tgaagtacga
     1681 taatgggctt tggcactgcg attgcaacta cttcggctcg catcaggttt gtagccatac
     1741 catggcgatg gagcgcatcc tcggcagcat ggtcgagatg gggattcctt cttagtcgcc
     1801 acgttccgac aatctaatac aagataaagt gccctgtttc tgcgaaaaac agggcacttt
     1861 tcgttataat ccaacccata caaagcagga gcaatttgaa tggaatacgt aacacggccg
     1921 agcgaccatg ccggcgttcg ggccgacaag ttctataaga caaccctctt ccgcggggat
     1981 gcgctgatgc tgggcgtcaa ctgtctggag ccgggccaaa cgcagcccgc ccacgaccac
     2041 gccgatcagg acaagttcta ttacgtcatc gaaggagccg ggcgtttcca ggtgggaggc
     2101 gaattcatca cggccgggga agggcagctc gtctgggcgg cggcgggcgt cgtccacggc
     2161 gtgagcaatg agagcgatac gcgcctgacc ttgctggtcg gcatcgcgcc cgcgccatga
     2221 aagacggccg
//
LOCUS       DAOMQG010000483         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19456_length_2205_cov_9.171628, whole genome shotgun sequence.
ACCESSION   DAOMQG010000483 DAOMQG010000000
VERSION     DAOMQG010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19456_length_2205_cov_9.171628"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLC06_16290"
     CDS             complement(<1..410)
                     /locus_tag="PLC06_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRO92061.1"
                     /translation="MDLWHEFHGPNAGYILELYDRYRRDPQSVDPATRAAFEKWQPPD
                     ITNGHVAAIQANTPEADKVMGAVNLATTIREYGHLAARIDPLGMSNPPGDPGLHPNAH
                     GIDDADLAALPASIIGGPPATGAANAREAIRRLN"
     gene            complement(533..760)
                     /locus_tag="PLC06_16295"
     CDS             complement(533..760)
                     /locus_tag="PLC06_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92062.1"
                     /translation="MKTGMLWLDADARRTLDEKVERAVDYYRQKYGATPDLCLVNTTT
                     FTEEELKVGLVKVQRTRAVLPHHFWVGVSTA"
     gene            complement(798..2024)
                     /locus_tag="PLC06_16300"
     CDS             complement(798..2024)
                     /locus_tag="PLC06_16300"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRO92063.1"
                     /translation="METEPSSETRLRHQLDVQAQQINRVLSHHQVPATITGGTVRSRI
                     VSFDLQTQIAAGLERIMGLKSDLQSALGVGNVKITHAGDDWRLEVGRPDDPPVPLLKL
                     LGSLAPVPRSAIPVGLTEECTPIWLKFDGGRLGHILIAGDPGAGKTSLLRAIGVGLAL
                     TSRQSHLQLQILNPKLSDNGRVTVTDSPLLPLGYLPHMMADPSFGINECSALIHFLAE
                     EAAYRRRERLQLPRIVVLLDHIVTYLEEAEPSARKSLYQLLQYGAQAGIHLVMATDRA
                     ESTLLDSTLKASLSMRLIGRVSNPLVARRVAGAPLEQAPLLHGEGDFLAVIGEDVTYF
                     QAAYIGDYDLHLVLTQMARADRPALLAQPFTYRPRVKTGKKSTTQRAFTLHEEGVAFS
                     TQGVSNNDDPDMNIPF"
BASE COUNT          477 a          580 c          649 g          499 t
ORIGIN      
        1 tcgttcaatc gccggattgc ttcacgagca ttggccgcgc ccgtcgccgg tgggccgcct
       61 ataatggatg cgggcaaggc agctaaatcg gcgtcgtcga tgccgtgggc attgggatga
      121 agcccggggt ctcccggcgg gttggacata ccaagtgggt cgatcctggc cgccaaatga
      181 ccgtattcgc ggatagtcgt ggccagattg accgctccca ttaccttgtc tgcttcgggt
      241 gtattcgcct ggatggcggc gacgtgccca ttcgtgatat caggtggctg ccatttttca
      301 aatgcggcgc gggtggcggg gtcgacgctt tgggggtctc gccgataccg atcgtaaagc
      361 tcgaggatat agcccgcatt ggggccgtga aactcatgcc ataagtccat gggggagata
      421 ttaacaggtt ttgcgaggag gatcacccaa taggcggggt aatagctttc aacaaaaaag
      481 ccacctgtga cggtggctta aacgagcaga tgttcagggt cataagttta tgctaagcgg
      541 tgcttacgcc aacccaaaag tgatggggca acaccgcgcg cgtccgttga accttgacca
      601 gcccgacctt tagctcttcc tcagtaaatg tggttgtgtt gaccaggcat agatcaggcg
      661 ttgcgccata tttctgccgg taatagtcaa cggcacgttc cactttttcg tccagggtcc
      721 ggcgcgcatc cgcatcgagc caaagcattc cggttttcat gatgcacctc tctctacaaa
      781 atagctgtcc cttattccta aaaggggatg ttcatatccg ggtcatcgtt gtttgatact
      841 ccttgcgtgg aaaaagcgac cccttcttca tgcaaggtaa acgcccgttg agttgttgat
      901 ttcttgccgg tcttcactcg tgggcgataa gtaaatggct gcgccaaaag ggcaggccgg
      961 tcagccctgg ccatttgagt cagcacaagg tggagatcat aatcgccgat ataggccgcc
     1021 tgaaaatagg tgacatcctc gccgatgacg gccaggaagt cgccctcacc atggagtagg
     1081 ggggcctgtt cgagaggagc gccggctact cgacgggcga cgagtgggtt gctcacgcgg
     1141 ccgatcaatc gcatcgagag gctggccttg agcgttgaat ccaggagcgt tgattccgct
     1201 cgatcggtag ccatgaccag atggataccg gcttgcgctc catattgcag gagctgatag
     1261 agggactttc gggcgcttgg ttctgcttct tcgagatagg tgacgatatg atccagcagg
     1321 accacgatgc gaggcaactg caaacgctct cgccgacgat aggccgcctc ttcggccagg
     1381 aaatggatca gtgccgaaca ttcgttgatg ccgaatgatg ggtcggccat catatgaggc
     1441 aagtaaccca atgggagcaa tggggaatcc gtcacggtca cccggccatt gtcggataat
     1501 tttgggttca ggatttgcaa ttgcagatgt gattgccgac tggtcagggc aaggccgacg
     1561 ccaatcgccc taaggagcga ggttttaccc gccccgggat cgccggcgat caggatgtga
     1621 cccaatcggc cgccgtcaaa tttcagccag ataggggtgc attcctcggt taggccgaca
     1681 ggaatggccg atcgcggcac gggcgccaga gagccgagga gcttcagcag agggacgggg
     1741 ggatcatcag ggcgtcctac ttccagccgc caatcgtcgc cggcatgggt gatcttcacg
     1801 ttgcccacgc caagcgcgga ctgaagatcg ctcttcaagc ccatgattcg ttccagcccg
     1861 gcggcaattt gtgtttgcag atcaaaactg acgatacgcg agcgcactgt gccaccggtt
     1921 attgtcgccg ggacctgatg atggctcaga acccggttaa tttgctgggc ctgtacatcg
     1981 agttgatggc gcaatctggt ttccgaggat ggttcagttt ccattaccat ttcttgtttc
     2041 tactgttgag aacaattgtt ctaattaaat accaaaaaaa gggtggcctc gcgaggccac
     2101 cccggaaact atcgtcattc gattaaggtg cctgccgcaa gaccattaac acacggcctt
     2161 gaatttgaac ctcagcagga tcgacgtaga taggctccat cgtcg
//
LOCUS       DAOMQG010000484         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19457_length_2205_cov_6.797209, whole genome shotgun sequence.
ACCESSION   DAOMQG010000484 DAOMQG010000000
VERSION     DAOMQG010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19457_length_2205_cov_6.797209"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PLC06_16305"
     CDS             complement(<1..1337)
                     /locus_tag="PLC06_16305"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HRO92064.1"
                     /translation="MRRTDYMGLGLLLLAYVGLGAAFAVRTPVWQAPDEPAHYNYVRQ
                     LADGNWPVIEPGDWDQAYLSEVVGSEFDPAYPVVGITYEDWQPPLYYLAQWPVYQLSG
                     GSLVAMRLLSVLFGAGVVALAWVAGRAVFDGNAPRALAVAAFVAFLPQHLAILGSVNN
                     DGLAELIIAAILALLVLPWPDRPPRRRLLALGLLLGAGFLTKATAYLMAPVIGVFLLV
                     RYWPVKPDRSAVEQPVGGRISSADLSGLVRALLLVFVPALLLGLLWWGRNVSVYGGLD
                     VMAKGAHDAVVVGQPRTAEWITQYGLGGTIGRFLRTTFTSFWGQLGWMAAPLPGWMVG
                     PLVVGTVAAVVGLAVVSGQWSVVSRRKRLQALVLGLTFLLTLALLVFYNLTFVQHQGR
                     YLFPALIPIAVGFTAGLGYWLRPLERRWSWVAWLLPLGFAAVLGGMAVYALWRV"
     gene            complement(1452..>2205)
                     /locus_tag="PLC06_16310"
     CDS             complement(1452..>2205)
                     /locus_tag="PLC06_16310"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRO92065.1"
                     /translation="AAHLRLLAEVMAATTRARLEWDEMLETLPEDEATRRFLDETQIA
                     RYREELRDTLAGRRPSALVAERPGPSNTSHLSVIDADGLAVGLTTTAGESAGYIVPGT
                     GYIPNNMLGEEDLHPKGFHSRPAGQRIPTMMTPVIVLAGGRPRLVVGSGGSIRIRSAI
                     LQTLSNLLDYGLGLDAAVNLSRAHLDGDLLHLEAGYDPRAADELEAMGYRVNRWSARS
                     IYFGGAHSVARDADGRLHAAGDNRRGGATGEA"
BASE COUNT          409 a          807 c          689 g          300 t
ORIGIN      
        1 acccgccaca acgcatacac cgccatcccc cccagcacgg ccgcgaaccc cagcggcagc
       61 agccaggcca cccacgacca ccgccgctcc agcggccgca gccaataccc cagcccggcc
      121 gtgaacccca ccgcaatcgg gatgagcgcc gggaacagat agcgcccctg atgctgcacg
      181 aacgtcaggt tatagaagac gaggagggcg agggtgagga ggaaggtcag gccaaggacg
      241 agggcttgca gacgtttccg gcgactgacc actgaccact gaccactgac cactgccagc
      301 cccaccaccg ctgccaccgt ccccaccacc agcggcccaa ccatccagcc cggcagcggc
      361 gcggccatcc agccgagctg gccccagaag ctggtgaacg tcgtgcgcag gaagcggccg
      421 atcgtgccgc ccaatccgta ctgcgtgatc cattcggccg tgcgcggctg gccgacgacg
      481 accgcgtcgt gggcgccctt ggccatcacg tccagcccgc catagacgct cacgttgcgc
      541 ccccaccaca gcaggcccag cagcagtgcc gggacgaaga cgagcagcag ggcgcggact
      601 aaacctgaca ggtcggcgga tgagattctt ccaccaaccg gttgttcgac cgccgacctg
      661 tcaggtttca ccggccaata ccgcaccagc agaaacacgc caatcaccgg ggccatcagg
      721 taggccgtcg ccttggtcag gaagcccgcg cccagcagca gacccagcgc cagcaggcgg
      781 cggcgcggcg gccgatcggg ccagggcagc accagcaggg ccagaatcgc ggcgatgatc
      841 agctcggcca gaccgtcatt gttgaccgag ccgaggatgg ccagatgttg cggcagaaag
      901 gcgacgaagg cggccacggc cagcgcgcgc ggcgcgttcc cgtcgaacac ggcgcggccg
      961 gcgacccagg ccagcgccac cacccccgcg ccgaacagca ccgacaacag ccgcatggcg
     1021 accaacgagc cgccactcaa ttggtagacg ggccattggg ccaagtaata gagcggcggc
     1081 tgccagtcct cgtaggtgat acccactacc gggtaggccg ggtcgaactc cgagccgacg
     1141 acttcgctca ggtaggcctg atcccagtcg cccggctcga tgaccggcca gttgccgtcc
     1201 gccagctgcc gcacgtagtt gtagtgggcc ggctcgtcgg gggcctgcca gaccggcgtg
     1261 cgcacggcga acgccgcgcc cagccccaca taggcgagaa gtaataatcc cagccccatg
     1321 taatccgttc gtcgcatacc ctatacctgg aaaccgagtt ttttcctgaa aactcggttt
     1381 ctaaaaattt acgcctgata cattaaacgt taaaaaccgg gtttccggga agaaacccgg
     1441 ttttgagctt atcatgcctc gcccgtggcc ccgccgcgcc ggttgtcccc cgccgcgtgg
     1501 agccgtccgt cggcgtcgcg ggccacgctg tgcgctccgc cgaagtagat actgcgcgcc
     1561 gaccagcgat tgacgcgata gcccatcgct tccagctcgt cggccgcgcg cggatcataa
     1621 cccgcctcca gatggagcag gtcgccgtcg agatgggcgc gggacaggtt caccgccgcg
     1681 tccagcccca gcccatagtc gagcaggttg ctcagcgtct gcaggatggc gctgcggatg
     1741 cggatgctgc cgccgctgcc gaccaccagc cgcggccgcc caccggccag cacgatcacc
     1801 ggcgtcatca tcgtcgggat gcgctgcccc gccgggcgac tgtggaaccc cttggggtgc
     1861 aaatcttcct cgccgagcat gttgttggga atgtagccgg tgccgggcac gatgtagccc
     1921 gccgactcgc cggcggtcgt ggtcagcccc acggccagcc cgtcggcgtc gatgacgctc
     1981 agatgactgg tgttcgacgg accgggccgc tcggcgacga gagccgacgg ccgtcgcccg
     2041 gccagcgtgt cgcgcagttc ctcgcggtag cgcgcgatct gggtctcgtc cagaaagcgg
     2101 cgcgtggcct cgtcctccgg cagcgtctcc agcatctcat cccactccag ccgcgcccgc
     2161 gtcgtggcgg ccatcacctc ggccagcaac cgcaagtggg cggcc
//
LOCUS       DAOMQG010000485         2198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19532_length_2198_cov_4.238917, whole genome shotgun sequence.
ACCESSION   DAOMQG010000485 DAOMQG010000000
VERSION     DAOMQG010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19532_length_2198_cov_4.238917"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLC06_16315"
     CDS             <1..458
                     /locus_tag="PLC06_16315"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRO92066.1"
                     /translation="GSVLRHFEEEMAAVVSLAFFIPLLIGTGGNAGSQTTSTIIRALA
                     VEDLDKRGLLRPLLHETMIGLVLGTIMAVVAYGRALLWHTGEAVALTVAVAIFAIVVW
                     ANILGSVLPIVANRLKIDPTVVSGPAMSTLVDATGLFIYFTIAGLILGL"
     gene            complement(490..>692)
                     /locus_tag="PLC06_16320"
     CDS             complement(490..>692)
                     /locus_tag="PLC06_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92067.1"
                     /translation="PTPTVTPTGGGTPTPTATPTGGATTTPTATLPPVTPNAWNYLPA
                     LLGSRPTPVSTPVPTPVPTPAP"
     assembly_gap    693..724
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<725..2111)
                     /locus_tag="PLC06_16325"
     CDS             complement(<725..2111)
                     /locus_tag="PLC06_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRO92068.1"
                     /translation="MIDDANPSKERARPSMVVRALFVTLLVAGVLLSALIAGRDATAD
                     AGPAVGPAAALSSRPNFVVVMTDDQDIDSLPVMRHLLSYVGGGWVRFTSAFANDAKCC
                     PSRASFLTGQYSHHHGVVGNGKGDQLDDDHTLPVWLNDVGYHTGLFGKYLIGYPWALP
                     GSYQVPGWDVFMENHRPVDNQTDQALAFLDQAPADAPFFLYLAYTAPHHEAQPPERYR
                     NEDVYLPPRRPNFNEVDVSDKPLWVRRLPLLSNALIGKWDKERANSQRELLAIDDGVL
                     AIINKLQAMGRMDDTIFIFVADQGFSWGSHRWLYKNCPYVECSSFPLFIRVPGGANRV
                     ETRLVSNVDLAPTIAELAGAPITGRVPDGRSFAALLTDPGVAWAEGVLMERHEGDPPS
                     LFYAAQSNHYMYSEYGNGDKELYDMIADPYQMENVVDDPAHAAARAEMAALLAGLLAG
                     EPLPTPTATPPG"
BASE COUNT          352 a          696 c          754 g          364 t
ORIGIN      
        1 ccggcagtgt gctgcgccat ttcgaggaag agatggccgc cgtggtgtcg ctggctttct
       61 tcatcccgct gctcatcggc acgggcggca acgccggctc gcaaaccacc agcaccatca
      121 tccgcgccct ggccgtggaa gacctggaca agcgcgggct gctgcggccg ctgcttcacg
      181 agacgatgat cggcctggtg ctgggcacaa tcatggccgt ggtcgcctat ggccgggcgc
      241 tgctgtggca caccggcgag gccgtcgccc tgacggtggc cgtggccatc ttcgccatcg
      301 tcgtctgggc caacatcctc ggctcggtgc tgcccatcgt ggccaatcgg ctgaagatcg
      361 accccaccgt cgtctccggc ccggcgatga gcacgctggt ggacgcgaca gggctgttca
      421 tctatttcac catcgccggg ctgattctgg gcctgtgagc gcgaggcggc cggctcgcct
      481 ggccgtcgat cacggcgcgg gtgtgggcac gggcgtgggc acgggcgtgg acacgggcgt
      541 gggacggctg cccaacagcg cgggcagata gttccacgca ttgggggtga cgggcggcag
      601 cgtggccgtg ggcgtagttg tggctccgcc ggtgggcgtg gctgtgggag tgggcgtgcc
      661 cccgccggtg ggcgtgactg tcggcgtggg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnncgccgg gaggcgtggc cgtgggcgtc ggcagcggct cgcccgccag cagcccggcc
      781 agcagcgccg ccatctcggc ccgcgccgcc gcatgggccg ggtcgtcgac cacattctcc
      841 atctgatacg gatcggcgat catgtcataa agctctttgt cgccgttgcc atattcgctg
      901 tacatatagt ggttggactg cgcggcatag aacagggacg gcggatcgcc ctcatgccgt
      961 tccatcagca ccccttcggc ccaggcgacg cccggatcag tcagcagcgc ggcgaagctg
     1021 cggccgtcgg gcacgcggcc ggtgatgggc gcgccggcca gctcggcgat ggtcggggcc
     1081 aggtcgacat tcgacaccag ccgcgtttcc acccgattcg ccccgccggg cacgcggatg
     1141 aacagcggga agctgctgca ttccacatag gggcagttct tgtagagcca gcgatgcgac
     1201 ccccacgaga acccctggtc ggccacaaag atgaaaatcg tgtcgtccat ccggcccatc
     1261 gcctgcaact tgttgatgat ggccaggacg ccatcatcga tggccagcag ttcgcgctgg
     1321 ctgttggcgc gctccttgtc ccatttgccg atcagcgcgt tggacagcag cggcaagcgc
     1381 cgcacccaca gcggcttgtc gctgacatcg acctcgttga agttcggccg ccgcgggggc
     1441 aaatagacat cctcgttccg gtagcgctcg ggcggctggg cctcgtggtg gggtgcagtg
     1501 taggccagat agaggaagaa cggcgcgtcg gccggggcct ggtcgaggaa ggccaacgcc
     1561 tggtcggtct gattgtcgac cggccggtgg ttctccatga acacatccca ccccggaacc
     1621 tgatagctgc cgggcaatgc ccagggatag ccgatgaggt atttgccgaa gaggccggta
     1681 tggtagccca catcattgag ccacacgggc agggtgtggt cgtcgtccag ctggtcgccc
     1741 ttgccgttgc cgacgacgcc gtggtggtgg ctgtactggc cggtgaggaa cgaggcccgc
     1801 gacgggcagc acttggcgtc gttggcgaag gccgacgtga agcgcaccca ccccccgccg
     1861 acataggaca gcagatggcg catgaccggc aggctgtcga tgtcctgatc gtcggtcatg
     1921 acgacgacga aattgggccg cgacgacagg gcggccgccg ggccaactgc cgggccggca
     1981 tcggcggtcg cgtcgcgccc ggcaatcagc gccgacaaca gaacgccggc gacgagcagc
     2041 gtcacaaaaa gtgccctgac caccatcgac ggccgggcgc gctccttcga cgggttggca
     2101 tcgtcgatca tcgagcgact ccagcgaccg tcggggaaaa gcgaaggaaa ccaaagattt
     2161 caaaacgcat tggtgacttc gcctcacgct tatttttt
//
LOCUS       DAOMQG010000486         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19574_length_2194_cov_9.697990, whole genome shotgun sequence.
ACCESSION   DAOMQG010000486 DAOMQG010000000
VERSION     DAOMQG010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19574_length_2194_cov_9.697990"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2107)
                     /gene="uvrA"
                     /locus_tag="PLC06_16330"
     CDS             complement(<1..2107)
                     /gene="uvrA"
                     /locus_tag="PLC06_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRO92069.1"
                     /translation="MSLDKIIVRGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMEKPDVDGIEGLSPAVSIDQRGVSHNPRSTVGT
                     VTEIYDYLRLLFARVGTPHCPQCGRPVMPQSAEQIVSNVMAMPIGTRIQVLAPLVQDR
                     KGHHQGIFDDVRKAGFVRVRVNGEVRQVDDKIELDRYKNHSIEAVVDRLIIRHPANDE
                     SEEAASDRSRLTDSIETALRLGSGIAIVNDVTNPDAPVDNLYSEHLYCPYDGTSIPPI
                     EPRTFSFNTPYGACPDCQGLGFQKEIDPDLVVPNKDLALRGGAIIGWPTEDKAGYYWQ
                     LLKATAENFSIPLDLPVRELSPQQMRILLYGSGDESVTVTYRNREGHTRHYGTKYEGV
                     IPNLWRRYRESTSDYVREKLEEYMVDSPCRACGGSRLQPVARAVDIGGGTIVEAVRRP
                     VVKTLEWTQCLEGRETTPLTPRQQTIAKPILKEIRERLSFMINVGLDYLTLDRTAGTL
                     SGGEAQRIRLATQIGSQLMGVLYVLDEPSIGLHQRDNARLIETLRGMRDLGNTVLVVE
                     HDEETMRAADWLLDLGPGAGKLGGELIAEGTPDQVAKNRKSLTGAYLSGRKSIPVPGK
                     RRPGNGEWLEVIGAAENNLKDVDIRIPLGKFVCITGVSGSGKSSFLVEILSKRLNQHF
                     YGSKALPGKHQEIRGLEHLD"
BASE COUNT          353 a          680 c          726 g          435 t
ORIGIN      
        1 tgtcgagatg ctccagcccg cgaatctcct gatgcttgcc gggcagggcc ttcgaaccgt
       61 agaaatgctg gttgagccgt ttgctgagga tctcgaccag gaaggagctt ttgccgctgc
      121 cgctcacgcc ggtgatgcag acgaacttgc ccagcgggat gcggatatcg acatccttca
      181 ggttattctc ggccgcgccg atgacttcca gccactcacc attgccgggg cggcgctttc
      241 cgggcacggg gatcgattta cggccgctca ggtaagcgcc ggtgaggctt ttcctgttct
      301 tcgccacctg atctggtgtg ccctcggcaa tcagctcgcc gccgagcttg ccggctcccg
      361 gccccaggtc gagcagccag tcggcggcgc gcatcgtctc ctcgtcgtgc tccaccacga
      421 ggacggtgtt gcccagatcg cgcatgccgc gcaaggtctc gatgaggcga gcgttgtcgc
      481 gctggtgcag gccgatgctc ggctcgtcaa gcacatagag cacgcccatg agctggctgc
      541 cgatctgcgt cgccagccgg atgcgctgcg cctcgccgcc gctgagtgtg cccgccgtgc
      601 ggtcgagggt caggtagtcg aggccgacat tgatcatgaa gctcagccgc tcgcgaattt
      661 ccttcaggat cggcttggcg atcgtctgct ggcggggcgt cagtggggtg gtctcccggc
      721 cctcaaggca ttgagtccat tccagcgtct tgaccaccgg ccggcgcacc gcctcaacga
      781 tagtcccgcc gccgatatcg acggcgcggg ccaccggctg caggcgcgag ccgccacagg
      841 cgcgacacgg cgaatcgacc atgtactctt ccagcttctc gcgcacgtag tcggaagtcg
      901 actcccggta gcgccgccag aggttgggga tgacgccttc gtactttgtg ccgtagtggc
      961 gcgtgtgccc ctcgcggttg cgatatgtga ccgtgacgga ttcgtcgccg ctgccgtaga
     1021 gcaggatgcg catttgctgc ggtgacagct cacgcacagg cagatcgagc ggaatggaga
     1081 agttctcggc cgtggctttc aacagttgcc agtaatagcc ggccttgtct tcggtcggcc
     1141 agccgatgat agcgccgccg cgcaatgcca agtccttgtt gggcacgacc agatcgggat
     1201 cgatctcctt ctgaaagccc aggccctggc aatcgggaca cgcgccatag ggagtgttga
     1261 agctgaacgt gcgcggctcg atgggcggga tactcgtccc gtcatagggg cagtagaggt
     1321 gctcggaata gagattgtcg acgggcgcgt cggggttcgt cacatcattg acgatggcga
     1381 tgccgctgcc caatcgcaac gccgtctcga tcgagtcggt cagccggctg cgatcggacg
     1441 cggcttcctc gctctcgtca ttggccgggt ggcggatgat cagtcggtcg accacggctt
     1501 cgatggagtg gttcttgtag cggtcaagct cgatcttgtc gtctacctgg cgcacttcgc
     1561 cattgacgcg cacacgcacg aagcccgcct tgcgcacatc gtcgaagatg ccctgatggt
     1621 gccccttgcg atcctgtacc agcggggcca gcacctgaat gcgcgtgccg atgggcatcg
     1681 ccatgacgtt actgacgatc tgctcggccg actggggcat gacggggcgg ccgcattgcg
     1741 ggcagtgggg cgtgccgacg cgggcgaaga gcaagcgcag gtagtcgtag atttcggtca
     1801 ccgtgcccac ggtggagcgc gggttgtggc tgacgccgcg ctgatcgatg gagacggccg
     1861 ggctgagtcc ctcgatgccg tcgacgtcgg gcttctccat ctggccgagg aactgccggg
     1921 cgtaggccga gagcgactcg acgtagcggc gctgcccctc ggcgaagatg gtgtcgaagg
     1981 ccagcgacga cttgcccgaa cccgacaagc cggtgatgac caccagcttg tcgcgcggga
     2041 tctccacgtc gatattcttc aggttgtgtt cgcgcgcgcc gcgcacgatg atcttgtcga
     2101 gtgacatgtt cttttttctt tttgcgcgca gggacggcat ggagacctcc gcgcggccga
     2161 tagagccaac ctgctattct agcatggcaa tcgg
//
LOCUS       DAOMQG010000487         2183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19702_length_2183_cov_6.195019, whole genome shotgun sequence.
ACCESSION   DAOMQG010000487 DAOMQG010000000
VERSION     DAOMQG010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19702_length_2183_cov_6.195019"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLC06_16335"
     CDS             <1..261
                     /locus_tag="PLC06_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRO92070.1"
                     /translation="VDRAWMGVMKTPTGPFGIMDAVGLDTVWKITDFWAGVSGDPRSR
                     AGADFVKGYLERGRVGQKGGAGFYDYPDPAFRRPGFIDGETS"
     gene            258..593
                     /locus_tag="PLC06_16340"
     CDS             258..593
                     /locus_tag="PLC06_16340"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRO92071.1"
                     /translation="MMTLLLMYVVFGLLLIALAIPMMRDKVPPNAWYGFRVPSTRSNP
                     TVWYKANRYAGRWLLVTGIVTVAAAIVFYFVPGLSVDHYAWLVLAAFGVPFVLMIAAS
                     FRYLRGLPK"
     gene            complement(675..1850)
                     /locus_tag="PLC06_16345"
     CDS             complement(675..1850)
                     /locus_tag="PLC06_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO92072.1"
                     /translation="MSHTSYLIIGGGMTGAAAAVGIRELDSDGTITMISAETAPPYDR
                     PPLSKKLWSGKRTLEQIVAPLPADVELHLDRRVVAIDPDNRQATDDAGVVYTYDKLLL
                     ATGGAPRHLPFGGDAINYYRTVEDYRTLRAEAGHGDSFVVIGGGFIGSEIAAALRSQG
                     NDVTIIFPEDGIGNRLFPPGMPATINEMYRKHGVTVLSDASVVGVEKEGDRTVVITGD
                     GQRLSAGRVVAGIGIIPSVELAKAMGLAVDNGVTVDEYLRTARPEIYAAGDVANYPDL
                     ILGIHRRVEHADAARAMGRAAGRNMAGANEPYRYLPLFYSDLFELGYEAVGQCDPRLE
                     MVEDWQDVYSRGVVYYLKDGRVCGVLLVDVWDKVDEARALIEKGEKVDPKELINRIN"
BASE COUNT          382 a          660 c          716 g          425 t
ORIGIN      
        1 gtggatcgcg cctggatggg ggtcatgaag acgcccaccg ggccattcgg gatcatggac
       61 gccgtcgggc tggacacggt gtggaagatc accgacttct gggccggggt cagcggcgat
      121 ccgcggtcgc gggccggggc cgacttcgta aagggctatc tggagcgcgg ccgcgtggga
      181 caaaaggggg gcgcgggctt ctatgactat cccgatcctg ccttccggcg gccgggcttc
      241 atcgacggag aaacatcatg atgacattgc tgctgatgta tgttgtgttc ggcctgttgc
      301 tgatcgcgct ggcgatcccg atgatgcgcg acaaggtgcc gcccaacgca tggtacgggt
      361 ttcgcgtgcc cagcacacgg tcgaacccga ccgtgtggta caaggccaac cgctacgcgg
      421 ggcgctggct gctggtgacg ggcatcgtga cggtggccgc cgccatcgtg ttctatttcg
      481 tgcccggcct gtcggtcgat cattacgcct ggttggtgct ggccgcgttt ggggtgccgt
      541 ttgtgttgat gatcgcggcc agctttcgtt atttgcgcgg gttgccgaag tagggcagtg
      601 gatcagtgat cagtggatca gtggatcagt gatcagtgga tcagtggatc agtgatcagt
      661 gggtcagtgg tgggctaatt gatgcggttg atcaactctt tggggtcgac tttctcccct
      721 ttttcgatga gcgcgcgcgc ctcatcgacc ttgtcccaca catcgaccag cagcacgcca
      781 cacacgcggc cgtccttgag atagtagacc acgccgcggc tgtagacatc ctgccagtcc
      841 tcgaccatct ccagtcgtgg atcgcactgg cccaccgcct cgtaacccag ctcgaacagg
      901 tcggaataga acagcggcag atagcgatac ggctcgttgg cgccggccat gttgcgcccg
      961 gcggcccggc ccatggcgcg cgcggcgtcg gcgtgctcta cccggcgatg gatgcccagg
     1021 atcaggtcgg ggtagttggc cacgtcaccc gcggcgtaga tctccggccg cgcggtgcgc
     1081 agatactcat cgacggtcac gccattgtcc acggccagcc ccatcgcctt ggccagctcg
     1141 acggacggga tgatgccgat gccggcgacc acgcggccgg cgctcagccg ttgcccgtcg
     1201 ccggtgatga cgacagtgcg atcgccctct ttttccacgc ccactacgct ggcgtcgctc
     1261 agcaccgtga ccccgtgctt gcgatacatc tcgttgatcg tggcgggcat tcccggcgga
     1321 aacaagcggt tgccgatgcc gtcttcgggg aagataatgg tcacgtcgtt gccctggctg
     1381 cgcagcgcgg cggcgatctc ggagccgatg aagccgccgc cgatgacgac gaacgaatcg
     1441 ccgtgcccag cctcggcccg caacgtccgg taatcctcga cggtgcgata gtaattgatc
     1501 gcgtcgccgc cgaagggcag gtgtcgcggc gcgccgccgg tggccagcag cagtttgtcg
     1561 taagtgtaga ccacgcccgc gtcatcggtg gcctgccgat tgtcggggtc aatggcgacc
     1621 acgcgccggt cgaggtgaag ctcgacgtcg gcgggcagcg gggccacgat ctgctccagc
     1681 gtgcgcttgc ccgaccagag ctttttgctg agcggcgggc ggtcataggg cggcgcggtt
     1741 tcggccgaga tcatcgtgat cgtgccgtcg ctgtccagtt cgcgaatgcc gacggccgcg
     1801 gccgcgccgg tcatcccgcc gccgataatg agataggatg tatgggacat atagggaacc
     1861 tcccgttccg atgtaataat agtagagtat agcaagattg aggagagaag ggcgaggtac
     1921 gaagtacgaa gtacgaaaga ggcgctcttc attcgttgcc gtagacgacg gacgatagac
     1981 cacagaccac ggacgacggg ccacggacgt cattcgtcac tcgtcattcg tcactcttct
     2041 tgcccaaaac caatcatcgg ataaagtacc gcctaatgtc gccggcggcc gcggccggca
     2101 agtgtgatcc atcatttacg agtaaaaggt atccatggca tcaacccgcc cctatcaacg
     2161 attgctctac ggcgtcggct cca
//
LOCUS       DAOMQG010000488         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19870_length_2167_cov_12.060133, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000488 DAOMQG010000000
VERSION     DAOMQG010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19870_length_2167_cov_12.060133"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLC06_16350"
     CDS             <1..249
                     /locus_tag="PLC06_16350"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92073.1"
                     /translation="EMRLDFMLKLYFAMWEEPSIITELLRRQQMVCADWLASQQKKDV
                     AGSTYLAFVRRYRYGHIESIQNWLMMLANEFAGHQTPQ"
     gene            268..1119
                     /gene="modA"
                     /locus_tag="PLC06_16355"
     CDS             268..1119
                     /gene="modA"
                     /locus_tag="PLC06_16355"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRO92074.1"
                     /translation="MFKSWIEKQKTLTFAVLALLLLVASCTQNGGTAPTAPEQSPVEE
                     RTTLTVYAAASLTDAFGKLAETYKVNNSNVDVVFSFAGSNQLAAQINQGAPVDVFASA
                     NLSQMENVVAEGRIDADAPRVFVTNRLVLAYPEANPAGITTLADLALPGTLIVLAAEE
                     VPVGRYSLEFLDKATADPTFGSSFKEDVLANVVSYEENVRAVLNKVSLGEADAGIVYL
                     SDLVGVEGVSYLEIPDSLNSVAEYPIAALNDSKNAEAAAAFINLVIGAEGQAILGEYG
                     FGPAPAP"
     gene            1116..1955
                     /locus_tag="PLC06_16360"
     CDS             1116..1955
                     /locus_tag="PLC06_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO92075.1"
                     /translation="MRSFLTGDDKSPRRVVQRQRHRPETWWAFSLPLLLFLLIPLLAL
                     IGYSSPEQLVENLALPEVRQAIMLSLRTSTLATLASVLLGTPMAYLLARREFPGRLLL
                     DNLIDLPIVLPPAVAGLSLLLVFGRRGLVGVWLDSFGLRVTFTETAVVIAQTFIAVSL
                     FIRAASIGFAGVEPELEDAAAIDGASHWQTFSRITVPLARRAVLTGIALAWARALGEF
                     GATIIFAGNYPGRTQTMPLAIYLGFELNLDVAVTLSVILVLLSFIVLLVVKGFFGIGD
                     QEE"
BASE COUNT          431 a          587 c          624 g          525 t
ORIGIN      
        1 gaaatgcgac tggacttcat gctgaagttg tatttcgcga tgtgggaaga accctcgatc
       61 atcaccgaat tacttcggcg acaacagatg gtctgcgctg actggctggc aagccaacaa
      121 aagaaagacg tcgccggaag cacctacctg gcctttgtgc gtcgatatcg ttatggccac
      181 atagagagta tccaaaactg gttgatgatg ttggcaaacg agtttgccgg ccatcagacc
      241 ccccaatagt tcatcttaga ggaagaaatg ttcaaatcgt ggattgaaaa gcaaaagact
      301 ctgacctttg cggtgctggc cttgctgcta ttggttgcat cttgcacgca aaatgggggc
      361 acagccccaa cggcacctga acaatcccct gttgaggaaa ggaccacgct tactgtctat
      421 gcggccgcct ctcttaccga tgccttcggg aagcttgcag agacctataa ggtgaataac
      481 tccaatgttg atgtggtctt cagttttgcc gggtccaatc aactggcggc acagatcaat
      541 cagggcgctc cggtcgatgt gttcgccagt gccaatttgt cacaaatgga gaatgttgtc
      601 gccgagggga ggatcgatgc agatgcgccg cgcgtctttg ttacgaaccg tcttgtactg
      661 gcctatcccg aagcaaatcc ggccggcatc accaccctgg cagacctggc cttgcccggc
      721 acattgattg tcctggcggc tgaagaggtg ccggtaggcc gctattcgct ggagtttttg
      781 gataaggcaa cggccgaccc aacgtttggg agcagcttta aggaagacgt gctggccaat
      841 gtggtgtcct atgaggaaaa cgttcgcgcc gtattaaata aggtgtcgct gggcgaggct
      901 gatgcgggta ttgtctacct gagtgacctt gtgggggtgg agggcgtcag ctatctggag
      961 atacccgata gcctgaatag cgtggcggaa taccctatcg ccgccttgaa tgacagcaaa
     1021 aatgcagagg cggccgcggc attcatcaat ttagtgattg gagcggaagg gcaagccatc
     1081 ctcggcgagt acgggtttgg accggcccca gccccatgag gtcttttctc accggtgacg
     1141 acaagtcgcc acggagggta gtacagcgac aacgccatcg gccagagacg tggtgggcgt
     1201 tctccctgcc gctgctgctt ttccttctga tcccgctgct ggcgctcatc ggctacagca
     1261 gcccggaaca actggtagag aacctggcct tgccggaagt ccggcaagcc atcatgctca
     1321 gcctaaggac cagcaccctg gctacgctgg cctccgttct tttgggcacg ccgatggctt
     1381 atctgctggc acggcgcgaa ttccccggcc ggctgctgct cgacaacctc attgatctgc
     1441 ctatcgtctt gccgccggct gtggcggggc tgtcgctgct gctcgttttt gggcgccggg
     1501 ggctggttgg cgtctggctc gattcgtttg ggttgcgggt aaccttcacc gagacggccg
     1561 ttgtcatcgc ccagaccttc atcgccgtct cgctcttcat tcgcgccgca tcaattggtt
     1621 tcgccggtgt tgagcctgaa ctggaggacg cggccgccat cgacggagcc agtcactggc
     1681 aaacattttc gcgtatcacc gtgccgctgg cccggcgagc ggtgctgacc ggcattgccc
     1741 tggcttgggc gcgtgccctg ggcgaatttg gcgcgacgat catcttcgcc ggcaactacc
     1801 ctggccgcac acaaacgatg ccgctggcga tctaccttgg cttcgagctt aatctggatg
     1861 tagctgtgac gttgtcggtc attctcgtgc ttttgtcgtt tatcgtcctg ttggtggtta
     1921 aaggcttctt cggtattggg gatcaggaag aataaacgcc ggaatgatcg atgcgattgt
     1981 ctctattgaa ttaggtgcat ctatcctctt ctgctggtat catcttcaag ggatcccaac
     2041 gatggtaagc gcttgacgcg tttaccctac cgattctttt catctatcct tgattgcaac
     2101 cttatcaccc ttatagcccc atagttccgg agggagtatg ctaggcttta ttcagatatt
     2161 gggcgga
//
LOCUS       DAOMQG010000489         2163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19922_length_2163_cov_11.419355, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000489 DAOMQG010000000
VERSION     DAOMQG010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19922_length_2163_cov_11.419355"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1051
                     /gene="rpsB"
                     /locus_tag="PLC06_16365"
     CDS             131..1051
                     /gene="rpsB"
                     /locus_tag="PLC06_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRO92076.1"
                     /translation="MAIVSMKALLETGVHFGHRTRRWNPKMKPYIFTERNGIHILDLQ
                     QTIVLIEEAYNAIRDTVAAGGDVLFVGTKRQAQDTVAREASRATQPYVNDRWLGGTLT
                     NWRTIRQRINYLRELETRRDAGEFDLLKKTERLRIEREIEKLNLRLGGIRDLRDLPAM
                     LFIVDVSHEVTAIREANTLNIPIIGVVDTNSDPDPIDYVIPSNDDAIRAIKLIVGKMA
                     DAALEGLAMRKDTLQEEVTDFDRYRYESFDGMEDAEDEMLLGASTLAKLREKAAIDEV
                     LADDDDTKEDVDVDIDEFLTEADEADEASE"
     gene            1075..1680
                     /gene="tsf"
                     /locus_tag="PLC06_16370"
     CDS             1075..1680
                     /gene="tsf"
                     /locus_tag="PLC06_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRO92077.1"
                     /translation="MAVTTEMVKELRAVTGAGVLEAKKALEQHNGDFDKAIDMLREKG
                     AARAAKRADRSAKEGVIELYAHPGNRVGVMLELNCETDFVARNEQFRDLAHNLALHIA
                     AAAPRYLNKEDVPTQELDRETSVLKAQALAEGKPEAVIEKVISGRIAKFYEEMCLMEQ
                     PFIKDEKIKIKEMMTDAISSTGENIIVRRFARYELGEALED"
     gene            1790..>2163
                     /gene="pyrH"
                     /locus_tag="PLC06_16375"
     CDS             1790..>2163
                     /gene="pyrH"
                     /locus_tag="PLC06_16375"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011668022.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRO92078.1"
                     /translation="MADVRYKRILLKMGGEALAGQGGFGIDPERANEVAQVVKDVHDL
                     GVEIALVIGAGNLWRGSVGTQAGMERSTADHIGMVATVMNALALKDVLERAGVVTRVQ
                     TAFEMRTIAEPYIRLRAIRHMD"
BASE COUNT          499 a          598 c          672 g          394 t
ORIGIN      
        1 cccgcgactc tcccaggtca ctgacccatg tgaccgcctc ggagcattcg ttggctgcct
       61 tggccatgca agttccgctt caggttgaag ggtgtggaag acgaaaacaa aattaaagga
      121 gaataacccc atggccattg tttcaatgaa agcacttctg gaaacaggcg tgcacttcgg
      181 tcaccggacg cgtcgctgga acccgaagat gaagccttac atctttaccg agcgcaacgg
      241 gatccatatc ctcgacttgc agcagacgat cgtgctgatc gaggaagcct ataacgccat
      301 ccgcgacacc gtcgccgccg gcggtgacgt gctcttcgtg ggcaccaagc gccaggcaca
      361 ggacaccgtc gcccgcgaag cctcgcgcgc gacgcaaccc tacgtcaatg atcgctggct
      421 cggcggcacg ctgaccaact ggcgcaccat ccgtcagcgc attaactatt tgcgcgaact
      481 ggagacccgt cgcgacgccg gtgaattcga cctgctgaag aagacggagc gtctgcgtat
      541 cgagcgtgag attgagaagc tcaacctgcg tttgggtggt atccgtgacc tgcgcgatct
      601 gccggccatg ttgtttatcg tcgacgtgag ccacgaagtg acggccattc gcgaggccaa
      661 taccctgaat attccgatta tcggcgtagt ggatacgaac agcgatcccg atccgatcga
      721 ttacgtcatc ccgtcgaacg acgatgccat tcgggcgatc aaactgatcg tcggcaagat
      781 ggccgatgcg gccctggaag gcctggccat gcgcaaggat acgttgcagg aagaagtgac
      841 cgacttcgac cgctatcgtt atgagtcatt cgacggcatg gaagacgccg aagacgagat
      901 gctgctgggc gcttccacat tggccaagct gcgcgagaag gctgccatcg atgaagtgct
      961 ggcagatgac gacgacacga aagaagatgt ggatgtcgat attgatgaat tcttgacgga
     1021 agccgatgaa gcagacgagg cgtccgagta aacgttaggg agataagatt cgagatggca
     1081 gttacgaccg aaatggttaa agagctacgc gcagtcaccg gcgcgggcgt cctggaagcc
     1141 aagaaggccc tggaacaaca caacggtgat ttcgacaagg cgatcgacat gctgcgcgag
     1201 aagggcgcgg cccgcgcagc caagcgggcc gaccgctcgg ccaaagaagg cgttatcgag
     1261 ctgtatgccc accccggcaa ccgcgtgggc gtgatgctgg aactgaattg cgagactgac
     1321 ttcgtcgctc gcaacgagca gttccgcgac ctggctcaca acctcgcgct tcatattgcc
     1381 gccgccgctc cgcgctatct gaataaggaa gacgttccca cccaggaatt ggatcgtgaa
     1441 acgtcggtgc tgaaggcgca ggcgctggcc gagggcaagc ccgaagctgt gattgagaag
     1501 gtgatctccg gccgcatcgc caagttttat gaggagatgt gcctgatgga gcaacccttc
     1561 atcaaggacg agaagatcaa gatcaaggaa atgatgacgg acgccatcag ctcgacaggc
     1621 gagaacatca tcgtccgccg ctttgcccgc tacgagctgg gtgaagcgct ggaagactaa
     1681 ttcctgtcgg ccaagtgaga cctgacgagg cggcgatatg gggcttatct gactgaagcc
     1741 tgcatcgccg cccgtcaggt ctcgataata taaaaaggaa cgacgaacga tggccgatgt
     1801 tcgatacaag cggattctgc tgaagatggg cggggaggcg ttggccggcc agggcggctt
     1861 tggcatcgat cccgaacgtg ccaacgaggt cgcccaggtg gtgaaggacg ttcatgattt
     1921 gggcgtcgag atcgcgctgg ttatcggcgc gggtaacttg tggcgcggct cagtgggcac
     1981 gcaagccggg atggagcggt caacggccga ccacatcggc atggtggcca cggtaatgaa
     2041 cgcgctggcg cttaaggacg tactggagcg cgccggcgtc gtcacccgcg ttcagaccgc
     2101 gtttgagatg cgcacgattg ccgagcctta catccggttg cgggccattc gccacatgga
     2161 caa
//
LOCUS       DAOMQG010000490         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19967_length_2159_cov_9.809886, whole genome shotgun sequence.
ACCESSION   DAOMQG010000490 DAOMQG010000000
VERSION     DAOMQG010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19967_length_2159_cov_9.809886"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLC06_16380"
     CDS             complement(<1..516)
                     /locus_tag="PLC06_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92079.1"
                     /translation="MRRWLFVIGGAIILLASTACGSRDNQPAPPSSENDAVAESVRET
                     VAAIAAQTAEASANQATPTTVSASGATAPSCTVLAGVNLRNGPGLAYDPPVAVADPGT
                     SLRPIAYVPSGFPLGAWLLVEAGDADQPLWVSAGPQFVSCTVDVATLPRPAAIPPTPV
                     PSSSPTPTISSP"
     gene            complement(574..1581)
                     /locus_tag="PLC06_16385"
     CDS             complement(574..1581)
                     /locus_tag="PLC06_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO92080.1"
                     /translation="MRSLFALAVLCVSVVALTVACGGGDEVTPPQTIDAVAQSVQQTV
                     EAIAVAQTVAALTGGTPVAETELPPVPTPQVTPVATTAATAAPPSPTVAQPSPEAAVS
                     CTTLTGVNLRNGPGLAYDPPVGSIGANTSLRPLAYVPLGHPQGAWLQVEVAGTGQVAW
                     VSAGAQFISCTADLSTLPPPVAVLPTPVKATATPQQVANLPPDLRNISGGAGCLDDPD
                     IDSDFQTDSRFLLRVFAQLFDPPSGESGDGAGIDRVEFSVRESGYTHTERTAGYCIFQ
                     GGEPACRDWPRNALGQLTWGNDGPVVVDGDYNIDVNVFPKEETFISQCNWTISLTIDV
                     P"
     gene            complement(1587..>2159)
                     /locus_tag="PLC06_16390"
     CDS             complement(1587..>2159)
                     /locus_tag="PLC06_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92081.1"
                     /translation="VHYLRAQTLLDDSQAEDFTGDRVAAVESAIAEARLALEAYKPVA
                     AGEEQVEVYGLPIASGANYEAGIALRLLADAYYRIGSVDEAKAAVDEAIARLEPAVGP
                     LVESGNSRLIAQTYQALGTAYEWRSFLLTQAGDQAAAGEANGRAIANYEACVAVREQF
                     PFDAFMLEQIIRDLCQPRLDVLKSGGTGDG"
BASE COUNT          423 a          714 c          652 g          370 t
ORIGIN      
        1 cggcgatgaa attgtcggag tcggtgacga ggacgggaca ggcgtcggcg ggatggccgc
       61 cgggcgagga agcgtcgcca catcaacggt gcaggagaca aactgcgggc cggcgctgac
      121 ccacagcggt tgatcggcat cgcctgcctc aaccagcaac cacgctccca acggaaagcc
      181 ggaaggcaca taggcgatcg gccgtaacga ggtgcccggg tccgccactg ccaccggtgg
      241 gtcataggcc aatcccggcc cattgcgcag gttaaccccg gcaagcaccg tgcaagacgg
      301 ggccgttgcg ccggaagccg atacggtggt cggcgtggcc tggttcgccg acgcttcggc
      361 cgtctgcgcc gcaatagccg ctactgtctc ccgaacggat tcggcgaccg catcattttc
      421 ggacgacggc ggcgcgggtt gattatcccg gctcccgcaa gccgtcgagg ccagcaaaat
      481 gatggccccg ccaattacga acagccacct tctcatttct cacccccgaa actccttgtc
      541 ctccatctga agctgcgaac actctccacg acgctacggc acgtcaattg tcagcgatat
      601 agtccagttg cattgcgaga tgaaagtctc ctccttcgga aacacgttaa cgtcgatgtt
      661 gtagtcaccg tctacgacga ccggcccgtc gttaccccag gtaagttggc cgagcgcgtt
      721 gcgcggccaa tcgcggcagg ccggctcacc cccctggaag atgcagtacc cggcggttcg
      781 ctccgtgtgg gtatagccgc tctcgcggac ggagaactcc acccgatcga tgcccgcgcc
      841 gtcgcccgat tcacccgatg gcgggtcaaa cagttgggca aaaacccgca gcaggaatcg
      901 tgaatcggtc tgaaaatcgg aatcgatatc gggatcatcc aggcaaccgg cccccccgga
      961 aatattgcgc aggtcgggtg gcaggttggc cacttgttgt ggggtagccg tcgccttgac
     1021 cggggtcggc aggacagcga ccggcggcgg cagggtggac aagtcggctg tacaacttat
     1081 gaactgcgcc ccggcactga cccaggctac ctgccccgtg ccggcaacct cgacctgtag
     1141 ccaagctccc tgggggtgtc ccagcgggac gtaagccagc ggccgcagcg aggtattggc
     1201 tcctattgat ccaaccggcg gatcataggc caggcccggc ccgtttcgca gattgacacc
     1261 ggtgagagtc gtacatgaga cggccgcttc gggcgagggt tgggcgaccg tcgggctggg
     1321 cggggcagcg gttgccgccg tcgtagcgac aggagtcacc tgtggtgtag ggacaggcgg
     1381 cagctcggtt tcagcgaccg gtgttccgcc ggtcagcgcc gcgaccgtct gagctacggc
     1441 gatcgcctcc actgtctgct gcacggattg agccacagca tctatcgtct gaggcggggt
     1501 aacctcatca cctccgccac aagcgaccgt taacgcaaca accgaaacac acaggacagc
     1561 cagagcaaat agacttctca tgttcatcac ccatctcctg tcccccccga tttcaacaca
     1621 tcaaggcgcg gttgacacag gtcccggatg atctgctcca acataaaggc gtcaaacggg
     1681 aactgttccc gcacggcgac acacgcctca taattggcga tcgccctgcc gttcgcttcg
     1741 ccggccgccg cctgatcccc ggcttgggtc agcagaaagc tgcgccactc ataggccgtg
     1801 cccaacgcct gataggtctg ggcgatcagg cggctgttcc cggattcaac cagcggcccc
     1861 acagccggct ccagacgcgc gatggcttca tcgacagccg cctttgcctc gtcaaccgat
     1921 ccgatacgat aataggcatc ggccagcaga cgcagagcga tgcccgcttc ataattggcc
     1981 ccgctggcga taggcagccc gtatacctcg acttgttctt ctccggcagc gaccggctta
     2041 taagcctcca gcgccaggcg tgcctcggca atggccgact cgacggcggc cacccggtcg
     2101 ccggtgaaat cctcggcctg cgaatcgtcc aacagagttt gcgcccgcag ataatggac
//
LOCUS       DAOMQG010000491         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_19985_length_2157_cov_9.429591, whole genome shotgun sequence.
ACCESSION   DAOMQG010000491 DAOMQG010000000
VERSION     DAOMQG010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19985_length_2157_cov_9.429591"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLC06_16395"
     CDS             complement(<1..319)
                     /locus_tag="PLC06_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRO92082.1"
                     /translation="MQTSHPEIDPQAARLFDDFRVIAHQIHQLSENSLDASGLSYAQY
                     RVLMILRFCEWDGKCDGLNPSEISATQGTSRNTISSLIRSLEEGGYIERQLDSDDRRR
                     FNIH"
     gene            604..1365
                     /locus_tag="PLC06_16400"
     CDS             604..1365
                     /locus_tag="PLC06_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRO92083.1"
                     /translation="MTSPVYYPLEPRFRAIVVGASSGLGAALVRQLASQRYHVAAVAR
                     REANLAALRDSVGKDLVLPYVHDVTEYEQVPMLFDQITRDLGGLDLIIYCAAVQPPVA
                     LHEYNFEKDEAMVTTNFLGAVAWLNQAALRFERARAGHIVGISSIAGDRGRVGSPAYN
                     ASKAGLDTYLEALRNRLSRYGIVVTTIKPGFVDTDLLKNAPKTFWVVSPEEAAARTLT
                     AIRRRRQVAYVPSRWQFVSLVVRSVPSFIFRRLSF"
     gene            1382..>2157
                     /locus_tag="PLC06_16405"
     CDS             1382..>2157
                     /locus_tag="PLC06_16405"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRO92084.1"
                     /translation="MTDIKSPSTRKLMPHSTQKYAALSDKHLEQVSSWGEATSGMSYV
                     YRPSTVEALRDILALAKQSGRTVGLRGGGNSYGDAAMNSENILIDMCRLRRILEWDPA
                     TGRIRVEPGVTLAELWQYIIEDGWWPPVATGTSATTMGGSAAMNVHGKNAFKVGPIGD
                     HIIEFDLMLTSGEVITCSREENSDVFHAAIGGFGMLGLFISLTLQMKRIYSGLLDVET
                     SSRRDLGEMFAYFREHAADSDYVVGWIDSLATGKSLGRGDV"
BASE COUNT          452 a          574 c          630 g          501 t
ORIGIN      
        1 ggtgaatgtt gaatcgacgg cgatcatcgc tgtccagttg tcgctcaatg taaccacctt
       61 cttccaaaga gcgaattagg gagctaatgg tgttgcggct ggtgccctgt gtggcgctga
      121 tctccgaagg attcaggccg tcgcatttgc cgtcccattc acagaaccgc aaaatcatga
      181 gtacgcgata ttgggcatag gatagccccg acgcatccag actgttttcg ctcaattggt
      241 gaatctgatg ggcgatcacg cgaaaatcat caaataaccg agccgcttgc gggtcgattt
      301 cgggatgaga tgtttgcatg aaagaaagcc atctctcgcg catttccttg gtgaacctgc
      361 tgtgagctga catagtaata cctgatagat aagtatataa cagaacgaat tatacgacga
      421 cgaactatat tgtcaatagc gttctcatag atatgtcgca ggtgaagcgg cgctttacag
      481 cgacgttgaa tctgatagaa tcggtttctg aggcgttcac tagtctcagg agcctcgggt
      541 tgccttgccc gctgcggcat caaattcggc cgcttatcct ccgaatcttg accaatcata
      601 attatgactt cacccgttta ttacccattg gaaccccggt ttcgtgcaat tgtcgttggc
      661 gcctcctcag ggctgggcgc ggcgttggtg cgtcagttag cgagccaacg ctatcacgtg
      721 gcggccgtcg cccggcgcga agccaatctg gctgctttgc gcgatagcgt cggcaaggat
      781 ctcgtcttgc cctatgtcca tgacgtcacg gagtacgagc aggtgcccat gctgtttgac
      841 cagatcactc gcgatctcgg tggcctggat ctgattatct attgcgcggc cgtgcagccc
      901 cccgtcgctc ttcatgagta taatttcgag aaagatgaag cgatggtgac caccaacttc
      961 ctgggagcgg tcgcctggct taatcaggcc gccttgcgct ttgagcgcgc gcgagcgggc
     1021 catattgtcg gcatcagctc gattgccggc gatcgcggac gtgtcggcag tccggcctat
     1081 aatgcctcga aagccgggtt ggacacgtat ctggaagcac ttcgcaatcg gttgtcgcgg
     1141 tatgggatcg tagtgacgac gatcaagccg ggcttcgtgg atacggattt actgaaaaat
     1201 gcccccaaga cgttttgggt ggtgtcgccg gaagaagcgg cggccaggac gctcactgcc
     1261 attcgtcgcc gtcgccaggt tgcctacgtg ccgtcgcggt ggcaatttgt gagcctggtc
     1321 gtgcgctcgg tcccttcttt tatcttccgg cgcttgagct tctagaaaac ggaaaagagc
     1381 catgaccgac ataaaatccc catcaacccg caagttgatg cctcattcaa cccaaaagta
     1441 tgccgcgctt tccgacaagc atctggagca ggtctcgtcc tggggcgagg cgaccagcgg
     1501 gatgagctat gtctatcgtc ccagcaccgt tgaagctctg cgcgacattt tggctctcgc
     1561 caaacagagc gggcgcacgg taggcttgcg gggtggcggc aatagctatg gcgatgcggc
     1621 gatgaacagc gaaaacatcc tcattgatat gtgccgcttg aggcgaatcc tggaatggga
     1681 tcccgcaaca ggacgtattc gagtcgagcc gggggtgact ttggctgagc tgtggcaata
     1741 catcattgag gacggctggt ggccgcccgt cgccacggga accagcgcga ccacgatggg
     1801 tggttcggcg gcgatgaatg tccacggcaa aaacgcgttc aaggttggac caatcggcga
     1861 tcatattatc gagttcgatc tgatgctgac gtcgggcgaa gtgatcacgt gcagccgcga
     1921 agaaaacagc gacgttttcc atgcggccat tggtggcttt ggcatgttgg gcctgttcat
     1981 tagcctgacc ctgcaaatga aacgtattta ctccgggctg ctcgatgtgg aaacctcgtc
     2041 ccgtcgcgac ctgggcgaga tgttcgccta cttccgcgaa cacgcggccg actccgatta
     2101 cgtcgtaggc tggatcgatt ccctggctac cggcaaatcg ctggggcgag gtgatgt
//
LOCUS       DAOMQG010000492         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20006_length_2155_cov_8.767619, whole genome shotgun sequence.
ACCESSION   DAOMQG010000492 DAOMQG010000000
VERSION     DAOMQG010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20006_length_2155_cov_8.767619"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /gene="topA"
                     /locus_tag="PLC06_16410"
     CDS             complement(<1..2045)
                     /gene="topA"
                     /locus_tag="PLC06_16410"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRO92085.1"
                     /translation="MSKPEPSARKAAPRKAAAKKSKATKGSTKSTGKGSIRHGEKLVV
                     VESPAKAKTIGRYLGRGYVVKSSLGHVRDLLKSRLSVDVDNNFEPEYRVPIDRRTVVK
                     ELKEAAAHSSEIFLATDPDREGEAIAWHVMEAAEMDPARTKRVVFQEITQPAIRAAFE
                     HPRDIDMDRVEAQQARRILDRLVGYQLSPLLWRKVQGRLSAGRVQSVAVRLVVERERE
                     IESFDSVEYWSINAELSQTKFRVDPRGNGNRRPYFIGRLHRINGEEPELGSNEVVDPH
                     IKALGEAHWEAGDVNIGTRTRRPAAPFTTSTLQQDSSRRLGFGTSRTMKIAQELYEGV
                     NVGEGDTGLITYMRTDSVNVAGIAQEQAREFIQGTFGSEYLPDKPPVYKTRSKTAQEA
                     HEAIRPTSVERTPAALKPHLSKDQFRLYKLIWERFVASQMAPALYDTVTAEIWAGPAA
                     VPAEKRPYLFRASGSTLTFRGFLAVYGTEDPDEENGDDPNEPQIPSDLVNGEPVDLLK
                     LLPEQHFTQPPPRFSEASLVKAMEEHDIGRPSTYASIISTIQNRGYVVQEQKRLQPTE
                     IGVIVNDMLVQYFPDVVSVDFTAKLEDELDEIAEGKPWVPVIGDFYRQFERDLATADA
                     ALPKLDLKKEPEFVGRDCPLSGHPLVYRQGRFGRFIGCSNFPHCHYTEQILVKTGAT"
BASE COUNT          456 a          627 c          566 g          506 t
ORIGIN      
        1 caggtagctc ccgtttttac cagtatttgt tcggtgtaat ggcaatgcgg gaagttgctg
       61 catccaatga aacggccgaa acgaccttgc cggtaaacca atgggtgtcc actcagaggg
      121 cagtcccgac cgacaaactc aggttctttc ttgaggtcca gtttgggcag cgcagcatcg
      181 gccgtcgcca gatctcgctc gaattgtcga taaaaatccc cgatgacggg aacccagggt
      241 ttgccctcgg caatctcatc caactcatct tccaacttcg cggtgaaatc gactgatacg
      301 acatcgggaa agtactgaac cagcatatcg ttcacgatca caccaatctc tgttggttgc
      361 aggcgctttt gctcctgcac cacataaccg cgattttgaa tggtggagat gatagaagca
      421 tatgtagagg gtcggccaat gtcatgctct tccatcgcct tgaccagtga ggcctcagaa
      481 aaccgtgggg gtggctgcgt gaaatgttgt tcaggcaaca gcttcaaaag gtcaactggc
      541 tcgccattca ccagatcgga tggaatctgt ggttcgtttg gatcatcacc attttcttca
      601 tcaggatcct ctgtcccata aacggcgagg aatccccgaa aggtcagcgt cgatccggaa
      661 gccctaaaca aataaggacg tttctcagcc ggcacggcgg caggacctgc ccatatctcg
      721 gccgtcaccg tatcatacaa ggctggcgcc atttggctgg ccacaaaccg ctcccaaatt
      781 agcttataga ggcgaaattg atccttggac aggtgaggtt taagcgctgc cggagttcgt
      841 tcgacagatg tcgggcgaat tgcctcatgc gcttcctggg ctgtcttgga gcgcgtttta
      901 tacacgggtg gtttatcggg caggtattca gagccgaatg tcccctggat gaactcgcgc
      961 gcctgttcct gggcaatgcc ggccacgttg acgctatctg tacgcatata ggtgatgagg
     1021 ccggtatcgc cctcgccaac attgaccccc tcatacagct cctgggctat tttcatcgtg
     1081 cgcgacgtgc cgaagccgag acggcgcgat gaatcctgct gaagcgtgct tgtggtaaaa
     1141 ggggcggccg gccgccgagt acgggtaccg atattaacgt cgccggcttc ccaatgtgcc
     1201 tcaccaagag ctttaatatg aggatcaacg acttcattgc tccccaactc aggctcttca
     1261 ccattaattc gatgtaaccg gccaatgaag tatggacggc gattgccgtt gccacgagga
     1321 tcaacccgga acttcgtctg gctcaactcg gcattgatag accaatattc aactgaatcg
     1381 aatgactcga tctccctctc gcgctcaact accaggcgca cagccaccga ttgaacacgc
     1441 cctgccgaga gtcgcccttg caccttccgc cacaggagag ggcttagctg gtagccaacc
     1501 agccgatcca ggatacggcg cgcttgttgc gcttccactc gatccatatc gatatcgcgc
     1561 ggatgctcaa atgctgcgcg tatggccggt tgggtgatct cctggaagac gactcgtttg
     1621 gtccgggccg gatccatttc ggcagcttcc atgacgtgcc aggcaatggc ttcgccttct
     1681 cgatcaggat cggtggccag gaagatttcg ctcgaatggg cggccgcttc cttaagctcc
     1741 ttgacaaccg tccgtctgtc aattggcaca cgatattccg gctcaaaatt attgtcgaca
     1801 tcgacactga gtctcgattt cagcaggtcg cgcacatgtc ccaggctcga cttaaccaca
     1861 taaccgcggc cgaggtatcg cccgatcgtc ttggccttgg ccggagactc aactactacc
     1921 agcttctcgc catggcgaat cgaacctttg ccggtggatt ttgtgctccc ctttgtggcc
     1981 ttactcttct tggcagcagc cttgcgcgga gccgcctttc gtgccgacgg ctcaggcttg
     2041 gacaatgtct cgtgagcctc tgtgcgccct tgcttataaa tagttccgcc gcatacagga
     2101 cattttccgc gcgtccccgg cgatccattc gccgcccact cggggatcgg atcga
//
LOCUS       DAOMQG010000493         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20035_length_2152_cov_9.616118, whole genome shotgun sequence.
ACCESSION   DAOMQG010000493 DAOMQG010000000
VERSION     DAOMQG010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20035_length_2152_cov_9.616118"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLC06_16415"
     CDS             complement(<1..218)
                     /locus_tag="PLC06_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRO92086.1"
                     /translation="MIAPSQIDMRSRILGLDHEVPLLDGRRVPYVNLDNAASTPPFID
                     VVRAIEAFMPYYSSVHRGTGFKSRLSTVA"
     gene            complement(244..849)
                     /gene="recR"
                     /locus_tag="PLC06_16420"
     CDS             complement(244..849)
                     /gene="recR"
                     /locus_tag="PLC06_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRO92087.1"
                     /translation="MAQSVLPRTVQRLIDEFARLPGIGPKSASRLTFYLLRAAGNQAI
                     DLATALQDLKEHTRLCSICFNITEDDPCAICDDEARDARLLCVVEEPLDVLAIERARA
                     YGGRYHVLHGAISPVEGIGPDDLRIDELVARVDKGDFQEIILATNPTLEGESTALYLQ
                     RRLSDRNVKLTRLARGLPVGGDLEYTDEITLGRAFEGRQNI"
     gene            complement(853..1224)
                     /locus_tag="PLC06_16425"
     CDS             complement(853..1224)
                     /locus_tag="PLC06_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRO92088.1"
                     /translation="MTKRSFHNKTKKKSASPNDMLGQMQKMQQDMAAAQSALENETVT
                     VTAGGGAISVTITGHQRLRALTINPELLSPDEAEFLQDMLIAGINAAIEQSQALAAQR
                     MEGITGGVGGMDGLLGGLGLG"
     gene            complement(1308..>2152)
                     /locus_tag="PLC06_16430"
     CDS             complement(1308..>2152)
                     /locus_tag="PLC06_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92089.1"
                     /translation="QFCRQMVAHLRQLLMLQASGNIEVDGPVERRAEMLAQARRAPRK
                     ALIDAVRRFHEASMQPAGSWQPQLPLELAFLELVPEVITNYELRVTNEETADRRPATA
                     GHGQQTTDDRLLTTGHRPQQEHPVAVGSRPSAADDRPQTTAEETSSVDDRSPQEPPTA
                     VVGRPSAVGDSALTVAGIAAMWPEMTKRVGRRNKNLPALLAMCKPLALEGNTIILGFD
                     YPLLKDKFDKTAGALDLVVDTFRALGAADCTARTVTTSEYPMPIAREEFQALASELGG
                     VVKN"
BASE COUNT          360 a          686 c          678 g          428 t
ORIGIN      
        1 gcgaccgtgc tgaggcgtga cttgaacccc gtgccgcgat ggacgctgga gtagtagggc
       61 atgaacgcct cgatggcgcg cacgacgtcg atgaacggcg gcgtgctggc ggcgttgtct
      121 agattgacat aaggcacgcg acggccgtcg agcaggggca cctcgtgatc cagtcccaga
      181 atccgcgagc gcatatcgat ctgtgatgga gctatcatcg ttattcctcc gggaatagat
      241 atttcagatg ttttgccggc cttcgaaggc gcggccgagg gtgatctcgt cggtgtactc
      301 caggtcgccg ccgacgggca acccccgcgc gaggcgggtc agcttgacgt tgcggtcgct
      361 caggcggcgc tgcagataga gggccgtcga ttcgccctcc agcgtcggat tcgtggccag
      421 gatgatctcc tgaaaatcgc ctttgtccac gcgagcgacc agctcgtcga tgcgcaaatc
      481 atccggcccg atgccctcca ccggcgaaat agccccgtgg agcacgtgat agcggccgcc
      541 ataggctcgc gcgcgctcga tggccagcac gtccagcggc tcctcgacga cgcacagcag
      601 gcgggcgtcg cgcgcctcgt cgtcacagat ggcgcaggga tcatcctcag tgatattgaa
      661 acagatcgaa cagagccggg tgtgttcctt caagtcttgc agcgccgtcg ccagatcgat
      721 ggcctgattg ccggccgcgc gcagcaaata gaacgtcagc cgcgaggccg acttggggcc
      781 gatgcccggc aggcgggcga actcgtcgat gagccgctgc acggtgcgcg gcagcacgga
      841 ttgggccacg gcctagccta accccagacc gccgagcaga ccatccatcc cgcccacgcc
      901 gccggtgata ccttccattc gctgcgcggc cagcgcctgc gattgttcga tggcggcgtt
      961 gatcccggcg atgagcatat cctgcaggaa ttcggcttcg tccgggctga gcagctccgg
     1021 gttgatagtc agggcgcgca gccgctggtg gccggtgatg gtgacggaga tcgccccacc
     1081 gccggcggtg accgtcaccg tctcgttctc cagcgccgac tgcgccgcgg ccatatcctg
     1141 ctgcatcttc tgcatctgtc ccagcatatc gttgggcgac gcggatttct tcttggtttt
     1201 gttatggaag gatcgcttgg tcatctattc ccctttttta agtacgaggt acgaaggacg
     1261 aagtacgaac gacgcgcact tcattcctga ttcctgattc gtaattccta attcttcacc
     1321 actcccccca actccgaggc cagcgcctgg aactcttcgc gggcgatggg catggggtat
     1381 tcggaggttg tcacagtgcg ggcggtgcaa tcggccgcac ccagcgcgcg gaacgtatcg
     1441 accacaaggt cgagcgcccc ggccgtcttg tcgaatttgt ctttcagcaa cggataatcg
     1501 aagccgagaa tgatggtgtt gccctcaagc gccagcggct tgcacatcgc cagcagcgcg
     1561 ggcaggttct tgttgcggcg gccgacccgt ttggtcatct ccggccacat tgcagctatg
     1621 ccggctacgg tcagagcact gtcgccgacc gccgacggcc gaccgacgac cgctgtcggc
     1681 ggctcttgcg gcgatctgtc atcgacggac gatgtttctt ccgccgtggt ttgtggtctg
     1741 tcatcggccg ccgacggtcg actgccgacc gccaccggat gttcttgctg tggtctgtgg
     1801 ccagtggtca gtagtctgtc gtcagtggtc tgctgtccgt ggccggcggt cgccggtctg
     1861 cggtcggccg tctcttcatt cgtaactcgt aattcgtaat tcgtaattac ttccggcacc
     1921 agttccaaaa acgccagctc cagcggcaac tgcggctgcc aactccccgc cggctgcatc
     1981 gacgcttcat ggaagcggcg cacggcatcg atgagcgcct tgcgcggggc gcggcgggct
     2041 tgggccagca tctccgcccg acgctcgacc gggccgtcaa cctcaatgtt gccgctggcc
     2101 tggagcatca gcaactgccg caggtgggcc accatctggc ggcagaactg gc
//
LOCUS       DAOMQG010000494         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20152_length_2142_cov_10.526114, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000494 DAOMQG010000000
VERSION     DAOMQG010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20152_length_2142_cov_10.526114"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLC06_16435"
     CDS             <1..1326
                     /locus_tag="PLC06_16435"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRO92090.1"
                     /translation="GDALPGAPVYHMAITIDPSMTLITGRQTVTYTNNESVDLNEAYF
                     HLHAKTLDGDITVSAVTVDGAPAAAEVVGDMLRVPLAAPLAPGDVTTIDMAFETTVPT
                     DIGRNYGVLAYYDDILALAHFYPMLAVYDEEGWNTTAPDVQGDLTYSDSAFYLVRVTA
                     PDGLALVATGSIVDETVTDGTQTLTFAAGPARDFYLVAGDFAVVSETVGETTINSYAP
                     PDMADGAAMALDVAAAALESATERLSAYPYRELDIVTTPTSALGIEYPGLIVGTVNMY
                     DISATTSAGVPYEAILESTIAHEVMHQWFYNLVGNDQLDEPWLDESVVGYSTYRYFVD
                     RYGQDVGDNYFQNFLGRWDRVERAEIPIGRPVAEYEGAEYSAIVYGRGAVFVRELEEA
                     MGREAFDAFLRAYVTRFRWDVATTADFRAMAEEQCGCDLGELFAKFINE"
     gene            complement(1630..2064)
                     /gene="aroQ"
                     /locus_tag="PLC06_16440"
     CDS             complement(1630..2064)
                     /gene="aroQ"
                     /locus_tag="PLC06_16440"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRO92091.1"
                     /translation="MRILVLHGPNLNLLGQREPEVYGRWTLAEIDEMLIEEAHKSNTE
                     LRIMQSNHEGALIDAIHDARLWGQGILINPGALTHYSYALRDAIAAVGLPTVEVHLSN
                     IHARERFRRQSVIAPVCVGQICGFGRYSYRLGLAALLARLPA"
BASE COUNT          408 a          717 c          624 g          393 t
ORIGIN      
        1 ggcgacgccc tgcccggcgc gccggtctat cacatggcca tcaccatcga cccgtcgatg
       61 accctcatca ccggccgcca gaccgtcacc tataccaaca acgaaagcgt tgacctgaac
      121 gaggcctact tccacctgca cgcgaaaacg ctcgacggcg acatcaccgt gtcggccgtc
      181 accgtcgacg gcgcgcccgc cgcggccgag gtcgtgggtg acatgctgcg cgtgccgctg
      241 gccgccccgc tggcccccgg cgacgtgacg accatcgaca tggccttcga gacgaccgtg
      301 ccgaccgaca tcggccgcaa ctatggcgtg ttggcctact acgacgacat cctggccctg
      361 gcccacttct acccgatgct ggccgtctat gatgaagaag gctggaacac gaccgcgccc
      421 gacgtccagg gcgatctgac ctacagcgat tcggccttct acctggtgcg cgtcaccgcc
      481 cccgacggcc tggccctcgt cgccacgggg tcgattgtgg atgagaccgt caccgacggc
      541 acgcagacgc tcaccttcgc cgccggcccg gcccgcgatt tctacctggt cgccggcgat
      601 ttcgccgttg tcagcgagac cgtgggcgag acgaccatca acagctacgc cccgcccgac
      661 atggccgacg gcgcggcgat ggccctcgat gtggccgcgg ccgccctgga gagcgccaca
      721 gagcgcctgt ccgcctatcc ctatcgtgaa ctcgacatcg tcaccacgcc gacgtcggcc
      781 ctgggcatcg aataccccgg cctcatcgtc ggcacggtca acatgtacga catcagcgcg
      841 acgactagtg cgggcgtccc ttatgaggcg atcctcgaat cgaccatcgc ccacgaggtg
      901 atgcaccagt ggttctataa cctcgtcggc aacgaccagc tggacgaacc gtggctcgat
      961 gagtccgtcg tcggctactc cacctatcgc tatttcgtcg accgctacgg ccaggacgtg
     1021 ggcgacaact acttccagaa cttcctcggc cgctgggatc gggtggagcg agccgagatc
     1081 cccatcggcc gtcccgtggc ggagtatgag ggcgcggaat acagcgccat cgtatacggc
     1141 cgcggggcgg tgtttgtgcg cgaactggaa gaggcgatgg ggcgcgaagc attcgacgcc
     1201 tttctgcgtg cctacgtgac ccgcttccgg tgggatgtgg ccacgacagc cgatttccgg
     1261 gccatggccg aggagcaatg cggctgcgat ctgggcgagc tgttcgcaaa attcataaat
     1321 gagtaataat acctgcgaca aatgatggat tgtgcggatt tacaagccga cattttaatt
     1381 atataaatag ggtatatctt ggcgaggacg tggaatagcg gcagcttcca gatccaatat
     1441 tgccaacatc gcaacaatca acgtagtctg catcgcagcc aacaccgccg caacaccgcg
     1501 attcgcatca cgttcagtat tgccccaagc attgcttctt gatgcgccca cggccaggac
     1561 gagatatgaa aaaaagggcc ggacatcccc ccatgtccgg cctttttttg ttgttccgac
     1621 ggcaatcccc tatgccggca atcgagccag cagtgccgcc agccccaacc ggtagctata
     1681 tcgcccgaag ccgcaaatct gcccaacgca taccggcgca atgaccgact gccgccggaa
     1741 ccgctcacgg gcgtggatat tggacagatg cacctcgacc gtgggcaatc ccaccgcggc
     1801 gatggcgtcg cgcagggcgt agctgtagtg ggtcagcgcg ccggggttga tcaggatgcc
     1861 ctgcccccac aggcgcgcgt cgtggatagc gtcgatgagc gcgccctcgt ggttcgactg
     1921 catgatccgc agctcggtgt tgctcttgtg ggcttcttcg atgagcatct cgtcaatctc
     1981 ggccagcgtc cagcggccgt acacttccgg ctcgcgctgc cccaacagat tcagattagg
     2041 gccatggaga acgaggattc gcatgggaaa ctccttttta gtggcgagtt actcttctgt
     2101 cgtcattctt gccgcttcca gcgccgccag cacggccgct tc
//
LOCUS       DAOMQG010000495         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20281_length_2130_cov_6.405301, whole genome shotgun sequence.
ACCESSION   DAOMQG010000495 DAOMQG010000000
VERSION     DAOMQG010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20281_length_2130_cov_6.405301"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="PLC06_16445"
     CDS             complement(<1..1659)
                     /locus_tag="PLC06_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRO92092.1"
                     /translation="MRRRNIFQLYQDEFSGYNLSIFRQDLLAGLTVAAVALPLALAFG
                     VASGATAAAGLVTAIISGLLIGFLSGAPYQISGPTGAMSAVLILLVARYGLPGMWIAG
                     LMAGVLLLIIGLLRLGRFIAYIPSPVITGFTSGIALIIFIGQIDHFLGITTTGGEEVS
                     TLLKLLSYFRGGFVVDWRAVALGMLVILTMVFWPARWNARFPSSLLGLIVATAAGVVL
                     GWPVPEIGEIPQTLLLADRLGFDQIPWGELTNFIGPALTITALGAIESLLCGAVAANM
                     TGIRLQANQELIAQGVGNIVIPFFGGVPATAAIARTSVGIRAGGHTRMVSIIHALGLL
                     LSMFLLAPIMGRIPLAALAGVLMVTAVRMNEWEAIRFIFGRRFKTGMITFLITLFATI
                     SLDLTQAILIGVFISGAIFLSQIAVLDVEVQEVDPEKLRRRGIETAGKCNHVRVGYLT
                     GPLFFAATGSFNEAFARLGDTHALILSMRGVPLIDTSGLEAIRRLDEQLRERGGVLMF
                     AGVHKNALRMMERAGLVEEIGPENFFWSSDQAIVAAEARGCRFCE"
     gene            1882..2097
                     /locus_tag="PLC06_16450"
     CDS             1882..2097
                     /locus_tag="PLC06_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92093.1"
                     /translation="MARRGDTERLDKIRDAIINNPGQRPGWVARQLGFDNKTIMRALT
                     QLEDRGEMLIEDERGRLSWFGRRHGEG"
BASE COUNT          417 a          690 c          703 g          320 t
ORIGIN      
        1 ctcacaaaac cggcatcctc tcgcctcggc ggccacaatc gcctgatcgc tgctccagaa
       61 gaagttctcc gggccgatct cctccaccag cccggcccgc tccatcatcc gcagggcgtt
      121 cttgtgcacc ccggcgaaca tcagcacgcc gccgcgctcg cgcaattgct cgtcgagccg
      181 ccggatcgcc tccagcccgg acgtgtcgat cagcggcacg ccgcgcatcg acaggatcag
      241 cgcgtgggtg tcgcccagcc tggcgaatgc ctcgttgaag ctgcccgtgg cggcgaagaa
      301 cagcggcccg gtgagatagc cgacgcgcac gtgattgcat ttgcccgccg tctcgatccc
      361 ccgccggcgc agcttttcgg ggtcgacctc ctgcacctcg acatcgagga cggcgatctg
      421 gctcaggaag atcgcgccgg agatgaacac accgatcagg atggcctggg tcaggtcgag
      481 cgagatggtg gcgaacaggg tgatgaggaa ggtgatcatg cctgtcttga agcggcggcc
      541 gaagatgaag cgaatcgcct cccactcgtt catgcgcacg gcggtgacca tcagcacccc
      601 ggccagcgcc gccaacggga tacgccccat gatcggggcc agcagaaaca tcgacagcag
      661 cagccccagc gcgtggatga tgctgaccat gcgcgtgtgg ccgccggcgc ggatgccgac
      721 gctggtgcgg gcgatggccg ccgtggccgg cacgccgccg aagaacggga tgacgatgtt
      781 gcccaccccc tgggcgatga gttcctgatt ggcctgcagg cgaatcccgg tcatgttggc
      841 ggccaccgcg ccgcacagca ggctctcgat tgcccccaac gcggtgatgg tcagcgccgg
      901 gccgatgaaa ttcgttagct cgccccaggg aatctggtcg aaccccaggc ggtcggccag
      961 cagcaacgtc tggggaatct cgccgatttc gggcaccggc cagcccagca cgacgcccgc
     1021 ggccgtggcc acgatgaggc ccagcagcga cgacgggaag cgcgcgttcc agcgggcggg
     1081 ccagaacacc atcgtcagga tgacgagcat ccccagcgcc accgcccgcc aatcgacgac
     1141 gaagccgccc cggaaatagc tcagcagctt gagcagggtg gagacttcct caccgcccgt
     1201 ggtggtgatg cccagaaagt ggtcgatctg gccgatgaag atgatgaggg cgatgccgga
     1261 cgtgaaaccg gtgatgaccg gcgacgggat gtaggcaatg aagcggccga gtcgcagcag
     1321 gccgatgatg agcagcagta cgcccgccat gaggccggcg atccacatcc ccggcaggcc
     1381 gtagcgggct acgagcagga tgagcacggc gctcatggcc cccgtcggcc cggaaatctg
     1441 atagggcgcg ccggacagga agccgatgag caggccggag atgatggccg tcaccagtcc
     1501 ggcagcggcc gttgcgcccg aagccacgcc gaaggccagc gccagtggca aggccacggc
     1561 ggccacggtg aggccggcca gcaaatcctg ccggaagatc gacaggttat agcccgagaa
     1621 ttcatcctga tagagctgaa atatgtttcg tcgtcgcatc gcccacccat ttctcgtcgc
     1681 gggcaatccc gtttcgctcg tcgcccggcg taattggctc gcattatcga tgaaatgacc
     1741 ccaatttgcc aaattgcaac ggatttcgct gcgctgttgc gccccttgca acagggcgat
     1801 cctaccctca gggcataacc ttccagcgtg gcccggcgga cgaccggccg ggtacaaaat
     1861 atgaccggat aggggtggac catggctcgt agaggcgata cggaacggct ggacaagatt
     1921 cgtgatgcga tcatcaacaa cccggggcag cggccggggt gggtggcccg gcagttgggc
     1981 ttcgacaaca agacgatcat gcgcgcgctc acccaattgg aagatcgcgg ggagatgctg
     2041 atcgaggacg agcgcggccg cctgtcctgg ttcggccgcc gccatggcga agggtagcgc
     2101 agacaggaat gtctgcgcta cagttgtggc
//
LOCUS       DAOMQG010000496         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20355_length_2123_cov_7.373791, whole genome shotgun sequence.
ACCESSION   DAOMQG010000496 DAOMQG010000000
VERSION     DAOMQG010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20355_length_2123_cov_7.373791"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PLC06_16455"
     CDS             complement(<1..1650)
                     /locus_tag="PLC06_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92094.1"
                     /translation="MNHHFIGTRRAIVALCLGLLVVALGTLTASAAAITVTPGSPQGW
                     ASTGNSGGGSSAITTTAPRSGLGSTELTGDRTRWIKAVDPLAPAKLGDLYAVTDFAFD
                     WMVATGSTSSLPQPPDQTPGLRLHVYDPNATNKYSELIWEGAYNGPVTVTQGSWVSTD
                     MIGGGQLLWRWVTGPGITLDGGGSQVNQTLSAWQNSAWYSSDAVVFGVSVGVGSSIGA
                     GYHAFTDNVVLGFSNAPDTYNFEPNPVCTTVCYADAVNGNDANGGTSVADAKKTIQAA
                     IDAVSAGGQVRVLPGSYSETATNRWVLGINGPHQFGLFVEDNTKDGISILGVTAADVE
                     ITDPSMTLADITTNATNNFGYSGIFVEADNVTISGLEIGANTPWDNKTIEVIGDAFTF
                     KNSFMNVAAGGSLYFGDWRFDTGTNTSYMLSYEIDNNIFSDGTSVDITSGAGYSGATA
                     GKKITNNEFEFSLAQYTASGNYSWPYVSFTGDSTTVPWFVYPMTGAVITGNTFNNTAP
                     NGIHIRARGIADDSTFDWESYWNDNTFNKAVIVGPTLFSDVR"
BASE COUNT          392 a          624 c          690 g          417 t
ORIGIN      
        1 gcgcacgtcg ctgaacaggg tcggcccgac gataaccgcc ttgttgaagg tgttgtcgtt
       61 ccagtacgat tcccaatcaa acgtcgagtc gtcggcaatg ccgcgggcgc ggatatggat
      121 gccgttgggg gccgtgttgt tgaaggtgtt gccggtgatg accgccccgg tcatcggata
      181 aacgaaccac ggcacggtgg tcgagtcgcc ggtgaagctg acatacggcc acgagtagtt
      241 gccgctggcc gtatattgcg ccagggagaa ctcgaactcg ttgttcgtga tcttcttgcc
      301 ggccgtcgcg ccgctataac ccgccccgct ggtgatgtcc acgctcgtgc cgtcgctgaa
      361 gatgttgttg tcgatctcat agctcagcat gtaggacgtg ttcgtgccgg tgtcgaagcg
      421 ccagtcgccg aaatagagcg acccgccggc ggcgacgttc atgaacgagt tcttgaacgt
      481 gaaggcgtcg ccaatcacct cgatggtctt gttgtcccag ggggtgttcg cgccgatctc
      541 caggccgctg atggtcacat tgtcggcctc gacgaagatg ccactgtagc cgaagttgtt
      601 ggtggcgttg gtggtgatgt cggccagggt cattgacggg tcggtgatct caacgtcggc
      661 cgccgtcacg ccgaggatgc tgatgccgtc cttcgtattg tcctcgacga acagaccgaa
      721 ctggtgcggg ccgttgatcc ccagcaccca gcggttggta gccgtctcgc tgtagctgcc
      781 gggcagcacg cgaacctggc cgccggcgct gacggcgtcg atagccgcct gaatcgtctt
      841 cttggcgtcg gccacgctgg tgccgccgtt ggcgtcgttg ccgttcacgg cgtcggcata
      901 gcagacggtg gtgcaaaccg ggttcggctc gaaattgtag gtgtcggggg cgttgctgaa
      961 tcccagcacc acgttgtcgg tgaaggcatg gtagcccgcg ccgatcgacg acccaacgcc
     1021 gacgctgacg ccgaacacga cggcgtcgct gctgtaccac gctgaattct gccacgcgct
     1081 tagcgtctgg ttcacctgcg agccgccgcc gtccagcgtt atgcccgggc cggtcaccca
     1141 gcgccacagc agctggccgc cgccgatcat gtcggtgctg acccagctgc cctgcgtcac
     1201 ggttacaggg ccgttgtagg ccccttccca aatcagttca ctatatttgt tggtcgcgtt
     1261 cgggtcatag acatggagac gcaacccggg ggtctgatcc ggtggctgcg ggaggctcga
     1321 cgtgctgccc gtggccacca tccagtcgaa cgcaaaatcc gtcaccgcgt acagatcgcc
     1381 cagcttggcc ggcgcgagcg gatccacggc cttaatccac cgggtgcgat cgccggtcaa
     1441 ctccgtcgag ccgaggccgg agcgcggcgc ggtggtggtg atggcgctgg agccgccgcc
     1501 ggagttgccg gtggaagccc atccctgcgg actgcccggg gtgacggtga tagcggccgc
     1561 cgacgccgtc agcgtcccca gcgccacgac cagcaggccg agacacaagg ccacgatggc
     1621 gcggcgcgtt ccgataaagt gatggttcat tctatttcct ccaaactgaa tgttaacaac
     1681 gataaatctg acacaagcgg gcgaccggca tgcgccgaac gcccacagtt caacccgacg
     1741 cgccgaggcg tgccgaagcg accttttcga ttttcacgga ttgtggcaac tatcgactgc
     1801 gcggccggct aaggtgagag aatacgattc cgggcagcca atggataact gggtcgactg
     1861 gttctaatga taactagctg atccgatttt tatcctcccc cgctcggctt gtgcgagcgg
     1921 acgatatgct tcctgctgcg gccgattgcc cgcccgtcgc gactggattg cgcgagggac
     1981 aaggcacgac cctcatatta tactaatata actttcttac attttacctt acagatgggg
     2041 agaataacgc cacgggggaa ttgggaaggg gagaaagggg gaaggggaga aggggagaag
     2101 gggagaaagg gagaaagggg gaa
//
LOCUS       DAOMQG010000497         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20517_length_2111_cov_8.214494, whole genome shotgun sequence.
ACCESSION   DAOMQG010000497 DAOMQG010000000
VERSION     DAOMQG010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20517_length_2111_cov_8.214494"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2111
                     /locus_tag="PLC06_16460"
     CDS             <1..>2111
                     /locus_tag="PLC06_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92095.1"
                     /translation="ISTFIFAPTLAVYLGALAFGRRSAAAGRLFRAFLLIGLGLGLAF
                     FYTGGAVLEMDQVTLEMSTTTRNNDWRYNFASLGEILSPVVAEDPTLVNPPLLFRLGW
                     MPLLLAAIGATGLAWIKGRDGHAREQRLHVGLMVAGVAVYLFMALPVSRWLWEIMPLI
                     DFVQFPWRFVGRAALPVAFLAGVPFYYFDRRHLSAVGGRPSAVIPAVGGRWSTVILIL
                     TLALLIIETIPNLYPRMCYEEPFPTILTVHNYEHITGLVGVDPEGSYFPRTVEQRPTG
                     SPLEADFIAGRTPQRFDATALPDGAQAQNIVYERFGVTLTVDSPTPFTARYLSFAFPG
                     WSARVDGERVEILPESPSGLITFDVPAGTHTIAVDWGATPLRLALVGLSILSAVGVLG
                     VIVWSRRRPSAAATGQPQGTAFPLKRSELALLVLLGLGLLAFKTYVDRNEMALRRTAA
                     PPVSHAVTLQGGEMALNGFNLSRDRVPAGETFDIDMAWTAVAAPQVDYQSEISLVGSD
                     GLAWSQKGTERTRVFEDAAPTRLWSAGEWGWDSREVWTLTGAPPGIYDIVVTLFDKAT
                     LAPVTLVDTSIGQAVGPTAVIGQIEIVNPTDVPAFDPQYPADHALPDSALRLLGYNQD
                     RAEAAPGESVLLTLFWECAAPANCADFSLSLRDEAGVAAQIWALPAVREGLSAEAWPS
                     HGHLRGQHIVPLPASLTSGRY"
BASE COUNT          307 a          713 c          682 g          409 t
ORIGIN      
        1 atatatcaac cttcatcttc gcgccgacgc tggcggtgta tttgggggcg ctggctttcg
       61 gccgacgatc ggccgccgcc ggccgtctgt tccgcgcctt tctgcttatc ggcctcggcc
      121 tcggcctggc cttcttctac accggcggcg cggtgctgga gatggatcag gtgacgctgg
      181 agatgtcgac gaccacgcgc aacaacgact ggcgctataa cttcgcttcg ctgggtgaaa
      241 tcctgtcgcc ggtcgtagcc gaagacccga cgctggtcaa cccaccgctc ctgttccgtc
      301 tgggctggat gccgcttctg ttggcggcca ttggcgcgac cggtctggca tggatcaaag
      361 gtcgcgacgg ccatgcccgc gagcaacgcc tgcatgtcgg gctgatggtc gccggcgtgg
      421 ctgtctacct cttcatggcc ctgcccgtct cccgctggtt gtgggagatc atgccgctga
      481 tcgatttcgt gcagttcccg tggcgcttcg tcggccgcgc ggcgctgccg gtggcgttcc
      541 tggcgggggt gccgttttat tattttgacc gccgacatct ttcggcggtc ggtggtcggc
      601 cgtcggcggt catccccgcg gtcggcggtc ggtggtcgac cgtcattctt attctcaccc
      661 tcgccctgct catcatcgaa accatcccca acctctaccc gcgcatgtgc tatgaagagc
      721 cgtttccaac gatcctgacc gtccacaact atgaacacat caccgggctg gtcggcgttg
      781 acccggaggg cagctacttc ccgcgcaccg tggagcagcg gccgaccggc tcgccactgg
      841 aggccgactt tattgccgga cgaacgccgc agcgtttcga cgcaacggct ttgcccgacg
      901 gcgcacaggc gcaaaatatc gtctacgagc gcttcggcgt cacactgacg gtcgattcac
      961 ccacaccctt caccgcccgc tacctcagct tcgccttccc cggctggtcg gcccgtgtcg
     1021 acggcgagcg ggtggaaatt ctccccgaat cacccagcgg cctcatcacc ttcgacgttc
     1081 ccgccgggac gcacacaatc gcggtcgatt ggggggccac ccccttgcgc ctggctctcg
     1141 tcggcctgag catcctctcg gccgtgggtg tgctgggcgt catcgtttgg tcaagacgac
     1201 gcccatcggc cgcggcaacc ggtcaacccc aggggacggc tttcccgttg aaacgtagcg
     1261 agttggcctt gctcgtcctg ctcggcctcg gcttgctggc cttcaagacc tacgttgacc
     1321 ggaacgagat ggcgctgcgt cgaacggccg cgccgcccgt gagccacgct gtgacgcttc
     1381 agggcggcga gatggcgctc aacggcttca atctcagccg cgaccgggtt cccgcgggcg
     1441 agacgttcga catcgatatg gcctggacgg ccgtcgccgc gccgcaggtc gattatcaat
     1501 cggaaatctc gctggtcggc tccgacgggc tggcctggag ccaaaagggg acggagcgca
     1561 cgcgcgtttt cgaagatgcc gcgccgacgc gcctctggtc ggcgggcgag tgggggtggg
     1621 acagccgcga agtgtggacg ctgaccggcg cgccgccggg catctacgac atcgtcgtga
     1681 cgcttttcga caaggccacg ctggccccgg tcacgctggt cgatacgtcg attgggcagg
     1741 cggtcggccc gacggccgtc atcgggcaga tcgagatcgt gaatcccacg gatgtgccgg
     1801 cgtttgaccc gcaatatccg gccgatcacg cgctacctga ttcagcgctg cgcctgctgg
     1861 gctacaatca ggatcgagcc gaagccgcgc cgggcgagtc cgtcctgctg accctgttct
     1921 gggagtgtgc tgcgcccgcg aattgcgcgg acttctcgct gtcgctgcgg gatgaggccg
     1981 gcgtcgcggc gcaaatatgg gcgctgccgg ctgtgcgcga agggttatcg gccgaggcgt
     2041 ggcccagcca cggccacctg cgcgggcaac acatcgtgcc cctgcccgca agcctgacga
     2101 gtggtcgata c
//
LOCUS       DAOMQG010000498         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20530_length_2110_cov_9.289538, whole genome shotgun sequence.
ACCESSION   DAOMQG010000498 DAOMQG010000000
VERSION     DAOMQG010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20530_length_2110_cov_9.289538"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..754
                     /locus_tag="PLC06_16465"
     CDS             155..754
                     /locus_tag="PLC06_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRO92096.1"
                     /translation="MNNLLELIHTRRSIRRYTHDVIPADVVDQLLNAAIRAPSAHNRQ
                     PWRFVVIANEQTRYRLATAMGDQLRRDLAADGRSPEFIERDAGRSYMRLTSAPLLILL
                     SLTMADMDVYPDERRARNEAIMAAQSTAMAGQNIMLAAHALGLGACWVCAPLFCPDVV
                     RETLALPADWQPQGLITLGYPAEEKQKTRHPLESRVLYR"
     gene            complement(777..>2110)
                     /locus_tag="PLC06_16470"
     CDS             complement(777..>2110)
                     /locus_tag="PLC06_16470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRO92097.1"
                     /translation="LREAKNLHEVELISFRAGEVLEIGPFKVEPFHVAHSIPDCVGFG
                     ITTPVGLIVHTGDYKFDHTPIDSWPPDFAKLAEFSQRGVLALMADSTNADRPGWTESE
                     MDLLEGFEDVFRKAEGRIIVATFASLISRVTIAAAAAIKFNRKMAIAGRSMRDAVKIA
                     RKLGYLELPDDLIVDLGVAVKMPPSEVVIMVTGSQGEPSAVLGRLARGTHNQLSIQEG
                     DTVIFSAHPIPGNEELIYRTINQLYRRGATVLYDRIANVHVSGHASREEMKLLINLIR
                     PLFLIPVHGELRHLKLHAEMGKALGIPEERIAVVENGTPIELTPYSMTVHERLPGGYV
                     FVDGSGVGDIGWAEVRDRDRLAQAGYFFAVVRTNGHGELAGSPKLTTRGFVNEKEADD
                     LLKGAGDTIARVVREAHTAGRELTDGQVESALSRYLYDETGKRPVIEAVIV"
BASE COUNT          408 a          698 c          616 g          388 t
ORIGIN      
        1 cgcagattac aagcgctatc ataacgtcat cattcagcaa cacaaatagt cctccttttt
       61 ttctcctctt cccgcaaaaa agccctgcat cccccgcagg gcttttttgc gcccgaccaa
      121 ggttaatcct gacggatggg atagaatctc tcgcatgaac aatctgctgg aactgatcca
      181 cactcgccga tccattcgcc gctataccca cgacgtaatc ccggccgatg tggtcgatca
      241 gctattaaac gcggccatcc gggcgccgtc ggcccataac cggcagccgt ggcgcttcgt
      301 cgtgattgcc aatgagcaga cccggtacag gctggccaca gccatgggcg accaactccg
      361 gcgcgatctg gcggccgacg gccgatcgcc ggagttcatc gagcgagacg ccgggcgctc
      421 ctatatgcgc ctgaccagcg cgccgctgct gattctgctc agcctgacga tggccgacat
      481 ggacgtctac cccgacgagc gccgcgctcg caatgaagcg atcatggcag cccaaagtac
      541 ggccatggcc ggacaaaaca tcatgctggc cgctcatgcc ctgggtttgg gggcctgctg
      601 ggtgtgcgcg ccgctgttct gccccgacgt cgtccgcgag acgctcgccc tgccggcgga
      661 ctggcagcct cagggtctca tcacgctcgg ctatccggcc gaggaaaaac aaaagacgcg
      721 ccaccccctg gagtcgcgcg tcctgtatcg ctgattcagt tcggcaatgg ccgggcctag
      781 acgatcacgg cctcgatcac cgggcgcttg cccgtctcat cgtacagata gcgactgaga
      841 gccgattcca cctggccgtc ggttagctcg cgccccgcgg tgtgggcttc gcgcacaacg
      901 cgcgcgatcg tatctccggc acccttcagc agatcatcgg cctctttctc attgacaaag
      961 ccgcgcgtgg tcagcttggg gctgccggcc agctcgccgt ggccgttggt gcgcacgacg
     1021 gcgaaaaaat acccggcctg tgccaaccga tcacgatcac gtacctccgc ccagccgata
     1081 tcgcccacgc cggagccgtc aacgaacaca tagccgcccg gcagccgctc atgcacggtc
     1141 atgctgtagg gagtcaactc aatcggcgtg ccgttctcca cgacggcgat ccgctcctcg
     1201 ggaatgccaa gggccttgcc catctcggcg tgcagcttga gatggcgtaa ctcgccgtgg
     1261 acgggaatga ggaacagcgg ccgaatcagg ttgatgagca gcttcatttc ctcgcggctg
     1321 gcgtgccccg acacgtggac gttggcgatg cgatcataga gcacagtcgc tccgcgtcga
     1381 tagagctggt tgatggtgcg gtagatcaac tcctcgttcc ccggaatcgg gtgcgcggag
     1441 aagataaccg tgtcgccttc ctggatgctg agctggttgt gagtgccgcg tgccaaccga
     1501 cccaggacgg ccgacggctc gccctgcgaa ccggtgacca tgatcaccac ctcgctcggc
     1561 ggcatcttca ccgccacgcc caggtcgaca atgagatcgt caggcaactc cagatagccc
     1621 agcttgcggg cgatctttac tgcgtcgcgc atcgaccggc cggcgatggc catcttgcgg
     1681 ttgaacttga tcgccgccgc cgccgcgatg gtgacgcgag agatcaacga agcaaaggtg
     1741 gcgacgataa tgcgcccctc ggccttgcga aagacatcct caaatccctc cagcagatcc
     1801 atctccgatt ccgtccagcc cgggcggtcg gcgttggtgc tgtcggccat caaagccagc
     1861 acgccgcgct gtgagaactc cgccagtttg gcaaaatcag gcggccaact gtcgatgggc
     1921 gtatggtcga atttgtagtc gccggtatgg acgatcaggc cgaccggcgt ggtaatgccg
     1981 aagccaacgc aatcggggat gctgtgggca acgtgaaacg gctccacctt aaacgggcca
     2041 atctccagca cctcaccggc gcgaaaggag atgagttcca cctcatgcag attcttggcc
     2101 tcgcgcagct
//
LOCUS       DAOMQG010000499         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20551_length_2108_cov_7.666342, whole genome shotgun sequence.
ACCESSION   DAOMQG010000499 DAOMQG010000000
VERSION     DAOMQG010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_2108_cov_7.666342"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1745
                     /locus_tag="PLC06_16475"
     CDS             120..1745
                     /locus_tag="PLC06_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRO92098.1"
                     /translation="MTPPTDCIEFFNPATGAKFGEVRMATAADVARARHEMAAVQPEW
                     AAKSVKERARLVKKLQRVIVDAADEITAVINQDHGKSRLDALHEVFMTVEKIHHYANH
                     APRWLARERVPRGLYFFKRFYTDREPYGVVGIIGPWNYPLDLMVPPIVSALLAGNTVL
                     VKPSEVAAATGVLLEELFSRVPELARYVRFLHGDARVGEAMVRSKPDLLFLTGSVKTG
                     RIVAKMAAENMTPFMYELGGKDPMIVLEDADIAAAARWGVWGAFYSTGQACVSVERVY
                     VHEKVYDAFVAAVLEETAKFSVGYSPDKDNPNWMGPLTFQRQCDIVTAHLDDALAKGA
                     RVLIGGKRTGMFIEPTVLVDVDHSMQLMTEETFGPMMPIMKVRDEAEAIRLANDSDLG
                     LSAYVWSRDLKHAERVAHQLDAGVVNINDVVAHYSVAGLPFGGTKLSGNTRTHGRGEV
                     TQYTRLKAYSFSNPPSAIDPVIWIRYPGNYNLLKAMMGATYGTNMKQRVEPVAEFLSS
                     DKRIRNGVSRAAAGAGLFAAVAGLAAMLLRVRR"
     gene            1882..>2108
                     /locus_tag="PLC06_16480"
     CDS             1882..>2108
                     /locus_tag="PLC06_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92099.1"
                     /translation="MIAEANAPDRTAPVRRWATYREYLATADDTRIVEWADGEIIEYM
                     PPNTVHQKITWFLFRLFSAFAERFDLGLVGAA"
BASE COUNT          400 a          672 c          668 g          368 t
ORIGIN      
        1 agcttacact atttgattca tgcaacaagg ccattacgaa ttacgaatta cgaatgaaga
       61 gcgcgctccg ctcgtatctc gcatctcgta tctcgtatct cgcatagagg tgaaacaaaa
      121 tgactccacc aaccgactgc attgagtttt tcaaccccgc caccggcgcg aaattcggcg
      181 aggtgcgcat ggcgacggcc gccgacgtgg cccgcgcccg gcatgagatg gccgccgtcc
      241 agcccgaatg ggccgcgaag tcggtgaagg agcgcgcgcg gctggtgaag aaactccagc
      301 gggtcatcgt cgacgccgcc gacgagatca ccgccgtcat taaccaggac cacggcaaga
      361 gccgcctcga cgccctgcac gaagtattca tgaccgtgga gaagatccac cactacgcca
      421 atcacgctcc gcgctggctg gcccgcgagc gcgtgccgcg cggcctctac ttcttcaagc
      481 gcttctacac cgaccgcgaa ccctacggcg tggtgggcat catcggcccg tggaactatc
      541 cgctcgatct gatggtcccg ccgattgtgt cggccttgtt ggccggaaac actgtgctgg
      601 tgaagccgtc ggaagtggcg gcggccaccg gcgtgctgct cgaggagctg ttcagccgcg
      661 tgccggagct ggcccgctac gtccgctttc tccacggcga cgcccgcgtg ggcgaggcga
      721 tggtgcgctc caagcccgac ctgctcttcc tgaccggctc ggtgaagacg gggcgcatcg
      781 tggccaaaat ggcggccgaa aacatgaccc cgttcatgta tgagctgggc ggcaaagacc
      841 ccatgatcgt gctggaggac gccgatatcg ccgcggccgc ccggtgggga gtgtgggggg
      901 cgttctattc cacggggcag gcctgcgtct cggtggagcg ggtctacgtc cacgagaagg
      961 tctacgacgc cttcgtggcc gccgtgctgg aagagacggc caaattcagc gtcggctatt
     1021 cccccgacaa ggacaaccct aactggatgg ggccgctcac cttccagcgg caatgcgata
     1081 tcgtcaccgc ccacctcgac gacgccctcg ccaagggggc gcgcgtcctt atcggcggca
     1141 agcggaccgg catgttcatc gagccgacgg tgctggtcga tgtcgatcac tcgatgcaac
     1201 tcatgaccga ggagacgttc ggtccgatga tgccgatcat gaaggtgcgc gacgaggccg
     1261 aggcgatccg cctggccaac gacagcgacc tgggtctgag cgcctacgtg tggagccgcg
     1321 acctgaagca cgccgagcga gtggcgcacc aactggacgc cggggtggtg aacatcaacg
     1381 acgtggtggc ccattactcc gtggccggcc tgccgtttgg cggcaccaag ctgagcggca
     1441 acacgcgcac ccacggccgc ggcgaagtga cccagtacac gcgcctgaag gcctactcgt
     1501 tcagcaaccc gcccagcgcc atcgacccgg tcatctggat tcgctatccg ggcaactaca
     1561 acctgctgaa ggcgatgatg ggcgcgacct atgggacgaa catgaagcag cgcgtggagc
     1621 cggtggccga gttcctcagc agcgacaagc gcatccggaa cggggtatca cgggcggccg
     1681 ccggcgcggg gctgttcgcc gccgtggccg ggttagccgc catgctgctg cgcgtgcggc
     1741 gatagcgaag agggcgaggc agcggcgaac cacatcggtt ctgcggaacc acaaaggttg
     1801 cgccgctgtc ccgcccctgc tgtcccactg acctgctgat ctacagcccc cagctgacct
     1861 gttttctgct atactggaga catgatcgcc gaagctaatg ctcccgaccg gactgctcct
     1921 gttcgccgct gggccacata cagggaatac ctggcgacgg ccgatgacac gcgcattgtc
     1981 gagtgggcag atggggagat tattgaatat atgccgccca atactgttca tcagaagatc
     2041 acctggtttc tattcagact gttcagtgcc tttgctgagc gcttcgattt aggtctggtg
     2101 ggcgcggc
//
LOCUS       DAOMQG010000500         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20711_length_2096_cov_10.624694, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000500 DAOMQG010000000
VERSION     DAOMQG010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20711_length_2096_cov_10.624694"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLC06_16485"
     CDS             <1..699
                     /locus_tag="PLC06_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92100.1"
                     /translation="LLWRVRRPSVRYALAGIVLIGTTLYAVAFVNLYRAEHPWNAASR
                     WIYENVPPGTRILSEQWDDFLPVNLVVDGEQRSRKEYPNAELTWLTFPDAADNEEHLL
                     ANLELLADSEYLTVLSNRVYGVVPRLPERYPLSGQYHQLLFDGTLGYELVWVGSRSPR
                     LWGVELQADTFSWPGLRPPMAVSEYLNRSRSISPGRADESFIVYDQPLTMIFRNTGRL
                     SAEDMMAAFNLADD"
     gene            743..>2096
                     /locus_tag="PLC06_16490"
     CDS             743..>2096
                     /locus_tag="PLC06_16490"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRO92101.1"
                     /translation="MGRRYLFLTVFVAGMSTLAIEFTASRLLQTVYGTSNIVWANVIG
                     LVLLSLTAGYFIGGRVADRYPVASVFYGLVTAAGFAAIFFLLLTGAILRDASAALAVM
                     NVNAVAGSLVLVVLALVVPVTLLGCLSPFAIRLAVQDVSEAGRISGRVYAISTLGSLL
                     GTYLPVLIIIPLAGSRLASVIFGSALLVVGLVGLWRSTGNQAARLGSLILPLLLLPAL
                     AFWLRGGIKTYAGQLYETESAYNYIQVIRRGDCNYLLLNEGQAYHSFYCDGGRVPEIS
                     VWSVMLAAPYFNADPAPPKRMAVVGLAAGTIPKQFTRVFGPIEIDGIELDPAIIDAGR
                     KYFALNEPNIRAITGDGRYELSRLSGPYDVITIDAYKVPYIPWHLTTREFFIEASDRL
                     SERGVLAINVGRVPDDRRLIDAVGATLLSVFPTVHAIDVPGTLNTILVATKQPTTIGD
                     L"
BASE COUNT          350 a          587 c          643 g          516 t
ORIGIN      
        1 cttctctggc gggtgcgccg gccgtctgtc cgttatgccc ttgccgggat cgtcctgatt
       61 gggacaacgc tctatgccgt cgcattcgtc aatctctacc gcgcggagca tccctggaac
      121 gccgcgtcgc gctggatata tgagaatgtt cccccaggga cgaggatcct cagcgagcag
      181 tgggatgatt tcttgcccgt taatctggtc gttgatgggg agcagcgctc gcgcaaggaa
      241 taccccaatg cagagctaac ctggctgacg ttcccggacg cggccgataa cgaggagcat
      301 ctgctggcca atcttgagtt gttggccgat agtgagtatc ttactgttct gtcgaatcgt
      361 gtctatggcg tggtaccgcg actgccggaa cggtatcccc tgtcgggtca gtatcaccag
      421 cttcttttcg acggcacgct cggctatgag ctggtttggg taggcagtcg atcgccccgg
      481 ctatggggcg ttgagttgca ggcggatacg ttttcgtggc ctggcttgcg gccgccaatg
      541 gcggtgagcg agtatctgaa ccggtctcgc agcatttctc ctgggcgcgc cgacgagagc
      601 tttatcgtgt atgatcagcc gttaacgatg atatttcgca atacgggaag attatcggct
      661 gaagatatga tggccgcttt caatttggcc gatgattaac cctgcaatca gcaacagagg
      721 ccaggagaag aaaacataca agatgggccg ccgctatttg tttctgactg ttttcgtggc
      781 tggaatgagc acactggcga ttgagttcac cgccagccgg ttgctgcaga ccgtctatgg
      841 aacgtcaaac attgtctggg ccaacgtgat tggcctggtg ctgctttcgc tgactgccgg
      901 ctatttcatc ggcggtcgtg tggcagaccg ttatccggtg gcttctgtgt tttatgggct
      961 agtgaccgcg gccgggttcg ccgccatctt ctttcttctg ctgaccgggg ccatcctgcg
     1021 cgacgcttcc gcggccctgg cggtcatgaa cgtcaatgcg gtggccggtt cactggtgct
     1081 ggtcgttctc gcgctggttg tgccggtgac gcttctcggc tgcctctccc cgtttgccat
     1141 tcgcctggcc gtgcaggatg tcagcgaggc ggggcggatc agcggccgtg tctacgcgat
     1201 ctcgacgctg ggcagcttgt tgggcacgta cttgcctgtt ttgattatta tcccgctcgc
     1261 ggggtcgcgg ctggcctcgg tgatcttcgg ttcggctcta ctagttgtgg gtctggtggg
     1321 tttgtggcgc tccacgggta atcaagcagc gcggttaggg tcactcatac tgccgcttct
     1381 gttgttgccg gccctggctt tctggctgcg tggcggcatt aagacctatg ccggtcaact
     1441 ctatgagacc gagtcggctt ataactacat tcaggtgatt cgccggggcg attgcaatta
     1501 cctgctgctg aacgaggggc aggcgtacca ttcgttctat tgcgatggcg gccgtgtccc
     1561 ggagatctcc gtatggagcg tcatgctggc cgctccctac ttcaacgccg atcccgcccc
     1621 gccgaagcgg atggcggtag ttgggctggc cgcgggcacg atccccaagc agttcactcg
     1681 cgtctttggc cccattgaga tagacggcat tgagcttgac ccggccatta tcgatgccgg
     1741 ccgcaagtat ttcgcgctca acgagccgaa tattcgagcg ataaccggcg acggacgata
     1801 cgaattgtcc cgattatctg ggccttatga cgtcataacg atcgatgcct ataaggtgcc
     1861 ctatatcccc tggcatctga caacccgcga gtttttcatc gaggcgagcg accgtttgag
     1921 cgagcgtggc gtgttggcga tcaatgtcgg ccgcgtgccc gatgatcggc ggctgattga
     1981 tgccgtcggc gcgacgctgc tgtccgtctt ccccaccgtt cacgctatcg acgtccccgg
     2041 aacattgaat acaatcctgg tggcgacaaa gcagccgacg acgattggcg atctgc
//
LOCUS       DAOMQG010000501         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20742_length_2094_cov_5.230015, whole genome shotgun sequence.
ACCESSION   DAOMQG010000501 DAOMQG010000000
VERSION     DAOMQG010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20742_length_2094_cov_5.230015"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PLC06_16495"
     CDS             complement(<1..1034)
                     /locus_tag="PLC06_16495"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRO92102.1"
                     /translation="MNSERLERLTEQIVANGLDGLALVPGPNMVYVSGIHAHLSERPI
                     VLFLPADDDPAIIIPSLEAMKARAAGIPEDRIFDWDDAEGYHGAFQQACARLELADYM
                     LGVEALHMRVLELQTLQRYAPGLQIAHAEPALSALRSVKDADEVAAMERAIAVAEKAL
                     KRIIPRIKIGLSERQIAAMLTNELLASGAESIAFGPIVAGGPNSAIPHAVPGDRAIRS
                     GDLLVIDWGVYVDGYPSDITRTLAVGEIDDELRHIWDVVNAANAAARKAVRPGVTGQA
                     VDRVARDVIEAAGYGEYFIHRTGHGLGLEIHEPPDMSPVNTQPIAAGNVFTIEPGIYL
                     PGRGGVRIED"
     gene            complement(1057..1557)
                     /locus_tag="PLC06_16500"
     CDS             complement(1057..1557)
                     /locus_tag="PLC06_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92103.1"
                     /translation="MTQFQLRINDETREFNVTRQGDQLVVTSDGQAADVRILHRDESG
                     LLIEIRREGSVPRRVRLAGARSGDKRQLWIDGHTLTAERVRRSAATGGPDSGSLSASI
                     PAIVSQVLVAPGDEVAAGDKLILLESMKMVIPIQAPRDGRVARVLCAPGESVPAGVPL
                     VELESE"
     gene            complement(1749..>2094)
                     /locus_tag="PLC06_16505"
     CDS             complement(1749..>2094)
                     /locus_tag="PLC06_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRO92104.1"
                     /translation="VHGDYGFDNVMCRDGRVTSVLDWAEARLGDPLYDVAWLDYWSRL
                     PLGDLWHEQARRDAAGFEARMVCYLLHIGLASMVIAARLRDERDYRRVRDRTVSALSL
                     ARRRSTDWPEEG"
BASE COUNT          331 a          676 c          714 g          373 t
ORIGIN      
        1 ttgtcctcga tgcgcacgcc gccgcgaccg ggcagataga tgcccggctc gatggtgaat
       61 acattgccgg cagcgatggg ctgcgtgttg accggactca tatccggcgg ctcgtggatc
      121 tccagtccca gcccgtggcc ggtgcggtgg atgaagtact cgccataacc ggcggcctca
      181 atgacgtcgc gggccacccg atcgaccgcc tgaccggtga cgccggggcg caccgccttc
      241 cgcgccgccg cgttggccgc gttcaccacg tcccagatgt ggcgcaattc gtcgtcgatc
      301 tcccccacgg ccagggtgcg ggtgatgtcg gacgggtagc cgtcgacata gacgccccag
      361 tcgatgacca gcaggtcgcc gctgcgaatg gcccggtcgc ccggcacggc gtgggggatg
      421 gcgctgtttg ggccgccggc gacgattggc ccgaaggcga tgctctccgc ccccgacgcc
      481 agcagctcat tggtcagcat ggcggcgatc tgtcgctcgc tgaggccgat cttgatgcgc
      541 gggatgatgc gcttgagcgc tttctcggcc acggcgatgg ccctctccat ggccgccacc
      601 tcgtcggcgt ccttcaccga gcgcagcgcc gacagcgccg gctcggcgtg ggcgatttgc
      661 aggccggggg catagcgctg cagcgtttgc agctccagca cgcgcatatg gagcgcctcg
      721 acccccagca tgtagtcggc cagttcgaga cgggcgcagg cttgctggaa cgcgccatga
      781 tacccttcgg cgtcgtccca atcgaagatg cgatcttcgg ggatgcccgc cgcgcgcgct
      841 ttcatggcct ccagcgaggg gatgatgatg gccgggtcgt cgtcggcggg gagaaagagg
      901 acaatcggcc gctcgctcaa atgggcgtgg atgccgctga cgtaaaccat attcggcccc
      961 ggcaccagcg ccagcccatc caggccattg gcgacgatct gctcggtgag gcgttccaga
     1021 cgttcagaat tcatggatgt tctccgttca ctatatttat tcgctctcca gttccaccag
     1081 cggcaccccg gccggcaccg attcccccgg cgcgcagagc acccgtgcca ctcggccgtc
     1141 gcgcggggcc tgaatgggga tgaccatctt cattgattcc agcaggatga gcttgtcacc
     1201 cgccgccacc tcgtcgccgg gagccacgag cacctgcgag acgatggccg ggatcgaagc
     1261 cgacaacgag ccgctgtcgg ggccgcccgt ggccgccgag cgtcgcaccc gttcggccgt
     1321 cagcgtgtgg ccgtcgatcc acagctgccg cttgtcgccc gaccgcgcgc cggccagccg
     1381 cacgcggcgg gggacgctgc cttcccggcg gatctcgatg aggaggcccg actcgtcgcg
     1441 atggagaatg cggacgtcgg ccgcctggcc gtcgctcgtg acgaccagtt ggtcgccttg
     1501 gcgcgttaca ttgaactcgc gcgtttcgtc gttgatgcgt agctggaatt gggtcattga
     1561 tcggtattgt atatcaaaag atttcaggag gagaacccgc ggatttggcg gattgaagtc
     1621 agtgggtcag taggtcagtg ggtcagtgga tcagtggatc agtaggtcag tggatcagta
     1681 ggtcagtggg tcagtttgga ctgaatactg ccctactgaa tactgcccta ctgaatactg
     1741 cccactgttc acccttcttc cggccaatcg gtcgatcgcc ggcgggcgag ggagagggcg
     1801 gagacggtgc ggtcgcggac gcggcggtag tcgcgctcgt cgcgcaggcg ggcggcgatg
     1861 accatgctcg ccaggccgat gtggagcaga tagcagacca tccgtgcctc gaagccggcc
     1921 gcgtcgcgcc gcgcttgctc gtgccagagg tcgcccagcg gcaggcggct ccagtagtcg
     1981 agccaggcca cgtcatagag tgggtcgccg aggcgcgctt cggcccagtc gagcacgctg
     2041 gtgacgcggc cgtcgcggca catgacattg tcgaagccgt agtcgccgtg gacg
//
LOCUS       DAOMQG010000502         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20750_length_2093_cov_7.886163, whole genome shotgun sequence.
ACCESSION   DAOMQG010000502 DAOMQG010000000
VERSION     DAOMQG010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20750_length_2093_cov_7.886163"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLC06_16510"
     CDS             complement(<1..602)
                     /locus_tag="PLC06_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92105.1"
                     /translation="MESGNEWETVPPIGRVLRASTAGFVIGCQVQQLSEPSFGCLVKA
                     QPVDDREAVFGLIYNMDVADDPLVRRIVLAENPREEVINDQRQNRLLPIEMSVLAVGY
                     CLNGRFFHGLPPRPPLNLDPVHLCHDAAELVRFTDRLGYLRLILRAADMGVPVDQLLV
                     AHVQDVFRLRGHDVEWATGVINEVIELLRSDYDTLVPVLEA"
     gene            complement(701..1981)
                     /locus_tag="PLC06_16515"
     CDS             complement(701..1981)
                     /locus_tag="PLC06_16515"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HRO92106.1"
                     /translation="MTLELDKLTPDLEKMAQAAAANRQAQLNRAKQLRQLVAERATDW
                     PGIRAGLQKAEEEADPKYFRAAAPLSEDEPLDTPIPPPAPPEQATIIATDGSQIMPDR
                     HAAWLYYLINIGVIVYNHGSGRGHGDGRAPHVFTSPRIFYPNVEDDVTEDEPGFDQSQ
                     VTIARDRAEIETLADFADAWRAASGPVLALLDQRLLYWPMVGERGSAEPIVAHWTEAM
                     RRIHETGALLAGYIDRPGKRSVVTMLQTLLDEPDMDWKALGKRPVGNELTDAGLYATL
                     LGPGDRSPIFVDISPANKRFAEESPDIEVCFFYLNAGSSNIIVGDDLDPLGEARAIAR
                     VDIPRWVAEDKAAVAAVHGLIYDQCRIQRNYPYVLTRADELAVVGRDDETNLSVMIDL
                     AMQRAGVESSVTAKKIGKDFARGGRRPHRMGRYW"
BASE COUNT          355 a          665 c          691 g          382 t
ORIGIN      
        1 gcctcgagca ccggcaccag cgtgtcgtaa tcgctgcgca acaactcgat gacctcgttg
       61 atcaccccgg tcgcccactc cacgtcatgg ccgcgcagcc ggaagacgtc ctgcacgtgg
      121 gccacgagca actggtcgac cggcacaccc atatcggccg cgcgcaggat gagccgcagg
      181 tagccgagcc gatcggtgaa gcgaaccagt tcggccgcgt cgtggcacag gtgaaccggg
      241 tcgaggttaa gcggcgggcg cggcggcagg ccatgaaaga agcggccgtt gaggcagtag
      301 cccacggcca gcacgctcat ctcgatcggc agcagccggt tctggcgctg gtcgttgatg
      361 acctcctcgc gcgggttttc ggccaggacg attcgccgca ccagcggatc atcggccacg
      421 tccatgttgt agatgaggcc gaagacggcc tcgcgatcgt ccaccggctg ggccttcacc
      481 aggcagccga acgacggctc cgacagctgc tgcacctggc agccgatgac gaacccggcc
      541 gtactcgccc gcaggacgcg gccgattggc ggtactgttt cccactcatt gcctgattcc
      601 aatggtgtcc tccctgactg catcattcgt agaaaccgag tttcttccaa gaaactcggt
      661 ttctatgtgc gtcattcgtc actcgtcact tccccacatc ttaccaatac cgccccatcc
      721 ggtgcggtct gcggccgcca cgggcgaaat ccttgccgat tttcttggcc gtcacgctgc
      781 tttcaacgcc ggcccgctgc atggccagat cgatcatcac gctgagattg gtctcgtcgt
      841 cgcgaccgac cacggccaac tcatcggccc gcgtcagcac gtaggggtag ttgcgctgga
      901 tgcggcactg atcatagatc aggccgtgga cggcggccac ggcggccttg tcctcggcca
      961 cccagcgcgg gatatccacc cgggcgatgg ctcgcgcctc acccaacggg tcaaggtcgt
     1021 cccctacaat gatattactg gacccggcgt tgaggtagaa gaagcaaacc tcgatgtcgg
     1081 gcgattcctc ggcgaagcgc ttgttggcgg gggagatgtc gacgaagatc gggctgcgat
     1141 cgccggggcc gagcagagta gcatacagcc cggcgtcggt cagttcgtta cccaccgggc
     1201 gcttgcccag ggccttccag tccatatccg gttcgtcgag gagcgtctga agcatggtca
     1261 cgactgagcg cttgcccggc cggtcgatgt agccggccag cagcgcgccg gtctcatgga
     1321 tacggcgcat ggcctccgtc cagtgggcga cgatcggctc ggcgctgccg cgctcgccga
     1381 ccatcggcca gtagagcagc cgctggtcga gcaaggccag cacggggccg gaagcggcgc
     1441 gccaggcatc ggcgaaatcg gccagcgtct cgatctcggc ccggtcgcgg gcgatggtca
     1501 cctggctctg gtcgaagccc ggctcgtctt cggtcacgtc gtcctcgacg ttggggtaga
     1561 agattcgcgg cgaggtgaaa acgtgggggg cgcggccgtc cccatggccc cggccggagc
     1621 cgtggttata gacgatgacg ccgatgttga tcagatagta gagccaggcc gcgtgacggt
     1681 cgggcatgat ctgcgagccg tcggtggcga tgatcgtcgc ctgctccggc ggcgcgggcg
     1741 gcgggatcgg cgtgtccagc ggctcatcct ccgacagagg cgcggcggcg cggaagtatt
     1801 tggggtcggc ttcctcttcg gccttttgca gcccggcgcg aatgccgggc cagtcggtgg
     1861 cccgctcggc caccagttgc cgcaactgct tggcgcgatt taactgggcc tgccggttgg
     1921 cggccgcggc ctgcgccatc ttttcaaggt cgggggtcag tttgtcaagc tccagcgtca
     1981 tggggcggta gtgtagcacg gatgagctag agatacgaat gcaagatcaa gatacggtag
     2041 attgcgctgt ttggtcaata ggctgcgacg tgcattgaca taacgtcatt agt
//
LOCUS       DAOMQG010000503         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20797_length_2089_cov_10.842675, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000503 DAOMQG010000000
VERSION     DAOMQG010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20797_length_2089_cov_10.842675"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..508)
                     /locus_tag="PLC06_16520"
     CDS             complement(50..508)
                     /locus_tag="PLC06_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92107.1"
                     /translation="MNETPYPASPITVDETGHEPLVPIRYIVIGVIVLLVVGVAFAAL
                     LAYLAINFADTIVIVRDIFIIALGIMSCLSGIVLILLLISIIRLINMLEFELKPILLK
                     TNDTLGTIRGTTAFMSDNVMRPVTTASSYMAGVRRGVQTLFGDPRRNLGK"
     gene            complement(564..839)
                     /locus_tag="PLC06_16525"
     CDS             complement(564..839)
                     /locus_tag="PLC06_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92108.1"
                     /translation="MITIPGSASGLVLGFLLATIYGGLFHLVFGGPIRRILVYLLAAW
                     VGFFVGQFIGDFFNLELLKLGKIHLVPASIGAWGALLAAWWLVGQDS"
     gene            complement(836..2002)
                     /gene="mnmA"
                     /locus_tag="PLC06_16530"
     CDS             complement(836..2002)
                     /gene="mnmA"
                     /locus_tag="PLC06_16530"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRO92109.1"
                     /translation="MSILTSTPNQTDKGRVVVAMSGGVDSSVAAALLVEQGYEVIGMM
                     MRLWSEASVGPATATNRCCTPDQMADARRVADKLGIPFYVADVRQQFRQAIVQFFLDE
                     HEAGRTPNPCVECNRQIRFTYLLERALAMDADFLATGHYARIESTSNGYVLMQGLDLN
                     KDQSYVLHTLRQDQLAHVLFPISSYTKDEVREIARRFGLSVASKRESQDLCFLSDGDY
                     RRFIRDNSIQIHQPGPILDLDGHELGRHQGLPFYTIGQRKGLGIAAAEPLFVLRKDTA
                     RNALIVGPRDSLGARELVAEGVNWISGIIPTSPITAQVKIRYKAAPIPATVTPLGGER
                     AHVRFDEPAFGVTPGQAAVFYDGSVCLGGGLIINDDAREEEGHLISGIAEGIRG"
BASE COUNT          476 a          601 c          583 g          429 t
ORIGIN      
        1 gagccgagtt cgctttcgtt attatccatt tcaatttctc ctaaaactgc tatttaccca
       61 ggtttcgtcg tgggtcacca aaaagggtct ggacgccgcg ccgcacgccc gccatgtagc
      121 tgctggcggt ggttaccggg cgcattacat tgtcgctcat aaaagccgtg gtcccgcgga
      181 tggtgcccaa cgtgtcgttt gtcttcagaa ggatcggctt cagttcgaat tcaagcatgt
      241 tgatcaggcg gatgatgctg atcagcagca ggatcaggac gatgccggag aggcaggaca
      301 tgatgcccag cgcaatgata aaaatatccc ggacgatgac gatcgtatca gcaaaattga
      361 tcgccagata ggccaggagt gcagcaaaag ccacccccac gaccaacagg acaattacgc
      421 caatgacgat atacctgatt gggaccaatg gttcatggcc ggtttcatca acggttatag
      481 gcgaggccgg atatggtgtt tcattcatcg gataaacccc aacttacaag tatatcagga
      541 atccataacg gtctcaattg tgctcatgaa tcctggccca ccagccacca ggcggccaga
      601 agcgcccccc aggccccgat actcgcgggg acgagatgga tcttgcccag ctttaatagc
      661 tcaaggttaa aaaagtcccc gatgaactgc ccgacaaaga aacccaccca ggccgcgagg
      721 agatagacca ggattcgcct gattggtccg ccgaaaacca ggtgaaacag gccgccataa
      781 atggtggcca acaaaaagcc caataccaat cccgacgctg aacctggaat ggttatcatc
      841 cgcgtatgcc ctccgcaatg ccggatatga gatgaccttc ttcttcacgc gcgtcatcgt
      901 tgatgatcaa gccgccacca aggcacaccg acccatcata gaaaacggcc gcctgccccg
      961 gtgtgacgcc gaacgccggc tcgtcgaatc gcacatgggc tcgctcgcca cccagcggcg
     1021 tcacggttgc cgggataggc gcagccttgt atctgatctt cacctgggct gtaatgggag
     1081 atgttgggat gatgccgctg atccagttca ccccttcggc cacgagttcg cgcgcgccca
     1141 aagagtcgcg cggccccacg atgagcgcat tacgcgctgt gtcttttcgc aggacgaata
     1201 gaggttcggc cgcggcgatc cccaggcctt tgcgctgacc gatggtgtag aaaggcaagc
     1261 cctgatgccg gcccaactcg tggccgtcca gatcgagaat ggggccgggt tggtgaattt
     1321 gaatgctgtt gtcgcgaatg aaccggcgat aatcgccatc gctcagaaag cacagatcct
     1381 ggctttctcg cttcgatgcg acagaaaggc cgaaccggcg ggcaatctcg cgcacttcat
     1441 ctttggtgta gctgctaatg gggaatagta cgtgagccaa ctgatcctgg cgaagcgtgt
     1501 gcaacacgta ggattgatcc ttgttcaagt ccagcccttg cataaggacg tagccattcg
     1561 acgtggattc tatccgcgca taatggccgg ttgccagaaa gtcggcatcc atggccaatg
     1621 cccgttcaag cagataggtg aaccggatct ggcgattgca ttcgacgcac gggttaggcg
     1681 tcctgccggc ttcgtgttcg tccaggaaaa actggacgat ggcttgccga aactgttggc
     1741 gcacatcggc gacgtagaag gggataccca gtttgtcggc cacgcgccgg gcgtcagcca
     1801 tttgatccgg cgtgcaacag cgattggttg cggtagccgg gccgacgctc gcctcgctcc
     1861 acagacgcat catcatgccg atgacttcgt aaccctgctc gacgagcaat gcggccgcga
     1921 ctgaactatc gacccccccg ctcatggcga cgacgactcg gcctttatca gtctggttgg
     1981 gggtggatgt caggatgctc atattcaata agccgtcacg aaggcggctt ccagagcggc
     2041 cagatcccgc gctatggcag tcagcgtctt caccgtgtat tcgatctcg
//
LOCUS       DAOMQG010000504         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20798_length_2089_cov_7.215339, whole genome shotgun sequence.
ACCESSION   DAOMQG010000504 DAOMQG010000000
VERSION     DAOMQG010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20798_length_2089_cov_7.215339"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1075
                     /locus_tag="PLC06_16535"
     CDS             68..1075
                     /locus_tag="PLC06_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92110.1"
                     /translation="MNYTTLIGDSWRLVWRRKSLIALGSLSVLASILAYVALYIGFIA
                     FFLSSPSLLIRLMGMPLTEMPEASGGPLGYIAMVLGALAWLLLWLVGLSARGGMIAAV
                     DAHEDGRSLGAGAAFGRGWSRALTLAAMAIVVFLPVMILYLITIPLLFSRFPDLMNAG
                     EAEIMSALMTTQALSCSLSLLIYGLMALLQFIYAFAYRGVVLGGLGVMASIRHGWRVL
                     RGNAGDILPLALIFGGIWFGLIVLWYIAFFVIYLILLFGLALGSGDVSLPLTIAAAVG
                     FFILLLVTLLAGGAQSAWRSAAFTSGYRYWIAEKKEPAAVEASPMESADDAPFTDPPT
                     P"
     gene            1215..>2089
                     /locus_tag="PLC06_16540"
     CDS             1215..>2089
                     /locus_tag="PLC06_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92111.1"
                     /translation="MDYVNTIKRGFALTWEHKFLWVLGFLAALGSGGSFSGSNYSSSG
                     GNMAGVEQWMTPERMAALTGGLVVFGCIGFIVGIILWLVALSARGGLIGAVAQMEQGA
                     AKPSFGSAFRLGWSKVWRLAGMTIALYIVPFILFMVFLVGFFAVAGGLAYAGSSMEDM
                     SAIGAGIGGLALVFVCLICLMIPFLITLSLIYPFAFRGIVLRDMGIRQSISHGWQTLK
                     AHLGDIILLGLAFFLVGIFVMIIAAAILVPVALVVGVPIIALSGSDATVITGVLAVLG
                     IVIGMVVFALVSAITT"
BASE COUNT          301 a          628 c          696 g          464 t
ORIGIN      
        1 gcgccgtcgc cggttatctc gcccattcgt ttgtagcagg ctatagtaat agcaattgga
       61 gggcagcatg aattacacga cgttgattgg cgacagttgg cggttggtgt ggcggcgcaa
      121 atccctgatc gcgctgggat ccctcagcgt gctggcctcg atattggcgt atgtcgccct
      181 ctatatcggc ttcatcgcct tttttctctc ctcgccgtcc ctgctcatca ggctgatggg
      241 catgccactc acggagatgc cggaggcaag cggcgggccg ctcggctaca tcgccatggt
      301 gctgggcgcg ctggcgtggc tgctgctgtg gcttgtgggg ctttcggcgc ggggcgggat
      361 gatcgcggcg gtggacgccc acgaggacgg ccgttcgctg ggggccggcg cggcgttcgg
      421 ccgcggttgg tcgcgggcgc tcacgctggc ggcaatggct atcgtggttt ttctgccggt
      481 catgatcctc tacctcatca ccatcccgtt gctattttcg cgctttcccg acctgatgaa
      541 tgccggcgag gcggagatca tgagcgcact catgaccaca caggcgctct cctgctccct
      601 ctcgttgctc atctacggcc tcatggcgct gctgcaattc atctacgcct tcgcctatcg
      661 gggggtggtg ctgggcgggc tgggggtcat ggcgtccatt cgtcacggct ggcgggtgct
      721 gcgcggcaat gccggcgata ttctgccact ggcgctgatc ttcggcggaa tctggtttgg
      781 gttgatcgtc ctgtggtaca tcgctttctt cgtcatctac ttaatcctgc tattcgggct
      841 ggcgctgggg agtggggacg tgtctttgcc tctgacaatc gcggcggccg tggggttctt
      901 catcctgctg ttggtcacgt tgctggccgg cggggcgcaa tcggcgtggc gatcggccgc
      961 gttcacgtcg ggataccggt actggatcgc cgaaaagaag gagccggcgg ccgttgaggc
     1021 aagccccatg gaaagcgccg atgatgcacc cttcaccgac ccgcccaccc cataaacagg
     1081 actttttacc taacttaagc ccacaggctg aaatttgggc aaccccaggc gcaaccgcgc
     1141 gtaaagattg ttagcaacag ataagcgaat ccggcgcggc ccgtggtggc cgggccattc
     1201 aaggagtaaa tatcatggat tatgtcaata caatcaaacg cgggttcgcc ctcacctggg
     1261 agcacaaatt cctgtgggtg ctgggctttc tggcggcgct gggcagcggc ggcagcttct
     1321 cgggatccaa ctattcgagc agcggcggca acatggccgg cgtcgagcaa tggatgacgc
     1381 cggagcgcat ggccgcgctg accggcggcc tggtcgtctt cggctgcatc ggattcatcg
     1441 tgggcatcat cctgtggcta gtggccctca gcgcgcgcgg cggcctgatc ggcgcggtgg
     1501 cccagatgga gcagggcgcg gccaagccga gcttcggcag cgccttccgc ctgggctgga
     1561 gcaaggtctg gcggctggcg gggatgacga tcgccctcta catcgtgccg ttcatcctgt
     1621 tcatggtgtt tctggtcggc ttcttcgccg tggccggcgg gctggcctat gcgggcagca
     1681 gcatggaaga catgtcggcc atcggcgcgg gcatcggcgg gctggcgctg gtcttcgtct
     1741 gcctgatatg tctgatgatc ccgttcctca tcaccctgag cctgatctat cccttcgcct
     1801 tccgcggcat cgtcctgcgt gatatgggca tccgccagag catcagccac ggctggcaga
     1861 cgctgaaggc ccacctgggg gacatcatcc tgctggggct ggcgttcttc ctggtcggca
     1921 tcttcgtcat gatcatcgcc gcggccattc tggtgccggt ggcgctggtc gtgggcgtgc
     1981 ccataatcgc cctcagcggg tcggacgcga cggtcatcac cggcgtgctg gcggtgctgg
     2041 gcatcgtcat cgggatggtg gtcttcgcgc tggtgtcggc catcaccac
//
LOCUS       DAOMQG010000505         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20820_length_2087_cov_9.093012, whole genome shotgun sequence.
ACCESSION   DAOMQG010000505 DAOMQG010000000
VERSION     DAOMQG010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20820_length_2087_cov_9.093012"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLC06_16545"
     CDS             complement(<1..349)
                     /locus_tag="PLC06_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRO92112.1"
                     /translation="MDELILTRQRDALFEIILNRPDKRNAIHLDMFRAFDAAVSQANR
                     IPGLRAVLIRGAGESFSAGIDVSALLGLAQTFGPHWMQRMRSITDELQGILTRLERLE
                     LPTIALLHGHCLGL"
     gene            complement(486..854)
                     /locus_tag="PLC06_16550"
     CDS             complement(486..854)
                     /locus_tag="PLC06_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRO92113.1"
                     /translation="MSVIGNIIWLIFGGFITGLAYILGGLLLCLTIIGIPFGLKAIRV
                     GMATLTPFGKAVIPDERGDGCLPLMLNIIWILLFGWEIALAHLVSAGFLAITIIGIPF
                     AKQHFKLIPVAFAPFSFRLA"
     gene            1040..1873
                     /gene="trpA"
                     /locus_tag="PLC06_16555"
     CDS             1040..1873
                     /gene="trpA"
                     /locus_tag="PLC06_16555"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRO92114.1"
                     /translation="MNEAKRAMQAAATGREQIAAAFAAAKQRGRSAALMPYYTLGYPN
                     RAASLDIIAAIAADSDLLELGVPFSDPLADGPTIQHSTQVALEQGTTLDDCLEMVREL
                     RHRGVHTPILLFGYYNPFLAYGLDRLAGAARAAGAQGFIVPDLPPEEAADMGAAAARE
                     GLAYIYFLAPTSNAHRIEVVTGQANGFIYLVSVTGVTGARQSIETNLAEFIGRVRSHT
                     TAPLAVGFGISTPEQAASVGGMADGVIVGSALINAVDQAKTNKPAAAAAFVRSLRTAL
                     E"
     gene            1883..>2087
                     /locus_tag="PLC06_16560"
     CDS             1883..>2087
                     /locus_tag="PLC06_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRO92115.1"
                     /translation="MTNRLANETSPYLRQHANNPVDWYPWGEEALQRARDEDKPILLS
                     IGYAACHWCHVMAHESFENPATAE"
BASE COUNT          477 a          632 c          593 g          385 t
ORIGIN      
        1 ctaaccccag acaatggcca tggagcaggg cgatggttgg caactccagc cgctcaagcc
       61 gggtcaggat accctgaagc tcatcggtga tcgagcgcat gcgttgcatc caatgggggc
      121 cgaacgtctg ggccagccca aggagcgcgc tgacgtcgat tcctgccgag aagctctcgc
      181 ccgcgccccg gataagaaca gcccgcagac cagggatgcg gttggcctgt gagacggccg
      241 catcgaacgc ccggaacatg tccagatgga tggcgttacg cttgtccggc cgattgagga
      301 tgatctcgaa gagcgcgtct cgttgccggg tcaggatcag ttcgtccata tgcctatctc
      361 ccaaaataaa aaggatctcc ctgatgatat agaccgaagt ctacgtcaat cggggagatc
      421 cgtgtaactt gtgtcaagcc tgatcgcgac gatcagggct ttgttctttg gcctatcgct
      481 ctcgtctagg ccagccggaa gctgaacggc gcgaaggcta ctggaatcag cttgaaatgc
      541 tgcttggcga acgggatgcc gatgatagtg atggccagaa aaccggcgct caccagatgg
      601 gccagggcga tctcccagcc gaacagcaga atccagataa tgttaagcat caatggcaga
      661 cacccatcgc cgcgttcgtc ggggatgacg gccttgccga aaggcgttag cgtggccata
      721 cccacacgaa tcgccttcaa gccgaacggg atgccgataa tagtcaagca tagcaacaaa
      781 ccgcccagaa tgtatgccag cccggtgata aatccaccaa aaattaacca gataatgttt
      841 ccaattacgc tcattttccc tctgaatatc cgcgacttta agtgaaaatg ttgcgcggaa
      901 tcataatact atacgtgaga gttgggcatt ggtttcaccg acgataagca tgtccggtga
      961 ttagccgatt tcatgactcg cccatcgtgt actataatga tcctaacgcg ctcaactgga
     1021 atgatgctgg aggatgacga tgaacgaagc caaaagagcc atgcaagccg cagccaccgg
     1081 ccgagaacag atcgccgccg cgtttgccgc agcgaagcaa agaggccgct cggccgcgct
     1141 gatgccctat tacacgcttg gctatcccaa ccgcgctgct tcgctcgata tcatcgccgc
     1201 catcgccgcc gacagcgatc tgctcgaact tggcgtaccc ttcagcgatc cgctggccga
     1261 cgggccgacc atccagcaca gcacgcaggt ggcgctggaa caaggcacga cgctcgatga
     1321 ttgcctggaa atggtgcgcg agttgcgcca tcgtggcgtc cacacaccga tcctgctctt
     1381 cggctattac aacccgttcc tggcctacgg gctggatagg ttggccggcg cagcgcgcgc
     1441 ggccggggct caaggtttca tcgtccccga tctgccaccc gaagaagcgg ccgacatggg
     1501 agcggccgcc gctcgcgaag ggctggccta tatctatttc ctggccccaa ccagcaacgc
     1561 ccaccgcatc gaggttgtga ccggccaagc caacggcttc atctatcttg tcagcgtcac
     1621 cggcgtaacc ggcgcgcggc aaagcatcga aacaaatctg gccgaattca tcggccgcgt
     1681 gcgcagccat acaaccgcgc cactggccgt ggggtttggt atcagtaccc cggaacaggc
     1741 ggcttcggtt ggcggcatgg ctgacggggt catcgtcggc agcgcgctga tcaacgccgt
     1801 cgatcaagca aaaacgaaca aaccggcagc cgcggccgct tttgtccgtt cactgcggac
     1861 agcattggaa taggtgaatt cgatgaccaa cagactggcg aacgaaacca gcccctacct
     1921 gcgccaacac gccaataacc ccgtggactg gtatccgtgg ggcgaggaag cgctgcagcg
     1981 tgcccgcgac gaagacaagc cgatcctgct cagcatcggt tacgccgcct gtcattggtg
     2041 ccatgtgatg gcccacgagt ccttcgaaaa cccggctacg gccgaat
//
LOCUS       DAOMQG010000506         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20856_length_2084_cov_12.979793, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000506 DAOMQG010000000
VERSION     DAOMQG010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20856_length_2084_cov_12.979793"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..574
                     /locus_tag="PLC06_16565"
     CDS             143..574
                     /locus_tag="PLC06_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRO92116.1"
                     /translation="MLTVNDLMTIIPNTVTPQTSLRHVIELMKTQYCRQLPVLDNGKL
                     VGIITDRDVRLVMNSPLVLHGRWQDEEILDNITAENCMTPDPMTIGPDTPAYQAANML
                     SIYKFGALPVVDNGVLVGIITVTDFLNYFADQHPETIDQQG"
     gene            594..1526
                     /locus_tag="PLC06_16570"
     CDS             594..1526
                     /locus_tag="PLC06_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRO92117.1"
                     /translation="MAPFRNILVPLDGSELAEKALEPANQIAMAMVQRGKEAPVVKLT
                     LLRALSPMALLAADPYLYDEMMRMSEDEARAYLNHIASTLTASAVVIETRIVSGSPAD
                     AIVQYAEDNKADLIVMSSHGRTGSRRWVYGSVAEKVMHHAPCATVIIRAHVDVSMFQN
                     KKILVPLDGSELAERALEPAMALARAVNSNVYLLRVVAAREPVPESMTPTGERVAAAL
                     DAADLEERGEAEAYLQSVYSRYENTHLFFDVQTTAGDVADRIVTYADTNQIDLIVISS
                     HGRSGLGRWLHGSVAEKVLRGATCATLIMRGQNE"
     gene            1707..>2084
                     /locus_tag="PLC06_16575"
     CDS             1707..>2084
                     /locus_tag="PLC06_16575"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRO92118.1"
                     /translation="MNLPRPLQPEALYNALDPAEFPFETTAELEDGLEIIGQQRAIDA
                     IRFGIGIRHQGYNLFALGPNGAGRQTTANRFLRARAANEPVPDDWCYVYNFEHPHKPR
                     AHRLPPGRANIFRDDMKKLAEELF"
BASE COUNT          490 a          556 c          566 g          472 t
ORIGIN      
        1 ttcttaaagt agcgctgaaa aatcccgatg aatgtcatcc atcaaaggtg acattcgcca
       61 taatgcgcgc gtgaacataa agggttacac tgaaagcagg agataacctt acccgggttt
      121 gccactgatg ggagaaatcc atatgctgac tgtaaatgat ttaatgacga ttatccccaa
      181 tacggttaca ccacaaacaa gtctgaggca cgtcattgaa ctgatgaaga cgcagtattg
      241 tcggcagttg cccgtattgg acaatggcaa actggttggc atcatcaccg atcgcgatgt
      301 gcggctcgtg atgaactcac cgcttgtcct gcacggccgc tggcaagatg aagaaatatt
      361 ggataacatc acagccgaga attgtatgac gcccgaccca atgacgatcg ggccggacac
      421 tcccgcatat caggcagcca atatgttgag catttacaaa tttggcgctc ttccggtggt
      481 cgataacggc gttctggttg gcatcatcac cgtcactgat ttcctcaact atttcgccga
      541 tcaacacccg gaaacaattg atcaacaggg ctaacccgcc caaggaggcg aacatggcac
      601 cgttccgtaa tatattggtc cctcttgacg gatccgaact ggctgagaag gccctcgagc
      661 cggccaatca gatcgcgatg gccatggttc aacgcggcaa ggaggcaccc gttgtaaaac
      721 tgactttgct ccgggccttg tcgccgatgg cgctgctggc cgcggatccc tatctatacg
      781 atgaaatgat gcgaatgagc gaggatgagg cgcgagccta ccttaatcat atagcatcca
      841 cactgacggc gagcgcggtc gtcattgaaa ccaggatcgt gagcggctct ccggccgacg
      901 ccatcgtcca atatgctgaa gacaataagg ccgatctcat cgtgatgtcc agtcatgggc
      961 gcaccggcag tcggcgctgg gtttatggta gcgttgccga gaaggtgatg catcatgccc
     1021 cttgcgctac tgtgatcatt cgtgcccacg tcgatgtatc catgttccag aacaagaaga
     1081 ttcttgtccc cctcgacggt tccgagttgg cggagcgcgc gcttgaaccg gctatggcgt
     1141 tggcacgagc ggtcaactca aacgtgtatt tgttgcgcgt tgtagctgcc cgggaaccgg
     1201 tgcctgagtc catgacgccc accggggagc gggttgcggc ggcgcttgac gcggccgacc
     1261 tggaagagag aggcgaagcg gaagcctatc tgcaatcggt ttattcccgg tacgagaaca
     1321 cgcatctatt cttcgatgta cagacaacag ccggagatgt ggccgacagg atcgtaacct
     1381 atgccgatac gaatcaaatt gacctgattg tgatatccag ccatggacgt tccggattgg
     1441 gtcgctggct gcacggcagt gtggccgaga aggtgttgcg tggggcaact tgtgcgacat
     1501 tgattatgcg cggacaaaac gaataagcga catcgtcgca aggcagacga ttcttattat
     1561 tgctatgtgg cagtggatcg gctacagtcc gattcactgc catttttctt tcagtggata
     1621 gcctggcgcc cgattattcc gctcctgccg ccccatccct tcgggactat caccaggtta
     1681 ttgctcgcga aattatgggg agatatatga atctaccgcg acccctccaa cccgaagcac
     1741 tctacaatgc tttagacccg gctgaatttc catttgagac aacagcggaa ctggaagacg
     1801 gcctggaaat catcggtcag caacgagcca ttgatgctat tcgcttcggc atcggtatcc
     1861 ggcatcaggg gtacaacctt ttcgcgctgg ggccaaatgg ggctgggcgg caaacgactg
     1921 ccaatcgctt tctccgggca cgtgccgcga acgagcctgt ccctgatgat tggtgctacg
     1981 tctataactt tgagcatcct cacaagcctc gtgcccaccg cttgccgcct ggtcgcgcta
     2041 atattttccg cgatgatatg aaaaaattgg ccgaggagtt gttc
//
LOCUS       DAOMQG010000507         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_20919_length_2080_cov_6.188148, whole genome shotgun sequence.
ACCESSION   DAOMQG010000507 DAOMQG010000000
VERSION     DAOMQG010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20919_length_2080_cov_6.188148"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLC06_16580"
     CDS             <1..267
                     /locus_tag="PLC06_16580"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRO92119.1"
                     /translation="AGVTAALFLIGRIVLAIFLALSNTGSIYGAAGSLIILLLWIYYS
                     AQLLLYGAEFTRLYALRHGQAIRPNRLSRMMAAEQRADLSTDDH"
     gene            399..1388
                     /gene="plsX"
                     /locus_tag="PLC06_16585"
     CDS             399..1388
                     /gene="plsX"
                     /locus_tag="PLC06_16585"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRO92120.1"
                     /translation="MRIVVDAMGSDERPGPDVAGAVMAARKFGDTIILAGDKPLVEAE
                     LAAHDTKGLSIEIVHAAEEIGMKDSPSASAHNKRESSIHVGLRLVREGAADAFVSAGN
                     TGAILATATLRQTGLGRIPGVLRPALGVVFPIDTKPMLIDNGANADAKPEYLLQFGMM
                     GSIYMERMHGINRPRVALISNGEEEGKGNLLIKETIPLMEASGLNYVGNIEPKEFVRG
                     AADVAVVDGFTGNVMMKTAEAVASYLIEAIRRNIMAGTLTKIGGALARPAFKRVRDEF
                     NPDEVGGAPLMGVNGVVIIAHGRSNAYAVQQAIGQARRVVDGGIVAAIAEGVK"
     gene            1488..>2080
                     /locus_tag="PLC06_16590"
     CDS             1488..>2080
                     /locus_tag="PLC06_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRO92121.1"
                     /translation="MSNETLDARGRPRVVVTGMGMLSPLGNTVEESWENMMAGRSGIT
                     RITQFDASELPTQIAGEVKNFVARDYMDFKEARRMSRASQLAVAAARMAVADAGLPEQ
                     VPDPDRVGVLVGTAAGGMERAFAEIDAFNERGFRAVNPFGMTMFLCNMPTHHVSLTTG
                     STGPLSTIVAACATGTQTIGEGAEFIRRGAADMMLAGG"
BASE COUNT          402 a          620 c          672 g          386 t
ORIGIN      
        1 gcaggagtga cggcggcgtt gttcctcatc ggccggatcg ttctggcgat cttcctggct
       61 ctcagcaaca ccggatcgat ctatggcgcg gcgggatcgc tcatcatcct gcttttgtgg
      121 atttattact cggcccaact cctgctctat ggcgcggagt tcacccggct ctatgccctg
      181 cgccatggtc aggccatccg ccccaaccgt ctgtcgcgga tgatggccgc cgagcagaga
      241 gccgacctgt cgaccgatga ccattgaccg gcgaccgttg caagaaatcc atgcagaatg
      301 tagagaacgg ggtgacggac ggtagcgcga ccctttttct gcagccgggc cgattgtgat
      361 agaatcctcg ctattatcct gcaaaagagt atacaagtat gcgtatagta gtcgatgcaa
      421 tgggtagtga tgagcgtccc ggccccgacg tggccggagc ggtgatggcc gcgcgcaagt
      481 ttggtgacac gatcatcctg gcgggtgaca agccgctggt cgaggcggag ttggccgccc
      541 atgacaccaa ggggctgagc atcgagatcg tccacgccgc cgaggagatc ggcatgaagg
      601 attcgccaag cgcgtcggcc cataacaagc gcgagtcgtc gattcatgtc ggtctgcggc
      661 tggtgcgcga gggcgcggcc gatgccttcg tcagtgcggg caacaccgga gccatcctgg
      721 ccacggccac cttgcgccag accggactcg gccgcattcc gggcgtgctg cgcccggccc
      781 tgggcgtcgt cttccccatc gacaccaaac ccatgctgat cgacaacggg gccaacgccg
      841 acgccaagcc ggaatatctg ctgcaattcg gcatgatggg cagcatctat atggagcgca
      901 tgcacggcat caatcggccg cgtgtggcgc tcatctccaa tggcgaggaa gagggcaagg
      961 gcaatctgct tatcaaggaa acgattccgc tcatggaagc cagcggcctg aactatgtcg
     1021 gcaacatcga gccgaaggag tttgtgcgcg gcgcggccga tgtggctgtt gtcgacggct
     1081 tcaccggcaa cgtcatgatg aagacggccg aggcagtcgc gagctatctg atcgaagcca
     1141 ttcgcagaaa cattatggcc ggcacgctga ccaagattgg cggcgcgttg gcccgcccgg
     1201 cctttaagcg cgtgcgcgac gagttcaacc ctgatgaggt cggcggcgcg ccacttatgg
     1261 gcgtcaacgg ggttgtaatt atcgcccacg gccgctcgaa cgcctatgcc gtccagcagg
     1321 ccatcggcca ggcgcggcgg gtggttgatg gcggcatcgt tgccgcgatt gccgagggcg
     1381 tgaagtagag aggaattcaa tgcaatgaca cgagaaggca aaggtgtaag atattaactt
     1441 ctttccttac gccttacact ggagatgttt cgtctaaaag gaactttatg agcaatgaga
     1501 ctttggatgc gcgcgggcga ccgcgcgttg ttgtaaccgg gatgggcatg ctcagcccgc
     1561 tcggcaatac cgtcgaagag agctgggaga acatgatggc cggacgctcc ggcatcaccc
     1621 gtatcaccca attcgacgcg agcgagctac ccacgcaaat cgccggcgag gtgaaaaact
     1681 tcgtcgcccg tgactatatg gatttcaagg aggcccgacg tatgtcgcgc gcctcgcaac
     1741 tggctgtggc cgcggcgcga atggcggtgg ccgatgccgg gttgcccgag caggtgcccg
     1801 accccgaccg cgttggtgtg cttgtgggca cggccgccgg cggcatggag cgcgcgtttg
     1861 ccgagatcga cgccttcaac gagcgcggct ttcgcgctgt caatccgttc ggcatgacga
     1921 tgttcctgtg caacatgccc acgcaccacg tgagcctgac gaccggctcg accggcccgc
     1981 tcagtaccat tgtggccgct tgcgccacag ggacgcagac catcggcgag ggggccgagt
     2041 tcattcgccg cggcgcggcc gacatgatgc tcgccggcgg
//
LOCUS       DAOMQG010000508         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21076_length_2067_cov_6.635686, whole genome shotgun sequence.
ACCESSION   DAOMQG010000508 DAOMQG010000000
VERSION     DAOMQG010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21076_length_2067_cov_6.635686"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..523
                     /locus_tag="PLC06_16595"
     CDS             74..523
                     /locus_tag="PLC06_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92122.1"
                     /translation="MPTNPRPTIKKSNLRLLTADTKFYIDYSWWDEGHLDLRTYLLSR
                     LNIGTDLGPELPAERIDLVDSKTGEVRQVDAFQYLVRNYFSRHDNELSMQGSVVDAVF
                     SVLLANGNEPMTAAELAERIQRTPELITKTFGGAQIYNGIRPLFDDD"
     gene            750..823
                     /locus_tag="PLC06_16600"
     tRNA            750..823
                     /locus_tag="PLC06_16600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Arg,seq:tct)
     gene            852..>2067
                     /locus_tag="PLC06_16605"
     CDS             852..>2067
                     /locus_tag="PLC06_16605"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRO92123.1"
                     /translation="MTIKVFSVAEMVAAEKAADAAGHSYARMMETAGQRVAEAILQRH
                     DVEGRQLLILVGPGNNGGDGLVAGRYLAEAGAMVAFYLFKSRDALEDDNLARVEEMGL
                     EVLPADYDQRYRVLRHRMRTADIIIDALLGTGVSRPIAGPLANLMEQIAAGLAERQGD
                     AAEQESLVSVAAPPIASDEAPSPGDRPSRPVVVAVDCPSGLNCDTGELDPLTIPADLT
                     ITFAGPKRGHFKFPGAAACGELVVADIDIDPQLPAVAGVPVELATAAMARRLMPKRPA
                     DGHKGTFGTALIAAGSIRYWGAPALAARGAFRAGVGLVTLAVPQRLRPALAGQFPEAI
                     YPLVTDTDTLGVSTANLLLASLSDYSALLVGPGLGDAASFVGALLDGLGGATDDPPPP
                     LIMDADGLNMLAT"
BASE COUNT          374 a          624 c          655 g          414 t
ORIGIN      
        1 aagccgttgg cgatcattac tggcagtatg agcgctcgtc ctagggcgcg tatccgaaga
       61 ggagatagag tctttgccta ctaatccacg gcccacgatc aagaaatcga accttcgcct
      121 cttgacggcc gataccaagt tctatatcga ctatagctgg tgggatgagg gtcatttgga
      181 cctgcgcacg tatttgcttt cgcgcctgaa tatcggcacc gatctcggcc ccgaattgcc
      241 tgcggaacgg atcgatctgg tggattcgaa gacgggcgaa gtgcgacagg tcgatgcctt
      301 tcaatacctg gtgcgcaact acttcagccg ccacgacaac gaattgtcga tgcaaggctc
      361 ggtggtcgat gccgttttca gcgtgctgtt ggccaacggc aatgagccga tgacggccgc
      421 cgaactcgcc gagcggattc agcgcacgcc tgagctaatc accaagacct tcggcggggc
      481 gcagatctac aacggcatac ggccactgtt cgacgacgat taacccatcc gctaccgcgc
      541 atgaactgga gctgtagagc aagcctccgg cttgcctctt cagttgtgga acatgccgtc
      601 accctgacct cctgcaagcc gccggcttgc actacaccgg tgcactgtat acttgtcgag
      661 catctgaaca tatgagccgc ctggccatat gagaggcgtt gacggacacc ccgcatctct
      721 ttagaattga atcgatacag acatttgctg cccccgtagc tcaattggac agagcgtcgg
      781 ccttctaagc cgaatgttgc aggttcgagc cctgccgggg gcactgattc accgctggaa
      841 agttggcccc tatgaccata aaagtattta gcgtcgccga gatggtggcg gccgaaaaag
      901 cggccgacgc cgccggacac tcctatgccc gcatgatgga aacggccggt caacgggtcg
      961 ccgaagcgat cttgcagcgt catgatgtgg aaggcaggca attgctcatc ctggtggggc
     1021 cgggcaataa cggcggcgac ggcttggtgg ccgggcgcta tctggctgag gccggcgcga
     1081 tggtggcttt ctatcttttc aaatctcgcg atgcgctgga ggacgataac ctggctcgtg
     1141 tcgaggagat ggggctggag gtgttgcccg ccgactacga ccagcgctat cgtgttttgc
     1201 gtcatcggat gcggacggcc gatatcatca tcgacgcctt gctcggcacc ggcgtgtctc
     1261 gcccgatagc cgggccgctg gcgaacctca tggagcagat cgccgccgga ctggccgaac
     1321 ggcagggaga tgcggccgaa caggagagcc tggtcagcgt agccgccccg ccgatcgctt
     1381 ccgacgaagc gccgtccccc ggcgatcgac cttcgcggcc ggttgtcgtg gcggtggatt
     1441 gccccagcgg tctcaactgt gacaccggcg aactggaccc gttgaccatt cccgctgatc
     1501 tgacgatcac tttcgccggg ccaaaacgtg gccacttcaa atttcccggc gcggccgcgt
     1561 gtggggagct ggttgtcgcc gatatcgaca tcgaccccca attgcccgct gttgccggcg
     1621 tgcccgtcga gttggctacg gccgccatgg ctcgccggct gatgccgaag cgtcccgccg
     1681 atggtcacaa gggcaccttc ggcacggcgc tcatcgccgc gggcagcatc cgctattggg
     1741 gtgcgccggc gctggccgcg cgtggggctt ttcgcgccgg ggtggggctg gtgacgctgg
     1801 ccgtgccgca gcggttgcgt ccggcactgg ccgggcagtt ccccgaggcg atctacccgc
     1861 tggtgaccga taccgataca ctgggcgtat cgacggccaa tttgctgctg gcctcgctga
     1921 gtgactatag cgccttgctg gtcgggccgg ggctgggtga tgcggcgtcg tttgtcggcg
     1981 cattgctgga tggattaggc ggcgcaactg atgacccgcc tccaccgctg atcatggatg
     2041 ccgacgggtt gaatatgctc gcgacaa
//
LOCUS       DAOMQG010000509         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21136_length_2062_cov_10.257598, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000509 DAOMQG010000000
VERSION     DAOMQG010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21136_length_2062_cov_10.257598"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLC06_16610"
     CDS             <1..471
                     /locus_tag="PLC06_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92124.1"
                     /translation="ALLGAIKLPPIKPMTAEPPRLSGATFDGQVRLLGFDTPESAIAG
                     ETLPITLYWEAVEPNGRDYTVFIHLLNDAGELVAQSDGQPGAGTYPTSIWAGGERVVD
                     EHILALPLELPAGDYILFVGLYDHESGTRLPALGPDGRHNPDDAVNIATIRIDE"
     gene            501..1616
                     /locus_tag="PLC06_16615"
     CDS             501..1616
                     /locus_tag="PLC06_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92125.1"
                     /translation="MKLLNLRYLIYGFLVIAVIAAIVLLAAIPATSRALPNAAGSSAG
                     TPSAYALLPMIRYDASPTPVPTPGDPPFDAAAALYITPHEHLLASTYNTGSFRVKNIS
                     RGSEQLVELRIDLTTAVFPDMVFDPFGAAGDQVAKDLTVDVQSGMGFEGHKYEGLHAG
                     GFDVMVLKFRSFDRGDEFFFSVDVDPTSIAGVGAPGPGESGSVGGLELVGATITATFN
                     DGTVLTNQVSRMDDPGNGGVTHSGAVAVMRPGLPDRPVLGIPGVGSPAVVDVANQTVR
                     VNGPVGRPYILMVIEGGLFTEGVPNGGFDLDPFESNTAITVREYRGKIGLDGTADVPV
                     TLSKSMPEGGINIITAVFDNHYGVNGRVAVPLILQLD"
     gene            1740..>2062
                     /locus_tag="PLC06_16620"
     CDS             1740..>2062
                     /locus_tag="PLC06_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92126.1"
                     /translation="MGVSSNGLWRRLWLALPLIGITLLLMGGHGAGATEVDLKNRAGD
                     TERVFMPHVAKVKGPPILKLVPFVYGFDNITITAITNAGDERLFVTIREGKIWIVNPA
                     GQILPT"
BASE COUNT          384 a          561 c          658 g          459 t
ORIGIN      
        1 gccttgcttg gtgcaattaa gttgccgccg atcaagccga tgacggctga accgcctcgt
       61 ctttccggcg cgaccttcga tggtcaagtc cgcttgcttg gcttcgacac gccggagagc
      121 gcgattgcgg gcgagacgct gcccatcacc ctgtattggg aggcggtcga gccgaacgga
      181 agggattaca ccgtttttat tcatttgttg aacgacgcag gcgagctggt ggcccagtcc
      241 gacggccaac cgggcgcggg aacctatcca acctcgattt gggccggcgg cgaacgcgtt
      301 gtggatgagc atattttggc cctgccgctc gagttgccgg cgggggacta catactgttt
      361 gttgggttgt atgatcacga gagcgggaca cggttgcccg cgcttggccc ggatggacgt
      421 cacaaccctg acgacgcggt gaacatcgcg acaatacgga ttgatgaata accatccgga
      481 tttgggagtc cgagactgtt atgaagttgt tgaacctgcg ctatctgata tatggctttt
      541 tggttatcgc ggtcattgcg gcgatcgtgc tgttggcggc gatcccggcc acgagccgcg
      601 ccctgccgaa tgccgcgggg agttctgccg gcacgccgtc ggcctatgct ctgctgccga
      661 tgatccgcta tgacgcctcg ccgacgcccg tgccgacgcc gggtgatccg cctttcgacg
      721 ccgcggccgc actctatatc acgccccatg agcacctgtt agccagcacc tataacaccg
      781 gctcttttcg tgtgaagaac atttcgcgcg gtagtgaaca gctggttgag ttacgtattg
      841 acctgacaac ggccgtgttt cccgacatgg tcttcgatcc gttcggggcg gccggcgatc
      901 aggtggccaa ggatctcacc gttgatgttc aatcgggcat gggttttgag ggtcacaagt
      961 atgaaggcct gcacgccggc ggcttcgatg tgatggttct gaagttccgc agctttgatc
     1021 gtggcgacga attctttttc tccgtcgatg tagaccccac gagtatcgcc ggggttggcg
     1081 cgccggggcc gggggagtcc ggcagcgtcg gcgggctgga gctggtcggg gcgacgatta
     1141 cagccacctt caacgacggc acagtgctga ccaatcaggt gtcgcgcatg gatgatcccg
     1201 gtaacggtgg ggttacccac agcggcgcgg tggccgtcat gcggccgggt ctgcccgata
     1261 ggcctgtgct gggcatccct ggcgtcggca gcccggcggt tgtggacgtg gccaatcaga
     1321 ctgtgcgcgt gaacggcccg gttggtcgtc cctatatcct gatggtgatc gagggcgggc
     1381 tattcaccga gggcgtaccc aatggcggct ttgatctgga tccgtttgag agcaacacgg
     1441 cgataaccgt ccgggaatac agggggaaga tcggcctcga tgggacggcc gacgtgccgg
     1501 ttaccctctc caaatccatg cccgaagggg ggatcaatat cattacggcc gttttcgata
     1561 accattatgg cgtcaatggt cgggtggccg tgcccctgat cctgcagctt gattaatcgg
     1621 atgcctgcgc gcctcatttg tactaaaatg cacgaacgat agttgaattt cactggctcc
     1681 tgcctgtgtc gtcgcaacag cgaggggaca ggcagaacga gcggacaggc agggatagaa
     1741 tgggagtttc atcaaacggg ctatggcgcc ggctgtggct ggcgttgccg cttataggca
     1801 ttactttatt actcatgggt ggtcacgggg ccggagctac cgaggttgat ttaaagaatc
     1861 gggcagggga tacggagcgt gttttcatgc cgcatgtggc caaagtgaaa gggccgccaa
     1921 tactgaaact ggttcctttc gtctatggct ttgacaatat cacgatcacc gctatcacca
     1981 atgccggaga cgagcgtttg ttcgtgacta ttcgcgaggg caaaatctgg atcgttaacc
     2041 ctgccgggca gatattgcca ac
//
LOCUS       DAOMQG010000510         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21137_length_2062_cov_7.369706, whole genome shotgun sequence.
ACCESSION   DAOMQG010000510 DAOMQG010000000
VERSION     DAOMQG010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21137_length_2062_cov_7.369706"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLC06_16625"
     CDS             complement(<1..812)
                     /locus_tag="PLC06_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRO92127.1"
                     /translation="MNFKPFNQPAPQLGNQYDDDRVLRSYLRRVLPPDMLAAVEPSLR
                     EMGELAGGELYQLQLDDRLNMPVLTQWDAWGERIDHIEVTAVWKRAERLAAEFGIVGA
                     AYEPEYGPFARPWQFALAYLFHPSTDVYTCPLAMTDGAARTLLLSGNRELIDRAVPRL
                     TSRDPAQFWTSGQWMTESTGGSDVGLSETVAVLDREENWHLHGRKWFTSATTSQMALT
                     LARPEGNRPGGRGLAMFYLELRDDDGRLNHITINRLKDKLGTRKVPTAELMLG"
     gene            complement(890..2059)
                     /locus_tag="PLC06_16630"
     CDS             complement(890..2059)
                     /locus_tag="PLC06_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRO92128.1"
                     /translation="MPGPLASLRILDFTTLLPGPFATMALADMGAAVVRVESPTRPDM
                     VRFLPPYDGDTSAWHGVLNRNKRSLALDLKRPDAADVIRRLVTTGGYNIVIEQFRPGV
                     MERLGAGYEDLRAANPALIYCALTGYGQTGPLRDRAGHDNNYLALSGALSYSGRRETG
                     PPPLGIQVADIGGGALGALVGLLAAVVHRAATGEGQFVDISMLDMMLAWQAHLISHYL
                     VAGETPEPEEMALNGGRVYDLYETADGRRLSVGSLEPKFWEGFCAAIDRPDLIAPGLS
                     LDPDEQARVKAEVKAAILTRPLAEWAAVFAPLDVCVEPVLTVPEALAQPHVAARELVI
                     DVPRLTGGAQRQIASPWRFSVGTTGYRHTGAALGAHTAEVLREAGFTETEIENYL"
BASE COUNT          332 a          667 c          723 g          340 t
ORIGIN      
        1 ccgagcatca gctcggccgt cggcaccttg cgcgtaccca gcttgtcctt cagccggttg
       61 atggtgatgt gattgaggcg gccgtcgtcg tcgcgcagct ccagatagaa catggccagg
      121 ccacggccgc cgggccggtt gccctccggc cgggccagcg tcagggccat ctgcgaggtg
      181 gtggcgctgg tgaaccattt tcggccgtgg agatgccagt tctcttcccg atccagcacg
      241 gcgacagtct ccgacaggcc cacgtcggag ccaccggtgg actcggtcat ccattggccg
      301 ctggtccaga attgggccgg atcgcggctg gtcaggcgag ggacggcccg gtcgatcagc
      361 tcgcggttac cgctgagcag cagcgtgcgg gccgcgccgt cggtcatggc cagcgggcag
      421 gtgtagacat cggtcgaggg atggaacagg taggccaggg caaactgcca ggggcgggcg
      481 aaggggccat actccggctc ataggccgcg ccgacgatgc cgaactcggc cgccagccgc
      541 tcggcgcgct tccagacggc cgtcacctcg atgtgatcga tgcgctcgcc ccaggcatcc
      601 cactgggtca gcaccggcat attcagccgg tcgtcgagtt gcaattgata aagctcgccg
      661 ccggccagct cgcccatctc gcgcagcgaa ggctcgacgg cggccagcat atcgggcggc
      721 agcacgcggc gcaggtatga gcgcagcacg cggtcgtcat cgtactggtt acccaactgt
      781 ggggcaggtt ggttaaacgg tttgaaattc attttgttcc cccatttgcg gtgtgggtgt
      841 gagttttata actcgctgtc tccatgtggc gccggttggg aaaccggcgc tacaggtagt
      901 tctcaatttc ggtttcagtg aatcccgctt cgcgcagcac ttcggccgta tgcgctccca
      961 gggccgcgcc cgtgtgacgg tagccggtcg taccaacgga gaagcgccag ggagaagcaa
     1021 tctgtcgctg cgccccgccg gtgagtcggg gcacatcgat caccagctcg cgcgcggcca
     1081 cgtgaggctg ggccaacgct tcgggcacgg tcagcaccgg ctcaacgcac acatcgagcg
     1141 gtgcgaacac ggcggcccac tcggccagcg gccgggtcag aatagccgcc ttcacctcgg
     1201 ccttgacccg ggcttgctcg tcaggatcga ggctgaggcc gggagcgatc agatcgggcc
     1261 ggtcgatggc ggcgcagaat ccctcccaga atttcggctc caggctgccg acggagagcc
     1321 ggcggccatc ggccgtctcg tagagatcat agacccggcc gccgttgagg gccatctcct
     1381 ccggctcggg cgtctccccg gcgacgaggt aatggctgat gaggtgggct tgccaggcga
     1441 gcatcatgtc cagcatgctg atgtcgacga actgcccttc gccggtcgcg gcgcgatgga
     1501 cgacggccgc cagcagcccg accagcgccc ccagcgcccc accgcctatg tcggccacct
     1561 ggatgcccag gggcggcggg ccggtctcgc gacggccgct gtagctgagc gcgccggaca
     1621 gggccagata gttattgtcg tggccggcgc ggtcgcgcag cggaccggtc tggccatagc
     1681 cggtcagggc acagtagatg agcgccgggt tggccgcgcg caggtcttcg tagccggcgc
     1741 ccagccgctc catgacgccc ggccggaact gttcgatgac gatgttgtag ccgccggtcg
     1801 tcaccagccg gcggatcacg tcggccgcgt cggggcgctt cagatcgaga gccagcgagc
     1861 gcttgttgcg gttgaggacg ccatgccagg cggatgtgtc gccgtcatac ggcggcagga
     1921 agcgcaccat gtccggtcgg gtgggcgatt cgacgcgcac gacggccgcg cccatgtcgg
     1981 ccaacgccat cgtggcgaaa gggccgggga gcaaggtggt gaagtcaagt atcctgagcg
     2041 aagccagcgg gccgggcatg ct
//
LOCUS       DAOMQG010000511         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21179_length_2058_cov_8.758362, whole genome shotgun sequence.
ACCESSION   DAOMQG010000511 DAOMQG010000000
VERSION     DAOMQG010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21179_length_2058_cov_8.758362"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLC06_16635"
     CDS             <1..338
                     /locus_tag="PLC06_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO92129.1"
                     /translation="TSDAFNAVAEPQRRRIIDLLALGERSVNDIVEALDMRQPQVSKH
                     LGVLREVGLVNVRDEGRRRLYSLNSEALRPIYEWVTPYERFWRERYERLEELLDRLQR
                     QTSGDEPEQ"
     gene            390..866
                     /locus_tag="PLC06_16640"
     CDS             390..866
                     /locus_tag="PLC06_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004032058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRO92130.1"
                     /translation="MSKPEIIVEPGSQEIVTKMTFNAPRALIFKAYTDPDLIPQWYGP
                     RYLTTVVDVLDARAGGSWRFLNYDPEGNVYAFHGVYHDVVAPERIVQTFEYEGMPGHV
                     VMETAVFEDLGDGKTRVVTQSVFQSVEDRDGMAASGMEVGVNEGYERMNELMATLQ"
     gene            997..1920
                     /locus_tag="PLC06_16645"
     CDS             997..1920
                     /locus_tag="PLC06_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92131.1"
                     /translation="MRTTTSWRRPFIEAAAIVLLVLSLYMYWFAQADRYIVFLYGHTA
                     PGIPLTEPFDAITSSRYWMAGLVAAGVVMILYTGVNWIGGRIAARRGSSFGTPPWWRV
                     WALSAPGLIAGIPAITMTANSPVLPLSLAVASAAAALVGLALALLPGRWAADCPDDLL
                     WLMLDGMGLVPVLLLVRVIELPGRGLSVSPAVVWFFAIGSIIGGALWLALLSVLRRWG
                     DRPSPGAMAIFLAGLGLSYTLMPLVHYLTGGPPGFRYISNSANFFAVNPLLQLVAFGA
                     AAGLAVAAARFRDRLSRSPLTDDQTSRPAAE"
     gene            complement(1941..>2058)
                     /locus_tag="PLC06_16650"
     CDS             complement(1941..>2058)
                     /locus_tag="PLC06_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRO92132.1"
                     /translation="LVLVAVFAFIFLGERPGAREWFGIALVATGVLVLAIKR"
BASE COUNT          352 a          569 c          692 g          445 t
ORIGIN      
        1 ctacatcgga tgctttcaac gccgtcgccg aaccgcagcg gcggcggatc atcgatctgc
       61 tggcattggg tgagcgatcg gtcaacgata tcgtggaggc gctcgacatg cgtcagccgc
      121 aggtgtcgaa gcatctgggc gtgttgcgtg aagtgggtct ggtgaacgtg cgcgacgagg
      181 gccggcgccg gctttactcg ctgaacagcg aggcgttgcg ccccatttat gaatgggtga
      241 ccccctacga acgcttctgg cgcgagcggt acgagcggtt ggaggagctg ctcgaccggt
      301 tgcagaggca aacatccggc gatgaaccgg agcaataaac ccaaagtcgt catatgacga
      361 cgtcaatatc aaacatagga gaacacatca tgtcgaagcc ggagattatc gttgaaccgg
      421 gaagccagga gattgtcacg aagatgacct ttaatgcgcc acgcgcgctc atcttcaagg
      481 catacaccga tcctgacctc atcccgcagt ggtatgggcc tcgctatcta acgactgtgg
      541 ttgacgtgct ggatgcgcgt gcgggcggta gttggcgctt cctcaattac gatcccgaag
      601 ggaatgtcta cgcctttcat ggagtctatc acgatgtggt tgccccggag cggatcgtgc
      661 agacgttcga atatgagggg atgccgggcc atgtggtgat ggagacggcc gttttcgagg
      721 accttggcga cgggaagacg cgcgtggtga cgcagtcggt tttccaatcg gtcgaagacc
      781 gcgacgggat ggccgcgtcg ggcatggaag tgggcgtcaa tgaaggttat gagcggatga
      841 atgaactgat ggcgacgctg cagtaggcgc agtcgctgag ttgacaggtt gaggcaagag
      901 ccatgggcga accgctcatg gctctttttt gtgtctcatt tgccggatcg cgatcaatgg
      961 ctgacgaacc gcgataatgc ggctatgatc aggggtatgc gtaccacaac aagttggcga
     1021 cggccgttca tcgaggcagc cgctattgtg ctgcttgttt tgagtctata tatgtattgg
     1081 ttcgctcagg ccgaccgata tatcgtcttt ctctatggcc acactgcgcc cggcatcccg
     1141 ctgaccgaac cgttcgacgc catcacgtcc agtcgctact ggatggccgg gttggtggcg
     1201 gcgggggtgg tcatgatcct ctataccggc gtaaactgga tcggcggccg gattgcggcg
     1261 cgaagaggct cctcgtttgg cactccgccc tggtggcggg tgtgggcgct gtccgcaccg
     1321 ggactgattg ccggcatccc ggcgatcacg atgactgcca attcgccggt gctgccgctc
     1381 tctctggccg tggctagcgc ggcggcggcg ctggtcgggc tggctttggc tttgttaccg
     1441 ggccgatggg cggccgactg tcccgacgat ttgctctggc tgatgctgga cggcatggga
     1501 ttggtgcccg ttttgctgct cgtgcgagtg attgaattac cggggcgtgg cctgtcggtt
     1561 tcgcccgcgg tggtctggtt ctttgccatc ggcagcatta tcggcggggc gctttggctg
     1621 gcgctgctca gcgtgctgcg gcggtggggc gatcggccgt cgccgggagc gatggctatt
     1681 tttctggccg gactggggct gagctataca ctgatgccgc tggttcacta tctgaccggc
     1741 gggccgcccg gattccgcta catctccaac tcggccaact tcttcgccgt taacccgttg
     1801 ttgcagttgg tggcgtttgg tgcggcggcg gggttggccg tggccgccgc gcgatttcgg
     1861 gatcgcttgt cgcgcagccc gctgaccgac gatcagacgt ctcgcccggc ggccgaataa
     1921 gcggcattgt ctctgtttgc ctaacgcttg atcgccagca ccagcacgcc cgtggcgacg
     1981 agcgcgatgc cgaaccattc gcgcgcgccg ggccgttcgc cgagaaagat gaaggcgaac
     2041 accgccacca gcaccagg
//
LOCUS       DAOMQG010000512         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21285_length_2050_cov_8.518797, whole genome shotgun sequence.
ACCESSION   DAOMQG010000512 DAOMQG010000000
VERSION     DAOMQG010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21285_length_2050_cov_8.518797"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLC06_16655"
     CDS             <1..434
                     /locus_tag="PLC06_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRO92133.1"
                     /translation="GRLNKRLENESFILMGPGRWGSVNLDLGVHVTYGDIFNTKVLIE
                     IAIAHDGHIPELSYGTHFFQDLVEAGIHSLALHLGGDSREADFKWDFFNDTANHLAEV
                     LPEDAALDRYLKVIDLDRLPGSPRLNVLMNGANEEAIGYLS"
     gene            448..>2050
                     /locus_tag="PLC06_16660"
     CDS             448..>2050
                     /locus_tag="PLC06_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRO92134.1"
                     /translation="MSNFRLRTTTPTIDIYIKLAQYPILADRLRERMRQELFGRGIVN
                     PDKFEQEVHELCIASQKREGLGDPYYEEDSQTWQMRMERIRDYHTDAYFADNVGSVRL
                     EALIAELLNSRSSAPDTVAISFNPEIAPWEMLFRQGEAFERMPPDELERYRHHLEEIK
                     VVLIKRLMSDQLPFIGVAKRVLTIDDLRRIYSGLIGTGKIGGKAAGMYLAWRILQEVD
                     ERYGRAAINIPDSYFIGTDVLYEFFLMNKLEHYMNQKYRSVREIRSEYPRIVEDFLAS
                     SLPDYLVVQIREVVESLGDSPLIVRSSSLLEDNFGFSFAGKYNSYFCANQGTPEENLA
                     QVLEAIRRVYASSVNPDALLYRKKHDLIDYDERMAILLQRVTGRWHGRYFYPDMAGVG
                     FSENPFCWTPEIRRGEGFLRLVAGMGTRAVDRVAQDYPRLIPLSHPELRPETTVEGQR
                     NFAQRYMEAVDREQNGIVTVPVREALLSNSTTLPLVASIDTGTSMLPIQPDTTIGADD
                     HIVMTFDGLTQDATFIEFMREILQHL"
BASE COUNT          433 a          610 c          569 g          438 t
ORIGIN      
        1 tcgggcggct caacaagcga ctggaaaatg aatcgttcat cctcatgggg ccggggcgtt
       61 ggggcagcgt caatctcgac ttgggcgtgc atgtgaccta tggcgacatc tttaacacca
      121 aagtcctcat tgagatcgcc attgcccacg acggccatat acctgaactc tcgtatggca
      181 cccacttctt ccaggacctg gttgaggccg gcattcactc gttggccttg catttgggcg
      241 gcgactctcg cgaggccgac tttaaatggg atttcttcaa cgacacggcc aaccacctgg
      301 cggaagttct tcccgaagat gccgccctcg atcgttatct caaagtgatt gacctggatc
      361 gcctgccggg tagtccgcgg ctgaacgtgc tcatgaatgg cgcgaatgaa gaagccatcg
      421 gctatttgag ctaacacaag gggtatcatg tccaactttc gcctgcgcac gacaacacct
      481 accatcgaca tctacatcaa gttggcccag tatcccatcc tggccgaccg gctgcgcgaa
      541 cgcatgcgac aggagctttt cggccgcggc atcgtcaatc cggacaagtt tgagcaagag
      601 gtgcatgaac tgtgcattgc ctcccagaag cgagaggggc tgggcgatcc gtattacgag
      661 gaggattcgc aaacctggca aatgcgcatg gagcggatcc gcgattacca caccgacgcc
      721 tatttcgcgg acaatgtcgg cagtgtccgg ctcgaggcgt tgatcgccga actgctcaat
      781 agtcgctctt cagccccgga tacggtggcg atctccttca atccggaaat tgcaccatgg
      841 gaaatgctgt ttcgtcaggg tgaggccttt gagcggatgc cgcccgacga actggaacgc
      901 taccgccatc accttgagga gattaaggtc gtcctgatca agcgtctgat gagcgaccaa
      961 ttaccgttca tcggcgtcgc caagcgcgtt ctgaccatcg acgacctgcg tcgcatctac
     1021 agcgggttga tcggcacagg caagatcggc ggcaaagctg ccggcatgta tctggcctgg
     1081 cggatcctgc aagaagtgga cgaacgctac ggccgcgcgg ccatcaatat cccggattcc
     1141 tacttcatcg gcaccgatgt gctctacgag tttttcctga tgaataaact cgagcattac
     1201 atgaatcaga agtatcggtc cgtgcgcgag atacgctcgg agtatccgcg catcgtggaa
     1261 gatttcctgg cttcctcctt gcccgattat ctcgtcgtgc agattcgaga ggtcgtggaa
     1321 tcgttgggtg acagcccgct gatcgtccgc tcatccagct tgctggaaga caacttcggc
     1381 ttttccttcg ctggaaaata caacagctac ttttgtgcca accagggcac gccggaagaa
     1441 aaccttgccc aggtgctcga ggccatccgc cgggtctatg ccagttcggt taaccccgat
     1501 gcactgctgt atcgcaagaa acacgacctg attgattacg acgagcgaat ggccatcctg
     1561 ctgcagcgcg tgaccggtcg ctggcatggc cgctatttct atcccgacat ggccggcgtc
     1621 ggcttcagcg agaacccctt ctgctggaca ccggagattc gccgcggcga aggctttctg
     1681 cgcctcgtgg ccggcatggg cacgcgcgcc gtcgaccgtg tggctcagga ttacccacgc
     1741 ctgatcccgt tgagccaccc cgaactgcgg ccggagacga cggtcgaggg gcaacgcaac
     1801 tttgcccagc gctatatgga ggcggtcgat cgcgagcaaa acggcatcgt gaccgtcccc
     1861 gttcgggaag ccctcctgtc caattcgacg acattgcccc ttgtggcctc gatcgataca
     1921 ggcacttcaa tgctccctat ccagcccgac acaacgatcg gggcggatga tcatatcgtc
     1981 atgactttcg acggtctgac ccaggatgcg actttcatcg aattcatgcg cgaaatattg
     2041 caacatctgg
//
LOCUS       DAOMQG010000513         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21314_length_2047_cov_13.054217, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000513 DAOMQG010000000
VERSION     DAOMQG010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21314_length_2047_cov_13.054217"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="lpdA"
                     /locus_tag="PLC06_16665"
     CDS             complement(<1..1334)
                     /gene="lpdA"
                     /locus_tag="PLC06_16665"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020442043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRO92135.1"
                     /translation="MTDQYDVVVLGAGPGGYVAAIRASQLGLKTAIIEKQYMGGVCLN
                     IGCIPTKALLKNAEVAHTLKHRGKEFGFSFDNLQVDYGVAFKRSRQVSDRLVKGIGFL
                     MKKNKIDVYDGTGKLTGPNTIAVKLNDGKDATLEGKNIIIATGARPRPFPGVEFDDKK
                     IISYVPAILAENAPKSVVIIGGGAIGVEFAYVWANYGVDVTILEMMPHLLPNEEPEVS
                     DVLEKAYKKLGVKFQTGAKVTGIEKTGDGVRVTVEGGEPLEAEVCLVAINFMPNTGDI
                     GLEAAGVKLTERKNVAIDGNMRTNVPHIYAIGDVATDYRLAHVASAMGIVAAETIAGH
                     PTMSLDFRMMPRCTYSQPQVASFGYTEAAAKEAGHEVKVGKFPFQANGKALGLGEGIG
                     FIKIIADARYGEILGAHLVGPDVTELLPELTLARNNELTAEEIARNVHAHPTL"
     gene            complement(1486..1905)
                     /gene="mce"
                     /locus_tag="PLC06_16670"
     CDS             complement(1486..1905)
                     /gene="mce"
                     /locus_tag="PLC06_16670"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRO92136.1"
                     /translation="MIKNINHIAIIVPGLEGALDFWEGALGLKLARTDSVPSEGVDVA
                     FLPVGGSNIELMTPTVADTGVARYLEKRGPGIHHMCLEVDDIEGMMARLREHNIQLIN
                     EQPVEVPSDGAHGSRKYAFVHPKSTGGVLVELYQLNE"
BASE COUNT          321 a          642 c          635 g          449 t
ORIGIN      
        1 agcgtcgggt gggcgtggac gttgcgggcg atctcttcgg ccgtcagctc gttgttgcgg
       61 gccagcgtca gctccggtaa cagttcggtc acgtcggggc cgacgaggtg ggcgcccagg
      121 atttccccat agcgcgcgtc ggcgatgatc ttgatgaaac cgatgccttc gcccagcccc
      181 agcgccttgc cgttggcctg gaaggggaac ttgccgacct tgacctcgtg gccggcctct
      241 ttggctgccg cctcggtgta gccgaagctg gccacctgcg gctggctgta ggtgcagcgc
      301 ggcatcatgc ggaagtcgag cgacatcgtc gggtggccgg cgatcgtctc ggccgccacg
      361 atccccatgg ccgaggcgac atgggccagc cggtagtcgg tggccacatc gccgatggca
      421 tagatatggg gcacgttggt gcgcatgttg ccgtcgatgg cgacgttctt gcgctcggtc
      481 agcttcacgc cggccgcttc cagaccgatg tccccggtgt tgggcatgaa gttgatggcc
      541 accaggcaga cctcggcctc cagcggctcg ccgccctcga cagtgacgcg cacgccgtcg
      601 ccggtcttct cgatgccggt gaccttggcc cccgtctgga acttgacgcc cagcttcttg
      661 taggccttct ccagcacatc gctgacttcc ggttcctcgt tgggcagcag gtggggcatc
      721 atctccagga tggtcacatc cacgccgtag ttggcccaca cgtaggcgaa ctcgacgccg
      781 atggccccgc cgccgatgat gacgaccgac ttgggcgcgt tttcggccag gatggccggc
      841 acgtaggaga tgatcttctt gtcgtcgaac tccacgccgg ggaacggccg cggccgcgcg
      901 ccggtggcga tgatgatgtt cttgccctcc agcgtcgcgt ccttgccgtc gttgagcttg
      961 acggctatcg tgttcggccc ggtcagcttg cccgtgccgt catagacgtc gatcttgttc
     1021 ttcttcatca ggaagccgat gcctttcacc agccggtcgg atacctggcg gctgcgtttg
     1081 aaggccacgc cgtagtcgac ttgcaggtta tcgaagctga agccgaactc cttgccgcga
     1141 tgtttcagcg tgtgggctac ctcggcgttc ttgaggagcg ccttggtggg gatgcagccg
     1201 atgttgaggc agacgccgcc catgtattgc ttctcgatga tggccgtctt cagccccagc
     1261 tgcgacgcgc gaatggcggc gacatagccg cccggcccgg ccccaagaac tacgacatcg
     1321 tattgatcgg tcattttgat ctccagtgtt cagtggtcag tagatcaatg gtcagtagat
     1381 tagtggtcag tgatcagtgg ccagtaagcc gtggagcgtc tctccggcgc cccttgctga
     1441 cctactgacc tactgatcta ctggcctact gaatactgat ctactctatt cattcaattg
     1501 atataactcc accagcacgc cccccgtgct cttcgggtgg acgaaggcgt atttgcggct
     1561 gccgtgggcg ccgtcggacg gcacctccac cggctgctca ttgatgagct ggatgttgtg
     1621 ctcgcgcagg cgggccatca tcccttcgat gtcgtcgacc tccaggcaca tgtgatggat
     1681 gcccgggccg cgcttctcca gatagcgggc cacgccggtg tcggcgacgg tcggcgtcat
     1741 cagctcgata ttgctgccgc cgaccggcag gaaggccacg tcgacgccct cgctgggcac
     1801 cgaatcggtc cgcgccagct tcaggcccag cgcgccttcc cagaaatcca gcgccccctc
     1861 cagtccgggg acgataatcg cgatgtggtt gatgtttttg atcataatat tgcctgtagc
     1921 acagacattc ctgtctgtgt agaggctcag tcaggaatgt ctgagatact acctgagcta
     1981 ccgcagcaaa ttggcgatga ccgccttttc ctcgcgcagt tcgtcctggg tgatggcgat
     2041 cttctct
//
LOCUS       DAOMQG010000514         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21315_length_2047_cov_10.596888, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000514 DAOMQG010000000
VERSION     DAOMQG010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21315_length_2047_cov_10.596888"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..729)
                     /locus_tag="PLC06_16675"
     CDS             complement(40..729)
                     /locus_tag="PLC06_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020058300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HRO92137.1"
                     /translation="MSDEINLKEMLDEDIYELMNEDLYDGYAQEVVEEVYEMLARGHA
                     PYDILSKGLIAGMDIVGDDFRDGILFVPEVLMAAKAMKAGMAILRPLLAETGAEKIGT
                     MVVGTVKGDIHDIGKNLTAMMMEGAGFEVYDLGVNNSVDNFMTAIEMRKPDIIGMSAL
                     LTTTMPYMRVVIDALVEAGIRQDLIVLVGGAPLNDAFAKDIGADAYCRDAAIGVETAK
                     RLMQQRREKSR"
     gene            complement(785..>2047)
                     /locus_tag="PLC06_16680"
     CDS             complement(785..>2047)
                     /locus_tag="PLC06_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRO92138.1"
                     /translation="GLAKMQKSIIDTLNTLATRAARQSGLTARDIHEAVIVGNTTMIH
                     ILLGINPQELGSAPFALAIREAIDLKARELDVRLHPGAYVHVLPAEAGHVGADNVAVL
                     LAEEPYRQEKTTLIVDVGTNAEIVLGNRERLYSASSPTGPAFEGAQITFGMRAAPGAI
                     ERVRIDPMTWEARFRVIGEERWSDQWPTANGEEPSQHLAAGICGSGIIEVVAELFMAG
                     IISGDGRFNASRPHPRLVQQGKTTAYILAYEAETAGGQPILIAQDDIRSVQLAKAALY
                     SGIKLLMKRSSIERVERIILAGAFGSYIDPKHAMILGLIPDCNLEQVVAVGNAAGDGA
                     RIALLNRYKRVEAQQQARDVTYIETAADPDFQDEFVSALHIPHISDGFPFLEKALGKA
                     LPGRDENNEENKRMRRAERLRDRQRQKS"
BASE COUNT          385 a          628 c          544 g          490 t
ORIGIN      
        1 agttgggcca atttgctgat catactgctc ctttgttgcc tagcgactct tctctcgccg
       61 ttgttgcatg agccttttgg cagtctctac tccgatcgct gcatcccggc aataggcatc
      121 ggcgccgata tccttcgcaa aagcgtcgtt gagcggtgcg ccgccgacca ggacgatcag
      181 gtcctgcctg atgcccgctt ccaccagcgc gtcgattacc acgcgcatat aaggcattgt
      241 ggtcgttagc agggcactca tgccgatgat gtcaggcttg cgcatttcga tggcggtcat
      301 gaagttgtcg acggaattat tcacgccaag atcgtacacc tcgaacccgg ccccttccat
      361 catcatggcc gtcaggtttt taccgatgtc gtggatatct cccttgacgg tccccaccac
      421 catcgtgccg atcttctccg cgccggtctc cgccagaaga ggccggagga tggccattcc
      481 cgccttcatg gccttggccg ccatgaggac ctcgggcaca aacaggatcc catcgcgaaa
      541 gtcatcgccg acaatatcca tgcccgcgat caatcccttg ctcagaatat cgtaaggagc
      601 atgcccgcgt gccagcatct cgtagacttc ttcgacgacc tcctgggcat agccgtcgta
      661 taggtcttcg ttcatcaact catagatatc ttcgtcgagc atctctttca aatttatctc
      721 gtcgctcatt gtgaacctct ggttggagat atgtgccatc gactggtgtc ttgtccgggc
      781 ctttttacga tttctgcctt tgtcgatcgc gtagccgttc ggctcggcgc atgcgcttgt
      841 tttcctcatt gttttcatcg cgccccggca atgccttgcc cagcgccttt tccagaaacg
      901 gaaaaccatc ggagatatgc gggatgtgga gcgcgctgac gaactcatcc tgaaaatcag
      961 gatcggcggc cgtttcaatg taagtcacat cgcgagcttg ttgctgagcc tccacgcgct
     1021 tgtagcggtt cagcagggcg atgcgcgcgc cgtccccggc cgcgttccct acggcgacaa
     1081 cctgttccag attgcaatcc ggtatcagtc ccaagatcat agcatgtttt ggatcgatgt
     1141 agctgccgaa tgcaccggcc aggataattc gctccacgcg ctcaatcgac gaccgcttca
     1201 tcaataactt gatcccggaa tacagggccg ctttcgccaa ttgtacactg cgtatgtcat
     1261 cctgggcgat gaggatgggt tgtccgccgg cggtctccgc ttcataggcg aggatgtacg
     1321 ctgtcgtttt cccttgttga acaagtcgcg ggtggggacg tgaagcatta aagcggccgt
     1381 cgccgctgat gatcccggcc atgaacaatt cggctacaac ctcgatgatc cccgagccgc
     1441 aaataccggc ggcgagatgt tgcgatggtt cctctccatt cgctgtcggc cattgatccg
     1501 accaccgttc ctcgccgatt acgcggaatc tggcctccca cgtcattgga tcgatgcgca
     1561 cgcgctcgat cgcccccggc gcggctcgca tgccgaacgt gatctgtgcc ccttcaaaag
     1621 ccgggccggt cgggctggaa gcgctataga ggcgttcccg attgcccagg acgatctccg
     1681 cgttggtgcc aacgtcaacg atgagcgtgg ttttttcttg acgataaggt tcctcggcga
     1741 gcaatacggc tacattatcc gcgccgacat gccccgcctc tgccggcaag acatggacgt
     1801 aggctcccgg gtggagccgc acatcaagct ctcgcgcttt caggtctatt gcctcgcgaa
     1861 tagctaatgc aaacggcgcg ctccccagtt cctggggatt aatacccaac agaatatgaa
     1921 tcatcgtggt gttgccgacg attaccgcct cgtgaatgtc gcgcgccgtc agaccggact
     1981 ggcgtgcagc gcgtgtagcc aacgtattca gcgtgtcgat gatcgacttt tgcatcttcg
     2041 ccaggcc
//
LOCUS       DAOMQG010000515         2038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21432_length_2038_cov_6.303076, whole genome shotgun sequence.
ACCESSION   DAOMQG010000515 DAOMQG010000000
VERSION     DAOMQG010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21432_length_2038_cov_6.303076"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLC06_16685"
     CDS             complement(<1..445)
                     /locus_tag="PLC06_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92139.1"
                     /translation="MSNTELRSYVFLDNLQPQHAAFLGTVAGGFLPLPGDASLWIEIS
                     PGIEINRITDVALKSTDVRPGMQVIERLYGLLEVHSSSQASVRAAGAAILAELGVSES
                     DRIKPKVISSQIIRNLDPHQTQLINRVRHGQMIMAGQTLYVMEVAP"
     gene            complement(448..723)
                     /locus_tag="PLC06_16690"
     CDS             complement(448..723)
                     /locus_tag="PLC06_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HRO92140.1"
                     /translation="MSTIRCEECGIGRCHPVTTPYLLQIGRHMLVLPDSPAYTCDICG
                     NRFFDDEFLTGVHYLLEQAAEDSRRRARRRQAPRPEPAALPQARRSR"
     gene            complement(727..1383)
                     /gene="deoC"
                     /locus_tag="PLC06_16695"
     CDS             complement(727..1383)
                     /gene="deoC"
                     /locus_tag="PLC06_16695"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRO92141.1"
                     /translation="MNIAKYIDHTLLKADATQDQIAQLCYEARTHHFASVCVNPANVR
                     LCAQLLKGSDVKVCTVVGFPLGATAAPVKAYEAQTAIRDGATEIDMVINVGALKSHDY
                     RAVSEDIAGVVATTHAHNALVKVILETALLTDEEKVIASQLSKAAGADFVKTSTGFGP
                     GGATAEDVALMRRVVGPNLGVKASGGVRNYADTQAMIAAGATRIGASAGVAIVSEARR
                     "
     gene            complement(1635..>2038)
                     /locus_tag="PLC06_16700"
     CDS             complement(1635..>2038)
                     /locus_tag="PLC06_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRO92142.1"
                     /translation="DPVDYPDIAVAVARRVASGDAWRGIIIDGAGIGSCMAANKISGV
                     RAAMCYDQATAVNSREHNNANVLTLGAGLIGPNLAQLIVKTWLATEFGGGRHAARVAK
                     IDALDGARPTVAQTAAPTGGAVKSTTPPPAY"
BASE COUNT          348 a          643 c          680 g          367 t
ORIGIN      
        1 ccggggccac ttccatcaca tagagcgtct gcccggccat gatcatctgc ccatggcgaa
       61 cgcgattgat gagctgcgtc tgatgggggt ccagattgcg aatgatctgg ctggagatga
      121 ccttcggctt gatgcgatcc gactcggaca cccccagttc agcgaggatg gccgcgccgg
      181 cggcgcgcac gctggcctgg ctggatgaat gcacctcaag cagcccatag agccgctcga
      241 tgacctgcat ccccggccgc acgtcggtgg atttcagcgc cacgtcggtg atgcgattga
      301 tctcaatgcc gggcgaaatc tcaatccaca gcgaggcgtc gccgggcagc ggcaagaagc
      361 cgccggccac cgtgccgaga aaggcggcgt gctggggctg gagattgtcg aggaaaacgt
      421 aactgcgcag ttcggtattg gacacgatta gcgactccta cgggcctggg gcagcgcggc
      481 cggttcgggg cgcggggcct gtcgccgccg cgcgcgtcgc cgggaatcct cggccgcctg
      541 ttccaaaagg taatgaactc cggtgaggaa ttcgtcatca aagaaacgat tgccgcaaat
      601 atcgcaggtg taggccggcg aatcgggcag gacgagcatg tgccttccta tctggagcag
      661 atagggcgtc gtcaccgggt gacagcggcc gattccacat tcttcgcaac ggatcgttga
      721 catgtgttat ctcctggctt cggaaacgat ggccacgccg gcgctggcgc cgattcgcgt
      781 ggctccggcg gcgatcatcg cctgggtgtc ggcatagttg cgcaccccgc cggacgcttt
      841 gacgcccaaa ttcgggccga cgacgcggcg catgagcgcg acgtcctcgg ccgtggctcc
      901 gcccggccca aagccggtcg aagtcttcac gaaatccgcg cccgccgcct ttgaaagctg
      961 actggcgatg accttctctt cgtcggttag cagggccgtc tcgaggatca ccttcaccag
     1021 cgcgttgtgg gcgtgggtcg tggccacaac cccggcgatg tcctcgctga cggcgcgata
     1081 gtcatgggac ttgagcgcgc cgacgttgat gaccatgtcg atctccgtcg cgccatcgcg
     1141 gatggcggtc tgggcctcgt aggctttcac cggcgcggcc gtggccccca gcgggaagcc
     1201 gacgacggtg caaaccttca cgtcggagcc tttcagcagt tgggcgcaca gccgcacgtt
     1261 ggccggattg acacacaccg aagcgaaatg gtgagtgcgc gcctcataac aaagctgggc
     1321 gatctgatcc tgtgtggcgt cggccttcag cagcgtgtgg tcaatgtatt tggcgatgtt
     1381 gatatcctgg ggcagcgcac ccagccgcga ggtgacacgc gaggcgccgg ccgatacgac
     1441 acggccgacc ttgtcggcgc aatgggccac gcagctgccg tcgctgcagt tgcagccgtc
     1501 gggccggggc gcggccggag cgccggagcc aaggcgcgac aggacttcgc gggtcacttc
     1561 ttcgatgaca cgttcaacgg tagcagaatc gtaggtggtc attcagtcgc catttactca
     1621 gtcgaaccgc acacctaata ggcgggcggg ggagtcgtcg attttaccgc gccgccggtc
     1681 ggggcagcag tttgggccac ggtcggccgc gcgccgtcga gcgcatcgat cttggccacg
     1741 cgagcggcgt gccgtccgcc gccaaattca gtcgccagcc acgtcttcac gattagctgg
     1801 gccaggttgg ggccgatgag gcccgcgccc agcgtcagaa cgttggcgtt gttgtgttcg
     1861 cggctgttca cggccgttgc ctgatcatag cacattgccg cccgcacacc actgatcttg
     1921 ttggcggcca tgcagctgcc gatgcccgcg ccgtcgatga tgatgccgcg ccaggcgtcg
     1981 cccgaagcca cgcgccgggc cacggccacg gcgatgtcgg gatagtcaac cgggtcct
//
LOCUS       DAOMQG010000516         2029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21536_length_2029_cov_8.740122, whole genome shotgun sequence.
ACCESSION   DAOMQG010000516 DAOMQG010000000
VERSION     DAOMQG010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21536_length_2029_cov_8.740122"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1719
                     /locus_tag="PLC06_16705"
     CDS             334..1719
                     /locus_tag="PLC06_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HRO92143.1"
                     /translation="MKFVTRIRRPAGRLLVLLAALAIIVALWPDATSLYDLTGEEQPR
                     GQIAGIAHWLNTAIRPQPDLQPAAVAGSPYTFPAVSPFGVNTFLQQEAEETKRATSLD
                     LAHAAGLRYIRQEFTWEDIEIHGKGDFIDRRNDPAGVDAWLKYDHIVDLAEARDLQII
                     ARLSNPPAWSRAAGDEMGEKAPPDDFNDFGDFVAAVAGRYRGRIRTYQVWNEPNGNEE
                     WGLQDVDPEAFTQLLCLAYERIKAADPDAVVLAGALTPTVANDGRHMNDLIYLERMYA
                     AGAGGCFDVMSAQGYGLWSGPTDQRLRPTVINYPHNLFLRDIMVRHGDAAKPIWISEM
                     GWNVAPEHVRPDFGRVTEEQQARYAVGAYRRAQEEWPWVGVNAYWFLKRPADWEIDQA
                     WYYFRMLEPDFTPLPVYDAVADYATAVPDLSPMPGWKYDWMRARPGLFTFGAAVLFFS
                     LLRALAPRDGR"
     gene            1716..>2029
                     /locus_tag="PLC06_16710"
     CDS             1716..>2029
                     /locus_tag="PLC06_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92144.1"
                     /translation="MSDTRRLLPAAVFVLLLATFTRFHLLEAQSFWNDEGNSARLSER
                     SIPAILEGTASDIHPPLYYLALRGWRELLGETEFALRSLSAFAGVLVVAAVMALAAKG
                     GRG"
BASE COUNT          353 a          636 c          636 g          404 t
ORIGIN      
        1 cacgaacagg gttttttcga agggagacag catgttctct tcctctcctt tcgccaccga
       61 cagggaaacg gggtcggggc gatgcttgtc aatgagccgg atcgttccgt ggcggaagtt
      121 taactcaccc gacgattcgg gcaagaagtc tgcgcatgtg gcgatgccgg cgactcatac
      181 cgctctatta taacgccaat ttgacgcact gcatcataac aaatgtcatg tgtttggggt
      241 aaatacggga attcagcgaa tgtccgacgc tgctgcgccg ttctccctgc gcaaactagg
      301 tccaatgccg ccacatcgct ataatcggcg gtcatgaaat tcgtcactcg cattcggcga
      361 ccggccgggc gattacttgt cctgctggct gccctggcga tcatcgtcgc cctctggccc
      421 gacgccacgt cgctttatga cctgaccggc gaggagcagc cacgcggcca gatcgccggc
      481 attgcccatt ggctcaacac cgccattcgg ccgcaacctg atttgcaacc ggcggccgtt
      541 gccggatcgc cctatacctt cccggccgtg tcccctttcg gtgtcaacac tttcctgcag
      601 caggaggccg aggaaaccaa gcgcgccacc agtctcgatc tggcccacgc cgccgggctg
      661 cgctacatcc gccaggagtt tacctgggaa gatattgaga tccacggcaa aggagatttc
      721 atcgaccggc gcaacgatcc ggccggggtg gatgcctggc tcaagtacga ccacatcgtc
      781 gatctggccg aggcgcgcga tctccagatc atcgctcgcc tcagcaaccc gcccgcctgg
      841 agccgcgccg ccggcgacga aatgggcgag aaggccccac ctgacgactt caacgacttt
      901 ggcgattttg tcgctgccgt tgccgggcgc taccgtggcc gtatccgcac ctatcaggtg
      961 tggaacgagc cgaacggcaa cgaggaatgg gggttgcagg acgtcgatcc cgaagccttc
     1021 acccaattat tgtgccttgc ctatgagcgt atcaaggcgg ccgatcccga tgccgtggtg
     1081 ctggccggag cgctgacgcc gaccgtggcc aacgacggcc gccacatgaa cgacctcatc
     1141 tatctggagc gcatgtatgc cgccggggcg gggggatgct tcgatgtcat gtcggcccag
     1201 gggtatggct tgtggtccgg ccccaccgac cagcgattgc ggccgacagt catcaactat
     1261 ccccacaatc tcttcctgcg cgacatcatg gtgcgccacg gcgatgccgc caagccgatc
     1321 tggatcagcg agatgggctg gaacgtagcc ccggagcatg tgcggcctga ctttggtcgc
     1381 gtcacggaag agcagcaggc gcgctatgcc gtgggcgcat accgtcgtgc ccaggaagaa
     1441 tggccgtggg ttggtgtgaa cgcctactgg tttctgaagc gcccggccga ttgggagatc
     1501 gaccaggcgt ggtactattt tcgcatgctg gaaccggatt tcacgccgct gccggtctat
     1561 gatgctgtgg ccgattatgc caccgctgtg ccggacctgt cgcccatgcc tggttggaaa
     1621 tacgactgga tgcgcgcccg gccggggctg ttcacctttg gcgcggcggt gttgtttttc
     1681 agcctgttgc gcgcccttgc cccgcgagac ggccgatgag cgacacccga cgattgctgc
     1741 ccgcggccgt gttcgtgctg ctgctggcca ccttcacccg cttccacctg ctggaggcgc
     1801 aatccttctg gaacgacgaa ggtaacagtg ctcgcctcag cgagcgttct atcccggcta
     1861 ttctggaggg cacggcgagc gacatccacc caccgctcta ctacctggct ctgcgcggct
     1921 ggcgcgagct gctgggcgag acggagttcg ctctgcgctc gctgtcggcg ttcgcggggg
     1981 tgttggtggt ggcggcggtg atggcattgg cggcgaaggg gggaagggg
//
LOCUS       DAOMQG010000517         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21577_length_2026_cov_6.372907, whole genome shotgun sequence.
ACCESSION   DAOMQG010000517 DAOMQG010000000
VERSION     DAOMQG010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21577_length_2026_cov_6.372907"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2026
                     /locus_tag="PLC06_16715"
     CDS             <1..>2026
                     /locus_tag="PLC06_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRO92145.1"
                     /translation="ADYIMISSQRAIWNLPRMPMTYPLMMKYYESLFNGALGFELVAQ
                     FHEDTRLGPLYISDTTAQIGWGEPPQIGWPPPGELAAEEAFSVYDHPPVWIFRKTDAY
                     DPAATRALLERVDLSNVVVQNPLEATQARNGLMMSDYERGLQEAGGTFSEVFAIDGFL
                     SRNPWAAAAAWMAALVLLGWAAFPLAFVALRGLPDRGYALSRVLGLLVIAYLPWLAAS
                     IKFLPFSRGTILLALAALLGLSATIAWRQRDALRAFIRERSGYIVLVELLGVALFLIM
                     IAIRLGNPDVWDVIWGGEKPMDLSYFTAVMRSTVFPPYDPWHAGGYINYYYFGFVLVG
                     TLAKLLRVVPAVAYNLALPMLFSMTGLGVFSIAWGLVERVETEFLERNSVSKGFMQVI
                     RSRAAGAGFIAVALALLLGNLAQVTVIGSAWHNSGSPSLGELPIIGGAARTLDGGLKV
                     LSGQPVAMYPGDWFWLASRAINANPGEVQPITEFPFFTFLYADLHAHMIAMPLQMLAL
                     GWAVAVVLAARNTKDEIRNTSRRRSELVSRISYLVSFFVGALVIGSLRPTNTWDWPTY
                     LLLGSLAVAYAVIRAGGWSWRAAGRAGFAALALVGLSSLLFLPFSANYGSAYSSASLW
                     PGSWTHAWNYLLVYGLFLFFIVTHLIRELRDWTASWTEEGKRRMEPYGWVV"
BASE COUNT          291 a          678 c          663 g          394 t
ORIGIN      
        1 gccgactaca tcatgatcag cagccagcgg gccatctgga acctgccgcg catgcccatg
       61 acctacccgc tgatgatgaa atattacgag tcgctgttca acggcgcgct cggcttcgag
      121 ctggtggccc agttccacga ggacacccgc ctcggcccgc tctacatcag cgacaccacc
      181 gcccaaatcg gctggggtga gccgccgcaa atcggctggc cgccgccggg cgagctggcc
      241 gccgaggaag cgttcagcgt ctacgaccac ccgccggtgt ggattttccg caagaccgac
      301 gcctatgatc cggcggccac gcgagcgctg ctggagcgcg tcgacctgag caatgtcgtt
      361 gtccagaacc cgctggaagc gacacaggcc cgcaacggtc tcatgatgag cgactacgag
      421 cgcggattgc aggaagcggg cggcaccttc agcgaggtgt tcgccatcga cggcttcctc
      481 tcgcgcaacc cgtgggcggc ggccgcggcc tggatggcgg cgctggtgct gctgggttgg
      541 gcggcgttcc cgttggcgtt cgtggccctg cgcggcctgc ccgaccgcgg ctatgccctg
      601 tcgcgggtgc tggggctgct ggtcatcgcc tatctgccgt ggctggcggc gagcatcaag
      661 ttcctgccct tctcgcgcgg gacgatcctg ctggctctgg cggcgctatt gggcctcagc
      721 gcgaccatcg cctggcgaca acgcgacgcg ctgcgggcat tcatccgcga gcgctccggc
      781 tacatcgtgc tggtcgagct gctgggcgtg gccctgttcc tgatcatgat cgccatccgg
      841 ctgggcaacc ccgacgtgtg ggacgtcatc tggggcggcg agaagccgat ggatctgtcc
      901 tatttcaccg ccgtgatgcg ctccaccgtc ttcccgccct atgacccgtg gcacgccggc
      961 ggctatatca actactacta cttcggcttc gtcctcgtgg gcacactggc caagctgctg
     1021 cgggtcgtgc ccgcggtggc ctataacctg gccttgccga tgctgttcag catgaccggg
     1081 ctgggggtgt tcagcatcgc ctggggattg gtggaacggg tagaaaccga gtttctcgaa
     1141 agaaactcgg tttctaaggg cttcatgcaa gtcattcgtt cccgcgccgc cggcgccggg
     1201 ttcatcgccg tggccctggc gctgctgctg ggcaatctgg cgcaggtgac ggtcatcggc
     1261 agcgcctggc acaactccgg ctccccttcg ctgggagagc tgccgatcat cggcggcgcg
     1321 gcgcgcacac tcgacggcgg cctgaaggtg ctgagcggcc aaccggtggc catgtacccc
     1381 ggcgactggt tctggctggc ctcgcgggcc atcaacgcca accccggcga ggtgcagccg
     1441 atcaccgaat tcccgttctt caccttcctc tacgccgacc tgcacgcgca catgatcgcc
     1501 atgccgctgc agatgcttgc cctgggctgg gcggtggcgg tggtgctggc cgcccgaaat
     1561 acgaaagacg agatacgaaa tacgagtaga agacgctctg aactcgtatc tcgtatctcg
     1621 tatctcgtat ctttcttcgt tggcgctctc gtcatcggct ccctgcggcc gacgaacacc
     1681 tgggactggc cgacgtatct gctgctgggg tcgctggcgg tggcctatgc cgtcattcgc
     1741 gccggcggct ggagttggcg ggcggcgggt cgggcaggct tcgccgcgct ggcgctggtg
     1801 gggctgtcgt cgctgctgtt cctgcccttc agcgccaact acggctcggc ctactcttcg
     1861 gcctcgctgt ggcccggctc ctggacccac gcctggaact acctgctcgt ctacggcctg
     1921 ttcctgttct tcatcgtcac ccacctcatc cgcgagttgc gcgactggac ggcctcgtgg
     1981 accgaggaag gcaagcggcg catggagcca tacggctggg tggtcg
//
LOCUS       DAOMQG010000518         2021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21632_length_2021_cov_7.485249, whole genome shotgun sequence.
ACCESSION   DAOMQG010000518 DAOMQG010000000
VERSION     DAOMQG010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21632_length_2021_cov_7.485249"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLC06_16720"
     CDS             <1..250
                     /locus_tag="PLC06_16720"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRO92146.1"
                     /translation="VGQEIIARMESRGKLAKRLVRLRPGAPVEQGAEISAGGRAVGTI
                     TSAADGPAGPLALGYVKTTALTEVTALVVGEIPLSIIN"
     gene            310..1863
                     /locus_tag="PLC06_16725"
     CDS             310..1863
                     /locus_tag="PLC06_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRO92147.1"
                     /translation="MAEFFENNLVVIYFFYGLAFFSMGLAIWLTSSRFRTSEFRLAGA
                     LLFLAGFGIVHGLQEWFDMFRLLDERGSTIIPYWLLLHEVHLIHLVISFLLLVFFGIK
                     LLFANRRTTINGNHMALLGAGAFLALWMLSVGLTWLVYRPERLVMINAADVLARYTLG
                     MTGSVIAAWAIWLEQRNFKAHGMPQFGRLLLGAAVALLLYGVIGQLFVQPSFFFPSTV
                     INSETFLDVFGFPIQLLRAVLALLMAYFIIRALTAFEIENQRRLADAVEQRAAAQQEA
                     LRVQQQARIETEQLNRQLQSLLHRIVSAQEAERKRIARELHDGTGQTLTALGLGLAAA
                     ADRIDSDPAAVRRQLTDMKQMNAQVLQEVHNVISDLRPSILDNLGLVPALRGHISAFE
                     SRTGIQAQLVVHGKTARLKPEVETTVFRIVQESLTNVMRHAAASSVLVEVTFNETGVE
                     LAIRDDGRGFDVARALTGEGGRAAWGLLGIHERASLVGGSAELLSSPGEGTTVRVSIP
                     QPFNEVDHE"
     gene            1856..>2021
                     /locus_tag="PLC06_16730"
     CDS             1856..>2021
                     /locus_tag="PLC06_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010355641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRO92148.1"
                     /translation="MSETIRLLLADDHAVVRSGLRLLLEAQPDLAIIGEAENGEEAIR
                     RTAELRPDVVL"
BASE COUNT          338 a          624 c          656 g          403 t
ORIGIN      
        1 cgtgggacag gagatcatcg cccggatgga gagccgcggc aagctggcca agcggctggt
       61 gcggctgcgg ccgggcgcgc cggttgagca gggcgcggag ataagcgcgg gcggccgcgc
      121 tgtggggacg atcacctcgg ctgccgacgg ccccgccggg ccgctggcgc tgggctatgt
      181 caagacgacg gctctaaccg aggtgacggc gctggtggtg ggagagattc ctttaagtat
      241 catcaattga acaactgtag aaaccgggtt tctcggaaga aacccggttt ccaggttatg
      301 gcaaaaccga tggccgagtt tttcgagaac aacctcgtcg tcatctactt cttctatggc
      361 ctggccttct ttagcatggg gctggccata tggttgacgt cgtcgcgttt ccgcacgtcg
      421 gagttccggc tggccggggc gctgctcttt ctcgccggct tcggcatcgt ccacggcctg
      481 caggagtggt tcgacatgtt ccgcctgctc gacgagcgtg gcagcacgat catcccctac
      541 tggctgttgc tccatgaagt tcacctgatc catctggtca tctctttcct gctcctggtc
      601 ttttttggca tcaagcttct gtttgccaat cgccggacga ccattaatgg caaccatatg
      661 gccttattgg gggcaggcgc gttcctcgcg ctgtggatgc tcagcgtcgg gctgacatgg
      721 ctcgtgtatc gtccggagcg gctggtcatg atcaacgcgg ccgatgtgct ggcccgctac
      781 acgctcggta tgaccggctc ggtcatcgcc gcctgggcga tctggctgga gcagcgcaac
      841 ttcaaggccc acggcatgcc ccagttcggc cgtctgctat tgggcgcggc ggtggccttg
      901 ctgctctatg gcgtcatcgg tcaattgttc gtccagccca gctttttctt cccgtcaacg
      961 gtcatcaatt ccgaaacgtt cctcgatgtg ttcggctttc cgatccagct cttgcgcgcg
     1021 gtgctggcct tgctgatggc ttacttcatc attcgcgctc tgactgcttt tgagatcgaa
     1081 aaccagcgac gattggccga tgccgtggag cagcgcgccg ccgcgcaaca ggaagcgttg
     1141 cgggtgcaac agcaggcgcg aattgagacc gagcagctca accggcagct acaatcgctg
     1201 ctgcaccgga tcgtgtcggc ccaggaagcg gagcgcaaac gcatcgcccg cgaactccac
     1261 gacggcacag gccagacgct gaccgctctg gggctgggac tggccgccgc ggccgaccgc
     1321 atcgacagcg atccggcggc cgtccgtcgc caattgacgg acatgaagca gatgaacgcc
     1381 caggtgttgc aggaagttca caacgtcatc tccgatctgc ggccgtcgat tctcgacaac
     1441 ctgggcctcg tgcccgcctt gcgtggccat atcagcgcgt tcgaatcgcg gacgggcata
     1501 caggcgcaac tcgtcgtcca cggcaaaacc gcgcgcctga agccggaagt ggagacgacg
     1561 gtttttcgta tcgtgcagga gtcgctgacc aatgtgatgc gccacgccgc cgcgagcagc
     1621 gtgctggtgg aggtcacgtt caacgaaacc ggagtcgagc ttgctatccg ggacgacggc
     1681 cggggcttcg acgtagcccg cgcgctgacc ggagaaggcg gccgcgccgc ctggggcctg
     1741 ctcggcatcc atgagcgcgc gtctctcgtc ggtggttcgg ccgagctgct gtccagcccc
     1801 ggcgagggaa ccaccgtgcg cgtgtccatt ccgcagccat ttaatgaggt tgaccatgag
     1861 tgaaacgatc cgcctgttgt tggccgacga ccatgccgta gtccgttccg ggctgcggct
     1921 gctgctggaa gcgcaaccgg acctggcgat catcggcgag gccgagaacg gcgaggaggc
     1981 catccggcgc acggccgagt tgcggcccga cgtggtgctg a
//
LOCUS       DAOMQG010000519         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21652_length_2020_cov_7.922646, whole genome shotgun sequence.
ACCESSION   DAOMQG010000519 DAOMQG010000000
VERSION     DAOMQG010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21652_length_2020_cov_7.922646"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLC06_16735"
     CDS             <1..495
                     /locus_tag="PLC06_16735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HRO92149.1"
                     /translation="VVDVGTGSGCIAISLARYLPRAEIAAIDVSPQALEIAAANVTRH
                     VPGQVGLVRADLLSSIAPGLDLIAANLPYIAGHEWPTLSIGVKSYEPTLALHGGEDGL
                     DLIGALLPQAAERLRPGGLVLLEIGWQQGEAVAMLAQKWFPTARMAVRADFAGHDRIV
                     TIET"
     gene            542..1186
                     /locus_tag="PLC06_16740"
     CDS             542..1186
                     /locus_tag="PLC06_16740"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRO92150.1"
                     /translation="MNIGTLKTHYLRTGRPAAIIEAAACLHRGLPVVFPTDTVYGVGV
                     MPFDEAAIERLYALKGRPADKGIPILLSDPIHLERVARMVPPVAQSLIERFWPGPLTL
                     IVPRAPGLPELISPNDTIAVRIPDHEVARALIREAGGAVATSSANLSGQEPAHTGEAA
                     MAALQGLVAAVLDDGPTPGSRPSTIVDCTSMQPVVLREGPLSAADLGLELYSAG"
     gene            1193..1786
                     /locus_tag="PLC06_16745"
     CDS             1193..1786
                     /locus_tag="PLC06_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRO92151.1"
                     /translation="MSGILLPLLLFAGAIVATFLTYRNIRRGGARFYTLEREIVLRRA
                     MVTLVAASVLYTAALGLIYMQRQQLIEALQPPEEQEAADGTTLPAITTPTPMLEVFPP
                     TPEPTSLTPAPTATVTPTVCRAVVEGTGGNGIYLRDAPQGEQLVILPDGTLLTVLEDA
                     PVEAGGFVWRKVRAVGGEEGWAAQDFLTIRAPCGSTD"
     gene            1807..>2020
                     /locus_tag="PLC06_16750"
     CDS             1807..>2020
                     /locus_tag="PLC06_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRO92152.1"
                     /translation="MIIGIDASRTTTSHRTGTEAYAYRLIDALLPPAADRGHRLRLYF
                     NQSPAADLFPASEHVERVIIPQRRLWT"
BASE COUNT          345 a          665 c          622 g          388 t
ORIGIN      
        1 gtcgtcgacg tgggcacagg cagcggctgc atcgcgatca gcctggcgcg ctacctgccg
       61 cgagccgaga ttgcggcaat tgatgtctcg ccgcaagcgc tggaaattgc ggccgccaat
      121 gtgacccgcc acgttccggg acaagtgggg cttgtgcggg ccgatctgct gtcttccatc
      181 gcccctgggc ttgatctcat tgccgccaac ttgccttaca tcgccgggca tgagtggccg
      241 actttgtcta ttggagtaaa atcctacgag cctacactcg ctttacatgg tggagaggat
      301 ggcctcgacc ttataggggc gcttttgccg caggcggccg aacgtttgcg tcccggcgga
      361 ctggtgcttt tggagatcgg ctggcagcag ggtgaagccg tcgccatgtt agcccaaaaa
      421 tggtttccta cggctcgcat ggcggtgcgg gccgattttg ccggccacga tcggatcgtg
      481 accatcgaga cctgaataaa ccaggaagac cacagttact agaccacagt tattaatgga
      541 attgaacatc ggaactctga agacccatta tttgcgaacg ggcaggcccg cggcaatcat
      601 cgaagcggcc gcctgcctac atcgcggcct tccggtcgta ttcccgaccg acacggttta
      661 tggcgtcggc gtcatgcctt ttgatgaggc cgccattgaa cgactttatg ccctgaaggg
      721 ccggccggcc gacaagggca tcccgatcct gctctccgac ccgattcatc tggaacgggt
      781 ggccaggatg gttcctcccg tcgcccaatc cctcattgag cgcttttggc cggggccgtt
      841 gacgctcatt gtgccgaggg caccgggctt gcccgaactc atctctccca acgataccat
      901 cgccgtgcgc atccccgacc atgaagtcgc ccgtgccctc attcgcgagg cgggcggcgc
      961 ggtggctacc agcagcgcca atctgagcgg ccaggagccg gcgcacaccg gagaggcagc
     1021 gatggccgcg ctgcaaggtc tggtggcggc cgttctcgat gacgggccga cacccggatc
     1081 tcgcccgtct actatcgttg attgcacgag catgcaaccc gtcgtcctgc gcgaggggcc
     1141 gctttcggcc gccgatttgg ggctggagct ttattcggcc ggctagttga agatgtccgg
     1201 gatactcttg cctcttctcc tttttgccgg cgcgattgtg gcgaccttcc tcacctatcg
     1261 caacattcgg cgcggcggcg cgcggttcta cacgctggag cgcgagatcg tcctgcgtcg
     1321 cgccatggtg acgctggtcg ccgcctcggt gctctatacg gccgcgctgg ggctgatcta
     1381 catgcaacgc cagcaactca tcgaagcgtt gcagcccccg gaagagcagg aagcggccga
     1441 cggcaccacc ttgccggcga tcaccacgcc gacacccatg ctggaagtgt tccccccgac
     1501 gccggagccg acctcgctga ccccggcccc cacggcgacg gtgacgccga ccgtctgccg
     1561 cgcggtggtc gaaggaacag gcggcaacgg catttatctg cgcgacgccc cgcaggggga
     1621 acaactcgtg atcctgcctg acgggacgct gctgacggtg ctggaagacg ccccggtcga
     1681 ggcgggcggt tttgtatggc gcaaggtgcg cgccgttggc ggcgaggaag gttgggccgc
     1741 tcaggacttc ctgaccattc gcgccccttg tgggagtacg gattaggaag ccgcagatgc
     1801 cctcccatga ttatcggtat tgacgccagc cgcacgacga ccagccaccg caccggcacg
     1861 gaagcctacg cctatcggct gatcgacgcg ctcctgcccc cggctgccga ccgcggccat
     1921 cgcctccgtc tttacttcaa ccaatcgccc gccgctgatc tgtttccggc ttcggaacac
     1981 gtcgaacgcg taatcatccc ccagcgccga ctatggacgc
//
LOCUS       DAOMQG010000520         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21728_length_2014_cov_7.259826, whole genome shotgun sequence.
ACCESSION   DAOMQG010000520 DAOMQG010000000
VERSION     DAOMQG010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21728_length_2014_cov_7.259826"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1449
                     /locus_tag="PLC06_16755"
     CDS             166..1449
                     /locus_tag="PLC06_16755"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRO92153.1"
                     /translation="MSSKREPGRQIIVVCLLGLAAILYLRLIPFDFIRADHIPFERIT
                     WRPLTFRDVPLNILLFIPFGFGLTGLLVRRGRQSGIAGRVVLIGVLLSAALEATQLFM
                     PDRAPSFADVAANAVGALLGYGLYRAWEMGFGVALQRYATRRNLLIGTTLYACGVVLL
                     TGYLYRSVRLSNWDTSFPLIVGNEAVGKRQWSGWMGSLALEAGMSGDSGVLIVYDLKG
                     DAPFAAVHQRPAAPPLDWRAGPVTPQDGEGVNVGPGEWLATDEPVTDFSSTARDADAF
                     SVEMTVASADPAQRGPARIVSVSADAERRNITIGQEYDALIIRLRTPAGGENGQKPEL
                     LIPGVFAGNRRRHIQVGYDVPMLRAKVDGRGYSLSLAPGLAFFPGFTTENRRQVLMTG
                     DPHRYDWVYWGIIVGPAVLIFGGLAIAKRLSERDQ"
     gene            complement(1443..>2014)
                     /gene="pgl"
                     /locus_tag="PLC06_16760"
     CDS             complement(1443..>2014)
                     /gene="pgl"
                     /locus_tag="PLC06_16760"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRO92154.1"
                     /translation="FFWGDERLVPPDDPGSNYGEAGEILLRPLNIPEQNIHRIRGELS
                     PQKAAAGYAALLRDWAAEHDPGAPNPWPRFDLVLLGLGEDGHTASLFPGSPVAVDAPV
                     IAVTADYQGRPAGRVTLTPLVFNDARDVNFLVSGKNKADAVYHTFYTGDPVRYPAQRI
                     RPANGDVTWLLDRAAATRLPKFRRDKDRY"
BASE COUNT          326 a          645 c          669 g          374 t
ORIGIN      
        1 atgcctgggt cgacgcgctg caagagatca ccgactggtt caaggctttt caggcgggga
       61 aagacatgca cgatgtgacg cgcgcccaca tcctgaccta ttccgaccac gcccgcgaac
      121 tggggctgga ttgggcggcg ggctgatcca ctccgacgtg ccgccatgtc cagcaaaaga
      181 gagccgggtc gccagatcat cgttgtttgc ctgttggggc tggccgccat tctctatctg
      241 cgcctgatcc cgttcgactt catccgcgcc gaccacatcc cgttcgagcg gatcacctgg
      301 cgcccgctaa cctttcgcga cgttccgctc aacatcctgc tcttcatccc gttcggcttc
      361 gggttgaccg ggttactggt gcgtcgtggc cgacagagcg ggatagccgg ccgcgtggtg
      421 ttgatcggag tgctgctctc ggcggcgctg gaagcaacgc agctattcat gcccgaccgc
      481 gccccgtcct tcgccgatgt ggccgccaat gccgtcggcg cgctgctggg ctatggcctg
      541 tatcgggcct gggaaatggg cttcggtgtc gcgctgcaac gctacgccac ccggcgcaac
      601 ctgctgattg ggacgacgct ctacgcctgc ggcgtggtac tgctcacggg ctatctgtat
      661 cgcagcgtcc gcctcagcaa ctgggatacc tcattcccgc tgattgtcgg aaatgaggcg
      721 gtgggcaagc ggcaatggag cgggtggatg ggatcgctgg cgctggaagc cgggatgagc
      781 ggcgactccg gcgtcctcat tgtatacgat ctgaagggtg atgcgccttt cgcggccgtt
      841 caccagcgcc ctgccgcgcc gccactcgat tggcgcgcgg ggccagtgac gccacaggac
      901 ggcgaaggag tcaacgtcgg cccaggcgaa tggctggcga ccgatgagcc ggtgacggat
      961 ttttcatcga cggcaaggga tgccgacgcc ttcagcgtcg agatgaccgt cgcctcggcc
     1021 gacccggcgc aacgcggccc ggcgcgcatc gtctccgtct cggccgacgc cgagcggcgc
     1081 aacatcacta tcggccagga gtatgacgcg ctgatcatcc gcctgcgcac cccggccggc
     1141 ggcgagaatg gccagaagcc ggaactgctg attccgggcg tcttcgccgg caatcggcgg
     1201 cgacatatcc aggttgggta cgatgttccg atgctgcggg cgaaggtcga cggccgcggc
     1261 tattccctgt cgcttgcgcc ggggctggcc ttcttccccg gcttcaccac ggagaaccgg
     1321 cggcaggtcc tcatgacggg cgacccccac cgctatgatt gggtctattg ggggatcatc
     1381 gtcgggccgg ctgtcctcat atttggtggg ttggcgattg caaagcggct ttcagaaagg
     1441 gatcaataac gatctttgtc ccgtcggaat ttaggcaatc tcgtcgccgc cgcgcgatcc
     1501 agcagccagg tgacgtcacc gtttgccggg cgaatgcgct gcgccgggta gcgaacgggg
     1561 tcaccggtat agaacgtatg gtagactgcg tcggccttgt tcttcccgga gacgaggaaa
     1621 ttcacgtcgc gcgcatcatt gaacaccaat ggcgtcagcg tcacacggcc ggccgggcga
     1681 ccctgatagt cggccgtgac ggcgatcacc ggagcgtcca ccgcgacggg cgagccgggg
     1741 aacagcgacg ccgtgtgccc gtcctcgccc agtcccagca gcacgaggtc aaatcgcggc
     1801 caagggttcg gcgcgccggg gtcgtgttcg gccgcccagt cccgcagcag ggcggcatag
     1861 ccggccgcag ccttttgggg cgatagctcg cccctgatcc ggtggatatt ctgctccggt
     1921 atgttcagcg gcctgagcaa aatttcgccg gcctcgccgt agttgctgcc ggggtcatcg
     1981 ggcgggacga ggcgctcgtc gccccaaaag aagt
//
LOCUS       DAOMQG010000521         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21929_length_1999_cov_12.985082, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000521 DAOMQG010000000
VERSION     DAOMQG010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21929_length_1999_cov_12.985082"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLC06_16765"
     CDS             complement(<1..638)
                     /locus_tag="PLC06_16765"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HRO92155.1"
                     /translation="MELWIGLLIGLVVGAAIAVLIARTVMRPRIAEMVATAEAELAEK
                     RETAEREAENILRTSRESAQDIRLEADKAIERRYQDLARAEERVDRRGANQDQQAKTL
                     EQREQLLNKRQSRIDKRQNELGDLETEHRQKLEETAAMTTDEARAVLIQQVEKEAQQD
                     LARVMREIDNRAKETADEQARKIIALAIQRLASEQVADMVVSVVPLPSDEMKG"
     gene            complement(651..1280)
                     /locus_tag="PLC06_16770"
     CDS             complement(651..1280)
                     /locus_tag="PLC06_16770"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRO92156.1"
                     /translation="MGTITALTAQVKNPDRVNVFIDGTFTLGLAMSVAAGLRTGQEIS
                     RSELEKLDLRDEIHRARERVLRLLARRPYSSSEIARYLRRHQIDGEIIQNVIDDLTEA
                     KLIDDDAFAVYWVEQRETFRPRSRLALRQELNQKGVDRETVAEALSEVDEIDAARRVA
                     QKQAGRWRGLPETEWRAKLTRYLMRQGYPYDIVSEIVTEVWLANQPDEE"
     gene            complement(1775..>1999)
                     /locus_tag="PLC06_16775"
     CDS             complement(1775..>1999)
                     /locus_tag="PLC06_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HRO92157.1"
                     /translation="IMYNEGISRTGDVLDLAIESNLLDKRGAFIRYGDTLLGQGRENA
                     KTFLAQNPAMLAELESQIRQSAGLPAAQVR"
BASE COUNT          379 a          580 c          538 g          502 t
ORIGIN      
        1 cccttcatct cgtcgctcgg caacggtacg acggagacga ccatgtcggc cacctgttcg
       61 ctggccagcc gttggatcgc cagggcgatg atcttgcggg cttgctcatc ggccgtttcc
      121 ttggcgcgat tgtcgatctc gcgcatcacc cgtgccagat cttgctgggc ctctttttcc
      181 acctgctgga tgaggacagc gcgcgcttcg tcggtcgtca tggcggcggt ctcttccagt
      241 ttctggcgat gttccgtttc cagatcaccc agttcgtttt gtcgtttgtc gattcgactc
      301 tgacgtttgt tgagcaattg ttcgcgctgt tccagggttt tggcttgctg gtcctggttg
      361 gcgccacgac ggtcgacgcg ctcctcggca cgtgccaggt cctgatagcg tcgttcaatg
      421 gccttgtcgg cctcgaggcg gatatcctgt gccgactcgc gggaagtgcg gaggatgttt
      481 tccgcttccc gctcggccgt ttctcgcttc tcggccagtt cggcctcggc tgtggcgacc
      541 atctcggcta tacggggccg catgacggtc cgggcaatga gcacggcgat ggccgctccc
      601 accaccaggc caatcagcag gcctatccat agttccatga taatttctcc ttattcttcg
      661 tcaggttgat tggcaagcca gacttctgtg acgatttcac tgacgatatc gtaggggtat
      721 ccctgtctca ttaaatatct cgtcagtttg gcgcgccact ccgtctccgg tagtccgcgc
      781 catcgcccgg cttgcttctg cgcgacccgc ctcgcggcgt cgatctcgtc cacttcggac
      841 agagcttcgg cgacggtttc acgatcaaca cctttctgat tcaactcttg ccgcaaggcc
      901 aggcggctgc gcggccgaaa ggtctcgcgc tgttccaccc agtagacggc aaaggcgtcg
      961 tcgtcgatca acttcgcttc ggtcaggtcg tctataacat tttgaatgat ttcgccatcg
     1021 atttgatggc ggcgcaaata acgcgcaatt tcggacgaac tgtagggacg gcgagccaac
     1081 aggcgcaaca cgcgctcgcg ggcgcgatga atttcatcgc gcaaatccag cttttccagt
     1141 tcactccggg agatttcctg ccctgtcctc aagccggcgg caacagacat ggccagcccg
     1201 agggtgaatg tgccgtcaat gaacacattg acccgatccg ggtttttcac ctgcgccgtc
     1261 agggcggtaa tcgttcccat aatgtaaaaa caaaagccgt ggctacaagc cacggccggc
     1321 attcaggcgc ttcagatctt gcgctcagtt gtcaataaag ccccctgata atgggggaag
     1381 tccaattagc gacgatgtat gaaaatgcgg ctacagttcc cgatgcggtt ggttgcgaac
     1441 actatgtact tgatatagcg tgctgttggg gcacgcccgg cggattcatc atcaagttcc
     1501 catataaact ccatcgatat tgaagacagg cacctgggtg ccaactgggt tcaatatcga
     1561 tgaggtgatt cattctaatc aacaacaggt ttcctgtacg caaatttcag gttcaaacgc
     1621 gaccagctaa ctggatttta ccttatcgac ccttaatgcc atcggcgccg cttgtgcgga
     1681 cacctttctc tagctgtggc agtaagccgt cagtattaat tcattttata aacttattgc
     1741 gcgtttccaa ggaacgcgca atgagttcgc tcgatcagcg aacttgtgcg gccggcagac
     1801 cggcagactg gcgtatctgt gattccagct cagccagcat ggccggattt tgggccaaaa
     1861 aggtcttggc gttttctcgt ccctgaccca aaagtgtatc tccatagcgg atgaacgcac
     1921 cgcgcttgtc gagcaggttg ctttcaatcg ccaggtcgag gacatcgccg gtcctggaga
     1981 tcccctcatt gtacatgat
//
LOCUS       DAOMQG010000522         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_21957_length_1997_cov_9.520082, whole genome shotgun sequence.
ACCESSION   DAOMQG010000522 DAOMQG010000000
VERSION     DAOMQG010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21957_length_1997_cov_9.520082"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..860
                     /gene="cofC"
                     /locus_tag="PLC06_16780"
     CDS             195..860
                     /gene="cofC"
                     /locus_tag="PLC06_16780"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HRO92158.1"
                     /translation="MTIWAIIPVKPLHDSKRRLAHLLSAEARAELIHHFLDNLLATLN
                     DVPGIDRVLVVTGDAEATALAQNHGAAVLLETEPSGLNEAVRRGAALAVRQGATAVLI
                     LPADLPFARVEDIEQMLAPLPTAPLPTDLLTTAPLPTTHHPLLAITGDETEEGTNALL
                     LAPPGDFTFHYGPGSYHAHLNEAAARGRTTCIINAPGLRFDLDTEGDWLAYCGCLIQA
                     GDE"
     gene            959..1966
                     /locus_tag="PLC06_16785"
     CDS             959..1966
                     /locus_tag="PLC06_16785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRO92159.1"
                     /translation="MSRDRLALYLQDAHSLQEGMELSQYAEANGFEAVWQAESRLVRD
                     AIVPMAAFAAVTDHIKVASGVINNWTRNIGLLAATFLTLDDLAPDRVICGIGAWWDPL
                     AQKVGIDRRKPLLAMRETVEVLKRLLNMETVTFHGEFHYVDGITLDVVHGRKTPRNVP
                     IYIGATGMKMMEMAGEIADGVCLNYLVNPKYNAEAMDALERGAKKAGRNIDDIDRPQL
                     VICSVDNDRKHALNMARKMMTQYLGQQPHLMKASGVSQELLDEIHQVLTWPATEEEIE
                     GAMHLVPDDVVQMCTASGSPAEVKAKVREYIDSGATCPILYPLGDPKLMIDIFSDGYQ
                     D"
BASE COUNT          404 a          704 c          564 g          325 t
ORIGIN      
        1 aggggcgaca ggtcgatgat agcgattagg tcatcatgct ttgcgcatta aggcctgatt
       61 ataggccaaa caccggagga atacagcagg agacttagcg ggcgatcatc tgaggagggt
      121 ggggaagtac cctttttcca cttcaccttc acttctttac actcatccac caccatcggg
      181 tataatcgcc cgcaatgacc atctgggcca tcattcccgt caagcctctc cacgacagca
      241 agcgtcgttt ggcgcatctg ctctcggccg aggcgcgcgc cgaactcatt caccattttc
      301 tcgataacct gctggcgacc ctgaacgacg tgccgggcat cgaccgcgtg ctggtcgtga
      361 cgggcgacgc cgaggcgacg gccctggccc agaaccacgg cgcggccgtc ctgctcgaga
      421 cggaaccgtc gggcctgaac gaagccgtcc ggcgcggtgc ggcgctggcc gtccggcaag
      481 gcgcgacggc cgtcctcatc ctgcccgccg acctgccctt cgcccgcgtc gaggatattg
      541 agcaaatgct cgccccactc cccactgccc cactgcccac tgacctactg accactgccc
      601 cactccccac cacccaccac ccgctacttg ccatcaccgg cgacgagacc gaagaaggca
      661 ccaacgcctt gctgctggcc ccgcccggcg acttcacttt ccactacggc cccggcagct
      721 accacgccca tctgaacgag gcagccgcgc gcggccgaac gacctgcatc atcaacgccc
      781 ccggcctgcg cttcgatctg gataccgagg gcgactggct ggcatattgt ggttgcttaa
      841 ttcaggcagg cgatgaataa taagcaaccg ccgccgaccg accaccgatt gatatcgatc
      901 aactgtggct gggcgtcggc cgacaatttc cccaccccca cccaaaggag tcaaaactat
      961 gtcccgtgat cgcttagccc tctatctaca agacgcccac tcattgcagg aaggcatgga
     1021 gttgtcgcaa tatgccgagg ccaacggctt tgaagccgtc tggcaggccg agagccgcct
     1081 cgtgcgcgac gccatcgtgc ccatggccgc cttcgccgcc gtcaccgacc acatcaaggt
     1141 cgccagcggc gtcatcaaca actggacgcg caacatcggc ctgctggccg ctaccttcct
     1201 gacgctcgac gacctggccc ccgaccgcgt catctgcggc atcggcgcat ggtgggatcc
     1261 gctggcccaa aaggtaggca tcgaccgccg caagccgctg ctggccatgc gtgagacggt
     1321 cgaagtgctg aagcgactgc tcaacatgga gaccgtcacc ttccacggcg agttccacta
     1381 tgtcgacggc atcaccctcg acgtcgtcca cggccgcaag accccgcgca acgtgcccat
     1441 ctacatcggc gcgacgggca tgaagatgat ggagatggcc ggcgaaatcg ccgacggcgt
     1501 ctgcctcaac tacctggtca accccaagta caacgccgag gcgatggacg cgctggagcg
     1561 cggggccaag aaggccggcc gcaatatcga tgacatcgat cggccgcagc ttgtcatctg
     1621 ctccgtcgac aacgaccgca agcacgcgct caacatggcc cgcaagatga tgacccaata
     1681 tctgggccag cagccgcacc tgatgaaggc cagcggcgtc agccaggagc tgctcgacga
     1741 gatccaccag gtgctcacct ggccggccac cgaggaggag atcgaaggcg ccatgcacct
     1801 tgtgcccgac gacgtggtgc agatgtgcac ggccagcggc tctccggccg aggtgaaggc
     1861 caaggtgcgc gaatacatcg acagcggcgc cacctgcccc atcctctacc cgctgggcga
     1921 cccgaagctg atgatcgaca tcttcagcga cggctatcag gattagggct gacccgactc
     1981 ttgtccgccg ccgacgg
//
LOCUS       DAOMQG010000523         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22071_length_1989_cov_4.920889, whole genome shotgun sequence.
ACCESSION   DAOMQG010000523 DAOMQG010000000
VERSION     DAOMQG010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22071_length_1989_cov_4.920889"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="PLC06_16790"
     CDS             complement(<1..1784)
                     /locus_tag="PLC06_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRO92160.1"
                     /translation="MTNEEGPATTNHPVPPSPLLPFSPAPASRPLDRFIPPELLAKLS
                     EARGSAGERRVVTLLFCDIQGSTALAEAHDPEEWTEIVNAAFEQMVRPIYKYEGTVAR
                     LMGDGLLAFFGAPIAHEDDPRRAVLAALAIVEGVHAWRATLPPAYAGLDVRVGINTGL
                     VVVGAVGSDLRLEYSAIGDAINLAARMEQTAAPGTVQIAEDTWRLVADQFEVEPLGLI
                     AVKGKTQPVAAWRVLRRGMDARRTAGGMRAPLIDRRMAWEALRQGIDDLGNGRGSVVF
                     LTGEAGLGKTRLIDEVAEQLLPELSPPGRIFDASAVSYETNQPYGLLVRLLRRPMNIM
                     SGDSPEQMRAAISATFADAEDRALVETLFGVADPGSGQEIGGEGFADRLDTVLERYWQ
                     AQAADGPLVLALDDLQWLDTSSAERLTRLVRLTEDTPILFIGAMRRDRRSPAWTLKET
                     VARELPHRQTEVNLNPLNDGESRALLAGLLAVAEPPRLLSSLILDKAEGNPLFLEEVV
                     RHLIERGDLRRAAEASGEWQVTPTAITLPDSLQSLLTARFDRLDESARRVLQIAAIIG
                     RHFYRTPLAALVDDPARLDSCLLELQRL"
     gene            complement(1774..>1989)
                     /locus_tag="PLC06_16795"
     CDS             complement(1774..>1989)
                     /locus_tag="PLC06_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92161.1"
                     /translation="GNRAHDNLLTLWRKQPGRRGFLPQLRPSFIGRLPPLRRGRHPQR
                     QLLRQLRFPSQPTRLDGNYELRITNDE"
BASE COUNT          329 a          643 c          702 g          315 t
ORIGIN      
        1 tccagccgtt gcagctccag caggcagctg tcgaggcggg cggggtcatc gaccaaggcc
       61 gccaacggcg tgcggtagaa atgacggccg atgatcgccg caatttgcag cacccgccgg
      121 gccgattcgt ccagccggtc gaagcgcgcc gttagcagcg attgcagcga atcgggcagg
      181 gtgatggctg tgggggtcac ctgccattcg ccggacgcct cggccgcccg ccgcaagtcg
      241 ccgcgctcga tgagatggcg cacgacttcc tcaaggaaga gggggttgcc ttcggccttg
      301 tcgaggatga gcgacgacag caagcgcggc ggctcggcta ccgccagcag cccggccagc
      361 agggcgcggc tttcgccgtc attgagcgga ttcagattga cctcggtctg gcgatggggc
      421 aattcgcggg ccaccgtctc cttgagcgtc caggccggcg agcggcggtc gcggcgcatg
      481 gccccgatga acaggatggg cgtgtcttcg gtcaggcgaa ccagccgcgt caaccgttcg
      541 gccgaggacg tatcgagcca ttgcaggtcg tcgagagcca gcaccagcgg cccatcggcc
      601 gcctgtgcct gccagtagcg ctccagcacg gtgtccagcc ggtcggcgaa gccttccccg
      661 ccaatttcct gcccgctgcc gggatcggcc acgccgaaca gcgtttccac aagggcgcgg
      721 tcttcagcgt cggcaaaggt ggcgctgatg gccgcgcgca tttgctccgg cgaatcgccc
      781 gacatgatgt tcatcggccg ccgcagcagc cgaacgagca agccataggg ctgattggtc
      841 tcgtaggaaa cggccgaggc gtcgaagatg cgtccgggcg ggctgagttc cggcagcagc
      901 tgttcggcga cctcgtcgat gaggcgggtc ttgcccagcc cggcttcgcc ggtcagaaac
      961 acgacgctgc cgcggccgtt accgagatcg tcgatccctt gccggagcgc ctcccacgcc
     1021 atgcgccggt cgatgagcgg agcgcgcatg cctccggcgg tgcggcgagc atccatcccg
     1081 cgccgcaaaa cgcgccacgc ggcgacgggt tgggtcttgc ctttcacggc aatcaggccg
     1141 agcggctcaa cttcaaactg atcggccacc aggcgccagg tatcctcggc gatctgcact
     1201 gtgccggggg cggccgtctg ctccatgcgc gcggccagat taatggcgtc gccgatggcg
     1261 ctgtattcca gccgcaagtc gctgcccacc gcgccgacga cgacgagacc ggtgttgatc
     1321 cccacgcgca cgtcgagacc ggcataggcc ggcggcagcg tcgcgcgcca ggcgtggacg
     1381 ccctcgacga tggccagcgc ggccagcacc gcccggcgcg ggtcgtcctc gtgggcgatg
     1441 ggcgcgccga agaaggccag caagccgtcg cccatgagcc gggccacggt gccctcatat
     1501 ttatagatcg gccgcaccat ctgctcgaag gcggcgttga cgatctccgt ccattcctcc
     1561 gggtcgtggg cttcggccaa cgcggtggaa ccctggatgt cgcaaaagag cagtgtgacc
     1621 acgcgccgct cgccggccga accgcgcgcc tcgctcagtt tggccagcag ttcgggcggg
     1681 atgaagcggt cgaggggccg ggaagcagga gcaggggaga aagggagaag gggagaaggg
     1741 gggacggggt gattggttgt agccggccct tcttcattcg tcattcgtaa ttcgtaattc
     1801 gtaattccca tccaggcgcg tgggctgaga ggggaaccgc aattgtcgca gaagttggcg
     1861 ttgggggtgg cggccgcgcc gcagcggggg caggcggccg ataaaagacg gccgcaattg
     1921 cggcagaaag ccgcgtcgtc ccggttgttt gcgccacagt gtgagcaggt tgtcatgcgc
     1981 ccggtttcc
//
LOCUS       DAOMQG010000524         1987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22101_length_1987_cov_8.507246, whole genome shotgun sequence.
ACCESSION   DAOMQG010000524 DAOMQG010000000
VERSION     DAOMQG010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22101_length_1987_cov_8.507246"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLC06_16800"
     CDS             <1..499
                     /locus_tag="PLC06_16800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRO92162.1"
                     /translation="FFRDRGLDVTCTDLSPEMVRLCRQKGLTAHVMDFLHLDFPPVSF
                     DAVFALNCLLHVPGVDLDRVLAAVQQLLRPGGLFFYGVYGGHSFEGVWPEDHHDPKRY
                     FVFYPDDELRRRVAERFDEVSFRAIQLEDDRESHFQSLILRPRSFLAPTDGISNAGIK
                     GSSAV"
     gene            533..1312
                     /locus_tag="PLC06_16805"
     CDS             533..1312
                     /locus_tag="PLC06_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRO92163.1"
                     /translation="MYVMGDIHGQIDKMRAALRAAELLDDEDRWIGGATTLWFMGDYF
                     DRGPDGIATIDLIRSLQAEAAAAGGRVGALIGNHDVLILAAQRFGDRPSGGPGGTFLS
                     SWRYNGGEETDLTRLTADRAGWLRALPAMALDGGRLLAHADATFYQVYGRSVAAVNDH
                     FRAILQSGDAQAWDQLLDYFSQRRAFQDRDLGPARVGRILGLYGGRQLVHGHTPIAKL
                     MGCRPEDVTQSLTYAGGLAIDVDAGMYLGGRGFVTCLSNID"
     gene            1331..>1987
                     /locus_tag="PLC06_16810"
     CDS             1331..>1987
                     /locus_tag="PLC06_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRO92164.1"
                     /translation="MRIAIFSDVHGNLSALEAVLADMESRSPDLVVFAGDLCLFGPRP
                     AECLRLVRGQRLPSVIGNTDEWIMGMDSPPEKHAAELQWTAAQLAPDEMDWLHRLPFS
                     LRISPNDAPNGHDLLIVHANPQDVNEIVFPSIVDQQTRWGRIRQNDEDLETLMGKVDA
                     AAIAYGHLHIPGVRQWGDVLLANISSVNMPGDGNGRAKYGLLEWRDGRWEIAHHRVAY
                     D"
BASE COUNT          350 a          630 c          604 g          403 t
ORIGIN      
        1 tttcttccgc gaccggggcc tcgacgtcac ctgcaccgac ctctcgccgg agatggtgcg
       61 cctctgccgc caaaaagggc tgaccgctca cgtgatggac ttcctgcacc ttgattttcc
      121 tccggtctcg ttcgacgccg tcttcgctct caattgcctg ctccacgttc ccggcgtcga
      181 tctggatcgg gtgctggcgg ccgtccagca gctgctgcgg ccgggcggct tgtttttcta
      241 cggcgtctac ggtggccatt cgttcgaagg agtttggccg gaagatcacc acgatcccaa
      301 acgctacttc gtcttctatc ctgacgacga actccgccgg cgggtagccg agcgattcga
      361 cgaagtgtcc tttcgcgcca tccaactcga ggatgatcgc gagtcacatt ttcaatcgct
      421 catcctgcgg ccaaggtcat tcctcgcccc gacggatggc atatccaatg cgggcataaa
      481 aggatcatca gccgtgtaat cggccgatcc cctttgatga ggtgacaaac cgatgtacgt
      541 catgggcgac atccacggcc agatcgacaa gatgcgcgcc gcgttgcgtg cggccgagtt
      601 gcttgacgac gaagaccgct ggatcggcgg ggcgaccacg ctctggttca tgggcgatta
      661 tttcgaccgt gggccggacg gcatcgccac catcgacttg attcgctcgc tgcaggcgga
      721 ggcagcggcc gcgggcgggc gcgttggcgc gctaatcggc aaccacgacg tgctgatcct
      781 ggccgctcag cggtttggcg atcggccgag cggtggcccc ggcggcacgt tcctgtccag
      841 ttggcgatat aacggcggcg aagaaactga tctgacccgg ttgacggccg atcgggccgg
      901 ctggctgcgc gcactcccgg ccatggcgct cgacggcggc cgcctgctgg cccatgccga
      961 cgccacgttt tatcaggtct acggccgcag cgtcgccgca gtcaatgatc acttccgggc
     1021 catacttcag agtggtgatg cgcaagcctg ggatcagttg ctcgactact tctcacagcg
     1081 tcgcgccttc caggatcgcg atctggggcc ggcgcgggtc ggccgcatcc tgggcctcta
     1141 cggtggccgg caacttgtcc acggccacac gcctatcgcc aagttgatgg gttgccggcc
     1201 ggaggatgtc actcaatcgc tgacctatgc cggcggactg gccatcgacg tggacgcggg
     1261 gatgtatctt ggcggccgcg gctttgtcac ctgtctaagc aatatcgatt gattttgcgg
     1321 gagttttatc atgcgaattg caatattttc cgacgtccac ggcaatctca gcgcgctgga
     1381 ggcggttctg gcggatatgg aaagccggtc acccgacctc gtcgtatttg cgggcgattt
     1441 atgcctgttc ggcccgcgcc cggccgaatg cctgcgcctg gtgcgcgggc agcgactgcc
     1501 gtccgtcatc ggcaacaccg acgagtggat catgggcatg gattccccgc cggagaaaca
     1561 cgcagccgag ctgcaatgga cggccgcaca gcttgcgccc gacgagatgg attggctgca
     1621 tcgcctgcct ttctccctgc gcatctctcc caatgatgcc ccgaacggcc atgacctgct
     1681 catcgttcat gccaaccccc aagacgtaaa cgagattgtg tttccgtcca ttgttgatca
     1741 gcaaacgcga tggggcagga ttcgtcagaa cgacgaagac ctcgaaacgc taatgggcaa
     1801 ggtcgacgca gcagcaatag cctatggcca cctgcacatc cccggcgtac gccaatgggg
     1861 cgatgtcctg ctcgccaata tcagctcggt caatatgccc ggcgacggca acggccgcgc
     1921 caaatatggc ttgctggaat ggcgcgacgg ccgctgggag atcgcacacc atcgagtcgc
     1981 ttatgat
//
LOCUS       DAOMQG010000525         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22488_length_1959_cov_8.307773, whole genome shotgun sequence.
ACCESSION   DAOMQG010000525 DAOMQG010000000
VERSION     DAOMQG010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22488_length_1959_cov_8.307773"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1667
                     /locus_tag="PLC06_16815"
     CDS             24..1667
                     /locus_tag="PLC06_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HRO92165.1"
                     /translation="MVDFLVEEPLLLLFLVSGLGYALGNVKVKGISLGVAAVLFVGLA
                     FGALSPEMRLPTVLVDLGLIVFVYTIGLSSGPGFFASFRRKGLRDNAFVFLMLILAAL
                     MTVAAAGLFGLRATVAAGMYSGSLTNTPALAGILETIARVAPAGQLIVLETEPVIGYS
                     VAYPMGVIGLLIAIYTMQRVYGIDYKTEARALHQYHVVEQDLYARTALVTNPDVAGVA
                     VSDLFRRHQWDVVLSRIRRGEELTLATGDTTFVLGDEVTLVGTADEVDRAVAELGEPA
                     GKQLELDRSEYDYRRIFVSNPELAGRSLSELDLPREYGAIVSRVRRGDIDLLAHGDTV
                     LELGDRVRVVARRTDQPRLAALFGDSYKALSEVNLLSLSVGLALGVLIGLIPIPLPGG
                     VVITLGLAGGPLLVGLVLGALRRTRGIVWTIPYSANLTLRQIGLILLLAGIGVRSGYT
                     FLTNFTASGGVQVFFAGAFITMAIAFLTLIIGHKVLKVPFGMLTGMLSALGTQPAALG
                     FSLQQADNELPNLGYALVFPVATITKVVVAQLLLIFLSG"
BASE COUNT          296 a          593 c          682 g          388 t
ORIGIN      
        1 taaaggacaa ccctatgcag cccatcgttg attttctggt ggaagaaccg ctcttgctgc
       61 tgtttctcgt ctccgggctg gggtatgcgc tcggcaacgt gaaggtgaag ggcatcagcc
      121 tgggggtggc ggcggtgctg ttcgtcgggc tggcgtttgg cgcgctcagc ccggagatgc
      181 gcctgcccac ggtgctggtc gatctggggc tgatcgtctt cgtctacacc atcggcctca
      241 gcagcgggcc gggtttcttc gcctcgttcc ggcgcaaggg gctgcgcgac aacgccttcg
      301 tcttcctcat gctcatcctg gcggcgctga tgaccgtggc cgccgccggg ctgttcgggc
      361 tgcgggccac ggtggcggcg ggcatgtact cgggcagcct gaccaacacc ccggcgctgg
      421 ccggtattct ggagaccatc gcccgcgtag ccccggccgg ccaactgatc gtgctggaga
      481 cggagccggt catcggctac tcggtggcct atccgatggg ggtcatcggc ctgctcatcg
      541 ccatctacac catgcagcgc gtctatggca tcgactacaa gaccgaagcc agggcgctgc
      601 accagtacca cgtcgtcgag caagacctct acgcgcgcac ggcgctggtc accaaccccg
      661 acgtggccgg cgtggccgtc agcgatctgt tccgccggca ccagtgggat gtggtgctgt
      721 cgcgcatccg gcgcggcgag gagctgaccc tggccaccgg cgacacgacc ttcgtcctgg
      781 gcgacgaggt gacgctggtg ggcacggccg acgaggtgga tcgggccgtg gccgagctgg
      841 gcgagccggc ggggaagcag ctggagctgg accgcagcga gtacgattac cggcgcatct
      901 tcgtctccaa cccggagctg gccgggcgca gcctcagcga gctggacctg ccgcgggagt
      961 acggggcgat tgtctcgcgg gtgcggcggg gggacatcga cctgctggcc cacggcgaca
     1021 cggtgctgga gctgggcgac cgggtgcgcg tcgtggcccg gcgcaccgac cagccgcggc
     1081 tggcggccct cttcggcgat tcctacaagg cgctgagcga ggtgaatctg ctgtcgctga
     1141 gcgtggggct ggcgctgggg gtgctcatcg gcctcatccc catccccctg cccggcgggg
     1201 tggtcatcac gctggggctg gccggcgggc cgctgctggt ggggctggtg ctgggggcat
     1261 tgcgacgcac gcgcggcatc gtctggacca tcccctatag cgccaatctg acgctgcggc
     1321 agatcgggct gatcctgctg ctggcgggga tcggtgtgcg ctccggctac accttcctga
     1381 ccaacttcac ggccagcggg ggcgtgcagg tgtttttcgc cggggcgttc atcacgatgg
     1441 cgatcgcttt cctgacgctg atcatcggcc ataaggtgct gaaggtgccg ttcgggatgc
     1501 tgaccgggat gctgtcggcg ctgggcacac agccggcggc gctggggttc agcctgcagc
     1561 aggcggacaa cgagctgccc aatctggggt atgcgctggt cttcccggtg gcgacgatca
     1621 cgaaggtggt ggtggcgcag ttattgttga tttttttatc gggatgagtc catttcgggt
     1681 acgggcgagg caaccgggga actcgtttgt gattaagcgc ttttgcatgt taacaaaata
     1741 atcgcgtatc aatcaaacgc gggatgaatc ccgcggctgt tatgccaaac cgactgaagt
     1801 cggttgccgc ctctgccggc aaccggtttc aaccggtttt gtctatcagc agcggatttc
     1861 aatccgccga ggcgtatacg tgattaattt gtaaatattc aacagcgccg tcgccggtta
     1921 tctcgcccat tcgtttgtag caggctatag taatagcaa
//
LOCUS       DAOMQG010000526         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22555_length_1955_cov_8.666842, whole genome shotgun sequence.
ACCESSION   DAOMQG010000526 DAOMQG010000000
VERSION     DAOMQG010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22555_length_1955_cov_8.666842"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="PLC06_16820"
     CDS             complement(<1..1938)
                     /locus_tag="PLC06_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO92166.1"
                     /translation="MPAPDNEDTTAGGKSYHLSGDFRGAIVNIESTFVNSAAAQDIEG
                     LAPEAGDPPYKGLQYFDEADADHFLGRETLTAKLVGQLRETRFLAVIGASGSGKSSVV
                     RAGLVPALRRGKPLADGALPPTGSDSWLIRVMTPTANPLDALATALLPDGEPAAVAAL
                     RDRLGAGPTALVDALPDALGKSQPKLLLIVDQFEELFSLVRHEADRRAFIDNLVTAAG
                     GDQPILIVIVLRADFYAHCAQYEGLRELVSRHQEYIGAMSREELFRVIVLPAARDKWK
                     LQEGLVELMLDDVGDEPGALPLLSHALLETWKRRRGRTMTLSGYKESGGVRGAIAKTA
                     ETVFRQRLTPEQQPIARMIFMRLTELGESADSDTPDTRRRAQFSELITRSTDAATLDA
                     VLSILIDSRLVMTDVIPPGETKVVEVAHEALIREWPTLRDWLNQDREGLIRHRQLTAD
                     VNDWLSLNRDPGALYRGAKLEQALTWTAKPPDPLNVVEQEFLDASRAAAEEDARQAER
                     LAKASRNQRILVGVAAALLLGVVVAILFNMGVFSPPAKAMTGDFKVAVAQFAMLDESG
                     QLADASNDGGRRIAERIGDDLTEESGADLSVWFDGPASKGHVPVGIAGEGIDGAIDPA
                     ALAEKLNADMIVYGTVKPDGEF"
BASE COUNT          350 a          641 c          606 g          358 t
ORIGIN      
        1 aaattcgcca tccggtttca ccgtgccgta aacgatcatg tcggcgttga gcttttcagc
       61 caacgctgcc ggatcgatcg cgccgtcgat cccctcaccc gcgatgccga ccggcacgtg
      121 acctttactc gccgggccgt cgaaccagac gcttaaatcc gccccggact cttccgtcag
      181 atcgtcgccg atacgctcgg cgatgcggcg gccgccgtca ttgctcgcat ctgccaactg
      241 cccgctttcg tcgagcatgg caaactgggc cacggccacc ttgaaatcgc cggtcatcgc
      301 cttcgcggga gggctaaata cacccatgtt aaagagtatg gcgaccacga cccccaacaa
      361 gagcgcggcg gctacaccca ccaggatccg ttgattgcgg gaagcctttg ccagtcgttc
      421 ggcttgccgg gcatcttcct cagccgcggc gcggctggca tccaggaact cctgttcgac
      481 cacgttcaac ggatcgggcg gcttggccgt ccaggtcagc gcctgctcca gcttcgcccc
      541 acgatagagc gcgccggggt cacggttcag actcagccaa tcgttgacgt cggccgtcaa
      601 ttgccgatgg cgaataagtc cctcacgatc ctgattcagc caatcgcgca gggtcggcca
      661 ttcgcggatg agcgcctcgt gggccacctc gacgaccttc gtctcgcccg ggggaatgac
      721 gtcggtcatt accagacgag agtcgatcag gatggacagc accgcgtcga gcgtcgccgc
      781 gtcggtcgaa cgggtgatga gttcgctgaa ctgggcgcgg cggcgcgtgt cgggcgtgtc
      841 gctgtcggcg ctttcgccca gttccgtcag acgcatgaag atcatgcggg caatcggctg
      901 ctgttccggc gtcagacgct ggcggaagac cgtctcggct gttttggcaa tagccccgcg
      961 cacgccgccc gactctttat aaccggatag cgtcatcgtg cggccgcgtc gtcgcttcca
     1021 tgtctccagc aaggcatgag agagtagcgg cagcgcgccc ggttcgtcgc ccacgtcgtc
     1081 gagcatcagc tcgaccagac cctcttgcag cttccacttg tcacgagcgg cgggcaggac
     1141 gatgacccga aacagctcct cgcggctcat cgccccgatg tattcctgat gccgggagac
     1201 aagttcgcgc aggccttcgt attgggcgca gtgggcgtag aaatcggcgc gaaggacaat
     1261 gacgatgagg ataggctggt cgccgccggc cgccgtcacc aagttgtcga tgaaggcgcg
     1321 gcgatcggcc tcatggcgaa caagcgagaa aagttcttca aactggtcga cgatgaggag
     1381 taacttcggt tgcgacttgc ccaaggcatc gggaagcgcg tccacgagcg ccgtcggccc
     1441 ggctcccagt cgatcgcgca gagcagccac ggccgccggc tctccatcgg gcaacagcgc
     1501 ggtcgccagc gcatcaagcg gattggccgt gggcgtcatc acccggatga gccagctgtc
     1561 cgaaccggtg ggcggcagcg cgccgtcggc caacggcttg ccacgccgca gcgcgggcac
     1621 caacccggcc cgcacaaccg acgatttgcc gctgccggac gcgccgatga cggccagaaa
     1681 gcgcgtctcc cgcagctgcc cgaccagctt ggctgtcagt gtctcgcgac cgagaaagtg
     1741 gtcggcgtcg gcctcatcga aatattgcaa tcccttatag gggggatctc ccgcttcggg
     1801 ggccaggccc tcaatgtcct gggccgcggc cgaattgacg aatgtcgact cgatgttgac
     1861 aatcgccccg cgaaaatcgc cggagaggtg ataggatttt ccgcctgctg ttgtgtcttc
     1921 gttatcaggg gcaggcatag gcttgtctca gcttt
//
LOCUS       DAOMQG010000527         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22573_length_1954_cov_8.374934, whole genome shotgun sequence.
ACCESSION   DAOMQG010000527 DAOMQG010000000
VERSION     DAOMQG010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22573_length_1954_cov_8.374934"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PLC06_16825"
     CDS             complement(<1..1448)
                     /locus_tag="PLC06_16825"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRO92167.1"
                     /translation="MEPQPVPTQYQKLLNHVHELNDLGKAAALLYWDRETYMPHGGTA
                     ERIAQMTTMSSLIHRMSTSDEAGELIENAAAEIDGAPYDSNEAGLIRLLRRTYADARK
                     LPAEYVARSSAISGQTREAWVLARKENDFAHFQPWLEQVIALCQEEAGYYGYEDERYD
                     ALLDKYETNMKTAEVRAIFDAVKAELVPLREAIDNSPVKLDTGIVHQPYDVEKQKAFA
                     RYIATALGYDFERGHLGTVVHPFAISFSRNDARITTRWYPDFLNPSLFGTLHESGHAM
                     YEQGTHPDLSRTPLARGTSLGIHESQSRMMENIVGRSRGFWQAHFPTLQGYFPEALGN
                     STAEDFYQAINKTQPSFIRVEADELTYNFHIILRFELEQAMLNGDLVAADLPAAWNDK
                     MRELLGITPPNDSQGCLQDVHWSRPGFGYFPTYALGNLYGAQFFETAVAQNPAITEEL
                     AQGKTTALLAWLRENIHQHGKKFTPGELVQRVT"
     gene            1646..1885
                     /locus_tag="PLC06_16830"
     CDS             1646..1885
                     /locus_tag="PLC06_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92168.1"
                     /translation="MVKTDRFTFKLDAEDRRRLGLLAQEVRRSQSDTLRWLIRMASSH
                     LADPVDVAAPEDMAAPEDYMAGDADRPEAWAGGGQ"
BASE COUNT          309 a          538 c          719 g          388 t
ORIGIN      
        1 gtgacgcgct gcaccagttc gccgggggtg aacttcttgc cgtgctggtg gatgttctcg
       61 cgcagccagg ccagcagcgc ggtggtcttg ccctgggcca gttcctcggt gatggccgga
      121 ttctgggcga cggccgtctc gaagaactgc gcgccataga gattccccaa agcataggtc
      181 gggaagtagc cgaagcccgg ccgcgaccag tggacgtcct gcaggcagcc ctggctgtcg
      241 ttcggcgggg tgatgccgag caactcccgc atcttgtcgt tccaggccgc cggcaggtcg
      301 gcggcgacga gatcgccgtt gagcatggcc tgctccagct cgaagcgcag gatgatgtgg
      361 aagttgtagg tcaactcgtc ggcctcgaca cggatgaacg atggctgggt cttgttgata
      421 gcctgataga aatcctcggc ggtgctgttg cccagcgcct cggggaaata gccctgcagc
      481 gtggggaagt gcgcctgcca gaagccccgg ctgcggccga cgatgttctc catcatccgc
      541 gactgtgact cgtggatgcc cagcgaggtg ccgcgcgcca gcggcgtgcg cgacaggtcg
      601 gggtgcgtgc cctgctcata catggcgtgg ccgctctcgt gcagcgtgcc gaacagcgag
      661 ggattgagaa agtcgggata ccagcgggtg gtgatgcgcg cgtcgttgcg gctgaagctg
      721 atggcgaagg ggtggacgac cgtgcccaga tggccgcgct cgaagtcgta gccgagggcg
      781 gtggcgatgt agcgggcaaa cgccttctgc ttctcgacgt cgtagggctg gtggacgatg
      841 ccggtgtcga gtttcaccgg gctgttgtcg atggcctcgc gcagggggac gagttcggcc
      901 ttgacggcgt cgaagatggc ccgcacctcg gccgtcttca tattcgtctc gtacttgtcc
      961 agcagcgcgt cgtagcgctc gtcctcatag ccgtagtagc cggcctcttc ctggcacagg
     1021 gcgatgacct gctccagcca cggctggaag tgggcgaaat cgttctcctt gcgcgcgaga
     1081 acccaggcct cgcgcgtctg gccgctgatg gccgacgagc gagcgacata ctccgccgga
     1141 agcttgcggg cgtcggcata ggtgcgacgc agcaggcgga tgaggccggc ttcattgctg
     1201 tcgtagggcg cgccgtcgat ctcggccgcg gcgttctcga tcagctcgcc cgcctcgtcc
     1261 gaggtggaca tgcgatggat gaggctgctc atggtggtca tctgcgcgat gcgttcggcc
     1321 gtgccgccgt ggggcatgta ggtctcgcga tcccagtaga ggagcgcggc cgccttgccg
     1381 aggtcgttca gttcgtggac atggtttagc agtttttggt attgggtggg gacgggttgg
     1441 ggttccatga gggggatttt atcgtgtgtg ggcggggggc acaagatgtg agtcaggtct
     1501 ggccctgggg gaaaggggga tggcgagcgc cctgtggaaa ccgcgttttt tcctgaaacc
     1561 ccggttgcct tgtttgaaaa tcgaatagca cgaatgttct attctttcat acaattgccg
     1621 tatcttttat gctggaggta cgacgatggt caagacagac agattcacgt ttaagctgga
     1681 cgcggaggac cggcggcggc tggggctgct ggcgcaggag gtgcggcgct cgcagagcga
     1741 cacgctgcgc tggctcatcc gcatggcgtc ttcgcatttg gccgatccgg tggatgtggc
     1801 ggcgccggag gacatggcgg cgccggagga ctatatggcc ggagacgccg accggccgga
     1861 ggcatgggcg ggaggcgggc aatgagaacc gaggaacgac ctgtctatga tgtctggtcg
     1921 cccaagtggc gggtgctggg ggtgacgctg tcgg
//
LOCUS       DAOMQG010000528         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22648_length_1947_cov_8.949260, whole genome shotgun sequence.
ACCESSION   DAOMQG010000528 DAOMQG010000000
VERSION     DAOMQG010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22648_length_1947_cov_8.949260"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="PLC06_16835"
     CDS             <1..1884
                     /locus_tag="PLC06_16835"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRO92169.1"
                     /translation="HWCHVMAHESFENPATAELMNRYYVNIKVDREERPDLDSIYMSA
                     VQALTGQGGWPMTVFLTPDGRPFYGGTYYPPVPRHGMPAFPQLLVALAEAWADKREEI
                     ERSAGEITTHLSQIAMAGGGDEGVLTTDLFDQALNGLLRTYDNKLGGFGHAPKFPPSM
                     TLEFLLRMHVQRGDAMALRMAEHTLEMMARGGIYDQLGGGFARYSTDDRWLVPHFEKM
                     LYDNALLARVYLHAWQLTGKPLYRRVVEETLDFVVRELRHEEGGFYSSYDADSEGEEG
                     KFYVWSAEEIHTALGSDAELFMAYYDVSNAGNWEGHNILNIRRDPAEVAAAFGLDATA
                     MEQTLTTARQTLYDIRARRVWPGLDDKVLTAWNGLMLAAFAEAGRALSRLDYTDIATQ
                     NAEFLYTNLRQTDGRLLRSWRAGTRARYNAYLEDYAFLADGLLALYQTTFEPRWFLWA
                     RELAEWMLTHFQDSAGGFFDTSDDHETLLHRPRDVQDNATPSGNSMAAHVLLALSLYT
                     GEGRYWDTAESMVAALAEPMMRYPGAFSHWLCAAALIMAEPREVAIVGDPAAADTREL
                     LDVLQSRYRPALVAAVGPSEPVVPLLAGRPQLNGRATAYVCRRFVCLNPVTQPEALAE
                     QLL"
BASE COUNT          384 a          613 c          599 g          351 t
ORIGIN      
        1 cattggtgcc atgtgatggc ccacgagtcc ttcgaaaacc cggctacggc cgaattgatg
       61 aataggtatt atgtcaatat aaaggttgat cgggaagaac ggcccgacct ggacagcatc
      121 tacatgagcg ccgtccaggc cctgaccgga cagggcggct ggccgatgac ggtttttctg
      181 acacccgacg gtcgcccctt ctatggcggc acctattatc cacccgtgcc gcgccacggc
      241 atgccagcct tcccgcaact gctggtggct ttagccgagg cctgggccga caagcgcgag
      301 gagatcgagc gcagcgcggg ggagatcacc acgcatctga gccagatagc aatggcagga
      361 ggcggcgacg aaggcgtgct gacgaccgac ctgttcgacc aggcgcttaa cggcctgctg
      421 cgcacctatg acaacaagct gggcggattt ggtcacgcac ccaagttccc gccgtcgatg
      481 acgctggagt tcctgctgcg catgcacgtc cagcggggcg acgcgatggc cctgcgcatg
      541 gccgaacaca cgctggagat gatggcccgc ggcggcatct acgaccagtt gggcggcggt
      601 tttgcccgct actcgactga cgatcgctgg ctggtgcccc atttcgagaa gatgctctac
      661 gataatgcgc tattggcccg cgtctatctc catgcctggc agctgaccgg caaaccgctc
      721 taccggcgcg tcgttgaaga gacactcgat ttcgtcgttc gtgagttacg gcatgaggaa
      781 ggcggattct acagcagcta cgacgccgac agcgagggcg aagaggggaa gttctacgtc
      841 tggtcggccg aggaaatcca caccgcactc ggatcagatg ccgaactatt tatggcctat
      901 tacgacgtca gcaatgccgg caattgggaa ggacacaaca tcctcaacat ccggcgcgat
      961 ccggccgagg tcgccgcagc atttggcctg gacgcaaccg ccatggagca gacgctaacg
     1021 acggcgcggc aaaccctcta cgacattcgt gcccggcgcg tgtggccggg actggatgac
     1081 aaggtgctga cggcctggaa cggcctgatg ctggccgctt ttgccgaggc agggcgggca
     1141 ttgagccgac tcgactacac cgacatcgcc acccaaaacg ccgagttcct ctacaccaac
     1201 ctgcgccaga cagacggccg cctgctgcgc tcctggcgtg ccggtacgcg agcgcgctac
     1261 aacgcctatc tggaggatta cgctttcctg gccgacggac tactggcgct ctatcaaacc
     1321 accttcgaac cgcgttggtt cctgtgggcg cgggagttgg ccgaatggat gctgacccac
     1381 ttccaggact cggcgggcgg cttcttcgac acgtccgacg accatgagac gctgctccat
     1441 cggcccaggg acgtgcagga caacgccacc cccagcggca acagcatggc cgctcacgtt
     1501 ctgctcgcgt tgagtctcta caccggcgag gggcgctatt gggatacggc cgaatcgatg
     1561 gtagcggcac tcgctgagcc gatgatgcgc tatcccggcg cgttttccca ttggctatgc
     1621 gcggcggcat tgatcatggc cgagccgcgc gaggtcgcca tcgtcggcga tccggccgcg
     1681 gccgacacgc gcgagttgct ggacgtcttg cagagtcgct accgaccggc actcgtcgcg
     1741 gccgtggggc caagcgagcc ggtcgtcccc ttgttggccg gccgcccgca actgaacggc
     1801 cgcgccactg cctatgtctg ccgccgcttc gtctgcctga acccggtcac gcaaccggag
     1861 gcattggcgg aacaactgct atagaataat atgttatgaa gagtctgttc cagaaattaa
     1921 ccaaatcagg caaggatgaa tttatca
//
LOCUS       DAOMQG010000529         1938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22770_length_1938_cov_8.896973, whole genome shotgun sequence.
ACCESSION   DAOMQG010000529 DAOMQG010000000
VERSION     DAOMQG010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22770_length_1938_cov_8.896973"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1938)
                     /gene="gcvP"
                     /locus_tag="PLC06_16840"
     CDS             complement(128..>1938)
                     /gene="gcvP"
                     /locus_tag="PLC06_16840"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRO92170.1"
                     /translation="RRIATRVHLLTEVMAAGLKQLGYEVNAHPVFDTLTIRGGRRGDI
                     ELRGAALAHEVNLRYNDDGSVGLSLDETVTVADLETLFAVFGAKEGEIDIDALADAAD
                     LDYAEPLRRASDFMTHPVFNSYLSEMEMMRYIKRLENRDISLVHSMIPLGSCTMKLNA
                     AAEMLSITLPGFAALHPFVPLDQAQGYQELLHNLESWLSEITGFAGCSLQPNSGAQGE
                     YTGMLTIRAYQIARGEGNRNVCLIPASAHGTNPASAAMAGMEVVVVASDDNGNVNVDD
                     LRAKAQQHRDDLSALMITYPSTHGVYEEAIGEICKIIHDNGGQVYMDGANMNALVAIC
                     RPGDFGPDVIHLNLHKTFAIPHGGGGPGMGPICVAEHLVAFLPNHPVVPGVGGTEALG
                     TVSAAPWGSASILPIPYAYIAMMGEWGLRVATEIAILNANYMAEKLAPHYPVLYRGLN
                     DRVAHECIVDPRGLKDTSGITAEDITKRLMDFGFHAPTLSFPVPGTLMIEPTESESLA
                     EIDRFIEAMVIIREEIRAIEEGRMDREDNPLKHAPHTAEVVLAETWDRPYTREQAAYP
                     ADWVRRAKVWPSVSRIDNVYGDRNLFCVCIPVEEYA"
BASE COUNT          346 a          582 c          642 g          368 t
ORIGIN      
        1 actggttttt aaggagagaa actttatcga aatgtggttg atggcgcaag aaatgcgtcg
       61 gaggttggtt tgtccaaaag agaaagacct ctgaggtttg ccgcaaacct cagaggtctt
      121 gtgtcatcta cgcatactcc tcaaccggaa tacacacaca gaacaaattc cgatccccat
      181 acacattatc gatccggctg accgacggcc agaccttggc gcggcgtacc cagtcggccg
      241 ggtaggcggc ttgctcgcgg gtgtaggggc gatcccaggt ctcggccagc acgacctcgg
      301 ccgtgtgcgg cgcgtgcttc agcggattgt cctcgcggtc catccgccct tcttcgatgg
      361 cgcgaatctc ctcgcggatg atgaccatcg cctcgatgaa acggtcgatt tcggccagcg
      421 attcgctctc tgtcggctcg atcatcagcg tgcccggcac cgggaatgac agggtcggcg
      481 catggaagcc gaagtccatc agccgcttgg tgatgtcttc ggccgtgatg cccgacgtgt
      541 ccttcaggcc gcgcggatcg acgatgcact catgggccac gcgatcgttc agcccacgat
      601 agagcaccgg gtaatgggga gccagcttct cggccatgta gttggcgttc aggatggcga
      661 tctccgtggc cacgcgcagc ccccattcgc ccatcatggc gatgtaggcg tagggaatgg
      721 gcaggatcga cgcgctgccc cacggcgcgg ccgagaccgt gcccaatgct tccgtgccgc
      781 ccacgccggg gacgaccggg tggttgggca ggaacgcaac caggtgctcg gcgacgcaga
      841 tcggccccat tcccggccca ccgccgccgt gggggatggc gaacgtcttg tgcaggttca
      901 gatggatgac gtccggcccg aaatcgccgg ggcggcagat ggcgaccagt gcgttcatgt
      961 tcgcgccgtc catatagacc tgcccgccgt tgtcgtggat gatcttgcag atctcgccga
     1021 tggcctcttc atagacaccg tgagtcgagg gataggtgat catcagcgcc gacaggtcgt
     1081 cgcggtgctg ctgagccttg gcccgcaggt cgtcgacatt gacgttgccg ttgtcgtcgg
     1141 aagcgacgac aaccacttcc atgccggcca tcgccgcgct ggcggggttg gtgccgtggg
     1201 cgctggcggg gatgaggcag acgttgcggt tgccctcgcc acgggcaatc tggtaagcgc
     1261 gaatggtgag catgccggtg tattcgccct gcgcgccgga gttgggctgc agggagcagc
     1321 cggcaaagcc ggtgatctcc gacaaccacg actccagatt gtggagcagt tcctgatacc
     1381 cctgagcctg atcgagcggc acgaacgggt gcagcgcggc gaagccgggc agggtgatag
     1441 agagcatctc ggccgcggcg ttgagtttca tcgtgcacga gccaagcggg atcatcgagt
     1501 gcaccagcga gatgtcgcgg ttttccaggc gcttgatgta gcgcatcatc tccatctccg
     1561 acaggtagct gttgaagacg ggatgggtca tgaaatcgct tgcgcgccgg agcggctcag
     1621 cataatccag atcggccgcg tcggccaggg cgtcgatgtc gatttcgccc tctttcgcgc
     1681 cgaacacggc gaagagggtt tccaggtcgg ccacggtgac ggtttcgtcg agcgacaggc
     1741 cgaccgagcc gtcgtcgttg tagcgcaggt tgacttcatg ggccaacgcc gcaccgcgca
     1801 gttctatgtc cccgcgacgg ccgccgcgga tcgtcagcgt gtcgaagacc ggatgcgcgt
     1861 tgacctcata gccgagctgc ttaagcccgg cggccatgac ctcggtcagc aggtgaacgc
     1921 gggtggcgat gcgccgta
//
LOCUS       DAOMQG010000530         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22805_length_1936_cov_8.571505, whole genome shotgun sequence.
ACCESSION   DAOMQG010000530 DAOMQG010000000
VERSION     DAOMQG010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22805_length_1936_cov_8.571505"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..>1936
                     /locus_tag="PLC06_16845"
     CDS             393..>1936
                     /locus_tag="PLC06_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HRO92171.1"
                     /translation="MAIPIPSMILKQLHTNGSLKNTADGVEFSMKNRLNDTKLVGLTG
                     LKFDGREVALDKVRVRLDDGTLLTATQLAAAPVDFPLRRTLDIVAAIEPLAVGKHEIE
                     IRFEAQPFGKLKLKVDDAITAQDGPKMAHIPRDNADDYGESAIKTRQKFVEEYTGAKL
                     EHTPQYSFDPHITAGNAEHFIGVAQIPLGIAGPMTVNGEYAQGDFLVPLATTEGTLIA
                     SYNRGMKVMNLSGGVTCTVIDDAMQRAPVFVFNNAREARHFVKWVEANIGEVRGQAEA
                     TSSVAKLQYIDYYLASKLAYLRFNFSTGDAAGQNMVGRATFAACSWILDHYEGIQHFY
                     LESNLATDKKASQVNVMHTRGKRVVAECTIKRDVLIQHMRVEPESLFYHSGIANVGSI
                     LSGANNNGAHSANGITAMFIATGQDVANVSESSAGIIYSEMTPEGDLYLSFTIPSLIV
                     ATHGGGTGLATQQECLKMLGCVGRGKVNKLAEIVAGVVLAGEISLAAAISSSDWVSSH
                     EKYGRN"
BASE COUNT          423 a          578 c          583 g          352 t
ORIGIN      
        1 ggcgcttcca caacgccgtc gagcgagatg aacaaaccgg atgtaacttt gcgcatgatg
       61 ggtctccttt gaaagctgga atgttgaaca aatgttcttg ttattttaat cgaaacagtt
      121 gcaattgcaa gaactgcaat tgtttgaagt aagcaacttt taccaaatag cgaattattt
      181 caggatagtt gtaggggcgg gtttcaaacc cgcccttacc taaatgaata aggggataga
      241 ccactcaaaa tagttgagca atcgtcccat tcccgctaca ctccccatcc aaatgacgag
      301 tgacgaatga cgagtgacga atttggaatc gcgctcttca ttcgtaattc gtaattccta
      361 attcgtaatt tctctctgga ggcaggtcga ctatggcaat tcccattccc agcatgattt
      421 tgaagcagtt gcacacgaac ggcagtctga agaacacggc cgacggcgtg gagttctcga
      481 tgaagaaccg gctcaacgac accaaactcg ttgggctgac cggtctcaag ttcgacggcc
      541 gcgaggtggc gctcgacaag gttcgcgtgc ggctcgacga cggcacgctg ctgaccgcga
      601 cccaactggc ggccgcgccg gtcgattttc cgctgcggcg cacgctcgac atcgtggccg
      661 ccatcgagcc gctggccgtg ggcaagcacg agatcgagat tcgcttcgag gcccagccgt
      721 tcggcaagct gaagctgaag gtcgacgacg ccatcaccgc gcaggacggc ccgaagatgg
      781 cccacatccc ccgcgacaac gccgacgact acggcgagag cgccatcaag acccgccaga
      841 agttcgtcga ggagtatacc ggcgcgaagc tggaacacac gccgcaatac tcgttcgacc
      901 cccacatcac cgccggcaac gccgagcatt tcatcggcgt ggcccagatt ccgctgggca
      961 tcgccgggcc gatgacggtt aacggcgaat atgcccaggg cgacttcctc gtgccgctgg
     1021 ccaccaccga gggcacgctc atcgccagct acaaccgcgg catgaaggtc atgaacctgt
     1081 cgggcggggt cacctgcacc gtcatcgacg acgccatgca gcgcgcgccg gtctttgtgt
     1141 tcaacaacgc ccgcgaagcg cgccatttcg tcaaatgggt ggaggccaac atcggcgaag
     1201 tccgcgggca ggccgaggcg acgtcgagcg tggccaagct gcagtacatc gactactacc
     1261 tggccagcaa actggcctat ctgcgcttca acttcagcac gggggacgcg gcggggcaga
     1321 acatggtcgg ccgcgccacc ttcgccgcct gctcgtggat cctcgaccat tacgagggca
     1381 tccagcattt ctatctggag agcaatctgg ccaccgacaa gaaggcgtca caggtgaacg
     1441 tgatgcacac gcgcggcaag cgggtggtgg cggagtgcac catcaagcgc gacgtgctga
     1501 tccagcatat gcgcgtggag ccggagagcc tgttctatca ttcgggcatc gccaacgtcg
     1561 gctccatcct gagcggggcc aacaacaacg gcgcgcactc ggccaacggc atcacggcca
     1621 tgttcatcgc cacggggcag gacgtggcca atgtgtcgga gtcgagcgcg gggattatct
     1681 attcggagat gacgccggaa ggtgatctgt atctgtcgtt caccatcccg tcgctcatcg
     1741 tggccaccca cggcggcggc acggggctgg ccacgcagca ggaatgcctg aagatgctgg
     1801 gctgcgtcgg ccgcggcaag gtcaacaagc tggccgagat cgtcgccggg gtggtgctgg
     1861 ccggggaaat ctcgctggcg gcggccatct cctcgtcgga ctgggttagc agtcatgaga
     1921 agtatgggcg gaacag
//
LOCUS       DAOMQG010000531         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_22963_length_1925_cov_10.171123, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000531 DAOMQG010000000
VERSION     DAOMQG010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22963_length_1925_cov_10.171123"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLC06_16850"
     CDS             complement(<1..388)
                     /locus_tag="PLC06_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92172.1"
                     /translation="MYPEDRVLVAYVPRPSDFDIIRREGWYRIPQRHAPKGLHAEYLA
                     FYFGSKFGEEKYAIHYYAQRAGDELARRRDLLPDEPNHPHADDIYFKVQLGPLQKLAQ
                     PIVSLRWRRVTFVHTTWDRFKDATEIN"
     gene            512..874
                     /locus_tag="PLC06_16855"
     CDS             512..874
                     /locus_tag="PLC06_16855"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HRO92173.1"
                     /translation="MSGINVSLELIKPLEQVAIERGESITSIVEKLIADFLRDQRHLF
                     LMKEMDRFRSLHPGLRAQYEGTYIAMRDGHVLDHDVDGNQLYARVRERLGDTPVLIVE
                     VTDQPEQLFTRTSQRIAS"
     gene            871..1281
                     /locus_tag="PLC06_16860"
     CDS             871..1281
                     /locus_tag="PLC06_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92174.1"
                     /translation="MNYPYNHQFRPPAPFLTVRLYSFVSDTFTPSIPAMVDTGSDASL
                     VPLPLLLQLGAEETAPGWLVSITGERKPVSLFFVDVHIDDQILAGIRVIGEPDATEVI
                     LGRDVLNHLSLFLDGPESTLVIPEEKTVTRLRQS"
     gene            1290..>1925
                     /gene="mutM"
                     /locus_tag="PLC06_16865"
     CDS             1290..>1925
                     /gene="mutM"
                     /locus_tag="PLC06_16865"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRO92175.1"
                     /translation="MPELPEVETFVRALRKPLVGRTITGVRNDWPRHIAIPSPDELRR
                     RITGRRIGAIDRRAKYLVFSLSDDETLIIHLKMSGHLSVVAAETPADKYAHTVFELDD
                     GRELRFRDPRKFGRVYLLRDPAYVLGSLGPEPLAEAFTVEALATRLAGRKRVLKPLLL
                     DQTFIAGIGNIYADEALFYAGIRPARSSDSLTAGETAALHAAIQKVLAMGIE"
BASE COUNT          388 a          579 c          544 g          414 t
ORIGIN      
        1 cgttgatctc cgtcgcatcc ttgaagcgat cccaggtggt gtgaacaaac gtgacccgtc
       61 gccagcgcag gctgacgatg ggctgggcca gcttttgcag cgggccaagc tgtaccttga
      121 agtagatatc gtcggcatgg gggtgattcg gctcgtcggg cagcaggtcg cggcggcgcg
      181 ccagttcatc ccctgcgcgc tgggcgtagt agtggatggc gtatttctct tcgccgaact
      241 tgctgccgaa gtagaacgcc aaatattcag cgtgcagacc cttgggtgcg tggcgctgcg
      301 ggatgcgata ccagccctcg cggcggatga tgtcgaaatc cgacgggcgc ggcacgtagg
      361 ctaccaggac gcgatcttcg gggtacatgg cctatagttt acagtgaaag ctccaaatgt
      421 cagccataga attccgttca cttgtcagta aattcgccct tcaagcgcgg atattttcta
      481 atcgatgcct tgatctggta taattgagtc gatgtcagga atcaacgttt ccctggagtt
      541 gatcaaaccg ctggagcagg tagcaattga gcgcggtgag tcgatcacgt ctatcgttga
      601 gaagctgatc gcggacttcc ttcgggatca gcgtcatctc tttctgatga aagagatgga
      661 tcggtttcgg tcgctccacc ccgggcttcg ggcacaatac gagggaacat acatcgcaat
      721 gcgcgatgga catgtgctgg atcatgacgt tgatgggaat caattgtatg ctcgcgtgcg
      781 cgaacgatta ggcgacacgc cggtgctgat tgtcgaagtc actgaccagc ccgaacaact
      841 ctttacgcga accagccaaa gaattgcctc gtgaactatc cttataatca tcagtttcgg
      901 ccgccggctc cttttctgac agttcgcctt tattcattcg tctccgacac attcacccca
      961 tcaatcccgg cgatggttga taccggttcg gatgcttcgc tagttcccct tcccttattg
     1021 ctccagctcg gcgctgaaga aacagctccc ggatggttgg tcagtattac aggagaacga
     1081 aagcccgtat ccctattctt tgtagacgtt cacatcgatg atcaaattct ggccgggatt
     1141 cgcgtgattg gcgaacctga cgcaaccgaa gtcatcctgg gaagagatgt attgaatcac
     1201 ttatccctct ttctcgatgg cccggaatcg actctcgtca taccggaaga aaagaccgtc
     1261 acccgacttc gccaaagtta gcccctctta tgcctgaact accggaagtc gaaacctttg
     1321 tccgcgccct gcgcaagccg ctggtcggcc gcaccatcac cggcgtgcgc aacgactggc
     1381 cgcgccatat cgccatcccg tcgccggacg agctgcgccg tcgcatcacc ggccgccgca
     1441 tcggagccat cgaccggcgc gccaagtacc tggtgttcag tctgagcgac gatgagacgc
     1501 tgatcatcca tctcaagatg agcggtcatc tgtccgtcgt agcggccgaa acgccggccg
     1561 acaaatacgc ccacaccgtc tttgaactcg acgacggccg cgagctgcgc tttcgcgacc
     1621 cgcgcaagtt cggccgcgtc tatctgctgc gcgatccggc ctacgtcctc ggctcgcttg
     1681 ggccggagcc gctggccgaa gcgttcaccg tcgaagcgct ggcgacccgc ctggccgggc
     1741 gcaagcgcgt gctgaagccg ctgctgctcg accagacctt catcgccggc atcggcaata
     1801 tctacgccga cgaagcgttg ttctatgcgg gcatccggcc ggcgcggagc agcgacagcc
     1861 tgacggccgg cgagacggcc gcactccacg ccgccatcca aaaagttctg gcgatgggca
     1921 tcgag
//
LOCUS       DAOMQG010000532         1919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23040_length_1919_cov_5.318670, whole genome shotgun sequence.
ACCESSION   DAOMQG010000532 DAOMQG010000000
VERSION     DAOMQG010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23040_length_1919_cov_5.318670"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1255
                     /locus_tag="PLC06_16870"
     CDS             26..1255
                     /locus_tag="PLC06_16870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRO92176.1"
                     /translation="MQENPPVPETLRSRVALGLEPWLIGHAPLMSLGHLILEASFNFL
                     PVTYVLLIPKLGLTYGQIGTLALLSSLAGTLAQPFFGWLSDHGAPRFIVVGSLVWGGV
                     LMGLVGFMPTYAALGVLLVLAALGSAAFHPPAAALSSLTEARNRGRAMSLFSVGGHLG
                     AAFSPALVGLALGMVGLASTAVVIPLSLVTAWFLARRLRGLTSVSNRGRAAQIDDAHR
                     ADASAVAALLAMVAVTGARSYFQLALMTYLPEWLRGAGQSLAMAGAGLAILMVAVSVG
                     SLFGGPLSDRFGRLRVIILSFVLTIVGYWLMLSLTGWPQMMAVILVGLMVGASFPPTI
                     VLAQEAWPGSVGFASAMVMGMGWLPAGLGAWVVGHIADESTLTDGLRTLLFVPLVSLA
                     AMGVYLLVRPRAKAQEG"
     gene            complement(1266..>1919)
                     /locus_tag="PLC06_16875"
     CDS             complement(1266..>1919)
                     /locus_tag="PLC06_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRO92177.1"
                     /translation="KSNAFIRPSPAGGVLGGVARKTRETMLVCEAAGFDVVIVETVGA
                     GQNEVTVRSMVDFFLVVLIPGGGDELQGIKKGIVELADAIAINKADGDNKPRAEAARA
                     EYNRALHYLTSPTEGWRPRAYTCSAQTGEGVDALWDVVLRFHERAAGTGALDARRREQ
                     SREWLHTMIDEQLRALFYDRPAVVAALPDIEAAVMDGTLPPATAARRLLALLESKQP"
BASE COUNT          228 a          648 c          689 g          354 t
ORIGIN      
        1 aaacaatgct atgatccgac cttctatgca agagaatccc cccgtccccg aaacccttcg
       61 atcgcgtgtg gccctcggcc tggagccgtg gctgattggg cacgcgccgc tcatgtcgct
      121 gggccacctc atcctggagg ccagcttcaa ctttttgccc gtcacctacg tgctgctgat
      181 ccccaaattg gggctgacct acggccagat cggcacgctg gcgctgctgt cctcgctggc
      241 cggcacgctg gcccagccgt ttttcggctg gctgagcgac cacggcgccc cgcgcttcat
      301 cgtcgtcggc agcctggtct ggggcggcgt gctcatgggg ctggtggggt tcatgcccac
      361 ctatgccgcg ctgggcgtgc tgctggtgct ggcggcgctc ggttcggccg cctttcatcc
      421 gcccgcggcg gcgctctcct cattgaccga ggcgcggaac cgcggccggg ccatgtcgct
      481 cttctcggtt ggcggtcatc tgggcgcggc gttcagcccg gcgctggtcg gtctggcgct
      541 ggggatggtc ggtctggcgt ccacggcggt ggtcatcccc ctgtcgctgg tgacggcctg
      601 gttcctggct cgccgcctgc ggggcctcac ttcggtttcc aaccgcggcc gcgcggcaca
      661 gatcgacgac gcgcacaggg ccgacgcgag cgccgtggcc gcgctgctgg ccatggtcgc
      721 cgtcaccggc gcgcgcagct atttccagct ggcgctgatg acttacttgc ccgaatggct
      781 gcgcggcgcg gggcagtcgc tggcgatggc cggcgcgggg ctggccatcc tgatggtcgc
      841 cgtcagtgtc ggcagcctct ttggcggccc gctgtcggat cgcttcggcc gcttgcgggt
      901 gatcatcctg agcttcgtgc tgacgattgt cggctactgg ctgatgttgt cgctgaccgg
      961 ctggccgcaa atgatggcgg tcatcctggt cggcctgatg gtcggggcgt cgttcccgcc
     1021 gacgatcgtg ctggcccagg aggcgtggcc gggcagcgtg ggcttcgcct cggcgatggt
     1081 gatgggcatg ggctggctac ccgccgggtt gggggcctgg gtggtcggcc atatcgccga
     1141 cgaaagcacg ctgaccgacg gcttgcggac gctgctgttt gtgccgctgg tcagtctggc
     1201 ggcgatgggc gtctacctgc tcgttcggcc gcgggccaaa gctcaggaag gctaggccgg
     1261 ccgaatcagg gctgcttcga ctcaagcagg gccaacaacc ggcgcgcggc cgtcgcgggc
     1321 ggcagcgtgc cgtccatgac cgccgcctcg atatcgggca gggcggcgac caccgccggc
     1381 cggtcgtaga acagggcgcg caactgctcg tcgatcatgg tgtggagcca ttcgcgcgac
     1441 tgctcgcggc ggcgggcatc cagcgcgccc gtcccggcgg cgcgctcgtg gaagcgcagc
     1501 accacgtccc acagcgcgtc gaccccttcg ccggtctggg cggaacaagt ataggcgcgc
     1561 ggccgccagc cttcggtcgg cgaggtcagg tagtggagcg cgcggttgta ttcggcgcgg
     1621 gcggcttcgg cgcggggctt gttgtcgccg tcggccttgt tgatggcgat ggcgtcggcc
     1681 agttccacga tccccttctt gatcccctgc agctcgtcgc cgccgccggg gatgagcacc
     1741 accaggaaga agtcgaccat cgagcggacg gtgacctcgt tctggcccgc gcccaccgtc
     1801 tcgacgatga ccacatcgaa cccggccgcc tcgcagacga gcatcgtctc gcgggtcttg
     1861 cgggccacgc cgccgagcac gccgcccgcg ggcgaggggc ggatgaaggc gttggattt
//
LOCUS       DAOMQG010000533         1918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23057_length_1918_cov_6.500805, whole genome shotgun sequence.
ACCESSION   DAOMQG010000533 DAOMQG010000000
VERSION     DAOMQG010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_1918_cov_6.500805"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLC06_16880"
     CDS             <1..1373
                     /locus_tag="PLC06_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92178.1"
                     /translation="VSELPYAWTSLWGRFGYGQIPLPSWIYVALFGIFLLSIAGYGFA
                     GVKGSKGGRGQQHPFSPAPPLILLALNVAIFFAVLFNYLLVSPAGPMGRFFFPALSSL
                     AILMFYGLSLLVSTADGRRPTYDKTAAVGRQRSAVGVLALAANLFMLAISLIALFGYL
                     APAYARPPGYAAEAALPNATDIRFDSFVALRGYAVDTDAARPGEPIGIELYWEVLATP
                     PGDYLLFVHLTDDETGALVAQRDTHPGLGNFPSSQWRPGDRFVEQMKMYLPETAYAPS
                     AGELTVGLYAPEGYRLGVTDNATGEGLGDAYPIGRVTINSQAAPDGEGLPNAGDYNFE
                     DRIRLLGYDYDRRVLGVGEPIGLTLYWEGLRPSPGDYNVELTVLDASGNVVFSQTDPI
                     NSADWQPGEIVVGSYTLPTGTATGDLRVRLVLLDAATNRRVNRVGDDGRWIDDELFLS
                     AVATGR"
     gene            1622..1870
                     /locus_tag="PLC06_16885"
     CDS             1622..1870
                     /locus_tag="PLC06_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92179.1"
                     /translation="MDYGMIGKIEKAKFYAEERDTRIVFQSLHVSILGDNDKTHQVKY
                     DNGLWHCDCNYFGSHQVCSHTMAMERVLGSMVEMGTLA"
BASE COUNT          363 a          579 c          583 g          393 t
ORIGIN      
        1 ccgtgtcgga gctgccctac gcctggacca gcctgtgggg ccgcttcggc tatggacaaa
       61 tcccgctgcc gtcgtggatc tatgtcgcgt tattcggcat attcttgctg tctatcgccg
      121 gttatggctt tgccggagtc aaggggagca aggggggaag ggggcagcag caccctttct
      181 cccctgctcc ccctctcata ctcctggcgc tcaacgtggc tatcttcttc gccgttctgt
      241 tcaactacct gctggtcagc cccgcggggc cgatggggcg cttcttcttt ccggcgctgt
      301 cgtcgctggc gatcttgatg ttttatgggt tgagcctgct ggtttcgacc gccgacggcc
      361 gacggccgac ttacgacaaa acagccgcgg tcggccgtca acggtcggcg gtcggcgtgc
      421 tcgccctggc ggccaacctc ttcatgctcg ccatctccct catcgcgctg ttcggctatc
      481 tggccccggc ctatgcgcgg ccgccgggat atgccgccga agcggcattg cccaacgcca
      541 ccgacatccg cttcgattcg ttcgtcgccc tgcgcggcta cgccgtggat accgacgcgg
      601 cgcggccggg cgaaccgatt ggcattgagt tgtattggga agtgctggcc acgccgccag
      661 gcgactacct gctgttcgtc catctgaccg acgacgagac gggggcgttg gtagcccagc
      721 gcgacaccca ccccggcctg ggcaacttcc ccagcagcca atggcggccg ggcgaccgtt
      781 tcgtcgaaca gatgaaaatg tatctgccgg agacggccta tgcgccgtcg gccggggaac
      841 tgaccgtcgg cctctacgcg cctgagggct accggctggg cgtcaccgac aacgcgacgg
      901 gcgaagggtt gggcgacgcc taccccatcg gccgcgtcac catcaactct caagccgcac
      961 cggatggtga aggattgccc aatgccgggg actacaactt tgaggatcgc atccgcctgc
     1021 tgggctacga ttatgaccgg cgcgtgctgg gcgtcggtga gccgatcggc ctgacgctct
     1081 actgggaagg gctgcggccg tcgcccggcg attacaacgt ggaactgact gtgctggacg
     1141 cgagcggcaa tgtcgtcttc agccaaaccg acccgatcaa ctcggccgat tggcagccgg
     1201 gcgagatagt ggttggctca tatacgctgc cgacagggac ggcgaccggc gatctgcggg
     1261 tgcgtctggt gctgctcgac gccgccacca acaggcgcgt caacagggtc ggcgacgacg
     1321 gccgctggat cgatgacgag ctattcctgt cggccgtggc gacaggtcga taagctcaaa
     1381 ccggaaatgg gcctgcaaaa agagtgagcc tcgattgccc cgcaattgcc accggatcgt
     1441 aggttaatcg ttgcctggct cgattgacac cctataaggg ttttgtatac taagtacatg
     1501 gtgctgaaaa taattacagc agctcacaat cgaacagttt cgtacacgca cgcctgaccg
     1561 cgtgcgtttt tgtttccggc agtagagggt tgttcaagta gcaatagagg agatacacga
     1621 catggattat ggtatgatcg gcaagattga aaaggcgaag ttctacgcgg aagagcgaga
     1681 cactcgcatt gtctttcaat cacttcatgt gagcatcctg ggcgacaacg acaaaactca
     1741 ccaggtgaag tacgataatg ggctatggca ctgcgactgc aactatttcg gctcacatca
     1801 ggtttgtagc cacaccatgg cgatggagcg cgtcctcggc agcatggtcg agatggggac
     1861 tctcgcttaa tcgccacatt ccgacaatct aatacaagat aaagtgccct gtttctgc
//
LOCUS       DAOMQG010000534         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23160_length_1911_cov_6.561961, whole genome shotgun sequence.
ACCESSION   DAOMQG010000534 DAOMQG010000000
VERSION     DAOMQG010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23160_length_1911_cov_6.561961"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLC06_16890"
     CDS             <1..858
                     /locus_tag="PLC06_16890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRO92180.1"
                     /translation="ARRLMPKRPADGHKGTFGTALIAAGSIRYWGAPALAARGAFRAG
                     VGLVTLAVPQRLRPALAGQFPEAIYPLVTDTDTLGVSTANLLLASLSDYSALLVGPGL
                     GDAATFVGALLDGLSGVTDRLPPPLVMDADGLNMLAAMPDWPQRLPPNTVLTPHPGEM
                     ARLVGRPLSAVRDEDRVELARGKAAEWGHVVVLKGAYTVIAAPDGRATLLPFANPVLA
                     VGGSGDVLAGIIVALLAQKMPPYEAAALGAYLHGAAGEMARAYWGDAGILAGELADWI
                     SHVHRALTG"
     gene            908..>1911
                     /gene="alr"
                     /locus_tag="PLC06_16895"
     CDS             908..>1911
                     /gene="alr"
                     /locus_tag="PLC06_16895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRO92181.1"
                     /translation="MINGSTITSSGVLRPTQVEVDLSRLTANYRAIQAHVAPAAVMPI
                     LKANAYGHGLVEVALHMQSLGAPYLGVAFLEEGILLREAGVTTPILVLGGIIGNQVPL
                     FLAHDLTLTASSIEKLGQIEEAARAMGVRAKVHLKIDTGMERIGVHYYSAASLLEASL
                     TGDHFEVEGIFSHFANADAADSSYSRLQLERFYEVLDFYERRGLPPPPLRHIANSAAI
                     AGYPAARLDMVRAGILLYGVYPSADVPRTVAVSPALSWKSRVVYFKVVQPEHPVSYGS
                     TWQSDHPVRVVTVPVGYGDGYFRSMSGRAEVLIRNKRYPVVGRVCMDQLMVNIEWETA
                     "
BASE COUNT          290 a          603 c          643 g          375 t
ORIGIN      
        1 gctcgccggt tgatgccgaa gcgtcccgcc gacggccaca agggcacctt cggcacggcg
       61 ctcatcgccg cgggcagcat tcgctattgg ggtgcgccgg cgctggccgc gcgtggggcc
      121 tttcgcgccg gggtggggct ggtgacgctg gccgtgccac agcggttgcg tccggcactg
      181 gccgggcagt tccccgaggc gatctacccg ctggtgaccg ataccgatac actgggcgta
      241 tcgacggcca atttgctgct ggcctcgctg agtgactata gcgccttgct ggtcgggccg
      301 ggactgggtg atgcggcgac gtttgtcggc gcgttgctgg acggattgag cggcgtgact
      361 gataggctgc ctccaccgct ggtcatggac gccgacgggc tgaatatgct tgcggcaatg
      421 cccgattggc cgcagcggct gccgcccaat accgtgctga cgccccaccc gggggagatg
      481 gcgcggcttg tcggccgccc gctcagcgca gttcgcgacg aggatcgggt tgagttggcc
      541 cgcggcaagg cggccgaatg ggggcacgtc gtcgtcctga aaggcgcata cacagtgatt
      601 gccgcgccgg acgggcgggc gacgttgctg cccttcgcca atccggttct ggccgtcggc
      661 gggagtggtg acgtgctggc cgggatcatc gtagccctgt tggcccaaaa gatgccgccc
      721 tatgaggcgg ccgcgttggg ggcgtatctc catggtgccg cgggcgagat ggcgcgcgcc
      781 tattgggggg atgccggcat actggccggg gaactggccg actggatttc tcacgttcat
      841 cgcgccctga ccggctgagc cgtcgttaaa acaggcttat atccggttgc gatgatttaa
      901 cccgcctatg atcaacggct caaccatcac ctcaagcggc gttctgcgtc ccacgcaggt
      961 cgaagtcgat ctttcccgcc tgacggccaa ctatcgcgcc attcaggccc acgtcgcgcc
     1021 cgcggccgtc atgcccattc tgaaggccaa cgcctatggc catgggttgg tcgaggtggc
     1081 cctccacatg caatcgctcg gcgcgcccta tcttggcgtg gcctttttgg aagagggtat
     1141 cctgttgcgc gaggcagggg tgacgacgcc catccttgtg cttggcggca ttatcggtaa
     1201 tcaggtgccg ttgtttctgg cgcatgacct gacgctgacc gcttcatcca ttgaaaagct
     1261 gggccaaatc gaggaggcgg cacgggcgat gggtgtgcgg gccaaggtgc acctgaagat
     1321 cgacaccggc atggagcgta tcggcgtcca ctattacagc gccgcctcgc tgctggaggc
     1381 ctcactgacc ggggatcact ttgaagtcga gggcatcttc tcccacttcg ccaacgccga
     1441 cgcggcagac tcatcttact cccgactgca actcgaacgc ttttacgaag tcctcgactt
     1501 ctatgagagg cgcggcctgc ccccgccgcc gctgcgccat atcgccaact cggcggccat
     1561 cgccgggtac ccggccgccc ggctggatat ggtgcgggcg ggcatcctgc tctatggcgt
     1621 ctatccgtcg gccgacgtgc cgcgcacggt ggccgtctct ccggccctga gctggaagtc
     1681 ccgcgttgtc tacttcaagg ttgtccagcc ggagcatccg gtcagctatg gttcgacctg
     1741 gcagagtgac catccggtgc gcgtggtcac tgtgccggtt ggttacggcg acggctattt
     1801 ccgcagcatg agcggccggg ccgaagtgct tatccgcaac aagcgttacc ctgtggtcgg
     1861 ccgcgtgtgc atggatcagc tcatggtcaa catcgagtgg gagacggcct a
//
LOCUS       DAOMQG010000535         1899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23333_length_1899_cov_7.934924, whole genome shotgun sequence.
ACCESSION   DAOMQG010000535 DAOMQG010000000
VERSION     DAOMQG010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23333_length_1899_cov_7.934924"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1899
                     /locus_tag="PLC06_16900"
     CDS             <1..>1899
                     /locus_tag="PLC06_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRO92182.1"
                     /translation="LLFSQLFPLTIPWSNSLLRDHPATSIAALLSAAPYLLAGAALNT
                     PAAIIVGLFAGVGRLIGQTGQPLDVVAMALAAGLSAMLMQQNFAGRGFKLLRLPIIAG
                     TLGRLVVVIFTGAAIFFASYRLAGLFGALDLALYLGAWSALPLLIEGAVGGAITSAVQ
                     WIAPQWRLDRGTVPSPFQRSLQRQLVATFLTFAAAVVLLSVLVAFFFSARATERALTR
                     QMVDNADATSAQLGALQSRLTNTLAEYIRDPELAEEDPGRKSAVLGRVKETRLFDNVR
                     LVSPEGVHSSGADATLDPSELKLATSALDAGQARWIAIASENGSTAIDLVVPGASTGG
                     EPIVLYARVATEALDEIASELVKVGGQSQGMVINELSQVVIASNDRASTEWLAPSNEQ
                     IASQLSNITGRTVYESLNPVTGARELIGISPVPESGWKVAAFLPRSVILRQTLGIIGP
                     LTVLLLIVSGLFYALVAGLGRDITQPIAEIGRASKAIAGGGGLERPVRSHREDEIGQL
                     TLAFSQMQRALRQRLDELSLLLSVSNEVAATINIYEGMSAVLQGVLRGTGASGARAIV
                     RNPAAPAPLIFAEGPGADAMSELDRIILLRVRETEELAFSTSAEIEEALAIHPSPVAA
                     LFALPLRP"
BASE COUNT          357 a          578 c          572 g          392 t
ORIGIN      
        1 cgctcctctt tagccagtta tttccgctga ctattccatg gtcaaactcc ctgctgaggg
       61 atcacccggc gacgagcatc gccgcgctcc tctcggccgc gccctacttg ctggccgggg
      121 cagccctcaa tacgccggcg gcgatcatcg tcggtctgtt tgcaggcgtt ggccgcctca
      181 tcggccaaac cgggcagccg ctggacgtcg tagcgatggc tctggcggcc ggattgtcgg
      241 caatgctgat gcaacaaaac ttcgccgggc gcggctttaa gttgctgcgc ctacccataa
      301 tcgcaggaac gttgggccgg ttggtcgttg taatatttac cggagccgcc atatttttcg
      361 cttcctatcg tttggcaggt ctgttcggcg cgctggacct ggcgctttac ctcggggcct
      421 ggagtgcgtt gccactcctg atcgaaggcg cagttggcgg cgcaatcacc tcggccgtcc
      481 agtggattgc accacagtgg cgtttggatc gcgggaccgt cccctctccg ttccagcgca
      541 gcctgcaacg acaattggtg gctacattcc tgacgttcgc tgctgcagtc gttctgctca
      601 gcgtgctggt cgccttcttc ttttcagccc gggcgactga gcgagccttg acccgacaga
      661 tggtcgacaa cgcggatgca acgtccgctc aacttggtgc gctgcagagc cggctaacca
      721 acacgttggc tgagtatatt cgcgatcctg aattggccga agaagaccca ggccgaaaat
      781 cggccgtgtt gggacgtgtg aaggagactc gcctgttcga caacgtgcga ctcgtcagtc
      841 cggaaggggt acactcatcc ggggccgacg ccacccttga cccctccgaa ttgaagctgg
      901 ccacctctgc actcgatgcc ggccaagcgc gctggattgc gattgcgagc gaaaacggga
      961 gcacggccat tgatctggtc gtgccgggag cgtcgaccgg cggagaaccg attgtactct
     1021 acgcgcgtgt cgccacagaa gcgctcgatg agatcgcgag cgagttagtg aaagtcggtg
     1081 ggcaaagcca agggatggta atcaacgaac tgtcacaggt ggtcatagcc tcaaatgaca
     1141 gagcaagcac ggaatggctt gcccccagca acgagcagat cgcctcacag ctctccaata
     1201 tcaccggccg cacagtgtat gagagcttaa accctgtcac cggcgcgcgc gaactaatcg
     1261 ggatttcacc agtgcccgaa agtggctgga aggtcgcggc gtttttgccc cggtcggtga
     1321 ttttgcgcca gacgttgggc attattgggc cgctgaccgt actgttactc attgtgagcg
     1381 gcctgtttta tgcacttgtg gccggccttg gccgcgacat tacccaacct atcgcggaaa
     1441 tcggccgtgc ctcgaaagcc attgccggag ggggcggtct tgaacgtcct gtccgctccc
     1501 accgtgaaga cgagattggg caactgacgt tggcattctc ccaaatgcaa cgcgccttgc
     1561 gacaacgact cgatgaatta tcattattgc ttagcgtcag caacgaggta gcggcaacca
     1621 tcaacatcta cgagggcatg tcggccgtgt tgcaaggtgt gctgcgcggc accggtgctt
     1681 cgggagcacg agccatcgtc cgcaacccag cggcgcctgc cccgctgatc ttcgccgaag
     1741 gccccggcgc cgacgccatg agcgaactgg atcggatcat tttgctgcgc gtgcgcgaga
     1801 cggaagaact ggcctttagc acatcggcgg aaatcgagga agcactggcg atccatccat
     1861 ccccggtagc ggcgctattc gcgctgccgt tgcggcccg
//
LOCUS       DAOMQG010000536         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23457_length_1890_cov_11.223978, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000536 DAOMQG010000000
VERSION     DAOMQG010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23457_length_1890_cov_11.223978"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLC06_16905"
     CDS             <1..239
                     /locus_tag="PLC06_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HRO92183.1"
                     /translation="LRWLDKHEREPEEASPLGIILCTGKKREQIELLELDKSGIHVAE
                     YLTSLPPRAVLGEKLQQATERARLQIEQRQHDTE"
     assembly_gap    455..547
                     /estimated_length=93
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            573..1082
                     /gene="radC"
                     /locus_tag="PLC06_16910"
     CDS             573..1082
                     /gene="radC"
                     /locus_tag="PLC06_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRO92184.1"
                     /translation="MSQLSFSSFDSSLMVRDAQGRYLLATAEQILEAARQAIERKMQR
                     GTSFTSPAAVKEYLRAKLAGFEHEVFAVLFMDTQHRLIEYAEMFRGTIDGASVYPREL
                     VKEALRLNAAAVIVSHNHPSGNPEPSGADRALTQRLKEALGLVDVRVLDHVIVAGTDT
                     TSFAERGLI"
     gene            1360..1710
                     /locus_tag="PLC06_16915"
     CDS             1360..1710
                     /locus_tag="PLC06_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HRO92185.1"
                     /translation="MNNALITDEQRIVLLANGRESLESPDFDPAPVVKLFTPDAGATW
                     LLTEIDPDDHDHAFGLCDLGLGEPELGWVSLQELATVRGRFGLPVERDLHFRAEKRLS
                     AYARDARLAGRVVV"
BASE COUNT          291 a          579 c          603 g          324 t
ORIGIN      
        1 acttgcgctg gctggacaag cacgagcggg agccggaaga agcctcgccg ctggggatca
       61 ttctctgcac cggcaagaag cgcgagcaga tcgagttgct ggagctggac aagtcgggca
      121 tccacgttgc cgagtacctg accagcttgc cgccgcgtgc ggtgctgggt gagaagttgc
      181 agcaggcgac cgagcgggcg cggttgcaga tcgagcagcg ccagcacgac accgagtagg
      241 ccaagcagca ttcgtgcgtc ccggcgtgcc gccgtcgggt tcgcgggctg cgccccgcgc
      301 ttcgtgccga atcgcggcca tccggctttg ccccctgacg ccttcggccc tggcgggcct
      361 gcgcgctccg cttgcacaaa gacgagtgtg tgcaatgggc gggtgtaggc ggtcttgctg
      421 ttcccttcac cgtatcacgg cgttctcgcc gtcannnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnccg cccgagcaac cggagcacga tcatgtcgca actgtccttt tcctcgttcg
      601 actcctcgct gatggtgcgt gacgcgcagg gccgctacct gctggcgact gccgagcaaa
      661 ttctggaggc cgcgcgccag gccatcgagc gcaagatgca gcgcggtacg tcgttcacgt
      721 cgccggcggc ggtcaaggag tacctgcgtg ccaagctggc cggcttcgag catgaggtgt
      781 tcgcggtgct gttcatggac acgcagcatc ggctgatcga gtacgccgag atgttccgag
      841 gcaccatcga cggcgcatcg gtgtatccgc gcgagctggt caaggaggcg ctgcggctca
      901 acgcggcggc ggtcatcgtt tcgcacaacc acccgagcgg caatcccgag ccgagcgggg
      961 ccgaccgagc actgacccag cggctcaagg aggcgctggg tctggtggac gtgcgcgtgc
     1021 tggatcacgt catcgtcgcg ggcaccgaca ccacatcgtt tgccgaacgt ggcctgatct
     1081 gacgaagggg cttcggcccc tttttgctgc gcccggcgcg ggccgggcga aaacgctgcc
     1141 tcggatttcc gagacagcgt ttcggaaatt catggcctgc atggaggtat ggggcggtct
     1201 tgctgtcccc ttcatcgtgc cacggcgttc tcgccgtcaa gggctgcgcg ccgatggcgc
     1261 ttgcgtcctg gcggccgtcg ttgacccctg actgcttgcg ctgcgccgtg ccccggaagg
     1321 gtcgggcaat tccgcccggc aacctttcag gagttcacca tgaacaacgc actcatcacc
     1381 gacgagcagc gcatcgtgct gctggccaac ggccgcgaat ccttggagag cccggacttc
     1441 gatccggcgc ccgtggtcaa gctgttcacg ccggatgccg gcgcgacctg gctgctgacc
     1501 gagattgatc ccgatgacca cgatcacgcc ttcggcttgt gcgacctggg cctgggagaa
     1561 ccagaactcg gttgggtcag cttgcaggag ctggcgaccg tgcgcgggcg attcggcctg
     1621 ccggtcgaac gcgacttgca ctttcgtgcg gagaaacgct tgagcgccta cgcgcgcgat
     1681 gcgcgactgg ctgggcgtgt cgttgtctga cctggcccct tggagcgccg caaggcgctc
     1741 catgtgcttt ctcgaccatt acaggagatc aatgccatga gcagccatca cgattgggtc
     1801 atcgaagttt cggcgcagca cgacgcacac aagccattcg cgccggagaa cggccagccg
     1861 ctgcgcttca agatcggcga cgcggtgatc
//
LOCUS       DAOMQG010000537         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23486_length_1888_cov_5.584834, whole genome shotgun sequence.
ACCESSION   DAOMQG010000537 DAOMQG010000000
VERSION     DAOMQG010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23486_length_1888_cov_5.584834"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1572
                     /locus_tag="PLC06_16920"
     CDS             139..1572
                     /locus_tag="PLC06_16920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRO92186.1"
                     /translation="MESRNNLNENNRRGMGLFMALIAAVMMMVLAGCGRASGPGAQSA
                     PAAAGETAAGQATATAPAVPFLGEVAATPIPPAIVTAAPAATATSAASPTPAASPTAD
                     GGVVATVAAAPVEVDRSATPAPFGNIKQPPYAVSDCSDKYPCNDDRAGWESRIQLPPG
                     FEATYYARIDGQPSVLTFGPDGLLYVGTMAGEIFTVDEAGESTLYVDGFNAPAGLAFQ
                     PGTARLYVASRLIDQNVDGEAWVGVVEDGVVTKLIDGLPCCYVAMHSANGIAFGADGF
                     AYMAVGGRADHGEILGGPNHGQQDELHPFEASILRFSPDGTQIDTYARGFRNSYDIAW
                     DADGVLFTTDNMPDFGPPEEFNRVVPGGEHGYPWYDCDVCFPAPPDVEIVPPLHTFIP
                     HTAPTGITAYLDDEFPGFYNSLFVTLWSAFPDGQRIVWFGPGGEETATFATGFAAPID
                     LTVGPDGALYVADWATGIIFRIAYVGE"
     gene            complement(1649..>1888)
                     /locus_tag="PLC06_16925"
     CDS             complement(1649..>1888)
                     /locus_tag="PLC06_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRO92187.1"
                     /translation="MLELALVRLMEIIGEAAGRISPETRAKLPEIPWSDVVAMRNRLI
                     HGYDNVDHDILWDTITHDLPLLLEAVDNLIEQASN"
BASE COUNT          337 a          592 c          624 g          335 t
ORIGIN      
        1 ggcgcatatg gatgtggaga ttcataatga cgggccggtg acgatgatga ttgagaagtg
       61 agaaggggga aagggagagg gggagaaagg gagaagggga gaaaggggtg gggcggcctt
      121 gttggagtat attgagatat ggaatcaaga aataatctga atgaaaataa tcgccgcggg
      181 atggggctgt ttatggctct gatcgcggct gtgatgatga tggtattggc cggttgcggc
      241 agggcgagcg ggccgggggc gcaatccgcg ccggcggccg cgggtgagac ggccgccggg
      301 caggcgacgg cgaccgcgcc ggccgtgccc tttctcggtg aggtggcggc gacgcccatc
      361 ccgccggcca ttgtgacggc cgcgcccgcc gcgacggcca cgtctgccgc ctcgccaacc
      421 cccgctgcct cgccgacggc cgatggcggt gtcgtggcaa ccgtggcggc cgcgccggtt
      481 gaggtcgacc gcagcgccac ccccgcgccg ttcggcaata tcaagcagcc gccctatgcc
      541 gtcagcgatt gcagcgacaa gtacccgtgc aacgatgacc gcgcgggctg ggaatcgcgc
      601 atccagctgc cgccggggtt cgaggcgacc tactacgccc gcatcgacgg ccagcccagc
      661 gtgctgacct tcggccccga cggcctgctc tacgtgggca cgatggccgg cgagattttc
      721 accgtcgacg aggccggcga gtcgacgctc tacgtcgacg gcttcaacgc gccggccggt
      781 ctggccttcc agccgggcac cgcgcggctc tacgtcgcca gccgcctgat tgaccagaac
      841 gtcgacggcg aggcctgggt gggcgtggtg gaggatggcg tggtgaccaa actcatcgac
      901 ggcctgccgt gctgctatgt ggccatgcac tcggccaacg gcatcgcttt cggcgccgac
      961 ggctttgcct atatggctgt tggcggccgc gccgatcatg gcgaaatcct gggcggcccc
     1021 aaccacggcc agcaggacga gctgcacccg ttcgaggcca gcatcttgcg cttcagcccc
     1081 gacggcacgc agatcgatac ctacgcccgc ggctttcgca actcctacga catcgcctgg
     1141 gacgccgacg gtgtgctctt cacgaccgac aacatgcccg acttcggccc gccggaagag
     1201 ttcaaccgcg tcgtgcccgg cggcgagcac ggctacccgt ggtatgactg cgacgtgtgc
     1261 ttcccggccc cgcccgacgt ggagatcgtc ccgccgctcc acaccttcat cccccacacc
     1321 gcgccgacgg gcatcaccgc ctacctcgac gatgagttcc ccggcttcta caacagcctg
     1381 tttgtgacgc tgtggagcgc gttccccgac ggccagcgga tcgtctggtt cggccccggc
     1441 ggcgaggaga cggcgacatt cgccaccggc tttgccgcgc cgattgacct gacggtcggc
     1501 cccgacggcg cgctgtatgt ggccgactgg gcgacgggga tcatttttcg gattgcgtat
     1561 gtaggggagt gatgagttat gagagacgag cgacgagatc ggtaggcggg ggtccttgtt
     1621 tcattgtgac cccatcctcg gatagaacct aattggaggc ttgttcaatc agattgtcaa
     1681 ccgcttccag caataatggc agatcatgag tgatcgtgtc ccataatatg tcgtgatcga
     1741 cattatcata cccatgaatg aggcgattac gcatggccac cacgtcagac cagggaattt
     1801 caggcagttt tgcccgtgtt tccggcgata tacgcccggc ggcttcccca atgatctcca
     1861 tcagacgaac gagcgccaat tcaagcat
//
LOCUS       DAOMQG010000538         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23621_length_1877_cov_7.671240, whole genome shotgun sequence.
ACCESSION   DAOMQG010000538 DAOMQG010000000
VERSION     DAOMQG010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23621_length_1877_cov_7.671240"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..683
                     /locus_tag="PLC06_16930"
     CDS             150..683
                     /locus_tag="PLC06_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_256874273.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:366..368,aa:Sec)
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRO92188.1"
                     /translation="MAFIDYVPFEQVPEKWQVKDRDNIIQIHSVHPQTLRHHYDLYLE
                     LMRKRGPLSRIQREMIAVVVSAENHCHYULHHHGAGLRRLLEGEGVAEAAREQLLADL
                     SADYRAADLDMADRAMLDYAMKLTREPWAVAAGDIIALREAGFDDRAIHDICAVTAYF
                     AFVNRIADGLGVELEGD"
     gene            complement(706..1227)
                     /locus_tag="PLC06_16935"
     CDS             complement(706..1227)
                     /locus_tag="PLC06_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRO92189.1"
                     /translation="MSSPVVDERLFQQLLQADKLGIRMEIVGGLPLWEASPVLRHQEA
                     LDRIRASIRPQTNQLDDDLKCDCFHAADVYVVFPDGSLKRPDIAIYCRRPDEDDEAIT
                     LIPEAVIEIISKGYEAKDLEIGPRFYLSQGVKDVIVFDPHTLVVLHLTPTSAARHVSP
                     IDITLACGCACRV"
     gene            complement(1359..>1877)
                     /locus_tag="PLC06_16940"
     CDS             complement(1359..>1877)
                     /locus_tag="PLC06_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRO92190.1"
                     /translation="YAEYSVPTRGILGFRQPFLTATRGTGVFHALFDAYLPYAGDIAH
                     TEFGSLVSLEGGPVSGYALEHLTARGTFFVRPSIDEVYPGQVVGEHIRDEDLIINVCR
                     TKNLTGHRAKPTAIVEALSPPRLMTLDEAIEYLHEDELLEVTPESLRIRKMVLDHNMR
                     NREAKRVKYAET"
BASE COUNT          359 a          530 c          621 g          367 t
ORIGIN      
        1 cccgggttgc tgccttgatt catacaaggg agcaacccgg gtgaatgtag tgcggcgaaa
       61 aatgtcctgt gttaagcgac gagcggcgag cgcgtttcag gctcgtcact cgtcgtttgt
      121 cactcgtcac tcgtataagg aaatcaaaca tggcctttat cgattacgta ccgttcgaac
      181 aggtacctga aaaatggcag gtcaaggacc gcgacaacat tatccagatt cacagcgtcc
      241 acccccagac gctgcgtcat cattatgacc tgtatctcga gcttatgcgc aaacgcgggc
      301 cgttgagccg catccagcgc gagatgatcg ccgtcgtcgt ctcggccgag aatcactgtc
      361 actattgact gcaccatcac ggagcgggtc tccgtcgatt gctggagggt gagggcgtgg
      421 cggaggcggc gcgggagcaa ctcctggccg acctcagcgc cgattaccgc gcggccgatc
      481 tggatatggc cgatcgggcg atgctcgatt acgccatgaa gctcactcgc gagccgtggg
      541 ccgtcgctgc cggagatatc atcgcattgc gcgaggcggg cttcgacgac cgggccattc
      601 acgacatctg cgccgtcacg gcttattttg ctttcgtcaa tcgtatcgct gacgggttgg
      661 gggtggagct ggagggcgat tgacgcgccg ggtggcgcat tccggctaga cccggcaggc
      721 gcagccgcag gccagggtga tgtcaatggg cgagacatgg cgcgcggccg aagtgggggt
      781 caggtggagg accacgaggg tgtggggatc aaagacgatc acgtccttca ccccctgcga
      841 caggtagaag cgcgggccga tctccaggtc cttggcctcg tagcccttgc tgatgatctc
      901 gatgaccgct tcggggatca gggtgatggc ttcgtcatct tcgtccggcc ggcgacagta
      961 gatggcaatg tcgggacgtt tgagtgagcc atcggggaag accacataaa cgtcagcggc
     1021 gtggaagcaa tcgcatttca gatcgtcgtc caactgattg gtttgcggac ggatggacgc
     1081 gcgaattcga tccaatgctt cctggtggcg caagacgggg ctggcctccc aaagaggtag
     1141 gccgccgaca atttccattc gtatgcccag tttgtcggcc tgaagcaact gctgaaataa
     1201 acgttcgtcg acgacgggcg aggacatggc ggcattatac catggatccg gcagcgaatg
     1261 taatcgggtt gcaggcccac cagggtaatc gcgtatgaat tgcctcatac gtgcgggttc
     1321 aaacccgcag cggacaggca aaaccggtgc gataacgact acgtttccgc gtacttcacc
     1381 cgcttcgcct cgcggttgcg catgttgtgg tcgagcacca tcttgcggat gcgcagcgac
     1441 tccggcgtca cttcgagcag ttcgtcttcg tgcagatatt cgatggcctc gtcgagcgtc
     1501 atgaggcgcg gcggggagag ggcctcgacg atggccgtcg gcttggcgcg gtggccggtc
     1561 aggttcttgg tgcggcagac gttgatgatc aggtcttcgt cgcggatgtg ctcgcccacg
     1621 acctgcccgg gatagacctc gtcgattgac ggccgcacga agaacgtgcc gcgcgccgtc
     1681 agatgctcca gcgcatagcc gctgaccggg ccgccttcca gcgacaccag cgagccgaac
     1741 tcggtgtggg cgatgtcccc ggcatagggc aggtaggcgt cgaacagggc atggaacacg
     1801 cccgtgccgc gggtggcggt caggaacggc tggcgaaagc cgaggatgcc gcgggtgggc
     1861 acgctgtatt cggcgta
//
LOCUS       DAOMQG010000539         1870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_23735_length_1870_cov_9.123416, whole genome shotgun sequence.
ACCESSION   DAOMQG010000539 DAOMQG010000000
VERSION     DAOMQG010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23735_length_1870_cov_9.123416"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PLC06_16945"
     CDS             <1..1799
                     /locus_tag="PLC06_16945"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRO92191.1"
                     /translation="FQVAEAISRHADREGMIAEVARLVPILTGVSLCGFLLWDQDSES
                     YFGASLVNADGDEDAYFGDVELDIGEWGALDAIHVGGHPLTTAHLPLWLRRHESSCVR
                     LFPLCSTQGKNLGAMFVGLTADDACDDDPTSRQANTSNERLREELVQNVATQLERALE
                     EAYLRLAQQEEAWVNTALLQVAEAVNSLTDLNEILDTIVRLVPLLVGVDSVFILVWDE
                     ARQIFQAGPSYGVSTMGRGLVETLEIDRDEFLAMSPQLAPDFDQPRPPSATYYALRVP
                     SWLEAVLNTSNAYSFPLVARGRLVGAKIVGLQRERPGRALFSTRRINILNGIAQQAAT
                     AVVNNQLYKESADRARMQQELDVAHTIQASFLPDGSPKIPGCSVATHWQAARQVGGDF
                     YDFLPLGDDKWGIVIADVADKGVPAALFMAVSRTILRTVAFSHLDPAYVLTRTNEIIA
                     REARSDLFVTVFYGVWDPSTERFTYANAGHNPPLLLQPDGAFQPLPGHGIALGVVPKA
                     HMESFSTQLQPGETIIFYTDGVTDALNEDFGEFGLERLQIAARAAARRPAADLVETIT
                     DNIRDHTGQTPQFDDMTLIVLKRHAIKPRGQK"
BASE COUNT          360 a          612 c          554 g          344 t
ORIGIN      
        1 aattccaggt ggcggaagcc atcagccgcc acgccgatcg cgaagggatg atcgccgaag
       61 ttgcccggct ggtacctatc ctgaccggcg tgtccttgtg cggcttcctg ttatgggatc
      121 aggacagcga gagctacttc ggagcgtcac tggtcaatgc cgacggagat gaggacgcct
      181 attttggcga cgttgagctt gacatcggcg aatggggcgc gctggatgcg atccatgtcg
      241 gcggtcaccc actcaccaca gcccacctcc cgttatggct gcgccggcac gaatcgtcct
      301 gtgtgcgcct gttccctctt tgcagcacgc agggcaaaaa cctgggcgcc atgttcgtcg
      361 gtctgacggc cgatgacgcc tgcgatgacg acccgacgag ccgccaggct aacacgtcca
      421 atgaacggct gcgggaagag ctggtccaga acgtagccac ccaattggag cgggcgctgg
      481 aagaagccta cctgcgcctg gctcagcagg aagaagcctg ggttaacacc gccctgttac
      541 aggtggccga ggcggtcaat agcctgacgg atttgaatga aatcctggat acgatcgtgc
      601 gtctggtgcc cttgctcgtg ggcgtcgatt cggtgtttat tctggtctgg gatgaagcgc
      661 ggcaaatctt ccaggccggg ccaagctacg gcgtaagcac catggggcgc ggtctggtgg
      721 agaccttgga aatcgatcgg gatgagttcc tggccatgtc gccacagttg gcccccgact
      781 tcgatcagcc gcggccgccg agcgccacct actacgcctt gcgcgtgcca agctggctgg
      841 aagcggtcct taacacgtcg aacgcctaca gttttcccct ggtggcccgc ggccgtctgg
      901 tgggcgcgaa gatcgttggg ctgcaacgcg aacgcccggg ccgcgcgcta ttctccaccc
      961 gacgaatcaa catcctgaac ggtatcgccc agcaggcggc cacagcggtc gtcaacaatc
     1021 agctctacaa ggaatcggcc gatcgggcgc gcatgcaaca ggagctggac gtcgcccaca
     1081 ccatccaggc cagcttcctg cccgacggca gccctaagat acccggctgc tccgtcgcca
     1141 ctcattggca agcggcccgg caagtcggcg gcgacttcta cgactttctg ccgctgggcg
     1201 acgacaaatg gggtatcgtc atcgccgacg tggccgacaa gggcgtgccg gcagcgctct
     1261 tcatggccgt aagccgcacc atcctgcgca ccgtggcctt tagccatctg gacccggcct
     1321 acgtcctcac gcgcaccaac gagattatcg cccgcgaggc ccgctccgat ctgttcgtga
     1381 ccgttttcta tggcgtatgg gatccatcca ccgagcgctt cacctatgcc aacgccggcc
     1441 ataacccgcc attgcttctg cagcccgacg gggctttcca gccattaccc ggccacggca
     1501 tcgcccttgg cgtcgtgccg aaagcccata tggagagctt ttccacgcag ttacaaccgg
     1561 gagagacaat catcttctat accgacggcg tcaccgatgc cctgaacgag gatttcggcg
     1621 agttcgggct ggagaggttg cagatcgccg cccgcgccgc ggcccgccgt ccggcggccg
     1681 atcttgtcga gaccattacc gataacatcc gcgatcacac cggacaaacg ccccagttcg
     1741 acgatatgac cctgattgtc ctcaaacgac acgccataaa gccgagaggg cagaaataga
     1801 caagacgccc ccttcgggaa accatcgccc ggcgcgtgtc atctggctgc tcatcatcgc
     1861 gatcgctctg
//
LOCUS       DAOMQG010000540         1854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24011_length_1854_cov_9.758199, whole genome shotgun sequence.
ACCESSION   DAOMQG010000540 DAOMQG010000000
VERSION     DAOMQG010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24011_length_1854_cov_9.758199"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PLC06_16950"
     CDS             complement(<1..1634)
                     /locus_tag="PLC06_16950"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRO92192.1"
                     /translation="MDSQTQIDQTRYRRILQFFSSLIAHVIWWDLVVRHMPVAGQRAL
                     AGRSQRHRQMAREFRVLAIEMGGVMIKLGQFLSARVDMLPIEVTEELKGLQDEVPPAD
                     SEEVMGLLREQLGDISRRFEYIEPEPLAAASLGQVYRARLRPPRGQSGPGEQVVIKIQ
                     RPQIDFMVRTDLAALRVVARWLMRYKPIRKRANVPALMEEFARTLWEELDYNSEADNA
                     EHFARIHADDPHIYIPKVYREHSTQRVVVLEYVDALKIGDNEGLTKAGIKREEVAEML
                     LEAYFTQVFIAEFFHADPHPGNLFVRPLSPALDPVTGIGPTGAPASRQFQLVFVDFGM
                     AVRLPKAMGENLRKILVGVTQRDARQLTEAYRDLGFFLPGADIDRIAEAQEVILNQIW
                     GRKLLDLTRPDPREVEQIGREFRDLLFEMPFQIPQDFIYLGRAFGMLMGLVSQLDPQI
                     NPWRQVERYGQQLVRSQTLTHLRDLGLAGLVETIRPYLDTPFRIQRLLEEAEKGRLRV
                     QLKTDRDALHQQERLEKRVGQLGWSIVSAAGILSATL"
BASE COUNT          394 a          570 c          531 g          359 t
ORIGIN      
        1 agcgtcgccg acaggatacc cgcggccgaa acgatgctcc agcccaactg gccgacgcgc
       61 ttctccagcc gctcttgctg atgcagggcg tcgcgatccg ttttgagctg cacccgcaat
      121 cgccccttct ccgcttcttc cagcagccgc tgaatacgaa acggcgtgtc caggtatggg
      181 cgaatggttt ccaccagacc ggccaggccg agatcgcgca ggtgggtcaa tgtctggctg
      241 cgaaccagtt gctgcccata gcgttcgact tgtcgccagg ggttgatttg cggatccagt
      301 tgcgatacaa gccccataag catgccgaaa gcccggccca gatagatgaa atcctgcgga
      361 atctggaaag gcatctcgaa gagcagatcg cggaattcgc ggccgatttg ctcaacttcg
      421 cgcggatcgg gccgggtgag atcgagaagc ttgcgccccc aaatctgatt gaggatcact
      481 tcctgcgcct cagcaattcg gtcgatatcc gccccgggca ggaagaagcc caggtcgcga
      541 taggcctcgg tcaattggcg cgcgtcgcgc tgtgtaaccc ccacgagtat tttccgcaga
      601 ttctcgccca tggccttcgg caggcgaaca gccatgccga aatcaacgaa caccaattga
      661 aactgacgag aagccggcgc gccggttggc ccaatgccgg tgacgggatc aagagcaggc
      721 gacaacggcc gcacaaacag attgccggga tgtgggtcgg cgtggaagaa ctcggcgatg
      781 aacacttgcg tgaaatacgc ttccaggagc atctcagcga cttcctcgcg cttgatgccc
      841 gccttcgtca ggccttcatt gtcgccaatc ttaagggcgt cgacatattc gagaaccaca
      901 acgcgttggg tcgagtgctc ccgatagact ttagggatgt agatatgcgg gtcgtcggca
      961 tggatacgag cgaaatgctc ggcattatcg gcttcggagt tgtagtcaag ctcttcccat
     1021 aaggtgcgcg cgaactcttc catcagcgcc ggcacgttgg cccgcttgcg aatcggcttg
     1081 tagcgcatca gccaacgagc cacaacgcgc agtgccgcca gatcggtgcg caccatgaag
     1141 tcaatctgcg gtcgctggat cttgatgacg acctgctcgc ccggccccga ctggccacga
     1201 ggcggccgca accgcgctcg atagacctgc cccaacgacg ccgcagccaa cggttcaggc
     1261 tcgatgtatt cgaagcgtcg ggagatatcg cccaattgct cgcgcagcag gcccataacc
     1321 tcctcggagt cagccggtgg cacctcatcc tgcaacccct taagctcctc ggtcacttca
     1381 atgggcagca tatccacacg ggcgctgagg aactgaccga gcttgatcat caccccgccc
     1441 atctcgatgg ccagaacccg aaattcacgg gccatctggc ggtgtcgttg cgaccggccg
     1501 gccagcgccc gctgtcccgc aacgggcata tgacggacga caaggtccca ccagatcacg
     1561 tgggcgatca ggctggagaa gaattggaga attcgccggt agcgagtttg atcgatttgg
     1621 gtttggctgt ccatggtttg tcaggcgtgc cccgcagaga ccgttcgatc ccactcatag
     1681 tacgggggtg tgatccgttg atcaccgaat cattgtacca gattcagcga gaacaacctg
     1741 catcctcagg caacatttca tgataactcc aaataaaaat ccgcacgctt ttcatgcgtg
     1801 cggatcatgc cccatagggg actcgaaccc ctgtttcggc cttgagaggg ccgc
//
LOCUS       DAOMQG010000541         1853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24031_length_1853_cov_11.017798, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000541 DAOMQG010000000
VERSION     DAOMQG010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24031_length_1853_cov_11.017798"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1853
                     /locus_tag="PLC06_16955"
     CDS             <1..>1853
                     /locus_tag="PLC06_16955"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRO92193.1"
                     /translation="GQATPGSTAFTFLLILSAWWCYTYALELMTTDPTTLLFWVRLEW
                     ISIALLPVSWLLFALAYAGHARWVNLPVVMLLAAIPVVMIALAWTTPSHTWLYVNPGV
                     RDVGGIVVFSAERGPAYIIHVVYSYLLVLTATGLIIRVWFRSTGPQRQLASIVAVGAI
                     LPILSNAIYQFGIFGGLPIYVDLTVPAFAFSAVLFAWSWFRLHLFDLVPELIAPVPAF
                     DRLDAALATQSTQTRSLNLASLSLSILLFLTLSPIFTLLLREPATAGGRIAAYILIFL
                     FLLAIALWRDGSYLLRTIGLALVYLGLALLDLQISGLSPIVGFFLVTFAAFAAVLLPL
                     RLATPVVVIGIIIVGLVAMNVPAEVRPLPGGIYALSYLLLSLAMTAGMVVLILVVTRR
                     DIRTLLRLARRLSRDLEVERAQLEERVAERTRALETSAAVSRRLSTILDQRQLVHEVV
                     ELLRDAFSYYHVHIFLWDEALGALRMVGGTGEAGQAMLIMGHSLTPDVGLVGRAFSTN
                     SPVVVPDVTQDPGWLPNRLLPDTRTELAVPITYGDEVMGVLDAQDSEVNGLGPDDSQL
                     LQTIAGQLAVALRNARLLTQIQQEAEQAALINAINRKIAQTTDIEGAMRVAA"
BASE COUNT          286 a          595 c          544 g          428 t
ORIGIN      
        1 ggccaggcca cgccgggcag cactgccttt acttttttgc tcatcctctc ggcctggtgg
       61 tgttacacct acgcgctgga actgatgacc accgacccga cgacgctcct cttctgggtc
      121 aggctggagt ggattagcat cgccctgctg cccgtgtcct ggctgctctt cgctttggct
      181 tatgcggggc acgcgcgctg ggtcaacctg ccggtcgtca tgctactcgc ggcaattccg
      241 gtcgtcatga tcgcgttggc gtggacgacc ccatctcaca cctggctcta tgtgaatccc
      301 ggcgtccgcg atgtaggcgg tatcgtcgtc ttctcggccg aacgcggccc ggcttatatc
      361 atccatgtcg tctattccta tctgctcgtc ctgaccgcca cagggttgat cattcgcgtc
      421 tggttccgct cgacggggcc gcagcgacag ttggcttcca tcgtcgccgt gggcgcaatc
      481 ttgcccatcc tcagcaatgc catctatcaa tttggcattt tcggtgggtt acccatctac
      541 gttgacctga ccgtgcccgc gtttgccttc agcgcggtgt tgttcgcctg gagctggttc
      601 cggcttcatc tgttcgatct ggtgccggag ctgatcgcgc cggtcccggc gttcgaccgt
      661 ctcgatgcag cgctggccac gcaaagcacg caaacccgca gcctgaacct ggcttcatta
      721 agcctgtcga tactcctgtt tctgacgctg tcgccgattt ttaccttgct gctgcgtgag
      781 cctgccacgg ccggcggccg cattgcggcc tatatcctga tcttcctgtt tcttctcgcc
      841 attgcactct ggcgcgacgg ctcatatctt ctccgaacca tagggttggc attggtctac
      901 ctggggctgg cgctgctcga tctgcaaatc agcggcttgt cgcccatcgt tggcttcttt
      961 ctggtcacgt tcgccgcctt cgcggccgtg ctgctgccct tgcgcctggc gactccggta
     1021 gttgtcatcg gcattatcat cgtcggtctt gttgccatga acgtcccggc cgaagtccga
     1081 ccccttccgg gtggcatcta cgcgctcagc tatctgctgc tcagcctggc catgaccgcc
     1141 gggatggtgg ttctgatcct ggtcgtcacg cgacgtgata tccgaacgtt gttgcgcctg
     1201 gcccgaaggc tctcccgcga tctggaagtc gaacgggcgc aattggaaga gcgtgtggcc
     1261 gagcgcaccc gcgccctgga gaccagcgcc gcagtcagcc gccgcctctc gaccatcctc
     1321 gatcaacgac aactggtgca cgaagtggtg gaattattgc gcgacgcctt ttcctattac
     1381 cacgtccata tatttctgtg ggatgaagcg ttgggcgcgt tgcgcatggt cggcggcaca
     1441 ggcgaggccg ggcaggcgat gctgatcatg ggtcactcgc tgacgccgga tgtaggtctg
     1501 gtggggcgag ctttctcaac caatagcccg gtcgtcgtgc cggatgtgac gcaggacccg
     1561 ggctggctgc cgaatcggct attacccgac acacgaactg agctggccgt gcccatcacc
     1621 tatggtgacg aagtgatggg cgtactcgac gcgcaggaca gcgaagtgaa tggccttggc
     1681 cccgatgatt cccaactgtt gcagactatc gccgggcaac tggccgtggc cctgcgcaat
     1741 gcgcgcttgc tgacccaaat ccagcaagag gcggagcaag cggcgctcat caatgccatc
     1801 aaccgcaaga tcgcccagac gaccgacatc gaaggcgcga tgcgcgtggc cgc
//
LOCUS       DAOMQG010000542         1847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24135_length_1847_cov_7.801897, whole genome shotgun sequence.
ACCESSION   DAOMQG010000542 DAOMQG010000000
VERSION     DAOMQG010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24135_length_1847_cov_7.801897"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1514
                     /gene="fahA"
                     /locus_tag="PLC06_16960"
     CDS             252..1514
                     /gene="fahA"
                     /locus_tag="PLC06_16960"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRO92194.1"
                     /translation="MHLTIPIDSPFSLYTLPYSIFRPAVGEPARAGVAIGDWVLDLAA
                     LEADGVFDGPALRGQSIFAQPALNAFMALGRPAWDEVRARLLDLLAPVARNAPVAPEV
                     AHALRPRVSVELLLPANIGDYTDFYSSREHATNVGAMFRGPEQALMPNWLHLPIAYHG
                     RAGTIVAGDTMLGRPHGQMLPSGATTPVFGPTAELDFELEVGFFIGSGSRMGEPVTID
                     KAADHIFGLVLVNDWSARDIQRWEYRPLGPFLGKNFATSISPWVVPLAALEPFRCSGP
                     HQDPAPLSHLRGAENWAFDIQLQATLQSAAMRAAGLPPLVISRTNFRRLYWSMPQQLA
                     HHTSNGCVLRPGDLLASGTISGSTPDSYGSLLELTWGGERPLVLPTGETRSFLEDGDR
                     LALSGWCERDDYRVGFGEVAAEILPARD"
     gene            1516..>1847
                     /locus_tag="PLC06_16965"
     CDS             1516..>1847
                     /locus_tag="PLC06_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92195.1"
                     /translation="MAGQNDATKAQHRRVLNRAAGALFILAATAVALIAFGAFDPRPF
                     GPVVSTEQPGLQTVTGSGESVTPLAAPWPAFPDRLSIRLTAAHTTGEPDSGYGLTLGD
                     NDGRLLVAL"
BASE COUNT          311 a          615 c          552 g          369 t
ORIGIN      
        1 tctctgttgc acttgccgtc gagtcacccc gcccggctgt tagccggcac cttgccctgt
       61 ggagtgcgga ctttcctcag cttcggcgaa ccgaaaccgc gatcacccag ccagcttgcc
      121 acttataatc gtaccgggaa atcgagtcag gggcaataac tctcaacttg tacgggatcg
      181 ctatctaccg gttgccgcta ttcgagaacc gcattataaa aatgcaagat gattcaacac
      241 gaggcacaaa cttgcatcta accatcccga tagattctcc tttttcgctt tataccttac
      301 cctacagcat atttcgccct gccgtgggcg aaccggctcg cgcaggtgta gccattggcg
      361 actgggtgct cgatctcgcc gcgttggaag ccgacggcgt cttcgacggg ccggcactgc
      421 gcggacagag tatctttgcc caaccagcct tgaatgcttt catggcgctc ggccgcccgg
      481 catgggacga agttcgtgcc cggctgcttg atctgctggc ccccgttgcg aggaacgccc
      541 ctgttgcgcc ggaggtggcc cacgctttac gaccaagggt atcggttgag ctgctgctgc
      601 ccgccaacat cggcgactac accgatttct actcttcgcg agaacatgcc accaacgtcg
      661 gggccatgtt ccgcggaccg gagcaggcgc tgatgcccaa ttggctgcac ctcccgattg
      721 cctatcacgg ccgcgccggc acgattgtcg ccggggatac gatgctggga cgcccccatg
      781 gccagatgtt gccgtccggg gcgaccactc ctgtcttcgg cccaacggcc gaactggact
      841 tcgagctgga agtcgggttc ttcatcggct ccggcagtcg aatgggcgaa ccggtgacca
      901 ttgacaaagc ggccgaccac atctttggcc ttgtgctggt taatgactgg agcgcgcgcg
      961 acatccaacg ttgggaatat cggccgctcg gccccttcct gggcaaaaat ttcgccacct
     1021 cgatttctcc ctgggtcgtg ccgctggccg cgctggagcc gttccgctgt tccggcccac
     1081 atcaggaccc tgcccccctg tcccatctgc gcggggcgga gaattgggcg ttcgacattc
     1141 agctccaggc gaccctgcag agcgcggcca tgcgcgccgc cgggctgccg ccgctggtca
     1201 tctcgcgcac gaactttcgc cggctctact ggagcatgcc ccagcaattg gcccaccata
     1261 cgtctaacgg ctgcgtcctg cgtccgggcg acttgctggc ctccggcacc atcagcggct
     1321 ccacgcctga ttcgtatggc tcattgctgg agttgacctg ggggggcgag cgtccgctgg
     1381 tgctgccgac gggagagaca cgcagtttcc tggaggacgg cgatcgcctg gccctatcgg
     1441 gctggtgcga gcgagacgac tatcgcgtgg gcttcggcga ggtcgcggcg gagattctcc
     1501 cggccaggga ttgagatggc cggacaaaat gacgcgacga aggcacaaca tcgtcgggtg
     1561 ctaaacaggg cggccggcgc tctgttcatc ctggctgcca ctgccgttgc tcttattgcc
     1621 ttcggcgcgt ttgatccccg accatttggc ccagttgtgt cgaccgaaca acccggtctg
     1681 caaacggtga ccggctcagg tgaatccgtc acaccattgg ccgcgccgtg gcccgcattc
     1741 ccggaccgtc tctccatccg cctgacggcc gcccacacaa ccggggagcc ggacagcggc
     1801 tacgggctga cgctcggcga caacgacggc cggctgctcg tggcgct
//
LOCUS       DAOMQG010000543         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24280_length_1837_cov_7.213244, whole genome shotgun sequence.
ACCESSION   DAOMQG010000543 DAOMQG010000000
VERSION     DAOMQG010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24280_length_1837_cov_7.213244"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLC06_16970"
     CDS             <1..541
                     /locus_tag="PLC06_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRO92196.1"
                     /translation="VGGAIIMNDPDAHERLKFLQKAVGAVSGPMDCFLTLRGIKTLGI
                     RMRAHCDNGLAVARFLEDHPAVEQAIYPGLESHPQHELARRQMTGPGGMVSFLVRGGA
                     AAARTVALRTRLFALAESLGGVESLIELPSAMTHASVAGPEAAAAGFAVDPGLVRLSV
                     GIEHPDDLIADLKQALAGV"
     gene            583..1518
                     /locus_tag="PLC06_16975"
     CDS             583..1518
                     /locus_tag="PLC06_16975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRO92197.1"
                     /translation="MANGLSSVVHKMPYLKSIFLLFKDAFLAFRNDQASIYAAGLAYY
                     MVFSVAPLLVFVISIAGFFIDRTLAEEQVTSQLQYLMGGESADYIDGIVTTLRNQTLS
                     QTVTIVSIVALLISGAGIFGQLRTALNLIWGITNVRPANLREWLLLARYRAIPFLGVF
                     LLGTALALAVIAETALSTVEVRFEALFPDAYLLLPQLGRLIIPGLTFVAFLLVFVVLP
                     DAQIRWRDAAVGAAVTAVLFLIGRIVLAIFLALGNTGSIYGAAGSLIILLLWIYYSAQ
                     LLLYGAEFTRLYALRHGQAIRPNRLSRMMAAERDQ"
     gene            1693..>1837
                     /locus_tag="PLC06_16980"
     CDS             1693..>1837
                     /locus_tag="PLC06_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HRO92198.1"
                     /translation="MRIVVDAMGSDERPGPDVAGAVMAARKFGDTIILAGDKPLVEAE
                     LAAH"
BASE COUNT          308 a          553 c          573 g          403 t
ORIGIN      
        1 cgtcggcggg gcgatcatca tgaacgaccc cgacgcccat gagcggctca agttcttgca
       61 gaaggcggtc ggcgcggtat cggggccgat ggactgcttc ctgaccctgc gcggcatcaa
      121 gacgctgggc attcgcatgc gcgcccattg cgacaatggg ctggccgtgg cccgtttcct
      181 ggaagaccac cccgccgtcg agcaggccat ctaccctgga ctggagagcc acccgcagca
      241 cgagctggcc cgccggcaga tgaccgggcc gggcggcatg gtgtcgtttc tggtgcgtgg
      301 cggcgcggcg gccgcccgga cggtggcgct gcgcacgcgg ctgtttgcgc tggccgaatc
      361 gctgggcggc gtcgagtcgc tcattgagct gccctcggcc atgacccatg cctcggtggc
      421 cgggccggaa gcggccgccg ccgggttcgc cgtcgatccc gggctggtgc gtctctccgt
      481 cggcatcgag catcccgacg acctgatcgc cgatctgaag caagcactgg cgggggtgtg
      541 aatcaggaat caggaattac gaattaggaa tcacggaaga tagtggccaa cggtttgtcg
      601 tctgtcgtcc ataaaatgcc ctatctgaaa tcaatcttcc tgttattcaa ggatgcattc
      661 ctggcgtttc ggaatgatca ggcgtcgatc tatgccgcgg ggctggccta ttacatggtc
      721 ttttcggttg cgccgctgct ggtgtttgtg atcagcatcg ccggcttttt cattgaccgc
      781 acattggccg aagagcaggt cacatcccaa ctacaatacc tgatgggcgg cgaatcggcc
      841 gactatatcg acgggatcgt cacaacgctg cgcaatcaaa ccctgagtca gaccgtgacg
      901 atcgtgagca ttgttgcctt gctcatttcc ggggccggca tttttggaca attgcgaact
      961 gccctcaacc tcatttgggg catcaccaac gtgcgaccgg cgaatctccg cgaatggttg
     1021 ctgctggctc gctaccgggc tatccccttc ctcggagtgt tcctgctggg cacggctctg
     1081 gccctggctg tcattgccga gaccgcgctg tcgactgttg aggtccgctt cgaggcgttg
     1141 tttcccgatg cgtaccttct cctgccccag ttgggtcgcc tgatcatccc cggcctgacg
     1201 tttgtcgcgt tcctgcttgt cttcgtggtc ttgcccgatg cacagattcg ttggcgcgac
     1261 gcggccgtcg gcgcagcggt aactgcggtg ttgttcctca tcggccggat cgttctggcg
     1321 atcttcctgg ctcttggcaa caccggatcg atctacggcg cggccggatc gctcatcatc
     1381 ctgcttttgt ggatttatta ctcggctcaa cttctgctct atggcgcgga gttcacccgg
     1441 ctctatgccc tgcgccatgg tcaggccatc cgacccaatc gtctgtcgcg gatgatggcc
     1501 gccgagcgag atcaatgatc ggcgactatt ggccgacaac tgcggaccgg cgaccattga
     1561 ccgacgactg ctgcaagaaa tccatgcaga atgcagagaa ccggatgacg ggtggtagcg
     1621 cgcccctttt tctgcagccg ggccgattgt gatagaattc ccgctattat cctgcaaaaa
     1681 gagtagacaa gtatgcgtat agtagtcgat gcgatgggca gtgatgagcg acccggcccc
     1741 gatgtggccg gagcggtgat ggccgcgcgc aagtttggcg atacgatcat cctggcgggt
     1801 gacaagccgc tggtcgaggc ggagttggcc gcccatg
//
LOCUS       DAOMQG010000544         1829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24402_length_1829_cov_10.888388, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000544 DAOMQG010000000
VERSION     DAOMQG010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24402_length_1829_cov_10.888388"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..944
                     /locus_tag="PLC06_16985"
     CDS             24..944
                     /locus_tag="PLC06_16985"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRO92199.1"
                     /translation="MLAAEEINAPSIVARAHNLLGLIDRSRAKTQDAAAHFEEALALY
                     REIGNLQGQAQAQNSLANVYFDMGQWTDADRFYRQAGQTFSQLGNVYNRVLVDNNLGG
                     IALNQGRLDEALHFYQRALDALLQIGGSLWVTGALHLNLGATHIRRHELELAFAHLHD
                     SQELFEQAKVRDLLPEMHRRLAEAYLAQEDFGNAREEATRSLAIAEELAVPGEQGLTW
                     AVIGVIAATEGQLDEAETCLEKAVNLLREVGDDYGLACAQLSLAELYDGQDQTGSRND
                     LLAACKPTFERLGATIEIARAEILTDASAA"
     gene            complement(1027..>1829)
                     /locus_tag="PLC06_16990"
     CDS             complement(1027..>1829)
                     /locus_tag="PLC06_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92200.1"
                     /translation="CTFTNAPESTPIDDTIYVSPSRKGSVAGITYDDSDILRYTPAGQ
                     WQMYFDGSDVGLGLAGVKAFIMLSDNSILLSPTKALIIGGIGRIGPEDIIRFVPTGTG
                     ATTSGSFQMYFDGSDVDLTSRAEAIDAMSIDASGKLIISVRGRATVGVRPSGIIKARK
                     SDLLSFTFDTTGNSTSGTWAFHFDGADAGLNKENIDGLWIDPINNHHYLSVTNSFNLG
                     GGILGGGGTIFVCDPVSLGYSTSCNYSLYWDAAAAGLSPYIDGIHIQR"
BASE COUNT          405 a          461 c          531 g          432 t
ORIGIN      
        1 cgggccagca agccctggcg agcatgctcg cggccgagga gatcaatgcg ccgtcaatcg
       61 ttgcgcgtgc ccacaatttg ctgggcctga ttgaccgcag tcgggcaaaa acgcaggatg
      121 ccgcagccca ttttgaagaa gcgttggcgc tctatcgtga aatcggcaat cttcagggcc
      181 aggcacaggc acagaactcg ctcgccaatg tctactttga tatgggccaa tggactgatg
      241 ccgatcgatt ctatcgtcag gcaggtcaga catttagtca gttgggcaac gtgtataacc
      301 gggtactggt cgataataat ctggggggaa ttgccctgaa ccagggacgg ctggatgagg
      361 cgctgcattt ctatcaacgc gcgctcgatg cccttttgca gatcggcggt tcactctggg
      421 ttacgggtgc tttgcatttg aacctgggcg cgacacacat tcggaggcat gaactggagt
      481 tggcgtttgc ccatctacac gacagccagg agttatttga gcaagccaag gtgcgtgatt
      541 tgttgccgga gatgcatcgg cgattggctg aggcttatct ggcacaggag gatttcggta
      601 atgcaagaga agaggcgacg cgctcgctcg ccattgccga agagctggct gtaccgggcg
      661 aacaaggctt gacctgggcc gtgataggag tgatcgctgc gaccgaaggt caacttgatg
      721 aggcggaaac atgtctggag aaagccgtta accttttgcg tgaggttggg gacgattatg
      781 ggttggcatg cgcccaattg tccctggctg aactatacga tgggcaagat caaaccggct
      841 cacgaaacga tttgttggca gcctgtaaac ctacttttga gcgattgggt gcgacaatcg
      901 aaatagcgcg tgccgagata ttaacggacg cttcggccgc ttgacggtgc aattgccaac
      961 cataaaaaag aggggcttgt ttgcccctct tttcgttagt tgtcgcaggt taaaggctaa
     1021 tctgacctat cgttggatat ggattccgtc gatatacgga ctcagccctg ccgcggccgc
     1081 atcccaatag agactgtagt tgcaactggt cgaatacccc agcgagaccg gatcgcacac
     1141 gaagatagtc cctccgccac caagaatccc tccgccaagg ttgaaggagt tggtgactga
     1201 taggtagtga tggttattaa tcgggtcgat ccataatccg tcaatattct ccttgtttag
     1261 ccctgcgtct gctccatcga aatggaatgc ccaggttccg gaagtggaat tgccggttgt
     1321 gtcaaacgtg aatgacagca aatcgctttt gcgcgctttg atgatcccgg agggacgaac
     1381 acccactgtc gctctgcctc ttacactaat gatcaacttt ccggaggcgt cgatgctcat
     1441 tgcgtcgatt gcctcggcac gactggtgag atcaacatcg gaaccatcga agtacatttg
     1501 gaagcttcct gaggtcgttg ctcccgtgcc ggttgggaca aagcgaatga tgtcttccgg
     1561 gccaatccga ccgattccgc caataatcag ggctttggtc gggctgagca ggatgctgtt
     1621 gtcggatagc atgataaacg ccttgacacc tgccaatccc agtcccacat ctgagccgtc
     1681 gaagtacatt tgccattgcc ctgccggggt atagcgcaag atgtcgctgt cgtcataggt
     1741 gatgccggcc acgcttccct ttctgctggg gctaacgtag atcgtatcgt ctataggtgt
     1801 gctttccggc gcgttagtga aggtgcagg
//
LOCUS       DAOMQG010000545         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24546_length_1819_cov_8.557823, whole genome shotgun sequence.
ACCESSION   DAOMQG010000545 DAOMQG010000000
VERSION     DAOMQG010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24546_length_1819_cov_8.557823"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLC06_16995"
     CDS             complement(<1..257)
                     /locus_tag="PLC06_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRO92201.1"
                     /translation="MQERYEPVIGLEIHAELLTQSKMFCGCRVVDSVEAAPNTAVCPV
                     CLGMPGMLPVVNGRAVEFAMRVALALGCEIQPHNIFARKSY"
     gene            complement(407..>1819)
                     /gene="gatA"
                     /locus_tag="PLC06_17000"
     CDS             complement(407..>1819)
                     /gene="gatA"
                     /locus_tag="PLC06_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRO92202.1"
                     /translation="ALRRGETTSVAATQAMLDRIVTHDNTIRSYLTVTDELALEQAAA
                     ADRRRAEGEDSPVLGVPIAVKDILCMTGAPTTAGSKILDGFISPYSAYAVDRLVAAGA
                     VILGKTNTDEFAMGSSTEHSAYFTTRNPWDIERVPGGSSGGSAAAVAARLAYGALGTD
                     TGGSVRQPASFCGLVGMRPTYGRVSRWGVIAFASSLDQVGPFGRTAADAAALLGVVAG
                     HDPRDSTSLDAPVPDYEAALTGDIRGLRVGVPREYFVEGMEPEVEAAVRAAIDDLATL
                     GAEIVEISLPHTRYGLPVYYLIAPAEASANLARYDGTRFGPRVVGADMIDTVRQTRAL
                     FGPEVKRRIMLGTYALSAGYYDEYYGRALKVRALIKEDFRRVFESVDVIAAPTCPTTA
                     FRVGDRAGDPLSMYLGDIFTLPLNLSASCGLSVPCGFDSRGLPIGLQIIGDTLQEARI
                     LNVAHAYERVRPWYRQLPVV"
BASE COUNT          345 a          598 c          572 g          304 t
ORIGIN      
        1 aagtagctct tgcgggcgaa gatgttgtgg ggctgaatct cacagccgag agccagggcc
       61 acgcgcatgg cgaactcgac agcgcggccg ttgacaaccg gaagcatgcc cggcatgccc
      121 agacagacgg ggcagacggc cgtgttcgga gccgcttcga cgctgtcgac gacccggcag
      181 ccgcaaaaca tcttcgattg tgtaagcagt tcggcgtgga tttccagacc gatgacgggt
      241 tcataccgct cttgcataac gccttctctc cccaatgacc gacggccaat tgtcccgtgg
      301 ccgccggttg cctgattata acccttccct cgataatgac tcgcggaaag ccgcagagca
      361 acctgccggg ttgccttctt catacctgtg gaccgaacag ttcactctac accaccggca
      421 attgcctgta ccacggccgg acgcgctcat aggcatgggc gacgttcagg atgcgcgctt
      481 cctgcagcgt atcgccgatt atctgcaagc cgatgggcag cccccggctg tcgaacccgc
      541 acggcaccga taacccgcaa ctcgcgctca ggttgagcgg cagcgtgaag atatcgccca
      601 aatacatgct caacgggtcg ccggcccggt cgccaaccct gaacgccgtc gtcgggcagg
      661 tgggcgcggc gatgacgtca acggactcga agacgcgacg aaaatcttcc ttgatcaagg
      721 ctcgtacctt gagggcgcgg ccgtaatatt cgtcataata gccggcgctc agggcatagg
      781 tgcccagcat gatgcgtcgc ttgacctcgg ggccgaagag cgcccgcgtt tgtcgcactg
      841 tgtcgatcat atctgcccca accacgcgcg gcccgaagcg tgtgccgtca tagcgagcca
      901 gattggcgct ggcctcagcc ggagcgatca aatagtagac cggcaagccg tagcgggtat
      961 ggggcaggct gatctcgacg atctccgccc ctaacgtggc cagatcgtcg atggcggcgc
     1021 gaacggccgc ttctacttcc ggctccatgc cctcgacgaa atactctcgc ggcacgccca
     1081 cgcgcagccc gcggatatca cccgtcaggg ccgcttcata gtcgggcacc ggcgcgtcga
     1141 gtgaggtgga atcgcgcggg tcatggccgg ccacaacgcc aagcaaggcg gccgcatcgg
     1201 ccgccgtccg gccaaaaggc cccacctggt caagcgacga ggcgaaggca atgacccccc
     1261 agcgcgagac gcgaccgtat gtaggacgca tgccgacaag accgcagaac gacgccggtt
     1321 ggcgcacgct gccgccggtg tcggtcccca gcgcgccata ggccaggcgc gccgccacgg
     1381 cggcggcgct gccgccgctg ctgccgccgg gaacacgttc aatatcccat ggattgcggg
     1441 tggtgaagta agccgaatgc tccgtcgatg atcccatggc gaactcatcg gtgttggtct
     1501 tgcccaggat gaccgccccg gccgccacca gtcgatcgac ggcataagcg ctataagggg
     1561 aaatgaagcc gtcgagtatc ttgctgccgg cggtcgtcgg cgcgccggtc atgcacagta
     1621 tgtccttgac ggcgatgggc acacccagca cgggagaatc ctcaccctcg gcgcgtcgcc
     1681 ggtcggcggc cgccgcttgc tccagcgcca gctcatcggt gacggtcaga tagctgcgaa
     1741 tggtgttgtc gtgggtcacg atacgatcga gcatggcctg ggtcgcggcc acgctggtgg
     1801 tttcgccgcg ccgaagcgc
//
LOCUS       DAOMQG010000546         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_24987_length_1794_cov_6.610121, whole genome shotgun sequence.
ACCESSION   DAOMQG010000546 DAOMQG010000000
VERSION     DAOMQG010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24987_length_1794_cov_6.610121"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /gene="pheT"
                     /locus_tag="PLC06_17005"
     CDS             <1..>1794
                     /gene="pheT"
                     /locus_tag="PLC06_17005"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRO92203.1"
                     /translation="ATVDYGAAEPKTVVTGAPNLFPYIGRGDLSGEGLYSPLALEGAM
                     LYDGHKEGRVKAKLKGRPLRGIYNDAMLCSEKELGISDEHEGIILITNYELRITNEES
                     TLDSSLVIRNSSFPPGTPLQDILGDAVLEIAILPNTARATSIIGVARELAALIGATFR
                     PPSYDVVMDGPPLDGRVTITTENPELNPRFTAMLIEGVRQIPSPYWMQYRLRLVGQRP
                     INVVVDISNYVMLEVGQPNHTFDYDVLRRRADGYAPDGPIHINTRLPRPGETLTTLDG
                     KTHKLEPFTILVTDPAGNLSVGGIMGGLESEITDATTNVLLEAAAWDFINIRRSANAL
                     DIHTEAGYRFSRGVHPSLALLGAKRAAELLHQLAGGTVAQGIIDTYPRPPAPVVVDLD
                     PTYARKLSGLNLSAAEMGEMLERLGFTVEMVGDKLRATTPDNRMDIEGPHDLVEEICR
                     IYGYDRIPSTRLADELPPQRSNVKLEREERVRNILARLGLQEVWSYRLTSAAREARLL
                     PGTAPDDRPYVTLSNPPAPDRNVMRHSILASVMEATAANSRHAARIALFEIGDVYITG
                     EDELLPDELTRLAIVMTGKRAAAGWGHNDDS"
BASE COUNT          350 a          653 c          527 g          264 t
ORIGIN      
        1 tggccaccgt cgactacggc gcggccgaac ccaagaccgt cgtcaccggc gcgccaaacc
       61 tgttccccta catcggccgt ggcgacctga gcggcgaggg tctctactcc ccgctggcgc
      121 tcgaaggcgc gatgctctat gacggccaca aggaagggcg cgtcaaggcc aagctgaagg
      181 gccgtcccct gcgcggcatc tacaacgacg ccatgctctg ctcggaaaaa gagttgggta
      241 tcagtgacga acatgaaggc atcattttaa ttacgaatta cgaattacga attacgaatg
      301 aagagagcac tctggattcg tcactcgtca ttcgtaattc gtcatttccc cccggcacac
      361 ccctgcagga catcctcggc gatgccgtgc tggagatcgc catcctgccc aacaccgccc
      421 gcgccacctc gatcatcggc gtggcccgcg aactggcggc gctgatcggc gcgaccttcc
      481 ggccgccatc ctatgacgtg gtgatggacg gcccgccgct cgacggccgc gtgaccatca
      541 ccaccgagaa ccccgaactc aacccgcgct tcacggccat gctcatcgaa ggcgtgcggc
      601 agattcccag cccctactgg atgcaatatc gcctgcgact ggtcgggcag cggccgatca
      661 acgtcgtcgt cgacatcagc aactacgtca tgctggaagt cggccagccc aaccacacct
      721 tcgactacga cgtgctgcgt cgtcgcgccg acgggtatgc gcccgacggc cccatccaca
      781 tcaacacccg cctgccccgt cccggcgaga cgctgaccac gctcgacggc aaaacccaca
      841 agctggagcc attcaccatt ctcgtcaccg acccggccgg caacctgtcc gtgggcggta
      901 tcatgggcgg gctggagagc gagatcaccg acgccacgac caacgtcctg ctggaggcgg
      961 cggcctggga tttcatcaac atccgtcgca gcgccaacgc gctcgacatc cacaccgagg
     1021 cgggctaccg cttcagccgc ggcgtccacc ccagcctggc gctgctgggt gcaaaacggg
     1081 cggccgaact gctgcaccaa ctggccggcg gcacggtagc gcaaggcatc atcgacacct
     1141 accccaggcc gcccgcgccg gtcgtcgtcg atctcgaccc cacctatgcc cgcaagctga
     1201 gcggactgaa cctgtcggcg gccgagatgg gcgagatgct ggagcgtctg ggcttcaccg
     1261 tcgagatggt tggcgacaag ctgcgggcca ccacgcccga caatcgcatg gacatcgaag
     1321 gcccccacga tctcgtcgag gaaatctgcc gcatctacgg ttacgaccgc atccccagca
     1381 cccggctggc cgacgagctg ccgccgcagc gcagcaacgt gaagctggag cgcgaggagc
     1441 gcgtccgcaa tatactggcg cgactgggcc tgcaagaggt gtggagctac cggctcactt
     1501 cggccgcccg cgaggcgcgc ctgctgcccg gaacagcgcc cgatgaccgg ccctacgtga
     1561 cgctcagcaa tccgcccgcg cccgaccgca acgtcatgcg ccattcgatc ctggcctcgg
     1621 tcatggaagc cacggccgcc aacagccgcc acgccgcgcg catcgccctg ttcgagatcg
     1681 gcgacgtcta catcaccggg gaagacgagc tgctgcccga cgaactaacc cggctggcca
     1741 tcgtcatgac cggcaaacgc gcggccgccg gatggggcca caacgacgac tcga
//
LOCUS       DAOMQG010000547         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_25195_length_1782_cov_12.903880, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000547 DAOMQG010000000
VERSION     DAOMQG010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25195_length_1782_cov_12.903880"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..956
                     /gene="rsgA"
                     /locus_tag="PLC06_17010"
     CDS             27..956
                     /gene="rsgA"
                     /locus_tag="PLC06_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRO92204.1"
                     /translation="MIKAQSGFFTVQPDDGGEPVVCRIRGRLKQERQRSDIAAAGDRV
                     TFIVNPDGTGLIETVAERVSVLSRTRAAGADSRNLDTDREQVLVANPDQVIFVFSVRN
                     PAFSPRKLDRFLVVAEMNNLPAVICANKVDLVDPGEAREMFRLYEAIGYPVIHTSTLT
                     GEGIDELRERLRGRISVLAGSSGVGKSSLLNAVQEGLGLRVKQVSDATGKGMHTTRHV
                     ELIPLDAGGYVADTPGIRSLALFDLEPEELDAYYREIAPLVAQCQFSDCTHTHEPGCV
                     VLAAVQDGRVSFERYDSYLRLREEQQELTDKKY"
     gene            complement(953..1381)
                     /locus_tag="PLC06_17015"
     CDS             complement(953..1381)
                     /locus_tag="PLC06_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009787123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRO92205.1"
                     /translation="MTYIPSKLRNLVAERAAHCCEYCQSQEVVLGMPFEIEHIVPLAA
                     GGRTEEKNLCLACWRCNRYKGTQVERVDEESLAIVPLYHPRQHVWDEHFSWSNDGLRI
                     TGLTPTGRVTIEALKMNNPFVVRSRYKWVASGWHPVRPNR"
     gene            complement(1381..>1782)
                     /locus_tag="PLC06_17020"
     CDS             complement(1381..>1782)
                     /locus_tag="PLC06_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92206.1"
                     /translation="ITIDLPGPVAEQLEEEAIRQRMSIRELVRELVLEHWSGLPSLPD
                     DVEAELAAFHQLSDDVLWLVARTTLTPEEQRELAELNDTTQSRPLADDEYNRREALLD
                     SYDRMMIRRAQAAHLLQLRGYDLSNPAVLTQ"
BASE COUNT          353 a          535 c          517 g          377 t
ORIGIN      
        1 cacccttgtc cgacccgacc ggcctcatca tcaaagccca aagcggcttc ttcaccgtcc
       61 agcccgacga cggcggggag ccggttgtct gccggattcg cggccgcctg aagcaggagc
      121 ggcagcgcag cgacatcgcc gccgccggcg atcgcgttac tttcatcgtc aatcccgacg
      181 gtacaggtct catcgagacc gtcgccgagc gcgtctcggt cctgtcgcgc acgcgcgcgg
      241 ccggggccga ttcccgcaat ctcgacaccg accgcgagca ggtgctggtg gccaatcccg
      301 atcaggtcat cttcgtcttc tccgtgcgca accccgcctt cagcccacgc aagctcgacc
      361 gctttctcgt cgtggccgag atgaacaact tgccggccgt catctgtgcc aacaaggtcg
      421 atctggttga tccgggcgag gcgcgcgaga tgtttcgtct ctatgaggcc atcggctatc
      481 cggttatcca caccagtact ctgaccggcg aggggatcga cgaactgcgc gagcgactgc
      541 gtggccgcat ctccgtgctg gccgggtcgt cgggcgtcgg caagtcgagc ttgctcaacg
      601 ccgtgcaaga ggggttgggg ttacgtgtga agcaggtcag cgacgccacc ggcaagggga
      661 tgcacaccac gcgccacgtc gaactcattc cgctcgacgc cggcggctat gtggccgaca
      721 cgccgggcat tcgcagcctg gccctgttcg acctggagcc ggaggagctg gacgcctact
      781 atcgggagat cgcgccgttg gtagcccaat gccagttcag cgattgcacc cacactcatg
      841 agccgggttg tgtggtgttg gccgcggtgc aggacggccg cgtgtcgttt gagcgatatg
      901 acagctattt gcggttgcgg gaggagcagc aggaattaac ggataagaag tactagcggt
      961 tgggccttac cggatgccac cccgaagcga cccatttgta ccgtgagcga acaacgaatg
     1021 ggttgttcat cttcaatgct tcaattgtaa cccgtcctgt tggcgtcaat cctgtaatcc
     1081 gcaggccatc atttgaccag gaaaaatgtt catcccacac atgttggcgc ggatgataca
     1141 gagggacgat ggccaggctt tcttcatcaa ccctttcgac ttgcgttccc ttgtatcggt
     1201 tacatcgcca gcaagccagg caaaggttct tctcttcagt cctcccacct gccgcgaggg
     1261 gcactatatg ttcaatctcg aaaggcatgc ctaatacgac ttcctgggat tggcaatatt
     1321 cacaacaatg ggcggcacgc tcggccacaa ggttgcgaag ctttgaggga atataggtca
     1381 ctactgggtt aatacggccg gattggatag atcatatccg cgtaattgaa gtaagtgagc
     1441 tgcttgggcg cgtcttatca tcatgcgatc gtaggagtca agcaacgctt cgcgccggtt
     1501 gtactcgtca tcggccaacg gccgagattg cgtcgtatcg tttagctcag ccagttctct
     1561 ttgctcttcg ggagtcagcg ttgtccgtgc gacgagccac aatacatcat cggacaattg
     1621 atggaacgca gccagttccg cctctacgtc atcgggcaag gaaggcagtc cgctccaatg
     1681 ttccaacacc aactcgcgga caagctcacg aatggacatc ctctgtcgaa tggcttcctc
     1741 ttccaactgc tcagcaactg ggccgggaag gtcgatggtt at
//
LOCUS       DAOMQG010000548         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_25275_length_1777_cov_6.747387, whole genome shotgun sequence.
ACCESSION   DAOMQG010000548 DAOMQG010000000
VERSION     DAOMQG010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25275_length_1777_cov_6.747387"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PLC06_17025"
     CDS             <1..173
                     /locus_tag="PLC06_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176549.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRO92207.1"
                     /translation="KARGNPLGATGLYQIVEVAHQVRGTAGDCQVPGARVGMAQNLGG
                     MAATAVTHILVN"
     gene            399..809
                     /locus_tag="PLC06_17030"
     CDS             399..809
                     /locus_tag="PLC06_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRO92208.1"
                     /translation="MEVSRHWRLNQQRYALVGEVCPNCGVKLFPPRDVCLECEAPAKE
                     LYTFTGLGEVYSYTTIYDPPAGFEHNAPYMVALVKLEEGPLVTAQLTDVDFANVEIGM
                     PVEMVTRKLRTDGEEGMIIYGYKFRPTFANAIAG"
     gene            complement(897..>1777)
                     /locus_tag="PLC06_17035"
     CDS             complement(897..>1777)
                     /locus_tag="PLC06_17035"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRO92209.1"
                     /translation="SSFTLYGVPPTTQIADFAQRDGRWLSEAAADVPFYINYETEKLT
                     GRPAVGEALSLRINGLHDLEAQLVGISSRPFEANAYLPYDAFEQATGETGRAGRLVVY
                     MTSGSSAASGLPSADAAAQDAVAAALVARYEAAGMTVLRTETAAGFREGYHAQFNMLV
                     VLLMSLAGLTAVVGGLGLANTMALNVLERSREIGILRSMGAGRGLLRRLVLAEGLAIA
                     LIGGLLGILLSVPLTVILDRVMGGTLMGSPLSFAFAPWAAAGWLALALLIGLVACWVP
                     AESAARMTIRAALAYE"
BASE COUNT          349 a          612 c          531 g          285 t
ORIGIN      
        1 tgaaggcgcg cggcaacccg ctgggcgcga cgggtctcta ccagatcgtc gaggtggccc
       61 accaggtgcg cggcacggcc ggcgattgcc aggtacctgg cgcgcgcgtg ggcatggccc
      121 aaaacctcgg cggcatggcc gcgacggcgg tgacgcacat ccttgtcaat taggaattgt
      181 gaaggggtga aggggagaga gggagaaagg gggaagggga gatcacccct tcaccccttc
      241 acccctcccc ctgattgatt tggtagtttt tgacagcatt ttccccggtg ctgacagtag
      301 ttgatagtca ggccgtggat aattttcgat gaatacaggc gcggcgcgca tgatcaaatc
      361 ggccatgagc caacccgtcg cctggaggta acgaaaaaat ggaagtatca cgtcattggc
      421 gactcaatca acaacggtat gctctggtgg gcgaggtttg ccccaactgc ggcgtcaagc
      481 tattcccgcc gcgcgacgtc tgcctggagt gcgaagcgcc ggccaaagag ctttacacct
      541 tcaccggcct gggcgaggtc tattcctaca ccaccatcta tgacccgccg gcgggcttcg
      601 agcacaacgc cccctacatg gtggcgctgg tcaagctgga agaaggccct ctggtcacgg
      661 cgcaactgac cgacgtcgat ttcgccaatg tagagatcgg catgccggtg gagatggtga
      721 cgcgcaagct gcgcaccgac ggcgaggaag ggatgatcat ctacggctat aagttccggc
      781 cgaccttcgc caacgcgatc gccggttaac tgaactcaaa cgactttgtt gtaaaacagg
      841 acggtcgttc cccaacgccg aggaacgacc gtcctgtttc tttttactgc tcccgtctac
      901 tcatacgcca gcgccgcccg aatcgtcatc cgcgccgcgc tctcggccgg cacccagcaa
      961 gccaccaagc caatcagcag cgccagcgcc agccagccgg cggccgccca cggcgcgaag
     1021 gcaaacgata gcgggctgcc catcagcgtc ccgcccatca cccgatcaag gattaccgtc
     1081 aacggcaccg acagcaggat gcccaacagg ccgccgatta gagcgatagc cagcccctcc
     1141 gccagcacca gccgccgcaa cagcccgcgc cccgcgccca tcgagcgcag gatgccgatc
     1201 tcccgcgatc gctccagcac gttgagagcc atcgtattgg ccagccccaa cccgccgacc
     1261 accgctgtca gccccgccag cgacatgagc agcacgacca gcatattgaa ttgcgcgtgg
     1321 tagccctcgc ggaacccggc ggccgtctcc gtgcgcagca ccgtcatccc cgccgcctca
     1381 taccgcgcca ccagtgccgc cgcgaccgcg tcctgcgccg ccgcatcggc ggacgggagt
     1441 ccgctggcgg ccgaggaccc gctcgtcatg tagaccacca gtcgcccggc gcggcccgtc
     1501 tcgcccgtgg cctgctcaaa cgcgtcgtag ggcagatagg cgttggcctc gaacggccgc
     1561 gagctgatgc ccaccagctg cgcctccaga tcatgcaggc cgttgatccg cagcgacagc
     1621 gcctcgccca ccgccggtcg tccggttagc ttctccgtct catagttaat atagaaagga
     1681 acgtcggcgg ccgcctcgga aagccatcga ccgtcccgct gggcgaaatc ggcgatctgc
     1741 gtcgtgggcg gcacgccgta gagcgtgaag ctgctgc
//
LOCUS       DAOMQG010000549         1774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_25333_length_1774_cov_11.014543, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000549 DAOMQG010000000
VERSION     DAOMQG010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25333_length_1774_cov_11.014543"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLC06_17040"
     CDS             <1..588
                     /locus_tag="PLC06_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HRO92210.1"
                     /translation="AELVGEGRNIVVNPAKPSEYGDNDLVVYINPTGRFVVGGPMGDA
                     GLTGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGLADRCEVQ
                     VAYAIGVAHPLSVNVETFGTSQYTNEQLVALINEQFDLRPGAIIRDLKLRRPIYRATA
                     AFGHFGRDDIEAPWENTDRAEALRVSAERLQPVFA"
     gene            744..>1774
                     /gene="ffh"
                     /locus_tag="PLC06_17045"
     CDS             744..>1774
                     /gene="ffh"
                     /locus_tag="PLC06_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRO92211.1"
                     /translation="MFETLGSRLQSVFDNLQKRGKLTEADVDIAMREVRLALLEADVN
                     FKVVKDFVARVRERAIGQEVMRSLTPGQQVVKIVHDELINTLGEPGRLNLGAQSPAVI
                     MLVGLQGAGKTTMAGKLALHLRKQGRRPLLVAADVYRPAAINQLETLGKQLDIPVYSE
                     GINANPVNVCVNGVKQAVATGATTVILDTAGRLNIDEMMMGEIKGIKARVQPIETLLV
                     ADAMTGQEAVRVATDFHQAVTLTGLIMTKIDGDARGGAAISMREVTGVPIKFLGTGEK
                     LNAIEVFHPDRMASRILGMGDVMTLIEQAQSEMDMEEAQRAGAKLASGEFDLDDFLKQ
                     LQQVKKMGP"
BASE COUNT          378 a          467 c          568 g          361 t
ORIGIN      
        1 gcggaactgg ttggtgaagg gcggaacatc gttgttaacc cggccaagcc gtcggagtat
       61 ggagataacg atctggtcgt ctatatcaac ccgaccggtc gatttgtagt tggtggccca
      121 atgggcgatg ccggcctgac cgggcggaag atcatcgtcg atacttatgg cggcatgggc
      181 cggcatggag gcggagcgtt cagcggcaag gatccgacga aggtggaccg tagcgcggcc
      241 tatgcggcgc gatgggtggc gaagaatctc gtcgctgccg gtctggccga tcgttgcgaa
      301 gtgcaggtgg cttacgccat cggcgttgcc cacccgctca gcgtcaatgt tgagaccttt
      361 ggcacgagcc aatacacgaa tgagcaactg gtggccctga tcaacgagca gttcgatctg
      421 cggccgggcg ctatcattcg tgatctgaaa ctgcgccggc ccatttatcg ggcgacggcc
      481 gccttcggtc actttggccg tgatgatatc gaagctccgt gggagaatac cgatcgagcc
      541 gaagcattgc gcgtatcggc cgagcgtctg cagcccgttt ttgcgtaatt gcgatcgcgg
      601 gtttagaatc acaaaatata gaatgacgaa gcccatccgc cttgagcggg tgggctttga
      661 cgtgtgccga cggcttgacg ctatgccgtg ggtgcgttag aatccaaaac cttgcaaact
      721 gttcgttgca tgatggtggc tttgtgtttg agactctagg atcgcgatta cagtccgttt
      781 tcgacaacct gcaaaagcgc ggcaaattaa cggaagctga tgttgacatc gcaatgcgcg
      841 aggtccgtct ggcgctgctt gaggccgacg tcaacttcaa ggttgttaag gattttgtcg
      901 cccgggtcag agagcgggcc attggccagg aagtgatgcg cagcttgacg cctgggcagc
      961 aggtcgtcaa gatcgttcac gatgagctga tcaacacgtt aggtgagcct ggacggttga
     1021 acctgggtgc acaatcgccc gcagtcatta tgctggttgg gttgcaaggt gcgggaaaga
     1081 cgacgatggc cggcaagctg gcgctccact tgcggaagca aggcaggcgg ccgcttctcg
     1141 tggctgccga cgtctatcgc ccggcggcca ttaaccaatt ggaaacgcta gggaagcaac
     1201 tggacattcc cgtctacagc gaaggcatca atgccaaccc cgttaatgtg tgtgtcaatg
     1261 gggtgaagca agccgttgcg acgggggcga cgacggttat tctcgatacc gccggtcgtc
     1321 tgaacatcga cgaaatgatg atgggcgaga tcaagggcat caaagcccgc gtccaaccca
     1381 tcgagacgtt gctcgtggcg gatgccatga ctggccagga agcggtgcgc gttgccaccg
     1441 acttccacca ggccgtcacc ctgactggtc tgatcatgac caagatcgac ggtgacgcgc
     1501 gtggtggcgc ggccatctcc atgcgcgagg tcaccggcgt cccaatcaaa ttcctcggca
     1561 cgggcgaaaa gctgaacgcc atcgaggtgt tccatcccga ccgtatggcc agccgtatcc
     1621 tgggaatggg cgatgtgatg accctcatcg aacaggccca atccgagatg gatatggagg
     1681 aggcccaacg cgccggcgca aaactggcgt cgggcgaatt tgatctggat gatttcctga
     1741 agcaattgca gcaggtcaag aagatggggc caat
//
LOCUS       DAOMQG010000550         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_25385_length_1771_cov_7.322261, whole genome shotgun sequence.
ACCESSION   DAOMQG010000550 DAOMQG010000000
VERSION     DAOMQG010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25385_length_1771_cov_7.322261"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1630)
                     /gene="aroB"
                     /locus_tag="PLC06_17050"
     CDS             complement(2..1630)
                     /gene="aroB"
                     /locus_tag="PLC06_17050"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRO92212.1"
                     /translation="MTDNNIILTGFMGTGKTTVGRLLAAQLGRPFVDTDDLIVERDGR
                     SVADIFNEDGETRFRELEAKVAADLAQRRGLVVATGGRLMLDPANAAALGATGPVFCL
                     AAEPAAILERVAAGGEPRPLLAVDDPERRVMALLQRRAAAYARFRAVETDGQSPQQIA
                     ATVAALAANGLRQAIPVRHPTGRYDVIVGENLLGSVLDLAGIDGPAAIITDSHVGPLH
                     AAKIRKSGPHGIPILIMHAGEQHKNLDTVRDLYDGLSAAGIGRDGTIIALGGGVAGDV
                     AGFVAATWLRGIEFVLCPTSLLAMVDSSIGGKTGVDLPQGKNLVGAFHQPRQVLADIG
                     TLATLPAGEFTAGLAEVAKHGLIADRILWQRLMMEEWHFDPRRLSEDRLLRADLHSLV
                     TRAIEVKRRVVEEDPYEQGRRALLNLGHTFGHAVEQVSGYAIRHGEAVAMGLAAAAHL
                     SAALGECSPSLPGLVEMVLTRLGLPIHIPPLVNGRSLDPAALFAAMGSDKKKKAGRLR
                     FVLIHDIGDVFIRDDVSEAAVLAALEAARMTTEE"
BASE COUNT          279 a          620 c          573 g          299 t
ORIGIN      
        1 gttactcttc tgtcgtcatt cttgccgctt ccagcgccgc cagcacggcc gcttcagaca
       61 cgtcgtcgcg gatgaaaacg tcgccgatgt cgtgaataag cacaaagcgc agccgcccgg
      121 ctttcttctt cttgtcgctg cccatggccg cgaagagcgc cgccggatcc agcgaacggc
      181 cgttcaccaa cggcggaatg tggatcggca ggcccagccg ggtcagcacc atctccacca
      241 gccccggcag tgagggcgag cactccccca gtgcggccga cagatgggcc gcagcggcca
      301 gccccatggc caccgcctcg ccgtggcgga tggcatagcc cgacacctgc tccaccgcgt
      361 ggccgaaggt gtggcccaga ttgagcaacg cccgccgtcc ctgctcatac gggtcttctt
      421 ccactacccg ccgcttcacc tcgatggccc gcgtcaccag cgaatggaga tcggcccgca
      481 gcagccggtc ttcgctcagc cgccgcggat cgaagtgcca ttcttccatc ataagccgct
      541 gccaaagtat ccggtcggcg atgaggccgt gcttggccac ctcggccagc ccggcggtga
      601 actccccggc cggcagcgtg gccagcgtgc cgatatcggc caacacctgc cgcggctggt
      661 ggaacgcgcc gaccagattt ttaccctgcg gcagatcgac gccggttttg ccgccgatgc
      721 ttgaatcgac catcgccagc agcgacgtgg gacaaaggac gaactcgatg ccgcgcagcc
      781 aggtagcggc gacaaacccg gccacatcgc cggccacgcc gccaccgaga gcgatgatcg
      841 tcccgtctcg gccgatgcct gcggccgata atccgtcgta gagatcgcgc acggtgtcga
      901 ggttcttgtg ctgctcgccg gcatgcatga tcaggatggg aatcccatgg ggacccgatt
      961 tccgaatctt cgccgcgtgc agcggtccga cgtgactgtc ggtgatgatc gccgccgggc
     1021 cgtcgattcc agccaggtcg aggacgctgc ccagcagatt ctcgcccacg atcacgtcgt
     1081 agcggcccgt cggatggcgc accggaatcg cctgccgcag cccgttggcg gccagtgcgg
     1141 cgacggtcgc ggcgatctgt tgcggcgatt ggccgtcggt ctccacggcg cggaagcggg
     1201 cataggcggc ggcgcggcgc tggagcagag ccatcacccg ccgctcggga tcatcgacag
     1261 ccagcagcgg ccgcggttcg cccccggcag ccacgcgctc caggatggcc gccggctcgg
     1321 cggcgaggca gaagaccggc ccggtggctc ccagtgcggc ggcgttggcg gggtcgagca
     1381 tcagccggcc gccggtggcg acgaccagcc cgcgccgctg ggccagatct gcggcgactt
     1441 tggcttccag ctcgcggaag cgggtctcgc cgtcctcgtt gaaaatgtcg gcgaccgaac
     1501 ggccgtcgcg ctcgacgatg agatcgtcgg tgtcaacaaa cggccgcccc agttgcgccg
     1561 ccagcaggcg gccgacggtc gttttgcccg tccccatgaa tccggtgagg atgatgttgt
     1621 tgtcggtcat gtcgtgtcga ttccgccgcc tgtcggcgcc ttgtgtgtat tatccactat
     1681 tttccaccag tgtgggaacc aactgcggag gccggccttt ttgtttctcc ggcggccgcg
     1741 attatagccg ctcggccatc gacccggcgt a
//
LOCUS       DAOMQG010000551         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_25856_length_1742_cov_5.053349, whole genome shotgun sequence.
ACCESSION   DAOMQG010000551 DAOMQG010000000
VERSION     DAOMQG010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25856_length_1742_cov_5.053349"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..642
                     /locus_tag="PLC06_17055"
     CDS             130..642
                     /locus_tag="PLC06_17055"
                     /inference="COORDINATES: protein motif:HMM:NF039927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HRO92213.1"
                     /translation="MTSERPLIPCTGCGALVPNEDGPTHRYVGASPGCWAIHGEVLAR
                     GYSAPGFGDELMLQINSYCVQHPGTPSPQSIQSVCVHLIGLYLVLERNFDGRRALEAV
                     RAAADGSRAFHWLEPPTHHYEVTILDIHRAGDPAAYAAALTEMARLTWAAWSNHHSQV
                     RSWAAELGLY"
     gene            655..1431
                     /locus_tag="PLC06_17060"
     CDS             655..1431
                     /locus_tag="PLC06_17060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO92214.1"
                     /translation="MDIQTVLALYNHEQRREAEYPDMTRVAMPRIVRYERAAPGTSII
                     LYSDLDETTADADIDEQLAYFSRNRMPFEWKAYACDRPADLVDRLVARGLAADEPDAI
                     MVLDVAAAPDTLLRRPSADVRRLTDAAQLADVVRVLEPVWGGKFDWIHTRLGAHMAIP
                     DYLSVYVAYEAGLPACAGWTYYNRGHFAGLWGGSTVADYRGRGHYMAVLAARVREARE
                     RGVAYLTVDAGSMSKPILTRHGFEVIAWATACNWEPAETA"
     assembly_gap    1517..1526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1527..1722)
                     /locus_tag="PLC06_17065"
     CDS             complement(<1527..1722)
                     /locus_tag="PLC06_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92215.1"
                     /translation="MNLLLYTEARMKLVRRDEEPTTVQPTGLLDDMDITLPDSLFAEL
                     GYTGALDTDEAGAGPTDPPQW"
BASE COUNT          312 a          546 c          542 g          332 t
ORIGIN      
        1 tggcgcattg cagagcgatt tgcccgccca tggtgcccgc gccgatgatg agaacctgtt
       61 tgatgtctgc tgcgttcatg cttgcttttc tgtttccggt caatgatata atagcgccgg
      121 acaaacgaca tgaccagcga gcgaccactc atcccctgca ccggctgcgg cgcgctcgtc
      181 ccaaacgagg atggcccaac ccaccgctac gtcggcgcgt cgcccggctg ctgggcgatc
      241 cacggtgagg tgctggcccg cggctatagc gcgcccggct tcggcgatga gctcatgctg
      301 cagatcaata gctactgcgt tcagcacccc ggcacgccgt cgccccagtc gatacaatcc
      361 gtctgcgtcc atctcatcgg cttgtacctc gtccttgagc gaaactttga cggcaggcga
      421 gcgctggaag ccgtccgcgc cgcggccgac ggcagccgcg ccttccattg gctggagcca
      481 ccaacccacc attacgaggt gaccatcctc gatattcatc gcgccggcga cccggccgcg
      541 tatgccgctg ccctgacgga gatggcgcgg ctcacctggg cggcgtggtc gaatcatcat
      601 tcgcaggtgc gaagctgggc ggccgaattg gggctgtatt gattgaagga gataatggac
      661 attcaaaccg tattggctct ctacaaccac gagcaacgac gcgaggctga ataccccgac
      721 atgacacggg tcgccatgcc ccgcatcgtg cgctatgagc gagccgcacc cggcacgagc
      781 attatccttt atagcgacct ggatgagacc acggccgacg cggatatcga cgagcaactc
      841 gcgtattttt cgcgcaacag gatgccgttt gaatggaaag cctacgcctg cgatcgcccg
      901 gccgatctgg tcgacaggct ggttgctcgc ggtctggcgg ccgacgaacc ggacgcgatc
      961 atggtgctcg atgtggccgc cgcgcccgac accctgctac gcaggccgtc ggccgatgtg
     1021 cgccgcctga ccgatgcggc ccaactcgcc gatgtcgtca gggtgctgga gccggtgtgg
     1081 ggcggaaagt tcgactggat tcacacgcgc ctcggcgccc atatggccat ccccgactat
     1141 ctcagcgtct atgtggctta tgaggccggt ctgcctgcct gcgcgggttg gacctattat
     1201 aaccggggtc atttcgccgg tttgtggggt gggtccactg tcgcggatta ccggggacgc
     1261 ggccactaca tggccgtatt ggccgcgcgt gtgcgagaag caagagagag aggggtagcc
     1321 tacctgaccg ttgacgccgg ttcgatgagc aagccgattc tgacccgcca tggcttcgag
     1381 gtcatcgcct gggccacagc ctgcaactgg gagcctgcgg aaacggccta agcctggcgc
     1441 aaagcgccga acatcggctc ggcaaaagcc gcgaacagcg agtcgaactc actgtcgaga
     1501 tgggggtcgt cggtcgnnnn nnnnnnccca ttgcggcgga tcggtcgggc cggcgccggc
     1561 ctcatccgtg tccagcgcgc cggtatagcc caactccgcg aacaaactat cgggcagggt
     1621 gatgtccatg tcgtccaaca atccggtcgg ttggacggtg gtcggctctt cgtctcgccg
     1681 gacgagtttc attctggctt cggtgtagag taacaggttc atggccggct ttccaaatta
     1741 aa
//
LOCUS       DAOMQG010000552         1729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26101_length_1729_cov_9.547192, whole genome shotgun sequence.
ACCESSION   DAOMQG010000552 DAOMQG010000000
VERSION     DAOMQG010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26101_length_1729_cov_9.547192"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLC06_17070"
     CDS             <1..398
                     /locus_tag="PLC06_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRO92216.1"
                     /translation="ISAVIEEVGDYWDVLIEGELYAVKVQDERAYRLEHMRATTVSMS
                     GDATVFSPMPGIIIAVPVSVGDRVHHGDKVVILESMKMENELRAPRDGVVSQILVSPG
                     ASVEKDQPLVVITQPDDDALLQTSEAEAE"
     gene            395..1072
                     /gene="cyaB"
                     /locus_tag="PLC06_17075"
     CDS             395..1072
                     /gene="cyaB"
                     /locus_tag="PLC06_17075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRO92217.1"
                     /translation="MSSVVDDGDILSLTPLPQSAAGEDRHLEVEVKFMLRDLAPIRGR
                     LVDMGAEIIAPRVFERNVRFDTADEALLARQQLLRLRQDTRVRLTFKGPAAEDVASEA
                     KVREEIELEVGHFDRMSAVLQRLGFLPIQVYEKYRETYQWRGVEIVLDEMPFGDFIEL
                     EGVEADLRPAAEALGLDWSRRILANYLALMELCRRSFNLPFSDLTFENFAGLSVDMGE
                     LLPISAL"
     gene            1084..>1729
                     /locus_tag="PLC06_17080"
     CDS             1084..>1729
                     /locus_tag="PLC06_17080"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRO92218.1"
                     /translation="MINDERPDLSDIPADVVAYIEALEARLQERGEGTRPNRPAALPD
                     ELPTTKVILTISRDGIIKRTPRHLYGRQRRGGMGIFDLDVPAGDSPAQLAHADAEDTL
                     ILLTDDGRAFRLPLSSISESPVRARGQALRDLLPLRPNERIVGSLPEGGGQYVILLSK
                     RGWVRRVQAVYLGKNLIPGTTFHDLKEGGPLVAACWSAGAGDLFLASREGKGIRF"
BASE COUNT          328 a          462 c          558 g          381 t
ORIGIN      
        1 ctatctcggc tgttatcgag gaagtgggcg actattggga cgtcctcatc gaaggcgagc
       61 tttacgccgt caaggtgcaa gacgagcgtg cctatcgttt ggagcatatg cgcgctacga
      121 ctgtcagcat gagtggcgac gcaactgtgt tctcgccgat gccgggcatt atcatcgccg
      181 tgccggtctc tgtcggcgac agggtgcatc atggcgataa agtcgtcatt ctggagtcga
      241 tgaaaatgga gaacgagctg cgcgcgccgc gtgacggtgt ggtcagccaa atcctcgtgt
      301 cccccggcgc gagtgtggaa aaggatcagc cgcttgtggt cattacccag ccggacgatg
      361 atgcgctcct ccagaccagt gaggctgaag ctgaatgagt tctgtcgtgg atgatggcga
      421 tatcctgtcg ctcaccccgt tacctcaatc tgcggcagga gaagatcgac atttggaggt
      481 ggaggtcaag ttcatgctgc gggatttggc ccccatcagg gggcggctgg ttgatatggg
      541 ggcagagatc atcgcgccac gcgtattcga gcgcaacgtg cgctttgata cggccgatga
      601 ggcgctcctg gctcgacagc aattattgcg cttgcgacag gacacgcgcg ttcgcctgac
      661 gttcaaaggc ccggcggccg aggacgtggc gagcgaggcc aaagtgcgcg aggagattga
      721 gctggaagtc ggtcacttcg atcggatgtc ggccgttctc cagcgtctgg gttttctccc
      781 gatccaggtc tacgagaaat atcgcgagac ctatcaatgg cgcggcgtgg aaattgtgct
      841 ggatgaaatg ccgtttggcg attttatcga gctggaaggg gtggaggccg atctgcggcc
      901 ggcggccgag gcgttagggc tggattggtc gcggagaatt ttggccaact acctggctct
      961 gatggaactc tgccgccggt catttaacct gccgttttcc gatttaacct tcgagaactt
     1021 cgctggctta tccgttgaca tgggcgagct gttgccgata agcgctttat aggaagtaga
     1081 agcatgatca atgacgagag acccgatctc agcgatattc ccgcggatgt ggtggcctat
     1141 attgaggcgc tggaagctcg gctgcaggag agaggcgaag gcactcgccc caatcgaccg
     1201 gcggcgctgc cggatgagct gccgacgacg aaggtcatcc tcacgatcag tcgtgacggg
     1261 ataatcaagc gcacgccccg ccatctctac ggtcgtcagc gtcgtggcgg catgggtatt
     1321 tttgatttgg acgtgccggc cggcgattct cccgcacagc tggcccatgc cgacgccgag
     1381 gacacgctca tattgctcac cgatgacggg cgtgcttttc gacttccact gtcgtcgatc
     1441 tccgaatccc ctgtccgcgc cagaggccag gcgctgcgcg atctgctccc gctgcggccg
     1501 aacgaacgta ttgtaggctc cttgcccgaa gggggtggcc aatatgttat tttgctgagc
     1561 aagcgcggct gggtgcggcg tgttcaggcc gtctatctgg gaaaaaacct catccccggc
     1621 actacgtttc acgatctgaa ggaaggcggc cccctggttg ctgcttgctg gtcggccggt
     1681 gcgggcgatc tgtttttagc ctccagagaa gggaagggaa ttcgtttta
//
LOCUS       DAOMQG010000553         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26187_length_1725_cov_6.581437, whole genome shotgun sequence.
ACCESSION   DAOMQG010000553 DAOMQG010000000
VERSION     DAOMQG010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26187_length_1725_cov_6.581437"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLC06_17085"
     CDS             complement(<1..482)
                     /locus_tag="PLC06_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HRO92219.1"
                     /translation="MRNQVPFWLHLLAIVALLVGFASALVMAWDVSRHPQHMWIMNVV
                     WPVTGLFGGPLTLWAYFRYGRLATRASVMGARERGEKPPNQRLTPFPVMVGKGTLHCG
                     SGCTLGDILAEWLAFGVPTIAVWLGWQSIFSEKIFAVWILDFIFAFAFGIVFQYFTIK
                     P"
     gene            complement(475..1422)
                     /locus_tag="PLC06_17090"
     CDS             complement(475..1422)
                     /locus_tag="PLC06_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023004541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRO92220.1"
                     /translation="MARNMVQFQKGLSEPEFERQFGTEEQCRAAVTAARWPDGFACPA
                     CGGRRHSEVRTRGLLQCSSCRRQTSIIAGTIFASTKLPLRIWFRAIYHLTQSKQGISS
                     IELGRRLGVTQNTAWMLKHKLAQVMMERDQAKRLGGRVEIDDAYLGGERNGGKRGRGA
                     PGKTPFVAAVETTPSGKPHKLKLSRVEGFSTKAISGFASRHLDPGSLVISDGLRAFVG
                     VTEAGCDHQTIRTGSGKAAAQNPAFKWVNTALGNIKAAISGTYRSINSKHVPRYLAEF
                     EYRYNRRYDLGAMLPRLNWAAVRTPPMPYRLLKLAEVYA"
BASE COUNT          301 a          570 c          569 g          285 t
ORIGIN      
        1 ggcttgatgg tgaagtactg gaagacgatg ccgaaagcga aggcgaagat gaaatccagg
       61 atccagacag cgaagatctt ctccgagaag atcgactgcc agccgagcca gaccgcgatc
      121 gtcggcacgc cgaaggccag ccactcggcc aggatgtcgc ccagggtgca gccgctgccg
      181 cagtgcagcg tccccttgcc gaccatgacc gggaagggcg tcagccgctg gttgggcggc
      241 ttctcgccgc gctccctcgc acccatgacg ctcgccctgg tcgccagccg cccgtagcgg
      301 aaataggccc agagcgtcag cggcccgccg aagaggccgg tcaccggcca caccacgttc
      361 atgatccaca tgtgctgcgg gtgacgcgac acgtcccagg ccatcaccag cgccgaggcg
      421 aagccgacga ggagggcgac gatggcgagc aggtgaagcc aaaaaggaac ctgattacgc
      481 ataaacctca gccagcttca acagccgata cggcatcggc ggggtgcgga cagccgccca
      541 attgagcctg ggcaacatgg cgccgagatc gtagcggcgg ttgtatcggt actcgaactc
      601 ggcgagatag cgcgggacgt gcttgctgtt gatcgaccgg tatgttccgc tgatcgccgc
      661 cttgatgttg ccgagcgccg tgttcaccca tttgaaagcc gggttttgtg ccgccgcctt
      721 gcccgagccg gtgcgaatgg tctgatggtc gcagcccgcc tcggtcacgc caacgaaggc
      781 ccgcaggccg tcgctgatga ccaggctgcc cggatcgaga tgccggctgg cgaagccgga
      841 tatcgccttg gtggagaaac cctcgacccg actgagcttg agcttgtgcg gcttgcccga
      901 gggcgtggtc tcgacggccg ccacgaacgg cgtcttgccg ggggcgccgc gaccacgctt
      961 gccgccattg cgctcgccac cgagataggc atcgtcgatc tcgacccgtc caccgagccg
     1021 cttggcctga tcccgctcca tcatcacctg ggccagcttg tgcttgagca tccaggccgt
     1081 gttctgggtc acgcccagac gccgacccag ctcgatgctg gagatgccct gcttgctctg
     1141 ggtcagatga tagatcgcac gaaaccagat ccgcagcggc agcttggtcg aggcgaagat
     1201 cgtgccggcg atgatcgacg tctgccggcg gcagctggag cattgcaaca ggccgcgcgt
     1261 ccgaacctcg ctgtgccgcc gcccgccgca tgccgggcag gcaaaaccat ctggccagcg
     1321 cgcggcggtc acggccgccc ggcattgctc ttccgtgccg aactgccgct cgaactcggg
     1381 ctcgctgagc cctttctgga attgcaccat gttccgcgcc atcgttgcgc tccctcaagc
     1441 acttgaatgt tcttgctttg taccacatgc cgggcggctg agaaagctgc gtaatcaggt
     1501 atgttaatga cagcagcgcc ggtctctggc gattcgaggc gcgagcaaag cagtgctccg
     1561 gtcggacagg gcaggaaacc aacgggatgt ggcgcagcct gtcgtctggg cacggcgcga
     1621 cggcggctcg gcttgcgcgg ctcgccgccc tcgtcctcct cctggccgcc ggcatcgggg
     1681 cggtctgggc ctggcgggcg gagcggcgca tcgccgaggc gcgcg
//
LOCUS       DAOMQG010000554         1718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26306_length_1718_cov_5.915213, whole genome shotgun sequence.
ACCESSION   DAOMQG010000554 DAOMQG010000000
VERSION     DAOMQG010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26306_length_1718_cov_5.915213"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>1718)
                     /locus_tag="PLC06_17095"
     CDS             complement(79..>1718)
                     /locus_tag="PLC06_17095"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HRO92221.1"
                     /translation="ESAHIDGCLYHGYSGLEFAERLVAGGAQVAVPTTLNVGAMDLIN
                     PEIFRGDAQTGQWATNLMQAYEAMGCKPTFTCAPYQAQHRPSLGTQIAWAESNAIVFA
                     NSVLGARTNRYGDFIDICCAITGRAPDVGLHRPENRAGQILFRLQGIPERLLREDVLF
                     PVLGYWLGAHTGTKIPVIEGLLPETTEDQLKIMGAAAASSGGVALFHAVGITPEAPTL
                     EAAFRGRDPEAVVEVTLDDLKGTLGILSTAADGPIDVVALGSPHFSMTEFAQLLPLVE
                     RFPPRSGIEFIVCTDRLAQVTLHRRGWLERLRAAGVQIVADTCILGTPIVRAQDGVLM
                     TNSGKYAHYTPGGIGLQVVYGSLEECVRSAAAGEVWRDAGLWAGEQGRKGAGGSAQYS
                     VSRYSAGQSQIGGQSSVSAGRALVDGEASGPALVLDAPLSLWGGLDPVTGNIIDQRHP
                     QWGTNVAGRMLLMPVGRGSSSASSILLEATRLGTAPAAILLAEPDAILALGAAVAREL
                     YGAAPPVVVLDEESYRRITDGVMVRVTHGDIQLGNHQ"
BASE COUNT          316 a          601 c          506 g          295 t
ORIGIN      
        1 cgagggagtc cgttttgtca atccttttgg ccgcgatttc gatctccaaa catggattgg
       61 cgacgaccgg gagatatcct actgatggtt ccccaattga atgtcgccat gagtgaccct
      121 caccataacc ccatccgtga ttcggcgata gctttcttca tccagcacca ccaccggcgg
      181 ggcggctcca tacagctccc gtgcaacggc cgcgccaagg gccagaatcg cgtccggctc
      241 ggccaataga atcgccgccg gagccgtacc cagtcgcgtc gcctccagca agatgctgct
      301 ggcgctgctg ctgcccctgc ccaccggcat gagcaacatc cggccggcga cgttcgttcc
      361 ccattgcggg tgtcgctggt cgatgatgtt ccccgtcacc ggatccagac cgccccacaa
      421 gctgagcggc gcgtcgagca ccagcgccgg gccggaagct tcaccgtcaa ccaaagcgcg
      481 gccggcgctt accgacgact gaccgccaat ctgcgactga cctgccgaat accggcttac
      541 tgaatactgc gcactccccc ctgctccttt tctcccctgc tcccctgccc aaagccccgc
      601 atcacgccac acctctcccg ccgcggccga acgcacgcac tcctccaggc tgccatagac
      661 gacctgcaag ccgattccgc cgggcgtgta gtgggcatac ttgccggagt tggtcatcag
      721 cacgccgtcc tgcgcccgca cgataggcgt gccgagtatg caggtgtcgg cgacgatctg
      781 cacgcccgcg gcccgcaacc gctccagcca gccccggcga tggagcgtca cctgagccag
      841 ccggtcggtg cagacgatga actcgatacc cgatcgcggc gggaaccgct ccaccagcgg
      901 caagagttgg gcgaattcgg tcatcgaaaa atgggggctg cctaatgcaa ccacgtcgat
      961 cgggccgtcg gccgcggtgg acaggatgcc gagcgtgccc ttgagatcat caagcgtgac
     1021 ctcgacgacc gcctccgggt cgcgtccccg gaaggcggct tcaagcgtcg gcgcttccgg
     1081 cgtaatcccg acggcgtgga acagagccac accacccgac gaagccgcgg ccgcgcccat
     1141 gatcttgagc tgatcctcgg tggtctccgg caataatcct tcgatgacgg gtatcttcgt
     1201 ccccgtatgc gcccccagcc agtagcccag caccgggaac agcacgtcct cgcgcagcaa
     1261 tcgttcgggg atgccttgca atcgaaacag gatttgcccg gcgcgattct ccggtcgatg
     1321 aaggccgaca tccggggcgc ggccggtgat ggcgcaacaa atatcaatga aatcgccgta
     1381 gcggttggtg cgcgcgccaa gcacggagtt ggcaaagacg atggcgttgc tctcggccca
     1441 ggcgatctgc gtgcccaacg acggccgatg ctgggcctga taaggcgcgc aagtgaacgt
     1501 tggcttgcac cccatcgcct cataagcctg catcaggttc gtggcccact gccccgtttg
     1561 cgcgtcgccg cgaaatattt cggggttgat caggtccatc gcccccacat tgagcgtggt
     1621 cggcacggcg acctgcgctc cgccggcgac cagtcgctcg gcgaactcca gcccggaata
     1681 gccgtgatag agacagccgt cgatgtgggc cgattcga
//
LOCUS       DAOMQG010000555         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26722_length_1698_cov_9.065125, whole genome shotgun sequence.
ACCESSION   DAOMQG010000555 DAOMQG010000000
VERSION     DAOMQG010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26722_length_1698_cov_9.065125"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLC06_17100"
     CDS             <1..869
                     /locus_tag="PLC06_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRO92222.1"
                     /translation="LGRLRAIPHVEIIRIGSRVPVFLPMRITPEFCDMVEKYHPFWIN
                     IHVNHPKEITPELAAATDRLSRAGVPLGNQSVLLAGINDSVHIQRKLVHELVKIRVRP
                     YYLYQCDLVEGSGHLRTTVAKGIEIMEGLRGHTSGYAVPTYVVDAPGGGGKIPVMPNY
                     VISQAPGKVVLRNFEGFITTYTEPLDYDPHEIRDQEALIAPRPEPGQEGVLALLEGER
                     MSIEPSGFASTHARGGGEHRLRSGDISGKWQPLGVGSIEVHKPRERLNEGKAHEAHEA
                     HNEKETEPPAQG"
     gene            complement(911..>1698)
                     /locus_tag="PLC06_17105"
     CDS             complement(911..>1698)
                     /locus_tag="PLC06_17105"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRO92223.1"
                     /translation="GIKLGAKIDLMDDAWWGPSSLPPDASVMFHVGERSYPGAIMVNA
                     AGKRFTNEAASYVEVGHAMYENHTIENRHIPATFIMDSRYRRKYIFGTLFPMQPVPDA
                     YFESGYFKRADTLEELAEQCDIDPAGLRETVERFNRFARSGVDEEFHRGDSAYDRYYG
                     DPTVKPNPCLAPIEKPPFYAVEMVPGDLGTKGGLVMDEYARVLREDGSVIEGLYVTGN
                     NSASVMGNTYPGAGSTIGPSMTFGYIAAKHLAGGKIRAAGDPP"
BASE COUNT          303 a          541 c          557 g          297 t
ORIGIN      
        1 tcctcggccg cctgcgggcc atacctcatg tggagatcat ccgcatcggt tcgcgcgtac
       61 ccgtcttcct gcccatgcgc atcacgcctg aattctgcga catggtggag aaatatcatc
      121 cgttctggat caacatccac gttaaccacc ccaaggagat cacccctgag ctggccgcgg
      181 cgaccgaccg gctgtcgcgc gcgggcgtgc cgctgggcaa ccagagcgtg ctgctggccg
      241 ggatcaacga ctccgtccac attcagcgca agctggtgca tgagttggtg aagatccgcg
      301 tgcggccgta ttacctctac cagtgcgacc tggtcgaggg cagcggccat ctgcgcacga
      361 cggtcgccaa ggggatcgag atcatggagg ggctgcgcgg ccacacgtcg ggctatgccg
      421 tgcccaccta cgtcgtcgac gcgccgggcg gcggcggcaa gatcccggtc atgcccaact
      481 acgttatcag ccaggctccc ggcaaggtgg tgttgcgcaa tttcgagggc tttatcacca
      541 cctacaccga gccgctggat tatgacccgc acgagatcag ggatcaggaa gcgctcatcg
      601 cgccgcggcc ggagccgggg caggaaggcg tgctggccct gctggaaggg gagcggatgt
      661 cgatcgaacc gtcgggattc gcctccaccc atgcccgtgg agggggcgag catcgcctgc
      721 gcagcgggga tatctccggg aaatggcagc cgctgggcgt cggctcaatc gaagtccata
      781 agccccgcga gcggctcaac gaaggcaagg cccacgaagc ccacgaagct cacaacgaga
      841 aggaaacgga accgcccgca caaggctaga agcattggtg cacgccgcag cgcagacagg
      901 aatgtctgcg ctacggcggg tctcccgccg cccgaatctt gccgcccgcc agatgtttgg
      961 cggcgatgta gccgaacgtc atcgacgggc cgatggtgct gcccgcgccg gggtaggtgt
     1021 tgcccatgac cgaggctgaa ttattgccgg tcacgtagag gccctcgatg acggagccgt
     1081 cttcgcgtag tacgcgagcg tactcgtcca tcaccagccc gccctttgtg cccagatcgc
     1141 ccggcaccat ctccacggcg tagaacggcg gcttctcgat gggcgcgagg caggggttgg
     1201 gcttcaccgt cgggtcgccg tagtagcggt cataagcgct gtcgccgcga tggaactcct
     1261 cgtctacccc gctgcgggcg aaccggttga agcgttcgac cgtctcacgc agcccggccg
     1321 gatcgatgtc gcattgctcg gccagctctt ccagcgtgtc ggcccgtttg aaatagccgc
     1381 tctcgaagta agcatcgggc accggctgca tggggaacag cgtgccgaag atgtatttgc
     1441 ggcgatagcg gctgtccatg atgaaggtcg ccgggatgtg gcggttttcg atggtgtggt
     1501 tctcgtacat cgcgtggccg acttcgacat aggaagcggc ctcgttggtg aatcgcttgc
     1561 cggcggcatt gaccatgatc gcgccggggt aggagcgctc gccgacgtgg aacatcaccg
     1621 acgcgtcggg cggcaggctc gacggccccc accaggcgtc gtccatcagg tcgatcttcg
     1681 cgccgagctt tatgccca
//
LOCUS       DAOMQG010000556         1694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26794_length_1694_cov_5.067114, whole genome shotgun sequence.
ACCESSION   DAOMQG010000556 DAOMQG010000000
VERSION     DAOMQG010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26794_length_1694_cov_5.067114"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1694
                     /locus_tag="PLC06_17110"
     CDS             71..>1694
                     /locus_tag="PLC06_17110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRO92224.1"
                     /translation="MIDSKARVWPWLLLITLLAALIRLPYWDVIPAAFDEVDQTSYAY
                     QIAHGQIVPLTGNDAYSGPLYVYLVAGLIKLGATDPLVGRWIVLAAGVLTVPVVYAWV
                     LALGRKRLAALIAALFVALNPDLIFVNSHSGGTTLLLPFLTTLFLLCLTLAATRDSPA
                     WLLPTGVFAGLAMQSNMIAALAVAGGLLWFLWQARGKARLGRRWPLWPVVAGIIALLV
                     FSPVIIHAVIDDSGSAAGLETKPYIWEHDPTIATTINNTRRFWLQLGRQTSGVLIGDE
                     KFNALLGVPLLYLALMAAGLVHTTRRISTQPLLVIIPFALVMPVVSSHYGFGVIGRYT
                     MLLVSVWGAVIAFLLAAAIDRTWRLPAGGRRTLSIALLAIMALILIVWPVVSLFRYYR
                     QTDLAGETGRTVLDLSRYPVTHNQDEPVYLSTIEALAAVRGIPYVPHAAFLLGDVYHE
                     FLSPEEIIGRLYENPGPAYFLLNDGDAAVIGATIPIERVPIPANDAAAPRGFGLYRFA
                     GDQPLAKPEFVLDAAPAGLEPNALFGESVQLLG"
BASE COUNT          254 a          568 c          499 g          373 t
ORIGIN      
        1 cgcgcatcta tgacgggccg gatgaggtac acaagtggtc ggtggcgcgg cgtatcctga
       61 gccggtacgc atgatcgatt cgaaggcgcg cgtttggccc tggttgctgc tcatcacgct
      121 gctcgctgcg ctcattcggc tgccctattg ggacgtcatc ccggccgcct tcgatgaagt
      181 ggatcaaacc tcatacgcct atcaaatcgc ccacggacaa atagttccgc tgacgggcaa
      241 cgacgcctat tccgggccgc tctacgtcta tctggtcgcc gggctaatca agctgggagc
      301 tactgacccg ctggtcggcc gttggattgt cctggcggcc ggcgtcctga ctgttccggt
      361 tgtctacgcc tgggtgttgg cgctcggtcg gaagcgactc gccgcactta tcgccgcgct
      421 gttcgtggcc ctcaatcctg atctgatctt cgtcaatagc cacagcggcg gcacgacatt
      481 gctgttgccc ttcctgacga cgctctttct gctgtgcctt actcttgctg caacacggga
      541 tagccccgca tggctgctcc cgaccggggt gttcgccggg ctggcgatgc aatcaaacat
      601 gatcgccgcg ctggcagtcg ccgggggatt gctctggttc ctgtggcaag cgcgcggcaa
      661 ggcacgcctg ggccggcgct ggccgttgtg gccggtcgtc gccgggatca tcgccctgct
      721 cgtctttagc ccggtcatta tccatgccgt tatcgacgat tccggctcgg ccgccgggtt
      781 ggagacgaag ccttatatct gggagcacga cccgaccatc gcgaccacca tcaacaatac
      841 ccgacggttc tggctgcaac tggggcggca gacaagcggc gtgctgattg gtgacgagaa
      901 gttcaacgcg ctgctgggcg tccccttgct ctacctggcg ctcatggccg ccgggcttgt
      961 ccatacgact cgccggatat caacccagcc gctgctggtc atcatcccgt ttgcgctggt
     1021 gatgcccgtc gtcagcagcc attacggctt cggcgttatc ggccgctata cgatgctgct
     1081 cgtctccgtt tggggagcgg tcatcgcctt tttgctggcg gcggccatcg accggacatg
     1141 gcggttgccc gccggcgggc ggcgaacact ctcaatcgcc ctgctcgcga tcatggctct
     1201 cattctcatc gtctggcctg ttgtctcgct tttccgctac tatcggcaga ctgatcttgc
     1261 cggcgagacc ggtcgaaccg tcctggacct atcgcgttat cccgtcaccc acaatcagga
     1321 cgagccggtc tatctcagta ccatcgaggc gctcgccgcc gtgcgcggta tcccttacgt
     1381 cccccacgcg gcgtttctgt tgggtgatgt ttatcacgag ttcctctcgc ccgaagagat
     1441 catcggccga ctctatgaga accccggccc ggcctacttc ctgctcaacg acggtgacgc
     1501 ggccgtgatt ggtgcgacaa tccccattga gcgcgtgccc attcccgcca acgacgcggc
     1561 cgccccgcgc ggcttcggcc tctatcgatt cgcgggcgat cagcctcttg ccaagcctga
     1621 attcgtcctc gacgcagctc ccgccgggct ggaacccaac gcactcttcg gcgaaagcgt
     1681 ccagctgctg ggct
//
LOCUS       DAOMQG010000557         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26930_length_1687_cov_8.354167, whole genome shotgun sequence.
ACCESSION   DAOMQG010000557 DAOMQG010000000
VERSION     DAOMQG010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26930_length_1687_cov_8.354167"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLC06_17115"
     CDS             complement(<1..731)
                     /locus_tag="PLC06_17115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRO92225.1"
                     /translation="MNNLPLVSVIMPVRDEAAYIERSLGSVLAQDYPADRLEILVVDG
                     MSTDGTREVVVAAQATRSNLRLLDNPRGIVPPGLNIGIAEAQGDIVVRVDGHCEIATD
                     YVRRCVEHLLADDVEAVGGPIETIGETDEAQAIALAMSSWFGVGGSAFRTINDRPMLV
                     ETVAFPAYTRETLRRLGPFDEELVRNQDDEYNYRLLKSGGRILLSPDIRSRYYSRGSL
                     RKLWRQYYQYGFWKVRVMQKHPRQMR"
     gene            complement(808..>1687)
                     /locus_tag="PLC06_17120"
     CDS             complement(808..>1687)
                     /locus_tag="PLC06_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92226.1"
                     /translation="FSLWLAKQPNSEVPFVPVFYAALLLFWLALLRGRGGARSWALFL
                     VAGALTGAAMLIRPAAIGLSVIMALTILLFAVRGAALRTRLGYGALVVLGSLLVVLPW
                     EAAVYARTGKIIPLSSGGAATIHDGLTFLAVPKEYRREVNVPDDVADLMWAIHERRDE
                     TATTGGVFRVMAEEARAAPVAFGKLMLMKLARSWYGIDSRMLELPTLLIQGVYLIFIV
                     WGTIYCWRQGGALRRMIGGNWLIVGYFWGMTFLVVPLLRYMAPVMGLLMLALPGVYYS
                     FAAWRKERTVSRAASV"
BASE COUNT          371 a          583 c          472 g          261 t
ORIGIN      
        1 cgcatctgcc gcgggtgctt ctgcatgacc cgcaccttcc agaagccgta ctgataatac
       61 tgtcgccaca gcttgcgcag gctcccgcga ctatagtagc gcgagcgaat gtcgggcgac
      121 agcagaatgc ggccgccgct tttcagcaga cgatagttgt actcatcgtc ctgattgcgc
      181 accagctctt catcaaacgg gccgaggcgt cgcaatgtct cgcgcgtgta ggccgggaag
      241 gcgaccgtct caaccagcat cggccgatcg ttgatcgtgc ggaaggccga gccgcccacg
      301 ccaaaccacg aactcatagc cagggcgata gcctgcgcct cgtcggtctc accgatcgtc
      361 tcaatcggcc cgccgaccgc ttccacgtca tcagccagca gatgctccac acagcgccgc
      421 acgtaatcgg tcgcaatctc gcagtgaccg tcgacacgca cgacgatgtc tccctgcgcc
      481 tcggcgatgc cgatattgag acctggcggt acgatgcccc gcgggttatc aagcaatcgc
      541 agattggacc gcgtggcttg cgcggctacc acaacctcac gcgtgccgtc ggtcgacatg
      601 ccgtcaacga cgaggatctc cagccggtcg gccgggtaat cctgcgccag caccgatccg
      661 aggctgcgct cgatgtaggc cgcttcatcg cggacaggca tgatgacaga aaccagcggc
      721 aggttgttca tataacaagt gtacgcgcca agcgggccgc ccggcgtgga aaatgcgtga
      781 aggccttgtg ataaaatcag aaatcgctca aactgaggcg gcgcgcgaaa cagtgcgctc
      841 ttttcgccat gcggcgaagg agtaatagac gccgggcagc gccagcatca gcaaccccat
      901 gaccggggcc atgtagcgca acaggggcac aaccagaaac gtcattcccc agaaatagcc
      961 gacaataagc cagttgccgc cgatcatccg ccgcagcgct ccgccctgcc gccaacagta
     1021 gatcgtgccc cagacgatga atatgagata cacgccctga atcagcagcg tcggcaactc
     1081 cagcatacgg ctgtcgatac cgtaccagct ccgcgccagc ttcatgagca tcagcttgcc
     1141 gaacgcaacc ggcgcggcgc gtgcctcttc agccatgacc cgaaacacgc cgccggtcgt
     1201 cgccgtctcg tcccgccgct cgtggatggc ccacatcagg tcggccacat catcgggcac
     1261 gttaacctcg cgccggtact ccttggggac ggccagaaag gtgaggccat catggatcgt
     1321 cgccgccccg ccgctgctta gggggataat cttccccgtg cgtgcataga ccgccgcttc
     1381 ccagggcaac acaaccagca agctgcccag cacaaccaac gcgccatagc caagtctcgt
     1441 tcgcagtgcc gccccgcgga cggcaaataa caggatcgtg agcgccatta tgacgctcaa
     1501 cccaatcgcc gccggccgga tgagcatcgc cgcgccggtc aacgccccgg cgaccaggaa
     1561 gagcgcccag cttcgcgccc cgccgcgacc acgcaacaag gccagccaaa agagcaacag
     1621 cgccgcatag aacacaggca caaacggcac ctcgctattg ggctgcttgg ccagccacaa
     1681 cgaaaat
//
LOCUS       DAOMQG010000558         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_26951_length_1686_cov_11.022072, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000558 DAOMQG010000000
VERSION     DAOMQG010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26951_length_1686_cov_11.022072"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="PLC06_17125"
     CDS             complement(<1..1646)
                     /locus_tag="PLC06_17125"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HRO92227.1"
                     /translation="MVESFYISNVDNNTVNSIDAELMSIGENAYFWFDKGPGSVEPDL
                     AELARVTASFDEIYDSLYAYFGVSEPVGGRVHIVHAAPDVLCAIADKCHLAGYFSSRD
                     LLPQSVRPQSNERPMFVMNVQQFGTGNYLDVLAHELRHMLGSEYEAGEEDWFVEGGAM
                     FAEELAGFTFLPQLRGNLFLEDPDRQLNNWTDGDTIPYYGRGYLLNRFIYDRLGTGLY
                     REFILSGEPGLRGIDDVAQKHDLDLTGDQLWLDWLVAMALQDAKDVPTEYEWAGPTLN
                     GAAMTPINNLPANFETTVHQYAADYYELPSSGEVTIEFSGQPTVPLLNAAPPSGDRYW
                     YAQRANDSNPRLTRAIDLRAVAGATLEYDVFVDIEAGYDFAYVAVSADDGQTWQPLEA
                     EGMQGLRPEDDPSDSALAPRFYTGRSGAWVSKSIDLSEFAGQEILLRFEYVTDPILTF
                     AGFALDNIAIREIDFFDDAENLDEGWIAEGFTRVTSRLPQQWPLQLVTFDSDGRPTVE
                     RLTADGDGRLRHTFQALPGTRRPLLIVSAIAPETLELAHYS"
BASE COUNT          399 a          499 c          447 g          341 t
ORIGIN      
        1 gagtaatgag caagctccaa cgtctccggc gcaatggcgg acacaatgag caacggccgt
       61 cgcgttcccg gaagcgcctg gaatgtatga cgcaaccgcc catctccatc cgcagtgagt
      121 cgctcaacgg ttggtcggcc gtcactatcg aaagtgacga gttgcaaggg ccactgctgt
      181 ggcaatcgac ttgtaacgcg ggtgaacccc tcagcaatcc agccttcatc gagattctca
      241 gcgtcatcga agaagtcaat ctcgcggatg gcgatgttat ccagggcaaa acccgcaaac
      301 gttagaatcg gatcggtgac atactcgaac cgcagcaaaa tctcctgccc cgcgaactcc
      361 gagagatcaa tggatttgga gacccacgcg ccagatcggc cggtatagaa tcgaggggcc
      421 aaagcgctgt cggacggatc atcctccggt cgaaggcctt gcatcccttc ggcctcaagc
      481 ggttgccagg tttggccatc atcggccgac acggcgacat aggcgaaatc atatccagcc
      541 tcaatatcga cgaagacatc gtattccagc gtcgccccgg caacggcacg cagatcgatg
      601 gcgcgagtca agcgcgggtt gctgtcgttg gcgcgttgag cgtaccagta ccggtcaccg
      661 gaaggtggcg cagcgttcag aagaggtacg gtcggttggc cggaaaattc aatggtcacc
      721 tcaccgctcg atggtagttc ataataatcg gcggcatact gatgcacggt cgtttcaaag
      781 ttggccggga ggttattgat cggtgtcatc gccgcgccgt tcagcgttgg tccggcccat
      841 tcatattcag tcggaacgtc ctttgcatcc tggagcgcca ttgccaccag ccaatcgagc
      901 caaagctgat cgccggtgag atccaggtca tgcttttgag ccacatcgtc aatccctctc
      961 aagcctggct cgccgctaag gataaattcg cggtaaagac ccgtgcccag acggtcgtag
     1021 atgaaccgat taagcagata accccgccca taataaggaa tggtatcgcc atcagtccag
     1081 ttattcagct gcctgtccgg gtcctccagg aaaaggttgc cccgtaattg cggcaagaag
     1141 gtaaacccgg ccagctcctc agcgaacatc gcgccgccct cgacaaacca gtcttcctcc
     1201 cctgcctcat actcactccc caacatgtgg cgcagttcgt gtgccagcac atccaggtag
     1261 ttgcccgttc caaactgttg gacattcatg acaaacatcg gccgttcgtt cgactgtggc
     1321 cgcactgatt gaggaagcaa atcacgagat gagaagtaac cggccagatg gcatttgtcc
     1381 gcgatagcgc acaaaacatc aggcgcggcg tggacaatat gcacccgccc tcccacaggc
     1441 tcagatacac cgaagtaggc ataaagcgaa tcataaatct catcaaaact cgccgtcact
     1501 cgcgccagct ctgcgaggtc cggctcgacc gagcctgggc ctttgtcgaa ccagaagtag
     1561 gcgttttcgc cgatactcat caactctgcg tcgatagaat tgacggtgtt gttatctaca
     1621 ttgctgatgt aaaacgattc gaccattccg acctgatact caaccgatgg cctgggcgtc
     1681 gcctga
//
LOCUS       DAOMQG010000559         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_27034_length_1681_cov_9.823493, whole genome shotgun sequence.
ACCESSION   DAOMQG010000559 DAOMQG010000000
VERSION     DAOMQG010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27034_length_1681_cov_9.823493"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1666
                     /gene="gltX"
                     /locus_tag="PLC06_17130"
     CDS             176..1666
                     /gene="gltX"
                     /locus_tag="PLC06_17130"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRO92228.1"
                     /translation="MTVRVRFAPSPTGYLHIGGARTALYNWMFARKNGGQFILRIEDT
                     DQKRSVKGATQALIDALRWMGLDWDEGPGIGGPHGPYVQSERADLYRQWAQWLLDRDH
                     AYKCFCTADELKERRAAQKAARGDQGYDRRCRYLTPDELAARETAGTPFAVRFKAPLE
                     GATIIPDVIRGDITVPNAQIADAVLLKSDGLPTYHLANVVDDHFMEITHILRADEWIS
                     SAPLHINLYAAFGWEHPVYAHLPLVLNPSGKGKLSKRTQAFDDGGREVLVKVEEFRDA
                     GYLPQALNNFLANVGWSFGDDREKFPIEEAIPRFRLEDINPAPTRLPYDKLDWLNGQW
                     IQDMEPLELAQAVKPFLERQGHEISAEALLAVAPSLRVRLKKLTDAADFLKFLWEDEE
                     SAVPPAERLTHAKMPGDAALRGLQSARAFLALAQPFDTATLSAGLTAIGEENTTNGKA
                     GPFLGVLRLAVTQQDVSPPVFESIVALGRNRTLSRLDDAIEVLAKE"
BASE COUNT          328 a          590 c          481 g          282 t
ORIGIN      
        1 caactactgc caacttcccc cttttctgct atccttacgg tttacaaaca acctgtagct
       61 tgaagaagag gagtccacag attacgcaaa ttgggaaagg gaaaaaggga gatcgcccct
      121 tcaccccttc aaatctgtgg attctcttct tctgtgaaat ctgtggattc tctttatgac
      181 cgtacgtgta cgattcgccc ccagccccac cggctacctc cacatcggcg gggcgcgcac
      241 cgccctctat aactggatgt tcgcccgcaa aaacggcggc caattcatcc tgcgcatcga
      301 ggacaccgac cagaaacgct ccgtcaaggg cgcgacccag gccctcatcg acgccttgcg
      361 ctggatgggt ctggactggg acgaaggccc cggcatcggc ggcccccatg gtccctacgt
      421 ccagagcgag cgcgccgacc tctaccgcca gtgggcgcaa tggctgctgg accgcgacca
      481 cgcctacaaa tgcttttgca cggccgacga gctgaaagag cgtcgcgccg cccagaaagc
      541 cgctcgtggc gatcagggct atgaccggcg ctgccgctat ctgaccccgg acgagcttgc
      601 cgcccgcgaa acggccggga cgccctttgc cgtccgcttc aaggccccgc tggagggtgc
      661 gacgatcatc cccgatgtca tccgcggcga catcaccgtg cccaacgccc aaatcgctga
      721 cgccgtcctg ctcaagtccg acggcctgcc gacctaccat ctggccaacg tcgtcgacga
      781 ccacttcatg gaaataaccc acatcctgcg cgccgacgag tggatcagca gcgcgccgct
      841 gcatatcaac ctctacgccg ccttcggctg ggagcacccg gtctatgccc acctgccatt
      901 ggtgctgaac cccagcggca agggcaagct gagcaagcgc acgcaggcct tcgacgacgg
      961 cggccgcgag gtgctggtca aggtcgagga gtttcgcgac gccggctacc tgccccaggc
     1021 gctcaataac ttcctggcca acgtcggctg gtccttcggc gacgaccgcg agaagttccc
     1081 catcgaggaa gccattccgc gcttcaggct ggaagacatc aaccccgcac ccacgcgcct
     1141 gccgtatgac aagctcgact ggctcaacgg ccagtggatt caggacatgg agccgctgga
     1201 gctggcccag gcagtgaagc cgtttctgga gcggcagggg catgagatca gcgccgaggc
     1261 tctgctggcc gtggctccct cgctgcgcgt gcgcctgaag aagctgaccg acgccgccga
     1321 ctttctcaaa ttcctgtggg aggacgaaga atccgccgtg ccgccggccg aacgcctgac
     1381 gcatgccaaa atgccgggcg atgccgcact gcgtggcctg caatcggccc gcgccttcct
     1441 cgccctggcc cagcccttcg acacggccac cctgtcggcc gggctgacgg ctatcggcga
     1501 ggaaaacacc accaacggta aagccgggcc gttcctgggt gtcctgcgcc tggccgtcac
     1561 ccagcaggac gtctcgccgc cggtgttcga gtccatcgtc gccctcggcc gcaaccgcac
     1621 actgagtcgc ctcgacgacg caattgaggt actggcaaaa gaataagagc gtccacagat
     1681 t
//
LOCUS       DAOMQG010000560         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_27075_length_1679_cov_8.257389, whole genome shotgun sequence.
ACCESSION   DAOMQG010000560 DAOMQG010000000
VERSION     DAOMQG010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27075_length_1679_cov_8.257389"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1679)
                     /locus_tag="PLC06_17135"
     CDS             complement(<1..>1679)
                     /locus_tag="PLC06_17135"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRO92229.1"
                     /translation="PLTAGLIRDYLRDGAAGDNDLHAWQDWFLARRIDGRYSREQQLE
                     WAINTAYFGNLAYGVDAAARVYFGKGAADLTTAEAAILAAVAQSPVANPFDDPPAARQ
                     GQASILQTMVAAGMLSAAEAENALAEAPALAPSPGSDAIAPVFARLARRELEHILGPE
                     RLLAGGWQVETTLDLGLQEQLDCLSAASSGPADNTGGGPLCPARGFMLNAAADDLPAV
                     DDQPAMVALDPATGRLRAVAGDVIVPRSIGTLAQPLIYLTALSQGYNAATLTLDVPAI
                     YLQDGRPYSPRNADGDFFGPLRLREAMAAARTVPATQVLSWIGGMQVMTTARALGLEP
                     GTAAPDLTFPTVGFPVPLLDLARAYATVGSGGRMAGFAADGAIGEALPQPATIQRITA
                     ADGREFYTYEPTTRQTLSPELAYLLTDILSDAEARCPAGSCPDWPALPGGQPAAVVLA
                     AVVPAAGATGESTDGSWALGYTPDTLIGVMAAGDAADIWRGLMAWSVEGKPVAVWPRP
                     DSLRTAEVCAVSGLLPSRRADCPTVREWFVPGTEPFETDNMVREVAVNRETG"
BASE COUNT          277 a          580 c          564 g          258 t
ORIGIN      
        1 cccgtctcgc gattgacggc cacctcacgc accatgttgt ctgtctcaaa gggttccgtg
       61 ccgggcacga accactcgcg cacggtggga cagtcggccc gacgcgaggg tagtaatccc
      121 gacacagcgc acacctcggc cgtccgcaag ctatccgggc gaggccagac ggcgaccggc
      181 ttgccctcga ccgaccaggc catcagcccg cgccagatgt cggccgcgtc gccggcggcc
      241 atcacgccga tcaaggtgtc gggcgtgtag ccgagtgccc aggagccatc cgtcgattcc
      301 ccggtagccc cggcagccgg gaccacggcc gctaggacca cggccgccgg ttggccgccc
      361 ggcaacgccg gccaatcggg gcaactacct gccgggcagc gcgcctcggc gtcgctcagg
      421 atgtcggtca gcagatacgc cagttccggc gagagcgtct gccgcgtcgt tggttcgtag
      481 gtgtagaatt cgcgaccatc ggcggccgtg atgcgctgga tggtggccgg ttgcgggagc
      541 gcttcgccga tcgccccatc cgccgcaaac ccggccatcc ggccgccgct accgaccgta
      601 gcataggcgc gcgccagatc gagcagcggg accggaaacc cgacggtcgg gaaggtcaga
      661 tcgggcgcgg ctgtgcctgg ctccagaccc agcgcgcggg ctgttgtcat gacctgcatc
      721 ccgccgatcc agctcagcac ttgtgtcgcc gggacggttc gcgccgcggc catggcttcc
      781 cgcaggcgca aggggccgaa gaaatcgccg tcggcattgc gcggggaata agggcggccg
      841 tcctgcaaat agattgccgg tacgtccagc gtcagtgtgg ccgcgttgta accctggctc
      901 agcgcagtga gatagatgag cggttgggca agcgtgccga tggaacgggg cacgatcaca
      961 tcacccgcca ccgcgcgcag cctgccggtc gccgggtcga gggcgaccat cgccggttgg
     1021 tcatcgaccg ccggaagatc gtcggcggcc gcattcagca tgaacccccg cgccggacaa
     1081 agcggaccgc cgcccgtgtt atcggctggg ccactgcttg ccgccgacag acagtcgagt
     1141 tgttcttgca ggccgagatc aagggtcgtt tctacctgcc aaccgccggc cagcaggcgc
     1201 tccggcccca ggatatgttc cagttcgcgg cgagccaggc gggcgaaaac gggggcgatt
     1261 gcgtcgctgc ccggcgacgg ggccagagcc ggcgcttccg ccagagcgtt ttcggcttcc
     1321 gcggccgaaa gcatcccggc ggcgaccatc gtttgcagga tcgacgcctg tccctgtcgc
     1381 gccgccggtg ggtcatcgaa cggattggcg accgggcttt gagcaactgc ggccagtatc
     1441 gccgcctcgg ccgtcgtcaa gtcggccgcg cccttgccga aatacacccg cgccgcggca
     1501 tcgacgccat aagccagatt gccgaagtag gcggtgttga tcgcccactc cagctgctgt
     1561 tcgcgtgaat agcggccgtc aattcgacgg gccaggaacc agtcctgcca ggcgtgaagg
     1621 tcattgtcac cagccgctcc gtctctcaaa tagtcgcgga tcaacccggc agtgagcgg
//
LOCUS       DAOMQG010000561         1677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_27120_length_1677_cov_9.587546, whole genome shotgun sequence.
ACCESSION   DAOMQG010000561 DAOMQG010000000
VERSION     DAOMQG010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27120_length_1677_cov_9.587546"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1236
                     /gene="msrP"
                     /locus_tag="PLC06_17140"
     CDS             244..1236
                     /gene="msrP"
                     /locus_tag="PLC06_17140"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HRO92230.1"
                     /translation="MLIKKRTGIEIPSSEITPRNIYMSRRAFMAGSAAVAGAGLLAAC
                     APKTNLTGAGSAGRLAGPSAESATPAVIPGQTDELGDFLTPFNDVTGYNNYYEFSTDK
                     QAVAEKAQGFPTSPWTVAVSGLVGKPETYDIDALLHQFDQEERIYRLRCVEGWSMVIP
                     WQGFPLAALLKQVEPTAGAKFVRFETLYDPDDMPGQRSPFFRWPYVEGLRLDEAMNDL
                     TLMATGLYGEALLPQNGAPLRLVVPWKYGFKSIKSIVKIELTDEMPESLWMNAAANEY
                     GFYANVNPEVDHPRWSQATERRIGEGGRRPTLLFNGYGEQVASLYAGMDLRENF"
     gene            1373..>1677
                     /locus_tag="PLC06_17145"
     CDS             1373..>1677
                     /locus_tag="PLC06_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92231.1"
                     /translation="MTERMTTTEGRKALSRPLLSREAQRRLLRLAYHAVGLFPLAWLL
                     FDFWFGFLGPEPIRAMILRTGKAAIIMLTLSLAATPAGIIFGWKQATVVRRPLGLYA"
BASE COUNT          323 a          502 c          534 g          318 t
ORIGIN      
        1 atacgcgagc gacgggcggc cgtccgtgcg cctgtcgtaa attcagcgtg aagaaggcgt
       61 gaacgactgc gtagaaatac ctgcgtcgac acggccggct tccgcttcat gagcctgtga
      121 taattggatt atgagtttcg actcgctttt tattacactg atgcatgcaa agccacggct
      181 tggacgtaac aagcgtatgg acgggcatga gacccgccat ccttgctcaa ggagatgaac
      241 gccatgttga tcaagaaacg caccggaatc gagatcccgt cctcggagat cacgccccgg
      301 aacatctata tgtcgcgccg cgcgttcatg gccgggagcg cggccgtggc cggcgcgggt
      361 ttgctggccg cctgcgcgcc gaagaccaac ctgaccggcg caggcagtgc ggggcgcttg
      421 gccgggcctt cggcggaatc ggcgacaccc gccgtcatcc ccggccaaac cgacgagctg
      481 ggcgatttcc tgacgccttt caatgacgtc accggctata acaactacta cgagttctcg
      541 accgacaagc aggcggtcgc ggagaaagcc caagggttcc ccaccagccc gtggactgtg
      601 gccgttagcg ggttggtggg caaaccggaa acctatgata ttgacgctct cctgcaccag
      661 ttcgatcagg aagagcgcat ctatcgccta cgctgcgtcg agggctggtc gatggtcatt
      721 ccctggcaag gcttcccgct ggccgcgctg ttgaaacagg tcgagccgac ggccggcgcc
      781 aagtttgtgc gcttcgagac gctctatgac cccgacgaca tgcccggcca gagaagcccg
      841 ttctttcgct ggccctatgt ggaagggctg cggctggatg aggccatgaa cgatctgacg
      901 ctcatggcga ccggccttta tggcgaggcc ttgctgccgc aaaacggcgc gccgctccgg
      961 ctggtcgtgc cctggaagta tggcttcaag agcatcaagt ccatcgtcaa gatcgagctg
     1021 accgatgaga tgccggagtc gctgtggatg aacgccgcgg ccaacgagta tggcttctat
     1081 gccaacgtca acccggaagt ggatcatccg cgctggtcgc aggccaccga acgacgcatc
     1141 ggcgagggcg gccggcggcc gacgctgttg ttcaacggct atggcgaaca ggtggcttcg
     1201 ctgtatgcgg ggatggattt gcgggaaaac ttttagggga acgacgaact acgaacgacg
     1261 agtgacgcgc ggtcagtaac taccagacca ctgaccactg acctactgat tactgactac
     1321 cgcgactcct gtcgcgcgac cactggatgt tagagggcaa cggattggca cggtgactga
     1381 acggatgacg acgacggaag gaagaaaggc cctatcgcgg ccgctgctct cgcgcgaggc
     1441 gcagcggcgg ttgttgcggc tggcctatca tgctgtcggc ctgttccccc tggcatggct
     1501 gctcttcgac ttctggttcg gcttcctcgg cccggagccg attcgggcga tgatcctgcg
     1561 cacgggcaag gcggccatca tcatgctgac cctgtcattg gcagctaccc cggccgggat
     1621 cattttcggc tggaagcagg cgacggtcgt gcggcggccg ctgggcctct atgcctt
//
LOCUS       DAOMQG010000562         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_27380_length_1664_cov_6.546924, whole genome shotgun sequence.
ACCESSION   DAOMQG010000562 DAOMQG010000000
VERSION     DAOMQG010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27380_length_1664_cov_6.546924"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..506)
                     /locus_tag="PLC06_17150"
     CDS             complement(213..506)
                     /locus_tag="PLC06_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92232.1"
                     /translation="MPAPLARANAAELQRNLAELTGHAAAHVRPYGKHLLIQMHRDDS
                     VDTVARLTEVARDKYTAAFRSHTGRWEPLPGPAADLKQTAELVTTLLAPYLKP"
     gene            complement(472..1602)
                     /locus_tag="PLC06_17155"
     CDS             complement(472..1602)
                     /locus_tag="PLC06_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRO92233.1"
                     /translation="MSPGPRAPVVKLSSQERESLEALIRRSTAAQRDVERAKVALMAH
                     QGLATSSIASALGLSRPTVTVWRERLACFGLDGLQEAPRPGRPRRIAEVQRLQLLALA
                     CEPAEDRGRATPTLDELRDRAVEHGVVQDISRSHLQRILQAGDVRPHRVRQWLHSPDP
                     QFREKVNAICELYRRAPKGSVVLSIDEKTGIQAIERKHPDRAPRLGRVRRREFEYIRH
                     GTQALTAALDVHSGQVLARCTDRRTQDDLLGFMGDVAAAYPSGAVHVIWDNLNTHRAQ
                     AVWDDFNARHGNRFVFHFTPLHASWVNQIELLFGIYSRRVLRHASHTSIEHLRQRTET
                     FIAQRNANASPFKWTFAGFELQTGEPKRFIKHARTPGKSQRR"
BASE COUNT          262 a          539 c          557 g          306 t
ORIGIN      
        1 tgtaagcccg ccgtgcgcca agggcgcacg gttcgtccgt gtgtcggcgc aggttgactt
       61 tgcgccagcg aagtcaaggc gcacagcgcc gtgccggagc tacaaggctg ccgcgccgcg
      121 tgcagcgtgc ctgcaaggcg tcgagcgaag cgagccgacc cgcaagtcca tcgtccttgc
      181 gctagtacaa catcccggaa atagctttgc agttatggtt tgaggtaggg agccagtagg
      241 gtcgtaacga gttcagcggt ttgcttcaga tcggccgctg ggccgggcaa gggctcccag
      301 cggccggtgt ggctgcggaa tgctgcggtg tacttgtcgc gggcgacctc ggtcagccgc
      361 gcgacggtgt cgacgctgtc gtcgcggtgc atctggatca gcaggtgctt gccgtagggc
      421 cgcacgtgcg cggcggcgtg accggtgagc tcggcgaggt tgcgctgcag ttcagcggcg
      481 ttggctcttg ccaggggtgc gggcatgttt gatgaacctc ttgggttcgc cagtttgcag
      541 ctcaaagccg gcgaacgtcc acttgaacgg gctcgcattg gcgttgcgct gggcgatgaa
      601 ggtctcggta cgctggcgca aatgctcgat cgaagtatgg ctggcatggc gcagcacgcg
      661 acgcgaatag atgccgaaca gcaactcgat ctggttgacc cagctggcgt gcagtggcgt
      721 gaagtgaaag acgaagcggt tgccgtgccg ggcgttgaag tcatcccaca cggcttgcgc
      781 gcgatgcgtg ttcaggttgt cccagatgac gtgcaccgcg ccgctcggat acgccgccgc
      841 gacatccccc atgaagccca gcagatcgtc ttgcgtgcgc cgatccgtgc agcgcgccag
      901 cacctggccc gaatgcacat ccagcgctgc cgtcagcgcc tgcgtgccgt ggcggatgta
      961 ctcgaactcg cgccggcgca ctcgccctag ccgcggtgcg cgatccgggt gcttgcgctc
     1021 gatggcttga atgcccgtct tctcgtcgat gctcagcacc accgagccct tgggcgctcg
     1081 tcggtatagc tcgcagatcg cgttgacctt ctcgcgaaac tgcgggtccg ggctatgcag
     1141 ccactggcgc actcggtgcg gccgtacatc gccagcttgc aggatgcgct gcaggtgact
     1201 gcggctgatg tcctggacca cgccgtgctc gacggcccga tcacgcaact cgtccagtgt
     1261 cggcgtggcc cggccgcgat cttcggccgg ctcgcacgcc agggccagca gttgcagccg
     1321 ctggacctcg gcaatccgtc gcggccgacc ggggcgcggc gcctcctgca gtccgtcgag
     1381 tccaaagcac gccaagcgct cgcgccacac tgtcaccgtc ggccgcgaca ggcccaacgc
     1441 cgacgcgatg ctcgacgtcg ccaatccctg gtgcgccatc agcgccacct tggcgcgctc
     1501 gacatcgcgc tgcgccgccg tgctgcggcg gatcagcgcc tccagcgact cacgctcctg
     1561 actcgacagc ttgacgaccg gtgctcgcgg gcctggactc acgagcgagg agtctacatc
     1621 gatcgtcctt aactgtaaac atatttatgg gatgttgtac tagt
//
LOCUS       DAOMQG010000563         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_27758_length_1645_cov_9.747170, whole genome shotgun sequence.
ACCESSION   DAOMQG010000563 DAOMQG010000000
VERSION     DAOMQG010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27758_length_1645_cov_9.747170"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PLC06_17160"
     CDS             complement(<1..565)
                     /locus_tag="PLC06_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92234.1"
                     /translation="METYVLFIALALGAYAIASREQKQRIALLGGHLQNYRIEKLMEA
                     LTEGYMRALGEESPQRREQILELMESNEASLSSQFNRFAAEIATLDAATARVSRLPFS
                     IPFADRLFPGAMFDLRKALDIHARGIANVVQNADNRSPKDRAYVLSAEMLLMQHTCHW
                     FCKSKTVASARLMARHKTTHEQVVAAVS"
     gene            complement(629..1531)
                     /locus_tag="PLC06_17165"
     CDS             complement(629..1531)
                     /locus_tag="PLC06_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO92235.1"
                     /translation="MNRIVACLWFDDAAEDAAHFYTSIFRNATIGRIARYGEEGHEIH
                     GRPAGSVMTVEFELDGYTLLGLNGGPLFHLTPAISLFAMFETEADIDAAWAALGDGGT
                     VRMPLDAYDWSKRYGWLDDRYGVSWQLALGKRADIGQAIAPSLLFVGPQCGRAEEALE
                     HYTAVFPDSRVEGILRHDGAGPDAAGTVMHAQFQLGGETFMVMDSAHPHDFGFTEATS
                     LIVNCDTQDEIDHYWQALSAVPEAEQCGWLKDKFGVSWQITPKILPELLADPDPETSA
                     RVMRALLGMKKLNIAALKRAHGGE"
BASE COUNT          272 a          474 c          563 g          336 t
ORIGIN      
        1 gggagacggc tgcgacgacc tgttcgtggg tcgtcttgtg gcgggccatg aggcgggccg
       61 aggcgacggt ctttgatttg cagaaccagt gacaggtgtg ctgcatcagc agcatttcgg
      121 ccgacaggac gtacgctcga tccttgggtg agcggttgtc cgcgttctgg accacgttgg
      181 cgatgccgcg ggcgtggatg tcgagtgcct tgcgcaggtc gaacatcgct cccggaaaca
      241 ggcgatcggc aaaggggatc gagaagggca gccggctgac gcgcgcagtt gccgcgtcga
      301 gcgttgcaat ttcggccgca aaacgattga actggctgct cagtgaggct tcgttggact
      361 ccatcagctc caggatttgt tcgcgccttt gtggcgattc ttcgccgagc gcccgcatgt
      421 agccttcggt aagggcttcc atcagctttt cgatgcgata gttttgcaga tggccgccca
      481 gtagcgcgat gcgctgcttt tgctcccgtg aggcgatcgc ataggcgccc aaggcgagcg
      541 cgataaacag tacgtaggtt tccatggccg cattgtcctt tgcttcaggg ggggcggcaa
      601 tgggcttcat tcgttccggc aacagatgtc actcaccccc atgcgcccgc ttcaacgccg
      661 caatattcag cttcttcatc cccagcaacg cccgcatcac gcgcgccgag gtttccggat
      721 cggggtcagc tagcagttcg ggcaggatct tcggtgtgat ctgccatgac accccgaact
      781 tgtccttgag ccagccgcat tgttcggctt cgggcacggc ggagagggct tgccagtagt
      841 ggtcgatttc gtcctgggta tcgcagttga cgattaagga ggtggcttcg gtgaagccga
      901 agtcgtgcgg gtgggcgctg tccatgacca tgaaggtttc gccgccgagt tggaactggg
      961 cgtgcatgac ggtgccggcc gcgtcggggc cggcaccgtc gtggcgcagg atgccttcga
     1021 cgcgggagtc ggggaagacg gcggtgtagt gttcgagcgc ctcttcggcg cggccgcact
     1081 gcgggccgac gaacagcagc gacggggcga tggcctggcc gatgtcggcg cgcttgccga
     1141 gcgcgagttg ccacgagacg ccataccggt cgtcgagcca gccgtagcgt ttgctccagt
     1201 cgtaagcgtc gagcggcatc cgcaccgtgc caccgtcgcc gagcgcggcc caggcggcgt
     1261 cgatgtcggc ctcggtctcg aacatggcga agagcgagat tgcgggcgtc aagtggaaca
     1321 gcggaccgcc gttgaggccg agcagggtgt aaccgtccag ttcgaactcg acggtcataa
     1381 ccgatccggc ggggcggccg tggatctcgt ggccttcttc gccgtagcgc gcgatacggc
     1441 cgatggtggc gttgcggaag atcgaggtgt agaagtgcgc cgcgtcttcg gcggcatcgt
     1501 cgaaccatag gcaggcgacg attcggttca ttgtgggggt ctcctggggg gtgttctcgt
     1561 ccgggcttgt ttaacccatc cccaccccaa ccctcccctt gagcaactgt gtttgaagtc
     1621 aagcgaaatg aatcgattca tccca
//
LOCUS       DAOMQG010000564         1635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_27955_length_1635_cov_6.083544, whole genome shotgun sequence.
ACCESSION   DAOMQG010000564 DAOMQG010000000
VERSION     DAOMQG010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27955_length_1635_cov_6.083544"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..782
                     /locus_tag="PLC06_17170"
     CDS             114..782
                     /locus_tag="PLC06_17170"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRO92236.1"
                     /translation="MDAENTPQLVQTGPERFEAGLIYRYQMPSGPGPYPTAVMLHGRM
                     GSEDVMWVFRKTIPRPWLVVAPRAPLPEQGYFSWLPPPDKWPELAEFGPAVEALTRFL
                     RALPSLYNADPERVYLLGFSQGAAVSLATALTTPKLVRGMAGLVGFAPEVAPDQVAGK
                     LEGMPVFMAAGTQDHTVPRDQTQFAVDLLRRAGADLDYHEYDSGHKLPAAGISDLRAW
                     FGAR"
     gene            826..>1635
                     /locus_tag="PLC06_17175"
     CDS             826..>1635
                     /locus_tag="PLC06_17175"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRO92237.1"
                     /translation="MVATTPGRRSTFPDRLNRSLIGDGDARIGVVSRLPFLGPAALVA
                     AALLLPDRLWTTLLAGTAALIAIAFIWAWALARGLAAERRLLFGWVSVGDRMEEEFAL
                     FNRSALPALWVEIRDHSNLPGYRIGAVRSVGASDHVRWRQSSVCVRRGQYHLGPWEIL
                     SGDPFGLFRVSRRYEASQEIVIHPPIHTALPIPLPPGHVEGRSRSRERSPLATINAAT
                     VREYQTHDPYRRIHWPTTARRDSLYVRQFERDAAGDIRLVLDCRAAAQLGEG"
BASE COUNT          243 a          583 c          522 g          287 t
ORIGIN      
        1 caataaaata atttatattg acaaaattcc ggcaatatgt atactagtaa ttggctcccc
       61 ccccagaggt gagcagtagg acgcccgtct tactgccacc tcattttttt attatggacg
      121 cggaaaatac accccaactt gtgcaaaccg ggccggaacg attcgaggcc gggcttatct
      181 atcgctatca aatgccgtcg gggccaggcc cctacccgac cgcggtgatg ctccacggcc
      241 gcatgggcag cgaggacgtg atgtgggtgt ttcgcaagac aatccccagg ccgtggctgg
      301 tcgtggcccc gcgcgcgccg ctgcccgaac agggctattt ctcgtggctg ccgccgcccg
      361 acaagtggcc cgaactggcg gagtttggcc cggcggtcga ggcgctgacc cgcttcctgc
      421 gcgccctgcc ttcactctac aacgccgatc cggagcgcgt ctacctgctg ggcttcagcc
      481 agggcgcggc cgtctctctg gccaccgcgc tcaccacgcc gaagctggtg cggggcatgg
      541 ccgggctggt gggtttcgcg cccgaagtcg ccccggatca ggtcgcgggc aagctggagg
      601 ggatgccggt cttcatggct gccgggacgc aagaccacac cgtgccccgc gaccagaccc
      661 aattcgcggt cgatctgctg cgccgggcgg gggccgatct ggactatcac gaatacgaca
      721 gcggccacaa gctgcccgct gccggcatca gcgacctgcg cgcctggttc ggcgcgcgct
      781 gacgagcctc tggccctccg gcgcggctta tggcacacta gagccatggt cgccaccacc
      841 cccggtcgcc ggtcaacctt cccggatcgc ctcaatcgca gcctgatcgg agacggcgac
      901 gcgcgcatcg gcgtggtttc ccgcctccca tttctggggc cggcggcgct ggttgcggcc
      961 gccctgcttc tccccgaccg tctctggacg acgctgctgg ccggaacggc cgcgctcatc
     1021 gctatcgcct tcatctgggc ctgggcgctg gcgcgcggat tggcggccga gcgtcgattg
     1081 ctgttcggct gggtctccgt cggcgatcgg atggaggagg agttcgcgtt gttcaaccgc
     1141 tccgccctgc ccgccctgtg ggtggagatt cgcgaccact ccaacctgcc cggctatcgc
     1201 atcggcgcgg tgcgcagcgt cggggcgagc gatcacgttc gctggcggca atcgtcggtc
     1261 tgcgtccgtc gcggtcaata tcacctcggc ccctgggaga ttctcagcgg cgatccgttt
     1321 ggcctgttcc gcgtgtcacg ccgctacgag gcgtcccagg agatcgtcat ccacccgccc
     1381 atccacaccg ccctgcccat tccgctgccg ccggggcacg tcgaggggcg ctcgcgcagc
     1441 cgcgaacgct cgccgctcgc cacgatcaac gcggccacag tgcgcgaata ccagacccac
     1501 gacccgtatc gccggatcca ctggccgacg accgcccgcc gcgattccct ctacgtgcgc
     1561 cagttcgagc gggacgccgc gggcgacatc cggctggtgc tcgattgccg ggccgccgcg
     1621 caactgggcg agggg
//
LOCUS       DAOMQG010000565         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28380_length_1614_cov_8.319436, whole genome shotgun sequence.
ACCESSION   DAOMQG010000565 DAOMQG010000000
VERSION     DAOMQG010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28380_length_1614_cov_8.319436"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..644)
                     /locus_tag="PLC06_17180"
     CDS             complement(78..644)
                     /locus_tag="PLC06_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92238.1"
                     /translation="MTRQITLRVRPGETVRFPPLCVACGQPADERLTLQKRRGQITRR
                     VDVPLCAECARRLARRSGEQERLVRLSLPAAVGVALLLAVLGLLVLPFDLWWLRLLVG
                     LVAGLAGGAIIREALMRRATAAELPERRAVREAARIADFTWRDMTLAFADEEIAVQVM
                     ALNPDSLPEEQSSEENPEVDVVETPDDK"
     gene            963..>1614
                     /locus_tag="PLC06_17185"
     CDS             963..>1614
                     /locus_tag="PLC06_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92239.1"
                     /translation="MSDNPDTQPSPSPGTRSPIRGPRRYLTRQRPKATAEDLTDRKIR
                     RWSEFWATIILSLATLLTAWAGYEAGKWGSNSAALNTQAAMMSIEGTRLRSEAQERLL
                     IDLSLFTEWINATSSGNTQLADLYRARFRDGFRPAFDAWLATDPLTNPDAPDSPFEMP
                     DYARIEKAAAETLIGHAGELGIIAEEAGAIGDRYTLIVVILAGALLLAGVADRFEWA"
BASE COUNT          345 a          499 c          460 g          310 t
ORIGIN      
        1 cacgactact ttcatattcc aacctccatc agagttcgaa aatggttaaa tacatagttg
       61 tattgccgaa gcacaaatca tttgtcatcg ggcgtttcaa cgacatcgac ttccggattc
      121 tcttccgacg attgctcctc gggcagagag tccgggttca acgccatcac ctgtacggcg
      181 atctcctcgt cggcaaaagc cagcgtcatg tcgcgccagg taaagtcggc gatgcgagcc
      241 gcctcgcgca cggcgcggcg ctccggcaat tcggccgccg tcgcccgccg catcaacgcc
      301 tcgcgaatga tcgcgccgcc cgccagacct gctaccaacc cgaccaggag ccgcagccac
      361 cagaggtcga agggcaggac gagcagaccc aacacagcca acaacaacgc gactccgacg
      421 gccgcgggca ggctcagtcg aaccagccgc tcttgctcgc cggagcgccg cgccagccgg
      481 cgggcgcact cggcacaaag tggcacatcg actcgccgcg taatctggcc gcgtcttttt
      541 tgtaatgtca ggcgttcgtc ggccggttgc ccgcaggcga cgcacaatgg cggaaagcgc
      601 accgtttcgc cgggacgcac ccgaagcgtt atctgtcttg tcatattagt cggccgcatg
      661 ccgcgtgtca tgtgtcatgt gtcagtattt cgggtgacac ttggcatgta ctgaccccaa
      721 ctgaggcgat tcctgtcgcc cgcctgccgg tcacagacga tgaataacaa accgcagcgg
      781 ggcgatggcg agcatgacgg atattattcg caacgatcac cgggatcgat cgcactatcc
      841 gacaattata atgccgcagc gcgttatggg caatttgcac agaacttcac gttgcttatt
      901 ggcggatgtg acgataatct agccggatac gttctttttc gtcaagccac tatatcacga
      961 tcatgagcga taatcccgac actcaaccat cgccttcccc cggaacccgc tcccccatcc
     1021 gagggccgcg ccgatatctg acccgccaac ggccaaaggc aacggccgaa gatttgaccg
     1081 accgcaagat caggagatgg tcggagttct gggcgacaat cattttgtcg ctcgccacgc
     1141 tgctcacggc ctgggccggg tatgaggccg gcaagtgggg cagcaactcg gctgcgctga
     1201 atacgcaagc cgccatgatg agcatcgaag gaactcgact gagatccgaa gcccaggagc
     1261 ggcttctcat cgatctctct ctattcaccg agtggatcaa tgccacaagc agtggcaaca
     1321 cgcaactggc tgacctctat cgggcacgat tccgtgacgg attccggccg gcttttgatg
     1381 cgtggctggc gacagatccg ttgacgaatc ccgacgcgcc cgatagtccg tttgagatgc
     1441 ccgactatgc ccggatcgaa aaggcggccg ctgagacgtt gatcggtcat gcgggtgaat
     1501 tgggaattat cgccgaagag gccggcgcta taggcgacag atacaccctg atagtggtca
     1561 tcctggccgg agcgctgctg ctggcgggcg tggccgatcg gtttgaatgg gcag
//
LOCUS       DAOMQG010000566         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28663_length_1601_cov_6.967012, whole genome shotgun sequence.
ACCESSION   DAOMQG010000566 DAOMQG010000000
VERSION     DAOMQG010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28663_length_1601_cov_6.967012"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLC06_17190"
     CDS             <1..1074
                     /locus_tag="PLC06_17190"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRO92240.1"
                     /translation="DLDETDLEHRLSAKETGRSFDDGASADTPAATLKAGISQVKPLW
                     TFRCEDEIRTRPAAAKGIIFAGAYDNNLYAVTADKGEFLWKYPTGGGLAGSPVVHEDA
                     VVFGSSDNSLYSLQLRKGRLNWQFKAEGSIYSTPAVRFDHVFFGADDGFFYVVNAMRG
                     QLYWKTNVYSPVRSTPWVSEELVYFGTESGQILALELGAGKTKWQAKAQRAVTSSPKV
                     MDDIVYVGASDAHVYAMDASSGWPIWRFATRGPVISTPILFQGALYIGSADGNLYAID
                     ITNGRRLWAYPTNGQIVSAPTAWGNTVYFGSTDGSIYGVSARRGELQWRFDAGSPIIG
                     SPTVLNGVLYVGATDNCLYALPL"
     gene            1082..>1601
                     /locus_tag="PLC06_17195"
     CDS             1082..>1601
                     /locus_tag="PLC06_17195"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRO92241.1"
                     /translation="METRPTTLIESGYRCENGAERERNEDSCLIVSSQFGGHFTMPPV
                     GLFVVADGMGGERDGHVASNVATRVFGEQVLSNLYFPLLRERPEPRETAVLGALEQAV
                     FAAHDAVQQPYADHAQPGAAHTMSGGNGGTTLTAGLILGRRLFIAHVGDSRAYLLADG
                     ELRPLTEDHSLVR"
BASE COUNT          278 a          583 c          480 g          260 t
ORIGIN      
        1 gacctcgacg agaccgacct cgaacatcgc ctcagcgcca aggaaaccgg acgcagcttc
       61 gacgacggcg cgagtgccga cacgcctgcc gccacgctga aggcgggcat cagccaggtc
      121 aagccgctgt ggactttccg ctgcgaagac gagatccgca cccgtccggc ggcggccaag
      181 ggcatcatct tcgccggagc ctacgataac aatctctacg ccgtgacggc cgacaagggc
      241 gagttcctct ggaaataccc caccggcggc ggtctggccg gttcgccggt ggttcatgaa
      301 gacgcggtcg tcttcggctc gtcggacaac tccctctatt cgctccaact gcgcaagggg
      361 cggctcaact ggcagttcaa ggccgaaggg tcgatctact ccacgccggc cgtgcgcttc
      421 gatcacgtct tcttcggggc ggatgacggc ttcttctacg tcgtcaacgc catgcgcggt
      481 cagctctact ggaaaaccaa cgtctacagc ccggtgcgct cgaccccctg ggtgtccgag
      541 gagctggtct acttcggcac ggaaagcggg caaatcctgg ccctggaact gggcgcgggc
      601 aagaccaaat ggcaggccaa agcccaacgg gccgtcacct catcacccaa ggtcatggat
      661 gacatcgtct atgtcggtgc gtcggatgcc cacgtctacg ccatggacgc cagctccggc
      721 tggccgattt ggcgcttcgc cacgcgcggc cccgtcatct cgacgcccat cctcttccag
      781 ggtgcgctct acatcggctc ggccgacggc aatctctacg ccatcgacat caccaacggc
      841 cgccggctgt gggcctatcc gacgaacggc cagatcgttt ccgcccccac cgcctggggc
      901 aacacagtct acttcggctc caccgacggc agcatctacg gcgtcagcgc ccggcgcggc
      961 gaactgcaat ggcgcttcga cgccggcagc cccatcatcg gctcgccgac cgtcctcaac
     1021 ggcgtgctct atgtcggcgc aaccgacaac tgcctctacg cgctgcccct ctaacgtggc
     1081 tatggaaaca cgccccacaa cactgattga gtccggctac cgctgcgaga acggcgcgga
     1141 gcgcgagcgc aacgaagact cctgcctgat cgtgtcgtcg cagttcggcg ggcatttcac
     1201 catgccgccc gtcggcctgt tcgtcgtcgc cgacggcatg ggcggcgaac gcgacgggca
     1261 cgtggccagc aacgtggcga cgcgcgtctt cggcgagcaa gtcctgtcga acctgtattt
     1321 tccgctgctg cgcgaacggc ccgaaccacg cgaaacggcc gttctcggcg cgctggagca
     1381 ggccgtcttt gccgcccacg acgccgtgca gcagccctac gctgaccacg cgcagcctgg
     1441 cgcggcccac acgatgtccg gcggcaacgg cggcacgacc ttgacggccg ggctaatcct
     1501 gggccgccgc ctcttcatcg cccatgtcgg cgactcgcgc gcctacctgc tggccgacgg
     1561 cgaactgcgc ccgctgaccg aagaccattc cctggtgcgc c
//
LOCUS       DAOMQG010000567         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28664_length_1601_cov_6.551746, whole genome shotgun sequence.
ACCESSION   DAOMQG010000567 DAOMQG010000000
VERSION     DAOMQG010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28664_length_1601_cov_6.551746"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLC06_17200"
     CDS             <1..339
                     /locus_tag="PLC06_17200"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
                     /protein_id="HRO92242.1"
                     /translation="AAKGFDGDLFFNYLFLVEEMGELATDLVKIWSESKRRLVDGRGP
                     AESLEEAIEQNRPALRGELADLLAFILKLANYTGIDLEKAYVEKMRVNVGRTWPAERG
                     SLEKAHIDES"
     gene            329..1444
                     /gene="queA"
                     /locus_tag="PLC06_17205"
     CDS             329..1444
                     /gene="queA"
                     /locus_tag="PLC06_17205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRO92243.1"
                     /translation="MKVSDFDYDLPEALIAQYPMEPRDASRLLVLDTAGGAIEHRHFR
                     DLIDYLRPGDILVANDTRVIPARLFARKPTGGRVEILLLSKRSDGTWEALAGGRNVEP
                     GLALSLLDHAGETTDMAAEVEATGEGALRVLRFSAPVEDWIETLGYAPLPPYIHEALD
                     DPERYQTVYARPVGSAAAPTAGLHFTADLLIALRDKGVLFETVTLHVGLDTFKPVTAD
                     EVTDHVIHTEWAQLSSDAARRINEAHLAGGRIVAVGTTTARVLETAALRSAGITGSLR
                     TISARDAAGETTNMCPWKPVAAFEGPTDLFIHPGYRFRAVGVLITNFHLPRSSLLMLV
                     SAFAGRETILATYRAAIAEGYRFYSFGDAMLILPGET"
BASE COUNT          281 a          505 c          528 g          287 t
ORIGIN      
        1 gccgccaagg ggttcgacgg cgacctgttc ttcaactatc tgttcctggt ggaagagatg
       61 ggcgagttgg cgaccgatct ggtgaagatc tggtccgaga gcaagcggcg gctggtggac
      121 ggccgcggcc cggccgagtc gctggaggag gcgatcgagc agaaccgccc cgccctgcgc
      181 ggtgagctgg ccgacctgct ggccttcatc ctcaagctgg ccaactacac gggtatcgat
      241 ctggagaaag cctacgtgga gaagatgcgc gtcaacgtcg gccgcacctg gccggccgaa
      301 cgcggctctc tggagaaggc gcacatcgat gaaagttagc gacttcgatt acgatctgcc
      361 cgaagcgctc atcgcccaat acccgatgga accgcgcgac gcgagccgtc tgctcgtgct
      421 cgacacggcc ggtggcgcta tcgagcatcg ccacttccgc gacctgatcg attatctacg
      481 gccgggggac attctcgtgg ccaacgacac ccgtgtcatc ccggcgcggc tcttcgcccg
      541 caagccgacc ggcggccggg tggagatcct gttgctcagc aagcgatcag acggaacgtg
      601 ggaggcgctg gccggcggcc gaaatgtgga gccgggcctg gcgctgtcgt tactggacca
      661 tgccggggaa acgaccgaca tggcggccga ggtggaggcg acgggcgagg gggcgttgcg
      721 cgtcctgcga ttcagcgcgc cggttgagga ctggatcgag acgctcggct atgcacccct
      781 gccgccctac attcatgagg cgctcgacga cccggagcgc tatcagacgg tctacgcccg
      841 gccggtcggc tcggccgccg cgcccacggc cgggctgcat ttcacggccg atctgctcat
      901 cgccctgcgc gacaaagggg tgctgttcga gacggtcacg ctccatgtcg gcctcgatac
      961 cttcaagccg gtcacggccg atgaggtgac cgaccacgtc atccacaccg aatgggcgca
     1021 gttgtcgtcc gacgcggcgc ggcgcatcaa cgaggcacat ctggccggtg gccgcatcgt
     1081 ggccgtgggc acgacgacgg cgcgcgtgct ggagacggcc gccttgcggt cggcgggtat
     1141 caccggcagc ttgcggacga tcagcgcccg cgacgcagcc ggcgaaacga ccaatatgtg
     1201 cccctggaaa ccggtggccg ctttcgaggg gccgaccgat ctgttcatcc atcccggcta
     1261 tcgtttccgg gcggtagggg ttctcatcac caacttccac ctgccccgtt cgagtctgct
     1321 gatgctggtc tccgcttttg ccggacgaga gacgatcctc gccacctatc gcgccgccat
     1381 agccgaaggg tatcgtttct attcgtttgg ggacgcgatg ttgatcctgc cgggcgagac
     1441 atagcaagcc ggctgctaac cgcaatctaa cgggacggcg ctatactgat cgttgggttt
     1501 gtcatcagac tcaaacgact atacaatcgc ggtttggctc acggtggcca atcgcgatag
     1561 gggtttttgc aaatgccttt gtacacgtat cgatgcaagg a
//
LOCUS       DAOMQG010000568         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28686_length_1600_cov_6.834304, whole genome shotgun sequence.
ACCESSION   DAOMQG010000568 DAOMQG010000000
VERSION     DAOMQG010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28686_length_1600_cov_6.834304"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLC06_17210"
     CDS             <1..473
                     /locus_tag="PLC06_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92244.1"
                     /translation="LTATPSATPAATITPTPTATGSPTPVFSATLSIVPHTTTLRVGE
                     VLTVTADLEVAAGCQYPIFELTLGQAEGETPIFVHVAPPTGIITGPIAMPSAWTFRAT
                     QPGTATFAARTFGEKNCDGAWIWQYVSGVSGVVNVLPRPYTAYFPVVVNGVNPK"
     gene            470..1006
                     /locus_tag="PLC06_17215"
     CDS             470..1006
                     /locus_tag="PLC06_17215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRO92245.1"
                     /translation="MTNITIRRATPDDAEAIQRLFAFPRVLWGTLQVPYMSLESRRKR
                     LTDVAEGTYPLVAVVDGEVVGQLTLHTAKSSSPRRRHAGAFGMAVRDDFHRRGVGTAL
                     MAALVDLADNWLNLHRLELEVFVDNEWAVHLYQKFGFVIEGRLVDFAFRDGEYADVFV
                     MGRIRGRGNGDNNKEVAN"
     gene            1031..1282
                     /locus_tag="PLC06_17220"
     CDS             1031..1282
                     /locus_tag="PLC06_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92246.1"
                     /translation="MADTYETTAVFSDEKTLVLDERLPIAGGRVRITVEPLEQVEHPS
                     DFLTRLKAIHDELFASGFVPRTREQVDAELDQLRSEWNN"
     gene            1242..>1600
                     /locus_tag="PLC06_17225"
     CDS             1242..>1600
                     /locus_tag="PLC06_17225"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRO92247.1"
                     /translation="MLSWINFVANGITNSITTFLNQIIPNAAEIAPLAAGMFSQAYAF
                     AADGRDYVLRLNAFEEDFAKDEFAYTKFASPALPIPRMVARGRYDETRHYAVTERCPG
                     VTMSRIPDADLPPLLPDL"
BASE COUNT          289 a          540 c          474 g          297 t
ORIGIN      
        1 ccctgaccgc cacgccgtcg gccacacctg cggctacaat cacccccaca ccgacagcga
       61 ccgggtcgcc cacgccggtc ttctcggcca ccctgtcgat cgtgccccac accacgaccc
      121 tgcgcgtggg cgaggtgttg accgtgacgg ccgatctgga ggtcgccgcg ggctgccaat
      181 atccgatatt cgagctgacg ctggggcagg ccgagggcga gacgcccatc ttcgtgcatg
      241 tcgcgccccc gaccggcatc atcaccgggc caatcgccat gccgtcggcc tggaccttcc
      301 gggccacgca gccgggcacg gccaccttcg ccgcccgcac tttcggcgag aagaattgcg
      361 acggcgcgtg gatttggcag tatgtgagtg gggtgtcggg ggtggtgaac gtgctccccc
      421 ggccctatac cgcttatttt ccggtcgtag tcaacggggt gaacccaaaa tgacgaacat
      481 caccatccgc cgcgccaccc ccgacgacgc cgaggccatc cagcgtctct tcgccttccc
      541 acgcgtgctg tggggcacat tgcaggtgcc gtacatgtcg ctcgaatcgc gccgcaagcg
      601 gctgactgac gtggccgagg gcacctaccc gctggtggcc gtggtcgacg gtgaagtcgt
      661 cggccaactg acgctccaca ccgccaaatc cagctcgccg cgccgccgcc atgcgggggc
      721 gttcggcatg gccgtgcgcg acgacttcca ccgccgcggc gtgggcacgg cgctcatggc
      781 cgcgctggtc gatctggccg acaactggct caacctccac cgcctcgaac tggaggtgtt
      841 cgtcgacaac gagtgggccg tccacctcta ccaaaagttc ggcttcgtca tcgaggggcg
      901 gctggtcgat tttgcctttc gcgacgggga gtatgcggat gtgtttgtga tggggaggat
      961 tcgaggaaga ggaaatggcg acaataataa ggaagtagcc aattagccac gaatcgggaa
     1021 taattggatc atggctgata cgtatgaaac cactgctgta ttctcggacg agaaaaccct
     1081 cgtgctggat gagcgattgc ccatcgcggg tggcagggtt cggatcaccg tcgagcctct
     1141 ggagcaagta gagcacccat cggattttct cacccgcctg aaggcgatcc acgacgagtt
     1201 gttcgcgtcg ggttttgttc cccgcactcg cgaacaggtt gatgctgagc tggatcaact
     1261 tcgtagcgaa tggaataact aattcaatca caaccttcct caatcaaatc atcccaaacg
     1321 cggccgaaat cgcccccctc gccgcgggca tgttctccca ggcttatgcc ttcgccgccg
     1381 acggtcgtga ttacgtcctg cgcctcaatg ccttcgagga ggatttcgcc aaggacgaat
     1441 ttgcctacac gaagttcgct tccccggcgc tgcccatccc gcgcatggtt gcccgcggcc
     1501 gctatgacga gacgcgccat tatgccgtca ccgagcgttg ccccggcgtc accatgagcc
     1561 gcatccccga cgccgacctg ccgcccctgc tgcccgacct
//
LOCUS       DAOMQG010000569         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28836_length_1594_cov_7.226771, whole genome shotgun sequence.
ACCESSION   DAOMQG010000569 DAOMQG010000000
VERSION     DAOMQG010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28836_length_1594_cov_7.226771"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..547
                     /locus_tag="PLC06_17230"
     CDS             71..547
                     /locus_tag="PLC06_17230"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRO92248.1"
                     /translation="MTTTTDRPRIVMGERKRIALVAHDNKKDDLLEWAAHNLSILREA
                     TLFATGTTGRILSERLGLPVARMESGPLGGDMELGAEIVRGAIDVLIFFFDPLEAQPH
                     DPDIRALLRIAAVWNIPVATNRATADFVVSSPLMTGPYERLLTSYVDYRTRLEIKE"
     gene            579..>1594
                     /locus_tag="PLC06_17235"
     CDS             579..>1594
                     /locus_tag="PLC06_17235"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRO92249.1"
                     /translation="MDIQIVPIESDRLRTPPTSDYGFCQVFANRMFTQRYSPNKGWHD
                     AKVELYAPFQLDPATTSLHYAQMIFEGLKAYRRADGRINLFRPWENMRRFNNSARRMA
                     MAPVDEEEHLAAIVKLIDIEAGWVPQQSDASLYIRPAMIAADHTLGVKASASYLHFVI
                     ISPVGAYFAEGFNPVSVYITDQYVRAVRGGVGEAKTGGNYAASLLAGEDARQRGYSQV
                     LWLDAIERRYVEEVGAMNICFVYDGKRIVTPKLTGSILPGVTRKSVIELGRHLGYEVE
                     EGMIDVHEMLADIRSGRITEVFGCGTAAVIAPVGKFGFRDEEIVINDGLAGPVSHRLF
                     RQLV"
BASE COUNT          295 a          531 c          495 g          273 t
ORIGIN      
        1 gcggcggaac gacgtacaat agccatgggg caggcgggcg ctctattctc gaatgatccc
       61 ggagtttcgg atgaccacga ccaccgacag gccacgcatc gtcatgggcg aacgaaagcg
      121 catcgccctg gtggcccacg acaacaagaa agacgacctg ctcgaatggg cggcccacaa
      181 cctcagcatc ctgcgcgagg cgactctctt cgccaccggc accaccggcc gcatcctgag
      241 cgagcggctg ggcttgcccg tcgcccgcat ggagagcggc ccgctgggcg gcgacatgga
      301 gttaggggcg gagatcgtcc gtggcgcgat cgacgtgctg atcttctttt tcgacccgct
      361 ggaagcccaa ccccacgatc ctgatatccg cgccctgctg cgcatcgccg ccgtgtggaa
      421 catccccgtg gccaccaacc gggcgacggc cgatttcgtc gtctcgtccc cgctgatgac
      481 cggcccctat gagcggctgc tgacatccta tgtcgattac cggacccggc tcgagatcaa
      541 agagtgagcc ggcctgacca ctttgaagga gaccccgtat ggatattcaa attgttccca
      601 ttgaatccga ccgcctgcgc acgccgccga cgagcgacta cggcttttgc caggtgtttg
      661 ccaatcgcat gttcacccag cgctattcgc ccaacaaggg ctggcacgac gcgaaggtcg
      721 agctgtacgc gccattccag ctggacccgg ccaccacgtc actccattac gcccagatga
      781 tcttcgaagg cctgaaggcc tatcgacggg ccgacggccg catcaacctg ttccggccgt
      841 gggaaaacat gcgccgcttc aacaactccg cccggcgcat ggccatggcc ccggttgacg
      901 aagaggagca tctggcggcc atcgtgaagc tgatcgacat cgaggccggc tgggtgccgc
      961 agcagtccga cgcctcgctc tacattcggc cggcgatgat tgccgccgac cacaccctgg
     1021 gcgtgaaggc cagcgccagc tatctccatt tcgtcatcat cagcccggtg ggggcctatt
     1081 tcgccgaggg gttcaacccc gtgtcggttt acatcaccga ccaatacgtg cgggccgtgc
     1141 gcggcggcgt gggcgaggcc aagacgggcg gcaactacgc cgccagcctt ctcgcgggcg
     1201 aggatgcgcg ccaacgcggc tattcgcagg tgctgtggct cgacgccatt gagcggcgct
     1261 atgtggaaga agtgggggcg atgaacatct gcttcgtcta tgacggcaag cgcatcgtga
     1321 ccccgaagct gaccggcagc atcctgcccg gcgtcacccg caagtcggtc atcgaactcg
     1381 gccgccatct gggctatgag gtcgaagaag ggatgatcga tgtccacgag atgctggccg
     1441 acattcgcag cggccgcatc accgaggtgt tcggctgcgg cacggcggcg gtcatcgccc
     1501 ccgtcggcaa gtttggcttc cgcgacgagg agatcgtcat caacgacggc ctcgccgggc
     1561 ccgtcagtca tcggctgttc aggcagctgg tcga
//
LOCUS       DAOMQG010000570         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28883_length_1592_cov_8.962915, whole genome shotgun sequence.
ACCESSION   DAOMQG010000570 DAOMQG010000000
VERSION     DAOMQG010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28883_length_1592_cov_8.962915"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLC06_17240"
     CDS             complement(<1..257)
                     /locus_tag="PLC06_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme A pyrophosphatase"
                     /protein_id="HRO92250.1"
                     /translation="MSTSCTLDDVRAALSLPDFDGFGAQRQMMPAGRPVGRAGEGATA
                     PRLGGVLLLLYCVDEKLHLVLTRRPDYDGVHSGQISFPGGR"
     gene            complement(341..1075)
                     /locus_tag="PLC06_17245"
     CDS             complement(341..1075)
                     /locus_tag="PLC06_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRO92251.1"
                     /translation="MSERQKRILAVDDNAYTLRLVEFTLREAGFRVTTAISGEEAVEQ
                     IEASGLPDLAIVDLHMPGISGFEFAHRVHQYSDLPIIMLTAVNHEGTVVEGLEEHAED
                     YIVKPFSPGELVARTNRVLRRIGEFAYDLEPQTRIDDRLLIDFPNRTAIVEGKPEALT
                     PTETKLLYILMRKAGRTVPTDYILRRLWPMEPVFEDRLHVHLHRLRRKIEDRKDKTRP
                     RYILSERGAGYSFRDLSGQERANPQD"
     gene            complement(1216..>1592)
                     /locus_tag="PLC06_17250"
     CDS             complement(1216..>1592)
                     /locus_tag="PLC06_17250"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HRO92252.1"
                     /translation="PGAMVRAMTAQFGSDPADLVAAVGPCIGPCCYEVGEEVIAAVEA
                     TFTEPDTLFRPLTGDDRLTAAGHRFFDMPQANQRNLTNAGVRNIETSGLCTACRTDLF
                     FSHRAEKGRTGRFGTVFALGRD"
BASE COUNT          292 a          480 c          531 g          289 t
ORIGIN      
        1 tggcggccgc cgggaaaaga gatttggccg gaatggacgc cgtcgtagtc cggccgccgg
       61 gtgaggacca ggtgcagctt ttcgtcaacg cagtagagca gcaacagcac cccgccaagg
      121 cgcggcgcgg tcgccccctc tccggcgcgg ccgaccggtc gcccggcggg catcatctgc
      181 cgttgcgcgc cgaagccgtc gaagtcgggc aggcttagcg ccgcgcgcac gtcgtcgagg
      241 gtgcaagagg tactcatagt gcgagtcgat taaacgagga tggcaaaggg tttcactgac
      301 gggagaggga gttcgaatga gaacgagcga accggcctgc ctaatcctgt ggattcgccc
      361 tctcttgccc gcttaggtcg cggaagctat agcccgcgcc acgctcggag aggatgtagc
      421 gcggccgcgt cttgtccttg cgatcctcga ttttgcgccg cagccggtgg agatggacgt
      481 gcagccggtc ctcgaagacc ggctccatcg gccacaggcg gcgcaggatg tagtcggtcg
      541 gcacggtgcg gcccgccttg cgcatcagga tatagagcag cttcgtctcc gtcggcgtca
      601 gcgcttcggg cttgccctcg acgatggccg tgcggttggg gaaatcgatc agcagccggt
      661 cgtcgatgcg cgtctgcggc tccagatcat aggcgaactc gccgatgcga cgcaggacgc
      721 gattggtgcg ggccaccagt tcgccggggc tgaagggctt gacgatataa tcctcggcgt
      781 gttcttccaa cccctcgacg accgtgccct catgattgac ggccgtcagc atgatgatcg
      841 gcaggtcgct gtactgatgg acgcgatggg cgaactcgaa accgctgatg cccggcatgt
      901 gcagatcgac gatagccaga tcaggcaggc cgctcgcctc gatctgctcg acagcctcct
      961 cgccggagat ggctgtggtg acgcggaacc ccgcttcgcg cagggtaaac tccaccagtc
     1021 gcaaggtgta ggcgttgtcg tcaaccgcca ggattcgttt ttgtcgttcg ctcatgaggg
     1081 gtgatttaac cacgtatacg cgcgaatggc acggattcca aagggaatgg taagccttgc
     1141 cggagagacc tgacaggtgg gcgtacgaga atctgcgcag gaatggaatt ttggtcaccc
     1201 acctgtcagg tcttattagt cgcggccgag tgcaaagacc gtcccaaacc gccccgtgcg
     1261 ccccttttcg gcgcggtggg agaagaaaag gtcggtgcgg caggcggtgc aaagaccgga
     1321 cgtctcgatg ttgcggacgc cggcattggt cagattcctt tgattggctt gcggcatatc
     1381 gaaaaagcga tggcccgcgg ctgtcaatcg atcatcgccg gtcagcggcc ggaacaaggt
     1441 gtccggctcc gtgaacgtcg cttcaaccgc ggcgatcacc tcttcgccga cttcgtagca
     1501 gcaagggccg atgcacggcc cgacggcggc gacgagatcg gccggatcgc tgccgaactg
     1561 cgcggtcatg gctcgcacca tcgcccccgg ca
//
LOCUS       DAOMQG010000571         1590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_28918_length_1590_cov_9.452117, whole genome shotgun sequence.
ACCESSION   DAOMQG010000571 DAOMQG010000000
VERSION     DAOMQG010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28918_length_1590_cov_9.452117"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..315
                     /locus_tag="PLC06_17255"
     CDS             58..315
                     /locus_tag="PLC06_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92253.1"
                     /translation="MQALKQWVLRHPNLAAWIALAVGMNGILLFEARDVGLLPMQWFW
                     LILITTLVAGACIWIVSWGDDEETTPDTAELKSSDDARSGS"
     gene            332..1459
                     /locus_tag="PLC06_17260"
     CDS             332..1459
                     /locus_tag="PLC06_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRO92254.1"
                     /translation="MDEFQPQVAPEQYEYVDAMRRAIADLFIIFDTTLDYPDKGYVRF
                     RGRFLQDPADCYDELRRRFEQYGYTPTVSKERDMVSLIALPVVFDHPKSRRSINLILF
                     IATILSTLFVGASYGAETTEQLWQIWRGWPFATSILLILGAHELGHYFAARHHNVPVT
                     LPYFIPMPLSLFGTLGAFIRLQGPVSTRRALFDVGAAGPLAGLFFALPILFFGLATSD
                     VGPIMPGGIYEGNSILYALAKLAVFGRLLPGNGLDVHLNQVAWAGWVGLFVTGLNLIP
                     VGQLDGGHVAYALLGDRAKGLFWPSIVGLALIVVYSWLQGAFTGTWLLWIFLLLSFGR
                     VHARPLEDISELDPRRRVLAIITLGLFFLTFVPFPLSGIVG"
     gene            1516..1587
                     /locus_tag="PLC06_17265"
     tRNA            1516..1587
                     /locus_tag="PLC06_17265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1548..1550,aa:Met,seq:cat)
BASE COUNT          248 a          466 c          508 g          368 t
ORIGIN      
        1 attcgtaatt cctaattttc tgtgcattct tcccggaagt cgctatagta gattcccatg
       61 caagcattaa aacagtgggt tctccgccat ccaaacctcg ccgcgtggat cgctctcgcc
      121 gtgggcatga acggcatcct gctctttgag gcgcgggatg tggggctgct gccgatgcag
      181 tggttctggc tcatcctcat caccacgctg gtcgccggcg cgtgtatctg gatcgtcagc
      241 tggggagacg acgaagagac cacgcccgac acggccgaac tgaaatcgtc cgacgacgcc
      301 cgctccggct cctgaggccc gatcgcaccc catggacgag ttccagccgc aggttgcgcc
      361 tgagcagtac gagtacgtcg acgccatgcg gcgggccatc gccgatctgt tcatcatttt
      421 cgacacgacg ctcgattatc ccgataaggg gtatgtgcgt tttcgcggcc gatttctgca
      481 ggacccagcc gactgttacg acgagctgcg gcggcgcttc gagcagtacg gctacacacc
      541 gactgtgagc aaggagaggg acatggtctc cctcatcgcc ttgccggtgg tgttcgacca
      601 tcccaaatcg cgccgaagta tcaacctgat cctgttcatc gccaccatcc tgtcgacctt
      661 gttcgtaggg gcgtcctatg gagcggagac aaccgagcag ctatggcaga tctggcgcgg
      721 ctggcccttc gcgaccagca ttctgctgat cctcggggcg catgagctgg gccattattt
      781 cgccgcccgt caccacaatg tgccggtgac gctgccttat ttcatcccta tgcccctgtc
      841 gctgtttggc acgctggggg cgttcattcg tttgcagggg ccggtcagca cgcggcgggc
      901 gctgttcgac gtgggcgcgg ccgggccact ggccgggctg tttttcgcgc tgcccatcct
      961 gttcttcggg ctggcgacgt cggacgtggg gccgatcatg cccggcggta tctatgaggg
     1021 taactccatc ctctacgcgc tggccaaact ggcggtgttc ggccgtctgt tgccgggcaa
     1081 cgggctggat gtgcatctga atcaggtggc ctgggcgggc tgggtcggcc tgtttgtgac
     1141 cgggctgaat ctgatcccgg tgggacaact ggacggcggc cacgtggcct acgcgctgct
     1201 gggggatcgg gccaaggggt tgttttggcc gtcgatcgtg gggttggcgc tcatcgtcgt
     1261 ctattcctgg ttgcaggggg ctttcacggg gacgtggctg ttgtggatct tcttgctgct
     1321 gtcgttcggc cgcgttcatg cccggccgct ggaagatatc tccgagctgg acccgcggcg
     1381 gcgcgtgctg gcgatcatca cgttgggtct cttttttctg accttcgtgc cgtttccgtt
     1441 gtcggggatt gtggggtagt taacatcccc tcatttggca aacggcgagg ggagcgctat
     1501 aattccgggg aaattgacgc catagctcag tggtagagcg ggggactcat aagccctagg
     1561 tcgatggttc aaatccatct ggcgtcacaa
//
LOCUS       DAOMQG010000572         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29034_length_1585_cov_4.949020, whole genome shotgun sequence.
ACCESSION   DAOMQG010000572 DAOMQG010000000
VERSION     DAOMQG010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29034_length_1585_cov_4.949020"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /gene="ssb"
                     /locus_tag="PLC06_17270"
     CDS             <1..410
                     /gene="ssb"
                     /locus_tag="PLC06_17270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRO92255.1"
                     /translation="RLGRDPEMRYMPDGTAVTSFSVATDRRWTDRATGQPVQETTWFR
                     VSVWGRQAEASNQYLSKGRMVLVEGRLTPDANGGPRIWMAQDGTARASFEIKADNVLF
                     LGGREDGGGSSSFDDSSQGGGKPAAHEEDDIPF"
     gene            complement(499..630)
                     /locus_tag="PLC06_17275"
     CDS             complement(499..630)
                     /locus_tag="PLC06_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92256.1"
                     /translation="MRAAGFTDISIVDKGEPAVELAGHPLGSGPARLFSARVTAVKP"
     gene            complement(698..1042)
                     /locus_tag="PLC06_17280"
     CDS             complement(698..1042)
                     /locus_tag="PLC06_17280"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRO92257.1"
                     /translation="MSAEPDESVSFAKAIADETRQRVMGLLCCRWLCVGDIVEEMQGV
                     TQPTVSHHLAVLREAGLVHVRREGKQVFYSLNQDEVVDCCGRIMRVFAPERVDASATV
                     DLSAIEVREARA"
     gene            1172..>1585
                     /locus_tag="PLC06_17285"
     CDS             1172..>1585
                     /locus_tag="PLC06_17285"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HRO92258.1"
                     /translation="MLATTAQQRVAIIDLGSNTARLVVMAAVPGYAWRLEDEIREVVR
                     LRQGMTAAGLSDEAMARALVTLRLFKRFCDSTQVDLIIATATSAVRDARNGAEFVRQV
                     ESDLGLPLRILDGEREAYYGVLGALNEVPLNDGYVL"
BASE COUNT          319 a          484 c          504 g          278 t
ORIGIN      
        1 ggcgtcttgg ccgggacccg gaaatgcgat atatgcccga tgggacggcc gtgaccagct
       61 ttagtgtggc gacggatcgg cgctggacgg atcgcgcaac agggcagcct gtgcaagaaa
      121 ccacctggtt ccgggtgagt gtttggggcc gccaggcaga agcctccaat caatacctga
      181 gcaagggcag aatggtgctc gtcgaaggtc gtttaacgcc ggacgcaaat ggcggcccgc
      241 gcatttggat ggcgcaagac ggaaccgcgc gcgcgtcatt tgagataaag gctgataacg
      301 tgttattctt gggcggtcgt gaggacggtg gtggctccag cagcttcgac gacagttcgc
      361 aaggcggcgg caaacccgcg gctcacgaag aagacgacat cccgttctaa agtacgaaac
      421 taaacagagg gggatgcaac agacaacttg ttgcccccct ctttcggtat tgccggtacg
      481 cgggttaaga accccttgtt acggcttgac ggccgtcaca cgcgcgctaa acagccgtgc
      541 cgggccgctc cccagcgggt gccccgccaa ttcgacggcc ggttcccctt tatcgacgat
      601 cgagatatcc gtgaaaccgg ctgctcgcat gaccccggca tagagcgcta cgtcctccgc
      661 gccggtgacg caaccggccc acgccgacag gttgagccta tgcgcgcgcc tcgcgcacct
      721 cgatcgctga cagatcgacg gtagcggatg cgtccactcg ctccggcgcg aaaacgcgca
      781 tgatacggcc gcagcaatcg acgacttcat cctgattgag cgagtagaag acctgcttgc
      841 cctcacggcg cacgtggacc agcccggcct cgcgcaggac ggccaggtgg tgggagacgg
      901 tcggctgggt gacgccctgc atctcctcaa cgatatcgcc cacgcacagc cagcgacagc
      961 acagcagacc catcacgcgc tgtcgcgttt cgtcggctat cgccttggca aaggaaacgg
     1021 attcatcggg ttcagcagac atatcgatat ccatcaatgt gaatattata ctggggatgg
     1081 aggggctgtc aagagggcgg acgacgcgac tcctgtcgcg cgacgggagt cgcggtggcc
     1141 tgtggtcggc cgtcggcgta taatgcaaac catgctcgct accactgcgc aacaacgtgt
     1201 cgccatcatc gacctgggtt ccaacacggc ccggttggtc gtcatggccg ccgtgccggg
     1261 ctatgcctgg cgtctcgaag atgagattcg cgaggtggtc cgcctgaggc aaggcatgac
     1321 ggccgccgga ttgagcgacg aagcgatggc ccgcgctctg gtgacattgc gactgttcaa
     1381 gcgcttctgt gacagcaccc aggtcgatct gatcatcgcc acggccacca gcgccgtccg
     1441 tgacgcccgc aatggcgcgg aattcgtgcg acaggtggaa tcggacctgg gactccccct
     1501 gcgcatcctg gacggcgaaa gagaagccta ttacggcgtg ctgggcgcgc tgaacgaagt
     1561 cccgctgaat gacggctacg tgctc
//
LOCUS       DAOMQG010000573         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29095_length_1582_cov_13.956778, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000573 DAOMQG010000000
VERSION     DAOMQG010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29095_length_1582_cov_13.956778"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLC06_17290"
     CDS             complement(<1..404)
                     /locus_tag="PLC06_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRO92259.1"
                     /translation="MNQVTYIGHATVLIEIDGVRLLTDPLLTNRVSHLLRTSAVSDVD
                     APHLDAVLLSHLHADHLHLRSMRRLGKEQRIIAPRGSAAWLARHGFRRVTEIGAGEVV
                     RVAGIDIEATPANHPSRTLPGRPRTACLGYLI"
     gene            complement(492..1058)
                     /locus_tag="PLC06_17295"
     CDS             complement(492..1058)
                     /locus_tag="PLC06_17295"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRO92260.1"
                     /translation="MQGYLAALSLLLLMSMVIARALWLHRSGIRVMNFGRIDKSDFLI
                     PPFALFYFYTVFAAAFGWPLLSDRRFFHSDLLAWVGVALCYAGLLFLLLSLIAFGKSF
                     RVGIDIDRADRLVTSGVFGITRNPIYVAFFGVLIGQFLVFPNWVTLAYLIGGIGLIHR
                     QVLREEEFLRHHYGQEYADYSRRVRRYL"
     gene            1185..1544
                     /locus_tag="PLC06_17300"
     CDS             1185..1544
                     /locus_tag="PLC06_17300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRO92261.1"
                     /translation="MLNLNSVMIGTKQPEVLAAYYEKVLGQPAGMVDEEYGFYGRQVG
                     SAFLSILDHSEMGGRTKDPGRIMFNFETAQVKEEFERIKAAGGTVIRNPYEMEGGWIA
                     TLADPDGNYFQLVTPMG"
BASE COUNT          383 a          422 c          498 g          279 t
ORIGIN      
        1 tggataagat agcccaggca cgccgttcgc gggcggccgg gcagcgtccg cgacggatga
       61 ttggccggtg tggcctcgat gtcgatgccg gccacccgga cgacttcgcc cgcgccgatc
      121 tccgtcaccc ggcgaaaccc gtgacgcgcc agccaggcgg ccgacccgcg cggggcgatg
      181 atccgctgct ccttgcccag ccgccgcatg gagcgcagat gcagatggtc ggcgtggaga
      241 tgggagagca ggacggcgtc gaggtggggg gcatcgacgt cggacacggc tgaggtgcgc
      301 agcaggtgac tgacgcggtt ggtcagcagc ggatcagtca acaggcgcac gccgtcgatc
      361 tcgatgagga cggtggcgtg accgatgtag gtaacttgat tcatggcggt aattatggca
      421 accggacgga tgagcggcaa aggatcggat gttgctctca taacacgggc cgggacgatc
      481 ccgggcctcg ttcacagata gcgtcggacg cgccgggaat agtcggcgta ttcctgaccg
      541 taatggtgtc gcaggaattc ttcctcgcgc aaaacctggc ggtggattag cccgatcccg
      601 ccgatgaggt aggccaatgt gacccagttg ggaaacacca gaaactggcc gatcagcacg
      661 ccgaagaagg ccacgtaaat cggattgcgc gtgatcccga agacgcccga cgtcaccagc
      721 cggtcggcgc ggtcgatgtc gatgccgacg cgaaagctct tgccgaacgc gatgaggctg
      781 agcagcagga acagcagacc ggcatagcag agagccacgc ccacccaggc cagaagatcg
      841 gagtggaaga agcgccggtc gctcagcagc ggccaaccga aagcggcggc gaagacggtg
      901 tagaaataga acaacgcgaa cgggggtatc aggaagtcgc tcttgtcgat tcggccgaaa
      961 ttcatgacgc gaatccccga tcggtggagc cacagggcgc gggcgatcac catactcatg
     1021 agcaaaagaa gcgacagggc ggccagatat ccttgcattg attagcctct ccttcgtcct
     1081 tcagatatag aatccataat aaaacgtcca aagaaacgac aatacccatg aaatagtttc
     1141 atgcggaaac gtatcaacct tatcctatcc aaggagtatt caaaatgcta aacctgaact
     1201 ctgtcatgat tggaacgaag caacccgaag ttctggccgc ttattatgaa aaagtcctgg
     1261 ggcaacccgc cggaatggtt gatgaggaat acggtttcta tggccggcaa gtggggtcag
     1321 cttttctgtc cattctggat cactccgaaa tgggtggtcg aacgaaggac cccggccgca
     1381 tcatgtttaa ttttgaaacc gctcaggtta aagaagagtt tgagcgaatt aaggccgcgg
     1441 gaggcacagt catccggaac ccttatgaaa tggagggggg atggatcgcg actctagccg
     1501 acccggacgg caattacttt caactggtaa ctccgatggg gtaaggttgc gcatgccgga
     1561 ttggcgcaag agattatcaa at
//
LOCUS       DAOMQG010000574         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29246_length_1576_cov_3.005917, whole genome shotgun sequence.
ACCESSION   DAOMQG010000574 DAOMQG010000000
VERSION     DAOMQG010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29246_length_1576_cov_3.005917"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLC06_17305"
     CDS             complement(<1..937)
                     /locus_tag="PLC06_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRO92262.1"
                     /translation="MATSNKNDKTSTTRQTSAKRQASATSKAKSNSHSKPAAKKPAAK
                     KSTSGKKAPPPRLDDGQRSLLAGIVILFLTAILTLSLLSPNQGQFTQWLSGLAGLAFG
                     IGRVVIPLITGGIGIFLVLRGMEQEPELPWFRLLGAAVVFLVLLALASMVAHQREQGT
                     YNSLYAVAAAGKGGGYLGSLLATALARLLGDLGTIATLMVVGLAGVVLAAGLSREQVA
                     GWFEREPRPSTGAEPAERAVRINPGRETTANATPAARPPAADRRPEPQLRLIPEEGEA
                     PARGMTAPAHDKPKRGKKEAPPPVFIEADRPATAVA"
     gene            1258..>1576
                     /locus_tag="PLC06_17310"
     CDS             1258..>1576
                     /locus_tag="PLC06_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92263.1"
                     /translation="MKQSRLIVLIALAFLAACRPATGGVTSTVVPATAVPTANVPTPA
                     PATATDEPSAPPATDGASTTAPLFDIDPFDRSLFAAGLISDEAGGDALPGAPVYHMAI
                     TIDP"
BASE COUNT          271 a          535 c          489 g          281 t
ORIGIN      
        1 gggcgacggc cgtcgcgggc cggtcggcct ctataaaaac gggcggcggt gcttctttct
       61 tgccccgctt gggcttatcg tgggcaggcg cggtcatacc acgcgccggg gcttcccctt
      121 cttcgggaat aagccgcaat tgtggctcag gccgccggtc ggcagccggc ggtcgcgcgg
      181 caggggtcgc gttggcggtc gtctcccgtc ccggattgat ccgcacagct cgctcggccg
      241 gctccgcgcc ggtcgacggc cgcggctcgc gctcgaacca gccggccacc tgctcgcgcg
      301 agaggcccgc ggccaacacg accccagcca gcccgacgac catcagcgtg gcgatggtgc
      361 ccaggtcgcc cagcagccgg gccagcgccg tggccagcag cgagccgaga tagccgcccc
      421 ccttgccggc cgcggccaca gcgtagagac tattgtaggt cccttgctcg cgctggtgag
      481 cgaccatcga ggcgagagcc agcaagacga ggaagacgac ggccgcgccc agcagtcgaa
      541 accagggcaa ctccggctcc tgctccatgc cacgcaggac gaggaatatg ccgatgccgc
      601 cggtgatgag cgggatgacg acgcggccga tgccgaacgc cagcccggcc aaccccgaca
      661 gccattgagt gaactgcccc tgattgggcg agagcaggct gagggtcaga atcgcggtca
      721 ggaacaggat gacgatcccg gccagcaggc tgcgctggcc atcgtcgaga cgtggcggcg
      781 gtgccttctt gccggacgtc gatttcttgg cggcgggctt tttggcggcg ggtttgctgt
      841 gcgaattgct cttcgccttg ctcgtggcgc tcgcctggcg cttggcgctc gtctgacgag
      901 tggtgctcgt cttgtcgttt ttgttactgg tagccatagc tcaaatgttc ccgcggggac
      961 attatagcac gaactacgaa gtatatacta cgcagcgcgt cgaaggggtc gaagggcgag
     1021 acaaacagcc tggctgtacg ggcgggacaa acggcgtgca gaaccctctc caataaccgt
     1081 gacgttggtc gccgtttgtc tcgcccttcc gggtcatcac cgcaattgac tgacaatagc
     1141 aactaccgtc atgcaaccac cgacacagac gacactcgtc attcgtcact cgttcatagt
     1201 ttaacaaagc ggtgaatttt ttgccttccc ccttcccccg cgtgacaatc aaggatcatg
     1261 aaacagtcgc gcctcatcgt cctgatcgct cttgcctttc tggctgcctg tcgccccgcg
     1321 acgggcggag ttacgtccac tgttgtgcct gcgactgctg tgcccacggc caacgtacct
     1381 acgccagcgc ccgccacggc cacggatgaa ccatccgcgc cgccggcaac agatggagca
     1441 tccacgacag ccccactctt cgacatcgac ccgttcgacc gcagtctctt tgccgctggg
     1501 ctgatcagcg acgaagccgg cggcgacgcc ctgcccggcg cgccggtcta tcacatggcc
     1561 atcaccatcg acccgt
//
LOCUS       DAOMQG010000575         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29401_length_1569_cov_5.093791, whole genome shotgun sequence.
ACCESSION   DAOMQG010000575 DAOMQG010000000
VERSION     DAOMQG010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29401_length_1569_cov_5.093791"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..258
                     /locus_tag="PLC06_17315"
     CDS             109..258
                     /locus_tag="PLC06_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92264.1"
                     /translation="MMSLPNIILALTVLLCGAAGLLALLGLLYGLLVAAADTREVPDG
                     NEEYL"
     gene            258..761
                     /locus_tag="PLC06_17320"
     CDS             258..761
                     /locus_tag="PLC06_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92265.1"
                     /translation="MSETTFYLIGIAALCALIFFSAAKTLGEFAFLLSIALIGGGYVA
                     VLVDAFFGISALWAAVVLTALGMIVGRLSSERDIPTVEWRARGFHSTVTACVFSALAF
                     VTFLATPLTPGGTYRLVAVAYGLLAAWNWVVALQMAWWVMTNHDGKSTWNYGDGYRPD
                     DDYGQYD"
     gene            1268..>1569
                     /locus_tag="PLC06_17325"
     CDS             1268..>1569
                     /locus_tag="PLC06_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92266.1"
                     /translation="MSIFNRKQSRYPVSDAQAKERMSRNLKMLAYAVIGGLAVITGFH
                     AVMIVLSETASFTFQGQTGLLLLVMTIFRLGFPLLVEASAVIHTVGAVNGAWKGDQ"
BASE COUNT          304 a          480 c          484 g          301 t
ORIGIN      
        1 gccgccgggg gagccgtatc cggccccgta tccgccgccg ggcgacggct acccggcccc
       61 ctaccccggc ccggtctatc tgcccttcac ggcaggggag agctatccgt gatgagcctg
      121 ccaaacatca tcctggccct aaccgtcctg ttatgcggcg cggccggatt gctcgccctg
      181 ttggggctgc tctatgggct gttggtcgcc gccgccgaca cccgcgaagt gcccgacggc
      241 aatgaggagt atttgtaatg agcgagacga cgttctacct gattggcatc gcggccttat
      301 gcgccctgat atttttcagc gccgccaaga cgctgggaga gtttgccttc ctgctatcga
      361 tcgccctcat cggcggcggt tatgtggccg ttctcgttga cgccttcttc ggcatcagtg
      421 cgctctgggc ggcggtggtg ctgaccgcgc tcggcatgat cgtgggccgt ctgtcgtcgg
      481 agcgtgacat cccgaccgtc gaatggcggg cgcgggggtt tcatagcacg gtcacagcgt
      541 gcgtatttag tgcgttggcg ttcgtgacgt tcctcgccac gccactgacg cccggcggca
      601 cataccggct cgtggcagtc gcttacgggc tgttggccgc gtggaactgg gtggtggcac
      661 tgcaaatggc gtggtgggtg atgacgaatc acgacgggaa atccacctgg aactatggcg
      721 acggctaccg tcccgatgat gattacgggc agtatgacta accaacctac cccccggcga
      781 tagcgccccg ccgggggtga tggcgcagag gagaaaatag cttatgccga tgaacctagc
      841 ggactatcat ccagattgga aaaacatcag ccgacaaata cgtgaacagg ctgggtgggc
      901 ttgcgaggga acgccggact ttcccgactg tcgtgccgtc aatcggcaac gacatccaga
      961 aaacggcagc atcgtcatcc tgacggttgc ccacatgaac cacgagccgg gcgacaacga
     1021 tccggcgaat ctgcgcgccc tatgccagcg ctgccatttg gcatgggaca gagaacatca
     1081 catagcgaac gcccgccggt cgcatgcgag gaagagacgc ggggcgacaa tgcccctgcc
     1141 gttggaggcc ccggccgtgt aggttggccc cctgctctga gaaagtaacg gcgctgcgtc
     1201 aacagcgccg ggcggcgaga acagaccgcc gaactatttt aattcggttt gtaataagag
     1261 gacagaaatg agcatattca accgtaagca atccaggtac cctgtcagcg acgcccaggc
     1321 caaagagcgc atgagccgca acttaaaaat gctggcctat gccgtcatcg gcggcctggc
     1381 cgtcatcacc ggcttccatg ccgtcatgat cgttctatcc gaaacggcca gcttcacctt
     1441 tcaggggcaa accggcctgc tgctgctggt catgacaatc tttcggctcg gattccccct
     1501 gctcgttgaa gcgtcggccg ttatccacac cgtcggcgcg gtaaacggcg cgtggaaagg
     1561 cgatcagaa
//
LOCUS       DAOMQG010000576         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29495_length_1565_cov_8.726490, whole genome shotgun sequence.
ACCESSION   DAOMQG010000576 DAOMQG010000000
VERSION     DAOMQG010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29495_length_1565_cov_8.726490"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1276
                     /locus_tag="PLC06_17330"
     CDS             227..1276
                     /locus_tag="PLC06_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRO92267.1"
                     /translation="MDRTTYGLDIAKNVMQLHWVEAETGEIGRKKLARVRLAAYFAQL
                     QPVRIVMEACGSAHHWARVLGHLGHQVELLPAAQVRAFVRSNKDDAADARAIWLAAQQ
                     SDIRRVPQKSVEQQAVLALHRTRQHWVSVRTATVNSLRGLLYEFGVVLKGGRRAGLKA
                     LAEQRAAIDGQLPATMCTLVDGQLQTLEQIGQRIEQLEREIAALQKQLKGARELDRVP
                     GIGVLGATALAATLGDGKAWRSGREFSASLGLVPAHAGTGGKVRVGHLSKRGDPYLRT
                     LLIHGARAVIEHAKDKPKWLEQLLARRPLNVAVVALANKMARTAWALVAHARAYQRDW
                     QSARPGSGTAQAAAV"
BASE COUNT          315 a          461 c          575 g          214 t
ORIGIN      
        1 cgacggatgc agaacgtcgc ccggatcgat gcggcgccgg atcttccgat gaaaggagat
       61 ggcatggaca cctcccaccc gatgaaccga cgccgatgca gtcgagaggc cgctgaatac
      121 cgctgaggaa ggaaaaggta ggccgtggcg ggggtggggc ctcgcgtcgc acggcatcga
      181 gtgccaaagt gaagctccga gtgaacttcg cagacggagg ccccgaatgg atcgtacgac
      241 ttacggtttg gatattgcca agaacgtgat gcaactgcac tgggtcgaag ccgagaccgg
      301 cgagattgga cgcaagaagc tggcgcgggt gcggcttgcg gcgtacttcg cgcagctgca
      361 gccggtgcgc atcgtgatgg aggcgtgcgg cagcgcacac cactgggcgc gcgtgctcgg
      421 ccacctgggc caccaagtcg agttgctgcc ggcggcgcag gtgcgagcgt tcgtgcgcag
      481 caacaaagac gacgctgccg acgcgcgggc gatctggctg gcggcacagc aaagcgacat
      541 ccggcgcgtg ccgcaaaaga gcgtcgagca gcaggcggtg ctggcgctgc accgcacgag
      601 gcagcactgg gtgagcgtgc gcacggcaac ggtgaacagc ttgagggggt tgctgtacga
      661 gttcggagtc gtgctcaaag gcgggcgccg ggcggggttg aaggcgctgg ccgagcagcg
      721 agctgcgatc gacgggcagt tgcccgcgac gatgtgcacc ttggtggatg ggcagttgca
      781 aacgctcgaa cagatcggcc agcgcatcga gcagctcgag cgcgagatcg cggcgctgca
      841 aaagcagttg aaaggcgcgc gcgagcttga ccgggtcccc ggcatcggcg tgctgggtgc
      901 aaccgccttg gccgccacgc tgggcgacgg caaggcttgg cgctcgggac gggagttctc
      961 cgccagcctc gggctggtgc cggcgcacgc gggcaccggt ggcaaggtgc gcgtgggcca
     1021 cctgagcaag cgaggcgatc cgtacctgcg cacgctgctc attcacgggg cgcgcgcggt
     1081 gatcgagcat gcgaaggaca agcccaagtg gctcgaacaa ctgctggcgc gccggccgct
     1141 gaacgtggcg gtggtggcgc tggccaacaa gatggcacgc acggcctggg cgctcgtcgc
     1201 ccacgcccgg gcgtatcagc gggactggca aagcgccaga cccgggagcg gcactgcgca
     1261 agcggcagcc gtataaatca accaactgaa caaggagtgg acgccgcaaa gagtgcgtga
     1321 gtcgagtcga tgaagtgatg gcaaagcggt cggaccgcgg gccggacaaa gctggatcct
     1381 ggccaggggc atcgctgatg caaaccccgc caatggagat gagcaccggc ctcgcgaatg
     1441 accatcaagg ccagcagcgg ggttgccccg tctgcacctc aaggccggat gtaagacagc
     1501 agtcgtatcc gccgagtcag aacttcaaaa actcgcttgc cacacgggag gtgtccatgt
     1561 aagcc
//
LOCUS       DAOMQG010000577         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29542_length_1563_cov_7.858090, whole genome shotgun sequence.
ACCESSION   DAOMQG010000577 DAOMQG010000000
VERSION     DAOMQG010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29542_length_1563_cov_7.858090"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..884)
                     /locus_tag="PLC06_17335"
     CDS             complement(168..884)
                     /locus_tag="PLC06_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRO92268.1"
                     /translation="MAASRDNNAILALRGVPFFAALPDEDVEHLAKKLVPRRYAAGMA
                     IFHLGDPGGLLYLIGKGKVKISHTTTEGHEVSLAILGPGDFFGEMALIDDAPRSAAAI
                     ALEATETWTLHREEFITYLTDNPEFALHVLTTLARHIRRLNAQLADIFFLDLPGRLAR
                     TLLHLADQYGTYTTEGVIINLSLTQTDLAEMTGATRVSINKALGRFRRAGWIRVTGKQ
                     VTVVDRAGLEGLLVISASEV"
     gene            1195..1560
                     /locus_tag="PLC06_17340"
     CDS             1195..1560
                     /locus_tag="PLC06_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92269.1"
                     /translation="MSTLWRTVLHWTPRVLGALYAVFISLFALDVWGTGAGFMSELIA
                     FLIHLTPVYLIVAALVVAWLYPWVGGILFIALATGFGLFFGRQETSTLLLLALPPTIT
                     GLLFMCDGCLNRIGLQPGV"
BASE COUNT          263 a          443 c          512 g          345 t
ORIGIN      
        1 gattggcatg caatcccgta tttatacacg atctgccgct tttcggcgta atgaattgaa
       61 cgatttatca ttcccagacc tgacaggtcg gcggatcaaa cgactgcgtg gggacgggag
      121 tctgcgccgc ccacctgtca ggtctttata ggtctcgatg ccggcctcta tacctcgctc
      181 gccgaaatga cgagcagccc ttccagcccg gcgcggtcga ccaccgtcac ctgcttgccc
      241 gtgacccgta tccagccggc gcggcggaag cggccgaggg ccttgttgat gctgacgcgg
      301 gtggcgccgg tcatctcggc cagatcggtc tgggtcaggg acagattgat gatcacgccc
      361 tcggtggtgt aggtgccgta ctggtcggcc agatgcagca gcgtgcgcgc cagccggccg
      421 ggcaggtcaa ggaagaagat gtcggccagc tgggcgttga gccgccggat gtgccgggcc
      481 agcgtggtga ggacgtggag cgcgaattcg gggttgtcgg tgagataggt gatgaactcc
      541 tcgcggtgga gcgtccatgt ctcggtcgct tccagggcga tggccgccgc cgaacgcggg
      601 gcgtcgtcga tgagggccat ctcgccgaag aagtcgccag ggccgaggat agccaacgag
      661 acctcatgcc cttcggtggt ggtgtggctg attttgacct tgcccttgcc gatcagatag
      721 agcagcccgc cggggtcgcc aaggtggaag atggccatgc cggcggcata gcggcgcggg
      781 acgagcttct tcgccagatg ctcgacgtct tcgtcgggca gggcagcaaa aaacgggacg
      841 ccccgcaggg ccaatatggc gttgttgtcg cgtgaagcgg ccatcgctcc tcctctctat
      901 ccgcattccc tggctaaatt ttagcgggtt ttatgggaat gacgaatgag ggatgacgaa
      961 tgaagagcgg gctcctgccc ctttctcccc tgctccccca ctggccacga atgacgcgcc
     1021 tttatcgtac ttcatcttca ctcgtcgctc gtgcgatgat tgccgtcgat tgccaaggaa
     1081 atgcgaagcg gctctggttt ttgcaacgaa tgacatttgt cacatgacgg ccgtccgcgc
     1141 cggatgtatg ctataggtag gggtaatgga cttgcccgaa ggaggttaat caaaatgtct
     1201 actttatggc ggaccgtgtt gcattggacg ccgcgcgtgc tgggcgccct ctatgccgtg
     1261 ttcatcagcc tgttcgccct ggacgtgtgg gggacgggcg cggggttcat gtcggaattg
     1321 atcgccttcc tcatccacct gaccccggtt tatctcatcg tggccgcgct ggtcgtcgcc
     1381 tggctttacc cctgggtcgg cggcatcctg ttcatcgcgc tggcaacggg gtttggcctt
     1441 ttcttcggcc ggcaagagac gagtaccttg ttgctgctgg ccttgccgcc gacaatcacg
     1501 ggattgctgt tcatgtgtga cgggtgtctg aacaggatcg gattgcagcc gggagtgtga
     1561 tgg
//
LOCUS       DAOMQG010000578         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29785_length_1552_cov_9.313293, whole genome shotgun sequence.
ACCESSION   DAOMQG010000578 DAOMQG010000000
VERSION     DAOMQG010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29785_length_1552_cov_9.313293"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1470)
                     /locus_tag="PLC06_17345"
     CDS             complement(100..1470)
                     /locus_tag="PLC06_17345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRO92270.1"
                     /translation="MFERLPPVRLKKGQGIAGWVAEHQEPVVINNAYSDQRFFSGVDK
                     RSGFKTSSMICIPLKVNERTIGVLQAINRQFGSFTNHDLELMQGLGGPLAAAIENANL
                     HADVLAEKQRIETLFESIAEGLATVNREGIITKVNTAFSILMTLPADELIGKPLKEVV
                     LTNKGDIDDLIARILNAPEGNVHLAADLNHSRARSLPILISGAPVENQHGSINEAILV
                     FSDLTQTREVERMRDDLFQGIIHELRTPLATILMYSRLLREGKARQPEKTERFLGVIE
                     RESDRLQRMIRQMLDLARLEARELQRSPEPIGLNAVFDELLPSSAESAVRKGLLFRQR
                     IESDLPPVMGHASVYHMTLKHLLDNAIKFTPSGRVQIAAWRENDMVIVEVADDGIGIP
                     PEAMNNLFKRFYRAQSAVERGISGTGLGLHMVKENLRNYNGQIEVQSTLGKGTTFTLR
                     LPIAQI"
BASE COUNT          332 a          418 c          421 g          381 t
ORIGIN      
        1 tccaatatgg agccatggct tcgactactg ccggatggac aggcgtcgtt gcactgtggt
       61 ccagataaat agtgtttcgt tgcatgctgt tacgatgagc tagatttggg cgatcggcag
      121 ccgtagcgtg aaagtggtgc ctttgcccag ggtgctttga acttctattt gcccattata
      181 gttccgaagg ttctctttta ccatgtggag tcccaggcct gtcccggaga tgccccgctc
      241 gacggctgat tgggcccgat aaaagcgctt gaagagattg ttcatcgctt ccggcggaat
      301 gccgataccg tcatcagcta cctcaacgat gaccatatcg ttttcccgcc acgccgcgat
      361 ttgtaccctg ccggaagggg tgaacttaat tgcattatcc aacagatgct tcaaggtcat
      421 gtgatagact gacgcgtggc ccattaccgg cggcagatcg gattcaattc gttgtcggaa
      481 gagcaagccc ttccgaaccg cgctttcggc cgatgacggc agtaactcgt cgaaaacagc
      541 atttaacccg atcggctccg ggctgcgttg caactcgcgc gcctctaacc tggccaggtc
      601 cagcatttgc cgaatcatgc gttgtagccg atcgctctcc cgctcgatga cgcccagaaa
      661 gcgctctgtt ttctcgggct ggcgtgcctt gccctcgcgc agcaaacggg aatacatgag
      721 tatggtggcc aaaggcgtgc gtagttcatg aatgattccc tggaacaggt catcccgcat
      781 ccgctcaact tctcgggtct gggtaaggtc gctgaacacg aggattgcct cattaataga
      841 gccatgctga ttttcgacag gcgcgccact gatcaatatc ggtaatgaac gagcgcgaga
      901 atgattcaga tcggcggcaa gatggacgtt gccttcaggg gcattgagta tccgtgcgat
      961 gagatcgtcg atatcacctt tgttggtgag tacaacctct ttgagcggtt tacctatcaa
     1021 ttcatcagcc ggtaatgtca tcaggattga gaacgctgta ttgaccttgg tgatgatgcc
     1081 ttcccgattg acggtggcca acccttcggc tatgctctcg aagagggtct ctattcgctg
     1141 cttctcggcc agcacatcgg catgcagatt ggcgttttcg atagctgccg ctaatggtcc
     1201 acccagccct tgcattaatt ccaggtcatg attggtgaag ctgccgaact ggcggttgat
     1261 cgcctggagc acgccgatgg tgcgctcatt cacctttaga gggatacaga tcatcgagga
     1321 agtcttgaat cccgaacgtt tgtccacacc ggagaagaaa cgctgatcgg aataggcgtt
     1381 gttgatgacg accggttcct gatgttcggc cacccaaccg gcgatcccct gtcccttttt
     1441 tagccgcacc ggcggaagtc gctcaaacat cgggccaaac aactggccga caaaaacgat
     1501 gtccccggtg attggctcga ccagtccgat cgataaggtt tcaacgttga gc
//
LOCUS       DAOMQG010000579         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_29814_length_1551_cov_6.679144, whole genome shotgun sequence.
ACCESSION   DAOMQG010000579 DAOMQG010000000
VERSION     DAOMQG010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29814_length_1551_cov_6.679144"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLC06_17350"
     CDS             <1..189
                     /locus_tag="PLC06_17350"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRO92271.1"
                     /translation="VKVTMTLTTPGCPLHASFAEEIEQVLWQSIPEITGVTVDLVWDP
                     RWTPMMISAEGMEELGLA"
     gene            204..1211
                     /locus_tag="PLC06_17355"
     CDS             204..1211
                     /locus_tag="PLC06_17355"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRO92272.1"
                     /translation="MQSVQPVPQEHHVISADTAVPATKNQLLDKYALPKVALTVIAIS
                     SLVGTWLTMTTHGAGAWSSVLPRWLHLVSFGLLAGGYMWKGLFTQPAHRPAQSAYVAA
                     FVRAQFARFRRLAWVVLGVYFVTGLLDLVRFSEMGVGWPIWLGAALMVAITLVVMHEL
                     YLRRPDDPFAERPEARLVLGLLVIDALFQATFDVALSQGGAWWPLLVRWLHLTAFGLW
                     IGGAVWNIFITVPAARQTIAIPVVVAAGRQLERFRVVVRMILPALIVTGLIQAYRYVG
                     FSLDALLYSSIGHVILIKIGLVLVLIGVFLTCPLWRACSPISGMCKLDELYDADPSTG
                     R"
     gene            1239..1520
                     /locus_tag="PLC06_17360"
     CDS             1239..1520
                     /locus_tag="PLC06_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRO92273.1"
                     /translation="MTTLASHTLESLVADTTLDNRDGDCASGFVKLLEAMDQLQPGQL
                     MAILSRDPASQRELREWTARAGHDLLEARTSGPFWRREFHYLIRKGGQS"
BASE COUNT          220 a          530 c          491 g          310 t
ORIGIN      
        1 gtcaaggtca ccatgaccct gaccaccccc ggctgtccgc tgcacgccag cttcgccgag
       61 gagatcgagc aggtgctctg gcaatcgatc cccgaaatca ccggcgtcac ggttgatctc
      121 gtctgggatc cgcgctggac gccgatgatg atctcggccg agggcatgga agagctgggc
      181 ctggcctgat tgaggtatcc gacatgcaat ccgttcaacc tgttccccag gaacatcatg
      241 taatttcggc cgacacggcc gttcccgcca ccaaaaacca gctgctggac aagtacgcgc
      301 tgcccaaggt ggcgctgacc gtcatcgcta tctcgtccct cgtgggcacg tggctgacca
      361 tgaccaccca cggcgcgggc gcgtggtcgt cggtcttgcc gcgctggctc catctggtgt
      421 ccttcggcct gctggccggg gggtatatgt ggaaggggct gttcacccag cccgcccatc
      481 gcccggccca atccgcctac gtcgccgcgt tcgtcagggc gcaattcgcc cgcttccggc
      541 ggctggcctg ggtggtgctg ggcgtctact tcgtcaccgg cctgctcgac ctcgttcgct
      601 tcagcgagat gggcgtcggc tggccgatct ggctcggcgc ggcgctgatg gtggccatca
      661 ccctggtggt gatgcacgag ctgtacttgc ggcggcccga cgacccgttc gccgagcggc
      721 cggaggcgcg cctggtgctg ggcctgctgg tgatcgacgc gctgttccag gcgacattcg
      781 acgtggccct gtcgcagggc ggcgcgtggt ggccgctgct ggtgcgctgg ctccatctga
      841 ccgccttcgg tctgtggatc ggcggcgcgg tgtggaacat cttcatcacc gtgcccgcgg
      901 cgcggcagac catcgccatc ccggtcgtcg tggctgccgg ccggcagctg gagcggttcc
      961 gcgtggtcgt gcgcatgatc ctgccggcgc tcatcgtcac cggcctcatc caggcctatc
     1021 gctacgtcgg cttcagcctc gacgcgctgc tctactcgtc catcggccac gtcatcctga
     1081 tcaagatcgg gctggtgctg gtgctgatcg gcgtgttcct gacctgcccg ttgtggcggg
     1141 cctgctcgcc catttcgggc atgtgcaagc tcgacgagct gtatgacgcc gacccctcga
     1201 ccggccgcta atcgattatt tttcaggagt ccaatcctat gacaactctc gcatcccaca
     1261 cccttgagtc cctcgtggcc gacacgacgc tcgacaatcg cgacggcgat tgcgcttcgg
     1321 gcttcgtcaa gctgctggaa gcgatggacc agctccagcc tggccaactg atggccatcc
     1381 tcagccgcga ccccgcttcg cagcgggagc tgcgggaatg gaccgcccgc gccggccatg
     1441 atctgctgga ggcgcgcacg tccggcccgt tctggcggcg cgaattccat tatctcattc
     1501 gcaagggagg tcaatcatga ccgtactgaa tcctgccgac gccatcgtgg t
//
LOCUS       DAOMQG010000580         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_30151_length_1537_cov_5.890013, whole genome shotgun sequence.
ACCESSION   DAOMQG010000580 DAOMQG010000000
VERSION     DAOMQG010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30151_length_1537_cov_5.890013"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..>1537)
                     /locus_tag="PLC06_17365"
     CDS             complement(225..>1537)
                     /locus_tag="PLC06_17365"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRO92274.1"
                     /translation="PLTVKTHLRNIFIKLDVSTRTEAMTVAIQQGVLNVGGGDGSPVV
                     VGQSPDAVAEEADDWPAPTEAGTNGQAPAEEAEPTPAAPVVSHRWRNLSLALLALLLL
                     TAALFAYIQWRGDGLPPAASEPFVEQELGETRWLSSRPLPEPRAGRAAAAVGLEVYVI
                     GGETEAGITDDTRVFNTLDRTWRRAADKPTAVGDVSAAELFGEIYVPGGLLADGKPTS
                     LVEAYSPTQNAWRRAAALPRPVAGAVVLADGGFLYVFGGRDENGPLDTAFVYDPAADS
                     WRPIAPLPEPRAEAAGGALLGRLYVVGGVDDAGISDACSVYDPAADAWSECPTMLQPR
                     AGAGATVLLNKLYLIGGMAAAASDEVSYGEVYDPNSRAWTILNMPPDAVTWTEPGVTH
                     VETRIYALGGRKGDALSDSTLVYAPLVFQTFIPAAPSGGDNGDE"
BASE COUNT          243 a          540 c          518 g          236 t
ORIGIN      
        1 gcgccaggaa gatcagcagc gccagattgc ggcgcaaaaa ccgcaacacc ggccgatcga
       61 ttgggtcttc atcatcgtaa gccgcgcgca ttatgtcgat attgtaaccg attcgacctc
      121 gaaatacgag acccggccat ggacatagat ccggcggctg tggcgcaagt tggcagcttg
      181 cggtcgggac gaaggggcaa gttgccaact tgccctacag gcatctactc gtccccgttg
      241 tcgccgcccg aaggcgcggc cgggatgaaa gtttggaaga cgagcggcgc atagaccaac
      301 gtactgtcgc tcagcgcgtc tcccttgcgg ccgcccaggg cgtagatgcg cgtctcgacg
      361 tgggtcacgc ccggctccgt ccacgtcacc gcatccggcg gcatgttgag gatcgtccag
      421 gcgcggctgt tggggtcata gacctcgcca tagctcacct cgtcggaagc ggcggcggcc
      481 atgcccccga tgagatagag cttgttcagc agcacggtcg cccccgcacc ggcgcgcggc
      541 tgcagcatgg tcgggcactc gctccaggcg tcggccgccg gatcatagac ggagcaggcg
      601 tcgctgatgc ccgcgtcgtc caccccgccc accacgtaga gccggcccag cagcgccccg
      661 ccggcggctt cggcgcgcgg ctccggcagc ggcgcaatcg gccgccagct atcggcggcg
      721 gggtcgtaga caaaggcggt gtccagcggg ccgttctcgt cgcggccgcc gaacacatag
      781 aggaagccgc catcggccag gacgaccgcc ccggccaccg gtcgcggcaa tgccgccgct
      841 cgtcgccagg cgttctgcgt cgggctatag gcctcaacga ggctcgtcgg cttgccgtcg
      901 gccagcaagc cgcccggcac atagatttcc ccgaacagct cggcggccga tacatccccc
      961 acggcggtgg gcttgtcggc cgcgcgtcgc caggtgcggt ccagcgtgtt gaacacccgc
     1021 gtgtcgtcgg tgatgcccgc ttcagtctcg ccaccgataa cgtagacctc cagccccacg
     1081 gcggccgcgg cccggcccgc gcgcggttcc ggcagcgggc ggctgctcag ccagcgcgtc
     1141 tcgcccagct cctgctcgac gaacggttcg ctcgccgccg gcggcaaccc atcgccgcgc
     1201 cactgaatgt aggcgaacag ggccgcagtc agcagcagca gagccaacag cgccaggctc
     1261 agattgcgcc agcgatggga gacgaccggg gcagcggggg tcggttcggc ttcttcggcg
     1321 ggggcctggc cattggttcc ggcctcggtc ggggcgggcc aatcgtccgc ttcttcggcg
     1381 accgcgtcgg gcgattggcc gacgacgacc ggtgagccgt cgcccccgcc cacgttgagc
     1441 accccctgct ggatggcgac cgtcatcgcc tcggtgcgcg tggacacgtc cagcttgatg
     1501 aagatgttgc gtaaatgggt cttcaccgtg agcgggc
//
LOCUS       DAOMQG010000581         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_30673_length_1516_cov_9.932923, whole genome shotgun sequence.
ACCESSION   DAOMQG010000581 DAOMQG010000000
VERSION     DAOMQG010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30673_length_1516_cov_9.932923"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="budA"
                     /locus_tag="PLC06_17370"
     CDS             <1..582
                     /gene="budA"
                     /locus_tag="PLC06_17370"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HRO92275.1"
                     /translation="LLDGEMVMLDGRIYQMTADGCVQEVGDDATTPFACVTFYRPEKT
                     FDLDGELSHDDFLRRLQESLPSPNIFYAFRITGEFAHMKVRSVPKSECYIPLVEIAKE
                     QPVFEFENISGTLAGFYTPEFMASVSVPGMHLHFLSDDLAHGGHLLSCRPRHVSAGVQ
                     ALHTVELALPTTSYYLEWDFRRDVARDLEKAEK"
     gene            complement(846..>1516)
                     /locus_tag="PLC06_17375"
     CDS             complement(846..>1516)
                     /locus_tag="PLC06_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRO92276.1"
                     /translation="MTTLIPRNTTIPSRKSETFSTAEDNQTAVDIHILQGERPVAKDN
                     NTLGVFRLEGLPPAPRGVPQIEVTFDIDANGILNVTAKDRATGRSQQVTITASTNLNE
                     SEIQRMVKEAESHAGEDQQRRALIEARNAADQVIYQTEKSLGQMNGNAPADTRTRIDG
                     LIAELREATQTEDTDRIRRLTEALQQTVMSLGESMAQQQAGPEAANANGHDEDVVEGE
                     FESA"
BASE COUNT          269 a          479 c          453 g          315 t
ORIGIN      
        1 ctgctcgacg gcgagatggt catgctcgac ggccgcatct accagatgac ggccgacggc
       61 tgtgtgcagg aagtgggcga cgacgcgacg acgcctttcg cctgtgtcac cttctaccgg
      121 ccggagaaga cgttcgatct cgatggcgaa ttgagccacg acgacttcct gcgccgcctg
      181 caggagtcat tgccctcgcc caatatcttc tacgccttcc gcatcaccgg cgagttcgcc
      241 cacatgaagg tgcgctccgt gcccaaatcg gagtgctata tcccgctggt ggagattgcc
      301 aaagagcaac cggtcttcga gttcgagaat atcagcggca cattggccgg cttctacacg
      361 cctgagttta tggcctccgt cagcgtgccg gggatgcact tgcatttcct gtcggacgac
      421 ctggcccacg gcggccactt gctcagttgc cggccgcgcc acgtgagcgc cggcgttcag
      481 gccctgcaca cggtcgaact ggcgctgccc acgacatcgt attatctgga atgggacttc
      541 cggcgggacg ttgcccggga tcttgaaaaa gcggagaagt aacgggcaac gaaaaaataa
      601 atccgcgggc cgggaagagg aatctgctcg cggcccgcgg gcaacagact gcacgatatt
      661 cgggtgacag tctgaatggg aaagcatacg aattttaacg gagcctgcgc gcgctgtcga
      721 acgcaaggcc tccataaaca gacaacggcc ggctggataa actccaaccg gccgttgccc
      781 ttagcaagga gaacaatcct ccgtcaccgg tgtctctcga tgaccggtga cggtcatagc
      841 tcacgttacg ccgattcgaa ctcgccctcg acgacatctt cgtcatggcc gttggcatta
      901 gccgcttcag gccctgcctg ctgttgagcc atgctttcgc ccagcgacat gaccgtctgc
      961 tgcaacgcct cggtcaggcg gcggatacga tcggtatctt cagtctgagt cgcctcgcgc
     1021 aattcggcga tcaaaccatc aatcctcgtg cgcgtatctg ccggggcatt gccattcatc
     1081 tggcccaggc ttttctcagt ctgataaatc acctggtcgg ccgcgttacg cgcctcgatc
     1141 aacgcccggc gctgctggtc ttcacccgca tgcgactcgg cttccttcac catgcgctgg
     1201 atctcgctct cgttcaggtt cgtgctggcg gtgatggtca cctgctggct gcgacccgtg
     1261 gcgcggtcct tggcggtcac gttcaggata ccgttggcgt cgatgtcgaa cgtcacttcg
     1321 atctgcggga cgccgcgcgg cgcgggcggc aggccctcga gccggaagac gcccagcgtg
     1381 ttgttgtcct tggccaccgg ccgctcgccc tgcaagatgt ggatgtccac ggccgtctga
     1441 ttgtcctcgg ccgtgctgaa cgtctcgctc ttgcgcgacg ggatggtcgt gttgcgcggg
     1501 atcagcgtgg tcatca
//
LOCUS       DAOMQG010000582         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_30730_length_1514_cov_10.715559, whole genome shotgun
            sequence.
ACCESSION   DAOMQG010000582 DAOMQG010000000
VERSION     DAOMQG010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30730_length_1514_cov_10.715559"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1136
                     /locus_tag="PLC06_17380"
     CDS             30..1136
                     /locus_tag="PLC06_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92277.1"
                     /translation="MSLNKASNSIFVSSCRADWGLINLVTMVRPFDLRDLALLRRTGE
                     RGVSLHAVSALVENFHPLRSAIVSMLIGGEFPTFVWKPDNGENAGFIQLRIPASATQA
                     YVLYISPRCQPPADGAAAMSIPAIDEAALDEPGCSAWLALLDEAAAGLGSRGVHHIIA
                     EVDEHGVELQALRRAGFAIYTRQDIWTMRAADYKPQALPNHPLTRRVSTDEWDVQLLY
                     ANTVPRLVQAVEPLPINGEGEGWVLREGDELAAFVNVRRGAVATWLRFFIHPEAESEA
                     ADIVAAALQVAFANESELVYCCVRRYESWSSNTLEHGGFHLVGSQAVMVRHIVQHLPR
                     SVPEAAVALEGQRISASSPIVRNFRKANHKNGSL"
     gene            complement(1201..>1514)
                     /locus_tag="PLC06_17385"
     CDS             complement(1201..>1514)
                     /locus_tag="PLC06_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRO92278.1"
                     /translation="IDRPINCSPHAASQADNLAMAIANAGDAVQCARNTGPIIIVKVT
                     NAIDNLRNLLVRNFGIPQDALTIDVSGGGQTAEIHDDLKQLVLTAGLLHRHANTAGQG
                     R"
BASE COUNT          287 a          394 c          463 g          370 t
ORIGIN      
        1 gcgtcataac tctatgaatt tttattaaaa tgtccttaaa taaggcctct aatagcatat
       61 tcgtctcttc ttgtcgggca gactgggggt tgataaactt ggtgacaatg gttcggccct
      121 ttgatctgcg cgatctggct ttgcttcgcc gaacgggcga acgtggtgtc tcgctgcacg
      181 cggtttcggc gctggttgag aactttcatc cccttcgcag cgcaattgtc agtatgctga
      241 taggcggcga gttccccact ttcgtctgga aaccggataa tggcgaaaat gccgggttca
      301 tccagttgcg aatacccgcg agtgcgactc aggcgtacgt cctgtatatc agtccgcgat
      361 gccaaccgcc cgccgatggc gccgcggcta tgagtatccc agccatcgat gaggcggcgc
      421 ttgacgagcc gggctgcagc gcctggcttg cgctgctcga tgaagcggcg gccggcttgg
      481 ggtctcgcgg cgttcatcac atcattgctg aggtcgatga acatggcgtg gagttacagg
      541 ctctgcggcg tgccgggttc gcgatttata ctcgtcagga catctggacc atgcgggccg
      601 ctgattacaa accacaggcg cttccgaatc acccgttaac gcgtcgtgtc tcgaccgatg
      661 aatgggatgt tcagctcttg tatgccaata cggtcccgcg tctggtccag gctgtagagc
      721 cgttgccgat taatggcgag ggcgaaggat gggtgctgcg cgagggcgat gaactggcag
      781 cgttcgtgaa tgttcgtcgt ggcgcggtcg ctacctggtt gcgttttttc atccacccgg
      841 aggctgaatc ggaggcggcc gatatcgtag ccgccgccct gcaggtcgct tttgccaacg
      901 aatcggagct tgtttactgt tgcgttcgcc gttatgagag ttggtcgtcg aatacattgg
      961 aacacggcgg ctttcatctt gtcggcagtc aggccgtaat ggtccgccat atcgttcagc
     1021 atttgccgcg atcggttcct gaggcggctg tggctttgga gggacagcga atatctgcat
     1081 cttctccgat agttcgaaat ttcaggaagg cgaatcataa gaatggaagc ctctaggtca
     1141 cgaggttctt tacaggcaga aatggattct atacagtcgc acgaagactt acgcacatta
     1201 ctatcggccc tgcccggcag tattcgcatg gcggtggagg aggccggccg tgaggacgag
     1261 ctgcttgaga tcgtcatgga tctcggccgt ttgcccaccg cccgatacgt cgatggtgag
     1321 cgcatcctgc gggataccga agttacgcac gaggagattg cgcaggttgt caatggcatt
     1381 ggtgactttg acgatgataa tcgggccggt attgcgcgca cattgcaccg catctccggc
     1441 attcgcaatc gccatcgcca ggttgtcggc ctgacttgcc gcgtggggcg agcagtttat
     1501 gggacgatcg atat
//
LOCUS       DAOMQG010000583         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Promineifilum sp. isolate AMR_MDS_5840
            NODE_30997_length_1504_cov_5.768806, whole genome shotgun sequence.
ACCESSION   DAOMQG010000583 DAOMQG010000000
VERSION     DAOMQG010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32868061
            Sequence Read Archive: SRR1763384
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Promineifilum sp. (wastewater metagenome)
  ORGANISM  Promineifilum sp.
            Bacteria; Bacillati; Chloroflexota; Ardenticatenia; Candidatus
            Promineifilales; Candidatus Promineifilaceae; Candidatus
            Promineifilum.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.241
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 22:10:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,479
            CDSs (total)                      :: 3,446
            Genes (coding)                    :: 3,418
            CDSs (with protein)               :: 3,418
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 1 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Promineifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30997_length_1504_cov_5.768806"
                     /isolate="AMR_MDS_5840"
                     /isolation_source="Activated sludge and aerobic granule"
                     /db_xref="taxon:2664178"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.2 N 6.14 E"
                     /collection_date="2014-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLC06_17390"
     CDS             complement(<1..633)
                     /locus_tag="PLC06_17390"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRO92279.1"
                     /translation="MWGGGAAAKGQCAVPGRQLAFAQGEADATAVVVGEVVAAGMPAA
                     VVADFSGRLVETTDLIERDVVGVAAGFPAEVRVDALPDVTLSGAVRSIGGIARDVRGD
                     TTYPITIQLDDTAGVPLRWGMTVFVTIDTDGQATPLVAATAEVVPAGAEIAAEGVLVP
                     LETADLAFQTGGIVAELPVAEGATVAAGDALIRLDDAAVTAAVAEAEAGLA"
     gene            923..1333
                     /locus_tag="PLC06_17395"
     CDS             923..1333
                     /locus_tag="PLC06_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRO92280.1"
                     /translation="MKHVSIARRRLFAVALFLCLLPLLAACGGAEAAALPPTPTPDPV
                     LVAGKKVFVAHCGACHSTEPDSIIVGPSLAGVAEAGATRVDGLDARAYVYNSIMRPSD
                     YLVAGFDDLMPQDLARKLTGEELDSVAAYVLSLN"
BASE COUNT          268 a          492 c          475 g          269 t
ORIGIN      
        1 ggccagcccg gcttcggcct cggccacggc ggcggtcacg gccgcgtcgt cgagccggat
       61 gagcgcgtcg cccgcggcca ccgtcgcccc ttcggccacc ggcagctcgg ccacgatgcc
      121 gccggtctgg aaggccagat cggccgtctc cagcggcacg aggacgccct cggcggcgat
      181 ctcggcgccg gcgggaacca cttcggccgt ggcggcgacg agcggcgtcg cttgcccgtc
      241 ggtgtcgatg gtcacgaaga cggtcatccc ccagcgcagg gggacgccgg cggtatcgtc
      301 taactggatg gtgatcgggt aggtggtgtc gccgcgcacg tcccgggcga tgccgccgat
      361 ggagcgcacc gcgccggaga gcgtcacgtc gggcagggcg tcgacgcgca cttcggccgg
      421 gaagcccgcg gccacgccga cgacgtcgcg ctcgatcagg tcggtcgtct ccaccagccg
      481 gcccgaaaaa tcagctacga cggccgcggg catgccggcg gcgaccacct cgcccacgac
      541 aacagcggtc gcatccgctt cgccttgggc gaatgcgagc tgacggccgg ggacggcaca
      601 ttggcccttc gcggccgcgc caccccccca catttgtgac aagttgcaat ttcttgccct
      661 ctccgcgcct cattcgtaca attcctccac tcaacagtct ggaaaccggg tttcgcggga
      721 agagcacggt tccggcatat cacactattg aacaaggttt cttaagttcg tagcaagcgg
      781 ctttagccgc gctccgggtt tgtagttagc ggctttagcc gcgttctccc cggaacggcg
      841 ctaaagcgcc tgactacgaa caataagaga caatgtcata tccgctcaac gggagcagtt
      901 tattgtgggg ttggtgagtc caatgaagca tgtatccata gcgcgccggc ggctgttcgc
      961 cgtggcgctt ttcctgtgtc tgctgccatt attggcggcg tgcggcggcg cggaagcggc
     1021 cgctctcccc ccaacgccca cgccggaccc ggtgctggtc gccgggaaaa aggtgttcgt
     1081 cgcccattgc ggcgcgtgcc acagcacgga gccggattcg atcatcgtcg gcccgtcgct
     1141 ggcgggcgtg gccgaggcgg gggcgacgcg cgtcgatggc ctcgacgccc gcgcctacgt
     1201 ctataactcc atcatgcggc cgtcggacta cctcgtcgcg ggcttcgacg acctgatgcc
     1261 ccaggacctg gcccgcaagc tcaccggcga agaactggac agcgtggcgg cgtatgtgct
     1321 gtcgttgaat tgaattacga attacgaatt acgaattacg aatgaagaga tgatcactga
     1381 tcgactgacc tactgatcta ctgactactg atctactgac ccactgaccc actgatctac
     1441 tgacctaccg atcactaaaa aggaggagca atgcgcaagg tactcaagat attcggaatt
     1501 atta
//
